LOCUS       DAOQQY010000001        38597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_294_length_38597_cov_7.864434, whole genome shotgun sequence.
ACCESSION   DAOQQY010000001 DAOQQY010000000
VERSION     DAOQQY010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 38597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38597
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_294_length_38597_cov_7.864434"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1753)
                     /locus_tag="PLE23_00005"
     CDS             complement(83..1753)
                     /locus_tag="PLE23_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX81673.1"
                     /translation="MLKFDIPLLNKLFCIRITCIIILYVADSTLVVNAQNVAIEKLGE
                     TVNTDIYQEISPVLSPDGSILYFTRSGSPDFEKYLELNDRNLYETNPETRYMELLADI
                     YRQLGDNEVRNPHRSSFNQDVWIATSADGKKFDKVYHPGPPLNNALPNSISSLTPVNN
                     RFVIINQFPKGGGMLKGFSTIDKKNDTEWYYPKPLYIDNYFNNSDIVSFTMSADGKVA
                     VLSLKRDDSLGENDLYICQHIKDSLWSEPVNLKAVNSAANEISPYLTEDGRTLFFASS
                     RPGGYGGLDIYMATKPDTDWEQWTTPKPLHENINSAKDDFQPHYNSSTGYLYFSTRRA
                     GSSDIYRAKIGDQNPVKINIFGKVIDSKTLRPVRSSVYIGNESEDNYRKMFETSTGEF
                     TTVAGKNDKIKIFLFNENYLSQPYIIDVSALQIGYSNSVEINFYIDPKVVDGRISMNP
                     IYFVQSKPEILQKSKPELEKLAELLRNNSSLCIRIEGHTDNLGKVEDLLKLSQERAES
                     VKSFLIQSGIDGARLTTKGYGASQPVNDNASVELREKNRRVEFIITKI"
     gene            1842..3158
                     /locus_tag="PLE23_00010"
     CDS             1842..3158
                     /locus_tag="PLE23_00010"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMX81674.1"
                     /translation="MDFLKKILDTHLFSTDGYKLSLQDLLLFTAIFIAFIFMLRFVMR
                     RLLPRFFKIYDVGNAGKSKIKSATFVVLSLIFILAALWSLHLDAVLYSSDYITIKVSN
                     ILQAILVIRLARILDNIINKIKNKNQDEELRVTTPGGDTVEKNQAHIIQWLVYLIAAL
                     ILVNFFEIDKPLVTIKGFSLKISSILIVIIILFGSRLLAWFLTNILLQPYYARNKIER
                     GAQYAVNQLMSYIIYLVAFFFALHVIDVQMSLILGGAAALLVGVGLGLQQTFNDFFSG
                     ITLLTERSIDIGDVVNINNTVGTVRHIGIRTSKIETRGNLTMIVPNSKLTSDNVINWT
                     HFNNLARLEVKVPVPFSADPELVRTILLKAILPDPRIQKYPEPSVVFNDFGQYAHDFI
                     LMAWSTDVLNFEDIKSELRFEIKRLFKENGIEIPYPIQFYANIPES"
     gene            complement(3155..3865)
                     /locus_tag="PLE23_00015"
     CDS             complement(3155..3865)
                     /locus_tag="PLE23_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMX81675.1"
                     /translation="MYSIEDDKKLGLSVSQEIASKPNEYPLLPEQGNEEVYRFIRGIK
                     DNILNSGRVKNKKNFAWEVKIIKDDKVLNAFCTPGGYIYVYTGLIKYLDTEDQLAGVL
                     GHEIAHADMRHSTRQLTKVMPLAVVGEVLAGNNKNKAAIANITTTLASLSFSRDHEKE
                     ADKYSVYYLCNSGYDGAGAAGFFKKIKGKNSTPQFLSTHPDPGNRVKAIEEQAAKLHC
                     SNYSDKPQTNYQRIKSLL"
     gene            complement(4014..5132)
                     /locus_tag="PLE23_00020"
     CDS             complement(4014..5132)
                     /locus_tag="PLE23_00020"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HMX81676.1"
                     /translation="MRSVVVICLLVWLKCVSFAQKTDNIAITPQLVYGKMLKHSPKIL
                     FRVPDYSVGVAANVVYRTYGSQYWNSWQKYPELGVSFGYFHLGDDHILGSAFSLLPNI
                     SLKLGGADKTQFRFLVGTGVAYLTKKFDYTDNPLQTAIGSHINNITVLQFNWHRRISN
                     EVKMLGGFGLTHFSNGSSKLPNLGINLVTAQLGLQWTPNVVVPSEYEVSDVDPDPTHR
                     FGLSLHGGLAFVEQSLPGGPKYPVYIASLGGIYRLSKANQLLLGTEIEYHSADYYFLL
                     RSTEASSEKIASQAATRHLVFVAEEFFFWPFSIMLQAGTYLDTDFDYRFGKIFTKLTS
                     RYYFPEMYKTRVRFYGGVQLKAHKFAAEYISFGVGAML"
     gene            complement(5129..6625)
                     /gene="hutH"
                     /locus_tag="PLE23_00025"
     CDS             complement(5129..6625)
                     /gene="hutH"
                     /locus_tag="PLE23_00025"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HMX81677.1"
                     /translation="MRDQHLLTLESYTINDIASIIDRECEIKLSEEAIEAVQKCHNFL
                     QSKISSGNQAFYGINTGFGSLCNHVIGKDQLRDLQKNLVLSHACGTGDSVPANVVKII
                     LLLKIRSLAYGHSGVSKEVIDRLIFFYNHDILPVIFQQGSLGASGDLAPLAHLSLPLI
                     GEGNVIYQGKIRPTEEVLAMFGLNPLDLGAKEGLALLNGTQFSTGYGVYAIIEAQEIM
                     RWANCVGAMSAEAFLCSATPWDDKLHKIRNQPGQQTVAGQIRKWLEGSPLLETDRVAV
                     QDPYAFRCIPQVHGATYDTIQYVMSVLENEVNAVTDNPNIFVEEDQILSGGNFHAQPI
                     ALVLDFLAISLSELGSISERRTYQLISGLRNLPVFLVKNAGLNSGFMIPQYTAASIVS
                     QNKQLCTPASVDSIVSSNGQEDHVSMAANAATKLYRIVENIWTILAIEWLNACQAIEF
                     RRPARSSEALEYLHDLFREQVSRMDNDRQLSKDIEATRIFLKEHSHQV"
     gene            6756..7799
                     /locus_tag="PLE23_00030"
     CDS             6756..7799
                     /locus_tag="PLE23_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HMX81678.1"
                     /translation="MAENLQDKNIIASDPTAESRKQDHIDLAFRSQVDVLRADGRFYY
                     EPLLAGFSDKDIYPQTQLLGKTFRYPMWISSMTGGNKMAGKINENLARVCNDFGLGFG
                     LGSCRQLLTDHTYLKDFDVRKYLGDRPFYANLGVAQIEELLINNKVDEIIRLVDLLKA
                     DGLIVHVNPLQEWSQPEGNLYTQRPIETIEKLLEKSKLNIIVKEVGQGMGKKSLEALV
                     KLPLAAIDFAAFGGTNFSKLELYRNPEKQKAHEVMSLVGHTAEEMVSMVNEILSHSTD
                     MIRCREFIVSGGIRSYLDGYYLTRNINAASIYGQASGFLKHAMEDYDQLYRYVKAQTE
                     GLELANTFLTIKE"
     gene            7809..9155
                     /locus_tag="PLE23_00035"
     CDS             7809..9155
                     /locus_tag="PLE23_00035"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HMX81679.1"
                     /translation="MKDDKQINGFSKRSKAEKLQWVIENFFNGHESAAGELRSFWLDD
                     ETQNIFDAFSENSISNFILPYGVAPNMLINGKTYCVPMVTEESSVVAAAAAAAKYWST
                     RGGIKATVVNTKKIGQVHFKWSGQTEKLISVFPELKKELIITASAITTNMDKRGGGIL
                     DIELKDLSDKEPDYYQLFTHFDTCDSMGANFINSVLEQFGRTLRLFIQTHPAFDETDR
                     EVDIIMCILSNYTPECLVKAEVSCTIDELGDECLEMDAVGFAERFATAIRIAEIDSYR
                     ATTHNKGIFNGIDAVVIATGNDFRAIEASGHTYAARSGIYKSLSKCTIKDNIFSFNLE
                     IPLAIGTIGGLTKLHPIARHSLEMLGHPSAPELMTIIAATGLMQNFAAIRSLVTTGIQ
                     KGHMKMHLMNILNHLSATEQEIEQAFEYFSDKVISFTSVRELITEIREREHKIITD"
     gene            9168..10094
                     /locus_tag="PLE23_00040"
     CDS             9168..10094
                     /locus_tag="PLE23_00040"
                     /inference="COORDINATES: protein motif:HMM:NF040656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYDIA family GHMP kinase"
                     /protein_id="HMX81680.1"
                     /translation="MKKNFSANGKLLLTAEYLVLEGALALALPVRFGQKMSVTENSSS
                     EIKWSSLDHKDELWFSASFSLYDFKATETTDEEIAARITKLLKGCARQDSEFLSQWKG
                     QKVETKLEFDRKWGLGTSSTLVYCIAEWADVNPYQLLMDTFGGSGYDIACAGADGPVL
                     YSLRDYAISVEESLFYPSFRNNLFFIYSGRKQNSQAAVKDFQRLKNFSTKDIDTASEL
                     TESFEKCTNLHDFKKLITEHELLISKILKTSPMKNEYFHDFEGEIKSLGAWGGDFILA
                     ATESDKKYVTEYFTGKGLTDFFSYDDLILKRK"
     gene            10097..10822
                     /locus_tag="PLE23_00045"
     CDS             10097..10822
                     /locus_tag="PLE23_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMX81681.1"
                     /translation="MPPKWIEAKLTEIKPVTDKIRNFYLTLNDEDRFGFLPGQFVTFD
                     LPVSEKRLQRWKSYSIANRPDGSNTLEFCIVHLDGGLGSGYLFNEVQPGSLLRFKGPD
                     GIFTLKEPLDYDLVMLCTGTGVAPFKSMLDHIFHHNIPHRQLHLIFGTRKEEDILYRK
                     EFEEYAAVHPEFRYDVVLSRQENWPGYKGHIHQVYLKEYAETRPDVRFMICGWSQMSD
                     QATEYLTQKCGYEKSQVHCELYG"
     gene            10880..11488
                     /locus_tag="PLE23_00050"
     CDS             10880..11488
                     /locus_tag="PLE23_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMX81682.1"
                     /translation="MEPTIKSPVMLYTEQTPNPESLKFVTNRMLYHGTADFKDKESAQ
                     EWSPMALALYDLPYVRGVYINNNFVTVTKEINYNWDDIMLRIKDFIKKYVESEAVVIN
                     DGYAEAMEKQAKIEQETPYEGDEAELVKKIVNIIDTYVKPAVGLDGGNIEFKKYEDGV
                     VYVIMQGSCSGCPSSTLTLKAGIEGMLKRMVPQVREVVQEMG"
     gene            complement(11510..14710)
                     /locus_tag="PLE23_00055"
     CDS             complement(11510..14710)
                     /locus_tag="PLE23_00055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX81683.1"
                     /translation="MNKLLLLTFFVFGSAIFQAQVPDCVQCCCGTPIEVGVPGGNFED
                     PPYANPIIVYFAGQMYSSWTVNSGSIDLLGPNYLNWATGNPNGATQFIDLHGNTPGSF
                     STILNGLNVDYEYTIVLWYAKNAITPTANCQIQVADGAWLDQTWTATNNGANGWLRKC
                     FTFTALASTADLKFTGTGPVVNGGVLLDDITMYSCPPKSIPAFTDMPDDISGIQCLKD
                     VPPPPMLTVQDDCDNNPSIDYSESTTGTPCNQTITRTWTATNSCGATSSVVQQVFIED
                     NEAPAFVDEPQDIYVDCSLNIQAEFNQWLNGNGGATATDNCEGNVLWSYEYQNEPEFP
                     CSETEVSFIVSDNCGNENTKTAKFIVEDTEDPVITKQPENIELICDADPYAKLTQWLN
                     NHANATVSDNCSSVTWSNNFDNNYTKTLYTIQFTATDECGNFTTFSATFNIKPGGQTV
                     NLQKTTCDSSQAGIDTLIYNVNGCDSIVITTTLLVLADTLKIFGTTCIKSKALSDTLI
                     LQNSMGCDSIVIHQIKYNKPDTTIIENQSCSINNFSVDTLSLQGKYCDSLVLIKNIPL
                     LKDTVYFSKNTCDTTKAGIDTTQYLNQYGCDSIVITELKFSGYLSSELIVHQCGDDPD
                     YLDTIIYKTAECDSFVFIKYIYHKPDSVNQQQGTCDPKLTGTTQYNYKNSLGCDSLVF
                     VTRYLLPSDTVKQYKNTCQKSDAGVFELKYTNQFGCDSIVILTSTLILPDTTYVSATT
                     CDPTLAGISIQIIKTAECDSIVITNTSLLPSYFIKKQNYSCTAQNIATDTTKLLSISG
                     CDSIIVVETIPKPLDVFQLVKPEKCKDSNDGSIVIDSVHNGLPPYQYSINGIDYFPEN
                     EFSKLAPGDYTLYIKDNQGCTAEIKNLTIDKGSTFTVELGPDKKVKEGTLIDLIPVYS
                     DTAYQIQWAPASVFSCIACKTNSLIINHDQKIFLYAENQHRCQASDSILLSVIPDIKV
                     YIPNVFSPDLDGINDYFTVYGDHHLVSIKELNIYSRWGEHLFSAKNIEPNNPSLGWNG
                     KTRGKLLDPGVFVYWAVLEFDNGETALYRGELILLR"
     gene            complement(14802..18164)
                     /gene="mfd"
                     /locus_tag="PLE23_00060"
     CDS             complement(14802..18164)
                     /gene="mfd"
                     /locus_tag="PLE23_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HMX81684.1"
                     /translation="MQNLTKLLEKYSQDSRILKLASLLTASAPQAITLEGLAGSSVAF
                     VIAGSWMSNPRQMVIIANNQEEAAYMFNNLDNIFTLTKVHYLPDSFKKPGNFDQISST
                     QVLQRTEVVNYISESDHDQSIIITYPEALFEKVVSPDSLKDHRIIITKSEPFDAGFLI
                     EILSEYGFERTDFVFEPGQFSVRGGILDIYSFGNEWPYRVELYGDDVESIRLFDPVTQ
                     LSQKTVSKVVIVPHVGKKFEHNQKQSFFDLLAKNAILYIADEKNLVDVLQNCFEASEN
                     IGEKIRFMDDDEKTDFIRDRSFIRPGDVISASQQFTRIGYAGRLSAGGDNILFGIKPQ
                     PSFNKNFKLLIQSLEENQQEGLENYLFTDNPRQIDRFEAIFNDLEANVVFSPVTKGIE
                     QGFIDPILRVACYTDHQIFDRYHAYNLRKGFTKDMAINLRMLRELQPGDFVTHIDHGV
                     GKYSGLEKININGHVQESVRLIYKNNDILYVSINSLHKISKYVGKDGTEPVLNRLGSD
                     AWKNLKAKTKKKVKDIAGELIKLYAKRRASTGHSFPPDGYLQNELEASFLYEDTPDQY
                     KTTNEVKADMEKDYPMDRLICGDVGFGKTEIALRAAFKSVTDGKQVAVLVPTTILALQ
                     HYKTFSERLEEFGVTVDYINRFRTNKEKKEIIEKTKNGKIEIIIGTHSLVGKEIEFKD
                     LGLLIVDEEQKFGVSAKEKLRSLKVNVDTLTLTATPIPRTLQFSLMGARDFSVIKTPP
                     PNRQPIHTERWVWDEEKIREAINYEKLRGGQVFFVHNRVKSLEEVAAVLKRMCPDVSI
                     GVAHGQLEPEKLEEILVGFIEKKYDVLACTNIIETGLDIPNANTIIINNAHQFGLSDL
                     HQLRGRVGRSNKKAFCYLMGPPLSGMTSDARKRIQTLEEFTDLGSGFTIAMRDLDIRG
                     AGNLLGAEQSGFIADIGFEIFQKILDEAITELRHGEFKEVFAGQPEETAFVRDVIVET
                     DQEMLIPDEYVSNVQERLALYTELDQLTTEEELKGFADRILDRFGRIPPQVRELMEGL
                     RLRWIAKNFGFERIIIKGGKLRCYFISNPQSAYYETGFFKKLLNSIPEFSLNYGIQLK
                     QSNNALILVKDKIHTLGQTKDILERMYAALQM"
     gene            complement(18230..18769)
                     /locus_tag="PLE23_00065"
     CDS             complement(18230..18769)
                     /locus_tag="PLE23_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81685.1"
                     /translation="MIKYEHSESSFFESNKNYCLKIEEQFKDKNINYSGFCNEYGYEI
                     QSGWQMRDYKFHIKIKKDQSTQNGVILPVDALQQTFNTLKMDGIDKSICLRTGKSALK
                     RFFMKFLYKSVFPTPIYIFTKQKPEADVLSEMSELISANNILKLNVSGGKLFLTATAE
                     IKEPLKLIETLERIISGWH"
     gene            18827..19720
                     /locus_tag="PLE23_00070"
     CDS             18827..19720
                     /locus_tag="PLE23_00070"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81686.1"
                     /translation="MNLYQYPSKILLFGEYSVIAGSEALALPVFNYGASWQKSLVNNT
                     GKDQLKQFAQYLNDPENGFDSFIDIEKLNTQLQEGLYLKSDIPQGYGLGSSGSVCAAV
                     LHRFAKIHPADISTKELQYLFAKMESYFHGTSSGIDPLVAYLQKPILVNADKSLSFPN
                     LPPHTKHSLPEIFLYDSGIARKTEALVKLFREKLENTAFHTAVYSQLVPIVSELIHNW
                     ANGNENVFEHIKKISAWQLENMPEFIPNQVLGVWQKYAQSQSIQFKLCGAGGGGFFLV
                     FKQRHLNLPESLKDKLIALDV"
     gene            complement(19766..19927)
                     /locus_tag="PLE23_00075"
     CDS             complement(19766..19927)
                     /locus_tag="PLE23_00075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81687.1"
                     /translation="MKCKLYDYQGKLVKSVDIAKDGRLDLSDIASGMYHFSIENKYLK
                     LSGNVLVVK"
     gene            complement(19962..21134)
                     /locus_tag="PLE23_00080"
     CDS             complement(19962..21134)
                     /locus_tag="PLE23_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81688.1"
                     /translation="MSSTAAIGTGVSDRITINANGKTDFPNSRVTLNFPTGGSLPNGE
                     MVMTRLYNKPFGQPGVPSLSNHYWILENWGSNKTFNGLTELSFLQSGIEATGSKIYSR
                     PVYDYEQSGWQTKSVNGTQINGGKAGDISFQNQSLIQGANQFVLAGTKVKVVADAGED
                     KKICLGEEVSLIGSGGNVYQWFDASGNLLGTEYILTITPVQTSSYILKVTDLNGEAAS
                     DTVQVTVLPVPDVIIIDQLLTAGTNTNINIPGNYSFHWSNGLTGPDFSTAQAGNYSVI
                     ITNTDACTQIMTFNVNLDSTSLPNIWLVQQDLLGLQNIANSDAYQWYLDDQIIPGATS
                     QTIVPPGTGFYKTYVHYANGFEAITKPFYYKTTSIDVAQGVTLTWSPNPARHSIQI"
     gene            complement(21210..22184)
                     /locus_tag="PLE23_00085"
     CDS             complement(21210..22184)
                     /locus_tag="PLE23_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX81689.1"
                     /translation="MSDWAEYNTGLPVNHASRALKPFYRDGKIRSGSNIGFWEANLYE
                     PSNLLAQPTADKLYTYCARDTFYFDDYSVMKYDGTQSWKWEFTGATTVIGANTRTPKV
                     VYPSAGEYDVKLTISNSSGSNTRFIAKMIKILPSECNVDTIAGKSLKMTGDHKIVSIP
                     AVRKLKNAQSMTVMCWIKLADKQQWFTQLVSNWGSNVGFGFGFAFQGYVPTTNLTFSW
                     KNVPYWQTSSFDLELNKWIHVAMTVEPTKVTLYKDGKPWVYNGDFSNFDLSETPFEIG
                     CPVYGQGGTFNGEMEEFRIYNKAFSQAEIREQMHLIDKNPDSSLVLYY"
     gene            complement(22197..23516)
                     /locus_tag="PLE23_00090"
     CDS             complement(22197..23516)
                     /locus_tag="PLE23_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81690.1"
                     /translation="MLKKNTTKDFYECWKSTNGGSSFTAKTIGWPTGLKYGEGRIAVT
                     PADPSRVYAILLTDQGPRLMCSNNTAEAWTLTATGSTPAFEMDNGQGYYDLDILASPL
                     DANKIIVATTTAFRSVDGGANFTSVGGYYGPFSRHPDIQAMRSQGNDTYIATDGGITW
                     SDDFFAANSEPRINGIFATDLWGFDSGWNEDVLVGGRYHNGNTAYYQAYPEGKFLRMG
                     GAEAATGYVNPVKNRQTFFSDIGGYEIPENFDGKTVNLPLSEWPNESYYYMEFSEMTW
                     SPVCWNEVYIGKGPSIKKSTNNGVSYSTLFTSTDQDAVMEHIRISRSNPNVIYATQRS
                     NTLWDGKIYKSINGGVSWSTLPDLPVSSGQRRVMTITLSGDNENELWVALAYSSNGKK
                     VYYSPNGGNTWLNWTTDKLNDFNLQDIVHQLGTNGGVYVAADNGRIF"
     gene            complement(23616..24092)
                     /locus_tag="PLE23_00095"
     CDS             complement(23616..24092)
                     /locus_tag="PLE23_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81691.1"
                     /translation="MSTDQINHKRKFAEGKKTLSSEQNDIWTPVGPLTTHWKSEDNAD
                     MDACPWQANIYCLEIAPSDPGILYIVTETGSFFRSADKGKNWELRGGSYLLNSKALAI
                     HPNDASTLLLGTNNVVRKSTDGGLSWTNIYTNNDLWCNRIIIHPTKPDNILLCSNK"
     gene            24558..25367
                     /gene="bamD"
                     /locus_tag="PLE23_00100"
     CDS             24558..25367
                     /gene="bamD"
                     /locus_tag="PLE23_00100"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HMX81692.1"
                     /translation="MILRKAGLLLVLVFTLLFAGCSNEFENIRKSGNTELIYKKGLEY
                     YDKEDYLKAQTLFEQVLSSFRGKKESETLYYKYAYTHFYLRNYILASYYFKTAANIFS
                     TGENKEESEYMSAYCNYMISPDYELDQTETLKAIDGFQAFINTYPNSKRVEDANKNID
                     ELRRKLAKKSLAEATLYYNTKQYLSAITSYNNLLNDYPDITDREYIKYMVIKSTYDWA
                     MRSFSFKQTERFNMVIDKYEEFVNKYPKSKYLKDIKTFYRNSKSKLKEINL"
     gene            25364..25708
                     /locus_tag="PLE23_00105"
     CDS             25364..25708
                     /locus_tag="PLE23_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HMX81693.1"
                     /translation="MTDIKTQVQGLDSNVQARDVTSIAAKTGNIYESLNIVAKRANQL
                     AIDIKQELHSKLDEFAVSSDTIEEVQENKEQIEISKFYERLPNPVIIALNEFMTDQIH
                     YHYKNQTEESAE"
     gene            25724..26926
                     /gene="coaBC"
                     /locus_tag="PLE23_00110"
     CDS             25724..26926
                     /gene="coaBC"
                     /locus_tag="PLE23_00110"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HMX81694.1"
                     /translation="MSHLAGKKIILGVCGSIAAYKSAYLVRWFIKKGADVKVIMTVAA
                     KDFITPLTLSTLSTHPVISDIADEESWNNHVELGLWADALVIAPVTATTLSKLANGNC
                     DNMLTATYLSARCPVWVAPAMDLDMWKHPATKRNINLLVQDGVTVIPPAIGELASGLT
                     GEGRMPEPDEIGMLVDKHFSTSQHWSGKKVLITAGPTFETIDPVRFIGNRSSGKMGIA
                     LAENLSRKGAEVTLVLGPTHLLPNCSNIRVVHVESAQEMFEAATVAFENCDLGILAAA
                     VADFRPVTVANSKIKKTDSDLNISLAQTQDIAKYLGSVKKKEQLLVGFALETDNEHEN
                     ARKKIISKNFDLIVLNSLKDKGAGFQHDTNKITILTKDNKQLHFELKDKSEVAEDIVV
                     AIEDYFKK"
     gene            26930..27838
                     /locus_tag="PLE23_00115"
     CDS             26930..27838
                     /locus_tag="PLE23_00115"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HMX81695.1"
                     /translation="MSYRNTFLAFIFILLTTTLHSQELLCKVVVNTPKLQTADPKVFQ
                     TLQTAIRDFMNNTAWTDVSYDNFEKIDVNITLNITEEVNANTFRGDFAIQMVRPVYNS
                     DYKTPVFSYVDKDFAFIYEQYQPIQFSRGVFNDNLSSMLAFYAYYLIALDYDTFSALG
                     GENYYLIAQDIINAIPQSVSGAFKGWKPSDGNRTRYFLLENSLNPGMKAVRQGFYEYY
                     RKGLDLMSQNPETGREGLMKCLEYCDATNRSYPNSSAVQIFCTTKGDEIINIFKAADA
                     AQRAKVIQYMTRMDPANTNKYVQLSY"
     gene            27938..28444
                     /locus_tag="PLE23_00120"
     CDS             27938..28444
                     /locus_tag="PLE23_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81696.1"
                     /translation="MTLRDGFDYAHTNPAWFIFFFVMLPVVALLVGWISKEEGINSPW
                     REIYGALIYLACVPGIFAVTLDIYLFLFERQSIFDSNLVLQVLPIISMVVTLQIIKHN
                     VSLDLIPGFKKLSGLVMIIFFTISIMWIIDRTHIYAIAFLSFQAVLVIFVVLFLVVRY
                     GWYLMVSK"
     gene            28483..29196
                     /locus_tag="PLE23_00125"
     CDS             28483..29196
                     /locus_tag="PLE23_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX81697.1"
                     /translation="MTVYLIHGFGMNNSIWRKITKNIQAENIILTNLPGYDGTDEKYY
                     DNIESLASAYKTLIKRPGIIVGHSMGGYLALQICKLFPEKIRGLMLFHSHPFADSGDK
                     QEGRKKHISFLETHGVAKFYKEIMPNFFHNKANKEIEQLINNGNNMRVSLLVNNLQIM
                     TSRPDMTDFLTKTNIPVGFIIGKYDNLIDGEAWFAQTAFPKKAAVYALKKSAHLGMLE
                     EPRKSTEAINHFIETCSLL"
     gene            complement(29188..29526)
                     /locus_tag="PLE23_00130"
     CDS             complement(29188..29526)
                     /locus_tag="PLE23_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HMX81698.1"
                     /translation="MQLREAQKLVDNWIKKVGVRYFNELTNMAILTEETGEVARLISR
                     IYGEQSFKDPTTDDEAKAMLTDELADVLFVLICIANQTGVDLDEALTNNLKKKTERDI
                     DRHQNNDKLK"
     gene            complement(29527..29979)
                     /gene="dtd"
                     /locus_tag="PLE23_00135"
     CDS             complement(29527..29979)
                     /gene="dtd"
                     /locus_tag="PLE23_00135"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HMX81699.1"
                     /translation="MRAVIQRVNKASVEVDNTIIGKIDEGLLVLIGIENEDSKQDAEW
                     LASKIVKMRVFADNTGLMNLDITDINGELLVISQFTLFASTKKGNRPSFINSAKPDIA
                     IPLYNYFLQFIKNQLNIPVKSGIFGADMKVSLVNNGPVTIIIDTKKKE"
     gene            complement(29989..30642)
                     /locus_tag="PLE23_00140"
     CDS             complement(29989..30642)
                     /locus_tag="PLE23_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - cellular response to DNA damage
                     stimulus [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HMX81700.1"
                     /translation="MCSRLSFVTTSEKIWKDIGIQSEGLLRTSYNIAPTHHTYIIKNS
                     TPDRLDYVTWGIIPQTAQIIKNEGKLALARAETLLTSTSFRIPARQKRCIVVVDSFYD
                     LQEHFGPSQSTAKRYFSKNGELLTLAGVWDSCNIDGYNHLSFSIITQQSEYFPNTRLP
                     LIIHESELRKQWLSNLQINEIEDILLTDHSNMLDSYNVNAAELLAFNDSFQMHLRID"
     gene            31138..32199
                     /locus_tag="PLE23_00145"
     CDS             31138..32199
                     /locus_tag="PLE23_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HMX81701.1"
                     /translation="MSGNRITAVQIWLIVGLFMILMQVFIGGITRLTGSGLSITKWDI
                     VSGTIPPLNENEWNAQFELYQQTPQYHKINKGMSLSEFKFIFFWEYFHRLWARTMGFV
                     FLFPLIFFIFKRKISSLLGKRLFIVFLLAALNASMGWIMVASGLIERPWVNAYKLSFH
                     LILALILFLYLLWVVIQEFRVEKEDSIDVTNRIAYKSFFWILFVQIFLGGMMSGMRAS
                     MIYPTWPKIGDSWIPAVLTDFSHWTYTNFGKYDEHHFLPALVHFLHRNLAYLILCFGL
                     YLFFKNIGRVKSALLKTGLYLQITFMFLQVILGVLTLIHSKGRIPIWYGVFHQLVGFL
                     LISAIVYILSLVHDSQKTV"
     gene            32251..33018
                     /locus_tag="PLE23_00150"
     CDS             32251..33018
                     /locus_tag="PLE23_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX81702.1"
                     /translation="MEYELIEEFGFKYIETNGGKETLVLLHGLLGALSNFEGIMKEFH
                     DRYNVVIPILPIMELPMLKLSVGGLVDHVYDFIRFKNFDKVHVLGNSLGGHISILFAL
                     AHPQHVATITLTGSSGLFESAMGTSFPKRGSYEYIEKKAQETFYDPAVATKELVDEIF
                     STISDRLKALRIVVTSKSAVRQNLEHRLHEIEKPTLLIWGHQDIVTPPFVGEKFHSLI
                     KNSELHFIDKSGHAPMMERPEEFNAILSSFLDRNRLL"
     gene            33160..34971
                     /gene="typA"
                     /locus_tag="PLE23_00155"
     CDS             33160..34971
                     /gene="typA"
                     /locus_tag="PLE23_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMX81703.1"
                     /translation="MQNIRNVAIIAHVDHGKTTLVDKMLLAGKLFKDHEKPGELLLDN
                     NDLERERGITILAKNVAVRYKDYRINIIDTPGHSDFGGEVERVLNMADGVILLVDAFE
                     GPMPQTRFVLQKAIGLGLKPIVVINKVDKENCTPDEVQEQVFDLMCALDATEDQLDFP
                     TLFGSAKNNWLADDWRNPTTDITQLFEAIVKYIPAPIVSAGTPQLMITSLDYSAYIGR
                     IAIGKLTRGTLTSGQNVSLVKRDSSITKTKIKELYYFDGFEKIKTNEILAGDICAIVG
                     LEGFEIGDTVADFENPEALATIAVDEPTMSMLFTINDSPFFGKEGKFVTSRHIKERLE
                     KELEKNLALRVEKTDSADSFMVYGRGVLHLSVLIETMRREGYELQIGQPQVIFKQIDG
                     QKCEPIEELSIDVPEEFSGKAIELATQRKAEMKQMEPKGDRMLLSFHIPSRGIIGLRS
                     NLLTATAGEAIMAHRYIEYQPYKGDITTRINGSLICMETGQAIPYSLNNLKDRGRFFI
                     ESNSDVYEGQVIGENNRSGDLVVNITKTKKLTNMRASGSDDKVKLPPPLIFSLEEALE
                     YIQADEFVEVTPHSIRLRKIYLKEHERKKAGKVVETV"
     gene            35295..>38597
                     /locus_tag="PLE23_00160"
     CDS             35295..>38597
                     /locus_tag="PLE23_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81704.1"
                     /translation="MKKIYICLILLLFASEAFSQTYPPCTNGSQLVCKCNTAPLLCTI
                     DELDGYVLTMNNYQHPGDAPTPLCPGSNTVPNNPTWFAFKAWCTDLTLNCTLTNCTGK
                     GGSNGVQIAIYNQCGSNDAVACNVNDCGNTNDKTLSMSGLQIGKTYYFMIDGCAGSYC
                     TVTIDIIGTCGSETIEDWKGPIMGETEICAPKTETYTVDKLDGATHYIWYLDGAVVKN
                     STSNSFTKNWTTAGTYTLCVDAYNDPCIPVTNPPAQNCITIVVNSANAGTITAAPTPL
                     CPDAPVTYNVTGYETNPNNTEVVFVTNAAGEILEIFPGVTGTWTSSECGTFTFYSYNY
                     VTGSNAYVPVVGGNVSSIDCTANCCDLKSKSVTFQDTQKPVLQNKPPNQTLSCYDLLQ
                     PMQALNYTDNCIPNGSVPGEETGSADLCSGGTITRTWTIEDNCGNKDSHTQTITINAV
                     PAPTWKSTLPQNQTLTCSQIPTTHPNLDYENTGMGACLVSGTVAPVVTGSADLCGGTI
                     TRTWTFTDNCNHTINHEQVLTVTPVPEASFQNPPANMTITCDQIPTSHPSLTYTNGLS
                     GACGISGTSIPTVTGSADLCGGTITRTWTFTDACGRTKTHEQVLTVTPVPQASFQNPP
                     ANLNVDCSQIPTTHPDLSYSNGQTGACGISGSVSPVVTGSADLCGGTITRTWTFTDAC
                     NRTITHVQTITATPVPAAAFQNAPSSQTVDCAQIPTGASDLSYTNGLSGACGINGSVP
                     AVQSGNADLCGGTITYTWNFTDACNRTITHVQTFTANPVPVATFQNAPSSQTVDCAQI
                     PTGAADLTYTNGLSGACGINGSVPAVQSGNADLCGGTITYTWNFTDACNRSISWVQTY
                     NVNPVPVASFQNAPSSQTVECEQIPTSAPALTYTNGLNGACGINGSVSPVESGFANLC
                     GGTKTYTWNFTDACNRTINWVQNYTVNAVPVATFQNPPGDKTVNCSEIPVSVPDLNYT
                     NNRTGLCAITGTVPAVKSGNSDLCGGTTTYTWTYTDACFRTISHAQNYTATPVPVAVF
                     QNPPGDQVVDCENIPASAPSLSFSNGLSGACGINGSVPATLIGSPSVCGGTFGYNWTY
                     TDACFRTISHTQN"
BASE COUNT        11499 a         7609 c         7583 g        11906 t
ORIGIN      
        1 gggaggaagg tgaaaggatg aaggcggaag gcgaaaggat gaaaaaacca tgatacaagg
       61 ctcgatattg ttttcattca gattagattt ttgtaatgat aaattcgaca cgacggtttt
      121 tttcccggag ttcgacagat gcattatcat tgaccggctg tgatgcacca taacctttgg
      181 tcgttaacct tgctccatca ataccggact gtattaaaaa gctctttaca gactcagctc
      241 tttcttgtga caacttgagc aagtcttcta ctttaccgag attatccgta tgtccttcta
      301 ttctgatgca aagagaacta ttattccgca ataattctgc aagtttttcg agttctggct
      361 ttgatttttg gagaatttcg ggttttgatt gcacaaaata aatcggattc atactaatac
      421 ggccgtccac taccttcggg tcgatgtaga aattgatttc aacagaattt gaataaccga
      481 tttgcaaagc tgatacatca atgatatagg gttgtgagag atagttttcg ttaaacagaa
      541 aaatttttat tttgtcattt ttaccagcta ctgtggtaaa ttcaccggtg cttgtttcga
      601 acattttacg ataattatct tctgattcat taccgatgta aactgaagat cttactggtc
      661 tcaatgtttt ggaatcaata acttttccaa aaatgttaat ttttacaggg ttctggtcgc
      721 caatttttgc cctgtaaatg tcggatgaac ctgctctgcg agttgaaaaa taaagatagc
      781 cggtagagga attataatgt ggctgaaaat catctttagc agaattgatg ttttcatgca
      841 atggtttggg tgtagtccat tgctcccaat cagtgtcggg tttggtagcc atatatatat
      901 cgagtccgcc atagccaccg ggtctgctgc tggcaaaaaa caaggttcgc ccatcttctg
      961 tgaggtatgg tgatatttca ttggcagcag aatttacggc cttcaaatta acgggttccg
     1021 accacaggct atctttaata tgctgacaga tatacagatc attctcaccc aaagaatcat
     1081 cacgcttaag agacaacaca gctacctttc catctgcgct catcgtaaag cttacaatat
     1141 cactgttgtt gaagtaatta tctatataca atggtttggg gtagtaccac tcggtatcat
     1201 tttttttgtc tatggtcgaa aaacccttga gcattccacc accttttgga aactggttga
     1261 tgataacgaa acggttatta acaggagtga gcgaactgat gctgttgggc aatgcattat
     1321 tgagtggtgg cccgggatga tagactttat caaatttctt cccatcggct gatgtggcta
     1381 tccatacatc ctggttgaag gatgacctgt gagggttgcg cacttcattg tctcccaact
     1441 gtcggtagat gtcggccaat aactccatgt atctggtttc aggattggtt tcatagaggt
     1501 ttcgatcatt tagctccaag tatttttcaa agtcgggtga gcctgatctt gtaaagtata
     1561 atatagaacc atcagggctc agaaccggag aaatttcctg atagatatct gtattgacag
     1621 tttcgccaag tttttcgatt gcaacattct gggcattaac aacaagagtt gaatctgcaa
     1681 cataaaggat tataatacac gtaattctta tacaaaaaag cttgttcagc aatggaatat
     1741 caaattttag cacaaagttg ctaaatattt cttgtactgt gattgtttca tgagaagttt
     1801 aataattgat atacttttgt ggtcaaatta aattcatcac gatggatttt ctcaaaaaaa
     1861 tacttgatac acacttattt tctactgatg gctataagct ttctttgcag gacttgcttt
     1921 tgttcactgc catttttata gcttttatat tcatgctgcg ttttgtaatg cgtaggttac
     1981 taccccggtt ttttaaaatt tatgacgtag gtaatgcggg caaatcaaaa attaagtcag
     2041 ccacatttgt agtactcagt ctcattttca tacttgcagc actttggagt ctgcatcttg
     2101 atgctgttct ttattcttca gactatatta ccattaaagt ctcaaacatt ctccaggcta
     2161 tacttgtcat caggcttgca aggatattag acaacatcat taataaaata aaaaataaaa
     2221 accaagacga agaattaagg gttactaccc caggcggaga tacagttgaa aaaaatcagg
     2281 ctcacatcat tcaatggctt gtgtatctga tagcagcact cattctggtc aatttttttg
     2341 aaatagataa acccttggtc accatcaaag gtttttcact caagatttca tccatcttga
     2401 tcgtaattat cattctgttc ggctcacgtt tgttagcctg gtttttgacc aatattttac
     2461 tccaacccta ctatgcaaga aacaaaatag aacggggcgc tcaatatgca gtcaaccagc
     2521 taatgtcgta catcatctat cttgtagcat tcttttttgc attacatgtt attgatgtac
     2581 agatgagcct tatacttggt ggtgcagcag cactgctggt aggtgtcgga cttggattac
     2641 aacagacttt taatgatttc ttttcaggta tcacactact caccgaacga agcatagaca
     2701 taggcgatgt agtcaatata aataatactg taggcacggt aagacacata ggtattcgga
     2761 cttccaagat agagacacgt ggcaacctga ctatgatagt acccaactcc aaacttacat
     2821 ctgacaatgt tatcaactgg actcacttta ataatctggc aagactggag gtaaaagttc
     2881 cggtaccgtt ttctgctgat cccgaacttg tcagaaccat acttctgaaa gctatcttac
     2941 ccgatccaag aatacaaaag tatcccgagc cgtctgttgt atttaatgac tttggccaat
     3001 atgcccacga tttcatcctg atggcatggt caacggatgt attgaacttc gaagacatca
     3061 aaagtgaact caggttcgaa atcaaaagat tattcaaaga aaatggaatt gaaattccat
     3121 atccgataca attttacgcc aatatacctg aatcctaaag caatgatttg attctttgat
     3181 agttagtttg tggtttatca gaatagtttg aacagtgtag ttttgctgcc tgttcttcaa
     3241 tcgcctttac acgattgcct gggtcagggt gtgtacttaa aaattgtggc gtagaatttt
     3301 ttccttttat ttttttaaaa aaacctgctg caccagcccc gtcataacca ctattgcaca
     3361 gataatatac actgtacttg tctgcttctt tttcgtggtc acgactaaag ctcaacgatg
     3421 ctagtgtggt agtaatgttt gcaatggcgg ctttattctt attattgcca gccaatactt
     3481 cacctactac tgccaaaggc ataaccttgg tgagctgtct ggtagagtgc cgcatatcag
     3541 cgtgtgctat ttcgtgtccg agtacaccag ctagctgatc ttcggtatca agatatttta
     3601 taaggcctgt gtaaacataa atatatcctc ccggagtgca aaatgcattc aataccttgt
     3661 cgtctttgat tattttcact tcccaggcga aatttttctt gtttttgaca cgacccgaat
     3721 taagaatgtt atcttttatt cctcgaataa atctgtaaac ttcttcattg ccctgctcgg
     3781 gcaataacgg atattcgttt ggcttagagg caatctcctg cgataccgac agtcctaatt
     3841 ttttatcatc ttcaattgaa tacatgttcc actttttcca gctctgacat gaaagattgg
     3901 aaaccgacgt aaggaatatt aatgctaata ctgataaatt tttcatttca attcttttta
     3961 ttaatacgaa tatataacaa aaagtaactc actacatacc aacattaata taattataac
     4021 atcgcaccga ccccgaatga aatatattct gctgcaaact tatgcgcctt aagctgcaca
     4081 ccaccataga accgaactct tgttttatac atctcaggga aataatacct cgacgtgagt
     4141 tttgtaaaaa tcttcccgaa acggtagtca aaatcggtat ctaggtaggt acctgcctgc
     4201 aacataatac taaaaggcca aaagaaaaat tcttcagcta caaatactag atggcgggta
     4261 gctgcctgtg aagctatttt ttctgaagat gcttccgtag aacgcagtaa aaagtaataa
     4321 tcagcactgt ggtattctat ctccgttccg agtaacaact gattggcttt tgatagtctg
     4381 tatattccac cgagcgacgc tatataaaca ggatatttcg gacctcccgg caatgattgt
     4441 tcgacaaatg caagacctcc atgcaacgaa agtccgaacc tgtgtgtcgg gtctggatcg
     4501 acatcgctta cttcatattc tgaaggtact actacattag gtgtccactg gagaccgagc
     4561 tgagcggtca caagattaat tcccagattg ggtaactttg atgaaccatt cgaaaaatgt
     4621 gttagtccaa agcctcccaa catttttact tcatttgaaa ttcttctgtg ccagttaaac
     4681 tgcaacaccg taatgttgtt aatgtgcgaa ccaatggctg tttggagcgg attgtctgtg
     4741 tagtcaaatt tttttgtcag gtatgccaca cctgttccaa ccaaaaacct aaactgggtt
     4801 ttgtctgctc cgccaagctt gagtgaaata ttaggcaata atgaaaatgc actacccaag
     4861 atatgatcgt cacctaggtg aaaataccca aagctgacac ccaactcagg gtacttttgc
     4921 cagctgttcc agtattggct gccatacgta cggtaaacaa cattggctgc cacacctaca
     4981 gaataatcag gtacccgaaa caatattttt ggcgaatgtt tgagcatttt accgtacaca
     5041 agctgtggtg ttatggcaat attgtctgtt ttttgggcaa atgatacaca ttttaaccat
     5101 accaaaaggc aaataacaac tacagacctc atacctgatg gctgtgttct ttcaggaata
     5161 tccttgtagc ttctatatct ttactcaatt gtctgtcatt atccatccta ctcacctgct
     5221 ctcggaacaa atcatgcaaa tattcaagtg cctctgagct acgtgccggc cgtctgaact
     5281 caattgcctg acatgcatta agccattcga tagccagtat ggtccatata ttttctacta
     5341 tgcgatagag tttggtagct gcattggccg ccatacttac atggtcttcc tgcccattgg
     5401 acgaaacaat agaatcaact gatgctggag tgcaaagttg tttattttgg ctgacaatag
     5461 atgcagctgt atattgcgga atcataaacc cggagttgag tccggcattt ttgacaagaa
     5521 aaaccggcag attcctcaga ccagaaatga gctggtaggt tcttctctca gaaatgctgc
     5581 ctaactcgga taatgatatg gcaagaaagt ctagcacaag cgctataggt tgtgcatgga
     5641 aattgccacc cgacaatatc tgatcttctt ctacgaatat gttcggatta tcagtcactg
     5701 catttacttc attctcaagt acactcatca catactgtat ggtgtcataa gtagcgccat
     5761 gtacctgtgg tatgcacctg aaggcataag gatcctgcac cgccacacgg tcggtctcca
     5821 gcagtgggct accttcgagc cattttctta tttgccctgc tactgtttgc tgcccgggct
     5881 ggtttctgat tttatgtagt ttgtcgtccc aaggtgtagc agagcaaagg aatgcttcgg
     5941 ctgacattgc accgacacag ttggcccatc gcataatttc ctgagcttct atgatagcat
     6001 atacaccata gccggtagaa aattgagttc cattcagcaa tgccaaccct tcttttgcac
     6061 ccaaatcgag tggatttaag ccaaacattg ccaatacttc ttcggtcggt ctgattttcc
     6121 cttgatatat gacattccct tctcctataa gcggtaaact cagatgtgcc aaaggtgcca
     6181 gatcaccgga tgcacccaat gaaccctgtt gaaaaataac cggcagtata tcatggttat
     6241 agaaaaatat aagcctgtca ataacttcct ttgatacacc cgaatgtccg taggcaaggc
     6301 tccttatctt tagcaacagg attattttta caacgttggc aggaactgaa tcacccgtgc
     6361 cacatgcatg tgacaatacc agattttttt gaaggtcgcg cagttggtcc ttgccaatga
     6421 catggttaca caatgaacca aagcctgtgt tgataccata aaaagcctga ttgccactgg
     6481 atattttact ctgcaggaag ttatgacatt tttgtacagc ctcaattgct tcttcgctta
     6541 gctttatttc acattcacga tcaataatgg atgctatatc attgatggtg taagattcta
     6601 gcgttaatag gtgttgatct cgcattgtca tttcttaatt aatggcaaaa ataagctacc
     6661 taagcggtaa ttttatccaa taaagcctta ttgcatcata ttgttgtcgg ataaaagcta
     6721 ttaatggctt atttttacac agatttttca aaagcatggc agaaaaccta caagataaaa
     6781 atattattgc ttctgatccg acggctgaat cacgcaaaca ggatcacatc gatcttgcat
     6841 ttaggtcgca ggtggatgtc ttacgtgctg atggcagatt ttactatgaa cccttgcttg
     6901 caggtttctc tgacaaggat atttacccac agacacagtt acttggcaaa acgtttcgtt
     6961 acccgatgtg gatatcgagt atgaccggtg gtaataaaat ggccggaaaa ataaatgaaa
     7021 atctggcacg tgtatgtaat gatttcggac tgggattcgg gcttgggtca tgccggcaat
     7081 tacttactga ccacacttat cttaaagact tcgatgtaag aaaatatctg ggtgacagac
     7141 cattttatgc caatcttggt gtggcacaga tagaagagct actcataaac aacaaagttg
     7201 atgaaataat caggcttgta gatttgctaa aggcagacgg gcttatagtg catgtcaatc
     7261 cgctacagga atggtcacaa cctgaaggca acttgtacac acaaaggcca attgaaacaa
     7321 tcgaaaaact gcttgaaaag agcaagctca acatcatcgt caaggaagta ggccagggaa
     7381 tgggcaaaaa gagtctcgaa gcattggtga aacttccact tgcagccata gattttgcgg
     7441 cttttggagg tacaaatttt tcaaaacttg aattgtaccg aaaccctgaa aaacagaaag
     7501 cccatgaagt aatgtcactg gtaggccaca ctgctgaaga aatggtcagt atggtaaacg
     7561 aaatactcag ccattcaacc gatatgatcc gctgtcgcga atttatcgtt tctggtggca
     7621 taaggagtta tcttgacgga tattatctga cccgaaatat aaatgctgcg tccatatatg
     7681 ggcaggcttc cggctttttg aagcatgcta tggaagatta cgaccaactt taccgttatg
     7741 tgaaggcaca aactgaaggt cttgaactgg caaacacttt tttaaccatc aaagaataaa
     7801 cgtagaaatt gaaagacgac aagcagataa atggattctc aaaacgctca aaagctgaaa
     7861 agcttcaatg ggtgatagag aattttttca atggccatga gtcagctgcc ggagagctca
     7921 ggagtttttg gttggatgac gagacgcaaa acatatttga tgccttcagt gaaaactcca
     7981 ttagcaattt tatactaccc tacggtgtgg cacccaatat gctcatcaac ggaaaaactt
     8041 actgtgtacc tatggttaca gaagaaagtt cggtcgtagc cgcggctgca gctgccgcaa
     8101 aatattggtc aacccgcggc ggaataaaag caaccgtagt caataccaaa aaaatcggac
     8161 aggttcactt caaatggtca ggtcaaactg aaaaactcat tagcgtcttt ccggagttaa
     8221 aaaaagagct cattataaca gcctcagcta tcactaccaa tatggataaa cgtggtggtg
     8281 ggatccttga catcgaattg aaagatcttt ctgataaaga accagattac taccagttat
     8341 tcactcactt cgacacttgt gattcgatgg gtgccaattt tatcaattct gtcctcgaac
     8401 agtttggccg tacattacgg ttattcattc aaacgcatcc agcatttgat gaaaccgacc
     8461 gtgaagtgga tattattatg tgtatattgt caaactacac gcctgaatgc cttgtaaaag
     8521 cagaagtaag ctgtaccatc gacgagctag gtgatgaatg cctcgaaatg gacgctgtcg
     8581 gctttgctga aaggtttgcc acggctatcc gtattgccga aattgacagt taccgggcta
     8641 ctactcataa caaaggcata tttaacggca ttgatgctgt cgtaatagct accggaaatg
     8701 atttcagagc aattgaagca tctgggcaca catacgctgc acgttcgggt atttacaaga
     8761 gccttagcaa atgtaccatt aaagataata ttttcagctt caacctcgaa ataccactcg
     8821 ccattggcac cataggcgga ttgacaaaac tccatcccat cgcaagacat tcgctagaga
     8881 tgctcggaca tccttctgct cctgagttga tgaccatcat tgcagctacc ggattgatgc
     8941 agaattttgc tgccatacgg tcgttggtga ctacaggtat tcagaaaggt cacatgaaaa
     9001 tgcaccttat gaatatattg aatcatctga gtgctacaga acaagagata gagcaagctt
     9061 ttgaatattt ttcagataaa gtgatttctt ttacctctgt cagagaattg atcacagaaa
     9121 taagagaaag agaacataag attattacag attaaaattt ttataccatc aaaaagaatt
     9181 tttcagcaaa cggcaaacta ctactgacag cagaatatct cgttctggaa ggtgcacttg
     9241 ctcttgccct ccctgtacgc ttcggtcaga aaatgtcagt aaccgaaaac agctcttcag
     9301 aaataaagtg gagcagtctt gaccacaaag atgagctgtg gttttctgca agcttttcat
     9361 tgtatgattt taaagccacc gaaacaaccg atgaagagat agctgctcgt atcacaaaat
     9421 tgcttaaagg gtgtgcccgg caagattcag agtttctctc gcaatggaaa ggtcagaaag
     9481 tagaaaccaa actcgaattc gaccgcaaat gggggctcgg taccagctct acactggtgt
     9541 actgcattgc agaatgggcc gacgtcaacc cataccagct gctgatggat acctttggcg
     9601 gtagtgggta cgacatagcc tgcgccggtg cagatggtcc ggtgttgtac agtttacgcg
     9661 attatgctat ttctgttgaa gaatctcttt tttatccatc attcaggaac aatctttttt
     9721 ttatttattc ggggcgtaaa caaaactcac aagcagcggt taaagatttt caaagactaa
     9781 agaatttttc aaccaaagac atcgacactg ctagcgaact gaccgaatcc tttgaaaaat
     9841 gtacaaacct gcatgatttt aaaaaactga taacagagca cgaattgctt atttcaaaaa
     9901 tattaaaaac ttcacccatg aaaaacgaat attttcatga ttttgaagga gaaataaaat
     9961 cactcggagc ttggggtgga gattttatac ttgcagccac agaaagtgac aaaaaatatg
    10021 tcactgaata ttttacagga aaaggcctaa cagatttttt cagttacgat gatctgattt
    10081 taaaaagaaa ataaaaatgc ctcctaagtg gattgaagca aaacttacag aaattaaacc
    10141 tgtaactgat aaaatacgaa atttttacct tactcttaat gatgaagatc ggtttggctt
    10201 tttaccagga caattcgtta ccttcgatct gcccgtttca gaaaaaagac tacaacgctg
    10261 gaaaagttat tcgatagcca ataggcctga tggtagcaat acgctcgaat tttgtattgt
    10321 tcaccttgat ggcgggctcg ggtcaggtta tctgttcaat gaagtacagc cgggttcatt
    10381 actcagattt aaggggcctg acggtatatt taccctgaaa gaacccctag attatgatct
    10441 ggtaatgctt tgtaccggca caggtgtagc accgtttaaa agtatgcttg atcacatttt
    10501 tcaccataat attcctcacc gtcaacttca cctcatattt ggtacacgaa aagaagaaga
    10561 cattctctac agaaaggaat ttgaagaata tgccgcggtt catcccgagt tcagatatga
    10621 tgttgtcctg tccagacaag aaaactggcc cggatacaaa gggcatatac accaagtgta
    10681 tctgaaagaa tacgccgaaa caagaccgga tgtgagattt atgatctgtg gttggagcca
    10741 aatgtctgat caggctactg aatacctgac gcaaaaatgc ggttatgaaa aatcacaggt
    10801 acactgcgaa ttgtacggat aagattggtt gttgaacaat tttgctgtaa ttttgtagaa
    10861 taattctaaa taagtaaaaa tggaacctac gattaagtca ccggtaatgt tatatactga
    10921 acagacccct aacccggagt cgctcaaatt cgttaccaac agaatgcttt atcacggcac
    10981 tgctgatttt aaagataaag aaagcgcaca ggagtggtcg ccaatggctc ttgctctgta
    11041 tgatttgcct tatgtaagag gcgtatatat caataataat ttcgtaaccg tcaccaaaga
    11101 aataaactac aactgggacg acatcatgct tcgcatcaag gattttatta aaaaatatgt
    11161 ggaaagcgaa gcagtagtca tcaatgacgg ctatgcagaa gcaatggaaa aacaagcaaa
    11221 aatagagcag gaaacacctt atgaaggtga cgaagccgag cttgttaaga aaatagtcaa
    11281 catcattgat acctatgtca aacctgcagt tgggctcgat ggtggtaata ttgaatttaa
    11341 aaaatatgaa gacggagtag tttatgttat aatgcagggt tcttgcagcg gctgcccatc
    11401 ttcaacgctt acccttaagg ccggtataga aggtatgctc aaaagaatgg tacctcaagt
    11461 aagagaagtc gttcaggaaa tgggttaatg gccggtatgg acaggtttac taacgcagga
    11521 gtatgagttc tccacgatat aaagccgttt cgccgttatc aaactcaaga acagcccagt
    11581 aaacaaagac tcccgggtcg agtaacttgc cgcgtgtttt accgttccac cccagcgacg
    11641 gattgttggg ttcaatattc tttgctgaga acaggtgttc gccccaccta gaatatatgt
    11701 tcaactcttt gatgctaaca aggtgatggt caccgtaaac ggtaaaataa tcgttgatac
    11761 cgtcaagatc gggtgaaaac acattcggaa tataaacctt gatatctggt attacgctta
    11821 gtaagatgct gtctgatgcc tgacacctat gttgattttc ggcatacagg aatattttct
    11881 ggtcgtgatt aatgattaat gaattggtct tgcaagcgat acaagagaat acgcttgcag
    11941 gtgcccattg tatttgataa gctgtgtcag aataaaccgg tatcagatct atcaatgttc
    12001 cttcctttac ttttttgtcg ggtccgagtt cgaccgtaaa tgtgctgcct ttgtcaattg
    12061 ttaggttttt tatttctgcc gtacatccct gattgtcttt tatgtacagt gtataatcgc
    12121 caggtgcaag ttttgagaat tcattttccg gaaaataatc gataccattt atactatatt
    12181 gataaggtgg aagtccgttg tgcactgagt ctataacaat acttccatcg tttgagtcct
    12241 tgcatttttc gggttttact aactggaaaa catcaagagg ctttggtata gtctctacaa
    12301 cgatgatact gtcacagccc gagatagata gtaacttggt tgtatcggta gcaatattct
    12361 gcgctgtaca ggagtaattc tgttttttaa tgaaatatga aggcaatagc gaagtattgg
    12421 ttatcacaat gctgtcacat tccgctgttt tgataatctg tatgctgatg cctgccaaag
    12481 ttggatcaca agtagtggca cttacatagg tagtgtccgg taatatcaag gtagaggtga
    12541 gtatgacaat actgtcgcag ccgaactgat ttgtatattt taactcaaaa acacctgcat
    12601 cgctcttttg acaagtgttt ttatactgct taactgtatc agaaggcaat aaatacctgg
    12661 taacaaacac caggctatcg caacccaggc tgtttttgta gttgtattgt gtagtcccgg
    12721 ttagtttagg atcgcaggtg ccttgttgct gattgacaga atcaggctta tggtatatgt
    12781 acttaataaa aacaaaactg tcacattccg ctgttttata tatgatcgta tctaagtaat
    12841 cggggtcatc accacattga tgaacaatga gctcacttga taaataacct gaaaatttca
    12901 attctgtaat aactatactg tcacagccat actgattcag gtattgcgtt gtatcaattc
    12961 cggctttagt agtgtcacaa gtatttttgc tgaaataaac cgtatctttt agtaatggaa
    13021 tatttttaat caacacaaga ctatcgcaat atttgccttg caagcttaaa gtatctacgg
    13081 agaagttatt gattgaacag ctttggtttt caatgatggt cgtgtctggt ttgttgtact
    13141 tgatttggtg aatgacaata ctgtcacacc ccatgctgtt ttgtaatatt aaagtatctg
    13201 acagggcttt actcttgata caggtcgttc cgaatatttt aagcgtatct gcaagtacaa
    13261 gtaaggtagt cgttattaca attgaatcac agccgttaac attatatata agagtatcaa
    13321 tacctgcctg tgacgagtcg caagtggttt tttgaagatt gactgtttgt ccacccggct
    13381 ttatattaaa tgtggcagag aatgtagtaa aattgccgca ttcgtcagtg gctgtaaact
    13441 gaatggtgta gagggttttt gtataattgt tgtcaaaatt gttggaccaa gtgaccgaac
    13501 tgcaattatc actcactgtg gcattagcat gattgttgag ccattgggtg agttttgcat
    13561 aagggtcagc atcacagatg agttcgatgt tttcaggttg tttggtgatg accggatcct
    13621 cggtatcttc tacgatgaat ttagctgttt tggtattttc attaccacag ttgtcagata
    13681 ctataaaact cacttccgtc tcactacatg gaaactctgg ttcattttgg tattcgtagc
    13741 tccacagcac atttccttca caattatcag ttgctgttgc tccgccattt ccattcagcc
    13801 attgattaaa ttctgcctgt atattcaatg aacaatctac gtatatatcc tgcggttcat
    13861 ctacgaatgc gggtgcttcg ttatcttcta taaatacttg ttgtactacg ctagatgttg
    13921 ccccacagct attcgtagct gtccaggtac gtgttatagt ctgattgcat ggtgtacccg
    13981 tggtgctttc actataatca atacttggat tattatcaca atcatcctga accgtaagca
    14041 taggcggagg tggaacatct ttgagacatt gaatgccact gatatcatcg ggcatatctg
    14101 taaatgctgg aatgctcttt ggtgggcaac tgtacatggt gatatcatcg agcaatactc
    14161 caccattgac taccggacca gtacctgtaa atttgagatc ggcagtagaa gcaagagccg
    14221 taaaggtaaa acattttctg agccatccgt ttgcaccgtt gttggtagct gtccatgttt
    14281 ggtcaagcca tgcaccatct gctacctgta tctggcaatt ggcggttggt gtgatagcat
    14341 ttttggcata ccaaagtaca atggtatatt cataatcaac atttaagcca ttcagtatag
    14401 tggaaaacga tcccggtgta ttaccatgaa ggtcaataaa ctgagttgca ccatttggat
    14461 tgccggtcgc ccaattcaga taattaggtc caagcaggtc tattgatcct gagttgacgg
    14521 tccaactact atacatttgc cctgcaaaat acacaataat cgggttggca tacggtgggt
    14581 cttcaaagtt gccacccggt actccaactt cgatgggggt accacaacaa cactgtacac
    14641 agtcaggtac ttgagcctga aatatagctg agccaaatac aaaaaatgtc aacaataata
    14701 gcttgttcac cttcaatcaa tactggatgt taaaacgcaa ttacaatata cgccaacttg
    14761 tggttattta tcaaattttt ttctggtttt ggactttgta ttcacatttg caaagctgcg
    14821 tacattcttt ccaatatatc tttggtctgc ccaagtgtat gtattttgtc tttaacgagt
    14881 attaaggcat tgttcgattg ttttagctga attccataat tgagtgaaaa ctctggtatg
    14941 ctgtttaaca gttttttgaa aaaacctgtt tcgtaatatg ctgattgtgg attggaaatg
    15001 aagtagcatc tcaatttgcc tcctttgatg atgattcttt caaacccaaa atttttagct
    15061 atccatctca gtctcaggcc ttccatcaat tctctcacct gcggtggaat tcttccaaaa
    15121 cggtccagta ttctatctgc aaatcctttg agttcttctt cggtggtgag ttggtcaagt
    15181 tcggtataga gagcaaggcg ttcttgtacg ttgctcacgt attcatccgg tatgagcatt
    15241 tcctggtcgg tttctactat tacatctctg acaaatgctg tttcctccgg ttggccggca
    15301 aagacttctt taaattcgcc atgtcttagt tctgtaatgg cttcatccag tattttctga
    15361 aatatctcaa aaccgatgtc tgctataaat ccgctttgtt cggcgccgag cagattgcct
    15421 gcaccgcgga tgtcaaggtc gcgcatggca atggtaaatc cgctgccaag gtcggtaaat
    15481 tcttcgagtg tttggatgcg tttgcgtgca tcactggtca taccggacaa tggtggcccc
    15541 attaggtagc agaatgcttt tttattcgat ctgccaactc gccctctgag ttggtgcagg
    15601 tcgctcaggc caaactgatg agcattattg atgataatgg tgttggcatt tggtatatcc
    15661 agacctgttt cgatgatgtt ggtacatgct agtacatcgt attttttctc tataaagcct
    15721 actagtattt cttcaagttt ttcgggttcg agctggccgt gtgccacccc tatcgataca
    15781 tccggacaca tacgtttgag tacggcagct acttcttcga ggctctttac acggttgtgt
    15841 acaaaaaata cctgaccgcc acgtaatttt tcataattga tggcttcgcg tattttttct
    15901 tcgtcccata cccaacgttc ggtatgtatc ggttggcggt tgggtggcgg agttttgatg
    15961 actgaaaaat cccttgcacc catcagagaa aactgcaaag tacgtggtat aggtgtagca
    16021 gttaatgtga gcgtatcaac gttgaccttc aatgaccgca gtttttcctt ggcagataca
    16081 ccgaactttt gctcttcatc aactatgagc aacccgagat ctttaaactc tatctcctta
    16141 ccaacaaggc tgtgtgtgcc tataatgatt tctattttgc cgttctttgt tttttcaata
    16201 atttctttct tctctttgtt ggttctgaac ctattgatat agtcaacggt cacaccaaat
    16261 tcttcgagcc tttcggagaa tgtcttatag tgttgcaatg ccaggatagt ggtaggcaca
    16321 agcactgcta cttgcttgcc atccgtcacc gacttgaatg ctgctctaag tgctatctcg
    16381 gtttttccga atcctacatc tccacatatc agcctgtcca tcggataatc cttttccata
    16441 tctgccttta cttcgttggt tgtcttatac tggtcaggag tatcttcata aaggaagctg
    16501 gcctcaagtt cgttttgcag gtatccgtcc ggaggaaaac tgtgacctgt tgacgccctt
    16561 cgtttagcat aaagtttgat gagttcgccg gctatatctt ttactttctt ttttgtttta
    16621 gccttgaggt ttttccatgc atcagaaccc aatcgattca gcacaggttc ggtaccatcc
    16681 ttgcctacat attttgatat tttatgcaac gagttgatac tcacatataa tatatcatta
    16741 tttttataaa tgagtcttac tgactcctgc acatgcccgt ttatatttat tttttcaagt
    16801 ccagaatact ttcccacacc gtggtctata tgtgtgacaa aatctcctgg ctgaagttcg
    16861 cgcaacatcc gcaagttgat ggccatgtct ttggtaaagc ccttgcgaag gttgtatgcg
    16921 tgataacggt cgaatatctg atggtctgta tagcaagcca cccttaatat tgggtcgatg
    16981 aacccctgct cgattccttt agtgactgga gaaaaaacca catttgcttc aaggtcattg
    17041 aatatagcct caaatcggtc aatctgcctt ggattgtctg taaacaaata attctctaaa
    17101 ccttcctgct ggttttcttc caatgattgg ataagtaatt tgaaattctt attgaaggat
    17161 ggttgtggtt ttataccaaa taaaatattg tcaccaccag cggatagacg accggcgtaa
    17221 ccaattcttg taaattgctg tgaagcagaa atgacatcac cggggcgtat gaacgagcgg
    17281 tcacgaatga agtccgtttt ttcatcatca tccatgaatc tgatcttttc accgatgttt
    17341 tcagacgctt caaaacaatt ttgaagaaca tcaactaagt ttttttcgtc agctatataa
    17401 agaatagcat tttttgccaa cagatcaaaa aaactctgct tctggttatg ctcgaatttt
    17461 ttacctacat gtggtactat cacgaccttg cttaccgttt tctgcgaaag ctgcgttacc
    17521 ggatcaaaca aacggatgga ctctacatca tcaccgtaca actcaacacg gtatggccat
    17581 tcattaccaa acgaataaat atccaatata cctccacgta ctgaaaactg gccaggttca
    17641 aaaacgaaat ctgttcgctc aaaaccatat tcgcttagta tttcaattaa aaatccggca
    17701 tcaaaaggtt ctgactttgt aatgataatt ctgtggtctt tgagtgaatc gggcgacacc
    17761 accttttcaa acaaagcctc tggatatgtg atgatgatgc tttggtcatg gtcagactcg
    17821 gagatatagt tgactacttc tgtacgttgc aatacctggg tggaagatat ctgatcaaaa
    17881 tttcccggtt ttttgaaaga atctggcaga taatgcactt tggtgagtgt aaatatatta
    17941 tccagattgt tgaacatgta agccgcttcc tcctggttgt tggcaataat gaccatctgc
    18001 cgtggattgc tcatccaact gcctgcgatg acaaacgcca ccgacgaacc tgcaagccct
    18061 tcaagcgtta tggcttgcgg tgctgaagct gttaataatg aagcgagttt taatattcta
    18121 ctgtcctggc tgtatttctc tagcagtttt gtaagatttt gcacggcaca aatatatgca
    18181 catacaaagt tttaagctcc attttgtttt tatccttacg gaaaattttt tagtgccaac
    18241 cacttatgat ccgttccagt gtttctatca gcttcaatgg ttcttttatc tcagctgttg
    18301 ctgtaagaaa caatttgcct ccagaaacat ttagttttaa aatattattg gcagatatta
    18361 attctgacat ctccgaaaga acatcggctt ctggcttctg ttttgtaaaa atgtaaatag
    18421 gtgtgggaaa aacagatttg tataaaaatt tcataaaaaa ccttttaagt gcagacttgc
    18481 ctgtcctgag gcaaatgctt ttatcaatac catccatctt taatgtattg aaggtttgtt
    18541 gcaatgcatc aaccggcagg ataactccgt tctgtgtact ttgatctttt tttattttaa
    18601 tgtgaaactt ataatcacgc atctgccaac ctgactggat ttcataacca tactcattgc
    18661 agaagccgga ataattaatg tttttatctt tgaactgttc ttcaattttc agacagtaat
    18721 tcttattgct ttcaaaaaag gaagattcgg aatgttcgta ttttatcata gctttatatt
    18781 tttattatta attgttgttg gtttcataat attaacaaaa gattcagtga atttgtatca
    18841 atacccttca aaaatattat tattcggtga atattcggtc atcgcgggct ctgaagcact
    18901 tgcactacct gtctttaatt acggagcatc ttggcaaaaa tcgcttgtga ataatacagg
    18961 aaaagaccaa ctgaagcagt ttgctcaata tctgaacgat cctgaaaatg gttttgattc
    19021 ctttattgat attgaaaaat taaatactca actccaagag ggattatatc tcaagtccga
    19081 tataccgcag ggttatggtc ttggcagttc gggctcagta tgtgcagctg tccttcatcg
    19141 ttttgcaaag attcacccgg cagacatttc aactaaagag ctacaatact tattcgccaa
    19201 aatggaaagt tactttcacg gtacaagttc gggaattgac ccacttgtgg cctatttaca
    19261 aaaacccatt ctggtcaatg cagataagtc attgagtttt cctaacttac ctccgcatac
    19321 aaaacattct ctgcccgaaa tctttttgta cgacagtggc attgcaagga aaaccgaagc
    19381 tctcgtcaag ttgttcaggg aaaaacttga aaataccgca ttccacacag cagtttactc
    19441 tcaactcgta cccatcgtga gtgaacttat tcataactgg gcaaacggta acgaaaatgt
    19501 attcgaacac ataaaaaaaa tatctgcctg gcaattagaa aatatgcctg aattcattcc
    19561 taaccaagtg ctgggtgtat ggcaaaaata tgctcaatca caatctatac aattcaaact
    19621 ctgcggtgcc ggtggtggtg gttttttcct ggtgttcaaa cagagacatt taaacttacc
    19681 cgaatcatta aaagacaaac tgattgctct tgatgtttga tcactcacca aaataacatc
    19741 taaaaacact atcgtaaata ttcaattact tgactacaag cacattacca cttagtttta
    19801 gatatttatt ttcgatacta aaatgataca tacctgaagc aatatcgcta aggtcaagcc
    19861 taccgtcttt tgcgatatca accgatttta caagtttgcc ctggtagtca tatagcttgc
    19921 atttcatcaa agacaaatct atgccattcg gattatcaaa actaaatttg tatggaatgc
    19981 cgcgccggat taggtgacca tgtaagtgtc actccttgcg ctacatcaat agaagtggtt
    20041 ttgtagtaaa atggctttgt aatggcttca aatccattgg cataatgtac gtaggtttta
    20101 taaaatccag ttcccggcgg aacgattgtc tgtgaagtag ccccgggtat tatctgatca
    20161 tcaagatacc actggtaggc atcactgttg gctatgtttt gcaaaccaag caggtcttgc
    20221 tgtacaagcc agatattcgg cagacttgta ctgtcgaggt ttacattaaa tgtcatgatt
    20281 tgagtacatg cgtctgtatt ggtaataatc acagaatagt ttccggcctg agctgtagaa
    20341 aaatcaggtc ctgtgagtcc attgctccag tggaatgaat agttgcccgg tatattaata
    20401 ttggtattcg taccagctgt caagagctga tcaataatga tgacatcagg tacaggcaat
    20461 actgtaacct gtactgtatc actggccgct tcaccattga ggtctgtaac tttgagaata
    20521 taggatgatg tctgtaccgg tgtgatggtc aatatgtatt ccgttccaag cagattgcct
    20581 gatgcatcaa accattgata aacattgcct cctgaaccta taagcgatac ttcttcacca
    20641 aggcatattt tcttatcttc gcctgcatct gccactacct ttaccttagt tcctgccagc
    20701 acaaactggt tagctccttg tattaaagat tgattctgga aactgatatc gcctgccttt
    20761 cctccgttta tctgcgttcc attgacggat ttggtctgcc agcccgattg ctcatagtca
    20821 tacaccggtc ttgagtaaat cttagaaccg gtagcctcaa tacccgattg tagaaatgat
    20881 aattcggtca atccgttgaa ggtcttgttg cttccccagt tttcaagtat ccagtaatgg
    20941 ttggatagtg aaggcactcc aggttggcca aaaggtttgt tgtagagcct ggtcatcacc
    21001 atttcaccat taggtaagct gccaccagtt ggaaaattca gagtgactct cgaatttgga
    21061 aaatctgtct ttccgttggc attaatcgtt attctgtctg aaacacctgt gccaatagca
    21121 gcagtagatg acaccagaag cctactgccg ttgagaccat gactttggct tattttgtca
    21181 tagacaatat ctgccttgct ttcattaaac taatagtaaa gcacaagact cgaatccgga
    21241 tttttatcta tcaggtgcat ctgttctcgt atctcggcct ggctaaatgc tttattataa
    21301 atcctgaatt cttccatttc gccattgaat gttccacctt gaccataaac agggcatccg
    21361 atttcaaagg gtgtttccga caggtcaaaa ttgctaaagt cgccgttata tacccacggt
    21421 ttgccgtctt tgtagagtgt tactttggtt ggttctactg tcatggctac atgtatccac
    21481 ttattgagtt cgaggtcgaa tgaacttgtc tgccagtaag gtacgttttt ccaggaaaaa
    21541 gtaagattgg tagtaggcac ataaccctgg aaagcaaatc cgaaaccaaa gcctacatta
    21601 ctgccccagt ttgatacaag ttgtgtgaac cattgttgtt tatcagcgag ttttatccag
    21661 cacatgacag tcatagactg tgcatttttc agttttctca ctgcagggat gctcacaatt
    21721 ttatgatctc cggtcatttt cagacttttg cctgcaatgg tatccacatt acattctgaa
    21781 ggcaatattt ttatcatttt ggcaatgaat cttgtattgc tgccactcga gttgctgatg
    21841 gttaatttta catcatattc acctgcggat gggtaaacaa ctttgggtgt acgggtatta
    21901 gctcctataa ctgttgtagc acctgtgaac tcccatttcc aagattgtgt gccgtcgtat
    21961 ttcatgacag aataatcatc gaagtaaaat gtgtctcgtg cacaatatgt gtataacttg
    22021 tctgcagttg gttgtgcaag caagtttgaa ggttcataca gatttgcttc ccagaaacct
    22081 atattcgaac ctgaacgaat ttttccgtcc ctataaaatg gcttaagtgc tcttgatgca
    22141 tgattgactg ggagaccagt attgtactca gcccagtcag acattgtttt gtttctttaa
    22201 aatattcggc cattatcggc agctacatat acaccaccgt tggtaccaag ctggtgtaca
    22261 atatcctgaa gattgaaatc atttagtttg tcggttgtcc agtttagcca ggtatttcca
    22321 ccgtttggag aataatatac ctttttacca ttgctgctgt atgcaagtgc cacccatagt
    22381 tcgttttcgt tgtcaccact tagcgttatg gtcattaccc gtctttggcc tgatgatacc
    22441 ggaaggtccg gcaaagtact ccagcttaca ccaccattga ttgatttgta aatctttccg
    22501 tcccagagtg tattcgagcg ttgagtagca tatataacat tagggttact ccttgatatc
    22561 cgtatatgtt ccattactgc atcctggtcg gtactggtaa acaatgtaga gtaacttaca
    22621 ccattgttgg tacttttctt aatacttggc cctttgccta tatatacttc attccagcac
    22681 acggggctcc aagtcatttc gctgaactcc atgtagtaat acgattcatt cggccattcc
    22741 gataacggta ggttgactgt ttttccatca aaattttcgg gtatctcata accaccgatg
    22801 tctgagaaaa aagtctgtcg gttcttcacc ggattgacat aaccggtagc tgcttctgca
    22861 ccgcccattc ttaaaaattt cccctcagga taagcttgat agtaggcagt atttccattg
    22921 tgataacgcc cacctaccaa cacatcttca ttccaccctg aatcaaaacc ccataagtca
    22981 gtagcgaata ttccgttaat gcgtggttcg ctgttggcag caaaaaaatc gtcagaccag
    23041 gttattccgc catcggtagc tatataagta tcgttacctt ggctccgcat tgcctgtata
    23101 tcggggtgtc tggagaatgg tccgtaataa ccacctacag aagtaaaatt ggcgcctccg
    23161 tcaacagatc tgaatgcggt ggttgtagct actattattt tattggcatc aagtggtgag
    23221 gctaagatat cgaggtcata atatccctga ccattgtcca tttcgaaggc cggtgtactt
    23281 cctgtagctg taagtgtcca ggcttcggct gtattattgc tgcacatcag gcgtggtcct
    23341 tggtctgtaa gtaatattgc atatacgcgt gaagggtcgg caggtgttac tgctatacga
    23401 ccttcaccat atttgagacc tgtaggccag ccaatggttt tggccgtaaa actcgatccg
    23461 ccattggtag atttccagca ttcgtaaaag tcttttgtag tatttttctt caacacataa
    23521 acgatatccg gattggtagg atggaactct atatctgtac attcctcagt aagcgtttgt
    23581 gtccagtttg ctccaccatc tgtggtgcgg aatactcatt tgttgctaca tagcagtata
    23641 ttatctggtt tggtagggtg aatgatgatg cggttacacc aaaggtcgtt gttggtataa
    23701 atgttggtcc agctaaggcc gccatcagtt gattttctta ctacattatt ggtaccaagc
    23761 aataaggtac tagcatcatt ggggtgtatg gccagtgctt tggaattcaa cagatatgag
    23821 ccgcctctca gttcccagtt tttgccttta tctgccgaac ggaaaaatga gcctgtttct
    23881 gtaacgatgt acaagatacc aggatctgac ggcgctattt cgagacaata aatatttgcc
    23941 tgccacggac aagcatccat atctgcatta tcttctgatt tccaatgtgt ggtaagtggt
    24001 cctacaggtg tccaaatgtc attttgttcg gaactcagtg tttttttacc ttcggcaaat
    24061 tttctcttat ggttaatctg gtctgtactc atcaactgac cattttcttg aataaatggt
    24121 gttatcatcc ttcgccagcg acgataatac tttgtccaat cggttttctg atagctattc
    24181 tttttataat attcctgata atccttatca aggacaaaaa cattgggctg agctttgaat
    24241 aattcttggg cccatgccgg tgcagggttt tcggaatgct aagaccagaa atattttgag
    24301 cagaaatatt gctaacaaac ataatcattg caaagaagca gacagataac cgtatcattt
    24361 cttgtatagt tttgtaataa aatttcatca aaaatagtaa ataaaatttt gaaacaagta
    24421 ttcctacaca aatcgagtgt caatattctt gtttacaaag cataaattac cataaaccat
    24481 tgaaaactga catacaaaag cacaataaaa atcgttatta tgcaaatgtt ttaaaaattt
    24541 agtaaatttg cgcccctatg attttacgta aagcaggttt attgttggtt ttggttttta
    24601 cccttctgtt tgcaggatgt agtaacgaat ttgaaaacat ccgcaagagc ggtaatactg
    24661 aattaattta taaaaaagga ctggaatatt acgacaaaga agattacctc aaggcacaga
    24721 ctttgtttga gcaggtactg agtagttttc gtggtaaaaa agaatctgaa acactttatt
    24781 ataaatatgc ttacacgcat ttttacctca gaaactacat acttgccagt tattatttca
    24841 aaactgccgc taatattttt tctacaggtg aaaataaaga agaatctgag tacatgtcgg
    24901 cttactgtaa ttatatgatt tcgccagact atgaacttga ccagactgaa acactgaaag
    24961 caattgatgg tttccaggcc tttatcaaca cctacccaaa cagtaaacgt gtagaagacg
    25021 ccaataaaaa tatagatgag cttcgacgaa aattagcaaa aaaatcactc gcagaagcta
    25081 ctctgtatta taacacaaaa caatatctgt cggccattac cagttataac aacttattga
    25141 acgattatcc ggatatcacc gaccgtgagt atatcaaata catggttata aagtcaacct
    25201 atgactgggc aatgaggagc ttttcgttta aacaaacaga aaggtttaac atggtcatcg
    25261 acaaatatga agaatttgtg aacaaatatc caaaaagtaa gtatttaaaa gacataaaga
    25321 cattttaccg aaattctaaa tccaaactaa aagagattaa tctatgacag atattaaaac
    25381 tcaggtacaa ggcctagatt ctaatgtaca agcaagagat gtaacatcta tagcagccaa
    25441 aacaggcaac atctatgaat cactcaacat cgtggccaag cgcgctaatc aactggctat
    25501 tgatatcaaa caagagcttc acagcaaact tgacgaattt gcggtttctt ctgataccat
    25561 tgaagaggtt caggaaaata aagagcaaat cgaaatatca aaattctacg agcgactgcc
    25621 aaatccggta atcatagcgc tcaatgaatt tatgaccgac cagattcatt accattacaa
    25681 aaatcagact gaggaatcgg ctgagtaaca tttattgtta aaaatgagcc acctcgccgg
    25741 taaaaaaatc atcctgggcg tatgtggaag cattgctgcc tataaaagtg cttatttggt
    25801 gagatggttc atcaaaaaag gtgctgacgt aaaggtaata atgactgttg cagctaagga
    25861 ttttattaca ccacttactc tttctacgct tagtactcat cctgtcatct cggatattgc
    25921 agacgaagaa agctggaaca accatgtaga actcggtttg tgggccgatg cccttgttat
    25981 cgccccagta acagccacca ctctttccaa actagcaaac ggtaattgcg acaatatgct
    26041 tactgccacc tacttgtctg ctcgatgccc ggtttgggtt gctcctgcca tggatcttga
    26101 catgtggaaa catccggcta ccaaaagaaa tatcaacttg ctagtgcaag atggtgtcac
    26161 agtcatacca cccgccatag gcgagcttgc aagtggattg acaggtgaag gacgtatgcc
    26221 cgaacctgat gaaattggca tgttggtaga taaacatttc tcaacctcgc aacattggag
    26281 tggtaaaaaa gtactgatca ctgcaggacc aacatttgaa acaatagacc cggttaggtt
    26341 tattggcaac cgttcatctg gtaaaatggg tattgcactg gctgaaaatc tgagcagaaa
    26401 aggtgccgaa gtaacattgg tacttggacc cactcatttg ttgccaaatt gttcaaacat
    26461 tagggtcgta catgtagaaa gcgcacaaga aatgttcgaa gcagcaacag ttgcttttga
    26521 aaactgcgac ctaggtattc ttgccgctgc agtagccgat ttccggccgg taacagtagc
    26581 taacagcaaa attaaaaaaa cggactccga tttaaatatt tcactcgcac aaacacagga
    26641 tattgcaaaa taccttggct cggtgaagaa aaaagaacaa ctattggtag gctttgctct
    26701 tgaaacagat aacgaacacg aaaatgcaag aaaaaaaata atatcaaaaa atttcgacct
    26761 tattgtccta aattcgttaa aagacaaagg agcgggcttt caacacgata ccaacaaaat
    26821 aacaattttg actaaagaca ataaacaact gcattttgag ttaaaagata agtccgaagt
    26881 ggcagaagat attgtggtag ccattgaaga ttattttaaa aaatgaatta tgtcatatag
    26941 aaataccttt cttgctttta ttttcatatt attaactact acccttcatt ctcaggaatt
    27001 gctttgcaaa gtagtggtca atactccaaa attacagacc gctgacccta aagtatttca
    27061 aaccttgcag actgccatcc gggattttat gaacaacaca gcatggacgg atgttagcta
    27121 cgataatttt gaaaaaatcg atgtaaatat tacactgaat attaccgagg aggtcaatgc
    27181 caatactttt cggggcgatt ttgccataca aatggtaaga ccagtctata actcagatta
    27241 taaaacgcct gttttttcgt atgtagataa ggattttgca tttatctatg aacaatacca
    27301 gccaattcag ttttcgagag gtgtatttaa tgataatcta tcctcgatgc ttgctttcta
    27361 tgcctattac ctgatagctt tagactatga tactttttct gcacttggtg gcgaaaatta
    27421 ttaccttata gcacaggata tcatcaatgc aatacctcag tctgtatctg gtgcattcaa
    27481 gggatggaaa ccatctgacg gaaacagaac aagatatttt cttcttgaaa attcgctcaa
    27541 tccggggatg aaggctgtca gacaaggatt ttatgaatac taccgcaaag gtctggacct
    27601 gatgagccaa aatccggaga caggcagaga aggcttgatg aaatgtcttg aatactgtga
    27661 tgctaccaac agatcgtacc ctaattcttc agctgtacag atattctgta ctacaaaagg
    27721 cgacgaaatc atcaacattt ttaaagctgc agatgctgca caacgagcaa aggttataca
    27781 atacatgacc agaatggatc ctgccaatac caataagtac gttcaattat catattgaca
    27841 cttttttaaa atttaattct catttccatt ttattaagct aacattccca tattggagtc
    27901 tgtgtagtaa ctttaccgca ataaataatt tgacaaaatg actttaagag acggtttcga
    27961 ttacgcacac accaatccgg cttggtttat cttctttttt gtgatgttac ctgtagtagc
    28021 gttacttgta ggttggatat caaaagaaga aggtatcaac agcccttggc gtgagattta
    28081 tggggcattg atatatttgg cttgcgtacc gggaatattt gccgtaacac ttgatatcta
    28141 tctgtttttg ttcgaaaggc aaagtatttt tgattcaaat cttgtcttac aggtgttgcc
    28201 catcataagt atggtggtta ctttacaaat catcaagcat aatgttagcc ttgaccttat
    28261 accgggattt aaaaagcttt cgggtctagt aatgattatc ttctttacca tttctatcat
    28321 gtggattatc gacagaaccc atatatatgc gatagctttc ttatcattcc aggcagtgtt
    28381 ggtaatattc gttgttttat tcctggtggt cagatatgga tggtatctga tggtaagtaa
    28441 ataatttttt cgtttctaaa taatccttta ttgacctgta ttatgactgt ttacttgatt
    28501 catggttttg gtatgaataa ttctatctgg agaaaaatta caaaaaatat tcaggcagaa
    28561 aacataatac tcactaacct tccaggctat gatggaactg atgaaaaata ttacgataat
    28621 attgaatcac ttgccagtgc ttacaaaacc ctaatcaaac gacccggaat aatcgtaggc
    28681 cacagtatgg gaggttatct cgcattgcag atttgtaaat tatttcctga aaaaattcgt
    28741 ggccttatgt tgttccattc gcatcctttt gcagatagtg gtgacaaaca agaaggtcgt
    28801 aaaaaacata tttctttttt agaaacgcac ggtgttgcca aattttacaa agaaataatg
    28861 cccaattttt tccacaacaa agccaataag gaaatagaac aacttattaa caatggaaac
    28921 aacatgcgtg ttagtcttct tgtaaataac cttcaaataa tgacaagccg tccggatatg
    28981 actgatttcc ttaccaaaac caatatacct gttggtttta ttattggaaa atacgataat
    29041 ctgattgatg gtgaagcttg gtttgcacag acggcttttc ctaaaaaggc tgcagtttat
    29101 gccttaaaaa agtcagcgca tttgggtatg ctggaagaac cacgtaaaag tactgaagca
    29161 atcaatcatt ttattgaaac atgtagctta ctttagtttg tcattgtttt ggtgtcggtc
    29221 gatatctctt tctgttttct ttttaaggtt gttggttaat gcttcatcga gatcgacacc
    29281 ggtttggttc gcaatacata ttaacacaaa caatacatct gccaactcat cggtaagcat
    29341 tgcttttgct tcatcatcag tggtaggatc tttaaaagat tgttctccat aaattctcga
    29401 tatcaatcgt gctacctcgc ctgtttcttc ggtcagaatg gccatattgg taagttcatt
    29461 gaaatacctg acgcctactt ttttaatcca attatcaact aatttttgtg cttctcttaa
    29521 ctgcatttat tccttttttt tcgtgtcaat aataatggtt acaggaccgt tatttaccag
    29581 gctaactttc atatctgccc cgaatattcc tgattttaca ggtatattta attgattttt
    29641 aataaattgt aaaaaataat tatacagtgg tattgcaata tcaggttttg cagaattaat
    29701 aaatgaaggt ctatttcctt ttttggtcga agcaaaaaga gtaaactggc tgatgactag
    29761 caactcacca ttgatatctg taatatcgag attcataagc ccggtattat ctgcgaaaac
    29821 tctcattttt actatttttg atgcgagcca ttctgcgtct tgttttgaat cttcattttc
    29881 tatacctatt agcacgagta atccttcgtc tattttgcct attatagtat tatcaacctc
    29941 tactgatgct ttgttcactc gttggataac cgctctcata taaaaatttt aatcaatcct
    30001 taggtgcatt tgaaaactat cgttaaaggc aagcagctca gcagcattga cattgtatga
    30061 gtcaagcata tttgaatggt ctgttaatag tatatcttca atttcgttaa tttggaggtt
    30121 agacaaccac tgctttctta gttcgctttc atgtattatt agtggcaatc tggtattcgg
    30181 aaaatattcg gattgttgag taataatact aaaactcaag tggttataac catctatatt
    30241 gcatgagtcc catacacctg ctaaagtgag taattctcca ttttttgaaa aatatctttt
    30301 cgctgtactt tgtgatgggc cgaaatgttc ttgcaaatca taaaaactgt caactacaac
    30361 gatgcatctt ttttgtctgg ccggaattct gaaagatgta cttgtcagca gagtttctgc
    30421 acgtgcgaga gcaagttttc cttcgttttt aattatttgt gccgtttgtg gtataatgcc
    30481 ccaggtaaca tagtcaagcc tgtcaggtgt actattcttt attatataag tatgatgagt
    30541 gggtgctata ttataagaag tacgcaagag tccttcagat tggattccta tatccttcca
    30601 tattttttca gaggtagtca caaatgaaag tctgctacac atattaccaa caatctatta
    30661 aaaaaaattt ataagtattt gaaacttaga agcaaattta aaaacttatt cacattaaaa
    30721 tgtttaaaac tcgatatctg tcaacaatag aagtaaaaac atgtagaaag aattacaata
    30781 aaataatttt taaagaagtt ttggatgaca gtcaaactgt taaacacatt caagctataa
    30841 acaccaacat aaaagaattg ccaacatata tatatcaaca aatttgacaa gattgttaag
    30901 aatactacta tgatgctttt atttgtttaa taaagtatat gttgataatt ctgaaaacaa
    30961 tgttagtatt tatatggaaa aataattaag tacaagttat taacatctta acaacactga
    31021 tgatgatgaa tttatattaa ataattaaaa ataaaattat cattatcagc ttaaattaaa
    31081 tgtttttgga aaagtagttt ttaaaaattt atatttgcag aaactaatgt agttcacatg
    31141 tcaggtaata gaataactgc agtacaaata tggttgatag ttgggttatt tatgatacta
    31201 atgcaggtat tcattggtgg tattactaga ttgaccggtt caggacttag tattacaaaa
    31261 tgggatattg tcagtggtac cattcctcct ttgaatgaaa atgagtggaa tgcacaattt
    31321 gaactttatc aacagacacc tcagtatcac aaaatcaata aaggtatgag tttgtcagag
    31381 tttaaattta ttttcttctg ggaatatttt catcgtcttt gggcgcgtac tatgggtttc
    31441 gtttttttat ttccattgat tttttttatt tttaaaagga agattagttc attgttagga
    31501 aagcgacttt tcattgtatt tttattggca gctttaaatg cttcaatggg ttggataatg
    31561 gtagctagcg ggcttattga acgtccatgg gttaatgctt ataaactatc gtttcatttg
    31621 atattagctt taattttatt tttatattta ttgtgggtag taattcagga gttcagagta
    31681 gagaaggaag attcaattga tgtaaccaac aggatagcat acaaatcctt tttttggata
    31741 ttatttgtac aaattttttt aggtggaatg atgtcaggta tgcgtgcgtc gatgatttac
    31801 cctacatggc caaagattgg agacagttgg attcctgcag tattgactga tttctcgcat
    31861 tggacataca ccaattttgg gaagtacgac gaacatcatt tcttgcctgc tttggttcat
    31921 ttccttcatc gcaatttggc ttatttaatt ttatgttttg gtttatattt atttttcaag
    31981 aatataggta gagtaaaatc agctttatta aagacaggat tataccttca gattacattt
    32041 atgtttttac aggtcatttt aggtgtactt acattgatac attcaaaggg aaggatacca
    32101 atttggtacg gtgtatttca ccagttagtt ggttttttgt tgataagtgc tattgtttat
    32161 attttaagtt tagtgcatga ttcccaaaaa acggtttagt tttagtatat atttggaaaa
    32221 ggtttataaa tattaataga agcaaacaat atggaatacg aactgataga ggaatttgga
    32281 ttcaaatata ttgagaccaa cggtggaaaa gaaactctgg ttttattgca tggattatta
    32341 ggggccttat caaattttga aggaataatg aaagaattcc atgataggta taatgttgtt
    32401 attccaatac taccaatcat ggaattacct atgttgaagt tatcagttgg tggcttggtt
    32461 gatcatgtgt atgattttat tcggttcaag aattttgata aagtgcatgt attgggcaat
    32521 agtttaggag ggcatatttc tattttattt gcattggcac atccgcagca cgtagcaact
    32581 attacactta ctggtagtag tgggttattt gaatctgcaa tgggtacatc atttcctaaa
    32641 aggggaagtt acgaatacat tgaaaaaaaa gcccaagaga cattttatga ccctgcggta
    32701 gctacgaaag aattagttga tgaaatattt tctacaatta gtgatcggct taaagctctg
    32761 agaattgtag taacatcaaa gtcagctgtt aggcaaaatt tagaacatcg gcttcatgag
    32821 attgaaaagc ctactttact tatttgggga catcaagata tcgtcactcc accatttgtt
    32881 ggagaaaaat ttcacagttt gataaagaac tcagagctgc attttataga taaaagcggg
    32941 catgcgccaa tgatggaaag accggaagag tttaatgcaa tactttcttc atttttagat
    33001 agaaatcgtc ttttgtaatt ttattaatgt ttcacgtgaa acattaataa gtaattgatt
    33061 tttgaataag tactaaagca ccattaacat tccattttga tttcttgttt acatttaatt
    33121 gaaaattcgt gtacctttgc gcccctaata aacaattaaa tgcagaatat aagaaatgtt
    33181 gcaattattg cccacgttga ccacggcaaa acaactctgg tcgataaaat gctattagca
    33241 ggcaaacttt ttaaagatca tgaaaaacct ggtgaattat tgctggataa caatgacctg
    33301 gagcgagaaa gaggaattac catccttgca aaaaatgtag ccgtacgcta taaagattac
    33361 agaataaata taatagatac acccggccac agcgacttcg gcggtgaagt agagcgtgta
    33421 ctcaatatgg ctgatggtgt tatattattg gtagatgcct ttgaaggacc gatgcctcag
    33481 acacgattcg tactccaaaa agccattggc ttgggactca aaccaattgt agttatcaat
    33541 aaagttgata aagaaaactg tacacccgac gaagtacaag aacaagtatt cgacctgatg
    33601 tgtgcattgg atgctaccga agatcaactt gacttcccta cattgtttgg ttcagctaag
    33661 aacaattggc tggcggatga ctggagaaac ccaaccacag atatcaccca gctgtttgag
    33721 gctatcgtaa aatacattcc agcacctatt gtatctgccg gcacaccaca attaatgatt
    33781 acaagtcttg actattcagc ctatataggt agaatagcta tcggcaaact tacaagagga
    33841 accttgactt ctggtcagaa tgtttctctg gtaaaaagag attcgagtat aaccaaaaca
    33901 aaaatcaaag aactttatta cttcgacgga tttgagaaaa taaaaaccaa cgaaatattg
    33961 gctggtgata tttgtgcgat tgtcggtctt gaaggttttg aaatcggtga tacagttgca
    34021 gattttgaaa atccggaagc acttgcaacc attgcggtag atgaaccaac tatgagtatg
    34081 ttgtttacta ttaatgattc acctttcttt ggtaaggaag gtaaatttgt tacatctagg
    34141 cacatcaaag aaagacttga aaaagagcta gagaaaaatc ttgctctccg tgttgaaaaa
    34201 actgattcgg ctgattcctt tatggtgtac ggtagaggtg tacttcacct ttctgttttg
    34261 atagaaacca tgcgtagaga aggatatgaa ctacagatag gacagcctca ggttattttt
    34321 aaacaaatag acggacaaaa atgtgagcca atcgaagagc taagcatcga tgtccctgaa
    34381 gaattttccg gaaaagctat cgaactcgca actcagagaa aagctgaaat gaaacaaatg
    34441 gaacctaaag gcgacaggat gttacttagc ttccacattc cttctcgtgg tattatcggg
    34501 ttaagatcaa atcttctcac cgctactgcc ggggaggcaa ttatggcaca tcgttatatt
    34561 gagtatcagc catataaagg agacatcaca acacgtatca atggttcttt gatttgtatg
    34621 gaaacaggcc aggctatacc atattcattg aataatctga aagacagagg tcgtttcttc
    34681 attgaatcta attcggatgt gtacgaaggc caggttattg gtgaaaataa tagatccggt
    34741 gacttggtcg tcaatattac caaaacaaag aaacttacca acatgagagc ctccggttct
    34801 gatgataaag taaaattacc tccaccactc attttttcac ttgaagaagc attagagtac
    34861 atacaggctg atgaatttgt agaagttact ccacattcaa taaggcttag aaaaatttac
    34921 ctgaaagaac acgaaagaaa gaaggcaggt aaagttgttg aaacagttta aataagtaaa
    34981 aggtgtcatg tgaaaatttg acaccttttt tgtttttcag atcaaagttt atcattattc
    35041 aatagtatga ccttttggta attcttgtct taatatctga tttgcacatg gaaaaatact
    35101 gagtgtgact ttaatacaca gaatgaagtt gcaaaatatt tggtaaaaaa caatatattt
    35161 ccaaaataat attttaattc tttgacgaac gcttgaaagt catctaaaat attgcattta
    35221 acgagcgaaa aaattaacat tttgttaaga gtaattttaa ctttatcaat ttagtgttaa
    35281 aatttttgct cagtatgaag aaaatataca tttgcctaat ccttcttttg tttgcatcag
    35341 aagctttttc ccaaacatat ccaccatgta ctaatggctc acaattagta tgtaaatgta
    35401 atacagcccc attattatgc accatcgatg aactggatgg gtatgttctg acgatgaata
    35461 actaccaaca cccgggtgac gcacctacgc ctctatgtcc tggttcaaat acagtcccaa
    35521 acaacccgac atggtttgca ttcaaagcat ggtgtactga cctgacactg aactgtacac
    35581 ttactaattg tactggtaaa ggaggttcaa atggtgtaca aatagccatt tataaccaat
    35641 gtggctctaa tgatgccgtt gcctgtaatg tcaatgattg tggaaataca aatgataaaa
    35701 cattatccat gtctggcctg caaattggta aaacctacta ttttatgata gatggatgcg
    35761 caggctctta ttgcactgtt accatcgaca tcattggtac ctgtggttct gaaaccattg
    35821 aggactggaa aggacccatc atgggagaaa cagaaatatg cgcaccaaaa actgaaacct
    35881 atactgtaga taaattggat ggcgctacac attatatttg gtatcttgat ggtgcggtag
    35941 taaaaaatag tacatcaaat tcatttacaa aaaactggac aactgccggt acttacacct
    36001 tgtgcgtaga tgcgtataat gatccttgca ttcctgtaac caatcctcct gcacaaaatt
    36061 gtataacaat tgtagtcaac tcggccaatg ccggtaccat cacagctgca ccgacaccat
    36121 tgtgcccaga tgcacctgtt acgtacaatg tcacaggata tgagaccaac cccaataata
    36181 ctgaagttgt ttttgtaaca aatgcagcag gtgaaattct ggaaattttt cctggtgtta
    36241 ctggtacatg gacatcttca gaatgtggaa cattcacctt ttattcatat aattatgtta
    36301 caggcagtaa tgcctatgta cctgtagtag gaggtaatgt aagttcaata gactgtactg
    36361 ctaactgttg cgatctcaaa agtaaatctg taacattcca ggatacgcaa aaaccagtac
    36421 ttcaaaataa acctccaaat cagactttga gctgctatga cttactacag cctatgcagg
    36481 cattgaatta tacagataat tgtatcccta acggttcggt tccaggtgaa gagactggta
    36541 gtgccgactt gtgttctggt ggtaccatta cccgcacctg gacaattgaa gataattgtg
    36601 gcaataaaga ttcacacact cagaccataa ccatcaatgc tgtaccagca ccaacatgga
    36661 aatcaacttt gcctcaaaat cagacactta catgcagcca aataccaact actcacccaa
    36721 accttgacta cgaaaataca ggaatgggtg cctgtctggt gagcggaacc gtagcgcctg
    36781 ttgttactgg tagtgccgat ttgtgcggtg gtaccatcac tcgtacctgg acatttacgg
    36841 ataactgcaa ccacacgatt aatcatgaac aggttttgac agtgaccccg gtacctgagg
    36901 cgagctttca gaatccaccg gcaaatatga ctattacatg tgaccaaata ccaacgtctc
    36961 atccctcact tacatacacc aacggtctga gtggtgcctg tggcattagt ggtacatcaa
    37021 tacctactgt cacaggtagt gctgatctgt gtggtggtac aataactcgc acatggacat
    37081 tcacagatgc ttgcggacgt acaaaaacac atgagcaggt tttgacagta actcctgtac
    37141 ctcaggcgag ttttcagaat ccaccggcaa atcttaatgt tgattgttcg caaataccta
    37201 ctactcatcc ggatctgagt tattcaaacg ggcaaacagg agcctgtggc atatcaggta
    37261 gtgtgtctcc tgtagtcaca ggtagtgccg atctttgtgg aggtactatt acccgtacat
    37321 ggacattcac agatgcttgt aatagaacca ttacacacgt tcaaaccatt actgctactc
    37381 cggtaccagc agctgccttc cagaatgcac catcgagcca gacagtagat tgtgcccaga
    37441 taccaaccgg agcatcagac ttaagctata ctaatggtct gagtggcgcc tgtggtatca
    37501 acggaagtgt accagcagtt caaagcggta atgcagatct gtgcggtgga accattacct
    37561 atacctggaa ttttacagac gcttgcaacc gtaccattac acatgttcaa acatttacag
    37621 ctaatcctgt acctgtagcc acatttcaga atgcaccatc gagccagaca gtagattgtg
    37681 cccagatacc aaccggagca gctgatttga catataccaa cggtctgagt ggcgcttgtg
    37741 gtatcaatgg aagcgtaccg gcagttcaaa gtggcaatgc agatctgtgc ggtggaacca
    37801 ttacctatac ctggaatttt acagatgcct gcaaccgtag cattagctgg gttcagactt
    37861 ataatgtcaa ccctgtaccg gtagcttctt tccagaatgc accttcaagt cagacagtag
    37921 aatgcgaaca aataccgacg agtgctcctg cactaacata taccaacggt ctaaatggtg
    37981 catgtggtat caatggttcg gtttcaccgg ttgaatcagg ttttgctaac ctttgcggtg
    38041 gcacaaagac ctatacctgg aatttcacag atgcttgtaa tcgcactatt aactgggtac
    38101 agaattatac agtcaatgca gtaccggtag ctactttcca gaatccgccc ggagataaga
    38161 ctgtcaattg tagtgaaata ccggtatcag tacctgactt gaactatacc aataacagaa
    38221 caggactatg tgccataaca ggaacagtac cagccgtcaa atcaggtaat tctgatctgt
    38281 gcggaggtac aactacctat acctggacat atacggacgc ctgcttcagg actataagtc
    38341 atgcgcagaa ttatactgcc acaccagttc cggttgctgt tttccagaat ccaccgggag
    38401 atcaggtagt ggattgtgaa aatataccag cttcggctcc aagtttgtca ttcagcaatg
    38461 gactttcggg cgcctgtggg ataaatggct ctgtaccagc cactttgatt ggtagcccga
    38521 gtgtttgtgg agggacgttt ggctacaact ggacatatac ggacgcctgc ttcaggacca
    38581 tatcgcatac acagaat
//
LOCUS       DAOQQY010000002        38392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_300_length_38392_cov_7.262697, whole genome shotgun sequence.
ACCESSION   DAOQQY010000002 DAOQQY010000000
VERSION     DAOQQY010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 38392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38392
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_300_length_38392_cov_7.262697"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2252)
                     /locus_tag="PLE23_00165"
     CDS             complement(<1..2252)
                     /locus_tag="PLE23_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUB domain-containing protein"
                     /protein_id="HMX81705.1"
                     /translation="MTGIRLVFTYFLAFISITVVVAQPSFNMSNATTNECEGILMDSD
                     NGMLPDTYDNNENYTFKICVPGGEKIIFTFAKFCTEEKLDYLRFFDGPDTLSPMIGGP
                     FSGTVLPPAITATSGCLTINFISDANIQCLGWLGSWKVEIDDPLPPQMMVTGTVPCES
                     SSFKLKLSQPVSCDSIYPGAFLLYGPQSPDIVSATPVSCVNGKTDEILLSFSPEIKVN
                     GNYTVNFTTTFLDVCNKPHILTATANFNVSGCPLYVELVPEGNKTKVCPGECLKLYAN
                     VSGGDPSTYNIKWSDPTFPNTSAVDVCVPPLTYSVTVTDGIGSKPAVATFTFELHTPP
                     VINWPLDLTICQSEPSINLGATPGGGTWECNGIDDKKKTPGLYEPWRIWQGSDQIIYT
                     DKNGCKDTVNITVKPIWINHDDASCPGAAPFIVSGGSPVGGTWSGQNILPNGTFDPSA
                     LGTYFITYTAPNGCSQSKKVNVANLVMPADITICSSLSDFSIPVSPWGGYWDASPGLN
                     ANWGWFSPANALPGENKLHYHINGCQDSMSIYIVPVDAKYDFVACTNNGPFIVPGSWG
                     PVGGVWSGPGIIDTLSGLFDPSLLKDGQNITLTYTVNGCSDNRIAYIRNTVINNKSVI
                     SFCPYDDVYKILNDDFQAKPNGGSWSGPGVYYVYIKDKDPKNGYYFDPKQAVTGLNTI
                     VYSNNGCTDTMFFNINPLPVLDSISLCAEETPIKLNASVPVSTWVGNGIINAFGGIFD
                     PKTAGTGQHI"
     gene            complement(2259..2702)
                     /locus_tag="PLE23_00170"
     CDS             complement(2259..2702)
                     /locus_tag="PLE23_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81706.1"
                     /translation="MIRIVLVLFILSMMLSCQKDDNLLPLSTTPSIRLLKISSDTIKE
                     FQDPLELLIEYEDGDGDLGNIDTDINSLFIKDNRLDKADEYYVAPLAPIGSQISIRGT
                     LNITLNGAFVLGNSASEKTYFELWMIDRAGRKSNVIRTKEITINK"
     gene            complement(2699..3640)
                     /locus_tag="PLE23_00175"
     CDS             complement(2699..3640)
                     /locus_tag="PLE23_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81707.1"
                     /translation="MTRIFSLIVLFGVMWAGCEKEAPNPYKDFQPNNQDTVALYKPDP
                     KSFAGLHAYIFKPTCANSGCHDGTFEPDFRTISSTYNTLVYHPIIKNDPQNSFTYRVK
                     PGSSAESVLYKRLTADIDGQSGIMPLVVDPGSDWNSKKDEYIANIKAWIDGGAKDIFG
                     NLPSLTNKVPQMQGVLGKTGTWLDRADSGQGALRVKETETSLDLYFAFSDDKTPSDQL
                     TNARIKFSKSQDDFANATSFPLEVLSTPVDFTGYYGNNVHYYHKYSLNPQNYAKTGET
                     IFFRVYVTDSDGIETEIPTNDGVYYIKNYFSFKIIIG"
     gene            complement(3651..4994)
                     /locus_tag="PLE23_00180"
     CDS             complement(3651..4994)
                     /locus_tag="PLE23_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81708.1"
                     /translation="MKRRSFIKKMALATGGVLAAPYILPSGRLFATTNPPLAEHVVLV
                     LFAGGVRQQESVQQRYLSDSQKLNIEGNIMYNMLEGDAPTLKVAYGTTLPGGQKGGQP
                     ISKVLPTSLQKQGTLFKELRFSKGSTGHYNGLATAVSGNYSVTQGLKQRPLHPTIFEY
                     ARRFGGLKATDTWFVGNGISGSIPLLNHSNYEGFGERYGANFIAPIVTFGAEKYIKGF
                     KIYHPDDELDPIHKMQTFLNQSFRLQGSHLETLGNTEEERESIKDFIRKTFDRLDKQQ
                     IAFPPVTDNGDNITIGFATEVLKYFKPKLTVINLNDVDVCHGNFTTYLGALHRADHGV
                     GHLWDVIQTQIPEMSGKTAMLIIPECGRNLDHNPITDENDWFAYDHSDQNSRRIFSMM
                     VGPGIPSNLTIGSEGNPVGDSSDIIPTIAELLGFKNDVLSQGLIDPVAKSFFDQI"
     gene            complement(5017..5499)
                     /locus_tag="PLE23_00185"
     CDS             complement(5017..5499)
                     /locus_tag="PLE23_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81709.1"
                     /translation="MKTFNIFFIIVLFVLVGCKDDKYIYEVNKVDILPNNADKNKEKT
                     NEQYLNIVYANLYQKALSPDKLVDLSNVIQSIGDKQVAHEIIISKMMNDPDIKLPPLS
                     EMKADPEKFIINTYKRFFVREPTQAEKAFFLNFIKSHPNITPEHIYFAFATSAEYDYY
                     "
     gene            complement(5510..6421)
                     /locus_tag="PLE23_00190"
     CDS             complement(5510..6421)
                     /locus_tag="PLE23_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81710.1"
                     /translation="MNKLFITLFTLVVVVLTGCNKDPEIIGGNNPPDYTKISTIKMQN
                     YVNRMFIDLVGREPLDVEMDENVNLLRSNNLSVNARRQIIQKLQNSTDSVFGDGSYRK
                     AYSQYMYNLAKVRTIEGASDEDINMRIGNIVFGMKEDSLLGDWESYNKGLLEKNNLQS
                     ILDTKVQFFDGQIPYDVFFSRMVNNSIYDFIHMNTFNFVNSTFEDLLWRFPTSTEFLV
                     GFDIVEYNKPNLFFNKLCTNKKEYVDAICDTNEFFEGMIIWCYRTLLSREPKSQETSE
                     ILGEYILNRDIRKIQEKIMITDEYANF"
     gene            complement(6494..6943)
                     /locus_tag="PLE23_00195"
     CDS             complement(6494..6943)
                     /locus_tag="PLE23_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HMX81711.1"
                     /translation="MSLEAKITEDLKEAMRNKDQAALRAIRAVKSAILLFKTSGTQEE
                     LNEENEIKLLQKLVKQRKDSLEIYRQQNRPELASVEEEEIAVIEKYLPKQLSAEELSA
                     FISKLIADNGVSEMKEMGKVIGLASKALAGQAEGKAISEEVKKQLSK"
     gene            complement(6927..7697)
                     /gene="rsmA"
                     /locus_tag="PLE23_00200"
     CDS             complement(6927..7697)
                     /gene="rsmA"
                     /locus_tag="PLE23_00200"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMX81712.1"
                     /translation="MKAKKSFGQHFLINKMAVDRIVEKFGAINATGKALEVGPGMGVL
                     TEKLLSTYPSLTAVEADHDMVAHLHNRFPLLNVVEADFLKTDLKQLMNAQEFGLVGNF
                     PYNISSQIVFKMLDYKEYVPVMVGMFQKEMAERIVAGPGSKTYGVISILTQAYYQGQL
                     LFHLSPSDFNPPPKVDSSVIILTRKEDTGPQYNNRLFTELVKTTFNQRRKMIRNTLKQ
                     YLPEHILNEPFYQKRPETLSLEDFINLTINIENYEPGS"
     gene            7795..8862
                     /gene="pdxA"
                     /locus_tag="PLE23_00205"
     CDS             7795..8862
                     /gene="pdxA"
                     /locus_tag="PLE23_00205"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HMX81713.1"
                     /translation="MDTIKIGITVGDINGIGLEVILKTLSNNLVYKNFAPIIYGSPKI
                     VSYHKNIINLPDLHINQVKTPDQAQQGVINLITCWQESVNISLGQASAEAGKYALISL
                     TEATKDLKAGDIHGIVTAPVDKQVIHNEQFPFIGHTEYFAKEFNSPTVMLMTSPNVSV
                     GMVTDHIPLKDVSRSLSKDLIIEKIKILHKTLRRDFGYEKGKIAVLGLNPHAGDKGLI
                     GDEELSIIRPAIVECKNQGIFVFGPYSADGFFGSAQFKKFEAVLALYHDQGLIPFKTI
                     AFSEGVNFTAGLPVVRTSPDHGVGYDIAGQNVADETSFRHALQMAFDVVRNRRSYDEA
                     HNNPIAKRAKNFEDEVLKDEN"
     gene            complement(8859..9560)
                     /locus_tag="PLE23_00210"
     CDS             complement(8859..9560)
                     /locus_tag="PLE23_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HMX81714.1"
                     /translation="MKRILTFMLIVSAFTNLTLAQSYKEKVNNYIRKYHHIALENQTR
                     YNIPACITLAQGILESNAGYGMLADSANNHFGIKCKDQWLGKVYYKEDDDLDTLGNLI
                     LSCFRKYDTPEESFADHSLFLNTNPRYARLFSYDKKDYKAWATGLKDCGYATAPHYAK
                     TLIDLIERFNLNVFDEMDTSTENSYELYLTFNFINNQKATSSDNTELTSVKLPDYYQG
                     TYGLQNNTAAIAPPN"
     gene            9689..10300
                     /locus_tag="PLE23_00215"
     CDS             9689..10300
                     /locus_tag="PLE23_00215"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HMX81715.1"
                     /translation="MKKSYLVLVILFLVLLIDQASKIWVKTHMEYGEQFAIFGLSWAN
                     IHFVENEGMAFGMKLGGDYGKLALSLFRIVAVGFLAYLIINMIKERKSDFVLVCFTLI
                     LAGALGNIIDSAFYGMIFTETPYHSGGVATMFPPGGGYAPFLHGKVVDMLYFPLTEGE
                     FPSWVPFVGGDHFLFFRPVFNIADTAITTGVLLLLLFRRQIFH"
     gene            complement(10439..10771)
                     /locus_tag="PLE23_00220"
     CDS             complement(10439..10771)
                     /locus_tag="PLE23_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMX81716.1"
                     /translation="MKNTKVNIDNEKLELSSEILRALTHPLRLKIVEFIDSNKRINVN
                     KIYNSLKLEQSITSQHLKILRDAGILETKRTGKYIFYSLNYPRIENSVIAVNNFLAKQ
                     VKQTLIEE"
     gene            complement(10999..11337)
                     /locus_tag="PLE23_00225"
     CDS             complement(10999..11337)
                     /locus_tag="PLE23_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMX81717.1"
                     /translation="MRKQKNELILLKKGKNDIELDYAELRKAVLVLRAVNHKLRQNII
                     DLLEESDSLTVTDIYIKLRLEQSVASQHLAILRRAGVVITDRNGKFIYYSIDKERLSQ
                     ISRLVEELAA"
     gene            complement(11492..12169)
                     /gene="tsaB"
                     /locus_tag="PLE23_00230"
     CDS             complement(11492..12169)
                     /gene="tsaB"
                     /locus_tag="PLE23_00230"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMX81718.1"
                     /translation="MSKILNIETSTSQSSVAISVDSQVIAFKTLSPELNISGEITLLI
                     QSAANEAQIPLHSIDAVAINLGPGSYTSLRVGLSAAKAICYALNVPLIGTDTFEILYK
                     SVEDESAYDTIMCMVDARRMDVYARIYDAITNHFEKDEFVTMDEAFFSLYAGKQILCT
                     GDGAKKLSGTSYLPESSKISEITQNAAMMATISHEKYNNGTLLDIAYSVPYYFKSPNI
                     TTPKNIF"
     gene            complement(12169..12822)
                     /locus_tag="PLE23_00235"
     CDS             complement(12169..12822)
                     /locus_tag="PLE23_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81719.1"
                     /translation="MIQDPDKLYSSYINLIDRKDEEKILYFEKFHTLFESLEPYRYLH
                     CYIIYIESLLELNEYDEIDQKLDTAILTSLDIRQKERFETIYSILLFHKAQVKFLKGN
                     TDEAKRIMNELLKIKEPDEATINLLFHITATPTPRYIERLHNASVILMLITALLLIVE
                     LFQYKSFNSPDQFYLKIGIALSFFGGLFSAFCGDLFHYLKCKLLVKRLVGRHNTSKP"
     gene            complement(12830..13591)
                     /locus_tag="PLE23_00240"
     CDS             complement(12830..13591)
                     /locus_tag="PLE23_00240"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HMX81720.1"
                     /translation="MIIRLTHNGIHYTADLSTGTDISLPLLPDQPGVNCFYAPVFDAQ
                     PVVMGNFVGSTKQGGAVNFKNVKINPHGNGTHTECVGHISVEDVSINDTLRQFHFIAE
                     LISVFPQKSENADRVITEDILRAVFTNETKCEALVIRTLPNDDVKKTFHYSGTNPPYL
                     SADAISYLVNSCNIRHLLVDLPSVDREEDGGKLAGHKSFWKYPETLDLNKTITELVYI
                     DNTTKDGLYLLNLQIASFGIDASPGKPVLYKLYKE"
     gene            complement(13599..14480)
                     /locus_tag="PLE23_00245"
     CDS             complement(13599..14480)
                     /locus_tag="PLE23_00245"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HMX81721.1"
                     /translation="MKIAVYSKIFKQQDKACIQELFDVLHEEKLEVTVHNLYFNQLKN
                     HIDFQSNPDLYEKYEDLVIQEPDILLSVGGDGTMLGATTLVRDSDIPILGLNLGRLGF
                     LASLESKYLRQSVKQLKRGMYSLENRTLLHLESSVALFNGLTFALNDCTIIKRDTSAM
                     IRIKTYINGSFLNSYWADGLIISTPTGSTGYSLSCGGPIIFPDSGNFVITPVAPHNLN
                     VRPIVISDNSVISFEVEGRADTFLLTLDSRFESITAQHQFAVRKCDFCIKTVTLNDNG
                     FMQTIREKLDWGKDARN"
     gene            complement(14486..15850)
                     /locus_tag="PLE23_00250"
     CDS             complement(14486..15850)
                     /locus_tag="PLE23_00250"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMX81722.1"
                     /translation="MIFLPATSVYAQSVVIDSIVITGLNRTNKEIVLRDINIKPGDTL
                     VEKKIEAVLVDNRLFLMNTGIYSQVKITYENAKTKPHAINVLIHIDESWHIYPVPYFE
                     MVDRNFNVWARNYHFDLARINAGIKLYISNLTGNRDHLDGTFHLGFHDKAEASYWLPY
                     MNRKQNLGIQFTGLWANSKEVAYATTPENEQLRWFLDTTIIFRKKFSATLSYQPRLFT
                     KQKLKIEWQYYRINKSVLDKLQPDFFASPDGKMSYPVISYNITKDTRNIQPHPTKGYV
                     FNFLIEKQGINNIRDALITLIDFRYFQRLSAKWTATQQLRHQNNWINKATDYFHNRAL
                     GDELNYIRGYEYYIIDGQQYALSKTSLQYMLFDNYWNLGKFMPIKSLRKPQVQLFLAF
                     NFDMGYIPSAKNVPKTNFNDKLLKGGGIGLEIVAFYDKMLQIEYSMNEKYEKDIFLHL
                     NLSF"
     gene            complement(15919..16578)
                     /locus_tag="PLE23_00255"
     CDS             complement(15919..16578)
                     /locus_tag="PLE23_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMX81723.1"
                     /translation="MIAKDIISKNVTPIKIGDAAQKALQLMSEQMVKHLPVLEKGKLV
                     GVLSEETLLNCDAGEKIATLPMNFAGKYVYCKDHIFEILKHMAEHNMSVMPVVKDDMM
                     YFGIVTQDGIIKNFAQAESITEPGSIIVLETTKRNYSLAEISRIVENEGFSVIGLLIT
                     NSDNVELIDVAIKINTTNISAILAAFERYQYTIKVTYEENDYTDRIKDRFDSLMSYLN
                     V"
     gene            complement(16709..17452)
                     /locus_tag="PLE23_00260"
     CDS             complement(16709..17452)
                     /locus_tag="PLE23_00260"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NINE protein"
                     /protein_id="HMX81724.1"
                     /translation="MKNKYIAALLSLFLGIFGLHKFYLGDIKGGIFRILLFLALSMFV
                     EDWVFVLWLYSLMESVLFLTMSTEKFNQKYNEPNLHESRPWGRQPVAGTPFIKDNPYI
                     KTGNEKYKDYDYTGAIEEYTKGLALSPNDKVLHFNMACAYSLTEDADKSMYHLSKAIE
                     NGYNDFDTLRTKDDLAFLRVQPEFQELVNNNFRYTRPQERTEPATRPKQEVQEMPDLL
                     EQIKLLNQRRERGELTDDEYFIERKKILG"
     gene            complement(17466..18710)
                     /locus_tag="PLE23_00265"
     CDS             complement(17466..18710)
                     /locus_tag="PLE23_00265"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMX81725.1"
                     /translation="MSPLESKVKEKNEISDLEHKLSLKQLQLNGLMSITQAINDNVKE
                     QGLFQMYSDFINWQLGVKKMALFIQKDKEWVCATSIGAGDTVIDESLTHELHKYERLN
                     HLDNETHPGLMQFDVIIPVQHKNSPIAYVLIGNIENQDDSYGKVLFITTITNIIAVAI
                     ENKRLFKKQIQQERLNREMELASDVQHMLIPSKLPGNNYFNLSSYYLPHIGVGGDYYD
                     CISLSEESLIICIADISGKGLAAAMLMSNFQATLHVLLSKDTELLSLVKELNKSVYNI
                     AKGDRYITFFIAKIEATKKTIEYINCGHVPPIANINGMILELDKGSTILGGFEELPFI
                     EKGELSYKEVCTVLCYTDGITDILNNEGEYFSDALVHDLFRHSNNLTPDGLVEKIKTK
                     IHEFKQEQEYTDDMTVLVCKSY"
     gene            complement(18756..19850)
                     /locus_tag="PLE23_00270"
     CDS             complement(18756..19850)
                     /locus_tag="PLE23_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX81726.1"
                     /translation="MHNIFWQRISYNKSAFLSVTLLLLYFVVSLGCYILAVDNSADVN
                     TQIPEIALHPPFVSMTFISLQNKDTQTENRLKKAFFGSNIHTELIPVVSWHEAKDSII
                     LKKYTGKNQFTGEPLPTETITLSKSKELLNKEYKIIRKTFPFGTDAFGRCVYSRILVG
                     SRISLTVGLSAVLISLTIGILIGLAGGYYGGKTDTVLMLFVNTIWSIPTLLLVFAIVL
                     ALGRGVHILYLAIGLTLWVDVARIVRGQVMSAKQNVYIDAARSLGYDNKRIMFRHILP
                     NIIGPILVIAAGNFATAIILEAGLSYLGFGVQPPTPSWGNMLNENYGYALSGYYFLAV
                     IPALTIVLLVLSINILGNSLRDYFDVKIDK"
     gene            20101..20466
                     /locus_tag="PLE23_00275"
     CDS             20101..20466
                     /locus_tag="PLE23_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81727.1"
                     /translation="MNLHISDIRTIAEVKADFNKLFPYLRLEFNVNASERNGRTTSKN
                     RISDDVKIGSIRDQHVEANFEFNENSTVEEFENNFFEKFGLRVQVYRRSGNVWLETNM
                     TDKWTLQRQNEHGREITEI"
     gene            complement(20488..21546)
                     /locus_tag="PLE23_00280"
     CDS             complement(20488..21546)
                     /locus_tag="PLE23_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMX81728.1"
                     /translation="MKKTILIIDDHLEIRENTAEILELSGFNVLKAENGRVGVDVALS
                     SKPDLIICDIMMPGLDGYGVLHMLRNNHTTEHTPFIFLTAKTERSDFRRGMEMGADDY
                     ITKPFDDIELLHALETRLKKQEIIEKKYNRENNGFSNLVTDLQNTGALTLDISKYESE
                     HYSRKQLLYSEGKRPRYLFFLKSGKLKTYRVNEDGKEYITSFYSKGDYIGYLPLFENV
                     SYTDSAELLEDSEVVLIPKDDFLNMVFSDLRLSTKFIKLIAGDLADKEERMLRLAYDS
                     LRKRIAKALLEIQEKFDKENLTMTASITREDFARYVGTATESLIRTLSDFKSEKLIDI
                     KEGKIQILNKEKLNHLLF"
     gene            21669..24083
                     /locus_tag="PLE23_00285"
     CDS             21669..24083
                     /locus_tag="PLE23_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HMX81729.1"
                     /translation="MVVMDEKTSVKLHCEHCGLECENDLIKLEEHSFCCEGCKLVYTL
                     INETGLCNYYNLNEHPGINQRTSVRENKFSFLDDDSVESQIIGFRNNDFTQVVFYLPA
                     IHCSSCLYLLENIHRFNKGIINLEIHFARKEAKIKYKHKEVGLREVAELLTRLGYEPH
                     ISLRDVAGAKPKTSKSLIYRLGIAGFCFGNIMMMSFPEYLGLHGSEKSMTGLFRTLNL
                     LLAIPTFFYSATPFFTSAWSGLKNRFLNIDAPVSVAIIVTFGRSVYEVMAGVGGGFFD
                     SMSGIIFFMLLGRVLQDKTYQHLEFDRDYTSYFPIAVTTIQKGEEVPVQVSALKKGDL
                     VRIYNEEIIPVDGILSNGKAYIDYSFVTGESAVNRIETGEIIYAGGKQMGAAIDLLIL
                     KDVSQSYLTQLWKQTKVKSRTDQDQKSFVHYLSKYFSYLVFSISALAGIYWWIHNPAN
                     IFPAMTAVLIVACPCALLLSNSFSNGFIIRLLGYNKFFLKDALSIEFIAGIKHIVFDK
                     TGTLTDTSHYKVHYEGSELTQPEKLRIAATARQSRHPLSKAVSDYLGIRQVPTIISFE
                     EFPGQGTKSIFEGYEIKLGSEEFTGQMQNSDNRSRVCVVVNGEYKGKFYIENQYREHA
                     EEVIAGLKKKYQLTVLSGDNDKDAASLKKVFGENSTIIFNQKPEDKLIYIKNGQKQKQ
                     TVMMVGDGLNDAGALMQSDVGIAISDTRNNFTPASDVILGAESFSKLPALLRLCADGK
                     KIIIGSFILSILYNIVGVYFSVTAQLSPLVAAILMPASTFSIIIFTYTTSLVMARYRR
                     LSTGAA"
     gene            24170..24331
                     /gene="ccoS"
                     /locus_tag="PLE23_00290"
     CDS             24170..24331
                     /gene="ccoS"
                     /locus_tag="PLE23_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HMX81730.1"
                     /translation="MGVIFILLFVSLAIALLFLWAFLWSTKTGQFDDDISPGVRILFD
                     EEKSKTNNN"
     gene            24343..26469
                     /gene="ccoN"
                     /locus_tag="PLE23_00295"
     CDS             24343..26469
                     /gene="ccoN"
                     /locus_tag="PLE23_00295"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HMX81731.1"
                     /translation="MELEKFYYDNRPVKWFAYATIFWGIIGMLAGLWAAIAIYYPALN
                     LGWAPTTFGRMRPVHTNAVIFAFVGNGIFMGAYYSLQRLLKARMYSDTLSKIHFWGWQ
                     SIILAGALTLWAGYTTSKEYAELEWPLDIAIAVIWVLFGINMIGTILKRRERHLYVAI
                     WFYLATWVTVAMLHIINSIEIPISFLKSYSYYAGVQDALVQWWYGHNAVAFFLTTPYL
                     GLMYYFLPKAANRPVYSYRLSIIHFWALIFIYIWAGPHHLLYNALPDWAQSLGTVFSI
                     MLIAPSWGGMLNGLFTLRGVWDKVREEPVLKFMVVAITCYGMATLEGPLLSLKNVNAI
                     SHFTDWTIAHVHVGALGWNGFLTFGILYWLIPRMWNTPLYSKKLANNHFWIGTIGIVL
                     YAVPMYWAGFTQSLMWKAFTPEGQLKFQFLETVTHIMPLYITRSIGGFLFLTGTCIMA
                     YNLYKTIKAGKFAEQEEAEAAPLDKNWTAPSNDYWHRVIERKPVRMLVFSFIVVAIGG
                     IIEFVPTFLVKSNVPTIASVKPYTPLELHGRDIYVREGCYTCHSQQVRPFRDEVARYG
                     EYSKAGEFVYDHPFQWGSKRTGPDLAREGGKYPHSWHYNHMLDPQSMSPGSIMPAYPW
                     LFKDKIDTQKTKSMIWAMQKLGVPYEKGYGKVANNDLKKQSEEIVKSLKSDKIEAKPN
                     TEIIALIAYLQRLGKDIKAEGNSANK"
     gene            26489..26665
                     /locus_tag="PLE23_00300"
     CDS             26489..26665
                     /locus_tag="PLE23_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81732.1"
                     /translation="MKFVHYLERIVGVDIYPLLSLVIFGSFFLVVAVWLFSTKKSDFD
                     EVSRIPLNENEPEH"
     gene            26695..27846
                     /locus_tag="PLE23_00305"
     CDS             26695..27846
                     /locus_tag="PLE23_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HMX81733.1"
                     /translation="MENLILKQFINSSFLNGGFLLQVNPTATEQSPWMSVLTNPIAII
                     MILIMFALLFVIRALTKVLLEVAGNKMKRNLQDNKEGILKAIALLIVMITASSTGFAQ
                     DAAAAAVTTAPPKIIGGMAQSVFYVITAVIIIEAIIIYVLLMSIRKMVTKDNLDQYNA
                     GPESQGVIAKWWDQLNGYKAAEQADIDLGHDYDGIRELDNRLPPWWLYGFYATILFAG
                     YYLWTHHVSHSAPDSIQEYKTEMAVAEKQVAEYLKTKGDDFNENTVKMLGAEDIAEGK
                     KMFETTCAACHLATGAGSVGPNLTDAYWLNGGDIKSVFKTIKYGIRAMPPWQNSFSGK
                     QMAQLASFVESLKNTNVAGGKAPEGEEFKEGAGEAQPPADSTAAAAAQK"
     gene            27891..29306
                     /gene="ccoG"
                     /locus_tag="PLE23_00310"
     CDS             27891..29306
                     /gene="ccoG"
                     /locus_tag="PLE23_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HMX81734.1"
                     /translation="MSQDDNKFLPDESFRDRMVTIDEDGNRKWIYATKPKGKFYNYRT
                     YVSYLFFAILVVLPFIKVRGNPLFLLDVLNTRFIIFGKIFWPQDFFIFGLGMIAFIVI
                     IILFTAAFGRLFCGWICPQTIFMEMLFRKIEYAIEGDAPKQKRLNDGPWTNEKILKKT
                     AKHVIFFALSFFISNLFLAYIIGVDELKLIIFDSISNHIGGFTAILVFSFIFYGVFAW
                     FREQVCTVVCPYGRLQGVLLDQNSMVVAYDYQRGEPRGKVKDKDILNLGDCIDCNLCV
                     KVCPTGIDIRNGTQMECVGCTACIDACDEIMEKVNRPLGLIRYASENGILNNEPLRYT
                     PRMKLYTLLLIALVALLSILLITRKDVDATIIRAQGMLYQEVGTDSLSNLYTIKMVNK
                     TIKNLNVSLKLEGIPGQIKMAGDKDIPVHKEGQGSGSFFIILPKNQVKDRKTEVNIGI
                     YSNGERIDVLSTNFMGPIHVN"
     gene            29319..29753
                     /locus_tag="PLE23_00315"
     CDS             29319..29753
                     /locus_tag="PLE23_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMX81735.1"
                     /translation="MNWGRSIIIAFILFASFIFYIVYSSMNTHFDLVSENYYKEELEY
                     QKIIDSKQNANNLKEKIAIKVENNNIKLILPPELRSNTASGKIHFYNVTDARTDQQID
                     LVVNVQGEQDFERNKFVPGNYVAKISITSSGTDYYTEIPVKI"
     gene            29758..30468
                     /locus_tag="PLE23_00320"
     CDS             29758..30468
                     /locus_tag="PLE23_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMX81736.1"
                     /translation="MIQVIAPGILFGLMSNFHCIGMCGPFALSLPIQNKSTLYKWLSI
                     LAYQSGRITTYSTLGAIFGYFGRTITISGLQQSISIGAGILILLTIILPRLGTQLQLM
                     LPVNKILTPLLGYVGYYFKNQSVGGFFIIGNINGLFPCGMVYLAIAAAFTTGDFVSST
                     LFMAGFGIGTLPAMLTVSIMGQWMSQRLRHKFNKLTPYFFAIVGVILILRGLGLSIPY
                     VSPALDVPPIGVRDAECM"
     gene            30688..31131
                     /gene="rplM"
                     /locus_tag="PLE23_00325"
     CDS             30688..31131
                     /gene="rplM"
                     /locus_tag="PLE23_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMX81737.1"
                     /translation="MNTLSYRTRSVKPQDADIKWYVIDAEGEVVGRLCSRIASVLRGK
                     NKPSFTPHMDTGDYVIVVNADKVRFTGNKMQQKEYISYSLYPGGQKRKKATQLMATKP
                     LAIVEKGVKGMLPHNKLGDAIFKKLFVYAGPEHPHAAQKPEPFKF"
     gene            31137..31523
                     /gene="rpsI"
                     /locus_tag="PLE23_00330"
     CDS             31137..31523
                     /gene="rpsI"
                     /locus_tag="PLE23_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMX81738.1"
                     /translation="MEMINAVGRRKASVARVYLSKGEGKVTINGKEVKEYFPQLHIQH
                     AVTDPLAAVEVAGIYDVKVNVQGGGFKGQAEAVRMGISRCLVAINEEFRKPLKERKFL
                     SRDSREVERKKFGKPKARKSFQFSKR"
     gene            31562..32293
                     /gene="rpsB"
                     /locus_tag="PLE23_00335"
     CDS             31562..32293
                     /gene="rpsB"
                     /locus_tag="PLE23_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMX81739.1"
                     /translation="MNIPSNQEMLDSGVHFGHLRKKWNPKMAPYIFMEKKGIHIIDLN
                     RTSDCLEKAAHAARQIAKSGRKIMFVATKKQARDIVTKAAQSVNMPYVTERWLGGMMT
                     NFATIRKSVKKMQNIERMLNDGSATNLTKKERLTLTREKNKMEKVLGGIVNLNRLPAA
                     VFIVDIMHEHIALTEAKRLGFKTLGMVDTNSDPNMVDFPIPANDDASKSINLITSTIT
                     QAIKEGLEERKDSKMEAREGEDAEA"
     gene            32333..33184
                     /gene="tsf"
                     /locus_tag="PLE23_00340"
     CDS             32333..33184
                     /gene="tsf"
                     /locus_tag="PLE23_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMX81740.1"
                     /translation="MSELVISASDVKNLRDQTGAGMMDCKKALIEAEGDFEKAVEVLR
                     KQGQKLSVKRADREAKEGAVIALVSHNKNKGIVVRLSCETDFVAKNDGFVELTKQIAE
                     IALKETPATIDDLLNCSFDGKISIAEKINEYLAAIGEKIELVSYESISTNAGEGQVLS
                     YVHAGNRAGVIVALNLEGPQYEEAGRNVAMQVAAMKPLALDKDGVDASIVEKEIEIGK
                     EQARAEGKPEAMLEKIALGKLNKFYQENTLNSQTYVKDGKMSVAEYLQSLHKDLKVTA
                     FKHVALG"
     gene            33270..33983
                     /gene="pyrH"
                     /locus_tag="PLE23_00345"
     CDS             33270..33983
                     /gene="pyrH"
                     /locus_tag="PLE23_00345"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMX81741.1"
                     /translation="MPVKYKRVLLKLSGESLMGEKNFGIDQSMLVHYAEQIKELVQAG
                     VQVAVVIGGGNIFRGLDAEKSGIERVTGDYMGMLATVINGMAIQSSLEELGIYSRLIS
                     AIEMKEIAEPYIRRRAIRHLEKNRVVIFAGGTGSPYFTTDSAAALRANEIDADVILKG
                     TRVDGVYTADPEKDPQAVKFDKITFTNAINLGLKVMDMTAFTLCKENNLPIIVFDINK
                     PDNLMRIINGESIGTLVEG"
     gene            34089..35276
                     /locus_tag="PLE23_00350"
     CDS             34089..35276
                     /locus_tag="PLE23_00350"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HMX81742.1"
                     /translation="MPLYRQVGTIPPKRHTVFRQNNGQLYHEELVGTEGFSGISSLVY
                     HLYPPTLVKAKGEPYSVKPEIAVEDNLQARSFVGFQLQPEHDYLTSRKVLFVNDDMHI
                     GLAAPTKGLTDEYFKNADADELLFIHEGSGVLKTMYGSLRFEYGDYLVIPRGTVYQIL
                     FDSETNRLLFIESFSPIETPTRYRNQYGQFVENSPFCERDLKLPTDLETHDEVGDFHI
                     KIKKRGLIYPYTYETHPFDVVGYDGCCYPYAMSIFNFEPITGRIHMPPPVHQHFQGNN
                     FVVCSFLPRMYDYHPDSIPTSYHHSNIDSDELLYYVDGDFMSRNNISKGQITLHPQGL
                     PHGPHPGAIERSIGKKQTNEVAVMIDPFRPVKITTEARKLELEDYYQSWIVKPLESKM
                     TSV"
     gene            35296..36447
                     /gene="hppD"
                     /locus_tag="PLE23_00355"
     CDS             35296..36447
                     /gene="hppD"
                     /locus_tag="PLE23_00355"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HMX81743.1"
                     /translation="MDNLTTLKNYSYSGEKIFEKAQDFLPINGTDYVEFYVGNAKQSA
                     HYYKTAFGFQELAYCGLETGVRDRTSYVLQQGKILIILTTPLVEESDVARHIAKHGDG
                     IRVIALWVDDAYDAFEQTVLRGAKPFLQPETLEDENGTVRRSGIHTYGDTVHIFIERK
                     DYNGIFLPGFEKWETEYRPSPMGLKYIDHMVGNVELGKMNEWSDFYQNVMGFANLITF
                     DDKDISTEYTALMSKVMTNGNGYIKFPLNEPAKGKKKSQIEEFLDFYNGPGCQHLAVA
                     TDDIVYTVSEMRKKGVEFLYVPGTYYDSVKERVGIIEEDLEILKKWGIMVDRDEDGYL
                     LQIFTKPVEDRPTLFFEIIQRKGAKSFGKGNFKALFESIEAEQARRGTL"
     gene            complement(36880..>38392)
                     /locus_tag="PLE23_00360"
     CDS             complement(36880..>38392)
                     /locus_tag="PLE23_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMX81744.1"
                     /translation="KSEPQIYANFITEEILKQHLRVLTADSLEGRETGTPGNDKAARY
                     IARQFANLKLDAIGDNQSYMQGIYMTSYKWDHIRLEFNGGDFRHLFDYIAIPSKNKDL
                     KLNYNEIIYIGYGIDDSKYSDYKNVDVSGKVVLIYEGEPMHKDKYVITGSKTPSEWSA
                     QDNALKLKTAKEKGAAYVLIVKAGIQKMFETYRGAILGNEMVPTAVGNTVDSNIGHAY
                     ISTDILKDILGSQSANFIKQRNKAIKKAKPLSMSFKGEYRIDQKLSFKDVKSNNILGY
                     MRGSDPKLADEVLIITAHMDHLGKRGDYIYHGADDDGSGTTAVLSMARAFTKAAEYGF
                     RPKRSILFMLVTGEEKGLYGSKYYTTFPVLPLENTVADLNIDMIGRIDPKHIQSADSN
                     YIYIIGSDRISMELHELSESVNARFSNLTLDYTYNSPTDPNRFYYRSDHYNFAEKGIP
                     IIFYFSGVHEDYHKPSDTIDKIHFNLLRKRAQLVFYTAWEIANRPERLKIGTN"
BASE COUNT        11397 a         7073 c         7996 g        11926 t
ORIGIN      
        1 atgatatgtt gtcctgtacc tgctgttttg gggtcaaata tgccaccaaa ggcattgatg
       61 ataccattgc ctacccaagt tgaaaccgga accgaagcgt tgagtttgat cggtgtttct
      121 tcggcacaaa gcgatattga atccaatacc ggcagtggat ttatgttaaa gaacattgtg
      181 tctgtacagc cgttattgga atatacaatg gtgttgagtc cggtgacagc ttgttttggg
      241 tcaaaataat acccgttttt agggtcttta tcttttatat acacataata tacacccgga
      301 cccgaccacg agccaccgtt aggttttgcc tgaaagtcat catttagtat tttgtaaacg
      361 tcatcataag ggcagaaaga aattacagat ttgttgttga tcacggtatt gcgtatgtag
      421 gcaatacggt tatctgaaca gccattgaca gtataagtca aggtaatatt ctggccatct
      481 ttaagtagtg atggatcgaa caagccactg agtgtgtcta taatgccagg ccctgaccac
      541 acgccaccta caggtcccca gctacccggt acaataaagg gtccattgtt ggtacaagcg
      601 acaaaatcat attttgcatc aaccgggaca atatagatac tcatcgagtc ttgacaccca
      661 ttgatatgat aatgcaattt gttttcaccc ggcaaggcat ttgctggtga aaaccagccc
      721 cagttagcat taagaccggg tgaagcatcc cagtatccgc cccacgggct gacaggtatt
      781 gaaaaatcgc ttaaggagct acaaatggta atatcagcgg gcattacgag gtttgcaacg
      841 ttcacttttt tagattggct acaaccgtta ggagcagtat aggtgataaa ataagtcccc
      901 agcgcagaag gatcaaaagt accatttggt aaaatattct gacccgacca tgtaccccct
      961 actggtgagc caccacttac aataaatggt gcagcacctg ggcaagaagc gtcgtcatga
     1021 tttatccata taggttttac tgttatattt acggtatcct tacagccatt cttatcggta
     1081 taaataatct ggtctgaacc ctgccagata cgccatggct cataaagtcc gggtgttttt
     1141 ttcttgtcat ctatgccgtt gcactcccat gtaccacctc ctggtgtagc cccaaggttg
     1201 atgctaggtt cggactgaca aattgtaaga tcaagcggcc agttgataac cggaggagta
     1261 tggagttcga atgtaaatgt agctactgcc ggttttgacc ctataccgtc agtcactgtc
     1321 acggaataag tcaatggcgg aacacaaaca tcaactgcag atgtatttgg aaaagtcggg
     1381 tcactccatt taatattata ggtggatggg tcaccaccag atacgttggc atatagtttt
     1441 aagcattcac ccgggcatac cttggtctta ttaccttcgg gtacaagctc tacgtacaat
     1501 gggcacccgc ttacattgaa atttgcagtt gccgtcagta tatgtggctt gttacagaca
     1561 tcaaggaaag tagtggtaaa attgacagta taattgccgt tcactttgat ttccggactg
     1621 aaactaagta aaatttcatc ggttttgcca tttacacaac ttaccggagt agcagaaaca
     1681 atatcaggac tctgtggacc gtaaagcaag aatgcgcccg gataaatact gtcgcagctt
     1741 acgggctggc tgagttttaa tttaaaggaa cttgattcac atggtactgt acctgttacc
     1801 atcatctgag gtggtaaggg gtcatctatt tccaccttcc agctaccgag ccagcccaga
     1861 cactggatgt tggcatcaga gatgaaatta atggtaagac aaccactggt ggcagtaatg
     1921 gcaggtggta gtactgtgcc tgaaaacggg cccccgatca taggtgaaag cgtatcgggg
     1981 ccgtcaaaaa atctgagata atccaatttt tcttccgtac agaatttagc aaaggtgaaa
     2041 attatttttt ctccgccggg tacacatatt ttaaatgtat aattttcatt attatcatac
     2101 gtatcgggca acattccatt gtcactatcc atgagtatgc cttcgcactc attggtagtt
     2161 gcatttgaca tattgaaact tggttgagcc actacaaccg ttattgaaat gaatgccaaa
     2221 aaataagtga agaccagtcg gattcctgtc atagcttatt atttatttat agtaatctct
     2281 tttgtacgga taacgttgct ttttcgtcct gcacgatcta tcatccagag ctcgaagtag
     2341 gttttttctg aagcgctgtt accaagtaca aatgctccat taagtgtgat attgagtgtg
     2401 cctctgatac ttatctgtga acctatcggt gccaatggtg caacataata ttcatctgct
     2461 ttgtcaagcc ggttatcctt tatgaataaa gagttgatgt cggtatcaat gttgccaaga
     2521 tcgccatctc cgtcttcata ttctatcaag agctcaagtg gatcctgaaa ttctttaata
     2581 gtgtctgatg atatttttaa caagcgaata gacggtgtag tggacagtgg caacaaattg
     2641 tcatctttct gacatgaaag catcattgag agaatgaata aaaccaggac tattcttatc
     2701 atcctatgat tattttaaat gaaaaataat ttttaatata ataaacgcca tcattggttg
     2761 gtatttcggt ttcaatacca tcagaatctg taacataaac cctgaaaaaa atggtttctc
     2821 cggtttttgc ataattctgt ggattgagtg aatatttgtg gtaatagtgt acattattgc
     2881 cataataacc tgtaaaatca acaggcgtac tcaaaacttc tagtggaaaa gatgtagcat
     2941 tggcgaaatc gtcctgactt ttagaaaatt taatccttgc attcgttagt tgatcagaag
     3001 gtgttttgtc atctgagaat gcaaaatata agtcgagtga ggtttcggtt tcttttactc
     3061 ttaatgcgcc ttgcccactg tcagcacggt cgagccaggt accggttttg ccaagtacgc
     3121 cctgcatttg tggcactttg ttggtcagac ttggtaagtt gccgaaaata tcttttgcac
     3181 caccgtctat ccatgccttg atattggcta tgtattcatc tttctttgaa ttccagtcag
     3241 aacccgggtc aactaccaat ggcataatac ccgactgtcc gtcaatatca gcggttaacc
     3301 tcttataaag gacagattcg gcgctgcttc cgggttttac tctgtatgtg aatgaatttt
     3361 gaggatcatt ttttatgatc gggtgataca caagtgtgtt gtatgtactt gaaatggttc
     3421 tgaagtcagg ttcaaaggtg ccgtcatggc atcctgaatt ggcacatgta ggtttgaaaa
     3481 tatatgcgtg cagaccggca aaggattttg ggtcgggttt gtacaacgca acagtatcct
     3541 ggttgttagg ttggaaatct ttatacggat ttggtgcttc tttttcacat ccggcccaca
     3601 tcacaccaaa caacacgatt aacgaaaaaa ttctggtcat atcggcatta ttaaatttgg
     3661 tcaaaaaatg attttgctac cggatctatc agaccttggc tgagtacatc gtttttgaag
     3721 cccagcaatt ctgctattgt tggtattata tcagaactgt cacctaccgg attgccctcg
     3781 ctacctatgg tgaggtttga tggaataccc ggccctacca tcatgctgaa tattcttctt
     3841 gaattctggt cgctgtggtc gtaggcaaac cagtcattct catccgttat cggattgtgg
     3901 tctaggttgc gcccacattc aggtatgata agcattgcag tttttccgga catttcaggt
     3961 atctgggttt gtattacgtc ccagaggtgt cctacaccat gatctgcccg gtgtaatgca
     4021 cccaagtaag ttgtaaagtt gccatggcat acgtcaacat cattcaggtt aataactgtt
     4081 agcttaggtt tgaaatattt tagcacctca gtagcaaagc ctatggtgat gttgtcacca
     4141 ttatcagtta ccggagggaa tgcaatctgt tgtttatcaa gtcggtcaaa tgtttttctg
     4201 ataaaatctt tgatgctttc tctttcttct tcggtattgc cgagtgtttc gaggtggctg
     4261 ccttgtagcc tgaaactctg gttcaggaaa gtctgcattt tatgtatcgg gtcgagttca
     4321 tcatcaggat gatagatttt gaatcccttg atatattttt cagcaccaaa cgtaacgatg
     4381 ggagcaatga agttagcgcc gtatctttct ccaaagcctt catagttgga gtggttgagt
     4441 aaaggaatag agccggaaat accattacct acgaaccagg tgtctgttgc tttaagcccg
     4501 ccaaaccttc tggcatattc gaagatggtg gggtgcagtg gtctttgttt tagaccctgt
     4561 gtcacgctgt aatttccgga tacagctgta gcgagtccat tgtaatgacc ggtactacct
     4621 tttgagaatc tgagttcttt gaacagagta ccttgttttt ggagtgaagt aggcaatact
     4681 tttgatattg gttggccacc tttttggcca cccggcaatg tagtgccata ggctacctta
     4741 agtgtcggag catcaccttc aagcatattg tacataatgt tgccttcgat gttaagtttt
     4801 tggctgtccg aaaggtagcg ttgttgtaca gattcttgtt ggcgtacacc tccggcaaat
     4861 aatacgagta cgacgtgttc ggccaatggc ggattggttg tagcaaatag acggccacta
     4921 ggtaaaatat agggcgcagc taaaacaccg cccgtagcca atgccatttt ctttataaaa
     4981 ctccttcttt tcatctatta aggctgctat taatttttaa taataatcat attctgccga
     5041 tgtggcaaat gcaaagtata tgtgttcggg tgttatgttc gggtgcgatt tgataaaatt
     5101 cagaaaaaaa gctttttctg cttgtgtggg ttctcttacg aaaaatcttt tataagtatt
     5161 aatgataaat ttctcggggt cagctttcat ttcgctcaac ggtggcaatt tgatgtcagg
     5221 atcattcatc attttgctta tgattatttc gtgtgccacc tgtttgtcac ctattgattg
     5281 aatcacatta gaaaggtcca ccagtttgtc aggtgacagt gctttttggt aaaggttggc
     5341 atatacgatg ttcagatact gttcgttggt tttttcttta ttcttgtcgg cattgttagg
     5401 gagtatatct accttattta cctcgtaaat gtatttatca tctttgcagc cgaccaatac
     5461 aaacagtaca atgatgaaga atatgttgaa tgttttcatt ggtaatactt taaaagttag
     5521 catattcatc ggttatcatt attttttcct gaatctttcg aatgtcacgg ttgaggatgt
     5581 attcgccgag tatttcactt gtttcctgcg actttggttc tcttgacaat aaggtacggt
     5641 aacaccaaat gatcatacct tcaaaaaatt cgttggtatc acagatggca tctacgtatt
     5701 cctttttgtt agtgcataat ttgttgaaga atagattggg tttgttgtat tctacgatat
     5761 caaaacctac cagaaattca gtcgatgtag ggaaacgcca tagcaaatct tcaaatgttg
     5821 agttgacaaa attgaaggtg ttcatgtgga taaaatcata gatagagtta ttgaccatac
     5881 gactaaaaaa cacatcgtaa ggtatctgac catcaaaaaa ctgcactttt gtatccaaaa
     5941 tagactgaag attatttttt tcgagcaaac ccttgttgta tgattcccag tcaccgagta
     6001 gagaatcttc cttcatacca aagactatgt tgccaatgcg catattgata tcttcgtcag
     6061 aagcgccttc tatggtgcgt acttttgcca gattatacat atattgcgaa taagccttac
     6121 ggtacgagcc atcaccaaat acagaatcgg tactgttctg aagcttttgg atgatttgtc
     6181 ttcgggcatt gacagaaagg ttattgctgc gaagcagatt gacattttcg tccatttcta
     6241 catcaagtgg ttcgcggcca accagatcaa taaacatcct gttgacgtag ttctgcatct
     6301 tgatggtaga tattttggta tagtcaggtg ggttgttgcc accgattatc tccgggtctt
     6361 tgttacatcc agtgagcaca acgactacca atgtaaagag ggtaatgaat aatttattca
     6421 ttgtggttgg ggttagttaa tcgcgataaa gttaataaat ttaattaatt cgaatataat
     6481 taattaaaat tatttatttt gaaagttgtt tttttacttc ttcagagatg gctttgccct
     6541 ctgcctggcc tgcaagtgct tttgatgcaa ggcctataac cttacccatt tctttcattt
     6601 ctgaaacacc attatcagct atcaatttac taataaaggc gctaagttct tcagcactta
     6661 gctgcttagg caaatatttt tcaataacgg caatctcctc ttcttctaca gatgccagtt
     6721 cgggtcggtt ttgttgcctg taaatttcga gagaatcttt tctttgcttt acgagttttt
     6781 gcagtagttt gatttcattt tcttcattca gttcttcttg ggtgccacta gttttgaaca
     6841 ggagtattgc agacttaaca gctctgatgg ctctcagtgc tgcctggtct ttgtttctca
     6901 tagcttcttt caggtcttcg gtaattttag cttccaggct cataattttc tatatttatt
     6961 gttaagttaa taaagtcttc aagactaagt gtttctggcc ttttctgata aaaaggctca
     7021 tttaggatat gctcggggag gtattgcttt agggtatttc taatcatttt acgtctttgg
     7081 ttaaaagttg ttttgaccag ttcggtaaat agtctgttgt tgtattgcgg tccggtatct
     7141 tcttttcttg tgagaataat cacggatgaa tctacctttg gtggaggatt gaagtcggac
     7201 ggactcaggt gaaaaagtag ttggccctga tagtatgctt gagtaaggat agatattaca
     7261 ccataggttt tgcttccggg tccggccact attctctctg ccatttcttt ctgaaacatt
     7321 cccaccatta caggtacata ctctttgtaa tccagcattt tgaatactat ctgtgatgat
     7381 atgttgtagg gaaaattacc aacaagccca aattcttgag cattcatcag ttgtttgaga
     7441 tcggttttca ggaaatctgc ctcaactaca ttgagtagag gaaaacgatt gtgaagatgc
     7501 gccaccatat cgtggtctgc ttcaacagct gtgagtgaag gataggtact gaggagtttt
     7561 tcggttagaa cgcccatgcc ggggcctact tccagcgctt tacctgtggc atttattgca
     7621 ccaaattttt caacaatcct gtcaactgcc attttattga tgaggaaatg ctggccgaaa
     7681 gacttctttg ctttcatctt ctttgtttcg ataattctat tttaaatcat aaaattaagt
     7741 gttagtagct taactttgct gacttattaa agataaatca ataatttata ctaaatggat
     7801 acaataaaaa taggaataac agttggtgac atcaatggta tcggtcttga agttattctt
     7861 aaaacactca gcaataatct tgtatataag aattttgcac ctattatata tggctcacca
     7921 aagattgtct cttaccataa aaatattatc aacctaccag acctccatat caaccaggtc
     7981 aaaacacctg atcaggcaca gcaaggtgtg ataaacctta tcacatgttg gcaggaaagt
     8041 gtaaatattt cattaggtca ggcaagtgcc gaagcaggaa aatatgcctt gataagcctg
     8101 acagaagcca ccaaagacct gaaggcaggt gacatacacg ggatagttac agccccggtt
     8161 gataagcagg taatccataa cgaacagttt ccttttatag ggcatactga gtattttgcc
     8221 aaggagttca actctcctac tgtgatgtta atgacatctc cgaatgtatc agtaggcatg
     8281 gttactgacc atataccact caaagacgta tcaagatccc tttctaaaga tttgataatt
     8341 gaaaagataa aaatcttaca taaaaccctt aggcgtgatt ttggctacga gaaagggaaa
     8401 attgcggtat taggtcttaa cccacatgct ggcgataaag gattaatcgg tgatgaagaa
     8461 ctcagcatta tccggcctgc tattgtagag tgtaaaaatc agggtatttt tgtatttggt
     8521 ccatactcag ctgacggatt ttttggatcc gctcaattca aaaaatttga agcagtgcta
     8581 gcactttacc acgaccaggg ccttataccg ttcaaaacca ttgcatttag tgaaggagtt
     8641 aattttactg cagggctacc agtagtacgt acctctcctg accacggtgt aggatacgat
     8701 attgcaggcc aaaacgtagc agatgaaaca tcattcaggc atgccttaca gatggcattc
     8761 gatgtagtca gaaacaggcg cagttatgat gaagcacaca ataatcctat tgcaaaaaga
     8821 gccaaaaact tcgaagacga agtactcaag gatgagaatt aatttggcgg tgcaatagcc
     8881 gctgtgttat tctgcaatcc ataggtgccc tggtaataat ccggcagttt tacagaagta
     8941 agctccgtat tatcactcga tgtggccttt tggttgttaa taaaattgaa ggtaaggtaa
     9001 agttcgtatg aattttcagt agaagtatcc atttcatcaa acacattcag attgaaacgt
     9061 tcaataaggt ctatcagtgt tttggcatag tgtggtgcag tagcatagcc acaatccttg
     9121 agcccggttg cccatgcttt ataatctttt ttatcataag aaaacagccg tgcatatctt
     9181 ggattggtat tcaggaaaag cgaatgatcg gcaaatgatt cctcaggtgt atcatacttt
     9241 ctgaagcaag acagtatcag gttgccaagt gtatccaagt cgtcatcttc cttgtaatat
     9301 actttaccaa gccattggtc tttgcatttg ataccaaaat ggttgttggc gctgtcggcc
     9361 agcatcccgt atcctgcatt cgactcaaga atgccctgag caagtgtaat acaagccggg
     9421 atattgtatc tggtctgatt ttcaagagca atatgatgat atttgcggat ataattgttt
     9481 actttttctt tgtaagactg agctaaagta aggtttgtaa atgccgagac gatcagcatg
     9541 aatgtcaata tccttttcat ggttgttatt gtttacttat tcaataaaca catatactga
     9601 tattttatat atatgtttca gtatatttaa ataattgttt ttccgttact attctttttg
     9661 gtaatttatt aaatattatt tttgccgcat gaagaaatca tacttagtac ttgttatatt
     9721 atttctggtt ttgttaatag accaagccag taaaatatgg gtcaagaccc acatggaata
     9781 cggtgagcag tttgcaatat ttggtttatc gtgggccaac atacattttg tagaaaacga
     9841 aggtatggcg ttcgggatga aattgggcgg tgattacggt aagcttgcgc tcagcttgtt
     9901 caggatagta gcagtaggat ttttggctta cctgataatc aatatgataa aggagcgaaa
     9961 atccgatttt gtcctggtat gcttcacttt aatactggca ggtgcattgg gcaacatcat
    10021 agacagtgca ttttacggta tgatatttac agagactcct taccatagtg gtggtgttgc
    10081 tactatgttt ccgccgggtg gtggctatgc acctttccta catggcaaag tagttgacat
    10141 gctatatttc ccattgacag aaggggagtt tccgtcatgg gtgccatttg taggtggcga
    10201 tcactttcta tttttcaggc ctgtattcaa cattgctgat actgccatta caacaggtgt
    10261 attactgctt ctgttgttca gaagacagat ttttcactga tggaatagtt taattggtcc
    10321 atactgtatt atagtataga tgcccgattg atatgtaatg ttgtaaatgt acagccttgg
    10381 gaggcatttg ggtaagaaag aatagcaata gaaacaagag tgaagcaggc agtgtaaatt
    10441 attcttctat cagagtctgt tttacttgtt ttgctaaaaa gttgttcact gcaataaccg
    10501 aattttctat tcttgggtag ttaaggctgt agaaaatgta ttttccggtt cgttttgttt
    10561 ccagaatacc tgcatctctg agtattttca gatgttggga ggtaatggat tgttcgagct
    10621 tgagtgagtt gtagattttg ttgacattga tgcgtttgtt gctgtcaata aactcgacta
    10681 tcttgagacg cagtgggtgg gtgagtgccc gcaatatctc tgatgacaac tcgagctttt
    10741 cgttgtcgat gtttaccttt gtatttttca tggttgttgt gtaataattc gaaaagtatt
    10801 aacacggcaa aggtcagtaa ttttttttaa attatcaata tttacgaata aaaaatgctg
    10861 taaaccaata agttcacagc atttttcaga acacaaattc aaaatacata taaatactta
    10921 tacagtataa tgtgttgtgt atcttttttt tgggtggtat tttttgatca aactcagatg
    10981 ttaagacagc ataaaaaatt aagcggctaa ttcttctacc aatcgtgaaa tttgtgataa
    11041 tctttctttg tcgattgaat aatagatgaa tttgccgttt ctgtcagtga tgactacacc
    11101 tgctcttctt agtatggcga ggtgttggga agcaactgat tgttcaagtc ttagttttat
    11161 gtaaatatct gtaacggtca gactatcact ttcttcgagt aggtcaatta tattttgtct
    11221 aagtttatgg ttaaccgcac gcaaaaccaa aactgctttg cgtagctcgg cataatcgag
    11281 ttcaatatcg tttttgcctt tttttagtaa aatcagctcg tttttttgct ttctcatatc
    11341 tagattgttt tagaatatta ctcataaaaa tatcatgcca aaattatacc aaattgtaat
    11401 atttaaaaaa aatatcaact ataacttacc tgattatgtg cacaaaataa aaagattttt
    11461 tttaaattga taaatatttt tatttgtaat actaaaatat gttcttcggg gtagttatat
    11521 tcggtgattt gaaataatat ggtacactgt atgctatatc caataacgtg ccattgttat
    11581 acttctcgtg gctgatggtt gccatcatgg ctgcattttg tgttatttct gatattttac
    11641 tactttcggg taaataagat gttccggata atttttttgc tccatcaccg gtgcacaaaa
    11701 tctgtttccc tgcataaaga ctgaaaaaag cttcatccat ggttacaaat tcatcttttt
    11761 cgaaatgatt tgttattgca tcatatattc gcgcatacac atccatgcgt cgggcatcca
    11821 ccatacacat gatagtatca taagccgatt catcttcgac cgatttgtat agaatttcaa
    11881 acgtatcagt gccaatcaat ggaacattaa gagcataaca gatagcttta gccgccgata
    11941 accctacacg aagtgatgtg tacgaaccgg ggccgagatt aattgctaca gcatcaatgg
    12001 agtggagtgg tatttgtgct tcattagcag ctgattgtat cagtaaggtg atttcaccgg
    12061 atatattgag ctctggtgac aaagttttaa atgcgataac ttggctgtca acacttattg
    12121 ctaccgaact ctgactcgtg ctggtttcaa tgttgaggat tttactcatt agggtttact
    12181 tgtgttatgc cggccaacaa gccttttaac cagtagttta cattttagat aatgaaacaa
    12241 atcaccacaa aaggcactga aaagaccacc aaaaaaactt aaggcgatgc ctatttttaa
    12301 ataaaactgg tcgggactgt taaaagattt gtattgaaat aattctacta ttaacaatag
    12361 agctgttatt agcatcaata tcaccgaagc attgtgcaag cgttcgatgt atctgggtgt
    12421 aggcgtggct gtgatgtgaa aaagaagatt gattgttgcc tcatctggtt ctttgatctt
    12481 tagcagttcg ttcatgattc ttttggcttc atctgtatta cctttcaaaa acttaacttg
    12541 agccttatgg aacaacagaa ttgaataaat ggtctcaaat ctttctttct gcctgatatc
    12601 cagtgatgtt aatatggcag tgtcgagttt ttggtctatt tcatcatatt cattcaactc
    12661 aagcaacgac tctatatata taatgtagca gtgtagatat ctgtagggct ccaagctttc
    12721 aaatagtgtg tgaaatttct caaaatacag aattttctcc tcatcttttc tgtcgatgag
    12781 gttaatgtaa ctgctgtata atttatcagg gtcctgtatc atcctgagac tattctttat
    12841 ataatttata gagtactggt ttgccggggc ttgcgtcaat accaaaagag gcaatctgca
    12901 gattgagcaa ataaagaccg tctttggttg tgttatctat ataaaccaac tctgtgatgg
    12961 ttttgttcaa atccagtgtt tcgggatatt tccagaaact tttgtggccg gcaagcttac
    13021 cgccgtcttc ttctctgtcc accgaaggca aatctaccaa taaatggcga atgttgcacg
    13081 aatttaccag ataagaaatg gcatcggcag ataaatatgg cggattggta cctgagtaat
    13141 ggaatgtttt tttgacatca tcgtttggta gtgtacgtat tacgagggct tcacattttg
    13201 tttcatttgt aaatacagcc cgtaaaatat cttctgtaat aacgcggtct gcgttttctg
    13261 atttttgagg aaagacactg ataagctcag caataaagtg gaattgccga agtgtgtcat
    13321 tgatactgac atcttctact gatatatgac cgacacattc ggtatgggtg ccgttgccat
    13381 gtgggttaat ttttacattt ttaaaattga cagcgccacc ctgtttggtt gacccgacaa
    13441 aattgcccat cactacaggt tgtgcgtcaa aaaccggtgc ataaaagcaa ttaacacccg
    13501 gttggtcggg aagtaatggt aaagagatgt ctgtaccggt tgagaggtca gctgtataat
    13561 gaataccatt atgtgtaagt ctgatgatca tgttctattt agtttctggc atccttgccc
    13621 cagtcgagtt tttcacgtat ggtctgcatg aatccgttat cgttgagtgt tacagttttg
    13681 atacaaaagt cgcatttcct taccgcaaac tgatgctgag ctgtgattga ttcaaaacgc
    13741 gaatcgagtg ttaataggaa tgtgtcggca cggccctcaa cctcgaaaga aattaccgaa
    13801 ttatcagaaa tgacgatagg cctgacattc aggttgtgtg gtgctaccgg agtgattaca
    13861 aaattgcctg aatccgggaa aatgataggc ccgccacagc tgagcgaata accagtagag
    13921 ccggtaggtg ttgagatgat tagaccatca gcccaatatg aattcagaaa agatccattg
    13981 atataggttt tgatgcgtat catagccgat gtgtcgcgct tgatgatggt acagtcgttg
    14041 agggcaaatg tgagcccgtt aaataaagct acgctgcttt ccagatgcaa gagcgtacga
    14101 ttttcaagtg aatacatacc gcgctttaat tgtttgacag attggcgtag atatttgctt
    14161 tcaagacttg ccagaaatcc tagccgtccg aggttgagcc ccaatatagg tatatcagaa
    14221 tcgcgaacca gtgtagtggc gcccagcata gtaccatcac cccctactga tagtaatatg
    14281 tcgggttcct gtattaccag gtcttcatat ttttcataca aatcggggtt cgactggaaa
    14341 tcaatatgat ttttgagttg attaaaatac agattatgta cagtgacctc taatttttct
    14401 tcatgaagga catcaaagag ttcttgaatg caggctttat cctgttgttt gaaaattttg
    14461 ctgtaaacgg ctattttcat aatgattaaa aactgaggtt cagatgcaaa aatatatcct
    14521 tttcgtattt ttcgttcata ctgtattcta tttgaagcat tttgtcataa aaagctacga
    14581 tttcaagacc gatgccgcca ccttttaaca gcttgtcgtt aaagttagtt tttggtacat
    14641 ttttagcgga aggtatgtag cccatatcaa agttaaaagc gagaaagagt tgaacttgtg
    14701 gttttctgag agatttaatt ggcatgaatt ttccaagatt ccagtaattg tcaaacagca
    14761 tatactgcag actggtcttt gataaagcgt attgctgtcc atcgatgatg tagtattcat
    14821 aacctcgtat gtagttgagt tcatcaccta aggcacggtt gtgaaaatag tcggtcgctt
    14881 tgtttatcca gttgttctgg tgacgtagtt gttgggttgc tgtccatttt gccgaaagtc
    14941 tttgaaagta tctgaagtca attaaggtta tcagagcatc ccggatgtta ttgatacctt
    15001 gtttttcaat cagaaaattg aatacatagc cttttgttgg atgcggttgg atgttgcgtg
    15061 tatctttggt gatgttgtat gaaatgaccg gatagctcat ttttccgtca ggagaggcaa
    15121 aaaaatcagg ctgtagtttg tcgagtacac ttttatttat acggtagtat tgccattcta
    15181 tctttagctt ttgtttggta aacaaacgag gctgatacga aagtgtagcg ctgaattttt
    15241 ttctgaaaat aatggtagtg tctaaaaacc accttaactg ttcgttttcc ggtgtagtag
    15301 cataagctac ttccttgctg ttggcccata atccggtaaa ctgaataccg agattctgct
    15361 tgcgattcat atagggtagc caatatgatg cttcggcctt atcatggaaa ccgagatgaa
    15421 aagtaccatc gagatggtcg cggttgccgg tcagattgct gatatacagt ttgattccgg
    15481 cattgatgcg tgccaggtca aaatggtaat tgcgtgccca cacattgaag tttcggtcca
    15541 ccatttcgaa ataaggtact ggatagatgt gccagctttc atcaatatgt ataagtacat
    15601 tgatggcgtg tggtttggtt ttggcatttt cgtatgtgat cttcacctga gaataaatgc
    15661 ctgtattcat cagaaagagg cggttatcaa ccaatactgc ttctattttt ttttctacca
    15721 gggtatcacc cggtttgata tttatatcgc gcagtactat ttctttgttg gtacggttga
    15781 gtccggtgat gacgatagag tcaataacca cactctgcgc atacacactt gtggccggca
    15841 gaaaaatcat taaagcaaaa caagaaaaga aaatgaatgg tattaaccgc acatctttac
    15901 gttaagtaat gtgcgaaatt acacattaag gtatgacatc aggctatcga atctgtcttt
    15961 tatcctatct gtataatcat tttcttcgta agtgacttta atggtgtatt ggtagcgttc
    16021 aaatgctgca agtatagcac ttatattggt agtattgatt ttgatggcca catcgataag
    16081 ttcaacatta tccgaattag ttatcagcag gcctatgact gaaaatcctt cattttcaac
    16141 aattctggat atttcggcga gtgaataatt gcgtttggtg gtttcgagta cgatgatgct
    16201 gccgggctcg gtgatggatt cggcttgtgc aaaatttttg ataataccat cctgtgtaac
    16261 aatgccgaag tacatcatat catcttttac cacaggcata acagacatgt tgtgttctgc
    16321 catgtgtttg agtatttcga aaatatgatc cttacagtaa acgtatttcc ctgcaaaatt
    16381 cataggtagc gtagcaattt tctcccctgc atcacaattt agcagagttt cttccgataa
    16441 tacgccaaca agtttgcctt tctccagtac cggcaaatgc tttaccattt gctccgacat
    16501 cagttggagt gctttttgtg ctgcatcacc gatttttatc ggggttacat ttttcgaaat
    16561 aatatcttta gcaatcatcg ccaattcatt taaacattta acaaatcaac ctctcgtttt
    16621 gctacacaaa tactgttatt gttaagttaa acaaaaaaga tgcctaatgc atgtaaatgg
    16681 attttgggat aaagttaaca ggatattttt atccgagtat tttttttctt tctataaaat
    16741 attcatcatc cgtcagctcc cctcgttcac gtctttggtt gagtaatttt atttgttcca
    16801 acaaatcagg catttcttgt acttcctgct ttggtctagt tgcaggttct gtcctttctt
    16861 gaggtctggt gtagcgaaaa ttattattca ccaattcctg aaactctggc tgtacacgca
    16921 agaatgcaag gtcgtccttg gtgcgtaatg tgtcaaaatc attgtatccg ttctctattg
    16981 cttttgagag gtgatacata gatttgtctg catcttctgt cagcgaataa gcacaggcca
    17041 tattgaaatg aagcactttg tcattagggc tcagagcaag gcctttggtg tattcttcta
    17101 tggcacctgt ataatcataa tctttgtact tttcattgcc ggtttttata tacggattat
    17161 ctttgataaa gggtgtacct gccactggtt ggcggcccca aggtcgtgat tcatgcaggt
    17221 ttggctcatt gtatttttga ttaaattttt cggtggacat agtcaaaaac aacacactct
    17281 ccatcagcga ataaagccac aggacgaaga cccaatcttc cacaaacatt gacagagcaa
    17341 gaaacagtag aatcctaaat attcctcctt tgatatcgcc aagataaaat ttatgtaaac
    17401 cgaatatgcc aagaaacaga gataataatg ctgctatgta tttatttttc acgattcaat
    17461 attgtttaat atgatttaca gactaacacg gtcatgtcat cggtatattc ttgttcttgt
    17521 ttaaattcat gaattttggt tttgattttt tctactaggc catctggtgt gagattattt
    17581 gaatgtcgga ataaatcatg taccaaagca tccgaaaaat attcgccctc gttgttgagt
    17641 atgtcagtaa taccatcggt gtagcatagt actgtacaaa cttctttgta cgaaagctct
    17701 cctttttcga taaaaggcaa ttcttcgaat ccgccgagta tagtgcttcc tttatcaagc
    17761 tccagaatca tcccgttgat attagcgata ggaggcacat gcccacagtt gatgtattcg
    17821 atggtttttt ttgttgcttc aatttttgct atgaaaaatg ttatataacg gtctcctttg
    17881 gcaatgttat aaaccgattt gtttaactcc ttcacaagag ataagagctc tgtgtctttt
    17941 gaaagaagta cgtgtagggt ggcttgaaaa ttggacataa gcatggccgc cgcaagccct
    18001 ttgcccgaaa tatctgctat acagataata agtgattctt cacttaagct tatgcagtca
    18061 tagtaatcac cacctacacc tatgtgtggc aggtaataac ttgacaaatt gaaataatta
    18121 ttgcccggta gcttactagg aatgagcatg tgctgtacat cactggccag ctccatctca
    18181 cggttgagtc gttcttgctg tatttgtttc ttgaacagac gcttgttttc gatagcaacg
    18241 gcaatgatat tggttatcgt agtaataaat aatacctttc cgtatgaatc gtcctgattt
    18301 tctatatttc cgatcagtac ataggctatc gggctatttt tgtgttgtac aggaatgatg
    18361 acatcaaact gcataagacc cgggtgggtt tcgttgtcga gatggttgag tctctcgtat
    18421 ttgtgtagtt catgtgtgag cgattcgtca ataacagtat cacccgcccc aatgctggta
    18481 gcacagaccc actctttgtc tttttggatg aaaagtgcca ttttttttac gccgagttgc
    18541 cagttgatga agtctgaata catctgaaac aaaccctgtt ctttgacatt atcattgatg
    18601 gcctgggtta tactcataag tccgttgagc tgcagttgtt taagactgag cttgtgttcc
    18661 agatccgaaa tctcattttt ttctttgact ttgctttcca gaggcgacat ttgagttaca
    18721 ggtttttgtg tttagagaat gcaatttaca aataattatt tatctatctt aacatcaaag
    18781 taatctctaa gcgaatttcc aagaatattg atactcagta ctagcaaaac tatagtaagt
    18841 gccggtatta ctgcgaggaa ataataaccg ctcagagcat aaccgtaatt ttcgtttagc
    18901 atgttacccc atgatggagt tggaggttgt actccaaagc ccagatagct tagtccggct
    18961 tcaagaatga tggctgtagc aaaattgccg gcagcaatca ccaatatggg gccgatgata
    19021 ttaggtaaaa tatgacggaa catgatcctc ttgttgtcat aacccagact cctggcagcg
    19081 tcaatatata cattttgctt tgcagacatt acctgcccgc gtactattct tgccacatca
    19141 acccagagtg tgagcccaat ggccagatac agaatatgaa cgcccctgcc caatgccaaa
    19201 acaatagcaa aaactagtaa tagtgtaggt atagaccaga tggtgttgac aaaaagcata
    19261 agtacggtat cggttttacc tccgtagtaa ccacctgcca gaccaatgag aatgccgatg
    19321 gtgagagata taagtactgc tgataaacca acagtgagcg aaatacgaga gccgacaaga
    19381 atgcgactat aaacacaacg gccaaaggca tcagtaccaa aagggaatgt ctttcggatg
    19441 attttgtatt ccttattgag caattctttg gatttactaa gtgtgatggt ctcggtaggt
    19501 agtggttcgc cggtaaattg atttttgcct gtatattttt ttaaaatgat gctgtctttt
    19561 gcttcatgcc agctgaccac aggtatcaat tctgtatgga tattgctgcc aaagaatgct
    19621 ttcttaagtc tattttcagt ttgggtatct ttgttttgca agctaataaa agtcatgctc
    19681 acaaacggtg gatgcagggc tatctcaggt atttgagtat ttacatcggc tgagttatca
    19741 acagcaagaa tataacaacc aagagaaacc acaaaataca acaatagtaa agtaaccgat
    19801 aaaaaagccg atttgttgta gcttatccgt tgccagaata tattatgcaa tttggtatgt
    19861 tgattatata agtgagatta atatttcata tcaattttgg atggtaaaag tataactatt
    19921 tggcgataat ctgacaatca caatgcttat aaagcaattt tgtaggaatg gacgactgac
    19981 gaaaatcatt atgaagtaaa atgtaggtca tgtgtcagag caaataaact gttgcatttt
    20041 tgcatttcgt ttactgttcc atttttttaa aaatattatt agttgtcatt cgattaaaag
    20101 atgaatctgc acatttcaga catcagaact atagccgaag taaaggccga tttcaataag
    20161 ttgttccctt atctgagact tgagtttaat gttaatgctt cagaaagaaa tggaagaaca
    20221 acttcaaaga accggatatc tgacgatgta aaaataggga gtatccgtga tcagcatgta
    20281 gaagcaaact tcgagtttaa tgaaaatagt acagtagagg aatttgaaaa caacttcttt
    20341 gaaaaatttg gtttgagggt tcaagtgtat cgcagatcgg gtaatgtatg gcttgaaacc
    20401 aacatgacag ataaatggac actgcagcga caaaacgagc acggcaggga aataaccgag
    20461 atataaattt tatttatact gtaccggtca gaagagaaga tggttgagtt tttctttgtt
    20521 gagaatctgt atcttacctt ctttaatatc tatcagtttt tctgatttga aatcactgag
    20581 tgttcttata agtgattctg tagcagtacc tacatacctg gcaaaatctt cacgagtgat
    20641 ggatgctgtc atggttaggt tttctttgtc gaatttttcc tgtatttcga gcagtgcctt
    20701 ggctatacgt ttccggaggg agtcataagc gagcctgagc attctttctt ccttatctgc
    20761 caggtctccg gcaatgagtt tgataaattt ggtagagagc ctaaggtcac tgaacaccat
    20821 gttcagaaaa tcatcttttg gaatgagtac tacttcactg tcttctagta gctcagccga
    20881 atcggtataa ctgacattct caaacaaagg aagatagcca atataatcgc cttttgagta
    20941 aaatgaagta atgtattcct tgccatcttc attaacacgg taggtcttga gtttgcccga
    21001 ttttaggaag aaaagatatc ttggtctttt tccttcactg tataaaagtt gtttccgact
    21061 gtaatgttcc gattcgtatt tagaaatatc gagtgttaga gcacctgtat tttgaaggtc
    21121 agtaaccagg ttcgaaaatc cattattttc gcgattgtat tttttttcta ttatctcctg
    21181 tttttttaat ctggtttcta aggcatgtag tagttctata tcatcaaagg gtttggtgat
    21241 atagtcatcg gctcccattt ccataccgcg tctgaagtcg cttctttcag tcttagcagt
    21301 gaggaaaata aaaggggtgt gttcggtagt gtgattattg cgcaacatgt gcagtacacc
    21361 atatccgtca agcccgggca tcataatatc acagatgata aggtctggtt tcgagctaag
    21421 agctacatct actcctacac gcccattttc ggcttttagc acattaaaac cagataattc
    21481 gaggatttcg gcagtatttt ctctgatttc cagatggtca tcaattatta atatggtttt
    21541 tttcatttaa aaatttgata ttagaggcaa aaataagcat taagttagta agtatccggt
    21601 actattaaaa atattacatg acttttatca taaagattta ataaaagata accataaatt
    21661 gcgcccttat ggtagtaatg gatgaaaaaa cttcagtaaa attacactgc gagcattgtg
    21721 gtctggagtg cgaaaatgac cttatcaaac tagaagaaca cagcttttgc tgtgaaggct
    21781 gtaagctggt atatacatta atcaacgaaa ccgggctttg caattattat aacctgaacg
    21841 aacatccggg tatcaatcaa cgcacaagtg tcagagaaaa taaattttca ttcttagatg
    21901 acgatagtgt agaaagtcag ataatagggt tccgcaataa cgattttaca caagttgtat
    21961 tttacctgcc ggccatacat tgtagctcgt gcctttattt gcttgagaat attcataggt
    22021 tcaacaaagg catcatcaac cttgaaatcc attttgcacg caaggaagca aaaatcaaat
    22081 acaaacataa ggaagtaggt ctcagggagg tagccgagtt gcttactcgc ttaggttatg
    22141 agcctcatat tagcctcaga gatgtagccg gtgcaaagcc caaaacgtct aaatcgctta
    22201 tttacagact gggtattgca ggtttctgtt tcggcaatat tatgatgatg agttttccgg
    22261 agtatctggg ccttcatggt agcgagaagt caatgaccgg gctattccga acgcttaatc
    22321 tgctactcgc cataccaaca tttttttata gtgccacacc attttttaca tcagcctggt
    22381 caggtttgaa gaacagattt cttaacattg atgcacctgt atctgttgcc attattgtta
    22441 catttggcag aagtgtgtat gaagtaatgg caggcgttgg aggaggcttt ttcgattcga
    22501 tgtcgggtat catcttcttt atgttgctcg gccgggtatt gcaagacaag acctaccagc
    22561 atctcgaatt tgatcgtgac tatacttcat attttccaat agctgtcaca actatacaga
    22621 aaggtgaaga agtgccggta caagtatccg ctctaaaaaa aggtgatttg gttcgtattt
    22681 ataatgaaga aattataccg gtggacggaa tattgtcaaa tggcaaggct tatatagact
    22741 atagttttgt aacaggcgag tcagcagtaa acaggataga aaccggagaa atcatctatg
    22801 caggaggtaa acaaatgggt gcagccattg atttgctgat acttaaagat gtatcgcaaa
    22861 gttatcttac acagttatgg aaacagacaa aagtaaaatc cagaaccgac caggaccaaa
    22921 agagttttgt acattatctg agcaaatatt tttcttatct tgtattttcg atctctgcat
    22981 tggcaggtat ttattggtgg atacataacc cggccaatat tttccctgca atgacagccg
    23041 tactcattgt agcatgtccg tgtgcattgc tgctgtccaa ttctttttct aacggtttta
    23101 tcatcagatt gctcggttac aacaaatttt ttctaaaaga tgcattatct atagagttta
    23161 tagccggtat aaaacatatt gtatttgaca aaacaggaac acttacagac acaagccatt
    23221 acaaggtaca ttatgaagga agtgaattga cacagccaga aaaactaagg atagcagcta
    23281 ctgcaagaca gtcaagacat ccactcagca aagcagtatc agattatctg ggtatccggc
    23341 aggtaccaac gatcatcagt ttcgaggaat tccccggcca ggggaccaaa agcatatttg
    23401 aaggctatga gataaaactc ggatcagaag aatttacagg gcaaatgcag aactctgaca
    23461 accgttcgag agtgtgtgta gtagtaaatg gagaatacaa aggaaaattt tatatagaaa
    23521 accaataccg cgagcatgca gaagaagtaa tagccgggtt aaagaaaaaa tatcagctca
    23581 cagtattatc aggagataat gataaggatg ctgccagtct taaaaaagta ttcggcgaaa
    23641 acagtacaat tatatttaac caaaaacctg aagacaaact gatatatatc aaaaacgggc
    23701 aaaaacaaaa acaaacggtc atgatggtag gtgacggcct gaatgatgcc ggtgcactca
    23761 tgcaaagcga tgtaggtatt gcaatttcag acacaagaaa taattttaca cctgccagcg
    23821 atgtaatctt gggtgctgaa agtttttcaa agttgccggc attattaaga ttatgtgcag
    23881 atggtaaaaa aataattatt ggtagtttta tactttcaat actgtacaat atagtaggcg
    23941 tatatttttc tgtaacggca cagttatcgc ctctggtagc agcaatactt atgcctgcaa
    24001 gcacatttag tattattatt ttcacttata ctacatctct tgtaatggct agatacagaa
    24061 ggctctcaac cggtgcagcg taaaaatata aaaattatca atattaatga tgacttttgt
    24121 catagcgata gaaaaatatg aagagtaatt ttgtgctaaa tttatagaca tgggtgtaat
    24181 attcatatta ttgtttgtga gtttggccat agcgttatta tttttatggg cgtttttgtg
    24241 gagtaccaag accgggcaat tcgatgatga tatatcacct ggtgtacgga tactatttga
    24301 tgaagaaaaa tctaaaacaa acaataacta aataactgaa agatggaatt agaaaagttt
    24361 tattatgaca accgacccgt aaaatggttt gcttatgcga cgattttttg ggggattatt
    24421 ggtatgttgg caggactgtg ggctgccatt gccatatatt atccggcatt gaaccttgga
    24481 tgggctccta ccacattcgg gcgtatgcga cctgtgcaca ccaatgcagt tatttttgct
    24541 tttgtgggta atggtatatt catgggggca tattattcac ttcagcgttt gttaaaggcg
    24601 cggatgtata gtgataccct ttcaaaaatc cacttttggg gttggcagtc aatcattttg
    24661 gccggtgcac ttacactttg ggcaggttac acaacatcca aagaatatgc cgagctagag
    24721 tggccactgg atattgccat agctgttata tgggtattat ttggaattaa catgattggt
    24781 accatattga aacgtcgtga aagacatctc tatgtggcca tttggttcta tcttgccaca
    24841 tgggtaacag tagctatgct tcacatcatc aacagtatcg aaatcccgat atctttcctt
    24901 aaaagttata gttattatgc aggtgtacaa gatgctcttg tgcagtggtg gtatggtcac
    24961 aatgcagttg cattcttcct tactacacca tacctgggcc tcatgtatta cttcctgcct
    25021 aaggctgcca acagaccggt atattcatac agattatcta tcattcactt ttgggcattg
    25081 atatttatct atatctgggc aggaccacac cacctattgt acaatgcact gcctgattgg
    25141 gcacaatctc ttggtacagt attttctatt atgcttatag ctccatcttg gggtggtatg
    25201 ttgaacggtt tgtttacatt acgtggtgta tgggacaaag tgcgtgaaga accagtattg
    25261 aaatttatgg tggtggctat cacttgttat ggtatggcta cgttagaagg tccgctactc
    25321 tctttgaaaa atgtgaatgc catttcgcac tttacagact ggaccattgc acacgtacac
    25381 gtaggtgctc ttggctggaa tggtttcctt acctttggta tcctttactg gctgatacct
    25441 agaatgtgga ataccccatt gtactcaaag aaattggcca acaatcactt ctggatagga
    25501 accattggta tagtattgta tgctgtacct atgtattggg ctggttttac acagagtctt
    25561 atgtggaagg catttactcc ggaaggtcag cttaagttcc agttcctgga gactgtcaca
    25621 cacattatgc cactctacat aaccagaagt attggaggtt ttctattcct gaccggtaca
    25681 tgtataatgg cttataactt gtacaaaaca ataaaggcag gcaaatttgc agagcaggaa
    25741 gaagctgaag ctgcaccact tgataaaaac tggacagctc catctaatga ttactggcac
    25801 agagtgattg aaagaaaacc tgtgcgaatg ttagtattct catttattgt agtagctatt
    25861 ggtggcatca tcgagttcgt acctactttc ctagtcaaat cgaatgttcc gactatagcg
    25921 agtgttaaac cttatacacc gctcgaatta cacggccgtg atatatatgt aagagaaggg
    25981 tgttatactt gccattcgca acaggttaga cctttcagag atgaagtagc acgctatggt
    26041 gaatactcta aggctggcga attcgtatat gaccacccat tccagtgggg aagtaaacgt
    26101 actggtccgg atctggcacg tgagggcggt aaatacccac atagttggca ttacaaccac
    26161 atgctcgacc ctcaaagcat gtcgccgggt tctattatgc ctgcttaccc ttggttgttt
    26221 aaggataaaa tagacaccca aaagacaaaa tccatgattt gggctatgca aaaacttggt
    26281 gttccttacg aaaaaggata cggaaaagta gctaacaatg atctgaaaaa gcaaagtgaa
    26341 gaaatagtaa aatcattgaa atcggataaa atagaagcaa agccaaatac tgaaattatt
    26401 gctctgatag catacctaca gcgtttgggt aaagacatca aggctgaagg caatagtgcc
    26461 aataaataat ttattaaaat agaaaaagat gaaatttgta cattacctgg aaagaatagt
    26521 tggtgtagat atctaccctt tgctttctct tgtaattttc ggttcgtttt ttcttgtagt
    26581 ggcagtatgg cttttcagta caaaaaaatc agattttgat gaagtaagcc gtattccgct
    26641 aaatgaaaat gaacctgagc attaaatttt aataacagta gaaagttaaa acgtatggaa
    26701 aacttaatcc ttaaacagtt tataaacagc agctttttga acggtggatt cttgcttcag
    26761 gtcaatccga ctgctacaga acaatctcca tggatgtcag tgctgaccaa cccgattgct
    26821 attatcatga tactaatcat gtttgcactg ttgttcgtta tcagagcttt gacaaaagta
    26881 ctcctcgaag tagccggcaa taaaatgaaa cgcaatctgc aagacaacaa agaaggtatt
    26941 ttaaaagcga tagcattact gattgtgatg ataactgcat cttcgactgg atttgcgcag
    27001 gatgctgctg ccgctgcagt aacgacagct ccaccgaaaa taataggtgg aatggctcag
    27061 tctgtatttt atgttataac agctgttatc atcatcgaag caataataat atatgtattg
    27121 ctgatgagta tcagaaaaat ggtaactaag gataatcttg atcaatacaa tgcagggcct
    27181 gaatcgcagg gggtaatagc taaatggtgg gaccaactca atggctacaa agctgcagaa
    27241 caggcagata ttgacttggg ccatgattat gacggcattc gcgagctcga taacagattg
    27301 ccaccatggt ggttatatgg attttatgct accattttat tcgcaggtta ttacctatgg
    27361 acacaccatg taagtcactc agctcctgac tctatccagg aatataagac agaaatggcc
    27421 gttgcagaaa aacaggtggc tgaatatctg aaaaccaaag gcgacgactt caatgaaaat
    27481 accgtaaaaa tgctaggagc agaagatatt gccgaaggta aaaaaatgtt tgaaaccaca
    27541 tgtgctgctt gtcacctcgc tactggggca gggagtgtag ggcctaacct tacagatgca
    27601 tattggctta atggcggaga tatcaaaagc gtatttaaga ctattaaata cggtatcaga
    27661 gctatgccgc cttggcagaa cagcttctca ggtaagcaaa tggcacagtt ggcgagtttt
    27721 gttgaatcat taaagaatac gaatgttgcc ggtggtaaag ctccggaagg tgaagagttt
    27781 aaggaagggg ccggcgaggc acaaccacct gcagactcta cagcagctgc ggcagcacaa
    27841 aaataaaaat tgttttttag gctttagcct gtatattaga ttaaattgaa atgagccagg
    27901 acgataataa gttcttgcct gatgaatctt ttagagaccg gatggtcaca atagatgagg
    27961 atggcaacag aaaatggatt tatgctacaa aacccaaagg taaattctat aattaccgga
    28021 cctacgtaag ctatctgttc tttgctattc ttgttgtact cccatttatc aaagtgagag
    28081 gcaatccgtt gtttcttctg gatgttctca ataccagatt tattattttt ggaaaaattt
    28141 tctggcctca ggatttcttc atttttggcc ttgggatgat tgcttttata gttataatca
    28201 tcctgtttac ggcagcattt ggtcgtttgt tttgtggttg gatatgtcct cagactattt
    28261 ttatggagat gctcttccgc aaaatagaat atgcaataga aggcgacgca ccaaagcaaa
    28321 aaagactcaa tgacggtccc tggaccaatg aaaagatttt aaagaaaacg gcaaaacatg
    28381 taatattctt tgcgttatct tttttcattt ccaacttgtt ccttgcgtat attattgggg
    28441 ttgatgagtt aaagttaatc atttttgact caatatccaa ccacattggc gggtttacag
    28501 ccatactggt attttcattt atcttttatg gtgtattcgc ttggtttcgc gagcaggtat
    28561 gtacggttgt ctgcccgtat ggacggctac agggcgtatt gcttgatcaa aactcaatgg
    28621 ttgtcgcgta tgattatcag agaggtgaac cacgaggtaa ggtaaaggac aaagatatac
    28681 ttaacttggg tgactgtatc gactgtaacc tatgtgtaaa agtttgccct acaggtatcg
    28741 atatccggaa tggtacacag atggaatgtg tcggttgtac agcttgtatc gatgcctgcg
    28801 atgaaataat ggaaaaagta aacaggccac tcggtctcat ccgttatgct tctgagaacg
    28861 gcatcctcaa taacgaacca ctgcgatata cgccaaggat gaagctctat actttgcttt
    28921 tgattgctct ggtcgctttg cttagtatcc tgttgataac ccgaaaagac gtagatgcta
    28981 ccatcattcg ggctcagggt atgttgtacc aggaagtagg tactgatagc ctttcgaacc
    29041 tttataccat caagatggtg aacaaaacga ttaaaaacct taatgtttcg ctcaagttgg
    29101 agggcatacc aggtcagata aagatggcag gtgacaagga cataccagtg cataaggaag
    29161 gccagggaag tggaagcttc tttattatct tacctaaaaa tcaagtaaag gacagaaaga
    29221 ctgaagtaaa tataggcatc tacagcaatg gagaaagaat tgatgtactc tcaaccaact
    29281 ttatggggcc tatacatgtt aactaataaa taccatttat gaactggggc agaagcatca
    29341 ttattgcatt tatcttgttt gcatcgttta ttttctatat agtatattcc tcaatgaata
    29401 cacactttga ccttgtgtct gaaaattatt ataaagaaga actcgaatac caaaagatca
    29461 ttgacagcaa gcaaaatgct aataatctga aggaaaaaat tgccataaag gttgaaaaca
    29521 acaatatcaa gcttatttta cctccagaac tgcgttcaaa tactgccagt ggcaagatac
    29581 atttttacaa cgtcaccgat gcccgaactg atcagcaaat tgacctggta gtaaacgtac
    29641 aaggtgagca ggattttgaa cggaacaaat tcgtcccagg caattatgta gccaagataa
    29701 gtattaccag ctcaggtacc gattattata ctgagattcc ggtaaagata tagtggaatg
    29761 attcaagtaa ttgctcccgg tattcttttt gggttgatga gcaattttca ttgtataggt
    29821 atgtgtggcc cgtttgcact ttcattacct atccagaaca aatctactct ttataagtgg
    29881 ctgtcaatac ttgcttacca gtcaggtcgg attactacct attcaacatt aggagccata
    29941 tttggttatt ttggtaggac cattacgatc tctggtctgc agcagagcat atcaatcggt
    30001 gccggtattc ttattttact taccattatt ttgccaaggt tgggtacaca gttacaactg
    30061 atgttacctg tcaacaagat acttactcct ttactagggt atgttggtta ttatttcaaa
    30121 aatcagtctg ttggcggatt ttttatcatt ggcaatatca atggactttt cccttgcgga
    30181 atggtttatc tggctatagc cgcagcattt accacaggcg attttgtatc gagtaccctt
    30241 tttatggcag gcttcggtat cggtacgcta cctgcgatgt taacagtaag cattatgggc
    30301 caatggatga gtcagcgact gaggcataaa tttaataaat taacacccta tttttttgca
    30361 attgtaggtg tcattttgat attgaggggc cttggtctga gtattcctta tgtgagcccg
    30421 gcgcttgatg tgccgcccat aggtgtgcga gatgctgagt gtatgtagca aaagaggata
    30481 ctttttgtga taaaaaatta tctacaaaaa ttgatataca gactattatt aaaaatgtgc
    30541 gcaaaatttt tactgatttt caataactta caaaaagatt tttgaaaaaa tattgcaaaa
    30601 tagttgtaat taaaattttc tgaatgtacc tttgcatccg ctttgcaaaa agataataga
    30661 gataatcatt taaaataata gaaaaaagtg aatacattaa gttacagaac acgttcagta
    30721 aagccgcagg atgcagacat caagtggtac gttattgatg ctgaaggaga ggtagttggg
    30781 cgactatgtt caagaattgc ttctgtgtta agaggcaaaa acaaaccttc cttcactccg
    30841 cacatggata ccggagatta tgttattgta gtaaatgccg acaaggtacg gtttacgggc
    30901 aataagatgc agcagaaaga atacattagt tattccttgt atcctggggg ccagaaaaga
    30961 aaaaaagcta cccaattaat ggctaccaag ccgcttgcca tagttgaaaa aggcgttaaa
    31021 ggtatgcttc cacataacaa gttgggagat gctatattta aaaaattatt tgtttacgca
    31081 ggccctgagc atccgcacgc tgctcaaaag cccgaacctt ttaaatttta ataatcatgg
    31141 aaatgatcaa tgcagtagga agacgtaaag cttctgtagc aagagtttac ctttcaaaag
    31201 gtgaaggtaa agttaccatc aatggtaagg aagtaaaaga atattttcca caactacata
    31261 tacagcatgc tgtaacagac ccattagctg cagtagaagt agccggaatc tatgatgtaa
    31321 aagttaatgt acaaggtggc ggatttaaag gccaggccga agcagtaaga atgggtatat
    31381 caagatgtct cgtagccatc aatgaggaat tcagaaaacc attgaaagaa cgcaagttct
    31441 tgtcaagaga cagccgagag gtagagcgca agaagttcgg taagcctaaa gcaagaaaga
    31501 gcttccagtt ctctaaacgt taatttctat tttttaatac aagataaaga catatatcaa
    31561 tatgaatatt ccatctaatc aggaaatgct cgactcaggc gttcacttcg gacacctaag
    31621 aaagaaatgg aacccaaaaa tggcgcctta catcttcatg gagaagaaag gtatccatat
    31681 tattgaccta aacagaacaa gtgactgtct tgagaaagct gctcatgctg ccagacaaat
    31741 agctaaaagt ggcagaaaaa tcatgttcgt ggctaccaaa aagcaggcta gagacatcgt
    31801 taccaaagct gctcaaagcg taaatatgcc ttacgttaca gaaagatggc tgggtggcat
    31861 gatgactaac tttgctacca ttagaaagtc ggtaaagaaa atgcagaata tagaaagaat
    31921 gctcaacgac ggctctgcta ctaacctaac caagaaggaa agactcacgc ttaccagaga
    31981 aaagaataaa atggaaaagg tacttggcgg tatcgttaac cttaacagac ttccggctgc
    32041 tgtgttcatc gttgatatca tgcacgaaca tattgccctg acagaagcaa aaagactcgg
    32101 gttcaagacc ttgggtatgg tagataccaa ctcagaccct aacatggttg acttccctat
    32161 acctgcaaat gatgacgcat caaaatctat caacctgata acttctacca ttacgcaagc
    32221 catcaaggaa ggcctcgaag agagaaaaga ctcaaagatg gaagcaagag aaggtgaaga
    32281 tgctgaagca taataacata ttttaataaa caaaattata attcaaaaca atatgagcga
    32341 attagtaata tcggcatcag atgttaaaaa tctacgagac caaactggtg caggaatgat
    32401 ggattgcaaa aaagcactta tagaagcaga aggcgacttc gaaaaagcag tagaagtact
    32461 tcgcaagcaa ggtcagaaac tttctgtaaa gagagccgac cgcgaagcaa aagaaggtgc
    32521 tgtcattgca ttggtgtcac acaataagaa taaaggaata gtagtaagac taagctgcga
    32581 aacagatttc gttgctaaga atgatggatt tgtagagctc acaaagcaaa tagcagagat
    32641 tgcccttaag gaaacacctg ctactattga tgatctgttg aattgctctt ttgatggcaa
    32701 aatcagcatc gcagaaaaaa tcaatgaata ccttgcagcc atcggtgaga aaatcgaact
    32761 cgtatcttac gaatctatca gtaccaatgc aggcgaagga caggtattgt cttatgtaca
    32821 cgcaggtaac agagcaggtg tcatcgtagc acttaacctc gaaggaccac agtacgaaga
    32881 agccggaaga aacgttgcca tgcaagtagc agcaatgaaa cctctggctc ttgataaaga
    32941 tggtgttgat gcaagtattg tagaaaagga aatcgaaatc ggtaaagaac aggcacgtgc
    33001 ggaaggaaaa ccggaagcaa tgctagaaaa aatagcactt ggcaaactga acaaatttta
    33061 tcaggagaat acccttaatt cacaaactta tgtgaaggat ggtaaaatgt cagtagcaga
    33121 ataccttcaa tcgttacata aagatctcaa agtgacagcg tttaaacatg ttgctttagg
    33181 ttaattaaaa aaagcgaatc aattttgatt cgcttttttt ttaatatttc actagctata
    33241 gcttattttt gccaccaata aacaatgtca tgcctgtaaa atacaaaaga gtccttttga
    33301 agttaagcgg agaatccctg atgggtgaaa aaaatttcgg tattgaccaa tcaatgctgg
    33361 tacattatgc agaacagatt aaggaacttg tgcaggcagg tgtacaagta gctgtggtaa
    33421 tcggtggcgg caatatattc cgaggcctcg atgctgaaaa atccggaata gaacgcgtaa
    33481 caggagatta tatgggaatg cttgccacag ttatcaatgg catggccata caaagctcac
    33541 ttgaggagct gggtatctat agtcgcctga tttcggccat agaaatgaaa gaaattgcgg
    33601 aaccatacat cagacgacgc gccatcaggc atcttgagaa aaacagagta gtaatttttg
    33661 caggcggtac cggaagtcct tattttacaa ccgactctgc tgcagcactc agagccaacg
    33721 aaatagatgc agatgttata ctgaaaggca caagggtaga tggtgtttat acggccgacc
    33781 cggagaaaga tccacaagca gtaaaatttg ataaaattac ttttacaaat gctatcaacc
    33841 tcgggcttaa agtgatggat atgactgcct ttacactttg taaggaaaat aacttgccta
    33901 taatcgtatt cgatataaac aaaccggata atctgatgcg tatcatcaat ggcgaatcta
    33961 tcggtacact ggtggaagga taattgtttg taaaccgtag gtgacaaaaa tcataattgt
    34021 gtaaaatcta agtcataagg aaagaaccgg attatcagca tttttgcctg aatcaaaaat
    34081 ccgcaactat gcctttatac agacaggtgg gaaccattcc acccaagaga cacaccgtat
    34141 tccgacaaaa taacggacaa ctttatcatg aagaacttgt agggacagaa ggtttttcag
    34201 gtatttcttc acttgtttat cacttatacc cacctacttt ggtcaaagct aaaggcgaac
    34261 cctattctgt taagcctgaa atagctgtcg aagataacct gcaggcaagg agttttgtag
    34321 gttttcagct tcaacctgag catgactatt tgactagtcg taaagtacta ttcgtaaatg
    34381 acgatatgca catagggctg gcagcaccta ctaaaggact tacagacgaa tattttaaaa
    34441 atgctgatgc tgacgaattg ttatttatcc acgaaggttc gggagtattg aagacaatgt
    34501 atggcagttt gcggtttgaa tacggtgatt atttagtaat accacgaggt actgtttatc
    34561 aaatattgtt tgactccgaa acgaacaggc ttttgtttat tgagtctttt agcccgatag
    34621 aaaccccgac aaggtacaga aatcagtacg gacagtttgt tgagaattcg ccattttgtg
    34681 agcgagacct caaattgcct accgatcttg aaacgcatga tgaagttggt gactttcata
    34741 taaaaattaa gaaaagaggc cttatttatc catatacgta tgagactcac ccatttgacg
    34801 tggtaggtta tgacggttgt tgctacccgt atgctatgtc aatattcaac tttgagccaa
    34861 taaccggtag aattcacatg ccgccacctg ttcatcagca ttttcaggga aacaattttg
    34921 tggtctgttc tttcctaccg agaatgtacg attatcatcc ggattctata cctacatctt
    34981 accaccatag taacatagac agtgatgagt tgctctacta tgttgacggt gattttatga
    35041 gtagaaacaa tatttcgaag gggcagataa cgctacatcc acagggattg ccacacgggc
    35101 cgcacccggg tgccatagag cgaagtattg gcaagaagca gacgaatgaa gtagcggtta
    35161 tgatagatcc gttcagaccg gtgaagataa cgaccgaagc cagaaaactc gaacttgagg
    35221 actattacca gtcttggata gttaagccac tcgagagtaa aatgacatct gtttaaccaa
    35281 tcattcaaaa tcattatgga caatcttaca actttaaaaa actattccta ctcaggagaa
    35341 aaaatttttg aaaaggcaca ggactttttg cctatcaatg gtaccgatta tgttgaattt
    35401 tatgtaggca atgccaagca gtctgctcat tattacaaaa ctgcattcgg atttcaggag
    35461 cttgcatatt gtggacttga gaccggtgta cgcgaccgta catcgtatgt attgcagcag
    35521 ggcaaaatac ttataatact caccacacca cttgtagagg aaagcgatgt tgccagacac
    35581 atagccaaac acggagatgg tattagggta attgcactat gggtggatga tgcctatgat
    35641 gcgtttgaac aaactgtgct acgtggtgcc aaaccatttc tacagcccga aacccttgaa
    35701 gatgaaaatg gcacagtcag gcgttcgggc attcacacat acggtgatac tgtacatatt
    35761 tttattgaaa ggaaagatta taatggcatt tttctacctg gcttcgagaa gtgggaaaca
    35821 gagtacagac cttctccaat ggggcttaaa tatatagacc acatggttgg gaatgtagag
    35881 cttggtaaaa tgaatgaatg gtcagacttt tatcagaatg ttatgggttt tgccaacctt
    35941 attacattcg atgataaaga tatttcgact gaatataccg cccttatgag taaggtcatg
    36001 acaaacggta atgggtatat taagtttccg ttaaatgaac ctgccaaggg aaagaaaaaa
    36061 tcacaaattg aggaatttct tgatttttac aatggccctg gctgtcaaca tctcgccgta
    36121 gcaaccgatg acatagtgta cactgtatca gaaatgcgca aaaagggtgt cgaattcctt
    36181 tatgtacctg gtacatatta tgacagcgtc aaagaaagag tcggtatcat tgaagaagac
    36241 ctggaaatat tgaagaagtg gggtatcatg gtggatagag atgaagatgg ttatttgttg
    36301 caaatattca caaagccagt tgaagacaga ccaaccttat tttttgaaat tatacagaga
    36361 aaaggagcaa aatcatttgg taaaggtaat ttcaaagccc tattcgagtc tatcgaagca
    36421 gagcaggccc gaagaggaac actctaattt atttttcaaa aaaaacacag tgcaattatt
    36481 tttaaaggca gggaagaata tttttccctg ctttcttttt atctaatacc ttatgtagat
    36541 tccctgatat ttttactaag ccttaccgac attcttatac agatttacca agttaatatt
    36601 cataaaatat ccggcaaaat catacaccgg tatattggca attttttaaa atatattgct
    36661 gaaatatata aaaattgatg atcgcaatat attgacaatg aatgaggtat ataatatagc
    36721 tgaaaatatt cagaaatttt tattgaataa tcctgaaatc agagaaaatt ggtatcattt
    36781 tttcttataa tatgttgttc ttgcgaagtt aataactcga tataaaatcc ttagagtaag
    36841 acctgatcgc tggggggttg atgaggttct tactcttttt tagtttgttc ctattttgag
    36901 tctttccggt ctgttggcta tttcccaagc agtataaaac acgagttgtg cccgttttcg
    36961 caatagatta aaatgtattt tgtctatcgt atcgcttggt ttgtgataat cttcatgcac
    37021 accactgaag taaaaaatga ttggaatgcc tttttcagca aaattgtaat ggtctgacct
    37081 ataataaaaa cgatttgggt ctgtaggcga attataggtg tagtcaagcg tcaggttaga
    37141 gaatcttgca ttcacacttt cactcaactc atgcaattcc atactgattc ggtcagagcc
    37201 aatgatgtaa atatagttcg aatcagcact ttgaatgtgt tttgggtcta ttctgccaat
    37261 catgtcaatg tttaggtctg ctactgtgtt ctctaatgga agcaccggaa atgtagtata
    37321 atatttcgaa ccataaaggc ctttttcttc gccggttact agcataaaca ggatacttct
    37381 tttgggtctg aatccatatt ctgccgcttt ggtgaaagcc ctggccattg aaagcactgc
    37441 ggtagtaccc gagccatcat catctgcacc atgataaatg tagtcacctc ttttacctag
    37501 gtgatccatg tgtgcggtga tgatgaggac ttcatctgcc aacttagggt cagagccccg
    37561 catataaccc aagatattgt ttgatttgac atctttaaag cttagttttt ggtctatacg
    37621 gtattctccc ttaaaactca tagacaaagg ttttgctttc ttaatggctt tgtttctttg
    37681 tttaatgaaa tttgccgact ggctccctaa gatatccttg agtatatctg ttgaaatata
    37741 tgcgtgaccg atgttagaat ccactgtatt gcctacagca gtagggacca tctcattccc
    37801 cagtatggca ccgcggtatg tttcgaacat tttctgaata cctgccttta caatcaaaac
    37861 ataggctgca cctttttctt ttgcagtttt aagctttaag gcattgtctt gagcactcca
    37921 ttcggaggga gttttagagc cggtaatgac gtatttgtct ttgtgcatgg gttctccttc
    37981 atatatcagt acaacctttc ctgagacatc tacattttta taatctgaat attttgaatc
    38041 atcgattcca tagcctatat agattatttc attatagttc agttttaagt ctttattttt
    38101 cgaaggtatg gcaatgtaat caaataaatg tctgaagtca ccgccattga actcaagcct
    38161 gatgtggtcc catttgtagc tggtcatata aatgccttgc atataactct ggttgtcacc
    38221 tatggcatcc agtttcaggt tggcaaactg acgagcaata taccttgcag ccttatcatt
    38281 accgggtgta ccggtttcac gaccttcaag cgaatcggcg gtcagaactc tgaggtgttg
    38341 tttcagaatt tcttctgtta tgaaattggc gtatatctga ggttcggatt tt
//
LOCUS       DAOQQY010000003        37157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_318_length_37157_cov_7.697429, whole genome shotgun sequence.
ACCESSION   DAOQQY010000003 DAOQQY010000000
VERSION     DAOQQY010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 37157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37157
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_318_length_37157_cov_7.697429"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..5666
                     /locus_tag="PLE23_00365"
     CDS             27..5666
                     /locus_tag="PLE23_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX81745.1"
                     /translation="MAKNCKDIVLNAPPLNYTNNKTGNCAITGSVQAVIVSNYTECGG
                     TVEYLWEYTDACFRTISHTQTTVVEPAPAATFAQPPPDVTVQCTAVPNLDVPLNYSNK
                     DLGVCAISGNVYPTVIGAHDFCGGDYTQVWEFTDNCGRTITAQRKLVVLPAPLPTFTN
                     PPANITITCDQAEQIDQQMLLSYNNNLTGDCQNYGDIEAITTGSYDPCGGNLQRTWNL
                     NTCGTLITHTQNIKILPAPDATFFDPPADITLDCGEPFPPADDLYYTNNKFDQCDISG
                     FVSADQYLDNGVMTYTWEFVNPCSNKKITKVQKIYGKLVPDIFLDPAQADICLGSSFN
                     LASIQVTDNNNSNPSISYHSGTPATAANKLSSTVVSPSVTTTYYVLATNTICTDEAPF
                     TITVNTPLKAGGDGQGTVCFGANNVNLFSYLVGMYDAGGVWKNKGSGNINLFDPTKVS
                     FNNAVSGTYGFYYIVPKVGACPADTATVNITVQPDITINIESVSCTPDLDFYNVVITA
                     SGYTITASSGTVTNIGSNQYNIGPVSVSNNLTITATEVSTGCTKDFQINAPNCNCPTV
                     LPPVSGGNKKICFGEAIPTLTVTVGTDETADWYDSPTGGTLLAAGTLQYTPSISAPGL
                     YKFYVEAKSTLFPDCKSNVRTLITIEIIANPTGNNASLYACDEDSQGHAQFTLTDAIA
                     QINPNPSLTYAFYATPADANTETNALPINYTNTSTPTQTIYVVLKNQDGCKTLVTLTL
                     NIYPKIVLTLTPKPETCAGSKDATITVSKTGGSGVVEYSIDNITWTGNNVFTGLSQGS
                     YTIYGRDDKACVFSNTVVIPEGLILNIINFNIVCNNAGTDTDASDDYYVVTFNIGNNK
                     ANVGTYTLKDGATVLGTFSYGVSKSVNIPAKSQSLTLTFEDVAAGCSVTQNIGPLTPC
                     STNCEITVTTFTKVCNDAGTNTDPVDDYYTITINATAANGSASNTFKVIVGGNVVATF
                     TYGVGGTITLLADGSNPTITLVDSDDNQCQNIQNPGNLTPCSNTCVINAVIENVNCDN
                     KGTTNDSSDDTYTFDIIVNGVNTSTGWYVSGTPANVYSYTASTMFGPYLISGGSKTFE
                     LVDKDDATCKVLINIPAPPPCSDPCIIEYTNLVVGTCNDNNTGNTGTDDTFDVSFMVN
                     SVNGTVTQYNVSWNGNNWGPFDYGQTVTIPGLPANGSTIILKVTDPINQSCVTSMSVS
                     QLPCSSCPEKVDAGPSFTVDCIIKSVILQANATAVATYSWTGPNNFTSTQLNPTVSTP
                     GTYYITADFGKACLYTDSTVVILDSSVPVADAGEDKLLTCLVTEVLLDGSKSSQKPGF
                     VFEWRDENGNLLGNGLTLLVNQPGNYYFTVIDPSNNCASPPSLAVVSQNINDPVAPIF
                     ADPGEVLNCNVDAIVLSTTPQTNVVFTWTQNNSTQNGSSFTTTKGGLVTLVAVDTVSK
                     CSESNTLLIQELIEYPIVNVAQPAKITCKNRIVIIDASGSQHGPNITYQWLDSNKNPI
                     LNQTGDKLSVDKPGKYFISVEDVNIGCVNSDSVVVESDIVLPIVNAGENHNLPCFVFE
                     TKLDGTASSASNNFNIYWTTQTGKILNGDKTYTPLVNGEGWYVLHVTDLNTGCVNSDS
                     VFVKINADAPVISGVVVDSVKCYGEKNGSISITQTSGGVPPIEFYINGSKKPNTTGIF
                     SPLYAGAYTIVAKDAIGCESSTTFVVGEGSILNLSLDPSIIVRLGDSTWLEAVVNVPV
                     DELSKVLWTPAKDLSCDSCLITQVKTLSTQQYRITVQDIHGCEATDYIIVLIDKDVNI
                     FIPNIFSPNGDNINDRFTLFADDRVVKIHELKIFDRWGELMYQNYDFAPNDPNLGWDG
                     TFRGKTVNPAVFVYYFVVEYIDGSKQLYKGDITLVRE"
     gene            5730..6398
                     /locus_tag="PLE23_00370"
     CDS             5730..6398
                     /locus_tag="PLE23_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HMX81746.1"
                     /translation="MVQNFTTGDGRNESVYYLKAVDSDKTLLVFHEWWGLNTHIKELA
                     HQFANDLPGVNILAIDLYHGQVTDKDEHAAILKRSISLETANETIGAAISTFANGHLL
                     ASIGYCMGGTWALEAALLTGTQMKACVIYYGMPEMNVEILKSLQAPVLGIFGEFDPWI
                     SSAIVNRFAEAMNTAGKDLQYFIFPAQHAFFNPSNATHDPDSARKAYALTLEFLKKKL
                     FLEN"
     gene            complement(6395..9220)
                     /gene="uvrA"
                     /locus_tag="PLE23_00375"
     CDS             complement(6395..9220)
                     /gene="uvrA"
                     /locus_tag="PLE23_00375"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016099277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMX81747.1"
                     /translation="MVVEPEYIEITGAREHNLQNIDLRIPRNKLVVITGISGSGKSSL
                     AFDTIYAEGQRRYMDTFSSYARQFIGSMEKPDVEYISGLSPVISIEQKTTSKNPRSTV
                     GTVTEILDFLRLLFARIGEAHSYVTGKKMVRYSEVQMIDHVYEHFTDQKITILAPLIR
                     GRKGHYRELFDQIRKQGFLKVRVNGVMQEIVPNMQLERFKTHDIEVVIDRIQVLEKSK
                     DRLRTSMQTALKSGSGMVMVLDEKEQIFPYSKYLMDPETGISYEEPSPNTFSFNSPYG
                     ACTSCIGLGTLNQIDIDRVIPDDSKSINEIGIAPLGEVRQNNIFEQLRQYAKLKGFSF
                     STPVKDIPEDALRHILYGGDKDYKPSAALSKNEFSSFNLPHEGLLNMLQRWYRDSTSD
                     NIRAWAEEYMTIVQCSDCNGTRLRKESLSFKVADRNIGELCNMDVRSLHDWIGKLPEI
                     LNNKHRQIAAEIIKEIGLRLGFLLNVGLEYLSLNRPSRTLSGGESQRIRLATQIGSQL
                     TGITYILDEPSIGLHQRDNMKLIQALRELTDIGNSILVVEHDKDIMMCADYIIDLGPG
                     AGKHGGMLVGEGTPEKFLSLRTLTSEYLSNSKSIDVPKKRRKGNGHFLELAGASGNNL
                     KNVNIKIPLGTFVCVTGVSGSGKSTLINDTLYPILRKYFYKSKQEPLAYKKITGLENL
                     DKVIEIDQAPIGRTPRSNPVTYTNVFGDIRKIFAELPESKIRGYKPGRFSFNVKGGRC
                     EECQGGGMKTIEMNFLPDVQVECDVCMGRRYNRETLEVLYKGKSINDVLNMTVEEAVV
                     FFDSYPSIHRKIKTLQDVGLSYISLGQAATTLSGGEAQRVKLSEELSKKDTGKTIYIL
                     DEPTTGLHFEDIKLLLGVLNKLVDKGNTVLIIEHNMDVIKTVDYIIDIGPEGGDKGGS
                     IVCTGTPEQVAANKSSYTGEFLKKELT"
     gene            complement(9226..10044)
                     /locus_tag="PLE23_00380"
     CDS             complement(9226..10044)
                     /locus_tag="PLE23_00380"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HMX81748.1"
                     /translation="MFDQIKEAADYIVTHLNVKPKIGMVLGTGLSNIASEIEDEVIIR
                     YAAIPWFSHSTVASHKGEMVFGRLGEKDVVILAGRFHYYEGYTMQQVTFPVRVLKELG
                     VETILISNAAGSVNAAYEAGDIVFLKDHINLMPENPLRGPNDERLGLRFPDMSDAYDK
                     NLIEKAVEIANKNNIRCHQGVYVALQGPNLETPSEYNFINVIGGDLVGMSTVPEVLVA
                     RHCGMRVFGVSVVSNKCFPLSAIKETTHESVIAVANETEPKLAKIFKEIIQFID"
     gene            10272..11603
                     /locus_tag="PLE23_00385"
     CDS             10272..11603
                     /locus_tag="PLE23_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HMX81749.1"
                     /translation="MVKYFLSVLFILSFYELRADFFRILDDETEALQCRIDMIEQAKS
                     EILLSYYILNDDLVGMTIFSALVEAARDRKVKVCLIVDAVNSKIRKPVMRYLQDNGIE
                     VRVFKPGNILQPISYAKRMHSKLFLTDSENLIVGGRNIKREYYSLGKDFNFLDRDVFV
                     KSRSATETARKHFYSIWDNEKIAKHKDLKPISVDEKLEIENKLRLARIKIQDLKNIKF
                     ATSTDWTLNQKQTDNPPVFEHDNYHIYKGGKKIELDYKDTDEGSTKSLLKIINQAHKS
                     ILMENAYVIPTLKWRKALKKAIDRGVKVRILTNSAQTSDLMIAQAAYRNARKKLLKMG
                     IEIWEYQGPKTLHTKSTVIDDSISIVSSYNLVAFSERWSTEVAVWVADTTIARYHTRI
                     MEQNLQHALRIDKNNNQVKHRMPDDTKKLTCRKRTSLFINRYSIAWTMTLL"
     gene            11673..12230
                     /locus_tag="PLE23_00390"
     CDS             11673..12230
                     /locus_tag="PLE23_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMX81750.1"
                     /translation="MAEVKFSEDHEILRLLRNQDTSDKGFRLLMGTYKERLYYHIRQM
                     VGNHEDADDVLQNVFIKVYRNVQNFQEKSSLFTWLYRIATNEALNFLNSNKTKQTVAL
                     DEVAFNITNNGSEEFQDENEIQMKLLQAVNILPDKQKQVFNLRYNEEMSYEEISQILE
                     TSVGALKASYHHAVKKIESYIKGTI"
     gene            12234..12683
                     /locus_tag="PLE23_00395"
     CDS             12234..12683
                     /locus_tag="PLE23_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81751.1"
                     /translation="MKKEIKDELKEISSFLSEIQKPPVPKVPEQYFEGFEARMMRRIE
                     SEKSPSEQRSKESWFSTIFTYFTKPAIALSFGVLALLLIMTKYISTDTATRDIYADEL
                     AQVDKQELLSYIDSHIDQYDTEILLDDNTDLIENFDTPYPDDIPDTL"
     gene            12731..13198
                     /locus_tag="PLE23_00400"
     CDS             12731..13198
                     /locus_tag="PLE23_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81752.1"
                     /translation="MKNLLIVAMLFFSTQYILAQDNNPPQQGKRGEKIESLRIAFITD
                     KLQLTPEESKQFWPVYTQYTKDLEQLRSEAKRAKAEVGMSDAEAENMVKDRFSRQERR
                     LELDRKYYQEFRKVLPATKVALFFDSINQFNQKVLKAYKEKKREGNRPPRRRF"
     gene            13257..14156
                     /locus_tag="PLE23_00405"
     CDS             13257..14156
                     /locus_tag="PLE23_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMX81753.1"
                     /translation="MERNTLAHLSLFSVAFIYAANFSIAKIVMGGGYVPPSTFILCRI
                     TSALVLFTVYHYLFVKEKIQPDDKKPMIWLAFFGVAANQLLFFMGLALTTPIHAALIM
                     TITPISVVIVESVLRESPLTFNKVFGVLIAFTGAIFIILSGSDISFDGQTMLGDFFVF
                     ANAVFYSIYLVKVKPLVRKYHPITINRVTFLYGFFMVLPFGFPNITGIQWGAFDTRIW
                     LSFLYVLIFTTFFAYYLNSWAMLIVKPSVVGVYIYLQPLLTIIIAIIIGTDKPDINII
                     LAGLLIFTGVFLTSYTKNIKKDT"
     gene            14397..15794
                     /locus_tag="PLE23_00410"
     CDS             14397..15794
                     /locus_tag="PLE23_00410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX81754.1"
                     /translation="MKRKGQRSEQTPSQLVSDYTIMLENGEIDFIKEDDWQSLIDYFV
                     INNRLSDALEVSQKAIEQHNFSIELHINHARMLLANEFYREAVLFVEEKSYLSPNDPE
                     FILVKAQGMLNLGEEDCCQELISSIKKDADNDLLTDILLTETDLYERAQEFDLMFDAL
                     QNILLIHPDNTEALERIWLCTELSGRYEDSILLHQKIIENDSYSFQAWYNLGQAYFSM
                     EQYDQAADAFEYAYLINEDFEFAYRDRGESLLLLTHYELALNCYQEVKERFVPDADLY
                     CKIGQCYAFLEQPELAIDHFSVSLQMGNTTGEVHFHLGVCLVTLEKFIGAEQSLRKAV
                     ELEPEREEYHLALADLYFQQENYELASISYQKAVDLAPENYLIWCQYAGFLLLTHGDE
                     AAMEAIDEALFYSDSAELHYLKVVCMLSAGKRQEAIINLMKLMDTYPEHADYVYEVMP
                     DLKDDAEVTAIIAGL"
     gene            complement(15813..16706)
                     /locus_tag="PLE23_00415"
     CDS             complement(15813..16706)
                     /locus_tag="PLE23_00415"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMX81755.1"
                     /translation="MNLKSIFLLLLTTASFSILAQQADFECTSTNKKAFSNIYFKNTS
                     KKAKSYSWDFGDGRSSTEVSPTHQYASAGTYTVTLTAIKGKKQNNKTMTLEISAPEHC
                     TVEIETPFGTMTAVLYDQTPHHRDNFLKLAGQGFYDSLLFHRVINGFMIQGGDPTSKG
                     ASAGVPLGSGGPGYTIPAEFNSSLAHTKGALAAARMSDAVNPDKRSSGSQFYIVQGRP
                     VDPRSLQRTEAAKGFNYPKDIVEKYERLGGTPFLDMDYTVFGEVIKGLDIIDKIAAVK
                     TLPGDRPEQDVWMKIRVVNND"
     gene            complement(16806..17165)
                     /locus_tag="PLE23_00420"
     CDS             complement(16806..17165)
                     /locus_tag="PLE23_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81756.1"
                     /translation="MEDWQLDFDWLRIQHLIKDQLGNDRLPDMNTVLILIGIQELGMP
                     RKKWTKEEKQDLMHIGVCSLLSHDGYYEFIGRDEDGWPHFKQLRKVSAEGVDNQEKLL
                     KAEAIKYFDVLAKENSW"
     gene            complement(17265..18491)
                     /locus_tag="PLE23_00425"
     CDS             complement(17265..18491)
                     /locus_tag="PLE23_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMX81757.1"
                     /translation="MNRTLSFIFSRKYFYSKRSANAIHYISTISILGISIGTAALILV
                     LSVFNGFEGLITKMLNQFNPDIKITPREGKFFERDSVMIKKLENTEGVLAVSKVLEEK
                     ALFESEKVQDFGTLKGVDKHFRQVASLDSAILEGEYMLTKDKTDYGIIGFGLANKLGT
                     MPSQLSEPISVYSPNEDEAGIISQPFTRRFLYPGALFSIQQDVDYEYVITSLEFVEEL
                     LNKPNSLSAIELKIDNKKNVKTLQKNIQAVVGEKFDVKNRYQQEEAFLKLMNIEKWVS
                     FAILSLTLLLVAFNMTCALWMLVLEKKSDLATLKSLGMKDKQVFSIFLNLGMLLSVVG
                     FLVGLAVAVILYILQKTVGIVGVPEGFAVNVYPVQMEITDIAAILIIVSMIGFLASYF
                     PAKMASRSSTLFKYGD"
     gene            complement(18493..21465)
                     /locus_tag="PLE23_00430"
     CDS             complement(18493..21465)
                     /locus_tag="PLE23_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HMX81758.1"
                     /translation="MPVNLSDKMLKKRKKILSALLLIGFLCISPKSNSIVFNKMPYNV
                     QKSDVWVDSLMNTLTLDQKICQLMMVRAHSNKTPEYEQDVANLIKNYQVGGLCFFQGT
                     PGKQANLTNYYQSLANIPLMVSMDAEWGLGMRLTDSVMSFPKSLTLGAIRDEKLINDM
                     GKEIALQLKRLGVHINFAPVADINNNPDNPVINFRSFGENRENVAVKATAYMKGLQDN
                     GIMACAKHFPGHGDTDVDSHYDLPVLTQPMARLDTLELFPFKTLISQGVQSVMTGHLN
                     VRAIENNPKVPVSLSAKAINNLLVKTMGFRGLIFTDGLEMKGVTKNYESGEVEAEALL
                     AGNDVLLLPESVPQALKTIKEYIENGKLKEEDINNKVRKVLEAKYRFGLSNYQPINLF
                     NLAYDLNSEKAIALNRTLIENALTLVRNPSDKIPLKNIEDLKLTSVAVGTKSVQTFQN
                     TIDKYVKCSYYTFGKTDGEDRKKQIINHCRNKQYVIVSLHGLTNNPKNKYNLTANEID
                     LVRDIAAVSKVILVNFGSPYILKNFDQIPYLLQAYEDTPDFQDLAAQVLLGGATCKGK
                     LPVTSSFKSRYGLGLNINKPIRIGYDLPERVGLSSIKLNAIDLLANDAINIKSTPGCQ
                     VLVAKDGKIVFDKSFGYTTYDKTTAVTARSIYDIASITKIAATTLSVMKLQQEGKISI
                     YEPLSSYLDELCYSNKEYLLIKDIMTHRAGLRSWIPFYEKTLVRVGNKSFPSPEVYSR
                     KRTSVFSIKVTDSLYMKNSYREVMYNEIVNSELPNVGHYRYSDLGFYLLAEAITRITG
                     TTIDKFADKSFYHSLALQTTGYRPINRFHKSRIVPSEMDNYFRFRKIQGYVHDMGAAM
                     LGGVSGHAGLFSNSRDLAVIMQMLINKGSYGGKEYLTPQIVRLFTSRCDGCNRRGLGF
                     DLGVTDRQTPSNMSKLASDKTFGHTGFTGTCVWADPENDIIFVFLSNRTFPTMNNRKL
                     VKEDYRERMHTAIYESLL"
     gene            complement(21465..23420)
                     /locus_tag="PLE23_00435"
     CDS             complement(21465..23420)
                     /locus_tag="PLE23_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX81759.1"
                     /translation="MAKSIANTTMGSMTELPAWMNIRTLRVIIFMLGFLLYANTLSYD
                     FTQDDAIVIYDNMYTTQGIKGIPGILEYDTFYGFFKEAGKANLVAGGRYRPFSLIVFA
                     VLYEFFGNNPFGFHLFTVMLFSITCVVLFNVLNWLFSRRFKSSQALIIAFISAVLFTG
                     HPIHTEAVANIKGVDEILALLGSLLTLCFLIKYKEAKKLKDVLAAITVFLLALLSKEN
                     AITFLAVIPLCFFVFYDEKPSTGIYRIWPLILSVAIFFMLRTKAIGLAFGNEPMELMN
                     NPFLKLSGNQYIAFTTGEKFATILFTLGLYVKLLFIPHPLTHDYYPRHIDIMSFGDIG
                     VILSGLIYLAMLVFVFKKIKEHILYAFGILYYLITISIVTNIVFPIGTNMSERFLFMP
                     SVGFCIFAGYGIHQLASRFFNKNPKSLNYNAALAITGIICLLYSFKTFTRNTVWKDNY
                     TLFITDVLTSVNSAKLQNSAGGELIAKAGKTKGTAEKTRLINEAIGHLEKAISIHPTY
                     HNAYLLLGNAYFLSEKYDEAIKQYDKCLALYPNNKDALTNLHLAYREAGKYFGEKKGD
                     LKTALEYLNKAYELNPQDYDTNRLLGVANGISGQHQKALEYFVRAAEINPVSEAFNNL
                     SHAYYNAGDKVNGDKYAALASKGQESK"
     gene            23603..24982
                     /gene="mtaB"
                     /locus_tag="PLE23_00440"
     CDS             23603..24982
                     /gene="mtaB"
                     /locus_tag="PLE23_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HMX81760.1"
                     /translation="MSTPGSVAFHTLGCKLNFAETSSISRSFKDIGFSIRNFEEQADF
                     YVINTCSVTDFADRKCRQVVRQALKRNPDAKIIVIGCYAQLKPQEIAEMEGVDLVLGA
                     AEKFNVVQYIDMLQTSTQKGLVFASEVNAANTFISSFSFGDRTRTFLKVQDGCNYKCT
                     YCTIPQARGESRSDTIDNVLKNVDDIAAKGCKEIVLTGVNLGDFGNGTEVIEGTRPQK
                     EAMFYDLVKELDKCQGIERFRISSIEPNLLTGEIIDFVAASTKFVPHFHIPLQSGSNK
                     ILRLMRRRYLRELYANKVAEIRNKIPDACIGVDVIVGFPGETNDDFLETYQFIKDLDV
                     SYLHVFTFSERENTLAAEMEGVVSVEERRRRNEMLRILSEKKKRAFYDRFVGKEKDVL
                     FEAHKDKRKLTGFTDNYLKVELEYQDNLINTIGKVKLISLNNEGDFIAERVLQQKLSV
                     ATDTISEHA"
     gene            24975..26108
                     /locus_tag="PLE23_00445"
     CDS             24975..26108
                     /locus_tag="PLE23_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81761.1"
                     /translation="MHRLFIIFILALNTYAHAQIYVTPTLLGKLSDDLKESSGLLIQS
                     ADTLWSHNDSGNSAKVFAFDKTGKKIKDRKFTKISKEDWEDICRDENGYVYVGDIGNN
                     DNNRKNLLILRFSSGFYDKDEPLVVDSLQFQYEDQTLFPPPSSSLNFDCEAFVVMNDT
                     VFLFTKDRTKPYISQTLLYKFPATPGQHTAKYVSKFMTDVPLYLQGSVTGAGLSPDGK
                     KLLLTGYLRMWLFTDFEGSNFFDGKLTVMQYNEYSQKESVAFVDECTVLMTDEYNAEL
                     NNGGNLYSLNICTTVAVDEATTSASPVVYFNHAQGILTVSRVSAACELILYSVNGKTV
                     AREKIPVGSENIQIKTLNSLAPGMYVYTIKEKSGFYSGKLMVY"
     gene            complement(26105..27178)
                     /gene="porQ"
                     /locus_tag="PLE23_00450"
     CDS             complement(26105..27178)
                     /gene="porQ"
                     /locus_tag="PLE23_00450"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HMX81762.1"
                     /translation="MKILRGNLVQCLILTFIVCRASTVSAQLGGVGVYKFLWNPPDAH
                     ITALAGNGIAIRDDDAALAMQNPSLLNENSNDKLSFNHRFFYSGLQDGTFTYAFSPKS
                     IPYHFHAGVQYANYGNFTQADEYGNITGSFKAADYALIVGASRNLYENLSLGVNLKFI
                     TSQLESYSSTGLAADIGASYFIPNKNFTIALVCRNIGTQLTDFNDKPERLPYELQAGI
                     SKRLAHLPFRFSIVYTNLQRWNVLYDDPEQVKENDIFTNQNAQRSDASIFIDNLFRHF
                     VFNGEFLIGKKENLRLRIGYNHLLRKELSVNNYRSLAGFSGGLGFKISKFRFDYGMTI
                     YHLTGKAHHLSLSTDIDSFRKGH"
     gene            complement(27159..27806)
                     /locus_tag="PLE23_00455"
     CDS             complement(27159..27806)
                     /locus_tag="PLE23_00455"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HMX81763.1"
                     /translation="MKFQYFLLIIFTAFTFGCKNIGRQPDNPTVNDTLSATEVQPDPD
                     IDSSLIHDTSRPDGVYEDKNANGIVTERGEIRNGVQDGKRYTYFADGKPRTEENFVNG
                     SYEGTFKEFHQNGKLFQEGHYSHNAMNGIWKTYYDTGELKEEVTMVANNENGPFKEYH
                     KNGKIKYEGTYKDGDHEDGEMKVYNEEGELIKKLDCKMGECNTIWVKKAYEDFKR"
     gene            complement(27874..29184)
                     /locus_tag="PLE23_00460"
     CDS             complement(27874..29184)
                     /locus_tag="PLE23_00460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HMX81764.1"
                     /translation="MNIAVVGTGYVGLVTGTCFAETGNQVICVDNNLNKLESLKEGNI
                     PIFEPGLDVLFNRNVEEGRLKFTSDLKSAVEQSSVIFLALPTPPDEDGSADLSYILGV
                     ANDLSTMIDTYKIIVDKSTVPVGTAELVHAAIAKNTSPDLFDVVSNPEFLREGLAVED
                     FMKPDRVVIGTSSDKARKYMDQLYRPFVRQGNPIFYMDERSAEITKYAANAYLATRIS
                     FMNEIANLCERVGANVDMVRMGMGSDSRIGKRFLFPGVGYGGSCFPKDVLALYHTAEQ
                     YKYDFRILKSVMAVNEKQKFVLFEKISTYFNHNLKGLKIAVWGLAFKPNTDDIREAPA
                     LYVIDALLNAGAHISVFDPEAMDNVKKIYGDKLSYTDNAYAAAKDANALAIITEWSEF
                     RNPDFERLASLMADKIIFDGRNVYELEQMAETGFHYESIGREII"
     gene            complement(29306..31417)
                     /locus_tag="PLE23_00465"
     CDS             complement(29306..31417)
                     /locus_tag="PLE23_00465"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMX81765.1"
                     /translation="MALITQIRKRSSLIIALIALGVFGFILMDIQGNRSIAGTGSTLG
                     TVAGENIDYMEFQNVEEALFKNSTAEPYARRSFLWEYLVDKVLVTKEADKLGLGVSTD
                     EMKDLAFGNNLSPVIQQQFADQQTGRIDRTQLNEVKKAIENNTLPEEGRRAWAEVERE
                     IFKEKLQEKFSNLVVKGIYTPNFMAEALQKERDDMVDLTYVKVPFETINDDQVKLEDS
                     DYAAYLKENKGAYTSDQETRRVSFVVFDVKPTAKDSADVRGKIEAIINAYKTSTEPDS
                     VFVPKNNGQMTSIYVKKAELEGMLKDTAASLPVGTIFGPYIENNAYYAFKIIDKKSLP
                     DSVKSRHILLQASTPGDIAKAQKTIDSLKVVIETGKNSFDSLALRFGQDASRDKGGDL
                     GYVAQGQMVPEFNDLIFNKAVPNKLYTVKTQFGVHLVEVTGQKFVDNKPGVKFSLFRE
                     ALVPSEETQAAAEDKANKFLAENKSVDDMKKSTAGSKEIELIKSIPLKANDYSLGVLG
                     AGEASYSIIKWAFTGEAKIGDIAPNVYSFKNAQEFYIDKYVVAGLSGIQKAGMPDVAA
                     VKDDIKTQVINRKKGEMIKAKITTKDLNAIAANFTTKVDSATGVNFAAPFVAGIGNEP
                     KVLAKAFKLGVNTTSDPIIGNSGVFVINPISKTPGAVPPNMALIKNSTSSQYASLVAP
                     KVMEALRKTYKIKDNRYKFFN"
     gene            complement(31621..32196)
                     /locus_tag="PLE23_00470"
     CDS             complement(31621..32196)
                     /locus_tag="PLE23_00470"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HMX81766.1"
                     /translation="MLIIQDKLVNDNVLQSYFACDLNACKGACCWEGDYGAPIENDEI
                     DQIDSNLTSIQQYLDQESNSIIDTEGYSTYYKDINTLGTSLKANGDCVFLKRENGVAY
                     CAIEKAYYDDKSDFLKPVSCHLYPVRIDHDPHTGFEILSYHRWHICNAACAKGKKEQI
                     PLHKFIKQGIIRKYGEEFYNELEAFAAYIKK"
     gene            32296..32712
                     /locus_tag="PLE23_00475"
     CDS             32296..32712
                     /locus_tag="PLE23_00475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81767.1"
                     /translation="MKKLFILMFICSFASGLFAQSTNSTREEAKRTTDILKAKYNLDA
                     MQEMQMQKIQERRVENAREFEYLKNTDENLYYKKKRSNIEGTNASIKRMLTSDQIKIY
                     QQDQLELRKKRAEKSKELKSKGISGFELEKALIDIE"
     gene            32822..34129
                     /locus_tag="PLE23_00480"
     CDS             32822..34129
                     /locus_tag="PLE23_00480"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HMX81768.1"
                     /translation="MKFFEKAQIASENLKKLAKDNGDLKLDEVTVSQMIGGMRGVKSL
                     LWETSELDAELGIKFRGYDIFQLRELLPKFNGGTEPLPESLFWLMLVDEIPTQAEALW
                     LTDQWVRRSLIPEHVFKALDALPTSTHPMTQFSIGISALQTESIFARRHAEGMNKNEY
                     WNATYEDTMNLLAKLPRLAAYIYRRSFHNGKHISPDITLDWAGNFAHMLGISNPEFKE
                     LMRLYLTIHGDHEGGNASAHTTHLVGSTLSDSYLSLAGGMNALAGPLHGLANQEVISW
                     IIKMMDILGTETPSDKQVEEYIKATLAGGRVVPGYGHAVLRKPDPRFLVQKQFAEEYG
                     LKSNLLNVVWQLFRLAPPMLESTGKVKNPWPNVDAHSGASLVYYGLKEHNFYTVLFGV
                     SRSLGVLASLCWSRALSLGLERPKSVSTKWLHTFVANQLATKS"
     gene            34237..34626
                     /gene="gcvH"
                     /locus_tag="PLE23_00485"
     CDS             34237..34626
                     /gene="gcvH"
                     /locus_tag="PLE23_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMX81769.1"
                     /translation="MTFPAELKYTQEHEWIRIEGDFAFIGITEFAQSELGDIVYVEVE
                     TVGEVVNSGDVFGTIEAVKTTSDLFMPITGEVLELNEAISEAGGDDPALINSDPYGEG
                     WIIKVKISDKSEIDGLLDADAYAALVN"
     gene            34781..36583
                     /locus_tag="PLE23_00490"
     CDS             34781..36583
                     /locus_tag="PLE23_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX81770.1"
                     /translation="MRYFIIFFFLLVSLGLQAQEVKLAQQYYSTGEYEKAATLYQKLL
                     SQNENNEYYFNKYIECLMALQDFESGEKAIRKQIKKFPKEVSYYVSLGKLLERQNRKE
                     EAEAQYTKAVEELPADQGSIIKLANAFVTLANYDKAIETYEKGSKLLNNKSIFSYSLA
                     DLYFRKGDTAKMIQYYLYALSLDTNLKVSLQTFFQNSFTDKEYDELQAQLLEKIQENP
                     DNLDYVEMLVWQYMQRKNYASALRYTKSIDKRLQGNGSKIYQLGSTALLQKDYDAAID
                     AFTYIITEKGKISPYYIEAKKEQLKARRLKITDTYNYTQEDLKQIEADYENFIETSGK
                     NFQTSQIMLEWATMEGLYLNNLPKAISILNEMINLQGINRNIQAEAKLQLGDFYLMTG
                     EVWEATLLYSQVDKDFGEDVLGHEARFRNAKLSYYNGDFDWAQAQFDVLKASTSKLIS
                     NDAIDLSVFILDNVGLDSITKPLAMYAQAELLVFQNKIDEAFSKLDSISTNYPEHGLL
                     DDIAYLKAKIYTKRKDYTKAIEMYSYVVEKFPEDIRADNALFEMADLYENALNDKEKA
                     KELYEKLFMNYSNSILATDARKRFRQLRGDKIFQ"
     gene            36612..36923
                     /locus_tag="PLE23_00495"
     CDS             36612..36923
                     /locus_tag="PLE23_00495"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HMX81771.1"
                     /translation="MYIYNVTLKVDPSIEEDWLIWMKDIHIPEVLSCGIFKDYKMLLL
                     TNSPFYDESPGSTFIVQYFFDNMDEYERYQAEYAPTYQREHIQRYGEKVLAFRTVMRY
                     L"
     gene            complement(36932..>37157)
                     /locus_tag="PLE23_00500"
     CDS             complement(36932..>37157)
                     /locus_tag="PLE23_00500"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX81772.1"
                     /translation="DPRFVDARRNLGCLMAINRDFPNAIKEFEKALEYDPNNADILFF
                     MGSAYRDGGNPKKGNEILEEAYRLKPELRK"
BASE COUNT        11223 a         7342 c         7230 g        11362 t
ORIGIN      
        1 cagcattcat taatccacca gctgacttgg ccaagaattg taaggatatt gtcctaaatg
       61 caccgccact taattataca aacaacaaaa caggaaattg tgccataaca ggttcggttc
      121 aagcagtaat agttagtaat tataccgaat gcggcggtac cgtagaatat ctttgggaat
      181 atacggatgc ctgcttccgt actatatctc atacgcaaac taccgttgta gaacccgcac
      241 ctgctgccac cttcgcacag ccaccacctg atgtaacagt tcagtgtaca gctgtaccaa
      301 atcttgatgt tccattgaat tattctaata aagaccttgg tgtatgtgct atatccggaa
      361 atgtttaccc tactgttatt ggcgcccatg atttctgtgg tggagattat acccaagtgt
      421 gggaatttac agataactgt ggtagaacaa ttactgcaca aagaaagctt gtagtattac
      481 ctgctccatt gcctacattt actaacccac cagctaatat taccattaca tgtgaccaag
      541 ctgagcagat tgaccagcaa atgttattgt cttataataa caacctgaca ggcgactgtc
      601 agaattacgg agatatagaa gccattacta ctggatctta tgatccatgt gggggtaatc
      661 tccagcgtac ctggaacctt aacacatgcg gaacattgat aacacatacc caaaacataa
      721 aaattttacc ggcaccagat gcaacattct tcgatccacc ggctgatatt acgcttgatt
      781 gcggtgagcc attcccgcct gccgatgatt tgtattatac caataataaa tttgaccaat
      841 gtgatataag tgggtttgta tcagcagacc aataccttga caatggcgta atgacatata
      901 catgggaatt cgttaatcca tgttcgaata aaaaaattac taaagtacaa aagatttatg
      961 gtaagctggt gccggatatt ttccttgatc ctgctcaagc tgatatttgc ctcggctcat
     1021 catttaatct ggctagtatt caggtaacgg ataacaataa ttctaatcct tcgataagct
     1081 accattcggg tacacctgct acagctgcca acaaactttc atcaactgtc gtaagtccat
     1141 cagtaaccac cacatattat gtactggcca caaatactat ctgcacggat gaggcaccat
     1201 ttactattac ggtcaacact cctctcaaag caggtggtga tggtcagggc acagtgtgtt
     1261 tcggtgctaa caatgtcaat ttgttcagtt atctggtcgg tatgtatgat gcaggtggtg
     1321 tttggaagaa taaaggctca ggcaatatca acctgtttga ccctaccaag gttagcttta
     1381 ataatgctgt ctctggtact tatggttttt actatatagt acccaaagtc ggagcttgcc
     1441 ctgcagacac agctacagta aacatcacgg tgcagcccga tattactata aatattgaat
     1501 cagtaagctg tacgccagac cttgatttct acaatgtagt aatcactgcc tcaggttata
     1561 ccatcacagc atcatcagga acagttacga atatagggtc taatcagtac aatattggtc
     1621 cggtgtcagt tagtaacaat ctgactatta cagctacaga ggtatctacc ggctgtacaa
     1681 aagattttca gatcaatgca ccaaactgta attgtccgac cgtactacct cctgtaagtg
     1741 gcggcaataa aaagatttgt tttggagaag caataccaac attgacagta acagtaggaa
     1801 cagatgaaac tgcggactgg tatgacagcc ctaccggagg cacattgctg gcagctggta
     1861 cacttcaata tacgccatcc atttcagcac cgggtttata caaattttat gtagaagcta
     1921 aaagtaccct tttcccagat tgtaagagca atgtccgtac acttattacc atcgaaatta
     1981 tagcaaaccc taccgggaat aatgcttctc tttatgcttg tgatgaagat agtcagggtc
     2041 atgctcagtt tacacttaca gatgccattg cacagataaa tccaaacccg agccttactt
     2101 atgcattcta cgcaacaccc gcagatgcta atacagaaac caacgctctt cctataaatt
     2161 ataccaacac ttctacaccg acccaaacca tttatgttgt attaaaaaat caggatggat
     2221 gtaaaacttt ggtgacgcta acgctaaata tttatccaaa aatcgtactt accctaacac
     2281 caaaacctga aacctgcgca ggctcaaagg atgctaccat tactgtgagc aaaactggcg
     2341 gatcgggtgt agtagagtat agtattgata atataacatg gacaggaaat aacgttttca
     2401 caggtttgtc gcagggcagt tatacaatct atggccgtga tgataaagct tgtgtcttca
     2461 gcaatacagt agttatacct gagggtttga tattaaatat aataaacttc aatatcgtgt
     2521 gtaataatgc cggtacggat accgatgcca gtgatgatta ttacgtcgtt acttttaata
     2581 ttggaaataa taaagcaaat gtcggtacct ataccttgaa agacggagct actgtattgg
     2641 gcacctttag ttatggtgtt tcaaagtcag tcaatatacc ggccaaatca cagtctctga
     2701 cactgacatt cgaagatgta gctgccggat gttctgttac tcagaatatt ggtccgctca
     2761 caccgtgctc taccaattgt gagattacgg tcactacatt taccaaagtg tgtaatgatg
     2821 ccggtactaa taccgatccg gttgatgatt attacacaat aaccatcaat gcaacagcag
     2881 ccaacggaag tgccagcaat acttttaaag tgatagtggg tggcaatgtt gtagcaacat
     2941 tcacctatgg tgttgggggc accattacac ttcttgctga cggcagcaat cctaccatta
     3001 cacttgtgga cagtgatgat aatcaatgcc agaatattca aaatccgggt aaccttaccc
     3061 cttgttcgaa cacctgcgtt atcaatgctg taatcgaaaa tgtgaattgt gacaacaaag
     3121 gaactaccaa tgactctagt gatgatacct atacctttga tatcatagtc aatggagtga
     3181 atacatcaac gggctggtat gttagtggca cacctgctaa tgtttactct tatactgcca
     3241 gtacgatgtt tgggccttat ctcataagcg gaggtagtaa aacattcgaa ctcgtagata
     3301 aagatgacgc cacttgtaaa gttctgatca acattccggc accaccacca tgttccgacc
     3361 catgtattat agaatatacc aatctggtag ttggtacttg caatgataat aatactggca
     3421 ataccggtac ggatgatacc ttcgatgtat cttttatggt caattctgta aatggtactg
     3481 ttacgcaata taatgtaagt tggaatggta ataactgggg tccgtttgat tatggacaga
     3541 ctgtaacaat accaggatta ccggctaatg gttcaactat aattctcaaa gttacagatc
     3601 cgatcaatca aagttgtgtc acttcaatgt cagtatcaca acttccttgc tcttcatgtc
     3661 ctgaaaaagt ggatgcggga ccgtcattta cagtggattg tataataaaa agtgtaattc
     3721 tacaggcaaa tgccacagct gttgccactt atagctggac aggacccaac aattttacat
     3781 ctacacagct aaaccctaca gtgagtacac ctggaacata ttatattact gccgattttg
     3841 gaaaagcatg cctttataca gattctaccg tagtaatact tgacagcagt gttccggtag
     3901 ctgatgctgg tgaagataaa ttgttgactt gtctggttac agaagtattg ctcgatggta
     3961 gcaaaagctc tcaaaaacct gggttcgtat ttgaatggcg tgatgaaaat ggtaatctct
     4021 tgggtaatgg actcacattg ctggtcaatc agccgggcaa ctattatttt accgtcattg
     4081 atccctctaa taattgtgct tctccaccgt ctcttgcggt agttagtcag aatatcaacg
     4141 acccggttgc acctatattt gccgatcctg gcgaagtgct caattgtaat gtggatgcaa
     4201 tagtgctttc tactacacct caaacaaatg ttgttttcac ctggacacaa aacaacagca
     4261 cacaaaatgg atcaagtttc acaacaacca agggtggcct tgtaactctc gtggccgttg
     4321 atacagtaag caaatgttca gaatcaaata ccttgctcat tcaggaactt attgaatacc
     4381 ctatagtaaa tgttgctcaa cctgcaaaaa ttacctgtaa aaatcggatt gttatcattg
     4441 atgcttccgg ctcacagcat ggtccgaata tcacatatca atggcttgat tcgaacaaaa
     4501 atcctatcct taaccagaca ggtgataaac tttctgtaga taaaccgggt aaatatttta
     4561 tttcagtaga agatgtcaac attggttgtg tcaattcgga tagtgttgta gtagaaagtg
     4621 atattgtatt gccgatagta aatgcaggcg aaaatcacaa tctgccttgt tttgtgttcg
     4681 aaaccaaact ggatggtact gcttcatctg caagcaataa ttttaacatt tactggacaa
     4741 cccaaaccgg caaaatactt aacggtgata aaacatatac tccacttgta aatggtgaag
     4801 gatggtatgt attacacgta acagatctaa atacaggatg tgttaattca gattcagttt
     4861 ttgtaaaaat taatgccgac gctccggtta tatctggtgt tgtagttgat tctgtaaaat
     4921 gctatggcga aaagaatgga agtataagca ttacccaaac atctggtggc gtaccaccta
     4981 tagaattcta tattaatgga agtaaaaaac cgaatactac cggtattttt agtccactat
     5041 atgcaggtgc ttataccatt gtagctaaag atgcaatcgg ttgcgaatca tcgaccacct
     5101 ttgtagttgg cgaaggttct atactcaatc tgtctcttga tccaagtatt atagtaaggc
     5161 tcggtgattc aacatggctt gaagcagtag taaatgtacc tgtagatgaa ctcagcaaag
     5221 tactttggac accagctaaa gatctgagtt gcgactcttg cctgattact caggtcaaaa
     5281 cactcagcac acaacagtac agaataacgg tacaggatat acatggctgt gaagctactg
     5341 actatatcat tgtgctgata gataaagatg ttaatatatt tatcccgaat attttctctc
     5401 ctaatggaga taatatcaac gacagattta cattatttgc agatgacagg gttgtaaaaa
     5461 tacatgaact caagattttt gacagatggg gagaactgat gtatcaaaat tatgattttg
     5521 caccaaatga tcctaatctg ggatgggatg gtacattcag aggaaaaaca gtcaatccgg
     5581 cggtctttgt ttattatttt gtagtagaat atattgatgg tagcaaacaa ttgtataaag
     5641 gtgatatcac cctggttagg gaataagtat cattccacca tcttggtagt gaatattttg
     5701 tatctttaat cgtctaatac tgattaaata tggtacagaa tttcactacc ggcgatggca
     5761 gaaatgaatc ggtttattac ctcaaagcag ttgactcaga taagacttta cttgtgtttc
     5821 acgaatggtg gggtcttaat acccacatta aagagcttgc tcatcaattt gccaacgact
     5881 tgcccggagt gaatattctc gccattgatt tgtaccatgg acaggtaaca gataaagatg
     5941 aacatgcagc aattctcaag cgatccattt cactagaaac agcaaatgaa acaatcggtg
     6001 ctgccataag cacattcgca aatgggcatt tgcttgcatc cattggatat tgtatgggtg
     6061 gtacttgggc gcttgaagca gccttactta ccggtactca aatgaaagcc tgtgttatct
     6121 attacggaat gcctgaaatg aatgtagaaa ttttaaaatc tcttcaggca ccggtacttg
     6181 gtatcttcgg ggagttcgat ccctggattt catcagcaat tgttaataga tttgctgagg
     6241 ccatgaatac tgccggcaaa gaccttcaat actttatttt ccctgcacaa cacgcatttt
     6301 ttaatccatc aaatgccact catgaccctg actcagcccg caaagcttat gccttaacgc
     6361 ttgaattcct aaagaaaaaa ctgttcttgg aaaattaagt aagttccttt ttcaaaaatt
     6421 cacctgtata actactctta ttggctgcta cctgttcggg agtgccggtg cacacaatgc
     6481 ttccgccttt gtctcctcct tcaggtccta tatcaatgat ataatctact gttttgataa
     6541 catccatgtt gtgttctatg atgagtacag tattgccttt gtccacgagc ttattcagta
     6601 cacccaacag taattttata tcttcgaaat gcagccctgt ggtaggttcg tctagtatat
     6661 aaatggtttt tccggtatct ttttttgaca attcttctga gagtttaact ctctgtgctt
     6721 caccacccga cagtgttgtg gcagcctgac ccagcgaaat ataactcaac ccgacatcct
     6781 gtaatgtctt tattttccgg tgaatggaag ggtagctgtc aaaaaacact actgcttctt
     6841 caaccgtcat gtttaataca tcattgatag atttgccttt gtacaatact tctagagttt
     6901 ctcggttgta cctcctaccc atacatacat cacattctac ctgcacatca ggcaagaaat
     6961 tcatctcaat ggttttcatg ccaccgccct gacattcttc acaccggcca cctttaacat
     7021 taaatgaaaa tcgccccggt ttgtaacccc tgatcttcga ctccggcaac tctgcaaaaa
     7081 tctttctgat atcgccaaat acattggtat aggtcaccgg attagaacgc ggtgttctgc
     7141 ctataggtgc ctggtctatc tctattacct tatcaagatt ttcgaggccg gtaatttttt
     7201 tgtaagccaa cggctcttgt ttgcttttgt aaaaatattt cctcagtatt gggtataggg
     7261 tatcattaat caaagtcgat ttaccactac ctgaaacacc cgtcacacaa acaaaagtcc
     7321 ctagcgggat tttgatattg acgtttttca gattattgcc acttgcacct gccagttcaa
     7381 gaaagtgccc gttgcccttc ctccttttct tcggtacatc tatagattta gagttagata
     7441 ggtattctga tgttagtgtt ctgagcgata agaatttttc gggcgttccc tctcctacaa
     7501 gcattccgcc gtgtttacct gcaccggggc caaggtctat aatatagtca gcacacatca
     7561 tgatgtcctt atcatgctct actacaagga tgctgttacc tatatcagta agctctctca
     7621 atgcctgaat gagtttcata ttatcgcgtt ggtgaagccc aatgctcggt tcatcgagga
     7681 tgtatgtaat acctgttagt tgtgacccaa tctgagtagc gagcctgatt ctctgcgatt
     7741 cgccacccga tagtgtcctt gatggacggt taagagaaag atattcaaga cctacattca
     7801 acagaaaacc caaacgaaga ccaatttctt taatgatttc tgcagctatc tgccggtgtt
     7861 tattgtttaa tatttccggc aattttccaa tccagtcgtg aagcgatctt acatccatgt
     7921 tgcatagttc gccaatattt cggtctgcga ccttaaatga caatgattcc ttccttagtc
     7981 ttgtaccatt acaatcacta cattgtacaa tggtcatgta ttcttccgcc catgcgcgaa
     8041 tattatcgga tgtagagtcg cggtaccaac gttgcagcat attcaacagc ccttcatgag
     8101 gcagattgaa gctgctaaat tcgttcttgc taagtgctgc agatggtttg tagtccttgt
     8161 ctcctccgta cagtatatgc ctgagcgcat cttcaggtat gtcttttaca ggagtagaaa
     8221 aactaaatcc ttttaatttg gcatattggc gaagctgttc aaaaatattg ttttgcctta
     8281 cttcacctaa aggtgcaata ccgatttcgt tgatactctt gctatcatcg ggtattacac
     8341 ggtcgatgtc tatctgattt agcgtaccga gcccaataca gctagtgcag gcaccgtacg
     8401 gtgaattgaa agaaaatgta ttgggcgaag gctcttcgta cgaaattcct gtctcaggat
     8461 ccatcagata ttttgaatat ggaaatatct gctctttttc atccagcacc attaccattc
     8521 cacttcccga tttgagtgca gtctgcatag atgttcggag gcggtctttg cttttttcca
     8581 acacctgtat ccggtcaatg actacttcaa tatcatgcgt cttgaacctt tcaagctgca
     8641 tattgggaac gatttcttgc atcacaccat tgacacgcac ctttaaaaaa ccctgcttgc
     8701 gtatctggtc aaataattct cgatagtgtc cttttcttcc tcttataagt ggagctagta
     8761 tggttatttt ttgatctgta aaatgctcat acacatggtc tatcatctgt acttcagagt
     8821 acctaaccat cttcttgcca gtcacgtatg aatgtgcttc acctattcta gcgaataaca
     8881 atcgtaggaa gtcaagtatt tcggtaacag tacctacagt agatctggga ttttttgatg
     8941 tggtcttttg ttctatagaa atcaccgggc ttagaccgga aatgtactca acatcgggtt
     9001 tttccatact gccgataaac tgccttgcat aggatgaaaa agtatccata tatctgcgtt
     9061 gaccttctgc gtatattgta tcaaatgcta aggaagattt tccgctgcca ctgatacccg
     9121 taataaccac taacttattt cttggaatcc tcagatcaat attttgaaga ttatgctccc
     9181 ttgcaccggt tatctcgatg tattctggtt ctactaccat attatttaat caatgaactg
     9241 gatgatttct ttgaatattt ttgccaactt tggttctgtt tcattagcaa ccgcaatgac
     9301 cgactcgtgt gtagtttctt ttatagccga taatgggaaa catttgtttg aaacaactga
     9361 tacaccgaat actcgcattc cgcaatgtct ggctaccagt acttcaggta cagttgacat
     9421 acctacaagg tcaccaccga tgacattgat aaaattatat tcagacggtg tttcgagatt
     9481 tggtccctgc aatgcaacat aaacgccttg atgacagcga atattatttt tattagctat
     9541 ttctacagct ttttcaatca gattcttatc atacgcatct gacatatccg gaaatcttag
     9601 accaagacgt tcatcatttg gccctcgcag cggattttca ggcatcaaat tgatgtggtc
     9661 tttgagaaag acaatgtctc cggcttcata agcggcattg acactaccgg cggcgttact
     9721 gatcagtatt gtctctaccc caagttcttt cagcaccctt acaggaaatg tcacttgttg
     9781 catggtataa ccttcgtagt aatgaaatct gcctgcaaga atcacaacat ctttttctcc
     9841 aagtctgcca aataccattt cacctttgtg cgacgctaca gtcgaatggc taaaccacgg
     9901 tatagcagca tatcggatta tcacttcatc ttcaatttct gatgctatat tacttagccc
     9961 cgtaccgagc accatcccga ttttcggttt cacgttaagg tgggtgacta tgtagtctgc
    10021 agcttctttt atctggtcaa acattcgatc attcataaat gtaggcttag tgtattatgg
    10081 gactgaaaaa taatttgttg attattcaac ttgtaaaaat agtataaaac cttggtttat
    10141 ggatggaaat ttatacaata atggacattt gaagctatct aaaaagtaaa tttatgtttt
    10201 ttgaataatg tattgtaata gtaaaagtta atctaacata ttgtagtttt gagccttgtt
    10261 ttctaaatta aatggtgaaa tattttcttt cggtcttatt tattctatca ttttacgagc
    10321 ttagggcaga ctttttcaga attcttgatg atgaaacaga agctttgcag tgccggatag
    10381 atatgattga gcaggcaaaa agtgaaatcc ttctgtctta ttatatactg aatgatgatc
    10441 tggtaggcat gaccatcttt tctgcactcg tggaggcagc tagagacaga aaagtaaaag
    10501 tttgtctgat agtggacgcc gtaaattcaa aaataagaaa accggtcatg cgttatcttc
    10561 aagacaacgg cattgaagtc agggtattta aaccaggaaa tattttacaa cccatatcgt
    10621 atgctaaacg aatgcacagc aaacttttcc ttaccgattc cgaaaacctg atagtaggtg
    10681 gccgaaatat caaacgggaa tattacagct tgggcaagga ttttaatttt cttgaccgcg
    10741 acgtatttgt aaaaagcaga tcagccaccg aaactgccag aaaacatttc tattcgatct
    10801 gggataatga aaaaattgca aagcacaaag atctgaaacc cattagtgtc gatgaaaagt
    10861 tggaaataga aaataaactg agactggcaa gaataaaaat ccaggatctt aagaatatta
    10921 aattcgccac atctacagac tggacactca accaaaaaca aacagataat ccacctgtat
    10981 tcgaacacga taactatcac atatataaag gcggtaaaaa aattgaactc gactataagg
    11041 atacagatga aggcagtacc aaaagccttc tcaaaataat taaccaagct cataagagta
    11101 tcctgatgga aaatgcgtat gtgataccta ccttaaaatg gcgtaaagcc ctgaagaaag
    11161 caatagaccg tggtgttaag gttagaatac tgaccaattc ggcccaaaca agtgacctta
    11221 tgatagctca ggctgcatac cgcaatgccc gaaaaaaatt attgaaaatg ggcatcgaaa
    11281 tctgggaata ccagggcccc aaaaccttgc ataccaaatc aacggttatt gacgactcta
    11341 tatcgatagt cagtagttat aacctggtcg ctttctctga gcgctggagt actgaggtgg
    11401 cagtttgggt ggccgatact accatagcca gataccatac tcgcatcatg gagcaaaacc
    11461 tacagcatgc actacggata gacaaaaaca acaatcaggt aaaacaccgt atgcccgatg
    11521 acacaaaaaa actcacttgc cgtaagcgta ccagcctgtt catcaaccga tattccattg
    11581 cctggacaat gacccttctg taaaaataat ttcttaagac cattaaacca tactgcattt
    11641 ttcttgtcaa aagaacaaat cggataatca agatggctga agtaaaattt agtgaagacc
    11701 atgaaattct cagactgctg agaaatcaag atacatccga taaaggtttc agactattga
    11761 tgggtacata taaggaacgt ctctattatc acatcaggca aatggtagga aaccacgaag
    11821 atgcagatga tgttttgcag aatgtattca taaaagtgta cagaaatgtt caaaacttcc
    11881 aggaaaaatc aagcttgttc acctggttgt ataggatagc tacaaacgaa gccttaaatt
    11941 ttctgaacag taataaaaca aaacaaacag tagcactgga tgaagtggca ttcaacatta
    12001 caaacaatgg ttcagaagaa tttcaggatg aaaatgaaat acaaatgaag ctactacaag
    12061 ctgtaaacat attgcccgat aaacaaaaac aggtttttaa cctcagatac aacgaagaaa
    12121 tgtcttatga agaaatatca caaatactag aaacttctgt gggtgcactt aaagcatcat
    12181 accaccatgc agttaaaaaa attgaatcat atattaaagg aactatataa attatgaaaa
    12241 aggaaataaa agacgaactg aaggaaatct catcttttct ttcggaaata caaaaaccac
    12301 cagtaccaaa ggtaccggag caatatttcg aaggctttga agccagaatg atgcgtcgta
    12361 ttgaaagtga aaaatctcca tctgagcaaa gaagtaaaga aagttggttt tctactattt
    12421 ttacctattt caccaaaccg gccatagcct tatctttcgg agtacttgcc ttgttattga
    12481 ttatgacaaa atatatctca actgacactg ccacacgtga tatatacgcc gatgagctgg
    12541 cacaggtaga taagcaggag ttactcagtt atattgatag ccacatagac cagtatgata
    12601 cagaaatatt acttgatgat aacacagatt tgattgaaaa ttttgacaca ccatacccgg
    12661 atgatatacc cgatacatta taattattat tagacgaaaa aaattgttaa cctttaattt
    12721 taaaacagaa atgaagaatt tattgatagt agccatgttg tttttctcaa cacagtacat
    12781 actggcacaa gataacaacc cgcctcagca aggaaaaaga ggagaaaaaa tagaatcgct
    12841 gcgaatagct tttatcacag acaagctgca gttaacacct gaggaatcaa aacagttttg
    12901 gccggtgtac acacagtaca ccaaagactt agaacaattg cgcagtgagg ccaaacgggc
    12961 caaagctgaa gtcggaatgt cagatgcaga agcagagaac atggttaaag acagattttc
    13021 aagacaagaa cgaagactcg aactcgacag aaagtattac caggagttta gaaaagtatt
    13081 gccagctacc aaagttgctt tgttttttga ttctatcaat cagtttaacc agaaggtact
    13141 caaagcctac aaagagaaaa agagagaagg caacagacca ccaaggcgac gtttctaagg
    13201 cagattaaag cccaaacact tcaaaaaaca gatgggtttt gtaattttgc taaactttgg
    13261 aaagaaatac tttagcccac ttgtccttgt tttcagttgc gttcatttat gcagccaatt
    13321 ttagtattgc aaaaatagta atgggaggtg gttatgtgcc accttccact tttatattgt
    13381 gcagaataac ttcagcactc gtattgttta cagtttacca ctacttattc gttaaggaaa
    13441 aaatacagcc tgacgacaag aagcctatga tttggttggc atttttcgga gttgccgcca
    13501 accagctttt gttttttatg ggtctcgcac ttactacgcc catacatgca gcattgatta
    13561 tgaccatcac acccatatcg gtagtgatag tagaatccgt actccgcgaa tctcctctta
    13621 ctttcaataa agtgtttggc gttttaattg ctttcactgg tgctatattc attattttgt
    13681 caggtagtga tatcagtttt gatggtcaaa caatgttagg tgattttttt gtatttgcca
    13741 atgctgtatt ttatagtata tatctggtga aagtaaaacc tcttgtgcgc aaatatcatc
    13801 ctatcaccat caatagggtc acatttctat atggattttt tatggttttg ccttttggct
    13861 tcccaaatat tacaggcata caatggggcg ccttcgacac tcgtatctgg ctttctttct
    13921 tatatgtact tatttttaca acattttttg cctattatct caattcttgg gcaatgctga
    13981 tagttaaacc atcggttgtg ggcgtgtata tatatcttca gccattgctc acaatcatta
    14041 ttgctataat tattggtaca gacaaaccag atattaatat cattcttgcc ggattattaa
    14101 tctttacagg tgtattttta acaagttata caaaaaatat taagaaagac acatagtaat
    14161 ataacatact tacaagatat ctacgaatta tcaatagaac ttaataagca taataactat
    14221 tcaaatattt aaataatttt gccattaatc gctgacgaac aatgatttat gtatgcctaa
    14281 ggttttttga taatataaga acaactagaa atttttttta aaattagctt caaaaatatt
    14341 cggatacttc atcaaattac ccatatattt gttcgataag agcatattga cataacatga
    14401 aaaggaaagg gcagagatct gaacagacac caagccaatt ggtatcggat tatactataa
    14461 tgctggaaaa cggcgaaatc gattttatta aggaagatga ttggcaaagt ctgattgatt
    14521 attttgtaat taataacaga ttgtcagacg cacttgaagt ctcacagaaa gccatcgaac
    14581 agcacaattt ctcaatagag cttcatatca atcatgcaag aatgctgcta gccaatgaat
    14641 tttatcgtga ggcggtcctt tttgtagaag aaaaatctta cttgtcaccc aatgatccgg
    14701 aatttatcct cgtaaaagca caaggtatgt tgaacctggg tgaagaagac tgctgtcagg
    14761 agctcatttc ttctattaag aaagatgcag acaacgactt gctaacagat attttgctca
    14821 ccgaaaccga tttgtatgag cgtgcacaag aatttgatct tatgtttgat gcattacaga
    14881 atattctgct catccacccg gacaatactg aagccctaga acgtatctgg ctttgtacag
    14941 aattgtcagg ccggtacgaa gatagtattt tgctacacca gaagataatc gaaaatgact
    15001 cttactcatt tcaggcatgg tataaccttg ggcaggcata ttttagcatg gaacaatatg
    15061 accaagcagc tgatgcattc gaatatgctt atttgataaa tgaagacttc gaatttgcct
    15121 atcgcgacag aggcgagtcg ctcttgttgc ttacacatta tgaactagca ctcaattgtt
    15181 atcaggaagt aaaagaaaga tttgtgccgg atgccgacct gtattgtaaa atcggccaat
    15241 gttatgcatt tcttgagcaa cccgaactgg ctatagatca tttcagtgtt tctctgcaga
    15301 tgggtaatac caccggtgaa gttcattttc acttgggtgt atgcctggta acgctcgaaa
    15361 aatttatagg cgccgaacaa tcattgcgaa aagcagtaga acttgaacca gaacgagagg
    15421 aatatcatct ggcactggcc gatttgtatt ttcaacaaga aaattatgaa ctagcatcta
    15481 tttcttacca aaaggctgtc gatcttgctc ctgaaaatta ccttatctgg tgtcaatatg
    15541 cgggtttctt gttattgact catggcgatg aagctgctat ggaagctatt gatgaggcat
    15601 tattctacag tgattctgcc gagttgcact atctaaaggt ggtgtgtatg ctctcagcag
    15661 gcaaaagaca ggaagccatc attaatctta tgaaattaat ggatacttat cctgagcatg
    15721 cggattatgt ttatgaagta atgcccgact taaaggacga tgcagaggtt acggccatca
    15781 ttgcaggcct gtagtccacc aagagcattc atttaatcat tatttactac acgtattttc
    15841 atccagacat cttgttcggg tctgtcaccc ggtaaagtct ttacggcagc tattttgtct
    15901 ataatatcca agcctttgat aacttcgcca aatactgtat aatccatatc aagaaatggt
    15961 gtgccgccaa gtctctcgta tttttctact atatcttttg gataattaaa acccttagct
    16021 gcttctgttc tctgcaagct tcttgggtca actggtctac cctgcacaat ataaaactgt
    16081 gagcccgaag aacgcttgtc aggatttaca gcatctgaca tgcgagctgc tgctaatgcc
    16141 cctttagtat gcgctagaga tgaattaaat tctgccggaa tggtatagcc tgggccacca
    16201 cttccaagtg gtactcctgc tgatgccccc tttgaagtag ggtcacctcc ctgaatcata
    16261 aaaccattga taacacgatg aaatagcaga ctgtcataaa atccctgccc ggcaagtttt
    16321 agaaaattgt ctctatggtg tggtgtctga tcgtacaata ctgcagtcat cgtaccgaat
    16381 ggtgtttcta tctctacagt gcaatgttct ggtgcagaaa tttctaaagt catggttttg
    16441 ttgttttgtt ttttaccttt aatagcagta agcgtaacag tataagtgcc tgcagacgca
    16501 tattgatggg ttgggctcac ctcagtggag cttctgccat ctccaaaatc ccaactgtac
    16561 gatttggctt tttttgatgt atttttaaaa taaatattac tgaatgcttt tttgttagta
    16621 ctggtacatt cgaagtcagc ctgttgggcc aatatgctaa atgaagcagt agtcaataac
    16681 agaagaaaga tgcttttaag attcatactc agaaagaata attaattgat gagcgaataa
    16741 gaaacgtaaa taatgcagaa aagttgtagt tgaatcatga ctatcatttt gtgtaaaaca
    16801 tacacttacc acgagttttc ttttgccaga acatcaaaat acttgattgc ctcagccttc
    16861 aagagttttt cctgattgtc cacaccttct gccgacactt ttcttagttg tttgaaatgt
    16921 ggccatccgt cttcatcacg acctatgaat tcgtaataac catcgtgact gagcagacta
    16981 catacaccta tgtgcatcag atcttgtttt tcttcttttg tccatttctt ccgcggcata
    17041 ccaagctctt gaatgcctat aagtataagt acagtattca tatcgggtag cctgtcgtta
    17101 ccaagctgat ctttgataag gtgctggatt ctaagccagt caaaatctaa ttgccagtct
    17161 tccatttttt atatttaccc tttattttct ctaattacta tacaaatatt ttaggaaatc
    17221 gtttttactt gtttcacaaa tatttttatt tcaataccct attttcaatc accatattta
    17281 aaaagtgtcg atgaacgaga ggccattttt gccggaaaat aagatgccag aaaaccaatc
    17341 atcgaaacaa taataagaat agctgctata tctgttattt ccatttgtac cgggtacaca
    17401 ttcactgcaa agccttcagg tacacctact atccctactg ttttttggag aatatacagt
    17461 attactgcta cagccagacc cactagaaaa ccaacaacag ataacaacat accaaggttg
    17521 agaaaaatac tgaatacctg tttgtctttc atgccgagtg atttgagtgt tgccaggtca
    17581 gatttttttt ctaataccag catccacaat gcacatgtca tgttgaatgc tactagtagc
    17641 agagtaagtg aaagtatagc aaaacttacc catttttcga tgttcatgag ttttagaaaa
    17701 gcttcttcct gctgatatct gtttttcaca tcaaattttt ctcctacaac tgcctgtata
    17761 ttcttctgta aggtcttgac attttttttg ttgtctatct tcagttcaat ggcacttaaa
    17821 ctattcggtt tgttgagtag ttcttccaca aactcaagtg aagtgatcac atattcataa
    17881 tccacatctt gttgtatcga aaacaatgct cccggataca aaaagcgcct ggtaaatggt
    17941 tgcgatatta ttcctgcttc atcttcattt ggcgaataca ccgaaatcgg ttctgacaac
    18001 tgcgaaggca ttgtaccaag tttattagca agcccaaaac cgataatgcc ataatccgtt
    18061 ttatccttcg taagcatgta ttctccttca agtattgccg aatcaagtga tgctacctgc
    18121 ctgaaatgct tgtcaacacc ttttaatgta ccaaaatcct gaactttttc actttcgaac
    18181 aaggcttttt cttccagaac ttttgaaacc gccaatacgc cttctgtgtt ttcaagtttc
    18241 ttaatcatca ctgaatcacg ttcgaaaaat tttccctcac gtggtgttat tttgatatca
    18301 ggattgaact gattgagcat tttggttatc agtccttcaa aaccattaaa gactgacaat
    18361 accagaataa gtgccgcagt acctatcgat ataccaagta tagaaatggt actgatgtaa
    18421 tgtatggcat tcgccgagcg cttgctgtaa aaatatttcc ttgaaaatat aaaggacaga
    18481 gttctgttca tgtcagagta atgattcgta tatggcagtg tgcatccgtt cgcgataatc
    18541 ttctttgacg agtttacgat tgttcatagt agggaaagtc ctgtttgaca agaatacaaa
    18601 gatgatgtca ttttcaggat cagcccaaac acaagtaccg gtaaatccgg tatgaccgaa
    18661 tgttttatct gatgccagtt ttgacatatt tgaaggggtc tgcctgtcgg taacaccaag
    18721 gtcaaaccca agcccgcgcc ggttgcatcc atcacatcgc gaagtgaata accttactat
    18781 ctgcggtgtc agatattctt ttccgccata agaaccttta tttattagca tctgcataat
    18841 aaccgccaga tcacgcgaat ttgaaaaaag tccggcatga ccgctaactc ctccaagcat
    18901 ggcagcaccc atatcgtgta cgtatccctg tattttccta aatctgaagt aattgtccat
    18961 ctcagacggc acaatcctcg atttgtgaaa acggttgatt ggtctgtacc cggtggtctg
    19021 caatgccagt gaatggtaga aagatttgtc agcaaatttg tcaatagtag ttccggtaat
    19081 ccgggtgatg gcctctgcaa gtaggtaaaa tcctagatca ctatagcggt agtgaccaac
    19141 attgggcaac tcactattca ctatttcatt atacataact tcgcggtagc tattcttcat
    19201 gtaaaggctg tccgtcactt ttattgaaaa cacactggtt cgttttcttg aatatacctc
    19261 aggcgatgga aatgatttat tacctacacg caccaatgtt ttttcataaa atggtatcca
    19321 tgacctaaga cctgcccggt gagtcatgat atctttgata agcaaatatt ccttattaga
    19381 ataacacaat tcgtcaagat aagaagacag tggctcgtaa atgctgattt tgccttcttg
    19441 ctgcaatttc ataacggaga gtgtagtggc ggctatctta gtgatagaag caatgtcgta
    19501 tatgctgcgt gcagtcactg cagttgtttt gtcataagtc gtataaccaa aagatttgtc
    19561 aaacacaatt ttaccatcct tggctaccag tacctgacaa cccggagttg attttatatt
    19621 gatagcatca ttggcaagca aatcaatagc attgagcttt attgaactga gtccgacacg
    19681 ttctggcaaa tcatatccta ttctgattgg tttattgatg ttgaggccta atccgtacct
    19741 gcttttgaac gaagatgtta ccggtagctt ccctttgcag gtagcaccac ccaataatac
    19801 ctgtgctgcc aaatcctgaa aatctggtgt gtcttcataa gcttgaagaa ggtatggtat
    19861 ctggtcaaaa ttttttagga tatagggcga accaaaattg acaagaatca ctttcgatac
    19921 agctgcaata tctctcacca gatctatttc gttagcggtc aggttgtact tgttcttcgg
    19981 attgttggta agaccatgaa ggcttactat gacatattgt ttattgcgac aatggttgat
    20041 gatttgtttt tttctgtctt caccgtctgt tttaccaaat gtgtaataag agcattttac
    20101 gtatttgtct attgtgtttt ggaatgtttg gaccgacttt gtacctacag ctacggatgt
    20161 gagtttcaga tcttcgatat tcttgagcgg aattttatcc gacggattcc tgacaagagt
    20221 gagagcattt tctattaagg tacggttgag tgcgatggct ttttctgaat taagatcata
    20281 tgcaaggtta aataagttga ttggctgata gtttgacaaa ccaaaacggt attttgcctc
    20341 aagtaccttt cttactttat tgttgatgtc ttcttctttc agttttccat tttctatata
    20401 ttccttgatg gtttttaatg cctgtggtac actttcgggc agtagcaata catcatttcc
    20461 tgccagcaat gcctctgctt ccacttcacc cgattcgtaa ttttttgtaa cgcctttcat
    20521 ctcaagacca tccgtaaaga ttaaaccccg aaaacccatt gttttgacca aaagattatt
    20581 gatggctttt gctgaaaggc tcacaggtac tttcggattg ttttctattg ctcttacatt
    20641 aagatggccg gtcattacag attgtacacc ctgactgatg agcgttttga atgggaacaa
    20701 ctccagtgta tcgagccgcg ccattggttg tgtgagtacc ggtaggtcgt agtgtgaatc
    20761 aacatccgtg tcaccgtgtc caggaaagtg cttagcgcat gccatgattc cattatcctg
    20821 caatcctttc atataggcag ttgctttaac ggctacgttt tcgcggtttt cgccaaaaga
    20881 gcggaaatta atgaccggat tgtcgggatt gttgttgatg tcggctaccg gggcaaaatt
    20941 gatgtgtacg cccagacgct tcaactgaag ggctatctct ttgcccatgt cgtttatcag
    21001 tttttcatct cttatggctc ctaatgtcag tgattttgga aaactcatga cagaatcagt
    21061 tagtcgcata ccgagccccc attcagcatc catgcttacc atcaaaggaa tgttggcaag
    21121 tgactgataa taattggtga ggttggcctg cttgccgggt gtaccctgga aaaaacacaa
    21181 tccaccaacc tggtaatttt taatcagatt ggcaacatcc tgttcgtatt caggtgtttt
    21241 gttactgtgt gcacgtacca tcatcagctg gcagattttt tggtcgagcg ttagtgtgtt
    21301 catcagtgaa tctacccaga catccgattt ctgaacattg tagggcattt tgttgaatac
    21361 aatagaattc gactttggac tgatacatag aaatccgatt agtaacagtg cagataatat
    21421 tttctttctt ttcttcaaca ttttatctga taggtttaca ggcattattt gctttcttgc
    21481 ccttttgatg caagtgcggc atatttatca ccattgactt tatcaccggc attatagtaa
    21541 gcatggctta aattattaaa cgcttcggat accggattaa tttcagcagc acggacaaaa
    21601 tattcgagcg ccttttgatg ttgaccactg atgccattgg ctaccccaag caaacggttg
    21661 gtgtcatagt cctgtgggtt caattcgtac gccttgttga ggtattcaag tgcggtcttc
    21721 aggtcaccct ttttttctcc gaaatatttg cctgcttccc tgtatgcaag gtgcaggttt
    21781 gtcagggcat ccttgttatt cggataaagc gcgaggcatt tatcgtattg tttgattgct
    21841 tcatcgtatt tttccgataa aaagtaagca ttgcctagca ggagatatgc attgtggtat
    21901 gtagggtgga tagaaatggc tttttccaga tgcccaattg cttcatttat cagtcgtgtt
    21961 ttctcggcag taccttttgt ttttcctgct ttggcaatga gctcaccacc ggctgaattt
    22021 tgtagcttgg cgctattgac agaagtaagt acatcggtaa tgaataaggt atagttatct
    22081 ttccaaactg tattgcgtgt aaaagtcttg aaactatata gcagacagat gataccggta
    22141 atggccaatg cggcattata attaagggat tttggatttt tattgaaaaa cctggaagca
    22201 agttgatgaa tgccgtatcc ggcaaaaatg caaaaaccta ccgaaggcat gaataaaaac
    22261 cgctcggaca tattagtacc aatgggaaac acgatgttcg taacaatgga tattgttatc
    22321 agataataca atatgccaaa tgcatatagg atgtgttcct ttattttttt gaatacaaaa
    22381 acaagcatcg caagataaat caaaccggat aagataaccc cgatatctcc aaaactcatt
    22441 atgtcaatgt gtcttggata ataatcatgc gtaagcggat gtggaataaa aagcaatttg
    22501 acatacaagc ccagtgtaaa taaaatggta gcaaattttt cgccagtcgt aaaagctatg
    22561 tattgattac cggataattt cagaaaagga ttgttcatca gttccattgg ttcgttgccg
    22621 aatgccaaac caatggcttt ggtacggagc atgaaaaata ttgctacaga aagaatcaat
    22681 ggccatatcc ggtatatacc ggtacttggt ttttcatcat aaaatacaaa aaaacacaaa
    22741 ggaataactg ccagaaaggt aatggcattt tctttagata acaatgcaag caaaaaaaca
    22801 gtaatggcag caagcacatc tttaagtttc ttagcttctt tatattttat taaaaagcat
    22861 agagtaagca ggctgccgag taatgctagt atctcatcta cccccttgat gttggctacc
    22921 gcttcggtat gtattgggtg gcctgtaaat aatactgcgc tgatgaatgc aatgattagt
    22981 gcctgcgaag atttaaatct tcttgaaaaa agccagttta acacattgaa caatactaca
    23041 caagtgatgc tgaataacat tacagtgaat aagtgaaagc caaacggatt gttaccaaaa
    23101 aattcataca atacagcaaa gactatcaat gaaaatggtc tgtaacggcc tcctgccacc
    23161 aagttggctt ttccggcttc tttaaaaaaa ccataaaatg tatcgtactc aagtatgccc
    23221 gggataccct ttataccttg ggtggtgtac atattgtcgt agataacaat ggcatcatcc
    23281 tgagtaaaat cgtaactcag agtgttagcg tacaacaaaa aacccaacat aaaaatgata
    23341 acccttagtg tacggatatt catccaagcc ggcaattctg tcattgaccc cattgtagta
    23401 tttgcgatac tttttgccat tcaatttgtg tattaaaagc tttgtttatg taagcatcgg
    23461 gcaaaataac tcaatttttt ttaatttttg tagtaaatat tgtactgata ttactttgta
    23521 tttgattcat attgagtatc aaggatatat ttgtgtgcat ttaaaggcca aatagtttaa
    23581 ctttgtactt taaaacttaa agatgtcaac acccggttca gtagcatttc acacattagg
    23641 ctgtaaatta aattttgctg aaacctcttc tatcagtagg tcatttaagg atattggttt
    23701 ttcaatcagg aattttgaag aacaggcaga tttttatgta atcaatacat gttctgttac
    23761 agattttgcg gatcgtaaat gccgtcaggt agtcaggcaa gcactgaaac gtaacccgga
    23821 tgcaaaaatc attgtcatcg gttgctatgc tcagctcaaa cctcaagaaa tagcagaaat
    23881 ggaaggtgtt gaccttgtgt tgggtgctgc tgagaagttt aacgttgtac agtacataga
    23941 catgttgcag acatctactc aaaaaggact ggtgtttgcg agtgaagtga atgcggccaa
    24001 tactttcatc tcttcctttt ctttcggtga ccgcacacgc actttcctta aggtacagga
    24061 tggttgcaac tacaaatgta catattgtac catacctcag gcccgtggcg aatcgcgcag
    24121 tgatactatt gataatgtcc tgaaaaacgt tgatgatatt gcagctaaag gctgtaagga
    24181 aattgtactc accggagtaa acctgggtga tttcggcaat ggtacggaag taatagaagg
    24241 taccagacct cagaaagaag caatgtttta tgatctggta aaggagctcg ataaatgcca
    24301 gggaatagag cgtttcagaa tttcttccat tgagcccaac ctgcttaccg gcgaaataat
    24361 tgattttgta gcagcctcaa ccaagtttgt acctcatttt catattccgc tgcagtcagg
    24421 aagcaataag atactccggc taatgcgcag acgctatctg cgagagttgt acgcaaacaa
    24481 ggttgctgaa atcaggaata aaatacccga tgcatgtata ggtgttgatg ttattgtggg
    24541 ttttccgggc gaaacaaacg atgattttct cgaaacctac cagtttatta aagatttgga
    24601 tgtttcgtat ctgcatgtat tcactttttc agaaagagaa aatacattgg ctgccgaaat
    24661 ggaaggtgta gtatctgtgg aagagagacg tagacggaat gaaatgctcc gaatattgtc
    24721 cgaaaagaaa aaaagagctt tttacgaccg atttgtaggc aaagaaaaag atgtattgtt
    24781 cgaagcacac aaagacaaac gaaaactgac aggctttaca gataattatc tgaaagtaga
    24841 gttggagtat caagataatc ttatcaatac cataggtaaa gtcaaactca taagtctgaa
    24901 taatgaaggt gacttcattg ctgaacgagt tctacaacaa aagttatcag ttgcaactga
    24961 cacaatatca gaacatgcat aggttattca ttatttttat tctagcgctt aatacttatg
    25021 ctcatgcaca gatttatgtg acaccaaccc tattgggcaa actttcggat gacctgaaag
    25081 aatcatcagg gttgctgata cagagtgccg ataccttatg gagccataat gattcgggca
    25141 atagtgcaaa agtatttgca ttcgacaaaa caggcaaaaa aataaaagac agaaaattta
    25201 ccaaaatatc taaagaagac tgggaagata tatgcagaga cgaaaacggt tatgtgtacg
    25261 taggcgacat tggcaataat gataacaatc gaaaaaatct gcttatactt cgtttctctt
    25321 cggggtttta tgataaagat gaaccactcg tagttgattc attgcagttt caatacgaag
    25381 accaaacact ctttccgcca ccatcttcat cattaaattt tgattgcgaa gcattcgttg
    25441 taatgaatga caccgtattt ttatttacga aagaccgtac gaaaccatac atctcgcaga
    25501 cattattgta taaatttcct gctacacccg gacaacatac cgctaagtat gtatctaagt
    25561 ttatgaccga tgtgccactg tatcttcagg gttctgtaac cggtgcaggt ctgagtcctg
    25621 acggaaaaaa acttttgtta accggatatt taagaatgtg gctgttcaca gattttgaag
    25681 ggagtaattt tttcgatgga aagcttacag tcatgcagta taatgaatat tcccaaaaag
    25741 aaagtgttgc atttgtggat gaatgtactg tactcatgac cgatgagtac aatgctgaac
    25801 tgaataacgg cggcaaccta tattcgctca atatttgtac caccgtagca gttgatgaag
    25861 ctacaacctc agcgtcgcct gttgtatatt tcaatcacgc ccaaggtatt ctgacagtga
    25921 gtagggtgag tgctgcctgc gaactgatac tctacagtgt taacggcaag acagtggcta
    25981 gggaaaaaat tcctgtcgga tctgaaaata ttcagattaa gactttgaac tctttagcac
    26041 ccggcatgta tgtctataca ataaaggaaa aatcaggttt ttattccggc aaattaatgg
    26101 tctattaatg cccttttctg aaactgtcaa tatctgtgga aaggctcagg tgatgtgcct
    26161 tgcctgtaag atggtatatg gtcattccat aatcaaacct gaatttgcta atcttaaatc
    26221 cgaggcctcc gctgaagcct gccaagcttc gataattatt gacgctgagc tcttttctaa
    26281 gcaagtggtt ataaccaata cgaagtcgaa gattttcctt cttaccaatt aagaattctc
    26341 cattgaacac aaaatgtctg aacagattat ctataaatat agaggcatca cttcgttgtg
    26401 cgttttggtt cgtgaatata tcattttctt tcacttgttc aggatcatca tacaacacat
    26461 tccaacgttg aagattggta taaactattg aaaatctgaa aggtaagtgg gcaagtcttt
    26521 tactgatacc agcctgaagc tcgtagggta gtcgttcggg tttgtcattg aagtccgtaa
    26581 gctgcgtgcc tatgttgcgg catacaaggg cgatagtaaa gtttttattc gggataaagt
    26641 aacttgctcc gatatcggct gccaggcctg ttgaactata gctttctaat tgtgaagtga
    26701 tgaattttag gttaacacca agtgagagat tttcgtacag gtttcgtgat gcgcctacta
    26761 tgagagcgta atcagcggct ttgaacgaac cggtgatgtt gccatactca tcagcttgtg
    26821 taaaattgcc atagttggcg tattgtacac ccgcatgaaa atggtaaggg atggattttg
    26881 gagaaaaagc gtaggtaaat gtaccatctt gtagcccaga atagaaaaat ctgtggttga
    26941 aactcagttt gtcgttcgaa ttttcgttta gcaaagaagg attttgcata gcaagagcag
    27001 catcatcatc gcgtatggca ataccgttgc cggcaagagc agttatgtgg gcatcaggag
    27061 ggttccatag gaatttgtaa acacctacac cacctaactg agcagataca gttgaagcac
    27121 gacaaacaat gaatgtcaaa attaagcatt gaaccaaatt acctcttaaa atcttcataa
    27181 gcttttttta cccagattgt attgcactcg cccattttac aatccagttt ttttattaat
    27241 tcgccctcct cattatatac cttcatttca ccatcttcat ggtcgccatc tttgtaagtt
    27301 ccttcgtatt ttatttttcc gtttttgtgg tattccttaa atgggccatt ttcattattt
    27361 gctaccatgg ttacttcttc tttcagttca ccggtatcat agtaagtttt ccagatacca
    27421 ttcatggcat tgtgggagta atgaccttcc tgaaacaatt ttccattttg atgaaattct
    27481 ttgaatgtac cctcatacga cccgtttaca aagttttctt ctgttcgagg tttgccatct
    27541 gcgaagtaag tatatcgctt gccgtcttgt actccgtttc ttatttctcc cctttcggtg
    27601 actataccgt ttgcgttttt atcttcataa acaccatcgg gtcttgaagt gtcgtggata
    27661 agggatgaat caatgtccgg atccggttgt acttctgtgg cagataaagt gtcgttaacc
    27721 gttgggttat cgggttgccg accgatattc ttacaaccaa aggtaaaagc cgtaaatatg
    27781 ataagtaaaa agtactgaaa tttcataaaa acaaaagctt tctattcaga acgaaaaaat
    27841 ttttaccaat tgtataaaaa acgttaattt atatcaaata atctctctgc cgatgctttc
    27901 gtaatggaat cctgtttcgg ccatttgttc gagttcataa acatttctgc cgtcgaatat
    27961 tattttatca gccataagtg aggcgagtcg ttcgaaatct ggattgcgaa actctgacca
    28021 ttctgtgata atagccaatg cattagcatc ttttgctgct gcgtaggcat tgtcggtata
    28081 gctaagtttg tctccgtata tttttttaac attgtccatt gcctcaggat caaatacaga
    28141 gatgtgtgca ccagcattca acaaagcatc aataacgtag agtgccggag cttcgcggat
    28201 atcatccgta ttgggcttaa aggccagccc ccatactgcg atttttaatc ctttgagatt
    28261 gtggttaaaa taagtggata ttttttcaaa taagacgaat ttctgttttt cattcacagc
    28321 cattacagac ttaaggattc tgaagtcata tttgtattgt tcggccgtgt ggtacaatgc
    28381 aagaacatct ttaggaaagc agctaccgcc ataacctaca cccggaaaca gaaagcgttt
    28441 accaatccgg ctatcgctac ccatacccat gcgcaccata tcaacattgg caccgactct
    28501 ctcacaaaga ttagctattt cgttcataaa agagattctg gtagccaggt aggcattggc
    28561 tgcatatttg gttatttcgg ctgacctttc atccatatag aatatcggat taccttgtct
    28621 tacgaatggt ctgtacaact ggtccatgta tttgcgggct ttgtcagatg atgtaccgat
    28681 gacgactctg tcgggtttca tgaaatcctc cacagcaaga ccctcacgca agaactcggg
    28741 attggaaaca acgtcgaaca aatctggtga cgtgtttttg gctatagccg catgaaccaa
    28801 ttcggcagtg cctacaggta cagtactttt atcaacaatg attttgtaag tatcaatcat
    28861 tgttgacaaa tcatttgcta caccaagtat gtatgacaag tctgcagaac catcttcgtc
    28921 cggtggtgtg ggcaatgcaa ggaaaataac gcttgactgt tctactgccg attttaggtc
    28981 cgatgtaaat ttcagtctgc cttcttctac attcctgtta aacaaaacat caagtccggg
    29041 ttcgaaaatt ggtatattcc cttctttgag actttcaagc ttattgaggt tgttatccac
    29101 gcaaatgact tgattgcctg tttctgcaaa acaagtgccg gtcacaagac caacatatcc
    29161 ggtaccaact actgcgatat tcatatattg tattaataaa taaatgcaaa aatattaatt
    29221 taatacgttt gctgaaaaaa ataaataaaa aacgaaaggc tgcccaaaac atttgagcag
    29281 ccttttggcg aaagccaacc gaattttagt tgaagaactt gtaacggttg tccttgattt
    29341 tgtatgtttt tcttagtgct tccattactt taggcgccac aagtgatgca tattggctgc
    29401 tggtagaatt tttaataaga gccatattgg gtggtactgc accaggcgtt ttacttattg
    29461 ggttgatgac aaatacacca ctattaccga ttatagggtc agatgtggta ttgacaccaa
    29521 gtttgaaggc tttggcgaga acttttggtt cattaccaat acctgctaca aatggtgctg
    29581 caaaattaac acccgtagca gaatcaactt tggtagtgaa gtttgcggcg atggcattca
    29641 aatctttggt agtaattttc gctttaatca tctcaccttt cttgcggttt atcacttggg
    29701 tcttgatatc gtcttttaca gcagctacat caggcatacc ggctttttga attcctgaaa
    29761 gaccagctac tacatatttg tctatataaa actcttgtgc atttttgaat gaatatacat
    29821 tcggagcgat atcgcctatt ttagcttcac cggtaaatgc ccatttgatg atagaataag
    29881 aagcctcacc ggcacccaaa acacctagac tgtagtcgtt tgctttcagc ggaatagact
    29941 tgattagttc tatctctttc gagcctgcag ttgacttttt catatcatca acagatttat
    30001 tttctgctaa gaatttgttt gctttgtctt ctgctgcggc ctgagtttct tcgcttggta
    30061 caagtgcctc tctaaataat gagaacttga caccaggctt gttatctacg aatttctgcc
    30121 cggttacttc tactagatga acaccgaact gagtttttac agtatagagt ttgttaggta
    30181 cagctttatt aaaaataaga tcgttgaact ccggcaccat ttggccttgt gccacgtaac
    30241 ccaagtcacc gcctttgtct cttgatgcat cttgaccaaa acgcaaagcc aaagaatcaa
    30301 aactgttttt gcctgtctca attactactt ttagagaatc tattgttttc tgagccttgg
    30361 cgatatcacc aggggtagaa gcctgtaata gaatgtgtct tgactttaca gagtcaggaa
    30421 gagatttttt atcaattatt ttaaatgcat agtaagcatt gttttcaata tatggtccga
    30481 agatagtacc tacgggaagg ctagctgcag tatctttcag cataccttcg agctcggctt
    30541 tcttgacata tatcgaagtc atctgaccgt tgtttttagg tacaaatacg ctgtcaggtt
    30601 ctgtagaagt tttgtaagcg ttgatgattg cctctatttt acctcttaca tctgctgagt
    30661 cttttgcagt tggtttaaca tcgaatacca caaatgatac tcttctggtt tcctggtctg
    30721 acgtgtatgc acctttgttt tccttaaggt aagctgcata atcactgtct tcaagcttta
    30781 cttgatcatc attaatagtt tcgaaaggaa cctttacata agtgaggtca accatgtcgt
    30841 ctctttcctt ttggagtgct tcggccatga agtttggtgt ataaatacct ttgacaacaa
    30901 ggtttgagaa tttttcctga agtttttctt tgaatatttc tcgttctacc tctgcccatg
    30961 ctcttcttcc ttcttcaggt agtgtattgt tttcgatagc tttctttact tcattcagtt
    31021 gggttctgtc tattctgcca gtctgttggt cagcgaactg ctgttggatg acaggactta
    31081 agttgttgcc gaatgccaag tctttcattt catctgtgct tacgccaagc cctaatttat
    31141 cagcttcctt ggttaccaat actttgtcaa ccaaatattc ccataagaaa gatcttcgtg
    31201 cgtatggttc tgcggtcgaa tttttaaaca gagcttcttc aacgttttga aactccatat
    31261 aatctatgtt ttcgccagct acggttccaa gagtactgcc tgtgcctgct atgcttcggt
    31321 tgccttgaat atccattaga ataaagccaa aaacacctaa agcaatgagt gcaataatga
    31381 ggcttgagcg tttcctgatt tgagtaataa gtgccattta gttgctaatt tactgatttc
    31441 tgttaatagt attgcagtgt aatattttat atatagataa aaatagtata attttaatta
    31501 ttgcaccttg attaaaaatt tttgcaaaag taaactgttt cgtacttaaa atcaaaaaaa
    31561 tcttatggtg ttacggaaat gatcctccat tggtttcaat tctgatccat ttgtctgtca
    31621 ttatttcttg atataagcag caaatgcttc caattcgtta tagaattctt ctccatattt
    31681 tctgattatt ccttgcttaa taaatttatg gagcggtatc tgttcttttt ttcctttagc
    31741 acaggcggca ttgcaaatat gccaacgatg gtagctcagt atttcaaacc cggtatgtgg
    31801 atcgtgatct atacgcaccg ggtaaagatg gcaggaaacc ggttttaaaa aatctgattt
    31861 atcgtcataa tatgcttttt caatggcaca atatgctaca ccattttctc tttttaaaaa
    31921 cacacaatcg ccattggctt taaggcttgt acccaacgtg ttgatgtctt tataatatgt
    31981 tgaataccct tctgtatcaa tgatagaatt actctcttgg tcgaggtatt gttggatgga
    32041 agtaaggttg ctatcaatct ggtcgatttc atcgttttct atcggggcac cataatcgcc
    32101 ttcccagcaa caggcacctt tgcaggcatt gaggtcgcaa gcaaaataag actgcaggac
    32161 attatcgttt accagtttat cttgtattat cagcattggt ttggttaaat ttggatacaa
    32221 atgtgaaaaa tatttttttg aaagactaat ttttcgtttt tttgtgttct tatatatttt
    32281 aaacctatta gaaatatgaa aaaattattt atactgatgt ttatctgttc atttgcaagt
    32341 ggtctgtttg cccagtcaac caacagcacc agagaagaag caaaaagaac aaccgacata
    32401 ctgaaagcaa aatacaatct tgacgcaatg caggaaatgc aaatgcagaa aattcaggag
    32461 cgcagagtcg aaaatgcgcg cgaattcgaa tatttaaaga atacagatga aaacctgtat
    32521 tataagaaaa agaggtcaaa tatagaaggt acgaatgctt caataaaaag aatgctgact
    32581 tctgatcaaa taaaaattta tcagcaagac caactcgagt taagaaaaaa gcgtgcagaa
    32641 aagtcaaaag aactaaaatc taaaggcatt tccggctttg agctagaaaa agcactgata
    32701 gatattgaat agttgaaaaa aaaatatgaa atttgcgccc ggtttgagaa acattgtttt
    32761 acagaccggg tttattattt attactaaag agataaaatc gtgatacaag atccactaaa
    32821 aatgaagttt tttgagaaag ctcaaattgc ttcagaaaac cttaaaaaat tagcaaaaga
    32881 taatggtgac cttaaactcg acgaagtaac cgtctcgcaa atgataggcg gaatgcgggg
    32941 cgtgaagtca ctactttggg aaacatcaga actcgatgcc gaactcggaa taaaattccg
    33001 cggatacgat atcttccagc tacgtgagct gttaccaaaa ttcaacggag gcacagaacc
    33061 acttcctgaa tcattgttct ggcttatgct tgtagatgaa atacctactc aagctgaagc
    33121 actttggctt accgaccagt gggtgagaag aagcctgatt cccgaacacg ttttcaaggc
    33181 acttgatgca ttgcctactt caacacaccc gatgactcag ttcagcattg gtatttcggc
    33241 tctacagaca gaaagtattt ttgcaagaag gcatgcagaa ggtatgaata aaaacgaata
    33301 ctggaatgct acttatgaag ataccatgaa cctgttagcc aaattgccgc ggctggccgc
    33361 ttatatctac cgtagatcat ttcacaatgg caaacacatc agccctgata tcacattgga
    33421 ctgggcaggt aactttgcac acatgttggg catttcaaat cctgagttta aggagttgat
    33481 gcgactgtac ctaaccatac atggtgacca cgaaggcggt aatgcctcag cacatactac
    33541 ccatcttgta ggttcaacgc ttagtgattc ttacctttca ctggctggtg gtatgaatgc
    33601 acttgccggc ccacttcacg gtttggccaa ccaggaagta atttcatgga ttattaaaat
    33661 gatggatata cttggtactg agacaccttc agacaagcaa gtagaagaat acataaaggc
    33721 gaccttggct ggcggacgtg tagtacctgg ttatggtcat gccgtacttc gaaaaccaga
    33781 cccaagattc ttggtacaaa aacagtttgc agaagaatac ggactaaagt cgaatctgct
    33841 caatgtggtt tggcaattgt tcaggttagc accacctatg cttgaatcaa ccggtaaagt
    33901 taagaatcct tggcctaatg tcgatgcaca ttcgggtgcg tctttggttt attatggctt
    33961 gaaagagcac aacttctata cagtactgtt tggagtatca agatctctcg gtgtgttggc
    34021 ttcattatgt tggagcagag cattaagcct gggactcgaa cggccaaaat cagtatctac
    34081 aaagtggtta catacttttg ttgctaatca attggctaca aaatcgtaat aaaggagtct
    34141 aatgttttac aaatattatt caacattatt ctgatattta gttagctttg ctgagttaaa
    34201 aaattattaa ttatatcatc aattaaaaca taagatatga cttttccagc cgaactgaaa
    34261 tacacgcaag aacacgagtg gatacgtatt gaaggcgatt tcgctttcat aggtattaca
    34321 gaatttgcac aaagtgaatt gggcgatatc gtttatgtcg aggttgaaac cgtaggagag
    34381 gtcgtcaaca gcggagatgt attcggaaca atagaagctg taaaaacaac atcagatctg
    34441 ttcatgccga taactggaga agtcttggag ctcaatgaag caataagtga agcaggtggt
    34501 gatgatcctg ctttaatcaa ttcagaccct tatggtgaag gctggattat caaagtaaaa
    34561 atatcggata agtcagagat tgacggctta cttgatgctg atgcttatgc agcattggtc
    34621 aattaattac tttgcagctt aaaagttttc aaacaaataa attgatactg ttttggttac
    34681 caaatgggtt atttgtttgg ttctgtgata agacattgtt aaaatacaac ttttagcatg
    34741 gttgttgcgt tgtacgatga ataagtatgt cattctgaaa atgcgttatt tcatcatatt
    34801 tttcttttta ttggtttcat tagggctaca agctcaggaa gtaaaactcg ctcagcaata
    34861 ttacagcacc ggtgagtacg aaaaagctgc taccctttac caaaaattgc tttcgcagaa
    34921 cgaaaacaat gaatattatt tcaataagta catcgaatgt ctgatggcct tacaggattt
    34981 cgagtctggt gaaaaagcca tccgcaaaca aataaaaaaa tttcctaagg aagtctcata
    35041 ttatgtgagt cttggcaaat tgctcgaaag acaaaacaga aaagaagaag ctgaggcaca
    35101 atataccaaa gctgttgaag agcttcctgc agaccaaggc agcatcatca aacttgccaa
    35161 tgcttttgtt acacttgcca actacgataa agccatagaa acatacgaga aaggaagtaa
    35221 actgctcaac aacaagagca tcttttccta tagccttgca gatttgtatt tccgcaaggg
    35281 tgatacagcc aaaatgattc agtattatct gtatgccttg tcgcttgaca ccaatctgaa
    35341 ggtttcgctc caaacatttt ttcagaattc gtttaccgac aaagaatatg atgagctgca
    35401 ggcacagttg ctggaaaaaa tacaggaaaa tcctgataat cttgactatg ttgaaatgct
    35461 cgtatggcag tatatgcagc ggaagaacta cgcaagtgct ctgcgctata ccaaatccat
    35521 tgacaaaagg cttcagggca atggatcgaa aatataccag ctgggcagta ctgcactttt
    35581 gcaaaaagat tacgacgccg ctattgatgc atttacctac atcattacag agaaaggtaa
    35641 gattagccct tattatattg aagcaaagaa agaacaactt aaagcaagaa ggttaaaaat
    35701 aacggataca tataattaca cccaggaaga tctgaaacag atagaggcag actatgaaaa
    35761 ttttattgaa acttcgggta aaaattttca gacaagtcag attatgctag aatgggctac
    35821 aatggaagga ttatatctca ataatctgcc aaaggctatc agcatattga atgaaatgat
    35881 taatctgcag ggcatcaaca ggaacattca ggcagaagct aagctacaac taggcgattt
    35941 ttatctcatg acaggtgaag tgtgggaagc aaccttgctc tattcgcagg tagataaaga
    36001 ttttggtgaa gatgtactag ggcacgaagc cagattccgg aatgcaaaat tatcatatta
    36061 caatggtgat tttgactggg ctcaggccca attcgatgtg ttgaaagcat cgacttcaaa
    36121 actcatttca aatgatgcaa tagacttgtc ggtctttatc cttgacaatg ttggtcttga
    36181 ttctattacc aaaccgctcg ccatgtatgc ccaagctgag cttctcgtat ttcagaataa
    36241 gatagatgag gcattcagca aacttgattc tattagtact aattatccag aacatggctt
    36301 gctcgatgat attgcctatt tgaaagcaaa gatttatacc aagcgaaaag attataccaa
    36361 ggctatagaa atgtacagtt atgtggtaga aaaattccct gaagacataa gggcagacaa
    36421 tgcactgttc gaaatggccg atctgtatga aaatgcactc aatgataagg aaaaagccaa
    36481 ggaattgtac gaaaaactct ttatgaatta ttcgaacagc atactcgcta ctgatgctcg
    36541 taagcgcttc agacaattga ggggtgataa aatatttcaa taaaccaatc aaaacttaag
    36601 ctattaaata aatgtacatc tataacgtaa cgttaaaagt ggacccgtcg atagaagaag
    36661 actggcttat ctggatgaaa gacatccaca taccggaggt gttgagttgc gggatattca
    36721 aggactataa aatgctgttg cttaccaatt ctcctttcta tgacgaatca cctggcagta
    36781 cttttatagt gcagtatttt tttgacaata tggatgagta tgaaagatat caggccgaat
    36841 atgctccgac ataccaacgc gaacacatac agcgctatgg cgagaaagtt cttgcattca
    36901 gaacagtgat gcgttatctt tgatgtcagg actacttacg tagttcgggt tttaggcggt
    36961 aggcttcctc cagtatttca tttccttttt ttgggtttcc tccatcacgg taagcagaac
    37021 ccataaaaaa caagatatct gcattattgg ggtcatactc caacgccttt tcaaactctt
    37081 tgatggcatt tgggaagtca cggttgatag ccatcaagca gccaaggttt ctacgagcat
    37141 ctacaaatct cgggtcg
//
LOCUS       DAOQQY010000004        36912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_320_length_36912_cov_6.958542, whole genome shotgun sequence.
ACCESSION   DAOQQY010000004 DAOQQY010000000
VERSION     DAOQQY010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 36912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36912
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_320_length_36912_cov_6.958542"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..1382)
                     /locus_tag="PLE23_00505"
     CDS             complement(27..1382)
                     /locus_tag="PLE23_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81773.1"
                     /translation="MTNKILFLAILLFPVMLYCQSADNSPYSRFGFGNIVNSQKVSSV
                     GMGRLSSVYGDSYNTNFDNPATLTSLINATYEGGIYAKYTQIEQQTSSQTQWSGNMSY
                     LSISFPLKNQISESLQKKPSNYKWVMGFNLSPYSTNNYNLQETRNVDSIGEVQTLYNG
                     TGGSYRMVWGNAVKYKNLSVGLNIGWLFGKSSYGRQILFNDLAYSYSDVFTDEYSIRG
                     FSWKAGATYRFDLSQTNDKKEAKYPGRNLLIGVSGNSAQSFNTTQSFLYRRVRTGIQS
                     PDGSTIDTFKISPAEGVKGKGKLPSELNVGVLYTHSPKLKFGVDYSFSDWSRYENDAK
                     PNEQILKLDKQYKIAAGVEYSPNPDDYKYYFKRIRYRLGAYYQHDPRLINGSELTETG
                     LSFGMGMPIRLPRQQIAFVHWALEAGKFGNVNVINEKYLRLHFAFNFSDTSWFYKRKY
                     D"
     gene            complement(1372..2103)
                     /locus_tag="PLE23_00510"
     CDS             complement(1372..2103)
                     /locus_tag="PLE23_00510"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HMX81774.1"
                     /translation="MQLAIDIGNTYIKAGLFSEDKLLGTIRFVAGEEMDFLNFIAGKK
                     INYTIFSSVESSEAEAVIGLLKDTKIIQLDHNTPLPLINNYLSPETLGKDRLAGALGA
                     WVMLGGKSVLVIDAGTCIKYDFVDESQGYMGGSISPGLQMRLDAMHHFTARLPSFTLK
                     RTTDFYGNDTFSAMMTGVVTGAVGECSHFIQVFKKRFGKFNVILTGGNADFLSLQLEN
                     KYSVEPNLILTGLNSILNFNKNNDK"
     gene            complement(2110..3645)
                     /gene="purH"
                     /locus_tag="PLE23_00515"
     CDS             complement(2110..3645)
                     /gene="purH"
                     /locus_tag="PLE23_00515"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMX81775.1"
                     /translation="MSQLRSIKIRSALISVYSKEGLAPLVQEMHRLGIDIISTGGTQS
                     FIESLNIPVTPVEKYTQYPSILDGRVKTLHPQIFGGILARREREHLVQLETYDIPEID
                     MVIVDLYPFEKTVASTDDDEAIIEKIDIGGISLIRAAAKNFRDVVVIPSVEQYSDAMK
                     IMQQQEGVTTYDERHSLARKAFAVSSHYDTAIYKYFNKTAHEPSFHQAIDIADELRYG
                     ENPHQKAYFYGKMNDVIEKLHGKELSYNNLVDVDSAINLIQDFRLSQPTIAILKHTNP
                     CGVATREKLIDAWNAALAGDPISAFGGVIISNVAVDVETAENINTMFYEVLIAPDFSN
                     KAIEVLTEKKNRILLKLKDTPLPQKSFKSILNGVLEQDADNHVHLSKDWTLATTRQPE
                     ASELIDMEFANKCVKHLKSNAIVLVKNQMMLGMGCGQTSRVEALNQAIQKAKHFGFSL
                     TGAVMGSDAFFPFPDCVEIAHKQGISAVVQPGGSVKDQDSTDYCNNNGMTMMITGYRH
                     FKH"
     gene            3878..5599
                     /gene="dnaX"
                     /locus_tag="PLE23_00520"
     CDS             3878..5599
                     /gene="dnaX"
                     /locus_tag="PLE23_00520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMX81776.1"
                     /translation="MSQFIVSARKYRPTRFNDVIGQEHVSVTLRNALQTGHLAHAFLF
                     TGPRGVGKTTCARILARVINCENPTKEYDACNECASCKAFNENASFNILELDAASNNG
                     VDQMRALTEQVRFQPQQGKYKVFIIDEVHMLTTQAFNAFLKTLEEPPPHAIFILATTE
                     KHKIIPTILSRCQIYDFKRIQIRDMVGKLQEICKEEGIDAEEKALHIIAQKADGAMRD
                     ALSIFDRIVSFSGKKITYNDVINNLNVLDYVYYFRASDCMMTEDAGGLLMLLDEISNK
                     GFEEEIFMSGLADHLRNLLICKDQRTVALLELSEDFTKKYIETAPLFAEIDLLNALAV
                     INHYDIHTRTVRNKKLHTELHLLRLANIRHLYKVDTSTTEVSQEKKTPELSQPKIQLS
                     NNQHVIIKASPEPAIVGNPAPAVPKSDTKPVLDTPTFFSLEALEQSIMEEDNDHTDAL
                     ALNEENLRTCWIDYINGHQSPSVKAILLNTLTRIENNTIVATVGTTLAKEMILQEKNI
                     TQHLRSRFGLPNLMIEIEIDTSLTPEQAPAKPKPLTGKEKYEKLVAINPDVDELRKKL
                     NLRIDEL"
     gene            5615..6673
                     /locus_tag="PLE23_00525"
     CDS             5615..6673
                     /locus_tag="PLE23_00525"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HMX81777.1"
                     /translation="MFYQYFIKPVFFCFNPETAHAMAMRALKLLCAIPGGKLLTNKLF
                     NYQNKTLEISAFGLNFPNPVGLAAGFDKDGKYFSVLENLGFGFIEIGTVTPRPQDGNP
                     KPRLFRLPGDEALINRMGFNNEGLEVLKNRLKNRTGKVIIGGNIGKNKDTPNELAIND
                     YLEGFHQLFDVVDYFVVNVSSPNTPNLRALQEKEPLTKILSQLQESNLKKPSPKAILL
                     KIAPDLTNEQLDDIIEIVKTTRISGVIATNTTITRNNLTTNNTQIESIGNGGLSGKPV
                     REMSTRVIRYIHEHSGGTIPVIGVGGIHTAQDAKEKLDAGACLVQVFTGFIYEGPSIV
                     RRINKYLAKSCGEGSHSL"
     gene            6736..8577
                     /gene="uvrC"
                     /locus_tag="PLE23_00530"
     CDS             6736..8577
                     /gene="uvrC"
                     /locus_tag="PLE23_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMX81778.1"
                     /translation="MTQDDFRNISSTLPSDPGIYKYFNEEDTILYVGKAKNLKKRLAS
                     YFVSSKNLPYKTRLMLRHATRIEYTITKSESDALLLENSLIKKFQPRYNVMLKDAKSY
                     SYICISNEHFPKVFFTRRIIRNGSLYFGPYTSKFRVNILLDLIKSLFPLRNCNLQLTP
                     DNIAKRKFNVCLEYHIKNCEGPCIGAESEEHYNKKIEQIKNILKGHLKIVKDYLLDEM
                     NRYATNLDFEKAQQMKDKLIAFEDYQAKSTVVNINIRDVDVFYIESNDTTAFVNYLKI
                     IDGSIVNTHTLELIKNLDDDDNDLLSYSIDVLREKFNSIAPEIIVPFEPIVSDTSLLV
                     TIPKVGDKKKLLEMAEKNVKYFILQQQQAAINSLRKLPATERILKTLQADLRMSAMPL
                     HIECFDNSNIQGTNPVASCVVYKNAKPSKADYRHFKIKTVVGPDDFASMYEIVTRRYA
                     RMLAENKTLPQLVVIDGGKGQLSSAVSALKDLGILEKFTVIGIAKKLEEIFFPEDSIP
                     LYINKKSESLKLIQQIRNEAHRFAITFHRDQRSKNFTKTVLTDIQGVGPKTADKLLKH
                     FGSFKKIMDASDEELKTVAGVNVARLIKEYYTAEDVAEDKDEVTNED"
     gene            complement(8594..10027)
                     /locus_tag="PLE23_00535"
     CDS             complement(8594..10027)
                     /locus_tag="PLE23_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81779.1"
                     /translation="MSDRTRLKKFSEFAVSLLPHETSYLLSVQQFQDDERLRMLETIH
                     QVAVNPSKTDLFDVGIDKRKYSHLKNWIEERLADVNVDFQFDWITTTLKHILTDSLTT
                     DDERKILKLIRSGETNMFYFTKFFELLAEYRQFLLIRLRYQEHNTIDQYITTHQVLYD
                     RNRDVFDKLHNATQDIINHYSSITNESIHWETWLTEVFYDESLEGYIRYMALVRLTFV
                     HFNYRDFEGLVEKYNAIDKWFVKGKFYSKRILLNYYSNRVLLHARFKEYDKAVEYGYL
                     SIRQKNNDYIHYVNNLCAILLRTNRNKEALQLMRDAYPEMRLTPSFHNKIGFISFYMR
                     CLMNNQQYKNAENYAETFLKAYRQEIFKYRWHIFFTAYLEAILNQNKYEKIIKVVRNF
                     NLLEKDKSYQNKATYLPTISWYYAISACKEGLMDRNQLSETIYSYVRSLESSPERVNH
                     LETMVAGIKKHIPGILPNIELKRLLEE"
     gene            complement(10086..11087)
                     /gene="obgE"
                     /locus_tag="PLE23_00540"
     CDS             complement(10086..11087)
                     /gene="obgE"
                     /locus_tag="PLE23_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMX81780.1"
                     /translation="MAEQNFVDYVKICCRSGAGGAGSKHFASDPINPRGGPDGGDGGR
                     GGHIIVKGNKQMWTLLPLKYRKHVIAGDGGNGAKNNRFGKYGEDIVLEVPIGTIVRDV
                     ESGEITAEINEDGQEIIIAKGGRGGLGNVHFKSATNQAPQYAQPGEPGVEEWKILELK
                     VLADVGLVGFPNAGKSTLLAAITAAKPKIDSYPFTTIVPNLGIVPYHGHMSFIMADIP
                     GIIEDAHLGKGLGHRFLKHIERNAVLLFMVPADTDHIGNEYNVLLHELEMFNEELLDK
                     PRILAVTKSDMIDDELKEMLKSDLPPGIKTVFISSVTNKGLKELKDMLWQELNPVQE"
     gene            complement(11123..11695)
                     /locus_tag="PLE23_00545"
     CDS             complement(11123..11695)
                     /locus_tag="PLE23_00545"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMX81781.1"
                     /translation="MLNIILFGPPGSGKGTQAAKLVEKYHLLHISTGDLFRYEMGNNT
                     PLGLLAKEYMSKGQLVPDEVTIGMLKNKLEAHPEAQGHIFDGFPRTITQAQALDQLLT
                     EKNQTINALVALDVDDEEIVKRILLRGQTSGRPDDNDESIVKNRINVYNDETKPVFDY
                     YTTSGKAFKVHGIGTVEEIFNRLCESIDSI"
     gene            complement(11788..12342)
                     /locus_tag="PLE23_00550"
     CDS             complement(11788..12342)
                     /locus_tag="PLE23_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HMX81782.1"
                     /translation="MRVKNIKLQILLLAIVLVSCSAFREAPLKALDYPARATEITGGG
                     PSGSGTLTEEQIRQEVVSLGKQFEGMRYRSGGKEPATGFDCSGFTGFLFRNFDINLSA
                     SSGTQILDGKAKRIKEVEPGDLIFFSRTDGGRIFHVAMVISNTDDGITVIHSTNSNGI
                     ILTNIMKDEYWFPKIAGARDVISE"
     gene            complement(12510..13271)
                     /locus_tag="PLE23_00555"
     CDS             complement(12510..13271)
                     /locus_tag="PLE23_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMX81783.1"
                     /translation="MRLTLLQTDLVWQDTDQNLKNIEAKIEKLHGKTDLIVLPEMFNT
                     GFTMNTGHAKEADRVIFWLKEQAAKLETAIIGSIIVNEEEKYYNRLLLVTNEGVIHHY
                     DKRHLFTFSHEDKYYKPGQKRLEYELKGWRICPLICYDLRFPVWSRNTNNYDLLIYVA
                     NWPSVRSFAWKTLLAARAIENQSFVAGVNRIGTDGKGHEYSGDSVCLDFLGNTMVSCE
                     DREEVLTIELGKSTLTKFRHAFPALADKDNFNLIL"
     gene            complement(13396..13965)
                     /locus_tag="PLE23_00560"
     CDS             complement(13396..13965)
                     /locus_tag="PLE23_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HMX81784.1"
                     /translation="MEKVAIKANTRAEVGSTSAKSARNNGLVPGVIYGGKDVVHFEAD
                     PASFRHVVYSPDFKILEVEIGGKTHKCILKSTQFHPVSEKLEHIDLLELIEGQTLKCK
                     IPLKFTGASPGVKVGGKFVQKLREVNVKTTPDKLVGELFADISSMELGSTIRIRDIKA
                     QDGITIENPPAIPIASIDIPRALRNQGKS"
     gene            complement(14071..15000)
                     /locus_tag="PLE23_00565"
     CDS             complement(14071..15000)
                     /locus_tag="PLE23_00565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMX81785.1"
                     /translation="MEVKLFSGTLSQPLSEKVADYYGYPLSDCLLQKFSDGEMQPVIN
                     ESVRGAYVFFIQSTFAPADNLLELLLFIDAAKRASAGYITAVIPYFGYARQDRKDKAR
                     VPISARLMANLIETAGANRIMTMDLHADQIQGFFDIPVDHLKSEAIFIPAIEQLTLPD
                     IVFASPDVGGVKRARRYAKYFAREMVICDKYRKRANEVASMTVIGDVKGKDVILVDDI
                     IDTAGTLCKAADMLIEQGASSVRAFITHPVLSGDAYKNIEKSKIEEIYVTDTIPLKQS
                     SPKIKVLSSAQLFARAIRNTHEHRSISALFIEK"
     gene            15368..15844
                     /locus_tag="PLE23_00570"
     CDS             15368..15844
                     /locus_tag="PLE23_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81786.1"
                     /translation="MVVLFFTQEKHTPMDGNTFMGQKLMAVVFGMVAAVICIVAFDSI
                     NLMVYPPPKNFSYENTEMAKAYLFNAPGYAKLIIILGLAFAGFIGGMVSAKFDKSSDK
                     RTAWIVGGLLFVQYVINFSYIPYPVVIIVAGLALVVPATYLGYFVFVKYLKVKVTK"
     gene            complement(15847..17097)
                     /locus_tag="PLE23_00575"
     CDS             complement(15847..17097)
                     /locus_tag="PLE23_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMX81787.1"
                     /translation="MEPFSIELLGSLIDSVTEGILVCSDESKIIYVNKAVENLFGYSS
                     ESLLGQNLFVLIPERYRPKHNLYVTDFFNQPANRRMGVGRDLSGLKSNGEEFPVEISL
                     SYFRHGNSYLVFAFIVDITQRKNIEQQLIEKNRSLEKLTEEMTVLTTELESKVFERTM
                     ILREALDELEKSQKELSDSLNKEKELNEIKSRFVSMASHEFRTPLSTILSSASLVAKY
                     RIEEDQPNRERHVNKIKDAVKHLNELLEDFLSIGKLEEGKVGIKVETFDISSFVYEII
                     EEIKTILKPGQTIKYVLTGAGSFTTDKRILKNILLNVLSNATKFSYENSPIKLIIDSN
                     NKLQIQIIDQGIGIAQEDISHLFSNFYRGKNATNIQGTGLGLPIIKRYLDMIKGNVTV
                     ESELNKGSTFIISLAKMHAQDTVE"
     gene            complement(17131..19752)
                     /locus_tag="PLE23_00580"
     CDS             complement(17131..19752)
                     /locus_tag="PLE23_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HMX81788.1"
                     /translation="MSQQYDPSKDLETQKEQIISVKGMYKSYFLDYASYVILERAVPA
                     LEDGLKPVQRRILHALREMDDGRYNKVANIIGQTMQYHPHGDAAIGDAMVNLGQKDLL
                     IDCQGNWGDIRTGDGAAAPRYIEARLTKFALEVAFNKQTTEWQLSYDGRKNEPVVLPM
                     KFPLLLAQGVEGIAVGLSTKIMPHNFIELIDASIAYLQNEPFQLYPDFLTGGSVDVAD
                     YNDGQRGGKIRVRAKIDASDKKHLVIRELPYGVTTTALIESIVKAADKGKIKIKKISD
                     NTAAEVEILIELLPGVSPDVTMDALYAFTACEVSISPNACVILDDKPHFVTVSDMLKN
                     ATEFSKKLLGWELDIKKNELEESWLFASLEKIFIENRIYHDIEECETWEEVMTTIEAG
                     LKKYVATPSDYNGKTDRRLKLLRELTTEDYTRLTEIKIKRITKFNSFKADEHIRQLEA
                     DLEETKYNIAHLNDFTINYYQRLKEKYGKGRERKTEIKNFESIIAQEVVANNAKLYAN
                     YKEGFVGFGLKKDEFITDCSDIDDVIAFRKDGKFSVVRIADKVFVGKDLIYTNVWKKN
                     DERTTYNLIYLDGRSGKTFAKRFNVTSITRDKEYDLTQGTPNSKVIYFSANPNGESET
                     VTISLSATSSARIKQFEFDFGELAIKGRNSQGNTITKYPVRKVIQKELGKSTLGAKVF
                     WFDETTGKLNTDERGRKIGAFDTGDMILSIYRDGSYLISEPDTTTRYEVEKLIDLNKF
                     DPDRILNVLYYEAEKGWSMIKRFKIEATSTGQRYRFITDHKHSKLYYVTLTPAVRLTC
                     VWDSGKKINEQEIELDDFVTVKGWKSIGNKVCDYPLLEADIKITEPTPMQEKLHPGDQ
                     IDFDIPPQKGQGKLFDE"
     gene            20004..20891
                     /locus_tag="PLE23_00585"
     CDS             20004..20891
                     /locus_tag="PLE23_00585"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMX81789.1"
                     /translation="MLKFISKLFNILLFRILLAISRLPFSVLYTLSSFLFFLLYYILK
                     YRRNIVRQNLLGSLPSTINPVVIEKNFYTNLCEMFLESLKCMTISEHNILQTIHCDNP
                     ELANKYFHSGKSIILMSGHFFNWEILIYSMNLLFHHQAIGVGKKLTNQEFNRLTNERR
                     SRFGLNIIHADNIREEFATLANANTATLFLSDQYPGGATKGYFKKFLNKPSYFLYGAE
                     KYARQYDLPVLYARFEKLKQGSYKIHLTEITDKPNDTDYGFIIEEYVRLLEQNILEQP
                     EIWLWSHKRWKNIENYYHN"
     gene            21005..24049
                     /locus_tag="PLE23_00590"
     CDS             21005..24049
                     /locus_tag="PLE23_00590"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMX81790.1"
                     /translation="MKSKLFLTLISLAICLHPFAQTSYFQQQVDYKIDVTLDDEHHLL
                     QGWIEMEYTNNSTQTLDKIYMHLWPNAYKNDKSAFAKQQLKNNKRSFHFSEKKDRGYL
                     SGLNFTVNGTTTVIQEYEGNPDIVLLTLPQPLAPHQKISIKTPFTLKIPKVFSRLGHT
                     GQNYFITQWYPKPAVFDTKGWHPIPYLDQGEFYSEFGNFDVKITLPANYVVASTGTTT
                     DDTEFFNNRVSLTTKLLANADAAGNWKASSSEGSKTLHYHAENVHDFAWFASKEFMIV
                     KDTARLEEGNQLPVYGYFLLKNKKNWARSAFYVKRAVEYRSRRIGDYPWPHASAVDGD
                     LIAGGGMEYPMVTVISAGGSAQSLDNVIEHEVGHNWFYGILASNERDDAWMDEGINSY
                     YEADYMTEYYKPKNEAHVQKKRPRGFSLDESLISSIFMKRKLDQPLSLTSAEFINVNY
                     GLDVYKKTARYFKILELYLGTKSFDAAMKEYYRQWSFKHPYPDDLKQVLEKSTGKNLS
                     WLFDDLIQKNMPVDYKIVKASNNSVTIKNIAGNPTPLNLTVTNKNGSSATYFYEGFKD
                     VKSIDQDMAEVKSLTIDADNYLMDASTSNNYYHTSGICKASRPWTPKIFGLIEQKDKN
                     VTNILPAIGWNLYDKFMAGILISNPILPGRNFRYFVAPMIATSDGSVVGQGMLEQNFY
                     SYKNPERLAIGISGRRYAFNFIDNSLLKERLHYIKVTPYITAEFNRWHTWAHKLTYKT
                     NILVKDQVNIKQVDGAVKAEVKADNQYIHTLDYTLTTHNGLSPMEANIRLEQQNYNST
                     KGKEHYLKLSLEYNTKLYYNTKRSVDIRIFAGGYLDNTAKNSGATNSDYFARGSMSLA
                     STAADDYLFECPYLGRSADQGFFSRQIDISDGGFKFPLAYKYGQFGYTNSFLAALNLK
                     AGLPVDMPLKLPIKPYFDLGYYNDLRPINSDKNWKDNVVWAGGFMLDFGSYVGIYFPV
                     VQSKSLSDQYNQSVGANYLSRISFSIKLPQLRISELVKSFSF"
     gene            complement(24090..24788)
                     /locus_tag="PLE23_00595"
     CDS             complement(24090..24788)
                     /locus_tag="PLE23_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMX81791.1"
                     /translation="MFIYLLPLLTISCRQRQYHHTAIDQTASSVHSADTISRAVAELP
                     THKQLPVPAGFSEIAPTDTSFILDLRYASDNNFVKTRLYDCPRCILKTEVADRLRKAN
                     ELFRKKKLKLVLLDCYRPHSVQEKLWNAKPDPHFVMRPWKGSLHTRAIAVDVTLADQD
                     GNYLDMGSEFDEFSKKSYYLSKEINDNARTNRYLLCSIMRQAGFTGIRTEWWHFQYGL
                     AANNQLQKYEWECD"
     gene            complement(24802..26160)
                     /locus_tag="PLE23_00600"
     CDS             complement(24802..26160)
                     /locus_tag="PLE23_00600"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMX81792.1"
                     /translation="MRHFFTLLLTIGSLLAYGQSKNITLSCDLKNCGADSINLYEFNG
                     MSFDMVKAAKGENRVAKFELNKSTTPRMYYVGFSISDLRPVILGTEDNVNINVVCEAV
                     KKSDIPGSPINKAYDGLLMKLSEIRFQENQIFSTYTSSQRTEKNEADFKKSLADLDKR
                     KISLKDSLMKINAFLGNTAAINTYLSFANNKGKYTDEIDYFANEFFKQSKLDDKNLEQ
                     NPWVTEIFKAYTQTLQRVGRGNTYIINSIRTQLKKIPQTSNTYRLALGGVVSGIEQNT
                     DVLFVEFADEFSTKYAKTNSMDIGLLKPRLDAIKQLMIGLPAPDFTLETPDGKKVSLK
                     DFKGKVVLIDFWASWCGPCRRENPNVVAMYNRYKDKGFEILGVSLDRSKEPWIKAIAD
                     DKLTWPHVSDLKGWGSSAAALYGITSIPHTVLVDRDGRIVARQLRGEVLGKKLEEILG
                     GK"
     gene            complement(26174..28300)
                     /gene="ligA"
                     /locus_tag="PLE23_00605"
     CDS             complement(26174..28300)
                     /gene="ligA"
                     /locus_tag="PLE23_00605"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HMX81793.1"
                     /translation="MYSPEQQQTLTELTKKILSDAEYGIHQVPVQFAEQLRELIRFHE
                     WRYYIMNDPLISDYEYDVLYKKLQETEQEHPDLIIADSPTQRISPDISTGQETVSHLT
                     PMLSLANSYDLNDLIEFDNQIRKLAGIDPAKKIEYCVEPKYDGGSIAIVFENDSLIRA
                     ATRGDGQNGEDISNNILALKSVPRKVAFSDHNIVKAELRGEALIRKDQFEKINAEREE
                     EGLSRFANPRNAATGGLRTKDPRETADRKIEVFVYQLGFAVDSNHNDVLQTIPTHDEG
                     IELLGQLGFKIPVNERKVCHGIHEVEEFCQLWQKQRDSFDYEIDGMVIKVNDRKIQEL
                     CGYTSHHPRWAIAFKFQAKQATSRLEHVEFQVGKMGSVTPVAKVTPVSLAGVTVSSIS
                     LHNEEFIQSRDIRLGDMVLLERAGDVIPYIVKSLADVRKGDEVPIIFPENCPSCNTRL
                     VRVEGEAAWRCINEKCHEKIIQRLIFHASKDAMDIEGLGESLIRKFYDLGWIKDMADI
                     YTLDYEDISKLDGFGKKSAENLRKAIETAKKNPIHRFLHSLSIHHLGKKASKLLAGQI
                     DNVMDLKEWPIERYLAIKDIGPKVAKNTRTFFTNSENVELLERIQSAGVNFLSTNEDK
                     PTIGREDTLLSGKTILFTGTLSTMGRKEAEKLAENAGAKNLGAVSSNLNILVTGENAG
                     SKLEKAKKLGTVEILTEQQFLELINN"
     gene            complement(28328..29263)
                     /locus_tag="PLE23_00610"
     CDS             complement(28328..29263)
                     /locus_tag="PLE23_00610"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HMX81794.1"
                     /translation="MERLIFKYTQGLPGPLLICLAGIHGNEIAGIRALQRLEEMLVQE
                     TASKPHFVFTGTVIGLVGNVEASESQTRFIESDLNRMFLDESVTRILEANFSDLEAEE
                     LELRELLTTIDEFIKETLPTKVVILDLHTTSARGGLFAIPSDQEESIRIAKALHAPVI
                     KGFMSGLKGTTLHYFSNNGYKYDQPVIPLVFEAGQHTNPLSVNRMIAAVVNCLRTIGC
                     VDHDDVESQHDEILKAYSEHLPALTRLIHRHQIIRADEFVMKPGFENFQKIKKGELLA
                     RDKNGNIYSDYNGYILMPLYQRKGEDGFFIIQDDK"
     gene            complement(29251..29865)
                     /locus_tag="PLE23_00615"
     CDS             complement(29251..29865)
                     /locus_tag="PLE23_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HMX81795.1"
                     /translation="MDIEVIKGHLKQDSVLQPIVEKIVLPPLIVQQNVFKELTESIIS
                     QQLSVKVAAIITQRLHDLLGTVDPSPEDILRLSTEEKRAIGLSYQKAGYMDNIAGFWI
                     ENSLASTSWQSMSDDEILSLLTQIKGVGKWTVEMVLIFSLLRPNVFPYDDLGIKQQVI
                     KLYGLESTGKQLIKDMNEVAERWKPYRSFASRYIWKSKDLAWKD"
     gene            29952..31721
                     /locus_tag="PLE23_00620"
     CDS             29952..31721
                     /locus_tag="PLE23_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMX81796.1"
                     /translation="MKKILLFLFFSLALSSIIFGQSGWKTKLAPKVLTEYVEDNELDL
                     IVVFKDKADVSVAKYLSTKSAKGRYVYEALKATTQSSQKNVMAWAAYHHYKTKSFYIT
                     NALQIKIPAASLEQLASFVEVKYISNNGLLRYHEPVEKYPALLRGNEGKEWGIDTINA
                     NDVWALGYTGQGVVVGGEDTGYDWKHPAIQPKYRGWDGTTADHNYNWHDAIHELNPLN
                     GDSTSNPLNNPCGLDSPEPCDDGSHGTHTMGTMVGSTPGDTIGVAPDARWIGVRNMER
                     GWGKPSTYIEGFEWFLAPTDLNNQNPDPDKSPDVINNSWGCPPNEGCDLSNFGVMQDV
                     VANLKAAGIVVVVSAGNAGSGCGSIDDPAAIFEESFSIGATRSNDTIANFSSRGGVSI
                     DGSFRTKPNVSAPGVGVRSCTPGGNYQFFSGTSMAGPHVAGAVALILSAAPELKGQVD
                     VIEDILEQTAIPKYTDQECDSIPGSIVPNNTYGYGRIDVLQAVKLALKTVAANDIEKP
                     KTTVTSFPNPVSNTLYFDVKEFNGDILIEIFDVPGKLIYSSKQQVAGDKLVKCDFQHE
                     STGLYFYKITGQQLCVAGKFVKN"
     gene            31795..32835
                     /locus_tag="PLE23_00625"
     CDS             31795..32835
                     /locus_tag="PLE23_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HMX81797.1"
                     /translation="MRLYNRINGMELKRRMQDSTETRTTISFYKYYHIKNPRLFRDHF
                     FILLDKLDVLGRIYVAAEGVNGQMSVPTANIEKLREAFDTIDFFKGIRFNYAIDDNGK
                     SFFKLAIKLRDKILADGLNDKSFDVTQKGTHVDAAEFNALADDPNTVIIDMRNHYETE
                     VGHFKGAITPDVETFRESLPIIEEMLQNHKEQNLLMYCTGGIRCEKASAYFRYKGFKN
                     VFQLNGGIIEYARQVQEQKLENKFIGKNFVFDERLGERISPEIIASCHQCGKPCDAHT
                     NCANDACHLLFIQCDECAAKYSNCCSTKCCEFNQLGDDKKILRKTEVFNGTRFGKGRY
                     KAFNKDHSLDMS"
     gene            32909..33505
                     /gene="scpB"
                     /locus_tag="PLE23_00630"
     CDS             32909..33505
                     /gene="scpB"
                     /locus_tag="PLE23_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMX81798.1"
                     /translation="MAKLITDKPLAQHIESLIFSSEQPLAFDEIHQCLEQALELTLSK
                     NEIKKTIDALVERYQAPEFGIEIVEINDGYQFLTKGAYHHTIATLLKQYNKKRLSVAA
                     METLAIIAYKQPVSKTELEKIRGVNCDYSVHKLLEKELITISGRSDAPGKPILYATSE
                     KFMDYFGLKSIADLPKLKDFQSPENEIGFQQDIEIPAK"
     gene            33582..34118
                     /locus_tag="PLE23_00635"
     CDS             33582..34118
                     /locus_tag="PLE23_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2480 family protein"
                     /protein_id="HMX81799.1"
                     /translation="MENTQNKLVNRVAESGLITLNLEDYFPDTTFAHFDIKDYLFMEL
                     ILKEKDFREAMKQYDWASLEGKTLLLYCSNDAIIPTWAYMLVTTNAQAYVNDIFQGSE
                     QEYYRYKMKKIIEDIDFSKFDKKRIVVKGCGKKPVPVAAYIEITRGLKPYAQSIMYGE
                     PCSTVQIFKRPREINKPE"
     gene            34131..35033
                     /locus_tag="PLE23_00640"
     CDS             34131..35033
                     /locus_tag="PLE23_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMX81800.1"
                     /translation="MQDKKIYVYGSLVFLAFLLGINMQNTTRGTLPKGQYSFTDVKTI
                     DLNKDYNFAGESVPKDNFDALERLERELLVNAYWHSNTLMMVKKAKRYFPAIEKILKE
                     QGVPDDIKYLAAAESSFNNATSPSGAKGIWQFMESTGKEYGMEINNEVDERNHFEKST
                     IAACKMLTRLKDKFGSWTLAAAAYNMGGNGLAREMDNQRASTYYDLNLSNETLRYVLR
                     LIAIKEIFEDPARYGFATDDGPLYKPLDNYYELSVDTTISNLGDFANKYNTSYRMLKV
                     YNPWLVSYSLTNKDKKQYLIRIPK"
     gene            complement(35080..35268)
                     /locus_tag="PLE23_00645"
     CDS             complement(35080..35268)
                     /locus_tag="PLE23_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81801.1"
                     /translation="MKSKLLLLNKSLLVVAILIVTGIVACSRQKNISKDKITEAPSHN
                     APDQDKIDSIKASYNKKK"
     gene            complement(35240..>36912)
                     /locus_tag="PLE23_00650"
     CDS             complement(35240..>36912)
                     /locus_tag="PLE23_00650"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81802.1"
                     /translation="SDPEVSKIKEYLTQNKKKTIAAQTENARTAEDPEILLKFEGPWM
                     VKGTPPDLSFAVSTTGNIVALNNDGIEYYNTAGSYLGGEYWSDFYSASELSSKKYDPR
                     ILFDSESNRFVMVLLHGSKPDVSKVLLAISYSDNPKEDGWYYYTIKGDLTNSNLWFDY
                     PNLGMSANDIFITGNLYDSAGKFSRVMLMQIDKSSVYAKDPIKYFYYTNLSSEPFGAF
                     TLFPLSYGLKGNYGPGMYLVSNRSSGSDKLRLWYIDKPYSSGNPTLYSYPITVKEYSV
                     GGNAGQYGTSELLDVGDCRILSGFYLNGTIHVVHNTSIDNGWSAIDYHRINVKSQTAE
                     STDFGLSGSYDYAYPAVGSYANNPNDKSVIIAFLRSGDDIYPQCRVVHVDNNMDWSGS
                     TLVQAGDTYVDLVTSTTERWGDYITVCRQFVNNAQYPVLWMTGMYGGNVSSPSTKNTF
                     RSKIAKIFALKTASNEPTLTEAPTKTYPVPIKDIFNHEFQLADAQWITIELVDLEGKM
                     IRELFKDFLKAGKHKLSFTVNNLTAGTYILTILGDNNYKNEVKVVVVE"
BASE COUNT        10854 a         7765 c         6980 g        11313 t
ORIGIN      
        1 aattcacctg ctcaggtaaa taaattctaa tcgtattttc gtttgtaaaa ccagctggta
       61 tcgctaaaat taaatgcgaa gtgtagtctg agatattttt cgttgataac attgacatta
      121 ccaaacttgc ctgcttcaag tgcccaatgt acaaatgcta tctgttggcg aggcagcctt
      181 ataggcatac ccataccaaa gcttaggcct gtttctgtca gttcagatcc attaatcaac
      241 ctcggatcat gctggtaata tgctcccagt ctgtatctga tacgtttgaa ataatatttg
      301 taatcatccg gattcggtga atattctaca ccggctgcta ttttatactg cttgtcaagc
      361 ttgagaatct gttcattagg ttttgcatca ttttcgtatc ttgaccaatc ggagaaacta
      421 taatcgacgc cgaatttcaa ttttggagaa tgtgtgtata gaacaccaac attaagttct
      481 gacggcaact tgccttttcc tttaactcct tcagcaggtg aaattttgaa tgtatcaatt
      541 gtgcttccgt caggtgattg gatacccgtt cttactcttc tgtaaagaaa tgattgtgtg
      601 gtattaaatg actgtgccga attgccagac acacctataa ggagatttct tcctggatat
      661 tttgcttctt ttttatcatt ggtctggctg aggtcaaagc ggtaggttgc tcctgctttc
      721 caactaaacc cacgtatgct gtactcatcc gtaaatacat cagaataaga ataagcgaga
      781 tcattgaaca aaatctgtct gccatagctt gattttccaa aaagccagcc gatattcaat
      841 ccaacagaca agttcttgta tttgacggca ttaccccaca ccattctata actacctcca
      901 gtgccattat acaatgtctg cacttcacct atgctgtcaa catttcgtgt ttcttgtaga
      961 ttgtagttat ttgtagaata aggagaaaga ttgaatccca ttacccattt gtagtttgaa
     1021 ggttttttct gaagggattc agaaatctga tttttcaacg gaaaagaaat ggacagataa
     1081 ctcatattgc cactccattg tgtctgactt gaagtctgtt gctctatttg ggtatatttg
     1141 gcataaatac caccttcgta agtggcattg atgagcgatg tgagtgttgc tgggttgtca
     1201 aaattggtat tgtagctatc tccatatact gaagatagtc gtcccattcc taccgacgaa
     1261 actttctgcg aattgacgat attgccaaaa ccaaagcgtg aataaggaga attgtctgct
     1321 gactggcaat acagcattac cggaaacaac aagattgcca aaaataaaat tttatttgtc
     1381 attgttctta ttaaaattga gaatggagtt caagcctgtc aaaatgaggt ttggttccac
     1441 tgaatattta ttttcgagtt gcaaagataa aaagtctgca ttaccgccgg ttagtataac
     1501 gttaaattta ccaaagcgct tcttaaaaac ctgaataaag tgactgcatt cgcctactgc
     1561 tcctgtgact acacctgtca tcattgctga aaatgtatca ttgccgtaga aatcagtagt
     1621 tctttttaaa gtaaaagaag gtaagcgcgc agtgaaatgg tgcatggcat caagcctcat
     1681 ttgtaagccc ggacttatgc tgcctcccat ataaccttgc gattcatcca caaaatcgta
     1741 tttgatacaa gtgccggcat caataacaag taccgatttg ccacccaaca tcacccaagc
     1801 tcctaaggcg cctgccagtc tgtctttgcc gagcgtctcc ggtgacaggt aattattgat
     1861 aagtggtaat ggtgtgttat gatcaagctg aataatcttt gtgtctttaa gtaatccaat
     1921 aactgcttca gcttctgatg actcgactga agaaaaaata gtataattta ttttcttgcc
     1981 tgcgatgaag ttcagaaaat ccatttcctc tcctgccaca aaacggatag tgccgagtaa
     2041 cttatcctca ctgaataaac ccgccttaat ataagtattg ccaatatcaa tagctaactg
     2101 catccaacat taatgcttaa aatgccggta accggtaatc atcattgtca ttccattgtt
     2161 gttgcaataa tcggtactgt cctgatcttt gactgaacca ccaggctgta caacagctga
     2221 tatgccctgc ttatgtgcaa tttctacaca atccggaaac gggaaaaagg catcagaacc
     2281 cataactgct ccggtaagtg aaaaaccaaa atgctttgcc ttttgtatgg cctggtttaa
     2341 ggcttctacc ctcgaggtct gaccacagcc catacctaac atcatctggt ttttgacaag
     2401 aacaatggca tttgacttca ggtgtttgac gcatttgttg gcaaactcca tatcaatgag
     2461 ttctgatgct tccggctggc gtgttgtagc tagtgtccag tctttgctta gatgtacatg
     2521 attgtctgca tcttgttcaa gaacaccgtt caatattgat ttaaaactct tttgcggcaa
     2581 tggcgtatct ttcagcttca gcaatattct gttcttcttc tctgtgagta cttcaatggc
     2641 cttgttagaa aaatcaggcg caatgagtac ttcataaaac atagtattga tattttccgc
     2701 agtttcgaca tctactgcaa cattgcttat gatgactcca ccgaaagctg atatcgggtc
     2761 ccctgccaat gcggcattcc aggcatcaat aagcttctct ctggtagcta caccacacgg
     2821 gttcgtatgt ttgagaatag ctatggtcgg ttgcgataat ctgaaatcct gtataaggtt
     2881 gatggcactg tccacatcca cgaggttgtt gtaggaaagc tctttaccgt gcagtttttc
     2941 aattacatca ttcattttac cataaaaata agccttctga tgaggatttt cgccgtatct
     3001 gagctcatct gctatatcta tagcctggtg aaatgaaggc tcatgagcag ttttgttgaa
     3061 atatttgtag atggcagtat cataatgtga agaaacagca aatgccttgc gcgccaatga
     3121 atgtcgttca tcataagtcg tgacaccctc ttgttgctgc ataatcttca ttgcatcaga
     3181 atattgctct acagaaggaa tgacaactac atcacggaaa ttctttgcag ctgctcttat
     3241 cagggaaata ccgcctatat ctattttttc aataattgct tcatcatcat ctgttgatgc
     3301 tactgttttt tcaaatggat aaagatccac aatcaccata tcaatctccg gaatgtcata
     3361 agtctcgagc tgtaccagat gttctctttc tcgtctagca agtatgccac caaatatctg
     3421 tggatgcagt gtcttcacac gaccatcaag aatggaaggg tattgtgtat atttttctac
     3481 cggagtcacc gggatattga gcgactcaat gaatgactga gtaccaccgg tagaaataat
     3541 atcaatacca aggcgatgca tttcctggac aagtggagca agcccttcct ttgaataaac
     3601 tgagattaat gcagatcgga ttttgataga acgtaactga gacatattaa tatttttatc
     3661 gactctcaaa ggtacataga gcaatacaat tttcagtcat ttcttgcctt gtaaagcaat
     3721 cttcatcata gaaattgttg gttttttctg ctatactaaa ataagtattc tcgttatata
     3781 cctaaacttc acctttcatg tatgcgtttt acaaatatca agactagtaa aaaacttgtg
     3841 taaaaatatt cacgttcttc agtatctttg cagacctatg agtcaattta ttgtttctgc
     3901 aagaaaatac cgcccaacca ggttcaatga tgtaatcgga caagaacacg tgtctgttac
     3961 gctcagaaat gctttgcaga cagggcatct tgctcatgct tttttgttta caggaccaag
     4021 aggcgtaggc aaaacaactt gtgccagaat acttgctcgg gtcatcaact gcgagaaccc
     4081 aaccaaagaa tatgatgcat gcaatgaatg cgcttcttgt aaagcattta acgaaaatgc
     4141 ttccttcaat atactcgaac tcgatgctgc ctctaacaat ggtgttgacc aaatgcgggc
     4201 acttacagaa caagtaaggt ttcaaccaca gcaaggcaaa tacaaagtgt tcattattga
     4261 cgaggtgcac atgctcacta cacaagcatt caacgctttc ctcaagactc tggaagagcc
     4321 accgcctcat gccattttta tactcgctac tacagagaaa cacaaaatca tccctactat
     4381 tctatccaga tgtcagatat acgacttcaa gcgaatccag attagggata tggtcggaaa
     4441 attgcaggaa atctgtaagg aagaaggtat tgatgctgaa gaaaaagcgc tccacatcat
     4501 agctcagaaa gcagatggtg ccatgcgtga tgccttatca atttttgaca ggattgtgag
     4561 tttttctggt aaaaaaatca cctacaacga cgtcatcaat aacctgaacg tacttgacta
     4621 tgtctattac ttcagagcca gcgactgtat gatgacagaa gatgccggtg gtttgcttat
     4681 gttgctggat gaaatttcaa acaagggctt cgaagaggaa atatttatgt cgggccttgc
     4741 agatcacttg cgcaatcttt tgatttgcaa agatcagcgt actgtagcat tgcttgaact
     4801 gagcgaagat tttacaaaaa aatatattga aacagcacct ttattcgctg aaatcgattt
     4861 gttgaacgca ctcgctgtca tcaatcacta tgacatacat acccgtactg tcagaaacaa
     4921 aaaactccac actgagcttc atcttctccg ccttgccaat atccgtcatc tgtataaagt
     4981 agatacaagc actactgagg tttcgcagga aaaaaaaact cctgaactaa gccaaccaaa
     5041 aatacaatta tctaataatc aacatgttat aataaaagca agccctgagc ctgcaatagt
     5101 tggtaatcct gctccggctg taccaaagtc cgatactaaa cccgtgttag atacacctac
     5161 ttttttttca cttgaagcac ttgagcaatc aatcatggaa gaagacaatg atcatacaga
     5221 tgcattggct ttaaatgaag aaaacctacg tacttgttgg attgactaca tcaacggaca
     5281 ccaaagccca agtgtaaagg ccatactatt aaatacactt acaagaatag aaaacaatac
     5341 catcgtagct accgtgggta ccacactggc aaaagagatg attctgcagg aaaaaaatat
     5401 cactcaacat cttcgaagta gatttggctt gcccaatcta atgatagaaa tagaaataga
     5461 taccagtctg actcctgagc aggcacctgc aaagccaaaa ccactcacag gaaaagagaa
     5521 atacgagaaa ctcgtagcca tcaacccgga tgtggacgaa ctacgcaaaa aactaaatct
     5581 ccgaatcgac gaattgtaat aaaatacttg ctgaatgttc tatcaatact ttataaaacc
     5641 ggttttcttc tgtttcaacc cggaaacagc tcatgctatg gctatgcgtg cactaaaact
     5701 actatgtgcc attccgggag gcaagctact aaccaacaag ctctttaatt atcagaacaa
     5761 gactttggaa atatcggctt ttggattgaa ttttcctaat ccggtaggtt tggcagcagg
     5821 atttgataaa gatggtaaat acttttctgt acttgaaaac ctgggctttg gctttatcga
     5881 aattggcacc gtaactccca gaccacaaga cggaaaccca aaaccacgct tattccgtct
     5941 gcccggtgac gaggctctta tcaatagaat gggattcaat aacgaaggac tcgaagtcct
     6001 taaaaatagg ctgaagaaca gaacaggcaa agtaatcatc ggtggcaata tcggaaaaaa
     6061 caaggacaca cccaatgaac tggccatcaa cgactatctt gaaggcttcc atcaattatt
     6121 tgatgtggta gattattttg tggtaaatgt gagttcgccc aatacaccaa atctgcgtgc
     6181 acttcaggaa aaagagcctt tgacaaaaat actcagccag ctacaagaat caaatctcaa
     6241 aaaaccaagc cctaaagcta ttttactcaa aatagcacca gatcttacaa acgaacagct
     6301 agatgacatc atcgaaattg taaaaactac ccgaataagc ggagtaatcg ctacaaatac
     6361 aactattacc cgcaataatc tcactaccaa caatacacaa atagaaagta ttggcaatgg
     6421 tggcctaagc ggcaaacctg taagagagat gtcaacgcgg gttatccgtt acattcatga
     6481 acattcaggt ggtactattc ctgtcatcgg tgtaggtggc attcacactg cccaagatgc
     6541 caaagaaaaa ctggatgctg gtgcctgtct ggttcaggtt ttcacaggct tcatctacga
     6601 ggggccttca atcgttaggc gaatcaataa atatctggca aaatcatgtg gcgaaggctc
     6661 tcattctttg taaaaacatt gatggaagaa aaacgtttga ataatattac accacctgtt
     6721 ctttaacaac agttcatgac tcaggatgat ttcagaaaca tttcttcaac attgccctct
     6781 gaccccggca tatacaaata tttcaacgaa gaagacacca ttttatatgt aggcaaagca
     6841 aaaaacctga aaaaaagact agcatcgtat tttgtttcct ccaaaaatct gccatacaaa
     6901 acaagattga tgctacgaca cgcaacacga atcgaataca cgattacaaa gtctgaaagc
     6961 gatgccctct tactcgaaaa cagcctgatt aaaaaatttc agcctcgtta taatgtgatg
     7021 cttaaggatg caaagagcta ttcttacatt tgcatttcga atgagcattt tccaaaagtt
     7081 ttctttacaa gaagaataat caggaatggt tctttatact tcgggcctta tacttcgaag
     7141 ttcagggtca atattctgct cgatttaatc aaaagccttt ttccgctccg caattgcaac
     7201 ctccagctca cacccgataa cattgctaag agaaaattca atgtatgtct cgaataccac
     7261 atcaaaaact gcgaaggccc atgtataggt gccgagtctg aagaacatta caataaaaag
     7321 attgagcaaa taaaaaatat actcaaaggc cacctcaaga tcgtaaaaga ttatctgctt
     7381 gatgaaatga acagatatgc cacaaacctg gatttcgaaa aagcacagca gatgaaagat
     7441 aaattgatag cattcgaaga ttatcaggcg aaatctacag tagtgaatat caacatccgt
     7501 gatgtggatg tattttatat agaaagtaat gacacgactg ccttcgtcaa ttacctgaag
     7561 ataatagacg gaagcattgt caatacacat acccttgaac ttattaaaaa tctggatgat
     7621 gatgacaatg acctgctaag ctatagtatc gatgtgcttc gcgaaaaatt caacagcata
     7681 gcacccgaaa taatagttcc gttcgaaccg attgtttctg atacaagtct gcttgtgaca
     7741 ataccgaagg taggtgacaa aaagaaattg ctggagatgg ctgaaaaaaa tgtaaaatat
     7801 ttcattctgc aacaacaaca ggcagccatc aactccctac ggaaactccc ggccactgaa
     7861 cgcatactga aaaccctaca ggccgacctc aggatgagtg caatgccttt acatatcgaa
     7921 tgttttgaca actcaaatat acaaggaacc aatccggttg catcttgtgt ggtatataaa
     7981 aatgcaaaac cttcgaaagc cgattaccgt cattttaaaa taaaaaccgt tgtaggacca
     8041 gatgattttg catctatgta tgagatagtt acccgccgtt atgcccgaat gctggcagaa
     8101 aacaagactc tgccccaact ggtagtcatc gacgggggta agggtcagct tagttcagca
     8161 gtaagtgcat taaaagatct gggcatactg gagaaattta cagtgattgg cattgccaaa
     8221 aaactggagg aaatattctt ccccgaagac agcattcctc tttatatcaa caaaaaatca
     8281 gaatcgctca agcttattca gcaaataaga aatgaagcgc accgttttgc cattaccttt
     8341 caccgcgacc agcgctcaaa gaattttacc aaaaccgtgc tgaccgacat acaaggtgta
     8401 gggcctaaaa cagccgataa attgctcaag cactttggct catttaagaa aataatggat
     8461 gcctctgatg aagaattgaa gacagtagct ggtgtaaatg tcgcacggct tataaaagaa
     8521 tattatacag ctgaggatgt tgctgaagat aaggatgaag ttacaaatga agattgacgg
     8581 gattgtgtcc ccttcattct tctaataatc ttttaagttc gatgtttggc agtattccgg
     8641 gtatatgttt cttgataccc gccaccattg tctcaaggtg attgactctc tcaggcgatg
     8701 attcaagact cctaacatac gaataaatgg tttctgacaa ttgatttctg tccatgagac
     8761 cttccttgca tgcagaaatg gcataatacc acgaaatcgt tggcagataa gttgctttgt
     8821 tctgataact cttgtctttt tcgagcaaat taaaattcct tactactttg atgatttttt
     8881 catacttgtt ctggttgaga attgcttcaa gatacgctgt aaaaaaaatg tgccatcgat
     8941 atttgaatat ctcctgacgg tatgctttca ggaaggtttc agcataattt tccgcattct
     9001 tgtattgttg gttgttcatc aggcaacgca tatagaaaga aataaacccg attttgttat
     9061 gaaacgaagg tgttagcctc atttcagggt aagcatcacg catcagttga agtgcttctt
     9121 tgtttcggtt ggtgcggagc aatatggcac aaagattatt gacgtaatgt atgtaatcgt
     9181 tgtttttctg gcgaatagac aggtagccat attccacagc cttgtcgtat tccttaaacc
     9241 gggcatgaag tagcactcta ttactgtaat agttgagcaa tattcttttt gaataaaatt
     9301 ttcccttaac aaaccattta tcaatagcat tgtatttttc gacaaggccc tcaaaatcac
     9361 gataattgaa gtgtacaaaa gtaagcctta caagagccat ataccgtatg tagccttcca
     9421 gcgattcatc ataaaacacc tctgtcagcc aagtttccca atggattgat tcatttgtaa
     9481 tactcgaata atgattaata atatcctggg tagcattgtg caatttgtca aacacatccc
     9541 tgtttctgtc gtaaagaacc tgatgtgtag ttatatattg gtctatagtg ttgtgttcct
     9601 gatagcgaag cctgatgagt aagaactgac gatattcggc aagcagttcg aaaaacttgg
     9661 tgaagtagaa catattggtc tcaccggagc gaataagttt aaggatcttt cgctcatcat
     9721 ctgtggtaag gctatcggta agtatgtgtt tcaacgtggt cgttatccag tcaaactgaa
     9781 aatcaacatt tacatctgcg agcctttctt ctatccagtt tttaaggtgc gaatattttc
     9841 gcttgtctat acctacatcg aatagatcgg tttttgaagg atttacagct acctgatgga
     9901 tcgtttcaag cattctaagt ctttcgtcat cctgaaattg ctgtacactg agcaaataac
     9961 tggtttcgtg cggaagtaaa gataccgcaa actcagaaaa tttctttagc ctggttctat
    10021 ctgacaaggt attggattaa ttgccctgca aattaatact ttcggcctta tttacaaaat
    10081 aatgcttact cctgtaccgg attcaactcc tgccaaagca tatcttttaa ttctttaagg
    10141 cctttgtttg ttaccgatga gataaatact gttttgatgc ctggtggcaa atctgactta
    10201 agcatttcct ttagttcatc atcgatcata tcacttttgg ttacagcaag tatccgtggc
    10261 ttatctagca attcttcatt aaacatttcg agttcgtgta gcaatacatt gtactcattg
    10321 ccgatgtggt cagtatcggc cggtaccata aacagtaaaa ctgcatttcg ttcgatgtgt
    10381 ttgagaaacc tgtgcccgag gcctttgccc aggtgagcgt cctctataat gcccggaata
    10441 tctgccatga tgaatgacat gtgcccgtga taaggtacaa tacccaggtt tggcactatt
    10501 gtagtaaaag ggtacgaatc aattttgggt ttagctgctg tgatggctgc cagcaaagtt
    10561 gattttccgg cattcgggaa acctaccaag cctacatctg ctaacacttt tagctcgagt
    10621 attttccatt cttctacacc cggctcgcct ggttgggcat attgtggagc ctgattggta
    10681 gcagacttga agtggacatt accaagacca ccgcgcccac ctttggctat gatgatttcc
    10741 tgtccgtctt catttatttc ggcagttatt tctcccgatt ctacatcacg tacgatggta
    10801 cctattggca cttcaagaac tatatcttca ccatacttac cgaagcgatt attcttggcg
    10861 ccgttgccac catcacctgc tattacatgt tttcggtatt tcagtggaag caatgtccac
    10921 atttgtttat ttcctttgac aataatatga cctcctctgc caccgtcacc tccgtcagga
    10981 ccacctctag gattaatcgg gtcacttgca aaatgcttcg agccggcccc tcctgcacct
    11041 gaacgacagc aaatttttac ataatcgaca aaattttgtt cagccacaga aaagtattat
    11101 caatcggtaa ataaaaatat tattaaatac tgtcgatgct ttcgcatagc ctgttaaaga
    11161 tttcttcaac agttcctatt ccatgcactt tgaatgcctt tcctgaagta gtataatagt
    11221 caaatacagg tttagtttca tcattgtaaa cattaattct gtttttgact atgctttcat
    11281 cattatcatc aggtctgccg gatgtttgac cgcgaagcag tattcttttt acgatttctt
    11341 catcatctac atccagtgct accaatgcat tgatggtctg atttttctca gtcagtagct
    11401 ggtcaagggc ttgtgcctgg gtaatagtac gcggaaaccc atcgaagatg tgtccctgtg
    11461 cttcagggtg tgcttcgagt ttgtttttga gcataccgat ggttacttca tccggtacga
    11521 gctgtccttt gctcatgtat tctttagcga gtaaaccgag tggagtatta ttgcccattt
    11581 catagcgaaa caaatcgcct gttgaaatat ggagtaaatg gtatttttcg accaatttgg
    11641 cagcctgtgt gccttttcct gaccccggag ggccaaataa tattatgttt agcatattca
    11701 aaagatatat ggtaagctaa caaattcctt gcctttcttc aagattgaag gcagaaaaag
    11761 ttggtcggtt agttaaacgc gttgatatta ttcgctgatt acgtctcttg cacctgcaat
    11821 tttcggaaac cagtattcat ctttcattat gttggtcagt ataataccgt tactgttcgt
    11881 actgtggatg accgttatac catcatctgt attggatatt accatggcca catggaatat
    11941 tcgccctccg tcagtgcggg agaaaaaaat cagatcaccc ggttctactt cttttatcct
    12001 ctttgcttta ccgtcaagta tctgtgttcc tgaacttgca gaaaggttga tgtcgaagtt
    12061 tctgaagagg aagcctgtaa atccagaaca atcaaaccca gtagccggct cttttccgcc
    12121 tgatctgtat ctcatccctt caaactgttt tccaagcgac accacttcct gacgaatttg
    12181 ctcttctgtt aatgttccgc ttcctgaagg ccctcctcct gttatttcgg ttgcccgtgc
    12241 agggtagtcc aaagccttta aaggtgcttc tctgaatgcc gaacatgata ccaacacaat
    12301 cgccaacagt agtatttgta acttaatatt ttttactctc atttagtttt atcggttttc
    12361 cattgccgtt caaggccaat aattcctgtt ttcggttgca atattacaca ttacttaata
    12421 aaacaatgta taaattattg taataattaa tgaaaatttt tagtttcaaa attaatataa
    12481 aaataaacgt cgctttagca aattttattt tataaaatta aattaaaatt atctttatct
    12541 gccagggctg ggaaagcatg tctaaatttg gttaaagtag atttgccaag ctctatggtt
    12601 agcacctctt ctctgtcttc acaagatacc atggtattgc ctaaaaaatc aagacataca
    12661 ctatcaccac tatattcgtg gcctttacca tcagtaccta ttctattgac tccggctacg
    12721 aagctctgat tttcaatagc tctggcggcg agcagtgttt tccaggcaaa tgaccttact
    12781 gaaggccagt tggctacgta aataagcaga tcgtaattat ttgtgttcct tgaccatacc
    12841 gggaatctca ggtcataaca gatcaacgga catattctcc agccttttaa ttcatactcc
    12901 agccgctttt ggccgggttt gtagtattta tcttcatggc tgaaagtgaa caaatgtcgt
    12961 ttgtcatagt gatgtattac tccctcgttg gttaccaaca gaagtctgtt gtaatacttt
    13021 tcctcctcat taacaataat cgaaccaatt attgctgttt ctaattttgc ggcttgttct
    13081 ttcagccaga atattactct atcggcctct tttgcatggc cagtgttcat agtaaatccg
    13141 gtattgaaca tttcgggcaa aacaatcaaa tcggttttgc catgaagttt ttcaattttt
    13201 gcttcaatat ttttgagatt ctgatctgta tcttgccaaa ccagatcagt ttgtaggaga
    13261 gttaaacgca atgattgcat gtgttggtac tgtttgtttt tacaaaatta acagtatcct
    13321 tggtacaata cggtataata tgaaaaaacg gatataatta atattatacc cgttttttca
    13381 tatatatatc tgttattatg atttaccttg attacgaaga gctctaggta tatcaatgga
    13441 tgcaatagga atagccggag gattttcaat ggtaatgcca tcctgtgcct tgatgtcacg
    13501 tattctgatg gtactaccta attccattga agaaatgtct gcaaacaatt cgcctacaag
    13561 cttatcaggt gttgttttta cattaacttc tctgagtttt tgtacaaatt tgccaccaac
    13621 tttcacacct ggcgatgcac ctgtaaactt aagtggaatt ttacacttca aggtctgtcc
    13681 ttcgattaac tcaagcaagt ctatatgttc cagtttttca gaaacagggt gaaactgggt
    13741 tgatttaagg atgcacttgt gtgttttacc tccgatttct acttccagta ttttaaaatc
    13801 aggtgagtac actacgtgtc tgaaagaggc agggtctgct tcaaaatgaa ctacatcctt
    13861 tcctccatag ataacacccg ggaccaagcc gttgtttctg gcagatttag cagaagttga
    13921 gcctacttca gctctggtat ttgcttttat tgctactttt tccattggaa aactaaattt
    13981 tgaacggtta aaaaaccacc ttttataaaa aatgaacgca aatatacgac aatttttata
    14041 aaacacacaa taccaaacct taaaatattt ttacttttca ataaaaagtg cagaaataga
    14101 cctatgttcg tgtgtatttc tgattgcccg agcaaaaagt tgtgccgaag acaatacttt
    14161 tatttttggt gaactttgct tgagtggtat agtatccgtc acataaatct cttcaatctt
    14221 tgatttttcg atgtttttgt atgcatctcc ggacaatacc ggatgtgtaa taaaagcacg
    14281 tactgagctt gccccttgct ctattagcat atcggctgcc ttgcaaagtg tacctgctgt
    14341 atcaataatg tcatctacaa gaataacgtc tttacctttt acatctccaa tgacggtcat
    14401 gctggctact tcgttggctc tttttctgta tttatcgcag atgaccattt cacgcgcaaa
    14461 atacttcgca tacctccgtg cacgcttcac gccacctaca tcaggtgaag caaatacaat
    14521 atccggtagt gtgagttgtt ctattgccgg aataaaaatg gcttcactct taaggtggtc
    14581 aacaggtata tcaaaaaagc cctgtatctg gtctgcatgg aggtccattg tcatgattct
    14641 atttgcccct gcagtctcta tcagattagc catcagtctc gcggatattg gcacccttgc
    14701 cttgtctttc cggtcttgcc ttgcatatcc aaaatacgga atgactgccg taatataacc
    14761 tgcagaagcc ctcttagctg cgtcaataaa gagtagcaac tcaagcaagt tatcggctgg
    14821 tgcaaaggtt gattgtataa aaaatacata cgcgccacgt actgattcat ttatcaccgg
    14881 ctgcatctct ccgtcactga acttttggag caaacagtcg ctgagtgggt aaccgtaata
    14941 atcggctact ttttcggaca atggctgtga caacgttccc gaaaataatt tgacttccat
    15001 gtttggtttt tttggtattt tgctttgagt tgcaaagtta aaaatttagc cattcttaat
    15061 atgcaaattt tacggattcg attttaaatt tatggtattc attcacttta cagaaggtgt
    15121 tagctatctc aaagaaacat gacccctttc ttatttaacc cagataacgt attaggaatt
    15181 taaacgaaaa tgataagtac aaaaatccat gaaaaagtga taaaagcata ttgaactttg
    15241 gttattaagc ttttgaaatg gattttaagt aatcattatt tgcagtaagg agcccaatgc
    15301 gttatctggg ttaaaaatct attacggtat aatttgtatt taaccaatgc tttagaataa
    15361 ctttgtgatg gtagttttgt tctttacaca agaaaagcat acaccgatgg atggaaatac
    15421 ttttatgggt caaaaactga tggcggttgt ttttggaatg gtcgctgcag ttatctgtat
    15481 tgttgcattt gacagtatca atcttatggt ttaccctcct cctaagaact tcagttatga
    15541 aaataccgaa atggcgaaag cgtatttgtt caatgcaccg ggttatgcca aactaattat
    15601 catcctcggg cttgcatttg ccggatttat aggaggaatg gtatcggcca aattcgataa
    15661 atcatccgac aaacggacag cttggatagt aggaggcttg ttgttcgtac aatatgttat
    15721 caatttttca tatattcctt atcctgtggt aataattgtt gccggccttg cattggtagt
    15781 acctgctacc taccttggtt actttgtgtt tgtaaaatat ttgaaagtta aggttacaaa
    15841 ataaaatcac tcaactgtat cttgtgcgtg catcttagca agggaaatga taaaagtgga
    15901 acctttgttg agctcactct ctacagttac gtttcctttt atcatatcaa gataacgttt
    15961 gatgataggc aggccaagtc ccgttccttg tatatttgta gcattttttc ctctataaaa
    16021 gttagaaaat aaatgcgaga tatcttcctg tgcaatacca atgccttggt caataatctg
    16081 tatttgaagc ttgttatttg aatcaataat gagtttgatt gggctgtttt cgtatgaaaa
    16141 ttttgttgca ttgctgagta cattgaggag tatgttcttt agtattcgct tgtctgtggt
    16201 gaagctaccg gctcctgtca atacatattt tatggtttgc cccggtttaa gtatcgtttt
    16261 aatttcttca attatttcat aaacaaagct tgagatgtca aaagtctcta ccttgatacc
    16321 aacttttcct tcctcgagtt tgccgataga taaaaaatct tcgagcaatt cgttcaggtg
    16381 ttttacggca tcttttattt tgtttacgtg gcgttctcgg ttaggctggt cttcttcaat
    16441 tctgtatttg gctaccaagg aggccgaaga caaaatcgta ctgagcggtg tgcgaaactc
    16501 atgtgatgcc atgcttacga accgtgattt tatctcattg agttcttttt ccttattgag
    16561 cgaatcactg agttcttttt gagatttttc gagttcgtca agtgcttcgc gtagtatcat
    16621 cgttctttcg aatacctttg attctagctc ggttgtaagt actgtcattt cttctgtcag
    16681 tttttcaagc gagcggttct tttcgatgag ctgctgctcg atatttttgc gctgtgtaat
    16741 atcaactata aaagcaaaaa ccaggtaact attgccgtgt ctgaaatagc tgaggcttat
    16801 ttcgaccgga aattcctctc cgttactttt aaggccggac agatcacggc ctactcccat
    16861 ccgtctgttg gctggttggt tgaaaaaatc ggtaacatac agattatgct ttggccggta
    16921 gcgttccgga atgagtacaa acagattctg cccgagtaat gactcagatg agtatccaaa
    16981 aagattctca acagccttat tgacataaat tatcttgctc tcatccgaac acaccagaat
    17041 accttctgta acactgtcta ttaatgaccc caataactct atgctgaacg gttccatctt
    17101 acttaactgc aacaattatg tattaataat ttattcatcg aacaattttc cctggccttt
    17161 ctgtggcgga atgtcaaaat ctatctggtc accagggtgc aatttttcct gcataggtgt
    17221 cggttctgtt atctttatat ctgcctcgag aagtgggtag tcacatactt tgttgccgat
    17281 tgatttccaa cctttgaccg ttacgaagtc atctagttct atttcctgtt catttatttt
    17341 ttttccactg tcccatacac aggttagtct tacagctggc gtaagggtta cgtaatataa
    17401 ctttgaatgc ttatggtcgg taataaaacg gtatcgctgg ccggtagaag tagcttctat
    17461 tttaaaccgc ttaatcatgg accatccttt ctcggcttcg taataaagca cattcaagat
    17521 tctgtccggg tcaaatttat tcaggtcgat cagtttttct acttcatacc tggtggttgt
    17581 gtcgggttct gaaatcaaat agctaccgtc ccggtatatt gacaatatca tatctccggt
    17641 atcaaacgca ccaatcttcc taccacgttc gtcggtgttt agtttgccgg ttgtctcgtc
    17701 aaaccaaaac actttggctc caagtgttga cttaccgagt tctttttgta ttacttttct
    17761 taccggatat ttggtaattg tattcccttg cgaatttctt cccttgatag ccaactcacc
    17821 aaagtcaaat tcaaactgct tgatacgtgc actgctggta gctgataagc ttattgttac
    17881 agtttctgat tcgccgtttg ggttagccga aaaatagatt actttggagt taggtgtacc
    17941 ttgagtgagg tcgtactctt tatcgcgtgt tatgcttgtg acgttaaagc gcttggcgaa
    18001 ggttttgcct gatctgccat caagatagat taagttgtat gtagtgcgct catcattttt
    18061 cttccagaca ttggtatata tcagatcttt gcctacaaaa accttgtcgg ctatccttac
    18121 tacgctgaat tttccatctt ttctgaatgc gataacatca tcaatatccg aacaatcggt
    18181 tataaattca tcttttttca agccaaatcc tacaaatcct tccttataat tggcataaag
    18241 ctttgcatta ttggctacca cctcctgtgc gatgatagat tcaaaatttt tgatttccgt
    18301 ctttcgttct ctgcctttgc cgtatttttc ctttaatctt tggtaataat tgatggtaaa
    18361 atcgttgagg tgggcaatat tgtatttggt ctcttcaagg tctgcctcga gttgtctgat
    18421 gtgttcgtct gctttgaatg aattgaattt ggtgatacgc ttgatcttta tttcggtcag
    18481 tctggtatag tcttctgtgg tcagttctct aagcaatttt agtctcctgt ctgttttccc
    18541 gttgtagtca gacggagtgg caacgtattt tttcagtccg gcttctatgg tggtcattac
    18601 ttcttcccag gtttcacact cttcgatgtc atggtagatt ctattctcta tgaatatttt
    18661 ctcaaggctt gcaaacagcc agctttcttc aagttcattc ttttttatat caagctccca
    18721 gccaagtagt tttttgctga attctgtagc attcttcagc atatcactta cagtcacaaa
    18781 atggggcttg tcatcaagga taacgcaagc attcggcgaa attgatactt cacatgcagt
    18841 aaaggcatag agtgcatcca tggtaacatc tggtgatacg cccggcagta attctatcaa
    18901 aatctctact tcagctgctg tgttgtccga tattttcttt atttttatct tccctttgtc
    18961 cgctgccttc actatacttt ctataagagc agtagtagtg acaccataag gcagctcacg
    19021 tattaccagg tgttttttat cacttgcatc aatcttagca cgtactctta tctttccccc
    19081 acgttggcca tcattgtagt ctgcaacatc tacactgcca ccggtcaaaa aatcggggta
    19141 caactgaaaa ggctcatttt gaaggtaagc tatgctagca tctatcagct caatgaagtt
    19201 atgtggcatt attttggtcg ataaacctac cgcaatacct tctacgccct gcgcaagcaa
    19261 caacggaaat ttcattggca gtacaacagg ttcattcttg cgaccatcat agcttagctg
    19321 ccattctgtt gtctgcttat taaatgccac ttcgagagca aattttgtca gtcttgcttc
    19381 aatatacctg ggtgctgctg ctccgtcacc tgtgcgtatg tcaccccagt taccctggca
    19441 atctatgagc agatctttct gaccgaggtt aaccatagca tcacctatgg ctgcatcacc
    19501 gtgtgggtga tattgcatcg tctggcctat gatattggct accttgttat aacgtccgtc
    19561 gtccatttca cgcaaagcat gtaaaatacg tctctgtaca ggcttcaatc cgtcttcaag
    19621 tgccggaacc gctcgctcca gtataacata cgaggcatag tcaagaaaat aactcttgta
    19681 catgcctttt actgatatga tttgttcttt ttgcgtttcc agatcctttg aagggtcata
    19741 ctgctggctc atcttacaat attgttgaat gtgcaaagtt acaaagaaat tggctgtatc
    19801 tgctattttg aataactctt gctcagttga ttacctgatt aaggtatttt atgaaaattg
    19861 aaatacaacg tgcgattgct atttttttac actttaatgt tagaaaatat tcggccaccc
    19921 acaacaaccg tggacacttg actgaaaaac agctttaatt ttactttttg gtttatcttt
    19981 atcggtcaaa ggcagaggta tttatgctaa aatttatttc caaactgttt aatattctac
    20041 tttttagaat tttgttggct atttcccgac tgccttttag tgtactctat accttgtctt
    20101 catttttatt cttcttgtta tactatattt taaaatacag aagaaacatt gtaagacaga
    20161 atctgctagg gagtctgccc tcaactataa atccggttgt cattgaaaaa aatttttaca
    20221 caaatctatg cgaaatgttt ttagagtcgc ttaaatgtat gaccatctcc gaacacaata
    20281 tcttgcaaac cattcattgc gacaatccgg agctcgctaa caaatatttc cactccggca
    20341 aaagcatcat cctcatgtct ggtcatttct ttaactggga aatcctcatc tacagcatga
    20401 atcttttgtt ccaccatcag gcaataggtg ttggaaagaa actcacaaac caagagttca
    20461 accggctaac caatgagcga agaagcagat ttggcctcaa catcatacat gccgacaaca
    20521 tccgtgagga gtttgccaca ctggcaaatg ctaatactgc tacgctgttt ttatcagacc
    20581 aatatcctgg tggtgctaca aaaggatatt ttaaaaaatt cctcaacaaa ccatcgtatt
    20641 tcctttatgg tgctgaaaaa tatgcccgac aatacgatct tccggtatta tatgctagat
    20701 ttgagaaact caaacaaggt agctacaaaa tccatctcac agaaataacc gataagccga
    20761 atgacactga ttatggattc attatcgaag agtatgtccg tctccttgaa caaaacattc
    20821 tggaacaacc tgaaatctgg ctttggtcgc acaaacgttg gaaaaatatc gaaaactatt
    20881 accacaatta aacgacaaca aaccattggt cagattttat ctacatttta tctgaaatac
    20941 atttgacggt ttgttttttt gtgaaaagaa attttaaaat ttgcgcctta aacctaagct
    21001 ctttatgaaa tcaaagctgt ttttaacact gataagcctt gctatctgtt tacatccatt
    21061 tgcccaaaca tcttacttcc aacaacaagt ggattataag attgacgtaa cacttgatga
    21121 cgaacaccat ctgctacaag gttggataga aatggaatat accaacaaca gtactcaaac
    21181 acttgataaa atatatatgc atctgtggcc aaatgcttac aaaaatgaca aatcggcatt
    21241 cgcgaagcaa cagttgaaaa acaacaaacg ttcttttcat ttctcagaaa aaaaagaccg
    21301 cggatatttg tcaggtctta attttactgt aaatggtacg acaacagtca tacaggaata
    21361 tgaagggaat ccggatattg tgctgctcac attgcctcaa ccgcttgctc cccaccaaaa
    21421 aataagcatc aaaacgccat ttaccttaaa gattccgaaa gttttttcaa gactcggaca
    21481 taccgggcag aattatttca tcacacagtg gtatcccaaa cctgcagtat ttgacacaaa
    21541 gggctggcac ccaattccgt atctcgacca aggcgaattt tactccgaat tcggcaactt
    21601 cgatgtaaag attactttac ctgctaacta tgttgtagct tcgacaggta ctaccacaga
    21661 tgataccgaa ttttttaaca acagagtaag cctcactaca aagctgcttg ctaatgcaga
    21721 tgctgccggc aattggaagg cttcttcatc tgagggaagc aaaacgctcc attaccatgc
    21781 tgagaatgta catgatttcg cttggtttgc aagcaaggag tttatgatag taaaagatac
    21841 cgcacgactg gaagaaggca accaactgcc tgtttatgga tatttccttt tgaaaaacaa
    21901 aaagaactgg gccaggtcag ctttctatgt aaagagagct gttgaatacc gctcacgcag
    21961 gattggcgat tatccttggc cgcatgcttc ggctgtagat ggcgacctga tagcaggtgg
    22021 tggtatggag tacccgatgg tcaccgtcat cagcgcaggt ggcagtgctc agagtctcga
    22081 taacgttata gaacatgaag taggtcacaa ttggttctat ggtattttgg catcaaacga
    22141 acgcgacgac gcatggatgg atgaaggtat caattcttac tacgaagccg actacatgac
    22201 cgagtattac aagcccaaaa acgaagctca tgtgcaaaaa aaacgccctc gcggattttc
    22261 gttagatgaa agccttattt cttccatttt tatgaaacga aaacttgacc agcctcttag
    22321 cttaacttcc gctgaattta tcaatgtcaa ttacggcctg gatgtgtaca agaagaccgc
    22381 ccggtatttt aagatactgg aactctacct tggtacaaaa tcatttgatg ctgcaatgaa
    22441 agaatattac aggcaatggt catttaaaca cccatatccg gacgatctga aacaggtact
    22501 cgaaaaatca acaggaaaga acctgagctg gctgtttgac gacctgattc agaaaaatat
    22561 gccggttgat tataaaatag taaaagcctc caacaacagt gtgaccataa aaaacattgc
    22621 cggcaatccg acacctttga acctcacagt tactaacaaa aacgggtcaa gcgctactta
    22681 tttttacgag ggctttaaag acgttaagtc aatcgatcaa gatatggcgg aagtgaagtc
    22741 tctgaccata gatgccgata actacctgat ggatgccagc acttccaaca attattacca
    22801 tacgtcgggt atctgtaagg cttcaagacc atggacgcca aagatttttg gtctgataga
    22861 acaaaaagat aaaaatgtca cgaacatatt gcctgctatc ggttggaacc tctatgacaa
    22921 attcatggca ggtattttga tctctaaccc gattttgccg gggcgcaatt tcagatattt
    22981 tgttgcaccg atgattgcga cgagtgatgg ttctgtagtt gggcagggca tgctggaaca
    23041 aaatttttat tcatataaaa acccagaaag actggcaata ggtatttcag gcaggagata
    23101 tgctttcaat ttcattgaca attctttgtt aaaagagcga ctccattaca tcaaagtgac
    23161 accatacatt acagccgaat ttaaccgctg gcatacctgg gcacacaaac tgacctacaa
    23221 aacaaacatc cttgtcaagg accaggtaaa tataaaacaa gtcgatggtg cagtaaaagc
    23281 agaagtaaaa gccgacaatc agtatatcca tacccttgat tatacactta ctacacacaa
    23341 tgggctttca cctatggaag ccaacataag acttgaacag caaaactaca acagtacaaa
    23401 aggaaaagag cattacctga agctctcgct cgaatacaat acaaaactgt attacaatac
    23461 aaaacgctct gtcgatatcc gaatatttgc cggaggctac cttgacaata ctgcaaaaaa
    23521 ttcaggtgct accaactcag attattttgc ccgtggctcc atgtctctag cttcaacagc
    23581 cgccgacgat tatctttttg agtgtccgta tctgggcaga tcagctgacc agggattctt
    23641 ctcgcgacag atagatataa gcgatggtgg gttcaaattc cctttagcct ataaatatgg
    23701 tcaatttgga tacaccaatt cttttctggc tgctttaaac ttaaaagcag gccttcctgt
    23761 agatatgccg cttaaactgc ccataaaacc ttactttgac cttggttact acaacgacct
    23821 tagaccaata aacagtgaca agaattggaa agacaatgtt gtttgggctg gtggatttat
    23881 gctcgacttt ggcagctatg ttggcatata ttttccggtt gtccagtcaa aatccctttc
    23941 agaccagtac aatcagtcag taggtgccaa ttatctaagc agaatcagct tcagtatcaa
    24001 gttaccccag ctacggatat cagagttggt aaaatcattt agtttttagt catcaacttt
    24061 aggatagttg gatgggagtg tgaaagaaat taatcacatt cccattcgta tttttgtagt
    24121 tgattattgg ctgccagccc atactgaaaa tgccaccact ctgtacggat acctgtaaaa
    24181 cctgcctgtc gcataattga gcagagcagg tatctgttag tacgggcatt atcgtttatt
    24241 tcttttgaaa ggtaatatga ttttttcgaa aattcatcga attccgaacc catatccaga
    24301 taattaccat cctggtctgc caatgtaaca tctacagcta ttgctcttgt atgtagtgaa
    24361 cctttccacg gcctcatcac aaagtgtggg tctggtttag cattccataa tttttcctga
    24421 acagaatgag gtctgtaaca atcaagtaat acgagtttta gttttttctt gcgaaatagt
    24481 tcattagcct ttctgagtct gtctgccact tcagttttca ggatacaacg tgggcagtcg
    24541 tagagccttg ttttgacaaa attattgtca cttgcatacc gtaagtcaag tataaatgaa
    24601 gtgtctgttg gagctatttc tgaaaaacct gccggaacgg gtaactgttt gtgtgtaggt
    24661 aattctgcta ctgctctgct aatcgtatct gcagaatgaa cagacgaagc agtttgatca
    24721 atcgctgtat gatgatactg cctctgccga cacgatatcg tcagcagagg cagtaagtat
    24781 atgaacaaat agacaatccg tttatttgcc gccaagtatt tcttccagtt ttttaccaag
    24841 tacttcacct ctaagctgtc ttgccactat cctgccatct cggtccacga gtacggtatg
    24901 cggtattgat gtgatgccgt atagagcagc agctgagctg ccccaaccct tgaggtcact
    24961 tacatgtggc caggtcagtt tgtcgtcagc tatagctttt atccacggct ctttgcttcg
    25021 gtcaagactt acacctagta tttcaaagcc tttgtccttg tacctgttgt acattgctac
    25081 tacattcgga ttttctcttc ggcacgggcc acaccatgat gcccagaagt ctattaacac
    25141 tacttttcct ttgaaatcct taaggctaac tttttttccg tccggcgtct caagcgtaaa
    25201 atcaggtgcc ggcaacccaa tcatcaattg tttgatggca tccagacgag gctttagaag
    25261 accaatatcc atagaattgg ttttagcata tttggtcgaa aactcatctg caaattctac
    25321 aaaaagtacg tctgtatttt gttcaatgcc agatactaca ccaccaagtg ccagcctata
    25381 agtgttagat gtctgaggta ttttcttaag ttgggttctt attgaattaa taatataagt
    25441 atttcctcta cctactcttt gaagcgtctg tgtatatgcc ttgaatattt cggtcaccca
    25501 tggattttgt tcaagattct tatcatctag tttgctttgt ttgaaaaatt catttgcgaa
    25561 ataatcaatt tcatcagtgt atttgccctt gttgttggca aagctgagat aagtgttgat
    25621 agctgccgta tttcccagaa atgcatttat cttcataagg gaatccttaa gtgaaatttt
    25681 ccttttgtcc aagtctgcaa gcgacttttt aaaatcagct tcattttttt ctgtacgttg
    25741 acttgatgta taagtcgaaa agatttgatt ttcctgaaaa cggatttctg ataatttcat
    25801 caatagtcca tcataagctt tgttaatcgg cgaacctgga atatccgatt tttttacggc
    25861 ttcgcagaca acattaatgt ttacattatc ttcagtgcca agtatcacag ggcgaaggtc
    25921 agagattgag aagcctacat agtacattcg gggagtggtt gatttgttta gctcgaactt
    25981 ggcaacccga ttttcgcctt ttgctgcttt taccatatca aaactcattc cgttgaattc
    26041 ataaagatta atggagtctg caccacaatt tttcaaatcg cagctcaatg taatattttt
    26101 gctttggccg taagccaata atgacccaat ggttaataaa agtgtaaaaa aatgtctcat
    26161 ttgttgtgtg gtcttaatta tttattagtt ctaaaaattg ttgttcagtc aaaatttcta
    26221 ccgtaccaag ttttttagcc ttctcaagtt ttgatcctgc attttcacct gttaccaaaa
    26281 tgttgagatt cgaactcacc gcacctaaat tcttggcacc tgcattttcg gctaattttt
    26341 ctgcttcttt acgtcccatg gtggataatg ttcctgtaaa gaggatagtt tttccgctaa
    26401 gtaacgtgtc ttctctgcca attgttggtt tatcttcatt tgttgacaaa aaattaacac
    26461 ctgcagactg aattctttcc agtagttcta cattttcgga gtttgtaaaa aatgttcgtg
    26521 tattctttgc cactttcggc ccaatatctt taattgccaa ataacgctct ataggccact
    26581 ccttcagatc cattacatta tcaatctgac cggccagtag tttggatgct tttttaccca
    26641 gatgatggat actcagacta tgcaaaaatc ggtgaatcgg attttttttt gctgtttcaa
    26701 tggcttttct taaattttcg gcagattttt taccaaaccc gtcgagtttc gaaatgtctt
    26761 cataatcaag cgtgtaaata tccgccatat ccttaatcca gcctagatcg taaaattttc
    26821 taattaaaga ttcacccaag ccttcgatgt ccatagcgtc ttttgaagcg tgaaatataa
    26881 gtcgctgaat gattttctca tgacatttct catttataca cctccaggca gcctcccctt
    26941 ccacacgtac caatctggta ttgcacgaag gacaattctc cggaaaaatg attggaacct
    27001 catcaccttt gcgtacatct gcaagcgatt ttacaatgta tggtatcaca tcacctgctc
    27061 tctccagaag gaccatgtcg cccaacctta tgtcgcgtga ctggataaac tcctcattgt
    27121 gcaatgaaat ggagctgacc gtgacacccg ccagagatac cggagttact ttggctaccg
    27181 gagtgacact acccattttg cccacctgaa actccacatg ctcaagcctg gatgttgcct
    27241 gttttgcctg aaatttgaaa gctatcgccc acctcggatg gtggctggta tatccgcaca
    27301 gttcctgtat tttcctgtcg ttgaccttta tgaccatacc gtctatttca taatcaaatg
    27361 aatctctttg cttttgccaa agctgacaga attcttctac ttcgtgaatg ccatggcaga
    27421 cctttctctc gtttacagga attttgaagc cgagttggcc gagtagttcg atgccctcat
    27481 catgtgtcgg aattgtttgc agaacgtcat tatggttgct gtcaaccgcg aagccaagct
    27541 gatatacaaa cacttcaatt tttctatcgg cagtttctcg cggatctttg gtacgcagac
    27601 cgcctgtagc tgcatttctt gggtttgcaa atcggctgag tccttcctct tctcgttctg
    27661 catttatttt ttcaaactga tctttccgta taagtgcttc accacgtagc tctgccttta
    27721 ctatattatg gtcagaaaat gctaccttgc gcggtaccga cttcaatgcc agaatattat
    27781 tacttatatc ttctccattt tgtccatcgc cacgtgtagc agctcttatt aaggagtcat
    27841 tttcaaaaac aattgcaatg cttccaccat catattttgg ttctacacag tattctatct
    27901 tttttgccgg atctatgcca gccagctttc ttatctggtt gtcaaactct atcagatcat
    27961 tcaggtcata agaattagcc aatgaaagca taggcgtaag atgactgaca gtctcctggc
    28021 ctgttgaaat atcgggagaa atacgctgag taggcgaatc agcaataata agatcggggt
    28081 gttcttgttc tgtttcttgc agttttttat acaagacatc atactcatag tcactgatga
    28141 gtgggtcatt catgatataa tatcgccatt cgtgaaatct gatgagttcg cggagctgtt
    28201 cagcaaattg tacaggtact tggtgtatgc catattctgc atcagacaat atcttttttg
    28261 tcagctctgt tagtgtttgt tgttgttctg gagaatacat aatcctatta tattagtaca
    28321 aaattaatca cttatcgtct tgtatgataa aaaaaccatc ttctcctttt ctttggtaca
    28381 atggcatcaa aatatatcca ttataatctg aataaatatt gccattttta tctcgtgcaa
    28441 gtagctctcc tttctttatt ttctgaaaat tttcaaaacc cggcttcatc acaaactcat
    28501 cagctctgat aatctgatgt cggtgaataa gtctggtcaa tgctggtaaa tgttcggaat
    28561 atgctttgag tatttcgtca tgctggcttt ctacatcatc atggtcaaca cagccaatgg
    28621 tgcgaagaca gttgacaaca gcagctatca ttctgtttac ggaaagaggg ttggtatgtt
    28681 ggccggcttc aaaaacaagc ggtataaccg gctggtcgta cttataccca ttattgctga
    28741 aataatgtag tgtggtccct ttaagcccac tcatgaaacc ttttataacc ggagcgtgaa
    28801 gggccttggc tatcctgata ctttcttctt ggtcactagg tatggcgaac aatcctcctc
    28861 gtgcagatgt ggtgtgcaaa tcgagaataa ccacctttgt cggcaatgtt tctttgataa
    28921 attcatcaat ggttgtaagt aattctctaa gttcaagctc ctcagcttcc aaatctgaaa
    28981 aatttgcttc taatatcctt gtaacagatt catctaaaaa catccggttc aggtcagatt
    29041 ctataaaccg cgtttgactt tcactagctt cgacattgcc taccagccca attacagtac
    29101 cagtaaaaac aaaatgtggt ttggacgcag tttcctgtac taacatctct tccaggcgtt
    29161 gcaatgccct gatacctgct atttcattgc cgtgtatgcc ggccagacaa atcagcaaag
    29221 gcccgggcaa tccttgggta tatttaaata tcagtctttc catgccagat cttttgattt
    29281 ccagatgtat cttgacgcaa acgatctgta tggtttccat cgttctgcaa cctcattcat
    29341 atccttgatg agctgtttgc cagttgattc gagaccgtag agttttatca cctgttgttt
    29401 aatgccaaga tcatcataag gaaaaacatt cggccgtagc agagaaaaaa taagtaccat
    29461 ttctactgtc catttgccaa caccttttat ttgtgtcaac aaagacaata tttcgtcatc
    29521 agacatactc tgccagcttg ttgaggccag agaattttca atccagaaac cggcaatatt
    29581 gtccatgtat ccggcttttt ggtacgaaag accaattgca cgtttttctt ccgtactcaa
    29641 tcgcagaata tcttcaggag agggatcaac tgtgcccagc aaatcatgca atctttgtgt
    29701 aataatggcc gctactttta cagataattg ttgcgaaatg atactttcgg taagttcttt
    29761 gaacacattt tgctgaacta tcaaaggtgg tagaacaatc ttttccacta ttggttgtaa
    29821 cacagaatct tgcttcaaat ggcctttaat tacttcgata tccataaaca gatgttcaaa
    29881 cttgcggatg ttaacaaaat cacttaattt cgtctgctaa tcttttcaaa gatataattt
    29941 acatttctat tatgaaaaaa atattgttgt ttctcttctt ttcacttgca ttaagttcaa
    30001 taatttttgg tcaatccggt tggaaaacaa agcttgctcc aaaggttttg acagaatacg
    30061 ttgaagataa cgaacttgac cttattgtag tatttaaaga caaggcagat gtttctgttg
    30121 cgaaatacct tagtaccaaa tccgctaaag gaaggtatgt ttatgaagca ctgaaggcta
    30181 ctactcaatc atctcaaaaa aatgttatgg cttgggcagc atatcatcat tacaaaacaa
    30241 agtcatttta cataacaaat gccctccaga taaagatacc tgctgcatca cttgaacaac
    30301 tggcttcatt cgttgaggta aaatacatca gtaataatgg tctgcttaga tatcacgaac
    30361 cggtagagaa gtatccggca ttgctacgag gcaatgaagg aaaagaatgg ggcattgaca
    30421 ccatcaatgc caatgatgta tgggcacttg gctacaccgg gcaaggtgtt gtggtaggag
    30481 gagaagacac cggctacgac tggaaacatc cggccataca accaaaatac cgtggctggg
    30541 acggaaccac agctgaccat aactataact ggcatgacgc tatacacgaa ctcaacccac
    30601 tgaatggtga ctctacaagc aacccattaa acaacccttg cggacttgat tctcctgaac
    30661 cctgtgatga tggcagccac ggtacccaca ccatgggcac aatggttggt tcaacaccag
    30721 gcgataccat tggagtggca cctgatgcca ggtggatagg tgtaagaaat atggaacgtg
    30781 gctggggcaa accttctaca tacatagaag gcttcgaatg gtttctagca cctactgacc
    30841 ttaataatca aaaccctgac cccgataaaa gtccggacgt tataaacaat tcgtggggtt
    30901 gtcctccgaa tgaaggctgt gatctttcca atttcggtgt tatgcaggat gtagtagcca
    30961 atctaaaagc agccggtata gtagtagttg tttcggccgg taatgctggc agtggctgtg
    31021 gctccattga tgaccctgca gctatttttg aagagagttt ctctatcggt gccaccagat
    31081 caaacgatac tattgctaac tttagcagtc gtggcggtgt tagtatcgat ggaagtttca
    31141 gaactaaacc aaacgtatct gctcctggcg taggcgtcag gtcttgtact ccgggcggta
    31201 attatcaatt cttcagcgga acaagtatgg caggtcctca tgtagcgggt gctgtggcat
    31261 taatactcag tgctgcacca gaattgaaag gtcaggttga tgttattgaa gatatccttg
    31321 aacaaacagc catacccaaa tatactgacc aagagtgtga cagcattccg ggttctattg
    31381 taccaaacaa cacttatggg tacggccgca tagatgtact tcaagccgta aaacttgcct
    31441 tgaaaacagt agcagctaat gatattgaaa aaccgaaaac tacagtaaca tctttcccta
    31501 atccggtaag caataccttg tattttgatg ttaaggagtt taacggagat atacttatcg
    31561 aaatatttga tgtgcctggc aaactcattt attcatcaaa acaacaggta gcaggagaca
    31621 agcttgtaaa atgcgatttt caacatgaaa gtacaggttt gtatttctat aaaataaccg
    31681 ggcaacaact ctgtgttgct ggtaaatttg tgaaaaacta aaataaagca acaaaatatt
    31741 aattgacagc aagaatgcaa cttgccgata tttgttaata ataattgttt gaatatgaga
    31801 ctttacaacc gcataaacgg catggaactc aagcgccgga tgcaggatag tactgagaca
    31861 cgtactacca tttcgtttta caaatattac cacattaaaa atcctcgcct gtttcgcgac
    31921 cattttttca ttctactcga caaactggat gtgcttggca ggatttatgt ggccgctgaa
    31981 ggtgtcaatg gacaaatgtc tgtaccaaca gccaacattg aaaaactccg ggaagcattc
    32041 gataccattg atttcttcaa aggaattcgc ttcaattatg ctattgatga taacggcaaa
    32101 tcttttttta agctagccat taagctaaga gataaaatat tggctgatgg cctgaatgac
    32161 aaaagctttg atgttacaca gaaagggaca catgtagatg cagcagaatt taacgctctc
    32221 gcagatgacc ccaatactgt tatcattgat atgcgtaacc actacgaaac agaagtaggc
    32281 cacttcaagg gtgccatcac accggatgtc gaaacattca gagaatcact accgataata
    32341 gaagaaatgc ttcaaaacca caaggaacaa aatctactga tgtattgtac cggcggtatc
    32401 agatgtgaaa aagcaagcgc ctacttccgg tacaagggtt tcaaaaacgt ttttcagctc
    32461 aatggtggta tcattgaata tgcccggcag gtacaagaac aaaaactcga aaataaattc
    32521 atcggcaaaa attttgtctt cgacgaacgc cttggcgaaa gaatcagccc tgaaatcata
    32581 gcttcttgcc accaatgtgg aaaaccgtgc gacgcacaca ccaactgtgc caatgatgcc
    32641 tgccacttat tattcataca atgcgacgaa tgtgctgcaa aatatagcaa ttgttgctct
    32701 accaaatgct gcgagttcaa ccaattggga gatgataaaa agatactccg aaaaactgaa
    32761 gtatttaacg gtactcgatt tggtaaaggc agatataaag ccttcaacaa agatcattca
    32821 ctcgatatga gctaagacgg tttttgtcca acttactaag ttgcatatta acatttattt
    32881 atgcattttt tgattcaaaa aaataataat ggcaaaatta atcaccgaca agccacttgc
    32941 tcaacacatt gaaagtctta tcttttcatc tgaacagcca ttggcgtttg atgaaataca
    33001 ccaatgtctc gaacaggcgc ttgagctcac actatcaaag aacgaaataa aaaaaaccat
    33061 cgatgccctt gtagaaagat atcaggcacc agaattcggc atcgaaatcg tcgaaataaa
    33121 tgatgggtac cagttcttaa ccaaaggagc ttatcaccac accatcgcta ctttactcaa
    33181 acaatataac aagaaaagac tttcggtagc agctatggaa acgcttgcca ttatcgccta
    33241 caagcaacct gtatcaaaaa ccgaactcga gaagatacgc ggagtcaatt gtgactattc
    33301 tgtacacaaa ttgttagaga aagaattgat aaccatttct ggcagaagcg atgcacccgg
    33361 caaaccaata ctatacgcca ccagcgaaaa atttatggat tattttgggt taaaatctat
    33421 tgccgattta ccaaaactca aggattttca gtcaccggaa aatgaaattg gctttcagca
    33481 ggatatagaa ataccggcca aataatggta aaaatgattg ccaaattttc ttttgaacca
    33541 gattattaaa ccaaaagaga ctttgctccg ttatcatatt tatggaaaat actcaaaaca
    33601 aactggtaaa cagagtagct gagagtggac tcataaccct gaacctggaa gactacttcc
    33661 ctgatactac atttgcgcat ttcgatatca aggactatct gttcatggaa ctaatactga
    33721 aagaaaagga tttcagagaa gcgatgaaac agtacgactg ggcttcactc gaaggaaaga
    33781 ccttattgct ctattgtagc aatgatgcca ttatacctac atgggcatat atgctggtga
    33841 ccaccaatgc acaggcttat gtcaatgata tattccaggg cagcgaacag gagtattaca
    33901 gatataagat gaaaaaaatc atagaagaca ttgattttag caaatttgac aaaaaaagaa
    33961 tcgtagtaaa gggctgcgga aaaaaacctg tgccggtagc tgcttatata gaaataacaa
    34021 gaggccttaa accttatgcc cagagcatca tgtacggcga accttgctct acagtacaga
    34081 tattcaagcg accccgtgag attaacaaac ccgaataaca acacaggata atgcaggata
    34141 agaaaattta tgtttacggc agtttggttt ttctggcatt tttgctcgga atcaacatgc
    34201 aaaatactac ccgtggcaca ttgccaaagg ggcagtattc atttaccgac gtcaagacca
    34261 ttgacctcaa caaagattat aactttgccg gtgaatcagt acccaaagac aactttgatg
    34321 cactcgaaag actagaacgt gagcttctgg taaatgctta ctggcactca aatacattga
    34381 tgatggtcaa aaaagctaaa cgatattttc cggctatcga aaaaatactt aaagaacaag
    34441 gcgtgcctga tgacatcaaa tatctggctg ctgccgaaag ctcattcaac aatgctactt
    34501 ctccttctgg tgccaaaggt atctggcagt ttatggagtc aaccggtaaa gaatatggca
    34561 tggaaataaa caatgaagtt gatgaacgca atcatttcga aaaatcaacc attgcagcct
    34621 gcaaaatgct cacaagatta aaagataaat tcggcagctg gaccctggcc gcagcagcat
    34681 acaatatggg tggcaatggt ctggccagag aaatggacaa ccaacgagcc tctacatact
    34741 acgacctcaa cctcagcaac gaaaccctgc ggtatgtact acggcttatt gccattaaag
    34801 aaatatttga agaccccgca cgttatggtt tcgctactga tgacggtccg ctttataaac
    34861 cactcgataa ctattacgaa ctgagtgtgg acacaacaat aagcaacttg ggcgactttg
    34921 ccaacaaata caatacatct tacagaatgc tgaaagtgta taatccttgg ttggtatctt
    34981 atagcctaac caacaaggac aaaaaacaat atctcattag gataccgaag taatatcata
    35041 atctgctgtt ggcttatggt tgctgacaag tagaaatcat tactttttct tattgtacga
    35101 tgctttaatg ctgtctatct tatcctgatc gggggcattg tgacttggtg cctcagttat
    35161 tttatctttt gaaatatttt tttgccggct acacgccaca atgcctgtaa caatgagtat
    35221 tgcaactacc aataggcttt tattcaacaa caacaacttt gacttcattt ttataattat
    35281 tatctccaag tatagtaaga atataagtac cggctgtaag attattgaca gtaaatgaca
    35341 atttatgctt acctgccttc aaaaaatcct taaatagctc ccttatcatc ttcccttcca
    35401 aatcgaccaa ctcaatagta atccactgag catctgccaa ttgaaactca tggttaaaaa
    35461 tatctttaat cggtacagga taagttttcg taggggcttc tgtcagggtt ggctcatttg
    35521 atgctgtttt taaggcaaaa atctttgcaa ttttactcct gaacgtattt ttagtactcg
    35581 gtgaactaac attgcctcca tacatgccgg tcatccatag tacaggatat tgtgcattat
    35641 tgacaaactg acggcacact gttatgtagt cgccccagcg ttcggtagta gatgttacca
    35701 aatcaacata agtatcgccc gcttgcacga gtgttgagcc cgaccagtcc atattattat
    35761 cgacgtgtac tactctgcac tgcgggtaaa tgtcgtcacc acttctgaga aaagcaataa
    35821 tgaccgattt atcgttcggg ttatttgcat acgaaccaac agcagggtaa gcataatcat
    35881 aactacccga cagaccaaaa tctgttgatt ctgctgtctg agacttcaca ttaatccggt
    35941 gataatctat tgcactccag ccattgtcaa tacttgtatt gtgcactaca tgaatggtgc
    36001 cattcagata aaatccgctc aatattctgc aatcgcccac gtcgagcaat tcgcttgtac
    36061 catattggcc ggcattgcca cctacactgt attcctttac tgttatgggg tagctgtaca
    36121 aggtcggatt gccacttgaa taaggcttgt caatatacca cagacgcaat ttgtcagagc
    36181 ctgaagaccg attgcttacc aaatacatac cgggaccata gtttcccttc aaaccgtacg
    36241 acaaaggaaa caaagtaaag gctccaaaag gttcagaact caggttggta taataaaaat
    36301 atttgatcgg gtcttttgca taaacagaac ttttatcaat ctgcattagc attactctgc
    36361 tgaatttccc agcagaatca tataggttgc ccgtgatgaa aatatcatta gctgacatac
    36421 ccagattagg ataatcgaac cataggttac tgtttgtaag atctcctttg attgtataat
    36481 agtaccaacc atcttcctta gggttgtcag aataggatat agccaacagc accttcgata
    36541 catcgggttt gcttccatgt agcaatacca tgacaaatct gttgctttca ctgtcaaaca
    36601 atatgcgtgg gtcatacttt ttacttgata actcagaagc agaataaaaa tcggaccagt
    36661 attcgccccc aagataacta cctgctgtat tgtagtattc aatgccatcg ttatttaggg
    36721 ctactatatt gcctgtagtg gataccgcga atgacaaatc tggtggtgta cctttcacca
    36781 tccacgggcc ctcgaatttc aacagtattt ccggatcttc agcagtacgc gcattttcag
    36841 tctgtgctgc gatagttttt ttcttattct gggttaaata ctcctttatc ttactaactt
    36901 caggatcact at
//
LOCUS       DAOQQY010000005        31749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_410_length_31749_cov_6.377579, whole genome shotgun sequence.
ACCESSION   DAOQQY010000005 DAOQQY010000000
VERSION     DAOQQY010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 31749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31749
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_410_length_31749_cov_6.377579"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="PLE23_00655"
     CDS             <1..374
                     /locus_tag="PLE23_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HMX81803.1"
                     /translation="KPGTIEEWLDCGNKDSLVNANQRILEFNKTKDLTDSTCVIENSV
                     IIQPCFIGKNAKIHNSVVGPYVSIGEDSVLEHVVISNSIIQNKSILRYGVVQDSMVGS
                     SAAYESTAMNLNIGDYSTLKN"
     gene            377..2077
                     /locus_tag="PLE23_00660"
     CDS             377..2077
                     /locus_tag="PLE23_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX81804.1"
                     /translation="MLKYFFTYCSVIFLCFQSVGQTDQITEKESQLQQKFIEAKTEVL
                     MTRYDQAEKKFLEIIKENETIGAVHFELGKVYEAKGDNERAIGSYNRAITLEKNNKWY
                     YLQKLYLLKKLNAYTEAATVCEQLTKVDPTSADYYYDWATYALYANNPQLALTALDKL
                     EEKQGPNLNIAEKKIRIYQNLNQADNIKSTIEKLLVIAPSCVPCLKLQARYFADVNQP
                     KEATEVWKKILTLDPADTEAKISIAAGLKSAGDDLGFLESIKPLFVNNNVSYDTKMKE
                     LVPYIQKLAKTKDPKLGEALVTALNMLEQTNPDNAKTYAAMADVAQNTGKFKEAAELY
                     KKCLQLDKNVFDVWEQRLYILDQIRDYPQMLKTSEELLDLYPGFALAAYWNGLALMRN
                     GKADDALSILSEGLMSARNNPANQYKLQILKAMAFARKNDIAKSQEAFKAARELNPEI
                     DWAYFAEARVIADIPEKLKDAIKLCDQAIKINPDNFEAIATKAYVLSKNNETDKARAL
                     YEESLAKGGIDYPEILEEYGDFLHRLNDNNAALRYWKIAIDKGTPLPVLRKKIDEVKL
                     "
     gene            2061..2858
                     /locus_tag="PLE23_00665"
     CDS             2061..2858
                     /locus_tag="PLE23_00665"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HMX81805.1"
                     /translation="MKSNYNFIVRTFVLAVMISLLSACAIFKKNAIPAEEIKPESPEK
                     IETSALLNLKGNFDEMNSFQAKTNVEVFFQGNNQEVKSQIRWVRDSVIWMNFSLFGIE
                     GARLLINRDSFFLLDRINKKYMAEQLSQLATKQNLPVSFDNLQAILLGFPVLLSEERI
                     IEDPKPEFVHFIQQDSTWRGDYFTSKDKRELTKMNLRQKKSKNSVTTELSEYKRIRSY
                     GKFAHNRVIEFYSKQTGQAKLNIIFKDITINIPKEIKFSIPSSYEKM"
     gene            2845..4125
                     /locus_tag="PLE23_00670"
     CDS             2845..4125
                     /locus_tag="PLE23_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMX81806.1"
                     /translation="MKKCNPLLNLYGLIAMLMIFVTHLSAQSKAQLESRREELLREIE
                     LTSQKIEKARSDKSDAAQRIQTLETQIEKRNELVNNLCESIDLLNNQAIQTNSQATIL
                     QKELDQLKYDYRQVMQTAYRRHLNYHYYLMLFSARSVNDFFNWVLYIKQFDTFVTEKV
                     KSITDNQQALMDNIYKIESSKEQKLKLVSEQERQQNKMTSEINEKNSLVQQLSEQEST
                     LIRKIRDKENEKEQINRKIEKIIAAEIKAAKEAELKKTKSSPKSDSKRSGKTSEKTAN
                     TSPKSTPKADPLSVNFEGNKGKLPWPLKKAIVIAGFGKKEHPTVKGIYTNNKGIYIKT
                     DQAAEVTSVFDGKVVAILSSPAYKECVLVKHGDYFTLYANLNEVNVKKNSSVSAGQVL
                     GRVAFEDTYNSPVLYFEIWKGESPMNPELWLKSL"
     gene            4151..5362
                     /gene="hflX"
                     /locus_tag="PLE23_00675"
     CDS             4151..5362
                     /gene="hflX"
                     /locus_tag="PLE23_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMX81807.1"
                     /translation="MSKDWNTGKHQQSNLKKEDEKAVLVGLVTQHQSDEKVQEYLDEL
                     EFLALTAGAVTTKRFVQKLERPDTRYFVGKGKMEEIARYVDEHQIDLVIFDDDLSGKQ
                     QHLIEEVLKIKVVDRSTLILDIFASRAQTAQAKTQVELAQLQYLLPRLRGMWTHLERQ
                     RGGIGMRGPGEKEIETDRRIVRDIISGLKKDLEKIERQAHTQRKTRGEMIRVALVGYT
                     NVGKSTLMNLLSKSEVFAENKLFATLDTTVRKVVFGTMPFLLSDTVGFIRKLPHHLVE
                     SFKSTLDEVRESDILLHLVDFSHPQYEDHIATVNQTLQDIDCRDKPTLLVFNKIDAYR
                     ERNFDEMLDAETKANIEQELTENLRNKYDSDAILISAITGENIPTLKDKIAEMVEKQY
                     MIRYPYKTNNW"
     gene            5365..6462
                     /locus_tag="PLE23_00680"
     CDS             5365..6462
                     /locus_tag="PLE23_00680"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HMX81808.1"
                     /translation="MKSIEEKYARLLTSYCLELKEGDKFYLKSTTLAEPLVREVYKCA
                     IEAGAIVEVDLDFAEKNNIFLHTAKEHQLRYVSPLYYNAMDKFDAYLLIRAPFNLKED
                     ASVPAEATKIRSEALAAANRRYFTRIADRSLKRSLCQYPTQASAQEAGMSLTEYSDFV
                     YRACKLHLDSPEEGWLKVRAEQQRIVDHLNKCQRVHYKGPDTDIRFSTKGRTWINSDG
                     RSNMPSGEVFTSPVENSVEGTIRFSFPAIHGGHEVEQATLWVKKGIIYKWEASKGKDY
                     LDKIFNIPGTTRFGEAAIGTNYEIQKITKNILFDEKIGGSIHMAIGQSYLQCGGKNKS
                     SVHWDMISDMKQGGEIWADDELIYKDGQFLI"
     gene            complement(6511..7809)
                     /locus_tag="PLE23_00685"
     CDS             complement(6511..7809)
                     /locus_tag="PLE23_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX81809.1"
                     /translation="MTDRRELNNQKVINAWAIFDWANSAYALVITTAIFPKYFLAVTD
                     PVIQVGGLSITNSSMYAYSISLAYIIIALCSPALSGIADSSGRKKLFLRTFTTLGATA
                     CIALFYFKGMNQLYLGAGAFILATIGFSGGLVFYNSYLPEIATEDQYDRVSARGFAYG
                     YVGSVILLLINLTIILKPEMFGITTAGLPARIAFVMVGMWWIGFSQITFKYLPKDRKT
                     QLNAGLLTRGFGEIKRVWAELKTMRNTRLFLLAFFCYSAGVQTVIYMASTFADVELKF
                     PASELIALIIILQLVAIGGAYLFAHLSKLRGNKFSLITMLVIWISICGGAYLVYEKWQ
                     FYLISIAVGMVMGGIQALSRSTYSKLLPDDLHDTTSFFSFYDVLEKLAIVAGTFSFGF
                     IENLTGGMRNSVLALGMFFVVGILLLMNVRINNTVVSARS"
     gene            8514..9428
                     /locus_tag="PLE23_00690"
     CDS             8514..9428
                     /locus_tag="PLE23_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81810.1"
                     /translation="MKAIKTLTLMFFMVVSLALKAQTPTDAFTMEKGQLCPVLAYQYD
                     TWEKYWEGSLFRINKNLGTVTSHTVMPMFALGLNNRFTVIASFPWMQKATSAGTLNKE
                     KGWQDLSLWLKALVFKQNLGEKNSVDAFASVGYTAPLGGYYADYLPLALGSGSNQLNT
                     RLILTWRHSSGIFASVMGGYDFRSNIDIDRTNYYIDQHYLTNELYMPNVISASITAGY
                     FKNLSRVELKYNIMDTDGGQDIRRNDQPYPSNNMDNGRVELFVQQRMPFYDPLGVIAS
                     VAYTTSGRNVPQALSYGVGLQMLIQLWK"
     gene            9463..10998
                     /locus_tag="PLE23_00695"
     CDS             9463..10998
                     /locus_tag="PLE23_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMX81811.1"
                     /translation="MKNIKFLIVAFALIAVVYACKKDVQAEPDIQAFTYDNVDENAGN
                     WKPIYIADKESIVVDAPKDVNSPEYQKELADLKAATASLTSAQKAAVEYWGANSIVRW
                     NQIAQELAAKYNLPAAENAEGVYPVPNASNPTNYPLFPFANPPYASRAFAYWTTAQFD
                     AMIIAWKKKYEFKRPAPFVTDPSIVTSLPKQSIPSYPSEDAVVAQIAQDILTFMFPGE
                     ADFLAKKAAEHKQSRIWAGMNVQSDIAAGEQIAKQVSASYIQRAKTDQMKIAGSSPSK
                     TDSLRNSAIQRLGAAWVSLEIPARPGMLPLYGKVKPWCIPSVEAVRPAPPPKVGSPQF
                     NKDREEIINYINHPTAETRRIANYWSDGPSSSTPPGHWNDIAAQLIVKNKLNPIRTAR
                     VFAYMNTAVADAGISCWDAKYYYFTERPSTSVVNLKTWIGVPNFPSYTSGHSTFSAAG
                     ATVLGHFFPSEAARMNEIATEASNSRIYGGIHFRHDCEAGLASGKEIGNYSVQLAQQD
                     GGE"
     gene            complement(11125..13275)
                     /gene="rnr"
                     /locus_tag="PLE23_00700"
     CDS             complement(11125..13275)
                     /gene="rnr"
                     /locus_tag="PLE23_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMX81812.1"
                     /translation="MQNKRNTPGEKLSSHTLQKAITDLLAKNPGKKFSPGLIKKILKV
                     DNNSDSIKYALEKAIEKLKSPTKKSVGKNSSQNTTERKAEYYEGIVDMTRSGAAYIIC
                     DSFGEDIYVQPKYIQTALDRDKVQVRLFKGGRRNRAEGEIVKILERNTTRFICTLLVR
                     AHKAIARPEQIVLPFDIEIDAESLKSAGHKDKVVIEIYKWPAKGFQSALARITQNLGK
                     EGGINLAMNSILINNGFNIDFPEEVKEEAIRLSEKLTKEDMSTRRDFRNVLTFTIDPE
                     DAKDFDDALSYRILDDGNVEIGVHIADVSYYVTPGTALDKEAYFRSTSVYLADRSCPM
                     LPETISNELCSLRPNEDKRTYSAVFTFDKKNKIIDRWFGRTLIHSDKRFSYEQAQQIL
                     DSKEGELAKEILAMNEVAISLRKERYKKGSINFESDEVRFKFDEAGHPVSVFIKERHE
                     ANLLIEDFMLLANREVATFIHNKAKGQEIPFVYRVHDLPSLEKLEQLSLFAKELGFDF
                     NASTPDKIKDSFNRLAKASATDDALKLLEPLAIRTMAKAEYTTVNIGHYGLAFDFYTH
                     FTSPIRRYSDVLSHRILEKNLTGIYREDKEVLEAQCKHISSMERHAMDAERQSVKYFQ
                     VMFLEDKVGQTFEGIVAGIIERGLFIELTDSKCEGMLPFDSLYESFDVEPSRLKATGK
                     RTGRIFKMGDRLKVKVMDTDLEARQINFELEQEQ"
     gene            complement(13275..13781)
                     /locus_tag="PLE23_00705"
     CDS             complement(13275..13781)
                     /locus_tag="PLE23_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81813.1"
                     /translation="MELTEQLDFSAFLERFPIIELPVQIDSESFHDVNSVNEPLPNIL
                     VSAYLQRTGEDEDLAEYVPVFRLPGGETFVALVYWRAGLLKYDYLVATYTLNGGLIDQ
                     TTLASTIYSEEKIIKSIAKFDEEGFVTVIEGVENAKTHELSAAESKQLFFEILDNGTI
                     QQSNKLSI"
     gene            13913..14605
                     /locus_tag="PLE23_00710"
     CDS             13913..14605
                     /locus_tag="PLE23_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HMX81814.1"
                     /translation="MNWFQSFLVSSIGQKIIMSLTGIFLMLFLVVHLVGNLQLLHDDG
                     GQAFNLYTYFMTHNPLIKTVSYLLYITVLIHAIQGILITRKNQASAGGMNRYVKKSTY
                     TTTLANRYMAFWGILIFIFLVIHLKDFWYVMKFTSDLEMVTYPGETHEVKNLYQRVAV
                     EFKELWVVILYVACMVALAFHLAHGFWSAFQTVGASHPKYLRLLKTTGKIFAFLIPLG
                     FALIPVLFYLFK"
     gene            14634..16550
                     /locus_tag="PLE23_00715"
     CDS             14634..16550
                     /locus_tag="PLE23_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HMX81815.1"
                     /translation="MKLDSKIPEGHMGQKWENYKEHARLVNPANKRKLDIIVIGTGLA
                     GASAAATLAELGYKVKAFCFQDSPRRAHSIAAQGGINSAKNYKNDGDSVYRLFYDTVK
                     GGDFRSREANVHRLAEVSLNIIDQCTAQGVPFAREYGGLLDNRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALERQIGLGNVKMYNRHEMLDIVTIDGKARGIIAKDLISGKLERHF
                     GHAVLLCSGGYGNVFFLSTNAMGSNVTAAWRAYKRGAYFGNPCYTQIHPTCIPVSGEY
                     QSKLTLMSESLRNDGRVWVPKKKEDCGKHPNEIKEEDRDYYLERKYPSFGNLCPRDIA
                     SRAAKEVCDEGRGVSASGRAVYLDFADAINRLGLDVVKARYGELFPMYQNITGHDPLK
                     QPMMIYPAVHYTMGGLWVDYELMTSVPGLYALGEANFSDHGANRLGASALMQGLADGY
                     FVIPYTIGNYLAGEIFTKAIPTDHPAFEEAEKQVRDRLNKLMNAKGDTPVDEYHKRLG
                     KIMWEKCGMARHAEGLKEARKEIQELRTEYWKNVRVPGDINEFNPELDKAMRVADFME
                     LGELMVMDALNRNESCGGHFRVEYQDNGEAKRNDEEYSYVAAWEFKGDNTEPELHKET
                     LNFEEVQLAVRSYK"
     gene            16591..17343
                     /locus_tag="PLE23_00720"
     CDS             16591..17343
                     /locus_tag="PLE23_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HMX81816.1"
                     /translation="MKINLKVWRQKNENTAGKMVDYSVNEVSPDMSILEMVDVLNDDL
                     IRKGDDPIAFDHDCREGICGMCSMVVDGRPHGPQHGATTCQLYMRNYKDGDTIYLEPF
                     RAHAFPVVKDLVVNRGAFDRIIAAGGFISVSTGQAQDANNLPVEKAVAERAMDVAACI
                     GCGACVAACPNGSAMLFVSAKVSHLNQLPQGKPEASRRAINMVKQMDAEGFGRCSNVG
                     ACEAECPKEISITNIARLNREYLKAVFTSTEE"
     gene            complement(17460..19100)
                     /locus_tag="PLE23_00725"
     CDS             complement(17460..19100)
                     /locus_tag="PLE23_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81817.1"
                     /translation="MKLSVLVGVLLLFALQLTATNYYSDPVNGSMSNSGAGTSPWGSL
                     SDIFTANKKFEVGDTIFLRTGNHGYAVIKGVNTGFVVIMPEAGHSPIISRVRISSTAN
                     SPAKFWKLSGLTIQSQATGNNATPSYYLLEVYPYATDITISGCTITSDLNTAGWTRDD
                     WRNRCNSGIFTRPRLNANILIENNLILNTAFGLAVGSSHTIVRNNTVQYFTNDGSRVL
                     GSHIIFEHNRIMDLMKVMTKDENHDDLFQSFTNANGVGQDTLRDNIIRANLFLCASDT
                     SRPFRGAVQGIGCFDGPFLNWTIENNIVICDHWHGISMYGAIDCKIINNTVIDPYVYT
                     PFDPYDNNPTSVGPAWIRIDKKNGGPGSINNTVNNNLVAASVIIVSATMGAASNNIVI
                     GAVGNYSSFFVNADDVANPSNFDLHLKGSASAVDAGISDFAPLEDYDGNIRPKGASFD
                     VGAFEYDPTTSLVQNLNDDLAFACFPNPSTSHLTIESKCNQKIDRLQLCTLQGRLVLS
                     KNINDFNAELNLSGLSDSVYFLKIYCGNVSSSRLIIVH"
     gene            complement(19695..20282)
                     /locus_tag="PLE23_00730"
     CDS             complement(19695..20282)
                     /locus_tag="PLE23_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMX81818.1"
                     /translation="MRIIAFLLVLLGSITACKEKSAEPGEMPTLPSSSIFNLSSQWEN
                     QNGDTLHLTDLQGKTLVVVMIYSSCKTACPILIAKMKQIESKFDRKDIDKISMVLVSI
                     DPETDTPEHLKAFAAKNGMDGNQWIFLRSDENATREFANVLAMKYKKISPIDFSHSNI
                     ISVFKPNGELVKQEEGEINIDNVVTTVNKVVKGNS"
     gene            complement(20297..21142)
                     /locus_tag="PLE23_00735"
     CDS             complement(20297..21142)
                     /locus_tag="PLE23_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HMX81819.1"
                     /translation="MKKYFGLVFIALLIACTKEQVTKQVMNDKIPQQLPDTLRKLNVN
                     TKMAYIPGGEYKPFYSSDTGLVTVHPFLMDERPITNSEFLAFVKANPQWQRSKIKQIY
                     ADSSYLRTWLSDTTFAADALPDAPVCNVSWYAANAFAQSVNKRLPKLDEWEFVAMADR
                     TSKDARKKPEYSDAIIDLYLQKDRQFQPVAKSTPNYWGVYNMFDVIWEWTEDFNSVLT
                     TGESRGGKYEDKSLFCAGSATTSTDLLNYAAFMRFSLRSSVKAHYTIANLGFRCARDT
                     MIVKK"
     gene            complement(21161..22222)
                     /gene="nirK"
                     /locus_tag="PLE23_00740"
     CDS             complement(21161..22222)
                     /gene="nirK"
                     /locus_tag="PLE23_00740"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HMX81820.1"
                     /translation="MKLRFLTLSAIAMMTGLFACKMADNKQSGTSSDAVNIVAEGTPT
                     VAELTAAPKVPAPVGTRAAKKLIVNLETTEIEGVIDDTIPYTFWTFNGTVPGSFIRVR
                     EGDEVTLNLKNAANSVLPHNIDLHAVTGPGGGAEATTAAPGQEASFTFKAINPGLFVY
                     HCAAPPVPMHIGNGMYGLILVEPAGGLKPVDKEYYIMQGDFYTKASTVKKGLLEFNND
                     KAVAENPDYVLFNGKKGSLLGNNMIEAKVGETVRLFVGNGGPNMTSSFHVIGEIFDNV
                     YIEGGSTVSHNIQTTMIPAGGSAIVEFKCDVPGEYVLVDHSLSRAFNKGCIGKLKVTG
                     DANPKVFKKGGAEKQKSAY"
     gene            complement(22244..23611)
                     /locus_tag="PLE23_00745"
     CDS             complement(22244..23611)
                     /locus_tag="PLE23_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HMX81821.1"
                     /translation="MKSNISTVSFYKSRVCILFLLFGISLSSSAQHSTDTLHNTLTVK
                     AQLRTRGEYRHGAFRPLNKNEDPAALISERIRLSFEYKYKDQMQVKIVPQSVGIWGQE
                     AMVQGAESSGNRLAFFEAWAMLKIAKGFDLKVGRQVISLDDERFLGEVDWAQGARAHD
                     AVALLYKKSRFELNTYLAFNQNYRNLYNNNLSNPAGNVFSTAGGFPYKHMQSIWASIP
                     VSESAKVTLLVNNIGLQDAQSATDDSAINNVSTIGANYFVSKEKFSGGISAYYQFAEI
                     KNAPKISAYLIAANASFKAGNGWTLGAGSDLISGNDVGGSITVNHSFTPYFATGHKFF
                     GSMDYFYAGNGHKGVGVSDTYLNVGKKFAKGTDIRVAFHQFITPNVVSEGSGKDYAGN
                     LGQELDLTGSMKINTFASVTGGYSVYFNTKTIDYLKSVTNTGQVQHWVWLSLNINPLL
                     FNTKF"
     gene            complement(23733..24509)
                     /locus_tag="PLE23_00750"
     CDS             complement(23733..24509)
                     /locus_tag="PLE23_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX81822.1"
                     /translation="MDNLTRFKQQIGKYRLEGCKLGEGSDLAICLPGFSLSGEWMARH
                     YPALWTGKCCYLLDWPGLGDESLDSTPFDINIISAWVKMLVHDKKPDNIWLVCHSFGA
                     RVACRLLAENPFAGHLILIAPVTEMNLRESFIRVIPSKLYNVLVRYMLSKKRLGYILN
                     LAKKTGIINVQEYSFLSEFSKNDKLNEYLIHYALVIPRLEVSTLMMNKLLGFLYECRI
                     FLFQNDKLCNNKYWQNIAKEHTNIEIKSLKGNHFTRIIKV"
     gene            24871..25593
                     /locus_tag="PLE23_00755"
     CDS             24871..25593
                     /locus_tag="PLE23_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81823.1"
                     /translation="MTTFSFYQSKLSNHAKFVSMIAVCLSFIISGCIKRQDPKIQLEK
                     IYREYDVKSFTTDVEIFPFEDDYTKICIKNPANDNILISLNVSFISEETLSLKSCKIS
                     YQKDFIIIEPNDSRNIIVYNFNEKLQNLENVKGIEYHKIHGIGRYTFGKDAIPFVQYV
                     NNKKTNPTHSSMNSRESGDGGPKISCKNGGCGATECTYKSGQIASTGGAEGGVTCGTG
                     YFACCNATDDCKCIDNKKGGCN"
     gene            25903..27321
                     /locus_tag="PLE23_00760"
     CDS             25903..27321
                     /locus_tag="PLE23_00760"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMX81824.1"
                     /translation="MLFNSAEFAIFLPIVLLVFFGLPHRFRWQWLLFSSCVFYMAYIP
                     WFILVIGTTIVIDYFAGIFIAGQHGKMRKYGLIISLIANIGILFFFKYFNFFYDNLVP
                     VLSSGGIHISKPNWNILLPIGLSFHTFQAMSYTIEVYRGHQAAERHFGIYALYVLFFP
                     QMVAGPIERPQNMLHQFHEEKFFSEAKLVSGLRLILWGLFKKLVIADRAAIVSDEVFA
                     HPVQYGSIGIFWASVMFAFRIYGDFSGYTDIARGAARMLGFELMLNFRRPYLSASVTE
                     FWRRWHISLSTWFKDYVYIPLGGNRALVSRVVFNLLITFIVSGLWHGADWKFVVWGGL
                     NGAYIAAEYLWTKLNLPPMSGRAGTNFNRIKTFLLICISWIFFAAPNMQLAIQAIRQL
                     RVISHRTTDISDVLLAKDSLMASVFIFFLLFCEILIEKERLSAWFFSQARWFRWMSYA
                     TVCWIILLFGQFGERQFIYFVF"
     gene            27321..28217
                     /locus_tag="PLE23_00765"
     CDS             27321..28217
                     /locus_tag="PLE23_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81825.1"
                     /translation="MKHSLFKILIFIGLNALLLVMALLLVHWIEPKYERTNTTTEATF
                     SAIPKNHKYDFVIMGTSHAREFSRSANHEMVEQLTGKTFFNLSKGAGHGGLIPNIMAW
                     RLFLQRGNKTQHIIYFVDPWIFYSSKWNEENYCFEDEPVRPDILMLGITNGMSTATII
                     NYLKSKLKPSYLFAPQILSALNEKFLTKADVHLIQKQIDRNFPDALSEDNFKKYSAIF
                     TNFVNQLTQAGIRITFVLPPTLLTNEPGHKQTLQLLHSFANIPVYDHSKLITNPAFYY
                     DHSHLNTAGIRSYVTENPFIFR"
     gene            complement(28234..29058)
                     /locus_tag="PLE23_00770"
     CDS             complement(28234..29058)
                     /locus_tag="PLE23_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMX81826.1"
                     /translation="MKRILVPTDFSKCAGKAMSYALEIALHTKAEILCLHSISPYEGV
                     DVDGSGLLWIREYQEAKAKTLNTWVKKFKRDARFKELNITTKCNIGFTVTDIKESAKS
                     FNADLIVMGTTGATGVGGMLFGSVAGGVISATTTPTFLVPVSAKFDILNNFALATDFQ
                     NQCSKESIEVMNDLLNAHKQSTLKVVHVLPEKAKNPPTQKEKDMQQKMKGINLEFTYM
                     HDENVPRAINNFLDVSKTKVLCVISHHHGYLHKLFKGSTTKKLAFQAHTPMLVLFD"
     gene            complement(29089..29499)
                     /locus_tag="PLE23_00775"
     CDS             complement(29089..29499)
                     /locus_tag="PLE23_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81827.1"
                     /translation="MKLKITQSTTIGEVKKHFTDAYPFLKLEFFSKPHDAYKTSPAKF
                     IVHDETKSLLEVGMSEVVEAELNIEGSTSVKSFEAEGMKYGLNLQVLRRSASTWLATT
                     VTDNLSLHEQNRKGEASTFDHSAPKDDFDYREQE"
     gene            30061..31245
                     /locus_tag="PLE23_00780"
     CDS             30061..31245
                     /locus_tag="PLE23_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81828.1"
                     /translation="MAKSLTYNIFVAILLIAQLPSFGQKTNPLKFDLSNGSTFTLSQY
                     SGNNYPKYISVGVNSSLVQGTFNQNLKTDAQYTNSNSPGRWRDEGLKTMSYRGGSDIL
                     QQGCFQFRADATGRENIKVEWTVRVISFNTNTNCIELQWKDGNDVGGTWNDVDDDLFV
                     QGTTPDPTSFSVILPPAANDLSDLRIRWIYYEKGTGIRDRLAIDDVVISSSPKPLPVD
                     LSRFDARNENKKAVLTWQTQSELDCKSFNIEKSFSGKDFSQIGEVEGNGTTNIVHEYR
                     FQDPEQLGKIQYYRLKQIDYSGEFSYSPIVSLQGEPSKAISVYPTLAKNELTISIDDK
                     NTEEYSITIIDLNGQIQSSQTIIPGSHKIIIPVNMLAEGNYILNVASQQTQHTFKFVK
                     TK"
     gene            31306..>31749
                     /locus_tag="PLE23_00785"
     CDS             31306..>31749
                     /locus_tag="PLE23_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81829.1"
                     /translation="MRFILLLGILLTAITTYSQQRVEGSLLLGGAYYGGDLNQKLIAL
                     DYLYPCYGATLRYNFKPGIAAKFQFLKGTIAGDDEKTPNKARGLHFKSPFFAIGIAGE
                     IIPWRKYRFNNMGIFQKSFSPYASVGVSMIQSADEVQSRFPQAGQI"
BASE COUNT         9791 a         6731 c         6195 g         9032 t
ORIGIN      
        1 tcaaaccggg taccatcgaa gaatggctcg actgcggcaa taaagattca ctcgtcaatg
       61 ctaaccaacg catcctcgaa tttaacaaaa caaaggacct tacggattct acgtgtgtta
      121 tagaaaattc ggtcatcatc caaccatgct tcattggaaa aaacgcaaaa attcacaact
      181 cggtagtagg gccttacgta agtattggtg aagattctgt gctagaacat gtcgttatca
      241 gcaacagcat catacaaaac aagtcaattt tgcgttatgg tgtagtacaa gattcgatgg
      301 taggtagttc ggcagcgtat gaatctacag ctatgaacct taacattggc gattattcaa
      361 ctttaaaaaa ctgagattgt taaaatattt tttcacatat tgtagcgtta ttttcctttg
      421 ctttcaatcc gtagggcaga cagatcagat aaccgaaaag gagtcgcagc ttcaacagaa
      481 gtttattgaa gccaagaccg aagtgttgat gacacgctac gaccaggctg aaaaaaaatt
      541 ccttgaaatc atcaaggaaa atgaaaccat aggcgctgtt cattttgagt tgggcaaagt
      601 gtatgaggca aaaggtgaca acgaacgtgc aattggttcg tataaccgtg ccattacact
      661 tgaaaaaaac aataagtggt attacctcca aaaactctac cttctaaaaa aactaaatgc
      721 ctatacagaa gctgctaccg tgtgcgaaca gctgaccaag gttgacccca cttcagctga
      781 ttattattat gattgggcca cttatgcttt atatgccaat aatccacaac ttgctttgac
      841 tgcccttgac aagctcgagg aaaaacaagg accaaacctg aacattgcgg agaaaaaaat
      901 tagaatttac caaaacctca accaagctga taatattaaa tcaactatcg aaaaactcct
      961 tgttatagca ccgtcgtgtg tgccatgcct taagctgcaa gcaaggtatt ttgccgatgt
     1021 caaccagccc aaagaagcta cagaagtgtg gaaaaagata ctcacactag atcctgccga
     1081 cactgaggct aaaatatcca ttgccgccgg gctgaaatct gcaggagacg acctaggctt
     1141 ccttgaatca atcaagcctt tgtttgtaaa taacaatgtc agctacgata caaaaatgaa
     1201 agaactggtg ccttatatac aaaaactggc caaaacaaaa gacccaaaac tgggtgaagc
     1261 acttgtcaca gctctgaata tgctggagca aaccaatccc gacaatgcaa aaacctatgc
     1321 agcaatggcg gatgtggcac aaaataccgg aaaattcaaa gaagcagctg aattgtataa
     1381 aaaatgtctc caactcgaca aaaacgtgtt tgatgtgtgg gaacaaagat tgtacatact
     1441 cgatcagata cgtgactatc cgcaaatgct gaaaacctcc gaagaactgc tcgacctgta
     1501 ccctggtttt gcacttgcag catactggaa cggactggca ttaatgcgca atggcaaagc
     1561 ggatgatgca ctgtccattt taagtgaagg gctgatgagc gcaagaaata atcctgccaa
     1621 ccagtacaaa ctccagatac taaaagcaat ggcattcgcc cgaaaaaatg atattgccaa
     1681 aagtcaggaa gcattcaagg cagcacgtga gctgaatcct gagatagact gggcatattt
     1741 tgccgaagca agagtaattg ccgatatacc tgaaaaattg aaggatgcca taaaactctg
     1801 tgatcaggca ataaaaatta atccggacaa cttcgaggca atcgccacaa aagcttatgt
     1861 gctcagcaaa aacaacgaaa cagataaagc acgtgcgctc tacgaagaat cgcttgccaa
     1921 aggtggaata gattatcctg aaatactgga agaatacggt gactttctcc acaggctgaa
     1981 cgacaacaat gcggcactcc gatattggaa gattgcgatt gataaaggaa ctccattgcc
     2041 tgtactcaga aaaaagatag atgaagtcaa actataattt cattgtgcgg acatttgtgc
     2101 tggccgtcat gatttcatta ctgtctgctt gcgccatttt caagaaaaat gctatccctg
     2161 ctgaagaaat aaaaccagag tctcccgaga aaattgaaac ctctgccttg cttaatctga
     2221 agggaaattt tgatgagatg aattcttttc aggctaaaac caatgtggaa gttttttttc
     2281 agggcaacaa tcaggaagta aaatcacaga taaggtgggt acgcgatagt gtcatctgga
     2341 tgaatttcag tctttttgga atcgaaggcg ccagattact catcaaccgc gattcgtttt
     2401 ttctgttaga ccggatcaac aaaaaatata tggctgagca gcttagtcag ctggctacca
     2461 aacaaaacct tcctgtatca tttgataatt tacaagcgat tttactgggg ttcccggtac
     2521 ttttatcaga agaacgaatt atagaagatc ctaaacctga gttcgtacat ttcatccaac
     2581 aagatagtac atggcgcggt gactatttta caagcaaaga caaaagagaa ctcacaaaaa
     2641 tgaatctccg tcaaaagaaa agcaagaatt ctgttactac agaattatca gaatataaaa
     2701 gaatacgcag ttatggtaaa tttgcccaca atcgcgtcat cgaattttat tcaaaacaga
     2761 ccggccaggc caaactgaat attattttca aagacattac aatcaatatt ccgaaagaaa
     2821 tcaaattttc aataccttca agttatgaaa aaatgtaatc ctcttctgaa tctgtatggt
     2881 ttgatagcaa tgctgatgat ttttgtcacg catctttctg cccagtccaa agcgcagctg
     2941 gagtcaaggc gtgaagaatt gcttagagaa atagaactta cctctcagaa aatcgaaaaa
     3001 gcacgttctg acaaatctga cgctgctcaa agaattcaaa cactcgaaac ccagatagaa
     3061 aaaagaaacg aacttgtcaa taatctctgc gaatctatcg atctgctaaa taatcaggct
     3121 atacaaacaa actcgcaagc taccatatta cagaaagaac tcgaccagct caaatacgat
     3181 taccggcagg tgatgcagac cgcttacagg cgccacctca actaccatta ctacctgatg
     3241 ttgttttcgg cacgttctgt aaacgacttt tttaactggg ttttgtacat caaacagttt
     3301 gacacattcg tcaccgaaaa agtaaagtcc atcaccgaca accaacaggc actcatggac
     3361 aatatttaca aaattgaatc ttcaaaggaa caaaaattaa aactggtaag cgaacaggaa
     3421 cgccaacaaa ataaaatgac ttctgaaatt aacgagaaga acagtttggt tcaacagttg
     3481 tccgaacagg aatcaacact cataagaaag attcgcgaca aggaaaacga aaaagaacaa
     3541 ataaacagga aaatagaaaa aatcattgcc gcagaaataa aagctgctaa ggaagctgaa
     3601 ctgaaaaaaa caaaatcatc gcccaaatct gatagcaaaa ggtctggcaa aacatctgaa
     3661 aaaactgcaa atacttcacc caaatctaca cctaaggctg accctctttc tgtaaatttt
     3721 gaaggtaata aaggaaaact gccctggcca ttaaaaaaag caattgtcat tgcaggtttt
     3781 ggcaagaaag aacatccaac tgtcaaaggc atttatacaa acaataaagg aatatatata
     3841 aaaaccgacc aggctgccga agtaacatct gtattcgacg gaaaagtagt agccatacta
     3901 agctctccgg catataagga atgtgttctt gtcaaacacg gcgattattt cacgctgtat
     3961 gccaacctca acgaagtaaa tgttaaaaag aacagctccg tgtcggccgg gcaggtatta
     4021 ggcagagtag cttttgaaga tacatacaac agccctgttt tgtatttcga aatatggaaa
     4081 ggcgagtcgc ccatgaatcc ggaactttgg ttaaaatcat tataataact attaactatc
     4141 agataataac gtgtcaaaag actggaatac cggaaaacat cagcaaagta atcttaaaaa
     4201 agaggacgaa aaagccgtac tcgtaggttt ggttacacaa catcagtcgg acgaaaaggt
     4261 acaggaatac ctcgacgagc tagagtttct ggcgctgact gccggtgccg tcaccaccaa
     4321 aagattcgtc caaaaacttg aaagaccaga tacaagatat ttcgtaggga aaggaaaaat
     4381 ggaagagata gcccgttatg ttgatgaaca ccaaatcgac ctggtaatct ttgatgatga
     4441 cttgtcgggc aaacaacaac acctcatcga agaagtgttg aagataaaag tcgttgatag
     4501 aagtacactc atcctggata tttttgcttc acgcgctcag acagctcagg caaaaaccca
     4561 ggtcgagctt gcacaactcc aatatctgct gccccgcttg cgtggtatgt ggacccacct
     4621 cgaacgtcag cgtggtggta tcggtatgcg ggggccaggt gaaaaggaga tagaaacaga
     4681 ccgccgaata gtacgcgaca tcatctcagg cctgaaaaaa gatctcgaaa aaatagaaag
     4741 acaggcacac acacaacgca agacccgtgg cgaaatgata agagtagcac tcgtaggtta
     4801 taccaacgta ggaaaatcaa cgctgatgaa cctgttgagc aaatcggaag tattcgcaga
     4861 aaacaaactg tttgccacac ttgataccac agtgaggaag gtagtgttcg gcactatgcc
     4921 ttttttatta tcagatacag tcgggtttat acgcaaactg ccccatcacc tcgtagagag
     4981 cttcaagtca acactcgacg aagtcagaga aagtgatata ctgctacatt tggtagattt
     5041 ttcacatcca caatacgaag accacatagc cacagtcaac caaacattgc aagacattga
     5101 ttgtcgcgat aagcctactt tgcttgtttt taataaaata gatgcctacc gcgaacgcaa
     5161 ctttgacgaa atgctggatg cagaaaccaa agcaaacatc gaacaagaac taactgaaaa
     5221 tctccgcaac aaatacgatt cggatgcaat cctgatatct gctattactg gagaaaacat
     5281 accgacactc aaagacaaga tagcagaaat ggtcgaaaaa caatatatga tacgttaccc
     5341 atacaaaacc aataactggt aatcatgaaa agcattgaag aaaaatacgc cagactactc
     5401 acttcttatt gccttgagct gaaggaaggc gacaaatttt atcttaagtc aaccacactc
     5461 gcagagccac tcgtaagaga agtgtacaaa tgtgccatcg aagccggtgc aatagtagaa
     5521 gtggatcttg attttgccga aaaaaacaat attttcctcc atacggccaa agaacaccag
     5581 ctcaggtacg tgtcgccctt gtactataat gcaatggaca aatttgatgc ttatctgctg
     5641 atacgagcac cgtttaacct caaagaagat gccagtgtac cagccgaagc tacaaaaata
     5701 agatcggaag cacttgcagc agccaataga aggtatttca cccgcatagc cgaccgctca
     5761 ctcaaacgaa gcctttgtca atatccgact caggcatcgg cccaggaagc gggcatgtct
     5821 cttacagaat acagcgattt tgtttaccgt gcctgcaaac tacatctcga cagtccggaa
     5881 gaaggctggc tgaaagtaag agcagagcag cagcgtatag tagatcatct caacaaatgc
     5941 caacgtgtac actacaaagg cccggacaca gacatccgct tctcaaccaa aggccgtacc
     6001 tggattaact ccgacggaag aagtaacatg ccaagcggtg aagtctttac ttcacctgtt
     6061 gaaaattcag ttgaaggaac aatccgattt agctttccgg ctatacatgg cggtcatgag
     6121 gtagagcaag ctacactttg ggttaaaaaa ggcattatat ataaatggga agcatcaaaa
     6181 ggaaaagatt atcttgacaa aatatttaac atacccggta ccacacgttt tggcgaagca
     6241 gccatcggta ccaattatga gatacagaag ataacaaaaa atatcctctt cgatgaaaaa
     6301 ataggtggct ccatacacat ggccattggc caatcgtact tgcaatgtgg tggcaaaaat
     6361 aaaagttctg tacactggga catgatttca gacatgaaac aaggcggcga aatatgggct
     6421 gatgacgaac ttatttacaa ggatggtcaa ttcctgatct gaagctgacc aataattctt
     6481 taactctgta agtcgtcgta tcaccatcat ttacgacctg gcagacacaa ccgtattgtt
     6541 tattcttaca ttcattagca agagtatgcc caccacaaaa aacatcccta gagcaagcac
     6601 actgttacgc ataccgcctg tcagattctc aataaagccg aatgaaaatg tacctgccac
     6661 aatggcgagt ttttcaagta catcataaaa gctgaaaaaa ctcgtggtgt catgtaggtc
     6721 atcgggtagc agcttggagt aagtagatcg tgataaagcc tgaatgccac ccatcaccat
     6781 gcctaccgct atggatataa gataaaactg ccatttttca taaaccagat atgcaccacc
     6841 acatatcgag atccagatga cgagcatggt tatcagactg aatttgttcc ctctgagttt
     6901 tgacaaatga gcaaacagat aagcaccacc aatggctacc aactgtaata tgatgatcag
     6961 agcaattagt tcggaagccg gaaacttaag ttcgacatct gcaaaggtag atgccatata
     7021 aataaccgtc tgtacacctg cactgtaaca gaaaaaagca agtagaaaca atcttgtgtt
     7081 gcgcattgtt ttcaattcag cccacactct tttgatctcc ccaaatcctc ttgtaagcaa
     7141 gcctgcgttc aattgggtct ttctgtcttt tggcaaatat ttgaacgtta tctgcgaaaa
     7201 cccgatccac cacataccaa ccattacaaa tgcaattcgt gcaggcaacc cggccgtagt
     7261 gataccaaac atctccggct tgaggatgat ggtcagatta atgagcaaca atataacgct
     7321 gcctacataa ccataggcaa aacccctcgc acttacgcga tcgtactggt cctcagttgc
     7381 aatctcaggc aggtaagaat tataaaaaac gagcccgccc gaaaagccga tggtagcaag
     7441 gatgaaagcc ccggcaccca gatacagctg attcattccc ttaaagtaaa ataaggctat
     7501 acatgctgta gcgcctaagg tggtgaatgt tcggagaaaa agctttttcc gtccactcga
     7561 atcagctatg cccgacaaag ccggcgaaca aagagcaatg atgatgtatg caagcgaaat
     7621 agaataagcg tacatcgaac tattggtaat actcaaacct ccaacttgaa tcaccggatc
     7681 ggtaactgcc agaaaatact taggaaaaat agccgtagtt ataaccagcg cataggccga
     7741 gtttgcccaa tcgaatattg cccaagcatt gattaccttc tgattattga gttctcttct
     7801 gtctgtcaaa atgatttttc tttagcagca aggtaagcac aattattatt ttgttgcgat
     7861 ttcttgagaa attattgata aaatttcttc taagcaattt ttaatattta ccaataattt
     7921 gaattaatat ttataaaagc attaccctca atccgaatac tatatatcat catgaaattt
     7981 ggatcaattc cttaccaaat atacctgttt tacgcagctt atcaatatat taaataaaac
     8041 aataacaact aagcgcttag ttatcagtta gttgtttttg atacacttaa ttttatgcaa
     8101 atcttgtttt tacaatattt tatttcgata cattgagagt aatacaacgc aaataatcaa
     8161 aataccttat tagaaatgta ttaaattatt ttagcaatac tgaatgtagt gaattttggc
     8221 gatagttttg cattacgaca aattatatat ttcaaaatat cacacatgag aagaacctcc
     8281 tacatatcca tttcagctaa aatagcccca gaatgtaagc ttggcttgtt aaaatctaag
     8341 acaaattaca tcaacggcaa cctggtcggt aaatgaccaa aaccacactt gtgaaatgtg
     8401 tgtatggtta cttctttata cgagtgattt tttaaaacaa aaagcagcaa atacttaata
     8461 ttcaataaca aacaaaccat tattttctaa ttcattaaaa acaaacaccg cagatgaaag
     8521 ctattaaaac acttacgtta atgttcttca tggttgtgag cttagcactt aaagctcaga
     8581 cgcctacaga tgcatttaca atggaaaaag gccaattgtg cccggtactg gcctatcagt
     8641 atgatacctg ggaaaaatac tgggaaggca gtctgttcag aatcaacaag aatctgggta
     8701 ctgtcacatc acatactgta atgccgatgt ttgcgttagg gctcaataac cgctttacag
     8761 ttattgcttc gtttccgtgg atgcaaaaag ctacctcagc aggtacgctc aacaaggaaa
     8821 aaggttggca agatttgtca ctttggttaa aagcgcttgt ttttaaacaa aatctgggcg
     8881 aaaaaaactc tgttgacgca tttgcatcgg taggttatac tgcaccgctt ggtggctatt
     8941 atgccgatta tctgccactg gctttgggtt ctggttctaa ccagctcaac acacgcctta
     9001 ttcttacctg gagacacagc tcaggcattt ttgccagcgt aatgggtggt tatgacttca
     9061 gaagcaatat tgatattgac cgaaccaact actacattga ccaacattat ctgacaaacg
     9121 aactttacat gccaaatgta atctctgcca gtataacagc agggtatttt aaaaacctat
     9181 caagggtaga attgaaatac aacatcatgg atactgatgg aggtcaggac atcagacgca
     9241 atgaccaacc ttacccttcc aacaacatgg ataatggtcg ggtagagctt tttgtacaac
     9301 agagaatgcc attctacgat ccgcttggtg ttattgcaag tgttgcttat actacttcag
     9361 gacgcaacgt acctcaggct ctttcatacg gagttggtct gcagatgctt atccaattat
     9421 ggaaataata accaattagc taataccaaa aataataata aaatgaaaaa tattaaattc
     9481 cttatcgtcg cattcgcctt aatagctgta gtgtatgcat gtaaaaaaga tgtacaggca
     9541 gaaccggata ttcaagcatt cacttatgac aatgttgatg aaaatgcagg caactggaaa
     9601 ccgatttata ttgccgataa agaaagtatc gtagtcgatg ctccaaaaga tgtaaattcg
     9661 cctgagtatc aaaaagaact ggctgactta aaagcagcaa cagcttcact aacatctgct
     9721 caaaaagcag ccgtcgaata ttggggcgct aactctatcg ttagatggaa tcagatagcc
     9781 caagagttgg cagcgaaata caaccttcct gctgcagaaa atgcagaagg tgtatatccg
     9841 gtaccaaatg catctaaccc aaccaattat ccactgttcc catttgcaaa tccaccttat
     9901 gcaagccgcg catttgcata ctggaccact gcacagtttg atgctatgat catcgcatgg
     9961 aagaagaaat acgaattcaa aagaccggca cctttcgtaa cagatccatc catcgttacg
    10021 agcctgccta agcaaagcat cccttcttac ccatccgaag atgcagtagt agctcagatt
    10081 gctcaggaca tcctgacatt tatgttcccg ggtgaagcag attttcttgc caaaaaagcc
    10141 gctgagcaca agcaaagccg tatatgggca ggtatgaatg tgcaaagtga tattgccgcc
    10201 ggcgaacaaa ttgccaaaca agtaagcgca tcatacatcc agagagcaaa gaccgaccaa
    10261 atgaaaattg ctggaagttc tccttccaaa accgactcac ttcgcaacag cgccatccaa
    10321 agactaggtg ctgcatgggt aagccttgag ataccagcaa gaccaggtat gcttccactc
    10381 tacggtaaag taaagccttg gtgcatacca agtgtagaag cggtacgccc ggcaccacct
    10441 cccaaagtag gctcacctca attcaacaaa gacagagaag aaatcatcaa ttatatcaac
    10501 cacccaactg cagaaacacg ccgaatagcc aactactggt ctgacggacc aagctcttca
    10561 acgcctccgg ggcactggaa tgacattgcc gctcaattga tcgttaaaaa caaacttaac
    10621 ccaatccgaa cagccagagt gtttgcttat atgaatactg ctgttgctga tgccggtata
    10681 agctgctggg atgcaaagta ttattacttc accgaacgtc cttcgacttc agtagttaat
    10741 ctgaaaacat ggataggtgt acctaacttc ccaagttaca cttcaggcca ctctactttc
    10801 tcggctgcgg gcgctactgt tcttgggcat ttcttccctt cagaagctgc cagaatgaac
    10861 gaaattgcta ctgaagcctc caacagccgt atttacggtg gtatccactt cagacacgac
    10921 tgtgaagccg gacttgcttc tggtaaagaa atcggaaact attcggtaca gcttgcacaa
    10981 caagacggcg gtgaataatc acatcaattg ctaatctctt tttaaactat agaggctgtc
    11041 atcactggca gccttttttt tatttcccct caacggggaa tatgccataa tgcataatga
    11101 gctttaaacc tagacaacgc aggtttactg ctcttgttca agttcgaagt taatctgtct
    11161 tgcctccaga tcggtgtcca tgacttttac tttcagcctg tcacccattt taaatattcg
    11221 accggttcgc ttacctgttg ccttcaatct tgatggctct acatcaaatg attcgtagag
    11281 gctgtcaaac ggcaacatgc cttcacattt gctatctgta agttcgataa ataaccctcg
    11341 ctctatgata cctgctacta ttccttcaaa tgtctgacca actttatctt caaggaacat
    11401 cacctgaaag tatttgaccg actggcgttc agcatccatg gcatgacgct ccatagaaga
    11461 tatgtgcttg cattgtgctt ccaatacttc cttgtcttct cgatagattc ctgtcaggtt
    11521 tttttcaaga atacgatgtg acaatacatc agaatatcgc cttatcggtg aagtgaaatg
    11581 tgtgtaaaag tcaaaagcaa gaccataatg cccgatattg acagtagtat attcggcctt
    11641 ggccatagtg cgaatggcta atggctccag cagcttaagt gcatcatcag tagctgaagc
    11701 tttagcaagc cggttaaacg aatccttgat cttgtcgggt gtgctggcat taaaatcgaa
    11761 accgagctct ttggcgaaca acgacagttg ttcaagcttt tctaaggaag gcagatcgtg
    11821 tacacgataa acgaaaggta tctcctgccc ttttgcctta ttgtgtatga atgtagctac
    11881 ttcacggtta gcgagcagca tgaaatcttc tatcaaaagg ttggcctcgt gccgttcctt
    11941 gataaaaacc gaaaccgggt gccctgcttc atcaaattta aacctcactt catccgattc
    12001 gaagttgatt gaacctttct tgtaacgttc ctttctgagt gatatggcta cttcattcat
    12061 ggctaaaatc tcctttgcca actcaccttc tttactgtcg agtatctgct gtgcctgttc
    12121 gtaagaaaat ctcttgtcgg agtgtataag ggttcgacca aaccaacggt caatgatttt
    12181 gttctttttg tcaaatgtaa atactgcaga gtaggttcgt ttatcttcat tcggtctgag
    12241 cgagcacagt tcgttggata tggtttcagg caacatggga caactcctgt cagccagata
    12301 aacactcgtt gatctgaagt aagcctcctt gtcgagtgca gtgcccggtg ttacataata
    12361 agagacatcg gctatgtgta cacctatctc tacattgcca tcatcgagta ttctgtacga
    12421 aagcgcatcg tcaaagtctt tggcatcctc aggatcaatg gtgaaagtaa gaacatttcg
    12481 gaagtcgcgc ctggtactca tatcttcttt tgtaagcttt tcgcttagtc tgatcgcttc
    12541 ttcctttact tcctccggaa aatcaatatt gaacccatta ttaatcagga tcgaattcat
    12601 ggcaaggttt ataccaccct cttttccaag attctgggtt attctcgcca atgctgactg
    12661 aaaaccctta gcaggccatt tatagatttc tattacaacc ttatctttat gacctgctga
    12721 tttgagtgac tcagcatcta tttcgatatc aaaaggcaac actatttgct caggtcgtgc
    12781 tatagcttta tgggctctta cgagcaatgt gcaaataaac cgtgttgtgt tgcgttctag
    12841 tatttttact atttctcctt ctgctctgtt gcgcctgcca cctttaaata accttacctg
    12901 taccttgtcg cgatctagcg ctgtttgtat atatttaggc tggacataga tatcttcgcc
    12961 aaatgagtcg cagattatat atgctgcacc ggagcgtgtc atgtccacta ttccttcata
    13021 atactcagct tttcgttctg ttgtgttttg tgagctattt tttcctacag atttctttgt
    13081 cggtgatttt agtttttcaa tagccttttc gagtgcatac tttattgaat cactattatt
    13141 atctactttc agaattttct ttatcaatcc tggtgaaaat ttctttcctg gatttttggc
    13201 caagagatca gttatcgcct tttgaagagt atgagaactt agtttttcac caggagtatt
    13261 tcttttattt tgcattaaat gcttaattta tttgattgtt gaatagtgcc gttgtctaat
    13321 atttcaaaaa ataattgctt gctctcagcg gcagagagtt catgtgtctt tgcattttct
    13381 acaccttcta taacggtgac aaaaccttct tcgtcaaatt ttgctataga ttttattatt
    13441 ttctcttcag aatatatggt gcttgccagt gttgtctggt ctatcaaacc gccgttaagt
    13501 gtataggtcg ctacaagata atcgtacttg agcagaccgg cccgccaata tacgagggct
    13561 acgaaagttt ctccgcctgg caatctgaac accggaacat actctgccag atcttcatct
    13621 tctcccgtcc tttgcaaata ggcagatacc agaatattgg gaagaggttc attgacagaa
    13681 ttaacatcat gaaatgattc ggaatcaatc tgtacaggta gctcaataat cgggaatctc
    13741 tctaaaaatg cactaaaatc aagttgttct gtaagctcca tttgtttttt tctctaatat
    13801 tttttatttt agaataattc taaatagctg aaaatgttaa gaaatacggc gcaatatgtg
    13861 tttattaaag ccgtaaccaa taaatttgca gctccaaaat taattctaaa ttatgaattg
    13921 gtttcaatca tttttggttt cttctatcgg acagaagata atcatgagcc tcacaggcat
    13981 tttcctgatg ttgtttttag tggtacatct tgttggtaat ttgcaactac tgcacgatga
    14041 cggtggtcag gcatttaacc tatacacgta tttcatgacg cacaatccgc tcatcaaaac
    14101 tgtatcatac ttgttataca taactgtact cattcatgcc attcagggaa tactcattac
    14161 ccgaaaaaat caggcatcag ccggcggaat gaacagatat gttaaaaaat caacctatac
    14221 taccacgctt gctaacagat acatggcatt ctggggcata cttatcttta tcttcctggt
    14281 catccacctg aaagattttt ggtatgtgat gaaattcacc tctgacctcg aaatggtcac
    14341 ttatcctggt gaaacacacg aggtaaaaaa cctctaccag cgcgtagcag ttgagtttaa
    14401 agaactctgg gtggtaatat tgtatgtggc ctgcatggtt gcgcttgcct tccacctggc
    14461 tcatggtttt tggtcagcat tccaaacagt aggtgcaagc cacccaaaat atcttagact
    14521 gctgaagaca acaggaaaaa tatttgcctt cctgattccg ctcggttttg cacttattcc
    14581 tgttttgttt tatttgttta aatgatccgg tacggtagat aaaataaatt tttatgaaac
    14641 tagattcgaa aattcctgaa ggccacatgg gccaaaaatg ggaaaattat aaagaacatg
    14701 caaggcttgt caatcctgcc aataaaagaa aactcgatat catcgtcatt ggtaccggac
    14761 ttgccggagc atccgctgct gccacattag ccgagctcgg ctacaaagta aaagcattct
    14821 gttttcagga tagcccacgt agggcacact ctattgccgc acaaggcggt attaactcgg
    14881 ccaagaatta taaaaatgat ggtgactcag tttatcggct gttctatgat actgtaaaag
    14941 gtggagactt ccgttctcgt gaagccaatg ttcaccgtct ggctgaagtg agtcttaaca
    15001 tcatcgacca atgtactgca cagggtgttc cgtttgctag agaatatggc ggattgctcg
    15061 acaaccgttc ctttggtggc gctcaggtgt cacgaacatt ctatgccaga ggccagaccg
    15121 gccagcaatt attacttggt gcttaccagg cacttgaacg ccagataggt ctgggcaatg
    15181 taaaaatgta taaccgtcac gaaatgcttg atatcgttac tatagacggc aaagcacgtg
    15241 gcatcattgc caaagacttg atctccggaa aactcgaaag acacttcggc catgctgttt
    15301 tgctgtgttc aggcggttat ggcaatgttt tcttcttatc tactaatgct atgggctcta
    15361 atgttacagc tgcatggcgt gcatacaagc gaggtgccta cttcggcaat ccttgctaca
    15421 ctcagataca ccctacctgc ataccggtaa gtggcgagta tcagtcaaaa cttacactca
    15481 tgagcgaatc actccgcaat gatggtcggg tgtgggttcc taagaaaaaa gaagactgcg
    15541 gcaaacaccc gaatgagata aaagaagaag acagagatta ctaccttgaa agaaaatatc
    15601 cgtcattcgg caacctgtgt ccgagagata ttgcctccag agcagctaag gaagtatgtg
    15661 atgaaggtcg tggtgtaagt gcatccggcc gtgctgtata tctcgatttt gcagatgcca
    15721 tcaaccgact tggccttgat gtagtaaaag cacgttatgg cgagctattc ccaatgtacc
    15781 agaacattac tggtcatgat ccgctcaagc aaccaatgat gatttatcct gctgtacact
    15841 atacaatggg cggactttgg gttgactacg aacttatgac ctctgttccc ggattatatg
    15901 cactgggtga agcaaatttc tctgaccacg gagctaacag acttggcgca tcagctctta
    15961 tgcaaggtct ggcagacggc tattttgtca tcccttatac cattggaaat tatctagccg
    16021 gagaaatctt caccaaagcc ataccgaccg accatccggc ttttgaagag gctgaaaaac
    16081 aggtacgaga tagactgaac aaactgatga atgccaaagg cgatacacct gttgatgagt
    16141 atcataaacg ccttggaaaa ataatgtggg aaaaatgcgg tatggccagg cacgccgaag
    16201 gtttgaaaga agcacgtaag gaaatccagg aactgaggac agaatactgg aagaatgttc
    16261 gcgtaccggg tgacatcaac gaatttaatc cggagcttga taaagccatg cgtgtagccg
    16321 actttatgga actcggcgaa ttaatggtca tggatgccct taacagaaac gaatcatgcg
    16381 gtggccactt ccgggtcgaa tatcaagaca acggtgaagc caaacgtaat gatgaagaat
    16441 attcatacgt agcagcatgg gaatttaaag gagataatac tgaacctgaa ctccacaaag
    16501 aaacgcttaa ctttgaagaa gtacagcttg cagtaagaag ttataagtaa attgtaaaaa
    16561 tttctttaga aaacataaag actcaataag atgaaaataa atctgaaagt ctggagacaa
    16621 aaaaacgaga atacagccgg taaaatggtt gactactctg tcaacgaagt ctcaccggat
    16681 atgtccattc tcgaaatggt ggacgtactc aatgatgatc tgattagaaa aggtgacgac
    16741 ccaatagcat ttgaccacga ttgtcgtgaa ggaatatgcg gtatgtgctc aatggtagtt
    16801 gatgggcgcc ctcacggacc acaacacgga gcaactacct gccagctcta tatgcgtaat
    16861 tataaagatg gcgataccat ttatctggaa ccattcaggg cacacgcctt tcctgtagtt
    16921 aaagacctgg tggttaaccg tggtgcattc gaccgcatca ttgcagcagg tggatttata
    16981 agtgtgagca caggtcaggc acaggacgcc aacaaccttc ctgtagagaa ggcagtagca
    17041 gaaagggcaa tggatgtagc agcttgtata ggctgtggtg cctgtgtagc cgcctgcccc
    17101 aatggatcag ccatgttgtt tgtgtctgcc aaggtctctc acctcaatca actaccacaa
    17161 ggcaaacccg aagcaagcag aagagccatc aatatggtca aacaaatgga tgcagaaggc
    17221 tttggaagat gttctaacgt tggagcctgc gaagctgaat gcccgaagga gatatctata
    17281 actaatatag caagacttaa ccgcgaatac ctcaaggccg tctttactag caccgaagaa
    17341 taatccaagc tatatagtca acccaaatag ctacaggaac ctgaatattt ctgtagctat
    17401 ttattttttc ggattataca tcggctgtta tctcatgaag gcgcttgttc aaagcacggt
    17461 caatgtacta taattagcct gctgctgcta acattcccac aatatatttt tagaaaataa
    17521 actgaatcac ttagcccgga caaattaagc tctgcgttga aatcattgat attttttgac
    17581 aaaacaagcc gaccctgtag cgtacataac tggagcctgt ctattttctg attgcattta
    17641 ctttcaatgg taagatgtga agtagaaggg tttggaaaac atgcaaacgc caaatcatcg
    17701 ttcagattct gtacaaggct tgttgtcggg tcgtattcaa atgctccaac gtcaaaactc
    17761 gctcctttgg gtcttatatt accgtcataa tcctccaagg gcgcaaaatc agatataccg
    17821 gcatcaacag cagaggcgct gcccttaaga tgcaggtcaa agttacttgg atttgccaca
    17881 tcgtctgcat tcacaaaaaa cgaagaataa ttacctaccg ccccgattac tatgttattg
    17941 cttgccgctc ccattgttgc agacactatg atgactgaag cggcaaccag gttattatta
    18001 acagtattat ttatgctacc tggccctcca ttctttttat cgatgcgtat ccaggcaggc
    18061 cctacagaag taggattatt atcataaggg tcaaacggtg tatatacgta tggatcaata
    18121 acggtattat tgattatttt acaatcaata gcgccgtaca tcgatatgcc gtgccagtga
    18181 tcgcaaatga cgatgttgtt ctctatggtc cagttcagaa atggtccgtc aaaacaacca
    18241 atgccttgaa ctgcaccacg aaaaggcctt gatgtatcgc ttgcacacaa gaacaaattg
    18301 gccctgatga tattgtctct caaagtgtct tgccctacac cattagcatt ggtaaacgac
    18361 tggaacaggt cgtcgtggtt ttcatctttg gtcatcacct tcatcaggtc catgatcctg
    18421 ttgtgctcaa atatgatatg actgcccaat actctgctgc catcattcgt aaaatactgt
    18481 accgtattat tcctcactat ggtatgtgac gaacctaccg ccaaaccaaa tgctgtgttg
    18541 agtatcaaat tattctctat caatatatta gcattgagtc ttggtcgtgt aaagatacca
    18601 ctgttgcatc tgttgcgcca gtcgtctcgg gtccaccctg ctgtattcag gtcggatgtg
    18661 atggtacacc cactgatggt aatatcagta gcataaggat agacctcaag cagataatac
    18721 gacggcgtag cattattgcc ggtggcctgt gactgtattg taagtccact taatttccag
    18781 aattttgccg gagagttagc tgtacttgat attcgtaccc gactgattat gggtgaatgg
    18841 ccagcttcag gcattatgac gacaaagccg gtatttacac cttttatcac cgcataacca
    18901 tgattaccgg ttctgagaaa aatggtatct cctacttcaa atttcttatt ggctgtaaaa
    18961 atatcgctca ggctgcccca aggagatgta cctgctccgc tattggacat actaccattc
    19021 accgggtctg aataataatt ggtagcggtt aattgcagtg cgaaaagtaa taatacgcca
    19081 actagtacgc tgagcttcat gtttgagata agtttgctgt aaatttatct cttatcgaga
    19141 agaattactt gtattattct ccaattattt tcatctaata ccaaaattta acccggataa
    19201 cccattaggc atgtaaacga aaatgtttag cacaataaat ccatgaaaaa acgataaaag
    19261 tatagtgaac tattttttta acttttgaaa aagatttgaa gtaaacatta ttggcagtaa
    19321 agaaccttta atgcgttctc tgggtttaat cagggcgtgc ccctgtcggg tcaggccatc
    19381 cgctttactt cgtgcccgct tctgtccttc acgcatatct ctgcattagg tgagaactta
    19441 gttaaacaca gatagcgcat tacaaatata aatgaaaatg attattacaa ataatccatg
    19501 aaaaagcatt aaaagcaact tgaactatgg cttttgaaca tttgaaatgg atttgaacga
    19561 agtattattt gcagtaaaga acctaatgcg tggtatctgg gttaaatgta ttcctcagca
    19621 agcatcctcc atacagaata tcacaaacct ataatcgtga attgtttgat ttagttgcaa
    19681 ccaaagaaac gctatcagct atttcccttt acgaccttat tcacagtagt tactacatta
    19741 tcaatattga tttcaccttc ttcctgtttc accagctcac cgttgggctt aaatacgctg
    19801 atgatgttag aatgtgaaaa gtcgattggt gaaatcttct tgtatttcat tgccagtaca
    19861 ttggcaaatt ctcttgtagc attttcatcc gaacgaagga aaatccactg gttgccatcc
    19921 attccgtttt tagctgcaaa tgctttgagg tgctccggtg tatctgtttc aggatcaatg
    19981 gatacgagta ccatagatat tttatctata tccttccggt cgaattttga ctcgatttgc
    20041 ttcattttcg ctatcagtat agggcatgca gttttgcaac ttgaataaat cataaccacg
    20101 acaagggtct tgccctgcag gtcggtaagg tggagcgtgt caccattttg gttttcccat
    20161 tgtgagctta gattaaagat cgaactcgaa ggtagtgtag gcatttcgcc gggttcggca
    20221 gatttttctt tgcaggctgt aatactacct agcagaacca ataagaatgc aattattctc
    20281 attttaatta aaatttttat tttttaacaa tcattgtatc tcttgcacat ctgaaaccca
    20341 aattggcaat ggtataatgc gcctttacag atgaacgcaa ggagaaacgc ataaaagcgg
    20401 catagttgag caaatcggta gatgtggtag cactacctgc acagaaaagc gatttgtctt
    20461 cgtatttgcc tccccgcgat tctccggtgg tcagtactga attaaaatct tcggtccatt
    20521 cccatatcac gtcgaacatg ttatagactc cccagtagtt tggtgtactt ttcgccactg
    20581 gttgaaactg gcggtccttt tgcagatata aatcaataat ggcatcagaa tattccggtt
    20641 tttttcgtgc atcttttgat gtacggtcgg ccatagctac aaactcccac tcatcaagtt
    20701 taggcaggcg tttgtttaca ctttgtgcaa aagcattggc tgcataccac gaaacattgc
    20761 ataccggtgc atcgggcagt gcatctgctg caaaggtagt atccgacaac caagttctaa
    20821 gataagacga atcggcgtat atctgcttga tttttgacct ctgccactgt gggtttgctt
    20881 tgacgaatgc aaggaactca ctgttggtga taggtctttc atccattaaa aaaggatgga
    20941 ctgttaccag gccggtatct gagctataaa atggcttata ctccccaccg ggaatataag
    21001 ccatctttgt atttacattg agctttcgta atgtgtcagg taattgctga ggtatcttgt
    21061 cattcattac ttgctttgtc acctgttctt tagtacaggc tatcaacaat gcaataaaga
    21121 ccaaaccaaa atactttttc attcctgaca ttatacaaaa ttaataagca gatttttgtt
    21181 tttcagcacc tcccttcttg aatacttttg ggttggcatc accagttact tttagcttcc
    21241 cgatacaacc tttgttgaat gctcttgata atgagtggtc aaccaatacg tattctccag
    21301 gtacatcaca tttaaattca acaatagctg aaccgcctgc aggaatcatc gttgtttgga
    21361 tgttgtgaga cacagtagaa ccaccctcta tgtaaacatt gtcaaatatt tcaccaataa
    21421 cgtggaatga agatgtcatg tttgggcctc cgttacctac aaacaaacga acagtttcgc
    21481 ctactttcgc ttcaatcatg ttattaccaa gtagtgagcc ttttttaccg ttaaacaaaa
    21541 cataatccgg attttcggca acagccttat cattgttaaa ttcaagcaag ccctttttaa
    21601 cagttgatgc ttttgtataa aaatcacctt gcatgatgta atattcctta tcaacaggct
    21661 tgaggccacc ggcaggttct accaagatga ggccgtacat accattacct atatgcatcg
    21721 gcactggtgg tgcagcacaa tgatatacaa acaagcccgg attgatggcc ttaaaggtga
    21781 aagatgcctc ctgacccggt gcagcagttg tagcttcggc gcctccgccc ggacctgtca
    21841 cagcatgtag gtcgatgttg tgaggcaata cactgttggc agcattcttc aggttgaggg
    21901 ttacttcatc gccttctctt acccggataa aacttcccgg cactgttccg ttaaatgtcc
    21961 agaatgtgta aggaatggtg tcgtcaataa caccttctat ttcggtagtt tcaagattga
    22021 caatcagttt tttggctgct cttgtaccta ccggtgccgg cactttaggt gctgccgtca
    22081 gttctgcaac agttggagtt ccttcagcaa cgatattgac agcatcagaa gaagtgcccg
    22141 actgtttgtt gtcagccatt ttacatgcaa ataagcctgt catcatggct attgcgctta
    22201 gtgttaaaaa acgtagtttc attgtgtgag attttatatt gatttaaaat ttagtgttaa
    22261 ataataacgg attgatattg agcgaaagcc aaacccaatg ttgtacttgc cctgtgttgg
    22321 tgacagattt gagataatcg atggtttttg tattgaaata taccgaatat ccacctgtaa
    22381 cacttgcaaa tgtattgatc ttcatacttc ctgttaggtc cagttcctga ccaagattac
    22441 cggcataatc tttacctgac ccttctgaca ctacatttgg cgtaataaac tgatggaatg
    22501 caactctgat atccgttcct ttagcgaatt ttttacctac attcaaatat gtatcagata
    22561 ctcccactcc tttatgtcca ttaccggcat aaaaataatc catacttccg aaaaacttat
    22621 gtccggtggc aaaatatggt gtaaatgaat gattgacagt aatacttcct ccgacatcat
    22681 taccgctaat caggtcactt cctgctccga gtgtccaacc atttcctgct ttaaatgaag
    22741 catttgccgc tatcagatat gcacttatct ttggtgcatt cttaatctcc gcaaactgat
    22801 aataagccga aattcctcct gaaaactttt ccttactgac aaaataattg gctcctattg
    22861 tgctgacgtt gtttatagct gaatcgtcag tagctgattg tgcatcttgt agaccgatgt
    22921 tgttcaccag taacgttacc tttgcacttt cactgacagg aatggatgcc caaatactct
    22981 gcatgtgttt gtaaggaaat ccacctgccg tactaaacac attacctgcc ggattgctca
    23041 gattgttgtt atacagattc ctgtaattct ggttaaatgc cagataagta ttcaattcaa
    23101 atctgctttt cttgtaaagc agagctactg catcatgcgc tcttgctccc tgtgcccagt
    23161 caacttcgcc taaaaatctt tcgtcatcaa gcgaaataac ctgacgccct accttgaggt
    23221 caaatccttt agctattttg agcattgccc atgcttcaaa aaatgccagc ctattgccac
    23281 tgctctcagc accttgcacc atagcctcct gcccccaaat gccaacagac tgtggtacaa
    23341 tcttcacctg catttggtct ttatacttat attcgaagct tagtcggata cgctccgaga
    23401 tgagtgcggc cggatcttca tttttattca atggcctgaa tgccccgtgg cgatactctc
    23461 ctcttgtacg cagttgcgct ttgactgtca gtgtgttgtg taaagtgtca gtagaatgtt
    23521 gggctgatga ggaaagagaa attccgaata acaagaataa aatacaaact ctactcttgt
    23581 aaaaactaac tgtacttata ttacttttca taagcttttt gattaaagct aaaataatgg
    23641 tacaaagaga gccggtttct aactaatcta ctatgatagc agtcatagaa tagaatgatt
    23701 ctaattagtt tatatagttc agaccaccat acctacactt taattattct cgtaaaatgg
    23761 ttgcctttca atgattttat ctcaatattt gtatgttctt tagcaatgtt ttgccagtat
    23821 ttattgttac aaagcttgtc gttctggaag aggaatatac ggcattcata caaaaaaccc
    23881 aataatttat tcatcataag ggtgctcact tcaagtctgg gtatgaccag tgcataatgt
    23941 atcagatatt cattcaactt gtcgtttttg gaaaattcac taaggaaaga atactcctga
    24001 acattgatta tgccggtttt ttttgccaag ttaagtatgt agcccagtct tttcttacta
    24061 agcatatacc gtacaagtac attgtacaat ttacttggaa taacccgaat gaaactttcg
    24121 cgcagattca tttctgtaac aggtgctatc agtataaggt gaccggcgaa tggattttct
    24181 gccagtagtc ggcatgcaac cctggcacca aaactgtgac acaccagcca aatattgtcg
    24241 ggctttttat catgtaccag catttttacc catgccgaaa tgatgttgat atcgaagggt
    24301 gtactgtcaa ggctttcatc acccagaccg ggccaatcaa gaagataaca gcattttcct
    24361 gtccacaatg caggataatg tcttgccatc cactcacccg ataatgaaaa cccgggcaga
    24421 catatcgcaa gatcgctgcc ttcaccaagc ttacagcctt cgagccggta tttgccaatt
    24481 tgttgtttga atcttgttag gttatccaat ccatttttgc ttaaggaaac ataaaattaa
    24541 acaaaaatta cgccttgcta tactcaaata tatgcaccaa atgtaataat caatcaaata
    24601 acccagataa actatattca ataagtgagc atcgtttaac tccttcattc tttgtatcat
    24661 taatacttct gagcatttga tcaccatcat tttgccttat ttgtcaaaaa taccaaggaa
    24721 aacatgccaa aacctgccat ctttcattaa caaatgttaa aatttaatca ctatttaaat
    24781 ttctcttgat tgttagcatg aatgatatta tctttgttat aatgaagtaa tcaaaaaacg
    24841 ggcggcaacc tttaattcgg catcgataca atgacaacat tttcattcta ccagtctaag
    24901 ttaagcaatc atgctaagtt tgttagtatg attgctgttt gtttatcttt tatcattagt
    24961 ggttgtataa agcggcaaga tccaaaaatc caactggaaa aaatttatcg tgaatacgat
    25021 gtaaaaagtt ttaccacaga tgttgagata ttcccatttg aagatgatta tactaaaata
    25081 tgtataaaaa accctgctaa tgataatatt cttataagtc taaatgtttc ttttatatct
    25141 gaagaaacgt tgagtttaaa atcatgcaaa atttcttatc aaaaagactt cattattatt
    25201 gagccaaatg attcacgcaa tataatcgta tataatttca acgaaaaact tcaaaatctt
    25261 gaaaatgtaa agggtatcga ataccacaaa attcacggca ttggcagata tacttttgga
    25321 aaagatgcca ttccgtttgt acaatatgtt aataataaaa aaactaaccc aacccattca
    25381 tcaatgaact cgcgtgaatc aggcgacgga ggtccaaaaa tatcgtgtaa aaatggtggg
    25441 tgtggtgcaa cagaatgtac ttataagagt ggtcagattg catcaacagg aggtgccgaa
    25501 ggaggtgtta catgtggtac aggatatttt gcgtgttgta atgcaacgga tgattgtaag
    25561 tgtattgaca ataagaaagg tgggtgtaat taacatcaaa gtaaacacac attaagaaag
    25621 cagaatctgt taagtagatt ctgctttctt attaaatgcg tctaatgaag gattaaatca
    25681 tcatttatat aatttgttag ttataattgt tataccttta ttttcacgga taacgcatta
    25741 ggattttaac cgaaaacgat cagtacaaaa agtccataaa aaagtgataa aagcatagtg
    25801 aactatggtt tttaagcttt tgaaatggat ttgaagtaat cattattggt agtcaagagt
    25861 ctaatgcgtg atctggggat tattcaggaa ttttgaattt gcatgctttt caactcagca
    25921 gaatttgcaa tttttttacc gattgtgctc ttggtgttct tcggtctgcc tcaccgtttt
    25981 cgttggcagt ggctcttatt ctcgagttgt gtgttttata tggcgtacat tccctggttt
    26041 atcctggtta taggcaccac catcgttatt gattatttcg ccggtatatt catcgccgga
    26101 caacatggaa aaatgcggaa atacggcttg ataataagcc tgatagccaa tatcggtatt
    26161 ctatttttct tcaaatactt caattttttc tatgacaatc ttgtaccggt actgtcatcc
    26221 ggcggcatac atatcagcaa acccaactgg aatatcctgc tgcctatagg tctgtcgttt
    26281 cacacatttc aggcaatgag ctataccatc gaagtatatc ggggacacca agctgcagaa
    26341 cgacacttcg gcatctatgc actgtatgta ttgttctttc ctcaaatggt ggccgggccc
    26401 atcgaacggc cccagaacat gctccaccaa tttcatgaag agaaattttt cagcgaggca
    26461 aagctggtaa gcgggctgag acttatattg tgggggctct tcaagaaact ggtaatagcc
    26521 gacagggcag ccatcgtgtc ggatgaagtg ttcgcacatc ctgttcaata cggcagcatc
    26581 ggcatattct gggcatctgt tatgtttgcc ttcaggatat acggcgactt ctccggttat
    26641 acagatattg cccggggtgc agcacgtatg ctcgggttcg aactcatgct caacttcagg
    26701 cggccttatt tgtcagcatc cgtcaccgaa ttctggcgca ggtggcatat ttcgctttct
    26761 acgtggttca aggattatgt ttacatacca cttggaggaa accgggcttt ggtatcaagg
    26821 gtggtcttta acctgctcat cacctttatc gtcagcggac tctggcatgg ggccgactgg
    26881 aaattcgtgg tgtggggcgg ccttaatggt gcctacatcg ctgcagaata cctgtggaca
    26941 aaattaaacc tcccacctat gagtggcagg gcaggaacaa atttcaacag aataaagaca
    27001 tttttactca tctgcatcag ctggatattt tttgcagcac ccaatatgca gcttgccata
    27061 caggccatca gacagttaag agtcatcagc catcgcacga cagacatttc ggatgtgtta
    27121 ttagccaaag attccttgat ggcatcggtt tttatttttt tcttgttgtt ttgtgaaatt
    27181 ttaatagaaa aagaaagatt gtcggcttgg tttttcagtc aggcaagatg gtttcgatgg
    27241 atgagttatg ctactgtgtg ctggataata ttgttattcg ggcagtttgg tgaaaggcaa
    27301 tttatttact ttgtattttg atgaaacatt cgctttttaa aatattgatt ttcattgggt
    27361 taaatgcctt actgctcgta atggcattat tgcttgtaca ttggatagaa ccaaaatatg
    27421 agcgaacgaa tacaacaacc gaagctacgt tttctgccat acccaaaaac cataagtacg
    27481 attttgtaat aatgggcacc tcacatgcac gcgaattttc aagaagcgca aatcatgaaa
    27541 tggtcgaaca actgaccgga aaaacattct tcaatctctc aaaaggagcc ggacacggtg
    27601 gtttgatacc caatataatg gcatggcggc tcttccttca acgcggaaac aagacacaac
    27661 acatcatata ttttgtcgat ccgtggatat tctacagcag taaatggaac gaagaaaatt
    27721 actgcttcga agacgaaccc gtcaggcccg atatcttgat gctcggtatt actaacggaa
    27781 tgagtacggc caccatcatc aattacctga aatcgaaact caagccatcc tatttgtttg
    27841 caccacagat actgtcagca ctaaatgaaa aatttctcac caaagcagat gtgcacctca
    27901 ttcaaaaaca aatagatcgc aattttcctg atgctttgtc ggaggataat ttcaaaaaat
    27961 attcagctat attcacaaac tttgttaacc aattgaccca agcaggcatt cgtatcactt
    28021 ttgtgctccc tcctacacta ctcaccaacg aacccggaca taagcagaca ctgcaattgc
    28081 tccacagctt tgccaacatc ccggtatatg accatagcaa gcttataacc aatcctgcat
    28141 tttactacga ccacagccat ctcaacacag ccggcatccg cagctatgtc actgaaaatc
    28201 cctttatatt caggtgatgt tccggaaaat tttttaatca aacaatacga gcattggtgt
    28261 atgagcctgg aatgccagtt ttttggttgt gctgcccttg aagagcttgt gcaggtagcc
    28321 gtgatggtgc gagatgacgc aaagcacttt tgtcttactt acatccagaa agttattgat
    28381 ggcacgtggt acattttcat catgcatgta ggtaaattca aggtttatcc ctttcatctt
    28441 ttgctgcata tccttttctt tctgtgttgg cggattctta gctttttcag gcaaaacgtg
    28501 aaccaccttc agagttgatt gcttatgagc attgagcaga tcgttcatta cctctatact
    28561 ttccttagag cactggttct gaaagtcggt ggcaagagca aaattattta gaatatcgaa
    28621 tttggcagat acggggacca agaatgtagg agtagtggtt gcagagatga caccaccagc
    28681 cacactgcca aaaagcatac cacccacacc cgttgctcct gtggtaccca tgacaataag
    28741 gtcagcattg aaagactttg ctgattcttt aatgtcggtg accgtgaatc cgatattaca
    28801 tttggtagta atgttcaatt ccttaaacct ggcatcacgc ttaaatttct ttacccaagt
    28861 attcagggtt ttagccttgg cttcctgata ttcgcgtatc cacagaaggc cgctaccgtc
    28921 aacgtcaacg ccttcgtatg gcgaaataga atgcaggcac agtatttcag ccttggtatg
    28981 tagtgctatt tcaagggcgt aactcattgc cttgccggca catttagaaa aatcagtagg
    29041 caccaaaatt cttttcatga tattgggttt atttaggtta aatatttttc attcctgctc
    29101 tctgtagtca aaatcatcct taggtgccga gtgatcaaat gtagaagcct cacccttgcg
    29161 gttctgttca tggagtgaaa gattatcagt aacggtagta gccagccagg tacttgctga
    29221 cctcctcagt acttgcaggt tcaaaccata tttcatgcct tcagcctcaa aactcttaac
    29281 cgatgtgctt ccttctatat tcaactccgc ttcgaccacc tccgacatgc ctacttcaag
    29341 caatgacttt gtttcgtcgt gtacaataaa cttcgccgga ctcgtcttgt atgcatcatg
    29401 aggctttgag aaaaactcaa gcttcaagaa aggataagcg tctgtgaaat gtttcttcac
    29461 ttcaccgatg gtagtggatt gggttatttt tagtttcatg tttattaatc aataatgttt
    29521 tactttatac aaatttaagc cacatagttt atttaaacta taaagttaat cattctttaa
    29581 catgatttta gtcaagttac tatcatttgg tttgtacttt ttcatttcag gaatatttat
    29641 acttgacaca atacaactaa ttactgtcat tgaatgacag cattttgaaa tattttttta
    29701 aaaaaattgt tacaaagagt caataaaatt acatcaatat aattttaata ttttttacca
    29761 atttgcctta tataccatta ttctctaaca gaatgttaca caacatacta taatttattc
    29821 aaattcatca tccatttctt cattttgagc aaattatatc acacttttga cctacataat
    29881 cattttacat aatattttac atatttaaca aacaacatat tagagagatt ggtttagacc
    29941 tgaattgcaa aatacattgt cacattaaaa gcataatgca gatactttta tcaataaaat
    30001 gaaagacttt tgcatgatta ttgcattact attcctcgaa catatcaaat gaaacattca
    30061 atggcaaagt ctttgacata taatatattt gtagcaatac tattgattgc tcaattacca
    30121 tccttcgggc aaaaaacaaa tcctctcaag tttgatcttt caaatggctc aacatttact
    30181 ctgtcacaat attctggcaa taattatcca aaatatataa gcgtcggagt caactcaagt
    30241 cttgtacaag gtacattcaa ccaaaatctg aaaacggatg cgcaatacac caattccaat
    30301 tctcctggta gatggcgtga tgaaggtctc aaaacaatga gttaccgtgg tgggtcagat
    30361 attttacagc agggatgttt ccagttcaga gctgatgcta cagggcgaga aaacataaag
    30421 gtagaatgga ccgtaagggt aatatcgttc aacacaaata ccaactgtat agagctgcaa
    30481 tggaaagacg gcaatgatgt aggcggaacc tggaatgacg tggatgacga cctctttgtt
    30541 caggggacaa cgcccgaccc aacctctttt tcagttatac tgcctccggc agccaatgat
    30601 ctgagcgatt tgagaatccg atggatttat tatgaaaaag gcacaggcat cagagacaga
    30661 cttgctattg atgatgttgt cattagttcc tctccaaaac cattgcctgt tgacttgagt
    30721 cgtttcgatg cccggaacga gaacaaaaaa gcagtactta catggcagac ccaatccgaa
    30781 ctggactgta agtcttttaa tattgaaaaa tcattttcag gcaaagactt tagtcaaata
    30841 ggagaagttg agggtaacgg tactactaat atagtccatg aatacagatt tcaagatcca
    30901 gaacaattag gcaaaatcca atattataga ttaaaacaaa tagattacag cggagaattt
    30961 agctattcgc cgattgtatc actgcaggga gagccttcta aagcaatatc ggtatatccg
    31021 acattggcaa aaaatgaact taccatttcc attgatgaca aaaacaccga agagtatagt
    31081 attacaatca ttgacctcaa tggacagata caatccagcc aaaccatcat acccggaagt
    31141 cataaaatca tcataccggt aaatatgctt gctgagggta actatatatt gaacgtagct
    31201 tcgcaacaga cacagcatac cttcaaattt gtaaaaacaa aataaatatc aacaagattt
    31261 ttgtcgggaa atggtatatc tttgcgtctt gcataactat gagaaatgag gtttattttg
    31321 ttattgggca tattattgac tgccattact acatattcgc aacagcgcgt tgaaggatca
    31381 ttgctacttg gtggcgccta ctatggtggc gacctaaacc aaaaacttat tgcacttgat
    31441 tatctgtatc catgctacgg tgctactttg agatacaatt tcaaacccgg tattgcagcg
    31501 aaatttcagt tcctgaaagg cactattgca ggagatgatg aaaaaacacc aaacaaggca
    31561 cgtgggctac acttcaagtc accttttttt gccattggca tagccggaga gattatcccg
    31621 tggcgcaaat accggttcaa caacatgggt atttttcaga aatctttttc accttatgct
    31681 tctgttggtg tatcaatgat tcagtctgct gatgaggttc agagcagatt tccgcaagcc
    31741 ggacagata
//
LOCUS       DAOQQY010000006        31134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_420_length_31134_cov_6.231764, whole genome shotgun sequence.
ACCESSION   DAOQQY010000006 DAOQQY010000000
VERSION     DAOQQY010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 31134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31134
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_31134_cov_6.231764"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLE23_00790"
     CDS             <1..397
                     /locus_tag="PLE23_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HMX81830.1"
                     /translation="PANLFFSTGIPVCILVLKKCKKFDDVLFINAVDHFEKGKRQNSL
                     LPENIDKIVNTYRNRTEETRYARRVTMDEIVKNEFNLNISRYVSTSLDEEIIDLKEVN
                     KKLVDLDKDINKARETHNKFLKELGLPPI"
     gene            564..818
                     /locus_tag="PLE23_00795"
     CDS             564..818
                     /locus_tag="PLE23_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81831.1"
                     /translation="MQHLYNNRIKTNKWRIFPPNPVQYLPSFGSYKSAVTKHAHRLGF
                     EFEWQTRFYDHIIRNDESFQKISNYINNNTANWQEDKFIN"
     gene            complement(1116..1199)
                     /locus_tag="PLE23_00800"
     tRNA            complement(1116..1199)
                     /locus_tag="PLE23_00800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1163..1165),aa:Leu,seq:gag)
     gene            complement(1600..2361)
                     /locus_tag="PLE23_00805"
     CDS             complement(1600..2361)
                     /locus_tag="PLE23_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX81832.1"
                     /translation="MIRAENIVKEFNGNLVLKGISAEFYPGKTNLIIGRSGAGKTVFL
                     KTLVGLNQPTSGKIWYGNTDLFSLDKKALRKIRMEVGMLFQGSALFDSLTTEQNIRFP
                     LDMFTNMTKKEKIDRVNYCLARVNLENANNKYPSELSGGMQKRVGIARAIVLEPKYLF
                     CDEPNSGLDPKTSILIDELIKSITVENNITTIINTHDMNSVMEIGDNIVFLHNGLVEW
                     NGNKSQVLGSNNELLQEFIFASPFLRRLRDAARKL"
     gene            complement(2358..3104)
                     /locus_tag="PLE23_00810"
     CDS             complement(2358..3104)
                     /locus_tag="PLE23_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX81833.1"
                     /translation="MFRNFFFHFGRYVQMLKLSLGKPEKFYMYWNEMLRMMNDIGIGS
                     LVIVILISVFIGAVTAIQFAYQLGGQLIPKYYIGYVVRDMTIIELAPTITGLVLAGKV
                     GSNIAAEVGAMRQKEHIDAMEIMGVNTAGYIVLPKILAALVVIPMLVAIAAFVGIVGG
                     YLSSVPTGWLTSTDYVKGLHEFFLPRNVSMMYVKATVFAFILTSVSCYQGYYVKGGNI
                     ELGEASTRAVVYSNILILLSDYVIAVLLTG"
     gene            3274..3981
                     /locus_tag="PLE23_00815"
     CDS             3274..3981
                     /locus_tag="PLE23_00815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMX81834.1"
                     /translation="MKKVIAITGATKGIGRAIAEKFAQNGYHVAVCARNKEDLKQMAI
                     YWTEHYPESEIFTFRADVNSKVQLQAFANHLLARFPVVDVVVNNAGVYFPGEVLTADE
                     GTLEKMMEVNLYSAYNFTRMLMPSLKKQPAAHIFNMCSIASILAYPNGGAYSISKFAL
                     LGFSKCLREELKDSTIKVTAILPGATWSDSWAGADLPVERLVQAADIADTVYAASQLG
                     KSAVVEEIIIRPQQGDL"
     gene            complement(4044..4787)
                     /gene="fabG"
                     /locus_tag="PLE23_00820"
     CDS             complement(4044..4787)
                     /gene="fabG"
                     /locus_tag="PLE23_00820"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HMX81835.1"
                     /translation="MKLLEGKNALITGGSRGIGAAIVKKFAEQGANVAFTYVSSATKA
                     EALRDELRALGVKAECYKSDAADFKQAGDLVAAVQTDLGPVNILINNAGITRDTLMLR
                     MSEQQWDEVIHTNLKSVFNLTKHCMKAMLRTGGSIINMSSVVGVFGNAGQANYAASKA
                     GIIGFSKSIAKEVGSRNIRTNVIAPGFIDTEMTGELDEKTKEAFLSGIPMKRYGTAEE
                     VANLCVFLGSDMSSYINGQVISICGGLNV"
     gene            4956..5228
                     /locus_tag="PLE23_00825"
     CDS             4956..5228
                     /locus_tag="PLE23_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMX81836.1"
                     /translation="MKKSSIIRLFIIGLLAVLFVLPLRSQFQLPSNEGVGYRVKQLRQ
                     SYQLTTAELAKYTGLSKQYLEGIECGKYAVGDTESRIISEYFGQEL"
     gene            complement(6180..7058)
                     /locus_tag="PLE23_00830"
     CDS             complement(6180..7058)
                     /locus_tag="PLE23_00830"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMX81837.1"
                     /translation="MKKNLFVVCILLISVNFAQAQLPFKLGFKAGLNASRFLGPAETD
                     NDGNTLEKFSNNTGFLVGVNAGFPFNNIFGARVELQYSINGGRRLFESQNAYQKFTTI
                     DSKPIVTTGYKRYNLNTFNSYIQLPVSLYAKLFKKIEISAGISPGFLISSTGTGEIKH
                     VFNGTTTLKDNTLVQELDYNYGSDKIGSIAGNPVNFNLNTEAIATRSILNAYENFTEK
                     SGGYYESFDLGALLGLAYYFNSSLYLGANMYYGLKDVSRETYDISLQKLDAGKFIKRN
                     DFDRNFALQFMLGFQF"
     gene            7147..8187
                     /gene="rlmN"
                     /locus_tag="PLE23_00835"
     CDS             7147..8187
                     /gene="rlmN"
                     /locus_tag="PLE23_00835"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMX81838.1"
                     /translation="MGNLTDIRKISEQELKAALAANDEPSFRVKQIQEWMWKKSVVSF
                     DEMTNLPKSLIQKLKEQFAFYPLVLDTAQFSMDGTIKSRFRLSDGYLIESVLIPVPED
                     DRFTVCVSCQVGCSLACTFCATGRMGRIRNLSPAEIYDQVVMVNTQCLHQYGKPLTNI
                     VYMGMGEPMLAYRNVLDSIRLITSPDGLNMSPRRITVSTAGISKMICKLADDEVKFNL
                     AVSLHAPDDVKRNTIMPINETNDLKSLIEALRYFQQKTNNRLTFEYIALRDFNLEDED
                     AENLIKIYKKVPARINIIEYNPIENAPFGHAPDDKVDEFAQKLRNAGVMVTVRKSRGK
                     DIDAACGQLANK"
     gene            complement(8220..9164)
                     /locus_tag="PLE23_00840"
     CDS             complement(8220..9164)
                     /locus_tag="PLE23_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HMX81839.1"
                     /translation="MSLLVVGTVAFDSIKTPFGSVEKVIGGAATYISWAATFYYRNVK
                     LVSVIGDDWPETEIHHLRARGVDTEGLQIRKGQKSFFWEGKYNNDMNSRDTLATDLNV
                     LADFDPILPESYKKSRYVMLGNLTPDIQLRVLEQLTARPKLIVMDTMNYWMDVAMPSL
                     KKVLSKVDLLTINDDEARQLSGEHSLVKAANAILKMGPSYLVIKKGEHGALLFYRNSI
                     FFAPALPLQDVVDPTGAGDTFAGGFIGYLAQSDNIQYNYMKRAMIYGSAMASLCVEEF
                     SLQKIQTLTHDEIVDRLNEFVKLTKFNIKLNYDSNDDE"
     gene            9249..9325
                     /locus_tag="PLE23_00845"
     tRNA            9249..9325
                     /locus_tag="PLE23_00845"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9283..9285,aa:Asn,seq:gtt)
     gene            complement(9388..10263)
                     /locus_tag="PLE23_00850"
     CDS             complement(9388..10263)
                     /locus_tag="PLE23_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81840.1"
                     /translation="MNGCTANIGDSAEFHYNPIKLDHDTNFSKGELFRIQSDQLEEAS
                     GIAASAYSPGHYYVHNDSGNPEEIYLIDSLGALKVKILLKGIKNRDWEDICTGPGPEK
                     GKNYIYLSDIGDNQYIHRKYNIYRFEEPKIPVGKFPKEFIVSDISKFTFSYPDKVSYN
                     AEAFMTDKTTGASYIITKSKYSSVYSLTLPATPLGNIIAQHITDLPIAEVTGADISAK
                     GDILIKDYTRVYLWKNIHSIPTDSLIKTIPVESSYTIEPQGEGICWDLNGKSYVTISE
                     VRHLSGQYLIRYYGK"
     gene            10601..11032
                     /locus_tag="PLE23_00855"
     CDS             10601..11032
                     /locus_tag="PLE23_00855"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMX81841.1"
                     /translation="MMKLCFIFIGFIHSVTSIGQPSANVKAFAPEELRMQMMTKSDVL
                     MLDKLLCDNLLYIHSNAVEEDKALHLANIASGKIRYRTMDIEQTAYFTKGKFTFTNGI
                     LRVNGVYDSKDFDIRLKYTAVYQKQKCRWKLLRWQSTKINN"
     gene            complement(11174..15208)
                     /locus_tag="PLE23_00860"
     CDS             complement(11174..15208)
                     /locus_tag="PLE23_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX81842.1"
                     /translation="MIKRIIPIVVLQFFAVLIFAQTPYSISFDGKAAPISLKELKCNN
                     AGTATVSLGAGVQSNDPVYLCFGDSLFIDHQGGQDLAGDPVPATPPGIGYAFYKNAPT
                     VPGQTLSAILSDPSVLNIPPAPGGLWVATGKTPSGDMYFKNNGSLQLLFNGGKPVQIW
                     FAPITLDDWANKKFEGTPAGNCVDVNINNAFPVVYLNKITYANFTSTTGMGGSFQGTF
                     IVKGGLPEYLGAQNYTINIYLKANPAIKGNITSGPAKHGSSVSYNVPQGGTYVIEISD
                     GKTCEFKSEVQFPAVTYTIGQGLGYKNDTVCIDITVDNWEKVVGQTLYFFYDTNVLEY
                     VSGKFVSDFNNSTILFNENTKGEVINLFVASDTDNGYTLPDGSKLFELCFKLIGNPLD
                     CSKIYIDELSPTEVPNTSVTDGSFNYTDAPQNFIEGEICILPQNTGLQVATTVVQPIC
                     NGSTDGSIQLVTLGGKAPYTYNWVKVGSPGVNGNGNIPALGGAGIINGLASGIYTITV
                     TDNSQPIETQTLTIQVQQPGVITPQFNIQQPTCHDANDGFIDVILTNGGTQPYSYSWS
                     TGLNGPNATSLSNIGNGSYKLTITDSNQCTAEFTNVLNKAEVIVSLASKQDIGCGGTG
                     KGSATLDITGGVAPYDFLWNDGNISGPTRTDLDAGNYSVTVTDVEGCSNTLNVTIIQV
                     SGITITGFDSVSVKCGEDSNGQLTVKTNIPTGSTIVSYSWSGPSATTNTATITNLKSG
                     KYFVTITDNNGCAAIDSATLWSPAPISLLNKTLVSPKCFGDKNGSIAVQMSGGTPPYS
                     YTWSTNPGVPTSQAVIASLGAGTYYVSVTDAKNCGPYIETIVLNDPPKITLQFLNIAA
                     PTCATGTCTGTAFADALYSDGNPGTFKFVWESAEIFTGVNASVALQLCSGWQSVTVTD
                     NNCGIIDSVLIPAAQEIEIIPAEIKNTTCNGLTDGSITVNLNGGVAPYTYNWSTGTST
                     TNILGNLAPGTYQLTITDAKGCSSISIPYIINEPPVLQAQLDPNITHGITCSSDSDGE
                     IQVFFTGGNSGVTTYTWSPNVSTTEYAGNLGAGIYSVTVTDAKGCTDSVSYTIQPPSP
                     LTAVIPTPEEPECNGFQTFVTVTSAAGGSGPDYTFSVDNGPALPIGQLVSIFAGDHLI
                     TVFDKKGCYIDEVVSISEPAPVSVNLGPDIEVELGDSIQLNPQGLPSNMATITWTPAE
                     AFNNPNTLNPYIYPTEPIEVKLTITDDKGCIGSDVLFIDVDNNRNVYIPNAFSPDADG
                     INDVFRVGTGTGVVKINYMKIFDRWGALLYEAKDIPAGDYTTGWDGKVNGQRLNPCVV
                     VYIIEVEFLDGVKLLYRGEINIIR"
     gene            complement(15340..16581)
                     /gene="hutI"
                     /locus_tag="PLE23_00865"
     CDS             complement(15340..16581)
                     /gene="hutI"
                     /locus_tag="PLE23_00865"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HMX81843.1"
                     /translation="MASNTVLLTNIRSLFQTRTDLKTPVKGKDLSVLPSINNAFLLIK
                     DGQIEDFGSMSSLPSLNVPIIDCTGQSVLPAWCDSHTHIVYDGSREAEFVDKIHGLSY
                     EDIAKRGGGILNSAARLRTATEDELFDQASARLKEVIGYGTGALEIKSGYGLSKDSEL
                     KMLRVIRRLKQSTDCLIKSTFLAAHAVPLEYKGRQAEYVSHIINDMLPEVAGEGLADY
                     MDVFCDRGFFTTEDTDRLLEAGWKYGLKPKIHANELDFSGGIQTGVKHSAISVDHLEC
                     VGEDEINALLNSTTIPTLLPSTAFFLKLEYAPARKMIDAGLPVALATDYNPGSSPSGR
                     MSFVISLSCIYMKMLPEEAINAATINGACAMEVQDTYGMIAKGYEGSIIITKNIPSLA
                     YLPYSFGSDLVDKVILKGKLQ"
     gene            complement(16633..17424)
                     /locus_tag="PLE23_00870"
     CDS             complement(16633..17424)
                     /locus_tag="PLE23_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMX81844.1"
                     /translation="MKGIIILTVCILTATAALATEPVNETLSIEADFNTATAKSKQLS
                     KAQVLIFSAKWCAPCIKMKENTLTDGAVVRFLNDNTINTVVDIDTREGFRLMSEFEVK
                     ELPSVIILDENGTEIARKKEFLSSTAMLKFVASATTNVTSKKANIDTINKAEKRNQET
                     NTTKSGTTPSVPVVNVQNQSKVPNVAANTYGVQIGVFSNIETALAETIRIQKLLADRD
                     ILILNKQNQIRLVIGVYISKESAQKIKAELKENGLDGFVKDLSSL"
     gene            17615..18634
                     /locus_tag="PLE23_00875"
     CDS             17615..18634
                     /locus_tag="PLE23_00875"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMX81845.1"
                     /translation="MNGDKKIRAAITGIQGYVPETVLSNIDLERMVETTDEWITTRTG
                     IKERRILKEPGKALSDMCTEAVNGLLRKTNTAADDIELLICATVTPDMVFPDTANIIL
                     DKVGAKNAYGYDIRAACSGFLYALYTASKLVESGAYKKVIVVGGDMMSSIIDYTDRAT
                     CVIFGDGCGAVLLEPTTDETGIVDAVLKADGAGRVHLHMKAGGSLKPASIETVMAKEH
                     YVYQEGQAVFKFAVKGMVSAIEEVLARNNMTTDDINWLVPHQANKRIISSVGDFLNIP
                     SQKVMVNIEKYGNTTSGTIPLCLWEWENQLKKGDNIMLTAFGGGFTWGALLLKWSYDG
                     SAQNR"
     gene            18711..19274
                     /gene="efp"
                     /locus_tag="PLE23_00880"
     CDS             18711..19274
                     /gene="efp"
                     /locus_tag="PLE23_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HMX81846.1"
                     /translation="MATTGDIKNGLCFVYNNDIYTVVEFQHVKPGKGAAFVRTKMKSL
                     TNGRVLENNFPSGHKIEVVRVERRKSQYLFKDEAGYNFMDNESFEQFTIPDGMIENAQ
                     LMKEGIEVEILFHAEKETALTVDLPLHVVLQVTYTEPGLKGDTATNAMKPAKLETGAE
                     IRVPLFVNEGDLIKIDTRTNSYMERAK"
     gene            19283..19786
                     /gene="accB"
                     /locus_tag="PLE23_00885"
     CDS             19283..19786
                     /gene="accB"
                     /locus_tag="PLE23_00885"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HMX81847.1"
                     /translation="MTYKEIQELINLVAKTNLSEVKLINGDFKLIIRSKEFSSGKAVQ
                     VVAPAPASQPATQVVAPPVSTEPAISTVTEKAVQPTEPTTGGNYIEIKSPMVGTFYRA
                     SGPDKPAFVQVGDNVSKGQRICIIEAMKLFNEIESEVSGKIVKVLVDDSTPVEYDQPL
                     FLVDPAG"
     gene            19834..21180
                     /gene="accC"
                     /locus_tag="PLE23_00890"
     CDS             19834..21180
                     /gene="accC"
                     /locus_tag="PLE23_00890"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMX81848.1"
                     /translation="MFNKILIANRGEIALRIIRTCKEMGIKTVAVYSTADRESLHVRF
                     ADEAVCIGPPSSTESYLNIPRIMAAVEITNADALHPGYGFLSENANFAEVCQEYGIKF
                     IGPTAAQIRKMGDKVTAKETMIKAGVPVVPGSDGLLKDTTKGKKLAKEIGYPVILKAT
                     AGGGGRGMRIVWKEEEFEDAWNSARQEAKAAFANDGIYMEKYIEEPRHIEFQIAGDQY
                     GNVCHLSERDCSIQRRHQKLVEEAPSPFMTDELRQKMGEAAVKAGKSIKYEGVGTVEF
                     LVDKYRNFYFMEMNTRIQVEHPVTEEVIDYDLIKEQIKIAAGVPISGKDYYPIMHAIE
                     CRINAEDPKNNFRPSPGLIKTLHIAKGHGVRVDTHVYTGYKVPSNYDSMIAKLIVKAR
                     TREEAITKMERALDEFIVEGIKTTIPFHKKLMRDPKFIAGDFNTAFLNDFDFSDLD"
     gene            21213..23030
                     /locus_tag="PLE23_00895"
     CDS             21213..23030
                     /locus_tag="PLE23_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX81849.1"
                     /translation="MSKLSRLLGYVKKYKYLVALNIFSNILLSLFTIATIPAIIPFLQ
                     ILFRQTPRKTIPVELKSNLSNLIEFVEWKFSYLITSVGEEKAVFFACGMLIVLFFFSN
                     LFRYAALYFISPVRYGVIGDIRESVFSKIMRLPMSYFSEERKGDLMSRVTADVQEIEV
                     SILSVLEAIFREPLMIIGALTYMLYTSPTLTLFAFGMILVIGFVVWVISKTLRKHSSE
                     AQQRLGSVTSIIEESLGGLRVIKGFNAEQYQQSKFEAENLLYKKTMVKILRKRDMASP
                     LSEWLGVTIVALLIVYGASLVFKGDMQASAFLAFLFAFFRVIDPAKSLSSAWTNIQKG
                     YAAMDRIDMIMHADEAIKDAPDALPIQTFNSAIEFRNVHFKYKNSETTVLKDINLVIP
                     AGKTVALVGASGSGKSTLLDLIPRFHDATQGTILIDGTDIKQLKIYDLRELLGIVTQD
                     AILFNDTVYNNIVFGQKDVTRQQVEDAARTANAHNFIVAMEDGYHTNVGDRGSKLSGG
                     QRQRITIARAVLKNPPVLLLDEATSALDSESEKLVQQALNYLMKNRTSLIIAHRLSTI
                     MQADEILVMSEGNIIERGTHDQLMAQNGEYAALVQLQSI"
     gene            23094..24215
                     /locus_tag="PLE23_00900"
     CDS             23094..24215
                     /locus_tag="PLE23_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81850.1"
                     /translation="MMKKFTFLIAFVCSMVNVNAQIIVTNAAWPKAGDTTKLANDSES
                     GYLLAPAQATNGNWDFSKLKEESIDLTSFAPASQGNGFADFPTAELYSKSNFADVYYN
                     VTNSAFEILGYKGDILGFGFPLVVKGKPAAVERRNPMKYFDISNTEYTFGATFSAELL
                     PDSLFSGIPFTPDSVRFGQNIKRLDVVDSWGKLKIPLGTYDVLREKRTSIQTIKLEVK
                     IPVLGWTDVSNFFGGAGGTPDTSVAYHYIANGSTEPIMVLNCTATGDTIQDIDFKTNK
                     TKVGIFSPSLSIGKLNIFPNPANHFIELNTTGIIEGKAHLMITDLLGKTMREETDILT
                     KEPYNWFINLDNFNVGMYFVSLKDEKGNIKASGTFSIVR"
     gene            24280..25227
                     /locus_tag="PLE23_00905"
     CDS             24280..25227
                     /locus_tag="PLE23_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMX81851.1"
                     /translation="MFGQFHLPTPLVRLDGFSSHHEVFMKRDDLTHPVISGNKYRKLK
                     YNLLAFKEGNYEALMTKGGYYSNHIAAAAAAGKYFDIKTIGIIRGEKPLNPGVTLNFA
                     QSAGMQLSYMSRAEFREFSYQPGHPEFENIYFLPEGGTNELALKGCGELAEELALQID
                     LNNTLICLASGTGGTTAGLADVLPENVKVLSFSALKGKWLKDEINSLLHKNVSHVDVT
                     DEYCFGGYAKADDRLFHFINNFYNKYGIPLDPVYTAKMMYGLEEMLNQERITPGKTII
                     AIHTGGLQGVFGFQGSKVPIHWYNDPFCREASALKSSML"
     gene            25393..26652
                     /locus_tag="PLE23_00910"
     CDS             25393..26652
                     /locus_tag="PLE23_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMX81852.1"
                     /translation="MSTPVIIIVMGFFIFWGHYMNGVFERRSIPDVLGLMILGLLIGP
                     ILNLVDTSTFGQFGSIFSNLVLLFILFESGTDLKFAEVKNSLRAAATITTIGFLFTWM
                     TLTPLCYFMLEIPILPSLFIGAALGGTSSAVVVGLVKKINVRPKTAATLIMESAETDV
                     FTLAIPLSILGLMTSANVQVNTVLSSFISSIILSLIIGIGGAFLWTFILGRYKDLKNT
                     KFMTPAFLFILYGITEYLHFSGPLTALSFGIALGNLDYFEPKMLERIIPNQDIALKKD
                     EKDFFSEMVFLLRTFFFVYIGLSIKIDDLFWLTWGAGFTGILFLVRIIAVKLVMHRDT
                     PVLDKGVISFMIPKGLGAAVIATLPLQEGHPYGSAIQALCFSIILFSTIYTSILFFLT
                     KRGITIPLYKIIFGKDKNSTVTNDTAM"
     gene            complement(26788..27687)
                     /locus_tag="PLE23_00915"
     CDS             complement(26788..27687)
                     /locus_tag="PLE23_00915"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMX81853.1"
                     /translation="MDELLTIDPKDLPVKDLHQHIVGSIAPRPIAFASTMDENGIPNI
                     APYSFFNAFSSNPPILVFSSNRKVADNKTKDTLHNVLATKEVVINVVNYAIARQVAIS
                     SVEYAPDVNEFEKTGLTPIPSETVKPYRVKESPVQMECSVKEVIPLGEKGGAGHLIIC
                     QVSRMHISTSILDENNRIDPQKADLVGRLGRAWYARIKGDCIFSHHQPVNIIPVGYDN
                     LPEAIRTSPVFTGHDLGVFASKTEFPDKEKIKEIKTNDSKVKDILGQPNPSMELQRYA
                     KHLIDINREDDAFCVAMISLDCH"
     gene            27731..28261
                     /locus_tag="PLE23_00920"
     CDS             27731..28261
                     /locus_tag="PLE23_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMX81854.1"
                     /translation="MQRTIITVFILLSIWAAFKLARDYYFKPNVSAGEQIPKTIVQLK
                     DGKELDLAAWKGKYVLLHFWGSWCGPCRRESPEIRTLYENFHTKQYNNADGFEILSIG
                     IEKDESRWNSAIIADRLTWDYHTTELNGFESRMAKLYGIRQIPILLLVGPDQRMIGSA
                     QTPDSVKEILTEKIKQ"
     gene            28335..28895
                     /locus_tag="PLE23_00925"
     CDS             28335..28895
                     /locus_tag="PLE23_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMX81855.1"
                     /translation="MTQKNNEGTNLHADDQEMVEVREEEHNIAEDVEQVEHPDPNESL
                     NAQIAELKDKYLRLVAEFDNFRKRTARERIELEQVASKEVMLALLPVLDDFERARKIA
                     DEEASPEPFSEGIRLVYNKLTQTLKSKGLQAMETDGANFNEEMHEAITELDLGGANTG
                     KIIDTIERGYTLNEQIIKYAKVVVGK"
     gene            28937..30109
                     /gene="dnaJ"
                     /locus_tag="PLE23_00930"
     CDS             28937..30109
                     /gene="dnaJ"
                     /locus_tag="PLE23_00930"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMX81856.1"
                     /translation="MAKRDYYEILGVDKNADADTIKKAYRKVAMQYHPDRNPGDKASE
                     EKFKEAAEAYDVLNDPDKKARYDRYGHAGVDPNMGAGGFRGGNGGMTMDDIFQNFGDV
                     FGSDNPFESFFGGGSSRGGQQKQGQRGSNLRIKVKMTLEEIAKGVSKKIKVRKQVKCT
                     SCKGSGAKDAQSTSTCSTCRGSGYVRQIKNTFLGQMQTTTTCPTCSGSGQVITAHCNT
                     CKGDGRMWEEETIEIDIPAGVEDNMQLSLRNKGNAGPKGGPSGDLLIHIEEVKHEYLT
                     RDGMNLAYELNLNFADATLGTSVEVPTINGKARVKIDPGTQAGKVLRLKGKGLPSVHN
                     AYSTGDQLIYVNIYTPKDITAEERTLLEKMRNMPNFQPKSEKSEKGFFERMKDIFS"
     gene            30129..30638
                     /locus_tag="PLE23_00935"
     CDS             30129..30638
                     /locus_tag="PLE23_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HMX81857.1"
                     /translation="MKVKVILSVGLVLLLAGCGPDYIFKDYQTIDSKGWDYNKRIDFQ
                     FEINDTSKVYNLILDVMHADTFASENAYVKVYTTFPDGKKTEQQLSLELASKTGNWFG
                     KCSSGSCNLLIGLQENVVFAQSGTYKFSLEQFGRSNPLTGLSKIGLIIENTGQLRSKL
                     NSGNDKTKK"
     gene            complement(30671..>31134)
                     /locus_tag="PLE23_00940"
     CDS             complement(30671..>31134)
                     /locus_tag="PLE23_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HMX81858.1"
                     /translation="FGIELTTPGLHLPDAGTKANILYTTVEAFAGSTLGQGSFEREEF
                     DSFSIPGVSGTGLVAFPIKGNSMEPVIQNGDIVVCREVNSINNIKENEIYAIKANGSL
                     WVKYIKKMTDSSGRVTHLKLISANHFEHDPFEEEVNEFTRIYKVIRKISEI"
BASE COUNT         9147 a         5938 c         6732 g         9317 t
ORIGIN      
        1 tcctgcaaat ctctttttct cgacaggtat tcctgtttgt attttggttt taaaaaagtg
       61 taaaaaattt gacgatgttt tgtttatcaa tgctgttgac cactttgaaa aaggaaaacg
      121 acaaaacagt ttacttcctg agaacattga caagatagtt aacacataca gaaaccgaac
      181 agaagaaact cgttacgctc gtagagtgac aatggacgaa attgttaaaa acgaattcaa
      241 cttaaatatt tcacgttatg taagcacatc attagacgaa gaaataattg acttgaaaga
      301 ggtaaacaaa aaactcgttg accttgacaa agacattaac aaagcaagag agacacataa
      361 taaattttta aaggaattgg gacttccacc aatataaaac tcattttaaa cccatgctat
      421 ttgaaagtat aacttgtctg ggttagttta ttttgcttta tgaaatgcgt caaggataga
      481 aggcggcacg gagtaagccg gatagcctga cccgccaggg ggacgccctt atgaaagtat
      541 tgattattca gtagagacgt tgcatgcaac atctctacaa caaccggata aaaacgaaca
      601 aatggcggat atttccccca aatcccgttc aatatctacc ttcattcggt tcctataaat
      661 ctgccgttac caaacacgcc caccgtttgg gttttgaatt tgaatggcaa acccgttttt
      721 acgaccatat catccgcaat gacgaatcat ttcaaaaaat atccaattac atcaacaaca
      781 atactgcgaa ttggcaggaa gataaattta ttaattgaaa gcaggccggt atcgaggctc
      841 cggtgacgga ctatattttt cataagagat tgcttgaagg gtgggcgtat tattgccggc
      901 aggaattttt ttttggattt ttgttggtag gaatattatt ctataatgct ataacgtaga
      961 gacacctgag taggtggagg aataatttgt aattcctgac cgatctttat cgggccgatc
     1021 agaaatatta gggttatgca agaaaaatga aaagaggcgt gactttaatt atccaaaagt
     1081 aagataatca gtaaaaagca gatgatcgaa aaaagtaccc acgactggat tcgaaccagc
     1141 acatccgtaa ggacactacc ccctcaaagt agcacgtcta ccaatttcgc cacgtgggta
     1201 tcgaattgga agtgcaaata taaaacattt tcacctaatt ataccaaaaa ataaaagctg
     1261 aatattttgt ccgtattcta atttatgtag atcggtggta ggcaaaacgg taccattgct
     1321 tagctaaatg ctgctaacag acaaccgaaa catatataga ttcgtatgaa tatgagatat
     1381 ttttaaaaaa taattaaata aatatttgta aattaaatat gttgtcgtat ttttgtagta
     1441 acatatcaaa catatttttt aaacatacat taatcccttg aacacatgga acaattttta
     1501 ctgttatccg gtcttatctc tgttattggt tttatgacat cttgcctttc aaaatcacag
     1561 acttctgagg tgaaggcata atattcagag agcagttttt tataattttc ttgctgcatc
     1621 acgcagacgg cgaaggaaag gggaggcaaa gatgaattcc tgtaggagtt cgttgttgct
     1681 accgagtact tggcttttgt tgccattcca ttcgacgagg ccattgtgga gaaatacgat
     1741 attgtcacct atctccatta cactgttcat gtcgtgcgta ttgatgatgg tggtaatgtt
     1801 gttttctact gttatagact ttatcagttc gtcgatgagt atagatgttt ttgggtcgag
     1861 cccggagttg ggttcatcgc agaacaggta tttgggctcc agaacgatgg ctcgggctat
     1921 gcctacgcgt ttttgcatac cgccgctcag ttcggaagga tacttgttat tggcgttttc
     1981 gagattgaca cgagccaggc aataattgac gcggtcgatt ttttcttttt tggtcatgtt
     2041 ggtgaacata tcgagcggga agcggatatt ttgttcggta gtcaaagaat cgaacaaggc
     2101 agagccttgg aagagcatac ctacttccat tcttattttt cgcaatgctt ttttatcaag
     2161 actgaacaag tcggtattgc cgtaccatat tttacctgaa gttggctggt tgaggcctac
     2221 gagtgtcttg aggaaaactg ttttgccggc gccacttcta ccaatgatga ggttggtttt
     2281 gcccggataa aactcagcgc tgatgccttt gagaaccaga ttgccgttga attccttaac
     2341 tatattttct gctcttatca tcctgtcaac aatactgcga ttacataatc tgacaataaa
     2401 atgagaatat tgctgtacac gacagcacgg gtgctagcct caccgagttc gatattgcca
     2461 cctttgacgt aataaccttg gtagcacgac accgaagtga gaatgaaagc aaaaactgtc
     2521 gctttgacat acatcataga tacattgcgt ggcagaaaga attcgtgaag tcctttgaca
     2581 tagtctgtag aggtaagcca tccggtaggt acactagaaa ggtagccgcc tactatacct
     2641 acgaatgctg ctatggctac cagcatcggg atgacgacta aggctgccaa gatctttggc
     2701 aatacaatat aaccggctgt gttgacgccc attatctcca ttgcatcgat gtgttccttt
     2761 tggcgcatgg cacctacttc ggctgcaatg tttgagccta cttttccggc aagtacaagc
     2821 cctgtgatgg taggggcaag ttctatgatg gtcatgtcgc gtactacata gccaatgtaa
     2881 tatttgggta tcagttgacc accgagttgg taggcaaact gtatggcagt aaccgcaccg
     2941 ataaacactg aaataagtat gacaatgacc agcgaaccga tgcctatatc gttcatcata
     3001 cgcagcattt cgttccagta catgtagaac ttctccggtt tgccaaggga gagttttagc
     3061 atttggacat aacggccaaa atggaaaaag aaatttctga acataccagc ttgcttatat
     3121 tcttcagcga ttgaagtttc actggcaaat ttagattatt tttggaagca tcttctatat
     3181 atttatactt ctattgactt gtgtagtata tttttttgga aaatgatcaa cagattctgt
     3241 atttttactc aacattcatt tcttaatagt tatatgaaaa aagtaatagc aataaccgga
     3301 gcaaccaagg gaataggcag ggcgatagct gaaaaatttg cacaaaacgg ctatcatgta
     3361 gctgtttgtg ccagaaataa agaagacctt aagcaaatgg ctatatactg gacagaacat
     3421 tatccggaat ccgaaatttt tacattcagg gcggatgtca acagtaaagt gcagctgcag
     3481 gcttttgcta atcatctact tgcaaggttt ccggtagtgg atgtagtggt caacaatgca
     3541 ggtgtgtatt ttccgggtga agtgctgaca gccgacgaag ggacactgga gaaaatgatg
     3601 gaagtcaacc tgtacagtgc ctataatttt acgcggatgt tgatgccgtc actcaaaaag
     3661 cagccggcag cgcatatatt taatatgtgc agtatagcaa gcattcttgc ataccctaac
     3721 gggggtgctt atagtatatc gaagtttgcc ttgcttggtt tctcaaaatg cctgcgcgaa
     3781 gaactcaagg attcaaccat aaaagtaact gcaatattgc cgggggctac ctggagcgat
     3841 tcatgggcag gagccgattt gcccgtagag cgcctggtgc aggcagcgga tattgccgat
     3901 acggtgtatg ctgcaagcca gctgggtaaa tcagcagtcg tagaagaaat aattatccgg
     3961 cctcagcaag gcgatcttta gttttaaaat aaaatatact ttcttgtatg cttgttgtca
     4021 tacaatgtaa gtaattggat attctacacg ttgagtccgc cacagatgct gatgacctgc
     4081 ccgttgatgt aagaagacat gtctgaacct aagaaaacac ataagtttgc tacttcttca
     4141 gcagtgccgt atcgcttcat aggtatgcct gatagaaaag cttcttttgt tttttcatcg
     4201 agctcgccgg tcatttctgt atcgatgaat ccgggagcaa tcacgttggt acggatgttt
     4261 cttgagccga cttctttggc aatggacttt gaaaagccga tgataccagc tttggatgct
     4321 gcataattgg cctggccggc attgccaaac acaccaacta cagacgacat gttgatgata
     4381 gagccgccag tgcgcagcat cgccttcatg cagtgtttgg taaggttaaa taccgatttg
     4441 aggtttgtgt gtattacctc atcccattgt tgttccgaca tccggagcat caacgtatca
     4501 cgtgtaatgc ctgcattgtt tattaaaata ttcaccgggc ctaggtcggt ctgcactgca
     4561 gctaccaaat ctccggcttg tttgaaatcg gcagcatcac tcttatagca ttcggctttg
     4621 acacccaagg cacgaagttc atcacgcaat gcttcggctt tagttgccga cgacacataa
     4681 gtaaatgcaa cattggcacc ttgttcggca aattttttaa cgatagcagc acctattccg
     4741 cggcttcctc ctgtgatgag tgcattctta ccttccagta gtttcatgtt gtggtgtttt
     4801 ttcaaggcta aagtacgatt tttcttcagt ttttgataaa aattgaagga agtaaaatat
     4861 tttttaaaaa aattaaaaaa acattttgtt tataaataca agttgtttta tatttgcatt
     4921 gataaaaaca ttttacaatt aaaaagcatt aaattatgaa gaagtcaagc atcatcaggt
     4981 tatttatcat tggattattg gcagttttgt ttgtattgcc tttgaggtca cagtttcagt
     5041 taccttccaa tgaaggtgtt ggatataggg tcaagcagtt aaggcagtcg tatcaactaa
     5101 caactgctga gcttgcaaaa tatacaggcc ttagcaaaca gtatctcgaa ggtatagaat
     5161 gtggaaaata tgcagtcggt gataccgagt cacgaatcat ttcagagtat tttggtcagg
     5221 agttgtaatc gtgtttcagg gtttacttgt tattagatta ccggtctgtt tttgaagaaa
     5281 agattgtggg acatggcagg taagggtata aaagaatgta ctgatgtcat tttgagtctt
     5341 tcaagttgtg gttaattatt gactgagttt tgttttagat aaacagagtt tttgtaatgt
     5401 gggatggggc agaagtaatg ttaaaatttg gcattacatt tgcttggtat tggttactta
     5461 ttagaagaaa catgtatatg ttaagataaa aattaacaaa cttctgagat aaccaccaaa
     5521 cccatcccca caggttacaa cctttgggga ttttttgtgt gtactgctcc ctttacatcc
     5581 atcttcaggt tctgttatgt gataggacaa atatttaccg ataggtaaaa atatgggcag
     5641 attttttata caggcaggta gggttcgcaa gttgagcaca caggttagga gcataattta
     5701 tgaacttagg ttaagaatac agattaataa ctcaggttag caacccaggt tagtaactca
     5761 ggttagtaac tcgggttaaa ttttgagagt gtgatttggt ggcgactttt agcggctgaa
     5821 tgttggttgt aatgattttc aggcattgct tgtagtgtag tgtagtgtag tgtagcggtg
     5881 ttagtgctac tcaccgactg aggccaggtt attttttgtt cggatggtag agtattgata
     5941 tagccttaaa gattattgac tatgagtatc cggtgtattg tttgatagtc agatacctca
     6001 aaggtattta tatagcagag aatgaatggt cagaacggta atttggtctt gtatcttcaa
     6061 tcaaatacac gtggcaagtt gagtcaagaa taaaagttca gagcataaaa ttcaaagctt
     6121 gatacaaagc cgcagccata gaaagctttg gtatatgagc actgaatgga tgattataat
     6181 tagaactgga aaccaagcat gaattgtagc gcaaaattac ggtcaaaatc atttcgcttg
     6241 ataaatttgc cagcgtcaag tttttgtaat gagatatcgt atgtttcgcg acttacatct
     6301 ttaaggccat aatacatatt agcgccaaga taaagtgaac tgttgaaata gtaagcaaga
     6361 cctagcaagg ctccaagatc aaagctttca tagtatccgc cggatttttc tgtaaagttt
     6421 tcgtaggcat tcaagatgct ccgagtagct atggcttcgg tgttcaggtt aaagtttacc
     6481 ggattgccgg ctatggagcc gattttgtca ctcccgtagt tatagtcgag ctcttgtacg
     6541 agtgtattgt cttttaaagt ggtagtaccg ttgaatacat gctttatttc gcctgttcct
     6601 gttgatgaaa tgagaaagcc agggctgatg ccggctgata tttctatttt tttgaagagt
     6661 ttggcataca agctcacggg caattggata tagctgttga aggtgttcag attatatcgt
     6721 ttgtagccgg tggtaacaat tggcttagag tcaatagtgg taaacttttg atatgcattt
     6781 tgcgattcga atagtcttct acctccgttg atactgtatt ggagctccac tcgcgcgcca
     6841 aatatattgt taaatggaaa gccggcattg acaccaacca gaaagcctgt attgttactg
     6901 aatttttcga gtgtatttcc gtcgttgtct gtttcggcag gaccaaggaa tctggaagca
     6961 tttagtccgg ctttaaagcc aagtttgaat ggcaattgcg cttgggcgaa attgacagaa
     7021 atcagcaaga tacagacaac aaacagattt tttttcattt tttttacttt aaaggcacaa
     7081 agataaacct atgaatccat aacagaacgt cccaaatgat aaaattgttt gtaattttgc
     7141 aacacaatgg gtaatttgac tgatataaga aagatttctg aacaggaact gaaggctgcc
     7201 ttagcagcta atgatgaacc gtcttttagg gtaaagcaga ttcaggaatg gatgtggaaa
     7261 aagtcggtcg tatcattcga tgaaatgacc aatttaccta aatctttaat tcaaaagcta
     7321 aaagagcagt ttgcatttta cccattggta cttgacacag cacagtttag tatggatggt
     7381 actataaagt caagattcag attgagcgat ggttacctta tagaatctgt gctgatacca
     7441 gtgcccgaag atgatcgttt tacggtttgt gtatcctgtc aggtggggtg ttctcttgcc
     7501 tgtacttttt gtgctacggg ccgtatgggt cgaatacgaa atctttcacc tgccgaaata
     7561 tacgatcagg tggtaatggt caatactcaa tgcctgcacc aatacggaaa gcctttgact
     7621 aacattgtat atatgggtat gggcgaacct atgttagctt accgcaatgt gctagatagt
     7681 attcgtctta ttacatcgcc tgatgggctt aatatgtcgc ccagacgtat cacagtaagc
     7741 actgcaggta tatcgaagat gatctgtaaa ctggccgatg atgaggtgaa gtttaatctg
     7801 gctgtctcgc ttcatgcacc cgatgatgtg aagcgaaata ccatcatgcc aataaatgaa
     7861 acgaatgacc tgaaatcgct aattgaggct ttaaggtatt tccagcagaa gaccaacaat
     7921 cgtttgacat ttgaatacat tgcattgcgt gactttaatc ttgaggacga ggatgcagag
     7981 aatctcataa aaatatataa gaaagtgccc gcgcgtatta acattatcga atacaacccg
     8041 atagagaatg cgccctttgg tcatgcacct gatgataagg ttgatgagtt tgctcaaaaa
     8101 ttgcgaaatg ccggtgttat ggtaacagta cgcaaatcga gaggcaagga tattgatgca
     8161 gcctgcggtc agttggctaa taagtaatca actgtataat tggcttttat accgacgttt
     8221 tattcatcat cattactatc ataattcaat ttgatattaa actttgtgag ttttacaaat
     8281 tcattgagtc tgtcaacaat ctcatcgtgc gtaagcgttt gtatcttttg cagactgaat
     8341 tcttctacac aaagtgatgc catagccgaa ccgtaaatca tagcacgttt catatagttg
     8401 tactgaatat tgtccgattg tgcgaggtat ccgatgaatc caccggcaaa tgtatcacct
     8461 gcaccggttg ggtcaacgac atcctgtaga ggtagggcag gtgcaaagaa aattgaattg
     8521 cggtagaaca gtagagcacc atgctcacct tttttgatga ccaaataact cggccccatt
     8581 ttgaggatag catttgcagc tttcaccaaa gaatgctctc cggatagctg tcgtgcttca
     8641 tcgtcattga tagtaagtaa atcaacctta ctcagcactt tcttcaaaga aggcattgct
     8701 acatccatcc aatagttcat ggtgtccatt acgatgagct taggtcttgc tgttagttgt
     8761 tctaaaaccc tcagttgtat atccggcgtt aggttgccaa gcatcacata ccttgatttt
     8821 ttatacgatt ctggtagtat ggggtcaaag tcggcaagaa cattgaggtc ggtggccaat
     8881 gtgtctcgcg agttcatatc gttgttgtat ttgccttccc agaaaaatga tttctggcct
     8941 tttcttatct gtaacccttc ggtatctact cctcttgctc tcaagtggtg gatttcagtt
     9001 tccggccagt cgtcacctat gaccgaaaca agttttacat ttcgataata gaaagttgca
     9061 gcccatgaaa tgtatgttgc tgctccacct attacttttt caacacttcc aaatggcgtt
     9121 tttatagagt caaatgccac tgttcctaca actaataagc tcataaaaaa ttttttttaa
     9181 aaaatgattc tgcaaatatt gcaaattatt tttaattgtc atacatttgc atcgcttttg
     9241 aagcaattgc atctttagct cagtcggtta gagcggcgga ctgttaatcc gtaggtccta
     9301 ggttcaagtc ctagaagatg cgccaaaggc tttccagatt ctggagagcc tttttttatt
     9361 tttattccca tggtattatt gctaaagcta ttttccataa taccttatca aatattgtcc
     9421 ggacaaatgc cgtacttcgc tgatggtgac atagcttttt ccattcagat cccagcagat
     9481 tccttcaccc tgaggctcta ttgtgtaaga tgattcaact ggtatggtct tgatgaggct
     9541 atctgtcggt atggaatgaa tgttcttcca caaatatact ctggtataat ctttaatgag
     9601 tatgtcgcct ttggctgaaa tgtcggcacc tgttacttcg gctataggca aatctgtaat
     9661 gtgttgtgca ataatattgc ccaaaggtgt tgctggcaat gtaagcgaat atactgaact
     9721 gtactttgac ttggtgataa tatatgatgc gccggttgtt ttgtcggtca taaatgcttc
     9781 tgcattgtag ctgactttgt ctggataact gaaggtaaat ttacttatat cgcttacaat
     9841 gaattccttt gggaattttc ctaccggaat cttgggttct tcaaaacggt agatattgta
     9901 ttttctgtgg atgtactggt tatcgccgat atcgctcagg taaatataat ttttcccttt
     9961 ttctggtccg ggtcctgtac agatgtcttc ccagtcacgg ttttttattc ctttcagtaa
    10021 aatttttact ttcaatgcac ccagactgtc tatcagataa atttcttcag gattgccact
    10081 gtcattatgt acgtaataat gcccgggtga gtatgcactg gcagctatac cggaggcttc
    10141 ttcaagttga tccgactgaa tccggaacaa ttcgcctttt gagaaattag tatcgtgatc
    10201 taatttaatc gggttgtagt gaaattctgc agaatctcca atattagccg tacatccgtt
    10261 cattgcgcga atacttattg cgataacaag tattttcaat atgttgaaaa tgcgttgctt
    10321 taaaagtttt aataccaagt tgttatgcat ctgttgttgc attttcgtcc gttttttacc
    10381 ctatgtcatc aaagtttaga ttgttacaag cttcatcttg tcaagcccat tcaacgaatt
    10441 agactcttta cttcaaatca ttattcattc atttccattt ccaaaactca ttacccagcg
    10501 ttcactttaa tttttttatg gattttcttg tacgaattct ttctgttaaa attcataatg
    10561 cgtggtctgg attaaatttg tgcccaaatt aggaataatt atgatgaaac tctgcttcat
    10621 ttttattggt tttatacatt cagttacttc tattggccag cccagtgcca atgtgaaagc
    10681 atttgcaccg gaagagcttc gaatgcagat gatgacaaaa tcagatgttc taatgcttga
    10741 caagttattg tgtgacaatc ttttatacat acattcgaat gcagttgaag aagataaggc
    10801 actacatctg gccaatattg catcaggcaa aatccggtac cggacgatgg atatagagca
    10861 aactgcctat tttacaaaag gaaaatttac ctttacaaat ggcatcctca gagtaaacgg
    10921 agtatatgat tcgaaagatt ttgatatacg gcttaaatat accgccgttt accaaaaaca
    10981 aaagtgtagg tggaaactgc tcagatggca aagcacaaaa attaacaact gaccttcttt
    11041 aacgacacgt ccgcttgtta gtacccattt atgggtaaaa tatttctgtg tattaagttt
    11101 taacttattc ttattccggc gctatgccgg cataagaata agttaagaaa tgcagtttcg
    11161 atcaaagaat attttaccgg atgatattta tttcgcctct gtataatagt tttacaccat
    11221 cgaggaattc tacttcaatg atatatacta ccacacatgg atttaatctt tgtccgttta
    11281 cttttccgtc ccagcccgta gtgtagtcac ctgcaggtat atctttggct tcatacagca
    11341 atgctcccca acggtcgaag attttcatgt aattgatttt gacaacaccg gtgccggtac
    11401 ctaccctgaa tacatcatta atgccgtcag catcaggtga aaatgcattc ggaatatata
    11461 cattgcggtt gttatctaca tcaataaata gtacatctga accgatacat cctttgtcgt
    11521 cggtgatagt aagttttact tcaattggtt ctgtcggata aatatacgga ttaagtgtat
    11581 tggggttgtt gaatgcttca gcaggtgtcc atgttatggt agccatgttt gatggtagcc
    11641 cctgagggtt taactgtatg ctgtcaccga gttctacctc tatatccggt ccaaggttaa
    11701 cggatacagg tgctggctca cttatactga ctacctcatc aatatagcag ccttttttat
    11761 caaacaccgt tatcaggtga tctccggcaa aaatggatac cagctgacca ataggtaatg
    11821 caggtccgtt gtcaactgag aatgtatagt ccggtccact gccacctgct gctgaagtca
    11881 ctgtcacaaa ggtctggaaa ccattacatt caggttcttc aggggtagga atgactgccg
    11941 tgagtggcga tggtggctgg atggtataag atacagaatc tgtacaaccc ttggcatcgg
    12001 taacagttac agagtagata cctgcaccta gattgcctgc gtattcggta gtggatacat
    12061 tcgggctcca ggtgtatgtg gtaacaccag agttgcctcc ggtgaaaaat acctgaattt
    12121 caccatcaga atcactggag caagtgatac cgtgtgtaat attcggatct aactgtgctt
    12181 gaagtacagg tggctcattg atgatatatg gtatagaaat actgctgcat cccttagcat
    12241 ccgtaatagt aagctggtag gtgccgggtg cgaggttacc aagtatattt gtagtagaag
    12301 tgccggttga ccagttataa gtatagggcg ctactcctcc gttaagattt accgtgatgg
    12361 aaccgtctgt caggccattg cacgtagtgt tttttatctc tgccggtatg atttctattt
    12421 cctgagcggc aggtatcaat actgagtcaa tgataccaca attgttgtca gtgactgtta
    12481 cggattgcca tccggagcat agctgtaggg caacactagc attcacaccg gtaaatattt
    12541 cggctgattc ccaaacgaac ttaaatgtgc ccgggttgcc atcgctgtac agagcatctg
    12601 caaatgccgt tccggtacaa gtacctgtag cacacgttgg tgcggcaatg ttaagaaact
    12661 gaagtgttat ctttggcggg tcgttgagca caatagtttc tatgtatggc ccacagttct
    12721 tggcatctgt gacagataca taatatgtgc ctgcaccaag actggctata accgcctgtg
    12781 atgtagggac tcccggattg gtactccatg tgtatgaata tggtggggtg ccacctgaca
    12841 tttgtacggc tatcgaacca tttttatcgc caaagcattt agggcttacg agggttttgt
    12901 ttaaaagtga aatgggtgcc gggctccaca acgttgcgga gtcaatagcg gcacaaccat
    12961 tattgtcagt aatggtaaca aaatactttc cagatttcaa attagtaatc gtagcggtat
    13021 tggtagtggc ggatggaccg ctccatgaat atgatactat ggtagaaccg gttgggatat
    13081 tggttttaac agtcaactgg ccatttgagt cttcaccaca ttttacacta acagaatcaa
    13141 atcctgtgat agtaatacca gatacctgaa tgatagtaac atttagagtg ttcgaacatc
    13201 cctcaacgtc ggttactgtg acactgtaat tgccggcatc aagatctgtt cgtgtaggtc
    13261 ctgaaatatt gccatcattc caaagaaaat cgtaaggtgc tacacctccg gtgatgtcca
    13321 gagtggcgga tcctttacct gtaccgccgc atcctatgtc ctgcttgctt gcgaggctga
    13381 caattacttc tgccttattg agcacattgg taaattctgc agtacattgg ttagagtctg
    13441 taatggtaag cttgtaactg ccgttgccga tgtttgacag cgaagtagca ttgggcccgt
    13501 tgagcccggt actccaagaa taactgtatg gttgagtgcc tccattggtg agtattacat
    13561 caatgaaacc gtcgtttgca tcatggcagg taggctgctg tatgttgaat tgaggtgtaa
    13621 taacaccagg ttgctgtacc tgtatggtaa gggtttgagt ttctatgggt tgactgttgt
    13681 cggtaacagt aatggtataa atacctgatg ccagaccgtt tatgatgcct gcaccaccga
    13741 gtgccggaat attgccattg ccattgactc caggtgagcc tacttttacc cagttgtatg
    13801 tatatggtgc cttgccacca agcgttacga gttgtatgct gccatctgta ctgccattgc
    13861 agataggctg tacaactgtt gttgccacct gcagaccagt attttgaggt aaaatacaga
    13921 tttcaccttc gatgaagttc tgcggtgcat cagtatagtt aaaactgccg tctgtaacag
    13981 aagtgtttgg cacttcagta gggctgagct catctatata tatcttgctg caatctaatg
    14041 gattgccgat aagcttaaag caaagctcaa ataatttgga accgtcgggc aaggtataac
    14101 cattgtctgt atcacttgct acaaaaagat taattacttc accttttgta ttttcattaa
    14161 aaagtatagt cgaattattg aagtcactta caaatttgcc gcttacatat tcgagtacat
    14221 ttgtgtcata gaagaaataa agtgtttgtc ctactacttt ttcccagtta tcaactgtaa
    14281 tgtctataca tactgtatca tttttgtaac caaggccctg accaatagta tatgtaacag
    14341 ccggaaactg gacctcagac ttgaattcgc aggttttgcc gtctgagatt tcgatgacgt
    14401 aggtgccacc ttgtggaacg ttgtaagaaa cagatgagcc gtgttttgca ggaccagagg
    14461 taatattgcc tttgatagcc ggattggctt taaggtaaat attaatggta taattctgtg
    14521 caccaagata ttcaggaaga ccgcctttta caatgaatgt gccttgaaag ctacccccca
    14581 taccggttgt agatgtaaag ttggcgtagg tgattttatt taaataaact accggaaatg
    14641 cattatttat atttacatcg acacaatttc cggcgggtgt gccttcaaat tttttattgg
    14701 cccagtcatc aagtgtaata ggtgcgaacc atatttgtac aggtttgccg ccattgaaca
    14761 gcaattgcag actaccgttg ttcttaaaat acatatcacc cgaaggagtt ttgccggtgg
    14821 ctacccacag accacccgga gcgggcggaa tatttagaac tgaaggatct gaaaggatag
    14881 cacttaatgt ctggccaggt acggtaggcg cgtttttata aaatgcatac ccaataccgg
    14941 gtggtgtagc tggtacaggg tctccggcaa ggtcctggcc gccttgatgg tctatgaaaa
    15001 gcgaatcgcc aaaacaaaga tataccgggt cattggactg tacacctgca cccaggctga
    15061 ctgttgcagt gcctgcatta ttgcatttaa gctcttttaa agatatgggg gctgctttgc
    15121 catcgaaaga tatagaataa ggagtttgag caaatatcaa tacagcaaaa aattgcaata
    15181 cgacaattgg gattattctt tttatcattt tttattactt gttaaggctt tttttgccag
    15241 gtctgttgtg atagaatcta aaattggctt aacgtctttt actgattttt atttccattc
    15301 cgggaatatt aattacaata aaattactta gacataattt tattgtaatt ttccttttaa
    15361 gatgactttg tcaaccaaat cgctgccaaa agaatagggt aaatatgcca gtgaaggaat
    15421 attttttgta atgatgatac taccttcata tcctttagct atcatgccgt atgtatcttg
    15481 tacttccatg gcacaagcac cgttgatggt cgctgcattt attgcttctt caggtaacat
    15541 tttcatataa atacaagata atgaaataac gaaactcatc cttccggaag gactcgaacc
    15601 cgggttgtaa tctgttgcta aagctacagg cagcccggca tcaatcattt tacgtgcagg
    15661 tgcatattcg agtttcagaa aaaaggcagt agatggtaaa agagttggta tggtggtgct
    15721 gttgagtaat gcattgattt cgtcttctcc aacgcattca agatgatcga ctgaaatagc
    15781 tgagtgtttt acacctgtct gtatgccacc tgaaaagtcc agttcattgg cgtgtatttt
    15841 gggtttcaac ccatacttcc agccagcttc cagtaatctg tcggtatctt ctgtggtaaa
    15901 aaagcctctg tcacagaaaa catccatata gtcggcaagt ccttcgcctg ctacttccgg
    15961 caacatatcg ttgatgatgt gcgatacata ttccgcctgt ctgcccttgt actcaagtgg
    16021 tactgcgtgt gcagccaaaa aagtggattt gataagacag tcggtggatt gtttcagccg
    16081 gcgaatgacc ctgagcattt taagttcact gtcttttgac aaaccatatc cgctttttat
    16141 ttcgagagcc cccgtcccgt atccgatgac ttctttaagt cgtgcagaag cctggtcaaa
    16201 taattcatct tctgtagcgg ttctgagcct tgcagccgaa ttgagaatgc cacctcctcg
    16261 ttttgcaatg tcttcataag aaagtccgtg tattttatca acgaactcgg cctcacgact
    16321 accgtcatac actatatgtg tgtgcgaatc acaccatgcg ggcaacacgc tctgtcctgt
    16381 acagtcaatg ataggtacat ttaagcttgg cagagaagac atcgaaccaa aatcttctat
    16441 ctgtccgtct ttgatgagca gaaatgcatt attaatcgaa ggaagtacag aaagatcctt
    16501 gcctttaacc ggagttttta ggtcagttcg ggtctggaaa agtgaccgga tattagtaag
    16561 cagaacggta ttggaagcca tggcattaaa attcgccaca aagctaacca aactttatat
    16621 ttttttcaat atttaaagtg aagaaaggtc tttaacgaag ccatctaaac cgttttcttt
    16681 aagttcagcc ttaatttttt gagcagattc cttacttatg taaacaccga tgaccaatct
    16741 tatctggttt tgcttgttga gtattagaat gtcacggtct gccaacagtt tttgtatccg
    16801 gatagtttcg gccaaggctg tctcgatatt tgaaaataca ccaatctgta caccataagt
    16861 attcgctgca acgttcggca ctttgctttg attttgaaca ttgaccaccg gaaccgatgg
    16921 agttgtaccg gatttggtag tattggtttc ttggtttctt ttttcagctt tgttaatagt
    16981 gtctatattt gcttttttgc ttgtaacatt tgttgttgca gatgctacaa attttaacat
    17041 tgctgtagaa gataagaatt ccttctttct ggctatttca gtaccatttt catcaagaat
    17101 gataacagat ggcaattcct taacttcaaa ctccgacatc agcctgaacc cttcgcgtgt
    17161 gtcgatgtca actactgtgt tgatggtatt gtcgttcaga aaacggacaa ctgcaccatc
    17221 agtcagcgtg ttctccttca tttttataca aggtgcacac catttggctg agaatatcag
    17281 gacctgtgcc ttcgatagtt gtttcgattt ggcagttgct gtattgaaat ctgcttcgat
    17341 ggagagtgtt tcgttgaccg gttctgttgc cagagctgcc gttgctgtca atatacaaac
    17401 cgttagaatg attatccctt tcattttact tatttgttta atacatattt cttaaaacct
    17461 taaatatagt aacttaaata tataaataat tgttacatga aatttgttaa attttagatt
    17521 attttcttgt caggcttttc tgttatttat ttgacaagtt tgtttatctt cgattcccga
    17581 attatttttt gagagtataa tatgtttgtt gtaaatgaat ggagataaaa aaatcagggc
    17641 tgctataacc gggatacagg gctatgttcc ggaaaccgtg ctttcaaata tcgatctgga
    17701 aagaatggta gaaacgactg atgagtggat aacaactcgt acaggtatca aggagcgcag
    17761 gatactgaag gagccaggca aggcactatc cgatatgtgt acagaggcgg tcaatggctt
    17821 gttgaggaag accaatacag ctgcagacga tattgagttg ctgatatgcg ctactgttac
    17881 tccagatatg gtttttccgg atactgccaa cattattctg gacaaagtag gcgctaaaaa
    17941 tgcttatggt tatgatataa gagctgcttg tagtggtttt ctgtatgcac tttataccgc
    18001 ttcaaaactt gtagaaagtg gtgcatacaa gaaagtgatt gtagtaggtg gcgacatgat
    18061 gtcttctatc attgattata cagaccgtgc tacatgtgtc atattcggtg atggttgtgg
    18121 tgctgttcta ctggagccaa caacagacga aacaggcatt gtagatgctg tactcaaggc
    18181 agatggcgca ggacgtgttc atcttcacat gaaggctggc gggtcgctca aacctgcaag
    18241 tattgaaacg gtcatggcta aagagcatta tgtgtatcag gagggtcagg cagtctttaa
    18301 atttgctgta aaaggcatgg tttcggccat agaagaagta cttgccagaa ataatatgac
    18361 aacagatgac attaactggc tggtacctca ccaagctaac aaacggatta tttcctctgt
    18421 gggtgatttt ttaaatatcc cttcacaaaa agtgatggtg aatatagaaa aatatggaaa
    18481 taccacatca ggtactattc cattgtgcct gtgggaatgg gaaaaccaac taaaaaaggg
    18541 agataatatt atgttgacag cttttggtgg cggatttacc tggggtgcat tgttgttaaa
    18601 gtggtcgtat gatggttctg cacagaacag atagatatag catttgctac aaaaaattaa
    18661 aattcgtaca tttgcggcga aatttaaatt tttaacaaat aaataattga atggcaacca
    18721 caggagatat caaaaacgga ctttgttttg tgtataataa cgacatttat actgttgtag
    18781 agtttcaaca tgttaaacca ggaaaaggtg ctgcctttgt tcggacaaaa atgaaaagcc
    18841 ttaccaacgg tcgtgtcctt gaaaataatt ttccttcagg acacaaaata gaggtggtaa
    18901 gggtagaaag aaggaaaagc caataccttt tcaaagatga ggcaggatat aattttatgg
    18961 ataatgaatc ttttgagcag tttaccattc cggatggcat gatagaaaat gctcagttga
    19021 tgaaagaagg tatcgaagta gagatcttgt ttcatgctga aaaagaaact gctctcaccg
    19081 ttgacctccc gctacatgtt gtactgcagg ttacatacac agaacctggc ctcaagggtg
    19141 acactgcaac caatgcaatg aaacctgcaa aactcgaaac aggagcagaa attagagtac
    19201 ctttgttcgt taacgaaggt gatctgataa agatagatac acggaccaat tcttacatgg
    19261 aaagagctaa ataattaaaa tcatgactta taaagaaatt caggaactga tcaatctggt
    19321 agcaaagacc aatttgtctg aagtaaagct cataaacggg gattttaaac tgattatccg
    19381 ttcgaaggag ttttcatccg gcaaagcagt acaagtagta gcccctgcac ctgcttctca
    19441 accggccact caggtcgtgg caccacccgt ctctaccgag ccggcaatat cgacggtaac
    19501 tgagaaggca gtacagccaa cagagccaac aacaggcgga aattacatcg aaatcaaatc
    19561 tccgatggtc ggtactttct acagagcttc aggcccggat aaaccagctt ttgttcaggt
    19621 aggtgacaat gtgagcaaag ggcaacgcat ttgcataatt gaggctatga aactctttaa
    19681 tgaaatagaa agcgaagtgt caggtaagat tgtgaaggtt ttggttgatg attctactcc
    19741 ggttgaatat gaccagcctt tgttcctggt tgatcctgca gggtagcctg tgcttccaat
    19801 ttcacccaat taacaaacca gcaataatta actatgttta ataaaatcct tatcgccaac
    19861 agaggggaga tcgcccttcg gataatcagg acatgtaagg aaatgggcat caaaactgta
    19921 gctgtatatt ctactgccga cagagagagc ttgcatgtta gattcgcaga tgaagccgta
    19981 tgtatcggtc cgccaagttc aacagaatca tacctgaata ttccaaggat tatggctgca
    20041 gtagaaatta ctaatgctga tgcactccat ccgggttatg gatttttaag tgaaaatgca
    20101 aattttgcag aagtatgcca agagtacggc attaagttta tcggtcctac agcagcgcag
    20161 atccgaaaaa tgggcgataa ggtaactgcc aaagaaacta tgattaaagc gggtgtacca
    20221 gttgttccag gatcggacgg actgctaaaa gatactacca aaggaaaaaa actcgccaaa
    20281 gaaatcggat atccggtgat actcaaagct acagccggtg gcggaggccg tggtatgcgc
    20341 atagtgtgga aagaagaaga gttcgaagat gcctggaact cggccagaca ggaagccaaa
    20401 gctgcctttg ccaatgatgg tatctatatg gaaaaatata tcgaagaacc acgacacatt
    20461 gaattccaga tagcaggtga ccaatatggt aatgtttgcc acctttctga acgtgactgc
    20521 tctatacaac gtcgccatca aaaactggtt gaggaggcgc cctcaccttt tatgacagat
    20581 gaacttcgcc aaaaaatggg ggaagctgcc gttaaagccg gcaaatcaat caagtatgaa
    20641 ggtgtaggca ccgtcgaatt cctggtggat aaatacagga atttctactt catggaaatg
    20701 aacactcgta ttcaggtgga gcaccctgtg acggaggaag taatcgacta tgatttgatt
    20761 aaagaacaaa taaaaatagc tgccggtgtt cctatttccg gaaaagacta ttacccaatc
    20821 atgcacgcca tcgaatgcag aatcaatgcc gaagatccaa aaaataattt cagaccttcg
    20881 ccaggcctta tcaaaacact gcacatagcc aaaggtcatg gtgtaagggt tgatacccac
    20941 gtttatacgg gctataaagt accgtctaac tatgactcga tgatagccaa attgattgtc
    21001 aaggctcgta caagggaaga agctatcacg aaaatggaaa gagcacttga cgaatttata
    21061 gtggaaggta tcaaaacgac tataccattt cataaaaaac tgatgcgaga tccgaaattc
    21121 atagcaggtg attttaatac tgcgtttctg aatgattttg acttctctga tctggactaa
    21181 aatattgtcg ggtagataac taaataagat acatgtcaaa gttgtccagg ctgttgggtt
    21241 atgtaaaaaa atacaaatac ctggtagctt tgaatatttt ttcgaatatt cttctgtcac
    21301 tgtttacaat agcgaccata ccggctatca tcccattttt acagatactg ttcagacaaa
    21361 caccgcgtaa gaccataccc gtagaactaa agtctaacct cagcaatctt attgaattcg
    21421 ttgaatggaa attcagctac ctgataacca gtgtaggtga agaaaaggct gtgttttttg
    21481 cctgcggaat gctcatagtg ctgttctttt tctccaatct ttttcgctat gcggctttgt
    21541 atttcataag cccggtacgt tatggtgtta ttggtgatat acgcgaatcc gtcttctcaa
    21601 aaatcatgcg attgccgatg tcatatttta gcgaagaaag aaaaggtgac ctgatgtcga
    21661 gagttacggc agatgttcag gaaatagaag tgagcatact gagtgtgttg gaggcaatat
    21721 tccgggagcc actcatgatc attggcgcat tgacctatat gctttatact agccctacgc
    21781 ttacattgtt tgccttcgga atgatactcg ttattgggtt tgtagtgtgg gtcatttcaa
    21841 aaacactgcg caaacatagt agtgaggcac agcaaagact cggttctgta acaagtatca
    21901 ttgaagaatc tttaggcggt ctccgtgtta tcaagggctt caatgcagaa cagtaccagc
    21961 agagtaagtt tgaagcagaa aatcttttgt acaaaaagac gatggtgaaa atacttcgta
    22021 aaagagacat ggcctctcca ctcagcgaat ggcttggcgt caccattgtt gctttactta
    22081 ttgtctatgg ggcttctctt gtgtttaaag gtgacatgca ggcgtcggca ttcctggctt
    22141 tcttgtttgc cttcttcagg gtcatagatc ctgccaaaag cctttcaagt gcatggacca
    22201 atatccagaa gggttatgct gcgatggata gaatagatat gattatgcat gctgatgaag
    22261 caatcaaaga tgcaccagac gcattgccga ttcaaacatt caactcagct atagaattta
    22321 gaaatgttca tttcaaatat aaaaattcgg aaaccaccgt tctgaaagat atcaatctcg
    22381 tcattccggc agggaaaaca gttgcattgg taggagcttc cggatccggt aaaagcacat
    22441 tacttgacct tataccgcgt tttcatgatg ctacacaagg cacgattctt atagacggta
    22501 ctgacatcaa acaactaaag atatatgatc tgcgtgaact gttgggcatt gtaactcaag
    22561 atgccatcct gtttaatgat acggtgtaca ataatattgt atttggccag aaagatgtaa
    22621 cgcgtcaaca ggtggaagat gctgccagaa cagcgaatgc acataacttt atcgtggcta
    22681 tggaagacgg ataccatacc aatgtaggtg accgaggatc gaaattgtct ggcggacaga
    22741 gacaacgcat caccatcgcc cgcgcagtat tgaagaatcc tccggttttg ttgcttgacg
    22801 aagcgacatc tgctcttgac agcgagtcag aaaaattggt acaacaagca ttgaactacc
    22861 tgatgaagaa tagaaccagt ctcattattg cacaccgact ttctaccatc atgcaggctg
    22921 acgaaatact cgtcatgagt gaaggtaaca tcatagaacg aggtacccac gaccaactta
    22981 tggcacagaa tggcgaatac gcagctttgg tacaactgca gagcatctga acaaaatcga
    23041 tatatagtat taagtgtttc aagaaaagat gtatatttga aaaaaattta ttgatgatga
    23101 agaaatttac ttttcttata gcgtttgtct gtagtatggt gaatgtaaat gcacagataa
    23161 tcgttacaaa tgcagcgtgg ccaaaggcag gtgatacaac caaattagcc aatgattcag
    23221 agtccggtta tttattagct ccggcacagg cgactaatgg caattgggat ttttcaaagc
    23281 tcaaggaaga aagtatcgac cttacatcat ttgctcctgc aagtcaggga aacggttttg
    23341 cagattttcc gacagcagaa ctgtattcaa agtcaaattt tgctgatgta tattataacg
    23401 ttactaacag tgcttttgag attcttggat acaaaggaga tattctggga ttcggattcc
    23461 cgctggtagt caaaggaaag ccggctgctg tagaaagaag aaacccgatg aagtattttg
    23521 acataagcaa taccgaatac actttcggag ccaccttcag tgcagaatta cttcctgact
    23581 ccttgttctc aggcattccc tttacaccag actctgttag gtttggccaa aatatcaaaa
    23641 ggcttgatgt agtagattca tggggcaagc ttaaaattcc actcggaacg tatgatgtac
    23701 ttcgcgaaaa aagaacaagc atccagacta tcaaattgga agtaaagata cctgtgctcg
    23761 gctggacaga tgtttcgaat ttcttcggcg gtgccggtgg tactcctgat acttctgttg
    23821 cttaccatta tatagcaaat ggtagtacgg agcccattat ggtcttaaat tgtacagcta
    23881 caggcgatac catacaggat atagatttca aaaccaataa aacaaaagtt ggtatattca
    23941 gcccatcact atctattgga aaactcaata tcttcccaaa tccggctaat cattttatcg
    24001 agttaaatac cacaggcatc attgaaggca aagcacattt gatgattaca gacttgctgg
    24061 gcaagacaat gagggaagaa accgatattc tgaccaaaga accatataat tggtttatca
    24121 atcttgataa ttttaatgtc gggatgtatt ttgtatcgct taaggatgag aaaggtaata
    24181 tcaaagcttc cggtactttt agcattgtca gataatttat tttacaatta aaatattctt
    24241 aaagccatcg tatgctgaac ggtggctttt tttataaaaa tgttcggaca gtttcatttg
    24301 ccgaccccac tcgtcagact cgacggattc agttctcatc atgaagtgtt catgaaaaga
    24361 gatgatctta cacatcctgt gatatccggc aataagtatc gaaaattaaa gtacaatctg
    24421 cttgcattca aagaaggaaa ttatgaggct ttgatgacaa aaggcggata ttactccaac
    24481 catattgcag cggctgctgc cgccggaaaa tattttgaca taaaaactat cgggataata
    24541 cgtggagaga aacctctgaa tcccggagtg acgctcaatt tcgcccagag tgcaggaatg
    24601 cagctcagtt acatgtccag ggcagagttt agagaatttt cataccagcc tggtcatcct
    24661 gagttcgaga acatttattt tctaccggaa ggtggtacca atgagcttgc actcaagggt
    24721 tgtggtgaac ttgctgagga gcttgcctta cagatcgacc tgaacaatac gcttatctgc
    24781 ttggcatctg gtacaggtgg caccacagcc ggtcttgctg atgtgttgcc cgaaaatgtg
    24841 aaggtgttat ctttttcggc actcaaaggt aagtggttaa aagatgaaat aaacagccta
    24901 cttcacaaga atgtaagtca tgtggatgtg acagatgaat attgcttcgg tggttatgca
    24961 aaagcagatg acaggctttt tcattttatc aataactttt ataataagta tggcataccg
    25021 ctggatccgg tctatactgc aaagatgatg tatgggcttg aggaaatgtt aaaccaagaa
    25081 aggataacac ccggcaaaac cataatagcc atccatactg gcggattgca gggcgttttt
    25141 ggatttcagg gtagcaaagt acccattcat tggtacaatg atccattttg ccgggaggca
    25201 tcagcactta agagtagcat gttgtaatgc accggttgat tcattttatg tcaatatttt
    25261 caatattatt tatttttttt cttttcaatt ttaatttcta aaaattttag cttttctttg
    25321 ttttgaacaa tacttggaag atcattccgc attattgtgg gttcagaaaa ttaacaaatc
    25381 aaggaaaaga acatgagtac acctgtcatc atcatcgtta tgggcttttt cattttttgg
    25441 ggacattata tgaatggtgt ctttgagagg aggagcatac cggatgtgtt gggcctgatg
    25501 atactaggtt tgttgatagg cccgattctt aatcttgtag atacctccac atttggccag
    25561 tttggttcta ttttttcgaa tctggtacta ttattcatcc tgttcgagag tggtacagac
    25621 ctgaagtttg cggaggtaaa gaactcgctg agggcggcgg ctaccatcac taccattggg
    25681 tttcttttta catggatgac cctcacaccc ttgtgttatt ttatgcttga aatacctatt
    25741 ttaccttcac tatttattgg tgcagcgctc ggtggtacct cttctgccgt agtggttggc
    25801 ttagtgaaga aaatcaatgt caggccaaag accgctgcca cactcatcat ggagtcagcg
    25861 gaaacagatg tttttacact cgctattccg ctgagtattc taggtttgat gacctccgcc
    25921 aatgtacagg tgaataccgt gttgtcaagc tttatttcga gtatcatact ttcgcttatt
    25981 attggtatag gtggagcttt tctttggaca tttatactag gcaggtacaa agacctgaag
    26041 aataccaagt ttatgacacc tgctttcttg tttatcttat atggcattac agaatatttg
    26101 cactttagtg ggccattgac ggcattgtcc tttggtattg cattgggcaa tcttgattat
    26161 tttgaaccaa aaatgctcga gcgtattatc cccaatcaag acattgcatt gaagaaggac
    26221 gaaaaagatt tctttagtga aatggttttt ctgctacgca ccttcttctt tgtatatatt
    26281 ggtctgagta ttaaaatcga cgatcttttc tggcttacct ggggagctgg ttttacaggc
    26341 atattgtttc tggtgcgcat catagcggtc aaactggtaa tgcatcgaga tacaccggta
    26401 ctggataagg gcgttatcag ttttatgata ccaaaaggtt tgggtgcagc agtaatagct
    26461 acacttccgt tgcaggaagg ccacccttat ggttctgcta tacaggcatt gtgtttttct
    26521 attatattgt ttagtacaat ttatacctcg atcctatttt tcctcaccaa gcgaggaatt
    26581 accattcctt tatataaaat aatattcgga aaggacaaga atagtacagt gacaaacgat
    26641 acagcaatgt aagctgcaaa taattatagc ttcatgtcca tttcaaaagc tcaaaaccat
    26701 agttcactat gttatgatct tttttttatg gattttttgc atgaatcaat ttcgcttaaa
    26761 ttcttcatcc gttatctggg ttaactctta atggcagtca agtgaaatca ttgccacaca
    26821 aaaggcatca tcttcacggt tgatgtctat cagatgtttg gcataacgct gcagctccat
    26881 tgaaggattg ggttgtccca atatatcttt cactttagaa tcattcgtct ttatttcttt
    26941 tattttttcc ttatcaggaa attctgtctt gctcgcaaat acaccaaggt catgacctgt
    27001 gaaaaccgga cttgttcgta ttgcttcagg cagattatcg tagcctaccg gaatgatatt
    27061 taccggttgg tgatgcgaaa atatgcaatc gcccttaatc cgtgcgtacc atgctcgccc
    27121 aagtctgcct actaagtcag ctttttgggg atctattctg ttgttttcat ccagtataga
    27181 tgtagagatg tgcatcctgc tcacttggca aataatgaga tggcctgctc cgcctttttc
    27241 acccaaaggt atcacctctt tgactgaaca ttccatttgt acgggcgatt ctttgacgcg
    27301 gtatggtttt acagtctcag aaggtatagg tgttaaaccg gttttttcga attcgttaac
    27361 atccggagca tactccacgc tcgagatggc tacttgcctt gcaatggcat aattgacaac
    27421 attgatgact acttcttttg tggcaagcac attatgtagc gtgtcttttg ttttgttatc
    27481 tgctactttg cggtttgaag aaaataccag aatcggcgga tttgaactga acgcattaaa
    27541 aaagctataa ggagcaatgt tcggaattcc gttttcatcc attgtggatg caaaggctat
    27601 agggcgtggt gcaattgacc caacaatatg ttgatgcaga tcttttacag gcaaatcctt
    27661 tgggtcgata gtcagtaatt cgtccatatt tcaaaaattt tgcgaaattt acaattattt
    27721 acggatatac atgcaaagaa caatcattac agtctttatt ttgttaagta tttgggcggc
    27781 ctttaagctt gctcgcgatt attatttcaa accaaatgtg tctgcaggtg aacaaatacc
    27841 caaaacaatt gtacaactaa aggatggaaa agagcttgac ctggctgcct ggaaaggtaa
    27901 atatgtacta cttcatttct gggggagttg gtgtggtcct tgtagaagag aaagccccga
    27961 aattcggaca ctttatgaaa attttcatac aaaacaatat aataatgctg atggatttga
    28021 aatattgagc attggcattg aaaaggatga atcaagatgg aatagtgcca ttatagctga
    28081 ccgcctgact tgggactacc atacaacaga gcttaatggt ttcgaatcgc gaatggcaaa
    28141 actttatggt ataaggcaga tacctatact gctattggta gggcctgacc aacgtatgat
    28201 aggttcagca caaacacctg actcagtaaa agaaatattg accgagaaaa tcaaacaata
    28261 actgacaaat tgacacaata attcagatgg caagtaaatt gatacataaa ttgtttaaaa
    28321 caagaaagca agcaatgaca cagaagaata atgaaggtac aaacctgcac gcagatgatc
    28381 aggaaatggt ggaggtcaga gaagaagagc ataatattgc tgaagatgtt gaacaagtag
    28441 aacatccgga ccccaatgaa agtctgaatg cacagatagc cgagttaaag gacaaatacc
    28501 ttaggctcgt agccgaattt gacaatttca gaaaacgtac tgcaagagaa cggatagagc
    28561 ttgagcaagt ggcatcaaaa gaagtgatgt tggcactatt accggtgctg gacgatttcg
    28621 aaagagcaag aaagatagct gatgaagaag ccagtccgga gccattctca gaaggtatcc
    28681 gactggtata taataagttg actcaaactt taaaaagtaa aggcttgcaa gctatggaaa
    28741 cggatggagc caacttcaat gaagagatgc acgaagccat tactgagctt gacctaggag
    28801 gggccaatac aggcaaaatt atagatacca tcgaaagagg ctatacactt aatgaacaaa
    28861 ttattaaata tgcaaaggtt gtagtaggta aatagtatgc cccttggcaa atactcctgc
    28921 aacagacaca aatattatgg caaaaagaga ctattacgaa atactcggtg tagataaaaa
    28981 tgctgatgcc gatactataa aaaaagcata ccgcaaggtg gcaatgcagt atcaccccga
    29041 tcgaaatcct ggagataaag cgtctgaaga aaaatttaaa gaagcagcag aagcttacga
    29101 tgtgctcaat gaccctgaca aaaaggcaag gtatgacaga tatggtcatg ccggtgttga
    29161 cccaaatatg ggggcaggcg ggtttcgtgg tggcaatggc ggaatgacaa tggatgatat
    29221 tttccagaat tttggagacg tattcggaag tgataatccg tttgaaagct tttttggagg
    29281 cggatcatca agaggaggtc aacaaaaaca agggcagcgt ggaagcaatc tgaggattaa
    29341 agtaaaaatg accctggagg aaatagctaa gggtgtctct aaaaagatta aagtaagaaa
    29401 acaggtaaaa tgcacaagct gcaaaggctc cggtgcaaaa gacgctcaga gtaccagcac
    29461 atgctctact tgtcgggggt cgggctatgt caggcaaata aagaatacat ttctggggca
    29521 gatgcagacc actactacct gtccaacttg tagcggaagt ggacaggtca taacagcaca
    29581 ttgtaatacc tgcaaaggag atggccgaat gtgggaagaa gaaacaatag agattgacat
    29641 accggcaggc gtagaagaca acatgcagct gtctttgcgc aataaaggta atgccggtcc
    29701 gaaaggcggc ccgtcgggtg atctgctcat acacatcgaa gaggtaaaac atgaatattt
    29761 gactcgtgac ggtatgaatc tggcctatga actgaacctt aattttgccg atgctacctt
    29821 gggtacgagt gttgaagtac ctactattaa cggtaaggct cgagtaaaaa tagacccggg
    29881 aacacaggcc ggaaaagtac ttcgcctcaa aggtaaaggc ctgccttcgg tgcataatgc
    29941 atattctact ggcgaccaac tcatttatgt caatatatat acacctaaag atattacagc
    30001 tgaagagcgc actttgcttg aaaagatgag aaatatgcct aactttcagc cgaaaagtga
    30061 aaaatcagag aaaggcttct ttgaacgtat gaaggatatc ttcagttaaa tctctaaatt
    30121 acttggtatt gaaagttaaa gttatcttgt cggttggtct tgtcttgctt cttgccggat
    30181 gcggcccaga ttatattttt aaagattatc aaaccataga ttccaaaggt tgggattata
    30241 ataaacgcat tgattttcaa ttcgaaatca atgatacttc aaaggtgtat aatctcattc
    30301 ttgatgtgat gcatgcagat acttttgctt ctgaaaatgc atacgttaag gtttatacta
    30361 ctttccccga tggcaaaaag accgaacaac aactctctct ggaactagcc tccaaaactg
    30421 gaaactggtt cggcaaatgt tcgtcgggta gctgcaatct gctgataggt ctccaagaaa
    30481 atgtcgtttt cgctcagtcg ggtacttata aattcagcct cgaacaattc gggcgtagca
    30541 acccattgac cggattgtca aaaatagggc tcatcattga aaacaccgga caactaaggt
    30601 caaaactcaa ttcaggcaac gacaaaacca aaaaatgatt taaccattat agtcggcttg
    30661 acttgctggt tcagatttct gatatttttc tgattacttt gtagattctg gtaaactcat
    30721 tcacttcttc ttcaaatggg tcgtgttcaa aatgattggc tgatatgagc ttcaaatgtg
    30781 tgaccctgcc ggatgaatca gtcatttttt taatgtattt tacccaaaga gaaccattgg
    30841 cttttatggc gtagatttca ttttccttaa tattgtttat cgaatttact tcacgacaca
    30901 caacaatatc gccattctga atcactggct ccatgctgtt tccttttatt gggaatgcta
    30961 caaggcctgt acctgacaca cccggaatgg aaaagctatc gaattcttca cgctcgaagc
    31021 tgccctgccc aagtgtagaa cctgcgaatg cttcaacagt agtataaagt atgttggcct
    31081 tggtaccggc atcaggcaaa tgcagccctg gggtggtaag ctctatgcca aaag
//
LOCUS       DAOQQY010000007        31009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_424_length_31009_cov_7.137527, whole genome shotgun sequence.
ACCESSION   DAOQQY010000007 DAOQQY010000000
VERSION     DAOQQY010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 31009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31009
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_424_length_31009_cov_7.137527"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1671)
                     /locus_tag="PLE23_00945"
     CDS             complement(<1..1671)
                     /locus_tag="PLE23_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX81859.1"
                     /translation="MAKALNNKPKTKINVQLPSSRLAAVVLAFIGMLCYFNTLGHLFT
                     LDDFSVIKDNFVTKQGLAGIPTILTTDYRYGYWVSGGTLYRPLSQIMFAIEWALAPDS
                     PFIGHFVNVALFGLTIFVLFYLMKNYLTQSSVWLSAGICLLFATHPMHAEVVANIKSR
                     DEILGLLCSLGALYYYMLSYSHRGIKYSLLGFVLYLLAMFSKESAVTFLAVFPITAYF
                     FTNAGWQDILKKSWPLVLPVVIYLIVRQSIIGSNISLEQTSVLDNFLMAADNPFTRMT
                     SAVMMLGYYIKSFLFPLTLSHEYGFNQIAMAGITDWRFLLSLLVHVAMVWYILRYIKQ
                     KRLSVYGLLFYIITMSIVSNIFITIGTSYGDRLFYTPGLGLMIFVCAALYEYFLRNKQ
                     ETSFNHLAPGYKWPLIVVSVLFASKTFSRNAAWYDSYTLYKTDIEHAPNSAKMRYHFA
                     LETGKKAMEETDAAKQKEWRLMAIAQDTKALEIFPKYNDVFAHLGLMYYHLKDYSKAI
                     NNYTEAIKLGAKDAKMFSNMGTLYSETGDNKKAMEYYKKAVELDPRFVD"
     gene            1905..2408
                     /locus_tag="PLE23_00950"
     CDS             1905..2408
                     /locus_tag="PLE23_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMX81860.1"
                     /translation="MKWFFAFLFTAVISTGLSAQKFAYVDIDKILNSMNEYQAAQTEL
                     DNIASKWKQEIAQEYDKIKGMYNKYQAEQVLLSEDAKKQREEEITQKEKEVRDLQKQK
                     FGTDGALFQKRQELVKPIQEKVYNAIEKYAGEKGYDFIFDKGTAGIVFSGAKFDLTDD
                     IVKALQR"
     gene            2451..2960
                     /locus_tag="PLE23_00955"
     CDS             2451..2960
                     /locus_tag="PLE23_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMX81861.1"
                     /translation="MKRITGLLVFSLILMGTQLVQAQKFGFVNSAALLTELPEVKAAD
                     SELNVLQNQLKKKGEEMVTAFQQKYAELATKEKNGEYSPKQLEMESQKLKDDEAKITQ
                     FEQESTQAMSKKREELLQPILDKVNKAINDVAKESGYAYVFDASTNVLLYADPSADVT
                     ALVKKKLGL"
     gene            2966..3790
                     /gene="murI"
                     /locus_tag="PLE23_00960"
     CDS             2966..3790
                     /gene="murI"
                     /locus_tag="PLE23_00960"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMX81862.1"
                     /translation="MIFNNQPIGIFDSGIGGLTVASAVMRLLPEENIVYFGDTGHMPY
                     GDKSIELIRKYAVRIARFLIEDQHCKAIVIACNTASAAAYEYLRDELRGQIPVINVID
                     PMIERVVDDSSIHNVGIIATKTTVASGVYQEKFTRRKPELKYAAVATPLLAPMIEEGF
                     YDNSISHAVLENYLLLPQLREIDALILACTHYPLIKHQIASFYSDDVQILDSAEVVAL
                     KLKRILQKEKIMALEKPEKNDKFFVSDYTTSFEKTATIFFPDEIKLIKSDIWESQV"
     gene            complement(3801..4346)
                     /locus_tag="PLE23_00965"
     CDS             complement(3801..4346)
                     /locus_tag="PLE23_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX81863.1"
                     /translation="MKKLLDKLITRIVFVFTMFFNFACQSQRPDTGFLVKNRDFDNKL
                     KSLLSFNVPIIGVEEVSAHLSHYTLLDAREKSEYDVSHIQGAIHTGYDKFDLGILKNI
                     PKDAAIVVYCSVGYRSEKICQKLIKLGYKNVKNLYGSIFEWTNRGLTLYDYNEKPTSK
                     IHTYNKDWSKWVDNQKMTKVW"
     gene            complement(4411..5988)
                     /locus_tag="PLE23_00970"
     CDS             complement(4411..5988)
                     /locus_tag="PLE23_00970"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMX81864.1"
                     /translation="MKTLLLTLLTVFTLAFANAQAKWELGGQVGVMGYQGDLNPNKYM
                     DFNTLKLDYGLFLKRNFSKTIGLKLNYLGGQVAGDDTKWNAERKTRALKAKTSVNELS
                     LLLDWDILGKSRYREDGTFRKRFSPYLFAGVGAAFVGLPEVNYGHPNTPGGDPDYPNR
                     PNLAGIAKDQAATKPSVDFTVPFGAGVRIDLSRQWVLGAEYGLRPVFDDYLDGIKEGG
                     NNTDDDWYSSLGLNLSYRFGQKDTDKDGIIDKKDACPLVPGLAQFNGCPDTDGDGIAD
                     ISDACPNVAGLAQFNGCPDKDGDGIADKDDACPDVAGIAQFNGCPDTDGDGIPDKDDA
                     CPTVKGIAQFKGCPDTDGDGIPDKDDACPTVKGIAQFNGCPDTDGDGIPDKDDKCPTI
                     AGTKANNGCPEMKQEVVQAINLAVKNIQFETNSDVLRAESVPVVTQLAEILAQYPNYN
                     VNISGHTDSDGNDKANLMLSEKRANKCVEFLTSKGIAASRLTAKGYGETKPIATNKTK
                     AGKQANRRVEFELVKTN"
     gene            complement(6092..7168)
                     /locus_tag="PLE23_00975"
     CDS             complement(6092..7168)
                     /locus_tag="PLE23_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HMX81865.1"
                     /translation="MQRYQNPSLILESANIKGGKVHWTCPSNIALIKYWGKHGLQLPK
                     NPSISFTLDNAVTTTSVSYVASNDNRISFDLIFNNSRRDDFKPKIQTFLERISVIFPF
                     LKQLHLTIESTNSFPHSTGIASSASSMGALALCLCTLEEKLFGTSMPKNEFYRKASYI
                     ARVGSGSASRSVYPVAALWGEFADIKDSSDEYAIPVQDLLHPVFQSFQDHILIVSAEE
                     KSVSSTIGHALMDGNIYADSRYQQAKSRLHFLMPALKSGDLETFGKILEDEALTLHAL
                     MMTSNPSYLLMRSGTLSIIEKLRTFRMSTNIPAYFTLDAGPNVHLLFPENYTEIMSDF
                     IQNELSQYCNGNIINDKVGLGPKV"
     gene            complement(7238..7792)
                     /locus_tag="PLE23_00980"
     CDS             complement(7238..7792)
                     /locus_tag="PLE23_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HMX81866.1"
                     /translation="MNIEINGKSVPVLISDGDTMMIFDLKEVHVKAPHIFNNVSDEFL
                     YKRYKRYAIAVFPYAVKAIKVFRAMENDTKYMTSKEKKKYIKNLDKELQKEFEKPLKN
                     LSKTQGKILIKMIEKELDTPMYSLVKDLRGGFSAFYWNMSGSFYGYHLKDGYIVGADP
                     ILDLVLDDFDIDYTIRQEENFSTK"
     gene            complement(7937..9232)
                     /locus_tag="PLE23_00985"
     CDS             complement(7937..9232)
                     /locus_tag="PLE23_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMX81867.1"
                     /translation="MKSILILLLFIGQSLCSYTQNVGALPQRMPVAIKGATIHCANGK
                     TIQSGVIAFEAGKITILEDMRSSKILNQDKYEWIDATGKHIYPGLIALNTQLGLSEIE
                     SIKATNDFSEIGSYNPEVRALVAYNTDSRVTPTVRSNGIFLTQTAPVGGVISGMSSVM
                     MLDGWNWEDAVYKADDAIFMYWPYLNSPSRGSNNDAAKQNPTEQFENKIRELKSFFDQ
                     AYAYSAKINPQPRNLKFESLIPVFKGERKIWVRAHDVIEIQSAVQFFEPYSIKPVIVG
                     AQEAWKITDFLKEKNIPVVLSETHRLPMYEDDNIAQPFKTPAILADNSILFSISVDGF
                     WQVRNLPFQVGQAIPYGLDKETALNCITINAAKILGIDRSTGSLEVGKDATLLISEGD
                     IFDMMSSKVSMGFIQGRKIDLSNKQKVLNSIYRKKYNLE"
     gene            complement(9249..12263)
                     /locus_tag="PLE23_00990"
     CDS             complement(9249..12263)
                     /locus_tag="PLE23_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMX81868.1"
                     /translation="MKSRHVAGVLLLLLFHISSNAQQTFPINGISDERENYYAFTNAR
                     IYKSYNTFIDSATLIIRKGKIVSVGKNLNVPNDAVVVDLGFKTIYPGFIDIYTSYGMP
                     ESKPSKGFEYGRNSNLLPDRKGAYAGNDALKCEFSAIDHFTTDVGKAKQYLEAGFCAV
                     NTHRMDGISRGSSALVSLADQRENLLIIKDKTAHHLSFSKGSSTQAYPGSLMGIIAVL
                     RQTYLDGEWYARSGYKEQYNLTLASWNDLQKLPQIIECNDKLNELRALKIAEEYNKSY
                     ILKGGGNEYQRIDELKGKATQIILPVNFPDAYDVEDPNIADRINLTDMRHWELAPSNP
                     KIVSDAGIEITLTTSGLDKISSFKSKILECLNRGMSKEAILKAITYNPASWLGALDKI
                     GALDAGKLANFFICDGDYFDKNSKILQTWSNGEPTVFSETTDAIRPGKYKLSINNDPI
                     RILVFEKDGLKYKSFIQVNDTSKINVNNTINRNIITLSFVPDQKTKKFIRLNGTAYPD
                     KLIGSGYDITGNAINWKADFISDIEKSTKPSVTDTTNNVLVSTIVYPWDGYGWKEKPV
                     QKDYLIKNATIWTCEKEEVLKGYDLLIKNGKIDKIGKNLSFKGAIEIDASGKHVSPGI
                     IDEHSHIAIQGGVNECTQPNTAEVRIGDVVNCDDVNIYRQLSGGVVAAQLLHGSCNPI
                     GGQSAIIKLRWGYAPEKMKIENADGFIKFALGENVKNSRSSFNTRYPDTRMGVEQIYR
                     DAFTRAQEYLAAKKNNTLLASQRKDIELETLVEILTSKRFITCHSYVQSEINMLMHVA
                     DDFGFKVNTFTHILEGYKVADKMKKHQAGASTFADWWAYKYEVIDAIPHNSGILHDMG
                     ITTAINSDDAEMARRLNQEAAKAIKYSEMSPTEALKMVTINPAKLLHLDARMGSIKEG
                     KDADIVIWSASPLSIYAIAEITFVDGIKFFDRNEMEQQKKLIDEERNALIQKMLAFKK
                     SGGKTELFTSAKKIQYHCDTIGEGKEESIENH"
     gene            complement(12360..14321)
                     /locus_tag="PLE23_00995"
     CDS             complement(12360..14321)
                     /locus_tag="PLE23_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81869.1"
                     /translation="MTGVPVIFYTKLFLLTLLLTSICSCKSRSGLPPANTISDDVFYQ
                     NIDSFADTRIIKYQCPAFDRLTPNQRLLAYYLVQAGLSGRDIIYDQNYQFNLVLTRVL
                     EKIYLNHKGNKDTKDWKNYERYLKQIWVSNGIHDAFTGVKIQPLFTKEYFTNLMSESH
                     ESLSPELVSIMFDPRKDALLCNNDPAADQIRTSAVNFYNHELTEADIEVYYRNNEHKN
                     KNGKLTLSTDGFVKEIFWKTGGMYAGAIRQIIYWLEKAAAIAENKQQEHSFRLLIEYF
                     KTADPGVLEKYEKARAAIDNNKIDFQIGFNDISHDPLKLKAGFVTLVYIKDDISARQI
                     KMLRDSIQFFKDFAALALPAQDLQTKGVDNSFELLSAGGSYAPAWRKNQLPNYFTNIN
                     KAIVYTESKSLAREFAPSPEIYSGLVKYTAEASKFFQWIRNIFLPDNQNISTKPEWEN
                     LNIIDYAKADLQALYMIFDPMIFKRRLLGDEQAARTMYDHYITKSLLVQLHDTEPGKN
                     ITNPELRAKQLVATMAFEKGQSKNIIVKEKINGKTYFRINNYKGLRQIFGQLLKDLNN
                     ISDDNGALRSILVEKYGTTADQQLMAEVNARINSIGFSFHPAFIQPVFEPIKNANGQI
                     VDVQILYPDNFNSQMLFFSEKYRLLPNSN"
     gene            complement(14325..14915)
                     /locus_tag="PLE23_01000"
     CDS             complement(14325..14915)
                     /locus_tag="PLE23_01000"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMX81870.1"
                     /translation="MRLIILLGLCSLMILSACKQDSKGNDSASSGGSNLPMQNENSMS
                     ASPGNKTAFAPGEFDPRLTQDDLSKMMKYFDDRTLGSFQFDPRGFLYQMKSEGNGKSP
                     QLDDQVVVNYEGRLMDDKEFDSTFKTGKPLTYQVGKMIPGFDAAVMSLKPGGKGLFVL
                     PAFLGYENKSVKDHTGNELIPPKSILVFTIELVSVK"
     gene            complement(14993..15736)
                     /gene="fabG"
                     /locus_tag="PLE23_01005"
     CDS             complement(14993..15736)
                     /gene="fabG"
                     /locus_tag="PLE23_01005"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HMX81871.1"
                     /translation="MKRLENKIAIVTGGASGIGKATSIKFLQEGAKVIIWDVAEQKAL
                     ETVKELAQDGSSIEFMKVDTTSFENVQQAAQTVADKYGRLDILVNNAGVTRDATLKNM
                     TEEQWDLVINVNLKGVFNCAKAVMPHMVANNYGRIINAASVVALYGNFGQTNYVATKA
                     GVIGMTKTWARELGRKNITVNAIAPGFIATEMMLTIPENVLQGFKDKTPLARLGNPSE
                     VANVYAFLASEEASFISGTVISVDGGLVI"
     gene            complement(15903..16586)
                     /locus_tag="PLE23_01010"
     CDS             complement(15903..16586)
                     /locus_tag="PLE23_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX81872.1"
                     /translation="MKPSILYVEDDPTLSFVTKDNLEMQGYSVTHCENGKIAMDVFDQ
                     SKFDLCILDVMMPEVDGFDVAAFMRKKNTEIPIIFLTAKSLKDDKLHGLRIGADDYIT
                     KPFSIEELQLKIEIFLKRSKVYKSEKTESIFNIGLYNFDFNNLSLVSPQKSRTLTQKE
                     ADVLRYFIINKNTVIKRSAILENIWGEDDYFLGRSLDVFISRLRKYLSEDTSITLENI
                     HGVGFRLKV"
     gene            complement(16588..17865)
                     /locus_tag="PLE23_01015"
     CDS             complement(16588..17865)
                     /locus_tag="PLE23_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMX81873.1"
                     /translation="MENKAINRLVILGVIAIIGILAMQVFWITSLLQFRKQELDQSIR
                     IALIQVAKDLARSRKAEMPANTPIQLRGGNTYQVNINDVIDAGLLEFFLRKELTNKGL
                     KLDFEYTVHDCNTNEVVYGNTVFLSKNSAQSNKLKKLPKYSKLLYYFSVRFHSTNKYI
                     LSSIQLPVIFTSVLIFILLVFSYTLIFILRQRRLSQMQKDFVNNMTHEFKTPISTIKI
                     SGEVFLNNDYIKQDTRLERYAKIIVEQSSRLNEQVEKVLQVAKLDKSSVDLDTRTINL
                     HNLILSVAESFELRIQNTGGSLELHLAAADPNIYADSLHLINMLNSLLDNAIKYSKEN
                     VRIDVLTGENDRVLYLQVKDNGIGISKEDQKKIFEKFYRVSTGNVHDVKGFGLGLYYV
                     HSICKAHGWPLTVESEKGKGTTFTISFKKKKTK"
     gene            17948..18844
                     /locus_tag="PLE23_01020"
     CDS             17948..18844
                     /locus_tag="PLE23_01020"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HMX81874.1"
                     /translation="MSKPTIILLSLFLSLCFTIPEGRTQNPFDFAYRLPKTVQDSLNK
                     NVNPFDIPNKREVKPKISLVGGTKIKKAVSLHFNKSQVPSQDLFWIYFGAALFVAVIT
                     SLSRSVFTKISRSVWNMKQMNIFYREVSNFYLAPMILLYIFWIFNLSIFTYFFCHYYS
                     VPLISASVTAQIFAIFGVLTGVFVLKHLAWSFVAWVFPIEKEMNQYQFLLINYHIIIG
                     IILMLINLILGYIPTQYVQFFLVIGTILVLSIFLVRLVAGTIIGSKYIFSSLFHFLLY
                     LCTIEIAPILVLWKLIIVLTGY"
     gene            18857..19654
                     /locus_tag="PLE23_01025"
     CDS             18857..19654
                     /locus_tag="PLE23_01025"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HMX81875.1"
                     /translation="MDSTLKTKEVLETYKPVKTILITQPPPANAKNPYSEIVEKYGIQ
                     IDWREFTQVEPIDIKEFRRQRIRPDEFGAVIFTSKNSIDHFFRICEETRIKMPLDTRY
                     FCITEAISNYLQKFIVYRKRKVFTGEKSIHDLKNFLIKFRETEKFLVPTSNLGARELL
                     SYLDELKINYQESLISNTVSSDLSDLKDITYDMLVFFSPLAVKSLYDNFPDFKQNATR
                     LAAYGNSTIQAILDQGLHLNLKAPEPETPSMHMAILKYLQQSNTENS"
     gene            19705..20340
                     /locus_tag="PLE23_01030"
     CDS             19705..20340
                     /locus_tag="PLE23_01030"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HMX81876.1"
                     /translation="MLDQDFINRLELAILKQKPGREAQILMANPGRSMAEPIHARSAS
                     VLLLLVSKNASPHIVLTKRTAKYPDDKHSGQISFPGGSLDPTDANLQMTAIRETYEEI
                     GVEPEKLQIIGYLTNLYIPVSNFLVHPFVGYLRQEAIYKIQPDEVDEIIEVPVSMLMD
                     DNNIKKKNIRIFNGITLKDVPYFDFYGNTVWGATAMILSEFRQLVSNIQGK"
     gene            20389..23769
                     /locus_tag="PLE23_01035"
     CDS             20389..23769
                     /locus_tag="PLE23_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMX81877.1"
                     /translation="METIATYQPKNKIRLVTAASLFDGHDAAINVMRRILQSSGAEII
                     HLGHNRSVQEIVNTAIQEDVQGIAITSYQGGHNEFFKYMYDLLKEKGAAQIKIFGGGG
                     GTILPTEIEELHKYGIARIYSPDDGRHMGLQGMINDLLEQCDFAVGQNLNGEIKEISS
                     KNPKAIARLISAVENFPEQNTTLVTDLQKENTPKHIPVLGITGTGGAGKSSLVDELVR
                     RFLIDFKDKTIAILSVDPSKRKTGGALLGDRIRMNSINDGRVYMRSLATRQSNISLSK
                     HVHSAIEVLKAANYDLIILETSGIGQSDTEITDYSDVALYVMTPEYGAASQLEKIDML
                     DFADIIAINKFDKKGAMDALRDVRKQYQRNHKLFDKKPEEMPVLGTIASQFNDPGMNA
                     LYKKIIQTLAEKTHTGLLSQTELEGGISEKIYIIPPARVRYLSEITDNNRHYSQWVLQ
                     QADIARTLFSIKNTIEAVKKQNLPNKDKILEDLEKTYHETDIKFDGTCRRIIQEWEAK
                     KASYAADEYIYKVRDKEFRQATFTNSLSHTKIPKVVLPRFKDWGEILRWVLEENVPGE
                     FPYTAGVFPFKRTEEDPTRMFAGEGGPERTNTRFHYLSVDMPANRLSTAFDSVTLYGE
                     NPDFRPDIYGKIGNSGVSVCCLDDAKKLYSGFDLCDPKTSVSMTINGPAATICSFFMN
                     AAIDQQCEKYIYEHKLEHLVEEKLLELYENKGLKRPKYNGDLPEGHNGLGLMLLGITG
                     DKVLPADVYNQLKAKALAAVRGTVQADILKEDQAQNTCIFSTEFSLKLMGDVQQYFIN
                     NNIKNFYSVSISGYHIAEAGANPITQLAFTLSNGFTLVEYYLSRGMDVDSFAPNFSFF
                     FSNGVDPEYSVIGRVARKIWAKAMKEKYKANDRSQKLKYHIQTSGRSLHAQEISFNDI
                     RTTLQALYAIYDNCNSLHTNAYDEAITTPTEESVRRAVAIQMIINHELGLAKNENPLQ
                     GAFIIEELTDLVEEAVYKIFDEITDRGGVLGAMETMYQRSRIQEESLYYESLKHSGEY
                     PIVGVNMYLSRDGSPTIIPKEVIRATEEEKKNQIASLEALHDAKKDISADVLHNLQKA
                     ALNNENIFSHLMEASKTCSLGQITDALYRVGGQYRRNM"
     gene            23844..25106
                     /gene="fahA"
                     /locus_tag="PLE23_01040"
     CDS             23844..25106
                     /gene="fahA"
                     /locus_tag="PLE23_01040"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HMX81878.1"
                     /translation="MIKANDPTRISWVQYDGSTDFPVQNIPFGIMIAGGKHKVCSRIG
                     DYIIDIAALYELGFLNGIDLGLQHLENEFLNDFISLGKEVTNAVRDRLADVLDDKNTL
                     GAKAKSEKFLRPALETVMSMPVRIGDYTDFYSSREHAFNVGCMFRDPKNALMPNWLHL
                     PVGYHGRASSIILSDCDVKRPKGQQLPPDSEVPVFGPCKQLDFELEVAFIVGKSNHLG
                     ETISTANAEEYIFGLCLFNDWSARDIQRWEYVPLGPFLSKNFASTLSPWIVTLEALEP
                     FKTVGPVQETKILPYLEYSGEKNYDIHLQVSIKPEGSDPVVVSRSNFKYMYWNMVQQV
                     AHHTVNGCNLRVGDLCASGTISGPTPDSYGSMLEIAWKGTKPVNMNDGTTRSFILDGD
                     EVALTGYAEKNGVRIGFGDCKAKILPAD"
     gene            25142..26620
                     /gene="dacB"
                     /locus_tag="PLE23_01045"
     CDS             25142..26620
                     /gene="dacB"
                     /locus_tag="PLE23_01045"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HMX81879.1"
                     /translation="MSSIIILTSLLFLSIDTVLGQNALQQTIYNIALDEDFRHGSLSI
                     CIIDLQNDSMVASYQPNTSLVPASTVKIFTTYAALAILGQDFRFKTELQYGGEFVDST
                     LNGSVFIKGWGDPTLGSARFPRVDNIKALNRKFIKALQKECIYKINGYIVSDGSYYEP
                     SPVPSTWQYDDIGNYYGAGSFGLNFHDNEYTLYFKQNPEMWSRPVIERTFPTMPEQKF
                     INNVSSASPRSGDNTYIFGDPYSNVRLVTGTIPSGKGHFAVRGSIPNPPLTCANRLVK
                     DFSTIDSSSVTKGAIDIEEYIKTIGAPLFRHTFYTHYSPRLSDIILQTNHRSVNLYAE
                     TLLRELGKTLHAQGTVDAGIKVLYQFWKDAGLNTHGLFFEDGSGLSARNGVSAWHFTQ
                     ALSIARKDSLNFQTYYNSLPVAGKEGTVRRLLNGYKGKAEVRVKSGTMRRVKSYCGYA
                     KAEDGKEYAFAFIANNYSCSSGKVRDQVENILRALIISPKKD"
     gene            26665..27333
                     /locus_tag="PLE23_01050"
     CDS             26665..27333
                     /locus_tag="PLE23_01050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMX81880.1"
                     /translation="MYCRLLLLVCIISFVACRNEQSNHKNAEYPNVADSTVSENLQES
                     KDRDIWQNPDYIIGTFGDLTDKTVADLGAGSGYFSFKLLEKAGKVIALDIDKHFIQYM
                     NQKAAELPPVLRNKFEARLVDTNDPKLKENEAQIVLLSNTYIYIQDRINYFKRLRNSL
                     SPGGKLFVVEFKKEKLPVGPPESIKLSAGQVSEELTQAGFSNIKADSSRLRYQYIVMA
                     ENLK"
     gene            27434..28363
                     /locus_tag="PLE23_01055"
     CDS             27434..28363
                     /locus_tag="PLE23_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81881.1"
                     /translation="MKYLLTLIIVMVLGTVSLIAQAKKGTYKMNSETSKSKSASKLYE
                     RGSTIKDVSLLTQLSNTNSKFYIAPYEHGYVYSEEKKKSNSQAADYSFYFVGVSGNRF
                     DKPKTFALNAPTGLKPVAVSFSNDRLTMFATCLKPGSKTSYTIYQANKQNNVWSGWQE
                     LEIAKPFESAGFAVVNTAGNKMYLCAKSKIYKTGYDIYTSEYNDGWQKAILVQGDINS
                     DGDDMFPTLFNDVLFYSSGAKGGEGGKDIIFVNLKDNNRECFNAGNKINTNAGEQMMI
                     LNEDNKSGLLIRNAPIATKAEVFRIVADDLFIK"
     gene            complement(28378..29646)
                     /locus_tag="PLE23_01060"
     CDS             complement(28378..29646)
                     /locus_tag="PLE23_01060"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMX81882.1"
                     /translation="MKVDVILGLQWGDEGKGKIVDFLAPQYDLVARFQGGPNAGHTLK
                     FDNKQFVLHTIPSGIFRPGLINLIGNGVVIDPITLEREATTLDDAGIDYVSRLLISKK
                     AHLILPSHRYLDAASELSKGKNKIGSTLKGIGPAYMDKTGRNGLRAGDIFSADFKDKY
                     EILKKKHIDLLKSFPAVDFDLETEEKKWFSSIEFLKRFRFVNGEYYINEQLNQGKKIL
                     AEGAQGSMLDIDFGTYPFVTSSNTLTSGVCIGLGIAPSSIGDVIGITKAYCTRVGSGP
                     FPTELDNEQGEWLRQKGGEFGATTGRARRCGWIDLVQLKYTIMLNGVTKLVMTKIDVL
                     QGLEQIEVCDAYNIEGKVTTEIPYEINDVAIAPITKTYSGWQSSTAGIKEYEQLPQQA
                     AAYIDTLEESLGIKFAMISTGPERESLIVK"
     gene            complement(29734..30078)
                     /locus_tag="PLE23_01065"
     CDS             complement(29734..30078)
                     /locus_tag="PLE23_01065"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMX81883.1"
                     /translation="MNTNYHFSERGNAKVLFVDQLFNELENKQIITSVSELIENGQIN
                     FIIDLSKVPYINSAGLNCLLSIQHKTQKVGGQFSLSGVSPQVIKLFEITKLKPFFKFT
                     NGLEEASMAVTA"
     gene            complement(30142..30630)
                     /locus_tag="PLE23_01070"
     CDS             complement(30142..30630)
                     /locus_tag="PLE23_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMX81884.1"
                     /translation="MSEQPFSEKILEEVKSIFSAHLEKQGLRKTPERYAILEEIYKRT
                     DHFDAEALYIHMKTKHYRVSRATVYNSLELLVSCDLVKKHQFGKNLAQYEKSYGYKQH
                     DHLICINCNKVLEFCDPRIQQIKDMMGELLKFRVIHHSLTLYGECTGDCEHKKTSENK
                     KK"
     gene            complement(30775..>31009)
                     /locus_tag="PLE23_01075"
     CDS             complement(30775..>31009)
                     /locus_tag="PLE23_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81885.1"
                     /translation="AELVITGIDSGPGVIERLTQNISGKLGLNIRSFYIDGNEGYFEG
                     RISIVVPSENYLVMAIKALKGLDGISSVYKAEA"
BASE COUNT         9298 a         5956 c         6190 g         9565 t
ORIGIN      
        1 atctacaaat ctcgggtcga gttctacagc ctttttgtaa tattccattg cctttttatt
       61 atctcctgtt tctgagtaaa gtgtacccat gttgctgaac atttttgcat ctttagcacc
      121 cagttttatg gcttcggtat aattattaat ggcttttgag tagtctttca ggtgataata
      181 cataagacca aggtgagcaa aaacgtcatt atacttaggg aatatctcga gcgctttggt
      241 atcttgggca attgccatta atcgccattc tttttgtttt gcagcatctg tttcttccat
      301 tgctttcttg cctgtctcga gagcaaagtg gtatctcatt ttggcactat tgggtgcgtg
      361 ttcgatatct gttttataaa gagtataact gtcgtaccaa gctgcatttc ttgaaaatgt
      421 tttacttgca aacagaaccg aaacaacgat aagtggccat ttgtaaccag gtgccagatg
      481 gttgaaagat gtctcttgtt tgtttcttaa aaaatactcg tacaatgcgg cacacacaaa
      541 tatcataagc ccaagaccag gagtataaaa cagtcggtcg ccgtagctgg taccaatggt
      601 gataaatatg ttgctcacta tactcatcgt tatgatataa aacagcaaac cataaacact
      661 aagccgcttt tgttttatat atctcaatat gtaccaaacc atagcaacat gcaccagcaa
      721 gctgagtaag aaccgccaat cggtgatacc tgccatagct atctgattaa atccgtattc
      781 atgactgagt gttagtggaa ataaaaagga tttgatataa taaccaagca tcatcacagc
      841 gctcgtcatt ctggtaaaag gattgtcggc tgccatcaaa aaattgtcta atacagaagt
      901 ttgttcgaga ctgatattac taccaataat actttggcga acgataagat agatgacaac
      961 cggaagaacg agtggccatg attttttcag aatatcctgc cagcctgcat tggtaaagaa
     1021 ataagcagta attggaaaaa cagcaagaaa tgttaccgca ctttcttttg agaacatagc
     1081 taatagatac agtacaaacc caagtaagga atacttgata ccacgatgcg aataactcaa
     1141 catgtaataa tatagtgcac ccaaactgca cagcagcccc aggatctcgt ctctactctt
     1201 gatattggca actacctcag catgcatggg atgcgtagcg aacagcagac aaatacccgc
     1261 actaagccat actgaggatt gggtcaggta atttttcatt aaataaaaca agacaaaaat
     1321 cgtgagcccg aataatgcca cattaacaaa atgcccgata aacggagaat cgggggccag
     1381 agcccattcg atggcaaaca tgatctgcga aagaggtctg taaagagtac caccgctaac
     1441 ccaataacca tagcggtaat cggtagtgag aatggtaggg ataccggcga gaccttgttt
     1501 agtgacaaaa ttatccttta tgacagaaaa atcatcaagg gtaaacaagt gcccaagtgt
     1561 gttaaaataa cacaacatac ctataaatgc cagcaccaca gccgctaaac gggaagatgg
     1621 aagttgtaca tttattttag tttttggttt attgtttagt gcttttgcca tatttcatca
     1681 agatttgtca tttgcaaaac aaacacagag atcgttaaaa ttattattgt taataagcgt
     1741 taatacacat gattttttgg ttaatcaacc gttaaatcag aaaccggaga cgactttttt
     1801 acaaagaaaa gcattaattc gtatatagta ttgagagaat ggtctcaatc ttatggaaaa
     1861 atcttatttt tacaccgatt tttaaaatca aaaaatttaa agttatgaag tggttttttg
     1921 cattcctttt tacagcggtc atcagcaccg gattgtcagc acaaaaattt gcctatgttg
     1981 acatagataa aatactcaac agcatgaatg aatatcaagc tgcccaaact gaactggaca
     2041 atatagcttc taaatggaaa caagagattg ctcaagagta tgataaaata aaaggtatgt
     2101 acaacaagta ccaggcagag caagtattat tgagcgaaga cgccaaaaaa caaagagagg
     2161 aagagataac acagaaagag aaggaagtaa gggatctcca aaagcaaaaa tttggtacag
     2221 acggagcatt gtttcaaaaa agacaagaac tcgtaaaacc gatacaggag aaagtttaca
     2281 atgccatcga aaaatatgcc ggtgagaagg gatatgattt tatttttgat aaaggcacag
     2341 caggtattgt tttttcagga gcaaaatttg atctcacaga cgacattgtt aaagcattgc
     2401 agcgttaatc aaatcattat aatattttta ttaaacgaac aaacagtaga atgaagagga
     2461 taacaggttt attggttttt agcttgatat taatgggaac ccaattggta caggcacaaa
     2521 agtttggttt tgtcaattca gcggcactac ttactgaact tcctgaggta aaggcagctg
     2581 actctgagct gaacgtgttg caaaatcagc ttaaaaagaa gggtgaagaa atggttacag
     2641 catttcagca aaaatatgcc gaactggcta ctaaagaaaa gaatggtgag tattctccaa
     2701 aacaactgga gatggaatca caaaagctaa aagatgatga agccaaaata acacagttcg
     2761 agcaggaatc aacccaggca atgtcaaaaa agagagaaga gctccttcag cctattcttg
     2821 acaaggtgaa taaagcaata aatgatgtgg caaaggagag tggctatgct tatgtgtttg
     2881 atgcaagcac caatgtgtta ttatacgctg acccatctgc tgatgtgaca gcacttgtta
     2941 agaaaaaatt aggtctgtaa acaacttgat atttaataac caaccgatag gtatatttga
     3001 tagcggcatt ggcggcctta cagttgccag tgccgttatg cgtttattac ctgaagaaaa
     3061 tatagtatat tttggtgata cgggtcacat gccctatggc gataaatcaa tagaattaat
     3121 taggaaatat gcagtacgaa tagcacgctt tctgatagaa gatcagcatt gtaaagccat
     3181 cgtcattgca tgcaatactg catcagctgc agcctatgaa tatcttagag atgaactacg
     3241 cggacaaata cctgtgatca atgttattga cccgatgata gaaagagtgg ttgatgacag
     3301 tagtattcac aacgtaggta tcattgccac aaagactact gttgcttcgg gcgtttatca
     3361 ggaaaaattt acaagacgaa aacctgagct gaaatatgct gcagtggcca caccattgct
     3421 tgcccctatg attgaagaag gcttttatga taattcaatc agtcatgcag tacttgaaaa
     3481 ctatttactt ctaccgcaac tccgcgaaat agacgccctg atacttgctt gtacacatta
     3541 tccgctcatc aaacaccaga tagcctcatt ttattctgat gatgtgcaga tactcgactc
     3601 agccgaagtg gtagctctta agttaaagcg gatactgcaa aaggaaaaaa taatggctct
     3661 ggaaaaacct gaaaagaacg ataaattttt tgtctctgac tataccactt catttgaaaa
     3721 gactgccact atatttttcc cggatgaaat aaagctaata aaatcagaca tttgggagtc
     3781 acaagtctga gaatattatt tcaccagact ttggtcattt tttgattgtc cacccatttg
     3841 ctccaatctt tattgtaggt gtgtatcttt gatgtaggct tttcattata gtcatacagt
     3901 gtcaatcctc ggttggtcca ctcgaatata cttccgtaga ggttttttac atttttatac
     3961 ccaagcttga taagtttttg acaaattttt tcgcttcggt atcctactga gcaatagacg
     4021 acaatagcag catctttagg aatatttttt aaaattccta aatcaaattt gtcgtatcct
     4081 gtgtgtatag caccttggat gtgcgaaaca tcatattctg atttttcacg tgcatcgagt
     4141 aatgtgtagt gagacagatg cgcagatact tcttctacac ctattatagg cacgttgaat
     4201 gaaagaagtg atttgagctt attgtcaaaa tcacggttct taaccaaaaa acctgtgtca
     4261 ggtctttgtg actgacaggc aaagttgaaa aacatggtga atacaaaaac aatacgagtg
     4321 atcaatttat ccaatagttt tttcattata acgcaaaaag cctctccaaa gttgaagagg
     4381 ctttttgtta ttattaatag tgaaagagga ttagtttgtt tttactaatt caaattctac
     4441 acgtctgttt gcttgctttc cggcttttgt tttgttggtg gcaataggtt tagtttcgcc
     4501 ataaccctta gcggtaagcc ttgatgccgc tattcctttt gaagtaagga attcgacaca
     4561 tttgttagct cttttttcag ataacattag gtttgcctta tcatttccat cagaatcagt
     4621 atgaccggag atgttaacat tatagttagg gtattgagcc agtatttctg ccagctgagt
     4681 aactacaggt acagattcag ctcttaacac atctgagttg gtttcgaact ggatattctt
     4741 aacggccagg ttgatagctt gtactacttc ttgcttcatc tcaggacagc cgttgttggc
     4801 ttttgtacct gcgatagtag ggcacttatc gtctttgtca ggaataccat caccgtctgt
     4861 atccgggcaa ccattgaact gtgcaatacc tttaactgta gggcaagcat cgtccttgtc
     4921 aggaataccg tcaccgtctg tatccgggca tcccttgaac tgtgcaatac ctttaaccgt
     4981 aggacaagca tcgtccttat caggaatacc gtcaccatct gtatccgggc aaccgttgaa
     5041 ttgtgcaata cctgctacat ccgggcaagc atcatctttg tcggcaatac catcaccatc
     5101 tttgtcagga caaccattga attgtgccag acctgctacg ttagggcaag catcagagat
     5161 atcagctatg ccatctccgt ctgtatccgg gcaaccattg aactgtgcca gacctggtac
     5221 taatgggcac gcatccttct tgtcgatgat accgtctttg tctgtatctt tttgaccaaa
     5281 acgataagag aggttgagtc caagtgaact ataccagtca tcatctgtgt tattgcctcc
     5341 ttctttgatg ccatcaaggt aatcatcgaa aactggtctc agaccatatt ctgcaccaag
     5401 tacccactga cgtgagagat caattctgac acctgcacca aatggaacgg taaaatctac
     5461 agatggcttt gtggcggctt gatctttggc tattcctgcc agattaggtc tgttcgggta
     5521 atcagggtcg ccaccgggag tgttcggatg tccataatta acctctggca atccgacaaa
     5581 tgcagcacct actcctgcaa acaggtatgg actgaatctt ttgcggaatg ttccatcctc
     5641 gcggtatctg ctcttaccta aaatatccca gtcaagtaat agagacaact catttacgga
     5701 agtttttgct ttaagtgctc ttgtttttct ttcagcattc catttagtgt catcacctgc
     5761 tacctgacca ccaaggtaat ttaattttag acctattgtt ttgctgaagt ttctcttcaa
     5821 aaataggcca taatccagct tcaaagtatt gaaatccatg tatttatttg ggtttaggtc
     5881 tccctgataa cccattacgc ctacttgtcc gccaagttcc cattttgcct gagcattggc
     5941 aaaggctaag gtaaacacag ttaataaagt caacaataat gttttcataa ttgataaatt
     6001 aagtaataga ataagaataa ttgtttaatt gcacaaagat aacggttaat taatacaaaa
     6061 gatgcaaatt tattatgtaa aatttatgct atcatacttt cggacccaat ccaaccttgt
     6121 catttattat atttccattg caatattgag acaattcatt ttggataaag tcactcatta
     6181 tctcagtata attttccgga aataataaat ggacatttgg tccggcatct aaggtgaagt
     6241 atgcagggat attggtggac atcctgaaag ttcttaattt ttcaataata gagagggttc
     6301 ctgatcgcat caacaagtat gaagggttgg atgtcatcat cagggcgtga agtgtaagtg
     6361 cttcatcttc aagtatttta ccgaatgttt cgaggtcgcc tgattttaaa gccggcatca
     6421 gaaaatgtaa tcttgattta gcctgttggt atctgctatc ggcatatatg ttaccgtcca
     6481 tcaaagcatg ccctatagta ctcgatacgg acttctcttc tgcacttact atcagaatgt
     6541 gatcttggaa tgactggaat acgggatgga gcaaatcctg taccgggatg gcatactcgt
     6601 cggatgaatc tttgatatcg gcaaattcgc cccacaaagc cgcaaccgga tataccgaac
     6661 gggaagcgct gcccgaccct acgcgggcaa tgtaagaagc ctttctgtaa aattcgtttt
     6721 tgggcataga ggtaccgaaa agtttctctt cgagtgtaca aagacacagt gcgagtgcac
     6781 ccattgacga tgcagaagat gcaatgccgg tactgtgtgg aaatgaattt gtactttcta
     6841 tagtaagatg taattgtttc aggaaaggaa aaataacgga tattctttcc agaaaagttt
     6901 ggattttggg tttaaaatca tctctccggc tattgttaaa aatcaagtca aaagatatgc
     6961 ggttgtcatt actagccaca taggatacac ttgtagtagt gacggcattg tcaagtgtaa
     7021 aacttatgct cggatttttg ggtaattgga ggccgtgttt gccccaatat ttgataagtg
     7081 caatatttga cggacaggtc caatgtactt ttccaccttt aatattagcc gattcgagaa
     7141 tcagacttgg gttttgatac ctttgcatga tcgtgcattg aagtgcaaag ctaataaaaa
     7201 aataaacact gcctgctgta cctatcttgg tcgaacatta ttttgtacta aaattctctt
     7261 cttgtcggat ggtatagtca atatcaaaat catccaggac cagatcaagt ataggatcgg
     7321 cacctactat ataaccatct ttaaggtgat acccgtaaaa cgaaccggac atattccaat
     7381 aaaaggcaga aaaaccacct cgcagatcct taaccagaga atacattggt gtgtcaagtt
     7441 ctttttctat cattttaatt aaaatcttgc cctgtgtttt tgacaggttt ttcaaaggtt
     7501 tttcgaattc tttctgcaac tctttgtcaa ggttcttaat gtacttcttt ttttctttgg
     7561 aagtcatata ttttgtatca ttttccatag cgcggaaaac tttaattgct ttgactgcat
     7621 aaggaaacac tgcaatggca tatcgtttgt accgtttata cagaaattcg tctgatacat
     7681 tattgaatat atgaggtgct tttacatgta cttctttaag atcgaatatc atcatagtgt
     7741 caccgtctga tataagaacc ggaacgcttt taccgttaat ctcaatgttc atcaatttgt
     7801 cttgagacca taatggaaaa gtccaaacaa caaaagcaaa taacagaagt cgagtcatgt
     7861 agttaatttt aatattacaa acgaaaatct ttattgataa attcgtttaa caaagtcatt
     7921 aatgctttgt taacatttat tcaaggttgt atttttttcg ataaatacta ttgagtacct
     7981 tttgtttgtt gctgaggtcg attttgcggc cctgaataaa acccatcgaa actttgctgc
     8041 tcatcatgtc aaaaatatca ccctctgaga taagtaaggt agcatctttc ccaacttcga
     8101 gagatccggt acttctgtcg atgcccagaa tctttgcggc attgatggtt atgcagttga
     8161 gagcagtctc tttgtcaagt ccgtaaggta tcgcctgacc aacctggaag ggcagatttc
     8221 ttacttgcca gaatccatct actgatattg aaaacagaat agaattgtcg gcaagtattg
     8281 caggtgtttt aaaaggttgt gcgatattat catcttcata cataggcagt ctgtgtgttt
     8341 cacttaatac taccggtata tttttttctt ttagaaaatc tgtaattttc caggcttcct
     8401 gagcgcctac gatcacaggt ttgatgctat atggttcgaa aaactgtaca gccgactgta
     8461 tttcaatgac atcatgtgca cgaacccaga tttttcgctc acctttgaat accggaatca
     8521 atgattcgaa tttcaggttt cgaggttgtg ggttgatttt tgccgaatag gcataagctt
     8581 ggtcaaagaa ggactttaat tcacgtatct tattttcgaa ctgttctgtt ggattttgct
     8641 tggcggcatc gttgttgctt cctcttgaag gactgttaag atatggccag tacataaaga
     8701 ttgcatcatc tgctttatag acagcatctt cccagttcca gccgtcaagc atcatcacag
     8761 aagacatgcc agagataaca ccacctaccg gagctgtttg tgttagaaat atgccgttgc
     8821 tcctgactgt aggtgtaact cttgaatcag tattgtatgc caccagtgct ctcacttctg
     8881 gattgtaaga gcctatttca gaaaaatcat tggtagcctt tatagactct atttcactta
     8941 gccctaattg tgtattcagg gcaatgagtc cggggtaaat atgcttaccg gtagcatcta
     9001 tccattcata tttatcttga ttgaggattt tgcttgagcg catatcctca agtatggtta
     9061 tttttccggc ttcgaacgcg atgacgcccg attgtattgt cttaccattg gcacaatgaa
     9121 tggtagcacc tttgatggct acaggcatac gttggggcaa agcacctaca ttttgtgtat
     9181 aggagcacaa tgattgtccg atgaaaagca gcaatattag tattgatttc acttgatatt
     9241 atatttcttc aatggttttc gattgattct tcttttcctt cgcctatagt atcacaatgg
     9301 tactgtatct ttttagctga tgtaaacaat tcggttttgc cgcccgattt tttaaaagcc
     9361 aacatttttt gtatcaaggc atttctttct tcatctatca gttttttctg ttgttccatt
     9421 tcattccggt caaagaattt aatgccatca acaaaagtaa tttcggcaat agcatatata
     9481 ctcaatggtg aagcagacca gataacgatg tcggcatctt ttccttcctt gatacttccc
     9541 attctagcat cgaggtgcag aagttttgcc gggttgatgg ttaccatttt cagggcttca
     9601 gttggtgaca tttcactgta ttttatggct ttggctgctt cctgattgag acgcctggcc
     9661 atttcggcat catccgaatt tattgctgta gtaataccca tgtcatgcag aatgccactg
     9721 ttgtgtggaa tggcatctat cacttcatat ttataagccc accaatcggc gaaggtggat
     9781 gctcctgcct gatgtttttt cattttatct gctactttgt aaccttcgag tatgtgagta
     9841 aaagtattga ctttaaatcc gaagtcatcg gctacatgca tgagcatatt tatttcagac
     9901 tgaacatagg aatggcaggt gatgaagcgt ttacttgtaa gaatctcgac aagtgtttcg
     9961 agctcaatat cttttctttg gctggccaaa agtgtgttat tctttttggc tgcaaggtat
    10021 tcctgtgcac gggtgaaggc atcacggtaa atctgttcga cacccattct agtatcagga
    10081 taacgtgtat taaaagaaga tcgcgaattt ttaacatttt ctcctaatgc aaattttata
    10141 aacccgtcag cattttctat tttcattttt tctggtgcat atccccacct gagcttgatg
    10201 atggcggatt gtccaccgat aggattgcac gaaccatgaa gtaattgtgc tgccactaca
    10261 ccacctgaaa gctgcctgta aatattgaca tcgtcacagt tgacaacatc acctatacgc
    10321 acttcagcag tattgggctg ggtacattcg ttgacaccac cctgtatggc aatgtgtgaa
    10381 tgctcatcta tgatacccgg acttacatgt ttgccacttg catcgatttc aatagcgcct
    10441 ttgaagctca gatttttgcc aattttatca atttttccgt tttttatcaa taaatcataa
    10501 cctttgagta cctcttcttt ttcgcaggtc cagatggttg catttttgat tagataatct
    10561 ttttgcactg gtttttcctt ccatccatat ccatcccatg gatagacaat ggtagaaacc
    10621 agcacgttgt tggttgtatc ggttaccgat ggtttagttg atttttctat atctgaaatg
    10681 aaatcagcct tccagttaat ggcgtttcct gtaatgtcat agcctgagcc gatgagtttg
    10741 tcagggtaag ctgttccgtt gagccttatg aattttttgg ttttctgatc aggtacgaag
    10801 ctcaaggtga taatattacg gttgatggta ttgttcacat ttattttgct tgtgtcattt
    10861 acctgtataa atgatttgta tttcaagcca tctttttcaa aaaccagaat ccgtatgggg
    10921 tcgttattta tacttagctt gtatttgccg ggtcttatgg catctgtagt ttcagaaaag
    10981 acggtaggtt cgccattaga ccaggtctgc agtattttgg agtttttatc aaaatagtcc
    11041 ccatcacaga tgaaaaaatt ggctaatttg ccggcatcca gggctccgat tttgtcaagt
    11101 gcaccaagcc agctggcagg attgtaggta atggctttta gtatggcttc tttgctcatt
    11161 cccctattca ggcattccag aattttggat ttgaatgatg aaattttatc caaacctgat
    11221 gttgtcaatg tgatttcgat acctgcatcc gatactattt tcggattgga cggtgccaac
    11281 tcccaatgcc gcatatctgt gaggtttatt ctgtcggcaa tgttcgggtc ttccacatca
    11341 taggcatcag ggaaattaac aggcagaatg atttgtgttg ctttgccttt tagttcatca
    11401 atacgttgat actcgttgcc gccacctttg agaatgtatg atttgttgta ttcttcagct
    11461 atttttaggg ctcgaagttc attcagttta tcattacatt ctattatctg aggtagtttc
    11521 tgcagatcat tccatgaggc cagtgtaaga ttgtattgtt ctttgtagcc tgacctggcg
    11581 taccattctc catcaaggta tgtttgtcgt agtacagcaa tgattcccat tagtgagccc
    11641 ggataagctt gagtgcttga gcctttgctg aacgacagat ggtgtgctgt tttatctttg
    11701 atgatcagca gattttctct ttggtctgca agagaaacca gggcagatga accacgcgat
    11761 atgccatcca tacggtgtgt attgacagca cagaagcctg cttcgaggta ttgcttagct
    11821 ttaccgacat ctgtggtgaa atgatcgatt gctgagaatt cacactttag tgcatcattg
    11881 ccggcatagg cgccctttct gtcaggcaac agattagaat ttcttccata ttcaaaaccc
    11941 tttgagggct ttgactctgg cataccatag gaagtataaa tatcgataaa accgggatat
    12001 attgttttaa aaccaagatc gaccaccaca gcatcattag gaacattcag atttttacca
    12061 actgacacga ttttcccttt acgaataatg agtgtagccg aatctatgaa ggtattataa
    12121 gatttgtata tccgggcatt agtaaaggca taataatttt ctctttcgtc cgaaatgccg
    12181 tttattggaa aagtctgttg agcatttgat gaaatgtgaa acagcaataa caaaagtaca
    12241 ccagcaacat gtcgtgattt cattccggta atttttataa agcatcaaat gtataaaatt
    12301 ctttggaaaa tccaaccatg caaacaggaa gccgatttgg ttttttggtg tcaattgaac
    12361 taattgctat tggggagcaa acgatatttt tctgaaaaga acaacatttg tgaattgaaa
    12421 ttatcggggt acaggatttg gacgtcaacg atttgaccat tggcattttt gattggctcg
    12481 aaaacaggct gtatgaaagc cggatggaaa gaaaaaccta tagagttgat tctggcgttt
    12541 acttctgcca tcaactgttg gtctgctgtg gtcccgtatt tttctaccaa tattgagcgg
    12601 agcgctccat tatcatcact tatattgttc agatctttta acaactgtcc aaatatttgt
    12661 ctcagacctt tataattatt tatcctgaag tatgtctttc cgtttatttt ttcttttaca
    12721 atgatgtttt tactttgtcc tttttcaaaa gccatggttg cgaccaactg ttttgctctc
    12781 aactcggggt ttgtaatgtt tttgccgggt tctgtgtcat gaagctgcac caacagactt
    12841 ttggtgatgt aatggtcgta catggtgcgg gcagcctgtt catcacccaa caatcgtcgt
    12901 ttgaaaatca ttggatcaaa tatcatatac aacgcctgaa gatcggcttt ggcataatcg
    12961 atgatgttga gattttccca ctctggtttg gttgaaatat tttgattgtc aggcaaaaaa
    13021 atattcctga tccactgaaa aaatttcgag gcttcggcag tatatttcac taatcctgag
    13081 tatatttccg ggcttggagc aaattctctg gccagcgatt tgctttcggt atatacaatg
    13141 gctttattga tgtttgtaaa atagttgggt agctgatttt ttcgccaagc cggggcatag
    13201 cttcctccgg cacttaacag ttcaaatgaa ttatccacgc cctttgtctg caggtcttga
    13261 gccggaagtg ctaaagcagc aaaatcttta aagaattgaa tactgtcacg gagcattttt
    13321 atctgtcgtg cagatatgtc atctttgata taaactagag tcacaaaccc tgccttcagt
    13381 ttaagcggat catgggaaat atcattgaat ccgatttgga aatcaatttt attattgtct
    13441 atagcggcac gagctttttc atatttttct aacacgcccg gatcagcagt tttgaaatac
    13501 tcgatcaaaa ggcggaagga atgctcttgt tgtttatttt cggcaattgc tgcagctttt
    13561 tcaagccaat aaattatttg ccgaatggca ccagcataca taccaccagt tttccagaat
    13621 atttctttga cgaacccatc cgtactgagt gtcagtttac catttttatt tttgtgttca
    13681 ttatttctgt aatatacttc gatatcagcc tcagtaagct cgtggttata gaaattgaca
    13741 gcagaagtgc gtatctgatc ggctgcaggg tcgttgttgc acagtagagc atcttttctt
    13801 gggtcaaaca tgatgcttac caattctggt gaaaggcttt catggctttc ggacattaga
    13861 ttggtaaaat attcttttgt aaaaagtggc tgaattttca cacccgtgaa tgcatcatga
    13921 atgccatttg aaacccaaat ttgtttgaga tagcgttcgt agtttttcca gtcttttgta
    13981 tctttattcc ctttgtggtt taggtaaatt ttttcaagaa ctcgtgtaag cacaaggttg
    14041 aattgataat tttggtcgta aattatatcg cgaccactaa gcccggcttg taccaaataa
    14101 tatgccagga gtcgttgatt gggagtgagt ctgtcgaatg caggacattg gtatttgatt
    14161 atcctggtat cagcgaatga atcaatattt tggtagaaga catcatctga tatagtatta
    14221 gcaggaggaa gtccgctacg acttttacac gaacaaattg atgtcaacaa cagagtcaac
    14281 aaaaagagtt tggtgtaaaa aatgaccgga actcctgtca tatcttattt aacagaaacc
    14341 agctcgatgg taaagaccag aatagatttt ggcggaatca attcatttcc tgtatgatct
    14401 tttacggact tattttcgta acctaagaac gcaggaagca caaagaggcc cttgccaccg
    14461 ggtttgagtg acataacggc tgcatcaaag ccgggaatca ttttacctac ttgataagta
    14521 agtggtttgc cggttttgaa tgtagagtcg aattctttat catccataag ccttccttca
    14581 taattgacta ctacttggtc atcaagctga ggcgacttac catttccttc tgatttcatt
    14641 tgataaagaa aacctcttgg gtcaaactga aatgaaccca gtgtacggtc atcaaaatat
    14701 ttcatcatct tagacaggtc atcctgagta agccgagggt caaattcgcc cggggcaaat
    14761 gcggttttat tgcccggaga agctgacatt gaattttcgt tttgcattgg aagattagag
    14821 ccaccggagc ttgcgctatc atttccttta ctatcctgct tgcatgcaga cagtatcatt
    14881 aatgaacata agcctaacag tataattaat ctcatatatc ccagtttttg aaattatttt
    14941 acaaagatag agctttatag aggagagttg caacccttac aggcaatatt catcatatta
    15001 ccaagccacc atcaacactg atgacagtac ccgatataaa agaagcttcc tctgatgcca
    15061 gaaaagcata tacgttagcc acttcggatg gattgccaag tcgggcaaga ggagttttgt
    15121 ccttgaagcc ctgtagtaca ttttcaggaa tggtaagcat catttcagta gctataaatc
    15181 cgggtgctat tgcattgacg gttatgtttt tgcggccaag ttctcttgcc caggttttgg
    15241 tcatgcctat tacaccggcc ttagtagcca cataattggt ctgaccgaag ttgccataca
    15301 gagctactac agacgcggca tttattattc ggccataatt attggctacc atgtgtggca
    15361 ttacagcttt tgcacagtta aaaacaccct tgaggttgac attgatgaca aggtcccatt
    15421 gttcttcggt catgtttttg agagtagcat cacgtgttac acctgcattg ttgaccaaga
    15481 tatccagacg accatatttg tcagcaacag tctgagctgc ctgttgtaca ttttcgaatg
    15541 atgtagtatc taccttcatg aactcaattg aactgccatc ctgggcaagt tcttttacag
    15601 tttccagagc tttttgttcg gctacatccc agatgatgac tttggcacct tcctgtagaa
    15661 acttaatact agtagctttt ccaatcccgg atgcaccacc tgttacgatt gctattttgt
    15721 tttcgagtct tttcatttta tgatttacgc attaagaggc gcaaatttaa aagcattgtc
    15781 atgttaaaac ccaaatcttt tgataataag taggttgaca atttcaaaat atatcattaa
    15841 aaaaaatata aatacctgta aaataacata ttatacagta acaatattgg gttgacggat
    15901 atttaaacct ttagcctaaa accaacaccg tgtatgtttt cgagcgtaat ggacgtgtct
    15961 tctgacagat atttacgcag tcgactgatg aacacatcaa ggcttctgcc cagaaaataa
    16021 tcgtcttcgc cccatatatt ttcaagaatc gcagaccgtt tgatgactgt atttttatta
    16081 ataatgaaat atctaagcac atctgcttcc ttttgggtga gtgttctgga tttttgaggt
    16141 gatacaagag agaggttatt aaaatcaaaa ttgtacaagc ctatgttaaa gatagattca
    16201 gttttttcgg atttatacac tttacttcgt ttgaggaata tttcgatttt gagctgaagc
    16261 tcttcaatac tgaatggctt ggtgatataa tcatctgccc caatgcgcaa accgtgtagt
    16321 ttgtcatctt tgagtgattt ggctgtcagg aaaatgatgg gtatttcagt atttttcttt
    16381 ctcatgaagg ctgccacatc aaatccgtcc acctctggca tcattacatc cagaatacac
    16441 aaatcgaatt ttgattgatc aaaaacatcc atagcgattt tcccgttttc gcaatgtgta
    16501 acagaatagc cttgcatctc aagattatct ttggtgacaa agcttaaagt agggtcatct
    16561 tctacgtata gaatgcttgg tttcatatta ctttgttttc ttttttttga atgaaatggt
    16621 aaatgtggtg ccttttcctt tttcactttc tacggtcaaa ggccagccat gcgctttaca
    16681 aatactgtgt acatagtata atccgaggcc aaaacctttc acatcatgta cattgccggt
    16741 agatactctg taaaattttt caaatatttt cttttggtct tcttttgaaa taccaatacc
    16801 gttgtctttt acttgtaaat ataggacacg gtcattttct ccggttagaa cgtcaattct
    16861 tacattttct tttgaatatt tgatagcatt atcaagcaga ctgttgagca tattgataag
    16921 atgaagcgag tcggcataga tgttagggtc ggctgcggct aaatgtagtt cgagtgagcc
    16981 tcctgtattt tgtatccgga gttcgaaact ttcagctacc gaaagtatga ggttgtgtag
    17041 attgatggta cgtgtgtcga gatcaacgct tgatttatca agttttgcta cttgaagtac
    17101 tttttctacc tgttcattca acctactgct ttgttctact attatcttgg cgtatctttc
    17161 cagccttgtg tcttgtttga tataatcatt attaagaaaa acttccccag atattttaat
    17221 tgttgagatt ggtgttttga attcatgcgt catgttattg acaaaatctt tctgcatttg
    17281 tgatagtctt ctttgacgca gtataaagat aagagtgtaa gaaaatacta aaagaatgaa
    17341 aataagaacc gaagtaaaaa taaccggcag ctgaatgctc gaaagtatgt atttattggt
    17401 agaatggaat ctgacactga agtaatagag tagtttgctg tatttgggca gtttttttaa
    17461 tttgttggat tgggcagaat tttttgaaag aaaaaccgtg ttaccataaa ctacttcgtt
    17521 ggtattacaa tcatgaaccg tatattcaaa atcaagtttc agtcctttgt tcgtgagttc
    17581 ttttcttaga aaaaactcta gaagtccggc atctatgaca tcatttatgt ttacctgata
    17641 ggtattgcca cctcgtaact gaataggagt gttagctggc atttctgctt tacgtgagcg
    17701 agccaggtcc ttggctactt gtatcaatgc aatacggatg ctttggtcaa gttcttgttt
    17761 tctgaactgc aacaagcttg tgatccaaaa aacctgcata gcaagaatac cgataatggc
    17821 tataacgcct aatatcacca gcctatttat tgctttattc tccatgtttt attacaacga
    17881 atagttaaag ataaatacat attactgtaa aacaaaaaat taactttgca cttcaatatt
    17941 gatgctgatg tctaaaccga caatcatctt attatctttg ttcctgagtt tgtgttttac
    18001 catccctgag ggtaggacac agaatccgtt tgattttgct tacagactgc ctaagaccgt
    18061 acaagacagt ctcaataaga atgtcaatcc ttttgatata cctaacaaaa gagaggtaaa
    18121 accaaaaatc agtcttgtag gtggtaccaa gattaaaaaa gcagtcagcc tgcatttcaa
    18181 caaatcacaa gtgccgtcac aggatctgtt ctggatatat tttggagctg cgttatttgt
    18241 agccgttatc acatcattgt cacgaagtgt attcacaaaa atcagcagat ctgtctggaa
    18301 tatgaaacag atgaatattt tttacagaga ggtcagcaat ttttatctgg caccgatgat
    18361 attgctatat atattctgga tatttaatct ttcaatattt acctattttt tctgtcatta
    18421 ttactcggtt ccattgattt cagcaagtgt tactgcacag attttcgcta tttttggagt
    18481 gcttacaggt gtatttgtat taaaacattt agcttggtcc tttgtagctt gggtattccc
    18541 gatcgaaaaa gaaatgaatc aataccagtt tcttctaatt aattatcata ttattattgg
    18601 tattatcctt atgctgatca atctgatact tggttatata cctactcaat acgtacagtt
    18661 tttccttgtt ataggcacca tacttgtact gtcaatattt cttgtaagac tcgtggcagg
    18721 cacaattatt ggcagtaaat atattttttc atctcttttt cattttttat tgtacctttg
    18781 caccatcgaa atagcaccaa tattggtttt gtggaaatta attatagtgt taacaggata
    18841 ttagcaacta accattatgg attcgacttt aaaaacaaaa gaagtgttag agacctataa
    18901 gccggtaaaa accattctaa ttactcagcc accgccagca aatgcgaaaa atccatattc
    18961 agaaattgtt gaaaaatatg gaattcaaat tgactggcga gagtttacgc aggtagaacc
    19021 cattgatata aaggagttca gacgacaaag aatacgccct gacgaattcg gtgccgttat
    19081 ttttaccagc aaaaactcca tagatcattt cttcagaatc tgcgaagaga caagaataaa
    19141 aatgccgctc gatacaagat atttctgcat aacggaggca atatccaatt atctgcagaa
    19201 atttatcgtg taccgaaaac gaaaagtttt tacaggtgaa aaatctatcc acgaccttaa
    19261 aaattttctg attaagttcc gggagactga aaaatttttg gttcctacct ccaatcttgg
    19321 tgcaagggaa ctactctcat atcttgatga gctgaaaatc aactatcagg agtcactcat
    19381 aagtaacact gtatcatccg atctttctga tttgaaagat attacttatg atatgctcgt
    19441 attctttagt ccgctggctg tcaagtcgct ttatgataat ttcccggatt ttaagcaaaa
    19501 tgctacacgt ttggcagctt acggcaatag taccattcaa gcaatactcg atcagggcct
    19561 tcacctcaac ctcaaggctc ctgagcccga aacaccttcg atgcacatgg caatattgaa
    19621 atatcttcaa cagtcaaata ccgaaaattc ctgatattaa accatattca gtgcacgtaa
    19681 gttaagtatt aaaatcatac gaatttgctt gatcaggatt tcatcaacag attggagtta
    19741 gccattttaa aacagaagcc cggaagagaa gctcaaatac tgatggcaaa cccaggtagg
    19801 agtatggctg aaccaataca tgcaagatct gcttcagtat tgttattgtt agtttcaaaa
    19861 aacgcctcac cacacattgt gttgacaaag cgtacagcaa aatatcctga cgacaaacat
    19921 agtggccaga taagttttcc tggcgggagc ctagatccta cagatgcgaa tctgcagatg
    19981 acagccattc gtgaaacgta tgaagaaata ggtgtagaac ccgaaaaact tcaaataatc
    20041 ggctatttga ccaatttgta tattccggtc agcaattttc tggttcatcc ttttgttggg
    20101 tacctccgac aagaagccat ttataaaata caacctgacg aagttgatga aataattgaa
    20161 gtgcccgtaa gcatgttgat ggatgacaac aacataaaaa agaaaaatat cagaattttt
    20221 aatggcatca cacttaagga tgtaccgtat tttgacttct atggcaatac tgtttggggt
    20281 gctaccgcaa tgattcttag tgaatttagg caacttgtca gcaatattca gggaaaataa
    20341 tgtatttttg cgtatcaagt aattattttg cttttaaatt ttgccttaat ggaaacaatt
    20401 gccacttacc aacccaaaaa caagataaga ttagtaactg cagcctcttt atttgatggc
    20461 catgatgctg ccataaacgt tatgcgcaga atattacaat catccggtgc tgaaatcatc
    20521 cacttggggc ataacagatc tgtacaggag attgtcaaca cagctataca agaagatgta
    20581 cagggcattg ccattacatc atatcaaggc ggacacaacg aattcttcaa atacatgtat
    20641 gacctgctga aagaaaaagg tgcagctcag attaagattt tcggtggagg tggaggtacc
    20701 atactgccca cagaaattga agaactgcat aaatatggca ttgcacgtat ttacagcccc
    20761 gatgacggcc gccacatggg cctgcaaggt atgattaatg atttgttgga gcaatgtgat
    20821 ttcgctgttg gtcaaaatct aaacggtgaa ataaaggaaa tttcatcgaa gaatcctaaa
    20881 gccattgcaa gactcatctc agctgtcgaa aattttcctg aacaaaatac tacactcgtt
    20941 acagacctac aaaaagaaaa tacaccaaaa cacattcctg tacttggtat taccggtaca
    21001 ggcggagccg gaaaatcaag ccttgttgac gaactcgtca ggagattcct tattgacttc
    21061 aaagataaaa caatagccat cctgtcagtt gacccatcca aacgtaaaac cggaggagct
    21121 ttgcttggcg acagaatacg gatgaactcc atcaatgatg gccgggtata tatgcgcagt
    21181 cttgccacac gccaatcaaa tatttcactg tctaaacatg tgcattcggc tatcgaagta
    21241 ttaaaagcag ccaattatga ccttatcatt ctcgaaacat caggtatcgg tcagtctgat
    21301 acagaaataa cagattattc tgacgtggca ttgtatgtca tgacaccaga atatggagca
    21361 gcctcacagc tcgaaaagat tgatatgctt gattttgccg acattattgc catcaacaaa
    21421 tttgacaaaa aaggagcaat ggatgctctg cgtgatgtga gaaagcaata tcagcgcaac
    21481 cacaaattat ttgacaaaaa gccggaagaa atgcctgtgc tgggtactat cgcttctcag
    21541 ttcaatgacc cgggaatgaa tgcactttac aaaaaaataa ttcaaacgct ggcagaaaaa
    21601 acacataccg ggctattgtc acaaaccgaa ctggaaggag gcataagtga aaaaatatac
    21661 atcattccac cggcccgggt gcggtatctg tcagaaataa cggacaacaa ccgacattat
    21721 tcacagtggg tgctacagca ggcagatatt gcacgtacct tattcagtat taaaaataca
    21781 atagaggcag ttaaaaagca aaacctaccc aacaaagata agatactcga agaccttgag
    21841 aaaacttatc atgaaactga cataaaattt gacggtacct gccgaagaat tatccaggaa
    21901 tgggaagcaa aaaaagcatc gtatgctgca gatgaatata tctacaaagt cagagacaaa
    21961 gaatttcggc aggcgacatt cacaaattct ctttcgcaca caaaaatccc aaaagtagta
    22021 ttgccccgat ttaaagactg gggagaaata ctgagatggg tgcttgaaga aaacgtgccg
    22081 ggcgaatttc cttatacagc aggtgttttt ccattcaaaa gaacagaaga agaccctaca
    22141 agaatgtttg ccggagaagg tggaccggag cgaaccaaca cacgttttca ctatttatca
    22201 gttgatatgc cggccaatag actctccacg gcttttgaca gtgttacact ctatggcgaa
    22261 aaccctgatt tcagaccaga catttatggt aaaatcggta actcaggtgt atcggtttgt
    22321 tgtcttgatg acgccaaaaa actttattcg ggatttgacc tttgtgaccc gaaaacttca
    22381 gtttctatga ccatcaatgg tccggcagct actatttgct cattctttat gaatgctgct
    22441 attgaccaac aatgtgagaa atacatctac gagcataaac ttgaacatct tgtagaagaa
    22501 aaactactcg aactttacga aaacaaagga ttaaaaaggc caaaatataa tggagacctg
    22561 cctgaaggtc ataacggact cgggctgatg ctactcggca ttacgggtga taaggtattg
    22621 cctgctgatg tatataacca acttaaagca aaggccctag cagcagtgag gggcacagtc
    22681 caggccgata ttcttaaaga agaccaggca cagaatactt gtatattctc cactgagttt
    22741 tcactaaagc tgatgggcga tgtgcagcaa tattttataa acaacaatat caaaaacttc
    22801 tattctgtat ccatttccgg atatcatatt gctgaggcag gcgctaaccc tatcacacaa
    22861 ctcgcattta ctctgtccaa tggatttaca cttgtagagt attacctgtc gagaggaatg
    22921 gatgttgata gttttgcacc gaatttttct tttttctttt caaatggggt tgatcctgag
    22981 tattctgtga taggccgtgt ggcaagaaaa atatgggcta aagccatgaa ggaaaaatac
    23041 aaggccaatg accgaagtca gaagttaaaa tatcatatac agacatcggg taggtcattg
    23101 cacgctcaag agatatcatt taacgatata cgcaccacac ttcaggcatt gtatgcaata
    23161 tacgacaatt gcaactcgct gcataccaat gcttatgatg aagccataac tacacctact
    23221 gaagaaagtg taagacgtgc agtagctata caaatgatca ttaatcatga actggggctt
    23281 gccaaaaatg agaatccact tcagggtgct ttcattattg aagaactgac agacttagta
    23341 gaagaagccg tttataaaat atttgatgaa ataacagatc gtggcggtgt tttgggcgca
    23401 atggaaacaa tgtaccaaag aagccgcatt caggaagaga gcctttacta tgaatcttta
    23461 aaacactcag gagaatatcc gatagtaggt gtcaacatgt atttgtcaag agacggttcg
    23521 cctactatta tcccgaaaga agtaatacgt gctactgagg aggaaaaaaa gaaccaaata
    23581 gcatcgcttg aagcattgca tgacgcaaaa aaagatatct ctgctgatgt acttcataat
    23641 ctgcaaaaag cagctctcaa taacgaaaac atatttagtc atctgatgga agcatccaaa
    23701 acctgcagcc ttggtcaaat aaccgatgca ttgtacaggg taggtgggca gtacagaaga
    23761 aatatgtaga aaggccaaat acatgataat tgtaggaaaa gtgaaatgaa ttcatttttc
    23821 aaaaaaacat taactctaca aaaatgataa aagcaaacga cccgacaaga atatcctggg
    23881 tgcaatatga cggttctact gattttcctg tacagaatat tccattcggc attatgatag
    23941 caggcggtaa gcacaaggta tgttccagga ttggtgatta tattattgac atagcagctt
    24001 tatatgagct tggttttttg aatggtatag atcttggact acagcatcta gagaatgaat
    24061 ttctgaatga ttttatttct cttggcaagg aagtgacgaa tgctgttcgg gaccgattgg
    24121 ccgatgtgtt ggatgataaa aatacactgg gtgcaaaagc taaaagcgaa aaatttttac
    24181 gcccggcact tgagacggta atgagtatgc ctgtcagaat tggtgattat acagattttt
    24241 actcgagccg tgagcatgct ttcaatgttg gctgcatgtt cagagaccct aagaatgcac
    24301 tcatgcccaa ctggcttcat cttcctgtcg gttatcatgg tcgtgcttcg tcaatcatac
    24361 tatctgactg cgatgtcaag agacctaaag gacaacaact accacctgat agtgaagtac
    24421 cggtatttgg cccctgcaaa cagctcgatt ttgagcttga ggtggctttc attgtaggaa
    24481 aaagcaacca tcttggcgaa acaatatcaa ccgctaatgc agaagaatac atttttggtt
    24541 tgtgtctgtt taatgattgg agtgccaggg atatccaacg ttgggaatat gtgccgcttg
    24601 gaccgttttt atccaaaaat tttgcttcca ccctgtcacc atggatagtc accttggaag
    24661 ctctcgagcc attcaagaca gtcggtcctg ttcaggaaac gaaaattttg ccttacttgg
    24721 agtatagcgg agagaagaat tacgatatac atctgcaagt gagcatcaaa ccggagggct
    24781 cagacccggt agtagtgtca aggtctaatt ttaaatacat gtactggaac atggtacagc
    24841 aagtagccca tcatacagtg aatggctgta atctgagggt aggagatctg tgtgcatctg
    24901 gcacaattag cggccctaca ccggacagtt atggatcaat gctggaaatt gcctggaaag
    24961 gcaccaaacc ggtaaacatg aatgacggta caacaaggtc atttatactg gatggcgatg
    25021 aagtagccct taccggatat gccgaaaaaa atggtgtgcg tatcgggttt ggtgattgta
    25081 aagcaaaaat tctgccggca gattgatgac attttacaat tacatccatt taaagggtag
    25141 gttgagctca ataattatcc ttacatcttt gctcttttta tcgattgata ctgtgctggg
    25201 gcaaaatgcg cttcagcaga ctatatataa tatagcactt gatgaggatt tcaggcatgg
    25261 ttctctgagt atatgtatca tcgacttgca aaatgattca atggtagcct cttaccagcc
    25321 aaatactagt ctggtaccgg catcaactgt taaaattttt accacctatg ccgctcttgc
    25381 aatacttgga caggattttc ggttcaagac tgagttacag tatggtggcg aatttgttga
    25441 ctctacactt aatggtagtg tctttattaa aggctggggc gatccaacac ttggttcggc
    25501 aaggttcccg cgtgtggata atattaaggc acttaaccga aagtttatta aggctctgca
    25561 aaaggaatgt atatacaaaa tcaacggtta tatcgtatca gacggcagtt attacgaacc
    25621 gtcgcctgtg cctagtacct ggcagtatga tgacattggc aactattacg gggcagggtc
    25681 atttggcctc aattttcatg acaatgaata tacgctttac ttcaagcaaa atcctgaaat
    25741 gtggagcagg ccggtaatag agagaacctt cccaacgatg ccagaacaaa aatttattaa
    25801 taacgtatcg tcggcttcac ctcgctcagg tgacaatacc tatatatttg gtgatccgta
    25861 tagcaatgta cgtttggtta caggtaccat accttccgga aaaggtcatt ttgcagtacg
    25921 tggctccatt cctaatccac ctcttacatg cgccaatcgg ttggtaaaag atttttcgac
    25981 gattgattca agtagtgtga caaagggagc aattgatatt gaagaatata tcaaaacaat
    26041 aggtgcgccg ttattcagac atacatttta tacacattat tcgccaaggc tttcagatat
    26101 cattttacag actaaccacc gcagtgttaa tctatatgca gagacacttt tgcgcgagct
    26161 cgggaaaacc ttgcatgctc aaggaacagt agatgctggc atcaaggtat tgtatcagtt
    26221 ctggaaagat gcaggtctca atacccacgg attgtttttt gaagacggta gtggactctc
    26281 agcaagaaat ggagtgtcag cctggcattt tacacaagcc ctctccatag ccagaaaaga
    26341 tagccttaac ttccagacct actacaactc actacccgtt gcaggaaagg aaggcacagt
    26401 aaggcgtttg ctcaatggtt ataaaggtaa agcagaagtg agagtgaaaa gtggtactat
    26461 gcgtagagta aaaagttatt gcggctatgc aaaggcagaa gatggcaaag aatacgcttt
    26521 tgcatttatt gccaataact attcatgttc gtcgggtaaa gtgagggatc aggtcgaaaa
    26581 tattctgagg gcactcatca tatcaccaaa aaaagattga cggaaaaagt ttttctttat
    26641 ttttgtcggt gataactcaa ccacatgtat tgtagattgt tattattagt ttgtatcatt
    26701 tcgtttgttg catgccgcaa tgaacaatca aaccataaga acgcagaata tcctaatgtt
    26761 gctgattcga ctgtttcaga aaatttgcaa gaatcaaagg atcgggatat ttggcaaaac
    26821 cctgactaca tcataggtac attcggtgac ctgacagaca agacagtagc tgaccttggt
    26881 gccggttccg gatatttttc attcaaactt ttagaaaagg caggaaaagt cattgcactt
    26941 gatatagata agcattttat acagtacatg aatcaaaagg ctgcagaatt gcctcccgtg
    27001 ttgagaaata aatttgaagc aagactcgta gataccaatg acccaaaact caaagaaaat
    27061 gaagcacaga tagtattatt gtctaatact tatatctaca ttcaggacag aatcaattat
    27121 tttaaacggc ttcgcaattc tctgtcgcct ggcgggaaat tgtttgtagt agaattcaaa
    27181 aaggaaaaac ttcctgtcgg gccaccagaa tccatcaaac tgtctgccgg gcaagtttca
    27241 gaagaactca cacaggcagg ctttagtaat attaaagcag acagtagcag gctaaggtac
    27301 cagtacatcg taatggcaga aaacctgaag taaagaacat tgttgatgaa agatagattt
    27361 ctgcccgaaa ttttaagttt ttaataacta tattgcttac gggtcattcg ttataacact
    27421 agaaaatata attatgaaat atttattgac attgataatt gtaatggttc tgggaactgt
    27481 gagcttgata gcacaggcaa aaaaaggcac ctacaagatg aattccgaaa catccaagtc
    27541 taagtcggct tcaaaacttt atgaacgagg cagtaccatc aaagatgtta gtctccttac
    27601 gcagctcagc aatacaaatt cgaaatttta tattgccccg tacgagcatg gttatgttta
    27661 ttctgaagaa aagaaaaagt caaattccca ggcagctgat tatagttttt attttgttgg
    27721 tgtaagcgga aatcgttttg acaaaccgaa gacatttgct ctaaatgcgc cgacagggtt
    27781 gaagcctgtg gcagtcagct tcagcaacga caggcttacg atgttcgcta cctgtcttaa
    27841 gcccggatca aagaccagct acaccatata tcaggcaaat aaacagaata atgtttggtc
    27901 aggctggcag gagctcgaaa tagcaaagcc atttgaaagt gccggttttg cggtagtgaa
    27961 tactgcaggc aacaagatgt acctatgtgc caaatctaaa atttataaaa caggatatga
    28021 tatttataca tcagaataca atgatgggtg gcaaaaagca atattagtac aaggtgatat
    28081 taactcggac ggtgatgata tgttcccgac attgtttaat gatgtactgt tttattcatc
    28141 tggtgcaaag ggtggcgaag gcggtaagga cattattttc gtaaatctaa aagacaataa
    28201 ccgagagtgt tttaatgcag gcaataagat taataccaat gcgggtgagc aaatgatgat
    28261 tctgaacgaa gacaataaaa gcggtctgct gataagaaat gcgccaatcg ctacaaaagc
    28321 tgaagttttc aggattgtgg cagatgattt atttataaaa taacgagcaa gtgagaccta
    28381 ttttacgata aggctttcgc gttcgggccc tgtagatatc attgcaaatt taatacccag
    28441 actttcttca agtgtatcga tataggctgc agcttgttgg ggcagttgtt cgtattcttt
    28501 gatgccggct gttgaagatt gccatccgga gtatgttttg gttatggggg cgatggctac
    28561 atcatttatt tcatacggta tttcggtagt cacttttcct tcaatattat aagcatcaca
    28621 aacttcgatt tgttccagac cctgcaaaac gtctattttg gtcataacga gcttagtgac
    28681 gccattgagc ataatggtat atttgagctg tacgaggtct atccagccac aacgtctggc
    28741 acgacctgtt gtagctccga attctcctcc tttttgtcta agccattcac cttgttcgtt
    28801 gtcgagctcg gtcgggaatg ggccactacc tactcttgtg caataggctt tggtgatacc
    28861 gattacatca cctatacttg atggcgctat acccagacca atacagacac cagatgtgag
    28921 tgtattggac gaagtaacaa aaggataggt gccaaaatca atatccagca tagaaccttg
    28981 tgcaccttcg gcaagtattt ttttaccttg gttgagctgt tcgttaatat aatattcacc
    29041 attgacaaaa cgaaatctct taaggaattc tatactcgaa aaccatttct tttcttcagt
    29101 ttccaggtca aaatccactg ccgggaaaga tttgagtaaa tcgatgtgtt tctttttcag
    29161 gatttcatat ttatccttaa aatcggctga gaatatgtca cctgcacgca gaccatttcg
    29221 tccggtctta tccatatatg ccgggccgat gcctttgagt gtagaaccaa ttttgttttt
    29281 tccctttgag agctccgaag ctgcatccag atatctgtga gaaggaagta tcaggtgggc
    29341 ttttttggat ataagcagac gcgatacgta gtcaattcct gcatcatcaa gagttgtagc
    29401 ctcgcgctcg agtgttatgg ggtctattac aacaccatta cctatcaggt taatcaatcc
    29461 gggtctgaat attccggaag gtatagtatg gagcacgaat tgtttattat cgaatttgag
    29521 cgtatggcct gcatttggtc caccctggaa tctggctacc aaatcatatt gaggggcaag
    29581 gaaatcaaca atttttcctt taccttcatc gccccattga aggcctagta ttacatctac
    29641 tttcatgtat gtttgttaat ggaatgcaaa aataacaaaa ccctgcttat tgtattcata
    29701 aacagggcat tgaaatcata aatgagagta ttttcaggct gtaacagcca ttgaagcttc
    29761 ttctaagccg ttggtgaact taaaaaacgg ctttagtttt gtaatttcaa ataatttaat
    29821 cacctgagga gagacacctg aaagtgaaaa ctggcctccg actttttggg tcttgtgttg
    29881 aatggacaac agacagttga gtccggcact gttgatgtaa gggacttttg aaagatcgat
    29941 gataaagtta atttggccat tttcaataag ctcagataca gatgtaataa tctgtttgtt
    30001 ctccaattcg ttaaacagtt ggtcaacgaa taagaccttg gcgttaccgc gctccgagaa
    30061 gtggtagtta gtgttcatgg aattaaaaat ttttcggttt ttagaaattt gtgatatgtt
    30121 caatatttag ttcatgctgt attatttttt tttattttcg gatgttttct tgtgttcaca
    30181 gtcacctgta cattctccgt acaaggtaag cgaatgatga atcacccgaa attttaataa
    30241 ttcgcccatc atatctttta tctgctgaat ccgagggtca caaaattcaa ggactttgtt
    30301 gcagttgata cagatgaggt gatcgtgctg tttgtacccg tacgactttt cgtattgggc
    30361 tagattttta ccaaactgat gctttttgac aagatcacat gataccagta actccaatga
    30421 attataaaca gtggccctgc ttactctgta gtgtttggtt ttcatgtgta tgtataaagc
    30481 ctcagcatca aaatggtctg tacgcttata tatctcttct aatattgcgt atctctcagg
    30541 agtttttcta agcccttgtt tttcaagatg tgctgagaaa attgatttta cttcttcgag
    30601 aattttttcg ctaaacggtt gttcagacat ttattacttg ttatttcaac tgttacgctc
    30661 agacaaagtt agtctaaagc aaattaattt tccacaattt ttatgtatat tttatttttt
    30721 aatacatttc tctatataaa ccattgtgta gtacagtgga taaaagctta attatcaggc
    30781 ttctgcctta taaaccgatg aaataccgtc caaacctttt aaagctttga tagccattac
    30841 cagataattt tcagacggaa caacgatgct tatccttcct tcgaagtatc cttcattgcc
    30901 atctatataa aatgagcgga tattaagacc taactttcct gaaatattct gggtcagtct
    30961 ttctattaca cccgggccac tgtctattcc ggtaataacc aattcagca
//
LOCUS       DAOQQY010000008        27361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_522_length_27361_cov_6.056178, whole genome shotgun sequence.
ACCESSION   DAOQQY010000008 DAOQQY010000000
VERSION     DAOQQY010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 27361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27361
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_522_length_27361_cov_6.056178"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..866)
                     /locus_tag="PLE23_01080"
     CDS             complement(291..866)
                     /locus_tag="PLE23_01080"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMX81886.1"
                     /translation="MTKNILFLLIILCGGFPVMSQELKSTEAVPVQRYMYETARSADQ
                     INGQYPYDIRLKTPQGDSLLSADVFKKGKPIVLLFWLTTCYPCRMEMDAIFQKFDNWK
                     KETDFEIYAISTDFSQNFPAIQKRAADRKWPFSLLWDYNREFKEILPGGLNGLPQVFV
                     LDKNGKIAYHKRKYSTGDEDVLYEQVKALAR"
     gene            complement(1164..2186)
                     /locus_tag="PLE23_01085"
     CDS             complement(1164..2186)
                     /locus_tag="PLE23_01085"
                     /inference="COORDINATES: protein motif:HMM:NF025912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX81887.1"
                     /translation="MRLTYFSFIVVSLTLSGIVACKSDKKVPDTVIQTTNNPLLDELN
                     QKIKNNPKDHKSLYERAEIFYEKEAYDESIADLLDAIKIDSTQPGYYHLLADNYLDYY
                     KSRQALETMEKVAELFPKRIPTLLKLAEFQLILKRHADAQLTVQRILQVEPLNAEAYM
                     MGGMIFKDMGDQDKARAGFQKAIELGLVKDDDKIDAFINLGQLNIKKDKKLAEKYFDN
                     AIAIDSTNVHALHAKADFFQETVQYDKALEVYRKIVGIDPTYSEAYFNTGILYIDMDS
                     LGKALNNFEIATKVDPTEAVYYYYRGFVKEKMGRKDEAKQDYEQALKFDSKLEQAKSA
                     LAKFKK"
     gene            2608..3726
                     /locus_tag="PLE23_01090"
     CDS             2608..3726
                     /locus_tag="PLE23_01090"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HMX81888.1"
                     /translation="MRIGVPKETKAGEKRVAISPDVVKSLIKAGFQCHIESDAGLNAS
                     FTNDAYTAAGANIVSGAKEIYGNSDIVLKVNAPNAEEASQLKEGICLVSFLYAYTIPE
                     VIDILNQKKITAFAMDAVPRISRAQKMDALSSQANLAGYKSVILGANEMGKIFPMLMT
                     AAGTITPSKVLIFGAGVAGLQAIATAKRLGAVVEVTDVRPETKEQVESLGGKFLTVQS
                     GAEVKIEGGYAKEVSKEFLDAQQALIKKHIKDADLVITTALVVGRKAPVLVTEEMVKT
                     MKPGAVIVDMAVESGGNCEISEKGQTVSKYGVKIVGETNLPSLLPFNASELYAKNIAT
                     FLIHIANKDGFIWEAEEEIAKGSLITKDGKSVHPSIVK"
     gene            3757..4065
                     /locus_tag="PLE23_01095"
     CDS             3757..4065
                     /locus_tag="PLE23_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMX81889.1"
                     /translation="MTEILNFIAENRQLIYIIILSIFVGVEVIGHVPTVLHTPLMSGA
                     NAIHGVVIVGAIIVMLNLHPENTLALILGFLAVILGTLNVVGGFVVTDRMLEMFKKKK
                     "
     gene            4110..5507
                     /locus_tag="PLE23_01100"
     CDS             4110..5507
                     /locus_tag="PLE23_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMX81890.1"
                     /translation="MNSHLLEITYLIASVGFIIGLKMMGNPKTARNGNLIGAAGMILA
                     IIATIFLYPTEHEVAQIVYYLIFAGIAVGTIIGWYTAKKVQMTKMPELVSLFNGMGGA
                     CAALIGLIEYPHVMEKSGDHTGLLICIILGLIIGSVSFSGSIIAFLKLNGTLNKAIRI
                     PAYNMINNVLLLAVLAFGAYMVYSDVSITNLYLLFAASLLYGVLFTIPIGGADMPVVI
                     SLLNSFTGLAAAAGGFLYANQAMQTGGILVGSAGTILTFAMCKAMNRPLSNVIFGAFG
                     GGGAAAATDAAGGSIKDISVSDVAVLMNYSKKVVIVPGYGLAVAQAQHVIHELEKLLE
                     ERGVEVKYAIHPVAGRMPGHMNVLLAESNVDYSKLVEMDDINPEFPTTDVVFVVGAND
                     VVNPAAKTDPASPIYGMPILEVDKAKNVIVNKRSMSAGYAGIDNVLFGYDNCSMLFGD
                     AKKVITELVSELKNL"
     gene            6002..6139
                     /locus_tag="PLE23_01105"
     CDS             6002..6139
                     /locus_tag="PLE23_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81891.1"
                     /translation="MRSKIEATLEALHDTPKTTIFYTHVFMRARLYPLHHKSKTLGVG
                     Y"
     gene            complement(6299..7348)
                     /gene="recA"
                     /locus_tag="PLE23_01110"
     CDS             complement(6299..7348)
                     /gene="recA"
                     /locus_tag="PLE23_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMX81892.1"
                     /translation="MENNKEKEAKLKALHMAIDKLEKEHGKGTVMKLGEKKVLDVDVI
                     STGSLGLDIALGVGGFPKGRIVEIYGPESSGKTTLAIQAIAECQKKGGIAAFIDAEHA
                     FDRTYAEALGVDTENLFISQPDSGEQALEITENLIRSGAIDIIVIDSVAALVPRSELE
                     GEMGDSKMGLQARLMSQALRKLTGTIGKTGSCCIFINQLREKIGVMFGNPETTTGGNA
                     LKFYASMRLDIRKSGSAIKDKEGNVTGNHVKVKVVKNKVAPPFRVAEFDIMYGEGISK
                     TGEIVDIGTELNILEKSGSWFSYSGTKIAQGREAAKQFMADNPEVALEVEQKIKEKVA
                     AGLMKTSASVTEDMD"
     gene            complement(7470..8342)
                     /locus_tag="PLE23_01115"
     CDS             complement(7470..8342)
                     /locus_tag="PLE23_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX81893.1"
                     /translation="MEQKEFEESLKIIQNYKTDESSGIAYVDVGEGGQTLLFVHGLGS
                     NRFAWVNNVVELSKHHRCIFIDLPNYGDSEKGDFSFSLTFFKDRIYKFCYSLGLRNVT
                     MVGHSMGAQIAVKAGVEKPSFIGKLILLTPAGIETFNEFEKQWLRQINIPEILFNAPE
                     EQIRKNFEINFERFGIEAEWLLLERLEFMKDKKAYFKYCKMICKCVDAMLNEPSSNVL
                     HQLIQPTLVVFAEEDKLIPNRMLHSALKYNDLMAVAERKIINAEVATIKNAGHFAQID
                     NYREVNRIILNFLN"
     gene            8602..9342
                     /gene="phbB"
                     /locus_tag="PLE23_01120"
     CDS             8602..9342
                     /gene="phbB"
                     /locus_tag="PLE23_01120"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HMX81894.1"
                     /translation="MATKRVALVTGATGGLGTHMCKKLYDDGFLVCGNYRSREKAEKW
                     NEEMMAQGYNVYLYPADVSDFDQVGDMIRAIEKDHGIISILINNAGITRDGAFRKMSR
                     EAWDAVLDSNLGSVFNCSRHVINQMIEQNYGRIINISSVNAQRGQFGQTNYSAAKAGM
                     HGFTKTLAMEVASKGITVNTISPGYIATDMVMAVAEPVRNQIISGIPVGRLGGTEEIA
                     YLVSFLASEHAGFITGANYAINGGQHVY"
     gene            complement(9336..11108)
                     /gene="lnt"
                     /locus_tag="PLE23_01125"
     CDS             complement(9336..11108)
                     /gene="lnt"
                     /locus_tag="PLE23_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HMX81895.1"
                     /translation="MNRTLRIVFILLCAVMLVYSINQMILRPMWGYMTFLFYVSLFFL
                     FSFALAPTMLGTKASKKYFILSTAGACLMSLGYPPLATAPIILIGFVPFLILEDIISR
                     KNTGLHRAEVFKYTFSGFLLWNILTTFWVSNSALMAGFVAISLNTLFMSLTFLAFHVT
                     KVKLGKPAGYLSLVVYWLSFEYLHHHWELSWPWLTFGNSLAGYPSLVQWYEFTGTTGG
                     SLWILLSNMLLFLGFKSWLDNKQYGKGFFLGWILMVAIPIFISLNIFNHYTEKGVPQD
                     VVVVQPNFEPFYEKFTVPEKIQLTRFISLSKSKLDTSVNYLVYPETSFESVNANALNS
                     DRNIMWLERMVDEYPHLDLITGIGSYRVFDSHTVHPVTVREKVLDSGDTIFYDHHNAA
                     ISIRSGVDTIPIYFKSRFVPGAEIFPFKQLLFFLRPLVDKLGGSTEGLTAQPERSVFN
                     NGGNLNVAPVICYESIYSEYCTEYIRKGANAIFIVTNDGWWDDTPGYRQHLVYGALRA
                     IETRRSIARSANSGCSAFINQRGEIEQPTQYGVQAVIRQKILFNDEITFYVRYGDLIS
                     KAAIGISLTLVIAFLIVFFKKKNQ"
     gene            complement(11115..13259)
                     /gene="metG"
                     /locus_tag="PLE23_01130"
     CDS             complement(11115..13259)
                     /gene="metG"
                     /locus_tag="PLE23_01130"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF001100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMX81896.1"
                     /translation="MFDKKRFMVTAALPYANGPIHIGHLAGAYLAADIFVRFLRMMGK
                     DVVFVCGSDEHGAAITIKAKKEGLTPREIIDKYHVMFENTFKSIGISFDIFHRTSSEL
                     HHQTAKDFFMNLYNKGQFEERESEQYYDEEAHQFLADRYITGTCPKCGFENAYGDQCE
                     KCGSTLNPTDLINPRSTLSGSTPVLRKTSHWYLPLDKYQVWLKEWINTGKLDGAELHD
                     PATWKPHVLGQCNSWLDGGLEPRAMTRDLDWGVDVPSEIPGSDGKKLYVWLDAPIGYI
                     SATKQWAADHNRDWEPYWKDNDTALIHFLGKDNIVFHCLIFPGILHAHGEYILPVNVP
                     ANQFMNLEGNKISTSRNWAVWVHEYLEEMPGKEDELRYNLIKNMPEQKDSEFTWKGYQ
                     EVVNTELVNNLANFFNRVVVLTNKYYGGEIPDFDMEESFFSSSDPDMDTYFDSDIIHL
                     YDMLSELHGHVMSFDFRASLKLVMEISTFGNQLLQLNEPWKIQKSEPERVAVVMNFAL
                     QITAVLAVVTRPFLPFASARMHEVLGMDFSEENGELVDIMGKLAEGEAILPAGHKIGE
                     PIHLFTRITDEQVEIQIAKLENSKSASTEVALADTFVPLKPEISYDDFARMDIRTGKI
                     IKAEKMEKADKLLVLTVDLGFEERTIVSGIAKHFSPDDIIGKQVTVLANLAPRKLRGV
                     ESKGMILMAENAEGRLMFINPDEDFPAGMTIQ"
     gene            13648..13995
                     /locus_tag="PLE23_01135"
     CDS             13648..13995
                     /locus_tag="PLE23_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMX81897.1"
                     /translation="MSHSNKENKGIYKQMVFRKGLFQIKNLKDNKVFLKTSSDLDRAF
                     NSDSFQLRLGSHRNTALQQDWNSLGQDNFEFSILDELNTDENHTDKDIKKELKQLHEL
                     YIAECNKEGIVLY"
     gene            complement(14055..14615)
                     /gene="pnuC"
                     /locus_tag="PLE23_01140"
     CDS             complement(14055..14615)
                     /gene="pnuC"
                     /locus_tag="PLE23_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HMX81898.1"
                     /translation="MYYAEIIASFFAVIYIFSAAREQYISWWWSVISSLFWAYASFVY
                     FRLYVDVILQIFYIGIAFVGMYHWQRDKNKRDVLPITRMSINQHLLVIVPGFCLAYIF
                     GYIFAKYTSAASTYIDSLTTVFSVFNTFLLVRKKIENWIYWMIIDAVMAYLFFSRGGY
                     YFAALYSFYVFMSIDGYLKWRKKLFA"
     gene            complement(14694..17174)
                     /locus_tag="PLE23_01145"
     CDS             complement(14694..17174)
                     /locus_tag="PLE23_01145"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX81899.1"
                     /translation="MNRYILFFCFLIAGLSTTLAQPLNRSTARTNLSIAESRYDSMDY
                     YNSLEYYTKYLDDNKKDLPTQAKIADLNFILRDYNKAANFYSKVLNKDKKGEYSQLRF
                     NYAKALKMNENYEDAILQFSEFANSTTDPVLKKMAEIEIKGCQMAPKMEQNSRLTVTN
                     LGKAINKATTEASPYITPDGTQLFYASFNSDSAVALDGKNTDYFLKIYSSKKDGDKWA
                     PGELMNDQINREGYSTGNATFSRDGKRMYFNRAVLKGNVLSESKIYVATLGPDGWGAP
                     KEVAGVNGDFQARHPMTGELFGKEVLFFSSNMAGGHGGYDIYYATYKADDVYTDPVNL
                     GTAINSIGDEFTPFYKDGKLYFSSDSYPGLGGLDIFYSTWTGTGWTAPVNMGKGYNTS
                     VDDWYFTIDDAGYNGFLVSNRPGGRSLKSKTCCDDIYSFNIEKFKATVNTLVFDASSG
                     KVVQQYNAQLIEMTGDKMGVTESKNSGKTNTVDFPLVLEKSYAMIINKDGYYPDTIKG
                     INTVALAKDEAFKYKVNLKPKEPEYEIYSTEEAIRLNNIYYDYNDDKILPSAEGDLTL
                     IYDLMKQYPDMVIELSSHTDARGDDDYNMKLSQRRANSAKNWLVKKGIAGDKIQAVGY
                     GETKILNKCVNDIECTDDEHRFNRRTEFKIVSGPTSIKIEKKRLKGSKEEGVIINKTG
                     DMPKVAPKEEPKSDSKGTTAASEKEPKLKPTKSYSGPSLLRFDPTAHNYGVIPYKDKR
                     SFTYQFTNVSDQDVEIDLCSACECMTLDWTKGVIKPGGKGTIKAVLDTTPKSKGEDVD
                     DSINVMLNTVGDKSVPFIYTVGYKAKVK"
     gene            complement(17204..18226)
                     /locus_tag="PLE23_01150"
     CDS             complement(17204..18226)
                     /locus_tag="PLE23_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMX81900.1"
                     /translation="MRFLKLGTILICLFVGNILMAQDIHFTQYNMSPMTLNPALTGNY
                     LGSYRVGGIYRNQWASIIPAYSTPSFFVDAPIITGFRKNDWIGVGVAITADKSGTAKL
                     GRTAFMGSVAYHLGLNKKSTSVLTIGLQGGRQSQNVDLQSDKIKFIDELESPIPIQSA
                     DRNLGQGDKKGYLDINAGLTLKSKLNQQMDFIVGFAMGHINKPEYAFSGDKGSQNRLS
                     HKYVGHGTFNVNLNPVWSISPSFLYQTIAGQNEMVLQGLAGYKLKNTKAKVQLNFGLG
                     YRLRDAAEILIGMDYDNLKVGFSWDANTSGVSGKFNGGYEIAAMYTGMIYKKPKVNTV
                     IFCPRF"
     gene            complement(18311..19333)
                     /locus_tag="PLE23_01155"
     CDS             complement(18311..19333)
                     /locus_tag="PLE23_01155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMX81901.1"
                     /translation="MKVLIIRLSSIGDIILTTPVVRCIKQQLGAEIHFLVKKSFANVL
                     AGNPYIDQIHYYERWDEEAERRLREEQFDHIFDLHKNLRSKQIIYSLKLPSTDFDKLN
                     VRKWFMTALKINILPKKHIVDRYFEALERVGLKYDGKGPDFFISDENITDADRFLNLK
                     GIPDGASYVAMAIGAARKTKLPTIDLYIQIINRLGLPVVLLGGPGDKQFGEEIANSMP
                     ASVIVNAAGELTLQESVAVLKRALCLVTPDTGLMHAGAAMGTPMVVIWGNTIPEFGMY
                     PLYKDNTDAVYHNHEVYGLSCRPCSKIGYEHCPKKHFHCMKQQNMVLIVKNIESLINS
                     RQKKHN"
     gene            complement(19396..22509)
                     /locus_tag="PLE23_01160"
     CDS             complement(19396..22509)
                     /locus_tag="PLE23_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HMX81902.1"
                     /translation="MKKIAWIMLLVFSVSAIYAQSVKKEVNRNPLLKVDESNRGEFKA
                     YKAMVPGAKAQFTESEPEYRSMGGLPVFAKYHDGPKVKMYSEDGLPVWVEGRLPGSVV
                     LPVDARQQAMTYLQFMGRQMQIKEPMTEFALSEDETDELHIRHITYTQMLGGIEVFGA
                     EVKVHTDADGPYLMNGRYFPTPDLTSLTPAVSEVQAIQNVKNDVSRLTTLKNLTDQEL
                     KLVKDHVQVKSKLVIYHKNNDAHAERLAWHIEIYPNVAHRYEYFVDALTGNIIHSYNT
                     ICGLHNQMHHSGECHAAVMNPLDGPATANAIDLNNKPQLLNTYQAGNKYYLIDITKPM
                     FKPAQSNLPTDAVGVIATLDGLNKSPENSGFTVADVTSTTNTWTDKSSVSAHYNGAKS
                     YDYFLATHNRNSIDGSGGNILSLINIADSDGGGLDNAFWNGQAMFYGNGKIGFTPLAG
                     GLDVAGHEMTHGVVEKSANLVYQNESGALNESFADVFGVMIDRDNWNIGETVAKKSYF
                     PTGIMRSMSDPHNGGNSLNDNGWQPKHMNEKYNGGEDNGGVHINSGITNHAFYLFATS
                     PNVGKEKAEKVYYRALTQYLVKSSVFIDARAAVVQAAKDLYGNSSAEVTAAENAFAQV
                     GIGTGGSSSGDKYQKNLNPNPGSDFIVATDAIPNQVYLAHGNGFATIDVISGTTPKSR
                     FSVTDDGSAIFYVGEDENIHAIQINYSNGQVTETKVSTSPVWGNVAVSKDGSRIAMVS
                     NAVENKITVYDFNLQQGQTFTLYNPSYSGSVTGNVLYADALEWDYSGNYVMYDAYNEI
                     KNQAGSTINYWDIGFIRVFDKKKNTFGDGNISKLYSGLPENTSIGNPVFSKNSPYIVA
                     FDYIDEIENTVYLVGGNIETGKEDAISNQSDLFVPNYSRLDNYIIGTVNTNEIHAFQL
                     ASDKISYGGSSTQLFTDATNGYWFGNGTRTINVATHEPSEKIANWSVFPNLVNNSVVI
                     KAVNDMGNGVVQIVNSTGNTVRSEELNPLGKGVTQHINVSDLPAGAYFMRIMSGNNYQ
                     TEKIIKQ"
     gene            complement(22506..22946)
                     /locus_tag="PLE23_01165"
     CDS             complement(22506..22946)
                     /locus_tag="PLE23_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81903.1"
                     /translation="MRRLKILSVLMLLSAIFSCKSSHFTAENLPEKQLIIGSQGGVSG
                     FGSEYIFCESGQVFKNSTANKKTEEIRIKKKSEVRKFFKTAFKQSWMKDEYQKPGNFS
                     SFIIFKEKDIQYKFVWANNDTLVNPAVKSMVHDAKELLNKNSEK"
     gene            complement(23172..24575)
                     /gene="lpdA"
                     /locus_tag="PLE23_01170"
     CDS             complement(23172..24575)
                     /gene="lpdA"
                     /locus_tag="PLE23_01170"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMX81904.1"
                     /translation="MNYDVVVIGSGPGGYVAAIRCAQLGFKTALIEKYPSLGGTCLNV
                     GCIPSKALLDSSEHYHNAKTGFEAHGVYMKDLTADLNKMVARKAQVVLENTKGIEFLM
                     KKNKIDVHYGVGSFKDKNTVTVKATDGQTTELSAPKIIIATGSKPTVPASFNYDKKRV
                     ITSTEALQIKDIPARMCIIGGGVIGLELGSVFARLGTEIEVVEFADSIIPTMDVDCTK
                     ELTRSLKKLRMKFHLKHKVTAVHASESSVEVQVENTVKGETFSVHADYCLVAIGRRPY
                     TDQLALENAGLTTDERGRIPVNHHLETAVPGIYAIGDVIEGPMLAHKAEEDGVFVAET
                     IAGQKPHLNYNLVPGVVYTWPEVAAVGQTEAQLKAAGMAYKTGKFPYKALGRARASMD
                     VDGMVKILADATTDEILGIHIVGARAADMIMEGVTLMEFRASAEDAARICHPHPTYTE
                     AIKEAALAATANRSIHS"
     gene            complement(24611..26041)
                     /gene="ricT"
                     /locus_tag="PLE23_01175"
     CDS             complement(24611..26041)
                     /gene="ricT"
                     /locus_tag="PLE23_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HMX81905.1"
                     /translation="MSCSGCSSGGSCGSSGCGKSGGCASGGCNRLNTFDWLAALDIHD
                     PYESELVEVSFKNGSTKSFYTNPPHTRATSGDWVVVDTGNGFDVGRISLSGDLVNLQL
                     KKKNIKKDTVFNNVLRKANERDLQRMQEAREKEPEIMVKSRVISRSLKLEMKVCDVEL
                     QADHRKATFLYTADGRVDFRELIKHFASEFKVKVEMRQIGARQEAARIGGIGSCGREL
                     CCSTWLSDFKSVSTTAARYQNLAINQSKLSGQCGRLKCCLNYELDVYLDALEDFPEKA
                     DKIQTQAGPAILVKTDVFKKIMFYVNEREQGRGKFYPLAVVQVKEIIAGMKAGKLVES
                     LDEVQIATIVNEDEVEKDYEDVTGFVELPAEKRKKKKKKKPRDSREQSTPKDKPTSTT
                     NAPEQRRQDTPRVNTRQENTRQDNTLQDNTNDTGTVSGNDHANRGSNQNPNRPNNPKH
                     RQGRKFHGKPGNRPPRDKPEQGDKKE"
     gene            complement(26051..27271)
                     /locus_tag="PLE23_01180"
     CDS             complement(26051..27271)
                     /locus_tag="PLE23_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81906.1"
                     /translation="MGDVYVAVGMPGTGDAEVCRLKRKNDYLCRVMLFKDIIGQKKVI
                     ADLLSALKYGRLPHAILLSGKSGHGSLPLALALAQYVFCENPAADDACGVCHACVKTS
                     KLIHPDLHITVPAFGAEAVSDTFLPQFRKMFVENAYMDMADWIAAHDAESKQWNITAK
                     ECLNIVKKLNMQAYEGRWKVQVIWMAEYLAKEGNRLLKIIEEPPADTFFVLIAENEDR
                     VLSTILSRCQVYRVPLLHDADIYDKLLAEGVEEGEASVITRLAGGNYSDAIRLREHTV
                     GSLDEQFISWLRICFKGNAVEMNNWTDGYAALNREEQKKFVRYGLNFMHELVLLKVVG
                     KALRLSDDKAETALKIKNLLDLEQINEICRLLDECGFGLERNANIRLLMFDAAIQLKN
                     IYKKTGVVKQPLHK"
BASE COUNT         7503 a         5989 c         5460 g         8409 t
ORIGIN      
        1 gaggtgtcga ggtgtcgagg tgtcgaggtg tcgaggtgtc gaggtgtcga ggtgttttca
       61 tatttgattg gtgtttttgt ggatgattta ataaaatcaa agatacaaaa taacccgcaa
      121 ttttcaaaat atatttttct tatttttagt aaaaaattta ttttccaaat aacattttga
      181 atacaacata ttttaaagct gatcaacaga tgattgtttg gtgtgtgtag atattgccaa
      241 tatcttctcg ttcattacaa acatccatat tggatagaag aatactcatc tcacctcgcc
      301 agtgccttca cctgctcata gagcacatct tcgtcaccgg tagagtattt gcgtttgtgg
      361 taggctattt tcccgttttt atccagcaca aacacttgcg gaagtccatt cagaccgccg
      421 ggtagtattt ccttgaattc gcggttatag tcccacagca agctgaatgg ccactttctg
      481 tcagctgcac gcttctgtat tgcaggaaaa ttttgagaaa agtcggtact gatggcataa
      541 atctcaaaat ctgtttcttt cttccagttg tcaaattttt gaaaaatggc atccatttcc
      601 atgcggcatg gatagcaggt agtgagccag aatagcagta ctataggttt gccttttttg
      661 aaaacatctg cactcagcag cgagtcgcct tgtggggtct tcaaacgtat gtcataaggg
      721 tattgtccgt taatctgatc tgccgacctt gcggtttcat acatatacct ctgcactggc
      781 actgcttcag tacttttcaa ctcttgcgac atgacaggaa aacctccaca taatattatc
      841 agcagaaata gaatattttt tgtcatagtc ttaattttta attcattcga ataaaacccg
      901 gataaggcat taggttcttt actgcaaata atgattactt caaatctatt tcaaaagctc
      961 aaaaaccata gttcactacg cttttatctc ttttttatgg atttttgtgc gaatcatttt
     1021 cgcttaaaat cataatgccc tatctggttt aaagatacaa cgaaatgaag cgttaattag
     1081 tttttacagg cgcgaatttg atgaatggca tttgcgagaa tcattctttt gtatcttgtt
     1141 acattcggtt tggctgatga catttatttt ttaaatttcg caagcgctga tttggcctgt
     1201 tcgagttttg aatcgaactt gagtgcttgt tcgtaatctt gtttggcttc gtcttttctg
     1261 cccatttttt ctttaacaaa tccgcgataa tagtaataaa ctgcttcggt ggggtcaacc
     1321 ttggtggcga tttcgaaatt attgagtgcc ttaccgagcg aatccatgtc aatatacaag
     1381 atgccggtgt taaaatatgc ttcggaataa gtagggtcga tacctacaat tttacggtaa
     1441 acttccagtg ctttgtcgta ttgtactgtc tcttgaaaaa aatcagcctt agcatgtagc
     1501 gcatgtacat tggtgctgtc aatagcgatg gcattatcaa aatacttttc tgcaagtttt
     1561 ttatcttttt ttatgttgag ctgccccaga ttgatgaatg catctatttt gtcatcgtcc
     1621 tttaccaaac ccagttctat tgctttctga aatcctgccc ttgctttgtc ttggtcgccc
     1681 atgtccttga atatcatacc gcccatcata tatgcttcgg cattgagcgg ctctacctgc
     1741 aaaatacgct gcactgtgag ctgtgcatcg gcatgacgtt tgaggatgag ctgaaattcg
     1801 gcgagcttca gtaaggtagg tatgcgttta ggaaagagct ctgctacctt ttccattgtt
     1861 tcgagcgcct gccgcgactt gtaataatcg agataattat cagcaagcag atggtaatag
     1921 ccaggctgtg tactgtctat ttttatagca tccaacagat ctgcaatgga ctcgtcgtag
     1981 gcttcttttt cataaaatat ttcggcgcgt tcgtagagcg atttgtgatc ctttgggtta
     2041 ttctttatct tttggttaag ttcatctagc agagggttat tggttgtttg gataacagta
     2101 tcaggtactt ttttgtcaga cttgcaggca actatgccag acagtgttaa agaaacaacg
     2161 ataaaggaaa aataagtgag acgcatttta aaaaaatatt gattttgagg ctgtaagttc
     2221 gaacgaattt cacaattagc aaaacgatga cccacacttt gtgtgaatat tgataaaatc
     2281 tgactgtttc cagctcgata ttctaaatca agcacagcac tcattcttac atttcacaaa
     2341 aaaccgatga ttgacaggca tttattccta caatcatttc acgcaaaaag taaaaccact
     2401 ttacttctga aaagatttga aaaactacca ctattgctat tttttttaga acatctggcc
     2461 taaaaaattt aaaacacgac aaaaacccta tgatttacat caccttttca tgaatttcac
     2521 gtatatggca ttgtatttaa ttcaatttct tttctatatt tacgccctgc aaaaaaaaac
     2581 agcatagctt aactaaataa aatattgatg cgcataggag tccctaaaga aacaaaagcc
     2641 ggtgaaaaga gagtagccat atcaccggat gtagtaaaat cattaatcaa ggctggtttt
     2701 cagtgtcaca tagaatcaga tgccgggtta aacgcatctt tcaccaatga tgcatatacg
     2761 gctgccggag caaatatcgt ttcgggtgca aaagaaatat acggcaattc tgacatcgta
     2821 cttaaagtaa atgcacctaa tgctgaagaa gcatcgcaac tgaaggaagg aatatgtctt
     2881 gtatcgtttt tgtatgctta taccattccg gaggttatag acatacttaa ccagaaaaag
     2941 ataactgcat tcgctatgga tgccgtgcct cgtatatcgc gtgcacagaa aatggatgct
     3001 ctttcttcgc aggccaatct tgccggatac aagagtgtca tactcggtgc caatgagatg
     3061 ggcaaaattt ttccgatgct gatgaccgct gcaggtacca ttactccgtc aaaggtgctg
     3121 atattcggag ccggtgtagc cggacttcaa gccatcgcta ctgccaaaag attaggtgcg
     3181 gtagtagaag taacggatgt acgccctgaa accaaagaac aagtagagtc actcggagga
     3241 aaatttctga ccgtacaatc aggcgcagaa gtaaagatag aaggcggcta tgccaaggaa
     3301 gtatcgaaag aatttttaga tgcccaacag gcattaatca aaaaacacat caaagatgcc
     3361 gacctggtca tcacgactgc actggtagta ggtcgcaaag ctccggtact ggtgactgaa
     3421 gaaatggtaa aaaccatgaa accgggtgct gtcatcgtag atatggcagt agaatcgggc
     3481 ggcaattgtg aaataagcga aaaaggacaa actgtaagta agtacggtgt aaagatagtt
     3541 ggcgaaacca atctgccaag tcttttgcca tttaatgcaa gtgaactgta tgcaaagaat
     3601 atcgctacct ttctcattca catagccaac aaagacggat ttatctggga agcggaggaa
     3661 gaaattgcca aaggcagcct tataaccaaa gacggaaaat ccgttcaccc aagcattgta
     3721 aaataatata aagcccaaac taaaaatcat ttcgccatga ctgaaatatt aaattttata
     3781 gctgaaaaca gacagttgat atacatcatc atcttatcca tattcgttgg tgttgaagtg
     3841 atcggccatg tacctacagt actgcacact ccattaatga gtggtgccaa tgccatacac
     3901 ggtgttgtaa tagtaggtgc catcatcgtt atgctcaacc ttcatccaga aaacacactt
     3961 gcacttatat tgggctttct ggccgtcata ctgggcacac tcaatgtagt cggcggattt
     4021 gtagtgaccg accggatgct cgaaatgttt aaaaagaaga aataataaat caacagcatt
     4081 agataagaac tgaaataaca gaaaaatata tgaacagcca tttactagaa ataacttacc
     4141 tcatcgcatc tgtcggattc atcattggtc tgaaaatgat gggaaatccg aaaactgccc
     4201 gaaacggcaa cctgataggt gcagccggaa tgattctggc catcatcgct accatctttc
     4261 tctaccctac agaacatgaa gtagcccaaa tagtatatta tctcatattt gcaggtatag
     4321 ccgtaggaac catcataggt tggtacactg ccaagaaggt acagatgacc aaaatgcccg
     4381 aattggtttc tctattcaac ggtatgggtg gtgcatgtgc cgctttgatc gggctgatag
     4441 aatacccgca cgtgatggaa aagtccggtg atcacacagg tttgctgatt tgtatcattc
     4501 tagggcttat cattggttcg gtttcgtttt cgggaagtat tattgcattc ctaaaactca
     4561 atggtacact caataaggca atcaggatac cggcgtataa catgattaat aacgtattgc
     4621 tgcttgcagt attagctttc ggtgcttata tggtttacag tgatgtatcc attaccaatc
     4681 tatatttgct ttttgctgca tctctgctat atggcgtatt gtttaccatc ccgataggtg
     4741 gtgccgacat gcctgtagtc atttctttgc tcaattcatt tacaggtttg gcagcggctg
     4801 caggaggatt tctgtatgcc aatcaggcca tgcaaacagg cggtatattg gttgggtcgg
     4861 caggtacgat tcttacattt gctatgtgta aagcaatgaa ccgtccactc agtaacgtca
     4921 tattcggagc atttggtggt ggtggtgcag ctgctgcaac ggatgctgcc ggtggcagta
     4981 taaaggacat aagtgtatcg gatgtagcgg tactgatgaa ttattctaaa aaagtggtga
     5041 tagtaccggg ttatggtctg gcagtagcgc aggcacaaca cgtaatacac gagcttgaaa
     5101 aattgcttga agaaagaggt gtcgaagtaa aatatgctat ccacccagta gcgggtcgta
     5161 tgccaggtca catgaacgta ctacttgccg aatcaaatgt agattattcg aaacttgtag
     5221 aaatggatga catcaaccct gaattcccaa ctactgacgt ggtttttgta gtcggtgcaa
     5281 atgacgttgt taaccctgct gcaaaaacag atccagcatc acctatctac ggaatgccaa
     5341 tactggaggt ggacaaggcg aaaaacgtaa tagtgaacaa gagaagtatg agcgcaggtt
     5401 atgccggtat agataatgta ctctttggtt acgacaattg ttcgatgtta tttggtgatg
     5461 ctaagaaagt catcacagaa cttgtatcgg agcttaagaa tctgtaataa ttatgacttg
     5521 accaaacaat agtcatagct tagaaatatt ttattaaaag gaagtccaaa tatattgagg
     5581 cttccttttt cattttccgg atggcgcaca tcactatcct tctatttaac aaattgtatt
     5641 actttcagaa aatttatacc taatcctgca tatagcatat ctaaagcaaa ataaaggcaa
     5701 aataaaggtc tatatactta cataaaggta aatacagctt ccaccagcac ggctatgtct
     5761 aggtaaatac aacttctacc agcacggcta cgtctaggta tatacaactt ctaccatctt
     5821 gccttgttat ttagcaaaac aaaaaagtaa cttaagtgtg cattagctca agggattaac
     5881 tccgaagaac cataaccaag gccatactta gattaaccta cgtgcttgca actacggaaa
     5941 aacttgtaca ctactccatt attgaccaag tcaatatcat tattgaatct ttatacaatc
     6001 attgcgttca aaaatagaag ccacactaga ggcactacat gatacgccta aaaccaccat
     6061 attctatacg catgtgttca tgcgtgccag gctatatcca ctacatcata agtcaaaaac
     6121 cctgggagta ggatattaga ttttacataa cttttacact tgcagtgttc ggaaagccta
     6181 aaaatttgga aagtatcgaa tcatggaaaa aaatgcacga tttcaagagt tgaagctcga
     6241 aatcatgcat tgtacaatta ccgcatgaca ggaaagcgtg tttccgtcag aagtgaattt
     6301 aatccatatc ttctgtaacg cttgctgaag tcttcatcag acctgctgct actttttcct
     6361 ttattttttg ttcgacctcg agtgctacct caggattgtc tgccatgaat tgtttggctg
     6421 cttctcggcc ttgagcaatc ttagtaccgg agtaactaaa ccacgaacca cttttttcaa
     6481 gaatattcag ttctgttccg atatctacaa tttctccggt tttagagata ccttcaccgt
     6541 acatgatatc gaattcggct actctgaaag gtggcgctac tttgtttttt actactttca
     6601 cctttacgtg attgccggta acatttcctt ccttgtcctt gatagcggag ccagacttgc
     6661 ggatatccag ccgcatcgat gcgtaaaact taagcgcatt accaccggta gttgtctccg
     6721 ggtttccgaa catgacaccg attttttctc ttagctgatt gataaatatg cagcaagagc
     6781 ctgttttacc gatggtaccg gtaagttttc tgagtgcctg cgacatgagg cgagcctgca
     6841 gacccatttt cgaatcaccc atttcgcctt cgagttcgct ccttggtacc aatgctgcta
     6901 ctgagtcaat aacaatgata tcaatggctc ctgaacgaat cagattttcg gtgatttcga
     6961 gtgcctgttc gcccgaatcg ggttgcgaaa taaagagatt ctcggtatcg acacccaaag
     7021 cttcggcata ggttcggtca aaggcgtgtt cggcatctat aaaggcagcg atacctcctt
     7081 ttttctgaca ttcagctatg gcctgaatag ctaaagtggt cttacctgaa gattccggac
     7141 catagatttc tactatcctt cctttaggga acccaccgac gccaagagca atgtcgagcc
     7201 cgagtgaacc tgtagatatc acgtcaacat ccagtacttt cttttcaccg agcttcatga
     7261 cggtgccttt gccgtgctct ttttcgagct tgtcaatggc catgtgcaag gcttttagtt
     7321 ttgcttcttt ttccttatta ttttccaact tgttacgatt ttaaatatta acagatgcaa
     7381 atataaaaca atttgtatat ttttaaatta ttttaaatat ttttttgttt tttttacaaa
     7441 gacgtttgac tccgtaaaat tccataaatt taattcaaaa aattaagaat tattctgttt
     7501 acctctcggt agttatctat ttgggcgaaa tgtccggcat tcttgatggt agctacttct
     7561 gcgttgataa ttttgcgttc ggctacagcc atcagatcat tgtatttcag ggcactatgc
     7621 agcatacggt tgggtatcag cttgtcttct tcagcaaaaa caacgagtgt aggttgtatc
     7681 agttggtgaa ggacattaga ggatggttcg ttgagcattg catctacaca tttgcagatc
     7741 attttacaat acttaaaata tgcctttttg tctttcataa actccagtcg ctcgagtaaa
     7801 agccattcgg cctctatgcc aaaccgttcg aagttgattt caaaattttt tctaatttgt
     7861 tcttcgggag cattaaagag aatttcagga atatttattt gacggagcca ttgtttttcg
     7921 aactcgttga aggtctcgat gcctgccgga gtgagtaaaa tgagcttacc gatgaaggaa
     7981 ggcttttcca cgcctgcttt cactgctatc tgggctccca tcgagtgtcc gaccatggta
     8041 acgttgcgca gaccaaggct gtaacaaaac ttatatatcc tgtctttgaa gaatgtgagt
     8101 gaaaaactaa aatccccttt ctcagaatcg ccataattgg gcaaatcaat gaaaatacaa
     8161 cgatgatgtt ttgacaattc tacaacgtta ttgacccatg caaaccgatt gctgccaagg
     8221 ccatgaacaa acagaagtgt ctgaccgcct tcaccaacat ctacataagc tattccggat
     8281 gactcatctg ttttataatt ttgaatgatt ttcagtgatt cttcaaattc tttctgctcc
     8341 attttacaaa tgtaaaccaa tcaattcaat aataatttac aaaataacaa tacaggcaat
     8401 gaatgaagca aaaattgcat aaatacaatt acgtaacaat ataattctca ttacgaattc
     8461 ggtaaagcgg tggaaaattg cggctctgcc gtgaccaaaa aaaaatatat tcaaaaaact
     8521 tatgaataat attgcacaat gtaatggaaa tgctatatat tggaatctta aattatttaa
     8581 ccaatcaaaa attaatatca aatggcaaca aaacgtgtag cattagtaac cggagccaca
     8641 ggaggccttg gcacacacat gtgcaagaaa ctttacgatg atggtttttt ggtttgtggc
     8701 aattacagat caagagaaaa agctgaaaaa tggaatgaag aaatgatggc acaagggtac
     8761 aatgtgtatc tgtatccagc tgatgtatct gactttgacc aggtaggtga tatgatcaga
     8821 gctattgaaa aagatcatgg tatcatttct attttgatta acaatgcagg tataacacgt
     8881 gatggcgcat tcagaaagat gtcgcgcgaa gcttgggatg ctgtccttga ctctaatctg
     8941 ggaagtgtat tcaactgttc gcgtcatgta atcaaccaga tgattgagca gaactacggt
     9001 cgtatcatca acatctcatc tgtaaatgct cagcgtggtc aatttggtca gaccaactat
     9061 tcagcagcaa aggcaggtat gcacgggttt acaaaaacac tggcaatgga ggtggcttca
     9121 aaaggtatta cggtgaatac catctcaccc ggctacatag ctactgatat ggtaatggct
     9181 gtggcagaac cggtcaggaa tcagattatt tcgggcattc cggtaggcag attgggcggc
     9241 acggaagaga tagcttatct ggtatcgttt ctggcttcag aacacgcagg tttcataacg
     9301 ggtgccaact atgccatcaa tggcggccag catgtttact gatttttctt tttaaaaaag
     9361 actattagaa aagcaatgac caatgtcaaa gaaataccta ttgcggcttt tgaaataaga
     9421 tctccgtatc tgacataaaa agttatttcg tcattgaaca aaatcttttg tcgtataaca
     9481 gcctgcacac catattgtgt aggctgttct atttcgcctc gctggttgat gaatgctgag
     9541 caaccactgt tggcagacct ggcaatggat ctcctggttt ctattgcccg aagggcgcca
     9601 tatacaaggt gctgccggta ccccggtgtg tcgtcccacc aaccatcatt ggtgacaata
     9661 aatatggcat tggctccttt gcggatgtat tctgtgcagt attcgctgta gatagattcg
     9721 tagcagatga ccggggcaac attcagattg ccaccattat taaataccga cctctctggt
     9781 tgtgctgtca atccttcggt actcccaccc aatttgtcaa ccaatggtcg gaggaaaaac
     9841 aacagttgct tgaaaggaaa tatctcagca ccgggcacaa accgtgactt gaaataaatc
     9901 gggatggtat ctacgcctga cctgatactg atggctgcat tatgatggtc atagaatata
     9961 gtatctccgg agtcgagtac tttctcacgt acagtaaccg ggtgtacggt atggctgtca
    10021 aaaaccctat atgaaccaat gcctgttatc aaatcaaggt gcggatattc atctaccatt
    10081 ctttcgagcc acatgatgtt tctgtcgctg ttgagggcat ttgcattgac cgattcgaaa
    10141 ctggtctcag gataaacaag ataatttaca cttgtatcga gctttgattt gctcaggcta
    10201 atgaaacgag tgagctgaat tttttcaggt actgtgaatt tttcataaaa tggttcgaaa
    10261 ttaggttgca ctactacgac atcctgtggt acaccttttt ctgtataatg attgaaaata
    10321 ttgagcgaaa taaatatcgg tatggctacc ataagaatcc aacccagaaa gaatccttta
    10381 ccgtattgct tattgtctaa ccaggatttg aagccaagga acagaagcat atttgacaac
    10441 aatatccaga ggctgcctcc ggtagtacca gtgaattcat accattgcac cagtgaagga
    10501 tacccggcaa gactattgcc aaaggtaagc cagggccatg acagctccca atgatggtga
    10561 aggtattcga atgaaagcca ataaactacc aaactcaaat aaccggctgg tttacctaac
    10621 tttaccttag taacatggaa tgccagaaat gtaagcgaca tgaacaaggt gttgagcgat
    10681 attgctacga agcctgccat caatgcactg ttcgaaaccc agaatgtggt aagaatattc
    10741 cacagtaaaa aacctgaaaa tgtatattta aagacctctg ctctatggag tccagtattt
    10801 tttctcgaaa tgatgtcttc cagtataaga aaaggtacaa atccaattaa aataatcggt
    10861 gctgttgcca agggtggata tccaaggctc atcagacatg cacctgccgt acttaggata
    10921 aaatattttt tacttgcctt ggtgcccaac atggtaggtg ctaaagcaaa tgaaaataaa
    10981 aaaaataaac tgacatagaa taaaaacgtc atgtaacccc acatcggcct gagtatcatt
    11041 tggttgatac tgtacacaag cattacagca caaagcaata tgaatacaat tctcagggta
    11101 cggttcattg acggttattg tatggtcatt cctgccggaa aatcttcatc cgggtttata
    11161 aacataagtc ttccttcggc attttcggcc atcagtatca tgccttttga ctccacacca
    11221 cggagttttc ttggtgccag gttggccagt acagtcactt gcttgccgat tatgtcatcg
    11281 ggtgaaaaat gtttggcaat acctgaaaca atggtgcgct cttcaaagcc aaggtcaact
    11341 gtcaggacaa ggagtttatc ggccttttcc attttttctg ctttgatgat tttccctgta
    11401 cggatatcca ttctggcgaa gtcatcgtag gaaatttcgg gtttcagagg aacaaatgta
    11461 tctgcaagtg ctacctcagt acttgcagat ttcgaatttt cgagctttgc tatttgaatt
    11521 tccacttgtt cgtctgtgat tctggtaaac aaatgtattg gttcgcctat tttgtgcccg
    11581 gcaggcaaaa ttgcttcacc ttcagcaagc ttgcccataa tatccactag ctcaccattt
    11641 tcttctgaaa aatccatacc aagtacttcg tgcatgcgtg ccgatgcaaa aggtaaaaac
    11701 ggccttgtaa caacagccag cactgcggta atctgtaggg caaaattcat cacaacggct
    11761 acacgttcgg gttctgattt ctgtattttc caaggctcat tcagttgaag caactgattg
    11821 ccgaatgtcg atatctccat cacgagcttc aacgatgctc ggaaatcgaa actcataaca
    11881 tgaccgtgca attctgataa catatcgtac aagtgtatga tatcggagtc gaaatacgta
    11941 tccatatcag gatcggagct gctgaaaaaa ctttcttcca tgtcaaaatc cggaatctca
    12001 ccaccatagt atttattggt aagcactact accctgttga agaaatttgc caggttgttg
    12061 accagttcgg tattgaccac ttcctggtag cctttccagg taaattcgga gtctttttgc
    12121 tccggcatat tttttatcag gttatagcga agttcgtctt cctttccggg catttcttca
    12181 aggtattcgt gtacccaaac agcccagttg cggctggtgg aaattttatt gccttcgagg
    12241 ttcataaact ggttggcagg cacattcaca ggcagtatat actcgccatg tgcgtgaaga
    12301 atacccggaa aaataaggca atggaatacg atattgtcct taccaagaaa atggataaga
    12361 gccgtatcat tgtctttcca gtagggttcc cagtcgcggt tatggtctgc ggcccattgt
    12421 ttggtagctg atatatagcc aatcggtgca tcgagccaga cgtagagttt cttaccatcc
    12481 gagcccggta tttctgaagg tacatcgaca ccccaatcga ggtcgcgtgt catagctcgt
    12541 ggttcgagtc cgccatcgag ccaggagttg cactgaccga gtacatgggg cttccatgtt
    12601 gcagggtcgt ggagttcagc accatcgagc ttgccggtgt tgatccattc tttgagccat
    12661 acttggtatt tatcgagtgg cagataccaa tgcgatgttt ttctgagtac cggtgtagaa
    12721 ccggacaatg tagaacgtgg attgataagg tcagtaggat taagtgtcga accacatttt
    12781 tcacactgat cgccataggc attttcgaat ccacacttcg ggcaagtacc ggttatgtat
    12841 ctgtctgcaa ggaactgatg tgcctcttca tcgtagtatt gttctgattc tcgttcttca
    12901 aactgtcctt tgttgtaaag attcatgaaa aaatccttag cagtttgatg atgaagctca
    12961 gatgaagtgc ggtgaaaaat atcaaatgaa atgccaatac ttttgaaagt attctcgaac
    13021 ataacgtggt atttgtctat tatctcacgc ggagtaaggc cttccttttt cgcttttata
    13081 gtaatagctg ctccatgttc gtcagagcca catacgaata caacgtcttt gcccatcata
    13141 cgcaagaagc ggacaaagat atctgctgcc aaatatgcac cggcaagatg gccaatgtgt
    13201 atcggtccat tggcataagg taatgccgca gtcaccataa atctcttctt gtcgaacatc
    13261 gctttcatat taaagcctca aagataagcg aatatcatcg aacgataatc acttgaaaaa
    13321 attaattgaa tcagaaaacc tcttgaaata aaagaagaat acataagcta cacgacacat
    13381 tcatgcaaat gttgatatat agaactatga ttttctcggg ctaaatcaaa tattattcta
    13441 tatgcactac atgcacttat tgagaaaaaa gaatctcttt tcacataaga tttattgctc
    13501 aacatatcaa acatgaagcc acaatacatc cgaacaatta ttcaggctgt taatgaaata
    13561 ctaaggccgg ttgacaaact tgattttata tttgatgaag cataactttg acttacaaac
    13621 attgatacac tataaaaaat aaataaaatg tctcattcta acaaagaaaa taaaggtatt
    13681 tacaagcaaa tggtattcag aaaaggtttg ttccaaatta aaaatctgaa ggataataag
    13741 gttttcctga aaaccagttc cgacttggac cgcgccttca attcggatag ctttcagttg
    13801 cgtcttggca gtcacaggaa cactgctttg caacaagact ggaactcatt aggtcaggat
    13861 aactttgagt tctctatact tgatgagctg aatacggatg aaaatcacac agataaggac
    13921 ataaaaaagg aactgaaaca actacatgaa ttgtacatag cggaatgtaa caaggaaggt
    13981 attgtgttgt attgacgtga tttttatgga gtcttaccaa gtatttttta cttgaactca
    14041 gataaccaat tcagttaggc gaataatttt tttcgccact tgaggtatcc gtcgatgctc
    14101 atgaatacat aaaaagaata gagtgcggca aagtaatatc cacctctgga gaaaaacagg
    14161 tatgccatca cagcgtctat tatcatccag tatatccaat tttcaatttt cttgcgaacc
    14221 agcaggaatg tgttgaacac agaaaacacc gttgttaaag aatcaatata ggtggaggct
    14281 gcggatgtgt attttgcaaa tatgtaacca aatatgtatg ccagacaaaa tcccggaact
    14341 ataacaagta gatgttgatt tatactcatg cgggtaatgg gcaatacatc ccgcttattt
    14401 ttgtctcgct gccaatggta cataccaacg aatgctatgc cgatatagaa tatttgtagt
    14461 atgacgtcaa cataaagcct gaaataaaca aaggaagcgt aagcccagaa caagctactg
    14521 ataacagacc accaccagct gatgtattgc tcacgtgctg ctgaaaaaat ataaataacc
    14581 gcaaaaaaag aagcaataat ttctgcgtaa tacaatgttg ccggttgttc ttgttaaaaa
    14641 aaacaagccc gcgaacggtc attcacgggc attgtgctgt attttcttta tgcttatttc
    14701 actttagcct tataacctac tgtgtagata aatggaacac tcttgtcacc tacggtgtta
    14761 agcatcacgt taatactgtc atcaacgtct tcgcctttgc tcttcggtgt tgtgtctagt
    14821 acggctttga tggtaccctt accacccggc tttattacac cctttgtcca gtcgagtgtc
    14881 atacattcgc aggcgctgca caggtctatc tcgacgtcct gatcggagac atttgtaaac
    14941 tggtaagtga atgatcgctt gtctttataa ggaatgactc cgtaattgtg tgctgtagga
    15001 tcgaacctaa gcaaagacgg acctgaataa ctctttgttg gtttgagttt tggttccttt
    15061 tcggaagcag ctgttgtgcc tttcgagtca gactttggtt cctctttagg tgctacctta
    15121 ggcatatcac cagtcttatt gatgataaca ccttcttctt ttgatccttt cagcctcttc
    15181 ttttctatct tgatagaagt agggccagat acaattttaa attctgttct tctgttgaaa
    15241 cggtgttcgt catctgtaca ttctatgtca tttacacatt tgttcagaat ctttgtttca
    15301 ccataaccta cggcttgtat tttatcacct gctattccct ttttcacaag ccagtttttg
    15361 gctgaatttg cacgacgttg agacagcttc atattgtaat catcatcacc tcgggcatct
    15421 gtgtgagatg aaagttcgat gaccatatca ggatattgtt tcatcagatc gtagataagt
    15481 gttaggtcgc cttcagctga tggcaagatt ttgtcatcgt tgtagtcata gtaaatgtta
    15541 ttgagtcgta ttgcctcttc cgtactgtat atttcgtatt ccggttcttt tggtttgagg
    15601 tttactttgt atttaaatgc ttcatccttt gcgagagcta ccgtgttaat gcctttaatc
    15661 gtatcaggat aatacccatc tttgttgatg atcatggcgt aagatttttc gagcaccaat
    15721 ggaaaatcaa ccgtatttgt ctttccggaa tttttagatt cggtcacacc catcttgtct
    15781 ccggtcatct ctatcaattg tgcattgtac tgctgtacca ccttaccact gctcgcatcg
    15841 aacaccagcg tattgacggt agccttgaat ttttcaatat tgaaggaata aatatcgtcg
    15901 cagcaagtct tactcttgag cgatctgccg ccaggtctgt ttgacaccag gaaaccattg
    15961 tagccagcat catcgatggt aaaataccaa tcatcaacac tggtattgta gccttttccc
    16021 atattgaccg gagccgtcca accggtacct gtccatgtac tgtagaaaat atcgagtccg
    16081 cccagaccgg gataactatc agaactaaaa tacagctttc catccttgta gaaaggtgta
    16141 aattcgtcac ctattgagtt aattgctgtt ccaaggttga caggatcggt ataaacatca
    16201 tctgccttat atgtagcata gtaaatgtca taaccaccat ggcctcctgc catattggaa
    16261 gagaaaaaca aaacctcctt tccgaacagc tcgcctgtca ttgggtgtcg tgcctggaaa
    16321 tcaccattta ctccggctac ttccttagga gccccccaac catcaggacc gagtgtcgca
    16381 acatatattt tgctttctga aagaacatta cctttcaaca ccgctctgtt aaaatacatt
    16441 cttttgccat ctcttgaaaa ggtagcattt cctgtggaat aaccttcgcg gttaatctga
    16501 tcattcatta actcaccggg agcccatttg tcaccgtctt ttttcgaact gtatattttc
    16561 agaaaataat ctgtattctt tccatctaaa gctaccgcag aatcactatt gaatgaagca
    16621 tagaataact gtgtgccgtc gggcgtgata tacggagagg cttcagtagt tgccttgttg
    16681 atggctttac cgagatttgt tactgtcagt cttgaattct gttccatctt tggtgccatc
    16741 tgacaacctt tgatttctat ttcagccatt tttttcaaca ccggatcggt cgtgctgttg
    16801 gcaaattcag aaaactgtag gatggcatct tcataattct cattcatctt caaagccttg
    16861 gcataattga acctgagctg gctatattcg cctttcttat ccttattgag tactttgctg
    16921 taaaaattgg ctgccttatt atagtctctg agtataaagt tcaaatcggc aatcttagcc
    16981 tgggtaggca ggtctttctt gttgtcatcc aggtattttg tatagtattc aagtgaatta
    17041 taataatcca tcgaatcgta tcttgactct gcaattgata ggttggttct ggcagttgac
    17101 ctattgagtg gctgagccag tgtagtactg agtcctgcaa tcagaaaaca aaaaaacaat
    17161 atatatctgt tcattgcgat gaataaattt taaagaaaat aaattagaat ctcgggcaga
    17221 agataaccgt gttaactttt ggttttttat aaatcattcc tgtgtacatg gcagcaattt
    17281 cataacctcc gttgaacttg cctgacacac ctgaagtgtt ggcatcccat gaaaatccga
    17341 ctttcagatt atcatagtcc attccgatga gtatttcggc ggcatcacgc agcctgtatc
    17401 caagcccgaa attgagttgt accttggcct tggtattctt cagtttgtaa cctgcaagcc
    17461 cttgtaatac catttcattc tgacctgcaa tggtctggta aaggaaagaa gggctaatag
    17521 accataccgg attgagattg acattaaatg tgccatgccc tacgtattta tgtgacagtc
    17581 tgttttgcga gcccttgtca cctgaaaaag catattccgg tttgttgata tgtcccattg
    17641 cgaaaccaac gatgaaatcc atctgttggt tgagttttga tttgagtgta agaccggcat
    17701 tgatatcaag atagcctttt ttatcgccct gccccagatt gcggtcagca ctttgtattg
    17761 gaatgggtga ttcgagctcg tcaatgaatt ttattttatc actctgcagg tcaacattct
    17821 gactttggcg accaccttgc aacccaatgg taagtacgga tgttgacttt ttgtttaacc
    17881 ctaggtgata tgctaccgaa cccatgaatg cagtacgtcc aagcttggct gtaccggact
    17941 tgtcggctgt aatagcgact cctacaccta tccagtcatt ctttctgaat cctgtgatga
    18001 taggggcatc tacaaagaaa gatggtgttg agtaagccgg tatgatgctt gcccactgat
    18061 tgcggtaaat accgcctaca cgatacgacc ccaaatagtt gccggtaagt gccggattga
    18121 gtgtcatggg agacatattg tattgggtaa aatgtatgtc ctgagccata agaatgttcc
    18181 caacaaacag gcagataagt attgtaccaa gcttcaaaaa acgcatagtg ctgtatttta
    18241 ttaataatag aggcgaaata taagctttat tccaaaagca aagatagttt ttcaatgtta
    18301 aatttgcttt ttaattgtgc tttttttgtc ttgagttgat gagtgactca atatttttaa
    18361 cgataagtac catattctgc tgtttcatac agtgaaaatg ttttttcgga caatgttcgt
    18421 aaccgatttt actgcatggt ctgcaagaca agccatacac ctcatgattg tggtacactg
    18481 catccgtgtt gtccttgtac aagggataca ttccaaattc cggaatcgtg tttccccaaa
    18541 tgactaccat cggtgtaccc atagcagcac cggcgtgcat gagaccggta tcgggtgtta
    18601 caagacaaag tgcacgtttg agcacagcta ccgattcctg taatgtcaat tcacctgcag
    18661 cattgacaat gacagaggca ggcattgaat ttgctatctc ctctccgaat tgtttgtcac
    18721 cggggcctcc tagcaataca acaggtagcc caagccggtt tatgatttga atgtaaagat
    18781 ctatggtagg cagcttagtc tttcgggctg cacctatagc cattgccacg tagctggcgc
    18841 catcaggtat gccttttaga ttcaaaaacc tgtctgcatc agtaatattc tcatcgctta
    18901 tgaagaaatc gggtcctttg ccatcgtatt ttaaaccaac tctttctaat gcttcaaaat
    18961 aacgatccac aatatgcttt ttgggcaaaa tgttaatttt caatgccgtc atgaaccatt
    19021 tccttacatt cagcttgtca aaatctgtgg aaggtagctt tagtgagtag ataatctgct
    19081 tgctgcgtag atttttatgc aggtcaaata tgtggtcaaa ttgttcttcc cttaaacgac
    19141 gttccgcttc ttcatcccat cgttcgtagt agtggatctg gtcaatatac gggttacctg
    19201 ccaacacgtt agcaaatgat tttttcacca gaaaatgaat ctcggcacca agctgttgct
    19261 taatgcatcg tactaccggt gtggtcagta tgatgtctcc aatagaactt aatctgataa
    19321 taagaacctt cataaaacga atcagctctc taaggtatgg attctcagag agctgaagta
    19381 ctattattcg ttattttatt gcttaattat cttttctgtc tgataattat ttcctgacat
    19441 gattctcata aaatatgctc ctgcaggcag atcactgacg tttatatgct gcgtgacacc
    19501 tttacctaat ggattgagct cttccgaccg tacggtattg ccggttgaat tcactatttg
    19561 tactacacca ttgcccatat cgttgacagc cttaatgact acactattat tcaccagatt
    19621 cggaaacact gaccagttgg caatcttctc tgagggctcg tgcgtggcta cattgatggt
    19681 gcgtgtaccg ttgccaaacc agtaaccatt ggtagcatct gtaaacagct gagtacttga
    19741 cccgccatag ctaattttat ctgaggcaag ttgaaaagcg tgtatttcgt tggtattgac
    19801 tgtacctatg atgtagttat ccagtcttga atagttagga acaaacaagt ctgattggtt
    19861 agaaatggca tcctctttac ctgtctctat attcccaccc acaagataca ccgtgttttc
    19921 gatttcatca atatagtcaa atgctactat gtacggcgaa ttttttgaga aaacaggatt
    19981 accaatactc gtattttcag gcaatccact gtatagtttg ctgatatttc cgtcaccgaa
    20041 tgtattcttt ttcttgtcaa atacacggat aaacccaatg tcccaatagt tgatggtact
    20101 acctgcctgg tttttgattt cgttgtaggc gtcatacatc acataattgc cactgtagtc
    20161 ccattcgagt gcatcggcgt acaaaacatt gccggtcacc gaacccgaat agctaggatt
    20221 ataaagtgta aatgtctgcc cttgctgcag gttgaagtca tagacggtta ttttgttttc
    20281 tacagcattc gaaaccattg ctattcggct gccatctttt gacaccgcca cattgcccca
    20341 aaccgggctt gtgcttacct tggtctccgt tacttgacca ttggaataat taatctgtat
    20401 ggcatggata ttttcatctt cacctacata aaatatagca ctgccatcat ccgtaacaga
    20461 aaatctactc ttcggagttg tacccgaaat tacatcgatt gttgcaaagc cattgccatg
    20521 agcaagataa acctggttag ggatggcatc ggttgctacg ataaaatccg agcccggatt
    20581 cggattgagg ttcttctggt atttatcgcc actcgaactg ccacctgtac ctatgcctac
    20641 ctgggcgaag gcattctcag cagccgttac ctcagctgaa gagttgccat aaagatcctt
    20701 ggcagcctga accactgctg cacgagcgtc aatgaaaaca gatgatttga caagatattg
    20761 ggtaagtgct ctgtaatata ctttttcggc cttttcctta cctacattcg gactggtagc
    20821 aaataggtag aaagcatgat tggtaatacc actgttgatg tgtacaccac cgttgtcttc
    20881 tccaccatta tatttttcat tcatgtgctt tggctgccat ccgttgtcat ttaggctgtt
    20941 acctccattg tgcgggtccg acatacttcg catgatgccg gtagggaagt atgatttctt
    21001 ggcgacggtc tcacctatat tccagttgtc acggtctatc attacaccaa atacgtcagc
    21061 aaaagattca ttgagtgcac ccgattcatt ttgatataca aggttggcgc ttttctctac
    21121 gacgccgtgt gtcatttcgt ggccggctac gtcaagccct ccggcgagtg gcgtaaatcc
    21181 gatctttccg ttgccgtaaa acatcgcctg gccgttccag aaagcattat caagacctcc
    21241 accatcactg tcagctatat ttatgagcga caatatattt cctccactac cgtctattga
    21301 atttctgtta tgtgtggcaa gaaagtaatc atagcttttg gctccgttgt aatgtgccga
    21361 aacagatgat ttatccgtcc atgtattagt agttgaagtc acatctgcta ccgtaaaccc
    21421 cgagttctca gggcttttat ttagaccgtc aagtgttgct atcacaccca cagcatcagt
    21481 tggcagattt gattgcgcag gtttgaacat cggtttggtg atgtctataa gataatactt
    21541 gttaccagcc tggtaagtat tgagtaactg tggtttgttg ttgaggtcta tggcgttggc
    21601 tgttgccgga ccatccagtg gattcatgac tgctgcatgg cattcaccac tatggtgcat
    21661 ctgattatgc agtccacaga tggtattgta tgagtgaatg atattgcctg tcaaggcatc
    21721 tacaaaatac tcatatcggt gggcaacatt cgggtatatc tctatatgcc aggctaatct
    21781 ttcggcatgt gcgtcattgt tcttatgata aatgaccaac ttcgacttca cctgtacatg
    21841 atcttttacc agttttagtt cctgatctgt taggttttta agtgtagtaa gccttgatac
    21901 atcattcttc acattctgta tggcctgcac ttcagacaca gccggtgtaa ggcttgtaag
    21961 atccggcgta gggaaatacc tgccattcat cagataaggg ccatctgcat ccgtatgtac
    22021 ttttacttca gctccgaata cctcaatacc acccaacatc tgcgtataag taatatgtcg
    22081 tatgtgtagt tcatccgttt catcttctga taatgcaaat tcggtcattg gttcttttat
    22141 ctgcatttgt ctgcccataa actgtaaata tgtcattgct tgctggcgtg catcaacggg
    22201 caatacaacc gaacccggaa gtctgccctc tacccaaaca gggagaccat cttctgaata
    22261 catttttacc tttgggccat catgatattt ggcaaataca ggaagtccgc ccatcgaacg
    22321 gtactcaggt tcagactctg taaattgagc cttggcaccc ggcaccatag ctttgtaagc
    22381 tttgaattcg cccctgttac tttcatcaac tttgagaagt gggttgcggt tcacctcttt
    22441 tttaacgctt tgtgcgtaaa tagccgatac gctaaatacc agcaacatta tccatgctat
    22501 ctttttcatt tttctgaatt tttatttagt aattctttgg catcatgtac catacttttg
    22561 acggcagggt tcaccaaagt atcattattg gcccacacaa atttgtattg aatatctttt
    22621 tctttaaata tgataaaact cgaaaaatta cccggcttct gatattcatc cttcatccag
    22681 ctttgcttga atgctgtctt gaaaaatttc cttacttcag atttcttttt aatacgtatt
    22741 tcttcggttt tcttgtttgc agtggagttt ttgaacacct ggcccgattc gcagaaaata
    22801 tattcactgc caaagccgga tacaccaccc tggctgccaa ttattagttg tttttcaggc
    22861 aaattttctg ccgtgaaatg cgatgatttg cacgaaaaga ttgctgataa cagcatcaaa
    22921 acagataaaa tcttcagtct tctcaatttt actggattaa gaatttcatt aattggctgc
    22981 aatttaatca ttccaaccat tgctttaggt atattttgaa aaaaattatg cgataccagt
    23041 tctctataca gcctatttaa caattgttaa ataataaaat gctgttcgca cccagacggc
    23101 ataccaacat tgaacccaga ttgtatgaat ccctccggaa tgattcattt tctcacacaa
    23161 ccatcaacat atcaactatg tatggagcga ttggcagtag ctgcaagtgc tgcttctttg
    23221 atggcttcgg tatatgttgg gtgcgggtga caaatcctgg ctgcatcttc ggcacttgcc
    23281 ctaaactcca tcagcgttac accttccata atcatatcag cggctctggc acccactatg
    23341 tgtatgccca gaatctcgtc ggtggtagca tcggcgagta ttttcaccat cccgtccaca
    23401 tccatgctcg cccttgccct acccagtgcc ttgtatggaa acttgcctgt cttgtaggcc
    23461 atgccggctg ctttgagctg tgcttcagtc tgccctacag ccgctacttc tggccatgta
    23521 taaaccactc ccggtaccag gttgtagttg aggtggggtt tttggccggc tatagtttct
    23581 gctacaaaga caccgtcttc ttctgctttg tgtgccaaca tagggccttc tattacatca
    23641 cctatggcgt atatgccggg tacggctgtt tctaggtgat gattgactgg tatgcgacct
    23701 ctttcgtccg ttgtaagccc tgcattttcc agagccaatt ggtcagtata aggccttctg
    23761 ccaatagcta ccagacagta atcggcatgg acggaaaagg tttcgccttt tactgtattt
    23821 tctacctgta cttccacact actctcactt gcatgtacag ctgtcacttt atgcttcaga
    23881 tggaacttca tcctgagctt tttaagtgag cgtgtcagtt cttttgtaca atcaacatcc
    23941 attgtaggga tgatgctatc cgcaaactca actacttcta tttccgtacc gagtctggca
    24001 aaaacgctac cgagttcgag gccgatgaca cctccgccta ttatacacat acgcgcaggg
    24061 atgtctttga tctgcaatgc ctcagtactc gtgatgacac gttttttatc ataattgaat
    24121 gatgccggca cagtcggttt tgagcctgtt gctataataa tttttggtgc cgacagctcg
    24181 gtagtctgcc catcggtggc ttttacggtt accgtgtttt tatctttgaa gctaccaaca
    24241 ccatagtgca catctatctt gttctttttc atcaaaaact cgatcccttt ggtattttcg
    24301 agtactacct gtgccttgcg tgctaccatc ttgtttaagt cggcggtcaa gtccttcata
    24361 tatacgccat gagcctcaaa acccgtcttt gcattgtgat aatgctccga cgaatcgagc
    24421 aatgccttag atggtatgca gcctacattg aggcaagtac cacccaggct tggatatttt
    24481 tcaattagtg cggttttgaa acccaattgg gcacatctga tggctgctac atagccaccc
    24541 ggccccgaac ctattactac tacatcgtaa ttcatcttag gctaagtttt tatttcctgc
    24601 ttataatcag ttattctttc ttgtcgccct gctcaggctt atcacgcgga ggtcggttgc
    24661 ccggctttcc gtgaaacttc cttccctgcc tgtgttttgg gttattcggc ctgttagggt
    24721 tctggttact tcctctgttt gcatgatcat tacctgatac agtgccggtg tcgtttgtat
    24781 tgtcctgcag ggtattatcc tgtcttgtat tttcttgtct tgtattaacc cttggtgtgt
    24841 cttgccttct ttgttcagga gcatttgtag tacttgtagg tttgtctttt ggagtgctct
    24901 gctctctgct gtcacgcggc tttttcttct tctttttctt ccttttctca gccggcaact
    24961 ctacaaatcc ggttacgtct tcgtagtctt tctctacctc atcttcgttc actattgtgg
    25021 ctatctgtac ctcgtctaac gactccacaa gctttccggc tttcatgccg gctattattt
    25081 ccttcacctg aactactgcc agtgggtaaa attttcctct tccctgctcc ctttcattga
    25141 cataaaacat gatcttttta aacacatctg ttttgaccag gatggcaggc ccggcttgcg
    25201 tctgtatttt atcagccttc tccggaaaat cttccagggc atcaaggtaa acatcgagct
    25261 cataattgag gcagcatttg agccttccgc actgacccga cagcttactc tggttaatgg
    25321 caagattctg gtaccgggca gctgtagttg acaccgactt gaagtcagat aaccaggtcg
    25381 aacaacacaa ctcacgacca cacgaaccaa taccgcctat gcgtgcagct tcctgcctgg
    25441 ctcctatttg ccgcatctct accttcacct taaactcact tgcaaaatgc ttgattagct
    25501 ctctgaagtc aacccggccg tcagctgtgt acaagaaggt cgccttgcgg tggtctgcct
    25561 gcagttctac atcacacacc ttcatctcca gcttaagact tcggcttatc actcttgatt
    25621 tgaccattat ttcaggctcc ttttcacgtg cttcctgcat gcgctgcaga tcgcgttcat
    25681 tggccttacg gagcacatta ttgaacacgg tatccttttt aatatttttc ttcttgagct
    25741 gcaggttgac caggtcgccc gacaggctta tcctgcctac atcaaaccca ttgcccgtat
    25801 ccactaccac ccagtcgcct gatgtagcac gtgtgtgtgg cggattcgta taaaaactct
    25861 tcgttgatcc gttcttgaaa cttacctcta cgagttcgct ctcgtacgga tcgtgtatat
    25921 ctaaggctgc gagccagtca aaagtattaa gcctgttaca tccaccggag gcgcaacctc
    25981 cgcttttacc acatcccgaa gagccacaac tgcctccgga cgaacatcct gaacatgaca
    26041 tattttcatt ttatttatgc agtggttgtt tcaccacccc ggttttttta taaatatttt
    26101 taagctgtat agctgcatcg aacatgagca gccttatgtt ggcattccgc tccaatccga
    26161 acccacactc atccagcaat ctgcaaatct cgttaatttg ttctaaatct aataaatttt
    26221 ttatttttaa agcagtttct gccttatcat cactcagccg tagtgcctta cccactacct
    26281 ttaacaatac cagttcgtgc ataaagttca ggccgtatcg tacaaatttt ttctgctcct
    26341 cacggtttaa tgctgcatag ccatccgtcc agttattcat ttccacggca tttcccttaa
    26401 aacaaatacg cagccaactt ataaattgtt cgtcaagact acccacggta tgctctctca
    26461 gtcgtatggc atccgaataa ttacctcctg ccaacctcgt tatcaccgac gcttcgcctt
    26521 cttctacacc ttcagcaagc agtttatcgt atatgtcggc atcatgcagc agtggcaccc
    26581 tatacacttg gcagcgcgac aatatcgtac ttagtacccg atcctcattc tcggctatca
    26641 atacaaaaaa tgtatcagca ggcggctctt ctattatctt caacagccgg ttgccttcct
    26701 ttgccagata ctcagccatc catatcacct gcaccttcca cctaccctcg taggcttgca
    26761 tattcagctt ttttactatg ttcaggcact ccttcgcagt tatattccat tgtttcgact
    26821 cagcatcgtg tgctgctatc cagtcagcca tatccatgta tgcattctcc acaaacatct
    26881 tcctgaactg cggtaaaaat gtatccgata cagcctccgc cccaaatgcc ggcaccgtta
    26941 tgtgcaggtc agggtgtatc agcttcgatg tctttacgca ggcatgacat accccgcaag
    27001 catcatccgc agccggattc tcacaaaaaa catactgtgc aagggcaagc gccaacggca
    27061 gactgccatg acccgacttc cccgatagaa gaatagcatg tggcagacgc ccgtacttca
    27121 atgccgatag caggtcagct attacttttt tctgcccgat aatatcttta aacaacataa
    27181 ccctgcaaag ataatcattt ttccgcttaa gcctgcatac ttctgcatca cctgtgcccg
    27241 gcataccaac tgctacatac acatcaccca tcaattgtat tatttgggcg tgcccctgtc
    27301 gggtcaggct atccgcctta cttcgtgccg gcttctatcc cttacgcaga tggtaagtgg
    27361 t
//
LOCUS       DAOQQY010000009        25286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_585_length_25286_cov_5.896675, whole genome shotgun sequence.
ACCESSION   DAOQQY010000009 DAOQQY010000000
VERSION     DAOQQY010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 25286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25286
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_585_length_25286_cov_5.896675"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1530)
                     /locus_tag="PLE23_01185"
     CDS             complement(<1..1530)
                     /locus_tag="PLE23_01185"
                     /inference="COORDINATES: protein motif:HMM:NF015196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HMX81907.1"
                     /translation="MTSQLHKFSRHLLLSILMLAGITLFSQNWINDLVEEGKPLNFYE
                     IQKKANEYWTTHDPTEKGKGFKPYKRWENYWENRLMEDGTFPPAGITNRNFQKYLKSH
                     PLKQAEKALAGANWTSIGPNSTDGGYAGIGRINAIAFHPTNNNIIYLGSPGGGFWKTT
                     NGGVNWTCTTDNLSSLGVSAIVVKPSNPNEIFLATGDGDATDTWSFGVLKSTDGGNNW
                     AITGLDWSNSYNKVIREMIMDPNDENTMMLASNDGIYRTTDGAVTWAKVQSGNFYDIE
                     ANPNAATNTFYACTQTGFHKSTDNGATWTNVQNITGSNRIELAVAPSNSNVVYALTSL
                     SSNSGFKGLYRSDDSGSTYVLKSSSPNLLGWADDGSDSGGQGWYDLTLGVNPTDENTI
                     FTGGVTNWKSTDGGVTWALNSFWYGIPGVAEVHADKHHAVWRGSDFFQGNDGGIYRTT
                     DNGTTWVDISGDLVISQMYRLGVSQSDAKAIAGLQDNGTKLMQTNGTWSDVIGGDGME
                     CA"
     gene            complement(2037..6392)
                     /locus_tag="PLE23_01190"
     CDS             complement(2037..6392)
                     /locus_tag="PLE23_01190"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81908.1"
                     /translation="MPSILHLLTQNFKGTLLLLAALFVTQMVSAQCQLNCIQGLQVSL
                     NESCEALITPNDILKHPADCPGAKIVEILDKNNKVIPTNPVVTSAYIGQTLTVRVIHP
                     ASGNICWGSISIEDKLPPVFTNCKDYTLPCAMPLDQILDITAPTVADNCGGTVKVTRS
                     HTKVDLACGSLPFTAIVTVTTTAEDIYGNKSSCVQFINLTRSPITNVLFPKDYDDVTL
                     PALECSNANTDPSNTGYPTLDGFDLKDASCGYLVSYQDLVQNNCPGSYKILRTWTVMD
                     WCGGTPPMATGIQVIKVVDKEGPQVNCNFDVHKIKVLGSQAGPGYQGCASTIEFPAIQ
                     VSDNCSNYNQLTFATYTYDDKGILYSSPTNGATLTLPTGYYYVYYNVTDACGNVSFCY
                     IEYEVKDEVPPVVACESHFNVSLTDSVTLVNAETFDDGSYDGCSKVTFLARRMNNPKC
                     GFNNETAFDKTVPFYCCDINNGPVQVILRVFDAAGNYNDCMVEATVFDKIKPVLWCPK
                     DITVQCGSDYAPEAPKSYSKSVTPNTSISDVFAKSYDVPVNISGIPVDAVVTDLNVAV
                     DVSHSYIDQLTIKLKSPHGTTVTLFQGGNCGQLQADINGTFDDEGKTLDCTGGSPAIS
                     GVVKPNAGLLANFDGELLNSIDLGNGSFKDWVLTVTDNAPLGGGKINKVSLNFTYGTP
                     LALRPNVTDNTQKCGLTVNWTDLDQPNNCQDGFIRRQWSATDAFGNTSTCIQRIYFND
                     VTPLDVIFPKDLTIEDCVSFNDLANLGEPWHNGDCELVAVNKEDHIFNVVPDACYKIV
                     RCWTVINWCKYDKNASHTDLGIVLPGADLKFRDDGDGYFHYCQTIKIIDKVKPVLTCP
                     TNVVFSSYEPDCGPAFVQIDTLKWKDCTPDPVITYSVDYYKDGSINYVGNGANASGKY
                     PNGTHSVTFKVSDKCGNFSTCSFDVTVKDGKKPTPVCVPLAIPVMPSAGMISITPEMV
                     TKGSYDNCTPANKLVYSLSPNTFTCNELGDNLVTLTVTDEAGNTEFCTTIVNIQDGNN
                     ICTGGIISNVSGKIQNLNGTGIGQVSVAPGIGQASMATTDDQGNFTFKLKAGGNYSIY
                     PNKNINPLNGVSTQDIVQLSNHILGKKPLNSPYKWVAADANRDNKVTTGDIVAIRKLI
                     LHVVDEFPNNTSWRFIDGNYNFPATGNPLSDNIPEAYTMNNLPATGINASFVGVKVGD
                     VQGDAQTSNATGNSTEVRSDATLNLNVEDALIHAGEERIIDVKASEFKRINGYQFTLT
                     LDNDKLEYVNILPGKLSDLSEENFGTRFANIGMITTSWNSSSTSLVNDDVIFSVVVKA
                     QEDVQISKALSVTSALTKAEAYNQDEEVLNVGLQFTQNGKVVATEGFELYQNQPNPFS
                     ETTSIGFNLPEASNVSLTIYDMSGRIVRSYNNSFDKGYNTFELKTADLGATGVFYYRV
                     ETPTNSGTKKMVIVK"
     gene            complement(6750..10541)
                     /locus_tag="PLE23_01195"
     CDS             complement(6750..10541)
                     /locus_tag="PLE23_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81909.1"
                     /translation="MKNYPLKLADAFQHDLRNVPILVQWRRIFNPNQPLLFIFGILLS
                     ISANKLHAQCNLNCIQQVQVALNEGGIAQITPDLLLTQYPVPGCSTNISIEIKDKDGN
                     IYPNTLTCNELGKTFTVKVTDQSNGNYCSSSLSLIDQIKPQIIPVNLAIFCSDPTSPD
                     LLGYPTVTDNCGSIGSDKLSYTDAYIDMSCKTIINGQEITGQIFRTWSVTDTSGNFAT
                     ALQVISLKRAKIEDIVFPPNHDGVSAPKLDCADDPNDLKKTGVPTINGFSITNGAACE
                     LFVQHSDNVYNTCVNGSYSILRTWTVVDYCVDTFKIYVQVIRREDKDAPVIVLPADMT
                     IGTQSSSCTGIVDLPKTTATDNCSSFTITPKWQFGQGYGPFKNVPVGVHPVNYVATDG
                     CGNTSTVTMNVTVIDSSIPVAVCKKDLAIAMDDDGLVTANATLFDDGSFDNCGIQKIE
                     VRRGNDPYASSLTFTCNDISPTPVDVQLRVFDTNNLSNTCHVQVFISDPVKPTITCPS
                     DLTISCTQNAGDLNVTGQASATDNCSLSVINYTDVISLNSCNQGNVVRTWTATDKYNN
                     SKYCTQKITLVDQTPLVITWPADYATTQCGADVTPDITGWPSFENDDCEQLDVSYTDQ
                     FFNTAPPACFKVVRKWVVRNWCEFNPNIPGAGIWESTQVIEVSDGIAPVLSLPADITV
                     GTTSTTTCVANVVLPEATATDCSTSISIVNNSGYASKKNANASGTYPSGVHIVTFTAY
                     DGCGNATSGTMKITVVDDEPPVPACIKGLTIPLNSDGIAELTVGMIEIGTTDNCTPKS
                     AIKLELSPTKFDCNSVGDNNVTLVATDASGNSNFCVTTVNIQDNIGYCALVAKITGKV
                     SSVLGERMKGVTVLLNNEVEAITNDKGEYEFLDLQKKSNYFVAATKNTHILAGVSTQD
                     VLAMSKHILGVQSLDSPYKIIAADINNNGKVTTADLLALRRAILQIDDKFPNNKSWRF
                     VDANQKFNNPGNPFEQTIPEVITYDNLQSNKPNTDWVGIKVGDLNLSVTPNNIAGDPQ
                     VRNAAETLELTTDDMKLAAGYEYKIPVRAADFKSVQGFQYTLEYDAQQLEIQTILPGA
                     LKNMDESAMGMSKAGEGLIAISWVEPIQDKFGPDDVLFEIVLKAKTNVMLSNALSVGS
                     DFLAAEAYDKYDDVLGVSFKFNGVNETAKYELEQNMPNPFEFETSIPFVLQDASQVQV
                     EIFDMLGNTIKKIEGRFAQGRHLIKVTSDDLNNISGMYYYQLQVAGKKPITRKMILMH
                     E"
     gene            complement(10944..11921)
                     /locus_tag="PLE23_01200"
     CDS             complement(10944..11921)
                     /locus_tag="PLE23_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMX81910.1"
                     /translation="MKKLILYMLMLTAAVRLSAQDESANSQYLVNPYFVNPGAAGMNG
                     LHNFHGSYRTALTTLPNNPTTYYVSYDGLIGKRVGMGAMLMNDKVASLNKYKMQLSYA
                     YNWVASEGLKMGIGLNAEFHQLNLDKKAGDNSFQQPGDPAILAALDGAQYFDATAGIY
                     GVYKDKLSFGVSVPNLIRARLNKNTDAEESAFATAFQYFTANVGYRFDVKDYDFAVEP
                     SVMVRKSYGSSVITDLNVKTYFLKNTLMAGGTLRVIDRETLVGLLLGAKFGALSLYYN
                     YDVSFQQTQRYHSGAHEFSVGFEFAGKKKMDAQEEEKKMMEANPPMPAK"
     gene            complement(11956..15534)
                     /locus_tag="PLE23_01205"
     CDS             complement(11956..15534)
                     /locus_tag="PLE23_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HMX81911.1"
                     /translation="MNKFISMILFFFAISVAQTFAMAPTIKINPLDATINVDETITYS
                     VTADNFNHISVEFTVLFDSGKLDFVSFSDINPQLKEFNLNYIPPGTPNYPEGLKVSWL
                     DPNFQPQNLPNGSVLFKMTLKAKAPGLSQITVPCGAPYVCEAIDGSGNVFESFPPTTP
                     ANLLINGTVVLDKFTIGISDEQCDQGSEVCVKVKALKDFTNITLMQMRIEWDVTKLQF
                     LSVKNCNSSIALNCGSDINFNGTALFLSWFADPDKAPDGYTLPDGSTLFEICFKVLQP
                     LGSKVPVAFKDEQFSKNLFSDKNGDITDFNLLNGSVTTGPCGGNTNEKLTLTASQETA
                     STGSNVCVKITASNFDSLVTAKFGVSWDPAVVVLTSVQNCNPLLNIANCSLGQGPFAV
                     VGGFLQFNWQASAGQQATGVTLPANATLFEVCFTVLGGPGTSSNIVLGSQGTFTASAT
                     DAKGKTFDLAYNQGKVTVPGAGTVTLSVTDKDACPGKTVCIPITVKSFNAIESMEFAL
                     QWDPALLSNVVLQSCNPQLSLNCNCSGSSNFNCTPGLLVVTWFSQSGGSVTLPNDAVL
                     FELCFDVVGANGTTSPLNIIPNPNTGVIEFINGSGATVDVISVNQNISITNTACSQPC
                     NLTQTNTITNAACSGSNSGSILINVAGGTGNYSYTWSPPIPAGTGNNPVNLGAGIYNV
                     TVVDTGNPGCSAVFGPFEVLAGQGFSVEVNVTPANCEGTFGGAIQLVPSGSTGYSYNW
                     SPASPNSSTITNLPAGTWDVTITDAKGCTVVLKSIVVPKGQGNFNVVGEVKYPKCFGG
                     SDGSIITAVTPVGNYTYSWNTTPVQTTPNITNLKAGSYTVVVTDVASGCTRTETFVIQ
                     NPPAIVLTSVSTNASSVVSNDGSISVNPTGGAGNYTYVWSTIPPQTGKKAVNLAPGIY
                     FVTVSDGNGCTAVIQDTVGVDINPPKPGIELVNTQFDKYNGYGVSCPGTCNGILLANP
                     PAGAVEPFVWVWSSNAKGTNENLAFDLCEGTYGVTLTDATGQKYYAAKQVQLIAPSAL
                     TLDLVTSANPTPTAKAIVTGGVSPYEYKFNQNPFTGQNVSIVDGYEPLTVIVQDKNNC
                     TITKTVVGPLGRDCNEARLVITPNGDGINDYLILACANVFTLNKFTVFDRWGKVLLTR
                     SGYTNDWDGVDKQGNPLPEGGYFWALEYINQNGNLEVAKGSITIVR"
     gene            complement(15598..16380)
                     /locus_tag="PLE23_01210"
     CDS             complement(15598..16380)
                     /locus_tag="PLE23_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81912.1"
                     /translation="MKKLLTLFFYISVAVGSMSAQLYLTPDTSFAHAGDKAQVNIRVK
                     NFNKLTGLQFSLNWNPAVLKYDTVSNFNLPGLSADMIGADPDTLASGKIALLWTSFQP
                     LGNIMADGSTMLTFIFDVVGSKGDSTSVFVSDSPVFAEAYDSVGNEVPVIKGIGYVYV
                     KPPLYVNEKYSEQGKYRLYNPDPNPFLQNTKISWVMPQSGNASIIVTDLNGNQVYQKY
                     ARYYQGKNQLILNRKDFPAAGTYFVTLKSDNILLTRKLIRGN"
     gene            17213..20029
                     /gene="carB"
                     /locus_tag="PLE23_01215"
     CDS             17213..20029
                     /gene="carB"
                     /locus_tag="PLE23_01215"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMX81913.1"
                     /translation="MPKDSSIRSVLIIGSGPIIIGQACEFDYSGTQASRSLREEGIEV
                     TLINSNPATIMTDPVTTDNIYLMPLTVESIIEILEKHKIDAVLPTMGGQTALNLALKA
                     EEQGVWERYGVRMIGVDVDAIEKAENRELFRQLMIEIGLDVAPSYIANSYLEGKEAAQ
                     KIGFPLVIRPSFTLGGTGGGFVMKPEEFDEALKRGLNASPTHEVLVEKAVLGWKEFEL
                     ELLRDKNDNVVIICTVENLDPMGIHTGDSITVAPAMTLSDTAYQDMRDKAMLMMRSMG
                     NFAGGCNVQFAQNPETEQLIAIEINPRVSRSSALASKATGYPIAKIAAKLAIGYTLDE
                     LSNQITKTTSAYFEPSLDYVIVKMPRWNFDKFAGADHLLGLQMKSVGEVMAIGRSFTE
                     ALQKACQSQENNRSGLGADKKEWINTQDILERLEKASDDRIYRVKDALRLGIPEKTVH
                     KITLIDPWFLKQIKSLVKMEEQLMRYNLPEDIPAAFFRELKENGYSDAQIAWLLRVDE
                     SAVTRQRKKLNMLRTFKMVDTCAAEFEAKTPYFYSSFDTENESIPSKKKKIIVLGSGP
                     NRIGQGIEFDYCCVHGLLAIKEAGYEAIMINCNPETVSTDFDIADKLYFEPVYWEHIE
                     EIIDLEKPDGVIVQLGGQTALKLAENLFKKGINIVGTDFKSMDLAEDRGSFSDLLKVL
                     EIPYPKYGVADDVDEALKIAHEVGYPVLVRPSYVLGGQRMRIVINDDELERSVLSIFK
                     HLPGHNVLIDHFLDRAKEAEIDAICDGDDVHIMGIMEHIEPAGIHSGDSSAMLPTFSL
                     TQEEVEQMAFYAKKLAFALNIRGLINIQFAIKNGKVYVIEANPRASRTTPFIAKAYKV
                     PYLTIATHIMMGTKKLKDYDIKPQLDGYAIKVPVFSFEKFPGVDKTLGPEMKSTGEMI
                     YFIKDLKDPYFRELMNERSMYLYS"
     gene            20104..20847
                     /locus_tag="PLE23_01220"
     CDS             20104..20847
                     /locus_tag="PLE23_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMX81914.1"
                     /translation="MLRITFLIFSMCALFNYDTLMAQQLDGFVKMGGGVIQASNLNLQ
                     VKPFFISKTEISNKEYRTFLLWLEKNRSSDELAEATQALDVAQTKLLPKNYLKSSKYD
                     NYPIVGVSYRAAQLYCEYLNAIHPGLSGKFRLPDDREWLYASAISNDDMLHSEIAASK
                     DKSYNFKLTTEKTAPMPVNSLKANGFGIYNMVGNVAEWTNSPGRIKGGSWNDNVETLN
                     IKKPGKYNGQTAPSPFIGFRPVYFEEPVK"
     gene            complement(20813..21808)
                     /locus_tag="PLE23_01225"
     CDS             complement(20813..21808)
                     /locus_tag="PLE23_01225"
                     /inference="COORDINATES: protein motif:HMM:NF018378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HMX81915.1"
                     /translation="MPYKSTNYYLKTILLKLYGFISEWVPGIDDYPKIKPDTVKICDR
                     KRLNKLDLFYHTFSYFHHPNDLAQHLKMKDGIAQHRIESKLDHCSPFYPSYYGLVCFN
                     SYLKTGEELFLLDAKAQLDTLLKLGTETEDHFILYYNIPLPKFLLKGRWYAGITQATM
                     LSLALRRNQQNPDERLELVIHKLVKSMMVPGAEGGILINTPEGLPWIEEYPGAKPSYV
                     LNGYIFCIISLLEYEVLYPNIQISHFIDRLLDSLVRSFPYYQRGKYLKYSRLYPTLSN
                     IEYQGLYIGQFKHLYLLTGLEIFSQLAELYNSRISWVAFNEFFQITSPALQNKPA"
     gene            21940..22782
                     /locus_tag="PLE23_01230"
     CDS             21940..22782
                     /locus_tag="PLE23_01230"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX81916.1"
                     /translation="MEEKKIIIRPQNAFRFRTAELLEYREMIYFFAWRDTKIRYRHVL
                     LGIIWAIVQPLFMALVFTGILHTGLKVPTGDTPVFLYFLSGIIVWNFFSQSVIFSTTS
                     MLANAHIIRKVYFPRLAVPLAAIFTKLIDFVIDLIFFTVVALVYKFIFNHNINISGVW
                     LALLLAFPVLAIFTTSVSIFLSGLNVRFRDVRNFIPYFIQIMFFLTPVIYTTRAGNYP
                     LLKTILELNPLNFCIEIFRSNITAETFQLITIPWATVVLLIFSYLAAIYTFRKMEDNF
                     ADMI"
     gene            22779..23519
                     /locus_tag="PLE23_01235"
     CDS             22779..23519
                     /locus_tag="PLE23_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX81917.1"
                     /translation="MKTAIDVKEIWKEYVKTESSGANLRESLLRIPRQLFKTRQEKFY
                     ALQNVSFSLEKGESIALVGKNGAGKSTMLKILARITPPTRGECIIYGKIASLLEVGTG
                     FHPELTGRENIFLNGSILGMKKNDINARFDEIVEFSGVASFIDTQLKKYSSGMQMRLA
                     FSVAAFLNADILLIDEVFGVGDTEFQQKSLEKLRQISQDKGKSVIFVSHDLDILSKLT
                     TRGILLQDGCITADTDIHSCISKYRDSF"
     gene            complement(23579..>25286)
                     /locus_tag="PLE23_01240"
     CDS             complement(23579..>25286)
                     /locus_tag="PLE23_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81918.1"
                     /translation="FPFPTADPAKRASLISFLKDTIPSGYFVVFFTVQDAKNSYKPED
                     WAADSITLGTNIFQLLEAQGAKSIRNTANNAVPYSFGYQKDVKPLGEAIGKDIDDVAS
                     LNFGLEGSWDRGSVTTPLAGPAKSWTKLTADIEENTADGDYSDLAVYGLDKSKQNKQL
                     LLDLKSAKSADLSSIDASQYPYLQVVVNLKDTLFKTAPYVRYIRLYYSGVTELALNSA
                     NADYSFHKDTIDQGDILKLNMPVQNISGVGSDSVLVKYVLTAADLSQQVELKKQPAIG
                     AGEKMTFPFQRNTIDLKGDYKLDIEINPGPEQLETYYFNNQASLRFTVLPDRRNPLLN
                     VLFDGIHILDGDIVSPKPEITIELKDENKYISLTDTSSFKLFLRYPGKSVFDAIPISS
                     SEVSFIPAKGTGKSNIAKAIYKPAELKDGEYELLVQAKDASNNASGQSDFKIRFRVIN
                     QKSVSGFLPYPNPFSTAMGFVYTLTGDRTPEHVRIRIYSVSGSLVKEITEQELGILRV
                     GTHVTDYKWDGTDMYGNKLANGVYLYQVVIKDDAGKQWDSYTSKADDFLQHGFGKLVI
                     LR"
BASE COUNT         7286 a         5348 c         5165 g         7487 t
ORIGIN      
        1 ggcacattcc ataccgtcac ccccaataac atcactccaa gtaccattgg tttgcatgag
       61 tttggtaccg ttgtcctgca ggccggctat tgcctttgca tctgactgag atacgccgag
      121 ccgatacatc tgggagataa ctagatcacc tgaaatatct acccaagtag taccattatc
      181 ggttgttctg tatatgccac catcattccc ctggaagaaa tcagagcctc gccacacagc
      241 atgatgctta tcggcatgta cttcggctac acccggtatg ccgtaccaga aggaattcag
      301 cgcccatgta acgccgccat cggttgattt ccagttcgtg acaccaccgg taaatattgt
      361 attttcgtca gttggattta cacccaaggt gaggtcgtac caaccttgcc ctcctgagtc
      421 tgacccatcg tctgcccagc caagaaggtt gggagagctt gactttaaca cataagtaga
      481 tccgctgtca tccgatctgt acagaccttt gaacccactg tttgaagata aagaagtaag
      541 ggcataaacc acattgctgt tagaaggtgc cacagccagt tctatcctgt tgctgcctgt
      601 aatgttctgt acattggtcc aggtggcgcc attatcagtt gatttgtgaa atccggtctg
      661 cgtacaggca tagaaagtat ttgtagcagc attaggattt gcctcaatat cgtaaaaatt
      721 ccccgactga acttttgccc atgtaaccgc tccgtcagta gtgcggtaga taccgtcatt
      781 tgatgccagc atcatggtat tttcgtcatt cgggtccatg atcatttcac ggatcacttt
      841 gttgtaggaa ttcgaccagt caagacctgt tattgcccaa ttatttcctc catctgttga
      901 cttcaataca ccaaaagacc aggtatcggt agcatcacca tcgcctgttg ccaggaatat
      961 ctcattagga ttggaaggtt ttactacaat ggctgaaaca ccaagagaag ataaattgtc
     1021 tgttgtacat gtccagttga caccaccatt ggttgttttc cagaatccac cgccgggtga
     1081 ccctagatat attatattat tattggtggg atgaaatgca atagcattaa ttcttccgat
     1141 acctgcatat cccccatcgg tactatttgg gccaatagag gtccagttag cgcctgccaa
     1201 agccttttca gcttgtttga gcgggtgaga tttgaggtat ttctgaaaat tacggtttgt
     1261 gataccagcc ggtgggaatg ttccgtcttc catcaatcga ttttcccaat agttttccca
     1321 tcttttgtaa ggtttaaagc ctttaccttt ttcagttggg tcatgagttg tccagtattc
     1381 attggccttt ttctgaattt cgtaaaaatt taaaggctta ccttcttcga ccaggtcatt
     1441 aatccagttt tggctgaata atgttatgcc tgccagcatc aaaatgctca gtaagaggtg
     1501 gcgactaaat ttatgtaatt gtgatgtcat gggttttggc tttttagttc agggcattaa
     1561 tttgttgtta ggtatttctg tttgaatatt gctaaaataa aaatagccct ccgtgttgtc
     1621 ggaaagctta tgaatacagg gaccgggaat caataaatgt ggtaaaacat aggctgggaa
     1681 cctatatttt gtgcaataaa aagactttct tttatcagag aaaagataaa gtacggtaca
     1741 acagccttga tagagcaacg tgtgcatttt accggataat aaaattaaac ttccttttag
     1801 aggaaattaa attaataaca tatcacggtt ttcgggaaga aaaaaaagat ggtgtaaagt
     1861 tatatataat ttatgatgaa aagcaagttg cagacgtaaa tttaggtaaa aaaaaagccc
     1921 cccaccacct aagtggagag ggaccaatcc caaaaaccgt caagtttaat ttttttttct
     1981 tatgggtaaa ttcagtatgt ctggcgcttt tttatttgta tatcccttac aaatgattac
     2041 ttaacaatca ccattttctt agtaccactg ttagttggag tttcaactct gtagtagaat
     2101 actccggtag caccaaggtc agcagttttt aattcaaagg tattgtaacc tttatcaaaa
     2161 ctgttgttgt aagatctcac tatacggcca gacatatcat aaattgtcaa agaaacattt
     2221 gatgcttcag gcagattaaa cccaatgctt gttgtttcgc taaaagggtt tggctgattc
     2281 tgatacagtt caaatccttc cgttgcaaca accttgccat tttgagtaaa ttgaagtcct
     2341 acgtttagta cttcttcatc ttgattataa gcttcagctt tagtcagtgc agatgttaca
     2401 gaaagcgcct tactgatttg tacatcttcc tgagctttta caactactga gaaaataaca
     2461 tcgtcattaa ccagtgaagt tgatgagctg ttccagcttg tagtaatcat acctatatta
     2521 gcaaatctcg taccgaaatt ttcttcagaa aggtctgaca atttacctgg aagaatattt
     2581 acatattcaa gtttatcatt atcaagagta agtgtaaact ggtagccgtt aattcttttg
     2641 aattcgctgg cttttacatc aattattctt tcttcacctg catgaatgag tgcatcttcc
     2701 acattgaggt taagtgttgc atccgaacgt acttctgtac tgttacctgt agcattgcta
     2761 gtctgagcat caccttgtac gtcacccacc tttacaccta caaaactggc attgataccg
     2821 gttgcaggca gattattcat tgtgtacgct tcaggtatgt tgtcagacaa tgggttgccg
     2881 gtagcaggga agttgtaatt accgtctata aatctccatg aagtattatt cgggaattcg
     2941 tccaccacgt gcagaatcag ttttctgata gcaacgatgt cacctgtagt aactttattg
     3001 tctctgttag catccgcagc tacccactta taagggctgt tcaaaggttt cttaccaaga
     3061 atgtggttag aaagttgaac gatatcttga gtagatacac cattgagtgg attgatgttt
     3121 ttatttggat aaatagagta gttaccaccg gctttcagtt taaaggtgaa gttaccctga
     3181 tcatccgttg ttgccataga agcctgacca atgccaggtg ctactgatac ctgaccgata
     3241 cccgtaccat ttaggttctg gattttaccg gacacattgg agatgatacc acctgtacag
     3301 atgttgttac catcctgaat attgacaatt gtagtacaga attcagtatt accagcttcg
     3361 tcagttactg tcaatgttac cagattgtct ccaagttcgt tacatgtgaa tgtattaggt
     3421 gatagtgaat agaccagctt gttggcaggt gtacagttgt catagctacc tttggttacc
     3481 atttcaggtg tgatggagat catacctgca cttggcatta ccggaatagc aagcggaaca
     3541 cataccggag taggtttctt accatcttta acagttacat caaatgaaca agtagagaaa
     3601 ttaccacatt tgtcagacac tttgaacgta acagagtgtg ttccgttagg gtattttcca
     3661 ctggcattag caccattacc tacgtagttt atagaaccat ccttgtagta atcaacgctg
     3721 taagtgataa ccgggtcagg agtacagtct ttccatttca aagtatcgat ctgtacaaaa
     3781 gctggaccgc agtcaggctc atagcttgag aaaactacat tagtagggca tgtaagcacc
     3841 ggctttactt tgtcaatgat tttgatggtc tgacaatagt ggaagtaacc atcaccgtca
     3901 tctctgaact taagatcagc accaggcaga acgattccaa gatcagtgtg tgatgcattc
     3961 ttgtcatatt tacaccagtt gattactgtc cagcatctta cgattttgta acaagcatca
     4021 ggtactacgt tgaatatgtg atcttccttg ttaactgcaa caagctcgca gtcaccgtta
     4081 tgccatggct caccaagatt ggcaaggtca ttgaagctaa cgcaatcttc tatggtaaga
     4141 tctttaggga agataacatc tagcggagta acatcattga agtagattct ttgaatacat
     4201 gttgaagtat tgccaaaagc atccgtagcc gaccattgtc ttctgatgaa accatcctga
     4261 cagttgttag gctggtcaag gtctgtccaa ttgacagtaa ggccacattt ctgtgtatta
     4321 tcagtaacat tcggtcttag agcaagcggt gtaccgtaag taaagttcag gcttaccttg
     4381 ttgatttttc cacctcctag aggagcatta tccgtaacag taagaaccca gtctttgaat
     4441 gaaccatttc cgaggtcaat agagttaagt agttcgccat cgaagttggc aagtaatcct
     4501 gcatttggtt ttaccacacc actgatagcc gggctaccgc ctgtacagtc tagtgtcttg
     4561 ccttcatcat caaatgtacc gttgatatct gcctgtagct gaccacaatt gccaccctgg
     4621 aacaaagtaa ccgtagtacc gtgcgggctc tttagcttaa tagtaagctg atcgatataa
     4681 ctgtgtgaca catcaactgc tacattaagg tcagtaacaa ctgcgtcaac aggaatacct
     4741 gaaatattaa caggaacatc gtaagattta gcaaaaacat cactaattga agtattaggt
     4801 gttacagact tagagtaact cttaggagct tcaggtgcat agtcgcttcc gcactgtact
     4861 gtaatgtcct taggacacca aagcacaggc ttgattttgt caaaaacggt agcttcaacc
     4921 atacagtcat tgtaattgcc cgctgcatca aaaactctca aaataacctg aaccggacca
     4981 ttattgatat cgcaacaata gaaaggtaca gtcttatcaa atgcagtttc gttgttaaat
     5041 ccgcatttag gattgttcat ccttcttgcc aggaatgtca ccttgctgca tccgtcataa
     5101 ctaccatcat caaatgtttc ggcatttaca agcgttacag agtcggtaag tgacacattg
     5161 aaatgcgact cacatgctac taccggtggc acttcatcct taacttcgta ctcaatatag
     5221 cagaatgata cattgccaca tgcatcggtc acattgtaat aaacatagta gtaacctgtt
     5281 ggcaaagtaa gtgttgcacc attggtaggt gaagagtaaa ggattccctt gtcatcataa
     5341 gtataagtag cgaaagtaag ctgattgtag tttgagcaat tatccgatac ctgaatggcc
     5401 ggaaattcaa ttgtagaagc gcaaccctga taaccaggac ctgcctgtga accaagtact
     5461 ttgattttgt gaacgtcaaa attgcaattc acctgagggc cttctttgtc aacaaccttg
     5521 ataacctgga taccagtagc cattggaggt gtacctccgc accagtccat cacagtccat
     5581 gttctaagaa tcttatagct gccagggcaa ttattctgaa caaggtcctg atatgaaaca
     5641 agataaccac aagatgcatc cttcaggtca aacccatcaa gcgtaggata acctgtattt
     5701 gaaggatcgg tgtttgcatt agagcactca agtgcaggca atgttacatc atcataatcc
     5761 tttgggaaaa gtacatttgt gataggactt cttgtaaggt tgatgaattg tacgcaagaa
     5821 gatttgttgc cataaatatc ttctgcagta gtagtaacag taacaatagc agtaaaaggc
     5881 agagatccgc atgcaaggtc aaccttggtg tgagatctgg taacctttac tgttcctccg
     5941 caattgtcag caactgtcgg agctgtaata tccaatatct gatcgagagg catagcacat
     6001 ggtaaggtgt aatctttaca gtttgtaaat accggaggta gtttgtcttc aatagaaata
     6061 cttccccagc agatgttgcc tgaagccggg tgaataaccc ttacagtcaa tgtctgacca
     6121 atataagcac tggtcactac cggattagta gggatcactt tgttgttttt atccaagatt
     6181 tcaacaattt ttgctcccgg gcaatcagcc ggatgcttaa ggatgtcgtt aggcgtaatc
     6241 aatgcttcac agctttcatt aagcgaaacc tgcaagccct gaatacaatt gagctggcac
     6301 tgagcagaca ccatctgggt cacgaacaga gccgccagta gcaacaaagt acctttgaag
     6361 ttttgtgtaa gtaaatggag aattgaaggc actgaccttc ggttgttttg atttacgatt
     6421 tgaaatcggt ttctcatggg aatacagttt ttgttaaaga attttttagg attttgatat
     6481 gtttctctat gtgtacagga aattaaaaaa catttttacg gtgttttggt cacggttttt
     6541 acggtcagta gtaacattca attcatagtc aaatttttta attcggcaat tttcgcaatt
     6601 gtaaatgttt cagatatagg atttattttt ttggtttctc gatgtttgtt tattgttgat
     6661 ttttgttaaa gttgttggtg cccttaagct ttattttaag ggctaccaac acccaaatcc
     6721 caaaaaccgt caataaaaat gacctgatgt tattcgtgca tgagtatcat cttgcgagtg
     6781 ataggttttt tgcctgccac ctgaagctga tagtaataca tacccgaaat gttgttcaga
     6841 tcatctgatg ttacttttat taaatgcctg ccctgtgcga accggccttc tattttcttt
     6901 atcgtgttgc caagcatgtc aaatatttct acctgcacct gcgaggcatc ttgtagcaca
     6961 aatggaatac ttgtttcgaa ctcgaacggg ttaggcatat tctgctccag ttcgtatttt
     7021 gcagtttcat tcactccatt gaatttgaat gacacaccaa gtacatcatc atatttatca
     7081 taagcctcag ctgcaaggaa atcagaacct accgatagtg cattgctaag cataacgttg
     7141 gttttggcct ttagaactat ttcgaacaaa acatcgtcag gaccaaattt gtcttgtata
     7201 ggttctaccc agcttatggc tatcaaacct tctcctgctt ttgacatacc catggcagat
     7261 tcatccatgt ttttcaaagc tccaggcaat atcgtttgta tttctaattg ctgagcgtcg
     7321 tactcaagtg tgtactgaaa tccctgaaca gacttaaaat cagcagctct gacaggaatt
     7381 ttgtattcgt agcctgcagc caacttcatg tcatcagtcg tcagttcaag ggtttctgca
     7441 gcatttctta cctgcgggtc accggcaata ttatttggtg ttaccgaaag attaaggtca
     7501 cctactttaa tacctaccca gtcagtatta ggtttgttgc tctgcagatt gtcatacgtt
     7561 ataacctccg gaatggtctg ttcgaaagga ttaccagggt tgttaaattt ctggttagca
     7621 tccacgaacc tccatgactt attgttcgga aacttgtcgt ctatctgcaa aattgctctt
     7681 cttagcgcca gcagatcggc cgttgttact tttccattgt tgttgatatc agctgctata
     7741 atcttataag gcgaatcaag cgactgaaca cccagtatgt gcttagacat tgccagtaca
     7801 tcctgtgtcg aaacaccggc cagaatatgt gtattcttag tagcagccac aaagtagttt
     7861 gactttttct gcaggtctaa gaattcatat tcgcctttat cattagttat tgcttctact
     7921 tcattgttga gcagaacggt tacacctttc attctttcac caagtacgct gcttactttg
     7981 cccgttatct ttgctactaa tgcacaatag ccgatattgt cctgaatatt caccgtggtg
     8041 acacagaaat tagaattgcc cgatgcatca gttgctacca aggtcacatt attgtcgcct
     8101 acactgttgc agtcaaactt ggttggtgac aattcaagtt taatggctga ctttggagtg
     8161 cagttatcag tagtacctat ttctatcatg cctactgtaa gctctgcaat accatcagag
     8221 ttaagcggga tggttaatcc cttgatacat gcaggtaccg gtggttcgtc gtccactaca
     8281 gttattttca ttgtacctga agtagcatta ccacatccat cataagctgt gaatgtcaca
     8341 atatgtacac ctgaaggata tgtgccgctt gcattggcat tcttttttga tgcatagcct
     8401 gaattgttga ctatgcttat gcttgtgcta cagtcggtag cagttgcttc aggcaatact
     8461 acattggcaa cgcaggtagt ggtagaagtg gtacctacgg taatatctgc cggcaagcta
     8521 agtaccggtg caataccatc acttacttct atcacttgag tgctctccca gataccggca
     8581 cccggaatgt tcggattgaa ctcacaccag ttgcgtacta cccattttct cactacttta
     8641 aagcaagccg gcggagctgt attgaaaaac tggtcggtgt aactcacatc cagttgctcg
     8701 caatcatcat tctcaaatga aggccagccg gtgatatccg gcgtaacatc tgcaccgcac
     8761 tgagtggttg cataatctgc aggccaggta attacgagtg gggtctggtc aacgagggtt
     8821 atcttttgtg tacaatattt cgaattgttg tatttgtcag tggctgtcca tgttcttaca
     8881 acattgccct gattgcaact atttaggcta ataacatcag tatagttaat aacagacagc
     8941 gaacaattat cggttgcaga agcctgcccg gtcacattca ggtcacccgc attctgagta
     9001 catgagatgg tcagatcaga agggcaagta atggttggct ttaccggatc agatataaaa
     9061 acctgtacat ggcaggtatt agacaaattg tttgtgtcaa atactctaag ctgcacatct
     9121 accggagtag gagaaatatc attgcaagta aatgtaagag aagaagcata aggatcgtta
     9181 ccgcgtctta cttctatttt ctggatacca caattatcaa aactaccgtc atcaaataat
     9241 gtagcattcg cagtcaccaa accatcatca tccatagcga tagcaaggtc tttcttacat
     9301 actgctacag gaatggagct atctataacc gtaacattca tcgttacggt agaagtattg
     9361 ccacatccgt ctgtggcaac ataattgacc ggatgcacac caacaggtac attcttgaaa
     9421 ggtccatagc cttgaccaaa ctgccactta ggcgtaatcg taaaactcga acagttatca
     9481 gtagccgtag tctttggtag atcaacaata cctgtacatg aactgctttg cgtaccaatg
     9541 gtcatgtcgg caggcaatac gatgacaggt gcatctttat cttcccttct gatgacctgt
     9601 acatatattt tgaaggtgtc aacacagtaa tcaactactg tccaggttcg gagtatagaa
     9661 taacttccgt tgacacaagt attataaaca ttgtcagaat gctgtacgaa caattcgcat
     9721 gcagcaccat tagtgattga aaatccgtta atggtaggaa caccggtttt tttcaaatca
     9781 ttcgggtcat cagcacaatc cagttttggc gcactgacac catcatggtt aggcggaaaa
     9841 acgatgtcct caattttagc tcttttcaag ctgattacct gcagagctgt ggcgaagtta
     9901 ccggaagtat ccgttactga ccaggtacga aatatttgac cggttatttc ctggccattg
     9961 atgattgttt tacatgacat atcaatatat gcatcggtat aacttagctt gtctgagcca
    10021 atgctaccgc aattgtcagt cacagtcggg tagcccaaca gatctggcga tgtggggtca
    10081 gaacaaaata tggctagatt gacgggaata atttgaggtt tgatctggtc tatgagacta
    10141 aggcttgatg aacaataatt accattagac tggtcagtaa ccttaacagt gaatgtttta
    10201 cccaactcat tgcacgtgag tgtatttggg taaatatttc cgtctttatc ctttatttct
    10261 attgaaatat tcgtactaca accaggtacc ggatattgcg tcagcagcaa atcaggtgtt
    10321 atctgagcaa tgcctccctc attgagcgct acctgcactt gctgaatgca gtttaagttg
    10381 cactgggcat gaagcttatt ggctgaaata ctcagcaata taccgaagat aaataatagt
    10441 ggttggttcg ggttaaatat ccttctccac tgcacgagga ttggcacatt tctcaagtcg
    10501 tgctggaaag catcggctag tttaagcgga taatttttca tgggacgaat tttggttttt
    10561 ggattatagt cttttcttac tggctaactt aacatgtgta ggttctggtt gcttctctct
    10621 aatgacaatt acattaaagc cgaaagtctc agatgttaca atgtgcagga atgtcaggat
    10681 acaacgaatg ttgtggtttg cagtagaaag cgagtcgtcc tgaaggaagg gaattaaata
    10741 ttccctgggt ttgttttacg gttttgggac agtctcacgt atgttcttaa tatacttggt
    10801 agtgttttcg gtttttactt gtgatatttt tattatgcaa aaagtatgct aatttttaat
    10861 atattgaaaa aaaatatgtt ttttgataaa aaattttggc agcttccttt ctttcatatc
    10921 cgggaagctg ccagaatatt ggattactta gccggcatag gcggattggc ttccatcatt
    10981 ttcttttctt cttcctgcgc atccattttt ttctttccgg caaattcgaa acctacagaa
    11041 aactcatgag cacccgaatg atagcgttgt gtctgttgga atgaaacatc atagttgtag
    11101 tacagactca gtgccccgaa tttagccccg agcaacagac ctaccaaggt ttcgcggtct
    11161 attactcgca atgtaccacc tgccattagg gtatttttca gaaaataggt ttttacattc
    11221 aaatcagtaa taactgaaga cccgtatgat tttcttacca ttacagatgg ctcgacagca
    11281 aagtcataat cttttacgtc aaaacggtag cctacattag ctgtaaaata ctggaaggca
    11341 gtagcaaaag ctgattcttc tgcatccgtg tttttgttaa gtcttgctct tatgagattc
    11401 ggtaccgaga caccaaagct tagtttgtct ttataaacac cgtaaatacc tgctgtagcg
    11461 tcaaaatact gtgcaccatc caatgctgca agaatagctg gatcaccagg ctgctgaaat
    11521 gagttgtcac ctgctttttt gtcaagatta agttggtgga attcggcatt aagaccgata
    11581 cccatcttca ggccttcact tgccacccaa ttgtaagcat aggacaactg cattttatac
    11641 ttatttaatg atgctacttt gtcattcatc aacattgcgc ccatacctac tctcttgcca
    11701 atgagcccat catacgacac gtagtatgtt gttgggttat tgggtaaggt agtaagtgct
    11761 gtgcggtatg atccatgaaa attatgcaga ccgttcatgc ctgcagcacc aggattgaca
    11821 aaatatggat tcacaaggta ttgcgaattg gctgattcgt cctgagcaga caatctgact
    11881 gcagcagtga gcatcaacat atatagaatt aactttttca ttcttaaggt tttttttgaa
    11941 atttaacgaa tcagtttagc ggactatggt aatacttccc ttagctactt cgagattgcc
    12001 attctggttg atatattcca aagcccagaa atagccacct tccggtagag ggttgccttg
    12061 tttgtcaaca ccgtcccagt cgttggtata tccacttcgg gtcagaagca ctttgcccca
    12121 tctgtcgaat actgtaaact tattcagcgt aaagacattg gcacaggcaa gtatcagata
    12181 atcgttgata ccatctccgt taggtgtaat cacgagtctt gcctcattgc agtcgcgacc
    12241 aagcgggcct acaactgttt tggtgatggt acaattattt ttatcttgca ctattaccgt
    12301 caatggttcg tagccatcta ctatagagac attctgtccg gtaaacggat tttgattaaa
    12361 tttatattcg taaggcgaaa cacctcctgt aacaatagcc ttagccgttg gtgttgggtt
    12421 tgcacttgtc actaaatcaa gcgttaatgc cgaaggtgct atgagctgta cctgtttggc
    12481 agcataatat ttctgcccgg tggcatccgt gagtgtaaca ccataagtac cttcacataa
    12541 gtcaaaggca agattctcat tggtaccctt cgcattactt gaccataccc aaacgaatgg
    12601 ttcaacagct ccggccggtg gattggcaag taggatacca ttgcaagtac ccggacagct
    12661 tacaccataa ccattatatt tatcaaattg tgtattcacg agctcaatgc ctggtttagg
    12721 aggatttatg tccaccccaa cagtatcctg aataactgca gtacaaccat taccatcgct
    12781 aacagtcaca aaatatatac ctggagctaa gttaacagcc tttttgcctg tctgtggtgg
    12841 gatggtactc cacacataag tataattgcc tgcaccacct gtagggttaa ccgatatcga
    12901 cccatcatta gaaaccaccg aagaagcatt tgtactgaca gaggtaagta ctatagcagg
    12961 aggattctgg ataacaaacg tttctgtcct cgtacatcct gacgctacat ctgtgactac
    13021 tacggtatat gatccggctt tcaggtttgt gatatttggt gttgtctgca caggtgtggt
    13081 attccatgag tatgtataat tgccgaccgg tgtaacggca gtgatgattg atccgtcgct
    13141 tcctccaaag catttaggat atttgacttc gcctacaaca ttgaagttcc cctggccttt
    13201 aggtaccaca atgctcttga gtaccactgt acagcctttg gcatcggtga tggttacatc
    13261 ccatgtacca gcaggtagat tcgtaatggt tgagctgtta ggtgaagcag ggctccagtt
    13321 ataactatat cctgtagaac ccgaaggaac cagctggata gcgccaccaa atgtaccttc
    13381 acaattagcc ggtgtaacgt ttacttctac gctaaaacct tggcctgcca atacttcgaa
    13441 cggaccaaac acagccgagc aaccaggatt gccggtatct actacagtaa cattatatat
    13501 gcctgcacca aggttaacag gattattgcc tgtacctgca ggtataggag gcgaccaagt
    13561 atagctataa ttacctgtac cacctgctac attgatcagg atcgaaccag agttactgcc
    13621 cgaacaggct gcattggtta ttgtattggt ttgcgtaaga ttgcaaggct gagagcaagc
    13681 tgtattagtt attgaaatat tctggttgac agagatgaca tctacagtcg caccgcttcc
    13741 attaataaat tcgataacac ctgtattagg gtttggaatg atatttagag gtgatgtagt
    13801 accattggcg cctaccacat caaaacacaa ttcgaacaat actgcatcat tcggcaaggt
    13861 caccgaacca cctgactgag agaaccatgt aacgaccaac aatccgggtg tacagttgaa
    13921 gtttgaagag ccactgcagt tacagttaag gctgagttgc gggttacaac tttgaagcac
    13981 aacattagac aacaaggcag ggtcccactg tagggcaaac tccattgatt ctatagcgtt
    14041 gaagcttttc acagtgattg gaatacatac cgttttgccc gggcaagcgt ccttatcggt
    14101 cacactcagt gttacggtac cggcacccgg cactgtcact ttgccctgat tgtaggccaa
    14161 atcaaatgtt tttcctttgg catccgttgc acttgctgta aatgttccct gcgagccaag
    14221 aacgatgttg ctgctggtac ccggccctcc gagtacagta aagcacactt cgaacaaagt
    14281 agcattcgca ggtaaggtaa cgcctgttgc ctgttggccg gcactagcct gccagttgaa
    14341 ctgcaagaac ccacccacaa cggcaaatgg cccctgaccg agactgcaat tagcaatatt
    14401 cagcaaagga ttacaattct gcacacttgt aagtactaca acagccggat cccagcttac
    14461 gccaaacttt gcagtaacaa ggctgtcaaa gttagatgct gttattttaa cacatacatt
    14521 cgagccggta gaagctgtct cctgtgatgc agtaagtgtc agtttttcgt ttgtattacc
    14581 tccgcacggg ccggtagtca ccgatccatt taataggtta aagtcggtaa tatcaccatt
    14641 tttatccgag aaaagattct tcgaaaactg ttcgtccttg aatgcgacag gcactttaga
    14701 gccaaggggc tgcaaaactt tgaagcatat ttcaaacagc gtagaaccgt caggcaatgt
    14761 atagccgtca ggagctttat cggggtcagc aaaccaactc agaaacagag ccgtgccgtt
    14821 aaagtttata tctgagccac aattaagggc aatagatgag ttacaatttt ttacactcaa
    14881 aaactgaagt tttgtcacat cccattcgat cctcatttgc attagggtga tgttggtaaa
    14941 atctttcaaa gctttcactt ttacacatac ctctgatcct tgatcacatt gttcatctga
    15001 gatgcctata gtaaatttgt ctaatacgac tgtaccatta atcaacagat ttgcaggtgt
    15061 ggtgggtgga aatgattcga aaacattgcc gcttccgtct atggcctcac atacataagg
    15121 tgcaccgcat ggtacagtaa tctggctaag gcccggagcc tttgccttga gtgtcatttt
    15181 aaacaaaact gacccattgg gcagattttg cggttggaag tttggatcta accatgatac
    15241 ctttaaccct tccgggtaat ttggtgttcc cggtgggatg taatttaggt taaattcctt
    15301 caactgtgga ttaatgtcag aaaaactgac aaaatctagc ttaccggaat caaacaaaac
    15361 cgtaaactcg accgaaatgt ggttaaagtt atcggctgtc acactatacg tgatagtttc
    15421 atctacgtta atggtagcat ctaaggggtt gatcttgatc gtcggtgcca ttgcaaatgt
    15481 ttgtgctact gaaatggcaa aaaagaacag gatcattgaa ataaacttgt tcatgtccta
    15541 ggaattggga agtgattgaa aaggtttttg ttgaaaaatt gttattatca tcaaatatta
    15601 atttcctctt attaattttc ttgtcagaag aatattgtct gacttaagtg ttacaaaata
    15661 tgttccggcg gcaggaaaat ccttacgatt gagtatcagt tggttcttac cctgataata
    15721 acgggcatac ttttggtaaa cctgattacc gttaaggtct gtgactatga tgctggcatt
    15781 accagactga ggcattaccc agcttatttt tgtattctga aggaatggat tagggtcagg
    15841 attgtacaat cggtatttac cttgctcaga atatttttca ttaacgtaca agggtggttt
    15901 tacgtaaacg tacccaatgc ctttgataac cggcacttca tttccaacag aatcataagc
    15961 ttctgcaaaa accggtgaat ctgaaacaaa aactgatgtc gaatcgcctt ttgagccgac
    16021 aacatcaaat ataaaagtca gcatagtact gccatctgcc ataatattgc ccaaaggctg
    16081 gaatgatgtc catagcaagg ctatttttcc ggaagccaag gtatcggggt cagcaccaat
    16141 catatcggct gacaggccag gcagattaaa atttgatact gtatcgtatt tgagtactgc
    16201 cggattccag tttaatgaaa attgtagccc ggtcagctta ttgaagtttt ttactctgat
    16261 gttaacctgt gccttgtcgc ctgcatgggc aaaagacgtg tcgggtgtaa ggtaaagctg
    16321 agcagacatg ctacccactg ctaccgatat ataaaaaaac agggtcagta attttttcaa
    16381 aggattcata caattatatt aataaattca ataattaaaa gcctcaatta agtgcaaact
    16441 gttttggtta atattgtttt gttttggtgt aattgtaaaa atcggcgagc ttagattcat
    16501 gatacgcatg tgttttataa aatgtttctg caatcacgaa tttcttatca acttctctac
    16561 gattcgtttt attcatggga tcataatctg ctaacaacta ccaaggcttc ttccagtgat
    16621 aaaaattaaa gaccctggct tatcagctat actgtttgat aattatgagt gaatgaaatc
    16681 ttctcgcatc aggtgcagta aaataaattt ctgcaatgac atctgctaaa gcagcaaaag
    16741 ggcgaagcaa taaaagtgta atcttttgta tgggcatggg atctcatttt ttaccggcac
    16801 aagaccggac ggtttaagta atatgttcgt tagttgctct attgcataaa ttatgccaaa
    16861 aacattttca atttatgtaa tatgtctcat ctcacattca cacacaaagg tatcggggtt
    16921 tattttaaaa gaccataccc tgtttatggt aaatttgata ttaagaaaaa atgaaaagtt
    16981 tataaaacct cctgactgaa acacattaat atatatacaa aattccttac acttcaattt
    17041 tactcaacct tgtgactaat ttttagacct ccatgaattg atactataac atccgtaata
    17101 ttccttcgaa aactttacac gatgtgtatt tttttaacaa ataactcctt tgtataataa
    17161 ttagaatgtg gcattgaatt atctttgcgt ccagttccat caaaagcata ctatgccaaa
    17221 agactcctcc atccgctccg tactgataat aggcagcgga cccatcatca taggccaggc
    17281 ttgtgagttt gattattcgg gcacacaggc ttcaagatcg ctccgcgaag aaggcatcga
    17341 agtgacactt atcaattcaa atccggccac catcatgact gatccggtca cgacagataa
    17401 tatttatcta atgcctctga ccgttgaatc catcatcgaa atcctcgaaa aacacaaaat
    17461 cgacgccgta ctccctacca tgggcgggca gacagcgctc aacctggcac tcaaagcaga
    17521 agaacaaggt gtatgggaac gatacggtgt acgaatgatt ggcgtggacg tcgatgcaat
    17581 agaaaaagcc gaaaaccgtg aactcttccg gcagttgatg atagagatcg gactagatgt
    17641 agcaccgagc tacattgcca attcgtacct cgaaggcaaa gaagccgcac aaaagatcgg
    17701 ctttccgctt gttatcagac catcatttac gctaggtggt accggtggtg gatttgtaat
    17761 gaaacctgaa gaatttgacg aagccttaaa aagaggactc aatgcgagcc ctacacacga
    17821 ggttcttgtc gaaaaagcgg tactcggctg gaaagaattc gagcttgaac tactacgtga
    17881 caaaaacgac aatgtagtca tcatctgtac agtagagaac ctcgacccta tgggcataca
    17941 tacaggtgac agtatcacgg tggctcctgc tatgaccctg tcagacacag cataccagga
    18001 catgcgcgac aaagccatgc tcatgatgcg ttctatgggc aattttgccg gtggctgtaa
    18061 tgtacagttc gcccaaaatc cggaaactga gcaactcatt gccattgaaa taaatccacg
    18121 tgtatctcga tcatcagcac tggcaagtaa ggctaccggc tatccgatag caaaaatagc
    18181 cgccaaactc gccataggat atacgctcga tgaactcagc aaccaaataa ccaaaacaac
    18241 ttcagcatat ttcgaaccat cacttgacta tgtaatcgta aaaatgcctc gctggaactt
    18301 tgacaaattc gccggtgccg atcacttgct cggactccag atgaaatcag taggtgaagt
    18361 aatggctata ggacgcagct ttacggaagc actacagaaa gcctgtcagt cacaggaaaa
    18421 taatcgctcc ggcctcggtg ccgataaaaa agaatggata aacacccagg atatcctcga
    18481 acgcttagaa aaagcaagtg atgatagaat ttacagagtt aaagacgcac tccgcctcgg
    18541 cattcctgaa aaaacagtgc acaaaatcac actcattgac ccttggttcc tgaagcagat
    18601 aaagtctttg gtaaaaatgg aagagcaact tatgcgctac aatttacctg aagatatacc
    18661 tgccgctttt ttcagagaat tgaaagaaaa tggttactcc gatgcacaga tagcatggtt
    18721 actccgagtg gacgaatcgg cggtaacaag acagcgcaag aaactcaaca tgcttcgtac
    18781 cttcaagatg gtggacactt gtgctgcaga atttgaagct aagaccccgt atttctactc
    18841 aagcttcgat acagaaaatg aatccatccc atctaaaaag aaaaaaatta ttgtacttgg
    18901 ctccggcccg aacaggatag gccagggaat agagttcgac tattgttgtg tacatggtct
    18961 gctggctata aaagaagccg gatacgaggc catcatgatt aactgcaacc ccgaaacagt
    19021 ttctacagat tttgacatag ccgacaaatt atatttcgaa cctgtgtact gggaacacat
    19081 cgaagagata atcgatcttg aaaaacccga cggtgtcatt gtacagcttg gcggacaaac
    19141 tgcacttaaa cttgctgaaa atttgtttaa aaaaggcatc aacatagttg gtacagattt
    19201 taagtcaatg gaccttgccg aagaccgtgg ttcattctcc gacctgttaa aagtattgga
    19261 aataccatat cctaagtatg gtgtagctga tgatgtggac gaagcattaa aaatagcaca
    19321 cgaagtaggt tatcctgttc tggtaagacc ctcgtatgta ctcggtggtc aaaggatgcg
    19381 tatcgtcatc aatgatgatg aactcgagcg aagtgtcctt tcaatattca aacaccttcc
    19441 cggccataat gtactcatag accatttcct agaccgcgcc aaggaagccg agatagacgc
    19501 catctgtgac ggtgatgacg tacacatcat gggaataatg gaacacattg agccggcagg
    19561 catacatagt ggtgatagct ctgcaatgct tcctactttc agccttacac aagaagaagt
    19621 ggaacagatg gctttttatg caaaaaaact cgccttcgca cttaatatca gaggacttat
    19681 caacattcag tttgccatca aaaacggcaa ggtctatgtc atagaagcca atccgcgtgc
    19741 ttcacgcact actccattca tcgccaaagc ctacaaggtg ccgtacctga ccatagccac
    19801 acatatcatg atgggtacaa aaaaactgaa ggattacgac attaaaccac aactcgatgg
    19861 ttatgccatc aaggtgcctg tattttcatt tgaaaaattt ccgggcgtcg ataaaactct
    19921 cggaccagaa atgaagtcaa cgggcgaaat gatttatttc atcaaagatc ttaaagatcc
    19981 atacttcaga gagctcatga atgaacgctc aatgtaccta tactcataag ctgctgtatt
    20041 aagcgataat attgtaaaac ttggaacgtt atttgcaaca cattataaaa aaatataaat
    20101 tatatgttgc gaataacgtt cctaattttc agtatgtgtg cattatttaa ttatgacact
    20161 ttaatggcac agcaattgga tggttttgtt aaaatgggcg gtggtgtcat acaggcatcc
    20221 aacttgaatc ttcaggtaaa gccttttttt atctccaaaa ccgaaatctc caacaaagag
    20281 tatcgtacat ttttgctttg gttagagaaa aatcgttcaa gtgacgagtt ggctgaagct
    20341 acgcaagccc ttgatgtggc ccaaaccaaa ttattgccaa aaaattatct gaaatcttct
    20401 aaatacgata attatccgat agttggcgtg tcgtacagag cagctcagct atactgcgaa
    20461 tatctcaatg ccatacatcc cggtctttct ggaaaattcc gcctgccgga tgaccgcgaa
    20521 tggctctatg cttctgcaat atctaatgac gacatgcttc attcagaaat tgccgcatcc
    20581 aaagataaaa gctataattt caaactgacc acagaaaaaa ctgctccaat gccggtgaat
    20641 agcctgaaag ctaatgggtt cggaatctac aacatggtag gtaatgtagc agaatggacc
    20701 aactcgcccg ggagaataaa aggaggcagc tggaatgata atgttgaaac cttaaatatt
    20761 aaaaaaccgg gtaaatacaa tggtcagaca gcaccttcac cttttattgg attcaggccg
    20821 gtttattttg aagagccggt gaagtaatct gaaaaaattc attgaatgcc acccagctga
    20881 tgcgcgaatt gtacaattct gccaattgac tgaatatttc cagccctgta agcaaatata
    20941 gatgtttgaa ctgcccgata taaagacctt ggtattcaat gtttgacaaa gtgggataca
    21001 ggcgactgta tttcagatat ttaccacgtt gataataagg aaatgacctt acgagagaat
    21061 caagcaacct gtcgatgaaa tgtgaaattt gaatattcgg atacaatacc tcgtattcaa
    21121 gtaatgaaat gatacaaaaa atatatccat taagtacgta tgatggttta gctcccggat
    21181 attcttctat ccaaggcaaa ccttcgggcg tattgatcag aataccgccc tctgctcctg
    21241 gtaccatcat tgactttaca agcttatgga taacaagctc taatctctca tccgggtttt
    21301 gttggttgcg ccttaatgct agactaagca tggtagcctg tgtaataccg gcataccacc
    21361 ttcctttgag taggaacttg ggcagcggaa tattataata tagtataaaa tgatcttccg
    21421 tctctgtacc taatttcagc aatgtatcca attgagcttt tgcgtcaagg agaaatagct
    21481 cttcaccagt ttttagataa gaattgaagc acaccagacc ataataagat ggatagaagg
    21541 ggctgcaatg atccaatttg ctttcgatgc ggtgctgggc tattccatcc ttcattttca
    21601 gatgctgtgc aaggtcatta gggtgatgaa aataactgaa tgtatgatag aacaaatcga
    21661 gcttattcaa tcttttgcgg tcgcaaatct tgactgtatc aggtttaatt ttcggataat
    21721 catctatacc gggcacccat tcggaaatga atccatacag tttcagcaaa atggttttta
    21781 agtaataatt ggttgattta taaggcatta actattattt ctcgtcataa aaatgctggt
    21841 acattacagg gcaactaagc atcattgcaa caatgcaact ctcatattct gtcagaatga
    21901 actatatttg tattccgaag tgtaaggtac ggaattttca tggaagaaaa aaaaataata
    21961 atcaggcccc aaaatgcttt ccggttcaga actgccgagc tgcttgagta ccgtgagatg
    22021 atttattttt tcgcctggcg cgacaccaaa attcgttacc gccacgtctt acttggtatc
    22081 atctgggcta tagtgcagcc cttatttatg gcactcgtgt ttacaggcat tcttcatacc
    22141 ggtcttaagg tacctacagg agacacacct gtgttcctct attttttgtc cggcattatt
    22201 gtatggaatt tcttcagtca gtcggttatt ttttccacaa cttcgatgct tgccaatgct
    22261 catatcatcc gcaaggtgta tttcccaagg cttgccgtac ccttagcagc tatatttacc
    22321 aaactcattg attttgttat cgatctcata ttttttacag ttgtggcctt ggtgtataaa
    22381 tttatattta accacaacat caacattagc ggtgtttggc tggcattgct ccttgcattt
    22441 cctgtacttg ccattttcac gacgagtgtg agtatttttt tatcggggtt aaatgtccgg
    22501 tttcgcgacg tgcgcaattt tataccatat ttcattcaaa tcatgttttt tctcactccg
    22561 gttatttata ctaccagggc gggaaactac ccattgctaa agacaatact ggagctcaat
    22621 ccactcaatt tttgtatcga aatattccgc tcaaatatca ctgcagaaac atttcagctg
    22681 ataactatac catgggctac tgttgtgttg cttatttttt cttatttggc agctatatat
    22741 acttttcgaa aaatggaaga taactttgcc gatatgatat gaaaacagcc attgatgtaa
    22801 aagaaatctg gaaagaatac gtaaaaacgg aaagcagcgg ggctaatctg cgtgaatccc
    22861 tgctccgaat acctcggcaa ttgttcaaaa caagacaaga aaaattctat gcattacaga
    22921 atgtcagttt ttctttagaa aaaggtgaat caatagcttt ggttggcaag aacggtgccg
    22981 gcaagtcaac tatgctgaaa atattggcca gaataacgcc acccacacga ggcgaatgca
    23041 tcatttacgg aaaaatagcc agcctgctcg aggtaggaac cgggtttcat cctgaactta
    23101 cgggaagaga aaatattttc ctgaatggtt cgattctggg tatgaagaaa aatgatatca
    23161 atgcacgctt tgacgaaata gtcgaatttt cgggcgtagc atcattcatt gatacacaac
    23221 tcaagaaata ctcgagtgga atgcagatgc ggctcgcatt ctcagtagca gccttcctca
    23281 atgctgacat actgcttata gatgaggttt ttggtgtggg cgataccgaa tttcagcaaa
    23341 aatcactgga aaaactcaga caaatcagtc aggacaaagg caaatcagtc atttttgtca
    23401 gccatgatct cgacatcctt tcaaagctca ccacgcgggg catcttgctc caggacggct
    23461 gcattaccgc agacaccgat atccattcat gtataagtaa gtaccgggat agtttttgag
    23521 agtcgttttt tcattgcaaa agattggaat gaaaaaaaac tttaataccc gattttctct
    23581 acctcaatat gaccagctta ccaaaaccat gctgcagaaa atcatccgcc ttgcttgtgt
    23641 aactatccca ttgtttgccg gcatcatctt tgatgaccac ctgataaaga taaactccgt
    23701 tcgcaagctt gttgccgtac atatctgtgc catcccattt gtagtctgtg acatgtgtac
    23761 ctaccctaag tatgcccaac tcctgttctg ttatctcttt cacgagcgaa ccggatactg
    23821 agtaaatcct tatgcgcaca tgctcaggcg tacggtcacc ggtgagtgta taaacaaaac
    23881 ccatggcagt cgaaaacgga tttgggtaag gtagaaaacc cgaaacggac ttttggttaa
    23941 tgaccctgaa ccttatctta aaatccgatt gaccggaagc attgttgctt gcatctttcg
    24001 cctgtactag caattcgtat tctccatctt tcagctcggc aggcttgtat atggctttcg
    24061 caatgttgct tttgccggta ccttttgcgg gtataaaact gacttctgag ctgctgatag
    24121 gtatggcatc aaaaacgctc ttacccggat atcttaaaaa taatttgaag ctcgatgtat
    24181 ccgttagtga aatatatttg ttttcatctt ttaactcgat ggttatctcg ggtttgggcg
    24241 acactatatc accatcaaga atgtgtattc cgtcaaataa tacattcaac aaaggatttc
    24301 gcctgtcggg taacaccgta aacctcaatg aggcctgatt attgaaataa taggtttcga
    24361 gttgttctgg tccggggtta atctcgatat ccaatttgta atctcctttg aggtcaatgg
    24421 tgtttcgttg gaaagggaat gtcatttttt ctccggcacc aatggcaggc tgcttcttca
    24481 attctacctg ctggctcagg tctgcagccg tcagcacata tttcaccaat acactgtcag
    24541 aaccaacacc acttatgttc tgaaccggca tgttaagttt cagaatatca ccctggtcaa
    24601 tggtatcttt atgaaaactg taatcggcat ttgcagagtt gagagccagt tctgtcacgc
    24661 cggaatagta aagccttata tatcgcacat aaggtgctgt tttgaataat gtatccttca
    24721 ggttaaccac cacctgcaga tacggatatt ggctggcatc aatactcgaa agatccgctg
    24781 atttagccga cttgaggtca agcagcaatt gtttgttttg ctttgatttg tcgagtccgt
    24841 aaactgcaag gtctgaataa tcgccatctg cagtattttc ttcaatatct gctgttagtt
    24901 tggtccagga tttggcaggc cctgccaatg gtgttgtcac agagcctctg tcccaactac
    24961 cttcaagtcc gaaattgagg cttgccacat catctatatc tttgccaatg gcttccccaa
    25021 gtggctttac gtctttttga tacccaaaag aataaggtac ggcattatta gctgtatttc
    25081 ggattgactt tgctccttgc gcctcgagca actgaaaaat attggtaccc agggtgatac
    25141 tatctgcagc ccaatcttca ggcttgtacg aattcttggc atcctgtacc gtgaaaaaca
    25201 ccacaaagta cccggaaggt attgtgtcct ttagaaatga aatcaggctt gcacgcttgg
    25261 cagggtcggc agtaggaaaa ggaaat
//
LOCUS       DAOQQY010000010        24616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_605_length_24616_cov_5.708522, whole genome shotgun sequence.
ACCESSION   DAOQQY010000010 DAOQQY010000000
VERSION     DAOQQY010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24616
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_605_length_24616_cov_5.708522"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PLE23_01245"
     CDS             <1..320
                     /locus_tag="PLE23_01245"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX81919.1"
                     /translation="IADVIVARAGALTVSELCLVEKPVILVPSPNVAEDHQTKNALSL
                     VRNNAALLVKDQDARTNLFPLAHKLLNDKDTCRAMSVQIGLLARPDAARDIAQVIISN
                     ANI"
     gene            320..1690
                     /gene="murC"
                     /locus_tag="PLE23_01250"
     CDS             320..1690
                     /gene="murC"
                     /locus_tag="PLE23_01250"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HMX81920.1"
                     /translation="MLNLADIRKVFLIGIGGIGMSALARYFKLMGKEVNGYDKTQTAL
                     VTQLIREGINIHFEDSVDLIPTDTDLVIYTPAIPAQHAQFQYFKDNGYSLYKRSQILG
                     FLSNEYKVLAVAGTHGKTTTSSLLTHLLLSGGIDCTAFLGGISESLGSNFVFGKSPWM
                     VVEADEYDRSFLTLHPEHSVITSADPDHLDIYGNEAEMLKTFVQFASQNSKNGQVFIK
                     EGLAITQDVKALHTNVQTYGIGAGEIYASEITIKEGYFHFDYCNNGTSYHDLKISLPG
                     RHNIENAVAAISVASLLGVTEADIRHALVSFKGIKRRFEFIHRGRVSYIDDYAHHPGE
                     LAAAIQAARELYPEARITGIFQPHLYTRTRDFADGFAAVLDTLDEIWLMDIYPARELP
                     IEGVDSKMLLEKMQNPNARLMSRNQILEATSSLEQGLLMTLGAGDIDTLVKPISEILK
                     EKQHEN"
     gene            1680..2453
                     /locus_tag="PLE23_01255"
     CDS             1680..2453
                     /locus_tag="PLE23_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81921.1"
                     /translation="MKINFLSKVRSRYYWMGGILLCTVILFTSISSRKSGIIQDVRIT
                     IKPLQGGELLVQDKDIINTITSNYGSTLDGLAIREIDVQKIEDFAKMNPFVKKAEVFI
                     DARSRLCVNITQNNPLLRIFDKYGHSYYIDEDLKTFPVSRHDSPRMLIANGYLPAQDQ
                     VLSNADSMIIHDVVKIADEISKDEFAQRNTEQIYVEEDGNIVLATKLGNQKFLIGDAS
                     RIEEKLYYIQIYYKNIASTAGWKKYKFVNLKFDGQIVCN"
     gene            2541..3896
                     /gene="ftsA"
                     /locus_tag="PLE23_01260"
     CDS             2541..3896
                     /gene="ftsA"
                     /locus_tag="PLE23_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMX81922.1"
                     /translation="MNKNLTNSKDLTVAVDIGTTKVCAVAAVRNDYGKLEIIGYSTVF
                     SDGVHKGIVKNIDRTVSAISQVIKNVEEQIGRKITKVNVSLSGQHVRSYHQQAIIPRQ
                     NPNIVISDTDVDSLINQMGNVALPAGDKIIEILPQEFSVDNEGGILDPRGMTGVRLEA
                     NFHLITSQHSTQQNLNRCIEMAGLTIGKTMLEPMASAEAVLTDEEKGAGIALVDIGGG
                     TTDIAIFKDGLLRHTAIIPLGGNNITNDIKEGCEVLQPQAEKLKVRFGCALAQEINEN
                     RIITVQGIRGHEPRAVSEINLARIIQSRMEEILDFVGSEIRRTGYERKLIGGIVITGG
                     GALLNHIRNLAAYRLGMSVRIGSPRESLAQGYHEEISSPMYATAIGLLLKGVPDMTPV
                     ANEVMVEDLQPDTSDTDAQNTIPYHEPDEAGDTDIFKDPKNWFDNLVNRATSWFTETK
                     D"
     gene            3941..5350
                     /gene="ftsZ"
                     /locus_tag="PLE23_01265"
     CDS             3941..5350
                     /gene="ftsZ"
                     /locus_tag="PLE23_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMX81923.1"
                     /translation="MRFVMQNDKKSIIKVIGVGGGGSNAVNHMFKKGIEGVDFAVCNT
                     DNQALDQSPVPVKIQLGPSLTDGRGAGSKPNVGKQACIESKAEIEKFLATGTKMLFVT
                     AGMGGGTGTGAAPEIAKIAQEMDILTVGIVSLPFSFEGNKRITQGNEGLEELKKNVDT
                     LVVISNDKMRSIYGNLSMSEAFGKADDILTVAAKGIAEIITVAGYINVDFEDVNTVMR
                     NSGVAIMGTASAEGDDRAKKAVDMALTSPLLEDGCIQGAKHILLNITSGSKEVTMDEI
                     TQITDFVQTEAGSDANLIWGNCFDESLGNQIAVTIIATGFDHTDSEKKQDDKTGVIVV
                     PLDGDDNTPSISSISSEGNKGQPIDLEPTRRFNPNPYYPDEVYVSVGNKNAQQYGTDQ
                     KPKSFMSKADYDSITELTNTGYERGFKLYEDEPAFKRKNVNLETVSSHTEEFTSNYSI
                     NESGEAVFGPNKYLHGRPD"
     gene            complement(5419..6294)
                     /locus_tag="PLE23_01270"
     CDS             complement(5419..6294)
                     /locus_tag="PLE23_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HMX81924.1"
                     /translation="MNKISEADLIINKDGSIYHLNLVPDDLADTVITVGDQDRVGKIT
                     RMFNKVHLKKSNREFVTHTGELNGKLISVISTGIGTDNIDIVMNELDALVNIDFATRT
                     VKENQKSLEIIRVGSSGSMQPDLDTGTLLISEFGIGLDGLLLYYDYKMNKKEVALHDS
                     FKSFCDRALKLPMNFYTAMADETLVSRFSDTFVKGITVTCPGFYAPQGRKVRAHNPNT
                     DYLDTLSNFSTGQLRTTNFEMETAGIYGLGSVLGHRCLSCNVLIANRKTGKFSQDPQK
                     DIEKLIDAVMEKIIM"
     gene            complement(6512..7003)
                     /locus_tag="PLE23_01275"
     CDS             complement(6512..7003)
                     /locus_tag="PLE23_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81925.1"
                     /translation="MSWYQLFAFTLLVFCLSVVGCTKPPDYPIEPVIKYLGMSKKQLK
                     QSSINADSLRVSFSFTDGDGDIGSNDSLSLFVVDKRDGFLSSKYKIPFITEAGAKNGI
                     SGEIEFVLFSTCCYFPDGQAPCTPSKTFPTDTVVYEIYLKDRAGHKSNIIETEPIILD
                     CTK"
     gene            7073..8047
                     /locus_tag="PLE23_01280"
     CDS             7073..8047
                     /locus_tag="PLE23_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl transferase"
                     /protein_id="HMX81926.1"
                     /translation="MNSFSSSPTDFDKKALTLFRKQATENDFYRRFLEILKVQPQNID
                     QVSNIPFLPVQLFKHNEIKTGDWLEQNVFLSSGTTLHTRSRHFVKDEQKYLRNAATGF
                     KEVFGNPSEWNIIACLPGYSDNPSSSLISMVHYLIGLSGNIQSGFIPFEHETIKHKLK
                     ECIKSSKKTMLIGVAFALADFALCNKVEYADLYILETGGMKGRGEDFTRKALHTLLSD
                     SFGTAHIFSEYGMTELFSQAYTMAGTDRFKCMPSMKILVKDINDPMEIKRNGRYGAIH
                     IIDLANEDTCAFIATQDLGLLHDDDSFEVIGRIDSADLRGCNLLYIAP"
     gene            8103..9998
                     /locus_tag="PLE23_01285"
     CDS             8103..9998
                     /locus_tag="PLE23_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HMX81927.1"
                     /translation="MKNETFYLRQLLYRLGIIFLLLTLIRLLFLWANFSTFSVFPTNK
                     LLLSFLVGLRFDISSLLYTNALFALLAILPFGFRSNKNYQHLLFIVFLGLNAVMLALE
                     IPDIAYFKYAQRRSGVGEFNLAGDFVRLIPAFIKSYWYLWILFGLLVYGLSRIYKKWY
                     FNVVVPPTRWYWQWIIFILTMGAVVIGMRGGLQLRPMTPVSAGVVLKDARLIPLATNT
                     TLNFIFSFQQKALTELQYFPVNELDARFALKRNVAATAPFQPVNVVLIILESFGTEYI
                     SRQSDGKIYAPFFDSLATQGISFGQSYANAQRSAQGIVSVTSGIPALMEEPFHFSPYQ
                     SNQIESIASALKQKGYYSAFFHGSNPGSMDFERFAAISGYDDFFDRRDYSNDRDYDGS
                     WGIWDIPFFQWTVSQMNSFKKPFFCTLFSLNPHNPFKAEKWFEDKYPGENPTLRCVRY
                     TDEALRQFFKTASGYDWYQNTLFVLCADHTGVPFGEKYTTREAIFRIPIVFYSPNTSI
                     ISKAQIGVKPNEALMSQIDILPSILDLLHYDHDFHCFGESIFRHDKKKYTYQYSNGVF
                     QILDGEYILLFDGSNVAGLYNYKQDPMMRNDISSKETELTKQMSDQLKTVLQRYNRAM
                     IRNELNR"
     gene            10528..13056
                     /locus_tag="PLE23_01290"
     CDS             10528..13056
                     /locus_tag="PLE23_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81928.1"
                     /translation="MLDNIPPVAVCKSHVQISLTDNPTKVFPVSFDDGSYDEECLAQL
                     LVKRMNEPESAFKPFMYFTCDDIANSPVKVVLRAVDCEGNVNDCWSEADVEDKTKPVV
                     VCPADLTIFCTQDPNQYFTQPTAIDNCGTLTFTSNVIDNRDQCQNGYVSKIWTVKDNA
                     KNQGTCTHRVYSKHVSDYTVTFPSDVTINKCVSPDDLTKTGEPIFKDKQCDLLAVGHT
                     DHDLPVTDGNGCFVRIRKWQIINWCTYDPNKATHTPLGIPIGNKKYKDDDGFFEYTQY
                     IKVFETADPVLTCKDTTICFQNDCEVSFDIPKPAVVDCSQNIYYSLSGDLGSKYSVSG
                     VKSGVYQIQHNVSDGCGNHSFCNVNITVKDCKKPTPVVHQKLATTLMVQNGLVEVWAS
                     DFETSSYDNCTKSENLKFSFSPDKNDISKIFNCDSLGLRTVKIWVTDEAGNQDYAIAT
                     IDIQDNMNACDTSGTIIQVSGSVKKPDGSPMVDVHIKNPYVEVITDSVGYFATPLLNM
                     HLPYTMECIKQEDPKAGLSVLDAILLRDHILGYKKLSNPYQYLAGDVDKSKGINTYDL
                     FLLKQMILGHLEEWPKNQTFNYISKKKTFDKPEEIWNDATTGLLLDSLATDSLVFDLV
                     AIKTGDLSDADQLIGSDNISTRASTTISFVSGNKINYTGDGPAKGIQFAVNIKALSTE
                     IYAEQGTISITDVDNGMFEVKYITDEVVIPGQTLLSWHNTDKNFLPVLTNSLQPMSVA
                     ENNELQQLDLKHPKVNFPLEEGIFVYPNPAKNAVRVKITSAGAQMATIEVIDLEGKVI
                     DHFDKNLIEGVNLYALPINTGTGIFIIKVETQRNIFQEKISIIE"
     gene            13089..14258
                     /locus_tag="PLE23_01295"
     CDS             13089..14258
                     /locus_tag="PLE23_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81929.1"
                     /translation="MISLEGRVPVSLNKLQLLNYLQAEVRLHHVSPYTSRLLTIESEL
                     QHFQMLDFNISSPANQLIVDIEMLIPVNVRLPLCKFRVNTKPKIKISILGTGFSGTSK
                     IESLSLSLRTLQLNGALSVFEGMIRKNIIAQVEKLLPDILKLVNSKISELLSQKTQID
                     FNISGIDYNNLLYVKGVKLRECSSSNNSLLIDVFSSAYIENQNQGFGAEYYVIERKEH
                     TGQFNVAIHQDFLKSILTKLITEVEIPVLNKKIPLVLTDFRVVCNVLYVGLELSEFYK
                     GAWRLKLDTSVTSDTLRFSLLEMQSTGSNSLFDKGITKLLELGIEKLIQKYAEIPFST
                     INAHIAGGIKDAYHFDFQSSAIKLSVPKFSVKHITFDDKIVLVNCEWQDRFYLEL"
     gene            complement(14253..14642)
                     /locus_tag="PLE23_01300"
     CDS             complement(14253..14642)
                     /locus_tag="PLE23_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HMX81930.1"
                     /translation="MVRKRNILLFLVAFITLIFVLINLGGFKKYIILGNWVGAHVAEN
                     GNSIKMDWSKVKFTFKLNGDYSYTNPSEYTEHGKYKLEKNMLITQPSNIQTNQPVAVE
                     IIQLRLTSLILRMNDSGNEIILQLKRL"
     gene            14840..15838
                     /gene="dusB"
                     /locus_tag="PLE23_01305"
     CDS             14840..15838
                     /gene="dusB"
                     /locus_tag="PLE23_01305"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMX81931.1"
                     /translation="MVKIRNIEIADFPLILAPMEDVSDPPFRFLCKEEGADLMYTEFI
                     SVEGLIRDADKSLRKLDIYENERPIGIQVFGGEIDSMVEAMAVVEDADPEIIDINFGC
                     PVNRVVCKMAGAGILQDIPKMIKLTESVVKATSKPVTVKTRLGWDENSINIIEVAERL
                     QDVGIEALTIHGRTRKQMYKGQADWSYIGKIKENPRMHIPIFGNGDIDSPDKAKIYRE
                     RYGVDGIMIGRASIGYPWIFREVKHYFKTGELLVAPTIAERADAASRHLQHAIRWKGE
                     KLGILETRKHYTNYFKGFPDIKPFRARLVTADKPEDVFAVIHEMKDKYSVTEVFMS"
     gene            15910..17280
                     /locus_tag="PLE23_01310"
     CDS             15910..17280
                     /locus_tag="PLE23_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMX81932.1"
                     /translation="MQKAAELLNIQAYLVGGFVRDRLLGRPCKDLDIVCTGDGILLAE
                     TAASMLPGKPKVSVFQRFGTAMLRFRDMEIEFVGARKESYVSDSRKPYVEAGTIEDDQ
                     QRRDFTINAMAISLQKEDFGTLVDPFNGVQHLEEKVIRTPLEPRKTFSDDPLRMLRAI
                     RFSAQLQFEIFPETLASITDNCDRILIISQERIIVELEKILLSIKPSVGFEQLYKTGL
                     LKHILPELVELQGVEYIDGKGHKDNFYHTLEVIDNLRSNTDNIWLLWAAVFHDIAKPA
                     TKKFTPDTGWTFHGHEYLGSVMLPRIFRKLKLPMDGKLKYVQKLVSMHLRPISLTKEN
                     ITDSAIRRLLFDAGDDIDDLMLLCEADITSKNAEKKARFLENYRVVKQKLIEIEEKDN
                     IRNWQPPISGELIMSTFNIKPGREVGLIKNAIREAILDGVIANEYETAYKLMMEKGAE
                     LGLVIV"
     gene            complement(17375..18391)
                     /locus_tag="PLE23_01315"
     CDS             complement(17375..18391)
                     /locus_tag="PLE23_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81933.1"
                     /translation="MASITPPSDFKPKTFWQRPEGVTGGIFMTGIIVGLGYLLYKMLP
                     ILITLVSNTLYLALLLIALAAVIYMVVDPRMRNLFWYGYKSIMRWITGIFVNLDPIGI
                     LKSYVEDLENNLSKMSKQIGNLRGSMRKLKTTMEENDKSIENELNIANAAKKQGNEKQ
                     LVLSTRQAARLKDSNEKYNSLYSKMDILHRILAKMYENSEILLEDTRDQVKVKQEERE
                     AIRASHSAMSSAMKVISGDKDKKAMFDMAVEAIADDVANKVGEMERFMDMSSKFMDSV
                     DLQNGVFEEEGLKMLEQWENQSQLLLMQGGKSDTLDLNNHPEMLPRENDKKEDGDSYK
                     KLFD"
     gene            complement(18459..19430)
                     /locus_tag="PLE23_01320"
     CDS             complement(18459..19430)
                     /locus_tag="PLE23_01320"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMX81934.1"
                     /translation="MPIIDLSKNYKPNWYSNNRHLATIVPSMTRKIDNIQYVRERLTL
                     ADGDFLDLDWIKKANDKLLIITHGLEGNSNKQYVKGMAQEFSINGWDILAWNCRSCSG
                     EMNIAPKLYHHGDIHDITEVIEHVLDKYKYSQIALGGYSMGGVINTKFVAKKAVLYSD
                     FIKLNIAISTPFDLEACAHTLDRKANFVYKNKFMKSLMVKLSEKEKQYPGIIDKAGLS
                     NIKSWAQFDEQYSAPFNGYNSRQELYANATINNWLEDIRVPTLLLNSVDDPMIPVESN
                     PKQFAQSSDILTLALTPKGGHCGFGQRQSEKSFAESFSFAFANKILK"
     gene            19478..19696
                     /locus_tag="PLE23_01325"
     CDS             19478..19696
                     /locus_tag="PLE23_01325"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HMX81935.1"
                     /translation="MQIAKGLIIAGVILIIIGLIYYFFQDKLGWLGRLPGDFSYKKDN
                     FSFFAPITTMLILSILLSVIINLIRRFL"
     gene            complement(19707..20999)
                     /gene="tyrS"
                     /locus_tag="PLE23_01330"
     CDS             complement(19707..20999)
                     /gene="tyrS"
                     /locus_tag="PLE23_01330"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMX81936.1"
                     /translation="MKKNFVEELRWRGMLQDIMPGTEELLNKEVVKAYIGFDPTADSL
                     GVGNLVQIMTLLHLQQCGHIPIALVGGATGMVGDPTGKSEERNLLNQDTLNHNLACQK
                     KQLEKFLDFESKTNPAEIVNNYDWFKEISFLDFIRDTGKHITVNYMMAKDSVRKRLDS
                     EVGMSFTEFTYQLIQGYDFYYLWKNHNIKLQMGGSDQWGNIVTGTELIRRKAQGTAYA
                     ITTPLIKKADGTKFGKSESGNVWLDPKRTSPYKFYQFWLNASDEDVSTYIRIFTLKTK
                     DEIEELENQHKEAAHLRILQKELGKDITCRVHSAQDFESVLKVTELLFNPKLDEDYLK
                     RISEEEWETVGAEIQCYEFNSSSALDQTDLLTLLSDASGILPSKSEARRAITGNAISI
                     NKQKMSTIEAKIDSHALLCGKYLFIENGKKNKCLLKFK"
     gene            21093..22403
                     /locus_tag="PLE23_01335"
     CDS             21093..22403
                     /locus_tag="PLE23_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX81937.1"
                     /translation="MKRTTFLSGFLAGRLSAVRERNFSSLIVRVGVVATAISIAVMII
                     ATCLISGFQKSISEKIFGFWGHIHIIHNSELNSYESKPISVRQNFYPELKDKGPINYT
                     GPQEVLGFVFPNWQTQHTTKGKIRNIQAFAFKAGIMKTKSEIEGIVLKGVDEHFNWTF
                     IKDFIIRGKPVANIGKGSRDILISEQTADRLKTDVGKSLILYFVNNGEQIKKKFTVSG
                     IYKTGLEEYDRKFAIIDIAIIRELMGWAPDEVGGFEVFLDDVADLDAYNTYLYDQVVP
                     DSLYTESIRMKLPNIFEWLELQNTNEQLIIGLMMIVAIINLSTALLILIVDRTNMVGM
                     LKALGASNFLVQKVFIRFAGHILVRGLFFGNLFGLLIAGAQKYFKLIKLSEKDYYLSY
                     APIYFDWSKIMMINVFAFLVVILIMLIPSLMVRSISPLKAIIMK"
     gene            22711..23661
                     /locus_tag="PLE23_01340"
     CDS             22711..23661
                     /locus_tag="PLE23_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HMX81938.1"
                     /translation="MIRYFRKEHGQLMELENFAPGCWVHISTPFSSNELEDFAERFDI
                     ELDYLTDSLDPEERSRYEKEDHFKFILINTPIPNQRDDEYQPVFMTVPIGIIFAEEFM
                     ITISAFDNTIMDLFLKDKVRHFNPGDYQLFILQILDQNVNRFTSYLKKLNLNRDVIEQ
                     QLITSSRNNELKQLLGLEKSSIYFVNALSSNELLKLKLKRTDFLGINGDEDKVDFFEN
                     IIIDNNQAHQMANVFTHIINGTTDAMASIISNNLNVIIQRWTMITIVLMVPTLIASFY
                     GMNVKHLPFDDSDYAFYYIVILSLVLTLPLVWVIKRKELF"
     gene            24163..>24616
                     /locus_tag="PLE23_01345"
     CDS             24163..>24616
                     /locus_tag="PLE23_01345"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMX81939.1"
                     /translation="MQKFNTIDEYHDQQSGAIQVILQLLRQAIRQAAHQATEVISYGM
                     PAFKQNKVLVYYAAGKNHIGFYPTPDPIVHFKKELEGYKTSKGAIQFPISENLPWDLI
                     RRIVEFRLVTDTALAKSTSSKLRNVTEKPTDIPAYHAIQNPVHKEICEI"
BASE COUNT         7605 a         4711 c         5058 g         7242 t
ORIGIN      
        1 ctatagcaga tgtgattgta gcccgggccg gagcacttac agtttctgaa ctctgcctgg
       61 tcgaaaaacc ggttattctg gttccgtcgc ctaatgtagc cgaagatcat cagaccaaaa
      121 atgcactttc tctggtacgg aataatgctg ctttactggt taaagaccaa gatgcacgaa
      181 cgaatctttt tccactggct cataaattat taaatgataa agatacctgt agggcaatgt
      241 ctgtacagat tggtttattg gcccggccag atgcggcacg cgatattgca caagtaatca
      301 tttcaaatgc taatatctga tgttaaactt ggctgacata cggaaagttt ttctcatcgg
      361 tatcggtggt atcggcatga gtgccttggc tcgttacttt aagttgatgg gaaaggaagt
      421 gaacggctac gacaaaacgc aaacagcatt ggtcactcaa cttatccgtg aaggcatcaa
      481 tattcatttc gaagactctg ttgatcttat tccaactgat accgacctcg ttatctacac
      541 gccggccatt ccggcacagc acgcgcagtt tcaatatttc aaagacaatg gatattcact
      601 ttacaaaagg tctcagatac tgggtttctt gtcgaatgaa tataaggtac tggcagtggc
      661 aggtacacat ggcaagacta caacaagctc tttgctcaca catttgttgt tgtcgggtgg
      721 catcgattgt actgctttct taggtggcat ttctgaaagt ctgggctcta actttgtttt
      781 tggtaagtca ccatggatgg ttgtagaagc agatgagtac gaccggtcgt tccttacact
      841 acatcccgaa cactctgtca ttacttcagc agaccccgac catcttgata tttatggcaa
      901 tgaagctgaa atgctaaaaa cttttgtcca atttgcatcc cagaatagca aaaacggtca
      961 ggtttttatt aaagaaggcc ttgcaataac acaggatgtg aaagcgcttc ataccaatgt
     1021 ccaaacttat ggtataggtg caggtgaaat ttatgcttca gaaattacga ttaaagaagg
     1081 atatttccat tttgattatt gcaataatgg aacaagctat catgatctga aaatatctct
     1141 tcccggaagg cataatattg aaaatgcagt agctgccata tcagttgctt cattacttgg
     1201 tgtaaccgaa gcagatatca ggcatgcact cgtcagtttt aaaggcatca aacgcaggtt
     1261 tgaatttata cacagaggca gagtttcgta cattgatgat tatgcacacc atcctggcga
     1321 gctggcagca gctatccagg ccgctcgcga actttaccct gaagcccgga taacaggaat
     1381 atttcagccg catctttata ctcggacgcg cgatttcgct gacggatttg cagccgtatt
     1441 ggatacgctc gacgaaattt ggttaatgga tatttatccg gccagagaat tgcccattga
     1501 aggagtagat agcaaaatgt tactggagaa aatgcaaaat ccgaatgcca gattaatgtc
     1561 aagaaatcaa attcttgaag ctacatcttc ccttgaacaa gggcttctga tgactttagg
     1621 tgcaggagat attgatacac ttgtaaaacc catttcagaa attttaaaag aaaaacagca
     1681 tgaaaattaa ttttttatcg aaagtcagat ccagatatta ctggatgggt ggaatattac
     1741 tatgtacagt gatattgttt acgtccattt cttcgcgcaa aagcggaatc atccaagatg
     1801 taaggataac aattaaaccc ttacaaggtg gagaattgtt agtacaagac aaagatatca
     1861 ttaataccat taccagcaat tatggatcaa cccttgatgg tctggccata cgtgaaatag
     1921 atgttcagaa aattgaagat tttgcaaaaa tgaatccatt tgtaaaaaag gcagaggttt
     1981 ttatagatgc cagaagccgc ctgtgtgtca acatcacaca aaataatcct ctactcagaa
     2041 tatttgataa atacggacac tcgtattata tagatgaaga cttgaaaaca tttccggttt
     2101 cgaggcatga ttctccgcgt atgcttattg ccaacggata cctgccggcc caggatcaag
     2161 tattaagcaa tgcagacagt atgatcatac atgatgttgt aaagatagcg gatgaaataa
     2221 gtaaagacga gtttgcacaa cgcaatacag agcagattta tgtcgaagaa gatggcaata
     2281 ttgtattggc caccaaactt ggaaaccaaa aattcttaat aggtgacgcg tcaagaatcg
     2341 aggaaaagct ttactatata cagatatatt ataaaaatat agcatctaca gcaggctgga
     2401 aaaagtataa atttgtcaat ttgaaatttg atggtcaaat agtctgcaat taatttagaa
     2461 attattaaaa attataatat attgtattaa ttttaccgct gaattataca tccatagaaa
     2521 caacaataaa ttaaagccgc atgaacaaaa acctgacaaa tagtaaagat ttaactgttg
     2581 ctgttgatat aggcacaacc aaagtctgcg ctgtagcagc agttcgtaac gattatggca
     2641 aacttgaaat tatagggtat agtactgtat tctcagacgg tgtacataag ggaattgtta
     2701 aaaacatcga ccgtacggta agcgccatct ctcaagttat taaaaatgtt gaagaacaga
     2761 taggtagaaa aatcaccaag gtcaatgtaa gtttaagtgg tcaacatgtt agaagttacc
     2821 atcaacaagc tattatccca cgtcagaatc ctaatatcgt catttctgat accgatgtag
     2881 atagccttat taatcaaatg ggaaatgtgg cattgcctgc aggtgacaaa ataattgaaa
     2941 tcttaccaca agagttcagc gtcgataacg agggaggtat cttggatccg cgaggcatga
     3001 caggagtcag gcttgaggca aattttcatt taattacaag ccaacattct actcaacaga
     3061 acctaaacag gtgtattgaa atggcaggac ttaccattgg taaaaccatg ctcgaaccaa
     3121 tggcatcagc agaagcagta ttgacagacg aagaaaaagg ggcaggaata gccttggtag
     3181 atattggagg tggaacaact gatattgcaa tattcaagga tggccttcta cggcacacag
     3241 caattatccc attaggaggt aataatatta caaatgatat caaagaagga tgtgaagttc
     3301 tgcagccaca agccgaaaaa ctaaaagtaa ggtttggctg tgcacttgcc caggaaataa
     3361 acgaaaacag gatcataaca gttcaaggaa tacgaggcca cgaaccaagg gctgtgtcag
     3421 aaattaacct tgccaggatc attcagtcca gaatggaaga aatactggat ttcgtaggtt
     3481 cggaaatcag acgtaccgga tacgaaagaa agctcatcgg tggcatagtt attacgggtg
     3541 gtggagcatt gcttaaccac atacgcaatc ttgcagcgta ccgtttgggt atgagtgtaa
     3601 gaatcggatc tccgcgtgag tctcttgccc agggttatca tgaagaaatc agtagtccta
     3661 tgtatgcaac agccattggc cttttgctaa aaggcgtacc tgatatgaca cctgtagcaa
     3721 acgaagtgat ggtcgaagac ctgcaaccag atacaagtga cactgatgca caaaacacaa
     3781 taccatatca tgaacctgat gaagcaggag atactgatat ttttaaagat cctaagaatt
     3841 ggtttgacaa tttggtaaac agggccacga gttggtttac tgaaaccaaa gattgaactt
     3901 aaaaataatg ttttagttaa caacaaatat taatcctgaa atgagattcg taatgcaaaa
     3961 tgataaaaaa tcgattatca aggtaatcgg agtaggtggt ggaggtagca atgctgttaa
     4021 ccacatgttc aaaaaaggca tcgaaggagt tgacttcgcc gtctgtaata cagataacca
     4081 agcacttgac cagtcaccag tacctgtaaa aattcagctg ggacctagtc ttacggatgg
     4141 tcgaggtgcc ggctccaaac ctaatgtcgg caaacaagct tgtattgaat caaaggcgga
     4201 aattgaaaaa tttcttgcaa ctggtacaaa aatgctattt gtcactgctg gtatgggagg
     4261 aggtaccggc acgggtgcag caccagaaat tgccaagata gcccaagaaa tggatattct
     4321 tactgtcggc atagtgtcat tacctttttc attcgaaggt aacaaaagga taacccaggg
     4381 caatgaaggc cttgaagagc tgaagaaaaa tgtagatacg cttgttgtca tctctaatga
     4441 taaaatgcgt tctatctatg gtaatctgag catgtcggaa gcctttggta aggccgatga
     4501 tatccttact gttgcagcaa aaggtatagc tgaaataatc acagtggcgg gctatattaa
     4561 tgttgacttt gaagatgtaa acaccgtcat gcgcaatagt ggtgtagcca taatgggtac
     4621 cgccagcgcc gaaggtgatg accgcgctaa aaaggccgtt gatatggcac ttacttcacc
     4681 attactcgaa gatggttgca ttcaaggtgc aaaacacatc ctcctaaaca ttaccagtgg
     4741 ctctaaagaa gtgactatgg acgaaattac acagatcact gatttcgtac aaactgaagc
     4801 aggctcagat gccaatctca tctggggtaa ttgttttgac gaatctctcg gtaatcagat
     4861 agctgtaact atcatcgcta caggctttga ccatacagac tcagaaaaga aacaggatga
     4921 caaaactgga gtaatagttg ttcctctgga tggtgatgat aatacaccaa gtatctcatc
     4981 aatcagctct gaaggcaata aaggccaacc tattgattta gagcctacac gccgatttaa
     5041 tcccaatccg tattaccctg atgaagttta tgtaagtgta ggaaataaaa acgctcagca
     5101 gtacggaaca gatcaaaaac caaaaagttt catgtctaaa gctgattacg acagcataac
     5161 agaattaact aatacaggtt atgagcgagg ttttaaatta tacgaagatg aaccggcgtt
     5221 taaaagaaaa aatgtgaacc ttgaaacggt aagtagtcac acagaagagt ttacatctaa
     5281 ttatagcatt aacgaatcag gagaagcggt atttggccca aataaatatc tccacggcag
     5341 acctgattaa tgtatatata aaaaggtggt tgaaatcatt caaccacctt ttttgttgat
     5401 cttatacctt gcccaaggtc acattataat tttttccata acagcatcta ttaacttttc
     5461 tatatccttt tgtggatctt gagaaaattt tccggttttc ctgttagcta tcaatacatt
     5521 acaagataag catctgtgcc ctaaaacaga cccaagccca taaatgccgg cagtctccat
     5581 ctcaaaattg gtagtacgta gctgaccggt gctgaaatta ctcaacgtat caagataatc
     5641 cgtattcgga ttgtgggctc tgacttttct cccctgaggc gcataaaatc ccggacacgt
     5701 tacggttatt cctttgacaa atgtatctga aaaccgactg accaaagttt catcggccat
     5761 tgctgtgtaa aaattcatgg gtagttttaa cgctcggtcg cagaatgact taaaactgtc
     5821 atgtaacgcc acctcttttt tattcatttt gtaatcgtaa tacaaaagaa gtccatcaag
     5881 tccgatgcca aattctgata tgagaagcgt acctgtgtcc aggtcaggct gcatgcttcc
     5941 agagctgcca acgcggatga tctccagact cttttgattt tccttaacgg tgcgcgttgc
     6001 aaaatcaata ttgaccagag catccagttc attcataaca atatcgatat tgtcagtacc
     6061 aatacctgta gaaataaccg aaatgagctt tccgtttaat tctcctgtat gtgttacaaa
     6121 ctcccgattg ctcttcttga ggtgaacttt attgaacatc cgggtaatct ttcctacacg
     6181 gtcctggtca ccaaccgtta taactgtatc cgccaaatca tccggcacaa gatttaaatg
     6241 ataaattgat ccgtctttgt tgatgatgag gtctgcttct gagatcttgt tcatgctact
     6301 ttcctttgat taaaaatgtt agtatggcag aatttgatga taatgcttgc ctgtccaaaa
     6361 ttaagcattt tgtgtcaaac aacagacttt tttatctttt attttgaata taccacattg
     6421 cactgtagtt gtatggaatt ttgttcttta atatggcttg gtattttttt tgaatttgaa
     6481 tttattcttt aactgccgac acaacagatg gtcattttgt acaatcaagg atgatgggct
     6541 cggtctcaat tatattgctc ttatgacctg ctctatcttt caaataaatt tcataaacca
     6601 ctgtgtctgt cgggaaagtt ttagacggtg tacaaggcgc ctgaccatca gggaaataac
     6661 aacatgtact gaataataca aattcgattt caccagaaat gccgtttttt gctcctgctt
     6721 cggttataaa aggaatctta tatttgctgg ataaaaatcc gtcacgctta tccaccacaa
     6781 acaaactcag cgagtcgttg ctacctatat caccatcacc atctgtaaag ctaaagctta
     6841 ctcgaagcga atcggcattg atactactct gctttagttg ttttttactc atacccaaat
     6901 acttgatgac cggctctatc ggataatctg gtggctttgt gcacccgaca acagacaaac
     6961 aaaaaacaag aagtgtaaaa gcaaataatt ggtaccaaga catatatatc aaacgttaag
     7021 atgcagaaaa tagtctgctt catattattt ttgtaaaatt aataaaaact ctgtgaattc
     7081 tttttcttct tcacctacag atttcgacaa gaaggcatta acattattca ggaaacaggc
     7141 aacggagaat gatttttacc gcagatttct cgaaatattg aaagtacaac cacaaaatat
     7201 tgaccaagtt agtaatattc cctttctgcc tgtacagtta ttcaaacaca atgaaataaa
     7261 aacaggtgac tggcttgagc aaaatgtctt tttgtcaagt ggtactactt tgcatacacg
     7321 cagccgtcat tttgtaaagg atgagcaaaa gtatctccgg aatgcagcaa caggttttaa
     7381 agaggtattc ggaaatccga gcgaatggaa tattattgcc tgtctgcccg gatattccga
     7441 taatccttca tcatctctca tcagtatggt tcattatttg ataggcctga gcggcaatat
     7501 tcagagtggt ttcataccat ttgaacacga aacgataaaa cataagctga aagaatgcat
     7561 aaaaagctct aaaaagacaa tgctcatcgg tgttgctttt gccttggccg atttcgcact
     7621 ttgtaataag gtagaatatg ctgatttgta catcttagaa acaggcggaa tgaaaggaag
     7681 aggagaggac tttacaagaa aagcattaca cacattgttg tcagacagtt ttggtactgc
     7741 acatatattt tcagaatatg gcatgacaga attgttctca caagcatata caatggcagg
     7801 taccgacaga tttaaatgta tgccgtctat gaagatactg gtaaaagaca tcaacgaccc
     7861 aatggaaata aagcgcaacg gcaggtatgg tgcgatccac atcatcgacc ttgccaatga
     7921 agatacctgt gcatttatcg ctacccaaga tcttggtttg ctgcacgatg acgatagctt
     7981 tgaagtcata ggaaggattg attcggcaga tcttcgaggc tgcaatttgt tgtatattgc
     8041 gccataattc aaaaattccc tgccaataac acatctttgt atgttgatat caacttctgt
     8101 gcatgaaaaa tgaaactttc tacctcagac aattgcttta ccggttaggc atcatttttt
     8161 tgctattgac gctcatccgg ttgctttttc tttgggccaa tttttcaact ttttcagtat
     8221 ttccgactaa taaattgtta ctttcatttt tggttggcct taggtttgat atttcttcat
     8281 tgctctatac caatgcattg tttgccctac tcgccatttt gccgtttggc ttcaggtcaa
     8341 ataaaaatta tcaacatttg ctctttatag tgtttcttgg gttgaatgct gtgatgctgg
     8401 cacttgagat accggatatc gcttacttca aatatgcgca aagacgaagc ggagtgggtg
     8461 agtttaacct ggcaggtgat tttgttcggc ttattccagc gtttattaaa tcttactggt
     8521 acctatggat tttatttggc ttgctggttt atgggctttc gcggatatac aaaaagtggt
     8581 atttcaatgt tgtggtgcca ccaaccagat ggtactggca atggatcatt tttatactta
     8641 ctatgggggc agtcgtcatt ggtatgcgcg gagggcttca gctacgccca atgacaccgg
     8701 tgagtgcagg tgttgttctc aaagacgctc ggcttattcc acttgctacc aacacaacac
     8761 tcaacttcat attttcattt caacaaaaag cacttacaga gctccaatat ttcccggtaa
     8821 atgagctgga tgcacggttt gcactgaaaa ggaatgtggc tgccacggca ccctttcaac
     8881 ctgtgaatgt ggtactcatc atactcgaaa gttttggaac agaatacatt tctcgacaat
     8941 ccgacggcaa gatctatgca cctttctttg atagcctggc tacacagggc ataagctttg
     9001 gtcaaagcta tgccaatgcc caaaggtcag cccaaggaat agtctctgtt acatccggta
     9061 ttccggcctt gatggaagag ccatttcatt tttcgccata ccagagcaac cagatagaaa
     9121 gcatagcttc tgcacttaaa cagaaaggat actactcagc attctttcat ggcagcaatc
     9181 cgggttcgat ggattttgag cgttttgctg ccattagcgg atatgatgat ttttttgaca
     9241 gaagagatta cagcaatgat cgcgattatg acggtagttg gggaatttgg gatattccgt
     9301 tttttcaatg gacggtaagc caaatgaatt cattcaagaa gccattcttc tgtaccttgt
     9361 tttcacttaa tccacacaat ccttttaaag ccgaaaaatg gttcgaagac aaatatcccg
     9421 gtgagaatcc taccttgcga tgtgtgcgtt acacggacga agcactaaga caatttttca
     9481 aaacagcttc cggatatgat tggtaccaaa acacactttt cgtattgtgt gcagaccata
     9541 caggtgtgcc tttcggtgaa aagtacacca cccgcgaagc cattttccgg ataccgatcg
     9601 tattttattc gcccaatacc agtatcatat ccaaggcaca aatcggtgtg aagcctaatg
     9661 aggcgctcat gtcgcagatt gatattttgc cgtcaatact cgatttattg cattacgacc
     9721 atgattttca ttgctttggt gaaagtattt tcagacatga caaaaagaaa tacacgtatc
     9781 aatacagcaa tggtgttttt cagatattgg atggtgagta tattctgtta tttgacggtt
     9841 cgaatgttgc aggattgtat aattataagc aagacccaat gatgagaaat gacatttcaa
     9901 gtaaagaaac ggaattgacg aagcaaatgt ctgatcaatt aaaaacagtc ctgcagcgat
     9961 acaatagggc aatgatcaga aacgagctga acagataggc tcagtttact ttgtacgcaa
    10021 caataataat gcattatatt tgtttaccat tcaagacata ttacaagaat aaaaatgtta
    10081 ctgaaaaaat acatttttat aaaattggct tgattttggt aatatgaaat ttgtcttaga
    10141 gttacatttc aaaatccgtt ggcagaagcc gacctgtaag aaggattgtt attgcaggta
    10201 tgtgaaacaa atttcccgac attccattcc gattaacatg ttttgacaca taatgatggc
    10261 caaaacataa ccatgaaaca taatgtccat cccagctgaa tcaaaattac gaccgataca
    10321 tcttgccctg tgaaaatagg gcattttttt tgcatttttc tataaaatgt tgcatctttg
    10381 cacgatacta aaaaccgttg gcaagaattg atttttttac acatttagag ctacatgcat
    10441 aaaggtttac ttactgtatt ttttgtgttg ttttctgtat tttttttaat tgctcagtca
    10501 acaaaccaac aaaataattt gtccttggtg ttggacaata ttccgccagt agcggtgtgt
    10561 aagagtcatg tccagattag ccttacagac aatccgacca aggttttccc ggtttctttc
    10621 gatgacggaa gctacgatga agaatgtctc gctcagttgc ttgtcaaacg gatgaatgag
    10681 cctgagtcag ctttcaaacc atttatgtat tttacttgtg acgacatcgc taacagcccg
    10741 gtaaaagttg tgttgcgagc cgtggattgc gaaggtaatg tgaatgactg ctggtctgag
    10801 gctgatgtgg aagataaaac aaagccggta gtagtttgcc cggccgacct tactatcttc
    10861 tgcacacaag acccaaatca atattttaca cagcctacag caatagacaa ttgtgggaca
    10921 ttgacattta ccagtaatgt catcgataac agagaccagt gtcaaaatgg ttatgtttca
    10981 aaaatatgga cagtaaaaga caatgccaag aatcagggta cttgtacgca tcgcgtatat
    11041 tcaaaacacg tttctgatta tactgtgacg ttcccgagtg acgtcaccat caataaatgt
    11101 gtatcgcctg atgatctcac caaaacagga gaaccaatat ttaaagataa acagtgcgat
    11161 ctacttgcag taggccatac ggatcatgac ttgccggtga cagacggcaa tggttgcttc
    11221 gtaaggatta gaaaatggca gattattaac tggtgtacat acgacccgaa taaagccaca
    11281 catacgccac tgggtattcc gataggtaat aaaaaataca aagatgatga cggattcttc
    11341 gaatataccc aatatataaa agtattcgaa accgccgacc ctgtccttac ctgcaaagat
    11401 actacgatat gtttccaaaa tgattgcgaa gtgagctttg acattcctaa gcccgctgtt
    11461 gttgattgta gccagaatat ttattatagc ctttccggtg atttgggtag taaatattca
    11521 gtatccggcg tgaaatcagg agtatatcag attcaacaca atgtatctga cggctgcggc
    11581 aaccatagtt tttgcaatgt taacattaca gtaaaggact gcaaaaagcc aacaccggtg
    11641 gtacatcaaa aacttgccac tacattgatg gttcaaaacg gtttggtcga agtgtgggct
    11701 tcagattttg aaaccagtag ctatgacaac tgcacaaaat cagagaatct gaaattctca
    11761 tttagtccgg acaaaaacga tatttcgaaa attttcaatt gcgacagtct cggtcttcgc
    11821 acagtaaaaa tctgggtaac agacgaagca ggcaatcagg attatgctat tgccaccatc
    11881 gatattcagg ataatatgaa tgcttgcgat actagtggca caatcattca ggtgtccggc
    11941 tcagttaaaa aacctgatgg gagccccatg gtcgatgtac atatcaaaaa tccatacgta
    12001 gaagttataa ccgactcagt tggatatttt gctacaccat tgctcaatat gcatttacct
    12061 tatacgatgg aatgtataaa acaggaagac cctaaagcag gtttatctgt cttggatgca
    12121 atattgctcc gtgaccacat attaggatat aaaaaactta gcaatccgta ccagtacctg
    12181 gcaggcgatg tcgataagtc aaaagggatc aatacttacg acctgttttt attaaagcaa
    12241 atgatactag ggcaccttga agaatggcca aagaatcaaa cttttaacta cataagtaaa
    12301 aagaaaactt ttgataagcc tgaagaaata tggaatgatg caactacagg actgttgctt
    12361 gattcacttg cgaccgattc actcgtgttt gatctggtag ccattaaaac aggagatctc
    12421 agtgatgcag accaacttat tggtagtgat aatatttcta caagggcaag tacaaccatt
    12481 agttttgtaa gtggcaataa aattaattat accggagatg gcccggcaaa gggcatccag
    12541 tttgctgtga atatcaaggc attgtccacc gaaatatatg cagagcaagg aaccatttca
    12601 ataactgatg ttgacaatgg aatgtttgaa gtgaaataca ttacagacga agtggttata
    12661 ccaggccaga ctctattatc atggcataat acggataaaa atttcttgcc ggtattgacc
    12721 aatagccttc aaccaatgtc agtagccgaa aacaacgaac tccaacagct tgacttgaaa
    12781 caccctaagg taaattttcc acttgaggaa ggtatatttg tgtatccgaa tccggcaaaa
    12841 aatgctgtcc gtgtaaagat tacctcagct ggagcacaga tggctaccat cgaagtaatt
    12901 gatcttgaag gcaaagttat agatcacttt gacaagaatc tgattgaagg cgtcaattta
    12961 tatgcattgc ccattaatac aggtacaggc attttcatca ttaaagtaga gacccaacgc
    13021 aatatctttc aggagaaaat cagcatcata gagtaacagc ctaattgctt ttagacttat
    13081 cttcgtgaat gatttcacta gaaggtagag ttcctgtttc gcttaacaaa ttgcagttat
    13141 tgaattatct gcaggcagag gtacgtttgc atcatgtatc gccctatacc tcacgtttac
    13201 tgactatcga atccgagctt cagcattttc agatgttgga ttttaacata agctcaccgg
    13261 caaatcaact gattgtagat atagaaatgt tgataccggt aaatgtccgc ctccctcttt
    13321 gtaaattccg ggtgaacact aaaccaaaga tcaagataag tattttaggt accgggtttt
    13381 ctggcacatc aaagatagaa tccctaagtc tcagcctccg cacattgcaa ctcaacggag
    13441 ccttatcggt ttttgagggc atgatccgga aaaatattat tgcacaagta gaaaaactat
    13501 taccggatat attgaagctt gtaaattcaa aaatttctga gctactatct caaaagactc
    13561 aaattgattt taacatttca ggcatagact acaacaatct cctatatgta aaaggtgtga
    13621 agctacgaga atgcagtagc agcaacaata gcctgctcat tgatgtattc tcatctgctt
    13681 atatagagaa tcaaaatcaa ggattcggag cggagtatta tgtaatcgag cgaaaagagc
    13741 acacgggtca gtttaatgtt gctattcatc aggattttct gaaatcgata ttgaccaaac
    13801 tcattaccga agtcgaaatt cctgtattga ataaaaaaat acctttggta ctgacagatt
    13861 ttcgggtagt gtgtaatgta ctgtatgtag gtttggagct aagcgaattt tacaaaggag
    13921 cctggcggct aaagcttgat accagtgtta cgagtgatac attgagattc tctttgctcg
    13981 aaatgcaatc caccggtagt aattctttgt ttgataaagg tattacaaaa ttgctcgaac
    14041 tcggcatcga gaagctcata caaaaatatg ctgaaattcc cttttcaacc ataaatgctc
    14101 atatagcagg tggtatcaaa gatgcctatc atttcgattt tcagagttcg gccatcaaac
    14161 taagtgttcc gaagttttct gtaaaacata ttacatttga tgacaagatc gtattggtca
    14221 actgcgaatg gcaagatcgg ttctatctgg aattataggc gttttaactg caggatgatt
    14281 tcattaccac tgtcgttcat cctcagaatg agagacgtga gtcgtagctg aatgatttcg
    14341 actgctaccg gttggttggt ttggatattg gaaggctgag tgatgagcat atttttttca
    14401 agtttatatt tgccatgctc cgtatattct gaaggatttg tgtaagaata gtcgccattc
    14461 agcttaaatg taaattttac cttagaccaa tccattttga tactgttgcc attttcagca
    14521 acatgagcac ctacccaatt gcccagaatg atgtatttct taaatccacc aaggtttatc
    14581 aatacaaaaa tcaaagtaat gaatgcaact aagaacagca ggatatttct ttttctaacc
    14641 ataccaataa aaggacaatt gtaattttga gtaaacgaat aaagatgaaa aatattagaa
    14701 attatttgta tattgaatga atttgtcggc gaatcacaga aattcatttg atatttcgaa
    14761 gcagatgaag gcataaactg tacttactta gagcaaatat gttttttctg atgtgtaaat
    14821 ccattaactt tgcggtctta tggtaaagat tagaaatatc gaaatagcag attttccgct
    14881 gatactggcc ccaatggaag acgtaagtga tccgcctttt cggtttcttt gcaaagaaga
    14941 aggggcagac ctcatgtaca ccgagtttat atctgtagaa ggtctcatac gtgatgccga
    15001 taagagtctg agaaaacttg atatttatga aaatgaaagg cccataggta ttcaggtatt
    15061 tggtggtgaa atagatagta tggtagaagc catggccgtg gtcgaggatg cagacccgga
    15121 aataatagat atcaattttg gttgtccggt taatcgtgtt gtttgtaaaa tggcaggagc
    15181 gggtattctg caagatatac cgaaaatgat aaaactaaca gagtcggtcg taaaggcaac
    15241 atctaaaccc gtaaccgtta agacaagact cggctgggat gaaaattcca tcaacataat
    15301 cgaagtagcc gaacgcctgc aggatgtagg tatcgaagcc ctcaccatac atggccgcac
    15361 ccgcaaacaa atgtacaaag gacaagcaga ctggagttat ataggcaaga taaaggaaaa
    15421 tcccaggatg cacataccca tattcggcaa tggagatatt gactctccgg acaaagcaaa
    15481 gatttaccgc gagcgatacg gtgtggacgg aataatgata ggtagggcaa gcattgggta
    15541 tccttggata ttcagggagg tcaaacatta ttttaaaacc ggcgaactac ttgtagcgcc
    15601 tacaatcgca gagcgcgccg atgcagccag cagacattta caacatgcca tcagatggaa
    15661 gggagaaaag cttggtatat tagagaccag gaagcattat accaattatt ttaaaggatt
    15721 tccagacatc aagccattca gggcacgcct ggtcacagct gacaaacctg aagatgtatt
    15781 tgcagtcatt catgaaatga aagataaata ttcggtgaca gaagttttca tgtcataaag
    15841 tttaacaaat caaagacaat tttggaaaat tttcacctat atagcaccga aagggacata
    15901 cttaatgtag tgcaaaaagc agcagagcta ttgaacatac aggcctatct cgtaggtggc
    15961 tttgtacgtg accgcttgct tggcagacct tgtaaagacc tggatatagt gtgcacgggc
    16021 gacggaatat tgctggctga aacagcagca tccatgttgc cggggaaacc caaagtaagt
    16081 gtttttcaaa gatttggtac cgccatgcta cgttttcgtg atatggagat agaatttgta
    16141 ggtgcaagaa aggagtcgta tgtgtctgat tccagaaaac cttacgtcga ggcaggtact
    16201 attgaagatg accagcagag aagagatttt acaatcaatg caatggccat cagcctccag
    16261 aaggaagatt ttggtacttt ggttgatcca ttcaatggcg tgcagcatct ggaagaaaaa
    16321 gtgatacgaa ctccattaga gccaagaaaa acattttctg acgacccgct caggatgcta
    16381 cgagccatca ggttctcggc ccagcttcag tttgagattt ttcccgagac attggcttct
    16441 attacagata attgcgacag gatactaatc atttcacaag agagaatcat agttgagtta
    16501 gaaaaaatac tactgagcat aaaaccatct gtcggcttcg aacagttgta taaaacggga
    16561 ttgttaaaac atattttacc cgaattggta gaacttcagg gtgtagagta cattgatggc
    16621 aaggggcaca aggacaattt ttaccacaca cttgaagtta ttgataacct ccggagcaac
    16681 acggataata tctggttgct atgggctgcc gtatttcatg atattgccaa acctgctaca
    16741 aaaaagttta ctccagatac aggctggaca ttccatggtc acgaatatct gggatctgta
    16801 atgttgccaa gaatatttcg aaaactaaaa ctaccaatgg atggcaaact taaatatgtg
    16861 caaaagctgg taagcatgca cctgagaccc atttcactta caaaagagaa cataacggat
    16921 tcggctatta ggaggctcct ttttgatgcc ggtgatgata ttgacgatct gatgttgttg
    16981 tgcgaagctg acattacgtc taaaaatgct gaaaaaaagg ccagatttct tgaaaactac
    17041 agagttgtaa agcaaaaact catagaaata gaagaaaaag ataacatacg taactggcaa
    17101 cctccaatat caggtgaatt aatcatgtca acattcaata tcaaaccggg tagggaagtt
    17161 ggtctgataa agaatgccat acgtgaggct atacttgacg gtgtcattgc caatgagtac
    17221 gaaacagcct acaagctgat gatggaaaaa ggtgcagaac taggcttggt gattgtgtga
    17281 tgcaaaaaaa gagttccaac atagttgtga aactcttttt aagattttag agggtaaaaa
    17341 tgctttgaca gcggtatttt ttaccttatt gatactagtc aaaaagcttt ttgtaactgt
    17401 cgccgtcttc ttttttgtca ttttcgcgtg ggagcatttc cggatgattg ttaagatcga
    17461 gcgtgtcact tttgccaccc tgcatcagca gcagctggct ttggttttcc cattgttcga
    17521 gcattttcag gccttcctct tcgaatacgc cattctgcag atcaacggaa tccatgaatt
    17581 ttgatgacat atccatgaat ctttccattt cgcctacttt attggcgaca tcgtcagcaa
    17641 tggcttctac agccatatcg aacatcgctt ttttatcttt atcgcccgat ataactttca
    17701 tggctgaaga catagcagaa tggctggctc tgatggcttc acgttcttct tgctttactt
    17761 ttacctgatc tctggtgtcc tccagtaaga tttcactgtt ttcgtacatt ttggcaagaa
    17821 ttctgtgcag aatatccatt tttgaataca gagaattgta tttttcgttc gaatctttaa
    17881 gtcttgcagc ctggcgtgta gagagtacga gctgtttttc gtttccttgt ttttttgcag
    17941 catttgctat attcaactcg ttttcaatac ttttatcatt ctcctccatg gtagtcttta
    18001 gcttgcgcat tgagcctcgt aggttgccta tttgcttact catctttgac aaattattct
    18061 caaggtcttc gacatagctc ttcagaatgc ctataggatc aaggttgaca aatatacctg
    18121 taatccagcg catgatactt ttgtagccgt accagaagag attgcgcatg cgcgggtcaa
    18181 cgaccatata tattacagca gctagggcaa tcaataatag agccagatac aatgtgttgg
    18241 ataccaatgt gataagtatt ggtaacattt tatataacag atagcccaga ccaacgatga
    18301 tgccggtcat aaaaatgccg cctgtgaccc cttcgggtct ttgccagaat gtctttggtt
    18361 tgaaatcaga aggaggtgta attgaagcca tagttgatat tagttttgtt cagaacaaat
    18421 atacaaatgc agtgagatta aagttcgcag acgctcaatt attttaaaat tttatttgca
    18481 aaggcaaaag aaaagctttc ggcaaaactt ttttcagact gtctttgtcc gaacccacaa
    18541 tgtccgcctt tcggtgtaag cgccagtgtc agtatgtctg atgattgtgc aaactgcttc
    18601 ggattacttt ccacgggtat cattgggtca tcaacggagt tcagtagcag ggtaggcaca
    18661 cggatgtctt caagccagtt gttgatggta gcattagcat aaagttcttg tcggctgttg
    18721 tatccgttga aaggcgcgct gtattgttca tcaaattggg cccagctttt gatatttgac
    18781 aaaccggctt tgtcaatgat gcccgggtat tgtttctctt tttcggagag ttttaccata
    18841 aggcttttca tgaatttgtt tttatacaca aaattggctt tgcggtctaa tgtgtgtgca
    18901 catgcctcaa ggtcgaaagg agttgaaatg gctatgttga gtttgatgaa atctgaataa
    18961 agtacagctt tttttgcaac aaatttggta ttaatgacac cgcccatgct ataaccaccc
    19021 aatgctattt gactgtattt atacttgtca agcacatgtt ctattacttc tgttatatcg
    19081 tgtatatcgc catgatggta tagtttcgga gcaatgttca tctcaccgct acagctgcgg
    19141 cagttccagg ctagtatgtc ccagccgttt attgaaaatt cttgtgccat gcctttgacg
    19201 tactgtttgt tggaatttcc ttccaggccg tgtgtgatga tgaggagttt gtcatttgct
    19261 tttttaatcc agtcgaggtc caaaaaatcg ccatccgcca gtgtcaatcg ttcacgtaca
    19321 tactgtatgt tgtctatttt gcgtgtcata gaaggtacga tagtagccag gtgtcggttg
    19381 ttgctgtacc agttgggttt atagttttta ctgagatcaa taataggcat tactttttat
    19441 aaattttgtc tatttttgtt aaaatgaatg gttgaatatg cagatagcca agggtctaat
    19501 aatagccgga gtaatcctta tcattatcgg attgatttat tatttctttc aggacaagct
    19561 cggatggctg ggcaggttac cgggcgattt tagttataaa aaagataatt tcagtttttt
    19621 tgcacctatt accactatgc tgattctgag cattttatta tcagtcatca ttaacttgat
    19681 cagaagattt ctctgaacac tttgtactac ttaaacttta gcaggcactt atttttctta
    19741 ccattttcta taaacaggta tttgccacaa agcaaagcgt ggctgtcaat ttttgcttca
    19801 atagttgaca tcttttgttt gttgatactg atggcattgc ctgttatggc tcgtcgggct
    19861 tcgctcttag aaggcagaat accagaagca tcagaaagca gggtaagtaa gtctgtctga
    19921 tctaaagcag agctactgtt aaattcgtaa cattgtattt cggcacctac ggtctcccat
    19981 tcttcttcag aaattctctt taagtaatct tcgtcgagtt tagggttaaa taacagttcg
    20041 gttactttca gcacagactc aaaatcttga gccgagtgca cacggcatgt gatgtctttg
    20101 cctagctctt tctgcaggat acgtaggtgt gctgcttctt tgtgctgatt ttcgagctct
    20161 tctatttcat ctttagtctt gagggtaaat atacggatgt aagtgcttac atcttcgtca
    20221 gatgcattga gccaaaactg gtaaaacttg taaggagatg ttcgcttagg gtcgagccac
    20281 acattacccg attcagattt tccgaatttt gttccgtctg cttttttaat aagaggtgtg
    20341 gtaattgcat acgcggtacc ttgtgcttta cgtctgatga gttcagtacc ggtgacaata
    20401 ttgccccatt ggtcagagcc acccatctgt aacttgatgt tgtggttttt ccagagataa
    20461 tagaagtcat aaccttgtat gagctgatat gtaaattcgg taaaagacat acctacttcg
    20521 ctatccaggc gtttgcgcac actgtctttg gccatcatgt agttgacggt tatgtgtttg
    20581 ccggtatcgc ggataaagtc gaggaagctt atttccttaa accaatcgta attgttgact
    20641 atttcagccg gattggtttt actttcaaag tcgagaaatt tttcgagctg ttttttctgg
    20701 caggcaaggt tatggttgag cgtatcttgg ttgagcaggt ttctttcttc acttttcccg
    20761 gttgggtcgc ctaccatacc tgtggcgcca cctaccaaag ctatcggtat gtgaccacat
    20821 tgctgcagat gcaataatgt cataatctga accaaattgc caacaccgag cgaatcggct
    20881 gtcgggtcaa atccaatgta cgcctttact acttccttgt tgaggagttc ttctgtgcct
    20941 ggcatgatgt cctgcagcat acctctccat ctcagttctt ctacaaaatt ctttttcatg
    21001 aataaaattt cgatacaaaa ataatgattc tgcagaatat aagtttctta ttttaagact
    21061 gtgatattca gatttctgct taatttcaca acatgaaacg cacaaccttc ctgtcaggtt
    21121 tccttgccgg ccgactttca gcagtacgcg aacgcaattt ttcgtcttta atcgtacgcg
    21181 taggtgtggt ggctacggct atcagtattg ctgtgatgat tattgccact tgccttataa
    21241 gtggattcca gaaaagtata agcgaaaaaa ttttcggatt ttgggggcat atacacatta
    21301 tacataacag tgagctgaac agttacgaga gcaagcccat ttccgttaga cagaatttct
    21361 atcctgagct gaaagacaaa ggccccatca actataccgg gccacaagaa gtactgggtt
    21421 ttgtgtttcc gaactggcag acacagcaca ctaccaaagg gaaaatccgc aacattcagg
    21481 catttgcctt caaagccggg ataatgaaga ccaaatcgga gattgaagga atagtgctga
    21541 aaggcgtaga tgaacatttt aactggacat ttatcaaaga ttttatcatc agaggcaagc
    21601 ctgtagccaa tatcggcaaa ggttcgcgtg atattctcat ctccgaacag acagccgaca
    21661 gacttaaaac agacgtaggc aagtcgctta ttctatattt tgtcaataat ggtgagcaaa
    21721 tcaagaaaaa gtttactgtt tcgggtattt ataagaccgg acttgaggaa tatgaccgga
    21781 aatttgcaat cattgatatt gcaatcatca gagaattaat gggctgggca cctgatgaag
    21841 ttggcggctt tgaagtattt ctggatgatg tagcagacct ggatgcttac aatacctatc
    21901 tgtacgatca ggtagtaccc gatagtcttt atacagaatc aatcaggatg aagcttccga
    21961 atatattcga atggctcgaa ttgcaaaaca ccaatgagca gcttattatc ggacttatga
    22021 tgatagtagc catcattaac ctcagtacag ccttgctcat ccttattgtt gaccgtacca
    22081 atatggtagg tatgctcaag gctttgggtg caagcaattt tttggttcag aaggttttta
    22141 tccggtttgc cgggcatatt ctggtacgtg gtttgttttt tggcaatttg ttcggattgt
    22201 tgattgcagg agctcaaaaa tattttaaac tcatcaagct ctctgaaaag gattattacc
    22261 tgagttatgc accgatatat tttgactgga gtaaaataat gatgattaat gtatttgctt
    22321 tcttggtagt aatcctaatt atgctgatac cttcattgat ggtgaggtca atatcaccac
    22381 tgaaggccat cattatgaaa taattaatac tttacttgta cttaaaagct ataaagttaa
    22441 taataaaaga ttgtaaattt gcagtgcaaa tgaataagtg caactgtctt gtcaccgagt
    22501 agctgtgctg attttttaaa ccctgattac gtgttaggaa cttaaaacga aaataattag
    22561 tacaaaaaaa atccatatac aagtgctaaa gcattgagga caatgaattt aaactattga
    22621 catggatttg aagaaataat tgtgcgtcgt taagagtcta atgcgtgttc cggattaaac
    22681 cttattcttt catcatggaa aataacggag atgatcagat acttcagaaa agaacatgga
    22741 cagttgatgg aactggagaa ttttgctccg ggttgttggg tgcacatctc aacacctttt
    22801 agctccaacg agttagaaga ttttgctgag cgctttgata tagagcttga ttatctgacc
    22861 gactcgcttg accccgaaga acgttcgcgc tacgaaaaag aagaccattt caaatttata
    22921 ctcatcaata caccgatacc caatcaacgg gatgatgaat accagcctgt gtttatgacc
    22981 gttcctatcg gtatcatctt tgccgaagaa ttcatgataa ccatttcggc atttgataat
    23041 accattatgg acttgtttct aaaagataaa gtcagacatt tcaatccggg cgactaccag
    23101 ctgtttattc tacagatatt agaccagaac gttaacaggt ttacgagtta tcttaagaag
    23161 ctgaacctca accgggacgt aatagagcag caactcataa caagtagccg caataacgag
    23221 cttaaacaat tgctaggcct cgagaagagt tccatttact tcgttaacgc actcagttct
    23281 aacgagcttt tgaagctgaa actaaaaagg acggattttc ttggcatcaa cggtgacgaa
    23341 gacaaggtcg atttttttga aaatatcatc attgacaaca atcaggccca ccagatggcc
    23401 aatgtattta cgcatatcat caatggtact acggatgcta tggcctccat tatctctaac
    23461 aacctgaatg tcatcatcca acgatggaca atgataacaa ttgtgctgat ggtacctaca
    23521 ctgatagcta gtttttatgg aatgaatgtg aagcatttgc catttgatga ttcggattac
    23581 gctttctatt atatagtgat tctctcactg gtgcttacct tgcctttagt ttgggtcatt
    23641 aagcgcaagg aacttttcta agcgatattt ttagtaaaat cggaagggag acacttccgt
    23701 ttgagaataa agccgacatc ttattcacag ttttttgatt tatttcaaca aagcaaaaat
    23761 attgattatc aactaattgt aatacttatt aacatagtgt tgataagtaa aatcatggtt
    23821 tgttgactac aatattccat aaaaagcttc ggttgtctag tacacagaaa gtgcgaagct
    23881 tgatctggaa gttgtgggta ttaacagaag ggtaaaggag aagcaaagat ttttattcac
    23941 actgaataat gcctgacaaa aacagttttt tacaacagat gcatattgaa gctattcttc
    24001 cgtattgtta ataattctac tgtacttggt aagcagatta atctaaatct tactgcaact
    24061 ttccggtaaa attgactaat tttgtcacat aataatgcca acaaagccta tatttcattg
    24121 gcaagataag caatctatga taccaacatt aaacacacta ttatgcaaaa gttcaacacc
    24181 attgatgaat atcatgacca gcagtctggt gcaatacagg taatacttca actgttaagg
    24241 caggctatca ggcaggctgc acatcaggca acagaagtga ttagctatgg tatgccggct
    24301 ttcaaacaaa ataaagtgct ggtttattac gctgcaggaa aaaaccatat agggttttac
    24361 ccgacgcctg accctattgt tcatttcaaa aaagagttgg aagggtacaa gacttcaaaa
    24421 ggggcaatac agtttccgat atctgaaaat ctgccgtggg acctgatacg gagaatagtg
    24481 gagttccgtc ttgtaactga tactgcctta gctaaatcaa catcaagtaa gctgcgaaat
    24541 gtaacagaaa agccaacaga tatacctgct tatcatgcaa tacaaaaccc ggtacacaag
    24601 gagatatgtg aaatat
//
LOCUS       DAOQQY010000011        24158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_624_length_24158_cov_5.426669, whole genome shotgun sequence.
ACCESSION   DAOQQY010000011 DAOQQY010000000
VERSION     DAOQQY010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 24158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24158
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_624_length_24158_cov_5.426669"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="PLE23_01350"
     CDS             complement(<1..922)
                     /locus_tag="PLE23_01350"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HMX81940.1"
                     /translation="MNINTFKAIYPNTSFITEPESYFEGVKNEYIELKENGFFNKDGE
                     ELLYIYQIQTADRSFLGLVAAVSVHDFINGHIKGHEETLLAKEQVHVNIVLRNRAFLK
                     PVLLTYPHIHQISKVLETYISTHDALISIEEDIHGQTHTFWQITESKINEQLIEMFNK
                     QVNVAYIADGHHRSSTTALLYNRLVGKPEQQDFSTLLAAFFPSDQLIIEEFNRVVEIP
                     DHITDIAFMARLSKVCDITPLKTPHKPRKKHEMTMYLKREWFVLKWRNRLIKKYLSEG
                     LVLDVNLLNIEILQKILEVEDIKNDPDVRYI"
     gene            complement(990..1490)
                     /locus_tag="PLE23_01355"
     CDS             complement(990..1490)
                     /locus_tag="PLE23_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HMX81941.1"
                     /translation="MDFTEGKEKFLNAWGALASSWGINRTMAQVHALLLVAPQPMCAE
                     SIMEDLKISRGNANMNIRALMDWGLVYKEIKCGERKEFFYAEKDIWEVFRRIVEQRKK
                     KELEPIIKLFDDVTSVQEECDASKEFCKVVRELKVLSCQADSVLNKITNADQSWITKG
                     ILNVFK"
     gene            complement(1553..2662)
                     /gene="gcvT"
                     /locus_tag="PLE23_01360"
     CDS             complement(1553..2662)
                     /gene="gcvT"
                     /locus_tag="PLE23_01360"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMX81942.1"
                     /translation="MKNTPLTDIHINLGAKMAEFAGYNMPISYAGIKEEHLTVRSGVG
                     VFDVSHMGEFILKGKNATRLIQNITTNDVSTLEPGHVQYTCMPNGNGGIVDDLLIYRL
                     PNDGLAEDEQAYLLVVNASNIEKDWNWISQHNHLDVEMINTSDNTGLLAIQGPKAAEA
                     LQSLTDIKLNEVKYYTFLKGTFAGIPDTIISATGYTGAGGFEIYVSNDHLVQLWNTVF
                     EAGKDYGIQPIGLGARDTLRLEMGFCLYGNDIDDTTSPIEAGLGWITKLNKGEFIDCE
                     LYKSQKENGTARKLVGFKLDDRRVPRHGYLITDADGNKIGEVTSGTQSPSLGYPIGMG
                     YVQKEFSKADTPIFIDFNGKRLQAAVTKLPFYKES"
     gene            complement(2666..4684)
                     /locus_tag="PLE23_01365"
     CDS             complement(2666..4684)
                     /locus_tag="PLE23_01365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMX81943.1"
                     /translation="MTKKILNIFTGLAAVFFLYLSACVPPSNIDNTKVNATFRDTVWQ
                     RVYMLQEKQDIRGLVGLFRHKNPTLRYGAVMAFASIQDSTVLDSLYMMLADKNLKVRE
                     AAAFALGQIGSPKATRHLLAAYDNKDSLNQKTILNATILEAIGKCGSADYLKPLTTIS
                     TFYRSDTLLLLGQMRGLYRYGLRGITSHETVKRVVQVIKDKNMPAPVQVMAANYLARV
                     QNIDLDSFHVELLTSLSTNADPYVRMAVASSLSKIKKTDISEQLKNIFRKEKDYRVQV
                     NVLKTLKSATYESSRHFFRTVLNSLNVHVAGEAANYFLEHGIPKDAGWYWSLARDTLK
                     EPLSVKMYAAASKHLQGYFKDAKAALNAELKYRFNQEKDPYIKAAYIQALGQWPFNYT
                     FLRQSGLSSGYIPVRTAALESLVAILKDPAFYYHFGERVKDAKRELVEIMAQAIVSND
                     VGLIAVASQALREPKLNFKLWFPDYSFMTSALHRLKLPKDLEAYLELEKTIAFFSDSP
                     EPQLKKMAYTHQLDWKLLNTIKDKCTAVVETNRGKITIELYPEQAPATVANFIQLAQS
                     GFFNNKYFHRVVPNFVIQTGCPRGDGYGSLNYTIRSELSPTHYDTEGYVGMASAGKDT
                     ECSQWFITHSPTPHLDGRYTIFGKVKEGMDIVHAALQGDIVKKITIQY"
     gene            complement(4681..5061)
                     /locus_tag="PLE23_01370"
     CDS             complement(4681..5061)
                     /locus_tag="PLE23_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81944.1"
                     /translation="MNSFQAHIFNVFVLIVFGLWGALDSYDQAGTSMQYKSLIAPAAG
                     LFLLVLSPWIAKQRNRSMIIAASVILLLLVYFYLEFMKTDGEPRLRIIVQALSNVWAL
                     ILLIKNLFTNKFRKPQPTETELPL"
     gene            complement(5079..7733)
                     /locus_tag="PLE23_01375"
     CDS             complement(5079..7733)
                     /locus_tag="PLE23_01375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMX81945.1"
                     /translation="MAENSNTEFKDIRSLRFKRWGNRLWKVSLYSLLIFVMFLVLLSF
                     DNLPSFTDLEDPTNNLASPVYANKGELIGSYYIENRIPVEFKDLPQHLVDALVSTEDS
                     RFYIHSGIDPEALARVFTKTIIMGEKSSGGGSTITQQLAKLLYSDRDFSGMTKTQKVV
                     GLITRKFKEMITAVKLERRYTKEEILALYLNHFNFINGAYGIKAASQIYFGKIPKELN
                     VAEAATLVGMLQNPSLFNPLRRPEKVKERRNAVLRKMEKAGKLSHTDYKKYSARNIDL
                     SNFSQQNPSEGLAPYFRMELRKEVIRILNRPECRKPDGKPYNIYKDGLKIYTTLDTAI
                     QNCTETAVADHMKSLQKTFFQRWKGKDPWMFIKPKRAEPYTSKKEGEEEVKPRTEEEE
                     RAFRARMLWANVQSSERYGVLRSRILNNLISEIQDEYESIRLSDSDIERMIAYTTDKE
                     VFKNLIKKRYLKKEKAEIYLNILKSDFWPKLRSKYEELQEKARKDFDTPVKMKVFAYN
                     KTFEKDTVMKPIDSIKYHRMFLQTGVVTIEPTTGYVKSWVGGINYKYFQYDHVTSERQ
                     VGSTFKPFIYATAIAMQGFSPCFQVQDVPQTIKVGEGTFRLDRDWTPKNFDYYSGKYY
                     TLKEALKKSINTVSVFLMKQIGDTEPVRGLIHNMGIDSSARRDNGILRIPKEPSICLG
                     TPDLSVLEMTAAYATFANEGKYHKPIIITRIEDKNGKVIFQETPLEKVALSPVSDYVM
                     VEMLKHVASGAGGFGNVHSVFGGKTGTTNDAADGWFMGINPQIVAGIWVGGEDKWVTF
                     LDQNEGQGSFMARPVFAKLLENIEKNKNIDYDINAQFIKPEGEMTIELDCYKYQLSGI
                     ETQSDAKKAEIYEDPFDEGGAPVDDFVE"
     gene            complement(7784..8830)
                     /locus_tag="PLE23_01380"
     CDS             complement(7784..8830)
                     /locus_tag="PLE23_01380"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HMX81946.1"
                     /translation="MSHNTAVIWQNWHHLNNIMEGNYSKIILLTDTVVAKHCLGYFRE
                     KFRRNIDHVITIEAGEENKSMMSAASIWQELNLCKADRKSLLICLGGGMICDLGGFAA
                     SVYKRGIKTMSVPPTLLAMTDASIGGKTAIDFEGIKNLIGSFHLPDSIFIDTHFLATL
                     PAAEWLNGYAEIIKHAIIADAELWNLLNPEHFRPTIISDDLLTRSINIKQSIVAVDPF
                     EQTERKKLNFGHTAGHALEAYFFNKEKTLSHGQAVAAGMIIESYINMCSGKLSEKTYE
                     QIRDFILKYFPAIGFKNEEIADVAELCKHDKKREFGTLTITKIIAIGLSEPDLIITEQ
                     QLKEGLEAYIRATS"
     gene            complement(8846..9145)
                     /locus_tag="PLE23_01385"
     CDS             complement(8846..9145)
                     /locus_tag="PLE23_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HMX81947.1"
                     /translation="MSNPVKRIVKMQFKVEETANFINIFLESKPLIRAFPGCNHVELL
                     RHSEDTTIMFTLSHWESEENLEQYRQSELFKSTWAKTKVLFREKAQAWTLEEFKP"
     gene            complement(9142..9915)
                     /locus_tag="PLE23_01390"
     CDS             complement(9142..9915)
                     /locus_tag="PLE23_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HMX81948.1"
                     /translation="MDKKNILTLTTDLGIRDYYTAILKGEALKTAPDISIIDITHQIM
                     DYNLSRAAFIFKNAYYHFPENTIHFVAVNSDRTDCPQFLVFRHRNHYFIGPDNGFFSL
                     VFDEDPDLIFPVHYDKADKFPLKKVLQTGLKTILEEKFSALGNPISKYQKKLAFHPVT
                     GSALIRGSVIHIDNFDNVVVNIHHELFEYIRQGRNYEIYYKRWDPITSISEHYQDVPV
                     GEVLARFNSSEYLEIAVNMGRASTLLGLETDDSIQIDFL"
     gene            10048..11073
                     /locus_tag="PLE23_01395"
     CDS             10048..11073
                     /locus_tag="PLE23_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMX81949.1"
                     /translation="MSNSEIVLHIEEVEPMELYGEQNIKLTLLKNAFPDVTFTSRGSQ
                     LKLSGSKKETQNAKAKVEMMIRILKSHKELSTQTVEDLLAGENPFETKIAPTDGSAAL
                     VYGREGKVIKAKTKNQKLLVESADANDVVFAIGPAGTGKTYTAVALAVRALRNKTVKK
                     IILTRPAVEAGESLGFLPGDMKEKVDPYLRPLYDALDDMIPLEKLNYFMTNRIIEIAP
                     LAFMRGRTLDNSFIILDEAQNATTVQLKMFLTRLGPSAKCIITGDLTQIDLPHHQKSG
                     LRLSLNLLKDIPGVGIVELSTEDVVRHRIVKAIIKAYDSYHEQQKQIKELEKHKESPQ
                     NDIKTTE"
     gene            11070..11483
                     /locus_tag="PLE23_01400"
     CDS             11070..11483
                     /locus_tag="PLE23_01400"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMX81950.1"
                     /translation="MNTAEILYKGGLRAESIHLRSGNVIVTDAPVDNHGKGEAFSPTD
                     LAATSLGICMVTVMGILADAHNIDIDGTRITIMKHMGSDPRRISGIDVDLYMPDRDYS
                     DKEKKMLEHTAYNCPVAKSLHPDIVQNVKFHWKNL"
     gene            11480..12721
                     /locus_tag="PLE23_01405"
     CDS             11480..12721
                     /locus_tag="PLE23_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMX81951.1"
                     /translation="MSVILPHLTKALVGQIDTGGSKSISNRLLIMYALAGRDAESARL
                     SSADDTLVLKRLITQKSAIYDCGKAGTALRFLLALKAYQNQRCIITGDNRMKERPIGI
                     LVDVLNEMGACINYTEKEGFPPVEIQPGLGLAGSVIHVDATVSSQFLSALALIGPYLS
                     GGLTLVLKGKMVSSPYFDMTLKMMQHSGIHIERSDGRILIHPGKYSFTAFRVESDWSS
                     ASYFFAFCALLPGSQIELYGLMPDSLQGDSMIVRWMEKFGVQTDFTENGIRIRSEKII
                     LPTIVEFDFIDNPDLFQTIAFMCAVLGVQVLYKGLDTLKDKETDRIAAVSAELAKVGV
                     HLVKLSPRMAKKTNTQYYLQEGKANFDKIRINTYADHRMVMAASLLSVTGEVEIEHPQ
                     LVKKSYPDFFNHLKLLTRPDK"
     gene            complement(12740..13381)
                     /locus_tag="PLE23_01410"
     CDS             complement(12740..13381)
                     /locus_tag="PLE23_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81952.1"
                     /translation="MKYINLTLLLLLFIACAQKENTGDTGNKTEKPVEEKLDFFAEND
                     SSAWYKKYPMLRESTTDTPFDSLSLQQKFQMLESRAFFKDRKYEPGAFGDKYYAFLRD
                     TSRNTFEKDLVCTHIKFKPGTAELVGLDTKELNDIIMASYSSLNLYFRVNVNATDAAL
                     RQARLESVKNAFTQARVNLAKIIFDDSLSGKTSSDELVLHLFQHKPGDSVKKK"
     gene            13657..17010
                     /locus_tag="PLE23_01415"
     CDS             13657..17010
                     /locus_tag="PLE23_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMX81953.1"
                     /translation="MRYFRVIFYVASILLVANGCKQKEGSTQAKDSLFVSMAGTETGI
                     DFKNAITENAETEDNILSYPYFFHGGGVAVGDINNDGLADVFFTANQGPNKLYLNQGN
                     LKFKDISATAGIGKKNWSTGVTMADVNNDGFLDIYVVQGGNMNNPETRSNLLFINNGN
                     LTFTEKATEYGLDDKGIGTQASFFDMDKDGDLDCYVMNESKYEGVVLHQVFQDLKNHQ
                     NLLNASGKLYRNDGNKYLDITEQAGVVRYGYGLGLVTTDLNNDGWTDIYVANDYSVPD
                     FMFINNKNGTFTDMTKQMTRQIEFYGMGVDVNDINNDGLPDIGVVDMATDDHFMGKTL
                     MTSMDVKGFWYYIDSLKYQYQYMFNALQLNNGNNTFSNIANLAHVAKSEWSWASLFFD
                     ADNDGFKDYFISNGFKRYARDNDFRNEMERIRKENGGSVPMSMRKEMYEKMPSFKLSN
                     YMGKNNGHIAFDNVAEEWGLAEPSWSNGAAYGDLDNDGDLDLLVNNIDDEAFVYKNTA
                     ADRKENNFLNISLKHDKPTENTKVYAVAGNDIYSQELCPTRGYCSAMDRKLHFGLGKL
                     NRVDALYVQWPDGKTQKLSNVKANQLLELKYTDASNTPLPKFHESAGIVEKLNPESVG
                     LDFVHKENPYNDFATEVLLPHKQSTIGPKLTKGDANGDGLEDLYIGGAANQSGVLYLQ
                     TAAGKLVKSPSQPWDADKACEDMDAVFFDIDGDGDNDLYVVSGGGGEMANYPRTLQDR
                     LYINEGKGQFVKANGAVPEDFFAGSRVKAADFDSDGDIDLFVAGRAVAGKYPYPDRSM
                     LLMNDKGKLKDVTAAWSKDLMNPGIVTDFIWTDFNKDKKPDLVLVGEWMGIRFYENKG
                     NSFVDVSTAKGTADTHGWWYSIAEADVDNDGDMDLIAGNVGLNTKFKAKPEKPFTVYS
                     ADFDGNGTNDIVLTKDYKGKVVPVRGRQCSSEQMPFIKERFKTYKDFANADIGDILGD
                     DKLDKALKLTATEFASVLFINENGKYSQKNLNIMAQIAPVNAIITQDLNEDGNTDIII
                     SGNNFDTEVETQRYDAGTGLILLGDGKGNFEPLPAYYTGFYADGNTKDMILFTLGSAK
                     TPYLLLANNNSAPELFRLNNKKIQNVAYH"
     gene            17036..20404
                     /locus_tag="PLE23_01420"
     CDS             17036..20404
                     /locus_tag="PLE23_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMX81954.1"
                     /translation="MKAYLKVICILSFIIMSLSSCKNEQRSNSTTKDGAAHVPKFQEL
                     SPTETGLSFSNNLDIEQLRPIFEYINGFDGGGVACGDFNNDGLTDVFFTGNFVDNKMY
                     FNKGGMKFEDVSEKAGIVLKGSWCSGVATADVNNDGFLDIYVCRNYYDSPQLRENVLF
                     INKGDGTFTDQAKQYGINDNAYTITATFLDYNKDGLVDLMTGNHHPNRTRLFMDNYKD
                     FLNPPHEYSTHLYKNNGNGTFSDVTKEANVQYFGWVLGLSACDFNNDGYTDIFVAVDH
                     DEPDALFINDGNGKFTNQIYTAMYHISMSSMGVDFSDINHDGFQDLVVAEMAAMSNFS
                     EKANMASMDIQRFWNLTKLGYHYQYMRNMLHLNNGNNTFSEIGQLAGIDRTEWSWSPL
                     FFDADNDGEQDLFISNGYYKAIQFKDKYKPFNKKMSHEKDEKKKVEMVKEFLRSVNPI
                     RVENQFFRNNGDLTFSQLSKESGIANRATISTGAAYADFDNDGDIDLIVCNLDEPVSL
                     YKNLEQENGNNNHYLRVKFEPSKKMHPHGAKVVVETETGSQYREFNTVRGFQSAVEPV
                     IHFGIGKNKTVKKLRVEWPDGKCQIMENIKADQLISLDYEKAAPNGSCIPKPELFDLA
                     QNFTKESGVSFKHTENSYDDYQTQILLPHNMSQWGPHISKADINGDKLEDFYIGGAAG
                     QEGTLYIQNADMTFRKTSQPAIAADKKYEDIGSVFFDADGDGDTDLYIVSGGYEFGPE
                     GTMYQDRLYLNDGKGNFTKSKDLPAITSSGSCAIPFDMDGDGDLDLFRGGRQVGSEYP
                     KPAKSYIMRNDKGKFIDVTSDILPDNGVLGMVTDGAVADLNEDGRQDLLITGEWMPLT
                     FLINEGGKFVNRTKEMGFDGSNGWWFSIEPGDFNNDGKTDFVLGNLGTNYKYKATKEK
                     PFDIYGNDFDENGKYDIVLGYLLDGKETVPVRGRQCSSQQIPQIEQKFKTYTDFANAN
                     LDEVYGKDNLDKSIHYRVTSFKSGILMNKGSGKFEIRYLPNEVQMAPIMGIQILDINN
                     DKIQDIIVGGNLFVSEVETGRADAGKGRILIGDGKGGFKPVSVLQSGLYIPNDVRDIK
                     LVNTSSPDVLLLLVGNNNYYMDVIKINKKKIPAI"
     gene            20532..23438
                     /locus_tag="PLE23_01425"
     CDS             20532..23438
                     /locus_tag="PLE23_01425"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMX81955.1"
                     /translation="MGEYSGYKLVLSIDNHDVIGITVSAWAAPSPDMREVSTVSQKIF
                     LNNYRDFFPDTIESHHKLFQVLDEISDESLTARFGAKIRNGDKLSVLMGDEKIKAVLK
                     HFIEKKIDFILRQLVENDIVLCINIFRKAYIADHCVDVSSTSSMANISLKKSDTSVSY
                     KLNLISDEQDVALMDKNIMVVCNHPAWAIIDRKLYQPVGINGNMLLPFATKNVVIVPD
                     KLVHTYFKSFVLKLDSSVEIEAEGFDIDKYDIPENPELQLTESVFEGKFVLKLACYYH
                     EQKFEFHDIRQGRKKIEISPEGNIRILNYVRNMETERHIAEKLTECGLEKTAGQVFAL
                     PEQSVADMLDACLWLRKNKPALVAAGFKVAPFFMGENPVNLEAFDVKTVAKEAGDWFD
                     VHVVVNVGEFSFPFKKLAANIRNEDPVFMLPDGSVFIIPKAWFEKYSSLVQLGTETES
                     GYRLSKAQATVAEELTGVAARLSEQNEAEIPLPEGLNAVLRPYQEAGFRWLARLYDNN
                     LGACLADDMGLGKTLQSIALLLYISSKKSKIQTTGLPSGQLDLFAAPQRTGIRALVVL
                     PASLVFNWYIELEKFAPQLSKYTHTGTERYRSHSLIGGFDLVLTTYHILVRDIDELSK
                     IAFDAIILDESQQIKNKDSKMFEAVNRISAPFRLTLTGTPIENSLADLWAQMQFINPG
                     MLGSAQSFKQAFRIPIERNQDEAKREELLRIVKPFILRRTKKSVTPELPELTEFVHYC
                     EMDEQQAEIYEKEKSAVRNSILMAETDGSGKEKLRILNALQRLRQIASHPAMLPEFAD
                     VASGKFDEVSYFLENAVQCKEKVLVFSSYVRHLDKYCDWATSNKVAFSRLTGDVALAQ
                     RQREVENFQEKDIPWFFLSLKAGGTGLNLTAAELVFLIDPWWNPAAEQQAIARAHRIG
                     QNRNVTAIRFITRNTIEEKILRLQQKKQKLASEIITEDEFFFSLDKSELAGLLE"
     gene            23862..>24158
                     /locus_tag="PLE23_01430"
     CDS             23862..>24158
                     /locus_tag="PLE23_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81956.1"
                     /translation="MIKLLSVSASKAEVPPMPATLLTDCTDTTYINIHTDSLSNVLSL
                     LPPTGPVNMKISGTLTVDVNWYIAPGSDIILDDISGIDVKPGVFLSADSTLFRGC"
BASE COUNT         7018 a         4885 c         5219 g         7036 t
ORIGIN      
        1 ctatgtacct tacgtccgga tcgtttttaa tgtcttcaac ttcaagaatc ttttggagaa
       61 tctcgatgtt taagagattg acatcgagta cgagcccttc tgaaagatat ttttttatta
      121 acctgttgcg ccatttcaac acaaaccatt cccgcttcaa atacatagtc atttcatgtt
      181 ttttacgtgg tttatgtggt gttttgagtg gcgttatatc acacacctta gacaatctgg
      241 ccataaaggc gatatcggtt atatgatcag gaatttctac tacccggtta aattcttcga
      301 tgatcagctg gtcagaaggg aagaaagcag caagcaaagt actgaagtct tgttgttcgg
      361 gtttgcctac caggcgattg tacaataatg cagtcgtaga agagcgatgg tggccatccg
      421 caatatatgc tacatttact tgtttgttga acatttctat caactgttca ttaatctttg
      481 attctgtgat ctgccaaaac gtatgcgttt ggccgtgtat atcctcttct atacttatta
      541 gcgcatcgtg tgttgaaata tatgtctcga gtactttaga tatttgatga atgtgtggat
      601 aggtgagtaa cactggcttt agaaatgcgc ggtttcggag tactatgttt acgtgtactt
      661 gttctttggc aaggagtgtt tcttcatgac cttttatatg gccgttgata aaatcatgaa
      721 cagataccgc tgcaaccaaa ccgaggaaag aacggtcagc tgtttgtatc tggtaaatgt
      781 acaacaattc ttctccgtcc ttgttaaaaa agccattttc cttgagttcg atgtattcat
      841 tttttacacc ttcaaagtaa gactccggct cagtgataaa gctggtattc ggataaatag
      901 ctttgaatgt gttgatattc atatacgtac aattaaattc ctgatattct aagccgatta
      961 ttcaaggaca ttcaggagta tgcagtttat tacttaaata cgtttaatat tcctttagtt
     1021 atccaagatt ggtcggcatt tgttatttta tttaatacag aatcagcctg acaagacaat
     1081 acttttaatt cacgaactac tttacaaaat tcctttgatg catcgcattc ttcctgcacc
     1141 gaagtgacat catcaaacaa tttgataata ggttccagtt cttttttctt tcgttgttca
     1201 acaatccggc ggaagacttc ccaaatgtct ttttcagcat aaaagaattc ttttctctcg
     1261 ccgcatttta tttctttgta cacaagaccc cagtccatca aagcacgtat attcatattg
     1321 gcattgcctc ttgaaatctt aagatcttcc atgatagatt cagcacacat cggttgcgga
     1381 gcaactaata gcagtgcatg aacttgtgcc atagttcggt ttattcccca gctagaggcg
     1441 agtgcgcccc aggcattcag aaatttttcc tttccttccg taaaatccat ttattatgta
     1501 cttacttaac ttcctaatta aagaaagaaa tggcaaaatg gttcaatttg acttaacttt
     1561 ctttgtaaaa aggtagttta gtaacggccg cttgcaatct tttgccatta aagtcaatga
     1621 aaattggagt atcggctttg ctaaattctt tctgaacata acccatacca atcggataac
     1681 cgagtgacgg tgactgtgta cctgaagtca cttctcctat tttattgccg tcagcatctg
     1741 ttatcaggta accatggcgc ggtactcttc tgtcatcaag tttgaaccct acgagtttgc
     1801 gtgccgtacc attctctttt tgtgacttat acaattcgca gtctataaat tcgcctttgt
     1861 ttagtttggt aatccagcca agtccggctt caataggaga tgttgtgtca tctatgtcgt
     1921 tgccgtacaa gcagaatccc atttctagtc tcaaggtatc tctggcgccc aagcctatcg
     1981 gctgaatacc ataatctttt ccggcttcaa atactgtgtt ccatagttga acaagatgat
     2041 cattggatac gtaaatttcg aagccacctg cgcccgtgta tcctgttgca ctaatgatgg
     2101 tgtcgggtat gcctgcaaat gtgcctttta ggaatgtata atattttact tcgttcagct
     2161 taatgtctgt aagactttga agtgcctcgg cagcttttgg accttgtatg gccagcaatc
     2221 ctgtattgtc tgatgtattg atcatttcaa catcaagatg attgtgctga gaaatccagt
     2281 tccaatcttt ttcgatgtta gatgcattta ctacaagaag gtaggcctgt tcatcttcag
     2341 ccaatccgtc atttggcaaa cggtagatga gcaaatcatc cactattcct ccattgccat
     2401 ttggcataca agtatattgt acgtgacccg gctcgagtgt tgagacatcg ttggttgtaa
     2461 tattttggat aaggcgggtt gcatttttac ctttgagtat aaattcgccc atgtgcgaaa
     2521 catcaaatac accaacgccg gatcttactg taaggtgttc ttcttttatc cctgcataag
     2581 atatgggcat attgtagcct gcaaattcgg ccatttttgc tcctaagttg atgtgaatgt
     2641 cggtaagtgg tgtgtttttc atattttaat attgaattgt tatttttttg acaatatcgc
     2701 cctgcaaagc agcatgaaca atatccatgc cttcctttac tttgccgaat atagtatatc
     2761 tgccatcaag atgcggagta ggagagtgtg tgataaacca ttgtgagcat tctgtatctt
     2821 tgcctgctga tgccataccc acatagcctt cggtgtcata atgtgtcggc gataattctg
     2881 accgtatcgt ataattgagt gaaccgtagc catctccccg cggacatcct gtttgtataa
     2941 caaaattagg aaccacgcgg tggaaatatt tattattaaa aaaaccggat tgtgcaagtt
     3001 ggataaagtt ggcaacagtg gcaggtgctt gctcagggta aagctcaata gtaatttttc
     3061 ctctgttggt ttctacaacg gcggtacatt tatctttgat agtgttcaat aatttccagt
     3121 ctagttggtg ggtgtaagcc atttttttaa gctgtggttc gggtgaatcg gagaaaaagg
     3181 ctattgtttt ttcgagttcg aggtatgctt ccaggtcttt tggtagctta agtctatgca
     3241 gggcactggt cataaatgaa taatccggga accacagctt gaagttgagt tttggttctc
     3301 tgagtgcctg tgaggccact gctattaggc ctacatcatt gctaactatt gcctgtgcca
     3361 ttatttcgac aagctcgcgt ttggcatctt tgacacgttc gccaaaatgg taataaaatg
     3421 ccggatcctt taatatagct accagcgatt cgagtgctgc tgttcgtacc ggtatgtacc
     3481 ccgatgacag acccgactgt cttaaaaaag tataattgaa aggccattga cccaatgcct
     3541 gtatgtatgc cgccttaatg tatggatctt tctcttggtt aaatctgtat ttcaattcag
     3601 cattcagtgc agctttggca tctttgaaat agccttgaag gtgtttactg gctgcagcat
     3661 acatttttac tgatagtggt tctttaagtg tatcgcgggc aaggctccag taccatccag
     3721 catctttcgg aataccatgt tctaagaaat aatttgctgc ttctcctgct acgtgtacat
     3781 tcaggctatt gagtaccgtg cggaagaaat gccgcgaaga ttcgtatgtc gctgacttaa
     3841 gtgtcttcag cacatttacc tgtacgcgat aatctttctc ctttcggaaa atgtttttta
     3901 attgctctga aatatccgtt tttttaattt ttgacaatga gcttgccacg gccattctaa
     3961 cgtaaggatc agcattcgtg ctaagtgaag taagtagttc aacatgaaag ctgtcaaggt
     4021 caatattctg aacacgtgcc agatagtttg cagccataac ctgtacgggt gccggcatat
     4081 ttttgtcttt aatcacctgg acaacacgct taacggtttc atgcgatgtg ataccacgta
     4141 gcccatacct gtacaatccg cgcatctgac caagaagtaa aagtgtatca ctcctgtaaa
     4201 atgtggaaat agtagtaagt ggtttcaaat aatcggcaga gccacatttg ccaattgcct
     4261 ccaagatggt ggcattaagt atggtttttt ggttgagtga atctttattg tcataagctg
     4321 ccagcagatg tcttgttgcc tttggtgaac ctatctgacc cagtgcaaat gcagcggcct
     4381 ctcttacttt caggtttttg tcggcaagca tcatgtacag actatccagt acagtcgaat
     4441 cttgaatgga ggcgaaggcc attaccgcac cataacgaag cgtcggattc ttgtgtctga
     4501 acaaaccaac tagtccgcgg atgtcttgtt tttcctgaag catatacaca cgttgccata
     4561 cagtgtctct gaaggtagcg ttcaccttag tattgtcaat gttggacggg ggcacacaag
     4621 ctgacagata cagaaagaat actgctgcca gccctgtaaa tatattcagg attttcttag
     4681 tcataacggc agttctgttt cggtgggttg tggttttctg aatttgttgg taaacaggtt
     4741 cttaataagc agaatcaatg cccatacatt tgacaatgcc tgcactatta ttctcaatct
     4801 tggctcacca tctgttttca taaattcaag gtaaaagtac acaagtaaca gcaggatgac
     4861 tgatgcagca ataatcattg aacggtttct ttgcttggct atccacggtg acaagaccag
     4921 cagaaataaa ccggctgccg gagcaataag gcttttgtac tgcatggatg tgccagcctg
     4981 gtcataagaa tcaagagcgc cccacagacc gaagactatg agtacaaata cgttgaatat
     5041 atgtgcctga aatgaattca ttccactcat tattttaatt attctacaaa atcatctacc
     5101 ggagcaccgc cctcgtcaaa tggatcttca taaatttcag cctttttggc atcactttgt
     5161 gtttcaatac ctgatagttg atatttataa caatcgagct cgatggtcat ttcaccttcg
     5221 ggttttatga actgtgcatt gatgtcgtag tcaatatttt tatttttttc aatattttcc
     5281 agcaatttgg caaaaacagg ccttgccata aaagacccct gaccttcatt ttggtcaagg
     5341 aaagtgaccc atttgtcttc accacctacc cagatacccg ccacaatctg aggattaatg
     5401 cccataaacc acccatcagc agcatcgttg gtggtaccgg tttttccacc aaacaccgaa
     5461 tgcacattcc caaatccgcc ggctccggag gctacgtgtt ttaacatttc gaccatcaca
     5521 taatccgata ccggactcag tgctaccttc tcaagtggtg tttcctggaa gatcactttg
     5581 ccgttcttat cttctattct agtaatgatg attggcttgt ggtatttgcc ttcattggca
     5641 aatgtagcgt aggctgccgt catttcaagt acagaaaggt caggagtgcc aaggcaaatg
     5701 gacggttcct tgggtatgcg gaggatacca ttgtctctgc gtgctgagct gtcgataccc
     5761 atattgtgta taaggcctct taccggttcg gtgtcaccta tttgtttcat cagaaaaacc
     5821 gatacagtat tgatcgattt ttttaatgct tcttttaaag tgtaatattt gccggaataa
     5881 taatcgaagt tttttggtgt ccagtctcgg tcaagccgga atgtgccctc gccaaccttg
     5941 atggtctgcg gtacatcctg cacctggaag catgggctga acccctgcat ggcaatagct
     6001 gttgcataaa tgaatggttt gaaagtagag ccgacctgcc tctcactggt gacgtggtcg
     6061 tactgaaaat acttgtaatt aatgccacct acccatgatt ttacgtatcc agtagtaggt
     6121 tcgatggtga caacaccggt ctgcaagaac attctgtggt atttgataga gtcaatcggc
     6181 ttcatgacag tatctttttc gaatgttttg ttgtaggcga acaccttcat ttttacaggc
     6241 gtatcaaaat cttttcttgc cttttcttgc aattcttcat atttgctacg cagcttgggc
     6301 caaaaatctg atttcagtat attcaaatat atttcagctt tttctttctt caagtatctt
     6361 ttcttaatca gatttttaaa tacttctttg tctgttgtat aggctatcat ccgttcaatg
     6421 tccgaatcgc ttaacctgat agattcgtac tcatcttgta tttctgatat caggttgttg
     6481 agtatccttg accttagcac accataacgc tccgaactct gtacatttgc ccagagcatc
     6541 ctggctctga atgcacgttc ttcttcttct gttcttggtt ttacttcttc ttcgccttcc
     6601 ttcttgcttg tgtaaggttc tgcccgtttc ggtttaatga acatccaagg gtctttgccc
     6661 ttccaccgct ggaagaatgt tttttgcagt gacttcatgt ggtcggctac tgcggtttct
     6721 gtacagtttt gtattgcagt atcaagggta gtatatattt taagtccgtc tttgtatatg
     6781 ttgtaaggtt tgccgtccgg ttttctgcat tcgggtcggt tgagtattct tattacttct
     6841 ttgcgcagct ccattctgaa ataaggtgcc aggccttctg atggattttg ctgactgaaa
     6901 ttggacaggt cgatgtttct ggcactatat tttttataat cagtatgaga aagtttgccg
     6961 gctttttcca ttttgcgcaa cacagcattt cgtctttcct tcactttttc gggtcgcctc
     7021 agcggattaa acaatgacgg attttgaagc atacctacca atgtagctgc ttctgctacg
     7081 ttgagttctt ttggtatttt accaaaatat atttgtgatg cggcctttat tccataggct
     7141 ccgttgatga agttgaagtg gttgaggtaa agtgccaata tttcttcctt ggtatatcta
     7201 cgctcgagct tcactgccgt tatcatttct ttgaattttc gggttatcag ccctacgact
     7261 ttctgcgttt tggtcattcc cgaaaaatcc ctgtcagagt acaagagctt ggccaactgc
     7321 tgggtaatag tactgccacc gcccgagctt ttttcaccca tgataattgt ctttgtaaat
     7381 accctggcaa gcgcttcggg gtcaatgccc gaatgaatgt aaaagcggct gtcttcagta
     7441 gaaactagag catcgacgag gtgctggggt agatccttga attctaccgg aatgcggttt
     7501 tctatgtagt acgaaccgat cagttcgcct ttattggcat ataccggcga tgcaagattg
     7561 ttggtcggat cttcgagatc tgtgaaactt ggcagattgt caaatgataa tagaacgaga
     7621 aacataacga aaattaatag actgtacaag cttactttcc acaaacggtt cccccatctt
     7681 ttgaatctta ggcttcgaat gtccttaaat tctgtattag aattttctgc cattttaata
     7741 atcagtaatt tgtagcaaat atatgaatta actgttgcaa tatttatgat gtagcacgaa
     7801 tatatgcttc aagaccttct tttaactgct gttcagttat tatcagatcg ggttcgctca
     7861 ggccgatggc aataattttg gtaatggtca atgtgccgaa ttcgcgtttt ttgtcatgct
     7921 tgcatagctc tgccacatct gctatttctt catttttgaa gccaatggcc ggaaaatatt
     7981 ttaggataaa atctctgatt tgttcataag ttttttcact caacttgccc gaacacatat
     8041 tgatgtacga ttcgataatc atgccggctg ccacagcctg accatgtgaa agtgtttttt
     8101 ctttgttaaa aaaatatgcc tccagtgcat gacctgccgt gtgaccgaag ttgagtttct
     8161 tcctttccgt ttgctcaaat ggatcaactg caactataga ttgctttatg tttatagatc
     8221 tggttaaaag gtcgtccgaa attatggttg gtctaaaatg ctcagggtta agtaaattcc
     8281 ataactctgc atcggcaatt atggcgtgtt tgatgatttc agcatagccg ttgagccatt
     8341 cggcagcagg taatgtggca agaaaatgtg tatcgataaa aatactgtca ggcaaatgga
     8401 aactgccaat gagattcttg attccttcga aatctatggc ggtctttccg cctatacttg
     8461 catccgtcat agctagtaaa gtgggaggta cggacatggt ctttatcccg cgtttatata
     8521 ccgatgctgc gaatccaccc aggtcacaaa tcattcctcc gccgaggcag atcaacagac
     8581 tttttctgtc tgctttacac aagttaagtt cttgccagat ggatgcagca ctcatcatcg
     8641 atttgttctc ttcccctgct tcaatggtta tgacgtggtc aatgtttcgc ctgaattttt
     8701 ctctaaaata tcctagacaa tgcttagcta caactgtatc cgtcagcaat ataatttttg
     8761 agtaattgcc ctccatgata ttgttcaggt ggtgccagtt ctgccagata acagccgtat
     8821 tatgcgacat ctgatacaat ggtcgttagg gtttaaattc ttcgagtgtc caggcttggg
     8881 ctttttctct gaacaatact tttgtctttg cccaagtact tttgaatagt tccgactggc
     8941 ggtactgctc cagattttct tcgctctccc aatggcttag ggtaaacatt atagtagtgt
     9001 cttctgaatg tcgaagcagc tcaacatggt tgcatcccgg aaaagcgcgt atcaacggct
     9061 tactttccag aaaaatattg ataaaattag cagtttcttc gaccttgaac tgcatcttta
     9121 cgattcgttt taccggattg ctcatagaaa atcgatttgt atgctgtcat ctgtttcaag
     9181 tccgagcaaa gtagatgctc tgcccatatt tacggctatc tcgagatact ccgatgaatt
     9241 aaatctcgcc agcacttcac ctaccggtac gtcctgatag tgttcagaaa tgctggttat
     9301 cgggtcccat cttttgtaat aaatctcata gtttcgaccc tgacgaatgt attcaaacag
     9361 ttcgtggtga atgttgacca ccacattgtc gaaattgtct atgtgaatga ccgatccacg
     9421 gattaaagca cttccggtca ccgggtggaa ggccagtttt ttctgatatt tactaattgg
     9481 gttgccaaga gctgaaaatt tttcttcaag tatggttttt agcccggttt gcagaacttt
     9541 tttaagtgga aatttgtctg ccttgtcata atggacggga aaaataagat ccggatcttc
     9601 atcgaataca agtgaaaaaa aaccattatc gggcccgatg aaataatgat ttctatgcct
     9661 gaaaaccagg aactgtgggc aatcggttcg gtcagaattg actgccacaa aatggatagt
     9721 attttcagga aaatgataat acgcattctt gaaaataaaa gcagctcgac tcagattgta
     9781 atccattatc tggtgtgtga tgtctataat actgatgtca ggtgccgttt tcaaggcctc
     9841 acccttgaga atggcagtat aataatctct gataccgaga tctgtagtaa gtgtaaggat
     9901 gtttttttta tccaaaatgt attgaatcta aatgtgaatt aaaaataatt tctgcaaaat
     9961 taaataaaac cattaagttt ggattgtttg atgtttacag aaattgtaaa ttgtagccga
    10021 atattaattt ttaacaaaaa tcagaacgtg tcgaatagcg aaattgtatt gcacattgaa
    10081 gaagtcgagc caatggagct ctacggagaa caaaatatta aacttacttt attaaagaat
    10141 gcattcccgg atgtcacctt tacatcgcgt ggaagtcagc ttaaattaag tggcagtaaa
    10201 aaggaaactc agaatgccaa agccaaggta gaaatgatga taaggatact gaagtcgcac
    10261 aaagagttgt caacacagac agtggaagat ttgctggcag gcgaaaatcc tttcgaaacc
    10321 aaaatagcac ctaccgatgg gtcagcagct ttggtctatg gtcgtgaagg aaaagtaata
    10381 aaagctaaga ccaaaaatca gaaactactc gtagaaagtg ccgatgccaa tgacgtggtg
    10441 ttcgccattg ggcctgccgg taccggcaaa acctatactg ctgtagcgct ggctgtacgt
    10501 gcgctgcgta acaaaaccgt aaaaaaaatt atcctgacaa gacctgctgt ggaagccgga
    10561 gaaagtctgg gttttcttcc gggcgacatg aaagaaaaag tggatcccta cctgcgccct
    10621 ctgtatgacg cacttgatga tatgatacca ctcgaaaagc tcaactattt catgaccaac
    10681 agaatcattg aaatagcacc acttgctttc atgcgtgggc gtacactcga taattcattt
    10741 attatactcg atgaagctca gaatgccact actgtacaac tcaaaatgtt cctgacgcgt
    10801 ctggggcctt ctgccaaatg catcattaca ggtgacctca cccagataga cctccctcat
    10861 caccaaaagt cgggccttcg tctgtcgttg aacctactga aagatattcc gggtgtaggc
    10921 attgtagaac tgtcaaccga agatgtggtc aggcacagga tagtgaaggc tatcatcaag
    10981 gcttatgaca gctatcacga acagcaaaaa cagattaaag aattagaaaa acataaagaa
    11041 tcacctcaaa acgatataaa aacaacagaa tgaacacagc agaaatactc tacaaaggcg
    11101 gactgagagc agaatccatc cacctccgct ccggcaacgt aatcgtcacc gatgctcctg
    11161 ttgataatca cggtaaagga gaagcatttt cgcctacaga tctggcagca acatctttgg
    11221 gcatctgtat ggtgacagta atgggcatac tggctgacgc acacaatatc gacatcgacg
    11281 gtacccgcat taccatcatg aagcacatgg gttcagaccc acgaaggatt tcaggtattg
    11341 atgtagatct gtacatgccc gaccgagatt attccgataa agaaaaaaag atgctcgaac
    11401 atacagcata taattgtccg gtagcaaaat ctctccaccc ggatatagtg cagaatgtga
    11461 agtttcattg gaagaatcta tgagtgtaat actgccccac ctgacgaaag cattagtagg
    11521 gcaaatcgat accggaggct ccaaaagcat cagcaaccgg ttgctcatca tgtacgcttt
    11581 ggcaggtcgt gatgccgaat cagcacgttt atcttctgcc gatgatacac ttgtgcttaa
    11641 acgattaatt acacaaaaat cagccatata cgattgtggt aaagccggta cagcactaag
    11701 gtttttactt gcccttaaag cttaccaaaa ccagagatgc attatcaccg gcgataatcg
    11761 tatgaaggaa agaccgatag gcatacttgt ggatgtgtta aatgaaatgg gcgcatgtat
    11821 taactatacc gaaaaagaag gattcccacc ggttgagata cagccaggct tgggtttggc
    11881 cggtagtgta atacatgtag atgccaccgt cagtagccag tttttaagtg cgttggcctt
    11941 aatcgggcct tatctgtcag gtggacttac acttgtactg aaaggaaaaa tggtttcatc
    12001 cccttatttc gatatgactt tgaagatgat gcaacattcc ggcattcaca tagaaagaag
    12061 tgatggcaga atacttattc atcccgggaa atattccttc acagcattcc gggtagaatc
    12121 tgattggtcg tcagcgtctt acttttttgc tttttgtgcg ttgttgccgg gttcgcagat
    12181 tgaactgtat gggttgatgc cggattcact gcaaggagat tcaatgattg tcaggtggat
    12241 ggaaaaattt ggcgtacaaa ccgattttac agaaaatggg attcgcatac gttcagaaaa
    12301 aatcatactt ccaacgattg ttgaatttga ttttatcgat aaccccgacc tattccagac
    12361 tatagccttt atgtgtgctg tattgggcgt acaagtgttg tacaaaggcc tggatacact
    12421 gaaagataaa gagacagacc gcattgcagc cgtcagtgcc gaacttgcaa aagtcggtgt
    12481 tcacttggtg aaactttcac caaggatggc caaaaagacc aatactcaat attatttgca
    12541 ggaaggtaaa gctaactttg acaagattcg tatcaacaca tacgccgacc accggatggt
    12601 tatggcggcc tcattgctga gcgtgacagg tgaagtagaa atcgagcatc cacaactggt
    12661 aaaaaaatcg tatcccgatt ttttcaatca tctcaaacta ttaacccgac cggataaata
    12721 aaatttcaag ctaatcctac tatttctttt tcacagaatc accaggttta tgctggaaca
    12781 aatgtaatac aagctcgtca cttgatgttt tgcccgaaag actgtcatca aatattattt
    12841 ttgccaaatt aactcttgcc tgagtgaagg cattcttaac actttcgagt cttgcctgac
    12901 gcagtgcagc atctgtcgca ttaacattta ccctgaaata aagattgagc gaactgtagg
    12961 atgccataat gatatcgttc agttcttttg tgtcaagacc cactagctca gcagtgccgg
    13021 gtttgaattt tatatgtgta cataccaggt ctttttcaaa agtgtttctc gatgtgtcac
    13081 gcagaaatgc ataatatttg tctccaaacg ctccgggctc atattttctg tctttgaaga
    13141 aggctcttga ttcgagcatt tgaaactttt gctgaagact taatgaatcg aaaggagtat
    13201 cggtagtact ttctctaagc ataggatact ttttgtacca tgctgacgaa tcattttctg
    13261 cgaaaaaatc tagtttttct tcaaccggtt tttctgtctt attgcctgta tcgccggtat
    13321 tttccttctg ggcgcatgcg atgaacagca gcaataataa ggtcagattg atgtacttca
    13381 ttcttgtttt ttttattttt caaacgagaa aaagctttgc ttgttgcaat caatgtatgc
    13441 agtaaataaa aatcagattc aaatgcgaat cattcgcttg aataaagcgt tcataaaaag
    13501 gagtgtgtct gaccgattta tattttattg tgatcgaatg ttagccaata tttgattttg
    13561 aatacccact tttacccaag gcagggtata atgagacgcc gcaaaacaca actaatttgt
    13621 tttacttacg taatttcaaa gataaatata tcatatatga gatattttcg agtaattttt
    13681 tatgttgcgt ccattttgtt ggtggcaaat gggtgtaagc agaaggaagg cagtacacag
    13741 gccaaggatt ctttgtttgt gagtatggct ggtacagaaa caggcattga ttttaaaaat
    13801 gcaataacag aaaatgcaga aaccgaagac aatatccttt cctatcctta cttctttcat
    13861 ggaggtggag tagcagtagg tgacatcaat aatgatggtt tggcagatgt attttttaca
    13921 gcaaaccaag gtcccaataa gctatatctt aaccagggca atttgaaatt caaagatatt
    13981 tcggctactg ccggaatagg aaagaaaaac tggtcaaccg gtgtaacaat ggctgatgtc
    14041 aacaacgacg gttttctcga tatctatgta gtgcagggag gcaatatgaa taatccggaa
    14101 acacggtcta acctgttatt catcaataat ggcaatctta catttaccga aaaggcgaca
    14161 gaatacgggc tcgacgacaa aggtatcggc acccaggctt cattctttga tatggacaag
    14221 gatggcgact tggactgcta cgtcatgaat gagagcaaat atgaaggcgt tgtactccac
    14281 caggtatttc aggatctgaa aaatcatcag aatttgctga atgccagtgg aaaattgtat
    14341 cgcaatgatg gaaataaata ccttgacatc actgaacagg cgggtgtggt tcggtacgga
    14401 tacggacttg ggcttgtaac taccgacctt aataatgatg gttggacaga catatacgta
    14461 gcaaatgatt attcggtacc ggactttatg tttatcaaca acaaaaacgg cacttttacc
    14521 gacatgacca agcagatgac cagacagatt gagttctatg gcatgggtgt agatgtgaac
    14581 gatatcaaca atgacggatt gcccgatatt ggtgtagtag atatggccac agacgatcat
    14641 tttatgggaa aaacactgat gacttccatg gatgtaaaag gtttctggta ttatatagac
    14701 agcctgaagt accaatatca gtacatgttc aatgctttac aactgaataa cggcaacaac
    14761 acattcagca atattgccaa tctggcacac gtagctaaaa gtgaatggag ctgggcatca
    14821 ttgttctttg atgcggataa cgacggattc aaggattatt ttatttccaa tggcttcaaa
    14881 aggtatgcac gcgataacga cttccgaaat gaaatggaac gaatacgcaa agagaatggt
    14941 ggctcagtac ctatgtctat gcgaaaggaa atgtacgaaa aaatgccttc gtttaagctt
    15001 agcaattata tgggcaaaaa caatggccat atagcttttg ataatgtagc agaagaatgg
    15061 ggccttgcag aaccaagttg gtcaaatggt gctgcctatg gcgatctcga caacgacggc
    15121 gacctcgatc tgcttgtcaa caatatagat gatgaggcat ttgtttataa aaatacagca
    15181 gcagacagaa aggaaaacaa tttcctaaac atttcactaa agcatgacaa gcctactgaa
    15241 aacacaaaag tttatgcagt agcaggtaat gacatctaca gtcaagaact ctgccctaca
    15301 cgcggatatt gttctgcaat ggacagaaaa ctccactttg gcttaggcaa gctcaataga
    15361 gtggatgcct tatatgttca atggccggat ggaaagactc aaaaactttc aaatgtgaaa
    15421 gcaaatcagc tgcttgagtt gaaatacaca gatgcaagca atacaccgct tcctaagttt
    15481 cacgagtcag cgggtattgt cgaaaagctc aatcctgagt ctgttggtct tgattttgta
    15541 cacaaggaaa atccttacaa cgactttgcc actgaagtgt tgttgccgca taagcaatcg
    15601 accatcgggc caaagctcac aaaaggcgat gccaatggag acggactcga agacctgtat
    15661 ataggtggtg ctgccaatca gtcgggtgta ctttatttgc agacagcagc cggcaagttg
    15721 gttaaatctc cttcacaacc atgggatgca gacaaagcct gcgaagatat ggatgcagta
    15781 ttctttgaca tcgatggaga tggtgacaat gatctgtatg ttgtaagtgg tggtggtggt
    15841 gaaatggcca attatccaag aacattacag gaccggctct atataaatga aggcaaaggc
    15901 cagtttgtaa aggcgaatgg tgcagtacct gaagacttct ttgccggttc gcgtgtgaag
    15961 gcagctgact ttgacagtga cggtgatatt gacttgttcg tcgcaggcag agctgttgcc
    16021 ggaaagtatc cgtaccctga ccgctcgatg ctgttgatga atgataaagg gaaattgaaa
    16081 gatgtaactg ctgcttggtc taaagatctg atgaatccgg gtattgtcac agactttatc
    16141 tggacagatt tcaacaaaga caaaaagcct gatcttgtac tcgtcggaga atggatgggc
    16201 atccgctttt atgaaaataa aggaaattca tttgttgacg tatcaactgc aaaaggaacg
    16261 gccgacacgc atgggtggtg gtacagtata gcagaggcgg atgtggacaa cgatggtgat
    16321 atggacttga tagcaggcaa cgttgggttg aatacgaaat tcaaggcaaa gccagaaaaa
    16381 ccatttacag tttattcagc agattttgac ggaaatggta ccaatgatat agtacttacc
    16441 aaagattaca aaggtaaggt ggtgcctgta aggggcagac agtgttcgtc tgagcaaatg
    16501 cctttcataa aagagcgatt taaaacctac aaggattttg caaatgcaga tattggcgac
    16561 atattaggtg atgacaaact cgacaaagca ctcaaactta ctgcgactga gtttgcttcc
    16621 gttttgttta tcaatgaaaa tggcaaatat tcccaaaaaa acctaaacat catggcgcag
    16681 attgctccgg ttaatgcaat cattacacaa gacctcaacg aagacggtaa tacggatatc
    16741 atcataagtg gcaataattt tgacaccgaa gtagaaaccc aaagatacga tgcaggtaca
    16801 ggccttattc tgcttggtga tggtaaggga aattttgaac cattgccggc atattacacc
    16861 ggattctacg ctgatggtaa taccaaagat atgattttat ttacactggg tagtgccaaa
    16921 actccttatc tgctacttgc taacaacaat tcggcaccag aattattcag gctgaataat
    16981 aagaaaattc aaaatgtagc atatcattaa ttaaatgaac cataaacaag ccaagatgaa
    17041 agcttatttg aaagtaattt gtatattatc atttatcata atgtctttgt catcatgtaa
    17101 aaatgagcag aggtcaaatt cgacaacaaa ggacggtgca gcacacgttc caaaatttca
    17161 agagctttca cccacagaga ccggcttgag ttttagcaat aatctggaca tcgagcaact
    17221 cagaccaata tttgaataca tcaatggctt tgacggtgga ggtgtagcct gtggtgactt
    17281 taataatgac ggactcaccg atgttttctt taccggaaat tttgttgaca ataagatgta
    17341 tttcaataaa ggcgggatga aattcgaaga tgtctcggag aaggccggca tagtgctaaa
    17401 gggcagctgg tgttcgggtg tggctactgc cgatgtcaac aacgatggtt ttctcgacat
    17461 atacgtttgt cgaaattatt atgacagccc acaactgaga gaaaacgtgt tattcatcaa
    17521 caaaggcgat ggtaccttca cagaccaggc caaacaatat ggcataaacg ataacgcata
    17581 taccataact gccacattcc ttgactacaa caaagacgga ctagttgacc tgatgacagg
    17641 taaccaccat cccaacagaa cccgtttgtt tatggataac tacaaggatt ttctgaatcc
    17701 tccacatgaa tacagtacac atttgtacaa aaataacggt aatggcactt tcagtgacgt
    17761 gaccaaagag gcaaatgtac agtattttgg ctgggtactt ggtctgagtg catgcgactt
    17821 caataacgat ggctatacag atatatttgt agcagttgac cacgacgagc cggatgcttt
    17881 gttcatcaat gatggcaatg gcaaattcac taaccagata tatactgcca tgtatcacat
    17941 ctctatgtca agtatgggtg ttgacttcag cgatatcaat cacgacgggt ttcaggacct
    18001 ggtagtagca gaaatggcag caatgagcaa ttttagcgaa aaagccaata tggcgagtat
    18061 ggatatccag cgattctgga accttaccaa acttggttac cattaccagt atatgcgcaa
    18121 tatgcttcac ctcaacaacg gaaacaatac attctcagaa attggtcaat tggctggtat
    18181 cgatcggacc gaatggagct ggtcgcctct gttcttcgat gctgacaacg atggtgagca
    18241 ggacctcttc attagcaacg gctattacaa agccatccag tttaaagaca aatacaagcc
    18301 ttttaacaaa aaaatgtcgc acgaaaagga cgagaagaaa aaagtagaga tggtaaagga
    18361 attcctgcgt tctgtaaatc caatcagggt tgaaaatcag tttttcagaa acaacggaga
    18421 ccttactttt tcgcagttat caaaagaaag tggcattgcc aaccgagcta ccatcagtac
    18481 aggtgctgcg tatgccgatt ttgacaacga cggcgacatt gacctgatag tctgcaacct
    18541 cgacgagccc gtatcgttgt acaaaaacct tgaacaggaa aacggaaaca ataatcatta
    18601 cctgcgtgtg aaattcgaac caagcaaaaa aatgcaccca cacggagcaa aggttgtagt
    18661 agaaaccgaa actggctctc agtaccgcga attcaatacg gtgcgtggtt ttcagtcagc
    18721 tgttgaaccg gttattcatt ttggtatcgg caagaataaa actgtaaaaa agttacgtgt
    18781 cgagtggcct gacggcaaat gtcagattat ggaaaatata aaggcagacc aactcatttc
    18841 attagattat gaaaaggccg caccaaatgg tagctgtatc ccgaaacctg aactgtttga
    18901 ccttgctcaa aattttacaa aagagtcggg cgtcagtttc aaacataccg aaaattcgta
    18961 tgatgattat cagactcaga ttttattacc tcataatatg tcgcaatggg ggccgcacat
    19021 ctcaaaagca gatatcaacg gtgacaagct tgaagatttc tacataggtg gtgctgcagg
    19081 tcaggaaggt acactttaca ttcagaatgc cgatatgaca ttcagaaaaa catcgcagcc
    19141 ggctatagct gccgacaaaa aatatgaaga cataggctca gtattctttg atgcagatgg
    19201 tgacggtgat acagatctgt acatcgtaag cggcggctac gagtttggtc ctgaaggtac
    19261 aatgtatcaa gacaggctgt acctgaacga cggaaaaggc aattttacta aatcaaaaga
    19321 cctcccggcc attacatcaa gtggctcatg tgccatacca tttgatatgg atggcgacgg
    19381 tgacctcgat cttttcagag gaggccgaca ggtaggttca gagtatccaa aaccggcaaa
    19441 aagttatatt atgcgcaacg ataaaggaaa attcattgac gtaacatccg acatacttcc
    19501 tgacaatggt gtactcggca tggttacaga tggtgctgta gccgatctca acgaagacgg
    19561 ccgccaggat ctgcttataa ccggtgaatg gatgccactt actttcctga tcaacgaagg
    19621 tggaaaattt gttaaccgaa ctaaggaaat gggctttgat ggctctaacg gatggtggtt
    19681 tagcattgag cccggcgact tcaacaacga tgggaaaact gactttgtac tcggcaactt
    19741 aggaactaac tataagtata aagctactaa agaaaaaccg tttgatatct atggcaatga
    19801 cttcgatgaa aatggtaaat atgacatcgt tctgggttat ttgctggatg gtaaggagac
    19861 agtacctgtg cgagggcgtc aatgttcttc acaacagata cctcagatag agcagaaatt
    19921 caaaacctat accgattttg ccaatgccaa tctggacgag gtctatggca aggataatct
    19981 cgacaaatca atccattacc gcgtcacaag cttcaaatcg ggcatactga tgaacaaagg
    20041 cagcggcaaa ttcgaaatta ggtatctgcc taatgaagta cagatggctc ctatcatggg
    20101 catccagata ctcgatatca ataacgataa aatccaggat atcatagtag gcggtaacct
    20161 gttcgtgtct gaggtagaaa ccggccgtgc tgatgcaggt aaaggacgaa tactgattgg
    20221 tgatggcaaa ggtggattta aacctgtaag tgtcctacaa tcaggccttt atatacctaa
    20281 cgatgtacgc gatatcaaac tcgtcaatac atcctcaccc gatgtgttgt tgctgcttgt
    20341 aggcaataat aattactaca tggatgtaat aaaaatcaac aagaaaaaaa taccagccat
    20401 ttgaccatac tgcatggtca aataaatcta atcctcgatt gcctgccttg atcgtcgggg
    20461 attttgtttt tgtgtaaaaa tgatgtaaaa cgcagtagaa attcagatat tcgccttctt
    20521 ttttgatgaa aatgggagaa tattctggat acaaactggt gttaagtatt gataatcatg
    20581 atgttatcgg tataactgta tctgcatggg ctgcaccttc gcctgatatg cgtgaagtga
    20641 gcacagtgtc gcagaaaatt ttcctcaaca attaccgcga tttttttccg gatacaattg
    20701 aaagccatca taagctgttt caggttttgg acgaaataag tgacgaatct cttactgcaa
    20761 gattcggggc aaaaatccgg aacggagaca agctttctgt actgatgggg gatgaaaaaa
    20821 taaaggcggt tctcaagcat ttcatcgaaa aaaagattga tttcatactt agacaattgg
    20881 ttgaaaacga catcgtcttg tgcattaaca tttttcgtaa agcatatatt gccgaccact
    20941 gcgtggatgt ttcttcgact tcatcaatgg caaatatttc cctaaaaaag tctgatacat
    21001 cagtctctta taagctgaac ctgatttccg atgagcagga tgtcgctttg atggataaaa
    21061 atatcatggt cgtatgcaat catccggcct gggccatcat tgacaggaaa ctgtatcaac
    21121 cggtaggcat taatgggaat atgttgctac cttttgcaac gaaaaacgta gtgattgtgc
    21181 ccgacaagct ggtacacact tattttaagt cgttcgtgtt gaagctagat tcatcagtag
    21241 agattgaggc agaagggttt gacatagaca aatacgacat acccgaaaat ccggaattgc
    21301 agttgacgga aagcgttttt gaaggaaagt tcgtcttgaa actcgcctgt tattatcatg
    21361 aacaaaaatt cgaatttcac gacataaggc agggaaggaa gaaaatcgag atttcgccgg
    21421 aagggaacat ccgtatcctg aattatgtgc ggaatatgga aactgaacgt catattgccg
    21481 aaaaactgac agaatgtgga ctcgaaaaaa cagccggaca agtttttgca ctgccggaac
    21541 aatcggtagc agacatgtta gatgcctgcc tatggctgag aaaaaacaaa ccggcccttg
    21601 tagctgcagg atttaaagtc gctccgtttt ttatgggaga aaatccggta aatctggagg
    21661 ctttcgatgt caaaactgtt gctaaagaag caggcgactg gttcgacgtc catgtcgtgg
    21721 tcaatgttgg cgaattcagc tttccgttca aaaaactcgc agccaacata aggaacgaag
    21781 acccggtttt tatgcttccg gacgggtcgg tgttcatcat tccaaaggcg tggtttgaga
    21841 aatactcgtc actcgtacag cttggcacag aaacggaaag cggctacagg ctttcaaaag
    21901 cacaggcgac ggtcgccgag gaacttaccg gagtggcagc ccgtttgtcg gaacagaatg
    21961 aagcggaaat tccattgccg gaaggtttga atgccgtact tcgcccttac caggaagctg
    22021 gattcagatg gttagcacgg ctttatgaca acaatctggg tgcttgcctt gcagatgaca
    22081 tgggcctcgg caagacactc cagtcgattg ccttgttgct gtatatttca tccaaaaaat
    22141 ccaaaataca gactacagga ttaccttcag gccagctcga tttgtttgca gcaccacagc
    22201 gtacaggtat ccgcgctttg gtcgttttgc cggcatcgct tgtatttaat tggtacattg
    22261 agctggagaa atttgcaccg caactcagca aatatacgca cacaggcact gaacgctacc
    22321 gttcgcacag cctgatcggc ggattcgacc ttgtgcttac tacttaccat attctagtac
    22381 gcgatattga tgaactgtcg aagatagcat tcgatgccat catccttgac gaaagccagc
    22441 agataaagaa caaagacagt aagatgtttg aagcggtcaa caggatatcc gcaccttttc
    22501 gccttaccct gacaggaacg cctattgaaa acagcctcgc agacctatgg gcacaaatgc
    22561 aatttattaa ccccggtatg ctcggtagtg cacaaagctt taaacaagcg ttccggattc
    22621 cgatagagcg aaatcaggat gaagcaaaac gggaggaact cctgcgtatc gtaaaaccgt
    22681 ttatactcag gcgtacaaaa aaatcggtga cacctgagtt gcccgaactc acagagtttg
    22741 ttcattattg cgaaatggat gagcaacagg ccgaaatcta tgaaaaagaa aaatcggctg
    22801 tacgcaattc gatcctgatg gcggaaacgg atggcagcgg aaaggaaaag ttacgaatac
    22861 tcaatgcctt acaacgattg cgccaaatag cgagccatcc ggctatgttg cctgagtttg
    22921 cagatgtagc ttccgggaaa ttcgatgagg tgagctattt tcttgaaaat gctgtacagt
    22981 gtaaggaaaa agtgctggta ttctcctcgt atgtgcgcca cctcgacaag tattgtgatt
    23041 gggcgacatc caataaagtg gcgttcagca gactcacagg tgatgtggcg ttggcacaaa
    23101 gacagcgcga agtggagaat tttcaggaaa aagacattcc gtggtttttt ctgtcgctca
    23161 aagcaggcgg tacaggcctc aaccttactg cagccgagct ggtattcctg atagacccgt
    23221 ggtggaatcc ggctgccgaa caacaagcca tagcaagggc gcaccgcata ggccagaacc
    23281 ggaacgtgac tgccatacgc ttcatcactc gtaacaccat cgaggaaaaa attctccgct
    23341 tgcagcagaa aaagcaaaag ctcgcctcag aaatcattac cgaagacgag tttttcttca
    23401 gccttgacaa gagcgaattg gcaggtttgc tggagtaaat gaggagtctt ttctgatatt
    23461 ttcagataga atcttttgat tagtggtacg aatttgaaaa ccaaaagtat aatctgtacc
    23521 aatttttgcc tgcaaagtat cggccacagt cagatggaga agtttgtgcg ggagtgatgt
    23581 taatattgta caaaacgcaa tatttttggt gaaatgcttg actttttaaa taaaatagca
    23641 tatatttgga gcaaatctta tttactaagc aaaaaaccat agcattatga aagcactgca
    23701 gaatattcgt gattgtttcg cctctaaaca aaattatatt aattttaaac gggggggggg
    23761 taaaatttta ttcgtttagc caagcagtaa ttgttttgat tgtcatggct gcgagttgta
    23821 ttggtattgg cagattgaat tctttgaatc cttttgatac agtgataaag ttgctgtcgg
    23881 tatcagcttc taaggcagaa gtgccgccaa tgccggcaac attgcttacg gattgcacag
    23941 atacgaccta cataaatatt catacagata gtttaagtaa tgtactttcg ttactaccac
    24001 cgactggtcc ggttaatatg aaaatatcgg gaacgcttac agttgatgtc aattggtata
    24061 tagctcccgg atcagatata atattggatg atattagtgg aatagacgtt aaaccgggag
    24121 tctttttgtc tgctgacagc actttgttca gaggctgt
//
LOCUS       DAOQQY010000012        23619 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_641_length_23619_cov_5.451027, whole genome shotgun sequence.
ACCESSION   DAOQQY010000012 DAOQQY010000000
VERSION     DAOQQY010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23619
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_641_length_23619_cov_5.451027"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..1005
                     /locus_tag="PLE23_01435"
     CDS             346..1005
                     /locus_tag="PLE23_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX81957.1"
                     /translation="MFLLAENIKKSYGTVNVLKGIDVNIEKAEFVTIIGKSGAGKSTL
                     LHILGTLDKADEGKLIIFGEDVTRYSSDKLADFRNRHIGFVFQFHHLLPEFTAYENVI
                     IPAMIARRRETDYRIKAEQLLDYLGLTDRMGHKPGELSGGEQQRVAIARALINDPSII
                     MADEPTGNLDSTTSAEIHELIARLRKELGVTFIIVTHNIELTELSDRSLKMSDGLIIK
                     T"
     gene            1011..1730
                     /locus_tag="PLE23_01440"
     CDS             1011..1730
                     /locus_tag="PLE23_01440"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HMX81958.1"
                     /translation="MITYSCKIACAPIRLEQSDKSEMISQLLFGETFKIIKTTKSWKR
                     IKTHDGYEGWIDPRVIANTKAVIKEKCYVNKLWAHVISSGMEMFVPYGSELNPEKDEF
                     SIHDFTFVTKSGPEKIIQDALLFLNTPYLWGGRSSFGVDCSGLMQVLAKVNGYTLARD
                     ASQQVNSGKAVEYGKHNIGDLAFFQDESGKIVHVGILSAKDEIIHASGWVRSDLFDEK
                     GILVESSSLYSHHLACIRRIF"
     gene            complement(1772..2632)
                     /locus_tag="PLE23_01445"
     CDS             complement(1772..2632)
                     /locus_tag="PLE23_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81959.1"
                     /translation="MMLIADGGSTKSNWCLVKGDGLPNDYFNTEGYNPYFADTQSIIK
                     SLESSLPGELNKYQIKHIYYYGAGCSTPEKNRIVEIAMQNVFKNAKVFVGHDLLASAR
                     ALLGNTTGFAAILGTGTNTCLYNGKNITLNIDSLGYFLGDEGSGSYIGRKLLRDYMRR
                     FLPAELHKTFLDTFGLSNEDILDALYNKPLPNRFLASFAKFVNDNRHLDYCSNIVHEG
                     FTSFFENLVSKYPEYRSYSFNCVGSVGFNFADILSSVAEKYGMSTGKIIRSPIDDLVQ
                     FHLDFPELLD"
     gene            complement(2663..4312)
                     /locus_tag="PLE23_01450"
     CDS             complement(2663..4312)
                     /locus_tag="PLE23_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81960.1"
                     /translation="METGKHVKTQSGALTTLITVFFFWGFIAASNGVFIPFCKHYFHL
                     DQFQSQLIDFAFYGAYYLGALGLFFFGTMGGKDLVGKWGYKKSIIYGLLFSLLGAIAM
                     ILAINFGGFTGILVALFIVALGFSLQQTAAQPVAIELGDPATGSQRINLGGGINSFGT
                     MIGPIIVSYALFGAAVYSDSEIQSLGLGTVTILYAFVGGLFLLCALLFIFSKKVPSGI
                     IDEKLEKADKATRTLLIITALLILCFIPVFKSYVGVNTDISEDARHLLETGRLKWLLL
                     ALAVVVFGLLGSWQSAKKNQAGWGAMKYPQLVLGMLAIFLYVGVEVSIQSNLGELLKL
                     PEFGGKQASETSPYISMYWGSLMIGRWVGAIPVFSPGKSLKNLLLVVVPIVAFGLIIL
                     VNSIAQKDMSALYWYIICVFIQMVAFYISQDKPALMLLLFSVLGVVAMLVGIFSTGTF
                     AIYAFLSGGLFCSIMWPNIFSLSLAGLNKYTTQGSAFLIMMILGGGIIPPIQGKLADI
                     SSIGIHQSYWVTVVCFGYLAFFAFIVKRILKNQGIDYDAQV"
     gene            complement(4630..4714)
                     /locus_tag="PLE23_01455"
     tRNA            complement(4630..4714)
                     /locus_tag="PLE23_01455"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4678..4680),aa:Ser,seq:tga)
     gene            5208..8627
                     /locus_tag="PLE23_01460"
     CDS             5208..8627
                     /locus_tag="PLE23_01460"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX81961.1"
                     /translation="MKRNLLAFFMFVLALGFSNRTMAVLPDGSDAKDWTLTDINGQTY
                     HLFDLLNQGKTVFIDFSATWCGPCWNYHNSGALENLYESYGPPGSNEVMVFFIEADLN
                     TNLACLYGLTGCVGGTQGNWVAGTPYPIIDLVPSTGYVASDYSISYYPTIYAICPQTK
                     KVYESGQVPASVHYSYVSSCALDFVVDNVVDAKCNGSKNGSISISTQQGSAPFSYKWS
                     NGAKTQDIVNLAAGSYYVTITDKNGIFVESQEIIVGEPDAVEVQATVVDEGCEGTATG
                     EITADGSGGNPGYTYSWSNGKNTATITGLTQGDYTVIVTDANGCTATNTYSVSAQPLP
                     EAYAGPDDLLNCVHNTLILEGEADFGSEFTYEWSTSNGHIVSGGNTLNPEIDKPGTYK
                     LLVTNTSTGCAREDQAVITSDYTTTQTDAGDDGLVNCTSPSIVLDGSKSSKGANIQYL
                     WTTGNGQIVSGGNTNKATVNKGGTYKLTTTHAVSGCTSEDEVVVTENLTGTTAVLAPA
                     GEINCENPEITLDGTASSQGADVEYSWTTNGGNFTSSTDTNFVKVNAAGEYFLIVTNK
                     NSLCKDTANVVVVSDGEVPVANAGSGSELNCNVTTIQLDGTKSSQGADFDVNWSTQDG
                     NILSGADTYSPTVNAPGTYVIAITNTVTNCTSYSSVVITEDDLTKVPDAIYNYTANLL
                     NLNFQDASTGVPSTYAWDFGDGQTSTEQNPTHTYLLEGTYTICLTVTNACGTDQSCQT
                     ITVSSSLNLPTISDLKITDVTCKGGSNGCIDITVINGIPPYTFAWSNGLTTEDPCNIP
                     AGSYAVTITDTDGNQTVSASISINELYYINVNNTFVTEPDCNDNNGSIALDINSNGGQ
                     LTYAWSHDSTLNNAVAENLPEGNYSVIITDANGCTSQQSVVVTDNGQHLDGSASSLLC
                     WGDQNGQIQLTVSGGTQPYTFQWNNGSTTEDLANLSSGTYTCNVTDSLGCVKTIQLVV
                     SSPDQITADMATVKPQKGKDNGSITATVAGGTAPFQYLWNNGSTDPVITNLAPGTYTL
                     KVIDANGCIRDFDVVLEEAVGTLSLDILEQVNVYPNPSNGNFWIEATFSKNTVVDLTI
                     LDLLGREVVNQQFEGNEFKSQIDMHNFNSGTYFIRISADNGMHVEKMSIVK"
     gene            complement(8769..9824)
                     /locus_tag="PLE23_01465"
     CDS             complement(8769..9824)
                     /locus_tag="PLE23_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HMX81962.1"
                     /translation="MTYLRPTFKHPDLPKNSLGYTFKDYEGGLSTLCAGCGHDSISAA
                     IVEACFRQNIAPHKVAKLSGIGCSSKTPTYFLGNSHGFNSVHGRMPSVATGASLVNQD
                     LMYFGVSGDGDSASIGMGQFVHAVRRNINMVYIVMNNGCYGLTKGQDSATADVGSKSK
                     AGSVNIFQPIDFAGLALELGAGFVAQSFSGDKTQLIPLIEAAMNYKGFAFINVISPCV
                     TFNNNVGSTKAYDYVRQHIDATGTIDFVPTHNEIVADYKEGTQTNIKMHDGSYLQFNK
                     LDTDWNPLERDSSFAALQKARNSQEILTGLIYFEQNPTDLNTMLNTTNEKPLHQFSAN
                     ELSPGSAKLAEVNDDFR"
     gene            complement(9831..11660)
                     /locus_tag="PLE23_01470"
     CDS             complement(9831..11660)
                     /locus_tag="PLE23_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HMX81963.1"
                     /translation="MASSVIVNDFVVKFANVNGTGSASANYLFAKAIFRMGIPVTAKN
                     IFPSNIQGLPTWYEVRVSDKAYYGRREGIDVMVCVNPQSMMQDVKSVKTGGYFIYDNT
                     KKLHPSFVREDINYIGIPLMEMCNREYADPRQRQLFKNIIYVGALTELLHIEFNVYED
                     LFADQFKGKEKLIAPNIQALQLGINYIKENFNHTFPFTTERREYTKNKIMVDGNTACG
                     LGAIYGGATVAAWYPITPSTSVVEAFEKYAKKYRVDPETGKNKYAIVQAEDELAALGM
                     VIGATWNGARAFTATSGPGVSLMNEFLGLAYFAEIPAVLIDVQRTGPSTGMPTRTQQS
                     DILISAYASHGDTKHVLLFPSTPKECFELTAAAFDLSEQLQTPIILLTDLDLGMNDHV
                     SDPFEWDDDRKYDRGKVYHAEDLEKIEKFGRYLDIDGDGIPYRTYPGAHPKKGAFFTR
                     GTSHNEYAGYTEDGADYTRNVERLLVKFETAKKLVPKPDFYQKSNKSDEAMLFFGTSM
                     YPAEEALDVLKENGIELDAVRIKAFPFNDDIVEFINAHEKVYVIEQNRDAQLKTLLVT
                     EHGFDPAKLVSILNYDGMPMTAEQISKAILEKSKSTIAKTVNN"
     gene            complement(11645..13435)
                     /locus_tag="PLE23_01475"
     CDS             complement(11645..13435)
                     /locus_tag="PLE23_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX81964.1"
                     /translation="MKPTDIKNPEYFHKVVDCQYACPSHTPVPQYIRLIAAERYSEAY
                     MVNWESNVFPGVLGRTCDRPCEPACRRGRVEEKPVAICRLKRVAADNKDEVKQYMPAG
                     PFAPNGKKIALIGGGPASLTVARDLSPLGYEIHLFDEHKKGGGMMRSQIPSFRLPEEV
                     LDEEVGYILDMGVHTHFNKYVSSLKEIIEDGYNAIFVGTGAPRGRDLASLPGRQEGKS
                     NIHIGIEWLASVAFEHIHKIGKKVIVLGGGNTAMDCCRTARRMGGEEVKVIVRSPFTE
                     MKASPWEIEDAIHEDIPILENHVPKEFVIENGKLKGMLFDKVHAVYDEDGKRSLVPTA
                     EPPVFVEADDVLVAIGQENSFPWIERDLGLEFDKWNMPVVDKTTHQSTVSHIFFGGDA
                     AFGPKNIITSVAQGHAAAVSIDLFCSGEDVHVRPSPVTNLVSQKMGIHEWSYDNYISN
                     DFRFVVPQADKSITLKNLKKEVELGFDVESAFKEAQRCLNCDVQTVFDFKRCIECDAC
                     MDICPTSCITFTTNGDESDLRTRLKAPSNNINQDLYVSEALPTTRVMVKDEDMCLHCG
                     LCAERCPTAAWDMQKFSYITSKPGYSWQAV"
     gene            complement(13694..14419)
                     /locus_tag="PLE23_01480"
     CDS             complement(13694..14419)
                     /locus_tag="PLE23_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMX81965.1"
                     /translation="MSRINVIIVDDEPLALDILETFVKKVPEFELIGRCNNAAEAISM
                     IDANKVDVMFLDIQMPHMTGIELMRSLDDPPLVVFTTAYPNYAVEGFELNALDYLLKP
                     ISLERFMKAVHKIKDHHSIQKENDNELNEDKDFFFVKADKKLMKINYNDITYVEGLKD
                     YVILWLGDTRIITLQTMKSLEIKLDSKNFRRIHRSFIVNLNKIKALNGNMVEVEYKAL
                     LRQLPIGKNYKDDLLEIIGESRL"
     gene            complement(14416..15462)
                     /locus_tag="PLE23_01485"
     CDS             complement(14416..15462)
                     /locus_tag="PLE23_01485"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMX81966.1"
                     /translation="MLETIISFLTRRKIGQHLLFWLFSYVFLCFMEYLTTRGTFHSIL
                     LKQLINIAFYAIMVYGNIYYLIPNFFREKRYQSYFLILVLYVLIITPIFSFFLYIAAF
                     DDPFQKAELLKLKNYYYFFNLFIIGASTVFVIIKDWILQKREKRELENRNMQTELNFL
                     KSQINPHFLFNTLNSLYALTLKKDDKAPDVVVKLSEMMRYMLYECNEKYVPLEKELNY
                     LHNYIDLEQLRQPDRVQIKMTVKGDPSDLEIAPLILISFLENAFKHGVSRSIGKAHVN
                     VCIAIKDKNLNLEIENSKPKQKTDQLKEPGGIGLQNTKKRLDLIYGGRHVLIIEENSE
                     IFTVDLSINLTQDS"
     gene            15615..16208
                     /gene="folE"
                     /locus_tag="PLE23_01490"
     CDS             15615..16208
                     /gene="folE"
                     /locus_tag="PLE23_01490"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HMX81967.1"
                     /translation="MSLESETPMNSEVLQMKDKFRDIIGLLGDDVNREGLLKTPERVA
                     KAMKFLTSGYEQDPSEILRSALFHEDYNEMVIVKDIELYSMCEHHMLPFFGKAHVAYI
                     PNGKIVGLSKIPRVVDVFSRRLQVQERLTHDILNCIDNVLKPHGAAIVIEAHHMCMMM
                     RGVQKQNSVTTTSAFTGEFKRNETRNEFLKLIVAKLD"
     gene            16250..17125
                     /gene="fabD"
                     /locus_tag="PLE23_01495"
     CDS             16250..17125
                     /gene="fabD"
                     /locus_tag="PLE23_01495"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HMX81968.1"
                     /translation="MRAFVFPGQASQFVGMGKDMYTNNESARILFDQADEILGYQISK
                     VMFEGTEEELKETRITQPSVFLHSVIKALINPDVVPDMVAGHSLGEFSALVVNGCLDF
                     ASGLLLVKERAEAMQQACELEQSTMAAILGLEDNKVEEICNSINHTVIAANYNCPGQL
                     VISGSIQGVEEAMKACMEAGAKRAIKLAVGGAFHSPFMQPAQDRLKAAIEQTQFSEPF
                     CPVYQNVNATPSTDPDVIKKNILIQLTAPVLWTQIVRNMITDGCDHFTEVGGSGSVLR
                     GMIRQIDRNILSDAL"
     gene            17134..17691
                     /locus_tag="PLE23_01500"
     CDS             17134..17691
                     /locus_tag="PLE23_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMX81969.1"
                     /translation="MEHQKISQGSLLLAEPFMLDVYFRRAAILICDKHEEGTLGFILN
                     KPLDVKVDSIINDFPEFDALAYYGGPVQTDSVHFLHTAGDIIDDSQFVCDGIYWGGNF
                     EKVKVLITQGLLHPGNIRFYVGYTGWSKYQLEAEIEDKTWIIDEMDPNYLFVFKPDKL
                     WQVILENKGNTHAILGQIDDFLNLN"
     gene            complement(17695..19032)
                     /locus_tag="PLE23_01505"
     CDS             complement(17695..19032)
                     /locus_tag="PLE23_01505"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMX81970.1"
                     /translation="MKLTNIVSQTLNKVRLIPAPISITLVSGLLMYLVNYAVKDSSTD
                     FSLKASLSELSAFPLEMPNVKYELLLDTMEVRTTLIKPNQFLVDILKNYELSDNEINN
                     MVAKAAKVFDLTKLRAGKEYSVMCKKMTEKPYYLIYEPDAYGYVKLDFRDTMQASYIQ
                     KNIQTNIKIAKGIVNSTLSESMAEGGLSYDLIDKMEDALAYTVDFYHVQKDDKFKVLY
                     EDHSIDGKSVGVGKMYGAYFEQNGKDYYAIYYKKGDREGYYDLEGRPMKRYFLKAPVK
                     FSRISSGFSLRRFHPVLKYNKPHFGTDYAAPAGTPIMSVGNGVVIEASRNGYNGNYVK
                     IKHDATYTTQYLHMSRIGKGIRKGVRVSQGQTIGYVGSTGLATGPHVCFRFWKNGVQC
                     NHLKEKVSSNPVMNKADLPEFFKVRDEVVRKLNIVKHKPVTESIQSETKDDNP"
     gene            19290..20168
                     /gene="folP"
                     /locus_tag="PLE23_01510"
     CDS             19290..20168
                     /gene="folP"
                     /locus_tag="PLE23_01510"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMX81971.1"
                     /translation="MISPVTSFNCRGRLLVFEDPIIMGILNITPDSFYAASRVTGTDE
                     IIDKAGQMIEDGATILDLGAMSSRPGAVNIDTDAEISRLMPFLSAVRDAFPQVFISVD
                     TFRAEVAKQACSAGADIINDISGGRFDENLFRVIADANVPYVLMHNGATFESMHSKKG
                     LGDPVATVFDFFTEKIRQLNEYGIYDIFLDPGFGFGKLMQENFSLLKSIGYFNILHLP
                     VLAGVSRKKMVYTSLNISSDDALNGTTALNMYALAQGSKVLRVHDVKEASQSIELYKL
                     IRDSDKEICAESTLYE"
     gene            20504..>23619
                     /locus_tag="PLE23_01515"
     CDS             20504..>23619
                     /locus_tag="PLE23_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81972.1"
                     /translation="MELASVRFNFFNNFELRGFSIDDLHANPVIYAQQLQINFSVNPV
                     NLYRQRIVLDEVVLKNAGVNLRKYPGETEQNIDLLIRKMFPPKEDKKKSDTKLKLIIK
                     TIQLAVVEFTDYNEHNGNYLSISTQRGEAKVGKMDFVNKEYIFSSVIFDMPVFRLQYR
                     KLSMEAIEASGKVIKETDNPNGKVLKLVFQDFELNDGSLFVDNYQRPPDPNIKISVDD
                     IDWSHFAFNDVHAIGKNLIIHDGSYSASPLKISLKESKGFEIKELTAGFAGINNEQIR
                     LAELNLQTKASVIRDSVILNYESLSSLSDDFANNVGIKIGLNKSMISVREIAHFAPSL
                     KYDKFFVNNFNEVLTVSGEIYGRINSLKAKNLIVEIPSKLYLHTEFKSNDISQTDNAI
                     INLDIKQLNSNVQFLEKAIPNLVLPPNFSKLGELSFKGNFDGFFTDFVTYGTLKTNLG
                     DIGLDLRMNLQNGKDNSIYSGGMKLKNFNLGSWIDQNKFGNLSADFNVRNGHGFTADK
                     AEADLDGKVHSIEYNSYTYHDIVLNGIFKKNTIDGSITGSDPNADFTFTGKVDLGADI
                     PEYDFLSEIKNLDLYKLNLSSKPLAVSGNLDINLLTRNFKTLDGTSKINNLRFTNGQK
                     TLFELDEILLNASQTGNVKTIDVKSDVADVYISGEFEIDNLHQIFLQNLKKLYPRYYD
                     DIKLPKVNLPFTRTQCSYKFDIKKTEPLLALIDNPVSFDGPLLVSGKFNNLTNQWNTL
                     ATTEGIKVGDKVFKGITINYIQYDEEANFSGILDKTVVMDKVLDNTRFTFVINPENIK
                     FDFNGIKLYSSDLSVNGTLKPTDDGYNLHFNNNSINLLGENWEVNPDNLLIINKQTII
                     PESFVLTNENRQIILNQTINDGLEFAIIGYDVSKLNEIIKYDKLKLSGNLQIYARVNE
                     VYKLRNFGLRVYSDSLNINKDHWGKLLVNASAIDFKELLNVEYQLEHDTIAMSGRGFL
                     NISDRENIRLKLDNRFNYLPLRTLEYFIGNGINNTSGSLKGNFTVEGPLHNMGLSGKA
                     DIAKAGFH"
BASE COUNT         7252 a         4504 c         4603 g         7260 t
ORIGIN      
        1 tcattgctag caatgtgact tctgccccaa gctcggtcat agcttttgct aaatgcgtaa
       61 tagctattga ttcaccatct tttaatggaa atgggaattt tttacaaatc tgaagaactc
      121 gcatacacaa attttttttc cagtgtcaaa caatacaaaa tcgaaacaaa agtcactaaa
      181 aatattgtgt agagtttcgc gatagcgttg atttaagtga ttgaattaat tcctgaaagc
      241 aaatttaatg aaaactaaaa tattttgcat gttttatcct ttattttttc aattatttta
      301 agattttaat aaaaaagact attcttgcat tgaaaatagt agcggatgtt tctcttggca
      361 gaaaatataa agaaatcgta cggaacagtt aatgtgctga aaggtattga tgtgaacatt
      421 gaaaaagctg aattcgtaac aatcatcggc aaatcgggtg ccggcaaaag taccttgctg
      481 catatattgg gtacgctgga taaagctgat gaaggtaagc taattatatt cggtgaagat
      541 gtaacaaggt attcttcaga caaactggct gattttcgca atcgccacat tggctttgta
      601 tttcagttcc atcatttgct acctgaattc actgcttatg aaaatgtcat tattccggct
      661 atgatagccc gaagacgtga gacagattac agaataaaag ctgagcaatt acttgattat
      721 ctcggactta cagatagaat gggacataaa ccaggggagc tgtcaggtgg tgaacaacaa
      781 cgggtcgcta tagcgcgtgc gcttataaac gatccttcta taataatggc tgatgaacct
      841 accggcaatc tcgatagtac aacctcggcc gaaatccatg aactgatagc tcgtttgcgc
      901 aaagagcttg gcgttacttt tatcatcgtc acacataata ttgagctgac cgaactcagt
      961 gaccgaagcc ttaaaatgtc tgacggactc attatcaaaa cgtagaatct atgataacat
     1021 attcctgcaa aattgcttgt gcccccattc gcctcgaaca aagcgacaaa tctgagatga
     1081 tttcccagtt actatttggc gaaactttta aaatcatcaa aacaacaaag tcttggaaaa
     1141 gaataaaaac gcacgacggt tatgaagggt ggatagaccc gcgtgtcatt gccaatacca
     1201 aggcagtaat taaagaaaaa tgttatgtaa acaaactttg ggcacatgtc atttctagtg
     1261 gaatggagat gtttgtgcct tatggttctg aacttaatcc tgaaaaggat gagttcagta
     1321 tccatgattt tacattcgtt accaagtctg gcccggagaa gatcatacaa gatgctttgc
     1381 tgtttttgaa tacgccatat ttgtggggtg gccgctcgtc ctttggggtt gactgtagcg
     1441 gactcatgca agtgttggca aaagtaaatg gttatacact ggctcgtgat gctagccagc
     1501 aagttaattc gggaaaagct gtagaatatg gcaaacacaa catcggagac ctggcttttt
     1561 ttcaagacga atccggcaaa attgtacatg ttggtatcct gtcagccaaa gatgaaatca
     1621 ttcatgcttc cggatgggta agatccgatt tatttgatga aaaaggcatt ctggtcgaat
     1681 caagcagttt gtactcacat catttggcct gcattcgtag aattttctga aattaaatat
     1741 ccggagctac cgaagattga tatcatcgtg atcaatcaag taattcagga aaatcaaggt
     1801 gaaactgtac aagatcatca atgggtgaac ggatgatttt tccggtgctc ataccatatt
     1861 tttcagctac agaacttaga atatcggcaa aattgaagcc aactgagcct acacaattaa
     1921 aactgtaaga ccgatattcg ggatatttac ttacaagatt ttcgaagaat gaggtaaacc
     1981 cttcatgaac aatattcgaa cagtaatcca aatggcgatt atcgttgaca aattttgcga
     2041 agcttgcaag gaagcggttt ggcaatggtt tattgtacaa agcatcaagg atgtcttcgt
     2101 tactaagtcc gaatgtgtct aaaaaagtct tatgaagttc tgccggcaag aatcgacgca
     2161 tataatcacg aagcagcttt ctgccaatgt acgaaccact tccttcatca ccaaggaaat
     2221 acccaagtga gtcaatattg agggtgatat tttttccatt gtaaagacaa gtattggtgc
     2281 ctgtgcctag gatggctgca aatccggtag tgttgccaag tagtgccctg gctgatgcca
     2341 gcaaatcgtg tccgacaaaa acttttgcgt ttttgaacac attttgcatg gctatttcca
     2401 ctattctatt tttttcgggt gtagaacaac cagctccgta gtaatatatg tgcttgattt
     2461 ggtacttgtt cagctctcca ggcaaactcg attcgagcga tttgataata ctttgtgtat
     2521 cagcaaaata gggattataa ccttcagtat taaaataatc attcggcaaa ccgtcacctt
     2581 ttacaagaca ccagttgctc ttagtactac caccgtctgc tatcagcatc ataattttcc
     2641 gttgttttga ttgttgtata gattaaacct gagcatcgta gtcaattccc tggtttttta
     2701 gaattcgttt aacaataaat gcaaaaaatg caagatagcc aaagcaaaca actgtcaccc
     2761 aataagactg gtgtattcca atagaggaaa tatcggcaag cttgccctga ataggcggga
     2821 tgattcctcc accgagtatc atcattatca gaaaagccga accttgggta gtatatttgt
     2881 tgagacctgc aagtgaaagg gagaaaatat ttggccacat aatagaacaa aacaagcctc
     2941 cactcaagaa agcataaata gcaaatgtgc ctgtactaaa aatgcctacc agcatagcca
     3001 ccacacccaa aacagaaaaa agtaacaaca tcagagctgg tttgtcttgt gaaatgtaaa
     3061 atgccaccat ctgaataaag acacaaatta tgtaccaata taaggcagac atatcttttt
     3121 gagcaataga atttacaaga atgatgagac cgaaagctac aataggaact actaccagaa
     3181 gaagattttt caaacttttg ccaggactga acaccgggat ggcacccacc cacctgccta
     3241 tcatcaggct gccccagtac atagagatgt agggcgatgt ttcggatgcc tgtttgcctc
     3301 caaactcagg gagttttagc aattcaccta agttgctttg aatagaaact tctacaccaa
     3361 cgtataaaaa tatggccagc ataccaagta cgagttgcgg gtatttcatt gcaccccaac
     3421 cggcctgatt tttcttagca gactgccatg aaccaagcaa accaaacact acaacagcaa
     3481 gtgcgagaag caaccatttc aatctacctg tttctagaag gtgcctggca tcttctgaaa
     3541 tgtctgtatt gacaccaaca taacttttaa agacaggaat aaaacagaga atgagcaatg
     3601 cagtaataat caataaggta cgtgttgctt tatcagcttt ctcaagtttt tcatcaataa
     3661 tgcctgacgg tactttttta gaaaaaatga agagcaaggc acaaagcaaa aataaaccac
     3721 ctacaaaagc atataatatt gtaactgtac ccaatccaag tgactgaatc tcactgtcag
     3781 aataaacagc tgcgccgaat aaggcatacg atacaattat tggaccgatc atagtgccaa
     3841 atgagttaat accacctcca aggtttattc tttgagagcc ggtagccggg tcgcctaatt
     3901 cgatcgcaac tggttgggct gctgtctgtt gtaacgaaaa tccgagagct acgataaaaa
     3961 gtgctaccaa aataccggta aagcctccaa aattgattgc cagaatcatt gcaatggcac
     4021 ccaataacga gaacagcaaa ccgtaaataa tacttttctt atagccccat ttaccaacca
     4081 ggtcttttcc acccatggtg ccaaaaaaga ataaacctaa cgctcctaga taataggcac
     4141 cataaaacgc aaagtctatt agttggcttt gaaactggtc gagatgaaaa taatgcttac
     4201 aaaatggaat aaatacaccg ttggaagcgg caatgaatcc ccaaaagaag aatacggtaa
     4261 tcagtgttgt gagagcaccc gattgggttt taacatgttt gcctgtttcc attgagtatg
     4321 ttttttggtt ttgactggtt taatttgttt agttgttcat cacgaagccc aaatataaaa
     4381 gcaagtgatg ataaaaacaa agtgtgttac catcttttgt attaatattc tgattttgcc
     4441 caaatagtcg tttggcgttg aggtataata tgactaggat tagcatatat ataatacaaa
     4501 taaaaaccat tacgtattac ggaaccagta attgtcagag ccaagaagcg aatatattgc
     4561 agatgtggtt tggataataa ttgtgtaaaa taaaaaaagc ccgttataag aacgagcttt
     4621 aaaaggaagc ggagagacag ggattcgaac cccgggacct gttacagtca acggttttca
     4681 agaccgccgc attcgaccac tctgccatct ctccaaagat atttttacaa tcagataacg
     4741 ttctatcgtt tgtgcgtgca aatatataac cattttgtaa aaaaagaaat catagataaa
     4801 aaaagttaaa aaaatttatt tcaaaagaga acaatcacat gaccaattag tgtgaagact
     4861 cgaggcacat acatcaatca tatttgaacg aagcatctgt aaagtgaata aacaatattg
     4921 ttgttcaatc tagttattga agcttaattt tgatgaaata attgctgaac tgtaagtgca
     4981 caaaaaatga aagagcaggt tttataaatt tggttaaaat agaactcaga attttagtaa
     5041 aaaatatttg aaaaaaaaat ttttaatttt gttttattta aatattggaa ataaaatatt
     5101 gattttgaaa ttttctaaat aaaatttgct aaaattatac agaaaatcta aatttgcaat
     5161 tgtactaacg aactatttta aatcatttta agcaaaacca aatttatatg aaacgaaatc
     5221 tacttgcgtt tttcatgttc gttttggcat taggtttttc taaccgaaca atggctgtat
     5281 tgcctgatgg ttcggatgcc aaagactgga cattgacaga cattaatggt cagacctatc
     5341 atttgtttga cttattgaat caaggcaaaa ctgttttcat tgatttttca gctacctggt
     5401 gcgggccatg ctggaattat cacaattcag gagctcttga aaatctctat gaaagttatg
     5461 gccctccggg cagcaacgaa gttatggtat ttttcattga agctgatttg aatactaacc
     5521 tcgcatgttt gtacggacta acaggttgtg ttggtggcac acagggcaac tgggtggctg
     5581 gtacacctta tccaattatt gatttggtac catctacagg ttatgtagcc agtgattaca
     5641 gcatttctta ttatccgact atttatgcaa tttgtccaca gacaaagaaa gtgtacgaat
     5701 ccggtcaggt acctgcttct gtacattatt cttatgtttc ttcttgtgca ctagattttg
     5761 tagtagataa tgtagttgac gccaagtgta atggcagtaa aaatggtagc atcagtatct
     5821 ctacccagca gggtagtgcg cctttttctt ataaatggag taatggtgcc aagacacaag
     5881 acatcgttaa ccttgctgca ggttcatatt atgtaacgat tactgacaaa aacggtatat
     5941 tcgtagaatc tcaggaaatc atcgtcggtg aacctgatgc tgttgaagtg caggctacag
     6001 tagtagatga gggctgtgaa ggaacagcaa ctggagaaat aacagcagat ggctcgggcg
     6061 gtaaccccgg atatacatat tcgtggagta atggaaaaaa tacagctact atcaccggac
     6121 tgacacaggg tgattatact gtcatagtta ccgatgccaa tggatgtacc gcgacgaata
     6181 cttatagtgt gtctgctcag ccattgccgg aggcctatgc cggaccggat gacttgctca
     6241 actgtgtaca caatacactg atacttgaag gtgaagctga ttttggttca gaatttactt
     6301 acgaatggtc aacctccaat ggacatatag taagcggtgg aaatacgctg aacccagaaa
     6361 tagataaacc cggaacttac aaactcctag taacaaatac ttcgacaggt tgcgcaagag
     6421 aagaccaggc tgtgataaca agtgattata ccactaccca aacagatgcc ggagacgatg
     6481 gactagtcaa ttgtacttcg ccatctattg ttctggacgg gagtaaatca tcaaaaggtg
     6541 ctaatatcca gtatctttgg actacgggca atggtcagat tgtaagtggg ggtaatacta
     6601 acaaagctac tgtcaataaa ggtggaactt acaaacttac taccacacat gcagtgagtg
     6661 gttgtacttc tgaagatgaa gtcgttgtta ccgagaatct aacaggtaca acagctgtac
     6721 ttgcaccagc aggagaaatt aactgtgaaa accctgaaat cacattggac ggaacagctt
     6781 caagccaagg tgcggatgta gaatattcat ggacaacgaa tggtggaaac ttcactagca
     6841 gcacagatac taattttgta aaagtaaatg ctgcaggtga gtatttcctc attgtaacca
     6901 ataagaacag tctttgtaaa gatactgcaa atgttgttgt tgtatctgac ggtgaagtgc
     6961 cagtggcaaa tgcaggatct ggctctgagt tgaattgcaa cgttactact atccagcttg
     7021 atggtacaaa atcaagccag ggtgcggatt ttgatgttaa ttggagcaca caggatggca
     7081 acattctttc tggagcagat acctattcac ctacggtgaa tgcgcctggt acatacgtta
     7141 ttgcaattac aaatactgtt acaaactgta cttcttattc atctgtagtt ataacagaag
     7201 atgaccttac caaggtacct gacgctatat ataactatac ggccaatttg ttaaatctta
     7261 acttccagga tgcttcaacg ggagtccctt caacatacgc ttgggatttc ggtgacggtc
     7321 agacttcaac agaacaaaat cctactcata cttatttact cgagggcact tatacaatat
     7381 gccttacagt aaccaatgcc tgcggtacag accagagttg tcagaccatc actgtatcaa
     7441 gcagcttaaa tctgcctacc atcagtgacc tgaaaattac tgacgttact tgtaaaggtg
     7501 gtagcaacgg atgtattgac ataacagtaa ttaacggtat tccgccttat acttttgctt
     7561 ggagtaacgg tcttaccacc gaagatcctt gtaatattcc tgcaggatct tatgctgtta
     7621 ctattactga tacagacggc aaccaaactg tttcggcatc catcagtatc aacgaactgt
     7681 attatatcaa tgttaataat acgtttgtta cagaacctga ttgtaatgat aacaatggct
     7741 cgatagctct tgatatcaac agtaatggag gtcaattgac ttacgcttgg tcacatgatt
     7801 ctactttaaa caatgcggtg gctgaaaatc tacctgaagg caactattct gtaatcatta
     7861 ctgatgccaa tggttgtaca agtcaacaat cggtagtcgt tactgataat ggtcagcatt
     7921 tagatggtag tgcttcatct cttctttgct ggggtgatca gaacggtcag atacaactga
     7981 ctgtaagtgg aggaacccag ccatacactt tccagtggaa taatggttct acaactgaag
     8041 atcttgccaa cttgtcaagc ggcacttata cttgtaatgt aaccgactca ttgggttgtg
     8101 ttaaaactat tcagcttgtg gtgtcatctc cagatcagat tactgctgat atggcaactg
     8161 taaaaccaca aaaaggtaaa gataacggtt cgattacggc tacggttgcc ggtggtacag
     8221 caccattcca gtatttatgg aacaacggct ctaccgatcc tgttatcact aatcttgcac
     8281 ctggcactta cacacttaaa gtaattgatg caaatggatg tatcagagac tttgatgtag
     8341 ttttggaaga ggcagtaggt actttatcac ttgatattct cgagcaagtg aatgtttacc
     8401 ctaatccaag taacggcaac ttctggatcg aagctacttt tagcaaaaat acagttgttg
     8461 acttaacgat tcttgatctg ctcggtaggg aagtggtgaa ccaacaattt gaaggaaatg
     8521 aattcaaatc gcagattgat atgcacaact tcaacagtgg aacctacttc attagaatct
     8581 ctgctgataa cggaatgcac gtagaaaaga tgagtattgt caaataacat tttcactaag
     8641 ctacggctaa tattagaaaa gtccctctat tcatttagag ggactttttt attttaagaa
     8701 tatttgctga tgtgttttat tcgtaagtgc tttactcaga atattagtac aagcaacaga
     8761 ttaaaaaatc atctgaaatc atcatttact tctgcaagtt ttgctgaccc cggcgacaat
     8821 tcattagctg aaaattgatg taatggtttt tcgttggttg tgtttaacat ggtattaagg
     8881 tctgtaggat tttgctcaaa ataaatcaat ccggtaagaa tttcctgact gttacgagct
     8941 ttttgtaaag cagcaaatga tgaatcacgt tcgagaggat tccagtctgt gtcgagttta
     9001 ttaaattgaa ggtatgagcc gtcgtgcatt ttgatattcg tctgtgtacc ttccttatag
     9061 tcagctacta tttcattatg tgtagggacg aaatctattg tacctgtggc atctatgtgt
     9121 tgacgtacat aatcgtaagc ctttgtagaa ccgacattat tattgaacgt aacacatgga
     9181 gaaataacat taataaatgc aaagcctttg taattcatgg ctgcttcaat aagtggtatc
     9241 agttgggttt tgtcaccaga gaaactttgt gcaacgaacc ctgcgccgag ttcaagtgcc
     9301 agacctgcaa aatctattgg ttgaaagata tttacagagc cagctttact ttttgagcca
     9361 acatcagccg tagctgaatc ctgccctttg gtgagtccgt agcaaccatt attcattaca
     9421 atatatacca tattaatatt gcgtctgaca gcatgtacaa actgccccat gccaatagaa
     9481 gctgaatcgc catcaccgga aacgccaaaa tacattagat cctgattaac aagactggct
     9541 cctgtggcca ccgaaggcat acgtccatgt acagaattga acccgtgcga attgccaaga
     9601 aaatacgttg gagtctttga agaacaacct atacctgaaa gttttgctac cttgtgtggt
     9661 gcgatattct gcctgaaaca agcttcaaca atagcagcac ttatagaatc atgcccacaa
     9721 ccggcacata atgttgacaa tccaccttca taatccttga aggtatatcc cagtgaattt
     9781 tttggcaaat ccgggtgttt gaaagttggc cttaggtacg tcatgatata ttaattattg
     9841 actgttttag caattgttga tttagatttt tcgagaatgg cttttgatat ctgttcggct
     9901 gtcattggca taccgtcata attcaagata gaaacaagct tggcgggatc aaatccatgt
     9961 tctgtaacca gaagtgtttt tagctgtgca tcccgatttt gttcgataac atacaccttt
    10021 tcgtgtgcat taataaactc tacgatatca tcattgaacg gaaatgcttt tattctaaca
    10081 gcatctaatt cgataccatt ttctttcagt acatcaagtg cttcttctgc cgggtacatt
    10141 gaggtaccaa agaatagcat tgcttcatcc gatttattgg atttttgata aaaatcaggt
    10201 tttggtacaa gctttttggc tgtttcgaat tttacaagaa gtcgttctac attccttgta
    10261 tagtctgcac catcttctgt gtagccggca tattcattat gcgacgttcc tcttgtgaag
    10321 aatgcgcctt tctttgggtg agcaccagga taagtacgat acggaatacc atcaccgtcg
    10381 atatcaagat atctaccgaa tttttcaatt ttttccagat cttcagcatg atataccttt
    10441 cctctgtcgt attttctgtc atcatcccat tcgaatggat cactcacatg atcattcatg
    10501 ccaaggtcta ggtcagtcag caatataatc ggagtctgga gctgttcgga aagatcaaaa
    10561 gcagcagcag tcaattcaaa acattcctta ggcgtactcg ggaagagtag tacgtgcttg
    10621 gtatcaccat gggaagcata ggcagaaata aggatgtctg attgttgtgt tctggtaggc
    10681 atacctgtgg aaggacctgt gcgttgaaca tcaatcagta cagccggtat ctcggcaaaa
    10741 taggccaggc caagaaattc gttcatcagt gaaacgccag gaccagaagt agcagtgaaa
    10801 gcccttgccc cgttccaggt agcaccgatg accataccca aggcagcaag ttcgtcttct
    10861 gcctgcacga tggcatattt gtttttgcca gtttcgggat caactctgta ttttttagca
    10921 tatttttcaa atgcctccac cacagaggta gaaggtgtaa tcggatacca tgctgctaca
    10981 gtggctcctc cgtatattgc acccaagcca caggcagtat taccatctac cattatctta
    11041 ttctttgtat attctcttct ttcagttgta aatgggaagg tatgattaaa attttccttt
    11101 atataattta tgccgagctg caatgcctga atattcggag ctattaattt ctcctttcct
    11161 ttaaattgat ctgcaaaaag gtcttcatat acattgaatt caatatgcaa cagttcggta
    11221 agtgcgccta catatattat atttttaaat aattgtcttt gtcgagggtc agcgtattcg
    11281 cggttacaca tttccattag cggaatcccg atgtagttga tatcttctct tacaaatgaa
    11341 ggatgtagtt tttttgtatt atcgtagata aaatatccac cggtctttac actttttaca
    11401 tcttgcatca tactttgtgg gttgacacaa accatcacgt ctattccttc tcgtctgcca
    11461 taataggctt tatcactcac tctcacttca taccatgtag gtaatccctg aatgttagaa
    11521 ggaaaaatat tttttgccgt taccggaata cccatacgaa atattgcctt tgcaaacaga
    11581 tagttagcac tggcagagcc tgtaccgtta acattggcga attttacaac aaaatcattt
    11641 acaattacac tgcttgccat gaatatccgg gttttgatgt tatgtaagaa aatttttgca
    11701 tatcccaagc tgcggtaggg cacctttcgg cacacaatcc acaatgcagg cacatatctt
    11761 catcttttac cataacccgt gtggttggta atgcttcaga tacgtataag tcctggttaa
    11821 tattattgga cggagctttg agtctggttc gcagatcgct ttcatcacca ttggtggtaa
    11881 atgtgataca tgaagtaggg cagatatcca tacaagcatc acattctata caacgcttga
    11941 agtcaaatac tgtttgtaca tcgcaattaa gacatctttg cgcttctttg aatgccgatt
    12001 caacatcaaa accaagttcg acctcttttt taagattttt cagagtgatt gatttgtcgg
    12061 cttgtggcac cacaaatctg aaatcatttg atatataatt atcatagctc cattcgtgaa
    12121 tacccatttt ttggcttacc agattggtca ccggtgaagg ccttacatgc acatcctcgc
    12181 ccgaacaaaa taggtcaata gaaactgctg cagcatgacc ctgagctacg gaggtaatga
    12241 tgtttttcgg tccgaaggcg gcatcaccac caaagaatat atgcgataca gttgactggt
    12301 gtgttgtttt gtccactacg ggcatattcc acttatcgaa ttctagacca aggtcgcgct
    12361 ctatccaagg aaatgagttt tcctgtccaa tagctaccaa cacatcatct gcttctacaa
    12421 atacgggagg ctcagcagtg ggtacaagtg atcgtttgcc atcttcatcg tacacggcat
    12481 gcaccttgtc gaacaacata cctttcagtt tgccattttc aatcacaaat tccttaggta
    12541 catggttttc aagtatcgga atatcttcat ggatggcatc ttcaatttcc cacggtgagg
    12601 ccttcatttc agtgaacggg cttcgtacta ttacttttac ttcttcacct cccattctcc
    12661 ttgctgttct gcaacagtcc attgcagtgt ttccaccacc cagtacgatg acttttttac
    12721 ctattttgtg aatgtgttca aatgccacgc tggctagcca ttcaatacct atgtgaatat
    12781 tggattttcc ttcctggcga ccgggcagtg aggcaagatc tcgtccacga ggagcacccg
    12841 tacctacaaa aatagcattg tacccatctt caattatttc tttcaggctt gacacatatt
    12901 tgttaaagtg tgtatgaaca cccatgtcaa gtatatatcc tacttcttcg tcgagtactt
    12961 cttcgggtag tctaaatgaa ggaatttgcg atcgcatcat tccgccacct ttcttgtgtt
    13021 cgtcaaataa atggatttca tagccaagtg gagagaggtc gcgagctacg gtaagcgagg
    13081 ccgggcctcc tcctatgagg gcaatctttt ttccgtttgg agcaaaaggt ccagcaggca
    13141 tgtattgttt tacttcatct ttgttatcag cagctacacg tttcagacga cagatggcta
    13201 ctggtttttc ttcaacccga ccacgtcggc aggcaggctc acagggccgg tcgcatgtac
    13261 gcccaagcac accagggaac acatttgact cccaattgac catatatgct tctgagtatc
    13321 gttcagctgc aataagtctg atatactgag gcactggtgt atgtgaagga cacgcatact
    13381 gacaatccac tactttgtgg aaatattcag gatttttgat atcggttggt ttcacaattt
    13441 tttctgtttt aataagtgtt aattcttaat ggctcaaaag tataaaatat atccaacaaa
    13501 gccaatctga taagcctcga aaatgcagtt atgttaaact ttattttatc ggcatgtttt
    13561 tttgttagga tttagttaaa gttcttgatt ttgaaatgta aaggtggtta acttcgttga
    13621 tatttttgat aatggttttc agttggtttg tttgttagtt tccagggcat catttcttga
    13681 ccccggattt tttttagagt ctgctttcac ctattatttc aagcaaatca tccttataat
    13741 ttttacctat tggtaattga cgcaataggg ccttgtattc cacttctacc atattgccgt
    13801 tcaatgcctt aattttatta agattgacga tgaatgaacg gtgtattctc ctgaaattct
    13861 ttgaatcaag ttttatttcc agactcttca tggtttgcag agtaatgatt cgggtatcgc
    13921 ctaaccatag aatgacataa tcttttaggc cctcaacata ggtaatatca ttgtaattaa
    13981 tcttcatcaa ttttttatct gcttttacaa agaagaagtc tttatcctca ttgagttcgt
    14041 tgtcgttttc tttttgaata gaatgatgat ctttaatttt atgtactgcc ttcataaatc
    14101 gctcaagaga tattggcttg agtaaatagt cgagtgcatt tagttcgaat ccttcaacag
    14161 cataattagg gtaggctgta gtaaatacta cgagaggagg atcgtcaaga cttcgcatca
    14221 attcaatacc cgtcatgtgt ggcatttgaa tatcaagaaa catgacatct accttgttgg
    14281 cgtcaatcat gctaattgct tctgcagcat tgttgcaccg gccgataagt tcaaactccg
    14341 gcactttttt tacaaatgtc tcgagaatat cgagtgcaag tggttcgtcg tccactatga
    14401 ttacgttaat tctgctcatg aatcctgtgt taagtttatt gataaatcta ctgtaaatat
    14461 ttctgaattt tcttctatga tgagcacatg tcttccacca tagataaggt caaggcgttt
    14521 cttagtgttc tgcaatccga tacctccggg ttcttttagc tggtctgtct tttgctttgg
    14581 ttttgaattt tcgatttcca aattgagatt tttgtctttg attgcaatgc agacattgac
    14641 atgagcctta ccaatactcc ttgacacacc gtgtttgaaa gcattttcta ggaatgaaat
    14701 aagtataagc ggtgcaatct ctaggtcgga agggtctcct tttacggtca ttttaatctg
    14761 tactctgtca ggctggcgaa gttgttcaag gtctatataa ttatgaagat aatttagttc
    14821 cttttcgagt ggaacatatt tttcattaca ttcataaagc atgtaccgca tcatttccga
    14881 caattttacc accacatcag gcgctttatc gtcctttttg agtgtcaagg catacagact
    14941 attgagcgta ttgaacagaa aatgcggatt aatctgcgat tttagaaaat taagctccgt
    15001 ctgcatattt cggttttcga gttccctttt ttctcgcttt tgcaatatcc agtcttttat
    15061 gattacaaaa actgtacttg caccaattat aaacagattg aaaaagtaat agtagttctt
    15121 tagtttgagg agttctgctt tttgaaatgg atcatcaaag gcagctatgt atagaaaaaa
    15181 tgaaaaaatg ggcgtaataa tcaatacata caacacaaga atgaggaaat atgactggta
    15241 gcgtttttcg cggaaaaaat taggaatcaa ataatagata ttgccataga ccataatggc
    15301 ataaaaggct atatttatta actgcttcag caatattgaa tgaaatgttc ctcttgtagt
    15361 aaggtattcc atgaaacaca ggaaaacata actaaacaac cagaacaaca agtgctgtcc
    15421 gattttacgt ctggtcagaa atgatataat tgtctccaac atcagagtaa aattacgaaa
    15481 atattagtca atacatcata ccctttatga acaatagacc aagtaaacca ttatttagat
    15541 gaaggtaatg atttggtttt tgaaaacatg gcttatattt gctttagtat tatttttaat
    15601 aaaatataaa tagaatgagt ttagaatctg aaacaccaat gaactctgaa gtacttcaga
    15661 tgaaggataa attcagagac atcattggtt tgcttggtga tgatgttaat agggaaggat
    15721 tgctcaaaac tcctgaaaga gtagcaaaag caatgaaatt tctaacaagt ggttatgaac
    15781 aggacccaag cgaaatacta aggtcggcac tgtttcatga agattataat gaaatggtca
    15841 tcgtgaaaga tatagaacta tattccatgt gtgagcatca catgttgcct tttttcggca
    15901 aagctcacgt ggcatacatc ccaaacggta aaatagtagg cctgagtaaa atacctaggg
    15961 tagtggatgt attttccaga aggttacaag tacaggaacg acttacacac gatattctta
    16021 actgtattga caatgtcctt aagcctcatg gtgctgccat tgtcatcgaa gctcaccaca
    16081 tgtgtatgat gatgcgcggt gtacagaagc aaaattctgt tactactaca tccgctttta
    16141 ccggagaatt taaaagaaat gaaacaagga acgaattcct gaaattaata gtagcaaaat
    16201 tggactgaat ccgtattttt ggtctttaaa cataaataaa ttacacagta tgagagcatt
    16261 tgtgtttccc gggcaggctt cacagtttgt cggtatgggc aaggatatgt acacgaacaa
    16321 tgaatctgca agaattttgt ttgaccaggc agacgaaatt ctcggttatc agatttcgaa
    16381 agtaatgttc gaaggtacag aagaggaatt aaaagaaacc agaataaccc aaccttctgt
    16441 ttttctgcat tcagttataa aagcgttgat taatcctgat gttgtacctg atatggtcgc
    16501 cggacactcg cttggcgaat tttcggcact cgtagtgaat ggttgtctcg acttcgcaag
    16561 cggattattg ttggtaaaag aacgtgcaga ggccatgcag caagcatgcg aacttgaaca
    16621 aagtaccatg gccgccatac ttggtctgga agataataaa gttgaagaaa tttgcaattc
    16681 tatcaatcat acggtcatag ctgctaatta taattgtccg ggccaattgg ttatctccgg
    16741 tagtattcaa ggtgtagagg aagcaatgaa agcctgtatg gaagctggtg ccaaacgagc
    16801 tatcaaatta gctgttggtg gagcattcca ctcgccattt atgcaacctg cacaagacag
    16861 actcaaagca gctatagaac aaacacagtt ctccgaacct ttctgtcctg tttatcaaaa
    16921 tgtcaatgca accccatcca ctgaccctga tgtcataaag aaaaatatcc tgatacagtt
    16981 aactgccccg gtattatgga ctcagattgt ccgcaatatg attactgatg gttgcgatca
    17041 ttttaccgaa gtaggtgggt cgggttcggt tctcagaggc atgatcagac agatagacag
    17101 aaatatatta tctgacgcct tataagatca gcattggaac atcaaaagat atcacaagga
    17161 agtttgcttc tcgctgaacc attcatgttg gatgtttatt tccgcagggc ggcaatactc
    17221 atttgtgata agcatgaaga gggcactctc ggatttatcc ttaacaagcc attggatgta
    17281 aaggttgata gtattataaa tgactttccg gagtttgatg cgcttgctta ctatggcgga
    17341 ccggtacaga cagattcggt acatttttta catactgccg gcgacatcat tgatgacagc
    17401 cagtttgttt gtgatggtat atactggggt ggtaattttg aaaaggtaaa agtcttgatt
    17461 actcagggtt tgttgcatcc gggtaatatc agattctatg taggatatac agggtggtca
    17521 aaatatcagc tcgaagcaga aattgaggat aaaacttgga taattgacga aatggatcct
    17581 aattatctct ttgttttcaa acccgataaa ctatggcagg taatccttga aaataaagga
    17641 aacacccatg cgattctcgg acagatagat gatttcctga acctcaacta aattttacgg
    17701 attgtcatcc ttggtttctg attgaatact ctcagtaacc ggtttgtgtt tgacaatatt
    17761 cagttttctt accacttcat cccgtacttt gaagaattct ggaagatcgg ccttgttcat
    17821 cacaggattg ctgctcacct tctcctttaa atgattgcat tgtacgccgt ttttccagaa
    17881 tctgaaacat acatgcggac ctgtggcaag gcctgtagaa ccgacatagc cgatggtttg
    17941 cccctgagat accctaacac cttttctgat gcctttcccg attcttgaca tgtgaagata
    18001 ctgtgtggta taggtggcat catgttttat tttaacataa ttgccattgt atccgtttct
    18061 gctagcttca attaccacac cattacctac agacataata ggtgttccgg caggtgcagc
    18121 ataatcagtc ccaaagtgag gtttgttata ttttaaaacc ggatgaaaac gtcttagact
    18181 gaaccccgaa gagatacggc taaatttgac aggagctttc aggaaatatc tcttcatagg
    18241 ccggccttcc agatcgtaat atccttcgcg gtcacctttc ttataatata tagcataata
    18301 gtctttgccg ttctgctcga aataagcacc gtacatttta cctacaccta cacttttgcc
    18361 atctatgctg tgatcttcat atagtacttt aaatttatca tctttctgaa cgtgatagaa
    18421 gtccacagta tatgcgaggg cgtcctccat tttgtcaatc aggtcataac tcagaccacc
    18481 ttctgccatg ctttcagaca aggtcgaatt cacgattcct tttgcaattt taatattcgt
    18541 ctgaatattt ttttgaatat agcttgcctg catagtatcc ctgaagtcaa gcttcacata
    18601 gccgtatgca tctggttcat atataaggta gtatggcttc tcggtcattt ttttgcacat
    18661 tacgctatat tctttccctg cacgaagttt ggttaggtca aatactttag ctgcctttgc
    18721 aaccatatta ttgatctcat tatcacttag ctcgtagttc ttcaggatat ctaccaaaaa
    18781 ttgatttggc ttgattaaag ttgttcttac ctccatagta tccagcaaaa gctcgtattt
    18841 gacattaggc atttcgagtg gaaatgcgct taattcactc aaagaagctt tcagactgaa
    18901 gtcggtggaa ctgtctttga ctgcataatt gaccaggtac atgagcagac ctgaaacaag
    18961 tgttatggat attggagccg gtattaatct tactttgttt aatgtttgac ttactatgtt
    19021 tgttaacttc accctctaca attttacttt atttaatttg gttttccggt catctctaaa
    19081 cgttagatga acaaaaatat tatttccttt tctaatcaca aaagctaatt taatattatt
    19141 ttcaatttta acattttaat atttttaata tgtattgaaa atatgctctt taacctgcat
    19201 gaactgtgcc aattttaccc attcatagac atcatttctt catagttatt catttctttt
    19261 tacctttaca gaaatttttc ctgatcacaa tgatttcacc ggttacaagt tttaattgca
    19321 gagggagact actggttttt gaggatccta ttatcatggg tattctcaac attacaccag
    19381 attcgtttta cgctgcaagt cgtgttactg gcacagatga gataatcgac aaggcaggac
    19441 agatgattga agatggtgca accattcttg acttgggtgc tatgtcttca agacctggag
    19501 cagtaaacat tgatacagat gctgaaattt cacgcttaat gccgttcctt tctgcagtca
    19561 gagatgcttt tccacaagta tttatttctg tcgatacttt tcgggctgag gtggccaagc
    19621 aggcatgctc ggctggtgct gacattatca acgatatctc aggtggtcga tttgatgaaa
    19681 atttgtttcg tgttattgca gatgcaaatg tgccttatgt gttaatgcac aacggtgcaa
    19741 ctttcgaatc aatgcattca aaaaaaggtc ttggcgatcc ggttgctact gtgtttgatt
    19801 tttttactga aaaaatacgc caactgaatg aatatggtat ttatgatatt tttctcgatc
    19861 cgggttttgg atttggaaag ctgatgcaag agaatttttc attattaaag agtatcggct
    19921 acttcaatat cctccatctg cccgtactgg ctggggtaag cagaaaaaag atggtttata
    19981 ccagtcttaa tatttcttct gatgatgcac tcaatggaac aactgcactt aatatgtatg
    20041 cgttggcaca aggttctaaa gtactccgcg tacatgatgt aaaagaagca tcacaatcaa
    20101 tagaattata taaacttatc cgtgattcgg ataaggaaat ttgtgctgaa tcaacattgt
    20161 atgaatagat taaaaacaaa atgtttatat tgttaaagag caaattgact ataattattt
    20221 aattattagt taattctctt ttatatttct ttatttgtat gtctataatc ataaattgtc
    20281 ggtcggattc gtttgaatga aaaaggtaaa ccttaaaaaa tatctcttta aaccatatca
    20341 cggtggcggc tttttacaaa agtttgcgct gtgggtgttc aggatatttg tactttttct
    20401 ttttctcaat ttttttcttt ttcttttttt tcaaatccca tctgtccaac gcaaagcaac
    20461 gaattacatt acaagttttt tagaatccag atctggtaca aaggtggagc tcgccagtgt
    20521 gcgtttcaat ttttttaata acttcgaatt gcgtggattc tcaatcgatg atctgcacgc
    20581 taaccctgtg atatatgctc aacagcttca gataaatttt agcgtcaatc cagttaactt
    20641 atacagacaa cgtattgtat tggatgaagt ggtactgaag aatgctggag taaatcttcg
    20701 gaaatatccg ggtgaaacag agcaaaacat tgatttgctg atacggaaaa tgttccctcc
    20761 taaggaggat aaaaagaaat cagacacgaa acttaagcta ataataaaaa caattcagct
    20821 ggcagtcgta gaatttacag actacaatga gcacaatggt aattacttaa gtataagtac
    20881 ccaacgtggg gaagcaaaag taggaaaaat ggactttgtc aataaagagt acatcttttc
    20941 aagtgtaata tttgatatgc cggtgttcag gctacaatac aggaaattat ccatggaagc
    21001 tattgaagca tccggtaagg taataaaaga aacagataac ccgaacggga aagttttgaa
    21061 acttgtcttt caggattttg aattaaatga cggttctctt tttgttgaca attaccagcg
    21121 gccacctgac ccaaatatta aaattagcgt tgacgacatt gactggtcgc attttgcttt
    21181 caatgatgta catgcaatcg gcaagaatct aatcatacac gatggttcgt acagtgcttc
    21241 accattgaag atttcactta aagaatcaaa aggttttgaa ataaaggagt tgacggccgg
    21301 gtttgcaggt attaataatg aacaaatacg gctggctgag ttaaatttac aaaccaaggc
    21361 aagtgtcatc agagattctg taatactcaa ctacgaaagc ctgtcatcat tgagtgatga
    21421 ctttgcaaac aatgtgggca tcaaaattgg gcttaataaa tcaatgattt ctgttagaga
    21481 aatagctcat tttgcgccct cattaaagta tgataaattt ttcgtcaata attttaatga
    21541 agtcctgact gtatctggtg aaatatacgg ccgtatcaat agtctgaaag ctaaaaacct
    21601 cattgtagaa ataccatcaa agctatacct ccatacagaa tttaagtcta atgatatttc
    21661 tcaaacagat aatgctatta tcaaccttga tataaagcag ctgaatagca atgtgcagtt
    21721 tctagaaaag gcaatcccca atcttgttct tccacctaat ttttcaaaac tgggagaatt
    21781 gtctttcaaa ggaaactttg acggcttctt cactgatttt gtcacctatg gaacattaaa
    21841 aaccaacctt ggcgatattg ggcttgacct taggatgaat ctgcagaatg gaaaggataa
    21901 ttcaatttat tctggtggaa tgaaactgaa gaattttaac ctcggtagct ggatagatca
    21961 aaataaattt ggcaatctgt cagcagattt caatgtcaga aacgggcacg gatttacagc
    22021 cgataaagct gaagctgacc ttgacggtaa ggtacattcc atagaataca atagttatac
    22081 ttatcatgat atagtgttga atggtatatt caaaaagaat acgatagatg gctcaattac
    22141 cggatccgat ccaaatgctg attttacctt tacaggaaag gtggatcttg gtgccgatat
    22201 tcccgagtat gatttccttt ctgagataaa aaatcttgat ctgtataagc taaacctttc
    22261 ttccaagcca ctcgctgttt ctggcaatct tgatataaac ctgcttacaa gaaattttaa
    22321 aacacttgat ggaacatcca aaattaacaa tctgagattt acaaatggtc aaaaaacctt
    22381 atttgagctt gatgaaattt tgctgaatgc cagccaaacc ggtaatgtca aaacgattga
    22441 tgtaaaatcg gatgtggcag atgtttacat atccggcgaa tttgaaattg ataatctaca
    22501 tcagatattt ttacaaaatc tgaagaaact ttatccgaga tattatgacg acattaagct
    22561 cccgaaagta aatttacctt ttacaaggac ccagtgcagc tacaaattcg acattaaaaa
    22621 aacggagcct ctgctggcat tgattgacaa tcctgtaagt tttgacggcc cattgttggt
    22681 gagtggcaaa tttaataact tgactaacca atggaatact ttagctacta ctgaaggtat
    22741 caaagtgggt gacaaagttt ttaaaggcat tactatcaat tatattcaat atgatgaaga
    22801 ggccaatttt tcgggaatac tcgacaaaac tgttgtaatg gataaagtac tcgataatac
    22861 aagatttact tttgtgataa accccgagaa tattaaattt gattttaatg gaataaaact
    22921 ttattcttct gacctgagtg tgaacggcac gctcaaacca acagatgatg gctacaatct
    22981 tcattttaat aataatagta tcaatctact cggcgaaaac tgggaagtca atcctgacaa
    23041 tctactaata atcaataaac agaccatcat acctgaatca tttgttctta ccaatgagaa
    23101 taggcaaata atactgaacc aaacaatcaa tgacggactt gagttcgcta ttattggata
    23161 tgatgtttcc aagctaaatg agatcattaa gtatgacaaa ctgaagcttt caggcaatct
    23221 tcaaatctat gcacgtgtca acgaagttta caagcttcgg aattttggac tccgtgtgta
    23281 cagcgattcg ctcaatatca ataaagacca ttggggaaaa ttattggtca atgcatcagc
    23341 aattgatttt aaagaactgc taaatgttga ataccaactt gaacacgaca caattgccat
    23401 gtctggaaga ggttttctca acataagtga ccgagagaac atcagactaa aactcgataa
    23461 tagatttaat tatttaccgc ttcgcacact tgaatatttt atcggcaatg gtatcaacaa
    23521 tacttcgggt tcgcttaagg gaaattttac tgttgaaggc ccgttgcata acatgggcct
    23581 atcgggtaag gcagacattg ccaaggctgg ctttcattt
//
LOCUS       DAOQQY010000013        23290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_655_length_23290_cov_6.712417, whole genome shotgun sequence.
ACCESSION   DAOQQY010000013 DAOQQY010000000
VERSION     DAOQQY010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23290
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_655_length_23290_cov_6.712417"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLE23_01520"
     CDS             <1..753
                     /locus_tag="PLE23_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81973.1"
                     /translation="FLNLQERSATDKLWRINSFEQVLNNFKAHYFASCILVPADSFLN
                     DLKRFIEKENFDSTFIEAWINKYQVSPELLMHRFNLLPQYLGLDKIFFLRVVHSVSAN
                     TFEIDKELHLSRRHDPQANRLKEHYCRRALSVSVFKKLKESDKNLVVDVQRSVYFNSS
                     NEYICLSVAQKYSDNPDKMISITAGLYLDEHSMSKLKFIHDSDIKRVVVNVTCERCSI
                     ADCQERAFDPTILIEKERRKKITKAIDDLLTL"
     gene            complement(748..2838)
                     /locus_tag="PLE23_01525"
     CDS             complement(748..2838)
                     /locus_tag="PLE23_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX81974.1"
                     /translation="MRKILSFIFLICFALSPVILSAQPANDDCYNPIIIKDVKNWCSQ
                     VGEFTNVGATPSSYGKPNCFTNVSHDVWFAFTALATDVVITITGKSSKNPGGTLLFPE
                     AALYNGYCGGTIGELECITDTKANSVIEMYQGGLFVGATYLIRVDGRATSEGTFQICI
                     NNYNPPADPQSDCSQAAILCDKTPFVVQSVQGAGKDTSELNGAPCFSGGASLNNEMNS
                     TWFTWKCQKAGSLTFTLKPNKIDDDLDFVLYELPNGLGNCTGKKIVRCMASGDNVYPS
                     KCMGPTGLREGETDVEEPAGCGLSSQNNFLAPLQMEVGKCYALVINNFTSTATGFSVE
                     MGGLGEFEGPTPGFTWDSDTVKCLEPINFIDQSSYSIGNITNWEWSFGEGALPQTAIG
                     QGPHNVVFQSYGIKYPTLTITTDLGCKVTLTKETYIDVCCLPGDNFEVGLDSIVEPSC
                     HGLSDGAMFFSATGGTPWYKYSIDGENFTGATAIGGLASGNYTVQAIDSHGCEDEIDV
                     KINQPPILLVDAGEDQTIDLGYGALITATTFPFDRPVAISWSSLNDTSAFCKTCFSFD
                     VIPPGTTTYVANIIDSTGCVASDEITIFVNDKKPIFVPNAISPNGDGYNDGFTLYGGV
                     AARKINLLRVYSRWGSLIFEAQDIDLNQPKLGWDGTFEGKICNPGVYVFYAEVSFVDN
                     KTKIFKGDINLLRL"
     gene            3149..3487
                     /locus_tag="PLE23_01530"
     CDS             3149..3487
                     /locus_tag="PLE23_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HMX81975.1"
                     /translation="MKKILFFALSLALFAACSKPASNLTYVSDTFTVNGSCGMCKTNI
                     EKAAKIPGVATAVWNQKANSLTVKYAPSKVDIMAVHKSIAAAGYDTDKAHGDDAAYGK
                     LPGCCKYRDK"
     gene            3675..4082
                     /locus_tag="PLE23_01535"
     CDS             3675..4082
                     /locus_tag="PLE23_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMX81976.1"
                     /translation="MRISIFRTAHFNAAHRLHVHHWSEEKNKATFGVCNNPNYHGHNY
                     ELEIKVTGELDDETGMLIDLNILKEMIEIHVLEKFDHKNLNLDLPEFKNMNPTAENIC
                     YVIWNIMRGLLDPQFDLHVRLYETARNYVEYPA"
     gene            complement(4177..6084)
                     /locus_tag="PLE23_01540"
     CDS             complement(4177..6084)
                     /locus_tag="PLE23_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HMX81977.1"
                     /translation="MKRIFTLLMFVLLCTLNTNAQTIYYLEDFENGMPADYKTYDLDG
                     LTPAAYPQFKGLGWIATGGIAASTSYYSPSGAANDWMVTKGISIPAASNPDNRVQLVW
                     FEQTTDASYPDGYEVYVSETGQAVADFETKLLTVTPAQTATTAATGTLRTIDLSAYAG
                     KTVYFAFRNNTFDGTVLIIDNITVVELGRYDMAAESVTNKIYNKSGNITIKGRWSNYG
                     WDQVKNFNFNYTVDGGAVTTANVGSATINPLDTKEYSASIPWASTAVGKHEIAIWPTN
                     INGQSDGEVLGDTSYLTVYVYDTDAEVTRNTLLETYTSSSCPPCKPGNEKIASVLNGV
                     NVQPLQVKFQQDFPGTGDPYCTAETVARRGYYGINAIPDTRIDGDFLALNPNGLVAKN
                     VTDAQNRAGLVNYDTKYKISADSQSITVFGTWSPTVDMMPGNVFHFAISEKQTNKNKT
                     SNGETVFYHVVKKMYPSLEGTNVDDISAGDVVSFEFTYTFPGKYRLPANGQAANVINL
                     ATEHSVENFNNLEVVMWIENTRDQYILNSGLAQFTTASNEISSIQKFVVYPNPSNEFA
                     NASINLNEALNGQLIVTDMAGRELWSAAQTLSAGNNTIELPIVSSLAAGTYNVTFKSG
                     YKVASQELIVK"
     gene            complement(6286..6954)
                     /locus_tag="PLE23_01545"
     CDS             complement(6286..6954)
                     /locus_tag="PLE23_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX81978.1"
                     /translation="MKKVLLALSLIFSGSYLFAQLQISPSKFDTVVDVSIFDIDLESH
                     VTNTGGSPIQVEWTRTVIDKAKAWGTYVCTGINCYPPNIESGEFDLAPGASDVFQMHF
                     TPANVSGYAIVEIKVVEKGNPSNFAIATYDVSGKAVATSSVRAEALRIYPNPTTEYFK
                     IQNNGSVAKVNILNLLGKNIKSYNGNTERFDVTDLPAGMYIVQMMDSKGKNIKTNKLS
                     IKKP"
     gene            7118..8464
                     /locus_tag="PLE23_01550"
     CDS             7118..8464
                     /locus_tag="PLE23_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX81979.1"
                     /translation="MHIAIIGNGISGTTAARFLRKYSHHRITMISAESEYFFARTGMM
                     YAFMGHLRFSDLLPYENWFWKKNKIDLVFDHVTRIDVLEKRLYLKRSPEISYDKLIIA
                     TGSKVNMPDIPGTSLHGVTGFYHKGDLENITNLHRSISNAVIIGGGLIGIELAEMFHS
                     KNIPVTMVVRDAGYWRNTLPYEESEMVGSLIKKQGIKLLLNTEVREIQGNAEGKVKSV
                     VLTDDNVIECDFVGIATGVLPQISLLNDTGIATEKGILVDEYLQTNNPDIFAAGDCAQ
                     IRSPDSGRKSIEPVWYTGRIMGELVANNICRNPVKYKPGIWFNSAKFFGMEYQVYGFV
                     PNKKSTEYDNVFWQHPDTQKSVRIMYEQSTEKVVGFGLMGIRYRHEVCEKWILEETQL
                     STVLADLSLANFDPEFFRQYEPLVLEQYFQQTGKKILPAKAKNLNAVIKFLKNVKR"
     gene            8461..10044
                     /locus_tag="PLE23_01555"
     CDS             8461..10044
                     /locus_tag="PLE23_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMX81980.1"
                     /translation="MKKIIKLLGVLIFLTGFVIFLLALREQRFTLMPHVVESVIVNPY
                     HKQLIKSVDYEMLEEQYESSEAFVNRLERLFRDVKIVEGLNTKNTSSEIQKNGAPLSE
                     YDINQYIFELTLASQLGGIIVESSWWLRMTFGLMMLGALLYIFTGLYESPESNNAGVW
                     QQSFQNRGLFGLLTGSFLIGFYIVLYWFPLYITSWIHLADPLSRSLNGNNAGQWFLYG
                     AMYTLAILVMGTRMLIRYRHDRYQIFRTISVMFFQTAFAFIIPEILSSLNKPRFDFKD
                     MWPLKYDFFFEWNLNTLLQSGHIGVFMLVWGIVLFLVAVPVFTWFFGKRWYCSWVCGC
                     GALAETAGDPFRQLSNKKLFAWRIERYLIYAILIFVIVMTLLVLYTFFTGSANIGPLN
                     SYDVRKWYGFLIGSVFAGVIGVGFYPLMGNRVWCRFGCPLAAYMGIIQKFRSRFRITV
                     NGDQCISCGNCSAYCEMGIDVRAYAQNGQDVVRASCVGCGICVAVCPRGVLSLENASE
                     DISQRGNIKRTIKLNLDDI"
     gene            complement(10091..10435)
                     /locus_tag="PLE23_01560"
     CDS             complement(10091..10435)
                     /locus_tag="PLE23_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81981.1"
                     /translation="MDSKEKLLQIQSELEVFKPLLSKATEMVLDEDISKYPIFVVHTD
                     TIEIGIPLVEREQSGKWSIHVSTLEEFAAKQLIQADKVEEFIKIYKNPTEAHCLFVLS
                     DLGARFIFIPSH"
     gene            complement(10523..12682)
                     /locus_tag="PLE23_01565"
     CDS             complement(10523..12682)
                     /locus_tag="PLE23_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX81982.1"
                     /translation="MKRIFTLSVLYFVVSIAFSQPAFQKSVLLQASVQNNPPSIQLKW
                     NKYTEATNYYIYRRTSIAAAWGSPLATVPPSDSTWTDTNVAVGKSYEYRVFRNGSAYP
                     ANGYLNAAINLPAKPNKGRIIVLADSVLVAKIEQEIARYLRDLTSEGWVPVYHIIPRE
                     MPVPDVKAIITAEYNIDPTNTKSLFLIGHIPVPYSGDLNPDGHPDHQGAWPADVYYAD
                     LNGTWTDASINDVSASDPRNRNIPEDGKFDQTIIPSPVELETGRVDFNNLPAFPDDEA
                     ELLRKYFDKNHAFRTKAFTAERRGVLEDNFGSFAEGFSQSGYKNFSSMFGINNVHVKP
                     YRNTLQNESYMWSYGCGGGNYVSASGINSTSNLVTDSLQTVFTMLFGSYFGDWDTKNN
                     FLRSALASGQTLTNAWSGRPIWQFHHMALGESIGYSAKLSMNNNGSTYEGGYGAQFVH
                     IALMGDPTLVQYTARPVQQLDITEQNGHILLSWSNYDDAHSGFELFKRYSSAEAFALV
                     SSLEADVTSYIDSCAIKGQNVEYCVRALIDEASASGTFQILSPGALNNITPTTESPAD
                     LSVSYSIANEHVTFTTTSNASSYHWDFGDGNTSTDKSPLHSYSKSGTYTVILTTTFEC
                     VSKKDTFSISVILTGETDLLTTTALQLYPTIANDQIQLHWQALSDVEYSIISTDGKLV
                     KNGILNPGLTILSVKEFNPGIYQIVTESRTLRFVIQR"
     gene            complement(12826..14103)
                     /gene="glyA"
                     /locus_tag="PLE23_01570"
     CDS             complement(12826..14103)
                     /gene="glyA"
                     /locus_tag="PLE23_01570"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMX81983.1"
                     /translation="MQHDYAIEQLLKDELNRQRHGLELIASENFTSLQVMQTMGSCFS
                     NKYAEGYPGKRYYGGCEVVDKAETLAIERLKKLFGAEYANVQPHSGAQANAAVFLGVL
                     KPGDPILGFDLSHGGHLSHGSPVNFSGKVYQANFYGVEEHSGLIDMDKVEQKAMEVKP
                     KLIICGASAYSRDWDYQRFRSIADKVGALLLADIAHPAGLIATQLLNDPLPHCHIITS
                     TTHKTLRGPRGGIIMMGKNFENPWGRTTKAGKPIMMSDILNSAVFPGMQGGPLEHIIA
                     AKAVAFHEALQDDYKDYTQRVVKNAQAMVKALMEKGYKIISDGTDNHLMLIDLRNKGV
                     TGKVAEQALVKAELTVNKNMVPFDDQSPMITSGIRIGTAAITTRGLDEKDCHQIVNWI
                     DQVITNVENDTTINKVKAEINEFMQQYPLYPEL"
     gene            14200..15741
                     /locus_tag="PLE23_01575"
     CDS             14200..15741
                     /locus_tag="PLE23_01575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HMX81984.1"
                     /translation="MRIKFFLFIVLSIILAKGLSAKSDKFRCMWRQDPATSMTIGWDQ
                     VSGTSPVVYFDTKDHGNDVAKYRYKKMPDTTRVFHEMRNTFVRFNNLVPNTVYYLIVK
                     DSEGQSRRLSFRTAPNDADTRLSIIAGGDSRNQREACRKANLLVSKLHPHFVLFGGDM
                     TDNDNAAQWKTWLEDWQLTITEDGRMTPIVVSRGNHEYSNKTLIELFDLPFTNLYYKL
                     AFGGNLLSVYTLNSLISAAGDQKDWLQEQLAADQDFIWRMTQYHFPIRPHVKIKTNKN
                     DQAIHWSNLFKTYDVGVAIESDAHLAKITYPIKPYSGPGSDEGFIRDDQGGTVYLGEG
                     GWGAPLRKNDSNKSWTMESGSFNHFNWLWVDRNDIQIRTVKTLNADQVVPLNINNVFA
                     IPEGVDLWSMAGNVVYTIDKKSRPSAETLLAARGDDASQTTLLKASADGKVRFGYTLD
                     AQANLEFSLYNNNHVEVLKTALPTQAPGKYERNFELKSLPPGKYLLIIRANKKLIQKY
                     KILKT"
     gene            complement(15738..16043)
                     /locus_tag="PLE23_01580"
     CDS             complement(15738..16043)
                     /locus_tag="PLE23_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="muconolactone Delta-isomerase family protein"
                     /protein_id="HMX81985.1"
                     /translation="MSQPTKFMVKFNLTENFTEAQYDLVPQQRMMVGEFFSEGKLASY
                     TLSLEYGKLWAVINAGSVMEVVELINKLPLTQYMQYKIYPLSFHQTMENSTMTFSLN"
     gene            complement(16230..17459)
                     /locus_tag="PLE23_01585"
     CDS             complement(16230..17459)
                     /locus_tag="PLE23_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMX81986.1"
                     /translation="MKKIKIFNDPVYGFITVPDNILMKIIDHPYFQRLRRIKQLGMTH
                     YVYPGALHTRFHHALGALHLMQQALEVLKMKGTDISEEESLATQIAILLHDIGHGPFS
                     HALEYILIDMHHEEISGLFFDQLNEEFDGQLSMAIEIFYNRYPKPFLHQLISGQLDMD
                     RMDYLNRDSFFTGVSEGVIGYDRIIKMLCVKDGNLTVEEKGIYSIEKFLMSRRLMYWQ
                     VYLHKTVLATEQMLISVIKRAKYLMTHYQENAVPSVVRGMFLAMISLSSRYPEDRKAF
                     LDVFSAWDDSDISFAIKSFMKSDDFVLSFLSSGLINRKLFKLEWKDEPFEEDYIAGKL
                     LSVCEYYGIKEEDADFLVLSGFEQNQAYTIEKDEILILTKEGLVKQVSQMDLGYNQDK
                     VKIRYYVCSPKIYLPDI"
     gene            17534..19093
                     /locus_tag="PLE23_01590"
     CDS             17534..19093
                     /locus_tag="PLE23_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HMX81987.1"
                     /translation="MEHIKILWADDEIDLLKPQQKFLEQKGYEVITVTNGHDAIEECN
                     EQRDFDIVFLDESMPGLSGLETLSRIKEVFAHLPVVMITKNEAETIMDEAIGSQITDY
                     LIKPVNPNQILLTLKKIIDNKRLVHTKTSSDYQQEFRQIFMEINSGLNFREWSEIYKK
                     LINWELKLDAANSVEMADILATQKSEANSEFTKYIIDHYEDWVNDKADEAPVMSNTLL
                     RTKVFPHLKDDVPTIVLLLDNLRFDQWKTIQPILTEQFRMEEEDTFFSILPTSTQYSR
                     NAIFSGMMPLEIEKRHPQWWKNDIDEGGKNLHEEDLLQELIKKSFRKEIKAEYVKVTN
                     VANAKTLQDNILNYLNNNLTVVVYNFIDMLSHARTEMEVLKELAGDETAYRSLTKSWF
                     VHSPLWAALQKLAGKDIQLFVTTDHGTIRVKNPARVVGDKETTTNLRYKVGRNLQYEK
                     KEVFELKDPKKGLLPTPNLSSTFIFAKNDLFFVYPNNYNYYQNYYRNTFQHGGVSLEE
                     IICPIVKLSGK"
     gene            complement(19098..19547)
                     /locus_tag="PLE23_01595"
     CDS             complement(19098..19547)
                     /locus_tag="PLE23_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81988.1"
                     /translation="MHQISSSLSLFWKIFLPIFTLVFLGLGMVIFWFGDVENNGPGQL
                     LVNRIIFTGLFFILAFVFYRTLIQIKRIDMDDQFVYVSNYFKTAKYTFDQIEKIEEKS
                     FLHRKIVSFYLNGKGAFGRKILFVPDRVRYEHFFKKHEVLSNLYTTV"
     gene            complement(19554..20381)
                     /gene="tatC"
                     /locus_tag="PLE23_01600"
     CDS             complement(19554..20381)
                     /gene="tatC"
                     /locus_tag="PLE23_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMX81989.1"
                     /translation="MALDQVDVDKLDESGKEMSFLDHLEALRWHIFRSAIAVCIAGIV
                     VFSYQDWVFANIILAPKNPDFITFKLLCRLGPTFCYQPPRFDLVTRELGEQFTQSMYV
                     GVWLGVILASPYLLYELWKFVKPGLYKKEQDAIRGIVFICTFLFLLGVAFGYFIMAPF
                     SIAFLASYSVGAVNMPTLSSYISYMTMFTLPIGLIFELPVVLYFLTKLGVITPNFLKN
                     YRRHAIVLIVILAGVITPPDVLSQLLVCFPLLLLYEISIVVSNKEYKRKQKLEAESE"
     gene            20591..20983
                     /locus_tag="PLE23_01605"
     CDS             20591..20983
                     /locus_tag="PLE23_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX81990.1"
                     /translation="MLKNILKKVLWAGAIIIALWLLVGSYFVFWGTYSDGYRIGTVIK
                     LNKKGYIFKTYEGQLNLDFFPPGGKGNTQSNLWDFSVADKSVADAIEKANDNGKKVKL
                     YFDEKYYRLPWVGETKYIVNRVEEINSN"
     gene            21001..21453
                     /locus_tag="PLE23_01610"
     CDS             21001..21453
                     /locus_tag="PLE23_01610"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HMX81991.1"
                     /translation="MVCLGNICRSPLAEGILAEEILHQDLDWSVDSAGTSGWHAGELP
                     DKRSIDVAKQHKLDITSQRSRKIRTTDIEHFDLILAMDKQNLQDLKAYFMKDPHLHYK
                     IKLISAFSNKFQDKDIPDPYYDNDGFEQVFEMLQDCIKGLLQTTHNIR"
     gene            complement(21534..21833)
                     /locus_tag="PLE23_01615"
     CDS             complement(21534..21833)
                     /locus_tag="PLE23_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HMX81992.1"
                     /translation="MNAFPQFDTQEDVLVETELETSHGKNAELIIYNDDYNTFDWVIH
                     CLMEICGHTYEQSEQLSLIIHFKGKATVKTAPKPELRPLKDGLIDRGLSAVIEEK"
     gene            complement(21867..22337)
                     /locus_tag="PLE23_01620"
     CDS             complement(21867..22337)
                     /locus_tag="PLE23_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMX81993.1"
                     /translation="MSLKPGDKAPLFKLFASDKSEVKLSSFKGRNVVLLFFPQAFTGT
                     CTTELCSVRDNMNIYSSLNAEVLAVSVDSVFTLARFKEEQGFNFPLLSDFNKKVSKAY
                     GAQYKDWILGMKGVAKRSAFVVDKKGIIQYAEVLESAGDLPDFDKIKQALDACQ"
     gene            22411..>23290
                     /locus_tag="PLE23_01625"
     CDS             22411..>23290
                     /locus_tag="PLE23_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMX81994.1"
                     /translation="MKRAKEKYHNLNNVTKAYLQLHLCVFLWGFTAILGKLISVAALS
                     LVWWRVAFTTLSLLFVKRLFSKIREIPRRKVGIYMFIGILVCTHWVLFYASIKASNAS
                     VGVLCMALSPIFTAFIEPLYFRRRILWFEILLSLVVIPGMYLVTQNISASMITGLVYG
                     IFSALFASWFSTLNKKYIEHSDPVAITFLELGSAWIFLSVIMLVSAAAGMDFSFIPKE
                     NDWLYLILLSVACTAIPYVLSLMSLKYISVFATNLTINLEPVYGIFLAWILLQEDKEV
                     DSGFYLGVLIILASVFS"
BASE COUNT         7063 a         4696 c         4600 g         6931 t
ORIGIN      
        1 tttcttaacc tacaggaaag gtcagcaaca gataagttat ggcgaatcaa ttcctttgaa
       61 caagttctga acaattttaa agcacattat tttgcttcat gcattcttgt acctgccgat
      121 tctttcctta atgacctgaa gcgctttata gaaaaagaaa attttgattc aacatttatc
      181 gaagcctgga tcaacaaata ccaggtatcg cctgagttgt tgatgcaccg tttcaatctt
      241 ttgccacaat atctcggact tgataaaata ttttttctta gagtagtaca cagcgtatcg
      301 gccaatacct ttgaaataga caaggagctt cacctgtcac gtaggcatga cccgcaggcc
      361 aacagattga aggaacatta ttgccgcagg gcactgtctg tttcggtttt caaaaaactc
      421 aaagaatctg acaaaaatct ggtagtggat gttcaacgat cagtctattt taattcgtcg
      481 aatgaatata tctgtctatc cgtggcacaa aaatattccg acaatccgga taaaatgatc
      541 agtattacag caggattata ccttgatgaa cactctatgt caaaactcaa attcatccac
      601 gattcagata ttaaacgtgt agtagtcaat gtcacatgcg aaagatgttc aatagcagac
      661 tgccaggaaa gggcatttga ccctactatt ctgatagaaa aggaacgaag aaagaagata
      721 acaaaagcta tagatgatct gcttacatta taatctgagc aaattgatgt ctcccttaaa
      781 tattttggtt ttgttatcca caaaacttac ctcggcatag aatacatata ctccgggatt
      841 acaaattttc ccctcaaagg taccatccca acctaatttt ggctggttga ggtcgatatc
      901 ttgcgcttcg aatatcaatg aaccccacct gctgtacact cgcagcagat tgatttttct
      961 ggcagcgaca ccaccgtata atgtgaagcc atcattataa ccatcaccat ttggtgaaat
     1021 agcattcggt acaaatattg gtttcttgtc gttgacaaat atggttattt catcactcgc
     1081 cacacacccg gttgaatcaa tgatattggc aacataggta gtagtacccg gcggaataac
     1141 gtcaaaacta aaacaggttt tacagaaggc tgaagtatca ttcaatgatg accagcttat
     1201 agccaccggc ctgtcaaatg gaaaggttgt agccgtaata agggcgccat atccaagatc
     1261 aatggtttgg tcctcaccgg catccacaag cagaatgggt ggctggttaa tttttacatc
     1321 tatttcatcc tcacaaccat gtgaatcaat tgcctgcaca gtataattgc cgcttgctag
     1381 tccgccgata gctgtggcac cggtaaaatt ttcaccgtct atactatatt tgtaccatgg
     1441 cgtaccaccg gtagcagaaa aaaacatagc accatctgac agaccatgac agctgggctc
     1501 tacgatacta tcaagcccta cttcaaaatt gtcgccaggc agacaacaaa catctatgta
     1561 cgtttcttta gtaagcgtca ccttacagcc taagtctgtg gtaatggtaa gggtcgggta
     1621 ttttatacca taggactgga acactacatt gtgaggcccc tgcccaatag cagtttgagg
     1681 taaagcacct tcgccaaacg accattccca gttggtaata tttccaatcg aataactcga
     1741 ctgatcaatg aaatttattg gctcaagaca tttaacagta tcagaatccc aggtaaatcc
     1801 gggagtaggg ccttcaaatt ctcctaaacc acccatctct actgaaaacc cagtggctgt
     1861 actggtaaag ttgttgatga ccaatgcata acactttcct acctccatct gtaaaggtgc
     1921 caggaaatta ttctgactgg aaagaccaca accagccggt tcttcaacgt ctgtttctcc
     1981 ctctcttaga cctgtcggcc ccatacattt gctcggataa acattgtctc ctgaagccat
     2041 acaccgtact attttcttac cggtacaatt accaaggcca ttgggtagct cgtagaggac
     2101 aaaatcaagg tcgtcatcaa ttttatttgg tttgagtgta aaagttagag atccggcctt
     2161 ttggcacttc cacgtaaacc acgtcgaatt catctcattg ttaagacttg cacctccact
     2221 gaagcacggc gcaccattta actcacttgt atctttgcct gccccctgta cactttgtac
     2281 cacaaaagga gtcttgtcgc ataggatggc agcctgcgag cagtcagact gtgggtcagc
     2341 aggtggatta taattgttga tacagatctg gaatgttcct tcagatgtcg ctcgtccgtc
     2401 aacacgtatc aagtatgtag cgccgacaaa aagacctcct tggtacattt ctataacgct
     2461 gttggcctta gtatctgtaa tacattcaag ctctccaatc gtacctccgc aatatccgtt
     2521 atacagagcg gcttcaggaa acagcaaggt gccaccaggg tttttggaag acttacctgt
     2581 aatggtaata actacgtcgg tggccagagc agtaaatgca aaccagacat catgtgacac
     2641 attggtaaaa caattaggct taccataaga ggatggtgta gctccgacat tcgtaaattc
     2701 acctacctgt gaacaccagt tcttgacatc cttaataata atgggattat agcagtcatc
     2761 atttgctggc tgcgctgata gaataacagg gcttaatgca aaacaaatta aaaatataaa
     2821 tgataaaatt tttctcatca aactgatact tgggtaatat gcaatgtaaa aataacgaaa
     2881 aaatccattt tgaagtgtaa aaagaaccgg ttaaatgcac aaagacaaat tgcttattaa
     2941 ctcaatacct attttaggct ttgaagcttg aatacactaa cgttatcgca tttgagaaat
     3001 gtattgttaa attttaatta aaccctaagt agaacttatc ttctgctatg aatttttgta
     3061 tttttatata ctaaaacaaa acttagaaat tccgaacaat ttatgagttt tcaggttgaa
     3121 taatataaac attaaaatca cattacttat gaaaaaaatc ttatttttcg cattgtcact
     3181 ggcattgttt gctgcatgtt caaagccggc atccaacctg acttatgtat ccgacacatt
     3241 tactgttaat ggcagctgtg gtatgtgcaa aacaaatatc gaaaaagctg caaagatacc
     3301 aggcgttgct acagcagtct ggaatcagaa agcaaattca ttaactgtta agtacgcacc
     3361 atctaaagtt gacatcatgg ctgtacacaa gagtattgct gctgccggat atgatacaga
     3421 caaagcacat ggtgacgatg cagcctatgg caaacttccc ggttgctgta aataccgaga
     3481 taaataatta tttaactact gtataaaagt gaggcagtaa ttttcagaaa catttcttaa
     3541 aaaattactg cctttgctta tttatgccga tagagttagt acttcatttt ttgcatctct
     3601 gtgcctgaca gaatatttaa tgtgattgta cttttggtta ttttactaat atattacctt
     3661 aaaaaataag caccatgcga atatcaatat tcagaacggc gcatttcaac gcagcacaca
     3721 gattgcatgt acaccattgg agcgaagaaa aaaacaaggc cacatttggt gtgtgtaata
     3781 accctaatta tcacgggcac aattatgagt tggaaataaa agtaacaggt gagcttgacg
     3841 acgaaacggg tatgctgata gacctgaata tactaaagga aatgattgag atacatgtac
     3901 ttgaaaaatt tgatcataaa aatctcaatc ttgatctgcc ggaatttaag aatatgaatc
     3961 ctacagccga aaatatttgc tacgtcatct ggaatatcat gcgtggctta ctagatcctc
     4021 agtttgacct gcatgtccgt ttgtacgaaa cagccagaaa ctatgtagaa tatccggcct
     4081 gacacacatt taatacataa caaatatcca aataaaaaag cttgactgaa aaacaacagt
     4141 caagcttttt tactataatt aggaaatcaa ggaatattac ttcacgatta attcttgtga
     4201 agcgactttg taacctgatt tgaaagtaac attgtaagta cctgcagcaa gtgagctcac
     4261 aataggaagt tcaattgtgt tgttacctgc gctaagagtt tgtgcagctg accaaagttc
     4321 tctacctgcc atgtctgtaa caatcaactg accattgaga gcttcattca agttgataga
     4381 agcgttagca aactcatttg atgggttagg ataaacaaca aacttctgaa ttgatgaaat
     4441 ttcatttgaa gctgtagtaa actgagccaa acctgagtta aggatgtact ggtcacgagt
     4501 gttttctatc cacattacta cctcaaggtt gttaaagttt tctacagagt gttctgtagc
     4561 aaggttgata acgttagctg cctgtccgtt agcaggaagg cggtacttgc ctgggaaagt
     4621 atatgtaaac tcaaatgata ctacgtcacc tgcactaata tcatcaacat ttgtaccttc
     4681 aaggctaggg tacattttct tcactacgtg atagaataca gtttcaccat tagatgtttt
     4741 attcttgttc gtctgttttt ctgatattgc aaaatggaaa acgttacctg gcatcatatc
     4801 aacagtaggt gaccatgttc caaatactgt tatactctgg ctgtctgcag agattttata
     4861 tttggtgtca tagttaacca aacctgcgcg gttctgtgca tcggttacgt ttttggcaac
     4921 aagaccgtta ggattaagag caaggaaatc tccatctatt cttgtatcag gaatagcatt
     4981 gataccataa taacctcttc tggcaacagt ttctgcagta caatatgggt cacctgttcc
     5041 agggaagtcc tgctggaatt taacctgtag tggctgtaca ttgacaccat tgagtacaga
     5101 tgcaattttt tcgttgcccg gtttacaagg agggcagctt gatgaagtat aagtttcgag
     5161 aagggtattt ctggttactt ctgcatctgt atcataaaca tacacagtaa ggtaagaagt
     5221 atcgccaaga acttcgccat cactctggcc gttgatgtta gtaggccaga tagcgatttc
     5281 gtgcttacct actgctgtac ttgcccaagg aattgatgca ctgtattctt tagtatccaa
     5341 tgggttaata gtagccgaac caacattagc ggttgtaacg gctccaccat cgacagtgta
     5401 gttaaaatta aagttcttta cctggtccca accgtaattg ctccaacgac ctttaatagt
     5461 aatgtttccg cttttgttgt atattttgtt ggtaacactt tctgcagcca tatcgtatct
     5521 gccaagttct actacagtaa tattgtcaat aataagtact gtaccatcga atgtgttgtt
     5581 tctgaatgca aaataaactg tcttacctgc ataagcactc aggtcaatag ttctaagtgt
     5641 acctgttgct gctgtagttg ccgtctgagc aggagtaact gtcaacaatt tggtttcaaa
     5701 atctgccact gcctggccgg tttctgatac ataaacttca taaccatcag ggtaactggc
     5761 atctgttgtt tgctcaaacc aaaccaactg tactcggttg tcaggatttg aagccgccgg
     5821 aatactgata cctttggtaa ccatccagtc gttggcagca cccgaagggc tgtagtaaga
     5881 tgtactagct gcaataccac cggtggctat ccaacccaat cctttgaatt gcggataagc
     5941 tgccggtgtt agtccgtcaa gatcataggt cttgtaatcg gcaggcatgc cgttttcaaa
     6001 atcttctagg taataaatgg tctgtgcatt tgtgttaagc gtacacaata gcacaaacat
     6061 taaaagtgta aaaatgcgtt tcattgatat tgagtttaat taaaaataaa aaatgaggtg
     6121 caaaattaat atattagatt gatgttcttt tgattataca agaaatttaa catcaaaatg
     6181 ttcaaaaaaa tacttactta atatataaaa ataataaaaa ggctacggta tgattcttaa
     6241 ccgaagcctt tttataaaac aataattcta atatttgtaa gagcattaag gttttttaat
     6301 gcttagtttg tttgttttga tattcttacc ttttgaatcc atcatttgta cgatgtacat
     6361 acctgcaggt agatccgtca cgtcaaatct ttctgtattg ccattgtatg acttgatgtt
     6421 tttgcccaaa aggtttagaa tgttcacttt agcaaccgaa ccattgttct gtattttgaa
     6481 gtattcagtt gtagggtttg gatatattct caatgcttct gccctaacac tcgaagtagc
     6541 aactgctttg cctgagacat cataagtagc aatagcaaaa ttagacggat tgcctttttc
     6601 tacgactttt atttctacaa tcgcataccc tgaaacattg gccggggtga aatgcatttg
     6661 gaacacatct gatgctccgg gtgcaaggtc aaactcacct gattcaatat ttggcgggta
     6721 acaattgata cctgtacata cataagtacc ccaagctttt gccttatcaa taacggtacg
     6781 agtccattcg acctgaatag gcgatccacc agtattggtc acatgcgact caaggtcaat
     6841 gtcaaatatc gatacatcta caaccgtatc aaatttggaa gggcttatct gcaattgtgc
     6901 aaataaataa gaaccggaga aaattaaact taacgcaagt agaacttttt tcataattgg
     6961 atttgattag tcccccaaaa ttacacagct atttggaatt aatcaaatta aaagaaaata
     7021 tttaggaaat tttaaccttt ttatacggct tatttacctg tttttctcgt attaacttaa
     7081 tcatcaatga aattttacat tttttgaaca cctgtctatg catatcgcaa ttatcggtaa
     7141 tggtataagt ggcaccacgg cagccagatt tctcaggaaa tattcacatc acagaattac
     7201 catgatttct gctgagtcag aatacttttt tgcccgaacc ggtatgatgt atgctttcat
     7261 gggccaccta cgcttctccg atctgttgcc ttacgaaaac tggttttgga aaaagaacaa
     7321 aatagatctg gtatttgacc atgtgaccag aatcgatgtg cttgaaaaac gactgtacct
     7381 taaaagatca cctgaaattt cttatgacaa acttataatt gccaccggct caaaagtaaa
     7441 tatgcctgat atacccggaa cttcgctgca tggtgttact ggattttatc ataaaggcga
     7501 tcttgaaaat attacaaatc tccacagaag tatcagtaat gccgtaatca tcggtggtgg
     7561 tctcattggc attgaactgg cagaaatgtt ccattcaaaa aatataccgg tgactatggt
     7621 agtgcgcgat gctggttatt ggagaaacac acttccgtat gaagaatctg aaatggttgg
     7681 cagcttgatc aaaaaacaag gaataaagct cttgctgaat accgaagtca gagaaataca
     7741 aggcaatgca gaaggaaaag tcaaaagtgt tgtacttaca gatgacaatg ttattgaatg
     7801 tgattttgta ggtattgcca ccggtgtact tcctcaaata tccttgctga atgataccgg
     7861 aatagcaacc gaaaaaggta tcctggtaga tgaatatctc caaacgaaca atcccgacat
     7921 atttgccgcc ggtgattgtg cacaaatacg ctcacctgac tccggaagga aaagtataga
     7981 accggtctgg tacacaggaa gaataatggg agaactggta gctaacaata tttgccgaaa
     8041 tcctgtaaag tataagcctg gcatctggtt caattctgca aaatttttcg ggatggaata
     8101 tcaggtgtac ggctttgtac ctaacaaaaa aagtactgag tatgacaacg tcttctggca
     8161 gcatcctgat actcaaaaat ctgttcgtat tatgtacgaa caaagcactg aaaaagtcgt
     8221 cggatttggg ctcatgggta tccgttaccg acacgaagta tgcgaaaaat ggatacttga
     8281 agaaacacag ctctctaccg ttcttgcaga tctttcatta gccaatttcg atcctgaatt
     8341 tttcagacaa tacgaaccct tggttttgga acaatatttt cagcaaacag gaaagaaaat
     8401 ccttccggca aaagccaaaa accttaatgc tgtcatcaaa tttcttaaaa atgtaaagag
     8461 gtgaagaaaa taataaaatt attaggtgtt ttgatttttc tcacaggatt tgtcattttc
     8521 ctgctggcgc ttcgcgaaca gagatttacc ctgatgccac atgtagtaga gtctgttatt
     8581 gtcaaccctt accataaaca actgataaaa agtgtcgatt atgaaatgct tgaagagcaa
     8641 tacgaatcaa gtgaagcatt cgtaaaccgg cttgaacgac tgttcaggga tgttaaaatt
     8701 gttgaaggcc tgaatacaaa aaatacatca tcagagatcc aaaaaaatgg tgctccactt
     8761 agcgagtatg atatcaacca atacatcttc gaacttacac tggcatcaca gttaggtggc
     8821 attattgtag agagcagttg gtggctacga atgacattcg ggttaatgat gctcggagcc
     8881 ttgctgtata tcttcaccgg tctgtatgaa agcccggaat ctaacaacgc aggcgtttgg
     8941 caacaatctt ttcaaaaccg tggactattc ggattgttga caggtagttt tctgattggg
     9001 ttttatattg tactttactg gtttccttta tacatcacca gctggataca cttggcagac
     9061 cctttgagcc gaagcctaaa tggcaataat gccggtcaat ggtttttata tggcgccatg
     9121 tacacactcg ccattttggt catgggcaca cgaatgctca taagataccg acatgacaga
     9181 tatcagatat tccggactat ttcggtcatg ttttttcaaa ctgcctttgc ttttattata
     9241 cctgagatat tgtcaagcct caacaaaccg agattcgact ttaaagatat gtggcctctg
     9301 aagtatgatt ttttctttga atggaatctg aacaccttac tacagagtgg gcatatcggt
     9361 gtatttatgt tagtatgggg cattgtacta ttccttgtag ccgtaccggt gttcacttgg
     9421 ttctttggca aacgatggta ctgttcgtgg gtatgtggct gtggtgcact tgcagagaca
     9481 gcaggtgacc ctttcaggca attgtccaat aaaaagctgt ttgcctggcg aatagagcgt
     9541 tatcttattt atgctattct tatttttgtc attgtcatga ccttactggt tctgtacaca
     9601 ttttttacag gatcggctaa tattggccca ctcaattctt atgatgtccg taagtggtat
     9661 ggattcctta ttggttctgt atttgccgga gttattggtg ttggttttta tccactgatg
     9721 ggtaatagag tgtggtgtcg gtttggttgt ccgttggctg cttatatggg tatcattcag
     9781 aagtttaggt caaggttccg gataacggta aacggtgacc aatgcatttc atgtggcaat
     9841 tgttccgctt attgtgagat gggcattgat gtacgtgctt acgcacaaaa tggccaggat
     9901 gtagtgaggg cttcttgtgt aggctgtggt atatgtgtgg ctgtctgccc aagaggcgtt
     9961 ctcagcctgg agaatgcatc agaagatatt tcacaaagag gaaatattaa aagaaccata
    10021 aagctcaatc ttgatgacat ttaacccatt gcaggcaaac cgggctattg aaaaaaccat
    10081 agctcgtcta tcaatgtgaa ggaataaaaa taaaccgtgc cccaaggtcg ctcaacacaa
    10141 aaagacaatg agcctctgtt gggtttttgt atatttttat aaattcttct accttatctg
    10201 cctgaataag ttgtttggca gcaaattctt cgagagttga tacatgtata gaccacttgc
    10261 cagactgttc gcgttctacc aaaggaatgc ctatttcgat ggtatcggta tgtaccacaa
    10321 aaatcgggta tttcgaaata tcttcatcca gcaccatttc ggttgccttt gacagtaatg
    10381 gtttgaatac ttcgagttcg gactgtattt gcaataattt ttctttcgaa tccattcgtt
    10441 ttatttcgtt tacctaaggg cacaaaagta ctgaataaac cacaagctgt agtgtttgtt
    10501 tgtagtaaaa tacagcaatt ctttatcgtt gtattacaaa tctcagtgtt ctgctttcag
    10561 ttacaatttg gtatatgcca ggattaaatt ctttgacgct aagtatagtt agccccggat
    10621 tgagaatgcc gttttttacc aacttgccat ccgtagaaat gatactatat tctacatccg
    10681 acaatgcctg ccaatggagt tgaatctggt cattggctat tgtaggataa agttggagcg
    10741 ccgttgttgt taagaggtcg gtttcgccag taagaattac actgatactg aatgtgtctt
    10801 ttttagacac acactcgaaa gttgtggtca gtattactgt ataagtacct gacttggagt
    10861 atgaatgtaa aggtgatttg tcagtcgagg tatttccatc accaaaatcc caatgataac
    10921 tggaagcatt gcttgtagtt gtaaaagtaa catgctcatt ggcaatagaa tagcttacgc
    10981 ttaaatcggc cgggctctct gtcgtaggtg taatattatt taatgcacct gggctgagta
    11041 tctggaaagt accactggca cttgcctcgt caatgagagc acgcacacaa tactcaacat
    11101 tctgtccctt tatggcacaa gaatctatgt atgatgtaac atcagcttcg agactgctga
    11161 ccagtgcaaa tgcttcagct gaagaataac gtttgaatag ttcaaaaccc gaatgtgcat
    11221 catcgtaatt gctccagctt aacagaatat gaccattttg ctcagtaatg tccaactgtt
    11281 gaacaggtcg tgcagtatat tgtaccaatg taggatcgcc catgagtgct atgtgtacaa
    11341 actgtgcacc ataaccacct tcataggtag agccattatt gttcatgcta agttttgcag
    11401 agtagccaat actttcgccc aatgccatgt ggtgaaactg ccaaatcggc ctgcccgacc
    11461 atgcattggt tagtgtctgg ccactggcca atgcagaacg aagaaaatta ttcttagtat
    11521 cccagtcgcc gaaataacta ccgaagagca ttgtgaaaac tgtctgcaga ctatccgtta
    11581 ccagattgct ggtagagtta atgccggaag cagaaacgta attgccaccg ccacagccat
    11641 agctccacat atagctttcg ttctgaagtg tgtttctgta tggtttgaca tgcacattat
    11701 ttataccaaa catgcttgaa aaatttttat aaccactctg agaaaaacct tcagcaaaac
    11761 ttccgaaatt atcctcaagt actcctcgtc tttcggccgt gaatgccttg gtacgaaatg
    11821 catgattttt gtcgaaatat tttctgagca actcagcctc atcatcagga aatgcaggga
    11881 gattgttaaa gtcaaccctt ccggtttcta gttcgaccgg agacgggatg atggtttgat
    11941 caaatttgcc atcttcaggt atattcctgt tccttggatc ggatgcactt acatcattta
    12001 ttgatgcgtc agtccaggta ccattcagat ctgcataata gacatcggca ggccatgcac
    12061 cctggtggtc cgggtgtccg tctgggttga ggtctccact ataaggtacc ggtatgtgcc
    12121 cgattaggaa aagagatttt gtgttggttg ggtcaatatt atattctgct gttataatcg
    12181 cctttacatc aggtacaggc atttctcgcg gtattatgtg ataaacaggt acccagcctt
    12241 cacttgttaa atcgcgaaga tatctggcta tttcctgttc aatctttgca accagtacag
    12301 aatcagccag aacgataatt ctccctttat tgggctttgc cggcaggttg atagcagcat
    12361 ttagataacc attggcaggg tatgcactac cgtttctaaa aacacggtat tcataagatt
    12421 tacctactgc cacatttgta tctgtccagg ttgaatcaga aggtggcact gtagccaaag
    12481 gtgagcccca tgcagctgca atgcttgtac ggcgatatat ataatagttg gttgcttcag
    12541 tatatttatt ccatttaagt tgaatagacg gagggttatt ttgtacgctt gcctgcagta
    12601 atacactttt ttgaaatgcg ggttgagaaa atgctatact tacaacaaaa tacaacacag
    12661 ataaggtaaa gattcttttc ataagtcgca atttttcatt tattaaatta aagataagtt
    12721 ggtttcaaaa atcgttgccg acagtatcaa aataacaata ttcaagcata aaaaagacat
    12781 attttaacat aatataatcg caccgatagt aagacttatt tataattaaa gctcagggta
    12841 taatgggtat tgttgcataa attcgttgat ttcagctttt accttgttga tggttgtatc
    12901 attttcaacg ttggtaatta cttgatcgat ccaatttact atctgatgac aatctttttc
    12961 atcaaggcca cgggtggtaa tggctgcagt gccgattctt ataccggagg taatcatagg
    13021 tgactggtca tcaaatggta ccatgttttt attcacagtg agttctgcct ttaccaatgc
    13081 ctgttctgcc actttgcctg ttacaccttt atttctaaga tctatcagca tgaggtggtt
    13141 atcggtgcca tccgatatta ttttataacc tttttccatt agtgccttta ccatagcctg
    13201 tgcatttttt acaacacgct gtgtataatc tttataatca tcctgcaatg cttcatggaa
    13261 tgcaactgct ttggctgcaa tgatgtgttc gagtgggcct ccctgcatac ctgggaatac
    13321 ggcactgttg aggatgtccg acatcatgat tggtttaccg gctttggtag tacgccccca
    13381 tggattttca aaatttttgc ccatcatgat gatcccgcct cttgggcccc ggagtgtttt
    13441 gtgtgtggta gaggtaatga tatgacaatg tggcaacgga tcgtttagta gctgggtagc
    13501 tataagaccc gccgggtgtg ctatgtcagc caacaacagc gcaccaacct tatcagctat
    13561 agacctgaac cgctggtagt cccaatcgcg tgaataagca gatgccccgc agatgatgag
    13621 ctttggcttt acctccattg ccttttgctc tactttatcc atatcgatga gtccgctgtg
    13681 ttcttctacg ccatagaaat ttgcctgata gacttttccg gaaaaattga ccggagaacc
    13741 atgagaaagg tgcccaccgt gcgaaaggtc gaagccgaga attgggtcgc ccggcttaag
    13801 tactcccaaa aagacagctg cattggcttg agcacctgaa tgtggctgta cattggcata
    13861 ttctgccccg aataactttt ttagcctttc gatggcaagg gtttctgcct tatcaactac
    13921 ttcacaacca ccataatatc gtttgccggg gtatccttca gcatatttat tagaaaaaca
    13981 cgaacccata gtctgcataa cctgcagact ggtaaaattt tctgaagcta tgagttccag
    14041 gccatgcctt tgtctgttga gttcatcttt gagtaattgt tctatcgcgt agtcgtgttg
    14101 cataagtcgt atgttgatat gttaataaat ttaaaattag gcatcaaatt tagactttta
    14161 tcagtaatgt ttacgttatt tattttatat ttactttgca tgcggataaa attttttttg
    14221 tttattgttt tgtcgattat tttagccaag ggactctcag ccaagagtga taagttcagg
    14281 tgtatgtggc gacaggatcc ggcaacttca atgaccatag gttgggacca ggtcagcggc
    14341 acttcgcctg ttgtatattt tgatactaaa gaccatggaa atgatgtggc aaaatacagg
    14401 tacaaaaaaa tgccggatac cacacgtgtg ttccatgaaa tgcgcaatac gttcgtaagg
    14461 tttaataatc tagtacccaa tacagtctat tatttgatag taaaagacag cgaaggacaa
    14521 tcacgacgtt tatcgttccg cactgccccc aatgatgctg atacaaggct tagtatcata
    14581 gcaggaggtg acagccgcaa tcagcgtgaa gcatgccgca aggctaatct gctggtttca
    14641 aaattgcatc cgcattttgt gctatttggc ggcgatatga ccgacaatga caatgcagca
    14701 caatggaaaa catggctcga agactggcaa ctcaccatta cggaggatgg gcgtatgaca
    14761 ccgatagttg tatcaagggg caatcacgaa tattccaaca agacactgat cgaattattc
    14821 gacctgccat ttaccaatct ttactataag cttgcattcg ggggcaatct actgagtgtc
    14881 tatacactca actcactgat atcggctgcc ggagaccaaa aagactggct acaagaacaa
    14941 ctggctgctg atcaagattt tatctggcga atgacacagt atcatttccc gataaggcca
    15001 catgtaaaaa tcaagactaa taaaaacgat caggctattc attggtccaa tctgtttaag
    15061 acatacgatg taggtgtagc catcgagtct gatgcccatc ttgccaaaat cacctaccct
    15121 atcaaaccat acagcggacc cggctccgat gaaggattta taagagacga ccagggtggt
    15181 accgtctatc tcggcgaagg tggatggggc gccccgctcc gcaaaaatga cagcaacaag
    15241 tcttggacaa tggaaagtgg aagctttaac cattttaact ggttatgggt tgatagaaac
    15301 gatatacaga tccgcacagt aaaaaccttg aatgctgatc aggttgttcc tcttaacatt
    15361 aacaatgttt ttgcaattcc ggagggcgtc gacctatgga gtatggccgg aaatgtggtt
    15421 tatacaatag ataaaaaatc aagaccatca gctgagactc tccttgcagc acgaggtgat
    15481 gatgcttcac aaaccacatt gctcaaagct tctgctgatg gcaaggtacg ttttggatat
    15541 acacttgacg ctcaggcgaa tctcgaattt tcgctataca acaacaatca tgttgaagtg
    15601 ttaaaaacgg cattacccac acaagcaccc ggcaagtacg aacgcaattt tgagctaaag
    15661 agcctaccac ccggcaaata tctgctcata atacgagcta ataaaaagtt gattcagaaa
    15721 tacaaaatcc tgaaaactta attaagtgaa aaagtcatag ttgagttttc cattgtctga
    15781 tgaaagctga gtggataaat cttgtactgc atgtattgtg tgagcggaag tttattgatg
    15841 agttctacta cttccattac actgcctgca ttgattactg cccaaagttt gccgtactcg
    15901 agcgataatg tgtaggaggc aagttttcct tcagagaaaa attcgccaac catcatcctt
    15961 tgctgaggca caaggtcata ctgagcttca gtaaagtttt ccgtaagatt aaatttgacc
    16021 ataaattttg taggttggct catgttttga atttgctttt ttaacaatga agtaaagtaa
    16081 atgttttgac ataaaaattc tgcaaaagta ttaggcaaca tctgatattt attcttatgg
    16141 gttgttgcac acaatatccg gaagtcctca atggaaatca accacggttc gatgtttatt
    16201 cttcagcaaa aataaagtat caaaataaat tatatgtcag gtaaatatat tttcggactg
    16261 cacacataat accttatctt taccttgtcc tgattatatc cgagatccat ttgcgaaacc
    16321 tgctttacca gaccttcttt tgtaagtatc aggatttcat ctttttcaat ggtgtaggct
    16381 tggttttgtt caaatccact cagaaccaaa aaatcggcat cttcctcttt tatgccataa
    16441 tattcacaca ctgaaagcaa cttaccggca atatagtctt cctcaaatgg ttcatccttc
    16501 cattcgagtt tgaaaagctt acgattgatg agtccggatg aaagaaagct caggacaaaa
    16561 tcatccgatt tcataaaact cttgatggca aatgaaatat cgctgtcgtc ccaggccgaa
    16621 aaaacatcaa gaaatgcctt cctgtcttcc ggataacggc tgctgagcga aatcatggcc
    16681 aaaaacatac cacgcactac agaaggcact gcattttctt gatagtgtgt catcaggtat
    16741 ttagcacgct ttatcacaga tatgagcatt tgctcagtgg caagtactgt tttgtgcaga
    16801 tatacttgcc agtacatgag ccgacgcgac atcagaaatt tttcgatcga gtatattcct
    16861 ttttcttcta ctgtaagatt tccatctttg acacaaagca ttttgataat ccggtcgtag
    16921 ccaattaccc cttcacttac acctgtaaaa aaactgtcgc ggttgaggta atccatcctg
    16981 tccatatcga gctgtccgga tataagctgg tgcaagaatg gcttcgggta acggttgtag
    17041 aatatttcta ttgccatact gagctggccg tcaaattctt cattgagctg gtcgaagaac
    17101 aatcccgaaa tttcttcgtg gtgcatgtca ataagtatat actcgagtgc atgcgaaaac
    17161 ggcccgtgcc ctatatcgtg caacaaaatg gctatttgag tagcaagcga ttcttcttct
    17221 gatatatctg ttcccttcat tttcaacacc tcaagtgcct gctgcatcag gtgtaatgct
    17281 ccgagtgcat ggtgaaagcg tgtatgcaac gcacccggat aaacatagtg tgtcataccg
    17341 agttgtttga tacgacgcaa tctctgaaaa taaggatgat caataatttt catcaaaata
    17401 ttgtccggca cagtaataaa accgtaaaca ggatcattga atatttttat ttttttcatt
    17461 gattcaaatg tatgaataat attgcacgta atcaatacgt ttatatccaa cgataaaatt
    17521 aagcataaat aacatggaac atataaaaat tctctgggct gatgatgaaa ttgatttgtt
    17581 aaaaccacaa caaaagtttc ttgaacagaa aggctatgaa gtaatcactg tcaccaacgg
    17641 acatgatgca atagaagaat gcaacgaaca gagggatttt gacattgttt tccttgacga
    17701 atcaatgccg ggcctttctg gtctcgaaac cctttcacga atcaaggagg ttttcgccca
    17761 tctaccggta gtgatgatta ccaaaaatga ggcagaaacc ataatggatg aagctatcgg
    17821 atcgcagata accgactatc tgattaaacc ggtaaaccct aaccagatat tgctgacgct
    17881 taaaaaaatc atcgacaaca aacgtctggt gcataccaaa acttcatcag actaccagca
    17941 agagtttcgt cagatattta tggaaataaa cagtggactc aacttccgtg aatggtcaga
    18001 aatttacaaa aaactcatca actgggagct caagctggat gctgccaata gtgttgagat
    18061 ggccgatata cttgctaccc aaaagtcgga agccaactct gagtttacga aatacataat
    18121 agaccattat gaagactggg tcaatgataa ggcagatgaa gcaccggtta tgtccaatac
    18181 ccttttacgt acaaaggtat ttcctcactt gaaggacgat gtacctacca tcgtactttt
    18241 gcttgataat cttaggttcg atcagtggaa aaccatccaa cctatactta ccgaacaatt
    18301 ccgaatggaa gaagaggata cttttttcag cattctgcct acatctacac aatacagtcg
    18361 caatgccatt ttctcaggaa tgatgcctct cgaaatcgaa aaacggcacc ctcaatggtg
    18421 gaaaaacgac attgacgaag gcggcaaaaa cttgcatgaa gaagacctgc tccaagaact
    18481 catcaagaag agcttcagga aggagataaa agcagaatat gtaaaggtaa caaatgttgc
    18541 caatgccaaa accctacagg acaacatact caactacctg aacaataatc tgacggtggt
    18601 agtctataac tttatcgata tgctaagcca tgctcgtact gagatggaag tactcaagga
    18661 gcttgcaggt gatgaaacag cttaccgatc gcttaccaaa tcttggtttg tgcactcgcc
    18721 actgtgggca gctttacaaa agcttgcagg caaagacatc cagctgtttg taactaccga
    18781 ccacggaacc atacgggtca agaatccggc aagggtagta ggtgacaaag aaacaaccac
    18841 caacctgcgc tacaaggtag gtcgcaatct ccagtatgag aaaaaagaag ttttcgaatt
    18901 gaaagacccg aaaaaaggat tactaccgac tcctaacctg agttctacct tcatttttgc
    18961 aaagaatgat ttgttttttg tgtatcctaa taattacaat tactatcaga attattacag
    19021 gaatacattt caacatggtg gtgtatcact cgaagagata atatgcccca tagtgaaatt
    19081 atcaggcaaa taatacgcta aactgttgta taaagattag agagtacttc gtgttttttg
    19141 aaaaaatgtt cataacgcac gcggtctggt acgaataata ttttacggcc aaaagcacct
    19201 ttgccattta agtagaaact tacaattttt cggtgcagaa acgatttttc ttcaattttt
    19261 tctatctgat cgaatgtgta cttggctgtt ttgaaataat ttgacacata aacaaactga
    19321 tcatccatgt ctatccgctt tatctgtatt aatgtacggt aaaacacaaa tgccaatatg
    19381 aaaaataagc cggtaaaaat gatccggtta actaataatt gcccggggcc attgttttct
    19441 acatctccaa accagaatat aaccatccca agtccaagaa aaacaagtgt aaaaataggt
    19501 aggaagattt tccagaagag gctcagtgaa cttgatattt gatgcataaa aatttattcg
    19561 ctttcagctt cgagtttttg ttttcgttta tattctttgt tactcacaac tatacttatt
    19621 tcatacaaca gtaacagcgg gaagcacact agtaactggc tcaatacgtc aggtggagta
    19681 ataacacccg caagtattac tatgagtacg atggcgtgac ggcggtagtt cttcagaaaa
    19741 ttgggcgtaa tgactccgag ttttgtaagg aaatatagca ccacaggcag ctcaaatata
    19801 agaccgatgg gcaatgtaaa catcgtcata tagctgatat aagaagatag tgtgggcata
    19861 tttaccgcac caaccgaata acttgcaaga aatgcaatac taaaaggtgc catgataaaa
    19921 tatccgaatg ctacaccaag tagaaataaa aaagtacaga taaacacaat cccgcgaatg
    19981 gcatcctgct ccttcttgta caatccgggt tttacaaatt tccacaactc atacaacaga
    20041 tatggcgaag ccagaatgac tcccagccat acacctacat acatactttg tgtaaactgt
    20101 tcacccaact cacgtgtcac gagatcaaac cgaggtggtt ggtagcaaaa agtaggacct
    20161 aacctacaca gtagcttaaa tgttataaaa tcagggtttt taggtgcaag aataatattg
    20221 gcaaacaccc agtcttgata agaaaaaaca actattccag caatacagac agcaattgca
    20281 gagcggaaaa tatgccatct caatgcttcg agatgatcaa gaaaactcat ttcttttcct
    20341 gactcatcca acttgtcaac atctacctga tctaaagcca tatttcactt aaaagcgcaa
    20401 agttaatatc tcagttggta aggcagtaga aaacatctga ttattaacat gcccaaagta
    20461 cttttatata aacattttaa tgttattaag tctgtatcta gtacaagtga taaaatattt
    20521 ttaaattttt gggaacgaaa aagaaaaaaa tcatgtttaa aatatcaatt ttgtatcaaa
    20581 tacaatacag atgttaaaga atatacttaa aaaagtgctc tgggccggtg ctatcatcat
    20641 cgctctgtgg ctgttggtag gctcctattt tgtattttgg ggcacctact ctgacgggta
    20701 caggataggc acagtcatca aactcaataa aaagggctat atctttaaaa catacgaagg
    20761 ccaactcaat ctcgattttt tcccgcctgg aggcaaagga aatacacaat ccaacctctg
    20821 ggatttctct gtggcagaca aatcggtagc agatgcaatc gaaaaagcca acgataacgg
    20881 caaaaaggtg aagctatatt ttgatgaaaa atattacaga cttccctggg tcggcgaaac
    20941 caaatatata gtcaaccggg tagaagaaat taatagcaat tagaacacgt acgtatatta
    21001 atggtatgtc ttggcaacat ctgccgctca cctttggccg aaggaatcct tgctgaagaa
    21061 atacttcacc aagacttgga ctggagtgtt gacagtgcag gcacaagtgg ttggcatgca
    21121 ggcgaactgc ccgacaaaag atccattgat gtagcaaaac aacataagct tgatataaca
    21181 agccaacgct caagaaaaat acgcactaca gatatcgaac attttgacct tatactggca
    21241 atggacaagc aaaatcttca ggacttgaag gcttatttca tgaaagatcc tcatctgcat
    21301 tacaaaataa agctcatttc agcattttct aacaaatttc aagacaagga tatacctgac
    21361 ccatattacg ataacgatgg gtttgaacaa gtatttgaaa tgctacagga ttgtattaaa
    21421 ggcctgctgc aaacaacaca caatattcgc tgaactgtta agcgttgatt tgcaaaaaga
    21481 aatacccgtc tttacaaagc ctggaaatat ttgctacaac ggatgaccac ctgctatttt
    21541 tcttcaataa ctgccgacag gcctctatct atcaggccgt ctttgagtgg cctcaactct
    21601 ggttttggag cagtcttaac agtagctttc cctttgaaat gaatgataag agatagttgt
    21661 tcagactgct cgtatgtatg accgcatatt tccatcagac aatgtatgac ccaatcaaaa
    21721 gtattgtaat cgtcgttgta gataatgagt tcggcatttt taccatgact cgtctctagt
    21781 tcagtttcga ccaatacatc ttcctgtgtg tcaaattgcg gaaatgcatt cattttttta
    21841 ccaattttta tgtaaaatta aataaattac tgacacgcgt caagtgcttg ctttatttta
    21901 tcgaagtctg gcaggtctcc ggcactctca agcacttcgg catactggat tattcctttc
    21961 ttatctacta caaaagccga tcgtttggcg acgcctttca tgccaaggat ccaatctttg
    22021 tactgtgcgc cataagcctt gcttactttt ttattaaaat ctgataataa cgggaaattg
    22081 aatccttgct cttccttaaa gcgggcaagt gtaaatactg agtcaactga tacggccaac
    22141 acttctgcat tcaatgaaga atagatgttc atattgtctc ttacagagca caactcggta
    22201 gtacaggtac ccgtaaaagc ctgtgggaaa aacaacagaa ctacatttcg gcctttaaaa
    22261 gatgacaatt ttacttcaga tttatccgat gcaaatagtt tgaataaggg tgccttatcg
    22321 cctggtttta atgacatgat gtttagtttt ttgccttaaa acacaatatt aataataagg
    22381 ttcttaactt cgggaaaatt tttcgggatt ttgaagaggg caaaagagaa atatcacaat
    22441 cttaataatg ttaccaaagc atatctgcag ctacatctgt gtgtttttct ctgggggttt
    22501 actgctattc ttggcaaatt gatttcagta gcggctctgt cactggtatg gtggcgggtg
    22561 gcttttacta ccctgagcct gctgtttgtt aaaagattat tcagcaaaat acgtgaaata
    22621 cctcgccgta aggttggcat ctatatgttt atcggcatcc tggtatgtac gcattgggtt
    22681 ttattttacg catccattaa agcctccaat gcttctgttg gtgtattgtg tatggcatta
    22741 tcgccaattt ttactgcatt tattgaacct ctgtacttca gacgtcggat cctgtggttc
    22801 gagattttgc tgagcctggt agtcatcccg ggtatgtatc tggtgacaca gaatatctct
    22861 gcaagcatga ttacgggttt ggtatatggt atattttccg ctttgtttgc ctcgtggttt
    22921 tctacattga acaaaaaata tatcgaacat tctgaccccg tagcaattac attcctcgaa
    22981 cttggcagtg cctggatttt cctgtctgtc atcatgctgg taagtgctgc agccggtatg
    23041 gatttcagtt tcataccgaa agaaaatgac tggctttacc tgatactgct ttccgtagct
    23101 tgtacggcaa taccttatgt attgtcgctg atgtccctta aatatatttc agtgtttgct
    23161 actaatctta ccattaacct tgagccggtg tatggcatat ttctcgcttg gatactgcta
    23221 caagaagaca aagaagtgga cagcggtttt tatctcggtg ttctgattat acttgcgtct
    23281 gtattcagct
//
LOCUS       DAOQQY010000014        23279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_656_length_23279_cov_5.671245, whole genome shotgun sequence.
ACCESSION   DAOQQY010000014 DAOQQY010000000
VERSION     DAOQQY010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 23279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23279
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_656_length_23279_cov_5.671245"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..491)
                     /locus_tag="PLE23_01630"
     CDS             complement(126..491)
                     /locus_tag="PLE23_01630"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HMX81995.1"
                     /translation="MAKHHHTGQKGEEIAREYLMSHGYQILETNWRFSKAEVDLIAMD
                     GDVMVFVEVKTRSGEHFGFPEQFVSARKEQLMMDAAAVYTSRVGHEWEIRFDIISIIT
                     KGNYSELTHIKDAFFDGWG"
     gene            749..1744
                     /locus_tag="PLE23_01635"
     CDS             749..1744
                     /locus_tag="PLE23_01635"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HMX81996.1"
                     /translation="MVRMDSLVERFVPQLREALEIGKNAVIRPTAEEIRNVFLSGLGG
                     SGIGGNFVQDFVREELKIPFVVGKSYEIPAFVGAGTLAIASSYSGNTEETLSALDKML
                     TTGARVVCVASGGKLIDIAKEKGLDYIKVPDNWPSPRACLGYSIVQQLYILHKLGLIS
                     ERATESINSAAMLLEVEADDIRKKASMIAEMLHGKTPVIYINDRMEAAAIRLRQQINE
                     NAKMLCWHHVIPEMNHNELVGWRTKNDNLAVIILRNKNDHARNQVRIDINKEIIGNYT
                     NTLIEVYSKGSNLVEQMLYFVNLGDWISVYLAELRKVDSIEVKVIDFLKDELARY"
     gene            1816..2412
                     /locus_tag="PLE23_01640"
     CDS             1816..2412
                     /locus_tag="PLE23_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMX81997.1"
                     /translation="MPAHRYFLLNKPYDMVSQFVSSHVVPLLADLPFDFPEGTHAIGR
                     LDRHSEGLLLLTTDKRITKLLFESDTPHARTYMVQVVYAMTERDLESIRSGVKISAKD
                     GTTYTTRPCSASIVEQPGYLWAGHGNIPAHVSSSWVAISLTEGKYHQVRKMVRALGYR
                     CRRLIRTSIEKVSVEGLQPGEVREISREAFFEGLILNT"
     gene            complement(2460..4712)
                     /locus_tag="PLE23_01645"
     CDS             complement(2460..4712)
                     /locus_tag="PLE23_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMX81998.1"
                     /translation="MSDYLDGLNDIQRQAVTDINGPVLVVAGPGSGKTRVLTYRIAYL
                     IQQGVNPRSILALTFTNKAAREMKGRIEKVVGTTANMVWAGTFHSIFARLLRVDAPLI
                     GYPSNFTIYDTDDAKSLINTIVKDLGLDKNTYAAGNILSRISGAKSQIITAEEYVSNA
                     TLRDQDAVVGLPHFAEIYKRYAARMKKAGAMDFDDLLLQLYILLNDFPEVLDKYRRRF
                     AYVLVDEFQDTNTLQYGIIKKLVKYNGSKYNVCVVGDDAQSIYAFRGATIENILNFET
                     DFPMLKTYKLEQNYRSIDAIVQAANEVISHNRKQIKKTIWTDRAEGPGIILLRALNDN
                     EEAKRVVDAILEQKNRNHLRNSEIAILYRTNAQSRIFEEYLRRYNISYRVYGGMSFYQ
                     RKEVKDLLAYFKLSVNPYDDESLKRIINFPRRGIGDATIEGLRQIAVDNDISMWDVLQ
                     SDIPQVKSKKAVTDFTKMIQVFIKKLTELNAYDLAMMIARQSGVLVEYKKENSIDALA
                     RVENLNALLDGVKEFVENDEATPDEDTTSDKSLATYLQTIMLLTDADKESQDSEVVTL
                     MSVHSAKGLEFKSVFVTGVEEKLFPSWMSYDVPDGIEEERRLFYVAITRAETHLTLSY
                     ANSRYRFGKLTYCEPSRFLDELSMIKTQLHAVPQSSAQQLSGVSGNFRPVKMLEKKNL
                     VVDGVDFKVSPPDMVKEGAKVLHLKFGEGIVKKVEGGAGNRVATIYFKDVDDPERRIM
                     LKFAKLQVLS"
     gene            complement(4744..5088)
                     /locus_tag="PLE23_01650"
     CDS             complement(4744..5088)
                     /locus_tag="PLE23_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMX81999.1"
                     /translation="MSMVEEFNEYRSRMNEKILAQDNKTLKRFWALDGGAYAEGALDT
                     KTKELLGLVASMVLRCDDCIKYHLGKCYELNTTTEEVFEVFAIANLVGGSICIPHTRR
                     AVEYWEALAQQN"
     gene            5283..8552
                     /locus_tag="PLE23_01655"
     CDS             5283..8552
                     /locus_tag="PLE23_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMX82000.1"
                     /translation="MKQTLFFMLLSLTYLTTLFSQNTETWYRYPAISPDGKWIVFSSQ
                     GDLYKVSADGGAAVMLTKNDAFDFAPVWSHDSKTIAFASARYGNFDIFTIPAEGGNAV
                     RITMHSNDDIPYDFSADDKSVLFKSVRMDASTNQQFPTGALPELYQVEIKTGKLSQIL
                     TTPAENARYSKDGTMLYYHDAKGYEDPLRKHHTSSVARDVWKYNVKTGEFTKLTSFAG
                     EDRNPVLAADEKEIYYLSESNGSFNVYKMSTTGANPTAVTRLKNHPVRHLSISNAGIL
                     CFSYHGEIYTLKDGGSPKKLNISISVDNLGNQVKNMPVLGDVSEMAVSPNGKEVAFVK
                     RGEVFVTSVKEGTTKRVTNTPEQERSVSFSPDGRSLVYAGERNGSWNIYTSTIARKEE
                     KYFFNSTLLKEEGVVTTDKEEFQPAWSPDGKEIAYLEERTALKVINLDSKKTRLIMPG
                     DKNYSYSDGDQAYTWSPDGKWFLVNFLQDNQWISQCGLVSAEGGKEVFNLTKSGYNNF
                     APKWAMDGKMMTWFSDRDGMKNDASWGGEGDVYGMFFTKEAFDRFKLSKEDFEILKEE
                     EEAAKKDSLKTAEASKKDDKKDDKKDGKKDDKKDKALEPIKIELSGIEDRKVRLTIHS
                     SRLADAVLSKDGEKLYYFAQFEKGYDIWQTELRTKETKIFAKLGANGGGEMYLDKDGK
                     NLFVLADGNMSKIQLDDGKTEPVKIKGDMFLDETAERSYLFEHIWRQVEKKFYVKDLH
                     GVDWNFYKKEYSKKLPAINNNYDYAELLSELLGELNASHTGARYRPGSPNGDRTAALG
                     VFYDNNFNGKGLKITEIMVNGPLSNDKSKIKAGTIIEKVDGTEVNNNEMQYEAFNLKA
                     GKNTLLSLFNPVTGERWDEVVKPISMGEENGLKYKRWVENCRKIVDSLSGGKVGYVHV
                     AGMNDPSYRVVYEEALGRNYSKKALIVDTRFNGGGWLHDDLATFLSGKKYMTLLPRGQ
                     KLGEEPQFKWTKASAVVMNEGNYSDAHMFPYTYKALGIGKLIGMPVAGTGTAVWWETL
                     QNGIVFGIPQVGMVGNDGKFLENTELVPDIKVANEPAAVSKGRDQQLEAAVKELLSEV
                     K"
     gene            8602..9135
                     /locus_tag="PLE23_01660"
     CDS             8602..9135
                     /locus_tag="PLE23_01660"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HMX82001.1"
                     /translation="MDFKLRPWEMADLNSLVKYANNSNISKYLTDRFPFPYTEADGRT
                     FISFASAAKPIHVFAIEINHEAAGGIGIHPQQDIFQKNAELGYWLAEPFWGNGIISKA
                     IVQIMDIAFTEFDIERLFARPFGNNIASQKVLEKNNFRLEARFEKTIFKNGEYQDELV
                     YAYRREMWEKAKVIQQS"
     gene            9473..10516
                     /locus_tag="PLE23_01665"
     CDS             9473..10516
                     /locus_tag="PLE23_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82002.1"
                     /translation="MKPYLTILMFVLCRTLIAQTSESKHLRNITQLTNGGDNAEAYWS
                     FDGKSLAFQSNNKAWGLECDQIFSLNIKKAKKNIKLKPQMISTGKGRTTCSYYMPDNK
                     HILYASTHLGGDKCPADPPKGGKYLWGIFDSYDIFVADIKGNIVKQLTHSPKYDAEAT
                     VSPKGDKIVFTSTRSGDLELWTMDIDGSNQKQITHELGYDGGAFFSPDGTKLVFRSSR
                     PKTPEEIKEYKDYLAQGLVAPTNMEIYTCNVDGSDLRQITHLGKANWAPFYHPGGKKI
                     LFSSNHASKKGYDFQIYMINEDGTGLEQITTESVFNSFPMFSPDGKKLVFSSNRNNGG
                     GHDTNLFIADWVD"
     gene            10516..11757
                     /locus_tag="PLE23_01670"
     CDS             10516..11757
                     /locus_tag="PLE23_01670"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMX82003.1"
                     /translation="MKNLLSNIIVPLCLILGLSGNVIAQNISKSNIRKHIEYLASDEL
                     EGRAPGTKGEQLAIDYIASYFSKLALKPAGTDGYRQQFDYKYHTNPHDTTGTNAVQKK
                     GSNVLGFLDNGAPYTIVVGGHFDHLGKGHHGSALDKGDEIHNGADDNASGTAGVIELA
                     KFFTENGIQEQNNFLFICFSAEEDGLVGSKHFTNTPTVDIGKINFMVNMDMIGRLNPD
                     TRKLLVYGIGTSPHFTGLIDTTDGRFKYVIDSSGVGPTDHTSFYLKDIPVLSFFTGQH
                     SDYHKASDDIEKINFDGEVDILKFIASTILKLDGKGKLPFTPTKQKQQETRSKYKVSL
                     GIMPDYTADVAGLRIDAVVDGKPAQKAGMAAGDIIEAMDGKQIKDVYDYMNQLASYKA
                     GDTAKVRIKRGKESLEINVTF"
     gene            complement(11771..14689)
                     /locus_tag="PLE23_01675"
     CDS             complement(11771..14689)
                     /locus_tag="PLE23_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HMX82004.1"
                     /translation="MENSLKALNEKLTGELHWSAKLRALYATDGSIYRQLPLAVAIPQ
                     TQEDIGLLINLARTHNIGLIPRTAGTSLAGQCVGEGIVVDTSKYFNKIIEINETERWA
                     RVQPGVIRDELNHVLKLAGLMFGPNTSTSNRAMIGGMAGNNSCGSTSVAFGTTRDHVL
                     EMKGFLSDGSAFHAKPLSAEEFYQKTHQDNLEGKIYRHLAEKLSDNQVREEVFREFPK
                     ESINRRNTGYAVDVLLRSEVFTQGGEAFNLSKILCGSEGTLAFTTELKVNLVLVPPPC
                     DALVCAHFDSIHKAMDATVEVMKLKPFACELMDDNILKCTYGTPSFTPLRSFVVGEPA
                     AILIIEQRAETTEKAELLAREIVSVLEKNNLSYANIILHGKEAAKAWALRAAGLGLLA
                     NLEGDEAINCIEDTAVDVEDLPAYIREFEQMMHGYGQDPVYYAHAGDGELHLKPKINL
                     ATKSGIDLFKKITRSSAELVKKFKGSLSGEHGDGRVRAEYIPLMIGQKNYQLIREIKH
                     TWDPQNIFNPGKIVDAFPIDTDLRHQVKHHSTTLKTAFDFGNENGFFNTAEKCTGSGD
                     CRKLPFAGGTMCPSYQATLDEQHSTRGRANALREFISQRPDGKGIEHPELAEVMDLCI
                     QCKGCTTECPSNVDMSRLKSEYQYQVQKLNGPSFRSRIFANNDIINARLRPMAGITNF
                     ILNKSPLSNIIKRMLGIAPQRTFPLVYKKGLRQWFKDYKQEINGEPKGKVIFFCDEFT
                     DLYDAYIGRKAVKLLNRLGVFVIMLPNKPSGRSQVSKGFLNEARKLADFNVQLLSEDR
                     LKDIPITGIEPGAMLIFRDEYPDLVSTQNKKKATELAKRVFLIEEYLYDLLCNGNVDT
                     SGWKAFSSKIMYHAHCHQKSLSDAGKAAWLLSAAPGAQVEIIPSGCCGMAGSFGYEKE
                     HYQISMKIGSLVLFPAINKQTDDVVIAASGDSCRHQIKDGTGRQAVHPVEILYEYAGG
                     "
     gene            complement(14756..15112)
                     /locus_tag="PLE23_01680"
     CDS             complement(14756..15112)
                     /locus_tag="PLE23_01680"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HMX82005.1"
                     /translation="MELKGRIIHILPIQTGEGKNGPWQKQDYVLETLDTYPKKVCFNL
                     WGDKVNQFAIKQDEMLTVHFDVESREFNGKWYTDVKAWRIDRNIDSADAPGQAQAADM
                     PPPADFGSGEDDDLPF"
     gene            complement(15132..16676)
                     /gene="lysS"
                     /locus_tag="PLE23_01685"
     CDS             complement(15132..16676)
                     /gene="lysS"
                     /locus_tag="PLE23_01685"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMX82006.1"
                     /translation="MGHNELTEQEILRRESLDKIRELGIDPYPAALYPVTHLSSEIKE
                     NYKEEILEDGTKNRLNFQDVCLAGRLMNMREAGKAMFMNIQDADGRIQLYLRREELSE
                     NDDYTFFDQLIKKLLDIGDYIGVKGFAFKTKTGETTIHVTSFTLLSKSLKPLPVVKTD
                     AEGNVFDAVSDPEFRYRQRYVDLTVNQGVKEVFLKRTKMVNAIREYLNEHGGIEVDTP
                     VLQNIPGGAAARPFITHHNALDVPFYLRIANELYLKRLIVGGFDFVYEFSRNFRNEGM
                     DRTHNPEFTVLEFYVAYKDYFWMMDFTEKLLEKVALASNGTTDLPVGNNIISFKAPYK
                     RIALYDAIKEYTGFDVNGMDETALRDVCKKLNIPTDESMGVGKLIDEIFSEKCEKNFI
                     QPTFIIDYPEEMSPLTKKHRSKPGLVERFELMVNGKEVANAYSELNDPIDQRMRFEEQ
                     AQLMERGDDEAMFIDHDFLRALEYGMPPTSGIGIGIDRLAMLLTNQQSIQEVLFFPQM
                     KPKQEN"
     gene            16835..21934
                     /locus_tag="PLE23_01690"
     CDS             16835..21934
                     /locus_tag="PLE23_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX82007.1"
                     /translation="MRMRFLYHKVCIFTILNIFFITQLTSQCAEITASSINGPCTNGS
                     TPCDVCPGAVLTLKAEGANLINNGCVDWYYSDDQNFDPYQNEGTFIACGKITTTFPPF
                     CAVCPTILAMMIDACGPEGPNEFMLLSSGSGFNLNNLQIDFAVQNNQMGNNNNDINIG
                     ASPCGWKTPNASLIAMILASANCTAANVIPVGPGESVPPNGLVVVFTSGDEFSNYDFS
                     ALCATGQKLYVVQNACNRTVGAFSNGSSSGSRTTTISIAGCSCTNSLTYTCEDLAPAQ
                     DGDYVLADGTLGNDGCTAPDVIAPPVMSTSTIADVTLATTEAMCNMGPYYIKGILNPP
                     ADPLCPDVYTGTFAFNVVCPEAEAMASSPTCWGGSVNLEANGGDTYKWSGPGGFNSIQ
                     QNPVITGLNNLKSGTYTVTVTNAAGCTDVASVQVTVNPDITVSITPANPAFCEGESVT
                     LTGNATGGSGNFDFEWTEPGGGMVNGQTISASVPGTYTLVVTDGEGCTKSASINVIQN
                     PSPDVDITPMPAKVCEGQTKTITANATGGSPAYNYSWSNGMSSAAITVGIGTYNVTVT
                     DAKGCKGFANIEVENYDPIDITFIQNPDVLCNGASATLTANATGGTGTFTSYQWTTPG
                     GPASGSSITVSQAGNYTVTITDNAACTGTAVHTIIINPILNVSINPNPASFCAGGSVD
                     LTANATGGSGSGYLYNWSTPSGNKTGQVVTATLAGSYSVTVTDGEGCTGTAVVNVTEN
                     ANLSVNILPATPSFCPGSFVNLTATPSGGTTPYSFSWTTPGGTKSGQDIQANIAGNYT
                     VTVSDSKGCTGTATVNVTESPGFPVNITPPNPSICPGGKIDLTAVPGGGNGTFTYAWT
                     TPGGNKTGATVTATVVGTYNVTVTDSGGCSGTAQTTVVENPNLAVSILPAAPTFCPGE
                     TVDLTVNAPGNIASYSWTTPGGSKSGSPLTVKTAGNYTVTVTDTGGCSGTATVNVSEN
                     PAKTVTISPANPGFCSGSSVELTVNATGNNLVYNWTTPSGTANGNPLIANVAGIYTVT
                     VTEQGACSGTASVQVDEVSGLTVDINPKPASFCNNGSVLLTASSSNGIAPFTYSWSSP
                     AGNGTGMTFNANIAGNYTVTITDSKGCSGSFTIPVVQSNSLIVTFNPVLPGFCPGKTV
                     DISTNTIGGQAPFSYQWSSPLGTSANPVITTGTPGNYKVTVTDSNGCSGLGEVVVDQF
                     SEPILTLPSAPGFCPGNNVALDVTVNDNTPPYIYTWTTPSGNFNTKTITANIAGNYTI
                     SVTNANNCTASQSVNVTEYPAPIAEIIPDPVVFCKNSSATVTANPSGGAAPYTFLWSG
                     PATNTTATNIQINTAGVYTLLVTDAHSCTADYTFPANEQPGLLVSLSTDPQKLCGVAQ
                     FNVNANVTGGNPPYTYSWNTPGGTQTGSTISGNESGTYSVTVTDNEACSGVADLLINN
                     KPLNALVTPVDPTCKSPAGGSIALVFDTGIALPANINLNNNSAGQITQTTHTINGLAP
                     GNYQLLVTDAKGCEFQTTVTLAPLPVLHLDLGQDVTINLGDTYTIHPITDFAINNISW
                     TNEQSLNCPDPCINPVAGPVNTTTYKASAYDENGCLAEDELTIYVHIKTTVYVPNSFS
                     PDADGINDKWVIFADNTVKLIKTLHIYDRWGESIMQQENFPPNDPDYGWNGTFRRKPL
                     DPAVFVYYFVVEYENGTTKVFKGDINIISKK"
     gene            21931..22728
                     /locus_tag="PLE23_01695"
     CDS             21931..22728
                     /locus_tag="PLE23_01695"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMX82008.1"
                     /translation="MTEDYIEINRKLWNDKVGIHVDSEFYDQQSFLAGKSSLKRPELD
                     LLGDITGKKILHLQCHFGQDTMSLSRMGGHATGIDFSEKAIEIANETTKSLSLGTSFV
                     CCDVYHTPAFIDQQFDIVFTSYGTIGWLPDLQKWASVIYKMLKPGGKLIFVEFHPFVF
                     TLDEEFDFVKYFYFNKEEIVETEEGTYAEKNAQISNKSISWNHPISEVLQNLIDAGLT
                     ISRFEEYDYSPYACFSKLVKQGEDEYRVEGLEGKLPMVYAIVANKAE"
     gene            complement(22748..>23279)
                     /gene="upp"
                     /locus_tag="PLE23_01700"
     CDS             complement(22748..>23279)
                     /gene="upp"
                     /locus_tag="PLE23_01700"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HMX82009.1"
                     /translation="IFAYEISKTLDFYEQEVTTPLGTAMVRKTERKVVLATILRAGLP
                     MHQGMLNYFDYAGNAFISAYRKHHKDGSFEISLQYVTCPDLNDAVLIIADPMLATGQS
                     IARTLQELTRYGHPKSVHIVSAIAAREGVDHVRRHLPNATLWLGDVDEELTAKSYIVP
                     GLGDAGDLSFGDKIQN"
BASE COUNT         6680 a         5104 c         5207 g         6288 t
ORIGIN      
        1 agaagccggc acaaagtaag gcggatagcc cgacccgaca ggggcacacc caaataataa
       61 tgaatgatgt tatcgctatt tttcatccag tgtacgatat actaaacact aagctacaga
      121 caggctcagc cccaaccatc gaaaaaggcg tctttgatgt gtgtaagttc ggagtagttg
      181 cctttggtga tgatagaaat gatgtcgaag cggatctccc actcatggcc gacccggctg
      241 gtatatacgg cggcggcatc catcatgagc tgctctttgc gagctgaaac gaattgctcg
      301 ggaaagccga aatgctcacc cgaacgggtc ttgacctcga cgaataccat gacgtcgcca
      361 tccatagcta tgaggtcaac ctcggctttg ctgaagcgcc agttggtctc gaggatttgg
      421 tatccgtgcg acattaggta ttcgcgagct atctcttcgc ctttttgtcc ggtgtgatgg
      481 tgttttgcca tggttcaaag ctaatttaaa gttttgaacc aaacaagtag gtgcatttgt
      541 tcgttttaat atatcagata agacattagg ctctttacgg caaataatga ttgctttaaa
      601 tccgttgtac aagctcaaaa aaaccatagt ttactatgtt tttatccctt ttatatggat
      661 tttttgaatt aattattttc atttaaattc ctaatgcgtt atccgggata aattatcttt
      721 gtcgctttat ataaaaagaa taattcttat ggtaaggatg gattcgctgg tggagcggtt
      781 tgtaccacaa ctgagggagg cgctcgaaat cggtaaaaat gctgttatcc gccctacggc
      841 tgaggagatt cggaatgtgt ttctctcagg tcttggtggc tctggtatag ggggcaattt
      901 tgtacaggac tttgtaaggg aagagttgaa aattccgttc gtagtaggca agtcgtacga
      961 aatacctgct tttgtaggtg ccggcacatt ggctattgca tcatcgtact ccggcaatac
     1021 ggaggagacg ctatcggcac tggacaaaat gcttacaaca ggtgccaggg tagtgtgtgt
     1081 ggcctcgggt ggtaagttga tagatatagc gaaagaaaag gggcttgact atataaaagt
     1141 gccggacaac tggccatcgc caagggcgtg cctgggttat tcgatcgtac agcagttgta
     1201 tatactacat aagcttggcc tgataagcga acgtgcaaca gaatcaataa actcggcggc
     1261 tatgttgctc gaagtggagg cagatgacat ccgcaagaag gcttcgatga tagccgaaat
     1321 gctgcatggc aagacgccgg taatttacat caacgaccgg atggaggcag ccgcaataag
     1381 gctgaggcaa cagataaacg aaaatgccaa gatgctctgc tggcaccacg tgataccgga
     1441 aatgaatcac aacgagctgg taggctggcg cacaaagaat gacaacctgg cggtgatcat
     1501 actccgaaat aagaacgacc atgcacgcaa ccaggtgcgt atagacatca acaaggaaat
     1561 aataggcaat tataccaata cgctgataga ggtgtattcg aaaggcagta atctggtaga
     1621 gcagatgttg tacttcgtca acctcggcga ctggatatcg gtatatctgg ctgaactgcg
     1681 caaggtggat agtatagaag tcaaggtcat cgacttcctg aaagatgagc tggcaaggta
     1741 ttgaattatt tgtttttttt cttatgataa aataatcttc tacatccgcc aacatcagac
     1801 atacaaaacg ctcaggtgcc agcacacaga tatttcttac tgaacaaacc ttatgatatg
     1861 gtttcgcagt ttgtgagcag ccatgttgtg ccattgttgg ccgatttgcc ttttgatttt
     1921 ccggaaggaa cgcatgccat aggccggctc gaccgccact cggaaggatt gctgctgctg
     1981 accaccgaca agcgcattac caagctcctg ttcgaatcgg acacaccgca cgcgcgcact
     2041 tatatggtgc aggtggtata tgccatgacc gagcgggatt tagaaagtat caggagcggt
     2101 gtaaagatat cggccaaaga tggtacgaca tacacaacaa ggccatgcag tgcatcaata
     2161 gttgaacaac ccggatatct ttgggctggt catgggaata ttccggcaca tgtatcttcg
     2221 agttgggtag ccataagcct gactgaaggt aaataccatc aggtgcggaa aatggtacgg
     2281 gcacttggtt atcgctgtcg cagactcata cgcacttcga tagaaaaggt aagtgtagaa
     2341 ggactgcaac ccggagaagt acgagagata agccgggaag cattttttga aggattgatc
     2401 ctgaacacct aaggacaatg tgcacaagat aaggtcggat atttgctgtc aaaaccggat
     2461 cagctcaata cctgtagttt ggcgaacttg agcataattc ggcgttcggg gtcgtccaca
     2521 tccttaaaat agatagtagc tactcggttg cctgcgccac cttccacttt tttgacgatg
     2581 ccttcaccga acttcaggtg tagcactttg gcaccttctt tcaccatatc gggcgggctc
     2641 accttgaagt caacaccatc cactacgagg tttttctttt ctagcatttt caccgggcgg
     2701 aagttaccgg acacgcctga tagctgttgg gcagagcttt gcggaacagc atgtagttgc
     2761 gttttgatca tagagagctc atcgaggaag cggcttggct cgcagtaggt gagtttgccg
     2821 aagcggtatc ttgagttggc ataagacagt gtaaggtgag tctcagcacg ggtaatggcc
     2881 acataaaaca ggcggcgttc ttcttcgata ccatccggca catcatacga catccagctt
     2941 gggaagagtt tttcctcgac acctgtaaca aacaccgact taaactcaag gcctttggca
     3001 gagtgcacag acatgagtgt cacgacctcc gaatcctgac tttctttatc ggcatccgtg
     3061 agcagcatta tggtctgtag gtaggtagcc aaagatttgt cgcttgtagt atcttcatca
     3121 ggtgtggctt catcattttc tacgaattcc ttaacgccgt cgagcagggc attgaggttt
     3181 tcgacacgag ccagtgcgtc tatggaattt tcctttttgt attcaaccag cacaccgctt
     3241 tgacgggcaa tcatcatagc gaggtcgtag gcattgagct ctgtaagttt tttgatgaat
     3301 acctgaatca tttttgtaaa atcagttact gctttcttgc ttttcacttg cgggatatca
     3361 gactggagca cgtcccacat tgatatatcg ttgtcaacag ctatttgtcg cagaccttct
     3421 atggtggcat cgccaatgcc tctacgaggg aagttgataa tacgtttgag cgattcgtca
     3481 tcgtaggggt tgacactcag tttgaagtaa gcgagcaggt ctttcacttc cttgcgttgg
     3541 tagaagctca tgccgccata tacgcggtag ctgatgttgt accggcgcag gtactcttca
     3601 aaaattcttg actgtgcatt tgtacggtag aggatggcaa tctcgctgtt gcgaaggtgg
     3661 ttgcggtttt tttgttcgag gattgcatcg accacacgtt ttgcttcttc attgtcgtta
     3721 agcgcccgga gcagaatgat acccggccct tcagcgcggt cggtccagat tgtttttttg
     3781 atttgtttgc gattatgact gataacctca ttggctgcct gaacgatagc gtcgatagag
     3841 cggtagtttt gctcgagttt ataggttttc agcatcggga agtcggtctc aaaattgagg
     3901 atattctcga tggtagcccc acggaatgcg tagatacttt gggcgtcatc gcctaccaca
     3961 cagacgttat atttacttcc gttgtacttg accagttttt tgatgatgcc gtactgaagt
     4021 gtattggtat cctgaaactc atcgacgaga acataggcga accggcggcg gtatttgtcg
     4081 agtacttctg gaaaatcatt gagcaatatg tagagttgga gcagcagatc gtcaaagtcc
     4141 atggcaccgg ccttcttcat tctggcggca taacgtttgt agatttcggc gaaatgtggt
     4201 aaacctacaa cggcatcctg gtcgcgcagc gttgcatttg atacatattc ttcggctgta
     4261 attatctggc ttttggcacc tgagatgcga ctgagtatat taccggcagc gtaggtattt
     4321 ttgtcgaggc cgaggtcttt aactattgtg ttgatcaggc ttttggcatc gtcagtgtca
     4381 tagatggtaa aattggacgg gtagccgatc aaaggtgcat cgacacgaag caatcgggcg
     4441 aatatcgagt gaaaggtacc tgcccaaacc atattggcgg tagtgcctac taccttctca
     4501 attctgcctt tcatttcgcg ggcagcttta ttggtaaagg taagggccag tatgctgcgc
     4561 ggattgacgc cttgctgtat gaggtaggct atacggtatg tgagcacacg ggtttttccg
     4621 gagccagggc ctgctaccac caatacagga ccgttgatgt ctgttacagc ctggcgttgt
     4681 atatcgttaa gtccgtcaag ataatctgac attgaaagat tgtttgttgt gttggataaa
     4741 aaatcagttt tgttgtgcca gtgcctccca atactcgact gctctacggg tatgtggtat
     4801 gcagatagag cctccgacca ggttggctat ggcaaacact tcaaatactt cctcagtagt
     4861 ggtattcagt tcatagcatt tgcccagatg atacttgata caatcatcgc agcgcaagac
     4921 catagaagca acgaggccga gtagttcctt ggttttggta tcgagtgcac cctcggcata
     4981 ggcaccaccg tcgagtgccc aaaagcgctt cagcgtttta ttgtcctgag caaggatttt
     5041 ctcgttcatg cgggagcggt attcgttgaa ttcctctacc attgacatac tgtgtagttt
     5101 tgttgaaggc ctcaaaggta cgaataaggt gggtgtataa aaaaatttaa tagttgtcct
     5161 tttataaaaa tcacaaaggc tttgtctttt gggtatgtta aataatgtta attatgtaaa
     5221 tttttgatcc gtgaaatatt caaatacata tatttgccca cttcaactca aaaccaatga
     5281 atatgaaaca aacactgttc tttatgttgt tatcgctaac atatttaaca acattatttt
     5341 ctcaaaatac tgaaacatgg tacaggtatc cggccatttc gcctgatggt aaatggattg
     5401 tattctcaag ccagggcgac ctatacaaag tgagtgctga tggaggtgct gccgttatgc
     5461 ttacaaaaaa tgatgcattc gactttgcac ctgtatggtc gcatgacagt aaaaccatag
     5521 cctttgcatc agccagatac ggcaatttcg atatattcac cattcctgca gaaggtggca
     5581 atgctgtaag aataacgatg cattcaaatg atgacatccc ttatgatttt tcagctgatg
     5641 acaaatctgt tttattcaag tcggtcagga tggatgcttc aactaaccag caatttccga
     5701 caggcgcact acccgagctg taccaggtag aaataaagac cggaaagcta agccaaatat
     5761 taactacacc agccgaaaac gctcgttaca gcaaggatgg gactatgctg tattatcatg
     5821 atgcaaaagg ttatgaagac cctttgcgca agcaccacac ttcatctgtt gctcgtgatg
     5881 tatggaaata caacgtaaaa acaggcgaat tcaccaagct gaccagtttt gccggagaag
     5941 accgcaatcc ggtattggca gcggatgaaa aagagattta ttatctgagt gaatcgaatg
     6001 gctcgttcaa tgtgtataag atgagcacga ccggggctaa cccgacagca gtcacccgtc
     6061 tgaaaaacca ccctgtgcgc cacttgagca ttagcaatgc aggcattttg tgtttttcat
     6121 accatggtga aatatacact ttgaaggatg gtggctcacc aaagaagctc aacatcagca
     6181 tctccgttga taacctgggc aatcaggtaa agaatatgcc tgtgctcggc gacgtgtcag
     6241 agatggctgt ttcgcccaac ggcaaggaag tggcatttgt taagagagga gaagtgttcg
     6301 ttacttcagt aaaggaagga accaccaaac gcgtaaccaa cacacccgag caggagcgca
     6361 gtgtcagttt tagtcccgac ggccgctcgc tggtatatgc cggagagcgc aatggctctt
     6421 ggaacatcta cacaagcacc attgccagaa aagaggaaaa atacttcttc aactctactt
     6481 tactcaagga agaaggtgta gttaccaccg acaaagagga atttcaaccg gcatggtcac
     6541 ctgacggaaa ggaaatagcc tatctcgaag aacgcacagc tttgaaggtc atcaatcttg
     6601 attcaaagaa aacaaggcta ataatgccgg gtgacaaaaa ttattcgtac tcagatggcg
     6661 atcaggcata tacatggtct cctgacggaa aatggttttt ggtcaatttc ctccaggaca
     6721 accagtggat atcgcagtgt gggctggtaa gcgcagaagg tggcaaggag gtattcaacc
     6781 tcaccaaaag tggttacaac aactttgcac caaaatgggc aatggacggc aagatgatga
     6841 cctggttcag tgaccgagat ggtatgaaaa atgatgcaag ctggggtggc gaaggtgatg
     6901 tttacggaat gttttttacc aaagaagcct ttgacagatt caaactcagc aaagaagatt
     6961 tcgaaatatt gaaagaagaa gaggaggctg caaaaaaaga cagcttaaaa accgctgaag
     7021 ccagcaagaa agatgataaa aaagatgata agaaagacgg caaaaaggat gacaagaaag
     7081 acaaagcatt agaaccaatc aagatagaac taagtgggat agaagacagg aaagtgagac
     7141 tgaccataca ctcatcgcgt ctggcggatg ctgtattgtc gaaggatggt gaaaaactct
     7201 attattttgc ccaattcgaa aaaggttacg atatatggca aaccgaactc cgaaccaagg
     7261 aaacaaaaat atttgctaaa cttggtgcca atggcggagg cgaaatgtat ctcgacaagg
     7321 acggtaagaa tctgtttgtg cttgccgacg gaaatatgtc aaaaatacag cttgacgacg
     7381 gcaaaacaga gcctgttaaa atcaaaggtg acatgttctt ggatgaaaca gcagaacggt
     7441 cttacctgtt cgagcatatc tggcgtcagg tggagaagaa attttatgta aaagacctgc
     7501 atggtgttga ctggaatttc tacaaaaaag aatacagtaa aaaactacct gccatcaata
     7561 acaattatga ttatgcagaa ctactgagcg aactacttgg cgaactcaac gcttcgcata
     7621 caggtgcgcg ataccgaccc ggctcaccaa acggagacag aactgctgct cttggtgttt
     7681 tttatgacaa taattttaac ggcaaaggtt tgaaaataac cgaaataatg gtcaacggtc
     7741 cgctatcaaa tgacaagtcg aagatcaaag ccggtaccat catcgagaaa gtggatggca
     7801 cagaagtgaa caataacgag atgcagtacg aagcatttaa cctgaaggca ggcaagaaca
     7861 cacttctatc attattcaac cctgttacag gcgaaagatg ggacgaagta gttaaaccta
     7921 taagcatggg tgaggagaac gggctcaaat acaaaagatg ggtagagaac tgtagaaaga
     7981 tagtggacag cttatcgggc ggaaaagttg gttatgtaca tgttgccggt atgaatgacc
     8041 caagctatcg tgtggtttat gaagaagcat taggtcgcaa ttattcgaag aaggcactga
     8101 tagttgacac aagattcaac ggaggcggtt ggctgcacga cgaccttgct accttcctga
     8161 gcggcaagaa gtatatgacc ttgttgccaa gagggcaaaa attgggcgag gaaccacagt
     8221 ttaaatggac aaaagcttca gctgtggtca tgaatgaagg caattattcg gatgcacaca
     8281 tgtttcctta cacatacaag gcacttggca ttggcaagct catcggtatg ccggtggcag
     8341 gaacaggaac tgccgtctgg tgggagacac tccagaacgg tatcgttttt ggcattccgc
     8401 aagtaggtat ggtaggcaat gatggcaaat ttctcgaaaa taccgaactc gtgccggaca
     8461 taaaggtagc taacgaaccg gctgctgtaa gcaagggcag agaccaacaa ctcgaggctg
     8521 cagtgaagga acttttgtca gaagtgaaat aaaacgagca aaaccggaat acaaaattcg
     8581 ttacagaaat gattcggtag tatggatttc aaattaaggc catgggaaat ggcggatctg
     8641 aatagtctgg tcaaatatgc caacaacagc aatatatcga agtacctgac tgacagattc
     8701 ccgtttccct ataccgaagc agatggccgg acattcatat cctttgcgtc agctgcaaag
     8761 ccgatacacg tatttgccat tgaaattaac catgaagcgg caggaggtat cggcatacat
     8821 cctcaacaag atatttttca gaaaaatgcc gaactcggct actggttggc tgagccattc
     8881 tggggaaatg gtataataag taaagcaatc gtgcagatca tggatattgc ctttacagag
     8941 tttgacatag aaagattatt tgcaaggcca tttggtaata atatagcatc acaaaaagta
     9001 cttgaaaaaa acaatttcag actggaagcc aggtttgaaa aaacgatatt caaaaacggt
     9061 gaataccagg acgaactcgt gtatgcctac agaagagaaa tgtgggaaaa agccaaggtg
     9121 atacaacaaa gctaaaaaat aagattttta ctgttgaaaa attatctttg ccgcccaaga
     9181 caaatcttat cagatataaa aaactttttc acgcttgaaa gaagcgaata tttaggaaga
     9241 aaattttcca atacggaggg aaccggataa ttggatgaaa gcaattaaca ttttgtactc
     9301 gtgtagccaa tttccactca aaaaaatgaa gaagacatat tagctggggc ataatttgta
     9361 caaatgccaa gcagaaatgt gtcataccac ttccctatcg caagtttgac aaaaatgtga
     9421 cgagttttat ttatctgtga aatatttttg cggcaatcaa actaagtaca acgtgaaacc
     9481 ttatcttact atccttatgt tcgttttgtg ccggacactc attgcacaaa catctgaaag
     9541 taaacacctg cgcaacatca cacaactcac caacggaggt gacaatgccg aagcctactg
     9601 gagctttgac ggaaaatctc tcgctttcca aagcaacaac aaagcctggg gactggaatg
     9661 cgatcagata tttagcctca acatcaaaaa agctaaaaag aacatcaagc tcaagccaca
     9721 gatgattagt accggtaaag gccgtactac ctgctcgtat tacatgcccg acaataaaca
     9781 catcttgtat gcctctacgc atctcggtgg cgacaaatgc cctgccgacc cacctaaagg
     9841 cgggaaatat ctctggggaa tatttgacag ctacgacatt tttgtagccg acatcaaggg
     9901 gaatatcgtc aaacaactta cccattcacc caaatatgat gccgaagcaa cagtctctcc
     9961 aaaaggtgat aaaatcgtgt tcaccagcac acgcagcggt gatctcgaac tttggacaat
    10021 ggacatcgac ggttcaaacc aaaaacaaat aacacacgaa ctcggttatg atggaggagc
    10081 tttcttctca ccggatggta ccaagttagt tttcaggtct tctaggccaa aaactccgga
    10141 agaaatcaag gaatacaaag attaccttgc acaaggttta gtagcaccga ccaatatgga
    10201 aatttatact tgtaatgtgg acggctctga tttgagacaa atcactcatt tgggtaaagc
    10261 taactgggcg cccttttatc acccgggtgg caagaagata ttgttttcga gtaaccatgc
    10321 ctccaaaaaa ggttatgact tccagatcta tatgatcaac gaagatggta caggtcttga
    10381 acaaataacc acagagagcg tcttcaattc gttcccgatg ttttcaccgg acggcaaaaa
    10441 actcgtattc tcatctaacc gtaataacgg gggcggacat gacaccaatt tattcatcgc
    10501 agactgggtt gactaatgaa aaacttacta tctaacatca tcgtgccact gtgtttaatc
    10561 ctagggctaa gtggaaatgt catagcgcag aatatcagca aatcgaatat ccgcaagcac
    10621 attgagtacc ttgcctcaga cgaactcgaa gggcgcgcac cgggcactaa aggcgaacag
    10681 ctggctattg actatattgc atcttatttc tccaaactgg cactaaaacc agccggcacc
    10741 gacggctacc gacaacaatt tgactacaaa taccatacta atccacatga cacgaccggt
    10801 accaatgctg tacagaaaaa aggcagcaat gtgttaggtt ttcttgataa cggagcgcct
    10861 tacaccatcg tcgtaggcgg ccatttcgac cacctcggca agggacacca tggttcggct
    10921 ttggataaag gcgacgaaat acacaatggt gcagacgaca atgcatcagg tactgccggt
    10981 gttatcgaac tcgcaaagtt ttttaccgaa aacggcatac aggagcaaaa caattttttg
    11041 ttcatctgtt tttcagctga ggaagacggt cttgtgggct ccaaacattt caccaatact
    11101 cctacggtcg atattggcaa aattaatttt atggtcaata tggatatgat cggaagactc
    11161 aatccggata cccggaagtt acttgtatat ggaataggta cgagtccgca ttttacggga
    11221 ttgattgaca ctacagacgg tcggtttaaa tacgtaatcg attcatcagg tgtagggcct
    11281 accgaccata cttcgtttta cttgaaagac ataccggtac tcagtttctt caccgggcag
    11341 cacagcgatt atcacaaggc aagcgatgat atagaaaaaa ttaattttga cggcgaggtt
    11401 gatattttaa aattcattgc atccactatc ctaaaacttg acggtaaagg caaactcccg
    11461 tttacgccta ccaaacaaaa acagcaagaa accagatcga agtacaaggt ttctcttggt
    11521 ataatgcccg actataccgc tgatgttgcc gggcttcgga tagatgcagt cgtagatggc
    11581 aaaccagccc aaaaagcagg catggcagca ggtgacatca tcgaagcaat ggacggcaaa
    11641 caaataaagg atgtctatga ctatatgaac caactggctt catacaaagc cggtgatact
    11701 gcaaaagtta gaatcaaacg gggtaaggaa agtcttgaaa taaacgtcac attttaacta
    11761 taaagcaaca ttacccacct gcatactcat aaaggatttc gaccggatgt actgcttgtc
    11821 gtccggttcc atcctttatc tggtggcggc agctatcacc tgatgctgca attactacat
    11881 catctgtctg tttgttaatg gcaggaaata atacaaggct gcctatcttc atgcttatct
    11941 ggtaatgttc tttttcgtaa ccaaaactgc cggccatacc acaacaaccg ctcggtataa
    12001 tctctacttg cgccccgggt gctgcactca gcagccatgc tgccttaccg gcatcactca
    12061 atgatttttg atgacaatgt gcgtgataca ttatttttga agagaatgcc ttccagcccg
    12121 aagtatcaac attcccattg cacaacaaat catacagata ttcttcaatg agaaaaaccc
    12181 ttttcgcaag ttcggtggct tttttcttgt tttgtgtact taccaagtca ggatattcgt
    12241 cgcgaaatat cagcatggca ccgggttcta tacctgttat gggaatgtcc ttcaatctgt
    12301 cctctgacaa aagctgcaca ttgaagtcag ccagctttct tgcctcgttg aggaaaccct
    12361 ttgacacttg acttcttccg ctaggtttgt tcggcaacat gataacaaat actccgaggc
    12421 gattaagcaa ttttacagcc ttacggccaa tgtaagcatc atacaaatcg gtaaattcat
    12481 cgcagaaaaa aatcactttc ccttttggct caccatttat ttcttgtttg taatctttaa
    12541 accactgacg caaccccttt ttatatacta gaggaaaagt tctttgtggt gcaataccca
    12601 acattcgctt aatgatattt gaaagtggac ttttattcag gataaaattg gtaatacccg
    12661 ccattggtct cagtcgggcg tttattatat cattattggc gaatattctg cttctgaatg
    12721 atgggccatt aagtttttgc acctgatact gatattccga tttcaggcgc gacatatcta
    12781 cattcgacgg gcattctgtt gtacagcctt tgcattgtat gcacagatcc attacttcgg
    12841 caagttcagg atgttctata ccttttccat cggggcgttg cgatataaat tcccgcaggg
    12901 cattggccct ccctctggtc gaatgctgtt catcgagtgt tgcctgataa ctcgggcaca
    12961 tggttcctcc ggcaaaaggc agcttacggc agtcaccgga cccggtacat ttttcagctg
    13021 tattgaaaaa accattttca ttaccaaaat caaaagctgt tttgagtgta gtagaatggt
    13081 gttttacctg atgcctcaga tctgtatcaa tagggaatgc atctacgatt ttccccgggt
    13141 taaaaatatt ttgcggatcc caagtgtgct ttatttccct tataagctga taatttttct
    13201 gaccaatcat cagtgggata tattctgcac gcactcgtcc gtcgccatgt tcaccactga
    13261 gtgatccttt gaatttcttg acaagttcag cagacgaacg cgttattttc ttaaacaaat
    13321 cgattccgga ttttgtagca aggtttattt tgggtttcag atgcaactcg ccatcacctg
    13381 catgtgcata gtacaccgga tcttgtccgt aaccatgcat catttgttca aattctcgaa
    13441 tatatgccgg cagatcttca acatcaacgg ctgtgtcttc tatacagtta atggcttcgt
    13501 caccttcgag gttggcaagt agcccaagcc cggctgctct cagagcccat gctttggctg
    13561 cttccttgcc atgcagtatg atgttggcat agctcagatt atttttttcg agtacactta
    13621 ctatttctct ggcaagtagt tcggcctttt cggttgtttc tgcccgctgt tctatgatta
    13681 gtatagcagc cggctcacct acaacaaatg agcggagtgg tgtaaacgaa ggtgtaccat
    13741 aggtacattt cagaatgttg tcatccataa gttcacaggc gaacggcttc aacttcatta
    13801 cctcgactgt ggcatccatt gctttgtgta tggaatcgaa atgtgcacaa accagcgcat
    13861 cgcaaggcgg tggcaccagt acaaggttta ctttaagttc ggtagtaaag gcaagcgttc
    13921 cttccgatcc gcagagaatt tttgacagat taaaagcttc ccctccttgt gtaaaaacct
    13981 cacttctgag caagacatct actgcatatc ctgtatttcg cctattgatg gattccttcg
    14041 gaaattctct gaaaacttct tcgcgaactt gattatcgga taatttttct gcaagatgcc
    14101 ggtatatttt tccttcaaga ttgtcttgat gtgttttttg gtaaaattct tcagcggaca
    14161 acggctttgc gtggaatgct gatccatcgc tcaggaaacc tttcatttcc aggacgtggt
    14221 cacgtgtcgt accgaatgca actgaagtag agccacaact gttattgccg gccatgccac
    14281 ctatcattgc acggttggaa gttgaagtat taggaccaaa catcagccct gccagtttta
    14341 gtacatggtt gagctcatcc cgtatgacgc ccggttgtac tcttgcccag cgctcagttt
    14401 cattaatttc aatgattttg ttgaaatatt ttgaagtgtc caccacgatg ccttcaccaa
    14461 cacactgtcc tgccagcgat gtgccggcag ttcttggaat aaggccaata ttgtgcgtcc
    14521 gggctaaatt gatgagtagc ccgatatctt cttgtgtctg tggtatagct acggcaagcg
    14581 gcagttgcct gtagatagag ccgtctgtag catacagcgc cctgagtttt gcagaccagt
    14641 gcaattctcc cgtgagtttt tcattcagtg ctttcagcga attttccatt gcgcttgaaa
    14701 gttaagaaaa aagcttgagc ttatgttgtt tgggcaattt ttcttcaaaa aattttcaga
    14761 aaggaaggtc gtcatcttct ccagagccaa aatcagccgg tggtggcata tctgcggcct
    14821 gtgcctgacc aggtgcatca gcagagtcaa tgtttctgtc tatgcgccat gcttttacat
    14881 cggtgtacca tttgccgtta aactctctgc tttctacatc gaaatgtact gtcagcatct
    14941 cgtcctgctt gatagcaaac tgatttactt tatcacccca taggttgaag caaactttct
    15001 taggataggt atcaagggtt tcgagtacat aatcctgttt ttgccaggga ccgttcttcc
    15061 cttctccagt ctgtattggt aatatgtgaa tgattctacc tttaagttcc atgattatta
    15121 tattatttat attaattttc ttgttttggt ttcatttgcg gaaagaataa cacttcctgt
    15181 atagattgct ggttagtcaa aagcatggcg agacggtcga taccgatacc gataccggag
    15241 gttggcggca ttccgtactc aagagcccgt aggaaatcat gatcgataaa catggcttca
    15301 tcatcaccgc gttccatcaa ctgtgcctgt tcttcgaacc tcattctctg gtctatcgga
    15361 tcgttcaact cgctataggc attggctact tcttttccat tcaccatcag ttcgaaacgc
    15421 tccaccaagc ctggtttgct tcggtgcttt ttagtaagtg ggctcatttc ttccggataa
    15481 tcgatgataa atgtaggctg aatgaagttc ttttcacatt tttcgctgaa tatttcatca
    15541 ataagtttac ccacgcccat ggattcatct gtaggaatgt tcagtttttt gcaaacatct
    15601 ctcagggcag tttcatccat tccgttaacg tcaaacccgg tatattcctt gatggcgtca
    15661 taaagtgcta ttcgtttgta cggtgctttg aaactgatga tattgttgcc taccggcaaa
    15721 tctgttgtac cattggatgc cagagcaact ttttcgagta gcttttctgt aaaatccatc
    15781 atccagaaat aatctttgta ggcaacgtag aattcgagaa ccgtaaactc cggattgtgt
    15841 gttctatcca taccttcatt cctgaaattt ctcgaaaatt catacacaaa atcaaatccg
    15901 cctactatca gccttttcag ataaagttca ttagcgatac gaagataaaa cggaacatca
    15961 agcgcattgt ggtgagtaat aaatggcctg gcagctgccc cacccggaat gttctgcaac
    16021 acaggagtat ctacttcgat gccaccgtgt tcgttcaaat attctctgat ggcattcacc
    16081 atttttgtcc ttttaaggaa tacctccttc acaccctgat tgaccgtcag atcaacataa
    16141 cgctgtctgt aacgaaactc cgggtcactt accgcgtcaa agacattccc ttcagcatcg
    16201 gttttgacta ccggaagcgg tttgagggat tttgaaagca atgtaaaaga agtgacgtga
    16261 atggtagttt cacctgtttt tgttttgaat gcaaatcctt tgacaccaat ataatcacca
    16321 atgtcaagca attttttaat aagctgatcg aaaaaagtat aatcatcatt ttcagacaat
    16381 tcctcgcggc gaaggtagag ctgaatacgg ccatcagcat cctgaatatt cataaacatg
    16441 gccttaccgg cttcgcgcat attcatcagg cgcccggcca gacagacatc ctggaagttg
    16501 aggcggttct tggtaccatc ttccagaatt tcttctttgt aattttcttt tatttcagaa
    16561 gacaaatgtg tcaccggata taatgctgcc ggataagggt caatacccag ttcacggatt
    16621 ttatcaagtg attcacgtct tagtatttcc tgttcagtaa gttcgttatg acccatgcaa
    16681 tattgtttgt aagaataatg gctgcaaaat taggctttgt tttaataaat ttaaaggtaa
    16741 atagagaaac aaattgtaat aacaatttaa ctgaccaatt catacaacga atttggctat
    16801 tgtagtacct ttatgactgc aaacataact atctatgaga atgaggtttt tgtaccacaa
    16861 agtatgtatt tttactatcc tgaacatatt ttttattaca caactaacat cacaatgtgc
    16921 tgaaataacc gcaagcagca tcaatgggcc ttgtacgaat ggaagtacac cgtgcgatgt
    16981 atgcccggga gcagtgctca cattaaaagc ggaaggagcc aacctaataa ataatggctg
    17041 cgtggattgg tattacagcg acgatcaaaa ttttgaccct taccagaacg aaggcacatt
    17101 tattgcctgc ggaaagatta cgactacttt ccctccattc tgtgctgtat gtcctaccat
    17161 actggccatg atgatagacg cttgcgggcc ggagggaccg aacgaattca tgttattatc
    17221 atccggaagt gggtttaacc tcaacaacct acagattgat tttgctgtgc aaaacaacca
    17281 aatgggtaat aataataatg acatcaacat aggggcatca ccatgcggct ggaagactcc
    17341 taatgcatca ctgattgcca tgatactggc atccgccaac tgtactgcgg ccaatgtaat
    17401 ccctgtcgga ccgggcgaaa gcgtaccccc aaacggtttg gtggttgtat ttacaagtgg
    17461 tgacgaattt tctaattatg acttttcagc cttatgtgca acaggtcaaa aattatacgt
    17521 ggttcaaaat gcttgtaacc gtacagttgg agcattttct aatggttcaa gttccggttc
    17581 gagaacgacc accatttcta tcgcaggctg cagttgtacc aattcactta catatacttg
    17641 tgaagacctg gcacctgctc aggacggtga ctatgtgctg gcagatggta cacttggtaa
    17701 tgatggctgc accgctccgg atgtgatagc accacctgtt atgtcaacaa gtaccatagc
    17761 cgatgtgaca ctagccacaa ccgaagcaat gtgcaatatg ggtccgtact atattaaagg
    17821 aatcctgaat cctcctgccg atccattatg cccggatgtt tataccggta cttttgcgtt
    17881 taatgtcgtc tgtccggagg cagaagccat ggcttccagt ccaacttgtt ggggcggatc
    17941 agtcaatctt gaagcaaacg gaggcgacac ttataaatgg tcaggaccag gaggcttcaa
    18001 ctctatacaa cagaatcctg tcatcacagg actcaataac ctcaaatcgg ggacatacac
    18061 tgttaccgtt acaaatgcgg caggttgtac agatgtggct tcagtacagg ttactgttaa
    18121 tccggatatt accgtaagca tcacaccagc taatcctgca ttttgtgaag gcgaatccgt
    18181 tacccttaca ggaaatgcaa ccggtggctc aggaaatttt gattttgaat ggaccgaacc
    18241 aggaggtggt atggtaaatg gccagactat ttctgcttcg gtgcccggca cttatacgct
    18301 tgttgttaca gacggtgaag gatgcaccaa atcagcaagc atcaatgtca tacagaatcc
    18361 ttcccctgat gttgacatca cccctatgcc tgccaaagtt tgtgaaggtc aaacaaagac
    18421 cattactgcc aatgcaacag gtggttcacc ggcatataat tactcctgga gcaacggcat
    18481 gtcttcagct gccatcacgg taggtatagg cacttataat gtgaccgtca cggacgccaa
    18541 aggttgtaaa ggatttgcta acattgaagt tgaaaactat gacccgatag acattacatt
    18601 tatacaaaat cctgatgtat tgtgcaatgg agcttctgcc actcttacag ccaacgcgac
    18661 aggcggcaca gggacattca ccagttatca atggacaacg ccgggtggac ctgcatcggg
    18721 ttcaagtatt actgtcagtc aggcaggcaa ctataccgtt acaataacag acaatgcagc
    18781 ttgtaccgga acagcagtgc acaccataat cataaacccg atactgaatg tgagcatcaa
    18841 ccctaatcct gcttcttttt gtgcaggtgg tagtgtagat cttaccgcaa acgctacagg
    18901 aggatcagga tccggttatt tgtataactg gtcaactcct tcaggcaata aaaccggtca
    18961 ggtggtcact gcaacattag ccggatctta ttccgtgacc gttacggatg gagaaggctg
    19021 tacaggtact gcggtggtca atgtaacgga aaatgccaac ctgtcagtaa atattcttcc
    19081 tgcaacacca agtttttgcc ctggcagttt tgtcaatctg actgcaacac ctagcggagg
    19141 cacaactcca tacagctttt cgtggacaac accgggtggt actaaatcag ggcaggacat
    19201 tcaggcgaat attgcaggaa actacaccgt cacagtttct gacagcaagg gttgcacagg
    19261 aacagctaca gtaaatgtta ccgaatctcc gggatttccg gttaacatca cccctcctaa
    19321 cccaagtatc tgtccgggcg gaaaaattga tctgacagca gtaccgggtg gaggaaatgg
    19381 cacatttacc tatgcttgga caactcctgg cggcaataaa acaggggcga ccgtaactgc
    19441 aactgtcgtt ggcacatata atgtaacggt aaccgatagc ggcggatgca gtggtacagc
    19501 acaaacaacc gtggtcgaaa acccaaattt ggccgttagc attctgcctg ctgcaccaac
    19561 tttttgccct ggcgaaacgg ttgatctgac agtaaatgca cccggaaata ttgcttctta
    19621 tagttggact acacctggtg gatctaagtc gggctcgcct ctgaccgtta agactgccgg
    19681 caattatact gtaactgtta ctgacacagg tggttgcagt ggtacagcaa ctgtaaatgt
    19741 gtctgaaaat ccggcaaaga ccgtgaccat atctcctgct aatccaggtt tctgctcagg
    19801 gagtagtgta gaattgacag taaacgctac tggcaacaac ctcgtttaca actggactac
    19861 accttcaggc acggccaatg gcaatccact gatagcaaat gttgctggaa tatatactgt
    19921 tactgtgaca gaacagggtg cttgttcagg cacggcatct gttcaagttg atgaagtgag
    19981 tggcctgaca gttgacatta atcctaaacc ggcgagtttt tgtaataacg gatctgtttt
    20041 gcttacagca tcttcttcaa acggcatcgc accatttaca tacagctggt cttcacctgc
    20101 aggtaatgga acaggcatga catttaatgc aaatatagcc ggcaattata ctgtaactat
    20161 tactgacagc aagggttgtt cgggtagctt tactattccg gtagttcagt cgaacagcct
    20221 tattgttaca tttaatccgg tacttccggg tttctgtcca ggcaaaacgg ttgacataag
    20281 tacgaatacc attggtggtc aggctccgtt ctcctaccaa tggagttcac cattgggtac
    20341 atctgccaat ccggttatta ctaccggtac acccggcaac tacaaggtaa cggtaacaga
    20401 cagtaatgga tgctcgggtc tcggcgaagt ggttgttgac caattctcag aaccgatact
    20461 gacacttcct tccgcaccgg gattctgtcc gggtaacaat gttgcacttg atgttactgt
    20521 caatgacaat acaccaccat acatttatac ctggaccaca ccttcaggta attttaacac
    20581 caaaaccatt actgcaaata tagccggtaa ttatactatc tcagtaacaa atgccaacaa
    20641 ctgtactgcc agccaaagtg taaatgtcac agaatatcct gcgcctatag ctgaaatcat
    20701 tcctgacccg gtagtatttt gtaaaaatag ttcagcaacg gtgaccgcga atccatcagg
    20761 tggtgctgct ccttacactt ttctatggag tggtccggct accaatacta cagctacaaa
    20821 tattcagata aatactgccg gtgtatatac actactcgtc actgacgccc acagttgcac
    20881 agcagattat actttccctg ccaatgaaca accgggcttg ctggtaagcc tttctacaga
    20941 tcctcaaaaa ttgtgtggtg tcgctcagtt taatgttaat gccaacgtca ccggaggcaa
    21001 tccgccttat acctattcat ggaatacacc cggtggcacc caaacaggta gtactatatc
    21061 gggcaatgaa tcgggtacat actctgtgac tgtaacggac aatgaagctt gcagcggtgt
    21121 cgctgattta cttataaaca acaaaccatt aaatgcattg gtcacgcctg ttgaccctac
    21181 ttgtaaaagc cctgccggag gtagtattgc acttgtattc gatacgggca ttgctctacc
    21241 tgcaaatatc aatcttaata acaatagtgc aggtcaaatc acacagacca ctcacaccat
    21301 caacggtttg gccccgggca attatcagtt attggttaca gatgccaaag gctgcgaatt
    21361 tcagacaaca gtgacgcttg ctccattacc tgtattgcac cttgaccttg ggcaagatgt
    21421 taccatcaac cttggtgaca cctacaccat tcatccgata acggactttg ctatcaacaa
    21481 cataagctgg accaacgaac aaagtctgaa ctgtcccgat ccgtgcatca atcctgttgc
    21541 gggccctgtc aacaccacta cctacaaagc aagtgcttat gatgaaaatg gctgtcttgc
    21601 tgaagatgaa ctaactattt atgtacatat aaaaacaacc gtatatgttc caaacagctt
    21661 cagcccggat gccgatggta tcaacgacaa atgggtaata tttgcagaca atacggtaaa
    21721 gctgattaaa acattgcata tctacgaccg atggggcgaa tctatcatgc agcaggagaa
    21781 ttttccacct aatgacccag actacggttg gaacggaact ttcagacgaa aaccactcga
    21841 tccggcagtt tttgtgtatt attttgttgt cgaatatgaa aacggaacga ccaaagtatt
    21901 taaaggtgat ataaatatca taagtaaaaa atgacagaag attatataga aataaaccgc
    21961 aaactctgga acgacaaggt gggcatacat gttgattcag aattttatga ccaacaaagt
    22021 tttcttgccg ggaaatcgag tctgaaacgt ccagaactcg atctgcttgg cgacatcaca
    22081 ggaaaaaaaa tacttcatct tcagtgtcat ttcggacagg atactatgtc tctttccagg
    22141 atgggtgggc atgctaccgg tatcgatttc tctgaaaaag ctatcgaaat agcaaatgaa
    22201 acgacaaagt cactaagctt gggtacatcc tttgtatgtt gtgatgtata tcatactcct
    22261 gcatttattg accaacagtt cgatatcgtg tttacaagct atggcaccat cggctggcta
    22321 cccgatctgc aaaaatgggc ttctgtgatt tacaaaatgc tcaaacctgg cggaaagttg
    22381 atcttcgttg aatttcatcc atttgtcttt acgcttgatg aagaatttga tttcgtgaaa
    22441 tatttctatt tcaataagga agaaattgta gaaacagaag aagggacgta tgctgaaaaa
    22501 aatgcacaaa tctcaaataa aagcatcagc tggaaccacc cgataagcga agtactccaa
    22561 aacctgatag atgccggact aacaatatca agatttgaag aatacgacta ttcaccctat
    22621 gcctgcttca gtaaacttgt aaaacaaggt gaagacgaat acagagtaga aggtcttgaa
    22681 ggcaaactgc ctatggtgta tgctattgtt gctaataaag ctgaataata gaatccttct
    22741 cgttttatca attttgaatt ttatcaccaa agctaagatc acctgcatcg cccagacctg
    22801 ggacgatgta agacttagct gttagttctt catcaacatc gccgagccaa agtgtagcat
    22861 tgggcagatg acggcgcacg tggtcaactc cttccctggc tgcaatggca gacacgatgt
    22921 gtacagactt tgggtggcca tatctggtaa gctcctgaag tgtccgtgct atagactgac
    22981 cggtggcgag cattgggtca gctataatga gtaccgcatc attcagatca ggacacgtga
    23041 catattggag acttatctcg aatgaaccgt ccttatggtg cttgcggtaa gctgatataa
    23101 aagcattgcc ggcatagtca aaataattga gcataccctg gtgcatcggt agtcctgcac
    23161 ggagtatggt tgcaagtact acttttcgct ctgtctttct taccatcgct gtaccaagcg
    23221 gtgttgtcac ctcctgttcg taaaagtcaa gcgttttact tatttcgtag gcgaatatt
//
LOCUS       DAOQQY010000015        22962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_666_length_22962_cov_5.740647, whole genome shotgun sequence.
ACCESSION   DAOQQY010000015 DAOQQY010000000
VERSION     DAOQQY010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22962
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_22962_cov_5.740647"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1966
                     /gene="fusA"
                     /locus_tag="PLE23_01705"
     CDS             <1..1966
                     /gene="fusA"
                     /locus_tag="PLE23_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMX82010.1"
                     /translation="RGITITSAATKCNWEVEGQKFDLNIIDTPGHVDFTVEVSRSLRI
                     LDGLVFLFSAVDGVEPQSETNWRLADSFKVPRIGFVNKMDRSGADFFNVVQDMKLKLG
                     ANAIPLQVPIGAEDSFKGVVDLISNQAIVWNEHDHGMTYEVITIPEDLLDIVHEKRAQ
                     LIEEVASYDDTLMEKFFEDPNSITIPELRKAIRAAVCDMKFTPVMCGSSFKNKGVQAM
                     LNAVCYYLPSPLDVDAIEGINPKTDEPTSRKPSVNEPFTALAFKIATDPFVGRLCFIR
                     AYSGALDAGSYVLNTRTDSKERISRLYQMHANKQNPIDRVEAGDICAAVGFKDIRTGD
                     TLCDEKAPIVLESMVFPEPVIGIAIEPKTQKDLDKLGMSLAKLAEEDPTFKVQYNEDT
                     NQTVISGMGELHLEVIVDRLRREFKVECNQGAPQVNYKEALTKSVKHRERLKKQSGGA
                     GLFADMEFELGPADQEFLDSEEFKSGKKKLQFVWDIVGGAIDRNYQKPINDGFTAMMN
                     NGVLAGYGIDAMKVRVYDGSMHAVDSKPIAFELCAKEGFRAAASSAAPVLLEPIMKLE
                     VTCPDEYTGGVIGDLNRRRGLIKGQDAKFSAVIIKAEVPLSEMFGYVTQLRTITSGRA
                     SSTMEFSHYNPAPSGIADSVIEKSKGAVKV"
     gene            2039..2347
                     /gene="rpsJ"
                     /locus_tag="PLE23_01710"
     CDS             2039..2347
                     /gene="rpsJ"
                     /locus_tag="PLE23_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HMX82011.1"
                     /translation="MNQKIRIKLRSYDHNLVDKSTEKIVKTVRSSGAVVSGPIPLPTE
                     KKLFTVLRSPHVNKKAREQFQLRTHKRLIEIYTPTQKTVDALSKLELPSGVDIQLKLT
                     "
     gene            2420..3040
                     /gene="rplC"
                     /locus_tag="PLE23_01715"
     CDS             2420..3040
                     /gene="rplC"
                     /locus_tag="PLE23_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HMX82012.1"
                     /translation="MNGLIGKKVGMTSIYDASGKNIACTVIEMGPNVVTQVKSTDSDG
                     YNALQIAYGEAKAKRTTKALTGHFEKAGTTPKKKLIEIKDPKISKNLGEEINIEEYFK
                     VGDTIKAVGTSKGKGFQGVVKRHNFGGVGQQTHGQHNRLRAPGSVGASSYPSKVFKGM
                     RMAGRMGGDQVKIKNLKVVNIFPEKNLIVIKGAIPGHKGSFVIIEK"
     gene            3047..3676
                     /gene="rplD"
                     /locus_tag="PLE23_01720"
     CDS             3047..3676
                     /gene="rplD"
                     /locus_tag="PLE23_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HMX82013.1"
                     /translation="MKLDVFNINGVSTGRTIELPEEIFGVTPNQQCVYLDVKQYLANQ
                     RQGTHKAKERSELSGSTRKLHKQKGTGGSRKGDIKNPLFHGGATIFGPRPRDYSFKLN
                     KKVKALARKSALSAKFSEGSIMIVEDFTFDAPKTKGFLNVIDSLKVNGKRSLFVLPGA
                     DKNVYLSARNIPRTMVMRAEDINTYQIVKSASVIISESSINKIKETLQG"
     gene            3683..3979
                     /gene="rplW"
                     /locus_tag="PLE23_01725"
     CDS             3683..3979
                     /gene="rplW"
                     /locus_tag="PLE23_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HMX82014.1"
                     /translation="MSKNILIKPLITEKTEQLTTKRSQYTFVVDKKSNKIEIKNAVEK
                     MYSVNVESVNTAIMPTKYRTRNTKAGLIKGRVSGVKKAVITLADGEQIDYFGEV"
     gene            4002..4838
                     /gene="rplB"
                     /locus_tag="PLE23_01730"
     CDS             4002..4838
                     /gene="rplB"
                     /locus_tag="PLE23_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HMX82015.1"
                     /translation="MAVKKFKPITPGTRFRIGNSYAEVTTDTPEKSLVTGINKSGGRN
                     SAGAMTVRQRGGGHKRLYRIIDFKRDKENIPARVKSIEYDPNRSAFIALLAYNDGEKR
                     YIIAPNTLKVGDVVNSGKGVAPNTGNALYLSEIPLGSFIYAIEMTPGKGASIARSAGT
                     NGQLMGREGKYAVVKMPSGEVRRILLTCKATMGATSNSDHALTVMGKAGKNRHAGRRP
                     RVRGVAMNPVDHPMGGGEGRASGGHPRSRTGIMAKGQKTRNTKKPSSKLIISRRKTSG
                     RK"
     gene            4854..5120
                     /gene="rpsS"
                     /locus_tag="PLE23_01735"
     CDS             4854..5120
                     /gene="rpsS"
                     /locus_tag="PLE23_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HMX82016.1"
                     /translation="MARSLKKGPYVFHKLLDKVKAAKENNKKAVIKTWSRSSMIIPVM
                     VGETIAVHNGKTFVPVYVTENMVGHKLGEFSPTRLFRGHGGKKK"
     gene            5150..5539
                     /gene="rplV"
                     /locus_tag="PLE23_01740"
     CDS             5150..5539
                     /gene="rplV"
                     /locus_tag="PLE23_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HMX82017.1"
                     /translation="MKAVAKLKNCPMSPRKMRLVVDNIRGKKVEDALNILKFTNKEAS
                     AWLTKVLVSAVANWEVKNGGSVSADDYALIVKTAFVDGGTMLKRFQPAPQGRAHRIRK
                     RTNHITIEVENRIPTPMDSEMAAESAE"
     gene            5575..6318
                     /gene="rpsC"
                     /locus_tag="PLE23_01745"
     CDS             5575..6318
                     /gene="rpsC"
                     /locus_tag="PLE23_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HMX82018.1"
                     /translation="MGQKANPIGNRLGIIRGWESNWYGGRNFADNVVEDEKIRIYLNA
                     RIPKGGIAHIIIERTLKRITVTIHTSRPGIVIGKGGNEVDRIKEELKKLTKKDVQINI
                     VEIRKPELDATIVGDTIAKQLESRINYRRAIKMAIQSTIRAGAEGIKVRISGRLNGAE
                     MARSEEYKEGRTPLHTFRADIDYAISEALTVYGKIGIKTWICKGEVLGKRDLSFTTYE
                     EKKDTKDQRKRPGGEGRERGGQQRPPKRK"
     gene            6409..6825
                     /gene="rplP"
                     /locus_tag="PLE23_01750"
     CDS             6409..6825
                     /gene="rplP"
                     /locus_tag="PLE23_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HMX82019.1"
                     /translation="MLQPKRTKYRKQQKGRIADITYRGAIVSFGSFGLKSVQSGRITS
                     RQIESARIAMTRYMKREGQVWIRIFPDKPITSKPAEVRMGKGKGALDHYVANVEAGRI
                     MFEIDGVPADVAVEALRLAAQKLPLLTKTVTRPDLG"
     gene            6839..7099
                     /gene="rpmC"
                     /locus_tag="PLE23_01755"
     CDS             6839..7099
                     /gene="rpmC"
                     /locus_tag="PLE23_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HMX82020.1"
                     /translation="MATNKYQELINFSDADLATELQVTEREYKRMQFDQALKGLESPI
                     KLREVRRDIARIKTEIRRRELSGMSEAALASRNKIVNRRRKS"
     gene            7120..7377
                     /gene="rpsQ"
                     /locus_tag="PLE23_01760"
     CDS             7120..7377
                     /gene="rpsQ"
                     /locus_tag="PLE23_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HMX82021.1"
                     /translation="MVERKIRKQKTGVVSSNKMDKSITVLVLRRLRHPKYGKFVKKTN
                     KFMAHDENNECLIGDKVRIMETRPLSKNKRWRLVEVIEKVK"
     gene            7398..7763
                     /gene="rplN"
                     /locus_tag="PLE23_01765"
     CDS             7398..7763
                     /gene="rplN"
                     /locus_tag="PLE23_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HMX82022.1"
                     /translation="MIQQESRLKVADNSGAKEVLCIRVLGGTKRRYASIGDKIVVSIK
                     EAVPGGVKKGTVSKAVIVRTRKEIRRDDGSYIRFDDNAVVLLNNSDEPRGSRIFGPVA
                     RELRDRNYMRIVSLAPEVL"
     gene            7782..8123
                     /gene="rplX"
                     /locus_tag="PLE23_01770"
     CDS             7782..8123
                     /gene="rplX"
                     /locus_tag="PLE23_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HMX82023.1"
                     /translation="MATKQTQKRFAPKLKIKKDDKVIVLSGDDKGKRGKVLSVDPTKN
                     RAIVEGVNIVKKHTKPGNNKPGGINEIEAPVNLSNLSLIDPKSGEATRVGRKLVDGKM
                     VRYSKKSGEII"
     gene            8128..8685
                     /gene="rplE"
                     /locus_tag="PLE23_01775"
     CDS             8128..8685
                     /gene="rplE"
                     /locus_tag="PLE23_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HMX82024.1"
                     /translation="MSYESLHKKKYRDEIVSQLMEQFQYKTVMQVPKLVKVTINQGLG
                     GATSDKKLVENALQEIALITGQKPVPTKAKNSISNFKLREGMVIGAKVTLRNERMWEF
                     IDRLLTIALPRIRDFRGINNKSFDGRGNYSLGITEQIIFPEIDIDKVNKITGMDINFV
                     TTAKTDAEAFALLKLLGFPFKNQKN"
     gene            8693..8962
                     /gene="rpsN"
                     /locus_tag="PLE23_01780"
     CDS             8693..8962
                     /gene="rpsN"
                     /locus_tag="PLE23_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HMX82025.1"
                     /translation="MAKKSIIAREVKRKKLVEKYAELRKQLKEANDYDALDKLPRNSN
                     PIRMHNRCRLTGRPKGFMRDFGLCRNMFRKMALDGKIPGITKSTW"
     gene            9001..9405
                     /gene="rpsH"
                     /locus_tag="PLE23_01785"
     CDS             9001..9405
                     /gene="rpsH"
                     /locus_tag="PLE23_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013858092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMX82026.1"
                     /translation="MAVTDPIADYLTRIRNAQMANHKIVEIPSSRMKRSITEILYEQG
                     YIEKYKFDTTDPIQPIIKIALKYNPDTKLPVIRELGRISRPGLRKFSGSKDIPRIING
                     LGLCIVSTSKGVITDKEARKLNVGGELVCYVY"
     gene            9426..9983
                     /gene="rplF"
                     /locus_tag="PLE23_01790"
     CDS             9426..9983
                     /gene="rplF"
                     /locus_tag="PLE23_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMX82027.1"
                     /translation="MSRIGKAIIRLPEKVEVSWTKGNLVTVKGPKGQLNQQIDPDLSI
                     NLEDGVLSLSRPTDQKRHRSVHGLYRALLNNMVIGVSEGYERQMELNGVGYRVTNTNQ
                     MLELALGYSHPIHFALPAEVALETLTEKGSPPKIILKSIDKQLIGQVAAKIRAMRKPE
                     PYKGKGIKFVGEIIRRKAGKTSGKK"
     gene            10006..10356
                     /gene="rplR"
                     /locus_tag="PLE23_01795"
     CDS             10006..10356
                     /gene="rplR"
                     /locus_tag="PLE23_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMX82028.1"
                     /translation="MKRTKEEKRRRIHYRIRKKISGTAETPRLSVYKSNTAIYCQLID
                     DVNGHTLAAASSKEANASSGTKSEQAKAVGALIAEKAKSANILSVAFDRGGYLYHGRI
                     KALADGAREGGLQF"
     gene            10379..10897
                     /gene="rpsE"
                     /locus_tag="PLE23_01800"
     CDS             10379..10897
                     /gene="rpsE"
                     /locus_tag="PLE23_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMX82029.1"
                     /translation="MAKKVVQNVNPAESELKDRMVTLNRVAKVTKGGRTFSFAAIVVV
                     GDGNGIVGQGLGKAREVTDAIQKAIDDAKKNLVKVPVYKGTIPHEQNGKFGAGRVLIK
                     PSAHGTGVIAGGAMRAVLEMAGINDVLAKSLGSSNPHNVVKATIDALSKLRSPIDVSM
                     QRSIKLTKVFEG"
     gene            10932..11111
                     /gene="rpmD"
                     /locus_tag="PLE23_01805"
     CDS             10932..11111
                     /gene="rpmD"
                     /locus_tag="PLE23_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMX82030.1"
                     /translation="MTKLKLTQVKSVIDRPKDQKFTMRSLGLKKVYHSVEIDATPSNV
                     GMVEKVKHLLKVEKV"
     gene            11140..11586
                     /gene="rplO"
                     /locus_tag="PLE23_01810"
     CDS             11140..11586
                     /gene="rplO"
                     /locus_tag="PLE23_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HMX82031.1"
                     /translation="MKLHTLRPAKGAVKKSKRIARGQGSGHGGTATRGHKGAGSRSGN
                     SIKRGFEGGQMPLQRRIPKRGFKNINRIDYVVVNLSTLQNLSDKYNVSSFDIDFFVNH
                     GIAKSSEKVKVLANGTLKSKISVVANAASEKAKQTIEQLGGSINLV"
     gene            11596..12969
                     /gene="secY"
                     /locus_tag="PLE23_01815"
     CDS             11596..12969
                     /gene="secY"
                     /locus_tag="PLE23_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMX82032.1"
                     /translation="MKSLITTLKNIFSIKELKDRILYTLLLLLIFRIGTEIVLPGVRA
                     DVLKSATNANANDLLGLLNIFTGGAFNNASILALGIMPYITASIVVQLLGFAVPYFQR
                     LQSKEGESGRKKLNQITRLLTVAITLVQGGAYLTYVNSLGAVDTKIPTGIFWLCNSII
                     LSTGTVFSMWLGERITDKGIGNGISLLIQMGIIARLPQAFAFEFSSQASNNNLLIFIL
                     ELALLFAVIMLTILVVQGVRKIPVTFANRMVGRTSKYMPGEGARDYIPLKLNAAGVMP
                     IIFAQALMFLPATVAQFATKDQSALASSSIFSALNDFMSIPYNIIYFVMVVLFTYIYT
                     ALIVNSQQYTDYLKRQNAFIPGIKPGSHTNEFIDSITSRITLPGAIFLGIIAIFPAIA
                     GRVLGINHSFAVFFGGTSLLILVGVALDTLQMIESYLLMRKYDGLVESGKIQGRSSSG
                     INIGAGY"
     gene            12992..13819
                     /gene="map"
                     /locus_tag="PLE23_01820"
     CDS             12992..13819
                     /gene="map"
                     /locus_tag="PLE23_01820"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMX82033.1"
                     /translation="MIFYKTDEEIELIRQSNLIVSGALAAVAAELRPGITGLKIDQIA
                     ETYIRDQKAEPGFKGYNGFPASLCISVNNQVVHGIPSNIPFKEGDIVSVDCGVQKNGF
                     FGDSAYSFGLGILSHEHKRLLEVTLESLYLGIEKATVGNRIGDIGYAIQSYVENNGYS
                     VVRDLIGHGVGRDLHEPPEVPNFGRPGKGTMLQEGLVIAIEPMINLGKKEVRVGEDGW
                     TVITKDLLPSAHFEHSIAVRKGGVDILSDYSIIEEEIKKNKNIEQLSIKIPIFAPRN"
     gene            13825..14043
                     /gene="infA"
                     /locus_tag="PLE23_01825"
     CDS             13825..14043
                     /gene="infA"
                     /locus_tag="PLE23_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMX82034.1"
                     /translation="MAKKDLIQQDGIIEEALSNAMFRVRLENNHTILATISGKMRMNY
                     IRILPGDKVAIEMSPYDLSRGRIIYRYK"
     gene            14069..14185
                     /gene="rpmJ"
                     /locus_tag="PLE23_01830"
     CDS             14069..14185
                     /gene="rpmJ"
                     /locus_tag="PLE23_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HMX82035.1"
                     /translation="MKVRASIKVRTADCKIVKRNGKLYIINKKNPKFKQRQG"
     gene            14216..14593
                     /gene="rpsM"
                     /locus_tag="PLE23_01835"
     CDS             14216..14593
                     /gene="rpsM"
                     /locus_tag="PLE23_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMX82036.1"
                     /translation="MARIAGIDLPKNKRGIIGLTYIYGIGPNISRDILSQAEIDESVK
                     VADWNDEQIKKITSIIQNQYKVEGELRSEIQLNIKRLMDIACYRGLRHRKGLPLRGQS
                     TQTNARTRKGKRKTVANKKKVTK"
     gene            14623..15012
                     /gene="rpsK"
                     /locus_tag="PLE23_01840"
     CDS             14623..15012
                     /gene="rpsK"
                     /locus_tag="PLE23_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMX82037.1"
                     /translation="MAKAVKKVKKRKVRIDSDGLVFVKASFNNLIVSLCNRQGQVISW
                     SSAGKVGFRGSKKNTPFAAQKAAGEASLTAYEAGMRTANVYVKGPGAGREAAIRAVDQ
                     AGIKVLRIFDVTPVPHNGCRPPKRRRV"
     gene            15034..15645
                     /gene="rpsD"
                     /locus_tag="PLE23_01845"
     CDS             15034..15645
                     /gene="rpsD"
                     /locus_tag="PLE23_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMX82038.1"
                     /translation="MARYTGPKTKKSRSFGEAIFGFDKAFEKKKYPPGVHGLKRKKKQ
                     KSDYSMQLTEKQKAKYTYGLLERQFRNLFVKAAAKSGVTGEILLQFLEGRLDNVVYRL
                     GISPSRPGARQLVTHKHILVNGKLANIPSMLLQPGDVISVRGKSQGHDFITNNVRGKN
                     EVKKYAWLEWNPDKMAGTYIQAPSRDQIPENINEQLIVELYSK"
     gene            15741..16739
                     /locus_tag="PLE23_01850"
     CDS             15741..16739
                     /locus_tag="PLE23_01850"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HMX82039.1"
                     /translation="MSILNFQKPDKILLQKATDFEGVFEFKPLEPGFGQTVGNSLRRV
                     LLSSLEGVAISAVRIAGVDHEFSTIKGVVEDTVEIILNLKQVNLKQVLRDEDITSEKI
                     YVTLSGRTEFRAGDLEDFTNVFKVMNPDLLICRMEPSVTLEMEFTITKGRGYVPADEN
                     LPKDASIGVMPIDAIYTPIKNVAYKVSNTRVGQRTDYEKLTIEITTDGTIHPEDAIKD
                     ASRILIQHLMLITDENITFEDATSREDNIVDEHILHMRKLLKTSLEDLDLSVRAYNCL
                     KAAKINTLGELVRFDMNELLKFRNFGKKSLVEIDELLQEKGLTFGMDLSKYRLDED"
     gene            16814..17164
                     /gene="rplQ"
                     /locus_tag="PLE23_01855"
                     /pseudo
     CDS             16814..17164
                     /gene="rplQ"
                     /locus_tag="PLE23_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            17538..18644
                     /gene="carA"
                     /locus_tag="PLE23_01860"
     CDS             17538..18644
                     /gene="carA"
                     /locus_tag="PLE23_01860"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMX82040.1"
                     /translation="MQAGSDRKAILLLEDGTVFEGRAAGKIGTATGEIAFSTGMTGYQ
                     EVFTDPSFYGQLLVATNVHIGNYGIRYPESESGSIKINGFICRNFTPSYSRQMADDSI
                     QEYFEKENLVAISGIDTRSLVKHIREKGAMNGIISSEILDVAQLKSTLAIVPSMDGLE
                     LASIVSTKEPYDYGSPEAKYRVAVLDFGIKENILRCLAQRDCFMRVFPAKTNFAELEQ
                     WNPDGYFLSNGPGDPAAMDYALETVREIVKTNKKVFGICLGHQLIGLSVGINTYKLHH
                     GHRGINHPVKNLITGRCEITSQNHGFGLDAKNIEQFKDVVEISHVNLNDNTIEGIRLK
                     NGNGFSVQYHPEAAPGPHDARYLFDDFIELLNKN"
     gene            18649..19938
                     /gene="eno"
                     /locus_tag="PLE23_01865"
     CDS             18649..19938
                     /gene="eno"
                     /locus_tag="PLE23_01865"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMX82041.1"
                     /translation="MSAIIDINAREILDSRGNPTIEVDVITEGGGYGRAAVPSGASTG
                     KHEAVELRDGDTKRFGGKGVLNAVQFVEDVISPELIGLDVFEQREIDQTMIQLDGTPN
                     KSKLGANAILGVSLAAAKAASSESDMPLYRYIGGVNSFTLPVPMMNILNGGSHADNKI
                     DFQEFMIMPVGADTFREGLRMGVEIFHSLKKVLSAKSYSTNVGDEGGFAPNIQSNEES
                     IELVLQAIEKAGYKPGDEVLIAMDAAASEFYDENKKIYDFHKSGSKPLTGEEMVDFWV
                     KWASKYPIFSIEDGLHEDDWDSWAMLTQKLGSKIQLVGDDLFVTNSERLRRGIENNIA
                     NSVLVKVNQIGTLSETIDTVTMAHRNRYTTVISHRSGETEDTTIADLAVALNAGQIKT
                     GSASRSDRVAKYNQLLRIEEELGTSAYYPGKNLLKRL"
     gene            19962..20267
                     /locus_tag="PLE23_01870"
     CDS             19962..20267
                     /locus_tag="PLE23_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HMX82042.1"
                     /translation="MEKIKEITDKIPKWLFNKFAITTVLFIFWMVFLDKYNLFTQFGM
                     MSDIRKLKQEKLMYAEKIEKLKAEKEAFEHNLEKYAREKYLMHKSNEDVYIVLDKSK"
     gene            20273..21142
                     /gene="sucD"
                     /locus_tag="PLE23_01875"
     CDS             20273..21142
                     /gene="sucD"
                     /locus_tag="PLE23_01875"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMX82043.1"
                     /translation="MSILVNKNSKVIVQGFTGNEGTFHAEQMIAYGTNVVGGVVPGKG
                     GQQHLGRPVFNTVADAVREAGADVSIIFVPPAFAADAIMEAAQSGIKVIVCITEGIPV
                     QDMLKAKDYIQRFDCRLIGPNCPGIITADEAKIGIMPGFVFKKGKIGIVSKSGTLTYE
                     AADQVVKAGLGISTAIGIGGDPIIGTTTREAVEMFMNDPETKGIVMIGEIGGSLEAEA
                     ANWIKANGNKKPVVGFIAGQTAPKGRKMGHAGAIIGGADDTAEAKMKIMSECGIHVVS
                     SPAEIGDTMRKLV"
     gene            complement(21230..>22962)
                     /locus_tag="PLE23_01880"
     CDS             complement(21230..>22962)
                     /locus_tag="PLE23_01880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMX82044.1"
                     /translation="IEKYGYALYYQGHLTSQSPNFRNNDIQNTAAYYKNLINQRFQVL
                     GGRAEDPTGRVLEINKTGGGLIKILSLFSYIFVLLAVTFLFFIALNTFVKFIPGIYTF
                     ENQSNISLRNRIQVAIVSMIVVSFVAIGIITIIYFNFSNKKNLSENLIRKVTDISNRL
                     IDKNMKPDETDLVAFNEELESEIIIYDKKGYIAGSFPAKIETVQPELQYLVKHPMVMN
                     TFSQQQIGGFFNRLNGSYLVHTVLAPDSTVNGYIVMPFSHTDLSTNNQIADFIGALIN
                     TYVFLLLIASALALAVGRSITKPLLVLGDKLNRFKLGKQNEPISWSKNDEIGFLVGEY
                     NQMIVKLEHSAGLLARSEREEAWREMARQVAHEIKNPLTPMKLNVQYLLHSYHSDPES
                     REQRIKNICDTLVQQIENLSAIAGTFGEFAQEPKINSEDLHLNTIVNGIYELFEKTGN
                     EHLNFKKSILDEDIIVHADKSHLIRIINNLIKNAVQAIPSERIGRVEVLTRREGNKII
                     IAVKDNGHGIDDSKKEKIFMPNFTTKSSGSGLGLAICKKLIESMDGEIYFSTIPDKGS
                     TFFVRLPIKE"
BASE COUNT         7699 a         3994 c         4920 g         6349 t
ORIGIN      
        1 aagaggtatc actattacat ctgctgctac taaatgtaac tgggaagtcg aaggccaaaa
       61 gtttgacctg aatattatcg atactccggg tcacgtggat tttaccgttg aagtttcaag
      121 atccttacga atccttgatg gccttgtgtt tttgttctct gcagtagatg gtgtagagcc
      181 acagtctgaa accaactgga gacttgcaga tagcttcaaa gtaccccgta tcggttttgt
      241 aaacaaaatg gaccgttcag gggcggattt cttcaatgtc gttcaagata tgaagttaaa
      301 actcggtgct aatgccattc cactacaagt accaataggt gcagaagatt cattcaaagg
      361 tgtagtagat ttgatttcaa accaggctat tgtttggaat gaacacgatc atggtatgac
      421 ttacgaagtt attacaatac ctgaagattt attagacatc gtacatgaga aaagagcgca
      481 actgatagaa gaagttgctt cttatgatga tacattgatg gagaagttct ttgaagatcc
      541 gaactctatc actattccag agttgagaaa ggccatcaga gctgctgttt gtgatatgaa
      601 gttcactccg gttatgtgtg gatcttcatt caagaacaaa ggtgtacagg ctatgttgaa
      661 cgctgtttgt tattatcttc cgtcaccact tgatgtggat gctatcgaag gtatcaaccc
      721 taaaacagat gagccaactt caagaaaacc aagtgttaat gagccgttca ccgcacttgc
      781 attcaagatt gctactgacc catttgtagg gagactttgc tttatcagag catattcagg
      841 tgcacttgat gcaggatctt atgtactgaa tacaagaaca gacagtaaag aacgtatctc
      901 tcgtctttat caaatgcacg ctaataagca aaacccgatt gacagggtag aagcaggtga
      961 tatttgtgcg gcagttggtt ttaaagatat cagaacagga gatactttgt gtgacgagaa
     1021 agctcctatc gtattggaaa gtatggtttt ccctgaaccg gttatcggta tagctattga
     1081 gccaaaaact cagaaagatc ttgataaact tggtatgtct ctagccaaac tcgctgaaga
     1141 agacccaact ttcaaggtac aatataatga agatactaac cagactgtta tcagcggaat
     1201 gggtgaacta caccttgaag taatcgttga ccgtctaaga agagaattta aagttgaatg
     1261 taaccagggt gcgcctcagg ttaattataa agaagcactt acaaagagtg ttaaacaccg
     1321 cgaaagactt aaaaagcaat cgggtggtgc cggtctcttt gcagatatgg aattcgaact
     1381 cggacctgca gaccaggaat tccttgactc tgaagaattc aagtcaggaa agaaaaaatt
     1441 gcaatttgtt tgggatatag ttggtggtgc cattgataga aattatcaga aaccgatcaa
     1501 cgatggtttt acagcgatga tgaacaacgg tgtacttgcc ggatatggta tagatgccat
     1561 gaaggttaga gtatatgatg gttcaatgca cgcagttgac tctaagccta ttgcatttga
     1621 actttgtgct aaggaaggat ttagagcggc tgcttcatct gctgccccag tgcttcttga
     1681 gcctattatg aaacttgaag tgacatgccc tgatgaatat acaggtggtg ttatcggtga
     1741 cctaaacaga cgaagaggtc tgatcaaagg tcaggatgca aaattctctg ctgttattat
     1801 caaagccgaa gttccacttt ctgaaatgtt cggttatgtt actcagctca gaaccattac
     1861 ttcaggtcga gcatcatcaa ctatggaatt ctcacattac aatccagcac catcaggtat
     1921 tgcagattca gtgattgaga aatctaaagg tgcagttaaa gtatagtatt aaacattgat
     1981 taactggaaa atataaagta ttttccaata tttttgaaaa attttatttg attaggttat
     2041 gaatcaaaaa atcagaatta agctacgttc ttacgaccac aaccttgtgg acaagtcaac
     2101 tgaaaagatt gttaaaactg taagaagcag tggtgctgtt gtatctggtc cgattccgct
     2161 gccaactgag aagaaacttt ttactgttct gcgttccccg cacgtcaaca aaaaagctcg
     2221 agagcagttc cagctaagga cacacaaaag actcattgag atatacacac cgacgcagaa
     2281 aacagtggat gcactttcaa aattagagct tccaagtggt gtggatattc aactaaagtt
     2341 aacgtaattt aattcaaaac atttcccagc ctgagtattt caggactgga ttcatttgaa
     2401 cttttaaaac tatctaattg tgaacggatt gataggcaaa aaagtcggaa tgacgagtat
     2461 atatgacgcg tccggcaaaa atatagcatg tacagtaatt gaaatgggcc ccaatgtggt
     2521 tactcaggtc aagtcaactg atagtgatgg gtataatgca ctgcagattg cttatggcga
     2581 agctaaagcc aaacgtacaa ctaaggcatt aactggtcac tttgaaaaag caggtacaac
     2641 acctaaaaag aaactaatag aaatcaaaga tcctaagatt tcaaagaatc ttggtgaaga
     2701 gattaatatt gaagaatact tcaaggttgg tgatacaatt aaagcagtcg gcacatctaa
     2761 aggtaaaggc ttccaaggtg ttgtcaaaag acataatttc ggtggtgttg gtcagcagac
     2821 acacggacag cacaaccgtc taagagctcc tggttcagtt ggtgcttctt cttatccttc
     2881 taaagtattt aagggtatga gaatggccgg ccgtatggga ggtgatcaag taaaaattaa
     2941 aaacctgaaa gttgtaaata ttttccctga aaagaacctg attgtcatca aaggtgccat
     3001 accaggtcat aagggttcat ttgtaatcat tgaaaaataa tcggacatga agttggatgt
     3061 ctttaatatt aatggggttt ctacaggacg tacaatcgag ttgcctgaag aaatttttgg
     3121 agtaactcct aatcagcaat gcgtttacct tgatgttaag cagtatcttg caaatcaaag
     3181 acaaggtacg cataaggcta aagaacgctc ggagctttcg ggttctacac gtaagttgca
     3241 taagcaaaaa ggtaccggtg gttcaagaaa aggtgatata aaaaatcctt tgttccatgg
     3301 tggcgcaact atctttggcc caagacctcg ggattatagc ttcaaactta ataaaaaggt
     3361 aaaagcgctt gcaagaaaat ctgcattatc agctaaattt agcgaaggtt caattatgat
     3421 tgtcgaggac tttacatttg atgctccaaa gactaaaggt tttttgaatg tcattgatag
     3481 cttaaaggta aatggaaaaa gatctctttt tgtactacct ggagccgata agaatgttta
     3541 tttgtctgca agaaacatac ctaggacaat ggtaatgcgt gctgaagata tcaatacgta
     3601 ccagatagta aaatctgctt ctgttataat atctgaaagt tcaataaata aaattaaaga
     3661 aacgcttcaa ggttaaaata tcatgtcaaa gaacatttta ataaaacccc ttattacaga
     3721 aaaaactgag caacttacta ccaaaagatc tcagtacact tttgtggttg ataagaaatc
     3781 gaataaaatc gaaattaaaa atgctgtcga gaaaatgtac agtgttaatg tcgaatctgt
     3841 gaatactgca ataatgccta ctaaatacag aacaagaaac actaaagccg gattgataaa
     3901 aggtcgcgtc tctggtgtta agaaggctgt cattactctg gctgatggcg aacagataga
     3961 ctattttggt gaagtttaat aaattgtaaa taactgataa aatggcagtt aaaaaattta
     4021 aacccataac tcccggaaca cgcttccgta ttgggaattc ttatgctgaa gtcactacgg
     4081 atactcctga aaagagcctt gtgacaggta ttaacaaatc aggcggaagg aacagcgccg
     4141 gtgcgatgac agtacgtcaa cgtggtggtg gacataaacg tctttatcgt attattgact
     4201 tcaaaagaga taaggaaaat attcctgcaa gggttaagtc aatcgagtac gatccaaaca
     4261 gatctgcatt tatcgcattg ttagcgtaca acgatggaga aaaaagatat ataatagcac
     4321 ctaatacttt gaaagtaggt gatgtagtta actcaggaaa aggtgttgct ccaaatactg
     4381 gtaatgcact ttacctgtct gaaattccac tcggttcatt tatttacgct atcgaaatga
     4441 cgcctggcaa aggtgcatct atagcaagaa gtgccggtac caacggacag ttgatgggta
     4501 gagaaggtaa atatgcagta gtcaaaatgc cttccggtga ggtaagaaga attttgctta
     4561 cttgtaaggc cactatgggt gctacttcca acagcgacca cgcattgacg gtaatgggca
     4621 aggccggtaa aaaccgacat gcaggaagac gaccacgtgt gagaggtgta gctatgaacc
     4681 cagttgatca cccaatgggt ggtggtgaag gtagagcttc gggaggtcac ccgcgttcac
     4741 gtactggtat tatggctaaa ggtcaaaaaa ccagaaatac gaagaagcct tcgagcaagc
     4801 ttattatctc aagaagaaaa acatcaggta gaaaataatt aaaattaaac taaatggcac
     4861 gatcactgaa aaaaggccct tacgttttcc acaaattgct tgataaagta aaagcagcaa
     4921 aagaaaacaa caaaaaagct gtaatcaaaa cctggtcaag atcatctatg attataccgg
     4981 ttatggttgg tgaaaccatc gcagtacaca atggcaaaac atttgtaccg gtttatgtta
     5041 cagaaaatat ggttggacac aagcttggcg aattttcccc aactcgtttg ttcagaggcc
     5101 atggtggtaa gaaaaaataa aagctttaat tgctataata ataagtataa tgaaagcagt
     5161 agcaaaattg aaaaattgtc cgatgtcgcc acgaaaaatg cgacttgtag ttgacaatat
     5221 tcgcggaaaa aaggtagaag atgcgttgaa catcttaaaa tttacaaaca aggaagcatc
     5281 agcatggcta accaaagtat tggtgtcagc agtagctaac tgggaagtaa aaaacggcgg
     5341 aagcgttagt gctgatgact acgctctaat cgtaaaaact gcctttgttg acggaggtac
     5401 aatgctgaaa agatttcagc ctgcacctca aggacgcgct cacagaattc gtaaacgaac
     5461 caaccacata accattgaag tggagaatag aatcccgact ccaatggaca gtgagatggc
     5521 agctgaaagt gcagaataat ttataatttt tgactttaaa aatagaaaca atacatgggt
     5581 cagaaggcaa atccaatagg taacaggtta ggtatcatcc gaggatggga gtcaaactgg
     5641 tacggtggta gaaatttcgc tgataacgtg gtggaagatg aaaaaatcag aatttacctc
     5701 aatgctcgta ttccaaaagg cggaatagca catattatca tcgaacgtac tcttaaaaga
     5761 ataactgtta caattcatac atcaagaccg ggcatcgtaa tcgggaaagg tggcaatgaa
     5821 gtagatcgta ttaaggaaga gcttaaaaag cttaccaaga aagatgtgca gattaatatt
     5881 gtcgaaatca gaaaacctga acttgacgca acaatagtag gcgataccat cgcaaaacag
     5941 cttgagtctc gtatcaatta cagacgagca attaagatgg ccatccagtc aactatccgt
     6001 gccggagcag aaggtattaa ggtgagaatt tccggaaggt tgaatggagc tgaaatggca
     6061 aggtcagaag aatataaaga aggacgtaca ccattacata ctttcagagc tgacatcgat
     6121 tatgctataa gtgaagcttt aactgtttat ggtaaaattg gtatcaaaac ctggatctgc
     6181 aagggcgaag ttttaggaaa gcgtgacttg tcgttcacta cctatgaaga gaaaaaggat
     6241 accaaggatc aaagaaaaag accgggtggt gaaggtagag aaagaggtgg acaacaaaga
     6301 ccaccaaaaa gaaagtagaa tatttgaaaa aaaagacgta attttgcgtg gatttttcaa
     6361 ctaagaatgt tagaaacgta taattcattg atataaaagc atttagcgat gttacaacca
     6421 aaaagaacaa aatacagaaa gcagcaaaaa ggtcgtattg cggatattac atatcgtggc
     6481 gcaatcgtat cttttggatc ttttggttta aaatctgttc agagtggccg tattaccagc
     6541 agacaaattg agtcggcaag gattgcgatg acaagatata tgaaaagaga gggacaggta
     6601 tggatccgca ttttccctga taaaccaata acgtctaaac ccgcagaagt gcgtatgggt
     6661 aagggaaaag gagcactgga tcattatgtt gcaaatgttg aggccggaag aattatgttt
     6721 gagatcgatg gagtaccggc tgatgttgca gttgaggctc tgagactggc tgcacaaaaa
     6781 ttacctttat taactaaaac agttacccga cctgatttgg gctaaaatat caaacatcat
     6841 ggcaactaat aaatatcaag aattaataaa cttttcagat gctgacttgg ctactgagct
     6901 gcaagtcaca gagagagaat ataaaagaat gcaatttgat caggctttga aaggattgga
     6961 aagcccgatt aaacttcgtg aagtcagacg cgacattgct cgcatcaaaa cagaaataag
     7021 aagacgagaa cttagtggta tgagcgaagc agcattggca tcaagaaata aaattgttaa
     7081 cagaagaaga aaatcttaat tagtaaatta ccagacatca tggttgaaag aaagattaga
     7141 aaacagaaaa caggtgtggt ttcaagtaat aaaatggata aatccattac agtacttgta
     7201 ctccgaagat tgcgtcaccc taagtatgga aaatttgtta agaaaactaa caagtttatg
     7261 gcacacgatg aaaacaacga gtgcttgata ggtgataaag ttagaattat ggaaaccaga
     7321 ccgttaagca agaataagag atggcgtttg gtagaagtta ttgaaaaggt taaataataa
     7381 aagtaatatt ttaaacgatg atacaacagg agtccagatt aaaggttgct gataatagtg
     7441 gtgcaaagga agtactttgc atcagggttc ttggcggcac gaaaagaagg tatgcttcaa
     7501 tcggagacaa aatagtagtc tctatcaaag aagccgtacc aggtggtgta aaaaaaggga
     7561 cagtaagcaa agctgtgatt gtccgcacca gaaaggaaat ccgccgggat gatggttctt
     7621 acatcagatt tgatgataat gctgtcgtat tgctgaataa ttcagatgaa ccacgtggct
     7681 cgcgtatctt tggccctgtc gccagagaat tgcgtgacag aaactacatg cgtattgttt
     7741 cacttgctcc tgaagtatta taattataga aaataagtat catggctact aaacaaactc
     7801 aaaaaaggtt tgcacctaag ctcaagatta aaaaagacga taaggtgatt gtcttgtcag
     7861 gtgatgacaa gggaaaacgc ggtaaggttc tgagtgtaga tcctactaaa aaccgcgcaa
     7921 tcgttgaagg tgtcaatatt gttaagaaac ataccaaacc tggtaataac aagccgggtg
     7981 gtattaacga aattgaagca ccggtaaatt tgtcaaattt gtcactaatt gatccaaaat
     8041 caggtgaggc aacccgtgtc ggcagaaaac ttgtcgatgg taaaatggtt agatattcta
     8101 aaaaatcagg cgaaattatt taatacgatg agttacgaat cacttcataa aaagaaatat
     8161 agagacgaaa ttgtaagcca gttgatggaa cagtttcagt acaaaactgt catgcaagta
     8221 cctaagcttg tgaaggtaac tatcaatcaa ggattgggag gtgccacatc tgacaagaaa
     8281 ctggtagaga atgcacttca agaaattgct ctgattaccg gacagaaacc tgttccgact
     8341 aaagcaaaga attcaatctc taacttcaaa ttgagagaag gaatggtaat aggtgcaaag
     8401 gtgacattga gaaacgaacg tatgtgggag tttattgacc gtttattgac cattgcacta
     8461 ccacgtattc gtgactttag aggtattaac aataaatctt ttgacggtcg cggaaattac
     8521 tcccttggta tcactgagca aatcatcttt ccggaaatcg acatcgataa agtgaataaa
     8581 atcaccggta tggatatcaa cttcgtgact acagctaaaa cggatgccga agcttttgca
     8641 ctgcttaagt tacttggttt cccatttaaa aatcaaaaga actaatccaa gtatggcaaa
     8701 gaaatcaata atagcaagag aagttaaaag aaagaagctc gtagaaaagt atgctgaatt
     8761 gcgtaagcag ctaaaagaag ctaatgatta tgatgcattg gacaagttgc cacgaaattc
     8821 taatcctatc agaatgcata accgttgcag attgaccgga agaccaaagg gatttatgcg
     8881 tgattttgga ctctgtagaa atatgttcag aaaaatggca ctcgatggta aaattcctgg
     8941 tatcactaag agtacttggt aataaatttt tagaatagaa tctttgaaaa gaaatataaa
     9001 atggcagtaa cagatccaat agcagattat ctgacaagaa tcagaaacgc tcagatggca
     9061 aaccataaaa tcgtagagat accttcttca cgtatgaaga gaagtattac tgagattttg
     9121 tacgaacagg gttatattga gaaatataaa ttcgacacta cagatcctat tcaaccgatt
     9181 ataaagatcg ccctaaagta taatcctgat actaaattgc cggtaatcag agagttaggg
     9241 agaatcagcc gacctggttt gcgaaaattt agcggttcta aggatatacc gcgtattatc
     9301 aatggtctcg gtctgtgtat agtttcaaca tcaaagggag ttattacaga taaggaagcc
     9361 agaaaactta atgtcggtgg cgagttagtt tgttacgtat attaatattt taaaaataag
     9421 ataggatgtc tcgtatagga aaagcaataa taagattacc agagaaagtt gaggtttcat
     9481 ggactaaagg taatctggta actgttaaag gcccaaaagg tcagcttaat cagcaaattg
     9541 accctgatct atctattaat cttgaagacg gagttctgtc attaagcaga cctactgacc
     9601 agaagagaca cagatcggtt cacggtcttt acagagcact actcaataat atggtgattg
     9661 gtgtttctga aggttatgaa agacaaatgg aactcaacgg ggtaggttat agggtaacta
     9721 ataccaacca aatgcttgaa ttagctttgg gatactctca cccaatccat tttgcgctcc
     9781 cggctgaagt tgctctagaa actttgacag aaaagggttc acctcctaaa attattctta
     9841 aaagtatcga taaacaattg atcggtcagg tggcagctaa aatcagagcc atgagaaaac
     9901 ctgaaccata caaaggtaaa ggtatcaagt ttgtaggtga aatcatcaga cgaaaggctg
     9961 gaaagacttc tggtaaaaaa taataaattt tcaatagctt aagcaatgaa aagaactaaa
    10021 gaagaaaaaa gaagaagaat tcattacaga attcgaaaaa aaataagcgg tactgccgaa
    10081 acaccaaggc tatctgttta taagagtaat actgcaatct attgtcagct tatcgatgat
    10141 gttaatggtc atactttggc tgcagcttct tcaaaggagg caaatgctag tagtggcact
    10201 aagagtgagc aggcaaaagc agtaggagct ctcattgcag aaaaggctaa atctgcgaat
    10261 atacttagtg tagcatttga cagaggtggt tatttgtatc atggtcgcat caaagctttg
    10321 gctgatggtg cacgtgaagg tggtttacaa ttttaataag agaaaagaga aatattaaat
    10381 ggcaaagaaa gtagttcaaa acgttaatcc tgcagaatca gaactaaagg atagaatggt
    10441 tactctcaac cgtgtcgcga aagtaactaa aggtggtcgt acttttagtt ttgcggcaat
    10501 cgttgtcgta ggtgatggta atggtattgt gggtcaaggc cttggaaaag caagagaagt
    10561 aaccgatgca attcaaaagg caattgacga cgcaaagaaa aaccttgtaa aagtacctgt
    10621 gtataaaggt actattccgc atgagcaaaa tggtaagttt ggcgctggta gagttttgat
    10681 taaaccttca gctcatggta ctggtgttat tgctggtgga gcaatgcgtg ctgtactcga
    10741 aatggctggc atcaatgacg ttttggccaa atctctcgga tcttctaatc cacataatgt
    10801 tgtaaaggct actattgatg ctttgtcaaa gttgagaagc ccaattgatg tttcaatgca
    10861 acgttcaatt aaactaacaa aagtgttcga aggttaatct cttcggatga tacattttaa
    10921 ataatagcgt tatgactaaa ttaaagctaa ctcaggttaa aagtgtgatc gacagaccaa
    10981 aagatcaaaa gtttacaatg agatctttgg gcctaaaaaa agtttaccat tctgtagaaa
    11041 ttgatgcaac accatcaaat gtaggtatgg tagaaaaagt aaagcacttg ttgaaagttg
    11101 aaaaagtata aattttagaa ttggttcaaa ttaagtaaga tgaaacttca tacattaagg
    11161 ccggcaaaag gcgcagtaaa aaaatcaaag agaattgcac gcggacaagg ttccggccat
    11221 ggaggcactg ctacccgagg gcacaaaggg gcaggatcac gttctggtaa ttctatcaaa
    11281 cgaggttttg agggtggaca gatgccgctt cagagacgaa ttcctaaaag aggtttcaaa
    11341 aatatcaacc gcattgatta tgttgttgtg aatttaagca ctttacaaaa cctttctgat
    11401 aaatataacg tatcatcctt cgatatcgac ttttttgtaa accatggcat agccaaaagt
    11461 tcagagaaag tcaaagtcct ggctaatggt acattgaaat caaagattag tgttgtagca
    11521 aatgctgcat ctgaaaaagc aaaacaaacc atagaacagc tgggaggaag cattaactta
    11581 gtataattta ttagaatgaa aagcttaatc acgactctca aaaacatttt tagtatcaaa
    11641 gaactcaaag atagaattct atatactttg ttgttactct tgatatttcg tattggaaca
    11701 gaaatcgttc ttcctggtgt aagagcagat gttctcaaat cagcaacaaa tgcaaatgca
    11761 aatgacttgc ttggtttgtt aaatattttt acaggcggag ccttcaataa tgcttctatt
    11821 cttgcattag gtatcatgcc gtatatcact gcctccattg ttgtacaatt attgggattt
    11881 gcagtgcctt atttccaacg tttacagtca aaagaaggtg aaagtggccg taagaaactc
    11941 aatcagataa cgcgtttact tacagtagcc atcacgcttg tacaaggtgg tgcgtatctt
    12001 acttatgtca atagccttgg agcagttgat actaaaatac ctaccggaat cttctggtta
    12061 tgtaactcca taatcttatc aacaggtaca gtattttcga tgtggctcgg agaaagaata
    12121 accgacaaag gtataggtaa cggtatatca ctattgattc aaatgggtat cattgccaga
    12181 ttgcctcagg ccttcgcatt cgaattctct tctcaggctt ccaataacaa tcttctaatt
    12241 ttcatccttg aacttgcctt gttgttcgct gtaataatgc ttacaatact tgtagtacaa
    12301 ggtgttagaa agatacccgt tacatttgcc aatagaatgg taggccgtac ttcaaagtat
    12361 atgccgggtg aaggagccag agactatatt ccacttaaat taaatgcagc gggcgtaatg
    12421 ccaatcatct ttgcccaggc tttgatgttc ttacctgcta ctgttgcaca gtttgcaaca
    12481 aaagatcagt cagcacttgc atctagcagc atttttagtg ctttgaatga ctttatgtca
    12541 ataccttata atattatcta ttttgtaatg gttgttttgt ttacttatat ctatactgcg
    12601 cttattgtta attcacagca atacacagat taccttaaga gacaaaatgc ttttattcct
    12661 gggattaaac caggttcgca caccaacgaa tttatagaca gtattacaag taggattaca
    12721 ttaccgggtg cgatattcct tggtattatt gcaatctttc cggcaatagc tggacgagtg
    12781 ttgggtatta accattcatt tgcagtattt tttggtggaa catcactatt gattctagtt
    12841 ggagttgctc ttgatacact tcaaatgatt gaaagttatc ttttgatgag aaaatatgat
    12901 ggactggtcg agtccggcaa aatacagggt agaagcagct ctggtattaa tataggtgct
    12961 gggtattaag ttcaaaacca atattgattg aatgatcttt tataagacag acgaggaaat
    13021 tgaactgatt cgtcagagta atttgattgt ttctggtgca cttgcagcag tagcagcaga
    13081 gctccgacca ggcattacag gtcttaaaat cgatcagata gctgaaacat atattaggga
    13141 tcagaaagct gaacccggat tcaaaggtta taatggtttt cctgcaagtt tgtgcatttc
    13201 ggtgaataat caggttgtac atggtatccc atcaaatatc ccatttaagg aaggtgacat
    13261 agtttctgtt gactgtggcg ttcaaaagaa tggatttttt ggagattccg cgtatagctt
    13321 tggactggga atcttatcac atgagcataa aagattgctt gaagtgacat tggagagttt
    13381 ataccttggc atcgaaaaag ctacagttgg taatagaatt ggtgacattg gttatgctat
    13441 ccaaagctat gttgagaata atggttattc tgtcgtccga gatttgatag gtcacggagt
    13501 tggaagagat ttgcatgaac ctcctgaagt acctaatttc ggtcggcctg gtaaaggaac
    13561 catgttacag gaaggactag tgattgccat tgaacctatg attaaccttg gtaagaaaga
    13621 agttagggtt ggagaagatg gttggacggt cattacaaaa gatttactac catctgcaca
    13681 cttcgaacat tccattgccg taaggaaagg tggcgttgat atattgtccg attattcaat
    13741 tattgaagag gaaataaaaa agaataaaaa tattgagcaa ttatcaatta aaattccgat
    13801 atttgcacct cgaaattaac atgaatggca aaaaaggatc ttattcaaca agatggtatc
    13861 attgaagaag cattatctaa tgcaatgttc agggtcaggt tggaaaataa ccacacgatc
    13921 ttggctacca tttcaggaaa aatgagaatg aattatattc gtattctacc tggcgataaa
    13981 gtggcaatcg aaatgtcacc ttatgattta tcaagaggac gaataattta cagatataaa
    14041 taaagaaaat ttttaaaata cgtagaaaat gaaagtacgt gcttcaatta aagtaagaac
    14101 agctgattgc aaaatcgtaa aacgaaacgg caagttgtat attattaaca aaaagaatcc
    14161 taagttcaag caacgtcaag gttaataaaa gaataatatc aaatagtaat atattatggc
    14221 gagaattgca ggtatagatt tgccgaaaaa taaaagaggt atcataggtc ttacttatat
    14281 ctatggcatt ggtccaaaca tttcacgaga tattttgtct caagcagaaa tagatgaaag
    14341 tgtcaaagtt gctgactgga atgatgagca aataaagaaa attacaagta tcatccaaaa
    14401 ccaatacaag gtagaaggtg aattgcgttc tgaaattcaa cttaacatta agagattaat
    14461 ggacatcgca tgttaccgag gtcttcgtca ccgtaaaggg cttcctttac gtggacaaag
    14521 cacacagact aacgctagaa ctcgtaaagg taagcgtaag actgttgcca ataaaaagaa
    14581 agttactaaa taataattaa gatttaaaag catatactga atatggctaa agcggtcaaa
    14641 aaagttaaaa aacgcaaagt aagaattgac tcagacgggt tagtttttgt aaaggcatct
    14701 ttcaataatc tgattgtctc tctttgtaac agacaaggtc aggttatttc atggtcatca
    14761 gccggaaagg ttggcttcag aggttcaaag aaaaatactc cttttgcagc tcaaaaagct
    14821 gccggtgaag cttctctaac tgcttacgaa gccggaatgc gtactgccaa cgtctatgta
    14881 aaaggacctg gcgccggtag agaggctgct ataagagctg ttgatcaggc gggcattaaa
    14941 gtattacgca tttttgatgt taccccggta ccgcacaatg gttgcagacc acctaagaga
    15001 agaagagtat aattattttt aattattttt ataatggcaa gatacacagg acctaaaact
    15061 aaaaaatcaa gatcttttgg agaagctatc ttcggattcg ataaagcttt tgaaaaaaag
    15121 aaatatccgc cgggagtgca cggacttaaa cgcaagaaaa agcaaaagtc tgactactct
    15181 atgcagttga ctgaaaaaca aaaagctaaa tatacttacg gactcttgga aagacagttt
    15241 cgtaatctat ttgtaaaggc tgctgctaaa tcaggtgtaa cgggtgaaat cctccttcag
    15301 ttccttgagg gtcgccttga caatgttgtt taccgcctcg gtatttctcc ttcacgacct
    15361 ggagcacggc agctagtaac acataagcat attcttgtta atggcaaact agctaatatt
    15421 ccatcaatgt tgcttcaacc aggtgacgtc atttctgtgc gaggaaaatc gcaaggacat
    15481 gattttataa ccaacaatgt gcgtggaaaa aatgaagtga agaaatatgc atggctagag
    15541 tggaatccag ataaaatggc tggaacctat atacaggctc cttcacgcga tcagataccc
    15601 gaaaatatca acgagcagct tattgttgaa ttatattcta agtaatttta ttttatctgc
    15661 ctggtcggtt ttcgcccggg cagataaatt tctgtattta atatttttat tttcaaatcc
    15721 ccctcaaaca gaattctttt atgagtattc taaattttca aaagcctgat aaaatcttat
    15781 tacaaaaagc aacagatttt gaaggagttt ttgagtttaa acctttagaa ccgggttttg
    15841 gtcaaacagt aggtaattca ttgagaagag tattgctttc ttccctagaa ggtgtcgcaa
    15901 tctcggctgt tagaattgcc ggtgttgatc atgagttctc taccattaaa ggtgtggtcg
    15961 aagatacagt agaaattata ttgaacctta aacaagtaaa tcttaaacaa gtattaagag
    16021 acgaagatat tacatcagaa aagatttatg ttacactcag tggaagaact gagttccgtg
    16081 caggtgatct tgaagacttt acaaatgttt ttaaagttat gaatccggat ttgcttattt
    16141 gcagaatgga accttcagtg acacttgaaa tggagtttac cataacaaaa ggacgcggat
    16201 atgtaccggc tgatgaaaat cttccaaaag atgcaagcat aggtgttatg cctattgatg
    16261 ctatctatac gcctatcaaa aatgtagcat ataaagtatc aaatacaagg gttggacaac
    16321 gaacggacta tgaaaaattg accattgaaa ttacaactga tggcactatt caccctgaag
    16381 atgctataaa agacgcttca aggattttga tccaacactt gatgttgata acagacgaaa
    16441 atattacctt cgaagatgct actagccgcg aagataatat agttgatgag cacatactgc
    16501 acatgcgcaa attgcttaag acttctttgg aggatcttga tttgtctgta cgtgcctaca
    16561 attgtctgaa agctgcaaaa attaatacac ttggcgaact tgtacgtttc gatatgaatg
    16621 agctgcttaa gtttagaaac ttcggcaaaa aatcactagt tgaaatcgat gagttacttc
    16681 aagaaaaagg tcttactttt ggtatggatc tttcaaaata cagactcgac gaagattaat
    16741 atatttcttt aacattttgc aataactgtt atgagtgtga gctccggtgt tgcgaagata
    16801 aaaacattcg aaaatgagac acggtaacaa agtcaaccac ctcggtagaa aaaccggcca
    16861 cagagcttca atgctcaaga acctttcaaa ctctttgatc tctgcaaaga gaattaatac
    16921 tactttggca aaggcaaaag tgttgagaac tcaccttgag ccattgatca ctaaagcagt
    16981 aaaagaaaat actacccatt ccaggagaac tgttttttca tacctccagg ataaagaatc
    17041 tataaaggag ctttttggcg ttattgctga taaagttggt gatagaccag gcggatacct
    17101 tagaattatt aaaacaggaa cacgtatagg tgacggtgcc cctactgctc tgattgaatt
    17161 tgttgacttc aatgattctt accagataac tactaagagt gacgctaaga aaaaacgtac
    17221 tcgtagaagt ggtggtgcta aaaaatcaac cgaaactgca actgccgtac agcaaactac
    17281 cccggtagta acagatacag caacagaagc tgtagaggat gtagtaacta ctgcggaaga
    17341 aattgtagat aatgctaccg aatcgacagg tgatgttaca aacacagata gcaatgaaaa
    17401 taaggatgca taattaagcc ttgcttgatt agcagaatac aaacggcgat gaggtttttc
    17461 ttcatcgccg ttttattttt tgctgaagga tttttttttc atggactgaa acagttatct
    17521 ttgccaaaat tatacacatg caagcaggtt cggatagaaa agctatcttg ttattggaag
    17581 acggaactgt ttttgaaggc cgggcagccg gcaaaatcgg tacagccact ggagaaattg
    17641 ctttcagtac agggatgacc ggatatcagg aagtatttac tgatccttct ttctacggac
    17701 aattgcttgt cgcgaccaat gtccacatcg gcaattacgg gatccgctat cctgaatccg
    17761 aatcaggtag tatcaagata aatggtttca tctgtcgtaa ttttactcca agctattcgc
    17821 gtcaaatggc agatgactct attcaggaat attttgaaaa ggagaatttg gttgccatct
    17881 ccgggattga tacacgttct ctcgtcaaac atatcagaga aaagggtgct atgaacggta
    17941 ttatttcatc cgaaatcctt gatgtcgcac agcttaaatc aactcttgca attgtgcctt
    18001 caatggatgg gctagaactg gcttcaattg tctcaactaa agagccttac gattatggca
    18061 gtccggaggc aaaatataga gttgctgtac tcgattttgg catcaaagaa aacattctca
    18121 gatgtttggc acaaagggat tgcttcatgc gggtttttcc ggcaaaaact aattttgctg
    18181 aacttgaaca atggaacccg gatggctatt tcttgtcaaa tggaccgggt gaccctgctg
    18241 caatggatta tgcactcgaa acagttcggg aaattgtaaa aaccaacaaa aaagtatttg
    18301 gcatctgtct tgggcaccaa cttattggcc tgagtgtagg cattaacact tataaattac
    18361 accatggtca ccgcggtatc aaccatcctg taaaaaactt gatcactgga agatgcgaaa
    18421 taaccagcca gaaccacggc tttggtttgg atgcaaaaaa tattgagcaa ttcaaggatg
    18481 tagtggaaat ttcacatgtc aatcttaatg ataacaccat cgaagggatt agattgaaga
    18541 atggcaatgg attttctgta caatatcatc cggaggctgc tcccgggcca catgatgcaa
    18601 gatacctgtt tgatgatttt atcgaattat taaataaaaa ttaaccagat gagtgcaatc
    18661 attgatatta atgcaagaga aatcttagat tcacgaggta accctaccat cgaggtcgat
    18721 gtcattacgg aaggtggagg ttatggtaga gcagcggttc catctggggc atctaccggc
    18781 aaacatgaag ctgtagagct acgagatggc gatactaaac gttttggagg aaaaggtgtg
    18841 ctaaatgcag tccaatttgt tgaagatgtc atttcacctg agctcattgg cctagatgtc
    18901 ttcgaacaaa gagaaataga ccaaaccatg atacagctcg atggtacacc caataaatca
    18961 aaactgggag caaatgctat acttggtgta agtcttgcag cggccaaagc ggcatcatca
    19021 gaaagtgata tgcctttgta tagatacata ggtggggtca attcgtttac acttcctgta
    19081 ccaatgatga atatattgaa tggtgggtct catgccgata ataaaattga ttttcaggaa
    19141 tttatgatca tgccggtcgg tgctgataca ttcagagagg gcctcagaat gggtgttgaa
    19201 atatttcatt cacttaagaa ggtgctgtca gccaaatctt attcaacaaa tgttggtgac
    19261 gaaggcggtt ttgcgcccaa tatccagtca aatgaagaat caattgaact cgtattgcag
    19321 gctatcgaaa aagcaggata caagcccggt gacgaagtat tgatagcaat ggatgccgcc
    19381 gcaagcgaat tttacgatga aaataaaaaa atatacgatt tccacaaatc tggaagtaag
    19441 ccattgacag gtgaagaaat ggttgatttt tgggtaaaat gggccagcaa atatccgatc
    19501 ttctccattg aagacggtct gcatgaagat gactgggact cttgggcaat gctcacccaa
    19561 aaacttggct caaagattca actggtaggt gatgatttgt tcgttacaaa ttcggaaagg
    19621 ctccgcagag gaattgaaaa taatattgcc aactcggtac ttgtgaaggt aaatcagata
    19681 ggaaccctaa gcgaaaccat agatacagta acaatggcac acagaaatag atatacaact
    19741 gtcatcagtc acagaagcgg agaaacagaa gatactacca tcgcagatct tgcagtagca
    19801 ttgaatgccg gacaaattaa aacgggttct gcctctcgct ctgatcgtgt tgccaaatac
    19861 aaccaacttt taaggataga agaagaattg ggtacaagtg cgtattatcc tggtaaaaat
    19921 ttactaaaac ggttgtaact ttaccctaaa ttacctggca aatggaaaaa ataaaagaaa
    19981 ttacagacaa aatacctaaa tggctcttca acaaattcgc aataaccacg gtattgttta
    20041 ttttttggat ggtgttttta gacaaataca atctctttac ccagtttggt atgatgagtg
    20101 acattcgtaa gctaaaacag gaaaaactga tgtatgccga gaaaatagaa aagcttaagg
    20161 ccgaaaaaga agcgttcgaa cacaaccttg agaaatacgc acgcgagaaa tacctgatgc
    20221 acaagtcaaa tgaagatgtt tatatagttt tagataaaag taaataaaaa gcatgtcaat
    20281 tttagttaat aagaattcaa aagtaatcgt tcagggtttt acaggtaatg aaggcacctt
    20341 tcatgccgaa caaatgatag cctacggtac gaatgtagta ggtggcgttg tacctggtaa
    20401 gggtggccag cagcatctgg gcagaccagt atttaatact gttgcagatg cagtccgaga
    20461 agcaggggca gatgtgtcca tcatctttgt tccaccggct tttgcagcag atgctattat
    20521 ggaagctgcc cagtcaggga ttaaagtaat tgtatgtatt acagaaggta ttccggttca
    20581 ggatatgctc aaagcaaaag attatatcca aagattcgat tgcagactga tagggccgaa
    20641 ctgtccgggt atcattacgg cagatgaagc aaaaatcggt atcatgcctg gcttcgtttt
    20701 caaaaaaggt aaaatcggta tcgtttcaaa atctggtacg cttacctatg aagcagcaga
    20761 tcaggttgtt aaggccggcc ttggtatttc tactgctatt ggtattggcg gcgacccgat
    20821 tatcggtact accacacgag aagcagtcga aatgtttatg aatgaccctg aaaccaaagg
    20881 catcgttatg attggtgaaa tcggaggatc actggaagca gaagctgcca actggataaa
    20941 agcaaatggc aacaagaaac cggtagtcgg gttcatagcc ggacagacgg ctcctaaagg
    21001 gagaaaaatg gggcatgcag gagctatcat cggtggggca gacgatacag ctgaagcaaa
    21061 aatgaaaata atgagcgaat gcggtattca tgtagtaagt tcaccagccg aaattggtga
    21121 cacaatgcga aaattggtct gaatctgaat tcagctaaag ataataagcc accggatttt
    21181 tttcctggtg gcttttttct tgctcgcaac gcatcgtact tcttaatatt tattccttta
    21241 taggtaggcg aacaaagaat gtagaacctt tatcgggtat agtagagaag taaatttcac
    21301 catccataga ttctatcaac ttcttgcata tagcaagccc aaggcccgag ccacttgatt
    21361 ttgtagtaaa attaggcata aaaatcttct ccttcttcga atcatcaatt ccgtgcccat
    21421 tgtcttttac ggctataatg atcttgttcc cttcccgtcg tgtcaaaacc tctactcgcc
    21481 caatacgttc gctcggtatg gcctgaacag cattttttat aagattgttg atgattcgta
    21541 tgagatggct tttgtcggca tgcacgatta tatcttcatc caatattgat tttttaaaat
    21601 tcagatgctc attacctgtt ttctcgaata attcgtaaat accattaact atcgtattga
    21661 ggtgcaaatc ctcgctgtta atttttggct cctgtgcaaa ttcaccgaag gtgcccgcaa
    21721 tagccgacag gttttcaatc tgctgtacga gtgtgtcaca aatattcttg atacgctgtt
    21781 ctcgtgattc cggatctgag tggtagctgt gtagcagata ttgcacattg agtttcatag
    21841 gtgtcaatgg attctttatt tcatgtgcta cctgtctcgc catttcacgc catgcttctt
    21901 cacgttctga tctagcaagc aatcctgcac tatgttcgag tttgactatc atttggttgt
    21961 attctcctac gagaaaaccg atttcgtcat tttttgacca agagataggt tcattttgct
    22021 tacccagttt aaaacgattc aatttgtcac ctaatacaag caatggcttg gtgattgaac
    22081 gccctacagc cagtgccaaa gcagaagcaa taagtagcag aaacacataa gtattgatga
    22141 gtgcgcctat gaagtccgct atctggttat tcgtcgagag gtctgtatgc gaaaatggca
    22201 tgacaatata tccgttaaca gttgaatcgg gagctaaaac ggtatgtacc aggtacgaac
    22261 cattcaatcg attgaaaaag cctcctatct gttgctgaga aaaggtgttc attaccatag
    22321 ggtgctttac aagatattgt agctcaggtt gtaccgtttc tattttggcc ggaaatgaac
    22381 ctgctatgta tcccttttta tcataaataa taatctccga ctccaactct tcgttgaatg
    22441 caaccaaatc tgtttcgtcg ggtttcatgt ttttgtcaat aagccggttt gagatgtctg
    22501 ttacctttct aatcagattt tctgataaat tctttttgtt gctgaaattg aaataaataa
    22561 ttgtaatgat acctattgct acaaaagaaa ccacaatcat tgacacaatt gccacctgaa
    22621 tcctgtttcg gagtgaaatg tttgattggt tttcaaatgt gtatattcct ggaatgaatt
    22681 ttacaaaggt atttaatgca atgaaaaaca agaaagtcac tgccagcagt acaaaaatat
    22741 acgagaatag gctcagtatt ttgatgagcc caccgcctgt cttattgatt tccaacactc
    22801 gccctgttgg gtcttctgct cgcccaccaa gcacttgaaa ccgctggttt attaggttct
    22861 tgtaataagc cgctgtgttt tgaatgtcgt tgttcctgaa gtttggactt tggctggtga
    22921 gatgtccctg atagtataat gcatacccat atttttcaat at
//
LOCUS       DAOQQY010000016        22046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_704_length_22046_cov_5.708699, whole genome shotgun sequence.
ACCESSION   DAOQQY010000016 DAOQQY010000000
VERSION     DAOQQY010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 22046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22046
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_704_length_22046_cov_5.708699"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PLE23_01885"
     CDS             <1..1158
                     /locus_tag="PLE23_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMX82045.1"
                     /translation="MAGPTPVSALIHAATMVTAGIYMITRLNGLFLAAPFTLDVILVV
                     GTATALVAAIIATRQNDIKKVLAYSTVSQLGFMFAALGAGAFVSGMFHLTTHAFFKAL
                     LFLGSGSVIHAMSGEQDIRNMGGLKSLKVTHLTFLIGTLAIAGIPPLSGFFSKDEILV
                     NVYSKSFWFFIILFATAMLTAYYMFRLYFLTFHGKFRGTKEQADHLHESPLSMTFPLM
                     VLAILAVVGGFLGIPEVFHMPHTFNEMLGKIVKIPELTVSHTFEYTVMGISTLIVGAI
                     IYLTYQYFVTKEKLPSKSEKGFWSTAISNKFYIDEAYDSLVVAPVMGLSKWLYRVLDR
                     SLTDGLVNQVGLTTLLSGQSIRKLQTGRTSLYLFAMVLGLILILCLNFFNR"
     gene            1155..2612
                     /locus_tag="PLE23_01890"
     CDS             1155..2612
                     /locus_tag="PLE23_01890"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMX82046.1"
                     /translation="MISLSLLLLILPLVFSVITGFLSGKNARASAMAGALINLVVAGY
                     AFTQFEANTSYQFAFLSDWIPSIRAKFHIGIDGINLLLILLTNLLLPVIIYTVKHEDS
                     NRSGLYYGLILFMQFALLGVFMALDALLYYIFWELALIPIYLILLGFSSQPDFKTFVK
                     FFIYTLAGSLFMFIAILYVYIKAPGGNFSIDNFYNNHLSEQEQVWVFLGFFIAYAIKI
                     PIIPFHTWQPDTYTKASTPGTMLLSGVMLKMGTFSLLRWLLPVAPLALATVTPVVIIL
                     CIIGIVYGSIIAIRQKNIKTLFAYSSLAHVGLISAGVFALNADGFNGALIQMFNHGII
                     IVGLFLIAEIIYTRTETYHISELGGIRNEAPTLAFTFLIIILASVALPLTNGFVGEFL
                     LLNGVFQYNQWMAVVGGTTVILSATYMLRMYQRTMLGLDRDYTKTILDLGTVEKIMVL
                     GLVLIIIVTGIWPSILLDPIKPAVENLLNIIKIVQ"
     gene            2612..3985
                     /locus_tag="PLE23_01895"
     CDS             2612..3985
                     /locus_tag="PLE23_01895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HMX82047.1"
                     /translation="MTALIILSLLGILVLMMGLFHLKNKVLILIIPVLIAGIFLNANE
                     YGLNASYFSQMVHFDNFAISFSSLLILTTTLIFGLCLQQYKTSKKPVEDIYALIIFSL
                     VGAICMVSFNNLIMFILGLEIMSVPLYVLAGSNRENVLSNEASLKYFLMGAFASAIVI
                     FGMTLIFGATNSFQLDEIRNAVSGTKMPGVLQVGILLFIGGSLFKAAAVPFHFWAPDV
                     YQGSPTVITAFMATVVKTAAFAGLFRFMMVAFQDISGLWVNILVIITIATLFLANFSA
                     LSQTDFKRLMAYSGISHAGYMLIAILVLNMNAADALFFYSVSYSLATIVAFGIFLTMK
                     NATGEYSLEIFKGLSRKNPFLAFGLVIAFLSLAGIPPLAGFFGKYFIFTSAIKGGFLT
                     VVIIAIINTMIGAYYYLKVLNMAFTKDVYHSAPVKNHLLLDAVIVFAAIGTLILGFFP
                     GFLQGFI"
     gene            complement(4507..6699)
                     /locus_tag="PLE23_01900"
     CDS             complement(4507..6699)
                     /locus_tag="PLE23_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX82048.1"
                     /translation="MKNPFYKLLILLLLPVLVNAQEKQDTMHTIKLKAIEITGQLNKN
                     TTVTKLPEVYNTYIVSGKKNEVIKLENANVNLAEKTGRQVFAKVPGVFVYDMDGSGNQ
                     INVSTRGLDPHRSWEFNIRQDGIMVNSDMYGYPASHYAMPLEAISRIELIRGTSALQY
                     GAQFGGMINYINKSPDTSRNVGFENVSTVGSYGLMSSYNSIGGKIGKLTYSGYFAARK
                     SDGYRKNSDSKYDGEYLSLNYDASKTLAIKAQLARSNYVYHIPGPLNDSMFAADPRAS
                     TRSRNYFNPEIWVPSLQFFFNPSSRTSIELTSSAVLGERRSVQIEGAAGTWDLIDPAT
                     GNYKPRQVDIDGFNSYTSELRLRHDYSLLGQNSTLIAGVRYINNDLKRRQVGVGTTGS
                     DADFSADPNSFKRDLSFKTNNIALYVENLFKLTHKWSISPGFRYENGVSKMSGIIKYL
                     DAADVPNEIKHNFALLGISTQYEFNANTKAYAGFSQAYRPVIFKDIIPSSILETTSKN
                     LKDAYGYNLEAGVAGKLGSKLTYDLSVFSVYIKNRLGAQVITDDTSKVTIFRTNIGDS
                     RTNGIEVFVQYNIFETSTTSMNIFTSTSYRDSKYENAVVRAGNENKNVDGNQVQSIPN
                     WISRNGIEVQYKRIGFNVLHSYVSESFADPLNTVTPPKNGSVGLVPEYHLIDAGFSYI
                     VSKNFQLRLNVNNLLNKSYFTKRPEFYPGPAIWPSDGRSVQFSVSTKF"
     gene            7009..7824
                     /locus_tag="PLE23_01905"
     CDS             7009..7824
                     /locus_tag="PLE23_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMX82049.1"
                     /translation="MAENNQTPATPKKESNLFAYLVIPVSLLVCTLIYVFVFGDANNF
                     INNDIHNNPKEGNYLGIIYKGGPIVPVLMTLFLIVLVYTIERMLTISRAKGNGNVDDF
                     LKTVKASIERNDINSALAACDKQRGTVANVVKATVLKYQQMEKEEKMTKDQKVIAIQK
                     EVEESTSLELPMLEKNLVFLATIASVATLVGLLGTVIGMIKAFAALAIAGNPDATALA
                     TGISEALINTALGILTSSIAIIMYNVFTTRIDALTYRIDEAGVSIAQTFASKH"
     gene            7919..8551
                     /locus_tag="PLE23_01910"
     CDS             7919..8551
                     /locus_tag="PLE23_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMX82050.1"
                     /translation="MPKVKIPRKSTLIDMTAMCDVSFLLLTFFMLTTQFKPDEPVVVD
                     TPASISETKLMDDGVITLTMTKEGKVFFSMPGQNTRTAILEDMSQRYKVPFTDEQIKT
                     FAKMESFGVPVAQLPALLDLTPEQRKTFDQPGIPMDTSKNELVGWVEASMIAYSAEKD
                     EKPKIAIKGDGKAEYPSVKKLIDALQAKNFNTFYFITDLEANPNKKKGEE"
     gene            8587..9150
                     /locus_tag="PLE23_01915"
     CDS             8587..9150
                     /locus_tag="PLE23_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMX82051.1"
                     /translation="MAQMDDSGGGSKHKGGGVRSKKMSTRVDMTPMVDLAFLLVTFFM
                     LTTTFNKPTVMDIKMPAKTKDENQNQEIKESESMTVIMDGGDTLWYYHGITDPVLEMS
                     NYTDNGIRQIILKKKEELKSKSDTARINVLLKATARASYKNVVDMFDEMSITSVPVYA
                     LVDMSKVDKDLIFAKTGRMPAEAPPAK"
     gene            9188..10003
                     /locus_tag="PLE23_01920"
     CDS             9188..10003
                     /locus_tag="PLE23_01920"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMX82052.1"
                     /translation="MEPNVDFKKLDILDIIFEGRNKEYGAYLLRKKYPVHIKKALIAG
                     VLIFIFFMTLPFWLKYLKPAQDDTKKEKVEIQLKNLEAPPEKEEEKPPPPPPPPKKVE
                     PPKPPQQQFTPPVIKKDEEVKKEPPKIEEITTNTGTETVKGEDKGYVPQIEEPPAAPA
                     AVEAEPEKVFNFVEQSPTFPGGDAALMDYLRKNIKYPPIARENNVEGRVLISFVVDKN
                     GKIRDITVKRGIGSGCDEEAVRVVKSMPEWKPGRQNGKAVNVMYNLPISFKLQ"
     gene            10008..10292
                     /locus_tag="PLE23_01925"
     CDS             10008..10292
                     /locus_tag="PLE23_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82053.1"
                     /translation="MLKFKYRSDISTSLVSDFFSFMRKEHNNHLASKVVSYFTSLMAL
                     IYCIGGLYLLTSESMKEQLGINFCIAIGIGLIAYGVFRTYRAFQYWKYGE"
     gene            10411..11358
                     /locus_tag="PLE23_01930"
     CDS             10411..11358
                     /locus_tag="PLE23_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HMX82054.1"
                     /translation="MKSIFIPIVLTAILALAACQSSTTTDGNQLSTPTSGQITIAVDE
                     TLKPVIDAQLSVFAHDYPKADITPKFVPEGEAINMLLNDSVQLAIVCRELTDKEKAFL
                     KTRDYKAIQTKSAKDGIALIVNPANTDTLLKYDKMMSILKGEITDWKQLNPKSKAGKI
                     QLVFDQALSSTYRYITESTGVLNLETKNFYAVGNDEKVIEYVSKNKEAIGFIGSSWIS
                     DKNDSTALSFIQSVNVLGISPKPGADGQNEYFQPFQAYIAQDAYPFTRTIYAVCSEPR
                     AGLATGFSSFMAGPKGQRIFLKSSVVPAKMPIRLIRVTK"
     gene            11401..12975
                     /locus_tag="PLE23_01935"
     CDS             11401..12975
                     /locus_tag="PLE23_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82055.1"
                     /translation="MKNLFFVVFCITMSFTVQGQISASVQKAIYYQDFSSAKMNLQKD
                     IKSNPNNLDNYFELGALYARLNKVDSAGIIFGKLAEMNPKAAVTTVANGYVLLNSGKA
                     DEAKAMFEKAAKSTKFKDGSIMRRIGEAYLYSPKTNLDEAIEYLKKAVAIEIKNPVTY
                     YSLGEAYYLKKEGGPAVTNYEFCTDYDKSAAWAYTRIGQIFEGAKNYVKANEAYQAAL
                     SADPDYPFIYKEMANWLYLRRDYKAAMNNFIKYQQLTGDNSIEAKTQKSTYAFYNKDY
                     KQTLELVNDIMKVDSSKNYMIRLLGYSSFEQGDSLSAKNYMEKFFQKTNKDKIIWSDY
                     YYLGKINEKFNNDTEALVNFKKAIELDSNQVDVYDAIAKMYFANENYKEAGYWYGLKL
                     NKLDNPKIQEMFDPAFAYYRSEDWANANTFFGKLVTRYPTSVAGQLYYARTNSLLDPK
                     ATNGLAKPIYEKVAELGEKDPVKYKNELKEAYNYLYYFYYNGNDKTKAFEFADKFTKV
                     DPNDPDGAKLLEAVKQ"
     gene            13131..13943
                     /locus_tag="PLE23_01940"
     CDS             13131..13943
                     /locus_tag="PLE23_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82056.1"
                     /translation="MEYSNRRPFEQNRRPYEQNRRPQDNHRNPNTFAILELKYGNIYG
                     GPPNYFVISDVSLLVFDPEKNHIFLESLSLNSDVDVVFVNSKVNELGHTIGRHKHVVN
                     LSTRKKKPYLENFEVEKNDLENAFANSRPSKKMINNFFFKNFKKYNINKIITFDGRRD
                     IFLCEKAGVKFNYIKIEDLQQDLNRECNYLFSLNKLGVAINFRNDASFLYSNNLKYYL
                     HPIAARQIEPKSAAFDAARLLMVYNEFTQHKENFLLKAALLLNKIQQNTVPE"
     gene            14062..14135
                     /locus_tag="PLE23_01945"
     tRNA            14062..14135
                     /locus_tag="PLE23_01945"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14096..14098,aa:His,seq:gtg)
     gene            complement(14318..14818)
                     /locus_tag="PLE23_01950"
     CDS             complement(14318..14818)
                     /locus_tag="PLE23_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82057.1"
                     /translation="MRDIAILWVLTIAFIQNLDAQHYSLATGIRIGDNYGITVQGKLF
                     RKTSAEVILHPGVGNNSHSVSGLIKRHQKILTDRISLFAGGGIIQQWKNTDTDVTGNL
                     NTGGIQTILGAELTIGRYNISWDFAPSLFLWGSKSGALAGGTAVSVRYVLIAPPSKKF
                     KLKLRK"
     gene            15025..15843
                     /locus_tag="PLE23_01955"
     CDS             15025..15843
                     /locus_tag="PLE23_01955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMX82058.1"
                     /translation="MKNKIHQILSYITYFIRAKTIYGIDSPVLYAFFNSVLDDKREYY
                     AFRRINQLRSALKTNFTRIKRTDFGTGKNEHTTTVSNIYNTSASSKLKGELLFRLVKW
                     FKPKEIIELGTNLGISTAYLASVHTKTMVNTIEADPLLSQIAEENLHDLQLTSVRIIN
                     GTFENELTRLLSLHPDTNFFFIDGNHAFDPTLAYTSAILCQQQTNFLIIYDDIYWSTE
                     MTKAWNELKNNKEFNFIIDIYHFGIVGHIPNLRKPIRKTLISTKKKIWQMGFFR"
     gene            complement(15844..18477)
                     /locus_tag="PLE23_01960"
     CDS             complement(15844..18477)
                     /locus_tag="PLE23_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX82059.1"
                     /translation="MSDISHNLPSTKNYRFRELKVYASTEWLADNRKKYRQVFDRFDT
                     SYIYTELSFYNKLFDEDDWEIELELKCFVLKKEKKEICNLAFKRKVSKFDNVVYIREG
                     WGNKKPGAFWKKGTYFWEVWVKNEKIATKYFYIEEAENRFLEEINPYVEIQSLKLYEG
                     PYDDVPELERVYYKSYSEEDTRYIYSEIVLKNNNFNKAWQLELFIKFFNDARELKGQI
                     VRLMKVEKKEELIRLTAGWGSNVKGSWRKEKYTVEIVFLDKLLAVIPFQVGDTFEEGV
                     SGVMLPANQSTTMLVEVEEDTVPFEEVFDELDKLIGLHDVKRKVKEHAQYLQFLKLRK
                     EKGYKESDEMLVHSVFTGNPGTGKTTVAKMMGKLYKKMGLLSRGHVHEVDRVDLVGEY
                     IGQTAPKVREAIDKARGGVLFIDEAYALARSNDDSKDFGREVIEILVKEMSNGDGDLA
                     VIVAGYPKEMKYFIDSNPGLKSRFKLYFDFTDYLPQELSVIADYAAREKHIKLSDAAK
                     AKIDEKIVNAFRTRDKTFGNARFVFDLIEQAKMNLGLRIMEAKHPEQMPTEELAIVLP
                     QDIDKISTTTNRILPNIPVDAGLLNSSLAELDSLIGMPEIKARIHELVRLVQFYKETG
                     KNALNTFSLHTVFLGNPGTGKTTVARILTSIYKALGILERGHMIETDRQGLVAGYVGQ
                     TAIKTTEKLDDSLGGVLFIDEAYTLTMKGGSAHGDFGDEAVQTILKRMEDDRGLFFVF
                     VAGYTENMENFLKSNPGLSSRFDQILKFEDYSPDELLEIALVMIKNENYHITDDAKDL
                     LHEKIKNLHLYRDKYFGNARTVRQIIQELMKFQNLRMAGYSSEEYKNIDTNLINVDDI
                     NSLTVFGVKDIFEKKGIGFNK"
     gene            18793..>22046
                     /locus_tag="PLE23_01965"
     CDS             18793..>22046
                     /locus_tag="PLE23_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82060.1"
                     /translation="MTTIDAYSGLGDMDDFLSPQSSSGCLGGEINSVWLIIQIDESSA
                     PGNTLTMLITPKNGLAADYDWAIYGPNAPCGILGMPVRCSAAPGNCFYCPGTGIAGGQ
                     GFPLNSEDKASGDGLLPLLSTKPGDVYYICVNNYDDDPGGFSIQFGGTAILDCDPNPP
                     CVMQVSVSPDVTVCQGEAGTFQMHGSEIGGGGLVDWLWTADPAIAVNFLDNPNIADPS
                     VTIPPSFSGPITYTLTVTNPACFTSKKVKVYVTPTPLVTLDPIPQDICSSADPIQLNF
                     SPPGGYFDAFGPIDNGFLDPSQWGGGIFNIKYTVPFGNGCVGDNEITINILNAPLAQI
                     DQIDDFCVTDPVYQVLAYDTGGKWSGNGVNAKGEIDPKKLGPGTFSAIYTTDNGVCKT
                     KDEVLFKVNPLPNVKITDPGPICNDTILITLKGSPAKGKWSGLPDSLGKFNPNITKPG
                     TYKVFYSYTDLFGCKNKDSLNIKINPAPFAVITPSAQVCNSNQNGNTSLLDFSLLISA
                     GDAGGSWQDIDGSGATGTFPVLDFLNAAPGKYNFTYTTNSALPGCDEVSYTIEVEVVD
                     CKCPALFLTPSTILCNDLANYDLSNLKLNTNDGTWSIINTPPGSNPALITGNNINIQN
                     KDTGNYTIEFKLVTTPPPGCDNTNTTEITLHAPPVLTFAPTKTVCNKILPGSSPVTLD
                     LDTYITSPDKSGIWTEITSSGASGTMPFLDFTGVTPGTYTFSYTTNSALAPCTETTKT
                     VDVIVQDCECPSLALTKQLELCNDGTTYDLNLQKITTEPGSWTLKQTPPGVNPISLTG
                     SIISISNKDAGNYVFSFTLANNPPAGCANSDDITVSLFDSPEATVLSTAEVCNSNSSG
                     NTTILNFNDFIISGSKTGVWLDLNGSGASGSFNSKDFTGVMPGNYTFSYTTNTANFPC
                     TDKTYNIIITVNDCNCPGLGLIKSTSICNEGNTQIDLNNLKITSESGNWAVKSKPTGN
                     PANLTGSLFDVSNAATGNYVITFTLQNTPPPGCPLSADLTIEVLAPPVATLNNTVSVC
                     NSTGSGQYSTVLNLYNQITAGDKSGSFEDTDNSGAKPIGNQLDFTGVAPGNYSFT"
BASE COUNT         6772 a         4312 c         4128 g         6834 t
ORIGIN      
        1 atggcaggtc ctacacctgt atctgccctc atccacgcag ccacaatggt gacagccggt
       61 atatacatga ttacaaggct gaatggtttg ttcctcgctg caccatttac attagatgtt
      121 attctcgtag taggcacggc gacagctctg gtggctgcca ttattgccac tcgccagaat
      181 gatataaaga aggtgctagc ctattctacc gtaagccagt taggctttat gtttgctgca
      241 cttggcgcgg gtgcatttgt ttcaggaatg tttcacctta ctacgcatgc cttttttaaa
      301 gcattgttgt tccttggatc tggaagcgtc atccacgcaa tgagcggtga acaagatatt
      361 agaaacatgg gaggtttgaa atctctcaaa gttactcacc ttactttctt aattggtaca
      421 ttggctattg cgggcattcc gccattatca ggtttctttt caaaagatga aatattggta
      481 aatgtttatt cgaaatcttt ttggttcttt attattttgt tcgctaccgc aatgttaact
      541 gcgtactaca tgttcagatt atatttcctt acctttcacg gaaaattcag gggaacaaaa
      601 gagcaggccg accatcttca cgaatcgcca ctttctatga catttccatt gatggtattg
      661 gctattctag cagtggtagg tggttttttg ggtattcctg aagtattcca catgccacat
      721 acattcaacg aaatgcttgg aaaaattgta aagatacctg aacttacagt gtcgcatact
      781 ttcgaatata cagtaatggg tatttcaaca ttaatagtcg gtgcaatcat ttatttaaca
      841 taccaatatt tcgttacaaa agaaaaactt ccttcaaaat ctgaaaaagg attctggtca
      901 acagccattt ccaataaatt ctacattgat gaagcatacg atagcttggt agtagctccc
      961 gtaatgggtt tgtcaaaatg gttgtaccgt gtattggacc gttcacttac tgacggactt
     1021 gttaaccaag tgggtttgac aacattactt tccgggcaaa gcatacgcaa gctacagaca
     1081 ggacgtacaa gtttatattt gtttgcaatg gttttaggat tgattcttat actttgttta
     1141 aattttttca atagatgata tcattatccc tactattatt aatcttgcct ctggtatttt
     1201 ctgttattac aggattcctt tcaggtaaaa atgcaagagc ctcggctatg gcaggagcac
     1261 tgattaatct ggttgtggcc ggctatgcgt ttactcagtt tgaggccaac acatcttatc
     1321 agtttgcttt cctttcagac tggatacctt ccataagagc taagttccat ataggcatag
     1381 atggtatcaa cttgttattg atattgctta ctaacctatt gcttccggtt atcatctaca
     1441 ccgttaaaca cgaagacagc aaccgttccg gactttatta cggcctcata ctatttatgc
     1501 agtttgcgct gcttggcgta tttatggcat tagatgcact gttgtactat atattttggg
     1561 agctagccct tataccgata tatcttatat tacttggttt ttcttcgcag cccgatttca
     1621 agacatttgt caaattcttt atttatacac ttgcaggatc actatttatg ttcattgcca
     1681 tattgtatgt gtatataaag gcacctggcg gcaatttttc aattgataat ttttacaaca
     1741 atcatttatc tgagcaagag caagtctggg ttttcttagg attcttcata gcttacgcca
     1801 ttaaaatacc gatcattcct ttccatacat ggcagcctga tacttatacc aaagcatcaa
     1861 caccaggcac catgttattg tcaggtgtaa tgcttaaaat gggtactttc agcttactca
     1921 gatggttatt gcctgttgcg ccgcttgcat tagctacagt aacaccggta gtaataattc
     1981 tgtgtatcat tggtatcgtt tacggttcaa tcattgccat caggcaaaag aatatcaaaa
     2041 ccttatttgc atattcttcg ctggcacacg taggccttat cagcgctggt gtatttgcac
     2101 ttaacgcaga cggattcaat ggtgcattga tacagatgtt taatcacggt ataattatcg
     2161 ttggtctgtt tttgatagca gagattattt acaccagaac agaaacctat catattagtg
     2221 aacttggagg aataagaaac gaagccccta ccctggcatt caccttcctg atcattatcc
     2281 tggcttctgt agccttacca ttgaccaatg gattcgtcgg agagtttctt ttgctcaacg
     2341 gagtattcca gtacaatcaa tggatggcag tggttggcgg aactaccgtc atactgagtg
     2401 ctacttacat gcttagaatg tatcaacgta caatgcttgg gctcgaccgc gactatacta
     2461 agacaatact agatcttggt actgttgaaa aaattatggt attgggctta gtgctcatta
     2521 taatagtgac cggaatatgg ccttcgatat tacttgaccc gattaagccg gcagtagaaa
     2581 atttattaaa tatcattaaa atagttcaat aatgacagct ctcattattt tgtcattgct
     2641 cggtatcttg gtgttgatga tgggtctgtt ccatcttaag aacaaggtat taatccttat
     2701 tattccggtg ctaatagccg gtatttttct caacgccaat gaatatgggc tgaatgcaag
     2761 ctatttttca caaatggtgc attttgataa ttttgccatt tcgttttcat cattattgat
     2821 attgaccacc acgcttattt ttggcttgtg tcttcaacag tataaaacca gtaaaaaacc
     2881 ggtagaagac atctatgcgc tgatcatctt ttcgctcgta ggagctatat gtatggtttc
     2941 gtttaataac ctgattatgt ttatactcgg gcttgaaata atgtctgttc cgttgtatgt
     3001 actggccggt agcaaccgcg aaaatgtact gtcaaacgaa gcatcattga aatatttcct
     3061 catgggagct tttgcgagtg ccattgtaat tttcggaatg acactcatct tcggagctac
     3121 caattcgttt caacttgatg aaataaggaa tgctgtttct ggcacgaaaa tgcctggtgt
     3181 attgcaggta ggtattctgc tgtttatagg tggttcgttg tttaaggcag cagccgttcc
     3241 tttccacttt tgggctccgg atgtatatca gggttctccg actgtcatta ctgcatttat
     3301 ggctactgtt gtaaaaaccg cagcttttgc cggcttattc agattcatga tggtagcatt
     3361 tcaggatatt tcaggtcttt gggtgaatat cttggtcatc ataaccattg ctactttgtt
     3421 ccttgccaat ttctcggcac tcagccaaac tgatttcaaa aggttgatgg cctactcagg
     3481 aatttcacat gccggatata tgcttattgc tattttggtg ttgaatatga atgctgcaga
     3541 tgcgcttttc ttctattctg tatcatacag ccttgccacg atagtagctt ttggcatatt
     3601 cttgactatg aaaaatgcta caggtgaata ctctctggaa atattcaaag gactatcgcg
     3661 taagaatcct ttccttgcat ttggcctggt catcgccttc ttatcacttg ccggtattcc
     3721 accactcgca ggcttttttg gtaaatattt catatttaca tcggctatca aaggtggttt
     3781 tcttactgtc gtgattatag ccattatcaa tacaatgatt ggagcttatt attatctaaa
     3841 agtgttgaat atggcattta ctaaggatgt ttatcattct gctccggtta aaaatcacct
     3901 cttgctggat gcggttatag tttttgctgc aatcggtact ttgatacttg gattttttcc
     3961 gggatttttg caaggtttta tttaacccag atatcgcatt aggttcttta ctgtaaagaa
     4021 tgaatacttc aaatccattt taaaagttca gaagctatag ttcacttcgc ttttaatact
     4081 tttgcatgga ttatttgtac taatcatgtt cgtttatatt ctctaatggc ttttctacgt
     4141 aaacatagtt ctcgactaat aaagagattt gcgtgaagga tggtaggcgg cacaacgtaa
     4201 gccggacagc ctgacccgac aggggcacgc cctgattaaa cgcggataac acattagatt
     4261 ctttgcagca aataagatta cttccaattg gtttcaaaag cttaaaaacc atggttcagt
     4321 ttgcttttat tacttttcca tgaattgatt gtactcatca ttttcgttta aatttctagt
     4381 gcgttatccg agttttatac aagggcatta ttacctttca aaatttatct caatgaacat
     4441 tagcaaaaaa aagagcggaa aatatattcc actctttttt gatctactat tattttcaaa
     4501 ttataatcag aactttgtag atacagagaa ttgaacgctt cttccatcag acggccagat
     4561 tgcagggcca ggatagaact cgggtctttt tgtgaaataa ctcttattca aaagattatt
     4621 gacattcaat ctcaactgaa aattcttaga cacaatgtat gaaaaccctg catctataag
     4681 atgatattcg ggtaccagcc caaccgaacc gtttttaggt ggagtgacag tattgagagg
     4741 atcagcgaat gactcagaca catacgaatg taaaacattg aaaccaattc gtttgtactg
     4801 tacctcaatt ccatttctgg atatccagtt aggaatgctc tgtacctgat ttccgtcaac
     4861 atttttattt tcattaccgg ctcttactac agcattttcg tatttgctat cacggtatga
     4921 ggtggaagta aaaatattca tactggtagt gctggtttca aaaatattat attgtacaaa
     4981 tacctcaata ccattcgttc tgctatcacc gatattggtt ctaaaaatgg taactttgct
     5041 tgtatcgtct gtaataacct gggcaccaag tctgtttttg atatacactg aaaatacact
     5101 taaatcatac gttagcttcg aaccaagttt tcctgctaca cccgcctcaa ggttataccc
     5161 ataggcatct ttaagatttt tactcgtggt ttccagtatt gaactcggaa taatatcctt
     5221 gaaaataacc ggacggtaag cctgagagaa tccggcatag gcctttgtgt ttgcattaaa
     5281 ttcatactga gtgctgatgc ctaagagagc aaaattgtgc ttaatttcat tcggcacatc
     5341 agctgcatca aggtatttga taatgccact cattttcgag acaccgtttt catacctgaa
     5401 acccggagaa atgctccatt tatgagttaa tttgaacaag ttttcgacat agagtgctat
     5461 gttattggtt ttaaagctaa gatctctttt gaaagaattt gggtcagcag aaaaatctgc
     5521 atccgaaccg gtagtaccga caccaacctg acgtcttttt aggtcgttat ttatatagcg
     5581 cacacctgca attaaagttg aattttgccc aagcaagctg taatcatgtc tcaatcgcag
     5641 ttcgcttgta taactgttga atccgtcgat atctacttga cgaggtttat aattgccggt
     5701 agcggggtca ataaggtccc aagtgccggc agctccttct atctgtacac ttcgtctttc
     5761 tccgagtaca gccgaacttg tgagttcaat agaagttctt gaagaaggat tgaaaaagaa
     5821 ctgtagtgaa ggaacccaaa tctcagggtt gaaataattt cttgacctgg tagaagctcg
     5881 agggtctgct gcaaacatcg aatcattgag cggacctgga atgtggtaaa catagtttga
     5941 tcttgctaac tgagctttga tagccaaggt cttgcttgca tcgtagttta gactcaaata
     6001 ttctccatca tattttgagt cactgttttt tctatagcca tcagattttc ttgctgcgaa
     6061 atatccggaa taggtcaatt tacctatttt accaccgatt gaattatagc tggacatcag
     6121 gccgtaagag cctacagtcg aaacattttc aaatcctaca tttcgcgaag tatcgggcga
     6181 tttattgata taattaatca taccgccaaa ctgagcacca tactggagtg ccgaagtgcc
     6241 ccggattagt tcaatgcgtg aaatagcttc taggggcatg gcataatggc tggcagggta
     6301 accgtacata tcagagttga ccataatgcc gtcttgtcgt atattaaact cccaactacg
     6361 gtgagggtca agaccacgcg tagaaacgtt tatctggttg ccagagccat ccatgtcata
     6421 aacgaaaacg cccggaacct tagcaaacac ttggcgtccg gttttttcag caagattgac
     6481 attcgcattt tccagcttga ttacttcatt cttttttccg gatactatat aagtattata
     6541 cacttcaggt agcttagtaa ctgtagtatt tttatttagt tggccggtaa tctctatcgc
     6601 tttaagcttg atagtatgca tcgtatcctg tttttcctgt gcattcacca gtactgggag
     6661 aagtaatagg atcagcaatt tgtaaaaggg atttttcata ttaagttatt aattaattaa
     6721 ttaatcatcc gtaattttac tctaaaagta ttagtatgtt tctatttgca aattaaaaac
     6781 tcattaaaat atattaaatt taagtcaata tgataatgtg cataaaatct ttgcaaacta
     6841 ttgcgttaat attattatga aaatttggtt acatagagtt gtgatttaag tttttaccac
     6901 tttaacaaaa atttttattg aaacatgcac aacatacata cgttttaacc tattttcgtg
     6961 actgaatttt aactaataat caatttaaat tttaaaacat ccgcaattat ggcagaaaac
     7021 aaccaaactc cagcaacacc aaaaaaggaa agtaatcttt ttgcttattt ggttattccg
     7081 gtgtctttac tggtgtgtac acttatttat gtctttgttt ttggagacgc taacaacttc
     7141 atcaacaatg acatacacaa caatccgaaa gaaggaaact atcttggtat catttataaa
     7201 ggaggtccaa tcgttcctgt cctgatgacg ttatttctga ttgttttggt ttacaccatt
     7261 gagagaatgc ttaccatttc acgcgcaaaa gggaatggta atgttgatga tttccttaag
     7321 accgtaaaag catctattga gcgcaatgat ataaattctg ctcttgcagc ttgtgacaaa
     7381 caaagaggca cagtagccaa tgtagtaaaa gctacggtac tgaaatacca gcaaatggaa
     7441 aaagaagaaa agatgacaaa ggatcagaaa gtcatagcaa tccaaaaaga agtagaggaa
     7501 tcaacttcac ttgagcttcc tatgctagaa aagaaccttg tgttcttggc tactattgct
     7561 tcagttgcca cattggttgg actgttaggt acggtaattg gtatgatcaa ggcgttcgct
     7621 gctcttgcta ttgccggtaa tccggatgca actgcccttg caacaggtat ctcagaggca
     7681 cttattaaca cggctttggg tatcttgacg tcttctatag ccatcattat gtacaacgtg
     7741 ttcacaactc gtattgacgc tttgacatat aggattgatg aagctggagt gagcattgca
     7801 cagacttttg catcaaagca ttaatttttt tgattcacct aaccaactaa tttgtaaaaa
     7861 aatatgtcat catatataga ggtgcatatt tgatcggtta atcaaaaatt aattaattat
     7921 gccaaaagta aaaatacccc ggaagagtac tttgatagat atgaccgcta tgtgcgacgt
     7981 atctttcttg ttgctcacgt tttttatgct tacgacacag tttaaacctg acgaacctgt
     8041 cgtagttgac acgccggcat ccatttcaga aacaaaactc atggatgatg gtgtcataac
     8101 attaactatg acaaaggaag gtaaagtttt cttcagcatg cccggccaaa atacaaggac
     8161 tgcgatactt gaagatatga gtcagcgata caaagttcct ttcacagatg agcaaataaa
     8221 gacttttgct aaaatggaaa gcttcggtgt accggttgct caattgccag cattacttga
     8281 tctgactccg gagcaaagaa aaacattcga ccaaccgggc attccgatgg atacttccaa
     8341 gaatgagttg gtaggatggg tagaagcttc tatgatagcc tattctgcag aaaaagacga
     8401 aaagcccaaa atagctataa agggtgatgg caaagcagag tatccttctg taaaaaaatt
     8461 gatagatgca ctacaggcca aaaactttaa tacgttctat tttattacag atctcgaagc
     8521 caacccgaac aaaaagaaag gcgaagagtg aaaaattatt gaataatcaa ataaaaagca
     8581 tgatatatgg ctcaaatgga cgactccgga ggaggatcga aacataaagg tggaggcgta
     8641 agatcgaaga aaatgtccac tcgcgtggat atgacaccga tggtggacct ggcatttctt
     8701 cttgtcacgt ttttcatgtt gaccaccacg ttcaacaaac ctaccgttat ggatataaaa
     8761 atgccggcca agactaagga tgaaaatcaa aatcaggaaa taaaggaatc agaatccatg
     8821 acagttatca tggatggcgg tgatacactt tggtattatc acggtattac tgaccctgtc
     8881 ctcgaaatga gcaattatac tgataatggt atccgtcaga tcatcttaaa aaagaaggaa
     8941 gaacttaaat ccaaatcaga tactgctcgg attaatgtac tccttaaagc tacagccaga
     9001 gctagttaca aaaatgtagt ggatatgttt gatgaaatgt caatcacttc agtacctgta
     9061 tatgctcttg ttgatatgtc aaaggttgac aaagacttga tttttgcaaa aacaggaaga
     9121 atgccggctg aggcacctcc tgcgaagtaa taaccacatt tattggctat aattatagaa
     9181 tatttctatg gaaccaaatg tagattttaa aaaactagat attcttgata tcatcttcga
     9241 aggaaggaat aaagagtatg gtgcatatct gttgcggaag aaataccctg tccacatcaa
     9301 aaaagccctt attgccggtg ttctaatatt cattttcttc atgacacttc ctttttggtt
     9361 aaaatacctt aagcctgctc aggatgatac caagaaagag aaagtagaga ttcaattaaa
     9421 gaatctcgaa gcaccacctg aaaaagagga ggaaaaacca cctccaccac caccaccacc
     9481 aaaaaaagtg gaacctccta aaccacctca acaacaattc accccaccgg tgatcaaaaa
     9541 agatgaggag gtaaaaaaag aaccaccgaa aattgaagag atcacaacta ataccggtac
     9601 tgaaacagta aagggtgaag acaaaggcta tgtacctcag atagaagaac cgcctgcagc
     9661 acctgctgct gtagaggccg aaccagaaaa ggtatttaac tttgttgaac agtcacctac
     9721 tttccctggt ggtgatgcag cattgatgga ctatctgaga aaaaatatca aatatccacc
     9781 gattgcaaga gagaacaatg ttgaaggacg ggtactcatt agctttgttg ttgacaaaaa
     9841 tggcaaaatt cgtgatatta ccgtaaaaag aggtatcggg tctggctgtg atgaagaagc
     9901 agtaagggta gtgaaaagta tgccagaatg gaaacccgga agacaaaacg gaaaagcggt
     9961 gaacgttatg tacaacctgc caattagttt taaactccaa taattaaatg ttaaaattca
    10021 aatatcggtc ggatatcagt acttcactgg tatccgattt ttttagtttt atgagaaaag
    10081 aacacaacaa ccatcttgcc agcaaggtgg tcagctactt tacgagtctt atggccttga
    10141 tatattgtat tggcggtcta tatctgctta caagcgaatc aatgaaggaa cagttaggca
    10201 taaatttctg tattgccata gggattggac ttatagctta tggtgtattc agaacataca
    10261 gagctttcca atactggaaa tacggcgaat aagttaattt cgcgttaata acttcaatca
    10321 ttcaactgaa tattggagta atattgttga tttaatgaaa ctaagcccta tttttgtact
    10381 ttgttagagc ataaacagat cagaacgctt atgaaaagca tattcattcc aatagtgcta
    10441 acggctatct tagccttggc agcttgccag tcaagtacta cgacagatgg taatcaatta
    10501 agcacaccta caagtgggca aattacaatt gcagttgatg aaacactcaa acctgttatt
    10561 gatgctcagc tttctgtttt tgctcatgat tacccaaaag cagacataac tccaaagttc
    10621 gtcccagaag gagaagctat taatatgcta cttaatgatt ccgtccagct ggccattgtt
    10681 tgccgtgagc tcactgacaa agaaaaagct ttccttaaaa ccagagatta caaagccata
    10741 caaacgaaaa gtgccaaaga tggtatagcc ttgatagtta acccagctaa tacggatact
    10801 ttactaaagt atgacaaaat gatgtctata ctcaaaggtg aaatcacaga ttggaaacaa
    10861 ctaaatccga aatctaaagc aggcaaaata cagctcgttt ttgatcaggc gctttcatca
    10921 acatacagat atattacaga atcaacagga gtgcttaacc tcgaaacaaa aaatttctac
    10981 gcagtgggta atgatgaaaa agtcatagaa tatgtgtcaa agaataaaga agccatcggg
    11041 ttcatcggtt cgagctggat atctgacaaa aatgacagta cagcactcag cttcatccaa
    11101 agtgttaatg tattaggtat ttcaccaaag cccggagcgg acggacagaa cgaatatttt
    11161 caaccttttc aggcatatat cgctcaggat gcctaccctt ttacccgaac tatttatgcc
    11221 gtatgtagtg agccccgtgc aggtctcgca acaggatttt catcttttat ggccggacct
    11281 aaaggacagc gtattttttt aaaatcgagt gtagtacccg caaaaatgcc catcagacta
    11341 atacgagtga caaaataatt atttttttaa tttgaaatgt ataatccaat caatagtaaa
    11401 atgaagaatt tgttttttgt tgttttttgt atcacaatgt ctttcactgt acaaggtcaa
    11461 atttctgcaa gtgtacagaa ggctatatat tatcaggact tctctagtgc gaagatgaac
    11521 ttgcaaaaag atataaaaag taatccgaat aaccttgata actattttga actcggtgct
    11581 ttgtatgcca gattaaacaa agtagattcg gctggtataa tattcggaaa actggcagaa
    11641 atgaatccaa aagctgcagt aactaccgta gccaacggtt atgtgttact caacagcgga
    11701 aaagctgatg aagctaaggc gatgttcgaa aaagctgcca agagtacaaa gttcaaggac
    11761 ggctctataa tgcgtcgtat tggtgaggct tatctttatt caccaaagac aaatctggat
    11821 gaggcaatcg agtatcttaa gaaagcagtg gccatcgaaa tcaaaaatcc ggttacatat
    11881 tattctcttg gagaagctta ctaccttaaa aaagaaggtg gcccggcagt gaccaattat
    11941 gagttttgta cagattatga caaatcggcc gcatgggctt atacccgtat cgggcagata
    12001 ttcgaaggcg ccaagaatta tgttaaagca aatgaagcat accaagccgc actatctgct
    12061 gaccccgact accctttcat ttataaagaa atggccaact ggctttatct gagacgtgat
    12121 tacaaagctg ctatgaataa ttttatcaag tatcagcagt tgacaggcga taatagcata
    12181 gaggccaaaa cgcaaaaaag tacctacgct ttttacaaca aagattacaa gcaaacactt
    12241 gaactagtca acgatatcat gaaggttgat tcatcaaaaa actatatgat tcgactgttg
    12301 ggttattcca gttttgaaca gggtgacagc ctttcagcga aaaactatat ggagaaattt
    12361 tttcagaaga ccaacaagga taaaattatc tggtcagact attattacct tggcaaaatc
    12421 aatgaaaaat ttaacaatga taccgaagca ctcgtaaatt ttaaaaaagc catagaactc
    12481 gactctaatc aggtagatgt gtatgacgct attgctaaaa tgtattttgc aaacgaaaat
    12541 tacaaagaag ccggttattg gtatgggcta aaactcaaca aacttgataa tccaaagatt
    12601 caagaaatgt ttgatccggc ttttgcttac tacagatcgg aagattgggc aaatgcaaat
    12661 accttttttg gaaaattggt aacaagatat cctacaagtg tagcaggtca actatattat
    12721 gcacgcacca actctttact agaccctaag gccaccaacg gacttgcaaa accaatctat
    12781 gaaaaagtgg cagaattagg cgaaaaagac cctgttaaat acaaaaatga actaaaagag
    12841 gcctataact atctgtacta cttctactac aatggtaatg ataagaccaa agcatttgag
    12901 ttcgctgata aatttaccaa agtagatcct aacgacccgg acggagcgaa attgctggaa
    12961 gcagtgaaac aataagcttt aaagaataag tgcaatattc tgatgatttt ttaacaaaat
    13021 attgcactta ttgaaacaaa aaccttctga gaagtgtata tttgatagta ttgtactaaa
    13081 atctcaacaa aacaaataaa ctgattcgat aaccttgaaa tttacctggc gtggaatact
    13141 ctaaccgaag accatttgaa caaaacagaa ggccgtatga acaaaataga aggcctcagg
    13201 ataatcacag aaatccgaat acttttgcta tacttgagtt aaagtatggt aacatatatg
    13261 gtggtcctcc aaattatttt gtcataagtg atgtctcttt attggtcttt gacccggaaa
    13321 agaatcatat attccttgaa agcctttctc taaactcgga tgtagatgta gtgtttgtca
    13381 atagcaaggt caacgaactt gggcatacta ttgggagaca taaacatgtc gtaaacctga
    13441 gcactaggaa gaaaaagcca tatctggaaa attttgaagt agaaaaaaat gatcttgaaa
    13501 atgcatttgc caattcaaga ccttcaaaaa aaatgatcaa taattttttc tttaaaaatt
    13561 ttaagaaata taatatcaat aaaatcatca cttttgatgg aagacgtgat atattcttgt
    13621 gtgagaaagc aggtgtaaaa tttaattata taaaaattga agatttgcag caagatctga
    13681 atagagaatg caactacttg ttttcgctta acaaacttgg tgtcgctatt aatttccgaa
    13741 atgatgccag cttcctgtat tcaaataacc ttaagtatta tcttcaccca atcgctgcca
    13801 gacaaataga accaaaatct gccgcctttg atgctgcaag attactaatg gtgtataatg
    13861 aatttacaca gcacaaggaa aattttctac taaaagctgc tctgttgctt aataaaatac
    13921 aacagaacac agtaccagaa tagataaaga atacataggg ggattgctat ttcgtatcgc
    13981 cgaaaattat tttaatattt tttcgtagaa tgctttttac tgataatgta aatccctata
    14041 tttgcacttc attttgaaat ggtgattata gctcagctgg ttagagcgtc ggattgtggt
    14101 tccgaaggtc gccggttcga acccggttag tcacccttat gtaatgcata ttacaataca
    14161 ataaggaaaa ttccctggat gcttatgcca ttccggggat ttttcttttt tagcccaaat
    14221 agtgcattaa gaatttaaaa cgaaaattac ggcgttttta gcctttttga aaaaatagca
    14281 tccaatgcat ttatttttca aagctgtttg ttttggatta tttgcgtagt ttaagtttaa
    14341 attttttcga aggtggagca atgagcacat acctgacaga cacagccgta ccaccggcca
    14401 aagcaccact tttgcttccc caaagaaaca gcgaaggtgc aaaatcccag gatatattgt
    14461 atctgccaat tgtaagctcg gcaccaagta tcgtttgaat gcctcctgta tttaaatttc
    14521 ctgtaacatc tgtatctgta tttttccatt gctgtattat tcctcctcca gcgaatagac
    14581 ttatccgatc tgtgaggatt ttttggtgtc gtttaataag gccggatacc gagtgactgt
    14641 tattgcctac acccggatgt aaaatgactt ctgcagatgt ttttctaaat aattttccct
    14701 gaacagtaat accatagttg tccccaatac gtatccctgt ggccaagcta taatgctgtg
    14761 catcaagatt ttggatgaag gcaatagtta atacccataa aatagcaata tctctcataa
    14821 tcgataaacg tttgacttta catcatatta tgttgaacca aataatcaat ctattattga
    14881 acgttcaact atccggtaaa gttggctttg gcataaaatt ctcaatggtg gatagaaaat
    14941 tgcacagcta ttgctaattt tacagataat atagcatttt tcaattcaag aacaaaattc
    15001 caattaaagc ccaaccatta gtttttgaaa aataaaatac atcaaatact cagttatata
    15061 acatacttca ttcgtgctaa gacgatatat ggtatagatt cacctgtctt gtacgcattt
    15121 ttcaattccg tccttgatga caaaagagaa tattacgctt ttaggcgaat aaaccaattg
    15181 cgttcagcat taaagaccaa ttttacaaga ataaaaagaa cagactttgg aacaggaaaa
    15241 aatgaacata ctactactgt atcaaatatt tacaacactt cagcttcatc aaagctaaaa
    15301 ggagaattat tgttcagatt ggtaaaatgg ttcaaaccta aggaaatcat agaacttggc
    15361 acaaatctgg gcatttcaac agcatatctt gcctcagtac atacaaaaac aatggtcaat
    15421 accatagagg ccgacccact gttatctcaa atagcagagg aaaatctaca tgatctccaa
    15481 ctaacaagtg tccgcatcat aaatggcact tttgaaaatg aactaactcg attattaagc
    15541 ttacatcctg ataccaattt cttttttatt gacggcaatc atgcatttga cccaacatta
    15601 gcttacacaa gtgcaatact ttgtcagcag cagactaatt ttctcataat atacgacgat
    15661 atatattggt caacagagat gactaaagcc tggaacgaat taaaaaacaa taaagaattc
    15721 aattttatta ttgacattta ccattttggc atagtcgggc atatacctaa tttgcgcaag
    15781 ccaataagga aaacacttat ttctacaaag aaaaaaatct ggcaaatggg tttttttagg
    15841 taattattta ttaaaaccga tgcctttttt ctcaaaaata tcctttacac caaatactgt
    15901 cagtgaattg atatcatcca cattaataag attcgtgtca atattcttgt attcttctga
    15961 agaataacca gccatccgca gattctggaa tttcatgagt tcttgtataa tttgccttac
    16021 cgtacgggca tttccgaaat atttatcacg ataaaggtga aggtttttta ttttctcatg
    16081 aagcaaatct tttgcatcat ccgtaatatg gtaattttca ttttttatca ttacaagagc
    16141 tatttcaagc aattcatcag gtgaataatc ttcaaactta aggatttgat cgaatctgct
    16201 gctcagcccc ggatttgatt ttaagaaatt ctccatgttt tctgtataac ctgctacaaa
    16261 gacaaagaaa aggccacggt catcctccat ccttttgaga atggtttgaa cggcttcatc
    16321 accaaagtcg ccgtgggcag aaccgccctt catggttagc gtataagctt catctataaa
    16381 caatacacca ccgagtgaat catccagttt ttctgttgtt ttgatagctg tttgccctac
    16441 gtagccagct acgagtccct gacgatcggt ttcgatcatg tggccacgtt cgagtatgcc
    16501 aagggctttg tatattgaag tgagaatcct ggctactgta gttttaccgg ttccgggatt
    16561 gcccagaaaa acagtatgta gcgaaaatgt attcagtgca ttttttccgg tttctttata
    16621 aaactgaacc agacgtacaa gttcgtggat tcttgccttg atctcaggca taccaatgag
    16681 cgaatcaagt tcggcaagtg acgaattcaa taacccggcg tcaaccggaa tattaggcag
    16741 tattctgttg gtagttgtac ttattttatc aatatcctga ggtagtacta ttgctagttc
    16801 ttcagtaggc atttgttcag ggtgttttgc ctccataatg cgcaggccga ggttcatctt
    16861 ggcttgttct attaggtcaa atacgaatct tgcattgcca aaagtcttgt cacgtgtgcg
    16921 gaaagcgttg acgatttttt catcaatctt ggctttggcg gcatcgctaa gcttaatatg
    16981 tttctcgcga gcggcataat cggcaatgac tgataactcc tgtggtaggt agtcagtaaa
    17041 gtcaaaataa agtttaaacc ttgatttcaa acctgggttc gaatcaataa aatacttcat
    17101 ctcctttgga tagccggcaa cgatgacggc cagatcacca tcaccgtttg acatttcttt
    17161 aacgagtatc tcaataacct cgcgtccaaa atctttgcta tcgtcattgg atcgtgccaa
    17221 agcataggct tcatctatga acaatacacc tcctctggct ttatcgattg cttcccgaac
    17281 ttttggggct gtctgtccta tgtattcgcc tacgaggtca actctgtcca cttcatgcac
    17341 atgtccacga ctcaaaagac ccattttctt ataaagtttc cccatcatct tggctacagt
    17401 tgtcttacct gttcccggat tcccggtaaa gacagaatgt acaagcattt catcactttc
    17461 cttgtatcct ttttctttac gcaatttcaa aaactgaaga tattgtgcgt gttctttcac
    17521 ctttcgttta acatcatgca aaccgataag tttgtcaagt tcgtcaaaca cttcttcaaa
    17581 aggtactgta tcctcttcta cttcaacaag cattgtggtg gactggttag ctggcaacat
    17641 aacccctgac acaccttctt caaaagtatc accaacctga aacggtatga cggccagcaa
    17701 tttatctaga aaaactattt caaccgtata tttttccttt cgccatgagc cttttacatt
    17761 agaaccccac cctgctgtaa gccttatcaa ttcttccttt ttctccacct tcatcaatcg
    17821 cactatctgc cctttgagtt cacgagcgtc gttaaaaaac tttatgaaca attcaagttg
    17881 ccaggcttta ttgaagttgt tgttttttag gacaatttct gagtaaatat atctggtatc
    17941 ttcttcagaa tatgatttat aatataccct ttcaagctca ggtacatcat cgtaggggcc
    18001 ttcatatagt ttaaggctct gaatttcgac atacgggttt atctcttcaa gaaatctgtt
    18061 ttcagcttct tcaatgtaaa aatatttggt ggcgattttt tcatttttta cccagacttc
    18121 ccagaagtat gtgccttttt tccagaaagc tcctggtttt ttattgcccc aaccttcacg
    18181 tatatatact acattatcaa atttacttac ttttcttttg aatgcaaggt tacaaatttc
    18241 cttcttttct ttttttagta caaagcactt caattcaagt tcaatctccc aatcatcttc
    18301 atcaaataat ttgttgtaga atgaaagttc tgtgtaaata tatgatgtgt caaatcgatc
    18361 aaatacctgt cgatacttct tgcgattgtc agcaagccac tcagtagagg catatacttt
    18421 aagctctctg aacctataat ttttcgttga tggcaaattg tgagaaatat cagacattga
    18481 cgattgtttt tattgaataa taacgcattt aataattgat cagtttctgc aaaactcaaa
    18541 gatggtgtta ttttttagaa ttttttttta ataaattgtt aaagatggaa caacaaaaac
    18601 tgaactataa gtatctatat ttgtgactgt aagaattttt aaattaattt aagtaaaata
    18661 caaatagctg gttacgtgaa aagtctaatg ttaaaagtgt gtgttgttgt gttgactatc
    18721 ataacaattc ctggttattt gtgctcacag aattccgttc aggattgttc tggtgcaatt
    18781 ccagtttgtg gtttgaccac cattgatgct tattctggcc ttggagatat ggacgatttc
    18841 ctttctccac aatcatcatc aggttgtctt ggaggtgaaa taaattcagt ttggttaatc
    18901 atccaaattg acgaaagttc tgctccgggc aataccctaa cgatgcttat tacacctaaa
    18961 aacggtttgg ctgctgatta tgactgggct atttatggtc cgaatgcacc ttgtggtatt
    19021 cttggtatgc ctgtaagatg ttctgcggca ccgggtaatt gtttttattg tccaggcact
    19081 ggtattgcag gtggacaagg ttttccttta aattcagaag acaaggcaag tggcgacggt
    19141 ttacttccac ttttgagtac aaaacctggt gatgtatatt atatatgtgt caacaattat
    19201 gatgatgacc ccggaggatt ttcaatacaa tttggaggca ctgcaatact tgattgtgac
    19261 cctaacccac cttgcgttat gcaggtttct gtttctcctg atgttactgt atgtcagggc
    19321 gaagcgggca cttttcagat gcatggctca gagataggtg gcggcggact cgttgactgg
    19381 ctgtggacag cagatcctgc aattgccgtc aatttccttg acaatccgaa tattgcagat
    19441 ccgagtgtaa ctataccacc gagctttagt gggcctataa cttatactct cacagttacc
    19501 aatccggctt gttttacttc caagaaagta aaagtgtatg tcactcccac tccacttgtt
    19561 acattagacc ctataccgca ggatatttgt agttcggcag atccaataca gctcaatttt
    19621 tctccacccg gtggttattt tgatgcgttt ggcccgatag acaatggttt tcttgatcca
    19681 tcgcaatggg gcggtggcat tttcaatatt aaatatactg taccttttgg aaacggctgt
    19741 gtgggtgata atgaaataac catcaatatc ctgaatgcac cattggctca aattgaccaa
    19801 attgatgatt tttgtgtgac cgatccggtc tatcaggtac ttgcttatga tacaggagga
    19861 aaatggagtg ggaatggtgt caatgccaaa ggggaaatcg accccaaaaa attaggcccg
    19921 ggcacattct ctgctatcta tacaaccgat aatggtgttt gtaaaacaaa agatgaagta
    19981 ttgttcaagg taaatccttt gcctaatgtc aaaataacag acccgggacc gatatgtaat
    20041 gataccattc tgataaccct taaaggctca ccagcaaagg gaaaatggag tggtttaccg
    20101 gattcactcg gtaagtttaa tcccaacatt actaagcccg gtacatataa ggtcttctat
    20161 agctatactg atttatttgg ctgcaaaaat aaagattcgc ttaatataaa aattaatcct
    20221 gcaccttttg ccgtaattac acctagtgca caggtatgta acagcaacca aaacggcaat
    20281 acatcattac ttgattttag tttactgatt tctgcaggcg atgcaggtgg cagctggcag
    20341 gatattgatg gttcaggggc cacaggtaca tttcctgtcc ttgatttttt aaatgctgca
    20401 cccggcaaat acaacttcac ttatacaacc aattctgccc taccgggctg tgatgaagtt
    20461 agctatacaa tagaagtaga agtagtggac tgcaaatgtc cggctttgtt tttaacaccg
    20521 tcaaccatac tatgcaatga cctggccaac tacgatctca gtaatctaaa attaaatact
    20581 aacgacggca cttggtcaat aataaacact cctccgggat caaatcctgc ccttataaca
    20641 ggaaataaca ttaatattca aaacaaagat accggaaatt acactatcga gttcaaactt
    20701 gtaaccactc cgcctccagg atgcgataac accaacacta cagaaataac acttcatgca
    20761 ccgccggttc ttacttttgc tccgactaag acagtttgca acaagatatt gccaggtagt
    20821 tctccagtca cacttgattt ggacacttac atcacttctc ctgacaaatc tggtatctgg
    20881 acagaaataa cttcctctgg tgcatcgggt accatgccat tccttgattt taccggtgta
    20941 acacccggca catatacttt ttcttatact accaattcag ctctggcccc ttgtactgaa
    21001 accacaaaaa cagttgatgt cattgttcag gattgtgaat gtccaagtct tgctttgaca
    21061 aagcaactcg aactatgtaa tgatggtacc acatacgact taaacctcca aaaaattaca
    21121 accgaaccag gaagctggac cttaaagcaa acacctccgg gagtaaatcc tatttcattg
    21181 actggttcca ttatttcgat ttcaaataag gatgccggaa attatgtttt cagctttaca
    21241 cttgccaata atccacctgc cggttgtgca aactctgatg acataactgt aagcctcttt
    21301 gattcgcctg aggcaacggt attatcaact gccgaggttt gtaacagcaa ctctagtggc
    21361 aatacgacta tattgaattt taatgatttc attatttccg gttcaaaaac aggtgtttgg
    21421 cttgatttga atggttcagg agctagtggt agcttcaatt caaaggattt tacgggtgta
    21481 atgcctggca actatacgtt ttcttataca accaacacgg ccaatttccc atgtacggac
    21541 aaaacctaca acatcatcat tacggtgaat gactgtaact gtcccgggct tggccttatt
    21601 aaatctacca gcatctgtaa tgaaggcaat acccaaatcg acctcaataa tctaaagata
    21661 acatctgaat ccggaaactg ggcggtcaaa tccaaaccta cgggtaatcc tgctaattta
    21721 acgggttcat tatttgatgt cagtaatgcg gcaactggca attatgtgat tacatttaca
    21781 cttcaaaata ctccacctcc agggtgtccg ttgtctgcag acctgaccat tgaagtattg
    21841 gcaccaccag tagccactct gaataacact gtttcggttt gcaattccac cggttccggc
    21901 cagtattcta ctgtattgaa tctgtataat caaataactg ccggtgataa aagtggtagt
    21961 tttgaagata ctgataattc tggcgcaaag cccattggca accagcttga ttttaccgga
    22021 gttgcgcctg gtaattatag ctttac
//
LOCUS       DAOQQY010000017        21908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_709_length_21908_cov_5.758935, whole genome shotgun sequence.
ACCESSION   DAOQQY010000017 DAOQQY010000000
VERSION     DAOQQY010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21908
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_21908_cov_5.758935"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..973)
                     /locus_tag="PLE23_01970"
     CDS             complement(77..973)
                     /locus_tag="PLE23_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82061.1"
                     /translation="MKIRIFLFAALIVSGYVANACDVCGGGGGGSLFGILPQYQKHFI
                     GFRYKYSAYQSTHHDESTYGNDYMNTAELWGRYSVTDRLHFYAMVPYQFVKRDEDGVI
                     STMNELSDITVLANYILLDNSRNAQPLVKHIFQLGGGIKLPTGKSDIVRNFQSLPENL
                     QPGTGTFDYLMTAVYTMRIKNYGLNADINYKYNTTNNRHYRQGDRFNASARFFAWYNY
                     GSISFLPNVGLDYEHADIDTKRSHDVEMTGGKSVFTGIGADVYYKSFAAGCRVQNAVY
                     QHLNEGNTTGKWRVNAQILYLF"
     gene            complement(1013..1393)
                     /locus_tag="PLE23_01975"
     CDS             complement(1013..1393)
                     /locus_tag="PLE23_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82062.1"
                     /translation="MIRRVGAILLLTLFLVQSSSKILIVSMWWARMDYIAENLCINKL
                     KPYLHCNGKCYLFQKLKEAEQKQRDQMPQLKNFQEMVYTYSPVPKVVECIFCQDEGDE
                     SLFAEMTLLRSQISTTDQFIPPEV"
     gene            complement(1488..2201)
                     /locus_tag="PLE23_01980"
     CDS             complement(1488..2201)
                     /locus_tag="PLE23_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMX82063.1"
                     /translation="MSEIKPTRTMRQWTRIKGENSWTLFKVLAEFVEGFDRLNEIGPC
                     VSIFGSARTQPDNLYYDLAVEVSRLLTEEGFGVITGGGPGIMEAGNKGASLYGGVSVG
                     LNIDLPFEQSHNPYIDYDKNLKHRFFFVRKVMFVKYAQAFVVLPGGFGTLDEMFEVLT
                     LIQTKKITRVPVVCVGTKYWSGLKEWIKDAMLTQSSNINPDDLKLFDITDDPAEVVKI
                     ITEFYATERQGQLSPNYEL"
     gene            complement(2295..3569)
                     /locus_tag="PLE23_01985"
     CDS             complement(2295..3569)
                     /locus_tag="PLE23_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMX82064.1"
                     /translation="MAKILIVDDEQSIRRTLKEILEFEKYIVDEAADGYDCLVKLKQD
                     KYDVIILDIKMPKLDGMDALDRIKELAQDIPVIMISGHATIDTAVESVKRGAFDFISK
                     PPDLNRLLITIRNALDRSTLMSETKTLRRKVQRSGVQEIIGESKAIKKIQTTIDRIAP
                     TDARVLVTGPNGTGKELVARWIHEKSQRTDNPIIEVNCAAIPSELIESELFGHEKGSF
                     TSAIKLRVGKFELANGGTLFLDEVGDMSLSAQAKVLRALQENQITRVGGDKSIHVDVR
                     VVAATNKDLREEIVRGRFREDLYHRLAVIIVDVPSLTDRLEDIPLLVEHFVENICAEY
                     KVPPKKVTPDAIKALQKYPWTGNIRELRNVVERLIILSGDSITEEDVLDYVIPAKSSG
                     LAKFKEIFDKFDSLDEMHQYIEKEYYRYKAVS"
     gene            complement(3943..5148)
                     /locus_tag="PLE23_01990"
     CDS             complement(3943..5148)
                     /locus_tag="PLE23_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HMX82065.1"
                     /translation="MANELHLKELIREKAAGLLPDIIKIRRHLHAHPELSFEEFKTSE
                     YIRNILTGQQIKFTEQWVRTGIVAEVGNTENYVQTIALRADIDALPIQETNDVPYKSV
                     NAGVMHACGHDVHTSCLIGALIILNSLEVKWKNRTIGIFQPGEEKLPGGASLMIGEGL
                     IDNYKPEAIFGLHVLPQMEASNVGICPGKSMASSDEIYITIQGIGGHAAMPHLAIDPI
                     LIAADVLSGIQKVVSRHANPMIPTVLSFGKINSIGGATNVIPDEVKIEGTFRTMDENW
                     RREAHQIMRTYIQNTAEASGGKADVDIRKGYPCLINDDRLYAYGQQKLMELLDKNHVL
                     DVPARMTSEDFAYYSQQIPSLFFRLGTGNRLKKITSPVHTSSFDADESALETGMAAMA
                     YLAACKMME"
     gene            complement(5153..7114)
                     /locus_tag="PLE23_01995"
     CDS             complement(5153..7114)
                     /locus_tag="PLE23_01995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX82066.1"
                     /translation="MKLSVIIVNYNVRYFLEQALLSVRKAASGIDHEVWVVDNNSVDG
                     SVEMLESKFPEVKIIINTTNTGFSRANNQAILQSKGEYVLLLNPDTVVGEDTFLKCIQ
                     KMDEDQEIGAIGVRMIDGGGNYLPESKRGFPSPMTAFFKTTGLYRLFPKSGYFNKYYH
                     GDLNPGDNHYIEILSGAFMFIRRDALDKAGLLDEDFFMYGEDIDLSYRILKAGYKNYY
                     LADTTIIHYKGESTKKGTLNYIKTFYQAMIIFANKHFKGSSASVYIAFIRMAVFLRGF
                     LALLSGFVSKYRYLIMDSFLLFVSLMVCKEFWSVYRYDNPDYFPHHFTYINVPIYVSI
                     WVIAIYFNKGYERPDGYFRLFRGLALGTVVIAAIYGFLDLEYRNSRAVIILALLVGVA
                     LLSIYRVLAVSLRLKRFAVHFPVHASILVVGNQEESNRLKKLMSNGLQQKSFVGYVAP
                     VKTIDDNDCLGEITNLPLIATMYRINEIIFCAKDIHTTDMMNWMSAIGPSVTYKILPT
                     EGSFVIGSHSKESLGELYTFEISLKINQKPFQVQKRILDFVLSAILLLLSPVLLMFYK
                     DSGKLRSNLLKVLTGKLSFVGYANPEDATDLPNQKNGILTPLDELNASADDKKLIQSL
                     NFMYAKDIDIFRYLQIIFKGFNKLNRQTN"
     gene            complement(7120..7734)
                     /gene="recR"
                     /locus_tag="PLE23_02000"
     CDS             complement(7120..7734)
                     /gene="recR"
                     /locus_tag="PLE23_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMX82067.1"
                     /translation="MNPNSSKLLSDAVDAFTSLPGIGKKTALRLAMHLLNQDKFYTQR
                     ISEALLNMRTNIRRCKKCNNISDEDTCSICSSPSRKNSVLCIVESIRDVIAIEDTQQY
                     NGRYHVLGGLISPLEGIGPEDLNLHSIKERISSEDIQEIIMALNPTIDGDTTIFYLSR
                     LLKDTEVKITLLSRGVSFGGELEYTDELTLGRSILARVPYTIPD"
     gene            7871..9118
                     /locus_tag="PLE23_02005"
     CDS             7871..9118
                     /locus_tag="PLE23_02005"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HMX82068.1"
                     /translation="MAEVIRMPRMSDTMEEGVIVSWQKKVGDTVEPGTVLAEVETDKA
                     VMELESFNEGVLLHIVVESGPVKVNGVLAVVGQAGEDYKPALDAAGAGSSVPATKEVA
                     AAPSESPAVTATPTTVPVATEDTGRIKASPLAKSMASQAGVDLKAVKGSGDFGRIIKK
                     DVEEFIQSGSKTTSAPVAVSAPAYIAVASGQDVDIPLSQMRKTIARRLSESMFTAPHF
                     YLSMDINMDRAVEVRKLINESEGKKISFNDLVVKAVAMALRHHPVINSSWLGDKIRQN
                     GNINIGVAVAVDEGLLVPVTRNTDMMSLSQISDTVKQLAGKAKERKLQPDEMQGNTFT
                     VSNLGMFDIESFTAIINPPDSCIMAVGSIVQKPIVKNGQIVVGNMMKVMLSCDHRVVD
                     GASGARFLQTFKNYLEEPVRMLA"
     gene            9127..9492
                     /locus_tag="PLE23_02010"
     CDS             9127..9492
                     /locus_tag="PLE23_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMX82069.1"
                     /translation="MQKTKTLVMGASPNPDRYSNAAVRELTKNHHPVVAFGRRTGKVA
                     GIPIETELVHYEDIDTVTLYLNPANQRPFYEYILSLEPRRVIFNPGTENPEFISLLRE
                     YNIYPEIACTLVLLSLSSY"
     gene            9575..10882
                     /locus_tag="PLE23_02015"
     CDS             9575..10882
                     /locus_tag="PLE23_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMX82070.1"
                     /translation="MIKKILQNYGIDALNEMQEAMLKATKDHREVLLLAPTGSGKTLG
                     FLLPAYMGLKQSIKGVQVLIVVPSRELASQIEQVFKQMGTGFKVNAFFGGHSSRTEKN
                     SLIDPPAVLIGTPGRLSYHFRTEHIDPTTIHTVVLDEFDKSLEFGFSREMSTIMKHVR
                     YVKNKVLTSATGMEEFPDFLKMRKPYELNYLDEEEMKPSGLSFYKVDIINNDKTETLF
                     NLLCEIAGQPTLIFFNHRDAIGKTSELLTQKGIRHGVFQGGMEQEDREEALIKFRNGS
                     HTVLLCTDLASRGLDIPEIQNIIHFQIPHTVQAFTHRNGRTARMKAEGKAFFLVKSTD
                     RSPDYLEDEPEKFALEANPKLPPASEWITLRFPYGRKEKINKTDIAGLLYQKGKLSHD
                     ELGLIEVTDHRAYATVKSSKVSATIKLIKEERIKNKLVKVEIV"
     gene            complement(10941..11522)
                     /locus_tag="PLE23_02020"
     CDS             complement(10941..11522)
                     /locus_tag="PLE23_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMX82071.1"
                     /translation="MFESFLRYMRTYVDLTTEEEEILVSVMFFRKIKRKQFFLVEGEI
                     CRYQAYVIKGCFRTYYTDNEGRDVNLMFSIENWWMGDLASYFVSENSKCNIEALEDSE
                     ILILEKQNLDILFNKIPKMERFFRILFQNALISSQNRIINNLTLSAKENYLIFQKKYP
                     EFEQRIPQYHIASYLGITPEFLSQVRKQLSGRA"
     gene            complement(11673..12065)
                     /locus_tag="PLE23_02025"
     CDS             complement(11673..12065)
                     /locus_tag="PLE23_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMX82072.1"
                     /translation="MSAVHLNIGRDTYKTIIHTRGHEIIADEPTELGGHDLGFNPKEL
                     LAAALGACTSITLRMYADRKEWDIAAIETEVHVDWKKDELKTNFTRKINFTGNIDETQ
                     KERLLKIANSCPVHKILSGEIEIQTTVH"
     gene            complement(12132..12219)
                     /locus_tag="PLE23_02030"
     tRNA            complement(12132..12219)
                     /locus_tag="PLE23_02030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12183..12185),aa:Ser,seq:gga)
     gene            complement(12455..17731)
                     /locus_tag="PLE23_02035"
     CDS             complement(12455..17731)
                     /locus_tag="PLE23_02035"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMX82073.1"
                     /translation="MKANLYTNLVFVSFFLTVMLCAFPGQAQIKLEDFPKEHNIELIK
                     GKKRQLVLMPPGENTVKVNTMEVGEQYLVVFNIADHDSPCNPQYRLVCGSLDTVLNSK
                     EIRFLAKSTTATLYVNTGCPADYATRLALNISFMRDGGLIDVTKNTKPKPESVTVSSG
                     GSASYLITDIFIGGGCFEVSNVSTIGKAVQLGEFSGGGSSIQIETGVIIASGQAVTAS
                     GPNVGESSGSSVGGGGDGDLNTLANGSVFDAGGIQFDFKPTVNLVKFNYVFGSDEYNE
                     YTCSSFNDVFGFFISGAGIGGNKNIALIPGTNTPVKINNVNLGVVGSNGSMANCTPPN
                     GSLAYSGFFVNNPVGSPDVEYDGFTTVFTAEQVVIPCSTYHIKFAVGDVGDGVYDSGV
                     FLEANSFSAGGNASGTVIVPSSGTNVVYEGCADGYVEFCRTSSNGNDQDLVLSFTIEP
                     TSTATPGVDYAPFSSPIVIPAGEDCVELPITVFKDAITEGTESIKLKLSTPCSCESPF
                     IEILIKDSPPLVLTANDVTICEEEGGADISVTATGGVGDYTYLWNNGATDPSIFVGTS
                     TSQTYTVTVTDECGNSAQKDVKVIVIPSPEATIEGYGTVCYQQAGTVNLTVKFTGPGP
                     WTIIYAINGVPQPPISNITQNPYILKGSEAGFYTIEQVYGDFGCEGVGMGEGQIDEVK
                     VEMFVDIENPSCFGVQDGKITVDPYGGEENYYIKWSNGVEGFDYIDLLAAGSYKVTVT
                     DANGCKTVQTINVVQPPELKVTAVIQNVKDCKNPFGGGIDLTVTGGTLPYDFNWDNGL
                     PPDEDQSNLKSGIYIVTVTDANGCYGIYTYLITANDAPKVTAQTTKNVNCKDLNAGQI
                     QLNVTGGQGPYTYLWNNQATTKDISGLTFGDYAVTVTDAAGCETYATSSVSADTVKPV
                     VVTGPDLLLPCSVLQLTLDASATPMPNGFTAQWTTANGNIVSGANSLKAIVDAPGAYT
                     LTITNGNNGCSNSKTVIVDPDLAKPNVFVNPPVILTCDLTQLTLDGSASSQGSNYQVS
                     WTTAGGNIVSNPSGNYAIDINAPGKYTLKLINTTNGCDNSFTVDVKQDIDKPLADAGN
                     TVLLTCKETDLTLNGNGSSTGADFIYDWTTKNGFIVTGKTGLTPTVNQPGWYYILVKD
                     KTNGCTNLDSVLVNQDIVKPIAAAASPELLTCAVSVVPVSATGSSTGSTFSYDWSTVS
                     GSIQGTNNGFTVNATSIGSYTVTVTNSTNGCTQTATVVVNEDKEKPVAKAGTDLILNC
                     QQTTQLLDGSGSSVGPEFTYLWTTLDGNIISGANTLNPTIDQAGTYTLTVTSSKNGCS
                     SSDDVKISLSEDFPVAVVVDPEVITCVKLSVLINGLGSSTGPEFTYQWSTTTGNIVAG
                     ANSSTPEVDKAGVYLFTITNVANGCVTNKNVTVKEDKVKPKAVAGPDFEAGCFDVPEA
                     LDGTASSGKGTLTYNWTTTNGNIVSGENTATPLIQKAGIYSLLITDNSNGCTATDNIE
                     VTSNALEDVTFTLKIPSCFGEYGTAQIVNVKGGLPPYKYSINGGQSYTGNAVFNKLNP
                     GLYNLLVKDGYDCIIARSFEIPAKTDIQVLVEPKVELYLGESYTLKAQVNIPKNLIAK
                     VEWFSKDGLERPDSLVTKVNVLQTTEYIVRVTDISGCTGQARVLVVIADPEIYVPNAF
                     SPYDLNGNNDLAMVFGRPSGIKKITKFQIFNRWGESMYESSDFLPNDPNHGWNGLHRG
                     KKLDPAVFVWWLEVEYINGKKKVYKGDINLLR"
     gene            complement(17986..19830)
                     /locus_tag="PLE23_02040"
     CDS             complement(17986..19830)
                     /locus_tag="PLE23_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82074.1"
                     /translation="MVRVLLMVLFIFPVILLAGQDSIPPNKDARALTSDSSVIYQFRA
                     FSDQAEGVVDLYAIPDGIQQPDPIRQEGIYFGNLGNIGSSHKFLVYDAVPDITFDMGF
                     HAFDLYRLNVANAIFYKGYRPYTKVLFTQMGGQGNGIFRGTYGRELSKTLSLHLDFSR
                     MANLGYYQRQKIKHTGLTLGLWYQGMKNRYKAWLMYGSNYNNQQDNGGIATDTLFDNE
                     IYWRREAIPVRLLNADTRYVQYELSYKQLYSLVNPGKPGLFYLVHQSRYVKQTFKFSD
                     TYPNVDFYGPFYTYPKGLRYFSEVKGIQNYFGLQTGIASNRRSFVLEPGVSYKRYNIT
                     LDSTAYNWNELWLHGSLSGSVGPFSLLVQGQYGLLDALNSFRLSGEATGDIGKWATLS
                     AKLILQKYPGTSMMYGFNLSKLPIWEGSPEKTTELAIYANLEIKPVNLNIYARQFVLN
                     NYIYFDQQALVKQLAGAFTINQFGAEHRLKFWKIHFNNKIHFQTKSEDVIRFPEWITT
                     HSLYFDGKIFKNYLQIRPGIDLRYLSSYYADNYMPASGQFYLQDKTKLPDQWLTDVFL
                     GIGIANFQGFVKMENVTNWVKKGIQYYVPFYPQPEAKFRIGLQWQFLN"
     gene            complement(19834..20664)
                     /locus_tag="PLE23_02045"
     CDS             complement(19834..20664)
                     /locus_tag="PLE23_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMX82075.1"
                     /translation="MSNNLLKGKTGIIFGALDEQSIAWKVAERAVEEGARITLTNAPV
                     AMRFGEIYKLAEKLNAEVIPADATELADLEQLVDKSIESFGGQKIDFVLHSIGMSLNV
                     RKKKDYTDLNYEWMQKSLDVSAISFHKMMQVLYKKDALNDWGSILALTYIAAQRTFPD
                     YSEMAEAKALLESFARSFGYHFGVKKHVRVNTLSQSPTMTTAGSGVKGFTEFFQYADK
                     MSPLGNASALDCANYCVTMFSDLTRMVTMQNLFHDGGFSSMGMSPAILDWNPQANTGV
                     "
     gene            complement(20711..>21908)
                     /locus_tag="PLE23_02050"
     CDS             complement(20711..>21908)
                     /locus_tag="PLE23_02050"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMX82076.1"
                     /translation="IKTYETEFKEYVQAVKSLEELRSASDKQEAEQEYRQFLFDELEA
                     LHLTEGIQNMMESELAILENAELIKKNLNAAYQHLSESDNSIFQQLRDVNLLLQAVSK
                     FNDGIGGLSERLEQVQYEIEDIAVSLEQLADKTEFDPEKTNIFQTKLDEIYRLLKKHK
                     LQNSDQLMSMRNQLELQLNGEGDVGEMLAKLEKKIVELLAHLKSTGAEISKGRKSAAL
                     VLESKVNEMLQQLNMPNALLKVHLYESEQPGPHGLEQISYLFSANKGSVPAEIRTVAS
                     GGELSRLALSIQSLVASSIPLPTMVFDEIDSGVSGAVSLKVGNILRTLSDKHQVIVIT
                     HSPQIAAKANKHYHVYKTDIGTQTKTYVRELSVDERIASIAIMLSTDPPTKAALENAR
                     ELMEIS"
BASE COUNT         6291 a         4679 c         4317 g         6621 t
ORIGIN      
        1 aatagcgata tagtgaatat gcatgtcaat gcaatcattg aattcttaaa catgataatt
       61 tgtttataaa aattagttaa aataaataga gaatctgggc attcacacgc cacttgccgg
      121 tagtgttacc ttcattcagg tgctggtaca ctgcattttg cacccgacaa ccagcagcaa
      181 aacttttata atacacatcc gcaccaatgc ctgtaaatac acttttgcca cctgtcattt
      241 ctacatcatg gctacgtttg gtatcaatgt cggcgtgctc atagtccaga cctacattgg
      301 gcaggaatga aatactgccg taattatacc atgcaaaaaa tctggctgag gcattaaatc
      361 ggtcaccttg tctgtaatgc ctattgttgg tagtattgta tttataattg atgtctgcat
      421 tcaacccata atttttaata cgcatggtat atactgctgt catcaggtag tcaaatgttc
      481 cggtgccggg ctgaagattt tcgggtagcg actgaaaatt tctgacaata tccgatttgc
      541 cggtaggtaa tttaataccc ccaccaagtt gaaaaatatg cttgaccaag ggctgggcat
      601 tgcggctgtt gtctaaaagt atataattgg ccaatactgt tatatcacta agttcattca
      661 tcgttgaaat gactccgtct tcatcccgtt tgacaaactg atatggtacc atagcataaa
      721 agtgcagacg atcagtaacc gaatatctgc cccaaagctc agcagtattc atataatcat
      781 tgccgtaggt actttcatca tggtgggtac tctggtatgc actgtattta tacctgaacc
      841 ctataaaatg tttctggtat tgtggaagaa tgccgaagag gctaccacca ccaccaccgc
      901 cacatacatc gcatgcattt gccacatagc cggatacgat aagtgcagca aataaaaata
      961 ttctgatttt catttgataa aaaatttagc atgttaacaa aatgggtaac tgctaaactt
     1021 cgggaggaat aaattgatct gtggttgaaa tctgtgaccg gagcaatgtc atttctgcaa
     1081 aaagtgattc gtcaccttca tcctggcaaa aaatacattc aaccactttg ggtacaggag
     1141 aatacgtata aaccatttcc tgaaaattct tcaactgcgg catttggtcg cgttgctttt
     1201 gctctgcttc tttcagtttt tgaaaaaggt agcacttacc attgcaatgc aagtaaggct
     1261 taagtttgtt gatacagaga ttttcggcaa tgtagtccat ccttgcccac cacatcgaca
     1321 ctatcagtat cttggatgat gactggacaa gaaaaagtgt tagtaataat atggcaccta
     1381 ctctgcgaat cataatgtaa aggtaaggag gcttttacaa ttcatttatg actaaaatca
     1441 taagtgatac ctcgaagtta tgattttcat tactttccta aaaaaaatta aagttcgtaa
     1501 tttggtgata gctgcccctg gcgttctgta gcataaaatt cagtaattat cttgactacc
     1561 tctgccggat catccgttat atcgaataat ttcagatcat cggggttaat atttgaagac
     1621 tgggtaagca ttgcatcttt gatccattcc ttcagtcctg accagtattt ggtaccgacg
     1681 catactaccg gtacacgtgt tatcttcttg gtctgaataa gcgtaagtac ttcgaacatc
     1741 tcgtcgaggg tgccaaaacc acccggtagc accacgaaag cttgtgcata ttttacaaac
     1801 atcacctttc gcacaaaaaa gaaacggtgt ttgaggtttt tgtcgtagtc gatatatgga
     1861 ttatggcttt gttcgaatgg aagatcaata ttaagaccaa cagatacgcc accatacaga
     1921 gatgcacctt tgttgcccgc ttccataata cccggaccac caccggttat aacgccgaat
     1981 ccttcttcgg ttagtagtct ggatacttct acagccaaat catagtatag gttatcgggt
     2041 tgagtacgtg cactaccgaa tatagacaca cagggtccta tctcatttag tctgtcaaac
     2101 ccttctacaa attctgcgag aaccttaaac aaagtccacg aattttcgcc ttttattctg
     2161 gtccattggc gcattgttct ggtcggctta atttcgctca tgtcatatct ttttgattat
     2221 caaatattta aacagcacaa agcccatctg gggagacggg ctttatcaaa tgaaacagaa
     2281 agttataaaa aaagttacga aacggcttta tatctgtaat attctttttc aatatattgg
     2341 tgcatttcat ctaaactatc aaatttgtcg aatatttcct taaatttggc aaggcccgat
     2401 gactttgctg gaattacata atcgagtaca tcttcttctg tgatactgtc accactcaag
     2461 atgattaggc gctccactac attccgcagt tcacgtatat tccctgtcca tggatatttc
     2521 tgcaaagctt tgatggcatc aggtgttact ttcttgggtg gcactttgta ttctgcacaa
     2581 atattctcta caaaatgctc taccaacaac ggaatatctt caaggcggtc agtaagggat
     2641 ggcacatcta caataatgac tgccagcctg tgataaaggt cttcacgaaa tctgccgcgg
     2701 actatctctt cacgaagatc tttattggta gccgctacca cacgtacatc tacatgtata
     2761 gatttgtccc cacccactct ggttatctgg ttttcttgca gtgcccgcag tactttggct
     2821 tgtgccgaca aactcatgtc acctacttcg tcaagaaaaa gtgtcccacc gttggcaagt
     2881 tcaaatttac cgactctgag tttgatggct gatgtaaacg aacccttttc atgcccaaac
     2941 aattcgctct ctatcagttc gctcggtatg gccgcacaat tgacttcaat aatcgggttg
     3001 tcggtgcgtt ggcttttttc atgaatccat ctcgctacca attctttgcc tgtaccattg
     3061 gggccggtaa cgagtactct ggcatccgta ggcgctattc tgtctatagt ggtctgaatt
     3121 ttctttatag cctttgactc gccaatgatt tcctgcacac ccgatctttg aacctttctt
     3181 ctgagcgttt ttgtctccga cataagcgtt gatctgtcaa gtgcattgcg gatggtgata
     3241 agcagacggt tgagatcagg cggttttgaa ataaaatcaa atgccccacg ctttacactt
     3301 tctactgcag tgtctatggt agcatgaccg gatatcatta tcaccggtat atcttgtgcc
     3361 aattctttga tacggtcgag tgcgtccata ccatctaact ttggcatttt aatatcgaga
     3421 atgatgacgt cgtatttatc ctgttttaac tttaccaggc aatcataacc gtcagcggct
     3481 tcatcgacaa tatatttttc gaactccaat atctccttca gggttcttct tatactctgc
     3541 tcatcatcta ctattaatat cttagccatt attgaagctt gtatttgaaa attaataaat
     3601 taaacatttt actaatacgt tatttcgtca tgtgagtttc aaaaaactct agcatcagtt
     3661 ttatttaatg ttaaaattaa tgcgttacat attggttatt aatattttac aaatttatta
     3721 aaataaaata ataaaatcaa gtaatgtaat gaaaatgatt gttgtcctca actacagaat
     3781 gtgcatagga agcaacttat attatacagg gcaacattct tggcaaaaca ttaatatttt
     3841 tctgactaca ttatcataag acaatcagta aattgtgacg aaactccaga aattgaatat
     3901 tttggatact tacagagagt ataaaatttt ttatgaaatt ttttactcca tcatcttaca
     3961 ggcagcgagg taagccatag cagccatgcc ggtttcgagt gctgattcat ctgcatcaaa
     4021 agacgatgta tgcaccggtg atgtgatttt tttcagacga ttacccgtac ccagcctaaa
     4081 aaacaatgaa ggtatttgct gtgaataata ggcaaagtct tctgatgtca tcctggccgg
     4141 tacatccaac acatgatttt tgtctaataa ttccattagc ttttgttggc cataagcata
     4201 caaacggtca tcatttatca ggcacggata gccttttctt atatccacat ctgctttacc
     4261 acctgaggct tcagctgtgt tttgtatata tgtgcgcatg atttgatgtg cttcacggcg
     4321 ccagttctcg tccatggtac ggaaggttcc ttcgatcttt acttcatcgg gaatgacgtt
     4381 agtggcaccg cctatcgaat tgatcttgcc aaaactgagt actgtaggaa tcatcgggtt
     4441 agcatgccgg ctcaccactt tttggatgcc tgaaagtaca tcagctgcta tgagtattgg
     4501 gtcaatagcg agatgtggca tggcagcgtg tccgcctata ccttgtatgg ttatgtatat
     4561 ttcatctgat gaagccatac ttttgccggg gcaaatgcct acattactgg cttccatttg
     4621 gggcaataca tgtaatccga atattgcttc cggcttataa ttatctatga gaccttcgcc
     4681 aatcattaga cttgctccgc ctggcagctt ttcttctccg ggttgaaaaa taccgatagt
     4741 acggtttttc cattttactt caaggctatt gagaataatg agtgcgccaa tgaggcagct
     4801 cgtatgtaca tcgtggccac acgcatgcat aacacccgca ttgaccgact tataaggcac
     4861 atcatttgtt tcttgtatag gtaatgcgtc tatatctgcc ctcagtgcaa tggtttgtac
     4921 ataattttcg gtattaccta cctccgccac tattccggta cgcacccact gttctgtaaa
     4981 ttttatctgc tgaccggtaa gtatattccg gatgtattct gaagtcttga attcttcaaa
     5041 tgagagttcg ggatgagcat gtaggtgcct cctgatttta ataatatcag gtagcaatcc
     5101 ggctgccttt tctctgataa gttccttcag gtgaagttca ttggccatag tattaatttg
     5161 tttgccggtt aagtttgttg aagcctttga atattatctg cagatacctg aatatgtcga
     5221 tatctttagc atacataaaa ttaaggcttt gtatgagttt tttatcatcc gcgcttgcat
     5281 tcaattcatc aagcggtgta agaatgccat ttttttgatt aggaaggtcg gttgcatctt
     5341 cgggattggc ataacctaca aaagacaatt tcccggtgag tacctttagt aaattgcttc
     5401 taagctttcc ggaatcttta taaaacatca ataaaacagg actgagtaag agtaagattg
     5461 ctgaaagcac aaaatccagg attcgttttt gaacctgaaa aggtttttgg ttaatcttga
     5521 gcgatatttc aaaagtatat agctcgccaa gagattcttt cgaatgactg ccaatgacaa
     5581 aactgccttc agtaggcaat attttataag taactgatgg ccctattgcc gacatccagt
     5641 tcatcatgtc ggtggtgtgt atatcctttg cacaaaagat gatttcattg atccggtaca
     5701 tggttgctat cagtggcaga ttagttattt cgccaagaca gtcgttatca tcgatggttt
     5761 ttacaggggc cacatagcca acaaatgatt tttgttgcaa accattgctc atcaactttt
     5821 tcaacctatt gctttcttcc tgattaccga caacaagtat gcttgcatga acgggaaagt
     5881 gtactgcaaa gcgtttcaac ctaagactta cagccaagac acggtaaata ctcaacaatg
     5941 caactcccac caaaagtgcc agaataatga cagcacgtga attgcggtat tcgaggtcaa
     6001 gaaatccgta aattgcagct ataaccactg taccaagtgc caggcctctg aacaatctga
     6061 aatatccgtc cgggcgttca tagcctttat tgaaatatat agcaataacc cagatggaaa
     6121 catatattgg cacattgatg tatgtaaaat gatgcggaaa ataatcaggg ttatcatacc
     6181 ggtaaacaga ccagaactcc ttacaaacca tgaggctgac aaataacagg aaggaatcca
     6241 ttatcaggta tcggtatttg gaaacaaagc ctgataacaa ggctaaaaaa cctctcagaa
     6301 agacagccat cctgataaat gcaatataaa cacttgccga actgccttta aagtgtttgt
     6361 tggcaaagat aatcattgcc tgatagaatg ttttgatgta gttaagggtg ccctttttgg
     6421 tgctttcacc tttatagtga ataattgtag tgtcggccag ataataattt ttgtatcccg
     6481 ctttcaaaat ccggtacgaa aggtcgatgt cttcaccata cataaagaaa tcttcgtcca
     6541 gtaggcctgc tttatccagt gcatcccgtc tgataaacat aaatgcacct gagagtattt
     6601 caatataatg attgtcgccc ggattgagat caccgtggta atatttatta aaataacctg
     6661 attttggaaa cagcctgtat aaaccagtag ttttgaagaa agcggtcatg ggagacggaa
     6721 atccgcgttt tgattctggt agataattgc cgccgccgtc tatcatcctc acaccgatgg
     6781 caccaatctc ctggtcttca tccatttttt gaatacattt caagaaggta tcttctccaa
     6841 caacagtatc cggattgagc aacagaacat attcaccctt tgattgcagg atggcctgat
     6901 tattggctct tgaaaatccc gtattggtag tattgattat aatttttacc tcggggaatt
     6961 tactttccag catctcaacc gagccatcaa ccgaattatt atctaccacc catacttcgt
     7021 ggtcaatacc tgaagcagct tttcggacag acaagagtgc ttgttcgaga aaataacgga
     7081 cattataatt gacaataatg acagaaagct tcatagattt taatcaggga tggtataagg
     7141 aactcttgcc agaattgaac gcccgagagt gagttcatcg gtatattcaa gttcgccacc
     7201 gaatgaaaca cctcttgata acagtgtaat tttcacctcc gtatctttca acaaccgtga
     7261 gagataaaaa atcgtggtat ctccatctat tgtcggattc agagccatga ttatttcctg
     7321 tatgtcttca cttgaaattc tttccttaat gctgtgtaaa ttcaagtctt cgggcccgat
     7381 accttcaagt ggcgaaatga gtcctcccaa cacgtggtat ctgccgttgt actgctgtgt
     7441 gtcttcaatg gcaattacat cgcggatact ttcaacgata cacagtacac tattcttgcg
     7501 cgatgggctg gagcatatag agcaggtatc ttcgtccgag atattgttgc attttttaca
     7561 cctccggatg ttggtacgca tatttagcaa agcttctgat atgcgctgtg tataaaattt
     7621 atcctgattg agcaaatgca tagcaaggcg gagtgctgtt ttcttaccga taccgggcag
     7681 actggtaaaa gcatcaacag catcactgag taacttggaa gaattaggat tcataatgcg
     7741 caaaattaca aataaggtat caaaatcagg ttaaccggct gtgcaattaa aagtttattt
     7801 taaaatatag gctgtaattt taaaattagg agaatattcc gcatttttgc taaaagaaat
     7861 aagaacgaat atggctgaag taataagaat gccccggatg agcgatacaa tggaagaagg
     7921 tgtaatcgtt tcatggcaaa aaaaggttgg tgatacggta gaaccaggca ctgtattggc
     7981 tgaagtagaa accgacaaag ccgtcatgga gcttgagagc tttaatgagg gagttttgct
     8041 gcacattgtt gtagaaagtg ggccggtgaa ggtcaatgga gtactggctg ttgtaggaca
     8101 agccggtgaa gattataagc ccgctttgga tgctgccggt gctggttcat ccgttcctgc
     8161 aacgaaagaa gttgcagcag cgccatccga atcaccagct gtaacagcta ccccgactac
     8221 tgtacctgtc gcaactgaag atacagggcg tataaaggca tcacctctgg ccaagagtat
     8281 ggcctcacaa gcaggtgttg atcttaaagc tgtgaaaggt tcaggtgatt ttgggcgaat
     8341 cataaaaaaa gatgttgaag aatttataca aagcggaagt aagactacat cagctcctgt
     8401 tgcagtttcg gcacccgcat acattgcagt agcttcgggt caagacgtag atattccgtt
     8461 gtcacaaatg cgaaaaacca ttgcacgaag gcttagtgag agtatgttta cagcacctca
     8521 cttctatctt tcaatggata taaacatgga ccgtgctgtg gaagtgcgta agctcatcaa
     8581 tgagtcggaa ggaaagaaaa tatcattcaa tgatttggtc gtgaaggctg tagctatggc
     8641 cctaaggcat catcctgtaa tcaactcaag ctggctgggc gataaaatac gccagaatgg
     8701 caatatcaat ataggtgttg ctgtagctgt tgatgaagga ctattggttc ctgtgacacg
     8761 aaatacagat atgatgtcac tgagccagat tagtgatacg gtcaaacaat tggccggaaa
     8821 ggcaaaagaa agaaaactcc agccggacga aatgcaaggg aatacattta ctgtgtcaaa
     8881 ccttggtatg ttcgatatcg aatcgttcac agctatcatc aacccaccag attcttgtat
     8941 catggccgtt ggctctatcg tacaaaaacc aatagtcaaa aacgggcaaa tagtagtggg
     9001 caacatgatg aaggtaatgc tcagctgcga ccaccgtgtt gtagatggtg cttccggtgc
     9061 cagattttta caaacattta aaaattacct cgaagagcct gtgcgaatgc tcgcctgata
     9121 ccgaatatgc aaaagactaa aacactggtg atgggagcat ctcccaatcc ggacaggtac
     9181 agtaatgctg ctgtaagaga attgaccaaa aatcatcacc cggtggtggc ttttggtcgc
     9241 agaacaggaa aagtagccgg cataccaatc gaaacagaat tagtgcatta tgaagatatc
     9301 gatacggtga cgctttatct taatccggcc aatcagcgac cgttttatga gtacatactg
     9361 tcactagaac cacgaagagt aatatttaat cccggaacag aaaatcctga attcattagt
     9421 ctgcttcgtg agtacaatat atatccggag attgcctgta cactggtatt gctttcattg
     9481 agcagctatt gatccggaat ggcatccttc accaaagtca aatttttctg aagtgtaacc
     9541 agtcaaattt gcataaaaga attatgcggc agctatgata aaaaaaatac ttcagaatta
     9601 cgggatagat gcgctcaatg agatgcagga ggctatgcta aaggctacaa aagaccatcg
     9661 cgaggtactc cttctggctc ctacaggctc gggcaaaacg cttggttttc ttctgcctgc
     9721 ctatatggga cttaagcaga gtataaaggg cgtccaggtg ctgatagttg taccatccag
     9781 agaacttgcc tctcagatag aacaggtatt caaacaaatg ggcacaggtt ttaaggtcaa
     9841 tgcatttttt ggcgggcact ctagtcgtac tgagaaaaac agtctaattg atccaccggc
     9901 tgttcttatc ggcactccgg gaaggttgtc ttatcatttc agaacagaac acatcgaccc
     9961 gactaccatc catacagttg tactggatga atttgacaaa tcacttgaat tcgggttcag
    10021 ccgtgagatg agtaccatta tgaaacacgt caggtatgta aaaaataaag tacttacttc
    10081 tgccaccgga atggaggaat tccccgattt cctaaaaatg cgcaaacctt atgaactcaa
    10141 ttatctcgat gaagaagaga tgaaaccatc aggtctcagc ttttataaag tcgatatcat
    10201 taataatgac aaaactgaga ctctctttaa cctgctttgt gaaattgccg gacagcctac
    10261 gcttatcttc ttcaaccacc gagatgcaat aggtaaaacg agcgaactcc ttacgcaaaa
    10321 aggtattagg catggtgttt ttcagggtgg tatggaacag gaagaccgcg aagaagcgct
    10381 catcaaattt cgcaatggca gtcacaccgt cttgctctgt accgatctgg catcgcgagg
    10441 gctcgatatt ccggaaatac aaaatatcat tcattttcaa ataccgcata cggttcaagc
    10501 attcacccac cgtaatggcc gtactgccag aatgaaagct gaaggtaaag cattctttct
    10561 ggtaaaatct acagaccgta gccctgatta tctcgaagat gaacctgaaa aatttgcact
    10621 cgaagcaaac cctaaactac cgccggcttc tgaatggatt acactgcgtt ttccttatgg
    10681 ccgcaaggaa aaaatcaata aaactgatat tgccggactg ctttaccaaa aaggcaagct
    10741 cagccatgat gaactcggtc tgatagaggt gaccgaccac agagcctatg ctactgttaa
    10801 atcgagcaaa gtatcagcca ccatcaaact catcaaggaa gaacgcatca agaacaaact
    10861 cgtaaaggtt gagattgtgt aacaatggga ataagttggt tttgacgtaa aatattttca
    10921 aaaattatca cactaataac ctatgcccta cctgatagct gctttcgtac ctgactcaga
    10981 aactcaggtg tgataccaag ataagacgca atgtggtatt gtggaatgcg ctgttcaaac
    11041 tcaggatatt tcttttgaaa tataagataa ttttcctttg ccgatagggt aagattattg
    11101 ataatcctgt tttgtgatga aatgagtgca ttctggaaca gtattctaaa aaaccgctcc
    11161 atctttggaa ttttgttaaa taagatgtca agattttgtt tttctagaat aagaatttcg
    11221 ctgtcttcaa gcgcttcaat gttgcatttt gaattttctg agacgaaata actggcgagg
    11281 tcacccatcc accagttttc aattgaaaac atcagattga cgtcacggcc ttcattgtct
    11341 gtataatatg tacgaaaaca acctttaatg acataggctt ggtacctaca aatttcacct
    11401 tctacgagaa aaaactgttt tcgtttgatc tttctgaaga acattacaga aacgagtatt
    11461 tcctcctctt cggtagtcaa atctacataa gtgcgcatat atcgcagaaa ggattcgaac
    11521 attgcagtat ttaataatca gaaaaataat gcattctata cctgaatagt tttaccttga
    11581 tacaattgct gctaaaaata catcttttgt agaattttta caaaactaat gtaaaaatat
    11641 atccctatat ttaatcagat agctggaaat ctctaatgaa cagtggtctg gatttcgatt
    11701 tcgccactca gtatcttatg taccgggcaa ctgttggcta ttttaagtaa acgttctttt
    11761 tgggtttcgt caatatttcc ggtaaaatta atttttcggg taaaatttgt ttttaactca
    11821 tcttttttcc aatctacatg tacttctgtc tcgattgctg ctatatccca ctctttgcgg
    11881 tcagcataca tacgcaaggt aatactggta caagcaccga gtgctgctgc caataattct
    11941 ttcggattga aaccaaggtc atgaccacca agctccgtag gttcgtctgc gataatctcg
    12001 tgtccgcgag tgtgaatgat cgttttgtat gtgtctcgac caatatttaa gtgaacagct
    12061 gacataatat tgctaaagga tttttaatat tgatatattg tagaaaacaa tgaggtatga
    12121 aaaaaccaat gcggagagtg agagattcga actctcggta cacttttggc gtacacacac
    12181 tttccaggcg tgctccttaa accactcgga caactctccg taagctttaa aatttcaagt
    12241 tgcaaaaata caagataaat tgacaaaaca aaaagcattc tataattgat tctttcaaat
    12301 ttggatgtag ttatagtcta aaaatatgaa ctgccttctc ataaaaatgg cagaacaact
    12361 ttatttctgt taactcttcg aaaaaatact tttcaaccaa gtcaagactt aaagctattc
    12421 tgcccaaaac agaacaaatg tttatttaac cattttatct caataggtta atatctcctt
    12481 tatatacttt ctttttgcca ttgatgtatt ctacttccaa ccaccataca aacactgccg
    12541 gatcaagttt cttacctctg tgcaatccgt tccagccatg attagggtcg ttaggtagga
    12601 agtcagatga ttcgtacatt gattctcccc acctattgaa tatctggaat ttggtaattt
    12661 ttttaattcc actcggccga ccaaagacca ttgccaggtc gttatttcca ttcaggtcat
    12721 aaggtgaaaa tgcattcggt acatatattt ccggatcggc tattactacc agaactctgg
    12781 cctgaccggt acatccgcta atatctgtca cgcgcactat atactctgtt gtttgaagca
    12841 cattcacctt tgtcacaagt gaatctggtc tttcaagccc atcttttgag aaccattcca
    12901 ccttggcaat caggtttttc ggaatattaa cctgggcttt aagtgtatat gattcgccaa
    12961 gataaagctc cacttttggc tctaccagta cctgtatgtc tgttttagcc ggtatttcga
    13021 aactacgtgc aataatgcaa tcgtatccat ctttgaccaa gagattgtag agtccgggat
    13081 tcaatttatt gaacactgca tttccggtat agctttggcc accgttgata gaatacttgt
    13141 aaggaggcaa accgcccttt acgtttacaa tctgtgcggt accatactca ccaaaacagc
    13201 ttggtatttt caatgtgaat gttacgtctt caagagcatt gcttgttact tcaatattat
    13261 ctgtagcagt acagccattg ctattatccg ttatcagcaa tgaataaatg ccggctttct
    13321 gaattagcgg agtagctgta ttctcaccac ttacaatatt accgttggta gttgtccagt
    13381 tataggtaag tgtacctttg ccacttgatg cagtaccatc cagtgcttcc ggaacatcaa
    13441 aacaaccggc ttcgaagtca ggacctgcga cagcctttgg ttttacttta tcttctttga
    13501 ctgttacgtt cttattagtt acacaaccat ttgcgacatt tgtaatggta aacaaataaa
    13561 caccggcttt gtccacctca ggagtagaac tgttagcacc tgctacgatg ttaccagtag
    13621 tagttgacca ctggtaggta aactctggtc cggtagaaga ccccaaaccg tttattaaaa
    13681 ctgacaattt tacacaggta atcacctccg gatcaacgac tactgctaca ggaaaatctt
    13741 cacttagtga aatttttaca tcatctgatg aagaacagcc gtttttgctg cttgtaactg
    13801 tcaaggtata tgtacctgcc tggtctattg tcggattgag tgtattggca cctgaaataa
    13861 tgttaccatc cagtgttgtc cataggtaag tgaactcagg gcctacactt gaaccggagc
    13921 catcaagcaa ttgggttgtc tgttggcaat tgagtatcag gtctgttccg gctttggcta
    13981 caggcttttc tttatcttca ttaactacta ctgttgctgt ctgagtacat ccgttggtgc
    14041 tgttggttac agtaacggtg taggaaccaa ttgatgtagc attgacagta aaaccattgt
    14101 tggtgccctg aatactacct gatactgttg accagtcata actgaaggta ctaccggtgc
    14161 ttgaaccagt tgcactcaca ggcaccacac ttactgcaca tgtcaacaac tctggcgagg
    14221 cagctgcggc tataggtttc acaatatcct gattaaccag aaccgaatca agatttgtac
    14281 aaccattggt tttatcctta actaatatat aataccagcc cggttgattg actgtaggtg
    14341 tcagccctgt tttaccggta acaataaacc catttttagt agtccagtca tatataaaat
    14401 cagctcctgt actactcccg ttgccgttga gtgtcaaatc ggtttcctta caagtaagca
    14461 aaacggtatt gccggcatcc gccaatggct tatcaatatc ttgtttgaca tcaacagtaa
    14521 acgaattatc acatccgttg gtagtgttga taagtttgag tgtgtactta ccgggtgcat
    14581 tgatgtctat ggcgtaattt cctgacggat tgctaacgat atttccaccg gcagtcgtcc
    14641 aactcacctg ataattgctt ccctgacttg atgcagaacc atccaatgtc aattgggtga
    14701 ggtcacatgt aagtatgacc ggtgggttga caaaaacatt tggctttgca agatcaggat
    14761 ccacgattac ggttttagaa ttggagcatc cattattgcc gttggtaatg gtaagggtat
    14821 aggcacccgg agcatcaacg atagctttaa gagaatttgc tcccgaaaca atattgccat
    14881 tggctgtagt ccactgggca gtgaatccat ttggcattgg tgttgcactt gcgtcaaggg
    14941 taagctgtaa gacagagcaa ggcaatagta aatctggtcc ggtgactaca accggcttaa
    15001 ctgtatcggc acttaccgaa gatgttgcat aggtttcaca tccggcagca tctgtgaccg
    15061 ttacggcata atctccgaaa gtaagacctg aaatatcctt tgtggtcgct tggttattcc
    15121 acaaataggt atatggacct tgaccaccag tgacattaag ttggatttgg cctgcattaa
    15181 gatccttaca attgacattt ttggttgtct gtgcagttac cttaggtgca tcattggctg
    15241 ttattagata cgtataaata ccataacaac cattggcatc ggtcacagta actatataaa
    15301 taccggattt taagtttgac tggtcttcgt caggtggcaa tccgttgtcc cagttgaaat
    15361 cgtaaggaag tgtgcctcct gtgacagtca ggtcaatgcc accaccaaaa ggatttttac
    15421 agtctttaac attctgtatt actgctgtta cttttaactc cggtggctga accacattga
    15481 tggtttgtac agttttgcat ccgttggcat cggtgacggt aactttataa cttccggcag
    15541 ccagcagatc tatgtaatca aaaccttcaa caccattcga ccatttgata taataatttt
    15601 cttcaccacc ataaggatcg actgttattt taccatcttg tactccaaag catgaagggt
    15661 tttcgatgtc aacaaacatt tcaaccttta cttcatctat ctggccttct cccattccta
    15721 caccttcaca tccaaaatct ccataaactt gttcgatggt gtaaaaacct gcttcagagc
    15781 ccttcaaaat ataaggattc tgtgtaatat tactgattgg aggctgaggt acaccattaa
    15841 tcgcataaat aatcgtccac ggacccggtc cggtaaattt gacagtaaga ttcactgttc
    15901 cagcttgctg gtaacacacg gttccataac cttcaatggt agcttctggt gaaggaataa
    15961 ctataacttt tacatctttc tgtgccgaat tgccgcattc atccgtaact gtaacggtat
    16021 aggtctgaga agtgctcgta cctacaaaaa tacttggatc agttgcacca ttattccaaa
    16081 gataagtata gtcgcctact ccaccagtag cagtgacact tatgtcagca cccccctctt
    16141 cttcgcagat ggttacatca ttggcagtca atacaagtgg tggtgaatct ttgatcaata
    16201 tctcaataaa tggggattcg caagagcaag gggttgacaa tttaagcttg atagattctg
    16261 taccttcggt aatcgcatcc ttgaataccg tgatcggcaa ttcgacacaa tcttcacctg
    16321 ccggaatgac aatagggctt gaaaaaggtg cataatcaac acctggggta gcagtagaag
    16381 tcggctctat tgtaaatgac agtacaaggt cttgatcatt tccattacta cttgttctgc
    16441 agaattcaac atagccatct gcacaacctt catatacaac attcgtacca gaactcggta
    16501 caattacagt accactcgca ttaccaccgg cactaaacga gttggcttca aggaatacgc
    16561 ctgagtcata aacaccgtca ccaacatcgc ccactgcgaa tttgatatgg taagtgctac
    16621 aaggtataac cacctgctcg gccgtaaata cagttgtaaa accatcgtat tccacatcgg
    16681 ggctgcctac cggattattc acaaagaaac ctgaataagc caaggagcca ttgggcggag
    16741 tacaattggc catacttcca ttagaaccaa ctacccctag gtttacattg ttaatcttta
    16801 ccggtgtatt tgtacccggt ataagagcaa tattcttgtt gccaccaata cctgccccac
    16861 tgataaagaa accaaacaca tcattaaaac tagaacaagt atattcgttg tattcatcag
    16921 aaccaaacac ataattaaac tttaccaggt taacggtagg cttaaagtca aactgaatac
    16981 ctccggcgtc aaagactgat ccgttggcaa gtgtattaag gtctccatca ccgcctccac
    17041 caacactgga ccccgagctt tcacctacat ttggcccact cgccgttact gcctggccgc
    17101 ttgctatgat gacaccggtt tctatctgga tggaggatcc tccacctgaa aattcaccca
    17161 attggacggc cttacctatg gtgctcacat tcgacacttc gaaacaacca ccaccaatga
    17221 aaatatctgt aatcagataa gaggctgagc caccagatga tacagtaact gactccggtt
    17281 ttggctttgt gtttttagta acatcaatca aacctccatc acgcataaaa cttatattca
    17341 atgccagcct ggttgcataa tctgccggac aacctgtgtt tacataaagt gtagcagttg
    17401 tagatttagc caaaaatctt atttccttac tgttcaagac tgtatcaaga gagccacata
    17461 ccaaacgata ttgaggattg cacggactat cgtggtcggc aatattgaag acgacaagat
    17521 actgctctcc gacttccatt gtattgactt ttacagtatt ctctcccgga ggcatgagta
    17581 ccaactgcct cttctttcct tttatcaact ctatattatg ttctttagga aaatcttcaa
    17641 gttttatttg tgcctgaccg ggaaatgcac atagcattac agtcagaaaa aaacttacaa
    17701 acactaaatt tgtgtagagg tttgctttca tttttggtat aatttagact ttatcagcgt
    17761 taaagttaaa ataatgtatg ccatcatcca aatattttac aatttttttt accgacacac
    17821 attgatactt cacaccttac atctgatacc tttggtgtat gtttcgttgc ctgatgttaa
    17881 ttatttctta aaaaaactat ataatgaata tagttaaccg tggaatgact cacaataccc
    17941 atcggtttca ccggaaaata aagcttaaaa aattaaaatt gcgcctcagt tcaaaaactg
    18001 ccattggagg ccaatcctga attttgcctc aggttgtgga taaaatggta cataatattg
    18061 aatccctttt tttacccaat ttgtaacatt ctccattttt acaaaaccct gaaaattggc
    18121 aattccgatt ccaagaaaaa catctgtaag ccattggtca ggtaattttg ttttgtcctg
    18181 aaggtaaaat tgtccgcttg ccggcatata gttatcggca taataactgc tcaaataccg
    18241 gaggtcgata cccggcctta tctgaagata attcttaaat atttttccat caaaataaag
    18301 gctatgcgta gttatccatt ccgggaagcg gataacatct tctgactttg tctgaaaatg
    18361 aattttattg ttgaaatgta ttttccaaaa cttcaaacgg tgttctgcac caaattggtt
    18421 aattgtaaat gctcctgcca actgctttac aagtgcctgc tgatcaaaat atatgtaatt
    18481 gttgagaacg aactgtctgg cataaatatt gaggttgaca ggttttatct ccagattggc
    18541 atagatcgcc aattcggtgg ttttttcggg tgacccctcc catattggca gttttgacaa
    18601 attgaaaccg tacatcatgg atgttcccgg atatttttgc aatatcaatt tggcggaaag
    18661 tgttgcccat tttccaatat cacctgttgc ttcgcctgat aacctgaaac tattgagtgc
    18721 atccagcaag ccatattggc cctgaaccag tagcgaaaaa ggtccgactg acccagaaag
    18781 actcccgtgt agccaaagtt cgttccaatt gtaagcagta ctgtcaagtg ttatgttgta
    18841 acgtttgtag ctgacacccg gctctagtac aaagctcctt ctgtttgacg ctatgcctgt
    18901 ctgtaagccg aaataatttt gaataccctt cacttcagag aaatatctta atcctttagg
    18961 atatgtataa aacggaccat aaaagtcaac attagggtat gtatctgaaa atttgaatgt
    19021 ctgctttaca tatctggact ggtggacaag atagaacaga cccggtttgc ccgggttgac
    19081 caggctgtac aattgcttat aactcagctc atattgtaca tatcgggtat cggcattgag
    19141 cagccttacc ggtattgctt ctcgccgcca atatatttca ttatcaaaca aagtatctgt
    19201 agcaatgccg ccattgtcct gctggttgtt gtaattagag ccatacataa gccatgcttt
    19261 gtaccggttc ttcatgccct gataccaaag ccccaatgtg agacctgtat gcttgatttt
    19321 ctgtcgctgg taatacccga gattcgccat ccttgaaaaa tcgaggtgta gagaaagtgt
    19381 tttgctcaat tctctgccat aagtaccacg gaagatgcca ttaccctgcc cgcccatttg
    19441 tgtaaaaaga accttagtat aaggcctgta acccttgtag aatatagcat tcgcaacatt
    19501 cagcctataa aggtcgaagg catgaaaacc catatcaaat gttatatccg gcacggcatc
    19561 atataccaga aatttgtgtg agctgccaat gttcccgaga ttgccaaaat aaatgccttc
    19621 ctgtcggatg gggtccggct gctggatgcc atctggtata gcataaaggt caacaacacc
    19681 ttctgcctga tcagagaaag cacgaaactg gtaaatcacc gaactgtcag atgtcagagc
    19741 ccgggcgtct ttgtttggtg gaatactatc ctgccccgca aggagaatta caggaaaaat
    19801 gaacaacacc atcaaaagta cccgcaccat attttatact cctgtattag cctgtgggtt
    19861 ccagtcaaga atagccggac tcattcccat gcttgaaaaa ccaccatcat ggaacaagtt
    19921 ctgcatagtg accattctgg taagatcgct gaacatcgta acacaataat tggcacagtc
    19981 tagtgcagat gcattaccca aaggtgacat cttatcggca tattggaaga attctgtaaa
    20041 tcccttcact ccactgcctg ctgttgtcat ggtaggcgac tggctgaggg tattgacacg
    20101 aacatgtttc ttgacaccaa agtgataacc aaaactcctg gcaaatgatt cgagcaaagc
    20161 tttggcttca gccatctcgc tgtaatcggg gaatgtacgt tgggccgcaa tgtaagtcag
    20221 agccagtata gagccccagt cattgagtgc atctttcttg taaagcactt gcatcatttt
    20281 gtgaaatgaa atggctgaca catcgagtga cttctgcatc cactcgtagt tgaggtcggt
    20341 atagtccttt ttctttcgga cgttgagcga cattccgata gaatgaagta caaaatcaat
    20401 tttttgtccg ccaaaagact caatcgactt gtcaaccaac tgttcgaggt cagctagttc
    20461 tgttgcatcg gccggtataa cttccgcatt tagtttttct gcaagtttgt aaatttcacc
    20521 aaaacgcatg gctaccggtg cattggtaag tgtgatgcgg gcaccttctt ctactgcacg
    20581 ttcggctact ttccaggcta ttgactgttc gtcaagtgcc ccgaatatta ttcctgtttt
    20641 tcctttaagc agattgtttg acatatcagt ttattttatg gttgcgtaaa aatacatgtt
    20701 ttgcttcaat tcaggaaatt tccattaatt cccgtgcatt ttcaagtgct gcctttgtag
    20761 gcggatcggt gctgagcatg atggcgatac tggcaattct ttcatcaaca gacagttcgc
    20821 gtacataagt cttggtttgc gtaccaatat cggttttata tacatgataa tgtttgttgg
    20881 ctttggccgc tatctgtggc gaatgcgtga tgacgattac ctggtgcttg tctgaaaggg
    20941 tccggaggat gtttcctact tttaaagata ctgccccact tacacccgaa tctatttcat
    21001 caaaaaccat cgtaggtaat ggaatggagc ttgctaccaa agattgaata ctcaaagcaa
    21061 gcctactgag ttcacctcca cttgctaccg ttcttatttc tgccggcact gatcctttat
    21121 tggccgaaaa cagataactg atctgttcca agccgtgtgg gccaggttgt tcagattcgt
    21181 agagatgtac cttgagtaga gcatttggca tattgagttg ttgcagcatt tcatttactt
    21241 ttgactctag aaccaatgca gcgctctttc ttcctttact tatttctgct cctgttgact
    21301 ttaggtgtgc caagagctca acaatctttt tctctagttt tgccagcatc tctcctacat
    21361 ctccctctcc gttcagctgc agttcgagtt ggtttcgcat tgacatgagc tgatcgctat
    21421 tctggagttt gtgctttttg aggaggcgat atatctcatc cagttttgtc tggaagatat
    21481 tggttttttc cggatcaaat tctgttttgt ctgctagttg ctcaagcgat accgcaatat
    21541 cctctatctc atattgtacc tgctcgagtc tttctgataa gcctccgatt ccatcattga
    21601 atttacttac agcttgaagt agaagattca catcgcgtag ctgttgaaat atactgttgt
    21661 cagattcact gaggtgttgg taagcagcat tcaaattttt tttaatcaat tcggcatttt
    21721 cgaggatagc cagttcgctc tccatcatat tctgtatgcc ttctgtaaga tggagtgcct
    21781 caagttcatc aaacaaaaac tgacggtact cttgctcagc ttcctgtttg tcagaagcac
    21841 tgcgcaattc ttccaaactt tttactgcct gcacatattc cttaaattct gtttcgtatg
    21901 ttttgatt
//
LOCUS       DAOQQY010000018        21387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_735_length_21387_cov_6.516079, whole genome shotgun sequence.
ACCESSION   DAOQQY010000018 DAOQQY010000000
VERSION     DAOQQY010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21387
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_735_length_21387_cov_6.516079"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1763)
                     /gene="rny"
                     /locus_tag="PLE23_02055"
     CDS             complement(114..1763)
                     /gene="rny"
                     /locus_tag="PLE23_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HMX82077.1"
                     /translation="MMNIIIAVIGLAVGFAISTILNRTANRKRIEENNRQADLIVQEA
                     RLTAKRLTDEAEMNAEKAISKSEAANEKIKQKKIQEARERFSQMKQDFDESTAKQKIE
                     LKELELQARAIQNEIKQKQDELKKEKEELESKELEIETIRENLDRQLAIVKRKKEDLD
                     KASETHIKALEQVAKLSEAEAKEQILTAVKAKTENDALVIVKEGIEQANLNANREAKK
                     IVIQTIQRMASEFTIENTVSVFNLESDELKGQIIGREGRNIRALEAATGAEIIVDDTP
                     EAIVISSFDPIRREICRLSLKKLVADGRIHPARIEEVVAKTRKQLDEQIIEIGERTVI
                     DLDIHGLEPYLIKMVGRMRFRSSYGQNLLKHSIETANLCGIMAAELGLTAKQAKMAKR
                     AGLLHDIGKVSEEETELSHAILGMQLCEKHKEHPSIVNAVGAHHDEIEMNNIISPIVQ
                     ACDAISGARPGARREILESYLKRIGELEELAMSYNGVSKAYAMQAGRELRVIVESEKV
                     SDDQSDDLSFKISQRIQDEMQYPGQIKVTVIREKRAVAYAR"
     gene            complement(1845..2144)
                     /locus_tag="PLE23_02060"
     CDS             complement(1845..2144)
                     /locus_tag="PLE23_02060"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMX82078.1"
                     /translation="MTLFKGKVLEIKIAGRSYPVEVQNGEEEILANAMKTVQKELNNF
                     KNAYGTQDNQDLLAMTLFTYTMSMEHQKRAQYTYNNSELLSQILELERMIDMRLA"
     gene            complement(2151..2447)
                     /locus_tag="PLE23_02065"
     CDS             complement(2151..2447)
                     /locus_tag="PLE23_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82079.1"
                     /translation="MQDELHTGIGRLQDKVQLLLKKYDELEQENTELKDQIEKLKSEN
                     LLLTEKITNTTNTARQNQLVMNFDTMPTDNRRVKEEIEAAVTKIEKCIDWLQKN"
     gene            complement(2463..4919)
                     /gene="pheT"
                     /locus_tag="PLE23_02070"
     CDS             complement(2463..4919)
                     /gene="pheT"
                     /locus_tag="PLE23_02070"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMX82080.1"
                     /translation="MKISYNWLSDYLKTSLSPESIGELLTDIGLELEGLETVESIKGG
                     LRDIVIGQVLTCEKHPNADKLSVTTVDAGQGTPLHIVCGAPNVAAGQKVLVALVGAKV
                     YGKDGLSFEINKGNIRGEVSEGMLCAEDELGLGNSHEGLLILPDETKVGTLAAEYFNV
                     TTDYVFEIGLTPNRSDATNHIGVARDLYAALKFRSIDNTASFCTPDVVEDVSIADNKS
                     LHIEVENTAACPRYSGIMIESITVGESPDWLKTRLLAIDQRPINNVVDITNYILHETG
                     QPLHAFDADKIVENTIKVKNLPDGTAFTTLDGIERKLSANDLMICDGDSAPMCMAGVF
                     GGQHSGVSDATKRIFLESACFNASSIRKTSTAHNLRTDAARCFEKGSDPDATVFALKR
                     AIYLFRKYTDAVILGPITDIFPTPIERRKVKVRFHRINSLIGTEISQQNIRTILQLLD
                     MQVVAEDKESLTITIPNNKTDVWREVDVIEEIFRIYGLNEIPTDSRLSFSYNSRQYPD
                     PTTVKSDIAKLLSGIGLNEIMSVSLTQSSYFEKGFNIPREKLVFINNTGNVHLDIMRP
                     TLLVSGIEALSYNINRQQHNQQNNYRFFEFGRKYIKEDDQILEKDQLAIFMVGQRWPE
                     NWNIADKSKLGFGDIKAVVEYILNKTGISGYQETVLTDEHLTAGLMLHRGPQVLARIG
                     KLKATVCKTFDIKQEIWAAVIEWHEILKLTGKQAIHATELNKFPSVRRDLALIIEKNI
                     KFSDIAKIANKAGKPLLKELMLFDVYENAKQIGETKKSYAVSFIFEQTNGTLKDEEVE
                     QLMQKIRKSCEEQLGAFIRH"
     gene            complement(5040..5852)
                     /locus_tag="PLE23_02075"
     CDS             complement(5040..5852)
                     /locus_tag="PLE23_02075"
                     /inference="COORDINATES: protein motif:HMM:NF033782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28 family outer membrane lipoprotein"
                     /protein_id="HMX82081.1"
                     /translation="MKYFFIFISALLTFTACDDRVPILYDAPSKLGDQKLIIEEFTGV
                     QCVNCPQGSDEIASFVAAYPGKVIPVSLHAGYFATPVPGKSVQDFRTPDADFLYEYIG
                     PAPFYPSAVFNRKVFSNNDLIYNQPSWAGLVQQVLESNSKCGLSLETSYNEANRELTV
                     TISGIAKENINDGILAHVMITESNIVEWQKYPNAQGYIADYVHKHVLRGIVSGFTGDK
                     VADNPKAKQNFSKTYKYKLPEKWVVENCEVVGFVSYASKKEIIQAESKEVLN"
     gene            complement(5865..7535)
                     /locus_tag="PLE23_02080"
     CDS             complement(5865..7535)
                     /locus_tag="PLE23_02080"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HMX82082.1"
                     /translation="MQIKYLSILSAFLFCFVLMNNLMAQEESKGGKFFGSLQTNGNFF
                     MKDSAIGASNTPQYDNKKFGAESWLNLNYTAKDLDIGIRFDLFNNSNIVNPTGSYTDQ
                     GIGAWWIRKRINKLELSGGYLYDQIGSGIIYRAYEDRPLAIDNALYGVKLQYDITPDL
                     RIKTFAGKVKTFFTSSGPLIKGASIDGFVAGKEGSGWSIAPGIGIVNQTFDDETIQKM
                     VATVKNYKPEDGIKLYHNTYAGSVFNTLTYKNISLYTEAAYKLHDVYRDPDALVTLSN
                     GTTTLGKFRDNPGSVLYGALTWSMDKFGVTVEYKRTENFRYRIDPFASGNFGAINFLP
                     PMSRPSTYRLTAFYSAVTQELSEQAFQAEVNYKVNKKLNLIFNTSYIDDLSGKLLYRE
                     LYGEAQLKLHKKWIINAGIQRQIYNIATYLVKPGEENVKTITPFVDVLYKFTPKKTVR
                     FECQYLNTEQDRGSWVQALIEVGIAPHWILTVSDMYNLSNDQKILDKHPELQSKTHYL
                     TAGGVYTYKSSRFSLNYVKQVAGIVCSGGVCRYEPAFSGIRVNVLSHF"
     gene            7676..8167
                     /locus_tag="PLE23_02085"
     CDS             7676..8167
                     /locus_tag="PLE23_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMX82083.1"
                     /translation="MKKNLLLFLSLIFFGSMFAQNALPNIDIKNLEGKTIKIKDAVEP
                     GKVTILGFWATWCSPCKKELDAIGENWDEWKEKYNCELLAITVDDQRSLGKVKPMVKE
                     KGWTFPVYSDANQQLLKGLSGQSVPFTVVLDKAGNIAFIHNGYTPGDETELEAKIAEL
                     SAK"
     gene            8434..9987
                     /locus_tag="PLE23_02090"
     CDS             8434..9987
                     /locus_tag="PLE23_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMX82084.1"
                     /translation="MSQVVGNNDDLAFYAEDGVHPEVAKIQNFSGKYPKQLWFLFFSE
                     MWERFCFYGMRGMLTFFMVYELGKAKDVANLQYGATQAWVYAFTFIGGLFADQILGFK
                     KSLFWGGLLMIAGSIILAMDPHKFFFLGIAALVIGTGFFKPNISSMVGQLYRADDHRR
                     DAGFSLFYSGINIGAFLGGILMIGIGKGGILGGVIPEDNRWQVAFGLVAVVMCISLVT
                     FMMYKHQLGPIGDMPGNMSDRKNRWKEWMVYIGALACIPVIQIMVSNTHYTDIFMYII
                     GPFALIYLIYEMTKLTWKDNSKLIAALVFILFSVVFWAIFEQAGGSLAIYAPEHLKSN
                     LLGLPVDPNMVNNSANALFIIFFAPVAGLVWLWLHKRKMEPNGVVKFALGFLFLGIGF
                     YLFFTNKLFADSNAMTSLNFFAFAYIIITFGELCLSPIGLSLMTKLSTQRLQGMMMGM
                     WFLASAYGQYVAGLFGASISPAENADRLEKLSVYSEGYKQFGIYAIVAGIILLALSPF
                     IKKLMREVS"
     gene            10148..11704
                     /locus_tag="PLE23_02095"
     CDS             10148..11704
                     /locus_tag="PLE23_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMX82085.1"
                     /translation="MNAQSTQSGHPKGLYLLFFTEMWERFSYYGMRAIFILFLTKALF
                     LSSKDGSNIYGSFTGLVYLTPLLGGYLCDKFLGNRRSIFIGGVLMAIGQFMLFLCGNM
                     IANDTTAATLPPMAMTLLWGGLTVLIVGNGFFKPNISTMVGQLYEKGDRRVDSAFTIF
                     YMGINLGAFFSPFVCGGLGDTGSIADFRWGFLAACIGMIISVFSFELLKNKYLTGPQG
                     EQIGMPVQKLDTKNLGIVIGSFIVIFFLLSFKSLFNSDLDVIGYLIYGAMVVVPLVII
                     TDKSLKSEEKSRIAVIFILAFFVIFFWSAFEQAGASLTLFADAQTDRNIGGWTMPASW
                     FQSVNPLGIIILAPIFSYFWGMLHKKNLEPASPMKMAWGLALLCLGYIVIAMAVKGVD
                     SSVKISMWWLIALYLLHTMGELCLSPIGLSMVSKLAPLRFSSLLMGTWFLANAAANKL
                     AGTLSALIPPTAEDIAKAGGTAKYPALLGFQITNLYEFFMVFIVMSGTAAIILFLLSG
                     VLRKMMRGIK"
     gene            complement(11898..14075)
                     /locus_tag="PLE23_02100"
     CDS             complement(11898..14075)
                     /locus_tag="PLE23_02100"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMX82086.1"
                     /translation="MKKSALLLLFVLSMGSLCSQTLKTFDLEDIFKKGTFRTQNLPGF
                     AYMKDGKSYIINKGDRLEIYSVADGKQTGTFIDKAQLTAISGDNNLYIEEYFFNDDES
                     KLLIYTQSESIYRHSSKGKTFVFDKKTGTGSVIFDNKLVSNPTFSPDGQKVAFTFENN
                     LYYTDLTTGKTIQVTTDGKWNYIINGMCDWVYEEEFSFTKAFEWSPDSRKIAFLRFDE
                     TNVKEFTIEYFKDEVYPEPYSFKYPKVGEDNSTLEIKIFNLDNGKTIAIDQVRDKEIY
                     YPRIKWTNDPGKLCVFKMNRHQNKLDLLLADATSGKTSVLMTEENKYYIEINDYLTFL
                     KDGKRFIWVSEKDGFNRAFLYDMTGKAVNAITQANADLSEIYGVDEKNNILYYQIIAP
                     TPMQREVYKVSLNGKNAVKLSEKTGTNSAQFSPTFEYVTMQYSSVNTPPTWTLYDKNM
                     KVIRLLEDNRKAVDKIVQYKFGKTEFFNFTTSEGVNLNAYMIKPTDFDPNRKYPVFMF
                     LYGGPGSQQVLDQWGRAQTWWFRMIAERGYIVACVDNRGTGGRGEEFRKMTYMKLGHY
                     ETIDQIEAAKYLGSLPYVDKSRIGIFGWSYGGYMSSLCLLKGNDVFKAALAVAPVTNW
                     KWYDSIYTERYMRTEKENPDGYKDNSPVYFADRLKGNYFLAHGMADDNVHFQNSAEMN
                     RALIKAGKSFDFHMYPNSNHGIYSEGATMHLYNEMTKFILEKL"
     gene            14462..14785
                     /locus_tag="PLE23_02105"
     CDS             14462..14785
                     /locus_tag="PLE23_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82087.1"
                     /translation="MKIFAFLFSFYILALSVVPCTDQAGCGFQTEQTDNHSKEEGHHH
                     DSESKDHCTPFCVCTCCSQAFNHNFYKSAITERKEGVTHECPAYTSSFISETFFQIWQ
                     PPKIS"
     gene            14882..19225
                     /locus_tag="PLE23_02110"
     CDS             14882..19225
                     /locus_tag="PLE23_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMX82088.1"
                     /translation="MLEKIIAFSIKNKFLVILMTLALIMWGMISATKLPIDAVPDITN
                     NQVQIITLCPTLASQEVEQLVTYPIEQTVSNLPDVEELRSISRFGLSVITVVFDESVD
                     IYFARQLISEKLKEAAERIPKGIGQPEMAPVSTGLGEIYQYIIHPKKGSEHKYSPADL
                     RTMQDWIVARQLYGTPGIAEVNSFGGQLKQYEVAVNPDRLLAMDVTIPEIFTALEKNN
                     ENTGGAYIDKKPNAYFIRGVGLIGSFDDIKNIVVKSNPNGIPILIKDVADVRFGGAVR
                     YGALTYNGEKDAVGGLIMMLKGANSAEVVNRIKEKLPTIQKSLPADVTIEPFLDRTDL
                     VNRAISTVEKNLIEGALIVIFVLVLFLGNFRAGLIVASAIPLSMLFALGMMNVFGISA
                     NLMSLGAIDFGLIVDGAVIIVEATMHHLGLRKITGRLTQDEMDKEVFESASKIRNSAA
                     FGEIIILIVYIPILTLIGTEGKMFRPMAQTVGLAIAGALILSLTYIPMMCSLFLSKNI
                     SHKETFSDRMMRFFQGIYTPLLEKAIRFKNILVAATVLIFAITVFIFSRMGGEFIPTL
                     QEGDFAFHCILPQGTSLSQSLETSMQASRILREFDEVRMVVGKTGSAEVPTDPMPPEA
                     TDMMVILKPPKEWKRNISYNALAEEMMHKLESLPGVFFESSQPIQMRFNELMTGIKQD
                     VAVKVFGENLDTLLLYANKIAPIISSVKGTTEPSVERIAGLPQIVIRYNRSQMANYGI
                     NIQDINRIVSTAFAGGSAGVVYENERKFDLVVRLDSTNRSKIDDVSHLYIPAANGTQI
                     PLSQVAEIKLELGPVQISREDGKRRIVVGFNVKDRDVESVVTEIREQLDKNLKLSEGY
                     YYTFGGTFESLQAASKRLMIAVPVALALIFLLLYLTFGSASQALLIFTAIPMSAIGGV
                     FALLARGMPFSISAGIGFIALFGVAVLNGIVLIATFNQLKKDGMENITERIIEGTKIR
                     LRPVLMTATVASLGFLPMALSHGAGAEVQKPLATVVIGGLLTATFLTLFVLPLLYMIF
                     FTGKRISIKPLAILPFLILLADNVDAQKVLTEEEVVSIALKNNSGIQSQDLGIMASSE
                     MRKTVFELPATAFNVQYGQFNSINNDIGFQVDQSIQMPAYFSAKKQLYNAQVQADQMK
                     KDISVNELRSKVRSLYAELRYLSFARELLLSVDSLYNDFIKVADIRYRTGETNLLEKM
                     TAETRRGQFSLMLQQNAAEYINTYQSLKALMYTPEDFAPIAPKDYTPASLTIAADTSM
                     LESNPALRYLYQQTVVAEKDKKFVKTAMLPELSVGYFNQSITGAQNIDGNEVFFGRDK
                     RFQGINVGVSVPLIFSSNAAKLRSLTYRGLALQKEAEQSKALLQSQLLNEKRTYQRNL
                     ERLEFFTKTALPNADTMINTATSSFRHGEIGYIEYLQALQSAAEVKLDYLDTIRQLNQ
                     NILNINYLLNQ"
     gene            19236..20558
                     /locus_tag="PLE23_02115"
     CDS             19236..20558
                     /locus_tag="PLE23_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMX82089.1"
                     /translation="MNRYILIIIVSLISLLTSCHQHTEGDGHSHGTEDAHAGHSHENT
                     EDLTTTSLTDEQIKSIKLEYGKIEKKQLTASLKANGLLRVPNNNRATVSSLFSGVIKS
                     ILVQTGKNVTKGQVIATLTNTSLINMQEEYLSITAKLELAQLEYERQQAMKENNASAL
                     KTFQQAATELKTLKARKASLKTQLNMAGINTKTLSSENIQSVLSIKSPISGAISTLNI
                     NIGSFVETNTAIAEIVDNSQLHVDLYVYEKDLYKLRNGQTIHFTLTNNPGKEYDADIY
                     SISNTFEPNTKAVSVHATVKGNKQGLIDGMSITALVSLEDATVEAVPVSAIVNHEGQD
                     YIFIVTNGPAANQDEHDHEGHDHEGHNHDDHNHEGHNHDEDHKETKTTASPTGTFFER
                     IPIKRGTTDVGYSEITLLKDVPKDVKIVTNGAFFIMAKMTNQGEAHEH"
     gene            20679..>21387
                     /locus_tag="PLE23_02120"
     CDS             20679..>21387
                     /locus_tag="PLE23_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82090.1"
                     /translation="MKLPFYDRKFLLLIAAIVIVITLEILSIAGIHIPMPYAPFIFAS
                     FIIGIGYEVLWEGIRAIGKLNFSSINLLMTIAVMAAFYLGEYPEAAVVIVLYVLGERL
                     EDIGIENSKSAMDELISRAPKKAFVKLLNKEVFIDDIQIGTIIQVKPGEMIPLDGKII
                     SGETSIDEAAITGEPIPKDKHAGDNIFAGTLNKNGFIEIETTRLSADTTFSRIVRLTF
                     EASANKSDTQKFIQMFSR"
BASE COUNT         6031 a         4374 c         4299 g         6683 t
ORIGIN      
        1 ggtagcgagc ctacacgcca ataacatatt tcaagcaaca taaaaaagag acccctgaat
       61 tttcagaggt ctctttttta gtattttggc attaaacccg gttttgtggg ttattatctc
      121 gcataagcaa ctgcacgctt ttctctgata accgtcactt ttatctgccc gggatattgc
      181 atttcgtctt ggatgcgctg cgaaatcttg aatgacaaat catctgactg atcatcggat
      241 actttttcac tctctacaat aacacgcaat tcgcgacccg cctgcatagc gtaagccttt
      301 gagacaccat tataagacat agccagttct tcgagttcac caatgcgctt gaggtagctt
      361 tcgaggattt ctcttcgtgc tcccggtcgt gcacccgata tagcatcaca agcctgtaca
      421 ataggagaaa tgatgttgtt catttctatt tcgtcgtggt gggcacctac ggcatttacg
      481 atcgacgggt gttctttgtg cttttcgcac agctgcatac caaggatagc gtgcgatagt
      541 tcagtttctt cttcagacac tttgcctatg tcatgcaata gtcctgcacg tttagccatt
      601 ttggcctgtt tggcagtcaa accgagttcg gcagccatta taccgcacag attagccgtt
      661 tcgatggagt gtttgaggag gttctgacca tacgatgaac gaaaacgcat acgtcctacc
      721 attttgatga ggtagggctc tagtccatga atgtcaaggt caataaccgt tcgttcaccg
      781 atttcgatga tttgctcatc gagttgtttc cttgtttttg ctaccacttc ttctattctg
      841 gcagggtgga tacgtccgtc tgccactagc tttttaagag aaagtctgca gatttctctc
      901 cttatcgggt caaagctgga aataacgatt gcttcaggtg tgtcgtcaac gatgatttcg
      961 gcaccggtag ctgcttcgag tgcacggatg tttctgccct cacgacctat gatttggcct
     1021 tttagttcat ccgattcaag gttaaaaaca gaaacagtat tttcaatcgt aaattctgat
     1081 gccatccttt ggatggtctg gatgacgatt ttctttgctt ctctgttagc attcaggttt
     1141 gcttgttcga taccttcctt gacgataacg agtgcgtcgt tttctgtttt agcctttact
     1201 gctgttaata tttgttcctt ggcctccgct tcactcagct tggcaacttg ttcgagcgct
     1261 ttgatgtgtg tttcacttgc cttgtcgagg tcttctttct tgcgtttcac gatggcaagt
     1321 tgcctgtcaa ggttttcgcg tatggtttct atctccaatt ctttagattc gagttcttct
     1381 ttttcctttt ttagctcatc ctgtttttgt tttatttcgt tttgtatagc acgagcttgt
     1441 agttctagct ccttgagttc gattttttgt ttagcagttg actcgtcaaa atcctgcttc
     1501 atttgagaaa aacgctcacg tgcctcctga attttctttt gtttgatttt ttcattggca
     1561 gcttcagatt tcgagatggc tttctcggca ttcatttctg cctcgtcggt caatcttttg
     1621 gcggtaagtc ttgcttcctg gacaatcaaa tctgcttggc ggttattctc ttctatccgc
     1681 ttccggttgg ctgttcggtt caatatagtg gatattgcaa atcccacagc taaaccgatg
     1741 acagctatta ttatattcat cattgtatta agaactttat gttaattaaa aattattgtc
     1801 aaatattttc tgacaaaaac aactaacaca aagaagaaat ttacttaggc caaacgcatg
     1861 tctatcatac gttcgagttc aagtatctgc gacagcagtt ccgaattgtt ataagtgtat
     1921 tgagctcttt tctgatgctc catcgacatt gtataagtaa acaatgtcat cgccagaaga
     1981 tcctgattat cttgtgtgcc gtaggcattt ttaaaattat tcagttcttt ttgtacggtt
     2041 ttcatggcat ttgctaaaat ttcctcctct ccgttctgta cttccacggg gtatgacctt
     2101 cctgctattt ttatctccag tacttttcct ttaaataagg tcattatagt ttaatttttt
     2161 tgcaaccaat caatacattt ttcaattttc gtcactgcag cttctatttc ttcttttacc
     2221 cgtctgttat ccgttggcat ggtatcaaag ttcatcacta actgattttg ccgggcagta
     2281 ttagttgtgt ttgttatttt ttcggttaat aataagtttt cactcttcaa tttttcaatt
     2341 tggtctttga gttcagtatt ttcttgttcc aattcgtcgt acttcttcag caatagctga
     2401 actttatcct gcaatctgcc aattccggta tgaagttcgt cctgcatgaa gtatgtttgt
     2461 atttaatgtc tgatgaatgc gcctaactgt tcttcacagg attttcttat tttctgcatg
     2521 agttgttcga cttcttcatc cttaagtgta ccatttgttt gctcaaagat aaaacttacc
     2581 gcgtaagatt ttttggtttc gccaatttgt tttgcatttt cataaacatc aaacaacatc
     2641 agctctttta acaacggttt gcctgccttg ttcgcaattt tcgcaatatc cgaaaatttt
     2701 atattttttt caattattaa tgctaaatct cttcgcactg aaggaaattt attcaattct
     2761 gttgcatgaa tagcttgttt tcctgtcaat ttcaatattt catgccattc gatgactgcc
     2821 gcccatattt cctgctttat atcaaatgtt ttacaaactg tggcctttaa tttcccgatt
     2881 ctggctagta cttgtggccc tctgtgcaac atcagtcctg ctgtgagatg ctcatctgta
     2941 agtactgttt cttgataacc gcttattccg gttttgttca gtatatattc taccacagcc
     3001 ttgatatcac caaaaccaag ttttgatttg tcagcaatat tccagttttc aggccaacgc
     3061 tgtccgacca taaatatcgc aagttgatct ttctcaagta tctgatcatc ttctttgata
     3121 tacttacggc cgaattcaaa aaaccggtaa ttgttctgct gattgtgttg ctgtctgttg
     3181 atattgtacg acaatgcctc aatacctgat acaagcaaag taggtcgcat gatatcaagg
     3241 tgtacattcc cggtgttatt gataaatacc aatttttctc taggaatgtt gaaccctttt
     3301 tcaaaatatg aagattgtgt gagcgatacc gacattattt cattcagacc tattcctgaa
     3361 agcaacttag ctatatcgga ctttacagtt gtagggtccg ggtattgtct tgaattatat
     3421 gaaaaactta gcctgctgtc ggttggtatc tcattaagcc cgtatatcct gaatatttct
     3481 tcaataacgt ccacctctcg ccatacatct gtcttgttgt tgggtattgt tatggtcagg
     3541 ctttctttat cttctgcaac tacctgcata tcaagtagtt gaagaatggt tctgatgttt
     3601 tgctgcgata tttctgtgcc tatgagacta ttgatacgat gaaatcttac ttttaccttc
     3661 cgcctttcga ttggagtagg aaaaatatct gtaatcggtc ccaaaatgac agcgtcggtg
     3721 tattttctga ataaatatat ggctcgtttc aatgcaaaaa ctgtagcatc cgggtcactt
     3781 cctttttcga aacacctggc tgcatctgtt ctaagattgt gtgcagtact tgttttgcgt
     3841 attgaagatg cattaaaaca ggctgattca agaaatatac gctttgtggc atcgctaaca
     3901 cctgaatgct gaccaccaaa aacaccggcc atacacatcg gggcagaatc accgtcacaa
     3961 atcatcaggt cattcgcaga taattttctt tctatgccgt caagtgtggt gaaagctgta
     4021 ccatctggta gattttttac tttaatggta ttttcgacta ttttatcagc atcaaatgca
     4081 tgaagtggct gtccggtctc atgcaggata taattggtaa tatctaccac attattgata
     4141 ggcctctggt ctatggccaa caacctggtt ttcaaccaat cgggtgattc gccaactgta
     4201 atactttcaa tcataatacc cgaatacctt gggcatgctg cagtattttc tacttcgata
     4261 tgtaagctct tattgtcggc tatgctgaca tcttcaacaa catcaggtgt acagaatgag
     4321 gcggtattgt caatggatct gaattttaaa gctgcgtaca aatcgcgtgc tacgcctata
     4381 tggttggtag cgtcggaccg attaggagtc agacctattt cgaatacata atctgtcgtc
     4441 acattgaagt attctgctgc aagtgttcct actttggttt cgtcaggcaa tatgaggaga
     4501 ccttcatggc tattgccgag tccgagttcg tcttctgcac ataacattcc ttctgatact
     4561 tcgcctcgga tgtttccttt gttaatttca aatgacagcc cgtctttacc atacactttt
     4621 gccccaacca atgctacaag tactttctga ccggcagcta cattgggtgc gccacacact
     4681 atatgcagcg gggtaccttg acctgcatca acagttgtga cagatagttt gtcggcattc
     4741 gggtgttttt cgcaagtaag cacctgaccg atgacgatat cccttagacc gccttttatg
     4801 ctttccaccg tctccaagcc ttcgagctcg agcccaatat cagtaagaag ttcaccgatg
     4861 gattctggtg agagagatgt tttcagataa tcgcttagcc agttataaga aattttcatt
     4921 taattgtcgt attcgttaat ggccgcaaaa atatgaaata tccatttttc ggcacttaca
     4981 agagtgtcaa ttagttctac gttttttcaa cagacatgtt ttaaaatcca atgctgttat
     5041 taattcagca cttctttaga ttctgcctga atgatttctt tctttgaagc ataactaaca
     5101 aatcctacaa cttcacagtt ttctacaacc catttttcgg gcagcttata tttgtaagtt
     5161 ttgctaaaat tttgttttgc ctttggatta tcggctactt tatcacccgt aaaacctgag
     5221 acaatgccac gtagcacatg tttatggaca taatctgcaa tataaccctg tgcattgggg
     5281 tatttctgcc attcgactat gttagattcg gtaatcatca cgtgtgcaag aattccgtca
     5341 ttgatgtttt ctttggcaat acctgagatg gttactgtga gttcgcggtt agcttcatta
     5401 tagcttgtct ccagactgag accacatttc gaattgcttt ccaacacttg ctgtaccagt
     5461 cctgcccaac ttggctggtt gtagatcaag tcattgtttg aaaaaacctt acgattaaat
     5521 actgctgaag gatagaacgg tgctggcccg atatactcgt acagaaagtc agcatcaggt
     5581 gttctgaaat cctgtacaga tttgcccggt accggagtag caaaatatcc ggcatgcaat
     5641 gatactggta tcaccttgcc aggataagcg gcaacaaaac tggctatttc gtcagaaccc
     5701 tgagggcaat taacacattg tacgcctgta aattcttcaa taatcagttt ttgatctcca
     5761 agtttgcttg gtgcgtcata taagataggc acacggtcat cacatgctgt aaatgtcagt
     5821 aatgcagaga tgaaaataaa aaaatatttc atgataatat ttgattagaa atgtgataaa
     5881 acgtttactc tgataccgct gaacgctggt tcataacgac atacaccacc agaacaaacg
     5941 attcctgcca cttgtttaac ataattaagc gaaaaacgac tcgatttgta tgtataaacg
     6001 ccacctgcgg tcagatagtg cgttttgctt tgcagttcgg ggtgtttgtc caatattttc
     6061 tgatcatttg acaaattgta catgtcagat actgtcagaa tccaatgtgg tgcaatacct
     6121 acctctatca gagcctgtac ccaactacct ctatcttgct cggtgttgag atattggcat
     6181 tcgaagcgaa cggtcttttt gggtgtaaat ttgtaaagga catctacgaa tggtgttatg
     6241 gtttttacat tctcttcgcc gggttttaca aggtaagtgg ctatgttgta gatctgtctt
     6301 tgaatacctg cgttaataat ccatttttta tgtagtttca attgtgcctc tccgtagagt
     6361 tcgcggtata gaagttttcc tgaaaggtcg tctatataac ttgtgttgaa tatcagattg
     6421 agttttttgt tgaccttgta gtttacttca gcctggaatg cctgctcaga caactcttgg
     6481 gtgactgcac tgtagaatgc tgtaagtctg taggtacttg gtcttgacat aggaggcagg
     6541 aaattgatgg ctccaaaatt gccggaggca aaagggtcaa tcctatacct gaaattttcg
     6601 gtacgcttat attcaaccgt tacaccgaat ttgtccattg accacgtaag ggcgccatac
     6661 agaaccgagc ctggattgtc acgaaacttt ccaagggtag tggtcccgtt cgacagtgta
     6721 acaagcgcat ccgggtcgcg gtacacatcg tgcagtttgt aggcagcttc ggtatagagt
     6781 gaaatattct tgtaagtcaa tgtattaaat acacttcctg cgtaggtatt gtggtatagt
     6841 tttattccgt cttcgggctt atagtttttt acagttgcta ccattttctg gatggtttca
     6901 tcatcaaagg tctggttgac tatgcctata cccggagcaa ttgaccagcc tgagccttct
     6961 ttacctgcta cgaatccatc aatacttgca ccttttatca gagggccgga cgaagtaaaa
     7021 aacgtcttga ccttaccggc aaatgttttg atgcgtaggt ctggtgtaat atcatattgt
     7081 agttttacac catacagtgc attatctata gcaagtggcc tgtcttcgta agcacggtaa
     7141 atgataccac tacctatctg atcgtaaaga taaccacccg ataattcgag cttgttgatt
     7201 cgttttctga tccaccatgc acctataccc tggtcagtat aagaacctgt tgggttaaca
     7261 atatttgaat tattaaacaa atcgaacctt ataccaatgt cgagatcctt tgctgtataa
     7321 ttaagattga gccaagattc agctccgaat ttcttattgt catattgcgg tgtattggag
     7381 gctcctattg ccgaatcttt cataaaaaaa ttgccgttgg tctgaagtga tccgaaaaac
     7441 tttcctcctt ttgattcttc ctgtgccatt aaattattca tcaatacaaa acagaacaaa
     7501 aaggccgaaa gaatggataa atacttgatt tgcatgttaa tttttagatt acgtttaatt
     7561 tcagagtgca atttacttaa aatatcgtta aatatgcttt gcccgtatcc aaaaaattgg
     7621 caaatgataa aaatattcat ttaactttac cctgaatatt aaaaaaatcg cctctatgaa
     7681 aaaaaacctc ttgctctttc tttctttgat tttctttggt tcaatgtttg cacaaaatgc
     7741 tttaccaaat attgatatca agaatctcga aggaaaaact attaaaataa aagatgccgt
     7801 cgaacccgga aaagttacca ttttgggatt ttgggctact tggtgttcgc catgtaaaaa
     7861 agaattggat gccatcggtg aaaactggga cgaatggaaa gaaaaataca attgcgaact
     7921 gcttgctata acagttgacg atcaacgttc gctcggcaaa gtaaagccta tggtcaaaga
     7981 gaaaggctgg accttccctg tttattctga tgccaaccag caattgctca aaggtctgag
     8041 cggtcagagt gtaccattta cagtagtact tgataaagcg ggtaatatcg cattcattca
     8101 caacggttat acaccaggtg atgaaacaga actcgaagca aagattgcag aactgtctgc
     8161 caaataaacc tattcatcta cattgaataa gtaaagagcc gtgaagaaag tacttcgtgg
     8221 ctctttttgt tttcaataac cacctattgt aagtcggcat tatatttggg ctgataaaat
     8281 atttgctgaa caaatgttga ttacataata aattgcatta ctttcgctca gtaaaatcta
     8341 aaggtactgc catcacatat tagttagtaa taatggcaat aatctactta aatgtttaat
     8401 gattttttta tttaaatact gaaaaataag cacatgagcc aagtagtcgg aaataatgat
     8461 gatttagcat tttatgctga agatggtgtc catccggaag tagcaaagat tcagaacttc
     8521 agtgggaaat acccgaagca gttgtggttt ctttttttta gtgaaatgtg ggagcggttt
     8581 tgcttctacg gtatgcgtgg gatgctcact ttttttatgg tttatgaatt gggtaaagcc
     8641 aaggatgtgg ccaacctgca atacggtgct acacaagcat gggtttatgc ctttacattc
     8701 ataggaggct tatttgccga tcagattctt ggttttaaaa aatccttgtt ctggggtggc
     8761 ttgctcatga tagccggcag catcattctt gccatggatc cgcacaagtt tttcttcctt
     8821 ggtattgcag cactggtaat cggtacagga tttttcaaac ccaacatatc atcaatggta
     8881 ggacagttgt acagagcaga tgaccaccgt cgtgatgcag gtttttcgtt attttattcg
     8941 ggtatcaata tcggggcttt ccttggtggt attttgatga ttggcatagg taaaggcggc
     9001 attttgggcg gtgtcattcc ggaagataat cgctggcagg tagcattcgg gctggtagcc
     9061 gtggttatgt gtataagtct ggttaccttc atgatgtata aacatcagct cggcccaata
     9121 ggcgatatgc ctggcaatat gagtgaccgt aaaaacagat ggaaagagtg gatggtttac
     9181 attggagcgc ttgcctgcat tccagtaatc cagataatgg tcagcaacac acattataca
     9241 gatatattta tgtatatcat aggccctttt gctctcattt atctaattta tgaaatgacc
     9301 aaactcacat ggaaagataa ttcaaagctg atagcagcac tagtattcat tcttttctct
     9361 gtagttttct gggcaatatt cgagcaggcc ggcggatcac tcgccatcta tgcaccggag
     9421 cacctcaaaa gcaatctcct tggtttgccg gttgacccaa atatggttaa taattcggcc
     9481 aatgcattgt tcataatatt ttttgcacct gtagcaggct tggtctggtt gtggttgcac
     9541 aaaagaaaga tggaacctaa tggtgtggtc aaatttgcat taggattttt attcctcggc
     9601 ataggatttt atttgttttt tacgaacaaa ctttttgccg acagcaatgc catgacttca
     9661 cttaactttt ttgcctttgc ttatattatc attacatttg gagaactatg cctatcaccc
     9721 attggcttat cattaatgac caagttgtcc acccagcgct tgcaagggat gatgatgggc
     9781 atgtggttct tggcgagtgc ctacggccaa tacgtcgccg ggctgttcgg agcttctatt
     9841 tcaccggcag agaatgcaga ccgactcgaa aagctatctg tgtatagtga aggttacaaa
     9901 caattcggca tctatgcaat tgtagccggc attatacttc tcgccctttc accttttatt
     9961 aaaaaattga tgcgggaagt aagctgattt acaaaatagc agatgtctgg tggtgtaaat
    10021 atcagtgtct cacaagataa ggaattgata aaatttggat tttcgataaa tcagattaat
    10081 tttggcaatt gaaaatccgg tgaaatggat acgaaaacgt ttttctaacc aaacaaaaat
    10141 gcggcatatg aatgcacaat ctacacagag tggccatccg aagggcttat atctgttatt
    10201 ttttactgaa atgtgggaac gtttcagtta ctatggcatg cgcgccatat tcatactttt
    10261 cctgaccaaa gccttattcc tgtcatccaa ggatggttcc aatatctatg gaagctttac
    10321 cggtctggtt tatctgacac ctctgttggg tggttatttg tgcgataaat ttttaggtaa
    10381 cagaaggagt atttttatcg gtggtgtgct gatggccatt ggccagttta tgttgtttct
    10441 gtgcggaaat atgattgcta acgacacgac cgcagcaaca ctgccaccca tggccatgac
    10501 attgctttgg ggcgggttga cagtactcat cgtgggcaac gggtttttta aaccaaatat
    10561 ttctaccatg gtaggccagc tttatgaaaa aggcgacaga agagtggata gtgctttcac
    10621 catattctat atgggtatta accttggagc attcttttca ccattcgtgt gtggtggtct
    10681 gggcgacaca ggtagtattg cagattttag atggggtttc ctggctgcct gtatcggtat
    10741 gataataagt gtcttttcgt ttgaattact taaaaataaa tacctgacag gccctcaagg
    10801 tgaacaaata ggaatgcctg ttcaaaaact tgatactaaa aatcttggaa tcgtcattgg
    10861 ttcttttatc gttattttct tcttattgag tttcaaaagt ctttttaaca gtgacctgga
    10921 tgtcattgga taccttatat atggtgcaat ggttgtcgtt cctttggtta ttattactga
    10981 caaatcactc aaatctgaag aaaaatcaag aatcgcagtt atttttattt tagcattttt
    11041 tgttatattc ttctggagtg cttttgagca ggcaggtgct tcacttactc tctttgcaga
    11101 tgctcagaca gacagaaata ttggaggctg gaccatgccc gcatcttggt ttcagtccgt
    11161 aaacccttta ggaatcatta ttcttgcccc gattttttct tatttctggg gcatgctcca
    11221 taagaaaaat ctggaaccag cctcgcctat gaaaatggct tggggtttgg cgttgctgtg
    11281 cctgggatat atagttattg ctatggcagt aaaaggtgtg gacagttctg tcaaaataag
    11341 catgtggtgg ctaatagcac tatacctgct ccatacaatg ggtgagcttt gcctgtcacc
    11401 aatcggcctt tcaatggtgt ctaagttagc tccactgaga ttctcctcat tactgatggg
    11461 cacgtggttc ctggccaatg cagccgccaa caaactggcc ggtaccttga gtgcccttat
    11521 accacctact gctgaagata tagcaaaagc aggcggaaca gccaagtacc ctgccttgtt
    11581 aggattccag atcaccaatc tgtacgaatt ttttatggta ttcatcgtta tgagtggaac
    11641 ggcagcaata atcttgttcc tgctaagtgg tgtactacga aaaatgatgc gaggaatcaa
    11701 ataatcagat tccttcccta ctaatgtaaa gagcgaactc cgaaatgata caggagttcg
    11761 ctctttttac ttgttggcat tgcagtcatg aaaccaaatg cggatataag cttcaaaaat
    11821 ttattatcga aatgatttca attcttcgat ttgttctgat tgtaacatta gaatgtaggc
    11881 cgccctattg acgtacttta caatttttcc agtataaact ttgtcatttc attgtacaag
    11941 tgcatcgtag caccttcgct gtatattcca tgattgctgt ttggatacat gtgaaaatcg
    12001 aatgattttc ctgccttgat aagcgcgcga ttcatttcgg cactgttctg aaagtgcacg
    12061 ttatcgtctg ccatgccatg tgccaggaag tagttacctt tgagcctgtc ggcaaaatat
    12121 accggactat tgtccttata tccgtctgga ttctcctttt cggtacgcat gtatctttca
    12181 gtatagatag agtcatacca tttccagttg gtgacaggtg ctactgccag tgctgccttg
    12241 aatacgtcat tgcctttgag cagacataat gaagacatat atccgccata agaccagccg
    12301 aatattccga tccgggattt gtccacgtat ggaagtgagc cgagatactt ggctgcttct
    12361 atctgatcaa ttgtttcgta atggcccagt ttcatgtagg tcatcttgcg gaattcttca
    12421 cctcgcccac ctgtaccgcg attatcaaca caggctacta tataaccacg ctcagctatc
    12481 atcctgaacc accatgtctg agcccgcccc cattggtcaa gtacctgctg gctgcccggc
    12541 ccaccatata gaaacataaa tacaggatat ttccgatttg ggtcgaagtc agtcggctta
    12601 atcatataag cattgagatt tacaccttca cttgtagtaa agttgaagaa ttccgttttt
    12661 ccgaatttgt attgcacaat tttatcaact gccttgcgat tatcttccag taacctaatt
    12721 actttcatgt tcttgtcata taatgtccag gtaggtggag tgttgacgga ggaatactgc
    12781 atggtcacat actcaaaagt aggtgaaaac tgtgctgagt tagtaccggt tttttcagat
    12841 aacttaactg catttttacc attaaggctt accttataaa cttctctctg cataggagta
    12901 ggcgcaataa tctggtaata aagtatattg ttcttttcat ccactccgta tatctcagac
    12961 aaatcagcat tggcctgtgt gatggcgttt actgctttcc ctgtcatatc gtatagaaat
    13021 gcacggttaa aaccatcctt ttcacttacc cagataaatc gtttgccgtc ttttaagaaa
    13081 gtaagataat cgttaatctc aatatagtat ttgttctcct ccgtcatgag tacagaagtt
    13141 tttcctgaag tggcatcggc aagcaacagg tcgagcttgt tctgatgccg gttcatttta
    13201 aaaacacaaa gcttgcccgg gtcatttgtc catttaatac gtgggtaata aatttcctta
    13261 tcccgcactt ggtcgatagc tattgttttt ccattgtcaa gattgaagat ttttatttca
    13321 agcgttgaat tatcttcacc cactttggga tacttgaatg aataaggctc aggatatact
    13381 tcatccttga aatattcgat ggtaaattcc tttacatttg tttcgtcaaa gcgcaggaaa
    13441 gctatttttc ggctgtcggg cgaccattcg aaggctttgg taaaactaaa ttcttcttca
    13501 taaacccagt cgcacattcc gttgatgata tagttccatt tgccatcggt agtaacttgt
    13561 atggttttgc cggtggtaag gtcggtgtag taaaggttat tttcaaatgt gaatgctact
    13621 ttttgaccat cagggctaaa agtcggattg ctgactagct tgttatcgaa aattactgaa
    13681 cccgtgcctg tttttttgtc gaaaacaaat gtttttcctt tcgaactatg ccggtaaatg
    13741 ctttctgatt gtgtgtaaat gaggagtttg ctttcgtcat cgttgaaaaa atattcctca
    13801 atgtagagat tattgtcgcc agatatggct gtcaattgtg ctttatcgat aaatgtaccg
    13861 gtttgctttc catcagctac gctgtatatt tcgagtctgt cacctttatt gatgatgtac
    13921 gatttgccgt ctttcatgta agcaaatccc ggtaaatttt gtgttctgaa tgtccctttt
    13981 ttgaaaatat cttccaggtc aaacgtcttt aatgtctggc tgcacaaact gcccatacta
    14041 agcacgaata gtaataacaa tgctgatttt ttcatttaca tttcttttta aaattttcat
    14101 caaatctata actaatactt taatcgatta acagtttaga gcatttttag caaaattcgc
    14161 cggccgacat ttttcaaaat catacattca gcttgtacat aaatcgttta ttattgaaca
    14221 ttgatgacca cttttaaagt cataataata ttgacttcca taattaacac atttttaacg
    14281 gtgaagtata agtcgctatc attctcagga aaattcatat aaatatcttc gtcaatacgt
    14341 gtttttacgt aaccatattt taatacttat actattaccg aacctacaca atgtatattt
    14401 acgtcaaatg caatgttatt ggatttgcaa ttaatccgga aattgtccaa ctttgcaccc
    14461 gatgaagatt tttgcttttt tgttctcttt ctatatcctt gccctgtcag tagtaccttg
    14521 tacagaccag gctggttgtg gttttcagac tgagcaaaca gataatcatt ccaaagaaga
    14581 aggacaccat catgattccg aatcaaagga tcattgcaca cctttctgcg tgtgtacttg
    14641 ttgtagccag gcattcaatc ataattttta caaatcggct atcacagaac gaaaggaagg
    14701 tgtaacacac gaatgtcctg cctatacaag ctcattcatt tcagaaacat tcttccagat
    14761 ctggcaaccg cctaaaatca gctaatgaat tattaaggtt ctgcgaaacc agtacgccga
    14821 ttgatttgtg gtgaatgctc accacgaaca caatatattg ttcattataa ctgatattga
    14881 aatgttagaa aaaataattg cgttcagcat aaaaaataag tttcttgtca ttctcatgac
    14941 acttgcactt atcatgtggg gaatgataag tgctaccaaa ctcccgatag atgcggtacc
    15001 cgatataaca aataatcagg tacagatcat aaccctatgc ccaacgcttg ccagccagga
    15061 agtagaacaa ctggtgactt accccatcga acagacagtc tctaatctgc cggatgttga
    15121 agaactaaga agtatctcac gctttggcct ttcggtaata accgttgttt ttgatgaaag
    15181 tgtagatatt tatttcgcaa gacagctcat ttctgaaaaa ctaaaagaag cagcagagcg
    15241 gatacctaaa ggtattgggc agcctgaaat ggctccggta agtacaggac ttggcgaaat
    15301 ataccagtac atcattcacc caaaaaaagg aagtgaacat aaatactcgc ctgctgacct
    15361 gcgaaccatg caggactgga tagttgctcg ccaactgtat ggcacaccgg gtatagctga
    15421 ggttaacagc tttggaggcc agctcaaaca gtatgaagta gctgtcaacc ccgatagact
    15481 gctggctatg gatgtcacta ttcctgagat attcacagcg cttgaaaaaa ataatgaaaa
    15541 cactggcggt gcctatattg ataaaaaacc caatgcatat ttcatccgtg gtgttgggct
    15601 cattggttca tttgatgata taaaaaatat agtagtaaaa tcaaacccta acggcatacc
    15661 gatactaata aaagacgtgg ctgatgtacg ctttggcgga gccgtacggt atggtgcact
    15721 tacatacaat ggcgaaaaag atgcagtcgg cggtttgata atgatgctta aaggagctaa
    15781 cagtgctgaa gtggtcaacc gaataaagga aaaactacct actatacaaa aatcactacc
    15841 tgctgacgtg actattgagc catttcttga ccggacagac ctcgtgaacc gagcaatctc
    15901 tactgtagaa aagaatctga tagaaggagc actcattgta atctttgtat tggttctgtt
    15961 tcttggaaat ttccgagccg ggctaattgt agcgtctgcc attccattat ctatgctgtt
    16021 tgctcttggc atgatgaatg ttttcggtat atcagccaac ttgatgagtc tgggagctat
    16081 agattttggt cttattgtgg acggggcagt catcattgtt gaagctacca tgcaccacct
    16141 tgggctgcgg aagattacag gcagactgac acaagacgag atggataagg aagtattcga
    16201 gtcggcttcc aagataagga atagtgcagc tttcggtgaa attatcatac tgattgttta
    16261 tatacctata cttacgttga taggtacgga aggaaaaatg tttcgaccaa tggcgcagac
    16321 tgttggtttg gccattgccg gtgcactgat cctttcactc acttacatcc cgatgatgtg
    16381 ttcattgttt ctttcgaaga atatttctca caaagaaaca ttcagtgacc ggatgatgcg
    16441 gttttttcaa ggaatttata ctcctttgct cgaaaaggct atccgattca aaaatatttt
    16501 ggtagcagct acagtgctga tatttgctat tacagtattt attttttcaa gaatgggagg
    16561 cgaattcata ccaacactgc aggaaggaga ttttgcattc cactgtatat tgccacaggg
    16621 tacatctttg tcacaaagtc tggaaacatc catgcaggca tccaggatac ttcgcgagtt
    16681 tgacgaagtt agaatggtag taggcaaaac cggttctgct gaggtaccga ccgacccgat
    16741 gcctcctgaa gccaccgaca tgatggtgat actaaagcca cctaaggaat ggaaacgcaa
    16801 cataagctac aatgccctgg ctgaggaaat gatgcacaag ctcgaaagtc tgcccggtgt
    16861 tttttttgaa agcagccaac ctatacagat gcgcttcaac gaactaatga ccggtatcaa
    16921 acaggatgta gcagtgaagg tattcggcga aaatctggat actcttttgt tgtatgccaa
    16981 taagatagca cccatcatca gttcagtaaa aggaacaact gagccgagtg ttgagcgaat
    17041 tgcaggcttg ccgcagatag ttatcaggta taaccgctcg cagatggcca attatggcat
    17101 caacatccag gatatcaacc gtattgtcag tactgcattt gctggtggca gtgcaggtgt
    17161 tgtttatgaa aatgaaagaa aatttgacct tgtagtacga cttgacagta ccaaccgcag
    17221 caaaatagat gatgtaagcc acctttacat acccgcagcc aatggtacac agataccact
    17281 atcacaggta gcagaaatca aactcgaact cggcccggta cagataagcc gtgaagacgg
    17341 aaaaagacga atagtcgtcg ggttcaatgt caaagaccgt gatgtggaaa gtgtagtgac
    17401 cgagattcgc gaacagcttg ataaaaatct taaattatca gaaggttatt actatacctt
    17461 tggaggtaca ttcgagagcc ttcaggcagc atcaaagcga ctgatgattg cagtacccgt
    17521 tgcacttgct cttattttct tgctcttgta cctgacattc ggttcagcaa gccaggcatt
    17581 gctcattttt actgccatcc ctatgagtgc catcggcggt gtattcgcat tattggcacg
    17641 tggcatgccg tttagtattt ctgcaggtat agggtttata gctttgtttg gtgtggctgt
    17701 cctcaatgga atagttttga tagcaacatt taaccagtta aaaaaagacg gtatggaaaa
    17761 tattactgaa agaataatcg aaggcacaaa gataagattg cgaccggtgc taatgacggc
    17821 aacggtagct tcactcggat ttctgcctat ggcattatcg catggtgcag gggcagaagt
    17881 acaaaaacct cttgccacgg tagtgatagg cgggctatta accgctacat tccttacact
    17941 gtttgtattg ccattattat acatgatttt ctttacaggc aagagaataa gcatcaaacc
    18001 acttgcaata ttgcctttct tgatactgtt ggctgataat gttgatgcac agaaagtgtt
    18061 gacagaggaa gaagtggtca gcattgcctt aaaaaataat tccggaatac agtctcagga
    18121 tttgggcata atggcttcaa gcgaaatgcg aaaaacggtt tttgaactac ccgctactgc
    18181 attcaatgtt cagtatgggc aattcaacag tataaacaac gacataggat ttcaggtaga
    18241 tcagagcatt cagatgccgg catatttttc agcaaaaaaa cagttgtaca atgcccaggt
    18301 gcaagctgac cagatgaaga aggacatttc ggttaatgag ctgcgttcga aggtacggtc
    18361 gttgtatgct gagctccgtt atctttcatt tgccagagaa cttttgttgt cagttgacag
    18421 cctttataat gatttcatca aagtggccga tataagatac cgaaccggcg agaccaatct
    18481 gcttgagaaa atgacagccg aaaccaggcg tggacaattt tcactcatgc tccagcaaaa
    18541 tgctgctgaa tatattaaca catatcagtc actcaaagcc ctgatgtata caccggaaga
    18601 ttttgcaccg atagcgccaa aagattacac acctgcatcg ctaactatag ctgctgacac
    18661 ttcaatgctt gaaagcaacc cggcactcag atacctatac cagcagacag tagtcgccga
    18721 aaaggacaag aaatttgtaa agacagcaat gttgccagag ttatcggttg gttacttcaa
    18781 ccaatccatc acaggcgcac aaaatatcga cggaaacgaa gtgtttttcg gacgcgataa
    18841 gcgttttcag ggcataaatg tcggggtttc agtgccgctt attttttcaa gcaatgctgc
    18901 aaaactaaga tcactcacct atcgcggact tgcattacaa aaagaggcag aacagagcaa
    18961 agccttattg cagtcgcagc tgctaaatga aaaacgcaca taccaaagaa atctcgagcg
    19021 actcgaattt tttacaaaaa ccgccttgcc caatgccgac acgatgatta acactgctac
    19081 atcttcattc aggcatggcg aaattggata tatcgagtac cttcaggcat tgcagtctgc
    19141 tgctgaagta aagcttgact atcttgatac catcagacaa ctcaaccaaa atattcttaa
    19201 catcaattac ttgcttaatc aataaatccg taacaatgaa cagatatata ttaatcataa
    19261 tagtgtcact tatttcgctg ctcacttcat gccatcagca tacagaagga gacggacatt
    19321 cgcatggtac agaagatgct catgcaggtc attcgcatga gaatactgaa gatcttacga
    19381 ccacctcact taccgatgaa caaataaaat caataaagtt agagtacggt aaaattgaga
    19441 aaaaacaact cactgcatct ctcaaagcca atggattgct tcgggtaccc aacaacaaca
    19501 gagctaccgt tagttcgttg ttcagtggtg tcattaaatc tattttggta cagacaggta
    19561 agaatgtaac gaaaggtcag gttattgcca cacttaccaa tacttcactc atcaatatgc
    19621 aggaagaata cctcagcata acagcaaaac ttgaactcgc ccagctcgaa tacgaacgtc
    19681 agcaggctat gaaagaaaac aacgcatcgg cactcaaaac atttcagcag gccgctactg
    19741 aactcaaaac gctaaaagca agaaaggcaa gtctaaaaac ccagctaaat atggccggaa
    19801 tcaacacaaa gacactctcc agtgaaaata tccagtcagt attgagtatc aaaagcccaa
    19861 tcagtggagc cattagcaca cttaacatca acatcggtag ttttgtagaa acgaacacag
    19921 caattgctga aatagttgat aacagccagc tacatgtaga tctgtacgtt tacgaaaaag
    19981 acctgtacaa actacgaaat ggtcaaacta tccattttac actcaccaat aatccgggta
    20041 aagaatacga tgcagatatt tattcaatta gcaatacttt tgagcctaat accaaggccg
    20101 tttctgtaca tgctactgtt aaaggcaaca agcagggtct catagacgga atgagcataa
    20161 ctgcattggt aagcctcgaa gatgcaaccg tagaagctgt accggtgtct gccatagtca
    20221 accacgaagg ccaagactat atattcattg tgacaaatgg acctgctgca aatcaagacg
    20281 aacacgatca cgaaggccat gaccatgaag gacacaatca tgatgaccat aaccatgaag
    20341 gtcataatca tgatgaagac cataaggaga caaagaccac cgcatcacca acaggaacat
    20401 tctttgaaag aattccaata aaacgtggta ctacggacgt aggttatagc gaaatcacac
    20461 tattgaaaga tgtaccaaaa gatgtaaaaa ttgtaaccaa cggtgcattc tttattatgg
    20521 ccaaaatgac caatcagggt gaagcccacg agcattgatg gtaaaatact actatatata
    20581 ccaacatgac aaaaaataat tcaatgacat atcagtattt tgtaacttgg catatcgttt
    20641 attcattatt aatactggta tgcgttaaat aagttaagat gaaactacca ttttatgaca
    20701 gaaaattttt gctgcttatc gcagcaattg ttatcgttat tacattagag attttatcca
    20761 tagcgggcat tcacattcct atgccttatg caccttttat ttttgcttca ttcattattg
    20821 gtattgggta cgaagtactt tgggaaggaa taagagctat aggcaagctt aatttcagta
    20881 gtatcaatct gctcatgacc atcgcagtta tggcagcatt ttatctgggc gaatatcctg
    20941 aagcagctgt tgttatagta ttatacgtac tgggcgaacg ccttgaagac attggtattg
    21001 aaaactcaaa gtcggcaatg gacgagctca tcagcagagc acctaaaaaa gcatttgtaa
    21061 aattactcaa taaagaagta tttattgatg acatacagat aggtaccatc atccaggtaa
    21121 aacccggtga aatgatacca ctcgacggca aaatcatatc cggtgaaaca agcattgatg
    21181 aagccgccat caccggtgaa cccataccta aagataaaca tgccggagac aatatttttg
    21241 cagggacact caacaagaat ggttttatcg aaatagaaac cactcgtttg tctgctgata
    21301 ctacattttc aagaatagtc aggctcactt ttgaggcatc ggccaacaag agcgatactc
    21361 agaagttcat acagatgttt tcgagat
//
LOCUS       DAOQQY010000019        21121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_747_length_21121_cov_6.193060, whole genome shotgun sequence.
ACCESSION   DAOQQY010000019 DAOQQY010000000
VERSION     DAOQQY010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 21121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21121
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_747_length_21121_cov_6.193060"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..313)
                     /locus_tag="PLE23_02125"
     CDS             complement(143..313)
                     /locus_tag="PLE23_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82091.1"
                     /translation="MVKPPPETITALPPIITNRQCAQPQIPFLSSSSGHAPIGSGYPP
                     YFVPASIPHAFR"
     gene            complement(286..2133)
                     /locus_tag="PLE23_02130"
     CDS             complement(286..2133)
                     /locus_tag="PLE23_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMX82092.1"
                     /translation="MKNKLQFLIFFSFILSFSQLFAKKKYALIIGINQYYDRPGLKYS
                     TRLQGCVNDANTMKALLHNRFNFDQNDITVLTDEHATSKNVVTAFNQILQKVKAGDVF
                     VFYFSGHGVWINNMNQNKYEARLKKNMNQAIVLADLYADKLGCLYKDSDIKRQFNKFI
                     DKKVITTGIFDCCFSGHLSMMETLSMHNPYQNLNPTYAQRSISFRDVLQSHEQYNDSL
                     MTHAEQDRDSVLLNMLETGTKAFNLSDPLNIPDSVRIPRPAERPNSMFLSISGTDEYQ
                     KGEEMKDANGIHHGVFTKALLQAIDELPVNVAFSVLFNRITQIVLMNGFKQTPLHFED
                     AARLQLNLVGFNSPDNRNNPIAHIISNQKQYCTMDAGTNSDIAPGNTFTSAKNKQIKL
                     KVVSVTDENAQATITSGKSSQVNKGDQFIRTGNFVASEPLIKLYVELSPIPQATFKKI
                     LNEKMIPLSNLATYRDYKNWFITEKLQYLSLSRPDLDAVVTNHLNGFDNDPFMVFLPL
                     PQYIFSDFKSKLQQNQNYKLVQKSSEADLILYLNYTKEEGGNFVMTWYTDVMKLNGFA
                     PQFYTYNISSKHLPDNPKEISKLTTQLSDMTSELVQKYTGIWLNHHPRQ"
     gene            2301..3590
                     /locus_tag="PLE23_02135"
     CDS             2301..3590
                     /locus_tag="PLE23_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82093.1"
                     /translation="MSLLSFEYQNYKPDSALLIAQQLYDYATQKGSLFGQSLGLDGLA
                     GAFLRIGDNAKALEYYLQRLSIEEKRNIPYNVAIIYMNIANVYAQDNEQQNALEYILK
                     TDSIINHHDFKPLKLYAYLNTGNILEKADKLLEAIAYTQKSYEEAFAVKDSLILGSAL
                     NNLGNIYSKTKDYHTAIKYYRKSHPYVVAVKDNQTFSEGLLGLAKAYLAVQKKDSALF
                     YAREAYQVSYQNGILSNAMTASGYLSDFFKELKMFDSAYYYQSQLVALKDSLQSNEKI
                     KQIESLTIQEELREQHNAALKKQAEIENKQRLQLLAIGIAIPFFFLFSNFISRRKIHR
                     RAIQFSGGLSLLLLFEYITLLIHPIVVEITHHNPIFEIIIFVVLAALLVPAHHKVEHW
                     FTKKLAINYEEARVRKKIAEEIKEQQDDNSPETGLGI"
     gene            complement(3645..3881)
                     /locus_tag="PLE23_02140"
     CDS             complement(3645..3881)
                     /locus_tag="PLE23_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82094.1"
                     /translation="MKKQKFQIFHYLLAFTLSIVLYSCGQGGSTEATPPASAAPSTTT
                     EPAKVEAQTTASADSMAIDTTPMKSSQNPPPVRK"
     gene            complement(4146..4970)
                     /locus_tag="PLE23_02145"
     CDS             complement(4146..4970)
                     /locus_tag="PLE23_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82095.1"
                     /translation="MKSLIFKCSIILLVVLGFNACTKDTDDDSDYEVNSTFMEHEMSQ
                     TSNFADEAYQTGNIESGFTGGQVEVRGGGCATVTKDTLSTPKTITIDFGPTNCLCKDG
                     RYRRGKILVSYEGKYKEAGSVHTITFDGYYVNDNHIEGSKTVTNIGPDAQGRIQYAIT
                     ESIKITMANGKYFERSSSRTRTWVKGFDTPSKADDEYEITGTNTTSNSKGYGFNAEIT
                     TPLLVSADCDNIKAGIVVVVPKNPVKPQISIDFGDGTCDDLATVTINGKTHIIHLK"
     gene            5166..6392
                     /locus_tag="PLE23_02150"
     CDS             5166..6392
                     /locus_tag="PLE23_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX82096.1"
                     /translation="MNKQNTAVPVKLIIVASLGYFVDIYDLIVFNVVKKESLEALGYY
                     GDAFKTQEISLFNYQMFGMLLGGILWGVFGDRKGRISVLFGSIFLYSAANIFNAFVTD
                     VGSYAVCRFIAGIGLAGELGAGITLISETMHREKRGYGTMIIVTFGALGAVAASLVGN
                     TFGWKMTYIIGGVMGLALLLARAGVLESGMFKRLDHEKRSKGDFFMLFRGRKIFLKYM
                     ASIMIGLPVWFVVAILFAFSNQFAEINTVQGGTVLVSKSVMYGYIGLSVGDLLSGLLS
                     QWFRSRKKIVLLYLALTAVVSLIFLFAKNLDITVFYFLIFVLGVATGYWALFVTMASE
                     QFGTNIRSTVTTTAPNFVRGMVVPMTLGYKYLVPGSGIIQAALVVGIVTILIAVVSTL
                     YVEESFSKDLDYLEEA"
     gene            6527..8038
                     /gene="atpD"
                     /locus_tag="PLE23_02155"
     CDS             6527..8038
                     /gene="atpD"
                     /locus_tag="PLE23_02155"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMX82097.1"
                     /translation="MANIGRIKQIIGPVVDVAFDTPGSTIPKILNALVVKRPEGDLVL
                     ECQRHLGEDSVRAIAMDATDGLTRGMEVVDTGETIAMPVGIEIRGRLFNVIGEAIDGI
                     GQVSKAQSYPIHRNPPRFEDLSTETEILYTGIKVIDLVEPYQKGGKIGLFGGAGVGKT
                     VLIQELINNIAKGYDGMSVFAGVGERTREGNDLLREMIESGIVKYGKEFIHSMEQGGW
                     DLSKVDKELLKDSQATFVFGQMNEPPGARARVALSGLTVAEYFRDGDMSDPTGGKDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLGTEMGLMQERITSTKRGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLDRKIAALGIYPAVNPLDSTSRILDPKIIGDEHY
                     NCAQRVKGILQRYNELQDIIAILGMEELSEEDKLVVHRARRVQRFLSQPFHVAEQFTG
                     LKGVLVPIEETIRGFNMIMDGEVDEYPEAAFNLVGTIDQAIEKGKKLLAEATA"
     gene            8057..8308
                     /gene="atpC"
                     /locus_tag="PLE23_02160"
     CDS             8057..8308
                     /gene="atpC"
                     /locus_tag="PLE23_02160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HMX82098.1"
                     /translation="MLKLIVLTPESEYFQGNVKSVKVPGVSGQFEILANHAALLSALS
                     KGKVRIIKEDGQKETFEIESGFIEVLRNEVALLVRGISN"
     gene            complement(9020..10576)
                     /locus_tag="PLE23_02165"
     CDS             complement(9020..10576)
                     /locus_tag="PLE23_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HMX82099.1"
                     /translation="MKNISTFRLWLLAGTFISITTLDVSAQHAPKDNQTVTITVESKD
                     KNGVVTTKKIVRTDENLTETEIQKITDELVKEYDGKDAKIQVNVEEQKAVTADSPDKD
                     QEVIIKKGYGNDMPDDVHIFIDGGMLTDTGRLGENFFSRIFPDSLSKSIGINLDSLVK
                     NLDTKFGKFRTLTRQPFLGVTTDESIQNEQGVPVTEIFEGSAAEKAGLKINDIITNID
                     GIKVSNFAEMIREIKKHKVGDQITIGYLREGTASTATATLGEKEGNYMPFPGGEFDFN
                     HFKWNPENTPGEDNFSQRRLRRFNADGFYNEAKSDKAQLGVMVENNNDNGVKITDIVE
                     GTAAFHTGLKKGDIIEKINKERINSPEILISVVSAMKPGDEIALTYRRDGKKKTISLV
                     LGAKKESGYYGFEPEMKNNRKTYIFKNKNTTSDVQTDAPLLILENFELFPNPSKGNVN
                     ISFKSESDEDATVKILSLDGKEIQRESLKGRNGKFEKTINIKDTKPGAYIVRIEQDGK
                     MITEKFILNR"
     gene            10777..12306
                     /locus_tag="PLE23_02170"
     CDS             10777..12306
                     /locus_tag="PLE23_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMX82100.1"
                     /translation="MNKKAVFITIGLMTLALIGLIVLQLNWINWSLKLSEQQFDKNVF
                     SALNKVADRLQAKENDEALNYINGYEATYLEGQATKLLDDSVITDEDAKNSTEYQKNE
                     FTLPDNNFSRMLIAENNCSCTKCQFERWFKYNRIVNNYRIRSSVPLAERIELATLDRV
                     LKEELTNTGIDIQYNYGVYDSEHHNFIITDNHYVVAPMGQTKASFQAGGDDHLKKSKY
                     QVSLFGLSDKSPGSLMVYFQGRANALWSGIWKTVLASMFFTLIVLGGFIYTVIVIFQQ
                     KQVSEMKNDFINNMTHEFKTPLATISLAVDSITNPQIISNPDKVKRFVNIIKQENTRM
                     NGQVEKVLQMAIIDKKDFNLNFSKIDIHDIINKAVDNFALQISRREGVINTNLTASNH
                     EIFADQTHISNIIYNLLDNANKYSGEKPEILITTKDARDGVEIIVKDNGIGISKEAKK
                     HIFDRFYRVHTGNVHDVKGFGLGLSYVKTMTQAHNGHIDVISEPGKGSSFIITLPVRQ
                     S"
     gene            12360..13076
                     /locus_tag="PLE23_02175"
     CDS             12360..13076
                     /locus_tag="PLE23_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX82101.1"
                     /translation="MERSLKILLVEDDQNFGDVLKSYLEMHDYEVSLATDGVAGWEKF
                     RKNTYDLCILDVMMPKKDGFTLGKEIREKDSEIPIIYLTAKTMKDDILQGFKMGADDY
                     ITKPFNSEELLYRIQAILKRKLPKPDPLDDVKEFTIGEYHFNYPLRILSFNGTVPEGE
                     ESSFKLSPKEAHLLALFCKRINNVLPRSEALSRIWGEDNYFTARSMDVFVTKLRKYLK
                     LDPKIEIVNIHGNGFQLFIR"
     gene            complement(13094..15103)
                     /locus_tag="PLE23_02180"
     CDS             complement(13094..15103)
                     /locus_tag="PLE23_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82102.1"
                     /translation="MKQFFFTLILLTGIAQLSLGQKMSWRDYSDIAEKSFKAGDYGTA
                     AKNYKAAWLKKKSKTDLLYKAGQAYYLVKNYKEAANAFEKVSKKSSKYPVSTIEYANA
                     LKQSGAYEKSIKAYESYMKAYNGDDRPQQQELVNREIEGSKQALRMLSDSTTSAYKIS
                     YPGMVINTGFTEYAPVPYGENVLYFSSNRKGSSKIYKSEWDGSSWSKPTVPPIFPKDE
                     KEHFGNGSFSYDFKRFYYTRCKEVETKDGLRAKCDIYVIMRKGDKWGEPIKLRDYIND
                     SVATTTQPSVCKFANKEVLYFSSDRSGGYGGQDIWYCERDLSTDDLDFTVPRNCGPKV
                     NTFMDEVTPWYDIYEGKLYYSSNGQIGAGGLDVCSNMGFGKEWKETVNLGFPVNSPAD
                     DFYFIKSGDLNSGYLVSNRIFGDLKKTTDNEDIFRFEYTNFQNLFTGKIFKAQTDSLL
                     TDVRIAVYQLFPDGSESLVDNRHSPDGTFSFALDASKSYRAKVEKFEYAPAFFRFTTS
                     DVKANKLTQNFSLEKEKEASIAGTEPTLIQQPKTENIKKTEKKTPVKEKPKQEPKPAK
                     PEPKAEEPYVHRPPSFIKEGEYYLIQLAAARYPDIMSGAFEKAKQYGTIEHEPVPNQE
                     MERVLLAWWADRKDAIKTLASVKRLGFKDAFVTLHKDGERKNIVK"
     gene            complement(15151..15714)
                     /locus_tag="PLE23_02185"
     CDS             complement(15151..15714)
                     /locus_tag="PLE23_02185"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMX82103.1"
                     /translation="MKRIAIFASGTGTNAANIIRYFDDNHDISVKAVLSNNPTAGVLN
                     VAKEAGVDSLVFTREEFYETDKVIRYLLDNQIDLVVLAGFLWLVPELFIQSFPKKIIN
                     IHPALLPAFGGKGMYGARVHQAVKAANKQETGITIHYVNPHYDEGDIIFQTSCSLSDD
                     DTPDTIAAKVHKLEYEFYPKVIGQLLN"
     gene            15926..18043
                     /locus_tag="PLE23_02190"
     CDS             15926..18043
                     /locus_tag="PLE23_02190"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HMX82104.1"
                     /translation="MKFKNPVLFSFIIGAFALFAFLPKADVDPTQKETVLLQTLIQGL
                     EQLHYNPRTLDDSFSQDLFNLYIERLDGAKRFLTQAEVDKLKPFEKQLDDQIKRTDVS
                     FFNTSTDLLTNGINRAEKIYKEILEKPFDFKQNESIETDAEKRKFPANEKELKEVWRK
                     MLKYETMTRLADKLDEKAKGTAEYKDKSEAQLEDDARKIVRERFETYFDQLKKLKREE
                     QFADFLNTIANLYDPHSEYYEPVEKQSFDIKFSGRLEGIGATLQTDKEFTKIAELVVG
                     GPAWKQKELKVGDLIMRVAQGDEEGISIAGMNLNEVVAKIRGPKGTKVRLTVKSIDGT
                     TKEISIIRDEVIIDEGYAKSVILNKEGAENVGFISLPRFYADFNKRDGRQCFQDVAKE
                     IEKLKAANVNGIIIDLRNNGGGSLNDVVKMTGLFIEKGPIVQVKTRLGDAEVQQDYDS
                     RVQYGGPLIIMVNNFSASASEIMAAALQDYSRAVIVGSNHTFGKGTVQRFFDLDNAVN
                     GYDNLKPLGDVKITLQKFYRINGGSTQLRGVSSDIVLPDNFQDLKLGEKESKYAMPWS
                     QIDKLNYSQNVYNIGNVSELAAQSKERVGSNEIFSKIAANAKRLKKQRDQSVYSLNIE
                     EYQNLKKNIEKESEQIKKLSETEIPGLAVSNLNVDLQSINSDSTKINRNAEFLKDMKK
                     DIYVDETLNIMADLIRKNLASKN"
     gene            18143..20002
                     /locus_tag="PLE23_02195"
     CDS             18143..20002
                     /locus_tag="PLE23_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMX82105.1"
                     /translation="MNYLTLEHVTKTYGEKTLFEDINLSVNKGDKIALVAKNGSGKTT
                     LLRVIAGEESGEGEKCSIQLHKDVKTRFLSQEPEFHEGDTVIEALLSGDSEQSRVIRR
                     YEILLDNPGDGTELQAVMARMDDLKAWDYESRVKEILHRFNIRDFDKPVALLSGGQKK
                     RVALARLIIEEPEFIILDEPTNHLDLDMIEWLEDYLKQPGITLFMVTHDRYFLDRVCN
                     AIVELDAGHIHRYSGNYEAFLEKKAEREENEAVVLGKTKQLFKKELEWMRRQPKARTT
                     KAKSRIDEFEVINEKAHVRKDDSRVQIDLQSNRLGSKILELHYISKTYGKQKLIEDFF
                     YKFNKKDRVGIVGPNGVGKSTFLRLITSQERIDAGKVITGETTVFGYYTQDGLNLKSD
                     KRVIDVIQDIAEYVPLEKGKKMSAAQLLERFLFSRAQQQVYVSQLSGGERRRLYLLTI
                     LMQNPNFLILDEPTNDLDILTLNVLEEYLEEFPGCLIVVTHDRYFLDKIVDHLFVFEG
                     NGKIKDYNGKYSDYRWEDKQRKKEEKAEKSPEQKPAIKPTGMLSNEERKEMMRLERDI
                     EKLEAEKLAITEKFNDTSMPLEEITQLANKLEELKTLQETKEMRWMELCEKAG"
     gene            20132..>21121
                     /locus_tag="PLE23_02200"
     CDS             20132..>21121
                     /locus_tag="PLE23_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82106.1"
                     /translation="MKTQPNYQKEFRFLRNIILFNGGVKFYFPPRWQLILIFAMLFIV
                     NPRLLFSQCETPDPSDELFEYYVSLLPSQNNYRLARASGDPASIPVSFTVVRNTEGSS
                     TDVSVSQIDLTIDTLNKRFAPVGISFYRLGNVNYIDWTPLYLNSIIDGARYSYLSTAI
                     NVMVHPLQASVATQPIMTDNPDYYTLEDNIIWVEPSALWGNFASFSAAFIHEVGHIFG
                     LLHTHGATTVYHSPPDSAQVDYPYPNEKNDNSKYGRELQIRDTTYGIAFPYPNWMVAG
                     DLCGDTYPSVMNNNLYFPGWSIDCPYNGTYVDYNNDPVTPQEKNIMSYFILNGC"
BASE COUNT         6103 a         3968 c         4620 g         6430 t
ORIGIN      
        1 cttaaaaaac caaagccccg gatcataaac gaacggggct tttgcaatcc ggtgtgcaag
       61 cgacaccggg ctggggcgtt tgctcttagg tggattagtg aatgtgtgga tgatttttag
      121 tctctgtgtt taatattcaa acttatcgga atgcatgagg gatagaagcc ggcacgaagt
      181 aaggcggata gcccgacccg ataggggcat gccctgaaga agatgaaaga aatggtatct
      241 gtggttgtgc acattgtctg ttggttatga taggtggtag cgctgttatt gtctcgggtg
      301 gtggtttaac catataccgg tgtatttttg tacgagctca ctggtcatgt cgcttagttg
      361 agtcgtcaac ttagagattt cttttgggtt gtcgggtaga tgcttagagc tgatgttgta
      421 ggtgtagaat tggggggcaa agccgttgag tttcattacg tctgtgtacc aggtcattac
      481 gaagttgcca ccttcttctt ttgtgtaatt gaggtaaagt ataaggtcgg cctcggatga
      541 tttttggact aatttgtaat tttggttttg ttggagtttg cttttaaaat ctgagaagat
      601 gtattgtggt aaaggaagaa aaaccatgaa cggatcgtta tcaaaaccat tgagatggtt
      661 ggtaacaact gcgtcaaggt ctggcctact tagggaaagg tactggagtt tttcggtaat
      721 gaaccaattt ttgtaatcgc ggtaagtagc caaattggaa agaggaatca ttttttcatt
      781 taatattttt ttgaatgtgg cctgcggaat tggtgagagt tcaacataga gttttatgag
      841 aggttcggag gctacgaaat tgccggtacg gatgaattga tcacctttgt taacctgcga
      901 acttttgccg gatgttatgg tagcctgggc gttttcgtcc gtgacactaa ctacttttaa
      961 ctttatctgt ttgtttttag cagatgtgaa tgtattgcca ggagcaatgt ccgaatttgt
     1021 acctgcatcc atagtacaat attgcttttg gttggatatg atgtgtgcta taggattgtt
     1081 tctgttgtcg ggcgaattga agcctaccag atttaattgt aaccttgctg catcttcgaa
     1141 atgcaagggt gtctgtttga atccgttcat gaggactatt tgagtgattc tgttgaacaa
     1201 gacactaaaa gctacattta ccggcagttc gtctattgct tgaagcagag cttttgtaaa
     1261 tacgccgtga tgaatgccgt tggcgtcctt catttcttca cctttctggt attcgtcggt
     1321 gcctgagatg gacaggaaca tcgaattggg cctttctgca ggtcggggaa tgcggactga
     1381 atcggggatg ttgaggggat cggaaaggtt gaatgctttg gtaccggttt ctagcatgtt
     1441 caaaagtacc gaatcgcgat cttgttcggc atgtgtcatg agcgaatcat tatattgttc
     1501 atggctttgc aatacatctc tgaatgagat ggaacgttgg gcataggtgg ggttgaggtt
     1561 ttggtagggg ttgtgcatag agagtgtttc catcatggac aggtgcccgc tgaagcaaca
     1621 gtcaaaaatg ccggtagtta ttactttttt atctataaat ttattgaact gtcgtttaat
     1681 gtccgagtct ttgtacaaac acccgagttt atcggcatat aaatcggcca gtactattgc
     1741 ctgattcata ttttttttca gtcttgcttc atatttattt tggttcatgt tgttgatcca
     1801 tacgccatgt cctgagaaat agaacacaaa gacgtcacct gctttgactt tttggagaat
     1861 ttgattgaat gcggtgacta catttttgct tgttgcatgc tcatctgtga gtactgttat
     1921 gtcattctgg tcgaagttga atctgttgtg taacaaagct ttcatggtat tggcgtcatt
     1981 gacacaacct tgcaatcttg tactgtattt taagcctggt cggtcataat attggttgat
     2041 accgatgatt agcgcatatt tttttttagc aaaaagttgg ctaaaagaaa gaatgaatga
     2101 aaaaaatatc agaaattgta atttattttt catgctctac aaattttaaa tttaaatata
     2161 aataagtgcg ctacaaaagc ctgtttttaa ttttgctttg tataactatt tgtcactcgg
     2221 ctataagtca ggataaatat agagatagtt tgattatttt aatgaatact acttcttcag
     2281 attcgttaaa aatgcaaaca atgtctttgt taagcttcga atatcagaat tataaaccag
     2341 attctgcttt gttgattgcg caacaattgt atgattatgc tacacagaag ggaagtcttt
     2401 ttggtcagtc acttggtttg gacggactgg caggtgcttt tctaaggata ggtgacaatg
     2461 ccaaggcact ggagtattat cttcaacggc tgagcattga agagaaaaga aatataccat
     2521 ataatgtagc catcatctat atgaatattg ccaatgttta tgcccaagac aacgagcagc
     2581 agaatgctct tgaatatata cttaagacag attctatcat taatcaccac gacttcaaac
     2641 cattgaaatt atatgcttat ctcaatacag gcaatatact tgaaaaggca gataagcttt
     2701 tggaagcgat agcttataca caaaagagtt atgaagaggc ttttgctgta aaagatagcc
     2761 tcattcttgg ttcggccttg aataatttag gaaatattta ttccaaaact aaggattacc
     2821 acacggcaat taaatattac aggaagagtc atccttatgt agtggctgta aaagataatc
     2881 agacattttc ggaaggtttg ctggggctgg caaaagctta tttagctgtt caaaaaaaag
     2941 attcggctct attttatgcg agagaagcat accaagtttc gtaccagaac ggcatcttgt
     3001 caaatgctat gacagccagt gggtatttgt cggatttttt caaggaacta aaaatgttcg
     3061 atagtgctta ttattatcag tcacaattgg tagcccttaa ggattctcta cagagcaatg
     3121 aaaaaattaa acaaatcgag agcctgacca tacaagaaga gcttcgcgaa cagcacaatg
     3181 cagcattgaa gaaacaggca gaaattgaaa acaagcaacg actacagcta ctcgctattg
     3241 gtatagctat tccgttcttc tttttgttta gtaattttat cagccgacgg aaaatacata
     3301 ggcgtgcaat ccagttttcg ggaggcttat ctcttttgtt gttgttcgaa tatatcacct
     3361 tgttgattca tccgatagtt gtagaaataa cccatcataa tcccatcttt gaaattataa
     3421 tattcgtagt cctggcagcc ttattggtgc ctgctcacca caaggtagaa cactggttta
     3481 ctaagaaact cgccatcaat tatgaggaag caagagtcag gaaaaaaatt gctgaagaga
     3541 ttaaggaaca gcaggacgat aattcaccgg aaaccggttt gggtatttaa tttcccgaac
     3601 acgaacatct tcctttggat aatctcttca gctttgtaac ttaattattt ccttacaggt
     3661 ggtggatttt gagaagactt cattggcgta gtgtctatag ccatagagtc ggcacttgct
     3721 gtggtttgtg cttctacttt tgcaggttcg gtagttgtgg atggtgctgc tgaagccgga
     3781 ggtgtagctt cagttgatcc accttgccca caagaataca agacaataga caatgtgaag
     3841 gccagcaagt aatgaaagat ttggaatttc tgttttttca tgattggtta cttttttagg
     3901 atagtgattg tttgagaatg attaaaatat atcattgcaa aactaaaaaa tatttttcat
     3961 tttaattgaa attataaaat attcagaaaa tatgtacaat attttttgtg atgcttaata
     4021 aatgttttgg acactgttta ggaaatctga atgcaaacca aatctatcca aaaatataaa
     4081 agtgcacccg gactcaaagc cggatgcact cgatgaggaa tgaatatatt tgtcttgctt
     4141 caattttact tgaggtgtat gatgtgggtt ttgccattga tggtgactgt ggcaaggtca
     4201 tcgcaggtgc cgtcaccgaa gtcgatgctt atctgtggtt ttaccggatt tttgggaacc
     4261 actactacta taccggcctt tatgttgtcg cagtcggctg atacgagcaa tggtgtggtt
     4321 atctcggcgt tgaatccgta gccttttgag ttgctggtgg tgttcgtgcc ggtgatttcg
     4381 tattcgtcgt cagctttgga aggtgtgtca aaacctttta cccaagtgcg tgttcggctc
     4441 gaactgcgct cgaagtattt accgtttgcc atcgttattt taatagattc tgtaatggca
     4501 tattgtattc ttccttgtgc atcggggccg atgtttgtta cagttttgct gccttcgatg
     4561 tggttgtcat tgacgtaata tccgtcgaat gtgatggtat gtaccgaacc ggcttctttg
     4621 tatttgcctt cataactgac caatattttg ccccttctat acctgccgtc tttgcaaagg
     4681 cagttggtcg gcccgaagtc aatcgtgatg gtcttgggtg ttgataaagt atctttggta
     4741 acggtagcac agccaccacc ccgtacttct acctggcctc ctgtgaagcc cgattcgatg
     4801 ttgccggttt ggtatgcttc gtctgcaaaa ttgctggtct ggctcatttc gtgttccatg
     4861 aatgtgctgt tgacttcgta gtcggagtca tcatctgtgt ctttggtgca ggcattgaag
     4921 cccaatacaa ctaatagtat gatactgcat ttaaaaatta gtgatttcat agttttaaga
     4981 ttttggtact ttgattgttt tgagagcaaa tggtttaacc gttaagaaat tttttttttg
     5041 gaaaatgtat tggtggagca tgaattatta acagagggca gtcagtaagc cgaaaaacca
     5101 ttaaattgtg gcctgattca ttaccgatat tttgtgtaaa tcaatatcag aaacaaacca
     5161 acacaatgaa caaacaaaac accgcggttc ctgtcaaact tatcattgta gcttcactgg
     5221 gctactttgt agatatatac gatctgatag tattcaatgt ggtaaagaag gaatcgctcg
     5281 aagcactggg ttactacggt gatgctttca agactcagga aatatcgctt ttcaattatc
     5341 agatgttcgg tatgttgctg ggtggcatac tgtggggcgt ttttggagac aggaaaggac
     5401 gcatctcggt gttgttcggt tctattttcc tgtattctgc agctaatata ttcaatgctt
     5461 ttgttacaga tgtcggcagt tatgctgtgt gtaggtttat agcaggtata ggtctggccg
     5521 gagagcttgg tgcaggcatt acactaatat ctgagaccat gcaccgtgag aagcgtggct
     5581 acggtacaat gatcatcgtt acgttcgggg cactcggcgc ggtagcggct tcattagtag
     5641 gtaatacctt tggctggaag atgacctata tcattggtgg tgttatgggt cttgcattgt
     5701 tgctggcgcg tgccggtgta ctagagtcgg gcatgttcaa gcggcttgat catgaaaagc
     5761 ggtcaaaagg tgattttttt atgttgttca gaggcaggaa gatatttttg aaatatatgg
     5821 caagcatcat gattggtttg ccggtatggt ttgtggtggc catcctgttt gctttctcca
     5881 atcagtttgc ggaaatcaat actgtccaag gtggtacagt acttgtatcc aagagtgtga
     5941 tgtatggtta tatcggtctt tcggtgggcg atctgctgag tggattgctt agccagtggt
     6001 tcaggagcag gaagaagata gtgcttctgt accttgcact cacggccgtg gtgagtctga
     6061 ttttcctgtt tgccaagaat ctggacataa ccgtctttta tttcctcatt tttgtattgg
     6121 gtgtagctac gggttactgg gctttgttcg tgacgatggc ttctgagcag tttggtacca
     6181 atatccgttc gacggtgact actacggcac ctaattttgt aagaggtatg gtggtgccta
     6241 tgacgcttgg gtacaaatat ttggtgcccg gtagcggtat catccaggca gcccttgtag
     6301 taggaattgt taccatcctc attgctgtgg taagtacact gtatgtcgaa gaaagctttt
     6361 ctaaagacct tgattacctt gaagaagctt aagtgccaaa tttttataaa atatttcaaa
     6421 tttttaagct ttaattccaa atattctttg gtgagaaatc taaaatcatt atctttgcga
     6481 cgaatttcag aaagctattc aatttcacta aaaatcaaca tgttttatgg ctaatattgg
     6541 tcgcattaaa cagataattg gtccggttgt ggacgttgcg tttgacactc cagggtcaac
     6601 tattcctaag atattaaatg cacttgtggt aaagcgtcct gaaggagacc ttgtacttga
     6661 gtgccaaaga cacctgggcg aagatagtgt cagggcaatc gccatggatg ctactgacgg
     6721 gcttacccgt ggtatggaag tggtagatac aggagagaca atcgctatgc ctgtaggtat
     6781 cgaaatcaga ggccgacttt ttaacgtcat cggtgaagcc atcgatggta taggtcaggt
     6841 ttcaaaagca caaagctacc cgattcacag aaatccgccg agattcgaag atctctccac
     6901 agaaaccgaa atactttaca caggtattaa ggttattgac cttgttgagc cttatcagaa
     6961 aggtggtaaa attggattat tcggtggtgc cggtgtagga aagactgtgt tgattcagga
     7021 attgatcaat aacatcgcaa agggttacga cggtatgtcg gtgtttgcag gtgtgggtga
     7081 acgtaccaga gaaggtaatg acctccttcg tgaaatgatt gaatcaggaa tcgttaaata
     7141 cggtaaagag ttcattcatt cgatggagca aggtggctgg gacctttcaa aggtagataa
     7201 agaattatta aaagacagtc aggctacttt cgtattcgga cagatgaacg aacctccggg
     7261 agctagagct cgtgtggcac tttctggtct tacagtagct gaatatttcc gcgatggtga
     7321 catgagtgac ccaacaggcg gtaaggatat ccttttcttt attgacaata tattccgttt
     7381 tactcaggca ggttctgagg tatcagctct gcttggtcgt atgccttcag cagtaggtta
     7441 tcagccaacg cttggtactg agatgggtct gatgcaggag cgtatcactt ctacaaagcg
     7501 tggctctatc acatctgtac aggctgtata tgtacctgcg gatgacttga cagacccggc
     7561 tccggctaca acatttgccc accttgatgc tactacggta cttgaccgta agattgcagc
     7621 tttgggtatc tatccggcag taaacccgct tgattctact tccagaatcc tcgaccctaa
     7681 aatcatcggc gacgaacact acaactgtgc tcaacgtgtg aagggtatcc tgcagagata
     7741 caacgaacta caagatatca tcgccatctt gggtatggaa gagctttcag aagaagacaa
     7801 actcgtagta caccgtgcaa ggcgtgtgca gagattcctt tctcaaccat tccacgtagc
     7861 agaacagttc accggtctta aaggtgtact tgtaccgatt gaagagacca tcagaggttt
     7921 caacatgatc atggatggtg aagttgacga atatcctgaa gcggcattta accttgtggg
     7981 taccatcgac caggctattg aaaaaggtaa aaaattattg gcagaagcca cagcttaatt
     8041 gtaatccaga taagatatgc ttaaactaat cgttttaacc ccggagtcag aatactttca
     8101 aggcaacgtt aaatcggtca aggtacctgg tgtgagtggt cagttcgaaa ttcttgccaa
     8161 tcacgctgca ctcctatctg cacttagcaa gggaaaggtc agaattatca aagaagatgg
     8221 ccagaaggag acattcgaaa tagaaagtgg cttcattgaa gtactaagaa acgaagtagc
     8281 attactcgta agaggtatta gcaactagtt atattcccaa aaataattca aacccggcaa
     8341 gactcaagtt ttgtcgggtt tgcttttttt gcataaagcc ttacctgggt ttaattttgg
     8401 tagaaatgat tgtaccacaa ttgtacatcg ctttatacgc aacggtttac tttggtatct
     8461 attattggtc aggacaaagc taaagcaacc ggtattctct gaatgagagt gcaataatga
     8521 tgctcaggct ggctattata agccaaatgc gagggtctcg gatgaagtct gattgttgtt
     8581 gtcttaattc cacgggtctg ttttttttaa tgttaacaat gaccggtgat tggggtatct
     8641 gttggtacag ttaatttttt ctctttctgt attgttaggc atatactgtg ccatgaagtg
     8701 ctgcatcagt cagattaagt gacaggaaaa atggtgcagc tcttactaca atacagttgt
     8761 catccggtca atgtgctata agctttatat gtattcatgt tattgacagt gttatataaa
     8821 gtgtacaata caataatctg tgcttgtatt tttattgatg gttatatgtt gatgtttttc
     8881 aaggctgtaa tagagcttgt ttgttacaat ttctacatac tgatttgcct atattttttc
     8941 gtacaaataa atacaaaaaa gaataaaaaa acccggaaac aaaggacgtt cccgggccaa
     9001 ataatgaaat tatgaaaact tatctgttga gaataaactt ctcagttatc attttaccgt
     9061 cctgctctat ccttacgata taagctcccg gtttagtatc ttttatattg atggtttttt
     9121 cgaatttacc gtttctgcct ttaaggcttt cacgctgtat ttccttacca tcaagactca
     9181 atatttttac tgttgcatct tcgtctgact ctgacttgaa gctgatgttt acattaccct
     9241 tgcttgggtt aggaaaaagc tcaaagtttt cgagtatgag tagtggtgca tctgtttgaa
     9301 cgtcagatgt ggtgtttttg tttttaaaaa tgtaagtttt gcgattattt ttcatttctg
     9361 gctcgaaacc atagtagccg gattcttttt ttgcacctaa cactaatgag atggttttct
     9421 ttttgccatc gcgtctgtag gtaagtgcta tctcatcacc gggtttcata gcagaaacga
     9481 ccgaaatcaa tatttcagga ctattgatac gctctttgtt gattttttcg atgatgtcgc
     9541 ctttcttcag tccagtgtga aaagcggccg tgccttctac aatatctgtg attttgacac
     9601 cattatcatt gttgttttca accatgacac cgagctgtgc tttgtctgat tttgcttcgt
     9661 tataaaaccc atctgcatta aatctcctga gccttctctg gctgaagttg tcctcgcctg
     9721 gtgtattttc gggattccat ttgaagtggt taaaatcaaa ttcaccaccc gggaaaggca
     9781 tgtagttgcc ttctttttca cctaaggtag ctgtggcagt agatgctgtt ccttctctca
     9841 agtagccaat ggttatttga tcacctacct tgtgtttttt gatctcgcgg atcatttctg
     9901 cgaaattgct taccttaatt ccatcaatat tggtaatgat gtcatttatt ttaagaccag
     9961 ctttttcagc tgccgagcct tcgaaaattt cagttacggg cacaccttgt tcattttgga
    10021 tgctttcatc tgttgttaca cccaaaaatg gttgccgggt aagtgtcctg aatttgccaa
    10081 attttgtatc tagattttta accagagaat ccaagttgat accaatgctt ttactcagac
    10141 tgtcagggaa aattctgctg aaaaaatttt ctcccaatct gcctgtatcg gtcaacattc
    10201 caccgtcgat aaagatatgt acatcatccg gcatatcatt tccgtaaccc tttttgataa
    10261 tcacctcctg gtctttgtct ggagaatcag cagttacagc tttttgttct tcgacattta
    10321 cctgtatttt ggcatctttg ccgtcgtatt ctttaacaag ttcgtctgta attttttgta
    10381 tttctgtttc agtaagattt tcatcggttc gcactatttt ctttgtagtg actacaccgt
    10441 ttttgtcctt tgattctaca gtaattgtaa ctgtctggtt gtctttaggt gcatgctgtg
    10501 cactgacatc cagtgtggtg atacttataa aagtacctgc caataaccat aatcggaaag
    10561 ttgaaatatt tttcacaata caaatttgat gataattttt gaataaatca atatcataca
    10621 taaaaacagc aaaattaatg agtaatatat aatgtttgcg ttaaaaaata gttaaataac
    10681 ggactcacta cagtcagaga cgttgtaaga tgttgttttg agtatatttt ataaatttgt
    10741 atctttacaa actgtaatgt accgtataat atcaggatga ataaaaaggc tgtatttatt
    10801 accattggct tgatgacgtt ggcccttatc gggctaatcg ttctgcaact caattggatt
    10861 aactggtctt tgaagcttag tgagcagcag tttgacaaga atgtcttttc ggccctcaat
    10921 aaggttgctg acagattgca ggcaaaagag aatgacgaag cactcaatta tatcaatggt
    10981 tatgaggcaa cttatttaga gggtcaggct acgaagttac tcgatgattc ggtaataaca
    11041 gatgaagatg caaaaaattc taccgaatac caaaagaatg aatttacgct gccggataat
    11101 aatttttcac gtatgcttat tgccgaaaat aattgttcgt gtactaaatg ccagttcgaa
    11161 cgctggttca agtataaccg gattgtcaac aactatcgca ttcgcagttc ggtaccgctt
    11221 gctgaaagaa tagaactcgc aactttagac cgcgtactga aagaagagct caccaacacc
    11281 ggcatcgaca tacagtataa ttatggtgtc tatgatagcg aacaccataa ttttatcata
    11341 acggacaatc actatgtggt agcaccgatg gggcagacta aagcttcgtt tcaggctggc
    11401 ggtgatgacc accttaaaaa atcgaaatat caagtgagct tgtttggcct gagcgacaaa
    11461 tcgcccggtt cgcttatggt ttattttcag gggcgtgcca atgccctgtg gagtggtatc
    11521 tggaaaaccg tccttgcctc aatgtttttc acattgattg ttcttggggg ttttatctat
    11581 acggttattg ttatttttca acagaagcaa gtgtctgaaa tgaaaaatga ctttatcaac
    11641 aacatgaccc acgaattcaa gactccgctt gctactattt cacttgctgt tgattcaatc
    11701 accaatccgc agatcatctc caatcctgat aaagtaaaac gcttcgtcaa tatcatcaaa
    11761 caggaaaata cccgaatgaa cgggcaagta gagaaggttc tccagatggc cattattgat
    11821 aaaaaagatt ttaacctcaa tttttctaaa attgatatac atgatatcat caataaggct
    11881 gttgataatt ttgcattgca gataagcagg cgcgaaggtg taatcaatac caatcttact
    11941 gcttccaatc atgaaatttt tgccgaccag acgcacatct ctaatataat ctacaatctc
    12001 cttgacaacg ctaataagta ttcgggcgaa aaaccggaaa tactgataac aaccaaagat
    12061 gcgcgtgatg gagtagaaat aatagtcaaa gacaacggta tcggtatttc aaaggaagca
    12121 aaaaagcata tttttgacag attttatcgt gtacataccg gcaatgtcca tgatgttaag
    12181 ggttttggcc ttggtctaag ttatgtaaaa accatgacac aggcacataa tggccatata
    12241 gatgtcataa gcgaaccggg aaaagggtca agttttatca taacattgcc cgtaagacaa
    12301 tcataaatac ttttcttgcg ttataaatgt taacgattat cctaaaaggt taaaatatta
    12361 tggaaagaag tttaaaaatt ttactcgtag aagatgacca gaatttcgga gatgttctga
    12421 agtcttatct agaaatgcac gattatgagg tatcactggc tacagatggt gtcgccggtt
    12481 gggaaaaatt tcgcaaaaac acctacgacc tgtgtattct ggatgtgatg atgcccaaga
    12541 aggatggttt tacactcggc aaggagattc gcgagaaaga ttccgaaatt ccgattatct
    12601 atcttactgc caaaaccatg aaggacgaca tactccaggg tttcaaaatg ggagctgatg
    12661 actatattac caaacctttc aattccgaag agttacttta ccgcatacaa gctatactga
    12721 aacgaaaatt gccaaaacca gatcctcttg atgatgtaaa ggaatttacc ataggtgaat
    12781 accattttaa ctatcctttg cgcattcttt cctttaacgg taccgtacct gaaggcgaag
    12841 aaagcagttt caaattgtct ccaaaggaag ctcatctgct ggcgttgttc tgtaagcgaa
    12901 tcaataatgt actgccaagg tccgaagcat taagccgcat ctggggcgaa gacaattatt
    12961 ttaccgctcg aagtatggat gtgttcgtta ccaaactgcg aaaatacctg aaactcgacc
    13021 cgaagattga aatagtcaat atccacggta atggatttca attgtttatc agataagaaa
    13081 gtaacccttt tccttatttg acaatattct ttcgctcgcc atctttatga agggtgacaa
    13141 atgcatcttt aaagcctaat ctttttaccg aagcaagtgt tttgatggca tctttccggt
    13201 cagcccacca tgcaagcaat acacgttcca tctcctgatt gggtaccggc tcgtgttcga
    13261 ttgtaccgta ttgcttggct ttctcaaatg ctccgctcat aatgtcagga taccgtgcag
    13321 cggccagctg aatgagataa tactcgcctt ctttaatgaa tgacggcgga cggtgtacat
    13381 aaggctcttc agcttttggt tcgggttttg ccggctttgg ctcttgcttt ggtttctctt
    13441 taaccggtgt tttcttttct gttttcttaa tgttttcagt tttgggctgt tggatcaatg
    13501 ttggttcagt gcctgcaata gaagcttctt tttctttttc gagtgaaaaa ttttgcgtga
    13561 gtttgttggc ctttacatct gaggtggtaa agcggaagaa tgcgggtgca tattcaaatt
    13621 tttcaacttt tgccctgtaa ctcttggaag catccaatgc aaagctaaat gtaccatcag
    13681 gcgaatgccg gttatctacc aagctctcac tcccatccgg gaataactgg taaaccgcta
    13741 tccgcacatc agtaagtaag ctatctgttt gggccttaaa tattttgccc gtaaacagat
    13801 tttgaaaatt ggtgtactca aacctgaaaa tatcttcatt atccgtggtt ttcttgaggt
    13861 caccaaatat ccggttcgat accagatagc cggaattgag atcaccactt ttgatgaaat
    13921 aaaaatcatc agccggactg ttgaccggaa aacccaagtt tacggtttct ttccattctt
    13981 ttccaaatcc catattgctg catacatcga gccctccggc acctatctgt ccgttagagc
    14041 tgtagtatag ttttccttcg taaatgtcgt accatggtgt tacttcatcc ataaatgtat
    14101 tgacctttgg gccgcagttt ctgggcactg taaagtcaag atcatcggtg ctaaggtcgc
    14161 gctcacagta ccaaatgtcc tggccaccat agccgccact tctgtcagag ctgaagtaca
    14221 gaacttcctt gtttgcaaat ttgcagaccg agggctgtgt cgttgtagcg accgaatcat
    14281 ttatatagtc acgcagtttt attggttcgc cccatttgtc gccttttcgc atgatgacgt
    14341 atatatcaca ttttgcccga agaccgtctt tcgtctctac ttctttgcac cttgtgtagt
    14401 aaaacctttt aaaatcataa ctgaatgagc cgttaccgaa atgctctttt tcatcttttg
    14461 gaaaaatggg aggaaccgtc ggcttagacc aggaagatcc gtcccattcc gatttgtaaa
    14521 ttttcgagct gcctttccga tttgagctga agtatagcac attctcgccg tatggtacag
    14581 gtgcatattc ggtaaagcct gtgtttatta ccataccagg ataagatatt ttataagctg
    14641 atgtagtcga atcactcagc attctcaaag cctgtttgct tccttctatt tcgcggttta
    14701 caagttcttg ctgttgtggc cggtcatcac cgttataagc tttcatgtaa gattcatacg
    14761 ccttgattga tttttcgtaa gctccacttt gcttcagggc attggcgtat tctatggtag
    14821 ataccggata tttcgacgac ttttttgata ctttttcgaa tgcattggcg gcctctttgt
    14881 agtttttaac caaatagtaa gcctggcctg ccttgtataa gagatcggtc ttacttttct
    14941 tttttagcca tgctgccttg taatttttag cggcagtacc ataatcaccg gctttgaagc
    15001 ttttttctgc tatgtccgaa tagtctctcc acgacatttt ctgaccaagt gacagttggg
    15061 ctatgcctgt aagtagtatt aaagtgaaga aaaattgttt catggattat gttttgtgca
    15121 gattatcttt ttggttaaat gaatggaaga tcagttgagt agttgtccta ttacttttgg
    15181 gtaaaattcg tattccaact tgtgtacttt tgctgctata gtatcgggtg tatcatcgtc
    15241 cgagagactg caagacgtct ggaaaatgat atcgccttca tcataatgcg gattgacata
    15301 atggatggtg atacctgttt cttgtttatt tgctgccttt acggcctggt gcacacgcgc
    15361 tccgtacata cctttaccac cgaatgccgg taacaaggca ggatgaatgt tgattatttt
    15421 cttgggaaat gactggatga acaattcggg taccagccag aggaagcctg ccaatactac
    15481 caaatctatc tgattgtcaa gtaaataccg aattacttta tctgtttcgt aaaattcttc
    15541 cctcgtaaaa accaaagaat caacgccggc ttcctttgct acatttagta cacctgcagt
    15601 tggattattg gataaaactg ccttgacact tatgtcatga ttgtcatcaa aataccgtat
    15661 gatgttggcg gcattggtgc ctgttcctga agcaaatatt gctatacgtt tcaagctaga
    15721 ttatttagtg gttattaagg tgcggtaaaa ttaacaataa attataaaac tttataatat
    15781 aaggctttta ttcatcagaa aatgagcttc atagttagaa aattgttaaa cctgccttac
    15841 cagaatcata aaaagtggct ttttttgttt tctacacgta tatttgaaga ataatttttt
    15901 tatgacacac caaaagggct taccaatgaa attcaaaaac ccggttctgt tttcatttat
    15961 tattggcgca tttgcattat tcgctttttt gccaaaggct gatgttgacc caacacaaaa
    16021 ggaaactgta ttgctgcaaa cgctaataca agggctagaa caactgcact acaacccacg
    16081 tacattggac gattcttttt ctcaagacct atttaatctg tacattgagc gccttgatgg
    16141 tgccaaacgg tttctcactc aagcagaagt agataagctg aaacctttcg aaaaacaatt
    16201 ggatgatcag ataaaacgaa ccgatgtgag cttttttaac acatcaaccg acttgttgac
    16261 caacggtatt aatagggctg aaaagatata taaagaaata cttgaaaagc catttgattt
    16321 taaacaaaat gaaagtatag aaaccgatgc cgaaaagcga aaatttccgg ctaatgaaaa
    16381 ggaattgaaa gaagtatggc gcaaaatgct taagtacgaa acaatgaccc gattagcgga
    16441 caaactcgac gaaaaagcca aaggtactgc agaatataaa gacaagtctg aagctcagct
    16501 tgaagatgat gcccgaaaaa tcgtacgtga acgattcgaa acttatttcg accagctaaa
    16561 aaaactgaaa agagaggagc agtttgctga tttcctcaat actattgcta acctctacga
    16621 tcctcattct gaatattatg agccggtcga aaaacaatca ttcgatatta aattctccgg
    16681 taggctcgaa ggcataggtg ctacattgca aaccgataaa gaatttacaa aaatagcaga
    16741 actcgtcgta ggcgggccag cgtggaaaca aaaggaactg aaggtaggtg atctgatcat
    16801 gcgggtggca cagggtgacg aagaagggat aagcattgca ggtatgaatc tgaatgaagt
    16861 agttgccaaa atacgtggcc caaaaggtac aaaggtacgt ctcacagtta agtccatcga
    16921 tggtactact aaggaaatat ccatcatacg cgatgaagtt attattgatg aaggttatgc
    16981 caaatcagtc atactcaaca aagaaggtgc cgagaatgtc gggttcatta gcttgccaag
    17041 gttctatgcc gactttaata aacgcgatgg acggcagtgt tttcaggatg tggcaaaaga
    17101 aatcgaaaaa ctgaaggcgg caaatgtaaa tggaatcatt attgatcttc gaaacaatgg
    17161 tggcggttca ttgaatgatg tggtaaaaat gaccggattg tttatcgaaa agggtccgat
    17221 cgtgcaggtg aaaacacgct tgggtgacgc agaggttcag caagactatg atagccgggt
    17281 acagtatggt ggtccactta tcattatggt caataacttc agcgcatcgg cctccgaaat
    17341 tatggctgct gcattgcagg attacagcag ggcagtaata gtaggcagca accatacttt
    17401 tggcaaaggg acagttcaac gcttcttcga cctggataac gccgtcaatg gttatgacaa
    17461 tcttaagcct cttggcgatg taaaaataac cctacagaaa ttttatagaa taaacggggg
    17521 ttctacccaa ttgcgcggcg taagctccga tattgtactg ccggataatt ttcaagacct
    17581 gaaactcgga gaaaaagaat caaagtatgc catgccgtgg tcgcagattg ataaactcaa
    17641 ttatagccag aatgtttaca acattggtaa tgtcagcgaa ctcgcagcgc aaagcaaaga
    17701 aagagtagga agcaatgaaa tttttagtaa aatagcagcg aatgcaaaaa gacttaaaaa
    17761 acaacgtgac cagtctgttt acagtctgaa tatcgaagaa taccaaaatc tcaagaaaaa
    17821 tattgaaaaa gaatcggagc agataaagaa gctttcagag acagaaatac ccgggcttgc
    17881 agtgagcaat ctgaatgttg acctccagag tatcaacagc gattcgacaa aaatcaaccg
    17941 caatgctgaa tttctgaaag acatgaaaaa agacatttac gtggacgaaa cactcaacat
    18001 tatggccgat ttgatccgca agaatcttgc atccaagaat taaaagctaa aacaaagtat
    18061 atttgcaatt ggttgccggc aacaccggtg gccaattgtc atttatagca atacccgatt
    18121 ttaaacaata caataaaatt ttatgaatta cctgacactc gagcatgtta cgaagaccta
    18181 tggcgaaaaa accctgttcg aagacattaa cctgtctgtc aataaaggcg ataaaatagc
    18241 tctggtggcc aagaacggtt caggtaagac aacattgcta cgcgtaatag ccggtgaaga
    18301 atcaggtgaa ggcgaaaaat gctccatcca gctacacaaa gatgtcaaaa cgcggtttct
    18361 cagccaggaa cctgaatttc atgaaggtga tactgtcata gaagcattac tgtcaggtga
    18421 cagtgaacag tcaagagtga tacgccgcta cgaaatattg cttgataacc cgggtgacgg
    18481 taccgagctg caggcggtga tggcaagaat ggacgaccta aaggcttggg attacgaatc
    18541 gcgtgtgaaa gagattctgc acagatttaa tattcgtgat tttgacaaac cggtagcact
    18601 gctttccggt gggcaaaaga aaagagtagc gttggcaaga ctcatcatcg aagaacccga
    18661 attcatcatt cttgacgagc ccaccaatca cctcgacctc gatatgatag aatggctcga
    18721 agattacctg aagcagccgg gtattacgtt gttcatggtc acgcacgacc gatattttct
    18781 cgaccgtgtc tgtaatgcca tagtagaact cgatgccgga catattcatc gttattcggg
    18841 caattacgaa gcatttcttg aaaaaaaagc tgagagagaa gaaaatgaag ccgttgtgct
    18901 gggcaaaacc aaacaattgt tcaaaaagga actcgaatgg atgcgccgtc agcccaaggc
    18961 acgcactact aaagccaagt cgcgtattga cgaatttgaa gtcattaacg aaaaagcaca
    19021 tgtaagaaag gacgactcaa gagtacagat cgatctgcag tcgaaccgtc ttggaagcaa
    19081 aatccttgag ctccactaca tcagtaagac gtatggcaag cagaagctca ttgaagattt
    19141 cttctacaaa tttaacaaaa aagaccgagt aggtatcgtt ggacctaacg gcgtgggcaa
    19201 atctaccttc ctgcgtctca taaccagtca agaaagaatc gatgccggaa aagtaataac
    19261 aggtgaaact actgtattcg ggtattatac acaggacggg ctcaacctga aatctgacaa
    19321 gcgagtgata gatgtcatac aggatatagc cgagtatgta ccacttgaga aaggaaagaa
    19381 aatgtcggca gcacagttgt tggagcgttt tctgttttca agagcacaac agcaggtata
    19441 tgtgtcgcag ctgagcggag gtgagcgccg caggctatac ctccttacca tactgatgca
    19501 gaatccgaat tttctgattc tcgatgagcc tacaaacgac ctggatatac tgacacttaa
    19561 cgtactcgaa gaatatctgg aggagttccc gggctgcctg atagtggtca cgcacgaccg
    19621 gtattttctg gacaaaatcg tggaccacct ctttgttttt gaaggtaatg gcaaaatcaa
    19681 agactacaat ggcaagtatt ctgactacag atgggaagat aagcaacgta aaaaggagga
    19741 aaaggcagag aagtctccgg aacaaaagcc tgcaatcaag cccaccggca tgctgagcaa
    19801 tgaagagcga aaagaaatga tgcggttgga gcgcgatatc gagaagctcg aagcagagaa
    19861 actcgcaatt acagaaaagt tcaatgatac cagcatgcct cttgaagaaa tcacccaatt
    19921 agccaataaa ctcgaagagc taaaaaccct gcaggaaaca aaagaaatgc gctggatgga
    19981 actttgtgaa aaggctgggt gagtagagaa gtttgtgcgg gagtgatgaa atcaacagac
    20041 cgcaattttt ttagatgatg cttgcttttt gttaaaatgt tatgtatgtt tgtcaaataa
    20101 agcagattaa aaaccaaaaa ctcaagattg tatgaaaaca caaccaaatt atcaaaaaga
    20161 atttcgtttt ttacgaaata ttattctgtt taacgggggg gtaaaatttt attttccacc
    20221 taggtggcag ctgatattga tttttgctat gctgttcatt gttaaccctc ggctattatt
    20281 cagtcaatgt gagacaccag acccatcgga tgagttattt gaatattatg tttcgttatt
    20341 accttcccaa aacaattaca ggcttgcacg agcttcaggg gatccggcaa gcataccggt
    20401 ttcatttacg gtagtccgga atacggaagg aagctccaca gatgtttccg tatctcagat
    20461 tgatttgacc attgatacac tgaataaacg ttttgctcct gtcggaataa gtttttatcg
    20521 tttggggaat gttaattata tagattggac accattatat ttaaatagca ttattgatgg
    20581 tgcacgatat tcttatttaa gcacagcaat taatgtcatg gtacatcctt tacaggccag
    20641 tgtagctact cagccaataa tgactgataa tcctgattat tataccttag aggacaatat
    20701 aatatgggta gaacccagcg cattatgggg aaatttcgct agcttttccg ctgcttttat
    20761 acatgaagtc ggtcatatat ttggactatt gcacacacat ggtgcgacta cggtatatca
    20821 ttcaccgcct gacagtgctc aagtagatta tccttatccg aatgaaaaaa acgataatag
    20881 taaatatggc cgggaattac aaataaggga cacgacttac ggtatagcct tcccgtaccc
    20941 aaattggatg gtagccggtg atttatgtgg cgacacatat ccttcggtaa tgaataataa
    21001 tctttatttt cccgggtgga gcatagattg tccatataac gggacttatg ttgattacaa
    21061 caatgatccg gtaacgcccc aggagaaaaa tatcatgagc tattttattt tgaatggatg
    21121 t
//
LOCUS       DAOQQY010000020        20697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_768_length_20697_cov_6.535171, whole genome shotgun sequence.
ACCESSION   DAOQQY010000020 DAOQQY010000000
VERSION     DAOQQY010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 20697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20697
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_768_length_20697_cov_6.535171"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..633)
                     /locus_tag="PLE23_02205"
     CDS             complement(64..633)
                     /locus_tag="PLE23_02205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX82107.1"
                     /translation="MKNLESSTDEELVKLYKTIQDPAIIGALYKRYSHLVLGTCIKHL
                     NDTELAKDETMMIFEKLPLNILNHDVHRFNHWIYSITYNACISRLRKKQSEQRRIGHL
                     SDEVAELVDDTDYETIRENELKANKVHWAVRQLANEQATCISLFFFEHKSYKEIAQIT
                     GMQESTVKSHLQNGKRNLKNLLSTKTHDE"
     gene            complement(826..1065)
                     /locus_tag="PLE23_02210"
     CDS             complement(826..1065)
                     /locus_tag="PLE23_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82108.1"
                     /translation="MEQKSTYYRTLLRYIYQETADSDVQFMAEEIYNNRSMQKEYNEL
                     MGCITHLPKVKMSPERTSVQNILHYMRMSSLEAQH"
     gene            complement(1148..2263)
                     /locus_tag="PLE23_02215"
     CDS             complement(1148..2263)
                     /locus_tag="PLE23_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HMX82109.1"
                     /translation="MSTTKARPKILVAPLNWGLGHATRCIPVIKELINQDAKVILASD
                     GRSYELLRSEFPELKLYRLTPYDVMYSSDSMIFNIAAQLPKILLAIYKEHKSFARIIQ
                     QEKIDGIISDNRFGCYHSSVYSVYITHQLNIKIPLKPVEMFTRMVNRKIIDRYDECWI
                     PDYAGKDNISGELSKPEKIKNAKFIGTLSRFEARTLPKKYDILAILSGPEPQRSNFEK
                     LVLEQLKELPLKCAVVQGKTEVVERISDGNLDIFSHCSVPTLNDLIASSDVVIARSGY
                     STIMDLIQMKKRALLIPTPGQTEQEYLAEKLQKRAWFMSQEQKNLDVAKAIEQIPEFD
                     ADLFEKYKDDNILSETISAFLKNCEIKNKKLIKKIKT"
     gene            complement(2278..3828)
                     /locus_tag="PLE23_02220"
     CDS             complement(2278..3828)
                     /locus_tag="PLE23_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HMX82110.1"
                     /translation="MEKELIINVSPNEVEIALLEDSKLVELHHQKTNSNFAVGDIFLG
                     RIKKLNPSLNAAFVDIGYEKDAFLHYTDLGPRLNSLLAYTENSLNGNQNTSRLDHFTI
                     EPEIIKNDKIENVFSKRKAVLVQMLKEPISTKGPRLSCEIALPGRYMVLSPFSDIISI
                     SKKIDNPEERKRLQVLVESIKPKNFGVIIRTAAEGKKVADLHEEMKAIVSKWEHIHSQ
                     LYNAVPPLKLLSELDKSSSLIRDLLTDSFNKIVVNDKELYFSIKSYLESFAPAQLKIL
                     TLYKDSRNILDEYGVTHQIKASFGKTFTLSSGAYIIIEHTEAMHVIDVNSGHKVSTTD
                     QEESIMRVNLEAAEEIARQIRLRDIGGLIVIDFIDMRNVDHKRILMEKMRDFMEKDRA
                     QHTILPLSKFGLMQITRERVRPQVVINTTEVCPTCNGSGKINASVLVTDDIVRDLEFI
                     LHSKPKKAVYLHVHPFVEAYLKKGLPSMQMKWFMKYNKWIKIRSDVNNALNEYRFFDD
                     NNDEIRFS"
     gene            complement(4146..4931)
                     /locus_tag="PLE23_02225"
     CDS             complement(4146..4931)
                     /locus_tag="PLE23_02225"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82111.1"
                     /translation="MSKNQIIAVSSAVLLTFVLYFGFGEVPKAEPPAATKTKEDKTYE
                     TVLLASKEEATAKLTPEQKNTLHTLESVLSSTKNDSTKVESLKQLASFWYQSGKPEVS
                     GYYADMVSDILKDKEAYQITGTTYRIALSEGGLDEKVRDVCSRRAIRAFEKAIRLDST
                     NVNYQMQLAMVYVDKPLEDNPMKGILMLRELNTKHPENADVNIQLGRLAIKTGQFEKA
                     VERLKKAVELSPGTVSIYCLLADAYTGLGDKTNAGLAEKKCKK"
     gene            complement(4947..5261)
                     /locus_tag="PLE23_02230"
     CDS             complement(4947..5261)
                     /locus_tag="PLE23_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMX82112.1"
                     /translation="MRKADLVSIISDKTGVPKVDVLVSLETFFKEVKATLAKGENVYI
                     RGFGSFVIKRRAKKIGRHIKQNFAIEIPEHFIPSFKAAKMFVDQVKDNVTSMPEDIEE
                     ED"
     gene            5477..6340
                     /locus_tag="PLE23_02235"
     CDS             5477..6340
                     /locus_tag="PLE23_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HMX82113.1"
                     /translation="MTGFGSARATYGPKNITVEIRSVNSKFTDVKFKLPQNYRDKEFD
                     IKKILTDDVTRGKVEFNLSIQSNDGPAQVSINKNLFRKYYNELTLLAQELNMPTGDIM
                     QAILRIPEVINADEQTIPDEEWELVRKTISEAVATFQNYRLAEGESIKKDLIARIDSI
                     QQLHNKIDPFATSRIQNLRIRLKQLLDEHSLSDMVDGHRFEQEILFYMEKIDINEEKV
                     RLTQHCKYFLQELDSDDIQVGRKLGFISQEIGREINTLGAKAYDANIQRLVVMMKDEL
                     EKIKEHVANIV"
     gene            6344..6910
                     /gene="gmk"
                     /locus_tag="PLE23_02240"
     CDS             6344..6910
                     /gene="gmk"
                     /locus_tag="PLE23_02240"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMX82114.1"
                     /translation="MNKMIIITAPSGAGKTTIVKHLLKVFPTLSFSVSATTRAPRPHE
                     SNGKEYYFISTDEFQQKIRNHEFIEYEQVYENQYYGTLHAEVQRLWSEGRSIIFDIDV
                     KGALNLKKIFMNEAFSIFLKPPSFEILKERLLNRKTEDKASLNKRIKKAKLELKYQDK
                     FDYVLINDKLEISLAEAENLVRTFLEIQ"
     gene            6950..7345
                     /gene="apaG"
                     /locus_tag="PLE23_02245"
     CDS             6950..7345
                     /gene="apaG"
                     /locus_tag="PLE23_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HMX82115.1"
                     /translation="MGSIQSLVTKGIKISVESFFQSEYSNAIENKYIFTYKVKIENNN
                     SFAVKLLRRHWYIFDSNGLFREVEGEGVIGQQPMIESGDEHTYVSWSQLETDMGQMNG
                     KYTMLNLNDNSAFEVEIPFFQLVMPSKLN"
     gene            complement(7437..14666)
                     /gene="sprA"
                     /locus_tag="PLE23_02250"
     CDS             complement(7437..14666)
                     /gene="sprA"
                     /locus_tag="PLE23_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HMX82116.1"
                     /translation="MNYKQLIISFLLTVLVGVFSFAMLNLPQIPRVAYHHPAGSTSSE
                     VTDTIPEKKTEYPLYKPKTLIDLKDPASVEKSEEYDPASGQYILSEKIGNEYYKAPVK
                     MSFDEYLNYKSKQQEREYFDYLSGVSKSSGKKYDPKNPLARYIPNKSLLNRLFGGTEP
                     VINPTGNIDLTFGYDYSKVENPTYPIRNQTTGGFDFDMNIQMNVTGKIGEKLNLSTQF
                     NNRATFDFENQMKIDYNSTKFNEDEIIRSIEAGNVSLPLKSSLIKGNNSLFGVKTELQ
                     FGNLRLTAVASQQKSRRKAIELKGGKELQTFAVKADEYDENRHFFLSHYNRNNFERSL
                     ENLPQINSLFRLNKIEVWVTNDRNVTEKVRDIVAITDLGESERITNSNPQFQKPAGIR
                     NPDIYGKELPANSSNDIYESLITRPDVKTINKAVATLTGPEYKFSQIRDFEKVRARLL
                     NDNEYTINNELGIISMNINMKPSDVLGVAYEYTYNGKTFRVGQLSTENPNNPDTLGVL
                     FVKMLKSTTSRVDLPMWDLMAKNFYSIGAYNVEQEDFKLDIYYEDPGGGEKRFLPSSN
                     LAGEPLIQLFNLDKLNKRNDPFPDGIFDFVPGLTVNTRSGRIMFPVLEPFGSYLGDKI
                     DNANLRKKYGYYQLYDSTVTQAREFPELNRFTLKGSFKAGTTSDISLGVFNLPQGSVT
                     VTAGGVTLTPNVDYSVDYNTGRVKILNDSYLQSGVPINISFEDNSLFSFQTRTMLGLR
                     ADYKLGKDINVGGTFLNLFERPFTQKVNIGEDPINNKIYGADITLKKDLPWLTKAIDK
                     IPLIQTKEMSAINFSAEAAALKPGHASAVNESAEKGGNVYVDDFEGSTNGFDIRQPAT
                     NWVLASVPQMAGNDKKELFPESSLIDDLRSGVNRAKLAWYRIDETVRATSAGADNNYV
                     TAIPIKELFPNRQIPTGYNTNIQTFDLHYEPTVKGPYNFDLPLGTTYSKGIEKNASLK
                     EPATRWGGIMRNLQNNNDFEAANYEYIEFWLLSPFIDNSANPGDLYINIGNVSEDILR
                     DSRLFSENALPNVKNPNLKVDRTVWSNVPRIPPINNSFDADPEVRKQQDIGLDGANDD
                     DERVKFADILNQYQGYLTPEAMNEAMKDPSNDNFISFQDPVWNSSSTVYDRYSRYNGT
                     EGNNQPAKDGLINSSTQNPDSEDVILDNTLNEAESYFEYKIPLVPDGNVGIENNPYVV
                     EKIDGPAGSNRTWYRFVVPIQEYSRKVGGIQDFRSIRFIRMYLTGFNQEVTLRFATLE
                     LIRSQWRRYKRPFSSVIEVDPDNKTSFDVKSVSIEENSQKLPFNYVTPPGIIRENTLS
                     NAQSLLQNEQSIAINVCNLKPGDSRAIYKLSGMDLRVFERMKMFVHAESQEKIAPGGL
                     SVFLRMGSDFQNNYYEYEIPIELSDPDNTYTPGTPEYEAEVWRKANNLDILLKLFKEA
                     KQERNKSGISLIDVFEMADPDFPSNKVRVIGNPNLGLVKGFMVGVKNNDKIPHCAELW
                     VNELRVNGFDENSGYAALARAEVKLADLGRVNVAGNFTSIGWGNLEQRLAERQREQVV
                     QYDGAVELELSKFLPPNTGLKIPFLAQVSNTTRTPEYDPYDLDIPLKEKLDNIDDKNV
                     RDSVRSSAEDYTNIKGFNFTNVRKERTGTAVKPKPWDVENFAASYAYTQTDHHDPIIS
                     SDRIKNYKGALDYNFTRQVKYVEPLKKVLKDAAWLKLLSDFNFNPIPNGFVLRNAMDR
                     TLQATSYRFAGTDPTKNTFYDKRWSWDRNYSLNWDLTRSLKLTFSANNQSLIDERPEN
                     TINPETNVFYTDAEKSKYIKDNLKKFGRTKTYSHNIGVNYTLPTKGLLITDWTTIRAN
                     VNSSYSWNTASLNTDSLGNVIQNTQQRQLNADLDFEALYNKIKYLKKINKKEAPKRSV
                     DARGVMKDPGKPEEKKNAKDPDLKDTKTDKKDKKDREPSIIEKIVIRPLMMVRKGRLT
                     YSESYTNVVPGFTPKTNLLGMNQGFTAPGLPFILGERPTDRWFDESADKGWFTESVFL
                     NQPVSRNYTRDISGQIKLEPFNDFKLDVDLKQNYALSRQEDFKKPSVTEDFQHLSVRE
                     VGSYTISYLAINTLFDKNVNGLFKKFEDNREIISQRLGTGKHVTDYLYGNYTEGYGRY
                     QQEVLVPAFIAAYSGDNPSKVVLNEKLFSKLPLPNWKLSYGGLNKLPLLKDIFSSVNI
                     THGYTSTLTISNFNTNAFYNPNDPLENLQPITNNYFSRYEIPAIVITEQLSPLLGVDV
                     KLKNDMSARVDMKKSRNLQMSFVDNTLFETNTTEYVFGFGYRIKNIDIPFLVKKAPVK
                     KKTDDKKLPSSKKGTTNTGRDLNIKVDFSYRDDLTTNHLLDQDISNPTRGSTTIRITP
                     NADYQLNKRLNLRLFWEYSRILPKTTESFPTTNTSAGITVRFSLK"
     gene            complement(14819..15409)
                     /gene="ruvA"
                     /locus_tag="PLE23_02255"
     CDS             complement(14819..15409)
                     /gene="ruvA"
                     /locus_tag="PLE23_02255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HMX82117.1"
                     /translation="MIAYIKGPIAYKSPTFIIIDIQGIGYHINISLHTFSKLEQLSEV
                     KLATHLMIKEDSHSLYGFFEESEKILFLQLISVSGIGASTAQLVLSALTPDQIKAAII
                     QEDDKTFSRVKGIGPKTAKRIILDLKDKISKDSSNVNINLVVSDNTIKDEALFALLAL
                     GFMRPAASQVLNKILLEEPGISSVEVLIKKALGRLS"
     gene            complement(15497..16261)
                     /locus_tag="PLE23_02260"
     CDS             complement(15497..16261)
                     /locus_tag="PLE23_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX82118.1"
                     /translation="MKATLLGTGTSQGVPVIGCRCEVCMSPNPHDSRFRTSALITDGK
                     TNVLIDCGPDMRQQMLRIGIAHLDAILLTHEHNDHIIGLDEIRPFNFLQNMIMPFWAT
                     DRVKAELYRRFSFAFEEEPYPGAPRAKVEPLEKNKSVTIGGINILPIEASHGQMPVMA
                     FRVGDFTYMTDVKFIEPDQIDLIKGTRYLVINALRREEHHSHLSLSEALQLIEQINPE
                     MAYLTHISHHLGTEANLNKELPANVKLGYDGMEIIW"
     gene            complement(16258..16542)
                     /locus_tag="PLE23_02265"
     CDS             complement(16258..16542)
                     /locus_tag="PLE23_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82119.1"
                     /translation="MIKYTKHFLNKLEDFLGKQEYDVRYEKGNFQSGYCLVESNKVVV
                     INKFFDTEGRINNLIEIILQIDIDEEKLDTKDKDLLKHLIHSWESSNTEA"
     gene            16630..17214
                     /locus_tag="PLE23_02270"
     CDS             16630..17214
                     /locus_tag="PLE23_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMX82120.1"
                     /translation="MLFIISHNLHAQTFGATIQAGLVASQIDGDQYAGYNKPGPFIGI
                     NGIARLKEKFYIETGIAYVFRGSQSEFLQDNSIPQFKINLQYIDIPVTFHYKDWLVPE
                     DEFYRMDFYAGGYFGRLIHSYAEYSGYDDVLDLFNKNELGIVIGGAYSFGPHGSLGAK
                     WCRGLNYLFKHNTNGVNQNSLINRFIQFYYQYKF"
     gene            17217..17759
                     /locus_tag="PLE23_02275"
     CDS             17217..17759
                     /locus_tag="PLE23_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82121.1"
                     /translation="MRNVFFVTMLCLAVLVACKNETATQTTLTTAQPENAPTSTGITA
                     GPAATLPIIPLEEYKALAENTNSIDIVPFESNLSMSIPDKASCYQFVANHILDTQVSE
                     MPCTKPNGRIFFNKDGDSILEANIYYTEQCKYLVFYKAGNNTPLYATQITEQGKKYFE
                     SIYGANAMQELQKLQSGNQK"
     gene            18002..20278
                     /locus_tag="PLE23_02280"
     CDS             18002..20278
                     /locus_tag="PLE23_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HMX82122.1"
                     /translation="MNKQLKTKGHQFFLFLALLLSLLACKNNTQENSAESNTVNGETL
                     GTILDKLTEQYYNPDNTYAAGARLAHYDSLLKVTQDIQSSLNYAVLHANTLLEYGKEK
                     RAVAELEKIYDYVKSVPASRLAAGEMLGVAYMRLAERTNCIARHNADACILPIKGLGI
                     HQDKGPARKAVEIFEKILKEDPKMLNSRWLLNIAYMTLGEYPNGVPKEFLIPGIGQGE
                     TTTVKPFTEMASDLGIALYKQSGGTIVDDFDNDGNLDIVVTGWSLKETLHYYHNNGDG
                     SFSDFSERSGLNKIRGGLNMMQTDYNNDGKIDIFLLRGAWQGSGPFAKVPNSLLKNNG
                     DGTFTDVTIQAGILSFNPTQAATWNDFNNDGWLDVFIGNESLPSNNIYPSEFYINNQD
                     GTFTNIASPQGLNIKTFVKACTSGDYDNDGWADIFISCLTGEKILLRNKGVKGKVPTF
                     ENTSQQAGLAQNKERTFPTFFFDYDNDGWLDLFICNYSFEQQLSFFLAKETLYPSNDQ
                     EGKIILYHNNQNGTFSEVSGQMHLNQPVFAMAANFGDFDNDGWLDMFMSTGNPNYRSL
                     IPNRLYKNMGGTDFMEVTYSARVGNLQKGHGVAFADINNNGDQDIYVNIGGAYEGDAF
                     NSAFYLNPGQNDNNWITMQLEGVKSNKAAIGARIRLKFTDNGKQRFVYRDLNSGGSFG
                     SSPLRREIGLGQAKMIDEIQIKWPASGTVQTITNIKPNQFIKITEGKEGYSTINLKNI
                     VFKYKSGGIPMCAPRVSI"
     gene            complement(20313..20696)
                     /gene="rbfA"
                     /locus_tag="PLE23_02285"
     CDS             complement(20313..20696)
                     /gene="rbfA"
                     /locus_tag="PLE23_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HMX82123.1"
                     /translation="MRQKQIGEIIRRNVSIVLSSEGSYIYGDALVTVTNVMVSPDMSQ
                     AKVYVSVYNTEEKQAVVLMLEDQLVRIRQALAHRIKKHVRRIPEIYFYLDDTLDEMYR
                     LNNLFDKLEAEDQLGHNRPPDDDDE"
BASE COUNT         5996 a         4251 c         3858 g         6592 t
ORIGIN      
        1 aaactacctg ttagatattc agtgatttct tcacgggaca aacaaggtct tacatcaaaa
       61 attttactca tcatgtgttt ttgtggacaa tagatttttt aggttccgtt taccattctg
      121 taggtgactt ttaaccgtac tttcctgcat tccggtaatt tgagcaattt ccttgtagct
      181 tttatgttcg aaaaaaaata gacttataca agtagcctgc tcatttgcta attgtctcac
      241 ggcccaatgg accttatttg ctttgagttc gttttctctt attgtttcat aatccgtatc
      301 atcaacaagt tctgcaactt catcagacaa atgaccaata cgacgttgct ccgactgctt
      361 tttccttagc ctactgatgc acgcattgta agtgatactg tagatccaat ggttgaatct
      421 atgcacatca tggtttaaga tattgagtgg caatttttca aaaatcatca tagtttcatc
      481 tttagccaac tctgtatcat tcaagtgctt aatacatgta cccaagacca gatgcgaata
      541 ccttttgtac aatgcaccaa taatagcagg atcttgtatc gttttataca attttaccaa
      601 ttcctcatca gtagatgact ccagattctt cattttctat aagtgtaatt taaagacgct
      661 tagtgtgccc aaattccata atattttcca aaaatttttt gtttttagat gaaatttcac
      721 aaaataggct gaaaataccc tatgcaaagt aaatacaata ataaaaaagc ctgctacgaa
      781 aatcgtgcag gctatttttt tatctactaa aatatttaag acaaattaat gctgtgcctc
      841 aagtgatgac attctcatgt aatgaagaat attctgcacc gaggtccgtt ccgggctcat
      901 ttttacctta ggcaaatgtg tgatacagcc cataagttca ttatattcct tttgcatact
      961 gcggttatta tatatttctt cagccataaa ctgtacatct gagtcggcag tttcctgata
     1021 aatatagcgt agtaatgttc tgtaatatgt agatttttgc tccattaaga aataaatttg
     1081 aaattattaa taaggtggtc ttctggagtt ataactgaaa tgtcacgctg tttattgtac
     1141 tgaataccta tgttttaatc ttttttatta atttcttatt ttttatttca caattcttta
     1201 aaaaagcgct gatggtttct gataaaatat tatcgtcttt atatttttca aataaatcag
     1261 catcaaactc aggtatctgt tcaatggctt ttgccacatc caggttcttt tgctcctgac
     1321 tcataaacca agctcttttc tgcaattttt cagccagata ttcttgctca gtctggcccg
     1381 gtgttggaat aagtaatgcc ctttttttca tttggatgag atccataatg gtagaataac
     1441 cggagcgtgc aatgacaaca tcactcgatg ctatcagatc gttcagggtt ggaacactgc
     1501 aatgtgaaaa aatgtctaaa tttccgtcag atattctttc taccacttct gttttacctt
     1561 gcactactgc acactttaag ggaagttcct tcaattgttc gagcaccagt ttttcaaaat
     1621 tggaacgctg aggctctggc ccggataata tggcaagtat atcatatttt tttggtaatg
     1681 tccttgcttc aaaccgactg agagtaccta taaacttagc gtttttaatt ttttcaggct
     1741 ttgataactc tccactgatg ttgtccttac ctgcatagtc gggtatccaa cattcatcgt
     1801 atctgtcaat aatcttccta ttgaccattc gtgtaaacat ctcaacaggt ttaagcggaa
     1861 tcttaatatt caactgatgc gtgatgtaca ccgaataaac actcgaatga taacaaccga
     1921 atctattatc agaaatgata ccatctatct tttcctgttg gataatcctt gcaaatgatt
     1981 tatgttcctt ataaatggct agtaatatct ttggcagctg agctgcaata ttaaaaatca
     2041 tagagtcact tgaatacatg acatcataag gtgtcagccg gtaaagcttc agttcaggaa
     2101 attcagaccg aagcaattca tagctcctgc cgtctgaggc gagtattact ttagcatcct
     2161 ggttgataag ttctttgatg acaggaatgc atcgtgttgc atgacctaag ccccaattga
     2221 gtggggcgac caatattttc ggccttgctt tcgttgttga catacttttt gtatgtatta
     2281 tgaaaatctt atctcatcat tattgtcatc aaagaatctg tattcattca atgcgttgtt
     2341 aacatccgac ctgattttta tccatttatt gtatttcata aaccacttca tctgcatact
     2401 tggtaagcct ttcttcaggt atgcctccac gaacggatgt acatgcagat aaacagcttt
     2461 cttaggtttt gaatgtaaaa taaactcgag atcgcgtaca atatcatctg tcaccaatac
     2521 cgaagcatta atctttccgg agccattgca agtagggcaa acttccgttg tgttgataac
     2581 cacctgtggg cgtacccgct ctcgtgttat ctgcatgaga ccaaacttcg acaatggcaa
     2641 gatggtatgc tgtgcgcggt ccttttccat aaaatcgcgc attttttcca tcagtatccg
     2701 cttatggtca acattgcgca tgtctataaa atcaatgact attaacccac cgatgtcgcg
     2761 gagtcttatc tggcgcgcta tctcttcagc agcttcaaga ttgacacgca tgatagattc
     2821 ttcctggtca gtagttgaca ctttgtggcc actattcaca tcaataacgt gcatagcttc
     2881 agtatgctct atgataatat atgcaccact tgacaaggta aaggtcttac cgaatgaagc
     2941 ctttatctgg tgggtgactc catactcatc aaggatattg cgcgaatctt tatataaagt
     3001 caggatcttg agctgagccg gtgcaaaaga ctctagataa cttttgatac taaaataaag
     3061 ctccttgtca ttgacaacga ttttattaaa agaatctgta agtaggtcgc ggataaggct
     3121 agatgattta tccagctcgc taagcaattt aagaggcggt actgcattgt acaattggct
     3181 gtgaatatgc tcccatttac ttacaatagc cttcatttct tcatggaggt cagctacttt
     3241 tttgccctca gcagctgtac gtatgattac tccaaaattc ttgggtttga tagattctac
     3301 cagtacctgc agacgttttc tctcttccgg gttatctatt ttttttgata ttgaaataat
     3361 gtcagaaaag ggtgataaaa ccatatacct gcccggtagt gcaatctcac aacttagtct
     3421 tggtccttta gtagagattg gttcctttag catttgtacc agcactgcct ttctcttact
     3481 aaacacattt tcaatcttat catttttaat gatttcaggt tcgatcgtaa aatggtctaa
     3541 acgcgaggta ttctggttgc catttagaga attttcggtg tatgccaaca aagaattgag
     3601 tctgggtcca agatcagtat aatggagaaa ggcatccttt tcgtatccga tatctacaaa
     3661 tgcagcattc agtgaaggat tcagtttttt gattcttcct aaaaaaatat cgccaacagc
     3721 aaagttactg ttggtctttt ggtgatgtag ttcgactaac tttgaatcct caagtaaggc
     3781 tatttcgacc tcatttggag aaacatttat gataagttct ttttccacaa tggtaaaaac
     3841 taagattaac aaaaaacacc aatagggaag ccaatgtgct tatgctacat agcagaaatg
     3901 cacaaagtac tttacccgaa ctacaaacct acttgcaaaa ctcaggtaaa atactatgag
     3961 caaaatctaa cattaggtgg actgcatcag gctacaatcc ctgaagtgta aaacagatta
     4021 atactatctg ttcttcttct tatgtctgtt tttacgaagt ctttttttac gcttgtgtgt
     4081 agcgatttta tgtctttttc tttttttacc gcaaggcata atttatcgtt taatattttt
     4141 aaaattcatt ttttacattt tttctctgcc aatccggcat ttgttttgtc accaaggcca
     4201 gtataagcat ctgcaagcaa acaatagata ctgacagtac ccggcgacaa ttctacagcc
     4261 tttttcaacc tctccacagc tttctcaaac tgtcctgttt tgatggctag ccttccgagc
     4321 tgtatattta catctgcatt ttccggatgt ttggtattca gctcacgcaa cataagaata
     4381 cccttcatcg gattgtcttc cagcggctta tcaacatata ccattgccag ctgcatttga
     4441 taattgacat tcgtagaatc gagacgaatg gctttttcaa aagctctgat tgcccgacgg
     4501 ctacaaacat ctcttacttt ttcatccaga ccaccctccg aaagtgcgat gcggtaggta
     4561 gtaccggtta tctggtaagc ttccttatct ttcagtatat cagaaaccat atcggcataa
     4621 tatcctgaaa cctcaggctt accggattga taccagaatg acgccagttg tttgagtgat
     4681 tctacctttg ttgaatcatt ttttgtagag gataatacac tttctaaagt atgcagtgta
     4741 tttttttgct ccggtgtcag tttggctgtt gcttcttcct ttgacgccag tagcactgtt
     4801 tcataggttt tatcctcctt tgtctttgta gcagcaggag gttccgcttt cggaacttct
     4861 ccaaaaccaa aatacagcac gaatgtcagt aagactgcgg aagaaacggc tattatttgg
     4921 tttttgctca acaaaattgt ataatattaa tcttcttctt caatatcttc aggcatagac
     4981 gtcacattgt cttttacctg gtcaacaaac atttttgcag ccttgaaaga cggtatgaaa
     5041 tgttcaggta tctctatggc aaaattttgc ttaatatgtc ttccaatttt tttagctctt
     5101 ctcttgataa caaaacttcc aaaccctctg atataaacat tttcaccttt ggcaagggta
     5161 gccttcacct ctttaaaaaa agtctcaagt gataccagta cgtccacctt tggaacgccg
     5221 gtcttatctg atatgatact tacaagatct gcttttctca tgtggatata cttaattggc
     5281 tattagatag ttatacaatt tattctcttc ctttttcgtt tcgaaccgca aagatgagaa
     5341 tttttctgat taaatgaaag tccttaaccg atattcttag gtgtaagatg aaaattattt
     5401 tagatatgct tatttgcagg tttatatttg gctaagccgt acttttgctg ttcaattctg
     5461 taatatgctc cattcgatga ctggctttgg aagtgccagg gctacatacg gcccaaaaaa
     5521 tataactgtt gaaatccgtt cggtcaacag caaatttacc gatgtcaagt ttaaactacc
     5581 tcaaaactac cgtgataaag aatttgatat taaaaaaata ctcacagatg atgtcacaag
     5641 aggtaaggta gaatttaatc ttagcattca aagcaatgac ggccctgcac aagtaagtat
     5701 caataaaaat ttatttagaa aatactacaa cgaactcaca ttgctggctc aagaacttaa
     5761 tatgccaaca ggtgatatta tgcaggcaat acttcgcatt cctgaagtaa tcaatgctga
     5821 cgaacaaacc atacccgatg aagaatggga gctagtacgt aaaaccatat cggaagcagt
     5881 ggctacattt caaaattaca ggctggccga aggtgaatcc ataaaaaaag atctgatagc
     5941 aagaatagac agcattcagc aacttcacaa caagattgac cctttcgcta caagccgtat
     6001 ccaaaacttg cgcatccggc taaaacaact actcgacgaa cattcgcttt cagacatggt
     6061 tgacggtcat cgcttcgaac aggaaatact cttctacatg gaaaaaatag acatcaatga
     6121 agaaaaagta cgtttgacac aacattgcaa gtatttttta caagaactcg acagtgacga
     6181 catccaggta ggaagaaaac tcggcttcat cagtcaggaa ataggacgcg aaataaacac
     6241 actcggagca aaagcctatg atgccaacat acaacgcctg gtagtaatga tgaaagatga
     6301 gctggaaaaa attaaagaac atgtagctaa tattgtgtga tgcatgaata aaatgattat
     6361 cattaccgca ccatccggtg ccggcaaaac taccatcgtt aagcatctgt tgaaggtatt
     6421 cccgacattg tctttctctg tctctgccac tacgagagca ccacgcccac acgaatcaaa
     6481 tggtaaggaa tactatttca tcagcacaga tgaatttcaa caaaaaatta gaaaccacga
     6541 attcatagaa tacgaacaag tatatgaaaa ccaatactat ggtacactac acgctgaagt
     6601 tcaaagactg tggagcgagg gtcgctcaat catttttgac atagatgtaa aaggtgccct
     6661 caaccttaaa aaaatattca tgaatgaagc attctctatt tttttaaaac caccttcatt
     6721 tgaaatttta aaagaaagat tactcaaccg taagacagaa gacaaagcct ccttaaacaa
     6781 aagaataaag aaagcaaagc tcgaactaaa atatcaggat aaatttgatt acgttctcat
     6841 taacgataaa cttgaaattt ctttagccga agctgaaaat ttggtacgca cattcttaga
     6901 aatacaataa gttttcttaa ctttccaact ccaatcaaac atttctacta tgggcagcat
     6961 ccagtctttg gtcacaaaag gtattaaaat aagtgtcgaa tcatttttcc aaagtgaata
     7021 ttcgaatgct attgaaaaca aatacatttt tacttacaaa gtaaaaatcg aaaacaataa
     7081 ttcatttgca gtaaaactac tgcgcagaca ctggtatatt tttgattcaa atggattgtt
     7141 ccgcgaagta gaaggcgaag gcgtcatcgg acaacagcca atgatagaat caggtgacga
     7201 acatacgtat gtttcgtggt cacaactcga gacagatatg ggtcaaatga atggcaaata
     7261 tactatgctc aacttaaatg ataattctgc ctttgaagta gaaatacctt tctttcaact
     7321 tgtaatgcct agtaagctca actaacttca agcttatttt gcttaaaaaa actgtacaaa
     7381 aatcgtaaaa gtccagattt ttgtgcagtt tagcatattt caaatattct ttttccctac
     7441 ttcaatgaga atctcactgt aataccggct gatgtattgg tcgtagggaa agattctgtc
     7501 gtctttggca atattcttga atactcccag aacaaacgaa gattaagcct cttgttgagc
     7561 tggtaatcgg cattaggggt aattctgata gtagtagaac cacgtgttgg gttgctgata
     7621 tcctggtcaa gtaagtggtt ggtagtaaga tcatcgcggt acgagaagtc aactttgatg
     7681 ttcaggtcac gtccggtgtt ggtagttccc tttttcgaag atggcaactt cttgtcatcc
     7741 gtttttttct tgacaggtgc ttttttgacc aaaaaaggaa tgtcgatgtt cttgattctg
     7801 taaccaaagc caaaaacata ttctgttgta tttgtctcaa acaacgtatt gtcaacgaaa
     7861 gacatctgca gatttctcga tttcttcata tcaactctgg cactcatatc attttttaac
     7921 tttacatcaa cacctaacag cgggctcagc tgctcggtaa tgacaatagc cggaatctcg
     7981 tatctcgaaa aatagttatt ggtaataggc tgcagattct ctaacggatc attcggatta
     8041 tagaatgcat tcgtgttgaa attactgatg gtaagtgttg acgtataacc atgtgtaata
     8101 ttgacagagc taaaaatatc tttaaggagt ggaagtttat ttaagcctcc gtacgacaat
     8161 ttccagttag gtaatggaag cttactgaat aatttttcat tcagcactac tttggatgga
     8221 ttatcacctg agtaagctgc aataaatgcc ggtacaagaa cttcctgctg ataacgacca
     8281 tatccttctg tataattgcc atacagataa tcagttacgt gctttccggt gcccaatcgt
     8341 tgtgaaataa tctccctatt gtcttcaaat ttcttaaaca agccattaac atttttgtca
     8401 aacaaggtat tgatggccaa atatgagatg gtgtacgatc caacctccct cactgaaaga
     8461 tgctgaaaat cttcggttac ggatggtttc ttgaagtctt cttgtctgct gagtgcataa
     8521 ttttgcttca aatctacatc tagcttgaag tcattgaatg gttcgagttt tatttgcccc
     8581 gatatgtctc ttgtataatt gcgtgatacg ggttgattga ggaatacact ctctgtaaac
     8641 catcctttat cagccgactc atcgaaccac ctgtccgtag gtctttcacc taagataaaa
     8701 ggtaaaccag gagctgtgaa cccttgattc atacccagta gattggtctt tggtgtaaag
     8761 cccggcacca catttgtata gctttcagaa taggtaagcc tacctttgcg taccatcatt
     8821 agcggtctga taacaatttt ttctatgata gaaggctctc tgtctttctt atccttttta
     8881 tcagtcttag tatccttcaa atccggatct ttggcatttt tcttttcttc aggcttaccc
     8941 ggatctttca ttacacctct tgcatctaca gaacgttttg gtgcttcctt cttgttaatt
     9001 tttttcagat atttgatctt gttgtacaat gcttcaaaat ccagatcagc gtttagttgc
     9061 ctctgttgtg tattttgaat cacattgccc agcgaatcgg tattgagcga tgcggtgttc
     9121 caactataac ttgagttgac attagccctg atggttgtcc agtctgtaat aagcaaaccc
     9181 ttggtgggca gagtatagtt gacaccgata ttgtgagaat acgttttggt acgtccaaat
     9241 ttcttgaggt tatccttaat atatttgctc ttctcagcat ctgtatagaa aacattcgtc
     9301 tccggattga tagtattctc cggtctttca tctattaatg actgattatt ggcactgaat
     9361 gttaacttca gtgacctggt aaggtcccaa ttaaggctgt aattacgatc ccacgaccac
     9421 cttttgtcgt agaatgtatt cttggtaggg tctgtacctg caaagcggta tgaggtagcc
     9481 tgcaaagttc tgtccatagc attgcgcagt acaaatccgt taggaatagg gttaaaattg
     9541 aagtcagaca acaatttaag ccaagcggca tcctttaata cttttttcag cggctcaacg
     9601 tattttacct gtcttgtaaa attataatcg agtgctcctt tataattctt aatccgatcg
     9661 gacgaaataa tcgggtcatg atgatccgtc tgcgtgtagg catagcttgc agcgaaattc
     9721 tctacatccc acggtttggg ttttacagcg gtacctgtcc tttcttttct tacattggta
     9781 aagttaaaac ccttaatgtt tgtataatct tcggcactgc tcctcaccga gtcacgtaca
     9841 tttttgtcat caatattatc gagtttttcc tttagtggaa tgtcaagatc gtatgggtca
     9901 tactccggcg tacgggtcgt attagagact tgagcgagga acggtatctt caaccctgta
     9961 ttagggggta gaaatttact tagttcgagc tctacagccc cgtcatactg tactacttgc
    10021 tcacgttgtc gttcagcaag tctttgctcg agattgcccc aacctatcga agtaaaatta
    10081 cctgcaacat tcacccttcc caggtctgcc agctttactt cagcacgggc aagggctgca
    10141 tagccactgt tttcgtcaaa tccgttgact cgtagctcat tgacccacag ctctgcacaa
    10201 tgtggtattt tatcattatt ttttacacct accataaagc ccttcaccaa accgaggttc
    10261 gggttaccga taacacgtac tttattcgaa ggaaaatcag ggtctgccat ttcaaaaaca
    10321 tcgataagcg aaatgcccga tttgtttctt tcctgctttg cttccttgaa taatttaagc
    10381 aaaatgtcaa gattattagc tttgcgccat acttctgctt cgtattccgg tgtgccgggc
    10441 gtataagtat tatccggatc tgataattct ataggtattt cgtattcgta gtaattattc
    10501 tggaagtcgc ttcccattct taagaaaact gataaaccgc ctggggcaat tttttcctgc
    10561 gactcagcat gtacaaacat tttcatacgt tcgaacacgc gaaggtccat accagacaat
    10621 ttgtaaatgg cacgagaatc tccgggtttc agattgcaga catttatagc aatcgactgt
    10681 tcattctgca acaaactctg agcattactt agagtatttt cgcgtataat acccggtggt
    10741 gtcacatagt taaatggcaa tttctgactg ttctcttcaa tgcttaccga ctttacatcg
    10801 aagcttgtct tgttatcagg atctacttca atgactgatg agaacggacg cttatacctt
    10861 ctccattgac ttcttatcaa ctccagtgta gcaaagcgca aagttacttc ttgattaaat
    10921 ccggtaaggt acattcttat aaaacgaata cttctgaagt cctgaatacc acctactttt
    10981 ctcgaatatt cttgaatagg gaccacaaaa cgataccatg tacggttaga acctgcaggg
    11041 ccatcaattt tttcaacgac atacgggttg ttttcaatac ctacattgcc atcgggcaca
    11101 agaggaattt tgtattcgaa atatgattct gcctcattca gcgtattatc caggatcaca
    11161 tcctcagagt caggattctg tgtagaacta ttgataagac catccttagc aggctggttg
    11221 tttccttcag taccattata tcttgaatat ctgtcataca cagtagagct gctgttccaa
    11281 acaggatcct ggaaagaaat aaaattgtca tttgaagggt ctttcattgc ctcattcata
    11341 gcttccggtg tcagatatcc ctgatactgg ttaagaatat ctgcaaattt taccctttca
    11401 tcatcatcat tagcaccatc aagacctata tcttgctgct ttctcacctc agggtcagca
    11461 tcgaacgaat tattgatagg tggtattctg ggtacatttg accaaacagt tctatcgact
    11521 ttcagattcg gattcttgac gtttggcagg gcattttcag aaaacaacct tgaatctctc
    11581 agaatatctt ctgagacgtt accaatatta atgtacagat ctccaggatt agcagagttg
    11641 tcaataaaag gacttaaaag ccaaaactca atatactcgt agttagcagc ttcgaaatcg
    11701 ttgttgttct gaagatttcg cataataccg ccccagcgag tcgccggttc ttttaagctt
    11761 gcgtttttct ctattccctt tgaataggta gtgcccagag gcaagtcgaa gttatacggg
    11821 ccttttactg taggctcata atgcaggtcg aatgtctgaa tattggtatt gtatccagtt
    11881 ggtatctgtc tgttggggaa aagttccttg attggtatgg cagttacgta attattgtct
    11941 gcacctgccg aagttgccct tacggtttcg tcaattctgt accatgccag tttggcacgg
    12001 ttgacacctg acctgagatc atcaataagg ctggattccg ggaacaattc ttttttatca
    12061 ttgcctgcca tctgaggtac agaggccagc acccaattgg tagcaggttg gcgtatatca
    12121 aatccgtttg tactaccttc gaagtcatct acatacacgt ttccgccttt ttcggcactc
    12181 tcattaactg cgctggcatg gccgggtttt aatgcagcag cttccgctga aaaattaata
    12241 gcggacattt cttttgtctg tattagtggt atcttgtcta ttgcctttgt cagccatggc
    12301 agatccttct taagtgttat gtcggcaccg tagatcttgt tattaatcgg atcctcacca
    12361 atgttcactt tttgagtaaa tggtctttca aacaagttga ggaatgtacc acctacatta
    12421 atatccttac cgagcttgta atctgctcta agccctagca tggtgcgtgt ctgaaaacta
    12481 aaaagagaat tgtcttcaaa tgaaatattg ataggtacgc ccgattgcaa gtaagagtca
    12541 ttcaggatct tgacacggcc tgtgttataa tctaccgaat aatcaacatt tggcgtaagt
    12601 gttacaccac ctgcagtgac agttacgcta ccttgtggca ggttaaacac accaagtgaa
    12661 atgtctgatg tagtaccggc cttgaaggac cctttaagag taaatctgtt gagctcagga
    12721 aattctcttg cctgtgtaac agttgaatca tacagctgat agtatccgta ttttttacga
    12781 agattggcat tgtctatctt atcacccaaa taactgccaa atggctccag tacagggaac
    12841 atgattcgac cagacctcgt attgacagtc agcccaggca caaagtcaaa gattccgtcc
    12901 gggaatgggt cattccgttt attgagctta tcgagattaa acaactgtat gagtggctct
    12961 ccggcaagat tactcgatgg taggaatctc ttttctccac ctcccggatc ttcataataa
    13021 atgtcgagtt tgaagtcttc ttgctctaca ttgtaagcac caatagaata aaagtttttg
    13081 gccatcaggt cccacattgg caggtcaacc ctagatgttg tgcttttgag catttttacg
    13141 aacaacacgc ccaatgtatc cgggttattc ggattttcgg tactaagctg acctacccgg
    13201 aatgtcttac cgttgtaagt atattcgtaa gccacaccaa gcacatccga aggcttcatg
    13261 ttaatattca tcgagatgat accaagctcg ttattaatgg tatattcatt atcattgagg
    13321 agtctggccc gtaccttttc gaagtcgcgt atttggctaa atttgtattc cggccctgtt
    13381 agtgtcgcta ctgctttgtt gatggttttt acatcaggcc ttgttataag gctttcgtaa
    13441 atatcgttcg aactattggc cggaagctcc ttgccgtaga tatccggatt cctaattccg
    13501 gcaggttttt gaaactgagg gttagaattg gtaattctct cgctctcacc aaggtcggta
    13561 atggctacga tgtctctgac cttttcagta acattacggt cattggtgac ccacacttca
    13621 attttattta atctgaagag cgaattaatc tgtggaaggt tttcgagtga acgttcgaag
    13681 ttattcctgt tgtaatgtga cagaaagaag tgcctgttct cgtcatactc atcagctttt
    13741 acagcgaatg tctgcagttc cttaccacct tttagttcta ttgccttacg tcttgatttt
    13801 tgctgtgagg ccaccgcagt aagtcgaaga ttcccgaatt gtagttctgt tttgacaccg
    13861 aatagcgagt tattaccttt tatcaatgaa cttttcaacg gtagagaaac attacctgct
    13921 tctatagacc gaatgatttc atcttcatta aattttgtac tgttgtagtc tatcttcatc
    13981 tgattttcga aatcaaatgt tgcacggttg ttgaactgtg tagagagatt gagcttctca
    14041 ccaatcttac ccgtcacgtt catctgtata ttcatatcaa aatcgaatcc gccggttgtc
    14101 tggtttcgga tcgggtaagt agggttttct acttttgagt aatcatagcc aaatgtcagg
    14161 tcaatattac cggttgggtt aatgacaggc tctgtccctc caaacaagcg attgagcaaa
    14221 cttttattgg gaatgtatcg tgcgagagga ttcttagggt catacttttt gcctgaactt
    14281 tttgaaacgc ctgacagata atcaaaatat tcgcgttcct gttgctttga tttataatta
    14341 agatattcat caaatgacat tttgaccggg gccttgtaat attcgttacc tattttttca
    14401 gacaatatgt attgtccgct tgcggggtca tattcctcag atttttcgac tgatgccgga
    14461 tctttcaggt ctattagtgt tttgggcttg taaagaggat attcagtctt tttttcagga
    14521 atggtatccg ttacttcact acttgttgat cctgccggat gatgatatgc tacacgtggt
    14581 atctgtggca gattcagcat tgcaaacgag aaaacgccta caagaaccgt caacaaaaaa
    14641 gaaataatca gctgtttata attcatatta ttattcaggt ctgcaattcg attattaacg
    14701 tctatccgtc attttaatca tatcaaggcg caaatttaac aaatataagc tttgtttaat
    14761 atgttaggtt aatgaacttg atacgattat tactgaaaat agtttttgat tttaagattt
    14821 aagatagtct gccaagtgct ttttttataa gaacttctac tgaacttata ccaggctctt
    14881 caagcaaaat tttgttcaac acctgactgg cagccggcct cataaatcct aaagcaagca
    14941 atgcaaataa cgcctcatct tttattgtat tgtccgagac aaccaaattg atatttacat
    15001 tagaagaatc tttcgaaatt ttatctttta ggtcaagaat aatacgtttg gcagtcttcg
    15061 gcccgatacc ttttaccctg ctgaatgttt tgtcatcttc ttgtatgatg gctgctttta
    15121 tttggtcggg tgtgagggcc gacagaacaa gttgcgctgt ggatgcacca ataccactaa
    15181 cggagatgag ctgtaaaaaa agtatttttt ctgattcctc aaaaaaacca taaaggctgt
    15241 gactgtcttc ctttatcatc aggtgagtag caagctttac ttctgacaat tgctcaagct
    15301 tagaaaaggt atgcagactg atgtttatgt ggtatcctat gccctgtatg tctattatta
    15361 taaatgtagg tgatttatag gcaatcgggc cttttatata tgcaatcata cttacaattt
    15421 taattttgaa caaatgttaa ttgcggcaaa tgtacgattt ttgacataaa gacaggaaag
    15481 agaacgataa ttgcatttac cagataatct ccattccgtc atatccgagt tttacatttg
    15541 caggcagctc tttattcaaa tttgcctctg taccaaggtg gtggcttatg tgtgtgaggt
    15601 aagccatctc aggattaatt tgttctatca attgcaatgc ttcactaaga ctcagatgcg
    15661 aatggtgttc ctctcgccga agagcattga taaccagata acgggttcct tttatcaggt
    15721 caatttgatc cggctcaatg aattttacat ctgtcatata ggtaaaatcg cccactctga
    15781 acgccataac aggcatctga ccatggctcg cctctatggg caatatattg attccgccta
    15841 tcgtaacgga tttgtttttt tcgagtggtt ctaccttagc gcgcggagct ccgggatagg
    15901 gttcttcttc aaatgcaaag ctgaatcttc ggtaaagttc tgcctttacc ctatcagttg
    15961 cccagaaagg cataatcatg ttttgaagaa aattaaaagg tcttatttca tccagcccga
    16021 ttatatgatc gttgtgttcg tgcgtaagca ggatagcatc aaggtgtgca atacctattc
    16081 tcagcatctg ctgcctcata tctggcccgc agtctataag tacattggtt tttccgtctg
    16141 ttataagtgc tgatgtacga aacctactgt catgcggatt gggcgacatg caaacctcac
    16201 atctgcagcc gatgacaggc acaccctgcg aagtaccggt acccaataga gtcgctttca
    16261 agcttcagta tttgaagatt cccatgaatg aataaggtgt ttgagtaaat ctttgtcctt
    16321 ggtatcaagt ttttcttcat caatgtctat ctgcagaatg atctctatca gattgtttat
    16381 cctaccttct gtgtcaaaaa atttattgat gaccaccacc ttatttgact cgaccaaaca
    16441 ataccccgac tgaaagttac ccttttcgta cctgacgtca tattcttgtt ttccaagaaa
    16501 atcttccagt ttattcagaa aatgtttggt gtattttatc atacagtgta atattgagcg
    16561 tttattgtgc aaatataatc aattaatagc atgcttttat caaaaatata cctaacactc
    16621 tgtacaacct tgttattcat aataagccac aatctgcatg cccagacatt tggtgcaact
    16681 atccaggcgg gtctcgttgc ttcgcagata gacggtgacc aatacgcagg ttataacaaa
    16741 cccgggcctt ttattggcat caatggtatt gcgcgtctta aagaaaagtt ttatatagaa
    16801 accggtattg catacgtatt cagaggtagc caatccgaat ttttgcagga caattccatt
    16861 ccacaattta aaataaacct ccagtatatt gatatacctg taacattcca ttacaaagac
    16921 tggcttgtac ctgaagatga attctacagg atggactttt atgcaggtgg ttattttggc
    16981 agactaatcc acagctatgc tgaatacagc ggatatgacg atgtcctcga tttgttcaat
    17041 aaaaatgagc tcggtattgt cattggcgga gcatacagtt ttggaccaca cgggtcatta
    17101 ggtgctaaat ggtgcagagg tcttaactac ctttttaaac ataatacgaa tggagtgaat
    17161 caaaattccc ttattaaccg tttcatacag ttttattatc aatacaaatt ctaattatga
    17221 gaaatgtttt cttcgtaacc atgctctgtc tggctgttct tgtggcttgc aaaaatgaaa
    17281 ctgctaccca gaccacactt acaacagctc agcctgagaa tgctcctaca tcaaccggca
    17341 taactgcagg accagccgct acattgccca tcattcctct tgaagaatac aaggcccttg
    17401 ccgaaaatac caactcaatt gatattgttc cttttgaatc aaatctctct atgagtattc
    17461 ctgacaaggc ttcttgctat cagtttgttg ctaaccatat tctcgacaca caagtatcag
    17521 aaatgccatg tacaaaaccg aatggcagaa tattcttcaa taaagatggt gattctatcc
    17581 tcgaagctaa tatttattat accgagcagt gcaaatatct tgttttctat aaagcaggta
    17641 acaacacacc actatacgcc acacaaataa ccgagcaagg taaaaaatat ttcgagtcaa
    17701 tatatggtgc caatgccatg caggagctcc agaaactaca aagcggcaat caaaaatagt
    17761 tgcccgaaaa acttcctttg tttataggtg agttctaata ttttacggta gtattttcac
    17821 taccgcgcac tttccttttg gcacatttag ccatttgttt gaattaaaaa ctcattagaa
    17881 ttttcaaact tctggcattc aagatgcggt tgttgcaact tgcagcttga aaatattgta
    17941 tagaaatatt taaaatataa aaatatttct ggttttaaaa atgaaatgaa attttaacaa
    18001 aatgaacaaa caactaaaaa ccaaaggaca ccaatttttc ctgtttcttg ccctattgct
    18061 atcattatta gcttgtaaaa ataacacaca ggaaaactct gcagagtcca atactgtgaa
    18121 tggtgaaacc ctcggaacca tacttgacaa acttacagaa caatactaca atcctgacaa
    18181 tacttatgct gcaggtgcca gactggcgca ttacgactca ctgctcaaag ttacccaaga
    18241 cattcagagt tcgcttaatt acgcagtatt acatgccaat accttgctcg agtatggaaa
    18301 ggaaaaacgt gcagtagcag aacttgaaaa aatttatgac tacgtcaaat cggttcctgc
    18361 atcaagactg gcagccggcg aaatgctggg tgtagcctat atgcgcctgg cagaacgaac
    18421 caactgtata gccagacata acgcagatgc atgcatcttg ccaataaaag ggctcggcat
    18481 ccaccaggat aaaggtcctg caagaaaggc tgtagagatt tttgaaaaaa tactgaagga
    18541 agatcccaaa atgctcaact caagatggtt acttaatatt gcttacatga ctcttggaga
    18601 atatccgaat ggtgtaccga aggaattcct cattcctgga atagggcagg gtgaaacaac
    18661 taccgtaaaa ccattcaccg aaatggcttc agatcttggc atcgccttat acaaacaaag
    18721 cggaggaacc atagttgatg attttgataa tgacggcaat cttgacatcg tagtcaccgg
    18781 ctggagtctt aaagaaacac tccactatta tcacaacaac ggcgacgggt cattttctga
    18841 tttttccgaa cgttcgggcc ttaataaaat acgaggtggg ctcaacatga tgcaaacaga
    18901 ttataacaac gatggcaaaa ttgatatttt cctacttcgt ggagcctggc agggtagtgg
    18961 gccttttgcc aaagtaccaa attcacttct taaaaataat ggtgacggca cattcacaga
    19021 tgttactatt caggcaggaa tattgagctt caacccaact caggctgcca cctggaatga
    19081 ttttaataat gatggctggc ttgatgtatt tatcggaaat gaatccttac cttcaaacaa
    19141 tatttatcca tcagaatttt atataaacaa ccaggatggt acttttacca acatcgcatc
    19201 accacaaggc ttaaatatta aaacctttgt aaaagcttgc acatcgggcg attatgataa
    19261 tgatggctgg gcagatattt ttatttcatg ccttacaggt gaaaaaattc tgctccgcaa
    19321 caaaggggta aaaggcaagg taccaacatt cgaaaatact agtcagcagg cgggtctggc
    19381 tcaaaacaag gagcggacct tccctacatt tttctttgat tatgataatg acggttggct
    19441 tgatcttttt atatgcaact atagcttcga gcagcagttg tctttcttcc tcgctaagga
    19501 aacactatac ccttcaaacg atcaggaagg aaaaattata ctttatcata acaaccagaa
    19561 tggtaccttt agtgaagtat cggggcagat gcacctcaat caacctgtat ttgccatggc
    19621 tgccaatttt ggcgattttg acaatgacgg ctggcttgat atgtttatgt caaccggaaa
    19681 cccaaactac cgttcgctca ttcctaaccg cctctacaaa aacatgggtg gcactgactt
    19741 tatggaggtt acttactctg cccgtgtagg aaatctgcaa aaaggccatg gcgtagcatt
    19801 tgctgatatt aacaataacg gcgaccagga catatacgtt aacataggtg gtgcctacga
    19861 aggtgatgct tttaattctg cattctacct taatcctggc cagaatgaca ataactggat
    19921 aaccatgcag ctcgaaggag taaagtcaaa caaagcagct atcggtgcaa ggattcggtt
    19981 aaaatttact gataatggca aacaaagatt tgtgtacaga gacctgaact ccggtggtag
    20041 ttttggtagc tcgcctttga gaagagaaat aggcttagga caggcaaaaa tgattgatga
    20101 aatacaaatc aagtggcctg caagtggtac cgtacagaca atcactaaca tcaaaccaaa
    20161 tcaattcatc aaaataacag aaggaaaaga aggttactca accatcaacc tcaagaatat
    20221 agtctttaaa tacaagtctg gtggcatccc tatgtgtgca ccgcgtgttt caatataggg
    20281 ttagatgtgt aaggtatttt tatgaagatc gcctattcgt cgtcatcatc cggcggacgg
    20341 ttatgtccaa gttggtcttc tgcctcgagt ttgtcgaaca gattattcag cctgtacatt
    20401 tcatccagag tatcatctag atagaagtat atttcaggta ttcttcttac atgctttttg
    20461 atcctgtggg caagggcttg tcttattctt actagctgat cctccaacat cagaacgaca
    20521 gcttgctttt cttctgtatt atagacactc acatacactt ttgcctgact catgtcgggc
    20581 gacaccatca cattggtaac tgtaacgagt gcatcaccat aaatataact accttccgaa
    20641 ctgagtacaa tacttacatt ccgtctgatg atttcgccaa tttgtttttg tcttatt
//
LOCUS       DAOQQY010000021        18405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_924_length_18405_cov_7.300109, whole genome shotgun sequence.
ACCESSION   DAOQQY010000021 DAOQQY010000000
VERSION     DAOQQY010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18405
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_924_length_18405_cov_7.300109"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLE23_02290"
     CDS             <1..523
                     /locus_tag="PLE23_02290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82124.1"
                     /translation="ENVKTIKSDGGNRKWFGTDNGVFVQSADAKTLVYKFDTKNSPLP
                     SNSITSMAINPDNGECWIGTSKGLAVYRMESTVGGPTHKSEVYAFPNPVRPDYAGNII
                     ITGLAVDSDVKITDTNGKLVYQTNSIGGQAVWDGNDYNGRRANTGVYLVFATGTSDPE
                     NPNGLVTKILFFN"
     gene            complement(537..1670)
                     /gene="bshA"
                     /locus_tag="PLE23_02295"
     CDS             complement(537..1670)
                     /gene="bshA"
                     /locus_tag="PLE23_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HMX82125.1"
                     /translation="MKIGILCYPTYGGSGVVATELGKGLAKAGHKIHFISYRQPARLT
                     HYSENIFYHEVSNVDYPLFEYPPYDTALTSKVVDVVKYEDLDLLHVHYAIPHAAIAFM
                     AKQILLTYGKHIPIITTLHGTDITLVGNNRTFSPVVEFSINHSDGVTAVSNSLAQDTY
                     SNFNIEKDIKVIYNFIDFERFKKLDKDHFRKAIAPLGEKILIHTSNFRKVKRVDEVIR
                     IFAAVHKVIPSKLLMIGDGPERQNLEDLCRDLSLVDHVRFLGKQDAIEEILAIGDLFL
                     IPSASESFGLSALEAMACEVPVISSNIGGLPEVNLDGITGYLCDVGDHQTMAQRAIEL
                     LGDEQKLETFRKNALQQARRFDIEHILPEYENYYQQILNNFKQ"
     gene            1745..3172
                     /locus_tag="PLE23_02300"
     CDS             1745..3172
                     /locus_tag="PLE23_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82126.1"
                     /translation="MDISIKNSLLLICLYLAAIPVYAQKFYLSEPFRSKMNDDIELVG
                     QQNGFVFFTQESDHDFRILRLNAELYNNRTLILPLNERKTKVRYTAIVDKMLTVVYSY
                     RVKSELCIKIVRYDTDLNLRDSLTMACFGDAGYSPDIKAIPSKDKKTLVLYYIQAANY
                     IQCYSFNLHTQKLNWAYRWDHTLEKTEYRYFEKILVDNGGNAVLIFNEPGTGKHDHNI
                     SCGYLTATGVVTRQFKNQPYNLYEADWLIDDKNQRLILAGMYSKDKSYRAEGVLYWRV
                     RLPLDNDTPLLSTNPFNTARITGFESERNNFENGIDGISVVEILLKHDGGVVLILEKR
                     QQNERNTRGRLIESGNTIRNSTDYYYDDVYICSVNANGTTDWLAPLAKKQFSQDDGAD
                     FSSFLFMKGKDYLRLVFNDEILNDNTISEYLIRPDGTFARKNIPNKDLRKLKIMFRKG
                     KQVSVGSGIVPCYVKNKLRYLKIEY"
     gene            complement(3181..3876)
                     /locus_tag="PLE23_02305"
     CDS             complement(3181..3876)
                     /locus_tag="PLE23_02305"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HMX82127.1"
                     /translation="MFTDYTYTIIDLFPVSAGDVLVNLLVAFFCGLILSLIYRLTYRG
                     PSYSVSFVNAIVMLTIISALIIVVIGNNLARAFGLVGAMSIIRFRTAVRDTMDMVFIF
                     LSLGIGMAAGVGLSVVALTGSLISGMIIMALTYTNYGQARRRFHVLQLAYDDSVFDQK
                     KIDNLFTQYARTNKLVSMKYSGNDTNMEAIYHIALKRDKYADALIAGIRKEKGISYVN
                     VFYDEDDINPPTV"
     gene            complement(3902..4681)
                     /locus_tag="PLE23_02310"
     CDS             complement(3902..4681)
                     /locus_tag="PLE23_02310"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HMX82128.1"
                     /translation="MMRSEFKYKVPLERLSELRAAILPFVAPDKFASKQPGNQYTVRS
                     IYFDTRNFDMYHTKTDHLAHRLKVRLRGYNIGDEYSNVTCEIKRKYEGPILKNRASLQ
                     YNMVQAIYNGADLDTILAVTDEQIGLRKFFYQIFSGSLQPVVTVIYEREAYESVILDK
                     ENGIRLSIDKNLRSVPYPAIEELFVERDIMYAYQGYFILEVKFNKYCPAWVKPILAEF
                     GLLKGPASKYVMCIDSQPVINTARRWDSLIRSGALTKKQKT"
     gene            complement(4693..5760)
                     /locus_tag="PLE23_02315"
     CDS             complement(4693..5760)
                     /locus_tag="PLE23_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82129.1"
                     /translation="MNRAIALLLIFCFLLALLFLPVKIPYTLRSIGNVLPSQEWRLVQ
                     DGTGSLSASLQDNQSGAITRVSSWQFERGDLFDLQMAIDPNSGKMFQKGDTIVRIYSS
                     ISNQQILDLENLIHVKSAIGKELSTGEKKTIVQEAEERLASAKEFYELKSRELAVAQK
                     LIADGVIADIEYQRIKNEHDVAKIEIQVAEKTLQVASTGAKSETVEVNTTEVNTLKKQ
                     LAFLKGRNLKYVITAPFDGVVGRLYELGQLIVLHRMSECVVQVPMKVEEMAYIGRNLR
                     ILITDPSSMETYNAKLLSVASQTQVVNMRSVGFLICMINTQPGKPFPTLGNVGNCEVH
                     CDDLTPLQYIKRLMKFRIGAG"
     gene            complement(5931..7241)
                     /locus_tag="PLE23_02320"
     CDS             complement(5931..7241)
                     /locus_tag="PLE23_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82130.1"
                     /translation="MTTDDTRQRNILFLAYFFPPIQTIGVWRNYFLAREAKKSFAKVF
                     VSGRRIEESSRDDKVNYETFHYLSNPAPDYRFLLGGKKKPGFSEGSKKNVISKFLIRL
                     VNSFPFNIFLGEGGIIYIVSSVWKAHRLIREENISHIYSSFRPMSDHIVAWCLKSLHR
                     ELIWTADFRDLPFDPLYRRYFWFGFQRWVLSGILRKADIVSTFHEGIGYGLRQFSDKE
                     ITILENGTFQDFEREKNAEQNPKFTIQYTGSLFQDERNPAILMQALGELLEEKIISQE
                     EIRLNYAGKDSAKWASLARQHRLSDVSVNHGLVSREQASVLQASAHLNLLLTSSHPEY
                     QGILTGKFFEYIAAGRPILCIINGCRDQSVERLFDKYHLGMVVYLDEPNPGVLKDFLM
                     KLLDDFKQNGKLNWQVDEKIYEEFNWQRTMEKWLEKLSLVKFNA"
     gene            complement(7238..8425)
                     /locus_tag="PLE23_02325"
     CDS             complement(7238..8425)
                     /locus_tag="PLE23_02325"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMX82131.1"
                     /translation="MKKHKNSNGAIAEKIRNPLINFSGKRVAIVSNTCWNVYNFRINV
                     IERLLALKAKIFIIAPIDETIQHLSRLEDVTIIPLNHLKRNSSNPISELILLRELVTK
                     YRSVKPDFIIHYTIKPNIYGSVAAGMLGIKSVSVVTGLGYTFINQPWMKWLVSKMYRF
                     AFRYNERVIFENQDDRNLFVQSGISSDKNSVSIKGCGVNVDYFKTKISRLDRENLEFI
                     FVGRLLFDKGIVEYVEAAKYIVQKYPHVRFTVLGLLDEENPSHISRETLLTWVKSNAI
                     RYYGETKDVRPFLKRADCIVLPSYREGLPRVIIESMAMALPVITTDTAGCRETVAEGV
                     NGFLVPIKNTDALAVAIEKFIKMTQGARFMMGQRSRQMVEAEFRDTVIADSILTVVAQ
                     SLV"
     gene            complement(8430..9509)
                     /locus_tag="PLE23_02330"
     CDS             complement(8430..9509)
                     /locus_tag="PLE23_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HMX82132.1"
                     /translation="MAVKPYIVIMAGGIGSRFWPASRESMPKQFLDILGLGRSLIQLT
                     YDRFASWIPAQQIMVLTHADYKNLVIKQLPDLPVHNIICEPARRNTAPCIAYGAFRIS
                     KKDPDAVMVVVPSDHLILKEEAFRIALNMATNFAAANDALLTLSIKPTRPDTGYGYIQ
                     FAEPNDAGVCPVLNFTEKPPIEKAKAFLAEGNYAWNAGIFIWSVSSVLKAFMHFAPSI
                     YKLFERALPHYFTESEESYMQHHYQQADNISVDYAIMESADNVFTIPADIGWSDLGTW
                     TSLFEESSHDKNQNACNTTLLLAKDSSENMIRGQNGKLMVIGGLENFIIIDEKDVLLI
                     WPKDDEQSIKEVTKQAAEKFRGIFN"
     gene            complement(9511..10440)
                     /locus_tag="PLE23_02335"
     CDS             complement(9511..10440)
                     /locus_tag="PLE23_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HMX82133.1"
                     /translation="MMQKNSRIYVAGHRGMVGSAIVRLLEAKGFTNILTSTSTETDLR
                     NQAQVNAFFEKNKPEYVFMAAAKVGGIHSNNTYRAEFLYDNLMIEANIIEASRKNEVE
                     KLLFLGSSCIYPKMAPQPIREEHLLTGTLEYTNEPYAIAKIAGIKLCEAYRDQYGCNF
                     ISVMPTNLYGPNDNYDLNNSHVLPAMIRKFHEAKKNGSASVVIWGTGTPRREFMHVDD
                     LADACFFLMENYNEKEFVNIGFGEDITILDLAKLVKDTVDYEGEIIHDLTKPDGTPRK
                     LMDSSKLNGLGWKPATTLAEGIRAVYEEVKHKL"
     gene            complement(10437..11549)
                     /gene="gmd"
                     /locus_tag="PLE23_02340"
     CDS             complement(10437..11549)
                     /gene="gmd"
                     /locus_tag="PLE23_02340"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMX82134.1"
                     /translation="MKKALITGVTGQDGAYLADFLLQKGYEVHGVKRRSSLFNTGRID
                     HIYEDPHIENQHFKLHYGDLTDSTNLIRIVQEVQPDEIYNLGAQSHVMVSFETPEYTA
                     NADAVGALRILEAIRLLGLAEKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYGVAK
                     LYAYWITVNYRESYGMYACNGILFNHESPIRGETFVTRKITRGVARIALGMLDKIYLG
                     NLDAKRDWGHAADYVKAMWLMLQQDKPEDFVIATGITTTVRDFVKKAFAELGVELSFN
                     GKDENEKAVVVSCSDPEYFIEPGTVVVEIDPRYFRPAEVDILVGDATKANTKLDWYPE
                     HSLDDLVKEMVQSDVELFRKDKLLMDAGFKVKNEFE"
     gene            complement(11795..13087)
                     /locus_tag="PLE23_02345"
     CDS             complement(11795..13087)
                     /locus_tag="PLE23_02345"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HMX82135.1"
                     /translation="MNKIIFRLLVLQVTFLLYQTAQVYAQDFAYKDSVYVDYIKSVKF
                     NIAGNPLALPAVPLGSEFPLELSFDDMDTHTREYTVSIIHCNKDWTPSQEINELEYLS
                     GFNNAPIRDISISFNTVVNYMHYYMSLPNSEIGWKITGNYLLKVTDRDDDDKVLITRR
                     FVVFQPLMTIVPAVTRTAEVSKSTTHQELDFEVQHKGFVIRSPRTELTATILQNKRWD
                     TAIKDLIPVFIRSEGEVFDYQDKIVFEAGKEFRFFDMRGMHILGENVENARQVANGYE
                     VHLRKDRINTNVSYLYRKDLNGDYVVDCYDVDVNDCQTKGDYGNVYFTLKSDTELEGK
                     DVYITGAISDWQIKPPFKMEYNESRGQYEAQILLKQGYYNYQYTTVDRKTGRQDDNEL
                     QGNWYETENSFTIILYYRGFGERYDQVLGYGVFDSRKY"
     gene            complement(13191..14672)
                     /gene="guaB"
                     /locus_tag="PLE23_02350"
     CDS             complement(13191..14672)
                     /gene="guaB"
                     /locus_tag="PLE23_02350"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMX82136.1"
                     /translation="MEKEKNTRPKFISDALTYDDVLLVPAYSEVLPREVDISTRLTRD
                     IRLNVPIVSAAMDTVTDYRLAIAIARQGGIGIIHKNMSIEDQAEQVRIVKRSESGMII
                     DPVTLTPDALVADALDLMRKFSIGGIPIIDIRGKLTGILTNRDLRFEKNHSKLVKELM
                     TTENLITAPEGTDMAKAKEILQRNKIEKLPVVDKNNKLVGLITYKDIMKVQNYPMSCK
                     DSLGRLVVGAAIGVTGDMFDRIEAVVKMGVDVVCIDTAHGHSKGVLEAVKETKKKFKD
                     LQVIGGNVATGEGALALVNAGADGVKVGVGPGSICTTRIVAGVGVPQLSAIYNAATAV
                     AKKGVPVIGDGGIRYTGDIAKAIAAGADTIMAGSLFAGVEEAPGETVIFEGRKFKVYR
                     GMGSMGAMELGSKDRYFQDAEDEVKKLVPEGIEGRVPYKGTLEEVMTQYIGGLRAGMG
                     YCGASNIPNLQKAQFVRISMAGIKESHPHNITITKESPNYSAR"
     gene            14719..15195
                     /locus_tag="PLE23_02355"
     CDS             14719..15195
                     /locus_tag="PLE23_02355"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMX82137.1"
                     /translation="MPEKISFSIYNDEYFMRQALKEAEKAYELDEIPIGVVVVCNNQI
                     IARAHNQTEQLNDVTAHAEIMAITAASDYLGSKFLDECEIFSTLEPCNMCAGALYWAR
                     PKRIIYGAGDDKRGFMLIGKSMLHPKTKLEYGILNEECSTLVKKFFNKKRKPKPGE"
     gene            complement(15256..15837)
                     /locus_tag="PLE23_02360"
     CDS             complement(15256..15837)
                     /locus_tag="PLE23_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82138.1"
                     /translation="MKNILLFFCFFAAMSLSAQDNKYTGHLGGTLGFSTAQSNAKNSV
                     VKKVWSFSPEIGIYPFAHWGAGINFLIEGVSQGNYITTSYGCGIYVRRYFPVTDNFSM
                     ILGLNGSYKTTKAVQKFPQGNIEETENGIGAFFDLGMSYQLSPRWSLIGRIGTLGYNK
                     TTNPDNPDYGEKVFGINLNALGNPFSVGALYKF"
     gene            complement(15917..16543)
                     /locus_tag="PLE23_02365"
     CDS             complement(15917..16543)
                     /locus_tag="PLE23_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82139.1"
                     /translation="MMKIHKLFILYIGILLLSSCNKEDTFISEDLRQYYKYKDRIGGT
                     ALKSVPRAGSVEWRFDANLWHSKYGDGYLLSLSTSSPYYAFVEDADVAREVLAFDFEV
                     DKVLIKDYSAYLSSDWKVGSPFVIVQYHRYLADGDVSGGTWNIDPTKDNKFKITGIDQ
                     GGKTCTGEFELYFIRNDDFWDFNYRKDTRQFARRINFLNGKFTAKIAY"
     gene            complement(16540..17712)
                     /locus_tag="PLE23_02370"
     CDS             complement(16540..17712)
                     /locus_tag="PLE23_02370"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HMX82140.1"
                     /translation="MFNSEVGGKRIRVKTIDFEPDFVYHPKKYIEDDLDALTISTNAK
                     YLVNYYKCDIGVLLTDRGTYTSKKDTSAVIYGAANSLDPDALNKFCIVETPFIDPIRY
                     TFAHEVAHQFGSLHENDFENSTGCPHGKVLDFGNYYRYTIMARAPHYGRIPYFSNPDV
                     SYMGVSTGETDYRDNAHQIDSRAFCKVEDNKNLTIFEAKIETGVVCAELPLTLDADVE
                     HGFFAPPAVLSVAPYGVGPYQYEWYWSLNGLFNPSNYLGNTSSIYLPQPPNCPAFYVS
                     LKVTSADGYVSFAKKRIVCDQNCPRSSNLISDVSNMGLVYPNPSSGLVNIAEIDGEAI
                     VRAELFDLKGNLIKSIDYNSIERNAAVDFRGVSQGLYFLRLYSIRNSVIQKVIITQ"
     gene            complement(17749..>18405)
                     /locus_tag="PLE23_02375"
     CDS             complement(17749..>18405)
                     /locus_tag="PLE23_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82141.1"
                     /translation="TSVHQYISTSVHQYISTSVHKKFLILALVIMSLMKLEAQTYNYV
                     FQYTSPPPTLGIDQTKIFNYLSSLPYNDTLYYLQLSPNIEDQGYVYMNLPDGNVSFKI
                     QDILFDGDTSFTLIASAPQGNLSLYKTSTAIGGTIDLGNTVYALYPFGTSLHVLMKYD
                     LTKMTPGDCTVADSAFAAISGEIAMCGTTCDKTYVDVLAMVTPDARLWLDNHWGIYAN
                     "
BASE COUNT         5274 a         4053 c         3499 g         5579 t
ORIGIN      
        1 cgaaaacgtt aaaaccataa agtctgacgg tggcaaccga aaatggttcg gtaccgataa
       61 cggtgtcttt gttcagtcgg cagacgccaa gacacttgtt tataaatttg acacaaaaaa
      121 cagcccgctg ccaagtaatt ctataacttc aatggccatt aaccctgata atggcgaatg
      181 ctggataggt acaagcaaag gtctggcagt gtatcgtatg gaatcaactg taggtggccc
      241 gacacacaaa tcggaagtat atgcattccc taacccggta agaccggact atgcgggcaa
      301 tattatcatc accgggttgg cagttgactc ggatgtcaaa ataaccgata ctaatggaaa
      361 actggtctat caaacaaact caataggcgg tcaggcagtg tgggatggga atgattacaa
      421 cggacgacgt gccaatacag gtgtttatct tgtatttgcg acaggaacat ctgacccgga
      481 gaatccgaat ggtctggtta ctaaaatact tttttttaat taagcagcaa accgaattat
      541 tgcttgaaat tatttaatat ttgctggtag taattctcat actccggcag aatatgttcg
      601 atgtcaaatc ttcttgcctg ttgtagcgca ttttttcgga atgtttcaag cttttgttca
      661 tcaccgagca attctatagc gcgttgtgcc atggtttggt gatcacccac atcacaaaga
      721 taacctgtaa taccatcaag attcacctcc ggcaaaccac cgatgttaga agatataacc
      781 ggcacctcac acgccattgc ttcaagcgcc gacaatccaa aactttcact ggctgaagga
      841 atgaggaaca aatctccaat cgccagaatt tcttcaattg catcctgctt gcccaagaaa
      901 cgcacatgat caactaagct gaggtcgcga caaaggtctt cgaggttttg acgctcaggt
      961 ccatcaccta tcatcagcag tttggatggt atcactttat gtactgctgc aaaaatccgg
     1021 ataacttcat ctactcgttt tactttcctg aagttggagg tgtgtatcag tattttttca
     1081 cccaacggtg caatggcttt tcgaaaatgg tctttatcga gtttcttaaa ccgttcgaag
     1141 tctatgaagt tgtatattac tttgatgtct ttttcaatat tgaaattgct gtatgtgtct
     1201 tgtgccagac tattggatac agccgttact ccatctgaat gattaattga aaattcaacc
     1261 accggcgaaa atgtacggtt gttccctacc agcgtaatat ctgtaccgtg cagtgtagtt
     1321 ataataggaa tgtgttttcc atatgtaagt agtatttgct tggccataaa agcaatggct
     1381 gcatgcggta tggcataatg tacatgtagc aggtcaagat cttcatattt tacgacatca
     1441 accaccttgc tcgtaagtgc cgtatcataa ggcggatatt cgaacaaagg ataatcgaca
     1501 tttgatactt catgataaaa aatattctca gaataatgtg taagacgagc cggctgtctg
     1561 taagaaatga agtgaatctt atgacctgcc tttgcaagac ctttcccgag ttcagtagca
     1621 acaacaccac tgccaccata cgtcggatag caaagaatac ctattttcac gaaactgttg
     1681 taattttaat ttaaaaatac gaatacttaa ataataactg aaataaatat ttgttcaatt
     1741 agttatggat ataagtataa aaaatagttt actgctgata tgcctctatc tggctgccat
     1801 accggtatat gcccaaaaat tctatctgag tgaaccattc agatcaaaaa tgaacgatga
     1861 tattgagttg gtaggccagc agaacggatt cgtgtttttt acacaggagt ctgaccatga
     1921 ttttagaatc ttacgactca atgcagagct ctataacaac cgtacactca ttttaccgct
     1981 taatgaaaga aaaaccaaag tccgttacac tgctattgtg gataaaatgc ttactgtagt
     2041 gtacagttac cgtgtcaaat cggaattgtg tataaaaatt gtacgttatg acacagatct
     2101 caatctgcgt gacagcctca cgatggcttg ttttggtgat gcagggtaca gccccgatat
     2161 taaggcaata ccaagcaagg acaagaaaac tctagtacta tactacatcc aggcggccaa
     2221 ctacatccag tgctattcat tcaacctgca tacacaaaaa ctcaactggg cttaccgttg
     2281 ggaccacact ttagaaaaga ccgaatacag gtattttgaa aaaatactag ttgacaatgg
     2341 tggcaatgct gtcctgatat tcaacgaacc cggcaccggc aaacacgatc acaacataag
     2401 ctgcggctat ctgaccgcta ccggagtagt tacccgacag ttcaaaaatc agccctacaa
     2461 tctctacgag gcagactggc ttatagatga taaaaaccag cgtttgatac tggcaggaat
     2521 gtactcaaaa gataagagtt atcgggcaga aggtgtactc tactggagag tgcgcctgcc
     2581 actagacaat gatacgcctt tattaagcac gaatccgttc aacacggcca ggataaccgg
     2641 atttgaaagc gaaagaaata attttgaaaa cggtattgac ggtataagtg tagtagaaat
     2701 tctgctgaaa catgatggtg gagtggtgct catacttgaa aaacgtcagc aaaatgaacg
     2761 gaacacaaga ggcagattaa tagaatctgg taacactatc cggaactcta ccgattatta
     2821 ttacgatgat gtgtatatat gttcagtcaa tgctaatggc acaacagact ggctcgcacc
     2881 tctggccaag aaacaatttt cgcaagatga tggtgccgat ttttcttctt ttctattcat
     2941 gaaaggtaaa gattatcttc gtttggtatt caatgacgaa atactcaacg acaacaccat
     3001 cagtgaatac ctaattcgtc cggatggaac gtttgcacga aaaaacatac caaataaaga
     3061 tttgcgcaag cttaagatta tgttcagaaa aggcaaacaa gtaagtgttg gttcgggcat
     3121 tgtgccatgc tatgtaaaaa acaaacttag gtatttaaag atagaatact gaatatcgtt
     3181 ctacacagtt ggcggattga tgtcatcttc atcataaaaa acattgacat aagaaatgcc
     3241 tttctctttt cgtatgccgg ctatcagagc atccgcatat ttgtctctct taagtgctat
     3301 atggtaaatg gcctccatat tggtgtcatt gcccgaatat ttcatgctca ccagcttatt
     3361 ggtacgtgca tattgagtaa aaagattatc aatcttcttc tggtcaaata ctgaatcgtc
     3421 ataggcaagc tgcaatacgt gaaatcttct ccttgcctga ccgtaattgg tataagtaag
     3481 tgccattatt atcattccgg aaatgagact gcctgtaagt gccacaacac tcaacccaac
     3541 acctgccgcc ataccaatgc ccaaacttag gaatataaac accatgtcca tcgtatcacg
     3601 tactgctgtc ctgaaccgga tgatgctcat ggcgcctacc agaccgaagg cacgtgccag
     3661 attattgccg ataaccacta tgattaatgc cgaaatgatg gtaagcataa caatggcatt
     3721 tacaaatgaa acagaataag acggccctct gtatgtaagc ctgtaaataa gtgacaggat
     3781 aaggccacaa aagaaggcta cgagtagatt gacaagcaca tcaccagccg aaaccggaaa
     3841 aaggtcaata atagtgtaag tataatctgt aaacatcagt agcttttcat tttctgaaca
     3901 attaagtctt ttgtttcttt gtcaatgctc cgctccttat aagcgaatcc caccttcgtg
     3961 cagtattgat taccggctgt gaatcaatac acatcacata ttttgaagcc ggacctttca
     4021 ataacccgaa ttctgccaat attggtttta cccatgccgg gcaatattta ttaaatttca
     4081 cttcaagaat aaaatatccc tggtatgcat acattatatc tcgctctaca aatagttcct
     4141 caatagcggg gtatggtaca gaccggaggt tcttgtcaat ggataatctg ataccgtttt
     4201 ctttgtctag tataacgctt tcataggcct ctcgttcata aataactgta actaccggct
     4261 gaagcgatcc ggaaaatatc tggtaaaaaa atttccttag cccaatctgt tcatcggtca
     4321 ctgccagaat agtatcgagg tctgcgccat tgtaaatggc ctgtaccata ttgtactgca
     4381 gacttgccct gttctttagt atcggacctt cgtatttgcg tttaatttca caagtaacat
     4441 tggagtattc atcgccaatg ttatatcctc gtaaccgtac tttgagcctg tgagccaggt
     4501 ggtcggtttt ggtgtgatac atgtcaaagt ttcgggtatc gaagtaaata ctccttacgg
     4561 tatattggtt gcccggctgt tttgaagcaa atttatcagg agctacaaaa ggaagtatgg
     4621 ctgccctcag ttcagaaagc cgttctaacg gaactttgta tttaaactct gacctcatca
     4681 tagcatttta ctttaacctg ctcctatcct gaatttcatc aggcgtttta tatattgtag
     4741 tggtgtaagg tcgtcacagt gtacttcaca gttacccaca ttgccaaggg taggaaaggg
     4801 ttttccgggc tgagtattta tcatacaaat caaaaatcct acactcctca tattcactac
     4861 ctgggtctgt gatgcaaccg acaacaattt tgcattgtag gtttccatac ttgaagggtc
     4921 ggttatcaaa attcgcagat tgcgaccaat ataggccatt tcttctacct tcataggcac
     4981 ttgcaccaca cattccgaca tacgatgaag cactataagc tgaccaagct catacaggcg
     5041 gcctaccacg ccatcgaacg gagctgttat cacatatttc aggtttctcc ctttcagaaa
     5101 agcaagttgt tttttcaggg tgttgacctc ggtagtattg acttcaacgg tttctgactt
     5161 agcaccagtg cttgctacct gtaatgtttt ctccgctact tgtatttcta tttttgcaac
     5221 atcatgttca ttcttaatcc tttgatattc gatatcggct atcactccgt ctgctattag
     5281 tttttgagcc acagccagtt ctctgctttt aagctcataa aattctttgg cagaggcaag
     5341 gcgttcctct gcttcctgta caatagtttt cttttcaccc gtagaaagtt ctttaccaat
     5401 ggccgatttg acatgaatga gattctccaa atctagtatc tgttggttgc ttatggaaga
     5461 atatatccgt acaatggtat cacctttttg aaacattttt ccagagttcg ggtcaatagc
     5521 catctgtaag tcaaataaat cacctcgttc gaactgccag gaggataccc ttgtaatggc
     5581 accactctga ttgtcctgaa gcgatgcaga cagacttccg gtaccgtctt gtaccagacg
     5641 ccactcttgt gaaggtagca cattgccaat actccgcagc gtatatggta tctttaccgg
     5701 cagaaataat aaggccagta aaaagcaaaa tatcagcaaa agagcaattg cacggttcat
     5761 ttaaaatttg accaggttag ttttacctct cagtgatgtg taatatatat tattctcaca
     5821 gtttatgaca agattatcaa gctgtatatt atattatcca ttattcaaac acacaaagta
     5881 aaccattttt tatttctttt tgagcgggtt ttgtctattg attaagaata ttaggcatta
     5941 aacttaacta aggacagctt ttcgagccat ttttccattg ttcgttgcca gttgaactct
     6001 tcatatattt tttcgtccac ctgccagtta agttttccgt tttgcttgaa atcgtctaat
     6061 aatttcatca aaaaatcctt caatactcca ggattaggct catcaaggta aaccaccatg
     6121 cccagatgat acttgtcaaa taagcgttct acgctctggt ctcggcaacc attgatgata
     6181 cacaatattg gtcgccctgc tgctatatat tcaaaaaatt taccggtgag tattccctga
     6241 tattcaggat gtgaagatgt aagcagtaga ttgaggtgag ccgaagcttg caaaacggat
     6301 gcctgctcac gacttacgag tccatgattg acagatacat ctgacaatct gtgttgccgt
     6361 gcaagggaag cccattttgc tgaatcttta ccagcataat tgagccttat ttcctcttga
     6421 gaaatgattt tttcctccaa caattcaccc aaagcctgca ttagaatagc cggattgcgc
     6481 tcatcctgaa acaatgatcc agtatattgt atagtaaact ttggattttg ctcagcattt
     6541 ttttctcttt cgaaatcctg aaatgtacca ttttccagta ttgttatttc tttatctgaa
     6601 aattgccgga ggccataacc aatgccttca tgaaatgtgg aaacaatgtc cgcttttctt
     6661 aaaatacctg acaaaaccca gcgttgaaat ccaaaccaaa aatatcgtct gtacaacgga
     6721 tcgaatggca gatcgcgaaa atcagccgtc catatcagct ctctgtgcaa agatttaagg
     6781 caccatgcca caatatggtc agacattggc ctgaaagaac tgtaaatatg gctgatattt
     6841 tcttccctta tcaagcggtg tgccttccaa acagaactga caatatagat gataccacct
     6901 tcgcccaaga aaatattaaa cggaaagctg ttgacgagtc ttataaggaa tttcgaaatg
     6961 acattcttct tggaaccttc actgaagcct ggctttttct tgccaccgag tagaaaacgg
     7021 taatcgggtg caggattgct caggtagtga aatgtctcat aattaacttt gtcatctctg
     7081 ctactttcct cgatacgcct gccagacaca aagacttttg caaaagactt cttcgcctca
     7141 cgtgccaaaa aatagtttct ccacacaccg atggtctgta tgggtggaaa aaaatatgct
     7201 aaaaacagaa tatttctttg tcgagtatca tctgttgtca tactaaactt tgggcaacaa
     7261 cggtgagaat gctgtcagct ataaccgtat cgcggaattc tgcctcaacc atttgccgac
     7321 tgcgttggcc catcataaat cgggcacctt gtgtcatctt tatgaatttt tcaattgcta
     7381 cggcaagcgc atcggtattt ttgatcggca ccagaaaccc atttacacct tcagcaaccg
     7441 tctcccgaca gcctgccgta tctgttgtaa tcacgggcaa tgccatagcc attgattcga
     7501 tgataaccct tggcagacct tcgcggtacg aaggcaggac tatacaatca gcacgcttca
     7561 gaaacggtct cacatctttg gtctcgccat aataccggat ggcattgctt tttacccagg
     7621 tgagcagggt ttcacgacta atatgtgacg gattttcctc atcgagcaac ccaagtacag
     7681 taaatcttac gtgaggatat ttttgtacaa tgtattttgc tgcttctacg tattctacta
     7741 tacctttgtc aaatagcaac cttcctacga aaatgaactc tagattctct ctgtcaagcc
     7801 gactgatttt ggttttgaaa taatcgacat tgacaccaca gccttttatg cttacagaat
     7861 ttttgtctga agaaatacca ctctgaacaa aaagattgcg gtcatcctga ttttcgaaaa
     7921 ttacacgctc attgtacctg aaagcaaagc ggtacatttt actcaccagc catttcatcc
     7981 aaggctggtt gatgaatgta tagccaagcc cagtgacaac actcacgctt ttgatgccaa
     8041 gcatgccggc tgccacacta ccataaatat tgggcttgat agtataatga atgataaaat
     8101 ctggttttac cgacctatat ttcgttacta attctcttaa aagtataagt tctgaaatcg
     8161 ggtttgacga attgcgtttg agatgattga gcggaataat ggtaacatcc tctaagcggc
     8221 tcaagtgctg aatcgtttca tcaatgggtg caatgataaa tatttttgcc ttaagagcta
     8281 aaaggcgttc tatgacgtta atgcgaaaat tatatacatt ccagcatgta ttggaaacaa
     8341 tggcaacgcg tttgccggaa aagtttataa gcggattccg gattttttct gcaattgcac
     8401 cgttactgtt tttgtgcttt ttcatgcttt cagttaaata ttcccctaaa cttctcagca
     8461 gcctgtttcg tcacttcctt tatgctttgc tcatcatcct taggccatat aagtaataca
     8521 tctttttcat caataataat gaaattttcc aaaccaccta ttaccattag ttttccattt
     8581 tgtccgcgta tcatattttc tgacgaatct tttgccaaaa gtaatgttgt gttgcaggca
     8641 ttttggtttt tgtcatggct cgattcttca aataaggatg tccaggtacc taggtctgac
     8701 caaccgatat ctgccggaat ggtaaaaacg ttatcagctg attccattat cgcatagtca
     8761 accgagatat tgtctgcctg ctgataatga tgctgcatgt agctttcttc gctttcagta
     8821 aaataatggg gcaaggcgcg ttcgaatagc ttgtaaatag aaggtgcgaa gtgcataaat
     8881 gccttcaaca ccgacgatac cgaccagatg aagatgcctg cattccacgc atagttgcct
     8941 tctgccagaa atgcttttgc tttttcaatg ggtggttttt ctgtaaaatt aagtaccggg
     9001 catactccgg catcatttgg ttctgcaaac tggatataac cgtagccggt atcgggccgt
     9061 gttggcttaa tacttaaagt taatagggca tcattggcag ctgcaaagtt agtggccata
     9121 ttaagtgcaa tcctgaaagc ttcctccttg agtataagat ggtcggaagg cactaccacc
     9181 attactgcat ccgggtcttt tttgctgatt ctgaaggcgc cataagcaat acacggagcg
     9241 gtattccgtc ttgccggctc acaaatgatg ttgtgtaccg gtaagtcggg cagttgcttt
     9301 atgacgagat tcttgtagtc ggcatgtgta agtaccataa tctgttgggc tggtatccag
     9361 gatgcaaacc ggtcgtaggt gagctgtatc aatgaccttc caagtcccaa gatatcgaga
     9421 aattgtttgg gcattgattc tctgcttgcc ggccaaaacc tgctaccaat gcctcctgcc
     9481 atgatgacga tatatggttt tactgccata ttacaatttg tgttttactt cttcatacac
     9541 tgcacggata ccttctgcca gagttgtagc cggtttccat ccaagaccat tcagttttga
     9601 actgtccatc agttttcgcg gtgttccgtc tggttttgtc agatcatgta ttatttcgcc
     9661 ttcatagtca acagtatctt ttacaagctt tgccaggtca aggatggtta tgtcttcacc
     9721 aaaaccgata ttgacaaact ctttttcgtt ataattttcc atcaggaaaa aacatgcgtc
     9781 agccaaatca tccacgtgca taaattcgcg tctgggtgtt cctgttcccc aaatgactac
     9841 tgatgctgag ccatttttct ttgcttcatg aaatttccgg atcatggcag gtaatacatg
     9901 cgaattatta aggtcgtaat tatcattggg cccgtaaaga ttcgtcggca tcacagagat
     9961 gaaattgcag ccatactgat cgcggtaggc ttcacataat ttaataccgg ctatttttgc
    10021 aatagcataa ggttcgttgg tatattcgag cgtacccgtc agcaggtgtt cttccctgat
    10081 aggttgtggt gccattttcg gataaataca tgacgaacca aggaacagga gtttttcaac
    10141 ttcattcttc cttgatgctt ctatgatatt agcttcgatc atgagattat catagagaaa
    10201 ttcggcgcgg taagtattgt ttgaatgtat tcctcctacc ttggcagcgg ccataaatac
    10261 atattccggt ttgttttttt cgaaaaatgc gttgacctgt gcctggtttc taaggtcggt
    10321 ttctgttgat gtgctggtaa ggatgttagt aaaacccttt gcctccagca atcggacaat
    10381 ggccgaaccc accattcctc tatgacctgc tacgtatatc ctgctgtttt tttgcatcat
    10441 tcgaattcgt ttttcacctt gaagccggca tccataagca acttatcctt cctgaataat
    10501 tctacatccg actgtaccat ttctttaaca agatcgtcaa gcgaatgttc tggataccag
    10561 tcgagcttag tgtttgcctt ggtagcatca cccaccaaaa tatctacttc agcaggacga
    10621 aaatatctgg gatctatttc tactactacc gtgcccggtt caataaaata ctcagggtct
    10681 gaacatgata ctactactgc cttttcattc tcatctttac cgttaaaact gagttctaca
    10741 ccgagttcag caaaagcttt ctttacaaaa tcgcgaaccg ttgtagtaat gccggtagca
    10801 atgacgaaat cttcaggttt atcttgttgt agcatgagcc acatagcttt cacataatcg
    10861 gctgcatggc cccagtcgcg tttagcatca aggttgccaa ggtagatttt atccaacatc
    10921 ccgagtgcga ttctggctac tcctcttgtt atttttctgg ttacgaaagt ctcgcctctt
    10981 atcgggcttt cgtggttgaa gagaattcca ttgcaggcgt acattccgta tgactcgcgg
    11041 taattgaccg ttatccagta agcatacagc ttggcaacac catagggcga ccgcggatag
    11101 aatggagtgg tttctttttg tggtatttct tgtacaagcc cgtaaagttc tgatgtcgat
    11161 gcctggtaaa atctggtttt ttctgccaag ccgagcaatc gtattgcttc aagaatacgc
    11221 agcgctccta cagcatcagc attggctgta tattcgggcg tctcgaagct caccattacg
    11281 tgcgactgtg ctccgaggtt gtaaatttcg tccggctgca cttcctggac gattcgaata
    11341 aggttggtag aatcagtaag atcgccataa tgtagtttaa aatgctggtt ttcgatgtgc
    11401 ggatcttcgt aaatatggtc aattcttcct gtattgaaca atgagctcct tctcttaacg
    11461 ccgtggactt catatccttt ttgtaataaa aaatcggcca ggtatgctcc atcctgaccg
    11521 gtaacaccgg ttataagtgc ttttttcata attttaataa attaaatatt tcgactgcac
    11581 aggcaaagtt actaagtaaa tatgatactt tttagaacaa atgaaatgca agactgatat
    11641 tcggcaaaat gaaggatact tcttttggaa aaaatttgtt catataccta cttagccaaa
    11701 agctatctcc atttcttttg ccggacagga aataagtgtt gaataggaat caaacagcta
    11761 ctgaaatgca gttttacagt tggacataat actatcaata cttcctcgaa tcgaacactc
    11821 cataaccaag cacctggtcg tagcgttctc caaatcctcg atagtaaagt atgattgtaa
    11881 atgaattttc tgtctcatac cagttgccct gaagctcatt atcatcttgt ctgccggttt
    11941 ttctgtcaac ggtggtatat tgatagttat aataaccctg ctttaacaat atttgggctt
    12001 cgtactgacc tcggctttca ttgtattcca tcttgaacgg tggttttatc tgccagtcgg
    12061 atatggctcc ggttatatac acatccttgc cttcaagttc ggtatcactt ttgagtgtaa
    12121 aatacacatt tccgtaatct cccttagtct ggcagtcatt gacatctaca tcgtagcaat
    12181 ccacgacata atcgccgttg agatcttttc tgtagagata agagacattg gtgtttatac
    12241 ggtcctttcg cagatgtact tcgtagccgt ttgctacctg cctggcattt tctacatttt
    12301 cacccaatat gtgcatacct cgcatatcga aaaaccggaa ttccttaccg gcttcgaaca
    12361 caattttatc ttgataatca aatacctcac cttccgaacg gataaaaacg ggtataaggt
    12421 cctttatggc agtgtcccat cgtttgtttt gcaatatggt agcagtcaac tccgtcctgg
    12481 gcgaacgtat gacgaagcct ttgtgctgta cttcaaaatc caactcttga tgtgtggttg
    12541 actttgacac ctccgctgta cgggtgactg ccggtacgat ggtcatcaat ggttgaaaaa
    12601 ccacaaaacg ccttgttatc aatactttat catcatcatc tctgtctgtt acttttaaca
    12661 gataatttcc cgtaattttc caccctatct cactatttgg caaactcata tagtagtgca
    12721 tgtaattcac cacagtatta aaggatattg aaatgtctct tataggtgca ttattgaagc
    12781 cgctcaggta ctcgagttca tttatctcct gtgatggtgt ccaatcttta ttgcaatgta
    12841 tgatagacac tgtgtattct ctggtatgcg tatccatatc gtcaaatgac agttcgagcg
    12901 gaaactcact gcccagcggt acagcaggca atgcaagcgg atttccggca atattaaatt
    12961 ttacgctctt gatataatcc acatatacag aatccttgta ggcaaaatcc tgagcatata
    13021 cttgtgcagt ttggtaaagt aagaaggtga cttgcaaaac caagagtctg aaaatgatct
    13081 tattcattat tatttgattg ctggcaataa cttacaacac acggcacaac atatttgtta
    13141 tagggttgac aataaaacgg tagctcaagt gctatattgt acccggcatc ttacctggcc
    13201 gaatagtttg gcgactcttt ggtaatggta atattatgcg gatgactttc cttgatgccc
    13261 gccatagata ttcgcacgaa ctgagccttc tgcagattgg gaatattgct tgcaccacaa
    13321 tatcccatac ctgccctgag gcctccaata tattgagtca tcacttcttc aagtgtgcct
    13381 ttgtagggta cccgcccttc gataccttcc ggaaccaatt tttttacctc atcttctgcg
    13441 tcttggaagt agcggtcctt agaaccaagc tccatggccc ccattgagcc cataccacgg
    13501 taaaccttga acttcctgcc ttcgaatatc accgtttcgc ccggagcttc ttctacacct
    13561 gcaaacaacg aacctgccat aattgtgtca gcacctgcag ctatggcttt ggctatatcg
    13621 cctgtataac ggataccacc atcgcctatc actggaacac cctttttggc cactgctgtg
    13681 gctgcattgt agatagctga taattgtggt acacctactc cggctacgat gcgagtagtg
    13741 cagatacttc ccggacctac acccaccttc acgccatcgg ctcctgcatt gacgagagca
    13801 agagctcctt ctccggttgc cacattgcca cctattactt gcaaatcctt aaattttttc
    13861 ttggtttcct taacagcttc aagcacgcct ttcgaatgtc cgtgtgctgt gtctatacac
    13921 actacgtcaa ctcccatttt tacgactgct tctatcctgt cgaacatatc accggtaacg
    13981 ccaatggctg cacctaccac cagtcttccc aggctatcct tacagctcat cggataattc
    14041 tgcaccttca taatatcctt ataggtgata aggccgacca gtttgttgtt cttatccact
    14101 acaggcagtt tttcgatttt gttgcgctgc aatatttcct tggccttcgc catgtccgtt
    14161 ccttcaggtg ctgttattag gttttcagtc gtcatcaatt ccttgaccag tttggagtga
    14221 ttcttctcga accgaaggtc tctgttggtc aatatgcctg taagcttgcc ccttatatca
    14281 atgataggta tgccaccaat gctgaacttt cgcatcaggt caagtgcatc tgctaccagt
    14341 gcatcgggtg tgagcgtcac agggtcgatg atcataccac tctcagagcg tttgacgatg
    14401 cgcacctgct ctgcctggtc ttctatcgac atatttttgt gaatgatacc gataccacct
    14461 tgccttgcga tagctattgc cagcctgtaa tccgttacag tatccatggc tgctgacaca
    14521 atgggaacat tgagacgaat atctctcgtc agcctggtcg aaatatcaac ttcacgaggt
    14581 aaaacttctg aatatgccgg cactaacagt acatcatcgt aggttaatgc atcagaaata
    14641 aatttggggc gagtattttt ttctttttcc atgtgcaaag atataatttt gacctgaata
    14701 ttcaaaatca tttcagcagt gccggaaaaa atcagctttt caatctacaa cgacgaatac
    14761 ttcatgcgtc aggctctcaa ggaagccgaa aaagcctacg aactggacga gataccaatt
    14821 ggcgtagtcg tggtgtgcaa caatcagatc attgcacggg cccacaacca gacagaacag
    14881 ctcaatgatg tcactgcgca tgctgaaatc atggccataa cggctgcatc cgattatctt
    14941 ggatcaaagt ttctggacga atgtgaaatt ttctccacgc tcgagccttg caacatgtgc
    15001 gcaggtgcgc tctactgggc acgacccaaa cgcatcatct acggcgcagg tgacgacaag
    15061 agaggcttta tgctcatcgg gaaaagcatg ctgcacccaa agacaaaact cgaatacggc
    15121 atactgaatg aagaatgcag cacattggtc aaaaaatttt tcaacaaaaa aagaaagcca
    15181 aaaccgggag aatgatgggt tactccttcc cagctttggc tctctgtttc tgcattattt
    15241 cacactgcgt ctaacctaaa acttatacag cgcacctacc gaaaacggat tccccagagc
    15301 attcagattg attccaaata ctttctctcc ataatcagga ttgtccggat tggtggtctt
    15361 gttgtaacca agcgtgccga tccggcctat gagactccat cttggtgata attggtatga
    15421 catcccaaga tcaaaaaatg caccgatgcc attttcggtt tcttctatgt tcccttgtgg
    15481 aaacttttgt accgctttag tcgtcttata actaccattc aggcctaata tcatactgaa
    15541 attatccgtt acaggaaaat aacgtctaac ataaatacca caaccataac tggtagtgat
    15601 ataatttcct tgcgacacac cttctattag aaagttgatt ccagctcccc aatgagcaaa
    15661 aggataaata cctatttcag gactaaatga ccaaaccttt ttaaccacag aattttttgc
    15721 attagactga gctgtgctaa agcccaacgt accaccaaga tgccctgtat atttattatc
    15781 ctgagcacta agggacatag ccgcaaaaaa gcaaaaaaat aacaatatgt ttttcatgtt
    15841 ttatatttaa tcaggtaata cctatgaagc taaagatagc atcgtaccat accggatgtg
    15901 acaataattt taattttcaa taagcaattt tggcggtaaa cttaccgttt aggaagttga
    15961 tccgacgggc aaattggcga gtatctttac gataattaaa gtcccaaaaa tcatcatttc
    16021 ttataaaata cagctcaaac tctcctgtac aggttttacc tccttggtct atacccgtaa
    16081 ttttaaactt attgtctttt gttgggtcta tattccaggt tccgcctgaa acatcgccat
    16141 cagcaagata ccggtgatat tgtacaataa caaaaggtga acctaccttc cagtccgagc
    16201 taagatacgc tgagtagtcc tttatcaaaa ctttatccac ctcaaaatcg aaggccaata
    16261 cttctcttgc aacatcggca tcctcaacaa atgcataata tggcgagctt gtgctcaatg
    16321 acaataaata cccatcacca tacttactat gccaaaggtt agcatcaaat cgccattcta
    16381 ctgagccggc acgtggcact gattttaacg cagttccacc tatacgatcc ttgtatttat
    16441 aatattgccg taagtcttcc gaaataaaag tatcttcctt attgcaagag gataataaca
    16501 aaatacctat atataaaata aataatttat gaatcttcat cattgcgtaa taattacttt
    16561 ttggattaca gaatttctaa tactatacaa tcttaaaaaa tacaaacctt gacttactcc
    16621 tctgaaatct acagcagcat ttctttcaat actattataa tctatagact taattaaatt
    16681 gccctttagg tcaaacaatt ccgctctgac tatagcttca ccatcaattt ccgcaatatt
    16741 tactaatccg gagcttgggt ttggatatac aagtcccata ttcgatacat ctgatattaa
    16801 atttgacgat cgaggacaat tttgatcaca tacaatgcgt tttttcgcaa aactgacata
    16861 accgtcagca gaagtaactt ttaggctaac ataaaatgcg ggacaattcg gtggctgcgg
    16921 tagataaata ctggatgtat tgcccaaata atttgatgga ttaaacaaac cattcaaact
    16981 ccagtaccat tcgtattgat aaggtccaac accataagga gcgacagata gtactgccgg
    17041 cggtgcgaaa aaaccatgct cgacatctgc atctaatgtc aatggcaatt ccgcgcaaac
    17101 tacccctgtt tctatttttg cttcaaaaat tgtaagattc ttattatctt caaccttaca
    17161 aaatgcccgg ctatcaatct gatgggcatt atctctgtaa tcagtttcac ctgttgacac
    17221 acccatatag cttacatcag gattcgaaaa ataaggaatt ctgccatagt ggggagctct
    17281 tgccatgatt gtgtacctgt aatagtttcc aaaatctaat actttcccat gagggcagcc
    17341 ggtagaattt tcaaaatcat tttcatgcaa acttccaaac tgatgtgcaa cttcatgtgc
    17401 aaaagtataa cgtatagggt caataaacgg tgtctctaca atacagaatt tatttaaagc
    17461 atcaggatca agtgaattcg ccgcaccata tataaccgct gatgtatcct tttttgatgt
    17521 atatgtacct ctgtctgtca ataaaacacc aatatcacat ttataataat taacaagata
    17581 tttggcattc gtacttattg ttaatgcatc caaatcatct tcaatatatt tcttagggtg
    17641 atacacaaaa tccggttcaa aatcgattgt ctttacccgt attctttttc cgccaacttc
    17701 gctgttaaac atcgctgtat taaaattatg cgtttcaaca aataaatatc agttggcata
    17761 tatcccccaa tggttatcca gccaaagtct tgcatccggt gtcaccatag ccaaaacatc
    17821 aacataagtt ttgtcgcaag tagtgccaca catagcaatt tcaccagata tcgctgcaaa
    17881 cgcgctatct gcaacggtac agtcaccggg tgtcattttt gttaaatcgt atttcatcaa
    17941 aacatgaaga cttgtaccga atggatataa ggcataaaca gtatttccta aatctatagt
    18001 tcctcctatg gcagtacttg ttttatacaa actcaaattg ccctgagggg cggatgctat
    18061 taaagtgaaa cttgtgtcac cgtcaaacag aatatcctga attttaaaag atacattacc
    18121 gtcaggtaga ttcatataaa catatccttg gtcttctatg tttggagata actgtaaata
    18181 atataatgta tcattataag gtagggaaga caaataatta aagatttttg tctgatctat
    18241 accaagtgtt ggaggtgggc ttgtatattg aaatacataa ttataagtct gtgcttcgag
    18301 cttcatcaac gacataatca cgagagcaag aattagaaac tttttatgta ctgatgtact
    18361 gatgtactga tgtactgatg tactgatgta ctgatgtact gatgt
//
LOCUS       DAOQQY010000022        18087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_954_length_18087_cov_7.183341, whole genome shotgun sequence.
ACCESSION   DAOQQY010000022 DAOQQY010000000
VERSION     DAOQQY010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 18087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18087
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_954_length_18087_cov_7.183341"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLE23_02380"
     CDS             complement(<1..443)
                     /locus_tag="PLE23_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX82142.1"
                     /translation="MIEVRNLRKNYGKLEALKGISFSIDEGEFYGLLGPNGAGKTTTI
                     SIISSILVPDGGEVSINGKNIYTHSRESKQIIGVVPQEIALYSDFSAMENLIFWGGLY
                     GVPDNELKRRAKELLQMVGLSDRANDKVSSYSGGMKRRINIAAAL"
     gene            complement(501..944)
                     /locus_tag="PLE23_02385"
     CDS             complement(501..944)
                     /locus_tag="PLE23_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMX82143.1"
                     /translation="MENIPSEDQTTPKVTGIGGIFFFSENPETTRNWYAKHLGLEVNE
                     WGSSFEFRNAHRPEEINYLQWSPFKKGSDYFAPSKKEFMINYRVQNIEGLVAKLKADG
                     VTVVDSIETFEYGKFVHIMDAEGNKIELWEPVDSVLTKLGTKTTK"
     gene            complement(1192..1596)
                     /locus_tag="PLE23_02390"
     CDS             complement(1192..1596)
                     /locus_tag="PLE23_02390"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMX82144.1"
                     /translation="MNFNGNAEEAFNFYKSVFGGEFSALVRFKDMEIPGFDVPEHEAN
                     KIMHIALPIGGNELLANDVPEMMGRVNEHENRSKIAVSVSSREEADQIYAGLTVGADI
                     EMPIGDSPWGGYFAMFRDRYGIEWTIDYDPRK"
     gene            complement(1715..2131)
                     /locus_tag="PLE23_02395"
     CDS             complement(1715..2131)
                     /locus_tag="PLE23_02395"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMX82145.1"
                     /translation="MVRTSVKIDAWIASDIKKVWHCWTQPEHITKWNFASEDWYCPSA
                     SNELTVGGKYTARMEARDGSFGFDFEAIYDEIVEHNKISYTMPDGRKVTTTFEADNGA
                     TNVTTVFDAESMNPVEMQQAGWQAILNNFKKYVEAN"
     gene            complement(2233..2772)
                     /locus_tag="PLE23_02400"
     CDS             complement(2233..2772)
                     /locus_tag="PLE23_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMX82146.1"
                     /translation="MNILKKILIAIAMLIAAVLLAALFIKKDYAVEKQIVINKPKQTV
                     FDYIKLLKNQDNYSKWAMMDPNMTKTYRGTDGTVGFVSAWDSKQENVGAGEQEIKKII
                     EGERIDLALRFTKPFEAHDDAYLVTEAVNDSTTNVKWGFTGAFPYPMNVMNLFMDMEK
                     AVGGDLQTGLENLKKVLEK"
     gene            complement(2984..3613)
                     /locus_tag="PLE23_02405"
     CDS             complement(2984..3613)
                     /locus_tag="PLE23_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMX82147.1"
                     /translation="MKKLVLLFAVSGLFLTSCVDNPQGDKAATTEAQQAPSDASGMAY
                     TLNPQESTLRWEGRKVSATHHGTVQFKSGKVQMSGNQLTGGEFAADMGTLVNEDLQGE
                     WNTKLVTHLKGADFFDIEKHPEATFQITGVKPGSSPNQVVVSGNLVIRGVSKNITFNA
                     DVVEAGDKKVQIKADFNIAREDWGITYTGMADDLISKEINFKINIVAYA"
     gene            3850..4914
                     /locus_tag="PLE23_02410"
     CDS             3850..4914
                     /locus_tag="PLE23_02410"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMX82148.1"
                     /translation="MGRQHFHTFDALRFSSFLLVFLHHLPKTGNIWIDFFLKSGGIGV
                     SFFFVLSGFLITYILLYEKKNRQTISLKKFFIRRILRIWPLFYLMIAFAFLSPYLLQL
                     FDIRSANNGYKPNLLISMLFVENYRMMLTDTFPDGAPLRIMWTLCIEEHFYLLWGILL
                     YFISVKNVPLLIFAAIVIANIARPIYSTVGIKHLDVFSNFDYFAFGALPAYILLFKNK
                     FMVAIDKIPVLTKHIFLLLTTCFVFIIPNVADLDTAYFWAPTLFCLLFSTLILFTLSK
                     NNIYIQDDLWYSRLGKYTYGLYLYHTIVLLLALHIFKPSSIISWCILTLISLIITCII
                     SSASYHLFEKQFLKLKRFYY"
     gene            5189..5638
                     /locus_tag="PLE23_02415"
     CDS             5189..5638
                     /locus_tag="PLE23_02415"
                     /inference="COORDINATES: protein motif:HMM:NF039391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HMX82149.1"
                     /translation="MRYLIFTCLCLVLPSGKLSKVAWLTGTWKHSSTDGAIYESWKKE
                     SENSLVGMSYTVKEGDTIVFETIHLTEQNDSLLYIPKVKYQNDEQPVIFTATIITNEK
                     MVFHNDQHDFPQIISYTKITTDSMVAEISGRAEGILKSKAYPMVRIE"
     gene            complement(5712..6218)
                     /locus_tag="PLE23_02420"
     CDS             complement(5712..6218)
                     /locus_tag="PLE23_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82150.1"
                     /translation="MVILLAGCNKADDPSVLIIDVSQQWSFDEQGYPIVGLLDGQWQS
                     KTFNKAEIALFNSLDTADLQNTSTPASVSDNPPDRNACFPNPFKDTHALYVGFNSDFS
                     SEVVVKAVIVDSTMTAKSKQVSRLKGQNGTLFKPNIPAGKYRLYYTLSSAANPHFYRT
                     WGNIYKKD"
     gene            complement(6476..8458)
                     /gene="gyrB"
                     /locus_tag="PLE23_02425"
     CDS             complement(6476..8458)
                     /gene="gyrB"
                     /locus_tag="PLE23_02425"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMX82151.1"
                     /translation="MEQNDDKKLSKQEKPNGNYDASNIQALEGLEAVRKRPGMYIGST
                     DTKGLHHLVYEVIDNSIDEHLAGYCSVIETIIHKDNSITVKDNGRGIPTGMHEKLQKS
                     ALEVVMTVLHAGGKFDKDTYKVSGGLHGVGVSCVNALSDYLKAEVHREGKIFVQEYSK
                     GIPQGPVETVGTTDNTGTYVTFKPDGTIFETLEYKFDTLHARLKELSYLNKGLELHLT
                     DERETEDDGSFKHKRYYSTGGLAEFVEFLDESRTKLTEEPIHVIDKEDNVEVEVAMQY
                     NTSFNENLISFVNNINTRDGGTHVQGFRRAINRVFKQYGDDNGLFKKLKFEIAGEDFR
                     EGLTAVVSVKVPEPQFKGQTKGELGNSEVTGIVSRCVSDKIKAYLEENPREARKIIDK
                     VILAATARHAARKAREMVQRKNVLTGSGLPGKLSDCSSKDPVESEIFLVEGDSAGGTA
                     KQGRDRMFQAILPLRGKILNVEKALEHKIYENEEIKNMFTALGVSIEENEDGDRFLNT
                     AKLRYHKVVIMCDADVDGSHITTLILTFFFRFMRELIDNDNIYIAAPPLYLVKKGKEQ
                     RYAWTEEERRKAATELAGEGKEDTAHVQRYKGLGEMNAEQLWETTMNPATRILRKVTI
                     EDAAESDRIFSMLMGDEVPPRRQFIEENARYAKVDV"
     gene            8622..9041
                     /gene="tsaE"
                     /locus_tag="PLE23_02430"
     CDS             8622..9041
                     /gene="tsaE"
                     /locus_tag="PLE23_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HMX82152.1"
                     /translation="MLKEWVFTLQGIDKIAKELLHSLPPDSKVFLSGEMGAGKTTLCK
                     SVIKNLGIDDEISSPTYSIVNEYIIPLSKRKLRHMDLYRLKNIDEALDIGIEDYLYDA
                     SFCLIEWPDIIENLANEKTYFLNISSMSDNRRKLVFL"
     gene            9057..10277
                     /locus_tag="PLE23_02435"
     CDS             9057..10277
                     /locus_tag="PLE23_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMX82153.1"
                     /translation="MSDPNIKIPIPASLMAGAVLTQEERLQVDQRKGKFVIGIPKEST
                     FQENRIALVPSSVAALIAKGYHIYIEAGAGEKANFSDRLYAESGATIIHSKEEVFKSD
                     VLIKVAPPTMDEIDLMHPGQILLSPLHLPMLTRDFFIKLKSKRVIAIAIEYIQDQVGS
                     FPVVRSMSEMAGISTILIGAELLSKSKGVLFAGISGVPPAKVCILGAGVVAENATRTA
                     LGLGAEVRIFDNNTYKLMRLQTHIGQKLFTSSFDPEVLDREIASSDLVIGAIHSKTGR
                     APIIITENMVSKMKPGSVIIDASIDQGGCFETSKITTHDDPTFEVYDVIHYCVPNISS
                     KVPQTASQGLSNILTPLLMQADDAGGIEKLLYTSFGLRHGVYTYKGSLTNPHLGDRFQ
                     INYTDLDLLITSNL"
     gene            10317..11015
                     /locus_tag="PLE23_02440"
     CDS             10317..11015
                     /locus_tag="PLE23_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HMX82154.1"
                     /translation="MKAGELIEHVVNLFYPKLCLACECENYSGTIPVCVSCLAELPLT
                     NMHNQQENLITERLLGRINLHSAAALFYFTKKSKVQHLVHQVKYSGDPTIANMLGHWM
                     GRYLSKSDLFNQIDMIVPVPLHKDKLQKRGYNQSAEFGKGLADILGSPCLPDALRRTK
                     QSVSQTTGNRTARLLNVHESFSLNTGIDLSGKHILLVDDVLTTGATIEACATKILALP
                     DTKISICVMAIVMD"
     gene            11127..12038
                     /locus_tag="PLE23_02445"
     CDS             11127..12038
                     /locus_tag="PLE23_02445"
                     /inference="COORDINATES: protein motif:HMM:NF018659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HMX82155.1"
                     /translation="MPAKFINNLHWSGSSPLIALLLLFFSKTIAVGQNIPFNFNNPDR
                     QIMLDSQLQEISGISMYKNDTTLLAIQDESGTVFTISGNTGAILKKTEFTGKGDFEDI
                     QYASPFVFANKSNGSIFQLDASTFKLIKSFESGLEKLNDVEGMFYSSTDNSLLLACKG
                     PKDQNPGSENARLVFRYDLKASRLIEKPIFHFTKDAFIEFVSKQQDTRENLDLKANYN
                     NNVPEFSFGPSAIAQDPITKNYYILSSAGKVLVVVKPNGEMVDIIKLHKKIYLQPEGL
                     SFDSKGNLYIASEGKKKVATLSFHKRL"
     gene            12115..13356
                     /gene="odhB"
                     /locus_tag="PLE23_02450"
     CDS             12115..13356
                     /gene="odhB"
                     /locus_tag="PLE23_02450"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HMX82156.1"
                     /translation="MSVVDIKVPSIGESITEVTLSKWLKADGDIVKVDEPICEFESDK
                     ATLEFPAEVSGKLTLIAKEGDDLKIGQLMARIDTSVSEPSATQATTSASAPQQTISTP
                     ENPTFSNVAGHPSPAAAKMMAESNIDASQITATGKDGRIRKEDVQVAQEKILTEEPKT
                     VVEAAPKAAEPTTSSPLTAFSRSVRKEKMTRMRRTIAARLVSAKNNTAMLTTFNEVDM
                     TALMDLRKNYQDRFVEKFGIKLGFMSLFAKACAKVLLEMPEVNARIEDDSLVFHDYAD
                     VSIAISTPTGLVVPPVHNTESLSLAEIEFKIKELADKARTGKLTLEEMTGGTFTITNG
                     GVFGSMMSTPILNEPQSAILGMHAIINRPVAVNNEMMIRPMMYIALSYDHRVIDGSSS
                     VTFLVRVKKLLEDPVSLLLDL"
     gene            complement(13366..14127)
                     /locus_tag="PLE23_02455"
     CDS             complement(13366..14127)
                     /locus_tag="PLE23_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMX82157.1"
                     /translation="MQNTATSYTIKLPQFEGPFDLLLFFIERDELDIYNIPIAKITDD
                     FLQYIKHIEKLNIDLASEFILVAATLMKIKAKLLLPRKAVDEEGNEIDPREELVQRLL
                     EYKQFKEILADIQKLEERRQLQEKRGNISQELHLIASRGLIDTEWESVTLFKLLKAFE
                     RVLENQRESKKIITHQVYNYPYTIAEQQEYILSIVQKDIKTGFDQLFKVCQNRIHAIV
                     TFLALLEMLNEGKIHIIVGEGFNNFWLSKVDQKAV"
     gene            complement(14310..14741)
                     /locus_tag="PLE23_02460"
     CDS             complement(14310..14741)
                     /locus_tag="PLE23_02460"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HMX82158.1"
                     /translation="MKSIQKLLVLSILLVGITSCEKESILPDHKIPAEIKSYVAAHFP
                     NCNIVRAVEENFELEKYEITLSCNVKLEFDKHKNIIDIDGISKLPDSVIPAPILSYLG
                     ANFPSNTITGWETELNIQKIELDNDVTLVFNQKGEFLYIED"
     gene            complement(14774..15223)
                     /locus_tag="PLE23_02465"
     CDS             complement(14774..15223)
                     /locus_tag="PLE23_02465"
                     /inference="COORDINATES: protein motif:HMM:NF022838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HMX82159.1"
                     /translation="MKKIILFAMFASSVSFSGIFAQEISQRNVPSVILNKFQQEYPKA
                     LDIDWKKEADLYKVDFEIGSRRTDHDVWYNNEGKVVKHKKEIAKRDLPAAVLAKIKAD
                     FSGYRISDIKQITEAGKSIYTLELNKLTKEWKMTFDSTGNILDQIAD"
     gene            complement(15310..16587)
                     /locus_tag="PLE23_02470"
     CDS             complement(15310..16587)
                     /locus_tag="PLE23_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMX82160.1"
                     /translation="MKLLNQTLRYISIATLIIISIWAFIFYFIILREIKNSIDEGLDN
                     YKRQIIYNFQKKSGIETKTSFDEGFYTVEPISKHSAFSIRDTYTDTLLMMQDADDTSP
                     EPEPVRMLTTSFEMDGSYYQMRIINSMVEEDDLVKAMFWYFVWLYLILIAGIIFTNNY
                     ALQRLWRPFYRLIQQLTNYNILKSDSFPEVKTRTTEFRDLQNAVRTLLAHNAETFEKQ
                     KQFISHASHELQTPLAIAINKLELLLEDENLSNKQTGHIADTIRTIERLSTVNKSLLL
                     LSKIENKQFFDNRPVCINDIILQSVEEFEEFAAYKNVKISIVASDKLMVVMDESLARI
                     LVSNLLRNAIFHNIAGGKVFIEIKPDSLSFKNNGLRQALDSQRVFEAFYKDAANTSST
                     GLGLAILKSVCHLYRFNVNYRFEEELHVFEINF"
     gene            complement(16584..17261)
                     /locus_tag="PLE23_02475"
     CDS             complement(16584..17261)
                     /locus_tag="PLE23_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX82161.1"
                     /translation="MKILIIEDEPELSKVISESLAKEHFIIETAVDYNTASEKVFVYD
                     YDCILLDIMLPGGTGIDILNELKKTGKDANIIIISAKDSLEDKLKGLSLGADDYLTKP
                     FHLAELNARVHAVLRRKKLEGKNTLEYGNLTLDIERRQVSINDKTINLNRKEFDILEY
                     FLLNKSRLVTRTALAEHVWGDNIDQADNFDFIYYQIKNLRKKLQESDATITIEAIYGV
                     GYKLKEQ"
     gene            17388..>18087
                     /locus_tag="PLE23_02480"
     CDS             17388..>18087
                     /locus_tag="PLE23_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMX82162.1"
                     /translation="MPAGKLYMIPSALSEHSVYTPGRHELEVIHSLRLFLAERAKTAR
                     HYIATTLPPYKQQEIDVLELPKHGDDGTVKDFIKRIQSGESGGFLSEAGCPGIADPGA
                     EVVSQAYSAGIEVVPLVGPSSILLALMASGMSGQHFCFKGYLPQDKTKLAQVLVSMEA
                     ESARTDCTQIWIETPYRNNAMREVCLKTLKAGTRFCIAANLTGPQERIFSAKIKNWQP
                     TKLGDLQKTPAIFLM"
BASE COUNT         5237 a         3789 c         3454 g         5607 t
ORIGIN      
        1 aataatgctg ccgctatatt gatacggcgt ttcataccac cagagtagct gcttacctta
       61 tcgtttgccc tatccgacag gccgaccatt tgcaggagtt ctttggcacg tctttttagc
      121 tcattatcag gcacaccata gagtccgccc cagaatatca ggttttccat tgccgaaaaa
      181 tcactgtaca gtgctatttc ctgtggcact acaccaatta tctgtttact ctcgcggcta
      241 tgtgtgtaga tattcttacc attgatgctc acttctccac catccggcac gagtatggag
      301 cttataatgg agatggtggt cgttttgcca gcaccgttgg gcccgagcag accgtagaat
      361 tcaccttcat cgatagaaaa tgatattccc ttgagtgctt cgagcttacc gtaatttttc
      421 cttaggtttc ttacttctat cattcactgt gttggttgaa tacttatata aataaatgct
      481 gtgacaatgg ccgtgcaata tcatttggtc gtcttcgttc caagttttgt cagtacgctg
      541 tccaccggtt cccatagttc aattttattg ccctctgcat ccataatatg gacgaatttt
      601 ccgtactcga atgtctcgat gctgtccacg acagtgacac catcagcctt gagtttagct
      661 accaggcctt ctatattctg cacgcggtag ttgatcataa attctttctt tgaaggcgca
      721 aaatagtcac tgcctttttt gaaaggactc cactgcaggt agttgatttc ttccgggcga
      781 tgtgcattcc taaactcaaa tgatgagccc cactcattca cttcaagacc cagatgcttt
      841 gcataccaat ttctggttgt ttcaggattt tcggagaaaa agaatatgcc accaatgccg
      901 gttacttttg gtgtagtttg atcttctgaa ggaatatttt ccatatttat attatttttt
      961 gtcagagatg tatgggaggt gaatgtgcaa gctgaaatgg atactgctac gaagctacag
     1021 acaacccaga tcagggaatt ctttatattt ttcatcattc ttagggcgaa tacttaaatt
     1081 tttcaataaa aataatggct acgaaacgca atttgtaaca caacaatctt atttctacag
     1141 aattctaata taaaccatcc tgacatcaag ccgtgaatac aatatgcgcg tttatttccg
     1201 cgggtcgtag tcaatggtcc attcgatacc atatcgatct ctgaacatgg caaaataccc
     1261 tccccaaggg ctatcaccga tgggcatttc tatatcagca cctactgtga gtccggcgta
     1321 aatttggtcg gcttcttcgc ggctactaac actcacagct atcttgctgc ggttttcgtg
     1381 ttcatttacc cggcccatca tctccggcac atcattagca agtagttcat tgccgcctat
     1441 tggcaaggca atgtgcatga tcttattggc ctcgtgctcc ggtacatcaa agcccggtat
     1501 ttccatatcc ttaaacctca ctaaagcgct gaactctcca ccaaaaactg atttataaaa
     1561 attaaaggcc tcttcagcat taccgttaaa attgatctat ggattgattt ttaccatcgt
     1621 agattatgat tgttgttaat gaattctgtt aacgtgctga acattaattt cccccaaaaa
     1681 agcagccttt atgcaagtaa actagaccaa gggttcagtt tgcttcaaca tactttttaa
     1741 aattattgag tatggcctgc caccctgcct gctgcatttc taccggattc atactttcag
     1801 catcaaagac tgttgtcaca tttgttgcgc cattatctgc ttcaaatgtt gtagtcacct
     1861 ttctgccatc gggcatggtg tagctaattt tattgtgttc gactatctca tcatatattg
     1921 cttcgaagtc aaaaccgaag ctaccgtcac gtgcctccat tctggcagta tatttaccac
     1981 caacggttaa ctcattggat gctgaggggc agtaccagtc ttcggatgca aaattccact
     2041 tcgttatgtg ttccggttgt gtccaacagt gccacacttt tttgatatca gaggctatcc
     2101 aggcatctat ttttactgaa gttcttacca ttttataatt gtgtttgttt ttgtttgaaa
     2161 ttgggtttac taccattcat caagatagag gctgatgatt gaaatattca gcgtatttca
     2221 gtatcaataa gactattttt caagtacttt tttaagattt tcaaggccgg tctgcagatc
     2281 gccgcctaca gctttttcca tatccatgaa caggttcatc acattcatcg gataagggaa
     2341 ggcacctgta aagccccact tgacattcgt ggtgctgtca ttcacagcct ctgttaccaa
     2401 ataggcatcg tcatgcgctt cgaatggttt ggtgaatctg agtgccagat ctattctttc
     2461 gccttctatg atttttttga tttcctgctc accagctcct acattttcct gtttgctgtc
     2521 ccaggcagat acgaatccga cagtgccatc cgtgccgcga taagttttgg tcatgttggg
     2581 gtccatcatt gcccacttac tgtagttgtc ttgattcttt agcagtttga tataatcgaa
     2641 taccgtctgt tttggcttat tgatgactat ctgcttttct accgcataat cttttttaat
     2701 aaataatgca gcgagcagta cagcagctat tagcatagca atagcgatga gaatcttctt
     2761 aaggatattc atattgttgg ttttaaatga ttaatcggta aaattaataa tttttccaaa
     2821 ccaacaaaat ttttatctgc aatattttag tacagtcaaa atagaattag gttaattctg
     2881 tgtaaatatt cgtcaatatc aggtactgac aaggcgcaca cgtaacaaaa aaagggaaag
     2941 cgccggcctt cccttttata gaattatatt gtgtccatta gatttaggcg tatgctacga
     3001 tgttgatctt gaagttgatt tcctttgata taaggtcatc ggccatacca gtgtaagtaa
     3061 ttccccagtc ctctctagca atattgaaat cagcctttat ctgtactttc ttgtcaccag
     3121 cctctactac atcagcattg aaagtaatat ttttcgaaac acctcttatc acaaggttac
     3181 cagacactac tacctgattc ggactgctgc ccggctttac tccggttatc tgaaatgtag
     3241 cttccggatg tttttcgata tcaaagaagt cagctccctt aaggtgtgtt actagtttag
     3301 tattccactc gccctgaagg tcttcgttca ctaaggtgcc catatcagct gcgaattcgc
     3361 caccggtgag ttgattacct gacatctgta ctttgcctga cttaaactga accgtaccat
     3421 ggtgtgtagc ggatactttg cgaccttccc agcgtagagt tgattcctgt ggatttagtg
     3481 tgtaggccat tccgctagca tcagatggtg cctgctgtgc ttcagtagta gcagccttat
     3541 cgccctgcgg attgtcaacg catgatgtaa gaaaaagacc cgatacagca aataataaaa
     3601 ctaatttttt catctttcgt gtttattgtt ttatggttga ttaaataatt tactgcaaag
     3661 ttaggccgat acaaaagtgt ttttgcttaa tctactttaa gaaaaccgga catttgacca
     3721 aattaatgtt aaatagtgtt aaataagcca tcatattata gaaaaatatt tcttatcttt
     3781 ggaaatatgt atagtaccgg ccggattcgt tttgacataa taattatgac aatccaataa
     3841 aaccacaaca tgggcagaca acatttccat acattcgatg ctctgaggtt ttcttccttc
     3901 ctcctggttt tcttgcatca tttacccaaa accggtaata tttggataga ttttttcctc
     3961 aaaagcggag gtattggggt cagttttttc tttgtgttga gtggctttct gattacttat
     4021 attttattgt acgaaaagaa aaaccggcag accatttcat tgaaaaaatt ttttatccgc
     4081 cgtatattgc gcatctggcc tttgttttat ctcatgatag cattcgcctt tctgtcacca
     4141 tatttgcttc aactctttga tattcgttct gccaacaatg gatacaagcc gaatctctta
     4201 atatcgatgc tgtttgtcga aaattacaga atgatgctaa ccgacacatt tcctgacgga
     4261 gcgccattaa ggatcatgtg gacactctgt atagaagaac atttttattt gctttgggga
     4321 atattgctct atttcatttc agtaaaaaat gttcccctac tcattttcgc agcaattgtc
     4381 attgccaaca tcgcaagacc tatatacagc acagttggta taaaacatct cgatgtgttt
     4441 tcaaatttcg actattttgc attcggagcg ttaccggcat atatactttt atttaaaaat
     4501 aagtttatgg tagccatcga caaaatacct gttttgacaa aacacatctt tctattgctt
     4561 acaacatgtt ttgttttcat tattccgaat gttgcagacc ttgatactgc gtatttttgg
     4621 gcaccaactc ttttctgcct gctgttctct acactcattc ttttcacctt aagcaaaaac
     4681 aacatttata ttcaagacga tttgtggtat agcagattgg gcaaatatac ctatggttta
     4741 tatttatacc atactatagt actgctgctg gcactccata tttttaagcc ctcttctatc
     4801 attagctggt gtatccttac gttgatctcc cttatcatca cctgtattat cagcagcgct
     4861 tcatatcatt tgttcgaaaa gcagttttta aaacttaaaa gattttatta ttgaacatac
     4921 cacatataat gtatggttac agctttggat gttaacacta cccatataaa atattattaa
     4981 caacagacca aagtactgta cttttatctt gatcttaccg aaataatcaa atactgctgc
     5041 ttatctttac cccagcttac acaaaattaa tttcaacgaa aatgattagt acaaaaaatc
     5101 aatgaaaaag tgataaaagc ataataaact gtgattttga gcttttgcaa tggatttgaa
     5161 gtaataatta tttgcagtaa agagtctaat gcgatatttg attttcactt gtttgtgtct
     5221 ggttttacct tcaggaaaac tttcgaaagt tgcatggctg acaggtactt ggaaacatag
     5281 ttctacggat ggagcaatat acgaaagctg gaaaaaagaa agcgagaact cgcttgtcgg
     5341 catgagctat acggtcaagg aaggtgatac aatagtgttc gaaacgatac atttaacaga
     5401 acaaaatgac agcctattat atataccaaa ggtaaaatac caaaatgatg aacagcctgt
     5461 aatcttcacg gccaccatta ttacaaatga aaaaatggtt ttccacaatg atcaacatga
     5521 ttttccgcag atcatttctt acactaaaat aacaacagat tcaatggtag ctgaaatttc
     5581 aggcagagca gaaggtattc taaaatctaa ggcatacccg atggtgagga tagaatagtc
     5641 ttcaaaaaaa tttgttaaac ccagacatac tgaaaaagat attagttgcg ggttcagtag
     5701 gatcggaagg cttaatcttt tttataaata ttgccccagg tcctataaaa atgtgggtta
     5761 gccgcagaac tcaaggtata atacaagcga tatttgccgg caggtatatt tggtttaaat
     5821 aatgttccgt tttgtccttt aagtcgggat acttgctttg atttggccgt cattgtacta
     5881 tctactatta ctgccttgac aactacttcg ctagagaaat cactattaaa tccaacatac
     5941 aaggcatgtg tatccttaaa tggattggga aagcaagcgt ttctatccgg tggattgtct
     6001 gacaccgaag ctggtgtgct tgtgttttga aggtcagccg tatcaagact attgaacaat
     6061 gcaatctccg ctttattaaa ggtttttgac tgccactgac catcaagcag accaactata
     6121 ggataaccct gttcatcaaa actccattgc tgggaaacat caattatcaa aactgacgga
     6181 tcatcggctt tattacaacc tgccaacaat attaccaata gaagaaaatt taataatcta
     6241 gttatcacgt tcgatttaat tataaaccaa caattaacaa acaacaaatg cacaaaaacg
     6301 tattcgctat cgcaaatata agaaaattca tcaggtaaaa tccacaccaa cattatgcca
     6361 cgatgggcac accgacattg tccctcaatg tatccatatg ataaaaaacc ttacacagac
     6421 aaattcattt tgcatcgggt ttcactcaag tactgagttg ggtgcaaaat gatgactata
     6481 catctacctt tgcatatcgt gcgttttcct ctataaactg tcttcttgga ggcacttcgt
     6541 cacccattag catggaaaat attctgtcac tctcagccgc atcctcaata gtaacttttc
     6601 tgagaatcct cgtcgccggg ttcatggttg tctcccaaag ttgttcggca ttcatttcac
     6661 ccagaccttt gtatcgttgt acgtgagcag tatcttcttt tccttcaccg gctaattctg
     6721 tagctgcctt ccttctttct tcttctgtcc atgcataacg ttgctctttg cctttcttta
     6781 ccaagtacag tggtggtgct gcaatgtaga tattgtcatt atcaataagc tcacgcataa
     6841 acctgaagaa aaaagtaaga atcaaagtgg taatgtggct accgtcaacg tcggcgtcac
     6901 acatgatcac tactttatga tagcgtagtt tggcagtgtt caggaatcgg tcaccgtctt
     6961 cgttctcttc aatactgaca cccaatgccg taaacatatt cttgatctct tcattttcat
     7021 agattttgtg ttcgagtgct ttctcgacat tcaggatctt acctctgagt ggcaatatag
     7081 cttggaacat acggtcacgc ccttgcttgg cagtaccacc tgccgaatct ccctccacca
     7141 gaaatatctc tgactctacc ggatcctttg acgagcaatc tgaaagcttg cccggaagtc
     7201 cgctaccggt cagtacgttt ttgcgctgta ccatttcacg tgccttacga gctgcatgcc
     7261 tggctgtagc cgccaatata actttatcta ttatcttcct tgcttcacgt gggttttctt
     7321 ccagataggc tttgatcttg tctgatacac atcttgaaac aatacccgtc acctccgagt
     7381 tgccaagttc gcctttggtc tgacctttga actgtggctc aggtactttc acagatacga
     7441 cggctgtaag cccttcgcga aagtcttcac ctgcaatctc aaacttcagc ttcttaaaca
     7501 aaccgttatc atcaccatat tgtttgaata cacggttgat ggctcgacga aacccctgta
     7561 cgtgtgtacc tccgtcgcga gtattgatgt tattaacgaa cgatatcagg ttttcgttaa
     7621 atgaagtgtt gtattgcatg gccacctcta cctctacatt gtcttccttg tcaatgacat
     7681 ggattggctc ttcggtgagt ttggtacgcg attcatccag gaattcgaca aattctgcaa
     7741 gaccaccagt cgaataatat cttttatgct taaaactgcc atcgtcttct gtttcgcgtt
     7801 catctgtcag gtgtagctcg agacctttgt tcaggtacga aagttctttt aacctggcat
     7861 gcagtgtgtc aaatttgtat tccagcgttt cgaagatggt tccgtcaggt ttaaatgtca
     7921 cataggtacc cgtgttgtcg gtagtgccca cagtctcaac agggccttgt ggaatgccct
     7981 tcgagtattc ctgaacaaaa attttccctt cgcggtgcac ttctgccttc aaatagtctg
     8041 acaaggcatt gacacaagat acaccaacac cgtgaagacc accggaaacc ttataggtat
     8101 ctttgtcaaa tttgccgccg gcatgcaata ccgtcataac tacttcgaga gccgatttct
     8161 gtaacttttc gtgcataccg gtaggaatac ctcggccatt atcttttaca gttatcgaat
     8221 tatccttgtg gatgatggtc tctattaccg aacaatatcc ggcgaggtgt tcatctatag
     8281 aattgtcaat tacttcataa actaagtggt gaagaccttt agtatcagtg ctaccgatgt
     8341 acataccagg tctttttctt actgcttcca gaccttcaag tgcctggatg ttactggcat
     8401 catagttgcc atttggtttt tcttgtttgc tcaatttttt atcatcattt tgttccatac
     8461 cgcaaatata caaaaaatac tgtaatttta catttttaac atttatttat taaaattaat
     8521 atattgttaa tcaattattt atataaaaaa tatttataaa attgaaggca aatattattt
     8581 tgaaataagc tctaccaaca aagaataatc caaattttat tatgctaaag gaatgggtat
     8641 ttacattgca gggaattgac aagatagcca aagaattgct ccattctctg ccacccgaca
     8701 gtaaggtgtt tttgtcaggt gaaatgggtg caggcaaaac taccttgtgc aaatctgtaa
     8761 taaaaaattt gggtattgat gatgaaatat caagtcctac ctactctatt gtcaatgaat
     8821 atataatacc tctgtcaaag cgaaagctaa ggcacatgga tctctacagg cttaaaaata
     8881 tagacgaagc gcttgatatt ggaatagaag attatctgta tgatgcttcg ttttgtctca
     8941 tcgaatggcc cgatataatc gaaaatctgg ccaacgaaaa aacttacttc ttaaatataa
     9001 gcagcatgtc ggataatcgt cgtaaattgg tatttttgta gcgaaataca atacttatga
     9061 gcgacccgaa tatcaaaatt cctattccgg caagcctgat ggcaggtgca gtacttaccc
     9121 aagaagaacg cctgcaagta gatcagcgaa agggaaaatt tgtcatcggg attcctaagg
     9181 aaagtacatt tcaggagaac agaatagcac tcgtaccatc atctgtagca gcgcttattg
     9241 ccaaaggata tcacatctac atagaagcag gtgcaggcga aaaagcaaat ttttctgaca
     9301 gactctatgc agaaagcggc gcaaccatca tccatagcaa agaggaagta tttaaatcgg
     9361 atgtactcat caaagtagca ccgcctacta tggacgaaat agacctgatg caccccggac
     9421 aaattctgtt gtcgccccta catctcccta tgctgacaag agattttttt ataaaattaa
     9481 aatcaaaacg agttatagcc attgccatag aatacatcca agatcaggta ggttcatttc
     9541 ctgtcgtcag atcgatgagc gaaatggccg gtataagcac aatactgata ggtgccgagc
     9601 tattgagcaa gagtaaaggt gtactgtttg caggtatatc gggtgtacct cctgcaaaag
     9661 tctgtatatt gggagcaggt gttgtagccg aaaatgccac acgtaccgca cttggcctcg
     9721 gcgcagaggt ccggatattc gacaacaata cttataaact gatgaggttg cagacacaca
     9781 tcgggcaaaa actgtttact tcatccttcg acccggaagt attggacagg gaaattgcaa
     9841 gttctgacct cgtcattggt gccattcatt ctaaaaccgg ccgtgcaccc ataatcatta
     9901 cagaaaatat ggttagcaaa atgaaacctg gctctgttat tattgatgcg agtattgacc
     9961 aaggaggctg cttcgagaca tccaaaataa caacccatga cgatcctacc ttcgaagtat
    10021 atgatgtcat ccattactgc gtaccgaaca tttcatccaa agtgccccag actgcttcac
    10081 aaggcctttc caatattctt acaccattat tgatgcaggc tgatgatgca ggcggaattg
    10141 aaaaattgct ttacacaagc ttcggactac ggcacggtgt ctatacatac aaaggaagcc
    10201 tgaccaatcc tcatctcggt gatagatttc agatcaatta taccgacctc gacctgctga
    10261 tcacatccaa tctctgatgg tttccatgat acatagtcag cataaatgta caaagaatga
    10321 aagccggcga attaattgag catgtagtca acttatttta cccaaagctt tgccttgcct
    10381 gtgaatgcga aaactacagt ggtacaatac cggtatgtgt atcttgcttg gcagagctgc
    10441 cactcaccaa tatgcacaac caacaagaaa atctgataac ggaaagatta cttggcagga
    10501 tcaatctgca ctcagctgct gcattatttt attttacaaa aaaaagcaaa gtgcagcacc
    10561 ttgtccatca ggtaaaatat tcgggcgacc caacaatcgc aaatatgctc ggacattgga
    10621 tgggcaggta cctgagtaaa tctgatctgt tcaaccagat agatatgata gtaccggtac
    10681 ctttgcacaa agacaaatta caaaagaggg gctacaacca aagtgcagaa ttcgggaaag
    10741 gcttagcaga catactgggt agtccatgcc tgcccgatgc acttcggcgc actaaacaat
    10801 cggtctccca gaccaccggc aacaggaccg cacgactctt gaatgtgcat gaatcttttt
    10861 cccttaatac cggaattgac ctgtcaggca aacacatcct gctcgtagat gatgtactca
    10921 ccacaggagc caccatcgaa gcctgtgcta ccaagattct tgcattgccc gataccaaaa
    10981 taagcatctg tgtaatggcc attgtaatgg attgataaac ggttatacac cggaaatcat
    11041 tttataatat ttaacacaag tttttatcca caatacacat ttttatccat ttctttgcgt
    11101 tttaacttgt aacaaaacaa catataatgc ctgctaaatt catcaacaac ttacattggt
    11161 caggaagcag tccgctgatt gctttgctat tgcttttttt ctccaaaacc attgctgttg
    11221 gacagaatat tccatttaat tttaacaatc ctgacaggca aattatgctc gacagtcagt
    11281 tgcaggagat ttcgggtatc agtatgtaca aaaatgatac aaccttactt gctatacaag
    11341 acgagtcggg tacagtgttt accataagtg gcaatacagg tgccatcttg aaaaaaacag
    11401 aatttacagg aaaaggcgat tttgaagata tacagtatgc ttcaccattt gtatttgcca
    11461 ataaaagcaa tggcagcatt tttcagcttg atgcctcaac atttaagctc ataaaatctt
    11521 ttgaatccgg ccttgaaaaa ctcaatgacg tagaaggaat gttttattcg tcaaccgaca
    11581 atagccttct actcgcatgc aagggaccaa aagatcaaaa tccgggttcg gaaaatgccc
    11641 gattggtctt ccgctacgat ctgaaggcaa gcagactaat agaaaaaccc attttccatt
    11701 tcactaaaga tgcatttata gagtttgtaa gcaagcaaca ggacacaaga gaaaacctcg
    11761 acctcaaggc aaactacaat aataacgttc ccgaattttc attcggccct tcggccatag
    11821 cacaagatcc tattaccaaa aattattaca ttctgtcatc agccggaaaa gtgctggtag
    11881 tagtaaaacc taacggcgaa atggtagata ttattaagct gcacaagaaa atatatctac
    11941 agccagaagg tttaagtttt gactcaaagg gcaatcttta tatagcaagt gaaggtaaaa
    12001 aaaaggtggc aacgctgagt tttcataaaa gattgtaata aactacattt cagctctttt
    12061 attttaacta attttgctcc cgttcgttta gttaattagc aatttatttt tgaaatgagt
    12121 gtagtagata ttaaagtacc atccatcggt gaatcaataa cggaagtaac cttgtcaaaa
    12181 tggctgaagg ccgacggtga tatcgtaaaa gtagatgaac caatctgcga atttgaatct
    12241 gataaagcaa cgctggaatt tccagccgaa gtttcgggaa aattaaccct gatagctaag
    12301 gaaggcgatg acctgaaaat tggtcagctc atggcaagaa tagatacatc tgtttctgaa
    12361 ccttcagcta ctcaggctac aacctcagct tcagctcctc aacagaccat ttctactcct
    12421 gaaaacccaa cattctcaaa cgtagcaggt catccttctc cggctgctgc caaaatgatg
    12481 gctgaatcaa atattgatgc atcacagatt actgctaccg gtaaggatgg tcgcattcgg
    12541 aaggaagatg tacaggtagc tcaggaaaaa atattaactg aagaaccaaa aactgttgta
    12601 gaagcggcac ctaaggctgc cgaaccgaca acatccagcc ctttgaccgc attcagtcgc
    12661 tcagtacgta aagaaaaaat gaccagaatg aggcgaacca ttgctgcccg cttggtatca
    12721 gccaagaaca atacggccat gctcaccacc ttcaacgaag tggacatgac agcactcatg
    12781 gacttgcgca aaaactacca ggacagattt gtcgaaaaat tcggcatcaa actgggtttt
    12841 atgtcactct tcgcaaaagc ttgtgccaaa gtattactcg aaatgcccga ggtgaatgcc
    12901 cgtatcgaag atgattctct agtattccac gactatgcag atgtatcaat agccatttct
    12961 accccgaccg gacttgtagt accacctgta cacaatacag aatcactcag cctggcagaa
    13021 atagaattta aaattaaaga attagctgat aaggcacgta ccggcaaact cacactcgaa
    13081 gaaatgacag gcggtacatt taccatcacc aacggtggtg tgtttggctc tatgatgagc
    13141 acaccgatac tcaacgaacc tcagtcggcc atattgggta tgcacgctat catcaacagg
    13201 ccggtagcag tcaacaacga aatgatgata agacctatga tgtacatcgc tttgtcttac
    13261 gatcaccggg ttattgacgg cagttcgtct gttacgtttt tggtaagagt gaaaaaattg
    13321 ctcgaagatc cggtcagtct cttattagat ttgtaaaata aaaaatcaca cagccttttg
    13381 atctaccttt gacaaccaga agttgttgaa accttcacct acgatgatgt gtattttacc
    13441 ttcgttgagc atctcaagca atgccaggaa ggtcacaata gcatgtatgc ggttttggca
    13501 cactttaaac aactgatcga atccggtttt gatatctttt tgcacgatgc tgagtatgta
    13561 ttcctgctgt tcggctatgg tataagggta gttgtacacc tggtgcgtga tgatcttctt
    13621 tgattcgcgc tggttttcaa gtactctttc gaatgctttg agtagtttga acaaagtgac
    13681 ggactcccat tctgtatcaa tgaggcctct tgaagcaatg aggtgtaatt cctgacttat
    13741 gttgccgcgt ttttcctgta gttgccgtct ctcttccaac ttttggatgt cagcaagtat
    13801 ttccttaaac tgtttgtact caagtagcct ttgtacgagt tcctctcttg ggtctatttc
    13861 gttgccttct tcatctacgg ctttccttgg cagcagtagt ttggctttaa ttttcattag
    13921 tgtagctgct acaagtataa attcgcttgc caggtcaata tttagctttt caatatgctt
    13981 gatatattgg aggaaatcat cggttatttt agcaatggga atgttgtaaa tatccagctc
    14041 atcgcgttct atgaaaaaca aaagcagatc gaacggacct tcgaactgtg gtagtttaat
    14101 agtatagctt gtagctgtat tctgcatata ctgcaaagtt aatgaatgtt agtgagccaa
    14161 tactatgagg ctacaatatt tcagatgtta ttgcgtgttt cttatgattg tctgaataaa
    14221 aaattatccg gaacaaatac atacgaaaag gtggttgctc ttacctgaat tttagaacaa
    14281 ccaccctttc attctggtta acctaccggt caatcttcga tgtacaaaaa ttctcctttc
    14341 tggttaaata caagggttac atcgttatcc agttcaattt tctgaatgtt aagctctgtt
    14401 tcccaaccgg taatggtatt tgaaggaaag tttgcgccta aataggaaag tattggagca
    14461 ggtatcacac tgtccggcaa tttagaaata ccgtcaatgt caatgatatt tttatgcttg
    14521 tcaaattcga gtttgacatt gcatgacagt gtaatttcgt atttctcaag ttcaaaattt
    14581 tcctccactg cccttacaat attgcaatta gggaaatgtg cagctacata gcttttgatt
    14641 tctgccggta ttttgtggtc gggtagaata ctttccttct cgcagcttgt aatgcctacc
    14701 aaaagaatac tcaggaccaa caatttttga atgcttttca tgatattttt gtttatttga
    14761 atgattggaa atgctaatcg gctatttggt ccaaaatatt gcccgtggaa tcaaaagtca
    14821 ttttccactc tttggtcagc ttgttgagtt caagtgtata tatcgactta ccggcttctg
    14881 ttatttgctt gatatcgctg atgcggtatc ccgaaaaatc tgctttaatc tttgccagta
    14941 cggcagccgg aagatcgcgt tttgcgattt ccttcttgtg tttcactact ttaccttcat
    15001 tattatacca gacatcatgg tcagtgcggc gagaaccaat ctcgaaatct accttgtaga
    15061 gatcagcttc ctttttccag tctatatcca gtgcctttgg atactcttgt tgaaacttgt
    15121 tgagaataac agaaggcaca tttctttgac taatttcttg tgcaaaaatg cctgagaaag
    15181 acacggagga agcgaacatt gcaaataaaa tgattttttt catgtttttg acttttttta
    15241 aattttaata caaaggaatg aaccgattct ggaaagattc tggaatcaat aacaaccatg
    15301 tctgattttt taaaaattta tttcaaaaac atgcaattct tcttcgaaac gataatttac
    15361 attgaaacga tacagatggc acaccgactt aaggatagcc aaaccaaggc ctgtactact
    15421 tgtattggca gcatccttgt agaaggcttc aaaaaccctt tggctgtcaa gtgcttgtct
    15481 gaggccatta tttttaaagc tcaggctatc gggttttatt tctatgaata ctttgccgcc
    15541 ggcaatatta tgaaaaatag cattgcgcaa caaattggag acaagaatgc gggcaaggga
    15601 ttcgtccatc actaccatga gtttgtctga cgctacaatg gaaattttta cattcttata
    15661 ggctgcaaat tcttcaaact cttcaacaga ctgtagaatg atatcattga tacacaccgg
    15721 tctgttgtca aagaattgtt tgttttcgat tttacttaac aacaacaacg atttattgac
    15781 agtactgagc cgctcgatag tacgaatagt atcagcaatg tgtccggtct gtttgttgct
    15841 caggttttca tcctcgagca ggagttcgag tttgttgatg gctatggcta gcggagtctg
    15901 tagttcgtgc gaggcgtggc tgatgaattg cttttgcttt tcgaatgtct cggcattatg
    15961 cgcaagtaat gttctgacag cattttgaag atctcgaaat tccgtggtac gtgtctttac
    16021 ctccgggaat gaatcagact tcaggatatt gtaatttgtt aattgttgga ttagtctgta
    16081 aaaaggacgc cacagccgtt gtaatgcata attgttggta aaaataatgc ctgctattaa
    16141 aataagatac agccagacaa aataccaaaa catcgctttc acgagatcat cttcctcgac
    16201 catagagttg atgatacgca tttggtagta gctgccatcc atctcgaacg aagtggtgag
    16261 cattcgtacc ggttcgggtt cgggtgaagt gtcgtctgca tcctgcatca tcaacaaggt
    16321 atcagtatag gtatctcgta tgctgaatgc tgaatgtttg ctgataggct ctactgtata
    16381 aaagccttcg tcaaagctgg ttttggtttc aataccggat ttcttttgaa agttatatat
    16441 gatttgcctt ttgtagttgt caagtccttc atcaatgctg tttttgattt ctcttaatat
    16501 gataaaataa aaaatgaatg cccagatact gataataata agtgtggcta ttgaaatata
    16561 cctgagtgtt tggttgagca gtttcattgt tcctttagtt tatacccaac accatatatt
    16621 gcttcgatgg tgatagtagc atcagattcc tgcaatttct tcctcagatt ttttatctga
    16681 taatatatga agtcgaaatt atctgcctga tcgatattgt caccccagac atgttcagca
    16741 agggcagttc tggtcaccag tctgcttttg ttcaagagaa aatattcgag aatatcaaat
    16801 tctttgcggt taagattgat tgttttatca ttgatactta cttgtcttcg ttctatatca
    16861 agtgtaagat ttccgtattc gagcgtattt ttcccttcca atttctttct tcgcaacaca
    16921 gcatgtaccc tggcattcaa ttctgccaga tgaaaaggct tcgtcagata atcatctgca
    16981 ccaagtgaga gtcctttgag tttatcttcg agtgaatcct tggccgaaat gatgataatg
    17041 ttggcatctt ttcctgtttt tttgagttcg ttgagtatat ctatgccggt accgccgggt
    17101 aacataatat cgagaaggat acagtcataa tcatatacaa acactttttc ggaagcggta
    17161 ttgtaatcaa cggctgtttc gatgatgaaa tgttcctttg ccaaagactc cgagataacc
    17221 ttacttagtt cgggttcatc ttctattatt agtattttca tggttaaaga tagtaaatga
    17281 attctggaaa aatttaggaa tttttattaa aatgatagga tttggagcca tccaatcaaa
    17341 aagctcggtg aagtatggct atctttacac aacaaaaaat gagtaagatg ccagctggca
    17401 aattgtacat gatcccatct gcactttcag aacattcggt ctatacaccc ggtaggcatg
    17461 agcttgaagt tatacattcg ctcaggcttt ttctggcaga acgggcaaag acagcgcgac
    17521 attatattgc tactacacta ccaccctaca aacaacagga aatcgacgta ctcgaactac
    17581 caaagcacgg agatgatggc actgtgaaag atttcatcaa aagaatacaa tctggtgaaa
    17641 gcggtggctt tctgtcagaa gcaggatgtc cgggcatagc tgacccgggt gctgaagtag
    17701 tatcacaagc atatagtgca ggcatagaag ttgtaccgct ggtaggccca tcttctattt
    17761 tactagcatt aatggcttcc ggtatgagtg gccaacattt ctgttttaag ggatatttac
    17821 cgcaggacaa aacaaaactt gctcaggtgc tggtatctat ggaggcagag tcggcacgta
    17881 ctgattgtac acagatatgg atagagacgc cttaccgcaa caatgccatg cgtgaagttt
    17941 gtctgaaaac actgaaggct ggcaccagat tctgtatcgc agcaaacctg acaggaccac
    18001 aagagaggat attttcagca aaaattaaaa actggcaacc tacaaagctt ggagaccttc
    18061 aaaaaacacc ggcaatattt ttgatgt
//
LOCUS       DAOQQY010000023        17670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_986_length_17670_cov_5.602895, whole genome shotgun sequence.
ACCESSION   DAOQQY010000023 DAOQQY010000000
VERSION     DAOQQY010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17670
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_986_length_17670_cov_5.602895"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PLE23_02485"
     CDS             <1..946
                     /locus_tag="PLE23_02485"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HMX82163.1"
                     /translation="QKTIDKLNSYNVLSVLDYGAEGNESEHSFDATMNEFLRAAEFAS
                     LHDSVPVISMKITGLARFGLLEKISSGNPLNESETKEYESLLKRVDAVCHKAHSKNVA
                     VFIDAEESWIQQAIDDLVDKMMERYNQTRVIVFNTFQMYRHDRLAFIHKSYERALAGN
                     YFFGAKLVRGAYMDKERKRAQEMGYPDPIQPDKNATDRDYDAGIRFCLEHYEKISVCN
                     ATHNLESCKKMADFIEGMNVSKNHPHLNFCQLYGMSDNLTFNLAAAGYNAGKYLPYGP
                     VKDVIPYLVRRAEENSSITGDIGREYSLVLSEMKRREL"
     gene            complement(998..3895)
                     /locus_tag="PLE23_02490"
     CDS             complement(998..3895)
                     /locus_tag="PLE23_02490"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HMX82164.1"
                     /translation="MKKSWLFLFLITFNWGKIYSQPVPTQAAERDKAFAQRILLDDIS
                     LLKPVKFKSIGPTVMSGRVVDIDVNPSNYNEFYVAYASGGLWKTSNNGQSFTPLFDKQ
                     AVMTIGDIAVNWKTGRIWIGTGENNSSRSSYSGNGMYISDDGGKTWTNKGLWDSHHIG
                     RIILHPSDNNIAWVAAAGHLYSDNSERGVFKTTDGGQTWNKTLYLDEKTGAIDLLMNP
                     ANNQQLWAAMWQRDRKAWHFTESGSGSGIYMSNDGGNTWQLITSAGSGFPSGPGCGRI
                     GLSISRKNNQDMIYAVIDNQAKRPTDKTEIKKAELVKSDFRQMTKEQFLAIPPKKLKA
                     FLVKNNFPVKYSAEKVIKMIKSDKIQPSALADYLEDANAALFSSDQPIEGAEVYSSAD
                     NGKTWQKMNKENLDGLFYSYGYYFAQIRTAYDNPDQVFLLGVPLLMSNDGCKTWEAID
                     GDNVHADHHALWINPANNGHLINGNDGGINITYDYGKTWAKCNSPAVGQFYSVNYDMA
                     KPFNIYGGMQDNGVWKGSSENELSDRWHSTGEYGFKSYIGGDGMQVAIDNRDNQTVYT
                     GYQFGNYFRINQKNGDYEFISPTHELGERPLRFNWQSPVALSTFNQDILYFGANKLYR
                     SMNQGKTWTAISDDLTNGGKPGNVPYGTLTTFHESPLKFGQIVTGSDDGKINLTRDGG
                     YTWIDISKGLPAILWVSRVQFSAFSLSRIYISLNGYRNDYFESHVYMSDDYGNNWTRI
                     GTTLPAEPINVIKEDPQNEDILYVGTDHGLYVSLDRGKSFMGMNHSLPGVSIHDLFVH
                     PRDHKLVIGTHGRSIYITDVANVQQLNNENLAKELMVFPINTIQADAWGKKFSAWDAP
                     KSPEIEISFYSKEPGLSNISITDEDKNVLFSTSINAIKGLNKFVYNGTVDEKIIPKYT
                     NTLLKAARKENPEAEIMLNKADDGKYYIQSGKYTATIKRNNHIAETEMEIE"
     gene            4024..5553
                     /locus_tag="PLE23_02495"
     CDS             4024..5553
                     /locus_tag="PLE23_02495"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMX82165.1"
                     /translation="MFKKLDKLLVTSFIPPFIVAFCIAVFVLMMQFVWVYIDEIIGKG
                     VELFVLIELLGYLSVSRIPMALPLGVLIASVMVMGNLAERYELSSFKSAGISLYRVML
                     PLMYVMAAVSLFSFYCSNNLIPVANLKFGSRLYDIRRQKPALSLEEGVFNDDFEDFVI
                     RIKNKLADNQTIEDVLIYNQTGNFDQINQITAKSGKMFTSADKQYLVMQLKDGVQYQE
                     LNPSNQKNEKYPFVRTSFQKLQKVYDLREFSMNKTDEELFKSHQMMLKTAELSHKIDS
                     INQKVAERRHSVAEMVPRYLTALKEKPKTDGKAKSKADSLMKELREQPIEIRYEDPAA
                     QTKKPEQVQSFIQLYTQDKQESLLARAKTTTRSVQSELISLNESIQRSLKARNQHQYE
                     MHIKYSIAFICFVFLFIGAPMGAIVRKGGFGYPLLIAIVYFMIFIVLNILFKNLTEQL
                     VLNGVLAAWMPVLVLAPLGMFLTMKAARDTSFFNTDQFAARMLKVWHWVMRKKVSSHT
                     S"
     gene            5555..6202
                     /locus_tag="PLE23_02500"
     CDS             5555..6202
                     /locus_tag="PLE23_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMX82166.1"
                     /translation="MLFRLFRYRHIYIPLLLLWVVGLILISNTFKGQEVLYVETHRNH
                     FWNAIFGSVTQLAEFPVYAIIVVLLWLSNRREKALTVMIAGLITIFVSGGFKEIFQEP
                     RPAKYFETTNPAIELPAVPGIALHRGRNSYPSGHSMGAFTLFVTLGYLWPRQRIFGVL
                     CVMTAATVAFSRIYLGQHFLTDIVAGSLVGTYIASFSYFVIFKKILKIKTKKHLT"
     gene            6267..10700
                     /locus_tag="PLE23_02505"
     CDS             6267..10700
                     /locus_tag="PLE23_02505"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMX82167.1"
                     /translation="MRKIFLLFLFISGSLSLFAQPVYDDCPAYNVGVAPFCNPSVFFT
                     NVDATASDIGAKNIPNCFSDGDVNRDVWISFVASDTILDYVITVTGLKDGTTQAMKYP
                     EIALYRGDCGVDELALVLCAGYNPAVSDTSVTLTATGLTPGATYFMRIHDNKKGDNTV
                     HSGTFVLCIKEKDPVISITEGFSNACSGKIVDSGGETGNYKSNENYFFKICPSGKPSC
                     IVLTLDYYNIDNAGDALTFYDGDNTASPVIDVLSSNNQTGGGAVAYKVKATSGCLSIG
                     FKSDASLNLDGFSGSWQCSIAPCPPDEVIIVIPDVTKEQIIASVSTPATVVTIDTIKC
                     ASGAYGSFTATDNSNLGLKKGLLLTTGTVAGAQGPNNSGSTSGFANTPGDSDLDYLSQ
                     LQGSQTLSNDACIVELDVFAATDELAFEYVFGSEEYPEFVNSNFNDIFAFFISGPGIV
                     GDPNIKNQKNIAVLPDNTTFVQINSVNNLLNWEYYRNNLGGQTIQYDGLTSDFKGVKT
                     SLSAKSKVTPCNTYHLKFAIADRGDSSWDSGVFISDLKSGTVNFNVTYNSGIDYMIEK
                     CTGEDVVNIKLSNPLDYSVSYKVAVGGTATKGVDYNMTIPNTITFNAGETVLSFPINP
                     IADAITEGVETITITLSNNFGCGDVTYAVLTIELHDAPYAAILNDVDTAFYCKGEKIN
                     LSVSGCNTYTWSPAGIMNDPNIANPVASPTDDGYIYVTGEIAGCVAYDSIYLKELNPQ
                     VKILPLATDKLCEGKSVKLTATNNTFNQGFSWTPAIGLNSTTNSTVTASPKVTTTYYA
                     QVDLSGCVVKDSVTLLVDPFDFPTVFSDTFKLCQSYPIKLASDIFFSTTTYQWTPAKY
                     LDNPNASGPIATPGEDLLYTLVATSEKGYCVDSAKVYIDVIDAEVNILDQAGIEKDTF
                     YLCKGDSLQLNSTIKPLGSQIKWSSEEGTINNDQAGSVVIKPTYSQWFVSKLTTSECV
                     VYDSVYVQVDSLPLDLGIHPADTTVCQGSIVVLKGNTYEQGHFPNLIFNWEPTFDAKT
                     PDSLYNIVIEANASYTYQRFAYSGACVNISEAKVNVIPTNFLSISPPQATVCPGTTIE
                     LKAEAAQPGVTNFEWSPADGLSCTQCQTTIATITGGSKTYQVKGEFMGCPVQASVTIN
                     ESPLPWVNFPADNIICESESTPLNESPDPTFTYKWTSTDPNFGTSVSSNPVVSPLKTS
                     TYYVTVSNNIGCTAIYQHEIVVAPTPTLTVSADVTICPGEEVTLTATSNAGGNYTWSD
                     GQTGSTIVVKPGGNTIYTVSFEDTYGCGKATASTIVSVDVVPLVSIKATPDSLNVNQG
                     DPVTLEAVTDPPGAIVTYAWKENGNALSQTTKVITVTPLENPTKYTVTVTTPKGCAVS
                     SEIEFNVLPAEWDVPNVFTPNGDGKNDVFKIVLKGKNISISQFSVYNRWGQKIYDKAD
                     NNGWNGDVNGKPAPTDTYVYWIVLKTPKGDEVLKGDISLIR"
     gene            10871..12676
                     /locus_tag="PLE23_02510"
     CDS             10871..12676
                     /locus_tag="PLE23_02510"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMX82168.1"
                     /translation="MKALDTLSLPEITILEKQHILHALGRNISETDTVFKIFSDHSLN
                     ALIESAGIGILKNYGAGNSSIFSNQGTAAQHNQVVWNGIACNNALLGSSDISLLTSGE
                     LNDIKIIQGTTSSYWGGGAIGSTLLLRTLFDDRYLISVGMAYQSLGNQRLSVSISKNL
                     GKYAVYSKISAGRGENKFTVLNYTDPKKTERTFSAPYDERQVEFSTQRSFNKSRIQAH
                     IMYADMARQLSNSLTEAETDARQGDRISRGVLQYDWSLAKMRITSLAGYTFDRIIYKA
                     APVHDTGLVHAVFLKSEIKYNFKFGQLYAILQNKYEAASNSSFKNKVSRNTASVIAGF
                     TTRLAKNLSSNVEVRVEKQGGNKTIAVASASIESSYRGIDLKTYVSGSYNNPTLNDLN
                     WVPGGNIHLKPEKGINAGVTISKKWYISPIENKTTLEGFYHNIQDWIQWVPQGSIWSP
                     VNFKHVISSGVVFDQQSLLQTGSSHFILNIKGTYTHAVNISKLPEANYNKQLAYIPGL
                     TGMFSLSYISGSKLSLRAELLSVGERYTVFDQSASLPSYAIVNLYAGKKIVLKKTELL
                     PYLQVNNLLNKYYESVAFRPREPLHFLIGFKINIL"
     gene            12673..13701
                     /locus_tag="PLE23_02515"
     CDS             12673..13701
                     /locus_tag="PLE23_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82169.1"
                     /translation="MKKILIVCLALSAFFSCKKDDPITPGKYGAGLLILNEGPFGTGS
                     GTIGFWDRSDKAPVNDVFETENTGAKLGNILQSMTQSGEKFYFVVNNSNKVVVTDKNF
                     KYLSEISGLQLPRYLVVKGSKAYISQWGSDGLTGSLVVADLATGQLTKTIPLGKGPEN
                     MLIKGDKLYVSMVGGYDRDNRVMVVDLVGETVSKTITVDDNPSNIIDYNGQILVLCKG
                     FTDYVNPANSTNGKIVEISNDAVSATYNLSEQADNLIRKGNSSTLYFTLNGKPSKWEY
                     KAANFETLQGAPFIYKLSYDDVAGKVLMSDAVDFASKGKILVADENLNVSKTIDAGII
                     PSLVYVIK"
     gene            complement(13702..13902)
                     /gene="xseB"
                     /locus_tag="PLE23_02520"
     CDS             complement(13702..13902)
                     /gene="xseB"
                     /locus_tag="PLE23_02520"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HMX82170.1"
                     /translation="MKEKNLTYDQAYNELQKILSDLQNSETGVEELTEKIKRASELVT
                     YCKNRLRSTEKEIQKILNEPEN"
     gene            complement(13902..15137)
                     /gene="xseA"
                     /locus_tag="PLE23_02525"
     CDS             complement(13902..15137)
                     /gene="xseA"
                     /locus_tag="PLE23_02525"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMX82171.1"
                     /translation="MNQSLRLYQLNEYIRRVIALNFSESIWINCEIASFRESRGHAYL
                     ELIENSEFDHEIIAQASAVIWRTQLMAISKKLGPEKESVLQPGKEILIKVKVEYNEKY
                     GLKLIVEDIDLSFTLGKLALRRQEILNELAKQNLLGKNAEIALPYVMQRIAVISSENA
                     AGWQDFKNQLQDNIFGYHHEVTLFHSAMQGQNVEPEVTENLKEIIKQKDKFDCVVLIR
                     GGGGKVDLSDFDNLLIAKTIAGMPLPVFTGIGHDIDQSIADMVAHTSLKTPTAVAEYI
                     IQHNMRYESNIIELGRSISIQIQNNLQQHTEVLDYAKQRLNWIATSAISRAESSLDLI
                     FGQIPMVATHKLNINLTQLDHISTLLSQLSPEAHFKRGYSIVEHNNARIGKKNKPKVG
                     DEINIYSIIGKMTGNITKI"
     gene            15274..15753
                     /locus_tag="PLE23_02530"
     CDS             15274..15753
                     /locus_tag="PLE23_02530"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HMX82172.1"
                     /translation="MNEENFLKTIKTPWKFRWYLFTKLPLAWLMGIRLLVIDHSHCTV
                     ILPFKKRNLNPFRSMYFAAQVAAAELSTGILLVMAREAAGDVSMLVSRIESEFYKKAV
                     TDLRFTCNQGEMITDIIRKAVVSGDPMTIVAESIGKDEHEDTVSITRITWSVKRRKK"
     gene            complement(15759..16313)
                     /gene="def"
                     /locus_tag="PLE23_02535"
     CDS             complement(15759..16313)
                     /gene="def"
                     /locus_tag="PLE23_02535"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMX82173.1"
                     /translation="MILPIYAYGQPVLKKVATDIDSSYPNLTTLLENMWQTMYNAHGV
                     GLAAPQIGLDIRLFIVDTIQTMDEDKASDGIKTVFINARILQESGEEWAYEEGCLSIP
                     FVRGDVMRQPNITIEYYDENFTKHTKSFDGLNARVIQHEYDHIEGVLFVEKLKPLKKK
                     LIQKKLENIKKGNIQAEYKIKFVN"
     gene            complement(16374..16805)
                     /gene="ruvX"
                     /locus_tag="PLE23_02540"
     CDS             complement(16374..16805)
                     /gene="ruvX"
                     /locus_tag="PLE23_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HMX82174.1"
                     /translation="MSRIIALDYGSKRTGIAVTDPLQIVPNGLETLQTKDLMTFLEKY
                     FETEQVSMLVIGYPTHLDGNDTYLTNEIDVFLNNFAQKFPQIKIFRQAEWFTSRQASQ
                     VILQSGIGRQKRQDKSLIDKVSAVIILEEFLHSKAYQDSRI"
     gene            complement(16851..17234)
                     /locus_tag="PLE23_02545"
     CDS             complement(16851..17234)
                     /locus_tag="PLE23_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82175.1"
                     /translation="MKKTVYLFVFALLSFTIASCSKSAPDQPDLTAQVEGTYQGQLIT
                     GTSNYVTTQVKLTKIDNITVKMEHLNGNPAFAQDNISLSESGTSDIQGDSQSWLIKWS
                     KISPDKQSLAVKNKLDNTVFSGEKQ"
     gene            complement(17427..>17670)
                     /locus_tag="PLE23_02550"
     CDS             complement(17427..>17670)
                     /locus_tag="PLE23_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82176.1"
                     /translation="NNPNPFGDVTNISFYLPQDEYAQLVITNVEGKVVKMIEGDFNKG
                     LNQIKLHKAEIGAGGIYYYQLNTATESKTMKMIIIE"
BASE COUNT         4996 a         3400 c         3815 g         5459 t
ORIGIN      
        1 ccagaaaacc attgataaac ttaactccta caatgtgcta agtgtactcg attacggagc
       61 agagggcaac gaatcagaac actcatttga tgctaccatg aacgaattct tgcgcgccgc
      121 tgaatttgct tctctgcatg attctgtacc ggtaatcagt atgaaaataa ccgggcttgc
      181 aaggttcggc cttcttgaaa aaataagcag tggcaaccca ctaaatgagt cagaaaccaa
      241 agaatacgaa agccttctga aacgtgtaga tgctgtttgt cataaagcgc actctaagaa
      301 tgttgcagta tttattgatg ccgaagaaag ctggatacaa caagctatcg acgacttggt
      361 ggataaaatg atggaacgat ataatcagac aagggtcatt gtcttcaata catttcagat
      421 gtacaggcat gacagactag cctttataca caaatcatac gaaagggcat tggctggaaa
      481 ttattttttt ggtgcaaagc tggtacgtgg tgcatatatg gacaaggaga gaaaacgagc
      541 tcaggaaatg ggctatcctg acccgattca accagataaa aatgcaaccg accgcgatta
      601 cgatgcaggt atcagattct gccttgagca ttacgaaaaa atttctgtct gtaatgcaac
      661 gcataacctc gaaagctgca aaaagatggc cgattttata gaaggaatga atgtgtctaa
      721 aaatcatcct caccttaatt tctgccaact ctatggcatg agtgataatc ttacatttaa
      781 cctggctgct gccggataca atgccggaaa gtatctgcct tatggtccgg ttaaagatgt
      841 tattccgtat ctggtacggc gggctgaaga aaattcgagc ataaccggtg atattggtag
      901 ggagtatagc cttgttttat ctgaaatgaa aagacgcgaa ttgtaacttt ttatgtagtt
      961 caatgacatt ccgcatcttt tcggattgta tataggttta ttctatctcc atttcagttt
     1021 cggcaatatg gttattgcgt ttgatagtgg ctgtatattt ccctgattgg atataatatt
     1081 tgccgtcatc tgctttgtta agcattattt ctgcttcagg gttttctttt ctggctgcct
     1141 tcaggagtgt gttggtatat ttcgggatga ttttttcatc taccgtgcca ttatatacaa
     1201 atttgttgag accttttatg gcattgattg aagtgctgaa taggacattt ttgtcttcat
     1261 cggtaatgct tatattggat agtcctggtt ctttgctgta aaatgaaatt tctatttcag
     1321 gtgattttgg agcatcccat gcagaaaatt tcttgcccca tgcgtcggct tgtattgtat
     1381 taatagggaa taccattaat tcttttgcca gattttcatt atttaattgc tgcacattgg
     1441 caacatctgt tatgtaaata cttctgccat gggtacctat taccagctta tggtcacgcg
     1501 gatgtacaaa caggtcatga atggatacac ccggcagtga gtggttcatg cccataaagc
     1561 tttttcctcg gtcaaggctc acatacagac cgtggtcagt gcctacatat agtatatctt
     1621 cattctgagg gtcttccttg atgacgttga tcggttcggc aggtagagtt gtgccgatgc
     1681 gtgtccagtt attgccatag tcatcagaca tatagacgtg actttcgaaa taatcattgc
     1741 ggtagccatt caaactaata taaatccttg aaagagaaaa tgccgagaac tgaactcttg
     1801 aaacccatag tatggctggt aatcctttgc ttatatctat ccaggtgtag ccaccatctc
     1861 tggttaaatt tattttacca tcatcgcttc cggtgactat ctgcccgaat tttaatggac
     1921 tttcgtgaaa agttgttaaa gtaccataag gcacattgcc gggtttgcca ccgttggtta
     1981 agtcgtctga tatggctgtc catgttttgc cttggttcat ggagcggtaa agtttattgg
     2041 ccccgaagta aagtatgtcc tgattaaaag tagaaagcgc taccggactt tgccagttga
     2101 agcgcaatgg tcgttcgccc aattcgtgtg taggtgatat gaactcataa tctccgtttt
     2161 tttgatttat tctgaaataa ttgccaaact ggtatcctgt atagacggtc tggttgtcgc
     2221 ggttgtcaat ggctacctgc ataccatcgc caccgatata acttttaaat ccatattctc
     2281 ctgtactgtg ccatctgtcg gacaattcat tttcagaact gcctttccac acaccattgt
     2341 cttgcatgcc accatatatg ttgaagggct ttgccatatc ataattaacc gaataaaact
     2401 gcccaaccgc cggcgaattg cattttgccc aggtttttcc ataatcgtag gtaatgttga
     2461 tgccaccgtc attgccgttg atgagatgac cgttgttcgc agggttaatc cataaagcgt
     2521 ggtgatctgc atgtacattg tctccatcaa tggcttccca tgttttacag ccatcatttg
     2581 acatgagcag aggcacaccc aataggaaaa cttggtcagg attgtcgtag gcagtccgga
     2641 tctgtgcaaa ataatacccg taggagtaaa acaagccatc gagattttct ttattcattt
     2701 tttgccaagt cttgccgtta tctgctgagc tatatacttc agcgccttct atcggttggt
     2761 cagatgaaaa caaagcggca tttgcatctt caagataatc tgcaagtgca gagggttgta
     2821 ttttgtccga ttttatcatt tttatgactt tttcggctga atattttaca ggaaaattat
     2881 tttttaccag aaatgctttg agtttttttg gaggaatagc gagaaactgc tcctttgtca
     2941 tctgacgaaa atcggacttt acaagttcag ctttttttat ttcagttttg tctgtcgggc
     3001 gtttggcttg gttgtctata acagcataaa tcatatcctg attgtttttt cgtgaaatag
     3061 atagaccaat gcgtccgcag cctggtcctg aaggaaaacc tgaaccggcc gaagtaatca
     3121 attgccaagt gttgccgcca tcattgctca tgtaaatgcc cgagcctgag ccgctctctg
     3181 taaagtgcca ggcctttctg tcgcgttgcc acatagctgc ccacagctgt tgattgttgg
     3241 caggattcat taacaaatcg atggctccag tcttttcatc gaggtaaagt gttttattcc
     3301 aggtctgccc gccatctgtg gttttgaaga ctccacgttc actattatca gaataaaggt
     3361 gccctgcagc agccacccat gctatattgt tatcacttgg gtgtaggatg atacgcccga
     3421 tgtggtggct atcccacaat cctttatttg tccaggtttt tccgccatca tcggatatat
     3481 acataccatt gccactgtat gatgatcgtg aagaattatt ttctcctgta cctatccaga
     3541 tgcgtccggt tttccagttg actgctatgt cgcctatggt catcactgcc tgcttgtcga
     3601 ataaaggagt aaaactctgt ccgttattcg atgttttcca aaggccgcca gatgcgtatg
     3661 caacataaaa ttcgttgtaa ttgctcgggt tgacatcaat atccactact ctgccactca
     3721 taacagttgg gcctatagat ttaaatttga ccggctttag caatgatatg tcatccagca
     3781 gaattctttg tgcaaatgct ttgtcacgtt cggcagcttg tgttggaacc ggttgagaat
     3841 atatttttcc ccagttgaat gtaatcaaga ataagaatag ccagcttttt ttcattggtt
     3901 gtaaaattta ggaaaggtaa atatagcatt taattcagtt tgtattcatt ttttaaaggt
     3961 acgatagcac aaacagtgga ataaaattca cttcatttcg ttgatagttt gtaaatttgt
     4021 tgaatgttta aaaagctcga caaactcctt gttaccagtt ttattcctcc attcatcgta
     4081 gcgttttgta ttgctgtttt tgtgttgatg atgcagtttg tatgggtgta tattgatgaa
     4141 attatcggta agggagtaga attgtttgta cttatagagt tgctgggtta tctatctgtg
     4201 tcaagaatac caatggcgct accactcggt gtactgatag cttcggtaat ggtcatgggc
     4261 aacctggccg aacgatatga gctttcgagt tttaagtcgg ccggtatttc actatacaga
     4321 gtgatgttgc ccttgatgta tgtgatggct gcagtttctc tcttttcatt ttattgctca
     4381 aataatctta taccggtagc caatcttaag ttcggaagcc gattgtacga tatcaggaga
     4441 cagaagcctg cacttagtct tgaagaaggt gtcttcaatg atgattttga ggatttcgtt
     4501 atccgtatca agaacaaatt ggccgataat cagaccattg aagatgtatt gatatataat
     4561 cagaccggca attttgacca aatcaaccag attacagcca aaagtggtaa aatgtttacc
     4621 agtgcagata agcagtacct tgttatgcag ttgaaggatg gtgtacaata tcaggaactc
     4681 aatccgagta atcagaaaaa tgaaaaatat ccttttgtac gtacgagttt tcaaaaactg
     4741 caaaaagtgt atgacctgcg ggagttttcg atgaataaaa ccgatgaaga gctttttaag
     4801 tcgcaccaga tgatgctgaa gacggcagaa ctgtcacata aaatagacag tataaaccaa
     4861 aaggttgcag aacgccgtca ttcggtagca gagatggtac ccagatacct tacagccctg
     4921 aaagaaaagc caaagacaga cggaaaagcc aaaagcaaag ctgacagcct catgaaagaa
     4981 cttcgcgaac agcctataga gataaggtat gaagacccgg cagcgcagac caaaaaacct
     5041 gaacaggtac aaagtttcat ccaattatat actcaggata aacaagagag tctgcttgca
     5101 agggcaaaga ctacgacccg tagcgtacag agcgaactca tctcattgaa tgaaagtatt
     5161 cagcgaagcc taaaagctcg taatcaacat cagtatgaaa tgcatattaa atatagtatt
     5221 gcttttattt gctttgtatt tttgtttatt ggcgcaccta tgggtgcaat tgtgagaaag
     5281 ggcggtttcg gctacccatt acttattgct attgtatatt tcatgatatt tatcgttttg
     5341 aatattttat ttaaaaatct gacagaacaa ctcgtcctta atggtgtgct tgctgcctgg
     5401 atgccggtat tggtacttgc cccgcttgga atgtttctta caatgaaggc cgcacgtgac
     5461 acatcatttt tcaacacaga tcagtttgca gccagaatgc tcaaggtatg gcattgggtc
     5521 atgaggaaaa aagtaagcag ccacacctct taatatgtta ttcagattat ttcgttacag
     5581 acatatttat attccattac tgttgctatg ggtagtaggt ttaatactta tatccaatac
     5641 ctttaaagga caagaagtgc tgtatgttga aacgcaccga aatcatttct ggaatgctat
     5701 ctttggctct gttacccagc tggctgagtt tccggtatat gcgatcatcg ttgtgttgtt
     5761 gtggctgtct aatcgacgcg aaaaggcact taccgtaatg atagcagggc tcataaccat
     5821 ttttgtaagt ggtgggttta aggaaatttt ccaagaacca agaccggcaa aatattttga
     5881 aacaaccaac ccagccatcg aacttcctgc tgttccgggg atagcgttac accgggggcg
     5941 aaacagttat ccgagtggtc actctatggg ggcatttaca ctttttgtaa cgctgggtta
     6001 tctttggcca agacagcgaa tatttggcgt gttgtgtgtc atgacggccg ctaccgtggc
     6061 attttcgagg atatatctcg gtcagcactt ccttacagat atagttgcag gctcattggt
     6121 aggtacttat attgcttcat ttagctattt tgtaattttc aaaaaaatac taaaaatcaa
     6181 aacaaaaaag catttaacgt aaccaatatt gataatttgt ttaattttgg cagttaagaa
     6241 atgtttatac acaaacaaat acagttatga ggaaaatttt tctccttttt ttattcatat
     6301 ccggaagcct tagtttattt gctcagccgg tatatgatga ttgtcctgct tataacgtag
     6361 gcgttgcacc tttttgtaat ccttcagtat tctttaccaa tgttgatgct accgccagcg
     6421 acataggtgc caaaaatatc ccaaactgct tcagtgacgg tgatgtaaat cgcgatgtat
     6481 ggatatcttt tgttgccagt gataccatac tggattatgt gataaccgta acaggcctga
     6541 aagatggtac tactcaggca atgaaatatc ctgagattgc actgtacaga ggtgattgtg
     6601 gtgttgacga gcttgcatta gtactttgtg caggatataa cccagctgta agtgatacgt
     6661 cggtgacctt gactgctacc ggtcttacgc cgggtgctac ttatttcatg agaattcatg
     6721 ataacaaaaa aggagacaat acagtccatt caggtacatt tgtactttgt attaaagaaa
     6781 aagatccggt catttctata actgaaggct tctccaatgc ctgctccgga aaaatagtag
     6841 attcgggtgg agaaaccggt aactataaat caaacgaaaa ttatttcttc aaaatctgcc
     6901 cttccggaaa accatcctgt atcgtactga cacttgacta ttataatata gataatgcag
     6961 gagatgcact cactttttat gatggcgata ataccgcaag cccggtgatt gatgtacttt
     7021 caagcaacaa tcagacaggt ggtggcgcag tggcctataa agtaaaggct acctcaggtt
     7081 gtctgtctat tggatttaaa tctgatgcat cgcttaatct tgatggattt tcgggttcgt
     7141 ggcagtgtag tatagctcct tgtccgccgg atgaggtcat tattgtaatt ccggatgtta
     7201 caaaagaaca gattattgct tcagtatcga ctccggctac ggtcgtaaca attgatacca
     7261 tcaaatgtgc aagtggggcg tatggttcgt ttactgctac tgataattca aatcttgggt
     7321 taaagaaggg attgttactt accaccggta cagtagccgg tgcgcaaggg cccaataata
     7381 gcggttctac ttcaggtttt gccaatactc cgggtgattc cgatcttgat tatttatcgc
     7441 agttacaagg tagtcaaaca ttgtcaaatg atgcatgtat cgtcgagttg gatgtatttg
     7501 cagctaccga cgaacttgcc tttgagtatg tattcggttc ggaggaatat cccgaattcg
     7561 ttaattccaa tttcaatgat atcttcgcat tctttatttc cggaccggga attgtcggtg
     7621 acccaaatat aaagaaccaa aaaaatattg cagtactacc ggataatacc acttttgtac
     7681 aaatcaattc ggtgaataat ttgcttaact gggaatacta tcgcaacaat ctcggtggac
     7741 aaacaataca gtacgatggg cttacctctg atttcaaggg cgtcaaaacg agcctttcgg
     7801 caaaatcgaa agttacacct tgtaatacct atcaccttaa gtttgcaata gctgaccgtg
     7861 gtgacagctc atgggactcc ggcgtattta tttctgacct aaagagtggt acggttaatt
     7921 ttaatgtaac ctacaattct ggtatagatt atatgataga aaaatgtacc ggtgaagatg
     7981 ttgtcaatat taaattatcg aacccgctcg attattctgt atcgtataaa gtagcagtag
     8041 gcggtactgc cacaaagggt gtggattaca atatgaccat acctaatact attacgttca
     8101 atgccggcga aacagtttta agttttccga tcaatccgat tgctgacgca attaccgaag
     8161 gtgtcgaaac aataaccatc acactaagta ataacttcgg atgtggtgat gtaacctacg
     8221 ccgtattaac catcgaattg catgatgcac cttacgcggc tattttgaat gatgtggata
     8281 ctgcttttta ttgtaaaggt gaaaaaatca acctctctgt gtcaggctgt aatacttata
     8341 cctggtcacc tgcaggcatc atgaatgacc caaacattgc taatccggta gcatcgccta
     8401 ctgatgatgg ttatatatat gtaaccggtg agattgcagg ttgtgtcgct tatgattcaa
     8461 tttatttgaa ggaactaaac cctcaagtga agattctccc gcttgccaca gataaactct
     8521 gcgaaggaaa gagtgttaaa ctcactgcca ccaataatac tttcaaccag gggttctcct
     8581 ggacacctgc tatcggatta aacagtacta ccaattcgac tgtaacagca tcaccaaaag
     8641 tgacgacaac atattatgcc caggttgatc tatcgggttg tgttgtgaaa gattcggtaa
     8701 cattgttagt tgatccattt gattttccta cagttttttc tgatactttc aagctttgcc
     8761 agtcatatcc tatcaagctg gcttcggata ttttcttctc tacaacaacc taccagtgga
     8821 cacctgcaaa atatctcgac aatccgaatg cttcaggccc aatagctaca ccgggcgaag
     8881 atttactata tacattggta gctacaagcg agaaaggcta ttgtgttgat tcggctaaag
     8941 tgtatataga tgttatagat gcagaagtga atatacttga tcaggctgga atagaaaaag
     9001 atacatttta cctgtgtaaa ggtgacagcc tacagctcaa ttcaactatt aaaccactag
     9061 gatcgcagat aaaatggagc tcagaagaag gcaccatcaa caatgaccag gctggaagtg
     9121 tagttatcaa gcccacttat tcgcaatggt ttgtttcaaa actcactaca tctgaatgtg
     9181 tcgtttatga ttccgtctat gtacaagttg attcattgcc actagacctc ggtattcatc
     9241 cggcagatac tacggtttgt caggggtcga ttgtagtatt gaaaggcaat acttacgagc
     9301 aagggcattt ccctaatttg atattcaact gggaaccaac atttgacgcc aaaacacctg
     9361 attcgttata caacatagtt atagaagcaa atgccagtta cacttatcag cgatttgcat
     9421 acagcggggc ttgtgtgaat atatcagaag caaaggtgaa tgtgataccg actaacttcc
     9481 tgagtatttc tccaccgcag gctacggtct gtccgggtac aaccattgaa ctaaaagccg
     9541 aagctgcaca gcccggtgtt accaacttcg agtggtcacc tgctgacgga ctttcgtgta
     9601 cacaatgtca gactactatt gccaccatca caggtggctc taagacatat caggtaaagg
     9661 gcgaatttat gggttgtccg gtgcaagcaa gtgtaaccat taatgagtca ccactgccat
     9721 gggttaattt cccggcagac aatattatct gtgagtcaga atcaacacca ttaaatgagt
     9781 caccagatcc gacattcaca tataaatgga caagtaccga tcctaatttt ggtacttcag
     9841 tgagttcgaa tcctgtggtt tcgccgctca agacttctac ttattatgtt actgttagca
     9901 acaacattgg ttgtacagct atctatcagc acgaaatcgt agttgcccca acgcctaccc
     9961 ttacagtttc agcagatgtg accatctgcc ctggcgaaga agtgacgctt accgctacat
    10021 cgaatgcagg cggtaactat acttggagcg acggccagac cggttctacc attgttgtaa
    10081 aacccggagg aaatactata tataccgtgt catttgaaga tacctacgga tgtggaaaag
    10141 ctacagcatc taccatcgta tcggttgatg tggtgccttt ggtatcaatc aaagcaacac
    10201 ctgattcact gaatgttaat cagggcgatc cggttacgct tgaagcggtg actgacccac
    10261 ctggcgctat tgttacgtat gcctggaaag aaaatggcaa tgctcttagt cagactacta
    10321 aggtgattac tgttacacct cttgagaatc cgaccaaata tactgtcact gttactacac
    10381 ctaagggttg tgcggtatca tccgagattg agtttaatgt actccctgcc gaatgggacg
    10441 tgccaaatgt ctttactcct aacggggatg gcaaaaatga cgtgtttaaa atcgttctga
    10501 aaggcaagaa tataagcatc agccagttta gtgtttacaa taggtgggga cagaagatct
    10561 acgacaaggc cgacaacaat ggatggaacg gtgatgtaaa cggtaagccg gcacctactg
    10621 atacttatgt ttactggata gtgttaaaaa cacctaaagg tgacgaggtt ttgaaaggcg
    10681 atatttcttt gataagataa gataatatac ttacaatata acagaggagg tcagcgtatg
    10741 ccggcctctt tttttttgtc atattaaagt taaattgtat ctatgagggt aaatctcagg
    10801 tatatttctt tagttttacc gcatgaaatt ggtggcgatt tttttgatgg taatttgtgt
    10861 aaatgaaagc gtgaaagctt tagatacgct cagccttcct gaaattacca tccttgaaaa
    10921 acaacatatt ttacatgcac ttggtaggaa tatttctgaa acagatacag ttttcaaaat
    10981 attttctgac cacagcctca atgcactgat agaatcagcc ggaattggta tattgaaaaa
    11041 ttatggtgcg ggtaatagtt ccattttctc aaatcaggga acggctgcac aacataacca
    11101 agtagtatgg aacggtatag cctgtaataa tgcactgctg gggtcgagcg atatttcttt
    11161 gctcacatcg ggtgaattga atgacatcaa aatcattcaa ggaacaacca gcagttattg
    11221 gggaggtggt gctattggtt ctaccttgct gctaaggact ttatttgacg acagatatct
    11281 catttctgtc ggtatggcct accagtcact cggtaatcaa agactgagtg tcagtattag
    11341 taagaatctt ggcaagtatg ctgtatattc gaaaatatca gccggtagag gagagaataa
    11401 attcactgtg ctgaattata ctgatccgaa aaagactgaa agaacatttt cggctcccta
    11461 tgatgaaaga caagtagagt tttcaactca acgaagtttc aacaaaagtc ggatacaggc
    11521 tcatatcatg tatgccgaca tggcaagaca attaagcaat agccttacag aagctgagac
    11581 agatgccagg caaggagaca ggatatcccg aggcgtgttg cagtacgatt ggtctttagc
    11641 caaaatgcgg ataacttcgt tagcaggata cacttttgac agaataatct ataaggcagc
    11701 tccggtacat gataccggac tggtgcatgc tgtattttta aaatcggaaa ttaagtataa
    11761 cttcaaattt ggtcagttat atgccatttt acaaaacaaa tatgaagcag cttctaattc
    11821 ttcatttaag aacaaggttt cgagaaatac tgccagtgtc atcgcaggct ttactacacg
    11881 tttggcaaag aatctcagct caaatgttga agtccgggta gaaaagcaag gaggcaacaa
    11941 aactattgcg gtagcttctg caagcatcga gagtagttac aggggtattg atttgaagac
    12001 ttatgtgtcc ggaagttata ataatcctac actcaatgat ttgaattggg tgcctggtgg
    12061 caatatacat ttgaaaccgg agaaaggaat aaatgccgga gttaccattt ccaaaaaatg
    12121 gtatatttca cccatagaga acaaaactac gcttgaaggc ttttatcata atatacaaga
    12181 ctggatacag tgggtgccgc agggaagtat ctggagtcct gtaaatttca agcacgtcat
    12241 cagttcaggt gttgtttttg accaacagtc gttattacaa acaggcagca gtcattttat
    12301 actaaatata aagggcactt acacacatgc tgtcaacatt tctaaattgc cggaggctaa
    12361 ctacaacaaa caattggctt atatccccgg tttgacaggg atgttttctc taagttatat
    12421 atccggtagt aagctttcat taagggccga actgctgtcg gttggcgaaa gatatacagt
    12481 attcgatcag tctgcgtcgt tgccatctta tgcaatcgta aatctgtatg ccggtaaaaa
    12541 aatagtgctg aagaaaactg aattattgcc atatctgcag gtcaataacc tgctgaataa
    12601 atattatgag tctgtagcat tcagacccag agaaccattg cattttttaa tcggatttaa
    12661 aataaatatt ttatgaaaaa aattttaatt gtttgtttgg cgttatccgc atttttctcg
    12721 tgtaaaaagg atgacccgat tacacccggt aagtacgggg cagggttgct tattctgaat
    12781 gaaggtccgt ttggtaccgg ctcgggtaca ataggatttt gggaccgttc ggataaagca
    12841 ccggtcaatg atgtgttcga aacagaaaac acaggtgcca agcttggcaa tattcttcag
    12901 tcgatgacac agtctggcga aaaattctat tttgttgtca ataactcgaa caaagtcgtt
    12961 gttaccgata aaaacttcaa gtatcttagt gaaataagcg gtttgcagtt gccaaggtat
    13021 ttggttgtaa agggcagcaa ggcttatatt tcacagtggg gaagcgacgg ccttacaggt
    13081 tcactggttg tagccgacct tgctacaggt cagttgacaa agaccattcc acttggtaaa
    13141 gggcctgaaa atatgctcat taaaggcgac aaactctacg tttcaatggt tggtgggtat
    13201 gaccgtgaca acagagtaat ggtggttgac cttgtcggag aaacagtttc gaagacaatc
    13261 acagtggatg acaatccttc caacattata gattacaatg gtcagatact tgtattgtgc
    13321 aaaggtttta cggattatgt caatccggca aacagtacca atggcaagat tgtagaaata
    13381 tcaaacgatg cagtatcggc tacgtataat ctgtcagaac aagcagataa cctaatcagg
    13441 aagggcaata gctcaactct ttattttaca cttaatggca agccatccaa atgggaatac
    13501 aaggctgcta attttgaaac tttacaaggt gcacctttta tctacaagct ttcctatgat
    13561 gatgttgcag gcaaagtgct catgtcagat gcagtagatt ttgcgtctaa aggaaaaata
    13621 ttggtagcag acgaaaacct gaacgtgagc aaaaccatcg atgccggtat cattccttca
    13681 ttggtctatg tcataaaata atcagttctc aggttcattt agaatctttt gaatttcttt
    13741 ttctgtgctt cggagtctgt ttttacaata agtgaccagc tccgaagcac gttttatttt
    13801 ttcggtgagc tcttcgactc cggtttctga attttgaagg tcggagagga ttttctggag
    13861 ttcgttgtat gcctggtcgt aagtcagatt cttttctttc attatatttt ggtaatgttt
    13921 ccggtcattt tgccgatgat tgaataaata tttatttcgt cacctacttt aggtttattc
    13981 tttttgccaa ttcttgcatt attgtgttct acaatgctgt atccacgttt gaaatgagcc
    14041 tcaggtgata attggctgag caaggttgaa atatgatcaa gttgggttaa attgatgttg
    14101 agtttgtgtg ttgctaccat cggtatttgg ccaaaaataa ggtcgagtga tgattcggcc
    14161 ctcgagatgg ctgaggtggc tatccagttg agtctttgtt tagcataatc aagaacttcg
    14221 gtgtgttgtt gcagattatt ctgtatttgg atggatatac tgcggccgag ttcgattatg
    14281 ttgctttcat aacgcatgtt gtgctggatg atgtattctg caacagcagt tggtgtctta
    14341 agtgatgtgt gtgcaaccat atcagcgatg gactggtcga tgtcgtgccc gataccggta
    14401 aataccggca atggcatacc ggcaatggtt tttgctatca gtaggttgtc aaaatctgaa
    14461 agatctactt ttccaccacc acctctgatg agaacaacac agtcaaattt gtctttttgt
    14521 ttgattattt ctttcagatt ttcagttact tcgggttcta cattctgtcc ctgcatagct
    14581 gaatggaaca atgttacctc atgatggtag ccaaagatat tgtcttgcag ctgatttttg
    14641 aaatcctgcc agcctgccgc attctcggat gaaatgacag ctattcgttg cattacataa
    14701 gggagggcaa tctccgcgtt tttgcccaac aggttttgtt ttgcaagctc gttaaggatt
    14761 tcctgtctgc ggagggcgag ttttccaagt gtgaatgaca agtcgatgtc ttcaactata
    14821 agtttgaggc catatttttc gttgtattct acttttactt taatgagtat ctctttcccc
    14881 ggctgcagaa ccgactcttt ttcggggccg agttttttac tgatggccat taattgggtg
    14941 cgccagatga ctgccgatgc ttgggctatt atttcatgat cgaattccga attttctatt
    15001 aattcaaggt aggcatggcc tcttgattct cggaaggaag cgatttcgca gttgatccag
    15061 atactttcac tgaagttgag tgctattact ctgcggatgt attcgttgag ttggtataat
    15121 ctgagtgact gattcaatgg aaggatttat atttgcaaag ttatctgttt attttgaaat
    15181 aattgcagtc atgctcagta catttgtcct aggctttgga tgaatggttt gggtaatgtt
    15241 attttcttat ttatgttaga aaaagtgatt gacatgaacg aggaaaattt tctaaaaaca
    15301 atcaaaacac cctggaaatt caggtggtac cttttcacca aattgccact tgcttggttg
    15361 atgggcatcc gtttgcttgt tatcgaccat agccattgta cggtcatcct gccattcaag
    15421 aagcgcaacc tcaatccgtt tagaagtatg tacttcgctg ctcaggtagc tgcagccgag
    15481 ttgagtacag gcatattgtt ggtgatggca cgcgaggctg ccggtgatgt ttcgatgctt
    15541 gtaagcagaa tcgaatctga attttacaaa aaagccgtta cagatttacg cttcacctgt
    15601 aatcagggag aaatgataac tgacatcatc cgtaaagcag tggtatccgg tgacccaatg
    15661 accatcgtag cggagtcaat aggtaaggat gaacatgaag ataccgtaag cataacgcgg
    15721 ataacctggt cggtgaaacg cagaaagaaa taggctgatc agttgacaaa tttgattttg
    15781 tattcggctt gtatattgcc ttttttaata ttctcgagtt ttttctggat gagttttttc
    15841 ttcaacggtt tcagtttttc aacaaaaagt acaccttcga tgtggtcgta ttcgtgctga
    15901 atgactcttg cattgaggcc atcaaaggat tttgtgtgtt tggtgaaatt ttcgtcgtag
    15961 tattcaatgg tgatattcgg ttgtcgcata acatctcctc gtacaaacgg gatgctaagg
    16021 caaccttctt cgtaggccca ttcttcacct gactcttgaa gtatgcgggc attgataaaa
    16081 acggttttga tgccatcgga tgctttatct tcatccatgg tttgtattgt atcaacaata
    16141 aagagcctga tgtctaaccc tatctgtggt gcggcaagac ctacaccgtg ggcgttgtac
    16201 atagtttgcc acatattttc cagcagtgtc gtaaggttcg ggtagcttga atctatatcg
    16261 gtagctactt tttttaacac cggctgacca taagcatata tcggtagaat cataagttta
    16321 ttgatattga cctgcaaata taaggaaaat tacctaaaag tactgtgcct ttcctatatc
    16381 cggctatctt ggtaggcttt agagtgcaaa aattcttcga ggatgattac ggcacttacc
    16441 ttatctatca gtgatttgtc ttgtcttttt tgtctgccaa taccgctttg caatattacc
    16501 tgcgatgcct gacgtgaagt aaaccattcg gcttgccgga agattttgat ctgtgggaat
    16561 ttttgtgcaa aattatttag aaacacgtca atctcgttgg tgaggtaagt atcatttccg
    16621 tcgagatgtg ttgggtaccc gatgaccagc atagatactt gttcggtttc gaaatatttt
    16681 tcaaggaatg tcatcaagtc tttggtctgt aaggtttcca ggccattcgg aacgatttgg
    16741 agtgggtcag taacggctat tccggtgcgt tttgatccat agtcaagtgc aataattctg
    16801 ctcatctgtg gtatatagtg tttcggtttt ttggttgcag gaatattgtg ttattgtttt
    16861 tcgccgctga atacagtatt gtcaagcttg ttttttacag ccagactttg cttgtcgggg
    16921 cttattttac tccatttgat taaccacgac tgagagtcgc cctgaatgtc cgaagtaccg
    16981 gattctgata aagaaatatt gtcttgtgca aaagccggat ttccgttcag atgctccatt
    17041 tttacagtaa tattatcgat tttggtaagt ttgacctgag tagtgacata gttggaagtg
    17101 cctgttatga gctgaccttg gtaggtgcct tcaacctgtg ctgtcaggtc gggttggtca
    17161 ggtgctgatt tcgagcacga agctattgta aatgataata gagcgaatac aaaaagataa
    17221 actgtttttt tcatgagata attttcaaca aatatacgaa aaatggagat taattcaata
    17281 ttcaaattcc cgtagggaaa aaaattaaca caaccgaaaa atcagttaag catattgaag
    17341 gtaaaaatta ataaaaaaaa gtccctgctc tttcgggcag ggaccttaca accaattcga
    17401 aaattggtgt caaccaacaa aaaaccttat tctatgatga tcatcttcat cgttttagat
    17461 tctgttgcag tattcagctg atagtagtag ataccgccgg caccgatttc agctttgtga
    17521 agcttaattt ggttgaggcc tttgttgaaa tcgccttcta tcattttgac tactttacct
    17581 tctacattgg tgattacgag ttgagcatat tcgtcctgag gcaggtagaa gctgatattg
    17641 gtaacatcac caaacggatt cggattgttt
//
LOCUS       DAOQQY010000024        17599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_998_length_17599_cov_5.851687, whole genome shotgun sequence.
ACCESSION   DAOQQY010000024 DAOQQY010000000
VERSION     DAOQQY010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17599
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_998_length_17599_cov_5.851687"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(245..883)
                     /locus_tag="PLE23_02555"
     CDS             complement(245..883)
                     /locus_tag="PLE23_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter"
                     /protein_id="HMX82177.1"
                     /translation="MAELLPFALLALTSFFTLINPFGTMPVFLSMTAELSEKDRKKTA
                     KKATLVALITLIIFAFTGQLLFSFFGISVNSFRVVGGIIFFIMGMDMLQARLGQVRIK
                     DAEVKSYITDISITPLAIPMICGPGAITNAIVLMDDAHSFARKVILILSIVVIMFLTY
                     LVFYSSSKLVKLLGQTGINVMMRIMGLIVMVIAVEFFFSGLKPILLDILRSK"
     gene            complement(950..1513)
                     /locus_tag="PLE23_02560"
     CDS             complement(950..1513)
                     /locus_tag="PLE23_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMX82178.1"
                     /translation="MFADTCHAIMAPAGEAWRKSIGLDPKLELYSSDQSHPALEGSYL
                     TACVIYEVLFGKSVIGNTYTAGMLKYPTEFLQQVADDVVNDSLEVWNIGKFDPCKITT
                     SEGHFKEKNTRIYPNPVKDELIISSSGEMDENSIKIRITGMTGRRYELKDLFSDKVDV
                     SHLPAGMYFLEILTGNIKKEALKFIKQ"
     gene            complement(1532..2032)
                     /locus_tag="PLE23_02565"
     CDS             complement(1532..2032)
                     /locus_tag="PLE23_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82179.1"
                     /translation="MNRRIKILNTVCFVSIFMLSIIETATAQTGKKIQVLFLGNSYTS
                     VNNLPLMISNLASAAGDTLVFESNLPGGYTFQNHYSNNTSKTLIKSRHWDYVVLQAQS
                     QEPSFPPDQVAFQTLPYAIGLNNLIISNDSCTKTVFYETWGRKNGDASNCAFYPPVCT
                     YQGMQD"
     gene            2264..3919
                     /locus_tag="PLE23_02570"
     CDS             2264..3919
                     /locus_tag="PLE23_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMX82180.1"
                     /translation="MNYTIRQLLIFIFLTNIAVAYAQMKNNNDRPKLVVGIVVDQMRW
                     DYLYKYYQHYNNEGLKRMLNEGFSAENTLIPYIPTYTAIGHTSIYAGSVPAIHGIAGN
                     TFYIKETQSKMYCSQDDSVKSVGNDPENKGGKMSPKNMLSSSVTDELKLATDFRSRVF
                     GVALKDRGAIFPAGHFADGAYWMVDGNWITSSFYMNDLPSYVKNFNKADFTSAYLSKG
                     WQLAKPSTSYMSRINDDNRYEERFLDSVKVTFPIDLSTISSKKGRDVIKSTPFGNTIT
                     LQFAKELITQENLGNNPAGVPDFLAISLSSTDYIGHQFAINSLKIEDTYIRLDKDLGD
                     FFNFLDTKIGRGNYTVFLTADHGAAHNPQYILDEKGNGGYFMKRELQQDLNNLLKPEF
                     GTEDLIYNIANNQVLLNHKLIESKNLDTERIKEKLVTALKKREGVSFATDMERLQVAS
                     MPEWIKTMAINGYNYKRSGDILIIFDPQWIENYRDKTGTTHGAWNPYDSHIPLVFMGW
                     GIKQGNTHAPVYMTDIAPTIAALLHIQAPNGCIGKPITELLDK"
     gene            4009..5739
                     /locus_tag="PLE23_02575"
     CDS             4009..5739
                     /locus_tag="PLE23_02575"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HMX82181.1"
                     /translation="MNGGQIIAKVLQNHGVKFLFTLCGGHISPIFVGAEQLGIRVIDT
                     RHEVNAVFAADAVSRLSGVPGVAAVTAGPGVTNTITAIKNAALAQSPLILLGGTAATI
                     LKGRGSLQDIDQISILKSVCKQCISIHKVKEIVPAIEQAFRLAQSGVPGPVFVECPID
                     ILYDEELVRNWYGAKSNETPPKNLRERALRWYINFHANRLFAGKETVKFNSPPIKPEI
                     TATNPNDIAAAERLLKKSKKPLLITGSGAMMQPQKSQELADSVKKLGIPVYLSGMARG
                     LLGKEDSLQMRHKRKEAIKAADLIILAGVPNDFRLDYGNHIGGRTFISINRSKEDLYK
                     NKKPTLAIEADPAETLISLASKYTAEHTSWIDELRQLNQKRDDEIISQANETLNGGIN
                     PLALFHNLDDTLGDNTIFVADGGDFVGTAAYTLRPRAPLSWLDPGVFGTLGVGAGFAL
                     GAKLVFPEKDVWIIYGDGSAGFSLMEYDTFARHNLPVVSVIGNDACWGQIARDQVEFL
                     KSDCAMNLAHSHYELIAKAFGGDGMRVHNMEEWRDAVSKAKENISDGKPYIINSILGK
                     SDFRKGSISV"
     gene            5773..6321
                     /locus_tag="PLE23_02580"
     CDS             5773..6321
                     /locus_tag="PLE23_02580"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HMX82182.1"
                     /translation="MSAAFPGVNNSLLLVIDVQDRLMPVIHDGDKTIANIQKLIDGAA
                     ILGLDTMITEQYPKGLGNTIAELRFDEKTPVLQKNTFSCMLEQSVITQLSIYKKEHLI
                     LCGVESHICVLKTAIDAVSHGFTVHVVADAVSSRSPESKQLAFDRMRQSGVFIVSLEM
                     ILFMLLDCAGTEEFKAISKLIK"
     gene            6328..8115
                     /locus_tag="PLE23_02585"
     CDS             6328..8115
                     /locus_tag="PLE23_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMX82183.1"
                     /translation="MEIKRLFDYLTLHTDKRPETEFVSGKEIKANGTKEWRHYTFREV
                     KDISDKISQLLINLGLKKEDKIAIISANRPEWNFADLGSQQIGIINVPMYPTISEADY
                     EFIFKDANVQYAFVGDESIYKKVEPLLGRVGSLKGIFTFDRIAGADYLPEVLDRIDHI
                     DQDEIENRKRLVDADEVVTYIYTSGTTGNPKGVMLTHNNIVSNLTSVARMLPFKEGKS
                     LSFLPLCHSFERTVFYAYLMMNIHIYYAENLETIGDNLKEVQPFCFSTVPRLLEKVYE
                     KIIAKGHELNDIKKAIFFWSVRVGSQYELGVDKGRWYNLKLSIARKLVFSKWLEALGN
                     HVEFIVTGAAAMQPRLITIFCAAGIHIIEGYGLTETSPVITINRFPEQERCLGTVGLP
                     ISGVEVRLGEDGEIIARGPNIMKGYYNRPDLTVEVIDKDGWFYTGDIGVWVEHFGRKF
                     LKITDRKKELFKTAGGKYIAPQAVENKMKESRFIEQIMVVGGDDKKYIAALIVPSFVQ
                     LRAWAEENEIALTTIEELVKNQFVNELLNKEVTRLNHSFGKWEQIKKIKLLPDEWTID
                     SGFLTPTLKLKRRVLADVFKNDIDALFKE"
     gene            complement(8148..8636)
                     /locus_tag="PLE23_02590"
     CDS             complement(8148..8636)
                     /locus_tag="PLE23_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX82184.1"
                     /translation="MTYHSSDIHIREYTENDKNTVLGLLRMNIPAYFAPKEEADFSNY
                     LDTLIELYYIIECEGKVAGCGGINFKDQITVGVISWDVLHPDYQGKSLGTRLLKHRID
                     ILSGISSVRKISVRTSQMAYEFYKKNGFVLKQIETDYWAEGYDMYYMEYQPSEAPLAQ
                     PA"
     gene            complement(8769..9797)
                     /locus_tag="PLE23_02595"
     CDS             complement(8769..9797)
                     /locus_tag="PLE23_02595"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HMX82185.1"
                     /translation="MEQTPMWRLALKGVIPPMDKQAWDKLSFVSRFLISTRASVLIMT
                     VLSVFIAILFAIGHHQFDLVKALILLVSITLAHATNNILNDWSDWKRKVDEEGYFRSR
                     YAVEPLTHGLMTGKQALTQAFITGGLAALGGIYLISQVPDVWLGIGLFVAGLFFLLFY
                     TWPLKYLGLGEVAVILVWGPLMIGGGYYMLSGVWSNEVMLAGLPYALGVTGVIFGKHI
                     DKYEEDKAKGIHTLPVILGERNSRYVMIAAVLLQYLITTYLVFTGFFHPIMLIVWITF
                     LGVPKLRAKIPVWNLIKTFSKPRPTEAPAGWRSWPLYFVSIAFVQNQRFGIIFVLGLI
                     IQTILKLL"
     gene            complement(10239..10889)
                     /locus_tag="PLE23_02600"
     CDS             complement(10239..10889)
                     /locus_tag="PLE23_02600"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HMX82186.1"
                     /translation="MYKLPYFNEQNPQLVREFMENHPFVIMTGSFNNGQQVVTQVPVL
                     LEMRDDKLYIQGHIMRKTDHHLAFAENPKVLIVFVGPQAYISSSWYTDQASGSTWNYM
                     SVQASGDIRFMTDDEMVSFMRKLTLRFENNNPHSSALYEKLPEDYTRQMLPGIIGFEV
                     MVEKLENVFKLSQNKDEASYTNIIKHLHTQGGDAALLAVEMEKRQAHIFQKDQEHQ"
     gene            complement(10999..11865)
                     /locus_tag="PLE23_02605"
     CDS             complement(10999..11865)
                     /locus_tag="PLE23_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMX82187.1"
                     /translation="MKSNLILLMGLCLCFQLAAQKKPMNSQVKPLTIGEIHEIYSNEL
                     AENRKLNIYLPDGYNPNDTTRYPVIYVLDGSIDEDFLHIAGIVQYNNFPWIDRVPPSI
                     VVGIANVNRRRDFTSPSNFEIDKKHAPAGGGSAKFISFIEKELEPYIESRFRTKADRT
                     IVGQSLAGLVATEILFTKPTLFNRYIIVSPSLWWNDGSLLKVQADVLKADFTQPTDIY
                     IGVGKEGLTPGLDNHVMEVDANLLYDKILQGKSKNVKVYFDYLPAENHATVTHPAVFN
                     AFRMLYPDKHSK"
     gene            12289..12717
                     /locus_tag="PLE23_02610"
                     /pseudo
     CDS             12289..12717
                     /locus_tag="PLE23_02610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
     gene            12802..13365
                     /locus_tag="PLE23_02615"
     CDS             12802..13365
                     /locus_tag="PLE23_02615"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMX82188.1"
                     /translation="MKENGRTEARKHENVSIIFTDFKDFTSLTSTMAPTILMEELNEI
                     FGYFDDILEKYKIEKIKTIGDAYFAVCGLSEGVENHALQCVEATKEMFGYLALRNKTS
                     QIKWRMRAGIHSGPVVAGVVSKKKYAYDLFGDTVNTASRMESNGKPGKINISEATFQL
                     IKEWISCTPRGSIRTKGKGGMNMYFVD"
     gene            complement(13405..13779)
                     /locus_tag="PLE23_02620"
     CDS             complement(13405..13779)
                     /locus_tag="PLE23_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82189.1"
                     /translation="MKSKILNLLLITTSLFGYLEWGGGNHSFLYEAEAEFLSKLFASP
                     GSVFHPLTILPFVGQLLLLVSLFQKEPCKVLSYIGMAGTGILLVFMFVVGLLSLNFKI
                     VLSTLPFLIVLILAWRHHRTRA"
     gene            complement(13847..14290)
                     /locus_tag="PLE23_02625"
     CDS             complement(13847..14290)
                     /locus_tag="PLE23_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX82190.1"
                     /translation="MKPIAPDEDKSDPMFDSEYCRQLLSMYNQFYPKTGFILPWVGYF
                     VMHHEQIVGSCSFVGPPNDGRVEIAYWTFSEYEGRGIASFACKELIKIAKMHQPGIII
                     TANTAPEHNASTKILEKNGFRYSGIVQDDDIGDAWQWLLRPENTL"
     gene            complement(14418..16094)
                     /locus_tag="PLE23_02630"
     CDS             complement(14418..16094)
                     /locus_tag="PLE23_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX82191.1"
                     /translation="MPHPKSRMFRKLVALLKFELEKLPENQPERNKRNIQQSRRRFIE
                     HSAKAAIGVSLSVSMPAFLSSCRNTPSSASADTDAPPVVGEKPLDIAILGGGIAGLNC
                     ANHLLESGHDFKIFEAQKRVGGRILTHYNDPLGLGIYPEFGGDFIDTGHEDMLALAKE
                     FGLELYDLEAEMKKDGLVKDVYLFNDKIFTEKDIIREFRKIAPKIAADYKSLGENYDT
                     EAAKKFDLMPLSEYFASLKCAKWLKDVLIAGFVSEFGLDCNEQSTINFLDLIDPDTSG
                     GFKIFGESDERFRIKGGNSKIIEGLEKKIGSDRILKRYEVTEVSEQDDGSYKISFTDQ
                     KPVLAGMVVCTIPFTILRKIKMSLKNISPEKQKCINELGYGMNTKLVLGYNERSWRSK
                     ENKAMGYLFHKDIVNGWDSSYNKLDNSDKAAFVCYFGGKFSVDLDGIAVRNPMSPPQH
                     TWRTQLPEAKVDEFVNVLDKAFKNSKKHFNNKHIFVNWIDFPYTKGSYACYKPGQWTT
                     IAGLEMQPVGKFLFAGEHCSAMFQGFMNGGAETGRHVAEAIKASVGAGTK"
     gene            complement(16259..16729)
                     /locus_tag="PLE23_02635"
     CDS             complement(16259..16729)
                     /locus_tag="PLE23_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX82192.1"
                     /translation="MDKYKCLKILPDDEMYISIIASWYFHEWGIAEADTIDRIKGLGT
                     DNEEFQVLLTLGGKPIATGGLYNHVGLLNKLPHLKIHKHWLALVYTIPEYRGNGAGSA
                     ICMEIEKMAGDLGCSVIYLFTHTAENLYQRLGWNTVQRLTLADKNIAVMKRDVL"
     gene            complement(16862..17431)
                     /locus_tag="PLE23_02640"
     CDS             complement(16862..17431)
                     /locus_tag="PLE23_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMX82193.1"
                     /translation="MQSENLIEKLIDQTKQIISQTEMLKDESINMLSWRETENSWNIL
                     ECLEHLNLYGDFYLPAIDNSINKSVAKPDAEFRSGLLGAYFAKSMLPGQKMNKMSTFK
                     DKNPLHANLDKQVIDKFCAQQIKLLELLERSGKVSLNKVKVPTSISRLISLKLGDTFQ
                     FYINHIIRHLSQIERIKAASRNVQSTMVE"
BASE COUNT         5407 a         3653 c         3522 g         5017 t
ORIGIN      
        1 taccgtatgc catttgcggt gccattcgaa atgctctgct tgctctgccc agaattgctc
       61 cggatgctca atacttcttc tgtattcgtc ctgatattga ctcagtgaat taatctgtaa
      121 catcatgatt ggttagtatt aggctgttat ttgcgaatgt gttccattcg gcctgataaa
      181 gttaatgaat ttttccctaa tcagcgctat caaatagtta tttaacaata ataagaaaat
      241 aatgctactt acttcgtaga atatccagga gtattggttt caagccacta aaaaagaatt
      301 ccaccgcaat caccataact atcagaccca tgatgcgcat cataacattg atgcctgtct
      361 gaccaagcag ttttaccaat tttgacgaac tgtagaaaac cagataagta agaaacataa
      421 tgacgacaat cgacaaaatg agtatgactt ttctggcgaa tgaatgtgcg tcgtccatca
      481 aaacgatggc attggtgatg gcacccggcc cacagatcat cgggatggcc aaaggtgtaa
      541 tagaaatatc tgtaatgtag ctctttactt cagcatcttt tatacgcacc tggccaagac
      601 gggcctgcag catatccatt cccataatga agaaaataat accgcctacc actcgaaaac
      661 tattgacaga aataccgaaa aaactaaaaa gcaactgtcc ggtaaatgcg aaaattatga
      721 gtgtgataag tgcgacaagt gtcgctttct tggcagtttt tttgcggtcc ttttccgaca
      781 attcggccgt cattgaaaga aaaaccggca ttgtaccaaa agggttgatt agtgtaaaaa
      841 aggatgtaag agccagcagc gcaaagggta gtaattctgc catggtattt tgttgcttgt
      901 ttgtttattg ttaaattttt atgcagagta tcccgcattc aattcgcctt tattgtttga
      961 tgaattttaa tgcttctttt tttatgtttc ctgtcaaaat ttcaagaaaa tacattccgg
     1021 caggcagatg agatacatcc accttgtctg aaaatagatc tttgagttca taacgtcgtc
     1081 ctgtcatgcc agttattctg attttaattg aattttcgtc catttcacct gaagaagaaa
     1141 ttatcaactc atcttttacc ggattggggt aaatgcgtgt atttttttct ttaaaatgac
     1201 cttcagaagt ggttattttg caagggtcaa atttgccaat gttccagacc tcgaggctgt
     1261 cattcaccac atcatctgct acttgctgaa ggaattcagt cggatacttc aacataccgg
     1321 ctgtataagt attgcctatt acacttttcc cgaacaaaac ctcataaata acacaagccg
     1381 tcagatagct gccttcaagc gccggatggc tttgatctga actgtagagc tcgagtttgg
     1441 gatcaagccc gatagatttg cgccacgctt caccggcagg agccataatg gcatggcagg
     1501 tatctgcaaa caacttgtaa gattccttca gtcagtcttg catgccctga tatgtacaca
     1561 caggcggata aaaggcacag ttggatgcgt caccgttttt tcttccccag gtttcgtaaa
     1621 atacagtctt ggtacagctg tcgttactta ttatcaaatt attcaaccca atggcataag
     1681 gcaatgtctg aaatgccacc tgatcgggtg gaaaagaagg ttcctgactt tgtgcctgga
     1741 gcacaacata gtcccaatgc ctagatttta taagggtttt actggtgttg ttgctgtaat
     1801 gattctggaa agtataacca cccggcaagt tgctttcaaa cacaagtgta tcacctgctg
     1861 cacttgccag gtttgatatc atcaaaggca gattattgac acttgtatag ctgttgccaa
     1921 gaaacaatac ctgaatcttc ttacctgtct gtgccgtagc tgtctcgatt atagataaca
     1981 tgaagatgct tacaaaacaa actgtattaa gaattttaat tctcctattc ataacaatga
     2041 tatttataac tgaaaaaact gtctgacgaa tatttaagtg caaaattcgc aaaatacaag
     2101 caaatcaaac acatacgcct tcgaatttgt tgtttgcaga gcaccttttg atgcccaaca
     2161 acaaaaatgt attgtcaaat tgtgtttaaa attgattatc tttgaaattt atttctgaaa
     2221 aattgctatg aaaaagagct gtaacaaaca tttcaataaa aaaatgaatt acaccataag
     2281 gcaactatta atttttattt ttctgaccaa catagctgtg gcttatgctc aaatgaaaaa
     2341 caacaatgat cgcccaaagt tagtggtagg tatcgttgtt gaccaaatgc gctgggacta
     2401 tctgtacaaa tattaccaac attataacaa tgaaggatta aaacggatgc tcaatgaagg
     2461 attctcagca gaaaatacct tgattcccta cattccgact tatactgcca tagggcatac
     2521 aagtatttat gcaggaagtg ttcctgccat acatggtatc gcaggcaata cattttacat
     2581 aaaggagacc caatcaaaga tgtactgctc acaggatgac agcgtcaaaa gtgttggcaa
     2641 tgaccctgaa aacaaaggtg gtaaaatgtc gccgaaaaat atgcttagct catccgttac
     2701 tgacgaactg aagttggcaa cagatttcag gtcaagagta ttcggagtag cacttaaaga
     2761 cagaggtgca atatttcctg ccgggcattt tgcagatggt gcctactgga tggtggacgg
     2821 caattggatt acgagtagtt tttacatgaa tgatttgccc tcttatgtaa aaaactttaa
     2881 caaggcagat ttcacctcag cttatctttc aaagggctgg caacttgcca aaccttccac
     2941 ttcctatatg tcgcgtatta atgacgacaa tcgttatgag gaaagattcc tggacagtgt
     3001 aaaggtcact ttcccaatag acctaagcac gatttcatct aaaaaaggcc gtgacgtaat
     3061 caaatcgaca ccttttggca ataccataac acttcagttt gcaaaagaac ttataacaca
     3121 ggaaaatctg ggtaataacc cggcaggtgt acccgacttt cttgctataa gcctttcgtc
     3181 aacggattat ataggacatc agtttgccat caattcatta aaaattgaag atacctatat
     3241 caggctcgac aaagacttgg gcgatttttt taatttcctc gatacaaaaa ttggacgggg
     3301 caattacaca gtatttctca ctgctgacca cggagcagca cacaatcccc agtatatttt
     3361 ggatgaaaaa ggcaatggcg gatatttcat gaaacgcgag ctgcagcaag atctgaataa
     3421 tctgttaaaa ccagaattcg gaaccgaaga tcttatctac aatattgcca ataaccaggt
     3481 gttgctcaat cacaaactga tcgaatcaaa aaacctggat acagaaagaa tcaaggagaa
     3541 acttgtaacc gcgctgaaaa aacgagaagg cgtatccttt gccacagata tggaaagact
     3601 ccaggtagct tccatgccgg aatggataaa gaccatggcc ataaacggtt acaactataa
     3661 gagaagtggt gatatcttga ttatcttcga tccgcaatgg atagaaaact accgagacaa
     3721 aaccggcacc acgcacggag cctggaaccc ttatgacagc catattccgc ttgtatttat
     3781 gggttggggc atcaaacagg gcaacacaca tgctcccgtt tacatgacag atatagcacc
     3841 taccattgca gccctcctac atatacaggc acccaacgga tgtattggaa agccaattac
     3901 agaattgctt gataaataaa agtacaccga aatttaccat tgaattggta atatccttat
     3961 tattgtagag tcattgcatc gaatggcctg aaaaattaaa gacaggatat gaatggtggg
     4021 caaataatag ctaaagttct gcaaaatcat ggcgtaaagt ttttgtttac actttgtggc
     4081 ggtcatattt ctcctatatt cgtaggtgct gaacaactcg gtatcagggt gattgacaca
     4141 cgccatgaag tcaatgctgt ttttgctgca gatgctgtct caaggctgag tggtgtacca
     4201 ggtgtagcag ccgtaacagc agggcccggg gtgaccaata ccattactgc catcaagaat
     4261 gccgctcttg cacagtcacc tctgatattg ctgggtggta cggctgccac tatactcaag
     4321 ggcagaggca gccttcagga tattgaccag atatctattc tgaaatcggt atgcaagcaa
     4381 tgcataagca tccacaaagt aaaagaaatc gtccctgcta ttgaacaagc attcagactt
     4441 gcacaatcgg gtgtaccagg ccctgtattt gtagaatgcc cgatagatat actttatgac
     4501 gaagagctgg tgcgcaactg gtacggagcc aaaagcaacg aaacaccacc taaaaacctt
     4561 agagaacgag ccctaaggtg gtatatcaat tttcatgcaa accgattatt cgccggaaag
     4621 gaaaccgtaa aattcaactc gcctcccata aaaccggaaa ttacggctac aaacccaaat
     4681 gatattgcag cagcggaaag attactcaaa aaatcaaaaa aaccactgct cataaccggt
     4741 agcggagcca tgatgcaacc acagaaaagt caggaattgg ccgattcggt aaaaaaattg
     4801 ggcattccgg tttatctgag tggaatggca cgcggcctgc taggcaagga agatagctta
     4861 caaatgcgac acaaacgcaa ggaagccatc aaagctgctg acctcatcat tttagcagga
     4921 gtacccaacg acttcagact cgattacggc aaccacatag gcggccgtac attcatttct
     4981 atcaacagaa gcaaggaaga tctgtataaa aacaaaaaac ctacactggc catagaagca
     5041 gatccggcag aaacactgat aagccttgcc tcaaaataca cagccgaaca cacttcgtgg
     5101 atcgatgaac tccgtcaatt gaatcaaaaa cgtgatgatg aaataatcag tcaggcaaat
     5161 gaaacattga atggcggtat caacccactt gcattatttc ataaccttga cgacacactt
     5221 ggcgacaata ctatattcgt tgctgatggt ggcgattttg tcggtacggc agcctataca
     5281 ttacggccac gtgctccgct gagctggctc gatccgggcg tatttggtac acttggtgta
     5341 ggagcaggtt ttgcattggg agccaagttg gtatttccgg aaaaagatgt atggattatc
     5401 tatggtgatg gtagtgcagg attcagcttg atggaatacg atacattcgc aaggcacaac
     5461 ctacctgtag tgtcggttat aggcaacgat gcttgttggg gacagatagc acgagaccag
     5521 gtcgaattcc tgaagagtga ctgtgccatg aaccttgccc acagccatta tgaactgata
     5581 gcaaaagctt tcggaggtga tggtatgcgg gtacataata tggaagaatg gagagacgcc
     5641 gtaagcaaag caaaagaaaa tatttcagat ggaaagcctt atatcattaa ttcaatatta
     5701 ggaaaatcgg atttcagaaa aggttcaatc agtgtgtaaa atcattattt aaccatcaaa
     5761 acatttttga aaatgtcagc agcatttccc ggtgtcaaca acagtcttct actcgtcatt
     5821 gacgtacaag accgccttat gcccgtcatc catgatggag acaaaaccat agccaacatc
     5881 caaaaactca tagacggtgc tgccattctc gggctcgaca ccatgattac agagcaatat
     5941 ccgaaagggt taggcaatac catagcggaa ttaaggtttg acgaaaagac acctgttctc
     6001 caaaaaaaca ctttcagctg catgctcgaa caatcagtca ttacccaact ttcaatttac
     6061 aaaaaagagc atttgatact atgcggtgta gaaagtcaca tctgtgtgct aaagaccgca
     6121 attgatgctg tcagtcatgg gttcactgta catgtagtgg ctgatgccgt atcatcgcgt
     6181 tcgccggaga gcaagcagtt ggcctttgat agaatgcgcc agtcgggtgt gtttatcgtt
     6241 tcacttgaga tgatactgtt catgctgttg gactgtgcag gcaccgaaga attcaaagca
     6301 ataagtaaac ttatcaaata atctgacatg gaaatcaaaa gactgtttga ctacctcaca
     6361 ttacataccg acaagcgccc ggagaccgaa ttcgtttctg gcaaagaaat aaaagcaaac
     6421 ggcacaaaag aatggcggca ctatactttc agggaagtta aggatatttc tgacaaaata
     6481 agccaacttc tcatcaatct tggtctgaag aaagaagata aaatagccat catcagtgcc
     6541 aaccggccgg agtggaattt tgcagacctg ggctcacaac agataggcat catcaatgta
     6601 cctatgtatc cgaccatttc tgaagctgat tacgaattca ttttcaagga tgcaaatgta
     6661 caatatgcct ttgttggtga tgaaagtatt tataaaaaag tagagccact gctagggcgt
     6721 gttgggtcac ttaaaggaat ttttaccttt gacagaatag ccggagctga ctatttgcct
     6781 gaagtacttg acaggattga tcacattgac caagacgaaa ttgaaaacag aaaaagatta
     6841 gttgatgcag atgaggtggt aacttacatt tatacttccg gcactacagg caatccaaaa
     6901 ggtgtcatgc ttactcataa caatatagta agcaacctga caagtgtggc tagaatgcta
     6961 ccgttcaaag aaggaaaatc gttgagtttt ctaccattgt gccatagttt tgagcggaca
     7021 gtgttttatg cttacttgat gatgaatatt catatctatt atgccgaaaa tctggaaacc
     7081 ataggtgaca atctgaaaga agtacagcca ttttgttttt ctactgtacc ccgattgctc
     7141 gaaaaagttt atgaaaaaat tatagcaaag ggtcacgaac ttaacgacat taagaaagcc
     7201 atcttttttt ggagcgtaag ggttggctca caatacgaac tgggtgtaga taaaggccgg
     7261 tggtacaatc tcaaactgtc catagccaga aaactggtat tttcgaaatg gcttgaagca
     7321 cttggcaacc acgtagaatt tatagtcacc ggagcggcag caatgcaacc aagacttata
     7381 acgatattct gtgctgccgg catccatatc atcgaaggct atggcctcac tgagacatca
     7441 cctgtcatta ccatcaatcg ttttcctgaa caggaacgct gtctcggaac agtaggattg
     7501 cctatttcgg gtgtagaggt caggctcgga gaagatggag aaatcatagc acgtggcccc
     7561 aatatcatga aaggctacta caaccgccct gatttaacgg tcgaagtaat cgataaggat
     7621 ggctggtttt ataccggcga catcggtgta tgggtagaac atttcggcag aaaattcctg
     7681 aaaataaccg accggaagaa agaactgttc aagaccgcag gcggaaaata tattgcccca
     7741 caagccgttg aaaataagat gaaggaaagc cggttcatag agcagataat ggtagtaggt
     7801 ggtgatgaca agaaatacat tgcagcacta atcgtgcctt catttgtcca gttgcgtgct
     7861 tgggctgaag aaaacgaaat tgcccttacc accattgagg aattggtcaa aaaccagttc
     7921 gttaacgagt tattaaacaa agaagtaaca agactcaacc attcatttgg caaatgggaa
     7981 cagataaaaa aaataaaact actgcccgat gaatggacca tcgactccgg atttctgaca
     8041 cctacactta agctcaaacg acgtgtactt gctgatgttt ttaaaaatga tatcgacgcg
     8101 ctgttcaagg aataaaccca tataacacag gaattaaaat ccgcccttca tgccggttgg
     8161 gctagtggag cttcgctcgg ctgatattcc atgtaataca tatcgtagcc ctctgcccaa
     8221 taatcggttt caatttgctt gagtacaaaa ccattttttt tgtaaaattc gtatgccatc
     8281 tgtgacgtcc tgacgctgat ttttctgaca gagctaatgc ccgaaagtat atcaatacgg
     8341 tgtttgagca ggcgagtgcc caaagatttg ccctgataat cgggatgtaa cacatcccaa
     8401 ctgatgacac cgactgttat ttggtcttta aaattaatgc caccacagcc tgctaccttt
     8461 ccttcgcatt caatgatgta gtaaagctca atcaaggtat caagatagtt gctgaaatcc
     8521 gcttcttctt ttggggcaaa atatgccgga atgttcatac ggagtaagcc aaggactgta
     8581 tttttgtcat tttcagtata ttctcttatg tgtatgtcag atgaatgata ggtcatttta
     8641 ttaactaatc aaaagtaaaa tacttcattt acaagcagaa ggcctcacag acgacaccga
     8701 gagcagcccg gacttgccta aaagacctcc tgcttgcaaa tattagtagc tgtcaggcaa
     8761 aatagccgct atagaagttt caatatggtc tgtatgatca atccaagtac aaaaatgatt
     8821 ccaaaccttt ggttttggac aaaagcaatg gatacgaaat acagtggcca cgacctccat
     8881 ccggcaggtg cttctgttgg tcgaggtttg ctgaatgttt ttataaggtt ccacacgggt
     8941 atttttgccc ttaatttggg tacgccgagg aaggtaatcc agacgatgag cataatcggg
     9001 tggaaaaatc ctgtgaatac cagataagtc gtgatcaggt attggagcaa tactgcggct
     9061 atcattacat atctcgaatt gcgctcaccc aatattacag gcaatgtgtg tattcctttt
     9121 gctttatctt cttcatattt gtcaatgtgt ttaccaaaaa tcactcctgt aacgcccaat
     9181 gcataaggca gccctgccaa catcacttca ttgctccata cgccactcag catataatat
     9241 ccaccgccga tcatcagcgg gccccatacc agaatgacgg ctacttcacc gaggccaagg
     9301 tattttagag gccatgtata gaacagcaaa aagaacagac ctgccacaaa caagcctata
     9361 ccgagccata cgtccggcac ctggctgatg agatagatgc cgccaagtgc tgccaaccca
     9421 ccggtgatga atgcctgagt aagtgcctgc ttgccggtca tcaacccgtg tgtcaatggc
     9481 tctactgcat acctcgacct gaagtagcct tcttcgtcca ccttgcgctt ccagtccgac
     9541 cagtcgttga gtatattatt ggtggcatgt gcaagcgtga tgcttacaag caaaatcaag
     9601 gcctttacca gatcaaactg atgatgccca atggcaaaaa gtatggcgat aaataccgac
     9661 aaaacagtca tgataagtac agatgcacgt gttgatatta aaaaacgact tacaaaactg
     9721 agtttgtccc aagcttgctt atccataggt ggtataacgc ctttcagtgc cagtctccac
     9781 atgggtgttt gttccatttt agttctactt tttgaaattt cagaaaatat tgatagttaa
     9841 ttgatagccc aaagttagca aattattctt tgaattagtg tatttatttc acaaaacatc
     9901 acactatatt ttgctataag tctgagcatt gagtcattcg ggttaaaagg aacgattgcc
     9961 aatgcagggc ttggtaatac tgccattctg gcaatgtacg atgccatatt gcaacattca
    10021 gacatgaatg aataatcata gctatagatg atagtaataa taccatcaac taatatgttt
    10081 gaatatctgc tcagtatttt ttctggtcca ggttattttt gtaaaatatt ggcgcaactt
    10141 acagaaaaat gtcttcgaat atcggcttga tatcagccaa acgactgttc attcattatc
    10201 attcaaattt cacctggctt aaactcatgc cttcattctt actgatgttc ttgatctttt
    10261 tgaaaaatgt gtgcctgccg tttctccatt tcaacagcta acaatgcagc atcaccgcct
    10321 tgtgtatgta ggtgtttaat aatgttggtg tagcttgctt catctttgtt ttgactgagt
    10381 ttgaacacat tttcaagctt ttcaaccatt acctcgaaac cgattatgcc aggtagcatc
    10441 tgcctggtgt agtcttcagg cagcttttcg tataatgcgg atgagtgagg attattgttt
    10501 tcgaagcgca aagtaagctt acgcataaaa ctgaccattt catcatccgt cataaacctt
    10561 atatcacctg atgcttgtac actcatgtag ttccaagtag atcccgaagc ctggtcggtg
    10621 taccaagagg agctaatgta agcttgtggc ccgacaaaca ctataagtac cttggggttt
    10681 tctgcgaaag caagatggtg gtcggttttt cgcatgatat gaccctgaat gtacaattta
    10741 tcatctcgca tctctagcaa tacgggtact tgtgttacta cctgttgacc attgttaaag
    10801 cttcctgtca tgatgacgaa aggatgattt tccatgaact cgcgtacaag ctgcggattt
    10861 tgctcattga aatatggtaa tttgtacatg gctttactat atataaattt acataaattg
    10921 aaatacgact aatctgtccg acttaaaaat ttacattcag acggccatta ggtatttatc
    10981 tatcaactga atcgatcctt atttgctgtg cttgtccgga tacaacattc taaaagcatt
    11041 aaacacagcc gggtgtgtga ccgtagcatg gttttctgcc ggaagatagt cgaaataaac
    11101 ctttacattt ttcgatttgc cttgcaatat tttatcatac agtaggttgg catcaacttc
    11161 cattacgtgg ttgtccagcc cgggtgttaa tccttcctta cctacgccga tatagatgtc
    11221 agttggttgg gtgaaatcag ctttaagcac atcggcttga acttttagca gacttccatc
    11281 attccaccaa aggcttgggc ttacgatgat gtatctgttg aaaagtgtcg gctttgtgaa
    11341 cagaatttca gtagcaacaa gaccagccaa agactgccca acgatggttc ggtctgcttt
    11401 ggtacgaaac ctcgactcga tataaggttc cagttctttt tcgatgaatg aaataaactt
    11461 agctgaacca ccaccggcgg gagcgtgttt tttgtctatt tcgaaattgc tgggtgaagt
    11521 aaaatccctt cttctgttaa cattggctat tccaactaca atggaaggcg gcacacggtc
    11581 tatccatgga aaattattat actgaacgat gccggcaata tgcaggaaat cttcatcaat
    11641 agagccatcg agcacataga tgaccgggta gcgagtagtg tcatttgggt tatagccatc
    11701 cggcaaatag atattcagtt ttcggttttc tgccagttcg ttggagtaga tttcgtgaat
    11761 ttctccaatc gtcaaaggtt tgacttgaga gttcatcggt ttcttttgtg ctgccagctg
    11821 gaaacacagg cataatccca ttagcaaaat aagatttgat ttcatacgtg tttgtttatc
    11881 aaaaaaaatt aataaatatt tcattcaaga aggttgagca aatttcgata aaaataattg
    11941 aatacaaatt caagtaggta aataaaattt tgattcacga gcaatttgta aaaggagtct
    12001 attgattcaa ttcaaataca ggcattgctt attagcttaa tcataaaaaa acatccagca
    12061 gaaaaatctt actccagcca taaaatacga aaacgtactt actgagcatt aaattcgaaa
    12121 ttataattgg attgcaaaat atttagacag atatttgtct gtgatgctat tgcaaattta
    12181 accccgaatc tcatggaagt gaaacaatta atagacgaga atattttaac aacttccaaa
    12241 gaccagcccc aatctatcat aatggataca gacccttcga aaaagaaaat ggacgagtac
    12301 gaaaacttag gcaacgagct ggcgaggatt caggatattg cctccgtgat cagcgagcaa
    12361 aacaaacgtt tccaggtgat atgcagctca aaaaacgatg ctattattac ttcagacgag
    12421 agtaaaaaga tattattctg gaacaaaggt gccgaacaca tatttgggta tagtgcagag
    12481 gaagcaatcg gccaagctat gacaatcatt ataccacccg acctccaaca aagacataat
    12541 gaaggtatgg aaaggatgaa cttaagatag gcaccaaggg caataggcac agtactcgag
    12601 cttagggcct tccgaaaaaa cggacaggag tttccgatag aacttacgct cggtagttgg
    12661 gacaataacg gtaagaggta ttacagtggt atcatacgtg atattacaga aaagaaaaaa
    12721 gccgaagaaa ttatccttcg agaaaagaaa agatcggaag agctcattct gaatattttt
    12781 cccaaagaaa tagccgaaga attgaaagaa aatggaagaa cagaagcacg aaaacacgaa
    12841 aatgtatcca tcatctttac tgattttaaa gatttcactt cgctcacctc tacaatggca
    12901 cctaccatac tcatggagga gctcaacgaa atattcggat attttgatga tattttagaa
    12961 aaatacaaaa tagaaaaaat aaagaccatc ggtgatgcct actttgcagt ttgcggtctg
    13021 agtgaaggtg ttgaaaatca cgccttacaa tgtgtggaag ctacaaaaga gatgttcggg
    13081 tatctggcac tacgtaacaa aacaagccaa atcaagtggc gcatgcgagc aggcatacat
    13141 tcgggcccgg tagtagccgg tgtggtaagt aagaaaaaat acgcctacga cctttttggt
    13201 gatacagtca acacagctag caggatggaa tcaaacggaa aacccggaaa aataaacatt
    13261 tcggaagcaa cttttcaact aatcaaagag tggatcagtt gtaccccacg aggatctatc
    13321 agaaccaaag gaaaaggcgg tatgaatatg tattttgttg actaagaatt caacatgtta
    13381 agcactgtaa atacgattaa aatattaggc tcttgtcctg tggtgtcgcc atgccaatat
    13441 taacacaata agaaatggca atgtagataa aacaatctta aaattaaggc ttaacaatcc
    13501 aactacaaac ataaatacga gcaaaatgcc ggttcctgcc atgcctatat aactcaacac
    13561 tttacagggc tctttttgaa acaaacttac caacaacaat aattgaccca caaacggcaa
    13621 tattgttaac ggatggaata ccgaacccgg tgaagcaaat aacttggaaa ggaattcggc
    13681 ctccgcctcg tacaaaaaag aatgattgcc accgccccat tcgagataac cgaacaatga
    13741 tgtggtgatc aacagcagat taaggatttt ggatttcata cgatacttat tgttcaataa
    13801 atgattggtt gaaaaataaa ttttgtacaa taacaggaat tcattcttac agcgtatttt
    13861 caggtcttaa tagccattgc caggcatcac cgatatcatc atcttgtacg attcccgaat
    13921 atcggaatcc gttcttttca agaatttttg tagaagcgtt atgttcgggt gcggtgttgg
    13981 cagtaataat gataccgggt tggtgcattt tggcaatctt gattagttcc ttacatgcaa
    14041 atgatgctat accgcgacct tcatattcgc tgaatgtcca atatgcgatc tctactctac
    14101 cgtcattggg tggcccgaca aaactacacg aaccaactat ctgctcgtga tgcatgacaa
    14161 aatacccaac ccatggcagt ataaaaccgg tttttggata aaattggttg tacatgctta
    14221 gcagttgcct gcaatattcc gaatcgaaca taggatcaga tttgtcttca tcgggcgcta
    14281 ttggtttcaa tttttaagaa atttcttcca ttgttttgtt aaaatgttta ataaagctca
    14341 ggatatcaaa acccttgctg ttattataaa tttgctcccg ggctgccaaa ctgtttatac
    14401 aatgtcatca tttcggatta ttttgtaccg gcacccacac ttgctttaat cgcttctgct
    14461 acgtgcctgc cggtctcagc accaccattc ataaaaccct gaaacatagc actgcaatgt
    14521 tcacccgcaa acagaaactt tcctacaggc tgcatttcga gtccggcaat ggtagtccac
    14581 tgtccgggtt tataacatgc ataactaccc tttgtataag gaaaatcaat ccagttgaca
    14641 aaaatgtgct tgttattgaa gtgctttttt gaatttttga atgctttatc gaggacattc
    14701 acaaattcat caactttggc ctctggcagt tgggttcgcc aggtgtgttg tggcggcgac
    14761 attgggtttc ttacggctat tccatccaga tcgacagaga attttccgcc aaaataacaa
    14821 acaaatgcgg ctttgtcgga attgtcgagt ttgttgtagg agctatccca accattgacg
    14881 atgtctttat ggaacaggta gcccattgcc ttgttttcct ttgaacgcca gcttcgttca
    14941 ttataaccga gtaccagttt ggtattcatg ccataaccta gctcatttat acatttctgc
    15001 ttttcgggcg aaatattctt taagctcatc tttatctttc tgagtatggt aaaaggaata
    15061 gtacacacca ccatgcccgc taacaccggc ttctggtcgg tgaaagatat tttatagctg
    15121 ccgtcatcct gttcgcttac ctctgttact tcgtagcgct taagtatcct gtcgcttcct
    15181 atttttttct caagtccttc tattattttc gaattgccac ctttaatcct gaatctttcg
    15241 tcgctttcgc cgaatatttt aaacccacct gaagtatcag gatcgatgag atccaggaaa
    15301 tttatagtgg actgttcgtt gcagtcaaga ccaaattctg aaacaaatcc ggcaatcagc
    15361 acatccttca gccacttggc acatttgagt gaggcgaagt attctgacaa aggcattagg
    15421 tcaaattttt tggccgcctc ggtatcataa ttttcgccca acgacttgta gtcggcagct
    15481 attttgggag ctattttgcg aaactctcgt atgatgtctt tctctgtaaa aattttatca
    15541 ttgaacaaat aaacatcctt tactagtccg tctttcttca tttctgcttc aagatcgtat
    15601 aattcaagcc caaattcttt ggcaagggcc agcatgtctt catgcccggt atcgataaaa
    15661 tctccgccaa actccggata aatacctaaa cccagcggat cattgtaatg cgtaagtatt
    15721 cggccaccta cacgtttttg ggcttcgaat atcttaaaat catgacccga ctcaagcaaa
    15781 tggttagcac agttgagccc tgcaatgcct ccaccgagta tagcgatgtc gagtggtttc
    15841 tcaccgacaa ccggtggtgc atctgtatct gcagatgctg aagacggtgt gttgcggcag
    15901 gacgaaagaa atgcaggcat gctaacagac agcgatacac caatagctgc tttggccgaa
    15961 tgttcaataa atctgcggcg cgattgttgg atatttcttt tgttcctttc gggttggttt
    16021 tcaggcaatt tttcaagctc aaacttgagt aatgctacca gtttcctgaa catcctgctt
    16081 tttgggtgcg gcatagtgtt gtttattagt tctgatgtaa aacgctgtgt aaatttaaaa
    16141 aacaacagta aaaaatgcaa actagatgca gaatatttat ttatcaatac gcaatattat
    16201 taccacttgt attaataata ataagtctta tcgcatccga ttgctatttt catttggttt
    16261 acaaaacatc ccttttcata acggcaatat tcttatccgc taaggtcaat ctttgaactg
    16321 tattccagcc aagtctttgg tacaaatttt ctgctgtatg tgtaaacaaa taaataacac
    16381 tgcaaccaag atcacctgcc attttttcta tctccatgca gatggcactt ccggcaccgt
    16441 tgcctctgta ctcaggtatc gtgtaaacca aagccagcca atgtttgtgg attttcagat
    16501 gaggcagctt attgagcaac ccaacatggt tatataggcc accggttgct atgggttttc
    16561 caccaagtgt cagcaaaacc tgaaattctt cattgtctgt acccagccct tttatacgat
    16621 caatagtatc tgcttcagca atgccccatt catgaaaata ccaactcgca ataatcgaaa
    16681 tatacatttc atcatcgggc aatattttga gacatttata tttgtccatt cttgcttgtc
    16741 atgctttttt ctaaatgaat ctttacttta ttagctcctt ttgccaaaac catttcttaa
    16801 ctcgaagcta agcagaataa tgcttcctaa agataatagc ctggaaaata tacagtttac
    16861 cttattctac catagttgac tgaacatttc ttgatgcagc ttttattcgc tctatctggc
    16921 tcaggtgtct gatgatgtga ttaatataaa actgaaaggt atcgcccagt tttaatgata
    16981 tgagccgcga aatagaagtg ggcactttta ctttgttcag acttaccttg cctgatcgtt
    17041 ccaaaagttc gagaagtttg atctgttggg cacagaattt atctatcacc tgcttatcaa
    17101 gatttgcatg gagcggattc ttatctttaa aagtactcat cttattcatc ttttgcccag
    17161 gcaacatact tttggcaaaa tacgccccca gcagcccgct tctaaattct gcatccggtt
    17221 tggcaaccga tttgtttata ctgttatcta ttgccggcaa ataaaaatct ccgtacaaat
    17281 tcagatgttc gagacattcc aggatattcc acgaattttc agtttccctc cagctaagca
    17341 tgttgatgct ttcatctttc agcatttctg tctggctaat tatctgtttt gtttggtcga
    17401 tgagtttttc aattaaattt tcagactgca taatgattaa tttttataca aaggtttaac
    17461 tattattcgt cataaatatt gattgaaatc aagacttttt cagcctcgaa agtgtttcgg
    17521 ggctcattct caaataattg gctatgtatc tgttgggaat atattgaaac agatgcgggc
    17581 ttcgactcaa taccctttg
//
LOCUS       DAOQQY010000025        17072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1045_length_17072_cov_6.332080, whole genome shotgun sequence.
ACCESSION   DAOQQY010000025 DAOQQY010000000
VERSION     DAOQQY010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 17072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17072
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1045_length_17072_cov_6.332080"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="PLE23_02645"
     CDS             <1..771
                     /locus_tag="PLE23_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMX82194.1"
                     /translation="TTVNNAVNIGRSQGYSPDQPIKFSHATHAGLNKIDCQYCHDGAR
                     RSKHSLIPAANTCMNCHKAIKVGSKYGTGELTKIYASIGFNPNTDQYIANYDNMSEAD
                     IAKIYKQWIGDSYVKEKELPALDDNGKIEVERQWAELKSSLTNENKPKIQGPIEWIRI
                     HNLPDHVYFNHSQHVTVGKLECQKCHGKIQEMEVVKQYSSLSMGWCVNCHRETEVKFQ
                     DNAFYNSYKRYHDELKNGKREKVTVEDIGGIECQKCHY"
     gene            805..3990
                     /locus_tag="PLE23_02650"
     CDS             805..3990
                     /locus_tag="PLE23_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HMX82195.1"
                     /translation="MKNKEQNVWTGIEHLNPSPEFLENANQEFLHGQEEIPIPITEQS
                     LSLSSNRRDFLKYLGFGIGAATIAASCEIPVKRALPYVTKPDTIVPGVANYYASSFVK
                     GGDYCSILVKTREGRPIKIEGNSLSPVTKGGTNARTQASVLDLYDTNRYQTPKKVESG
                     KVKEVKWADLDRDVMAKLNSATGIRILSNTILSPTAKKAIGEFTTKYPNAKWVSYDPV
                     SASGLLDANLESFGDRVVPAYSFDKANVIVGFDCDFLGTWISPIEYAAQYATNRRVED
                     VKAAKMSRHYQVESHMSLTGSNADNRILVKPSEQGAAIAALYNAVASQLGSATISGPK
                     LNDKASKAMAGVAKDLIANKGASLVVSGSNNKYEQILVNKINDLLGNYGATISFNNAS
                     LQRTGDDAALQQLVSEMNAGSVGVLIVIDANPVYDLPNGEQFKQAMAKVGLKVSTAAV
                     PSETAVVCDYIAPAYHYLESWADAEPKRGSYSLIQPTISPLFDTRQFEASLLVWAASP
                     AYDTKAEQPYYEYLKSSWQSGMFGQQKAFSTFQSFWDSTLHDGILENPKSSNAGAFRG
                     EVADFGSKLTQPSSSELEISFYETINVGDGSYATNPWLQELPDPVTRTVWGNYLAVPV
                     GWKGEAKYDTFKGLKDGDLVDLEVNGKKLRVPVITQFGQMPGTLGLALGYGRTEASVT
                     GKNVGVNSFNMASVVNGATAYSSDKVKVSGKVGTEKDFSCVQYHHTMGVTGTDNKTKQ
                     KINVDELAAVTLGAGYQGSIVPRTIIRKAHVSEVSKFAETLHEERLGHQALNAKTLYR
                     KHDEKYKTGIHWGMHIDLNSCIGCGACSVACMSENNVPVVGKHEVHRHHEMSWLRIDR
                     YFYGTLENPNTVYQPMMCQHCDNAPCENVCPVAATNHSMEGINQMTYNRCIGTRYCAN
                     NCPYKVRRFNWLDYTTADLFPSNEYSINNEKVRFGADNLTRMVLNPDVTVRTRGVIEK
                     CSFCIQRVQEGKLTAKSENRQLRDGDVKSACMTACPTGAITFGNINDPESAVSKKNAN
                     SINYLVLEEVNVAPNVMYSAKIINSNESLDA"
     gene            4019..5410
                     /gene="nrfD"
                     /locus_tag="PLE23_02655"
     CDS             4019..5410
                     /gene="nrfD"
                     /locus_tag="PLE23_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HMX82196.1"
                     /translation="MGHYVSPLRIPLIEGNKTYHDITNDICAPTERTPSALWVLGFAI
                     SVAFLALYVFIITWTIWKGIGSWNLNRTIGWGWDITNFVWWVGIGHAGTLISAILLLF
                     RQRWRTGVNRAAEAMTIFAVICAGQFPIFHMGRVWLAFFVFPYPNTRGPLWLNFNSPL
                     MWDVFALSTYFTVSLLFWYTGLVPDFATVRDRAKGLRWKIYNFLSFGWTGSAKHWQRW
                     ESLSLILAGLSTPLVLSVHTIVSFDFATSVIPGWHTTIFPPYFVAGAIFSGFAMVLTL
                     MIIARKVLNLEQYITIEHIESMNKVILLTGSIVGVAYLTELFVAWYSGYIYEQFAFYN
                     RVLGPYWWSYVGMMTCNVISPQFFWSKKIRRSIAWTFGLSIVVNIGMWFERFVIIATT
                     LARDYLPSSWSYYTPSWVEVGMFLGSIGLFFTCFLIFTRVAPVVAIAEIKSILKVAGD
                     QYIGPKAKKHDNH"
     gene            5444..5971
                     /locus_tag="PLE23_02660"
     CDS             5444..5971
                     /locus_tag="PLE23_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HMX82197.1"
                     /translation="MKTLNKEVLFGLYNDETELLSAIKQANDKHLEIMDVFTPFPVHG
                     LDPLLGLKESRLHIAGFIYGATGTLIAFLFMTWVFVSDWPIIFGGKPFFAAPSFIPVT
                     FEFTVLLSAIGMTVTFYTICGMGPGVVNPILDSRITDNKFCIAFDVTHGKENENDYRS
                     FFTNTGASEVHSKNI"
     gene            5995..6828
                     /locus_tag="PLE23_02665"
     CDS             5995..6828
                     /locus_tag="PLE23_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMX82198.1"
                     /translation="MNTLKNFALLLVITLIFGACSGPSGNYPGTDYMPDMGYSVANEA
                     NVYNNYWANSWDEESVISRKATSMPRLPVKGTIPRGYAGYALSVNPDGTLALLNGESS
                     PNAIHTPINGDVPYYYADTEEDRNRAMKEITKNPFPITSAAIERGKNLYVIYCGICHG
                     DKGDGSGYLVRDNDGKYPAQPANLINDEFTAASEGRFYHAIMHGKNAMGSYADKLSYE
                     ERWDVIHFIRSLQAGAKGAKYDESENTLNSATPASKVVKKMAEVKVEAVAKPEDTNKK
                     H"
     gene            6865..8172
                     /locus_tag="PLE23_02670"
     CDS             6865..8172
                     /locus_tag="PLE23_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82199.1"
                     /translation="MNNQFVFEKKQKTTLGIFMLVGVVCMILSYLMDDGTLHMRFWSN
                     YLTNTVYFLGIAFIITFVLHAKVLMYSGWHVAFQRVWEAFSLFLIVGIVLMIPIIAGI
                     WGHFHHLYHWADKAAVASDVVLKGKSSFLNPVWYTAGTLIIVGVWYFFFAKRFRNLSV
                     QEDKEFDPNYTLHKKIKVNAGVFLPIAAFTSAALIWLWIMSVDAHWYSTLYAWYATAS
                     WWVGSLALTILTLIYLKSKGYLDIVTTEHLHDLGKYMFAFTIFWTYLWFSQYMLIWYA
                     NIGEETTYFHVRMRKFPVLFYGNLVLNFVLPFIILMRNDTKRKYGTLILTSIIVFVGH
                     WFDFFQMVRPGTWLSEIEHANHAAHATHASHAAEHAAPVLSKWGYPEGFVNGVSIPGL
                     LEIGILLGFVGLFFWFVFMQLEKASLVPKNNPYLEETEHHHVM"
     gene            8204..9700
                     /locus_tag="PLE23_02675"
     CDS             8204..9700
                     /locus_tag="PLE23_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82200.1"
                     /translation="MTAFIIFICLVLLAIVIIQVGKVTELASKIKGEEEAQYQSNFWN
                     SNLSLIFMVVFLGAIFVSAYYYKNSMLGYGPHIAASKHGKTIDFLFNVNLWFTGIVFV
                     LTHIALFWFAYKYSGKRNHRATFISHDNKLEIIWTAIPAAVMTLLVVGGLDAWNDIMA
                     DIKEKDEYIEIEATGMQFAWLLRYPGADGKLGTRDFKKISSNNPFGQDWTDTKNLDDI
                     HASEIVLPVGKKVRVRITSRDVLHSFFLPHFRVKMDAVPGMPTYFVFTPTTTTAEYRQ
                     RLSRYNEWQEPSDPKDPESKPRWQVFDYELACAELCGKGHFSMRRVVRIVSEDEYAQW
                     LKTQKSYYTESIKGTADDPNAGGQVSAEELKLRRETFLAELEKVKTQAADAGEKVVQL
                     GHIGFETGSAKLTTESKYQLDNLFEYLQSNADVKIELGGHTDSIGDAAVNLKLSGERA
                     KSVADYLISKGIDASRVSSKGYGSTKPVDSADTEEARAKNRRTEIKFL"
     gene            9721..11565
                     /locus_tag="PLE23_02680"
     CDS             9721..11565
                     /locus_tag="PLE23_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMX82201.1"
                     /translation="MSNISQIDQEKLIHEDGFNDHFHEHHDGDKYQMGFLTKYIFSMD
                     HKMIAKQFLITGMVWAVIGALMSLVFRLQLGFPESDMAWLRPILGQWIVVNDAGVGTL
                     STEFYYALVTMHGTILVFFVLTAGLSGTFSNLLIPLQIGARDMASPFLNMLSYWFFFL
                     AGIIMFISLFLSTGPFSGGWTAYPPLSLLPQASDGSKAGMTLWSISMVFFVVSVLLGG
                     INYVTTVLNLRTKGMSMWRLPLTVWSFLITAIVGILSFPVLAAAFFLVICDRSLGTSF
                     YLSEIFIGGQALDHVGGSAILYQHLFWFLGHPEVYIIILPTMGIVSEILSISSRKPIF
                     GYKAMVMSLSAISFLSFIVWAHHMFMSGMNPFITNFFVIFTLIIAVPSAVKVFNWIST
                     LYKGNIRFNSASLFAIGYVSLFISGGLTGIFLGNSTIDIQLHDTYFVVAHFHLVMGVA
                     AFFGMFAGIYHWFPKMFGRFMNETVGKIHFWGTLIGAYMIFWPQHYLGIAGVPRRYYS
                     FENFDAFSHFGTMNKVITIAAFFTFFLQVLFLINFFYSMYRGRKVTNPNPWGANTLEW
                     TTPINPGHGNWDGNLPVVHRWPYDYSKDGEDHIPQIIPIQPGETVVHQ"
     gene            11598..12494
                     /gene="cyoE"
                     /locus_tag="PLE23_02685"
     CDS             11598..12494
                     /gene="cyoE"
                     /locus_tag="PLE23_02685"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HMX82202.1"
                     /translation="MLKSLGLIDTIRLKLTDLGLLTKFRLSSLVVLSAMAGYFVSLHT
                     AVTLEGLLTIAIGGFLVTGAANALNQVFEIEYDRQMKRTQNRPLAAGRMYIPEAVLWA
                     GVFSLTGTALLLSFNLYAGILGLTSLVLYAFIYTPLKRISPVAVWVGALPGALPVLIG
                     GVAAAGYLTPLAICLFLLQVIWQFSHFWAIAWVADDDYRNAGFKLLPSNSGEKDSSVG
                     FQAFVYNIALTLLGILPYFLGLMSLISTIIIVLVSILYSQKAWYLYRRCDMDSARKLM
                     FSSFLYLPVVLLVFVIEKMLMI"
     gene            12491..13063
                     /locus_tag="PLE23_02690"
     CDS             12491..13063
                     /locus_tag="PLE23_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMX82203.1"
                     /translation="MSSTVVVQKSKIDPKFFTLLIACASIFMMFAGLTSAYVVRQAGG
                     NWLEFTLPDSFTYSTIIIIISSVFLHFSYFSINKGKEASYKIFLVVAFIMGLFFLVLQ
                     YYGWTQMENMGIMFNGNPSGSFVYVISAVHAAHVLGGIGALAMAMIHALSLDFFVNDK
                     RRNRFKITVTYWHFVDILWIYLFLFFNVQR"
     gene            13078..13941
                     /locus_tag="PLE23_02695"
     CDS             13078..13941
                     /locus_tag="PLE23_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMX82204.1"
                     /translation="MANDTVLNQNGEQDVWSGAKEPFRASYGKLMMWYFLLSDAFTFA
                     GFLVAYGALRFSVPTWPLPDFVFSTFPGGIHHAPLIFVTVMTFILIMSSVTMVRAVQE
                     GHRENKKGVVFWMLMTIVGGLSFLGCQAWEWTNLIGKENMTIYQNPFGTHTQDGVYLD
                     NEHGVPYAAGEKAETFKAHHTYLIEKVANHDTGKKDWVQRRMKLKDGTETVQQFGPQA
                     FGALFFFITGFHGFHVFSGVVFLTIIMLNVASGLYAQRKNHYEMVEKIGLYWHFVDLV
                     WVFVFLVFYLL"
     gene            13963..14358
                     /locus_tag="PLE23_02700"
     CDS             13963..14358
                     /locus_tag="PLE23_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HMX82205.1"
                     /translation="MAELDIEAGKKVVIKGLILLGVVTLVEVFIALFGNGHVIDGFHL
                     PKWLMYPMMIGLSLYKAYFIIFEFMHMKYEVKGLRASVLLPTILLIWAMLALLNEGSA
                     WGNSNAKINKWNTAEPGKKATAPPTKEMK"
     gene            14449..15039
                     /locus_tag="PLE23_02705"
     CDS             14449..15039
                     /locus_tag="PLE23_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82206.1"
                     /translation="MTRKKGIFQMFLLFMMLIGFPAVSWYYLNGGLDYRVNAKNELKN
                     YGNIRQLSLTSLDDKQVFTDSLSVYLLQTNHSPLLDSVFKVILQAYDARNDIQALDCY
                     CNGQSRTDTSAVINMIRLKNDINICDFLKNTVDAIKTKSNVNADVILVDTKGNVVQGY
                     NLGVSDDRARLIKHVSILMPNVVRSDKPVKSIIREK"
     gene            15169..15423
                     /locus_tag="PLE23_02710"
     CDS             15169..15423
                     /locus_tag="PLE23_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82207.1"
                     /translation="MKKYLRFFFVFLLVLTVFTLVDAQCPMCSMTAETNMVGGGRAGR
                     GLNSGIIYLLMAPYVLVGGIAFYWFRYRRKDDAVDPVNND"
     gene            complement(15505..16185)
                     /locus_tag="PLE23_02715"
     CDS             complement(15505..16185)
                     /locus_tag="PLE23_02715"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMX82208.1"
                     /translation="MKTKIIILAATMFASLFVNAQISVGVKTGINFADASLSGISETL
                     LPDAKIINTPLAGVNLQYRINEQFSFQPELNYAQRGFEAKAGTAFNVLDFNIPVGIKA
                     ITKVNYIELPVQLKYKFTTGNVQPYIMAGPSISYATSGTIREVASAIIDINLGEQDIN
                     LSNKNFNQWEAGGRLSAGVAFKTLLGEIQLEGSYYHGLTDFFTNPIVDVHAYNKGFSM
                     QLGWAYHF"
     gene            complement(16364..16735)
                     /locus_tag="PLE23_02720"
     CDS             complement(16364..16735)
                     /locus_tag="PLE23_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82209.1"
                     /translation="MKQILFIISIVLLAHSYANAVVVVKTDTLSLTAKNGTSYNHTNV
                     VKKNSIKTIVKKIRHFFTFDLSDPVDGWLSWAILCFAGAIGVGLVSWLLGGIYLLGFI
                     SSLLAIAGLVFFVVYIIKLVG"
BASE COUNT         5014 a         3062 c         3650 g         5346 t
ORIGIN      
        1 acaaccgtta ataacgccgt taatatagga cgttctcagg gctattcacc tgaccaaccg
       61 ataaaattct cacacgcaac acatgccgga ctcaacaaaa tagattgcca gtattgccat
      121 gatggtgcta gaaggagtaa acattcactc attccggctg ccaatacttg tatgaattgc
      181 cacaaggcaa tcaaggtcgg ctctaaatat ggaaccggtg agcttacaaa aatctacgct
      241 tccataggct ttaaccctaa tactgaccaa tatatcgcta actacgataa tatgtctgaa
      301 gcagatatag caaaaatata caaacagtgg atcggtgact cttatgtaaa ggagaaagaa
      361 ctaccggcac ttgatgataa tggaaagata gaagtagaaa gacaatgggc tgaattaaag
      421 tcgtctctta ccaacgagaa caaaccaaaa atacaaggcc caatagaatg gattagaatt
      481 cataatcttc ctgatcatgt atattttaac cactcacagc acgttacagt aggcaagctt
      541 gaatgccaga agtgccacgg taaaatccag gaaatggaag tagtgaaaca atattcttcg
      601 ctctcaatgg gctggtgtgt taattgtcac cgtgaaacag aagtgaagtt ccaagacaat
      661 gcattttata attcctacaa aagataccac gatgagttga agaacggcaa acgcgagaag
      721 gtaacagtag aagatatcgg cggtatcgaa tgtcaaaaat gccattatta atcagaaact
      781 taagagcgca taatatttaa ctctatgaaa aacaaagagc aaaacgtttg gacaggaatt
      841 gaacacctaa atccctctcc ggaatttctt gaaaatgcta atcaggaatt tttgcacggc
      901 caggaagaaa ttcctattcc aataactgag caatcattaa gtttgtcttc taacagaaga
      961 gactttttaa aatatttggg tttcggaatt ggcgctgcta ccattgcagc aagttgtgaa
     1021 atcccggtca aaagagcact tccttatgta accaaacctg ataccattgt tccgggtgtg
     1081 gccaattatt acgcatcttc ctttgtaaaa ggtggggatt attgctccat tcttgtgaaa
     1141 acgagagaag gacgtcctat taaaattgaa ggtaattcgc tttctccggt taccaagggt
     1201 ggaaccaacg ccagaactca agctagtgta ctcgaccttt atgatacaaa tagatatcag
     1261 actccaaaaa aagtggagtc cggaaaagtg aaggaagtaa aatgggcaga cctcgataga
     1321 gatgtaatgg ccaagctcaa ttctgccacc ggtatcagaa tcttatctaa tacaatacta
     1381 agcccgactg ccaagaaagc aatcggagag tttactacta agtatcctaa tgcaaaatgg
     1441 gtaagctatg acccagtttc ggcaagtgga ctgttggatg ccaatcttga atctttcggt
     1501 gacagagtgg tacctgctta cagttttgat aaggcaaatg taattgtggg ctttgattgt
     1561 gatttcctcg gcacttggat ttcgcctatt gaatacgctg cacaatacgc tacgaataga
     1621 agagtagaag atgtaaaggc cgccaagatg tcaagacact atcaggtgga aagtcacatg
     1681 tcactcacag gctcaaatgc agataaccgt attctggtaa aaccatctga acagggtgct
     1741 gcaattgctg ctttgtataa cgcagtagcg tcacaattgg gtagtgcaac tatctccggc
     1801 ccgaaactga atgataaagc atctaaagcg atggcaggtg ttgcaaaaga tttgattgct
     1861 aataaaggtg cttcactggt tgtttccggt tcgaataata agtatgaaca aatacttgtt
     1921 aataaaatta atgacctcct cggaaattat ggtgctacca ttagttttaa caacgcttca
     1981 ctacaacgca ccggagatga tgccgcctta cagcaattag ttagtgaaat gaatgccgga
     2041 tcagtaggcg tacttattgt tattgacgct aatcctgttt atgatttgcc gaatggcgaa
     2101 caatttaagc aggctatggc taaagtgggc ttaaaagttt ctactgcagc agtaccttct
     2161 gagacagcag tggtgtgcga ttatatcgct cctgcctatc actacctcga atcatgggca
     2221 gacgctgaac caaagagagg aagttatagc ttgatacagc caactatttc tccacttttt
     2281 gatacaagac aattcgaggc ttcgctcttg gtgtgggcag caagccctgc ttatgatacc
     2341 aaggcagaac agccgtatta tgaatacctt aagagcagct ggcagtcagg tatgttcggt
     2401 cagcaaaaag ctttcagtac attccagtct ttctgggata gtacattgca tgatggtatt
     2461 ttagaaaatc caaaatcatc caatgctggt gctttccgtg gcgaagttgc tgattttggt
     2521 tcaaaactta ctcaaccatc ttcttccgaa cttgaaattt cattctacga aaccatcaat
     2581 gtaggtgacg gatcgtatgc taccaatccg tggttgcagg aattaccgga tccggtaaca
     2641 agaacagttt ggggtaacta cctcgctgta ccggtagggt ggaaaggaga agcaaaatac
     2701 gacactttca aaggccttaa agatggtgac cttgttgact tggaagtaaa tggcaaaaaa
     2761 cttcgggtac ctgtcattac acaatttggt caaatgccgg gcaccttagg tcttgccttg
     2821 ggatatggtc gtactgaagc cagcgtcaca ggtaaaaatg tgggtgttaa ttcatttaat
     2881 atggcatccg ttgtgaatgg tgccacggct tatagctctg ataaagtaaa agtttctggc
     2941 aaagttggta ccgagaaaga tttttcttgt gtacagtatc accatacaat gggtgtaacg
     3001 ggaactgaca ataaaaccaa acaaaagatt aatgttgacg aacttgctgc tgttacatta
     3061 ggtgcaggtt atcagggttc gatcgtgcca agaaccatta tcagaaaggc acatgtcagt
     3121 gaagttagta aatttgctga aacactccac gaagagagat tgggtcatca ggctctaaat
     3181 gccaagacac tctatagaaa gcatgatgaa aagtacaaaa cgggtatcca ttggggtatg
     3241 cacatcgacc tcaattcttg catcggttgt ggtgcttgtt cggtagcttg tatgtcggag
     3301 aataatgtac ctgttgtagg taagcatgaa gtacaccgac atcatgaaat gagctggtta
     3361 agaatcgaca gatatttcta cggcacactc gaaaacccga atactgttta ccaaccaatg
     3421 atgtgccagc attgtgacaa cgctccttgc gagaatgtct gcccggtagc agccaccaac
     3481 catagcatgg aaggtattaa tcagatgact tacaacagat gtatcggtac aagatattgt
     3541 gccaataact gtccgtataa agtgagaagg tttaactggc tagattatac aacagccgat
     3601 ttattccctt ctaatgaata ttctatcaac aatgaaaaag tcagattcgg tgcagacaac
     3661 cttaccagaa tggtattgaa tccggatgtg actgtgcgta cacgtggtgt aattgaaaaa
     3721 tgtagcttct gtattcaaag agttcaggaa ggtaaactta ctgctaagag tgaaaacaga
     3781 caactgcgcg atggtgatgt aaagtctgca tgtatgactg cctgtcctac aggtgctatt
     3841 actttcggaa acatcaatga tcctgaaagt gcggttagta aaaagaatgc aaattctata
     3901 aactaccttg tacttgaaga agttaacgtt gcaccaaatg ttatgtacag cgcaaaaatt
     3961 attaacagta acgaaagtct tgacgcttaa tttataataa cgaatattaa atatagatat
     4021 gggacactat gtttctccgc taaggatacc actcattgaa ggtaacaaaa cgtatcacga
     4081 tatcaccaat gacatttgtg cacctaccga gcgtacacca agtgcattat gggtattggg
     4141 tttcgctatt tcagtagcgt ttttggcact ttatgttttt atcatcacct ggacaatctg
     4201 gaaaggtatc ggatcatgga accttaacag aaccatcgga tggggttggg atataaccaa
     4261 ctttgtttgg tgggtcggta taggtcacgc tggtacactt atttctgcaa tccttttgct
     4321 tttcaggcaa agatggcgta caggcgttaa cagagccgct gaagcgatga cgatttttgc
     4381 cgttatctgt gccggacagt ttccgatatt tcacatgggt agagtatggc ttgcattctt
     4441 tgtattcccg tacccaaata ccagaggtcc gctgtggctt aactttaaca gcccgctgat
     4501 gtgggacgtt ttcgcccttt ctacatattt tacagtctca ttactattct ggtacaccgg
     4561 acttgtacct gattttgcta cagtaaggga ccgagctaaa ggtttgagat ggaaaatcta
     4621 taatttttta tctttcggat ggaccggttc tgccaagcat tggcaaagat gggagtcact
     4681 ttctcttatt ctggcgggcc tttctactcc acttgtactt tcagtgcaca ccattgtatc
     4741 atttgacttt gccacttcag taataccagg ttggcacacc accattttcc ctccttattt
     4801 tgtagcaggt gctatcttct caggcttcgc aatggtattg actctgatga ttattgcacg
     4861 taaagtgctc aatcttgaac agtacataac catcgagcat attgagtcga tgaataaagt
     4921 aattctcctg actggttcaa ttgtaggtgt tgcatatctt acagaacttt ttgtggcttg
     4981 gtattcagga tatatatacg agcagtttgc tttctacaac agggttttgg gtccatattg
     5041 gtggtcctat gttggtatga tgacttgtaa cgttatttct ccacagtttt tttggtcaaa
     5101 gaagatcaga agaagtattg cctggacatt cggtctatcg attgtagtaa atatcgggat
     5161 gtggttcgaa cgttttgtta tcatcgccac gacacttgcc agagattacc ttccatccag
     5221 ctggagttac tatactcctt catgggtaga ggttggtatg ttcttgggct caattggttt
     5281 attctttacc tgcttcctta tctttacaag ggtagcacca gtggtagcaa ttgctgagat
     5341 taagtcgatc ctgaaagttg caggtgatca atacattggg ccaaaggcta aaaaacacga
     5401 taatcattaa ttagtttgac tgaaaagatt atatcaaaag tatatgaaaa cattaaataa
     5461 agaagttctt tttggtttgt acaatgatga aaccgaattg ctgagcgcca tcaaacaggc
     5521 gaatgataag caccttgaaa tcatggatgt ttttacacct tttccggtgc acggtctcga
     5581 ccctttgctt ggtttgaaag aatcaagatt gcacatcgca ggttttattt atggtgctac
     5641 cggtaccttg attgcatttc tgtttatgac ttgggtgttc gtcagcgact ggccaattat
     5701 ctttggtggt aaaccattct ttgcagctcc ttcttttatt ccggtaactt ttgaatttac
     5761 tgtactcttg tctgcaatag gcatgacagt aacattttat acaatatgtg gcatggggcc
     5821 cggtgtcgtc aatccgatac tggacagcag gataacagat aataaattct gtattgcatt
     5881 tgatgtaaca catggaaagg aaaatgaaaa tgattataga tcatttttta ctaacactgg
     5941 tgcatcagaa gttcattcaa aaaatattta ataaaagcat aaacatcctt caagatgaat
     6001 actttgaaaa attttgcttt gcttctagta ataaccctga tatttggtgc ctgctcgggt
     6061 ccaagtggta attatccggg aacggattat atgcctgata tgggttactc ggtagccaat
     6121 gaagctaatg tttataataa ttattgggct aacagctggg atgaagaaag tgtaatcagc
     6181 aggaaggcga cttccatgcc aagactgccg gtcaaaggta caatcccgcg tggttatgca
     6241 ggttatgcct tgtcggttaa tcctgatgga acacttgcat tacttaatgg cgaatcttca
     6301 ccaaatgcta tacacactcc tatcaatgga gacgtacctt attattatgc agatactgag
     6361 gaagacagaa acagagcaat gaaggaaatc actaagaatc ctttccctat tacaagtgcg
     6421 gcaattgaaa gagggaaaaa tctttacgtt atttattgtg gcatctgcca cggtgataaa
     6481 ggtgatggta gtggttatct tgttagagac aatgacggaa agtatccggc tcagcctgct
     6541 aaccttatta atgatgagtt tacagctgcc agcgagggta gattttacca tgctatcatg
     6601 catggaaaaa atgcaatggg tagttatgcc gataaattgt cgtatgaaga aagatgggat
     6661 gttatccact ttatccgaag tttacaggca ggtgccaagg gggctaagta tgatgaaagt
     6721 gaaaatacac tcaacagcgc taccccagct tctaaagttg ttaaaaaaat ggcagaagtg
     6781 aaagttgagg cggttgcaaa accagaggac acaaacaaaa aacactaaga aagttattaa
     6841 caagctataa aagaattttt tcttatgaat aaccagtttg tatttgaaaa gaaacagaaa
     6901 actactttgg gcatctttat gctggtagga gtggtctgta tgatattgag ctatttaatg
     6961 gatgacggga ctttacacat gaggttttgg tcaaattacc taacaaatac ggtatatttt
     7021 ctcggcattg catttataat aactttcgta ctacatgcca aagtactaat gtattccggc
     7081 tggcatgtgg cattccaacg agtttgggaa gcattttctt tgttccttat cgtcggaata
     7141 gtattaatga ttcctataat agcaggtatc tgggggcatt ttcaccatct ataccattgg
     7201 gcggataaag ctgctgttgc ttccgatgtt gtccttaaag gtaagtcttc attccttaac
     7261 cctgtatggt acactgccgg tactttaatt atagtcggcg tatggtactt ctttttcgca
     7321 aagagattta gaaacctatc agtacaggaa gataaagagt ttgatcctaa ctatacactt
     7381 cataagaaaa ttaaagtcaa tgcaggtgtt ttcctaccga tagctgcatt tactagtgcc
     7441 gctttgattt ggctttggat aatgtctgtt gacgcacact ggtactcaac tttgtatgcc
     7501 tggtatgcta ctgcaagttg gtgggttggt tctctggctt tgactatcct aacactcatt
     7561 tatcttaaga gcaaaggata tcttgatatt gttactacag aacacctcca tgatcttggt
     7621 aagtacatgt tcgcctttac gattttctgg acttacctct ggttttcaca atatatgttg
     7681 atttggtacg caaatattgg tgaagagacg acatattttc acgtaaggat gagaaaattc
     7741 ccggtattgt tctatggcaa tcttgtactt aatttcgtac tgcctttcat catcctcatg
     7801 aggaatgaca caaagagaaa atacggtaca cttattttga catctattat tgtttttgta
     7861 ggtcactggt ttgatttttt ccagatggta agacctggta cttggttgtc agaaattgaa
     7921 catgcaaatc atgctgcaca tgcaacacac gcaagccatg ctgcagagca tgcagcacct
     7981 gtgttgagca aatggggata tcccgaagga tttgtaaatg gcgtcagtat tcctggatta
     8041 ttggaaatag gaattttatt aggcttcgta ggcttgttct tctggtttgt atttatgcag
     8101 cttgaaaaag catcactcgt tccaaagaat aatccttacc ttgaggaaac tgaacatcac
     8161 cacgtaatgt aataaattaa aataaagaga taaatatata tttatgacag cctttataat
     8221 ttttatctgc ctcgtactgc tggctattgt aataatacaa gttggtaagg taacggagct
     8281 tgccagtaag attaaaggag aagaggaagc tcaataccag tcaaatttct ggaactctaa
     8341 cctatctttg atttttatgg ttgtttttct cggagcaatt tttgtttctg cttattacta
     8401 taagaattcg atgttgggtt atgggcctca tattgccgcc tcaaagcatg gtaaaaccat
     8461 tgactttttg tttaacgtaa atttgtggtt taccggcatt gtgttcgtcc tgacacatat
     8521 tgcattattt tggtttgcct ataaatatag cggtaagagg aaccacagag ctactttcat
     8581 ttctcatgat aataaattgg aaatcatctg gactgcaatt ccagctgcag taatgacctt
     8641 acttgtagta ggaggattgg atgcatggaa tgatatcatg gccgatatta aggaaaagga
     8701 cgaatatatt gagattgaag ctaccgggat gcagtttgcc tggctattaa gatacccagg
     8761 tgctgatgga aaactaggta ctagagattt taagaaaatt tcaagtaata acccatttgg
     8821 acaggattgg acagatacaa aaaatcttga tgacattcat gcctctgaga tagtgttgcc
     8881 ggttggtaaa aaagtacgtg tcaggattac ttcacgcgat gtgcttcata gttttttcct
     8941 accacatttc cgtgtaaaaa tggatgctgt gcctggtatg cctacatatt ttgtatttac
     9001 accaacaacc acaacagccg aatatcggca acgcttgagc agatacaatg aatggcagga
     9061 accatctgat ccgaaagatc cggaaagtaa accaagatgg caggtatttg actacgaatt
     9121 agcttgtgca gaattgtgtg gtaaaggtca ttttagtatg aggcgagttg ttagaattgt
     9181 atcagaagat gaatatgcac aatggttaaa aacacaaaaa tcttattata ccgagtcaat
     9241 taaaggtaca gctgatgacc ccaatgctgg tggacaagta tcagctgaag aattgaaatt
     9301 aagacgtgaa acatttttgg ctgagttaga aaaagtgaaa acgcaagctg cagatgcagg
     9361 tgagaaagtt gtacaattgg gccacattgg ttttgaaaca gggtccgcaa aacttactac
     9421 agaatcaaaa tatcagcttg ataatctttt cgaatattta cagtctaatg ctgatgttaa
     9481 aatagaactt ggaggacata cagacagtat aggcgatgca gctgttaatc taaaactttc
     9541 aggtgaacgg gcgaaaagtg ttgctgatta ccttattagt aaaggtatag atgcttctcg
     9601 cgtcagctct aaaggttatg gttctaccaa gccggtggat tctgctgata ctgaagaagc
     9661 tagagcaaag aaccgccgaa cagaaattaa atttttataa tatattgaaa ttttagtacc
     9721 atgtcaaata tttcacaaat agatcaggaa aaattgattc atgaagacgg ttttaacgac
     9781 cattttcacg aacatcatga tggtgataaa tatcaaatgg gattccttac gaagtatatc
     9841 ttcagtatgg atcataaaat gattgctaaa caattcctaa taacaggtat ggtatgggca
     9901 gtcataggag cattgatgtc tttggtgttc agactccagc taggctttcc agagtccgat
     9961 atggcttggc taagacctat tcttggtcaa tggattgttg tcaatgacgc tggcgtaggt
    10021 acattgtcca ctgagtttta ttatgctctg gtgacaatgc acggaactat tctggtgttt
    10081 tttgtattaa cagctggtct gagtggtaca ttcagtaact tgcttattcc gttgcaaatc
    10141 ggtgctcgtg acatggcttc acctttcctg aatatgttgt cgtactggtt tttcttcctg
    10201 gctggtatca tcatgttcat atcattattt ttgagcactg gtccattttc tggcggttgg
    10261 acagcatacc caccactgag tttgttgcct caggcatctg atggttcaaa agcgggtatg
    10321 actttatggt cgattagtat ggttttcttt gttgtatcag ttttgttggg tggtattaac
    10381 tatgttacca ctgtactcaa tctgcgtaca aagggaatgt ccatgtggag acttccgctt
    10441 acggtttggt ctttccttat tacagctata gttggtattt tgtctttccc tgtattagca
    10501 gctgcattct ttttggtcat ttgtgacaga agtcttggca cgagctttta tcttagtgaa
    10561 atatttattg gtggacaggc gcttgatcat gttggtggtt ctgcaatttt gtaccagcac
    10621 ttgttttggt tcttaggcca cccggaggta tatatcatta tattgcctac gatgggtatt
    10681 gtatctgaga tattatcgat tagctcaagg aaaccaattt ttggttataa agcgatggta
    10741 atgtcattga gtgcgatatc gttcctttcg tttattgtgt gggcgcacca catgttcatg
    10801 tcaggtatga atccatttat taccaatttc tttgttatat ttacacttat tattgcagtg
    10861 ccttcagcag tgaaggtatt taactggatt tccactttgt ataaaggtaa tattcggttc
    10921 aattctgcaa gtctttttgc aatcggttac gtttcattgt ttatatcagg tggtctgacg
    10981 ggaatttttc ttggtaactc cactattgat attcaattgc acgatactta tttcgtagta
    11041 gcgcacttcc accttgtaat gggtgtggct gcattctttg gtatgtttgc tggtatttat
    11101 cattggttcc ctaaaatgtt cggaaggttt atgaacgaaa ctgttggtaa aatacatttt
    11161 tggggcacgt tgattggagc ttacatgata ttctggcctc aacattacct aggtattgcc
    11221 ggtgtaccga gaagatatta cagctttgaa aacttcgatg cgttctctca ttttggtacc
    11281 atgaataagg tgattaccat agccgcattc tttacttttt tccttcaggt attgtttttg
    11341 attaacttct tctacagtat gtacagaggt agaaaagtaa ccaatccgaa tccttggggt
    11401 gcgaatacgc ttgaatggac aactccaata aaccctggac acggtaactg ggatggtaat
    11461 ctaccggtag tacacagatg gccttatgac tatagcaaag atggtgaaga ccatattcca
    11521 cagattattc cgattcaacc cggagaaact gttgttcatc agtaaatttt aattgagcga
    11581 tagcaattag aagagaaatg cttaaatcat taggactaat agataccata cggctcaaac
    11641 ttacagatct tgggctactg actaagttcc gcttgtcgtc attggttgtg ttatcagcca
    11701 tggccgggta ttttgtgtct cttcacacag ctgtgacact tgaaggtttg ttgaccattg
    11761 ctattggtgg ttttctggtt acaggtgcgg ccaatgcatt gaatcaagta tttgaaatag
    11821 agtacgacag acaaatgaaa cgtacacaaa accgacctct ggctgctggt agaatgtata
    11881 tacctgaggc tgtattgtgg gctggtgtat ttagtttgac aggtacagct ctgttgttaa
    11941 gttttaatct ttatgcagga atattgggac ttaccagctt ggttttgtat gctttcattt
    12001 atacgccgtt gaagaggatt tcacctgtag cggtgtgggt tggtgccctt cctggtgcct
    12061 tacctgtact gataggaggt gtggctgccg ccggttatct gacaccattg gccatttgtc
    12121 tttttttgtt acaagttatc tggcagtttt cacacttttg ggcaatcgca tgggttgcag
    12181 acgatgatta tcgaaatgct ggttttaaat tacttccaag taattctgga gagaaagatt
    12241 cgtcagtagg gttccaagct tttgtgtata atattgcact tactctgttg ggtatcttac
    12301 cttatttcct tggtttgatg agtcttatat ctacaattat tattgttttg gtatcaatat
    12361 tatactctca aaaagcatgg tatttgtata gaagatgcga tatggattct gcaagaaagc
    12421 ttatgttttc ttcattcctg tatctgcctg tagtcttgct tgtatttgtc atcgaaaaaa
    12481 tgttaatgat atgagttcaa cggtggtagt acagaaaagt aagattgatc caaaattctt
    12541 cacgctactt atagcttgtg ctagtatttt tatgatgttc gcaggcttga caagtgctta
    12601 tgtagtaagg caagcaggtg gcaactggtt ggaatttaca ttaccggatt cattcactta
    12661 cagtacaatt attattatta tcagcagtgt ttttctgcat ttttcatatt tttccatcaa
    12721 caaggggaaa gaagcttcgt acaagatatt tttagttgtt gccttcataa tgggtctttt
    12781 ctttttagta ctccaatatt atggatggac acagatggaa aacatgggta tcatgttcaa
    12841 tggaaatcct agtggttcgt ttgtgtatgt gataagcgca gtacacgcgg ctcacgtttt
    12901 gggtggcata ggtgcacttg caatggctat gatacatgca cttagtcttg atttctttgt
    12961 gaatgataaa cgcagaaacc gtttcaaaat aacagttact tactggcatt ttgttgatat
    13021 actatggatt tatttatttt tattctttaa tgttcaacgt taataaacac tagacgaatg
    13081 gcgaatgata ctgtattaaa tcaaaatggc gaacaagatg tttggtctgg tgccaaagaa
    13141 ccatttagag cgagttatgg aaagttaatg atgtggtatt tcctgctgtc tgatgctttt
    13201 actttcgcag gcttccttgt tgcttatggt gctcttagat tcagcgtacc tacttggcct
    13261 ttgcccgatt ttgtgttcag cactttccct ggtggcatcc accatgcacc actgatattt
    13321 gtgactgtta tgactttcat cttgataatg agttcggtca caatggtaag agccgtacag
    13381 gaagggcatc gcgaaaacaa gaaaggtgta gtattttgga tgctcatgac tatagtcgga
    13441 ggtctttcat ttcttgggtg ccaggcatgg gaatggacaa acttgattgg taaggaaaat
    13501 atgactatct accagaatcc gttcggtaca catactcaag atggtgtcta tcttgataat
    13561 gaacatggcg tgccttatgc agcaggtgaa aaagccgaga catttaaagc acatcatact
    13621 tatcttatcg aaaaggtagc caaccatgat acaggcaaaa aggattgggt acaacgaaga
    13681 atgaaactca aagatggtac tgaaacagtt cagcaatttg gtccgcaggc tttcggtgca
    13741 ttgtttttct tcattaccgg ttttcacggg tttcacgtat tttctggtgt agtgttcctt
    13801 accatcatca tgcttaatgt ggctagtggc ttgtacgcac aaagaaagaa tcactacgaa
    13861 atggtggaaa aaatcggtct atactggcac tttgtggatc tggtgtgggt atttgtattt
    13921 cttgtatttt atttactata ataattataa aaacagttaa tcatggcaga attagatata
    13981 gaagcaggta aaaaggtagt aataaaaggt cttatactat taggtgttgt taccttggtt
    14041 gaggttttta tagcgttgtt tggcaacggg cacgtcatag atggatttca tttaccgaag
    14101 tggctcatgt atccgatgat gattggactt agtctgtata aggcatattt tattattttt
    14161 gaattcatgc acatgaagta tgaagttaaa ggtcttagag ctagtgtgct tttacctact
    14221 attctgctga tatgggctat gcttgctctg ctcaatgaag gttcagcctg gggcaatagt
    14281 aatgcgaaaa taaataagtg gaatactgct gagcctggca agaaagctac agctccccca
    14341 actaaagaga tgaagtagtc ttagcgacaa tagaaaaggc ggataacaaa attatccgcc
    14401 tttttcattt acctttgcat ggctttaata tttttcaatt tttcgaaaat gaccagaaaa
    14461 aaaggtatat tccaaatgtt tttgctcttc atgatgctga taggttttcc ggcagtatca
    14521 tggtattatc tcaatggtgg ccttgactac agggttaatg ccaagaatga actaaagaat
    14581 tatggcaaca ttcggcaact cagtttgaca agtttggatg ataagcaggt ttttactgac
    14641 agccttagtg tttatctttt acaaacaaat cattctccgt tattggattc cgttttcaaa
    14701 gtaatacttc aggcttatga tgcacgcaat gatatccagg ctttagattg ctattgcaat
    14761 ggccaatcca gaaccgatac ttcagctgtc atcaatatga tcagattgaa gaatgatata
    14821 aacatttgcg atttcttaaa gaatactgtc gatgctatca agacaaaatc gaatgtgaat
    14881 gcagatgtca ttcttgttga tacgaagggc aatgtagtac aaggttataa tctcggtgta
    14941 agtgatgacc gagcgcgact aataaaacac gtttcgattc tcatgcctaa tgttgtacgg
    15001 tcagataaac ctgtcaaatc tattattcgc gaaaagtaat atataggagt taacgctcgt
    15061 tgttaaatga tttacttttc gtaagacgtt aaatattgca atctattgta ggatttgttg
    15121 gttttggttt tgttttgatg taattttgat ttccatttca attgaatcat gaaaaagtat
    15181 ttaagatttt ttttcgtatt cctgcttgtt ctcactgtat ttactttggt agatgcgcag
    15241 tgcccaatgt gcagtatgac tgcagaaacc aatatggtag gtggtggtag ggcaggtcga
    15301 gggctgaact ccggaataat ttatttactg atggcacctt atgtactcgt aggtggtata
    15361 gctttttatt ggttcaggta cagaagaaaa gatgatgctg ttgacccggt taacaatgat
    15421 tgaatttttt atttgtttag agtattaatt ttagggccgg aacgtgattt tccggcccat
    15481 cctatatata gagtgtgaat tactttagaa gtggtatgcc caaccaagct gcatgctgaa
    15541 acctttattg taagcatgta catcgacaat cgggtttgta aagaagtctg ttaacccgtg
    15601 atagtagcta ccttcaagtt gtatttcacc aagaagagtt ttgaaggcaa caccggcact
    15661 tagccttcca cctgcttccc attggttaaa atttttgttg ctaagattga tgtcctgctc
    15721 acccaagtta atatctataa tggctgatgc cacctccctt atggtcccgg atgtagcata
    15781 cgatattgac ggacctgcca ttatatatgg ttgcacattg ccggtagtga atttatattt
    15841 caactgaaca ggaagctcaa tatagtttac ttttgttatg gccttgatac ctaccggaat
    15901 attgaaatcc aacacattga aagctgtacc ggcttttgct tcgaaaccac gttgtgcata
    15961 atttaactcc ggctgaaacg agaattgttc gttgattctg tactgcaagt ttacaccggc
    16021 gagtggcgta ttgattattt tagcgtctgg aagcagtgtt tcagaaattc cagaaagact
    16081 cgcgtctgca aaattaatac ccgtctttac acctacggag atctgggcat ttacaaataa
    16141 agaagcaaac atagttgctg ccaaaatgat aattttcgtc ttcatgatct attcttttat
    16201 ttatcattat gacgcgtagt atcaggccat tttggttaat acaatattaa ggtttgttag
    16261 tagtacctta agaattagta aggttgatca ctttttggtt aaaaaaatga taaaaccacc
    16321 atcataccta tttctgaaag gtattcaatt ggtgtgaatg gatctaacct actaatttaa
    16381 taatatagac aacaaagaaa accaaacctg ctattgccag cagagaagaa ataaagccaa
    16441 gtaaataaat tcctccaaga agccagctga caaggccaac acctatggct cctgcaaagc
    16501 aaaggattgc ccacgatagc cagccatcta cagggtcgct aagatcaaac gtaaaaaaat
    16561 gtctgatttt tttcacgatg gttttaatag aatttttttt gaccacgttt gtgtggttgt
    16621 acgatgtgcc attttttgca gtaagggata aagtatctgt ctttactact actacagcat
    16681 tggcgtacga atgagctaaa aggacaatgg atatgataaa taaaatctgc ttcataatgc
    16741 gagttattgt gtcgtattgt tttaaataat aataatgatg tcaactaagt tgtgattttc
    16801 tgcatactaa tattgtgtgt aaaaacttgc gtaactaaat ttaaaccttc agagtacaca
    16861 actaattata gttttttaaa ttcatttttt tgagaaaata aaaatagata aaaccaaccg
    16921 atgataaaac atacaccacc tgccggagtt actggcccta atattcccag aggcaaaaaa
    16981 gcaagaaaat ccttaagtga tattagatac aatgatccgg aaaacagaaa gatgccgatg
    17041 gtaaatagct ttgtggctcg gttaagattt ac
//
LOCUS       DAOQQY010000026        16963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1057_length_16963_cov_5.349716, whole genome shotgun sequence.
ACCESSION   DAOQQY010000026 DAOQQY010000000
VERSION     DAOQQY010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16963
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1057_length_16963_cov_5.349716"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PLE23_02725"
     CDS             complement(<1..207)
                     /locus_tag="PLE23_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82210.1"
                     /translation="MSRIKLILFNFGLLTLAALFMSYNANPPDGKTGAPGEGTCGDCH
                     SGGTSTADVEILGLPASINANEAYN"
     gene            complement(527..1339)
                     /locus_tag="PLE23_02730"
     CDS             complement(527..1339)
                     /locus_tag="PLE23_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82211.1"
                     /translation="MSRIRLILLNFGLLCLAATFMSYNSNPPEGKTGAPGESTCSDCH
                     SGGSSTGNVEILGLPSVINAKEFYNINVKVTKTGGNATKAGFQLVALNAANQNAGDLS
                     TLASDHGTNSENGREYVEHRNGKAFVNNVAEWTFKWQAPDAPDSSNITMYAVGNICNG
                     NNNTSGDKVVKVNATGKLKVKSTSTNDLISHGWNLYPNPSKDFLYLQRPTDNTQPVSW
                     QVFDINGSLLKSGESNSILGDIPIEVSTFVIGQYFLRLNSGTDSYYSKFVKL"
     gene            complement(1405..2364)
                     /gene="ftsY"
                     /locus_tag="PLE23_02735"
     CDS             complement(1405..2364)
                     /gene="ftsY"
                     /locus_tag="PLE23_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HMX82212.1"
                     /translation="MSFFKKFFSKDKEKDLDKGLEKTKEGFFDKIAKVVIGKSTVDES
                     VLDELEEVLISSDVGLETTVKIIDRLNERVARDKYLSTNELDTIMRDEIVKLLTENNT
                     ADFEDFELPRDKKPYVLLVVGVNGVGKTTTIGKLAYQYSKKGYKLVIGAGDTFRAAAV
                     DQLKIWSERVGCHFYAKGMNTDPAAVAYETVQYAINNQLDMAIIDTAGRLHTKTNLMQ
                     ELTKIKRSIDKCMEGAPHDVLLVLDATTGQNAIEQCKQFSAATDVSELALTKLDGTAK
                     GGVAIGISDQFKIPIRYIGVGEGIENLQIFNKKAFVESLFRKS"
     gene            complement(2490..2666)
                     /locus_tag="PLE23_02740"
     CDS             complement(2490..2666)
                     /locus_tag="PLE23_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 family protein"
                     /protein_id="HMX82213.1"
                     /translation="MAKISKNARVAQRAASAGSGKDHVRVIKSIKDPVTGKYTYKESI
                     VHKDKVKEFFENTK"
     gene            complement(2693..2881)
                     /gene="rpmG"
                     /locus_tag="PLE23_02745"
     CDS             complement(2693..2881)
                     /gene="rpmG"
                     /locus_tag="PLE23_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HMX82214.1"
                     /translation="MAKKSKGNRIQIILECTEHKTSGMPGTSRYVTQKNRKNTPERLE
                     IKKYNPILKKMTVHKEIK"
     gene            complement(2910..3158)
                     /gene="rpmB"
                     /locus_tag="PLE23_02750"
     CDS             complement(2910..3158)
                     /gene="rpmB"
                     /locus_tag="PLE23_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HMX82215.1"
                     /translation="MSRVCQLTGKKSIVGNHVSHSNIKTKRRFYANIQTKRIYVPELD
                     KWVTLNVSTHALRTISKLGLLEYVKKLEKKGIDTGIRF"
     gene            complement(3205..3278)
                     /locus_tag="PLE23_02755"
     tRNA            complement(3205..3278)
                     /locus_tag="PLE23_02755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3242..3244),aa:Arg,seq:acg)
     gene            complement(3368..4951)
                     /locus_tag="PLE23_02760"
     CDS             complement(3368..4951)
                     /locus_tag="PLE23_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMX82216.1"
                     /translation="MKHPLIFFLLYSLFNLTSTFGQSGFIVQYEEGFRSLENAVRQDG
                     TFYVADHIPFTDIFLLKSVGDRSSNKSDLLKLNIPFRSVSVCRQVKPRTITPDDPYYK
                     DQWQYDILHAGEAWEFGTGGTTCAGDTIVMAVLDEGYELSHDDLKSNIWINHGEIPND
                     GIDNDNNGYIDDVQGLNTGTNTDKHDHSSHGLKVAGIMGARANNQKGVAGVNWTSKIM
                     YVSYIADDFYILKGLHYILTQRRKYNESNGKEGAFVVSVNNSFGIPNLFPEDGLELWC
                     NMYDSLGMAGIISVGSTTNSKTDVDVAGDIPSTCPSQYLIIVTNTDKNDKKVNNAGYG
                     SKSVDIGAPGEGVFTVGLNNAYTTFTGTSASAPHVTGAVGLLYNINCCELANMAISEP
                     AKAALRVKDDILKGATPLTSLNNITTNGGRLDLYGAATALTSTCGNALGTDKINDIQL
                     LNLGDRIKVQFTGSAYKTFQYSVYNMLGMELANGEFDYSAFGPDSFDVDMTGLPPAVY
                     FISIFGGKSPISMNFIKLK"
     gene            complement(4948..5760)
                     /locus_tag="PLE23_02765"
     CDS             complement(4948..5760)
                     /locus_tag="PLE23_02765"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HMX82217.1"
                     /translation="MIQKYSDSFFIDQMGGQISLGNPPKRIISLVPSQTELLYELGAA
                     DLLVGRTKFCVHPKPDVLKLPVVGGTKTPDLQRIIKLNPDLIIGNKEENEQISIEEIK
                     RHFPVWMSDIENFNQSIDMIMMLGSLLVCEENVSTLVEQILVAKDELQTFVGAKFGNH
                     KPEVIYMIWKNPYMAAGTNTFINEMLGFCGLKNLAGGRYPEFSNEQLYALNPELIFLS
                     SEPYPFKNKHVSEIKEICPDARIVLVDGEMFSWYGSRMKAAFPYFKQLLQTL"
     gene            complement(5762..6379)
                     /locus_tag="PLE23_02770"
     CDS             complement(5762..6379)
                     /locus_tag="PLE23_02770"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HMX82218.1"
                     /translation="MNIFLRFFFFPVLIVSFVQVSSIAQSTYFDEIMAYRNTYINNFL
                     TEKDSPLDSSGVKLLDFFEPDSTYRVIAKVKMRKRQKVFDMATSDGTSKTFREYARLH
                     FVVNNTKVILPVYQYLGRNMSVLHPEHLFLPFTDYTNGEETYGGGRYIDLTIKDIHNG
                     MVTIDFNKSYNPYCTYKEGYRCPVPPKANAIKTEIKAGEKAYKHE"
     gene            6493..6897
                     /locus_tag="PLE23_02775"
     CDS             6493..6897
                     /locus_tag="PLE23_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMX82219.1"
                     /translation="MLNEPVSKIMKTNLYTATAEDSLEKIHSLFREHRIHHVPVVDGV
                     KLVGLITTQDLLKSNLCKENYGDAKVGDYMTKKVATVETDAKIGTAAEIFLENRFHGL
                     PVVNEDRELLGFLTTFDVLNYVFRKEYPNNPW"
     gene            complement(6996..8567)
                     /gene="lepB"
                     /locus_tag="PLE23_02780"
     CDS             complement(6996..8567)
                     /gene="lepB"
                     /locus_tag="PLE23_02780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMX82220.1"
                     /translation="MVVLIFLIVTYLLFCFTLKPLFEKAGIEGNKAWIPWVNFKEAFK
                     MVGDKPNRVWWLLFPIYNLFVYIGLCIDIVRSFGRYSFADSAQAFIYAPFIFFNIGKD
                     PKSKYVGPTLKLEKEYHDRITEAQRKNDLVTLKRLDAANPYKKGMLREWTEAAVFAIF
                     AAAFIRMFLIEAYQIPTSSMEGSLLVGDYLFVSKANYGIRTPETVAMVPLVHNRMPFF
                     DGESYFKWPKLPYFRLPALEKIKRNDPVVFNYPEGDSVYFDPQRTWTIYDVRRDPAKA
                     QFTVNKPLTVRPVDKEDHYIKRCVAIAGDTFEIRNREVFINGKATVAPRFVQFTYIVS
                     NPDGAVNMSSFDDWGISPEDVLDVQGNNMALILNNEQIEKLKSQNPNIRIEPYKIPDR
                     PLFPYDTAHFKGFSVDNYGPLWIPKKGATLKISQDNIAAYRRVISVYEGNKLEEKAGK
                     IFINGKEASEYTFKYDYYWMMGDNRHNSEDSRYWGFVPETHIVGKPLFIWFSTKYADM
                     KKGIRWNRIFTSANK"
     gene            complement(8713..9132)
                     /locus_tag="PLE23_02785"
     CDS             complement(8713..9132)
                     /locus_tag="PLE23_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMX82221.1"
                     /translation="MQVAFFEKSTVNTVRCGFFYSIFAVIFFSFSTADKASAQEVIWQ
                     DSLSYDFGEISQNHPVTHTFHFTNMNTDTIDIETVRSDCGCTETDWTKEEIPPGSAGK
                     VIVIFDSKHVGPFKKKVRVYFRQFKKAHILHILGQVL"
     gene            complement(9132..10094)
                     /locus_tag="PLE23_02790"
     CDS             complement(9132..10094)
                     /locus_tag="PLE23_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82222.1"
                     /translation="MSAQNDNRNKILGIAIAAIIALLGVNAYLLYNKSKLTTANTQLS
                     TQLDETSKLKLELEQQYNESLSKLEEMRGDNEEMNAMIDKQKSDLQAQKNQISNLLKN
                     KGDLSKAKEQIAQLISQRDQFMAQLEELKMKNEQLTADNTKLTTDLQSEKANTESLTA
                     AKAALVSEKDNLASQNEKLSSKVTRASVIPVSKVEITGLKVDSKNKEHKKRYAKNIDR
                     LRVCFDAAANDITEGGNETFFIRMINPTGETLAREDMGSGTLTTTDTKEKIRYTSSKD
                     IEYSKTATQVCLNWDQAEALAKGVYQVEIYNKGYKCGEGSFKLK"
     gene            10197..10934
                     /locus_tag="PLE23_02795"
     CDS             10197..10934
                     /locus_tag="PLE23_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HMX82223.1"
                     /translation="MLQNIDLSKILFLDVETVSQKSSFDELDETFQSLWTRKSRQFLK
                     DNTEDPDAVKETYTDKAGIFSEFAKVVCISVGFLRMEGKKMVFRLKSFYGDDEAEVLR
                     DFSNLLNKNYNNPLSSFLCGHNIKEFDIPFLCRRMIINGLEIPEIMQVSGKKPWEIKH
                     FIDTLELWKFGDIKNFTSLNLLAAVLGVPSPKDDIDGSQVGRVYWQEKDTERIAQYCQ
                     KDVQTCMQILLRLQGKDLLPEDQIEKP"
     gene            10931..11605
                     /gene="rpe"
                     /locus_tag="PLE23_02800"
     CDS             10931..11605
                     /gene="rpe"
                     /locus_tag="PLE23_02800"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMX82224.1"
                     /translation="MTKHLIAPSLLSADFRNLARDMEMINNSEADWFHVDVMDGNFVP
                     NISFGMFIVEFMKSVAKKPLDCHLMMVQPERYIAEFRKAGADVITVHYEACPHLHRTV
                     QQIKQTGARAGVALNPHTPVEVLQDIIIDLDIVLIMSVNPGFGGQSFIEQTYNKVKRV
                     KSMILATGSTALIEVDGGVGLGNTKRLVECGADVLVAGNAVFKAENPAQTIHQLKNLT
                     DKTYLV"
     gene            11639..14173
                     /locus_tag="PLE23_02805"
     CDS             11639..14173
                     /locus_tag="PLE23_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX82225.1"
                     /translation="MQIFLKRFLFIVMVLNCVAAFAQYKTVVTGRVADLETNQGIEFV
                     NIRIDGTTRGTESDEDGNFEIVVNTNEKANLIFTRIGYKQYTHSIPSFSQGQLKLDIK
                     MVPADSKMEVVITEEKIISGGMVRQNVEELKLLPSASGNLESALPSIALGTSSGTGGE
                     LSAQYNVRGGNYDENLVYINDFEIFRPQLIRTSQQEGLTFPNIDLIRDLSFSSGGFQA
                     KYGDKLSSVLDINYKRPEKIKASVSGSFLGGSAHLEGSRPIHRDTYRKFRYLVGARYK
                     TSKYLLNSLEVEGEYVPTYTDIQTFLTYDITRSLQLGIIGNYNKSNYYFKPESSSSGA
                     GLVNFALRLSTAYEGQEADDFAQMMGGLSLTFVPDTKKNPYFIKLLAAAHRGYEDETY
                     DILGYYSLGQVETNLGSDKAGQVVAVLGEGTQHNYARNHLISTVKNLELRGGHDFQGK
                     NEDGKSANNFLEWSLKAQSEEISDKLNEWERLDSAGYSLNYSLENLYLKSVLKTKNQI
                     NSIKYSAFIQNTYTIKTDDKAEWKLNLGVRAMYRDLNSELLVGPRAQVQFRPLSWHND
                     WAFRLSGGYYYQPAFYREMRRPNGTVNTGLLSQKSAQIVGGISRQINWTRISDKPFTL
                     IAEMYYKKLWDVVSYEVDNVRIRYSGENNATGYVTGFDLRLNGEFVPNAESWINLSFL
                     RARENLDGVQHKIREVGQAEGKDVADVPRPTDQLFNVNIFFQDYLPRNENFKVHINLA
                     VGSGLPFGIKDNNQVYRNTYRYSAYHRADIGFSLLLWDKKWKDRKPHHFLRFSENTWV
                     SLEIFNLLNVQNTASNNWIKTVFNTQYAIPNRLTSRRINLRLRMDF"
     gene            14196..14645
                     /locus_tag="PLE23_02810"
     CDS             14196..14645
                     /locus_tag="PLE23_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMX82226.1"
                     /translation="MKNLFQKEILEEVLTRIEHLTPDTPARWGVMNVAQMLAHCAESV
                     DMATGTNKPTRMLLGRIFGKLFRPVYSNAIPFMKNLPTAKSLRIRDERDFNNEKEKLI
                     TKLKQFSKGGEAVCTSFPHPLLGRLTPNEWSVGTYKHLDHHLRQFGV"
     gene            14754..15182
                     /locus_tag="PLE23_02815"
     CDS             14754..15182
                     /locus_tag="PLE23_02815"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HMX82227.1"
                     /translation="MSVADKDISTNQKTMVDIIRFFKFGLVGSVGILIDFSLTWLFRD
                     RLKWNQYIANIIGFCVAASSNFVLNKIFTFQDQDHTIITQYLTFFMISCIGLIMNSLF
                     LLMLQKKTNLNFYFSKILVTGLLFLWNYAANSIYTFNTTT"
     gene            15179..16720
                     /locus_tag="PLE23_02820"
     CDS             15179..16720
                     /locus_tag="PLE23_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMX82228.1"
                     /translation="MSLSQKTILLIIIATGLRIIFAGSIELGNDEVYYITYAQKLQWN
                     YFDHPPLVGLLIKITSLNLWLTNEIFIRLGPILLAAVDTWLIFRIGTHIKNEKAGYLA
                     ALFFTASPYTSIISGLFIMPDAPMLFFWLLSIWLLVRVFDPNISKRQSNISLLLFGLT
                     AGLAIMGKVHAIFLWVGLGIYLLLYNRRMLLNPVLYLAGVISMAIISPIYFWNAQNDF
                     ITYKFQGSRVTVNSGFRADSLLTELFGGILYNNPVNYVLILIALIAIFRKKRPSVFPY
                     RNLLLCLSLPLIGILIFVSCFRSTLPHWNGPGFVVLSIVASAWLAETHFTKYNKWLTT
                     ANIVLLLIAIVAVLGINFYPGTPGNKDYAKLGEDDLTLEMYGWKQFNQSFRQMYEDDT
                     RSGKMKKNSAIATHKWFPGGHLNFYVALPDSITLIGLGTLNDIHHFYWLNEVRPKLKK
                     GDDAYIIVPSNLNYSVRVDDTYGQMFNAIEKPSIIDQLRNGKPARYFSVYRAHGFRPP
                     EAVGY"
     gene            complement(16714..>16963)
                     /locus_tag="PLE23_02825"
     CDS             complement(16714..>16963)
                     /locus_tag="PLE23_02825"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HMX82229.1"
                     /translation="IQAPVIMMSQNRQEEKDRIRAENDYLINLKAEMEILRLHQKVDL
                     LLEEQIKTLFETQARQLNLLNEIHKKMEKHPSPGSAHQ"
BASE COUNT         4894 a         3390 c         3382 g         5297 t
ORIGIN      
        1 attgtatgcc tcattagcat ttatggaagc aggtaaacca aggatttcga catcagctgt
       61 agatgtacca ccgctatggc agtcgccgca tgtaccttcg ccgggagctc ctgtcttgcc
      121 atcaggcgga ttggcattat aagacatgaa gagtgcggcc aatgtaagta agccgaaatt
      181 gaacaagatg agtttgattc gtgacatgtt taaatttgtt ggttagaaca aaaataacac
      241 gagccataaa aagttgttca gttttttcaa gattttctga aaattgaatt tacattttct
      301 tgtaaaagta tttttgagtc acaatttgtt ctctgttgat gcaattcaat tcttctcctt
      361 ttctagaatg aactatatga aacacaatgt gacaggattc tctgtgccaa tttcaaaatt
      421 ttagcatcct aattttttgt aaagtcagga gcaaagattc agactcattt aaaaaaataa
      481 aaggctttat agtattgcag ccgaagctaa ccggtaaaaa tacaaattaa agttttacga
      541 acttcgagta atatgaatct gtgccagaat tgagtctcaa gaaatattga cctatcacaa
      601 aagtgcttac ttctatggga atatcgccta aaatgctatt gctttcgcca gatttcagaa
      661 ggctgccatt tatatcaaaa acctgccatg agactggctg agtattatct gtaggcctct
      721 gaaggtaaag gaagtctttg gacgggttag gataaagatt ccatccgtgt gagattaaat
      781 cattggttga tgtgcttttt actttcaatt ttcctgtggc attcactttg acaactttat
      841 caccggaagt attattattg ccattacaaa tgttacctac ggcatacata gttatgttag
      901 aactgtcagg tgcatcgggt gcctgccatt tgaaggtcca ttctgcgaca ttgttgacaa
      961 atgctttacc atttctatgc tcgacatatt ctctgccatt ctccgaattg gtaccgtggt
     1021 cgctagccaa tgtgctcagg tctccggcat tttggtttgc tgcattaagg gcaacgagct
     1081 gaaaaccggc ttttgtagca ttgcctcctg tttttgtgac ttttacatta atgttgtaaa
     1141 attctttggc attaataaca gatggcagcc caagtatttc aacattacca gttgaagaac
     1201 cgccactgtg acaatcgctg caggtagatt caccgggtgc accagttttg ccttcaggtg
     1261 ggttggagtt ataggacata aaagtggctg ccaagcagag taagccaaag ttaagcaaga
     1321 tgagtctaat acgtgacata gtgtattaca tttgttggtt aatttaaacg taacagtatt
     1381 tgaaaaaaca agttcagttt tttttcaaga ttttctgaac agagattcta caaatgcttt
     1441 tttattaaaa atctgaaggt tttcgattcc ttctccaacg cctatgtaac gaatgggtat
     1501 cttaaactgg tctgagattc cgatggccac accacctttg gctgttccat cgagtttggt
     1561 aagtgccagt tcagacacat ctgttgctgc agagaattgt ttgcactgtt cgatggcatt
     1621 ctggcctgta gtggcatcca gcacaagcaa tacgtcatga ggggcgcctt ccatacattt
     1681 atcgatggag cgttttattt tagtaagctc ctgcatgagg ttggttttgg tatgtagtcg
     1741 gccggctgta tcgattattg ccatatcgag ttggttattg atggcatatt gtacagtttc
     1801 gtaagcgaca gcagcagggt cggtgttcat tcctttggcg taaaagtggc aacctactct
     1861 ttcagaccat attttcaact gatcgacagc tgctgccctg aaggtatcgc ctgctccgat
     1921 aaccagtttg taaccttttt tagaatactg gtaggcgagt ttgccgatag tggtcgtttt
     1981 gccgacacca ttgacaccta caacgagcag tacataaggt tttttatcgc gaggcaattc
     2041 aaaatcttca aagtcggcag tattattctc agtcaggagt tttacgattt catcacgcat
     2101 gatggtatcg agttcgtttg tgctcagata tttatcacga gctactcttt cattgagccg
     2161 gtcgatgatt tttacagttg tttcaagtcc cacatccgag cttatgagta cctcttcgag
     2221 ttcatccagt acactttcgt ccacagttga tttgcctatt acaactttgg ctattttatc
     2281 aaaaaaacct tctttagttt tttcgagtcc tttgtcgagg tccttttctt tatcttttga
     2341 gaaaaacttt ttaaaaaagc tcatgttcgg tggtttgtta agctgcaaat atatttaaaa
     2401 tctcccaaaa tgcacggaag atgtgagagg tagaaacaca aaaaggcttc cccggcagga
     2461 aaagccttta ttgcattaga atacaaatat tacttagtgt tttcgaaaaa ctctttcact
     2521 ttgtctttgt gcacgataga ctctttatag gtatatttac ctgttaccgg gtccttgatg
     2581 ctttttatta ccctcacgtg atcttttcca gagcctgcac ttgcagcacg ctgagcaacc
     2641 cttgcgtttt tggatatttt agccatgaca gaatattttt aaggtgtaaa aattatttta
     2701 tttctttgtg aacagtcatt tttttcagga taggattgta ttttttaatt tcaagccttt
     2761 ctggagtgtt ctttctgttc ttctgggtca cgtaacgtga agtaccaggc ataccactgg
     2821 ttttgtgctc agtgcactcg aggatgatct ggattctgtt accttttgat ttcttagcca
     2881 ttatctaagt atttttaaat ttttctctat taaaaacgaa tacctgtgtc aatccctttt
     2941 ttctcaagct tctttacata ctctaacaat cccagctttg aaattgttct taaagcatga
     3001 gtagaaacgt tcaatgttac ccatttatcc aattcgggta catatatacg cttggtctgg
     3061 atattagcat aaaatcttct cttggtcttg atgttcgagt gcgatacatg attaccaaca
     3121 attgatttct ttcctgttaa ttgacaaact cttgacatgt ctgtgttctt ttatgtatgg
     3181 tttataaatt ccaaataaat taagtgactt cggcaggatt cgaacctgca accttctgat
     3241 ccgtagtcag atgctctatc caattgagct acgaagccaa gttcctcctt ttcgggatgc
     3301 aaaattaata gtttttatta taagttgaaa tttttttcgc ttttaatttc gaaaaattga
     3361 ctttttttta ttttagcttg atgaaattca tacttatagg gctttttccg ccgaatatgc
     3421 taataaaata tacagctggt ggcaatccag tcatatctac atcaaagcta tccggtccga
     3481 atgccgagta atcaaactct ccattggcca attccattcc aagcatgtta tatactgaat
     3541 attgaaatgt cttgtaagca gaaccggtaa attgaacctt aattctatca ccaagattaa
     3601 gaagttgaat atcattaatc ttatctgtcc ctaaagcatt gccgcacgta cttgttaagg
     3661 ctgttgcagc accatacaag tcgagcctac ctccattggt agtaatgttg tttagtgatg
     3721 tgaggggcgt tgctcctttc aaaatatcat cctttaccct gagggcagcc ttggctggtt
     3781 cggatattgc catattggct agttcacaac aattaatgtt gtagagcaat cctactgcac
     3841 ccgtgacatg tggtgcagac gccgatgtgc cagtaaatgt agtatatgca ttattgagcc
     3901 ctaccgtaaa caccccttca cccggcgcac ctatatctac cgattttgaa ccataacctg
     3961 cattgtttac cttcttgtca tttttgtctg tgttggtaac aataataagg tattggcttg
     4021 ggcaagtact cggaatatcg ccagctacat ccacatctgt tttgctgttg gtagtagaac
     4081 ccacgcttat tatacccgcc atgccaagtg aatcgtacat attacaccac agttcgagcc
     4141 cgtcttcagg aaaaaggttt ggaataccga atgaattatt gaccgaaacc acaaaagcac
     4201 cttccttacc attggattca ttgtattttc tcctttgtgt taaaatgtaa tgcaaaccct
     4261 tgagaatata aaaatcatcg gctatgtacg aaacatacat aatcttgctt gtccagttga
     4321 caccggctac acctttttgg ttattagctc gagctcccat gatgccggcc accttcaatc
     4381 catgtgaaga gtgatcatgc ttgtcagtat tcgtacctgt attgaggcct tgtacatcgt
     4441 ctatgtatcc attgttatcg ttgtctattc cgtcattagg tatttcgccg tggtttatcc
     4501 atatattgga ctttaaatca tcatggctga gttcgtagcc ttcgtcgagt actgccataa
     4561 cgatggtatc gccggcacat gtagtacctc ctgtaccaaa ttcccaagcc tcaccggcat
     4621 gtagtatatc gtattgccac tggtctttat agtatgggtc atcgggagta atggttctcg
     4681 gctttacttg tctgcaaaca gaaaccgacc ggaatggtat attaagcttg agtaaatctg
     4741 atttgttgct acttctgtca cccactgatt ttaacagaaa aatatctgta aaaggaatat
     4801 ggtctgccac ataaaaggta ccatcctgac ggacagcatt ttctaaagat ctaaagcctt
     4861 cttcatattg cacaataaaa ccagattggc caaatgtgct tgtaagatta aataaactgt
     4921 aaagcagaaa aaatataagt ggatgcttca taatgtttgt aataactgtt taaaataagg
     4981 aaatgctgcc ttcatcctgc tgccatacca tgaaaacatt tctccatcga ctagcacgat
     5041 tctagcatct gggcatattt cttttatttc tgaaacgtgt ttattcttaa aaggatatgg
     5101 ttctgaagat agaaagatca attcaggatt aagtgcgtat agttgttcat ttgaaaattc
     5161 cggataacgt ccacctgcaa gatttttcaa accacaaaaa ccaagcattt catttataaa
     5221 tgtattggta ccggcagcca tataaggatt tttccatatc atatagataa cttccggctt
     5281 atgattgccg aattttgcac cgacaaatgt ttgaagttcg tccttggcta ccaatatttg
     5341 ttctacaagt gtagatacat tttcttcaca tacaagcaag cttcctagca tcattatcat
     5401 gtcaatactc tggttaaaat tttcgatgtc actcatccaa accggaaaat gtctttttat
     5461 ttcttcgatc gaaatttgtt cgttttcctc tttattacca attataagat ccggattaag
     5521 cttaatgatg cgttgcagat cgggtgtttt ggtgccacct actacgggta gtttcagcac
     5581 atccggcttt ggatggacgc aaaacttagt acgcccgacc agcagatctg cagcacctaa
     5641 ctcatataag agctccgtct gagatggaac cagagatatg attcttttag gtggattacc
     5701 aagtgagatt tgcccgccca tttggtcaat aaaaaagcta tcggagtatt tttgtatcac
     5761 ttcattcgtg tttgtatgct ttttcgcctg cttttatttc ggttttgatg gcattggctt
     5821 ttggtggtac cggacaccga taaccttctt tgtaggtgca gtaaggattg taagatttgt
     5881 tgaaatctat tgtcaccata ccattgtgta tatctttaat agtcagatcg atatatctgc
     5941 cacctccata agtttcttcg ccattggtat aatcggtaaa tggaagaaaa aggtgttcgg
     6001 ggtgcaatac gctcatattt ctgccaaggt attgataaac aggtaatata acttttgtat
     6061 tattgacaac aaagtgcaac cgggcatatt ccctgaatgt ttttgaagta ccgtcactgg
     6121 tagccatatc gaagactttc tggcgttttc gcatcttaac tttagcaatg acacgataag
     6181 tgctatcggg ctcaaaaaag tctaataatt ttacacctga agagtcgaga ggcgaatctt
     6241 tctcggtgag aaaattgttg atgtaagtat tacggtaggc cattatttca tcaaaataag
     6301 tcgattgggc aatagatgac acttgtacga aacttacaat taatacagga aagaaaaaaa
     6361 acctcagaaa tatattcata agctgatatt tatgattcaa aattgcatat ttttgtcaaa
     6421 tattatgaaa ttcattatgt ttttaacaaa aacttttgta tcttagacct aaattttaaa
     6481 aactgacaca ctatgttgaa cgaacctgtt tcaaagatca tgaagacaaa tctctacaca
     6541 gcaacagctg aagattctct tgagaagatt cattcgctgt tcagagagca cagaatacac
     6601 cacgtaccgg tagtggatgg tgtcaaactt gtaggcttga ttacaacaca agatttgctg
     6661 aagagcaacc tttgcaagga aaattatggc gatgcaaaag ttggtgatta catgactaaa
     6721 aaagtagcaa ccgtagaaac ggatgctaaa attggtacgg cggcagaaat tttcctcgaa
     6781 aacaggtttc atgggttacc agtagtaaac gaagaccgtg agttgcttgg ttttctaact
     6841 acttttgatg tccttaacta tgtattcaga aaagaatacc cgaataaccc atggtaagac
     6901 cggtgtagta ttcctagatt caaaaaagcc acgattctga catcgtggct ttttcatttt
     6961 atcaatttct atttttactt cattacagct cagctttatt tattggctga tgtaaaaatc
     7021 cgattccatc ttataccttt cttcatatcg gcatacttag tcgaaaacca gatgaacaat
     7081 ggcttaccaa cgatatgcgt ctccggtaca aaaccccaat agcggctatc ttcactgttg
     7141 tgtcggttat ctcccatcat ccaataataa tcatacttga atgtatattc ggaggcttcc
     7201 ttgccattga taaatatttt accggccttt tcttcaagtt tatttccttc atataccgaa
     7261 atgacacggc ggtaagcggc tatattgtct tggcttatct ttagtgtagc gccttttttc
     7321 ggtatccaga gcggtccgta attgtcaacc gaaaatccct tgaaatgtgc tgtatcatac
     7381 gggaaaagcg gtcgatcggg tatcttgtat ggttctatcc tgatattcgg attctgtgac
     7441 tttagttttt ctatttgttc gttattgagg atgagtgcca tattattgcc ttgtacatcg
     7501 agcacatctt caggcgaaat gccccaatca tcaaatgaag acatattgac agccccatca
     7561 ggattggata cgatgtaagt aaactgcaca aagcgtggag ctaccgtagc ttttccattt
     7621 ataaatactt cgcggttacg gatttcgaaa gtatcgcctg ctatggcaac acatcgttta
     7681 atgtaatgat cttccttgtc caccggtctg actgtaagtg gtttgtttac ggtaaactgt
     7741 gccttggccg gatcacgtct tacatcgtat attgtccagg ttcgttgtgg gtcgaagtaa
     7801 acactgtctc cttccggata attaaatacc accggatcat ttctttttat tttttcaagt
     7861 gccggcaagc ggaaataagg taatttaggc catttgaaat aagattctcc atcgaagaat
     7921 ggcattctgt tgtgtaccag tggcaccata gctaccgtct cgggtgtacg aataccataa
     7981 ttggctttgc tcacaaacag ataatcacct accagcaggc ttccttccat agaagaagtc
     8041 ggtatctggt aagcttcaat caaaaacatc ctgatgaatg cagcagcgaa aatggcaaaa
     8101 actgcggctt ctgtccattc acgaagcatc ccttttttgt aaggattagc agcatccaat
     8161 cgtttgagtg tgaccagatc atttttgcgc tgcgcttctg ttattctatc gtggtattct
     8221 ttctcgagct tcaatgttgg tcctacatat ttactttttg ggtccttgcc aatattaaaa
     8281 aatataaatg gggcataaat aaaggcttgt gctgaatcgg cgaaactgta tcttccgaac
     8341 gacctgacta tgtcaataca aagcccaata taaacaaaaa gattataaat gggaaaaagt
     8401 agccaccata cacggttcgg tttgtcacct accattttaa aggcctcctt aaaattgacc
     8461 cagggaatcc atgctttatt cccttctatt ccggcttttt cgaaaagcgg tttgagtgta
     8521 aaacagaaaa gcaggtaagt tactatcaga aaaataagta caaccattgt atttttttaa
     8581 aaatttgtgc aaagataaat gaaaattcca tcccgaaaac gtcttttcgt tctacctctg
     8641 tatatcatgg ttcaattcat tcgacgaaat tattagaatg aactacaaaa caagacctta
     8701 caccggacaa tttcataaaa cttgtcctaa gatgtgcagt atgtgtgctt ttttgaattg
     8761 tctgaaatag accctgactt tcttcttgaa cggccctaca tgtttactgt caaatattac
     8821 gatgactttt ccggctgacc cgggtggtat ctcttctttc gtccagtcgg tctctgtaca
     8881 gccacagtct gagcgcacgg tttctatgtc gatggtgtct gtgttcatat tcgtaaaatg
     8941 gaaagtgtgg gtcaccgggt ggttttggct tatttcacca aaatcataac taagactgtc
     9001 ttgccaaatc acttcctgag cacttgcctt gtccgctgtg gagaaagaaa agaaaataac
     9061 cgcaaagata gaatagaaaa atccacagcg aaccgtgttt actgtggatt tttcaaagaa
     9121 agcgacttgc attatttcaa cttaaatgag ccttcaccac atttatagcc tttgttgtag
     9181 atttctactt gataaacccc ttttgccagt gcttcggcct ggtcccagtt gaggcatact
     9241 tgtgttgcag tcttgctata ttctatatct tttgaagagg tatagcggat tttttcttta
     9301 gtatctgtag ttgtaagtgt gccactaccc atatcttcac gtgcaagtgt ttctccggtc
     9361 ggattgatca ttctgatgaa gaaagtttca ttgccacctt cggtgatatc attggctgct
     9421 gcatcaaaac aaacacgtag tctgtcgatg tttttggcat aacgtttttt gtgttcttta
     9481 tttttgctgt caacctttaa gcctgtgatt tcgactttgc ttacaggtat tacagaagct
     9541 cttgttactt ttgaagacaa tttttcgttt tgtgatgcaa gattatcttt ttcactcacc
     9601 aaggctgctt tagctgcagt caagctttct gtattggcct tttctgactg gagatcggtc
     9661 gttagttttg tattatcggc agtcaactgt tcatttttca tcttcaactc ttctagttgc
     9721 gccatgaact gatctctttg gctaataagc tgagcgattt gttctttcgc cttacttagg
     9781 tcacctttgt ttttaagcaa atttgaaatc tggttttttt gtgcctgaag gtcagatttt
     9841 tgtttgtcaa tcatggcatt catttcttca ttatcgccac gcatctcttc aagtttgctg
     9901 agcgattcgt tgtattgttg ttctagttcg agttttagtt tgcttgtttc atcaagttgg
     9961 gtactcaatt gagtatttgc tgttgtcaat tttgacttat tgtatagcaa ataagcattt
    10021 acacccaaca aagcaatgat ggcagcgatg gcaataccta agattttatt gcggttgtca
    10081 ttttgggcac tcataatgtt caaattttgg ttttgattaa aagttaatta atttcgcatt
    10141 gtataacgca aaattaatat attaaagtat atttgaatgt taacaaactg taaaaaatgc
    10201 tacaaaacat cgatctaagt aaaattttgt ttctcgacgt cgaaacggta agtcaaaaat
    10261 ccagttttga cgaactcgat gaaacatttc aaagcctgtg gacacgtaaa agcagacaat
    10321 ttctgaaaga caataccgaa gacccggatg ccgtcaaaga aacatatact gataaagccg
    10381 gcattttttc tgaatttgca aaggtagtct gtatctcagt tggctttttg agaatggaag
    10441 gaaaaaaaat ggtattccgt cttaaatcct tttatggcga tgatgaagct gaggtattac
    10501 gtgatttttc taatttgctg aacaaaaatt acaacaatcc gctcagttcg tttctgtgcg
    10561 gacataatat taaggagttt gatattcctt ttttatgtcg gcgaatgata atcaatggct
    10621 tggaaattcc tgaaataatg caggtatcgg gcaaaaagcc gtgggaaata aaacatttta
    10681 tcgacacact tgagttgtgg aaattcggtg acatcaaaaa ttttacaagc cttaacttac
    10741 tggcagctgt gctcggtgtt ccatcaccaa aagatgacat tgatggtagc caggtggggc
    10801 gtgtatattg gcaggaaaaa gacaccgaaa gaattgcaca gtattgtcag aaggacgtac
    10861 agacttgcat gcagatactt ctacgactgc aggggaaaga tttattacca gaggatcaaa
    10921 tagaaaaacc atgacaaaac atttaatagc accatcgtta ttatcagccg atttcagaaa
    10981 ccttgccaga gacatggaaa tgattaacaa tagtgaagcc gactggtttc atgttgatgt
    11041 gatggatgga aacttcgttc caaacataag tttcggaatg tttatagtcg aattcatgaa
    11101 atcagtagca aaaaaaccac ttgactgcca cctgatgatg gtacaaccgg agcgttatat
    11161 tgcagaattt cgcaaggcgg gcgctgatgt cattacggta cattatgagg catgccctca
    11221 cctgcatcgc actgtacaac aaattaagca gacgggtgca agagccggtg ttgcattgaa
    11281 ccctcataca cctgttgagg tattgcagga catcatcatc gaccttgaca tagtactcat
    11341 catgtctgta aatccgggat tcggcgggca atcgtttata gaacaaacct ataataaggt
    11401 aaaaagagtt aaaagcatga tattggctac cggctcaact gcattgatag aagtagatgg
    11461 aggcgtaggc ttgggcaata caaaacggct tgtggaatgt ggcgctgatg tacttgtagc
    11521 cggcaatgct gtatttaagg ccgaaaaccc tgctcaaacc attcaccagt taaaaaacct
    11581 gacggacaaa acatatcttg tctagtatcc gttcaaccca ttggcttaat aagacagtat
    11641 gcagatattc ctgaagcgtt ttcttttcat tgtaatggtt cttaattgtg ttgccgcatt
    11701 cgcgcaatac aaaactgtgg tcacaggtcg ggtagcagac cttgagacaa accaaggtat
    11761 agaatttgta aacatacgca tcgacggtac tacacgtggt acagaatcag acgaagacgg
    11821 aaacttcgaa atagtagtaa atacgaatga aaaagcgaat ctgattttca cacgtatagg
    11881 ttataaacaa tatacccaca gcattccttc attttctcag ggacaattga agcttgacat
    11941 aaaaatggtg ccagcagact ccaaaatgga agtggtgatt acggaagaaa aaattatctc
    12001 cgggggcatg gtcagacaaa atgttgaaga actaaagtta ttgccaagtg ccagtggcaa
    12061 tctggaaagt gcattgccca gtatagcgct cggtacatct tccggtacag ggggcgaact
    12121 tagtgcccaa tacaatgtac gtggaggcaa ctacgacgaa aatctggttt atatcaatga
    12181 ctttgaaata ttcagaccac aactcatccg tacatcgcag caagaaggtc ttactttccc
    12241 gaatatagac cttattcgcg atctgtcttt ttcttccggt ggattccagg caaaatatgg
    12301 tgacaaactt tcttcggtgt tagacattaa ttataaaagg cctgaaaaaa taaaagcctc
    12361 tgtatccggg agttttttgg gtggctcagc acatcttgag ggctctaggc caatacaccg
    12421 cgatacttac cgaaaatttc gttaccttgt aggcgcacgt tataaaactt caaaatatct
    12481 gctcaattcg ctggaagtgg agggtgagta cgtgccgaca tataccgata ttcaaacatt
    12541 ccttacttac gatataacga gaagtcttca attgggcatt attggcaact acaacaagag
    12601 caattattat tttaaacccg aaagcagtag ttcgggtgca ggcttggtca attttgcact
    12661 tcgtctgagc actgcgtatg aaggacagga agcagacgat tttgcacaaa tgatgggtgg
    12721 attatcactt acattcgtac cagatacgaa gaaaaatcct tatttcatca aactcttggc
    12781 cgctgcgcac cgtggctacg aagacgaaac atacgacatc ctcggatact attcccttgg
    12841 tcaggtggag accaatcttg gttcggacaa ggcaggccag gtagtagccg tactcggtga
    12901 aggcacgcaa cacaattacg ctcgcaacca cctcatctct acggtcaaaa atctcgaact
    12961 gcgtggcgga cacgattttc aaggaaagaa tgaagatggt aaatcggcca acaacttcct
    13021 cgagtggagc ttaaaggcac aatctgaaga aataagcgac aaactaaatg aatgggaaag
    13081 gctcgactca gccggttatt cactcaacta cagtctggaa aatctgtacc tgaagtcggt
    13141 attgaaaacc aaaaaccaga ttaattcgat aaaatattct gcttttatcc aaaacactta
    13201 taccatcaag accgatgata aggcagaatg gaaactcaac ctcggagtac gggcaatgta
    13261 ccgcgacctc aacagcgagc tgcttgtcgg tccacgagca caagtgcaat tcaggccatt
    13321 aagctggcac aacgactggg cattccggtt atccggtgga tattattatc aaccggcttt
    13381 ttatcgtgaa atgcgaagac ccaacggcac tgtaaatacc ggactactca gccaaaaatc
    13441 ggctcagata gtaggcggta tatcaagaca aataaactgg acccggattt cagacaaacc
    13501 ttttacgctt atagcagaaa tgtattataa aaagctttgg gacgtggttt cgtacgaagt
    13561 agataatgta cgcataaggt attcgggtga aaataatgca acaggctacg ttacaggatt
    13621 cgatctcagg cttaacggcg aatttgttcc aaatgcagag tcatggatta atctgtcctt
    13681 cctcagagca cgcgaaaacc tggatggcgt acagcacaaa atacgtgagg ttgggcaagc
    13741 agaaggcaag gacgtagcag atgtaccccg ccctaccgac cagttgttca atgtcaatat
    13801 atttttccag gattatctcc cacgtaacga aaactttaaa gtacacatta atctggcagt
    13861 aggttcgggg ctaccattcg gcatcaaaga caataatcag gtgtatagaa atacctaccg
    13921 gtacagtgca tatcaccgtg cagatatagg ttttagcttg ttgttgtggg acaagaaatg
    13981 gaaagatcgc aagcctcatc attttctgcg attcagtgaa aatacatggg taagtctaga
    14041 aattttcaac ctgctgaacg tacaaaatac agcctccaac aactggataa aaactgtatt
    14101 caacacacaa tacgccattc cgaaccgact tacgagtcgc agaattaacc ttaggctcag
    14161 aatggatttc taacaatttc acataaaaaa gaagcatgaa aaatcttttt caaaaagaaa
    14221 tattagaaga agtacttacc aggatagaac atcttactcc ggatacacca gcacgatggg
    14281 gcgtaatgaa tgttgcacaa atgctggctc attgtgccga atcagttgat atggcaacag
    14341 gcactaacaa acccacccgg atgcttttag gccggatttt cggaaaatta ttccgccctg
    14401 tgtacagcaa tgctatccca tttatgaaaa acctgccaac agccaaaagt ttaaggatta
    14461 gagatgaaag agattttaat aacgaaaaag aaaagctgat tacaaaacta aaacaattca
    14521 gcaaaggagg tgaagcagtc tgtacttctt ttccgcatcc gctactgggg cggcttactc
    14581 ctaatgaatg gagtgtaggc acatacaaac acctcgacca ccatctcaga cagtttggag
    14641 tttgaaaaat ggatgtcgaa tatcatttag aatagaaagg caggaattac ttcgcgttgg
    14701 taaatccagt ttgctttggt aaaattcgtt gtatctttgc aaaactatat tgcatgtctg
    14761 ttgcagacaa agatatttca acaaaccaga aaaccatggt agatatcatc agatttttta
    14821 aattcggatt agtaggctct gtaggcattc tgatagattt tagtctgacc tggttatttc
    14881 gagaccgatt gaaatggaat caatatatag ctaacattat tggattttgt gtggctgcga
    14941 gcagcaattt tgtattgaat aaaattttta cctttcagga tcaggatcac acaataatta
    15001 cgcagtacct tacatttttt atgatttcgt gtattggatt gataatgaac tcattatttt
    15061 tactgatgct acagaagaaa accaatctca atttttattt tagcaagatt ctggtaaccg
    15121 gtttgctctt tctttggaat tatgctgcca atagtatata cactttcaat accaccacat
    15181 gagtttaagc caaaaaacca tactactgat aattattgct actgggctgc gaataatatt
    15241 tgcaggaagc atcgaattgg gcaatgatga agtgtactac ataacttatg ctcaaaagtt
    15301 gcagtggaat tatttcgatc atccaccatt ggttgggtta ttgataaaga tcaccagcct
    15361 caacttgtgg cttactaacg agatatttat ccgcctcggc ccgatattat tggccgccgt
    15421 tgatacatgg cttatatttc gcataggtac tcatatcaaa aatgaaaaag ccggatacct
    15481 tgcagcatta ttcttcacag catcacctta cacttccatc ataagcggtc tgttcattat
    15541 gccggatgct ccgatgttgt ttttctggtt gctgagtatc tggttattag ttcgcgtatt
    15601 cgaccccaac atatcaaaac gtcaatcgaa catctcattg ttgctatttg ggctcacagc
    15661 tggtctagct ataatgggta aagtacatgc tatttttcta tgggtcggtc taggcattta
    15721 tttattattg tacaaccgaa ggatgctgct aaatccggtt ctttacctgg ccggtgtgat
    15781 aagtatggct atcatttcgc cgatatattt ttggaatgcc caaaacgact tcattaccta
    15841 taagtttcag ggcagtagag tcacagtcaa cagcggattc cgtgccgaca gtttgcttac
    15901 ggagttgttc ggtggcattt tgtacaacaa tccggtcaac tacgttctga tccttattgc
    15961 actaattgcc atttttcgaa aaaaacgccc atcggtattc ccctacagaa atctgctttt
    16021 gtgcctgagt ctgccactta tcggtatttt aatatttgtg tcttgtttcc gttccacatt
    16081 accgcactgg aatggtccgg gctttgtagt tcttagcatc gttgcttcag catggctggc
    16141 agaaacacat tttacgaaat acaataaatg gcttacaact gctaatattg tccttttact
    16201 cattgcgata gtggctgttc tgggtattaa tttttatccc ggcacgccgg gcaataaaga
    16261 ctatgcaaaa ttgggagaag acgacctgac actggaaatg tacggctgga aacagttcaa
    16321 tcaatcattc aggcaaatgt atgaagacga cacccgctcc ggaaaaatga agaagaattc
    16381 ggccattgct acacacaaat ggtttccggg tggccatctc aatttttatg tagctctgcc
    16441 agacagcatt acccttatcg ggcttggcac attaaatgac atccaccatt tctactggct
    16501 caatgaagtg cgaccaaaat taaagaaagg agatgatgca tatatcatag taccgtccaa
    16561 tcttaactat tctgtgcgtg tagatgacac ctacggtcaa atgtttaatg ctattgaaaa
    16621 accgagcatc atcgaccagt tgcgaaatgg caaaccggca aggtatttct ctgtatatcg
    16681 cgcgcacggc ttcaggccac ccgaagcggt tggttactga tgtgctgagc cgggcgatgg
    16741 gtgtttttcc atttttttat gaatttcatt tagcaggttc aattgtcttg cttgtgtttc
    16801 gaacaaggtt tttatttgtt cttcaagcag tagatccact ttctgatgca gcctaagtat
    16861 ttccatttcg gctttcagat tgatcaggta atcattttct gctcttattc ggtctttttc
    16921 ttcttgtcgg ttttggctca tcataatcac tggagcttga atg
//
LOCUS       DAOQQY010000027        16718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1069_length_16718_cov_5.937166, whole genome shotgun sequence.
ACCESSION   DAOQQY010000027 DAOQQY010000000
VERSION     DAOQQY010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16718
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1069_length_16718_cov_5.937166"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="PLE23_02830"
     CDS             <1..644
                     /locus_tag="PLE23_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HMX82230.1"
                     /translation="LIAYNKITKDRYLPTGPELSQSAQLYDISGDKMQLILDFPTIGE
                     PHYAQAAPADMIRNNGQYKIYKIDENKHPYVTKGEKDSKVVREGNKVHVYITAIRSHF
                     SPDNIEGVRMGDEVYFHVTNLEQDWDVPHGFAVKGANNGELLIMPGETQTLKWVPDRP
                     GMFPIYCTDFCSALHQEMQGYMRVSPAGSKTPLMYSLGAQSPDAEAVTEPAKK"
     gene            745..1758
                     /locus_tag="PLE23_02835"
     CDS             745..1758
                     /locus_tag="PLE23_02835"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HMX82231.1"
                     /translation="MTPKDNHLSIYSKVFLFISAIALVVSIFVPIWRIDLDAPQYPEG
                     LRMLISANGLGGDVEIINGLNHYIGMKTLHNEDFIEFTVLPYIIGFFALFALLTVFVN
                     RKKVLYILFSAFVLFGILAMYDFWRWEYEYGHDLDPNAAIVVPGMAYQPPLIGFKQLL
                     NFGAYSIPDFGGWLFIGAGVLMLLSLLIETNMLKLKKMNPLVVLLAMWFLPACGQKNP
                     EPIKINKDNCAFCKMTIADKKFGTELITPKNRVYKFDDVKCMVGYVKENPAAVGSRFF
                     VPDYLEPNALISVESASFIKGGTIESPMAGNIAAFADKTKAEKHAKDTGAKILNWNEI
                     MKK"
     gene            1765..2997
                     /locus_tag="PLE23_02840"
     CDS             1765..2997
                     /locus_tag="PLE23_02840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HMX82232.1"
                     /translation="MKYFVALTLVAFSVLSHAKTLQVGKNFPYKTIKEALAASENSDT
                     LIINPGLYREGNIVIEKAVSIIGVNLPIIDGEHKFEVISVKSSNVLVKGLKVINSGIA
                     TLEDPCGIKVYQSENVTIRENVLIDNFFAIYLMSSINCTVEQNYVSASATEEQAIGNG
                     IHCWKSKDLKIINNFIKGHRDGIYFEFVTHSVIWRNISKRNLRYGLHFMFSNDDSYIG
                     NVFEENGAGVAVMYTKHVKMFNNVFRENWGDAAYGLLLKEISDSQIQNNKFTNNTTGI
                     LIEGSSRIQMSQSELKSNGWGIKISASCEDINLTQNNFLSNTFDIATNGSLVLNNFDG
                     NYWDKYEGYDLNRDNVGDVPYHPLSLFSVIVERMPSAMLLFRSFMITLLDKSEKILPS
                     LTPENFIDNKPFMKPLAI"
     gene            2994..3707
                     /locus_tag="PLE23_02845"
     CDS             2994..3707
                     /locus_tag="PLE23_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX82233.1"
                     /translation="MIEINSLSKSFGKLDVLKNVKLTLNPNQSVALIGPNGCGKTTLI
                     KSILGMVLPDSGEIKFDGHKIRNQFQYRNEIGYMPQIGHYPENMTIGHIIEIMKEIRN
                     SSKELDEDLLHAFKLKNMFEKKMRTLSGGTTQKVSATLAFLFDPKVLILDEPTAGLDP
                     LASEILKEKIIAEKQKGKIIMITSHLLSELDDLINGLIYMQDGQILLDSPIEEVKVNT
                     GQDTVSKAISHILKTMQHA"
     gene            3700..4467
                     /locus_tag="PLE23_02850"
     CDS             3700..4467
                     /locus_tag="PLE23_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMX82234.1"
                     /translation="MLRILKYVIRDILNNKIVIAYALLLAVLSWSMFSLEDSSSKGVL
                     SLLNIILLNVPLVSVIFSTIYIYNSGEFIELLLSQPIKRSKIWSSFYLGLCISLIVAF
                     LIGAGIPILVYSPDSTGLMMIVVGVLITAIFVSLAFFSAVFTRDKAKGIGFAIMLWLF
                     FALLFDGLVLFLLFQFADYPIEKAMVGITALSPIDLSRIMVLLHLDVSAMLGYTGAIF
                     KEFFGTLTGMIVTFSLLGLWVLLPFWFSLWRFKKMDL"
     gene            4686..5288
                     /locus_tag="PLE23_02855"
     CDS             4686..5288
                     /locus_tag="PLE23_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMX82235.1"
                     /translation="MAGPDITFRYIDGDLYKDEIGKFNYRFGLNYTFQISNRIVLEVG
                     ARYASIGYQTAKMDIQWGSEHNGNGGYEPDPNLPHNFRNSSDYRFIEFPLSAKYILKK
                     GKFTPYIAAGISPNVYLNTRSVTIIEAKTEISVSKTQNINKLNLSAHAGFGIDYQWKK
                     SLEIYMQPSFRYYLKSISSGPIKEYLYSAGIELGIRKLFR"
     gene            complement(5497..6054)
                     /locus_tag="PLE23_02860"
     CDS             complement(5497..6054)
                     /locus_tag="PLE23_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HMX82236.1"
                     /translation="MKSLINLFFVLFVFAAVSCATETTESTTTASAPEASAATVGQSG
                     VQDETSNPNIVQVAVGNKDFSTLVTAVKAAGLVDALSNAGPFTVFAPTNAAFDKLPKG
                     TVEGLLKPEKKDDLDNILSYHTYVGVLKPEILQDGATYEMVNGGKVKITKKDGKTFVN
                     DVEIAASVPTSNGIIHVIGDVLLPK"
     gene            complement(6135..7349)
                     /locus_tag="PLE23_02865"
     CDS             complement(6135..7349)
                     /locus_tag="PLE23_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82237.1"
                     /translation="MDKRFKSWINLSLLNLTIVACIGVLMRYKIGFDFPYLEQKNILH
                     AHAYFAFTGWVTHLLYTFIYYHYKQEFTQKGLRYAVMLIRTNLVASYGILITYFVFGY
                     SYQASFFVAVTILASYFFVALFLSQLRYFDHNPGTKWLAAGLIFYACASGGAIYNAYM
                     LSAGTFSQKIFLACQYIYLHFQFNGWFLLSCIGLFIGLLSRKGIAFDDNNIFRMIAYM
                     CAPTLFLSTLWMKMPSWLYWGTVAATILQCVGWAMVVKFAWDNRLLIKEKFTRNTYSL
                     FLLAAMALSIKMILQLGSTHPQISKLAYGFRPIVIAYIHLVVLGVITIFLFGYAYAMK
                     IIPSSRLSHAGILLFVGGIFLNEFVLMIQGVASFSYFAVPHINQVLVFVSLIMLAGIV
                     MVLIARKEIDQQ"
     gene            complement(7351..7944)
                     /locus_tag="PLE23_02870"
     CDS             complement(7351..7944)
                     /locus_tag="PLE23_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMX82238.1"
                     /translation="MLIDPEILTKAGGILKWYKKNKRIFLENEMPENYYQIVTGLVKL
                     ANVNDRGKEFIQGIFRPGECFGEPPLFLGKEYFASACAMVDCEIYELPRDAFFKILKK
                     HPEIEMMFLKNFANRIYNRSLASRNIISNDPVQRITGFFDQYKQGTNSSTERMKIPFT
                     RQEIANFTGLRVETVIRTLIRMKEENQVEIADRKIYY"
     gene            8199..8828
                     /locus_tag="PLE23_02875"
     CDS             8199..8828
                     /locus_tag="PLE23_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMX82239.1"
                     /translation="MIEKLTSLFEPGLVAELEATGNLRSFHEGDIIMDYGKYINSMPV
                     ILSGNVRVLTQDEEGNEILLYYLSSNESCAMAYTCCMDAKQSEVKAVAEDDVTLVTIP
                     QKVMDEWLCKYPSWRTYIMHSFTARFNELLASFESIAFKKLDERLVKYLTDRQKISGS
                     SIVKASHQQIADDLATSRVVISRLLKHLENEKKLILYRNEIKLLKAINM"
     gene            9043..10263
                     /locus_tag="PLE23_02880"
     CDS             9043..10263
                     /locus_tag="PLE23_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HMX82240.1"
                     /translation="MNQKLLDKINAFAIPDDLRTAGVYPYFRVIESEQDTMVNIGGNP
                     VLMFGSNSYLGLTNHPRIKEAAKKAIDIYGTGCAGSRFLNGTLKIHIELEELLCDYLK
                     REAVLLYSTGFQANLGAIAPLAGRHDYILIDERVHASIIDATRLSYAKVLKFRHNDMA
                     DLDDIMSRLNTDSNKFIVVDGIFSMEGDIANLPEMVKIAKKYDANLIVDDAHSVGVIG
                     KEGIGTGSHFDLHQEVDMQIGTFSKSLASLGGFIASSADVIDYLKHTSRSLIFSASMT
                     PSSAASVMEAIHIIREEPEHIENLWKNTHLSMKIFNEMGFEIGHTESPIIPVYIRDNM
                     KTFEFSRLCFQEGLFINPVVSPAVPSEESLIRFSLMANHTYEQIERAADIMYRNAVKL
                     GVIDARKASVLINK"
     gene            10304..11428
                     /locus_tag="PLE23_02885"
     CDS             10304..11428
                     /locus_tag="PLE23_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82241.1"
                     /translation="MVEIIEVKSKKQLTQFIDFPHELYKNDPNYVPMLYMEQEALLNK
                     AKSPFLKHSEAVYYLAVKEGKFVGRIAAIKNNNHIRFTGKQEGFFGFFDVINDYEAAN
                     ALLSKAEAWLKTKGLNKIFGPANFSTNETVGLLIENYEEPPFIMNTYNAPYYIDILEK
                     YGFKKSTDLLCYELITAEMQDEIIELGERLEGRLAEKGITIRKINMKNYAEEINNFLP
                     VYNESWIENTGFVPMTDDEVRQIGKDLKPIVDPDFVYFAIKDGKMIGVSLTVPNINEI
                     QINMKRGRLLPFGWWKILFGLKSIKSVRVLALGTLKEYRRLGIDVCFYVRNIKTARAK
                     GIVRAEASWILENNTMMNRSLEHINGKVYRKYRIYEKPVS"
     gene            11428..12414
                     /locus_tag="PLE23_02890"
     CDS             11428..12414
                     /locus_tag="PLE23_02890"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMX82242.1"
                     /translation="MKTLLITGANGFIGSTLVECAIQNNYKVTAAIRKGSDLNYLKDF
                     DIDYLEIDYNNKEDLNEKFSRLPAFDYIIHNAGITKAKDEAGYHKVNVGNTKLLVETL
                     IETGKVPHKFLFVSSLAAVGPVTYGKTIQDDTPGNPVTWYGRSKLVAENYFTTLNDFP
                     WVIVRPTAVYGPKDKDIFVFIKMICSGFNLSVGYRDQELSFIHSEDLVALMLEILRNG
                     QTGKKYIGYDLRQYTSEDLSSAIKEALHKKAIRLRLPVGIISMVAFLSEKIGKLSGKI
                     PALNIDKVNELKAQCWWCNSENVKNLSNWQPKYDLYSGMKQTIAWYKDKGWL"
     gene            12562..13035
                     /locus_tag="PLE23_02895"
     CDS             12562..13035
                     /locus_tag="PLE23_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HMX82243.1"
                     /translation="MTNITEGTAVKGFLRLVSQYGSTSLFATVLDFGVFEIALKYFAA
                     SAVIATLFGRAVGSITSFLIHRHWVFNHVTGLDIKVLLTKYIIGIIIGTILNLGGVWL
                     LNEVVGFGPWPSRIITSTTVWFIVFTYNRLWVFSKGRNITTQLANSSVVHEEEHN"
     gene            13126..14868
                     /locus_tag="PLE23_02900"
     CDS             13126..14868
                     /locus_tag="PLE23_02900"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMX82244.1"
                     /translation="MLGRIRYIFIFLLLFLSWVVVSQNTSSVQGTVTDTVTGEGIPYA
                     SIRISGQVEGITSDINGKFYIKLAQLPSTITVSYIGYETKVVQILQNTIQPLQIKLQP
                     SAVEISEVEVRVQKYKNKGNPAVELIKKVLGNKEKNRPEAFDYFQYKKYEQVELAFNN
                     IGDNTRNSLLFRKMKFIFDYADTNRTNGKVFLPFFFRESLSDVYYRRSPNAEIEYIRS
                     EYNTVVPELVDNLGVANFLSNLSQSVNIYDNSINLVTVDFVSPLSPISPNIYRFYILD
                     TGVVNDTKLVHLYFAPRNKSDKAFIGNMWIALDSTYSVRKIDLGIPKEINLNWVNEMQ
                     ISQEFDWVSTVITINGKMITSRGLMIKSNEYFMDFAVLDNKKAKSLLGAKRIKYQDLQ
                     INRPIPDSIFKENIKIFRTVSTDTMSQAYLQQNRPDTLTRSQQNIIAMVDSLRNHKPF
                     KNLIKLGKVFMHGYADVGPVSFGPVHNLYSFNSVEGTRLRVGLRTNEKFSKRLLLEGY
                     AAYGLKDEHWKGKASARYSLTNADVLKFPYNQLRVWYENEVKVPGQELNFIQPDNFLL
                     SFKRGLTTKWCTRK"
     gene            14850..15710
                     /locus_tag="PLE23_02905"
     CDS             14850..15710
                     /locus_tag="PLE23_02905"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMX82245.1"
                     /translation="MVYQEVVGLEYTKESQSGFSYALGVRTSRQTPAGQLKFEYLQDG
                     QLLTKHSMQLTQVGLTLRYAPNEEFYQTLYSRISLSNKYPKITLSYLLAAKDILGGEY
                     SYHSVNLRFQKLFYISPLGYSKVHIDAGAVFGKVPFLLLKIHNANQSYSYQFDAYNLM
                     NYLEFVSDRYASVHIFHNFQGILFNRLPLIKKLKLREVASFKAVFGGLSKNNNPFLNN
                     NAGLFVFPMDAQGRQTTYTLDKEPYMEASFGISNLFRFFRLDYVMRLNYLSHPDISKW
                     GIRFNFVFQF"
     gene            15814..16197
                     /gene="trxA"
                     /locus_tag="PLE23_02910"
     CDS             15814..16197
                     /gene="trxA"
                     /locus_tag="PLE23_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMX82246.1"
                     /translation="MAKKERHTIQLTKAEFLEKVMDYEKNQTAWVYKGDKPCVIDFYA
                     DWCGPCKMVAPVLEELAEEYKDHIYIYKVDTEAEMELSAVFGIRSIPTIIFCPMGEQP
                     QAVNGALPKHNLEQIIKELLLKEKL"
BASE COUNT         5286 a         3180 c         3409 g         4843 t
ORIGIN      
        1 atctgatagc ttataacaaa attacaaaag accgttatct gcctacaggt ccggagcttt
       61 ctcaaagtgc acagttgtat gacatttcgg gcgataaaat gcagcttata cttgacttcc
      121 cgactatcgg tgagcctcac tatgcacaag cagcaccagc cgacatgatt agaaacaacg
      181 gacagtacaa gatttataaa atcgatgaga acaagcaccc ttatgttacc aaaggagaga
      241 aagactctaa ggtagtgaga gaaggaaata aggtgcatgt ttatattact gccatcagat
      301 cgcacttttc accagacaat atcgaaggtg taagaatggg tgacgaagta tatttccacg
      361 ttaccaacct tgagcaagac tgggatgttc cgcacggttt tgctgtcaaa ggtgccaaca
      421 atggcgagct cctcatcatg ccaggcgaaa cacagacact caagtgggta ccggacagac
      481 cgggtatgtt cccgatttac tgtaccgact tctgtagtgc acttcaccag gaaatgcaag
      541 gctacatgcg agtttcaccg gccggtagca aaacgccgct tatgtactca cttggtgctc
      601 aatcaccaga tgcagaggca gttactgaac cagccaagaa atagtaaaga agggaatctg
      661 ttcccataat tctaaaaatc aggagcagaa ttttcgggtt ctgctccttt ttttacatcc
      721 aataaacaca aaattttaaa aatcatgaca cctaaagata atcacttgag tatctactct
      781 aaggtattcc ttttcatctc tgctatcgca ctcgtagttt ctatctttgt tcctatctgg
      841 cggatagatc tcgacgcacc tcagtatccg gaaggattgc gcatgttaat ttctgccaac
      901 ggtctcggcg gagatgtgga gattatcaat ggtttgaacc actatatcgg tatgaagaca
      961 ctgcacaatg aagacttcat cgaatttaca gtgttaccat atatcatcgg tttttttgca
     1021 ctttttgctt tattgacagt gtttgttaac agaaaaaaag tcctttacat tctgttttcg
     1081 gcctttgtgc tttttggcat actcgccatg tacgatttct ggcgctggga atacgaatac
     1141 ggacatgacc ttgaccccaa tgctgccata gtagtaccag gtatggctta ccaaccacca
     1201 ttgattggtt tcaaacaatt gcttaacttt ggggcttatt ctattcctga tttcggtgga
     1261 tggcttttca tcggtgcagg agtacttatg ttattgtcat tattaattga aacaaatatg
     1321 cttaaactaa aaaaaatgaa cccactggtt gttttgctgg ctatgtggtt cttacctgct
     1381 tgtggtcaga aaaatcctga acctattaaa ataaataaag ataattgtgc attctgtaaa
     1441 atgaccattg cagacaaaaa attcggcacc gaacttatta ctccaaaaaa cagagtttat
     1501 aaatttgacg atgttaaatg tatggtaggc tacgttaagg aaaatcctgc tgctgtcggc
     1561 tcaagatttt ttgtccctga ttaccttgaa ccaaatgcgc tgatatctgt tgaatctgcc
     1621 agttttatta aaggtggaac gattgaaagc cctatggcag gcaatattgc tgcatttgcc
     1681 gataaaacaa aagccgagaa gcatgccaaa gatacaggtg cgaagattct gaactggaac
     1741 gaaataatga aaaaataaac cgacatgaag tattttgttg cgttgaccct cgtggcattc
     1801 tccgttttgt cacatgctaa aacgttgcaa gtaggaaaga attttccgta taaaacaata
     1861 aaggaagctc tagcagcaag tgaaaattca gacacactga ttatcaatcc aggtttgtac
     1921 cgagaaggta acatcgttat tgaaaaagcc gtcagcataa ttggtgtcaa tctacccatc
     1981 atagatggtg agcacaagtt cgaggtgatc tctgtcaaat caagtaatgt actggtcaaa
     2041 ggacttaaag tcattaattc cggtatcgct acacttgaag acccttgtgg tatcaaagta
     2101 tatcaatcgg agaatgtgac aattcgtgaa aatgtcctca tcgataattt ctttgccata
     2161 tacctgatga gcagtatcaa ttgtactgtt gagcaaaact atgtaagtgc gagtgccacc
     2221 gaagagcagg ctatcggcaa tggtatccat tgctggaaaa gcaaagacct gaagataatt
     2281 aacaatttta taaaaggaca ccgcgacggt atctattttg agtttgtaac gcattctgtc
     2341 atttggcgga atatctctaa gcgcaatttg cgttatggtt tgcattttat gttctcaaac
     2401 gatgattcgt acattggcaa tgtatttgaa gaaaatggcg ccggtgtagc tgttatgtac
     2461 acaaaacatg taaagatgtt taataacgta ttccgtgaaa attggggtga cgctgcttac
     2521 ggattattgt tgaaagagat atccgacagc caaattcaga ataataaatt tacaaacaac
     2581 actaccggta ttctaatcga aggttcaagt cgcatacaga tgagtcagag cgaactgaaa
     2641 tcaaacgggt ggggaataaa gataagtgcc agttgcgaag atatcaactt gacacagaat
     2701 aactttctga gcaatacatt cgacatagcc accaacggtt ctttggtgtt aaataatttt
     2761 gatggcaatt actgggacaa gtacgaaggc tacgacctta atagagataa tgtaggcgat
     2821 gtaccttatc atccgctgag ccttttttca gtaatagtcg aaagaatgcc ttcagccatg
     2881 cttctgttca ggagttttat gattacattg ctcgacaaat cggaaaaaat acttccaagc
     2941 cttacaccgg aaaattttat tgataacaaa ccattcatga aaccacttgc tatatgatcg
     3001 aaataaacag cctctcaaag agttttggga aactggatgt attaaaaaat gtcaaactca
     3061 ccttgaaccc caatcaaagt gtagcgctca tcgggcctaa tggctgtggt aaaaccacac
     3121 ttattaaaag tattctggga atggtactac ctgactcggg cgaaatcaaa tttgacggac
     3181 acaagataag gaatcagttc caatatcgca atgaaattgg ctacatgcca cagataggcc
     3241 attatccgga gaacatgacc attggccaca tcatcgaaat catgaaggag attcgaaatt
     3301 catcgaaaga gctggatgaa gatttgctcc atgctttcaa actgaaaaat atgtttgaaa
     3361 aaaaaatgcg tacactttcc ggtggtacta ctcagaaagt aagtgctacg ctcgcatttt
     3421 tgtttgaccc gaaagttctc atactggatg agcctactgc tggcctcgat cccttggcgt
     3481 ctgaaattct caaagaaaaa attattgcag agaaacagaa gggcaaaatc atcatgatta
     3541 cctcacacct gctcagcgaa ctcgacgatc tgattaacgg acttatctac atgcaagacg
     3601 gtcagattct gctggatagc ccgattgaag aagtcaaggt gaataccggg caggatacag
     3661 tgtcgaaagc catttctcac atcttgaaaa ccatgcaaca tgcttaggat attaaaatat
     3721 gtaatacgag atatactgaa taacaagatt gtcatagcct atgccttgtt attggccgtt
     3781 ttgtcctgga gtatgttcag tctggaagac agcagcagca aaggcgtgtt gtcactattg
     3841 aatattatcc tgctcaatgt gccgcttgtt tctgttatct tttcgaccat ttatatctac
     3901 aatagcggag aatttataga gcttctgctt agccagccta ttaagcgaag caaaatctgg
     3961 tcgagttttt atctcggatt gtgtatatca ttgattgttg cattcttaat aggtgcagga
     4021 ataccgattc tggtgtattc accggattca acgggcttga tgatgattgt agtaggagtt
     4081 ctcattactg cgattttcgt ttcgttggca tttttcagtg ccgtatttac acgcgataaa
     4141 gcaaaaggta tcggttttgc tattatgtta tggttgtttt ttgccctgtt atttgacgga
     4201 ttggtactat tcctgctttt ccagtttgcc gattatccta tcgaaaaagc aatggtaggg
     4261 ataactgcgc tgagcccgat tgacttgtca agaatcatgg tgctgctcca tttggatgtt
     4321 tctgcgatgc tcggatatac aggtgctatt ttcaaagaat tcttcggcac acttaccgga
     4381 atgattgtca cattctcatt gttgggttta tgggtgctcc tgcctttttg gttttcactt
     4441 tggcgtttta agaaaatgga tttgtaattc agaatctttg attggcttca aagctaatga
     4501 tatgatggct tttgagtgag tttaggctga tatatgtttg attagccaat ttttagtatt
     4561 aattttgtct ggtaatcata aaacaaaacg gatacaagat gaactgcaca aaattattag
     4621 ctgtcctaat tttacttacc acccattttt cagtattctg ccagaagcag tcctcccttg
     4681 gaattatggc cggacctgat attaccttca ggtatataga tggcgatctt tataaagatg
     4741 aaattggtaa gtttaattat agatttggac tcaactacac atttcagata agcaatagaa
     4801 ttgtattgga agtcggcgca cgctatgcaa gcattggcta ccagacagct aaaatggata
     4861 ttcaatgggg atcggagcat aatggtaatg gcggatatga accagatccg aacctgcccc
     4921 ataatttccg caatagctct gattacagat ttatagagtt tcccctgagc gcaaaatata
     4981 tactgaagaa gggaaaattc accccatata ttgctgccgg catttctcct aatgtatatc
     5041 tcaataccag gtctgtaacc atcatagaag caaaaacaga aatcagtgta tcaaagaccc
     5101 aaaatatcaa taaactaaac ctttctgccc atgccggatt tgggatagat tatcaatgga
     5161 aaaagagcct ggaaatttat atgcagcctt catttcgtta ttatttgaaa agtataagca
     5221 gcggccctat aaaagaatat ttgtatagtg caggcataga acttggtata aggaaactat
     5281 tcagataaca actgaaatgg aaagctaaaa tccgtcactc tgttttatat agcttttcat
     5341 acagcaggat gttacagttc cgttgatgat aacggcgttt tttactgctc aatcatctct
     5401 gacagtcaga gtttaaagct aggctacttt tttatagagt aaaaaaaggc acctgaaccc
     5461 aatcagatgc cttggttatt taagactatg aaaaaattat ttaggaagta gaacatcacc
     5521 aataacatgg atgatgccat tagaggtagg cacagatgca gctatttcca catcgttgac
     5581 gaatgttttt ccatcttttt tagtgatttt tacttttcca ccgttaacca tttcgtacgt
     5641 agcaccatcc tgcaatattt caggtttcag tacaccaaca taggtgtgat agctaaggat
     5701 gttgtcaaga tcatctttct tttcaggttt caaaagtccc tcaacagtac ccttaggtag
     5761 tttgtcaaaa gcagcattgg taggagcgaa tacggtgaaa ggccccgcat tgctgagtgc
     5821 atccactaag ccagcagctt tgactgcagt cacgagtgtc gaaaagtctt tattgccaac
     5881 tgccacctga acgatgttag ggtttgatgt ttcgtcctga acacccgatt gacctaccgt
     5941 ggcggcagat gcttccggtg ctgatgcggt ggttgttgat tctgtagttt cagttgcaca
     6001 cgaaacagca gcaaacacaa ataatacaaa aaacagatta attaatgact tcataatgtg
     6061 tgattttatt taccacaaag ctcgataaat tttggcacac attttatgac ttaaatcata
     6121 aagataattg acttttattg ttggtctatt tcttttcttg ctatcaaaac catgactata
     6181 cctgccagca tgatgagaga aacaaataca agtacttgat tgatatgtgg cactgcgaaa
     6241 taactgaaag aagccacacc ttgtatcata agtacgaact cattgagaaa tatacctcct
     6301 acaaatagca agatacctgc atgacttagt ctggatgaag gaatgatctt catagcataa
     6361 gcatagccaa ataaaaatat agtaataacg cctagtacta ccaggtgaat gtaggcaata
     6421 acaatgggac ggaagccata agccagttta cttatttgcg gatgggtcga gcctagttgc
     6481 agtatcattt taatactaag ggccatagct gccaataaaa agagactgta ggtgtttctg
     6541 gtaaattttt cctttatgag tagcctgttg tcccaggcaa attttacaac cattgcccag
     6601 ccgacacatt gcagtatagt ggctgctaca gtgccccaat ataaccagga aggcattttc
     6661 atccacaaag tgcttagaaa caaggtagga gcgcacatat aggctatcat ccggaaaata
     6721 ttgttatcat caaatgcgat accttttctg gacaacaacc caatgaacaa accgatacat
     6781 gaaagcaaaa accagccatt gaactggaag tgaaggtaga tatattggca ggcgaggaat
     6841 attttctgac tgaatgtacc ggccgacagc atatatgcat tgtaaatggc tcccccggac
     6901 gcacatgcat aaaagataag ccctgctgcc agccatttgg tacccggatt atggtcaaaa
     6961 tacctcaatt ggctgaggaa caatgctaca aaaaaatacg atgccaatat ggtgacagct
     7021 acaaaaaacg atgcctgata actgtaccca aaaacaaaat acgtaatgag tatgccgtaa
     7081 gaggccacaa gattggtacg tataagcatg acagcatacc gcaatccttt ttgggtgaat
     7141 tcttgtttgt aatgataata aataaaagta taaagcaaat gggtcaccca gcctgtaaat
     7201 gcaaaataag cgtgggcatg cagtatgttt ttctgctcaa gatatgggaa atcaaagccg
     7261 attttataac gcatcagtac accgatgcag gctacgatgg tcagattgag tagtgataaa
     7321 ttaatccagg atttgaaccg tttgtccata ttaataataa atttttctgt ctgctatttc
     7381 tacctgattt tcttctttca tacgtatgag tgtgcgaatc actgtctcta cccgcaggcc
     7441 ggtaaagttg gcgatctcct gtcgggtgaa aggaatcttc atcctttcag ttgatgagtt
     7501 ggttccttgt ttgtactgat caaaaaagcc ggtaatgcgt tgaaccggat cgttactgat
     7561 aatgtttcta ctggccaacg accggttgta aatacggttg gcaaaatttt tcaaaaacat
     7621 catttctatt tcaggatgtt tcttcagtat tttaaaaaag gcatctcttg gcagttcata
     7681 aatttcgcaa tcaaccattg cacaggcaga tgcaaaatat tctttaccaa gaaaaagtgg
     7741 tggttcgcca aaacattctc ccggtctgaa aattccctga ataaattcct tgccacggtc
     7801 gtttacgttt gcgagtttta caagtccggt gactatctga taataatttt ccggcatttc
     7861 attttcgaga aatatccgtt tatttttctt ataccactta agaatgccgc ccgctttggt
     7921 caatatttct gggtcaatca gcatagttta acaatagtct tggttcagct gttctggttt
     7981 gtttattgaa tgatggattc cggatactgc gtatgttttt atgtttgaac aattgctatt
     8041 gggcatacca acaaggtatc ttgttttctg ataaaaattt tgcccaaata tactggtttt
     8101 tttcatgcgg ctaatcatat ttaaccattt cttttgatag aagtgaacaa gtttgctgat
     8161 aatgtcttta ctttacgaaa aaacatttaa cacaactgat gatagaaaaa ctaacttcat
     8221 tattcgaacc cggcctcgtg gcagaactag aagcaacagg caacctgcga agctttcatg
     8281 aaggtgatat tattatggat tatggcaagt atattaattc catgcctgtg atattaagtg
     8341 gcaatgtaag ggtgctcact caggatgagg aaggaaatga aatactactg tactatcttt
     8401 cgagcaatga aagctgtgca atggcctaca cctgctgtat ggacgccaaa cagagcgaag
     8461 taaaagccgt agccgaagac gatgtaaccc tggtgacgat acctcagaag gtaatggatg
     8521 aatggctctg caagtatcct tcctggcgta cgtacatcat gcatagtttt acggcaaggt
     8581 tcaatgagtt gctcgcttct tttgaatcca tagccttcaa aaaactagac gagcgcctcg
     8641 tgaaatacct tacagaccga cagaaaatat caggttcttc aatagtgaaa gcatcacacc
     8701 aacagatagc cgatgacttg gctacttcaa gggtggtcat tagcagattg cttaaacacc
     8761 ttgagaacga aaagaaactt atattgtacc gcaacgagat caaactactg aaagctatca
     8821 atatgtgaat acatccttat gtaaaactat gtttgtaata acagtattca cagacataac
     8881 tgttaaattt tattaaaact ttaaattact atcaagtatg ttaagttttt gtatctttgc
     8941 ccattaatgt tgcggaatta tttcgtgcga aacggtaatc tggattatct tttactttaa
     9001 gtctggagag tatatttgtt aaatacatta agagtctgag gaatgaatca aaagttgttg
     9061 gataaaataa atgcattcgc gatacctgac gatttgagaa ctgccggtgt ctatccttat
     9121 ttcagagtga ttgagtctga gcaagatacc atggtgaata ttggcggtaa cccggtattg
     9181 atgttcggtt ccaattctta cttaggcctt accaatcacc caaggataaa agaagccgct
     9241 aaaaaagcaa tcgatatata tggtacaggg tgtgcaggtt caagattcct gaacggaaca
     9301 ttaaaaattc acattgagct tgaagaattg ctttgtgatt acctcaagcg tgaggccgtg
     9361 ttgttatata gtacagggtt tcaggccaat cttggggcga tagctccact tgcaggcaga
     9421 cacgactaca ttctcataga tgaaagagtt catgcttcca tcatagatgc cacccggtta
     9481 tcttatgcca aagtattgaa attcaggcat aatgatatgg ccgatctgga tgatataatg
     9541 tcaaggctca atacagactc caacaagttt atcgtagtgg atggcatatt cagtatggaa
     9601 ggtgatattg ccaatttacc tgaaatggta aaaatagcta aaaaatacga tgccaacctg
     9661 atagtagatg atgcacattc agtaggcgtt ataggtaagg aaggtatagg taccgggtca
     9721 cactttgacc ttcaccagga agtggatatg caaataggta ctttcagcaa atcgctcgct
     9781 tctcttggtg ggtttatagc ctcctcggct gatgttattg attacctcaa gcatacctca
     9841 cgttcgctca tattcagtgc cagcatgaca ccgtcttctg ccgccagcgt aatggaagct
     9901 atacacatca tcagagaaga acccgaacac atcgaaaacc tgtggaaaaa tacacatctg
     9961 tcgatgaaga tatttaatga aatgggcttt gaaatcggac ataccgaatc accgattatc
    10021 cctgtttata taagggacaa catgaagaca ttcgaatttt caaggctttg tttccaggaa
    10081 ggcctcttta ttaatcctgt agtgtcgcca gctgtgccaa gtgaagaatc tctgataagg
    10141 ttttcactta tggcgaacca tacttacgaa caaatagaac gcgctgccga tatcatgtac
    10201 cgcaatgcag taaaactggg cgtcattgat gcacgaaagg catcggtatt gattaataag
    10261 taaaatgtaa tattttctga atagctaaac aaacacatac acaatggttg agatcatcga
    10321 agtaaaatcg aaaaagcaac tcacacaatt cattgatttt ccacacgaat tgtacaagaa
    10381 tgatcccaac tatgtaccga tgctatatat ggagcaggaa gctttgctca ataaagccaa
    10441 atctcccttt ctaaaacact cagaagccgt ttattatctt gccgttaaag aaggcaaatt
    10501 cgtcggtcgg atagctgcca tcaaaaacaa taaccacata cgctttacag gcaaacagga
    10561 aggctttttt ggcttttttg atgtgataaa tgattacgag gcagccaatg cactgttgtc
    10621 aaaagccgaa gcttggctca aaacaaaagg cctgaataag atattcggcc cggccaattt
    10681 ttccactaac gaaactgtcg gtttgcttat cgagaattat gaagaaccac ctttcatcat
    10741 gaatacttat aatgcccctt attacatcga cattcttgag aaatacggtt ttaaaaagtc
    10801 aacagacttg ttgtgctatg agctgataac agcagaaatg caggacgaga ttatagaact
    10861 tggcgaacgc ttggaaggac gcctcgctga gaaaggtata accatccgca agataaacat
    10921 gaaaaattat gctgaagaaa tcaataactt tctaccggtg tacaacgaat catggataga
    10981 gaatacaggt ttcgtaccca tgacggatga cgaagtaaga cagataggca aagacctaaa
    11041 accaatcgtt gacccggatt ttgtatattt tgccatcaaa gacggaaaaa tgattggcgt
    11101 atcgctcacg gtaccgaata tcaatgaaat ccaaatcaat atgaagcggg ggcgcctgtt
    11161 gccttttgga tggtggaaaa tactttttgg actgaaaagc atcaaatctg tacgggtact
    11221 tgcactcggt acgcttaagg aataccgccg ccttggcata gatgtttgct tctatgtacg
    11281 caatataaaa actgcacgtg ctaaaggaat agtaagggct gaggcatcat ggatactcga
    11341 gaacaataca atgatgaacc gctctctcga acacatcaat ggcaaggttt acagaaaata
    11401 cagaatctac gaaaagccgg taagctgatg aaaacactac tcatcaccgg tgccaatggg
    11461 tttataggaa gtacattggt agaatgtgcc attcagaaca attataaagt aacagcagcc
    11521 ataagaaagg gtagtgacct caattatcta aaagattttg atattgacta cctcgaaata
    11581 gattacaaca ataaagagga tcttaatgaa aaatttagta ggctgcctgc ttttgattac
    11641 ataattcata atgcgggcat tacaaaagct aaagatgaag ccggctacca taaagtgaat
    11701 gtcggaaata caaaattgct cgtagaaaca ctcatcgaaa ccggcaaggt accacataaa
    11761 tttcttttcg tcagcagcct ggcagctgta ggcccggtga cttatggtaa aaccatccaa
    11821 gatgatacgc ccggtaatcc ggttacttgg tacggcagga gtaagctggt ggcagaaaat
    11881 tatttcacca cactcaatga ttttccctgg gttattgtca ggccaactgc agtatatggg
    11941 ccaaaagaca aagatatttt cgtatttatc aaaatgatat gcagtggatt taatctgtcg
    12001 gtcggatatc gcgatcagga attgagtttc atccactccg aagaccttgt agcattgatg
    12061 cttgaaattc taaggaatgg acagacagga aaaaaataca tcgggtatga tctaagacaa
    12121 tatacaagtg aagacctgag cagcgcaata aaggaagcat tgcacaaaaa ggctataaga
    12181 ttgagattgc cggtaggcat catatcaatg gttgcttttc tctcagaaaa aatagggaag
    12241 ctcagtggca aaatacctgc actcaatatt gataaagtca atgaactaaa ggctcaatgc
    12301 tggtggtgca attcagaaaa tgttaaaaat ctgagcaact ggcagccaaa atatgatttg
    12361 tattccggaa tgaaacaaac cattgcctgg tacaaagaca aaggctggct gtgagttcca
    12421 acttgtactt ttagcatcca tttgacttcg aaagtagtaa aaacacaaac aaacaaattg
    12481 agcttagttt tgtttgatga acgttacaaa taacaaatca gttagtcggt tgtaggcaaa
    12541 ttaggtacca aaacctttag catgacaaat ataacggaag ggacagcagt gaaaggcttt
    12601 ttgagattag tgagtcaata cggctctact tcactttttg ccacagtact tgattttggt
    12661 gtttttgaaa tcgctttgaa atattttgca gcatctgctg ttatagctac cttgtttggg
    12721 cgtgcagtag gcagtatcac atccttcctc attcacaggc attgggtatt taaccatgtc
    12781 accggactgg atattaaagt cttgctcacc aaatacatca tcggtattat cattggtacc
    12841 attcttaatc ttggcggcgt ttggttgcta aatgaagtag ttggcttcgg tccgtggcct
    12901 tcaaggatta ttacatctac tacggtttgg tttattgtgt ttacttataa tcgtctttgg
    12961 gtcttcagca aggggcgcaa tattactacc caattggcaa attcatcagt agtacatgag
    13021 gaggaacata attgaatgtg tatataagtt ttcgaattta taagctatga aacatccgct
    13081 tagtgtgccg tattatagta tttttatgga aaattattgg gcgtgatgtt aggaagaatt
    13141 cgttacatat tcatttttct cttattattc ctttcgtggg ttgtcgtttc gcagaatact
    13201 tcaagtgtgc agggcacagt gaccgatact gtcaccggcg aaggcattcc ttatgcaagt
    13261 attcggattt ccggtcaggt cgaaggcatt acgtctgata tcaacggtaa attttacatc
    13321 aaattggctc aactgccaag taccataact gtttcatata taggttacga aactaaggtt
    13381 gtacagatat tacaaaatac cattcagcca ctgcagatca agctccaacc ttcagcagtt
    13441 gaaatttcgg aagtggaagt cagagttcaa aagtataaga ataaagggaa tccggctgtg
    13501 gagttaatta aaaaggtact tggaaacaaa gaaaaaaacc gcccggaagc attcgattat
    13561 ttccagtata aaaaatacga acaggtagag ttggcattta ataatattgg cgacaataca
    13621 cgtaactcgc ttctgttcag gaaaatgaag ttcatatttg attatgccga caccaaccgc
    13681 acaaatggga aagtattctt accatttttc ttcagagaat cattgtctga tgtatattac
    13741 cgccgctcgc caaatgccga aattgaatac atccgcagtg agtacaacac tgtcgtgcca
    13801 gagttggtgg acaatctggg tgttgccaat tttctgtcaa acctatcaca aagtgtaaat
    13861 atctacgaca actctatcaa tcttgtaacg gttgattttg tgtcgccctt gtcacccatt
    13921 tcgccaaata tttaccgttt ttatatactc gataccggag tggtcaatga taccaaactt
    13981 gtacatctat attttgcgcc tcgcaacaag tcagacaagg catttattgg caatatgtgg
    14041 atagcacttg attcgacata ttctgtacgg aaaatcgacc tgggaattcc gaaggaaata
    14101 aacctcaact gggtgaatga aatgcaaata tcgcaagagt ttgactgggt atctacagtt
    14161 attaccatca atggcaagat gattacatca agaggactga tgataaagtc caacgagtat
    14221 tttatggatt ttgcagtact agacaataaa aaggccaagt cattgttggg tgccaaacgt
    14281 ataaagtatc aagacctaca aataaataga cctatacctg atagtatttt taaagaaaat
    14341 ataaaaatct tccgaaccgt ttcaacggat acaatgtcgc aggcatatct acaacaaaac
    14401 cggccggata cacttacccg cagccagcaa aacatcattg caatggtgga cagcctgcgc
    14461 aatcacaaac ctttcaagaa tctgataaag cttggtaaag tattcatgca tggttatgcc
    14521 gatgtggggc ccgtaagttt tggtcctgtt cataatctat atagctttaa tagtgtagaa
    14581 ggcacaagac tgagagtagg actgcgcacc aatgaaaaat tcagtaagcg tttattgctg
    14641 gaaggctatg cagcttatgg tttgaaggat gaacactgga aaggaaaggc aagtgcaagg
    14701 tattcgctta caaatgctga tgttctgaaa tttccgtata atcaactgcg ggtatggtat
    14761 gaaaatgaag taaaagtgcc cgggcaggaa ctgaatttta tccagccgga taattttctg
    14821 ctttcattca aaagaggtct aacaacaaaa tggtgtacca ggaagtagtt gggctcgaat
    14881 ataccaaaga atcgcagtcg ggtttctctt atgcacttgg agtacggaca agccgacaga
    14941 caccagccgg gcaattgaag tttgaatacc tgcaggacgg ccagctgctt accaaacaca
    15001 gtatgcaact tacacaagtg gggcttacat tgcgttatgc acctaatgag gagttttacc
    15061 agaccttgta cagtcgcatt tctttgtcca ataaatatcc aaagattaca ctcagctatc
    15121 tgcttgcagc aaaagacatt ctgggtggtg aatatagcta tcatagtgtt aaccttcggt
    15181 ttcaaaagct tttttatatt tcaccgcttg gttacagtaa agtgcatatt gatgcaggag
    15241 ctgtatttgg caaagtacca ttcctgttgc tcaaaatcca caacgctaac caaagctact
    15301 cctatcagtt tgacgcttat aacttgatga attatcttga gtttgtgagt gacagatatg
    15361 catcagtgca tatttttcac aactttcagg gaatattgtt caacagactc ccattgatca
    15421 aaaaacttaa acttcgtgag gtagcatctt tcaaggctgt tttcggaggc ttgagtaaaa
    15481 ataataatcc cttcttaaac aacaatgcgg gtctattcgt cttcccaatg gacgcacaag
    15541 gcaggcagac cacctacacg cttgataaag aaccgtatat ggaagcaagt tttggcattt
    15601 ctaacctttt tcggtttttc agactcgatt atgtcatgcg gctcaactat cttagccatc
    15661 ctgatattag caaatggggc attcgattca attttgtatt tcaattctga agaatgctca
    15721 aaacaacttt tgttacacat taccggttat taaatcagta gctttgtggt tcaaaaatga
    15781 tattttattt tattacataa agaattaaac aaaatggcta aaaaagaaag acacaccatc
    15841 cagcttacca aagcagagtt tttggagaaa gtgatggatt acgaaaaaaa ccagaccgca
    15901 tgggtttata aaggagataa accttgtgta atagactttt atgcagactg gtgtggccct
    15961 tgcaaaatgg ttgctcctgt acttgaagaa cttgcggagg aatacaaaga ccacatttat
    16021 atttacaaag tagatacaga agcagagatg gagttatcgg cagtatttgg tatcaggagc
    16081 ataccgacca tcattttttg cccgatgggc gagcaaccac aggcagtaaa tggcgctttg
    16141 cccaaacata atctagaaca aatcatcaaa gaattgttgc taaaggagaa gttgtaatta
    16201 ttgatttaat tttggtgatt ttatagtgat tccggtaaga taaagttcat ctttgccgga
    16261 atcatttttt tattcatata ggcttattcc attacgctat atcagaacca attaagttgc
    16321 ttgtctgatt aatatggaca agtagtgaga aggccggata agcactaagt ttgtgcggga
    16381 gtgagttgga tattgtacaa atcataattt tttctgatgt aatgcttgac aatacgaatc
    16441 caatatcata tatttgtaga atatcttact tactaaccaa aaacttaaac cattatgaaa
    16501 gcacaacaaa atattcgtaa ttatttcgtc ttaaaacaaa ataaaattaa atttaaacgg
    16561 ggggggtaaa attttattct ccagtcttga taatttcatt gttgatttta tttaacaatg
    16621 aattaatgtc tcaatgtccg cctgaaaaaa cttgttttaa taatctgatt aaaaatgaag
    16681 gtttcgagat agattgtgta ccgggtgccg gaatttat
//
LOCUS       DAOQQY010000028        16684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1076_length_16684_cov_5.781947, whole genome shotgun sequence.
ACCESSION   DAOQQY010000028 DAOQQY010000000
VERSION     DAOQQY010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16684
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1076_length_16684_cov_5.781947"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..2366
                     /locus_tag="PLE23_02915"
     CDS             366..2366
                     /locus_tag="PLE23_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HMX82247.1"
                     /translation="MSTTQASVIKFDRNGFSEEKLLQLYRNILLPRMIEEKMLKLLRQ
                     GKVSKWFSGIGQEAIAVGVTTAMDTDELIFPMHRNLGVFTTRNMPLDQLFAQWQGKMS
                     GFSKGRERSFHFGSLEYGIVGMISHLGPQLSLAAGVALAHKLNGEQKISLAFTGEGGT
                     SEGEFHEALNTAAVWKLPVIFLIENNGYGLSTPTSEQYVCENLADRGIGYGMKSLIID
                     GNNILEVYKTIAELSELMKKDPQPVLLECKTFRMRGHEEASGTKYVPQELMDHWAVKD
                     PVSNYEQFLLDEEIISEEEILSIRKEYKELIEENLAIADAEPPVASSYEKELVDVYAA
                     FDFHEISPDHTKGRELRFIEAITEGLNLAMQKFPQLILMGQDIAEYGGAFKVTEGFVK
                     QYGNDRVRNTTLCESAIIGIALGLSLKQRKSMVEMQFADFVTCGFNQIVNNLAKIHYR
                     WGANADVVVRMPTGAGVGAGPFHSQSNEAWFAHTPGLKVVYPSNPADAKGLLLSSFED
                     PNPVIFFEHKGLYRSLSGMVPEGYYTTPIGKAASARQGTQATIITYGMGVHWALHTAE
                     EMGVDVEIIDLRTLLPIDFDTIADSVIKTNRVIILHEDTMFGGIGGEISAWINENLFK
                     HLDAPVMRCASLDTPVPFAIELEKMFLPTERFKEKLQELLNY"
     gene            2450..2863
                     /locus_tag="PLE23_02920"
     CDS             2450..2863
                     /locus_tag="PLE23_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82248.1"
                     /translation="MQYEFDKLIALTGQQSLFHMIANRGNGLILEDIGTKRRQFYSAR
                     QNQFTPMASIGIYTDDDSIELKEVFIRMRNSEEQLPENLNEASALVSYFEKIMPEYDR
                     DRVKINDMKKVIKWFSFLKNHELLSVLDKKEETEK"
     gene            2875..3624
                     /locus_tag="PLE23_02925"
     CDS             2875..3624
                     /locus_tag="PLE23_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX82249.1"
                     /translation="MYKGKKVIVVLPAYNAALTLEKTYQEIPRDLVDEVILCDDASKD
                     NTVDVARNIGIPHILMHESNKGYGGNQKSLYRKALELKGDIVIMLHPDYQYTPKLIPS
                     MVNIIGEELYPVVLGSRILGKGALAGGMPMYKYIANRLLTMTQNFLVGYKLSEYHTGF
                     RSFNRQVLETIPFEENSDDFVFDNEMLSQVIYAGFHIGEVTCPTKYFKEASSINLQRS
                     IKYGLGVLRVSFGHLFQKWGLARLKRYQLNR"
     gene            3780..4580
                     /locus_tag="PLE23_02930"
     CDS             3780..4580
                     /locus_tag="PLE23_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HMX82250.1"
                     /translation="MDEFLLNAGKRDELKNFLDFYVEKFNQPNFIDNDPISVPHSFTL
                     PQDIEIMAFWTSMLAWGNRKSIIRSALRLAELMDGAPYEFIVHHKELDRTRFIKFAHR
                     TFNDTDAIYFIEFFQQYYLQHPSLEDAFARFLTPQDTDTAKAIAGFHKLFFDNAYAPN
                     RTRKHIATPERKSTCKRMNMFLRWMVRNDGKGVDFGLWKKISPSQLLMPLDVHVDKVA
                     RHLGLIERKQTDWNTVLELSETMKVLDPVDPVKYDFALFGLGVEKYFT"
     gene            4756..5106
                     /locus_tag="PLE23_02935"
     CDS             4756..5106
                     /locus_tag="PLE23_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMX82251.1"
                     /translation="MNQMKNNVQLMGFVGRDPEILSFNGDKQMVKLSLATSDRYLSTT
                     GEYKSDTQWHTAVAWGKTAEFIHQNIKKGNEMAINGRLQYRTFENKNGNKQTVAEIIV
                     SEFVKITKEKELPF"
     gene            5302..6243
                     /locus_tag="PLE23_02940"
     CDS             5302..6243
                     /locus_tag="PLE23_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMX82252.1"
                     /translation="MKNTCKTTFLSLILFLLTSQIHAQTEPIAESKFQKEIKPEVSKK
                     LAELEKMMNSGQIKDEEELTSKMYDIIYDIESKSNPVPNPGWDSIAVEDQQVASMEME
                     DTTDYSIINKMPKKKKSFSMNTVMKLGFATAFEGKSNPADVPDINFWRSAMLEVGLNF
                     RNALNSNGTVGVNYGINYLFYDFDTKNQHLNYAGSKDKIAFTKDDDLKDSDFGASYLS
                     IPVGIDIKISKLRVDVGGYGAILLNGFVSLEKKTALGETVFVSSYAKYNMNSFIYGAR
                     LGLGFKNIGLYANYNLNSMFKDTYNLNPFSVGIKFGF"
     gene            6921..8963
                     /locus_tag="PLE23_02945"
     CDS             6921..8963
                     /locus_tag="PLE23_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82253.1"
                     /translation="MKKFYPMFLCVAVALMLPFMVNGQAKKLIMFEHFTQASCGPCAQ
                     QNPVFHPVYLANKHNVNHVAYHTSWPGVDPMNAANPTEVQTMVDLYKVSGVPNMISNG
                     VNIGSPTAATQELINQAGSSPVRISVEDIDLGGGNHQVKVKVYTLADVPPATYIMRTL
                     LAEENVTYTSAPGSNGEKEFPNVFRKFINTNGSAGDAYVPAQVGEFTEVSYNYTVDPS
                     WKENEIYPIAYIQNVSTKEVIQSGSSRDEKVEAVNVETKVFQVGNLVDGNEFKFNLTN
                     LTENDAQLQVSIQGTWPADWGAYVDINGAHYNSGDTLIIPANATINATLKATVGPTPG
                     IGDFSAGVFNTETNTLQAFAFTVISNITDLLIVTDNKPAEGNPDLKKNYNDGLVATGE
                     PAYASIGATKAVKGFEAGAMGTVRHIYYSVGWTFPAMTPDMVGYFQDFLDNGGNIMVA
                     GQDVNWDMASGDASANGNAASKALMNNYFNSNFIDDGSSANLQFKPVAGDVIFDGVGS
                     SGLNKSYGATYFYPDQIEAVGPEGYNIFTYNTAAKVAGVRSKHNNFKTVNLGIGLEMI
                     AVKAVADEIMKRTYDWFHGKITDTEFDNYLNAAMGQNYPNPADKFTVINFGTETTDDF
                     ILNIVDLTGKMVMSKNIAKGTSSESINTEALPQGIYQYYLTDGSKVTTAKKLVVIH"
     gene            complement(9071..11443)
                     /locus_tag="PLE23_02950"
     CDS             complement(9071..11443)
                     /locus_tag="PLE23_02950"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMX82254.1"
                     /translation="MKKGFFFLILCLLLQYIPYGYAQNIQNNPKSNHGNKFEQLGTIL
                     PDANSYRTASGAPGYRYWQMRADYDIEARIDESTLQLFGSEWITYYNNSPEPLQYLWL
                     QLDENEHDPNSDSNTFNESQIKDPYTAPSIEGLDRRGKLQGYGCKIDEVKGAAGQNLP
                     YTINQTMMRVDLPISLMPGKSIKFKVKWHYKIPNRMTIGGRGGLEYFPEDGNHLFTIT
                     QWYPRMCVYSDFQGWQNRQFTGRGEFALTFGNFNVKMTVPADHIIAATGECQNYKEVL
                     SPERYQRWMQAQNSKEIVEIVTLDEAKKAEQSKSSKTKTWIYKAENVRDFAWGSSRKF
                     IWDAVPEMIEGKKVMCMSYYPKEAYSLYRKYSTKVVAHTIKTYSKYTIAYPYPVAISV
                     EASNGMEYPMICFNFGRTEKDGTYSEGTKYGAIGVIIHEVGHNFFPMIINSDERQWSW
                     MDEGLNTFVQFLTEQEFDNNYPSQRGPAHKIVDYMSLPKDQLEPIMTNSDNIIQFGPN
                     AYAKPATGLNILRETIMGRELFDYAFKQYCQRWAFKHPTPADLFRTMEDASGVDLDWF
                     WRGWFYDIDPVDISLDSVRAWTFSNSPDKEFALESPQISKEKNFESISKIRNREAKLN
                     FAVDRDTSLRDFYYYNPDIENGKKTNSNMEVMPDSNFNKYKDTWVYELHLSNKGGMVM
                     PVIIQWNYEDGTNETERISAHIWRKNEHNIVKTFAKPKKVATILLDPMRETADINEDN
                     NAWNAPPHPTKFQLFQYKQQPQFGRRGTRNANNPMQQAKQKQQEGNNSSN"
     gene            complement(11448..12020)
                     /locus_tag="PLE23_02955"
     CDS             complement(11448..12020)
                     /locus_tag="PLE23_02955"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HMX82255.1"
                     /translation="MTDFQLWFTTGLEHILDPGGLDHVAFIIALTIMFPLRDWKQLLL
                     QITAFTLGHSLTLALSVLQIVNIKQSIIEILIPLTIIFTAAHNLYSIKYNKPFRKSNY
                     IFALFFGLIHGLGFSYLLRSMLSKEDSIVSPLFAFNLGLEAGQIIVVGILSILSLILS
                     FYWRIPEKKIQTFGSLLVLVFSLYLLTNRF"
     gene            12041..12592
                     /locus_tag="PLE23_02960"
     CDS             12041..12592
                     /locus_tag="PLE23_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82256.1"
                     /translation="MIKLTKYASKLWNFLPILKNIGRIVSLICLSGLFVGFHPFYVSV
                     TEINYDKTERVVQVSCKIFTDDFENTLRKQTNVTVDLINPKDRSKMESLIKDYIEKNL
                     IISADGKQLKYSFLGYEKEEEAIWSYFITEPIAQPKRLSVINTLLYDFKKEQINIVQV
                     TGLGNKQSSKTTWPEKKLEFEYK"
     gene            complement(12654..13604)
                     /locus_tag="PLE23_02965"
     CDS             complement(12654..13604)
                     /locus_tag="PLE23_02965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX82257.1"
                     /translation="MDQQLQISMVIPSFNEEESLPELTAWIAQVMAREGYSYEVLLID
                     DGSTDQSWNVIKKLASADHHIRGIRFRRNYGKSAALNTGFQASRGKVVITMDADLQDS
                     PEEIPELYKMITTDGYDLVSGWKQKRYDNTFTKNIPSKLYNWANRKISGIKLHDMNCG
                     LKAYNSKVVKSIEVYGEMHRYIPVIAKGAGFKKIGEKVVQHRARKYGSTKFGMERFIN
                     GFLDLLTIAFMNKFSKRPMHLFGTLGVLSFIIGSFILIYLSIMKLMYAQYGIASRPLF
                     FFGILALIIGSQLFVTGFLAELMVRNSAVRNLYQIEEEIG"
     gene            complement(13648..14157)
                     /locus_tag="PLE23_02970"
     CDS             complement(13648..14157)
                     /locus_tag="PLE23_02970"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HMX82258.1"
                     /translation="MENSGVKWGVIGAVATVLISLLTYIVGQKFYLQAGSWIGMFAGL
                     FFLYKSGIEARQQAGGYISFQEVLKSVFTTYIIMTVASTIMQYVMYKFVDPGLIDVTK
                     QIAMEGMEKMKGILGEEGYEEAMEQMETQDFAPSIRQSLLGLAFSIILGFGVSAIFAA
                     IIKRKQPEY"
     gene            complement(14182..15450)
                     /locus_tag="PLE23_02975"
     CDS             complement(14182..15450)
                     /locus_tag="PLE23_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMX82259.1"
                     /translation="MRILIKNTKIVHHNTGLSDSEQDILIDNGIIREVNNNISQKADK
                     IIEAKGSYTSSGWTDVGTRTGEPGYEHREDIDSVTEAAAAGGYTTIITTPNNIPPTQS
                     KSDILYLKQKSEGKLTNILPMAALSFDCAGKDIAELYDLHSSGAIAFTDGPRSIQDTG
                     LLKRGLEYVQAFGGLILHSPVNGEFNKGWQMNEGIISTSLGMKGLPDIAEVMMVERDL
                     RILEYTGGRLHLWNISTQGSVELVRKAKKQGLNISCSVPVMNLCFDDGALTGFDTNFK
                     LSPPLRTAEHRNALWEGLVDGTIDFVTSEHTPLENDVKDLEFPYAKFGTIQLETAYAI
                     LQTHYGKKLTPQLTEKIFSQGANTIFGLNKPDIKPGEKADLTVFDPNAEWVYLQTKIK
                     SKSNNNPLIGKKLKGKAIATIKGNLIFAEN"
     gene            complement(15465..>16684)
                     /locus_tag="PLE23_02980"
     CDS             complement(15465..>16684)
                     /locus_tag="PLE23_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82260.1"
                     /translation="GNLNIAANATVTDTVHVAINKTGWHQAELVITDYPIQFDDHYYF
                     TFNVAEQIDVLCINEDLPNRYLEAAFKGISYFRLNSVNNKTIDYSKLGNNKLIILNEL
                     KSISSGLAQELVNYMKNGGNVLIFPAENSDLASYNAYFNSIQAHQLQAFSKTERKVAS
                     INTNDFVFSDVYENAKANLKLPATTGSFPIQEFAGKAVVKLLTNRDGSTYLAKYKVEK
                     GNQYLCFAPLDQQYNDLVNSAEVFIPMLYKMSISTTKASKIAYTLDKDAYIESDMNTA
                     DKSDKVFRLENDKTSIIPEQKTIQSHLVMHVNNLIKEAGFYTLVDDNKKKLQDFAFNF
                     NRKESDLLCLTKDEIKAAVSGNVKLLDINQDTNLSTMVGEKELGIILWKWFVVLALIF
                     LALESLIIRFWKV"
BASE COUNT         4917 a         3157 c         3479 g         5131 t
ORIGIN      
        1 gcctgttcca acgcaggaag aatttcaaaa tattttttcc tccccttcta aaaaattaaa
       61 acacccgaca cacaaaccaa ctcgacaaag gcacggaaac tcaatgatga caatggtttg
      121 gaaggatgtt cgagaagagt accattggct ctaacaagcg gtttgaacgc tcggcgggta
      181 aagtactttg attgaacttt ttcggtaaga aaaatgtatt attttcatat caacatttat
      241 cggtaaaaat ctgccttgtg tcaagccgcc aaaacggtta aaataacacg ttaaatgcaa
      301 aggaataacg gtaatattcc ccaaaatcat agtatctttg cgcctcattt taaataacca
      361 tatacatgtc cactacccag gcatcggtaa taaaatttga ccgaaacgga ttttcggaag
      421 aaaagctgtt gcaactctac agaaatattc tgttgccacg tatgattgaa gaaaaaatgc
      481 tcaaactgct tcgtcaaggt aaggtgagca aatggttttc aggcataggg caggaggcaa
      541 tagcagtcgg tgtcactacg gccatggata ccgatgaact gatattccca atgcaccgca
      601 atctaggagt atttactaca cgcaatatgc cacttgacca gttgttcgcg cagtggcaag
      661 gaaaaatgtc aggtttttca aagggtagag agcggtcttt ccattttggc tcactcgagt
      721 atggtattgt aggaatgata tcgcaccttg gcccgcagtt gtcacttgca gctggagtag
      781 cacttgccca taaactgaat ggcgagcaga aaatatcgct tgcatttact ggtgaaggcg
      841 gtacgagtga aggcgaattc catgaagccc ttaatactgc tgctgtgtgg aaactaccgg
      901 ttattttcct gatcgaaaac aacggctatg gtctgtcaac gcctacaagc gagcaatatg
      961 tatgcgagaa tctggcagat cgtggtatag gctacggcat gaaatcactc atcattgacg
     1021 gcaataacat cttggaagtt tataaaacca tcgctgaact ttccgagttg atgaaaaaag
     1081 atcctcaacc ggtgttgctt gagtgcaaga cattccgcat gcgaggacat gaagaagcct
     1141 ctggtaccaa atacgtacca caggaactga tggatcattg ggctgtgaag gatcctgtta
     1201 gcaattacga acagtttttg cttgatgaag aaattatctc agaagaagaa atattgagca
     1261 ttcgcaaaga atacaaggag ctgatagaag aaaatctggc cattgctgat gcagaaccac
     1321 cggtggcctc tagctacgaa aaagaacttg tcgatgtata tgcagctttt gattttcatg
     1381 aaataagccc cgaccataca aagggacgcg aactgaggtt tattgaagct attaccgaag
     1441 gtcttaacct tgccatgcaa aaatttcctc aactcatact catgggccaa gatattgcag
     1501 aatatggcgg tgcttttaaa gtaactgaag gattcgtaaa acaatacggt aatgatagag
     1561 tgcgcaatac aacgctttgc gaaagtgcca tcattggtat agctttgggg cttagcctta
     1621 aacagaggaa atcaatggta gaaatgcagt ttgcagattt tgtaacctgt ggcttcaacc
     1681 agatagttaa taatcttgcc aaaatccatt atcgttgggg tgcaaatgca gatgttgtag
     1741 tgcgtatgcc taccggtgca ggcgtgggtg ccgggccttt ccattcgcag agcaatgagg
     1801 catggtttgc tcatactccg ggtcttaagg tggtttaccc gtcgaacccg gctgatgcca
     1861 aaggactgct cctttcatca tttgaagatc cgaatccggt tattttcttt gaacataaag
     1921 gcctgtatcg cagtcttagt ggcatggtgc ccgaaggtta ttatactaca ccaataggca
     1981 aggctgcatc tgcccgacaa ggaacacagg ctacgattat aacttacggg atgggcgtgc
     2041 actgggcatt gcatacagca gaagagatgg gcgtagatgt cgaaatcatc gatcttcgta
     2101 ccttgctgcc gattgatttt gatactattg ccgacagtgt cattaaaaca aaccgagtca
     2161 ttatccttca tgaggatacg atgtttggtg gtattggtgg tgagatttca gcttggatta
     2221 atgagaactt attcaagcat ctcgatgcac cggttatgcg ctgtgcttca cttgatacac
     2281 ctgttccatt tgccattgag ctggaaaaaa tgttcctacc gacagaaaga tttaaagaaa
     2341 aattgcaaga attgctcaat tattaaacca ggaggaaaaa atttaaattt taataaaaaa
     2401 caatgcgaaa cccaaaattg gaataatttc gcaagcaaaa acacaaaata tgcaatacga
     2461 atttgataaa ctcatagcat tgaccggtca gcaaagtctt tttcacatga tcgccaatcg
     2521 tggtaatggc ctgattcttg aagatatcgg tactaagaga aggcagtttt attccgcaag
     2581 gcaaaatcag tttacaccga tggcatctat cggtatttat acagatgacg actctatcga
     2641 gctcaaggaa gtgtttataa gaatgcgaaa ttcggaagaa cagttgcccg aaaatttgaa
     2701 tgaagcatct gccttggtgt cttattttga gaaaattatg cctgagtatg accgcgacag
     2761 ggtaaaaatc aatgacatga agaaagtgat taaatggttt agttttctta aaaaccatga
     2821 attgctctca gtactagaca aaaaggaaga aaccgaaaaa taaaacaata acgaatgtac
     2881 aaagggaaaa aggtaatagt tgtattgcct gcctacaatg cagcacttac actcgaaaaa
     2941 acttaccagg aaataccccg cgatctggtg gatgaggtca tcttgtgcga tgacgcaagt
     3001 aaggataata cagttgatgt tgcaaggaat attggtattc cacatatact gatgcacgaa
     3061 tcgaacaaag gctatggcgg taaccagaaa agcctctaca gaaaagcact cgaactgaaa
     3121 ggcgatatcg tcatcatgtt gcatcccgat tatcagtaca cacccaagct cattccgtca
     3181 atggtcaaca tcatcgggga ggaactttat ccggtagtac ttggttcgag aatactcggg
     3241 aaaggggcac tggcaggtgg tatgcccatg tacaaatata ttgccaaccg acttttgaca
     3301 atgacgcaga attttctcgt cggttacaag ttgtcagaat atcatactgg tttccgttca
     3361 tttaatcgtc aagtactcga gactattcct tttgaagaaa attctgatga ttttgttttt
     3421 gataatgaaa tgctcagcca ggtgatatat gcgggtttcc atattggaga ggttacctgt
     3481 cctaccaaat attttaagga ggcaagttct atcaatttgc agaggagcat taagtacggt
     3541 ttgggtgtac tccgggtgtc atttgggcac ttgtttcaga aatggggttt ggccagatta
     3601 aaaagatacc aacttaaccg ctaaggtttt tcttgaaggt ttatggcttt cttaatgaaa
     3661 aatattgtag ccaaacatag cgtataaatc agtgtagctg tcataagttt acattatagt
     3721 tgctaaatta acagttattc ttcatatcaa agtttttttt caaatatttt ttaccattta
     3781 tggatgaatt tctgttaaat gccggaaaaa gagatgaatt gaagaatttt ttggactttt
     3841 atgttgaaaa atttaaccaa cctaacttca tagataacga cccgatttct gttccgcatt
     3901 cctttacatt gcctcaggat attgaaataa tggcattttg gacatctatg cttgcatggg
     3961 gtaacagaaa gtccatcatc cgaagtgcac tcaggcttgc agagttaatg gatggtgcac
     4021 cttatgaatt cattgttcat cataaggaat tggacagaac aagatttata aaatttgccc
     4081 accgcacgtt caatgataca gatgctatat attttattga gttctttcag caatattacc
     4141 ttcagcatcc gtcacttgaa gatgcatttg caaggttttt gacacctcaa gatacagata
     4201 cagccaaggc aatagcagga ttccacaagc tgttttttga taacgcttac gctcctaata
     4261 ggacacgtaa gcacattgct acgccggaaa ggaaaagtac atgcaagcga atgaatatgt
     4321 tcttacgatg gatggttaga aatgatggga aaggtgttga ttttggttta tggaaaaaaa
     4381 tatcaccctc ccaattgctc atgccgcttg atgttcatgt cgataaggtg gcacggcatc
     4441 ttggactgat agagcgaaag caaaccgatt ggaatactgt gcttgagttg tcggaaacaa
     4501 tgaaagtact tgatcctgtt gatccggtta aatatgattt tgcgcttttt ggtctaggcg
     4561 ttgaaaaata ttttacctaa tttttactag attaaagtga tgaaatacaa tataatacat
     4621 attattaaat taaaattatt caattttttt aaaatatttt atggaaaaat atgaaacgct
     4681 tcgtctatgt agtgtctttt cgatcgaagg cattgagtgt taaaagcaat tatttaaagt
     4741 taaaaagcaa gtatcatgaa tcagatgaag aacaacgtac aattaatggg ttttgtaggt
     4801 agagatccgg aaatcctaag cttcaatggc gacaaacaaa tggtaaaatt atctttggcg
     4861 acgtcagaca ggtatctttc tacaacaggt gaatataaaa gtgacacgca gtggcataca
     4921 gcagtagcct ggggcaaaac cgcagaattc atacatcaga acatcaaaaa aggtaacgag
     4981 atggccatta atggaagatt gcagtaccgt acttttgaaa acaaaaacgg caataagcaa
     5041 acagtggcag aaataattgt tagtgaattt gttaagataa ccaaagagaa ggaacttccc
     5101 ttctaatcta taagtgtgtg tttgccccag ttcggaatgg attggggctt tttgattggt
     5161 ggtaatatgc catagtaaat tccttagatt tgttacaaga tttaattttc taagaaaggc
     5221 aaataattga ttaattgtcc ctaactttga ttctggctat catttaatta accatttaat
     5281 tataatactt aaccaataat catgaaaaat acctgtaaaa ccacatttct gagtctaata
     5341 ctattccttc ttacctcaca gattcatgct caaacagaac caattgcaga atcaaaattc
     5401 cagaaggaaa taaagcctga ggtcagtaaa aagttggcag agttggaaaa aatgatgaat
     5461 agtgggcaga tcaaagatga ggaagagctc acttcaaaaa tgtacgatat aatctacgat
     5521 atagaatcta aatcaaatcc tgtacctaat ccgggctggg attctattgc tgtggaagat
     5581 caacaagtag cttcaatgga aatggaagat acgacggatt attcaatcat caataaaatg
     5641 ccaaagaaga agaaatcatt ttccatgaat accgtcatga agcttggttt cgcaactgct
     5701 tttgaaggga aatccaatcc tgctgatgtg cctgacataa atttttggag gtctgctatg
     5761 cttgaggtag ggcttaattt caggaatgca ctgaattcaa atggtacggt cggtgttaat
     5821 tatggtatca attatctgtt ttacgatttt gatacaaaaa atcaacatct caattacgca
     5881 ggaagcaaag ataaaattgc attcactaaa gatgatgact taaaagattc agatttcgga
     5941 gcaagctatt tgagcatacc tgtgggtatt gacattaaaa tttcaaaact gagggttgat
     6001 gtaggaggtt atggtgccat cctgctcaac ggatttgtaa gtcttgagaa aaaaacagcg
     6061 ttgggagaga cggtttttgt ctcttcttat gccaaataca atatgaatag tttcatttat
     6121 ggtgcacgtc tcggacttgg attcaaaaat atcggcctgt atgccaatta caacctgaac
     6181 tcgatgttta aagatacata caatctcaat ccgttttcag taggcataaa atttgggttc
     6241 taatatacgt aaataagagt gaaaggacct gatacagcaa tagctattga gtatcttgac
     6301 tcgcctgtag gggtttctta aaatcgttgt ttgcggagaa tcgctccgtc aacttgattt
     6361 tgtatcatcg cgtactgagc cggagacgcc aaccgcatat acagaacgag ttaagctaca
     6421 actagaagag tattttcgtg ggacgagaca gagtttccaa ctatctcttg cacctgaagg
     6481 tacgcctttt cagaaagcag tatgggagaa attaattgag attccttatg ctaatttgat
     6541 aagttacaaa gatctggcat tgaagttagg agatatgaaa aagattcggg cagtaggatc
     6601 cgcaaatgga agaaatccaa tcacgattat catcccatgt caccgggtta ttgggaacga
     6661 taaaaacctt atcggatatt caggaggtct tcacataaag cggtggctga ttgaccacga
     6721 aaatgcagga atgcaaaaca ccttgtttgg ttaagatttg agagggggag agtgatcttg
     6781 tggtgtgggc ttaaataatt ggtactgcaa cggctattat gtctaaatta tatataaaat
     6841 gggcattttt tgaatattta tgtatgccgg atagtatatt tgtacttata aataattttt
     6901 cttaattaaa aaaatagcta atgaaaaagt tttacccaat gtttttatgt gtggctgttg
     6961 ctttaatgtt gcctttcatg gtcaacggtc aagcaaagaa gctcataatg tttgaacact
     7021 tcacacaagc atcatgtggc ccgtgtgcac agcaaaatcc ggtttttcac ccggtttatc
     7081 tagccaacaa acataatgtt aaccatgtag cttaccatac ttcatggccg ggcgttgacc
     7141 ctatgaatgc agccaaccca acagaagtgc agacaatggt tgacttgtat aaggtatccg
     7201 gtgtaccgaa tatgattagt aatggtgtca acattggcag tcctactgct gctacacaag
     7261 aactcatcaa tcaggcagga agcagtccgg taagaatttc agttgaagat attgatctgg
     7321 gtggcggaaa tcaccaggtg aaggtcaaag tatatacact tgctgatgtg ccaccagcta
     7381 catatattat gcgtacactt cttgccgaag agaatgtcac ttatacaagt gcaccgggta
     7441 gcaacggcga aaaagaattt ccaaacgtgt tccgtaaatt tattaatact aatggcagtg
     7501 caggtgatgc atacgtaccg gctcaggttg gagaatttac tgaagtaagc tacaactata
     7561 ctgttgaccc aagctggaag gaaaatgaaa tctacccgat agcatacatt cagaatgtta
     7621 gcaccaaaga ggtaatccaa tcaggttctt caagggatga aaaggtagaa gctgtgaatg
     7681 tcgaaaccaa agttttccag gtaggaaacc ttgttgacgg aaatgaattt aaatttaacc
     7741 tgacaaacct tactgaaaat gatgcacagc ttcaggtctc tattcaggga acctggcctg
     7801 cagactgggg tgcgtatgtt gacatcaatg gtgcacatta caattctgga gatacgctga
     7861 tcattccggc aaatgcaact attaatgcaa cactcaaagc aaccgttggt ccaactccgg
     7921 gcattggtga ctttagtgca ggtgttttta atacggaaac caacacacta caggcctttg
     7981 catttaccgt catctctaac attaccgatt tgttaatcgt aacggataat aaaccggctg
     8041 aaggtaaccc cgatctaaaa aagaattata atgacgggct agtagctaca ggtgagccgg
     8101 catacgcatc aatcggtgca acaaaagcag tgaaaggttt tgaagcaggt gctatgggca
     8161 cagtacgtca tatttattat tctgtaggat ggactttccc agctatgact cctgatatgg
     8221 tagggtattt ccaggatttt cttgataatg gtggaaacat tatggttgca ggacaggatg
     8281 taaactggga catggcttcg ggtgatgcca gtgcaaacgg aaacgcagcc tcaaaagcct
     8341 tgatgaataa ttatttcaat tctaatttca ttgacgatgg ttcaagcgct aatcttcagt
     8401 ttaaacctgt ggccggagat gttatttttg atggagtagg ttcatcaggt ttgaacaaat
     8461 cttatggtgc aacttatttc taccctgacc agattgaggc agtggggcct gaaggttata
     8521 atatctttac ttacaataca gctgcaaaag tagctggagt cagatcaaag cacaataact
     8581 tcaaaaccgt taacctcggt atcggtttgg aaatgattgc tgtaaaagca gtagctgacg
     8641 aaatcatgaa gagaacttac gattggttcc atggtaaaat cactgatact gaatttgata
     8701 attatctcaa tgcagcaatg ggacagaatt acccgaaccc ggctgataaa tttacagtaa
     8761 taaattttgg cactgaaacc accgacgatt ttatacttaa tattgttgat ttgacaggga
     8821 aaatggtaat gtctaaaaat atagctaagg gtactagttc agagtcaatc aatactgaag
     8881 cattgccaca aggtatctac cagtattatc ttacagatgg aagcaaagtg acaactgcta
     8941 aaaaattggt agtcattcac tagtttgtat ttttgcaaga atcaaagaca gtcagcctgg
     9001 aaataccgga ctgactgtct tttttttgtt atagaaatca aatgaatgac aatcacaaaa
     9061 gtgattataa tcaattcgaa ctgttgttgc cttcttgttg cttttgtttt gcctgctgca
     9121 tcgggttatt agcatttctt gttcctcttc gcccgaattg tggttgctgc ttatactgaa
     9181 ataattgaaa ctttgttggg tgcggaggag cattccacgc gttattatct tcatttatat
     9241 cagctgtctc tctcatagga tcaagaagaa ttgtagctac ttttttaggt ttggcaaatg
     9301 tttttacaat attatgctca ttttttctcc agatatgggc acttattctt tctgtttcgt
     9361 tggttccgtc ttcgtaattc cactgtataa ttacaggcat taccatgcca cctttgtttg
     9421 aaagatgcag ctcatacacc caagtatctt tgtatttgtt gaaattggaa tccggcatta
     9481 cttccatatt tgaattcgtt ttcttgccgt tttcaatatc cggattgtag taataaaaat
     9541 ctcgtaggct ggtatcgcgg tcaacggcaa agttgagctt agcttccctg ttgcggattt
     9601 ttgatatgct ttcgaaattc ttttctttgc ttatttgtgg agattcgagg gcgaattctt
     9661 tgtccggact attcgagaat gtccatgcac gcaccgaatc cagtgaaatg tcaaccggat
     9721 cgatgtcata gaaccaccct cgccaaaacc agtccaggtc aactcctgat gcatcttcca
     9781 ttgttcggaa caaatcggcc ggggtaggat gtttgaaagc ccatctttgg caatattgct
     9841 taaatgcgta gtcaaacagc tcgcgaccca tgatggtttc tctcaggata ttgagccctg
     9901 tagctggctt tgcataagca tttggcccaa attgtatgat gttgtcactg ttggtcatga
     9961 tgggttcgag ctggtctttt ggtaggctca tgtaatctac aatcttgtgt gccggcccac
    10021 gctgagatgg ataattattg tcaaactcct gttctgttaa aaactggaca aaggtgttca
    10081 ggccttcatc catccagctc cattggcgtt cgtcactgtt gatgatcatt gggaagaaat
    10141 tgtgacctac ttcgtgtatg atgactccta tggcaccata ctttgtgcct tctgagtatg
    10201 taccgtcttt ttctgtgcgt ccaaagttaa aacagatcat cggatattcc attccgttag
    10261 atgcttctac actgatggct accggatatg gataagctat ggtgtatttg gagtaggttt
    10321 tgatggtgtg tgccaccact tttgtagagt attttctgta cagcgaataa gcctcttttg
    10381 gataataact catacacatc actttctttc cttcaatcat ctcaggtaca gcatcccaaa
    10441 tgaatttcct ggatgagccc catgcaaaat cccttacatt ttctgccttg tatatccagg
    10501 ttttagtttt ggatgatttt gactgttctg cttttttcgc ttcgtcaagt gttacaatct
    10561 ctacgatttc tttgctgttc tgggcttgca tccatcgttg atatctttcc ggactgagta
    10621 cttccttata attctggcat tcacctgttg cagcaattat gtggtcagca ggtacggtca
    10681 tttttacatt aaaattgcca aaggtaagag caaattctcc tcttccggtg aattgtcggt
    10741 tttgccaccc ctgaaaatcc gaataaacac acatcctcgg ataccactgt gtgatcgtga
    10801 aaagatggtt gccatcttca ggaaaatact caagtccacc tctgccacct atggtcattc
    10861 tgttggggat tttatagtgc cacttgactt tgaatttgat tgattttccc ggcataaggg
    10921 agatgggtag atcaacccgc atcatagtct gattgattgt gtaaggaaga ttttgtccgg
    10981 ctgcaccttt aacttcatct attttacaac cataaccttg cagtttgccc cttcggtcga
    11041 gtccttctat gctgggcgct gtataaggat cttttatctg gctttcgttg aatgtgttgc
    11101 tgtctgagtt cgggtcgtgt tcgttttcat cgagttgtaa ccaaagatac tgaagtggct
    11161 ccggcgaatt attataatat gttatccatt cagatccaaa taattgtaat gtcgattcgt
    11221 caatccttgc ttctatatca taatcggcac gcatttgcca ataacggtac cctggtgccc
    11281 cggaagccgt gcgataactg ttggcatcgg gtaggatggt gcccaattgc tcaaacttat
    11341 tcccgtggtt agactttgga ttgttctgaa tattttgtgc gtaaccatac gggatatatt
    11401 gcaaaagcaa acagagtatt agaaagaaaa aacctttttt cattttgtta aaatctgttg
    11461 gttaataagt ataacgaaaa cacaagcaca agcagtgagc caaatgtttg tatttttttt
    11521 tccggaattc tccaataaaa agataaaatt aaggataata ttgataatat gccaactact
    11581 atgatttgtc cggcttccag gccaagattg aatgcaaaaa gtggactcac aattgaatct
    11641 tcttttgaaa gcattgaccg tagcagatat gaaaatccaa gcccgtgtat aagaccaaag
    11701 aataatgcaa agatgtaatt cgatttgcgg aacggtttgt tgtatttgat tgaataaagg
    11761 ttatgagcag ctgtaaaaat gattgttaat ggaatcagta tttcgatgat gctttgtttg
    11821 atgtttacta tctgcagtac actcaatgcc agtgtcagcg aatgccccag tgtgaatgct
    11881 gttatttgta gcaacagttg cttccagtcg cgcaatggga acataatggt aagagcaatt
    11941 ataaatgcga catgatccaa tccacctggg tcgagtatgt gttcaaggcc ggttgtaaac
    12001 caaagttgaa agtctgtcat ttgctgctta tctttgtgca atgataaagt taaccaaata
    12061 tgcatcaaaa ctttggaatt ttctaccaat tttgaagaat attggacgaa tagtatcttt
    12121 gatatgtctg agtggtttgt ttgttggctt tcatccgttt tatgttagtg tcacggagat
    12181 taattatgat aaaaccgaac gtgtggtaca agtcagttgc aagattttta ccgatgattt
    12241 tgaaaataca ctccgtaaac aaacaaatgt taccgttgac ctcatcaatc cgaaagaccg
    12301 ttcaaaaatg gaatcgctca ttaaagatta tatcgaaaag aatctcatca tttcagccga
    12361 cggcaaacag ttgaaatatt cgttcctcgg gtatgaaaag gaggaagagg ctatctggag
    12421 ctatttcata acagaaccga tcgcacagcc taaacgactt tctgtaataa atacgctctt
    12481 gtatgatttc aaaaaggaac agataaacat cgtgcaagtt acgggtttgg gtaataaaca
    12541 aagcagcaag actacatggc ctgagaagaa actggaattt gaatataaat aaccggaaat
    12601 gatttttcct gagtaaagaa ttggcacctt tttgttgaaa tatcaaagat gtgctacccg
    12661 atctcttctt cgatttgata aagatttctc actgccgaat tcctgaccat gagttctgcc
    12721 agaaaacctg ttacgaataa ctggcttccg ataataagtg ctaatatacc gaaaaagaat
    12781 aaaggcctgc ttgcaattcc atattgtgca tacattagct tcatgatgct caagtagatg
    12841 agtataaagg aaccaattat aaacgaaaga actcccagtg tgccaaacaa gtgcataggt
    12901 cttttgctaa atttgttcat aaaagctatg gtcagtaagt caagaaaccc attgatgaat
    12961 ctttccatac cgaacttggt gcttccatat tttctggctc tgtgctgtac caccttttca
    13021 ccaatttttt taaacccggc cccttttgca attacaggaa tgtatcggtg catttctcca
    13081 tagacttcta tacttttaac tactttcgag ttgtaggctt taagtccgca gttcatgtca
    13141 tgcagcttta ttccacttat ttttcggttt gcccagttgt ataatttgct tggaatattt
    13201 ttggtgaatg tgttatcgta ccgtttttgc ttccatcctg ataccagatc gtagccatca
    13261 gttgttatca ttttgtacag ttcgggtatt tcttccggac tgtcttgcag gtcagcatcc
    13321 atagttatga ccacttttcc tcttgaagcc tggaaaccag tgttgagcgc tgcagatttt
    13381 ccataattac gcctgaaccg gataccacgt atatgatgat cggctgaagc taattttttg
    13441 atgacattcc aactttggtc ggtactgccg tcgtctatta aaagtacctc gtaactatag
    13501 ccttcacgtg ccattacttg agcgatccat gcagtaagtt ctggcaagga ttcttcttcg
    13561 ttgaatgatg gtatgaccat cgatatttgc agttgctggt ccaaatcgtg tatgtagttt
    13621 gcttcttgta tttctgtcgc gtgcagatta atattcaggt tgttttcttt ttataatagc
    13681 ggcgaaaatg gcactcacac caaaacctag gatgatgctg aatgcaagcc caagcagcga
    13741 ttgtctgata gatggtgcaa agtcctgcgt ttccatttgt tccatggctt cttcataacc
    13801 ttcttcaccg agtatgcctt tcattttttc catgccttcc attgctatct gtttggttac
    13861 atcaattaag cccgggtcaa caaatttata cattacgtat tgcatgatgg tacttgctac
    13921 agtcataatg atgtatgtag taaatacaga ttttaatact tcctgaaaac ttatatatcc
    13981 tccagcttgc tgacgtgcct caataccaga tttgtacagg aaaaataaac ctgcgaacat
    14041 gccaatccaa gagcctgcct gcaaataaaa tttctgcccg acgatgtacg taagcaatga
    14101 aattaataca gtcgcaactg cacctattac accccattta acgccagaat tttccatagc
    14161 tgtcaattta ttattttgtt attagttttc agcaaatata agatttcctt tgatagttgc
    14221 tattgctttt cctttgagtt tttttccgat gagtggattg ttatttgatt ttgacttgat
    14281 ttttgtctgc aaatataccc attcggcatt tgggtcaaat acagttaagt cggctttttc
    14341 tcctggttta atatcaggtt tattgagtcc gaatatagta tttgctcctt gggagaatat
    14401 tttttcagtc agctgtgggg tcaatttttt tccgtaatgt gtctgcaaga tggcataggc
    14461 tgtctcaagt tgtatagtgc cgaatttagc ataggggaac tccaggtctt tgacatcgtt
    14521 ttcaagtggg gtgtgttctg atgtcacaaa atctatggtg ccatccacta agccttccca
    14581 taatgcattg cggtgttcag cagttcgtaa gggtgggctc agcttaaaat tagtatcaaa
    14641 tcctgtaagt gcgccgtcat cgaaacataa attcatgacc ggaaccgaac aactgatatt
    14701 gagtccttgt tttttggctt ttctcaccaa ttctacagag ccttgggttg aaatattcca
    14761 caaatgcagg cggcctccgg tatattcgag tattctcaga tctcgttcta ccatcatcac
    14821 ttcggcaata tccggcaggc ctttcatacc tagtgatgta ctaataatgc cttcattcat
    14881 ttgccaccct ttgttaaatt caccattgac aggtgaatga aggatgagcc caccgaatgc
    14941 ctgtacatat tcaagtcctc gtttgagcaa tccggtatcc tgtatgcttc ttgggccatc
    15001 tgtaaatgca atggcaccgc tggagtgtag gtcgtatagt tctgcaatat ccttccccgc
    15061 acagtcgaat gataatgcgg ccattggcag aatgttcgta agctttccct ccgatttctg
    15121 tttcagatac aagatgtcag acttgctctg tgtgggtggt atattgttgg gagtggtgat
    15181 aatggtcgta taacctccgg ctgcagcagc ttcggtgaca gagtcaatat cttctcgatg
    15241 ttcgtacccg ggttcgcctg tgcgtgtacc cacatcggtc cagcctgatg aagtataact
    15301 acctttggct tcaataatct tatcagcctt ttgtgaaata ttgttgttta cttctctgat
    15361 gataccattg tctataagta tatcttgctc agaatcagag agtccggtgt tgtggtgaac
    15421 gatttttgtg ttttttatta aaattcgcat cgattaattt agcattaaac tttccagaat
    15481 cggataatca gcgactccaa agctaagaaa attaatgcta atactacaaa ccatttccat
    15541 aaaataattc caagttcttt ttcacctacc atggtactca agtttgtatc ttgatttata
    15601 tccaataatt ttacattgcc ggagacagcg gcctttattt catctttggt cagacagagc
    15661 aaatcagatt ccttgcggtt gaaattgaag gcaaaatcct gaagtttttt cttattatca
    15721 tctaccagcg tgtaaaatcc ggcttctttt atcaggttgt ttacgtgcat caccaagtgc
    15781 gactggatgg tcttttgttc ggggatgatg gatgttttgt cgttttcaag tctgaatacc
    15841 ttatccgatt tgtcggccgt attcatgtca gactcaatat aggcatcttt gtcgagggta
    15901 taggctattt ttgatgcttt ggtagtcgaa atgctcattt tgtacaacat cggtatgaat
    15961 acctcggcac tatttacaag gtcattgtat tgttgatcta atggtgcaaa gcacaaatat
    16021 tggttgcctt tttctacctt gtatttggct agataagttg aaccatcgcg gttggtgagt
    16081 agttttacaa cagctttgcc tgcaaattct tgtataggaa atgaacctgt ggtagctggt
    16141 aatttcaagt tagcttttgc attttcgtaa acatcactaa acacaaaatc gttggtattg
    16201 atgcttgcta cctttctctc agtttttgaa aaagcctgaa gctggtgggc ttggatggaa
    16261 ttgaaatacg cattatatga ggcaagatcg gaattttcgg ccgggaaaat caatacattt
    16321 ccaccatttt tcatataatt gactaattcc tgagccaaac cagagcttat agacttgagt
    16381 tcgttgagta tgatgagttt gttgtttcca agttttgaat aatcaatggt cttgttgttc
    16441 actgaattta gcctaaaata actgatccct ttgaatgcag cctccagata acgattcggc
    16501 aagtcttcat ttatacaaag cacatcaatt tgctcagcaa cattaaatgt aaaataataa
    16561 tgatcgtcaa actgtattgg ataatcagtt atgaccagtt cggcctgatg ccagccggtc
    16621 ttatttatgg caacgtgtac cgtatctgta acggttgcat tggcggcgat attcaggttg
    16681 ccta
//
LOCUS       DAOQQY010000029        16572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1086_length_16572_cov_7.254889, whole genome shotgun sequence.
ACCESSION   DAOQQY010000029 DAOQQY010000000
VERSION     DAOQQY010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16572
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1086_length_16572_cov_7.254889"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="PLE23_02985"
     CDS             <1..1372
                     /locus_tag="PLE23_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82261.1"
                     /translation="IENSGDSISLQIDANGEMRFKYSGNVVSRTTDEIYSAIPAIPAL
                     VTDTFYGVPVKLNNNITIKKADVSAGTVFFTYKSYHTQNVTVKITIPELKKNGVAWST
                     SRYLPYTGNTPITDVILPISVAGYSLDIPNDTMHIRYEAVKQDGQRDTLSSFAMLVNG
                     LKFSYMEGYFGNEVFPISRDTIHLNIYKNLLGGELYFEDPKVTVNVFNSFGFPVRSKV
                     NIVKFKGSKGEEYDLESPYVTNGFDFGYPKITEKGKTVITSFSFDKNNSNIVPILNSS
                     PVALDYQIDAIANPDSDPSIIGFMTDSSYFLVNVAVDLPIYGRAKNFLLEEDFSSDFG
                     KLDGFREAEFKLLTENKLPLDATMQMYFLDANGQKLDSAFTQEQLLIEAAKVDATGIP
                     TGVSKKETIIQFNEAQLNAVRKTKTIRVVAYVSTTSGGTVPVHINASQGLSVKMGAKA
                     KLTNKL"
     gene            1378..2694
                     /locus_tag="PLE23_02990"
     CDS             1378..2694
                     /locus_tag="PLE23_02990"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HMX82262.1"
                     /translation="MKRLLIICLTLLSCVPFLRSQSAYRYLLDDADGNIFKQESGVLP
                     VNKFSITAGDYSLGVLSGGISYNNFIKSETENLIEIDAQNVYNDLQDANLLSFQTELK
                     TLGISYRLGKVGVGFQHSITAFNYFTYPKELFGTLFLGNAQYIGKTIPLGPELNSALM
                     NSFGFGIGAELSRVKIAAKLNLLTGLAGAHTNRTKLDLYTDPEYYQLRLTTDYEAATS
                     GLLNLDSLKSSKILATLGNYKLSDAFSSNFGLSVDLSVHARLSEKLSGGVSLKRLGYI
                     DFNKNTNIYSSNKVISYDGLDLSKYITKDSVAVEGLLDSLQDLVKFDKKQGSFKMKLA
                     PEVQLYAKYHLNEKLGLFGGVYFSSVPGKSIWSASGGVSYTPVSLITLGSNITYINGS
                     VLNIGLHGMLNIWKVSLQAGSDNILAAISPKNATFATVYAGVKFNF"
     gene            2713..3951
                     /locus_tag="PLE23_02995"
     CDS             2713..3951
                     /locus_tag="PLE23_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HMX82263.1"
                     /translation="MMKNFYTLILLFCFGFNAVAQVNVPGCDGTRYIEDVFPTVTKTT
                     VTYGYNINTLGQNQELKMDVYTPDGDTLSNRPVIIWAFGGSFIVGQRADMAQHCIAFA
                     KKGYVTATIDYRLWPLLQKGFPTGEQILGVVVQAVSDMKGSIRYFRKDADQGNQFGID
                     PARIIVGGYSAGSVTALHVAHLDPQDSLPPLISNAINANGGMNGNTGDSLMLTYPTDV
                     LSCINLSGGLYEADWIDANSPAFVSYHGDADGTVPYESGIANGLLYIEGSKLLKERSD
                     AVGVNNYFVTVPGGGHTDSYTDQKFASYFNEFWTNTFSFLEDLVCGASTSTTEPNGVK
                     EIISSPNPANNFVKIDLPSAGNYTVELINTIGKVNRILNVNNNNQLTIERENLPAGMY
                     FIKINSTTGINQYVSRVVFY"
     gene            4053..4625
                     /locus_tag="PLE23_03000"
     CDS             4053..4625
                     /locus_tag="PLE23_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HMX82264.1"
                     /translation="MERKIIDEIIRNRRSVFPEMYEDREIDDETILNIIENATWAPNH
                     KLTEPWHFVVFKKNSLEKLSNFLGKHYKESTPEEKFSEMKYKKILSKPLKSACMVAVC
                     YRKGPQNRVPEQEELLAMGAAIENFWLTCSAYGIGCYLSTTSAINHIGELADLQEGER
                     CVGLFYMGWRKPCELTGTRSDYRQKISWIS"
     gene            4716..6965
                     /locus_tag="PLE23_03005"
     CDS             4716..6965
                     /locus_tag="PLE23_03005"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HMX82265.1"
                     /translation="MESLVYLLPLFGVLGLVYMFILFGWVSKQDAGSARMSEISTHIA
                     EGAMAFLKAEYRILAIFVVLAGAALGVLSQMVSTSHWFIVIAFIIGAVFSAVAGNIGM
                     RIATKANVRTTQAARTSLSSALNVSFKGGAVMGLGVAGLAVFGLSALFAFLFFWLGGG
                     TWPGYEKMTIILEALAGFSLGAESIALFARVGGGIYTKAADVGADLVGKVEAGIPEDD
                     PRNPATIADNVGDNVGDVAGMGADLFGSYVATVLASMVLGNYVIKDMGGSISDSFGGL
                     GPILLPVVIAGIGIIFSIIGTFFVKVSDKDANESAVQKALNIGNWASIILTLVASYFI
                     IQWMLPAEMTMNIFDEGKFTPATVTSMDVFFAVIIGLIVGAVISSITEYYTGLGKKPV
                     VDIVKQSATGAATNIIAGLATGMMSTALPVILFAFAIWGAYSFAGFYGVAIAASAMMA
                     TTAMQLAIDAFGPIADNAGGIAEMSELPKEVRQRTDILDSVGNTTAAIGKGFAIASAA
                     LTALALFAAYVTFTGIQGINIFNAKVLAALFIGGMIPVVFSALAMSAVGRAAMDMVNE
                     VRRQFREIPGIMEGTGKPEYGKCVEISTKAALREMMMPGAITIITPILIGFIMGPEAL
                     GGYMAGVTVSGVMWAIFQSNAGGAWDNAKKSFEKGIEINGKMEYKGSDAHKAAVTGDT
                     VGDPFKDTSGPSMNILIKLTCLVGLAIAPILGERAGHSHSDNNSKNKIENTLGYNTPA
                     DTEDVYNIK"
     gene            7108..8811
                     /locus_tag="PLE23_03010"
     CDS             7108..8811
                     /locus_tag="PLE23_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82266.1"
                     /translation="MTVIRLLPFWIMFQLLYFNAYATIIVPFRDLGEVLQAADLVVYG
                     EVEDDFMYHQEENSQQLFIVNPLLILKGDYHLNEKITIKNYHRKYQELEAKVWGDLNP
                     ESGKKYLLFLKHSNQPGMYTALCMSYYCFEETVKDEKSYLVPVAESTELHLHERPDGQ
                     TPEPLRAYLKSDLLKFLYNLKDNPGKWKEGAKVLVTLPPDDIIPRVDPPSHCTFIFAP
                     NLSRWQDFPGTPLPVSYRAGGDAQCSNAVTYTQDAVTVLNSNYLGIKLDNNGPFSGYT
                     PNCTGGSASGGNFTSYCNSNLGGARNVCVIYNDPCSEIPDLSGCAGILAIGGHYAGGS
                     HSFQGKTWLNAMYGYVIINEIGSCYCSAGVYQIMLEHELTHTLGIGHIAPSYGDANMN
                     PSCCKNISTLDVDCLDYTYPASMPVTLVSFSGKLDNNEIFLLWETAEELNNQEYIIEQ
                     STDGIMFDRVRTVVAAGTSSHANRYELAIPALPGQNYIRLSQVDFDGTLSYIGNILSF
                     NNKTFSDNTMKAWYSAGQLVIYPVSNVHNDEYKIEIYNAVGIPVRSVNVLSEKILNID
                     V"
     gene            complement(9086..9307)
                     /locus_tag="PLE23_03015"
     CDS             complement(9086..9307)
                     /locus_tag="PLE23_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82267.1"
                     /translation="MKAILDDWKLIALACITLGLAPFYPEPHIWGKLKWVLGGAHGMQ
                     LIDWWDFIMHGAPWFLLIVYLIRKFLIRK"
     gene            complement(9304..10173)
                     /locus_tag="PLE23_03020"
     CDS             complement(9304..10173)
                     /locus_tag="PLE23_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMX82268.1"
                     /translation="MEHYFKIIWDSYYGYANYLKNELLYPGWHNYLYWLIGISLVVWL
                     LEIIIPWRKKQPVFRKDFWLDGFYMFFNYFLFSLIAYNAISNIGVDLFNRFFGIFGIT
                     NLVAIEISSWPSWAQFLTMFLVADFIQWSTHVLLHRVPFLWEFHKVHHSVEQMGFAAH
                     LRYHWMETIVYKTIQYVPLAMIGFGLKDFFILHIITVTIGHLNHSNLNITYGPLKYIL
                     NNPAMHIWHHAKEIPNKYGVNFGISLSLWDYLFKTAYLPKSGRDIELGFDNVDKFPKT
                     FISQVVYPFKKQS"
     gene            complement(10183..10482)
                     /gene="trxA"
                     /locus_tag="PLE23_03025"
     CDS             complement(10183..10482)
                     /gene="trxA"
                     /locus_tag="PLE23_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMX82269.1"
                     /translation="MQEKFGDIINSEKPVLIDFSAEWCGPCKMQAPILKQVAAKVGDK
                     ARILKIDVDKNPSLAQAYKIQGVPTLIIFKNGEIKWRASGVKMADELTNLLLAEV"
     gene            complement(10490..10945)
                     /locus_tag="PLE23_03030"
     CDS             complement(10490..10945)
                     /locus_tag="PLE23_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX82270.1"
                     /translation="MKKTLLNTFMLSLLFMGSAVLFTSCAQNTTQENQGTTAPQSNAA
                     PTATATVQDIDATTFIEKMKEPNTVILDVRTPEEYSAGHIPNSVLIDFTAPDFSTKIK
                     DLDKSKTYLVYCAAGSRSKAAGREMVKNNFQSVYNMLGGFMNYHGPSEK"
     gene            complement(11066..13378)
                     /locus_tag="PLE23_03035"
     CDS             complement(11066..13378)
                     /locus_tag="PLE23_03035"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMX82271.1"
                     /translation="MSIKANLTAFVCLILSLGVYAQQQKVFIEFFPAAGFYSDSIVVE
                     LSQPGAAIYYTLDGSTPSLKSKKYTKPIQIKRSTVIRAMSFIHGKRSDINAQTYFINE
                     PASTLMVVSVAIPPGILFNKKYGLFMTGTNVDSSNWKLPTANFWSRTETLCNIECFET
                     DGNQVINQLSGFRIFGGMSRLFPQKSFAVAAREKYGVKKFDYPFFGKKAPEKFKFLVF
                     RNSGSDWGKSHFRDALMTSLVSKWDMDVQAYRPAHVYINGKYWGIYNIREKINRHFLS
                     SHHGLHKDSVNLMEHKFDLKFGNARSYYKMMRFIQTADFRNDKNMAILNRMMDVDNFM
                     NYQIAQIYFDNQDAGGNIRYWKPKQHNGRWKWILFDTDFGFGLHNPDAYRMNTLAIHT
                     DPNSKGWPNPPWSTLILRKLLANKSFKQAFLTRFCDHLNDSFAPTRVEKKIDKFYTTL
                     LPEMPRHTNRWQLDFNTWTEQVDIMRLFARQRPLFMRVFLRDMFSPGRECLIHLDVDK
                     GGSIVINNTIETDTSAFDGIYFSSLPVTLRAKADFGYLFDHWEGPGLYSTKDSITVQL
                     SEPVTNYRAVFKPFENKFKDKLVINEISNANPRSGDWIELYNSSLQDINIGGWVFRDS
                     KHEFTLPSYVLKSGNYLVVCQDLLKFRRVFKHITNVIGSFNFGLSKTKESIELYSTDK
                     SMVDKVYYELTPGDSLTTMALIMPQLDNSGTDYWKSLIGIGSPGELNPFVLNSSVEPA
                     DQKWIKMGAWAGLILVLLLGTFWWLSIRKQ"
     gene            13520..14062
                     /locus_tag="PLE23_03040"
     CDS             13520..14062
                     /locus_tag="PLE23_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMX82272.1"
                     /translation="MHRVSAWLLKLFGWKILGDFPLVQKKYVVVVAPHTSNWDFPLGL
                     LVRSARRAKTVFIGKHTLFQFPYGFIFRWLGGYPVDRRKSHNFVESVVKIFDSKEEFT
                     IVISPEGTRRKVEKFKTGFYYIAKGADIPLVLCKFDWGNKEVVFDKPFYTTDDEKKDF
                     ERIYAYFKGVKGKNPELGVD"
     gene            complement(14140..16479)
                     /locus_tag="PLE23_03045"
     CDS             complement(14140..16479)
                     /locus_tag="PLE23_03045"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMX82273.1"
                     /translation="MLLLILCLFAGLDSMFAQQRIVLFEEGTGTWCQYCPRGDVYAQE
                     LQKKYPGKFAFVSIHFSDPMAYAEYGDAMPFTGLPSGWVNRNAITDLLPFSSLQQDIE
                     AQLAIAAPASVSVATDWNATTRVLKMDVTADFTQNLTGDYRLAAIVVENGVTGPAPGY
                     NQTNAYSGGALGKMGGYETLPNPIPASIIAYNHVARYLAGGINGDAGSLPANISTGEV
                     HSYSYTYTLPEAYDFNKVRVIGVLVNATTGEVLNAGYSNYLNGLANASPFFHSEPKTK
                     AFLGTEYKYDIVAHDPDYDAMVISTASTLPPGMTFEDLGSGNARLGGVPTTVGTYPVE
                     LKLTDGNTEVTQSFDILVGENEGDWIQVGQHGINGFKPSVVDLEISNNNNSFLMVSNQ
                     KNSGISVYTLNADTWQKLGNDITGDAFHSCMTLDGDEPVVFSNGLVNKWDGSTWKQIG
                     NKLPGDFYIFPDIIRAGDGSYFVVYFSPADNQTHSYKYDGTSWNFVGDVTDKYTVWNR
                     LKLDNNGSPLLIYGIDGVNISFSQASVWDGTKWDVLGQGHIDTVRTFYDHDITMDKNG
                     NIYAVLTYGSTNQFLNVYKLNTTANNWELTEFNLAGGAIKRCKIEADTAGGLYVAYRD
                     EKNSGKTSVQKYDGQNWSYVGTPGFTETAHEQSLALDNNGIPYVAYADASVSDQANVK
                     KFKKSTTSVTQPKIVEYHLVVSPNPASAEVFVRAQGAVTYQVFNLLGQCVVSGSLTPS
                     EEIGYSLTGVNVSMLNDGIYFISVSGKQGAQSTKLVLKN"
BASE COUNT         4842 a         3231 c         3522 g         4977 t
ORIGIN      
        1 catagagaac agcggtgaca gtatctcact ccagatagat gccaatggcg aaatgcggtt
       61 taaatacagc ggtaatgttg tttctcgtac tacagatgaa atatatagtg ccattcctgc
      121 tattcctgcc ttggttactg atacatttta tggtgtccct gtcaagctga ataacaacat
      181 tacaatcaaa aaagccgacg taagtgcagg tactgtcttt tttacctata aaagctacca
      241 tacacagaac gttacagtaa aaattaccat tcctgaactg aagaagaacg gtgtggcatg
      301 gagtacgagc cgttatttgc cctatacagg caatactcca attaccgacg taatactacc
      361 catatctgtt gccggttatt cgcttgatat cccgaatgat actatgcaca tacgctacga
      421 ggccgtcaag caggacggac aacgtgacac cttgtcttca tttgcaatgc tcgtcaatgg
      481 cttgaagttc tcgtatatgg aaggttactt tggtaatgaa gtattcccga tatcacgtga
      541 caccatccat ttgaatattt ataaaaattt gcttggcggc gaactttatt ttgaagaccc
      601 taaggtgact gtcaatgtct ttaattcatt tgggtttccg gtgcgctcta aggtaaatat
      661 tgtaaaattt aaaggaagca agggtgaaga gtacgacctc gaaagccctt atgtgacaaa
      721 tggttttgat tttggatatc cgaaaattac ggagaaaggg aaaaccgtga taacatcttt
      781 ttcatttgat aaaaataatt ccaacattgt accaatactt aactcaagtc cggtagctct
      841 cgattatcag atagatgcaa ttgccaatcc ggattcggac cccagcatta tcggttttat
      901 gacagattcg agttattttc ttgtaaatgt ggctgtagat ctgcctatat acggtcgagc
      961 caaaaatttc ctgcttgaag aagatttttc gtcagacttc ggcaaactcg acgggttccg
     1021 cgaagccgaa tttaaactct taacagaaaa taaactacca ctcgatgcta caatgcagat
     1081 gtacttcctg gatgccaacg gtcaaaaact cgactcggca ttcacccaag aacaattatt
     1141 gatagaggct gctaaagtcg atgctactgg cataccgacc ggtgtcagca aaaaggaaac
     1201 gattatccag ttcaatgagg cacaactcaa tgctgtgcga aaaactaaaa cgatacgtgt
     1261 tgtcgcttat gtttctacta cctccggcgg aacagtgccg gtacacatta atgctagtca
     1321 gggtctgagt gtaaaaatgg gagctaaagc aaagttgaca aataaactgt aatgtgtatg
     1381 aaaagattat tgataatttg tttgacatta ctttcttgtg tacctttttt gaggtcacag
     1441 tctgcataca ggtatttgct tgatgatgcc gatggcaata tttttaagca ggagtcaggc
     1501 gttctgccgg ttaataaatt ttccattact gcaggcgatt actcacttgg cgtactaagc
     1561 ggtggaatta gctataacaa tttcataaaa tcagaaacag aaaatctgat tgaaatagat
     1621 gcacagaatg tttataatga cctacaagat gcaaatctgt tatcattcca gacagagtta
     1681 aaaactttag gtatatctta tagacttgga aaagttggcg tagggttcca gcacagtatt
     1741 acagcattca actattttac atatcctaaa gaattattcg gtaccttatt tttgggcaat
     1801 gcccaatata ttggtaagac aataccgcta ggaccggagt taaattctgc acttatgaat
     1861 tcgttcggct ttggtatagg cgctgagtta tcaagggtaa agatcgcagc aaagctaaat
     1921 ctgcttacag ggctcgccgg tgcacacacc aaccgtacca aacttgattt atacaccgac
     1981 cccgaatatt atcagctacg gcttactacc gactatgaag cggccaccag tggtctgtta
     2041 aatcttgatt cattgaaatc cagtaaaatt cttgccacac ttggcaatta caagctgtca
     2101 gatgcattta gttccaattt tggcctgtcg gttgatctgt ctgttcatgc cagacttagt
     2161 gaaaaactaa gtggtggggt aagcttgaaa cgacttggat atatagattt caataaaaat
     2221 acaaacattt attcttctaa taaggtaatc agctacgacg gtctcgacct cagcaaatac
     2281 attacaaaag attcagttgc tgttgaaggt ttacttgact cacttcagga cttggtcaaa
     2341 tttgataaaa agcaaggatc atttaagatg aaactggcgc cagaagttca gctttatgcc
     2401 aaataccatc tcaatgaaaa gcttggcttg tttggagggg tatatttttc atctgtaccg
     2461 gggaagagta tctggtcagc cagtggtggt gtaagttata ctccggtttc attgatcaca
     2521 ttgggtagca atattactta tatcaacggt tctgtattga atattggctt acacggtatg
     2581 cttaatatct ggaaagtctc attgcaggct ggttctgata atattttagc agccattagt
     2641 ccgaaaaatg ctacatttgc tacagtctat gctggcgtaa aattcaattt ttaaccatat
     2701 aaaaaacccg atatgatgaa aaatttttac acactgattt tgttattttg ctttggcttc
     2761 aatgcggtgg cacaagtaaa tgtgccgggt tgtgatggta caaggtatat tgaggatgtt
     2821 ttcccaacag ttacaaaaac aacagtcact tatggataca atatcaatac acttggccag
     2881 aaccaagagt taaaaatgga tgtttataca cccgatggtg acacactttc aaatcgacca
     2941 gtaatcattt gggcttttgg aggcagtttt atagttggtc agcgggctga tatggcacag
     3001 cactgcatag cttttgctaa gaaaggttat gtaacagcta ccatcgatta ccgtttatgg
     3061 ccattgctcc aaaaaggttt ccctactggt gagcagatac ttggtgtcgt cgtacaggca
     3121 gtgagtgata tgaaaggatc catcagatac tttagaaagg atgcagacca gggcaatcag
     3181 tttggaatag atcctgcacg cattatagtt ggcggttatt cggccggatc ggtaactgcg
     3241 cttcacgtag cacatcttga cccacaggac tcattgccac cacttatctc taatgccatt
     3301 aatgcaaatg gaggtatgaa cggaaataca ggtgactcct tgatgctgac ttatcctacc
     3361 gacgtgttgt catgtatcaa tttgtctggc ggactctatg aagccgactg gattgatgcc
     3421 aattcgccgg cttttgtgag ttatcatgga gatgctgatg gtactgtacc ttacgaaagt
     3481 ggtatagcca acggccttct gtatatagag ggaagcaaat tgctaaaaga aagatcagat
     3541 gcggtaggtg ttaataatta ctttgtaaca gtacccggtg gcggacatac agatagctac
     3601 acagatcaga aatttgccag ttattttaat gaattttgga ccaatacctt tagctttctc
     3661 gaagatctgg tttgtggagc aagcacatca actactgagc caaacggtgt gaaggaaatt
     3721 atttcaagcc ccaatccggc gaataatttt gtgaaaattg atttgccatc tgcaggtaac
     3781 tatacagtcg agctgatcaa tacaatagga aaggtgaata ggattcttaa cgtaaacaac
     3841 aataatcagc tgacaatcga gcgtgaaaat ttgcctgcag gaatgtattt cataaaaata
     3901 aatagcacaa caggcataaa tcaatatgta tcgcgtgtag tgttttatta atcgtgttaa
     3961 gcaactgaga ataattataa gtctttaaag cagaaatgcc ggatgttgaa cattcccggc
     4021 gtttttgttt tattaaaaat ttttttatta aaatggaaag aaaaataatt gatgaaatca
     4081 tcagaaaccg caggtcggtt tttcctgaaa tgtatgaaga ccgcgagata gatgatgaaa
     4141 caatactcaa catcatcgaa aatgcaacct gggctcccaa tcacaaactt acagaaccat
     4201 ggcactttgt tgtttttaag aaaaattcgc tggagaaatt atcaaatttc cttggtaagc
     4261 attataaaga aagcactccg gaagagaaat tttccgaaat gaagtacaaa aaaatattgt
     4321 ccaaaccact gaagagcgcc tgcatggtcg cagtttgtta tcgaaagggt ccacagaaca
     4381 gagttcccga acaagaagaa ctgttggcga tgggcgcagc aattgagaat ttctggctca
     4441 cttgctcagc ttatggcatc gggtgttatt tgagtacgac atcagccata aaccatattg
     4501 gcgaactggc agatctgcaa gaaggtgaaa gatgtgtagg tttgttctat atgggatgga
     4561 gaaaaccctg cgaactgacc ggtacacgct cagattacag acaaaagata agctggataa
     4621 gctaatacac cgcagtaatt gtattttagc aaaaattgaa ttacttttgc acgcaatttt
     4681 aaaattatca atttaagaca gacaaacaaa acagtatgga atcattagtt tatttattac
     4741 cgctttttgg tgtccttggc cttgtgtaca tgttcatctt gttcggatgg gttagcaaac
     4801 aggatgcagg aagtgcgaga atgtcagaaa tatccacgca cattgccgaa ggcgcaatgg
     4861 ctttccttaa ggcagaatat agaattctgg caatatttgt agtattggcg ggtgctgcac
     4921 tgggtgtatt atcccagatg gtcagcacat cgcactggtt tattgttatt gcatttatta
     4981 taggtgcggt gttttcggct gtagccggta acatcggaat gagaattgct accaaagcca
     5041 atgtgagaac cactcaagcg gcgaggacca gtctgtcatc tgcattgaat gtttcattca
     5101 aaggaggtgc agtaatgggt cttggtgttg ccggtctggc tgtatttggt ctgagtgcat
     5161 tatttgcatt cttgtttttc tggttaggtg gtggcacatg gcctggttac gaaaaaatga
     5221 ccatcatact cgaggcactt gccggttttt cacttggcgc cgagtcaata gctctttttg
     5281 caagagtagg tggtggtatc tataccaaag ccgcagacgt aggtgcagac cttgttggta
     5341 aggtagaagc aggtatcccg gaagatgatc ctagaaatcc ggccactatt gcagataatg
     5401 ttggtgacaa tgttggtgac gtagccggta tgggagcaga cctttttggc tcgtatgtag
     5461 caacggtgct tgcatcaatg gtacttggca actatgtaat taaagatatg ggtggaagca
     5521 ttagcgatag ttttggcgga ttaggcccta tattattgcc tgtggtaatt gccggcatcg
     5581 gtatcatttt ctccatcatc ggcactttct ttgtaaaagt aagtgataaa gacgctaacg
     5641 aatctgctgt acaaaaggca ttgaatattg gtaactgggc tagtataata ctaactcttg
     5701 tagcatcata tttcattata cagtggatgc tgcctgctga aatgactatg aatatctttg
     5761 atgaaggaaa attcacacct gctaccgtta cttcaatgga tgtgtttttc gctgtcatta
     5821 tcggtctgat agtaggtgct gttatatctt ccattactga atattatacc ggcctgggta
     5881 agaaaccggt tgtagatatt gtaaaacagt cagcaacagg tgctgctacc aatatcattg
     5941 caggtcttgc taccggaatg atgtcaactg cattgccggt aatacttttt gcttttgcta
     6001 tctggggagc ctatagtttt gccggatttt acggtgtggc tatcgctgca agtgccatga
     6061 tggctactac agctatgcag ctggcaattg atgctttcgg ccctattgcc gacaatgctg
     6121 gtggtattgc tgaaatgagt gaactaccta aagaagtaag acaacgcaca gatatcctcg
     6181 attcggtagg taatactact gctgctatcg gtaagggttt tgctatcgct tctgctgctc
     6241 tgacagctct tgcacttttc gcagcttatg taacattcac gggtatccag ggtatcaata
     6301 tttttaatgc caaagtgctg gccgctttat ttataggtgg tatgatccct gtggtgttct
     6361 cggctctcgc tatgagtgcc gtcggccgtg ctgcaatgga tatggtgaat gaagtaagaa
     6421 gacagttcag agaaatacct ggtatcatgg aaggaaccgg taaacctgaa tacggaaaat
     6481 gtgttgaaat atctactaag gctgccctac gtgaaatgat gatgccgggc gcaataacaa
     6541 taataactcc gatcttgatt gggttcatta tgggccctga agctctagga ggttatatgg
     6601 cgggtgtgac tgtttcgggt gtaatgtggg ctatcttcca gtctaatgcc ggtggtgcat
     6661 gggacaatgc caagaaatct ttcgaaaaag gtattgaaat caatggcaaa atggaataca
     6721 aaggctcgga tgctcacaaa gctgccgtta caggtgatac agtgggtgac ccgttcaagg
     6781 atacttccgg cccgtcaatg aatatcctta tcaaactgac atgtctggta ggtcttgcca
     6841 tagcaccgat cttaggcgag agagccggac attcacattc ggacaacaac tccaagaata
     6901 agatcgaaaa tacgcttggg tacaatactc cggctgacac agaagatgtg tacaacataa
     6961 aataacagaa tattttctta tatctgcatc aagagagcct tttgttaaga cagaaggctc
     7021 tcttttttta ttgcatttgg taaaaaccat aagtatattt gtcttgtagt agcattagta
     7081 ttccattacc ccaaaaaact taacaccatg accgtaataa gattattgcc gttctggata
     7141 atgttccagt tgctttattt caatgcgtat gccactatca ttgtcccttt tcgcgatttg
     7201 ggtgaagtgt tgcaggctgc tgaccttgta gtatatggtg aagtagaaga tgatttcatg
     7261 tatcatcagg aagaaaattc gcaacagtta tttattgtta atccactatt gatactcaag
     7321 ggagactacc atttaaatga aaaaataact ataaaaaatt atcaccggaa atatcaggag
     7381 cttgaagcca aggtttgggg cgatctgaat cccgaatcag gaaagaagta cctgctattt
     7441 ctcaaacaca gcaatcaacc cgggatgtac actgccttgt gtatgtccta ttattgtttt
     7501 gaggagacgg taaaggacga aaaatcatac ctggtacctg tagctgaaag tacggagttg
     7561 catttgcacg aaagacctga tggacaaaca cctgaaccat tgcgcgccta tctcaaatca
     7621 gatttgttaa aatttcttta taatcttaaa gacaatcccg gtaaatggaa ggaaggagca
     7681 aaggtactgg taacgctgcc gcccgatgac attataccac gggttgatcc gccttcgcat
     7741 tgtacgttta tttttgcccc aaatctgtct cgctggcagg actttccggg cacaccactt
     7801 ccggtctcat atcgtgctgg tggcgatgca caatgtagca atgctgttac ttatacacag
     7861 gatgctgtaa cagtacttaa ttcgaactat ttaggcatta aacttgataa taacggacca
     7921 ttttcaggct atacgccaaa ctgtacaggg ggaagtgcct ccggtggaaa ttttactagt
     7981 tattgcaata gtaacctggg aggtgcccgt aacgtttgcg taatatacaa tgacccatgc
     8041 agtgaaatac ccgacctgtc aggatgtgca ggtattctag ctattggcgg gcattatgcg
     8101 ggtggcagtc acagctttca gggaaaaact tggctaaatg ctatgtatgg ctatgttatt
     8161 atcaatgaaa taggcagttg ttattgctcg gctggtgtat atcagataat gcttgagcat
     8221 gaactgacac atacgcttgg tatcggtcat attgctccaa gctatggtga tgctaatatg
     8281 aatccttcat gttgtaaaaa tatttctaca cttgatgttg attgtcttga ctatacttat
     8341 cctgcaagta tgccggtaac gcttgtgtct tttagcggaa aacttgacaa taacgaaata
     8401 tttctcttat gggagactgc tgaagaactg aacaatcaag aatatatcat cgaacaaagc
     8461 actgatggca ttatgtttga cagagtgcgt acggtagtag cagcaggaac atcatctcat
     8521 gccaacagat acgaacttgc tattccggca ttgcccggac agaactatat aagactcagt
     8581 caagtcgatt ttgacggaac gttaagttat attggaaata tattgtcatt taataataaa
     8641 acattcagtg ataataccat gaaggcatgg tattcggctg gtcagctggt tatttatcct
     8701 gtttctaatg tccataatga tgaatacaaa atagagattt ataatgctgt aggaattcct
     8761 gtacggtctg tgaacgtttt atccgaaaaa atactaaata tcgatgtatg agatttgccg
     8821 gttggcattt atttggcaaa tatcaatggt ctaagagcag taaaattcat aaggatggat
     8881 taacccagat aacacattag gctctttact ccaaatccat tacaaaagcc caaaaccatg
     8941 ggtcactatg cttttaacgc tctattatgg attttttata ctaatcattt tcgtttttaa
     9001 gtcctaatgc ggtatctggc attaactgat taaaaaaact ccggttatct aatcggacaa
     9061 taaccggtgt ttatattctt tataattatt ttctaatcaa aaactttctt atcagatata
     9121 cgatcagtaa aaaccagggc gctccgtgca taatgaagtc ccaccagtca atgagttgca
     9181 taccgtgagc accgccgagc acccatttca gctttcccca aatgtgtggc tccggataga
     9241 atggtgccaa gcccagcgtt atacaagcaa gggcgattaa tttccagtca tcgagtattg
     9301 ccttcatgat tgttttttaa acggataaac aacttgagaa ataaatgttt ttgggaattt
     9361 gtccacattg tcaaaaccga gttcgatgtc gcgaccgctc ttaggcagat aagcagtttt
     9421 gaataggtag tcccacagac taagcgatat accgaagttg acaccatatt tatttggtat
     9481 ttcttttgca tgatgccaga tgtgcatagc aggattgttc agtatgtatt tcagcggacc
     9541 ataggtgatg tttaggttgc tgtggtttag gtgccctatg gtaacggtga tgatgtgtag
     9601 gatgaaaaaa tccttcagcc caaacccaat cattgccaat ggtacatatt gaatcgtttt
     9661 atagacgatg gtttccatcc agtggtacct gagatgcgct gcaaagccca tttgttcgac
     9721 actatgatgt actttgtgga attcccacaa gaacggaacc ctgtgaagta atacatgtgt
     9781 actccattgt ataaaatcag ctacgaggaa catggtcaaa aactgtgccc aggaaggcca
     9841 tgaagagatt tcgatggcca cgagattcgt aatgccgaat attccaaaaa atctgttgaa
     9901 cagatcgacc ccgatattag aaatggcatt gtaggcgatg agcgaaaaca ggaaataatt
     9961 aaaaaacata taaaacccgt caagccaaaa atccttgcgg aaaacaggct gctttttacg
    10021 ccacggaatg atgatttcta gtagccaaac tacaagcgaa ataccgataa gccagtaaag
    10081 atagttgtgc caccccggat acagcaattc attcttcagg taattcgcat agccgtaata
    10141 cgaatcccaa ataattttga aataatgttc cataaatttc tgttagactt cagccaaaag
    10201 aagattagtg agttcgtctg ccatttttac acccgatgca cgccacttta tttcaccatt
    10261 tttaaagatg ataagtgtag gtacgccttg tatcttgtaa gcttgggcta aggaagggtt
    10321 cttatctaca tctattttaa ggattctggc tttgtcacct acttttgctg ctacctgttt
    10381 cagtatggga gcctgcattt tgcatggccc acaccattca gccgaaaaat caatcaatac
    10441 cggtttttca ctgttgataa tgtctccgaa tttctcctgc atatttttat tacttttctg
    10501 acgggccgtg ataattcata aatcctccca gcatgttgta aacactttgg aagttgttct
    10561 taaccatctc tcgtccggct gccttacttc tggaccccgc agcacaatac acaagatagg
    10621 ttttgctttt gtccaaatct ttgatttttg tactgaagtc aggtgctgtg aagtcaatca
    10681 atacggagtt cgggatatgt ccagcactat attcttcggg tgttcttaca tccaggataa
    10741 cggtattagg ttctttcatt ttttcgataa atgttgttgc atctatatcc tgtacagtag
    10801 ctgttgctgt tggggcagca tttgattgtg gtgcagtagt tccctgattt tcctgtgtgg
    10861 tattctgggc gcaactggtg aatagtactg cacttcccat gaagagaagg ctaagcataa
    10921 atgtgttaag cagagttttt ttcatttttt ttatttatga attttataca aaaatactac
    10981 aagatcaggt gacgaggctg taacaaaagt tacttataca gcatgtctat ataataggta
    11041 aagttaatcg tttcttacta aaatattatt gttttcttat acttagccac cagaatgtac
    11101 ccagcaagag tacaagtata agtccagccc aagcacccat ttttatccat ttttgatcag
    11161 ccggctccac actgctgttt aatacaaaag gatttaattc acccggcgaa ccgataccga
    11221 ttaagctttt ccaataatcg gtaccggagt tgtccaactg tggcatgatg agagccatgg
    11281 tagtcaggct gtctcccgga gtcagttcgt aataaacctt gtcaaccatt gatttgtctg
    11341 tactgtacaa ttcgattgat tcttttgttt tactgagtcc aaaattaaat gaacctatca
    11401 cgttggttat atgcttgaaa acccgcctga acttcagtaa atcctgacat acaacgagat
    11461 aattaccgga tttcaataca taggaaggta gggtaaattc gtgcttcgaa tcgcgaaata
    11521 cccagcctcc aatatttatg tcttgtaatg atgaattgta cagttctatc cagtcacccg
    11581 atcgaggatt ggcattgctt atttcgttta ttacgagttt gtccttgaat ttattttcaa
    11641 aaggcttgaa tactgcccgg taattagtca caggttctga gagttgtaca gtaatgctat
    11701 ctttagttga ataaagcccc ggaccttccc aatggtcgaa gagataacca aaatcggctt
    11761 ttgccctaag tgttacaggc aatgaagaaa agtaaatacc atcaaaagcg cttgtatctg
    11821 tctcaatcgt gttattaatg acaatgctgc cccctttgtc cacatcaagg tgaatgaggc
    11881 attcacgacc ggggctgaac atatcacgta gaaagaccct cataaacaaa ggtctttgcc
    11941 tggcgaataa acgcataatg tcaacttgct cagtccaagt attgaagtca agttgccatc
    12001 ggtttgtatg tcggggcatc tctggaagta aggtagtata aaacttatct atcttctttt
    12061 ccactcttgt cggtgcaaag ctgtcattga gatgatcgca gaatctggtc aggaatgctt
    12121 gcttgaatga cttattggcg agcagttttc ggagtatcag cgtactccat ggtggattag
    12181 gccagccttt gctattgggg tcggtatgta tggccaaggt attcatccta taagcgtcag
    12241 ggttgtgcaa accaaaaccg aagtcagtat caaacagaat ccatttccat cttccattgt
    12301 gctgtttggg tttccaatag cggatatttc ctccggcatc ctgattatca aaatagatct
    12361 gtgctatctg ataattcata aaattgtcaa catccatcat tctattaaga atggccatgt
    12421 ttttatcatt cctgaaatca gcagtttgta taaagcgcat cattttatag taagaccgtg
    12481 cattgccgaa tttcaggtca aatttgtgtt ccatcaagtt aacagaatct ttgtgaagtc
    12541 catgatgcga agacaaaaaa tgccggttga ttttttcacg gatgttgtat ataccccaat
    12601 atttaccgtt gatataaaca tgtgctggcc tgtatgcctg aacatccata tcccatttag
    12661 aaacgagtga ggtcattaga gcatcgcgaa aatggctttt cccccaatcg cttccactgt
    12721 tgcggaatac caggaattta aacttttccg gtgctttttt tccaaagaat ggataatcaa
    12781 attttttaac accatatttt tctctggctg ctaccgcaaa cgatttttga ggaaacagcc
    12841 tgctcatacc tccaaaaatc cggaatcctg aaagttggtt gataacctga tttccgtcgg
    12901 tttcgaagca ttcaatgtta cataaagttt cggttcttga ccaaaaatta gccgttggta
    12961 gtttccagtt gctgctgtca acattcgtac cggtcatgaa caggccgtat tttttattaa
    13021 atagaatacc tggtggaata gctaccgaaa ctaccatcag tgtactggca ggttcgttga
    13081 tgaagtaagt ttgtgcattg atgtcgcttc gtttgccatg gatgaatgac attgccctga
    13141 tgacagttga gcgcttaatc tggatcggtt tggtgtattt ttttgatttc agagaaggag
    13201 tagaaccgtc taaagtgtaa tagatggctg cacccggttg gctgagctct accacaatac
    13261 tatctgaata aaagcctgct gcaggaaaaa actcaataaa aactttttgt tgctgggcat
    13321 acacacccaa cgacagaatc aaacacacga aggcggtcag attagctttg atagacatta
    13381 attgaaaata ctacaaatga aaaattcaat ctgagcacaa ttaagtatat tattacggtt
    13441 aaaaaaaact tatggtatgt ttttttgcaa atattatttc acttcatgac aaatattgct
    13501 ttaatttgaa ataaaaatca tgcacagagt ttctgcttgg ttattaaagt tatttggctg
    13561 gaagatcctt ggcgactttc ctttagttca aaagaaatat gtagtggtag tggcacctca
    13621 tacttctaac tgggattttc cgcttggtct gcttgtgcgc tcagccagga gagcaaagac
    13681 ggtattcatt ggcaagcata ctctttttca gtttccgtac ggatttatat tcaggtggtt
    13741 gggtggttat cctgttgaca gaagaaaaag ccataatttt gtagaatctg tagtaaagat
    13801 atttgacagc aaagaagagt ttactattgt tatttcacct gaaggaacac gcaggaaagt
    13861 agaaaaattc aaaaccggat tttattatat cgccaaagga gccgatatac cattggtgct
    13921 ttgcaaattt gactggggca ataaagaagt agtatttgac aaaccgtttt acacaaccga
    13981 tgacgaaaaa aaggattttg agaggattta tgcttatttt aaaggtgtca agggtaagaa
    14041 tcccgaactt ggggtcgatt gaatgtcatt tgagcgacaa taaacaaaca agcccgattc
    14101 taagaccggg cttgtagttt gtacctcgta gtgcttgtat taatttttta gaaccaattt
    14161 tgtgctttgc gcaccctgtt ttccgctaac agaaataaaa taaataccat cattaagcat
    14221 tgatacatta acgcctgtaa ggctatatcc aatttcttca gaaggagtaa ggctgccgga
    14281 aaccacacac tgaccaagca ggttaaatac ctgataagtg acagcaccct gggcccggac
    14341 aaaaacttca gctgaagccg gattaggact tacgaccaga tgatactcaa ctatcttcgg
    14401 ttgtgtcact gatgttgttg attttttaaa tttcttcaca ttagcctgat cgctgacaga
    14461 agcatctgca taagcgacat agggtattcc attattatca agtgccagtg actgttcgtg
    14521 ggctgtttct gtgaagcccg gagtaccaac gtacgaccag ttttgaccgt catatttttg
    14581 aacgctggtt tttccagaat ttttttcatc gcggtaagcc acatacaatc ctcctgctgt
    14641 gtctgcttct attttacatc ttttgatggc acctcctgca aggttaaatt ctgtaagctc
    14701 ccaattgttg gcagttgtgt ttaatttata cacattcaga aactgattgg tagagccata
    14761 ggttaataca gcataaatat tcccgttttt atccattgtg atgtcatgat cgtagaaagt
    14821 gcgtactgta tcaatatggc cctgcccgag tacatcccat tttgtaccgt cccagacaga
    14881 tgcctgtgaa aatgaaatat tgacaccatc tataccataa ataagaagtg gacttccatt
    14941 gttatcaagt ttgagacggt tccatacagt atatttgtcg gtgacatcgc ctacaaaatt
    15001 ccagctagta ccgtcatatt tatatgaatg tgtctggtta tctgccggcg aaaaataaac
    15061 aacaaaatag ctgccatcac cagcacgaat gatgtctggg aaaatataaa aatcgcccgg
    15121 tagtttatta cctatttgtt tccaggtaga gccatcccat ttgtttacta agccattgct
    15181 gaacacaacg ggttcatcac catcgagtgt catgcaagaa tgaaatgcat caccagtgat
    15241 gtcattgcca agtttttgcc aggtgtcagc gttcagcgta taaactgaaa tgcctgaatt
    15301 tttttgatta cttaccatta aaaatgaatt gttgttgttg ctgatttcaa gatcgacaac
    15361 acttggtttg aatccattga tgccatgctg cccgacctga atccagtcgc cttcattttc
    15421 acctactaat atgtcaaaac tctgagttac ttctgtattg ccatcggtta gttttaattc
    15481 aaccggatag gttcctacgg tggtaggtac accacccaag cgtgcattac cactacccaa
    15541 atcttcgaat gtcatgcctg gtggcaatgt actggctgta gaaataacca ttgcatcata
    15601 atcaggatcg tgagcgacga tatcgtattt gtattctgtg ccgagaaagg ctttcgtttt
    15661 aggttcagaa tggaaaaatg gactggcatt ggcaagacca ttcaaataat tgctgtaacc
    15721 tgcattcaat acttcacctg tggtggcatt taccagtaca cctattacac gaaccttgtt
    15781 gaaatcgtat gcttcaggca aagtgtaggt ataagagtac gagtggactt ctcccgtaga
    15841 aatgttagcc ggaagactgc ctgcatctcc gttaataccg cctgcaagat atctggctac
    15901 atggttatat gcaataattg aagcaggtat tgggttaggt agtgtttcat aacctcccat
    15961 tttgcccagg gcaccgcctg agtacgcatt tgtctggttg tatccgggtg ctggtccggt
    16021 aacaccattt tctaccacga tagcggcaag ccggtagtcg ccggtaaggt tttgggtaaa
    16081 atcggcagta acatccattt tcagtactct ggttgttgca ttccaatcag tagctacact
    16141 taccgatgcc ggtgctgcaa tagcgagctg cgcttcgatg tcttgctgca agctggagaa
    16201 aggcaacaga tcagtgatgg cattgcggtt gacccatcct gaaggaagtc cggtaaatgg
    16261 catggcatca ccatattcag cgtatgccat tgggtcgctg aagtgaattg atacaaatgc
    16321 gaatttgccg gggtattttt tctgtagttc ctgagcatat acatcgcccc ttgggcaata
    16381 ttgacaccag gtacctgtac cttcttcgaa caagacaata cgctgctgtg caaacattga
    16441 atccaatcct gcaaacagac acaaaatgag taaaagcatt ctattcataa tggttttaac
    16501 ttaagtttgg agttatttca atgtcgttta gttaagcgaa agtatttata aaactttgta
    16561 attcattgat tt
//
LOCUS       DAOQQY010000030        16297 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1115_length_16297_cov_5.912511, whole genome shotgun sequence.
ACCESSION   DAOQQY010000030 DAOQQY010000000
VERSION     DAOQQY010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16297
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1115_length_16297_cov_5.912511"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="PLE23_03050"
     CDS             <1..1607
                     /locus_tag="PLE23_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMX82274.1"
                     /translation="YKDQKSAVTEQADRLTGAIAENEKRFISDSRLIKTNDNAAQDLP
                     DFFKKIDEQFDATYGGFGSAPKFPQTQTIDLLLATHYYQKNDEALVQALFTIKKMIRG
                     GIYDQLGGGFARYATDAAWLIPHFEKMLYDNALLVRTMADVYRITKDTEIEHSIHETL
                     AFICKEMTGTEGGFFSAIDADSEGVEGKFYIWKKEEIENVLGDGTQLFCKIYNVTRSG
                     NWEGHNILNLADSVKEYCVKNKLDYHQTTTDLERQKKILLNKRNERVRPGTDDKILLG
                     WNALMLTAYAEAGMALGNKTYITIAVEQHKYIKDMFRRDDGAFNHTYKDGKAKIDAMF
                     DDYAFYIEALIALFHSTQEVSYLYEARELTEYMFGEFYEEVDGMFYFTGAHADNIVRK
                     KDFFDSATPSGNAVHCRNLMYLGVIFDLKKYSDTAERMLNAMDASVRLYPTSFAYWAK
                     NRMIHHYGLEEVVTSGRYKDQFVEQINMIYSAGRILMKTGIGRGDLILLLNKEETEES
                     LIYLCKDYNCQRPVHSIEAFVEMLNK"
     gene            1609..2586
                     /locus_tag="PLE23_03055"
     CDS             1609..2586
                     /locus_tag="PLE23_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMX82275.1"
                     /translation="MRILKVYILILSGMLLLPSIARSQQSTQYSLYHLDKHLFNPAYA
                     GVEPELTATAIVRNQWLGINGSPLYQQLSLNAPLIAGNSGIGLNFENDNLGLEHNINA
                     SVSYSHRFRFSENGFLAAGIRGVWSGKSLDGTKITTPDGKYEPGSSPDHKDGLLPVGK
                     SRGNIFGIGAGILLRAGNFEVGAGVDNIVPGEYKFEAFQIKYDPTWYLNAGWLLPVSD
                     FWQLKLDGLARTDGTLVQTDAVVKILMGNNMFAGSSFRGYSNRTIDAISVFAGIRVME
                     SLTLAASYDLGLSDLRDIHSGSIEFGLRYSYGNKIFREKLPVVIYNPRY"
     gene            2810..4054
                     /locus_tag="PLE23_03060"
     CDS             2810..4054
                     /locus_tag="PLE23_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMX82276.1"
                     /translation="MKKHLRLLPFLTLLLILSSCGRKENGQLIGVLDRPTWKNINPYG
                     MVYIPSGTFTLGQSDEDVRRTYMQRQKAVSIQGFYMDDTEITNNEYRQFVNWVSDSIA
                     HTMMDHMTEDDQGNQTIDWEQQLDWTDETLNDLYYQGDDKFAGKKEINTSKLVYAYEW
                     YDWQAAAHDHGKSPRNKFIKREKIKIYPDTMVWIRDFAYSYNEPFTRNYFWHPAFDDY
                     PVVGVNWHMANAFCYWRTKLWNSYRASKKGEVNSEDFRLPTEYEWEYAARGGRAEAPY
                     PWGAYYTRNAKGCLLANFKPGRGNYPEDGGLYTVKADAYFPNDYGLRNMAGNVAEWTI
                     TAFSENGQSFIHDLNPDIRYDAKADDPIANKRKVIRGGSWKDVAHFIQTSTRHYEFQD
                     TTKSYIGFRCALTFLGRSINDF"
     gene            4221..4778
                     /gene="gldL"
                     /locus_tag="PLE23_03065"
     CDS             4221..4778
                     /gene="gldL"
                     /locus_tag="PLE23_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HMX82277.1"
                     /translation="MKNFIIGVGAAIVLIGALFKLESWPYASEMLITGLSVEAFIFLF
                     LGIIGPEKDYYWEKLYPGLDDYHSHISSITQGEKPAAAVPQLNGQVVENKLGGMLDEL
                     QSMSKSLGSLKALQEADFSGTADQVKAMGNFYAKLNGAMSELAQSVEDTKAYKDNLTA
                     LNKNLGSLNSVYGGMLNAMSNIGKS"
     gene            4839..6398
                     /gene="gldM"
                     /locus_tag="PLE23_03070"
     CDS             4839..6398
                     /gene="gldM"
                     /locus_tag="PLE23_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HMX82278.1"
                     /translation="MSIPKEPRQLMINLMYLVLTALLALNVSAEVMNAFFSLNKGLEN
                     SNKIVDNTNITIKNSIDKQADANKKEVNIKFKNSADAMLSELKDFTAYVDGIKKTVFD
                     AAGGPSKDNPTIPVNIKDKDIPTRLLVYDNAKKKDGMGVELEKKIKEFRDKLIKYADG
                     DQGTIKSISLDTEEVPKGSDKKNWADFRFRQMPVAAVFPILTKIQSDAKTSSAAVLNY
                     INGKVAGEDLKFDNFKVAIAPKTGYVVRGDKFEADVYLAAYSSNPGQGVSISVNGANL
                     GMKDGVAKYTSTPGSIGKQTVRATATIKNPLTGEVKRVDGTFDYEVGERSVAVSADKM
                     NVFYIAVDNPVSVSAAGVSSNKLNVGVSGEGGGSITKAGSTNYIVRVSRPTKPGEFCY
                     ITANADGLSDKKPFRVKRIPDPVARLSNKANGAMGTGEFKAQGGLGAFLDNFDFDAKC
                     AIVGFTLVRVPKRQDAVREANDGARYNGKCRGLIDQAKPGDTYYFEDVKAKCPGDNAG
                     RPINPLVFTIK"
     gene            6411..7268
                     /gene="gldN"
                     /locus_tag="PLE23_03075"
     CDS             6411..7268
                     /gene="gldN"
                     /locus_tag="PLE23_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HMX82279.1"
                     /translation="MKFQLKLFLFIACLGGASVSYAQVPETIKTESSEPGETPLDDVV
                     ERTINVEKRVLKYDNPREVDLMWEKRVWRVIDLREKMNFPFTYPEETFAGILLNGAKE
                     GKIRVYSTKDDKFTDPLTAANLESKLVKIDTIPTVDPETYEETIQIVKNEINENDFKR
                     IRVKEMWYFDSETSTMKVRILGLAAIRDVFDDNGNFKYEEPMFWIYYPHIREELAKHR
                     VFMEGNDASPLTWEDFMEMRFFSSYVYKESNVKDNRLSDYLSGVDLLMEGERIKADIF
                     NWEHDLWQY"
     gene            complement(7365..9284)
                     /locus_tag="PLE23_03080"
     CDS             complement(7365..9284)
                     /locus_tag="PLE23_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HMX82280.1"
                     /translation="MELEEINKHYIELQRLLKLEKEEDFTFHNNQLATLTVKQKIEAG
                     YLWRPVVVTKTGFTMGGRISITVEHKSVTSPNHQLQPGRPVIFYSEGTNDEDNQMKAT
                     VYSVGKNTMKLISNARELPEWLNKPPLAVQAEFDERTYRLMENALEEVINSKEPRVRE
                     FKKIFAGLQPPIFSHETYPFTIPILNESQNQALQHVMQSKDLAIIHGPPGTGKTTTLV
                     QIIKKTVETEKQVLVTAPSNVAVDLLVERLVQAGVKTVRIGNISRIDETLLAHTLDGM
                     VESHREFPNIKKIRIEAAAVRSEASKYKRHFTHTEKEERTRLRKEAKDLEAWAKDLEE
                     RITESILDSAQVIATTLTSCNNDELSGRKYSTIFLDEASQALEPAAWITMLKADRIVM
                     AGDPLQLPPTVKSTKAAKAGLDISLLEKSISLNYPSYLLDTQYRMHNDIMTFSNVYFY
                     KGKLQTALEIQSKTQNQTFKAVIFIDTAGTGFEEQMPEETSKDMYLSKYNEGEYFILR
                     EHLLRLISEHLELKEKNIVVITPYRAQVRYINDRIQEDETLYNLNIRVSSIDGYQGQE
                     SDIVIISLVRSNVKGEIGFLKDYRRMNVAMTRAKLHLAIIGDSATLSGDKFYSTLIDH
                     VNDAGQYHSAYEYMY"
     gene            complement(9266..10612)
                     /locus_tag="PLE23_03085"
     CDS             complement(9266..10612)
                     /locus_tag="PLE23_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82281.1"
                     /translation="MEARPEIKALSHSGNDAREVILLALWSATIIYAGFAVERHQHIQ
                     LFSLWMLSFGCYWYLAVKSTLRLNTVLLSAIAVRFVLIFAFPKLSDDIYRFIWDGRLL
                     VQGINPFEATPARLMESVKLTVVDQQLFNRLNSPEYFSVYPLVCQSVFYVSSYLAGDN
                     IMLNTILIKAFLFFCECATMYFSVRLLKKLSIAPEKIFWYALNPLIVIEICGNAHFEG
                     AMTAFLAAGIFYYLNGNKFLSALTFALSVASKMLTAIFFPLLFFGEIRRRKLKFILLL
                     GIFTAMLSLPVIQNTSIFNSLDLYFRNFEFNAGIYYWLRWAGFKLRGYNQIRFIGPLL
                     SAISFSIIILYSYKKSDNKELLYRMFWLIFIYIIFSTTVHPWYLSLMLFTGIFTRYRF
                     PFVWSGLIFFTYFNYNADTFAENLWIVIAEYLIVMGMLLYEANIFHKIQNLWNSKK"
     gene            complement(10591..11940)
                     /locus_tag="PLE23_03090"
     CDS             complement(10591..11940)
                     /locus_tag="PLE23_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX82282.1"
                     /translation="MNTLTEADLPFVTIQLPVFNEQYVVERLIDNIVKINYPARLLQI
                     QILDDSTDETLTYSSAKVEYYRTRGIDIELIHRTDRTGYKAGALKNGLTTAKGAFIAI
                     FDADFLPDEDFILKCIPYFFQDERYAVVQTRWEYINRDYSLITRLQALPLNVHFSIEQ
                     TARMYAGLYLQFNGTGGIWRRKAIEDAGGWSADTLTEDLDLSIRTQLKGWKIKYIEET
                     GTPSELPVEMNGFKSQQYRWMKGGAECARKLLPAVFKSDLPFTKKMFTTAYLLSSSVF
                     LLVFLTALLSFPAIFLLDQTGYKLQWASVFMVGWALIFPVYLVGNFNPAVRKTFSFKD
                     VLVFVVLFPLLLSLSLGMSLHNAIAVLDGFRGRKSAFIRTPKYNIVGKTDKLKYTTYR
                     SNKMSFSTRMEYFMALYFVIAGTAGILYGNISFLGVQVLLFVGFFSIATLSWKHVQK"
     gene            complement(12080..12787)
                     /locus_tag="PLE23_03095"
     CDS             complement(12080..12787)
                     /locus_tag="PLE23_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX82283.1"
                     /translation="MARAIIDVIIPAYNEELSIAKVIRDIPKDLVRNIIVCNNNSSDA
                     TAVVASTAGAIVADEPSPGYGNACLRGMAYIEALPSDEKPDILVFMDGDYSDYGEEMY
                     ELVKPILDDGMEMVIGSRALGRRERGSMTTPQVFGNWLSTTLIRWLYGYRFTDLGPYR
                     AIRYPSLLALKMADRNFGWTVEMQVKAAKHKLKTTEIAVNYRKRIGTSKVSGTIKGSI
                     SAGYKILWTIGKLWLNN"
     gene            complement(12841..13851)
                     /locus_tag="PLE23_03100"
     CDS             complement(12841..13851)
                     /locus_tag="PLE23_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX82284.1"
                     /translation="MHQNTTTVKHYQLKNNPALATILPEYPGNTYYKGRYISDESRVM
                     PSWKTFFKWMFSFNEQRSEKRRDEYRLPLIKGNEFFHQTDNQLLWLGHATFLIRLQGT
                     TIITDPCLRNLPFKKRKVGLPFKTEEITRLNFILLSHAHRDHLDLPTLKILVKNNPGV
                     RILAGLGTSGLLPKAWHAQTEEAGWYQIFSNTGSLRIVYLPAQHWNRRYMFDLDQQLW
                     GSFLIESDKTSIFFAGDTAFGQHFDKIRFLTKQIDHCIIPIGSYKPAFMMRGLHLNPK
                     EAILAFKMLNGKNFIPMHYGTYDLSDEPLGEPLRMVAESKSRGSFSQNFRPLAVGERY
                     AL"
     gene            complement(14042..15685)
                     /locus_tag="PLE23_03105"
     CDS             complement(14042..15685)
                     /locus_tag="PLE23_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HMX82285.1"
                     /translation="MNKTININLGGRAFVIDEDAYAKLDYYLTSIHDYFKNSEGYEEI
                     TNDIESRMGELFADNLRGRSIISTADVDYAIHTMGSLEDFGVDTAGTQANFNTGQRSG
                     GIKTGRRIFRDPDEKILGGVCAGLSSYFGINDPIWLRIIFLLLFFVAGTGFLFYMILW
                     ILIPKAVTSADRLSMKGEPININNIAKEVEDSFNHFGQRINDIGREFSGKKKNNLNAN
                     EPYAFSGITNFVGELVEALQYIIPKIIKGFIILIGLICMFAIATAGIALLTSGFFLHD
                     YSSLMFGPGMFSKHWLFVTSLILVFLIPAVALIVLGLKTFFQVKTPSGLLKGLGFAWM
                     LFFLLGIFSSVNFARSFKSHAEVSKANEYFAATMPDTLVIDINENETFDSGDFDNIQL
                     GELRTKNGKFYSDNIHIRVLKSDDQNIYWKVNLESNGGSTQEAESLANSILYPIGQDG
                     SRLIFPGYFEIIQGKVWRDQEVRVNVYVPEGKYLKVTSRTENFVYKIEKDPEQESSYE
                     YSGKIWRMGKAGFVNTSPKENTEESEGQHEDTAEPQDTL"
     gene            complement(15682..15993)
                     /locus_tag="PLE23_03110"
     CDS             complement(15682..15993)
                     /locus_tag="PLE23_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HMX82286.1"
                     /translation="MRKGVLELVILAILSREAAYPSDIINRLKESELIVVEGTLYPLL
                     TRLKNSGYLDYSWQESSSGPPRKYYTLTVKGSEFYDSLKESWASLVQSVNQTIQNKTK
                     S"
BASE COUNT         4776 a         3138 c         3509 g         4874 t
ORIGIN      
        1 cctataaaga tcagaaaagt gcagtaaccg aacaagcgga tcggctgaca ggagctattg
       61 ccgaaaatga gaaacgattt atttctgaca gccgattgat aaaaacaaac gacaatgccg
      121 cccaggattt acctgatttt ttcaaaaaaa ttgacgaaca gtttgatgcc acgtatggcg
      181 gattcggtag tgcgccaaaa tttccacaga cacagaccat cgaccttttg cttgccaccc
      241 attattatca gaaaaatgat gaggcacttg ttcaggcatt atttactata aagaaaatga
      301 tacgcggagg tatttacgat cagcttggtg gaggctttgc acgttatgca accgatgcag
      361 cctggctaat accgcatttc gaaaagatgc tttatgataa cgcactgctt gtaaggacaa
      421 tggcagatgt gtatcgcatt acaaaagata cagaaataga acattctata cacgaaaccc
      481 ttgcattcat ctgcaaagaa atgacaggta ctgaaggagg atttttttca gccattgatg
      541 cggactcaga aggagtggaa ggcaaatttt atatctggaa aaaagaagaa atcgaaaatg
      601 tgttaggtga tggtacccag cttttttgta aaatatacaa cgtgaccaga tcaggtaact
      661 gggagggcca caatattctt aacctggctg attctgttaa agaatattgt gtcaaaaata
      721 agcttgatta tcatcagaca acaacggatc ttgaaaggca gaaaaaaata ttgctcaaca
      781 agcgaaatga aagagtcagg cctggcacag atgataaaat cctcttaggt tggaatgcac
      841 tcatgttgac cgcttatgct gaagccggta tggcacttgg caataagacc tacattacaa
      901 ttgctgtaga gcagcacaaa tatattaaag atatgttcag gcgtgatgat ggcgctttca
      961 atcatacata taaagatggc aaagcgaaga ttgatgccat gtttgatgac tacgcttttt
     1021 atattgaagc ccttattgct ttatttcatt caacacagga agtatcctat ctgtatgagg
     1081 caagagaatt gacggaatat atgtttggag aattttatga agaagtggac ggaatgtttt
     1141 acttcaccgg tgcacatgcc gacaacatcg tgcgcaagaa agatttcttt gatagtgcca
     1201 caccaagtgg aaatgccgtt cattgtcgca atctcatgta tcttggtgta atatttgatt
     1261 tgaaaaaata tagcgatacg gccgaaagaa tgctgaacgc tatggatgcg agtgtaagac
     1321 tttatcctac atcatttgca tactgggcta agaacaggat gatacatcat tatggtctgg
     1381 aggaagtagt cacttcagga aggtacaaag accaatttgt agaacaaatc aatatgatat
     1441 acagtgcggg taggatattg atgaagaccg ggatagggag aggcgatctt atattattgt
     1501 taaataaaga agaaactgaa gaaagcttaa tatatttgtg caaagactat aattgtcaga
     1561 gacccgttca tagtattgag gcatttgttg aaatgttgaa taaataagat gagaatttta
     1621 aaagtgtaca tattaatatt gtcaggaatg ttgcttttgc catcaattgc aaggtcgcaa
     1681 caatcaacac agtattcttt gtatcactta gacaagcacc tttttaaccc ggcctatgca
     1741 ggagtggagc ctgaacttac agcaactgct attgtcagaa accaatggct tggaataaat
     1801 gggagtccat tgtaccagca gttatcattg aatgcacctt taatagcagg aaactcgggt
     1861 ataggtctta attttgaaaa tgacaatctt ggtctggaac ataatataaa tgcatccgta
     1921 tcttattcgc accggtttcg gttttctgaa aatgggtttc tggcggcagg tataagagga
     1981 gtatggtcag gaaaatctct tgacggtaca aagataacaa caccagacgg aaaatacgaa
     2041 ccgggtagta gtccggacca taaagatggt ttattacctg taggaaagag cagggggaat
     2101 atatttggca taggtgcagg tattttgcta agagccggga atttcgaagt tggggccgga
     2161 gttgacaaca tagtgccggg cgaatacaaa ttcgaagcgt tccaaatcaa atacgatccg
     2221 acttggtatt tgaatgcagg gtggttatta ccggtatccg atttttggca attgaagctt
     2281 gacggtttgg ctcgtacaga tggtacactc gtacagactg atgcagttgt taaaattttg
     2341 atgggaaata atatgtttgc agggtcatcg ttcagaggat atagtaatcg tacgattgat
     2401 gcaatcagtg tatttgccgg aataagggta atggagagtc tgacactggc tgcatcgtat
     2461 gacttagggt tatccgatct aagagatata cacagtggta gtatcgaatt tgggctgaga
     2521 tatagctatg gcaacaaaat ttttagggaa aaactacctg tggtaattta taatccaaga
     2581 tattaaaatt ttctttaggg tagttaaaaa aatgtcatac agaataaaaa tttatttttt
     2641 ttaattttaa aaaattttat tttgatttta aaaaacataa atgttaaaat ttgagaaagt
     2701 taacgggggt agtggtaatt tttttttgca aaaacaatta atacaattat ctttacggtc
     2761 gctttactat attaactaat tagttggaaa aaaatcaggg ttgtaaacaa tgaaaaaaca
     2821 tttaagactt ttgccttttt taacgctttt gttgattctg tcgtcgtgtg gcagaaaaga
     2881 gaacgggcag ctaatcggag ttcttgacag gccaacttgg aagaacatca atccttatgg
     2941 catggtgtac ataccatccg gtacattcac acttggtcag agtgatgaag atgtaagacg
     3001 cacttacatg cagcgccaga aggctgtgtc tatccagggt ttttacatgg atgatacaga
     3061 aatcacaaac aacgaatatc gtcagtttgt aaactgggtg tcagattcaa tagctcacac
     3121 catgatggac cacatgactg aagatgatca gggtaatcag acaattgact gggagcagca
     3181 acttgattgg actgacgaaa cactcaatga tttatattat cagggagatg ataaatttgc
     3241 cggtaagaaa gagattaaca ccagcaaact ggtttatgct tatgagtggt acgattggca
     3301 agctgcagca cacgaccatg gcaagtcgcc tcgtaacaaa tttatcaaaa gagaaaagat
     3361 caagatttat ccagatacga tggtatggat aagagatttt gcttactctt ataatgagcc
     3421 attcacacgt aattatttct ggcatccggc atttgacgat tatccggtag taggtgttaa
     3481 ctggcacatg gccaatgcat tttgttattg gagaaccaaa ctctggaatt cttaccgtgc
     3541 ttccaagaaa ggcgaagtta actcagagga cttccgactt cctacagaat atgaatggga
     3601 atacgcagcc agaggtggcc gtgcagaagc accatatcct tggggtgcat attatacaag
     3661 aaatgcaaaa ggttgtttgc ttgccaactt caagccaggc agaggaaatt accctgaaga
     3721 tggtggtttg tacacagtaa aggcggatgc atacttcccg aatgactacg gactccgaaa
     3781 tatggcaggt aacgtagcag agtggaccat cacagcattc tcagaaaacg gtcagtcatt
     3841 cattcatgat cttaatcctg atatcagata tgatgctaaa gcggatgatc cgatagctaa
     3901 taagagaaaa gtaatcagag gtggatcttg gaaggatgta gcgcatttca tccagactag
     3961 tacacgtcat tacgagtttc aggatacaac caagtcttac atagggttcc gttgtgcact
     4021 tacattcttg ggaagatcta tcaatgattt ttaattttag ataagagatt aaaattttgt
     4081 cggcagggag ttataaaaat ttactctttc atacggcatt ttattatttg ctcatttgga
     4141 ataaacaata ttaattaacc aatttttaaa tcacatttgg tatgagtatg ggttttgtta
     4201 aaacagcagg ttttaagtat ttgaaaaatt ttattatcgg ggttggtgct gcgatagtat
     4261 tgataggtgc acttttcaag ctagagagtt ggccttatgc atctgaaatg cttatcactg
     4321 gtctatcagt agaagctttt atcttcttgt ttcttggtat tatcggtcct gagaaggatt
     4381 attactggga aaagctttat ccgggtcttg atgattatca ttcgcacatt tcatccatca
     4441 cacagggcga aaagccggct gcagctgttc cgcaattaaa cggtcaagtt gtagaaaaca
     4501 aacttggtgg aatgttggat gaactacagt caatgtcaaa aagccttggt tcactaaaag
     4561 cacttcagga agctgacttc agcggtacag ctgatcaggt gaaagctatg ggtaattttt
     4621 atgctaaatt gaatggtgca atgtcagaac ttgcacaatc agttgaagat acaaaagcat
     4681 ataaagataa ccttactgca ttgaacaaga acttaggttc attgaattca gtttatggtg
     4741 gaatgttgaa tgctatgtca aatattggca aaagctaaat caataccaac cagtatttaa
     4801 gacaaaagat ttaataatcg ttaaaaagac aataaactat gtcaatacct aaagagccca
     4861 ggcagctgat gattaacctc atgtatctgg tgctgacagc gctactggcg ttgaatgtct
     4921 ctgccgaagt aatgaatgct ttctttagtt tgaacaaagg tttggagaac agtaacaaaa
     4981 tcgtagataa tactaatatt acgattaaga atagtatcga caagcaagct gatgcaaaca
     5041 aaaaagaagt gaacatcaaa ttcaagaata gcgctgatgc aatgttaagt gaattgaaag
     5101 atttcactgc atacgtggat ggtataaaga aaacggtatt tgatgctgca ggaggtcctt
     5161 caaaggataa tcctactata cctgtaaata ttaaagacaa agatatccct acaagactat
     5221 tagtatatga taatgctaag aaaaaagacg gtatgggtgt cgagttggag aaaaaaatta
     5281 aagagttcag agataagctt atcaaatatg ctgatggcga ccagggaacc attaagtcaa
     5341 tctctttgga cactgaagaa gtgccaaaag gaagtgacaa gaagaactgg gctgatttca
     5401 gattccgcca gatgccggta gctgctgttt tccctatcct tactaagatt caatctgatg
     5461 ctaaaacatc ttctgctgca gtattgaact atatcaatgg taaagtagca ggtgaagact
     5521 taaagtttga taactttaaa gtagccattg cacctaaaac aggttatgtt gtaagaggtg
     5581 ataagtttga ggctgatgtt tatcttgcag catacagttc gaaccccggt caaggtgttt
     5641 ctatcagtgt gaatggtgct aacctcggaa tgaaggacgg tgttgctaaa tatacatcta
     5701 caccaggtag tatcggaaaa caaactgtgc gcgcaacagc aaccatcaaa aacccgctaa
     5761 ccggtgaggt gaaacgtgtt gatggtactt ttgactatga agtaggtgaa agatctgtag
     5821 cagtatctgc tgataagatg aacgttttct atattgctgt tgataatccg gtgtcagttt
     5881 ctgctgctgg tgtaagctct aataaactaa atgttggtgt tagtggtgaa ggtggtggtt
     5941 caatcactaa ggcaggttct accaattata ttgtaagagt atcacgaccg acaaaaccgg
     6001 gtgagttttg ctatatcaca gcaaatgctg acggactttc agacaaaaaa cctttccgtg
     6061 ttaagcgtat tccagacccg gttgcacgtt tgtcaaacaa agctaacgga gcgatgggta
     6121 caggtgagtt taaggcacag ggtggtttag gtgcattcct cgataatttt gactttgatg
     6181 caaaatgtgc tattgttggt tttacccttg taagagtacc taagagacag gatgctgtac
     6241 gtgaagccaa tgacggtgca cgttataatg gcaaatgtcg tggacttatc gatcaggcaa
     6301 aacctggtga tacttactat tttgaagacg ttaaggccaa atgcccaggt gacaatgccg
     6361 gacgtcctat taacccacta gtttttacta ttaaataaaa gaattctaaa atgaaattcc
     6421 agttgaaatt atttcttttt attgcttgtc tggggggggc ttctgtgtca tacgctcagg
     6481 tacctgaaac tatcaagaca gagtcaagtg aaccaggcga aactcctttg gatgacgtag
     6541 tagaaagaac cattaatgtc gaaaagagag tcttgaaata tgacaaccca cgcgaagttg
     6601 atttgatgtg ggaaaaaaga gtgtggagag taattgattt gcgcgagaaa atgaattttc
     6661 ctttcacata tcccgaagaa acttttgcag gtatcttgtt gaatggcgca aaagaaggta
     6721 aaattcgggt ctatagtaca aaggacgata aatttactga tcctctcaca gcagctaacc
     6781 ttgaatcaaa gttggtgaag attgatacta tcccgactgt tgaccctgag acttatgaag
     6841 aaaccatcca gatagttaaa aatgaaatta acgagaatga cttcaaaaga atccgcgtaa
     6901 aggaaatgtg gtattttgat tctgaaactt caacaatgaa ggttcgaatt cttggtttgg
     6961 ctgccattcg cgatgtgttt gatgataacg gtaattttaa atacgaagaa cctatgttct
     7021 ggatttatta tccacacatt agagaggagc ttgcaaagca ccgtgtattt atggaaggca
     7081 atgatgcatc tccattgaca tgggaggatt tcatggaaat gagatttttc tccagttatg
     7141 tttacaagga atcaaacgtt aaagataatc gtcttagcga ttacttgagt ggagttgatc
     7201 tgcttatgga aggtgagcgt attaaggccg atattttcaa ctgggaacac gatctttggc
     7261 aatattaatc ttgaattaat atgtacgata tattaaacgg tggcagaaat tgttctgcca
     7321 ccgttttctt tttattgtta actaagtctt ttatcacctt gtgtctaata catatattca
     7381 taagcagaat gatattgccc tgcatcgttt acatgatcga ttagcgttga atagaatttg
     7441 tctccgctga gtgtggcact atctccgatg atggccagat gtaactttgc tcttgtcatg
     7501 gcaacattca ttcttctata atctttcaaa aaacctattt ctccctttac attggatcgt
     7561 accagactga tgatgacgat atcactttcc tgcccttggt agccatctat actgctgact
     7621 cttatattta aattatacag agtttcatct tcctgtatcc gatcattgat gtaccttacc
     7681 tgtgctctgt acggagtaat gacgacaata tttttctcct tgagttcgag atgctccgat
     7741 attaggcgga gcagatgttc gcggagtatg aagtattcac cttcgttgta tttgctgaga
     7801 tacatgtctt tggatgtttc ttcaggcatt tgttcttcaa aaccggtacc agctgtatcg
     7861 atgaatatta cggctttgaa ggtctgattt tgagtttttg attgaatttc caaagcagtt
     7921 tgtaattttc ctttataaaa atacacgttt gaaaatgtca tgatatcatt gtgcattcgg
     7981 tattgagtat cgagaaggta agaaggataa ttcaaactta tactcttttc gagtaatgaa
     8041 atatcaagac ctgcttttgc cgccttagtc gatttcactg tcggaggaag ttgtagtggg
     8101 tcacccgcca taactattct gtcagctttt aacatcgtta tccaggctgc gggctccagt
     8161 gcctgcgatg cttcgtccaa aaaaattgtc gaatattttc tgcctgatag ttcatcattg
     8221 ttgcaagatg taagtgtcgt agctattacc tgtgccgaat cgagaatgct ttctgttatt
     8281 ctctcttcca gatcttttgc ccacgcttcc aggtcttttg cttcttttct tagccgggtt
     8341 cgttcttctt tttctgtatg tgtaaaatgt cttttatatt tggatgcttc actacgtaca
     8401 gcagctgctt caatcctgat ttttttgata tttggaaatt cacgatgtga ctctaccatt
     8461 ccgtcaagtg tgtgtgcgag taaagtttcg tcaatgcgtg agatgttccc aattcgtacg
     8521 gtttttactc ctgcctgcac aagtcgttcg accaatagat cgacagccac attgctgggt
     8581 gccgttacaa gtacttgctt ttctgtttct acggtctttt tgataatctg taccagcgtt
     8641 gtcgtttttc cggtgcccgg agggccgtga atgatggcca aatccttact ttgcatgacg
     8701 tgctgtaagg cttggttctg actttcattt aatattggaa tcgtgaacgg atatgtttca
     8761 tgcgaaaaga ttggtggctg cagacctgcg aatatttttt tgaattctcg tacacgtggt
     8821 tcttttgagt taatgacttc ttcgagtgcg ttctccatca ggcggtaggt acgttcgtcg
     8881 aactctgcct gcacggccag aggtggtttg ttgagccatt ccggcaattc acgtgcattg
     8941 gatatgagtt tcatggtatt tttgcctaca gaatatacgg tggccttcat ctgattgtct
     9001 tcatcatttg tgccttctga gtaaaatatt acaggtcgcc ctggttgcaa ctgatggttt
     9061 ggtgatgtga cagatttgtg ctcaaccgtt atagaaatac gaccacccat cgtaaaaccc
     9121 gttttggtaa ctaccaccgg acgccatagg tagcccgcct caattttttg ttttacggtg
     9181 agcgtagcta gctgattatt atgaaaagta aaatcttctt ccttctcgag tttgagtaat
     9241 ctctgtagtt ctatataatg tttgtttatt tcttcgagtt ccacaagttc tgtattttat
     9301 gaaatatatt ggcttcgtat aacagcatgc ccattacgat cagatattct gctataacta
     9361 tccatagatt ttctgcaaag gtatccgcat tgtagttgaa atatgtaaaa aatatcagcc
     9421 cgctccagac aaaaggaaat ctgtaacggg taaatatgcc tgtgaataac attaaactca
     9481 agtaccaagg atgcacagta gttgaaaaaa taatataaat gaatattagc caaaacattc
     9541 tatacaacaa ttctttattg tccgattttt tataggaata cagtatgatg attgagaaag
     9601 aaatagcact cagcagtgga ccgatgaatc gtatctgatt gtagccacgc agtttgaaac
     9661 ctgcccacct tagccaatag tatattccgg cattgaattc aaaattcctg aaatataggt
     9721 cgagactgtt gaagatgctc gtgttttgta taacaggtag gcttagcatt gctgtgaaaa
     9781 ttcctagtag aaggatgaat tttaatttcc tgcggcgaat ttcaccaaaa aacaacagag
     9841 ggaaaaatat agctgtaagc attttggaag ctaccgataa tgcaaatgtc agtgcagaaa
     9901 ggaatttatt tccattcaaa tagtagaata taccggctgc aagaaaagct gtcatggctc
     9961 cttcaaaatg tgcattacca catatttcta taactatcag tggatttaga gcataccaaa
    10021 agattttctc aggtgctatg cttagctttt tgagcaatct gacagaaaag tacattgtgg
    10081 cacattcgca gaaaaacaaa aaagccttga tgagaatagt gttgagcatt atgttgtcac
    10141 ctgcgaggta actgcttaca taaaataccg actggcacac caatggataa acgctgaaat
    10201 attccggtga attgagcctg ttgaacaatt gttgatccac tacagtaagt ttaaccgact
    10261 ccatcagccg ggcaggtgta gcctcaaaag ggttaatgcc ctgaacgagc aacctaccgt
    10321 cccagatgaa gcggtaaata tcgtcagaca acttcggaaa ggcaaaaatg agcacaaacc
    10381 tgactgcaat tgctgaaagc aaaacggtat tgagccgaag tgtgcttttt acggctaggt
    10441 accagtagca accaaatgat agcatccaaa gactgaacaa ctgaatatgc tggtgccttt
    10501 ctactgcaaa accagcataa atgatggtgg ccgaccaaag tgcgagcaat atcacttcac
    10561 gggcatcatt gcctgaatgg cttagtgcct ttatttctgg acgtgcttcc atgaaagtgt
    10621 agctatagag aaaaatccta cgaacaacaa tacttgtaca ccaagaaaac tgatgttgcc
    10681 gtacaaaata cctgctgtgc ctgcaattac aaaatacaaa gccataaagt attccatccg
    10741 ggtagagaag gacattttat ttgatctgta ggttgtgtat tttaacttat cagttttacc
    10801 gacaatatta tactttggtg tacgaataaa ggctgatttc ctgccacgaa atccgtcaag
    10861 aacagcaatg gcattgtgta atgacatgcc aagtgacaaa gacagcaaca acggaaacaa
    10921 aactacaaat accaagacat ctttaaagct aaaggttttc ctgacagccg gattaaaatt
    10981 gcctacaaga tagaccggaa aaatcaaagc ccaacccacc atgaatacgg atgcccattg
    11041 tagtttatat cctgtttggt ccagcaggaa aatggccgga aacgaaagta atgccgtaag
    11101 aaaaaccagc aaaaacactg aacttgacaa cagataagct gtggtaaaca tcttttttgt
    11161 aaacgggagg tcagacttga agacagcggg taatagtttt cttgcacatt ccgcaccgcc
    11221 tttcatccat ctgtactgtt gcgatttgaa gccgttcatt tctacgggca gttcggacgg
    11281 tgtgcctgtt tcttcgatat atttaatttt ccagcctttt agttgtgtcc ggatactcaa
    11341 gtcgaggtct tcagtaagtg tgtcggcact ccagccacct gcatcctcga tggcctttct
    11401 gcgccagatg cctcctgtac cattaaactg aagataaagg ccggcataca tcctggctgt
    11461 ctgttctatc gagaaatgta cgttaagtgg taatgcctga agcctggtga ttaggctgta
    11521 atcacgattg atgtattccc agcgggtctg cacaacagca tacctttcat cctgaaagaa
    11581 gtaaggaata catttcaata taaagtcttc atcgggcaga aaatcggcat cgaaaatggc
    11641 tatgaacgct ccttttgctg tagtaagacc gtttttgagt gcacctgcct tgtagccggt
    11701 acggtctgta cggtgtatca gttcaatgtc gattccccgg gtgcggtaat attccacttt
    11761 tgccgatgag tatgtgagcg tttcatctgt agaatcatct aatatctgta tttgtaaaag
    11821 tctggcaggg tagtttatct ttacaatatt gtctattagg cgttctacaa catactgctc
    11881 attgaacacc ggcagctgga tggtgacaaa tggtaaatcc gcctcagtta aggtgttcat
    11941 tccttttgta taaggatttt tgaagcgata aattaaatac agaaccataa aatatgcctg
    12001 aatgaggcaa tagactgtta ttaggaaaag gcatgttaca taaatataaa gaaatatgat
    12061 ggctgctact tgcatgttct tagttgttta gccagagttt cccgattgtc cacaatattt
    12121 tatatccggc acttatgctg ccctttatgg tgccagacac cttgcttgtg cctatacgtt
    12181 tacggtaatt aaccgctatt tctgttgtct ttagtttgtg tttggcagct tttacctgca
    12241 tttccactgt ccagccaaaa tttcggtcag ccatcttgag tgcaagtagt gaaggatagc
    12301 gaattgcccg atagggcccc aaatcagtaa aacgatatcc gtacaaccac ctgatgagtg
    12361 tggtcgaaag ccagttgccg aatacctgtg gtgtggtcat cgagcctctt tctcgtctgc
    12421 caagtgctct tgacccgatc accatttcca tgccatcatc caagattggt tttacgagct
    12481 catacatttc ttcaccatag tctgaataat cgccgtccat aaagacaagg atgtccggtt
    12541 tttcatcaga cggcagtgct tcgatgtacg ccataccccg caggcaggca ttgccatacc
    12601 cgggcgatgg ttcgtctgct acaatcgctc ctgcagtaga ggcaaccacg gctgtagcat
    12661 cactgctgtt gttattgcac acgatgatgt ttcgtaccag atctttcgga atgtccctga
    12721 tgaccttagc tatggaaagc tcttcgttat atgccggtat gatgacgtca ataatcgctc
    12781 ttgccataga tacaaaagta attctttttt acttagcaat atgtatgttt taatagtagg
    12841 ttacagcgca tatcgctcac caactgcaag cggcctgaaa ttttgtgaga aactgcctct
    12901 tgatttcgat tcggccacca ttcgcaatgg ttcacccagg ggttcgtccg ataagtcgta
    12961 ggtgccgtag tgcatcggta tgaaattctt gccgttcagc attttaaagg caaggatggc
    13021 ttctttcggg ttgaggtgta ggcctcgcat cataaatgcc ggtttgtaac tgcctattgg
    13081 tatgatacaa tggtctatct gctttgtcag aaagcgtatt ttgtcgaaat gctgcccgaa
    13141 tgccgtatct ccggcgaaaa aaatactcgt tttgtcagat tctataagga agctccccca
    13201 caattgttga tcaagatcaa acatatatct tcggttccag tgttgggcag gcagataaac
    13261 tatgcgcaat gatccagtat tgctgaatat ctgataccag cctgcttctt cggtctgagc
    13321 atgccacgct ttaggcaaga gtccggaagt gccaagtccg gcaagtatcc tgactcccgg
    13381 attattcttt accagtatct tgagagttgg caggtcgagg tggtcgcggt gtgcatgcga
    13441 cagcagaatg aagttaagcc tggttatttc ttcagttttg aaaggcagtc cgacttttct
    13501 ttttttgaat ggtagatttc gcaaacaagg atcggtgatg atggtagtac cttgtagtcg
    13561 gatgaggaac gtcgcatgcc caagccacaa tagttgattg tccgtttgat ggaagaactc
    13621 attgcccttt ataagcggaa gacggtattc gtccctgcgt ttttcacttc gttgttcatt
    13681 gaaagaaaac atccacttga agaaagtctt ccaagaaggc atcaccctcg attcgtcgct
    13741 tatgtaacgg cctttgtaat aagtgtttcc cggatattct ggtaatatcg tagccagagc
    13801 agggttgttt ttcaactgat agtgcttcac agtagttgta ttctggtgca aagattttcc
    13861 tttttttaat taattcatac tttatcggat gatagttttg tattatcggc ccgattcttt
    13921 ctaaaatgca atgatggatt ctttgcctat accaatgtgc ggttcgatct gtctgttttc
    13981 agaatgcaaa aaaggccatc cgctaacgga cagccttcaa aaattgagca aaaaacagtt
    14041 cttataatgt atcttgtggt tcagccgtat cttcgtgctg tccttcagat tcttcggtat
    14101 tttctttagg gctcgtgttt acaaatccgg ccttgcccat tctccatatt tttccggaat
    14161 attcatagct cgattcctgt tcaggatctt tttctatctt atagacaaag ttctcagttc
    14221 ttgatgttac cttcagatat tttccttcag gtacatatac attcactctt acctcctggt
    14281 ctcgccatac ttttccttga atgatctcga agtagcccgg gaatataagc ctcgagccat
    14341 cttgaccgat agggtacagg atagagttgg cgagcgactc tgcttcctgt gtgctaccgc
    14401 cattcgactc aaggtttact ttccagtaaa tgttctggtc gtccgatttc agcactcgta
    14461 tatgtatgtt gtcggagtaa aacttcccgt tcttggtacg gagctctcct aactgaatgt
    14521 tgtcaaaatc gcccgaatca aaagtttcat tttcgttgat gtctatcacc agtgtgtcag
    14581 gcattgtagc tgcaaagtat tcattcgctt tgcttacctc agcatggctc ttgaatgacc
    14641 gggcaaaatt gacactcgaa aatatcccaa gcaaaaaaaa cagcatccat gcaaagccaa
    14701 gacctttgag caaacccgaa ggcgtcttta cctgaaagaa agtcttcaga cctaatacta
    14761 tcagcgctac tgcaggtatc agaaacacca gaatcaacga tgtgacaaac agccaatgct
    14821 tgctgaacat tcccggtccg aacatcaggc tcgaataatc atgcagaaaa aatcccgaag
    14881 taagcagtgc tatccccgcc gttgctatgg caaacataca tatcaaccca atgagtatga
    14941 taaaaccttt tattatctta gggatgatgt actgcagtgc ctctaccagt tcaccaacaa
    15001 aattggtgat acccgagaac gcatacggtt catttgcgtt cagattattt ttttttttgc
    15061 cactgaactc ccgacctatg tcgttgattc tttgcccgaa gtggttgaag ctatcctcaa
    15121 cctcctttgc tatattgttg atgttgatcg gttcgccctt cattgacagg cggtcagctg
    15181 atgtcacagc cttaggtatc agtatccaca atatcatata aaacaagaag cccgtgcctg
    15241 ctacaaagaa caacaacaag aaaatgattc ttaaccagat cgggtcattg ataccgaagt
    15301 acgacgacag accagcacac acaccaccga gtatcttctc gtcagggtcg cggaatatcc
    15361 tcctgccggt ctttatacca ccactgcgtt ggccagtatt aaagttggct tgagtgcctg
    15421 cagtgtccac accaaaatcc tcaagcgagc ccatcgtgtg tatcgcatag tcaacatctg
    15481 cagtcgaaat gatgctcctg cccctcagat tgtccgcaaa caactcaccc atacggctct
    15541 ctatgtcgtt ggttatctcc tcgtagcctt ccgagttctt gaagtaatcg tgtatcgaag
    15601 tcaggtaata gtcgagtttg gcataagcat cttcatcaat gacgaatgct cttcctccca
    15661 gattgatgtt tatcgtttta ttcatgattt ggtcttgttt tgaatggttt ggttaacaga
    15721 ttgaacgagt gacgcccatg attctttcag cgagtcataa aattcgcttc ctttaacggt
    15781 cagtgtatag tacttgcgtg gtggccccga tgacgattcc tgccatgagt agtccagata
    15841 ccccgagttc ttcaaccggg tgagcaatgg gtacaaggtg ccttccacta ctatcaactc
    15901 actctccttg agacggttga tgatgtcaga agggtaggcc gcttctcttg acaatatagc
    15961 cagtatcacg agttccagta ctcctttccg catttgtgat tgtgcatttt ccaaggcttt
    16021 tgtttttagt taatgaataa ttgatgatgc aaagataatt catcgccaag tattatgcaa
    16081 tacatagaac taattatatt taagtacatg gcataaaatg gatgtatgta atggatgata
    16141 tcgacgaatg tacgtttttc atcgacgaaa ttgccggatt tgtttccctg aatataatcg
    16201 aataatgaat gtgcaggcgt attttttcat ctatctgttt tcatttgggt gtgcccctgt
    16261 cgggtcgggc tatccgcctt actttgtgcc ggcttct
//
LOCUS       DAOQQY010000031        16117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1134_length_16117_cov_6.209501, whole genome shotgun sequence.
ACCESSION   DAOQQY010000031 DAOQQY010000000
VERSION     DAOQQY010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16117
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1134_length_16117_cov_6.209501"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="PLE23_03115"
     CDS             complement(<1..1623)
                     /locus_tag="PLE23_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82287.1"
                     /translation="MFINTRVGLIGRIFCIVLLATPALYAQSVKEQKHDAIRKLDSGH
                     YTEALSILQNLYSNGPHDPDISTALAKAYLYTNQTAESLDLIDRTLALDKKNQAELYY
                     LKACNLHLRHNFSEAIRWYKSALSFKKSEKNAAAIKDQIIRCATGLKLSKVQGNYIID
                     NLGNTVNTIHDEFAPILSPNYSTKLYFASANDKSNGGQRNDQGIADVYGSYSSDIYES
                     FLINGTWTNTNPISYMINGPRNEYITGFSGRGKEMYFFRGFTLYSGDILVDTFQKNID
                     QQMNPQYYRGPVNAAKGDRDLTFVNDTLICFSSNRPGGYGGFDIYFSKLVKGRWTEAV
                     NAGDAINSAYDEVTPFLKDDATVIYFSSNCLNSIGGFDIFSSTYDAVAKKWSLPTNAG
                     IPFNSAADDLYFRLAPGEYYGYFASSRLESYGKRDIFSVYFPDIIINSIRENVAEVTE
                     TDIQKSEANLKTNQNSNTSVQTLYGHEKKEIEQDFEVQSMFWDDATKLLTPKNVQYLN
                     SVTTILKQNKSVRLILTGHVAGTSDAKADAYIS"
     gene            complement(1623..2960)
                     /locus_tag="PLE23_03120"
     CDS             complement(1623..2960)
                     /locus_tag="PLE23_03120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HMX82288.1"
                     /translation="MAKFELVMPKMGESIMEATILKWVKKVGDTVDIDETVLEIATDK
                     VDSEVPSSAKGVITEILYPENTTVEIGKVIAIISQEQAGDAASVPAAAAEIKSTATAP
                     AAQATQTTVPGATQSEQIPTTSGNKFYSPLVKNIAEKESISINELNSIEGSGTNGRVT
                     KKDILSYVEAKSNTTQTTAPQVTSQPATTQQPSPTTTETPVNKPAQSYGGNVEIIEMD
                     RMRKLIADHMVMSKQTSPHVTSYVEIDVTPIVQWRDKVKGPFEKKYGEKITFTPIFIQ
                     AVARAIADFPMINISVDGTKLIRKKDINIGMAAALPSGNLIVPVIKNADMLNLVGLTK
                     SVNDLANRARANQLKPDEIQNGTFTLTNVGTFGNVMGTPIINQPQVAIMAVGAIRKKP
                     AVVETEYGDVIAIRQMMFLSLSYDHRVVDGSLGGSFLRRVGDYLERFDTNQEI"
     gene            3164..4108
                     /locus_tag="PLE23_03125"
     CDS             3164..4108
                     /locus_tag="PLE23_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMX82289.1"
                     /translation="MLNNHLYTAFYSLYYSHTDMPTNKNISKPTTLHWVTLWSLGLLW
                     GCSFFLIKKGLVCFTPVQTASIRIGVTSLAFLPFFIWHFKKINWKNLKYYAIVGLAGS
                     GIPAFLFANAQTEVSSSVSGILNSLTPLFTLIIGIVFFNNKENVRKFFGVLLGLGGAA
                     ILILYGKELNTGGHISYSLLIILATICYATSVNTVKKFLQEENPLVLSAVAFQIIGIP
                     SLIVLFNTDFRGKLQLDHEAWVALGYLVILAFVGTFMATVLFFGLVQKTDALFGSMTT
                     YIIPIMAIFLGLLDGEVLEISHGMGMAAILGGVYLSRK"
     gene            4105..4491
                     /locus_tag="PLE23_03130"
     CDS             4105..4491
                     /locus_tag="PLE23_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HMX82290.1"
                     /translation="MIQHIPSLYIGNSAFKGRGVYTSAEIQAGDLIEICPVIVCNEED
                     LPLIHKTRLHDYYFLWGEEEEKCAIALGYGSLYNHSYNPNARYEVDFDEKVIRFYALS
                     TIKAGGEITVNYNGDWQDQNPVWFDK"
     gene            4622..5893
                     /locus_tag="PLE23_03135"
     CDS             4622..5893
                     /locus_tag="PLE23_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMX82291.1"
                     /translation="MEKHLVASHMAENMSGSEIIKLAWEINARISEGQHIYNLTIGDF
                     NPNIFPIPQYLTDKIKEAYNNHQTNYPPGEGIPALRDAISHYLKRESEMQYSTNEILV
                     AGGSRPLIYAIYKTLLDPKDTVLYPVPSWNNNHYAHLTGCKAIEIETLPENNFLPVLD
                     DIKKYLSKVNLLALCSPLNPTGTCFSKKTLNAICKAVIKENEKRRIKGKKALYIMYDS
                     IYWGLASKKAEHFDPVSLHPELRDYTISIHGISKVLAGTGVRVGWAWGPEKIIRKMKA
                     LLGHIGAWAPKAEQVATAQFLDNHDEVKSYLAWINKEVSYRLETFYDGFQALNDKGYQ
                     VDALRPQGAIYLTVLFDLIGRKTKSGKTLETIADVSTFLIDEAKVALVPFSAFGSKTK
                     APWYRLSVGTCTKEDVGKILDSLEAALASLR"
     gene            complement(5910..6881)
                     /locus_tag="PLE23_03140"
     CDS             complement(5910..6881)
                     /locus_tag="PLE23_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX82292.1"
                     /translation="MTDFKFSSDVEGIDVLAHSYKELAKEIGKVIVGQDEVVRSVIIS
                     LFSNGHSLLVGVPGLAKTLLVSTIADVLDLDFKRIQFTPDLMPSDITGSEILDESRHF
                     KFTKGPLFANIVLADEINRTPPKTQAALLEAMQERSVTVSGQRHLLPNPFFVLATQNP
                     IEQEGTYPLPEAQLDRFMFNITLDYPSYQEELEVVRTTTSSHKAELKHVVNTQQILAF
                     QNLVRRIPIAENVLEYAVKLATKTRPGTEMATPMVNNYISWGAGPRASQYLVLGAKCH
                     GALRGKYSPDIEDVQAIAKSVLRHRIVRNYKAEAEGINEQQVIESLF"
     gene            complement(7246..10611)
                     /locus_tag="PLE23_03145"
     CDS             complement(7246..10611)
                     /locus_tag="PLE23_03145"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMX82293.1"
                     /translation="MKDVFKEFKPTSWSIDNRKSIYFITLLLAIAGIYSYNTLPKERF
                     PDIVIPTIYVNTVYPGASPADIENLVTKPIEKEIKAISGLKKLTSQSVQDYSIVMAEF
                     NTDVDVSDAKQKVKDAVDKAKSELPDDILEDPSVLEVNFSDMPIMYVNISGDYDLNKL
                     KKYADDLQDRIESMSEITRVDMVGALDREIQINVDQYKMDAAGVTLAEIERSIQYENM
                     TISGGQITTDEMKRSLSVTGEFKDLSQFDNIIIKSTAGSIVYLRDIAKIVDGFKEQES
                     YARLDGKNVITLNVVKRSGENLIHASDKIRDIIKELQSSTFPKNLKITITGDQSDNTR
                     ITLHDLINTIIIGFILVTVVLMFFMGVTNAFFVAMSVPLSICVAFLVMPSLGVSLNMI
                     VLFAFLLALGIVVDDAIVVIENTHRIFHNGNMSIVQAAKYAAGEVFMPVFSGTMTTLA
                     PFIPLLLWKGIIGKFMIYLPITLVITLLASLVVAYIINPVFAVDFMKKDEHAEDVKVN
                     RNWTSIIIVAVIAIVFYVFGSFGMGNLVTLFAILMILYKLFLKRVILHFQQDVWPRAQ
                     NAYADLVSWAIHRRRPVYLMLGLVLLFIFSIIFTAARGPKVVFFPQADPNFVYVYLSM
                     PVGTSAEHTDKTVKSLETKVMGVLGEKNPLVESVITNVAIGATDPAENDRTTASNKAK
                     ISISFVKFAERKGKSTQDILDNIRANVKGIPGAVVSVEKESNGPPTGQPISIEVSGDD
                     LSMLNETSIGLKKYLDSMQIAGVEELKSNLQNSKPQITVNIDRERANMLGISTAQVGA
                     DLRNAIFGKEVSKFRDENDEYPIMVRFDEVQREDLSSLINTKVTYRDMAMGGMLRMVP
                     LSSFATIEYSNTYGAIKRKDQKRIVTIGSNVLSGYNPNEVVQDVMTAIKSYPKPDGIN
                     IRMGGELEEQAETSAFLGMSLMISLGLILLILVTQFNSISKPLIILSEVIFSIIGVLL
                     GFSIFKMDFSILMVGVGIIALAGIVVRNGILLVEFTDLLREQGMPLKEALVEAGRARM
                     TPVLLTATATILGLIPLAIGFNVDFVTMFTELNPKIYFGGDSVAFWGPLSWTMIFGLI
                     FATFLTLVMVPVMYLLDEKISERFSKVFRRIKK"
     gene            complement(10641..11780)
                     /locus_tag="PLE23_03150"
     CDS             complement(10641..11780)
                     /locus_tag="PLE23_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMX82294.1"
                     /translation="MKYAYYILALLLVSCGGGTSTQAPKSLDALKKKLTAKEDQVLQL
                     NKEIAELQDQIAKLDTTRSFKKILVSTKSIEPTKFTHSIDVQGQITGDELVNVSPQVP
                     AVYAKIFVTKGSKVTKGQILATLDDKVLRQGIAELKSSISFAKTIYDKQKALWDQKIG
                     SEIEYLSAKNTLESLNNKLATMNQQAVMYKLRSPINGVVDDVFPKEGEMGSPGMPSIR
                     VVNLNRLKVVAHLAEAHIDRVKPGNKVEVTFTDLNKTIHSTVKTVGKVISELNRTFTV
                     DIALPSSPDYRPNMLCIVKILDYQNAAAMTIPINLLVKSENETYVYSVQDKNGVLRAV
                     KTPVQIGLISDGNVEIKSGLKTGDRVITVGYQDINEGDAVEVSQQ"
     gene            complement(11800..13140)
                     /locus_tag="PLE23_03155"
     CDS             complement(11800..13140)
                     /locus_tag="PLE23_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMX82295.1"
                     /translation="MKHYLTLVALMFSTVWVFGQQAGQFTLQQAIDYAMSNNTEVKIS
                     QNNIRDAEKQITERTAAGLPQINGTVSYNYYAVIPTTILPEQFAIDPTTGQVNPNFDR
                     AVKFGVKNNLTGTASLSTLLFDGSYLVALQAAREYRDYAHEELKSKQYTVKNQVTMAY
                     LPVLLINLSLETLDKNIANLQNLSKEVTAIYNQGLGEQLDVDRINLSLSNLTTEKDNL
                     LRQKDIAINYLKFVMNYPVDKQIEIVDNISSIESKPADGILSGKISYDSRPEYGVIKK
                     GIRLNELNVKVNKAGYLPSLVAIGSFQYNIQDDKFFSKDAIASPTAIIGGQLNIPIFD
                     SFAKKAKTERARIQMLNALEQKNMFERSVDLEVTNARQSYNAASERLEMQRKNVALAE
                     KIYNTTKTKYQHGLGSSLEITQAEQSLYSVQQNFNQALYDVIVAKINLDKAFGK"
     gene            complement(13137..13745)
                     /locus_tag="PLE23_03160"
     CDS             complement(13137..13745)
                     /locus_tag="PLE23_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMX82296.1"
                     /translation="MELKQRIIKESRCLFSRFGIKCISMDDISQKLGISKKTLYQEVT
                     DKNELVNEVVADILNSERVELEEIFKSSEGAIDEMIKITQHVIQLLSSINPATLLDLQ
                     RHHPALYDKIKKYQYDFIYKVIRNNLEKGMTEGIYRDDLNADVVAKLYLTKAMTIADE
                     TVFPTDKYNKTTIFVEHVIYHLKGVISEKGTKFLNQYIQTIQ"
     gene            complement(13887..14255)
                     /locus_tag="PLE23_03165"
     CDS             complement(13887..14255)
                     /locus_tag="PLE23_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82297.1"
                     /translation="MKYLVILLLVFSGYYANAQAQSRFVIDDDATIKVMLTNNVTDAE
                     LKELRALLHDKRGFLFTWSNVVREKAKVKSMTISIDCNDGRFRSSTTVDDFTDNKSVG
                     FYRHYSDSLPHPFWIGIEEK"
     gene            14423..14728
                     /locus_tag="PLE23_03170"
     CDS             14423..14728
                     /locus_tag="PLE23_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMX82298.1"
                     /translation="MKKLLFSILMIALVIACNTQNNKDSDMPKTGQEIYRRNCALCHG
                     ADGKLGVSGAKDLSTSPMGLDERINRIAYGKDLMTPFTGLLTDEEIRLVAEYTLTLK"
     gene            14730..15860
                     /locus_tag="PLE23_03175"
     CDS             14730..15860
                     /locus_tag="PLE23_03175"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HMX82299.1"
                     /translation="MDTNNITTYKIIGLMSGSSLDGLDVVHCLFDIVHIPEFKILNWQ
                     ITHAETITFDDEILKKLNEAVDYSGRNLVQFDHELGKVFGNMTANFIKRNNLSPDFIS
                     SHGHTVFHATQLGFTLQIGHPAEIVATTELPVVGDFRSTDIALKGQGAPFAPIVDHWL
                     FSDYDILVNLGGIMNITFLNTPDGVIAYDIGPCNQTLNYLASLTGAEYDKDGTMAAAG
                     KTDDNLLQKLKAWPYFELPIPKSLDNSHIKTNFHNIIDNHTATVQNKLATMTELIAFK
                     LAETVNSNKYDSIPRIMLAGGGAFNNYLVSRMKHYSGDAEMILPDKSVISYKEGLLLA
                     LMGLLRIHNIPNVLKSVTGSKTDHIAGAVYQGIKKQIHEQYR"
     gene            15844..>16117
                     /locus_tag="PLE23_03180"
     CDS             15844..>16117
                     /locus_tag="PLE23_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMX82300.1"
                     /translation="MSSTDKNVKILVTGASGYLGSNILRHLHTAGYQNLTGLSRSLPD
                     ADEFDFRLNHIPVDILDVLGLEDAFAGHDIVVHAAGLVSYQPADKQK"
BASE COUNT         4610 a         3401 c         3086 g         5020 t
ORIGIN      
        1 ggagatataa gcatcagcct tagcatctga ggtacctgca acatgacccg tcagtatcag
       61 tctaaccgat ttattttgtt taaggattgt agtaacgctg ttgagatact gaacattttt
      121 tggtgttagt aattttgtag catcatccca gaacattgat tgcacttcaa aatcctgctc
      181 gatttctttt ttttcatgcc cgtataaggt ttgcaccgat gtatttgagt tttggttagt
      241 cttcaaattt gcttccgact tttgaatgtc tgtttcagtc acttccgcca cattttctct
      301 tatgctatta ataataatgt ccggaaaata tactgaaaag atatcgcgtt tgccgtacga
      361 ctcgagccga gatgatgcaa aatatccata atactctccg ggtgcgaggc gaaaatacaa
      421 gtcatcagct gcagagttga atggtattcc ggcgttggtg ggcaaagacc atttttttgc
      481 tacagcatca taagtactag aaaatatgtc aaagccacca atactgttca ggcagttgct
      541 actaaaatat attactgtag catcgtcttt taaaaaagga gtaacttcat cataagcaga
      601 attgattgcg tcaccggcat tgactgcttc tgtccagcgc ccttttacca actttgaaaa
      661 ataaatatca aacccaccgt aaccgcctgg cctgttactt gaaaaacaaa ttagcgtatc
      721 atttacaaat gtcagatcgc ggtcgccctt tgctgcgtta acgggtcctc ggtagtactg
      781 agggttcatt tgctggtcta tatttttctg aaaagtatca accaaaatat ctccgctata
      841 caaggtaaac cctctaaaaa aatacatttc tttgcctcga cctgaaaacc cggtaatata
      901 ttcattacgc ggaccattta tcatgtacga aatggggttt gtgttggtcc aggtaccatt
      961 tatcaggaag ctttcgtaaa tgtcgctgct gtagcttccg tacacatctg caataccctg
     1021 gtcattcctc tggcctccgt tacttttatc atttgccgat gcaaaatata attttgttga
     1081 ataattgggg cttagtatcg gtgcaaactc atcatgaatc gtattcacag tattgccaag
     1141 attatcgata atataatttc cctgcacttt tgaaagctta agtccggtgg cacatcttat
     1201 aatctgatct ttgatagcag ctgcattttt ttcagatttt ttaaatgaca aggcagattt
     1261 ataccatctg atggcttcag aaaaattgtg cctcaggtga agattacaag ccttcaggta
     1321 gtatagttcg gcttgatttt tcttgtccaa agcaagtgta cggtctatta ggtctagtga
     1381 ttcggctgtt tgatttgtat agaggtaggc tttggcaagt gctgtactta tatcaggatc
     1441 atgcggacca ttgctgtata gattctgaag aattgataaa gcctctgtgt agtgtcctga
     1501 atcaagctta cgtatggcat catgcttctg ttctttgaca gactgtgcat acaatgccgg
     1561 tgttgccagt aaaacaatac agaaaatccg gcctattaac ccgacccgag tattaatgaa
     1621 cactatattt cctgattggt atcgaatctc tccaggtaat cacctactct tctcaggaat
     1681 gaaccgccaa gtgacccgtc aactactctg tggtcgtagg aaagtgacaa aaacatcatc
     1741 tgtctgatgg caatgacatc tccgtattct gtttctacta ctgccggttt tttcctgata
     1801 gcgccaacag ccataatcgc cacttgtggt tgattgataa ttggtgtacc cattacatta
     1861 ccaaaggtac ctacattggt gagtgtgaat gttccgttct gtatttcatc aggtttcagc
     1921 tggttggctc ttgctctgtt agccagatcg ttaacacttt tggtaagccc taccagattg
     1981 agcatatcag cattcttgat aaccggtaca ataagattgc ctgaaggaag ggcagctgcc
     2041 atacctatgt tgatatcctt tttacggatg agctttgtac catctaccga aatgtttatc
     2101 atcggaaaat cagctattgc cctggcaact gcttgtataa atattggagt aaaggtaatt
     2161 ttttcgccat acttcttttc aaaaggccct ttgactttat ccctccattg tacgataggt
     2221 gttacatcta tctctacata agatgttacg tgcggtgatg tttgttttga cataaccatg
     2281 tgatcggcta tgagtttgcg cattctgtcc atctctatga tttctacatt gcctccatag
     2341 ctttgtgcag gcttgttaac cggagtctct gtagtggtag gtgatggctg ttgtgtagtg
     2401 gcaggttgag aagttacctg tggtgcggta gtctgtgttg tatttgattt tgcctcaaca
     2461 taactgagta tatctttttt agtaacacgt ccgttggtac cactaccttc tattgagttg
     2521 agctcattga tggatataga ttccttttct gcaatgttct ttacaagtgg tgaataaaat
     2581 ttattgccgg aagtagtagg tatctgctca gattgtgtag ctccaggcac agtagtctgt
     2641 gttgcctggg cagcaggggc agtagcagtt gatttgattt cagctgcagc agccggcaca
     2701 gatgctgcat ctccggcttg ttcttgagaa atgatggcaa tgaccttacc tatctccacg
     2761 gttgtatttt caggatataa tatttctgta ataacgcctt tcgcagagct aggcacttcg
     2821 gaatcaacct tgtcagtagc tatttcgaga acagtttcat caatatctac ggtatcgccc
     2881 acttttttca cccacttcag gatggttgct tccataatac tttcgcccat tttaggcatt
     2941 actagttcaa attttgccat gacagttttg ttttaatttg aaatgcaaaa atataagaaa
     3001 atattgaaaa atcagacctc ttgcacgaat attctcccaa gacgtgtaca gagttctata
     3061 caaagcaaaa attatttttt ttgagcattc aaatttaaat atttaacatg ataaaacatc
     3121 cgttgaaatc attacactta aaacaaaaca ccataagaaa aagatgttga acaatcatct
     3181 ttatacagcg ttttactctc tttactactc acacacagat atgccgacca acaaaaacat
     3241 aagcaaacca actacactcc actgggtcac attatggagt ctcggattgc tttgggggtg
     3301 ttcattcttt cttatcaaaa aaggtctggt gtgttttacg cctgtacaga ccgccagcat
     3361 acgtataggt gttacttctc tagcttttct tccgtttttt atctggcatt ttaagaaaat
     3421 caactggaaa aacctgaagt actatgccat tgtggggctg gctggcagtg gtattcctgc
     3481 atttttgttc gccaatgcac agaccgaagt atcgagttcg gtatcaggta ttctcaattc
     3541 acttacacca ctttttaccc ttattatcgg tatagtattt ttcaacaata aggaaaacgt
     3601 caggaaattt tttggtgtat tgttaggttt aggaggtgct gccatattga tcctttacgg
     3661 taaagagctg aatacaggtg gtcacatttc ttacagcctc ctcatcattc ttgctaccat
     3721 ttgttatgct accagtgtca atactgtaaa aaaatttctg caggaggaaa acccgcttgt
     3781 acttagtgca gttgctttcc agatcatagg catcccgtca ctgattgtac tgtttaatac
     3841 agattttagg ggcaagctcc aacttgacca tgaggcatgg gtggctttgg gttacttggt
     3901 cattttagct tttgtaggta catttatggc tactgttttg ttttttgggc ttgtacaaaa
     3961 gacagatgcg ttattcgggt caatgactac gtatattatc cccatcatgg ctatatttct
     4021 tggactactc gatggcgaag ttttagaaat ttctcacggt atgggtatgg ctgcaatatt
     4081 aggtggtgta tatttgtcaa gaaaatgata cagcacatac cttctctata catcggtaac
     4141 agcgcgttca aaggcagagg cgtttataca tctgcagaga tacaagctgg tgacctcata
     4201 gagatttgtc cggtgatagt atgcaatgaa gaagatttgc cactcataca caagacacgg
     4261 ttgcacgatt attatttctt atggggcgaa gaagaagaaa aatgtgccat tgcacttggc
     4321 tatggttcat tgtacaacca cagctataac ccaaatgcaa ggtatgaagt ggattttgat
     4381 gaaaaggtca tcaggtttta tgcattgtcc acaataaagg caggaggaga aataactgtt
     4441 aactataacg gagactggca agaccaaaac cctgtatggt ttgataaatg aaaaaactac
     4501 actctggttg tattaaataa aaagctttga tatttttctt ggtttatttc aaaatttaac
     4561 aaaacctttg tacctttcca ttcttagctg tacaaacaat tcaactttca aaccacagtt
     4621 tatggaaaag cacctcgtag caagccacat ggccgaaaac atgtcaggat ctgaaatcat
     4681 caaacttgca tgggaaatca acgcacgtat cagtgaaggg caacatattt acaatctcac
     4741 cataggtgat ttcaacccaa atatcttccc catcccccaa tatttaacgg acaaaataaa
     4801 agaagcatat aataatcacc aaactaacta tccgccgggt gaaggcatac ctgccctgag
     4861 agatgccata agccattacc taaagcgtga gagcgaaatg caatacagta ccaacgaaat
     4921 actcgtagcc ggtgggtcaa gaccactcat ctatgccatc tataaaacac tcctagaccc
     4981 taaggatacc gttctctacc cggtaccaag ctggaacaac aaccattatg ctcaccttac
     5041 aggttgtaag gcaatagaaa tagaaacatt gcctgagaat aatttcctac cggtattgga
     5101 cgacatcaaa aaatacctgt caaaagtcaa tctgctggct ctgtgctctc cattgaatcc
     5161 tacaggtact tgcttcagca aaaaaacact taacgctatc tgtaaggctg tcatcaagga
     5221 gaatgaaaaa cgcaggatca aaggaaagaa agcgctttat atcatgtatg attctattta
     5281 ttgggggctt gcatctaaaa aagccgaaca ctttgatccg gtaagtctcc atcccgaact
     5341 acgcgattat accatcagca tccatggtat atcaaaggta ctggcaggca caggtgtaag
     5401 agtaggatgg gcttggggcc cggaaaaaat aatccgaaaa atgaaagcac tactcgggca
     5461 cataggtgct tgggctccaa aagccgaaca ggtggctaca gcacagtttc tcgataatca
     5521 tgatgaggta aaatcatact tagcctggat aaataaggaa gtaagctaca gactggagac
     5581 cttctatgat ggatttcagg cacttaatga caaaggctat caggttgatg ccctcagacc
     5641 gcagggagca atctatctga cggtgttgtt tgacttaatc ggacgtaaga caaaatcggg
     5701 taaaacactc gagacaatag cggatgtaag cacattcctc attgatgaag ctaaggtagc
     5761 actcgttcca ttcagtgcat ttggctctaa aactaaagcg ccgtggtatc gtctgtcggt
     5821 aggtacatgt accaaagaag atgtaggcaa aattttagac tcactggaag ctgcacttgc
     5881 aagcctacgc taaatgttgg cttggcgtac taaaataggc tttctattac ttgctgctcg
     5941 ttgatgcctt ctgcttctgc tttatagttt cgtactattc tatggcgtag tacactcttg
     6001 gctatcgcct gtacatcctc tatatccgga gaatatttgc ctctcaatgc tccgtggcat
     6061 tttgctccta atacaagata ctgcgacgct ctcgggccgg ctccccaaga gatgtagttg
     6121 ttaaccatcg gtgtggccat ctccgtcccc ggccgagtct tggtagcgag tttgacagca
     6181 tattccagga cattctctgc tataggtatg cgccttacaa gattctgaaa cgcaagaatc
     6241 tgctgtgtat tcactacgtg cttcaactct gccttgtggc ttgaagtagt ggtacgtacc
     6301 acttcgagtt cttcctgata gcttgggtag tcaagggtga tattaaacat aaaacggtcg
     6361 agctgtgcct ccggcaatgg ataagtacct tcttgttcta tcgggttttg tgtagcaagt
     6421 acaaagaacg gattaggtaa taaatgacgt tgacccgaaa cagttacaga tcgttcctgc
     6481 atggcttcaa gcagggcagc ctgagtcttg ggaggagtac ggtttatctc atctgccaaa
     6541 acaatatttg caaacaatgg ccctttggta aatttgaaat gccggctttc atccagaatc
     6601 tccgaaccgg tgatatctga aggcataagg tccggtgtaa actgaatcct cttaaaatca
     6661 agatccagaa catctgcaat agtactcacc aacaaggtct ttgcaagacc cggtacacct
     6721 acgagtaagc tatggccatt gctaaataat gaaatgataa cagatcttac tacttcgtcc
     6781 tggcctacta ttaccttgcc aatctctttt gcaagctctt tgtatgagtg tgccagtaca
     6841 tcaatacctt ctacatctga tgaaaactta aaatccgtca ttttttgtgt tgatttatta
     6901 acatgaagta aacgcttttt ggtgttttat gttgcgtaac tgttcttttg gtagattata
     6961 ctccttacca taaaatctga atactcataa caaaataccc tgttgttttt ctctggataa
     7021 aattaaaaaa cctctgaact gatgacgtac agaggttaac aaaaaacaaa cactatgaac
     7081 aaaaacaatt tagaataagt tgtatggttt tataaccagc attgaacgta attgttcaaa
     7141 acaaaagcat ttctttattt ttattatact tcattattca aaccttataa cgcattcatt
     7201 tattatccta cattctgaaa agtaatcttt caccaacatt ctacattact tctttattct
     7261 tctgaatact ttactaaacc gttcagatat tttttcatct aaaagataca ttaccggtac
     7321 catcaccagc gttaggaatg tagcgaatat cagcccgaat atcatagtcc aactcagtgg
     7381 accccagaac gccacactgt ctccaccaaa atatatcttg gggtttagct cggtaaacat
     7441 tgtaacaaag tcaacattaa acccaatagc caatggtata agacccaaaa tagtagcagt
     7501 ggctgtcagt aacaccggcg tcattcgtgc acgtcctgct tctacgagtg cttccttcag
     7561 aggcatacct tgctctctga gcaaatcggt aaactccact agcaaaatgc cgttacgtac
     7621 tactattccc gccagagcaa tgatacctac acctaccatc aggatagaaa aatccatctt
     7681 aaagattgaa aagccaagta gtacaccaat gatagagaaa attacctcac tcaggataat
     7741 cagtggtttt gaaatgctgt tgaactgtgt caccaggatt agcaatatca agccaagcga
     7801 tatcatcaga gacataccca agaacgctga tgtttcggct tgttcttcaa gttctccacc
     7861 cattctgata tttatgccgt caggttttgg gtaagatttt atagccgtca ttacatcctg
     7921 cactacttcg ttaggattat agcccgaaag tacatttgag ccaatggtca ctatcctttt
     7981 ctggtcctta cgcttgatgg caccataagt attgctatac tcaatagtag caaaggatga
     8041 caaaggtacc atacgaagca tgccccccat agccatatca cggtatgtta ctttggtgtt
     8101 gataagggag gacaaatctt cacgctgcac ttcatcaaac cttaccatga tcgggtactc
     8161 atcattttca tccctgaatt tggatacttc cttcccgaag attgcatttc tgaggtctgc
     8221 acctacctgt gcagtagaga tacccaacat atttgcacgc tcgcggtcga tgttaacagt
     8281 tatctgaggc ttactgtttt gcaggtttga tttcaattct tcaacaccgg ctatctgcat
     8341 cgagtcaagg tatttcttga gcccgatgga ggtttcattc agcattgaca ggtcatcgcc
     8401 tgatacttct atactgatag gctgcccggt aggtgggccg tttgattctt tctcaacact
     8461 gactaccgca cccggtattc ctttcacatt tgctcttatg ttgtctaata tatcctgtgt
     8521 tgattttcct ttacgctccg caaattttac aaaagaaatt gaaatttttg ccttgtttga
     8581 ggcagtcgtt ctgtcgttct ctgccgggtc tgtggcacct atagccacgt tggttattac
     8641 cgactctaca agtggatttt tttcaccaag tacacccatc actttagtct cgagcgattt
     8701 tactgtcttg tctgtgtgtt cagccgaagt accaacaggc atgcttaaat atacataaac
     8761 aaagttgggg tctgcttgcg gaaaaaacac gaccttaggc ccgcgtgcag ctgtaaagat
     8821 gatagaaaat ataaacaaca acacaagtcc gagcatcaga tagacaggcc ttcgacgatg
     8881 aatagcccat gatactaaat ctgcataagc attttgtgct cgtggccaaa catcttgttg
     8941 aaaatgaaga atgaccctct tgagaaataa tttatacaat atcatcaata tggcaaacaa
     9001 ggtaacaagg ttgcccatac cgaagctccc gaatacataa aacacgattg ctatgactgc
     9061 cacaataatg atagaagtcc agtttctgtt tacttttaca tcttctgcat gctcatcttt
     9121 cttcataaaa tctaccgcaa agacgggatt aataatgtaa gcaacgacca gcgatgctag
     9181 caaggtaata actagtgtta tgggcagata aatcataaat ttaccaataa tgcccttcca
     9241 taacaacaaa ggtatgaatg gagcgagcgt agtcattgta ccagaaaaaa ccggcataaa
     9301 cacttcgcca gcagcatatt ttgccgcctg tacaatactc atgttaccat tgtgaaatat
     9361 ccggtgcgta ttttcgatga ccactatagc atcatctact actataccaa gtgccagtag
     9421 gaaagcaaac aatacaatca tattgagtga cacacccaaa ctcggcataa caaggaaggc
     9481 tacacaaatt gataatggca ccgacatggc tacaaaaaat gcgttcgtga cacccatgaa
     9541 gaacatgagc accactgtta ccaaaataaa ccctatgatg atggtattga taaggtcgtg
     9601 aagcgtaatc cttgtattgt ccgattgatc gcctgtaatg gttattttca gatttttcgg
     9661 aaaagttgaa gactgcaact ctttgatgat gtcccgtatt ttatccgatg catgtatgag
     9721 gttctctccc gatcgtttta caacattgag cgtaattaca tttttaccat caagcctggc
     9781 atacgattcc tgttctttga aaccatctac tatcttggca atgtctcgca gatacacaat
     9841 tgaacccgca gtactcttta tgataatatt gtcaaactgg ctcaaatcct tgaattctcc
     9901 cgtaacactt aatgatcgct tcatttcatc tgtagttatc tgaccaccgg aaatggtcat
     9961 attttcatat tgtatggagc gttctatctc agcaagggtt acaccggcag catccatctt
    10021 gtactgatct acattgatct gtatctcacg gtcaagagca cctaccatgt caactctggt
    10081 tatctccgac attgactcta ttcgatcttg aaggtcatca gcatattttt tcaacttatt
    10141 caggtcataa tcgcctgaaa tattgacgta cataataggc atgtccgaaa aattcacttc
    10201 aagcacactc ggatcttcta gtatgtcatc cggcaattcg gattttgcct tatctacagc
    10261 atcttttacc ttttgcttgg catccgatac gtccacgtct gtattaaatt cggccattac
    10321 aatagaataa tcctgtaccg actggcttgt gagtttcttc aatcccgaaa tagcctttat
    10381 ttccttctca atcggcttgg ttaccagatt ttcgatgtca gccggtgatg cacccggata
    10441 tacagtattt acatatatgg taggtatgac gatgtcagga aatcgttcct tcggaagggt
    10501 attatatgaa tatattccgg caatagccaa caacaaggta ataaaataaa tactcttgcg
    10561 gttatctatt gaccaactcg tcggtttaaa ttctttaaac acgtctttca tcttaaaatt
    10621 tttgatttta ataaatctaa ttactgctga gatacttcca ctgcatcacc ttcattaata
    10681 tcctggtagc ccactgttat cactctgtca cctgttttta aaccactttt tatctcaaca
    10741 ttaccatctg atatcaagcc tatctgcaca ggagtcttca ccgctctcag cactccgttc
    10801 ttgtcttgta ctgaataaac ataggtttca ttttcagatt ttacaagcag attgatagga
    10861 atggtcatgg cagcagcatt ttggtaatca agtattttga caatacaaag catgtttggc
    10921 cgataatcag gtgagcttgg taaggcaata tctactgtaa atgttctatt gagttcggat
    10981 attacctttc ctactgtctt cacggtcgag tgaatggttt tgttgaggtc agtaaaggtc
    11041 acctctactt tattgccggg ttttactcta tctatatgag cttcggcaag gtgcgctacc
    11101 actttgagac ggttgaggtt tacgactctg atggagggca tacccggtga gcccatctca
    11161 ccttctttcg gaaaaacatc atccactact ccgttgattg gtgagcgaag tttgtacatg
    11221 acggcctgct ggttcatggt tgccagttta ttattcaaag attcgagtgt attttttgct
    11281 gacagatatt ctatttcaga acctattttc tggtcccaaa gagccttttg cttatcataa
    11341 atagtctttg caaacgagat actactcttt agctccgcaa ttccctgtct gagcactttg
    11401 tcatcaaggg ttgccaatat ttgtcctttg gtcactttcg acccctttgt gacaaatatt
    11461 ttggcataaa cggcaggcac ctgcggactt acattaacga gctcatcacc ggttatttga
    11521 ccttgtacat caatggaatg tgtaaatttg gttggttcta tactcttagt agataccagt
    11581 atctttttga atgatctggt tgtatcaagc tttgctatct gatcttgcag ctcagcgatt
    11641 tctttgttca gttggagtac ttggtcttct ttggcggtaa gttttttctt aagtgcatcc
    11701 aggctttttg gtgcctgggt acttgtgcca ccaccacaag aaacaagtaa aagtgcaagg
    11761 atataatatg cgtatttcat tttctttctt ttaattaaat tatttaccaa atgctttatc
    11821 aagattgatt tttgcaacaa tgacgtcata caacgcctga ttaaaatttt gctgtaccga
    11881 atacaagctt tgttctgcct gggtaatttc gaggcttgag ccaaggccgt gctggtattt
    11941 tgtcttggtt gtgttgtaga ttttttctgc cagagcaaca ttcttgcgct gcatctcaag
    12001 cctttcgctg gctgcattat aggattgtct tgcattcgtc acctccaggt caacactacg
    12061 ttcaaacata tttttttgtt ccagcgcatt cagcatttgt attctggcac gttctgtttt
    12121 ggctttcttt gcgaaactat cgaaaatagg tatattcaac tgcccgccta ttatagctgt
    12181 aggtgacgcg atagcatcct tggagaaaaa cttgtcatcc tgaatattgt attgaaatga
    12241 accgatggct acaagtgaag gcagatatcc ggccttattg accttaacat ttaattcatt
    12301 aaggcgaatg ccttttttga tgacaccata ttcgggtcgt gaatcatagc ttatctttcc
    12361 gcttagaatg ccatcagctg gttttgattc gattgagcta atgttatcca ctatttctat
    12421 ttgcttatca accggataat tcattacaaa tttgagataa ttgatagcaa tatccttttg
    12481 tctgagcaaa ttatcctttt cggtggtcag atttgataaa gacagattga ttctatctac
    12541 atcaagttgt tcgcccagac cttgattgta tatggccgtt acttcttttg acagattctg
    12601 aagattggca atgtttttat ctaatgtctc aagactaagg ttgatcaaca aaaccggcag
    12661 ataagccatc gttacctgat tcttaaccgt atattgtttt gatttcaatt cctcgtgtgc
    12721 ataatcgcgg tactccctgg cagcctgcaa tgctaccaaa taactgccat caaaaagtag
    12781 tgtggaaagc gaagccgtac ctgtcaggtt gttcttcaca ccaaatttca cagcacggtc
    12841 aaaattcgga ttgacctgcc cggtagtagg atcaatggcg aactgttccg gcaagatagt
    12901 ggtaggtatc acagcatagt aattgtaact gacagtaccg ttgatctgag gtaaaccagc
    12961 tgcagtccgt tctgtaattt gtttttcagc atctctgatg ttgttctgac tgattttaac
    13021 ctcagtgtta ttggacatgg catagtcaat agcctgctgt agtgtgaatt gcccggcttg
    13081 ttgtccaaaa acccatacag ttgaaaacat caatgctaca agtgtcaaat agtgtttcat
    13141 tgtattgttt gaatatattg gttaagaaat tttgttcctt tttcagaaat tactcccttg
    13201 aggtggtaga ttacgtgttc tacaaatatg gtcgttttgt tgtacttgtc cgtcgggaaa
    13261 acggtctcat cggctatagt cattgccttt gtgagataca atttagccac tacgtctgca
    13321 ttaaggtcgt cacgataaat accttctgtc atccctttct caagattgtt tcttattact
    13381 ttgtatataa aatcgtactg atattttttg attttgtcat acagcgcagg atggtgtctt
    13441 tgaaggtcaa gaagcgtagc cggattaata ctacttagca gttgtatcac atgttgggta
    13501 atttttatca tttcatcaat ggctccttca gaagatttaa atatttcttc cagctccact
    13561 ctttctgaat tcaggatgtc ggctactact tcatttacga gctcgttttt gtccgtaact
    13621 tcctgataca gtgtcttttt cgaaattccc agcttctggg aaatatcatc catcgaaata
    13681 catttaatac caaatctgct gaacaaacat cttgattcct taataatcct ttgttttaac
    13741 tccattatat atttgaaata ttttgcaaaa ataatcaatt aaactacgga aactaaaaaa
    13801 ggtttaaaag ttttttaata aaaataaaaa aactgccccc cggattggga gcagttatca
    13861 tctggttatt tatgttatga ataaaactat ttttcctcta taccgatcca gaaaggatgt
    13921 ggcagactat cactataatg cctataaaat cctaccgatt tgttgtccgt aaaatcgtca
    13981 actgttgtac tcgacctgaa acgaccgtca ttgcagtcga tgcttatggt cattgatttt
    14041 accttagctt tttcgcgcac cacatttgac caagtgaata agaagcctct tttatcatga
    14101 agcaatgctc ttaattcttt aagctctgca tccgtaacat tattggtaag catcacttta
    14161 atggtagcat catcatcaat aacaaatctg ctctgcgcct gagcatttgc ataatatccg
    14221 gaaaaaacca gcaacaaaat aactaaatac ttcattttca ttctaattta aaggctaaag
    14281 ataattcatt ttttttaaaa atacaaatat ttaaccttca gatgatttgt aaagtatttt
    14341 tgttgcctga aatcacaaaa tacaatctta tttaaccaaa gagtcacgta cctttgccaa
    14401 tccatctacc aaggtattct ttatgaaaaa acttttattc tccatcctca tgattgcact
    14461 ggttattgcc tgcaatacac agaataataa ggatagcgat atgccaaaaa ccggccagga
    14521 aatataccga cgcaattgtg ccttatgtca tggtgccgat ggtaagcttg gtgtaagtgg
    14581 agcgaaagac ctcagtacat cgcctatggg tcttgatgag agaattaacc gcattgctta
    14641 cggaaaagac ttgatgacac ctttcaccgg tctgctaacg gatgaagaaa taaggctggt
    14701 agcagaatac actctaacac tcaaataaat tggacacaaa caatatcacg acctacaaaa
    14761 taataggact catgtcgggc agctcgctcg acggactcga tgtagtgcac tgtttgtttg
    14821 acatcgtaca tattcctgag ttcaaaatac tcaattggca aataacccat gccgaaacca
    14881 ttacattcga tgatgaaatc ttaaaaaagc tcaatgaagc tgttgactac tcaggacgta
    14941 atcttgtaca atttgatcat gaactaggca aggtgtttgg aaacatgacg gcaaatttta
    15001 ttaaaaggaa taatctttca cctgacttta ttagttctca tggccatact gtattccatg
    15061 ctactcagtt aggctttacg ctacagatag ggcatcctgc tgagatagta gccactaccg
    15121 aactacccgt agtgggcgac ttcaggagta ccgacatagc actcaaaggt cagggtgcac
    15181 catttgcgcc catagtggat cactggttgt ttagtgatta tgacattctg gtcaatctgg
    15241 gtggcattat gaatattact tttcttaata ccccagatgg tgtaattgcc tatgatattg
    15301 gaccatgcaa ccaaacactt aattatctgg catcgctgac aggtgctgag tatgacaaag
    15361 atggtacaat ggcagctgcc ggaaaaacgg acgataattt gcttcaaaaa cttaaagcct
    15421 ggccttactt cgagcttcct attcctaaat cgctggacaa ctcacacata aaaactaact
    15481 ttcacaatat tattgacaat cataccgcaa ctgttcaaaa taagcttgcc accatgaccg
    15541 aacttattgc cttcaaactt gctgagaccg tcaatagcaa taaatacgat tctataccca
    15601 gaataatgct tgcaggtggt ggggcattta ataactatct cgtcagcaga atgaagcact
    15661 attcgggtga tgcagaaatg attcttcctg acaaaagtgt tatttcatac aaagaaggct
    15721 tacttttggc acttatgggt ctattgcgga tacacaatat acctaacgta ctaaagagtg
    15781 ttaccggcag caaaaccgac catatagcag gagctgttta tcagggcata aaaaagcaga
    15841 tacatgagca gtacagataa aaatgtcaaa atacttgtca ccggtgcatc cgggtatctg
    15901 ggtagcaaca tcttgcgaca tttgcatact gccggctatc aaaacctgac aggactctcc
    15961 agatcactac cagatgcaga cgaattcgat tttcggctta accacatacc agtggatata
    16021 ctagatgtac ttggtttgga agatgcattc gctggtcatg atatcgtagt acacgctgcc
    16081 ggattggtat cttaccaacc cgccgacaaa caaaaat
//
LOCUS       DAOQQY010000032        16013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1145_length_16013_cov_6.715566, whole genome shotgun sequence.
ACCESSION   DAOQQY010000032 DAOQQY010000000
VERSION     DAOQQY010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 16013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16013
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1145_length_16013_cov_6.715566"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="PLE23_03185"
     CDS             complement(<1..137)
                     /locus_tag="PLE23_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HMX82301.1"
                     /translation="MKTGLFFGSFNPVHTGHLIIANFMATHTDLKEVWLIVSPQNPLK
                     D"
     gene            complement(141..560)
                     /locus_tag="PLE23_03190"
     CDS             complement(141..560)
                     /locus_tag="PLE23_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82302.1"
                     /translation="MKIGSFIFVLSMCCLTLFACKKDESPETALNNWLYLIDNGKFEE
                     AKKLSTENGQYLVNVISKISEEPDTSRHLFKSLSCRVIKDSAVCKFAYEEGIYDSVYL
                     KKVNEKWLFGGNFEELDEGLDEFFDEMIEENSDSSVY"
     gene            complement(604..1722)
                     /gene="dnaN"
                     /locus_tag="PLE23_03195"
     CDS             complement(604..1722)
                     /gene="dnaN"
                     /locus_tag="PLE23_03195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMX82303.1"
                     /translation="MKFSTSSSQLLKHLQIASGAITNNPVLPILEDFLFTIKDNRLKI
                     TASDLDTTIITHIDITAEKDGSIAIPAKILTETLKALPEQPVSFEVDESSNHVTLTSA
                     YGTYKLSGENGVDFPETPESPTDEEIVIPTDILASAITNTLFATSNDDLRPTMTGVYF
                     LFEEGKATFVATDAHKLVKYSRSIDNEAVSSSFIMPKKALNLIKSSLSNTDEVRISFN
                     KSNAFFTFGHFQMACRLIEGRYPDYNMVIPVNNPNLMTIHRTDFLNSLKRIAIYANKT
                     TNQVLIQIDGEQLHISSQDLDFSNEAQETITCTYDGVPIQIAFNAKFLIEMLTILSST
                     DVTLELSEPNRAGVIRPAEQDPSSDLLMLVMPVMVNVQ"
     gene            1856..3130
                     /locus_tag="PLE23_03200"
     CDS             1856..3130
                     /locus_tag="PLE23_03200"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMX82304.1"
                     /translation="MDRKIAPPVKDIRKVILPRPEICTLSNGITIFGTQRPGLPVLRL
                     EIVVMAGRPFERKKIISKLTGKLLKEGTTSKSSRKIADIFDKYGATIHTSANLDYVTI
                     SVHCLRKYFFQLWPVVYEILREPAFPEAELNTYVANNIQKLEIDLTKPEVVAYRLITE
                     KIFGNDHPYGYNSTPELYRQATRADITEHYQRAFIPSKTNIHISGGYEAEDIDLIAES
                     MGAWQNPHLSEPVFAGLISDKPGNIHIEMPTSSQASLRIGRRMFSRSHPDFIDVFILN
                     TLLGGFFGSRLNMNIREGKGMTYSIGSSLDPLSYSGCLIIMADVSHEHIGKATRQVFK
                     EFKKLREIPIVNDELTQLKRYLIGSLINAVDGPFHSSAMIKYLIADQVDLNIWDKANE
                     RICDITTEEIQHTAQQYLKQEDYWVVSAGINT"
     gene            3290..4321
                     /locus_tag="PLE23_03205"
     CDS             3290..4321
                     /locus_tag="PLE23_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMX82305.1"
                     /translation="MKFFNFFTQELAIDLGTANTLIIQDGKIVINEPSIVAIDRKTGE
                     TIAVGTKAMQMHEKTHDSIKTIRPLKDGVIADFQAAENMIEGFIKMIGVRKKFFTHMK
                     MVICIPSGITEVEKRAVFDSADHVDSKETYLIHEPMAAALGIGLDVEEPVGNMVIDIG
                     GGTTEIAVIALSGIVCDQSIRTAGDEFTMDIIDFMKREYSLLVGERTAEQIKINVGAA
                     IEDLANPPADYAVNGRDMVSGIPKQIMVGYQEIARALDKSIAKIELAILKALETTPPE
                     LASDIYQKGIYLTGGGALLRGLDKRISDRTKLKVHIADDPLAAVVAGTGIALKNTHRF
                     SFLIDRKSV"
     gene            4353..5177
                     /gene="mreC"
                     /locus_tag="PLE23_03210"
     CDS             4353..5177
                     /gene="mreC"
                     /locus_tag="PLE23_03210"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HMX82306.1"
                     /translation="MLNVIQFIIRFGTFFLFLGLEIVCIYLIVNYNKAQQDIFLNSAN
                     LLSGSVNEKFTKVRSFMNQYSVVDSISQENAALYQERFNYPFLFDTLDNFKDTSLVYS
                     VIPARVINNSVSTQHNYFTINKGTSDGVQKGLGVITKNGVAGIVKSTSKHFALVISIL
                     NDDLKTSASLTRNGYFGSLVWDGKDPKYFDLQDLPRHAETKVGDTIQTSGYSLIFPKG
                     ILLGTVKEIKQKEGTNFYSLTCRIWEDMSKLDIVYVLKNNAKPELEQLEKDVYTDK"
     gene            5158..5688
                     /locus_tag="PLE23_03215"
     CDS             5158..5688
                     /locus_tag="PLE23_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82307.1"
                     /translation="MSIQINKLHFSLLRIFLIIAIQVLVIKNIRLPWDKEGYGLFLFY
                     PVAIMFLPSQLSKPITLIIAFCAGITVDVFYDTPGVGAGALVAMAYARPLAFEVFEVR
                     LGIKLGQESHNLNPGFFTLLMYAAVLLLIFCFCYFSFEYFTIYYIEDIIIKTIYSFLL
                     SLTIVALYIVIFRPKI"
     gene            5695..7647
                     /gene="mrdA"
                     /locus_tag="PLE23_03220"
     CDS             5695..7647
                     /gene="mrdA"
                     /locus_tag="PLE23_03220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMX82308.1"
                     /translation="MKELFQIRQYYVMGIIALCGLIIVIKLAQIQIFDNSYSNRANAV
                     TIARQYIYPSRGLIYDRNNKLLINNDPVYELSVVVSEMDPAMDTVKLCNLLQVSKQEL
                     DEKLNIDWRDHRFSKSLPVVLFRDISPATFAALQEVLFEFPGFSVQQRNRRTYPFPNA
                     ANILGYIAEANEKQIDSSNGKYQPGDEVGVTGLEKYFEEELRGRKGVRYEMKNNVGKS
                     IGAYKEGKFDIVASAGKDIISSIDIDLQAFAEKLLHNKTGSIVAIQPSTGEILAMASS
                     PSYDPNLLVMGKDRGKAFQQLSQDTLKPFFDRAIMAKYPPGSIFKTLVGLISLEEGLT
                     TANRYIPCNGAYYYGAIKWGCHHHIHATSLPIALQVSCNTYFFTLTREIIDQYSFHLP
                     GKGLNTFAEHLHDFGLGAPIGIDYPTEKGGNVPSAAYYDKLYKRRRGWRSPTIMSIGI
                     GQGEIQLTTLQMANLAAIIANKGSYYVPHLVKGYADGTTKIEERFRTKHSVKIRPEHF
                     LPIIEGMERVVWYGTGRRAAIPGINFAGKTGTSQNPHGEDHSVFFGFAPAEKPEIAIA
                     VIIENAGKGAEFAAPIASLVAEKYLRKEISRPALAESMEAINTIRIKPKRKRLPYRTS
                     VAIDSLQSPPLAPSEEPIQAQNEEKQ"
     gene            7644..8138
                     /locus_tag="PLE23_03225"
     CDS             7644..8138
                     /locus_tag="PLE23_03225"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMX82309.1"
                     /translation="MIISTIVAVSENGVIGKNNQLPWHLPADLKYFKKITNGHHVLMG
                     RKCFESIGKPLPGRTNLVLTRNNNYQPDGIVKIHTIDDGINIAKEHGETELFILGGAD
                     VFRLTMPVIDKLYFNTIHAEFEGDTFWPKTDWTQWKLDSTEYHDADTNNLFPYSFSVF
                     TRRK"
     gene            8135..9400
                     /locus_tag="PLE23_03230"
     CDS             8135..9400
                     /locus_tag="PLE23_03230"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HMX82310.1"
                     /translation="MKEDLLQYFWRTHNWKDRLYVTTNGLPIHIVTPGILNHDAGPDF
                     SNAKIRIGDELWVGNVEIHVRSSEWYIHRHQYDPAYRTVILHVVWTEDQPVFMDENIR
                     IPCLALKSIISKEILDNYERLMNNALWVPCQNVVNRFDMEKGLLGMNARLVEKFEDKS
                     AVLQHIFDKTKGDWNETLHRFIARGFGLNVNGDAFELLAEKMNYQLTQKYQHDPVKLE
                     AIAFSASGLIPDGNSDHTEKLWQIYQTLPHNDRPTPLAQGVFKFFRTRPSNFPGLRIA
                     QWLKFLSNPGSLFDTVINLNSPIEFSAIICQPLDEFWKNHFSFTAEAKNFSTQAGKDF
                     ADLLLINSIAPFLFFYGKITDNYELRQKAVDLLDAMKAESNSVIRGFHQLGFPINFAS
                     HSQAFLLLKKKYCDRKRCLECPFGVQIFK"
     gene            complement(9406..10353)
                     /gene="rsgA"
                     /locus_tag="PLE23_03235"
     CDS             complement(9406..10353)
                     /gene="rsgA"
                     /locus_tag="PLE23_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMX82311.1"
                     /translation="MLNGVVIKSTGSWYQVRLEDNREWKCRITGKHKLFGWQLTNPVA
                     VGDYVQVEPEISAGQGVIKRILPRKNYIVRQSPRQKHQLHLLAANVDQACLITTIIQP
                     NLKQGFIDRFLLMTEPYNIPVIIVFNKSDLFGPVETEIYEELKALYTSLSYIVLKTST
                     VSKENFEELKSLLNNKTTLVSGQSGVGKSSLIMGIEPTLTLSTDTISDHSGKGQHTTT
                     FAEMHQTSFNANIIDTPGIKTLSFNHLEPTDIAHNFREIFEVSTACRYADCKHINEPG
                     CAVKQAVESGTIHHLRYQNYLQLLDEINDQNYWERHKDY"
     gene            complement(10346..11122)
                     /locus_tag="PLE23_03240"
     CDS             complement(10346..11122)
                     /locus_tag="PLE23_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HMX82312.1"
                     /translation="MSEAILQQLIISKKNGNKELGILLDPDKTDISQLPALIQLLEKA
                     AVDYILVGGSLLTTHRFDELVQILKKLTNIPLILFPSGTYQVSPHAHALLFLSLISGR
                     NPELLIGKHVEAAPLLHKLNLEIIPTGYMLIDGGIQTTVSYISNTTPIPYHKPEIGAF
                     TALAGQYLGMKLIYMDAGSGAIRPIPIEMIQTVISTIELPLIVGGGIKTPEQAFVTAS
                     AGADLIIVGNHFEKDPTMIPDFAAAIKSAGKSVKKPDFNA"
     gene            11224..12174
                     /locus_tag="PLE23_03245"
     CDS             11224..12174
                     /locus_tag="PLE23_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMX82313.1"
                     /translation="MKKILITDLVHELLIEGFEKAGYTVDYFPDIRYDEVCRIISNYF
                     GLIVNSKILVDKEFIDKAVRLTFVGRLGSGMEIIDRQYAEHRGIGVFNSPEGNCNAVA
                     EHALGMLLALANNLVCADREVREFVWKREKNRGFEIDGKSIGIIGYGYTGMAFAKKLL
                     SWDVELLVHDKYKTHIDTQSGRIKVVEKAELLKYADIISLHLPLTSETVSFVDEKFIE
                     QWEKKGVLINTSRGKVVKTTALLSALDRGELQGVCLDVFENEKPETYSADEKIFYENL
                     FRHKNSIFSPHIAGWTIESKEKLSKTLLNKILFFIESNNL"
     gene            12364..15990
                     /locus_tag="PLE23_03250"
     CDS             12364..15990
                     /locus_tag="PLE23_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX82314.1"
                     /translation="MIRRLLLFAILWVVGTGVYAQTSLEGKITEEDTGAPAELATIKL
                     FKGGVLVTGAQTDFDGFYSISPIDPGTYTIEASYVGFTTKQIEGVVVKQGRANIVNIK
                     LSKGVVINEVEIVAYKVPLIEQDNTTQGAVVTAEQIKNLPQRNVNAIAASTAGVSTSG
                     EGGSISIRGSRSANTVYYIDGIPVSGSFVPVWDLEQLQVITGGLSAEYGDVTGGVISG
                     ATKGPSQKFTGGAEVETSAPFDKYGYNLANLNISGPILKRKNSNESILGYRFSGSYTG
                     YKDSNPSAVGIYTAKQSVIDKIAADPITRINENFLAPSGEFLTSKNGDVDLVKVRPNE
                     ESKRYDLTGKLDWKITKKIDVTFSGTYSKDYSRFTPSSAWYLLNTQNNPESFSNISRF
                     DFRFRHRLGNSNYANDEEAKAQLSAFRNASYTLQFFYQNANSLEYDSRFKDRFFDYGH
                     IAEFDFGYRGALAFDQDFIPRHVDNTRYFIKYDPSNSKNPGLANYNKVIKDATSDLDY
                     IQRNGRANDSYTSIWGFHTNVNDIYNRYAKSRSERRTFQGTLGFDFVPGGSERGKHNI
                     QIGVFYEERISRSYAVAPRGLWTVAQLLQNQHIAGVDTSLVAEANHTFILPDGSQIVA
                     DSFANKIDDLDGRFYKEVRKTLGIGLDHYVEVDQIDPSLLRLDMFSSRELTNRGIASF
                     YGYDYLGNELSSSTTFNDFFTSKDANGKQLFPVAPAKPIYASGYIQDKFTFKDIIFRI
                     GVRADMYDINTKVMKDQYSLYEIMSAKEFYDANPGQSRPGAVEDDYKVYVTQKGSNSV
                     RAFRKGESWYFPNGTRANDGNFIFGGQIVTPKYKNAINDIRDPNFDPNTSFEDYKRQI
                     NLMPRLAFSFPISEDANFFAHYDILVQRPTSNTLFTALNYYYFEDPAIRPGQVNNPNL
                     KPEKTIDYEVGFQQKLTNATALKLSAYYKEMRNMIQNRTLLYIPAPVSSYDTYDNIDF
                     GTVKGFSVGYDYRRTGNLMLNANYTLQFADGTGSDANSQAGLTQRGNIRTLFPLNFDE
                     RHTFNVNIDWRYESGKKYNGPRIRGIDILSDFGINIDARAISGRPYTRNLLPTPRGGS
                     GFAGAINGARLPWRFFLDMKVDKSINLNRSENRKRDLYLNIYLRAQNVLNLKTVLGVY
                     PVTGSADDSGYLVSPRGLQDISNVITSGRDPEAFIDSFQWYVLNPGNYAPPSRLYLGA
                     VFQF"
BASE COUNT         4914 a         3059 c         3343 g         4697 t
ORIGIN      
        1 ttatctttta gtgggttttg tggcgacact atcagccata cttcttttag gtcggtgtgt
       61 gtggccataa aattagcaat tatcaaatgc ccggtatgta cagggttgaa tgatccgaaa
      121 aaaaggccag ttttcatgta ttaataaaca gaagaatcag aattttcttc aatcatttcg
      181 tcaaaaaatt catccaggcc ctcatctagt tcttcaaaat ttcctccaaa cagccatttt
      241 tcatttactt ttttcaagta aacactatca tagatacctt cttcgtaggc aaatttgcat
      301 acagccgaat cttttattac acgacaactc aatgatttaa acaaatgtct ggacgtatcc
      361 ggctcttcac tgattttgct gattacattt acaagatatt gtccgttttc ggtactaagt
      421 tttttagctt cttcaaattt tccgttgtca atcaggtaga gccagttatt aagtgcggtt
      481 tccggacttt catctttctt gcaagcgaac aaggtgagac agcacattga taatacgaaa
      541 atgaaagacc cgattttcat agatgatatg gcggatgtta tataagttat tcaagaaatg
      601 taattactgt acgttgacca ttaccggcat tactagcata agcaagtcac ttgacggatc
      661 ctgctcagcc ggacgaatga cacctgccct gtttggttca gaaagctcaa gtgttacgtc
      721 tgtggatgaa agtatggtca gcatttctat taaaaacttt gcattaaaag ctatctgaat
      781 cggaacacca tcatacgtac atgtgatggt ctcctgagct tcatttgaga aatcaagatc
      841 ctgtgatgaa atgtgcaatt gttcgccatc tatttgtatc aatacctgat tggtggtctt
      901 attggcatag atagcaatac gctttaggga attcagaaaa tcggttctgt gaatggtcat
      961 caggtttgga ttattgaccg ggataaccat attgtagtca ggatatcttc cttctataag
     1021 cctgcatgcc atctggaagt gaccgaatgt aaagaatgca tttgatttgt tgaacgaaat
     1081 ccttacttcg tctgtgtttg acaaagaaga cttaatcaag tttagagctt ttttgggcat
     1141 aataaatgag gaggacacag cctcattgtc tatacttctt gagtatttga cgagcttgtg
     1201 tgcatccgtg gctacaaaag tagccttacc ttcttcaaac aaaaaatata cacctgtcat
     1261 tgttggccgt aggtcatcat tgctcgtcgc aaacagtgtg ttcgtaatag cggatgcgag
     1321 tatgtcagta ggtatgacaa tctcttcatc ggtaggagat tcgggtgtct ccggaaaatc
     1381 aaccccattt tcgcctgaaa gcttatatgt tccataggca ctagtaaggg ttacatggtt
     1441 cgaactttcg tccacttcaa agcttaccgg ttgctcaggc agtgctttaa gtgtctcagt
     1501 aagtatttta gccggtatag caatagaccc atctttctcc gctgtaatat caatgtgagt
     1561 aatgatagta gtgtccaggt cagaggctgt tatttttagc ctgttgtctt ttatggtaaa
     1621 taaaaagtct tccaatatgg gcaatacagg gttatttgta atggcaccac tggctatctg
     1681 gaggtgtttt agcagctgag aagaggatgt actgaatttc ataaagcggt tttgctttga
     1741 taagatgctg caaaaatatc tctatttaac tacaattaat aatttttaac agaaaatcac
     1801 tttatcaaaa ataatactta acgataatgc tgaaaatgaa taattttgac ccgcaatgga
     1861 tagaaaaata gcaccacctg taaaggatat cagaaaagtg atccttcctc gacctgaaat
     1921 ctgcacccta agcaatggta tcacaatatt tggtactcaa agaccaggcc tcccggtatt
     1981 aagattagaa atagtagtga tggcaggaag accatttgaa cggaagaaga tcatttcaaa
     2041 acttaccggc aaactgctca aagaaggcac cacaagtaaa tcatcaagga aaatcgcaga
     2101 tatattcgat aaatacggcg ccaccattca tacaagtgcc aatcttgact atgttaccat
     2161 ttctgtccat tgcctgagaa aatatttctt ccaactctgg ccagtagtct atgaaatact
     2221 acgtgagcct gctttccctg aagccgagct aaatacttat gtagctaata atatacagaa
     2281 attagagata gaccttacaa agcctgaagt agtggcttac agattgatta cagagaaaat
     2341 tttcggcaat gaccatccgt atggttacaa tagtactcct gaattatacc gtcaagctac
     2401 ccgggctgac attactgagc attatcaaag ggcttttatt ccttcaaaaa caaatataca
     2461 tatcagtggc ggatatgaag ctgaggacat agatttgatc gctgagtcaa tgggagcatg
     2521 gcagaatcca cacttgtcag aaccggtctt tgcaggctta ataagcgaca aaccaggtaa
     2581 tatccatatc gaaatgccta ctagtagcca agcttctcta cgtataggca gacggatgtt
     2641 ctccaggtcg cacccggatt ttattgatgt atttatattg aataccttgc ttggtggttt
     2701 ttttggctct cgcctcaata tgaatatccg tgaaggaaag ggcatgacct acagtattgg
     2761 atcttcgctc gatccactga gttatagcgg ctgtctgatt ataatggccg atgtgagcca
     2821 cgaacatata ggaaaagcaa cccgacaggt atttaaagaa tttaaaaaac tgagagaaat
     2881 accgattgtt aatgatgaat tgacacaatt gaagcgttat cttataggtt cacttattaa
     2941 tgcagttgac ggccccttcc actcatctgc catgataaaa tatttgattg cagaccaggt
     3001 agatctcaat atttgggaca aagcaaacga acgtatttgt gacatcacaa cagaggaaat
     3061 acaacatact gcacagcaat accttaaaca agaagactac tgggtagtaa gtgccggtat
     3121 aaatacctga ttaaatggaa gtttgcccga atctttttaa aaaaagaaaa tatttatcta
     3181 aggcgtatca atattaaagc gaatattaaa aaaatagtaa aaatttctgt tgttttgcac
     3241 tttgatacaa agtactacat tcaaaatcat catagaagac agtctgtaaa tgaaattttt
     3301 taattttttt actcaggaat tagcaataga tcttggtaca gccaatacgc tcatcattca
     3361 ggacggaaag attgttatca acgagccatc catagtggct attgaccgca aaaccggaga
     3421 aaccattgca gtaggtacca aagcaatgca gatgcacgaa aaaacccatg atagcatcaa
     3481 aactatccga ccactcaagg acggtgtcat tgctgatttt caggctgctg aaaacatgat
     3541 tgaaggattt atcaaaatga ttggtgtacg gaagaaattt tttacacaca tgaagatggt
     3601 tatctgcatc ccttccggta tcacagaggt tgaaaaacga gctgtatttg actcagccga
     3661 tcatgttgac tcaaaagaaa cctacctcat ccacgaacct atggcagcag cattgggtat
     3721 tgggctagat gtagaagaac cggtaggtaa catggttatt gacattggag gtggtactac
     3781 agagatagcc gttattgcct tgtcaggaat agtttgtgat cagtcaatca gaactgctgg
     3841 cgacgaattt acaatggata tcattgattt catgaaacgc gaatacagtc tgctcgtagg
     3901 agaacgcact gccgaacaaa taaaaataaa tgtcggcgcc gccatcgaag atcttgccaa
     3961 cccacctgct gattatgcag ttaatggccg tgatatggtc tcgggtattc ctaaacagat
     4021 aatggtaggt taccaagaga ttgcccgagc acttgataaa tcaattgcta aaattgagtt
     4081 ggctattctg aaagcactcg aaactactcc accggagttg gcttctgata tatatcagaa
     4141 agggatttat ctaacaggtg gtggtgcatt gttgagaggt ttggataaaa gaatctctga
     4201 ccgaacaaaa ttaaaagtac atatagctga tgatccgctt gcagcagtag tagcaggtac
     4261 aggcattgca cttaagaata cacacagatt ctcattcctg atcgacagga agagcgtctg
     4321 atgattgatg attacacaat attctgccgg atatgctgaa tgttatccaa ttcattatca
     4381 gatttggaac attttttctg ttcttgggat tagagatagt ctgcatttac cttattgtca
     4441 attataataa ggcacagcaa gatatatttc tgaattcagc aaatttgctt tccggttctg
     4501 taaacgaaaa gtttacaaaa gtgaggagct ttatgaatca gtattccgta gtggatagta
     4561 tttctcaaga aaatgccgca ctctatcaag aacggttcaa ttatccgttt ttatttgata
     4621 cacttgataa cttcaaagat acaagtttgg tttattctgt aataccggca cgtgttatca
     4681 ataattcggt gagtacacaa cacaattatt ttaccataaa taagggtact tctgacggcg
     4741 tacaaaaagg ccttggtgtg ataactaaaa atggggtagc aggaattgtc aaaagtacaa
     4801 gtaagcattt tgcactggtt atttcaatat taaatgatga tctcaaaact tcagcatcac
     4861 taacacgcaa cggatatttc ggctcattgg tatgggatgg caaggaccca aaatattttg
     4921 acctgcagga cctaccccgg catgccgaaa caaaggtagg tgataccata cagacaagtg
     4981 gttattctct tattttccca aaaggaatat tgctgggcac cgtaaaagaa atcaagcaaa
     5041 aggaaggaac caatttttat tctcttacgt gccggatatg ggaagatatg agcaagcttg
     5101 atatagttta tgtactcaaa aataatgcaa aacccgaact cgaacaatta gaaaaggatg
     5161 tctatacaga taaataaatt gcatttcagt ttattaagga tttttctgat tattgccatc
     5221 caggtacttg taatcaagaa tatcagattg ccatgggaca aggaaggcta cgggcttttt
     5281 ttgttttacc cggtggcaat aatgtttttg ccatctcagt tatcgaagcc cattaccttg
     5341 attattgcat tttgtgccgg cattacggtt gatgtatttt atgacacccc cggagtaggt
     5401 gcaggtgcgc ttgtagccat ggcttacgct cgcccgctgg cattcgaagt gtttgaggtg
     5461 cgtttgggta ttaaacttgg gcaggaatca cacaacctca atcctggttt tttcacgcta
     5521 ttgatgtatg ctgcggtact attgctcatc ttttgttttt gttatttttc atttgaatat
     5581 tttactattt actacattga ggacataatt ataaagacaa tctattcgtt tttattatca
     5641 cttactattg ttgctttgta tatcgttatt ttcaggccta aaatctgaga aacagtgaaa
     5701 gaattatttc agatcagaca gtattatgta atgggaatta ttgccttgtg tgggctaata
     5761 attgtaataa aactggctca gatccagatt tttgacaaca gttacagcaa cagagcaaat
     5821 gcagttacca ttgcaagaca gtatatttat ccttcaaggg gccttatcta tgataggaat
     5881 aacaaactcc ttattaataa tgatcctgta tatgaactct cggtagtagt aagtgaaatg
     5941 gatcctgcga tggatactgt caaactttgc aacctccttc aggtgagtaa acaagagctg
     6001 gatgaaaagt taaacattga ctggcgtgac cacagatttt caaaatcatt gccggttgta
     6061 ttatttcgcg atatatcacc ggctactttt gcagcacttc aggaagtact tttcgaattt
     6121 ccgggtttct ctgtacaaca acgtaaccgg cggacttacc ctttcccgaa tgcagccaat
     6181 attcttgggt atattgccga ggctaatgag aaacaaatcg atagctcaaa cggcaaatat
     6241 caaccggggg acgaggtagg tgttacgggt cttgaaaaat attttgaaga agagctacga
     6301 ggcagaaaag gtgtcaggta tgaaatgaaa aataatgttg ggaaaagcat cggagcctat
     6361 aaagaaggta aatttgatat agtggcttca gctggcaagg atattatttc atcaatagac
     6421 attgacctgc aggcttttgc tgaaaaacta ctgcataaca agaccggaag tatagtagct
     6481 attcagcctt caacaggaga aattcttgca atggcatctt ctccttcata cgacccaaac
     6541 cttcttgtca tgggcaaaga ccgcggcaag gcatttcagc aattgtcgca agatacgctt
     6601 aaacctttct ttgacagggc aatcatggca aagtacccgc cgggctctat tttcaaaaca
     6661 ctggtcggcc taatctccct tgaggaaggc cttactaccg ccaaccgtta tataccatgt
     6721 aacggcgcat attattatgg tgcaataaag tggggttgtc accaccacat ccatgccact
     6781 tctctcccca ttgcactcca agtatcctgc aacacatact tctttacact taccagagaa
     6841 atcatagacc agtacagctt tcacttgccg ggcaaaggac tgaatacttt tgctgaacac
     6901 ctgcatgact ttggccttgg tgcacccatc ggtatcgatt atcccaccga aaaaggtggt
     6961 aatgtacctt ctgctgcata ctacgataaa ttgtacaaac gacgtcgtgg atggaggtcg
     7021 cctaccatca tgtctatcgg catcggccag ggtgaaattc agctgacaac gcttcaaatg
     7081 gctaaccttg ctgccatcat tgccaacaag gggagttact atgttccgca cttggtcaaa
     7141 ggatatgcgg acggaacgac aaaaatcgaa gaacggttcc ggaccaaaca ttctgtcaaa
     7201 attaggcctg aacatttttt gcctattatt gaaggtatgg agcgggtcgt ctggtatggc
     7261 accggacgta gggcagccat tccgggtatt aattttgccg gaaaaaccgg tacttctcaa
     7321 aatccgcatg gtgaagacca cagtgtattt tttggctttg cccctgccga aaaacctgaa
     7381 atagccatag ctgttattat tgaaaatgca ggtaaaggtg ccgaatttgc cgcacctatt
     7441 gccagccttg ttgccgaaaa atacctgcgc aaagaaatat ccagaccagc tctggccgag
     7501 agtatggaag ccatcaatac catacgtatc aaaccaaaga ggaaaagact accataccgg
     7561 acatctgttg caatagattc tctacaaagt ccaccattgg ctccgtccga agaacccatt
     7621 caagcacaaa atgaagagaa acaatgataa tatcaactat agtggcagta tccgaaaacg
     7681 gagtcattgg aaaaaacaac caactacctt ggcatctgcc agccgacctt aaatatttta
     7741 aaaaaattac taatggccac catgtgctta tgggaagaaa gtgtttcgaa tcaattggaa
     7801 aaccactgcc cggcagaacc aatctagtac ttacacgcaa taataattat caaccggatg
     7861 gtattgttaa aatccacact attgatgatg gtataaatat agccaaagaa catggtgaga
     7921 ccgagttgtt catacttggc ggagctgatg tgttcagact gaccatgcct gttattgaca
     7981 aactttactt caatactatc catgctgagt ttgaaggtga tacattctgg cctaaaaccg
     8041 attggacaca atggaagctt gactcaacag aataccatga tgcagatacc aacaatcttt
     8101 tcccgtactc cttttcagta tttactcgca ggaagtgaaa gaagatcttc tgcaatattt
     8161 ctggcgaacc cacaattgga aagaccgatt gtacgtgaca acaaacggtt tacctataca
     8221 tattgtaaca ccaggtatcc ttaatcatga tgccgggccg gactttagca atgcaaaaat
     8281 cagaatcggt gatgagctat gggtgggcaa tgtagagatt catgttaggt catctgagtg
     8341 gtatatacac cgtcatcagt atgatccggc ttaccgaacg gtcatcctgc acgttgtttg
     8401 gaccgaagat cagccggtct ttatggatga aaatataaga atcccgtgcc tggcgcttaa
     8461 aagcattata tcgaaggaaa tacttgacaa ctatgaacga ctgatgaata atgccttgtg
     8521 ggttccgtgc cagaatgtag ttaatcgttt tgatatggag aaaggccttc ttgggatgaa
     8581 tgcgcgcctt gttgaaaaat ttgaagacaa atcggctgta cttcagcata tttttgacaa
     8641 aacaaagggt gactggaacg aaacactgca ccgattcata gcacgtggct ttgggctcaa
     8701 tgtaaatggt gatgcatttg aattgcttgc cgaaaaaatg aattatcagc ttacacaaaa
     8761 gtaccaacat gaccctgtta agcttgaggc aattgcattc tcagcatccg gacttatacc
     8821 tgacggtaat tctgatcata ctgaaaaact ctggcaaatc taccaaacac ttccacataa
     8881 tgatagaccc acaccacttg ctcaaggtgt ttttaaattt ttccggactc gaccatccaa
     8941 ttttcccggg cttaggattg cccaatggtt aaaatttctg agcaacccgg gctcactgtt
     9001 tgacacagtt ataaatctca attcgccaat cgaattttct gccatcattt gtcagccatt
     9061 ggacgaattt tggaaaaatc atttcagttt tactgccgag gctaagaact tttctactca
     9121 ggccggcaaa gattttgccg atttgctcct gataaattct atagctccat ttttattctt
     9181 ttatggtaaa ataacggaca actacgagct acgtcaaaag gcagtagatc tactggatgc
     9241 aatgaaagca gagagcaata gcgttattcg cggatttcat cagcttggtt tccctatcaa
     9301 ctttgcatca cattctcagg catttttatt gctgaaaaag aagtattgtg accgaaaacg
     9361 ttgcctcgaa tgccctttcg gtgttcagat attcaaataa gagtatcaat aatctttgtg
     9421 acgttcccag taattctgat catttatttc atccagtaac tgcaggtaat tctggtagcg
     9481 aaggtgatgt atagtgcccg attctacagc ctgtttaaca gcgcaacctg gttcgttaat
     9541 gtgtttgcag tcggcatatc tgcacgctgt tgagacttcg aatatttcgc gaaaattatg
     9601 agcgatgtct gttggttcaa gatggttgaa agacaacgtc ttgattcccg gtgtgtcaat
     9661 gatattggca ttaaatgaag tttggtgcat ttcagcgaag gtcgtagtat gttggccttt
     9721 gcctgaatgg tcagaaatag tatcggtgct aagtgtcaga gttggctcga tgcccataat
     9781 aagggaagat tttccgacac ccgactgccc ggataccaag gttgttttgt tgtttaacaa
     9841 tgattttagc tcttcaaagt tttctttact tacagtagaa gttttcagga caatataaga
     9901 cagagatgta taaagtgcct tgagttcttc gtagatttct gtttcgaccg gaccaaagag
     9961 atcagatttg ttgaatacta tgatgaccgg tatgttgtat ggttctgtca tcagcagaaa
    10021 gcggtcgatg aagccttgtt taagatttgg ttgtatgatg gtggtaataa ggcaggcctg
    10081 gtcaacattg gcagccagca gatggagttg atgtttttgt ctgggtgatt gtcttacgat
    10141 gtagtttttt ctagggagta ttcgtttaat aacaccctga ccggcactta tttctggctc
    10201 gacctgtaca taatctccaa cggctaccgg gttggtcagt tgccacccaa agagtttgtg
    10261 tttgccggtg atacggcatt tccattcccg attatcctca agtctcacct ggtaccagct
    10321 tccggttgac tttataacga caccattaag cattgaagtc tggttttttg acagatttgc
    10381 cagctgactt tattgcagct gcaaaatctg gtatcattgt gggatctttt tcaaaatgat
    10441 tcccgacaat tatcaggtca gcacctgctg aggctgtaac aaatgcttgc tcaggagttt
    10501 ttattccgcc acctactatg agaggaagct cgatggtaga tattactgtc tgtatcattt
    10561 caataggaat aggcctgata gcaccgctcc ctgcatccat atagatcagt ttcatgccca
    10621 gatattgccc tgcgagtgca gtaaatgcac ctatctcagg tttgtgataa ggaataggtg
    10681 ttgtattact gatgtaagag acggttgtct gaatacctcc atctatcagc atatagcctg
    10741 taggtattat ttccaggttg agcttgtgca gcaatggtgc tgcttcaaca tgcttgccga
    10801 taagcaattc aggatttcgc ccactgatta atgatagaaa caaaagagca tgtgcatgtg
    10861 gagaaacttg gtaagtacca gatggaaata agataagcgg aatattagtc agtttcttca
    10921 gtatctgcac caattcatca aaccgatgtg tggtcagcag acttcctcct acaaggatgt
    10981 aatcaacagc agctttttct aacagttgta tcagtgcagg tagttgcgat atatcagttt
    11041 tatcaggatc tagtaagatg cccagttctt tgttcccgtt ttttttcgat ataatgagtt
    11101 gttgtaaaat agcttccgac atccgtcagt tttttacaaa gttagtgttt tgggttcaat
    11161 tttatctatt ttcggtataa tgttaatagt tttgatggtt attttgcttt aaatatattt
    11221 ttgatgaaga aaattctcat tacagacctg gttcatgagt tattaataga aggttttgaa
    11281 aaagccggtt atacggtaga ttattttcct gacatcagat atgatgaggt gtgccggatc
    11341 atcagcaatt acttcggctt gatagtcaat agtaagatct tggtagataa agaatttata
    11401 gacaaagcag ttcgattaac attcgtgggc aggcttggtt cgggcatgga aataatagac
    11461 cgccaatatg ccgaacatag aggaatagga gtcttcaatt cgcctgaagg aaattgcaat
    11521 gcagtcgcag aacatgcact tggaatgttg cttgcccttg ccaataatct tgtctgtgcc
    11581 gaccgtgagg tgagagaatt tgtctggaaa cgcgaaaaga atcgaggttt tgaaatagat
    11641 ggcaaaagta taggcatcat aggatatggt tataccggaa tggcctttgc caaaaaacta
    11701 ttgtcctggg atgtagaatt gcttgtacat gacaagtaca aaacacacat tgatacccag
    11761 tcaggtagaa ttaaagtcgt tgaaaaagca gaattgttaa agtatgcaga tattatttca
    11821 ttacacttgc ctcttacttc agaaacggtt agttttgtag atgagaaatt tattgagcag
    11881 tgggagaaga agggggttct catcaatacc agcaggggta aggtagtcaa aaccactgcc
    11941 ttgcttagcg ctttggaccg gggggagttg caaggggtat gccttgatgt gtttgaaaat
    12001 gaaaagcctg agacctactc ggctgatgaa aaaattttct atgaaaattt gttcagacac
    12061 aaaaattcaa tctttagtcc tcatattgcc ggctggacga tagaatcaaa agaaaaacta
    12121 tcaaaaactt tacttaacaa aatattattt tttattgaaa gcaataattt ataatttaca
    12181 gaattttatt ttgaaacgaa aattaataaa atcaatccta tttcgtaaat gttaaaattt
    12241 tgtgaaaata tttgatgatg aggcactaca attttggtat ttttttttga cagtgatttt
    12301 aattttcata acattgcaac ctaaatgtga atttggattt ttttacgttc aattaattag
    12361 aatatgataa gacgtttatt gttatttgca attctttggg tagtggggac aggagtatat
    12421 gcccagactt cacttgaagg taagattaca gaggaagata caggtgcgcc tgctgagctg
    12481 gcaaccatca aattgtttaa aggcggagta cttgttaccg gtgctcagac tgattttgat
    12541 ggtttttaca gtatttctcc aattgacccg ggtacataca ccattgaggc cagctacgtt
    12601 ggttttacta ccaagcagat agaaggagtt gtcgtaaaac aaggcagggc gaacattgtc
    12661 aacatcaagc tttccaaagg tgttgtcatc aacgaagttg aaatcgttgc ctacaaagtg
    12721 ccactcatag agcaggataa cactactcag ggtgcagtag taaccgccga gcagataaaa
    12781 aaccttcctc agagaaatgt aaatgctatt gctgccagta cagccggtgt ttctacttcg
    12841 ggcgaaggtg gtagcatatc tatcagaggg tcgcgttctg ccaatactgt ttattatata
    12901 gatggtattc cggtaagtgg tagtttcgta ccggtgtggg accttgaaca gttgcaggta
    12961 atcacgggtg gtctgagtgc tgagtacggt gacgtgacag gaggtgttat ctcaggtgca
    13021 accaaagggc cttctcaaaa atttaccgga ggtgcagaag tagaaacatc agctcctttt
    13081 gataaatatg gctacaacct tgcaaatctc aatatcagtg gcccaatact gaaaagaaaa
    13141 aattcaaatg aatctatact tggataccgt ttttcgggta gctatacagg ctataaagat
    13201 tcaaacccga gtgcagtcgg tatttatact gccaagcaaa gcgtaattga caaaattgct
    13261 gctgatccta ttacaagaat caatgaaaac ttcctggccc cttcaggaga gttcttgaca
    13321 agtaaaaacg gagacgttga tctcgtaaaa gtaagaccga atgaggaatc aaagcgttat
    13381 gaccttacag gcaaactcga ctggaagata actaaaaaaa ttgatgttac ctttagtggc
    13441 acgtacagta aagattattc aaggtttaca ccaagtagtg cctggtacct actgaataca
    13501 caaaataatc ctgaatcatt ttcaaatatt tcaagatttg acttcagatt cagacataga
    13561 cttggtaatt ccaattatgc aaatgatgaa gaagccaaag cacagttatc agcattcaga
    13621 aatgcatcct acacacttca gtttttctat cagaatgcca attcgcttga gtatgattca
    13681 agatttaagg atagattttt tgactatggc cacatagcag agtttgactt tggttatcgt
    13741 ggagcgttag catttgatca ggacttcatt ccacgacacg tcgataatac aagatatttt
    13801 atcaaatacg acccgagcaa cagcaaaaat ccgggtttgg caaactataa caaagtaatc
    13861 aaagatgcca cttcagattt agattatata caacgtaatg gtcgtgcaaa tgattcttat
    13921 acgtctatat ggggtttcca taccaacgta aatgatattt ataacagata tgcaaaatcc
    13981 agatcagaaa gaagaacatt ccagggaaca ttaggatttg atttcgtacc cggaggttcg
    14041 gaaagaggta agcacaacat acagataggt gtattctatg aagagcgaat aagcagatcg
    14101 tatgctgtag caccaagagg gctttggaca gtggcacaac ttttacagaa ccaacacata
    14161 gcgggtgttg ataccagtct tgttgcagaa gctaatcata cattcatact tcctgacgga
    14221 tcacagatag tagcagattc gtttgctaac aaaatagacg accttgatgg tagattttac
    14281 aaagaagtaa gaaagacatt gggtatagga cttgaccatt atgttgaagt ggatcaaata
    14341 gatccttcat tactgagatt ggatatgttt agctcacgag agcttacaaa ccgtggtatc
    14401 gcttcattct atggttatga ttatctcggc aacgaattgt cttcaagtac aacgttcaat
    14461 gattttttca cttcgaaaga tgccaatggc aaacaactct tccctgttgc accggccaaa
    14521 ccaatttatg cttcgggtta tattcaggat aaatttacat ttaaggatat cattttcagg
    14581 attggtgtga gagcagatat gtacgacatc aataccaagg ttatgaaaga ccagtattca
    14641 ttgtatgaaa taatgagtgc caaggaattt tatgatgcaa accctggaca atctagacca
    14701 ggggctgtgg aagatgatta taaagtgtat gtaacacaga aaggatctaa ctctgtacgt
    14761 gcattcagaa aaggtgaaag ttggtatttc ccgaatggaa ctagagctaa tgacggtaac
    14821 tttatcttcg gcggacagat tgtaacgccg aaatacaaaa atgccatcaa cgacatcaga
    14881 gatccgaatt tcgaccctaa tacttcattt gaagattaca aaagacagat caacctgatg
    14941 cctcgtcttg cattctcttt cccgatatca gaagatgcta acttctttgc ccactacgac
    15001 atcctggttc aaagacctac ttcaaatact ttgtttacag cacttaatta ctactatttc
    15061 gaggatccgg caatcagacc gggacaagtt aacaatccta atcttaaacc ggaaaaaacg
    15121 attgactatg aagtaggctt ccaacaaaag cttacaaatg ctacggcact taagctttct
    15181 gcttattaca aagagatgcg taatatgatt caaaacagaa cgctgttgta cattcctgct
    15241 ccggtatctt cttatgatac atacgataat atagatttcg gtacagtgaa gggtttctca
    15301 gtaggatatg attacagaag aacaggcaac ttgatgttaa atgccaatta tacgcttcag
    15361 tttgcagatg gtactggttc ggatgccaat tctcaggccg gccttactca gaggggtaat
    15421 atcagaactt tgttcccgct caactttgat gaaagacata cattcaatgt aaatatcgac
    15481 tggagatatg aatccggtaa gaaatacaac ggaccacgta tccgaggtat tgatatcttg
    15541 tctgattttg gaatcaacat agatgccaga gctatttccg gtcgcccata caccaggaat
    15601 ttgttgccta caccacgtgg tggttcaggg tttgcaggag ccatcaatgg tgccagactt
    15661 ccttggagat ttttccttga tatgaaggtc gataagagca tcaatctcaa ccgttcggag
    15721 aacaggaaga gagacttgta cttgaatata tacctgcgtg cacagaatgt acttaatctt
    15781 aagacagtat taggtgtata tcctgtgaca ggctcggcag atgactccgg ttatctggta
    15841 tcaccaagag gacttcagga tataagtaat gttattactt ctggcagaga tccggaggca
    15901 ttcattgatt cattccagtg gtacgtgttg aatcctggca attatgcacc accgagtaga
    15961 ttatatcttg gagcagtatt ccagttttaa tgaagaaaat tatttgatta ata
//
LOCUS       DAOQQY010000033        14763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1322_length_14763_cov_6.430378, whole genome shotgun sequence.
ACCESSION   DAOQQY010000033 DAOQQY010000000
VERSION     DAOQQY010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14763
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1322_length_14763_cov_6.430378"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            544..990
                     /locus_tag="PLE23_03255"
     CDS             544..990
                     /locus_tag="PLE23_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82315.1"
                     /translation="MKKILAAIALTAIMMTTSFASTACDMCGKGDPAVELRGAMQKLW
                     ADHMQWTFSTVDAFFNNQKGLQANLDRLLQNQKDIGTAIFPYYGQAAGDALAKLLTEH
                     IQQAVPVLTAAQKNNQEGLKKALDNWYKNAQDIADFLSSANAKNWP"
     gene            1006..1212
                     /locus_tag="PLE23_03260"
     CDS             1006..1212
                     /locus_tag="PLE23_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82316.1"
                     /translation="MMKTHIDQTTAYSVDLLKGDYAAAVKKYDDANNHMKQMGEELSM
                     GIIKQFPAKFEKAKKPTAKSKSKK"
     gene            complement(1323..2756)
                     /gene="pyk"
                     /locus_tag="PLE23_03265"
     CDS             complement(1323..2756)
                     /gene="pyk"
                     /locus_tag="PLE23_03265"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMX82317.1"
                     /translation="MVPRLKHNTKIVATVGPASHSYESLLALAQSGVHIFRLNFSHGT
                     HDGHRSVIESIQKLNREYDFHLGILADLQGPKLRIGKMENEEMNIKPGDVLTFTNEKC
                     VGNLEKIYMSYEQFAEDVEPGEKVLVDDGKIVLEVLETNKKDNVKLKVLFGSVISSNK
                     GVNLPDTKVSLPCLTEKDLADLDFILTQPVNWIALSFVRSADDIIDLRKRVEARHHEA
                     KILAKIEKPEAVRRIKKIIKASNAIMIARGDLAVELPMEKLPGIQKEIINLCMQWARP
                     VIVATQMMESMIHNPSPTRAEITDVANAVLDGADALMLSAETAVGDHPPKVIEAMNKI
                     IIEAEKTFSVKGRRPTPNSKSRTFLSDVICFNAAKTAVDVGAQAILGLTISGYTAFKI
                     SSYRPDCKIYIFSERSLMLGTMNLLWGVQCFEYKKYTTTDETFEDITNILKTQNLLKH
                     GDLIVQTASMPIDRRLRTNMLKVCHVD"
     gene            2967..3206
                     /locus_tag="PLE23_03270"
     CDS             2967..3206
                     /locus_tag="PLE23_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMX82318.1"
                     /translation="MSNIAERVTKIIVEKLGVDPSVVTTEASFTNDLGADSLDTVELI
                     MEFEKEFDLSIPDEQAENIQTVGQAVSYIESQSGE"
     gene            3321..4568
                     /gene="fabF"
                     /locus_tag="PLE23_03275"
     CDS             3321..4568
                     /gene="fabF"
                     /locus_tag="PLE23_03275"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HMX82319.1"
                     /translation="MRRVVITGIGAVTPIGNNVSEYDASLRNGVSGAGLITHFDASNF
                     KTKFACEIKNFLPGDSLDFKEAKRMDLFTQYAMVAAHEAVLQSGFDLEKINLDNAGVI
                     WGSGIGGLKALQTEIAAFATGNGTPRYNPFMIPKMISDIAAGQISIKYGFRGINYCTT
                     SACASSSHAIGNSVDYIKLGKADVIISGGSEATVVEAGVGGFNAMKALSERNDDFLTA
                     SRPFDKDRDGFVLGEGAGAVVLEELEHAKRRGATILAEVTGSGMTADAYHITAPHPEG
                     LSVMHVMRLALKDSMMNKEDIGYINVHGTSTPLGDIAETKAIVGVFGEHAYNLNISST
                     KSMTGHLLGAAGAVELIAGLLGMLGNYIPPTINHFTDDPDIDPKLNLTFNTLQERKFD
                     AFMSNTFGFGGHNSSIVVKKYKE"
     gene            4572..5312
                     /gene="rnc"
                     /locus_tag="PLE23_03280"
     CDS             4572..5312
                     /gene="rnc"
                     /locus_tag="PLE23_03280"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMX82320.1"
                     /translation="MSIFRKIYNYYFSPSKDLARKIKSVAGFIPNDLTLFKLAFAHKS
                     SSNEKDYAIQNNERLEYLGDAVLGTIVAEYLFRKYPNANEGFLTKMRSKIVKRSSLNE
                     IADSMGLEIILSEYNSTKLSKAMLGNALEALVGAVYIETGYNYTYKYVVRRIMRKYLN
                     IHELESYDDNYKSQLLEWCQKNGKEVNYKVLSKYKHDKRDKFKIGVFIDGEQISSADD
                     YNKKAAEQTASELAIKQLQISVQKEEDQ"
     gene            complement(5397..5669)
                     /locus_tag="PLE23_03285"
     CDS             complement(5397..5669)
                     /locus_tag="PLE23_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMX82321.1"
                     /translation="MNKGDLVNNIAENAGITKVQATEALNATLNAIANGLKGGDKITL
                     VGFGTFSVSERAARTGRNPSTGKEIKIEAKKVVRFKPGKELDGNVN"
     gene            5849..6061
                     /locus_tag="PLE23_03290"
     CDS             5849..6061
                     /locus_tag="PLE23_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82322.1"
                     /translation="MKKKNFAFKVFISLIVPIIFAGISACSSSKTGCPANEKAHVKAD
                     KRGNLPTSRGKSSVFPKDVKKKLGVK"
     gene            complement(6054..7235)
                     /locus_tag="PLE23_03295"
     CDS             complement(6054..7235)
                     /locus_tag="PLE23_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX82323.1"
                     /translation="MNKQAITFKSMPFNMKVAVGFISLVLLFAIAGRFIANDVPIYYS
                     HKDWKGFPAMNKTSFLAFKQKNEDVEPDFQILPLIPYSATSIDYKHSLSKPGQDVGSR
                     HLLGTDTFNRDVLAGIIYGSRTALLIGILSNILAVIFGLFLGSLSGYYSSVGVVTGRQ
                     NFIFLLMIEILLGYGLLYNPYVSVYIYNTQVRLLLFFILTILSVYMLLRPQRKNFRLD
                     IDLLITKSVEIFQSVPGLLLLLGISAVTGTMDVVKLTLLVSLLRWPGITRLVRGEVIK
                     VRQSQFIESAKSLGVTDWRVIAKHILPNITRQIIVAFAFGVASSILLEASLSFLGLGL
                     PPDQVTWGMLLSQSRTNPAAWWLAIFPGLMISLLILACYTVGNYLNNRTGETSSKEEI
                     I"
     gene            complement(7232..8632)
                     /locus_tag="PLE23_03300"
     CDS             complement(7232..8632)
                     /locus_tag="PLE23_03300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX82324.1"
                     /translation="MSAFIIKRLLWGIPTLLVILLLCFLLLDHSPGKTVDTYMSNNFE
                     GVKSMENVEEYEYYYIQSVKKLGLDKPAFYFTLTNRAMLDTIGRITFLPKKTQAEKLS
                     ATSGNKKAVGEYFHTIDYLLNQVRRLTDTVFVQKNELTRELYNAKKDADYSEKLEQIS
                     QTIDDKNIASQLKNLLEQQKYFARNKPSASLLIPAIKWNGTDNRFHLMLTRLFSGEKN
                     ISLIDSRPVWLKIRESLRFTLAINLLAFILSVFVSYKLAIWLSMNAGRRVARSVNSLM
                     FVLYTMPAFWVGSMLLMFFSSSTYGSMLNWFPPGGVGDIGQGDNFFEIVGIRLHHYLL
                     PVICISYPVIVFLTTHLKDSIKDASGNQWAVTARAKGLSEVSTIKRHVLRNAIFPYIT
                     LLGNIIPSIFAGSVIMEVLFNIPGMGRLAYQSIISRDWPVLFNIILISGIITVLAQIL
                     TDILYAWLDPRINTGK"
     gene            complement(8643..10388)
                     /locus_tag="PLE23_03305"
     CDS             complement(8643..10388)
                     /locus_tag="PLE23_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMX82325.1"
                     /translation="MKKTPFNPIFLLLLLLVFPFCKSEKKVQVDVVTVRLESDPDRLN
                     PMLSSVGFATDVFRYMFYPLVDIDPATLELKPVLVTNLPVKSTVKGGENDGLTSYEFE
                     ILPEAKWDNGSDITANDFIFTLKAVFNPAVGAERWRALLSFIKDVKIDEKNSKKFKVV
                     TTEDYFLSDPILGSVNIYPEYLYDPNKLLRTFSIADLVNREKSAQLTDDPKLKQFAES
                     FTDAKYSRDPKFVSGAGPYILTEWQTGQQLVLEKKQNWWGDKLAGKYPMLTAYPQKLV
                     FRIIPDETAMMGFLRDGSLDVAGSISPSQYNNLNGDAQITSKYNIFTPTVLQYYYFAM
                     NNKDAILSDVKVRKAIAHLMDLDGAIGLLDGLGERITGPFHPSKPYYNHNLKPVPFDM
                     NTATTLLDEAGWKDTDKDGIRDKVINGKPTKLSVTISFGANSDIGKKLSLMLQENGKK
                     AGIDFVLDSREMSQVMGDVKAGKFQMSPLKNRLDPLPDDPFNSWHSATATGGGGNRVG
                     YSNPEADKIIEKIRTEYNVAERNKLYEKFQEILYNDQPVVFLFAPKEPVIISKKFPDA
                     KPTIIKPGFTLNYFH"
     gene            complement(10694..10954)
                     /gene="yidD"
                     /locus_tag="PLE23_03310"
     CDS             complement(10694..10954)
                     /gene="yidD"
                     /locus_tag="PLE23_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002452125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HMX82326.1"
                     /translation="MKYISGFFKFLFILPIRFYQWFISPLLGPKCRFNPTCSHYMVGA
                     IQEWGVLKGLWLGLRRISRCHPWGGFGDDPVPVNEKNQRKNT"
     gene            complement(11025..12338)
                     /gene="ahcY"
                     /locus_tag="PLE23_03315"
     CDS             complement(11025..12338)
                     /gene="ahcY"
                     /locus_tag="PLE23_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMX82327.1"
                     /translation="MQVTEKQLPYKVKDMSLADWGRKEIKLAEAEMPGLMSLRAKYQH
                     DKPLKGARIAGCLHMTIQTAVLIETLQALGAEVSWSSCNIFSTQDHAAAAIAATGTPV
                     YAWKGMTNEEFDWCIEQTLHAFKDGKALNMILDDGGDLTNMVFDKYPELVNDIKGISE
                     ETTTGVLRLYEREKNGTLSLPAININDSVTKSKFDNKYGCRESLVDAIRRATDLMLAG
                     KVAVVAGYGDVGKGSADSLRRAGVRVIVTEIDPICALQAAMEGYEVKKMDNAVKEADI
                     VVTATGNKNIVVDRHFKSMKHNAVVCNIGHFDNEIDMAWLNEHYGHTKDVIKPQVDKY
                     TLDGNDIIVLAEGRLVNLGCATGHPSFVMSNSFTNQTLAQLELWVNHDKYDNKVYVLP
                     KHLDEMVAQLHLEKVGVELETLTQEQAAYIGVPVEGPFKNDAYRY"
     gene            complement(12538..14400)
                     /locus_tag="PLE23_03320"
     CDS             complement(12538..14400)
                     /locus_tag="PLE23_03320"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HMX82328.1"
                     /translation="MKKSLEEVNASVNTTAIPGKLKRLLAFMGPAYLVSVGYMDPGNW
                     ATDIAGGSQFGYKLLWVLLASNLIAMLLQSFCARLGIVSGLDLAQASRTAYPRFVNYA
                     LYVLAEIAIAATDLAEILGMAIGLNLLFGIPLLWGVLISALDTFLLLFLLNHGIKKIE
                     AFIISLVAVIAFCFFIEMFLVQPDIHEVAKGLVPGLPSGAALYIAIGIIGATVMPHNL
                     YLHSSLVQTRKFERKSQDIKQAIRFNIIDSVIALNFAFFVNAGILILAGTVFYASGNH
                     QITEIQDAHKMLEPLVGNKLAPILFAIALIAAGQSSTITGTLAGQIVMEGYLNLRIQP
                     WARRLITRLIAIIPAVAVILINGEEGTGDLLILSQVILSLQLGFAVIPLLHFVGDKAW
                     MKEHVIGMLPKIAGWISVLLIVALNIRLVYEELHGWMSTGSLGFWKVFVLILAIAALC
                     LLFYIILFPTISKQRKQKPFIHAPLPPFAVKSVKSYKKIAITVDFSASDNIAINHALS
                     HGEKGVEYMLIHILESAGAFVEQDYINDAEAKHDQDLLDRYEADLRKAGYHVVTRLGF
                     GNPKKQIPAIVKEFNADLLIMGAHGHTAWKDMVFGTTLDAVRHRVSIPVLIVSE"
     gene            complement(14397..14732)
                     /locus_tag="PLE23_03325"
     CDS             complement(14397..14732)
                     /locus_tag="PLE23_03325"
                     /inference="COORDINATES: protein motif:HMM:NF022058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA domain-containing protein"
                     /protein_id="HMX82329.1"
                     /translation="MVKKAREGVMEFLLINHPLDCPICDQGGECDLQDQAMAYGVDFS
                     RFREPKRAALFLNMLDSLAISLGSLIEILECNEYDNSFLIKLNNKATVLISQKVAQNL
                     IVQPNNDRK"
BASE COUNT         4377 a         3165 c         2864 g         4357 t
ORIGIN      
        1 ccgctggcac acctgataca tctaagctaa tcttaggtgc agatgtgcgt tgtagaatga
       61 ttagctctcc aacagaattt cgcaactcaa ctgtattttt ccataccatt tgaaaaaatt
      121 atactgtcga ggtgtcgagg tgttttcata tctgattggt ttttatggat gatttaataa
      181 aatcaaaaat acaaaataac caacaatttt catacttcat tttccttttt tagcaataga
      241 tttatttgcc aaataacatt ttgaatacaa catcttattg acttttatgc agatgattat
      301 ttggcaatag ctaagtggta gttccagcaa gctttttagc tgcgcaattg accagttatt
      361 tcaacaaaat catagaagca attaaaatca actttgacca ttcaacccag aacactgtaa
      421 gttctgatga ttctagttta taaaaaaaat tcatttcgtt gtaacctttt cgtttttatc
      481 gactccatta ggatattact ctaaacaaaa aaatattctc aaacaattaa aaattaaact
      541 acaatgaaaa agattttagc cgctatagct ttaacagcta tcatgatgac aactagtttt
      601 gcttctacag catgtgatat gtgcggaaaa ggcgaccctg ccgtagaact cagaggagcc
      661 atgcagaaac tctgggccga ccacatgcaa tggacattca gcacagtaga tgctttcttc
      721 aacaaccaga aaggtctgca agctaacctt gaccgattgc tccagaacca gaaagacata
      781 ggcaccgcca tttttcctta ctacggacag gctgctggtg acgcacttgc aaaactattg
      841 accgaacaca tccagcaagc tgtacctgtg cttactgcag ctcagaaaaa caaccaggaa
      901 ggccttaaaa aagcacttga caactggtat aaaaacgctc aggatatcgc agacttcttg
      961 tcttctgcca atgctaaaaa ctggccgtaa aaagaaatga gggaaatgat gaaaacccac
     1021 atcgaccaga caacagctta ctctgtagat ttgctcaaag gtgattatgc tgctgctgtg
     1081 aaaaaatacg atgatgcaaa caaccacatg aagcagatgg gtgaagaact gtcaatgggc
     1141 atcatcaaac agttccctgc aaaattcgaa aaagctaaaa agcctaccgc aaaaagtaaa
     1201 tctaaaaaat aaagtacaat ccaaactgaa tagacacagc acaattggag aagtcatccg
     1261 gcttctcctt tttttgttct gataaaacaa aaaaaagata taggattagc ctggttttct
     1321 actcaatcta catgacacac ctttagcata ttggtgcgta gtctgcggtc tattggcata
     1381 ctggctgtct gtacgatgag atcaccatgt tttaataaat tctgtgtctt cagaatattg
     1441 gtaatgtctt caaatgtctc gtcagtggta gtgtattttt tatactcaaa acactgtacc
     1501 ccccaaagga ggttcatggt accgagcata agcgatcttt ctgaaaaaat ataaatcttg
     1561 caatcgggtc ggtaagacga gattttgaat gccgtataac ctgatattgt taagccaagg
     1621 atagcttgcg cacctacatc aacagctgtt ttggcagcat tgaagcaaat tacatccgat
     1681 aggaaggttc ttgatttgct gttgggcgtt ggtctccttc ctttaaccga aaaagtcttt
     1741 tctgcctcaa tgataatttt gttcatagct tctatcacct ttggcggatg gtcgcccacg
     1801 gctgtctctg cactaagcat aagtgcatcg gctccgtcca atacggcatt ggccacgtcg
     1861 gttatttcgg ctctggtagg tgacggattg tgtatcatgc tttccatcat ctgtgtggct
     1921 actattacag gtcttgccca ctgcatacat aggttgatga tttctttctg gatgccgggg
     1981 agtttttcca taggcaactc aacagcgaga tcgcctcgcg ctatcataat ggcattagaa
     2041 gctttgatga ttttctttat tctccttact gcttccggtt tttctatttt tgccagaatt
     2101 tttgcctcat ggtgtctggc ttctactctt tttctcaagt cgataatatc atctgctgag
     2161 cgcacaaatg acaaagcaat ccagttgaca ggttgcgtga gtataaaatc taagtcggcc
     2221 agatcttttt ctgtaaggca tgggagtgat actttggtat ccggtaagtt tacgcctttg
     2281 ttggacgaaa taactgaacc gaacaatacc ttcagcttta cattatcctt tttgttggtt
     2341 tctagtacct ccaacacaat ctttccatca tctaccaaca ctttctcacc cggctccaca
     2401 tcttcggcaa actgttcgta gctcatatag attttctcca gattgccaac acacttttca
     2461 ttggtaaaag tgagtacatc ccccggtttg atgttcatct cttcattttc cattttgccg
     2521 attctcaact ttggtccttg taggtcggcc aaaatgccca gatgaaaatc atattctctg
     2581 ttgagttttt gtattgattc aatcacactc ctgtgaccat cgtgtgtgcc atgcgaaaaa
     2641 ttaagcctga aaatatgcac accagactga gccagagcaa gcaaagactc gtaagaatga
     2701 gaggccggac ccaccgttgc cactattttt gtattatgtt taagccttgg aaccatattg
     2761 aagaaataat tgttatcaaa acgaaatgcc gaataaagag aggcaatttt aaagattttt
     2821 tttaacctca caaagaataa cacattaaac ggaataataa taaagaatgt ttaagtaaaa
     2881 aaaactgcat tccttttggt tatccaattt acatttccgt actttgcgct gttatttgat
     2941 tacaaataat taattttaat atcactatgt caaacattgc agaaagagta acgaaaatta
     3001 tcgttgaaaa attgggagtt gacccgtcag ttgtaactac tgaagcaagt tttaccaatg
     3061 accttggtgc tgattcgctg gatacagttg agctgatcat ggaatttgaa aaagaatttg
     3121 atctttcaat tcctgatgaa caagctgaga acattcaaac tgttggtcag gccgtttctt
     3181 atatcgaatc acaatcggga gaataatatt ctctctgatc tgtcccagat atatgtcgcg
     3241 gatataataa tccacaaaat ggcttgcgta gttgttttgt ggattttttt tagtttttca
     3301 taaccggtca gtaattttat atgagaagag tagtaataac aggaataggt gccgtaacac
     3361 cgattggcaa taatgtaagc gagtacgatg cttcacttcg caatggtgtt tcgggtgcag
     3421 gtctgataac gcattttgat gcgagcaatt ttaaaacaaa gtttgcatgc gaaataaaaa
     3481 acttcctacc cggtgactct cttgatttca aggaagctaa gcgaatggac ttgttcaccc
     3541 aatacgccat ggtagcggca cacgaagcag tattacagtc agggttcgac cttgaaaaaa
     3601 taaatcttga taatgcaggt gtcatctggg ggagcggtat cggtggtctt aaagcattac
     3661 agactgagat agcagcattt gcaacaggca acggtacacc gagatacaat ccgtttatga
     3721 ttccgaaaat gatatctgac attgccgcag gccagatttc aataaaatac ggtttccggg
     3781 gtataaatta ttgtaccact tctgcttgtg cttcatcttc acacgccatc ggcaactctg
     3841 ttgattatat caaactcgga aaagcagatg ttatcatatc tggcggatca gaggccactg
     3901 tagtagaagc cggcgtaggt ggattcaatg ccatgaaggc cctttcagaa agaaacgatg
     3961 attttttgac agcatcaaga ccatttgaca aagaccggga tggttttgta cttggtgaag
     4021 gtgccggtgc tgttgtactt gaagaactcg aacatgccaa acgtcggggt gctaccatcc
     4081 tcgcagaagt aacgggaagt ggtatgacag cagatgccta ccacatcact gctccgcatc
     4141 ctgaaggttt atctgtcatg cacgtgatgc gtctggcact gaaagattcg atgatgaata
     4201 aggaagatat cggttatatc aatgtgcatg gtacatctac tccactgggc gatatagcag
     4261 aaacgaaagc tatagtcggt gtgtttggcg aacatgccta caatcttaac atcagctcca
     4321 caaagtcaat gacaggccac ctgcttggtg cagcgggtgc agtagaactt attgccggcc
     4381 tgctcggaat gctcggcaac tatatccctc ctaccattaa tcactttaca gacgaccctg
     4441 atattgaccc gaagctaaac cttactttca atactcttca agagagaaaa ttcgatgctt
     4501 ttatgagtaa cacttttggt tttggcggcc acaattcttc tattgtcgta aaaaaatata
     4561 aagaataagg tttgagcatt ttccgcaaaa tatacaatta ttatttttcg ccgtccaaag
     4621 accttgcacg caaaattaag tctgtagccg gattcattcc gaatgacctg acattgttca
     4681 aactggcttt tgcacacaaa tcgtcttcca acgaaaaaga ttacgctata cagaacaacg
     4741 aacggcttga ataccttggt gatgccgtac ttggtacaat cgtagccgaa tatctattcc
     4801 gaaaatatcc aaatgccaac gaagggtttc tgaccaagat gcgctccaaa atcgtaaaac
     4861 gaagctccct gaacgaaata gcagacagca tggggctgga aatcatcctg agtgaataca
     4921 actctacaaa actaagcaaa gctatgttag gcaatgcgct cgaggcactt gtaggcgctg
     4981 tctatatcga aacaggatac aactacacat acaaatatgt agtacgtcgc attatgcgca
     5041 aataccttaa cattcacgag cttgaatctt atgacgacaa ctataaaagc cagttgctcg
     5101 agtggtgcca gaaaaacggc aaagaagtta actataaagt attgagcaaa tacaagcacg
     5161 acaaacgcga caaattcaaa atcggtgtat tcattgatgg tgaacaaata tcatcagccg
     5221 atgattacaa taaaaaagct gccgaacaga ctgcttcgga gctggcaata aaacaactgc
     5281 agatatccgt tcaaaaagag gaagatcagt aatttttttt gatagacaaa aaagctttaa
     5341 aaaagaaaag gcaacttatc gggataagct gcctttctat atagggacga aaaaaattag
     5401 tttacattac cgtccaattc cttacctggc ttgaatctta caactttctt agcttcaatt
     5461 ttgatttctt taccagtaga agggtttcta cctgtacggg cagctctttc gcttactgaa
     5521 aatgtaccaa aacctacaag tgttattttg tcacctcctt taagaccatt agctattgca
     5581 ttcaatgtag cgttcaatgc ttcagtagcc tgtactttag tgatgcctgc attttctgca
     5641 atgttgttaa ctagatcacc tttgttcatg ttaaaagttt tttttattta taaaataaag
     5701 tttaataatt gagtacgccg cttgggctct ctcatttaac ggcacaaaaa tgatgctttt
     5761 tgtaataata tcaagctttt tagagatttt tttcaatttt tttatcaatt ttaggtcatc
     5821 tttgcgttta atgataaata gcagatgtat gaaaaagaaa aatttcgcat ttaaagtctt
     5881 tatctcttta atagtaccaa taatttttgc cggtatatct gcttgttcaa gttccaagac
     5941 tggttgccct gccaacgaaa aagctcacgt aaaagctgat aaacgtggta acctgccaac
     6001 ctcaagaggc aaatcgtccg ttttccctaa agatgttaag aaaaaactcg gtgtcaaata
     6061 atttcttcct tactgcttgt ctctcccgtt ctattattca aataattgcc aacagtgtaa
     6121 caagccagta taagtaaact gatcattaac cccggaaata ttgccagcca ccatgcagcc
     6181 ggattggtcc gtgactgact caatagcatg ccccacgtca cctggtcggg tggcaaaccg
     6241 agtcccaaaa acgacaatga tgcctcaagc aaaatggaag atgctacacc aaacgcaaat
     6301 gctacaataa tctgtctggt aatattgggc aaaatgtgtt tagctattac cctccaatct
     6361 gttactccaa gcgactttgc actttcgata aattgacttt gcctcacctt gataacctct
     6421 cccctcacaa ggcgtgtaat acctggccac ctcaataaac tgacaagtaa tgtgagtttc
     6481 accacatcca tcgtacctgt cacggccgat atgcctaaca acagaagcag tcctggtact
     6541 gattggaaaa tttctaccga tttggttatt aataaatcta tatctagacg aaaattcttt
     6601 ctctgaggcc tcaataacat gtaaaccgac agaatcgtga gtatgaagaa caagagaagc
     6661 cttacctgag tattatagat atatacagat acataaggat tatacaatag accatacccg
     6721 agcagtattt ctatcattaa aagaaaaata aaattctgtc tgcctgtcac gacgccaacg
     6781 ctgctgtaat acccgctgag actcccaaga aaaagaccaa atatcacagc taatatattt
     6841 gataaaatac cgattaacaa tgctgtccta ctaccgtaaa tgatacctgc cagtacatct
     6901 cggttgaatg tatcggtacc gagaagatgc ctgctgccga cgtcctgccc gggcttcgaa
     6961 agcgaatgct tatagtcaat cgaggtcgcc gaatagggta tgagtggtaa tatctgaaaa
     7021 tcgggctcta catcttcatt tttttgtttg aatgccaaaa aggaggtctt gttcattgcc
     7081 ggaaatcctt tccaatcctt atgagaataa taaatcggca catcattggc aataaacctg
     7141 ccagctatgg caaaaagtaa gaccaagctg ataaatccta cagctacttt catattgaat
     7201 ggcattgact taaatgttat agcttgcttg ttcatttgcc tgtatttatt cgcggatcga
     7261 gccatgcgta taatatatct gtaagtatct gtgccagtac tgtaatgata ccgctgatca
     7321 gaatgatatt gaacaataca ggccagtcgc gactgatgat gctttggtag gccagtctgc
     7381 ccattccggg aatattgaac aaaacttcca taatgaccga acctgcaaaa atggaaggta
     7441 taatattgcc aagtaaagtt atatagggga aaatggcatt cctgagcaca tgacgtttta
     7501 tggttgatac ttcagataat cctttggctc ttgctgtcac agcccactga ttgccggagg
     7561 catccttgat actatcttta agatgggtcg taagaaaaac gatgaccgga tatgaaatac
     7621 aaatcacagg taacagataa tggtgtagcc tgataccaac aatctcaaaa aaattatctc
     7681 cttgaccgat gtcacctaca ccgcccggag gaaaccagtt gagcatactc ccataagtgc
     7741 tgctactgaa aaacatcagc aacatcgaac ctacccaaaa tgcaggcata gtatatagta
     7801 caaacataag gctattcacc gacctcgcaa cgcgtctgcc agcattcata ctcagccata
     7861 tcgccagttt gtatgacaca aacaccgata atataaaagc tagaagattg atagccaatg
     7921 taaaccgaag cgattcacgg attttcaacc agaccggtct tgagtcaatg agtgaaatat
     7981 tcttctctcc tgagaaaagc cttgtaagca tcaggtgaaa tctgttgtct gttccgttcc
     8041 attttatagc cggtatcagc agactagcgg atggtttgtt tcttgcaaaa tatttttgtt
     8101 gttctaataa atttttcaac tgagatgcaa tgtttttgtc atcgattgtc tgagaaattt
     8161 gctcaagttt ttcggaataa tcagcatctt ttttagcatt atataattcg cgggtgagtt
     8221 catttttttg tacaaaaacc gtatcggtga gcctgcgtac ttggttaagc aggtagtcga
     8281 ttgtatgaaa atattcacca actgcctttt tattgcctga tgtagctgag agcttttcag
     8341 cttgagtctt tttcggcaga aatgtaatac ggcctatggt atcgagcata gccctgttag
     8401 tcaatgtaaa ataaaaagcc ggtttgtcaa gtccgagctt ttttacgctt tgtatgtagt
     8461 aatattcata ttcctctaca ttctccatcg acttgacacc ttcgaaatta ttactcatat
     8521 aggtatcgac cgtcttacca ggcgaatggt caagcagcaa aaaacaaaga agtaaaatta
     8581 ccagcaatgt tggtatgccc cacaacagtc ttttgatgat aaaagctgac atggggttga
     8641 agttagtgaa aataattcaa agtaaagcca ggtttgataa ttgtaggctt tgcatccgga
     8701 aatttcttcg agataataac aggctccttt ggtgcaaaca agaatacaac aggctgatca
     8761 ttatacagta tttcctgaaa tttctcataa agtttattgc gttcggctac attatattct
     8821 gtccggattt tctcaataat tttatctgct tcaggattcg aatacccaac tctgttgcca
     8881 ccaccacctg tagctgtggc actatgccac gagttgaacg ggtcgtcggg cagtgggtca
     8941 agcctgtttt taagtggcga catctggaac ttgccggctt tcacatcacc catcacctgg
     9001 ctcatctccc ggctgtccaa tacaaagtct attcctgcct ttttaccatt ttcctgtagc
     9061 ataagtgaca gctttttacc tatatcacta ttcgctccga atgaaatagt tacagatagt
     9121 ttagtgggtt ttccattgat taccttatcg cggatcccgt ctttgtctgt atctttccaa
     9181 ccggcttcat ccagcaaggt tgtagctgta ttcatatcaa aaggaacggg tttcaggttg
     9241 tgattatagt atggcttgct tgggtgaaaa ggaccagtaa tacgttctcc cagcccatcc
     9301 agcaaaccga tggcaccatc caaatccatc aggtgcgcta tggctttcct cacttttacg
     9361 tcggagagta tcgcatcctt attgttcatg gcaaaataat aatattgcag gacagtaggt
     9421 gtaaaaatat tgtactttga tgttatttgt gcatcaccat tcaggttgtt gtattgagaa
     9481 ggtgaaatag aaccggctac atcaagacta ccatcgcgga ggaatcccat catagccgtt
     9541 tcatccggaa taatcctgaa cacaagcttt tgcggataag cagtcaacat cggatatttt
     9601 ccggcgagct tatcacccca ccagttttgt ttcttttcaa gtaccagttg ttgtccggtc
     9661 tgccattcgg taagtatata tggccctgcg cctgatacga acttcggatc gcggctgtat
     9721 tttgcatccg taaaagattc ggcaaactgc ttcagctttg ggtcatctgt cagttgagca
     9781 gatttttcgc gattgacaag atctgcaatg ctgaatgtcc ggagcagttt attgggatca
     9841 taaaggtatt ccggataaat attcaccgaa ccaagtatcg ggtcagacaa gaagtagtct
     9901 tcagtagtga ccactttaaa ctttttcgaa tttttctcat caatctttac atctttgata
     9961 aatgagagca gtgcacgcca acgttctgct cctactgcag ggttgaacac tgcctttaat
    10021 gtaaaaataa aatcattagc tgttatatcg ctgccattgt cccatttggc ctccggcaat
    10081 atctcgaatt cgtatgatgt aagtccgtca ttttcaccac cttttacggt tgatttgacc
    10141 ggcaggtttg taacgagtac gggcttgagt tctaatgttg ccgggtcaat atctaccagc
    10201 ggataaaaca tataacggaa cacatctgta gcaaaaccaa cagaagaaag catgggattc
    10261 aacctgtcag ggtccgactc tagcctgacc gtgacaacat cgacctgaac ttttttttct
    10321 gatttgcaaa atggaaatac cagcagaagc aataatagga aaatcgggtt aaagggagtc
    10381 tttttcattc atcgggatgg tttagaaatt tcagaacgta aaattaattt aaaatggaat
    10441 atttgctcct gaaatcaatc tttttttata ttagctgaaa attgaatggg tttaaaacaa
    10501 aatcagctgc caaggctatt aaacccggaa tatgcattag gcgctttatt gcaaatcatt
    10561 attccttcaa caaccatttt aaaagctcaa aaaccattgt tcactatacc tttatcactt
    10621 tttgtggctc ttgtgtacga aacattttcg tttaaattct taattggtta tctgggtcaa
    10681 taccaagtct gatttatgta ttttttcttt ggttcttttc attgactggt acagggtcat
    10741 caccgaatcc gccccatggg tggcatctgg aaattctcct gagtcctaac cataatcctt
    10801 ttaacacacc ccattcttga atggcaccca ccatataatg cgagcaggtg ggattaaatc
    10861 ggcatttcgg acccaacaga ggcgaaataa accattgata aaacctgata ggtaggataa
    10921 acagaaattt gaaaaacccg gaaatatatt tcatttcatt atgaataaaa gccacctgct
    10981 aaagttaaca agtggctttt aaaaaagatt cgaattacaa gctattaata tctgtaagca
    11041 tcattcttaa acggaccctc gaccggaacg ccgatgtatg ctgcctgctc ctgagtaagt
    11101 gtttcgagtt cgacccctac cttttcaaga tgtagctgtg cgaccatttc atcaagatgc
    11161 tttggtagta catatacttt gttgtcgtat ttgtcatggt ttacccatag ttcaagctga
    11221 gcaagggtct ggtttgtaaa agagttactc attacaaacg atgggtgacc ggtggcacaa
    11281 ccaaggttga caagccttcc ttcagcaagt acgatgatgt cgttaccgtc aagtgtatat
    11341 ttgtccacct gaggtttgat gacatctttt gtatggccat aatgttcatt gagccatgcc
    11401 atatctattt cgttatcgaa gtgtccgata ttacaaacaa ctgcgttgtg cttcattgac
    11461 ttaaaatgtc tgtcaacaac aatgttttta ttgcctgtgg cagtaactac tatatccgcc
    11521 tccttcacag cgttatccat tttctttact tcatagcctt ccatagctgc ctgtagggca
    11581 cagatagggt caatctcagt aacgattacc cttacacctg cacgtctgag tgaatcggca
    11641 gaaccttttc ctacatcacc gtatccggcc actacggcta ctttaccggc gagcataagg
    11701 tctgtagctc ttcgtatagc atctaccaat gattcgcggc agccgtattt attgtcaaat
    11761 ttgcttttcg taacagagtc attgatattg atggcaggaa ggctgagtgt tccgtttttt
    11821 tcacgttcgt aaagtcggag tacaccggtg gtagtctcct cagaaatacc tttgatatca
    11881 ttgacgagtt cgggatattt atcgaaaacc atattggtaa gatcgcctcc gtcgtccaaa
    11941 atcatgttga gtgccttgcc gtctttgaat gcatgcagcg tctgttcgat acaccagtcg
    12001 aactcttcat tggtcatacc tttccatgcg tacacaggtg ttccggtagc tgcaatagct
    12061 gctgctgcat gatcttgtgt agaaaaaata ttgcaggatg accatgatac ttcagcacca
    12121 agagcctgaa gggtttcgat caatactgcc gtctggatag tcatgtgcag acaaccagct
    12181 attctggcac ctttgagtgg tttgtcatgt tggtattttg ctcttaatga catcaatccg
    12241 ggcatttctg cttctgccag ttttatttct tttcggcccc agtctgcgag gctcatgtct
    12301 tttactttat aaggaagttg tttttcagtt acttgcatgt aatctgtata ttttgtgaat
    12361 aatgtgaata attaaaaaac cggtgcaaaa ttacaacaat taacccatga tttcaactat
    12421 tgttgtgttc ctgtcttact ttatgaaaca taagcttctt tttttgccaa tcaataacat
    12481 caaggcttgt gccaacaaaa acattttttg ctaaattgca gtacgggttg atcaaactta
    12541 ttctgataca atcaaaaccg gtatggacac tctgtgccta accgcatcaa gggtggtacc
    12601 aaataccata tccttccatg ctgtatgtcc gtgtgcaccc attatcagca aatctgcatt
    12661 aaattcctta actattgccg gaatctgctt tttcggattt ccaaaaccga gtctggtgac
    12721 tacatgatat ccggcttttc gcaaatctgc ttcgtacctg tcaagcaaat cctgatcatg
    12781 ctttgcttcc gcatcattga tataatcttg ctctacaaat gcacccgccg actccaggat
    12841 gtgtatgagc atatattcaa cacctttttc gccatgagac aatgcatgat tgatggctat
    12901 attatccgag gccgaaaagt caactgttat agctatcttt ttataagatt taacggattt
    12961 gaccgcgaac ggtggcagag gcgcatgtat aaagggcttt tgtttccgtt gctttgaaat
    13021 ggtcgggaac aatataatat aaaacaaaag gcacaatgca gctatggcca aaataagtac
    13081 aaaaaccttc caaaaaccca atgacccggt tgacatccaa ccatggagct cctcatatac
    13141 aagcctaata ttaagtgcaa caatcagtaa tacacttatc catcctgcaa ttttgggcaa
    13201 catacctatc acatgctcct tcatccaggc tttatcgcct acaaaatgaa gcaatggtat
    13261 caccgcaaac ccaagctgaa gactaagtat gacctgactc agtatcaaca agtctcctgt
    13321 accttcttct ccatttatca ggatgactgc tactgccgga ataatggcaa ttaatctggt
    13381 gataagtctt cttgcccaag gctgtatcct gaggttaaga taaccttcca ttactatctg
    13441 gccggccaag gtgcctgtaa tggtcgagct ctggcctgct gctatcaaag ctatggcaaa
    13501 aagtattggc gccagcttat tacccacaag cggttcgagc atcttgtgtg catcctgtat
    13561 ttctgttatc tgatgattgc cggatgcata aaataccgta cctgccagaa taagaatacc
    13621 tgcatttaca aaaaatgcaa aattgagcgc gatgacagaa tcaataatat tgaaacggat
    13681 agcttgtttg atgtcttggc tctttctttc aaactttcgg gtttgtacaa gtgacgaatg
    13741 cagatacaga ttgtgtggca taacagttgc tcctatgatg cctatggcaa tgtacagcgc
    13801 agcaccggaa ggtaatccgg gtactagacc ctttgctact tcatggatat cgggctgcac
    13861 aagaaacatt tcaatgaaaa aacaaaaagc aataactgcc accagtgaaa tgatgaatgc
    13921 ctctatcttc ttgataccat ggtttagtaa gaacaaaagc aaaaatgtat ccaatgcaga
    13981 tataagcaca ccccaaagca aaggaatacc aaaaagcaag ttaagcccta tggccatacc
    14041 caatatttct gcaagatcgg ttgcagcaat ggcaatctct gccagtacat ataatgcata
    14101 attaacaaac ctcggataag ccgtccttga tgcctgcgcc agatctagac cactgacaat
    14161 accaagtctt gcacaaaaac tttgcaacaa catcgctata aggttggagg caagcaacac
    14221 ccaaagcaat ttgtacccaa actgacttcc acctgctatg tctgtagccc aattgccggg
    14281 gtccatatac ccgacactaa caagataagc aggtcccata aacgcaagca acctcttcaa
    14341 tttgccaggg atagccgtgg tattgaccga tgcatttact tcttctaatg attttttcat
    14401 tttctgtcgt tgttaggttg tacaatcagg ttttgagcca ccttttggct tataagcaca
    14461 gttgccttgt tattcaattt tattaaaaaa ctgttatcgt attcattaca ttcgagaatc
    14521 tcaatgagag aacctaagct gatagcaagc gaatcaagca tattgagaaa taatgccgcc
    14581 cgcttcggct cgcggaagcg gctgaaatcg acgccatagg ccatcgcctg atcctgcagg
    14641 tcgcattcgc cgccctggtc gcagatcgga caatcgagcg gatggttgat gagcaggaac
    14701 tccatcaccc cttcgcgcgc cttcttgacc atcggcgaat tggtccggac gacgggcggt
    14761 tcg
//
LOCUS       DAOQQY010000034        14761 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1324_length_14761_cov_4.954236, whole genome shotgun sequence.
ACCESSION   DAOQQY010000034 DAOQQY010000000
VERSION     DAOQQY010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14761
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_14761_cov_4.954236"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..1702
                     /locus_tag="PLE23_03330"
     CDS             65..1702
                     /locus_tag="PLE23_03330"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HMX82330.1"
                     /translation="MKITLAQLNYHIGNFESNLRKMLDAVTLAKEEGSDIICFGELAT
                     CGYPPRDFLEFRDFIARCMDSVHALAAAAQGIAIAVGSPCVNPVVEGKDLYNSAFFLF
                     DGKVQHISHKALLPTYDIFDEYRYFEPAHEFSTVEYKGKRIAFTICEDIWNVGNDNPL
                     YKVCPMDEMMKDKPDIMINLSASPFSYDHAESRISAVRANVSKYGVPMFYVNHVGAQT
                     EIIFDGGSLVVSPDLQVFDEMPYFEECIKTYNLADVQKGGINNEQQKEKYRLMHDAIV
                     TGIRDYFGKLGLKKAILGLSGGIDSALTAVLAVRALGAENVWGVLMPSEYSTGHSVTD
                     AKDLAVNLGIRHDIVPIRSVFDVYEGLLNPLFEGKQADVTEENIQARIRGMILMALSN
                     KFGHILLNTSNKSEFAVGYGTLYGDMCGGLAVLGDVYKTEIYKLAAYVNKDGEVIPLN
                     SICKPPSAELRPGQKDSDSLPEYDTLDPVLFQYIENRIGPAELMEMGFDPAMVNRILR
                     LVNINEFKRFQAPPVIRVSNKAFGAGRRLPIVGKYLS"
     gene            1831..2730
                     /locus_tag="PLE23_03335"
     CDS             1831..2730
                     /locus_tag="PLE23_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX82331.1"
                     /translation="METVLEINNLTKVFGKITAVDSLSFSVNKGDIYGILGPNGSGKS
                     TTLGMVLNVVNPTSGSWKWFGEEPQQEHLKRIGAIIERPNFYPYLTATQNLKIVAKIK
                     NANPNNIEHKLRTVGLFDRKDSVFSTYSLGMKQRLAIAAALLNDPEILLLDEPTNGLD
                     PQGIRDIRDIIRQIATGGTTILLASHLLDEVEKVCSHVVVIKNGKKLYSGKVDEMTAS
                     YGYIEVNADNRTLLLQKLGELGFTKIQQEKDCIKLILESETDTRSLNETLFKSGVTLT
                     HLVKRKNTLEEQFIELTGQKMHA"
     gene            2727..3551
                     /locus_tag="PLE23_03340"
     CDS             2727..3551
                     /locus_tag="PLE23_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMX82332.1"
                     /translation="MKNLIQLELLKLWPNRSFRIYTLLYFCIIVGLFFLSVIEFNLGP
                     LKFSFGAQGAFKFPYTWHFNTYIASHFKILLALIIITNIVNEYDFRTIKQNFIDGLSK
                     KEFVTSKVLTFVILSLCSTILVGMIALVLGLKFTGNITAGMVFREMYYLPIYFLELIA
                     TFSFLMFAAVLIRKSAFALGFWIFWSTAESILRNVWKSKIPNTFNPFDFFPMQSISNL
                     IHEPFSRMETVQKISNMVQPGKVIDYSIQWPYVLMCLCWSGLFVYLSYYLIKKRDL"
     gene            3731..4180
                     /locus_tag="PLE23_03345"
     CDS             3731..4180
                     /locus_tag="PLE23_03345"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HMX82333.1"
                     /translation="MGYLENNLLGGESIYYQTHVHWTIYIKGLLSLIFGIFLISIEVD
                     SLGIIFILISFLILSMSKLKIHNSEIAVTNKRVLIKEGLITTQSLELMLNKIEAIHVK
                     QGLIGKMFNSGTIEIIGTGGTNSHFSNIFNPLEFRRAVNYQITKSNL"
     gene            complement(4583..4759)
                     /locus_tag="PLE23_03350"
     CDS             complement(4583..4759)
                     /locus_tag="PLE23_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82334.1"
                     /translation="MDKLYNSQQERIQQISVKHTKELFGGKIGLLFYDVTTLYFETEK
                     SDEFREPGFFGYSG"
     gene            complement(4799..5242)
                     /locus_tag="PLE23_03355"
     CDS             complement(4799..5242)
                     /locus_tag="PLE23_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82335.1"
                     /translation="MIWVAQRLKTLNLNMFVRKKRNKSGTFSVVIVDKSSGKYRELRT
                     IGVSSDESEINRLVAKGRKWIDQYSNGEDIFEKHEQEKEEKQIAIHLLDSIANIYLNG
                     IQLIVNYAYDSIGFNSIEDKELRSLVTARLAQPLSKNATVEYLRS"
     gene            complement(5579..6760)
                     /locus_tag="PLE23_03360"
     CDS             complement(5579..6760)
                     /locus_tag="PLE23_03360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82336.1"
                     /translation="MKRIFTICFLMFIGSLLHSQTPITINASDMPVPEGLYNFKNLLN
                     LAPAPVPQKNGTWDYSQTEGEDTLVLAYYKETLPVFTDEGVDVYQSGFKALTQTLGYN
                     VFYEIDFNDTAVLDKMMYIDNQAYGLGALTGNNKDSLTIPLQGWQWGSPRKIIQFPFT
                     DNSSWQSVSYRKTNFNLTVTAAALNKAPGVQSYYLVRLDTIVGYGKMRLHTPNGPSKY
                     YDVLVDQSSQFAIDSFFLFGQPAPAVLLNAFGITQGQVTGVDNRYIFYRKGSYVPLAG
                     VNYAADTFTTPTIFFYNTDDLLLSDENVNQQAFTTVLFPNPLKGTQLKMMVKGLDVSQ
                     NTYTITDFLGRIVQSGKVQANGELLSFDLSANLTSGQYIVSILDGLNQAIITESFIVS
                     H"
     gene            complement(6891..8018)
                     /gene="hemW"
                     /locus_tag="PLE23_03365"
     CDS             complement(6891..8018)
                     /gene="hemW"
                     /locus_tag="PLE23_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMX82337.1"
                     /translation="MAGIYIHIPFCKQACHYCNFHFATSHRYKNELVQALLIELELRK
                     AYLGPDATIETIYFGGGTPSLLTQAETMSIFDAVFKHYHTDLREVTFEANPDDLTAGY
                     LEALSQTPVNRLSIGVQSFFEEDLRWMHRAHTRQQAENCIRMAMDTGFNHLTIDLIYG
                     APTTTDAMWQHNMQRAIELGIEHISAYALTVEDKTPLSKMIRTGQAQTVNADKSATQF
                     VMMVKTLTEAGFEQYEISNFAKNRQYALHNTSYWQGVHYLGIGPSAHSYDGRSRCWNI
                     ANNRQYIKSVVNGIIPAESELLTTADRFNELIMTGLRTMWGVSLQALEQLDSSYATLF
                     QQNIQKYISAGQMKLESGSCILTQEGKILADGIASDLFVLT"
     gene            8044..9885
                     /locus_tag="PLE23_03370"
     CDS             8044..9885
                     /locus_tag="PLE23_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMX82338.1"
                     /translation="MVKFTFYSSMVAYSIEHTHHLPTNRLLSILFTGLLLLAGNHISA
                     QKRYILELQYDSGQKTMTRLFQDSLSAELFIQSEIKALMSKAHLEAAVDQKKYPNDST
                     TVATIHIGPEYEWAELAWSVTDPIFRKPANWDKKWQSKPISIKELSKQKKNLLKQATE
                     RGYPFASVFFKDIKIEQNQFHAKINAEKGPFITMDDITVEGEKIVNDNRIWHYVLDMK
                     KGSPIVADKIIHIDDRINELPFVSTTAPAQVIFKAEEASIKLFLKKKKSNHFDIILGL
                     QPASTSDPTQTKTILTGQITADLYNMIANGERIFFDYRRFNKDDQNVQLGLTWPYLPA
                     TRIGADANFQLQKRDTSHLDLLYNLGMKIPLRRRSSLMLIFQENISSILSINKAGIQS
                     SGKLPSVLDYKLSSFGIEGGITTLDYNISPTSGWDIRLKTLAGKRNIKKNQKIISLSR
                     PGADFSTLYDSIQSGTIQLRFDLFIDKYTTITRHQILRTGLQSGSILSGSHTLRNELY
                     RIGGYRLLRGFDEQSLEVSKYLVGTIEYRYLLSQLSYAFAFSDIAITNTRYGDTDFND
                     QPLSFGLGVTLQTKAGLFGIAAAVGQRKGLPFDFRSPKIHFGYVSVF"
     gene            complement(9939..13613)
                     /locus_tag="PLE23_03375"
     CDS             complement(9939..13613)
                     /locus_tag="PLE23_03375"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82339.1"
                     /translation="MKLYQNLLTSLIITALSFMANFTLHAQFMYDEVMKEGKPLNFYE
                     IQKKAEEYWATHDKKVKGNGYKAYKRWEYNWQNRVMDDGSFPPAGLVTENYNKYLQKY
                     RHQNYTDAGFRNNISWSSIGPASAISGYTGSGRINSIAFHPTNHDIFYAGSAGGGFWM
                     TTDGGTNWITTTDKIGRLGVSGIVVLPSDPKVIFIATGDGDAGDNKSVGVLKSTDGGY
                     TWNKTGLDWSAANDRLIRKLIIDPTDENIMYVAASNGIYRTVDGAKTWKIVITGNYYD
                     IEAKPQPGSNILYASTGGEIFRSSNNGQIWVKMQSIPGSNRIELAVTLADPTVVYAIS
                     SKSSTNGLNGVYKSTNSGSSFVLQSNSPNVLGYNSEGSEPGGQGWYDLILAADPADAN
                     TIYTGGVNCWKSTDGGLNWSLKSFWYQIPGVTNTHADKHSFAWRGNQLFEGNDGGLVF
                     TADGGDSWTNVSGNMTIGQIYRIGISQKDDKILAGHQDNGTKLGDVNGVWTNKFTGGD
                     GMECFVHPGNADILFTTSQNGHLNKSTNGGNNFYGITPVDYDGAWVTPFGVDPNEPNT
                     IYAAYKEVYKSEDAGENWTAISEDLSGDNLTLFRVAPSNSDYIYTGTSGRLFRTTDGG
                     TTWQELNTPGDRISMLTVNPADANTIYVVRQNYNAGQKVYKSTDGGDTWQNISSNLPN
                     IPANAILCTDLAPDALFVGMDVGIFFWSEQISDWVLLSENLPNVRISEFEFDKEKMML
                     YVATYGRGIWKTPIPLADAVCNFTSNIASFSDCDNFNGYTIGGKLTTQAKPRFSVADN
                     NPDNDAVLVNDAPLNASNCIKLNYNSKLKYNVGNSLQGRKILAPARLEWRMYIPEGKN
                     GSWTLQTDSLNYPLDVEYNGNGEAIVKSNGVQVSSFSFNPNTWMNISIIFNPAESKAE
                     LWTDKQFVYKYTSFTSGQITSLEFKGIASSPEDVFYADDLLYYEVKTEDCLTNGVTKF
                     VCVNGKVYKNSNYALCDAYTAKEWTEGTCCTLTGITTSAIDTKCGLDNGSATAQPIGG
                     KGISYTWSNGSKTATATNLSPGIYYVTVSSIEGCKSTDSVVVNASQGIMASVDSISIN
                     GLAVVVNDGLPPFKYIWNTGDTTSAIMPDTSGTYTVTVTDANGCSVVLEYIYIKVATK
                     DVKDIAVLIFPNPSTGIFNIKTDIILDRINVSDINGRILKSVELPANTIDLSELSSGV
                     YYLMLQTGNSVTYRKLMLLR"
     gene            complement(13843..>14761)
                     /locus_tag="PLE23_03380"
     CDS             complement(13843..>14761)
                     /locus_tag="PLE23_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82340.1"
                     /translation="IRKLILHITDKFPVSKSWEFLPQNTVFSNPYNPWLEPLVYEYNF
                     TNIKSDKTADFIGYKMGDVTNDANPDLLIGATETRTGQQYQLSTAAQDYKKGETVEMR
                     VQANKSESLKAMQFTMEYDVNALEFVGIEGDVPGIGEGNFGLRYLENGQITGCIEMKE
                     QYEGSSLFTVKFAAKADGKLQNDVRLSSARTPSLAFDESDVSMGLNLTFTGGAEPEKG
                     REVILYQNEPNPYTGSTLIRFSLPSEAEAVLSIYNAAGTLLKEVRGTYHAGENGVEIL
                     STDLPSQGIYFYQLTTGTYSGMKKMVFLK"
BASE COUNT         4345 a         3111 c         2856 g         4449 t
ORIGIN      
        1 taaaagcagc tgcttcatct aaccattaat ttgggctact tttgctgttc aaaataaggt
       61 attgatgaaa ataacattag cacagctcaa ctaccacatt ggcaattttg aaagcaacct
      121 tcgtaaaatg ctggacgcag taacgctcgc taaagaagaa ggcagcgaca tcatctgttt
      181 cggagagctc gccacttgcg gatacccacc acgcgacttt ctcgaattcc gggattttat
      241 tgccaggtgt atggattctg tccatgcgct tgctgctgct gcacagggca ttgccattgc
      301 ggttggttcg ccatgtgtca atcctgtagt ggaaggaaaa gatctgtaca acagtgcgtt
      361 ttttttattt gatggtaagg tacagcacat ttcgcacaaa gcattattgc caacctacga
      421 tatatttgac gaatacagat acttcgagcc ggcgcatgaa ttttcgaccg ttgagtacaa
      481 aggaaagcga atagcattta ccatctgcga agatatttgg aatgtaggaa acgacaatcc
      541 actctacaag gtatgtccga tggatgaaat gatgaaagac aaacccgata tcatgatcaa
      601 cctttctgca tctcctttca gttatgacca tgcggagtcg aggatttcgg ccgtaagggc
      661 aaatgtatcg aaatacggag tgcctatgtt ttatgtaaat catgtaggtg cgcagactga
      721 aatcattttt gacggaggtt cgcttgtagt atcgcctgac cttcaagtct ttgacgaaat
      781 gccctatttc gaagaatgta tcaaaaccta taacctggcg gatgtacaga aaggcggaat
      841 aaacaacgag cagcaaaagg aaaaatatag gcttatgcac gatgccattg ttacaggtat
      901 cagggattat ttcggcaagc tggggttaaa aaaagctatt cttggtcttt cgggcggaat
      961 cgattctgca ctcacagctg ttcttgcagt aagggcgctt ggcgccgaaa atgtgtgggg
     1021 tgtacttatg ccgtcagagt attcaaccgg gcattctgta accgatgcaa aagatctggc
     1081 cgtcaatcta ggcatcaggc atgacatagt gcctatccgg tcagtatttg atgtgtatga
     1141 aggcttgcta aatccattgt tcgaaggcaa acaggccgat gtgacggaag aaaacatcca
     1201 ggcacgcata cgagggatga tactgatggc attgtccaac aaatttggcc atatcctact
     1261 caacacatcc aacaagagtg aattcgccgt agggtatgga acactatatg gtgatatgtg
     1321 tggtggtctg gcagtattgg gtgatgtata taaaactgaa atttacaagc tggcagcata
     1381 cgtaaacaag gacggagaag tcattcctct aaactctata tgcaaaccac cgtcagcaga
     1441 attaagacca ggtcaaaagg attcggacag tctgccggaa tatgataccc ttgaccctgt
     1501 tctatttcaa tacattgaaa accgcatagg accggcagaa ctgatggaaa tgggcttcga
     1561 ccctgccatg gtgaacagaa tattaaggct tgtcaacatc aacgaattta agcgttttca
     1621 ggcaccaccg gtcatcaggg taagcaacaa ggcgttcggg gcaggacggc ggttgcctat
     1681 agtaggaaag taccttagct aatacacgaa tgatgctcaa cactaatatc gcccttgcaa
     1741 aatcaggaaa gtttttgtcg ttaaattttt ccttttcgtc agaataatta tcagattcgg
     1801 ttgtgataca gtaatttcac ataaaaaaat ttggaaactg tactcgaaat aaacaatctg
     1861 accaaagtat tcggcaaaat aacagctgtt gacagtctta gtttttcggt caataaaggc
     1921 gacatttacg gaattcttgg accgaatggc agtggcaaat caacaaccct cggtatggtg
     1981 ttgaatgtag tgaacccaac atcaggttca tggaaatggt ttggtgaaga accacagcaa
     2041 gaacatctca aacgcatcgg agccatcatc gaaagaccga atttctaccc ttacctcact
     2101 gctacacaaa atctgaagat agtagcaaag ataaaaaatg caaacccaaa taacatcgaa
     2161 cacaaactca gaacagtggg tctgttcgac cgcaaagact cggtatttag cacttactcg
     2221 ctgggcatga aacaacggct ggctatagca gcagccctgc tcaacgaccc agaaatactc
     2281 cttcttgacg aacctaccaa cggactcgac ccgcaaggca ttcgcgatat cagagacatc
     2341 atccgacaga tagccacagg cggtacaact atattgctcg ccagtcattt gcttgatgaa
     2401 gtcgaaaagg tctgcagcca tgtagtggtt atcaagaatg gtaaaaaact gtacagtggc
     2461 aaggtagatg aaatgacggc ctcatacggc tacatcgaag taaatgcaga taacagaaca
     2521 ttgctgcttc aaaaactcgg tgagttgggc tttacaaaaa ttcagcagga aaaagattgc
     2581 atcaaactga tacttgaatc agaaaccgat acccgctcac tcaacgaaac ccttttcaaa
     2641 agcggtgtca cactcacaca cttggtgaag agaaaaaata cgctggaaga acaatttatc
     2701 gaattaacag gacaaaaaat gcatgcatga aaaatctgat ccaactcgaa ctactcaaac
     2761 tctggccaaa ccgcagtttc aggatatata ccctacttta cttctgcatt attgttggct
     2821 tgtttttcct gtctgttatt gaattcaatc tcggtccatt aaaatttagt tttggcgcac
     2881 agggtgcatt caaatttcca tacacatggc atttcaacac ttatatagca agtcatttta
     2941 agattctgct ggcactcatc atcattacca acattgtaaa cgaatatgat ttcaggacta
     3001 tcaaacagaa tttcatcgat ggtcttagta aaaaagaatt tgtcacatcc aaagtactca
     3061 cttttgtcat cctcagttta tgttctacta tcttggttgg aatgattgca ttggtacttg
     3121 gactcaaatt taccggcaac attacagcag gcatggtttt tcgtgaaatg tattacctgc
     3181 ctatttattt ccttgaatta atcgccacct ttagtttttt gatgtttgcg gccgtactaa
     3241 tccgcaaatc ggcctttgca cttggttttt ggatattttg gtcaactgcc gaatcaatat
     3301 tgcgcaatgt ctggaaatca aaaataccaa atacttttaa tccgttcgat tttttcccga
     3361 tgcaaagcat cagtaatctt attcacgaac ctttttcgag aatggaaacc gtgcagaaga
     3421 tttcaaatat ggtacaaccc ggcaaagtaa tagattattc gattcaatgg ccttatgtct
     3481 taatgtgctt atgttggtct gggttgtttg tgtatctgag ctattacttg atcaaaaaac
     3541 gtgatttgta agcacatata tcaggtaaaa taattaccat aaatctgttg cggcttttat
     3601 ttcaacaaca acgaaacata tttttttcaa aaaagtatac ttaattaaaa aatcaattgg
     3661 atatgatgtt gccatagccc aattgacatt aaattcctct attttaaaaa agattaaaca
     3721 agattaaaag atgggctacc tagaaaataa tttattgggg ggtgaatcaa tttattatca
     3781 gacccatgta cattggacaa tttatataaa agggttgtta agtttaattt ttggtatttt
     3841 cttaatcagt atagaagttg attctttggg tataatcttc atattaattt catttcttat
     3901 attaagcatg tctaaattaa agattcataa ctcagaaata gcagtaacaa ataaacgcgt
     3961 gttgatcaaa gaggggttga ttacaaccca atccttagaa ttaatgctga ataaaataga
     4021 agcaatacac gttaagcaag gattaatcgg taaaatgttc aatagtggta ctatagaaat
     4081 aataggtact ggtgggacaa attcccattt ctcgaacatt tttaaccctt tggagtttag
     4141 acgtgccgta aattaccaaa taactaaatc aaatctatag tctatattta aaacaagctt
     4201 attgtttatc taattaaatc ctagctatac tcagtataag aattaagtaa ccacatacaa
     4261 caccgacgca atccggtcaa cgaatatttt gccggaacgc gtgagcaaac aatgatctcc
     4321 atctacaatc atctggccgg caaaaatatg ttcctgactt catcaatgcg ccacccaaaa
     4381 ttagtttttc caaaaagcag gttgaaaaag cttttctctt aatttttatt tttctgttaa
     4441 taacattgtt ttataaatca atttacctgc tttgttcagt ctaatttcta aggttactat
     4501 agtttttgct ataaatagca ctgtatcgac acttagtgga atttgattaa ggggtcaata
     4561 tcgccggaat agggggtcag tatcacccgg aatatccaaa aaaaccaggc tctctgaact
     4621 catcactctt ttctgtttca aaatataaag tagttacatc ataaaacagc agccctattt
     4681 tgcctccaaa tagttctttt gtgtgtttta cactaatttg ttgaattctt tcttgttggc
     4741 tgttgtataa tttgtccata taacggtata tcttattcaa atctgaatct tctccaaatt
     4801 aggatcttag atactctacc gttgcatttt tgcttaatgg ttgagccaat ctagctgtta
     4861 caagacttct taattcttta tcctcaatgg aattaaaccc aatagaatca tatgcatagt
     4921 taactataag ttgaatccca ttaagataga tgttcgctat actatctaat aaatgaatgg
     4981 caatttgttt ttcctctttc tcttgttcat gtttttcaaa aatatcctca ccatttgaat
     5041 attggtctat ccactttcgt ccttttgcaa ccaaccgatt tatttctgac tcatcactac
     5101 ttaccccaat agttctaagc tcccgatatt taccagagct tttatcaaca attacaacac
     5161 taaatgtccc ggatttattc cttttctttc gtacaaacat attcaaattt agtgttttta
     5221 gcctttgcgc cacccaaatc acctcgcaaa aatttgcaat gtattgaaat ttagtgtatt
     5281 atgaaattga acctgaaaaa gtgatgaagt caggagatta ttcgattcaa tggccttatg
     5341 tcttaatgtg cttatgttgg tctgggttgt ttgtgtatct gagctattac ttgatcaaaa
     5401 aacgtgattt gtaagcacat atatcagggc atacattttg gtttccggga attgcgatag
     5461 ccaaattata attaagataa aaaagcggta aagattcgaa aaaaatgagt ctttaccgct
     5521 ttttggttta ccgcttacat attcctgtca gaatagtgtt tattccggca tggaaagttt
     5581 agtggctgac tataaaactc tccgttataa tagcctgatt aagcccatct agaatactaa
     5641 cgatgtattg tccactggta agattggcag aaagatcaaa gctgagcaac tctccatttg
     5701 cttgtacctt gcctgactga acaatcctgc caagaaagtc ggtaatggtg taggtgtttt
     5761 gtgaaacatc caaacctttt accatcattt tcagttgcgt acccttcaac ggatttggga
     5821 ataacacagt cgtaaatgct tgttggttta cattttcatc agacaacaac aagtcatccg
     5881 tattatagaa aaatatagta ggtgtagtga atgtatcggc tgcataattc acgcctgcaa
     5941 gcggaacata gctgcctttt ctgtagaaaa tatatctgtt gtccactcct gtcacctgac
     6001 cctgtgtgat gccaaatgcg ttaagcaaaa ccgccggagc cggctgtccg aaaaggaaga
     6061 aactatcaat tgcaaactga cttgactgat cgacgagtac gtcatagtat ttgcttgggc
     6121 cattaggtgt atgcaggcgc attttgccat aaccgacgat ggtatccagt cttacaaggt
     6181 aataactctg aacaccgggt gctttattaa gggctgctgc agttactgtc aggttaaaat
     6241 tcgttttccg atagctcaca gattgccagc tactgttatc cgtaaaaggg aattgaatga
     6301 ttttccttgg tgagccccat tgccaacctt gtaaaggtat tgtcaggctg tctttgttgt
     6361 ttcctgtcaa agcacccaga ccgtacgcct ggttgtctat atacatcatt ttgtccagta
     6421 cagccgtatc attgaaatca atttcataga atacgttata accaagggtc tgtgtcaatg
     6481 ccttaaatcc actttgataa acatccactc cttcatcagt aaatactggc agtgtctcct
     6541 tataatatgc aagtaccaat gtatcttcac cttcggtctg gctatagtcc caagtgccgt
     6601 ttttctgtgg aacaggagca ggcgccaagt tgagcaggtt tttgaagttg tacaaaccct
     6661 caggcacagg catgtcggag gcatttattg ttatcggtgt ctgggagtgt aataagctcc
     6721 caataaacat cagaaagcaa atggtaaata ttcttttcat gacattaagg ttttagaaat
     6781 taaaaaagct gtttatcacg ataaagttaa aaaacaaaat tcagaaataa ataactttaa
     6841 cataattttt atacatctct tcctgtagtg caataattat attcctaaca ttatgtaagc
     6901 acaaaaaggt ctgatgctat accgtctgca agtatcttcc cttcttgggt taaaatacat
     6961 gaacctgact ctagcttcat ctggccggca gaaatatatt tttgtatgtt ttgttgaaat
     7021 aacgtagcgt acgacgaatc caattgttcc aatgcttgta aactcacgcc ccacatcgtt
     7081 ctcaaaccgg tcatgattag ttcgttgaat cggtctgcgg tagtcagcaa ctctgattcg
     7141 gcaggtatga taccattcac tacagatttg atatattgtc tgttgttggc aatattccag
     7201 catctgcttc gcccatcata ggaatgtgcc gaaggaccaa taccaagata atggacaccc
     7261 tgccaataag atgtgttgtg caaggcgtat tgcctgtttt tggcaaaatt ggatatttcg
     7321 tattgttcga agccggcttc tgtgagcgtt ttcaccatca ttacaaactg tgtagcagat
     7381 ttatcagcat tgacagtctg tgcctgacct gtcctgatca tcttactgag tggtgtcttg
     7441 tcttctaccg taagggcata agccgaaata tgttctatcc ccaattctat tgccctttgc
     7501 atgttatgct gccacatagc atctgtggtg gttggggcac catatatcag gtcgatggta
     7561 agatgattga agccagtgtc cattgccatt ctgatacaat tttcggcttg ttgccgggta
     7621 tgtgcccgat gcatccagcg caaatcttct tcaaaaaacg actgaacacc tatgcttaat
     7681 cgatttacgg gtgtttgact tagtgcctca agataaccgg ctgtaagatc gtcaggattt
     7741 gcctcaaaag ttacttcacg aagatccgtg tgataatgtt tgaataccgc atcaaatatc
     7801 gacatcgttt cggcctgtgt aagcaatgat ggtgtaccgc caccgaagta aatggtttct
     7861 atcgtggcat caggtccaag gtatgccttg cgcagttcaa gttcgatgag caatgcctgt
     7921 acaagctcat ttttatatct gtggctggtg gcaaaatgga aattgcaata atggcaggcc
     7981 tgcttacaga atggtatgtg tatatatatt cctgccataa aatatccagt agtttgataa
     8041 tttttggtca aatttacgtt ttattcatct atggtggctt atagtatcga acatacgcat
     8101 catttaccga ccaataggct cctgtcaata ctatttacag gactgttgtt gcttgccggc
     8161 aatcatattt ctgctcaaaa gaggtatatt ctggagctac agtatgactc gggacaaaaa
     8221 accatgacca gactatttca ggattcgctt tcagccgaat tatttataca atcagagata
     8281 aaggcattga tgtcaaaagc gcatctggag gctgctgttg atcaaaaaaa atatccaaat
     8341 gattcgacaa ctgttgcaac catccatatt ggtccggagt acgaatgggc tgaactggca
     8401 tggtcggtaa ctgacccgat attccgaaaa ccggctaatt gggacaaaaa gtggcagtca
     8461 aaaccaattt caataaaaga attatccaaa caaaaaaaga atctgctcaa acaagctacc
     8521 gagcgcggat atccgtttgc ctcagtgttt tttaaggata taaaaattga acaaaaccaa
     8581 tttcatgcaa aaattaatgc agaaaaaggt cctttcatca ctatggacga catcacggtg
     8641 gaaggtgaaa aaatagtcaa cgacaaccgt atctggcatt atgttctgga tatgaaaaaa
     8701 ggaagcccca tcgtagcaga taaaatcatt cacatagacg accgcatcaa cgaactgccg
     8761 tttgtaagca ccacagctcc tgcacaggtc atattcaagg cagaagaggc aagcataaaa
     8821 ttgttcctga aaaagaaaaa atcaaaccat tttgacatca ttcttgggct acagcctgct
     8881 tctacgtccg accctacaca gaccaaaacc atactcaccg gtcagataac tgctgatctg
     8941 tacaacatga tagccaatgg cgaacggata ttttttgact atagaaggtt taacaaagat
     9001 gaccagaatg ttcagttagg gctaacatgg ccttatctgc ccgctacacg aataggcgct
     9061 gatgctaact tccagctaca aaaacgcgac acatcacacc ttgacttgct gtacaacttg
     9121 ggcatgaaga taccactacg cagacgttca tcattgatgt tgatatttca ggaaaatatt
     9181 tcaagtattt tatccataaa taaggctggc atacagagtt cgggcaaact cccttcggtg
     9241 cttgattaca aactgtcatc attcggtatt gaaggtggca tcactacgct tgactacaac
     9301 ataagtccta ctagtggctg ggacatcaga ttaaagacat tggccggtaa gcgaaacatc
     9361 aaaaagaacc agaaaatcat ctctctgagt cgtccgggtg ctgatttcag tacactttat
     9421 gacagcatac agtccggaac catacagctt aggttcgatt tatttatcga taaatatact
     9481 accataacca gacatcaaat cttacgtacc ggattgcaaa gtggcagtat cctgtcgggt
     9541 agccacacac ttcgcaacga actctaccgg ataggtggat accgactgct tcgcggtttt
     9601 gacgaacaat cgcttgaagt gtcaaaatac ttggttggca ctattgagta caggtatctt
     9661 ctgagccaac tttcctatgc atttgcattt tctgacatag ccataaccaa tacacgatac
     9721 ggagatacag acttcaatga ccaaccactt tcctttgggc ttggcgtcac attacagacc
     9781 aaagccggac tcttcgggat agcagcggca gttggacaaa gaaaagggct cccgttcgac
     9841 ttcaggtcac ctaagattca tttcggctat gtaagtgttt tttgacttct ttggtctgta
     9901 aaaatatacc tgaaatgttc acacttttca tattacaatt acctcaaaag cattagcttt
     9961 ctataagtaa cagaatttcc tgtctgcaac ataagataat aaacaccgga gctcaattca
    10021 gaaagatcaa tcgtgttcgc cggcaattca acagacttta atatcctgcc attgatatcg
    10081 gacacattga ttctgtcaag gatgatatct gtctttatat tgaatattcc ggtagaaggg
    10141 tttggaaata tcaaaaccgc aatatctttg acatctttgg ttgctacttt tatataaata
    10201 tattctagca cgacactaca gccatttgcg tctgtcacag ttactgtgta agtcccgctg
    10261 gtatcaggca tgatggcaga agttgtgtca ccggtattcc agatatattt gaaaggaggc
    10321 aaaccgtcat ttactactac tgcaaggcca ttgatactga tactgtcaac agatgccata
    10381 ataccttgtg atgcattgac cacaacagaa tcagtggatt tacaaccttc tatggaagac
    10441 actgtaacgt agtaaatacc cggactaaga ttggtggcag tagctgtttt agagccatta
    10501 ctccatgtat aactaatacc ttttcctcct attggctgag cagttgcaga gccattatcg
    10561 agcccacatt tagtatctat agctgaagta gttatgccgg tgagggtaca acacgtacct
    10621 tctgtccatt cttttgctgt ataagcgtca caaagtgcat aattcgaatt tttatacact
    10681 ttaccattca cacacacaaa cttggtaaca ccattggtaa ggcaatcttc tgtttttact
    10741 tcataataaa gcaaatcatc tgcataaaat acatcttccg gagatgaagc gattccttta
    10801 aactcaagag aagtaatctg gcctgacgta aatgacgtat atttatatac aaactgtttg
    10861 tctgtccaca attcagcttt tgactctgca ggattgaaaa ttattgaaat attcatccag
    10921 gtatttgggt tgaatgagaa ggaagaaacc tgcacaccat tgctttttac gattgcttca
    10981 ccattcccat tatattctac atccaatgga taattaagtg aatctgtctg aagtgtccaa
    11041 cttccatttt ttccttccgg aatgtacatc ctccattcca ggcgcgcagg ggccaagatt
    11101 tttcgacctt gaagactgtt gcctacattg tattttagtt ttgagttgta attgagttta
    11161 atacaattgc ttgcattcaa tggggcatcg ttaactagta cagcatcatt gtcagggtta
    11221 ttgtctgcaa cagagaaacg tggtttggct tgtgtggtta attttccacc gattgtataa
    11281 ccattgaaat tatcacaatc gctgaacgaa gcaatgttgg aagtaaaatt gcaaacagca
    11341 tctgccaaag gtatcggggt tttccatatt ccacgaccat aggtggctac gtatagcatc
    11401 atcttttctt tgtcaaactc aaattcactt attcttacat ttgggagatt ctcactcaac
    11461 aaaacccagt cagatatctg ttcggaccag aaaaatatac cgacatccat tcctacaaac
    11521 aaggcatctg gcgccaggtc ggtacaaagt attgcattgg ccggaatgtt cggcaggttg
    11581 cttgaaatat tctgccatgt atcaccaccg tccgtggatt tatatacctt ttgaccggca
    11641 ttataattct gccttaccac ataaatggta ttggcatcag caggattaac tgttaacata
    11701 ctgatcctgt cacctggtgt attcaattcc tgccatgttg tacctccatc tgttgttctg
    11761 aagagtctgc ctgaagtacc tgtatagata tagtctgaat tggaaggagc aaccctaaaa
    11821 agcgtaaggt tatctccgga taggtcttct gatattgcag tccagttttc tccggcatct
    11881 tccgacttgt aaacctcctt gtatgcggca taaatagtat tgggctcatt aggatcaaca
    11941 ccgaacggag taacccatgc cccgtcataa tcgacaggcg taatgccata gaaattatta
    12001 ccaccgttgg ttgatttatt caaatgcccg ttttgggatg ttgtaaacag tatatcggca
    12061 ttccccggat ggacaaaaca ttccatacca tcacctccgg tgaacttatt tgtccacacg
    12121 ccattcacgt caccgagctt ggtaccgttg tcctggtggc cggcaaggat tttatcatcc
    12181 ttttgtgaaa tgccaattct gtatatttgc ccaatggtca tattgcctga tacatttgtc
    12241 cagctatcgc cgccatcagc tgtaaagaca agtccgccat catttccttc aaagagttgg
    12301 tttcctcgcc atgcgaatga atgtttatcg gcatgcgtat tggtcacacc cggtatctga
    12361 taccaaaatg atttaagtga ccagttaagt ccgccgtcgg tagatttcca acagttgaca
    12421 cctccggtat aaatagtatt ggcatctgcc ggatctgctg caagtatcag gtcgtaccag
    12481 ccttgtccac ccggctccga gccttcagaa ttatagccaa gtacattcgg cgaatttgac
    12541 tgaagcacaa aggaagatcc actgttggtt gatttataaa caccattcaa tccattggtg
    12601 cttgattttg aactaatggc atataccact gtagggtcag caagtgtcac tgcgagttct
    12661 atacgattgc tacctggtat tgattgcatc tttacccata tctggccatt attgcttgat
    12721 ctgaatattt caccaccggt tgatgcgtac aagatattgc tgccaggctg aggctttgcc
    12781 tctatatcgt aataattacc cgtaatcact attttccatg tctttgcccc gtcaacggtt
    12841 ctgtatattc cgttacttgc tgctacatac atgatgtttt catctgttgg gtcaatgatc
    12901 agttttctga tcaatctgtc attggctgct gaccaatcga gcccggtctt gttccaggtg
    12961 tatccaccat cggtagattt tagcacaccg accgatttgt tgtcgcctgc gtccccatca
    13021 cctgtagcaa taaaaatgac tttagggtca gaaggcagta caactatgcc cgacactcca
    13081 agcctgccaa ttttatctgt tgtggtaatc cagtttgtac caccatcagt tgtcatccaa
    13141 aacccacctc ctgcagaccc ggcgtagaaa atgtcatgat ttgtaggatg aaaggctata
    13201 gaattaatcc tgccactacc tgtatagcca cttatggcgc tggcagggcc aatggatgac
    13261 caggaaatat tattgcggaa tccggcatct gtataattct ggtgtctgta tttctgaagg
    13321 tatttattgt aattttctgt caccaatccg gccggtggaa aactcccgtc gtccatcaca
    13381 cggttttgcc agttatactc ccatcttttg taagccttat aaccattacc ttttaccttt
    13441 ttatcatgag tagcccaata ttcttcagct tttttctgaa tttcgtaaaa gttaagtggc
    13501 ttgccttctt tcattacttc atcatacata aactgtgcat gcagcgtaaa attcgccata
    13561 aatgacaatg cagtgatgat cagagaggta agaagatttt gatataattt catgcttgaa
    13621 tattttcact tacatcaaca accaaagctg aagaattgtt gacgcaactg tacatttata
    13681 aaaggtgcca aaaatactaa tcatcagcat aaaatgtaca gtttgtcaat attttaactc
    13741 cattaaagac aattattgcc acccaatact gaatttatta aaaaaagaca cctactccga
    13801 tatacgaaga aggtgtcttt tgctgttatg gcctcatctc ttctacttca ggaataccat
    13861 tttcttcatt cctgaatacg tgccggttgt aagctggtag aagtaaattc cctgagatgg
    13921 aaggtcggta ctaagtattt cgactccatt ttcgcctgca tggtatgtac ctcgcacttc
    13981 cttcaataac gtaccagcag cattgtatat actcagcact gcttcagcct ctgaaggcag
    14041 actgaatcgt atcaatgtgc taccggtgta aggattcggc tcgttttggt ataaaatgac
    14101 ttctcttccc ttttctggct cagcacctcc ggtgaatgta aggtttaatc ccatactgac
    14161 gtcagattcg tcaaatgcaa gagatggtgt acgtgccgaa ctaagtctca catcgttctg
    14221 tagctttccg tcagccttcg ctgcaaattt taccgtaaac aacgatgatc cttcgtattg
    14281 ctccttcatt tcgatacatc ctgtaatttg tccgttttca agataccgca gaccaaaatt
    14341 accttcacct atacctggta catctccctc tattcctaca aactcaagtg catttacatc
    14401 atattccatc gtaaactgca tagcttttaa tgattcggat ttattcgctt gtacccgcat
    14461 ttcgacagtt tcaccttttt tatagtcctg tgcggctgta ctcagctggt attgctgccc
    14521 ggtacgtgtc tcggtagcac ctatcaacag gtcaggattt gcatcattgg tcacgtcgcc
    14581 catcttatat ccgatgaagt cagcagtttt gtccgatttg atgtttgtaa aattatactc
    14641 atacacgagt ggttcgagcc atgggttgta tggattgctg aacaccgtat tctgtggtaa
    14701 aaattcccaa gacttcgata ccgggaattt gtcggtaatg tggagtatga gtttacgtat
    14761 t
//
LOCUS       DAOQQY010000035        14664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1332_length_14664_cov_5.741118, whole genome shotgun sequence.
ACCESSION   DAOQQY010000035 DAOQQY010000000
VERSION     DAOQQY010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14664
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1332_length_14664_cov_5.741118"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="PLE23_03385"
     CDS             <1..732
                     /locus_tag="PLE23_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82341.1"
                     /translation="VVPGGTGSVVLEFDNVANGSTLSLGKDYTNANGDVMNFSIFDYY
                     VSNFSLVKEDGSVYIVPKDSCYFLIREKGGSNTEIELKNIPAGNYKEIRFLVGVDSLK
                     SVSPIADRTGVLDPAGEGADMYWAWNSGYIFVKAEGTSPQAPLDAASNTNKFRYHIGL
                     FGGYTSATLNNIKSFALHDEHGEVAEVRTNKKPHYHIIVDVMEMFKNPVTVSVTENHT
                     VMVSPYSKVIADNYADMFKLGHIHN"
     gene            797..1825
                     /locus_tag="PLE23_03390"
     CDS             797..1825
                     /locus_tag="PLE23_03390"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMX82342.1"
                     /translation="MKNLTYLLVAIIFFSTIGCMKTDEGVPFTGLQLPSHFPAPVHSM
                     ADNTVTKEGFELGRKLFYDPILSRDNTISCGSCHVQGSAFTHHGHDVSHGIDDQVGKR
                     NALPVQNLLWQNTFFWDGGVHNIELISLNPIKNPVEMDENFPRALEKLRTHPEYPSLF
                     KQAFGSAEINSERTLKAMTQFMAMLISADSRYDRYKKGTVQFTESEKQGYELFVQKCA
                     GCHKGELFSDFTYRNNGLTSDFSSDKGRYEISLLDDDIGKFKVPSLRNIAKTKPYMHT
                     GSFYTLQDVLDHYSDGVKMSPTLDPLLQTGTKPGIEMTKEEKSKIIDFLNMLTDEGFL
                     KNPMFAEQ"
     gene            complement(1935..3206)
                     /locus_tag="PLE23_03395"
     CDS             complement(1935..3206)
                     /locus_tag="PLE23_03395"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMX82343.1"
                     /translation="MEIVIIGILILINGWLVLAEMSLVSSRKARLEAAAKKGGRKAQT
                     ALELHENPKRFLPTVQIGITLIGILTGVFSGKSITEAIHTWLLKVELLAPFAETLSVV
                     IVVFIITYFSIIFGELLPKHIALMYSDGIAKSIAIPMNFISRITYPFSWLLVKSLDLV
                     IFLTGLRKSDESKVTEEEIKAIIQEGREDGEVQEIEQEIVERVFLLGDRRVSTLMTPR
                     SQVVMLQGNHTMKEALPIMSEEIHSIYPVYEKDKDNVIGVARLKDIFSNIKNDKLLIK
                     SLASEANFILENTSAFQALEKFKETHVHYGIIIDEYGQMQGIITLNDLLESMVGYTSD
                     QYNDSYTIAEREDGTWLIDGHYPFHDFLHYFDLEQIDPEVNFDTFGGLILNEMGKIPS
                     QGDKIVWDKFEFEIIDMDGTRIDKILIRKTE"
     gene            complement(3384..3467)
                     /locus_tag="PLE23_03400"
     tRNA            complement(3384..3467)
                     /locus_tag="PLE23_03400"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3431..3433),aa:Leu,seq:caa)
     gene            complement(3468..3992)
                     /gene="hscB"
                     /locus_tag="PLE23_03405"
     CDS             complement(3468..3992)
                     /gene="hscB"
                     /locus_tag="PLE23_03405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HMX82344.1"
                     /translation="MNYFELFEIPVSFFPDREDIKRRYHQLSFKYHPDFHTIDSEYDE
                     SEILEKSAEINQAYQSLTDEDKCLAYILKMCDALPEEGKATVPQDFLMDMMDINEEVM
                     DLQLDPDEEKLYSINTKIKNLESELFDEIYPVMQSFDFQIQNDSALKKITDFYLKKKY
                     LLRLKQNLLNFAQP"
     gene            complement(3999..5324)
                     /gene="rseP"
                     /locus_tag="PLE23_03410"
     CDS             complement(3999..5324)
                     /gene="rseP"
                     /locus_tag="PLE23_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HMX82345.1"
                     /translation="MITIGQLMLSLMILVVLHEMGHFLPAKWFKTRVEKFYLFFDPWF
                     SLVKKKIGDTEYGIGWLPLGGYVKISGMVDESMDTEQLKQPPQPWEFRSKPAWQRLII
                     MLGGVTVNFILGFFLYAMVLFVWGEKYLPATEVKNGIYVDSLGTQLGLMDGDKVLKVG
                     DIPFDKFNDRILVKEIIINNINKMTIERNGHEMVIDISPDKAAMLATAKNSRAELFGA
                     RMPFVVADVVKAGPADGAGIKKEDKIIGINGTPVEYYHEFKRVINKLPNQAISLNVLR
                     GNDTLNVKLTTSDKKTIGVAPYPPDHYFKFETQKYSFASAIPAGINKGLSFLGDQFKA
                     FGQMFKGKIKASESLGGFGSIGSMFGDVWIWERFWTMTAILSLILAVMNLLPIPALDG
                     GYVVFLIYEIVSGRKPSDKFVEKAVTVGFFILLALMVYANGLDIVRKFF"
     gene            5432..6136
                     /locus_tag="PLE23_03415"
     CDS             5432..6136
                     /locus_tag="PLE23_03415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HMX82346.1"
                     /translation="MQLFYTTHQEGDFLILEGEEAHHCANVLRHQAGHQINVTNGKGS
                     LFHCTIIDKTKTTVRLMPDSEQKFETPAHLNLSIAIAPTKNIDRFEWFVEKATEIGIG
                     RIIPLHTKRSERTIIKHERLFKLMISAMKQSNHFYLPELHPLTTFKELITGVKTDIAL
                     IAHCMADELPHVSKFIKPDRDVLICIGPEGDFTAEEVHTSENAGFTGVSLGKSRLRTE
                     TAGIYACISFHQALIM"
     gene            6192..6851
                     /locus_tag="PLE23_03420"
     CDS             6192..6851
                     /locus_tag="PLE23_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HMX82347.1"
                     /translation="MKKLFISIFFLFSFLAISSAQKSSLKLALLKYSGGGDWYSVVDA
                     LQNLVTFTNQNLNTSFQVDYATVDVGSAEIFNYPLLFLTGHGNIILSDQEAENLRNYA
                     LAGGFILIDDDYGIDPYIRPVIKKIFPQAELTEIPFNHPIYHQKYDFPKGLPKIHKHN
                     DKPTQGFGIFIEGRLVCFYSFESNISDGWESFEVHKDDQAIRLAALKMGANIIQYAFM
                     Q"
     gene            complement(6930..7664)
                     /locus_tag="PLE23_03425"
     CDS             complement(6930..7664)
                     /locus_tag="PLE23_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82348.1"
                     /translation="MSNNILFIFEGQKTEKLISDSLFRYFLNENTVVTCAYCTTIYKI
                     HNEFLEDEYLDTFNLVKDLDANKEILKDFKRTDFAQIFMFFDYDGHASNAGDHKLINL
                     LNFFEEETEKGKLYISYPMVEALKHVEDFNTFHILAVACKNFSVYKNTVNKKCLKELK
                     HFNLYNENIWEKVISTHLYKMNQIVNNTYTFPTTIINQLTIFNKQLENHKTLNKTIAI
                     LSSFPVFLHDYYGNQELKHRLNEFPH"
     gene            complement(7654..8490)
                     /locus_tag="PLE23_03430"
                     /pseudo
     CDS             complement(7654..8490)
                     /locus_tag="PLE23_03430"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(8542..8772)
                     /locus_tag="PLE23_03435"
     CDS             complement(8542..8772)
                     /locus_tag="PLE23_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82349.1"
                     /translation="MLRKFKVSNFKSFENDILLDLTAASGYEFNKSSIKKGLINNAII
                     YGRNGVGKSNLAFAIFDIIEHLTDKERNEHLY"
     gene            complement(9002..9217)
                     /locus_tag="PLE23_03440"
     CDS             complement(9002..9217)
                     /locus_tag="PLE23_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC family transcriptional regulator"
                     /protein_id="HMX82350.1"
                     /translation="MENPLKDLVERSAFGVCSYLGDKIGISHTRVRMYFCYISFLTVG
                     SPVVIYLFLAFWVNIRNYLRKSSTIWS"
     gene            10255..11661
                     /locus_tag="PLE23_03445"
     CDS             10255..11661
                     /locus_tag="PLE23_03445"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82351.1"
                     /translation="MKNNSLNSKLNLTKLVNELTIMSQNVKVDSVEETAFTQIISRFH
                     QDDNIEQAIAGFEEAIYKNRLTIAFMKKKISQLIENKIADLAIETISLATQLAPHDRE
                     LHFLMIQACRLNEDYSRALDILDTLKADATPREVILILNEEAAIYKLMQNAESTFVSL
                     RRLLTIDPHNEDALREIWWSIEMSKNYKESLKLHKIVIEKNPYSARAWYNLAHAEYYL
                     HQYENAIRSFEYAYIIDPKFELAYRYCAEVCTLTGRFEQALKCYYDLMEHAVADAEAL
                     KSIGYCYEKTSNLIKARKYYLLARNLDPVDDEIYYNLGNTYKADGQWQLAANYYERAV
                     SLQDRNEDYFVALAEAYIHCGENHKADLFFRRATEVCPEMPAPWVRYAEFYFKGGKSE
                     KALEILLEAEETTAGAEIYYCRAIILFSLNEKFDAMENLGEALQIDFDMHKILFDYLP
                     ALKEDREIKAIIKYYEFG"
     gene            11823..12698
                     /locus_tag="PLE23_03450"
     CDS             11823..12698
                     /locus_tag="PLE23_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX82352.1"
                     /translation="MKVAGFTFIKNAVKFDFPIKEAILSILPICDEVVVAVGDCEDGT
                     LGLIQSIGSDKIRIIRTVWNPELTTGGKVLADETNKALAAVSPDADWCFYIQGDEVFH
                     EDGIEKVQQAMLQYKDDSTVDGLLVKYLHFYGSYDYIATSRHWYRNEIRIIKNSRSIF
                     SYKDAQGFRKNNDEKLYVVPVDAFMYHYGWVKEPKIMHKKMQNADTIWHSAKDNEARQ
                     IPVVAEFDYSGIDMLATFVGKHPIVMKDRISSKNWHFEYDLSYNSMSLKEKIKDLVYR
                     LTGRELFSYKNYRLK"
     gene            12986..13420
                     /locus_tag="PLE23_03455"
     CDS             12986..13420
                     /locus_tag="PLE23_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMX82353.1"
                     /translation="MFSKSCEYGIKAIIYIATQSLEDVRVKIGDVAENTGTPEAFTAK
                     ILGSLTKHNIVSSIKGPRGGFEIDKSRMKNIMVSEIVAALDGDAIYNGCALGLSNCSD
                     VDPCPLHDSIVEVRNKLRKNLESTTVYDLATKLKSGKSILIR"
     gene            13540..13776
                     /locus_tag="PLE23_03460"
     CDS             13540..13776
                     /locus_tag="PLE23_03460"
                     /inference="COORDINATES: protein motif:HMM:NF022969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexameric tyrosine-coordinated heme protein"
                     /protein_id="HMX82354.1"
                     /translation="MEKKEIWLESLITETPQEGRELAIKMSRKTIAAIQTDPEVRKKL
                     RPDYANDTAQLIASANVVAIEFQTIAQANNYWRK"
     gene            13886..14281
                     /locus_tag="PLE23_03465"
     CDS             13886..14281
                     /locus_tag="PLE23_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HMX82355.1"
                     /translation="MTETKEIENLEDIQLMVDSFYGRIRQDEMLGPVFNNVIQDKWPA
                     HLEKMYRFWETVLLDKHSYYGAPFVPHAKLPVQKEHFDRWIDLFSATVDTFFHGEKAE
                     RAKWQGNRMAEMFLMKIQYHRNSSSIPLI"
     gene            14344..>14664
                     /locus_tag="PLE23_03470"
     CDS             14344..>14664
                     /locus_tag="PLE23_03470"
                     /inference="COORDINATES: protein motif:HMM:NF016303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HMX82356.1"
                     /translation="MDITKESIIGELVANDYRTASVFKKNNIDFCCQGNRTIEVACKE
                     KQLDTDKLVKELANAATQIQSGSVDYKSWPLDLLADYIEKTHHRYVTQKSEEIMPFLD
                     KIIRV"
BASE COUNT         4674 a         2729 c         2867 g         4394 t
ORIGIN      
        1 gtcgtaccgg gtggtacagg ctcggttgtc ctggagtttg acaacgtggc taatggaagc
       61 acgctcagtc ttggcaagga ttatacaaat gccaacggag atgtgatgaa tttctccatc
      121 tttgattatt atgtaagcaa tttttcgctc gtgaaggagg atggttcggt gtatatagta
      181 ccaaaagaca gctgctattt tcttatcaga gaaaagggtg gatccaatac agaaatcgaa
      241 ttgaagaaca ttccggcagg aaactacaag gaaatcaggt ttttagtagg tgttgacagt
      301 cttaaaagtg tgtcaccgat agcagacaga accggtgtac ttgatccggc cggcgaagga
      361 gccgatatgt actgggcatg gaacagcggt tacatcttcg ttaaggctga aggtacctca
      421 ccacaggctc cgctagatgc tgccagcaat accaacaaat tcagatatca catcggcctg
      481 ttcggcggat atacttctgc taccttgaat aatatcaagt catttgcact ccatgacgag
      541 cacggtgaag tagccgaagt acgaacgaat aaaaaaccac attatcacat aattgtcgat
      601 gtgatggaaa tgttcaagaa tcctgttacc gtaagtgtta ctgaaaatca tactgtcatg
      661 gtttcacctt actccaaggt gatagcagac aattatgctg atatgttcaa gctcgggcac
      721 attcacaatt agataaaaag tcatcaataa agtcaaaaat tcgtttttgc tttttgaaat
      781 ttttaaaata aaagagatga agaacttaac ataccttctt gtagcaatta tttttttttc
      841 tacaataggc tgcatgaaga cagatgaagg tgtaccgttc accggactac agttgccttc
      901 gcattttccg gcgcccgtac attcaatggc cgacaacacc gttacaaagg aaggattcga
      961 acttggacga aagttattct atgatccgat tttgtcgcgt gacaatacca tatcttgtgg
     1021 cagttgtcat gtacagggtt cggccttcac gcatcatggc catgatgtaa gccacggaat
     1081 agatgatcag gtgggcaaga gaaatgcttt accggtacaa aaccttttat ggcaaaacac
     1141 tttcttttgg gacggaggtg tacacaatat agaactcata tcactcaacc cgataaagaa
     1201 tccggtagag atggatgaaa attttccgag agcacttgaa aaattgcgta cacaccccga
     1261 gtatccttcg ttgttcaaac aagctttcgg cagtgccgaa atcaattctg aacggacact
     1321 gaaggcaatg acacagttta tggcaatgct tatatcagca gacagcaggt acgatcgcta
     1381 caaaaaaggc acagtacagt ttacagaatc agaaaaacaa ggttatgaac tgttcgtaca
     1441 aaagtgtgca ggatgccaca agggtgagtt gttcagtgat tttacttatc gcaataatgg
     1501 gcttacctct gatttttcat cagacaaggg cagatacgag atttcgttac ttgacgatga
     1561 tattggaaaa tttaaagtgc caagtcttcg aaacatcgcc aagaccaagc cctatatgca
     1621 taccggttcg ttctatacac tgcaggatgt attggatcat tacagtgatg gtgtgaaaat
     1681 gtcacctaca cttgacccac tgttgcagac tggcactaaa ccaggcatag aaatgacaaa
     1741 ggaagaaaag agcaagatta tcgattttct aaatatgctt acggacgaag gttttttaaa
     1801 gaacccaatg tttgccgaac aataatgcag aaatattgat aaatacaata agttgcaaaa
     1861 tgtgtgagat tgatatttaa ttagttatga aatttgtggc cattcagtac tgagtggcca
     1921 ttttttttgc aggattattc cgtcttgcga ataagtattt tatcaatgcg ggtaccatcc
     1981 atgtcaataa tttcaaattc aaatttatcc cagactattt tatcaccctg cgaaggaatc
     2041 ttacccatct cgttcaatat caatccgcca aatgtatcaa aattcacctc aggatctatc
     2101 tgctccaagt caaaatagtg aaggaagtcg tgaaaagggt agtggccgtc aatgagccag
     2161 gtgccgtctt cgcgttcagc aatggtataa ctgtcattgt attggtcgga agtatagcct
     2221 accatagatt cgagcaggtc attcagggtg ataattcctt gcatttgacc atattcatct
     2281 atgatgatgc catagtgtac gtgagtttct ttaaattttt caagagcctg aaatgcgctt
     2341 gtgttttcga ggatgaaatt agcttcggag gcaagtgatt ttatcagtaa cttgtcattt
     2401 ttgatgttcg aaaaaatgtc ctttaacctg gctacaccga tgacattgtc tttatctttt
     2461 tcatagacag gataaatgga gtggatttct tcggacataa taggaagtgc ctctttcata
     2521 gtgtggttgc cttgtagcat cactacctgg ctccgaggag tcatcagtgt acttacccgt
     2581 ctgtcaccta gtaaaaaaac acgttctaca atctcttgtt cgatttcctg tacttcaccg
     2641 tcttcacgtc cttcttgtat gatggctttt atttcttctt ccgtaacctt tgattcatct
     2701 gattttcgga ggccggtcag gaaaataacc agatcgagtg atttgacaag taaccaactg
     2761 aaaggataag taattcgtga gatgaaattc ataggtatgg caatgctctt ggcaataccg
     2821 tcggagtaca tgagagcaat atgcttaggc aataattcgc cgaaaataat agaaaaataa
     2881 gtaataataa ataccacgat gactacactt agtgtttcag caaatggagc taacaattcg
     2941 actttcaaca accaggtatg aatagcttca gtaatgcttt tgcctgaaaa tacaccagtg
     3001 agaatgccaa tcagtgtaat gccaatctgt accgtaggta agaaccgttt tggattttca
     3061 tgaagttcca gagctgtctg agcttttcgg ccaccttttt tggcagcagc ctcgaggcgg
     3121 gctttccttg atgacactaa tgacatttcg gccagtacta gccagccgtt tatcagaata
     3181 agtattccga ttattacaat ttccatatca aacaatctcc tgtaggatga ttctctcttt
     3241 tataatttgt aatacaaatg taatacactg aacgggactt tggcaaaatg tagtgtgaaa
     3301 aataattgta agcccaatat attctacgat tcaagcaatg atgtaaaata aaaaaaaggc
     3361 tctttttaca aagaaccttt ttgtaccgaa ggtgggaatc gaacccacac tcccttgcga
     3421 gaactggatt ttgaatccag cgcgtctacc aattccgcca cttcggccta aggctgcgca
     3481 aagttaagta aattttgttt taaacgcaaa agatacttct ttttcaaata aaaatctgta
     3541 atttttttta aagctgaatc gttttgtatt tgaaaatcaa atgattgcat caccggataa
     3601 atttcatcaa aaagctcaga ttccagattt ttaatttttg tgttaatgct gtacaatttt
     3661 tcctcatccg ggtcaagttg caagtccata acttcttcat tgatatccat catatccatc
     3721 agaaaatcct gtggcacggt agctttccct tcttcgggca gtgcatcgca cattttcagg
     3781 atatacgcca gacatttatc ctcgtctgta agtgactgat aagcctgatt aatctcagcc
     3841 gatttttcaa gtatctccga ttcgtcgtat tctgagtcga ttgtgtgaaa atcggggtgg
     3901 tacttaaaac tcagctggtg gtaccgcctt tttatatctt ccctgtcggg aaaaaagctc
     3961 accggtatct caaacaattc gaagtaattc atgacagttt agaaaaattt tctgactata
     4021 tcgagaccat tggcataaac cataagagca aggagaatga agaatcctac agtcactgct
     4081 ttttctacga atttatcact tggttttctg ccggatacta tttcataaat caaaaacacg
     4141 acatagccac cgtcgagtgc cggtataggt agtaagttca tgactgcaag tatcaaagac
     4201 agaatggctg tcatggtcca aaatcgttcc catatccaga cgtcaccaaa cattgacccg
     4261 atagaaccaa aacctccgag gctttcgctt gctttaattt tacctttaaa catctggccg
     4321 aatgccttga actggtcgcc aaggaaggag aggcctttgt tgatacctgc cggaatggca
     4381 gaagcaaagg aatatttctg agtttcgaac ttgaaatagt gatcaggcgg gtaaggcgct
     4441 acaccaattg tttttttgtc gcttgtagta agcttgacat taagtgtatc attgccacga
     4501 agcacattca gcgatattgc ctgattaggt aatttgttga ttactcgctt aaattcgtga
     4561 taatactcta ccggagtacc attgataccg attattttat cttctttttt gatgccggca
     4621 ccgtctgcgg gtccagcttt aaccacatca gccactacga aaggcatcct tgcaccgaaa
     4681 agttctgctc tgctgttctt ggcagtggcg agcattgccg ctttgtccgg gctgatatca
     4741 attaccattt cgtggccatt gcgttcgatg gtcattttgt ttatgttgtt gataatgatt
     4801 tcctttacaa gaattctgtc attaaatttg tcaaagggta tgtcgcctac tttgagcact
     4861 ttgtcgccat ccatcaggcc aagttgggtg ccgagcgaat caacatatat gccgtttttt
     4921 acttctgtgg caggaaggta tttttcaccc catacaaaaa gtaccattgc atataggaaa
     4981 aacccaagga tgaagttgac tgtaacacca cccaacatga tgatgagacg ttgccaggcc
     5041 ggttttgatc tgaactccca aggctgaggt ggttgtttga gctgttcggt gtccatgctt
     5101 tcgtccacca taccggatat ctttacataa ccacccaaag gcagccatcc gataccatat
     5161 tcagtatcac cgattttttt cttgacaagt gaaaaccacg gatcgaagaa aagataaaat
     5221 ttttcaacac gtgttttaaa ccattttgct ggcagaaagt ggcccatttc atgcagtaca
     5281 accaatatca tcaggctgag cataagttgg ccaatcgtta tcattattcc cattaactaa
     5341 ttttattgta gttttttaaa gttcaggtga ctatttttgt tgtttcaatt tttttaaagg
     5401 tgcaaaagta ctaatacaag ttcaataacc gatgcaatta ttttacacaa ctcatcagga
     5461 aggcgatttt cttatacttg aaggcgaaga agctcaccat tgtgccaatg tattgcggca
     5521 tcaggccggt catcagataa acgtaacaaa tggtaagggt agtttgttcc attgtacgat
     5581 aatagacaaa accaagacaa ctgttcgact aatgccggat tctgaacaaa aattcgagac
     5641 tccggctcat ctgaacctta gtattgccat agcacctacc aaaaacattg accgcttcga
     5701 gtggtttgta gaaaaggcta ccgaaatagg tattggtaga atcataccat tgcacacgaa
     5761 aagatctgaa cggacgatta tcaagcacga acgcctattc aagctcatga tttcagccat
     5821 gaagcagtcc aatcattttt atttacctga attacatcct ctgacaacat ttaaggagtt
     5881 gatcacagga gtaaagactg atattgcgct tattgcacat tgtatggcgg acgaattgcc
     5941 gcatgtgtca aaatttatta aacccgaccg ggatgtcctt atttgcattg gaccggaagg
     6001 tgattttaca gctgaagaag tacatacatc tgaaaatgct ggttttaccg gtgtgtcgct
     6061 tggaaaaagc cgattgcgca cagaaactgc cgggatatat gcctgcatta gctttcatca
     6121 ggctttgata atgtaaaatg tttgtatcaa ttatacgaat ttgttcgtta atactacaaa
     6181 tcgtgttttt tatgaagaag ctgttcataa gcatattctt tttattttcg tttttggcca
     6241 tttcatcagc tcaaaagagt tcgctcaagt tggcattgtt gaaatatagt ggtggtggtg
     6301 actggtattc tgttgtggat gcacttcaga atttggtgac ctttaccaac cagaatctga
     6361 ataccagttt tcaggtagat tatgctactg tggatgtagg cagtgctgaa atatttaatt
     6421 atccattgct attccttacg gggcatggca atattatact gagtgaccaa gaggcagaaa
     6481 atctcagaaa ctatgcattg gcgggaggct ttattctcat agatgacgac tatggtattg
     6541 acccatacat aaggccggtg attaagaaga tttttccgca ggcggagttg acagaaattc
     6601 ctttcaatca tccgatatac catcagaaat acgatttccc gaagggattg ccaaaaatac
     6661 acaagcacaa cgataagcct acgcaagggt ttggtatttt tatcgaaggc aggcttgtgt
     6721 gtttttattc attcgagagc aatatatcag atgggtggga gtcattcgaa gtgcacaaag
     6781 acgatcaggc catacgcctt gcagccttga agatgggtgc caatattatc caatatgcat
     6841 ttatgcagta aactaaccga cgacaaaagg gtttacaatg ttgcgattac tttattagtt
     6901 tttgttaatt atacttttgg caaaaataat tagtgcggga attcattcaa acggtgtttt
     6961 aattcttgat ttccataata atcgtgaaga aaaaccggaa aagaacttaa aatagctatt
     7021 gtcttattta aggttttgtg attttctagt tgtttattaa atatggtcaa ttgattgatt
     7081 atggtagtag gaaatgtata agtattattt acaatctgat tcattttgta taaatgtgta
     7141 gaaattactt tctcccaaat attttcgttg tagagattaa aatgttttaa ttctttcaga
     7201 cactttttat ttacagtatt cttgtaaaca ctgaagttct tacaggctac agccagaatg
     7261 tggaaagtat taaaatcttc aacatgtttc aaagcttcta ccattgggta actgatgtat
     7321 agtttgccct tttcagtttc ctcttcaaaa aaatttaaca agtttatcag cttatggtcc
     7381 cctgcgttgc ttgcatgccc atcataatca aaaaacataa agatctgtgc gaaatcggtt
     7441 cttttgaaat cttttagaat ctctttattg gcatctaaat ctttaactaa gttaaatgta
     7501 tcaagatatt cgtcttctaa aaattcattg tgaattttgt atatagtagt acaatatgca
     7561 caggtgacaa cagtattttc atttaaaaaa tatctgaaaa ggctatctga aattaatttt
     7621 tcagtttttt ggccctcaaa aataaaaagg atattattcg acattaaaag taccggcttt
     7681 atacattttt tctatgttgt gtgcttcccg taattctttt tctgtgctgt ttgaaaaaga
     7741 acgaatctgc cgtttagtca tagtgaaata acaatcaggt cttaacaagt catttgtcaa
     7801 aatagaggtg ttgtgcgttg tgagtatgaa ttgtacaccc gtttccttta atttttctac
     7861 agccattgca gaaagagaat ggtgataaaa cgcatcaaat tcgtcaacaa aaagaaaaga
     7921 aacacttgac gattctttta tggtttgaaa ccaataatag aacaaagcaa gtgaattagt
     7981 accggtagaa gcgatttcat gaaatgcaat ctttttacca ttatggaagg agaaagcaat
     8041 tgtatctctg ttcatttctt ttacaactga aagcttacac tctatacctg ctgcatttaa
     8101 aaatttttca aagtcaacca gattttttct ttttacaatg ttttcaagaa tgttttcact
     8161 tccgatatgt aacccttgat atacattatc ctgtaaagaa cggaaataca gcattttttc
     8221 tatgaatgaa aaaaaatcca gaaaagcaga attggtatcg tttttctcca gaatggtatt
     8281 gttctttaca tattttaaga ctgataattc aggattatca atcttagttt tcaaggattc
     8341 tgcgccctca aaataaattc tagcttgggt atcctttaat ctgtcaaata tggcaagttc
     8401 ctgaccatca atcaaaaaac gctcataaat aatggttttg tagtcagact tcttatactc
     8461 atagcttaca atcttcgaat taatagaaaa ttcgtaataa aacgtggcaa agccgttagt
     8521 attgaaagca tttaaataat tttaataaag gtgctcattc ctttctttgt ccgtcagatg
     8581 ttcgatgata tcgaagattg caaaagctag atttgattta ccaacaccgt tacgaccgta
     8641 aattattgcg ttattaatca agcctttttt aatggaactt ttattgaatt catatccact
     8701 tgctgctgtg agatcaagta agatgtcgtt ttcaaaactt ttaaagttag aaactttgaa
     8761 ttttcttagc atttgattta attgttaatt gcaaagaaaa ttctaattta gtttccgtaa
     8821 aaaaattacg gaaactttta attgtaattt ttataaaaat atcttatcat tacacacgtc
     8881 ctgggtatca tagttttatg ttgtacgctt tgactaagtg tagtaataaa tagattgatt
     8941 atcaggcatt tttactattc taatcaggat tccttaaatt atgcctttat tgtttattgg
     9001 cctaactcca aatggtactg cttttacgca gatagttgcg gatgttcacc cagaatgcca
     9061 ggaataggta tatgactacc ggcgatccga cagtgaggaa cgaaatgtag caaaaataca
     9121 tcctcacgcg agtgtggctg atgcctattt tgtccccgag ataactacag acgccaaatg
     9181 cagaacgttc gaccaaatct ttgagtggat tttccatgga atgatataaa ttggtgaatg
     9241 gtgtttgtta ctgcaaattt acatcatata aaagaataaa aaaagcccga ttgtacccgt
     9301 gatcagaaaa aaaatcaaag ctactcggta tttgtacgat tccacggcat ttctcttgag
     9361 ccaatgcctt agtgtagaat ctgtttcggg cacaaagtgc aattgcgtgt catgggattg
     9421 taaacgcatg ataaaatgtt gttgtctcag atcgttcata atgataccaa gttatgctat
     9481 aattattaat atgaataatc gatatcagaa tttttagtga cagaattatt ttgatgtata
     9541 tgaaatatat atcaagtata gctttctgat tttatgcata aattatttta taatgccgtt
     9601 caaccagtct gtttatgtga catagcgcta gatgattagg tggattggcg cttgaagagc
     9661 ttaagattgt agcatttttt tacattacgc attttaataa tatttaatag tctatgtatg
     9721 ttagaagttt tttataagaa atatttacat atattaattt ttggcatata atttgtactg
     9781 ttaatgttgt aggctcagcg tagttattgt ataacgaaac tgggtttgcc aactaaagcc
     9841 gctatccctc ggttttattt gtttttacat tttcccaaaa aaccgaaaat cccttacctt
     9901 aattgggtaa atttggctat gtccgaggac atagcttttt ttttacagat attttgcata
     9961 taccatgtca caataaatgc aatagaaaga ataaatatag ataaaacaaa taaaaagcta
    10021 cataaatata gataaataat aatttataaa taaataaata tttcaaatct aaatttacct
    10081 ttgtattata aatatgaaaa aataaaatag ataaaaaatc aaattataaa atacaagaat
    10141 tcaatgcata aaactgtaat aaacaaaatg gccatgaaga tcaaatttta attaatattt
    10201 gacatttaga tctcggtaca tgtatattcg agtataaatc agttaaaaat agccatgaaa
    10261 aacaactcac ttaattcgaa attgaatctc acaaaacttg tcaatgagtt aacgattatg
    10321 tcacaaaatg taaaggtaga ttctgttgag gaaacggcat tcactcaaat catcagccgc
    10381 tttcaccaag atgacaacat cgaacaggca attgcaggtt ttgaggaagc aatatataag
    10441 aacaggctta ccattgcctt tatgaagaaa aaaatatcac agttaatcga aaataaaatt
    10501 gcagacttgg ctatcgaaac aatcagtctt gctacacagc ttgccccaca cgatcgcgag
    10561 ttacattttt tgatgattca ggcatgcaga ctgaatgaag attactcacg agcgcttgat
    10621 atacttgata cgctaaaggc cgatgctaca cctcgagaag taatccttat actgaatgaa
    10681 gaggctgcta tatataagct gatgcaaaac gcagaaagta cttttgtctc actcagaaga
    10741 ctactcacca tcgatccgca taacgaagat gccctgcgag aaatctggtg gagtatcgaa
    10801 atgtcaaaaa actataaaga aagccttaag ctgcataaaa tcgttattga gaaaaatcct
    10861 tacagtgcta gagcatggta taacctggca catgccgaat actacctaca ccaatatgaa
    10921 aatgccatta ggtcgtttga gtatgcatat atcatcgacc caaagttcga actcgcctat
    10981 cgttactgtg ctgaagtgtg tacacttacc ggacgattcg aacaggctct taaatgttac
    11041 tacgatctta tggagcatgc cgttgccgat gccgaagcgc tcaaaagcat cgggtattgt
    11101 tacgaaaaaa cttctaacct gatcaaggct agaaaatact atctactggc acgaaacctc
    11161 gatccggtgg atgacgaaat atactataac ctgggtaata catacaaggc agatggtcaa
    11221 tggcagcttg cagccaatta ttacgaacgc gctgtttctc ttcaagaccg caacgaagac
    11281 tatttcgtcg ctctggctga agcatatata cactgtggcg aaaaccacaa agccgacctg
    11341 ttcttccgta gagccacaga ggtttgtcct gaaatgccag ccccttgggt acgttatgcc
    11401 gaattttact tcaagggcgg aaaatctgaa aaagcactcg agattcttct cgaagcggag
    11461 gaaacaacgg ccggtgccga aatttactac tgtcgggcta tcattttatt ctcattaaat
    11521 gaaaaattcg atgccatgga aaatctgggt gaagcactcc agatcgactt tgacatgcac
    11581 aagatattat ttgactacct tcctgcatta aaggaagatc gcgaaatcaa ggcgattatc
    11641 aaatactatg agtttgggta aaacttactt ataatcctat gacacaaacg gttcggacgc
    11701 catcgatgtc cggtaatgag gggaagaaat tcccctttat tattttatgc caaaaccaaa
    11761 tatgctcagc ctgtgttaaa ctgcctggct ttggcttatc tttgtactga aaagcagtat
    11821 ctatgaaggt cgccggattt acttttatta aaaatgcagt caaatttgat ttcccgataa
    11881 aggaagcgat tttgtccatt ctgcctattt gcgatgaggt agtggtggca gtgggagatt
    11941 gcgaggatgg tacgctcggg cttattcaat ccataggttc tgataagatc cggattatcc
    12001 gtacggtttg gaatcctgaa cttactaccg gaggcaaagt actggctgac gagacgaaca
    12061 aagcactagc agccgtaagc ccggatgccg actggtgttt ctacatacaa ggtgatgagg
    12121 tatttcatga agatggtata gaaaaagtac agcaggcaat gctgcaatat aaagacgaca
    12181 gcacggtcga tggcttgttg gttaaatatc ttcattttta tggctcctac gattacattg
    12241 caacctcaag gcattggtat cgaaatgaaa tacgaatcat taaaaacagt cggagtattt
    12301 tcagctataa ggatgcccag ggttttagaa aaaacaatga cgaaaaactg tacgtagtac
    12361 cggttgacgc atttatgtac cattacggat gggtcaagga accgaagata atgcacaaaa
    12421 agatgcagaa tgccgacacg atatggcatt cggcaaagga taatgaagct cggcagatac
    12481 ccgtagttgc ggaattcgat tattcgggaa tcgatatgct ggctacgttc gtgggcaagc
    12541 acccgatagt aatgaaagac cgtatcagca gcaaaaactg gcattttgag tatgacctgt
    12601 cctacaattc gatgagtcta aaggagaaaa tcaaggatct ggtttatcgg cttaccggtc
    12661 gcgagttatt ttcttataag aattacaggt taaagtgatg agctaaaaaa gaaagaatat
    12721 tcggaataaa actttgaaat aactttattt ttggtttcaa tactgagtaa atattttaaa
    12781 ttatttactt gttcaatgtt tagaattggt tatatcgtga atctcatttc atttattaat
    12841 acgtaataat ggaattatat ttcaatgcaa ggaggatgag tagtagtatt gtcagttcaa
    12901 tgtaaaaatt gtaaaaaata tttaagacaa atttgtcgga aataaaagat tggcttacat
    12961 ttgcaccgca aaacggcaac agcaaatgtt ttcgaagtca tgtgaatacg gtattaaagc
    13021 catcatatat attgccaccc aatcgcttga agacgtgcgg gtaaagattg gcgacgttgc
    13081 tgagaatacc ggtacacccg aagcctttac tgccaaaatc ctcggttctc ttaccaaaca
    13141 caacatagta tcgtccatca aaggcccgcg tggtggcttc gaaatcgata aatcgagaat
    13201 gaaaaatata atggtgagcg agatagtagc tgccctcgac ggcgatgcca tctataacgg
    13261 ttgtgccctt ggcctgagca attgcagcga tgttgacccc tgcccgctgc acgacagcat
    13321 cgttgaggtg agaaacaaac ttcgtaaaaa tctagaatca accacggtgt atgacctggc
    13381 aaccaagcta aaatccggaa aatcgattct gataaggtaa aaaatctgta acatttatga
    13441 acaaatgtga atcaaaataa ttatacgtat aatattgaaa aataatattt taaccgaaaa
    13501 acacattgat aaacacctaa aatacaaaat taattgatta tggaaaagaa agaaatctgg
    13561 ttggagtcgc tcataaccga aacaccgcag gaaggtcgcg aactggctat taaaatgtca
    13621 aggaaaacta ttgcagccat tcagacagac cctgaagtaa gaaagaaact aagacccgac
    13681 tatgccaatg atactgccca gcttatagct tctgccaatg tagtagccat tgagtttcag
    13741 accattgcgc aagctaataa ttactggaga aaataatgct gatacagaaa cctggtaata
    13801 gccttacgtg aatgaaatga taccggagga tgttttccgg cttaaattgt tagaaatatt
    13861 aaaacaatag attaagattg accgaatgac tgaaacaaaa gaaatagaaa acctggaaga
    13921 tatacagctg atggttgaca gtttttatgg aagaataagg caagacgaaa tgctcggtcc
    13981 tgtattcaat aatgtgatac aagacaaatg gccggcacac cttgagaaaa tgtaccgttt
    14041 ctgggaaaca gtattgctcg acaagcattc gtactacggt gcaccttttg taccacatgc
    14101 caagctaccg gtacaaaaag aacattttga ccggtggata gatttgttct cagccacagt
    14161 tgacactttt tttcacggtg aaaaagcaga gcgagctaaa tggcagggca acagaatggc
    14221 agaaatgttt ctgatgaaga tacagtacca taggaacagt tcatccatcc cactcatcta
    14281 ataattgaga aattaaaacc ggaggaatca ttttcaaatt gatcaaaatt taaaattaat
    14341 atcatggata tcacaaaaga aagcatcatc ggcgaactcg tagcaaacga ttaccggact
    14401 gcttctgtat ttaagaagaa taacatcgac ttctgctgtc aaggcaaccg caccattgag
    14461 gtagcttgca aggaaaagca acttgatacc gacaagctcg taaaagagct ggccaacgct
    14521 gccacgcaga tacaatcagg ctctgttgac tacaaatctt ggccactcga cctgcttgcc
    14581 gattacatcg aaaaaacgca tcaccgctat gtaactcaaa agtcggaaga gattatgcct
    14641 ttcttggata aaataatccg tgta
//
LOCUS       DAOQQY010000036        14236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1393_length_14236_cov_7.036598, whole genome shotgun sequence.
ACCESSION   DAOQQY010000036 DAOQQY010000000
VERSION     DAOQQY010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14236
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1393_length_14236_cov_7.036598"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLE23_03475"
     CDS             <1..493
                     /locus_tag="PLE23_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HMX82357.1"
                     /translation="LTHDKPQNPKDSRLYKWIKKIYPVSRSVYGGAFFMKRGKIRFAT
                     PLFVVVILIELTDLFFALDSIPAILAITADPFIVFSSNILAVMGLRSMYFLIVGMLDK
                     FKYISYSLIVILAFVGIKMILSHHVDIPDWLSLSVIAFSLGAGMVASTISEKKAAKSL
                     TEG"
     gene            885..2711
                     /locus_tag="PLE23_03480"
     CDS             885..2711
                     /locus_tag="PLE23_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82358.1"
                     /translation="MTQQDLKKKQILQSVLLICLCTTINISLKADGAIPRNGILSTSS
                     IHFFYPVDDTLKVPTVGKKDFGFSGGASVFASTYNSNIQNISSSPFNYGISLATSLKF
                     KKNSFPFSLSYSNNKLNVSYPYFRIGCAPSYKWMKLYLGNQTLSYNRYVFSGINVFGV
                     GFEIHPGWFYLSAISGNLAKKIFVDSLSTSYNNVRPRFSANGFAIKSGVKTRSFQLLF
                     TYFSGNEDKTSIRYSNPKYFLTPRMGKALGAEMGIRFSKYVNFSSIGGLSIFTRNKDA
                     GNLDTLLKASQAQPLPAWAKIIEGKPNTTTQIFYAFENSLRYENKIFSLGIKQKRIMP
                     EFKNLGLRIEGNDIDQYTIEPGFTFWDGKLTSNITVGIQNNNLLSKSALSTNSKIYEV
                     SVNYAANENLFLNASFSNFGIQTSSTSIASEDSISIRNVSSNIGLSSNYVLEQNADIN
                     RSLSFNLNNQRTTEIYENSKFNNSDFNSFFANLAYSVSKPHGLNYNVGLNYSKSGTLL
                     AEFNNKILDVQMYGMFGGVSMNIFQNKTKDRSLTIGAGTNLNYSGLAGGKKSLATGLG
                     LNLIFAFTKNMTFTAGYTHSSNTIEHVRLLQNSFNTNIQTNF"
     gene            2713..9060
                     /locus_tag="PLE23_03485"
     CDS             2713..9060
                     /locus_tag="PLE23_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82359.1"
                     /translation="MKTSNNQTVGIGSSNCKEGGFVSAKITKNIFLVARFFLSALFLI
                     CIQTAVYSQNQLKINVQVQPPYSPFLNDYLGLKDKTVLTVTNTTSQTIQYYLKVSLTN
                     NDNFSAKTKDDYKPATPMTIQGGQTVMIQASSSAGSSFSEENIEVDYGDYTLSDVLRD
                     GVLPDGKYNLCVTAFDYTNDQQISSSATGCKLINISYLNAPRILACDNKSFTQTMPQN
                     INLSWSPVTGNNQNKQILYDLYIAPITEADNPQDIIETGIATGSPNLIKVSGIVVNAY
                     TYSVNKPALTIGKYAWAVKAAVADKSLPIENDGLSNVCTFDYKPMEMKFNNVQMNVGP
                     ECKCKSQLPNGLDPIQGSPAVGQIVNSGFLKVTLTAVSKSDNGYTGTGTVPLPILNNG
                     IVKVNVSFTNIDIKKSGNEYYQTSGIIEAVMDEKASFLPKFNPIDPGSLNLDPQQMQS
                     LSSFFENYSNQLISKIKNYPNTIAYNLPIGLDEKAMNIAITGLTLAPEQSYFDAVSVL
                     DIIDGNTKVALIGQGICIDAGSFCGNATLSLMEDFNVPSIGISLKGNGKNDGTSISFD
                     KDGFKNLHIAATYTFPDNSLVEVASQKPAAVTLTSDTDSGWSDWLAEVKFSEFYMAGF
                     PDVTFGPDANGTKIMYDHSDKRNPADIPSPYTSEDNADAPINTNQPTWRGFYIPSVAI
                     KLPPSLTNVNGNPLVVKAEKLIFDGGLSGNVSVNNIMSINDGSLDGWYYSIDQFQINF
                     WKNTFKSSSLSGKIVLPISRKYDDTSNQLDYTCTLSKPANSSLNYNFVISPKDNIAFN
                     VLWANCKFKPGTNIQITKTNDGKFVAMATLNGQMTITSKIDNLPDVNIADVKFENLRF
                     QSQKPYFTPGQIDACLFSTASPQHSIAGFSINFDPTPGKGISLYSNANNGVQLGLKFN
                     AQLKLVKDVDFVPKADVSFNIFGNLKMNGTRPEWDGVGANISKIKFEAGAKIGPLGII
                     GEIAYFNDNNGSYGFMGGLTMDAAGVVTIGAKAQFGYSANEGGFNYFFIDGMVDFKSN
                     GIPMGPALAIYGFGGGAFYNMTIPQVNMNGANITGSPAYVFDPNDPQAGVSMSGIVYT
                     PKKGLFSVKAAVLFGLTARNVLDADGSVTMNFNTNTGSVLSIDFQATGRFITKADDPI
                     PIKNTECMGKLTIYMQLNFDEASILFHADIKAGVPTHTDINLFYIDAGIDFYAGRSGW
                     FVHVGTPWKDGNLKGGTPITLTVFNKLKFLSYFECGTGSGKIFYKNTIVSGVNAVDPM
                     PPIPNYIYDIIYNNNRSGENGNIDNSVNSFSFSKNDLKGGLAFGANFSTEINVNFLLF
                     YLEAKFMVGFDLAFYELQKGAMCVNEKNEKMFKGVNGFYAMGQAYFGASIDVGIDIDL
                     FFFSGKISIISAGVAAYVQFGAPSPSFATGAIGGRYSLLGGLIEGHFGVKFFWGEKCY
                     EIEDESIDLIAEVNPTADFDKEHYQTPVKASQLQPVNLDPYVVFNFEIDRTFLIIANV
                     DEKDPDNPGATYRQYRYYHILPEDISINVSGGLIEQNGVPLPKPLSINDFKVSNDHFV
                     LKLKEPVMFRKETDFVFDIKLPVRIFNLRERSKQQAATSQGYKTDAELAGVNNFVRNQ
                     WDLATDANLQKYYDQRHVQFTTDCGIKYLEQSWITDIDPLHRSQNNPAGYSAAFNEKN
                     LRKGTYFTPTYTYSINKPYAQQLISNTSKNNVKLSLEHPEINLSLDNGYLSNDFLCIP
                     PDFVNNSDITLKISTFEKRNSGNTFKSYLVDAVVSADKRSITANLDKDFPKNSFVIVQ
                     MVLKKRTNPNSNNRPESSLVTKKLITNNEANVCTTIMTRTANLDAKLKSASELELFKW
                     YFTTGSYTSYPDKMADMDIKVDTVTTRYNLIGGNKGMVASASFEAVVSTYQFKGNEKF
                     DFHDVYDYNLNQVLTDGISKPFPKKLADGYLGFAFDNASQTLVDDFLRTYFDNEMVMK
                     QFLKLYPDDYLHDTPTGQGGHVKLMNLFITQRMKDLTRWENVTKVGAVLPPLDYLPSE
                     LLYKGADFTGMDMGQLNIKKQILFSVSKNSRIKSYLGGHTKIIPAHIIGLNDIPNFKP
                     GNGPVIFQAFMADMMQDFNNNILSKVAGAFNPMILNNKQSR"
     gene            9062..11131
                     /locus_tag="PLE23_03490"
     CDS             9062..11131
                     /locus_tag="PLE23_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMX82360.1"
                     /translation="MKNLKIVFCTMLFFIATLSFGQQAEVPKISLKYAIMAKTFKDSI
                     VLRWAPESPNALPAHIDAGVWVEKLVVSGKFPYKLGKWQRITPTPILPQPLEAFNTEA
                     CKKDEYTMLGAQLLYGKLPSIPTHTGFKGVKDQTEMLTNLFSLAMLGCDYSPKAAQMM
                     GMRQVVKVSIAENEKIFFRIYSAFDNPMFQIDTGMTFTTYGEWGAQTAPKFLTAKSQE
                     KMVELVWPCNRTLNRWSGFNIERSGDGKNFTRLNKKPFIIMSPELDINMYYRDSVSNY
                     VKYYYRIQALDPFGDVTDFSESVSCYGSDKTPPGRLVLEENHGDGSGITLKWRFENNQ
                     PAVDLDHFVVKTGPGVDYIHDTLKILPNSAASFEYPVRAKVKSTYFEVAAVDTAGNYS
                     SSNPVRYFIPDTEPPKAPVGFKATIDTAGIVRLSWQLDTTDEIQGYRVFRKNDPKHEF
                     TCMQQGFLKETVFTDTLALNTLTKDIYYAVATIDMSYNMSKMTETIKLIKPDKAKPVP
                     PQITHYKLRDRSVTLQWTQSPSDDVDYYQLYRTDEKGSTVLVAKNLRADQLEYTDSAL
                     TAKARYTYYLNSTDKSGLTSENSFPLDILAYTNNPGKIIALTWLSEKEKFGFKWTDPN
                     PKAAFYIIYKDTGSGLQQYKSIDSDQSKFTEETNKQTKVRFGLQAVYQNQQKSGIYSL
                     EWYKPEH"
     gene            11164..>11968
                     /locus_tag="PLE23_03495"
     CDS             11164..>11968
                     /locus_tag="PLE23_03495"
                     /inference="COORDINATES: protein motif:HMM:NF023048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-binding motif-containing protein"
                     /protein_id="HMX82361.1"
                     /translation="MKNVLLFCALCLLHPSIFSQQIFFEDFDTYTLGPIGDQNPEWVN
                     LSNAQVNVKIDGGNFVNCSGSDNQNAYLSFESADFEECKRIVSTSYSNFVLDMKATLN
                     SGISVKIVDTDDFAFEYLYEFDGSGNFEFAFILDFVYHQIQVIRNGVTVKTDNLPAGF
                     NSIDEIHFNNSTGKFKLGCVELSDVTDVDGDGYLASNDCDDNDANINPGKAEIAYNGK
                     DDDCNPATLDDDLDQDGYPKATDCNDDDADINPGKAEIAYNGKDDDCNPA"
     assembly_gap    11969..12068
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12069..12451
                     /locus_tag="PLE23_03500"
     CDS             <12069..12451
                     /locus_tag="PLE23_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MopE-related protein"
                     /protein_id="HMX82362.1"
                     /translation="DLDQDGYPKATDCNDDDANINPGAAEIPGNSIDENCDGILLAEF
                     HQSTKLFVELIPNPTRDYLEIKSSSKIDYVKIYNIAGSEVSSYKINESNIIYLKDIRE
                     GIYFIHAFSSESSGSYIGKFVVNY"
     gene            12792..>14236
                     /locus_tag="PLE23_03505"
     CDS             12792..>14236
                     /locus_tag="PLE23_03505"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMX82363.1"
                     /translation="MKKLTILIYFFSFVVWNLKGQTLSFKTYNSNSGLSQNSIYSITQ
                     DKEGFLWFGSQAGLNRFDGNEFKLFVPRIVTNHKVMTDFSKMITALHRDATNFLWVGT
                     TNELLIYDAVKNKWYTPEEKYPGFYIAPLAWVVAVTEDAGGKLWVSTRKAGLYCYDKI
                     KKKMIVLPKQIVQAKPEIKHCRFDNDHICTARDNVIYLLNDTSVQLINTDKLLQSNGN
                     IVTAVESVNGDVWFIANGKSIYSYNLGSSKLTRHTLNFDNKQYLKDLSSLYFSDDNTL
                     WISSRTSGLVRYNIITSEADYAKAGNEENALKKNFILSIFQSEDGLIWVGTSGGGVSK
                     CEVNKAGFQLYHVENKGSPEESYDNMILSAYTDDETEFYMGTSYAGLLKYNIRTKQFH
                     YFAPFTSSNNVPQSNNIYSVIKGDKTLLWLATWGGLYSFDKLTHRFKGYFDKKNEFTR
                     ELNCAIKLKLSNKILICSSKGIPLLFDTDKK"
BASE COUNT         4568 a         2658 c         2803 g         4107 t
ORIGIN      
        1 acttacacat gacaaaccac agaatccgaa ggactctcgg ctgtacaagt ggataaagaa
       61 gatttatccg gtgtcaaggt ctgtgtatgg tggagctttt tttatgaaaa gaggcaaaat
      121 tcgctttgca acaccacttt ttgttgttgt tatcttaatt gaattaactg atcttttctt
      181 tgctctggac agcatcccgg ctattcttgc tattacagcc gatccgttta ttgtgttcag
      241 ctcaaatatt cttgccgtta tgggtttgcg atccatgtat ttcctaatag taggtatgct
      301 tgataagttt aaatatatca gctatagcct tatcgtcatc cttgcattcg tgggtatcaa
      361 aatgatactg tcacaccatg tcgatatacc agactggctg tcattgagtg tgatagcttt
      421 ttcacttggc gcaggaatgg ttgcttctac tatatccgag aagaaggcag caaaatcgct
      481 tacagaaggg taaatgtaat attcagttga acctaaaatt tacgattgta cgaagcttta
      541 tttaaccgtt gccgtacaac tacgttgaat ggtattgcct gcaaattatc attagtgtgc
      601 ctgttaatat aacagaatgc ctttacggaa tctaccaaaa ttcaagttag gacctaatat
      661 tcaagcaata attgaatact tactgtatga taacttattg tggaaatcat gtatcttcta
      721 atgtagttcc tgctcaatag aatagtcttc gataaggctt taccatttac ccaccatttg
      781 acgccatcca ctaactcggc ttgtactgtt actcacttgc caaactttaa ctgaataatg
      841 cgaatacatt tgtatcgtag aaaaatttca gaaattaaag tagcatgacc cagcaggacc
      901 ttaaaaagaa acaaatattg caaagtgtat tactgatatg cctttgtaca actattaata
      961 tatctttgaa ggccgatgga gcaataccta gaaacggtat tctcagtaca agctcaatac
     1021 actttttcta tccggtggat gatactctga aggtacccac ggtaggtaaa aaggacttcg
     1081 gattttcagg aggagccagt gtttttgcct ctacttacaa ctccaacatc cagaatatct
     1141 cttccagtcc attcaattat ggtatatcct tagctacttc tctgaaattt aagaaaaatt
     1201 catttccttt ctcactcagt tatagcaaca acaagctcaa tgtttcatat ccatacttca
     1261 ggatcggatg tgcaccatcc tataaatgga tgaaactgta ccttggaaat cagacgctga
     1321 gttataatcg ttacgttttt agtggtatca atgtctttgg cgtcgggttt gagatacacc
     1381 ccggttggtt ttatttgtct gccataagtg gcaatcttgc aaaaaaaatc ttcgttgata
     1441 gtctctccac atcttataat aatgtgcgcc ccaggttttc agccaatgga tttgcaataa
     1501 aatctggagt caagacccga tcgtttcaat tattattcac ctatttttca ggtaatgaag
     1561 ataaaacttc catccgctat tcgaacccaa aatatttctt gacaccacgt atggggaaag
     1621 cattgggtgc cgagatgggt atcagatttt ctaaatatgt caacttttca tccattggcg
     1681 gtttaagtat ttttacaaga aataaagatg ccggtaatct tgacactttg ttaaaagcat
     1741 cacaggcaca accattgccc gcatgggcca aaataattga aggcaaacca aataccacta
     1801 cacagatatt ttatgctttc gagaattcac ttcgatacga aaataaaata ttttcattag
     1861 gcataaagca aaaacggatc atgcctgagt ttaagaacct cggattgcgg attgagggaa
     1921 atgacattga ccaatatacc atagaacccg ggtttacatt ttgggatggt aagcttacgt
     1981 caaatattac cgtaggtatc caaaataata acctcttgtc aaaatcagca ctcagcacaa
     2041 atagtaagat ctatgaagtg tcagtcaatt atgctgctaa tgaaaatttg ttcttgaatg
     2101 catcattttc caattttggc atccagacct catctacttc catagcatcc gaagatagta
     2161 tttctatacg caatgtaagt agtaatatag gcctgagtag caattatgtg cttgagcaga
     2221 atgcagatat taatcgttca ctgagcttta atctgaataa tcagcgaacc actgaaatat
     2281 acgagaatag taagtttaac aactctgatt tcaattcttt ttttgcaaat ctggcttata
     2341 gtgttagcaa accacacggg ttaaactaca atgtaggcct taattatagc aaaagcggaa
     2401 cattgctggc agagtttaat aataagattc tggatgtaca aatgtatggc atgtttggcg
     2461 gtgtcagtat gaatattttt cagaataaga caaaagacag atctttgacc atcggtgctg
     2521 gtactaacct caattatagc ggcttggctg gcggtaaaaa gagccttgct acaggacttg
     2581 gcctaaattt aatttttgca ttcaccaaaa atatgacatt cacagcaggg tacacccatt
     2641 cctcaaatac aatagagcat gtgcgactgc ttcaaaatag ctttaatacc aacattcaaa
     2701 caaattttta atatgaaaac atcaaataat cagactgtcg gaattgggtc cagtaattgt
     2761 aaagaaggag gatttgtttc agctaaaatt accaaaaata tttttttggt tgcacgtttt
     2821 tttctgtcag cattgttcct catttgtatc cagacagctg tttatagcca gaatcaatta
     2881 aaaataaatg tacaggtaca gccaccatac agcccttttt tgaatgatta tcttgggttg
     2941 aaggacaaaa ctgtacttac tgttacgaac acaaccagtc agaccattca gtattatctc
     3001 aaagtttcgc ttaccaacaa cgataatttt tcggcaaaaa ccaaggatga ctacaaacct
     3061 gccacaccta tgaccatcca aggcggacaa acagttatga ttcaggcaag ctcatctgcc
     3121 ggtagctcat ttagtgaaga aaatattgaa gtggactatg gagattatac cctgagtgat
     3181 gtattgagag atggtgtgtt gccggatggc aaatacaatc tgtgtgttac agcttttgat
     3241 tacactaatg accaacaaat aagcagtagt gccaccggat gtaaattaat taacatctca
     3301 tacctcaatg cacctcgaat actggcatgt gacaataaat catttacaca aacaatgcct
     3361 cagaatatca atcttagttg gtcgccggtg acgggaaata atcaaaacaa acagatattg
     3421 tatgatttat acatagcacc tataaccgag gcggataatc cacaagacat catcgaaacg
     3481 ggaatagcta ccggttcacc aaatctgata aaagtgtcag gtatcgtggt taatgcttat
     3541 acttattctg tcaataagcc agcattgacc attggcaaat atgcctgggc agttaaagcc
     3601 gcagttgctg ataaatcatt gccaatagaa aacgacgggc tgagtaatgt ctgcactttt
     3661 gattacaaac caatggaaat gaagtttaat aatgtgcaga tgaatgtagg acctgaatgc
     3721 aaatgtaagt ctcaattacc taatgggctc gaccccatac aagggtcgcc ggcagtaggg
     3781 cagattgtca attccggttt tttgaaagtg actttgacag cagttagcaa gagtgacaat
     3841 ggatatacag ggacaggtac agtacctttg cctatcctca acaatggtat tgtaaaagta
     3901 aatgttagct ttacaaatat agatattaaa aaatcaggca acgaatatta ccaaacttca
     3961 gggataatag aagcagtgat ggacgagaag gcatcgtttt tgccaaaatt taatcctatt
     4021 gatccgggaa gtctaaacct tgacccacaa cagatgcagt cattgtcatc tttttttgaa
     4081 aattactcta atcaactgat ctcaaagatt aaaaactatc caaatacaat tgcttacaat
     4141 ttgccgatag gcctcgatga aaaagccatg aatattgcaa taacaggctt gacattggca
     4201 cccgagcagt cttattttga tgcggtatca gtattggata ttattgatgg taatacgaag
     4261 gtagcactta taggccaagg catctgtatt gatgccggta gtttctgcgg caatgcaaca
     4321 ctttcattga tggaagactt caatgtacct agtataggta taagcctgaa aggaaatggt
     4381 aaaaatgacg gcacatctat ctctttcgat aaagatggct tcaagaacct acatattgca
     4441 gctacctata ccttccctga caatagcctg gtagaagtag caagccaaaa accggccgca
     4501 gtgacgctta catccgatac cgattcgggt tggagcgact ggcttgccga agtaaagttt
     4561 tctgaatttt atatggctgg ttttccggat gttacatttg gcccggatgc taatggtaca
     4621 aagataatgt acgaccacag tgataagaga aatccggcgg acattccgag tccatataca
     4681 agtgaagata atgccgatgc acctattaat acaaatcagc ctacttggcg aggattctac
     4741 attccttcag ttgctataaa attgccacca tctctcacca atgtcaatgg taatcctctc
     4801 gtggtgaaag cagaaaaatt gatttttgat ggagggcttt cgggtaatgt ttcagttaac
     4861 aatattatga gtatcaatga tggctcacta gatggctggt actattccat tgatcagttt
     4921 caaattaatt tctggaaaaa cacctttaag agtagcagtc tgagtggcaa aatcgtgcta
     4981 ccgatatcaa gaaaatacga tgatacaagt aatcaacttg attatacctg tacattgtcc
     5041 aagccggcca acagttcatt aaactataat tttgtgatat cgcccaaaga taatattgcc
     5101 tttaatgtat tgtgggcaaa ttgcaaattc aagccgggca ctaatattca gataactaaa
     5161 acaaacgatg gcaaattcgt agcaatggcc actttaaatg gtcaaatgac aattacatct
     5221 aagatagata atctaccaga tgtcaacata gcggacgtta agtttgagaa tttgagattt
     5281 cagagtcaga aaccgtattt tacacctggc cagatagatg catgcctgtt ttcaacagct
     5341 tcgccgcagc attctatagc cgggttcagt attaactttg accctacacc tggcaaagga
     5401 atatcactgt attcgaatgc caataatggt gtacagcttg ggctaaaatt taacgcccag
     5461 ctcaagctag taaaagatgt tgattttgtg ccaaaggcag atgtgtcgtt taatattttc
     5521 ggtaatctta agatgaatgg caccagaccg gaatgggatg gggtaggggc taatatttct
     5581 aagattaagt ttgaagccgg tgctaaaatc ggaccccttg gaatcattgg tgagatcgca
     5641 tattttaacg acaacaacgg aagttatgga tttatgggtg gcctcacaat ggatgctgcc
     5701 ggtgtagtga ccataggcgc caaggcccaa tttggctatt ctgccaatga aggtggcttc
     5761 aattactttt ttatcgatgg catggttgac ttcaagtcaa atgggatacc catggggccg
     5821 gctttggcta tctatggttt tggaggaggc gctttttaca atatgaccat accgcaagtc
     5881 aatatgaatg gggcaaacat cacaggttct ccagcttatg tatttgatcc caacgaccca
     5941 caagcaggtg tgtctatgtc gggtattgta tatactccaa aaaagggatt gttttcagtg
     6001 aaggcagcag tcttatttgg cttgacagca cgcaatgtac tggatgctga cggaagtgtc
     6061 accatgaatt ttaacactaa caccggaagt gttctgagta ttgattttca ggccaccggc
     6121 cgatttatta caaaggccga cgacccaata ccgataaaaa atacagagtg tatgggcaaa
     6181 ttaaccatat acatgcagtt gaattttgac gaagcgtcaa ttttattcca tgccgacatt
     6241 aaggcaggtg ttcctacaca tacggatata aacttattct atattgatgc cgggatcgat
     6301 ttctatgcag gtcgttcggg gtggtttgta catgtaggca caccatggaa agacggaaac
     6361 cttaagggtg gtaccccgat tacacttact gtatttaata agctcaagtt tttatcatac
     6421 ttcgaatgtg gtactggttc aggtaaaata ttctataaga acacgatagt aagtggtgtg
     6481 aatgctgttg atccaatgcc gccgataccc aattatattt atgatataat ttataacaat
     6541 aacagatcag gtgaaaacgg aaatatagat aactctgtca atagctttag tttttcgaag
     6601 aatgatttaa aaggaggcct ggcttttggt gcaaacttta gtactgaaat aaatgtaaac
     6661 tttctgttat tctaccttga agcaaaattt atggttgggt tcgaccttgc attttatgaa
     6721 ttgcaaaaag gcgctatgtg cgtaaatgaa aagaacgaaa aaatgtttaa aggcgttaat
     6781 ggcttctatg caatggggca ggcatatttt ggtgcaagca ttgatgtagg tattgatatc
     6841 gacttattct tcttttcagg taaaataagt atcatctcgg caggagtggc tgcttatgtc
     6901 cagtttggag ctcctagccc atcttttgct acaggtgcca ttggcggacg ttatagcctg
     6961 ctcggtggtt tgattgaagg ccatttcggt gtaaaattct tctggggtga aaaatgctat
     7021 gagattgaag acgaatcgat tgacttgata gctgaggtga atcctaccgc agattttgac
     7081 aaagaacatt accaaacacc ggtaaaagcc tcgcagttgc agcctgtcaa tctagatccg
     7141 tatgtggttt ttaattttga aatagaccgt accttcctaa tcattgccaa tgttgatgaa
     7201 aaagacccag acaatccggg tgctacttat cgccaataca gatattatca tatattgccg
     7261 gaagatataa gtatcaatgt atcaggtggg ctgatagaac aaaatggtgt acctctacct
     7321 aagcctttgt caataaatga ctttaaagta agtaatgacc attttgtgct taagctaaaa
     7381 gaacccgtaa tgtttagaaa ggaaacggat tttgtttttg acataaagct gccggttaga
     7441 atttttaatc tgagagaacg ctcaaaacaa caagccgcta cttcacaagg ctacaaaacc
     7501 gatgcagaac tcgcaggagt taacaatttc gtccgtaatc aatgggacct ggcaacagat
     7561 gcaaaccttc agaaatatta cgaccaaaga cacgtgcaat ttacaacaga ttgtggcatc
     7621 aaatacctag aacaatcctg gataacggac attgatccct tgcatcgttc gcaaaataac
     7681 cctgcagggt acagtgcggc atttaatgag aaaaatttga gaaagggaac ttacttcacg
     7741 cctacatata catattctat caacaagcct tatgctcagc aattaatatc caatacgagc
     7801 aaaaataatg taaaattgtc attggagcat cctgagataa accttagcct cgacaatggt
     7861 tatttatcca atgactttct gtgtatccca cccgattttg taaataattc tgacataacg
     7921 ctcaaaatat ctacattcga aaagcggaat tccggcaaca cattcaaatc gtacttggtg
     7981 gatgcagtag tatctgccga taaacgcagt ataactgcaa atcttgataa ggattttcca
     8041 aagaatagtt ttgtcatcgt ccagatggta ctcaaaaaac gtacaaaccc gaatagtaac
     8101 aatagaccgg agtcatcact tgtgacaaaa aaacttataa ccaacaatga ggcaaatgtg
     8161 tgcacaacca tcatgaccag gactgccaac ctcgatgcga agttgaaatc cgccagcgaa
     8221 cttgagttat ttaagtggta ttttacaacg ggtagttata ccagttaccc tgataaaatg
     8281 gcggatatgg atataaaagt agatactgtc acgacaaggt ataatttgat aggcggaaac
     8341 aagggaatgg tggcctctgc ttcgtttgaa gctgtggttt caacttatca attcaaaggc
     8401 aatgaaaaat ttgattttca tgatgtgtac gattataatc tgaatcaggt actgacagat
     8461 ggaatctcga agccgttccc gaaaaaactg gccgatggat atctgggatt tgcatttgac
     8521 aatgcctcgc agactttggt agatgatttc ctaagaacct actttgataa tgaaatggtt
     8581 atgaaacaat ttttaaaatt gtaccctgat gattatttgc acgatacacc tacgggtcag
     8641 ggcggccatg tgaaattgat gaatctgttc atcacacaaa gaatgaagga tttgacgaga
     8701 tgggaaaatg taactaaggt aggtgctgta ttgcctcctc ttgattatct cccatccgaa
     8761 ttgctttata aaggtgcaga cttcacgggt atggatatgg gtcagcttaa tattaaaaaa
     8821 caaatcttat tttctgtttc aaaaaattct agaatcaaaa gttatcttgg gggacatact
     8881 aagatcattc ctgctcatat tattggatta aatgatattc cgaattttaa gcccggcaat
     8941 ggtcctgtaa tattccaggc cttcatggca gatatgatgc aagattttaa caataatatt
     9001 ctttcgaaag ttgcaggtgc ttttaacccg atgattttaa acaataaaca atccagataa
     9061 gatgaagaac cttaaaatag tattctgtac gatgttgttt tttatagcaa ctctttcgtt
     9121 cggccagcag gctgaagtgc ctaagattag cttgaaatat gctataatgg ccaaaacatt
     9181 caaagatagt atagtgctgc gctgggcacc cgaatcacca aatgccctcc ctgcccatat
     9241 tgatgcagga gtgtgggtcg aaaaactcgt ggtaagtgga aaatttccat acaaactggg
     9301 taaatggcaa agaattacgc ctacacccat tttgccccaa ccactcgaag cgtttaatac
     9361 cgaagcttgt aaaaaagacg aatataccat gctcggtgca cagctcttgt atggcaaact
     9421 tccttctatt cccacacata cagggttcaa aggggtgaaa gatcagaccg agatgctaac
     9481 caacttattc tcattggcaa tgctcgggtg tgattattcg ccgaaggcag ctcaaatgat
     9541 gggaatgcgg caggtagtca aagtatctat agctgaaaat gaaaaaatct tcttcagaat
     9601 ttattcagca tttgataatc cgatgtttca aatagataca ggtatgactt ttactaccta
     9661 tggcgaatgg ggtgcccaaa cagcaccaaa atttctgacc gcaaagtcac aggaaaaaat
     9721 ggtagaactc gtctggcctt gcaacaggac actcaaccgt tggtcgggct ttaatatcga
     9781 acggtctggc gatggaaaaa atttcacccg actcaataaa aaaccattta tcattatgag
     9841 tccagaactt gacattaata tgtattacag agatagtgta agcaattatg tgaagtatta
     9901 ttaccgcatt caggcattgg atccttttgg agatgtcacg gattttagtg agtcagtcag
     9961 ctgttatggt agtgataaaa caccgccagg ccgattagta cttgaagaaa accatggtga
    10021 cggcagtggc attacattga agtggcgttt tgaaaataat caacctgccg ttgatttaga
    10081 tcattttgtt gtgaagacag gtcccggtgt tgattacata catgatactt tgaaaattct
    10141 gcccaactca gctgcctcct ttgagtatcc tgtgagagca aaagtcaaat ctacctactt
    10201 cgaagtagcc gcagtagata cagccggcaa ttattcatcg tccaatccgg tcagatattt
    10261 tattcctgac accgagccac ccaaagcacc tgttgggttc aaagcaacta ttgacactgc
    10321 cggcattgta cgattaagct ggcaactcga cacaacagat gagatacagg gttacagagt
    10381 attcagaaag aatgacccga agcacgaatt tacttgtatg cagcaaggat ttttgaaaga
    10441 aactgtgttt acagatacgc ttgctctcaa tactttaacc aaagacattt attatgcagt
    10501 ggccaccatt gatatgagct ataatatgag caagatgacg gagaccatca agctaataaa
    10561 acctgataaa gctaagccag tacctccaca aatcactcat tacaaactgc gtgacagaag
    10621 cgttacacta caatggaccc aaagcccaag tgatgatgtc gattattacc aattgtatcg
    10681 aacagacgag aaaggtagta cagtgttggt tgctaaaaac ctgagagccg accaattgga
    10741 atatacggat tcagccctga ctgccaaagc cagatatacg tattatctca attcaacaga
    10801 caagtcaggt cttacatctg aaaactcgtt cccactggat attcttgcat ataccaataa
    10861 ccccggaaaa ataattgccc tcacatggtt atcagagaaa gaaaaatttg gattcaaatg
    10921 gacagaccca aaccctaaag ccgcctttta tatcatttac aaagataccg gcagtggcct
    10981 gcaacagtac aaaagtatcg actcggatca aagcaaattc actgaagaaa ccaataaaca
    11041 aactaaggtt aggttcggcc tacaggcagt ttatcaaaat cagcaaaagt caggcattta
    11101 ttcactcgaa tggtataaac ctgaacattg aataatatta attaatattt taaatatcga
    11161 attatgaaaa atgtattatt gttttgcgcc ttgtgtcttc tacacccatc aatattctca
    11221 cagcagatat ttttcgagga ttttgatacg tacaccttgg gtcctatagg tgatcaaaat
    11281 ccagaatggg ttaatctttc gaatgctcag gtaaatgtta aaattgatgg gggcaatttt
    11341 gtaaattgct caggctctga taatcaaaat gcgtatctga gttttgaatc agctgacttt
    11401 gaagagtgca aaagaatagt ttctactagc tattctaatt ttgtccttga catgaaggct
    11461 actttgaatt caggtattag cgtaaaaatt gtggatacag acgattttgc tttcgaatac
    11521 ctttatgagt ttgacggttc tgggaacttt gaatttgcat tcatactaga ttttgtatat
    11581 caccagattc aggtaatcag gaatggagta acagtaaaaa cagataactt acccgctggt
    11641 tttaactcta ttgatgagat tcactttaat aattccaccg gcaaatttaa actaggctgt
    11701 gttgaactgt ctgatgtcac agatgtagat ggtgatggtt atctggcatc aaatgactgt
    11761 gatgacaatg atgccaacat caatcctggt aaggcagaaa tagcctacaa tggcaaagat
    11821 gacgactgca atcctgccac actcgacgat gacctcgacc aggacggtta cccaaaggca
    11881 accgactgca acgatgacga tgccgacatc aaccctggta aggcagaaat agcctacaat
    11941 ggcaaagatg acgactgcaa tcctgccann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnat gacctcgacc aggacggtta cccaaaggca accgactgca acgatgacga
    12121 tgctaatatc aaccccggag cagcagaaat acccggaaat agcattgatg aaaattgtga
    12181 cggaatatta ttggcagagt ttcatcagag taccaaactt tttgtagaat taatcccaaa
    12241 cccgaccaga gattaccttg aaataaaatc cagttcaaaa attgattatg ttaaaattta
    12301 taacattgcc gggtcagagg tgagttctta caaaataaat gaatcaaaca taatttatct
    12361 aaaagatata agagagggca tctatttcat acatgcattt tcatccgaat ctagtggatc
    12421 ttatattgga aaatttgttg taaattattg agtaagtaaa gtataaacaa ttaaaccaaa
    12481 ataatgcatt tggaattgtt agcgaaaaca atttactacg aaaaattggt ctgcacttca
    12541 acaaagcata gtgaccctgg ttttggagct tttgaaatgg atttgaagta ataattattt
    12601 tcaatatata gtaaattacg gtattcgagt taaactacaa aactttttcg ttagtataaa
    12661 tgtgtttcta agtgctgtct ttttgatatt taaatttgat attagacggc acttttttta
    12721 ttaagattaa tacattaaca ttgtatctga aattttatgt aaatataatg tatgtttgag
    12781 aattgaatgt tttgaaaaag ttaaccatcc ttatttattt cttttcgttt gtggtttgga
    12841 atttgaaggg tcagaccttg agtttcaaaa cctataatag taattcaggt ctttctcaga
    12901 atagtattta ctccattaca caggataaag aaggattttt atggtttggc tcacaggcag
    12961 ggctcaaccg ctttgacgga aatgagttca aactattcgt tcccagaata gtgaccaatc
    13021 ataaggtcat gactgatttt tccaagatga taactgccct tcacagagat gctaccaatt
    13081 ttctgtgggt gggcacaacc aatgaactcc tgatttacga tgcagttaaa aataaatggt
    13141 acacaccgga ggaaaaatat cctggatttt atattgcacc tctggcttgg gttgtagctg
    13201 ttaccgaaga tgctggcggc aaactatggg tcagcacccg taaggcagga ttgtattgct
    13261 acgacaaaat caaaaagaag atgattgtcc tgccaaagca aatagtacag gcaaaaccgg
    13321 aaattaaaca ttgtcgcttt gataatgatc atatatgtac ggccagagat aatgttatct
    13381 atttgttgaa tgatacttcg gtgcagttaa ttaatacaga caagcttctc caatcaaatg
    13441 gtaatattgt aaccgcagtc gaatcggtca atggtgatgt ttggttcatt gccaacggca
    13501 aatcaattta ttcatataat cttgggagca gtaagttgac tcgccacacc ttaaattttg
    13561 acaacaagca atatcttaaa gatttaagca gcctttattt ctcagatgac aataccttat
    13621 ggataagctc aagaacatca gggctggtca ggtacaatat tattacttca gaagcagatt
    13681 atgccaaggc tggtaatgag gagaatgcat tgaagaagaa ttttatttta agcatttttc
    13741 aatcagaaga cggcctaata tgggtaggta caagcggagg tggcgtgtca aaatgtgaag
    13801 taaataaagc cggctttcag ctatatcatg tagaaaataa gggtagtccg gaagagagtt
    13861 atgacaatat gatactaagt gcttacacgg atgatgaaac ggagttttat atgggtacaa
    13921 gctacgccgg cttattaaaa tacaatatta gaacaaaaca atttcactac tttgcaccat
    13981 ttaccagttc taataatgtg ccacagtcta acaacattta ttcagtcatc aaaggtgata
    14041 aaaccttgct ttggcttgct acctggggcg gattgtatag ttttgacaaa ctcacgcaca
    14101 gatttaaagg ctattttgac aaaaaaaatg aatttacgcg agagctcaat tgtgccatca
    14161 aacttaagtt aagtaacaaa atcctgattt gtagctcaaa aggcattcct ttattgtttg
    14221 ataccgacaa aaagat
//
LOCUS       DAOQQY010000037        14236 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1394_length_14236_cov_5.843664, whole genome shotgun sequence.
ACCESSION   DAOQQY010000037 DAOQQY010000000
VERSION     DAOQQY010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14236
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1394_length_14236_cov_5.843664"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="PLE23_03510"
     CDS             complement(<1..491)
                     /locus_tag="PLE23_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMX82364.1"
                     /translation="MKEHMRPESLMMSYGYKPELSEGAVKCPIFQTSTFVFKTAEEGK
                     AFFEIAYGIREKLPNEELGLIYSRLNNPDLEILEDRLCLWDGAEDCAVFESGMSAITT
                     VLLEFLKPGDLLVYSNPLYGGTDHFINHFLPKLGIHTLGFKPGEERNGLADLIKGSPH
                     ADKL"
     gene            complement(643..4452)
                     /locus_tag="PLE23_03515"
     CDS             complement(643..4452)
                     /locus_tag="PLE23_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82365.1"
                     /translation="MGFCIESYATHIIGGEITYSCLGNNKYEITLVVYRDCFNGVPPF
                     DDTVSIGVFNYKNELIDDIRIPYMHDDTIKQTLIGDCKVIPPNVCVHTSVYKDTIDLK
                     PILGGYTLVYQRCCRNKTIVNIVKPDDTGASFVIQITEKALLECNSSAKFKYWPPIYI
                     CANEPIIFDHSAIDPDGDSIVYKLCTPTNGLKDDPQPQPPNAPPYDKVVWKDPPYNID
                     NLMGGVPLAIDSKTGLLTGIPNTIGQFVVGVCIEEYRDGKIISTTTRDFQYNVGLCGK
                     TESAFFVPDVNCSGLNVSFINQSQNGNKFEWDFGDPTTTLDKSTIFNPNYTYPDTGTY
                     TVKLIAKASEICTDTFFKTIKVYKNSLSPEYKLKLLSCTDSMVVAVTDASIDTVSSIV
                     SWKYYLKIGGVLKDSSTLKNPVFVGDTLGIWEITLIVTNELGCEKVRKRNIVFPKMIQ
                     FKFPTDTLYKCPGQELILPGPVDTNYILTWAPGSYLSDSTKANPVCFTPQDIHYTLKV
                     KDKLSICNVTRDLWVKVAPEFSINLPDSISTCDKDFKLTISTDAYLPKIYTSYQKDFS
                     QIIDTTDEVMLNSISGQKLIFVKVTDFYGCEKIDSVKITGNGIYHENPDDIVICPGDT
                     ANVVVKQGIGPTMNIQWSADPSLNANGFGAKIFPAQSGKHWYYYTLSNQAGCLVADSF
                     AIFVVDTVPTVFFTYHQCAGTRIFVEANGKNAPYYHWLVDDGDSVQYFQSAPFSIDFA
                     DTGVYQIKAWVPGAVCLDTLLGQISVGKGSLDLSYQLDVPVCHDSLVVALKNTSEGYD
                     PNIHKAYWYSNFGGLVEGDSLKITLHKVQPDLKILLIIDQMNGCVDTIPANISGTFIP
                     TIPDDSIKTCSGREIKLNYNAYAYLSFEWQPAALVSDPTIASPIIKSSQSEVLTASIK
                     AQITDSVFCQSAFKVFLDVLPSAEYTVQYDSVICDSASIISVKTDIGNSILWFDTEKL
                     DNVIAIGDSLKVKPDRHSTYYGTVTAPSGCVDTIMVNILNQLPQILPNPAILCAGDTA
                     IVSVINLFAGDQLSCVFDPSGLVFVDSATGNYLVTADKSFELDVTCTNQYGCSVTKKL
                     PVDVFEYQPPLNITADPTILATGQSAQLNATYDPGYSYFWSPAGSLSNAAIHNPVATP
                     EQSTSYTLLIINEDGCRNEASILVQVFNPVCEEPFSFIPNAFSPNGDGKNDRFYIRGE
                     FITEIKLSVYNRWGVLVYYSEDKDEGWDGSYKGDQSSPDVYGYQVEFLCADGKRFFKK
                     GNVSLFR"
     gene            complement(4640..8299)
                     /locus_tag="PLE23_03520"
     CDS             complement(4640..8299)
                     /locus_tag="PLE23_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82366.1"
                     /translation="MRRFFKYFLILLGIALYSEKANATHIVGGELYYQCLGNDQYRIT
                     LNVYRDCYNGVPPFDDPAWVGVFDASGNLLDTIAMPFIGKSDTLLNDDICLYVPPSVC
                     VERYSYTTVVTLPYIAGGYTLAYQRCCRNQTIVNIINPSQTGATYTIRITEDALKDCN
                     SSPQFKGWPPIFICRGKPIIFDHSAYDSDGDSLVYVFCTPFVGGIYNVKNQPIPPFAP
                     PYDTVVWKKPYSNANLLGGTDPLKVDHFTGLITGTPPTLGQFVVGIRVEEYRNGKLYS
                     MVKRDFQYNVVECGKIEALWLPESFNVCDSLAVQLENNSTMATDFKWFYRNINKPGSK
                     DELFSTEKNPKLIVPEYGSYAVKLVAEPLSSCSDTLEQIIHFKKNTLHADFGYQVIDC
                     IDSITIQLLDQSHDSTDTVVKWDWQVTNNMNADTLNFTEQNPKFTLHQSSKVKVRLIA
                     ETQFGCNDTIIKEFQANILQLDSLLDTVKICKLDTVNLNPKYFKDYSYTWSPVTGLIP
                     PVDSIPNPWASPLTTTTYTMVYTDSTNLCHVSQDITVLVLRSIDSLDFDVSIPYCQDS
                     INIAITNVQVYSATGTGQVSWYWELTSPHGTQTSTDKTPIFSIPGSGWVTIKVIATTE
                     DSCSYEKTKSFQANTIPKNPAADTVRICIDECVHLYPGADTMLIYDWVPPGGLDDPTS
                     PNPLACPDSSTIYFVNYTDTVGLCIVHDSVLVMVNDTMVDFEFDIRCNGLTVQFINTS
                     TPGIIHFFWEFGDPANSKSQDKDPVFTYPGPGTYKVLLYTTDDWVCRDSIEKIIVLDS
                     LPNKADFDYQYEECENYALVHFFDKSTSKYAKIIKWTWTVDGVLVSNEQNPTIKFDKV
                     GKYKVCLIIETDEGGCMDTLCKIIDINYINNTCIQQINACLEEYVPLNPGGDSTYTYE
                     WFPCENLSDCNGVNPIATYPFTSDYYTVKISLLLPNGDTCITYDTITIIKDFVYGEVM
                     NDTTICKDSITLHAKNLINVATIEWYKVPAIFLGMGNDLPIKVTESGKYYAVFTSPLG
                     CKHTDTLMVTKLSGPIPQIDATPLSFCEPTAIQLTTGANPSYTYQWSPPELFTNPTIF
                     NPTSLPIDKTTIFTVVVNDTLGCEGSASITVTHECPICDEPFIFVPNAFSPNADGKND
                     VLYVRGESVIDGMDFVIYNRWGQEVFRSKDLNTGWDGTFNGKELSPDVYGYYLKARCF
                     DGTIYTKQGNINLLR"
     gene            complement(8363..10813)
                     /locus_tag="PLE23_03525"
     CDS             complement(8363..10813)
                     /locus_tag="PLE23_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82367.1"
                     /translation="MKKYFYILIAALLTAGISNAQTARNFEKAADKAMADKDYYSALE
                     YYQTALDIKPTDIGLMYKVAESARGFNAYKLAEDHYKKVLESPEAINYPEARFWLGQM
                     QIFQGNYNDARTNLEAYAHDEKTTKEMAAKAHLCLGTCEFVTARSVPMQGNAQITKLD
                     ETVNSPWNDFGATVLDGKLYFTSMKFESPQDKKVPARSFAGVLGQDKGQLPKRLNIPG
                     TEKALHFAHSTFNHSKDKVYFNICTYLNSSDIRCDLYVSQVMADGWSEPKLLPEPVNM
                     AGYTTTQPYLSYDPASRSERLFFVSDRPGGKGNYDIWYIDIDANGDFGTLMNLESVNT
                     PENEITPFYQASNKALYFSSDGYAGFGGYDVFTSHWTGIKFSTPENIGSPVNGSRNDL
                     YYWLNEKEDTAYFASNRDESTFIVKEKNACCNDIYLTRYIIINLDALTFESATQAPLN
                     ACTVKLYEVTPESRVLVAELTNPQSNLSKFRLNPGRDYWVVASREGYEPDSALVTTKG
                     VGDSKDMTQKLYLKPSTIDLEIFTFDDATKKALENCKVQLFDITDTDKNDILVYENVN
                     PLSNNFTAKIRPCRKYRLIVTRNGYVTHTEEFGTTCDGSQKSMKKDVYLRKATLEDYL
                     PLNVYFENDQPVAKSKSVTTVLEYLTTYNTYYAKKEMYADNYLPQFKSADRAQVEQKV
                     FDFFDNRLKPGKDSLEAFTRILLDLLKNGDKIYLELQGYTSPLASLEYNQNLGSRRVN
                     SLINHFRSYDSGVFLPYLKSGQLKLTKRSYGETRASRKVSDSRKDLSASIYSPEASYE
                     RRAAIIDVTRAGKMKKKK"
     gene            complement(10859..11899)
                     /locus_tag="PLE23_03530"
     CDS             complement(10859..11899)
                     /locus_tag="PLE23_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMX82368.1"
                     /translation="MKITLRCVIPLVVLLFCLTPTVIKSQDSDLHFTQYGMSPINLNP
                     GLTGIFNGDIRYTGNYRSQWASVPVGYSTASFAYDQKIYNAKLKNGLFGVGGIFNYDQ
                     AGASALRSLQLMLAGSYTQRISQRNFLTLGASAGVAQRAFKDGDLTWDEQYIGNQYNP
                     NNPITESFASKAKIYPDFGLGINWHFQVPNSKRTQFDLGASLYHLHSPNTSFYDEQDV
                     QIKPRLSIQLGSSFKILPKFDVIINANYYDQGANNQTIAGGGLRYHFDDRIGREMALQ
                     VGGEYRFLFKDAWAPVVTFYYHQWKVGVSYDVNVSKFDRATRNQGGLEVGIMYIVTKV
                     KPLPVFKSCPIF"
     gene            12109..13629
                     /locus_tag="PLE23_03535"
     CDS             12109..13629
                     /locus_tag="PLE23_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMX82369.1"
                     /translation="MKIFNAEQIKKCDSVTCENQNIQIRELINRAGNSFVEMLTATDA
                     DLSGKIFVICGYGNNGADGIAIAKLLYARYCDIELLLIPAESKKLNSEYMQFYLDNPV
                     DSQPFPVHIIKKINQLNDLEKPDLIIDAILGTGTTRAVEGIIDEVISWMNTENAKVVS
                     VDIPSGICADKILDGNAVEAELTITFQFPKFTFFLPEYAKNTGRWMVADIGCDQDFLH
                     NEPAEYHWIDKQLVLQHLKVRDRFAHKGNFGHAYIISGSYGKLGATIMCSKAVLRTGS
                     GLATIHAPACAYEILQMAIPEAMVDVDRHKYFISEVNIPARCTAVAIGPGIGTQHHTQ
                     RMLDELFHKNITIPVVLDADALNLMSMYPEIIRNLPKESILTPHVKEFERLFGPTATY
                     SDQIKLQKQKSAELNAYIILKGGYTTLSCPDGEVYFNTNGNPGMATGGSGDVLTGIIT
                     GLAAQGYSRKEACIIGIFLHGMSGDLAVQGGQSYESLLASDLIDNIGHTFRKIRQS"
     gene            13626..>14236
                     /locus_tag="PLE23_03540"
     CDS             13626..>14236
                     /locus_tag="PLE23_03540"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HMX82370.1"
                     /translation="MKKLVVLTGAGISAESGLKTFRDAGGLWEGYRIEEVASPDGWRT
                     NQDKVLLFYNQRRHQLKTVVPNKAHKTLAELERAFEVQIITQNVDDLHERAGSNNVLH
                     LHGELLSVRSEKNAALRYRWNDDLNKGQLAEDGAQLRPDIVWFGEEVPLLSNAAEICS
                     TADYLLIIGTSLQVYPAAGLVYEIPPHCPVIYIDPKADSNFTQ"
BASE COUNT         4117 a         2957 c         2903 g         4259 t
ORIGIN      
        1 agtttgtccg catgtggtga accttttatc aaatctgcca atccatttct ttcttcacct
       61 ggcttaaatc cgagcgtatg gattcctaac tttggtaaaa aatgattgat aaaatgatct
      121 gtaccgccat agagcggatt gctatataca agcagatcac cgggtttcag gaattcgagc
      181 aaaactgtcg taatggccga cattccgctt tcgaatactg cacaatcttc ggcaccatcc
      241 cataagcaaa ggcggtcttc gagtatctca aggtcagggt tattcaatcg actgtatata
      301 agccccagct cttcgtttgg gagtttttca cggataccat aagcaatttc aaaaaaagct
      361 tttccttcct ctgccgtttt aaatacaaag gtagatgttt gaaaaatcgg acattttacc
      421 gcaccttcag atagctctgg tttataaccg taactcatca tcagactctc tggccgcatg
      481 tgttctttca taaaataaaa aatttgtgta ttataaaaat gtgttagtat gttctacgtc
      541 aattattcaa taaaagatta ataaatttac attcatcgag gaggcttcat ttcaatgtcg
      601 tgatttctta ctacttcaac ctccgatcaa tccggttttt atttatctga ataagcttac
      661 attgcctttt ttgaaaaatc tctttccatc ggcacataga aattcaacct gatagccata
      721 gacatccggt gatgactggt cgcctttgta acttccgtcc catccttcat ccttgtcttc
      781 agaataatat accaatactc cccatctatt gtaaacactc agttttattt cagtaataaa
      841 ttcgccccgt atataaaacc ggtcgttctt accgtctcca ttcggagaaa atgcattcgg
      901 aataaaacta aaaggctcct cacataccgg attgaaaact tgtaccagta tgcttgcttc
      961 attcctacaa ccgtcttcat tgataatcaa caaagtataa ctcgtggact gctcaggtgt
     1021 agccacagga ttatgaatag cagcattgga caaactacct gcaggtgacc agaaataact
     1081 ataccccggg tcataggttg cattcagctg agccgactgg cccgtagcaa gaattgttgg
     1141 gtcagcagtg atattcagtg gtggctggta ttcgaataca tcaaccggga gcttttttgt
     1201 taccgaacat ccatattgat tggtacatgt aacatcaagt tcgaaactct tatcagccgt
     1261 caccagataa tttcccgtag cagaatctac gaataccaat cccgaagggt caaaaacaca
     1321 acttaattga tctccagcaa acagattaat aacactaacg atagcagtat ctcctgcaca
     1381 cagtatggca gggttaggga gtatctgtgg caattgattg agtatgttca ccataattgt
     1441 gtcaacacag cccgatggtg cggttactgt cccgtagtat gtactatgtc tgtccggctt
     1501 tactttcagg ctgtctccaa tggctattac attatccagt ttttctgtat caaaccagag
     1561 aatactgttg ccaatatcag tttttacaga aatgattgag gccgaatcac aaatgactga
     1621 atcatactga accgtgtatt ctgccgaagg gagtacatca agaaatacct tgaaggccga
     1681 ttgacaaaac accgaatctg ttatttgcgc cttaatactg gcggtcagaa cttcactttg
     1741 gctactcttg atgataggtg aagcaatagt aggatcagaa accaatgctg cgggctgcca
     1801 ctcaaaactg aggtatgcat acgcattata attcagtttg atttctctac ctgaacaagt
     1861 cttaatactg tcatcaggta tggttgggat gaaagtgcct gaaatgttgg ccgggattgt
     1921 atcaacacaa ccattcatct ggtctattat taacaatatc ttgaggtcgg gttgtacctt
     1981 atgcaatgta attttcaacg aatcgccttc taccagtcct ccaaaatttg aataccaata
     2041 ggccttatgt atattcgggt catatccttc gctggtattt tttagagcca caacgaggct
     2101 atcatgacaa acaggcacat caagctgata actcagatcc aggcttcctt tccctacaga
     2161 tatttgaccc aacaatgtat cgagacacac agcacccgga acccaggctt taatttggta
     2221 aactcctgta tcagcaaaat cgatcgaaaa tggtgctgac tgaaagtatt gtacactatc
     2281 accatcatca accaaccaat gatagtaggg tgcattttta ccgttcgcct ctacaaatat
     2341 cctcgtgccg gcgcattgat ggtatgtgaa aaaaactgtc ggcacagtat caacaacaaa
     2401 tattgcaaat gaatcagcaa ccaaacaacc tgcctggtta ctcagcgtat aataatacca
     2461 atgttttcct gactgtgctg gaaaaatctt agcaccgaat ccattggcat ttagggaagg
     2521 gtctgctgac cattggatat tcattgtcgg gcctataccc tgcttgacaa ctacattggc
     2581 ggtatcaccc gggcatataa caatatcatc aggattttcg tgataaatac cattgccggt
     2641 tatcttaacc gaatcaatct tctcacaacc ataaaaatct gttactttta caaaaataag
     2701 cttctggccg gatatggaat tcagcatgac ttcgtcggtt gtgtcaataa tctgtgaaaa
     2761 atctttttga taactagtat atattttagg caaataagca tcggtgctta ttgtaagttt
     2821 aaaatcttta tcacatgtag atatactgtc tggtaagttg atggagaatt caggagccac
     2881 tttaacccaa agatcgcgcg taacattgca gatgctaagc ttgtctttta ctttgagtgt
     2941 gtaatgtata tcctgtggtg taaaacacac cggattggct ttggtgctat cagacaaata
     3001 actgccgggt gcccaggtca aaatataatt cgtgtcaacc ggccccggaa gaataagctc
     3061 ctgcccagga catttgtaaa gtgtgtcggt cggaaatttg aattgtatca ttttggggaa
     3121 tacgatgtta cgtttcctga ctttttcaca accaagctca ttcgtaacga tcagcgttat
     3181 ttcccatata ccaagtgtgt caccaacgaa aacaggattt ttcagggtcg aactatcttt
     3241 tagtacaccg ccgattttga gataatattt ccacgacaca atgctactca ccgtatcaat
     3301 tgaagcatcg gttactgcca ctaccatcga atcggtacaa gacagcagtt tgagcttgta
     3361 ctcaggtgac agtgaatttt tataaacttt aatggtttta aaaaaagtat ctgtacatat
     3421 ttcacttgcc ttcgcaatca acttaacagt ataagtacct gtgtccgggt aagtataatt
     3481 tggattaaaa atagttgatt tgtcaagagt agttgtcgga tctccaaaat cccattcaaa
     3541 tttattgccg ttctgtgatt gattgataaa gctgacgtta agcccgctgc agttgacatc
     3601 gggcacaaaa aatgcggatt cggtcttacc acacaagcct acgttgtatt gaaaatctct
     3661 ggttgttgtt gaaataattt tcccgtcgcg atattcttca atacagacac ctactacaaa
     3721 ttgccctatg gtattgggta tgccggtgag taagccggtt ttgctgtcaa tggccaatgg
     3781 cacacccccc atcagattat cgatattgta aggtggatcc ttccagacta ctttatcata
     3841 aggcggggcg ttgggtggct gaggttgtgg gtcatctttt agtccgttgg taggtgtaca
     3901 aagtttataa acaatagagt ccccgtccgg atcaatggcc gaatgatcga aaataattgg
     3961 ttcgttggca catatataga taggtggcca gtatttgaac ttggcacttg agttgcattc
     4021 gagcaatgcc ttctctgtta tctgaatgac aaatgatgcc cctgtatcat cgggttttac
     4081 aatattgaca atggtcttgt ttcggcaaca tcgttgatag accaatgtat aaccaccgag
     4141 tataggtttc aagtctattg tatctttgta cactgatgtg tgtacacaaa catttggcgg
     4201 aataactttg cagtcaccaa tcagggtttg cttgatggta tcatcgtgca tgtaaggaat
     4261 tcggatgtca tctatgagtt cgtttttata gttaaaaaca cctatagaaa ctgtatcgtc
     4321 aaagggcgga actccgttaa agcaatctct ataaaccacc agtgtgattt cgtatttatt
     4381 attccccagg cacgaatagg ttatttcacc accgatgatg tgtgtggcat aagattctat
     4441 acagaatccc aaaaagaaca aattcaacca tattatatac ctcatattac taccagcctt
     4501 acttgaattt ttcctacaga aatagaacaa aagaaaaggt ttattcaaat tttttttaaa
     4561 attaatgaaa taggctgtaa aataaaaatg cccgaaacaa aattttattc cgggcattcc
     4621 ttaagtactt ggtatgactt tatctcaata ggttgatatt gccttgcttt gtataaatgg
     4681 ttccgtcaaa acatcttgct ttcagataat agccatatac atcaggcgat aattcttttc
     4741 cgttaaatgt accatcccag ccggtgttca ggtcttttga tctgaatact tcctgacccc
     4801 atctgttgta aatcacaaag tccataccgt caataacact ctcaccacgc acgtagagta
     4861 catcattttt gccatcggca ttcggtgaaa atgcgtttgg cacaaagatg aatggttcat
     4921 cacatatcgg gcattcgtgt gtgactgtaa tggaagcaga accttcgcac ccaagcgtgt
     4981 cgttgactac tactgtaaat atggttgtct tatcaatcgg taagctggtt gggttaaata
     5041 tagtaggatt agtaaataac tcaggtggcg accattgata agtatagctc ggattggctc
     5101 cggttgtcag ttgtatcgct gtcggttcgc agaatgagag tggtgtggcg tcgatttgtg
     5161 gtattggccc tgaaagcttg gtaaccatca aggtatctgt atgtttacaa ccgagtggtg
     5221 atgtaaaaac agcatagtac tttccactct ccgtcacttt tatcggtaag tcattaccca
     5281 taccaaggaa tatcgcaggt accttgtacc actcaatagt agctacgttt atcaaattct
     5341 tggcatgtag ggtgattgag tccttacaaa tcgtagtatc attcattact tcgccataca
     5401 caaaatcttt aataatagtg atggtgtcat aagtaatgca tgtatcgccg ttaggtaata
     5461 acaggctgat ttttacagta taataatctg atgtgaacgg ataagtcgct atcggattta
     5521 caccattgca gtcagagaga ttctcgcaag ggaaccattc gtaggtgtag gtcgagtctc
     5581 ctcccggatt gagcggcaca tactcttcaa gacaagcatt gatctgttgg atacaggtat
     5641 tgttaatata attgatgtca ataatcttgc aaagcgtatc catacatccg ccttcatctg
     5701 tctcaatgat cagacagact ttgtacttac ctactttatc aaacttgatg gttgggttct
     5761 gttcattgct tactaaaaca ccatcaacag tccatgtcca ttttatgatt tttgcgtatt
     5821 tgcttgttga cttatcgaag aaatgaacaa gtgcataatt ttcacattcc tcatattgat
     5881 agtcaaagtc tgccttatta ggtaatgaat cgagtactat gatcttttct attgagtcac
     5941 ggcaaaccca atcatccgtt gtataaagca aaaccttgta tgtaccgggg cccggatagg
     6001 taaacaccgg atctttatcc tgagattttg aattggccgg atcgccgaat tcccagaaga
     6061 aatggatgat acctggtgtg gatgtattaa taaactgtac agttaaacca ttacagcgga
     6121 tgtcaaattc aaaatctacc atagtgtcgt tgaccattac aagtacagaa tcatgtacaa
     6181 tacaaagacc tactgtgtct gtataattga caaaataaat agtactactg tccggacagg
     6241 caagcggatt tggcgaagtt gggtcatcaa gtcctcccgg aggtacccaa tcatagatca
     6301 gcatggtatc agcacccgga tacagatgta cgcattcatc tatacatatc ctgaccgtat
     6361 cagctgccgg atttttcggg atggtattgg cttggaatga ttttgttttt tcatacgaac
     6421 aagaatcttc agtggtggca attactttaa tagttaccca gccagatccc gggatagaga
     6481 aaatcggtgt tttgtcagtt gaagtctgtg ttccatgcgg agaagtcaac tcccagtacc
     6541 agctaacctg tccagtaccc gttgcagaat aaacctgaac gttagtgatg gcaatattga
     6601 tactgtcttg gcaataaggt atggacacat cgaaatcaag actgtcaata ctcctcagca
     6661 caagcacagt aatatcttgc gaaacatgac aaagattagt agagtctgta taaaccattg
     6721 tgtatgtggt agtagtcaat ggtgatgccc atggattggg tatggagtca accggcggaa
     6781 taagccctgt aacaggtgac caggtataac tgtaatcttt aaaatattta ggattgaggt
     6841 taacggtatc caatttacag atttttacag tatccaacaa cgaatcgagt tgtaggatat
     6901 tcgcctgaaa ctccttgatg attgtatcat tacaaccaaa ctgtgtctct gctatcaacc
     6961 taacctttac cttggagctt tggtgaagcg taaatttagg attttgttct gtaaagttaa
     7021 gggtatcagc attcatgtta ttagtcacct gccagtccca ttttactact gtatctgttg
     7081 aatcatgcga ctgatcaagt aattggatgg tgatggagtc aatacaatca atcacctggt
     7141 agccaaaatc ggcatgaagt gtgtttttct tgaaatgaat gatttgttcg agtgtatctg
     7201 aacaggatga caatggttcg gccacgagtt taacagcgta gcttccatat tccggtacga
     7261 tcagtttggg atttttttcg gtagaaaata attcatcctt actgcccggt ttattaatat
     7321 tcctgtaaaa ccatttaaaa tctgtagcca tggtgctgtt gttctcgagc tgcacagcca
     7381 gactgtcaca tacgttaaac gattcaggaa gccacaaagc ttcgatctta ccacattcta
     7441 ctacattata ttgaaaatct ctcttcacca tgctatacag cttaccattc ctgtattctt
     7501 ctactcggat acccacaaca aactgcccta aggtaggcgg agtaccagta ataagtcccg
     7561 taaaatgatc tactttaagc ggatcagtac cgccaagcag attagcattt gaataaggtt
     7621 ttttccacac aacagtatca taaggtggtg caaacggtgg aatcggttga ttttttacat
     7681 tataaattcc tcctacgaat ggtgtacaga atacatatac caacgagtcc ccgtccgaat
     7741 cataagcact gtgatcaaaa ataatcggct tgccccggca gataaatatc ggaggccagc
     7801 ccttaaactg tggtgagctg ttacagtctt tcaatgcatc ttctgttatc cgaatggtat
     7861 aggtagcacc ggtttgtgat ggattgatga tattgacaat cgtctggtta cgacaacacc
     7921 tttggtacgc aagtgtatat ccaccagcta tatatggcaa tgttactaca gttgtatatg
     7981 agtaccgttc cacacatacc gaaggcggca catatagaca aatatcatcg ttgagcaatg
     8041 tgtcagattt accaataaaa ggcatagcaa tggtatcaag aagattgccc gaagcatcaa
     8101 aaactcctac ccatgcagga tcatcgaatg gcggaacacc attataacag tctctgtaca
     8161 cattaagtgt tatccggtac tggtcattgc ccaggcattg ataatacaac tcaccaccta
     8221 cgatgtgtgt cgcatttgct ttttcagaat agagcgctat tcctaatagt atcaggaaat
     8281 atttgaaaaa tcttctcatt cctgtatcat ataatttttc caaacttttc ataactgcga
     8341 tatgatatca tttgatataa tattatttct tcttcttcat tttacctgcc cgggtcacat
     8401 cgataatggc tgctcttcgc tcataactcg cttcaggcga atatattgat gcactcaggt
     8461 cttttcggct gtcactaacc tttctgctag ccctggtctc tccgtaactg cgtttagtaa
     8521 gtttgagctg ccctgatttg agataaggca ggaatacgcc tgaatcataa gatcggaagt
     8581 ggttgatcaa gctgttaacc cttcttgacc caagattctg attgtattct aaagaagcca
     8641 acggacttgt gtatccttgt agttcaagat aaatcttatc tccattcttc agaaggtcaa
     8701 gtaatattct ggtaaatgcc tccagactat ctttacccgg ttttaatctg ttgtcgaaaa
     8761 agtcaaacac cttctgttct acctgtgctc tgtcagctga tttgaattgt ggcaggtagt
     8821 tgtcggcgta catttccttc ttcgcataat aagtattgta tgtagtcaaa tattccaaca
     8881 cggttgttac tgacttcgat tttgcaaccg gctggtcgtt ttcgaagtaa acattcagag
     8941 gcaaataatc ttccagtgtt gcttttctga gataaacatc cttcttcata ctcttctgtg
     9001 atccatcaca ggtagtacca aactcttcag tatgcgttac ataaccattc ctggtaacga
     9061 ttaacctgta ctttctgcac gggcgtattt ttgccgtaaa attattcgaa agcggattga
     9121 cattttcata cacaagtata tcgttcttgt ctgtatctgt tatatcgaat agttgtactt
     9181 tacagttttc gagtgctttt tttgttgcgt catcaaatgt aaatatctca agatcaatgg
     9241 ttgatggttt aagatataat ttctgggtca tatcttttga gtcaccaaca cctttggtgg
     9301 tcaccagggc agaatcgggt tcgtagcctt cccgagaagc gaccacccag taatcgcgac
     9361 ctggatttag cctgaatttg gacaaattac tttgcgggtt ggtaagttct gctacaagca
     9421 ccctactttc aggcgtcact tcgtaaagct taacagtaca agcattgagc ggagcctggg
     9481 ttgcagattc gaatgtgagt gcatctaggt taataataat gtaccgggta aggtaaatgt
     9541 cgttacagca agcatttttt tcctttacaa taaatgttga ttcgtcgcgg tttgaagcga
     9601 agtatgctgt atcttccttc tcgttgagcc agtaataaag atcattacgg ctgccattga
     9661 ccggcgaacc aatattttca ggagtactga acttgatacc agtccagtgt gaagtgaaca
     9721 catcataccc accgaaaccg gcataaccgt cggatgagaa atacaatgcc ttgtttgaag
     9781 cctgataaaa cggagtgatt tcgttttccg gggtattgac gctttccaga ttcatgagtg
     9841 taccaaagtc accattggca tcaatgtcta tataccaaat gtcataattg cccttaccac
     9901 ccggtctgtc agagacaaag aataatcttt ctgacctgga agccggatca taagacaaat
     9961 aaggctgagt ggtggtatag cccgccatat tcaccggctc gggaagtaat tttggttcac
    10021 tccaaccatc ggccattacc tggctgacat acaggtcgca tctgatgtca ctgctattaa
    10081 gataagtgca gatattaaaa tatactttgt ctttactatg gttaaatgta ctatgtgcaa
    10141 agtgaagtgc tttttcagta cctggaatat tgagtcgttt tggaagctga cctttatcct
    10201 gccccaatac acctgcgaag ctccgtgcag gcaccttttt gtcttgaggt gactcgaact
    10261 tcatgcttgt gaagtacaat ttaccatcca ggacagtagc gccaaaatca ttccaaggag
    10321 agttgactgt ttcgtcaagt ttggtgattt gggcatttcc ttgcataggg acgcttcggg
    10381 cagttacaaa ctcacatgta cccaaacaca ggtgggcttt ggcggccatt tccttggtag
    10441 ttttttcgtc atgcgcgtag gcctccaggt ttgttcttgc gtcattataa tttccttgga
    10501 atatctgcat ttggccaagc cagaaccttg cttccggata gtttatagct tcagggctct
    10561 cgagtacttt tttgtaatgg tcttcggcga gcttgtatgc attaaaccct cttgcagatt
    10621 ctgccacctt gtacataaga ccgatatcgg tcggttttat atccagagcg gtctgataat
    10681 attcgagtgc actgtaatag tctttatctg ccattgcttt gtcggcggct ttttcaaaat
    10741 ttctggcagt ttgtgcgtta gaaattccgg ctgttaacag agctgctatc aatatgtaga
    10801 aatatttttt catttctcct gatatttaat tggaatggat acggttaata gaattttatc
    10861 agaaaattgg gcatgatttg aatactggca atggttttac cttagtgacg atatacatga
    10921 taccaacttc caagccaccc tgatttcggg tagcacggtc aaatttcgat acattgacat
    10981 cataactgac acctactttc cattgatggt aatagaatgt tactaccggt gcccaggcat
    11041 ctttaaacaa aaatcggtat tcaccaccta cctgcaatgc catctcgcga cctattcggt
    11101 catcaaaatg gtatctgagt ccaccaccgg ctattgtctg attattagct ccctgatcat
    11161 agtaattggc attgatgatt acatcaaatt tgggaagtat tttaaaggaa gagccgagtt
    11221 gaatactaag tcgtggcttt atctgcacat cttgctcgtc gtaaaatgaa gtatttggcg
    11281 aatgcaggtg atacaatgaa gctcccaaat caaactgtgt acgcttggaa ttcggtactt
    11341 gaaaatgcca gttgataccg agaccaaagt caggataaat ctttgctttg ctggcgaaag
    11401 actctgtaat cgggttatta ggattgtatt gattaccaat atactgctcg tcccaggtga
    11461 ggtcaccatc cttgaatgca cgttgtgcaa cacctgcaga tgcgcccagc gtcagaaaat
    11521 tgcgctgcga aatacgctgc gtatatgatc ctgccaacat cagctgcaat gacctgagtg
    11581 ccgatgctcc ggcctggtcg taattgaaga taccgcccac cccgaacaaa ccgtttttga
    11641 gtttggcatt gtagatcttc tgatcataag caaaagatgc agtggaatat ccgacaggta
    11701 cagaggccca ctgacttctg taatttccgg tgtaccgtat gtcaccatta aagattcctg
    11761 tcagtcccgg atttaaatta ataggggaca taccatactg agtaaaatgt aaatcactat
    11821 cttgtgattt aattactgtt ggtgtcaagc aaaaaagcaa aactaccagt gggattacac
    11881 atcgtagtgt tattttcata acacaaataa ttttggttat taaaaaaatt gcaaaatgca
    11941 tttaatttgc aaatatatta aaaacgaact taatatgaga aaatatttcg ggatatatac
    12001 gtgacggata cacgaaggtt actttccgta tagatttttg aaatatgttc agggtttatt
    12061 agttcacttt acaaaaatgc taatgttata attaaaaagc aacatttaat gaaaatattt
    12121 aatgcagaac agataaaaaa gtgtgacagt gtaacttgcg aaaatcagaa tatccagatt
    12181 agggagttga taaaccgtgc cggaaattct tttgtggaga tgcttacagc cactgatgct
    12241 gaccttagcg gcaaaatatt tgttatatgc ggatacggca acaatggagc agacggtata
    12301 gcaatagcca aacttttgta tgcacgatac tgtgatattg agctattgct cattccggca
    12361 gaaagcaaaa aactcaacag tgaatacatg cagttttatc tggataatcc tgttgattct
    12421 cagccttttc ctgtacatat catcaagaaa attaatcagt tgaatgatct tgaaaaacca
    12481 gatctcatca ttgatgccat ccttggtaca ggtacaacac gtgctgttga aggaattata
    12541 gatgaggtca tcagttggat gaacactgaa aatgccaaag tcgtatcagt ggacattccg
    12601 tctggcattt gtgctgacaa aatactggat ggcaatgcag tagaagccga gcttaccatt
    12661 acttttcagt ttccgaaatt cacgtttttc ctaccggaat atgcaaagaa tacgggcaga
    12721 tggatggtag ccgacatagg atgtgaccag gatttcctgc acaacgaacc ggccgaatac
    12781 cattggatag acaaacaact tgtactgcaa catcttaaag tacgcgacag gtttgcacat
    12841 aaaggaaatt ttggccatgc ctacatcatc tcaggcagtt atggaaaact cggtgcaacc
    12901 atcatgtgta gcaaagcggt attgagaaca ggaagcggac tcgcaaccat tcatgcacct
    12961 gcatgcgctt atgaaatctt gcagatggcc attccggaag caatggtaga tgtggacagg
    13021 cacaagtatt tcatttcgga ggtcaatatt cctgccaggt gtactgctgt ggcaatcggc
    13081 cccggtatag gcacacaaca ccatacccag cgtatgcttg atgaactgtt tcacaagaat
    13141 ataaccatac cggtagtatt ggatgctgat gcgctcaacc taatgagcat gtatcctgaa
    13201 attatcagaa atttaccaaa agaaagcatc ctgacaccgc atgttaaaga atttgaaaga
    13261 ttgttcgggc ctactgctac ttattctgac cagattaaac ttcaaaaaca aaaatcggcc
    13321 gaactcaatg catacatcat actcaaaggt gggtacacta ccctttcttg ccctgatggt
    13381 gaagtttatt tcaacacgaa tggcaatccg ggtatggcca ccgggggcag tggcgatgtt
    13441 cttacaggta tcatcaccgg gttggctgct cagggatatt cgcgtaaaga agcttgcatc
    13501 atcggcatct ttttacatgg tatgtcagga gatctggcgg tacaaggagg ccaatcttac
    13561 gaatcattac tcgcatcgga tctgatagat aatattggtc atacattcag aaaaataagg
    13621 caatcatgaa aaaactagtc gtgctaacag gtgcgggcat cagcgcagaa agtggattga
    13681 agaccttcag agatgccggt gggctctggg agggctacag gatagaagaa gtagcatcgc
    13741 ccgatggatg gcggacgaat caggacaaag tgctcctgtt ctataaccaa cggcgccatc
    13801 agttaaaaac cgtagtgccc aataaggcac ataaaacact tgcagagctt gaacgtgcat
    13861 tcgaagtaca gatcatcaca cagaatgtag atgacctgca tgagcgggca ggtagcaata
    13921 atgttctgca cctgcatggc gaactcctaa gtgtgcgttc agaaaagaat gctgcactcc
    13981 gctatcgttg gaatgatgac ctgaacaagg ggcagttggc tgaagatggt gcacagctac
    14041 gaccagatat tgtttggttt ggtgaggaag tgcctttgct tagcaatgcc gctgaaatat
    14101 gcagtactgc cgattatctg cttatcatcg gtacctcctt gcaggtttac ccggctgccg
    14161 gtctggtgta tgaaatacct ccgcattgtc ctgtgatata tatcgacccg aaggctgatt
    14221 caaattttac acaaaa
//
LOCUS       DAOQQY010000038        14113 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1425_length_14113_cov_5.949993, whole genome shotgun sequence.
ACCESSION   DAOQQY010000038 DAOQQY010000000
VERSION     DAOQQY010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14113
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_14113_cov_5.949993"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PLE23_03545"
     CDS             complement(<1..398)
                     /locus_tag="PLE23_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMX82371.1"
                     /translation="MKQLIELSTKTIILKRNEYLKYSGSTDTNLYYLENGSMRIFIVS
                     EGEEQIIRLAYNGNLITSLDSFLTDKPSELFIQAIKNSTVRVITKQQIEQFLKEDENQ
                     KLWIKILENLIVQQLEREIDILTKSPAERY"
     gene            complement(583..3375)
                     /locus_tag="PLE23_03550"
     CDS             complement(583..3375)
                     /locus_tag="PLE23_03550"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82372.1"
                     /translation="MKRIFIPVVFCLLFVSNILANKGGISDGSPCLVCYAPYLFEVAG
                     IKATSATFNYDGYKDQAGVYELEYGLVGFVKGTGTIVSGKKLTATIIGLTPNTEYTAL
                     LRFKCDSGEGSSDLTLNFKTKLTNDVGVSKILSPKSNCELGNDEKLKIQIKNYGGNPQ
                     SLIDVRFSVNQQPISVPPFVDGYYTGVLSTDSTGTFDFDTGFDYSEEGEFKIASWTEL
                     KGDENKLNDTTYYTLFHVPLVKSLPYAVNFEPSQSGWTFNNVRGTTGWEFGTPNTPNI
                     NTAASGNNCFFARIPEGFTDQVEAYLESPCFDFSDAQNEPYLTFNINYDIDTYYHGIW
                     VEYSKDGGLTWERLGQYNDPLKWYNTASNIFGFSTWAGTSMGWTIAGHKLTELKGESN
                     CRIRIAFSTFYNFGGDSGVAVDNITIYNQIDKDLTAVALTNTSTSECGSENDFVKFTY
                     TNTGKKPIVGPNQVKAYYQFENDAVIEEDVPAAVIQVGDSYTYTFKTKFSSYGPGTYK
                     AKAWVQAVNDANAFNDTTSFSLTIPEPTALPLKEDFEKFLLPEGWIGEGYSITAGHNN
                     KTYVIAGNLFTSSSKFSFTTSNIGPLDGGNTLTFDYRYVDYFAGTNPTSADAVKLVLE
                     ISDDCGITFKPLYTIDQSNHIPSADMKNVAVDLSAYDGKIIKLRYRAEYITGDYWVDV
                     DNININGCPLSLGASSKLKNVSFGGTNDGAVTITPSKGTAPYTYLWSNSATTAGISGL
                     TAGNYCVTITDANACKDVDCFDVGNCPQTLGVTGNVTPVSGPGKSDGKIALNTPAGTY
                     SYAWSNGATGKSVSFLKSGKYSVTVTNESGCTQVIDFQINAVATHEIKDLKVLNINPN
                     PTNGLFNVVAVFGKAVSTAIDIYNIYGEPVYSKITGKADQLNVPVDLGDVPAGFYIMK
                     LSADGSTVSRKIIKIQ"
     gene            3707..4603
                     /locus_tag="PLE23_03555"
     CDS             3707..4603
                     /locus_tag="PLE23_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMX82373.1"
                     /translation="MSKYSIKDLERLSGIKAHTIRIWEQRYRLIKPHRTHSNIRYYDE
                     SELKRLLDIALLNKNGYKISKIATFCEHCISHLLSGIATNESDIGQHDELMIIMVTMD
                     EEKFHSTIDSKIKENGFIHVLEYMIVPFLEKINFIRLTGTINTVHENFIINLIRQKIV
                     AATDKIKRKQSGKPGFLLFLPEGESHELILLIMQFLLRSKAYKTYYLGQNTSLYDVEQ
                     AYKLVKPDYIYTILSERFTGQPIGSYIDELNRCCPQSVLLLSGYQLNVLNIPSGNKFK
                     TLNSFAEAISFLDELSKNSISK"
     gene            4713..5462
                     /locus_tag="PLE23_03560"
     CDS             4713..5462
                     /locus_tag="PLE23_03560"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMX82374.1"
                     /translation="MLIAFAGTQACKSDKKADVAATDIANDTLPPNSATKSQIDALPE
                     MKTENTAGTPASTPADAIVFKMANASVSWIGSKGSLKTQTGTISGKSGQIAYKDGQIV
                     SGNVEIDMNKINVTSLAGSDKKDLEDHLKADDFFDVAKYPTAKFEIVRIEPLDSRASR
                     EANMASLVKNTHMMVGNLTIKDVTRSITVPVNIHLAAERIKIATSMFTIERSLWGVKY
                     DLGTVSGKIKEAIVDDKISLQLTFEGMRAAK"
     gene            complement(5784..6413)
                     /locus_tag="PLE23_03565"
     CDS             complement(5784..6413)
                     /locus_tag="PLE23_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMX82375.1"
                     /translation="MVKHIFPTYYWDKPNDKRAIYLTFDDGPIPEVTPWVLDQLAAYD
                     AKATFFCVGDNVRKYPQIFNRLKEEGHKTGNHTFNHLNGWNTPVNEYVENVDQCAELV
                     DSDLFRPPYGKLLRKQSQHLSENYSVVMWDILSGDFDPTITKENCLKNVVNHAVPGSI
                     IVFHDNIKSYDKLQYVLPRVLEYFAQKNYTFSALEFAKSKKAAPALMPA"
     gene            6670..8193
                     /gene="gltX"
                     /locus_tag="PLE23_03570"
     CDS             6670..8193
                     /gene="gltX"
                     /locus_tag="PLE23_03570"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMX82376.1"
                     /translation="MRVRFAPSPTGALHIGGVRTALFNYLLARKHGGQFILRIEDTDQ
                     NRFVPGAENYITEALEWCGLTFDEGPEQGGDYAPYRQSERKPMYRQYAERLVAEGNAY
                     YAFDTAEELEAFRTKDPNFIYNHSTRTLAANSLTLPPDEVTKRIAAGQHYVIRLKVPA
                     EGKVVFNDLIRGEVSFDNSLIDDKVLLKSDGMPTYHLANIVDDHLMKITHVIRGEEWL
                     SSTPHHVLLYRFLGWEDTMPAFSHLPLILKPNGNGKLSKRDGAKFNMPVFPLSWPHHE
                     EENYEGERFTGFREAGFLPEAVINFLALLGWNPGTDQEIFSIEELTEAFSLEKINKSG
                     ARFDFEKAKWFNHQYIQQKSDAELTALIQPDLKARGITADTKFVEKFCSLMKSRINVL
                     TEFATVGYYFFTEELNYDEANIQKKWNAEVAPKLQEVIRYIEKAEMADHQSMEANFKT
                     FLAEAGYKPGEILPLMRLALAGTMQGPGVFEMVELLGRENVILRMNNAVQAFNKIIK"
     gene            8197..8895
                     /locus_tag="PLE23_03575"
     CDS             8197..8895
                     /locus_tag="PLE23_03575"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMX82377.1"
                     /translation="MFFPIGDEQVERGHKPIFSYLFLAVNILVFIFEISLDAESLNAF
                     TFSFGAIPSEFLHGHQLYSLVTSLFLHGGWGHLLGNMLFLWVFADNIEATLGNIRFFI
                     FYIAGGIAATLFHGLLNPASDIPLIGASGAISAVLGAYLVYFPHSRIKVLVLILFSSF
                     RVPALAFLGIWIVNQFIAGYGSLSHVAEESAGVAYWAHIGGFVYGIIIGFYTRRIYIP
                     VLSREAAEQDEGYV"
     gene            complement(8909..9385)
                     /locus_tag="PLE23_03580"
     CDS             complement(8909..9385)
                     /locus_tag="PLE23_03580"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HMX82378.1"
                     /translation="MIPTIRIIHEKRLIGKRLVMSISEYRVGELWQSFMPERKNIQNK
                     LSDDLLSVSVYPSGYFTAFNPANEFDKWAAVEVSDISTLPDGMEKMVIPEGLYAVFQY
                     KGLNTDRSVFQYIYGSWLPASDFILDDRPHFEVLGAKYRNNAPESEEEIWIPVRKK"
     gene            complement(9440..10366)
                     /locus_tag="PLE23_03585"
     CDS             complement(9440..10366)
                     /locus_tag="PLE23_03585"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMX82379.1"
                     /translation="MKKTNFNHLESLYWVFTQVCNDQCVHCYNDSGPNGGRLSDEECL
                     QIISNLPDEIDRIILSGGEPIAKKKQLFLILDALKERYGSRSQIMLQTNGDLLTGEIL
                     DVLIEKGITRFDIASIDRYHKKAGEHLMNLAELFDSRGVDGSDKDPLIDKENYVKPKL
                     SWGYWGATEDMWLGGNWARGRALKNSIWKKDPKHNFCAVLSGGIGFLNGGEDIPQEIS
                     IQLWKINPCCPGTYKAMGDARTEKISQVLARVSASPVFQRIDRGDPLGMGESVGISRA
                     YAMARTEELDNICLWCDEFFKEHFDIEKLDKK"
     gene            complement(10363..11094)
                     /locus_tag="PLE23_03590"
     CDS             complement(10363..11094)
                     /locus_tag="PLE23_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX82380.1"
                     /translation="MWLEVKNISKRFDKNEVLKDISFSLEQGKTLAILGKSGCGKTTL
                     LKIIAGIIPACEGDVFHQHKLITHLPLNKRNIIYLYQDALLFPHLNVFENIAFGLRAR
                     KLGEEEILQKANKLISELGLTGYENQNPQLLSGGQKQRVAFGRSLIVNPDCMLLDEPF
                     SSLDFETRTTMQMFYKEMAEKFNITTVFVTHDLKEALIMGNHFAVMDSGRLTFYKDKS
                     EFINSPETGVMQEKTFWDNINADKT"
     gene            complement(11084..11848)
                     /locus_tag="PLE23_03595"
     CDS             complement(11084..11848)
                     /locus_tag="PLE23_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMX82381.1"
                     /translation="MKTKARIRIIVLINLVMIPFVFLLFLSIAGTWFFPDVLPGHWSL
                     NAWHGLFLGNDFWWVLLKSVVLSSSIAILATAMAFFISRQLSRHRYRDILHSIAYLPY
                     SFSPVIYAFCIQFLFIRSGLKGTYAGVLIAQLLISFPFVVLLFSKHWNHELKNQEILS
                     MSLGASEYQTVVRVTLPLTMRLIKLSLIQTFLISWFDYGISLVIGRGIAQTAILRIYQ
                     YIAEANIAYAATASLVLLIPPVMLSIVQHKLSSNVA"
     gene            complement(11845..12687)
                     /locus_tag="PLE23_03600"
     CDS             complement(11845..12687)
                     /locus_tag="PLE23_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82382.1"
                     /translation="MKRYIVVLLYLVLVLGPLLAGVVYSMLYSTGSIGLLSKGFSLVA
                     WQNVLTDDFVKRSFLSTIFVATISLVIGVAAALFVVLKSNWLRRSGIAGIMLHIPLAF
                     PPMVAAFLMFQLLYKGGLLSRIFYQLGLTTDIADFPGLVNDNLNIGVILCMALFVFPF
                     FVVFFVQKMEISGMNRYVTLAASLGAGRYQTDMKIVIPLLLRHAWFNIALYWLILFGN
                     FEIPLILGRQHPAQVSVLIGQKFTKYDLGDLPEAYAISLLYVLVVLVVFLLFLFKNRK
                     IQAI"
     gene            complement(12684..13370)
                     /locus_tag="PLE23_03605"
     CDS             complement(12684..13370)
                     /locus_tag="PLE23_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HMX82383.1"
                     /translation="MAQLSEYVKAHPGKFTIPAEFAGMTLLKSWMTELAGMGSGYFDG
                     TFDSLKYAATSEKLWHYINENRKYYWRNGETFPEKLSAVHQMFANSELDFTLSNNDAE
                     VDNKIAQGVFPGTAKAYVPDVGSIRNSHYLGIPSNSPNKNAALVLINFLISPEAQLQK
                     MKPEVWGDGTVLDVKKLDAHWQQEFSKASVRKSAPSRQTLSQKAIKEPAPEYMIRLYS
                     DFRNKIIGKQ"
     gene            complement(13374..13892)
                     /locus_tag="PLE23_03610"
     CDS             complement(13374..13892)
                     /locus_tag="PLE23_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82384.1"
                     /translation="MKNLIAIFTLFLISCGGNPSSGPVEASGWDEIVKKAQGTTLNMM
                     MWQGDPLINKYMAEWVAPELKKRYNIELRLLPGQGSEIVKILMTGIEQGEKSGSIDVC
                     WINGETFYQLRQINALYGPFVEMLPNAKTIDAEDPFIKYDFQQLTQGFECPWGNVQMC
                     IIYDSVRTPSPP"
     gene            complement(13912..>14113)
                     /locus_tag="PLE23_03615"
     CDS             complement(13912..>14113)
                     /locus_tag="PLE23_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82385.1"
                     /translation="PLGGKKGNLSVRADVFNLFNAENLSGYSNNATQSNQIQAGTAAS
                     GLLVRRNAAPPRQFQFALRYSF"
BASE COUNT         4211 a         2991 c         2797 g         4114 t
ORIGIN      
        1 tgataccgtt cagcaggcga ttttgtaagg atatcaattt cgcgctccag ctgttgaaca
       61 atcaggttct caagtatctt tatccacagt ttttgattct catcttcttt cagaaactgt
      121 tcaatctgct gttttgtaat tactcttacc gtgctgttct ttatagcctg aatgaatagt
      181 tcggatggtt tatctgtcaa aaatgaatcg agcgatgtta tcagattgcc gttgtaggcg
      241 aggcgaatta tttgttcttc accttcgctt acgataaata tgcgcatact cccgttctct
      301 aaatagtaaa gattggtatc tgtacttcca ctgtacttta gatattcgtt cctcttcaga
      361 ataattgtct tggttgacag ttcgatcaat tgtttcatca gtatgttgta gctgttatta
      421 ctgtcgtagt cttgaatgta tttagggcat gctatataaa tagttcggga taagctcttc
      481 cggatataat catttcagaa aattaaacct attattgtaa aaccaaaaga gggacaaaat
      541 tttaattccg tccctcttag gatgtttttt aatgctagta ttttattgta tttttataat
      601 ctttctgctc actgtacttc cgtcggctga taatttcatg atgtaaaatc ctgcaggtac
      661 atcacccaga tcaacaggaa cattcagctg atctgctttg ccggtaatct ttgaatagac
      721 aggttcgcca tagatgttgt atatatcaat agctgtactt acagctttgc caaatacagc
      781 aactacatta aacaatccgt ttgttggatt cggattgatg ttcaggactt tcagatcttt
      841 tatttcgtga gttgctacgg catttatttg aaaatcaatt acttgcgtac aaccagattc
      901 atttgttacg gtgacactgt atttaccgct cttaaggaag gaaacagact tgccggttgc
      961 cccattgctc caagcatagc tatatgtgcc ggcaggtgta ttgagtgcaa tttttccgtc
     1021 cgattttccg gggccggaaa ccggagtgac gttaccggtt acgcctaatg tctgagggca
     1081 attacccaca tcaaaacaat caacatcttt acaagcatta gcatccgtaa tggttacgca
     1141 ataattacct gcagtaaggc cagaaatgcc ggctgtagta gccgaattgg accacaagta
     1201 agtatatggt gcagtacctt ttgacggagt gatagttaca gcaccatcgt tggtgccacc
     1261 aaaagacacg ttcttcagct tagaagaagc tccaagactc aacggacaac cattgatgtt
     1321 gatattatcg acatctaccc agtagtcgcc ggtgatgtat tctgccctgt agcgcagttt
     1381 gatgatttta ccatcataag cagacaaatc tacagctaca ttcttcatat cagctgatgg
     1441 gatatgattt gactgatcga tggtgtacaa tggtttgaat gtaatgccac agtcatcgga
     1501 tatttcgagt accagcttga cagcatcagc acttgtagga ttggtgccgg cgaaatagtc
     1561 aacatatctg taatcgaaag tgagggtgtt acctccgtca agcggcccaa tgttgctggt
     1621 tgtgaaacta aactttgatg aagatgtgaa cagattacct gctatcacat aagttttgtt
     1681 gttgtgacct gcagttattg aatatccttc tccaatccag ccttcaggaa gcagaaattt
     1741 ttcaaaatct tcctttagag gcagtgcagt cggctctggt atggtgagag aaaaggaagt
     1801 agtatcgttg aaggcattgg catcattcac tgcctgcacc caggccttcg ctttgtatgt
     1861 accaggccca taagagctaa acttggtctt gaaggtataa gtgtaagagt ctccgacttg
     1921 aatgacagcg gcaggtacat cttcttcgat taccgcatca ttttcaaact gataatatgc
     1981 ttttacctga tttggcccta caattggctt tttacctgta ttggtatagg taaatttcac
     2041 gaaatcattt tcacttccac attctgatgt agaagtattg gtgagcgcta cggctgtcag
     2101 atctttgtct atctggttgt aaatggtaat attgtcaact gctacgccac tatcgccacc
     2161 aaagttataa aatgtgctga acgctatacg aatacggcaa tttgactcac ctttcagttc
     2221 tgtaagtttg tgacctgcta ttgtccagcc cattgaagta cctgcccatg tcgaaaatcc
     2281 gaaaatattg ctggcagtat tataccactt caacgggtcg ttgtactgac caagcctttc
     2341 ccaggtaagc ccaccatcct ttgagtactc aacccagata ccatgatagt atgtatcaat
     2401 gtcataatta atattaaaag taaggtacgg ctcattttga gcatcagaaa agtcaaaaca
     2461 cggtgattcg agataagcct ctacctgatc tgtaaaacct tccggtattc tggcaaaaaa
     2521 acaattattg ccacttgcag cagtgttgat gttcggcgta ttaggtgtac caaactccca
     2581 gccagtagta cctcttacgt tgttaaatgt ccagcctgat tgtgatggtt cgaaattaac
     2641 agcataaggc aatgatttaa ccaaaggaac atggaataat gtataataag tagtatcgtt
     2701 cagcttattt tcatcgcctt tgagctctgt ccaggaggct atcttaaact cgccttcttc
     2761 cgaatagtca aagccggtat cgaaatcaaa tgtacctgta ctatcggtac tcaatacacc
     2821 ggtatagtaa ccatcaacaa aaggtggaac cgatataggt tgctgattga cactaaacct
     2881 tacatctatt agcgattgtg ggttgccgcc ataatttttt atctgtattt tcagtttttc
     2941 atcattgccg agttcgcagt tgctttttgg tgagaggatt ttagatacac cgacatcatt
     3001 ggtaagcttt gttttgaaat taagcgttag gtcagagctg ccttcacccg agtcacactt
     3061 aaatcttaac aaagccgtat attcagtatt tggtgtaagg cctataattg tagccgtaag
     3121 ttttttgccc gaaacaatag tacctgtacc tttaacaaag ccaaccaggc catattcaag
     3181 ctcataaaca cctgcttggt ctttgtaacc atcataatta aaagtagcag atgtagcttt
     3241 aataccagct acttcaaaca agtaaggcgc atagcataca aggcatggac tcccgtcaga
     3301 aatgcctcct ttgttggcca aaatattgct tacaaaaagc aaacagaaaa ctacaggaat
     3361 aaatattctc ttcatattgg tttaatatga ttaattaata agccaaatat actctcattc
     3421 attcgaataa tttatttttt accttgtatt ttatcaataa aagataaaat tgcattcaaa
     3481 cagaacaaaa aattataacc aactgtttat ttaacccaaa taacgcattg ggaatttaaa
     3541 cgaaaatggt tcgtacaaaa aatccataaa agagtgataa agcgatagtg gattcaggtt
     3601 tttgaacttt tgaaatggat ttgaagtatt aattatttac aattaagagt ataatgcgta
     3661 atctgggttt aactcatcat tacatagtaa ttaatctgat tgatccgtga gcaaatattc
     3721 tatcaaagac ctcgaaagat tgagtggcat caaggcgcat accattcgta tctgggagca
     3781 aagatacagg ctcatcaaac ctcacagaac gcatagcaat ataagatact atgatgaaag
     3841 cgaactgaaa cgcctgcttg acatagcatt gctgaataag aatggttata agatatcgaa
     3901 gattgctaca ttttgcgagc actgtatcag ccatctactg tccggtatag ccaccaatga
     3961 atcagatatc gggcaacatg acgagcttat gattatcatg gtaaccatgg atgaagaaaa
     4021 atttcattcc accattgaca gcaagataaa agaaaacggc tttatccatg tactcgaata
     4081 tatgatcgta ccatttctag aaaaaattaa tttcatccgc cttaccggta ccatcaacac
     4141 tgtacatgaa aattttatca ttaacctcat acgtcagaaa atagtagccg ctacggacaa
     4201 gatcaaaaga aagcaatccg gcaaacccgg ttttttgcta ttcttgcctg aaggggaatc
     4261 acacgaactt atcctgctca ttatgcagtt cctgttgcgt tcaaaagctt acaaaaccta
     4321 ttatcttgga caaaacacat ccttgtatga tgtagaacag gcatataaat tagtaaagcc
     4381 tgattacata tataccattt tatctgaacg gtttacaggc caaccaattg gttcttacat
     4441 tgatgaattg aacagatgct gccctcaatc ggtgctatta ctttccggat accagcttaa
     4501 tgtgctcaat attccgtcag gtaataaatt caaaacgctc aactcatttg ctgaagcaat
     4561 cagttttttg gatgaattat caaaaaattc aatctcaaaa taacaatact ctactgcgaa
     4621 ttgaaacatt tttgctagga ttgcatttaa tgaaattgag atttcaaatt tataattaat
     4681 aattaaatat gaaaaaaatt tatttatttt tgatgttgat tgccttcgct ggtacgcaag
     4741 cttgcaaaag cgacaagaaa gcagatgttg cagcaacgga cattgcaaat gacacattgc
     4801 caccaaatag tgccacaaaa tcgcagattg atgccttgcc ggagatgaaa actgaaaata
     4861 ccgcaggtac cccggccagt actcctgctg acgctatcgt ttttaaaatg gcaaatgcca
     4921 gcgtttcatg gattggtagc aaaggtagcc tgaagactca aacgggcacg ataagcggaa
     4981 aatcgggaca gatagcctac aaagacggtc agatagtgtc aggcaacgta gaaattgata
     5041 tgaacaagat caatgttaca agtctggcgg gtagcgataa aaaagatctg gaagatcacc
     5101 taaaggctga tgatttcttt gatgtcgcca agtatccgac tgcaaagttc gaaatcgtca
     5161 gaatcgagcc tcttgactca agagcatcac gtgaggcaaa tatggccagt ttggtaaaaa
     5221 atacgcacat gatggtaggc aacctaacca taaaggatgt gacacgtagc ataacggtgc
     5281 ctgtcaatat ccacctggca gcagaacgga tcaaaatagc tacttcaatg tttaccatcg
     5341 agcgctcact ttggggcgta aaatatgact tgggtaccgt atccggtaaa ataaaggaag
     5401 caatcgttga tgacaaaata tcgctccagc ttacttttga aggcatgaga gcagcaaaat
     5461 aacttttgtt aaaccaagtt tacaaaagcc ggatctgata taagattcgg cttttgtctt
     5521 tgtaggataa tattataaca cattaagaat ttttccaaaa ataattagta caagaaagcc
     5581 aaaaaaacag cttattaaag catagtgaac cttaatcttt gagcttttaa aatgaatggt
     5641 gagcaatcat taacttgggt aaagagacaa gtgcattatt tgggttaata tcttctattc
     5701 acattccacg aaagtggtaa taaaaaaagc ccggaaattt ccatcatttt ccaggctcag
     5761 ggtagtgtca aacattattt ttatcaggca ggcattaaag ctggtgctgc cttctttgat
     5821 tttgcaaatt cgagcgcgct gaatgtgtaa ttcttttggg caaaatattc caaaaccctc
     5881 ggcaatacgt attgaagttt atcatacgat ttgatgttgt catgaaatac tattattgat
     5941 cccggtacag catgattcac tacatttttc agacagttct ctttggtgat tgtagggtca
     6001 aaatcaccgc tcagtatatc ccacattacg accgaataat tttctgacaa gtgctgtgac
     6061 tgttttcgta acaatttgcc gtacggcggc cgaaacaagt cggagtcaac taactctgca
     6121 cattgatcaa cattttcaac atactcattt acaggtgtat tccagccatt gagatgatta
     6181 aatgtatgat tgcctgtctt atgaccttct tcctttagcc gattaaaaat ttgcgggtat
     6241 ttccttacat tgtcacctac acagaagaag gtggccttgg catcatacgc agctaactga
     6301 tctagaaccc aaggtgtcac ttcaggtatc ggtccatcat cgaaagtaag atatattgct
     6361 cgcttatcat tgggtttgtc ccaataataa gtcggaaaaa tatgtttaac caccttcgga
     6421 gttttgacca gatacataat gctaacagtt acaaaaacag ataattcata caatgaacga
     6481 atgctgccct acatccgctg ctttagattg ctattttatt gaaaaaaaag acaaatttca
     6541 ccggcaggtg acatacacat gataactgaa tcaatacttt aaatacaata catttctgta
     6601 caatactaag atagcgttgt accttatgat tcatttccat tacctttgca ggaaataatc
     6661 agagaaaaaa tgagagtcag attcgctccc agccctacgg gcgcactaca cataggtggt
     6721 gtacgtacag ctttgttcaa ctatctgctt gccagaaaac acggcgggca gtttatacta
     6781 cgcatagaag acaccgacca aaaccgtttt gtacccggag ctgaaaacta tattacagaa
     6841 gcactcgaat ggtgcggact cacattcgac gaaggtccgg agcaaggagg agactatgca
     6901 ccttatcgac agtcggagcg aaaaccaatg tatcgccaat acgctgaaag attggtagcc
     6961 gaagggaatg cctactatgc atttgataca gcagaagaac ttgaagcttt ccgcaccaaa
     7021 gaccctaatt tcatatataa ccattctacc cgtacgcttg cagccaacag cctgacactg
     7081 ccaccggatg aagtaacaaa gcggatagcg gcaggccaac attacgtcat tcggctaaaa
     7141 gtacccgctg aaggcaaggt tgttttcaac gatcttatcc gtggagaggt gagttttgac
     7201 aacagcctca ttgacgacaa agtactgctc aaaagtgacg ggatgcctac ctatcacctt
     7261 gcaaatatag tagatgacca cctgatgaaa ataacccatg tcattcgtgg agaagaatgg
     7321 ttgtccagta caccacatca tgtacttttg tatcgttttc taggctggga agacacaatg
     7381 ccggcatttt cgcatctacc cctgatacta aaacccaacg gaaacggcaa actcagcaag
     7441 cgtgacggag caaaattcaa catgccggtg tttccgttgt cgtggccgca tcatgaagaa
     7501 gaaaattacg aaggagaaag attcaccgga tttcgtgaag ccggattttt gccggaggca
     7561 gtaatcaatt tcctagcctt acttggctgg aacccgggaa ctgaccagga aatattttca
     7621 attgaagaac tcacagaagc tttcagcctt gaaaaaatca ataaatcggg tgcacgattc
     7681 gatttcgaga aagccaaatg gtttaaccac cagtatattc aacaaaaatc tgatgccgag
     7741 cttactgcgt tgatacaacc cgacctgaag gccagaggca taactgctga tactaaattt
     7801 gtagaaaaat tctgtagtct gatgaagagc aggattaatg tcctgacaga atttgccact
     7861 gtcggatatt atttcttcac agaagaactc aactacgacg aagccaatat acagaagaaa
     7921 tggaatgctg aagtagctcc aaaattgcag gaagtaatac gctatattga aaaagcggag
     7981 atggccgacc accagagtat ggaagccaac ttcaagacat tccttgcgga agccggctat
     8041 aaacccggtg aaatactacc attgatgcgt ctggcacttg caggcacgat gcaaggtccg
     8101 ggagtatttg agatggtgga acttcttggc agggaaaatg ttatattaag gatgaacaat
     8161 gctgtacaag cctttaataa gatcataaaa taatatatgt ttttcccaat aggtgacgag
     8221 caggtagaac gcggacataa gccgatattc agttatctgt ttttggctgt caacatattg
     8281 gtattcatct ttgagatatc gctcgatgcc gaatcgctta atgcttttac tttttcgttt
     8341 ggagccattc cgtcggagtt tttgcatggt catcagttgt attctcttgt tacaagtctt
     8401 ttcttgcatg gtgggtgggg gcatttactt ggcaatatgc tgtttttgtg ggtctttgca
     8461 gacaacatcg aagctacgct tggcaatatc cggttcttca ttttttacat agcaggtggt
     8521 attgctgcca ctttattcca cgggttgctc aaccctgcca gcgatattcc tctcataggc
     8581 gccagtggtg ctatatcagc tgtcttagga gcttatctgg tatattttcc acatagtcgt
     8641 atcaaagtac tggtgcttat actgttctcg agctttaggg tacctgcctt ggcctttctc
     8701 ggcatctgga tcgtcaatca gtttattgcc ggatatggat cactcagcca tgtagcagaa
     8761 gaaagtgccg gtgtggctta ctgggcgcac ataggtggtt ttgtatatgg tatcattatc
     8821 ggcttctaca caaggcgtat atacatacct gtactatcgc gcgaagcagc cgagcaggat
     8881 gaaggttatg tgtgatatta gctacttttt attttttccg taccggaatc cagatttcct
     8941 cttctgactc cggggcattg ttcctgtatt ttgctcctaa tacttcaaag tgcggtctgt
     9001 catccaatat gaagtctgat gccggcaacc aactgccgta gatatattga aatactgaac
     9061 gatcggtatt cagcccctta tattgaaata cggcataaag cccttcggga atcaccattt
     9121 tttccattcc gtcaggtaag gttgaaatat ctgatacttc tacagccgcc catttgtcaa
     9181 attcatttgc agggttaaat gcagtaaaat aacccgatgg ataaacagaa acggataaga
     9241 gatcatcact cagtttgttc tgtatgttct tcctttcggg cataaatgat tgccagagtt
     9301 cacctacacg gtattcactg atgctcatca ccaaacgctt accgatgagt cttttttcat
     9361 gtataatcct gattgtaggt atcatgtatt tgtttaaaaa tttttagaaa tttcaactcc
     9421 ggataatgca aatctattgc tatttcttgt ccaatttttc gatatcaaaa tgttctttga
     9481 aaaattcatc acaccaaaga caaatattgt cgagttcttc tgtccttgcc atggcataag
     9541 cccttgatat accgaccgac tcgcccatac cgagtggatc tccccgatca atacgctgaa
     9601 acacaggcga agctgaaacc ctggcaagca cttggcttat cttttcggtt cgggcatcgc
     9661 ccattgcctt gtaagtcccg ggacaacagg gattgatctt ccacaattga atgctgattt
     9721 cttgcggaat atcttcgcca ccattcagga agccaatgcc accagataac acggcacaga
     9781 aattatgttt cggatctttt ttccagatgc tgttcttcaa tgcgcgtccg cgtgcccagt
     9841 tgccacccag ccacatgtct tcggtagcac cccaatagcc ccagctcagt ttcggcttca
     9901 cgtaattttc cttatcgata agcggatctt tgtcactgcc atctacaccc cgactgtcga
     9961 ataactcggc aaggttcatc aggtgttcgc ctgccttctt gtgataacgg tctatacttg
    10021 ctatgtcaaa cctcgtaatg cctttttcta tcaatacatc cagaatctct ccggtcaata
    10081 gatcgccatt ggtctgcaac attatctgcg aacgactgcc atacctttcc ttcagagcat
    10141 caagaatcag gaagagttgc tttttcttgg ctatgggttc gccgcctgag agtatgatcc
    10201 ggtctatttc atccggtagg ttggaaatga tttgcaggca ttcttcgtca ctcaacctgc
    10261 caccattcgg acctgaatcg ttatagcagt gtacacactg atcattgcag acttgcgtaa
    10321 agacccagta caatgattca agatgattaa aatttgtttt tttcacgttt tgtcagcatt
    10381 aatattgtcc cagaaagtct tttcttgcat aacacccgtt tcaggactgt tgataaactc
    10441 agatttatct ttgtaaaatg ttagcctgcc actgtccatt accgcaaaat gattgcccat
    10501 aatcaatgct tctttaagat cgtgtgttac aaatactgtt gtgatattaa atttttcggc
    10561 catttcttta taaaacatct gcatggtggt tcttgtttcg aaatcgagac ttgaaaaggg
    10621 ttcgtcgagt aacatacaat ccggattaac aatcagtgac ctaccgaatg ccacacgttg
    10681 tttttgccca ccactcaaca gctgcggatt ttgattttca tatcctgtca ggccgagttc
    10741 tgatatcagt ttgtttgctt tctgtaatat ttcttcctca cccaacttcc ttgcacggag
    10801 tccgaaagct atgttttcga agacattcag gtgtggaaag agtaaggcat cttgatagag
    10861 ataaatgata tttcttttgt tcaagggcaa atgagtaatc agtttatgct ggtgaaatac
    10921 atctccttca caggcaggaa taatgcccgc aatgattttc agcaacgttg tttttccaca
    10981 accagatttt cctaagatgg caagcgtctt tccctgttca agcgaaaagc taatatcttt
    11041 cagcacttca tttttgtcga agcgtttgct tatatttttt acctcaagcc acatttgatg
    11101 ataatttatg ctgaacaatt gacaacatta ccggcggaat cagcaataca agagaagcag
    11161 tagctgcata cgcaatgttt gcctccgcaa tatactggta tatcctcagg atggcagttt
    11221 gagcaatgcc ccggcctatg accagactga taccgtaatc gaaccaactg atgagaaaag
    11281 tctgtatgag tgaaagctta atcaggcgca tagtgagtgg tagtgtgaca cgtacaactg
    11341 tttggtattc ggacgctccc aatgacatgg atagtatctc ctgatttttc agttcatgat
    11401 tccaatgttt tgaaaaaagt agcactacaa atggaaagct aatgagcaat tgggcaatta
    11461 aaacaccggc ataagtcccc ttcaatccgc tgcgaataaa caaaaactga atgcaaaagg
    11521 cataaataac aggcgaaaaa gaataaggca aatatgcaat cgaatgcaaa atatctctat
    11581 aacgatgcct gctcaattgc ctgctgatga agaatgccat tgcggtagcc aatattgcta
    11641 tacttgaaga caatacaact gatttgagca acacccacca aaaatcattt cctaaaaata
    11701 atccgtgcca tgcattcaaa gaccaatgac ccggcaacac atcagggaaa aaccaggtac
    11761 cggctattga cagaaacaac aggaacacaa aaggaatcat gaccaggttg attaacacta
    11821 ttattcgaat cctagctttt gttttcatat tgcttgtatt ttcctgtttt tgaataaaaa
    11881 caacaaaaaa accactaaca ccactaacac gtacaataac gatattgcat acgcttcagg
    11941 caaatcaccc aaatcgtatt ttgtaaactt ctgacctatg agtaccgata cctgagccgg
    12001 atgctgtctg ccaagaataa ggggaatttc aaaattgccg aataatatca accagtataa
    12061 ggcaatatta aaccacgcat gtctgagaag caaaggaatg acaatcttca tatctgtctg
    12121 ataacgaccg gcgccaagtg aagccgcaag tgttacatat ctgttcatgc cggatatttc
    12181 cattttctga acaaaaaaca ctacaaaaaa aggaaatacg aacaatgcca tacaaagtat
    12241 cacaccaata ttaagattat cattcaccag ccccggaaaa tccgcgatat ctgttgtaag
    12301 accaagctgg tagaatattc ttgacagcaa accacctttg tacaatagtt ggaacatcag
    12361 aaatgcagca accataggtg ggaaagcaag cggtatgtga agcattattc cggcaatacc
    12421 tgaacgcctc aaccaattgc ttttcaacac aacaaacaaa gctgccgcca caccgatgac
    12481 aagacttatg gtagctacaa aaatggtaga aaggaatgaa cgttttacaa aatcatctgt
    12541 taaaacattt tgccaagcta ccaaagaaaa tcctttggaa agcaaaccga tgctaccggt
    12601 actatacagc atactgtaaa ccacacctgc caacagaggc cccaacacga gtaccagata
    12661 aagcaacacc acgatatacc ttttcattgt tttccgataa ttttatttct gaagtcggaa
    12721 tagagtctta tcatgtattc aggtgcgggt tctttgatgg ctttttggct gagtgtttgc
    12781 cttgatggag cactttttct aacagaagcc tttgaaaatt cttgttgcca atgcgcatcc
    12841 agcttcttca catcgagtac ggtgccatca ccccatactt ctggtttcat cttttgcaat
    12901 tgcgcctccg gcgaaatcag aaaattgatc agtaccagag cagcgttttt gttcggcgaa
    12961 ttagaaggaa taccaagata atgcgaattg cggatgctgc caacatcagg aacataagct
    13021 ttggcagtac ccggaaaaac gccctgtgct atcttattgt ccacttcagc atcattgttg
    13081 ctcagggtga aatcgagttc gctgttggca aacatctggt gtactgccga taatttttcg
    13141 ggaaacgtct caccatttcg ccagtaatat tttctgtttt cgttgatgta atgccagagt
    13201 ttttcgcttg ttgcagcata ttttaatgaa tcaaacgtac cgtcgaaata accggaaccc
    13261 atacctgcga gctcagtcat ccaggatttg agcagggtca ttccggcaaa ctctgcagga
    13321 atggtaaact taccaggatg agcttttaca tactcgctga gttgagccag gtttcatggt
    13381 ggtgaaggtg tccggaccga atcatagatg atgcacatct gcacattgcc ccacgggcat
    13441 tcaaaacctt gtgttagctg ctggaagtcg tattttatga acgggtcttc tgcatctatt
    13501 gtcttggcat tgggaagcat ttccacaaac ggaccataga gggcattaat ctgcctaagc
    13561 tggtagaatg tctcaccatt gatccaacag acatcgatac tgccagattt ttctccttgc
    13621 tcgattccgg tcatgagtat tttcactatt tcacttccct ggccgggcaa gagccttagt
    13681 tcaatattgt agcgtttctt caattcagga gctacccatt cggccatata tttattgata
    13741 agtgggtcac cttgccacat catcatgttc agggtagtgc cctgggcttt cttcacgatt
    13801 tcatcccaac cggaagcctc taccggcccg gaggaaggat ttccgccaca ggatattaaa
    13861 aacaaggtaa aaatggctat gagatttttc atctgttttg gtgaaatctg attaaaacga
    13921 atatctgagc gcaaactgaa attgtcgtgg aggagcagca ttacgacgaa caaggagtcc
    13981 ggaagcagct gtgcctgcct gtatctgatt gctctgtgtg gcattatttg agtagccgct
    14041 gagattttcg gcgttgaaca gattgaatac atccgctctt accgacaagt ttcccttttt
    14101 accgccaagc gga
//
LOCUS       DAOQQY010000039        13785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1489_length_13785_cov_7.135397, whole genome shotgun sequence.
ACCESSION   DAOQQY010000039 DAOQQY010000000
VERSION     DAOQQY010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13785
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_13785_cov_7.135397"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..76)
                     /locus_tag="PLE23_03620"
     tRNA            complement(2..76)
                     /locus_tag="PLE23_03620"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Ile,seq:cat)
     gene            179..814
                     /locus_tag="PLE23_03625"
     CDS             179..814
                     /locus_tag="PLE23_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HMX82386.1"
                     /translation="MIRNILFDLGNVLIDIHLPATEDALRNIQGMNMQALDTLINQHK
                     WFDRYECGEMSEESFVNAIQRCCIPVPQRNIIVNALNALLKGIPASRFAELEKLRRTY
                     NLYVLSNTNETHLNWIHRYLIREYNIAQFESTYFDKVYYSHLVGMRKPDMAIFTHIMS
                     NTGIDPAETLFVDDNFDNITAGKLAGLHVHHHNPEFEIFDILPDILKVANT"
     gene            811..1281
                     /locus_tag="PLE23_03630"
     CDS             811..1281
                     /locus_tag="PLE23_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HMX82387.1"
                     /translation="MKNFKQKLTPMMAMEKMRKYCDYQERCHQEVRTKLLTLGVYGQD
                     LEEIIAELIQEGCLNEERYARAYTRGKFRIKKWGRIKITHQLKAKHVSDYCIQAGLEE
                     IDEEEYYEAIRYNINIAISMGHKSDFEISQYLMAKGFESGLINEALADIHRDAK"
     gene            complement(1322..2923)
                     /gene="nadB"
                     /locus_tag="PLE23_03635"
     CDS             complement(1322..2923)
                     /gene="nadB"
                     /locus_tag="PLE23_03635"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HMX82388.1"
                     /translation="MINTDILVIGGGIAGMTFAIKIAKERPDLEITILTKTEDDESNT
                     RYAQGGVAAVWNKEKDSFDKHLEDTLIAGDGLVDEKVAAIVVNEGPQRVQEIIDWGTR
                     FDQTKEHAYDLGREGGHSENRILHYKDLTGWEIQRALIEESRTLKNITVHEHYFALDI
                     ITQHHLGHNITRVTPGIECYGAYVFDKNLSKFDTILAKITVIATGGCGQVYKSTTNPK
                     IATGDGIAMVYRAKGRVENMEFVQFHPTSLYNPAGENPSFLVSEAVRGFGAVLKHRDG
                     TEFMQKYDPRLSLAPRDIVARAIDNEMKLNGEDCMYLDCRHLDKEKFIEHFPTIYNKC
                     LSIGIDAMQQMIPVVPACHYMCGGIKVDEAGRSSITHLYACGECTSTGLHGANRLASN
                     SLLEAMVFAHRIFLDVKDNIDALSFVEGIPDWDTEGTTDPKEMVLITQSLKELKEIMS
                     SYVGIVRSDVRLKRALDRLYILYQETEDLYGKTSISPQLCELRNLITIGYLITRSAYS
                     RKESRGLHYTTDYPDKKPFIESTIL"
     gene            2994..4115
                     /locus_tag="PLE23_03640"
     CDS             2994..4115
                     /locus_tag="PLE23_03640"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMX82389.1"
                     /translation="MRIAVNTRLLLKGKLEGIGWYTYEVLKHMVETHPDDEFIFLFDR
                     PFDEKFLFGKNVTGIVVPPPARHPVLWYVWFEWSLPPILKKLKADVFLSPDGYCSLKS
                     KVPVLMTMHDLAYLHFPNQVAWLTQKYYERYVPKFLKRAEKVAVVSDYVKNDIVSNLG
                     IPAQKIFRAYNGVRQEFQPINNSEKEKTQNQYTSGKEYFLYYGAIHPRKNILNLIAAF
                     EQYKSNNGGHKTLLLAGRMAWKTSEIENKIKQSPFSSDIYHVDYLGDELPRVVASAFA
                     VVYVSMFEGFGLPVAEAMKCGVPVITSNVTSMPEVAGDAAICVNPHDVTEIANALCRL
                     DQDNDLVRELSGKGIVQANKFDWKICSDQIYKELRQLVE"
     gene            complement(4135..4938)
                     /locus_tag="PLE23_03645"
     CDS             complement(4135..4938)
                     /locus_tag="PLE23_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMX82390.1"
                     /translation="MVILAYALAALVGISLGLIGSGGSILTVPILVYVMGIEPVHATA
                     YSLFIVGTTALVGAIQKSFQSTVDYKTIILFGIPSLLAVYTTRLWIVPAIPDVLFNIS
                     GFVFTKSLFLLILFAVVMILASYSMIKPAKIDNAQTAETRKINYKMLFLQGIAVGGLT
                     GLVGVGGGFLIIPALVFFARIPMKTAIGTSLFIIAANALIGFTGDLQGDQNMDWPLLF
                     GFAAVSIIGIFIGNYLCKKIDGSKLKSVFGWFVLAMGIYILLKELIFKF"
     gene            5102..5172
                     /locus_tag="PLE23_03650"
     tRNA            5102..5172
                     /locus_tag="PLE23_03650"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5134..5136,aa:Cys,seq:gca)
     gene            complement(5234..6322)
                     /locus_tag="PLE23_03655"
     CDS             complement(5234..6322)
                     /locus_tag="PLE23_03655"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HMX82391.1"
                     /translation="MKRNILLCMLSVMLCCSSTISAQQIVRPVVVEEATGTWCGFCPR
                     GAVSMDYMSDKYGDDFVGIAVHNGENDPMVVDAYDAGLTKFPNFSSFPSAVVARTHLL
                     DPKDIEQPFLDEAAIPATVELNAKATYDKVTRQLFVTATAVPSKNYVAPKFFIALIED
                     GVSGTSSGYAQANYYAGGNYGQMGGYEKLPNPVPADKMVYNHVARALIGGFNGIGQSI
                     KSPWLANKPATYEYDKYSIPEAFNISKCHVVVGVLSNENKFLNVVKLPLEEALASKEL
                     NADQKVVISPNPVKDIAYIQVNNSHANELSIRVFNNLGLQVAAQNYGRLSGEISLPLL
                     TADLAAGVYFVHINLGEERVVKQIAVVK"
     gene            complement(6474..7844)
                     /locus_tag="PLE23_03660"
     CDS             complement(6474..7844)
                     /locus_tag="PLE23_03660"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMX82392.1"
                     /translation="MRGIRNILFFFGVVLVTGALFWAFTCTRPGRLTDAESQAKEVIL
                     AWNKLYLELDRVTDGYYAPVSARNFAYISLTAAVASKAYYKDTFIENDSFHGLQIPQY
                     SEILDYNLVDLLNSSYAESFRRFFQYAPQNSFAKINELEDKLSQMQNSTEELLVKRIN
                     SRKYGLMVANAVYLWSATDSYGHEVHKHLYDNNLTLIGRRNSWLIRTEPDTSQLLPYW
                     GKVRPFLINVNTYKLGPPPAYSESPSSEMYKEALALYTLSKPLSEENRWIAEFWNDDI
                     RGLTFSPVARWLSIAGQVVEEEQISTETILKLYLELGISLCDVSIIVWKAKYEFAQKS
                     PQTYINKLIDKAWRPIYENPNFPTYPSGHAAFGAASSLVLSKYFGEKYSMTDDSHIDR
                     KEFNGKPRSYNSFREMALENAYSRTAAGFQSRSSAEEGLKIGSVVAARILDIPIFKDK
                     TSKKNH"
     gene            complement(7908..9287)
                     /locus_tag="PLE23_03665"
     CDS             complement(7908..9287)
                     /locus_tag="PLE23_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMX82393.1"
                     /translation="MQIRTRLTLQFILIVVIILQLSLFFIYFKFKDHVEDQFYNDLKS
                     KAYMTAEMLVNIQNVEPTEQTASDKTEYYLESKENFLIFNHENKKIYEFNKLKENEIP
                     VQTLEQVKNSGEYRFTHGKFNSIGIFYTNKFDKEYTVISEAVFNSTDLANLRNIIIID
                     FCLIIIILTVSGWLFAGNALRPVTNIMNQLDKILPSDLSQRLQTTGNKDELWRLGDTF
                     NKLLDRIETAFKHQKSFLSNISHELKNPLTVITSQLEVVLAKERSNEDYRKTLSSVLE
                     DVRDLSVVSDQLMQLARIKNNINQVEFETIRLDEVIWEARSAIIRSFPHYKINVVYNQ
                     LPDETEHLEIQGNEQLLKTALQNIMDNGCKFSKDNQVKVELSGSGNVWVKISDHGPGI
                     SDAEKKLVLSAFYRSPENSMIKGSGVGLALVDSILNIHHARLIISDNEPKGTVFTILF
                     DEGLIAANG"
     gene            complement(9291..9974)
                     /locus_tag="PLE23_03670"
     CDS             complement(9291..9974)
                     /locus_tag="PLE23_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX82394.1"
                     /translation="MKILIIEDHPKTVQSIKQGLEENHIVVDFAYDGYTGKLLAERNK
                     YDAIISDVILPHTNGIDLCRHFRNIGIKTPIIMVSALDTTEDKIVGLDAGADDYLAKP
                     YEFRELLARIKALTRRRIDELQAGHILRYHDVEIDLKTKKVKRKEKIIKLTPKEFALL
                     EYFIRNKEKVITKQEITECVWDINFDTGTNLVEVYVNYLRNKIDKGFDEKLIHTSFGM
                     GYIFHQEQD"
     gene            10336..10938
                     /locus_tag="PLE23_03675"
     CDS             10336..10938
                     /locus_tag="PLE23_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82395.1"
                     /translation="MKTLHYFQIATLAFLLVFSACSKDNGTNDPGTPSTGDPVTLQGV
                     NYEASGTVKYVDTNTGKTETMTYTKVTAQYKDQSHIWPGTFYTFLTFTNLTPNQTISF
                     VFPGKELPKSGIHKIGPWPIATNGITEADKLKDGEMAIFFNGRLAGSKRADFKSINVL
                     NDNGKILLDFGNEVDVFDNITGDKAGNIILINVTKTTKKL"
     gene            11170..13050
                     /locus_tag="PLE23_03680"
     CDS             11170..13050
                     /locus_tag="PLE23_03680"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82396.1"
                     /translation="MKFNCLFWVILTACVSCNDHSIHVKKSNSASEITINQAYQQMVL
                     RNDLPADSMEYYFRSLENMTTDIPQQLAIKNLCRGAYFRKKASFELAISYLNSTLGYL
                     SSNDSLTSEALLGLGICYKHLGDFPKALSYFQKSVQISENNKDKHGLASTFAAMAQMY
                     FEKNDIKRSREFISKVFELYNNHQTGVAYFIALHTLANIEGQSNNFAKALEIDKQGIE
                     LAIKYGNDAVKVSFQDNMARCYLYGYQDYEKALYYFNKNLKIDKKLNNPNWIADTYIN
                     LAEVETARKDFRSAEKSLKQAIDISLQSKQFINISKAYKALSELYKTQGRYDKALSAN
                     EAYTKHYQNVINEKSEQAFAQYNLLFETEKKEKEIAETKLKSKQKNIWLILLTGGLLI
                     GMVIYRAQRNKSKHKQHQLLLENRLLQEEAQIQMQQQRLEISRDLHDSLGSQLTLMNT
                     VLDSIKADSRLDEKTNSKIRKLSDFSEDSISELKSVLWVLNTNEIRMKDLKYKLLNLI
                     KNAGEAKEDMQFLLNMNVNENKPLSGKQAINIFRVVQEIVNNAIKYSEAEVIKINLFE
                     ENNALCLNISDNGNGFDLETEKSKSFGLNNIQYRIEEIGGFVKISSSGGTTFNIQIPL
                     KS"
     gene            13047..13694
                     /locus_tag="PLE23_03685"
     CDS             13047..13694
                     /locus_tag="PLE23_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX82397.1"
                     /translation="MIRIGLVEDKPFSLKALEEKLSRYNDLHIVFTAKNGFDAIAKLA
                     DHKETDLILMDIEMPDMNGIEATGIIKQQYKNIKIVMITIYDDDNYIFDAIQAGADSY
                     ILKETKAEKIYETIQDTLNGGAVMSPSIAVKALQLLKINGNVRSDDTTPEIILSDRET
                     EILEQLAKGLTNKRIAEILFISPFTVKRHIENIYQKLQAHNRIELLDKARKNNLL"
BASE COUNT         4335 a         2828 c         2596 g         4026 t
ORIGIN      
        1 gtgggcccac caggacttga acctgggacc ccctgattat gagtcaggtg ctactaacca
       61 actgagctat aggccccact ttttacaaat tcaatcaatg tttttgtaaa aacgattgca
      121 aatttacatc tttgtttgag ttttttcaac ttattctaaa aagatttatt catattttat
      181 gattcgtaat attctgttcg atcttggaaa cgttttaatt gacattcatc tgcctgctac
      241 agaagatgca ttgaggaata ttcagggtat gaatatgcaa gcacttgaca cgctgatcaa
      301 tcaacacaaa tggtttgatc ggtatgaatg tggtgaaatg tcagaagagt cttttgtaaa
      361 tgccatacaa agatgctgca tacccgtacc ccaaaggaat attattgtca acgccctcaa
      421 tgcactgcta aaaggcattc ctgcctcaag atttgctgaa cttgaaaaac tgaggcgtac
      481 atataatctg tatgtactca gcaatacaaa cgaaacacac cttaattgga tacaccggta
      541 tctgattcgt gaatacaata ttgcacagtt cgaatctaca tatttcgaca aagtatatta
      601 ctctcatctg gtgggtatgc gaaagcctga tatggcaata tttacccata ttatgtccaa
      661 tacaggtatc gatccggcag aaacgctgtt cgttgatgac aatttcgaca acattacggc
      721 aggaaaattg gctgggttac atgtacatca tcacaatccg gaatttgaaa tatttgacat
      781 tttacccgac atactgaaag tggccaatac atgaaaaatt tcaaacagaa gcttacaccg
      841 atgatggcaa tggaaaaaat gcgcaaatac tgcgactacc aggaacgatg ccatcaagag
      901 gtaaggacca aactcctcac gctcggagta tatggccagg atcttgaaga aataatcgca
      961 gagttgatac aggaaggttg ccttaatgaa gagagatatg ctcgagctta caccagagga
     1021 aaattcagga taaaaaaatg gggccgaata aaaataacgc accaactgaa ggccaaacac
     1081 gtttctgact attgcatcca ggccgggctg gaggaaatag atgaagaaga atactacgaa
     1141 gccatccgct acaacattaa tatagcaata tcaatgggtc acaagtctga ctttgagata
     1201 agccaatact tgatggccaa aggcttcgaa tcaggcttga taaacgaagc acttgcagat
     1261 atacaccgtg atgccaaata agcccttagg ttttagcttt taaaggctgt tggcccctcc
     1321 gttaaaggat cgttgattca atgaagggct ttttgtccgg ataatcggta gtataatgca
     1381 gaccacggct ttcttttctc gaatatgctg acctggtaat cagataaccg atggtgatga
     1441 gattgcgcag ttcgcacaat tgtggcgaaa tacttgtttt tccgtaaaga tcctccgttt
     1501 cttgataaag tatgtaaagc ctgtcaagcg ccctcttgag acgaacatca gaacggacaa
     1561 tacctacata gctgctcata atttccttca gttctttaag actttgggta atgagtacca
     1621 tctcctttgg gtctgtagta ccttcagtat cccagtctgg aatgccttcg acaaagctga
     1681 gagcgtcaat gttatccttt acatcgagaa atattctatg agcaaatacc atggcttcga
     1741 ggagtgaatt ggatgcaaga cggttggcgc catgcagccc ggttgatgta cattcgccac
     1801 aggcatacag atgtgtgatg gatgaacgac cggcttcatc tactttgata ccgccgcaca
     1861 tatagtggca ggcaggcacc acaggtatca tctgttgcat cgcatcgatg cctatactca
     1921 ggcatttatt ataaatagta gggaaatgtt caataaattt ttccttgtcg agatgcctgc
     1981 agtcaagata catacagtct tcaccattga gcttcatttc gttgtctatg gcccttgcta
     2041 caatatctct tggagccaga gacagtcttg ggtcatattt ttgcataaat tccgtaccgt
     2101 ccctgtgctt caatactgca ccaaaacctc gtaccgcttc cgagaccaag aaggatggat
     2161 tctcacctgc aggattgtaa agcgaggtag gatgaaactg tacaaactcc atattttcca
     2221 cacgcccttt agcacgatac accattgcta tgccgtcgcc cgtagcaatt ttcggattgg
     2281 ttgttgattt atagacctgt ccgcagccac cggtagctat tactgttatt ttggcaagaa
     2341 ttgtatcgaa ttttgaaaga ttcttgtcaa aaacataagc accatagcat tcaatacccg
     2401 gtgtcacacg tgttatgttg tgaccaagat gatgctgtgt gataatatcc agagcgaaat
     2461 aatgctcatg tacagtgatg ttctttagcg ttctactctc ttctatcaaa gcccgttgta
     2521 tctcccatcc tgtaaggtct ttataatgca atatgcgatt ttctgaatgg ccaccttctc
     2581 ggccaaggtc ataggcatgc tcctttgtct gatcaaatct cgtcccccag tcgattattt
     2641 cctgaactct ctgaggccct tcattgacta ctatggctgc taccttttca tctaccaggc
     2701 catcacctgc tatgagtgtg tcttcaagat gcttgtcaaa cgagtctttt tccttattcc
     2761 atactgctgc tactccaccc tgagcatatc gggtattgct ttcgtcatct tcagtctttg
     2821 taaggatagt aatctcgagg tcaggtcgtt cttttgctat tttaatggca aaggtcattc
     2881 ctgctattcc gccaccaatg accaaaatat ctgtattaat cacgtcttaa ttgttagtat
     2941 tgaggtattt ttggtaaaat taattgattt ctgtagaaag tatgtaaaaa agtatgagaa
     3001 tagcagtaaa tacaaggtta ttgttgaaag gaaagttgga gggaatcggt tggtacactt
     3061 acgaagtact taaacacatg gtagaaacgc accctgatga tgaatttatt ttcctgtttg
     3121 accgtccatt tgatgaaaaa tttttatttg gaaaaaatgt taccggaata gtggttcctc
     3181 ctcctgcaag gcatcctgtt ctttggtatg tatggtttga atggtcacta cctcctatac
     3241 taaaaaagtt aaaagccgac gtctttttga gccctgatgg ttattgtagc ctgaagagca
     3301 aggtaccagt cctaatgacg atgcatgatc tggcctatct tcattttccc aatcaggtag
     3361 cctggcttac acaaaaatat tatgaacgat atgttccaaa atttttgaaa cgcgccgaga
     3421 aagtagctgt tgtgtcagat tatgtaaaaa atgatatagt tagtaatctg ggtattccgg
     3481 ctcaaaagat attcagagcc tacaatggtg tcaggcagga atttcagcca ataaataatt
     3541 cggaaaaaga aaaaacacaa aaccaatata cctccggtaa agaatatttc ctttactatg
     3601 gcgctataca tccccgaaag aatatcctga acctgatagc agccttcgag caatacaaat
     3661 ctaacaatgg aggccataaa acactactac ttgccggccg tatggcatgg aaaacttcag
     3721 aaattgagaa taaaatcaaa caatcgccat tttcatctga catttaccat gttgactacc
     3781 ttggcgatga acttcctcgt gtagttgctt ccgcattcgc tgtagtctat gtttcaatgt
     3841 ttgaaggctt cgggctgccc gtagcagagg ctatgaaatg tggggtaccg gtaataacct
     3901 ctaatgttac atcaatgccg gaggtagcag gtgatgctgc aatctgtgtc aatccgcatg
     3961 atgtgacaga gatagcaaat gccttatgca gactcgatca agacaatgac cttgtgcggg
     4021 aattgtccgg aaaaggaata gttcaggcca acaaatttga ctggaaaata tgctctgacc
     4081 agatatataa ggaattaaga cagctcgttg aatagtattt tgggtggttt caaatcagaa
     4141 tttaaatatt aattccttca atagaatata gatacccata gcgagtacaa accatccaaa
     4201 taccgatttc agctttgatc cgtcaatttt tttacaaaga taattaccaa tgaatatacc
     4261 gataatagaa acagcagcaa aaccgaacaa caatggccag tccatgttct ggtcaccttg
     4321 caggtcccct gtaaagccta tcaacgcatt ggctgcaata ataaatagcg aggtgccaat
     4381 agccgttttc ataggtattc tggcaaaaaa taccagggca gggataatga gaaatccgcc
     4441 ccctactcct accaaacctg tcaggccacc tactgctata ccttgaagaa ataacatttt
     4501 ataattaatt ttccgcgttt cagcagtttg ggcattatca atcttagccg gctttatcat
     4561 tgagtaggaa gccaatatca tgaccacagc aaacaaaatc aacaaaaaca aagactttgt
     4621 aaacacaaaa ccggaaatgt tgaaaagcac atcaggaatg gccggaacga tccacaatct
     4681 tgtcgtataa accgccagca aagaaggaat accaaataaa atgatggttt tgtaatcaac
     4741 tgtactctga aaactcttct gtatggcacc taccaaagcc gtagtgccta caataaaaag
     4801 cgaatatgcg gtagcatgca ccggttctat tcccataaca taaactaaaa tcggtacggt
     4861 aagtattgaa cccccactac ctatcaaccc taatgaaata cctaccaatg cagccaaagc
     4921 ataagccaat attaccatac tcgaatattt ttacacaaaa gtccattaaa aaatcggcaa
     4981 acacggtaat aaatatcaca aacccaacct ctgatttaaa tattttttta atgtatgagc
     5041 aactttttat taaaagtggc gtaaaataaa aaaagcgttt atatttgcgc ttcgttttaa
     5101 tggtcgtgtg gccgagtggc taggcagagc tctgcaaaag cttctacagc ggttcgaatc
     5161 cgttcacgac ctctacaaaa aaagatgcac cggagacgga tgcatctttt ctttttttgg
     5221 tctgattgtg tttttatttc actacagcta tttgttttac cactcgctct tcgcccaaat
     5281 taatatgtac aaaatacaca cccgctgcaa ggtcggctgt aagtaaagga agtgagattt
     5341 ctccacttag cctgccataa ttttgtgcag ctacctgtaa tccaagatta ttgaaaaccc
     5401 gaatgcttaa ttcgtttgca tggctgttat tcacctgaat gtaggcaata tcctttaccg
     5461 gattaggtga aatgacaact ttttgatctg cattgagttc tttgcttgca agtgcttctt
     5521 caagtggtag tttcactaca ttcagaaatt tattttcgtt gctgagtacg cctaccacta
     5581 catgacattt agaaatattg aacgcctcag gaatggaata tttatcatat tcataggtag
     5641 ctggtttatt ggccagccaa ggtgatttga tggattggcc gatgccatta aaaccaccta
     5701 tgagcgctct tgctacgtga ttgtacacca tcttgtcagc gggtacagga ttcggtagtt
     5761 tttcatatcc acccatttga ccataattgc cgccggcgta ataattggcc tgtgcgtatc
     5821 ccgaactggt acctgacaca ccatcttcaa taagtgcgat gaaaaatttc ggtgccacgt
     5881 agttttttga aggtactgca gtagcagtaa caaagagttg tctagtaact ttatcataag
     5941 tagccttggc atttagctcc acagtagctg gtatggctgc ttcatcaagg aatggttgtt
     6001 cgatgtcttt cgggtctagc agatgagtac gtgcaaccac cgcggatgga aaactactga
     6061 aatttggaaa tttggtaagc ccggcatcat aggcatctac taccatcggg tcattttctc
     6121 cattgtgtac ggctataccc acaaagtcat caccatattt gtcagacata tagtccatac
     6181 tgactgctcc gcgagggcaa aaaccacacc atgtgcctgt agcttcttct acaactactg
     6241 gccttacaat ctgctgtgct gatatagttg acgagcaaca aagcattaca gacagcatac
     6301 ataaaagtat atttctcttc ataattgata attttcatca aaactaaaaa ttttaataat
     6361 aataagcaat tatatcaggt ttttataaga aattttatct aaaacaccct gaagattcag
     6421 taaatttcta tagtacaaca ctggtattga gaaaaagaaa gcagtagtaa ggcctaatga
     6481 ttttttttac ttgttttgtc tttgaaaatc ggaatgtcca gaattcgggc agctactaca
     6541 gatccgattt ttaatccttc ttcagcacta ctcctgcttt ggaatccggc agcagtacga
     6601 gagtaagcgt tttcaagtgc catttctctg aaggaattat aacttcttgg cttaccgtta
     6661 aattctttcc ggtcaatatg actatcatct gtcatactgt atttctcgcc aaagtatttt
     6721 gacaacacaa gcgaggaagc tgctccaaat gctgcatgcc ctgatgggta agttggaaaa
     6781 tttggattct cgtaaatcgg tctccatgcc ttgtcaatga gtttgttgat atacgtctgc
     6841 ggactctttt gtgcaaattc gtatttcgct ttccacacaa tgatcgatac atcacagagt
     6901 gaaattccga gttcgagata cagtttaagg attgtttcag ttgatatttg ctcctcctcc
     6961 actacttgtc ctgctataga taaccatctt gctaccggag aaaatgtaag gcctctgatg
     7021 tcgtcattcc aaaattcagc tatccagcgg ttttcttcac ttaatggttt tgacaatgtg
     7081 taaagtgcta aagcttcctt atacatttcg gaagaagggc tctcagaata agccggtggc
     7141 ggaccaagct tatatgtatt gacatttatc aaaaatggtc gaaccttgcc ccaataaggc
     7201 aacagttggc tggtatcggg ttcagtacgt attagccagc tgttacgtct gcctattagt
     7261 gtaaggttgt tgtcatacag gtgcttatgc acttcgtgac cgtacgaatc tgtagctgac
     7321 catagatata ctgcattggc taccatcagc ccgtatttcc tcgaattaat tctctttacc
     7381 agtagctctt ccgtgctatt ttgcatttga ctgagtttgt cttccagttc atttattttt
     7441 gcaaaactat tctgcggagc atattgaaag aatcgtctaa aactttcagc ataactactg
     7501 tttaacaaat cgacaaggtt atagtccagg atttcactgt attggggtat ttgtaatcca
     7561 tgaaatgaat cattttcgat gaatgtatct ttgtagtagg cttttgatgc tactgctgct
     7621 gtcaggctga tatacgcaaa attccttgct gatacaggtg cgtagtatcc atctgttacg
     7681 cggtcaagct caaggtaaag cttgttccag gcaagtatta cttcctttgc ctgtgactcg
     7741 gcatctgtca accggccagg tctggtacat gtaaaagccc agaaaagtgc acctgtgacc
     7801 agaactacac caaagaaaaa taatatattt cggatacctc gcacagctta aatacgatgt
     7861 tattttttag tttttaacat ttccctacct atcattataa aattatttca accattggca
     7921 gcaattaatc cttcatcgaa taatatggtg aacactgtcc ccttcggctc attatctgag
     7981 ataatcagcc tggcatgatg aatattaaga atactgtcaa ccaaagcaag acctactcca
     8041 gagcctttga tcatactgtt ctctgggctt ctgtaaaaag ctgagagtac gagttttttt
     8101 tctgcatcag aaataccggg accatgatcc gatattttaa cccacacatt acccgaacct
     8161 gacaattcta ctttcacctg attatctttc gaaaatttgc atccattatc cattatgttc
     8221 tgcaaggctg ttttaagcaa ttgttcattg ccttgtatct ccaggtgttc ggtttcatca
     8281 ggcaattgat tataaactac atttatcttg taatgaggaa aactccgaat aatggccgaa
     8341 cgcgcttccc atatcacttc atccagccgg atggtctcaa attctacctg gttgatgttg
     8401 ttctttatcc tggccaactg catgagctgg tctgacacta cactcaagtc acggacgtct
     8461 tccagtaccg agctgagtgt ttttctataa tcttcattgc tccgctcctt tgcaagcacc
     8521 acttctagct gggaagtgat gaccgtgagc ggattcttca actcatgtga gatattagac
     8581 aaaaagcttt tctggtgttt gaatgctgtt tctatcctgt ccagtagttt attgaaagta
     8641 tcacccaaac gccataattc atccttgttg ccggtggtct gcaatctctg actaagatct
     8701 gatggtaata ttttatccaa ctggttcatg atattggtga ccggcctaag agcattgcct
     8761 gcaaacagcc agcccgaaac agtcagaata atgataataa ggcaaaaatc tatgatgatg
     8821 atattgcgca gattagccag atcagttgaa ttgaatacag cttcggatat cacggtgtat
     8881 tctttgtcaa atttgtttgt ataaaatata ccgatagaat taaactttcc gtgagtaaag
     8941 cggtactctc cggagttctt tacctgttcg agtgtttgta ccggtatttc attttctttc
     9001 agtttgttaa attcgtatat ttttttgttc tcgtggttga aaatgagaaa attctccttt
     9061 gactccagat aatattctgt tttgtcagat gcggtttgtt ctgtaggctc tacattttga
     9121 atatttacca gcatttcggc cgtcatgtag gctttagact tcagatcgtt ataaaattgg
     9181 tcttctacat ggtccttaaa tttgaaataa ataaaaaaaa gtgatagttg aagtattatc
     9241 actacgatga gaataaattg tagcgtgagc cttgtgcgta tctgcattga tcaatcctgt
     9301 tcctgatgaa agatgtagcc catcccaaag cttgtgtgaa ttaatttttc gtcaaaacct
     9361 ttgtcaatct tgttgcgcaa ataattgaca taaacctcaa ccaggttagt accggtatca
     9421 aaattaatat cccagacaca ttcggtaatt tcctgcttgg ttatgacctt ttctttgttt
     9481 ctgataaaat attccaacag ggcaaattcc ttaggtgtca atttaataat cttttccttt
     9541 ctctttactt ttttggtctt caaatcaatt tctacatcat gataacgtag tatgtggcct
     9601 gcctgcaatt catctatccg acgtcgggtg agtgccttga tgcgggcgag tagctccctg
     9661 aattcataag gtttggccag gtaatcgtcg gccccggcat ccagaccaac tattttgtcc
     9721 tcggtagtgt cgagtgccga caccatgatg atgggagtct tgatgcctat atttctgaaa
     9781 tgcctgcaca ggtcgatacc attggtgtgt ggcaatatta catcacttat aatagcatca
     9841 tatttattgc gctctgccag aagtttgccc gtataaccat cataagcaaa atcgacaaca
     9901 atgtgatttt cttcgagacc ctgcttgatg gactgaactg tttttggatg atcctcaata
     9961 attaatattt tcatagaagc tgcttactaa ttataaaatg ggaatcataa tgtgtgcagt
    10021 ctctttttga acaaatgaat ttttaaaaaa attaacaatt tttaaattgt tcttcaaagc
    10081 cttacacatg atgccatttt atatattacg aaaaaaattg ctaatgttca gaaaaatggc
    10141 aaaaaactca aaaatgaagc tattcaccac atgaaaagtc ggaaaatgtg attaacagta
    10201 caaaaattga acaaataaac ccacaaacga tatgatacac tgactctgca aatgcatata
    10261 aaaaaattgg gcagaacagc ccattgtatc caaccggaca ttctctgaat tttgtatctt
    10321 aattaaacac ataagatgaa aacattacat tattttcaaa ttgctacact tgcttttctt
    10381 ttggtatttt cggcatgtag caaggacaat ggcaccaacg atcccggcac cccttctact
    10441 ggtgatccgg tgacactaca aggtgtgaat tatgaagcaa gtggtactgt caagtatgtt
    10501 gacaccaaca ccggcaaaac tgaaacaatg acttatacca aagtcacagc tcaatacaag
    10561 gaccaaagtc atatctggcc gggtacattt tacacttttc ttacttttac caatttaaca
    10621 cccaaccaga ctatatcatt tgtattccct ggcaaagagt tacctaaatc gggtatccac
    10681 aaaataggcc cgtggccaat agcaaccaat ggaatcaccg aagcagataa attaaaagat
    10741 ggagagatgg caatattttt caatggcaga ttagccggaa gcaaaagagc tgattttaaa
    10801 agcatcaatg tgttgaatga caatggaaaa attttgcttg attttggcaa tgaagttgat
    10861 gtttttgata acatcaccgg tgataaagca ggcaatatta ttttaataaa cgttaccaaa
    10921 accacaaaga aactataact ctgagacttt taaggctgtt gcagacatcc atacaacctc
    10981 aatgccaact ttgtatcaat ctacaaatta taatcctcgg catggtgtgc aaccagtcat
    11041 gaagcggcac tataatgtga tgaccattat ataagtgcat tcacctatta ccaaaagcct
    11101 gcaatacatg caggcttttt tgctttccag gcttttagaa tatatctttg gatcaagatt
    11161 gtttgacaca tgaaatttaa ttgcttattt tgggttattt tgacagcttg tgtgtcttgc
    11221 aatgaccatt caattcatgt taaaaaatcg aactctgcct cagaaataac cattaatcag
    11281 gcatatcagc aaatggtatt gagaaacgat ttacctgctg acagtatgga gtattatttt
    11341 cgtagccttg aaaatatgac aaccgatatt ccacaacagt tagcaatcaa gaatctgtgc
    11401 aggggcgcat atttccgcaa aaaagcttca tttgagcttg ctatttcata tttgaacagt
    11461 acactcggct acctgtcttc aaatgacagc ctcacttcag aagcattgct cggattgggt
    11521 atttgctaca aacatttagg tgattttcca aaagccctta gttatttcca aaaaagtgta
    11581 cagattagtg aaaacaacaa agataagcac gggcttgcaa gcacttttgc cgccatggca
    11641 caaatgtatt ttgaaaaaaa tgacattaaa cgatcaagag agttcataag taaagttttc
    11701 gagctgtaca acaatcacca aacaggtgta gcatatttca tagcattgca tacactcgcc
    11761 aatattgaag ggcagagcaa taattttgca aaagcactgg aaatagataa acaaggcata
    11821 gaactcgcca tcaaatatgg caacgatgct gttaaggttt cttttcagga caacatggcg
    11881 cggtgctatc tttacggata ccaggattac gaaaaagcac tttattactt caacaaaaac
    11941 cttaaaattg ataaaaaact aaacaacccc aactggatag ccgatacata tatcaatctg
    12001 gcagaagtag aaacagcaag aaaagatttt cgaagtgccg aaaaatccct gaaacaggca
    12061 attgacatct cattacagag taagcagttt atcaatattt caaaagcata caaggctctc
    12121 agcgaactgt ataaaaccca aggtaggtac gacaaagcac tcagtgcaaa cgaagcatat
    12181 accaaacatt accagaatgt gattaatgaa aaaagtgaac aggccttcgc acaatacaat
    12241 ttactgttcg aaacagaaaa gaaagaaaaa gaaatagcag aaaccaaact caaatcaaaa
    12301 cagaagaata tttggctaat attgctgaca ggtgggttgc tgataggaat ggtcatttac
    12361 cgggcacaaa gaaacaaatc aaaacacaaa caacaccagc tacttttaga aaacagactg
    12421 ctacaagagg aagcacaaat acaaatgcaa caacaacgcc tggagatatc aagagatctg
    12481 cacgacagcc tggggtcgca acttacactg atgaatactg tgttggacag catcaaagca
    12541 gatagccggc tcgatgaaaa aacaaatagc aaaataagaa aactatcaga cttctctgaa
    12601 gattctatat ccgaactcaa aagtgtgctt tgggtactca ataccaacga aatacggatg
    12661 aaagacctta aatacaaact gttaaatctt ataaaaaatg ccggagaagc caaggaagat
    12721 atgcagtttc tgttaaatat gaatgtaaat gaaaataaac ccctgtcggg aaaacaagct
    12781 ataaatattt ttagggtagt gcaggaaatc gtcaacaatg ctatcaaata ctcagaggct
    12841 gaagtaataa aaatcaacct gtttgaagaa aacaatgctc tatgcctgaa tatttccgac
    12901 aatggtaatg gctttgatct ggagactgaa aaatccaaat cattcgggct taataatatc
    12961 caataccgca tagaagaaat aggaggtttc gtaaaaattt cttcatctgg tggcacaact
    13021 tttaacattc aaataccatt aaaatcatga ttcggattgg acttgtagaa gataagcctt
    13081 tttctttaaa agctttggaa gaaaagctaa gcagatataa tgatttgcat atagtattta
    13141 cggcaaaaaa cggcttcgat gctattgcca aactcgctga ccacaaagaa accgatctga
    13201 tacttatgga catcgaaatg cccgacatga acggtataga agcaaccggc atcatcaaac
    13261 aacaatacaa aaacatcaaa atagtgatga taaccatcta tgatgacgac aattacatct
    13321 ttgatgccat acaagccgga gccgacagtt atattcttaa agaaaccaaa gccgaaaaaa
    13381 tatatgaaac cattcaggat acactcaatg gcggagcagt catgtcacca tcaatagcgg
    13441 taaaagcatt gcaactgctg aaaattaacg gaaatgtacg aagtgacgat accactcctg
    13501 aaatcattct gtcagaccgt gaaactgaaa tactggaaca acttgccaaa ggcctaacca
    13561 ataagcgaat tgccgaaatc ctgttcatct caccttttac agtcaagcga catatagaaa
    13621 acatctacca gaaactacag gctcacaaca gaatagagct ccttgataaa gccaggaaaa
    13681 ataatctgct ttaaacccgg ataacaattg acagcctgac agcagagtat tacattttat
    13741 ttcacttaca aaaaatacaa tgccccttac tcttgtattt ttact
//
LOCUS       DAOQQY010000040        13726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1492_length_13726_cov_6.871699, whole genome shotgun sequence.
ACCESSION   DAOQQY010000040 DAOQQY010000000
VERSION     DAOQQY010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13726
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1492_length_13726_cov_6.871699"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /locus_tag="PLE23_03690"
     CDS             complement(<1..1964)
                     /locus_tag="PLE23_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HMX82398.1"
                     /translation="MLTLIFLLGIVSLTNATTSFAILGKDFKNNSTTSVNNSIVACDI
                     EYTANVVHNKCYNDNSGSISLNVTGGTPGYTFQWSNGASGNPISNLAAGNYSVTIADT
                     QGCTKTGTIKVNNGPLIFVKAWIENADCYTGANGSIVLNVNGGAIPYTYLWSNGATTK
                     DLINIPAGNYSVTIKDANGCTITKNYTVTQNSDLQVTGVVNHPSCQHPNSGSIIQTVT
                     GGTSPYNYQWSNGALSKDLVNIPDGSYSVTVTDITGCSVVKNYIVLSPSDINISGTIS
                     NASCGLNNGSINITVSGGTPGYSYAWSNGSSAEDQNNLAAGSYTVTVTDSKGCTKTAS
                     FTINSTNGPSANGTVTNVSCYGGNDGAIALSVSGGTPGYSYNWSNGASSQNISALAAG
                     NYSVTVTDANGCTVVKSFTVTQPAQIQIAAQTVNASCGLNNGSINITVSGGTPGYSYA
                     WSNGSSAEDQNNLAAGNYTVTVTDSKGCTKTASFTINTTNGPSANGTVTNVSCYGGND
                     GAIALSVSGGTPGYSYNWSNGASSQNISALAAGNYSVTVTDANGCTVVKSFTVTQPAQ
                     IQIVAQTVNASCGLNNGSINITVSGGTPGYSYAWSNGSSAEDQNNLAAGSYTVTVTDS
                     KGCTKTASFTINSTNGPSANGTVTNVSCYGG"
     gene            complement(2402..2992)
                     /gene="coaE"
                     /locus_tag="PLE23_03695"
     CDS             complement(2402..2992)
                     /gene="coaE"
                     /locus_tag="PLE23_03695"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMX82399.1"
                     /translation="MKLIGITGYIGSGKSTVAKILGFLGALIYDSDAAAKRLMHENQD
                     LIKALTDRFGKDIYDEKLCIRTEKLRQLVFGNEALRTALNNIVHPFVYLDFDDFKQKN
                     SEAEIIVFESAILLKGSNYNQFHSIIFVTSQKKLLYSRIMQRSKLTKELIDKILATQR
                     VKLSDFNNNCIIRISNNEKHLLIPQIVKIFNSIKNL"
     gene            complement(2989..3639)
                     /locus_tag="PLE23_03700"
     CDS             complement(2989..3639)
                     /locus_tag="PLE23_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HMX82400.1"
                     /translation="MRNEITSYQTINNRVIAEFKDKGSKFFAISFPVANKHEFDLELA
                     AIKDEFPKATHYCYAYKIGHDGNNFRINDDGEPSGTAGKPIFSQIESNKLTNIAIVVV
                     RYYGGTKLGVPGLIHAYKSSAAISISENTVIVKIIEKNFLIKFDSSQYKQMQVCIGQL
                     KKISSDISFISTLEVAVNVPLESIENLKKIVFENTFGYAYEGSSSALLEHILIETE"
     gene            3790..5274
                     /locus_tag="PLE23_03705"
     CDS             3790..5274
                     /locus_tag="PLE23_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMX82401.1"
                     /translation="MGIIQLLIYFAIAGLLLTLITRYITKQHGTWLMSFLQHFAGVWF
                     LVSGFVKAVDPLGTAYKMEQYFAEFESTFQHTWVSFITPMFPLLGKYSISFSVFMIII
                     EMVLGILLITGGWKKFTGWLFLIIVVFFTILTGFTFLTGYVPEGANFFQFSKWVAYDA
                     TRMKVTDCGCFGDFLKLEPKVSFMKDLFLLIPSVLFIIRRSQMHTLINDKFTKWYVGG
                     ITALLLFYCLQNFYFDIPTIDFRPFKEGVNVRETKLAEEKAASEVQITAYKLKNLKDG
                     KIVEIPTAQYLAEMTKYPKEEWEVVDQVKSELAVPHTKISEFSFNNTEGEDIAESLLA
                     EKSYSIMISAYKLGIKEINEKTVEVPDSIFSADTIRVGKDSIAVVKRFEKIEMKKTDQ
                     RSFVWDEDYLNIYKNIIVPFVKEAEKNGIKCYMVCNSYPDAIKDFRKQTGFDADIYTG
                     DDLLIKTIIRSNPGITLWKDGIILKHFHYKKLPEFNQFKQAYIK"
     gene            5292..6227
                     /gene="nusB"
                     /locus_tag="PLE23_03710"
     CDS             5292..6227
                     /gene="nusB"
                     /locus_tag="PLE23_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HMX82402.1"
                     /translation="MLSRRNVRIKVLQVIYGSNINHDKIERYQRNYQTSIDRSLEILV
                     YSLRVMQKILKYSLTDAENRKYKKLPSDNDKLFKPVLLTNKQSQNLMNSQLLEIYTRK
                     FHTDTKIDQDVIRRLYLEFSKDEDYIQYYIAAEHTTEENIAMLLKIYKLLTKNEVFNE
                     TIEDHYVSWDDDESLVVGAMKKIIKSLDQDEYDFLKDYIPDDETVKEFGETLLIATVK
                     NDKSLVELITPFLENWELERVAAIDLIMLKMAVTEILRIESIPTKVTLNEYVEISKIY
                     STEKSKEFINGILDRIINKLNNENKIIKTGRGLDN"
     gene            6298..7713
                     /locus_tag="PLE23_03715"
     CDS             6298..7713
                     /locus_tag="PLE23_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82403.1"
                     /translation="MKKLFYTLILIAMLSANTGIAQFKTAPDGVTAKRVLMDFYTPAN
                     DFDPITSLRDLKDLGGAEIGYNRHLASWLNLAVPLRIGVADMPNGTGGFKPRVLNTSL
                     DAVLQIKYFKPKKLLAPYLMAGVGAAMENWENTRVEIPIGVGLNVRIAPLVYINAQTE
                     YRLALKDDRNNLAHSLGFLFLIGNEINDRDKDGIADEVDECPDEFGLAQFNGCPDKDG
                     DGIPDKTDKCPEQPGTEATMGCPDADGDGIADGDDKCPDQAGPKATMGCPDRDGDGVA
                     DKDDDCPDVAGLKSLKGCPDKDGDGIADAKDKCPNEAGPAATMGCPDRDGDGVADKDD
                     KCPDQFGPASNQGCPEMKKEEKAVLENAARSIQFEFGSAVIKKESYAILDQVVGLMKT
                     YPTYSCDISGHTDNVGNSAVNQSLSEKRAKACLDYLVSKGVEAKRMTSAGYGDTKPVG
                     DNKTSQGRKLNRRTEFLMSVK"
     gene            7799..9340
                     /gene="guaA"
                     /locus_tag="PLE23_03720"
     CDS             7799..9340
                     /gene="guaA"
                     /locus_tag="PLE23_03720"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMX82404.1"
                     /translation="MKEKILIFDFGSQYTQLIARRIREADVYSEILPYNADYTLDESI
                     KGVILSGSPFSVNDENFLDFNVEKLNGKIPVLAVCYGAQLMAKKLGGSVSKSSKREYG
                     RATLNITKPEDKVLSGLSANSQVWMSHGDTILQIPEGFELLAYTDSIPVAAFRSSTSL
                     FTNPVYCFQFHPEVTHSVEGHLLIRNFLFDVCGCSGDWTPEHFIESTINDLKQKIGSE
                     HVLMALSGGVDSTVAAELLHKAIGNQLHCFFLDNGLLRKNEFQSVLKSYENMGLNIQG
                     IDSSNEFYTALNGLSDPEDKRKAIGRVFIEVFEKYAKTLPEVKWLGQGTIYPDVIESV
                     SVHGPSVTIKSHHNVGGLPEKLHLKIIEPLRALFKDEVRRVGKALGIPAIILNRHPFP
                     GPGLGIRILGDITPAKVKLLQEADDIFISNLRESGYYDKVWQAGAVLLPIYSVGVMGD
                     ERTYEQVVALRSVNSVDGMTAEWSRLPFDLLAKISEDIINNVRGINRVVFDISTKPPA
                     TIEWE"
     gene            9368..10774
                     /locus_tag="PLE23_03725"
     CDS             9368..10774
                     /locus_tag="PLE23_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMX82405.1"
                     /translation="MNKILYSLALILVLGTGCSSVKSAQSTNSGKIKKEVSKDESSVV
                     YNPETKKYEKVADKPTDTILPPATKKDTVMKKEVIVKKDTQLVMPNKTLLSSNLRESN
                     RKSTYNVSLVLPFNTGDSQNSSTNLSRNSVWAVHFYSGAKLALKKFENTGVKINLNVI
                     DTKNLETSISSLLNRPELLNADLIIGPYSSSQAKILIDFVKDRKTTLVSPYTTSSNPT
                     TANPNYIQANPSIYRHFEALSKHIAANFSADQIVFLTQNTESAKSRTQNFINTLNSFL
                     TPEAKSKIREIVLPDNFLLSSEVKLDSTVTRNNETVFIVPSWDERYVKDVLKKIENGR
                     DGVPVYVYGMPQWQNMDDLKYLMTNLNARISSNVLLNLNNSNVDKFRSEYFDTYAKMP
                     DLEAFSGYDVTSYFFSRLVSQGDFFQKDSRSAEKELYTRFDIKPVMNDQQPGIVDYFE
                     NYGLQIIQIKGSGFDILD"
     gene            10779..12137
                     /locus_tag="PLE23_03730"
     CDS             10779..12137
                     /locus_tag="PLE23_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMX82406.1"
                     /translation="MFISQNPVTSEVLGRYESLNDKDLSSLLKDAHTAHNELKGVLVT
                     ERCLMLKRFRILLNNNKELLAQTITAEMGKPITQARAEIDKSVLLCDFYAENVEKLLE
                     PKTFLAKPFSVKLRHEALGIILGIMPWNFPVWQSLRFAIPAIMAGNAVLLKPAPNTAL
                     SVLMLEGFFSESFTIQVFKTVLSDTTLVPKIIADPRVQGVSLTGSDKAGSKVGALAGK
                     YLKKSVLELGGCDPFIVFPDADVMLAAKLAVHSRINNTGQTCIAAKRLLVHKDIYKEF
                     KDYLIHLFEELKMGDPIQESTQVSSLARADIRDILHVQYLDALAKGAKAIVEGGLVQG
                     NGFYYNPALLENTNKKMLVRRQEVFGPLLTIQSFKDARDAIDQANDNRYGLGASIWTQ
                     NASLQQQMESKLQVGYIVFNNFVSSDPRIPFGGIKQSGYGRELGDAGIHEFTNLKSIL
                     YS"
     gene            12134..12802
                     /locus_tag="PLE23_03735"
     CDS             12134..12802
                     /locus_tag="PLE23_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMX82407.1"
                     /translation="MKVNKRRQELISEIVSKRQLDLCVVLENVQDDHNIGAVLRTCDA
                     VGIQELYILNTEPLIKKRFVKVGKRTSAGTRKYLNVFYYNDPEACFSHIKSKYKRILG
                     TSLGEKSVSLYKLDLTESAAFVFGNEKTGITPGTQPYIEQNFLIPMNGFAQSLNISVA
                     CAISLYECYRQREQKGMYTDAIQNDKQELLQHYLERSRGKVNPSVDYKIFSSDTDNKL
                     AGNK"
     gene            12814..>13726
                     /locus_tag="PLE23_03740"
     CDS             12814..>13726
                     /locus_tag="PLE23_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX82408.1"
                     /translation="MRLQKLEIKGFKSFANETTVHFHENVTGVVGPNGSGKSNIVDAF
                     RWVLGEQKSKDLRLDKMSSVIFNGTSKKKESALAQVSLTFDNNKGILPSEYNSITITR
                     MLYRNGDSEYRINDVTCRLKDITTLLLDTGMGSDSYAIIALNMVDDLLADKENSRQRM
                     LFQASGITKYKVRKEETLRKLKSTEDDLDRVKDLLFEIETNIKSLEKQAKRAEKFFTI
                     KDAYKDQSLKLAIHQVSRTKAQFKQLSQQIEKEEDNYRAAEVSSRTLEAKLEQTKKSN
                     LDKEKFLTEKQKETNELLSRIRSLENEK"
BASE COUNT         4474 a         2498 c         2750 g         4004 t
ORIGIN      
        1 ccaccgtagc aagaaacgtt ggtaactgtt ccgttagcag aaggtccgtt ggtagagtta
       61 atggtgaaac ttgccgtttt cgtacaaccc ttcgaatcag taactgtaac cgtgtagcta
      121 cctgctgcga gattattctg gtcttcagca gaagaaccat tcgaccaagc ataagaatag
      181 cctggcgtgc cacccgatac agtaatattt attgatccgt tgtttagtcc gcaagatgca
      241 tttacagtct gcgctacaat ctgaatctga gcaggctgag taacggtgaa tgattttacc
      301 acggtacaac cattggcatc tgtaaccgtt acggaataat taccggcagc gagagctgaa
      361 atattttgag aagaagcgcc attgctccaa ttgtatgaat aacccggagt gccacctgaa
      421 acgcttaatg caatagcacc atcattgcca ccgtagcaag aaacgttggt aactgttccg
      481 ttagcagaag gtccgttggt agtgttaatg gtgaaacttg ctgttttcgt acaacctttc
      541 gaatcagtaa ctgtaaccgt gtagttacct gctgcgagat tgttctggtc ttcagcagaa
      601 gaaccattcg accaagcata agaatagcct ggtgtgccac ccgatacagt aatattaata
      661 gatccgttgt ttagtccgca agatgcgttt acagtctgcg ctgcaatctg aatctgagca
      721 ggctgagtaa cggtgaatga ttttaccacg gtacaaccat tggcatctgt aaccgttacg
      781 gaataattac cggcagcgag agctgaaata ttttgagaag aagcgccatt gctccaattg
      841 tatgaataac ccggagtgcc acctgaaacg cttaatgcaa tagcaccatc attgccaccg
      901 tagcaagaaa cgttggtaac tgttccgtta gcagaaggtc cgttggtaga gttaatggtg
      961 aaacttgccg ttttcgtaca acccttcgaa tcagtaactg taaccgtgta gctacctgct
     1021 gcgagattat tctggtcttc agcagaagaa ccattcgacc aagcataaga atagcctggc
     1081 gtgccacccg atacagtaat atttattgat ccgttgttta gtccgcaaga tgcattcgat
     1141 attgtaccac tgatattgat atcagatggt gaaaggacaa tataattttt taccactgag
     1201 catccagtga tgtctgtgac tgtgacagaa tacgacccat ccgggatgtt taccaagtct
     1261 ttgcttaatg cgccatttga ccattgataa ttataaggtg atgtaccgcc agttactgtt
     1321 tggatgattg aaccactatt cggatgctgg caacttgggt gattaacaac tccggttacc
     1381 tgtaaatcgc tgttttgagt gacagtataa tttttagtta ttgtacatcc attagcatcc
     1441 ttaatggtta cagaataatt gccagctgga atattaatga ggtctttagt agtagcacca
     1501 ttactccaaa gataggtgta tgggatagca ccgccattaa cgttcagcac aattgaacca
     1561 ttggcaccgg tatagcagtc tgcattttca atccaagctt ttacaaatat gagaggcccg
     1621 ttgttgactt taatggtgcc tgtcttggta caaccttggg tgtccgctat agtaacagaa
     1681 taatttcctg cagccaggtt cgaaattggg ttgccggatg ctccgttcga ccattggaaa
     1741 gtgtaaccag gcgtaccacc tgtgacattg agtgatatcg aacctgagtt atcattgtag
     1801 catttgttgt gcacaacatt agcagtgtat tcaatatcac aagcgacgat gctattgttt
     1861 acacttgtag ttgaattatt tttaaaatct ttgcccaaaa tagcaaaaga tgttgtagca
     1921 tttgttaatg aaacaatgcc caataagaat atcaatgtta acattgaccg agaagtatca
     1981 agcctcatgg ttgttttttt taagttaact aattttaagg acttcgaggc agttacgatt
     2041 tttgagaaaa tgtcggattt tgggtgagaa ttagccttat tcaaaatgaa taatgacaga
     2101 tgttattaac atatttcaaa atttatgcct aattttaata tattaattca cattcttcac
     2161 ttgggcaaac aactttcaca aacaattctt tttgtaatgt tactgttttg tttattcaca
     2221 taaataaaaa gaatcatacg aaacaacctc gttgcgtatg gatattttta acattttcag
     2281 taggtcttag ggtgaaaatt tgattttggg gatcatgtgc aaatttagat tatgaactcg
     2341 gatttttcta tttttttttt gaaaacacct gtcattacta atttttcaaa cctgaattgt
     2401 gtcacagatt ctttatagaa ttaaagattt tcactatttg tggtataagt aagtgttttt
     2461 cattattact tatacgaatg atacaattat tattgaaatc agacaatttt acacgttgag
     2521 tagcgagaat tttatcaatc aattcttttg tcaatttgct acgctgcatt attcttgaat
     2581 ataataattt tttttgtgat gtcacaaaaa ttatggaatg aaattgatta taatttgaac
     2641 ctttgagcaa aatcgcagat tcgaacacaa ttatttctgc ttcagaattt ttctgtttga
     2701 aatcatcaaa gtcaagatat acaaaaggat gaacgatatt gttaagtgct gttctcaatg
     2761 cttcattgcc aaataccaat tgtcttaatt tttcagtacg gatacatagt ttttcatcat
     2821 aaatatcctt tccgaatctg tcggttaaag ctttgattaa atcctggttt tcgtgcatca
     2881 ggcgctttgc ggctgcatca ctgtcataaa taagagcacc caggaaccca agaattttag
     2941 cgacagttga tttgccgcta cctatataac ctgttatacc gataagtttc attctgtttc
     3001 tattaatatg tgctcaagca atgctgacga cgaaccttca taagcatagc caaaagtatt
     3061 ttcgaagact atttttttca aattttcaat actttccagc ggaacattta cagcaacttc
     3121 taaagttgaa atgaagctta tgtcagagct tatttttttt agctgaccaa tacacacctg
     3181 catttgttta tattgtgatg agtcaaattt gataagaaag tttttctcaa taatttttac
     3241 aatgacagta ttttctgaaa tagatatagc tgcagatgat ttgtaggcat ggataagacc
     3301 tgggacacca agttttgtac caccataata tctgaccaca actattgcaa tattagtgag
     3361 tttgttagat tcgatctggc tgaatattgg ttttccggca gtaccggatg gttcgccgtc
     3421 atcgttgata cggaaattat tcccgtcatg tccgattttg tatgcataac aataatgtgt
     3481 agctttgggg aattcatcct ttattgcagc aagttcaagg tcaaattcat gtttattggc
     3541 tactggaaag gagatagcaa agaacttgct tcctttatct ttgaattcgg caataacccg
     3601 attgttgata gtctgatatg aagttatttc gttacgcatt catgtaatgt cttggaattt
     3661 atgttctgcg gagtttgaac ttaaagtgca aatttgagtt aaaatacaat agaatcataa
     3721 actgaagttg atttaactca taaaaagtga taaaaatcat aaatttgcat ttcaattaat
     3781 tttcaagaca tgggcatcat acaattattg atttattttg caattgcagg tctcttactt
     3841 actttaatta ccagatatat aaccaagcaa catggtactt ggttaatgag ttttctccag
     3901 cattttgcgg gtgtatggtt tttggtgtcc ggttttgtaa aagctgttga tccgcttggt
     3961 actgcctaca aaatggaaca atattttgca gaatttgaaa gcacatttca acatacatgg
     4021 gtatcgttca ttaccccaat gtttccactg cttgggaaat attcgatttc attttcagtt
     4081 tttatgatta ttattgagat ggtacttggc attctactca taacaggtgg gtggaagaaa
     4141 tttacaggat ggctttttct cattattgtt gtgtttttta ctatacttac cggttttaca
     4201 tttcttacgg gttatgtacc cgaaggtgcc aattttttcc agttttcaaa gtgggtggcc
     4261 tatgatgcta ctcgtatgaa agttacagat tgtggttgtt ttggtgattt tctcaagctc
     4321 gaacctaagg tttcatttat gaaagacttg ttcctgttga ttccgtctgt gttgtttatc
     4381 attcgcagaa gccaaatgca tacgctcatt aatgataaat ttacgaaatg gtatgtaggg
     4441 ggcatcactg ctttacttct gttttattgt ttacaaaatt tttattttga tattcctaca
     4501 attgacttca ggccttttaa agagggtgtt aacgtaagag agacaaaact cgctgaagaa
     4561 aaagctgctt ctgaagtaca gattactgct tacaagctga aaaatttaaa agacggcaaa
     4621 atagttgaga ttcctactgc gcaatatctt gcagaaatga ccaagtaccc taaagaggaa
     4681 tgggaagtag tggatcaggt aaaatcagaa ctcgcagtac cacacacaaa aataagcgaa
     4741 ttttcattta ataacactga aggcgaagat attgccgaga gtttgcttgc tgaaaaatca
     4801 tattcgatta tgatatcagc ctacaaatta ggaatcaaag aaataaacga gaaaacagtt
     4861 gaagtcccgg atagtatttt cagtgcggat accatcagag tcggtaagga ttcaatagct
     4921 gttgtcaagc gttttgaaaa gatagaaatg aaaaagacag atcagcgaag ttttgtctgg
     4981 gatgaagact accttaatat atacaaaaat attatagtgc cttttgtaaa ggaagctgag
     5041 aagaatggta ttaaatgcta tatggtctgc aattcctatc ccgatgccat taaagatttt
     5101 agaaagcaaa ccggatttga tgccgatatt tatacaggtg atgatttgct tataaagacc
     5161 atcatcaggt cgaacccggg tataacttta tggaaagatg gaataatact caaacatttc
     5221 cattacaaaa aattgcctga atttaaccag tttaaacaag cttatattaa ataacaaaaa
     5281 ccgatcagag aatgttgagt agaaggaatg tgcggataaa agtgttacaa gtcatctatg
     5341 gcagtaatat aaaccatgat aagatagaaa gataccaaag aaattatcag acgagtatcg
     5401 accgttcgct ggagatattg gtttattctc tgcgagtcat gcagaagata ctaaaatatt
     5461 cactcacgga tgcagaaaat cgcaagtaca aaaaattacc ttctgacaac gataaattgt
     5521 tcaaacctgt actactgacg aacaaacaaa gtcaaaactt gatgaacagt cagttgctcg
     5581 aaatttatac acgaaaattt cacacagata caaaaataga ccaagatgtt atacgcagac
     5641 tatatcttga gttctcaaaa gatgaagatt acatacaata ttatattgct gcagaacata
     5701 ctaccgagga aaacattgca atgcttttga agatttacaa attacttaca aagaatgaag
     5761 tgtttaatga aactatagaa gaccattatg tatcatggga tgatgacgaa tcgcttgtag
     5821 ttggcgctat gaaaaaaatc atcaaatcac tcgaccaaga cgagtatgat tttctcaaag
     5881 attatattcc tgacgacgaa actgtaaaag aatttggtga aacactgctt atagcaactg
     5941 ttaaaaatga caaatcattg gttgagctca taacaccatt tcttgaaaac tgggagcttg
     6001 aaagggttgc agctatagac ctcatcatgt taaaaatggc agttactgag attctccgca
     6061 tcgaatctat acctacaaaa gtgacactca atgaatatgt cgaaatctca aagatataca
     6121 gtactgaaaa aagtaaagaa tttattaatg gtatcctcga ccgtattatt aacaaactca
     6181 acaatgaaaa taaaattata aaaacaggtc gcggactcga taattaaaaa ttattacttt
     6241 tatttctaat atttaatcat tgttatctac atttgcataa ataaactttg attaagcatg
     6301 aaaaaactat tttacacatt aattttgata gcaatgctta gtgccaatac tggtattgct
     6361 cagttcaaaa cagctcctga cggtgtcact gccaaaaggg tgttaatgga cttttatact
     6421 cctgccaatg attttgatcc catcacatca cttcgtgatc ttaaagatct tggaggtgca
     6481 gagataggat acaacagaca cctggcaagc tggctaaatc tggcagttcc gctccgtatt
     6541 ggtgtagctg atatgcctaa tggcactggc ggattcaagc caagagtatt gaataccagt
     6601 ctggatgcag ttttgcagat taaatatttt aaacctaaga agttacttgc cccttacctc
     6661 atggcaggtg taggtgcagc aatggaaaac tgggaaaata ccagagtaga aattccaatt
     6721 ggtgttggtc ttaatgttag aattgctcca cttgtgtata tcaatgccca aacagaatat
     6781 cgtctggctt taaaggatga cagaaacaat cttgcacata gtttaggatt tttgttcctc
     6841 atcggtaatg aaatcaacga ccgtgacaag gatggtatag cagacgaagt ggacgaatgt
     6901 cctgacgaat ttggtcttgc acagttcaat ggttgtccag acaaagatgg tgatggtatt
     6961 cctgataaaa cagataaatg ccctgaacag cctggtactg aggctacaat gggttgcccg
     7021 gatgctgacg gtgatggtat agctgacggt gacgacaaat gcccggatca agcaggtcca
     7081 aaagctacaa tgggttgccc tgatagagat ggcgacggtg tagcagataa ggatgatgat
     7141 tgcccggatg tggctggtct taaatcattg aaaggttgcc cggacaaaga cggagacggt
     7201 atagctgatg ccaaagacaa atgccctaat gaagccggtc cggcagctac tatgggttgc
     7261 cctgacagag atggtgacgg agtagcagat aaagatgata aatgccctga tcaattcgga
     7321 cctgcatcta accaaggttg cccggaaatg aagaaagaag aaaaggccgt acttgaaaat
     7381 gccgcaagat ctatccaatt cgaattcggt agtgccgtta tcaagaaaga gtcttatgct
     7441 attcttgatc aagttgttgg tttgatgaaa acgtatccta cttacagttg cgacatttca
     7501 ggtcatactg ataatgttgg taattctgct gtaaatcagt cgctttctga gaaaagagca
     7561 aaggcatgtc ttgactatct tgtatcaaaa ggtgtagaag ccaagagaat gacaagtgcc
     7621 ggctatggcg acacaaagcc agtaggcgac aacaagactt cacaaggacg aaaattgaac
     7681 agaaggactg agttcctgat gagcgttaaa taaaattaac aataaagtaa agaatggctt
     7741 gtggatttct acaagccatt ttttattgta gaaaatgaga tataactata tttgcaccat
     7801 gaaagagaaa atattgatat ttgattttgg gtcacaatac acacagctta ttgcaagacg
     7861 cataagagaa gccgatgtgt actctgagat tttgccttac aatgccgatt atacacttga
     7921 tgaaagtata aaaggagtga tactttccgg tagcccgttt tcagtaaatg acgaaaattt
     7981 tttagacttt aatgtcgaaa aactcaatgg caaaataccg gtattggcgg tatgttacgg
     8041 agctcaactt atggccaaaa aactaggtgg tagcgtttcg aaatcatcca agagagaata
     8101 tggtcgtgct acattaaata ttactaaacc agaggataaa gttttatcag gattaagtgc
     8161 caattcgcag gtctggatgt ctcacggcga tacaattctc caaattccgg aaggattcga
     8221 attactggct tataccgatt cgattccggt ggctgccttc agatcttcta cttcactctt
     8281 tacaaaccct gtatattgtt ttcagttcca tccagaagta acccattcgg ttgaagggca
     8341 tttacttatc cgcaattttt tgtttgatgt ctgtggatgt tccggcgact ggacacccga
     8401 acattttatc gaaagtacca tcaatgatct aaaacaaaaa attggcagcg aacacgtatt
     8461 gatggcattg tcaggtggtg ttgacagtac tgtcgccgca gaattattac ataaagcaat
     8521 aggtaatcaa ttgcattgct tctttcttga taacggatta ttgagaaaaa atgaatttca
     8581 gtctgtctta aagtcttatg aaaatatggg actcaacata cagggcattg acagcagtaa
     8641 tgaattttac accgcattaa acggactctc agatcctgaa gacaagcgta aagccatcgg
     8701 tcgtgtgttc atagaagtat ttgaaaaata tgccaaaacg cttccagaag tgaaatggct
     8761 cggtcaaggc accatttatc cggatgtcat agaatcagta tctgttcacg gtccttcagt
     8821 tacaatcaaa tcgcaccaca atgtaggtgg cttgccagaa aaactccacc tgaaaattat
     8881 tgaaccgctc agggctttgt ttaaagatga agttcgacgt gtagggaagg ctttgggcat
     8941 acctgcaatt atacttaacc ggcacccttt tccgggcccg ggccttggta tcaggatact
     9001 tggcgatata actcccgcta aagttaagct gttgcaggaa gccgacgata tatttatttc
     9061 taatcttaga gaatcaggat attatgacaa agtctggcag gccggtgcag tattactccc
     9121 gatatactca gtaggcgtga tgggcgacga aagaacctat gagcaggtag tcgcgctcag
     9181 gagtgtcaat tctgttgacg gtatgaccgc cgaatggagc agactgcctt ttgacttact
     9241 tgcaaaaatt tctgaagata taattaacaa cgttcgtggt atcaatcgtg tagtgtttga
     9301 tattagtacc aagccgcctg ctactattga gtgggaataa atttttttaa attaatagat
     9361 tctgagaatg aacaaaatat tatactcatt ggcgcttata ttggtgctgg gtacaggttg
     9421 tagttctgtc aaaagtgccc aatcaactaa cagtggcaag ataaaaaagg aagtcagtaa
     9481 ggatgaaagc tcagtagtgt ataatccaga gactaaaaag tacgaaaaag tagcagacaa
     9541 acccaccgac actatcttgc ctccggcgac aaagaaagat accgtaatga aaaaggaagt
     9601 gatagtgaaa aaggatactc aactggtaat gcctaataaa acactgttgt cttctaactt
     9661 gagggagtct aaccggaaaa gtacttacaa tgtaagcctt gttcttcctt ttaataccgg
     9721 tgattcacaa aatagttcaa caaatctgag tagaaattcg gtgtgggctg ttcattttta
     9781 ttctggagct aaacttgcac ttaagaaatt tgaaaatacc ggtgtaaaaa taaatctcaa
     9841 tgtaatagat actaaaaacc tggaaacttc catcagcagt ctgcttaaca gaccagaact
     9901 gctcaatgca gacctaataa tagggccgta tagtagcagt caagcaaaga tattaataga
     9961 ttttgtcaaa gatagaaaga ctacactggt atcaccttat actacttctt ctaacccgac
    10021 aactgctaac ccaaattaca tccaggccaa tccttccatt tacagacact ttgaagcatt
    10081 gtcaaaacat atagccgcca atttttctgc cgatcaaatc gtatttctga ctcaaaatac
    10141 tgaatctgca aagagccgaa ctcaaaattt cataaacacg cttaactcat tcttaacacc
    10201 cgaagccaaa tcaaaaatac gtgaaattgt acttccggat aattttttgc tttcttcgga
    10261 agttaaactt gattcgactg ttaccagaaa taacgaaact gtctttattg tcccttcctg
    10321 ggacgaacgt tatgtaaagg atgtattgaa aaagattgaa aatggccgag atggtgtacc
    10381 tgtttatgtt tacggcatgc cgcaatggca gaatatggac gatcttaaat acttaatgac
    10441 aaaccttaat gcccgaatca gcagtaatgt gctacttaac ctgaacaaca gtaatgttga
    10501 taaatttaga agtgagtatt ttgatactta tgccaaaatg cccgatctag aagcattctc
    10561 cggatatgat gttaccagct atttcttttc acgtttggtc agtcaaggtg attttttcca
    10621 aaaggattca agaagtgctg aaaaagaact ctacacacgt tttgatatta aacctgtcat
    10681 gaacgatcaa caaccaggta tagttgatta ctttgaaaac tacggcttac aaatcattca
    10741 gattaaaggt tcaggtttcg atattttaga ctaacccgat gtttatttct caaaaccctg
    10801 ttacctcgga agttttaggc cggtatgaat cattaaatga caaagacctg agctcgttat
    10861 tgaaggatgc acatacggca cataacgaac tgaagggtgt tttggtaaca gagagatgtc
    10921 tgatgctaaa acggtttaga attttattga acaacaataa ggaacttttg gctcaaacca
    10981 tcactgccga aatggggaag ccaataacgc aggcaagagc agaaatcgat aaatctgttt
    11041 tattatgtga tttttatgcc gaaaatgttg aaaaattact cgagcctaaa acttttcttg
    11101 ccaaaccatt cagcgtaaaa ctcagacatg aagcattagg catcatactt ggcatcatgc
    11161 cttggaattt ccctgtttgg caaagtctca ggttcgctat accagccatt atggccggaa
    11221 atgcagtttt gttaaaacct gcccccaaca ccgcattatc tgtgttgatg cttgaagggt
    11281 tcttttccga gagttttacc attcaggtat ttaaaactgt gctgtcagat actacacttg
    11341 taccaaaaat aatagccgat ccgcgtgtac aaggagtatc tcttaccggt agtgataagg
    11401 caggtagtaa agtaggtgct cttgccggca aatacttgaa aaaatctgtg ctcgaacttg
    11461 gcggttgtga tccatttata gtgtttcctg atgcagatgt gatgcttgct gccaagcttg
    11521 ctgtgcattc aagaataaat aacacagggc aaacttgtat tgctgctaaa agattattgg
    11581 tacataagga tatttataaa gaatttaaag attatctcat tcacttattt gaagaattga
    11641 aaatgggtga cccaattcag gaatctactc aggtaagttc gctcgcaagg gcagatataa
    11701 gagatatact tcatgttcag tacctggatg cacttgcaaa gggagcaaaa gctattgtag
    11761 aaggcggact agtacaaggt aacggattct attataatcc tgcattgctc gagaatacaa
    11821 ataaaaagat gctcgtgcgt cgtcaagaag tattcggacc tttacttact atccaaagtt
    11881 tcaaagatgc ccgggatgca atagaccaag ccaatgacaa caggtacggt ctgggtgcaa
    11941 gcatttggac ccagaatgcc agccttcaac agcaaatgga atcaaaatta caagtcggat
    12001 atattgtatt taataacttt gtgagttcgg atcctcgtat tccgttcggt ggcataaaac
    12061 agtcaggtta tggaagagag ttgggtgatg ccggtattca tgaatttacg aacctgaaat
    12121 ccatattgta ttcatgaaag taaataaaag aagacaggaa ttaatatctg aaatagtatc
    12181 caaaaggcaa cttgaccttt gtgtggtact cgaaaatgta caagatgacc acaatatagg
    12241 agcagtattg cgtacatgtg atgcagtcgg tatccaagaa ttgtatatcc tcaatactga
    12301 accactcata aaaaaacgat ttgtaaaagt tgggaaacga acctctgcag gtacccgaaa
    12361 atacctaaat gtgttttact acaatgaccc ggaggcatgt ttttcacaca tcaaaagcaa
    12421 atataaacgc attctcggaa caagccttgg agaaaaatcc gtttctctct acaaactaga
    12481 tctcacagaa tcagcagcat ttgtatttgg caatgaaaag accggaatca cacccggcac
    12541 acaaccttat atcgaacaaa attttctaat accgatgaat ggttttgcac aaagtctcaa
    12601 tatatcggta gcatgtgcta taagcttgta tgaatgctac agacaacgag aacagaaagg
    12661 aatgtacaca gatgcaatac aaaatgataa acaggagcta ctacaacact accttgaacg
    12721 atcgagagga aaagtaaacc cttctgtcga ttacaagatt ttttcgtccg atacggacaa
    12781 taagttggca ggaaataagt aattttgcag gctatgcgcc ttcagaaatt agagataaaa
    12841 ggttttaaga gttttgcaaa tgagacaacc gtacattttc atgaaaatgt aacgggagtg
    12901 gtaggcccaa atggtagcgg aaagtcgaat atcgttgatg cttttcgttg ggtgttgggt
    12961 gaacaaaaaa gtaaagacct ccgcttagat aaaatgtcaa gcgtaatatt caacggcact
    13021 tctaaaaaga aagaaagcgc actcgctcag gtttcactga catttgataa caacaaagga
    13081 atattgccct cagagtacaa cagcattacc atcacacgta tgctgtatag aaatggagac
    13141 tcagaatacc gaataaatga cgtcacttgc aggctaaaag acatcacaac tctattgctt
    13201 gacacaggca tgggttctga cagctatgcc atcattgcgc tcaatatggt ggacgattta
    13261 ctggcagata aagagaattc gcggcagaga atgttgtttc aggcttcagg tatcacaaag
    13321 tacaaggttc gcaaagaaga aacccttaga aagctaaaat caacagaaga cgacctcgac
    13381 cgggtgaaag acctgttgtt cgaaatagaa accaacatta aatcgctcga aaaacaagca
    13441 aaaagagctg agaaattttt cactataaaa gatgcttaca aagaccaaag ccttaagtta
    13501 gccatccatc aggtgtcaag gacaaaggca cagttcaaac aactttcaca acaaatagaa
    13561 aaagaagaag ataattacag agctgcagaa gtttcgtcca gaacacttga agccaaactc
    13621 gagcaaacaa aaaaatctaa ccttgacaaa gaaaaatttc tcacagaaaa gcaaaaggaa
    13681 accaacgaac ttttgtcaag aatacgctcc cttgaaaatg aaaaaa
//
LOCUS       DAOQQY010000041        13646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1505_length_13646_cov_6.965933, whole genome shotgun sequence.
ACCESSION   DAOQQY010000041 DAOQQY010000000
VERSION     DAOQQY010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13646
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_13646_cov_6.965933"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(592..2493)
                     /gene="rpsA"
                     /locus_tag="PLE23_03745"
     CDS             complement(592..2493)
                     /gene="rpsA"
                     /locus_tag="PLE23_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HMX82409.1"
                     /translation="MHELENLENQEVEQTSNAPVASEEVAEKPQTKAPVKKTAHDDFD
                     WSQGKRQTAHYSAEERKTLSEKYEAAMNTINNNEVVPAMVTAIHGGDVVLDINYKSDG
                     LLSLSEFRDMPDLKIGDYVDVYIESQEDTKGDLVLSRRKAKLLKAWQRIVDSYTNGTI
                     ITGVVVSKTKGGLIVDSEGLETFLPGSQIDIKPILDYDQYVGKTMEFKVVKINETIKN
                     AVVSHKALIENNIAGQRDQIISSLEKGQVLEGVVKNITDFGAFMDLGGVDGLLYITDI
                     SWGRINHPAEVLELNQKLNVVVLEFDEAKKRISLGLKQLQPEPWEVLNNEIQEGSVVK
                     GKIVNIEDYGAFLELQPGIEGLIHVSEISWSNQPINAREYFRLGQEFEAKVVTIDRSE
                     RKMSLSLKQLTEDPWDGIEARFPVGSKHTGEVKNLTPYGVFVELSDGIGGMVHISDLS
                     WTKRYAHPSEFTKVGAKIDIVILEIDKDSRKLALGHKQIEENPWDTFESVFPVGSYHE
                     ATILRKDDRGAIMQLPYGLEAFAPIKHIKKENGNMAEVEEVLTVKVIEFNRDDKRILV
                     SHTRYLEDIRKEADETVKKDKDDEEVKVRTTVNKQAEKIEKSTLGDLDVFSELKEQLD
                     DDAKKAKKD"
     gene            2770..4050
                     /gene="purD"
                     /locus_tag="PLE23_03750"
     CDS             2770..4050
                     /gene="purD"
                     /locus_tag="PLE23_03750"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMX82410.1"
                     /translation="MNILILGSGGREHALAHQIAKSPLCTKLFVAPGNGGTNEIAVNV
                     PLAINDFEKIADFALTTDVQLIIVGPEEPLVNGIVNYFKENEALQRIAILGPDKTGAQ
                     LEGSKAWAKSFMASEGIPTAAYREFTNKTRNEGIHYLDNCNLPIVLKADGLAAGKGVI
                     ITSDRDEAKENFNAMLDGMFGHAGNTIVIEQFLAGIEFSMFVITDGKDYILLPEAKDY
                     KRIGEGDTGPNTGGMGAVSPVPFLDDVLAEKVHTQIIKPTLNGLQKRNIDYRGFIFFG
                     LINVNGDPFVIEYNCRMGDPETEVVIPRIKSDLVPLFLGAANGHLPPELPEVTHETAA
                     TIMLVAQGYPGPYKKGNPITGTELVHESLVFHAGTLLNETLQTNGGRVMALTSFGMNI
                     QDAINKSLKAAELVHFEGKNYRKDIGKDLLIFEK"
     gene            4066..6528
                     /locus_tag="PLE23_03755"
     CDS             4066..6528
                     /locus_tag="PLE23_03755"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HMX82411.1"
                     /translation="MKFNILDISRAINGRFLGSHDEDVIEFLSTDSRNVIFEEQTLFI
                     ALPGSRKNGHDFIDELKTRGVRNFLVSDQRYVIAKDKYNYIVVPNTIVALQSLAAFHR
                     SRFSYPVMGITGSNGKTIVKEWLAQLLSKHFRIVRSPKSYNSQIGVPLSVWQMNTEHN
                     LAIFEAGISQKGEMQALEKIIKPDIGIFTYLGDAHSAGFYDAEEKLIEKLLLFEHAKV
                     LIYCADQLLPDTIIRQKFNGKLLAWSLEGNAPIQFIKTQGNSVITLTAIFPDTRFETN
                     IPFQDNASITNLCHAIVAAYSLGIMPEQIKDEVTQLASVAMRLELKEGINNCLLIDDT
                     YNADYDSLKIALQFLHQQSQKKIHTLILSDLLESEKTTTDMYRKVANLVNKYALDKII
                     TIGEKSSVLTNLLSDRIAYRHFKSTKAFIQHFHSSDFNSEAILIKGARHFQFEKIVHS
                     LSKNSHRTVLEINLSALIHNLNVYASLLRPGVKIMAMVKASAYGAGSDEVAKLLQYHK
                     VDYLCVAYPDEGVELRKTGVTLPVMVLNPDESTLEVTFKYNLEPEIYSPGILQSLIGQ
                     MSSGVTALGVHIKLDTGMNRLGFSESDIDYLIELLHQHQNIVVKSIFTHLAASEDPSQ
                     DEYTHKQATRFRQMAGKISAELNISPIMHILNSSGIGRFQEYQMDMVRLGIGLYGYDS
                     NGDVSRLLRSVHSFKASISQIKTVFANETIGYGRKGIVTKTSKIATISVGYADGFRRM
                     LGNGNYSVLINQTKVPTIGNVCMDMAMIDITDLKNVREGDEVIIFGEDLPVTEMAKTL
                     QTIPYEVFTGISPRVKRIYYQD"
     gene            6639..8609
                     /locus_tag="PLE23_03760"
     CDS             6639..8609
                     /locus_tag="PLE23_03760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMX82412.1"
                     /translation="MKSRFLIGLLVLLAGFAFGQKDQRVLFTVKNQPVTVDEFLYIYT
                     KNNGEKADFNKKSLQENLDLYKNFKLKVQYAKDQKLDTLPSIKTELAGYRKQLADSYL
                     MDKEVTDKLIKEAYERSKKDVHISHILKTLPENATPEDTLKAFNEISEIRAQLIKGAD
                     FNKIAVEKSDDKTAKDNKGDLGFFTALFPNGFYNVETAIYKMKPGEITAPIRTPLGYH
                     LFKLNEVRDARGEIEVAHILIRTPKDDPNNARSKMKIDSIYQLLQKGENYEDMAEQFS
                     EDKASSIKGGYIGFFGINHYEKPFEDAAFGLKKDGEYSSPIQTTAGWHIIKRISARPV
                     GDFETEKRRIEPKVKRDQRFTEAKEALLVKIKKQCGLKEDDKAMSAFIQSVDDKFFAN
                     DWKAPAQNRDKVLYSLDNGTKLTIGAFEDYLKDFTRKRMSMKQTHTVETAIRSMYNDE
                     LSTALFKYEEGKLEQKYPDFKSLMREYEEGTLLFAASEKMVWKKASEDSTGLKNYFDK
                     NLKGKYRWDRRVIADVVTIRIGGEPEAMKVAEYFQNHTREEVEQKFNTEKKNIVTEEK
                     TYEFGKNPIVDKMSWTKGEMSKVEKDEKTKAYYIIKIKEVLAPADKTLEESRGYAVAE
                     YGDYLEKAWVAELQKSIPAKVDTKVFESLVKK"
     gene            8686..9534
                     /locus_tag="PLE23_03765"
     CDS             8686..9534
                     /locus_tag="PLE23_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82413.1"
                     /translation="MLWATVSAAMLSCTSKKEQILAKVYDDYLYLSDVNGIFQNNMTP
                     EDSTKLLDLYVDDWVRNKLFENEALKRISNHDQIEKQVKEYRSSLINFAYQNLIVKER
                     LDTILDEESLSKFYEDNKEGFRLDNDVMRCFFVKVPKAKVNKVTFEKIWDKAKPGATE
                     ELLQICSKEATLYMLEDTVWNNADEIFLQIPKQSLKGKEYSDITKFRTEDDEYFYYLQ
                     IFKYLQKGETAPYSYARKRITDIFLLQRKKEIVEQAKDKILEKESRKHNFSIYTDRLK
                     IKSENK"
     gene            9607..10956
                     /locus_tag="PLE23_03770"
     CDS             9607..10956
                     /locus_tag="PLE23_03770"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMX82414.1"
                     /translation="MVKFIAFLLSFSSVIVLNGQNLLDKVVATVGGEPILLSEIEQQY
                     SYLKEGKAKVPESARCEILENMIVQNLLLNQAKLDSIVATDQEIDAEVNARIEEILRY
                     MGNDESQFEDYYGKNVSEVRNQMHDPIQQQILTRKMRQEIMSKISITPSEVKAYFNKI
                     PKDSLPYLSSEVELAEIVYKPKVNVAQKKVAFDKITDIRSRITQSGEDFAKLAEKYSQ
                     DPGSARNGGDLGLVKRGTFVPEFEAAAYNLDKDEVSPVIETEYGFHIIKMIERRGNNI
                     RCRHILIKPEITPEDLQKAKHELDSIRHLIVIDSISFSAAVKKFSNSKEYSYDNNGLL
                     SNPKTGTTYFEIGDLDSDVYFAQDKLKPGEISAPIEKKDQSGETSYVIIKLLSRSAPH
                     KANIEQDYKKIAEVALEHKRNASLVKWLKDHLDQTRIEIDKEYNSCGLQQKWETMPK"
     gene            11047..12909
                     /gene="htpG"
                     /locus_tag="PLE23_03775"
     CDS             11047..12909
                     /gene="htpG"
                     /locus_tag="PLE23_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HMX82415.1"
                     /translation="MSTHKGSISVQTENIFPIIKKFLYSDQEIFLRELISNAIDATTK
                     LKILASKGEMKGELGDLAIEVAVNKEAKTITITDKGIGMTQDEVEKYLNQVAFSSAQE
                     FIDKFKEDANIIGHFGLGFYSAFMVANKVEVNTLSYKEGAKAVQWSCEGEPEYSISSI
                     KKTTRGSEIILHVSPDAEEYLEDSKIQALLDKYCRFLPVEIKFGKKKTYVTDENDESK
                     TKEVEVDNIINNPNPLWAKQPADLTDEDYKSFYNELYPYSQPPLFWIHLNIDYPFNLT
                     GVLYFPKISSALEMQKNKIQLYSNQVYVTDDVKEIVPEFLTLLHGVIDSPDIPLNVSR
                     SYLQSDHNVKKITGYITKKVAEKLQQIFDKQRSEFEDKWNDIGTFVKYGMISEEKFYE
                     KAVHFCLLKNVDNAYFTIDAYKEKIKANQTDKNNKLVVLYTNSAIEQDVYIHSAKAKA
                     YDVLLLDQVLDNHFIQQLEYKNTDLKFVRVDADTVDKLIEKEQSFESVLNTEQIEMLT
                     EKFKSVLKDEHVAVTTAALSPDDQPATITRPEFMRRMKEMQAMQGMNFGNFPDSYNLV
                     INTNHPIYAEKLLILDSEQQTELTNYLYDLALLNQNMLKGPELTKFISKTIKMI"
     gene            complement(12956..>13646)
                     /locus_tag="PLE23_03780"
     CDS             complement(12956..>13646)
                     /locus_tag="PLE23_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82416.1"
                     /translation="TGSLTETKRFEIENHLLDCPLCSDAVDGIRGFQGVVANLPAESK
                     LNFSGKKQDIVPAKKTNWFRLAAVFTGIVVFTALLFQYFTDSNSQKIFAGAYKRLPPP
                     ESQTRSAGDSLTTDTVDEAMVLYGQNRFEEAISAFEIRLKDLPTDSQSYLYAGICYLE
                     TGKTNTAIDYFKKARINSEQFYPEATWYLALAYLKEGSNQQAVHLLEELAQTDNDFTQ
                     KAKELQADISR"
BASE COUNT         4604 a         2611 c         2594 g         3837 t
ORIGIN      
        1 aattgtcttt gtgttattca tacatctctg tataaataac tcgtttctta gggtctacat
       61 aaacgtttat ttccttccca acttttcaat gaactttgct atctattttg tactctattg
      121 tactattttt caaaagcgga cacaaagata cgtagttgat tttataacta caaacttttt
      181 taaaaatatt tttaaaaaaa aatttttacc ttctaattta cccccttttc gcctctcgtc
      241 tttactgaaa gcatttgttt aactgtttat cctttacttt gttcgatttt atctgaagaa
      301 tttttaaaat actttttaca tatcttgtag cttctgcttt cagaatgtgg gtgttttcac
      361 cttaatatac gtatatctta atcgagtagt tatgttgtcg gctgaatgaa ctgatagtta
      421 tttttgaaaa tacctgtcca aacaaattat cacaaacaaa attgtgatct ataataaagt
      481 tcacagatat ttggccctga taaattttta accttacttt aatcaataga aagtaaaaaa
      541 aatccctttt cggatggaaa agggatttta atattcaaaa gaaaaataga attaatcttt
      601 tttcgccttt ttggcatcgt catccagttg ttctttcaat tctgaaaaaa catccaaatc
      661 tcctaaagta gatttttcaa tcttctctgc ctgcttgttt acagtagtcc ttactttgac
      721 ttcttcatca tctttgtctt tcttcactgt ttcatctgct tccttcctga tgtcttcaag
      781 gtaccgcgtg tgcgaaacaa gaatacgctt atcgtctctg ttgaactcta taaccttgac
      841 agtaagtact tcttctactt ctgccatgtt tccattttct ttcttgatat gcttgattgg
      901 cgcaaatgct tcaagtccgt atggcagctg catgattgca cctctatcat cttttctgag
      961 aatggtagcc tcatgatatg aacctactgg gaatacactt tcaaatgtgt cccatggatt
     1021 ctcttcaatc tgcttgtgtc caagtgccaa ttttctgcta tccttgtcta tttcaagaat
     1081 gacgatatca atcttcgctc caacttttgt aaactcagat gggtgtgcat atctcttagt
     1141 ccagctcagg tctgagatgt gtaccatccc tcctattccg tcacttagtt ctacaaacac
     1201 tccataaggt gtaaggtttt tcacttctcc tgtgtgcttg ctgcctaccg ggaatcttgc
     1261 ttcgatacca tcccaagggt cttcagttaa ttgtttaagc gaaagtgaca ttttgcgttc
     1321 gctacggtca atagtaacca cttttgcttc aaactcctga cctaaacgga aatactctct
     1381 tgcattgata ggctggttgc tccatgaaat ttcggaaacg tgtatcaagc cttcaatacc
     1441 aggctgtaac tcaaggaaag ccccgtagtc ttctatattt acaattttac cttttacaac
     1501 tgatccttcc tggatttcgt tgttcaaaac ttcccatggt tctggctgta gttgtttcaa
     1561 accaagagaa attctcttct tggcttcgtc aaactctaga actactacat ttagcttttg
     1621 atttagctct agtacttcag caggatggtt aattctaccc cagcttatat ctgtaatata
     1681 aagcaaacca tctacaccgc caagatccat gaacgcacca aaatcagtga tgttcttaac
     1741 aacgccttca agtacctgac ctttttcaag acttgagatg atctgatctc tttgacctgc
     1801 gatattattc tcaataagag ccttatgtga tactacagca ttcttaatgg tttcgttgat
     1861 tttaacaact ttgaattcca ttgttttgcc aacatactgg tcgtagtcga gtattggctt
     1921 gatatctatc tgcgaacccg gaaggaatgt ttcaagacct tcgctatcta caataagacc
     1981 gcctttcgtt ttgctgacaa ccacacctgt aatgatggtg ccgttggtat atgagtcaac
     2041 gatgcgttgc catgctttaa gcagtttggc ttttcttctg cttagaacca ggtcaccttt
     2101 ggtatcctct tgtgattcaa tataaacatc aacataatca cctatcttaa ggtcaggcat
     2161 gtctctgaat tcagaaagtg acaacaggcc atcagatttg tagttgatat cgagtactac
     2221 atctccacca tgaatggctg taaccattgc cggaacaact tcattgttgt tgatggtatt
     2281 cattgccgct tcatactttt cagaaagcgt ttttctttcc tctgctgaat aatgagctgt
     2341 ctggcgtttt ccttgcgacc agtcgaaatc gtcgtgtgcg gtctttttaa caggtgcttt
     2401 tgtctgtggt ttttctgcaa cctcctcaga ggctaccgga gcatttgaag tctgctcaac
     2461 ttcttgattt tcaagattct ctagttcgtg catgaagtag aatcaggttt aaaaggttaa
     2521 gaaatatttt tagcggtgca aaggtacaac tttcttattg aaaataaaag ttttatgtat
     2581 atttttcatt aaaataattt cattaatgaa aggtgtatat aaatttgtag tgtgctaaat
     2641 gatttgatag actggcatac ttaattttgt gtttattcaa cattttatca aaggtcaata
     2701 ctgttcactt tagtagttaa gcaagctatc aaacagtttt ctgtatattt gcacaaaaaa
     2761 atttcacaaa tgaatattct gatactcgga agtggtggtc gtgaacatgc attggcacac
     2821 caaatagcca aaagcccact ttgtaccaag cttttcgttg cgcccggcaa cggtggcaca
     2881 aatgaaatag cagtgaatgt tccgctggca ataaatgatt ttgaaaaaat agctgatttt
     2941 gctctaacaa ccgatgtaca actcattata gtaggtcccg aagaaccttt ggtaaatggt
     3001 attgtcaatt atttcaaaga aaatgaagct ttacaaagga ttgccatcct aggtcctgat
     3061 aaaacaggtg cacaactcga aggctcgaaa gcatgggcaa aatcttttat ggcatctgaa
     3121 ggtatcccta ctgctgctta cagagaattc acaaacaaaa cccgtaacga aggaattcat
     3181 taccttgata actgtaacct gcctatagta ttgaaagcgg atggattagc tgccggtaaa
     3241 ggtgtcatta ttacgagtga ccgtgatgag gcaaaagaaa atttcaacgc tatgttggat
     3301 ggtatgtttg gccatgccgg aaacacaatt gtaatagaac aatttctcgc aggtattgag
     3361 ttttcgatgt ttgttattac tgatggaaaa gattatatat tattgccgga ggcaaaagat
     3421 tataagagaa ttggcgaagg cgataccggc ccaaacaccg gtggtatggg tgcagtatct
     3481 cctgtccctt tcctggatga tgtattggct gaaaaagtgc atacccagat catcaaacct
     3541 accttgaacg gcttacaaaa gagaaatatc gactaccgtg gatttatatt ctttggtctg
     3601 ataaatgtaa atggtgatcc gtttgtaatt gaatataact gcagaatggg tgaccctgag
     3661 acggaagtag tgataccacg cataaagagt gatttggtcc cattgttctt aggtgctgcc
     3721 aatggccacc tgccaccaga gctccctgaa gtaacccacg aaacagcagc aacgattatg
     3781 ttagtggcac aaggatatcc aggaccatac aaaaaaggaa atccgataac aggtactgag
     3841 cttgtgcatg aaagtttggt gttccatgcc ggtactttac tcaatgaaac tttacaaact
     3901 aatggcggcc gtgttatggc acttacctca tttggtatga atattcagga tgctataaat
     3961 aagtcgctta aagcagctga acttgtacat tttgaaggaa agaattacag aaaggatatt
     4021 ggtaaagatt tattgatttt cgaaaaataa taagcggtct aagtaatgaa gtttaacatt
     4081 cttgacatca gcagagcaat caatgggcgg tttttaggca gccatgatga ggatgttata
     4141 gaatttttgt caaccgacag ccgcaatgta atattcgaag aacaaacatt atttattgca
     4201 ttgccgggct ccaggaaaaa cgggcatgat ttcattgatg aattaaagac tcgaggtgtt
     4261 cgaaattttt tggtgtcaga ccaaagatat gttattgcta aggacaagta taattatatt
     4321 gtagttccaa acacgatcgt agcattacaa tcacttgcag catttcaccg ttcacgattt
     4381 agttatccgg ttatgggtat tacaggtagc aacggcaaaa ctattgtaaa agaatggctg
     4441 gctcagctgc tgagcaaaca tttcagaata gtccgcagcc caaagagcta caacagccag
     4501 ataggtgtgc cactctcagt atggcaaatg aacactgaac acaacctcgc tatttttgaa
     4561 gcaggtatct ctcagaaagg tgaaatgcag gctcttgaaa aaataataaa acctgatatc
     4621 ggtattttta cttatcttgg ggatgcccac agtgcagggt tctatgatgc tgaagaaaaa
     4681 ctaatcgaaa aacttctttt attcgaacac gctaaagtct tgatttattg tgctgaccag
     4741 ctactacctg atacgataat ccgacagaaa tttaacggca aattacttgc ttggtcactt
     4801 gaaggaaatg cacccattca atttataaaa acacaaggaa attctgtcat cacacttact
     4861 gcaatttttc ctgataccag gttcgagacc aatatcccgt ttcaagataa tgcttctatt
     4921 actaaccttt gtcatgctat agtagctgca tattccttag gtatcatgcc cgaacaaatc
     4981 aaggatgaag taacccagct ggcttctgtg gccatgagac ttgaattgaa agaagggatt
     5041 aataattgtc tgctgataga tgatacatac aatgcagact atgattcact caagatagcc
     5101 ttacagtttt tgcaccagca aagtcaaaaa aaaattcata cgctgatact ttccgatctg
     5161 ttggagtctg aaaaaactac cacagatatg tacaggaagg tggccaacct ggtaaataaa
     5221 tatgcgttag acaaaatcat caccattggt gaaaaatctt cggtcctcac caatctactc
     5281 tccgaccgca ttgcttacag acattttaaa tctacaaaag cttttataca acatttccat
     5341 agctcggact ttaactctga agccatttta atcaaaggtg ccaggcattt tcaatttgag
     5401 aaaattgtac acagcttgtc aaaaaattca cacagaactg tacttgaaat aaatttatca
     5461 gcacttattc acaatctgaa tgtctatgct tctttgctaa gaccaggcgt gaagataatg
     5521 gccatggtga aggcctcggc atacggtgca ggcagtgatg aggtagccaa actgctgcaa
     5581 tatcataagg ttgactacct ttgtgtcgct tacccggacg aaggtgtaga gctgcgcaaa
     5641 actggtgtta ctttaccggt aatggtgctc aaccctgatg aatcaacact tgaggtcacc
     5701 tttaaataca atcttgaacc tgaaatatac agcccgggta ttctgcaatc attaataggg
     5761 caaatgtcat ccggagttac cgccctgggt gtacatatca agttagatac aggaatgaat
     5821 aggctcggat tttcggagtc agatattgat tatctgattg aattattgca ccagcatcag
     5881 aatattgtcg tgaaaagcat ttttacacat ctggcagcaa gtgaagaccc gtcacaggac
     5941 gagtacacgc ataagcaggc aacgagattc agacaaatgg ccggcaaaat atcagcagaa
     6001 ctcaatattt cgcccatcat gcacatactg aattcttctg gtattggtcg gtttcaggaa
     6061 tatcaaatgg atatggtaag gctgggtata ggcctgtatg gttatgattc gaatggagat
     6121 gtgagcagat tattgcgcag tgtgcattca ttcaaagcta gtatttcgca gataaaaacg
     6181 gtattcgcca acgaaacaat aggatacgga cgcaaaggta ttgtcacaaa gacatcaaaa
     6241 attgccacca taagtgtggg gtatgcagac ggattcaggc gtatgcttgg taatgggaat
     6301 tacagtgtac tcatcaacca aacaaaggtg cccaccatcg ggaatgtgtg tatggatatg
     6361 gcaatgatag acatcacaga tctgaaaaat gtacgagaag gcgatgaagt aatcattttt
     6421 ggtgaagacc tgccggtaac agagatggca aaaacattac agacaatacc ttatgaagta
     6481 tttaccggaa tttcaccacg agtcaagcgc atatattatc aggattgaga tgtaaaaagc
     6541 atcaaagaat cttaaatatt tctaaaggtt atgagtattt ataactttcg aataaaataa
     6601 ttttaatttt gcccaaataa attaataaac catctaacat gaaatcaaga tttctgattg
     6661 gcctgttggt tctgctagca ggttttgcat ttggccagaa agaccaaaga gtattgttta
     6721 cagtgaaaaa ccaacctgta acagttgatg aatttcttta tatttataca aaaaacaatg
     6781 gtgaaaaagc cgattttaac aaaaaatcac tccaggaaaa tctagacttg tataaaaatt
     6841 tcaaactgaa ggtacaatac gcaaaggacc aaaagcttga taccctacct tctataaaaa
     6901 cagaattagc cgggtacaga aaacaacttg ccgactcata cttgatggat aaagaagtga
     6961 cagacaaact catcaaagaa gcttacgaac ggtcgaaaaa agatgttcat ataagccaca
     7021 ttctaaaaac actgcccgaa aacgccacac cggaagacac gctgaaggct ttcaacgaga
     7081 tatcagaaat aagagcacaa ttgataaaag gagctgactt caacaaaata gctgttgaaa
     7141 aaagtgatga taaaaccgcc aaagacaata aaggcgatct tggttttttc acggccttgt
     7201 ttcctaacgg attctataat gtagagacag ctatatacaa gatgaaaccc ggagaaataa
     7261 ctgcacctat acgcaccccg cttggatatc acctatttaa gctcaatgaa gtacgggacg
     7321 cccgaggcga aatagaagta gcacatatac tcataagaac acctaaagac gacccgaata
     7381 acgctcgaag taaaatgaaa atcgactcga tataccagtt gttgcaaaag ggtgaaaact
     7441 atgaagatat ggccgaacaa ttctctgaag ataaagcatc atccatcaaa ggtggctata
     7501 tcggattttt tggaattaac cactatgaaa aaccttttga agatgccgct tttggtctga
     7561 aaaaagacgg cgaatacagt tcaccaatac agactactgc aggctggcat atcatcaaac
     7621 gaataagtgc aagacctgtt ggagattttg aaaccgagaa aagacggata gagccaaagg
     7681 tgaagcgcga tcaaagattt acagaagcaa aggaagcatt actggtaaaa atcaaaaaac
     7741 aatgtggtct taaggaagat gataaagcaa tgagtgcctt cattcagtct gtagatgata
     7801 aattctttgc caatgactgg aaggctccgg cacaaaatcg ggacaaagta ttgtactcac
     7861 tcgataatgg caccaagcta accatcggag catttgaaga ctacctcaag gattttacac
     7921 gcaagaggat gagtatgaaa caaacccata ccgtcgaaac cgcaatccgt tctatgtata
     7981 acgatgagct aagcacagca ttatttaaat acgaagaagg caaactagaa caaaaatatc
     8041 cggattttaa atcgctgatg cgtgaatacg aagaaggtac gctcttgttt gcggcatcag
     8101 aaaaaatggt ttggaaaaaa gcatctgaag attctactgg tttaaaaaat tatttcgaca
     8161 aaaacctgaa aggaaaatac cgctgggata gaagagtgat tgcagatgtc gtaacaataa
     8221 gaataggtgg cgaacccgaa gccatgaagg tagccgaata tttccaaaat catacaagag
     8281 aagaggttga acaaaaattt aacaccgaaa aaaagaatat cgtcacagaa gaaaaaacct
     8341 acgagtttgg taagaatccg atagtcgata aaatgtcgtg gaccaaaggc gaaatgtcaa
     8401 aagtagaaaa agacgaaaaa accaaggctt actatattat aaaaattaaa gaagtgttgg
     8461 cacctgctga taaaacactt gaagaatcgc gtggatatgc agtagcagaa tatggtgatt
     8521 accttgaaaa ggcctgggta gctgagcttc aaaaatccat tcctgctaaa gtagatacca
     8581 aagtatttga atcactcgta aaaaaatgat aaagatatat cggtatcttt gtctatgata
     8641 aggagtattc tacattattg caattggcaa aagcctgtat ttattatgtt gtgggcgacc
     8701 gtatcggccg caatgcttag ctgtacttca aaaaaggaac aaatattggc taaggtatat
     8761 gatgattatc tgtacctatc agatgtcaat ggcatttttc aaaataacat gactcccgaa
     8821 gacagtacta aacttcttga tctgtatgtc gatgactggg taaggaacaa attatttgag
     8881 aacgaagcac tcaaaaggat ttctaatcat gaccaaatag aaaaacaggt gaaagaatat
     8941 cgttcatcgc tcatcaattt tgcataccaa aatcttattg taaaagaaag actcgatact
     9001 atacttgacg aggaatcact ttcaaaattt tatgaagaca ataaagaagg attccgactt
     9061 gataatgatg tgatgcgatg cttttttgtt aaagtcccaa aagcaaaagt caataaggta
     9121 acttttgaaa aaatatggga taaagcaaag cccggagcta cagaagaatt gctacaaata
     9181 tgttcaaaag aagctaccct gtatatgctc gaagacacag tgtggaacaa tgccgatgag
     9241 atattccttc agatacctaa gcaaagccta aaaggcaaag aatattccga tatcaccaag
     9301 tttaggactg aagatgatga atatttttat tacctacaga tattcaaata cctgcaaaaa
     9361 ggagaaacag caccatacag ttatgcacgt aaaaggataa cagatatatt tttgctacaa
     9421 cgaaaaaaag aaatcgtaga gcaagcaaag gataaaatac ttgaaaaaga atccagaaaa
     9481 cacaatttct ccatttatac cgaccgcttg aaaattaaat cagaaaataa atagtaattt
     9541 cgcgtctcat atccggattt agggtatcaa caacgttata ataaaatact cagcacaaca
     9601 attaatatgg ttaagtttat tgcattccta ttatcattct cctctgttat tgtactaaat
     9661 ggtcagaacc tattagacaa agtagtagca actgtaggcg gagaaccaat tttactatcc
     9721 gaaatcgaac aacaatattc ttacctcaaa gaaggaaaag ccaaagtacc ggaatctgcc
     9781 agatgtgaga ttcttgaaaa tatgattgta caaaatctct tgctcaatca ggcaaaactg
     9841 gatagtatcg ttgctacaga tcaggaaata gatgctgaag taaatgccag aatcgaagag
     9901 atactgcgct acatgggtaa tgacgaaagc cagtttgaag attattatgg caaaaatgtg
     9961 tcggaagtgc gcaatcagat gcacgaccct atccaacaac aaatacttac cagaaaaatg
    10021 cgtcaggaga taatgtctaa aataagcatt acgccatctg aggtcaaagc ttatttcaac
    10081 aaaatcccta aagactcact accgtatctc agttcagaag ttgagttggc agaaatagta
    10141 tataagccta aagtaaatgt tgcacagaag aaggtagcat ttgataaaat cacagatatt
    10201 agaagccgca taacacagtc tggcgaagat tttgcaaaat tggctgaaaa atattctcaa
    10261 gatccgggtt cggcacgtaa cggaggcgac cttggtcttg taaaacgagg tacgtttgta
    10321 ccagagtttg aagctgcggc ctacaacctt gataaagatg aagtctcacc cgtcatagaa
    10381 acagaatatg ggttccatat tattaaaatg atagaaaggc gtggtaataa cattagatgc
    10441 agacacattc tcatcaaacc tgaaataaca cctgaagacc tccagaaagc aaaacatgaa
    10501 ctggatagca tccgccatct gatagtaatt gattcaataa gtttctcagc agcggtaaaa
    10561 aagttttcaa atagtaagga atacagttat gacaacaacg gactcctttc caatccaaaa
    10621 acaggaacca cctatttcga aatcggtgat ctggattcgg atgtatattt tgcacaagat
    10681 aaactcaaac cgggagaaat atcggctcca attgaaaaga aagaccaaag cggtgagacg
    10741 agttatgtca tcatcaagct attaagccgg tctgcaccac ataaagccaa catcgagcaa
    10801 gattataaga aaatagcaga agtagcattg gaacacaaaa ggaatgcctc acttgtcaaa
    10861 tggcttaaag accacctcga tcaaactaga atagaaattg acaaagaata taattcctgt
    10921 ggtctgcaac agaagtggga aaccatgcct aagtaaaaat tacgagcaat aacattaaaa
    10981 ccgacaaatt gtcagcctta ttgcaggtgg catgcagttt gacctgttgc tcataaagta
    11041 ataattatga gtacacataa aggttccatt tctgttcaga cagaaaatat tttcccgatt
    11101 attaaaaaat ttctttactc agaccaggaa atcttcctgc gtgagctgat ttcaaatgcc
    11161 atagatgcca ccaccaagct caagatactt gcatccaaag gtgaaatgaa aggcgaactt
    11221 ggcgatctgg ccatagaagt agctgtaaac aaagaagcaa aaaccatcac cattactgac
    11281 aaaggtattg gaatgactca ggatgaagta gaaaaatacc tcaaccaggt agctttctcc
    11341 agcgcccagg aattcatcga caaattcaaa gaagatgcta acattattgg ccactttggt
    11401 cttgggttct actcagcatt tatggtagct aataaggtag aagtcaatac tctttcttac
    11461 aaagaaggag caaaagccgt acaatggagc tgtgaaggcg aacccgaata ttctattagt
    11521 agcataaaga agactacacg cggttcagag attattctgc atgtatcacc cgatgctgag
    11581 gagtatctgg aagattccaa aatacaggct ttgcttgata aatattgtag atttttaccg
    11641 gtagaaataa aatttggaaa gaaaaagacc tacgtcactg acgaaaatga cgagtcgaaa
    11701 accaaagaag tagaagttga caacatcatt aacaacccaa atcctctttg ggccaaacaa
    11761 cctgcagatc tgacagatga agactataag tcattttaca acgaacttta cccgtacagt
    11821 cagccaccac ttttctggat tcatctcaat atagattatc ctttcaatct cacaggcgta
    11881 ttgtatttcc caaagataag cagtgccttg gagatgcaga aaaacaaaat tcagctgtac
    11941 agcaatcagg tctatgtaac ggatgatgta aaagaaatcg tgccagagtt cttgacattg
    12001 ttacatggtg ttattgattc acctgatatt ccgctcaacg tatcaagaag ttaccttcaa
    12061 agtgaccaca acgtaaagaa gattaccggc tatattacta aaaaagttgc tgaaaaactc
    12121 caacaaatat tcgacaaaca aaggtcagaa tttgaagaca aatggaatga catcggtaca
    12181 tttgtcaaat acggtatgat ttctgaggaa aaattttatg aaaaagctgt ccatttctgt
    12241 ttgttgaaaa atgtagataa tgcatatttc accattgatg cttacaaaga aaaaataaag
    12301 gcaaaccaga ctgacaaaaa taataaactt gtggtactct ataccaatag tgccatagaa
    12361 caagatgtgt acatccattc tgcaaaagca aaagcctatg atgttctgtt gcttgaccaa
    12421 gttcttgaca accatttcat ccagcagctt gaatacaaaa atactgattt gaaattcgta
    12481 cgagtagatg cagacaccgt tgacaaacta atcgaaaaag aacaaagctt cgaatcagtt
    12541 ctaaacacag agcaaataga aatgctaact gaaaaattca agtccgtatt aaaagatgaa
    12601 catgtagcag tgaccacagc agccctgagt cctgacgacc aacctgccac catcaccaga
    12661 ccagaattta tgcgcagaat gaaggaaatg caagccatgc aaggcatgaa tttcggaaat
    12721 ttcccagaca gttacaattt ggtcatcaac accaaccacc caatatacgc tgaaaaacta
    12781 ttaatactcg atagcgaaca acaaacagaa ctgaccaact acttgtacga tctggctttg
    12841 cttaaccaaa atatgttaaa aggacccgaa ttaaccaaat ttatttcgaa aacaataaaa
    12901 atgatctaac agaaccttta aataatttaa aaaattgatt gatagtcaga aagagttacc
    12961 ggctgatgtc agcttgtaac tcttttgctt tctgtgtaaa gtcattatct gtctgtgcga
    13021 gttcttctag caaatgaaca gcttgttggt tagatccttc ttttaagtaa gcaagagcca
    13081 gataccaggt agcttcagga tagaattgtt cagaattaat gcgtgctttt ttgaaataat
    13141 caatagcagt atttgttttt ccggtctcga gatagcatat acctgcatat aaataggact
    13201 ggctgtcggt tggtaaatct ttaagcctta tttcaaaagc tgaaattgct tcttcaaatc
    13261 tgttttgtcc gtataatacc attgcctcgt caactgtatc tgtggtcagc gagtcacctg
    13321 cagaacgagt ctgactctca ggtggtggca ggcgtttgta ggctccggca aatatttttt
    13381 ggctattaga atctgtaaaa tactgaaaca acaaagctgt gaacaccaca atgcccgtaa
    13441 aaaccgcagc cagtcgaaac cagttggttt tctttgctgg aacaatatct tgttttttac
    13501 cagagaagtt aagcttagat tcggctggca gattagccac aacaccctga aagcccctaa
    13561 taccatcaac tgcatcagag caaagtggac aatccagaag gtgattttca atttcgaatc
    13621 gttttgtttc tgtcaaacta cctgtt
//
LOCUS       DAOQQY010000042        13594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1513_length_13594_cov_6.831450, whole genome shotgun sequence.
ACCESSION   DAOQQY010000042 DAOQQY010000000
VERSION     DAOQQY010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13594
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1513_length_13594_cov_6.831450"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..3944
                     /locus_tag="PLE23_03785"
     CDS             18..3944
                     /locus_tag="PLE23_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82417.1"
                     /translation="MKKLCIGLLSLVMLLTAGEIAARENPNQGLGTVVYNKKPSKRGE
                     CKPSTSSTDLNINNVRARLLGGGDMWWDLKDPKYIVPNVEPGKGIPEVSSLFSGAVWL
                     GGFDDGGNLKMAAQTYRSSGNDFWPGPLTSDGLVEESTCAKWDRHFTVYGKNIKSHID
                     NFKKSIDPVTGENKYDPNTIPADVKGWPSRGNPYFEEINGFSLPDDDNGLAGFHDAGT
                     PDFEYDPKDGDYPVIEIRGCELTNYPDQMVFWIYNDAGGVHTQTNGDAIKMEVQVQAF
                     AYASNDELNDMTFYRFKLINRAIEPIDSTFFAMWTDPDLGCATDDYIGCDTSRSLMYI
                     YNQDAIDGDPGTNCGGTPTYNDKIPMLGIDYFRGPNDENGNEIGMTSFIYYNNGGIGN
                     NPPGTEDPNGPIEHYRYLSGSWRDGKRITKGGSGYNTLGTPTKYTFPEAPNDPNGWSM
                     CTANLGIGDRRTLQASGPFRLDPGKKNELIIGVVWVPNIPHPCPNPAKLTNADDLAQA
                     LFNNCFKVTDGPDAPDVDFIELNNQLVMVLTNDSLTSNNAFENYSGVDLRAPEGTVDS
                     LYRFEGYKIYQVAGSDVTNLELNDPEKAQLIAQYDLKNGVTQIFNWNTIDKPNSNTNE
                     KYWIPTLKVVGQDLGIKHTLSITEDVFAKGDRKLINHKQYYFKVVAYAYNNYEKFDPI
                     TGLGQRTPYLEGRRVFTAVGIPRPIIDVNQNAEYGTLGLVSRVDGNGTGGFDLEISDD
                     TRSLLFEQSYSGDVPYLPGRGPIDVKIYNPVDTKKGEFILTFVDSDMSNNKLDVGCGW
                     TLKKVDDPSFEVLSEESIDQLNEQVIAKYGFSITIGQIPDAGTTFDAKNGFVGTRIKY
                     AEGAGQKWLVNIPNSPGVGPLHYIKTNVLKTQWNALDPKGAYINDLNIYPYILCDYVD
                     STMGFISPAWQDLTNKTAIAPMKLSELNNVDIVFTKDKSKWSRCVVIETTPKKLGEIN
                     TPIGNAKQFDLRKSPSVGKDDNNGDGLPDPDGDGDGMGWFPGYAVDVETGQRLNIFFG
                     EASAYAKGAPFDDNGTFTGGDMMFNPSNHTVLNNAAGNIYDAIFGGLHTVYVTKSKYD
                     GCDSIRTLLKEPTTGLKKVQVIRKLTWTFMPFFAPETQTTGISNGIIPNDCVVSARVD
                     NIYHVANQRVTDQYKGYPSYKITILGANETTQLTDSQRVESLDNINIVPNPYYAYSAY
                     ETSQFTNTVKITNLPNKCTVSIYSLDGKFIRQYKRDEVPGSAANRPNAPFSNYQVIPD
                     LNWDMRNDKGVPIGSGVYLIHIDAPGVGERTLKWFGVGRQFDPSGL"
     gene            4036..5076
                     /locus_tag="PLE23_03790"
     CDS             4036..5076
                     /locus_tag="PLE23_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HMX82418.1"
                     /translation="MKKLLYTILTVFTFSSVFAGNPDRQGESGAAQLLLNPWARSAGL
                     HTMTTANVIGVEAMQLNIAGLSRVNKTQVMIGNTQYLKGTGIGLNAIGLSQKLGENSA
                     IGIQLMAVDFGKIPITTTNQPEGIGATYSPSFFNIGLGYSYTFQNKISVGFLVRGVSE
                     TLADISAFGIAMDAGVQYVTGKKDEFKLGISLRNIGSPMKFGGEGLSIAQNAPEGNYQ
                     LTFNVRPANYELPSMLNLGVSYDFDLGENLILTGIGNFTSNSFAQDQVGLGFNFAIGK
                     FFQVRAGYKYEFGQFNESGLYASMDKGLSAGVSVEIPLKKNSESRLGLDYGYRHTTRW
                     SGSHSIGVSINL"
     gene            5174..5647
                     /gene="greA"
                     /locus_tag="PLE23_03795"
     CDS             5174..5647
                     /gene="greA"
                     /locus_tag="PLE23_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMX82419.1"
                     /translation="MTEYSYLTQEGYDKIKAELEDLKSNGRPEIARAIAEARDKGDLS
                     ENAEYDAAKNAQGMLELKINELEKLLGNVRIIDESTVDLSKVSLLCNVTIRNIKTKAE
                     ITYKLVSESEADLKLKKISIKSPIGNGLLGKKVGDIAQVQTPNGVIEFEIIDISL"
     gene            5651..6049
                     /locus_tag="PLE23_03800"
     CDS             5651..6049
                     /locus_tag="PLE23_03800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HMX82420.1"
                     /translation="MSTIFSKIISGEIPCFKVAEDEQHLAFLDINPVAFGHTLVIPKR
                     EIDYYFDLDDEEIARLMIFSKKVALALKSVVPCTKIGISVIGLEVPHTHVHLVPINAI
                     ADMTFGKKIEISIDELTKLASQLADKIKIS"
     gene            complement(6036..6605)
                     /gene="ruvC"
                     /locus_tag="PLE23_03805"
     CDS             complement(6036..6605)
                     /gene="ruvC"
                     /locus_tag="PLE23_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMX82421.1"
                     /translation="MNKDISYKILGVDPGTNFLGFAIIETICDPPKVMSLDVINLSKY
                     KDQQTKLLHIHREISKLIEIFHPKQLAIEEPFYANNVQSMLKLGRAQGVAIAAAINMG
                     LTIAEYSPKKVKQSITGNGNAAKEQVARMLPHLVDMNNQVYEKLDATDALAVAVCHYS
                     QNKLLTTSGKKYSGWQEFVKNNETRLKKS"
     gene            6899..7678
                     /locus_tag="PLE23_03810"
     CDS             6899..7678
                     /locus_tag="PLE23_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82422.1"
                     /translation="MSHLDQVNGRVVLIKSLLSGIFLSAITPVKSGDYGGRLVFIRKD
                     DWAIALNAVFWGNGCQLLWLMVCGLLGLLYLSTVLSENLLQAKTSLVVVLIVVMLMLI
                     IVWFYLRIALVRKLVFYLPFKEKLRKVYYNFLKIKNRNDLYKTLIIAGLRYFTYIIQS
                     MLLISVFNNTIAFSLLLSGVMTVFFIQAILPVLSFLGLIGRTGIAIFVLNKIGVSEMD
                     SGVSSIMLWVVNIFIPSVAGLYFLFRKMINKQNEKKELDIS"
     gene            7650..8483
                     /locus_tag="PLE23_03815"
     CDS             7650..8483
                     /locus_tag="PLE23_03815"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HMX82423.1"
                     /translation="MKKRNWILVSTMMVLLVTAGFRPTVKPDELYDPCAINNWTFTGG
                     EEITYKIYYNWNFMWLAAGEAVFRVYDEGDTYHLSVKGTTYKSYEWFFKVRDSYDTWV
                     DKTTLLPKVAIRDVSEGNYKLYEKMIFDQENSKITSYRGNTPKDAVKKEFRINDCMHD
                     LISIIYHARNLEYEKFAKGQEYPIKIFMDKEVWPLSVKYKGRYKQVTVKGGTKYKAFK
                     FSPDVISGEVFKEGTQMDIWVSDDNNRIPVYIESPVSVGSVKIVLKDYKGLKYPLTAK
                     I"
     gene            8480..9097
                     /locus_tag="PLE23_03820"
     CDS             8480..9097
                     /locus_tag="PLE23_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HMX82424.1"
                     /translation="MRYQAVGNSMLKKILYIAVGLIIVFAIVKVNDIYHSFTARKTAE
                     ESVVLLESIRKVTKLVAVEGYFSEVYDYKDYYGFDISPLRKKALIRVKAKVSVEYDFD
                     KLKITADDSRKIIHISSIGKPQILSVDHKLDYYDISQGTFNSFTSEDYNRMNQNAKDF
                     IISKVKESDLMNQAGEQMDGIIETIKTLTESRGWQIVVEKEPTIQ"
     gene            9152..10159
                     /gene="nadA"
                     /locus_tag="PLE23_03825"
     CDS             9152..10159
                     /gene="nadA"
                     /locus_tag="PLE23_03825"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMX82425.1"
                     /translation="MSYNYQEAVGELKRRGYLAVDVDPTLDFEAEIIRLKKEKNAVIL
                     AHYYQDSEIQDLADFVGDSLGLSQEAAKTKADMIVFAGVHFMAETAKMLSPQKKVVLP
                     DLNAGCSLADSCPAPLFSKFKEKYPEHIVVSYINCSAELKTLTDICCTSTNAVHIIES
                     IPKDKQIIFAPDRNLGKYLIKKTGRDMVLWEGACMVHEIFSKEKIVALMHTHPEAKLI
                     AHPECEPHILEHAHYIGSTSGLLKFSKTDPAKEFIVATEPGIIYNMERASPDKVFIPA
                     PPDNHCACNECPHMKRNTLEKLYLCMKYELPEILLPDWVIDQGKKSIERMLDISAKAG
                     L"
     gene            10222..10908
                     /locus_tag="PLE23_03830"
     CDS             10222..10908
                     /locus_tag="PLE23_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HMX82426.1"
                     /translation="MMMLYVISAVFGVVGMLVSARLKSKFSHYALVPVTNGMTGRDVA
                     EAMLKYYNIHDVQVTAVEGTLTDHYNPQDKTVNLSQDVYYGNHVSAAAVAAHECGHAV
                     QHATAYTALTMRSRLVPIVSLSANLQQYLFMFGVVGMASFNSPLLLIIAIAAFGVTAL
                     FSLITLPVEFDASSRALVWLNHTNVVSGAEYDGAKDALRWAALTYVVAALAAVAQLVY
                     LVMMFMSRRD"
     gene            11015..11581
                     /gene="frr"
                     /locus_tag="PLE23_03835"
     CDS             11015..11581
                     /gene="frr"
                     /locus_tag="PLE23_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMX82427.1"
                     /translation="MTEQEIQIITQKAKNSMDHSLDHLQKELSKLRTGKASTTMLEGI
                     LVDYYGSPTLLNQVANVATSDARTISIQPWEKKMLSHIEKAIFEANLGVTPQNDGEVI
                     RIIIPPLTEDRRKDLVKQAKHLGEESKVGLRNARRDAMEVFKKAIKDGLSEDIGKRKE
                     EETQKMLNSYVEKIDTIVSAKEKEIMTV"
     gene            complement(11652..12461)
                     /locus_tag="PLE23_03840"
     CDS             complement(11652..12461)
                     /locus_tag="PLE23_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82428.1"
                     /translation="MRKLFAFVLCMFLAFITTISAQDSSLIVTKLNIPNTELVLLDRF
                     RNIYVATNEPRLLQYDSKGTKMAEYTEVRYGSFTVIDINNPLSILVYYADFGIVQILD
                     RNLTLQSEFNLRTFGYLTNTAIGLGADNNIWFYNMETFRLIKIDSFGKIIRKSNELTG
                     LVGKSAFTGQIWEQDNFLLMRAPDFGWMLFDDFGSYIQTLPLPDEEIQYFTNSRIYYR
                     QEDYIDVLDLNNMRVKSYPIPTFLDQANNIQYIYKCWMEITADTIRIFTLD"
     gene            complement(12458..13228)
                     /locus_tag="PLE23_03845"
     CDS             complement(12458..13228)
                     /locus_tag="PLE23_03845"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HMX82429.1"
                     /translation="MQKIYLATDFHLGVDAGSTSLEREKSIVRWLDMAAADASDIFLV
                     GDLFDFWFEYKYTVPKGFVRLLGKLASLRDRGINIEVFTGNHDMWMFGYLTHELGIPV
                     HKQPIMKTFFGKKYFIGHGDGLGPGDHGYKFIKKVFANPICQWLFARLHPNFSFGLAN
                     YWSAKSRNANTDPDHFLGKDREWLVQFCKETLATQHYDAFIFGHRHLPIDFYFTKENS
                     RYVNLGDWIRYNTFAVINADGIELHSWEKDTKIFCNRS"
     gene            complement(13242..>13594)
                     /gene="nbaC"
                     /locus_tag="PLE23_03850"
     CDS             complement(13242..>13594)
                     /gene="nbaC"
                     /locus_tag="PLE23_03850"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HMX82430.1"
                     /translation="ELFYQLEGDIQVKIIHPDGKPETIDIREGDMFLLPPHTPHSPQR
                     PANTIGLVIERTRKPKEIDKLMWFCEKCGNKLHEASFEMQDIVNQLPPAMNHFMNSED
                     LRTCNSCGSVMEKV"
BASE COUNT         4207 a         2358 c         3030 g         3999 t
ORIGIN      
        1 atttaaattt taaagacatg aaaaagcttt gtattggttt gctcagcctt gtaatgttgc
       61 tcacagctgg tgaaattgca gcaagagaaa atcccaatca aggtttagga acagtggttt
      121 acaataagaa gccttcaaaa aggggcgaat gtaaaccatc cacttcctct actgacctaa
      181 atatcaataa cgttcgtgcc cgtttactgg gtggtggcga tatgtggtgg gacctcaaag
      241 atccaaagta catagtccct aatgtggagc ctggtaaagg tatccctgag gtgtcatcgt
      301 tattttcggg tgcagtatgg ttgggtggat ttgacgatgg aggtaacctt aaaatggcgg
      361 ctcaaacata ccgttcatca ggtaatgatt tttggcctgg gccactcact agtgatggtc
      421 ttgtcgaaga gtcaacctgt gctaaatggg acagacattt tacggtttat ggcaaaaata
      481 tcaaatctca tattgataat ttcaagaaga gtattgaccc cgtgaccggt gaaaataagt
      541 atgatcctaa taccatacct gcagatgtta aaggttggcc ttcaagagga aatccatact
      601 ttgaggaaat caatggattc agccttccgg atgatgataa tggtcttgca ggtttccatg
      661 acgccggtac tccggatttt gagtatgacc ctaaggacgg agactatcct gttattgaga
      721 taagaggttg cgagttaacc aattatcctg accagatggt tttttggata tacaatgatg
      781 ccggtggtgt gcacacacag actaacgggg atgcaatcaa aatggaagta caggttcagg
      841 cattcgctta tgcttcgaat gacgaactga acgacatgac tttttacagg tttaagctta
      901 ttaatcgtgc tattgagcct attgacagta cgttttttgc catgtggaca gacccggacc
      961 ttggttgtgc gacagatgac tacataggtt gtgatacatc acgcagcttg atgtacatct
     1021 acaatcagga tgccatcgac ggtgaccctg ggacaaactg tggcggtact cctacataca
     1081 atgataaaat accaatgcta ggtatagact atttccgtgg accaaacgat gaaaacggaa
     1141 acgaaattgg tatgacatca ttcatttatt acaacaatgg aggtattggc aataatcctc
     1201 cgggtacaga agaccctaat ggtccaatcg aacattacag gtatttgtcg ggctcttgga
     1261 gagacggtaa aagaattaca aaaggaggct caggttataa tactttagga acgcctacaa
     1321 aatacacatt cccggaggct ccaaatgatc cgaatggatg gagtatgtgt acagccaatc
     1381 tgggtattgg tgacagaaga acacttcagg catcagggcc tttcagactt gacccgggta
     1441 aaaagaatga gttgattata ggtgtagtat gggtaccgaa tattcctcac ccttgcccga
     1501 atcctgcaaa attgacgaat gcagacgatt tggcacaggc gctattcaat aattgcttta
     1561 aggtgacaga cggtcctgac gcaccggatg ttgacttcat tgaattaaac aatcagctcg
     1621 ttatggtatt gacgaatgat tcactgacat ctaataatgc ttttgagaat tattccggag
     1681 ttgacttaag agcacctgaa ggtactgtcg attctttgta cagatttgaa ggatataaga
     1741 tatatcaggt tgccggttct gatgttacca atctggaact caatgaccct gagaaggcac
     1801 agttgattgc tcaatatgat cttaaaaatg gtgttacgca gatatttaac tggaatacta
     1861 ttgataaacc aaattctaat acaaacgaaa aatactggat tcctacattg aaagtagtgg
     1921 gtcaggattt aggtattaaa cataccttaa gcattacaga ggatgtgttt gctaagggag
     1981 accgaaaatt aatcaatcat aagcaatatt acttcaaggt agttgcttat gcttataata
     2041 attacgaaaa atttgaccct ataacaggtc taggtcaaag aacaccatat ctcgagggaa
     2101 gaagagtatt cacagcagtg ggcattccaa gaccaattat agatgtaaac caaaatgcag
     2161 agtatggtac tttggggttg gtatcccggg ttgacggcaa tggtacagga ggctttgatc
     2221 ttgaaataag tgatgataca cgatccttac tatttgagca atcatatagc ggtgacgtgc
     2281 catatctgcc tggcagaggt ccaatagacg taaagattta taaccctgtt gatacaaaga
     2341 aaggagagtt tatactcact tttgtagatt ctgatatgtc taataataaa cttgatgttg
     2401 gttgtggctg gactttgaaa aaagttgatg acccaagttt tgaggtttta tcagaggaaa
     2461 gtattgatca actcaacgag caggtaattg caaagtatgg tttttccatt actattggcc
     2521 agataccaga cgccggaacc acctttgatg ctaaaaacgg ctttgttgga accaggatta
     2581 aatatgctga gggagcagga caaaagtggt tggtaaatat acctaactca cctggtgtgg
     2641 ggcctttgca ttacataaag actaatgtgc taaaaacaca atggaatgcc cttgatccaa
     2701 aaggagcata cattaacgac cttaatattt atccttacat attgtgtgac tatgttgata
     2761 gtacgatggg tttcatctct ccggcatggc aggatttgac caataagact gcaattgcac
     2821 ctatgaaact ttctgagctg aataatgtag atatagtatt tacaaaagac aagagcaagt
     2881 ggagccgttg tgttgtcatt gaaacaacac ctaaaaagct tggtgaaatc aatacgccta
     2941 ttggcaatgc aaaacaattt gatttaagaa aatcaccttc agtaggtaaa gatgacaaca
     3001 atggcgatgg gctcccagat ccagatggag atggagatgg tatgggttgg ttcccgggtt
     3061 atgccgtgga tgttgagacc ggccagagat taaatatttt ttttggtgaa gcatctgcgt
     3121 atgcaaaggg cgcaccgttc gatgacaatg gtacatttac cggaggtgat atgatgttta
     3181 atccttccaa tcatacagta ttgaacaacg cggccggtaa tatttatgat gctatatttg
     3241 gtggattaca tacggtatat gtgacgaaat ccaagtatga tggttgtgac agtatcagaa
     3301 ccttattgaa ggaacctact accggattga agaaagtaca ggtaatacgg aaactgactt
     3361 ggacatttat gccattcttt gcgcctgaga cacagaccac aggaataagt aatggtatca
     3421 taccgaatga ttgtgttgta tctgcgcgag ttgacaacat ttaccatgta gccaatcaaa
     3481 gagtaacgga tcagtacaaa ggttatcctt catacaagat tactatttta ggagctaatg
     3541 aaacaactca gcttactgac tcacaacgag ttgagtcgtt agataatatt aatatcgttc
     3601 cgaatccgta ttatgcatat tcggcttatg agacatcgca gtttaccaat actgtgaaga
     3661 ttaccaattt gccaaataaa tgtacggtaa gcatttactc gttagatggt aaattcattc
     3721 ggcagtacaa acgagatgaa gtaccagggt cggcagctaa cagaccaaat gcaccattca
     3781 gtaattatca ggtaatacct gacctcaact gggatatgcg taacgataaa ggtgtgccta
     3841 ttggtagtgg tgtctatttg attcatattg atgcgccagg agttggagaa cgaacactta
     3901 agtggtttgg tgttggcaga cagtttgacc cgagtggatt gtaatatcag attaaatggt
     3961 ggagagtacc aaacggatac tctccaccta acaataaatt tagaaagaag cagaagaaag
     4021 tttaaattaa ccattatgaa gaaactactt tacacaatac tgaccgtttt cacgttttcg
     4081 tctgtctttg ccggtaatcc ggataggcaa ggcgaatctg gtgcagctca gttattgttg
     4141 aatccttggg caaggagtgc tggactacac actatgacaa ctgcaaatgt aataggtgtt
     4201 gaagcgatgc aattaaatat tgccggactt tctagggtca ataaaacaca ggtaatgata
     4261 ggcaatacgc aatatttgaa gggtactggc atcgggctca atgctattgg actttcacag
     4321 aagttaggag agaattctgc tatcggtatt cagttgatgg cagttgattt tggaaaaatt
     4381 ccgatcacta ccaccaacca acctgaaggg attggtgcta cttattcacc ttcatttttt
     4441 aatataggct tgggttattc atacacattc cagaataaga tttcagttgg atttcttgtt
     4501 agaggagttt ctgaaacact ggctgacata tcagcttttg gtattgctat ggatgccggc
     4561 gtacagtatg taaccggaaa gaaagatgag ttcaaacttg gtatttcact aagaaacatt
     4621 ggttcgccta tgaagtttgg tggtgaagga ctctctatag ctcaaaatgc gcctgaaggc
     4681 aattatcagc ttactttcaa tgtgagacct gccaactatg aattaccatc catgcttaac
     4741 cttggcgtgt cttatgattt tgatcttggc gagaatttga tattgaccgg aatcggtaat
     4801 tttacatcca attcatttgc acaggatcaa gtcgggttag gttttaattt tgcaataggt
     4861 aagtttttcc aggtgagagc cggctataag tatgagtttg ggcagtttaa cgaatcaggt
     4921 ctttatgctt ctatggataa aggtttgagt gccggagttt ctgtagagat tccgcttaag
     4981 aagaattcag aaagcagact tggtttggac tatggctaca gacatacaac acgttggtcg
     5041 ggtagtcata gtataggtgt gtcaataaac ctttagtagg gagcaacgtt taaagtaata
     5101 atataacacg tttccggacg attttcgccc ggaaacgttt caattttaat gaataacgaa
     5161 catatttttt aaaatgacag agtattctta tttaacacaa gaaggttacg acaaaataaa
     5221 agcggaactt gaagatttga agtcaaatgg cagaccagaa atagcaaggg caattgcaga
     5281 agcgagagac aaaggcgatt tgtcagaaaa tgctgaatac gatgccgcaa aaaatgctca
     5341 aggcatgtta gagttaaaaa tcaatgagct tgagaagttg ctgggcaatg taaggattat
     5401 tgatgaatca actgtcgatt tgtcaaaagt gtcattgttg tgtaatgtca ctattcgcaa
     5461 tattaagaca aaggctgaga ttacttacaa attagtatca gaaagcgaag ccgatcttaa
     5521 gcttaagaaa atatctataa aatcaccgat aggtaatggg ttattaggca agaaagtagg
     5581 tgatattgcg caagtacaga caccaaatgg cgtaatagag tttgaaatca tagatatttc
     5641 tttgtaagat atgagtacaa tcttttctaa aataattagc ggagaaattc cgtgttttaa
     5701 agtggcagaa gatgaacagc atcttgcatt tcttgacatt aatcctgttg catttggtca
     5761 tacgttggtc attccgaaaa gagagataga ttattatttc gacctagatg atgaagaaat
     5821 agctcgtttg atgattttca gtaagaaagt agctttagcc ttaaaatctg ttgttccatg
     5881 caccaagatt ggaatttctg ttataggtct ggaagtgcct cacacacacg tacatcttgt
     5941 gcctatcaat gcaattgcgg acatgacatt tgggaaaaaa attgaaattt ccattgatga
     6001 attgacaaaa cttgcgagcc agttagcgga taaaatcaag atttcttaag gcgggtttca
     6061 ttatttttta caaattcttg ccagccactg tattttttac ctgaagtagt gagaagttta
     6121 ttttgtgaat aatggcatac ggctacggct agtgcatcag tagcatcgag tttttcgtaa
     6181 acctgattat tcatatcaac tagatgaggc aacatacgag ctacttgttc tttggcagca
     6241 ttaccattgc ctgtaatcga ttgttttact tttttgggtg aatattcagc aatagtgagg
     6301 cccatgttga tggctgctgc gatggcaacg ccttgtgcgc gccctagttt tagcatcgac
     6361 tgaacattat tggcataaaa tggctcctcg atggcgagtt gttttgggtg aaatatttct
     6421 ataagtttac ttatttctcg atgaatatgt aataattttg tttgttggtc tttatatttt
     6481 gaaagattga tgacatccaa cgacattact tttggtggat cgcagatggt ttctataatg
     6541 gcaaatccta agaagttagt tcccggatca acaccgagta tcttgtatga aatatcttta
     6601 ttcaaatttg taaatattaa gccttcaaat atacaacatg gtaagacagg tatgtggtat
     6661 aaatgactaa ttttaacatg tattataaca aaatcgcatg agacccagac aaatcagttt
     6721 atttgtatta tttgtgtctg tagcattgtt tgtgatattg attgtttcca ggtttaccta
     6781 ccccgatgcc gatattgttc aatattatag agaccggacg ctttactata gatacttggt
     6841 attggcaata ttgcttatgc ctgtcaattg gtggttagaa tatttgaaat ttgttcaaat
     6901 gtcgcattta gaccaggtga atggaagggt agttttgata aaatctttgc ttagcggtat
     6961 atttttatca gccatcacac cggttaagtc aggagattat ggcggccgat tagttttcat
     7021 aagaaaagac gactgggcga ttgctttgaa tgccgtattt tggggaaatg gttgccagct
     7081 tttatggctt atggtatgtg ggctgttggg attattatat ttgagtacag tattgtcaga
     7141 aaacttgctt caggctaaga caagtcttgt cgttgtctta attgttgtta tgctaatgtt
     7201 aatcattgta tggttttact tgaggattgc gcttgtgagg aagttagtct tttacttacc
     7261 tttcaaagaa aaactaagaa aagtttatta taattttttg aagattaaaa atcgaaatga
     7321 tttgtataaa actttgataa tcgctgggtt gcgatatttt acatatatca tacaatccat
     7381 gctgttaata agcgttttta ataatacaat agctttcagt ttgttgctta gtggagtaat
     7441 gacggtattt tttattcagg ctattttacc tgtcctgagt tttttaggct tgattggaag
     7501 aacgggaata gcgatatttg tgttaaataa aataggagtg agtgaaatgg acagtggagt
     7561 gtcgagtatt atgttgtggg ttgtcaatat ttttattcca tctgtggccg gtttatattt
     7621 tttgttcagg aaaatgataa ataagcaaaa tgaaaaaaag gaactggata ttagttagta
     7681 caatgatggt attgcttgtc actgccggat tcaggccgac agtaaagccc gacgaattgt
     7741 atgatccgtg tgcgatcaat aactggacat ttaccggagg tgaggaaatt acctacaaaa
     7801 tatactacaa ctggaatttc atgtggttgg ctgcaggtga agctgtcttc agagtatatg
     7861 atgaaggcga cacttaccat ctcagtgtca aagggactac atacaaatca tacgagtggt
     7921 ttttcaaagt gagggatagt tatgatacat gggtagataa gaccacacta ttgccaaagg
     7981 tagccatacg cgacgtttcg gagggaaatt ataagcttta tgagaaaatg attttcgatc
     8041 aggagaattc aaagatcact tcttatagag gcaatacacc taaagatgca gtaaagaaag
     8101 agttcagaat caatgattgt atgcacgatt tgatatccat tatctatcat gctcgcaatc
     8161 tggagtatga aaagtttgcg aaaggacaag aataccctat caaaatattt atggacaaag
     8221 aagtatggcc tttgagtgta aagtataaag gcagatacaa gcaggtgaca gtcaaaggag
     8281 gcaccaagta caaagcattc aagttttcac ctgatgtgat aagtggagaa gtatttaagg
     8341 agggtacaca aatggacatt tgggtgtctg acgacaacaa tcgcatacct gtatatattg
     8401 aatcgccggt aagtgtgggt tcagtaaaga ttgtattaaa agattataag ggattgaagt
     8461 accctctaac tgccaaaatt tgagatacca agcagtgggg aacagcatgt tgaagaagat
     8521 attatacatt gcagtcggtt taataattgt atttgccatt gtcaaggtga atgatattta
     8581 ccacagcttt actgccagaa aaactgccga agagtcggta gtactgctgg agagtatccg
     8641 caaggtgacc aagctggtag ctgtagaggg atatttttca gaggtatatg attataaaga
     8701 ttattatggc tttgatattt caccactcag gaagaaagcc ctgatacgtg tgaaggccaa
     8761 agtgtcggta gaatatgatt ttgacaagct aaagataact gccgacgatt cgaggaaaat
     8821 aatccatatc agttcaattg gaaagccaca gatattgtca gttgaccata agcttgatta
     8881 ctatgatatt tctcagggca ccttcaattc atttaccagt gaagactaca accggatgaa
     8941 ccaaaatgcc aaagatttca ttataagtaa ggtgaaggaa agtgatttga tgaatcaagc
     9001 cggtgaacaa atggatggta tcattgagac gataaagaca ttgactgaaa gcagaggatg
     9061 gcaaatagtt gtggagaaag aaccaacgat acaataaatt aaataatttt accctaatta
     9121 tcattttaca aaaacttaat ataaaaataa catgtcttac aattatcagg aagctgtagg
     9181 agagctaaaa cgaagaggat acctggcagt tgatgttgac cctactcttg acttcgaagc
     9241 ggagatcatc agactcaaga aagaaaagaa cgctgtcatt ctagcccatt attatcagga
     9301 ctcggagatc caggatcttg ccgattttgt tggagacagc ctcggcctat cgcaggaggc
     9361 agccaagacc aaggcagata tgattgtttt tgcaggtgta cattttatgg cagagactgc
     9421 taagatgcta tcaccacaga agaaggtagt gctgcccgac ctcaatgcag gttgctcgct
     9481 ggccgattcg tgcccggctc ctttatttag taagtttaaa gaaaaatacc ctgaacatat
     9541 agtagtgagt tatatcaact gttcggcaga gctaaagacg cttactgata tatgctgtac
     9601 atctaccaat gcggtacaca ttattgaaag cataccgaag gataaacaga taatttttgc
     9661 tccggaccgc aatctaggga agtacctgat aaagaaaacc ggtcgcgata tggtgttgtg
     9721 ggaaggtgcc tgtatggtgc atgagatatt ctcaaaggaa aagattgtag cattgatgca
     9781 tacacatcct gaagcgaaac ttattgccca tcctgaatgt gagcctcaca tcctggagca
     9841 tgcacactat attggttcta caagtggtct tctcaaattt tctaaaacag acccagccaa
     9901 agaatttatt gtggccacag agcccggtat catctataac atggagagag catcacccga
     9961 caaagtattc ataccggctc cacctgacaa ccattgtgct tgcaatgaat gcccacacat
    10021 gaaacgcaat acacttgaga aactgtacct ttgtatgaaa tacgaattgc cggagatttt
    10081 gctacctgac tgggtaattg atcaggggaa gaaatcaata gagagaatgc ttgatatttc
    10141 agctaaggcc ggactttgat tttgatatag aactttttta gagtattgat tgtttgaatg
    10201 gaaaatagaa attgaataaa tatgatgatg ttgtatgtta ttagtgccgt attcggtgtt
    10261 gtaggtatgt tggtgagcgc aaggcttaaa agcaaattta gtcattacgc cctagtacct
    10321 gttaccaatg gtatgactgg aagagatgtg gcagaagcaa tgcttaaata ttataatatc
    10381 catgatgtac aggtaacagc agtagaaggc acccttaccg accactacaa tccgcaggac
    10441 aagacagtaa accttagtca ggatgtgtat tatggcaacc atgtatcagc agcggctgtt
    10501 gcagcccatg agtgtggtca tgccgttcag catgctactg cttatacagc acttactatg
    10561 aggtcaagac ttgtgccgat tgtgagcctt tcagcgaacc ttcagcaata tttgtttatg
    10621 tttggtgtag tgggtatggc atcatttaac agtccgttgt tgcttataat agctatagcg
    10681 gcctttggtg ttactgcgct tttcagtctt atcactttgc ctgtggagtt tgatgcaagt
    10741 agcagggcac ttgtatggct taaccatact aacgtagtaa gtggtgcaga atatgatggt
    10801 gccaaagatg cactcagatg ggcagcgctt acttacgtag tagccgcgct ggctgcggta
    10861 gcccagttgg tgtaccttgt gatgatgttt atgagcagaa gagactaata ttcataaaat
    10921 aatctaatat gaaacaggaa tggtgtaagg ctgttcctgt tttatcattt taataaaatg
    10981 ctttgtatat ttgcaaatta atattcaccc tattatgaca gaacaggaaa ttcaaatcat
    11041 cacgcagaaa gctaaaaact caatggatca ctcattggac cacctccaga aggaactatc
    11101 caagctgcgt acaggaaagg catctaccac tatgctagaa ggcatcctcg ttgattatta
    11161 cgggtctcca acattgctca atcaggtagc caatgtggca acttctgacg cccggaccat
    11221 ttctatccaa ccatgggaga aaaagatgct ttcacatatt gaaaaggcaa tctttgaggc
    11281 caacctggga gtgacaccac agaatgacgg tgaagtgata cgcattatca ttccgccact
    11341 tacagaagat aggagaaagg acctggtcaa acaggctaag catttaggcg aagaatcaaa
    11401 ggtaggattg agaaatgcca gacgcgatgc tatggaggtt tttaagaagg ccatcaagga
    11461 cggtttgtca gaagatattg gcaaaagaaa agaggaagag actcagaaaa tgctgaattc
    11521 ttatgtagaa aaaattgata caatagtgag tgccaaggaa aaggaaatta tgaccgtata
    11581 aaagtagtcg tttttctgct tattttggta aaatttgttg tagcacaagt taagtgaagt
    11641 acactacagt tttaatccag tgtaaagatt ctaattgtat cggccgtaat ctccatccag
    11701 catttgtaaa tgtactgaat gttattggcc tggtctagaa atgtaggtat cgggtatgat
    11761 tttactcgca tattgttaag gtcgagtaca tctatgtagt cctcttgtcg gtaatagatt
    11821 ctactgttag taaaatactg tatttcttca tcaggcaacg gaagtgtctg gatataacta
    11881 ccaaaatcat caaagagcat ccagccgaaa tcgggagcac gcatcaggag aaaattatcc
    11941 tgctcccata tttgtccggt aaatgccgat ttccctacca ggcctgtcag ttcattgctt
    12001 ttacggatta tttttccaaa agaatcaatt ttaatgagcc tgaaagtctc catattataa
    12061 aaccagatgt tgttgtctgc tccaagacct atagccgtat tggtcaaata gccaaaagtg
    12121 cgcagattaa actcagattg aagtgtaagg tttctgtcga gtatctgtac tatgccgaag
    12181 tcggcataat ataccaagat actgagtggg ttattgatgt caataaccgt aaaacttccg
    12241 tagcgcactt cggtatattc tgccattttt gtaccttttg aatcatactg aagcaaacga
    12301 ggttcattgg tagctacgta tatgttgcgg aatctgtcta agagtactag ctctgtgttt
    12361 ggtatattta atttagtgac tatcaaagaa ctgtcttggg cagaaattgt agtgataaac
    12421 gccaaaaaca tacaaagcac gaaagcgaat aattttctca tgatcgattg cagaatattt
    12481 tggtatcctt ttcccaggaa tgcaactcaa taccatcggc attaatgacg gcaaacgtat
    12541 tataacgaat ccagtcaccg aggtttacat agcggctgtt ttctttagtg aaataaaaat
    12601 caatgggtaa atgtctgtgt ccaaagatga aagcatcata gtgctgtgta gcaagtgttt
    12661 ctttacagaa ttgcaccagc cattccctat cttttcctaa aaaatggtcg gggtcggtat
    12721 tggcattccg gcttttagct gaccaatagt tggcaaggcc gaaagaaaaa ttaggatgta
    12781 gtcttgcaaa tagccactga cagatcggat tggcaaatac ttttttgatg aatttatagc
    12841 catgatcacc ggggcccagc ccgtcaccgt gtccaataaa atactttttt ccgaagaatg
    12901 ttttcatgat gggctgtttg tgtaccggaa taccaagttc gtgtgtcaaa tagccgaaca
    12961 tccacatgtc atgattgccg gtgaatactt cgatgttgat accacggtcg cgcaatgagg
    13021 ccagttttcc aagaagcctt acaaaacctt tgggtacagt atatttatac tcaaaccaaa
    13081 agtcgaacaa atcgcctacc aaaaaaatat ccgaagcatc tgctgcagcc atatcaagcc
    13141 aacgaacgat gcttttttct ctttccagac tagtagagcc ggcatctact ccgaggtgaa
    13201 aatcggtggc aaggtaaatt ttttgcatat tgtcagacaa gctatacctt ttccattaca
    13261 gagccacacg aattgcatgt tctgagatct tctgagttca tgaaatggtt catagccggt
    13321 ggcagttgat tgacgatatc ctgcatttcg aaacttgcct cgtggagttt gtttccacat
    13381 ttttcacaaa accacatcag tttgtctatt tcttttggtt tgcgtgtcct ttctatgaca
    13441 agtccgatgg tattggcggg cctttgaggg ctgtgaggcg tatgtggagg aagtaagaac
    13501 atgtcacctt cgcggatatc gatggtttcg ggcttgccgt cgggatgaat gatttttacc
    13561 tgtatgtcgc cttcaagttg atagaataat tctt
//
LOCUS       DAOQQY010000043        13557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1522_length_13557_cov_5.370834, whole genome shotgun sequence.
ACCESSION   DAOQQY010000043 DAOQQY010000000
VERSION     DAOQQY010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13557
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1522_length_13557_cov_5.370834"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PLE23_03855"
     CDS             <1..395
                     /locus_tag="PLE23_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82431.1"
                     /translation="DLPYLEVGPYHTNTVAGLDLAAQILRKRNNPNKQVFMITDGKPT
                     CIKKGSKYYQNSFGLDRMIVNRTINKGIQLRKMGVPVTTFMIASDPYLVQFVRQFTQA
                     NNGKAFYSGIDGLGNFFLDNYLANKKRK"
     gene            598..1428
                     /locus_tag="PLE23_03860"
     CDS             598..1428
                     /locus_tag="PLE23_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82432.1"
                     /translation="MPVTGQAISKHSMYENAKMMLSLSGYDTISVAYKEFNEQFLNEL
                     HLPGSFSNKFDSLECVSFLYPPDSSFRIMSWQAPVARDKYAYSCYIQTHDSLFVLKPS
                     PSYFNHSSFNTLELRHQAWYGCLYYGIKSFVHDRQTKYVIFGYQQPGPFSARKIMDVL
                     WFDNGVPVLGSLVFCNKKTKQCNTRMTLEYDSRATIRLNYDPEYGLIIFDHLIKGASP
                     DMYGQTVNMTDGSYDAYKLKKNGTWEFVDMLWHDKQKEPPTDKPKKSQEQGKKDIFGR
                     "
     gene            complement(1432..2478)
                     /gene="rfbB"
                     /locus_tag="PLE23_03865"
     CDS             complement(1432..2478)
                     /gene="rfbB"
                     /locus_tag="PLE23_03865"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HMX82433.1"
                     /translation="MKTILITGGAGFIGSHLVRLFVNKYPEYKIINLDALTYAGNLEN
                     LTDVADAPNYTFVKGDICDRQLIADLFAQYRFDGVIHLAAESHVDRSIEDPDSFLHTN
                     VYGTLNLLAQAKKSWTDTSGKLFYHVSTDEVYGSLGDTGYFTEETAYDPRSPYSASKA
                     SSDHLVRAFFHTYGLPVVISNCSNNYGPNQFPEKLIPLVINNIKNKKSLPIYGDGKYT
                     RDWLYVIDHAQAIDTVFHKGKNGETYNIGGNNEWKNIDLVTKMCNIMDKMSGNAPGTN
                     RQLITFVKDRPGHDKRYAIDASKIKRELGWEPSVLPDSGLEMTIRWYLDNITWLENVT
                     SGSYLEYYEKHYAR"
     gene            complement(2501..3448)
                     /locus_tag="PLE23_03870"
     CDS             complement(2501..3448)
                     /locus_tag="PLE23_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMX82434.1"
                     /translation="MSKKRVLITGAAGFLGSHLCERFLSEGFEVIGMDNLITGSLDNI
                     AHLFPDKDFTYYHHDVTKFVHVPGELDYILHFASPASPIDYLQMPIQTMKVGSLGTHN
                     LLGLAKEKKARFMIASTSEVYGDPLVHPQTEEYWGNVNPIGPRGVYDEAKRFQEALTM
                     AYHRYHGLETRIVRIFNTYGPRMRVNDGRVLPAFFSQAIRGEGLTIFGDGTQTRSFCY
                     VDDLVEGIYRLLMSDYALPVNVGNPDEITMNYFAEEILRLVNNPNAFIDYRPLPEDDP
                     KQRQPDITKARNILGWEPKVQRAEGLARTYEYFKKVVTV"
     gene            complement(3482..4606)
                     /locus_tag="PLE23_03875"
     CDS             complement(3482..4606)
                     /locus_tag="PLE23_03875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMX82435.1"
                     /translation="MHKIQMVDLHNQYLKIKSETDAAIQDVLDQGYYINGAPVHKFSE
                     NLATYLNVAHVIPCANGTDALQIALMALELQPGDEVITSPFTFVATAEVIALLKLKPV
                     FVDIDPDTYLIDVNKIEAAITPRTRCIIPVHLFGQIANMEKINEIAQRHNLYVVEDNA
                     QAIGADYTFANGNQVKAGTIGHIGCTSFYPSKNLGCYGDGGALMTNDGKLAEKIKVIC
                     NHGSKQKYYHEEIGVNSRLDSIQAGILDIKLKHLDQYITERQRAAEYYDRHLSDIDGI
                     QIPVRTKHSTHVFHQYTLFVDKDRDRLKDSLQESGIPSMVYYPVPLHVQAAYTGDGYK
                     AGDFPHSEKAARHVLSLPMHTELSDEMLGYICTKIREAFI"
     gene            4797..5477
                     /locus_tag="PLE23_03880"
     CDS             4797..5477
                     /locus_tag="PLE23_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82436.1"
                     /translation="MSEIYEGNVFHIALSYGYQLPLADMKDIYGNNFNVGLSFEDQLK
                     NNYLFGLEGQFLFGKNVKPDVLINLKDDQGIIYGNDKTYGSVVLRERGWYIQAYAGKI
                     IPLNNPRSGVKLKLGAGYFQHKIRLQDDLNTINQIKAEYRKGYDRLTFGLGLSPYVGY
                     QYISNDKRINYNIGIESIIGLTEGRRDYQFDTMSTFRSKRLDVVIGIKAGWILPFFTD
                     VPAESYYY"
     gene            5482..6738
                     /locus_tag="PLE23_03885"
     CDS             5482..6738
                     /locus_tag="PLE23_03885"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HMX82437.1"
                     /translation="MTSVLYYSAIFLYCFVIRVAVLFNAKAALWGAGRKNWRTNLKLA
                     CAKLSGKIVWIHCSSLGEFEQARPIVDGIKSECQGVNVVVSFFSPSGYTIRHNYKYAD
                     MVVYLPCDLPGNAAYFIKTLKPNLAVFVKYDLWYGYLKVLFQMNIPAILVSAIQHKAL
                     TGWSGALYKKICYRLLDKIYVQDELSMTNIRAAVNREVIVAGDTRIDSVIRFSGVTDH
                     QLDHDMKRFVGDDSCLVCGSTWPADQDLLIPLIKDEIFKNWKAIIAPHEMKKDDLENL
                     KKQFGEKAIFYSEFERYDQTHTVLIIDSIGHLSKIYRYATLAYIGGGFGNGIHNTLEP
                     AAFSLPVIFGPKFKKFSEAVNLTKDKAFAPINNQNELFSTFIYYNKVENRKNAAEAIR
                     AYMNSNKGASGLILLDIKLYYLGNEN"
     gene            6754..7752
                     /locus_tag="PLE23_03890"
     CDS             6754..7752
                     /locus_tag="PLE23_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HMX82438.1"
                     /translation="MSFYKNVLGEFRNKKVLIAGDIMTDRYLTGTVNRISPEAPVPVV
                     QLTHADDRLGGAGNVALNVKAYGAIPILLTVVGEDDDGLKLLKQLNSLGIEHKFICKS
                     VERRTTVKTRIVSQNQQMMRIDAEDTFELTHHEKQVVIAQYVNALKETKPDVIILQDY
                     NKGFFTPELIQVITRYAADNNIPVTVDPKKANFLDFKGVTIFKPNLKEVKEALNTEIK
                     PEIGDLKNASGHIRDILGNKITVITLSENGLFIDDGVDAKIYPTTPRNISDVCGAGDT
                     VISTLSLGIASGLSAYELAVMANVAGGQVCERPGVVPVDPDQLILELSETLYSEKT"
     gene            7846..10242
                     /locus_tag="PLE23_03895"
     CDS             7846..10242
                     /locus_tag="PLE23_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82439.1"
                     /translation="MKLKFLQTRFFSAFVFVVASMWSMTAFAQPANDLCSNAIELTIA
                     ADEASAVPVAGTTVGTVDGATTPGPKVCSANFYRDDVWYKVTIPSGVAAEAVTIAVNR
                     NVTGGMTDCGMAIYVSNACDTSNAAYLCQNFTGNGSFRFSKICTGEDKELLIRVWSGA
                     GTAANWAQGQGAFEISAFYSDGEQAIWGNNGEGSFNGGLGGWTTTLDAKCPGFELWRW
                     SNTTYCTNGAFSSGGGTITSLTVCNGAMCFDSDYYDNNGQSTGIGTGPCTAPQEGSLV
                     SPAIDLSVFPDVTGVNLVFSQATRQFQSEYYIELSLDEGTTWNTIEINTAEEDPDNYA
                     VNGPHVNNNRRVFLPGAAGKPNVQVRFRYVGNYYYWIVDDVKITERESYNLRVDPFYA
                     IAPNKQWQRDQLESFGGLADISNNGSKGTTNTALSLEIKDAASTTIFTDELSYGTTVA
                     DSTYENVPMTGTFTHPNDDVTTYTGTYALTSTEPDFDYTDNSRNFSWSVTDSIMAKDG
                     STTLGNGIRAGSDYNYTWGNIYHIVNNKSSLNGKTYHCNYAGVGIANPDDLAGATIFV
                     YLYKWDNANGDDVIQADERTTVGFNQYDFPVGEAANTLYTLPIFDYATFEHGVALESN
                     TDYVLVVEYTTPPDQLELPCFINSDASIDYGAQTVRSTTPGFGPVRYNHALDVGNTGE
                     MTANTFTGGTTPVIRMFLSTTTISTNEVKLDDANKIVVAPNPVKDVLNVKIDLVENAK
                     EATIEVVDINGKILNTRTIENFKSTTETFDVSNLVNGNYFVNYKSKAGVRSVKFIVAK
                     "
     gene            complement(10433..13153)
                     /locus_tag="PLE23_03900"
     CDS             complement(10433..13153)
                     /locus_tag="PLE23_03900"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMX82440.1"
                     /translation="MTLSTRYLPNDIENKWYTHWMEHNYFHSVPDDREPYTIVMPPPN
                     ITGVLHMGHLLNNTIQDILIRKARIDGKNACWVPGTDHASIATEAKVVQLLRKQGIKK
                     SDLTREEFMRHAFEWKEKYGGIILEQLKKLGCSCDWDRTRFTMEPSLSNAVTGVFVDL
                     YKKGKIYRGLRMTNWDPEAQTVLSNEEVIYSEEQSRLFHVRYRIEGTDEYITIATTRP
                     ETILGDTAIAVNPDDERYAHLKGKKAIVPIVNRVVPIIFDRYVEMDFGTGALKVTPAH
                     DINDYEIGKQHNLETIDILNPDGKIADIAGHYIGMDRFEARKAIVKDIDALGQIAKIE
                     DYRNKVGRSERSNAIVEPRLTLQWFLNMKEFANTALSSVEQGNIRFFPDSFINMLRTW
                     LAEDNIKDWCISRQLWWGQQIPAWYIGEHVFVATTAEDALSQAKEKLDNHSLQLTDLI
                     QDEDVVDTWFSSWLWPISVFDGFERQDELKYYYPTQVLVTGWDIIFFWVARMIMAGYE
                     WSGDLIGTELSAAKGNFPFKDVYFTGMVRDNKRQKMSKSLGNSPDSLELMAKYGADGV
                     RFGLLSSASAGNDIIFDAPFDNETKQIINESKLCEQGLNFCNKIWNALRLIKGWQVSE
                     DIKDINQKEINTIAVKWFENKIAQKFEKYEENYKDYRLSEITVDLYSFIWDDFCGWYL
                     EMIKPPFGESIDADSLEKTVQIFEQLMTMLHPIMPFVTEEVWHQLRDRVPGDNCIVGK
                     KPELKPFDLHFIRQIDAVKEIITKVREVRNSKGVKMADQLPFFANPSPQNTDWLANEG
                     IKNMLKKMANLSSIEITENEPEKCISIVSGADKYYVLLDIEIDIEAERERIEKELEYQ
                     RGFVASIEKKLSNERFVSGAPAAVVDKERQKMNDGLSRIGFLEDSLRNLK"
     gene            complement(13171..>13557)
                     /locus_tag="PLE23_03905"
     CDS             complement(13171..>13557)
                     /locus_tag="PLE23_03905"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HMX82441.1"
                     /translation="KADFSKNAELSEAGEARSERLSKILRKAPVADMVYSSDFIRTIN
                     TIKPYCLSKNINWQLYNPANQAELAAKLTPYKRILISGHSNSVPALINLLTGSNMAEL
                     DEDDYSNLFFVKIPKNGPKTIYIFKY"
BASE COUNT         3857 a         2691 c         2842 g         4167 t
ORIGIN      
        1 aagatttgcc ctacctcgaa gtaggacctt accataccaa cacagttgct ggcctcgacc
       61 tggcagcaca aatattgaga aagcgcaaca atccgaacaa gcaggtattc atgattaccg
      121 atggcaaacc tacctgtatc aagaaaggca gtaaatatta tcagaatagt tttggactcg
      181 acaggatgat tgtcaatcgt acaatcaaca aaggtatcca gctgcgtaaa atgggtgtgc
      241 ctgtcactac ttttatgatt gcgtcagatc cttaccttgt ccagtttgtc aggcagttta
      301 cgcaggccaa caatggcaag gcgttctatt cgggtattga cgggcttggt aattttttcc
      361 tcgacaacta tttggccaat aaaaaaagaa aataaaaaag cccgtacaaa tgtaagggca
      421 agcaatttag gattataata tgtatatata aatgggattc gtgtttgtga tatttgttaa
      481 gccaaaattc ataccaaagt cagcactata aaaaatatct attcgttata ctcgaatatg
      541 cttaaaaatt ctatcaaatc ttttttcatt ttattagcat ttttcattta tgaaattatg
      601 cctgttaccg gtcaggctat ttctaagcat tcaatgtatg aaaatgctaa gatgatgctc
      661 agtctttcgg gttatgatac tatttctgtc gcctataaag aattcaatga acaattcctg
      721 aatgaactac acttacctgg ttcttttagc aataaatttg attcacttga atgtgtttct
      781 tttctatatc cgcccgacag tagtttcaga ataatgagtt ggcaagcacc agtagcacgg
      841 gacaaatacg cctatagttg ttatattcag acccacgaca gcctgtttgt tttaaaacca
      901 agtccttcgt atttcaacca ttcatctttc aatacacttg aactacgcca tcaggcctgg
      961 tatggttgtc tttattatgg catcaaatca ttcgtgcatg acagacagac taaatatgtc
     1021 atatttggtt atcagcagcc ggggccgttt tctgccagga aaataatgga tgttctgtgg
     1081 tttgacaatg gtgtaccggt attgggtagc ctggtatttt gtaataaaaa gacaaaacaa
     1141 tgcaatactc gtatgacgct tgagtacgat tcgcgtgcta ccatcaggct aaactatgac
     1201 cccgaatatg gcctgatcat cttcgatcat cttataaagg gagctagccc cgacatgtat
     1261 ggccaaactg taaatatgac ggatggcagc tatgacgcct acaagttaaa gaaaaatgga
     1321 acatgggagt tcgtggacat gctgtggcac gacaaacaga aggagccacc aaccgataag
     1381 cctaaaaaat cgcaagaaca aggtaaaaaa gatattttcg ggcgttgatt tttatctggc
     1441 gtaatgcttc tcgtaatatt caagatacga gccagatgtt acgttttcga gccaggtaat
     1501 attgtcaaga taccatctga tggtcatttc gagcccacta tccggtaata cggatggttc
     1561 ccagccgagt tcacgtttaa tttttgatgc gtcaatggca tatcttttat cgtgcccggg
     1621 tctgtctttt acaaatgtta tgagttgcct gttggtgcct ggagcatttc cggacatttt
     1681 atccatgatg ttgcacattt ttgtcacaag atcgatgttt ttccattcat tgttaccacc
     1741 gatgttgtag gtttcgccat ttttgccctt gtggaaaaca gtatcgattg cctgtgcatg
     1801 atctattaca taaagccagt cgcgcgtgta ttttccgtca ccgtatatgg gtaggctttt
     1861 cttgttttta atgttgttga taacaagcgg tataagcttt tctggaaact gattcggacc
     1921 gtagttgttc gaacaattgc tgatgaccac cggcagacca tacgtgtgaa aaaatgcccg
     1981 tacaagatga tctgacgatg cttttgacgc agaataaggt gatcttgggt cgtaagcagt
     2041 ttcttcggtg aaatatccgg tgtcgccgag cgagccatac acttcatccg ttgatacgtg
     2101 atagaaaagt ttgcccgatg tgtctgtcca ggatttcttc gcttgcgcca ggagattgag
     2161 cgtgccatat acgttcgtgt ggagaaaact gtccggatct tctatcgacc ggtcaacatg
     2221 tgactcagct gcgagatgga taactccatc aaatctatat tgagcaaata agtcggctat
     2281 taattgcctg tcacaaatat cgccttttac gaaagtataa ttgggcgcat ctgccacatc
     2341 agtcaaattc tcaagattgc cggcatatgt aagagcatcc agatttatga ttttatattc
     2401 cggatattta tttacaaaaa gccttaccaa atgcgaaccg atgaatccgg ccccgcctgt
     2461 tattaatata gttttcattc cttaattttt gcagcgttgt ttatactgtc actacttttt
     2521 taaaatattc gtatgtcctg gccaagcctt cggcgcgttg tacctttggt tcccagccga
     2581 gtatgttacg tgctttggtt atatcaggtt gcctttgttt cggatcatct tcaggtagag
     2641 gcctgtaatc aataaacgca ttcgggttgt ttaccaggcg caatatctct tctgcgaaat
     2701 aattcatcgt tatttcatcc ggatttccaa cattgaccgg taaagcatag tcgctcatca
     2761 acagcctgta aataccttcg accaggtcgt caacataaca gaacgaacgt gtctgtgtgc
     2821 catcaccaaa aatggtgaga ccttcacctc gaatagcctg tgaaaaaaat gccggcaaaa
     2881 cccgaccatc atttacacgc attcgtggtc cgtacgtgtt gaatattctt actatacgtg
     2941 tttccaggcc atgataacgg tggtaggcca tcgtcagggc ttcctgaaag cgtttggcct
     3001 catcatacac accacgcggg cctatcgggt taacattgcc ccaatattct tccgtctgag
     3061 gatgtacgag tggatcgcca tatacttctg atgtggaagc aatcatgaat cttgctttct
     3121 tttcttttgc cagaccaagc aggttgtgcg taccgagtga acctactttc atggtctgta
     3181 taggcatctg gaggtaatct atcgggcttg ccggtgatgc aaagtgcagg atgtagtcaa
     3241 gttcgccggg tacatgtacg aatttcgtta catcatggtg atagtaggta aaatccttgt
     3301 caggaaacaa atgcgcaata ttatcaagcg atccggtaat cagattgtcc atcccgatta
     3361 cttcaaatcc ttcacttagg aatctctcgc acagatgcga acctaaaaat ccagctgctc
     3421 cggtgattaa tactcttttt ttcgacatca aaatatttga atgttataat gattgaatgt
     3481 gttatatgaa tgcttcgcga atttttgtac aaatataacc gagcatttca tccgataatt
     3541 cggtgtgcat cggtagagat agtacatgcc gggcagcttt ttctgaatga gggaaatcac
     3601 ctgctttgta accgtctccg gtgtaggcgg cctgaacatg caacggtacc ggataataca
     3661 ccattgaagg tatgcctgat tcctgaaggg aatctttgag acggtctcta tctttatcta
     3721 cgaataatgt atattggtga aatacatgtg tggaatgttt agttctcacg ggtatttgta
     3781 ttccgtctat gtcgctgagg tgtcggtcgt aatattctgc tgcgcgctgt ctttcagtga
     3841 tatattggtc cagatgtttt agtttaatgt caagtatgcc tgcttgtata gagtcgagcc
     3901 ttgagttgac acctatttct tcgtgataat atttttgttt agacccgtgg ttgcagatta
     3961 cttttatttt ttcagctagc tttccgtcat tcgtcatcaa tgcgcctcca tcaccgtagc
     4021 aaccgagatt ttttgaagga taaaaagaag tacaacctat atgtccgatg gttccagctt
     4081 tcacttgatt gccgttggcg aatgtataat cggcaccgat ggcttgtgca ttgtcttcta
     4141 ctacatataa attgtgtctt tgggcgattt cgtttatttt ttccatgttg gcaatctggc
     4201 caaacaaatg taccggaatg atacatctgg tacgcggcgt aatggctgcc tctattttat
     4261 ttacatcaat gaggtaagtg tccggatcaa tatctacaaa tacaggtttc agcttaagca
     4321 aggctatcac ctcggctgtt gctacaaaag taaatggtga agtaatgacc tcatctcccg
     4381 gttgaagctc aagagccatc aatgctatct gcagtgcatc ggttccgttg gcacaaggta
     4441 ttacatgagc aacattcagg tacgtggcaa ggttttcaga aaatttgtgt accggagcac
     4501 cattgatata atagccctgg tctaatacat cctgaatggc agcgtcggtt tcggatttaa
     4561 ttttgagata ttggttgtgc aagtcaacca tctgtatttt gtgcatattc ataatttggg
     4621 tgcaaatata gtttttttgt tggtgcagat ggttaaatca ccggataatc tgttagcttt
     4681 gcctgaataa aatacattgt acttaaccgt gttgttctat gtcttttttg aaaaatatta
     4741 ttgtcgcctg tcttttgttc tttggcactg ttataagttt ggcacagaaa gccaaaatgt
     4801 cggaaatata tgaaggcaat gttttccata tagccctcag ctacgggtac caattgcctc
     4861 ttgccgacat gaaagatata tatggtaata attttaatgt aggactttca ttcgaagacc
     4921 agttaaaaaa taattatctg ttcggcttag aaggccagtt tttgtttggc aaaaatgtaa
     4981 aacccgacgt attgataaat ttgaaagatg accagggtat catttatggc aatgataaaa
     5041 cctacggttc ggttgtgttg cgcgaacggg gttggtatat tcaggcttat gccggtaaaa
     5101 tcattccact aaacaatccg cgttcgggtg tcaaactcaa gttgggcgca ggatattttc
     5161 agcataaaat aaggctgcaa gacgacctca atactatcaa tcagataaag gcagagtaca
     5221 gaaagggata cgaccgtctt acttttgggt taggtctgag tccatacgta ggctaccaat
     5281 acataagcaa cgacaagcga atcaattaca acattggcat agaaagcatt attggactta
     5341 ctgaaggaag acgcgattat cagttcgata ccatgagcac attccgcagc aaacgattgg
     5401 atgtagtcat tggtattaag gctggctgga ttttaccgtt ttttacagat gtaccagccg
     5461 aatcatatta ttattaagcg tgtgacatcc gttttatatt attccgcaat atttctatac
     5521 tgctttgtta tcagagtagc tgtattattc aatgccaaag ccgctctgtg gggcgcaggt
     5581 aggaaaaact ggaggacaaa tcttaagctg gcttgtgcga aactcagcgg caaaatagtc
     5641 tggatccatt gttcgtcgct tggagaattt gagcaggcaa gacctatcgt ggatggtata
     5701 aagtccgaat gtcaaggtgt gaatgtagta gtcagttttt tttcaccctc cgggtacacc
     5761 attcgacata attataaata tgcagatatg gtggtttatt taccttgtga tttaccgggc
     5821 aatgccgcat attttatcaa aaccttaaag cccaacctgg ctgtttttgt gaaatacgac
     5881 ctgtggtatg gatacctcaa agtattgttt cagatgaata ttccggccat attggtgtca
     5941 gccattcagc acaaggcatt aacaggctgg tcaggagcat tgtataagaa gatatgttat
     6001 agattactgg acaaaatata tgtgcaggat gaattatcca tgaccaacat tcgggctgca
     6061 gtaaataggg aagtaatagt agccggcgat acacggattg acagcgtaat cagattttca
     6121 ggtgttaccg atcaccagct tgaccacgac atgaagcgat tcgtaggtga tgatagttgc
     6181 ctggtatgtg gcagtacatg gcctgccgac caggacctac tcattccctt gataaaagat
     6241 gaaattttca aaaactggaa ggctatcatt gctccgcatg aaatgaagaa agatgatttg
     6301 gagaatttaa aaaagcaatt tggagaaaag gccattttct acagcgaatt tgaaaggtac
     6361 gatcaaacac ataccgtact catcatagat tcgatcgggc atttgtcaaa aatatacaga
     6421 tatgcaacac tcgcatacat aggaggagga ttcggcaatg gcattcacaa tacattggag
     6481 ccggcagcat ttagcttacc agttatattc ggcccaaaat ttaaaaaatt cagcgaagcg
     6541 gtaaatctga caaaggacaa agcatttgcc ccgataaaca accaaaatga acttttcagt
     6601 acatttatat attataataa agtagaaaac aggaaaaatg ctgctgaagc tattcgagct
     6661 tatatgaata gtaataaagg agcatccgga ttgatattac ttgatataaa attgtattat
     6721 cttggcaatg aaaattaatc aaatgataca ggtatgagtt tttataaaaa tgtgctgggt
     6781 gaatttcgca acaaaaaagt actgatagca ggcgatatca tgacagacag gtatcttacc
     6841 ggtactgtta atcgtatttc acctgaagca ccagtacccg tcgtacaact tacacatgcc
     6901 gatgaccgct tgggtggcgc cggcaacgtg gcgttaaacg ttaaggctta tggagctata
     6961 cctatcttgc ttactgtcgt tggtgaggat gatgacggac ttaaactcct caagcagttg
     7021 aacagtctgg gaatagaaca caaatttatt tgcaagtcag tagaacgacg tactaccgta
     7081 aaaacccgta tcgtatcaca aaatcagcaa atgatgcgga ttgatgcaga agacacattc
     7141 gaactcacac accatgaaaa acaagttgtc atcgcacagt atgtcaatgc actaaaagaa
     7201 acaaaaccag atgtaatcat acttcaggat tacaacaaag gatttttcac tcctgaactt
     7261 atccaagtta ttacaagata tgcagctgat aataatattc cggtaacagt agatccgaag
     7321 aaggccaatt ttctagattt taaaggagtg actatcttca aacccaatct caaggaagta
     7381 aaagaggctc ttaacacgga gatcaaaccg gagataggtg acttgaaaaa tgcttcgggc
     7441 catatcaggg atatattagg caataagata accgtcatca ccttatcaga gaatgggttg
     7501 tttatagatg atggggttga tgcaaagatt tatcctacaa caccgcgtaa tatttcagat
     7561 gtgtgtgggg caggagacac ggtcatcagt acattaagtc taggtatagc atcagggctg
     7621 agtgcatacg agttggctgt tatggcgaat gtagcaggtg ggcaggtgtg cgagcgtccc
     7681 ggagtagtgc ctgttgatcc ggatcagcta attttagagt tgtcagagac tttatattca
     7741 gaaaaaacat gatttttttg acatttcttg attttttaac atttatataa aaaaaaaggc
     7801 ttatttttac cagcgattat taaaatgttt aaacccaatt taattatgaa attaaaattt
     7861 ttacaaacca gatttttttc tgcatttgta tttgttgtag ccagtatgtg gagcatgaca
     7921 gcttttgcac agccggctaa tgacttatgc tcaaatgcaa tcgaacttac tattgctgct
     7981 gatgaagcat ccgctgtgcc ggtagcagga acaactgtag gtactgttga cggtgctact
     8041 actcctggcc caaaagtttg ttcagcaaac ttttaccgtg atgatgtatg gtacaaggta
     8101 acaattcctt caggtgttgc agctgaagca gtaaccattg ctgttaacag aaatgttaca
     8161 ggtggtatga cagattgtgg tatggctatt tatgtgagca atgcatgtga taccagcaat
     8221 gctgcatact tgtgtcagaa ttttacagga aacggatctt ttagattcag taaaatctgt
     8281 acaggtgaag ataaggagct ccttataagg gtttggtcag gtgctggtac agctgctaac
     8341 tgggctcaag gccagggtgc atttgagata tctgcattct actctgatgg cgagcaggct
     8401 atttggggta ataacggaga aggttctttt aacggtggtc tgggtggctg gacaactact
     8461 ttggatgcaa aatgcccggg ttttgaactc tggagatggt caaatacaac ctactgtaca
     8521 aatggagcat tctcttcagg tggtggtacc atcacatcat tgacagtttg taacggagca
     8581 atgtgcttcg actctgacta ctatgataat aacggtcaat caacaggtat cggtactggt
     8641 ccatgtactg cacctcaaga gggttcactt gtttctccag ctattgacct tagcgtcttt
     8701 cctgacgtaa caggtgttaa cttggttttc tcacaggcaa caagacaatt ccagagtgaa
     8761 tactatatag aactaagcct tgatgaagga actacatgga acacaatcga aatcaatact
     8821 gcggaagaag atccggataa ttatgctgtt aacggtccgc atgtaaacaa caacagaaga
     8881 gttttcctac caggtgctgc cggaaaacca aatgttcagg ttagattccg ttatgtaggt
     8941 aactactact actggatagt agatgatgtg aaaatcacag aaagagaatc ttacaacctt
     9001 agagtagatc cattctatgc tattgcacct aacaagcagt ggcagagaga tcagttagag
     9061 tcattcggtg gtttggctga tatttctaac aatggttcga aaggaactac caatactgca
     9121 cttagccttg agatcaagga cgctgcaagt acaactatct ttacagacga gctaagctat
     9181 ggcactactg tagcagacag cacgtatgaa aatgtaccta tgactggtac cttcacacac
     9241 cctaatgatg atgtaactac ttatacaggt acttatgcac ttacttcaac ggagcctgac
     9301 tttgactata ctgacaattc aagaaatttc tcttggtcag taactgactc cattatggca
     9361 aaagacggtt ctacaactct tggtaatggt atcagagctg gtagcgatta taactatacc
     9421 tggggtaaca tttaccatat cgtaaacaac aagagcagtc ttaatggtaa gacttatcat
     9481 tgtaactatg ctggagttgg tatagctaat ccggatgacc tggcaggtgc taccatcttt
     9541 gtttacttgt acaaatggga taatgccaac ggtgatgatg taatacaagc agatgaaaga
     9601 actacagtgg gctttaacca gtatgacttc cctgtaggtg aggctgccaa tacgctttat
     9661 acactcccaa tatttgacta tgctactttt gaacatggtg ttgcactcga atcaaatacc
     9721 gattatgttt tggtagtaga atataccact cctcctgacc aactagaatt accttgtttt
     9781 atcaactcag atgcatcgat cgattacgga gctcagacag tgagatctac aactcctggt
     9841 tttggtcctg taagatacaa ccatgcactt gacgtaggta acacaggcga aatgactgca
     9901 aatacattca caggtggtac tacaccggtt atcaggatgt tcctaagcac tactactatc
     9961 agtacaaatg aagttaaact tgatgatgct aacaagattg ttgtagctcc taatccggtt
    10021 aaagatgtac ttaatgttaa aatcgacctt gtagaaaatg caaaagaagc tactatcgaa
    10081 gtagtggata tcaacggtaa gattcttaat acaagaacaa tcgaaaactt caagtcaact
    10141 actgagacgt ttgatgtttc aaaccttgtt aatggtaatt actttgttaa ctataagtca
    10201 aaggctggtg taagatcagt taagtttata gtggctaaat aattaactgt taatcagtaa
    10261 tcatacaaaa gccacagagc atgttctctg tggcttttct tattaaatac atgtaggatg
    10321 tacgcgaata tcgaatttga agctaagtta aagttctata attgtgctta aaatcaaggt
    10381 tctgagctac aagtacaaaa agattcggtt tacttttata tccggataac gactatttaa
    10441 gatttctgag actatcttcc aaaaatccga ttcttgacaa tccgtcattc atcttctgtc
    10501 tttctttatc taccactgca gccggtgcac cacttacaaa acgctcattt gaaagtttct
    10561 tttcgatcga agcaacgaat cctcgctggt actcgagttc tttctctatt ctttctcttt
    10621 ctgcttcgat atcaatctcg atatcaagta aaacgtaata tttatcggct ccacttacga
    10681 ttgaaataca tttttcgggt tcgttttcgg ttatttcaat actacttagg tttgccattt
    10741 ttttcagcat gtttttaatg ccttcgttgg ctaaccagtc ggtattttgt ggagaagggt
    10801 ttgcgaaaaa tggtagttgg tcggccatct ttacaccttt gctgttgcgc acctcacgta
    10861 ccttggttat tatttctttt acggcatcta tttgtctgat gaaatggaga tcaaatggct
    10921 ttaattcggg ctttttacct actatacaat tatcgccggg cactctgtct cttagctgat
    10981 gccagacttc ttcggtcaca aaaggcataa taggatgaag cattgtcatg agctgctcga
    11041 atatctgtac ggttttttca agggaatcag catctatcga ttcaccgaaa ggtggcttaa
    11101 tcatctcaag ataccaacca cagaaatcat cccaaatgaa actatacagg tcaactgtta
    11161 tctctgaaag tctgtagtct ttatagtttt cttcgtactt ttcgaatttc tgtgcgattt
    11221 tgttttcaaa ccattttact gcaatggtat taatttcctt ttggttaatg tcctttatat
    11281 cttcgcttac ctgccatcct tttataagcc gtagagcatt ccatatttta ttacaaaagt
    11341 tgaggccttg ttcgcataat ttactttcgt tgatgatttg cttagtttca ttgtcaaatg
    11401 gcgcgtcgaa gattatatca ttccctgcgg atgcactgga tagcaatcca aatcgtacac
    11461 catctgcacc gtatttggcc atcaactcaa gcgagtcagg cgaatttccg agcgatttgc
    11521 tcattttctg acgcttattg tcgcgtacca ttccggtaaa ataaacgtcc ttgaacggaa
    11581 aattaccttt tgcagccgac aattcggttc ctatcaagtc gcccgaccat tcgtagccag
    11641 ccattatcat tctggccacc cagaagaata tgatgtccca accggtgaca agtacttggg
    11701 tagggtagta gtatttgagt tcgtcctgtc tttcaaatcc gtcaaaaacc gatattggcc
    11761 ataaccacga agagaaccag gtatctacta cgtcttcatc ttgtatgaga tcagtaagtt
    11821 gaagtgaatg gttgtctagt ttttccttcg cctggctgag agcatcttct gcagttgttg
    11881 ctacgaatac atgttcacca atgtaccagg ccggtatctg ttgcccccac cacaattgtc
    11941 tggaaataca ccagtctttg atgttgtctt ctgcaagcca ggtacgtagc atattgatga
    12001 aggagtccgg aaaaaatctg atgttacctt gctctactga tgataaagcg gtattagcaa
    12061 attccttcat attgagaaac cattgaagtg taagacgagg ctccactata gcattcgatc
    12121 gttcagatct gccgacttta tttctgtagt cttcaatttt tgctatctga cccagtgcat
    12181 ctatatcttt aacgattgcc tttcttgctt caaaacggtc cattccgata taatgcccgg
    12241 cgatgtcggc tatctttccg tcggggttca atatatcaat ggtttcaaga ttgtgttgtt
    12301 tgccgatttc gtaatcattg atgtcatggg caggggtaac tttgagtgca cctgtaccaa
    12361 agtccatttc tacataacga tcgaaaataa tgggaactac tctgttaaca attggcacaa
    12421 tagctttttt gcctttcaaa tgtgcatatc gttcatcatc cggatttacg gctatggctg
    12481 tatcgcccag tatagtctcc ggtcgggtag tagcgatggt gatgtattcg tcggtgcctt
    12541 ctattctgta ccttacatga aacaacctcg actgctcttc agagtaaatg acctcttcgt
    12601 tagacaaaac tgtttgggcc tctgggtccc agttggtcat tcgtagtccc ctgtatattt
    12661 ttcctttttt atacagatca acaaacacac ctgttactgc attagacaga gaaggctcca
    12721 ttgtaaagcg ggtacggtcc cagtcacagg agcatccgag tttcttcagt tgttcgagaa
    12781 ttattccgcc atatttttct ttccattcga atgcatgacg cataaattct tcccgggtca
    12841 gatcactttt tttgatacct tgtttgcgga gcaattgtac aaccttggct tctgtggcaa
    12901 tcgaggcatg atcggtaccg ggcacccaac aggcattctt gccatctata cgagctttgc
    12961 gtatcaaaat atcctgaatg gtattgttga gtaaatggcc catgtgcagc acgccggtaa
    13021 tgttgggtgg tggcatcaca atggtatatg gctctctgtc atcaggcaca gaatggaaat
    13081 aattgtgttc catccagtgg gtgtaccatt tattttctat atcattgggc aaatatcgtg
    13141 ttgacaatgt catctgcggt gcttgtttgg tcagtattta aaaatataaa tcgtctttgg
    13201 tccgttcttt ggaattttta caaaaaaaag atttgaataa tcgtcctcat cgagttcggc
    13261 catattgctg cctgtaagta aattgattaa tgcaggtaca gaattggaat gccccgatat
    13321 gagaatgcgc ttatatggtg ttagctttgc ggccagttct gcctgatttg caggattgta
    13381 taactgccag ttgatgtttt ttgacagaca ataaggctta atggtattga ttgtacggat
    13441 aaaatctgaa gaataaacca tgtctgctac cggtgccttc ctaagtatct tgctcaatct
    13501 ttcagatctt gcctcacctg cctcagacaa ctcggcattt tttgaaaaat ctgcttt
//
LOCUS       DAOQQY010000044        13513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1533_length_13513_cov_6.870709, whole genome shotgun sequence.
ACCESSION   DAOQQY010000044 DAOQQY010000000
VERSION     DAOQQY010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13513
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1533_length_13513_cov_6.870709"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1864
                     /locus_tag="PLE23_03910"
     CDS             <1..1864
                     /locus_tag="PLE23_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HMX82442.1"
                     /translation="HLLNDVEALKIMLEEDYFETGITRIGAEQEMCLVYRDSLKPAKI
                     NMEIIEKMKDYPWLTTELAKFNLETNFDPKEFTGSCFRDLEAENHEYLNMIQEYLHPY
                     NASIILTGILPTLQKNDLDIENLTPKDRYYALMNSIKAQLIGQSFELRLNGIDELNIR
                     HDSPLLEACNTSYQVHLQVSPKDFVKMYNISQAITAPVLAIAANSPLVFGKRLWHENR
                     IALFQQALDTRTTQDHMRERSPRVNFGKGWLQDSILEIYKEDISRFRVLLGTDIEEES
                     LELIKNGKVPKLKALQVHNSTVYRWNRPCYGISANGKPHLRIENRVLPAGPTIQDQTA
                     NAAFWLGTMMAAGEQYSDITSQISYEDVRDNFMKASQFGIDSEFTWFKDKKVSACDLI
                     LEELLPMARQGLKSCKVDSGDIDKYLGIIEERAKKHMNGSRWILRAFTKLKSETTVDE
                     ALSVLTSCIISNQQKHIPVHNWPLPNLNDMEHYNPSQMQVNEFMETDLFTCQKDDPVE
                     LVADMMDWRKINYCPVEDTKGNLIGLITSRLILRYYAKRAKMKTEDAVLVRDIMIDTP
                     ITIGPNERIIDAVEKMKKHGIGSLPVVQGNELVGIITEMDFLRISARLIQNSGK"
     gene            2063..5413
                     /gene="ccsA"
                     /locus_tag="PLE23_03915"
     CDS             2063..5413
                     /gene="ccsA"
                     /locus_tag="PLE23_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMX82443.1"
                     /translation="MRHAATTGHFDIAKITTIQTNKILMNPLSKMLFSTRTMTVLLLL
                     YGISMAVATFVENDFDTPTAKTLIYNASWFELLMLWLVVLFIRNISVYRLTRREKWPV
                     LAFHLAFVFMFIGGAVTRYISFEGQMPIKEGQTTNEIISDLTYFKLHVSDGEKAMIYD
                     KYPYQMSYFNGKITSWPFKRSFSQDFIFDKKTITLKVLDYIPLAKDSVVLTESGKRMI
                     KLVSMGEQGRVNNYIAEGEIKSINGTLVGFNDTTEGIVRLVEKNGDIELTLPTDGEYM
                     SMQGQMMGMITDTTKFSQNSGTLLANQASMVNHRALYTVGNGSFIIPEKPFNGVMKYF
                     KGDKNNPSEKNLPGLIEVELSSGKDRDTVNLKGGQGILTLNNPVKLNGLDVSVGFGSK
                     MLHTSFFLRCDDFKLDHYPGSNNPSSYESKITVIDGEKEKQHRIYMNNVMDYRGYRFF
                     QASYFPDQSGTILSVNADRMGTNITYFGYFLLFAGMFFSLFWKDTHFWKLNESLKQMH
                     TMKSVLVIVAMCVSSIGYGQNGVHDSTHVHQMSEHEANPTAQFARPDELGPDKRINPI
                     HAEKFAHLLVQDFQGRIKPMNTHTLELLRKLYKKDHYKNAGLSLSSEQWFISMQVDPA
                     FWATEPIIKVGSKGGDALLKETKANDEGYTSYANLLDPETGAFILEDKVNASFSKRKA
                     DQTNYDKEVIDVTERFNIFSNVAYGYYTKVVPVKDDLNNTWHSWIYSSDHNSVEIDDT
                     AYVVLTNYFNSVKDGLKTGKWEKANENIDIMSKLQKTWGAKVVPADAKVNLEILYNHA
                     NAFFWLMIVYSMLGMCLVLLGFAEVLSTGTTYNKLIRVLTKVLLGFLVAALLVQAVTL
                     GIRWYLSGHAPWSNGYEAIVFISGIGVLAGLILYKNRNAFIPAAGALVAMIMMGFAHG
                     GSMLDPQITPLEPVLKSYWLMVHVGIITSSYGFFGLSAVLSVISLILYCVKPTDKIQH
                     SIKEMTIVNEMSLTIGIFALTIGTFLGGMWANESWGRYWSWDPKETWAFISVIFYAVV
                     LHLRLVPKLRGTLTFNIASLWAIWSVIFTYFGVNYYLSGLHSYAAGDPMPVPAWIYIS
                     ATAMLVLTLSAYLRKKLTDKAQNFGQSA"
     gene            complement(5502..7397)
                     /locus_tag="PLE23_03920"
     CDS             complement(5502..7397)
                     /locus_tag="PLE23_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMX82444.1"
                     /translation="MSDPIKHECGIAMIRLLKPISYYEEKYGTSFYGLKKLQLLMYKQ
                     RNRGQDGAGVATIKIGMKPGERYISRRRSNSPNYLRDLFDGIFSNFVNLPDEQLHNPD
                     WLKNNKPYIGELLMGHLRYGTHGNNSVENCHPFLRQNNWISRNLVLAGNFNMTNVEEL
                     FEELVELGQYPKEKTDTVTVMEKIGHFLDDEVQRVFDWFKSDGYSNTKINQLIVQHLD
                     LQRILTRASKKFDGGYVISGMIGHGDMFVMRDPNGIRPAFYYQDEEVVVAASERPAIQ
                     TVFDVPFASIKEIKPGHALIVRSNGKLDEQPFIQQSTATPCSFERIYFSRGTDRDIYT
                     ERKKLGELLAKPALEAVGYDFKHTVFSFIPNTAEVAFYGLIEGLEKELNNVKLDKITK
                     LGDKPNASKLKDILSMHVRAEKLIVKDDKSRTFIADEATRGNMVSHVYDVTYGLVENN
                     TDTLVLIDDSIVRGTTLRDSLISIAARLLPKKIIILSSAPQIRYPDCYGIDMSVMGDF
                     VAFSALVALLKENGQEHLLDETYEKCKSQQSLPKDQIKNYVSELYGLFSYEKITQKVT
                     DIITPKGIGPKIEIIFQTLDDLHTACPNHKGDWYFSGNYPTPGGNKVVNNAFINFMEK
                     SNVRAYY"
     gene            complement(7552..7624)
                     /locus_tag="PLE23_03925"
     tRNA            complement(7552..7624)
                     /locus_tag="PLE23_03925"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7589..7591),aa:Phe,seq:gaa)
     gene            complement(7786..8481)
                     /locus_tag="PLE23_03930"
     CDS             complement(7786..8481)
                     /locus_tag="PLE23_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82445.1"
                     /translation="MINVVVCQARFLLLLIIAGVSFLITSCHPDQEAKKQYLSSQEIQ
                     AQLKKIWHDDQIYRARAGQLRQENKGVRTPEEGELWAKQTILDSINMNLVEAIIEKVG
                     YPTKTMVGDSLKHVAALVIMHNPKRQSKYVNTIWSAARNGDILLTDAATLEDRVLMFA
                     GKNQKYGTQMKYDTISIDKKSGTVNTQLRIWPIENAKIVDSLRASIGLYPLSRQCEMM
                     NLDCSKVKGYTVK"
     gene            complement(8478..8711)
                     /locus_tag="PLE23_03935"
     CDS             complement(8478..8711)
                     /locus_tag="PLE23_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82446.1"
                     /translation="MEEQNKKITEKQEPNAQFANNKDRNICYSMLPPNLQENIMPSYD
                     QDENFRKLIKERVDRHKSNSGLLENILRKLKGK"
     gene            complement(8763..9326)
                     /locus_tag="PLE23_03940"
     CDS             complement(8763..9326)
                     /locus_tag="PLE23_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX82447.1"
                     /translation="MNNEKENIFEEELLSHIDALHNFAFYLTHNAQEADDLVQETYFK
                     AFKHINYFQDGTNSKAWLFKILKNNFINEYRKKNRGGVKVDFEDALRGVEGDIDIEIP
                     ENIEFDPELAGMILGDEVTNALNSLSVEYRSLIVLCDLEDFSYEEISKILGIPLGTVR
                     SRLHRARAMIKIKLYDYAKKIGISTKK"
     gene            complement(9487..10128)
                     /locus_tag="PLE23_03945"
     CDS             complement(9487..10128)
                     /locus_tag="PLE23_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMX82448.1"
                     /translation="MEVQDITDHFFVKRPEIPAYSAYQKILERNGLFALLEPETSIEA
                     EFITRPEVIEGLMWGLPRYGHPEGKVAYHIKEVFNNINNLRNEDADTRTMLRIIALIH
                     DTFKYQEDRNVPRDWTKHHGVLARKYLENQNLSFELCEITEMHDEAFYAWRAAVVEKN
                     QELSDSRLKRLENRIPNHLNEYFLFFLCDTLTGDKLRTPVKWLFEKAKTNGWF"
     gene            10263..11393
                     /locus_tag="PLE23_03950"
     CDS             10263..11393
                     /locus_tag="PLE23_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMX82449.1"
                     /translation="MLKNIIPFIISGILGGIIVLGGTRILQKEKPAEMISKPAANVAY
                     TGSVGSIPASFAYAAEKTMPSVVHIRAKESQQTANKRMYRSPFGEDFFWGYGAPRAQE
                     GTGSGVIISPDGYIVTNNHVVDFADYIEVTLHDDRKFKATVVGRDPKSDLAVIKIDED
                     NLPAIQFGDSDNIKVGEWVLAVGNPFNLNSTVTAGIISAKHRKLDVIPEKDALEAFIQ
                     TDAAVNPGNSGGALVDPEGKLVGLNSAIASPTGSYAGYSFAIPVNLLRRIVDDLIQYG
                     NVKKGALGVLVNDIDEKLISEHRLKVKEGMFVNDVVSGGSAQSAGIQPKDVIVAIEGK
                     KIQNFDDLRENIALAKVGQVINITINRNGQNKVMPVKLKSGG"
     gene            complement(11468..>13513)
                     /gene="recG"
                     /locus_tag="PLE23_03955"
     CDS             complement(11468..>13513)
                     /gene="recG"
                     /locus_tag="PLE23_03955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMX82450.1"
                     /translation="KADLLAKELNIRSVEDLLNHFPFRYIDKTKFQKISELTGDEDYV
                     QIKGQVVQLQTLGAGRSKRLVAQFTDSSGQIELIWFQGIKWIEDSLQLRTEYIVFGRL
                     TEFNRKFSIAHPEMELMVGHPAVSFLAPVYPSTDKLNKKGLDTKSQRRIFRQLTEKLL
                     PADLPEILPLYIVEKLKLLSRFQAYQFIHFPPDSAHLHAAQKRLKFEELLCMQLKILQ
                     YKNRKQSLIKGYIFEKVGDLFNGFYSKYLPFELTNAQKKVIREIRKDMGSGKQMNRLL
                     QGDVGSGKTIVALLSMLIALDNGFQACLMAPTEILAQQHFQSIEEFIKPLNIGIGYLS
                     GSVKGKTRNQVLTALKDGSIKIIIGTHALLEDWVEFQKIGLAIIDEQHRFGVKQRAAL
                     WSKSTVPPHILVMTATPIPRTLAMTLYGDLDVSVIDELPPGRKEIKTLHFNENKRLRL
                     LGFIREEINKGRQIYIVYPLIEESEKLDLLDLENGYQALLREFPMPEYHISVVHGKMK
                     PEDKEYEMSRFIKKQTQIMVATTVIEVGVNVPNASVMIIEHAERFGLSQLHQLRGRVG
                     RGADQSYCILMTSFKLSKDSRERIKTMCETNNGFVIAEADLRLRGPGDIEGTRQSGLF
                     GLNIADLAKDAAILTTAREIALRILDSDPEMNEPQHTAIKRHIDHRYRNLQDWSSIS"
BASE COUNT         3849 a         2628 c         2860 g         4176 t
ORIGIN      
        1 acatttactc aatgatgtag aagcacttaa gataatgctt gaagaagatt actttgagac
       61 cggaataacg cgaatagggg ccgaacagga gatgtgctta gtttatagag attcgctcaa
      121 gccagcgaag atcaatatgg aaatcatcga aaagatgaag gattacccat ggcttacaac
      181 cgaacttgct aagtttaatc ttgagaccaa ttttgaccca aaagagttta ccggtagctg
      241 ttttcgtgac ttagaggctg aaaatcatga gtatctcaat atgatacaag aatatctgca
      301 tccctacaat gcgagtatta tacttacggg tatattgccg acattgcaaa agaatgacct
      361 cgacatagaa aacctgacac ccaaagacag atattatgcc ctgatgaatt ccataaaagc
      421 ccagcttata ggtcagtcat tcgagctaag gctcaatggt attgatgagc ttaacatccg
      481 tcacgattca ccactccttg aagcctgcaa cacctcttac caggtacacc tgcaggtatc
      541 acctaaggat tttgtaaaga tgtacaatat ttcgcaggct attacggcac ctgtgcttgc
      601 cattgcagcc aatagcccgc tcgtatttgg taagagactt tggcatgaga accgtatagc
      661 cttgtttcag caggcacttg ataccagaac cacacaagat cacatgcgcg aaagaagtcc
      721 gcgtgtcaat ttcggcaaag gctggctaca agattcgatt cttgaaattt acaaagaaga
      781 tatatcacgg ttcagggtat tattaggcac tgatatagaa gaggagtcac tcgagctcat
      841 caaaaacggc aaagtgccaa agctaaaagc gctacaggta cataattcga cggtgtaccg
      901 gtggaaccga ccatgctatg gtatttcggc aaatggaaaa ccacatcttc gcatcgaaaa
      961 cagagttttg ccggcaggcc ctacaataca agaccagacg gcgaacgctg cattctggtt
     1021 aggcacgatg atggcagcag gagagcagta ttccgacatt acttctcaga tatcgtatga
     1081 agatgtcaga gacaatttta tgaaggcttc gcaatttggg attgactccg agtttacatg
     1141 gtttaaggat aagaaggtgt cggcatgtga tttgattctt gaagaattgc tacctatggc
     1201 acgacaaggc ctgaagtcct gcaaagtaga tagtggtgac attgacaagt accttggcat
     1261 catcgaagag cgcgctaaaa agcacatgaa cggttcaagg tggatactga gagcatttac
     1321 caaactcaag tctgagacca ccgttgacga agctttgtcg gtactcacat catgtatcat
     1381 ctccaaccaa caaaaacaca taccggtaca taactggcca ttacccaacc ttaacgatat
     1441 ggaacattac aacccgtcac agatgcaagt aaatgaattt atggaaaccg acctgtttac
     1501 ctgtcagaaa gacgacccgg tagagctggt agcagacatg atggactggc gaaagatcaa
     1561 ttattgcccg gttgaagaca ccaaggggaa tcttattggt ctgatcacct caagactcat
     1621 acttcgctat tatgcaaaga gggcaaagat gaaaacagaa gatgccgtac tggtacgtga
     1681 tatcatgatt gatacaccca taaccattgg cccgaacgaa cggataattg atgctgtcga
     1741 aaaaatgaaa aaacatggca ttggcagttt gccggtagta cagggtaatg aacttgtggg
     1801 tatcattaca gagatggatt ttctgcgaat ctctgctagg ttgattcaga attcaggtaa
     1861 ataagcagct tcataatagg tgataattaa ctaatattct tggatcttgt tttttattca
     1921 ttcaagttaa gtatttaact cacattatag ctttatcaag gttataattt cctgatttaa
     1981 tcttactttc gcattgaaat aaaaagtacg aaccttttcc ggtttaaatg attatatagc
     2041 atgaaatagt tcggataaaa ttttgcgcca tgctgctaca accggacatt ttgatatagc
     2101 aaaaattaca acaatccaga ccaataaaat actaatgaac ccattgagta aaatgttatt
     2161 ttcaacacgt accatgaccg tacttttgct attatacggt atatcgatgg ctgtggcaac
     2221 attcgttgag aatgactttg atacacctac tgccaaaaca ttaatataca atgcatcatg
     2281 gttcgaatta ctgatgcttt ggcttgtggt tttgtttatc cgaaatattt ctgtctatag
     2341 acttacacga cgtgaaaaat ggccggtttt ggcatttcac ttggcgtttg tatttatgtt
     2401 tattggcggt gcagttacac gctatataag ttttgaaggc cagatgccta ttaaggaagg
     2461 tcagaccacc aacgaaatca tcagcgacct tacttatttt aagctacatg tgagtgatgg
     2521 cgaaaaagct atgatttatg ataaatatcc ttaccagatg tcttatttca atggaaaaat
     2581 tacgagttgg ccattcaaaa gatcattctc tcaggatttt atttttgaca aaaaaaccat
     2641 aacacttaaa gtgcttgatt acattccgct ggcaaaagat tctgtagtac taacggagtc
     2701 cggcaaaaga atgataaaac tggtatcgat gggagagcaa ggaagggtaa ataactatat
     2761 tgccgaaggc gaaataaaaa gcattaatgg cacattggta ggcttcaatg atactacaga
     2821 aggtatagtc cgactggtag aaaagaatgg tgatatagaa ttaacacttc ctactgatgg
     2881 agagtacatg tctatgcagg gtcaaatgat gggaatgatt actgatacta ctaagttttc
     2941 acaaaattca ggtacactat tggccaatca agcttctatg gttaatcatc gtgcacttta
     3001 tacagtaggt aacggcagtt ttattatacc tgaaaaacca ttcaatggtg tcatgaaata
     3061 tttcaaggga gataagaata acccctctga gaagaatctt ccggggctca tcgaagtaga
     3121 gcttagttcg ggaaaggaca gggatacagt aaacctaaaa ggcggacaag gaatactaac
     3181 gcttaataat ccggtaaagt taaatgggct ggatgtgtca gttgggtttg ggtcgaaaat
     3241 gctgcacaca tcattctttt tacgttgcga cgactttaaa ctcgatcatt atcccggctc
     3301 aaataaccct tcgtcgtatg aaagcaaaat aactgtgata gacggagaaa aagagaagca
     3361 gcataggata tatatgaaca acgtgatgga ttaccgaggt tacagatttt ttcaggccag
     3421 ttatttccct gatcagtccg gtactatatt atcagttaat gcagaccgta tgggtaccaa
     3481 tattacctat ttcggatact ttttattgtt tgcgggtatg tttttcagtt tattctggaa
     3541 agatacccat ttttggaagc taaatgaaag tctgaagcag atgcatacca tgaaaagtgt
     3601 tttagttata gttgctatgt gtgtatcctc aattggttat ggacaaaatg gagtgcatga
     3661 ttcaacgcat gtccatcaaa tgtcggaaca tgaggcaaac ccgacagctc agtttgcccg
     3721 gccggatgaa cttgggccgg ataaaagaat aaatccgata catgctgaaa agtttgcaca
     3781 tcttctggtt caggatttcc agggaaggat caaaccaatg aacacacaca cattggagct
     3841 tttgcgtaag ctatacaaaa aagaccatta taagaatgca gggctgtcgc tcagttccga
     3901 acaatggttt atttctatgc aggtagatcc tgctttttgg gctacagaac caattatcaa
     3961 agtaggttcg aaaggtggag atgctctgtt gaaagaaaca aaagcaaatg atgaaggcta
     4021 tacatcttat gccaatctgc tcgaccctga gacaggagca tttatacttg aagataaagt
     4081 gaatgcatcc ttcagtaaga ggaaagctga ccagacgaat tatgataaag aagtcataga
     4141 tgttacagaa cgcttcaata tattcagcaa tgtggcttat ggctattata caaaagttgt
     4201 acctgttaag gatgatctga ataacacttg gcatagttgg atctatagct cagaccataa
     4261 ttcggtagag atagatgaca cagcttatgt tgtacttacc aattatttca actccgttaa
     4321 agatggatta aaaactggca agtgggaaaa agcaaatgag aacatcgata tcatgagcaa
     4381 acttcagaag acttggggcg ctaaggtggt tccggcagat gccaaagtaa atcttgaaat
     4441 actctacaat cacgccaatg cgttcttctg gctgatgata gtgtatagca tgctgggaat
     4501 gtgtcttgtt ttattgggct ttgcagaagt gttgtcaacc ggcactactt ataataaact
     4561 tatacgcgtt cttacaaaag tattgctcgg atttttggta gcagctttat tagtacaagc
     4621 tgtgacactg ggtataaggt ggtatttgtc tggtcatgca ccttggagta acggatatga
     4681 agcaattgta tttatctcgg gcatcggggt gttggcaggg ttaatcttgt acaaaaaccg
     4741 caacgcattc attcctgcag caggcgcact agttgctatg atcatgatgg gtttcgcaca
     4801 cggtggctcc atgctcgatc cgcaaataac accgctcgaa cccgtgctta aatcgtactg
     4861 gctgatggtt cacgtgggta ttattacttc aagttacggc ttttttggtt tgtcggctgt
     4921 attgagtgtg atttcgctga ttctttattg tgtaaagccc acagataaaa tccagcattc
     4981 gataaaggag atgaccatcg taaacgagat gtcgctcacc attggtatat ttgctttgac
     5041 aattggtaca ttcctgggtg gcatgtgggc taatgaaagt tggggacgat actggagctg
     5101 ggaccccaaa gagacctggg cattcatttc agtaatattt tatgctgttg tattgcactt
     5161 aaggcttgtg ccgaagctac gaggaaccct tacattcaat atagccagtt tgtgggcaat
     5221 atggtcggta atattcacct attttggggt caattattat ttgtccgggc ttcattccta
     5281 tgcagctggt gatccgatgc ctgtacctgc ctggatttac atttcagcta cagcaatgtt
     5341 ggtgttgaca ctgtccgcat acctgaggaa gaagttgaca gataaagcac aaaactttgg
     5401 tcagtctgcc tgatagaata ccagtacctg ctactgtaat gtattgttgc taatcattcc
     5461 tatggtaagg aagtgcaact ctgcaaaatg tagatttcac gttaataata agcccttaca
     5521 ttcgattttt ccataaagtt gatgaaggca ttattcacca ctttgttacc acccggtgtc
     5581 gggtaattac ctgagaagta ccagtcgccc ttgtgattag ggcaggcagt atgcaggtca
     5641 tcaagcgtct ggaagataat ttctattttt ggacctattc ctttcggagt aatgatatcg
     5701 gttacttttt gagtaatctt ttcgtagctg aataatccgt acagttctga gacgtagttt
     5761 ttgatttgat ctttaggcaa tgattgttgc gacttacatt tttcgtatgt ttcgtcgagc
     5821 aaatgttcct gcccattttc tttcagaagt gctaccagtg cagaaaatgc tacaaagtca
     5881 cccatgacag acatgtctat tccataacag tcaggatacc ttatttgcgg agcggacgac
     5941 aagatgatga ttttcttagg tagtaaccgt gcagcgatac ttataagaga atcgcgaagg
     6001 gttgttcctc taacgataga gtcatcaatg agtactaatg tgtctgtatt attttcaacc
     6061 agcccataag tgacatcata gacatgactt accatgttgc cacgtgttgc ttcatcagct
     6121 ataaatgtac gagatttgtc gtctttgacg atgagttttt ctgccctcac atgcatactc
     6181 agtatatctt tgagcttgga ggcattaggc ttgtcaccga gttttgttat tttgtctagc
     6241 ttgacattat ttagttcttt ttcgagtcct tcaatcaagc cataaaatgc tacttcagcc
     6301 gtattcggga tgaatgaaaa taccgtgtgc ttaaaatcat aaccgaccgc ttcgagtgca
     6361 ggtttggcta ataattcgcc tagttttttt cgttcggtat atatatcgcg gtcagtacct
     6421 cttgagaaat atattctttc gaaggagcaa ggcgtagcgg tactctgttg aataaacggt
     6481 tgctcatcca gttttccatt acttcttact atgagtgcgt gtccgggttt tatttctttg
     6541 atgcttgcga acggtacatc aaatacagtt tgaatggccg gacgttcgga agcagctact
     6601 actacctctt catcctgata ataaaatgca ggtctgatgc cgttggggtc acgcattaca
     6661 aacatatcgc catggcctat catgccactt atgacataac cgccatcaaa ctttttggat
     6721 gcacgggtga gtatgcgttg taaatccaaa tgctggacta taagctgatt gattttggta
     6781 ttggagtaac cgtcagactt gaaccagtcg aacactcgct gcacttcatc atcgagaaaa
     6841 tgaccaatct tttccattac agtaaccgtg tcggtttttt ctttaggata ttgaccgagt
     6901 tcaacgagtt cttcgaaaag ttcttctacg ttggtcatat tgaaattgcc ggccagcacg
     6961 agatttcttg atatccagtt attttgcctt aaaaatggat ggcagttttc gacactattg
     7021 ttgccgtgtg tgccatatcg caggtgaccc atgagcagtt cgccaatgta tggtttgttg
     7081 tttttgagcc agtctgggtt atgtagttgt tcgtccggta gattaacaaa gttggagaat
     7141 ataccatcga ataggtcacg caggtaatta ggagaatttg agcgtcttct ggatatgtac
     7201 ctttcgccgg gtttcatacc aattttgatg gtagcgacac ctgcaccgtc ttgtccgcgg
     7261 ttgcgttgtt tgtacattag caactgaagc tttttcagac catagaatga agtaccgtat
     7321 ttttcttcat aataagagat aggtttgagt aaccgtatca ttgcaatacc gcattcatgc
     7381 ttaattgggt cgctcataga cgaattttaa tgtgtgatta tttgaaggga tgcacaaagt
     7441 tacgttcttt ttcagagaac ttatttcgcg aacatgaaaa atatattttc ttaaaaaatt
     7501 ttaacacatc aataaaatga aaaggcccga gcgaaatcac tcaggcctat gtggtacctc
     7561 caggaatcga accagggaca catggatttt cagtccattg ctctaccaac tgagctaagg
     7621 caccatcttg cttgttggaa gcgggggcaa aggtaaaagt attttttgat atttacaata
     7681 gtatctcgaa aagtattttt ttaagagaaa tattttttgt aaaaataagc aatagacaaa
     7741 aaggcaagag ttgtgttatt tgaagcagaa ggggagtatt tctacttatt tgacagtata
     7801 acctttgacc ttgctacaat caagattcat catttcgcat tggcggctta aaggatacag
     7861 accgatggat gccctgaggc tgtcaacgat ttttgcattt tctatcggcc agattctgag
     7921 ttgcgtattg acggtaccgc tttttttatc gatactgatg gtgtcatact tcatttgtgt
     7981 accatacttc tggtttttgc cggcaaacat caatactcgg tcttcaagag tagctgcgtc
     8041 ggtcaataat atatcaccat tgcgtgctgc gctccagata gtatttacat atttcgactg
     8101 ccgttttgga ttgtgcatga taacaagagc tgctacgtgt ttgagcgagt cacctaccat
     8161 agtttttgta gggtagccta ctttttctat gatagcctcc acgagattca tgttgatgga
     8221 gtcgagtatt gtttgttttg cccagagttc accttcttca ggtgttctga cgcccttgtt
     8281 ttcttggcga agttgtcctg cgcgggcacg gtaaatctgg tcgtcatgcc aaattttttt
     8341 tagctgtgcc tgtatttcct gagaagaaag atattgcttc tttgcttcct gatccggatg
     8401 acatgatgta attaaaaatg agactcccgc tatgataagc agtaatagaa atcgtgcttg
     8461 acacacaacg acgttaatca tttacctttt agttttcgga gaatgttttc tagcaaacca
     8521 gaattgctct tgtgtcggtc aactctttct ttgataagct tcctgaaatt ttcatcttgg
     8581 tcgtaagatg gcattatgtt ttcttgtaaa ttgggaggca acattgagta acaaatattt
     8641 ctgtccttat tatttgcgaa ttgtgcattt ggttcctgtt tttctgttat ttttttattt
     8701 tgctcttcca ttgttgggtc aatttttaca tttccaaata ttaatttttg aatttcatat
     8761 aatcattttt ttgtagaaat gcctattttc ttagcataat catagagttt gatttttatc
     8821 attgcacgag cgcggtgcaa tcttgaacgt actgtgccga gcggtattcc cagaattttt
     8881 gaaatttctt cgtacgagaa atcttcaaga tcacacaata caatcaaaga acggtactct
     8941 acagaaagac tattcagagc attggttact tcatcaccca atatcattcc tgccaactcc
     9001 gggtcaaact caatgttttc aggtatttct atatcaatat caccctcgac tcccctaaga
     9061 gcatcttcaa aatcaacttt aacgccacct ctgtttttct tgcgatattc gttgatgaag
     9121 ttgtttttca atatcttaaa caaccatgcc ttcgaattag ttccatcctg gaagtaattg
     9181 atgtgtttga atgctttgaa gtaagtttct tgtaccagat catctgcctc ttgtgcattg
     9241 tgtgtcagat aaaaggcaaa gttgtgtagt gcatctatgt ggctgagcaa ctcttcttca
     9301 aaaatatttt ctttttcatt gttcatgccc caaaagtagc attactactc aatattttta
     9361 acatttaaac tccccaattt tgtaacatac ctcgatttta tcacacaact tggagacaaa
     9421 atctacattt tgattgttga ataatatgct ataataactt tatgcgcaat atttgtccaa
     9481 ccagtattaa aaccacccgt tcgtctttgc cttttcgaat agccacttga caggtgttcg
     9541 gagtttgtcg ccggtgagtg tgtcgcacag aaaaaacaga aagtattcgt tcaaatggtt
     9601 aggaatacga ttttccagtc tttttaaccg agaatctgag agctcctgat ttttttcaac
     9661 cactgctgcc cgccaagcgt agaatgcttc atcgtgcatt tcagttattt cgcataattc
     9721 gaaactcagg ttttggtttt cgagatattt gcgtgctagt acgccatgat gcttagtcca
     9781 atcacgtggt acatttctgt cttcctgata tttaaatgtg tcgtggatga gtgcgataat
     9841 tctcaacatt gtacgtgtgt ctgcatcctc atttctaaga ttgttgatgt tattaaatac
     9901 ttcctttatg tggtaagcta ccttaccctc aggatgacca taccgaggca gtccccacat
     9961 aagtccttct attacctccg gtcttgttat aaattcggct tcgatggaag tttccggttc
    10021 gagcaaggca aacagaccgt tgcgttcaag tattttctga tacgctgaat atgccggtat
    10081 ttcgggtctt tttacaaaaa aatgatctgt tatatcttgt acctccatag gcaagcagag
    10141 cgataatgtt gttattgtta aaagatagtt aaacaaatta attcataaaa tctaattatg
    10201 cttctattcg ttaactttac atacacattt tatacatcaa acatacaaaa tctcaattaa
    10261 ttatgttaaa aaatatcatt ccatttatca tttcaggtat ccttggtggt atcatagtat
    10321 taggtggcac aaggatttta caaaaggaaa agccggcaga aatgatcagt aaacctgccg
    10381 ctaatgttgc ttatacgggt agtgtaggct ctattcctgc aagtttcgct tatgcagcag
    10441 aaaaaactat gccgagtgtg gtccacatcc gggcaaagga aagtcagcag acggccaaca
    10501 aacgcatgta caggagtccg tttggtgagg atttcttctg gggctatggc gcaccaagag
    10561 ctcaggaagg taccggctcg ggtgtcatca tctctcctga tggatatatc gttacaaaca
    10621 accatgtcgt tgattttgca gattatattg aagtgacgct gcacgacgac agaaaattta
    10681 aagcaacggt cgtagggcgc gatccgaagt ctgaccttgc agtgataaaa atcgatgaag
    10741 acaatttgcc tgccatccag tttggcgatt cggataatat taaagtaggc gaatgggtgc
    10801 tggcagtggg caatcctttt aatctcaact ctactgtaac ggcaggaatc atttcagcga
    10861 aacacaggaa actagatgta atacccgaaa aagacgcttt ggaggcattt atacaaacag
    10921 atgcggctgt caatccgggc aattcgggtg gtgcgcttgt tgatcctgaa ggtaaactcg
    10981 tagggctaaa ttcagccata gcttcaccta caggttctta tgctggctat tcttttgcta
    11041 tacctgtcaa tctactcaga cgaatagtag atgaccttat ccagtatgga aatgtaaaga
    11101 aaggtgcact tggtgtattg gtcaatgata ttgatgaaaa acttatcagc gagcacagat
    11161 tgaaagtaaa ggaaggtatg ttcgtgaatg atgttgtgtc aggcggatca gcacaatcag
    11221 ccggtattca gcccaaggat gtgatagttg ccatcgaagg taaaaaaata cagaatttcg
    11281 atgatttgcg tgagaatatt gcacttgcaa aagtcggtca ggtaataaac ataaccatca
    11341 accgcaatgg gcagaacaag gttatgccag taaaattaaa atccggcggt taagtcaaaa
    11401 atctttataa cctcttatct atattcgttt ctaggcgaca gttatcatat ctgtcgcttt
    11461 ttttgtttta tgaaatgctg ctccagtctt gcagatttcg atatctgtgg tcaatgtgtc
    11521 gttttatggc tgtgtgctga ggttcgttca tttcaggatc tgaatcgagt atgcgtagtg
    11581 ctatctcacg tgcagttgtt aatatggccg catcttttgc cagatcggca atattaagcc
    11641 caaataaacc actctgtctc gtaccttcta tatctcccgg gccacgaagt cgaaggtcag
    11701 cttctgctat tacaaaacca ttgttggttt cacacattgt ctttatacgt tcgcgactgt
    11761 ctttggataa tttgaaagaa gtcattagaa tacagtacga ttggtcggca ccacgcccca
    11821 cacgcccacg taattgatgc agttgcgaca atccgaatcg ctcggcgtgt tctataatca
    11881 ttactgaagc attcggtaca ttgacaccta cttctatgac ggtggtagct accattatct
    11941 gggtctgttt tttgatgaaa cgcgacattt cgtattcttt gtcttcaggt ttcatcttac
    12001 catgtactac agatatatgg tattcgggca tcgggaattc tcttagcaat gcctggtagc
    12061 cgttttcgag atccagcagg tcgagttttt ctgattcttc gatgagcgga tacactatat
    12121 atatctgtct gcctttgttg atctcttcac gtatgaatcc tagcaggcgt agtcttttat
    12181 tttcgttgaa atgaagtgtc tttatttctt tacggccggg tggcaattca tcaatgacag
    12241 aaacatccag atcgccgtat agcgtcatgg ctaatgttct tggtataggt gtggcggtca
    12301 tcaccagtat gtgaggcggc acagtgcttt tagaccaaag tgctgctctt tgtttgacac
    12361 caaagcggtg ttgttcatcg atgatagcga ggccgatttt ctgaaactct acccagtcct
    12421 cgagcagtgc atgagtgcca atgattatct taatagaacc atcttttagt gctgtcagta
    12481 cctgattacg cgtttttcct ttgacagagc cagagagata gccaataccg atattgagtg
    12541 gtttaataaa ttcttcaata gactggaaat gctgttgtgc aagtatttcg gtcggtgcca
    12601 tcagacacgc ctgaaacccg ttgtcgagtg ctatgagcat tgacagaagt gctacaatgg
    12661 tcttgccact accgacatca ccctgtagta gtctgttcat ttgttttccg ctgcccatgt
    12721 ctttgcgtat ttctctaatc actttttttt gagcattggt cagctcaaac ggcaggtatt
    12781 ttgagtaaaa tccattaaac aaatcgccaa ctttttcgaa aatatatcct ttaatcaacg
    12841 attgtttgcg atttttatac tgaagaatct ttaactgcat acagagcaat tcttcgaatt
    12901 tcagcctttt ctgggctgca tgaaggtgtg cggagtctgg tggaaaatga ataaactgat
    12961 aagcctgaaa tcttgacaag agcttcagct tttcaacgat gtacagtggc aatatctcgg
    13021 gtaggtctgc aggaagcagt ttttctgtca attgtctgaa tatacggcgt tgcgattttg
    13081 tgtcaaggcc ttttttgttc agtttgtcgg ttgaaggata aaccggagcc aaaaatgaca
    13141 cagcaggatg cccgaccata agttccattt cgggatgtgc aatactgaat tttctgttaa
    13201 attctgtaag cctgccaaac acgatatatt cagttcttaa ctgtaaggaa tcttcgatcc
    13261 actttattcc ctgaaaccag ataagctcaa tctggccact tgagtctgta aattgtgcaa
    13321 ccagcctttt tgatctaccg gcgccgagtg tttgtaactg taccacctgg cctttgattt
    13381 gtacatagtc ctcatcacct gtcagctcag atattttctg aaatttggtt ttgtcgatat
    13441 agcgaaaagg aaaatgattg agcaagtctt caacagatcg gatatttaac tctttggcaa
    13501 gcaaatcggc ttt
//
LOCUS       DAOQQY010000045        13501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1537_length_13501_cov_5.787074, whole genome shotgun sequence.
ACCESSION   DAOQQY010000045 DAOQQY010000000
VERSION     DAOQQY010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13501
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_13501_cov_5.787074"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="PLE23_03960"
     CDS             <1..1376
                     /locus_tag="PLE23_03960"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMX82451.1"
                     /translation="MKTIIEPFRIKSVEPIRFNTAEQRAVLLRKAHYNPFYLHSDDVM
                     IDLLTDSGTSAMSSDQWAGIMHGDESYAGSPSFFRFEKTIRDITGMPFIIPTHQGRAA
                     EKILFSIMGGEGKYVLSNTLFDTTRGNIEFSGAVGIDLVCEEGKHPTVAAPFKGNMDT
                     AALIKEIERVGAGNVSVVVMTVTNNSGGGQPVSMHNIREVSSICKANGIPFFIDACRF
                     AENSYFIKKREAGYESKSVEEIAQEMFSYADGCTISAKKDAFANIGGFLAVHNEELAA
                     KCRNLLIITEGFPTYGGLAGRDLEAIAIGLREVLEEDYLQYRIRSMEYLTEKLVAAGV
                     PVMQPAGGHAVYLDAKQFMDHIPVNQYPGQVLACELYVQGGIRACEIGSLMFGKYDID
                     GKLIPAQLELVRLAIPRRVYTQSHIDYVAEVIIEVFANRHAQKGMRIIEEPPMMRHFT
                     VKMEKIG"
     gene            1454..1561
                     /locus_tag="PLE23_03965"
     CDS             1454..1561
                     /locus_tag="PLE23_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HMX82452.1"
                     /translation="MGKGDKKSKKGKIILGSNGNSRPKPNKVRKAKAQA"
     gene            complement(1639..1848)
                     /locus_tag="PLE23_03970"
     CDS             complement(1639..1848)
                     /locus_tag="PLE23_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82453.1"
                     /translation="MISIKNGFPDANSLLFACLLIFNYISVTKMWRIKNLPHFGKWLN
                     VCLIVPDAYLIGTDVELHIVQDSIS"
     gene            1882..2676
                     /locus_tag="PLE23_03975"
     CDS             1882..2676
                     /locus_tag="PLE23_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82454.1"
                     /translation="MESVLKSFLQFLQNATIGQWMIVVAGLPLIGYMIFMIFAFGWKS
                     RPVKGPDRWYEYVMLSYLLSFLWMWMISARVQDRANFLSFLPVNLILWPGPVLFHFIK
                     SKLYRNFRFRPRDLKHFIFPLAHLSFYFFAFALPPMRKYNLFLHEYHAIYKPFEDFSF
                     GVIMFLYCYFAYRFIKHEQWSINESTPKAEIVKISWLQRFFKVFFLSSFVHLAYGLFS
                     MANAFIFRIVFENNYLELLHIFVFMICMGWLGFHAVLMKALPKSNI"
     gene            2780..4717
                     /locus_tag="PLE23_03980"
     CDS             2780..4717
                     /locus_tag="PLE23_03980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMX82455.1"
                     /translation="MAKQVAKPKPIVKPAPVYKKFTYESGFFKKYWQQIAVLTALAVG
                     LYIYSVTFDYVLDDQIVMTKNEYVKKGVAGIGDILKTESMQGYFGEQKDLVVGARYRP
                     LSIVTFAIEYQMWGLNPVMSHLMNILLYALTGIFLYRILLMLFPKKDEQNSFFTIPFV
                     ASLLFVTHPIHSEVVANVKGRDEIMTLLGALGSVYYTLKYLSNRKSLYLVLSGIMFFL
                     GLMSKENAITFLAVIPATVYFFTNTEGSVNFKTVVPLLIATVVYLLIRYSVIGYFLSS
                     GQKVTDLMNNPFAQMTGSEKMATIFYTLLMYLKLLIFPHPLTHDYYPYAIPKLNWSSI
                     WAILSLLIHIGLAVFAIKNFKKKSLLSYCVIFYLATLSIVSNIVFPVGTFMNERFVYI
                     SSIAMCLAGAWLFVNYIPAIQNGRLKSLGLALTALFALAFCAKTIARVPAWKNKLSLN
                     VAAIKYSGNSARANCFMGTALFEEYQVEKDEAKRKAMLADINKYIDRSLEIYPDYYSA
                     LQMYSGVVGANYAQDGNLDKALDAFYKILSRRTTITYVDQFLDYLGNSPTVDQKKMQD
                     FYFKLGYDLFYKQKKYYNNAAKYLDKALKYNDLDPKANFAMSLVMSDIGDYEKADQFK
                     RVALTSDPGIITFFEQDIMRK"
     gene            4874..5971
                     /gene="dprA"
                     /locus_tag="PLE23_03985"
     CDS             4874..5971
                     /gene="dprA"
                     /locus_tag="PLE23_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HMX82456.1"
                     /translation="MNQEELFYRIAITMLPKVGPVTARNLISYCGGTREVFETSTKAL
                     MKIPGVGAAMSKSILQHDSFAPAEQELAFIDKQNIKPIFYLDDDYPKRLQNVYDAPVL
                     LYFRGDATLNAARTVGIVGTRTPTPHGIANCEALVEGLAKYNVQIISGLAHGIDGTSH
                     RAAVAAGISNIGVVAHGLDHIYPAAHSHLANSMIKNGGVLTEFPSNTTADKERFPMRN
                     RIIAAMSDALVVVETARKGGSMITADMAFNYNKDIFAFPGRTQDKYSQGCNYLIKSQK
                     AGLIENAEDLAAQMMWDKIDSGKTVQTSMFVELSELERKVLSELQTKDTGIDELSYII
                     QLTPAEMASVLLEMEFKGLIKSIPGKRYILA"
     gene            5991..7457
                     /locus_tag="PLE23_03990"
     CDS             5991..7457
                     /locus_tag="PLE23_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMX82457.1"
                     /translation="MNKSFLANLLLLLSINIVIKPVFIFGIDRNVQNLVGEHDYGLYF
                     TFLTFTMIMQVLGDAGLQNYNNQQISSGNVSFSNFFPSVMAAKLLVSILYVLLVMGGG
                     YLLGYQADYNLLMWVMLGQLLSGILLLLRTNVSGSGHYRTDSIFSAIDRLLMIAFCGY
                     YLWSQVRQSDFTILLFAKLQLISYSIAILIVLGWIIFKLPTTDVRIKHIATIPILKKS
                     FPYAIVVLLMIIYSRSDIILLKNMLEDGSKEAGIYAAGYRLLDAMNMVGFLAGSLLLP
                     MFARLMNDKKQTEELFILSLKILLLFTVIVAVTVISFRTQIMQLLYVNATEYWGNVLG
                     LLMVSFVSMTLSYVSGTYLTASGKVRHLIGSFLVAIIINLVLNLILIPRNKVEGVAVA
                     SAVTQLFILIVQMNKSMKYAGIRISKDYLVKFGVFLFMFLAISLLAGKILTINWYYQL
                     IITPIFGLLLAWLFKILPEELLNVVNGRKFLNRHFHQN"
     gene            7461..8585
                     /locus_tag="PLE23_03995"
     CDS             7461..8585
                     /locus_tag="PLE23_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82458.1"
                     /translation="MNNNDNLIGIVATLFKWRKPIIYLVISATVISIGASLLLPNYYS
                     STTTFYAASTDLAKPEMIFGLAGKAMEYYGSTDDIDRLLTIAESNELKDFLVDSFRLM
                     EHYDIDSNDIKSRYKVMEALNELYSVQKTKYDAIEITVEDKDRILAAQMVNTARDKIN
                     EFATSLIKESQKRLMSTFEKSMDEKQKEIVGLDNNLIDSRKKYGILNIESQSGMLGVL
                     ISRKESALVGSKGKLQIYQQMPNANKDTIMNLKANIKGLEDELRELTSPTSTTSLNLS
                     KFNDGLSKIEMLQQLHLQSRNRLSYDLDRYNMIKNAYESVTPSLILIEKGQVPIVKSR
                     PKRALIVLASVLATLLVSVIGVLLINNYSDVDWNRELYGK"
     gene            8551..10053
                     /locus_tag="PLE23_04000"
     CDS             8551..10053
                     /locus_tag="PLE23_04000"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMX82459.1"
                     /translation="MLTGTANCMANDVLPQAVPGLTRQNKILFTTLGVVTILSVLLAV
                     GLDKLWILVIPFIVLTVYASIIDLRLVFFLLFATIPFSTEVSFKNGLATDFPVEFFVI
                     YLMFAYLAYLLSNVKNISSRFFRHPLTLLLIFHTLWIGVTCLHSYNIVVSFKFFLAKI
                     WYVVTFYFLAGMVIKHLKDLRILFWCVFIPTFITVCIVLFRHAEFNFDFKHVNHLFYP
                     FYRNHVDYACLLALLFPYIFYHTLWYQKWSNKWLFLVFSLVFIFVAIYLSYTRAAILA
                     LVIAAFALYAIKYRFIKPAMILAFGLLIGLILYLGKNNKYLGLAPDYNKTITHQNYED
                     LLEATYKLEDISTMERVYRWVAGFQMFNREPWFGFGPGNFYFNYKSYTVSSFQTYVSR
                     NPEKSGIHSYYLMTLVEQGIVGLSVFMLLIFYALAKAEVVYHSLKDRESRFFLLSGVM
                     SFVVILSLLLINDMVETDKVGPFFFLSLVIITNMDLKVQEEKSSAKSESE"
     gene            complement(10081..10716)
                     /locus_tag="PLE23_04005"
     CDS             complement(10081..10716)
                     /locus_tag="PLE23_04005"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HMX82460.1"
                     /translation="MFLEPHFKGQRSGWIEVICGSMFSGKTEELIRRLKRAKIANQKV
                     EIFKPAVDQRYDKTNVVSHDMNTILSTPVSHSSKITELAEGFSVVGIDEAQFFDQGLV
                     DVCQELALKGTRIIIAGLDMDYLGKPFGPMPDLLAVAEYITKVHAICSHCGNLATHSY
                     RLTAVADQVMLGAKQEYEPRCRTCYQMGNILHFNPIKLREGTDDVYPDLFS"
     gene            10960..12096
                     /locus_tag="PLE23_04010"
     CDS             10960..12096
                     /locus_tag="PLE23_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82461.1"
                     /translation="MKANQPINRNFPIINFEKYRLGAALLSIVIVSLMLSSCKKDVLD
                     NQVVVEDLKQGVHVKTLEFIEKAKTISSEKKIEMRDGGYYSSTEAFDLINDALNYNYC
                     RPADEYLRTRVFKDSFNITFNPVNGISEIDLLNLYNAAANKAGEHYYGMSDSTKAAFM
                     FDVRQAGNPSGNNLPVYVYFMMTAGNCDTPTSYPYDETDYWWWAWDAGKCDDTYQGDD
                     APDIFRCDLRKNKLYKNKTASYYFTDRYSVSLAAFDGYPGGGFEMSDINSDQTIELND
                     LYNPLDSITNDNYLDYLLFKSKGDLDLCLDPVEMNFYYISSANLIDLNLPFNKVIGNI
                     DYGYNLTLSLPGNYFHVMRADYYKVNLIMGTSNATPLPCMGCNP"
     gene            12130..>13501
                     /locus_tag="PLE23_04015"
     CDS             12130..>13501
                     /locus_tag="PLE23_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82462.1"
                     /translation="MNIKSNFVSILLLIFVTGVQAQDYGYYPFPEHPSQMITQVFTVG
                     KDRFAVLPKEFFDQFGDASICSLSADNQLKICTSLNTDIVYDDIINGDNGYIFTGIIY
                     NLNYPQPDTLVIELRSLNFDLISVSKFSLGKEFDNLCVVINDSIRIFAACADVVPKNF
                     YEFVIPKDLDISKITIKNPVLKSELIFSVIESEDGEGYVCCFGSGVYLVDKHFNILKK
                     LDTVKHSWVNSVVPALDSGYVCFGQCKTTISPKFTGLGITKIGKKMDYVGGDVFAYDV
                     TAAGPVQFPIAKDNEHYYVAGMLGVWDNGINPYLDKTPNYFYIGKYDKDINRIWLKKH
                     GGERHYFLNGIIADSLGGCCVYGFVREPGITDGKEGQAIPFIMCFNDQGELTSYTTPQ
                     LPEERLFSIKGNPAYNSIILGNIYGQSGYTYSIFDTNGHKVSAGVLNTDDAVLDVSKL
                     SAGMYLL"
BASE COUNT         4003 a         2491 c         2931 g         4076 t
ORIGIN      
        1 ttatgaaaac catcatagaa ccattcagga taaaatctgt agaacccatc cgtttcaaca
       61 cagccgaaca aagagctgtt ttgctccgca aggcgcatta caatcccttt tatcttcact
      121 cagatgacgt gatgattgac ctgctcaccg acagtggtac cagtgccatg agtagcgacc
      181 aatgggcagg tatcatgcac ggcgacgaat cgtatgccgg tagcccgagt tttttcagat
      241 ttgagaaaac catccgcgac atcactggta tgcctttcat tatacctaca catcagggtc
      301 gtgcggcaga gaaaatactt ttttcaatca tgggtggtga gggcaaatat gtattgtcca
      361 atacactttt cgatactacg cgtggcaata tcgagttttc gggtgctgta ggcattgacc
      421 tggtatgcga agaaggcaaa cacccaactg tggcagcacc atttaagggc aatatggata
      481 ctgcggctct tattaaggaa atagagcggg taggagccgg caatgtgtcg gtagtagtca
      541 tgaccgtcac taacaattcg ggtggtggcc agccggtgag tatgcacaac atccgcgagg
      601 tgagcagtat ttgtaaggca aatggcattc cgtttttcat tgacgcctgc cgtttcgcag
      661 aaaatagtta tttcatcaaa aaacgtgaag cgggctatga atcgaagtcg gtagaagaga
      721 tagcacagga gatgttttct tatgctgatg ggtgtaccat aagcgccaag aaagacgctt
      781 ttgccaacat cggcggattc ctggctgtgc acaacgaaga actcgcagcc aaatgccgta
      841 acctcctcat cattaccgaa ggttttccta cctacggtgg tttggcaggt agagacctcg
      901 aagccatagc catagggctg cgtgaagtac tcgaagaaga ctacctgcag tacagaataa
      961 ggagtatgga gtatttgaca gaaaaactgg tggctgccgg agtgccggta atgcagcctg
     1021 caggtgggca cgccgtttat ctggatgcca aacagtttat ggatcacata ccggtgaacc
     1081 agtacccggg tcaggtgctt gcgtgcgagc tctatgtgca aggaggcatc cgcgcgtgtg
     1141 agatcggctc acttatgttt ggcaaatatg acatagatgg caaactcata ccggcacaac
     1201 tcgagcttgt aagacttgcc attccccgaa gggtatatac acaaagccat atcgattatg
     1261 tagcagaagt tatcattgag gtttttgcca atcgtcatgc tcagaaaggt atgcggataa
     1321 tagaagaacc accaatgatg agacatttta cagtaaaaat ggaaaaaatc ggctaatacg
     1381 gtatgtattg gcaggtttca gttgattttt gataaaattt gaataacttt gcagccaata
     1441 aataattacc acaatgggaa aaggagacaa gaaatcaaaa aaaggcaaga ttatcctggg
     1501 ttcgaacggt aactcaagac caaaacctaa caaagtcaga aaagctaaag ctcaggctta
     1561 ataccatatc ttgcaacacg aaaagctgaa cattctgtgc aaggtgttca gcttttttgt
     1621 tggtaaaatg cattcatttc atgaaatgga atcctgcacg atatgaagct caacatcagt
     1681 gccaataaga taagcgtcag gcacaatgag acagacattc aaccatttgc cgaaatgagg
     1741 caagtttttt atcctccaca ttttggtaac gcttatgtag ttaaaaatga gcaaacacgc
     1801 aaaaagcaac gaattagcat ccgggaatcc attttttatt gatatcattg aatcataatt
     1861 caccaaagcc ggtatagccg tatggaaagt gtcctgaaat catttctaca atttctgcaa
     1921 aatgcaacca tcggccagtg gatgatagtg gtggcaggcc taccactcat cggttacatg
     1981 atatttatga tttttgcatt cggctggaaa agcagaccgg tgaaaggccc tgacaggtgg
     2041 tatgagtatg taatgctgtc ttatctgttg agttttttat ggatgtggat gattagtgca
     2101 agggtgcagg acagggcaaa ttttctaagc tttctgcctg tcaatctgat attgtggccc
     2161 gggcccgtgt tgtttcattt tataaaaagt aaattgtata gaaactttcg gttccgccca
     2221 agagatttga aacatttcat atttccgctt gcacatttga gtttttattt ttttgcattt
     2281 gctttgcctc ctatgcggaa atacaattta tttctgcacg aataccatgc tatttacaag
     2341 ccatttgaag atttttcgtt tggcgtcatt atgtttttat attgttattt tgcttatcgc
     2401 ttcataaagc atgagcaatg gtctataaac gagtcaacgc caaaagcaga gatagtgaag
     2461 ataagctggt tgcagcggtt tttcaaggta ttcttcttaa gttctttcgt tcatcttgcc
     2521 tacgggttat tttctatggc gaatgccttt atttttagaa ttgtatttga gaataattat
     2581 ctcgaacttc ttcatatttt tgtgtttatg atatgtatgg gttggctcgg atttcatgct
     2641 gtgttaatga aagcattacc caaatccaat atttaaataa ttaaacttgc cgataccaac
     2701 atcttgcatg ataattcctg aacctatttg gaaaaatgac gtaaaacttg aacttagtaa
     2761 accttgttaa cagatttata tggcaaagca agtagcaaaa cctaaaccga tagtaaaacc
     2821 agcaccggtt tataaaaaat tcacatacga atcaggcttt tttaaaaaat actggcaaca
     2881 gatagctgta ttgacggctc tggcggtagg attgtacatt tattcggtca ctttcgatta
     2941 tgtactggac gaccagatcg tgatgaccaa gaacgaatat gtcaagaaag gcgtagctgg
     3001 tataggagat atcctcaaaa cagaaagtat gcagggatat tttggcgaac agaaagacct
     3061 ggtagtaggt gcccgatata gaccattgtc tattgtcacc tttgcaatag aataccagat
     3121 gtgggggctt aaccctgtga tgagccactt gatgaacatc ttgttgtatg cccttacggg
     3181 aatatttctg tacagaattc tattgatgtt gttcccgaaa aaggatgagc agaattcatt
     3241 tttcacgata ccgtttgttg cttctttgct gtttgtgacc caccctatac atagtgaagt
     3301 agtagccaat gtaaaaggac gagacgaaat catgaccttg ctaggggcac tgggaagtgt
     3361 gtattatacg ctcaaatacc tgtccaaccg aaaaagcctg tatttggtac ttagcggtat
     3421 catgtttttc cttgggctga tgagtaagga aaacgccatt accttccttg cagtgatacc
     3481 ggcaacggtt tattttttta ccaataccga aggttcggtc aacttcaaga cagtggtgcc
     3541 attgctgatt gctactgtag tgtatttgct gatacgttat tctgttattg gctatttcct
     3601 gagcagtggg cagaaagtta ctgacctgat gaacaatccg tttgcacaga tgacagggtc
     3661 agaaaaaatg gcaaccatat tttatacatt gctgatgtat cttaaactgt tgattttccc
     3721 acatccgctt acgcacgact attatcctta tgccatacct aaacttaatt ggtcaagtat
     3781 atgggcgata ttgtcgctac taatacacat tggtttagct gtttttgcca ttaaaaactt
     3841 taaaaagaaa agcctgcttt catattgtgt aatattctac ctggcaacat tgtcgattgt
     3901 gtccaatatt gtttttccgg taggaacatt catgaatgag cgtttcgtgt atatttcatc
     3961 tatagcaatg tgtctggctg gtgcctggtt gtttgttaat tacattcctg ctatacagaa
     4021 tggtaggttg aaaagccttg gattggcact cacagcactt tttgctttag ctttttgtgc
     4081 caaaactata gcacgtgtgc cggcatggaa gaataagctg tcgctcaacg tagcagccat
     4141 caagtattca ggcaacagtg cacgggccaa ttgcttcatg ggtacggctt tgttcgaaga
     4201 gtatcaagta gagaaagatg aggctaagag aaaagcgatg ttagctgata ttaacaaata
     4261 catcgatcgg tcactggaga tttaccctga ttattattct gccctacaga tgtattcggg
     4321 tgtggtggga gccaattatg cacaggatgg taatctcgac aaggctcttg atgcttttta
     4381 taaaatactt agcagacgta ccaccatcac atacgtagat cagtttcttg attatctggg
     4441 caattcgcct acagtcgatc agaaaaaaat gcaagatttt tattttaagc ttggttatga
     4501 tttgttctac aaacagaaga aatactataa caatgctgca aaatatctgg ataaggcatt
     4561 gaagtacaac gacctcgatc ctaaagccaa cttcgctatg tcattagtaa tgtctgatat
     4621 cggagattat gaaaaagccg accagtttaa acgggtggca ttaacttcag atccgggcat
     4681 tattaccttc tttgaacaag atattatgag gaaataagcc aaaagaattg ataataatac
     4741 attcgggcaa gcatcaataa acagattgct tgcccctttt gttttgatat atacgatagc
     4801 ccgggcggat cattttgata agtttttttc atagaaagca ctcagcttgc aggatgttgg
     4861 ttatttttgg cagatgaatc aagaagagct tttttacagg attgcaataa ccatgcttcc
     4921 gaaggtgggt ccggtgacag ccagaaactt gataagctat tgtggtggca ccagagaagt
     4981 attcgaaaca tccacaaagg cactgatgaa aatacccggt gtaggtgcag ccatgagcaa
     5041 aagtattctt cagcacgatt cgtttgcccc ggctgagcaa gaactggcat ttatcgacaa
     5101 acaaaatatt aagcccatat tttatctgga tgatgattat ccaaaaaggc tacaaaacgt
     5161 ttatgacgca cccgtactct tatattttag aggcgatgca acactcaatg cagccagaac
     5221 agtaggcatc gttggtacaa gaacgcccac accgcacgga atcgccaatt gcgaagcgct
     5281 tgttgaaggg ctcgctaagt ataatgtaca aataataagc ggacttgcac atggtataga
     5341 tggtacctcg catagggcag cagtggcagc cggtatctcc aatatcggag tggtagctca
     5401 tggtcttgac catatttatc cggccgcaca cagccatctg gcaaacagca tgataaaaaa
     5461 cggtggcgta ctgactgaat ttccaagcaa tactacagcc gacaaggaac ggttcccgat
     5521 gcgcaaccgg ataatagccg ctatgtctga cgctctggta gtggtagaga cagcccgcaa
     5581 aggtgggtca atgataacgg cagatatggc atttaactac aacaaagata tttttgcatt
     5641 cccgggtcgt acccaggata aatattcgca aggctgcaat tatcttatta agtcgcaaaa
     5701 ggccgggctg atagaaaacg cggaagatct ggctgctcaa atgatgtggg acaagattga
     5761 ttcgggcaaa accgtacaga ccagcatgtt tgtggaactt tcagagctcg aaagaaaagt
     5821 actgagcgaa ctacagacaa aagataccgg cattgatgaa ctgtcttaca taatacagct
     5881 tacaccggcc gagatggcat cagtattact cgaaatggaa ttcaaaggcc ttattaaatc
     5941 tataccgggc aagcgttata ttcttgcata aagtaactga ccattatcgg atgaataaat
     6001 cttttctcgc aaatctgttg ctgctactgt ccatcaacat cgttataaag cccgtgttta
     6061 tatttggtat tgacaggaat gtacagaatc tggtaggcga acacgactac ggattgtact
     6121 ttacttttct tacatttacc atgattatgc aggtgctggg tgatgccggg ttgcaaaact
     6181 ataacaatca gcaaatatcc tccggcaatg tatcgtttag caattttttt ccttcagtga
     6241 tggcagccaa gttgttggtg agcatacttt atgtattact tgtaatggga ggtggatatt
     6301 tgcttggcta ccaggccgat tataatctgt tgatgtgggt gatgttgggc cagctgctga
     6361 gtggtatatt gttgttgtta cgcaccaatg tgtcgggtag cgggcattat cgtaccgata
     6421 gtatattttc tgccatagac cgtctgctga tgatagcatt ttgtggttac tatctatggt
     6481 cacaagtgcg tcagtccgat tttactattt tactgtttgc taagctacag cttatttcat
     6541 attctatagc aatcttaatc gttcttgggt ggatcatttt caaactacca acaacagatg
     6601 tacggataaa gcatattgcg acaataccga tattgaagaa aagttttccg tatgctattg
     6661 ttgttttgct gatgatcata tatagtcggt cagatattat tttattgaaa aatatgcttg
     6721 aggacggcag taaagaagca ggaatatatg cagccggtta tcgcttgctc gatgcgatga
     6781 acatggtagg ttttttggca ggaagtctgt tgctcccaat gtttgccagg ctcatgaatg
     6841 acaaaaagca aacagaagaa ctttttatac ttagccttaa gatactgttg ttgttcacag
     6901 taattgtagc agtaacggtt atcagctttc ggacacagat tatgcaattg ctgtacgtca
     6961 atgctacaga atactggggc aatgtgcttg ggctgctgat ggtgtcgttt gttagtatga
     7021 cattgtcgta tgtgtccgga acctatctga cggccagtgg caaagtcaga catctgattg
     7081 gtagtttttt agtagcaatt atcattaatc tggtattgaa cctgatattg ataccaagaa
     7141 ataaggttga aggggtagct gtggcaagtg ctgttacaca gttattcata ttgattgtac
     7201 agatgaacaa aagcatgaag tatgccggta tccgtatctc aaaggattat ctggtaaaat
     7261 ttggtgtttt tttgttcatg tttttagcca taagccttct ggcgggaaag attttaacta
     7321 taaattggta ctaccaactg ataattactc ctatttttgg gcttttattg gcgtggttgt
     7381 tcaaaatact acctgaagag cttctgaatg tagtgaacgg gaggaaattt ttgaacagac
     7441 attttcatca aaactgatac atgaataata acgacaacct gataggtata gtagctaccc
     7501 tattcaaatg gcggaaacca attatttatt tagtaatatc ggccacggtc ataagcatag
     7561 gtgcttcatt gctgttaccc aattattaca gctctactac tacattttat gctgcaagca
     7621 ccgatcttgc caagcccgaa atgatatttg gccttgcagg caaagctatg gaatattatg
     7681 gtagtacaga tgatattgac cgcctgctta ccattgcaga gtcaaatgaa ttaaaagact
     7741 ttcttgttga tagttttcgc ctgatggagc attatgacat agacagcaat gatattaagt
     7801 cccgctacaa ggttatggaa gcactgaatg aactttactc tgtacaaaaa accaaatacg
     7861 atgccattga gataacggtg gaagacaaag acagaatcct tgctgcacag atggtcaaca
     7921 cagctcgtga caaaattaac gaatttgcca ccagcctgat taaggaaagt caaaaacgtc
     7981 tgatgagtac cttcgaaaaa tcaatggatg aaaaacagaa ggaaatagta ggtcttgaca
     8041 ataacctgat agacagcagg aaaaaatacg ggatcctcaa catcgaatca cagagtggta
     8101 tgctcggagt gctcatttca agaaaagaat cagcccttgt aggtagcaag ggaaaacttc
     8161 agatatacca acagatgcct aatgccaaca aggataccat tatgaacctc aaggccaata
     8221 tcaaaggtct tgaagatgaa ttgcgcgaac tcacgtcgcc tacaagtact acaagcctca
     8281 atctctcaaa atttaacgac ggattgtcaa aaatagaaat gctgcagcag ctgcatctgc
     8341 aaagtcgcaa tagacttagt tatgaccttg accgttacaa tatgattaaa aatgcttacg
     8401 agtcggtaac accatcactg atattgatag aaaagggcca ggtaccgatt gttaagtcgc
     8461 ggccaaagag ggctctgata gtactggcct cggtgctggc tacattgctt gtttcggtaa
     8521 taggcgtact tctgattaat aactactctg atgttgactg gaaccgcgaa ttgtatggca
     8581 aatgatgtat taccacaggc ggtaccggga ctgaccagac agaacaagat actttttact
     8641 acattgggtg ttgtaaccat attgtccgta ttgctggcag tcggactcga caagctatgg
     8701 atactagtta taccattcat agtgcttacg gtgtatgctt ccattattga tctcagactt
     8761 gtattttttt tattgtttgc taccataccg ttttccacag aagtatcttt caagaatggg
     8821 cttgcaaccg attttccggt cgaatttttt gtaatatatc tgatgtttgc atacttggcc
     8881 tacctactca gtaatgttaa gaacataagt agtagatttt tcagacatcc gcttacgctt
     8941 ttgctcatct ttcacacatt gtggataggc gttacctgtc tgcattcgta caatatagtg
     9001 gtatcattta agtttttttt ggccaaaata tggtatgttg tcacatttta tttcctggca
     9061 ggtatggtca taaaacatct gaaagacctg cgtatattat tctggtgcgt atttattcct
     9121 acgtttatta cagtttgcat tgtacttttc agacacgcag agtttaattt tgacttcaaa
     9181 catgtgaacc atctttttta cccgttttac cgcaatcatg ttgactatgc ctgtttgctt
     9241 gctttgttgt ttccatatat attttaccat acactttggt atcagaaatg gagcaacaag
     9301 tggcttttcc ttgtattcag ccttgtattc atttttgttg ctatttatct ttcttatacc
     9361 agagctgcta tacttgcctt ggttatcgcg gcttttgctt tgtatgccat aaagtacagg
     9421 ttcataaagc ccgcaatgat tctggcattt ggcctgttga taggcctgat attatatctg
     9481 ggtaaaaaca acaagtatct tggtctggca cccgattaca ataagaccat cacgcatcag
     9541 aattatgaag atctacttga ggctacatac aagctcgagg acatctctac gatggagcgt
     9601 gtgtacaggt gggtggctgg gtttcagatg ttcaacagag agccatggtt tggttttggt
     9661 cctggcaatt tttattttaa ctacaaatca tacacagttt cgagttttca gacctacgta
     9721 agccgcaatc ccgaaaaatc gggcatacac agctactatc tgatgacgct tgtagaacaa
     9781 ggcatagtcg gcctttctgt ttttatgctg cttatattct atgcgcttgc caaagcagag
     9841 gtagtctatc actcactcaa agaccgagaa tctaggtttt ttcttttgtc aggcgttatg
     9901 agctttgtag tgattctttc tttgttgctt atcaatgata tggtagagac tgacaaggta
     9961 gggccgtttt ttttcctgtc tttagtcatc atcactaaca tggacctcaa ggtacaagag
    10021 gagaaatcaa gtgcaaaatc agaaagtgag taatgccgga gttcgacgga agtgtaatga
    10081 ttatgaaaac aaatcgggat aaacgtcgtc cgtaccttct cttagtttta tggggttaaa
    10141 atgcaggata ttgcccatct gatagcaggt acggcagcgt ggttcatatt cttgtttggc
    10201 accaagcatg acctggtctg ctacggcggt aaggcggtat gagtgggtgg ccagattgcc
    10261 acagtgcgaa cagatggcat gtaccttggt gatatattcg gctaccgcaa gtaagtcagg
    10321 cattgggcca aatggtttgc ccagataatc catgtcgagc ccggcaatga tgatgcgcgt
    10381 tcctttcaag gcgagttcct ggcagacatc taccaggcct tggtcaaaga actgtgcttc
    10441 gtcgatacca actactgaaa acccttctgc cagctctgtt atttttgaag aatggctcac
    10501 aggcgtggat aatatggtgt tcatatcatg actcacaaca tttgttttat cgtagcgctg
    10561 gtcaacggca ggtttaaata tttctacctt ttggttggca atttttgccc gtttcaacct
    10621 cctaatcagc tcttctgttt tgcctgaaaa catcgacccg cagatgactt caatccaacc
    10681 actgcgctgt cccttaaaat gaggttctaa aaacataata acagataaat tttccggtac
    10741 attaacaaat gaagataaga aaaagttaaa aaactttgtg atcacagtct cctgtcacgc
    10801 acaaaactaa gaaaaaagct tattagttct gattatttat actctgggta agagatgact
    10861 acatttacgc attaaaagtc ctgaaaaaat tttgttaaag ttttggatat tacaaaaaat
    10921 agtattaatt ttggatttta ttaaataaaa ccatttatta tgaaggccaa ccaaccaatc
    10981 aaccgaaatt ttccaattat taattttgaa aaatatagac ttggagcagc attgctcagt
    11041 atcgtaattg tttctttgat gttatcttcc tgtaaaaaag atgtacttga taatcaggta
    11101 gtagttgaag atttaaaaca gggtgtacat gtaaagacgc ttgaattcat tgaaaaggca
    11161 aaaacaatta gctcagaaaa gaagatagaa atgagagacg gcggttatta ttcaagtaca
    11221 gaagcattcg atctgataaa cgatgctttg aattataatt attgccggcc ggcagatgag
    11281 tatcttcgta cccgagtatt caaagattcg tttaatatta cttttaatcc tgtgaacggg
    11341 atatcggaga tagatttact gaatctgtat aatgcagcag ccaacaaagc aggggagcat
    11401 tattatggta tgagtgattc taccaaggca gcctttatgt ttgatgttag acaagcaggt
    11461 aatccatcgg gtaataacct gccggtatat gtatatttta tgatgactgc gggcaactgt
    11521 gatacgccaa cgagctatcc ttatgatgag acggattatt ggtggtgggc atgggatgct
    11581 ggtaaatgtg acgatacata tcaaggcgat gatgcacctg atatttttag atgtgatctt
    11641 agaaagaata aactgtataa aaacaaaaca gcttcgtatt attttacaga tagatactct
    11701 gttagtcttg cagcgtttga tggttatcct ggaggtggat ttgagatgtc tgacataaat
    11761 tctgatcaaa ctattgaatt gaatgatttg tacaatccac ttgatagtat aacaaacgat
    11821 aattatcttg attatttgtt atttaagtcc aagggtgatt tagatttatg tttagatccg
    11881 gttgagatga atttttatta tattagctca gccaacttga ttgatttgaa tttacctttt
    11941 aataaggtaa taggtaatat agattatggt tataatctaa cgctttcatt accaggaaat
    12001 tattttcatg ttatgcgggc agattattat aaggtgaacc tgataatggg tacttccaat
    12061 gcgaccccac ttccttgtat gggttgtaat ccgtgaggag tctattttta aaaccacaaa
    12121 tttaagaata tgaatattaa gagcaatttt gtttctatac tcttgctgat ttttgtcaca
    12181 ggagttcagg cacaggatta tggatattac ccctttccag aacatcccag ccaaatgata
    12241 actcaggtgt ttacggttgg taaagataga tttgctgtct tgccaaagga attttttgat
    12301 caatttggag atgcttcaat atgttcctta agcgctgata atcaactaaa aatttgtaca
    12361 agccttaata cagatattgt gtatgatgac attattaacg gagataatgg atatatattt
    12421 actgggatta tttacaatct gaattaccca caaccagata cgctggtaat agagctccgc
    12481 tcattgaatt tcgatttgat atctgtgagc aaattttctc ttggaaagga atttgacaat
    12541 ttatgtgtag tcattaatga ttctatacga atatttgcgg catgtgctga tgttgtacca
    12601 aaaaactttt acgagtttgt aataccaaag gatttggata ttagtaagat aacaatcaag
    12661 aatcctgtgt tgaaaagtga gcttatattc tcggtaattg aatctgaaga tggtgaaggg
    12721 tatgtatgtt gttttggtag cggagtatat cttgtagata aacattttaa tatacttaaa
    12781 aaacttgata ctgtaaagca cagttgggta aatagtgtag taccagccct ggatagtgga
    12841 tatgtgtgtt ttggacagtg taaaaccaca atttcaccta agtttacagg tcttggaatt
    12901 actaaaatag gcaaaaaaat ggattatgta ggcggagatg tttttgctta tgatgtgacc
    12961 gctgcgggcc ctgttcagtt tcccattgca aaagataatg aacattatta tgtagcagga
    13021 atgctcggag tgtgggataa tgggattaac ccttatttgg acaaaacgcc aaattatttt
    13081 tatatcggaa agtatgacaa agatattaac agaatttggc taaaaaagca tggtggtgag
    13141 aggcattatt ttctgaatgg tataattgca gatagtctgg gtggatgctg cgtttatggg
    13201 tttgtaagag agcccggcat cacggacggt aaggaaggcc aggccatccc ctttattatg
    13261 tgctttaacg accaaggaga gctgacatct tatactaccc cacaactacc ggaggagcga
    13321 ttgttcagta tcaaagggaa ccctgcctat aacagcatca tacttggcaa tatctacggg
    13381 caatcgggct atacctatag catattcgat acaaacggtc ataaggtatc agccggtgta
    13441 ttgaatacag atgatgcggt gcttgatgta agcaaactgt ctgcgggtat gtatctgttg
    13501 t
//
LOCUS       DAOQQY010000046        13230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1593_length_13230_cov_5.172903, whole genome shotgun sequence.
ACCESSION   DAOQQY010000046 DAOQQY010000000
VERSION     DAOQQY010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13230
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1593_length_13230_cov_5.172903"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="PLE23_04020"
     CDS             <1..904
                     /locus_tag="PLE23_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82463.1"
                     /translation="ILDVTGYIINDAKKDKFVVGDSAKVFTNAMRGRRISITNYNNKV
                     EAEGPLSFGSGLKYVNLQAAGKIESEFAVIDTTLEADKVKLPPVNIQTMLGIELTIPE
                     VLLTAMVNDLRTSTFESNFIDYNRGFEFYDHAMSEFITDDAVLTPLLSTMKARTLELP
                     KEFKKFSLFFSKLDLIWDADYSSFVSSKDVNGLAAINGEMLNKMVTSSVEIKMPSNED
                     DRLYIYIKGPNENFYFFGYSQGILSVTSNNTVFTDAFLKLKKKELVYKKENDETYEIQ
                     AVSPETADLFVQRAKAAGKPKTEN"
     gene            complement(1345..1842)
                     /gene="purE"
                     /locus_tag="PLE23_04025"
     CDS             complement(1345..1842)
                     /gene="purE"
                     /locus_tag="PLE23_04025"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HMX82464.1"
                     /translation="MNNKVGIIMGSDSDLTVMKEAASILDHFGVTYEISIVSAHRTPD
                     RMFEYAKTAHTRGLKVIIAGAGGAAHLPGMVAAITILPVIGVPVKSSNSIDGWDSILS
                     ILQMPNGVPVATVALNAAKNAGILATQILATDNNSLVKKLETYKAEMHEQVIEKNRNL
                     DKKKE"
     gene            complement(1842..2993)
                     /locus_tag="PLE23_04030"
     CDS             complement(1842..2993)
                     /locus_tag="PLE23_04030"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HMX82465.1"
                     /translation="MSKPVSEIKIGILGGGQLGRMLVQAGKEWNLDFYILDESASFPS
                     GNVGAGFVTGNFKDYDDVYKFGKDKDIITVEIEHVNTDALLQLQREGVEVHPNPASLN
                     IIKDKGTQKLFYKNHDLPTADFQIFESKKDIFLALESSTLAYPFVQKSRLAGYDGKGV
                     AIIRNDNDVLEIMDVPSVTEKLVDISTEISVIASRNKNGQIRCYPAVDMEFHNKANLV
                     EFVYFPSKISKEKQKEAENLAATIINELDICGLLAVEMFLDKDGKILINEIAPRPHNS
                     GHITIEASITSQFQQHLRGICNLPLGDTRFHSPAVMVNLLGAKGHSGKTKYSGMDECL
                     QIDGASIHLYGKATTKPYRKMGHATVLDKTRAKATRNAKQIKKFLKIHT"
     gene            3071..4405
                     /locus_tag="PLE23_04035"
     CDS             3071..4405
                     /locus_tag="PLE23_04035"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF006688.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HMX82466.1"
                     /translation="MAGIIIKNTKIVNENIIIEGDLLIRDGRIEKVGGIISNTNAQEI
                     DGTGLHTLPGIIDDQVHFREPGLTHKATIESESKAAIAGGVTSFMEMPNTIPAAVTND
                     LLEDKYNIANQSSWANYSFFLGVSNSNLDEVKKVNVKNVCGVKIFMGSSTGNLLVDKV
                     EALEGVFRESPTLIATHCEDEATIKSNFELAVEKFGNDIPPAWHPVIRNAEGCYLSSS
                     FAVSLAKKFDARLHILHISSGIETELFDNTKILSEKRITSEACVHHLYFNDTFYKTKG
                     NLIKCNPAIKSENDRLAVWDALLSDKIDIIATDHAPHTWNEKQLPYAKAPSGLPLVQH
                     SLPIMLNFYNQGKISLETLVRKMSHAPAVCFKIKDRGFIREGYHADLVLVNTQREQMI
                     NNSQTHYKCGWTPLAGDILKGTVEHTIINGLHVYEKGIFRRANGARLQFDRI"
     gene            complement(4489..5532)
                     /locus_tag="PLE23_04040"
     CDS             complement(4489..5532)
                     /locus_tag="PLE23_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HMX82467.1"
                     /translation="MNINDFDPNGVGLRNGNFIGLPFDEENSKIILFPVPWDVTVSYN
                     DGTSTGPANILQESVQLDLHDIDIDNAWQSGIYFAEMDDEILKKNNSLREKASSYIYF
                     LEEGGKLSGDSQTRKILDEINTESAKLNDWVKAKTAHWLDQGKLVGLVGGDHSTPLGY
                     YQALAERHAEFGVLHIDAHMDLREAYEGFEYSHASIFYNALKIPNITKLVQAGIRDYC
                     QAETQLVNSQNQRIEVYYDHYIKQAIYRGDKFSKIVKEMISNLPDKVYISFDIDGLDP
                     KLCPNTGTPVPGGFEFSEVCFIFEQIVESGRTIIGFDLVETGAAGNAWDGNVAARVVY
                     KLASWMAKSNGII"
     gene            complement(5599..7719)
                     /locus_tag="PLE23_04045"
     CDS             complement(5599..7719)
                     /locus_tag="PLE23_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMX82468.1"
                     /translation="MNDKLLNKLHLFLIVAGTLLLSCKTGGQDAHNNKTGNHLINESS
                     PYLLQHAHNPVDWYPWNDEALQKAQKEDKLIIISIGYSSCHWCHVMEKECFSDTAVAR
                     VMNDHFVSIKVDREERPDVDDIYMTACQLTNESCGWPLNAIALPDGKPIWVGTYFPKD
                     QWIRILEKFQSLKTENPGELQKNAAQITDYIKEMHKGEHVIGNIIPKSDIQNILQTIT
                     NKTDKVYGGRMGDSQKFPRTESYDFLLQSAYYYNNNPAKDAALITLDNIIYGGIYDHV
                     EGGFARYTVENKWRIPHFEKMLYDQAGIVSLLANAYKITGNETYKSRITETLQFTDTK
                     LSDKEGGFYSSFDADSEGEEGKYYVWTEAEIDALIKDPALNKTAKVYYNISTKGNWEK
                     GQNVLYADRNVNKTAAVLKTNISQLEEQLKKVNTILRTARDKRTAPHRDDKILTSWNA
                     AMCKAYLDAYTATGDRTYLDRARKNLDFLTTKVMQKDGKLFRNYKGGKATINGFLDDY
                     AFTIQALIEMYQATFEEKWLQQAKLITDYTIKHFYDATRLTFYYTDDSDSKLLSRKIE
                     TSDNDLPASTAVMATSLYSLGDLLYNDDYSTKAKNALARASTEINKTSSPESYASWLK
                     LASLIHRTPYEIAITGDNSEQFRAEMQKHYLPDAIYLGGKTEGTLELLKGKVQSGSTL
                     IYVCKEKVCKLPQKTVKEALAEMK"
     gene            8106..9383
                     /locus_tag="PLE23_04050"
     CDS             8106..9383
                     /locus_tag="PLE23_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMX82469.1"
                     /translation="MLSAQSIQIKYTEYDLPNGLHVILHEDHSTPIVAVSIMYHVGSK
                     NENPNRTGFAHFFEHLLFEGSENIPRGEFAKIVERSGGTLNANTSNDRTYYFEIMPSN
                     QLETSLWLESERLMHAKVDKVGIETQRSVVKEEKRQRVDNQPYGTLIEEIMKRMYTNH
                     PYRWMTIGSMEHLDAASEEDYVNFYKTFYVPNNAVLSIAGDINVEQTKKLIEKYFGPI
                     PKGTKPIFRPTSGDVPLTSEIKDTVYDNIQLPAVIEAYRMPSIKDEDFYAVRMLGQYL
                     SEGESSKMHKAIVDEQQKAVFVGAFPYPLEHEGVFITFGIANFGGDPGDLQKSMDEVI
                     FSTQNDLISENDFTKLRNSIETEFVSRNATMAGIAETLASAYTYFKNTSLVNTELDKY
                     MKVTREDIKRVANQYFNKNKRVVLYYMPKLKKS"
     gene            9398..11575
                     /locus_tag="PLE23_04055"
     CDS             9398..11575
                     /locus_tag="PLE23_04055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMX82470.1"
                     /translation="MKNLRILYILFLVLGLGTTQLGVAQVKSKTTTKTQTKAKPKATT
                     TKPKPMTDFRSQQPKAGPAPLIQISDYTVHSLKNGLKVIVVENHKLPRASFQLFIDND
                     PILEKDSKGYVDLAGQMLGKGTSKKSKAQLDEAIDFLGASFSANANGFFASSLTKHSE
                     KLLELASEAVLDPSFPEAEFQKIKSQSQSGLQQAKDDANTISANVASVLNFGKDFPYG
                     ENMTEKTLEKITLQQCKEYYNTYFKPNNAYFIAVGDIKAEKAIALAEKFFGGWTPGIV
                     PKNNYSAPKQNNQPMVSFVNKDGAVQSVINVTYPVVLKPGTEDVVRVAVMNNILGGHG
                     FTARLFQNLREKHAFTYGAYSTIGPDKLAGTFSASASVRNEVTDSAVTQFLNEMNRIR
                     TEKVTDLELSSIKASLAGDFSRSLERPQTVANFALSTIRFNLPKDYYKTYLARLDAVN
                     ADAVLQTAQKYILPNNANILVVGNKDEVADKLKQFAPDKKVNFYTAYGDKVVDSAPVD
                     LGNLTAGNVIADYVKAVGGVDQINKVKDVHFIAQTNVQGMAIQTDKYVKMPDKYAEKM
                     SMNGSVVQEQIVNGKKGIQGQMGQNSAMDEESLNAIIDEIGLIPQVKYEAEKYTLKLI
                     GTENVEGNDAYKIEVVSPKGAKKLEYYDVKSKLKLREVMSQTAGEKTVTMVTDFSDYK
                     AVDGIMIPHKAVITGGMMPVPIEMITNSVEINKGINDSMFKVD"
     gene            11583..12074
                     /locus_tag="PLE23_04060"
     CDS             11583..12074
                     /locus_tag="PLE23_04060"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HMX82471.1"
                     /translation="MIQVYITGGTFDKEYNFITGELYFKDTHLPDMFRLGRCTQDIDV
                     KTLMMVDSLEMTDDDREIILHNCKKSESDHIIITHGTDTMVRTGEFLAKANIDKTIVM
                     TGAMIPYAFGTSSDGFFNLGSALAFVQTLPKGVYIVMNGRYFLWNNVRKNKKTGFFEE
                     LEA"
     gene            12093..12326
                     /locus_tag="PLE23_04065"
     CDS             12093..12326
                     /locus_tag="PLE23_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82472.1"
                     /translation="MSMDALGLLFELIIAFISLGLFFLITGKWTTYNTRLNELIQSIN
                     NSSMKTLFKWTSLIVGVLMLINFILHVLIEIKK"
     gene            12509..>13230
                     /locus_tag="PLE23_04070"
     CDS             12509..>13230
                     /locus_tag="PLE23_04070"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMX82473.1"
                     /translation="MRFVQLSILLLFSFVASAQSSETDIFDPNHISEIRLTIKQQNYA
                     DILDSLKVYGAGMIVGDVNINGTKIKDVGIRYRGNSSYGFGNKRNPWQIKLDYINKSQ
                     KYQGVSSIKLSSALRDPSMVREVLGFEIARKYMVAPRANYTKLYVNDKYIGLYVNVES
                     IDKNFIERNNASRSNTFFKCSPDPDTKSNPGCKNKIYSALVYEQDPNCFLPNYTLISE
                     SGWDELIELTKVLEQSPANINS"
BASE COUNT         4123 a         2577 c         2498 g         4032 t
ORIGIN      
        1 gatattggat gtcaccggtt atatcatcaa tgatgccaag aaagataaat ttgtagtagg
       61 tgatagtgcc aaagtattta caaacgccat gagaggaaga aggataagta ttaccaacta
      121 caataataag gtggaagcag aaggtccgct cagttttggc tcaggtctaa aatatgtaaa
      181 ccttcaggct gccggcaaaa tagaatcaga atttgcagtc atcgacacta cactcgaggc
      241 agataaggta aaactaccac ctgtaaacat ccagacaatg ttaggtattg agcttactat
      301 tccggaagtt ttacttacag ctatggttaa tgacttgcgc acaagcacat tcgaatccaa
      361 cttcattgac tataatcgcg gattcgaatt ctatgatcat gcaatgtccg aattcatcac
      421 cgatgatgct gtgctgacac cgttgctgag caccatgaaa gctcgtacgc ttgaattgcc
      481 aaaagaattt aaaaagttca gcctcttctt cagcaagctc gatctcatct gggatgccga
      541 ttattcttcg tttgtttcga gtaaagacgt aaacgggctg gcagccataa atggtgaaat
      601 gctcaataag atggtgacat cgagcgtcga gatcaagatg ccttccaacg aagatgaccg
      661 actgtatatt tacatcaaag gcccgaatga aaatttttac ttcttcgggt attcgcaggg
      721 catactttct gtcacctcca ataatactgt ttttacagat gcattcctaa agctgaagaa
      781 aaaagaactc gtgtataaaa aggaaaatga cgaaacctac gaaattcaag cagtgtcacc
      841 tgaaactgca gatttgttcg tacaacgtgc caaggcagcc ggaaaaccta aaactgaaaa
      901 ttaatctaat ataattataa ttttacaaaa acgggatgat gtttaaaatt gcttcatccc
      961 gtttttaatt gttgacagtt tgaaagccga cattccggtt tacattccaa ctcaaaagtg
     1021 attttgtaac tgtttacaat cttggtgttt agattatgaa aatctatata gatatttttt
     1081 tttacttttt cagaaaactt ttcgaaagaa tatgttttgc tgaaatttat cacaaaaaca
     1141 gtaaattctt actgtaaata cctacaattt gtaactaaat ccttcatttc acaaccaatt
     1201 atgcttgtca caatttggcg ttgcagcaaa ggtatttaaa ttttatttat atatttttta
     1261 aaatattcaa tttttggcaa atgttttgct attattatat tgattgttgt tgtttacctt
     1321 tggatggagt aacctcgtag agtgttactc tttttttttg tccaaatttc tattcttttc
     1381 tattacttgc tcatgcattt cagctttgta tgtctcgagt ttcttaacaa ggctgttgtt
     1441 atctgtggct agtatctgag tagcaagtat tccggcattt tttgctgcat tgagtgctac
     1501 tgttgctacg ggcacgccat taggcatttg aagtattgag aggatagaat cccacccatc
     1561 tatcgagtta gaagacttga ccggaacacc tataaccggc aatatcgtaa tggcagcaac
     1621 catacctgga agatgtgcag cacctcctgc acctgcaata attaccttta atcctcgagt
     1681 atgtgcagtc tttgcatatt caaacatacg atcgggtgta cggtgggcag atacaataga
     1741 gatttcatac gttacaccga aatgatctag tattgaagcc gcttccttca tgactgtgag
     1801 gtcagaatcc gaacccatta ttatgcctac tttgttattc attatgtatg aattttaaga
     1861 aatttcttaa tttgtttagc atttctggta gcttttgccc ttgttttgtc aagtaccgta
     1921 gcatgtccca tttttctgta aggtttagtt gtagcttttc catacaagtg aatgcttgcg
     1981 ccgtctattt gtaggcattc atccattcca gaatatttcg tttttccgga atgtccctta
     2041 gctcctaaaa gattgaccat tacagcagga gagtggaacc tggtatcgcc caagggaagg
     2101 ttgcagatac ctctcaaatg ttgttgaaac tgagacgtaa tacttgcttc gatggtgatg
     2161 tgtccgctgt tgtgtgggcg tggagctatc tcgtttatca agattttgcc gtctttgtca
     2221 agaaacatct caactgcaag aagaccacaa atgtccagtt cgttaattat tgtggcagct
     2281 aaattttccg cttctttttg cttttctttt gaaatttttg aagggaaata aacaaattct
     2341 accagattag ccttgttgtg aaattccata tctacagcag gatagcatct tatctggcca
     2401 tttttattac gcgatgcgat gacagatatt tcagtagata tatctacgag cttttccgtc
     2461 actgacggaa catccattat ttccaaaaca tcattgtcat ttctgatgat tgccacacct
     2521 ttgccatcat aacctgccag tctcgatttt tgtacaaaag ggtaggcaag tgtgctagat
     2581 tctaacgcaa gaaatatgtc ttttttactt tcgaaaatct gaaaatcagc tgtgggcaga
     2641 tcatgatttt tgtagaataa tttttgtgta cctttatcct tgatgatgtt tagagatgcc
     2701 ggatttgggt gtacttccac accttctctt tgaagttgaa gcagagcgtc agtattaaca
     2761 tgctcaattt cgactgtgat aatgtcttta tccttgccaa acttatatac atcatcataa
     2821 tctttaaaat tgccggtgac aaagcctgct cctacattgc ctgaaggaaa actggctgat
     2881 tcgtcaagaa tgtaaaagtc cagattccat tcttttccgg cttgtaccag catacgacca
     2941 agctgacctc cgcccagtat ccctattttt atttccgaaa ccggtttaga catttaaaat
     3001 ttacttattt tgtgcaaaaa tacatttata aaaagtttgg caggtataat tgtcaagtaa
     3061 atcatttcaa atggccggca taattatcaa aaatacaaaa atagtaaatg aaaacatcat
     3121 aatcgaaggt gatctgttga tacgggatgg aaggatagaa aaggtaggtg gcattatctc
     3181 taacaccaat gctcaagaaa tcgacggtac aggcttgcat accttacccg gcatcattga
     3241 tgatcaggta cactttcgcg aaccaggtct cacgcacaaa gctactatag aatcggagtc
     3301 aaaggcggcc attgccggag gtgtcaccag ttttatggaa atgcctaata ccattcctgc
     3361 tgcagttacg aatgacctgc ttgaagacaa gtataatatt gcaaatcaga gttcatgggc
     3421 taattattca ttcttcctcg gtgtgtcaaa tagcaacctt gacgaagtga aaaaagtaaa
     3481 tgtaaagaat gtttgtggtg ttaaaatctt tatgggctca tcaaccggta acttgctcgt
     3541 tgataaagtt gaagcgcttg aaggtgtatt tcgtgaaagc ccaaccctta tagccacaca
     3601 ttgcgaagat gaggccacaa taaaaagtaa cttcgaactc gcagttgaaa aattcggtaa
     3661 tgacattcct cctgcatggc acccggtgat tcgtaatgca gaagggtgct acctgtcaag
     3721 ttcatttgct gtttcacttg ccaaaaaatt tgatgcacgg ctacacattc tgcacatctc
     3781 ttcaggaata gaaacagaac tattcgataa taccaaaata ctgtctgaaa aaaggattac
     3841 atcagaagcc tgtgtccatc acctttattt caatgacaca ttttacaaaa ccaaaggaaa
     3901 cctcatcaag tgcaaccctg caatcaagtc agaaaacgat cgcctggccg tatgggatgc
     3961 tttgctatca gataaaattg acatcattgc caccgaccat gcaccacata cctggaatga
     4021 aaaacaactg ccttatgcta aggctccatc cggtcttcca cttgttcaac attcgctacc
     4081 cattatgctc aatttttata accaaggaaa aatttcactc gaaaccctgg taagaaaaat
     4141 gtcgcatgca cccgccgtat gttttaagat aaaggacaga ggcttcatcc gagaaggcta
     4201 tcatgctgac cttgtgcttg ttaatacaca acgtgaacaa atgatcaata attcacagac
     4261 acactacaaa tgcggatgga caccattggc tggtgatata ttaaaaggca ccgtagagca
     4321 cactatcatc aacggacttc atgtttatga aaaaggcatt ttccgtaggg ctaatggagc
     4381 cagactccag tttgaccgga tttaaaacta ttagattttt taatacacta aattaaacca
     4441 ttcagtaaat caatagttta tctaatgttt attaaataaa agctttaatc atattatacc
     4501 attgctctta gccatccatg aagcaagttt ataaacgacc cttgctgcca catttccgtc
     4561 ccaagcgttg cctgctgctc ctgtttctac caagtcgaat ccgataatag ttcggcccga
     4621 ctctacgatt tgttcaaaaa tgaaacacac ctcgctaaat tcgaatcctc ctggaactgg
     4681 agtgccggta ttggggcaaa gttttgggtc aagaccatca atatcaaaac tgatataaac
     4741 tttatccggc agatttgaaa tcatttcttt gacgattttc gaaaatttat caccacggta
     4801 gattgcctgc tttatatagt gatcataata aacttctatc ctttggtttt gactgttgac
     4861 taactgagtt tcggcctggc aataatcgcg aatgcctgcc tgcacaagtt tcgttatatt
     4921 aggaatctta agtgcattat agaatatgga tgcatgtgaa tattcaaaac cttcataggc
     4981 ttcacgcaaa tccatgtggg catcaatgtg taacacacca aactccgcat ggcgctctgc
     5041 cagagcctga tagtagccga gcggtgtact gtggtctcct ccaaccaaac ctacaagttt
     5101 gccttgatct aaccaatgtg ctgttttggc cttaacccaa tcattcagct ttgccgattc
     5161 tgtatttatt tcatccagaa ttttccttgt ctgcgaatca ccggataatt ttccaccttc
     5221 ttcaaggaag tatatatatg aagaagcttt ttcacgtagt gagttattct tttttagtat
     5281 ttcatcatcc atttctgcga aatagattcc ggattgccat gcattgtcta tgtcaatatc
     5341 atgcaggtca agctgcacgg attcctgcag aatattggca ggccccgtgg aggtaccatc
     5401 attgtaggag acagtaacat cccatggtac aggaaaaagg attatcttgg aattttcttc
     5461 gtcgaacgga agtccaataa agttgccgtt tcgcaggcca acaccatttg ggtcaaagtc
     5521 gttaatattc atactgcaaa agtacgccaa cttattcgca gaaaagcata atgccccaaa
     5581 caattcagca gtacattatt atttcatttc agccaaagcc tcttttacag ttttctgagg
     5641 cagtttgcat actttttctt tgcacacata tattaaagta gagccggact gaactttccc
     5701 tttcaacagt tcaagcgttc cttctgtttt tccgccaaga taaatagcat caggcaaata
     5761 atgtttctgc atttcagccc tgaactgctc actattatca cccgttatgg ctatttcgta
     5821 aggagtacga tgaatgagag atgcaagttt taaccacgaa gcatacgatt ccggacttga
     5881 tgttttgttt atctcggtcg aagctcgtgc aagtgcattc tttgctttag tggaataatc
     5941 atcattgtac aaaaggtcgc ctaacgaata aagtgatgtt gccattacgg cagtagaagc
     6001 aggaaggtca ttgtccgaag tttcaatttt tctggacaaa agctttgagt cgctatcatc
     6061 tgtatagtaa aatgtaagtc ttgtggcatc ataaaaatgt ttgatagtat aatcagttat
     6121 caattttgct tgttgaagcc atttctcttc aaaagtagcc tgatacattt ctattaatgc
     6181 ttgaatagtg aatgcataat catctaagaa tccattaatt gtagctttac cacctttgta
     6241 atttctgaat aatttaccgt ctttctgcat taccttggta gtcaagaaat cgaggttctt
     6301 tcttgctctg tccagataag ttctatctcc ggtagcagtg tatgcatcca aataggcttt
     6361 gcacatggca gcattccacg aagtgaggat tttatcgtct ctatgtggtg cagttcgttt
     6421 gtcacgggct gtcctcagta ttgtatttac tttttttaat tgttcctcca gttgggaaat
     6481 atttgttttc agaacagcag cagttttatt tacatttctg tctgcataga gtacattctg
     6541 ccccttttcc cagttacctt ttgtagaaat gttgtaatac acctttgccg tcttattcaa
     6601 agcagggtct ttgataagag cgtcaatttc tgcttcagtc caaacgtaat atttaccttc
     6661 ttctccctcg ctgtctgcat caaaagaaga atagaatccg ccttctttgt cacttaattt
     6721 tgtatctgta aattgtagag tttcggttat tcttgatttg tatgtttcat tgccggttat
     6781 tttgtaggca ttggcaagca atgaaacaat accggcctgg tcgtacaaca ttttctcaaa
     6841 atgcgggatt ctccatttgt tctctacggt atatcttgca aaaccacctt caacatgatc
     6901 ataaatacca ccatatatta tgttgtcaag ggttatcaat gcagcatctt ttgcagggtt
     6961 gttgttgtag tagtatgcac tttgtagcag aaaatcataa ctttctgtac gtggaaattt
     7021 ctgactatca cccattctgc ctccatacac tttatcagtt ttattggtaa tggtttgtag
     7081 aatattttgg atatccgatt tagggatgat gttgccaatg acatgttcgc ctttgtgcat
     7141 ttctttaatg taatcggtta tttgagcagc atttttctgt agttcgccag gattctcggt
     7201 tttcagtgat tgaaattttt ctaaaattct aatccactga tctttaggga aataagtgcc
     7261 gacccatata ggtttgccgt cgggtagggc aatggcattc agcggccatc cacagctttc
     7321 atttgtcaac tggcaagctg tcatataaat gtcgtccacg tcggggcgtt cttcacgatc
     7381 taccttaatg cttacgaagt gatcattcat aacacgtgct actgcagtat cagagaaaca
     7441 ttccttctcc ataacatggc accagtggca agaagaataa ccgatgctga tgatgatgag
     7501 tttgtcctcc ttttgggctt tttgaagtgc ctcatcattc cacggatacc aatcaacagg
     7561 gttatgggca tgttgtagca aatatggact cgattcgttt atcaggtgat tgccggtttt
     7621 attgttatga gcatcctgtc caccggtttt acaactcaag agcagagtgc ctgcaactat
     7681 aaggaaaaga tgaagtttat ttagtaactt gtcattcatt gctaaaaata ttacaaattc
     7741 ggttaacgta ttatttttca atttgttgga agaataatca tttgaatgca ataccttttt
     7801 cctgcttaca ttaaatatga tccaacacat accttgaaca cttctctaaa acaataatac
     7861 ctcaataata attccttcta attctgcgat acattataga ctgattctga aaaacaattt
     7921 gttacttttt ggtattttaa tattgaaatg taatccatta gtcatgatta ttgtcaggtt
     7981 tatccaaaaa gtatggttct gattttcatt tcttataatt tttgtattaa ttcgcacctt
     8041 taaaataaca atcatttatt atgatcaaga aatttctatt acccattttg ttaaccagta
     8101 gttttatgct ttcagcacaa agtattcaga tcaaatatac agaatacgat ctgccaaatg
     8161 gcctgcatgt aatacttcat gaagaccaca gtacaccgat agtcgctgta tccattatgt
     8221 accatgtagg ttcaaaaaat gaaaacccaa accgtactgg ttttgctcat tttttcgaac
     8281 acctcttgtt tgaaggttcg gagaatattc ctcgtggcga atttgcaaaa atcgttgaac
     8341 gttcaggcgg aacgcttaat gccaatactt ccaatgacag aacgtattat tttgaaatca
     8401 tgccatctaa ccaactcgaa acgtcacttt ggcttgaatc tgaaagactg atgcacgcaa
     8461 aagttgataa agttggtatc gaaacgcagc gttcggttgt caaggaagaa aaacgtcaac
     8521 gtgttgacaa ccaaccttat ggtacgctca ttgaagaaat catgaagcgt atgtacacca
     8581 atcatccata caggtggatg acgatcggtt cgatggaaca ccttgatgca gcatccgaag
     8641 aagattatgt aaatttttac aagacattct acgttcctaa taatgcggta ttatctattg
     8701 caggtgatat taatgtcgaa cagaccaaaa aactgattga aaaatacttt ggaccaatac
     8761 caaagggaac aaaaccaata ttcaggccta caagcggcga tgtgccactt acatctgaga
     8821 taaaggatac agtgtatgac aatatccagt taccggccgt tatcgaagca tacagaatgc
     8881 cttctatcaa agatgaggat ttttatgctg ttagaatgtt aggccaatat ctttctgaag
     8941 gcgaaagctc caaaatgcac aaagccatag tcgatgagca acaaaaggcc gtatttgtag
     9001 gtgcatttcc gtatccgctc gaacacgaag gagtttttat cactttcggt atagctaatt
     9061 ttggtggtga tccgggtgat ttacagaaaa gtatggatga agtgatattt agcacccaga
     9121 atgaccttat ttcagaaaat gattttacta aactccgcaa tagtatcgaa actgaatttg
     9181 taagccgaaa tgctacaatg gccggaatcg ctgagacgct tgcaagtgct tatacttatt
     9241 ttaaaaatac gtcacttgtc aatacagaac ttgataaata catgaaagta acacgtgaag
     9301 acattaaacg tgtggccaac cagtatttca ataagaacaa aagagtagtg ttgtactaca
     9361 tgcctaaact caaaaaatca taaaaaccag cgtgaacatg aaaaacttaa gaatattata
     9421 tatactattt ttagtgttgg gccttggtac aacacagcta ggtgttgcac aggttaagag
     9481 caaaacaact acaaaaacac aaaccaaagc taaacctaaa gcaacaacaa ctaaaccgaa
     9541 accaatgaca gattttagaa gtcagcaacc taaggcagga ccggcaccac tcattcagat
     9601 tagtgattat accgtgcact cacttaaaaa cggtctgaaa gtaatagttg tagaaaacca
     9661 caaattaccg agagcatctt ttcagctatt tattgacaat gatccaatac ttgaaaaaga
     9721 ctcaaaaggt tacgtagatc ttgccggcca gatgcttggc aaaggaacat ccaaaaagtc
     9781 aaaggctcaa cttgacgaag caatagattt tcttggtgca tcattctctg ccaatgccaa
     9841 cggatttttt gcttcttcac ttaccaaaca ttctgaaaag ttactcgaac tagcatcaga
     9901 ggctgttctt gatcctagtt ttccggaggc tgaatttcaa aaaattaaat cgcagtcaca
     9961 gtctggtctg cagcaagcaa aagatgacgc caatactata tctgccaatg tcgcatctgt
    10021 actcaacttc ggtaaggatt tcccttacgg agaaaatatg actgaaaaaa cgcttgagaa
    10081 gataacactt caacagtgca aagaatacta caatacttat ttcaaaccaa acaatgctta
    10141 ttttattgca gtaggagata taaaggccga gaaagcaatt gcattagcag agaagttttt
    10201 tggtggctgg actcctggca ttgtacctaa aaacaattat tctgcgccaa aacagaataa
    10261 ccagccaatg gtaagctttg taaataaaga cggtgctgtt cagtctgtta tcaatgtaac
    10321 ctatccggta gtgcttaaac ctggtacaga agatgttgta agagtggcag tgatgaataa
    10381 tattcttggc ggacatggtt ttactgcccg attattccaa aatctgcgcg aaaaacatgc
    10441 cttcacttat ggagcatata gtactattgg tcccgacaaa cttgccggaa cattctctgc
    10501 atcagcatct gtcagaaatg aggtaacaga tagtgccgtt acacagtttt tgaacgaaat
    10561 gaaccggata aggacagaaa aagtgacaga cctcgaactg tcgagcatca aagcatcgct
    10621 ggcaggagat ttctcccgtt cacttgaacg accgcagaca gtagcaaact ttgcattatc
    10681 aactatacgt ttcaatctac caaaagatta ttacaaaact taccttgcca gactagatgc
    10741 cgtcaatgca gatgcagtat tgcaaacagc tcaaaaatac attttaccaa acaatgcaaa
    10801 tattcttgta gttggtaaca aagatgaagt ggcagataag cttaagcaat tcgccccaga
    10861 taaaaaggtt aatttctata ccgcttacgg agataaagta gtagatagcg caccggtaga
    10921 tttgggtaac ctgacagctg gtaatgttat tgcagattat gttaaggctg taggcggagt
    10981 tgaccagatt aacaaagtca aagatgtaca tttcattgct caaactaatg tgcagggaat
    11041 ggcaatacaa actgacaaat acgtaaaaat gccggataag tacgctgaaa aaatgtctat
    11101 gaatggttcg gtagttcagg agcaaatagt gaatggcaag aaaggtatcc agggacagat
    11161 ggggcaaaat tcagcaatgg atgaagaatc gctcaatgcg attattgatg aaattggcct
    11221 tatacctcaa gtaaaatatg aagcggaaaa atatacactt aaactgatcg gtactgaaaa
    11281 tgtagaaggt aacgatgcct acaagataga agttgtttct cctaaaggtg cgaaaaaact
    11341 tgaatactac gatgtcaaat caaaacttaa acttcgtgaa gtaatgagcc agacagcagg
    11401 agaaaaaacc gtcacaatgg taacagattt ttcagattac aaagctgttg atggtattat
    11461 gattccgcat aaagcagtca ttaccggcgg aatgatgcct gtgcctattg aaatgattac
    11521 aaactctgta gaaatcaaca agggaattaa tgattcaatg tttaaagtag attaaaattg
    11581 atatgataca ggtatatatt accggtggta catttgacaa ggagtataat ttcatcaccg
    11641 gcgaactgta cttcaaagat acacatctgc ccgacatgtt taggttgggc agatgtacac
    11701 aagacataga tgtaaagaca ctaatgatgg tcgattcgct cgagatgacg gatgatgacc
    11761 gcgaaatcat tctgcacaat tgtaagaaaa gcgaaagtga ccacatcatt atcacacatg
    11821 gtaccgatac aatggtacgt accggcgagt ttctggcaaa ggcgaatatt gacaaaacca
    11881 tcgtcatgac aggagctatg ataccctacg catttggcac tagttccgat ggatttttta
    11941 atctcggtag tgctcttgcc ttcgttcaaa cactgccaaa gggtgtgtat atcgtaatga
    12001 acggacggta ctttctctgg aataacgtaa gaaaaaataa gaaaaccggt ttttttgaag
    12061 agcttgaagc ttaacagcca aataattttc gtatgagtat ggatgcattg ggtttattgt
    12121 ttgagttaat catagctttt atcagcctag gcttattctt cctcataacc gggaagtgga
    12181 ctacctacaa cacaagatta aatgaactga tacaaagcat caacaacagt agcatgaaga
    12241 cacttttcaa atggacttca ttgatagttg gggtacttat gctcattaac tttatccttc
    12301 atgtgttgat agaaataaaa aagtaaccct atcaaacacc gtaaagtatt atttgtatta
    12361 cacatttgta gtttactgat tgttacgatt ttactctgct aattcctaag aaaatcaact
    12421 tttccggaat tattattttc ataaaatcac gtaattttta acctaaaagc atatattttt
    12481 tgtaatattg ccaattaatt tggaaaacat gagattcgtt cagttaagta ttttgttatt
    12541 attttcattt gtagcatcag ctcaaagcag tgaaaccgat attttcgatc ctaatcatat
    12601 ttctgaaatc cgccttacta taaagcagca aaattatgct gacattctgg attcacttaa
    12661 ggtgtatggt gccggaatga tagttggtga tgtgaacatt aacggtacca aaatcaagga
    12721 cgtaggcata cgctacaggg ggaattcttc atacggattt ggcaataagc gtaatccttg
    12781 gcaaataaaa ctcgactata ttaacaaaag tcaaaaatat cagggtgtca gctcaatcaa
    12841 actctcaagt gcattgcgcg acccgtcaat ggtacgtgaa gtgcttggtt tcgaaattgc
    12901 cagaaaatac atggtggcac caagagccaa ttacacaaaa ctctacgtaa atgataagta
    12961 cattggttta tatgtgaatg tggaatcaat cgacaagaat tttattgaga gaaataatgc
    13021 atcccggtcg aatacatttt ttaaatgcag tcctgatcct gacaccaaat ctaacccggg
    13081 ctgcaagaac aaaatttatt ctgcactagt atatgagcaa gacccaaatt gttttctacc
    13141 aaattataca ttaatttctg agtcaggttg ggatgaactc attgaactga ctaaagtact
    13201 cgagcaaagc cctgctaata ttaatagtat
//
LOCUS       DAOQQY010000047        13100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1614_length_13100_cov_5.646378, whole genome shotgun sequence.
ACCESSION   DAOQQY010000047 DAOQQY010000000
VERSION     DAOQQY010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1614_length_13100_cov_5.646378"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="PLE23_04075"
     CDS             <1..795
                     /locus_tag="PLE23_04075"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMX82474.1"
                     /translation="VHNDYLDIDAAVATRQFENAYFLIDDNKQFSCSWEVEKMSKSKY
                     NVVNPDDMVAAYGADCFRMYEMFLGPIDQAKPWDTKGIDGVAKFLRRLWSLFFDENGK
                     IQLSDTEPTREELKILHQCIKKVSEEVERFSFNTCVSAFMICVNDLRRIECRNLSVLK
                     DLLKLVSPFAPHIAEELWEQSGEAFSVTLQPYPVFDEKYLKEDHIEYPVCINGKKRAL
                     LLLPADMDKADAEKQAVSLPEITKWLDGTPVKKVIVVSGKMINIVI"
     gene            896..6640
                     /locus_tag="PLE23_04080"
     CDS             896..6640
                     /locus_tag="PLE23_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HMX82475.1"
                     /translation="MKLPISLLTCLSLIISALNAHAQNPLVCDFRPTALQAGNITVNA
                     ALLSWVDPNNASEWEIEIRRKNESFSSPNYTVSSNPYLMTGLESGVEYKYRVRSKCIG
                     GSVSNWSINPYLFVTVMTNPSTCLMKLDIPDNSCALGHKNFPIEVKNVAGNSLGTNVI
                     LKEVRMIMAHTWLRDLDVLLVSPNNKVVRLTKENGGSNDNYGDPTDLTCVRSTVFTET
                     ICTAPSIKEGSAPYIGSYYPEESFSKFYDGSNPNGNWTLRVCDGAGGDIGTIESLELI
                     FLPSNCTAPYDLSYFGENYNSVFVEWKSNGPCIKTIIEVGPKGFTPGNNYAAGAQGTV
                     FAVDCPLTGPFQITGLSELTDYDVYVRKDCGGGNFSVNSCPISFKTECNTHNPVTISE
                     SFDSQPLCNTCSCGEGSSVFGVFENNKEGDFDWLVREGPTPSANLLTGPDGDVDDSGR
                     YLYLETSGSSCQNKQAILQSGCIKVSASSQDGCHMSFYYHMRGATINKLSVFITENGG
                     ATWQEVWSATGAQGKKWRRAYIDLAQWNTKTVQVRFVGQSGNGDTGDIAIDQIEFYGS
                     TYQGQPGNLYFADNDGDGYGNALSTIKTCDAVPPAGYTTNNADCDDNNELSYPGAPEI
                     LCNNTDENCNGMADDVILTNPIVSGQNTCENSSDTLTVITPLTGNAYWFSSPTAKQPI
                     FVGNPFVTPPLNANTIYYVMDSSSTFGCYSQRVPVSVFVYKVPLLVTNDQPKICRGTS
                     FDLATLNITDLKNAGGVLSYHNGFPATNGNKLNNTIVSPLSSATYYVRSVNANGCEGV
                     ISVLLKVNPVPDIQIQNPDSLAICSSAQLTLVSIGTGGTFPYTYKWSNGSPDYTTIIS
                     GSPSPGTIQYSVTITDANNCTDMDNITVINKPGVAGAGFVASDVTTCLGDNGSIFVNP
                     VGNGPFNYFWSGPVSGSYTNIPGSVTIMNLKKGSYSVTVTQTGNECGYILPVVVINGP
                     GVQLLSSKVTDVSCAGLADGSIELEVSGSNLSFQWNSGETSKNISNKVPGFYSVHISD
                     GICDLDINNIQIKAPTTLAVSGVPTAPACFGINSGKIELYITGGTTPYSFKWSNGDTT
                     QNITDINAGNFVCTVTDAHGCSVVTPVYTLSDPATLAVFSIKDNVDCFQGTDGVISVS
                     VLGGVPPYSYSWNDGVKAKDRNLLAAGTYKLTVTDQKGCSVTSSNIEITQPPKLQAFV
                     SSLEQTSCKNLSNGQISINVTGGTTPYEYLWSNNFQGKDLLNASQGNYSITVTDANNC
                     RTSIIGMKISSADSILIALQKLTNTTCNTIANGSIVMNVSGGNGNYQYQWSNGATTKN
                     LASIPSGTYQLTVSDSGGCAIVSDIYTVSEISPVSIILENLEIADCASNQKGNIDISA
                     SGQAPFSFKWSNGATTEDLYGVFNGMYGVTVTDNQGCKATYNNINLSGTGDPFFVHLN
                     VLEGISCPGSKDGRLVVHIDGGVAPYQYNWSTGKEYDLEAQTDSVSGLFAGNYAITIT
                     DNRGCVVSDTVVLSAPAALTLNVKNIKNLACKGKNTGAILLEVSGGTKPYKFVWTTPD
                     GTIENTTANFTGLKGGLYSVMVIDANGCTQELPAPVMLSEPPVAFQFENVFVTQPGCS
                     GENTGSVSVFTKGGVGMTQYSWNPPSLVGSFATNLTGGTYKITVTDQNNCSIDTTIIL
                     EPYKPMNVTASVNSDPVCNSNKNYIFLSVSQGKAPYTYKWNTGSSSGFIDSLFAGTYW
                     VQVSDAKGCIFNDTFAVGVQGMVLDSVISKPNTGPLPNGSAAVYVSGGKPPYKYKWEE
                     KAGSGTTSFVSNLSSGYYCLLVEDQNGCKMTVCVEIENKTSTADIPDTGSGADIYPNP
                     ANSTIQIRDISKLNGKEHIYIFDALSRIVQNKDIEWREGISSEIDISHLPSGIYWLVY
                     KNKAQNRYLKFIKL"
     gene            complement(6666..8618)
                     /locus_tag="PLE23_04085"
     CDS             complement(6666..8618)
                     /locus_tag="PLE23_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82476.1"
                     /translation="MYQTFFVFIIAVLSCSAPAIVRDGKTAFERKQYNLAASLLEKEY
                     TKSDTRVKKGKIAWLVGLSLKNTGESAQSVKWFKTAYDNQFGVEALREYAFALKQTEQ
                     YKEAESAFRTLGQEIGSPFEYRKEITACQSAARWLALKDDNGYKISPASFNTAQNEYA
                     PYISKDGDIYFTADKQANSKQDKYYWTGRGFSDIFILKKNDTKPEKLNTNINTANNEG
                     TISISPDGKTMIFTRCDGEKNIDRYCKLMQSEYVDGQWSTAVQLEFQQERINYMHPAF
                     SADGNTLFFASNDPNGIGGYDVYFTQKSKGSWLEPQILPNNINTAGNEKFPYLDADTL
                     YFASDGWQGMGGLDIFKVSKSGTNTWNQPINLQAPMNSGSDDFGLTVDPGFMPNENVF
                     KKGYFSSNRPGGSGKDDIYEFRLVKVEPKPKAAPPGDLYLDVYVLEKIRKDPADPNST
                     VIARKPIKNAKLTVLNLEGKKDYNTSADEAVSIKLNPGAQYQFTAGADGFLTGTSVFS
                     AVGISPIPGQDQRFELEIVLDKIFKNKEIILENIYYDFDKWDIRQDAMPTLNKLAGLL
                     KSNPDIKIQLGSHTDCRGGTAYNQILSQKRAQSVVDYLIAQQIAPERLTAVGFGESKP
                     AVNCECSKCSEDEYQTNRRTTFTILE"
     gene            complement(8982..12047)
                     /gene="secDF"
                     /locus_tag="PLE23_04090"
     CDS             complement(8982..12047)
                     /gene="secDF"
                     /locus_tag="PLE23_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HMX82477.1"
                     /translation="MQGKGIIKFFLILLTAVCLMQYLFVLPTRGIEKAADEYAAKMAA
                     KAVPDQKATVERQARTSYLDSMSSEVVMKVPLLKDYTYEDLKKQQLALGLDLKGGMSV
                     ILQVDLKDFVHSLSNESKDPILLKALDNTEKIQEKTPGDFVSFFASEFTKAGGTSLAS
                     IFGRNEALKDKINLSSPNSAVIAVLRAKATETVDLTFKRLKDRIDKFGVTQPNVSLDA
                     ARDLIIVELPGIDNPERARRFLQASAKLEFWDVYRTSDNGISQAMQAANSKLEAILGG
                     KTGEAVSDTTNAAAVPDSSQNALLAAVDTSASKLSSGKGPLFSIFTPNLSTGQQMTAA
                     PSVMGFADKNKMELISSYLNRPEIKTMFPADLEFRWSAKPTKDNTTKKATKTYELYAI
                     KKVKGDKAPLEGDHVTDASANPDPRSGAVAVSLKMDSRGAKIWGDMTTKAAQGGNREI
                     AIVLDDEVVSAPRVQNAILTGDSQITGDFTIQDGKDLANILQIGKLPAKTKIIQESLV
                     GPSLGADNINKSLKSIALGFLALLAFMAIYYSTAGFVSIAALFANFILIIGALSNLGT
                     VLTLPGIAGLVLTMGIALDINVVIYERIREELRMGKSLATSIKEGFKMSLSAIIDANV
                     TTLLSASVMAYFGLGPIKGFAVVLIIGVLFSMITAIIVTRFLIDWLVRKGKDVHFWIP
                     ATKNSLTGVNIDWIGIRKKTYLISAIIIITTIISIFTRGFELGVDFKGGYSYNVSFDK
                     NAKIDVEKLRQNLTSAFDGATPIVKQVDVANTFNITTSYLIDKPGEGVDEQVVQKLYN
                     AIKATTGTNESIDDFKNADYTGTKLVSSSKVGATVADDISRSSFKAAFFALLLIFIYI
                     LFRFNKWQYSAGAVLGLMHDAVVMLGTFSLLHGFLPFSMEVDQAYVAALLTIIGYSNN
                     DTVIVFDRIREFLHIHKDKPDHEVINMAINSTLSRTIVTSLLTLLVVLVLFLFGGSSV
                     KGFAFALLIGIVAGTYSSIFVASPIAYDLSKSMKIETKAEKKHFSRSIK"
     gene            complement(12220..12927)
                     /locus_tag="PLE23_04095"
     CDS             complement(12220..12927)
                     /locus_tag="PLE23_04095"
                     /inference="COORDINATES: protein motif:HMM:NF015866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HMX82478.1"
                     /translation="MITLEICAGSLVSAIAAAQGKASRIELCSALTVGGLTPGVGLLQ
                     TVKSLIDIPVHVLIRPRAGNFTYSKHEKRCILREIEACIKAGADGIVIGALNADSSID
                     TDFMKEAVYCAAELDCTFHRAFDLITDKTKALDEIINLGFDRILTSGGQQSAWDGREI
                     IRQLIVHAADTIEIMPGSGIHVGNAAQIVEYTGARAIHTSAKGSNSATDVSMFAAEPE
                     TDAVSVRNIIDNLEKFS"
BASE COUNT         3688 a         2688 c         2777 g         3947 t
ORIGIN      
        1 gtacacaatg attatctcga tattgacgct gctgtagcta caagacagtt tgaaaatgct
       61 tacttcctaa tcgatgataa caagcagttc agctgttcgt gggaagtgga gaaaatgtcg
      121 aagtcgaaat acaacgtagt aaacccagat gacatggttg ctgcctatgg ggcagattgt
      181 ttcagaatgt acgagatgtt cttgggccct atcgatcaag caaagccatg ggataccaaa
      241 ggaatagacg gtgtagctaa atttttgaga agattgtgga gtttgttttt tgatgaaaat
      301 ggcaaaattc agctttctga taccgaacca acacgcgaag agttgaaaat tcttcaccag
      361 tgtatcaaaa aagtaagcga agaagtagag cgtttttcct tcaatacatg tgtgagtgct
      421 ttcatgatat gtgtaaatga tttaagacgt atagaatgcc gcaatttgtc ggttcttaaa
      481 gatctgctca aactagtatc accatttgct ccacacatag cagaagaact ttgggaacaa
      541 tcgggtgaag cattttctgt taccctacag ccttatccgg tatttgatga aaaatacctc
      601 aaggaagacc acatcgaata tcctgtatgt ataaatggca aaaaacgtgc actccttctt
      661 ttgcctgctg acatggataa agctgatgct gaaaaacaag ctgtttctct tcctgaaatt
      721 acaaaatggc tcgatggcac tccggttaaa aaagtcatcg tagtgtcggg caaaatgata
      781 aacatagtca tttagaaaaa taacattgca tttttgaagt attatttata acttgtcgtt
      841 caattaacaa gcactttttc tttttaggac gaatttaaac gaaaccatga atcgcgtgaa
      901 actcccaata tcactgctta cctgcttatc tttgattata agtgccttaa atgcccatgc
      961 acaaaatcca ctcgtgtgtg acttcaggcc tacagcatta caggctggta atattactgt
     1021 caacgctgcc ttactgtcat gggttgaccc gaataatgcc agcgaatggg agattgaaat
     1081 tcgccgaaaa aatgaatcat tcagctcacc taactataca gtttcaagca acccgtatct
     1141 gatgacaggc ctggaatcgg gtgtagaata caaataccgg gtacgctcta agtgtattgg
     1201 tggttctgta agtaattggt ccatcaaccc gtacttattt gtcaccgtta tgaccaatcc
     1261 atctacctgc ctcatgaagc tggacatacc ggataacagt tgtgcacttg gacacaagaa
     1321 ttttccgatt gaagtaaaaa atgtggcagg taattctctt ggtacgaatg tcatcctaaa
     1381 ggaagttcgt atgataatgg cacacacctg gctacgtgac cttgacgtgt tgcttgtttc
     1441 tcccaataat aaagtggtta ggctaacaaa ggaaaatggt ggcagtaatg ataattatgg
     1501 cgacccaact gaccttacat gtgtgcgttc tacagtattt acagaaacca tctgcacagc
     1561 gccttctatc aaggaaggtt ctgctcctta cataggcagt tattatcctg aggaatcatt
     1621 cagcaaattt tatgacggat cgaacccaaa cggcaattgg acgctgcgtg tatgtgacgg
     1681 tgcaggtggc gatatcggta ctattgaatc gcttgaactc atttttctgc cttccaattg
     1741 tactgcacct tatgatctga gttattttgg tgaaaattat aattcggttt ttgtggaatg
     1801 gaaatcaaac ggaccttgca tcaaaacaat cattgaagta gggccgaaag gattcactcc
     1861 gggcaataat tatgcagccg gggcacaggg cacagtcttt gccgttgatt gtccgcttac
     1921 agggccgttt cagataacag gcctcagcga acttacggat tatgatgtgt atgtcaggaa
     1981 agactgtggt ggcggaaatt tttcggtaaa ttcctgcccg atttcattca agaccgaatg
     2041 caatactcat aatccggtaa ctatttcaga atcatttgat agccaaccac tttgtaatac
     2101 ctgtagttgt ggtgagggaa gttctgtatt cggagtattt gaaaataata aggaaggtga
     2161 tttcgactgg ttggttcgag aagggccaac accatctgct aacttactca ctggtccgga
     2221 tggcgacgtg gatgattcag gccgttactt gtatcttgaa acatcaggtt cgtcttgtca
     2281 gaataaacag gccattctgc agtcgggttg tatcaaagta tcagccagta gtcaggatgg
     2341 ttgccacatg tcgttttatt atcacatgcg tggagcaacc atcaacaaac tttcggtttt
     2401 cataacagaa aacggtgggg ccacatggca ggaagtttgg tcagcaacag gtgcccaagg
     2461 caagaagtgg cgaagagcat acattgacct ggcgcaatgg aatactaaaa ccgttcaggt
     2521 gaggtttgta gggcagagcg gcaatggtga tacaggtgat atagccattg atcagataga
     2581 attttacggc tctacctatc aggggcagcc cggtaatcta tactttgccg ataatgatgg
     2641 tgacggctac ggtaatgcac tctctacaat caaaacatgt gatgccgtgc ctcctgccgg
     2701 ctatacaact aataatgccg actgcgatga caataatgaa ctgagctatc cgggggctcc
     2761 ggagatattg tgcaacaata cggatgaaaa ctgcaatgga atggcggatg atgtcattct
     2821 taccaatcct atagtttccg ggcaaaatac atgtgaaaac tcgagcgata ctttaaccgt
     2881 aataacacct ttgacaggca atgcatattg gtttagcagc ccaacagcca aacaaccgat
     2941 ctttgttggg aatccatttg tcacaccacc gctcaacgcc aatacaattt attacgtcat
     3001 ggatagctca tcgacttttg gctgttatag ccaacgtgta ccagtgagtg tttttgtata
     3061 taaagtgccg ttactggtaa caaatgatca gccaaaaata tgtcgtggca ctagctttga
     3121 tcttgctaca ctcaatatta cagatctaaa gaatgctggt ggagttttga gctaccataa
     3181 tggttttccg gctacaaatg gcaataaact gaacaataca attgtgtcgc cgctatcgtc
     3241 tgctacgtat tatgtgcgtt ctgtcaacgc caatggctgc gaaggagtga tttctgtatt
     3301 gctgaaggta aatccggtac cggatattca gattcagaat ccggattcac tagcaatctg
     3361 ctcctcagcc caattaacac ttgtgtccat cggtacaggc ggtacatttc catacactta
     3421 taaatggagt aatggttcac cggattatac taccattatt tcgggctcac caagtccggg
     3481 taccatacaa tattcggtga ctattacaga tgcgaataac tgcacagaca tggataatat
     3541 aacggtcatc aataaaccgg gcgtagcggg tgcaggtttt gttgcttctg atgtcactac
     3601 ttgtctgggt gacaatggta gtatttttgt gaatccggtt gggaacggtc cgttcaatta
     3661 tttctggtct ggtccggttt ctggctctta taccaacatc ccgggcagtg tcaccatcat
     3721 gaatctcaaa aaagggagtt atagtgttac ggtcacacag actggcaatg aatgcggcta
     3781 tatcttaccg gtagtggtca tcaatggtcc gggtgtgcag ttgctgagta gcaaggtgac
     3841 tgatgtatcg tgtgccgggt tggcagacgg ttctatcgaa ctcgaagtgt ctggctccaa
     3901 tctgagtttt cagtggaact cgggcgaaac aagtaagaac ataagtaaca aagtgcccgg
     3961 cttctatagt gtacatatat ctgatggtat ctgcgatttg gatattaata atattcagat
     4021 aaaagctcct acaacgctgg cagtatctgg ggttcctacc gcaccggcat gttttggtat
     4081 caattcaggt aaaatcgaac tttacattac cggaggtacc acaccttatt ccttcaaatg
     4141 gtcaaatggt gatactactc aaaatattac cgacatcaat gcagggaatt ttgtctgtac
     4201 ggttaccgat gcacatggtt gttctgtagt tacacccgta tatacactca gtgacccggc
     4261 tacacttgca gttttttcaa taaaggataa tgtagattgc ttccaaggta ctgatggagt
     4321 gatctcggta tcagtattag gtggagtgcc gccttattct tacagttgga atgatggtgt
     4381 caaagctaaa gaccgaaatt tacttgctgc aggtacctac aaacttactg tcacagatca
     4441 aaaaggatgt tctgtaactt cttctaatat tgaaataacc caaccgccca agcttcaggc
     4501 atttgtaagt tcacttgaac agacttcctg caaaaaccta tccaacggtc aaataagtat
     4561 taatgtaaca ggtggtacaa ctccgtacga gtatctgtgg agcaataatt ttcagggtaa
     4621 agatttatta aacgcatcgc agggcaatta cagcattact gtcactgatg ccaataattg
     4681 ccgaacctcg atcataggca tgaaaatttc aagcgcagat tcaattctta tcgcacttca
     4741 aaaactcacc aatacaacat gtaatactat tgcaaacggt tcaattgtta tgaatgtaag
     4801 tggaggcaat ggcaattacc agtatcagtg gagcaatggg gcaaccacaa aaaatctagc
     4861 ttctattcct tctggcacat accagcttac cgtttctgat tcaggcgggt gtgcaatagt
     4921 gtcggatata tatactgtca gcgaaatatc gcctgtcagc atcattctgg aaaatcttga
     4981 aatagctgat tgcgcatcga accagaaagg taatatcgac atctcagcat ccggtcaagc
     5041 tccttttagc ttcaaatgga gcaatggagc tactactgaa gacctctatg gtgtgtttaa
     5101 cggaatgtat ggtgtgaccg tcacggacaa tcagggttgc aaggctactt ataataatat
     5161 caatctgtcg ggtacgggtg atccgttttt tgtacatctt aatgttcttg aaggtatatc
     5221 ctgtccgggg agcaaggacg gcagacttgt ggtgcacatt gacggaggag ttgcaccata
     5281 tcagtacaac tggagtaccg gtaaggagta tgatcttgaa gcacaaaccg attcagtctc
     5341 aggattgttc gcgggcaatt atgccattac tatcacagat aatagaggtt gcgtagtaag
     5401 tgatacagta gtattgagtg ctcccgcagc tttgacactc aatgttaaga acattaaaaa
     5461 cctcgcttgc aaaggtaaga acaccggtgc tatactgttg gaagtatccg gaggaacaaa
     5521 accctacaaa tttgtctgga ctacacccga tggtacgatc gaaaatacaa ctgctaactt
     5581 tacaggactc aaaggtggcc tatattcagt catggtcatt gatgctaatg ggtgtacaca
     5641 ggaattaccc gcaccggtta tgctttccga acctcctgtc gcatttcagt tcgagaatgt
     5701 gtttgttacc caaccaggct gctcaggaga aaataccggc tctgtatcgg tgttcaccaa
     5761 aggcggtgta ggcatgaccc aatattcctg gaacccgcct tcgctggtag gttcatttgc
     5821 tacaaacctt acaggtggca catataagat tactgttaca gaccagaaca attgttcgat
     5881 agacacgacc attattctgg agccatacaa accaatgaat gtaacggctt ctgtcaacag
     5941 cgatcctgtt tgtaacagca ataagaatta cattttcctt tctgtaagcc aaggcaaagc
     6001 cccttataca tacaaatgga acaccggctc ttcatcaggt tttattgatt cgctctttgc
     6061 cggtacatac tgggtgcagg tgtctgatgc caaaggttgc atattcaatg atacctttgc
     6121 agttggtgta caaggtatgg tactcgattc ggtcatttca aagcccaaca ccgggccatt
     6181 acccaacggg tctgcagcag tgtatgtaag cggtggtaag ccaccatata agtacaaatg
     6241 ggaagaaaag gcaggaagtg gcactacttc ttttgtaagt aatctgagct ccggttatta
     6301 ttgtctgttg gtagaagacc aaaatggttg caaaatgaca gtatgtgttg aaatagaaaa
     6361 caaaacaagc actgcagaca ttccggatac cggatcaggg gcagatattt acccaaatcc
     6421 ggctaacagt acaatacaga tacgggatat atccaagctc aatggtaaag aacatatata
     6481 tattttcgat gctttgtcaa ggattgtaca gaacaaagat attgaatggc gagagggaat
     6541 ttcttccgaa atagatatat cacatttgcc ttctggtatt tactggttgg tttataaaaa
     6601 taaagcacaa aacaggtatt tgaagtttat aaaactctga gaaatatgaa ccaaacttag
     6661 agcttttatt caagaatggt aaaagtagtt cttctgtttg tctggtattc gtcctcacta
     6721 catttactac attcgcagtt gacggccggc ttcgattccc caaacccaac cgctgtgaga
     6781 cgctctggtg caatctgctg tgcaatcaaa taatcaacta ccgactgagc ccgtttctga
     6841 gacagaatct gattgtatgc tgtgccacca cggcagtctg tatgtgaacc caactgtatt
     6901 ttgatatccg ggttactttt gagcagcccc gcaagtttgt tcaatgttgg catggcatct
     6961 tgccgtatgt cccatttgtc gaaatcgtag taaatattct ccagtatgat ttctttattt
     7021 ttgaaaattt tatccagtac gatctctaac tcaaaccgct gatcttgtcc gggaatcgga
     7081 ctgatgccta cagctgaaaa tacggatgta cctgtaagaa aaccatcagc tccggctgta
     7141 aattggtatt gtgcaccagg atttaactta atgctcacag cttcatctgc agaagtgtta
     7201 taatccttct ttccttccaa attcagtaca gttaattttg catttttgat tggttttcgt
     7261 gcaataaccg tactattcgg gtctgccgga tcttttcgta ttttttcaag tacgtacaca
     7321 tccaagtaca aatcgccggg aggggcagcc tttggttttg gttcgacttt aacaagcctg
     7381 aactcataaa tatcgtcttt gccgcttcct ccgggtctgt tggagctaaa atatcctttt
     7441 ttaaacacat tttcatttgg catgaaacca gggtccactg tcaatccgaa atcgtcggag
     7501 ccactattca taggtgcctg taggtttatg ggttgattcc aggtgtttgt accagatttg
     7561 gaaaccttaa aaatatctaa accgcccatg ccttgccatc cgtcggaggc aaaatacaaa
     7621 gtgtctgcat ctaagtatgg gaatttttca ttaccggcag tattgatatt gtttggcaat
     7681 atttgaggtt caagccagct accttttgac ttttgagtaa agtacacatc atatcctccg
     7741 ataccgtttg ggtcatttga agcaaaaaaa agcgtattgc cgtctgccga aaatgccgga
     7801 tgcatataat taattctttc ctgctgaaac tcaagctgta ccgcagttga ccattgtccg
     7861 tccacatatt cgctctgcat cagcttgcag tatcggtcta tatttttttc accatcacat
     7921 ctggtgaata tcattgtttt tccgtctggg ctaatactga ttgtcccttc attgttagcg
     7981 gtattaatgt tggtattcag cttttctggt tttgtatcat tttttttcag aataaaaata
     8041 tcagaaaaac ctcttcctgt ccagtagtat ttgtcttgtt ttgagtttgc ttgtttgtcg
     8101 gcagtaaaat agatatcgcc gtctttgcta atatatggcg catattcatt ttgagctgtg
     8161 ttgaatgagg ccggacttat cttgtatccg ttatcatctt ttaatgcaag ccatcttgca
     8221 gcagattggc aggcggttat ttctttacga tactcgaaag gactgccaat ttcctgccca
     8281 agtgtgcgaa atgccgattc tgcttctttg tactgttctg tttgcttgag tgcaaaggca
     8341 tattcccgaa gggcttcaac accaaattga ttgtcatagg cagtcttaaa ccatttgacc
     8401 gactgagcgg attctcctgt gtttttgagc gagagcccta ccaaccatgc tatttttcct
     8461 tttttgacac gagtatcact tttggtatat tctttttcca gcagagaggc agccagatta
     8521 tattgttttc tttcgaatgc agtctttcca tcacgcacta tagctggtgc cgaacaggac
     8581 aacactgcta taatgaagac aaaaaaagtc tgatacatta gcttattcat aaatattcaa
     8641 tgacgagtta tgtcaataat ctacataacg cttgaaacgg gttttaatcg tataaaaatg
     8701 cttcgttatg taatttcttg tattgcactg cgggcttaca tttgtaaaat tgttaataac
     8761 taaagcgaat atagcattta ttcatcaaat ttttattttg tttgatcaga tgtacgttgt
     8821 ggtttgggtt ttgatttttc gtagttgaaa gggaaggcgg tatttattca aattgacttt
     8881 tacttgtctt ttttttaatt tggaaattgc ggagcgacac aaaagaaaaa cgcctcggct
     8941 aatataacca aggcgttgat ctgaagatat tttgattaga attatttaat ggatcttgag
     9001 aaatgttttt tctctgcttt ggtttctatc ttcattgatt ttgacaaatc gtatgcaatc
     9061 ggtgaagcaa caaagatgga agaataggta cctgcaacga taccaatcaa caatgcaaaa
     9121 gcaaaacctt taacgcttga accaccaaac aagaagagta ccaatactac aagcaaggta
     9181 agcaacgatg tgacaatggt acgactcagc gtactgttga tagccatatt gattacctcg
     9241 tggtcaggtt tgtctttatg gatatggagg aattctctga ttctgtcaaa tacaattacc
     9301 gtatcattat tcgagtaacc aatgatggta agcaatgctg ctacataagc ctggtcaacc
     9361 tccattgaga aaggaaggaa tccatgaagc aatgagaaag tacccaacat aacaacagca
     9421 tcgtgcataa gacctaatac tgcaccggcc gagtattgcc atttgttgaa tctgaataat
     9481 atgtaaatga atatgagtaa taaagcaaag aatgcagcct taaatgacga acggctaatg
     9541 tcgtctgcca ctgtggcgcc caccttgctc gaacttacta attttgtacc ggtataatct
     9601 gcatttttaa agtcgtcgat gctctcattg gtaccggtag ttgctttaat ggcattatac
     9661 agtttctgaa caacctgctc atcaacacct tcacccggct tgtctataag gtaactggta
     9721 gtaatgttaa atgtattggc aacgtccacc tgctttacga ttggtgttgc gccatcaaag
     9781 gcacttgtta ggttttgtct gagtttttct acatcaatct ttgcattttt gtcaaacgaa
     9841 acattgtatg agtatccacc tttgaagtca actcccaact cgaatccgcg tgtgaatata
     9901 gagatgatag tagtaataat gatgatagct gagattaggt atgttttctt tctaatgcct
     9961 atccagtcga tgttgacacc ggtaagcgaa ttcttagtag ccggaatcca gaaatgtaca
    10021 tccttgccct tgcgaaccaa ccagtcgata aggaatctgg tgacgatgat ggctgtaatc
    10081 atggagaata gtacacctat aataagcact actgcaaagc ctttgatagg gccaagtccg
    10141 aaataggcca ttacggatgc agataataat gtagttacgt tggcgtcaat aatggcagac
    10201 aatgacatct tgaaaccttc cttaatagaa gtagcaaggc tcttgcccat acgaagctct
    10261 tcgcggatac gttcatagat aactacgttg atgtcgagtg caatacccat tgtaagtaca
    10321 agaccggcaa tacctggaag tgttagtact gttccaaggt tggagagtgc cccgatgatg
    10381 agtataaagt tggcgaacaa agctgcaatc gatacaaaac cagcagtact ataatatata
    10441 gccatgaatg caagcaatgc aaggaaacca agggctattg acttaagcga cttgttgatg
    10501 ttatcagcac caagcgacgg accaactagc gattcctgga tgatttttgt ttttgcaggt
    10561 agtttaccaa tttgtaatat attggcaagg tctttaccgt cctggatggt gaaatcacct
    10621 gttatctgcg aatcgcctgt gaggattgca ttctgtacac gtggtgcaga tactacttca
    10681 tcatccagta cgatggctat ttctctgtta ccgccctgtg ctgctttagt ggtcatgtca
    10741 ccccatattt tagcaccacg gctatccatt ttgagcgata ctgctacagc acccgaacgt
    10801 gggtcagggt tggcacttgc atctgttaca tggtcacctt caagtggtgc tttgtcacct
    10861 tttactttct tgatggcata aagttcgtat gttttagtag ctttctttgt agtattgtct
    10921 tttgtaggtt tagctgacca tctaaactca aggtcggcag ggaacattgt ctttatttcc
    10981 ggacgattga ggtaagagct tatcagttcc attttatttt tatcggcaaa acccataacc
    11041 gatggtgcag ctgtcatttg ctgaccagta gaaaggttag gtgtaaatat actgaacaac
    11101 ggtcctttgc ccgagctcaa tttgctcgca cttgtgtcaa cagctgccag taaggcattc
    11161 tgcgaactat caggaacagc ggcagcgttt gtggtgtcag atacagcctc tcctgttttt
    11221 ccaccaagta tggcttccag tttcgaatta gctgcttgca tagcttgcga aataccgttg
    11281 tcggaagtgc ggtaaacgtc ccagaactct agcttggcac ttgcctgaag gaatcttctg
    11341 gctcgctcag ggttgtcgat tcccggaagt tcgacaatga taaggtccct agctgcatca
    11401 agtgatacgt taggctgcgt taccccaaat ttatcaatac ggtctttgag tctcttgaat
    11461 gtcaggtcaa ccgtttcggt tgcttttgcc cttaagacgg ctatgacagc actgttaggt
    11521 gagctaaggt tgatcttgtc tttcaatgct tcattgcgac caaaaattga tgcaaggctt
    11581 gtaccacctg cttttgtaaa ttcggatgca aaaaaggata caaaatcacc aggtgttttt
    11641 tcctgaattt tttctgtatt atctaatgct ttcaaaagta ttgggtcttt tgattcatta
    11701 ctgagtgaat gtacaaaatc ctttaggtca acctgtagta tcacactcat accgcctttg
    11761 aggtcgagcc cgagtgctaa ctgctgtttt ttcagatctt cataggtgta gtctttcaaa
    11821 agaggaactt tcattaccac ttcacttgac atggagtcga gataagacgt tctggcttgt
    11881 ctttcaactg tggctttctg gtcaggtaca gcttttgcag ccatttttgc agcatattca
    11941 tccgctgcct tttcaatgcc tcttgtcggt agtacaaata aatattgcat cagacataca
    12001 gctgtcagta aaatcagaaa gaacttaata attcctttac cttgcatggt ataaatttaa
    12061 atttttcaaa tatgtgtgta aaaaactgcg caaatataga aatattactg aaaaaatcct
    12121 catttgtttc attacttatg caataaatat tatttggcgt cattccgaat gcaaacttta
    12181 ttccaattta tacggcagtg cttaataatc aggcagatgt tatgaaaatt tttcaagatt
    12241 atctataata tttcttactg acactgcatc tgtttcgggc tcggctgcga acatactgac
    12301 atctgtggct gaattacttc cttttgcact cgtatgtata gcccgggctc cggtgtattc
    12361 cacaatttgt gctgcgttgc cgacatgaat accacttccg ggcattatct ctatagtatc
    12421 cgctgcatga actatcaatt gtcgtatgat ttcgcggcca tcccaggcac tctgttgtcc
    12481 acctgaagtc aatattctgt cgaatccaag gtttataatt tcatcaagcg cttttgtttt
    12541 atcggtaata aggtcaaatg cacggtggaa cgtacaatca agttctgcag cacaatacac
    12601 agcttctttc atgaaatcag tatctatgga tgagtcggcg ttaagagcgc ctataactat
    12661 accatccgct ccggctttta tacaagcttc tatctctcgt aatatacagc gtttttcatg
    12721 cttagaatac gtaaagttac cagcacgtgg gcgtataagt acgtgtacag gtatatcaat
    12781 aagcgatttg actgtttgta gaagccctac accaggtgtt agaccaccaa cagtgagagc
    12841 actgcagagt tctattcgcg aagctttgcc ttgggctgct gctattgccg aaaccagact
    12901 gccggcacaa atttcaagtg ttatcatccg gtgtgttcag ttttatgggc caaataagta
    12961 tggataaccg atttgatatt tttataaatt tttttctttt tcagtgcatt atcttcagta
    13021 atccattcca gtttttcgat tccttcaccg acctccggta cgagtttgcc acatttgtag
    13081 gtcatcacat accagtatgt
//
LOCUS       DAOQQY010000048        13053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1623_length_13053_cov_6.596784, whole genome shotgun sequence.
ACCESSION   DAOQQY010000048 DAOQQY010000000
VERSION     DAOQQY010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1623_length_13053_cov_6.596784"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="PLE23_04100"
     CDS             complement(<1..403)
                     /locus_tag="PLE23_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HMX82479.1"
                     /translation="MSGKSIVIIGAGLSGTLLAIRLAQRGFKVQLHEKRADMRHVQMS
                     AGRSINMALSDRGIKALKLIGMDEFMLNASIPMTGRMIHSPDGQTSLQPYSGRPGEYI
                     NSISRGGLNIGLLDKAESYENISLYFNSDCID"
     gene            522..767
                     /locus_tag="PLE23_04105"
     CDS             522..767
                     /locus_tag="PLE23_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82480.1"
                     /translation="MSPKTWNIWLKPFNFDKKIDMSQTTQYKSSIGWVGSLVFLLISL
                     WLLVDATHSKSDFRTFFAGFLVLFFCLTLFYTLSDKD"
     gene            complement(850..1581)
                     /locus_tag="PLE23_04110"
     CDS             complement(850..1581)
                     /locus_tag="PLE23_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82481.1"
                     /translation="MKKLWVLLFICVSYTAQSQVWFEAAAKGTYGFKGFLNSNIMDDG
                     RVSYDLPTGYAYGGRFGVNFGYHNGISVEALLADNKQTFGVEGPLGIRQDYLINWKAT
                     DLYLLYRLYGDKTFLELGPKLSRLSALKQTITTGSLVDELTLEKGKDYVDYWSAALGF
                     GTYVFGAANFSMMLGARFEYAFQDLVGEQGKNRKLPLPTLVPQYSNYKSSNPYSIQVC
                     LEFNFGLGYFAKAACGRRGFFFKYD"
     gene            1748..2677
                     /locus_tag="PLE23_04115"
     CDS             1748..2677
                     /locus_tag="PLE23_04115"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMX82482.1"
                     /translation="MKFKTPIPVSEISRMIEAEMLGNTQQMATGINEIHKVTPGDIAF
                     VDVVKYFKKTINSAATIIILNEKTECPPGKTLLLVDEPFTAYNYLVRQFMPFEAMVNS
                     VSHLADIHPDAIIEPGAIIGQHVSIGVGTIIQANAVICSHSIVGKNCIIQPGAVIGSD
                     AFYFKKNKQIYHKWNSCGRVIIEDDVYIGANCTINRGVSGDTIIGAGSKIDCLVHIGH
                     GVVIGKNCLIAAQTGISGKTTLEDNVVLYGQVGIAQNINIGAGAVISAKSGISKDLDG
                     GKTYFGVPASEMSEKLRELAAVRQLPELLKKMK"
     gene            complement(2688..4439)
                     /locus_tag="PLE23_04120"
     CDS             complement(2688..4439)
                     /locus_tag="PLE23_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82483.1"
                     /translation="MKILSFIKILHTSLLFGVFLMVYQPIRAQIPAPDLYCIRNDTLQ
                     WSVPTVNCGNILGYEIYYSDAKSGPYSLLTTINNVSVNSYFHITAGKDFYYYMLTKAD
                     CPGQLPLSSDTLDNTDPPLVPIEVVNVLNGKCVITWKIPATTKQLSYIIYRTTPNGTL
                     PIDTVSNTNTYTDLTSNPLVQSESYFILALDLCGNASIFDKPHYSILADTSFDYCKRE
                     LRISWTKYQNWANGVEAYQIWASTNGANAIMVGTTDAATTDFTLKNIAKGDKLCIYIK
                     ALQAGSNLTTQSNEICIDINAIRPVEFIQPISASVLSNNSVELKWAWSADADLQSASV
                     LSGNSITNITSNTGYVYNAPAVQVNEFTVTGINPVHAPVFFKVSATDFCGAIYASFPV
                     STIHMTGKANEDKSNTLVWDFLAPAGIIVKNYTVFKNIAGNETVEGVLPANVNTLKDE
                     VDIDKQEEAEICYYVVANVEITLEDGTVLEEKIRSNDICLQQFASLFFPNAIAPDGLN
                     NEFRPLTVFAQNATYTLQVYDRFGQKVFETNDINQPWKGKKDGVALPLGVYVYYARLL
                     QPNGKTEERKGSVLLLR"
     gene            complement(4417..5178)
                     /locus_tag="PLE23_04125"
     CDS             complement(4417..5178)
                     /locus_tag="PLE23_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82484.1"
                     /translation="MNTCRFCHFTITPTKLFAALIIIFMMACTNGKINLKPYYYPLDE
                     LLKEGKVYEYKSDNLNMASSFWYYKTILRNDTTYLVGQNISQLMEVEQKTVERKTGNG
                     MVLHSNQIFLKDSTGKTNMYQVEVLEKSLFPFAVTDTNGIFLYKVKWHTDSLRHTTII
                     RNRRFIGFTEMTFDAKPIKCAKFELKELIEDFKDGYIEYPFHGVEYYGENIGLIKYVK
                     IIREGMQLSFTLNKIYTVTEFEKKYRTKLENPVIH"
     gene            5322..8648
                     /gene="secA"
                     /locus_tag="PLE23_04130"
     CDS             5322..8648
                     /gene="secA"
                     /locus_tag="PLE23_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMX82485.1"
                     /translation="MLGKLTNALSKLFGTKYDRDIKTYLPVVSNIHEEGAQLAGLSHN
                     ELRLVSLELKERIKDYLADIDAEITKINEQANIEEDIHQKEALYEQVDALKKDRDLQL
                     EVVLKDILPRAFALVKETAKRFTENTEIEVEATDHDRNLAAKKPYIKISADKAIYANS
                     WKAAGGDITWNMVHYDVQLIGGMVLHDGKIAEMMTGEGKTLVATLPAYLNGLSGQGVH
                     VVTVNDYLARRDSEWVGPIFEFLFLTIDCIDKYKPHTPERRNAYLCDITYGTNNEFGF
                     DYLRDNMVRSPEERVQRKHHYGMVDEVDSVLIDDARTPLIISGPVQHHTDEEEYVELK
                     PKVERVIETQKKLVTELLSQARQLFHDGVLGNEEGQAGMALLRAHRAFPKYRPLIKFL
                     SEEGVKVMLQKTENYYMQENNKNMHLVDAGLYFTIDEKNRNVELTDKGAEFMAKGNDD
                     PNFFIMPDLATSLMEIESNTSLTPEEIVEQKESLIRDYSVKSQRLHCTNQLLKAYALF
                     EKDEEYVVIDGQVKIVDESTGRLMEGRRYSDGLHQAIEAKENVKVGDITQTYATITLQ
                     NYFRMFHKLAGMTGTAETEAGEFWTIYKLDVVVIPTNRAVIRKDNEDLVFKTAKEKFN
                     AVIDEIENLTQQQRPVLVGTTSVDISEKLSRMLQMRGIKHNVLNAKQHQREAEVVAEA
                     GHPGAVTIATNMAGRGTDIKIGEIVKNAGGLAIIGTERHDSRRVDRQLRGRSGRQGDP
                     GSSTFYVSLEDNLMRLFHSERIAGLMDKMGHKEGEVIQHSMVTKSIERAQKKVEENNF
                     GIRKRLLEYDDVMNIQRGAIYKKRQHALDGDRLAVDINSILISLSDQIAQNAKSTGSY
                     QVFVEESIKLLGIDPDITEDTFNRTNRKELVEMYQDEVFDYYKRKFDKITEVLLPIIQ
                     NVYHNEGHRYKRIAIPYTDGSAHPLPISADLETTVKSNGKTIRTEIEKAITLAIIDEE
                     WMEHLRNMDELKDSVQSASFEQKDPLVIYKMEAFNLFQSLIGKINERISSYLLKGTVV
                     FQDGTRIEEARQKKTDLSKVSTNKTEEAMRQAATAASSAGQSAPKVETFVRTEKKIGR
                     NDPCPCGSGKKYKQCHGRDV"
     gene            8692..9348
                     /gene="deoC"
                     /locus_tag="PLE23_04135"
     CDS             8692..9348
                     /gene="deoC"
                     /locus_tag="PLE23_04135"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HMX82486.1"
                     /translation="MNIEGYIDHTLLKFNATDDAFYQLANEAILFGFASVCVPPSAVR
                     NLRRQFPELRICSVVGFPCGYQTTKTKIAEVTELSEEGVNELDFVINLQQFHSGNYEF
                     VENELYSLIGKCRENHILSKIIVESGSLSEIQLSTVCAIINKCLPDYIKTSTGMLLNG
                     YAVTPEEISRIRLLTESEIKIKASGGIRTRLQALELIQAGADRIGTSSSINIIQTMEN
                     "
     gene            9399..9755
                     /locus_tag="PLE23_04140"
     CDS             9399..9755
                     /locus_tag="PLE23_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMX82487.1"
                     /translation="MGTKAQVVINEDADVARIMQNFTNSNKSPDKTVHGWRIQISASV
                     DRKQVESMKKQIERDYSQYQPIISYDKPYYKLKIGAFLHKNKAESALNGVKTKYKSAY
                     LTRDVLKTAELGKDYE"
     gene            9768..11177
                     /gene="rodA"
                     /locus_tag="PLE23_04145"
     CDS             9768..11177
                     /gene="rodA"
                     /locus_tag="PLE23_04145"
                     /inference="COORDINATES: protein motif:HMM:NF037961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMX82488.1"
                     /translation="MSAPKSLIKENIDWVSFSLWLSLIAIGWVMIYAVDKHKFIEAGL
                     INFRSNTAKELLFISVSFVISLFVFFLDWKIWRVFAYPIFGLALFLLLSVLIFGVTIK
                     GQKSWFAISGFTFQPAELAKLGTCLALSSYLSLPGVSLKRTKTFLVSIGIILTPIFFI
                     ALQPDAGSSLIFLSFLIMLYRNGMSPLFAIFIFSIGTLFVLSLKVEIHYLVAALLLIG
                     SGILIYSFRFNKLYLSIGILALLVLTGFSLKYEFHNWVFSGIGLLILILSFIHYQNRK
                     QQLAFFTFMACLIAVSFSLSSYYTFNNILKPHQQDRLNVWLNPDKSDPRGALYNVLQS
                     KMAIGSGGITGKGFSNGILTKHSYIPEQKTDFIFCTVGEEHGFVGSFIVTGLFVWLLI
                     RLIMLAERQRSAFSRNFIYGITGILFFHFFINIGMTIGLVPVIGVPLPFVSYGGSSLM
                     SFSMMMAIVLKLDSHRNSL"
     gene            11187..12317
                     /gene="tgt"
                     /locus_tag="PLE23_04150"
     CDS             11187..12317
                     /gene="tgt"
                     /locus_tag="PLE23_04150"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMX82489.1"
                     /translation="MKFKKIHTDKHSKARVGIITTDHGDIQTPIFMPVGTAGTVKAVH
                     THELTEEIGAQIILGNTYHLYLRPGLQVLESAGGLHRFNKWDKPILTDSGGYQVYSLA
                     ANRKITEEGVKFKSHIDGSTHFFSPEYAIDIQRIIGGDIIMAFDECPPYPSDHNYVKK
                     SMELTHRWLKRCMSHFDNTPSKYSYEQSLVPIVQGGVYPDLRRQSAEFVCSVESEAYA
                     IGGLSVGESHELMYEMTDIVCNILPQEQARYLMGVGTPENIIESIGLGIDMFDCVLPT
                     RNARHGILYTHEGIINIKNQKWETDHSPIDEQSECRTSRQHSKAYLRHLMKQGEILGA
                     QLATLQNLSFFLSIVKQAREKILADEFVSWKNHILPIITRRL"
     gene            12322..>13053
                     /locus_tag="PLE23_04155"
     CDS             12322..>13053
                     /locus_tag="PLE23_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMX82490.1"
                     /translation="MKEFFSKIKILDLYIFKKYIFTFFFTVLLFTMISVVIDFSEKVE
                     DFLHEACTAHQILFDYYLNFIPYINWLLWPLISLIAVIFFSSRMAHNSEIISVFNAGI
                     SFRRMQISFLVPAILITMIHLFGNHYMIPLANKERLDFEHTYIHKNNDKGKTENVHLF
                     VSKDTKVYLKFFNKNDKTARDFRVEKFKDSDLVYILKAKSAEWDPKTLKWKLSNIEER
                     SFNGLKETIHVDKSGTRELKLNLSPD"
BASE COUNT         4060 a         2698 c         2644 g         3651 t
ORIGIN      
        1 catcaataca atcactgtta aagtataagc tgatattttc gtaactttct gccttatcca
       61 gcaagccgat attaagacca ccacgtgata ttgaattaat gtactctcca ggtcgtcctg
      121 aataaggctg aaggctggtt tgcccatcag gcgaatgtat cattcgtccg gtcatcggaa
      181 tcgaagcatt aagcataaac tcatccatac ctataagttt gagcgctttg atgcctctgt
      241 ctgatagtgc catattaatc gacctaccgg ctgacatctg tacgtggcgc atatctgctc
      301 ttttctcatg aagctgaact ttgaaaccgc gttgggcgag ccttatggcc agcagtgttc
      361 cactaagccc tgcccctata attacaatac tttttcctga cattttacat gtataatttg
      421 ctgcaattta attcaagaaa ttgaaatttc agccattact atagctcatt aatcttcaac
      481 ggtagaaaaa aatccctaaa atttcaacaa atttgatgga tatgtcaccg aaaacttgga
      541 atatatggct aaagcctttt aactttgaca aaaaaataga tatgagccaa actactcaat
      601 acaaatcgtc aataggctgg gtcggttctt tggtatttct gcttattagt ctgtggttac
      661 ttgttgatgc aacccactct aagtctgatt tccgaacctt ttttgccgga tttttggtat
      721 tatttttctg tcttactttg ttttatactt tgtctgacaa agactgatat tgccatccga
      781 acagccggaa taaaaaactg cccatactat taagtgcggg cagttttttt attttttgtt
      841 atagacaaat taatcgtatt tgaagaaaaa tcctcttctg ccgcaagctg cctttgcaaa
      901 atagccaaga ccgaagttga actcgagaca tacctggata gaataaggat tggagctttt
      961 atagtttgaa tattgaggta ccaatgtagg caatggaagt tttctgttct tcccttgctc
     1021 gcccaccaag tcctgaaatg cgtattcgaa cctggcccca agcatcatcg aaaaattggc
     1081 agcaccaaac acgtaagtac caaaaccaag agctgccgac caatagtcaa cataatcttt
     1141 tcctttctca agtgtaagtt catctaccaa gctgcctgta gtaatggttt gctttagggc
     1201 tgacaatctt gatagttttg gacctaactc gaggaatgtt ttgtcgccgt atagccggta
     1261 aagcaaataa aggtcagttg ccttccagtt gatcagataa tcttgccgga taccaagcgg
     1321 gccttctaca ccaaaagttt gtttgttgtc ggcaagcaat gcttctacag aaataccatt
     1381 gtggtaacca aagttgaccc caaaccttcc accataagca tatccggtag gcaaatcgta
     1441 acttactctg ccatcatcca tgatgttcga gttaagaaac cccttgaagc cgtatgtgcc
     1501 ttttgctgct gcttcaaacc atacctgtga ttgtgcagtg tacgacacac aaatgaataa
     1561 tagaacccaa agttttttca tactgatttt atattttgtt acaagacttt tccaacagca
     1621 ttttcgttgc cttggtttag tgaaatttga aaaatatctt tacttgcaaa tatgcctgaa
     1681 aatacatctc cttcctgcag caaaactaag caataattat ggtaattttg acaaattttt
     1741 caataatatg aaattcaaaa ctccgatacc cgtctcagaa ataagcagga tgatagaagc
     1801 cgaaatgtta ggtaatactc aacaaatggc taccggcatc aatgagatac acaaggtaac
     1861 accgggtgac atcgcttttg ttgatgttgt aaaatatttt aaaaaaacca tcaactctgc
     1921 agctactatc atcatactga atgaaaaaac cgaatgccct cccggaaaaa cactcctgct
     1981 tgttgacgag ccattcacag cttacaatta tctggtgcgt cagttcatgc cattcgaagc
     2041 tatggtgaat agtgtgagcc atcttgcaga catccatccg gatgccataa tagaaccggg
     2101 tgctatcatt ggccaacatg taagtatagg cgtaggtaca attattcagg ccaatgccgt
     2161 catctgcagt cacagcatag tcgggaagaa ttgtatcatc cagccgggtg ctgttatagg
     2221 gtctgatgct ttttatttca agaagaacaa gcagatatat cataaatgga actcatgcgg
     2281 gcgtgtcatt atcgaagatg atgtgtatat aggggccaac tgcaccatca accgcggtgt
     2341 gtcaggtgac accatcatcg gcgctggtag caagatagac tgcctggtac atattggcca
     2401 cggagtggtg atcggcaaaa actgtcttat agcagctcag accggcataa gcggaaaaac
     2461 tacactcgaa gataatgtcg tgctgtatgg ccaggtaggt attgcacaaa atatcaacat
     2521 cggggcaggt gcagtcattt cagctaaatc gggtatttct aaagatcttg atggcggaaa
     2581 aacatatttc ggtgtaccgg catctgaaat gtcagaaaaa ctccgcgagc tggcagctgt
     2641 aaggcaattg cctgagctac tgaagaaaat gaaataatca gctattttta tcttaacaac
     2701 aacacacttc cttttcgctc ttcggttttg ccattgggtt gcaggagcct tgcataatac
     2761 acatacacgc ccaaaggcag tgccacccca tcctttttgc ctttccatgg ctggttaata
     2821 tcgttggttt cgaacacttt ctgcccaaac ctgtcatata cttgaagcgt gtatgttgca
     2881 ttctgtgcaa aaacagtgag tggcctgaat tcattgttaa gcccgtcagg tgcgatggcg
     2941 ttggggaaaa acaaacttgc aaattgttgc agacagatat catttgacct tatcttttct
     3001 tccaaaacgg tcccgtcttc aagtgttatt tcgacatttg ctactacgta ataacagatc
     3061 tccgcttctt cttgtttgtc aatatctacc tcatccttaa gagtattcac attcgccggc
     3121 aatacacctt ctactgtttc atttccggca atatttttaa aaacggtata gttcttcaca
     3181 atgattcctg ctggtgcaag aaaatcccaa acaagcgtgt tggacttgtc ttcattggcc
     3241 ttaccggtca tgtgaatggt gctcacagga aacgaagcat atattgctcc acaaaaatca
     3301 gtggcagata ctttgaaaaa cacaggagcg tgtaccggat ttattccggt aacagtaaat
     3361 tcgtttacct gtacagccgg agcattatat acatacccgg tattggatgt aatgtttgta
     3421 atactgttgc ctgacagtac tgaagctgat tgaagatcgg catcagctga ccatgcccat
     3481 tttaattcaa cagaattatt gctcagtaca ctcgctgata ttggctgtat aaattccaca
     3541 ggtcggattg cattaatatc aatacaaatt tcatttgatt gagtggtaag atttgagccg
     3601 gcttgcagtg cctttatgta aatacacaat ttgtcgcctt tggcaatatt tttaagcgta
     3661 aaatctgtag tggcggcatc ggtcgtaccg accattattg catttgcacc attagtactt
     3721 gcccatatct gatatgcctc tactccattt gcccagttct ggtattttgt ccagcttatt
     3781 ctgagctcac gtttacaata atcaaatgat gtgtcggcca aaatactata atgcggtttg
     3841 tcaaatatac ttgcattgcc acacaagtcg agtgccaaaa tgaagtacga ctccgactga
     3901 accaaaggat tggaagtcaa gtctgtgtaa gtattcgtat tgctaacggt atctatcggc
     3961 aaggtgccgt tgggcgttgt cctgtaaata atatatgata actgcttggt agtagcgggt
     4021 attttccatg taatgacaca tttcccattg agtacattca ctacttctat cggcaccaaa
     4081 ggcggatcgg tattatcaag cgtatctgat gacaaaggca gctgaccagg gcaatctgct
     4141 ttggtgagca tgtaataata aaaatccttc cctgcggtta tgtgaaaata agaattgacc
     4201 gaaacattat tgatggtagt aagcaatgag tacgggcccg attttgcatc agaatagtaa
     4261 atctcgtaac caagtatatt cccacagttg acagtaggta ccgaccattg taaggtatca
     4321 tttctgatgc agtacaagtc cggggcaggt atctgtgcac ggatcggttg atataccatt
     4381 aagaaaactc caaacagtaa agatgtatgt aatattttaa tgaatgacag gattttcaag
     4441 ttttgtacgg tatttttttt caaattctgt tacagtataa attttgttaa gtgtaaagga
     4501 tagttgcatg ccctctctaa ttatttttac atatttaatc aggccgatgt tttctccata
     4561 atattcaacg ccatggaatg gatattcgat atatccgtcc ttaaaatctt caattaattc
     4621 tttcagttcg aattttgcac atttaatggg ttttgcatca aaagtcattt ccgtaaatcc
     4681 gataaagcgc cggtttctga taatagtagt atgtcgaaga gaatcggtat gccatttaac
     4741 tttatataaa aatataccat tcgtatccgt aactgcaaac gggaataaac tcttttccaa
     4801 gacttctact tgatacatat ttgttttacc cgttgaatct tttaaaaata tctggttgga
     4861 atgcaagacc attccattgc ctgttttacg ttctacggtc ttttgttcta cttccatcag
     4921 ttggcttata ttttgtccaa cgaggtaagt tgtatcattc cgcaatattg tcttgtagta
     4981 ccaaaaagaa gatgccatat tcagattgtc agacttgtat tcatatacct ttccttcctt
     5041 taacaattcg tctaatggat aataataagg cttgagatta attttaccgt ttgtacatgc
     5101 catcataaaa ataatgatca gggcggcaaa aagtttggta ggagttattg taaaatgaca
     5161 aaatctacag gtattcatga caatctgaca ttaaaatatg cgttcaataa aaatggaatg
     5221 aattctgcaa tcaaagttac gaatcattac acgataactt atcgtatatt tgcaccctaa
     5281 aataaagttt gcaccaagca gacacattta aatcaattaa tatgttagga aaattaacga
     5341 atgccctgtc aaaattgttc ggaactaaat acgaccggga tataaaaaca taccttcctg
     5401 tagtttcaaa catccatgaa gaaggcgcgc aattggcagg tttgtcacac aacgagttgc
     5461 gtttagtatc acttgagctg aaagaacgaa tcaaggacta cctggcagat atcgacgctg
     5521 aaatcacaaa aataaacgaa caggcaaaca tcgaggaaga cattcaccaa aaggaagctt
     5581 tgtacgaaca ggtagatgca cttaaaaaag acagagactt gcagctcgaa gtagtactca
     5641 aagacatttt acctagagca tttgcgctgg taaaggaaac tgcaaaacgt tttaccgaaa
     5701 acacagaaat agaagtagag gctacggacc acgacagaaa tctggctgcc aaaaaacctt
     5761 acatcaagat atctgctgac aaggccatct acgccaacag ctggaaagca gccggaggtg
     5821 atattacctg gaacatggtg cactatgacg tacagcttat aggtggaatg gtattgcacg
     5881 acggtaagat agccgaaatg atgaccggag aaggtaaaac actcgtagca acattacctg
     5941 cttacctcaa tggtctatca ggtcaaggcg tacacgtcgt gactgtcaac gattatcttg
     6001 cacgtagaga cagcgaatgg gtagggccga ttttcgaatt cctgttcctg accattgact
     6061 gtatagataa atacaaacca catacacccg aacgacgtaa tgcctacctg tgtgacatta
     6121 cctatggtac caacaacgaa tttggctttg attacctgcg tgataatatg gtgcgtagcc
     6181 ctgaagaacg cgtacaacgc aaacatcatt acggtatggt ggatgaggtt gactcagtac
     6241 tcatagacga tgcacgtact ccacttatta tatcaggtcc tgtacagcat cacaccgatg
     6301 aagaagaata tgtagaactg aagcccaaag tagaaagagt cattgaaacc cagaaaaaac
     6361 tagtaactga actactttca caagctcggc agttgtttca tgatggtgta ttgggtaatg
     6421 aagaaggtca ggccggcatg gccttgctca gagcacaccg ggcgttccca aaatacaggc
     6481 cacttatcaa attccttagc gaagaaggtg taaaggtcat gcttcagaag accgaaaact
     6541 actatatgca ggaaaacaac aaaaacatgc acctggtaga tgccggcttg tatttcacta
     6601 ttgacgaaaa aaaccgaaat gttgaactta cagacaaagg tgcagaattt atggccaagg
     6661 gcaatgatga tccaaacttt ttcatcatgc ctgacctggc tacaagccta atggaaatag
     6721 aaagtaacac ttcactgaca cctgaggaaa ttgttgaaca aaaagaatca ctaataaggg
     6781 actacagtgt caagtcgcaa aggctacatt gtacaaacca gctattgaag gcctatgcac
     6841 tcttcgaaaa agatgaagaa tatgtggtca tcgacggtca ggtcaagatc gtagatgagt
     6901 caacaggtcg tctgatggaa ggccgccgat actccgacgg actacaccag gccatagaag
     6961 ctaaagaaaa tgtaaaggtt ggtgatatca cccaaaccta tgctaccatt actctgcaga
     7021 attatttcag gatgttccac aagttggcag gtatgaccgg tacagccgaa accgaagccg
     7081 gtgaattttg gactatctat aagctcgatg tagtagtcat ccctactaac cgggctgtca
     7141 tccgcaaaga caatgaagac ctggtattta agactgccaa agaaaagttc aatgcagtaa
     7201 ttgacgaaat cgaaaacctg acacaacaac aaagaccggt actcgtaggt accacctctg
     7261 tagatatctc agaaaaactg agccgaatgc tgcagatgcg gggaataaaa cacaatgtac
     7321 tcaatgccaa acaacaccag cgcgaagccg aggtagtagc agaagccggg catccggggg
     7381 cggtaaccat tgccaccaat atggcaggta gaggtacgga cataaaaata ggcgaaatcg
     7441 taaaaaatgc gggcggtctt gctatcatcg gcacagaaag acacgactcg cgccgagttg
     7501 acaggcagct cagaggtcgt tcaggacgtc agggcgaccc tggtagctct actttctacg
     7561 tttcacttga agataacctc atgcgtttgt tccactcaga acgtatagcc ggactgatgg
     7621 ataaaatggg acataaagaa ggagaagtca tccagcattc gatggtcacc aaatctatag
     7681 aacgtgccca gaaaaaagtc gaagaaaaca acttcggtat caggaaaaga ttgctcgagt
     7741 acgatgacgt gatgaacatt cagcgtggtg caatatacaa aaaacgccaa catgcactgg
     7801 atggcgaccg cctggcagta gatatcaaca gtatccttat ttcattgtct gaccagatag
     7861 ctcaaaacgc caaatcaaca ggtagctatc aagtatttgt agaagagtca atcaaactac
     7921 ttggcattga cccggatatt acagaagata catttaaccg gactaaccgc aaagaactgg
     7981 tagaaatgta tcaggatgaa gtctttgatt attacaaacg aaagtttgac aaaataacag
     8041 aagtgctcct tcctatcata caaaatgttt accacaatga aggtcaccgc tacaaacgaa
     8101 tagccatccc ttataccgat ggcagtgcac atccactccc aatatcagcc gatctggaga
     8161 cgactgtcaa atcgaatggc aaaactatcc ggactgaaat agaaaaagcc ataacgcttg
     8221 ctattattga cgaagaatgg atggaacacc ttcgcaacat ggacgaactc aaagattctg
     8281 tacagagtgc ttccttcgag caaaaagatc ctcttgttat ttataaaatg gaggccttta
     8341 accttttcca gagtctgata ggaaaaatca atgaaaggat ttcttcttac cttctcaaag
     8401 gtacggtcgt ctttcaggat ggcacacgta tagaagaggc aagacaaaag aaaaccgacc
     8461 tgagtaaagt atcaaccaat aagactgagg aagcaatgcg ccaagcggct acagcagcct
     8521 cttctgccgg acagtcggca cctaaggttg aaacattcgt cagaacagaa aagaaaatcg
     8581 gacggaatga tccatgccct tgtggaagtg gtaagaagta caagcaatgt catggaagag
     8641 acgtatgatg atcttccact tgccaaatga aactaagaaa accgccacta aatgaatatc
     8701 gaaggatata tagatcatac attactgaag tttaatgcaa ctgatgatgc gttttaccaa
     8761 cttgcgaacg aggccatctt gtttgggttc gcttcggtat gtgtacctcc ttcggctgtc
     8821 aggaatttga ggagacaatt tcccgaactg cgtatttgtt cggtagtggg tttcccgtgt
     8881 ggctaccaga ctaccaaaac gaaaattgcg gaagtgaccg aattatcaga ggaaggtgta
     8941 aacgaacttg acttcgttat caatctccag cagtttcatt ccggaaatta tgaatttgtt
     9001 gaaaatgaat tatactcgtt gatcggaaaa tgccgtgaaa accatatctt gagtaaaata
     9061 atcgtagaat cgggttcgtt aagtgaaata caattaagta cagtatgtgc gatcattaat
     9121 aaatgcctgc ctgattacat aaaaacctct accggcatgc tcctaaacgg ttatgcagtc
     9181 acaccggaag aaataagccg aataagatta ttgactgaaa gtgaaataaa gatcaaagcc
     9241 tccggtggta ttcgcacccg gctacaagct ttggaactga tacaagctgg agcagataga
     9301 attggtactt cttcctccat taacattatt caaaccatgg aaaattaagc tttattatga
     9361 aaaaatatat tttactaatc tttgcaattt gcatttttgt gggaacaaag gcgcaggtag
     9421 tcattaacga agatgccgac gtagccagaa tcatgcaaaa ttttaccaac agcaacaaat
     9481 cacctgacaa aacagtacat ggctggagga ttcagatatc ggcaagtgtt gacagaaagc
     9541 aggtggaaag tatgaaaaag cagattgaac gtgattattc gcaatatcaa ccaatcattt
     9601 catacgacaa accttactac aagttaaaaa taggcgcatt ccttcataaa aataaagcag
     9661 aaagtgcttt gaatggagtg aaaacaaaat acaaaagtgc gtatctcaca cgcgatgtac
     9721 tgaaaacagc agaactgggt aaagattacg aataacaatg cccgccgatg tcagcaccta
     9781 aatcactcat taaagaaaac attgactggg tcagtttttc gttatggtta agccttatag
     9841 ccataggttg ggtgatgatt tatgcagttg acaaacataa atttatcgaa gccggattga
     9901 taaactttcg ctccaacacg gcaaaggagt tgttgtttat cagtgtatct tttgtaatca
     9961 gtttgttcgt ttttttctta gactggaaga tctggcgggt atttgcgtac ccaatatttg
    10021 gtcttgccct ttttctttta ttgtctgttc tcatcttcgg agttaccatc aaagggcaga
    10081 aatcttggtt tgcaatatcg ggctttactt ttcaacctgc tgagcttgcc aaactcggta
    10141 catgcctggc tttgagcagt tatctgagcc tgcccggtgt aagcctcaaa cgtactaaga
    10201 cattcctagt aagtattggt atcattctca ctccgatatt ttttattgca ttacaaccag
    10261 atgcgggctc ttcactaata tttttgtcat ttttgattat gctgtaccgc aatgggatga
    10321 gccctctgtt cgcgattttt attttttcca taggcacatt gtttgtcctg tcgcttaagg
    10381 tagaaatcca ctatctggtg gcggcactgc tgcttattgg ttccggaata cttatttata
    10441 gctttagatt taacaaactc tatttgagta ttggcatact ggcacttctt gtattgaccg
    10501 gattcagcct taagtacgaa tttcacaact gggttttctc aggcattggc ttgctgatac
    10561 tgatattatc attcatacat tatcaaaaca gaaaacagca gcttgcattc tttacattta
    10621 tggcatgcct tattgctgtg agctttagtc taagttcgta ttacacattc aacaacatcc
    10681 taaaaccaca ccagcaagat agattgaacg tgtggctcaa tcctgacaaa agtgatccgc
    10741 gaggtgcact ctataacgta ctgcagtcaa agatggcaat tggctcgggg ggtatcaccg
    10801 gcaaaggctt ctcaaacggc attcttacca aacacagcta tatacctgag caaaaaactg
    10861 actttatatt ttgtaccgta ggagaagaac acggttttgt gggcagtttt atcgtcaccg
    10921 gcctcttcgt ctggctactg ataaggttga ttatgcttgc cgaacgacag aggtctgcct
    10981 tttcacgaaa ttttatctat ggtattacag gtattctgtt tttccatttt tttataaata
    11041 ttggtatgac tatcgggctt gtaccggtca taggtgtgcc acttccgttt gtcagctatg
    11101 gcggctcttc attgatgagt ttttcaatga tgatggccat tgtactcaag ctggatagtc
    11161 accgaaattc attataaagc gaaaaagtga aatttaaaaa aatacatacc gacaaacaca
    11221 gcaaagcccg ggtaggtatc atcaccactg accacggtga tattcagacc ccaatattta
    11281 tgccggtagg tacagccggc actgttaagg ccgtacatac acatgagctc acagaagaaa
    11341 taggtgctca gatcatactg ggcaatacct atcatttgta tctccgccct gggctacagg
    11401 tattagagtc agccggcggt ctccacagat tcaataaatg ggacaaaccc atactcacag
    11461 atagtggtgg ataccaggta tattcgcttg ctgctaaccg aaaaataacc gaagaaggtg
    11521 tcaaattcaa atcgcacatt gatggctcaa ctcatttttt ttctccggaa tatgccatcg
    11581 atattcaacg tataataggt ggcgatatca ttatggcatt tgatgaatgt ccgccttacc
    11641 caagtgatca taattatgta aaaaaatcaa tggagcttac ccatagatgg cttaaaagat
    11701 gcatgtcgca ttttgacaat acgccttcaa aatacagcta tgaacagtcg ctggtaccca
    11761 tcgtgcaggg tggtgtttat cctgatttgc gcagacagtc ggcagagttc gtttgtagtg
    11821 tcgaaagtga agcctatgcc atcggtggat tgagcgtggg cgagtcgcac gaactgatgt
    11881 acgagatgac cgacatcgtt tgcaatatac ttcctcagga gcaagcccgc tacctcatgg
    11941 gcgtaggtac accggagaat atcatcgaat caataggcct cggtattgac atgtttgatt
    12001 gtgtactacc tacacgaaat gcacgacatg gcatacttta cacacatgaa ggcatcatca
    12061 atatcaaaaa tcagaaatgg gagaccgacc acagccccat agatgaacaa agtgaatgcc
    12121 gtaccagcag gcaacattcg aaggcatacc ttaggcatct gatgaagcag ggtgaaatac
    12181 tcggagccca attggcaact ctgcaaaacc tgtcattctt cctttctatc gtcaaacaag
    12241 cacgcgaaaa gatccttgca gatgaatttg tgtcttggaa aaatcatatc ttgcccatca
    12301 ttacaaggag attataacca aatgaaagaa ttcttctcga aaataaaaat actggatctc
    12361 tatatcttca aaaagtacat ctttacgttc ttctttaccg tattgctttt tacaatgata
    12421 tctgttgtca tcgatttcag tgaaaaggtt gaagacttcc ttcatgaggc ttgtacggcg
    12481 caccagattt tgttcgatta ttatttaaat ttcataccgt acatcaactg gcttttatgg
    12541 ccattgatat cactcatagc tgtgattttt ttttcatctc gaatggctca taattcagaa
    12601 attatttctg tatttaacgc cggtattagt ttcagacgga tgcagatttc atttttggtt
    12661 cctgccatcc ttattaccat gatacatttg tttggcaatc actacatgat acctcttgcc
    12721 aacaaagaaa gattagactt tgaacatact tacattcata aaaataatga taaaggcaaa
    12781 accgagaatg ttcatctgtt tgtaagtaaa gataccaagg tctatctcaa gttttttaat
    12841 aaaaatgata aaacggcacg cgatttcagg gtcgaaaaat tcaaagactc tgacctagta
    12901 tatatactca aagcaaagag cgcagaatgg gaccccaaga ccctaaaatg gaaactcagc
    12961 aatatagaag aacggagctt caacggccta aaggaaacca ttcatgtaga taagtcaggc
    13021 acgcgcgagc tgaagttaaa tctttcgcct gat
//
LOCUS       DAOQQY010000049        12907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1655_length_12907_cov_6.143557, whole genome shotgun sequence.
ACCESSION   DAOQQY010000049 DAOQQY010000000
VERSION     DAOQQY010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1655_length_12907_cov_6.143557"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PLE23_04160"
     CDS             complement(<1..1273)
                     /locus_tag="PLE23_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82491.1"
                     /translation="MMKYLTTGLNGIFLLLLLGVTTTGLTQNLNIPADHPANDVVERW
                     LISNSSKDIHPAIRPFNRNDWAQLILKKKINAHLNPKDSTNLLYSMIENQDFINCDSC
                     KPVLKSRKPLLTYFYKTPADLLEFKSDYFYLKLNPVFGMTIGKEKGNESNIFENSRGI
                     ELRGGIDSKVYFYSRILETQQRFENYITDRINRFNAVPGNGFYKNYNSSIFDVKNGFD
                     FNNATAYIGFNVTKHIGAQFGYGNNFIGNGIRSLLLSDYSNNYLYFKLNTKVGRFHYQ
                     NIFGELQAAGARDDIGDVLIPKKYFAAHYLSYAINKNWHAGIFETVVFARKDHFDFQY
                     LNPVILYRSVEAALGSPDNVLIGFNVSGNIAHTVQLYGQLMLDEFLFRELIKDNRGWW
                     ANKFGLQGGIKYINVLGVDHLDLQLEANLVMP"
     gene            complement(1306..2154)
                     /locus_tag="PLE23_04165"
     CDS             complement(1306..2154)
                     /locus_tag="PLE23_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HMX82492.1"
                     /translation="MKLFPVDRRTGLNRETFKNEYLKPSKPVVFTDFMDAWPAKEKWN
                     NDFFKEKYGDLVVPLFSEKSSNPGKKYMAPDTSMPFKQYLEMLESGPTQYRMFLFNIF
                     KHAPELIKDFSTPTIMDGWIKSFPFMFFGGEGSKVALHYDIDYSHVFLNQFHGRKRIV
                     LFSHDDSEKIYQYPFTVASYIDVNNPDYKKHPALRNVTGYECMLHPGETIFMPSGFWH
                     YIEYTDGGYSMALRSNDSVMTQVKGAFNIARHFIVDKSLNHLLGSKWMKMKDNMARQR
                     AEKYVR"
     gene            complement(2235..3425)
                     /locus_tag="PLE23_04170"
     CDS             complement(2235..3425)
                     /locus_tag="PLE23_04170"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HMX82493.1"
                     /translation="MASAKDKKSALSSLYSAALHQAPEYVAGLFAEQPEQRLYFHSYD
                     YCVHLSNLVMNGAAELNINPESAFKLRIVAWFYAVGRLFEYENFLPDSHRLMKEYLSA
                     YDIAPADMPSYDFFRLDDSGIDDHLAVGVLKDSLTVIRHGLADYHSPEILKKETEDTF
                     GITYSDQEWKNISYHDLSNKQLCTSWGVRHFSSRYNLALLNLRQQTAILAQDNKPAKP
                     KNNFNPTIYNFFRISFRNHIELSAMADNRSHIMISVNSIMMGIMISFITYQNLPATNP
                     SLLFPVVVFIITGMTSLIFAILAAKPRMWRNITKHSSEEEILKHLTSYSDFSNLSVEQ
                     YEQKLDQMFASRDLVKTNLKKELYYLGKILDTKNRYLVFSYNVFMFGFVVSMILFLSM
                     LIWS"
     gene            complement(3479..5572)
                     /locus_tag="PLE23_04175"
     CDS             complement(3479..5572)
                     /locus_tag="PLE23_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82494.1"
                     /translation="MIKKQLSIIIVILVCFTSFNIQAQESSKSKSASKLYERGEKLIL
                     VNNAARINTKGLETSPCFYNNGLLFVGEKKTGPIDRKTGLRYFDLYYSEIDKNGNALP
                     SDRFSKFISSPYNEGAACFNNDESILYFTSNNQQNGVAKSNGKSGVVLKIFQAQRGTD
                     DWINVQELPFCSDNFNTAHPSLSADGTKLYFASDRPGGQGGMDIYVSEKTGNSWGPPK
                     NLGPKVNTDGKELFPFVYGNDVLFFSSSGHKGLGGLDILFTDLTDAENFEPVLLDEPI
                     NSAKDDVGFIMQNNGQRGFFASNRTTGNGSDDIYSFEVKDGTLLNKVNMDTKLNITVV
                     DNANNQIIPGTAVYMVKAGKDGSLEDEEFYDVELVKENTNTTQIQLTRKKTMDLGEPA
                     GFTNDNGQISLTLEPNQNYVFFLKKQAYDLKEHFYATNSKPGSHNISIPIKLKDCATV
                     AGIVTNQKTGAPIPFADLKISKSCTGEVIPMTADGEGKFEICLPQTCSATFRAEKHGY
                     IQGLINVTNPKGNINQLTAEVKLLPFNNTPADKIEAEGTGTIAEGSVIVLENIYYDYN
                     KALIRPGAAKELDALYRLLKQFPAMRIELYSHTDSRGDNAYNQELSTQRAFSAKNYLV
                     ARGIEENRITAIGKGESQLRNKCADGVECTEEEHQYNRRTEVKVVSLGDPVEIRYGNK
                     GPEIIDAKKTQGTSR"
     gene            complement(5752..6708)
                     /locus_tag="PLE23_04180"
     CDS             complement(5752..6708)
                     /locus_tag="PLE23_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMX82495.1"
                     /translation="MKKIIYTILLTILCYGTSSAQQFPNYSLYIYNKMAYNPGYTGIP
                     DAPCFLALHRSQWIGIEGAPSTQIISANLPFLKKRLGIGLQLTNQSIGITRIFSTDMN
                     YAYHIPIGKGELSVALQGSLRYYAENYNDTRLHATQGVNIDNSIPVGLQNRYLPNFGT
                     GIYYSASNYYLGASAVRLIQNDIDFGNNDEFISSEKPHFFLMGGYSFILSDQVKLQPQ
                     TLVKYVNNAPVDIEVSVGLELLEKYMFALNYRAGGEIKAGSGDSADIVFGVQLSDRFM
                     FAVAYDITLSKLRKYNNGSVEAALQYCTGKFSNREVANPRFF"
     gene            complement(6722..>12907)
                     /locus_tag="PLE23_04185"
     CDS             complement(6722..>12907)
                     /locus_tag="PLE23_04185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82496.1"
                     /translation="ACNNAAIFDPVTFKITDGIPPLVQKPAADFTHQCNGSVGNALAF
                     SGWLSNRGGAKFTDQCLDSLSLTYSTIPANPQVPANSCNGSTEVTFRATDACGNIAET
                     KAKFILKDEVAPVINNVPKDTLFYCSMPALPPVPNNITATDNCTPNLFLDFSEKYESG
                     PCGPNTIIKRTWSTQDNCGNIRANTQIIQIIDTLAPVLTGIVPNDVTVSCENIPAAPP
                     VNLFTATDDCNASVPIEFKEINKAASCNAGYPIIRLWIAKDNCGNADTLSQKIYVIDN
                     LPPVLLGVPGDVTVTCSNIPTAPVIGSGITATDNCDTNVKILLDEVSTLGVCNNNKYQ
                     ITRTWTATDDCGNFVSKQQKISVTDNDFPVFTGVPENITVDCSMIPGIPQLGGSFKVT
                     DGCDPNVNVTYSQTTTQNPDSTSCDHYSYQLTRYWVANDDCGHVTTVQQKVTVQDKLA
                     PTLYCVDTFVIANNPLQCKAVSGINDLVFFSHGCSSISGTDSVTQTLFLTHSGADVMI
                     SPVDDLHFSLPVQGVPAAYLTGNILLRIDLNNVDAEEVSEFFRIYTEDGNLLGQTQHT
                     AGQCGNSSTFFTSITPDKINQWAFDGYVSFTLKTNGNGVNAINNFCQGGNVKLSLKFD
                     YITSPKISSKLFYSVDKAPFKQFVPGLLETFSLGNHEIKVKVEDCAGKFDSCNYILKV
                     KDVDAPTINCPADITAETLPLDCNLIYNLPPPVNVFDNCGFSSLSNTKTTPTNIFFQD
                     HPQAGKIPQDIIANFFGQPPIANGSLKIYLKADIANNGEFFFVYDENNNLLGQTGKGL
                     PALECNEYQEFSIPVTQTQISQWSLDGVIRFTLKANANTNSYTDFINPCGTLDAKGKD
                     PVSMVYFELIYPAYEIHYIVRDSNNVILTSQQYFSGSSKVELPVGKNKISYTVVDGGG
                     NIGSCSFNATILDKTPPKLTCKAGTIIDLNPSGLIPTIVSPYDLLVDTLFKIDNCGIE
                     TFTVTPNNFNCQMAGQGTTIKVIARDFSGNKDSCSSIVFFKNESLTPSISLDTCGGKL
                     TFIPDTTFKTPTPGTGNFFNYSWVGPNNFTSNLPSPVLQAPSAAYSGVYTLTVTGLTG
                     CQSSGSVMVNIDPSGLFKPTVYSNSPVCEGDSITIYTDWNNALNYTWTNVNTQQQFVT
                     AKNYLWLPADIKFAGAWSVKVGLNIGCNSLNSETHQVLVSLLSVTTPDTIQACKGIQV
                     QLSAQAIAGALFEWTAPNGFKYPGKNPNVPPIDGVYKVLVKNAQGCIGKDSLYVHVND
                     RPEITAISHSCPGCVSGTEACTIEPSVFPIDTGNYTYQWFNPSGGLFSIDSVASLANI
                     SANAGGQYQLIVTDKFSGCSSTPGKIDVNLNNTPATPLITQDDSTLPAKLCEGQPLII
                     KLTSNPYSGDVKYIWTTPLGKDTTTVPSLTYGSSTVNNAGMYQLQVLINGCLSNVSNE
                     IFAEVYPIPFPPPTFTNSPICDGDTLKLFADLIPGATYEWVGPTGVFSTLQNPLIADA
                     KPGDSGTYRVRITVNGCTSLYSAANNVFVGSIPAAPFVEQQCNGSVCASDPAAGCLLK
                     VIAPQAGSGSSFSLYNSAGVLLAGPQLSDSLYLKNLTQSGSGLKTFYVTVTTNGCESL
                     QSLPVQIQLDTIPKLKANAGQDIFICENIPLQICATAVNTGSGQWSQLSGPVVQILTP
                     FDACSNIAGSKGGQNVSFVWSLSNGACLHYSQDTVNVSISEKMQASAETPVQLCKDEP
                     VFINALAGNGVWTQPDAQASAGVVIQNPASASTAVFNLSANNSYIFDWVVDNGACGTE
                     IAQVTLGIYDDHAFAGDDRADCGLGCLKIPLQADIPVFGSGVWSSADSIVFFKDPFNP
                     ATEVCGLKNGIHKFVWTTNNGVCGKNSVDTIIINYQYLSLAVTDTLNVTFAESGFIDV
                     LQNDSLVSAYTLKMIQTPSKGKINNLGNGKFIYSPNPDFIGVDIMKYALCSNLCPDEC
                     TEAEVVFNVGGDIKCNAPTIITPNDDGVNDAFIVPCLAIENQYPANTVSVFNQWGDEI
                     FHASPYNNDWEGTYKDNPVPPGTYFYVIDLGDGTKPFAGFLIIKR"
BASE COUNT         3556 a         2583 c         2788 g         3980 t
ORIGIN      
        1 atggcatcac cagattggcc tccagttgta gatcgagatg gtccacaccg agaacattta
       61 tatactttat gccaccttgt agcccaaact tgttggccca ccagccccga ttgtctttga
      121 tgagctcacg aaacaagaat tcatcaagca taagctggcc gtatagctgc acggtgtgag
      181 caatattgcc tgatacgtta aagccgatga gtacattgtc cggactaccg agagctgcct
      241 caacagatct gtaaagtatc accgggttga ggtactggaa atcgaaatga tcttttctgg
      301 caaatacaac cgtttcgaat atgccggcat gccagttttt gttaatggca tagctcagat
      361 agtgtgcagc aaaatatttt ttcgggatga gtacgtcacc aatgtcgtct ctggcacctg
      421 cagcctgcaa ttctccaaaa atattttgat aatgaaatct gcctaccttc gtatttaatt
      481 tgaagtataa ataattatta ctgtaatcag aaagcaacaa ggaacgaatg ccattgccta
      541 taaaattgtt gccatagcca aactgcgcac ctatgtgttt tgtaacattg aagccgatat
      601 aagcagtcgc attattgaag tcaaaaccat tctttacatc aaagatactg ctgttgtaat
      661 tcttgtaaaa tccattgccg ggcactgcat tgaacctgtt gatcctgtct gtaatataat
      721 tttcaaatcg ctgctgcgtc tccaggattc tgctgtagaa atataccttt gagtctatgc
      781 cacctcgcaa ctctattcca cggctgtttt caaatatatt gctttcattt cctttctctt
      841 tacctatcgt cataccgaat acaggattta atttcagata aaaataatcg gatttgaatt
      901 ctaacagatc tgccggagtt ttgtaaaaat atgtaaggag tggttttcga cttttcaata
      961 ccggtttaca gctatcacag ttgatgaaat cctgattttc gatcatgctg tatagtaggt
     1021 tggtagaatc tttagggttg agatgtgcgt ttatcttttt tttcagaatg agttgtgccc
     1081 agtcattgcg gttaaatggt cgtatggcag ggtgaatgtc tttggaggaa ttactgataa
     1141 gccatcgctc caccacatcg tttgcgggat ggtcagccgg tatgttgaga ttttgtgtaa
     1201 gccccgtcgt tgttactcct aaaagcagaa gcaaaaatat accattgagc ccggtggtta
     1261 aatatttcat cattaacatt taatatccgg cggtaaagct acgaattacc ttacatactt
     1321 ttcagcacgt tgacgagcca tattgtcttt cattttcatc catttacttc caagcaggtg
     1381 attgaggctt ttgtcaacga taaaatgtct tgcaatgttg aatgctcctt ttacctgagt
     1441 cattacagaa tcattggaac gaagcgccat ggagtagcca ccatcagtat attctatata
     1501 atgccagaat cctgaaggca taaagatggt ttcgcccgga tgcagcatac attcgtagcc
     1561 ggtaacattt ctgagcgcag gatgtttttt gtaatccgga ttgttgacat caatataaga
     1621 tgcaaccgta aaaggatact gatatatttt ttctgaatca tcatgtgaga agagcacaat
     1681 gcgttttcgt ccgtgaaact gattgaggaa tacatgagaa tagtcaatat catagtgtaa
     1741 tgccactttt gagccttcgc caccaaaaaa catgaaaggg aaactcttga tccagccgtc
     1801 catgatggta ggtgttgaaa aatcttttat cagctcgggt gcgtgtttga aaatattgaa
     1861 aagaaacatt ctgtattgtg tcgggccgga ttcaagcatt tcaagatatt gcttaaaggg
     1921 catggaagta tcaggtgcca tgtatttttt acccggattg gaagattttt cggaaaacaa
     1981 tggcactact aagtcaccat atttttcctt aaaaaaatcg ttgttccatt tttctttagc
     2041 aggccaggca tccatgaaat cagtaaaaac aacaggtttg gatggtttga ggtactcgtt
     2101 tttgaaagtt tccctgttta agcctgttcg acggtcaact ggaaatagtt tcatcacaat
     2161 atgttttgaa attcagaaaa ttaaaactcc aaatatcggg ctaaaataaa atatcttttt
     2221 tttatcagga aattttaaga ccaaattaac atcgacaaaa ataaaatcat actaacaaca
     2281 aatccaaaca taaatacatt gtaagagaat accaggtaac ggtttttggt gtctaatatt
     2341 ttaccaaggt aatagagttc tttttttaga ttcgttttta ccagatcccg acttgcaaac
     2401 atttgatcga gtttttgttc gtattgttcg accgacagat ttgagaagtc ggagtaggaa
     2461 gtaagatgct ttagtatttc ttcttcactg ctgtgcttgg tgatatttcg ccacatccta
     2521 ggtttggcag ccaagatggc aaagatgagc gaagtcatgc cggtaatgat aaatactact
     2581 accgggaaca agagggaagg gttggtagcc ggcagatttt ggtatgtaat gaatgagatc
     2641 atgatgccca tcatgatcga attaacgctt atcatgatat ggcttcggtt gtcagccata
     2701 gcgcttaatt ctatatgatt gcggaagctt atcctgaaaa aattatagat ggtggggttg
     2761 aaattgtttt tgggttttgc cggtttattg tcttgagcaa gtatggctgt ttgttgcctt
     2821 aagttgagca aggcaaggtt atatcggcta ctgaaatgac ggacacccca agaggtacag
     2881 agttgcttgt tgctgaggtc gtgataactt atgtttttcc attcttggtc tgagtaggta
     2941 atgccgaaag tatcttctgt ttcttttttt aagatttcgg gagaatggta atctgcaagg
     3001 ccgtgccgta tgacagtaag cgaatctttg agtaccccta cggcgagatg gtcgtcaata
     3061 cctgaatcat caagcctaaa aaaatcgtaa gaaggcatgt ctgccggtgc tatgtcatag
     3121 gccgaaaggt attccttcat tagtctgtgg ctgtcaggca agaaattttc atattcgaac
     3181 agcctgccta ctgcataaaa ccaggcaacg atcctgagtt tgaatgcaga ttcagggttg
     3241 atgttgagtt ctgcggctcc attcattacc aaattggaca aatgaacaca ataatcgtaa
     3301 ctatggaagt acaatctttg ttcaggttgc tcggcaaata atccggctac atactccgga
     3361 gcctgatgaa gagcagcaga atacaatgaa ctgagagcag attttttatc tttagcagat
     3421 gccatattaa ctgtaaaact aatacatctt tagctgacag ccaataaaac aaggagtatt
     3481 atcgcgatgt accctgagtt tttttagcat caatgatttc cgggcctttg ttgccgtatc
     3541 gtatttctac aggatcacca agacttacaa cctttacttc tgtacgtctg ttgtactggt
     3601 gttcttcttc tgtacattcg acaccatctg cgcatttatt gcggagctga ctttcgcctt
     3661 tgcctatcgc tgttattcta ttttcttcga tacctcttgc tacgagataa tttttagctg
     3721 aaaatgcgcg ctgagttgac aattcttgat tataagcatt gtcgccacgg ctgtctgtat
     3781 gcgaataaag ttcgatcctc attgccggaa attgcttcaa caaacggtag agcgcatcga
     3841 gctctttagc tgcacccggt cttataagag ccttattgta gtcgtagtat atgttttcga
     3901 gtacaatgac gctcccttct gctatcgtgc ctgttccttc ggcctctatc ttatctgcag
     3961 gcgtgttgtt gaaaggaagt agctttactt cggcagtaag ctggttgata ttgcctttag
     4021 ggtttgtcac attaatgagg ccttgtatgt atccgtgttt ctcagcgcgg aatgtggcac
     4081 tacaagtctg tggcaagcag atttcgaatt ttccttcccc atcagcagtc attggtatga
     4141 cctcacctgt acatgatttg gagattttta agtcggcgaa tggaattggt gcccctgttt
     4201 tttggttggt gactatgcca gctacggtag cacaatcttt gagtttaatt ggaatgctta
     4261 tgttatggct ccccggtttg ctgtttgtgg cataaaaatg ctccttcaag tcataagctt
     4321 gtttttttag aaaaaacacg taattttgat taggctcgag tgtaaggctt atttgcccgt
     4381 tatcatttgt aaatcctgct ggctcgccga ggtccatggt tttctttctt gtgagttgta
     4441 tttgggtagt gttagtgttt tcctttacaa gctctacatc atagaattct tcatcttcga
     4501 gtgaaccgtc ttttccggct ttgaccatat atactgctgt gcccggtatt atttgattgt
     4561 tagcattgtc cactacggta atattgagtt tggtatccat gtttacctta ttgagcaatg
     4621 taccatcttt tacctcgaaa gaataaatat cgtcactgcc attgccggtt gtgcggttag
     4681 atgcgaagaa gcctctctgg ccattgtttt gcattataaa cccaacatca tccttggctg
     4741 agtttatcgg ttcgtcgagc aataccggtt caaaattttc tgcatctgta agatccgtga
     4801 acaagatatc caagccgcct aaacccttgt ggcctgacga agagaagaaa agcacatcat
     4861 tgccatatac aaatgggaat aactccttgc catcagtgtt tactttgggc ccgagatttt
     4921 tgggtggtcc ccagctatta cctgtttttt ctgatacata aatatccata ccaccctgac
     4981 caccaggcct gtctgatgcg aaatacaatt ttgtgccatc tgcagacagt gatgggtgtg
     5041 ccgtattgaa attgtcagaa cagaaaggca attcctgaac attaatccag tcatcggtac
     5101 cacgttgtgc ctggaatatt tttaatacta cacccgattt gccgtttgat ttagctacac
     5161 cattctgctg attgttgcta gtaaaataaa ggatgctttc atcattatta aaacaagccg
     5221 ccccttcatt ataagggctg ctgatgaact ttgaaaatct gtcagaaggt aaggcattgc
     5281 catttttatc tatttcagaa taatacaagt caaagtaacg caaacctgtt tttcggtcaa
     5341 tagggcccgt tttcttttct ccaacaaaca aaagcccatt attgtaaaaa caaggtgatg
     5401 tctcaagacc tttggtgtta atacgtgctg cattgttgac caatatcagt ttttcaccac
     5461 gttcgtaaag ttttgaagcc gatttggact tagatgactc ctgcgcttga atgttaaaag
     5521 atgtaaaaca aacaaggata acaataataa ttgaaagctg ttttttaatc atgtctttat
     5581 caatattata ctaagaacga aaatcaattc acaaatatta taaaatacta ccaaaccgta
     5641 gcaaatctat taaagattaa cggataaaaa tctcatttct caaataagtg tcaaggagaa
     5701 aatctttatg atacggcttc gtcttttgtt aaacttctct gtgcgttttt atcaaaaaaa
     5761 tcgagggttg gccacttctc tgtttgaaaa tttgcctgta cagtactgca gtgcagcttc
     5821 taccgaacca ttgttgtatt ttcttaattt ggagagtgta atatcgtatg ctacagcaaa
     5881 cataaatctg tctgataact gaacaccgaa tactatatca gccgaatcgc ctgaaccggc
     5941 ttttatttca ccacctgccc tgtagtttaa ggcaaacatg tatttttcaa gcaattcgag
     6001 cccgacagac acttcaatat caaccggggc attgtttaca tatttaacaa gcgtctgtgg
     6061 ctgtagcttt acctggtcac ttagtatgaa tgaatatccg cccatcagga aaaagtgggg
     6121 tttttcagaa gaaataaatt cgtcgttatt gccaaaatct atgtcgttct gtataagcct
     6181 tactgcagat gctcccaaat aataattgct tgcagagtaa tagatgcctg taccaaagtt
     6241 aggtaaatat ctattctgta gcccgaccgg aatactattg tcaatattga caccctgtgt
     6301 agcatgtagt cttgtgtcat tgtaattttc tgcatagtat ctcagacttc cctggagtgc
     6361 tacactcagc tcacctttgc caatgggtat gtggtaggca taattcatgt cggtagaaaa
     6421 aattctggta atacctatgc tctggttggt aagctgaagc ccgatgccga gtcttttttt
     6481 gagaaatggc agattggctg atattatctg tgtacttggg gcgccttcta tgcctatcca
     6541 ctgactacga tgcagcgcca ggaaacacgg agcgtcggga atgccagtat agcccggatt
     6601 gtatgccatt ttattataaa tgtataatga gtagttagga aactgctgtg ctgagctggt
     6661 tccataacat agtatagtga gcaatattgt gtaaattatt tttttcatgg tcaatactgg
     6721 tttacctttt tattattaaa aatccggcaa atggcttggt tccgtctcca agatctatca
     6781 cataaaaata tgtgccgggt ggtacaggat tatccttgta tgtaccttcc cagtcattat
     6841 tgtaaggtga tgcatgaaat atttcgtcac cccattggtt aaatacagat accgtattgg
     6901 ccggatactg gttctctata gccagacaag gtacaatgaa tgcatcattt actccgtcat
     6961 catttggtgt gatgatcgta ggtgcattac acttgatgtc accacctaca ttgaacacta
     7021 cttctgcctc tgtacattca tccggacaaa gattactgca caatgcatat ttcatgatat
     7081 caacaccgat aaagtccgga tttggtgaat agataaattt gccattgccc aggttattta
     7141 tcttgccttt ggatggagtt tgtatcattt ttaaagtgta ggcagacacc agcgaatcat
     7201 tttgcaatac atcgataaaa cctgattcag caaaagtaac attcaacgtg tctgtaactg
     7261 ccagtgacag atactgataa ttgatgataa tagtatctac tgaatttttg ccacaaacac
     7321 cattgttggt ggtccagaca aatttgtgga tgccgttttt gagtccgcac acctcggttg
     7381 ccgggttaaa cggatctttg aagaatacta tactgtcggc tgatgaccaa actccagagc
     7441 caaatacagg aatatctgcc tgcaatggta tttttaaaca tcccagaccg cagtctgccc
     7501 tgtcatcacc tgcaaaagca tggtcgtcat atatacccaa tgtgacttgt gcaatttccg
     7561 ttccgcaagc accgttgtcc actacccagt caaatatgta agaattattg gcacttaggt
     7621 tgaatactgc cgtagatgct gaagccggat tctgtatgac aacgccggcg ctggcttgtg
     7681 catctggttg ggtccacact ccattgcctg ctaaggcatt gatgaataca ggctcatctt
     7741 tacacaattg taccggtgtc tcagctgatg cctgcatttt ttccgaaatg cttacattta
     7801 cagtgtcctg gctgtaatgg aggcaagccc cattgctcaa agaccataca aagctgacgt
     7861 tttgcccacc cttactgcct gcaatgttgc tacaggcatc aaaaggtgta aggatttgta
     7921 caacaggtcc gcttaattgt gaccattgcc ctgatcctgt attgacagct gtagcacata
     7981 tctgtaatgg aatgttttcg cagataaaaa tatcctgacc tgcattggcc ttgagcttag
     8041 gaatggtgtc aagctgtatc tgcaccggta aggattgaag cgactcgcat ccattggtag
     8101 tcacagtaac gtagaaggtt tttagtcctg aaccactttg tgttaaattc ttgaggtata
     8161 acgaatcgct cagctgtggg ccggcaagca gaacacctgc agaattatac aagctgaagc
     8221 tactgccact tcctgcctgt ggtgcaataa ccttaagcaa acaaccggct gcaggatcag
     8281 aagcacatac acttccgttg cattgctgtt ctacaaatgg cgctgcagga atactaccta
     8341 caaaaacatt attagctgct gaatatagtg atgtacagcc attgacagtt attcttactc
     8401 ggtaggtacc tgagtctccc ggtttggcat cggcaataag gggattctgg agtgtggaaa
     8461 atacacctgt aggccctacc cactcataag tggcacccgg tatcaggtcg gcgaacaatt
     8521 ttagtgtgtc accatcacat atcggagagt tggtaaatgt tggtggtgga aaagggatag
     8581 ggtacacctc ggcaaatatt tcattagaca cattcgacaa acatccgttg atgagtacct
     8641 gtaattggta catgcctgca ttgttgacgg tagaagatcc gtaagttagc gacggcacag
     8701 tggtggtgtc tttgcccaat ggcgttgtcc agatgtattt tacgtcacca ctgtaaggat
     8761 tagacgtcaa tttgatgatc aaaggttggc cttcacagag cttggcaggc aaggtcgaat
     8821 catcttgagt aataagtggg gtggcaggtg tattattgag atttacgtca attttgcccg
     8881 gagttgaaga acagcctgaa aacttatcgg taacaataag ttgatattgg ccgccggcat
     8941 tggctgagat attggcgagt gatgctacac tgtctatgct aaacagccca cctgaaggat
     9001 tgaaccactg ataagtataa ttgcctgtat caataggaaa gacggatggt tctatagtac
     9061 aagcttctgt accacttaca catcccggac acgagtgact gatggcggtt atctcaggtc
     9121 ggtcatttac atgtacatac aagctgtctt ttccgataca gccttgagca tttttgacca
     9181 aaaccttgta aacaccgtct attggtggta cattcggatt cttgcccgga tatttgaacc
     9241 cattaggtgc agtccactca aacaaggcac cggcaatggc ctgtgcactc agttgtacct
     9301 gaatgccctt acacgcttgt atggtatcag gtgtagttac cgacaataat gacacaagta
     9361 cctgatgtgt ttcagaattg agcgaattgc aaccaatatt cagtcccact tttacactcc
     9421 atgctcctgc aaatttgata tctgcaggca gccagaggta attcttggca gttacaaatt
     9481 gttgctgtgt gttgacattc gtccaagtgt agtttagagc attgttccag tcggtataaa
     9541 tggtgattga gtcaccttca cataccggac tgtttgaata aaccgttggt ttgaataatc
     9601 cggacggatc aatatttacc ataacactac ctgatgactg acaacctgtg agaccggtga
     9661 cggtaagcgt atatacacca ctgtaagctg ctgaaggtgc ttgtaataca ggtgaaggta
     9721 gattggaagt aaaattattc ggccctaccc aggagtagtt gaagaaattt cctgtaccgg
     9781 gtgttggtgt cttgaaagtc gtgtccggga taaaggtcaa ttttccacca catgtatcaa
     9841 gcgaaatgct tggtgttagg gattcattct tgaagaatac aatagaacta cagctgtctt
     9901 tgtttccgga aaaatctcgg gcaatgacct ttatggtagt accttgtccg gccatttggc
     9961 agttgaagtt gtttggtgtg acggtaaagg tctcgatacc acaattgtct attttgaaca
    10021 atgtatccac gagcaagtca taaggactta caatggtagg tataagaccc gatgggttaa
    10081 ggtcgatgat ggtacctgcc ttgcaagtca gttttggagg tgttttatcc aaaatggtag
    10141 cgttgaagct gcaactgcct atgttgcctc caccatccac tacggtatag cttattttat
    10201 ttttacctac aggtaattct accttagagc ttccggaaaa atattgctgc gaagtaagta
    10261 tcacattgtt cgaatcacgg acgatataat ggatttcgta ggccggatag atgagctcga
    10321 aatataccat actcaccgga tctttgcctt tggcatcaag tgtaccacaa gggttgatga
    10381 agtcggtata gctattggtg ttggcattag cctttaaggt aaaacgaatg acaccatcca
    10441 aagaccactg cgaaatctga gtttgcgtca ccggaatgct gaattcctga tattcgttgc
    10501 actcaagtgc cggtagccct ttgcctgtct ggccgagcaa attattgttt tcatcataca
    10561 cgaagaaaaa ttcaccgttg ttggcaatat ctgccttcaa ataaatcttt aatgaaccat
    10621 tggctattgg tggctggccg aagaaattgg ctataatgtc ttgcggaatt tttcccgctt
    10681 gcggatgatc ctgaaagaaa atattggtag gtgtggtctt ggtgttggaa agtgaactaa
    10741 agccacagtt atcaaataca tttacaggtg gagggagatt gtaaatcaga ttgcaatcaa
    10801 gtggtagtgt ttcagcagtt atgtcagcag ggcaattgat tgtaggggca tccacatcct
    10861 ttactttcaa aatataattg cacgaatcaa atttacctgc acagtcctcc actttaacct
    10921 tgatttcgtg attgccaagg ctgaaagttt ccaggagtcc gggtacgaat tgcttgaaag
    10981 gtgctttgtc aaccgagtaa aaaagttttg aactaatttt tggagaagtg atataatcaa
    11041 attttaaaga aagcttaaca ttgccaccct gacagaaatt attgatggca ttaacaccat
    11101 ttccgttcgt tttaagtgta aatgatacat aaccgtcaaa ggcccattga ttgattttgt
    11161 cgggcgttat gctcgtaaaa aatgttgatg aattgccgca ttgtccggca gtgtgctgag
    11221 tctgtccgag caaattacca tcttctgtat atattctgaa aaattcagat acttcctcgg
    11281 catcaacatt attaaggtca atccttaaca gtatattgcc ggtaagataa gctgcaggta
    11341 cgccctgtac aggcaaactg aaatgaaggt catcaaccgg tgatatcatt acatcggcgc
    11401 ccgagtgtgt aagaaagagg gtttgggtaa cggagtcggt accactaata ctcgaacagc
    11461 catgcgagaa aaataccagg tcgttgatac cacttacagc tttacattgc aaaggattat
    11521 tagcaattac aaatgtatct acgcaatata aggtaggtgc cagcttgtcc tgtacagtaa
    11581 ctttttgttg tacagtagtg acatggccgc agtcatcgtt ggctacccag taccgcgtaa
    11641 gctgataact gtaatgatcg catgatgtag aatccggatt ttgggttgtc gtctggctgt
    11701 aagtgacatt gacatttggg tcgcaaccat ccgttacttt aaaactccct cccagctgag
    11761 gaatgcccgg aatcatactg cagtcaacgg taatattctc cggaacacca gtaaataccg
    11821 ggaaatcatt gtcagtaaca cttattttct gctgttttga tacaaaattt ccacaatcat
    11881 cggttgcagt ccaggttctg gttatctgat atttattgtt gttacatact ccgagggtgg
    11941 acacttcatc aagtaggatt ttcacattcg tgtcgcagtt gtcggttgct gtaataccac
    12001 tacctatgac aggggcagta ggaatgttgc tgcaggtaac cgttacgtcg ccgggtacgc
    12061 ctagcaatac gggtggcaga ttgtcaatta cgtatatttt ctgcgaaagg gtatcagcgt
    12121 taccacaatt atcttttgct atccaaagtc ttataatggg gtagcctgcg ttgcaactgg
    12181 cggctttatt aatttcttta aattcgattg gaacgcttgc attacagtcg tcagtagctg
    12241 tgaagaggtt gacaggcggt gctgcaggaa tgttttcaca actgaccgtg acatcattgg
    12301 gtacaatacc tgtaagtaca ggagccaaag tgtcaattat ctgaatgatt tgcgtatttg
    12361 ctcggatatt gccacaattg tcttgggtac tccatgttct ttttatgata gtattgggac
    12421 cgcatggacc tgattcgtat ttttcactaa aatcaaggaa tagatttggt gtacagttgt
    12481 cggtagcggt tatgttgttg ggtactggag gcaaagcagg cattgagcaa tagaacagtg
    12541 tgtctttcgg aacattattg attaccggag caacttcatc ttttagtata aattttgctt
    12601 ttgtctcagc gatgttacca caggcgtcag ttgctctgaa ggttacttcg gtacttccgt
    12661 tacacgagtt ggcgggtact tgaggattag ccggaatggt tgaataagta agcgaaagac
    12721 tgtcaaggca ctggtcggtg aattttgcac cacctctgtt gctcagccag cccgaaaatg
    12781 caagcgcatt gcctacagaa ccattgcatt gatgagtaaa gtctgcagct ggtttttgta
    12841 ctaaaggagg gataccatca gtaattttga aagtaaccgg atcgaatatt gcggcattgt
    12901 tgcatgc
//
LOCUS       DAOQQY010000050        12901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1658_length_12901_cov_5.791375, whole genome shotgun sequence.
ACCESSION   DAOQQY010000050 DAOQQY010000000
VERSION     DAOQQY010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12901
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1658_length_12901_cov_5.791375"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1156)
                     /locus_tag="PLE23_04190"
     CDS             complement(<1..1156)
                     /locus_tag="PLE23_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HMX82497.1"
                     /translation="MNGFSENVQDIIKQFKFRNEIETLDEADILFSLIEKFCSPKVEL
                     HPDKLPPLAMGYVYEDLVRRFNEENNEEAGEHFTPREIIDLMTHLVFLPVKDKIKQGT
                     YLIYDPCVGSGGMLTIAKNFLLNPDGLIKSNATIHLYGQESTPFIYATCKSDMLIKGE
                     DPDKIAYGSTLSAYGFDKDLKFDFMLTNPPYGKTWAPDKKALGVGPKGAIIDKRFMLK
                     GSYKEEAVTSRVNDGQLMFVLHMLSKMKDTELGSRIASVHNGSALFTGDAGQGESEIR
                     KYIIENDYLEAIIALPNDMFYNTGIPTYIFLITNRKSKHRKGKVQLINATTEDFYSKM
                     RKSLGKKRVEFLPEHIDKIYYSMKQAIEENFILDVLQNYTTYQSYYALLKK"
     gene            complement(1315..1485)
                     /locus_tag="PLE23_04195"
     CDS             complement(1315..1485)
                     /locus_tag="PLE23_04195"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HMX82498.1"
                     /translation="MSLNTQSLQPIINFLWTVADDVLVNTYQKGKYKDVILPMVVIRR
                     LDLLLEHTKEQV"
     gene            complement(1899..3611)
                     /locus_tag="PLE23_04200"
     CDS             complement(1899..3611)
                     /locus_tag="PLE23_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82499.1"
                     /translation="MIRLIFLKVCLLCFTVVVTAQDIPSFPGAQGFGAKATGGRGGKV
                     IYVTNLNANGPGSLNDALAQEGPRYILFKVSGLIDAAAELVYGNVTIAGQTSPGGIIT
                     RGFLVDEVYEPDGTGDNIILRHLRSRPHDPDLVPASHYILDDALRLDGASNIIIDHCS
                     FANARDEAVQISQSKDVTMQNCILSETVGDHYDRGGMLINYSTPEHPQDRLSIHHNTW
                     NRIAARLPEIICESPYCAAAKLKLELSNNLIWDPQNNIWYGPNIDPSAEENKFFLDLN
                     WVNNYMYVRPDFPFGMMEIRLIQFAQNSIFGSGNKMNIYPNYSDYQLCYCCNDFNAPG
                     ANPNTDFGAAQKLTARHNFPEVTYLQTESLPAYMADNVGAFPRDQMDNRLMSHVDQLS
                     IDPNPVNGTDYNFDGHIVNPNPPESPSDKDNDGMPDYWEAAHGLNPDVQDHNGTELSL
                     AITGVEGYTNLECYLNCLSDALISGQSVAACGITLSTPSPEKDAGILIFPNPSSGSFE
                     VQHGDFKHADIEVFNYLGTQVFKTNYSGRSMKIDTQLQAGLYTMVVRDNGKVADIRKI
                     LIIR"
     gene            complement(3738..3811)
                     /locus_tag="PLE23_04205"
     tRNA            complement(3738..3811)
                     /locus_tag="PLE23_04205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3775..3777),aa:Ala,seq:ggc)
     gene            complement(3956..5494)
                     /locus_tag="PLE23_04210"
     CDS             complement(3956..5494)
                     /locus_tag="PLE23_04210"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HMX82500.1"
                     /translation="MKNTLLGIFSIFCVQLFAQNQPPAILNVSSAQNAKTVTYNFTLE
                     DDENDVCTVELHVFKKSGSGFEPLTVNGITGDIGNNISTGTKAISVPLPSDGEYRINI
                     VAYDGHSFDLQAIVDRVDTLHLYSDLKWLEGTRHRTAGAAKLKAVQDIIESLFTSLHY
                     EPSIHTAKFGDYQLKNYIADHYGASSPGRILINDAHYDCVPFGPGADDNATGVAGMME
                     ISRIIADYHFRKSIRFIGFDVEEDGLIGSKAYVGQGGLSVKDTIEGVLNFEMIGYYSD
                     KPNSQTLPNGFNLLFPAQSAAIAADSYKGNFITNVRNSNSKGISDAFEKSATTYVPEL
                     KVVTLEVPGNGALTPDLLRSDHASFWTAGKPALMLTDGANFRNKNYHTANDRVDSLNM
                     WFMGNVVKASIGALLEEANIIHGDVYSSDLQVLTAVDQLPGISVEIFPNPMNEILHIT
                     SQNAGNCTVDIQTLSGELIFNKKYDSEASGKIEIGTSKWPAATYLVKFETEQGTILRK
                     IVKN"
     gene            complement(5585..6430)
                     /locus_tag="PLE23_04215"
     CDS             complement(5585..6430)
                     /locus_tag="PLE23_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMX82501.1"
                     /translation="MRYFTALFALLFTLPSIAQSSLLWKIEGKDIKKPSYLFGTIHLI
                     PASDYFEPKGLDAAFNSCELLVGEMDLSDITGMSLEVMANMQMKGDTTLDMLLTKEEY
                     DKLDKRFLSTLGIGLDFMKSFKPIFLSTMLMTPGEGEETKSYELELMEKAKKKGIKMA
                     GLETLQEQMGFLDVLPYSFQAKELVKMLDVKTDDSDFQKMVQVYKSQNLDELYAIIKG
                     QSEGSAMEDVLLVKRNQNWIQKIVNIAQTQPSFIAVGAGHLGGENGVIHLLKAQGFKV
                     TPVLD"
     gene            complement(6506..7783)
                     /locus_tag="PLE23_04220"
     CDS             complement(6506..7783)
                     /locus_tag="PLE23_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HMX82502.1"
                     /translation="MFDFKTPGQAVSIVKSDDRVFIQGGAATPNILIDALSERKDELQ
                     NVEICHIHTEGPAPYVLPQYDGAFRTNAFFVGGNIRPHISHSHVQYIPIFLSEVPLLF
                     RRGVMPLDVAMVHVSPPDLHGFCSLGVSVDVTKAATDVAKKIIAVVNPKMPRSLGDGL
                     IHASRFDAMVYSEADIHTSQLPSLSDNELAIGKHIANLVEDRATLQLGIGAIPNAVAR
                     FLVNHKDLGIHTEMFSDGILPLIEKGVITNKYKHKYPGKTVGSFAIGTQKLYDFIHDN
                     PAVVMLDAAYVNDSSIIRKNPKMTAINSAIEIDLTGQVCSDSIGTSIYSGVGGQMDFI
                     RGASLSEGGKPIIAIASVTKKGVSKIVPTLKTGAGVVTSRAHVHYVVTENGVAELYGK
                     NIAQRVKELIKIAHPDHQARLEKEGYDLYGKCW"
     gene            complement(7790..10186)
                     /locus_tag="PLE23_04225"
     CDS             complement(7790..10186)
                     /locus_tag="PLE23_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMX82503.1"
                     /translation="MKIYLKILSALVIIIFASASAKAQHNISALSIDQIMQGEKFTGY
                     SPENISWSIDGKNIYFDWNPEIKPVREKYAYNLKTGKTEKMSIAALRDMFSDGGEYDP
                     TGKWKVFSKNGDIYIYDVASGQMKQVTDTYVGEYQPVFSADGQYILYLSYGNVFSWNK
                     NTGALRQLTNFIKGEKRHDGKRNAQESYLEKQQFELFEVLRQRRDIAKMNKALDDSLK
                     IYRAKEFYYGEKNMTAIVADPTMKSVCFQLERPAVNKMPVVPDFVNPSGYTRDLDTRN
                     KVGSKQSSYELLIFDIKKDTVIAFDRSILPGIFDKPSYYRDYNKDSIYTDVFKTPREV
                     NYFGPVYNNTGQAIFEVKAHDHKDRWIVLIDLSKENPFTVLDRQHDDAWIGGPGIVEW
                     MDAPGRIGWINDQTVWYQSEVTGYSHIYTQNTETLEKKALTSGDFEILEARLSKDRKN
                     FFITANKPTPFEHHFYTLYVNGGEMKQITQLTGNHEVSVSPEEKYLAIRYSYTNKPWE
                     LYLMENKPGAKMQKITSSITASFNNYKWREPAIVMIPASDGVKVPARLYKPERPNGAG
                     VVFVHGAGYLQNVHKWWSTYYREYMFHNMLADNGYTVIDIDYRASEGYGRDWRTAIYR
                     YMGGRDLDDQVDGAKYLVDSLQLDKDRIGIYGGSYGGFMTLMAMFTKPGVFKCGAALR
                     SVTDWAHYNHEYTSNILNTPTEDSVAFRRSSPIYFAKNLRGRLLMLHGMVDTNVHYQD
                     VVRLAQRLIELKKDNWEMAVFPVEDHGFQEATSWTDEYKRIYKMFDEELTQQYKPIKN
                     "
     gene            complement(10383..12527)
                     /locus_tag="PLE23_04230"
     CDS             complement(10383..12527)
                     /locus_tag="PLE23_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMX82504.1"
                     /translation="MKRIVFAVLLLLLVKIYSYADEGMWLPIFLKQLNEKEMRAAGMK
                     LRAEDIYSINKGSLKDAVLHFGGGCTGEVVSPKGLLLTNHHCGYGQIQKHSTLEHNYI
                     RDGFWAKNMSEELPCEGLTVTFISRIEDVSTQVLLGVNDKMTESERQSVIDQNIAKVS
                     KSLVIQAYEAVSIKPFFTGNQYFAIISVTYKDVRMVGAPPESIGKYGSDTDNWVWPRH
                     TGDFSVFRIYAGKNNLPADYSPDNVPYVPKHYFPISLDGVKENDFTMIFGFPGRTDEY
                     LPGIGVQQATEVLNPVRISLREKALKIMDAKMRTNPATKIKYASKYASIANAWKKWIG
                     ESLGVKKTDGLGRKMKYEADFTKRINADPALNQKYGQLLPEFEKKYSEYKRYAVAREA
                     IAEVFSRILDFTRMGNQAIQLIKAFDSQGETEYSKQATKARDGLAGFYKNFEADIDKE
                     MFVELILIYKANVESGLQPATITKLSKEQFASMFDSSNLINEAKMNTILALPATEAVK
                     LLREDALVALLKEVYEFNQNSISPKANTYSAEINTLQRTYMQAQMEVFKEKKFYPDAN
                     STLRVSYGKVKPYSPKDGIKYDIMTTIDGVIEKYVPGDYEFNLDQHYLDLYAKKDYGR
                     YGVNGTLPVCFIGANHTTGGNSGSPAIDAKGNLIGINFDRVWEGTMSDVNYDASICRN
                     IMVDIRYVLWVIDKYAGAGHLIDEMKIVGKSK"
     gene            complement(12647..12856)
                     /locus_tag="PLE23_04235"
     CDS             complement(12647..12856)
                     /locus_tag="PLE23_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82505.1"
                     /translation="MKIIKSLIVVSVLSFGFVACNSEAASTEATEATATETPAAEAAP
                     AAEAAAPAAEAAAPAADSTAAAPAQ"
BASE COUNT         3514 a         2753 c         2560 g         4074 t
ORIGIN      
        1 ttttctttag caaagcgtaa tagctttggt aagtggtgta attctgcaat acatctaaaa
       61 tgaaattttc ctcaatggct tgtttcatgg agtagtagat tttgtcaatg tgttccggca
      121 aaaattctac tcgtttttta ccaagtgact tccgcatttt gctgtaaaaa tcttcggtag
      181 tggcgtttat cagttgcact ttgcccttac gatgtttgct ctttctgtta gtaattaaga
      241 aaatgtaagt cggaatgccc gtgttgtaaa acatatcatt aggcaaggca atgatggctt
      301 ccaaataatc gttttcaatg atgtatttgc gtatttcgct ttctccttgt cctgcatcac
      361 ctgtaaacaa agccgaacca ttgtgaacag atgcaatacg gctgccgagt tcggtgtctt
      421 tcattttact cagcatgtgc aatacaaaca tcaactgtcc gtcattcaca cggctggtta
      481 cggcttcttc tttgtagctg cctttcagca taaagcgttt gtcgatgatg gctcctttcg
      541 gtccaacgcc taatgctttt ttatcaggtg cccaggtctt tccataaggc ggattggtaa
      601 gcatgaagtc aaactttaga tccttgtcaa atccataagc actcaatgtg ctgccataag
      661 caattttgtc ggggtcttcg ccttttatca gcatatcgct tttgcaagtc gcataaatga
      721 aaggcgtgct ttcctgtccg tataaatgaa tggtggcgtt gcttttaatc agtccatcag
      781 gattgagcaa aaagtttttg gcaatggtga gcattccacc gctacccaca caagggtcgt
      841 aaatcaaata cgtgccttgc ttgattttgt ccttcaccgg caagaaaaca aggtgcgtca
      901 tcaaatcaat gatttccctt ggcgtaaagt gttctcctgc ttcttcgttg ttttcttcgt
      961 taaatcgtct taccaaatct tcatacacat aacccattgc caagggtggc aacttgtcgg
     1021 ggtgtaattc cactttcgga gaacagaatt tttcaatcag tgaaaacaga atatcagctt
     1081 cgtccaaggt ttcaatttca tttctgaatt tgaattgctt gatgatgtcc tgcacatttt
     1141 cagaaaagcc attcaaatag ttttcaaaat tgcttctcag gtttttcggg tcgtcaagca
     1201 gtttttttca ggtaaacttt gaggtattgt aaaacgccaa gcctgaacca tgtttgctgt
     1261 tcgtcagcaa gctgtctaag ttttggagtt tgtctctgta ttcattgaaa gtctttaaac
     1321 ctgttctttg gtgtgttcta aaagcaagtc cagtctgcga ataactacca tgggcaggat
     1381 aacatctttg tatttccctt tctggtaggt gtttaccaaa acatcatccg caactgtcca
     1441 aagaaagttg ataatgggtt gcagtgattg tgtatttaag ctcattttcg tctttatatt
     1501 ttcgttttta tcaggattct aaattacaat tttaagttgc ataaacgtct cttgtgcggt
     1561 aaaaaaaaat gactcaattg gttttactca cggtataatt attcttaatt gtgttcgtga
     1621 atacttcgta tttcaaaata ttttgtggtt tggggtaaaa attaaaacac agaaaggaag
     1681 gcaatgagtg tcggcttgct cgttgccctt tcaaaatatt atctgtattt tgttcacttg
     1741 tcaaaatggt taggttaagt tttacaattt caatcttgct cgtcagtccg ttgttagtac
     1801 ggtcgttata ccattggcta tacccaatag gatgtacgta tggttgaaga aacagataac
     1861 tcgtcctcgc tttgtattga atatcctgag tacttacatc aacggatgat caatattttg
     1921 cgaatgtctg ccaccttgcc attgtctcta actaccatgg tatataagcc tgcctgtagt
     1981 tgggtatcaa tcttcatact cctaccgctg tagtttgtct tgaaaacctg tgtacccaaa
     2041 taattgaaca cttcgatgtc ggcatgctta aaatcaccat gctgcacttc aaaactaccg
     2101 gaagatgggt ttggaaaaat caaaataccg gcatcttttt ccggagatgg agtagaaagt
     2161 gttataccac acgcagctac actttgacca ctaatcaggg cgtctgaaag acaattgagg
     2221 taacactcaa gattggtata accttccacg ccggtgatag caagtgataa ctcagtaccg
     2281 ttgtgatcct gtacatccgg attaagccca tgggcagcct cccaataatc tggcatccca
     2341 tcgttgtctt tatcagacgg tgactccggt ggattaggat tgacgatgtg tccatcaaaa
     2401 ttataatctg ttccattgac aggatttggg tctatagaaa gttggtcaac gtgcgacatc
     2461 agtctgttat ccatctgatc tcttgggaat gcacccacat tatctgccat atatgcaggg
     2521 agagattcgg tctgcagata ggtgacctcc ggaaaattgt gtctggcagt aagcttttga
     2581 gcagctccga aatcagtatt cgggttggca cccggagcat taaaatcatt acagcaataa
     2641 caaagctggt aatcagaata attggggtaa atattcattt tatttcccga gccaaaaatg
     2701 ctgttctgtg cgaactggat aaggcggatt tccatcatac cgaaaggaaa atcaggcctg
     2761 acgtacatat aattattaac ccagttaagg tcaaggaaaa atttattttc ttctgcagaa
     2821 gggtcaatat tgggtccgta ccaaatgttg ttttgtggat cccaaataag gttgttcgat
     2881 aattcgagtt ttaatttggc tgctgcacaa tagggcgatt cgcagattat ttcagggagc
     2941 ctggcggcaa tacggttcca ggtattgtga tggatgctta atctgtcttg gggatgttcg
     3001 ggtgtagaat agttaatcag catgcctcct ctgtcataat ggtcacctac ggtttctgac
     3061 agaatacagt tctgcattgt cacgtccttt gattgtgata tctggacggc ttcatctctg
     3121 gcattggcaa acgaacaatg atcgatgata atattgcttg caccgtcaag tcgcagcgca
     3181 tcatctaaaa tataatggct ggcgggtacc agatccgggt cgtgtgggcg tgaacgaaga
     3241 tgtcgcagta taatgttatc acctgtacca tcgggttcgt acacttcatc caccaaaaaa
     3301 ccacgcgtga tgataccacc cggtgaagtc tggccggcta tggtgacatt accgtaaacc
     3361 aattctgccg ctgcatctat cagaccactt accttaaaaa gtatatacct cgggccttcc
     3421 tgtgcgagtg catcattgag actcccgggg ccattggcat tcagattggt gacatagatg
     3481 acctttcctc ctcggccacc tgttgccttg gcaccgaatc cttgtgcacc tgggaaagaa
     3541 ggaatatcct gagcagtgac cacaactgtg aaacacaata aacatacctt aagaaaaata
     3601 agccggatca ttataagaca gattttaaga tgggtaaaaa actcactaat tacattgcca
     3661 ctgcaatata agtatttcat aaaatattta caaaatcttg ttttgttttg aacaaaaaaa
     3721 tctgctaccg gaaaaagtgg aggataccgg gctcgaaccg gtgacccctt gcctgccagg
     3781 caagtactct agccaactga gctaatcccc ccttaattat acctttcagc gaatattgct
     3841 gaagcttgtg cgaaatgcga atgttcaaaa ccacaagcaa taatttaaca agatgcaaat
     3901 ctaaagaata atcattgaat cgaaaaatat ttattcgctg agaaaatact cttcatcagt
     3961 ttttaacgat tttccttaaa attgttcctt gctctgtttc aaattttacc aaatatgtag
     4021 cagcaggcca tttgctcgta ccgatttcta ttttgccgga ggcttcacta tcatattttt
     4081 tattaaatat taactctccg ctcaatgttt ggatatcaac cgtacagtta cctgcattct
     4141 gtgatgttat gtgcaatatt tcattcatgg gattaggaaa aatttcgaca gaaatgccgg
     4201 gaagctgatc aactgccgta aggacttgta gatcggaaga ataaacatca ccatgaataa
     4261 tatttgcttc ttcgagtaat gctccgatgc ttgctttcac cacattgccc ataaaccaca
     4321 tattgaggct gtcaacacgg tcgttggctg tatggtaatt tttatttctg aaattggcac
     4381 cgtctgtgag cataagcgcc ggtttgcctg ctgtccagaa acttgcatgg tcgctacgca
     4441 aaaggtcagg tgtcaaggcg ccattacccg gcacttcgag tgtcactacc ttcagttctg
     4501 gaacataagt agtagccgat ttctcaaagg catcgcttat tcctttcgag ttgctattcc
     4561 tcacattggt gataaaattg cccttgtatg aatctgcagc gatggctgct gactgtgccg
     4621 gaaacaaaag attgaacccg ttaggcaatg tctgcgaatt gggcttatct gaataatagc
     4681 ctatcatttc aaaattaagt acaccttcga tggtatcctt taccgaaagt cctccctgac
     4741 cgacatacgc tttactacct atcagaccat cctcttctac atcaaatccg atgaaccgga
     4801 tacttttcct gaaatggtag tctgcaataa tacgtgatat ctccatcata ccggctactc
     4861 cggtggcatt atcatcggca cccggaccaa aaggaacaca gtcgtagtgg gcatcattga
     4921 tgagtattct acccggagat gatgcaccgt aatggtctgc gatgtagttt ttcagctgat
     4981 aatcaccaaa cttagctgta tggatactcg gctcatagtg caaggaggta aaaaggcttt
     5041 caataatatc ttgtaccgcc ttgagttttg ctgctccggc ggtacggtgc ctggtgcctt
     5101 caagccattt cagatctgaa taaaggtgca aggtatccac tctgtccact atagcttgga
     5161 gatcaaagct atgtccatcg taggctacga tatttattct atactctccg tctgaaggta
     5221 gcggcacact tatggcttta gtacctgtac ttatattatt gccaatatca cctgtaatac
     5281 cattgactgt taggggttca aaaccggatc ctgatttttt aaagacatga agttcgacgg
     5341 tacaaacatc gttttcgtca tcttcaagcg taaaattgta ggtaacagtc ttagcattct
     5401 gagcagatga gacattcaaa atggcaggtg gttggttctg ggcaaaaagc tgaacacaaa
     5461 aaatactaaa aatgcccaat agtgtgtttt tcattttcct ggagttttga taaatttgaa
     5521 tctgtgtttg aaaatcaaat ttacgctcca aaaataatat atctgagaat attccagcta
     5581 aaatttaatc cagtacagga gtgactttaa aaccttgggc tttcaacaaa tgaatcacac
     5641 cattttcgcc tcccagatgc ccggcaccta ctgctataaa ggaaggttgg gtttgtgcaa
     5701 tgttgacaat cttttgtatc cagttttggt ttctttttac cagcaataca tcttccatcg
     5761 ccgatccttc tgattggcct ttaatgatag cgtaaagttc gtcaagattc tggcttttat
     5821 atacttgcac cattttctga aaatcgctat cgtctgtctt tacatcaagc atttttacaa
     5881 gttcttttgc ctggaaagaa tatggaagca catcaagaaa gcccatttgt tcttggaggg
     5941 tttcgagtcc tgccatcttg attcctttct tcttagcttt ttccatcagt tcgagttcat
     6001 aagattttgt ttcttcgccc tctccaggtg tcatgagcat ggtagataga aagatgggtt
     6061 tgaagctttt cataaaatca aggcctatac caagtgttga taaaaaccgt ttgtcgagtt
     6121 tgtcgtattc ttctttggta agcaacatgt ctagggtagt atcacctttc atctgcatat
     6181 tggccattac ttcaaggctc atacctgtga tgtcgctcag gtccatctca cctacaagta
     6241 gttcgcagct gttgaaagcc gcatccaaac cctttggttc gaaataatcg ctggccggga
     6301 tgagatggat agtcccaaaa agataggatg gtttcttgat gtccttgcct tcaattttcc
     6361 ataacaaaga agactgtgct atggagggca atgtaaaaag caatgcaaaa agtgcagtaa
     6421 agtaacgcat aggttgtcag aaatttattg aatgaatata accagattgt ttgagaaaat
     6481 gttttaaata ttacaaaatc atgccttacc aacatttgcc ataaagatcg taaccttctt
     6541 tctctagccg tgcttgatgg tcggggtgag caatttttat taattctttt accctttggg
     6601 caatattttt accgtatagc tctgctacac cattctccgt taccacataa tgtacgtgtg
     6661 cccgagaagt gacaacacca gccccggttt ttaatgtagg tactatcttt gacacacctt
     6721 ttttagtaac tgatgctatg gcaataatcg gtttgccacc ttcgctgagt gaagctcctc
     6781 ttataaagtc catctgtccg cctactccgc tataaataga tgtaccgata gaatccgagc
     6841 atacctgacc tgtgagatca atttcaatag cactgttgat ggctgtcatt tttggatttt
     6901 tgcgaatgat ggatgaatca ttaacataag cagcatcaag catcaccaca gcaggattat
     6961 cgtgaataaa atcgtagagt ttttgtgtac caatggcaaa tgaccctact gtctttcccg
     7021 gatatttgtg tttgtacttg ttagtaatta cacctttttc tatcaatggc agaattccgt
     7081 cactaaacat ctcggtatga atgcccaggt ctttatggtt aaccaggaag cgagcgactg
     7141 cattgggtat ggcaccgata ccaagctgca gggtagcgcg gtcttcaact aagttagcta
     7201 tatgtttacc gattgcaagt tcgttgtcac tcaatgaagg tagctgagaa gtatgtatat
     7261 ctgcttcact atataccata gcatcgaacc gacttgcatg tatcagtcca tcgcctaacg
     7321 acctcggcat cttcggatta accactgcga tgattttttt tgctacatcg gtagctgcct
     7381 tggtaacatc aaccgacact cccagagagc aaaatccgtg caaatcgggt ggtgacacat
     7441 gcaccatggc tacatctaaa ggcataaccc ctcgccgaaa caacaatggt acctcgctca
     7501 gaaagatcgg tatatactgt acatgactgt gactgatatg tggtctgatg ttacctccta
     7561 caaaaaaggc attagtccgg aatgcaccat cgtattgagg tagcacataa ggtgccggac
     7621 cttctgtatg aatgtggcat atttcgacat tctgaagctc atcctttctc tccgaaagag
     7681 cgtcgattaa aatattaggc gtagcagccc caccctgtat aaaaacacgg tcatcacttt
     7741 ttacgataga tactgcctgt ccgggcgttt tgaaatcaaa catcacttat tagtttttta
     7801 ttggtttgta ttgctgtgtg agctcctcgt caaacatttt ataaattcgc ttgtattcat
     7861 ctgtccagct ggttgcttct tgaaagccgt gatcttctac cggaaaaact gccatctccc
     7921 agttgtcttt ctttaactct ataagccttt gggccaacct taccacatct tggtaatgaa
     7981 cgtttgtatc gaccatacca tgtaacatga gcaatctgcc tctcaggttt ttagcaaaat
     8041 atatgggcga acttcgtctg aatgctacac tatcttcggt aggtgtattg aggatattgg
     8101 atgtatattc atgattgtaa tgtgcccagt cggttacaga ccgtagtgca gcaccgcact
     8161 taaaaacacc aggttttgta aacattgcca tcaatgtcat aaaaccgcca taagagccgc
     8221 cgtatatgcc aatgcgatct ttgtcaagct gtaagctgtc aactaagtat tttgccccat
     8281 cgacctggtc atcgagatcg cgaccaccca tgtaacggta tatggcagta cgccagtcgc
     8341 gtccataacc ttccgatgcc ctatagtcta tatcaataac tgtatatccg ttgtcagcta
     8401 gcatattatg aaacatgtat tcgcggtaat aggtgctcca ccatttgtgc acgttctgca
     8461 ggtatccggc tccgtgtaca aatactacac ctgctccatt aggtcgctct ggcttgtaca
     8521 ggcgtgccgg tacttttact ccgtccgatg ccggaatcat gactattgca ggttctctcc
     8581 atttataatt gttaaaagat gctgtaatag aagatgtaat tttctgcatt tttgcaccag
     8641 gcttgttctc catcaggtag agctcccaag gcttgttggt atatgaatac cttatggcca
     8701 gatacttttc ttcgggcgat acgcttactt catgattacc tgttagttgg gtaatctgct
     8761 tcatttcacc accattgaca tataatgtat agaaatggtg ttcgaaggga gtaggtttgt
     8821 tggctgtgat gaaaaaattc ttcctatcct ttgacagacg cgcttcgagt atctcaaaat
     8881 cacccgatgt aagtgctttc ttttctagtg tttctgtgtt ttgtgtataa atgtgtgaat
     8941 atcccgtcac ttcagactgg taccagactg tctgatcgtt tatccaacct atccggcccg
     9001 gggcgtccat ccattctact atacccggcc ctccgatcca ggcatcatca tgttgtctgt
     9061 caagaactgt gaatgggttt tcttttgaaa gatcgatcaa tactatccac ctgtctttgt
     9121 ggtcatgagc cttcacttca aatattgctt gcccggtatt gttatagaca ggcccgaaat
     9181 aattgacctc acgtggagtt ttaaacacat cggtataaat agaatcttta ttataatcac
     9241 gatagtatga tggtttgtcg aaaatgcccg gcaatattga acggtcgaac gcaatgaccg
     9301 tgtctttctt aatatcaaaa attagtagtt cgtaactgct ttgcttggag ccgactttat
     9361 tgcgtgtatc caggtcacgt gtatagcctg aaggattgac gaagtccggt actacaggca
     9421 ttttattgac agccggtcgt tcgagttgaa aacagactga cttcatggtc ggatcagcca
     9481 ctatggcagt catatttttt tcaccataat aaaattcctt tgctcgatat atctttagag
     9541 aatcatcgag tgctttgttc attttagcga tgtcacgccg ttggcgcaat acttcgaaaa
     9601 gttcaaactg ttgtttttcg agatagcttt cttgtgcgtt tctttttcca tcatgccttt
     9661 tttcaccttt gataaaattg gtcaactgcc tgagtgcgcc ggtattttta ttccacgaaa
     9721 aaacatttcc ataacttaaa taaagaatat attgtccgtc agcagaaaaa acgggctggt
     9781 attcaccgac ataggtatcc gtcacctgtt tcatttgccc actcgcgaca tcgtaaatgt
     9841 aaatatcacc attcttgcta aataccttcc atttcccggt agggtcatat tcgcctccat
     9901 cagaaaacat atctcgcaat gcagcaatgc tcattttttc ggttttgcct gtcttgaggt
     9961 tgtatgcata cttttcacgt accggtttta tctccggatt ccagtcaaag taaatattct
    10021 taccatctat agaccaggaa atattctccg gtgaataacc cgtaaacttt tcaccttgca
    10081 ttatttgatc aatggataat gcagaaatat tgtgctgtgc cttcgctgat gccgaagcaa
    10141 atattattat aacaagtgca ctcaatattt tcaaatatat cttcatttca tgaggtttgt
    10201 atcatctact taattaacat gacagaatga ctctgaattt gtttttgcat ccatcaaaca
    10261 atttaagaaa gtaaatggtt gtagaattct ttctgccatc taaataccaa aaagcacaga
    10321 agtaccttac tgtaaagtag ccgatggtta gaaaaatttt tagctaagtt atatcgcaat
    10381 aattattttg acttacctac gattttcatt tcatcaataa ggtggccggc gcctgcatat
    10441 ttgtcaatca cccaaagtac atacctgatg tcaaccataa tattgcgaca aatgcttgca
    10501 tcataattga catcactcat tgtcccttcc catacgcggt cgaaattgat accaatcagg
    10561 ttgcctttgg catcgatagc agggctaccc gaattgccgc ctgtagtatg gttggcacca
    10621 ataaaacaaa ccggcaatgt accattcaca ccataacgac catagtcttt cttggcataa
    10681 agatcgagat agtgctggtc gaggttaaat tcgtaatctc cgggcacata tttttcaata
    10741 actccgtcaa tggtggtcat gatgtcatat tttatgccat cttttggtga atatggtttt
    10801 acttttccgt aagaaaccct gagcgtactg tttgcgtccg gataaaattt cttttcctta
    10861 aacacttcca tctgtgcctg catgtatgtt cgctgcaagg tgtttatttc ggcagaatag
    10921 gtattggcct ttggtgaaat tgaattttgg ttaaattcgt acacttcttt cagcagtgcc
    10981 accagggcat cttcgcgcaa tagtttgaca gcttcagtag caggtagggc caagatagta
    11041 ttcattttgg cttcgttgat aaggtttgac gaatcaaaca ttgaagcaaa ctgttctttg
    11101 ctaagcttag tgatagtggc aggctgcaga cctgactcca catttgcctt gtagatgaga
    11161 atcagttcaa caaacatttc cttgtctata tcagcttcaa aatttttata aaaacctgcc
    11221 agcccatccc gagcttttgt tgcttgtttt gaatattctg tctcaccctg gctatcaaaa
    11281 gcctttataa gttgtatagc ctgattgccc atacgagtaa aatcaagaat ccttgaaaac
    11341 acttcggcta ttgcttctct tgctacagca tagcgcttgt attcggagta ttttttttca
    11401 aattcaggta aaagttgtcc gtatttttga ttcagtgcag ggtcggcatt tattcttttc
    11461 gtaaaatcgg cttcgtactt cattttgcgg ccgagaccat cggttttttt cacaccgagg
    11521 ctctccccta tccatttttt ccacgcatta gctattgagg catactttga tgcatatttt
    11581 attttcgtag cagggtttgt tcgcattttg gcgtccatta tcttgagtgc cttttcgcgc
    11641 agacttatcc ttaccggatt aagtacttcg gtggcttgct gtacgcctat acctggaaga
    11701 tactcgtctg tacggcccgg aaacccaaag atcatggtga agtcgttttc cttcacacca
    11761 tcaagtgata tagggaaata atgtttagga acataaggaa cgttatctgg cgaataatct
    11821 gcaggaagat tgtttttgcc ggcataaatt ctgaatactg aaaagtcacc tgtgtgcctt
    11881 ggccaaaccc agttatcagt atcggagcca tatttgccga tactttcagg tggtgcccct
    11941 accattcgca catctttata ggtaaccgag ataatggcaa aatactgatt gcctgtaaaa
    12001 aagggtttaa tgcttacagc ttcataagcc tgaatgacga gagatttcga tacttttgca
    12061 atattctggt ctatgactga ctgtctttct gattcggtca ttttatcatt tacacctagc
    12121 aacacttgtg ttgatacatc ttcgatacga gatataaaag tgactgtaag accctcacag
    12181 ggcaattctt ccgacatatt ttttgcccag aacccatcac ggatataatt atgctctaat
    12241 gtagaatgtt tttgaatctg gccatatccg caatgatgat ttgtaagcaa aagtcctttc
    12301 ggtgatacga cttcgcccgt acagccacca ccaaaatgaa gtactgcatc ctttagcgag
    12361 cctttgttga tgctgtaaat atcctccgcc cggagtttca tgccagccgc acgcatttcc
    12421 ttttcattca gctgtttgag gaatataggt agccacattc cctcatcggc gtagctgtat
    12481 atttttacca atagcaacaa tagtactgca aaaactatcc ttttcatcgt ttattttttt
    12541 gtaaaaatat tgattaaagc ctaattgtca tagaaagctt aaaaaatagc acaaaaaaag
    12601 cctgccggaa taccgacagg ccttttatct taagataaac tcttaattac tgagcaggag
    12661 cagcagcagt tgagtcagca gcaggagcag cagcctcagc agcaggagca gcagcctcag
    12721 cagcaggagc agcttcagca gctggtgttt cagtagcagt agcttcagtt gcttcagtag
    12781 atgcagcttc gctgttgcaa gctacaaaac cgaaagaaag aacagaaaca acgataagag
    12841 atttgatgat tttcatttta aaagaaattt gatgttaaaa aaaaattttt taaattaatc
    12901 t
//
LOCUS       DAOQQY010000051        12837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1679_length_12837_cov_6.335863, whole genome shotgun sequence.
ACCESSION   DAOQQY010000051 DAOQQY010000000
VERSION     DAOQQY010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12837
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1679_length_12837_cov_6.335863"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLE23_04240"
     CDS             complement(<1..510)
                     /locus_tag="PLE23_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HMX82506.1"
                     /translation="MCGIVAYIGNKPAYPIMIKGLHRLEYRGYDSAGIALLNGSLTII
                     RRKGKVKELEDFAEGQNLVGTVGIGHTRWATHGKPDDRNAHPHLSGNGRLTLVHNGII
                     ENYDTLKKALEKLGHEFTSETDTEVLVHLIEELQSRDNLPIEEAVRVALQKVVGAYAI
                     VVIDKEDPEK"
     gene            complement(530..1882)
                     /locus_tag="PLE23_04245"
     CDS             complement(530..1882)
                     /locus_tag="PLE23_04245"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 family protein"
                     /protein_id="HMX82507.1"
                     /translation="MKLGNLLTLVTIVSLLAVSCNDPLFLGSDLLKQDQVGLGYTDTI
                     SMNTTTIRQDTFQVYNPSVKLIDAFLVGNYNDTHFGKTKATTYLQYRLGTTQFPDFTN
                     TKFDSLVLGMVYDTLKVYGDYAKETTLNVFALNESMVSTSSYVSDKSFNADPSPIGTL
                     TFIPNPTQKISFTEYHSGVKDTINTNQMRIRLSDQLGMQLMSAPDSVYKTNDNFLKFF
                     KGIKISAQSENNGMLSFLPLDLRSKISLFYSRNDTSFQYDFIVSSLSARTVAFEHDYA
                     GAFVEKYINSSNKSDSLAFLQGMQGTLVKVEMPYITALKDKIVNKAELNIYVKEFAGE
                     SPLFRSVANVLLSEKKDSGKFVSINDVLVAISVGGESGLSKFFGGTLVEESVNGQKRL
                     KYKMDISSHIQKMIKGSVDQSLYLTVYRRGESANRVALYGTGSVLPPQLKITFTDSIQ
                     "
     gene            complement(1912..2730)
                     /locus_tag="PLE23_04250"
     CDS             complement(1912..2730)
                     /locus_tag="PLE23_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HMX82508.1"
                     /translation="MSKKRLLIVTQELSPYTEDTLISELAGKLPLYLNEKGYEIRILM
                     PKFGSINERRHRLHEVVRLSGMNIIVDDDDYPLIIKVASLPGARLQVYFLDNDEFFKR
                     KSMFTDDDGNAYTDNTDRMVFFCKGVIETVRKFGWSPDVIHCHGWMTSLIPAFIRSGY
                     KNDPVFSSSTLVYTAYPHEMDNALQATFMDKALISHIEKEDLEGYFTNDQLLVDKGAM
                     QFSDAVVKGSAELSEDVLKEIEKNGKPILDFSEENYPASHAEFYKQLLAQIATS"
     gene            2871..3725
                     /gene="panC"
                     /locus_tag="PLE23_04255"
     CDS             2871..3725
                     /gene="panC"
                     /locus_tag="PLE23_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HMX82509.1"
                     /translation="MLIIHTVEALQKIINEKKSSGLTGFIPTMGALHSGHISLIKQSK
                     SETDYTICSIFVNPTQFNDPNDLKKYPRPVAHDIEILEDANCDVLFLPEVDEIYPNGS
                     SDKKKFDFGQLDKVMEGFFRPGHFDGMAQVVDRLLEILQPDKLFMGQKDFQQLTIVRA
                     MLRLTGRNTELVTGATFREKDGLAKSSRNIRLTSEHRKEATLIYKTLKWAKKNASSLN
                     FDDISLKAMKKLTRTDFKPEYFDIVDGITLQKLNSIQDSDYIVACVACWVGEVRLIDN
                     IIIKDETE"
     gene            3741..4352
                     /locus_tag="PLE23_04260"
     CDS             3741..4352
                     /locus_tag="PLE23_04260"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMX82510.1"
                     /translation="MLYPFLQKGRAEAGVDEAGRGCLAGPVVAAAVILPEDFAHPLLN
                     DSKKLKASDRYALRQIIEHEAVAWAVSFIDPDVIDEVNILNATFLAMNEAVNKLKIRP
                     QHLLIDGNRFKDTTAGIGYSCIVKGDALYASIAAASILAKTYRDDQMLTLHQQHHQYL
                     WNNNKGYPTTDHRLAIEVHGITIHHRKSFRLLPEEPQLRLFHE"
     gene            complement(4361..5410)
                     /locus_tag="PLE23_04265"
     CDS             complement(4361..5410)
                     /locus_tag="PLE23_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMX82511.1"
                     /translation="MNASLVKINQTLLFIILTGLILYFGKPLLMPLSFSLLIACVLYP
                     FSKWLELHRFPKVLAITISLSMLGVLLVIIGALFVRIIAAFTLKWPLLEKKLLTLINT
                     ISEFFISDLQISFVQQELWLSNALDKLPEFILPILKNVLYESSVGLVLIILIPIFSGL
                     ILYYRRRLVNSLFSLFPTVGEEIITQIIRKTIDTYYNFIKGMLLVYLIVGALNSVGLL
                     IIGIPDAFVYGFLVAIMTFIPYIGIIIAAALPVSVAWITFENPVYPFMVILLFTIVQY
                     LEANVIFPFAVSSKLNVNALATLAMILLGGMFWGGSGMILFVPFAAIIKIVADNLKAN
                     NVISILFGEDKKLDR"
     gene            complement(5436..6245)
                     /locus_tag="PLE23_04270"
     CDS             complement(5436..6245)
                     /locus_tag="PLE23_04270"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMX82512.1"
                     /translation="MKTLGLEKICTDAADLIKIVALYIKSETGQVTEDKIEHKSFNSL
                     VSHVDQNAEKMLINGLQSIVPGSGIIAEESEHADADAESVWIIDPLDGTTNFLHGIPF
                     FAISVALTVRGKCVIGIVYDVIHDQCFYAWDEGGAYMNDQKISVSRHSELSNCLAATG
                     FPYYDYTPIESYIRCLRYFMKKTRGIRRIGSAALDLAYVACGRFDFFFEYGLNNWDIA
                     AGSILVKEAGGTVTDINGGDNYLCNGSILASNGFVHQKTLHIIKPEFLSYI"
     gene            complement(6329..9016)
                     /locus_tag="PLE23_04275"
     CDS             complement(6329..9016)
                     /locus_tag="PLE23_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HMX82513.1"
                     /translation="MPPVVKKIIARTILVFSSVFLLSVILTAFFEDKIGRLIIAELNK
                     NITTELKVEDVSLSLIRAFPKVSATLKNVRLADTGKKNLLSIGELSFSMGIWSAFTGK
                     PSISKIKLKDGVINIVIDNKGKPNYEIFKKNTDNKSQNDVSFSISKAIFDNFAITYTN
                     QLKQSDYKATITKGTLAGEFSADEFILDLDTDWILNSIMHENSNYLQEKPLSLTGKFD
                     INSKKGTYKIKECLLNLASNNFTVEGSVNEGKNGTDFDLFALGKDINLESVFNLGISQ
                     LNDAAVRFKSKGIIQVNARIDGTLNASSNPAILVQYNLKNGSVQGKDVGGMIDQISFT
                     GSFSNGWSHNFRTSSFSLDNCKASFNGLPFSLNLKLRNFEKPDIDLSANGKIPLERVY
                     AFIAQAKGGAGFINVKNVVIKGNMADMRSDQGATRVKASGSILFEDAVLNYRGEDLKF
                     STGEINFSNNFINLTKVDLRGLDSDLQIDGRISDYLPFIFSKDPNDKLGLGINIHSNY
                     INLERWMTMLSDKDEVQTKTTSSTPVNSGNPFDKIQGTIKADLDVIKHKKIMARNFSG
                     DLIFEGGDMLVSGDVQAMEGDWNIEGQLNFDNTGSHLKATLESSKVNIKEFFAQSDNF
                     GQETLTDNNLSGRLNSRILILADWDKNGKFDINKLHVYGSMSVDNGEMRAFKLLESFS
                     AFIKMKDLRNIRFVNLKNLIEIEDGIIHIPAMFIQSNAANLTVSGQHTFNNDIEYNFK
                     INAGQVLLNKLNLVNKTSDALPAQKGGFFNLYYNLKGTTEKFVNKRDKSLVKSNFTKS
                     EIRKHKIMNTLLAEFGSMPEFDEPDGWSDQGENPKSASFTNNNSFNTGNNTALRPSLK
                     STLEEAKQTLKNPTKAVVKPEKKTFRDEEDEEVEYLDFK"
     gene            9160..9234
                     /locus_tag="PLE23_04280"
     tRNA            9160..9234
                     /locus_tag="PLE23_04280"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9195..9197,aa:Val,seq:tac)
     gene            9473..11047
                     /locus_tag="PLE23_04285"
     CDS             9473..11047
                     /locus_tag="PLE23_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82514.1"
                     /translation="MRILLLFIFSFLLTSHTNAQESSKPLSVKWGNELKSPSGTFIYK
                     IVQNDLNGITVLRLKKSGMFSTSQKIIFERYNAGFGIEKSKETTLKYKNKSMDFEQAF
                     SLGGKLYLFSSFANQKDQKKYLFGQEVRATSLTPEKKLSYLDEMSFTLLNDYAGFQFD
                     ISPDSSLLLVYHAIPGKKEEPAKYSLLVVDKELNKLWSTQYTLQGKLKDHYIENIKLD
                     NNGNAFLLLSTNIGTGTQKLQDDKYRMISIRDQGQKIDEIDIGVSDIYINGLNFKVLK
                     DNNIVLAGFYSNRGVNSVKGVVYYVINGDTKQIIHQSTKEFGVDFVTSNLSESKRNKL
                     KDSTRKTALPELPKFNLDHLILRGDGGALLLAEQYDYSVYSYYDYFSRTYTNRYVYMY
                     DDIVAVSINPEGNIDWATRVPKHQETMDDEGIYSSYCYAVSGDKLVFLYNDNPRNYNR
                     DQKSRARYEFDAYNGMPAYAIIESDGTLHTGLLENGTDDKQFIRPSICKQTALSKMLI
                     YKEYNGKYKWGEVMVK"
     gene            11197..>12837
                     /locus_tag="PLE23_04290"
     CDS             11197..>12837
                     /locus_tag="PLE23_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HMX82515.1"
                     /translation="MYKEFKGLNLPEIDKEILQFWKSENIFEKSIDTKDASNPYVFYE
                     GPPSANGMPGIHHVMARTVKDLFCRFHTMLGQRVERKGGWDTHGLPIELSVEKELSIT
                     KEDIGKSISVDEYNHKCRETVMRYKKEWDNITEQMGYWVDLDNPYITFENNYIESVWH
                     LLQLLYNKGLLYKGYTIQPYSPAAGTGLSTHELNQPGCYREVTDTSVVAMFKVIKDER
                     AQFLFDDEDEDVRIIAWTTTPWTLPSNTALTVGSKIEYVKIKTINPYTKQAFSAVLAN
                     ELVNSWFGEKKAEILSKSDVFSGEKLKGISYEQLLPFAQPEDGDAFKVITGDFVTTQD
                     GTGIVHTAPSFGADDMRVARANGIGSLTLVDKQGRFTAEAGEFARRYVKDYKDDPNYV
                     NVDIDIAVKLKQENKAFNVQKFVHNYPHCWRTDKPVLYYPLDSWFIKTTAMKDRMVEL
                     NKTINWKPASTGQGRFTNWLENLQDWNLSRSRFWGIPLPVWRTKEADDELCIGSVEEL
                     KKKTEQANKALNLQQSVPTDLHRPYIDEVVLYSEKTGKP"
BASE COUNT         3845 a         2487 c         2613 g         3892 t
ORIGIN      
        1 tttttcgggg tcttctttat caataacgac tatggcataa gcacctacta ctttttgtaa
       61 tgcaactctt acggcttctt ctataggaag attatctctt gactgaagct cttcaataag
      121 atgtaccaat acctccgtgt ctgtttcgct tgtaaattcg tggccgagct tttcaagtgc
      181 ttttttaagc gtatcgtagt tttcgatgat gccattgtgt accagtgtca ggcggccgtt
      241 gccggacaag tgtgggtgtg catttcggtc atccggtttg ccgtgggttg cccatctggt
      301 atgccctatt cctacagtac caacaagatt ttgaccttca gcaaaatcct caagctcttt
      361 aacttttcct ttacggcgta tgatggtcaa ggatccattg agcaaagcga taccggcact
      421 gtcataacca cggtattcaa gacggtgcaa tccctttatc attattggat aagctggttt
      481 attgccaata tatgcaacta ttccgcacat ggtagtagat gttgagaggt tattgaatag
      541 agtctgtaaa tgttatcttc aactgaggcg gaagcacact gccggtcccg tataaagcaa
      601 cacgattggc agactctccg cgtctatata ctgtaaggta aagtgattga tctacactgc
      661 cctttatcat tttctgaata tgtgatgaaa tatccatttt gtatttcaat ctcttttggc
      721 cattaacgga ttcttctaca agtgtgccgc caaaaaactt tgacaatccg gattcaccac
      781 ctaccgaaat ggctaccagt acatcattta ttgagacaaa ttttccgcta tctttttttt
      841 ctgataataa tacatttgct acagacctga acaaaggact ttcgcctgca aattctttta
      901 cataaatatt tagctctgcc ttattgacaa ttttatcttt taatgcggtt atgtaaggca
      961 tctctacctt aaccaaagta ccctgcatac cctgtaagaa ggcaagcgaa tctgatttat
     1021 tgctactatt gatatatttt tctacaaaag cgccggcata atcgtgttcg aatgctacgg
     1081 tacgtgcact aagtgatgaa acgataaaat catattgaaa tgaagtatca ttacgagaat
     1141 aaaagagtga aatttttgat ctgaggtcca gtggaaggaa tgaaagcatc ccgttgtttt
     1201 ctgattgggc acttattttt attcctttga aaaatttcag gaaattgtca ttcgtcttat
     1261 aaacagaatc aggtgcactc atcagctgca tcccgagttg gtcactcaga cgtatgcgca
     1321 tttggttggt gttgatggta tctttgacac cactatgata ttcagtaaaa ctgatctttt
     1381 gggttgggtt agggataaag gtgagggtac cgataggcga agggtctgca ttgaatgatt
     1441 tgtcggacac atacgaagag gtagagacca tactttcatt gagcgcaaat acattgaggg
     1501 tggtctcttt tgcatagtca ccatatactt tgagtgtgtc atataccatc ccgagtacca
     1561 gactgtcgaa ttttgtattg gtaaaatcag gaaattgggt agtgccgagc ctgtactgta
     1621 ggtaggtcgt ggcctttgtt ttgccaaaat gcgtgtcgtt atagttgcct accagaaatg
     1681 catcaatgag tttgactgat gggttgtaaa cctggaatgt gtcctgtcta atggtagttg
     1741 tgttcatact aatagtgtca gtatatccca gacctacctg atcttgcttc agcaaatcgc
     1801 tacctaagaa taatggatcg ttacaggaaa ctgccaatag tgagacaatt gtaacaaggg
     1861 tcagtaaatt ccctaacttc atacggaata ttgataaatt ttattaaaaa atcaagaagt
     1921 agctatctgg gcaagtaact gcttatagaa ttcagcatgg cttgcaggat aattctcttc
     1981 cgaaaaatcc aaaattggct taccgttttt ttcgatttct ttcagaacgt cttcggacag
     2041 ttctgcgctg ccttttacca cagcatccga aaactgcatt gcacctttgt caacaagtaa
     2101 ctgatcattt gtaaaatatc cttcaagatc ttctttttca atgtgcgaaa tgagtgcttt
     2161 gtccatgaag gtagcttgaa gtgcattgtc catttcatgc ggataagccg tatatactag
     2221 agtagaacta ctgaatacag gatcgttctt gtagccgctg cgtatgaaag ccggtatcag
     2281 cgatgtcatc caaccatggc aatggataac atctggcgac caaccaaact ttctgacagt
     2341 ttcgatgaca cccttacaga agaataccat ccggtcggta ttgtctgtgt aagcattgcc
     2401 atcatcatcg gtaaacatac tttttctttt gaaaaactca tcattatcaa ggaaataaac
     2461 ctgcaatctt gcaccaggta atgatgccac tttgataatc agtggataat catcatcatc
     2521 tacaatgatg ttcattcctg aaagtcttac tacttcgtgt agtctgtgtc gtctttcatt
     2581 tatggaacca aacttaggca tcagaattcg tatttcatat cctttttcat tcaaatacaa
     2641 aggaagtttt cctgccaatt ctgaaataag ggtgtcttcg gtgtatggtg ataattcttg
     2701 ggttacaatt aacaaacgct ttttactcat tgaaatttat tctgtagata ctttaaataa
     2761 aaaatgccca caaaagtacg aataataatg cagttttttt aaatattgtg cttttattac
     2821 tgaaaattag tggactaaac tataatttta acgctcttta actaaatatt atgctgatca
     2881 ttcatactgt agaggcactg cagaaaataa tcaacgaaaa gaaatcttcg ggacttacag
     2941 gatttatacc aactatgggc gctctgcata gcggacacat ttcacttatc aaacaaagca
     3001 aatccgaaac agattataca atttgctcga tcttcgtcaa ccctactcag ttcaatgacc
     3061 cgaatgatct gaaaaaatat ccacgcccgg tggcacatga tatcgaaatt ctagaagatg
     3121 caaattgtga tgtacttttt ttgcctgagg ttgatgaaat ttatcctaat ggctcgtctg
     3181 acaagaaaaa atttgacttt ggccaacttg acaaagtgat ggagggcttt ttcagaccgg
     3241 gacacttcga cggtatggca caggtcgttg acaggcttct ggagatttta caacccgata
     3301 aattatttat gggccagaaa gactttcaac aacttaccat tgtaagggca atgctccgac
     3361 ttaccggccg caatactgaa ctagtgactg gggccacttt cagagaaaag gatggactcg
     3421 ccaaaagtag ccgaaacata aggttaacat ccgaacatag aaaagaagca acgctcattt
     3481 ataaaacatt gaagtgggca aaaaaaaatg cttcgtcttt gaattttgac gatatttctt
     3541 taaaagctat gaaaaaactt actcgtactg attttaagcc tgaatatttt gatatagtgg
     3601 acggcattac gctccaaaag cttaattcta tacaagacag cgactacatt gttgcatgtg
     3661 ttgcttgctg ggtaggggaa gtacgcctca tagacaatat tattatcaaa gacgagacag
     3721 aataactctt caagcacacc atgttatatc catttctaca aaaaggaaga gcggaggccg
     3781 gtgttgatga ggccggacgt gggtgtcttg caggtccggt ggtggcagct gccgtcatcc
     3841 tgccggaaga ttttgcccat ccattgttga atgattcgaa gaaattgaaa gcttcagacc
     3901 gttatgcgct tcgtcaaatc atagaacatg aggcagtcgc ctgggctgta agttttatag
     3961 acccagatgt aatagatgag gtaaatattc tgaatgccac atttttagcc atgaatgagg
     4021 ccgtaaataa actgaaaatc agaccacaac atttgttgat agacgggaat cgattcaagg
     4081 acactactgc cggtataggc tacagttgca ttgttaaggg cgatgctttg tatgcatcga
     4141 tagctgcggc ttccattttg gctaaaacct accgtgatga tcagatgctg actcttcatc
     4201 agcaacacca tcaatactta tggaacaata acaaaggata tccgactaca gatcaccgac
     4261 tagctataga agtgcatggt attaccattc atcacagaaa gtcattccgc cttctgccag
     4321 aggagcctca gctcaggtta tttcatgaat gagatactgt ttacctgtcc agttttttat
     4381 cttcgccgaa taatatcgaa atcacattat tcgccttgag attgtcagct actattttga
     4441 taatggcagc aaacggtaca aacaatatca tgccgctccc accccagaac ataccaccca
     4501 acagaatcat agcaagtgtt gccagagcat ttacattgag cttgctactc acggcaaaag
     4561 gaaagatcac attggcttca agatactgga cgatggtgaa caatagaatc accataaatg
     4621 ggtaaaccgg attttcgaat gtgatccaag ctactgagac tggcaaggct gctgctatta
     4681 ttatgcctat atatgggatg aaagtcatga tggctacaag aaaaccatat acaaatgcat
     4741 cgggtatgcc tatgataagc aatcctacac tattgagggc acctacaatc agatatacca
     4801 acaacatgcc tttgatgaag ttgtagtaag tatcaatggt tttacgtata atttgggtaa
     4861 tgatttcttc gcccactgtc gggaataaac taaacaaaga attgaccaac cgccgacgat
     4921 aatacaaaat taaacctgaa aatattggta tcaaaataat gagtaccaaa ccaacggacg
     4981 attcgtaaag gacatttttg agtataggta gaatgaattc cggcagtttg tcaagcgcat
     5041 tgctcaacca gagttcttgt tgtacaaaac ttatctgtag gtcggagatg aagaattcgc
     5101 tgatggtatt tatgagtgtg agcagttttt tttcgagtaa tggccatttt aaggtaaatg
     5161 ccgcaataat ccgtacaaaa agtgccccga tgataacaag taatacaccc aacatactga
     5221 gactgatggt tatggcaagg actttcggaa atctatgcag ttcgagccat ttgctgaatg
     5281 gatatagaac acaagctatt agtaatgaaa aactcaaggg catcaaaagg ggtttcccaa
     5341 aatacagtat cagacctgtg aggatgatga agaggagtgt ctggtttatt tttaccaaag
     5401 aagcattcat atcattgaag gtaggttact ttgattcaaa tgtacgataa aaattcgggt
     5461 tttataatat ggagtgtttt ttgatggaca aatccattgc tcgcaagtat tgagccgttg
     5521 cagagataat tgtcacctcc gttaatgtcc gtaacggtgc caccggcttc ttttactaaa
     5581 attgagccag ctgctatatc ccaattatta agaccgtact cgaagaagaa gtcgaatctg
     5641 ccacatgcca cataggccag atcgagtgct gccgagccta tccgccttat gccgcgggtt
     5701 tttttcataa aatatctcag acatctgatg tatgactcta ttggcgtata atcatagtaa
     5761 ggaaagcctg tagcagcaag gcaattggaa agttcgctgt gccgactgac actgattttt
     5821 tgatcattca tgtatgcacc tccttcatcc caggcgtaaa agcattgatc gtgtatgaca
     5881 tcatatacta tacctatcac acatttacct ctgacggtta gtgccacact aatggcaaaa
     5941 aacggtattc catgcaaaaa attggtagtg ccgtcaagtg gatcgatgat ccatactgat
     6001 tcagcatctg catctgcatg ctctgactct tcggcgataa taccacttcc gggcactatg
     6061 ctttgcaatc cgtttataag cattttctct gcattttgat caacatgact taccaggctg
     6121 ttgaatgatt tgtgttcaat cttatcttct gttacctgcc ccgtttccga ctttatgtag
     6181 agggcgacta ttttgattaa atcagctgca tcagtacaaa ttttttccaa tccaagtgtt
     6241 ttcatatatt tgatcagggt ttaatcactt agcatgatgc tgaatatttt taaccggcat
     6301 gccggaatgt tcggttggtg aaagatattt atttaaaatc gagatattct acttcttcat
     6361 cctcttcgtc tctaaatgtc tttttctcag gttttacaac cgccttggtc ggatttttga
     6421 gtgtttgttt tgcttcctca agggtcgatt tcaggctggg gcgcaaggcg gtattgttgc
     6481 cggtgttgaa tgagttgttg tttgtaaaag atgcagattt tgggttttct ccttggtccg
     6541 accatccatc cggttcatca aattcgggca tggagccaaa ttctgcgagc agtgtgttca
     6601 tgattttgtg ttttctgatt tcactttttg taaaatttga tttgacaagt gatttgtcac
     6661 gtttattgac gaatttttcg gttgtccctt tcaggttata gtaaagattg aagaaaccac
     6721 ctttttgtgc cggtaaggca tcagaggttt tgttgaccag attcagtttg ttgagcagaa
     6781 cctgacctgc atttatcttg aagttgtact ctatgtcgtt gttgaaagta tgttgtccgc
     6841 ttacggtaag gttggcggca ttgctttgaa taaacatagc cggtatatgg atgataccgt
     6901 cttctatttc aatcagattt ttaagattga caaatctgat gttgcgcagg tctttcattt
     6961 tgataaaagc cgaaaaactt tccagaagct tgaatgcccg catttcacca ttgtccaccg
     7021 acatgcttcc ataaacgtgt agtttgttta tgtcaaattt tccatttttg tcccagtcag
     7081 ctagtatgag tatccttgaa ttgagcctac cgctcaggtt attatctgta agtgtttcct
     7141 ggccaaaatt atctgattgc gcaaaaaatt cttttatatt gaccttgctg ctctcaagtg
     7201 tagctttcag gtgcgacccc gtattgtcaa aattgagctg accttcgatg ttccagtcac
     7261 cttccattgc ctgtacgtca ccgctcacga gcatatcacc accttcgaat ataagatcgc
     7321 cagaaaaatt gcgagccata atctttttgt gcttaatcac atccagatct gctttgatgg
     7381 ttccctggat tttgtcaaaa ggattcccac tgttaacagg tgttgaagag gtggttttag
     7441 tttgtacttc atctttgtct gagagcattg tcatccaacg ttcgaggttt atatagttgg
     7501 aatgtatgtt aatgcccaga ccaagcttgt catttggatc tttactgaag ataaatggca
     7561 gatagtccga aattctgcca tcaatctgaa ggtcactatc caacccacga agatccacct
     7621 ttgtcagatt gataaaattg ttcgagaagt ttatttcgcc ggtactgaac ttcaggtctt
     7681 cgccacggta gttgagcacg gcatcttcaa ataggattga gccagaagct ttaacgcgtg
     7741 tggcgccttg gtctgatctc atatcggcca tattaccttt gatgaccaca ttttttacat
     7801 taataaaacc ggcgcctccc ttcgcctggg caatgaaagc atacactcgt tcaagcggaa
     7861 ttttcccgtt tgcagatagg tcaatatcgg gtttttcaaa gtttcgtagc ttcagattga
     7921 gagaaaaagg cagtccgttg aagcttgcct tacaattatc cagagaaaaa gatgatgttc
     7981 tgaagttatg tgaccaacca tttgaaaaac tacccgtaaa cgaaatctgg tcaatcattc
     8041 cacctacatc tttgccttga acagagccgt ttttcagatt atactgtaca agtatagcag
     8101 ggttggaact ggcattcagt gtgccatcta ttcgcgcatt cacttgaatg atacctttgc
     8161 ttttgaatct cacagccgca tcgttgagtt gcgatattcc gagattaaag acagattcga
     8221 ggttgatgtc cttacctaat gcaaacaaat cgaagtcggt accgtttttt ccttcattta
     8281 ctgaaccttc aacagtaaaa ttgtttgatg ccaggttaag aaggcattcc tttattttgt
     8341 aggtaccttt tttactgtta atatcaaatt ttccggtcag gctcagaggt ttttcctgca
     8401 gataattcga attttcatgc ataatgctat tcagtatcca gtcagtatcc aggtctaaga
     8461 taaattcgtc agcagaaaat tcgccggcga gggtaccttt agtaatagta gccttgtagt
     8521 cagattgttt gagttggttg gtataggtaa tggcaaagtt atcaaatatt gctttgctta
     8581 tgctaaatga tacatcgttc tggcttttgt tgtcagtatt ttttttaaat atttcgtagt
     8641 ttggtttacc cttattgtct ataacgatgt tgatgacgcc gtccttcagc tttatttttg
     8701 aaatgcttgg tttgccagta aatgcactcc aaatgcccat cgaaaaacta agctctccaa
     8761 tagaaaggag attttttttt ccggtatcag caagccggac atttttgagc gtagctgaca
     8821 ctttggggaa ggcacgtatg agagagagag aaacatcttc taccttcagt tcggtagtaa
     8881 tgtttttatt aagttcagct ataatcaacc gaccgatttt gtcttcaaaa aatgcagtaa
     8941 gaatcaccga taacaggaag acagaagaga ataccagaat agttctggca ataatttttt
     9001 taactactgg aggcataggt tattattgca atacaaaagt aacggtaagt tttgtgatat
     9061 tagtatttag cttaataatt acaataagtt tatttttttt taaaaaaata gttgcataat
     9121 acaaatagta cacttatctt tgcctcgctt ttcgaaaaag ggcgcatagc tcagttggtt
     9181 cagagcatct gccttacaag cagagggtcg gaggttcgaa tccttctgcg cccacacaca
     9241 aggcttcatc gattatatcg gtggagcctt tttggttctt agcaccatgt tgttaaatgc
     9301 acgttgtgca atattttctc acaaaaaatt tatctttgta gtttataatt gaatggcttt
     9361 taaatggtca ggtggcaatg caccatgcat ttaacaaagt tttaatgatt gtcacattac
     9421 acttttactt gcttgttcgt tcttaatcaa taacaatcga ttaatattat acatgagaat
     9481 tctccttctt tttattttta gcttcttgtt gacatcgcat acaaatgcac aagagtcgtc
     9541 aaaaccactt tccgtaaaat ggggaaatga attgaaatcg ccatccggta catttatata
     9601 taaaatcgtc caaaacgacc tgaatggaat tactgtatta aggctgaaaa agagtggtat
     9661 gttcagtact tctcaaaaaa ttatttttga aagatacaat gctggctttg gtatcgaaaa
     9721 aagcaaagaa accacgctta agtacaagaa taaaagtatg gattttgaac aggctttttc
     9781 cttgggtggc aaactgtatt tgttttcgtc ctttgctaat caaaaagatc agaaaaagta
     9841 cctttttggc caggaagttc gtgcgacatc acttacacct gaaaaaaagt tgagttattt
     9901 ggatgagatg tcatttacct tattaaatga ttatgccggc tttcaattcg acatttcacc
     9961 tgatagtagt ttgcttttag tctatcatgc catcccgggt aaaaaggaag aacctgccaa
    10021 atacagcctg ctggtagtgg acaaggagtt gaataaattg tggtcaacac aatacacgct
    10081 acaaggaaag ctcaaggatc attacatcga aaacatcaaa cttgataata atggcaatgc
    10141 ctttctatta ctatccacaa atatcggcac cggtacacaa aagcttcagg atgataaata
    10201 ccgtatgata agcatccgtg atcaaggtca gaaaatagac gaaatcgata ttggcgtaag
    10261 cgacatatat attaatggcc ttaacttcaa agtattgaaa gacaacaata tagtacttgc
    10321 agggttctat agcaaccgtg gtgtaaattc tgtaaaaggt gttgtgtatt atgtcatcaa
    10381 tggcgacaca aagcagatta ttcatcagag tacaaaagag tttggtgtcg acttcgttac
    10441 aagtaacttg tctgaaagca aaagaaacaa acttaaagac agtacacgaa agacagcctt
    10501 acctgaacta ccaaaattta accttgacca tctaatactg agaggtgatg gcggagctct
    10561 tttacttgcc gagcaatatg attacagcgt ctattcgtac tatgactatt tctcacgcac
    10621 atataccaac aggtatgtgt atatgtatga tgatatagta gctgtcagta ttaaccccga
    10681 aggcaatatt gactgggcaa cccgggtgcc aaaacatcaa gaaactatgg atgatgaagg
    10741 tatttattct tcatattgct atgctgtaag tggcgacaaa ctggtatttt tatacaatga
    10801 taatccacgc aactacaacc gcgatcaaaa atcaagagcc agatatgagt ttgatgcata
    10861 taacggaatg ccggcctatg cgataataga aagtgacggc acactccaca ccggccttct
    10921 cgaaaatggc actgatgaca aacaatttat aagaccatct atctgcaagc aaacagcgct
    10981 gagcaagatg ctcatttata aagaatacaa tggtaagtat aaatggggtg aagtaatggt
    11041 gaaataatcc ggaaactgcg ggttttaatc atactatcaa aacacttatc agctgttgac
    11101 gtataagttt cagcttttca agtattgaat ttgatgtcaa acagtttttc tcaatgtaga
    11161 attacttaat tttgcgactt attttaataa atatatatgt acaaagagtt taagggactt
    11221 aaccttccgg agattgacaa ggaaatactg caattttgga aatcagaaaa tatttttgaa
    11281 aaaagcattg ataccaaaga tgcttctaat ccttatgttt tttacgaagg gccgccgagt
    11341 gccaatggta tgccgggcat acaccacgtg atggcacgta ctgtaaagga tttgttctgt
    11401 cgttttcata ctatgctcgg tcagcgtgta gagcgaaaag gtggctggga tactcacggt
    11461 ctgcctatag aactaagtgt agaaaaagaa ctcagcatca ccaaagaaga tataggaaag
    11521 agcatatcag tagatgaata taaccacaaa tgccgcgaaa cagtgatgcg gtataagaaa
    11581 gaatgggata acataaccga acagatgggt tattgggtgg acttagacaa tccttacatc
    11641 acgtttgaga ataattatat agaatcagtt tggcatctat tacaattact ttataacaaa
    11701 ggcttgttgt ataaaggtta taccattcag ccttattcac cggcagccgg cacaggcctg
    11761 agtacacacg aactcaatca accgggttgt taccgtgagg tgaccgatac gtctgtagta
    11821 gcaatgttta aggtaatcaa ggatgaacga gcacagtttt tgtttgatga cgaagacgaa
    11881 gatgttagaa tcattgcctg gacaacaaca ccatggacac taccgtctaa taccgcattg
    11941 accgtagggt caaaaattga atatgttaag ataaagacca ttaaccctta taccaagcaa
    12001 gctttcagtg ctgtacttgc aaatgagtta gtgaatagtt ggttcggaga aaaaaaagca
    12061 gaaatattat caaaatcgga tgtcttttcc ggtgaaaaat tgaagggcat aagctatgag
    12121 caattgctcc cttttgcaca gcccgaggac ggagatgcct ttaaggtgat tacaggtgat
    12181 tttgtcacta cccaggatgg aacaggtatt gtgcatacag ctccttcatt tggtgctgac
    12241 gatatgcgcg tagcaagggc caatggtatt ggttcactca cacttgttga caaacagggt
    12301 aggtttactg cagaggcagg tgaattcgca agaaggtatg tcaaagacta caaggacgac
    12361 ccgaattatg taaatgtaga tatcgacatt gctgtaaagc tcaaacaaga aaacaaagcc
    12421 ttcaacgtcc aaaaattcgt acacaattat ccgcattgct ggcgtaccga taaaccggta
    12481 ctttactatc cgctcgatag ttggtttata aaaaccacag ctatgaaaga taggatggta
    12541 gagctcaaca agaccatcaa ctggaagcct gcctctaccg gtcaggggag gtttaccaac
    12601 tggctcgaga acctgcaaga ctggaactta tcgaggtcga ggttttgggg tattccgttg
    12661 cctgtgtggc gtactaaaga agccgatgat gaactctgca ttggctcggt agaagaatta
    12721 aagaagaaga ccgaacaagc caataaggca ctcaatttgc aacaaagcgt cccgactgac
    12781 ttgcacaggc cgtatattga cgaagttgta ctttattctg aaaaaaccgg aaagccg
//
LOCUS       DAOQQY010000052        12697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1702_length_12697_cov_5.792913, whole genome shotgun sequence.
ACCESSION   DAOQQY010000052 DAOQQY010000000
VERSION     DAOQQY010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12697
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1702_length_12697_cov_5.792913"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLE23_04295"
     CDS             <1..656
                     /locus_tag="PLE23_04295"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HMX82516.1"
                     /translation="EMDWNSAYGMLTHQGRMHPEIASFPNEQFYDGNLHSISSDTSYD
                     LLEKMRTKPGTGMDLYFKNRISFIDCPIATDEIHLKTNTVEAREVISVIDYLNQNMPP
                     GSKYDIGIITPFRAQIALIKNLLEVQKIDSSNITIDTVERYQGGARDIILISLCANKK
                     SSLRSITSVNKEGIDRKLNVALTRARQQVIIFGNKAILMADPTYARLMELYETKEYR"
     gene            complement(653..1285)
                     /gene="queE"
                     /locus_tag="PLE23_04300"
     CDS             complement(653..1285)
                     /gene="queE"
                     /locus_tag="PLE23_04300"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HMX82517.1"
                     /translation="MAYKIKEIYYTLQGEGAHSGRPAVFCRFSGCNLWTGREADRAKA
                     ICQFCDTDFWGMDGRNGGKYTAQDLVIQCKSLWPKNSDIPYYIVCTGGEPALQLDTEL
                     IEEFHKASCLVAVETNGTLLLPEGIDWVCMSPKADTEIVLQQVDEIKIVFPQPGINPL
                     NFEEIKASHYYIQPMDNTDQVSNTNECIKFCLENPKWNLSVQTHKYLKID"
     gene            1659..2678
                     /locus_tag="PLE23_04305"
     CDS             1659..2678
                     /locus_tag="PLE23_04305"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMX82518.1"
                     /translation="MYFKIGDKVLLKHLNEAGTVVSPPDIGMVQVELDNGETIPVFLE
                     DLIAYNPLSQANVPISAPKQKFTEDSRIIEFPVSKHSKTGLWTAFIPKFNQYDEILYY
                     TAYLLNDSKNDIVFEIEISTKAFKLRHDNILKQVQAFLIGRIEPEMIESQLTVVVSLS
                     DYYTSGPENKRTQSLRITPKKFFSRDDLVPFFDQMGYLFEFIPDVQKVEDGESLKDFT
                     EKILKSKPVQVKNKPKYVSTTDPLRKSAFEPQIDLHAEKLLRPGQKLEEHMIMQLQMR
                     VFAEYMEEAIRLGMDKVYIIHGRGNGKLKSAIARELRNNPYVIDFQNEYHNKYGFGAT
                     EVWLR"
     gene            complement(2679..5231)
                     /locus_tag="PLE23_04310"
     CDS             complement(2679..5231)
                     /locus_tag="PLE23_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82519.1"
                     /translation="MKAQLLCLVAILVLSIKGELSAQFQDCNTAQVLCTKDPVVVNSV
                     TGPGNFEDVMNNSCFEDQEHQSHWFTFYATKTGTFEFSIKPDGYLADYDFAMWEGGCP
                     GTPGSSEVACNWLGAVVVPPYFATGVATDPAASFGEGSSLEFINTIQVNAGQVYYLLV
                     DNITANGKGFTIYFNGTAEIGNPELSFTAGVFCNQSSQDLKTIKVEGLDSVPGTIEYF
                     NKYSDAINGTNALANSVVTSNGNYYVTKTTPHGCKATQTISVTLENPNVLIGDVYTCG
                     NPSTFDFNDLKKKEISGLDLADISFTYFLNQQDMLNNTNPIGNIVTVSQVIWAKAITK
                     NGCVDLIPFNVNLEKPVISLSGTEEICPGKSVFLPINYNGRWPINVTVAINGGLNIKD
                     FLIQGEPTVVQPQISTVYTITECIDTFGCSATVQGSYKVIVHKSPEIVNVVTDCSIYN
                     GKPAFVVTVTGGDTASYQIDGIPGKFSDNIFVSDPLIEGNMYSFQLYDAYKCAVVQWT
                     GEPTCDCDPNFAVSIFENTPIQCFGDTNGELLANVSGGFPPFSYKWSEGSTTAKLSNL
                     SVGNYTVTVTDGNACTAVGTYTLTSPPQITLQYEILNPLCENEAGGSINITNIKGGTA
                     PYIISLDGKIVPGVPAVFDQLNAGSYKINVNDVHNCKLDIPVEIKAGLPFNLELGPDI
                     SLAEGETAKIALTGDLAEIVNIEWAGSYPYNCGNCTEFVYTPTGSGFFSFVASNANGC
                     QATDTVNIALHLKSLSDKVFLPNTFSPDGDGVNDVFRPYYGNIAVAKSTLSVYNRWGA
                     QLYFEEFTDEANGWNGKFNGKILNPDVYVYVLTVELANGETSVFKGDITVFK"
     gene            complement(5306..10534)
                     /locus_tag="PLE23_04315"
     CDS             complement(5306..10534)
                     /locus_tag="PLE23_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82520.1"
                     /translation="MTRVLNYKSILSVVFLIYFLLGINKSIAQPCQCINCPGTIPKAG
                     NQDFSTTEFLYFVNGAESNSLEDLFQGVCAVNVVFKADNVFQVEMYLISPGGDTVRLV
                     GPDAGSGVGVTALTTWSVAFMPSSIAVFPDPGFSPVWTNYDPWETGANYTGTYYPNLG
                     KLEDFDSGPVNGPWRLVVKNYAPLNKGEVIDFSIVFCDETGIECGCLAYAGIYPSFQI
                     IEACQGSDKLKLNLNPNYNGYEPDPKVFNYTYTIASPKNVILEFTEAPDLSGYAVGNY
                     KVCGLSYLQADSALVIKDAGITKLDTIYKEISSPDPDYCADLSKNCIFININPPPPPV
                     NIEQFLCQDSCITFHGKTYCMPGSYTFTELDSLGCSINYNLKLNPATTKYYSYTDTIC
                     VGEFVIFGVDFYNKTGIYTKTFKSFTGCDSIVTLNLKVMNFDIKVKKPDTLNCVHEQV
                     LLDGSDVNENNPSFSYLWTASGGGEVIGPGNMPDLWVSKPGTYKLTGTFTLSSGKTCT
                     DFVNVTVAENIIQPELTTVPDQFLCLGDELDLSKLNIKEIHGMPGTLSYHSANPPDAT
                     NQISPLIQPTVSGVYYAHFVVGKCDAVKQINVNVNKKPKAEVKPLLNVCNSAMMGENT
                     LVNFDTLIVSGDNKGYWTDKDFSGATGTFPLLDFTGVKPGTYEFVYTTQSAIPPCTDT
                     FYIVNVIVEDCECPSLATLSPGDMCQNSAKSDLNALLITTESGNWSLESAPPGSTASI
                     NGSIFDGTGSTPGNYSIKFTLSQIPPQGCQESVVLNLKIVAPPELQMMSDITVCNSDD
                     MGQGTTVDMLQTIVAGNKTGIWKDIDNSGAVGIFPVLDFKNIAPGIYRFRYETNSAEF
                     PCPEVYDTVNVTVKDCNCPTVSLLSTVLCNIKDTLLLNDLVITGQAGTWQLLTSPSGA
                     NPAKIIADKFIFDNSNAGKYELKFVLSNPVAGCQSDFITTVELLQAPFTELDTVKNIC
                     NSNQNGHISLLDFSTLLKAGDLGGSWQQVVPSGATGTLPLLDFNGVAPGSYYFKYITN
                     SAVSPCKETEYLIRIIVEDCSCPSVTTVNPGKFCNNNLSINLDNYKVTTEAGSWAIVM
                     TPPGTNPATVVLNTFSGTNADPGIYTIQFNLQANPPFGCPTFSIQTIEVVADLTADII
                     QMVSVCNDGSAGNVSTVNFSSLITKGSQNGVWTDVDNSGATGTFPLLDFSGVTPGVYT
                     FSYSLKANSPCQDKQYLVMVEVKDCKCPPVLQKDIPTVLCNDGISIDLNNYFSANVVS
                     ALISIPSGQSATILQNNILQLQDIMPGEYTILAALASVPAGCKKDTLLTLQIVDSKNA
                     GTPIKDFVFCEAEDTLLNLNQLLTGADFGGQWLLVPASAALNTLTGTLDINLLSAGSY
                     SLKYIQNNAPPCKVEEALINIVINKVPQADAGEDQQLGCSKAYAVLGGTGNPGLTNVS
                     FEWSNNVPDANAGNPEVTLAGTYFLTVTDLTTGCSSEDAVTITQGTDSDIEAKINLKQ
                     PSCSGNSTGSISIEVIKGNPPYLYSINQLPFQPENIFDNLVSGLYTISIEDAAGCTKD
                     TTVEIFSALTGGISLGKDTIINFGDSVFIEPNLKFPVSELASVKWSSLGYISCDTCLN
                     TYLKPFIPTKYFLEVQTKAGCYYKADIIVTVNRDVKYFVPNIFSPNGDGVNDWVEISL
                     GPDIVSMQEFSLFDRWGEQIYKLNNVTNDGIPIKSWDGLFKGSKVNSGVFVYMMKLVL
                     VDGTIEFVTGELTLVK"
     gene            complement(10717..>12697)
                     /locus_tag="PLE23_04320"
     CDS             complement(10717..>12697)
                     /locus_tag="PLE23_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX82521.1"
                     /translation="SDTIEIAQYIQSITVPVVDFDVVMNGNVAVCTNISLYGATYLWD
                     FGDGTTSNQENPVHTYGQAGNYTIKLTVTNDCGSNSVTKNVTAVFKPTASFTSDKVQG
                     CATLTVNFQNTSSADATFFNWTFIGGNPATSTLKNPVVTYDTPGSYTVSLVAGNSAGT
                     DTSMIVAYITVAGAPIPSFTYTNNSDGKVHFTNTSQNGISYLWDFGDGATSTEKDPIH
                     KFETSGVKSVTLTVTNDCGQTSTTIVVVVIFPPSAEFSSDKNTGCGAQTITFEDLSTE
                     GAVQWLWYFEGGSPSTSTDKNPVVQYNTPGSYDVTLIASNSIGSDTIVKPGFVLISSA
                     PPVADFTYTSTGLTHNFTNQSTDAYSYLWNFGDGGQSTEPDPVHNYLDGGVYTIQLIA
                     FNGCGSDTITENITVVGSVPEGSFSSDITSGCAPLIVKFTDKTLGGATAWQWSFPGGE
                     PATSTEQNPTVIYNTAGVYDVTLIVSNLFGSDTIENFSYIVVNSVPLAKFSYTIQSGV
                     VTFINQTTGGTNYLWNFGDGTSSTEQNPTHAYANPGQFTVTLTVTNECGSYTFTQIID
                     PKVGVREIDFIKNIKLFPNPNAGHLTLEINSASGENLMLKVVDIMGKMVYTKELKLTN
                     GLSKTELNLQNLASGTYMVILESDKGILVRKMMIQ"
BASE COUNT         3855 a         2627 c         2430 g         3785 t
ORIGIN      
        1 aagagatgga ttggaattca gcttatggaa tgcttacaca tcaaggtcgg atgcatcccg
       61 aaattgcaag cttcccgaat gagcagtttt atgacggtaa tctacacagt atttcatcag
      121 atacatcgta tgatctgtta gaaaagatgc gtactaagcc aggtacaggc atggatctat
      181 attttaagaa tcgaattagt ttcattgatt gcccgatcgc taccgatgaa atacacttaa
      241 aaactaatac cgttgaagct cgtgaggtga taagcgtaat tgactatctt aatcaaaata
      301 tgccgcctgg aagcaaatat gatatcggga tcataacacc attcagagca cagattgctt
      361 tgataaaaaa tttgttggaa gtacagaaaa ttgattctag taatataacg attgatactg
      421 tcgaacgata ccaaggtggg gcgagggaca tcatcctcat ttcactttgt gccaacaaaa
      481 aatcttcctt aagaagtatc acgtcagtca acaaagaagg gattgaccga aaactgaacg
      541 ttgcccttac ccgagcacgt caacaagtaa tcatttttgg gaataaagcc attctgatgg
      601 ctgatcctac ttatgccaga ttaatggaat tgtatgaaac aaaagaatac cgttagtcta
      661 tttttagata cttgtgtgtc tgtacgctaa ggttccattt tggattctcc aggcaaaact
      721 ttatacattc attagtattt gacacttgat cagtattatc cataggctgg atatagtagt
      781 gagatgcttt gatttcttca aaattcaggg gattaatacc tggttgcgga aatacgattt
      841 ttatttcatc aacttgttgc agaacaattt cagtatccgc tttcgggctc atacaaaccc
      901 agtcaatacc ttcaggcagt aacagtgtac cattcgtttc gacagctacc aggcaagatg
      961 ctttatgaaa ttcttcgata agctctgtat cgagttgtag cgccggctca ccaccggtac
     1021 atacaatgta gtatggtata tcagaatttt ttggccacag cgatttacat tgtattacca
     1081 agtcctgtgc cgtatatttc ccgccatttc taccgtccat cccccagaaa tcggtatcac
     1141 aaaattgaca aattgctttt gctctgtcag cttctctgcc agtccaaaga ttacaaccac
     1201 tgaatctgca aaaaacagcc gggcgaccgc tatgtgcacc ttcaccttgt aatgtgtagt
     1261 aaatttcttt aatcttatat gccaagacga tgattattgt tatttcaaaa ctcaacgatt
     1321 caggactgtg actgtgcctg acaaaatagc aatacaaagg taagcaagta aatttatttc
     1381 tttgatatta aaatccaatg cccggatagg tatttagtca cattctgtat ctatttttac
     1441 attcagatat taacccagat aacgcattag gctctttatt gcaaaaactt attacttcaa
     1501 atccattgca aaagttcaaa aaccatagtt cactatgctt ctaactcttt tttatggatt
     1561 ttttgtacga atcattttcg tttaaattcc taatacgtta actggggtta agtaaacatt
     1621 cctgtattat ttttgttaaa tagtgcgaaa aagtaattat gtatttcaaa attggagata
     1681 aggtattact caaacatctc aacgaagcag gtactgttgt ttcgcctcct gatatcggca
     1741 tggtacaggt cgaactagat aatggcgaga ccataccggt atttttggaa gacttaattg
     1801 catataaccc tctatcgcaa gcaaatgtac caatatcagc tccaaaacag aaatttacag
     1861 aagattcaag aatcatcgaa tttccggttt caaaacatag caaaaccggt ttgtggaccg
     1921 catttatacc aaagtttaac cagtatgacg aaattctgta ttatacagca tatttactga
     1981 atgattcaaa gaacgatatt gtttttgaga ttgagataag cacaaaagca tttaaactta
     2041 ggcacgataa tattttgaag caagtgcaag cttttctgat aggacgtata gagcctgaaa
     2101 tgattgaatc acagctcaca gttgtagttt cattatcaga ctattacaca tcaggccctg
     2161 aaaataaacg aacacaatcc ttgagaatta ccccaaaaaa attcttctca cgtgacgatc
     2221 tcgtcccctt ctttgaccaa atgggttatt tgtttgaatt catccctgat gtacaaaagg
     2281 tcgaagatgg tgaaagttta aaggatttta cagagaaaat attaaaaagc aaaccagtgc
     2341 aggttaaaaa taaaccaaag tatgtatcga ctactgaccc actcaggaaa tccgcttttg
     2401 aaccacaaat agatctccat gcagaaaaat tgttgcgacc cggtcaaaaa ttggaagaac
     2461 acatgattat gcagctccag atgcgtgtct ttgctgaata tatggaagaa gctatacgct
     2521 tgggtatgga caaagtatat atcatccatg gcagaggaaa cggaaagcta aaatcagcca
     2581 ttgcaagaga actacgcaac aacccctatg ttattgattt ccagaatgaa tatcacaaca
     2641 aatatggttt tggcgcaaca gaagtgtggc tgagataatc acttgaaaac cgtaatatca
     2701 cctttaaaaa cagatgtttc gccattggca agttctaccg ttagtacata aacatacaca
     2761 tcaggattga gaatctttcc attgaactta ccattccaac cattggcttc gtcagtaaac
     2821 tcttcaaaat atagttgtgc tccccatctg ttataaacac tcagtgtaga tttggctacc
     2881 gcaatattac cataataagg tctgaagaca tcattgacac catctccatc cggactgaat
     2941 gtattcggta ggaacacctt atcgctcaga cttttaagat gcaaagcaat gttaacggta
     3001 tctgttgctt gacaaccatt ggcattactt gcgacaaaac taaagaaacc actaccggtt
     3061 ggtgtatata caaactctgt acaatttcca caattatatg ggtaactacc agcccactca
     3121 atattaacta tttctgccaa atcacctgta agtgctatct tggcggtttc gccttcagct
     3181 agactgatat caggcccaag ttcaagatta aagggcaatc cggcctttat ctcaacaggt
     3241 atatccagct tgcagttatg tacatcattt acatttatct tatagctgcc cgcattcagc
     3301 tgatcgaata cggccggtac tccaggaact atcttaccat caagcgagat gatatatggt
     3361 gccgttccgc ctttaatatt ggtgatgttg attgaaccac cggcttcatt ctcacacaat
     3421 ggattcaaaa tttcgtattg gagtgtaatc tgaggtggtg aagtaagtgt gtaagtacca
     3481 acggcagtgc aggcattgcc atcagtaacc gttactgtat aattacctac cgaaagattg
     3541 gaaagttttg cagttgtact accttcgctc catttgtatg aaaaaggtgg gaaaccccca
     3601 cttacattcg ccaacaattc cccgtttgtg tcaccaaaac attggattgg cgtattctca
     3661 aatattgaca cagcaaaatt cgggtcacaa tcgcaggtgg gctcacctgt ccattggaca
     3721 actgcacact tgtaggcatc gtaaagttgg aaagaataca tattcccttc tatcaatggg
     3781 tcagaaacaa atatgttatc ggagaattta ccgggtatgc catcaatctg atagcttgca
     3841 gtatctccac ctgtcactgt gacgacaaaa gccggtttgc cattatatat ggagcaatct
     3901 gtcacaacat tcacgatttc cggactttta tgaacgatta ccttgtagct accttggact
     3961 gtagccgaac aaccaaaagt atcgatacat tccgtgatgg tataaacagt ggaaatctgt
     4021 ggctgaacaa ccgtaggctc gccctgtatc agaaaatctt tgatattgag ccctccatta
     4081 atggctactg tcacatttat tggccagcga ccgttatagt tgattggcag gaatacagat
     4141 ttgcccggac aaatttcttc ggttccgctt aatgaaatga ccggtttttc aagatttaca
     4201 ttaaaaggta tcaaatcaac gcatccgttc tttgttatgg ctttagccca aatcacctga
     4261 ctgacagtga cgatattgcc aattggattg gtattattaa gcatatcttg ctggttgaga
     4321 aaataggtaa aacttatatc agccagatca agcccggaaa tctctttttt ctttaggtca
     4381 ttaaaatcga aggtagaagg attgccgcag gtatatacat caccaatcaa tacattcgga
     4441 ttttcaagtg ttacactgat ggtttgtgtt gccttacagc catgaggtgt agttttggtg
     4501 acgtaatagt tgccgttgga agtgacaaca gaattagcaa gcgcattcgt cccgtttata
     4561 gcgtcagaat acttattaaa atactcaatc gtaccaggta cagaatccag gccttcaact
     4621 ttaattgttt taagatcctg ggaagattgg ttacagaaca cgccagccgt aaatgataat
     4681 tcaggattgc ctatttcggc tgttccatta aaatatatag taaaaccttt accattggct
     4741 gtaatgttgt ccaccaatag gtaatacacc tgccctgcat tcacttgaat ggtattgata
     4801 aattccagac tactgccttc accaaaagat gcagcaggat cagtagccac acctgtagca
     4861 aaatatggag gtaccactac tgcgcctagc cagttacagg ccacctcact actacccggt
     4921 gtacccggac aaccaccttc ccacatagca aaatcgtaat ctgcaagata tccgtcaggt
     4981 ttgatagaaa attcaaatgt acctgttttt gtagcgtaaa atgtaaacca gtgactttga
     5041 tgctcttggt cttcgaagca tgaattgttc attacatctt caaaattacc cgggcctgtc
     5101 accgaattga ctacaaccgg gtcttttgtg catagcactt gtgctgtatt acaatcctga
     5161 aactgtgctg ataattctcc tttgatactg agtaccaaaa ttgcaacaag acaaagaagt
     5221 tgggctttca ttttgtagga ttataattaa atatgcttta acgcaaaaaa gtaaacaaag
     5281 ttagtaatta ggttcttaat tttccttatt taacaagtgt cagttcacct gttacaaact
     5341 caattgtacc atccaccaac accagtttca tcatataaac aaatacaccg gaattcactt
     5401 tgctaccctt aaacaaacca tcccaactct taatcggaat accatcattg gtcacattgt
     5461 tcaatttgta aatttgctcg ccccagcgat cgaaaaggct gaattcttgc atacttacaa
     5521 tatcaggccc taaacttatc tccacccaat cattcacacc atcaccgttc ggactaaaaa
     5581 tattaggcac aaaatattta acatcccgat tgactgtaac aattatatct gccttgtaat
     5641 aacaaccagc ctttgtctgt acttcaagaa aatatttggt tggaataaat ggtttcaaat
     5701 aagtattgag gcatgtatcg caactgatgt aacctaaact tgaccatttc actgatgcca
     5761 gttcgcttac cggaaacttc agattgggtt caatgaatac actgtctccg aagttgataa
     5821 ttgtatcttt ccctagcgaa ataccaccgg taagtgccga aaaaatctca acagtagtat
     5881 ccttagtgca acctgctgcg tcttctattg aaatggtgta taaacccgat acaagattgt
     5941 cgaaaatatt ttccggctgg aaaggaagct gattgatgct gtataggtac ggaggattcc
     6001 cttttatgac ttcaatagaa atgctacctg tgctattacc tgagcatgaa ggttgtttaa
     6061 gattaatctt cgcttctatg tctgaatctg ttccttgggt aatagttaca gcatcttcac
     6121 ttgagcagcc ggttgtcagg tctgtaacag taaggaagta ggttccggca agtgttactt
     6181 ccggattgcc tgcatttgca tcaggaacat tgtttgacca ttcaaaggag acattagtca
     6241 gtcccggatt gccggttccg ccaagtactg cataggcttt gctacatcca agttgttggt
     6301 cctcacctgc atccgcctga gggactttat ttatgacaat atttattaag gcttcttcaa
     6361 ccttgcaagg tggagcatta ttctgtatat attttaagct atagcttccg gcagacaata
     6421 agttgatatc aagagtgcct gtcaaagtat taagtgctgc agaagcaggt acgagcaacc
     6481 attgcccacc aaaatcagca cctgtcagta attggttaag gttcaacaag gtatcttctg
     6541 cttcgcaaaa aacaaaatcc ttgattggtg tacctgcatt ttttgagtca acgatctgga
     6601 gtgtcaataa tgtatctttt ttacaacccg caggtacaga agcaagagca gctaaaattg
     6661 tatattcgcc cggcatgata tcctgtaatt gtaaaatgtt attttgtaaa attgttgccg
     6721 actgcccgct cggaatactt atgagtgccg aaacaacgtt ggctgaaaaa taattattga
     6781 gatcaatact gataccatca ttgcataata ccgtcggaat atccttctgc aatactggtg
     6841 gacatttgca gtctttcacc tctaccatca caaggtattg tttatcctgg caaggagaat
     6901 tagctttcaa tgaataacta aatgtatata caccgggtgt tacacctgaa aaatccaaaa
     6961 gcggaaaagt gcctgtcgcg cccgaattat caacatctgt ccagactcca ttttgtgacc
     7021 cttttgtgat gagtgaagaa aaattaacgg tagaaacatt accggctgat ccatcattgc
     7081 aaacagaaac catttgtata atatcagcag taaggtcggc caccacctca attgtttgta
     7141 ttgaaaatgt gggacaacca aaaggtggat ttgcctgtaa gttgaattga atagtataga
     7201 ttcccgggtc tgcattagtg ccggaaaatg tgttaagtac tactgtagca ggattagtgc
     7261 ctggtggagt cattacaatt gcccatgacc cggcttcggt ggtcactttg taattatcaa
     7321 ggttgatact tagattatta ttacaaaact tacctgggtt tacagtggtc acactggggc
     7381 aactacaatc ctcaacgata attcttataa ggtactcagt ctccttgcat ggagataccg
     7441 cagagtttgt tatgtattta aaataataac tgcccggtgc cacgccatta aaatcaagca
     7501 atggtagagt gcctgttgcc ccggagggta ccacttgttg ccacgagcca cctaggtctc
     7561 ctgcttttag aagtgtggaa aaatcaagca gactaatgtg accattctgg ttgctgttgc
     7621 aaatattttt cactgtgtca agctcagtaa aaggcgcttg taacaattca actgtggtaa
     7681 tgaaatcaga ttggcaacct gcgacaggat tactcaaaac aaatttaagc tcatatttac
     7741 cggcattgct attatcaaaa ataaatttat ctgcaataat ttttgccgga tttgctcctg
     7801 acggcgatgt caataattgc caagtacctg cttgacccgt tattaccaga tcatttaaca
     7861 gaagcgtatc ttttatattg cacaacactg tactgagcaa actcactgtc ggacaattac
     7921 agtccttgac ggtcacatta acagtatcat atacttcagg acaaggaaat tctgcagaat
     7981 ttgtttcgta cctgaaccta tatatacccg gagcaatatt cttaaaatca agcaccggaa
     8041 aaatacctac tgcaccagaa ttgtcgatat ctttccaaat tccggtttta ttacctgcaa
     8101 cgattgtctg caacatatca acagttgtgc cctgccccat atcatccgaa ttacacacgg
     8161 taatatcaga catcatttga agttccggtg gtgcaacaat tttcagattc aatacaacgg
     8221 actcttgaca accttgtggt ggaatctgac tgagtgtaaa ttttatacta taattacctg
     8281 gtgtggaacc tgttccgtca aatattgatc cgtttataga tgccgtagag cccggaggtg
     8341 cactctcaag actccaatta ccggattctg tggttatcaa caatgcattc agatcagatt
     8401 ttgctgagtt ctggcacata tcacccggtg aaagtgttgc cagacttggg cattcgcagt
     8461 cttctactat cacattaacg atgtagaatg tatctgtaca aggaggtatt gctgattggg
     8521 ttgtataaac aaattcgtaa gtgccaggct tcacacctgt aaaatcaaga agaggaaatg
     8581 tgccggttgc acctgaaaag tctttgtctg tccagtatcc tttgttatcg ccggaaacaa
     8641 taagcgtatc aaaattaacc agcgtatttt cccccatcat cgcactattg cagacattga
     8701 gtaatggttt tacctcagct tttggttttt tattgacatt tacatttatc tgcttaactg
     8761 catcacattt tcctacaaca aaatgtgcat aatatacgcc tgatacagtt ggctgtatca
     8821 aaggagatat ttgattggtt gcatccggag gattggcaga atgataacta agcgtacccg
     8881 gcataccgtg tatttcctta atatttaact tagaaagatc aagttcatca ccaagacaaa
     8941 gaaactgatc gggtacggtt gttaattcgg gttgaattat attttcagca acagttacat
     9001 ttacaaaatc agtacaagtt tttccggatg aaagtgtaaa agtgccagtc agtttatacg
     9061 ttcccggctt tgaaacccac aaatcgggca tattgccagg tccaataact tcacctcctc
     9121 cgcttgcagt ccataagtaa ctaaatgaag gattattctc attaacatca gacccatcga
     9181 gtagtacttg ttcatgaaca caattgagtg tatccggttt ttttacttta atatcaaaat
     9241 tcatcacctt caggttcaat gtaacgatgc tgtcacaacc tgtaaaggat ttgaaggtct
     9301 tggtataaat accggtctta ttataaaaat cgacaccaaa tattacaaat tcaccaacac
     9361 aaatggtatc ggtatagcta taatattttg tagtagcggg gttaagcttc agattatagt
     9421 tgatagaaca accgagtgaa tcgagctcag taaatgtata gctacccggc atgcagtagg
     9481 tcttaccatg aaaagtgata cacgaatcct gacacaagaa ctgttcaata ttgacaggtg
     9541 gaggtggtgg gttgatatta ataaatatac aattcttact caagtcagca caataatccg
     9601 gatctggact gcttatttcc ttataaatag tatccagttt tgttatgccg gcatctttaa
     9661 taacaagcgc tgaatcagcc tgcaagtagg ataagccaca caccttgtaa ttacctaccg
     9721 catatcctga aaggtcgggt gcctctgtaa actccagaat tacatttttg ggtgaagcga
     9781 tggtgtatgt ataattgaat actttagggt caggttcgta accgttatag tttggattaa
     9841 gattgagttt tagtttatct gaaccctggc aagcttcgat aatctgaaaa gatggataaa
     9901 tgcccgcgta agcaagacat ccacattcga taccggtttc gtcacaaaat acaatagaga
     9961 aatcaatcac ttctccctta ttgagcggag cgtaattttt tacaaccagc cgccatggtc
    10021 cgttgacagg tccgctgtca aagtcttcga gtttacctag gtttggataa tatgttcctg
    10081 tataattggc gcctgtctcc catgggtcgt aattggtcca gaccggacta aaacccgggt
    10141 caggaaaaac ggcaatcgaa ctcggcataa aagctacact ccacgtagta agagctgtca
    10201 cacctacacc tgagcctgca tccgggccga ctagccgaac tgtatcacct ccgggtgaaa
    10261 tgagatacat ctcaacctga aaaacattat ctgctttgaa aactacattt acagcgcaaa
    10321 ctccctgaaa tagatcttca agcgaattgg actctgcgcc atttacaaag tataaaaact
    10381 ctgttgttga aaaatcctga ttccctgctt tgggaatggt acccggacaa ttgatacact
    10441 gacacggttg tgcaattgat ttattaatac ctaataaaaa gtatatcaga aaaacaactg
    10501 acagaatact cttatagtta aggactcgtg tcatattata cggaattccg ggattgataa
    10561 ttaaaaacag tttgtttagg caatttaata ttttttgtac taaatcgcaa gcaaaattaa
    10621 aacaattttt gtcagttact cgatgtatca cttacaaatg attccggcca ggtaaatatt
    10681 gttgcccggc cggaaaaatc ttaagttgta tttttgttat tgtatcatca tctttcttac
    10741 caatatccct ttgtccgatt caagtataac catgtaggtg ccggatgcca ggttttggag
    10801 attaagttca gttttgctca gcccgtttgt gagtttgagt tccttagtat aaaccatttt
    10861 acccataata tcaactactt taagcatcag attttctccg gaagccgaat ttatttctaa
    10921 tgtcagatga ccggcatttg ggttcgggaa gagtttgata tttttgataa aatcaatctc
    10981 tctgacaccc actttcggat cgataatctg ggtaaaagta taagatccgc attcattcgt
    11041 tacggtcaat gttactgtaa actgtcccgg attggcataa gcgtgtgtcg gattctgttc
    11101 ggtagagctt gttccgtcac cgaagttcca cagataatta gtaccacctg ttgtctggtt
    11161 gataaatgtt acaacgcctg attgaatagt ataactaaac ttagccaaag gcacactgtt
    11221 tactactatg taagaaaaat tctctattgt gtcagaacca aacaagttgc taactatcag
    11281 tgtcacatca tagactccgg ctgtattgta tatgactgtt gggttttgct cagttgaagt
    11341 agccggctcg ccaccaggga atgaccattg ccatgctgtg gcaccaccca aggttttatc
    11401 tgtaaatttt acgatcaaag gtgcacaacc ggatgttata tctgaactaa aactaccttc
    11461 aggtaccgaa ccaacaactg ttatattctc agtaatggta tcactaccac aaccattgaa
    11521 agcaatcaac tggattgtat aaacgccacc gtcgaggtaa ttatgaaccg gatcaggctc
    11581 tgtactttgg ccaccgtcgc cgaagttcca caaataactg taggcgtctg ttgattgatt
    11641 ggtaaaatta tgtgtcaatc ctgtacttgt ataggtaaaa tcagctactg gtggtgctga
    11701 acttatcaat acaaatccag gttttacgat ggtgtcactg ccaatactgt tggatgctat
    11761 caatgtcaca tcatacgagc ccggtgtgtt atattggacg accggatttt tatcggtaga
    11821 agtagaaggt gagcctcctt caaaatacca taaccactgt actgcacctt ctgttgaaag
    11881 gtcttcgaaa gttatggttt gtgcaccaca gcctgtgttt ttgtcagacg aaaattccgc
    11941 cgaaggaggg aaaattacaa ccaccacaat ggtagtactt gtctgtccgc aatcattggt
    12001 aacagttagt gtcacactct taacaccgct ggtttcgaac ttgtggatcg gatctttttc
    12061 agtagaggta gctccatcac cgaaatccca tagatacgaa ataccgttct ggcttgtatt
    12121 cgtaaaatgt actttaccat cagaattatt cgtataagtg aatgaaggta taggcgcccc
    12181 ggcaacagtt atataagcta ctatcatcga agtgtcggtt ccggctgaat taccagctac
    12241 caatgagacc gtatagctac ccggagtatc ataagttaca accggatttt taagtgtgct
    12301 cgtcgccgga ttgccaccga taaaggtcca gttgaaaaac gtagcatcag cacttgatgt
    12361 gttttgaaag ttaaccgtca aggtggcaca gccttgtact ttatcagatg taaaggaagc
    12421 cgtaggtttg aatactgcgg tgacattctt ggttacagag ttgctgccac agtcattggt
    12481 tacagtcagt ttgatggtat aattacctgc ctggccataa gtatgaaccg gattttcctg
    12541 attactggta gtgccatcgc caaaatccca taaatatgta gctccataaa gtgatatatt
    12601 ggtacacacc gctacatttc cattcatcac aacatcgaaa tcaacaacag gtacggttat
    12661 ggactgaatg tactgggcta tttcgattgt atcactt
//
LOCUS       DAOQQY010000053        12597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1728_length_12597_cov_6.390129, whole genome shotgun sequence.
ACCESSION   DAOQQY010000053 DAOQQY010000000
VERSION     DAOQQY010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12597
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_12597_cov_6.390129"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            35..439
                     /gene="mce"
                     /locus_tag="PLE23_04325"
     CDS             35..439
                     /gene="mce"
                     /locus_tag="PLE23_04325"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HMX82522.1"
                     /translation="MEKLEHIGIAVKDLAVSNQLFEKILGAPHYKIEEVTSEYVNTSF
                     FKIGETKIELLEATHPDSAIAKFIEKKGEGIHHLAFEVSDIHEAMLRMEEQGFTLLNK
                     TPKRGADNKLICFLHPKTTNGVLIELCQSIEE"
     gene            441..3641
                     /locus_tag="PLE23_04330"
     CDS             441..3641
                     /locus_tag="PLE23_04330"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HMX82523.1"
                     /translation="MNTHQSAYEAAFAKLNEGQRQAVLATEGPVMVLAGPGTGKTQVL
                     ATRIGYILDNTDTNPQNILCLTFTDAGVVAMRERLVKFIGKEAYKVNIHTFHSFCNMV
                     IQANTEYFTQNFQLNVADDLQKKEIVRKMIDLLPVDDVNKRLKNAFYLDENKYMSLFS
                     EMKRENISTEQIEADGQFVMQRVEDFEDMVYLRKSGENNKGDKKLKDIAKIREGVGKL
                     VAASRQLETYNALLAEDGLYDYDDMINWVVNAFERDENLLLDYQEQYLYIMVDEFQDT
                     NGIQIKLLNQLCSFWGDSPNVFVVGDDDQAIYRFQGANVDNLSSFYEKYKPAIVCLTK
                     NYRSKPDIITTAKNLITNNKSRLGSAIPGVVKDILPGKECEDNGSVAAFQYANNEQED
                     TAIICWIENHIKAGVKPKQLAVVSRNHDGLRELVNILQKKQIPVFVKTKVNALHIPVV
                     RNLVTILRFLDSCLRDPYDAHICLAEIIQYPNQGIQSFDVLKLIRYVDEINFNKENKE
                     KIWLAEIIGSEKHLQAAGVSDADVILSLSRKIENWLAAVHTETIQVLFEKILSESGIL
                     EFVLNSANKINLLEAIHVLFNFIKEKTTVDDSFNLHSLVVLIKKMEELKIDLPFIKIT
                     GNSEGVQMMTAHATKGLEYPYVWIKGADAAKWEVKRGSSGSFMSGLFKAILRIPDVAR
                     KKDAEEWSKDIQLEDERRLFFVATTRAEEELIMSYSPKTSDKKETPSRFLIESMQTDE
                     IPTAEVNQTALVEHLALKIRKSELDQENVEREYLDRILKDFKLSSTALNNYLKCPLRF
                     YYSNIMRIPQARSAGMGFGNVVHKVLERLWKYQKEAGGWDAVHDLDTRIVQYIEEAMK
                     QFQSHFNPTELKNNFEEGKKKLPLFIKSQLAAWRNIPDFKTEYKIQTILDGKIPLTGN
                     LDRLDILKDDIHVTDYKTGSLRPEQLKSGLADDKGIGGDYWRQMVFYKILVDLDNSFS
                     RQMTSGTMQYVTDEKNGVFSSQKFTITPEEIQMVSEQIEDTYAKILRHDFYKGCGKQD
                     CHYCHFVNDIKSLSLADESDAEYSNGDDFASPQSDE"
     gene            complement(3670..4194)
                     /locus_tag="PLE23_04335"
     CDS             complement(3670..4194)
                     /locus_tag="PLE23_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HMX82524.1"
                     /translation="MISNTMEAALNKQIELEAYASFLYLSMASWCDKEGLNGCAAFMH
                     RQSDEERQHMLKIFHYISEVDGYALTPAIAQPPHQFESVQKLFEQVYEHEKGVTRAIN
                     HLVELSNKEDDYTTLNFLQWYITEQREEEALMRSILDKIKLIGSGPMSLYYIDQEISS
                     INAAQLKADNAEGA"
     gene            complement(4219..5406)
                     /locus_tag="PLE23_04340"
     CDS             complement(4219..5406)
                     /locus_tag="PLE23_04340"
                     /inference="COORDINATES: protein motif:HMM:NF040152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5706 domain-containing protein"
                     /protein_id="HMX82525.1"
                     /translation="MYQPLEPVLRESENFIREYFQKNMPAQYSFHNFDHTAQVVHSAY
                     LIGEREGLSTQDMHLLKIAAWFHDTGYTEGGMGHEARSVLVAENFLKGKLSEEHIMVI
                     KDIIMATKMPQSPKTLNERIICDADLAHLGNEHFWERNDLLKAEMEANGIKMNKNEWL
                     QFEINFIANHHYHTDSGRYLFENNKQLFLKQLYYELNKRKEKNSTEQPNPVENFDFEQ
                     KKLSRGSDTLFRTSYNHHLNLNTLADSKAHIMMGVNSIIFSVVGSNLIPKWQTDYRLI
                     IPTIFLLGACLLSVFYAILTIKPKLIKGNVSLDEVKEKTADLLFFGNYTGMKLPDYQW
                     AMRQLIQDQQYIYDSMSKSIYFLGQVLNKKYRYLTYCYNVFLYGLLISVVLFSVCLLL
                     IPK"
     gene            complement(5519..5857)
                     /locus_tag="PLE23_04345"
     CDS             complement(5519..5857)
                     /locus_tag="PLE23_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMX82526.1"
                     /translation="MEGSLLFVADSAKEKIKQIRESGHITDDYFVRVSVISGGCSGLS
                     YKMDFDNEPQPNDQIFEDNGVKIVSDLKSFLYLFNSTLDFSGGLEGKGFFFRNPNASR
                     ECGCGESFAV"
     gene            complement(5862..6254)
                     /gene="iscU"
                     /locus_tag="PLE23_04350"
     CDS             complement(5862..6254)
                     /gene="iscU"
                     /locus_tag="PLE23_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HMX82527.1"
                     /translation="MAYSEKVLDHFKNPKNVGTLDKNKKNVGTGLVGAPECGDVMRLQ
                     IEVDEESGVIKDAKFKTFGCGSAIASSSLATEWLIGKSLEDAGKIDNMDIVEELALPP
                     VKIHCSVLAEDAIKGAIQDYKEKNGLTA"
     gene            complement(6299..7522)
                     /locus_tag="PLE23_04355"
     CDS             complement(6299..7522)
                     /locus_tag="PLE23_04355"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HMX82528.1"
                     /translation="MSNKKLIYLDNNSTTPCDPRVVEAMIPYFYEHPGNAASRNHPFG
                     WEAEGAVDLARERIAKLLAVDPKEIIFTSGATESDNLALKGVFEMYKSKGNHIITATT
                     EHKAVLDTCKKIESMGGEVTYLDVQADGLIDLAALEAAIKPNTILISIMWANNETGVI
                     QNMKAIGEICAKHGILFMSDATQAVGKIQVHPKEVGVHLMAFSAHKMYGPKGVGALFV
                     NRKGPRVKVTAQMDGGGHERGMRSGTLNVPGIVGFGKAAEIAMNEMEADFKRVAKLRD
                     KLEQALLAKLEESYINGNTDHRMPHVTNISFKHVEGEGLMMTFNQEIGVSSGSACTSA
                     SLEPSYVLVALGLGDDLAHSSIRFSLSKFTTEDEIDYAIDAVAKGVNHMRDLSPIWEM
                     YKEGVDLTKIVWDNH"
     gene            complement(7717..10038)
                     /locus_tag="PLE23_04360"
     CDS             complement(7717..10038)
                     /locus_tag="PLE23_04360"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMX82529.1"
                     /translation="MQIVKFVKKLSWKMWILIGAAFLFILVMGIGLLTYWGIFGELPN
                     YAELKNIRHSNASEVISDEGVILGKFYIENRTTVSLKNVSHYLTDALVATEDSRFFEH
                     NGIDWRSMGRVMIKSILLQKESAGGGSTITQQLAKNLFPRKRYWFGSILINKWREIIT
                     AIRLEKVYTKNEILNLYLNTVPFSDNIYGIEVACRRFFDTTTKDIKPEEAAVLIGMLK
                     GNYIYHPKLFPDRALARRNTVLDLMAKQNFLKPDECERLKKLPLKLKYTKEDQDEGLA
                     TYFRENLRLEVSKLLKGKTKPDGQPYDIYRDGLRIYTTISSRLQRYAEVAVKSHMSDL
                     QKRFEQSWGKKRPWETEENLSKWIKNTKRYQNMEAEGLTDTEIRQAFDKKIPMTIFHW
                     DGDKDTIMSPLDSLKYYNMVLNVGMLAMDPTTGYIRVWVGGSDFSFFKYDHVKSNRQA
                     GSTFKPLLYTAALEQGIEPCTWFADTATTYSEFEEWRPENADDKHRGYYSMPGALAYS
                     KNTISVQLIFRTGVKNVIDVAGRMGITTPIPNVPSIALGTADVSLWDMVTAYGTLANR
                     GNKPTPRYLKKITTKDGKVLVDFEIDKAQPTESVIGSDICDMMNQMMMGVVNEGTAKK
                     LRTQYGIVGQVCGKTGTTQDNTDGWFVGYKPKLVTGVWVGADNPIVKFRYMGQGQGSS
                     SALPIWGLFMHKVQNDKDYYKISHDTFQRPAAEFAARMNCELRVDEIPKDSTANETII
                     DKIKNVLGIGTKEKEEAEEKVQKPEAILPKKEK"
     gene            complement(10103..10828)
                     /gene="recO"
                     /locus_tag="PLE23_04365"
     CDS             complement(10103..10828)
                     /gene="recO"
                     /locus_tag="PLE23_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMX82530.1"
                     /translation="MLINTQGIVIKTIKYGETSVITHLFTRLGGLKSFIVNGVRSKRS
                     TMPFGLFQPGTLLECTMYHHDQKKLLRLKEARIAQAWQKIPFDIKRSSVALFICELLE
                     KTIRDADGHETLYDDTIAYLHYIDTTQQSLANLPLHYLIDLSHHLGFGPQGEYTEHNL
                     FFDLQEGYFMSSPHSLHYIDKETSPYLSQLLVQNYSQQGLFTMSREVRRVIFDHLLKY
                     YSLHIENFPALHSPEVFRSVMEL"
     gene            11406..12239
                     /locus_tag="PLE23_04370"
     CDS             11406..12239
                     /locus_tag="PLE23_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82531.1"
                     /translation="MKKTLSILIMMIAFVFNMNAESMNDIGRCRLINLQPVDYSSNIE
                     KSEADLLSGIWILGSNPDSDVNQKFIFEKNGKLFIFNETTERGITVDEKKWGLMDFEG
                     EVYLVFEGKSKGKDELFRMNQTCDGMELKHVKTGKLMAFEYKSLKQSAINKSELVGNW
                     TTTQYPFEIAQRFDQPGTFEPVRGAFVSLDLNDNGSYTRSYGGEVKSFSEHGTWTLSR
                     DGKYIVLASSNRKFGENNLQAIEIQKLNNNKLVVRSAISADNLTAYTTGTKLFHFAKK
                     S"
BASE COUNT         3727 a         2478 c         2604 g         3788 t
ORIGIN      
        1 gtataacaat attcatattt ttacaaaaaa aagcatggaa aaactcgaac acataggtat
       61 agcagtcaaa gacctcgctg tgagcaatca actttttgag aagatactcg gggcgccaca
      121 ttacaaaatc gaagaagtca cctcagaata tgtcaatact tcctttttta aaatcggaga
      181 aacgaaaatc gaactgcttg aagcaacgca ccctgatagt gccattgcaa aattcattga
      241 aaagaagggc gaaggcatcc atcatctggc attcgaggta agtgacattc acgaagcaat
      301 gctgcgcatg gaagaacagg ggtttacctt attgaacaaa acacccaagc gaggtgccga
      361 caacaagctc atctgctttt tgcacccaaa gactaccaac ggggtactca tcgaattatg
      421 tcagtcaata gaggaataga atgaatacac accagtcggc atacgaggca gcatttgcaa
      481 agcttaacga agggcagcga caggccgtgt tagctaccga aggaccggta atggtattgg
      541 caggccccgg aacaggaaaa acccaggtat tagctacccg aatcgggtat attcttgaca
      601 atacggatac caacccacag aatatccttt gcttgacatt tacagatgcc ggcgtggttg
      661 ctatgcgtga gcgtttggtc aaattcattg gcaaagaagc gtataaggtg aatatccaca
      721 cctttcattc gttttgcaat atggtcattc aggcaaatac ggagtatttt acccaaaatt
      781 ttcagctaaa tgtggccgat gacttgcaga aaaaagaaat agtgcgcaag atgatagacc
      841 tgctgccggt ggatgacgtc aataagcgcc tgaagaatgc attttatctg gatgaaaata
      901 aatacatgag tctgttcagt gagatgaagc gtgaaaacat ttcaacagag caaatagaag
      961 ctgacggcca attcgtgatg caaagggtcg aagactttga agacatggtt tatttgagaa
     1021 aatctggcga aaacaacaaa ggcgacaaaa aactcaagga tattgcaaaa atacgagaag
     1081 gtgttggtaa gctggtggct gcatccagac aactggaaac ctacaatgcg ctccttgcag
     1141 aagatggttt gtacgactac gacgacatga ttaactgggt agtcaatgct tttgaacgcg
     1201 acgaaaacct cttgcttgac tatcaagagc aatatctgta catcatggtc gatgaatttc
     1261 aggacaccaa cggcatacag atcaaattgc tgaaccagtt gtgcagtttt tggggcgaca
     1321 gcccgaatgt atttgtggta ggtgatgacg accaggcgat ttaccgtttt cagggggcca
     1381 atgttgataa tcttagctca ttttacgaaa aatataaacc cgcaatcgtc tgtcttacaa
     1441 aaaattaccg ttcaaaaccc gacataatca caacggccaa gaatctgatt acaaataaca
     1501 agtcgcggtt gggtagtgct ataccgggtg tcgttaagga catcttgcca ggcaaagagt
     1561 gtgaagacaa cggcagtgta gcggcatttc aatatgccaa taacgaacag gaagatacag
     1621 ccatcatttg ttggatagaa aaccatatta aggcaggagt gaaacctaag caattggctg
     1681 tagtgagcag aaatcatgac gggcttaggg agctggtgaa tattttacag aaaaagcaga
     1741 tacctgtgtt tgtaaagaca aaggtgaatg cattgcacat tccggttgta aggaatcttg
     1801 ttaccatctt gcgttttcta gattcttgtt tacgcgaccc ctatgatgcg catatatgtc
     1861 ttgccgaaat catacagtat ccaaaccaag gaatacaatc atttgatgtg ttgaaactga
     1921 taaggtatgt agatgaaata aatttcaata aagaaaataa agaaaaaatc tggctggccg
     1981 aaattatcgg gtctgaaaag catcttcaag cagccggagt gtcggatgcg gatgttattt
     2041 tatctctcag tagaaaaatt gaaaactggc tggcagctgt tcataccgaa acgatacagg
     2101 tattatttga aaaaattctc tccgaaagcg ggatccttga atttgtactg aactcggcca
     2161 ataagatcaa tcttttggaa gccatacatg ttttgtttaa tttcatcaaa gaaaaaacaa
     2221 cagtagatga ctctttcaac ctgcatagtc tggtagtgtt aataaaaaaa atggaagaat
     2281 tgaagattga tctgccattt attaagatta ccggcaactc agaaggtgta cagatgatga
     2341 cagcacatgc taccaaaggc cttgagtacc cttacgtatg gattaaaggt gccgatgctg
     2401 caaaatggga agtaaaaaga ggttcttccg gcagttttat gagcggattg tttaaagcta
     2461 tactacgcat tccggatgtg gccagaaaga aagatgctga agaatggtca aaagatattc
     2521 aactcgaaga cgaacgaagg ttgtttttcg tagccactac cagggcagaa gaggaactga
     2581 tcatgtcata ttcgcccaaa acttcggata aaaaagaaac tccttcaagg tttttgatag
     2641 aatcgatgca gacagacgaa ataccgacag ctgaagtgaa tcagacggca cttgtcgaac
     2701 atcttgcact gaaaatcaga aaatcggaac tcgaccaaga aaacgtagaa cgcgaatacc
     2761 ttgaccgcat acttaaggat ttcaaattaa gttctactgc attaaataac tacctcaagt
     2821 gtccacttcg tttttattat tccaatatca tgcgcatacc tcaagccagg agtgccggta
     2881 tgggattcgg caatgtggta cataaagtgt tggagcgatt atggaagtac cagaaagaag
     2941 caggtggctg ggatgcagtt cacgatcttg atacacgtat cgtacaatac atcgaagaag
     3001 ctatgaagca gttccagtcg cattttaatc cgacagaact gaagaacaat tttgaagaag
     3061 gaaagaaaaa attaccctta tttatcaagt cacaattggc agcctggcgc aatataccag
     3121 atttcaagac agaatataag atacagacga ttcttgacgg aaaaattcca ttgaccggaa
     3181 atcttgaccg tttagatata ctcaaggacg acatccatgt taccgactac aaaaccggaa
     3241 gtctcagacc cgaacaattg aagtcgggcc tggcagacga caaaggaatc ggaggtgatt
     3301 actggcgaca gatggttttt tataaaatcc tggtggacct tgataatagt ttctcaagac
     3361 agatgacttc cggaacgatg caatatgtca ccgatgagaa gaatggtgtt ttttcttccc
     3421 agaaatttac cattacacct gaagaaatac aaatggtgag cgaacagata gaggacacgt
     3481 acgcaaaaat cctgcgtcac gatttctaca aaggttgcgg taaacaagac tgccactatt
     3541 gccattttgt aaacgatata aagtcgctaa gcttggcaga tgaaagcgat gccgaataca
     3601 gtaacggaga tgattttgca tcaccgcaat ccgatgagta aaaggaataa ctattaaagc
     3661 tgaaaaatac tatgcacctt cagcattatc agcctttagc tgtgcagcat tgattgagct
     3721 tatttcctga tcgatgtaat acaatgacat tgggccactg cctatgagtt tgattttgtc
     3781 caggatggag cgcattagtg cttcttcttc gcgttgttcg gttatgtacc actgaaggaa
     3841 gttgagtgtg gtataatcat cttccttatt actcagctct accaggtggt tgatggctct
     3901 ggttacacct ttttcgtgct catatacttg ttcgaagagt ttttgtacag actcgaattg
     3961 atgtggtggt tgtgcaatgg ctggcgtgag tgcatatcca tctacttctg aaatgtagtg
     4021 aaatattttg agcatgtgct ggcgttcttc atccgactgc cggtgcatga atgcagcaca
     4081 tccattcaga ccttccttat cgcaccatga tgccattgac aggtagagga aagatgcata
     4141 agcttcgagt tctatttgtt tgttgagggc ggcctccatt gtgttcgaaa tcattattct
     4201 ggttttttag gtttacattt actttggtat cagcaacaag cataccgaaa ataaaacgac
     4261 cgaaatcaat aatccgtata aaaatacatt ataacaatat gtaaggtaac ggtatttttt
     4321 gttgagtacc tgaccgagga agtaaatact cttggacatc gaatcgtaaa tatattgctg
     4381 gtcctgtata agctggcgca tagcccattg ataatcgggg agtttcatgc ctgtatagtt
     4441 gccaaaaaat aacagatcgg ctgttttttc ttttacttca tccagtgaaa cattgccttt
     4501 tatcagcttg ggtttgatgg tgagtatggc ataaaatacc gacaacagac atgcgccaag
     4561 taagaaaatt gtagggatga tgaggcggta atctgtctgc catttgggga ttaggttaga
     4621 gcctaccacg gagaaaataa tggaattgac acccatcatg atatgagcct tagagtctgc
     4681 aagtgtgttg agattcagat ggtggttata cgatgttctg aaaagcgtat ctgacccacg
     4741 cgatagtttc ttttgctcaa aatcaaagtt ttctaccgga tttggttgtt ctgtgctatt
     4801 cttttctttc cgtttattca gttcataata cagttgtttc aggaagagtt gtttgttatt
     4861 ttcaaataaa tatctgcccg aatcggtatg gtaatgatgg ttggcaataa aattaatttc
     4921 gaactgcaac cattcatttt tattcatttt aatgccgttt gcctccattt cggctttaag
     4981 caaatcattt ctctcccaaa aatgttcatt gcctaaatgt gccaggtcgg catcacatat
     5041 aatccgttcg ttgagtgttt tgggcgattg gggcattttt gtggccataa tgatatcctt
     5101 aatgaccata atatgctctt cagaaagctt gccttttaaa aaattctccg ctaccaaaac
     5161 acttcgggct tcgtgcccca ttccaccctc tgtataaccc gtgtcatgaa accaagctgc
     5221 aattttgagc aaatgcatat cttgagtgga taaaccttct ctttcaccaa taaggtatgc
     5281 tgagtgtact acctgtgcag tatggtcaaa attgtgaaaa gaatattgtg cgggcatatt
     5341 tttttgaaaa tattcccgga taaaattctc ggattcacgc aatacaggtt ctaatggctg
     5401 gtacatagaa tgcaaaattg ctaaaattta agtaatcttt ccttttttgt aggataaaaa
     5461 aaagaccgga tgtgtacacc cggcccaaca ttattactaa cacttaaatt ttttaatatt
     5521 acactgcaaa actttcacca caaccacatt cgcgtgaagc atttgggttg cggaaaaaga
     5581 atcctttgcc ttcaagacct cctgagaagt caagtgttga gttgaacaga tataggaaag
     5641 atttaaggtc ggacactatt tttactccat tatcttcaaa tatctggtca ttaggttgtg
     5701 gttcgttatc aaaatccatt ttgtacgaca ggcctgaaca accaccggag ataacactta
     5761 cacgcacgaa atagtcatcg gtgatatgac cgctttctct gatttgcttg atcttttcct
     5821 ttgcgctatc agctacgaat aacaatgatc cttccatttt tttaagcagt taagccgttt
     5881 ttttctttat aatcttgaat ggcacctttt attgcatctt cggccaatac tgagcaatga
     5941 atttttacag gtggcaatgc aagttcttca acaatatcca tattgtcaat tttacccgca
     6001 tcttcgagcg atttgccgat gagccattct gtggccagac ttgagcttgc aatagcagag
     6061 ccgcagccga aggttttaaa ctttgcatcc ttgatgacac ctgattcttc atctacttct
     6121 atctgcagtc tcattacgtc gccacattca ggagcaccca ccaggccggt gcctacattt
     6181 ttcttgttct tgtcaagcgt gcctacattt tttggattct tgaagtggtc cagaactttt
     6241 tcagaatatg ccatttttca atttatttta aagcgtaatt ttaaaaatat ttatagtatc
     6301 aatgattgtc ccatactatt ttcgtaaggt caacaccttc tttatacatt tcccatatag
     6361 ggctgaggtc tctcatgtgg ttgacgccct tagctacggc atctattgcg taatcaattt
     6421 cgtcttcagt tgtaaatttg ctaaggctga aacggataga ggagtgtgca agatcgtcac
     6481 ccaaccccaa tgcaactaac acataagaag gttcgaggct ggcagaagta catgctgaac
     6541 ccgaagaaac gcctatttct tgattgaaag tcatcataag accttcacct tctacatgtt
     6601 tgaatgagat attggtcacg tgtggcattc tgtggtctgt attgccattt atgtatgatt
     6661 cttcgagctt ggcaaggagt gcctgctcga gcttgtctct cagttttgct acgcgtttga
     6721 agtcagcttc catttcattc atggctattt ctgcagcttt gccaaagccc acaatgcctg
     6781 gaacattcag tgtacctgat ctcatgcctc tttcgtgtcc gccgccgtcc atttgggctg
     6841 ttacctttac acgcgggcct tttctgttta cgaataaggc acctactcct ttaggaccgt
     6901 acatcttatg tgctgagaat gccattaggt gaacacctac ttcttttgga tgtacctgta
     6961 tcttgcctac agcctgtgta gcatcgctca taaataaaat accgtgtttg gcacatattt
     7021 cacctatggc tttcatattt tgaatgacac cggtttcgtt attagcccac atgatgctta
     7081 tgagtatggt atttggttta atagccgcct caagagctgc tagatcgatg agcccgtcag
     7141 cttgtacatc cagataggtg acttcaccgc ccatggattc tattttctta caagtatcta
     7201 gtacggcttt gtgttcggta gtagcagtaa tgatgtggtt gcctttcgac ttatacattt
     7261 cgaatacacc tttcaatgcc aggttatctg attctgtagc accggaggtg aatataattt
     7321 ccttagggtc aacagcgagc aattttgcaa ttcgttcgcg tgccagatca acagcacctt
     7381 ctgcctccca tccgaatggg tggttacggc tggcagcatt acccggatgt tcgtagaaat
     7441 aaggaatcat cgcctctact acccttgggt cgcagggtgt agtagaatta ttatcaaggt
     7501 aaatgagctt tttgtttgac atgatttttt aattgttatt tagactattt ctaaggagat
     7561 acaaaagtaa ccactttctc agaagttttg ttcaaaaaaa taatgaaaaa ttgattttga
     7621 tagataaaat tgattttaac ggctcatatc ataaccattt ttcaatttat aaggatgcag
     7681 aattgaatat tgaccagatg ttgccgggcg gtattattac ttttcttttt ttggtagaat
     7741 tgcttcaggt ttttgtactt tttcttcagc ttcttctttt tctttagtgc cgatacccag
     7801 tacgttcttt attttgtcaa taatggtttc gttggctgta ctgtccttgg gtatttcatc
     7861 cacgcggagt tcacaattca tccttgctgc aaattcggct gcaggtctct gaaaggtatc
     7921 gtgcgatatc ttgtaataat ctttgtcgtt ttgtacctta tgcataaaaa gtccccagat
     7981 gggcaaagca cttgatgaac cctgaccctg acccatgtac ctgaacttta cgattgggtt
     8041 atctgcacct acccaaactc cggttacgag ctttggtttg taacctacaa accaaccgtc
     8101 tgtgttgtct tgtgtggtgc cggtttttcc acacacctga ccaacgatgc cgtattgggt
     8161 gcgtagcttc ttggcagtac cctcatttac cacgcccatc atcatctggt tcatcatgtc
     8221 gcagatatcg ctgcctatta cactttctgt tggttgtgcc ttgtctattt caaaatcaac
     8281 caatacctta ccgtctttgg tggtaatctt ttttagatac ctcggagtag gtttattgcc
     8341 tctgttggca agtgtaccgt atgctgtcac catatcccat agcgaaacat cagcagtgcc
     8401 gagtgctata gatggcacat taggaatagg ggtggtaatg cccattctcc ccgcaacatc
     8461 aataacattt tttacgccag tacggaatat taattgtacc gatatggtat ttttagagta
     8521 agcaagtgca cccggcattg aatagtaacc gcggtgtttg tcatcggcat tctccggccg
     8581 ccattcttca aactcagaat aggtagttgc cgtatcagca aaccatgtac atggctcgat
     8641 accctgttca agtgctgcag tgtagaggag tggcttgaat gtagatccgg cttgcctgtt
     8701 cgacttaaca tggtcgtatt tgaagaatga aaaatctgat ccacctaccc acacacgtat
     8761 gtatcctgta gttgggtcca tagccaacat acctacattg agtaccatat tataatattt
     8821 aagcgaatca agtggactca taatggtgtc tttgtctccg tcccaatgga aaatggtcat
     8881 gggtattttc ttgtcaaatg cttggcgtat ctcagtatcg gtaaggcctt ctgcttccat
     8941 attctgatag cgcttggtgt tcttaatcca tttgctgagg ttttcttctg tctcccacgg
     9001 tctttttttg ccccacgact gctcaaaccg cttctggagg tcggacatgt gtgatttaac
     9061 ggccacttcg gcatatcgct gtagccgact acttatggta gtataaatgc ggagaccatc
     9121 tcggtaaata tcatagggct gtccgtccgg tttggtcttt cctttgagga gtttgcttac
     9181 ttcgaggcgc agattttctc tgaagtaggt agctagacct tcatcctggt cttcctttgt
     9241 atatttgagt ttcagtggta attttttcaa tcgttcacat tcatctggct taaggaaatt
     9301 ctgttttgcc atcaggtcaa gtacggtatt gcggcgtgca agtgcgcggt cgggaaacag
     9361 ttttgggtga taaatgtaat tgccttttag cattcctatc aatactgcag cttcttcggg
     9421 tttgatgtct ttggtcgtag tatcaaaaaa acgccggcat gccacttcta taccatatat
     9481 attgtcagag aaaggaacag tattgagata tagattcaag atttcgtttt ttgtataaac
     9541 tttctccaat cgtatagctg taatgatttc gcgccattta ttgatcagaa tagagccaaa
     9601 ccagtatctt tttcttggaa ataaattttt cgccagctgt tgagtgatag tactgcctcc
     9661 acccgctgat tctttttgta gcaagatcga tttaatcatt acacgtccca tacttcgcca
     9721 gtcaatacca ttgtgttcaa aaaagcggct gtcttcggta gctacaagtg catctgtaag
     9781 gtaatgagag acattcttca gacttacagt tgtacggttt tctatataaa atttccccag
     9841 tatgacgcct tcatctgata tcacttctga tgcattgctg tgtctgatgt tctttaactc
     9901 ggcataatta ggcaactcac caaagatacc ccaataggta agcagaccga tacccattac
     9961 caatatgaac aaaaatgcag ctccgatgag tatccacatt ttccatgaca acttcttgac
    10021 aaacttgact atctgcatgc ttaatcgaaa tattagcaaa ttaacgaaga aatttccggt
    10081 ggtattatat tttctctgat attcataact ccatcaccga ccgaaatact tcgggtgagt
    10141 gcaatgcagg gaagttttct atatggagtg aataatattt gagcagatga tcaaatatca
    10201 cacgccttac ttcacgcgac attgtaaata atccttgttg cgaataattc tgtacgagca
    10261 attgacttaa ataaggcgag gtttccttat caatatagtg caaagaatgt ggtgagctca
    10321 taaagtaacc ttcctgaaga tcaaaaaaca ggttgtgttc ggtatattcg ccctgtgggc
    10381 caaaacctaa atggtggctc aggtctatca ggtagtgcaa tggaagatta gccaaggact
    10441 gctgggtggt gtcaatataa tgcaggtaag cgatggtatc atcatacaaa gtctcatggc
    10501 catcagcgtc tcggatggtt ttttccagca attcgcagat gaacaatgca acagagctac
    10561 gctttatatc gaatggtatc ttctgccagg cttgtgctat gcgtgcttct tttagtcgca
    10621 aaagtttttt ttgatcatgg tggtacatcg tacattcgag cagagtaccc ggctgaaaca
    10681 atccaaaagg catagtgctc cttttagaac gtacaccatt tactataaaa gattttagcc
    10741 cgccaagtcg tgtaaacaga tgagtaatga cacttgtttc accatatttt atagttttta
    10801 tgactatgcc ctgtgtgttt atcagcattg tttgtatcag agaatatttt tcgaatggca
    10861 aatttaccta ttagctgtgg caggtacaaa ttaaaaacga gttttactga gcactatttt
    10921 gttaatgaat tttaaagcaa atcaagaaag caactttata aaaaataatt taaaaaaaag
    10981 cttgaaaaaa atctgtcatg caccaaaatt gaattttagc attttcgcaa tgaaacagaa
    11041 tataatttgg ttataggctc aaatatggca aacagaaaaa atttaataaa aaattaacgc
    11101 tgttttgcag aacaaaatac ttttttgttt cgttactatg ttgtagcttt gcagcgaaat
    11161 tgattcgaag gaattcgatt tgtttacaaa cacactgaag agaaaacctg acatacatat
    11221 taaggtatgg cgatgaatat gtaggtcagg attgctaaga taccgttggt cggtaaaacg
    11281 taaaacttca tcggttattt tagcaatttg agcagaagaa agaagatcgc ttgtaacaaa
    11341 tcgcattgcc cgggcgtcat tagggcaaac gaataaattt taaaaaagaa cataaaacga
    11401 aagttatgaa aaagacatta tccattttaa ttatgatgat tgcattcgta tttaatatga
    11461 atgcagaaag tatgaatgac ataggacgtt gcagattgat aaacttgcag ccggttgatt
    11521 attcatctaa tattgagaaa agtgaggcag acctgttgag cggaatctgg attctaggta
    11581 gcaatcccga ttctgatgtc aaccagaagt ttatcttcga aaaaaacggc aagctattta
    11641 tattcaatga gacaaccgaa cgcggtatca ctgttgatga aaaaaaatgg gggctgatgg
    11701 attttgaagg tgaagtttac cttgtattcg aaggtaagtc aaaaggtaaa gacgaattat
    11761 tcagaatgaa tcagacctgt gacggtatgg aacttaaaca tgtaaagacg ggcaaactga
    11821 tggcatttga atacaaaagc cttaaacaat ctgccattaa caaaagcgaa cttgtaggca
    11881 actggactac tacccaatat ccattcgaga ttgcacaacg ctttgaccaa cctggtacat
    11941 ttgaaccggt aagaggagct tttgtaagtc tcgatttgaa cgataacggc tcctatacaa
    12001 gaagctacgg tggcgaagta aagtcattct ctgagcacgg cacctggaca ttgtcaagag
    12061 acggaaaata cattgtgctt gccagctcaa accgaaaatt cggcgagaac aatctgcagg
    12121 caatcgaaat acagaaattg aacaacaaca aactcgtggt gagatctgcc atatcagcag
    12181 acaaccttac tgcctatact acaggtacca agttgttcca tttcgccaaa aagtcataag
    12241 cgtttttttg atcatactat tcgggccgtt tttcgcaaag agaaacggct ttttttattt
    12301 taaaaaatat tggactgcac tcgtattaat atccggtctt gtgtcatttt catttcatgg
    12361 agtacattat tagtattaat aatatgtgta cttgatctgt atgtacttaa tattatattg
    12421 agtattgcaa gcggcttaac atgcacatta tcaatcatct cagcttgtat agctcgtatt
    12481 tatccagcaa actgacagaa aacaatattg tggaagccat tatttgccaa attaaaattt
    12541 taaaatggca cttatttttc attgatacct aattttattt ggttttaatt aaatgga
//
LOCUS       DAOQQY010000054        12017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1864_length_12017_cov_6.068467, whole genome shotgun sequence.
ACCESSION   DAOQQY010000054 DAOQQY010000000
VERSION     DAOQQY010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 12017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12017
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1864_length_12017_cov_6.068467"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(213..542)
                     /locus_tag="PLE23_04375"
     CDS             complement(213..542)
                     /locus_tag="PLE23_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82532.1"
                     /translation="MAANDTRTEKNIQSNKGIIESLFSPLGFTRTIVKHQQYEQDNYI
                     RSMMDYFGTANVHVLPFIYQPQQINERASMSFHLTQREKADLHRSLSTPENIASFRRY
                     EQLFKDR"
     gene            complement(576..2423)
                     /locus_tag="PLE23_04380"
     CDS             complement(576..2423)
                     /locus_tag="PLE23_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82533.1"
                     /translation="MKDKIRDFWYSFPVQLTILHFRNNLSLLLLWLLTIVFVTGYRGK
                     LFGLQYLFTDPEYMGKVGPASFGFLGVAFGVFVMVWHMSSYLLSAFRFPFLASLARPF
                     SKFIINNFTFPVIGFVAILSSIIYLQRFVEGKSWMEVAGYIIGWVSGVMIVIALLTLY
                     LVATNKDYRTYTRHLKQHETEVFYTDPTELAYQKYASSERRVHTYLNESLQPRLVRNV
                     AHYPYTVLQKVYKQNHKNAIILLIIGIVVLISLGLLSDNRYFRIPAGASILLLFSVLL
                     SIVSAISFWFRNWRLQVFVVLILLANFITKNDRLLHFNQAYGIDYQKTPLPYDPDSCG
                     VQYDDLKYANDKKSGFGVLEKWLDKQKSEKPYAVFLCASGGGQRAGVWAMEVLDSLNQ
                     MSTNELLDRTVMMSGASGGVLGMAFFRAIFENKRLLDPTYPYKTDFSKDMLNSLSFTL
                     VSNDIFPNFSRFNYSGKTYFRDRAYAFEQQFNENTSGFLHRNLASFREAESQAQIPML
                     FITPTIINDGRRLIISPLDVSYMTQAPYHADSTRLPKDGIEFRDYFRSMQADSLSFLS
                     ALRMNCTYPFILPNVFLPTIPAMEVMDAGFNDNFGISVAYRYIWNYKEW"
     gene            2592..3662
                     /gene="prfA"
                     /locus_tag="PLE23_04385"
     CDS             2592..3662
                     /gene="prfA"
                     /locus_tag="PLE23_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HMX82534.1"
                     /translation="MLTKLEAIFDKYMRLEEQLSDPEIISDMSRYSKVNKEYKDLKPL
                     IECYKQYKDLVGNIATARAMLSESDLEMKEMAREELQLLEPEKEELEEKIKMLMVPKD
                     PDDDKNVIFEIRSGTGGDEASLFAGDLYRMYSRYFEKQAWKTEVISVNEGTVGGYNKL
                     VLGVSGEGVYGKLKFESGAHRVQRVPDTEAKGRVHTSAATVAVIPEYEEDDITINKAD
                     LRIDTFRASGAGGQHVNKTESGVRITHIPTGIVSESTEDRSQHKNRDIAIGRLMFEIK
                     ETQRKKFESEQAAKRKSLVGSGDRSDKIRTYNFPQNRVTDHRIDLTLYKLDQVLEGDV
                     DEIIEALVVYENATKLMEMEGV"
     gene            complement(3746..6412)
                     /gene="cphA"
                     /locus_tag="PLE23_04390"
     CDS             complement(3746..6412)
                     /gene="cphA"
                     /locus_tag="PLE23_04390"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HMX82535.1"
                     /translation="MKILEINAMRGPNYWSIRRHKLIVMRLDLEDLEDRPTNKIPGFL
                     DRLKELLPSLYEHRCSEGYAGGFYHRIEEGTWMGHVIEHIALEIQTLAGMDVGFGRTR
                     ETGVPGQYHVVFAYLEEEVGRFAARAAVRLAQALVDGEKYDLASDIQDMRELRERNRL
                     GPSTDSIVQEAISRGIPWIRLNKYSLCQLGYGANQKRIQATVTSQTSSIAVEIACDKE
                     DTKTLLEAAEVPVPRGEIIKTEAGLKDAVSYVKYPLVIKPINGNHGRGITVNINSWEE
                     AVTAFEAAKRVSNSVIVEKFVTGEDYRLLVIDYKLVAAAKRTPARVIGDGKHSIKELV
                     DIVNSDPRRGYGHEKVLTQIDFNDLTMTIIHSKGYTLDTVLPDGEILNLKDTANLSTG
                     GTAEDVLDAVHPYNVFMAERIARLIGLDICGIDIMTSDISQPLTDTGGAVLEVNAAPG
                     FRMHLAPTEGLPRNVAANVVDMLFPPGATARIPIVAVTGTNGKTTTTRLIAHMVKMMG
                     HRVGFTTTDGVYIQNHLMMSGDCTGPASAEFVLKDPTVNFAVLECARGGILRAGLGFH
                     SCDMAIVTNVAADHLGLKGINTVEQMARVKGVVVESVRPEGYSILNADDDNVYNMRNN
                     VQSKVALFSMDENNPRIKEHSKNGGISALYENGFITICKGEWKIRVAKAVNIPLTYGG
                     RAGFMIQNILPSVLVGFLNNFKIEDISTALESFIPSPSTTPGRLNWFNFKNFDVVLDY
                     AHNPAGLRALQHFINKIDNTPKVGIIAGIGDRRDEDNMEIGRICAEMFDELIIRQDKH
                     LRGKKDHEIIDLLLAGIHQHDANKPVTIIPKESEAIRYAIEHAKKASLIVICSDVVTE
                     ALNLVQQFKEEEANKLYDYKNVEIPNIEDIGH"
     gene            complement(6607..9390)
                     /locus_tag="PLE23_04395"
     CDS             complement(6607..9390)
                     /locus_tag="PLE23_04395"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX82536.1"
                     /translation="MKKLIVLCLMAVVTANVIAQNQDKTTILDAQTGEPLPLVLGGLL
                     NPVDSTIIRAIQADDNGVIHHNATEKTDVIIQMKYLGYDTYSMFLAKDTPWPATIRLN
                     KSGITLKDVQVTGEKVAVQVKNDTVEFNASTFKTRPNDMAEDLLKKLPGVQVENDGTV
                     KNQGEQVTKITIDGKPFFGNDPKLATKNIPADAIDKVQVFERKSDQSTFSGFDDGNTE
                     RSINFTLKPDKRKARFGRVETGVGTEERYESSGTLFDFNQGRQLSVLAMSNNANKSGF
                     TSEDAMSFSNASGGSQGGGPGGPGMRGPSIGGQSIFSLLGSSNLGINRNTTIGTNFRD
                     TWGKVEFTGSYFFANTGNRSESQTHRQTFLEGNTLLSDGSGNANTTGNNHRVNLGFDY
                     AINKNNSIKFEPNLSFSDNNSSSLSLSKQRNSDGASLNDVNAKVDAASKNFRISGDLL
                     YRHKFAQEGRTLSLSVSPKYSSNNNTTNNQSVSQIFTDLSYTTDSLNQKVVNYQNGNG
                     LGSNLSYTEPLSKAISMELSYNYNIEKNARNRESFEYNPATGAYDIFQDTLSNDFNNT
                     YETHRPQISLQYKLEKLSVTVSGSVQRASLRSESVSDNLPFSKSFTNFLPGLHARYTL
                     SKNKSIMLFYRTSTRQPAIDDIQPVPDLSDPLRIKLGNPNLGQEYRHNMRLSYRIFDM
                     VSNTFLSFGVRGEYTGDKIVSNVFIDQSGVEITNKENVDGVYSGSFFANYGRPFRKIN
                     LNVSSSTNYRRDVSFVNKERNNGDVWSTSGRLYVNYNITDAWDFDLGGSVAYNEAKYS
                     LNPRLNNNYTDINTEFNTTADLPFRFRVSTNINFTQRQGLAGDFTKNFTIWNASLTKT
                     FLPNNSFELSLSAYDILKQNTAVNREVTSSYIEDYQALSLSQYFMLTARYYLNKQAPT
                     MSKTGKRFFRMMH"
     gene            complement(9426..10136)
                     /locus_tag="PLE23_04400"
     CDS             complement(9426..10136)
                     /locus_tag="PLE23_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82537.1"
                     /translation="MKKIKLLILLCFVTSITFAQQTKLATIRYDIVRETGIPGRKAAE
                     GTEKLSNTITEDRFVYFKDTLAYYERTKGGGKMFKNQPGVKFKLPFEEYTFTDMKNHK
                     EIISLTVLNESNEMYYSVEALPRPKSWKDTGKTQKMLGLKCYNAKVVNEMGEYDIWYT
                     KELDFNFSPLPGIQPAKGLVLKIEGPQVYYNARIIEKKLDNPGVFNKIYKGEQVTKED
                     LLKVRSHSSGKLRPAKSK"
     assembly_gap    10500..10599
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10773..11111)
                     /gene="cas2"
                     /locus_tag="PLE23_04405"
     CDS             complement(10773..11111)
                     /gene="cas2"
                     /locus_tag="PLE23_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HMX82538.1"
                     /translation="MDSLRFNAYRNMWIFVLFDLPAETKKERSDAAKFRKELLERGFN
                     MFQFSIYLRFCQSMENAETHKKYVKSILPEKGKVGVLTITDKQFGCMELFFGTKKESV
                     TPPPQQLTLF"
     gene            complement(11120..>12017)
                     /gene="cas1"
                     /locus_tag="PLE23_04410"
     CDS             complement(11120..>12017)
                     /gene="cas1"
                     /locus_tag="PLE23_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HMX82539.1"
                     /translation="LVMVKRTLHFSNPAHLSVHNSQLIVELKDEGRTRHTVPIMDLGM
                     VVLENPQITISSAVLEAFMEENVALISCNSKYMPSGMFLPFDANTTQTKVMTTQVEAS
                     MPLKKQLWQQTVKAKIANQAILLDKLNLNSRRLHVLKEQVLSGDTTNCEAQAAGHYWN
                     QLYGTEFIRSQERDTPNAQLNYGYAILRSVIARALAATGLHPSLGLFHKNKYNAFCLA
                     DDIMEPYRPYVDWLVFGLIKQHPSDGLTREQKIELLKLPQIDVTIGKITRPLFHAVSM
                     TTAGLNRCFAGDIRKIPFPVFK"
BASE COUNT         3218 a         2498 c         2647 g         3554 t
ORIGIN      
        1 tgagggatag aaggcggcac aacgtaagcc ggatagcccg acccgacagg ggcacaccct
       61 gaaaataaac ccagttaacg ctttaggctc ttgactgcca atcatgagta ctacaaatct
      121 gtttgaaaag cttaaaaacc ataggtcact ttgctattat cactttatta tgcattcttt
      181 ggactaaagg ttttgtatgg attcctaatc cgttatctgt ctttgaacaa ctgttcatac
      241 cttctgaagc tggctatatt ttcgggtgtg gagagtgagc ggtgaaggtc ggctttctca
      301 cgttgtgtga ggtgaaagct catggatgcg cgctcgttga tttgttgtgg ttggtagatg
      361 aacggaagta catgtacatt ggctgtgcca aaataatcca tcatagatcg gatgtaattg
      421 tcttgctcgt attgctgatg cttgacgatg gtgcgagtga aacccagcgg actgaaaagg
      481 ctctcgatga ttcctttgtt tgactggatg tttttttcgg tgcgggtgtc attggctgcc
      541 atctggatga tgatgatacc ggcggtgttt tctttttacc attctttgta gttccagatg
      601 tagcggtaag caacactgat accaaaattg tcattgaatc ctgcatccat cacttccata
      661 gcaggtattg tgggcagaaa aacattcgga agaataaagg gataggtaca gttcattcgc
      721 agggcactta gaaaactcag actgtcggcc tgcatgctcc taaagtaatc cctgaactcg
      781 ataccgtcct ttggcagacg agtgctgtcg gcatgatatg gtgcctgggt catataagaa
      841 acgtcgagcg gtgaaatgat aagccttcgt ccgtcattga taatggtagg tgttatgaaa
      901 agcatcggaa tctgtgcctg cgattctgcc tcacggaaag aagcgagatt gcgatggagg
      961 aaaccagaag tattttcatt gaactgttgt tcaaatgcat aggcccggtc tctgaaatat
     1021 gttttgcctg aataattaaa tcttgaaaaa ttgggaaata tatcattcga gaccagagta
     1081 aatgatagtg aattgagcat atcttttgag aagtcggttt tgtatgggta tgtagggtcc
     1141 aacaggcgtt tattctcgaa tattgctcta aagaaagcca tgccaagtac accaccggaa
     1201 gcgccgctca tcattactgt tctatcaagg agttcgttag ttgacatttg attgaggcta
     1261 tccagcactt ccatagccca gacaccggcc cgttgaccgc cacctgatgc acagagaaac
     1321 acagcatacg gtttttctga tttttgtttg tcaagccatt tttcaagcac tccgaagcct
     1381 gattttttat cgtttgcata ttttaaatcg tcatactgta ctccacagct gtcaggatca
     1441 tacggaagtg gtgttttttg ataatcgata ccataggctt ggttaaagtg gaggaggcga
     1501 tcgtttttag tgataaagtt ggccaggagt atcagtacca caaatacctg aagtcgccag
     1561 ttgcgaaacc agaatgaaat agcactgaca atagacagca gcacactgaa cagaagaagg
     1621 atgctggccc cggcaggaat tctaaaatac cggttgtcgc ttaggagccc taatgaaatc
     1681 aagacaacaa taccgattat aagcagaata atggcgtttt tgtgattctg cttataaact
     1741 ttctgtagca ctgtataagg ataatgtgct acattgcgca ccagacgtgg ttgtaaagat
     1801 tcattgagat aagtatgtac acgtctttct gaggaagcat atttctgata ggcgagttcg
     1861 gtagggtctg tatagaatac ttcagtttcg tgctgtttga ggtgtctggt gtatgttcgg
     1921 tagtctttgt tagtggccac aaggtaaagt gtcagtaggg ctattactat cattacacca
     1981 gacacccagc ctatgatata acctgcaact tccatccatg attttccttc aacgaatctt
     2041 tgcaagtaaa tgatggatga gagtattgct acaaaaccga tgaccggaaa ggtaaaattg
     2101 ttgatgataa atttgctgaa aggccgtgcc agagaagcca gaaacggaaa ccggaatgcg
     2161 cttaacaggt aagaagacat gtgccaaacc atgacaaaaa cgccgaaagc aacacccaga
     2221 aagccaaaac tggcaggccc gaccttgccc atatattcgg ggtcagtaaa cagatactga
     2281 agaccgaata gcttacctct gtatccggta acaaatacaa tggtaagcaa ccacaaaaga
     2341 agcaacgaca gattattgcg aaaatgcaat atggttagtt gcaccggaaa tgagtaccaa
     2401 aaatcccgga ttttgtcctt catgatagtt tacttggcaa gtattaagct ttcgagcccg
     2461 aaattattgt aataatacaa caaattctgt tcctttttta aaacgattga attattatta
     2521 tgttggtttg cacttattgt cggatgcctt aatgttaaaa tattttacct ttgcatttca
     2581 acttattatt tatgcttacc aaattagaag caatattcga taaatacatg agacttgaag
     2641 aacagctctc tgatcctgag atcatcagtg acatgagccg ctactctaaa gtcaataagg
     2701 aatataagga cctgaaacca cttattgaat gttataaaca atataaggat ttagtaggaa
     2761 atatcgctac ggcacgtgca atgctctctg agtctgacct ggaaatgaag gaaatggcgc
     2821 gggaagagct tcaactactc gaacccgaaa aggaagagtt ggaagaaaaa atcaagatgc
     2881 taatggtacc aaaagatccg gatgatgaca agaacgtcat tttcgaaatc agatccggta
     2941 cgggtggtga tgaagcatcc ttgttcgccg gcgatcttta tcgtatgtat tcaaggtatt
     3001 tcgaaaaaca agcttggaaa accgaggtca tctccgttaa cgaaggtact gttggtggct
     3061 ataacaaact cgtacttggt gtaagtggtg aaggtgttta tggcaaactc aaattcgaat
     3121 cgggtgcaca tcgtgtacag cgcgtacctg ataccgaagc caaaggcagg gtacatacat
     3181 cagcggctac agtggctgtt ataccggagt acgaagagga tgacatcacc atcaacaaag
     3241 ccgacctgcg gatcgatact ttcagggcaa gtggtgccgg agggcagcac gtcaacaaaa
     3301 ctgaatcggg tgtacgtatt acgcacatac ctacaggcat tgtttcagaa agtacagaag
     3361 accgctcaca acacaaaaac cgcgacatcg ctatcggtcg tcttatgttc gaaataaaag
     3421 aaacacaaag aaaaaaattc gagtcggaac aggctgccaa gcgtaaatcg ctcgtaggct
     3481 caggcgaccg ttcggacaaa atacggactt ataatttccc tcaaaaccgc gtcaccgatc
     3541 accgcattga cctgaccctt tacaagctcg accaggtact tgaaggtgat gtcgatgaaa
     3601 taatagaagc acttgtagtg tatgaaaatg ctacaaaact tatggaaatg gaaggtgtgt
     3661 gagtatagca aattttacca aaaaaaataa cccgacttca aactaattga agccgggtta
     3721 ttcattttga actcgctttg aaccattaat gccctatgtc ttcaatattc ggaatttcga
     3781 cattcttgta gtcatacagt ttattggctt cttcttcctt aaactgttgg acaagattaa
     3841 gtgcttctgt gactacatca ctacagataa cgataaggct tgcttttttg gcgtgttcta
     3901 tagcatacct gatggcttct gattcttttg ggataatggt tacaggtttg ttggcatcat
     3961 gctgatggat accggcaagt agcaaatcaa taatttcgtg gtctttctta ccacgaagat
     4021 gtttgtcttg cctgatgatg agctcgtcaa acatttcggc acagatacga cctatttcca
     4081 tattgtcttc atctcgtctg tcaccgatac ctgctatgat acctacctta ggtgtgttgt
     4141 cgattttgtt gatgaaatgc tgtaaagctc tcagaccggc aggattgtgg gcataatcaa
     4201 gtaccacatc aaaattcttg aaattgaacc agttaagacg tcccggagta gtagaaggtg
     4261 atggaatgaa agattcgagt gctgtactta tgtcttctat cttaaaatta ttgaggaagc
     4321 cgaccagcac cgatggcaga atattctgaa tcataaatcc ggcacggcca ccgtaggtaa
     4381 gcggaatatt gactgctttg gctacacgta ttttccattc gcccttacag atggtaataa
     4441 aaccattttc gtacagtgcc gaaatacctc cgttcttcga atgttctttg atgcgaggat
     4501 tattttcatc catcgaaaag agggctacct tgctttgtac attgttgcgc atgttataca
     4561 cattgtcatc atccgcattc agtatcgaat aaccttccgg tctgacgctt tctactacca
     4621 cgccttttac tcgtgccatt tgctctactg tatttatacc tttgagcccc aggtggtcag
     4681 cagctacatt ggtaacgatg gccatgtcac agctgtggaa gccaaggcca gcccggagga
     4741 ttcctcccct ggcgcactca agcactgcaa aattgacagt aggatctttg aggacgaact
     4801 cagcacttgc agggcctgta cagtcgcccg acatcatgag gtggttttgg atatagacgc
     4861 catccgtagt agtaaagcca accctgtgcc ccatcatctt caccatgtga gctattaatc
     4921 gggtagtagt ggtctttccg tttgttccgg tgacggctac tattggtatt ctggcagtag
     4981 cacccggagg aaagagcata tcaacaacat tggcagccac gttgcgaggt aagccctcgg
     5041 taggagcaag gtgcatcctg aaacccggtg ctgcatttac ctcaagaaca gctccgccgg
     5101 tgtctgtcaa tggttgtgat atatcggagg tcatgatgtc gataccacag atatcaagtc
     5161 ctatcagtct ggctatcctt tcagccataa atacattata tgggtgaaca gcatctaata
     5221 catcttcggc ggtacctccg gtacttaggt tggccgtatc tttcaggttg aggatttcac
     5281 cgtcaggcaa aacagtatcc agtgtatatc ctttcgaatg aatgatggtc atagtgagat
     5341 cattgaaatc tatctgagtc aaaacttttt cgtgaccgta tcctcttcga gggtcggagt
     5401 tgacgatatc taccagttct ttaatagagt gtttaccgtc gcctatgaca cgggcaggcg
     5461 ttcgtttggc tgctgctact agtttgtagt ctatgaccag aagtctgtaa tcttcgccgg
     5521 ttacaaattt ttcgacgatg acagagttgc tgacacgttt ggcggcttcg aatgcagtaa
     5581 ctgcttcttc ccaactgttg atattgaccg ttatgccacg accgtgattg ccattgatgg
     5641 gttttatcac cagcggatat tttacataac tcacggcatc tttcagacct gcctcagttt
     5701 tgatgatttc gccacgcggc acaggtactt ctgctgcctc gagcaaggtt ttggtatctt
     5761 ctttatcaca ggctatttca acggcgatgc tgctggtctg gctggtgaca gtggcctgta
     5821 tgcgtttctg attggcacca taaccaagct ggcaaaggga atatttattg agccgtatcc
     5881 acggaatgcc tcttgaaatg gcttcttgta cgatagaatc ggtacttggg ccaagtctgt
     5941 tgcgttcacg aagctctctc atatcctgga tgtcggatgc aaggtcgtat ttttcgccat
     6001 ctacaagtgc ctgagctaac ctgacggctg ccctggctgc aaaacgtcct acttcttctt
     6061 caagataggc aaacaccaca tgatactggc caggcactcc ggtctcacgt gtgcggccaa
     6121 aacctacatc cataccagcg agcgtttgta tctcgagtgc gatgtgttcg attacgtgcc
     6181 ccatccacgt cccttcttct atgcgatggt aaaatccgcc tgcataacct tccgaacagc
     6241 ggtgctcata aagactggga agcaattctt tcagacggtc gagaaaaccc ggtatcttgt
     6301 tagttggtcg gtcttcaaga tcttcaaggt cgagcctcat gacgatgagt ttgtgtcttc
     6361 gaatggacca gtaattaggt ccgcgcatgg cattgatttc tagaattttc attatttcct
     6421 gtttttacac attggttaat ttgtcaaata aaattacaat cttcatttcg aaacggaaaa
     6481 taacaaattt ctctcctagt attcagcaga aaaatgtcag aaaatgaata aaaaaaaaat
     6541 gggcagtatt ttatttgtaa aatatgccca atttaaaatt ttattctgaa aaggaaaata
     6601 tcgttcttaa tgcatcatcc tgaaaaatct ttttccggtc ttgctcatag tcggtgcctg
     6661 cttgttgaga tagtatctgg cagtaagcat aaaatactgt gataatgaca gtgcctgata
     6721 atcttcaatg tatgaagaag taacttctct gttgacggct gtgttttgtt tgagaatatc
     6781 ataagccgat aaactcagtt caaaactgtt gttcggcaaa aaagtcttgg tgagagaagc
     6841 attccagatg gtgaaatttt tggtaaaatc gcctgccaaa ccttgtcttt gtgtgaaatt
     6901 gatattcgtc gaaactctga acctgaatgg taggtcggct gtggtgttaa actcagtatt
     6961 gatgtcggtg tagttattat tgaggcgtgg gttgagtgag tacttggctt cattatacgc
     7021 aaccgaacct cccaaatcaa aatcccaggc atcggtgatg ttgtaattga catacagcct
     7081 gccacttgtt gaccacacat cgccattatt gcgttctttg ttgacgaacg atacatcgcg
     7141 tcgatagttg gtagatgaag acacattcag gtttattttt ctgaatggtc ttccatagtt
     7201 ggcgaagaat gatcccgaat agacaccatc cacgttctct ttattggtaa tttcgacacc
     7261 ggactggtca atgaacacat tagatacaat tttgtcgccg gtatattcac cacggacacc
     7321 aaaactcagg aaggtattgc tcaccatatc aaaaatacgg taacttaacc tcatattatg
     7381 ccggtattct tgcccaagat tagggttacc aagtttgata cgcaatggat ctgacaggtc
     7441 tggcacaggc tggatgtcat caatagccgg ctgtcgtgta gatgttctgt aaaacaacat
     7501 gatagactta ttctttgaca atgtataacg tgcgtgcaat ccgggtaaaa agttggtaaa
     7561 gctcttacta aatggtagat tgtcgcttac gctctcgctt cggagtgagg cacgctgtac
     7621 agaacctgat accgtcaccg acagtttttc tagcttatat tggaggctta tctgtggccg
     7681 gtgggtttcg tatgtattgt taaaatcatt gctcaatgta tcctgaaaaa tatcgtacgc
     7741 acccgtagca ggattatatt caaatgattc gcggtttctt gcattctttt caatattata
     7801 attatacgat agttccatgc tgatggcctt gcttaatggt tcagtataac tcagattaga
     7861 gccaagtcca ttgccgtttt ggtagttgac taccttttga ttcagactgt ctgtcgtata
     7921 gctcaaatca gtaaatatct gagaaacaga ctgattattt gttgtattat tatttgaaga
     7981 atatttcggg cttacactga gcgataatgt tcgtccttcc tgtgcgaact tatgcctgta
     8041 caacaggtcg ccacttattc tgaaattttt tgaagccgca tcaacctttg catttacgtc
     8101 attaagactg gcaccgtcag aatttctttg ttttgacaat gacaagctac tggaattgtt
     8161 gtcagaaaaa gataggtttg gttcgaactt aatggaatta ttcttattga ttgcataatc
     8221 aaatccgagg tttactctgt ggttattgcc ggtcgtgtta gcattacccg aaccatccga
     8281 cagcaaggta ttgccttcaa ggaatgtctg cctgtgcgtc tgactttcgc ttctgttgcc
     8341 ggtgttggca aagaaataac tgccggtaaa ttctacctta ccccatgtgt cgcgaaaatt
     8401 tgtaccaatg gttgtatttc ggttaatacc caggttacta gaaccaagca ggctaaaaat
     8461 gctctgtccg ccaatacttg gcccccgcat gccgggacca cccggaccac caccctgact
     8521 gcccccgctt gcattagaaa acgacattgc atcttcagac gtaaaacccg atttgttggc
     8581 attgttgctc atggccagga cagacaattg tcggccctga ttaaaatcaa ataaagtacc
     8641 tgaagattca taacgttctt cggtaccgac acctgtctct acccttccaa acctggcttt
     8701 ccttttgtcg ggtttaagtg taaaattaat tgaccgctct gtatttccat catcaaaacc
     8761 ggagaatgtt gactggtcag attttcgctc gaacacctga accttatcga tggcatcagc
     8821 cggaatattc ttagttgcca gcttagggtc attgccgaag aaaggtttgc cgtcaatggt
     8881 tattttggtc acctgttcgc cttgattttt cactgtcccg tcgttttcca cctgtacgcc
     8941 gggcaacttt ttcaacagat cttcggccat gtcattcggc cgtgtcttga aagtacttgc
     9001 attgaattcg accgtatcat ttttaacctg aacagccact ttttcaccgg tgacctgtac
     9061 gtctttaagc gtaatacccg atttgttgag ccggatggta gcaggccatg gtgtgtcttt
     9121 tgccaaaaac atagaatacg tgtcataacc aagatatttc atctggatga tgacatctgt
     9181 tttttcggta gcattatggt gaatgacacc gttgtcatca gcctgaatgg cacggatgat
     9241 ggtgctatca accggattga gaaggccgcc gagtacaagt ggcaaaggtt cgcctgtctg
     9301 cgcatcgaga atggtagttt tatcttggtt ttgggcaatg acattcgcag ttacaacagc
     9361 catgaggcaa agtacaatta attttttcat aaaggcaaaa acgttcagac tgaattaagg
     9421 tttaattatt ttgattttgc aggccgtagt ttgcccgacg aatgacttct taccttaagc
     9481 aaatcttctt tggtcacctg ttcgcctttg taaattttgt taaaaacgcc cggattatcg
     9541 agcttctttt caattattcg tgcattgtaa tatacctgtg ggccttcgat cttcaataca
     9601 agacctttgg caggctgtat gccgggtaga ggagagaagt taaaatccag ttccttggtg
     9661 taccagatat cgtattcacc catttcattc acgaccttgg cgttatagca tttcaatcca
     9721 agcatttttt gggttttgcc ggtatctttc cacgattttg gtctgggcag cgcctctacc
     9781 gagtaataca tttcattaga ttcattcagt actgtcagag agataatctc tttatgattt
     9841 ttcatatccg tgaaagtata ttcttcaaat ggcagtttga acttaacgcc cggttggttt
     9901 ttaaacattt tgcctccgcc ttttgtgcgt tcgtaataag cgagcgtatc cttgaaatac
     9961 acaaaacggt cttccgtgat ggtattggac agtttctctg taccttcagc agccttgcga
    10021 ccgggtatgc cagtctccct aacgatgtca tagcgtatgg tagccagctt tgtctgttgg
    10081 gcgaatgtaa tagaagtgac aaagcaaagc aggataagca atttaatctt tttcataaac
    10141 aaaagtattg gttcataatt acggacaaaa ttatggtatt ttacgttcaa agacctgaaa
    10201 tatggctata cgatgtgtta agacaaggtt aatgccggga atcatgcatc atcaaaagca
    10261 gaggaatagt ggctaccgga ttcaaaaatt cagatacgcc ggcgagacga tataattgcc
    10321 caagttgctt catcggccgg caaatggtct gaattgcagt tgtgaattgc tttcatttat
    10381 caaaaagttc ttttaatttt ggattcacaa acagcatttc aggcggtgca actttatttg
    10441 ggttggttgt gaattgcttt catttatcaa aaagttcttt taattttgga ttcacaaacn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng tgaattgctt tcatttatca
    10621 aaaagttctt ttaattttgg attcacaaac agcgtcacag ggttgaaacg tactgtttgt
    10681 aaggctttgg gttctgttgt tcaggaattg aaaaacagaa cccaagctga tttttgttcc
    10741 ggtatttttt tatttttatt tttctgacag ctctaaaaaa gcgtcaattg ctgtggtggt
    10801 ggtgttacag attctttttt agtaccgaaa aataactcca tacagccaaa ctgtttgtcg
    10861 gtgatggtga gcacacctac cttgcccttt tcgggtagta tgctctttac atatttctta
    10921 tgcgtttcgg cattttccat gctttggcaa aaacgaaggt aaatcgagaa ctggaacata
    10981 ttgaagccgc gctccagcaa ttctttacga aacttggctg catcgctccg ctcttttttg
    11041 gtctcagccg gcaggtcaaa taatacgaat atccacatgt ttctataagc attaaatcgc
    11101 aaactatcca ttggcgaggt tatttgaaca cggggaaggg aatttttctg atatcgccgg
    11161 cgaaacaacg attgagtccg gcggtggtca tactcacagc atgaaagagg ggccgtgtaa
    11221 tcttgccgat ggttacatct atctgtggca gttttaataa ttctatcttc tgctcccggg
    11281 tgaggccatc tgatggatgt tgctttatca acccaaacac cagccagtca acataaggtc
    11341 tgtatggctc catgatgtcg tctgccaggc agaaagcatt gtacttgttc ttatgaaaaa
    11401 ggccgagtga cggatgcaga ccggtagcgg caagtgcacg ggctatcacc gaacgaagta
    11461 tggcatagcc ataattgagc tgggcattgg gcgtatcgcg ctcctgcgat cgtataaatt
    11521 cagtaccgta gagttggttc caataatgtc cggctgcctg tgcttcgcag ttggtagtat
    11581 cgccactcag tacctgctct ttgagtacat gcagtcgcct gctatttagg tttagcttat
    11641 cgagcaatat cgcctggttg gctatcttgg cctttacggt ctgctgccaa agttgttttt
    11701 taagcggcat gcttgcctct acctgtgttg tcattacctt ggtctgggta gtattggcat
    11761 caaatggcag aaacataccg ctgggcatat acttgctatt gcaggatatg agtgccacat
    11821 tctcttccat aaatgcctct aatactgcag atgatatggt tatctgcggg ttttcgagca
    11881 ctaccatgcc aaggtccatg atgggtacgg tatgccgggt acgaccttca tccttcagct
    11941 ctactatcag ctggctgttg tgcacactca ggtgtgccgg attgctaaaa tgcaaagtac
    12001 gctttaccat gactaat
//
LOCUS       DAOQQY010000055        11935 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1883_length_11935_cov_6.066330, whole genome shotgun sequence.
ACCESSION   DAOQQY010000055 DAOQQY010000000
VERSION     DAOQQY010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1883_length_11935_cov_6.066330"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..719)
                     /locus_tag="PLE23_04415"
     CDS             complement(291..719)
                     /locus_tag="PLE23_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HMX82540.1"
                     /translation="MAENDNGKSERIVTKMFENDAFSRWLGISILEVLPGKVSLSLIV
                     RKEFTNGFGVAHGGITYSLADSALAFSANGLGRHALSIETSVSHTRPVMEGDILTAHV
                     EELVNSRKLGIYHINIYNQRSELVALFKGTVFKKDAEWDV"
     gene            945..4928
                     /locus_tag="PLE23_04420"
     CDS             945..4928
                     /locus_tag="PLE23_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMX82541.1"
                     /translation="MRFKTKLRALYSMRTSRTIIAISAWLSLFLLFSNISAIAQCPLS
                     CRALVNLSLNDKGTFEVTIYTVMNNPQAPCLLNYEIKIFDKDQKPVDNPVTCAYLGQT
                     LTYTVTDTTTGNWCWGNLFVEDKLNPALFCVDVTIQCNDDDSPESIGLVFANDNCDDD
                     PEILSQGEIRTVTDCDNPNGILYTITRTWIAQDTSGNFSAPCTQHIYVRKPNLSQVVF
                     PANLTKANGTDLDCADPDISPDSLGFPTLFGKPLELACQIKSTFTDSIVPQCEGSVNI
                     LRTWFVIDCCNNNFIKHVQIIEKNDKTPPVAICAPGFTLLTEVGKCYRTVTLPKPLIT
                     DNCSDSIKVTIFGSGLGQKGYGPYELAPGLYEITYQVCDGCNNCSYCQLEFEILDKET
                     PTVICNTQLTLPLDTSGNVILTVADLENGTTDNCCLDTLQIKLMGQPDSLLSQQIAFN
                     CSDVNQSFLVILKAEDCHGNTNFCMSSVTIVDTIPPEIICPQDLTVHCNETIDPDSLG
                     VAIAFDNCAIDTIFFQDSTVLTPCNTGNIFRKWTAVDTFSNSSFCIQTISIIDTTDAL
                     ITFPGDVTISCDLNPDTTLTGSPAAVDDCSIFEISFSDSFVNPPVGCNFIVRTWMVKD
                     ICKNTTVPSIQRITLNDVKPPVWLNSAGSLDMTISCDKVFSAPVPTAIDDCTIDNVVI
                     HTLTSNIECKNKYQRTITYRAFDGCGNASVPFFVYVTVRDDLKPIFSFCPKDTIIDAA
                     PNQCGRNINFAPAVATDNCGDLVIVNDSPNTNPNGADISGFYPVGTTIVTIMAIDDCN
                     NRDTCVVNITVRDVTPPFAECAGASVNLIGYINPSDSLYIITPQVIKNNWDYYDLCSS
                     VTVDISPDTFDCNVAGPPPKPFAVTITDAAGNSTVCNGNVLVLDSLKICGDMFADGNI
                     LSGKLSTVGHKPIGGVGVNVEMAGGNQNILSDQDGNFILKKIPEGMSLNIQPQKTDEY
                     LNGVDVLDAILLTRHLLGKKYFTDPYSYLAADVNDSHTLNVADLGEIKRLIVHSQQAF
                     SSNKSWKFLDAKCKNELLEQPLSGKLPESIYLDKLSGSSYFNDFISIKLGDVNMSSKA
                     YGIKSNDTETRTTAPAVLVLPDILLKKDIPTKVAIVLNNAHEINGLQFGMKINDRANT
                     SIIPTPETSIGEEGLHYDKTNNTIYAVYVRDQGIEPKSLSMALLNITIQVDEDIPLRK
                     LISLLPENETTKAMDTHDAECGVELVFSGKSDVPAEQIKIYKNKPNPFKNETIWPVFN
                     PGNAIVAKLTVSGIDGKQLLERELGLESGYQEISISRNDLPASGCYFVKIIGENVNYT
                     GKIVVMD"
     gene            complement(5045..5569)
                     /locus_tag="PLE23_04425"
     CDS             complement(5045..5569)
                     /locus_tag="PLE23_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82542.1"
                     /translation="MTKRSFLVSVLLSIAGAVNAQREIVPTDTLYIKGKIENQICFVL
                     TDLEKFPTVAIEDQIIYNHKGEIKDTIRNLRGIGIKNVLSTVRFLHEKPKNLNEFYLV
                     FIASDGYKVVFSWNEIFNNTAGDHFYIITDINGKKLKDQAQRIIFLATSDLKIGRRYI
                     KGLKTIDVRQVPGL"
     gene            complement(5635..8259)
                     /gene="alaS"
                     /locus_tag="PLE23_04430"
     CDS             complement(5635..8259)
                     /gene="alaS"
                     /locus_tag="PLE23_04430"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMX82543.1"
                     /translation="MRSREIRQNFLEFFASKGHKIVPSAPLVNKNDPTLMFTNAGMNQ
                     FKDYFLGNKVPDARRVADTQKCLRVSGKHNDLEDVGFDGTHHTMFEMLGNWSFGDYFK
                     KEAIEYSWEFLTKVCQIPKDRLYATVFGGDEKEGLEPDRESLEIWLNYLPENRIIYGS
                     KKDNFWEMGDTGPCGPCTEIHADIRPDSERQQVAGETLVNMDHPLVVEIWNNVFIQFN
                     RKTNGELENLPEKHVDTGMGFERLCMVLQQKDKSYDTDVFTPLIDFVESRTGIKYTGS
                     YNREDKSDVATRVVVDHIRAVAFTIADGELPGNSGAGYVIRRILRRAVRYYYSFLGIK
                     EPFMYSLVHILAQEFDGVFPELTAQREFVSKVILEEEKAFLKTLQGGLSRLDTLDLKD
                     GQIDGKTAFELYDTYGFPIDLTKLIALEKGWTIDETGFQAALGEQKARSRADAQKEVG
                     DWVELLNHPDCEFVGYDTCVVNQAKITKYRTSASKGTEVFHIVLDRTPFYGESGGQVG
                     DKGVLISGSETIHIIDTKKENDLIIHITEALPSDVNASVVASVNENLRNATSCNHSAV
                     HLMHAALHRIVGKHALQKGQDVNDQRLRFDFSHFQKLTDEELNAIEAMVNDKIRDNIV
                     LEEHRNMPIEDARNLGAMMLFGEKYGEFVRVVSFDQSFSTELCGGTHVSQTGNIGLFK
                     IVSESAVAAGVRRIEAVTSAKAQEMVDAQIKILDDIKELLRNPASPVKAVHDMIEENK
                     NFRKEIEQLQTKLAAFLKSELKSGFAQGDGFSYLVSEVNLSDAKAVKDLAYQLEKETG
                     NTVIVFGSVNEGKPLITVIVSENLTARFHAGQMVKTLAANIKGGGGGQPFFATAGGSD
                     ASGLSNALRQAKELLTGA"
     gene            8527..9510
                     /locus_tag="PLE23_04435"
     CDS             8527..9510
                     /locus_tag="PLE23_04435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMX82544.1"
                     /translation="MAKEKFVYNKHTLRFEIVQLSLKTYLLRVFGFVSAVVVASVLFL
                     SLAFSYFPSPKEKMLERELDQMAEKYEQINKQMATMDKVLGNIQERDANVHRMMFGMD
                     PIDKNQWNGGTGGHSKFAGFSRLGNSYEILANTEDRVKKLERQLVMQSKSLDTIMNLA
                     IKKENLYSSLPIITPISSDKYRQRLGSLSGFGYRLHPILKVRRMHTGIDFPAPRGTSI
                     QVTGDGVVSKIEPYSSGYGKNVTINHGYGYQSLYAHLESINVKVGQKVKRGQKIGTVG
                     ETGRATAPHVHYEVLVKGQRVNPIQFCLEGISSEEYKELVEMSQNSNVSYD"
     gene            9520..9864
                     /locus_tag="PLE23_04440"
     CDS             9520..9864
                     /locus_tag="PLE23_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HMX82545.1"
                     /translation="MSILDKKLYDEPKRYYSIGEVASMFEVSQSLLRFWETEFPALKL
                     SKNRKGDRLYTEKNIIQLRLIYHLVKEKGFTLEGARHEINALRNREDQKKEIINQLQQ
                     IHKDLTNLLKEL"
     gene            complement(9882..10496)
                     /locus_tag="PLE23_04445"
     CDS             complement(9882..10496)
                     /locus_tag="PLE23_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX82546.1"
                     /translation="MDIILNGLTKKYKQDTIFRDFTFTIKAGDRLGISGPNGSGKSTL
                     LKILSGQVSPTKGERTYLDENGKKIEEELVFGKISYAAPYIELIEEYTLPELLRFYAN
                     FRQPYLPNLQELAELSGLKFKNRFIKEYSSGMKQRVKLLLALCFQSEVVLLDEPSTNL
                     DAEGVAWTNSLITEFLQSRTIIIASNEVRDFEAVPKVLDIVTYK"
     gene            complement(10502..11302)
                     /gene="lpxA"
                     /locus_tag="PLE23_04450"
     CDS             complement(10502..11302)
                     /gene="lpxA"
                     /locus_tag="PLE23_04450"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMX82547.1"
                     /translation="MNTINNLSIIDPKAQIGKNVTISPYAIISGDVVIGDNCWIGPFV
                     SVQDGVRMGKNCKIFQGAIVGSIPQDLKFEGEESVLEIGDNTIIREYCTLNRGTKAGY
                     RTAIGSNCLLMAYVHVAHDCVVGNNVILANNVTLAGHITIGDYAVLGGLTAVHQFVRI
                     GAHAMIGGGSLVSKDVPPYIKASRNPLSYAGINSVGLSRRGFKPEQQQIITDIYRIIF
                     VKGGVISESLKQIETEFNDSAERNTIIEFINHSERGLLKGLRNEVIEE"
     gene            complement(11305..>11935)
                     /gene="fabZ"
                     /locus_tag="PLE23_04455"
     CDS             complement(11305..>11935)
                     /gene="fabZ"
                     /locus_tag="PLE23_04455"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HMX82548.1"
                     /translation="NEPARHKLLDVIGDLSLVGKPIKGKIVATKPGHTSNVQFARLLK
                     KAYNEQRKQQSVPSYDPNAEPILNVMQIAEMLPHRFPFLLVDKVISMSKTQVVGIKNV
                     TMNEQFFTGHFPDNPVMPGVLQMEALAQTGGILALSLQEDKGQWDTYFLKIDNCKFKG
                     KVLPGDTLVLKMELLEPIRRGIVRMQGTAYVGNRIVSEAELVAQIVKRS"
BASE COUNT         3542 a         2454 c         2353 g         3586 t
ORIGIN      
        1 aattgtcttt gtgttattca tacatctctg tataaataac tcgtttctta gggtcaacat
       61 aacgtttatt tcctctctat atgtcaatga actttgctaa tctatttttg tacactgttg
      121 tacaattttc aaaagcggac acaaagataa caaccaatta ttcagaaaac aaaatttatt
      181 ttcaaaaaaa attaaaataa attaaaaaaa atcacaaaaa ttacacaaat atctgaatat
      241 caattaatta aaattaacat atttttttaa aaaaatatgt ctttttttta tcacacatcc
      301 cattcggcat ctttcttaaa gacggtccct ttaaaaagag ctaccagctc tgaacgctgg
      361 ttgtatatat taatatgata aataccaagc ttcctgctat tgacaagttc ttctacatgt
      421 gctgtaagaa tatcaccttc catcaccgga cgggtatgcg aaactgatgt ctctatagat
      481 aatgcatgac gaccaagacc atttgccgaa aaagctaatg cactatcagc taatgaatag
      541 gtgatgcctc cgtgggctac accaaatccg ttcgtaaatt cttttctgac tataagacta
      601 aggctcacct tccctggcag gacttcgaga atgctaatgc caagccacct actgaacgca
      661 tcattctcaa acattttcgt gacgattctt tctgatttac cattgtcatt ctctgccatt
      721 gcatatatat tatagtgtaa tgccgtgaag ataataaaat gctttgaaat atccacatta
      781 attagatact tgaatcagta aatatttaat aaatacaagt tttggcaaaa tatttgatat
      841 tatcataatg tgtttgaatg agggaacaag attagttcaa acacaggacg aattgaaccg
      901 gtatttgcgt ggcaatcaac gaacatccat aaaccggaca tatcatgaga tttaaaacca
      961 agctgcgtgc tttatacagt atgcgtactt ctagaactat cattgcaatt tctgcatggt
     1021 tatcactgtt tctgctattt tcgaatatat cagccattgc ccaatgtcct ttgtcgtgca
     1081 gagcattggt caacctcagc ctgaatgata aaggaacatt cgaagtaact atctatacag
     1141 ttatgaacaa cccgcaggca ccttgtttgc tcaattatga gataaaaata tttgacaaag
     1201 accaaaagcc tgttgataac cctgtgacct gtgcctatct tgggcaaaca cttacttata
     1261 ctgtgactga tactaccacc ggcaactggt gctggggcaa tctgtttgta gaagataagt
     1321 tgaaccctgc attgttctgt gtagatgtta ccatacaatg caatgatgat gattcgcctg
     1381 aaagcatcgg actggttttc gcaaatgata actgtgatga tgatccggag atattatcac
     1441 aaggtgaaat acgcactgtg acagattgtg acaatccaaa cgggatttta tacaccatta
     1501 caagaacctg gatcgctcaa gataccagtg gaaatttttc ggcgccgtgc actcaacata
     1561 tatatgtacg aaagcctaat ctcagtcagg tagtttttcc tgctaatctt accaaggcca
     1621 atgggacgga tcttgattgt gcggacccgg acatttcacc cgactcactt gggttcccga
     1681 ccttatttgg taagccactt gagcttgcct gtcagattaa atctacattc accgacagta
     1741 tagtgcccca atgtgaaggc tctgtgaata tactgcgtac ctggtttgtg attgattgtt
     1801 gcaataataa ttttattaaa catgtacaga ttattgaaaa aaatgataaa acgccaccag
     1861 tagctatttg tgcgcccgga ttcacattac tgaccgaggt aggcaagtgt taccggacag
     1921 tgaccctacc caaacctttg attacagaca actgctctga ctctatcaaa gtaaccatat
     1981 tcggaagtgg acttggtcaa aagggctatg ggccttacga attggctccg ggactctatg
     2041 aaataaccta ccaggtatgt gacgggtgca acaattgttc gtattgtcag cttgagttcg
     2101 aaattcttga caaagaaacg ccaacagtaa tctgcaatac ccaattaact cttccattag
     2161 atacaagcgg taatgtgata ctgacagtag ctgacctgga gaatggtaca acggataatt
     2221 gctgcctcga cacgcttcag attaaattga tggggcaacc ggattctctt ttgtcacaac
     2281 agatagcttt caactgtagc gatgttaacc aatcatttct ggtgatactc aaagctgagg
     2341 actgccacgg taatacaaac ttctgtatgt cttcggtgac aattgtcgat actattcctc
     2401 cagaaattat ctgccctcaa gacctgactg ttcattgcaa cgaaaccatt gatccggatt
     2461 ccttgggtgt ggccattgct tttgacaatt gtgctattga caccatattt tttcaggata
     2521 gcactgtatt gacaccttgc aacacaggaa atattttcag aaaatggaca gcagttgaca
     2581 cattcagtaa ttcttcattt tgcattcaga ctataagtat cattgacaca accgatgcct
     2641 tgataacatt tccgggcgat gtaacaatat cgtgtgatct gaatccggat acaactttga
     2701 ccggatcacc tgcagctgtg gacgactgta gtatattcga aataagtttt tctgattcgt
     2761 ttgtaaatcc accagtcggg tgcaacttta tagtgcgcac ctggatggtg aaggacattt
     2821 gtaaaaacac aacggtacca tctattcaaa gaataacact caacgacgtc aaaccacctg
     2881 tatggctcaa ctcggcaggc agtctagaca tgaccattag ctgtgacaag gttttttcag
     2941 cacctgtacc tacagcaata gatgattgta caattgataa tgtggttatc cacacgctca
     3001 catccaatat tgaatgcaag aacaaatacc agcggactat tacttatagg gcttttgacg
     3061 gatgtgggaa tgcatcggta ccattctttg tatatgtaac tgtgagagat gatctaaaac
     3121 ctatattcag tttttgcccg aaggatacaa taatagatgc cgcaccaaac caatgtggcc
     3181 ggaatatcaa ttttgcaccg gctgtggcaa cggataattg tggagacctg gttatagtga
     3241 acgattcgcc gaatacgaac ccgaacgggg ccgacatcag cggattttat ccggtaggca
     3301 ctactattgt aaccattatg gcaatagatg actgtaataa ccgtgacact tgtgtggtca
     3361 acattactgt cagagatgtg actccgccgt ttgcggaatg tgcaggtgcg agtgtcaatc
     3421 tgataggcta catcaatccg agtgatagtc tatacatcat cacaccacag gttatcaaga
     3481 acaactggga ttattatgac ctttgttctt ctgtgacagt agatatttca cccgacacat
     3541 ttgattgtaa tgttgcaggg cctcctccaa aaccatttgc agtaacaata acagatgctg
     3601 ctggtaacag taccgtttgt aatggaaatg tacttgtact tgattcactc aaaatatgcg
     3661 gagatatgtt tgcggatggc aacatactct caggaaagct ttcaactgta ggccataaac
     3721 caatcggcgg agttggtgta aatgtggaaa tggccggtgg aaaccaaaat attctctcag
     3781 accaggatgg aaactttata ttgaagaaaa ttccggaagg gatgagcctc aacattcaac
     3841 cccaaaaaac agacgaatat ctgaatggtg tagatgttct ggacgcaata ctccttacaa
     3901 gacatttgct tggaaaaaaa tatttcactg atccttacag ttatctggct gctgatgtga
     3961 atgattcaca cacgctcaat gtagccgatc ttggagaaat aaaacggcta atcgtgcata
     4021 gtcaacaggc attttcgtcg aacaaatcct ggaaatttct ggatgctaaa tgtaaaaacg
     4081 aattgcttga gcaacctctt tcaggaaagt taccagaaag catttatctt gacaagctca
     4141 gtggcagttc atactttaat gatttcatat caataaaact gggtgatgta aacatgagtt
     4201 cgaaggcata cggtataaaa tccaatgata ctgaaaccag aacaacggca ccggctgttc
     4261 ttgtactacc tgacatattg ctcaagaaag acattccgac aaaagtggca atagtactca
     4321 ataatgctca cgaaatcaac ggactccagt ttggaatgaa gatcaatgac cgagcaaaca
     4381 caagcataat accaacacct gaaacatcta tcggtgaaga agggttacat tatgataaaa
     4441 caaacaacac gatatatgca gtatatgttc gggaccaagg cattgagcca aaatcattaa
     4501 gtatggcttt gctgaatatt accatacaag tggatgaaga cattccactg agaaaactca
     4561 tcagtttgct gcctgaaaat gagactacaa aagcgatgga cacacatgat gctgagtgcg
     4621 gagtagaact tgttttttca ggtaaatcgg acgtacctgc cgagcagata aagatttata
     4681 aaaacaaacc aaaccctttc aaaaacgaaa cgatctggcc tgttttcaat cccggtaatg
     4741 ctatcgttgc taagcttact gtaagcggca ttgatggaaa gcaattgctc gaaagagaac
     4801 ttggactgga aagcggctat caggaaataa gcataagtag gaatgatttg ccagcttcag
     4861 gctgttattt tgtaaaaatc ataggtgaga atgtcaatta taccggcaaa attgttgtga
     4921 tggattaagc ctgaaaagaa taaacacttc agttatagta gtggttaagc cataaaggga
     4981 aataactgaa gtgtttttta cttttaccag cttcttcatt tacataattg ctactacaat
     5041 ggttttacaa accaggtact tgtcgtacat ctatggtttt gagtcctttg atataccgtc
     5101 ttccgatctt tagatctgac gtagccaaaa aaattattct ttgggcctgg tctttaagct
     5161 tcttaccatt gatgtcggtg atgatataaa agtggtctcc agctgtatta ttaaatattt
     5221 cattccatga aaacacaact ttgtaaccat cagaagctat gaatactaag taaaattcat
     5281 tgagattctt tggtttttca tgaagaaatc taactgtaga taacacattt ttaataccaa
     5341 tgcctcttaa gttgcggata gtatctttta tttcaccttt gtgattgtaa ataatctgat
     5401 cttcgatggc aacagtcgga aatttttcaa gatctgtcaa tacaaagcag atttggtttt
     5461 ctattttacc tttaatataa agtgtatcag tcggtacaat ttctctttgg gcattgacgg
     5521 caccggctat tgataataat acagaaacta aaaaacttct ttttgtcatt ttatcctatt
     5581 ccaaattagt acaatttaca ctgaaactat cctttttttt ctttttcaat aaaatcatgc
     5641 ccctgtcagc aattcttttg cctgcctaag tgcattactc agaccggagg catcgcttcc
     5701 gcctgcagta gcaaagaaag gctgtccgcc accgccgccc tttatgtttg ctgccagcgt
     5761 cttgaccatt tggcctgcat gaaaccttgc tgtgagattt tcacttacga taaccgttat
     5821 gagtggtttg ccttcattga cagagccaaa cacgatgact gtattgccgg tttctttttc
     5881 aagctgatat gccagatctt tgacggcctt tgcatcagac aaattcacct cagagacgag
     5941 atagctgaag ccgtcaccct gagcaaatcc tgatttcagt tcagatttca ggaatgcagc
     6001 aagtttggtc tgaagttgtt ctatttcctt gcggaaattt ttgttttctt caatcatatc
     6061 atgtacagcc tttaccggtg atgcaggatt tctgagcaat tcttttatgt cgtcgaggat
     6121 tttgatttgt gcatccacca tttcctgagc tttggcagaa gtaacggctt ctattcttct
     6181 gacacctgca gctacagcac tttctgacac aattttgaac aagccgatat tgccggtttg
     6241 tgagacgtgt gtgccaccac aaagttcggt actgaacgat tggtcaaagc ttacgaccct
     6301 gacaaattcg ccatattttt cgccaaacag catcattgca ccaaggttgc gcgcatcttc
     6361 aatcggcatg ttgcggtgtt cttcgagtac tatattatcg cgtattttgt catttaccat
     6421 tgcttctata gcattcagtt cttcgtctgt gagtttttga aaatgagaga agtcgaaacg
     6481 caggcgttga tcattgacat cctgaccttt ttgcagtgca tgtttgccta cgatgcggtg
     6541 tagtgctgcg tgcatcaagt gtacggctga gtgattgcaa gaagtggcat ttcgcagatt
     6601 ttcattaacg gaagctacaa cggatgcatt tacgtcagaa ggtaaagcct cagtaatatg
     6661 gatgatgaga tcgttttctt ttttagtatc aatgatgtgg atggtttcgc taccgctaat
     6721 aagcacacct ttatcgccta cctggccacc gctttcaccg tagaagggtg ttcggtcaag
     6781 gacgatgtga aatacctcag tacctttaga tgcagaggtt cggtatttag taattttggc
     6841 ttggttgacc acacatgtat cgtaacctac gaattcgcag tcagggtggt tgagcagttc
     6901 gacccagtca cctacttcct tttgagcatc agcgcgcgat cgtgcttttt gttcaccaag
     6961 ggcagcctga aaaccggtct cgtcgatagt ccatcctttt tccagggcaa taagtttggt
     7021 aaggtcgatg ggaaaaccgt aggtatcgta aagttcgaag gcagtttttc cgtcaatttg
     7081 accgtctttg aggtcaagcg tatcaagacg gcttagcccg ccctgcagtg ttttgaggaa
     7141 agccttttct tcttcaagaa tgacctttga cacaaattcc ctctgtgcag tcaattcggg
     7201 gaatacgcca tcgaactctt gggcaagaat gtgaaccagg ctgtacataa atggttcctt
     7261 gatgccaagg aaggaataat aataacgtac agctcttcgc aggattcttc taatgacata
     7321 cccggcacct gaattgcctg gcaattcgcc atcggctatg gtaaatgcta cggcacgtat
     7381 gtgatcaacc accacacggg tagcaacgtc cgatttatct tccctattgt aactaccggt
     7441 gtattttata ccggtgcggc tttcgacaaa gtcaatcaaa ggtgtaaata catccgtgtc
     7501 ataacttttg tctttctgct gtaataccat acaaagtcgt tcaaaaccca tacctgtatc
     7561 tacgtgtttt tcaggcaggt tttcgagttc gccattggtt tttctgttga attggatgaa
     7621 tacattattc catatttcta caacaagcgg atggtccata ttgacaagtg tctcacctgc
     7681 tacctgctgt ctttctgagt ccgggcgtat gtctgcgtgt atttcggtac aaggaccaca
     7741 cgggccagta tcacccattt cccaaaaatt atcttttttg cttccgtaga tgattctgtt
     7801 ttcaggtaag taattgagcc aaatttcgag cgattcgcgg tcaggttcga ggccttcctt
     7861 ttcatcgcct ccaaaaactg tagcataaag ccgatctttt ggtatctggc agactttagt
     7921 gaggaattcc caggaatatt cgatggcctc ttttttgaaa taatcgccga acgaccaatt
     7981 gcccaacatt tcgaacatcg tatggtgcgt accgtcaaat cctacatctt cgaggtcatt
     8041 gtgtttgcct gatacacgca ggcatttttg tgtatcagct actcttctgg catccggcac
     8101 tttattgccg aggaaataat ccttaaactg attcatacca gcgttggtaa acatgagggt
     8161 aggatcattc ttattgacca aaggagcaga aggtactatt ttatgtcctt ttgaagcgaa
     8221 gaattctaaa aaattttggc ggatttcacg tgatcgcata gggtttttat taggttttac
     8281 gtattattaa gctgctgcgg ttcaaaaact gtataataaa atgatggttt gaaagtaaag
     8341 tgcaaatatc gttcaatcat tttaaataag cggacaatat ttgatatttt gccttaatac
     8401 tattctttaa aattcgataa atatttggag tttataaaaa aatactttaa ttttacgctc
     8461 caatcgctat gaaaaaacaa taatttacgt ccgacattag tatattgcta attaaacgaa
     8521 cgaattatgg ctaaagaaaa atttgtatat aacaaacata ctctaaggtt cgagatagtt
     8581 cagctttcac taaaaacata ccttttaagg gtatttggct ttgtatctgc ggtagtagtt
     8641 gcttctgtat tatttctgtc attagcattt tcatattttc cgtcacctaa agaaaaaatg
     8701 cttgaaagag agcttgacca aatggctgaa aaatatgaac agattaataa acaaatggct
     8761 accatggata aggtgcttgg caatattcag gagcgcgatg ccaatgtaca ccgcatgatg
     8821 tttggtatgg acccaattga caaaaatcag tggaatggag gtacaggcgg gcattcaaaa
     8881 tttgccggat tttcgcgttt aggtaattcg tatgaaatat tggccaatac cgaagacaga
     8941 gtaaagaaac ttgaaaggca acttgtcatg cagtcaaaat cgttggatac gattatgaac
     9001 cttgccatca agaaggaaaa tctttactcg tcactaccca tcattacacc gattagctca
     9061 gataaatacc gacagcgact cggctctttg tcgggcttcg gataccgact acatccgata
     9121 ttaaaagtac gtcgaatgca taccggcatt gattttcctg caccacgggg aacatccata
     9181 caggtaaccg gtgacggtgt agtatccaag atagaaccat actcaagcgg ttatggcaaa
     9241 aatgtaacca tcaatcatgg ctacggttac caatcactat acgcacacct cgaatcaatc
     9301 aacgtgaaag ttggtcagaa agtaaagcga gggcaaaaga taggtaccgt aggtgagaca
     9361 ggaagagcaa cagcgccaca cgtgcattat gaagtattgg tcaaaggaca gcgtgtaaat
     9421 ccaatccagt tctgtcttga aggtatttca tcagaagaat ataaagaact cgtagagatg
     9481 tctcaaaatt cgaacgtttc ttacgactag tatatacaaa tgagtattct tgataaaaaa
     9541 ctctacgacg aaccaaagcg atattacagc attggcgaag ttgcaagcat gtttgaagtt
     9601 tcacagtcac ttctgcgatt ctgggagact gaatttcctg cacttaagct ttcaaaaaac
     9661 cgaaaaggcg atcgattata taccgaaaaa aatataatac aactaaggtt gatttaccat
     9721 ttggttaaag aaaagggttt tacactcgag ggagcaaggc acgaaataaa tgcacttcga
     9781 aaccgagagg accaaaagaa agaaataatc aatcaacttc aacaaataca caaggatttg
     9841 accaatttac tgaaagaact ctgattaact tcccttacca tctatttata ggtcactata
     9901 tccagtactt tgggcactgc ttcaaaatct ctgacttcgt tggatgcaat aataattgtt
     9961 cttgactgta aaaactcagt tatcaggctg tttgtccatg ctacaccttc agcatccaga
    10021 ttggtggatg gttcgtcgag cagtacaact tctgactgaa aacaaagcgc aagcaataac
    10081 tttactcgct gcttcatgcc tgaagaatat tccttgataa accgattttt gaactttaac
    10141 ccggataatt ctgctaattc ctggaggttg ggcaagtagg gttgtcgaaa attggcatag
    10201 aatcgaagca actcaggtaa agtatattcc tcaataagct caatataggg agcggcatag
    10261 cttattttac caaaaaccaa ttcttcttca attttttttc cattttcgtc caaatatgtt
    10321 cgttctccct ttgtggggct tacctgaccg gagagtattt tcagcaaggt agattttccg
    10381 gaaccattgg gacccgaaat gccaagtcgg tcgccggcct taatggtgaa tgtaaagtca
    10441 cggaatatgg tatcttgttt gtatttcttt gtcaggccgt tcagaatgat atccattgtg
    10501 gttattcttc aatcacttca tttctcagtc cttttaataa gccacgttca gagtgattaa
    10561 taaattcgat gatagtatta cgttcggctg aatcattgaa ttcggtttcg atctgtttta
    10621 ggctttctga tatgacgcca ccttttacga aaataatgcg ataaatatcc gtgataatct
    10681 gttgttgttc gggtttaaat cctctacgac tgaggcctac cgaattaatg cctgcataag
    10741 aaagtggatt gcgagaagct ttgatataag gtggcacatc tttacttacg agcgaacctc
    10801 ccccgatcat tgcatgtgct ccgattctta caaactgatg aactgcagta agaccaccaa
    10861 gtaccgcata atcacctatg gttatatgtc cggcaagtgt aacattattg gccagtatca
    10921 cattattacc tacaacacaa tcatgagcta cgtgcacata agccatcagg aggcaattac
    10981 tgcctatggc agtccggtat ccagcctttg taccacggtt aagcgtacag tattcgcgaa
    11041 tgatagtatt atcgcctatt tccagaaccg actcttcgcc ttcaaactta agatcctgag
    11101 ggatggaacc aactatagca ccttgaaaaa tcttacagtt tttacccatt cgaacaccat
    11161 cctgaacact cacgaatggt cctatccagc aattatctcc tatgacgaca tcaccactga
    11221 tgattgcata cggagagatg gtgacattct tgccaatttg tgccttcggg tctattatac
    11281 ttagattatt tattgtattc attattaact cctcttaact atttgtgcta ccagttcggc
    11341 ttctgataca attctattgc ctacataagc tgttccctgc attcttacaa tacctcttct
    11401 tataggttct aaaagttcca tttttaatac cagtgtatca cccggcaata cttttccttt
    11461 gaacttacag ttgtcaattt tcaggaaata agtatcccac tggcctttgt cttcttgcaa
    11521 tgagagcgcg agaataccac cagtttgtgc gagtgcttcc atctggagta cgcctggcat
    11581 cacaggattg tcgggaaaat gacctgtgaa aaattgctcg ttcatggtca cattcttaat
    11641 acctaccact tgtgttttgc tcattgaaat gactttatcg acaagcaaaa atggaaatcg
    11701 gtgtggcagc atttcggcta tctgcatcac atttaatatt ggctctgcat ttgggtcgta
    11761 ggatggtaca ctttgttgtt tacgttgctc gttgtaggct tttttgagca aacgtgcaaa
    11821 ctgtacattg cttgtatgac cgggttttgt ggccactatc tttcctttaa tcggctttcc
    11881 gaccaaagac aaatcaccta ttacgtcgag aagtttgtgg cgtgcaggtt catta
//
LOCUS       DAOQQY010000056        11677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_1948_length_11677_cov_5.606264, whole genome shotgun sequence.
ACCESSION   DAOQQY010000056 DAOQQY010000000
VERSION     DAOQQY010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11677
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1948_length_11677_cov_5.606264"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLE23_04460"
     CDS             complement(<1..582)
                     /locus_tag="PLE23_04460"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX82549.1"
                     /translation="MSIKYLLSVTCLVFVSTTIFAQKYSESNITLHTESGDLQGTLAV
                     PHTSAKVPLAIIIPGSGPTDRNGNTPIGTNNSLKMLAQTLGDKKIASLRFDKRGIAQS
                     KDAAKDESEMRFEDNAADVSLWIELLRKDERFGKITLIGHSEGSLVALLAAKHADAYI
                     SLAGAGRPIDIILKEQLDNIPEDMKAGAFTVIDS"
     gene            complement(631..1203)
                     /locus_tag="PLE23_04465"
     CDS             complement(631..1203)
                     /locus_tag="PLE23_04465"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMX82550.1"
                     /translation="MNFENDDIEHIADLVQQDAVILYPTDTIWGLGCNALSEKAIQKI
                     ISIKGRPPEKGFVVLVESLEMLKNYVEDVNPKIETLLVHHTRPITVIYPQPKGLSPLA
                     CAPDGSVAVRIVTDEFCKELIWACGLPLISTSANISGEPFPANFGEISSEILSAADYV
                     VKYRQNDRSKAEPSTIVKVTHKKELEFIRK"
     gene            1312..3564
                     /locus_tag="PLE23_04470"
     CDS             1312..3564
                     /locus_tag="PLE23_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX82551.1"
                     /translation="MNFISKVFIISFLLLASGNLLAKHIVGGVMSYQCLGNNNYRFTL
                     KVYRDCDPKAGGAGFDAPATLGVYKCGNTINCTSLTQKSAFKKPNIDFGDKANILPPV
                     YPCLKVPPVVCVEEAIYEFTLNLPLSENSYHVVYQRCCRNNTITNIIDPQDSGATYEI
                     ELTPEAQKVCNNSPVFDNFPPTLICINEPLKFDHSASDADGDQLVYELCAPLLGGGKA
                     QGNNQVNGCNGVSPSPACPPPFDFVNFQIPNFTYDKPLGASSVISIDPVTGQLTVNPK
                     QLGQFVVGICVTEYRNGVKLSTVRRDFQFNVANCEPLVNASLKNDKVLGNQQFVLNIC
                     GENTLKFKNESTPSSNIFSYDWYFEGASPQESKEKDATITFPGYGTYKGTMIINKGTT
                     CADTATIYVNVFPDLKADFSFDYDTCKAEPVKFVDKSYSEAGKIENWYWDFQDGSLSS
                     ETNPAHLFKDAGNYPVKLRVFDLNGCSDSITKNVSYFPVPPVVVVKPSRFVGCIPAKI
                     IFDNQSHPIDNTYNVEWDFGDGTTGKAISPTHTYTEPGVYDVGIEITSPIGCKTGSVF
                     NKLIKIEPSPEAGFSYSPSEPSNINPQVTFTDESKLATSWYWDFGDGRTTYIQNPIHT
                     YQDTGVYTVTQIVKHPSGCLDTLIRILDVKPVVTFFLPNAFSPNGDGLNDVYQGQGIF
                     HGIVDFEMTVWNRWGAIIYTSNDPSAGWDGRVNHEGNFVQNGVYVVKVRYKEPRGKEI
                     QLNGFATVIK"
     gene            3643..4002
                     /gene="rplS"
                     /locus_tag="PLE23_04475"
     CDS             3643..4002
                     /gene="rplS"
                     /locus_tag="PLE23_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMX82552.1"
                     /translation="MDAIQYVHQQLNTKTQNKYPEFRAGDNIIVAYRIVEGDKVRIQD
                     YKGDVIQISGAQQSKSFTVRKISNGVGVERIFPYACPNIDNITVVKKGRVRRAKLFYL
                     RSMIGKKARIKELRIFK"
     gene            4155..4931
                     /gene="surE"
                     /locus_tag="PLE23_04480"
     CDS             4155..4931
                     /gene="surE"
                     /locus_tag="PLE23_04480"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMX82553.1"
                     /translation="MNKPLILVTNDDGITAPGIRKLMEIAKQLGEIVVVAPDSPQSGQ
                     GHAITIEQPLRLKKVHIFDDVSSWECSGTPVDCVKLAKNVLLKDRKIDLCVSGINHGS
                     NSSLNIIYSGTMSAAMEAAVENIPSVGFSLLDYSFDADFDQAEPYIRQIMEYVLQNRF
                     EPYTLLNVNIPKLSKDEIQGVRVCRQAKARWIEDYMEAKDPRGQSYYWLTGKFVNEDE
                     GVDTDVFALENGYISVVPAQYDLTFYEQINNFKQLETINP"
     gene            4936..5247
                     /locus_tag="PLE23_04485"
     CDS             4936..5247
                     /locus_tag="PLE23_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82554.1"
                     /translation="MFLKKNNLLFALAAGIFFPILTAAIVMLILEQIIRLGGGEGSAN
                     PVVRPRTVYLIGICINVLLINYYKKLRYDQSMRGIVIATSILGLIWIIRFGSEIMHQI
                     Q"
     gene            5244..6344
                     /gene="lpxB"
                     /locus_tag="PLE23_04490"
     CDS             5244..6344
                     /gene="lpxB"
                     /locus_tag="PLE23_04490"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMX82555.1"
                     /translation="MKYYIIAGEASGDLHASNLVKALKARDEEAVFRAWGGDMMKDAG
                     VELTKHIRELAFMGFVEVLMNIRTILGNIKLCKKDILQWRPDVLILVDYPGFNLRIAK
                     WASQNNIRVVYYISPQIWAWHESRVHQIKKYVDQMLVILPFEEEFYARYNYPVSFVGH
                     PLLDVILEGENVSDRANDPVKTIALLPGSRRQEIKRILPTMLATTRRFPEYRFAIACA
                     PAIPDDFYDALLDGFPEVGRYRGSSYTLLNEAYAALVTSGTATLETALFGVPQVVCYK
                     GDKLSYLIARRLITIKYISLVNLIMDREVVKELIQDEMNEAMLSEELKKLAQPDVRNR
                     IQSDYILLRKILGNQGASGRAADAIIQNMPGR"
     gene            6390..7052
                     /locus_tag="PLE23_04495"
     CDS             6390..7052
                     /locus_tag="PLE23_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMX82556.1"
                     /translation="MLNENEREIIEKTVQFVKRTLAGAEGGHDWFHTQRVWNNAKLIA
                     GSEDVNPFVVELAALLHDIADAKFHDGNEAIGAETTGRFLKSLNVSEDIVGHVVHIVR
                     NMSFRKQLDGVVFSSPEMLVVQDADRLDAIGAVGIGRAFSFGGFKNRAMYDPDIKPET
                     NITGETYRNSNAPTINHFYEKLLLLKDMMNTETAKKIAANRHLVMERFLDDFLDEWHG
                     LK"
     gene            complement(7123..7614)
                     /locus_tag="PLE23_04500"
     CDS             complement(7123..7614)
                     /locus_tag="PLE23_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HMX82557.1"
                     /translation="MIKEVNGDILLTDSKVIAHCVAPADHFESGLALSLRERYPAMVK
                     DFRHYCYTHSPKAGDIWTWGGVGGMQIVNLMAQEPAESMRSRQHGGKASIVNLDRTLK
                     ALAKLAEEEKFDSIAIPRLATGVGGLEWDEVKPSIEKYLGDIPIPVYVYSTFTKDLKA
                     EEK"
     gene            complement(7770..9170)
                     /locus_tag="PLE23_04505"
     CDS             complement(7770..9170)
                     /locus_tag="PLE23_04505"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HMX82558.1"
                     /translation="MKHFILLALAIFSTLKNLSAQAIGLDTLVLNGSADKYINIVFMG
                     DGYTASEQDKFITDAEKMTNYLFSQSPWSDYKNYFNVFAIKVVSNESGTKHPNTASDC
                     SSSNVPVSDPDTYFSLTFDYNGIHRLVAPKNYPNVVKVLSENFPKYDQAIIVANSPYY
                     GGSGGDFATATTHTSSSDVAAHEIGHSFAKLSDEYYAGDGYAYEKVNMTQETDPAKVK
                     WKNWLGFNEIGIYQHCCGGNSAKWYKPSQKCKMQALGKEFCNVCREATIEKIHALVNP
                     IVSYSPATSVISTEDSLITFHLDKLMKPLSNSLQIDWTLDNQPFYTGTDSVQINQSTL
                     AAGKHTLVVTVVDTNLLQRINNHSSIHFSTVTWTIDKKTTSTQVLSAANKISWSVTPN
                     PATSFIRLETESEDLVLLEVNISSLDGKLNKRILKQNIKGTFSDTFDVSSLQPGLYIL
                     TFDMNGQKFTQKLVIQ"
     gene            complement(9167..9679)
                     /locus_tag="PLE23_04510"
     CDS             complement(9167..9679)
                     /locus_tag="PLE23_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82559.1"
                     /translation="MIQPSIVYRYLLSAILLSVVFGTVSCSLFKPSALHDATIQHPSE
                     EKILFLVFRMTGDTLLHNTRLELLQTTITDGAFKQNKLEQPPSPNYLIIEVAANNKNS
                     FKTFAEHPLVRRAEYDAGNELKSKIIHLSEGDFFVRLPIKGNNNKVKITEILESGRRT
                     VLFNNPILSK"
     gene            complement(9786..9932)
                     /locus_tag="PLE23_04515"
     CDS             complement(9786..9932)
                     /locus_tag="PLE23_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82560.1"
                     /translation="MPLRSAPGRSLPEFASLRGKATKVLQAAHADRLHDIIPAFNPDN
                     ILAI"
     gene            10189..10725
                     /locus_tag="PLE23_04520"
     CDS             10189..10725
                     /locus_tag="PLE23_04520"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HMX82561.1"
                     /translation="MTKTNNFVFWVLHIVAWLIFVGLSVEAGGLLVNFFFSIFKPAVI
                     PNLYQKLDLMQMYEASRPSFYAIYSFILILSILKAWLFYIVIMLMLKMDLTKPFTDFV
                     SERILQISYYTLSIGLLSYMARQITKNIMHRGFVPEQVNQFWSDSQAFVLMGAIIYII
                     ATIFKKGVDLQNEHDLTV"
     gene            10756..10950
                     /locus_tag="PLE23_04525"
     CDS             10756..10950
                     /locus_tag="PLE23_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMX82562.1"
                     /translation="MMAKRKMSLNELSEKVDLTLSNLSILKTGKAKAIRFSTLEAICI
                     VLDCQPGDILEFKDEDIVSK"
     gene            11060..11608
                     /locus_tag="PLE23_04530"
     CDS             11060..11608
                     /locus_tag="PLE23_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX82563.1"
                     /translation="MTNTAFPIIQTERLLLRQIVEADIDHIFKGLSHPDIIRYYGVNY
                     QTREATKEQMDFFADLEHNATGKWWAVCSSGDGTFYGAGGLNSLSKEHKKAEIGFWLL
                     TDFWGQGIMKEAMPLICNYGFDQLGLHRIEGFVESANLNCKNAMAKLDFRHEGTMTDC
                     EIKNGEFISLDIYAKIAIGPNI"
BASE COUNT         3443 a         2270 c         2596 g         3368 t
ORIGIN      
        1 gctatcgatg accgtaaatg cccctgcctt catatcttca ggaatgttgt cgagttgttc
       61 tttgagtatt atatcaatag gtcgtccggc acctgcaagc gatatgtatg catctgcatg
      121 tttagcggcc agtagcgcca ccagagaacc ctcactgtgc ccgataagcg ttattttgcc
      181 gaatctttca tctttgcgga gcaattcaat ccataaagat acgtccgctg cattatcttc
      241 gaatcgcatt tcagactcat ctttagcggc atctttactt tgggctattc cccttttgtc
      301 gaatcgcagt gaggctattt tcttatcgcc aagcgtctgg gccagcattt tgagcgaatt
      361 attggtacct attggtgtat tgccattgcg gtcagtagga cccgaacccg ggatgatgat
      421 agccagtggc actttggcag aagtatgagg gacagccagt gtaccttgca gatcacctga
      481 ttcggtatga agcgttatgt ttgattcgga atacttctgt gcaaatatcg ttgtggatac
      541 aaacaccaga caagtaacgg aaagtagata tttaattgac atgcgtattc taataaagtt
      601 tggtaaaagg aaagtttaca ttaaatacaa ttatttgcgg atgaactcga gttctttttt
      661 atgggtgact ttaacgatag tacttggttc ggctttagaa cggtcattct gtctgtattt
      721 tacgacataa tcggcagcag acaagatttc agagcttatt tcaccaaaat ttgcagggaa
      781 tggttcaccg ctaatgttgg cagaagtaga aatgagtgga agtccacatg cccatatcag
      841 ttctttgcag aattcatctg taacaatacg tactgccact gagccatcgg gtgcacaagc
      901 caaggggctc agacctttcg gttgaggata tataactgtt attgggcgtg tatggtgtac
      961 caacagcgtt tctattttcg ggtttacatc ttcgacataa tttttaagca tttcaaggct
     1021 ttcgaccaat acgacaaagc ctttttcggg tggtctgcct ttgatagaga tgattttttg
     1081 aatagctttt tcgctcagtg cattacatcc gaggccccag atagtatcgg taggatagag
     1141 tatcacagca tcttgttgga cgaggtctgc gatgtgctca atgtcatcat tttcgaaatt
     1201 catataccaa ttgaacgaaa accctttttg agagtttaat ttattttaac aaaactaaga
     1261 tttttaaaag aatttagctt ttatttgtgt tttcttttct taattaaaga tatgaacttc
     1321 atttcaaagg tatttattat atcattttta ttattggcaa gtggaaattt gttagcaaag
     1381 cacatagtag gtggtgtaat gtcgtaccaa tgccttggca ataacaatta cagatttacg
     1441 cttaaggtgt atcgggattg tgaccctaag gcgggcggtg cgggttttga tgcccctgcc
     1501 actcttggtg tatataaatg tggcaatacc atcaattgta caagccttac gcaaaaatct
     1561 gctttcaaaa aaccaaatat tgacttcggt gataaagcaa atatcctgcc ccctgtatat
     1621 ccttgtctca aggtgcctcc ggttgtatgt gtagaagagg caatatatga atttacactt
     1681 aatctacctt taagtgagaa tagctatcat gtcgtttatc aacgatgttg ccgaaataat
     1741 acaataacta atatcattga ccctcaagat agtggagcta catacgaaat agaactgaca
     1801 ccggaagcgc agaaagtgtg taataactcg ccggtgtttg ataattttcc gccaacactt
     1861 atttgtataa atgagccatt aaaatttgac cattctgcct cggatgctga cggtgatcaa
     1921 ttggtgtacg aactgtgtgc acccttgctt ggtggtggca aggcacaagg aaataatcaa
     1981 gttaatggtt gtaatggggt ttctccaagt ccggcatgtc cacctccttt tgattttgtg
     2041 aattttcaga tacccaattt tacgtatgac aaacccctgg gtgcaagttc agtaatatca
     2101 atagatccgg ttacaggaca attgaccgtc aacccaaaac aacttgggca atttgtggta
     2161 ggtatctgtg ttaccgaata caggaatggt gtcaaactga gtaccgttcg acgtgacttt
     2221 cagttcaatg tagccaactg cgagcctttg gtcaatgctt cccttaaaaa tgataaagta
     2281 cttggaaatc agcaatttgt attgaatatt tgtggagaaa atacattgaa atttaaaaat
     2341 gagagtacgc catccagcaa tatttttagt tatgactggt attttgaagg ggcatctccg
     2401 caagaatcga aagagaaaga tgccaccatt acttttcccg gctatggtac atataaaggt
     2461 acaatgatta tcaacaaggg cacaacctgt gcagatacag caacaatata tgtgaatgta
     2521 tttccggatc tgaaagctga ttttagtttt gattatgata catgtaaagc agagccggtc
     2581 aaatttgtgg ataaatcata ttcggaagct ggaaaaatag aaaattggta ctgggatttc
     2641 caggatggct cactcagcag cgaaaccaat ccggcacatt tattcaagga tgcaggaaat
     2701 tatccggtta agttgagggt ctttgaccta aatggttgtt cagacagtat cacaaaaaat
     2761 gtcagttatt tccctgtccc accggtagta gtagtaaaac catcgagatt tgtgggttgt
     2821 attccggcca aaataatatt tgacaaccaa tctcacccaa tagataatac ttataatgtt
     2881 gagtgggatt tcggtgatgg tactaccggt aaggctatta gccccacaca tacttataca
     2941 gaaccgggtg tatatgatgt tggaatagaa ataacatctc cgataggatg caaaaccggt
     3001 tcggtgttta ataaactcat aaagattgaa ccaagcccgg aagcaggatt tagttattcg
     3061 ccatccgaac cgagcaacat caatcctcag gtaacgttta ctgacgaatc gaaactggca
     3121 acgagctggt attgggactt tggtgacgga cgaactactt atattcagaa tcctatacac
     3181 acataccagg acacgggcgt atatacggta actcaaatag taaaacatcc atcgggctgt
     3241 cttgatacct tgataaggat attggatgtt aaacctgtag ttacattctt tcttccaaat
     3301 gcattttcac cgaatggtga cggcttaaac gatgtgtatc agggacaggg tatatttcac
     3361 gggattgtag attttgaaat gaccgtctgg aaccgatggg gtgccattat ctacacttca
     3421 aacgaccctt cagccggatg ggatggcagg gtaaaccacg aagggaactt tgtacagaat
     3481 ggggtgtatg tggtaaaagt gcggtataaa gagccacgtg gaaaggaaat tcaattaaat
     3541 ggatttgcga cagtaataaa ataaataaaa attttgattt atatcaaata gtttttacct
     3601 ttgcacctgc ttataaaaaa ggcctttaaa aatatttaga aaatggatgc aatacaatac
     3661 gtacaccaac agttaaacac aaaaactcaa aacaaatacc ctgagttcag agcaggtgat
     3721 aatatcattg ttgcctacag aatcgtagaa ggggataagg tccgtattca ggactataaa
     3781 ggtgatgtta ttcaaattag cggagctcaa caatctaagt cttttaccgt tcgtaaaatt
     3841 tcgaatggtg tgggtgtaga gaggattttc ccttatgcgt gcccaaatat tgacaatatc
     3901 acagttgtca agaaaggacg agtacgaaga gcgaagctat tctacctgag aagcatgatc
     3961 ggtaagaaag ctcgtatcaa ggaattgaga atttttaaat aaatttttat tcatattgca
     4021 caaaagccac tcttgttcag gggtggcttt tgtcatttgg tcaagtttgt ttttgctcat
     4081 tatcgaatat tcttcagctg tcaccaacag aataggcaaa aaactgctaa ttttgatact
     4141 ttaaaaattt acaaatgaat aaaccactga tacttgtaac caacgatgac ggtataacag
     4201 ctcccggaat cagaaaactt atggaaatag ccaaacaatt gggcgaaata gtagtggttg
     4261 cgcctgacag tccgcagtca ggtcaagggc acgccatcac catcgaacaa ccgttgcggc
     4321 ttaagaaagt gcatattttt gacgatgtaa gttcatggga atgttcgggt acaccggtcg
     4381 attgtgtaaa actggccaag aacgtactac ttaaagaccg aaaaatagac ctgtgcgtat
     4441 ccggcataaa ccatgggtcg aacagctcgc tcaatattat ttactccggg actatgtcgg
     4501 ctgcaatgga ggcggctgtt gagaatatac cgtccgtagg gttctcattg cttgattact
     4561 cgtttgatgc cgattttgat caggcagagc cttacatccg acaaataatg gaatatgtat
     4621 tgcaaaacag attcgaacca tatactttac tgaatgtaaa cattcctaaa cttagcaaag
     4681 acgaaataca aggtgtacgt gtgtgcagac aagcgaaggc aaggtggata gaagactaca
     4741 tggaggccaa agacccaaga ggccaaagct actactggct caccggcaaa ttcgtcaatg
     4801 aagatgaagg cgttgataca gatgtatttg ctcttgagaa cggttacata tcggtagtgc
     4861 cggcacaata tgacctaaca ttctacgagc agatcaacaa ttttaaacaa ctggaaacca
     4921 tcaatccgta aacttatgtt tctcaaaaag aataatcttt tgtttgcact ggcggcaggc
     4981 attttttttc cgatattgac agcggctatc gttatgctca tccttgaaca aatcatacgt
     5041 cttggtggcg gtgaaggcag cgccaaccct gttgttcgtc cgcgtactgt gtatcttata
     5101 ggtatttgta taaatgttct gcttattaat tattacaaaa agctcagata tgaccagtcg
     5161 atgcggggga ttgtgatagc gacatccatc cttgggctta tctggattat caggtttggt
     5221 agtgaaatca tgcaccagat acaatgaagt actatattat agcaggtgag gcatccggcg
     5281 accttcatgc ttctaatctg gtgaaggcat taaaagccag agatgaagag gctgtgttca
     5341 gagcttgggg cggcgatatg atgaaggatg ccggtgttga gctgaccaag cacatacgcg
     5401 aactcgcctt catgggtttt gtagaagtat tgatgaatat ccgcaccatt ctgggcaata
     5461 taaagttgtg taagaaagac atcctgcaat ggaggcctga tgtactcatc cttgtagatt
     5521 atccggggtt caatctccgg attgctaagt gggccagcca aaataatatc cgtgtagtgt
     5581 attacatttc acctcagata tgggcttggc atgagtcgag ggtacatcaa ataaagaaat
     5641 atgtcgatca aatgttggtc atactgccgt tcgaagaaga attttatgcc agatacaatt
     5701 acccggtcag ttttgtcggg catccactac ttgatgtcat cttagagggt gaaaatgtat
     5761 cggacagagc gaacgatcct gtaaaaacaa ttgccttact accaggtagt cgcaggcaag
     5821 agatcaaacg gatattgcct acaatgcttg ccactacaag gagatttccg gagtaccgct
     5881 ttgctatagc ttgtgcaccc gccattccgg atgattttta tgatgcacta ttggatggtt
     5941 tccctgaggt tggtcgatat cgaggtagtt catacacatt gctgaacgaa gcttatgctg
     6001 ccttggtaac ttccggtact gccacacttg aaacggcact attcggggtg ccacaagtgg
     6061 tatgctataa aggagacaaa ctctcgtacc tcattgcccg caggcttata acaataaaat
     6121 acatctccct tgtcaacctg atcatggaca gagaagttgt aaaagaactc atacaagatg
     6181 aaatgaacga agcaatgcta agcgaagaat tgaagaaact ggctcagcct gatgttcgga
     6241 acagaataca atctgattat attctgctgc gaaaaatact tggaaaccag ggtgcatcgg
     6301 gtagggcagc tgatgctatc atacagaata tgcccggcag ataatcatca ttctatttca
     6361 atatccaata tcttaacaag gcactgttta tgctaaacga gaatgaacgg gaaataatcg
     6421 aaaaaactgt acagtttgtc aagcgaaccc ttgccggagc agaaggcgga cacgattggt
     6481 tccatacgca aagagtgtgg aacaatgcga agttgatagc cggatcagaa gatgtaaacc
     6541 cattcgtagt agagcttgct gctttactac acgatattgc agatgcaaag ttccatgatg
     6601 gaaatgaggc aatcggggct gaaactaccg gccgatttct taaaagtctg aatgtgtcgg
     6661 aggatattgt aggacatgtc gttcatatcg ttaggaatat gtcgttcagg aaacaactgg
     6721 atggtgtggt gttttcttca cctgaaatgc ttgtcgtgca agatgctgac cgactggatg
     6781 ctatcggagc agttggtatt gggcgggcat ttagttttgg aggtttcaaa aaccgagcaa
     6841 tgtatgatcc tgacatcaaa ccagagacca acatcacagg cgaaacatac aggaacagta
     6901 atgctccgac tatcaatcat ttttacgaaa aactgctatt gctaaaagat atgatgaaca
     6961 cagagacagc aaagaagata gctgctaaca gacacctggt gatggaacga tttttagatg
     7021 attttttgga cgaatggcat ggccttaaat aaacccatag aaaaagtacc ggtagcagat
     7081 gccaaccggt acccatcttt caatcttcta aaccaataat cattattttt cttctgcctt
     7141 caggtctttg gtaaaagtgc tgtaaacata taccggaatc ggtatgtcac ccagatattt
     7201 ttctattgac ggctttactt cgtcccactc aaggccacca acaccggtag caagcctcgg
     7261 aatggcaatg ctgtcaaatt tttcttcctc tgctagtttt gcaagggctt tcaatgttct
     7321 gtcaaggttg acaatgcttg ccttgccgcc atgttgacgt gaccgcatag attctgcagg
     7381 ctcctgtgcc atcaggttta ctatctgcat gccgcctaca ccgccccatg tccaaatatc
     7441 acctgccttt ggactgtgtg tgtagcaata atgcctgaaa tcctttacca tagccggata
     7501 cctttctctg agactgagtg ccaacccact ctcaaaatga tcggcaggtg caacacaatg
     7561 tgcaattacc ttcgaatccg tcaataaaat gtctccattt acttccttaa tcatattgtt
     7621 gataattttt atttcaaaag tacagacttg tgaataaaaa taccttgacc aaagtcagta
     7681 gcggctatga tttttatgcg ttaaatcata aaatttaaac aagcaaaagc tttggaagta
     7741 tatgactctt gttgtaattc acatacaaat cattgtatta caagcttttg tgtaaatttt
     7801 tgcccgttca tgtcaaacgt taaaatatac aggcccggct gaagagaaga gacgtcgaat
     7861 gtgtcagaaa atgtgccttt gatattttgt ttcaatattc ttttgttcaa cttgccatct
     7921 aatgagctga tatttacttc gagcaacacc aagtcttctg attcggtctc aagccttata
     7981 aaagaagtag ccggattggg agttacactc caggagatct tattggcagc tgaaagcact
     8041 tgtgtggatg tagtcttttt gtcgatggtc caggtgacgg tgctgaagtg tatagacgaa
     8101 tggttattga tgcgttgtag cagattggta tctactactg ttacaacaag ggtgtgcttg
     8161 cctgccgcca acgtactttg gttaatctgc acagaatcgg tgccggtata gaatggctga
     8221 ttgtcaagtg tccagtcgat ttggagtgaa ttgctcagag gtttcatgag tttgtcgagg
     8281 tgaaaagtga tcagcgagtc ttcggtagaa ataacggagg tagcaggcga atagctgacg
     8341 attggattga ccagagcatg aattttttcg atggtagcct cccgacacac attgcaaaat
     8401 tctttaccga gtgcctgcat tttacatttc tgactaggtt tgtaccattt ggcagaattg
     8461 ccgccacaac agtgctggta gatgcctatt tcgttaaagc ccaaccagtt tttccatttt
     8521 accttggccg gatctgtttc ttgcgtcata tttaccttct cgtaagcata tccgtcaccg
     8581 gcgtaatatt catcactcag cttggcgaag gaatgcccga tctcgtgggc tgctacgtca
     8641 gagctgcttg tatgggtagt agcagtagca aagtcgccac cactgccgcc gtaataagga
     8701 gaattggcaa cgatgatagc ctggtcgtat ttggggaaat tttcggagag caccttcact
     8761 acattcggat aattcttagg cgccaccagt ctgtggatac cattgtagtc gaatgtaagg
     8821 ctgaagtagg tgtcggggtc tgaaacgggt acattggatg aagaacaatc agaagcagtg
     8881 ttgggatgtt tggtaccgga ttcgttggat accaccttta ttgcaaacac attgaaatag
     8941 tttttgtagt cggaccaagg cgactgtgag aaaaggtagt tggtcatctt ttcagcgtct
     9001 gtaatgaatt tgtcctgttc ggatgctgta tagccgtcac ccatgaacac gatattgatg
     9061 tacttatccg cagagccgtt tagcaccaat gtgtcgaggc cgatggcttg tgccgataag
     9121 tttttgagcg tcgaaaagat ggctagtgcc aaaagtatga agtgtttcat tttgaaagga
     9181 ttggattgtt aaacagaaca gttcgtctgc ccgattcgag aatttcagtg atttttactt
     9241 tgttgttgtt gcctttgatg ggcaaacgca caaaaaaatc tccttcactt agatggatga
     9301 ttttagactt cagttcgttg cctgcatcgt attcggccct tctgaccaaa ggatgttcgg
     9361 caaaggtttt aaaggaattc ttattattgg cagccacttc aatgatgagg taattaggtg
     9421 aaggtggctg ttcgagtttg ttttgtttga atgcaccatc ggtgatggtc gtttggagta
     9481 gttctagcct tgtgttgtgc agcagcgtgt cgccggtcat acggaaaacc aggaatagta
     9541 ttttttcttc agatgggtgt tggatggtag cgtcatgcaa cgcagatggt ttgaacaaac
     9601 tgcaagaaac agtaccgaag acgacactga gcaggatagc agacaacaaa taccggtaaa
     9661 ctatagatgg ctgtatcaag tgacgaacga attaaatgtg caagtaagga attttttttg
     9721 aaatgtccat gagattttgg tttaatatta ataattgttg ttgtttttgt acgaatcatt
     9781 ttcgattaaa ttgctaatat gttatccggg ttaaacgccg gtataatgtc gtgcagtctg
     9841 tctgcgtgag ctgcctggag gactttggtg gctttgccac ggagcgaagc gaactccgga
     9901 agggagcgac ccggagcgga gcggaggggc acgccctaat gatgtgcaga ataacctgac
     9961 taatgaataa atgagatctg gagagctctg tacctgacag tgtaatgcaa cgcctgagat
    10021 aagttatcga aaaaagcaaa acggcagttg gagcactttt cgccggactg ccgaataaga
    10081 ggtcgatttt tgaataggaa aaaatatttt ttgaattata cataaaaatt attgataaac
    10141 aataaataat tattatcttt gcaatgtaat tcaaaaaatc atacaactat gacaaaaaca
    10201 aacaacttcg tattttgggt actgcatata gtagcatggc tcatttttgt aggcttgtcg
    10261 gtagaagctg gcggcctcct tgtcaatttt ttctttagta tcttcaaacc ggctgtcatc
    10321 cccaatctct accaaaaact tgacctcatg cagatgtatg aagccagcag accgtcattt
    10381 tatgctatct acagcttcat actcatcctt tccatcctca aagcatggct tttttatatt
    10441 gtcatcatgc ttatgctcaa aatggatctt acaaaaccct ttacagattt tgtatccgaa
    10501 aggattctac agatcagtta ttacacactt tctattggct tactgagtta tatggcacgc
    10561 cagataacca aaaatatcat gcaccgtgga tttgtccccg aacaggtcaa ccagttttgg
    10621 tctgacagtc aggcattcgt tttgatgggt gccatcattt acattattgc cactattttt
    10681 aaaaaaggag tggatcttca gaacgaacac gatttaacag tttgacctat gcctattgtt
    10741 gtaaacttag acgtgatgat ggcgaaacgc aaaatgtcac tcaacgaact ttccgaaaaa
    10801 gttgacctta cattatctaa cctgtccatc ctcaagacag gaaaggccaa agcaatccgg
    10861 tttagcacat tagaagctat ctgtatagtt ctggactgcc aaccgggaga tattttggag
    10921 tttaaagatg aggatatcgt ctcaaagtag caatgagaat tttcttgcat cttcctgaca
    10981 aagaatctat atttggcaac gtcaaaaaaa ctacaagtaa tgaagtaaat tatcttttaa
    11041 tgcaagcaaa taagaaaaaa tgacaaacac agcatttcca atcatacaaa ccgaacgact
    11101 cttgctcaga caaattgtcg aagcggatat cgaccacatc ttcaaggggc tttcgcatcc
    11161 cgatattatc aggtattatg gcgtaaacta tcagacaaga gaagccacca aagagcaaat
    11221 ggacttcttt gctgacctgg agcataacgc aacaggtaaa tggtgggcag tttgttcatc
    11281 gggcgacggc actttttacg gtgccggcgg actcaacagt cttagcaaag agcacaaaaa
    11341 ggcagaaatc gggttttggc tcctgaccga cttttggggg caaggaatca tgaaagaagc
    11401 aatgccttta atatgcaatt acggtttcga ccaactcgga cttcacagaa ttgaaggctt
    11461 tgttgaatca gccaatctga attgtaaaaa tgccatggcc aaacttgact tccggcatga
    11521 aggcaccatg acagactgtg aaataaaaaa cggtgagttt ataagtttag acatttatgc
    11581 caagatagct atcggtccga atatataaag ggaatattgg tttcagggcg tgcccctgtc
    11641 gggtcaggcc atccgcttta cttcgtgttc gcttctg
//
LOCUS       DAOQQY010000057        11169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2080_length_11169_cov_6.520605, whole genome shotgun sequence.
ACCESSION   DAOQQY010000057 DAOQQY010000000
VERSION     DAOQQY010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2080_length_11169_cov_6.520605"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLE23_04535"
     CDS             <1..444
                     /locus_tag="PLE23_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82564.1"
                     /translation="HLDTSTPRHLDTSTPRHLGIRIKRLFILMLCFMPALVFSQIYSF
                     QSYFISGVSVLPEGTYANCTFTCTSGSVIRLKAELYSFYSCTFESYSGPDLWEGIYVP
                     NQGSIKTEGTNEATIPTSHLANGLYLVIISQGGSNTVKTWAKSTR"
     gene            513..2273
                     /locus_tag="PLE23_04540"
     CDS             513..2273
                     /locus_tag="PLE23_04540"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX82565.1"
                     /translation="MKHTTLIIIIFLFVISFMNGKLWSQEKYDNNWVFGINNPYIPDI
                     VPGYQIYYFNFDDYDLHIKLHGKKLKNLLEFWVSSTTLSDSEGNLQIATSGHRLFNEK
                     IEIIPNGDSLNFNSLWDTALYYAPRETCLIVPEPGNEENAALVVHTPVKRVGKTFDFA
                     CEAVRITKVRLKNGIADTVLFKNVVIDTGLFEYISMCKHANGRDWWVLYPERYKDVVR
                     RFMITPQGAGPVLTQSIEGAGFGTGSPHHRFSPNGEYYVTMHLDGIKRYDFDRCTGLL
                     SNPIGWKASSIENRFAIFSKSSRYLYLSTDTASKLLQFDMNEPVLKADTVALLDTLYD
                     SPEYKEGLYRFRNMQLAPDGKIYIQGGPHRMSVINDPDKGGKACKLTQWSLELPAYEY
                     GSSNRMPNIRLGPLEGGCDTLGIYKKPLAKFRYVKDTLNKGLVEFIDLSDYDPTQWYW
                     DFGDGGQSSEQRPLHTYKQTGVYNVCLTASNKHGSDTYCRYVDIGALATSDVLSTISC
                     KAFPNPATDYIFISVSEPVYQSLRLKVSDVQGRILIQTRFDLQHGFNTLDISKLASGM
                     YFVELQDCYGMRLNSKFVKQ"
     gene            complement(2726..4042)
                     /locus_tag="PLE23_04545"
     CDS             complement(2726..4042)
                     /locus_tag="PLE23_04545"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMX82566.1"
                     /translation="MNEKQIKDYFENFEVPYQPEHWTLMQQRLEIEELAEYTKNKDDD
                     FDRIISRELSDSEVPYNPAHWQLMEEKLAPQSWQGKIIRYKILEAAVILLLLWTSVSV
                     FEMGGVRPQRDIVQSGLNKTQSTGPKSSNTEQPTHSTINSALQTEGRLYAAAKSTKTV
                     SLPAKSAKAIAMTSYNSIKIPNIEVVTDGTAKDQNVTLARHSVRSSIVMNKYLAPSIV
                     VNKDQNKSFRIGVFGLTAIDQNVAFSEKELNTEENNSYGVVVGGGLSFGWRLKQFEIE
                     TGAAYNSISYIHDENDLSNSLDLKNFTSTNRASQKYLRLPFAVKYLFENNRSYKFYAV
                     AGGNVHLHLNPQPDQALADVGQTDNGGKFSASLSSQEVLRPEENLLSRLYYSASIGLG
                     FEKRLNDRASAFIQPEYYHRLYKPAVIQNKDAINAFAISVGVKAKL"
     gene            complement(4039..4611)
                     /locus_tag="PLE23_04550"
     CDS             complement(4039..4611)
                     /locus_tag="PLE23_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMX82567.1"
                     /translation="MDFVLKLSNIESDLIAACIAKESWAQQKLYEMHYPKLMAVCMRY
                     ASHQDEALDLLHEGFIKIFRNLDKYQTNTSLLSWMKTIVINTAIDHLRKMAKHRTEDI
                     GTVYSLEAGTADVNAMINEKDILKCIQVLTPAYRSVFNLYVIEGYSHKEIGELLSITE
                     STSRSNLVKARTKLKEMLKSSFFDDEFDEK"
     gene            5113..6159
                     /locus_tag="PLE23_04555"
     CDS             5113..6159
                     /locus_tag="PLE23_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMX82568.1"
                     /translation="MKKVLLFVASICLSGLLTAQDIHFSQFYMSPLNLNPALTGVMNC
                     NMRLVGNYRNQYASILKSNAFNTFSVSYDQKIPVGRYDYFGVGGTLWGDKAGSAGFGT
                     VQARLSGSYSKRMSGSRYSSNYLVFGADLGLSQRRINFAKLQWGTQHDGNGGYDPNLN
                     SGETKFDRDNFLFLDMSAGLLWFNVFNKTNSFYLGGAFSHMNRANQSFDTDTYDGLYS
                     KFTIHSGLDYVFPYSRIGLNPGIVAFIQGPSMQINTGTNIKFLLSGNKRTYEAFQFGA
                     WVRLANKIDSGILADALILSTRFDFEQFGIGFSYDLNISSLKNATNHNGAFEFSGIYN
                     ICGPERRGVYCPRF"
     gene            6327..6764
                     /locus_tag="PLE23_04560"
     CDS             6327..6764
                     /locus_tag="PLE23_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMX82569.1"
                     /translation="MFGSKKSTETANISPNSAAVNSLVKGTIVEGTVRAESDIRVDGV
                     ILGDLTCSAKVIIGPTGEIKGEVKCVSAIIDGKFEGKLIVEDLLTVRETASVVGDIRT
                     GKLIVQAGAVFNVNCDMGKNTKKPGGKDPNQRIEQVLKAENVG"
     gene            6754..6984
                     /locus_tag="PLE23_04565"
     CDS             6754..6984
                     /locus_tag="PLE23_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HMX82570.1"
                     /translation="MSDNKPLKSRSSAQVVLQYSGIAFEMLVIILVGWFIGKTADEYF
                     KFSKPVFAIIFILIFTMYSLYRVIKSLEKLNK"
     gene            6988..7377
                     /locus_tag="PLE23_04570"
     CDS             6988..7377
                     /locus_tag="PLE23_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82571.1"
                     /translation="MGFCKFFSSLLIIAALAIGLVTLLQYGGFLPSSAYEISLGSVGL
                     FSVFCLIIYLVAVRLASSANIYQFNNVVLISVLGKLALTMAFLAVFRKIYMPESKFVY
                     VPFLLFYILYTIFETYFLTIIGKQKKS"
     gene            7374..7862
                     /locus_tag="PLE23_04575"
     CDS             7374..7862
                     /locus_tag="PLE23_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HMX82572.1"
                     /translation="MTEDIQIKAIINAVDELTFTQLSANKPDIIQLDENVYQLLLNNK
                     NFTLTLLEKLNNKSYKIKINGNMYQVDLKDQLDMMVEKLGLSSKAAGKINEIKAPMPG
                     LVIDVLVTPGQTIQKGDSLLILEAMKMENVLKAAGDGVIDQICVSKGDAVEKNHIMIT
                     LI"
     gene            complement(7885..10839)
                     /locus_tag="PLE23_04580"
     CDS             complement(7885..10839)
                     /locus_tag="PLE23_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82573.1"
                     /translation="MSFLKKTYQNITSKYNGYFNANVLYQEALIKLNNQHVDNYSRIL
                     DLYPYMAVENPKAVGPDMDKVVEKVATVATLRPASDWVDDCYLMAGKAQYLKQDFESA
                     EETLEFMAKNFSPEGMKERLKGINKQKQLEKERKEREKQKELEQRQKEEERELAKVEK
                     EKAKKQAQKEKANEKKAKEKAKKQAQKDKEKAKKQAAKDKKKGKKTKPATPKSNTTES
                     KPDTEKPTGTKSETPADVKPDTKPDQTVAQKAKEEEKKKNEKPDKYFLKRKPAYQEGL
                     IWLARTYIEEQKFDEALSVMNKLLSNPKTFPHVLAKLNELRAYYFLKQKKYELALEPL
                     QAAVNTSDVKKDKARFSYIIAQIHQTNNRMSEALAAYKQVLRFQPGYEMEFNTHLNIA
                     LNGLRAGNTPLPEAKKYLQKMLKDVKNVDFKDQIYFTLAKLDIESKDFATAIENLKAS
                     LVSNTKNKSQRTESYLLLAELYYNNGNYVSSKSYYDSTLTTMATIDERYTQTKRFSNN
                     LTEIAKNQQIVILQDSLIKISKMSSDDQYKLAKNLKKQEEDARKAAQQSVTGQKKSDP
                     IDILSQIENRTNNRSNSLSGGAKSTFFAYDEKQVKKGQKEFVKLWGQRELADDWRRVK
                     GNSDIQQNNPAVATLSSEDDEKMIKEVLAAVPKTPKEVEEAENKIKTAMLNLGRLYQE
                     QLKDDNKSITQLEELLTRFPNNQHELEAWYLLYLAYNSKGDIEGARAYYDKIIGKYPT
                     STYARVLSDPKYLEEKNAEKTRLDRYYDETYALFSSAKYPEASEKIKQASREFGSDNK
                     YKIKFALLNAMIAGNLEGKTAYIGALKEIIAKYPNTEEEKRAKEIIRILGVESAAPLT
                     KDSTSNVMPYSYTPNDIHYVMVILGAALEKINDIKVEITNYNREYYSFDKLKVTNIFL
                     DTQTPLVVIRQFDKADAAFKYVENAAKNMKGFYKDFTPEQLVVISKENYKAFLINKNL
                     DDYKKYLEQNYR"
BASE COUNT         3078 a         2081 c         2257 g         3753 t
ORIGIN      
        1 cacctcgaca cctcgacacc tcgacacctc gacacctcga cacctcgaca cctcggtatt
       61 agaatcaaaa gattattcat tttaatgctg tgtttcatgc cagcattagt gttttctcag
      121 atatattcct ttcagtcata ttttatctca ggtgtatctg tattgcctga aggtacttat
      181 gcaaattgta ctttcacgtg tacttcagga agtgttattc gtttgaaggc tgaattgtat
      241 tctttttact cttgtacatt tgagagttat tcaggtccgg atttgtggga gggaatttat
      301 gtacctaatc aaggcagcat aaaaacagag ggaacaaatg aagcgacaat acctacaagt
      361 catctagcta atggcttgta tctggtaatc ataagccagg gtggtagcaa cacggtgaag
      421 acctgggcga aaagcactag ataagtcatt gaatcattaa aaaaggtgcg gttactttaa
      481 gaccaaacct aactgtataa aatatgaaca gaatgaaaca cacaacatta attattatta
      541 tatttctgtt tgtcatcagt tttatgaacg ggaaactatg gtcacaggag aaatacgaca
      601 acaactgggt attcggtatc aataatccat atattccgga tatagtacct ggttatcaaa
      661 tctattactt caattttgac gattacgatc tccatattaa gcttcatggg aaaaagctaa
      721 agaatcttct tgaattttgg gtaagctcga ccacgctatc agattccgag ggaaatttgc
      781 aaatagcaac ctctggacat aggttgttca atgaaaagat agaaattata cctaatggag
      841 acagcctgaa ttttaattct ctttgggaca cagcactgta ttatgctcca agggagacct
      901 gcctgattgt acctgagcca gggaatgaag agaatgctgc tctggtggta catacaccag
      961 taaagcgggt gggcaaaacc ttcgattttg catgtgaagc tgtccgtatt accaaagtgc
     1021 gcctgaagaa cggtattgcg gatacggtat tgtttaaaaa tgtggttatc gataccggtt
     1081 tgtttgagta catatctatg tgtaagcacg cgaacggtcg tgactggtgg gtgttgtatc
     1141 ctgaaaggta taaagatgta gtacgccggt ttatgataac accgcagggt gcaggcccag
     1201 tactgactca gtcaatagaa ggggcgggtt ttggcactgg tagcccacat catcgtttta
     1261 gtccaaatgg tgaatattat gttaccatgc atcttgatgg gataaagcgg tatgattttg
     1321 acagatgtac gggcttgctt tctaatccca ttggttggaa agcttcaagt atagagaata
     1381 gattcgcaat tttttcgaag agtagccggt atctttatct ctctacggat actgcctcca
     1441 agctactgca gtttgatatg aacgaaccag tgctgaaggc ggatacagta gcgctgttag
     1501 acacactgta cgattcaccg gagtacaagg aaggcctgta tcggtttcga aacatgcagc
     1561 tagcccctga cgggaaaata tacatacagg gaggccctca ccggatgagt gtaatcaacg
     1621 atccggacaa aggaggcaag gcgtgtaagc ttacccaatg gtcattagag cttcctgcat
     1681 acgaatacgg gtcgagcaac aggatgccga atataagact tggcccctta gaaggtggct
     1741 gtgatacact tggcatctat aagaagcctt tagcgaaatt caggtatgta aaagacacac
     1801 taaacaaagg ccttgtagag tttatagatc tcagtgatta tgatcctacg cagtggtatt
     1861 gggattttgg tgacggtggg cagagtagtg agcaacgtcc gctgcataca tataagcaga
     1921 ccggggtata taatgtgtgc ctgacggcaa gtaataaaca tggaagtgac acctattgtc
     1981 ggtatgtaga tataggtgct ttagcaacca gcgatgtgct gtcaaccata agttgcaagg
     2041 cattcccaaa cccagctact gattatatat ttatttcggt ttcagaacct gtgtaccaaa
     2101 gtttacggct aaaagtatca gacgtacagg gcaggatttt aatccagaca agatttgatc
     2161 tgcaacatgg atttaataca ttggatatat caaagctggc ttcgggtatg tattttgtag
     2221 agttgcaaga ttgttatgga atgagattaa acagtaaatt tgtaaagcag tgagctgaga
     2281 ttcaattgag taggaatttg gacaactaat acttttgtcg ttacaaattg aaattacttt
     2341 ccgttgctac acagtcatgc tgatgtacaa gggcagcccg gcgattactg cggtgaatga
     2401 agctgaatga aaccgaaaac ccaacagtta tatattgtaa ctcagaaaag gtaacagact
     2461 ctttaatgcc aatatttatt atttaaaaac catttcaaaa gctcaaaaag ccttgttaac
     2521 aatgtttttt agcttttgta taaatttttt gtactaatta ttttcgttta cattcctaat
     2581 gtgtaatatg ggtttaacag acgatttata atatgaggta caaaattctc taatatccac
     2641 aaacgcaaat actctaccgg tgacgaagat gtgctctacg agcaggtgaa agcactggcg
     2701 aggtaaagag ccgggtgtta ttatattaca actttgcttt tacaccaacc gatattgcaa
     2761 atgcattgat ggcatctttg ttttggataa cggccggttt gtataacctg tggtaatatt
     2821 caggctggat aaacgcgctg gcacggtcat tcaatctttt ttcgaagcct aatcctatgc
     2881 tggcagaata atataatctg ctcaacaaat tctcctcggg cctgagtacc tcctggctgc
     2941 tcaatgaagc agaaaactta cctccattgt cagtttgccc gacatcagct aaggcctgat
     3001 ctggttgtgg attaaggtga agatggacat taccacctgc tacagcatag aatttatacg
     3061 acctgttatt ctcaaataaa tatttgactg caaatggcag acgaagatat ttttgtgatg
     3121 ctcggttagt agatgtgaaa ttctttaggt caagactgtt ggacagatcg ttttcgtcat
     3181 gtatgtatga gatgctgtta taggctgctc ctgtttctat ttcaaattgt ttgaggcgcc
     3241 agccgaacga aagtccgcca cctactacaa caccgtagct gttattttct tcagtattca
     3301 gttccttttc actaaatgct acattctgat cgatggcagt taaaccaaat acgccaattc
     3361 tgaatgattt attttggtct ttatttacaa cgattgaagg tgccaggtat ttattcataa
     3421 caatagaaga acgtacacta tgacgagcaa gtgttacatt ttggtctttg gctgttccat
     3481 cggtcactac ttcaatattt ggaattttta tggaattata gcttgtcatt gcaatagctt
     3541 ttgcactttt ggcaggaagc gatacagttt ttgttgattt ggcagcagca tataatcggc
     3601 cttcagtctg taatgcactg tttatggtag agtgggtagg ctgttctgtg tttgaagatt
     3661 ttggaccagt actttgagtt ttgttcaaac ctgattgtac aatatctctt tgaggtctga
     3721 cacctcccat ttcaaataca gaaactgaag tccaaagtaa aagaagaatg acagcagctt
     3781 cgagaatttt ataacggatt atttttcctt gccagctctg tggtgccagt ttttcttcca
     3841 ttagctgcca gtgtgccgga ttgtaaggca cctcactatc gcttagctcc cggctgatga
     3901 tcctatcaaa atcatcatcc ttgttctttg tatattctgc aagttcttct atttcgagcc
     3961 tttgctgcat aagtgtccaa tgctcaggct ggtacggaac ttcaaaattt tcaaaataat
     4021 cttttatctg tttttcattc atttttcatc aaattcgtca tcaaaaaagg atgactttag
     4081 catttcttta agtttagtac gagccttcac cagatttgac cgcgaagtac tttctgtgat
     4141 actcaataat tcacctattt ctttatgtga atatccttca ataacgtaca ggttaaatac
     4201 agaccgataa gccggagtta gtacctgtat gcatttcagg atatctttct catttatcat
     4261 cgcattgaca tctgccgtac ctgcttcaag actataaaca gtgccgatat cttcggtgcg
     4321 gtgtttggcc atttttcgca gatgatcgat ggcagtattg ataacaatag tcttcatcca
     4381 cgataacaat gaggtgttcg tctggtattt atcaagattt ctgaaaattt tgataaaacc
     4441 ttcatgcaat aaatcaagtg cctcgtcttg gtgcgatgca tatctcatac acactgccat
     4501 tagttttggg tagtgcatct cgtacaactt ttgctgtgcc caagattcct tggctatgca
     4561 tgctgcaata aggtccgact ctatattact tagcttcaat acaaaatcca ttcttcaaaa
     4621 attaaaagta ggccttttta ttcaaatgtc aaagtttggt tagttgattt ttggaatatt
     4681 ataacgcaat atataccaat acgctgcttt aatctataag tgttataatt tttattattt
     4741 gtactcctat gtttgtacgg acatattttt tttgattttt aataaatgta ctttgaggca
     4801 gcaaaacttc tttataaggt atctgctata agaattgaaa actgtgtaat tgacatcaaa
     4861 tcagtttctt aagactgttg attgttgaat tattttaatc ccttgcggga aatgaatgga
     4921 tataggccgc aaaataaaag cacagtttaa aattttaaac agaatataat tttgaactta
     4981 aaaatagtaa ttgttaaatt tatacttggt aataaaaaaa gtttatatat tgccgattca
     5041 aaactttttt agattcaatt tcgtagaatt gttttgctga ttttttcatt aaatagaata
     5101 ttaaaagacc ttatgaaaaa agttttactg tttgtagcaa gtatatgtct gtccggatta
     5161 ttgaccgcac aagacataca tttttcccag ttttacatgt cgccgcttaa cctcaaccct
     5221 gcattgacgg gtgtgatgaa ttgtaatatg cgtttggtag gtaactaccg taaccaatat
     5281 gcttcaattc tgaagagcaa cgcgttcaat actttttcgg tgtcttacga ccaaaagatt
     5341 cctgtaggcc gttatgatta tttcggcgta ggaggaacac tctggggcga caaggccggt
     5401 tctgccggtt ttggtactgt tcaggccaga ttgagcggta gctacagcaa gagaatgtca
     5461 ggtagcagat acagcagtaa ctatctcgtt tttggtgctg acttaggttt gtcacaacgc
     5521 cgcattaatt ttgcaaaact tcagtggggc acacagcacg acggtaatgg tggttatgac
     5581 cctaacctca actcaggaga aacaaaattt gatcgcgata acttcttgtt tctcgatatg
     5641 agtgctgggt tattgtggtt caatgtattc aacaagacca atagtttcta ccttggtgga
     5701 gcattcagtc acatgaaccg cgccaatcag tcatttgata cggatactta tgatggtctt
     5761 tatagcaaat ttaccataca ttcaggtctt gattatgtat tcccatacag cagaatcgga
     5821 cttaacccgg gtatcgtcgc attcatacaa gggccatcta tgcagatcaa tacaggtacg
     5881 aacattaagt tcttgctttc tggcaataag agaacttatg aagcattcca gtttggtgca
     5941 tgggtacgtc ttgccaataa gattgactct ggtatccttg cagatgcact cattctaagt
     6001 actagatttg acttcgaaca gtttggtata ggcttcagct acgaccttaa catctctagt
     6061 ctcaagaatg ctaccaacca caacggagct ttcgaatttt ctggtattta taatatctgc
     6121 ggacctgaaa gacgaggtgt ttactgtcct agattctaaa tattttgtac acatattaag
     6181 agacaagtcc tgtgatatca cgcacaagac ttgtcttttt tgttaatatt aagattattc
     6241 ctttgatttg tcagcggaat aatgtaaatt tacttcgcaa aggatattat ttatacatat
     6301 taattaataa gataatatat aagcttatgt ttggcagtaa aaaaagtacc gaaacggcaa
     6361 atatttctcc caatagtgct gctgttaact cactggttaa aggtacaatt gtagaaggaa
     6421 ccgtaagggc agaaagtgat ataagggtag atggtgttat tttgggcgac ctcacttgtt
     6481 cggctaaggt tatcatcggt ccaacaggag aaataaaagg agaagtgaag tgtgtgagcg
     6541 ccatcattga tggcaaattt gaaggaaaac taattgtcga agacctgctc actgtcagag
     6601 aaacggcaag tgtcgttggt gatatcagaa caggtaaact catcgtacag gcaggtgctg
     6661 tgtttaatgt aaactgcgat atgggcaaaa atacaaagaa acccggcggc aaagacccaa
     6721 atcaacgcat tgagcaagtt ttgaaggctg agaatgtcgg ataataaacc attgaagtcc
     6781 agatcgtctg cgcaggtagt acttcagtat tctggtattg catttgagat gctggtaatc
     6841 atacttgttg gttggtttat tggtaaaacg gcagatgaat attttaaatt cagtaagcct
     6901 gtgtttgcca tcattttcat attgatattt accatgtact ctttgtaccg tgtaataaaa
     6961 agtctcgaaa agctcaacaa gtgagttatg ggtttttgta aatttttttc atccctgctg
     7021 attatagctg ctcttgctat tggcttagtc accttattac aatatggcgg ttttttgccc
     7081 tcatcagcat acgaaatttc acttggctct gtcggtctat tcagtgtatt ctgtttaatc
     7141 atttatctcg tggcagtgcg ccttgcatct tctgccaata tttatcagtt taataatgtt
     7201 gttctcattt cagtattagg caagctggca ttgacaatgg catttttagc tgtttttagg
     7261 aaaatataca tgcctgagtc aaaatttgta tatgtacctt tcctgttgtt ttatattctt
     7321 tatactatct ttgaaaccta ttttctcacc atcattggca aacagaagaa atcatgacag
     7381 aggatataca gataaaggct atcatcaatg cagtggacga actcactttc actcagctat
     7441 cagcgaacaa acctgatatc atccaattgg acgaaaatgt atatcagtta ttactaaata
     7501 ataaaaattt tacgctcact ttgctcgaga agcttaacaa caaaagctac aagataaaga
     7561 taaatggcaa tatgtaccaa gtcgatctca aagatcagct tgacatgatg gtcgaaaaat
     7621 tggggctgtc gagcaaagca gccggtaaaa taaatgaaat caaggcgccc atgcctggtc
     7681 tcgtcataga tgtactcgta actccgggtc aaaccataca aaaaggtgac agcctgctaa
     7741 tactggaagc catgaaaatg gaaaatgtat taaaagcagc cggcgatggt gtcattgatc
     7801 agatttgtgt atcaaaaggt gacgctgtcg agaagaatca catcatgatc acgttgattt
     7861 aacaagagcc tttacacctt aaaattacct gtagttttgc tccagatact tcttgtaatc
     7921 atcgaggttt ttattgatta gaaaagcttt atagttttct ttagagataa ccacgagctg
     7981 ctcaggtgta aaatctttgt agaagccctt catgtttttg gctgcatttt caacatattt
     8041 gaatgcggca tctgccttgt caaactgcct tatgaccaca agtggcgttt gtgtatccaa
     8101 gaagatattg gtaaccttca acttgtcaaa actataatat tcgcggttgt agttggtgat
     8161 ttcaaccttg atgtcattaa ttttctcgag tgcagcaccc aatataacca tcacataatg
     8221 tatatcattt ggtgtataac tgtagggcat gacattagaa gtgctgtctt tggtcagtgg
     8281 tgcggcagac tccacgccca ggatcctgat gatttctttg gcgcgttttt cttcttcagt
     8341 attcggatac ttggctatta tttctttcaa tgctccgatg taggcggttt ttccttcgag
     8401 gttgccggct atcatggcat ttagcaatgc aaatttgatt ttgtacttgt tatcgctacc
     8461 aaactctctc gatgcttgct tgatcttttc tgatgcttcg gggtatttag cagatgaaaa
     8521 taaagcatag gtttcgtcat aataacggtc aagacgtgtc ttttcagcat ttttctcttc
     8581 cagatatttt gggtctgaca atacacgtgc ataagtagaa gtcggatatt tgccgattat
     8641 tttatcataa taggcacgtg ctccctcaat atcgcctttg ctgttgtagg ccaaatatag
     8701 caaataccac gcttcgagct catgttggtt gttcggaaaa cgagtgagga gttcttcgag
     8761 ttgggttatt gatttgttgt catctttcag ttgttcctga tacagccttc cgaggtttag
     8821 cattgcggtt tttattttgt tttctgcttc ctctacctct ttaggcgttt ttggtaccgc
     8881 tgccaaaacc tctttgatca ttttttcatc gtcttcagag ctgagtgttg ctacggccgg
     8941 attgttttgt tgaatgtcgg aattgccttt taccctgcgc cagtcatctg ccagctctcg
     9001 ttggccccat agttttacga attccttctg acctttcttt acctgttttt catcataagc
     9061 aaagaatgtg cttttcgcgc caccacttag cgaattactt ctgttgttgg tgcggttttc
     9121 gatctggctg agaatatcaa tcggatccga ttttttctga cctgtcaccg attgttgagc
     9181 tgctttacgg gcatcttctt cttgtttttt tagatttttg gccagtttgt actggtcatc
     9241 tgaggacatt tttgatattt ttatcagact atcctgtaat attactattt gctgattttt
     9301 agctatttcg gtgagattgt tactaaatct tttggtctga gtatagcgtt cgtcaatggt
     9361 ggccattgtg gtcagtgtac tgtcatagta cgactttgag ctaacgtaat tgccattatt
     9421 gtagtaaagc tctgccagca acaagtatga ctctgtacgt tgagatttgt tttttgtgtt
     9481 ggatacaagc gaggctttca gattttcgat agcagtcgca aaatcctttg attcgatgtc
     9541 aagcttagca agtgtgaaat aaatctgatc tttaaaatca acatttttta catccttgag
     9601 cattttttgc agatattttt tggcctccgg caatggtgta ttgcccgccc tcaatccatt
     9661 gagtgctatg ttgagatgtg tattgaattc catttcgtag cccggttgga atctgagtac
     9721 ttgcttataa gctgcaagtg cttcgctcat acggttgttg gtttgatgta tctgggcaat
     9781 gatgtaggag aaacgcgctt tatctttttt aacatcagat gtattgacgg ctgcctgtag
     9841 aggttcgagt gccagttcat atttcttttg ttttagaaaa taataagctc ttaactcatt
     9901 gagtttggcc aatacgtgtg ggaaagtctt aggattagat agaagcttgt tcattacaga
     9961 aagcgcttca tcaaattttt gttcttcgat atatgtgcga gccaaccata tcaagccttc
    10021 ctgataagcc ggttttctct tgaggaaata tttatcgggt ttttcatttt ttttcttttc
    10081 ttcttcctta gctttttgag caacagtctg gtcaggttta gtatctggtt tgacatcagc
    10141 aggtgtttcg gacttagttc cggttggttt ttcggtatct ggtttacttt ctgttgtatt
    10201 agattttggt gttgccggct ttgtcttttt cccttttttc ttatcttttg cagcttgttt
    10261 ttttgctttc tctttgtcct tttgtgcttg ttttttagcc ttttccttag cctttttttc
    10321 gtttgctttt tctttctgtg cttgtttttt ggccttttct ttttctactt tagccagttc
    10381 gcgttcttcc tctttttggc gttgttcaag ttctttttgc ttttcacgtt cttttctttc
    10441 cttttcaagt tgtttttgct tgtttatgcc ttttagtctt tctttcatgc cttccgggct
    10501 gaaatttttt gccataaact ccagcgtttc ttcggcactt tcaaaatcct gtttcaggta
    10561 ttgcgctttt ccggccatca gataacaatc gtctacccag tcagatgccg gacgaagtgt
    10621 ggctacagta gctactttct ccactacttt gtccatatcc ggccctactg ctttcggatt
    10681 ttcgacagcc atatacgggt acaaatccag aattcttgaa taattatcta catgctggtt
    10741 gttaagtttg attaatgcct cctggtatag gacattggca ttaaaataac cgttgtattt
    10801 ggaagtgata ttctggtagg tttttttcaa aaaagacaca tcctccttct ttttccttgt
    10861 gacacaagaa cttaaaatca gcaaaaatgc caataaaact aaagaatatc tgtttattaa
    10921 tttcaaacca gctaattttg tatttaaaaa taacggcaaa gatagttaaa acacttagct
    10981 ttatacaatc tgccaccaaa gcttatataa ctgttctatt gaaaaattat tttgtttttt
    11041 tgaaatttta atcaaatgga taatccaata aaactcagtt ggaaacagcg tttgaaggag
    11101 aattacaggc ttgttgtcat gcacaatgag acattcaagg aagtaggctc ctataagctg
    11161 aatctgctc
//
LOCUS       DAOQQY010000058        11095 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2109_length_11095_cov_6.786775, whole genome shotgun sequence.
ACCESSION   DAOQQY010000058 DAOQQY010000000
VERSION     DAOQQY010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11095
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2109_length_11095_cov_6.786775"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="PLE23_04585"
     CDS             complement(<1..352)
                     /locus_tag="PLE23_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMX82574.1"
                     /translation="MMKKTIQLFVGLIVFLTVFDSNPVLAQEVWTLEKCINYARENNI
                     SLKQSMISVGQANINLKANKAQRIPSLNYNTSAGYQWGRTIDYGTNTYVNESTNFNSH
                     SIQAGINLYNGGQGS"
     gene            complement(552..2105)
                     /locus_tag="PLE23_04590"
     CDS             complement(552..2105)
                     /locus_tag="PLE23_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMX82575.1"
                     /translation="MKRRKFLKDTAKAAALPIFLNGIPLTAFSKSAMFNAIDNESDRI
                     LVLVELNGGNDGLNTVIPIEHYSKLSALRPNLLIPESKVLKLNDKTGLHPSMSGMKSL
                     YDNAKLKVVQNVGYPNQNRSHFRSTDIWKSGSGASEYLQTGWLGRYFDKKYPGFPEDY
                     PNAECTDPFAITIGSLVSETCQGSSGNFSMALIDPTSLFTLNEGEDGNPNMNTCYGME
                     LGFLREALLQTNSYIQVIKSANDLGKNKVAYPANNSLADQLKLVAKLISGGLKSKVYI
                     TGISGFDTHASQVEDNDTTTGTHADLLSQVSEAIAAFQADLASQGNEERVIGMTFSEF
                     GRQIQSNFSSGSDHGTAAPMFMFGPCVNPGILGNNPMIPDQVKNQEGVAMEIDFRSVY
                     GTILKDWFKMSDADISEILFAGWQHLPLIQSCTTATKEVPEDPIAAKLYPNPIHNNGF
                     VEFFAENELVRISILDAIGHEVKIVSNQKFSTAYHKVGFEMQGLPPGNYYLRLETKRS
                     SVTKAFVKI"
     gene            complement(2119..3807)
                     /locus_tag="PLE23_04595"
     CDS             complement(2119..3807)
                     /locus_tag="PLE23_04595"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HMX82576.1"
                     /translation="MNRRDLFRSFINKSEYSVKESDFAKSSTTALGLGTGMNPYTGAW
                     TYQQAKHLLNRTMFGPTHEQIVQAVQDGLAGTLSKLFQPLADPAPPINYNVTNDPACP
                     LGQTWVNAVFNPFIPNLDNGRRLSLASWQMGVMLAEGVSIQEKMTLFWHGHFVTETVF
                     DSRVVYNNLKLYRQNALGNFKELTKKVTIDPCMLRYLNGNKNTKQAPNENYARELMEL
                     FTLGKGPIAGPGDYTTFTEKDVEEMAKVLTGWTDFVNENDQNNKVSAIFLNGSHHTGT
                     KTLSHRFNNVQIPNAGNQEYSQLIDIIFQHPEAPRFIARKLYRWFIYYNIDETIEQNI
                     IEPMAQMLVQNNFEIQPVVEALIKSEHFFDPEIAGCMIKSPLDYIIGTLKLFKIVLPD
                     STQYIKQYKVWETLFTFSYALQQMYFFAPNVAGWKAYYQSPSFYRLWINSVTLPLRIR
                     VLEGLTTVGVPINGDTYKVDVIQLAEISSDPSDPDTLIADIANYLYPKELTTAQKVFL
                     KKVLLPNGLPDYIWPLGWNEYKNNPNDPGKKAAVEQLLRGLLYTMCDFSEFQLS"
     gene            3959..5131
                     /locus_tag="PLE23_04600"
     CDS             3959..5131
                     /locus_tag="PLE23_04600"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMX82577.1"
                     /translation="MYPNLSYILHALFGTEPDNAFSIVQTFGLFLVFSFLAAAYFFKK
                     ELRRKADEGLFQPTYVDVSAKQPFAWFDNIINGFILGLIAMKTTHIALNYETFQKDPS
                     GFLLTADGPWLILLVVLGVFTFVRYNAWKKIKHSERPAQRVAVYPHDRVGDITVIAAF
                     SGIFGSKLFSVMEDLPGFFQNPVGSFFSGSGLNIYGGLIVGFLAVYYYIRKHNIEPLH
                     VMDAIAPALIISYGVGRMGCQFSGDGDWGIVNNNPVPSWWILPDSWWASTYPHNVIDD
                     GVSIAGCTWQHCMQLAQPVYPTPIYEIIASIVIFLILWALRKRVKVPGVLFFIYMILN
                     GVERFWIEKIRINDKYSFMGLNMTQAEYIAVLYIIVAIGCIIYFTRKHQSKNGANV"
     gene            complement(5794..7371)
                     /locus_tag="PLE23_04605"
     CDS             complement(5794..7371)
                     /locus_tag="PLE23_04605"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HMX82578.1"
                     /translation="MKNVLLLLCFFISTIVLAQDKMTFQSVVRDNNGKPVNGLIRARI
                     TLLQGSETGTIVFQETHYDVPTNVNGLATFQIGDGSNTQGSLSAIDWSQSPYFIRVEI
                     DPTGGPNFSINTVSQMQSVPYAFYAKNSPAGPKGDKGDKGDKGDIGLDGPKGDKGDKG
                     DIGLDGPKGDKGDKGDKGDIGLDGPKGDKGDKGNKGDKGDKGDKGDPGSTLWTKTGNN
                     IYYNTGTVGIGTSVPNSLYKLHVEGDVRATGALYLGTIEKIYDAGANTIGINSDFIPD
                     ITLLRDVGSSSKKWNDIHIGGQANVKVLYFPTAYSTTQSQSFFYNGDISLTESFVPGT
                     DNFYHLGRSNKRWYDVWSVDGSINTSDARLKTNIQPIKYGLKEIMLLKPVSFIWKNRK
                     IDNPTKLGFLAQDLQKVLNEVVKSKTTIDEHDPNAPLVDTEYLGVMYSDIIPVVVKAI
                     QEQQDIIEDMKNQMKELETLKQDYAEQKKLIETLKAENTAQSETQKSMIEVLNQLNAK
                     VDQLSTSSIEAQQVVKK"
     gene            complement(7376..7867)
                     /locus_tag="PLE23_04610"
     CDS             complement(7376..7867)
                     /locus_tag="PLE23_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82579.1"
                     /translation="MKLKNFSFVFLLLLHLSPIASIAQSGFVATGGNSTDGGQSLSFT
                     IGQIDYLNIVQGNILVANQGIQQPYELGTTSTKGLDIPGLEISLFPNPTLKEFNLLVD
                     FKSNPSLLEYNVFDLLGKKILEGGLEKGLNTVSVTQLPSGIYMVKILTQKNETQTFKL
                     LKY"
     gene            complement(8385..9287)
                     /locus_tag="PLE23_04615"
     CDS             complement(8385..9287)
                     /locus_tag="PLE23_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HMX82580.1"
                     /translation="MTHKNDDLIFQYINGELNPVEHETFKERLLTDIELQDEYNACLQ
                     VMDLLNQAGRNELKTKLAQAGKRQDDRRKLQKRLTYFGIAAVMLALVSFTIWKFIGKT
                     ESVGPPSEVIQRDTIINTPPPQDTVRKKPDTTVRAVPPPMAVVKPAKKSVKKEKQVKT
                     DELFAMHFAPSRELSLNPGLRGPSTMDPVAAFMQAYWDKDYKGAVEYYELIPDDIKQN
                     STIKFEYANCMALQGQYKKAEMLFREVKEDEKSRYASQATYYQALMLLKLKRVNEARN
                     ILKQISADEINPYHQEAAKLMSEL"
     gene            complement(9284..9847)
                     /locus_tag="PLE23_04620"
     CDS             complement(9284..9847)
                     /locus_tag="PLE23_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX82581.1"
                     /translation="MREADELLFGRVKQGDSKAIEQVYLKYRDEFFGWAKTRFMCSFQ
                     DLEDVWQETIIIFFENVKNQRLSVLRSSLSTYLFTIGRNILLNKIRAEQRINLVGNLT
                     DFADPIEDIIMYNFDKSEQEPTLTKYFEALGSKCKELLLGRYYLGKSVDVIRQETGLN
                     SNNVVSASLSRCLNRLKDLIKNNNSNK"
     gene            complement(10410..>11095)
                     /locus_tag="PLE23_04625"
     CDS             complement(10410..>11095)
                     /locus_tag="PLE23_04625"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HMX82582.1"
                     /translation="GRMVDGDELENNLHFRKDTMESYFEKTAKPVSKIQKVLTFLVSP
                     IVKWFLLRQSEPYLAWKTGDINLINRFFKLDAIRVGQGKKAAKIVAFLAMYMLFASVI
                     LHAQAVCNISNIKNNEGQLCFALFENQKQFDRERPVMTFCVTKDEVKEGRLEVKMPDL
                     AGSYGITVMDDTDGNGKMKYKLLRIPAEGFGFSDYIHKGILKPVFDKFKVIIRKNEKT
                     HVNVRMQYY"
BASE COUNT         3072 a         2158 c         2130 g         3735 t
ORIGIN      
        1 tggacccctg cccgccgtta tacagattga tacctgcctg aatactgtgg ctgttgaagt
       61 ttgttgactc atttacatag gtgttggtcc cgtagtcgat ggtacgaccc cattgatagc
      121 ctgcacttgt attgtagttc aaggatggta ttcgttgggc tttattggct ttgaggttaa
      181 tgtttgcctg gcctacggat atcattgact gtttgagtga aatgttgttt tcacgtgcat
      241 aattaataca tttttcaagt gtccaaactt cctgtgctaa gaccggattg gaatcgaaaa
      301 ctgttaaaaa gactatcaga ccgacgaaaa gctgaatggt ttttttcatc attcgaaaaa
      361 tttgagtcaa tattaaatgt attttgacag ttgagaagaa tttttatatt aaaatggaat
      421 ttctttggat gaatggagca aaaacactaa tagatacaac aaaatttgaa tagcaattac
      481 acatcagacc actttaagag ttaagtgatg ctgaagaaat attggatgaa taagaaaggc
      541 ttcgtgccgg attatatttt cacaaaagct tttgttactg aactccgctt tgtctccaag
      601 cgcaaataat aattgcccgg cggtaatccc tgcatttcaa atccgacttt gtggtaagct
      661 gtactgaatt tttgattgct tactattttt acttcatgtc cgatggcatc taatatgctg
      721 atacgcacca attcattttc ggcaaaaaat tcaacaaagc cattattgtg tataggatta
      781 ggataaagtt ttgcagcaat cggatcttca ggaacctctt tggtagcggt tgtacagctc
      841 tgtatcaaag gcagatgttg ccagccggca aaaagaattt cagaaatatc ggcatcactc
      901 attttgaacc aatctttaag gatggttcca tatactgatc tgaaatcaat ctccattgcc
      961 acaccttcct gattcttcac ctggtcagga atcatcggat tattgccgag tatgcccggg
     1021 tttacgcaag gcccaaacat gaacatgggt gctgctgtac cgtggtctga accactgctg
     1081 aagttggatt gtatttgacg gccgaattcg gaaaatgtca tgccgataac acgttcttca
     1141 ttaccttgtg atgcaaggtc ggcctgaaaa gctgcaatgg cttctgatac ctgacttagc
     1201 aaatcggcat gtgtgcctgt ggtagtgtca ttatcttcaa cctgtgaagc atgcgtgtcg
     1261 aatccgctta tgccggtgat atatactttt gatttcaatc caccggagat gagcttggct
     1321 actagtttga gctggtcggc aagagaatta tttgccggat aggctacttt gtttttgcca
     1381 agatcattag cagattttat tacctggatg taagagttgg tctgcagtag tgcctcacgg
     1441 agaaatccaa gttccattcc gtagcaggta ttcatgttag gattgccgtc ttcgccttca
     1501 ttaagagtaa acaaagaggt agggtcaata agagccatag agaaatttcc tgaactgccc
     1561 tggcatgttt ctgacaccag agagcctatt gtaatggcaa acgggtcggt acattcagca
     1621 ttcggataat cttctgggaa acccggatat tttttgtcaa aatatcttcc gagccagccc
     1681 gtttggagat attcggatgc tccagaacct gatttccaga tatcggtaga gcggaagtgc
     1741 gatctgttct ggtttggata accgacgttt tgtactacct tgagttttgc attgtcatac
     1801 aatgatttca tgccgctcat tgatggatgc agtccggttt tgtcgttcag cttcagcact
     1861 tttgattccg ggatgagcag attaggcctc aaagcggata atttgctgta gtgttcaatt
     1921 ggaatgactg tattgagacc atcgttacca ccattgagtt ctacaagtac cagtattctg
     1981 tcactttcgt tgtcaatagc attaaacatg gctgatttgc tgaaggctgt cagtggtatc
     2041 ccgttcagaa aaattggtaa tgcagctgct tttgctgtat cttttaaaaa ctttctcctt
     2101 ttcatatcct tggttggctt aagataattg gaattcagaa aaatcacaca ttgtatagag
     2161 cagacctctc agcaattgtt cgacggcagc ttttttgccg gggtcgttcg ggttgttttt
     2221 atattcattc cagccgagcg gccagatata atcgggcaat ccgtttggca ataatacttt
     2281 tttaagaaaa accttctgcg ccgttgtcaa ttcttttgga tatagatagt tggcaatatc
     2341 agcaatcaat gtgtcagggt cagatgggtc agaagagatt tcggctaatt ggatgacatc
     2401 aactttatat gtgtcgccat tgataggcac acccacggta gtgagccctt cgagtactct
     2461 tattctcaga ggcaaggtga cagagttaat ccaaaggcgg tagaaagatg ggctttgata
     2521 atatgctttc catcctgcta catttggtgc aaaaaagtac atttgctgca aagcgtaaga
     2581 gaatgtaaac agtgtctccc aaaccttata ttgcttgata tattgtgtag aatcgggtaa
     2641 aacgatcttg aacagcttta gcgtgccgat gatgtaatca agcgggcttt taatcataca
     2701 gccggctatc tcaggatcga agaagtgttc gctcttaatc agggcttcta ccaccggttg
     2761 tatctcgaaa ttattttgga cgagcatctg ggccattggc tcaatgatat tctgctctat
     2821 tgtttcatcg atattgtaat agatgaacca tctgtacaat tttcttgcaa taaaccgcgg
     2881 agcttcagga tgttggaaaa tgatatcgat gagctgcgaa tattcctgat tgcctgcatt
     2941 aggtatttgt acgttgttga atcgatgaga gagtgttttg gtaccggtat gatggctacc
     3001 attgaggaat atggccgaaa ccttgttatt ctggtcattc tcattgacaa aatcggtcca
     3061 tccggttaat accttggcca tttcttctac atctttttct gtaaatgtag tataatcgcc
     3121 gggccctgca attggtcctt tgccaagtgt gaacagttcc atcagttcac gggcgtagtt
     3181 ttcgttggga gcctgtttgg tgtttttatt accgttcagg tatcgcaaca tacaagggtc
     3241 gatggtaact ttttttgtca gttctttgaa atttccaagc gcattttgtc tatacagttt
     3301 aagattattg taaacaaccc tcgagtcaaa aacagtctca gtgacaaagt gtccgtgcca
     3361 gaagagggtc attttttctt gtatagatac accttctgcc agcattacac ccatctgcca
     3421 tgaggcgagc gaaagccttc taccattgtc aagatttggg atgaatggat tgaacactgc
     3481 attcacccat gtctgcccca gcggacaggc aggatcattg gtaacattgt agttgatagg
     3541 tggtgccggg tctgccagag gctggaataa tttggaaagt gtacctgcca gaccatcctg
     3601 tacagcctgt actatctgtt cgtgggtagg gccaaacata gtacggttga gcaaatgttt
     3661 tgcttgctgg taagtccatg caccggtgta tggattcatg cctgtaccaa gacccaaagc
     3721 agtagttgag ctttttgcaa aatcactttc tttgacagaa tattcactct tatttatgaa
     3781 acttctgaat aaatctcttc tgttcataat agctttttat gtgaatcaac taatgaaaat
     3841 tcgaataaat gtaatcagga tttttatatt aaaaaataaa aggaataata tttaacatct
     3901 aacgtttagt gttgcattaa aaggttttat taatttagcc acttattcaa taccatatat
     3961 gtacccaaat ttatcctaca tccttcatgc actgtttggt accgagccgg ataatgcttt
     4021 ttccatagtc cagacgttcg gactttttct ggttttttcg tttcttgcag ctgcctattt
     4081 ctttaagaaa gaactcagga gaaaagccga tgaaggtcta tttcagccaa catacgttga
     4141 tgtatctgca aagcagccgt ttgcctggtt tgataacatt atcaacggat ttattttagg
     4201 acttattgct atgaagacca ctcacatagc acttaattat gaaacatttc agaaagatcc
     4261 ttcaggattt ctgcttactg ctgatggccc ttggttaata cttttggttg tattgggtgt
     4321 ttttacattt gtcagataca atgcttggaa aaaaatcaag cattcagaaa ggccggcaca
     4381 acgagtagct gtttatccgc atgaccgggt aggtgacatt acggttatag cggctttttc
     4441 gggcattttt gggtcaaaat tattttcggt tatggaagat ttgcccggtt tcttccagaa
     4501 tcctgtgggt agtttttttt caggtagtgg gctgaatata tatggaggcc tcattgttgg
     4561 ttttctggcg gtctattatt atatccgcaa gcataatatc gagcccctac atgtgatgga
     4621 tgccatagct cctgctctga ttatttcgta cggtgttggt aggatggggt gccagttttc
     4681 aggtgatggt gactggggta ttgtcaacaa taatccggta ccctcctggt ggatattgcc
     4741 cgatagctgg tgggcttcta catatccgca caatgttata gatgatgggg tctctattgc
     4801 cgggtgtacc tggcagcatt gtatgcaatt ggcccaacct gtgtatccga ctcccattta
     4861 cgaaatcatt gcttctattg taatatttct gattttatgg gcattgcgca aaagggtgaa
     4921 agtgccaggt gtacttttct ttatttatat gatactgaat ggtgtagaaa ggttttggat
     4981 agagaaaata cgtatcaatg acaagtattc gtttatgggg ctgaatatga cccaggcaga
     5041 atacattgca gtactttaca ttattgtagc cattggttgt attatatatt ttacaagaaa
     5101 gcatcaaagc aagaatggtg cgaatgtata gttttgtcta aatgaataaa aaatccggat
     5161 gttattcagg catccggatt tttttgtttt aatacgaggg gcaataaaaa ttctttctga
     5221 cttgtttatt ttaggtcagc aaaagtgtca ttacccggtt tatatttttc aatatttaag
     5281 aagttaagaa aggttcagga ttttattaca tgcttcttca aataatggaa gctgatggga
     5341 aaccttttgt atgaaatctt gcgccaaaac aataagattt gctttctcat catcacttct
     5401 tgcagtgcca atgtgtttta ttatcttcct ttttcggtta ccggggataa atgtgtgaca
     5461 cggccaatac tttggtactt acctagataa ttcataagcc tacgaaataa aagatgaccg
     5521 gatttcctta atccaataag gatggacatc ctcgaagaaa ttacatccaa tttccataag
     5581 gaaaaaattg taaggttccc ttacagaaaa accaattatt ttttctcgca aaaatgtcca
     5641 gcaattaaca gataaacaga catttatgct tggaattttc tagtgacatt cattaggaat
     5701 tttaccctat tgaatttttt ggtgcttatt ttttcatatt aaacataaaa ttaaaaggtg
     5761 ccgaagactt tgctccggca ccgggaggaa attttatttt tttactacct gttgggcttc
     5821 aatacttgaa gtggagagtt gatcaacttt tgcattcagt tgatttaata cttcaatcat
     5881 tgatttttga gtttctgact gagctgtatt ttctgcctta agggtttcta tcagtttctt
     5941 ttgctcggca taatcttgtt tcaaagtttc aagctctttc atttgatttt tcatatcttc
     6001 aatgatgtct tgttgttctt ggatggcttt aactactacc ggaataatat ctgagtacat
     6061 gacacctagg tattcagtat caacaagagg tgcatttgga tcatgctcgt caatcgttgt
     6121 tttgctttta acgacttcgt taagaacttt ttgaaggtct tgtgcaagaa atcctaattt
     6181 tgtaggattg tctatctttc tatttttcca aatgaaagat acaggtttta gaagcataat
     6241 ttcttttaaa ccatatttta taggctggat gtttgttttt aagcgggcat cagacgtatt
     6301 aattgagcca tctacactcc atacatcata ccaacgtttg ttggatctac ccaaatgata
     6361 aaagttatca gtacctggga caaatgattc agtaagcgat atgtctccat tataaaagaa
     6421 tgactgcgat tgggtagtag agtaagcagt tgggaaataa agtactttca catttgcctg
     6481 acctccaata tgtatgtcat tccatttttt agatgaagac ccaacatctc ttaaaagagt
     6541 aatatcaggt ataaaatctg agttaatccc tatggtattc gcaccagcat catatatttt
     6601 ttcgattgta cccaaataaa gcgcaccagt agctcgtaca tcgccctcta catgtaattt
     6661 atataaagaa ttaggtaccg atgtacctat acctacagta ccagtattgt aataaatgtt
     6721 attacctgtt ttggtccaaa gagtacttcc agggtcccct ttatcacctt tgtcgccttt
     6781 atccccttta ttccctttat cacctttgtc gccttttggt ccatcaaggc cgatatcgcc
     6841 tttgtcgcct ttgtcgcctt tatcgccttt agggccatca agaccgatat cacctttatc
     6901 gcctttatcg cctttagggc catcaagacc gatatcccct ttatcacctt tgtcgccttt
     6961 atcccctttc ggacccgcgg ggctattttt tgcataaaaa gcataaggca cactttgcat
     7021 ctgtgatact gtattgatac tgaagttcgg acctccggtc ggatcgattt caacccttat
     7081 gaaatacgga ctttggctcc agtcaatagc actcaatgag ccttgggtgt tacttccatc
     7141 acctatctgg aatgtagcaa gaccatttac attcgtaggc acatcataat gtgtctcctg
     7201 aaaaacgatc gtaccggttt ctgacccttg caacagggtg attcttgccc ggatcaaacc
     7261 atttaccggc ttgccattat tatctcttac tactgattgg aaggtcattt tgtcttgagc
     7321 caagacaata gttgaaataa agaaacacaa taataataat acgtttttca taattttaat
     7381 attttagaag tttaaatgtt tgggtttcat ttttttgggt caaaatcttg accatatata
     7441 ttcccgacgg cagttgagtg accgaaactg tgtttaagcc tttctctaat ccaccttcaa
     7501 ggattttctt tccaagtaag tcaaagacat tatattctag taatgaagga ttagatttaa
     7561 aatcaaccag cagattgaat tctttcaggg tagggttggg gaataaagat atttccaaac
     7621 ctggaatatc cagacccttg gttgatgttg tacctaattc ataaggttgt tggatacctt
     7681 ggttggccac cagaatattt ccttgtacaa tattcaggta atcgatctgg ccaatagtaa
     7741 aacttaaaga ttgcccgcca tcagtagaat tgccacctgt ggccacaaag ccggattggg
     7801 caatgctggc gatagggctt agatgcagca gtaataaaaa aacaaaagaa aaatttttaa
     7861 gtttcataat caggagtttt aatcattcat aaaattttca taattccact ttcaatataa
     7921 tacagtattt gccggaacaa tatcaatgca ttcaatttct tatgctttga tgattattca
     7981 ccaaaatcaa ttgtttacta tttgtactac aaaatcaggt tcaaagtatt aaggttggcc
     8041 aacctcactg tttaacatgt tactcattaa tatcatttga tgaaaaatct taacatggat
     8101 ttggaagttt tttaaatcat tcttaaacag tatcaatatt gcgcaaaaac taattgtcct
     8161 tgtaacgtgg gtaataaagg gaaaataaaa gccgatatga atatggtggg tgggataaaa
     8221 ttagaaagat tttcttacaa aggcttatgt aaaatgtctt ttattcattt aaaaagaaaa
     8281 aactatgctt tctttaaata tatatcacaa aatgatgtcg gcttatatta tgcacccaat
     8341 cattctgtac tcgaatgtac ttatcattta aatggtcagc tatctcacag ctctgacatc
     8401 aattttgcgg cctcttggtg atatggattg atttcgtcgg cggatatttg cttcaatata
     8461 ttgcgggctt cgttcactct ttttagcttt aacagcatta gagcctgata gtatgttgcc
     8521 tggctggcat accttgactt ttcgtcttct tttacttcac ggaaaagcat ctctgccttc
     8581 ttgtactgcc cttgtagtgc catacaattg gcatattcaa atttgattgt actgttttgt
     8641 ttaatgtcat ccggaatcaa ttcgtagtat tctactgccc ctttataatc cttatcccag
     8701 taggcttgca taaacgctgc caccggatcc atggtgctgg gtcctcgcaa acccgggttg
     8761 agagaaagtt ctctgcttgg tgcaaaatgc atggcaaata attcgtctgt ttttacctgt
     8821 ttttcctttt tgactgactt tttcgccggc ttgacaactg ccataggggg cggtactgcc
     8881 ctcaccgtgg tgtctggctt tttacgcact gtgtcttgag gcggaggcgt attgatgatt
     8941 gtatctcttt gtattacttc tgagggcggc cctactgact ctgttttacc gatgaacttc
     9001 catattgtaa agctgaccaa agccaacatc actgctgcga ttccgaaata agttaatctt
     9061 ttttgcaact tacgtctgtc atcctgcctt ttccctgctt gcgccagttt ggttttaagc
     9121 tcatttctcc cggcttgatt taacaaatcc attacctgca gacaagcatt gtactcatcc
     9181 tgtagttcaa tatcagtcag caatctttcc ttaaatgttt catgttcaac cggatttaat
     9241 tctccgttga tgtattgaaa aataaggtcg tcattcttat gtgtcatttg tttgagttat
     9301 tgtttttgat caaatccttc agtcgattga gacagcgact aagagatgcg cttacgacat
     9361 tattgctgtt caggccggtt tcctgtcgaa taacatctac cgacttgccc aggtaatatc
     9421 tacctaatag caactctttg cacttactac ccaaagcttc aaaatattta gtcagggtag
     9481 gctcttgttc ggatttgtca aaattataca taataatatc ttcaatggga tcagcaaaat
     9541 ctgttaaatt accaaccaaa ttaattcttt gctctgctct tatcttattc agcaggatat
     9601 tacggcctat ggtgaaaaga taagtactca agctgcttct taataccgaa agtcgctgat
     9661 tcttaacatt ttcaaagaaa ataattatcg tttcttgcca aacatcttct aaatcttgaa
     9721 aactgcacat gaatcttgtt ttcgcccatc cgaaaaattc gtcgcgatac ttaagataga
     9781 cctgctctat tgctttgctg tcaccttgct ttacccttcc aaataataac tcatctgctt
     9841 ctctcatccc atggatttgt gaaagtattt tgcagcaata taactacttt tattttttta
     9901 gtttcacata tttcataaca atatttaaat gattctataa ataatcattt atatcaataa
     9961 attttaaaaa aacatcttac acatttattc tacaatttat cattattaag cgtattataa
    10021 caaatatttg ttgtgtaagc aaatacaaac aaataaaatt caaaattgtt tcagaataat
    10081 aaaatccctg cgacattttg atgccacagg gatcaattca cataaaaaaa tgcattttgc
    10141 tccaggatat tttttaaccc caacacatcc agtgaaacaa aaccagggtt ttcataattt
    10201 gattattaaa tgtttaaaac aattcctaca ttcttacatc ttaatcacct gagcaattaa
    10261 aatcttaaca ccatgcatca attatttttt ctttttttga aattcaatga ttaacgggtc
    10321 atcaggaaca aaataaaact gcctggttgc ttacaaaagg atcctgatat gagctaacat
    10381 cggtgccttt tcaacatatt ttacaaagac taataatact gcatccttac attcacatgt
    10441 gttttctcgt ttttcctgat aatcacctta aatttatcaa aaaccggctt gaggatacct
    10501 ttgtgtatgt aatcagaaaa gccaaatcct tctgccggaa tgcgtagaag cttgtatttc
    10561 atctttccgt ttccatccgt atcatccatc actgttattc catagcttcc tgcgagatct
    10621 ggcattttaa cttccaaccg cccttctttt acttcatctt ttgtaacaca gaaagtcatt
    10681 acaggtcgtt cccggtcaaa ctgtttctga ttttcaaaca gtgcaaaaca cagttgtcct
    10741 tcattattct tgatgttgct gatgttgcac actgcttgag catgcaatat gaccgaagcg
    10801 aataacatgt acatagccag aaaagctaca atttttgcag cctttttgcc ttgcccgact
    10861 cttattgcat ccagcttgaa gaatctgttt atcagattga tgtcacctgt tttccatgcc
    10921 aggtacggtt ctgattgacg gagtagaaac catttgacaa tcgggcttac taggaaggtc
    10981 agaacttttt ggattttgga gactggtttt gctgtctttt cgaagtacga ctccatggta
    11041 tcttttctga aatgaaggtt attctccagt tcatcaccat ctaccatgcg accgc
//
LOCUS       DAOQQY010000059        11078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2114_length_11078_cov_6.971333, whole genome shotgun sequence.
ACCESSION   DAOQQY010000059 DAOQQY010000000
VERSION     DAOQQY010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2114_length_11078_cov_6.971333"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="PLE23_04630"
     CDS             <1..303
                     /locus_tag="PLE23_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82583.1"
                     /translation="DTLIWSYSKENAPFSVKIFENGNPDYGYWFKLNPDKVLYKPGVV
                     YFIQGPGEPPFLDFEGGPITPTTPGVQSYFDYYLNNNILTTGDTLIIGQLNAEYKP"
     gene            complement(379..1968)
                     /locus_tag="PLE23_04635"
     CDS             complement(379..1968)
                     /locus_tag="PLE23_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMX82584.1"
                     /translation="MKRRNFLKTIPVAIGGLTVHAYGASPLLSALSANLTNTDHVLVI
                     VQLSGGNDGLNTVIPLDQYSALSQLRSNVLIEESKALVLSGTGGKTALNPAMTRFAEM
                     WEEGELAIVQGVGYPNFNYSHFRATDIMMTGSDSDQILNSGWAGRYLNYEYPNFPVGY
                     PNATVPHPLGIRIGGNVGLGLQNMGVNMAISINNTNDPLDLSGNIFKDPTDGNIAAGK
                     ELLYIREVQRQTDVYGDVVQEASNLGKNLSSLYPTGTAPGTSLANALKIVARLIDGGL
                     QSRIYWVSTGGFDTHSNQVNTSDTSTGTHATLLQGVSDAIYAFMDDIKLLGKEDRVAG
                     MTFSEFGRRIKSNGSGGTDHGSAQPMFIFGKKVVPGMVGENPTIDPNATSNTNLAMQY
                     DFRSVYASVLQDWFCVPEPDLDTIMLNNYQTLPILDPSGCKTTSTHQMNNNAGENLIS
                     AYPNPFVDKTNIKFESKGGHTMVQVFTAEGYLVRTLIDSTMEAGLYETGCDLSHLPAG
                     MYYIRLQNGTIQQVKSIMKVR"
     gene            complement(2018..3703)
                     /locus_tag="PLE23_04640"
     CDS             complement(2018..3703)
                     /locus_tag="PLE23_04640"
                     /inference="COORDINATES: protein motif:HMM:NF020390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HMX82585.1"
                     /translation="MKNKSNFAPPPGGGISTFTGSFDQAALQHLLRRTLFGASKDDLE
                     YFSGKTLTQVVDELLNVSTVAPDPPIKAYSSKAGGVATDNIDPNVPFGTTWVNTPFNP
                     TGTPNADVSRRVSWKAWWMGLMVNQQRNLREKMTLFWHNHLSTEADIVNTGIAIYRHH
                     ALLRQHALGNFRTLMYDITIDCAMLKYLNGDKNSKTAPDENYGRELQELFTVGKGPDS
                     GYVEDDVKAAARVLTGWQINNNNFNSPPVSIFNPNRHDTGNKSFSSFYNNKVIQGKTG
                     PTAGTDEINELLDMIFATQEVAKFICRKLYTFFVYYNITPEIESNVITPLADLFRNNN
                     YEIKPVLKALFESEHFFDPENRGCMIKSPVDYVVGQIRELGMQLPDASKYEAQYKMWG
                     ELRAAASNMGQDLGDPPNVSGWPAYYQVPQFHEIWVDTSTYPVRKANTESLNKYGMST
                     NAVMFTDESKNLKVKFDFVAWAQKLDAPDDPNALIEEAVKLLYGVDVSQEVKDYLKTS
                     FLLQGQASDYYWTNAFNLYVTDPNTKDPDAMKVPAILRDLILYMQSAAEYHLC"
     gene            3990..5027
                     /locus_tag="PLE23_04645"
     CDS             3990..5027
                     /locus_tag="PLE23_04645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMX82586.1"
                     /translation="MKKIIMLLLPLTFVYCKNESKKDFKAFGGPPKSSKADGYVVSSG
                     TFSEQIQVPGSVLAYETTDIQSETSGKIVSLNIREGSFYPKGTLIAKINDDDLQAKLS
                     KLKIQLQIAQAQVKRQAELLKINAIGQQEFDAVDLNFKSLQSDIEILKTEIRKTEIRT
                     PYAGVLGFKMISPGAYISPQTKITTISQTDKLKLEFALPERYLPVIRNGIIVNLKLDS
                     SRKVYKAKIYAIEPIISQDSRSLNVRAEIIHPDKFIQPGMFASVDVGLSDKNNVIKIP
                     SQAIIAQARDKKVVVYKGGKAEFKTVVTGIRDASNVEIIEGLTVGDTILITGILTAKP
                     DSKIELTSVKN"
     gene            5039..8104
                     /locus_tag="PLE23_04650"
     CDS             5039..8104
                     /locus_tag="PLE23_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMX82587.1"
                     /translation="MNISHFSLRRPVFAIVLNLLIILFGVIGFKSLGIRDYPAIDPPI
                     VSVRTSYPGANADIIESQITEPLEKAINGISGIKNISSMSSQGSSSINVEFELSEDLE
                     AAANDVRDKVSQAVRSLPSDLDAPPVVSKADASSDAILAMTVRSNTKNQLELTEFANN
                     NLLEKLQTIPGVSSIMIWGEKKYAMRIWLNPVKLSAYGLTPGDVQMALQKESLELPSG
                     KLSGNTTELSVRTFGQMQTEEEFNNLIVKNINGAEIRIRDIGVAVLGPENEETVLKES
                     GIPMIALAVSPQPGANYVEISNEFYKRYQKIKEELPDEFEVNIALDQAKFIKQSILEV
                     EETLVFAILLVILIIYLFFRDWLIAIRPLIDIPVSLIGAFFIMYISGFTINVLTLLAI
                     VLATGLVVDDGIVVTENIYKKMEAGMNKYKAAREGSQEIYFAVISTSITLAVVFLPII
                     FLPGFVGRLFREFGVVVAGAVLISAFVSLTLTPVLNVKMTRSDHKHSWFYHKTEPFFR
                     WMEDSYHKLLTGFFKMRWVALAIIAASFGIIFFIGTNLKSELAPLEDRSQFRLMLTAP
                     EGTSFDYMDKYVDKLSGLLIDSIPEKQIVLSVTSPSFTGSGSANTGFVRVVMVDPDKR
                     LRTQEDIVNAVQKELGNFSEGKAFLIQEQTIAVNRRGGLPVQFVIQNNDFEKIKEALP
                     KFLEEANKNPVFQQVDVDLKFNKPELQVEINRTKAAELGVSVEDIAQTLQLAYSNRRL
                     GYFTRLGKQYQVIGQVDRNNRDQPADLKQLYVRNKAGQSISIDNLVTIVETVTTPTIY
                     HYNRYKSATISAGLAPDKTLGDGIKAMREIGDKVLDQSFSTALSGSSRDFEESSGSVG
                     FAFTLALILIFLILAAQFESFIDPIIIMVTVPLAIAGAVLSLWIFDQTLNIFSQIGMI
                     MLIGLVTKNGILIVDFANKQQAAGVDKKTAAIEAATMRLRPILMTSLAMALGALPLAI
                     SIGAASTSRIPLGIVIIGGIIFSLILTLLVVPVLYSFISSKKHELHEDL"
     gene            8091..9392
                     /locus_tag="PLE23_04655"
     CDS             8091..9392
                     /locus_tag="PLE23_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMX82588.1"
                     /translation="MKIFKMICLMAFGLNVFGQTPISLEDAIKEAIKNNLDIAIERNE
                     YVLTGINNSWSNAGAYPTIQADLTPTISSSNLNQKLASGLDINRNGVVAKNFNAEVNA
                     NWNVLNGFKLYTTKKRLEQLEQIGELNLIQQINNLSYEVALTYAAAQQLIQQQKNIEE
                     QISITQERIDINQKKVEVGASGKSDLLQAQIDKNSLISAREALAGQYTNTLISLGLLM
                     GRSPGDYPAVKETMSLPVTEPLDAVIQLALKQNPTILATNKQIAVTALTQKETKAVRL
                     PNVNVRTGYGYNRSQSTGGFSLFNQSYGPTASLGISVPIYSGGRVKSVLEQLDIQMKN
                     QQLNYNLLILEYKTMITQAYNDVKMAEARILLVKDNIKLAKENLEISQGRAKYQSITA
                     LELRQAQYSLMELQTELINNTFLARAAKLKLTMLSGGSNWQ"
     gene            9511..10917
                     /locus_tag="PLE23_04660"
     CDS             9511..10917
                     /locus_tag="PLE23_04660"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX82589.1"
                     /translation="MEIKFCGAAREVTGSSHLITLQDGYRILLDCGLYQGADEEMDDF
                     NDKWLFNPSKIDCLILSHAHIDHCGRIPKLVKDGFRGKIYATPATRDITSIMLMDSAK
                     IQENDAKYHNEKIVKKHKLSAKQSKPLYTIQDAQYAMRFFATYNYEVWYHVHPKVRVK
                     FRDAGHILGSASVTLEISEGDRTIMLGFTGDIGRPDRPILKDPVPMPQCDYLISESTY
                     GDRDHKSAPHDTERLKEIIYDTCINNKGRVIIPAFSLGRTQEILYIMDQLYNEGKLPK
                     VPVYLDSPLAINATEIYRLHPECFDDELHDYMLTDPNPFGFNLLQYVKTVDVSKSLNT
                     DHRQCVIISAAGMANAGRVKHHIFNNIEDPNNTILIVGYCTPFTPGGVLRSGAKEITL
                     FGQVKQVNAKVEIMDSFSAHGDRHEMLDFIKNQKESLKTLYLVHGEEETQKHYKTFLA
                     EAGFKNIEIPRLAQVVKI"
BASE COUNT         3357 a         2260 c         2331 g         3130 t
ORIGIN      
        1 gacaccctta tttggtcata ttcaaaagaa aatgctcctt tttcagtaaa aatatttgaa
       61 aatggcaatc cggattatgg ctattggttt aaactgaacc ctgataaggt gctgtacaag
      121 ccaggtgtcg tatattttat acaaggcccg ggagaacctc ctttcctaga ttttgaggga
      181 gggccaatta cacctacaac acccggtgta caatcttact ttgattacta cttaaataat
      241 aacatattaa caacaggcga tacccttatc attggccagt taaatgcaga atacaagcct
      301 tgagcgtgac atattttgta tcctgactga cttaaaatca taaccaattc tacacggaga
      361 ctcctcagcc aaaggccatt atcttacctt cataatgctt ttcacctgct gtattgttcc
      421 gttctgtagc ctgatgtagt acatgcctgc aggcaagtgg ctcaggtcgc atccggtttc
      481 atacaatcct gcttccatag ttgaatctat caacgtacgc accagatagc cttcagctgt
      541 gaatacctgt accattgtat gcccgccctt actttcaaac tttatattcg ttttgtcaac
      601 gaacggattc ggataagctg aaataagatt ctcacctgca ttattgttca tctggtgggt
      661 actggttgtc ttacaacctg atggatcaag tatcggcaat gtctggtaat tattaagcat
      721 aatggtatcg aggtcaggtt cgggtacaca gaaccagtcc tgaaggacag aggcatatac
      781 agagcggaag tcgtattgca tggccagatt ggtgttggaa gtggcgttag gatcaatggt
      841 cggattttcg cctaccattc ctggaacaac ttttttacca aaaatgaaca ttggttgtgc
      901 tgatccgtgg tctgtaccac ccgaaccgtt tgattttata cgtctgccga actcagagaa
      961 tgtcataccg gccactctgt cttctttacc aagaagttta atatcatcca tgaatgcgta
     1021 tatggcatca gatacacctt gcaaaagtgt agcatgtgta cctgttgaag tgtcactagt
     1081 attgacctga ttggagtgtg tatcaaatcc gccggtactc acccagtaga ttcttgactg
     1141 caacccgccg tcaatgagcc tggctactat cttcagggca ttagccagag aagtgcccgg
     1201 agctgtaccg gttggatata gagaagagag atttttgccc agattgcttg cttcctgtac
     1261 cacatcgcca tatacatctg tctgtctttg tacctcccta atgtagagca attccttacc
     1321 tgcagctata ttgccatctg ttgggtcttt aaaaatattt cccgacaaat caagcggatc
     1381 gttggtatta ttgatagaaa tggccatgtt gacacccata ttctgcaggc caagtcctac
     1441 atttccacca attcttatgc cgagtggatg tggtacggta gcattcggat agccaaccgg
     1501 gaaattagga tattcgtagt ttagatatct gcctgcccac cctgagttaa gaatctggtc
     1561 agaatctgac ccggtcatca ttatgtcggt agctctgaag tgtgaatagt taaaattagg
     1621 gtatccaaca ccctgcacaa tggccagttc gccttcttcc cacatctcag caaagcgggt
     1681 cattgcagga ttgagagccg ttttacctcc tgtgccggat agcaccaacg ctttgctttc
     1741 ttctataagt acattggatc ttaattggct taatgcacta tattggtcaa gcggaatgac
     1801 tgtattaagg ccgtcatttc caccactcag ctgcacaata accaatacgt ggtcggtgtt
     1861 ggtgaggttg gcagaaaggg cagatagcaa agggcttgcg ccataggcgt gaacagtaag
     1921 accaccaatg gctacaggta tagttttgag gaaatttctt cttttcattt tattgatttt
     1981 tgaagcgttt gttaataata attgcaatgc gtcttcatta gcatagatga tactcagctg
     2041 ctgactgcat atacaatatc agatcgcgca atatagccgg cactttcatg gcatcggggt
     2101 ctttggtgtt tgggtctgtg acatataggt taaatgcgtt ggtccagtaa tagtcagatg
     2161 cttgcccctg tagcaggaat gaagttttta aatagtcctt tacctcttgt gaaacatcca
     2221 caccatacaa caactttacc gcttcttcga tcaaggcatt tggatcgtcg ggtgcgtcca
     2281 gcttttgagc ccatgccaca aaatcgaatt tcacctttaa gtttttactc tcatcggtaa
     2341 acatcaccgc atttgttgac atgccatatt tgttcaacga ctcagtattg gcttttctta
     2401 ccggataggt ggaagtgtca acccatattt cgtgaaattg tgggacctga tagtaagcag
     2461 gccagcctga aacattaggc ggatctccaa ggtcctgacc catattgctg gcagctgcac
     2521 ggagttcgcc ccacatttta tactgtgctt cgtattttga agcatctggt aactgcattc
     2581 ctaattctct gatttggcca acaacataat ccaccggact cttaatcata cagccacggt
     2641 tttcggggtc gaagaaatgc tcactttcga acagggcttt gagaaccggt tttatttcat
     2701 aattattatt gcggaacaaa tcggcaagcg gtgtgattac attggattct atctctggtg
     2761 taatattata gtacacaaag aaagtatata acttacggca aatgaatttt gctacttctt
     2821 gtgtagcgaa tatcatatcc aacagttcat ttatttcatc ggtacctgca gtagggccgg
     2881 tttttccttg gataacttta ttgttgtaga atgatgaaaa actcttattg ccggtatcat
     2941 gcctgttggg gttaaaaatg cttacaggcg ggctgttgaa attattgttg ttgatctgcc
     3001 atccggtcaa tactcgggca gccgctttaa catcgtcttc tacatagccc gaatccggtc
     3061 cttttcctac ggtgaataat tcctgaagct cgcgaccgta gttttcgtca ggcgctgttt
     3121 tgctgttttt atcaccattg aggtacttaa gcatggcaca gtcaatggtt atatcataca
     3181 tcagggttct gaaattgccg agtgcgtgct gtcggagcaa ggcatgatgt ctatatatgg
     3241 ctattcctgt attgacaata tcggcttctg tagaaaggtg gttgtgccaa aacaaggtca
     3301 ttttttcgcg caggttgcgt tgctggttta ccattagacc catccaccag gctttccagc
     3361 ttacacgtct cgatacatct gcattcggtg tgcctgtagg gttgaatgga gtgtttaccc
     3421 aagtggttcc gaatggtaca ttggggtcga tgttgtcagt agcaacacca ccggcttttg
     3481 aagaatatgc cttaatcggc ggatcaggtg ctacagtact tacatttaat aattcatcca
     3541 ctacttgtgt cagggttttt ccactgaagt attccaaatc atctttcgaa gcaccaaaaa
     3601 gtgtacgacg gagcaaatgt tgtaatgctg cctgatcaaa actaccagtg aaggtactga
     3661 tacctcctcc cggaggcggg gcaaagtttg atttattttt cataagcatt cagtttataa
     3721 taacggttag acagaatatt ctgtaaaggt ttaatcaggg ttcaagattt aattggcaat
     3781 ttatatattt tatttttcaa aataaattaa aattgaatga ttcaattcat aattcgtctg
     3841 atacaaggat tcctcaaacc cggattttgt tattttagtc ggataaaatg tcgttgtaat
     3901 gattttagaa tcaatttttt agaacaaaat tgctcatagt ttgtagttta gttttgatta
     3961 ttcacaagta agtttttgag tacaggtgta tgaagaaaat aataatgtta ctgttgccac
     4021 tcacttttgt ttattgtaaa aacgagagca aaaaagattt taaagcattc ggcgggccac
     4081 ccaaatcgtc aaaggctgat ggttatgttg tcagttcggg cacatttagt gaacagatcc
     4141 aggtgccggg ttcggtgctt gcctatgaga ctactgacat tcagtcagaa acatcgggca
     4201 agatcgtttc gctcaacatc cgtgagggat cattttaccc aaagggtaca cttatagcaa
     4261 aaatcaatga tgatgatctg caggccaaac tcagtaagtt gaagatacag ttgcagatag
     4321 cacaggcaca ggtaaagcgc caggccgagt tgctaaagat taatgctatc gggcagcagg
     4381 aatttgatgc tgttgatctt aattttaaaa gtttacagtc agatattgaa atactgaaaa
     4441 cagaaatcag gaaaacagaa attcgtacac catacgcagg ggtacttggc ttcaaaatga
     4501 taagtccggg ggcatacatc tcaccacaaa ccaagataac caccatatca caaactgata
     4561 aactcaagct tgaatttgca ttgccggagc gttatcttcc tgtcatccgt aacggtataa
     4621 tcgtaaacct gaaactcgat agttcacgca aggtttataa agcaaaaata tatgcgatag
     4681 aacccattat atcacaggat tcaagaagcc ttaatgtacg tgcagaaata atccatcctg
     4741 ataaattcat tcagccggga atgtttgcga gtgttgatgt tgggctcagt gataaaaata
     4801 atgtgataaa gataccaagc caggccatca tagcacaagc ccgtgataaa aaggttgttg
     4861 tttacaaagg aggaaaagcg gagttcaaaa ctgttgtgac gggcatacgc gatgccagta
     4921 atgttgaaat aatcgaaggc cttaccgtag gtgacacaat attgattaca ggtatcctga
     4981 ctgccaaacc tgattcgaaa atagaactga cttccgttaa aaattaatac cacagaagat
     5041 gaatatttct cattttagtc tcaggaggcc ggtttttgca atagtattaa atctgttgat
     5101 catacttttt ggtgtcatcg ggttcaaatc gcttggtata agggactatc cggccattga
     5161 tccgccaata gtatcagtaa gaacatcata cccgggagct aatgcagata tcattgagtc
     5221 gcaaataaca gagcctctcg aaaaagccat caatggtatc agtgggataa aaaatatttc
     5281 atccatgagt agccagggct cgagctccat caacgtcgaa tttgagctaa gtgaagatct
     5341 ggaagcagct gccaatgatg tacgtgataa agtatcacag gctgtgcgtt cattaccttc
     5401 agatcttgat gctccacctg tagtgagcaa agcagatgcg agttctgatg ccattcttgc
     5461 catgacggta cgcagcaata caaaaaatca gctcgaactg acagaatttg ccaataacaa
     5521 cctactcgaa aagctacaga caatacccgg cgtgagtagt attatgattt ggggtgagaa
     5581 aaaatatgcc atgcgtatat ggctcaatcc ggtaaaatta agtgcttatg gcctgacccc
     5641 gggtgatgtc cagatggctt tgcaaaaaga aagccttgag ttgccttccg gaaaattatc
     5701 cggcaatact acagaattat ccgtacgtac ctttggccag atgcagaccg aagaagaatt
     5761 caataacctg atagtaaaaa atataaacgg tgccgaaatc aggatccgtg atataggtgt
     5821 tgccgtactc ggtccggaaa atgaagaaac tgtattaaaa gaaagtggaa taccgatgat
     5881 tgcccttgct gtgtcacctc agccgggagc aaattatgtt gaaatatcaa atgaattcta
     5941 caaaaggtat cagaaaatta aggaagaatt acctgatgaa ttcgaagtaa atattgccct
     6001 tgaccaggca aaattcatca aacagtctat cctggaagta gaggagacac tggtttttgc
     6061 cattcttttg gtaattctta tcatttattt gttcttcaga gactggctaa ttgccatacg
     6121 tccgcttatt gacattccgg tgtcattgat tggggctttc tttattatgt acatttccgg
     6181 atttacaatc aatgttttga cattgttagc catagtactg gccaccgggc ttgttgtaga
     6241 tgatggtatc gtcgtcacgg agaatatcta taaaaagatg gaggcaggaa tgaataaata
     6301 caaagcagcc cgtgaaggct cgcaagagat atattttgcc gttatttcta cttccataac
     6361 ccttgctgta gtattccttc ctattatttt tctacctggt tttgtgggta gattgtttag
     6421 ggaatttggt gtggtagttg ccggggcagt actcatttct gcttttgtat cacttacact
     6481 gacaccggtg ctgaatgtaa aaatgacaag atctgaccat aagcattcat ggttttacca
     6541 taaaacagaa ccattcttca ggtggatgga agactcttat cacaagctgt tgaccggatt
     6601 ctttaagatg cgttgggtag cgctggctat cattgctgca tcttttggta taatattctt
     6661 catcggtacc aacctaaagt cggaattggc acctcttgaa gacagaagcc agttcagact
     6721 gatgctgact gcaccagaag gtacttcgtt tgactatatg gataaatatg ttgataaatt
     6781 atctggattg cttatagatt cgatacccga aaagcagata gtactttctg ttacatcacc
     6841 atcctttacg ggttcgggct ctgccaatac gggtttcgta cgagtagtaa tggtggaccc
     6901 ggacaagagg ctccgtacac aggaagatat agtcaatgcg gtacagaaag aactcggcaa
     6961 tttttcagaa ggcaaagcat tcctcattca ggaacagacc atagccgtta acagaagagg
     7021 aggattgccg gtacagtttg ttatacagaa taacgatttt gagaaaatta aggaggcgct
     7081 tccgaaattt cttgaagagg ccaataaaaa tccggttttc caacaagtgg atgttgacct
     7141 caagtttaac aaaccggagc tacaggtgga aataaacaga accaaagcag cagagctggg
     7201 tgtgagtgtt gaagatatag cacaaaccct ccagttggca tatagcaacc gtaggttagg
     7261 atattttaca agattaggta agcaatatca ggtaataggc caggtggacc gaaataaccg
     7321 tgaccagcct gctgacctta agcagttgta cgttaggaat aaagcaggac aatccatttc
     7381 aatcgataac ctcgtaacaa ttgttgagac tgtgactacc ccaactattt accattacaa
     7441 tcgctacaag tctgcaacca tttcggcagg tcttgctccg gacaaaacac ttggtgatgg
     7501 tatcaaagcc atgcgtgaaa taggtgataa agttttggat cagagttttt ctaccgcttt
     7561 atctggatca tcaagagatt ttgaggaaag ttcaggaagt gttggctttg cctttacatt
     7621 ggcacttata ttgattttcc tcattctggc tgctcagttc gaaagtttca ttgatccaat
     7681 tatcattatg gtgaccgtac cattggcaat tgcaggagcc gtcttgtcat tgtggatatt
     7741 tgatcagacg ctcaatattt tttctcagat aggtatgatt atgctcatag ggcttgtgac
     7801 aaaaaatggt atcttgattg tggactttgc caataaacaa caggctgccg gagtagataa
     7861 aaaaactgct gccatcgaag cagctactat gcgactaaga ccgatactca tgaccagcct
     7921 tgctatggca cttggtgcat tgccactggc catttctata ggtgctgctt ctacgagcag
     7981 aataccactg ggtattgtta tcatcggtgg tataatattc tcccttattt taaccttatt
     8041 ggtagtacct gttttgtatt ctttcatttc atcaaaaaaa cacgaactgc atgaagatct
     8101 ttaagatgat ttgtttgatg gcttttggct tgaatgtatt tggccagacg cccattagcc
     8161 ttgaagatgc tataaaggag gcaattaaga ataatcttga catagcaatc gaaagaaatg
     8221 aatatgtgct taccggtatc aacaacagct ggagtaatgc gggggcgtac ccaacaatac
     8281 aggccgacct tacaccaacc atctcgagta gcaatctgaa tcaaaaactg gcaagcggac
     8341 tcgatattaa tagaaatggt gtggtagcta aaaattttaa tgctgaagta aatgctaact
     8401 ggaatgtact gaatggtttt aaattgtaca ccaccaaaaa aagacttgaa cagctcgaac
     8461 agatcggtga gttaaatctt attcaacaaa tcaacaatct gtcgtatgaa gtggcattaa
     8521 catacgctgc ggcacaacaa ctcattcaac aacaaaaaaa tatcgaagaa caaatatcca
     8581 tcactcagga aagaatagat atcaatcaga aaaaagtaga agttggcgcc tcaggcaaat
     8641 cagacttact acaggcacag atagataaga attcgcttat cagtgcacga gaagcccttg
     8701 caggacaata cacaaacacc ctgatttctc ttggactatt gatgggtagg agcccgggcg
     8761 actatcccgc agtaaaagaa accatgtcat tgcccgtcac agaaccactt gatgctgtaa
     8821 tacaactagc cttaaaacaa aaccccacga tactggctac caacaaacaa attgccgtaa
     8881 cggcactcac acaaaaggaa accaaagcag tcagactgcc aaatgtaaat gtaagaacag
     8941 gctacggcta caacagaagc cagagcaccg gtggattcag tttatttaac caatcgtatg
     9001 gtccgactgc atcacttggt atctcagtac cgatttatag tggaggccga gtgaaaagtg
     9061 ttctcgaaca gctagatatt caaatgaaaa accagcaatt gaactacaat ctgctgatac
     9121 tcgaatacaa aaccatgata acacaggcat ataatgatgt aaaaatggca gaggcaagaa
     9181 tattactggt aaaagacaac atcaaactgg caaaagaaaa cttagaaata tcacaaggta
     9241 gggcaaaata ccagagtatt acagcacttg agctaaggca ggcacaatac tcattgatgg
     9301 aattgcagac agaattaatc aacaacacat tcctggcaag agccgcaaag ttaaagttga
     9361 caatgctcag cggtggtagt aactggcagt aaaagttgaa tgatatttta aggaaagaaa
     9421 aacctttctt acacccattg ttcttcaaca attcttccaa tattgaaatt tttatttacc
     9481 tttagcacct gaatattcaa ccagataaaa atggagataa agttttgtgg tgcagccaga
     9541 gaagtaaccg gaagttcgca ccttattaca ttgcaggatg ggtaccggat tttgctcgat
     9601 tgtggtcttt atcagggtgc cgatgaagaa atggacgatt ttaacgataa atggcttttc
     9661 aacccgtcca aaatagattg cctcatatta tcacacgctc atattgacca ttgcggacgc
     9721 atacccaaat tggtgaaaga tggttttagg ggtaaaatat atgctacgcc agccaccaga
     9781 gatattactt ccatcatgtt aatggatagc gccaaaatac aggagaatga cgcaaaatac
     9841 cacaacgaaa aaatcgtaaa aaaacacaaa ttatcagcta agcaatctaa gcccctttat
     9901 actattcagg atgctcaata tgctatgcgc ttctttgcca cctacaacta cgaagtttgg
     9961 tatcacgtac atcctaaggt cagggttaag ttcagagatg caggccatat cttgggttcc
    10021 gcttcagtaa cacttgaaat atctgaagga gatcgtacca ttatgcttgg ttttacgggt
    10081 gatatcggca gacccgaccg cccaatactc aaagatccgg tcccaatgcc acagtgcgat
    10141 tacctcatca gcgaatcaac atacggagac cgtgatcata aatctgcgcc acatgatacc
    10201 gaacggttga aggagataat ctatgatacc tgtataaata ataaaggacg tgtgattatt
    10261 ccggctttct cattgggacg tacacaggag atactctata tcatggatca attgtacaat
    10321 gaaggcaagc taccaaaagt accggtgtac ctcgacagcc ctcttgctat aaatgccacc
    10381 gaaatatacc gtctgcatcc ggaatgtttt gatgatgaac tgcacgacta catgcttact
    10441 gatcccaacc cttttggctt caatctgcta caatatgtca aaactgtaga tgtgagcaaa
    10501 agcctcaata ccgaccatag gcagtgtgtc atcatcagtg ctgcaggtat ggccaatgcc
    10561 ggtagagtaa aacaccacat ttttaataat attgaagatc cgaacaatac aatccttatc
    10621 gttggttatt gtacaccatt tactccaggt ggggtattgc gctccggtgc caaagaaatt
    10681 accttgttcg gtcaagtaaa acaggtaaat gcaaaggtgg aaattatgga ttcgttttct
    10741 gcacatggcg accgacacga aatgcttgat tttattaaaa accaaaaaga atcactcaaa
    10801 actctatacc tcgtacatgg cgaggaagaa acacaaaaac attacaagac attcctggca
    10861 gaagcaggct ttaaaaatat tgaaatacca cgattggcac aagtagtaaa aatatgaaaa
    10921 atttacgata tcttcttatt ccggcaatag ccgccatact agtagtcttg agcgcctgca
    10981 aaaaagattt tgatgacctg gaggtaaaat ctgatgccga atatgcattt ccactttttt
    11041 ttggcgacct aaaactccaa aaactcatag agaacagc
//
LOCUS       DAOQQY010000060        11067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2121_length_11067_cov_6.063204, whole genome shotgun sequence.
ACCESSION   DAOQQY010000060 DAOQQY010000000
VERSION     DAOQQY010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11067
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2121_length_11067_cov_6.063204"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2329)
                     /gene="leuS"
                     /locus_tag="PLE23_04665"
     CDS             complement(<1..2329)
                     /gene="leuS"
                     /locus_tag="PLE23_04665"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMX82590.1"
                     /translation="MEYTPGFIEAKWKQKWKEADIYKVEIDRSKPKYYILDMFPYPSG
                     AGLHVGHPLGYIATDIFSRFKRHQGYNVLHPMGFDAFGLPAEQYAIQTGVMPQISTEQ
                     NIKRYREQLDNLSFSYDWSRSVTTSDPAYYRWTQWIFIRLYEHYFNTKENKAKPVSEL
                     IEVFEKSGSEQAAAFSTIEDHFTAQQWKAFSPKQKDDVLMHYRLAYKGQAFVNWCEEL
                     GTVLANDEVKDGLSERGGYPVVQRPMEQWFLRITAYADRLLNDLKTLEWTDALKSMQE
                     NWIGKSEGAQVFFKIKDSEESIEIFTTRPDTIFGASFIVLAPEHDLVEKLVTPEQQHT
                     VKNYLDYAKTRSERERISETKQVTGAFTGAYAVHPFTGESIPVWISDYVLKDYGAGAI
                     MAVPAGDLRDHAFAEKFSLPIIEIIDRSAHPKATIEDKLGTMINSGFLNGLEVKDAIK
                     MAIGEIATKGIGKAKVNFRLRDANFSRQRYWGEPFPVCYDEDGICHPLSDTDLPLMLP
                     ELEDFRPGAGSQSPLARVPAWQSTREGYRRETDTMPGFAGSSWYFLRYMDACNPDELV
                     SHEAIDYWQNVDLYVGGTEHAVGHLMYARFWHKFLFDLGIVNTQEPFRKLINQGMIQG
                     RSNFVFRAKENFFEEYLKINVLDKYFADSGVYRLTEDEYDEEFCADWAFKSNDLVIEV
                     VSWKLEDKIERIKGAVLKAGKRLLIITNEELTMSINHPLVIAEKIRTAIDGSENFIFD
                     KSEEIDSQLYVSYNLTGNYSADCFSKIHVDVNIVHN"
     gene            2648..3661
                     /locus_tag="PLE23_04670"
     CDS             2648..3661
                     /locus_tag="PLE23_04670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX82591.1"
                     /translation="MTQNTNFSSTINISLIVPVYNRPDEVRELLESLSKQIDMDFELM
                     IVEDGSTLPCSDEVKAYEGKMKIKYFAKENTGPGITRNYGYERCTGNYGIFLDSDCII
                     PPHYIATVKKSLTNQYVDAFGGPDKADESFSILQKAIDYTMTSFITTGGIRSGSKKLD
                     KYYPRSFNMGISREVFESTGGFPAVKFANTKAAGEDIELSIMIYKAGFKIGYIAEAYV
                     YHKRRTSLKMFLRQVFNFGYARVSLSRRNKGTLKMVHLLPLLFTLGTGALLLMSVLFS
                     WKFLWPLMVFSLIVFLDALLKKKSIRIAGTALLTSYIQLIGYGCGFISGWWELCVLKK
                     ENI"
     gene            3848..4474
                     /locus_tag="PLE23_04675"
     CDS             3848..4474
                     /locus_tag="PLE23_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82592.1"
                     /translation="MKGATYILLVILTCFGSCGPAIYQSSNFDKKQRKIKIIAILPFS
                     VNIDMHHLPKGITPENIRNAEEKTGYDLQSSSYTWFLKRQDKYTTVFQDIDKTNAILR
                     KEKISYSDIALQDKSKICKLLGVDAVISGRSSMSKPMTEIAAVAVGLISGAWGPTNSI
                     SNVLTIHDMKAKLLWKYDHNASGSVLTSPDSLIKSLMKNASRKFPFKK"
     gene            4533..5288
                     /locus_tag="PLE23_04680"
     CDS             4533..5288
                     /locus_tag="PLE23_04680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX82593.1"
                     /translation="MQAISAVIITYNEERNIARCLESLLQVVDEIVVIDSYSTDRTEE
                     ICRQYGVRFISHEWEGYSGSKNFGNSQATHDLILSLDADEALSPALQKSILTIKNDES
                     TFFYRIGRITNYCGHWIHHSGWYPDVKLRIFDRRISSWEGHIHEKLNNFNPKDAPLLK
                     GDCFHYSYYTLEDHKAQARKFSMLAAEDLFWRGKRAGWFKILLSPVHKFIKIYLLQLG
                     ILDGIAGLRIAYISAHAVYLKYHTLKKLHASSK"
     gene            5272..6273
                     /locus_tag="PLE23_04685"
     CDS             5272..6273
                     /locus_tag="PLE23_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMX82594.1"
                     /translation="MPVASKRPIGTRIIISRTDSIGDVVLTLPLAGIIKEYFPEWHII
                     FLGRNYTREIVSLSEHVDEFVNWDDHEAVKQLKADIIVHVFPVKEIAQWAKKQGISTR
                     IGTTGRLYHWLYCNKLIRLSRKNSDLHEAQLNIKLLKPLGIETDYPLEKIPDYYGFTR
                     IKPLAPKWLEMLKGDRKNIILHPKSKGSAREWGLENFSRLIGLLPEIEYRIFISGTKE
                     EGLQSAPLFKEHAHVIDLTGQMNLGEFASFIAEADALVAASTGPLHVAAALGKCAIGL
                     YAPMRPIHPGRWAPLGRQASYLVMNRECNDCKKTLDCRCIRDITPGQVSEILSQLRK"
     gene            complement(6608..7174)
                     /locus_tag="PLE23_04690"
     CDS             complement(6608..7174)
                     /locus_tag="PLE23_04690"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMX82595.1"
                     /translation="MLETERLLKDKETAAGRTMEEAVMLLNHKEAIDFLVAHPDYQSP
                     LSVSKIEDIHSILIKELAVDRNLRKRRVGISGTNYRSLDNEFQIREAIDSSCKLVNDK
                     ENVFEKALIALVLISYIQPFMDGNKRTTRIVSNAILMHYKYCPLSFRTVDSVEYKKAM
                     LLFYEQNNISAIKDIFIKQFEFAVKTYF"
     gene            complement(8307..8954)
                     /locus_tag="PLE23_04695"
     CDS             complement(8307..8954)
                     /locus_tag="PLE23_04695"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMX82596.1"
                     /translation="MKKILLLFVLFILSTHVANAQIKTYFKADAVGSVVNSKVEKLDG
                     TYKRIDHFYTPLIRPTIGFGLEYQFADNGVKLVTGLEWTQRGYREPDAKYDTIPGVVR
                     GQISDMLSFPVNIGYRINDKFSVLAGGRVDKNIWKNKNYLVIYEDYTGGNVYNSITAG
                     ANLGIEYHFNKNITVGAEYCHNFTSFTKDEPLVFKTKVESYYRSLSLYARFSFMK"
     gene            complement(8954..9589)
                     /locus_tag="PLE23_04700"
     CDS             complement(8954..9589)
                     /locus_tag="PLE23_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82597.1"
                     /translation="MMFHCVVAQSRLDYTVYAGPNYSCISTLFFYNQPVYTGSEGPLF
                     GLKYQIGGKLGYGFNDIWKVRLGVRFIERSSPKGDGYYPDVIFNSWHKCLGATSEIVF
                     TPKNSRLSLVAGINYDLAITSNTSLPSLGEPHTAFKGLGSRTKYKNYFFLWSFGGEYR
                     LTKSWAIGLHFERAFTPYSVKDTNDPFFFGVKNTVISRCPQGFEFNLNYTF"
     gene            complement(9689..10435)
                     /locus_tag="PLE23_04705"
     CDS             complement(9689..10435)
                     /locus_tag="PLE23_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82598.1"
                     /translation="MATDSIEELYVISTFNNKVQFQAEDSSFTNKFLTNLEFANMNGE
                     FKIGTSLCKEYNGYLLQVLDGDDSKMLNAMTTLETDTLYGVFATEIIVRGGNCHDGCP
                     LLFTDSKTVDKSRLNIQYFIEPRFTYFSDPFSSGYYWKYPAYDFVFQIKNEKKGFLGF
                     WYGAKLYNNYKIDFKITHACNITPNPYIASVYGVTDKARATYERRYGFANQPVLALVD
                     FEPEFCLKYVRFSGSRTKSPYIALETRCNR"
     gene            complement(10460..10798)
                     /locus_tag="PLE23_04710"
     CDS             complement(10460..10798)
                     /locus_tag="PLE23_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82599.1"
                     /translation="MKKRRTEQNRTGFHVKDYIFITFILSIILLLSSCKKDDIQTNKS
                     NVVDNTIPNPFVGNRTTNEIGNFTINNGRICFNTFADYIQTLNFINLADSLELEQYRS
                     SLSIETPNIA"
BASE COUNT         3413 a         2282 c         2088 g         3284 t
ORIGIN      
        1 cattgtgtac aatatttaca tcaacatgta tttttgaaaa acaatcggcg gaataattgc
       61 cagtcagatt gtacgaaacg tacagctgcg aatcaatttc ttcactttta tcgaatatga
      121 aattctcact accgtctata gcagttcgaa ttttttcagc tattaccaaa ggatggttga
      181 tgctcatggt gagctcttca tttgttatga ttaacagacg tttgccggct ttaagtacag
      241 caccttttat tctttcaatc ttgtcttcga gcttccacga cactacttct atgaccaggt
      301 catttgattt gaatgcccaa tcagcacaga attcttcatc gtattcatct tcggtcaggc
      361 gatacacacc agaatcagca aaatatttgt ccagtacatt tatcttaagg tattcttcga
      421 agaaattttc tttggctctg aacacaaagt tcgatcgccc ttggatcatc ccttgattga
      481 tcaattttct gaatggttct tgtgtattga caatgcccag atcgaacaga aacttgtgcc
      541 agaatcgtgc atacatcaag tgaccgacgg catgttctgt tcctccgacg tacaaatcta
      601 cattctgcca ataatcaatt gcttcgtggc tgacaagttc atccggatta caggcatcca
      661 tataacgcag aaaataccag cttgagcctg caaaccccgg catagtgtct gtctcacgac
      721 ggtagccttc tcttgtgctc tgccaggctg gtacacgagc aagtggtgac tgtgaaccgg
      781 cacccgggcg aaaatcttcg agctccggta acataagtgg caggtcggta tctgataatg
      841 ggtggcaaat accatcctca tcatagcaaa ccgggaatgg ctctccccaa tatctttgtc
      901 ggctgaagtt ggcatcacgc agcctgaagt tgactttagc cttgcctatg cctttggtgg
      961 cgatctcacc gattgccatt ttgatggcat cctttacttc gagtccgttc aagaatccgg
     1021 agttgatcat tgttccgagt ttgtcttcga ttgtagcctt cggatgcgcc gaacggtcga
     1081 tgatttcaat aattggcagg gagaattttt cggcaaatgc atggtctcta aggtcgcctg
     1141 ccggcacagc cattattgca ccagcaccat aatctttcaa tacataatca gatatccata
     1201 ccggaatact ctctccggtg aatggatgaa cggcataagc gcctgtaaat gcaccggtca
     1261 cttgtttggt ttcggaaatc cgctctcgtt ctgaccttgt tttggcataa tcgaggtaat
     1321 tcttaactgt atgttgctgt tcgggcgtga cgagcttctc taccaggtcg tgttcgggag
     1381 cgagtacgat gaagctggcg ccaaatattg tatccggtct ggttgtaaat atttctattg
     1441 actcttcgct gtccttgatt ttaaaaaata cttgtgcacc ttccgatttg ccgatccagt
     1501 tctcctgcat acttttgagt gcatctgtcc attccagcgt tttcagatcg ttgagcaatc
     1561 tgtcggcata agcggttatg cgcaaaaacc attgttccat gggtctttga acaacaggat
     1621 agccaccccg ctccgacaga ccatccttca cttcatcgtt ggcgagtacg gtaccgagtt
     1681 cttcgcacca attaacgaag gcctgccctt tgtaggcaag ccgataatgc atcagcacat
     1741 cgtctttttg ttttggtgaa aatgctttcc attgttgggc tgtaaaatga tcttcgatag
     1801 tagagaatgc agctgcctgt tcggagcctg atttttcgaa tacttcaata agctcactta
     1861 caggttttgc tttattttct tttgtattga aataatgttc gtatagccga atgaatatcc
     1921 attgtgtcca gcgataataa gccggatcgg atgtagtgac cgacctcgac cagtcgtaac
     1981 taaagctgag gttatccagc tgttcgcggt accgtttaat gttttgttct gtagaaatct
     2041 gtggcatgac gcctgtctgt atggcgtatt gttcggcagg taggccaaat gcgtcaaaac
     2101 ccatcgggtg aagtacatta taaccttgat gacgcttaaa tcttgaaaaa atatcagtag
     2161 caatataacc cagcggatga cccacgtgaa gaccggcacc agacggataa ggaaacatat
     2221 ccagaatgta gtattttggt ttggatctgt ctatttccac cttatagata tcagcctctt
     2281 tccacttttg cttccatttt gcttcaataa acccgggtgt atattccatg tacattgtcg
     2341 tttttcaaat ttgctgcaaa agtacgaaat ttacctgatt tagttaaaga atgaaggcgc
     2401 atttgagcca aaaacacagt aaaagtgaat aatatgccaa tacagatgat ttttatttta
     2461 ataaaatgga aggaagtata ggcacttgat ggtaaaatat acaaaaatat actcagatac
     2521 cgcgctgatt tggctttaca ttattcactc aaaatgacct aaccaaagca tatttagcca
     2581 aggcaatctg acagtcgtaa aattggctta tctttgccac cggattacat ttcctgatgg
     2641 aaaaaagatg acacaaaata caaatttctc ctcaacaatc aatatttcac ttatcgtgcc
     2701 ggtgtataac aggcccgatg aagtacgtga attattggaa agtctttcga agcaaatcga
     2761 tatggatttt gagctgatga tagtagaaga cggctctacc ctaccctgct cagatgaagt
     2821 aaaggcttat gaaggcaaga tgaagataaa atatttcgcg aaagaaaata ccggcccggg
     2881 cattaccaga aattacggtt acgaacgatg taccggcaat tatggtattt ttttggactc
     2941 ggactgtatc attcctccgc attatattgc tactgttaaa aaatcactca caaaccaata
     3001 tgtggatgca ttcggaggcc ccgacaaggc tgatgaatcg ttcagcatat tgcaaaaagc
     3061 gatagattat accatgacct ccttcattac tacaggtgga ataaggagtg gcagcaaaaa
     3121 attagacaaa tactacccaa ggagcttcaa tatgggtata tcaagagaag tttttgaatc
     3181 aaccggtgga tttcctgccg taaaatttgc caacacgaag gccgccggag aagatattga
     3241 gctcagcatc atgatatata aagccggatt caagatcggt tatatagctg aagcttacgt
     3301 ttaccataaa aggcgtacaa gtctgaagat gttcttaagg caagtgttca attttggata
     3361 tgcgcgtgtt agtttatcga gaaggaacaa aggaacttta aaaatggtgc atcttttgcc
     3421 attgcttttc acactgggta ccggagcgtt gttgctgatg tcagttctat tttcatggaa
     3481 atttctttgg ccgttgatgg tgttttcact gattgttttt ttggatgcat tattgaagaa
     3541 aaagagcatc cgtatagcag gcacagcatt gctcaccagc tacatccaac tcatcggtta
     3601 tggttgtggc tttatcagtg gatggtggga actatgtgtg ctgaaaaaag aaaacattta
     3661 aaaacaaatc gcaagaaagc ccggacattt tgttgtaact gcccatttgt aggtgttgat
     3721 aaaaaaagtg gaatgataat ttttatcata aagaaaacaa ctcaaaccca gataatgcat
     3781 tagacatttg ggttaaaaga taagtgcggt gtaatattac ttgtacaata aagaaataga
     3841 cagaagaatg aaaggggcga cttacatatt acttgttatt ttgacttgtt tcgggtcatg
     3901 tggacctgcc atttaccaat catcaaattt tgataaaaaa caaagaaaaa taaaaatcat
     3961 agccatattg ccgttcagtg tgaacatcga tatgcatcat ttgcccaaag gcatcacccc
     4021 tgaaaacatc aggaatgcag aagaaaagac cggttacgac ttacagagca gcagttatac
     4081 ctggtttttg aaaaggcagg ataagtacac aacagtattc caggacatcg acaagacaaa
     4141 tgccatactc aggaaggaga aaatctcata cagtgatatt gccttacaag ataaaagtaa
     4201 aatctgcaag cttcttggtg tagatgccgt catatcgggc agatcgtcca tgtcaaaacc
     4261 catgacagaa atagcagccg ttgctgttgg cttgatatcc ggtgcgtggg gccctaccaa
     4321 tagcatcagc aatgtgctta ccatacatga tatgaaagct aagctgctat ggaagtacga
     4381 ccacaatgca agtggctcgg tattaaccag ccctgacagc ctgataaaat cgttgatgaa
     4441 aaatgcatct cgaaaatttc catttaaaaa ataaaccata aggccacagc gtagcgaata
     4501 aagaaaccgc atacatagat tatctttgca ctatgcaagc aatatcggct gtcattatca
     4561 catacaacga agaacgcaac atcgctagat gtctagaatc cttgctgcag gtagtggacg
     4621 aaatagtagt gattgattca tattctacag atagaacgga agaaatttgc aggcaatatg
     4681 gagtacggtt tatctctcat gagtgggaag gatattccgg atcaaaaaac ttcggcaaca
     4741 gtcaggccac gcatgacctt attttgtcat tagatgcgga tgaggcctta tctcctgcgt
     4801 tacaaaagag catcctgact atcaaaaacg atgaaagcac attcttctac cgtattggcc
     4861 gtataaccaa ctattgtggc cattggatac accacagtgg ttggtatcct gacgtcaagt
     4921 tgcgtatatt cgacaggcgt atttcttcct gggaaggcca tattcacgaa aagttaaaca
     4981 atttcaatcc aaaagacgcc cctttactta aaggtgattg ctttcattac agctattata
     5041 ccctggaaga tcacaaggca caggcaagga aattttcgat gctagcagct gaagacttgt
     5101 tttggcgcgg aaaacgagca ggctggttca aaatattgct gtcgcctgta cataaattca
     5161 taaaaatcta tctgcttcaa ttgggcattc tggatgggat tgccgggctg cggatagctt
     5221 atatttcggc acatgccgtt tatttaaaat accatacact taaaaaactt catgccagta
     5281 gcaagtaaaa ggccgattgg caccagaatc attatcagtc gtacggacag cattggcgac
     5341 gtagtactta cattgccgct tgccggtatc atcaaagagt atttcccgga gtggcacatc
     5401 atttttctgg ggagaaacta tacgagagag atagtctcac tatcagaaca tgtggacgaa
     5461 ttcgtgaact gggacgacca tgaagccgtc aaacaattga aagcagacat cattgtacat
     5521 gtcttcccgg tgaaggaaat agcccaatgg gcaaaaaaac aaggcatttc cactcgtatt
     5581 ggcaccaccg gccggctgta tcactggctt tattgcaaca agctgataag gctctcgaga
     5641 aaaaactcag atctgcacga agcacaactc aacatcaaac tactaaagcc attgggcata
     5701 gaaacggatt atccgcttga aaagataccg gattattacg gctttaccag aataaagcct
     5761 ctcgccccaa aatggctgga aatgctgaaa ggagacagga aaaacatcat ccttcacccg
     5821 aaatcgaaag gaagtgcacg agaatggggg ctcgaaaatt tttcccgtct catcggtttg
     5881 cttccggaga ttgaataccg gattttcatt agcggcacca aggaagaagg acttcagtca
     5941 gcgccattat tcaaggaaca cgcacacgtt atcgacctta caggacaaat gaatcttgga
     6001 gagtttgctt ccttcatagc agaagcagat gccctggtag ctgccagcac aggaccattg
     6061 cacgtagctg ctgcgttggg caaatgtgcc atcggcctct atgcccccat gcgacccata
     6121 caccccgggc gttgggcgcc actcggcaga caggcgagct atctggtgat gaaccgagaa
     6181 tgtaatgatt gcaaaaaaac gctggattgc aggtgcatac gtgacattac gcccgggcag
     6241 gtgagtgaaa tactctccca attgagaaag taggctggtg tgctagctta agctaaaaaa
     6301 acaagccctt tggatggatc aaagggctgt gcggaagttg tcactaatct ctgttcatca
     6361 ggtttcaacc ttacgcggca tttcctgatt tctaacttcc ggatatttac tcaacttaaa
     6421 actctaagcc aaaaaaaaat gacaagtatg taagtctgta tattgtgacg cttacaatat
     6481 cggatttgtt gtgtacacat ccaattttaa taaaaatcta atgaaagcta tgattttgtc
     6541 aagatgatac ggttgactga atgacaaatt cataacgaac aggcaattaa caggagttct
     6601 ccgggaatca gaaataggtc ttaacggcaa attcaaactg cttgataaaa atatccttta
     6661 tggcagaaat attgttctgt tcataaaaca gcagcattgc ctttttatat tcaaccgaat
     6721 caaccgtcct gaaagaaagc ggacaatact tgtaatgcat taatatggca ttgctgacta
     6781 tccttgtcgt tctcttgttc ccgtccatga atggctggat gtaggatata agcaccaaag
     6841 caattagtgc cttctcaaat acattttctt tgtcattcac taatttacag cttgaatcaa
     6901 tggcctcgcg tatctggaat tcgttgtcga gcgaccggta attcgtaccg gaaatgccca
     6961 ccctgcgctt gcgcaaattt ctgtcaactg ccagctcttt tatcaggatg ctatgaatat
     7021 cttcaatttt agagacagat aaagggcttt ggtaatccgg atgtgctact aggaaatcaa
     7081 ttgcttcctt gtgattcaac agcataacgg cttcttccat cgttcttcct gcggcggtct
     7141 ctttatcttt gagcaggcgt tctgtctcga gcaaggaact ttctttccct tattgttctt
     7201 cttcaattat cattcttcca acctcaaaat aagtcaaaac catagtatga tttatggatt
     7261 gcacaacttg ttttcgggct tccttgagca agagggaaat cctttcagaa agcctctttg
     7321 gtgtaatatc tgtcatatct gtttcatcca ggttatcata aaaatatatg aaagttggca
     7381 attaacttat caaattaaag tcgttaagca attattgcat aatctatcag aaataggagc
     7441 cgatactaac tcctatttct tatcatcaca atccacaccc atccgcctta cccacatctt
     7501 aaagatttta tccacaagac tttaatgaca aggcagccag aaagccggct gccttcccgg
     7561 ttttacttta acactatgaa aacatagcgt aattatcatc atttactgaa tatggcgcta
     7621 cagctagcta ctgtctcatt cgatggaaga ttatagtgtc tcgcctccac gaggaggttt
     7681 ttaggatcgg acttatctac tgtaaattca atatacgacg acgttttatc acctattaca
     7741 caatgtcttg gtattataaa tctaaacttt gtcggactca ggtttttgat ctatgtgtgt
     7801 ccagtaccat acccgcttga gccgggtaaa gaaacatacg atatactaac ttcattccca
     7861 atactatcta tatttaaaac atacttatcg gtgcaaccac aatcaaatga gccggcataa
     7921 caggattcgc atttagaaat acaactgttt accaataaaa tcttagttga gttgcaggcg
     7981 cattcgccat ccttgcaata ttgttgaaac ccacaaggcg tagtgcactc cttatccatc
     8041 ttcatctttt tgcagccggc acttgccatg acaatacata caaacaatac aactatactt
     8101 cttataatta aaatttttga aaatgattat aaaataaatt tccatttgtg tctttactta
     8161 gtagaatata actacctggt ggtaaatcac caatatgtag ttcattcaat tcttgctctg
     8221 gattcccaat tgccaaaacc tttgacgtat tcatacccaa gaacaatcct cacaccactt
     8281 accacttgcc agacaaaatt ttactcttat ttcataaaac tgaatcgggc gtagagtgac
     8341 aggcttcggt aatatgattc aactttggtt ttaaatacta atggctcatc ttttgtaaaa
     8401 ctggtaaaat tatggcaata ttcagcacct acagtaatgt ttttgttaaa atgatactca
     8461 atgccaagat tggcaccggc tgttattgaa ttgtaaacat ttccgccagt atagtcttca
     8521 tagattacca aataattttt attcttccag atatttttat caaccctgcc accggcaagc
     8581 actgaaaatt tatcatttat cctgtatcca atgttgacag ggaaagacaa catatctgaa
     8641 atctggccac gaacaactcc tggaatagta tcatattttg catcaggttc tctgtatcct
     8701 cgctgagtcc attcgagacc cgtaacgagc ttaactccat tgtcagcaaa ttgatattca
     8761 agaccaaagc caatagtggg gcgaattaac ggagtataaa agtgatcgat tctcttataa
     8821 gtaccatcca atttttcaac ttttgaattt acgacacttc ctactgcatc ggctttaaag
     8881 taagttttaa tctgcgcatt tgctacatgg gtactgagga taaataaaac aaataataat
     8941 aatatttttt tcatcagaaa gtgtaattta agttaaattc aaagccttga ggacatctgc
     9001 taataacagt attttttaca ccaaaaaaaa atggatcgtt tgtatctttc actgaataag
     9061 gtgtaaaagc cctttcgaaa tgcaagccaa tagcccaact ttttgtcaat ctgtattctc
     9121 ctccaaaact ccacagaaaa aaataatttt tatatttcgt tctactgcca agacctttga
     9181 atgctgtatg cggctcacca agtgatggca aacttgtgtt agaagtgata gccaaatcat
     9241 aatttatacc tgctactaat gataatcttg aatttttcgg agtaaacaca atttctgaag
     9301 tagctcccaa acatttatgc caagaattaa aaataacgtc ggggtaataa ccatcacctt
     9361 tggggcttga tctttcaatg aaccggactc ccaaccgtac cttccagata tcattaaatc
     9421 catatccgag cttgccacct atctggtatt taagcccaaa tagcggacct tcgctacccg
     9481 tatatacagg ctggttataa aaaaatagtg tagaaatgca agagtaattt ggtcctgcat
     9541 aaactgtata atcaagacgt gactgtgcaa ccacacaatg aaacatcaca aaatacagga
     9601 agcttaataa aaagattttt cttctcctcc aatttttaat ttaattaaag attcacaatc
     9661 aaacttaaat tcggaaacaa ttaatttttt atcgattgca tcgtgtttct aaggctatat
     9721 atggagattt cgtcctgcta ccagaaaatc gtacatactt caaacagaac tcgggttcaa
     9781 aatccaccaa tgctaaaacg ggttgatttg caaaaccgta acgacgttca taggttgctc
     9841 ttgctttatc agttacgcca taaactgaag caatataagg gttgggtgta atattacaag
     9901 cgtgcgtaat cttgaaatca atcttataat tattatagag tttagcacca taccaaaaac
     9961 ctaaaaatcc tttcttctca tttttaattt gaaaaacaaa atcataagcc ggatatttcc
    10021 aataatagcc agaactaaat gggtctgaaa aataagtaaa tcttggttct ataaaatatt
    10081 gtatgtttaa tctgcttttg tcaactgttt tcgaatcagt aaaaagcaat ggacacccat
    10141 catgacaatt accacctctt acaataattt cagttgcaaa gacgccatat aaagtatctg
    10201 tttccaatgt tgtcattgca ttaagcattt ttgaatcatc accatccaat acctgcaaca
    10261 ggtaaccatt gtattcctta caaagggaag tacctatttt aaattcgcca ttcatatttg
    10321 caaattctaa atttgtaaga aacttatttg taaaacttga atcttcagct tgaaattgta
    10381 ccttgttgtt aaaagtagaa atgacataca attcctctat tgaatcagtt gccatttcca
    10441 ttaaattaca aaattcctcc taagcaatgt taggcgtttc aatactcaac gaacttctgt
    10501 attgctccaa ttccaaagaa tcagctaaat taataaaatt cagcgtttga atataatcag
    10561 caaatgtatt aaaacaaatt cttccattat tgatagtaaa attgccaatt tcatttgtgg
    10621 tacgattacc tacaaaaggg ttaggtattg tgttatcaac tacgttggat ttattcgttt
    10681 gaatgtcatc ctttttacag ctacttaaca gtaaaataat cgataggata aatgttatga
    10741 agatataatc tttaacatga aatcctgttc tgttctgttc tgttctgcgc tttttcataa
    10801 tttataaaat ttaatgggtt aaaaaacaat acaaaacatt aaaaaatcga aactgtacct
    10861 attcttgtct attgtgtgat atttgattgc ctaaaagtac tttgttagct ccggcgctgt
    10921 aagttttttc aacacttttt attaaaaaaa attaaacacc aaataaatga tattatttta
    10981 ttgaaaatca tccttttaat gctgcactca ggcctgaaac ggtacatgga ggtatgttac
    11041 accatcacaa tcttggtaca catctcc
//
LOCUS       DAOQQY010000061        11055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2123_length_11055_cov_5.326091, whole genome shotgun sequence.
ACCESSION   DAOQQY010000061 DAOQQY010000000
VERSION     DAOQQY010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2123_length_11055_cov_5.326091"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PLE23_04715"
     CDS             <1..496
                     /locus_tag="PLE23_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMX82600.1"
                     /translation="FHVPVIAAVNGFALGGGFELALACHLRIAGEKAKFGLPEVNLGL
                     VPGYAGTQRLHQLIGKAKALEMLMTTDMIGAEEATKLGIANHAVAAGEEVPKAISIIE
                     KIATKGPKAVAKVIELTNIFYNGTENGFSAEQRIFGSLFRQPESIEGINAFLEKRTPE
                     FRKS"
     gene            573..1580
                     /locus_tag="PLE23_04720"
     CDS             573..1580
                     /locus_tag="PLE23_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMX82601.1"
                     /translation="MIETDIIIIGAGPAGLFTVFEAGLLKLRCHLIDYLPQPGGQLSE
                     IYPKKPIYDIPGFPEVLAGELVDNLMEQIQPFKPGFTLGERADIIEKNEDGSFTVITS
                     RGTRHKAPVIAIAGGLGCFEPRKPPIDNIAEFEDKGVEYIIKDPEIYADKTVLIAGGG
                     DSALDWTIYLADIAEKVIMVHRRNDFRGAPDSVDKVKYLAQQGVVELITESQVTGLGG
                     NGRLRTVNIEHDKLGTLTRDVDHFIPLFGLTPKLGPIANWGLNIDKQAIEVNTFDYST
                     NVPGIFAIGDINTYDGKLKLILCGFHEATLMVQSAFKIIHPDQKLSFKYTTVTGIEAR
                     L"
     gene            1577..1900
                     /locus_tag="PLE23_04725"
     CDS             1577..1900
                     /locus_tag="PLE23_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMX82602.1"
                     /translation="MIEITVIDRKGQEHLLEAPTDMNLNMMELCKAYELPVKGTCGGM
                     ALCSTCHVYVLSDQQLPEMTADEEAILDTAFFVKSNSRLGCQLKITDNMNGLRVQLAP
                     EDEEN"
     gene            complement(1923..2261)
                     /locus_tag="PLE23_04730"
     CDS             complement(1923..2261)
                     /locus_tag="PLE23_04730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HMX82603.1"
                     /translation="MSDFPPCPQCKSTYTYQMDELFVCPDCAHEWNPAEEPAEESGLV
                     VKDANGNILQNGDSVIVVKNLPVKGMAQGIKAGTKVRNIRLVDGDHNIDCKIDGFGAM
                     GLKSEFVKKA"
     gene            2466..4544
                     /locus_tag="PLE23_04735"
     CDS             2466..4544
                     /locus_tag="PLE23_04735"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HMX82604.1"
                     /translation="MHKQIRWRRYPFVRLLLPFVAGVVLADISFFPNYYYSLILFLLT
                     LAGVFLFQFFKRRPSAAWPDGILLYFLFFLLGIFTIRIHDPGLSDQYFKHFTPLQKAS
                     LVIRIDDLDPSDNWFKINGTVIRTLDSNKVWHLSNGKILLFAARDSATLLPGDVVCVS
                     GKLMEVPPPLNPEAFDYRKYLFRKDVLYEIFARKDEWTVTGYKDINLSILASNLRLRL
                     VALIKEYVKPEGENSVAAALLLGYRNDLSDELNNAYINTGSIHILAVSGLHVGLVSGI
                     LMLFLNFFKKNNLTVRLIKLTVILGFIWFFVLLTGAGASVVRAAVMFSFIHTGQLLLR
                     RKYIYNTIAASVFVILIWCPQMLFDVGFHLSLSAVLGIIALQPFLLQLYHSENKIIHG
                     AWELTTVTIAAQLATLPIALYYFHQTSLYFLISGIFVVPISSFALYAGVGLAIFHWIV
                     PVLAKLAGTIMYWSVFAMNSIIYAIAGMPYNLITDIPFNTWEFLLLSTTVVFVTVYVS
                     LRRKALLYMALAAFLIFSITRIAFNYAHSAQQILIFYNTKENVFVDYFNGNSCISLRE
                     NTDQREEKYVASGARMAYRILETTNIHTGQSYSGIYNSDEGLLSVRNYRLKFLFKNSE
                     NFYGIGEGTSVYVCEDVRSEDCVKDKIPQMVFLGSKIDFRTRKNWKKFCADNNIRCID
                     LKSESYVLKL"
     gene            4547..5716
                     /locus_tag="PLE23_04740"
     CDS             4547..5716
                     /locus_tag="PLE23_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMX82605.1"
                     /translation="MDNNLRAYFYFTKNERIGILVVCFLSFCLLFVPKFFQQSGFLAK
                     PTTSNELQDADNRALEHIHAKSSNTRSAEREVTEHEYKYSGKLDVNTASFKQLKSIGI
                     SSKTAAIIIKYRDKGGQFRNLDSFKKIYGITANELNSIREKVLFPEQDASATGNAVLK
                     PFDFDPNSVSKEELAAMGLPLRIIQTISNFRDKGGRFYEKNDLAKIYGMKPEWIESLL
                     PFVSISANESPRTTFESAFAEERQSNVKPKNYRTEVHKSEVFNINTATKEDLMKIKGV
                     GPFYAEKIIKFRDALGGFVTVAQLAETRGLPDTLVKSIAPQLTVNGDVNKLKINQLNK
                     SDLARHPYISHPQALILSNYIQQHGKMQSLEDVRKSRAFEPAELNKLVPYLDFSE"
     gene            complement(5782..6435)
                     /locus_tag="PLE23_04745"
     CDS             complement(5782..6435)
                     /locus_tag="PLE23_04745"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HMX82606.1"
                     /translation="MEQQTIPYSYIAIEGNIGAGKTTLATMLAEQFDCRLILEQFTDN
                     PFLPYFYNEPDRYAFPLELFFMTERYKQLQSMLPQQELFSDLTISDYFYTKTLLFARN
                     NLKDEEYRMFQKMFHTLSSAFPKPQIILYLHRKVDNLLDNIQKRGRQFEAPITSEYLK
                     KIQDVYFDYFRVETSIPIVILDVTNVDFINNIRFFNEVANCLSKLYRPGIHHLTISL"
     gene            complement(6437..6931)
                     /gene="folK"
                     /locus_tag="PLE23_04750"
     CDS             complement(6437..6931)
                     /gene="folK"
                     /locus_tag="PLE23_04750"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HMX82607.1"
                     /translation="MMTRTKVFLLLGSNIGDKKQQLENAVKLIHKECGLVKKKSSIYE
                     TEPWKVEGQEVYYNMAIEIETTFTPDLLLARIKRIEKELGREKREKWAPREIDIDILF
                     FGRKVIKQKDLQIPHPHMHERNFAILPMMEIAPEFKHPLLHHNMEELYLRSKDNYEVV
                     ILGK"
     gene            7071..8000
                     /locus_tag="PLE23_04755"
     CDS             7071..8000
                     /locus_tag="PLE23_04755"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX82608.1"
                     /translation="MTNNRIHYLETEKHKADIHVEIKRPKGAGLLQFGFSVMQKIAPK
                     WSAEKAFSLFITPMRRAKHSFEDELLKEVIRHETIFDNKKIRVYEWKGGDKKAVLAHG
                     WESRATALRTVVPQLRERGFTVYAIDAPAHGESEGKQTNVIEYAHLIGQVAREFGGFD
                     IAVAHSFGGLALSYALVEIPGFMVPNVIMAGLPASTKLALQGMYRFLHLERPVQELIE
                     KKIYESTNYSADELSVAHLSTKFQNVNGLLFHDEKDNLVPLYTAIDSVNAWQSSTLYV
                     THGLGHFRVIKSKEVLMTMFDWVDHHFSHPKAF"
     gene            complement(7951..9567)
                     /locus_tag="PLE23_04760"
     CDS             complement(7951..9567)
                     /locus_tag="PLE23_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HMX82609.1"
                     /translation="MFRRITASIAGLLFVGNIYAAGIDETIDKAVKPASDFIEKYVFY
                     PIPLPGDNAMPLVLCFLIFAAVYCTIYFGFPNIRYFKHAISTLRGKYKNIESIKHPIY
                     TSSLVNIVDEDIVDTIRDESVQGEVSHFQALSSALSGTIGLGNIAGVAIAIAIGGPGA
                     TVWMIISGFLGMSSKFTECTLGTKYRDIGPDGTVYGGPMYYLSKGLAEKGYKTIGKIL
                     AVVFAIFCVGGSLGGGNMFQSNQSAAIVKDIFKLDSGNTGFFIGIFLAILTGVVIIGG
                     IKRIGSVAEKIVPFMCGIYIIAGLVVIGINYSYVPAAFGAIFEGALSAQSIAGGVIGV
                     LIAGFRRAAFSNEAGVGSAAIAHSAVRTRYPASEGIVALLEPFIDTIIVCTMTALIIV
                     ISNTNHHFLEYGSGTGLNGVALTSAAFETTLPGFSYILALAVFLFAFSTMISWSYYGL
                     QAWLYLFGRTKSNELSYKVIFLIFVVIGGSIQLNSVIGISDSMILAMVFPNLIGLLVL
                     SPVVKEEVSRYLAAIKMLLGGKSDGQPNRT"
     gene            complement(9775..10434)
                     /locus_tag="PLE23_04765"
     CDS             complement(9775..10434)
                     /locus_tag="PLE23_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HMX82610.1"
                     /translation="MKLHFLFIISLIAFISCKEGAKTNTATSTPTDNSVQGSGDMKDF
                     EIADIPGSNYKRATKKDENGHTIEEGFFLDGKKTGTWFNYYPDGRVVAIRSFIDGKVD
                     GTFITIDDRGRILLQALYRDNFLNGVVTEYKNGSRRMKETFYKDGKKNGVQREYFELG
                     PPRKEVDYKDDVVDGKVKFFNDKSVLIAEYEYKNGQKVGGGVVSGADSTGLNIALPEG
                     K"
     gene            10540..>11055
                     /locus_tag="PLE23_04770"
     CDS             10540..>11055
                     /locus_tag="PLE23_04770"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMX82611.1"
                     /translation="MNNKEIAKLFSELADLMELQNENPFKIKSYRNAHVSLRKIDTPL
                     NTLSQTEMEVLPGVGKAIAEKITQIVSTGKMPTLEKYRAIVPEGVREMLKVHGLGPKK
                     IDVIWRKLGIESPGELLYACDENRLVELPGFGTKTQEDVKSKLAFYFNASGKYLYPKV
                     SETLQTILDELN"
BASE COUNT         3301 a         2265 c         2129 g         3360 t
ORIGIN      
        1 attccacgtg ccggttatag cagccgtcaa tggttttgca ctcggcggag gattcgaact
       61 tgcccttgcc tgtcaccttc gtatagctgg cgaaaaagcc aagtttggcc tgccggaggt
      121 taatctcggg ttggtgcccg gttatgctgg tacacaacga cttcatcagt tgattggcaa
      181 ggcaaaagct cttgaaatgc tgatgactac cgatatgata ggagctgagg aagccactaa
      241 gctcggaata gcaaatcatg ctgttgctgc cggtgaagaa gtaccgaaag ctatttcaat
      301 cattgaaaaa attgctacca aaggccctaa ggcggtcgct aaagttatcg aacttacgaa
      361 tatcttctat aatggcaccg aaaatggctt ctctgccgag caaaggattt ttgggtcact
      421 gttccgccaa ccggaatcaa tagaaggtat caatgcattt cttgaaaaaa gaacacccga
      481 attcagaaaa tcataattgt tactgattat agtcatgaac tttgatcgtt attgatgtgt
      541 tatctttgcg attgaatgat taatgtgtta caatgatcga aacagatatt attattattg
      601 gtgccggtcc ggcaggatta tttactgttt ttgaagcagg tttgctcaaa ctgcgttgcc
      661 accttattga ttatttgcct cagccgggtg gccaactttc tgaaatttat cctaaaaaac
      721 caatctatga tatacctggt tttccggaag tactcgccgg cgaactcgta gataacctga
      781 tggaacaaat acaaccattc aaaccgggct ttacgcttgg tgaaagggca gacatcattg
      841 aaaaaaacga agatggcagt tttacggtta tcacaagccg cggtacccga cataaagctc
      901 ccgttattgc tattgccggt ggcctgggtt gttttgagcc tcgcaaacca ccaattgaca
      961 atatcgctga gttcgaagat aaaggtgttg aatatatcat caaagatcct gaaatatatg
     1021 ccgataaaac tgtactgata gccggtggcg gtgactctgc tctcgactgg actatctacc
     1081 tggctgatat tgccgaaaaa gtgataatgg tacacagaag aaacgatttt cgcggtgcac
     1141 cggattctgt cgataaggtg aaataccttg ctcaacaagg tgtcgtcgaa cttataactg
     1201 agtctcaggt gacaggctta ggcggaaatg gaagactccg tacggttaac atcgagcatg
     1261 acaagctcgg cacactcacc agagatgttg atcactttat tccactgttc ggcctaacac
     1321 caaaactcgg cccaattgct aactggggcc tcaatattga taaacaagct atcgaagtca
     1381 acacttttga ctattcaacc aatgtccccg gcatttttgc tattggcgat attaatacct
     1441 atgatggcaa actcaaactc attctttgtg gctttcacga agctacattg atggtgcaga
     1501 gtgcttttaa aatcattcac ccagatcaaa aactttcttt caaatacact acagtcacag
     1561 gtatagaagc cagattatga tagaaataac cgttattgac agaaaagggc aggaacacct
     1621 gctggaagca cctaccgata tgaacctcaa catgatggag ctatgtaagg cgtacgaact
     1681 cccggtaaaa ggtacatgcg gtggcatggc actatgttct acttgccatg tttatgttct
     1741 gtctgaccag caattgcctg aaatgaccgc cgatgaagaa gccattctcg atacagcctt
     1801 tttcgttaag tcgaacagcc gtctaggttg tcagctaaaa attacagata atatgaatgg
     1861 tctccgtgta caactagcac ccgaagacga agaaaactaa tagaaacaaa gttttgttag
     1921 tattatgctt ttttgacaaa ttctgatttt aagcccatcg ctccgaatcc gtctattttg
     1981 cagtcgatgt tgtggtcacc atccactagc ctgatattcc taaccttggt tccggctttt
     2041 atgccttgtg ccattccttt taccggcagg tttttgacta cgatcaccga gtcaccgttt
     2101 tgaaggatat taccattggc atcttttact accaggcctg attcttccgc aggttcttct
     2161 gccggattcc attcatgggc gcagtcagga caaacaaata attcgtccat ttggtaagta
     2221 taagtggatt tgcactgtgg acaaggtgga aaatctgaca tattgttctt tctattaatt
     2281 cttaatgtag caaagttagg cttttttaca taatttttag cccttataat acaaattacc
     2341 gcatgtggcc tttatcggaa tataatttca cttatgtagc tgaaaaggct gccaattgaa
     2401 atttttttcc aacaatggat acttcgattt agtttaatac atatttttgt cataacagca
     2461 cacttatgca taaacaaatc agatggcgcc ggtatccctt tgtgcgatta ttgcttccat
     2521 ttgttgcagg tgtcgtactt gctgatatct ctttctttcc caattattat tattcactca
     2581 ttttatttct acttacttta gcaggagttt ttttatttca attttttaaa agaagacctt
     2641 ccgcagcttg gccagatggc attttgttgt actttttatt ttttctgctt ggtatattta
     2701 caattcgaat acacgaccct ggcttatctg atcaatattt taaacatttt accccactac
     2761 aaaaagccag ccttgttatc cgtattgatg accttgatcc ttccgataat tggttcaaaa
     2821 ttaatggcac cgtcattcgt acacttgact ccaataaagt atggcatcta agcaacggga
     2881 aaatcctgct atttgctgcc cgcgatagtg ctactctctt gcctggtgat gttgtctgtg
     2941 tatccggcaa gcttatggag gtgccaccac ccttgaatcc agaagctttt gactatagaa
     3001 aatacctgtt ccgaaaggat gtattgtatg aaatatttgc cagaaaagat gaatggacag
     3061 ttaccgggta taaggatata aatctgtcaa tccttgcttc aaatctccgt ttgcggctag
     3121 tggctctcat taaagaatat gtaaaacccg aaggggaaaa ttctgtagct gccgcgttgt
     3181 tactcggcta ccgcaatgac ttgagcgacg aactcaataa cgcttacatc aacaccggat
     3241 ctatccacat tcttgctgtt tcgggtctgc atgttggtct ggtaagtggt attttgatgc
     3301 tttttctgaa tttttttaag aaaaataatt tgaccgttcg cttgataaaa cttactgtaa
     3361 tactcggctt catctggttt tttgtgctgc ttacaggtgc aggtgcttct gttgtgagag
     3421 cagctgttat gtttagtttt attcataccg gacagttgtt gcttcgtcgg aaatatattt
     3481 ataacacgat agctgcgtct gtgtttgtta tattaatatg gtgtcctcaa atgttgtttg
     3541 atgtcgggtt ccatctttct ctttcagcag tgcttggtat catagcgctt cagccatttt
     3601 tactgcaact ttaccattcc gaaaataaaa ttatacacgg agcttgggaa ttgacaactg
     3661 tcaccattgc tgcacaactg gcaacactgc ccattgcact ttactatttt caccaaacga
     3721 gcctttattt tctcatttca ggcatttttg tagtacccat ttcttctttt gcgctatacg
     3781 ctggtgtagg tttagcaata tttcactgga ttgttccagt gttggccaaa ttggcaggaa
     3841 ccatcatgta ttggtctgtt ttcgcaatga attctatcat atacgccatc gcaggaatgc
     3901 catataatct catcacagat attccattca atacttggga attcctttta ttatctacaa
     3961 ctgttgtctt tgttacggtt tatgtttcat tgagacgtaa agctttactt tatatggcat
     4021 tggcagcttt tttgatattt agcatcacac ggattgcttt taattatgct cattcagccc
     4081 agcagattct tatattttat aatacaaagg aaaatgtgtt tgttgattat tttaatggaa
     4141 attcatgtat cagcctcaga gaaaatacag accaacgtga agagaaatat gttgcgtccg
     4201 gagctcgaat ggcttaccgt attcttgaaa cgaccaacat acataccggc caatcttatt
     4261 cgggtattta taattccgat gaaggcttgt taagtgtcag aaattatagg ttgaaatttt
     4321 tatttaaaaa ttctgaaaac ttttatggaa tcggagaggg tacaagcgtc tatgtatgcg
     4381 aggatgttag aagtgaagat tgtgtaaaag ataaaattcc gcaaatggtt tttctaggta
     4441 gtaaaataga ctttagaacc agaaaaaact ggaaaaagtt ttgtgctgac aataacatca
     4501 ggtgtattga tttgaaatca gaatcttacg ttttaaaact ttgattatgg acaacaatct
     4561 gagagcgtat ttttatttta cgaaaaatga acgaattggt attctcgttg tgtgttttct
     4621 ttcgttttgt ttgctttttg taccgaaatt tttccaacag tcaggattct tggccaaacc
     4681 aacaaccagt aatgaattgc aggatgcgga caatcgtgca ttagaacata ttcatgcaaa
     4741 atcatcaaac acaaggagtg ccgaacggga ggtaaccgaa catgaataca aatattcagg
     4801 aaagttggat gtaaatactg cttcatttaa acaactgaaa tcaataggta ttagctcaaa
     4861 aaccgcagct atcatcatca aataccgcga caaaggcgga caattccgca atctggattc
     4921 atttaagaaa atttatggta ttaccgcaaa tgaattgaac agcattagag agaaagtcct
     4981 ttttcctgaa caggacgctt cagccactgg taatgcggtg ctcaaacctt tcgactttga
     5041 tccaaattca gtatcaaagg aagaactggc agccatgggt ttacctctga gaattattca
     5101 aacgatttcc aattttagag ataaaggcgg aagattctat gaaaagaacg accttgcaaa
     5161 aatctacgga atgaaacccg aatggattga atcacttttg ccttttgtaa gcataagtgc
     5221 aaatgaatca ccccgtacta cgtttgaatc agcattcgcc gaagaacgcc aaagcaatgt
     5281 gaagccgaaa aattaccgta cagaagttca taaatctgaa gtatttaata ttaatactgc
     5341 aacaaaggaa gatttgatga aaataaaagg agtaggacca ttttatgctg agaagattat
     5401 aaaattccgc gatgccttgg gtggctttgt gacggtagca caattagccg aaacccgtgg
     5461 tttgcctgat accttggtta agtcgatcgc accccagctt actgtaaatg gtgatgtcaa
     5521 caagttgaaa attaatcagt taaataaatc ggatcttgcc aggcacccgt acataagtca
     5581 tccgcaagca ttgatattga gcaactatat tcaacaacat ggcaaaatgc agagcctgga
     5641 agatgttcgc aaaagcagag cctttgaacc ggcagagctt aataagctcg tcccttatct
     5701 tgattttagt gaatgagcta atgtagcttg ctcgaatgcc gtattttctc aactttaacc
     5761 aaatgcggat tctgggttaa atcataagct aatcgttaaa tgatgtatgc caggccggta
     5821 tagcttactt aaacaatttg ctacttcatt gaaaaacctg atattgttta tgaagtcaac
     5881 attagttaca tcaagtatca cgatcgggat gcttgtttct accctgaaat agtcaaaata
     5941 aacatcctgt attttcttca gatattcaga tgtgatcggc gcttcaaact gtcggcctct
     6001 tttctgaatg ttatcgagca gattatcaac ttttctgtgc aggtaaagta ttatttgcgg
     6061 cttagggaag gcagaactca gtgtatggaa catcttttgg aacatcctgt attcttcatc
     6121 ctttaggttg ttgcgagcaa acaaaagtgt ttttgtgtaa aaataatctg aaatggtgag
     6181 gtcactgaat aattcctgtt gtggcaacat gctctgaagc tgtttgtacc gctcagtcat
     6241 aaagaaaagt tcaagcggaa aagcataacg atcgggttca ttatagaaat aaggaagaaa
     6301 tgggttatct gtaaattgct caagtatcag acggcaatca aattgttcgg caagcattgt
     6361 tgccaatgta gttttgccgg ctccgatgtt accttcaatt gcaatataac tgtaagggat
     6421 tgtctgttgt tccatactat ttacccaaaa tgactacttc ataattatct tttgacctca
     6481 gatacagttc ttccatattg tggtgtagca acggatgctt gaattccgga gctatttcca
     6541 tcattggcaa tatagcaaag ttgcgctcgt gcatgtgcgg atggggtatc tgcaaatctt
     6601 tctgcttgat gaccttgcga ccaaagaaca aaatgtcgat gtctatctca cgtggtgccc
     6661 atttttccct tttctctcta ccaagttcct tctctatcct tttgattcgt gctaatagta
     6721 aatctggtgt aaaggtggtt tcgatttcaa ttgccatgtt gtaatagact tcctgacctt
     6781 ctaccttcca tggttcggtc tcgtatattg aagatttctt ctttaccaac ccacattctt
     6841 tgtggataag ttttacagca ttctctagtt gttgtttttt gtctcctata ttgctaccca
     6901 acagcaagaa gactttggtc ctcgtcatca ttgcgtaaaa ttagtgaaat tgtaaagatt
     6961 ttcggtaagt tttcttcaga aattatgtga ataattaaca ctccaactat tttctactta
     7021 tatcaaagta aaggccttaa ctttgagcca tcaataatta gagcaacctg atgaccaata
     7081 acagaattca ttaccttgaa actgaaaagc acaaagcaga tatacatgtt gaaataaaaa
     7141 gaccaaaagg tgcaggattg cttcagtttg ggttttctgt catgcagaaa attgctccaa
     7201 aatggtccgc tgaaaaggct ttctctttgt tcattacacc tatgcggaga gcaaaacata
     7261 gcttcgaaga tgaattgttg aaggaagtta tcaggcacga aacgatattt gacaataaaa
     7321 aaatacgtgt ttatgaatgg aagggcggcg ataaaaaggc cgttttggcc catggctggg
     7381 aatcaagggc gaccgcactg agaactgtag tgccacaact acgcgaacgt ggttttacag
     7441 tttatgccat tgatgcgccg gcacatggtg aatcggaagg caagcaaacc aacgtaatcg
     7501 aatatgcaca tttaattggg caggtagcca gagagttcgg aggatttgat atagcagtgg
     7561 cacactcatt tggtggcctg gctcttagtt atgctcttgt cgaaattccc ggattcatgg
     7621 ttccaaatgt tataatggca gggcttccag cctctaccaa actcgcactc caaggcatgt
     7681 accgtttttt acacctcgaa cgtcctgtcc aggaattgat tgagaaaaag atctacgaat
     7741 caacaaatta ctccgcagac gaactgagcg tagcacatct ttcaacaaaa ttccaaaatg
     7801 taaatgggct tttattccat gatgaaaaag ataatctggt gccactctat acagctattg
     7861 attcagttaa tgcctggcag tcatcaacgc tttatgtgac acacgggctg ggtcatttca
     7921 gggttatcaa atcgaaagaa gtactcatga ctatgttcga ttgggttgac catcactttt
     7981 cccacccaaa agcattttga tagctgctaa atatctactt acttcttctt tcaccaccgg
     8041 agataataca agcaaaccaa taaggttagg gaagaccata gccagtatca ttgaatcgga
     8101 gatgccgata acactgttga gctgaatact gcccccaatc accacaaata tcagaaatat
     8161 taccttgtat gagagttcat tgctttttgt cctgccaaat aaatacaacc acgcctgaag
     8221 cccgtaatag ctccagctaa tcatggtaga aaatgcaaat aaaaatacag ccagagccag
     8281 tatataagaa aaacccggta acgtagtttc aaaagcagcc gatgtaagcg ccacgccatt
     8341 caatccggtg ccggaaccat actccagaaa atgatgattg gtattgctaa tgacaatgat
     8401 taatgcagtc atggtgcata ctattatggt atcaataaat ggttcgagca atgctacaat
     8461 gccttcagat gcaggatatc tggtgcgtac tgcagagtgg gcaatggccg ccgaacccac
     8521 gccggcttca ttcgaaaatg ccgcccgcct aaaccccgca attaatactc caatgacacc
     8581 tcctgctatc gattgggcag acaatgcacc ctcgaatata gctccaaatg cagcaggtac
     8641 ataactgtag ttaatcccaa taaccacaag gccagcaata atatatatac cacacatgaa
     8701 tggtactatt ttttcagcca cagaacctat gcgttttatt ccaccaatga taaccacacc
     8761 tgtcaaaata gccagaaaaa taccaataaa gaaacccgta tttccggaat cgagcttaaa
     8821 aatgtctttg acaattgccg ccgattggtt agactgaaac atgttgccgc caccaagtga
     8881 accaccaaca caaaatatag cgaatacaac agctaatatt ttaccaatgg tcttatatcc
     8941 tttctctgct aagccttttg ataaataata catcggccct ccatatacgg taccgtcagg
     9001 acctatatca cggtattttg tgcccagggt acattcagtg aatttcgatg acatgcccag
     9061 aaaacctgat atgatcatcc atacggttgc tccgggtcct ccaatagcta ttgcaatggc
     9121 tactcctgct atgttaccta agccaatggt acctgaaagt gcactcgata atgcttgaaa
     9181 atgactaact tctccttgca ccgattcgtc tctgatggta tctacaatgt cttcatccac
     9241 tatatttacc aaactacttg tgtatatcgg atgctttata ctttcaatgt ttttatactt
     9301 tccacgcaag gtgctaatgg catgtttaaa gtatcttata ttaggaaaac caaagtaaat
     9361 tgtacaatat acagccgcaa aaatcaaaaa acacagtaca agtggcatcg cattatcccc
     9421 gggtaaggga atagggtaga atacgtactt ttcaataaaa tctgatgcag gttttactgc
     9481 cttgtcgatc gtctcgtcaa ttccggcagc ataaatattt ccaacaaata ataacccggc
     9541 aattgaagct gttatgcgtc tgaacatact ttgatatttt ggctaaaagt aaatatattt
     9601 ttttctttct tgccaaatat taagtaaaaa ttcaaatcat cgataaaaaa taattttgta
     9661 attatttgtc aatcagtatt atgtgagaat agcttttctg ctctatatca acgatttgtt
     9721 ttctacaaag cctaagatgt gaaagatcca attctcaatt gggttctata aggtttattt
     9781 cccttcaggt aatgcaatgt tcaagcctgt cgaatcagct ccgctaacaa caccgcctcc
     9841 tactttttga ccattcttat attcatactc ggcaattagt acgctcttat cattgaaaaa
     9901 ttttacttta ccatcgacta catcatcctt ataatcaacc tcttttcttg gtgggccaag
     9961 ttcaaaatat tcacgctgca caccgttctt ttttccatcc ttataaaatg tctccttcat
    10021 tcggcgacta ccgtttttat attctgtgac cacaccgttc agaaaattgt cgcgataaag
    10081 tgcttgaagc aatatccgac cgcggtcatc aatggtaata aatgttccgt ccacttttcc
    10141 atcaataaaa ctcctgatgg ctacaacacg cccgtccgga taatagttaa accatgtacc
    10201 tgtctttttt ccatcaagaa aaaatccttc ctcaatggta tgcccgtttt cgtctttctt
    10261 agtcgctctt ttgtaattgc taccgggaat gtctgctatc tcaaaatcct tcatatcacc
    10321 cgatccctgt accgaattgt cggttggtgt ggatgtggca gtatttgttt tggctccttc
    10381 cttacatgaa atgaaggcta ttaagctgat tataaagaga aaatgcaatt tcatagttct
    10441 tatattttaa tgtgtacttt acgatttcaa ataaaaataa gtttcaaccg atggtaaaat
    10501 gatttgttta tctatcttta gataataatc ttcggacaaa tgaataacaa agaaatagca
    10561 aaacttttct ccgaacttgc agacctaatg gaattgcaga atgagaaccc gttcaaaata
    10621 aaatcataca gaaacgctca tgtgagcctt cgcaaaattg atacgcccct aaatacgctt
    10681 tcgcaaacag aaatggaagt attgccggga gttggtaagg ctatcgctga aaaaattacc
    10741 caaatagtga gtaccggcaa gatgcctaca ctcgaaaaat atagagcaat agtgcctgaa
    10801 ggtgttcggg aaatgctgaa agtacacggc ctgggtccca aaaagataga tgtcatctgg
    10861 cgaaaacttg ggatagaatc tcccggcgaa ttactttacg catgtgatga aaaccgactc
    10921 gtcgaactcc cgggctttgg tacaaaaaca caagaagatg taaaatcaaa acttgcattt
    10981 tactttaatg cctccggcaa atatctttat ccgaaagtat cagaaacact tcaaactata
    11041 ctggacgaac tcaat
//
LOCUS       DAOQQY010000062        10971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2156_length_10971_cov_6.779864, whole genome shotgun sequence.
ACCESSION   DAOQQY010000062 DAOQQY010000000
VERSION     DAOQQY010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10971
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2156_length_10971_cov_6.779864"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            462..4112
                     /locus_tag="PLE23_04775"
     CDS             462..4112
                     /locus_tag="PLE23_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82612.1"
                     /translation="MFVSFFLLPILSSYTVAIGGKVDDLGVKTAIGCDFYIYNIHKLA
                     NGDYEFCVKTLNPGSFDNFLLWDQSVLVIQTTDPNFCYTTSSPYISIQQTNIDSTYCT
                     LSTVFTLDEECPRLSNITVDNCTKNISFDLNLNYKAYVDFGDGSAIEVSDWLGALNVS
                     HQYSNNVLYNVCITYDNGETMQYCCYPIDLSMCTNSEFTPVIDTLSTSCLKPLYHEIP
                     YCGPSSCVSIWEYEDGTVYYGHTRPSHYFTNFVNADDSVWIKHTVICCNDTSVTVKKI
                     PHKNAAFIGTPGVITNLSDTIQWSDGPDPLTATFYKMTVNDFILAYSNNPNLPLLIDG
                     TLNVDINASFGTGQWNMGKGSEIVVEGKGLRLSNVNIWSAVNLGVGYAACCDNEYTWF
                     GIRSEGISRIIVSECTISNALVALHYPGLSVTNSNFPKLSVINTSLLGNMFALRNENT
                     PLNITGFSGNFIRGRQDCICTCDVVNALDFRGKNLAVVFPGGETNEILQYEQGLHSEN
                     TSVTLRNFYIHDLDNYAPITPWPNPSDDYSKIGVYFQNTGDFEGNSALTLENCNFNAI
                     PNNDEVMRVGVKVSLEAGGHTLTAIGDTVTESIIMKKTNNGYAINVENGAQIRGEISA
                     NKIYTSDPVNSEGISLEMRSQNNQLNIQNNIIDVNGGTSLSMGIKLGSLNEAVQKIRA
                     IGNKVSLASNTAEGNAIRVDNCEEVVVKDNFIYSNSETPCIEVTQGGNSDINCNYVEG
                     GRIGLKIDATWDNNIISNSFFNNFQNGLYANGKCIDTKGSKIALNSFDYNYNESIYYD
                     IMCNTGRQWHDGYNMFYNQNGDNEVIHIGDEEFVKMCAFHINPASVLGSVTYPDHDPS
                     GIILNKTVKPYTHIVFSCSPGIDEPGNKLYMDNVSIYERYAGILSDTALALSVTAAEY
                     GHLKQSVMDLIARNPGWLLSNSVLSDFYTAEQSAFSGKSAEIIGLFRAFQQTTGVQQE
                     HLQVLRDSHSVVQTEILSLMATLDTLTNPTAISSTILLIEDLQQQAAQVMNVLDSLRS
                     VHDTLNTTALTALAAANGSLSVSEDYQVWEQDMNDYTIKLLKGEALDSAELSQIRAVA
                     SECYRNGGKAVLDARILAAACLKEYYSVEACAEPPGGAGMSSQAPEKLYIYPNPGRDL
                     LNIRIGVDNDASQAELRVYDLMGRLMHVQETFVNTGMDITIDISRLPEGMYVAELKTS
                     DSSFFEKFIIQR"
     gene            4181..5824
                     /locus_tag="PLE23_04780"
     CDS             4181..5824
                     /locus_tag="PLE23_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82613.1"
                     /translation="MSNFNRKIKYLIILALSVFCIIPTIYSQNSFIGVFSHLTGPAKF
                     NPFQEYYPFMFTHLDIYKTKDKGLLYCYLGAREWDENKSQFINVIKYDSLYTPLWHKI
                     YMTTEEAMRPQIMEDSLGSIFLVYNQHDSMLYKIGISKLNPDGNIIWTKMLLDRSRHS
                     QFRYAMLAQNGDLIISGYRIIEPGNIQEALAMRVNGDGEIVWQKLFGGISRFFNDVTE
                     TKDGSLLFTAWDGAVVKLNAGGAVLWANFYDKNDIRTVLEDKEGNYVVATAPTGSVSV
                     ASKCRVFSISPTGELLWKKDYFFDFWAGEGNGLFGGFISQMKTSLQIVLWTVNNSKMI
                     FLNTDYGGNINWASRLQGDGWFALKDLNQAMDDGILVPFFCNDGLFDYKSKGFSGVIM
                     TDPDGGIPCVEKLYDPPVDVVDIALTAPTPATGVQNASFKFADFSLEVEDAPMVTLEM
                     LCYATAVRPEPGRRAGFSIYPNPATEMLNIRTATDVLASQAELRVYDLMGRLMHVQET
                     FVNTGMDISIDISRLPEGMYVAELKTSDSSFFEKFIIQR"
     gene            5842..7539
                     /locus_tag="PLE23_04785"
     CDS             5842..7539
                     /locus_tag="PLE23_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82614.1"
                     /translation="MKDILRKLFYAFFILTIIVSAEGRLYSQNSFIRIFQDTVYDFGN
                     EVNVSIFEAKDKSIYRTSYYSSYKENGSSVPKITLIRLDSAGQFIWRKSYKIAQKDPN
                     NAKVFCQDKAGNIYLFYHHNITLPFNQYFSKGYVWKLSPDGELIWAKGLENTESTVEL
                     LDGAQYDNDDMLLCGTSFVKYTNGITYDWNPFVMRINTEGEILFQREISYASTPPHSG
                     KFQKIIRTQDHGFVLLGLKNSNNSYCATGVTKIGTYIAKLDSTGTLLWGNQYPCRAPV
                     DIFENNDGTFTVLTQAYNGPPSTSKTFGLFNIDSKGSGKWAREYEFTDTKISAVTVSR
                     MFYRGSSIDIVVNLPKSLMILNLSTDGLINYANTIENQSDPKLNAYDSYGSAMLIEGK
                     GFVMPGSVEYKNPTLIKTNQMGEPPCFFDIVPNPAIENNIVALKLYENNNSKRITSYK
                     FTDDDVIIQSGLDSLSNKLYCLTTAVREQALSQDESFVLYPNPAYQSVTISSRVQDSP
                     AIDGVRLHDIYGNLFRCTSVRITRGVELDLTVIPPGLYIVSLQTSARTIFKKLVVQR"
     gene            complement(7574..8233)
                     /locus_tag="PLE23_04790"
     CDS             complement(7574..8233)
                     /locus_tag="PLE23_04790"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HMX82615.1"
                     /translation="MFGFRIHREGFESIVIAAIISMVLIAAAEYTPVVISALLVIVAG
                     FLFLIVLQFFRNPVRKIPVADNNLVYAPADGKVVVIEEAIETEYFNEKRLMVSIFMSP
                     LNVHVNRNPVSGSIEYAQYHPGKYLVAWHPKSSTENERTTVVYNTGKEKILMRQIAGA
                     LARRIVNYLETGNQVKQGEDMGFIKFGSRVDLYLPLDAKIKVNLEDIVRGNQTVIAEL
                     A"
     gene            complement(8323..9177)
                     /locus_tag="PLE23_04795"
     CDS             complement(8323..9177)
                     /locus_tag="PLE23_04795"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HMX82616.1"
                     /translation="MSKFKSLGARATSATIFAIVMIAGIYLHKVGLVLLFGVIMFIGL
                     RELFTLSFTGDEEGLRKYRIPGTVLAFSPYLWVAVQKLILPKEISSTFVNIAMFLLPV
                     FIAMLLELLGRARQQFSRVAFVALGIAYLTVPVSLVCDLAIQDKGFHPDIIMGLLVLI
                     WISDTGAYFAGSAFGKHKLYPAISPGKTWEGSIGGAVITIITAWFFPELFGEFSAGQW
                     VILGIIAVVFGTLGDLTESMLKRNNGVKDSGNIMPGHGGILDRFDAFIFLIPFAYLYI
                     RLFIYGLY"
     gene            complement(9164..10111)
                     /locus_tag="PLE23_04800"
     CDS             complement(9164..10111)
                     /locus_tag="PLE23_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HMX82617.1"
                     /translation="MTNVDDSTEERNIPVEENEKPHFVKNLVRLAFLGLLGILFLSLS
                     SVWISKVFSVDIQALMSGNIETGNERERSALRLLLVLNSVLAFILPAFIYNMVSDRKY
                     IFNKIGIESTGEQGKKFIWAVGLFILSLPLVMFTAWVNQKIPLPEWASQTEENVSGLL
                     KMLLSEGTTTSLILNIVMLALLPAIGEEWFFRGSLQRIFTQMWGDTHHIKAILLTAFL
                     FSLLHFQFEGFLPRLLLGVVLGFIFYKTGNIWVAVFCHFLFNASQILVAYMTQNDAQQ
                     IDAEKMDSPPLLVVVVCSILFFILYKYSPGIKTDTDEQI"
     gene            10128..10850
                     /locus_tag="PLE23_04805"
     CDS             10128..10850
                     /locus_tag="PLE23_04805"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HMX82618.1"
                     /translation="MTRLSVNINKVALIRNSRGGNLPDLVKIAIDCEKFGAEGITVHP
                     RPDQRHVRFDDIPKLKSVVTTEFNIEGNPTREFMKLVTDNLPDQCTLVPDAPGVLTSD
                     SGWDTVAHYPYLKDITEELHAYNIRVSLFVNAEEKYIEAAAKTGTDRIEIYTGKFSHD
                     FYTDPGQAVQAHIRCAALADHLGMGVNAGHDLNLDNLSYYTKHVKPLHEVSIGHALIS
                     DALYYGLQNTIQMYKRQLIEFQ"
BASE COUNT         3392 a         2100 c         2319 g         3160 t
ORIGIN      
        1 ttatctttct tgaaaaaaat aatgtgagtg cttggttttt accatagaat acgcaattca
       61 tattttattt tccagaagca attcaagtac tgatgattaa tagtacgcca catatatgta
      121 gtatgtcttt actaaatcag gccataaata gaatatcagc aaataatatg taaataacta
      181 tataaataac acttttattc tgcacaatag gttttatttt attgaattca gaataatatt
      241 ttgttacatg aggtttttaa tttatagtac attaaaatag gtcaaaaaat atttcaaaaa
      301 tttttaagac aaaatttgga ttatatgaat aaatgatgta tattagcgga tgttacttca
      361 ctgaataatt tttttattat gagaacacag aacacagaac acagaacaca gaacacagaa
      421 cacagaaatt ttgtcaacat aatgtttgca aatatattgt tatgttcgtt tcctttttcc
      481 tcttacccat attgtcttcc tatacagtag ctataggagg gaaagtcgat gatttaggtg
      541 tcaaaacagc aataggatgc gatttttata tttataacat tcataaatta gcaaatggag
      601 attatgaatt ttgtgtaaaa acgctgaatc cgggtagttt tgacaatttt ttactttggg
      661 atcaaagtgt actggtaatt cagacaactg atcctaattt ttgctatacg accagctcgc
      721 cttatatttc aatccagcaa acgaatatag attcaacata ctgtacgctg tcaacagtat
      781 ttaccttaga tgaagaatgt cctagacttt caaatattac agtagataat tgtacgaaaa
      841 atatcagttt tgaccttaat cttaattata aagcatatgt agattttggg gatggatcag
      901 caatagaggt gtctgactgg ttaggggcat taaatgtatc tcatcaatat agtaataatg
      961 tattgtataa tgtatgtatt acctatgata atggcgaaac gatgcaatac tgctgttatc
     1021 cgattgattt gtctatgtgt accaattctg aatttacacc ggtaatagat acattaagca
     1081 cttcgtgcct taaaccgctt tatcacgaga taccatattg tggcccaagt tcttgtgtga
     1141 gtatttggga gtacgaagat ggtaccgtat attacgggca tacacgccca agccattatt
     1201 ttacgaactt tgtgaatgct gacgatagcg tatggattaa acacacggtg atttgttgta
     1261 atgatacttc tgtaacggtg aaaaagattc cacataaaaa tgctgctttt ataggtactc
     1321 cgggagtaat aaccaatctt tctgatacga tacaatggtc tgatggcccc gacccattga
     1381 ctgctacatt ttataaaatg acagtaaatg actttatact tgcatattcc aataacccaa
     1441 atttaccatt attgatagac ggcacactca atgtggatat caatgcttcc tttggtacag
     1501 gacaatggaa tatgggtaag gggtctgaaa ttgtagttga aggtaaagga ttgagattaa
     1561 gcaatgtaaa tatctggtct gccgtcaacc tgggggtagg ttacgccgca tgttgtgata
     1621 atgaatatac atggttcggc atacgctctg agggaatatc aagaattatt gtatcagaat
     1681 gtactatatc caatgccttg gttgctttgc attatccggg cttaagcgtt actaattcca
     1741 atttcccgaa attgtccgtt ataaacacat cattactcgg caatatgttt gcattacgga
     1801 atgaaaatac ccccttgaat attacaggat tcagcgggaa tttcatacgc ggtcgtcagg
     1861 attgcatctg tacctgtgat gtcgtcaatg ctcttgattt cagagggaaa aacttagctg
     1921 ttgtttttcc gggaggagag accaatgaga tacttcagta cgaacaggga ctgcattcag
     1981 aaaacacctc tgtgacgctg cggaatttct acattcatga cctggacaat tatgccccga
     2041 taaccccttg gcctaatcca agtgatgatt attcgaaaat cggggtgtat tttcaaaata
     2101 caggggactt tgaaggtaat tccgcactca ctcttgagaa ttgtaatttt aatgccatac
     2161 ctaataatga tgaagtaatg cgggtaggag taaaggtgtc tcttgaagca ggcggtcata
     2221 ccttaacagc aattggagac actgttacag aatcaattat tatgaaaaag acaaacaatg
     2281 gttatgccat caatgttgaa aatggcgcgc agatacgtgg tgaaatatca gctaataaaa
     2341 tatatacatc tgatcctgtt aactctgagg gaatatcgct tgaaatgaga tcgcaaaaca
     2401 atcagttaaa tatccaaaac aatattattg atgtcaacgg tggtacctct ctcagcatgg
     2461 gtattaaact tggttcctta aatgaagcag tgcaaaaaat aagggcgata ggaaacaaag
     2521 tttccttagc ttccaataca gctgaaggaa atgccattcg tgttgacaat tgcgaggaag
     2581 tagtcgtaaa ggacaatttt atttattcga attcagaaac accatgcata gaagtgaccc
     2641 aaggcggcaa ctccgacatc aactgcaatt atgtagaggg tggacgaatc ggtcttaaga
     2701 ttgacgctac gtgggataat aatatcataa gcaacagctt cttcaataac ttccagaacg
     2761 ggctgtatgc caatggaaag tgtatagaca ccaaaggatc taaaatagca ctcaactcct
     2821 ttgactataa ctacaatgaa agtatatatt atgatattat gtgtaatacc ggacgacagt
     2881 ggcacgatgg ttataatatg ttttataatc agaacggtga taatgaggta atccacattg
     2941 gagatgaaga gtttgttaaa atgtgtgctt ttcacattaa tcctgcttca gtccttggtt
     3001 cggttaccta tccggatcat gatccctctg gaataatatt gaacaaaacg gtcaaaccat
     3061 acactcacat tgttttcagt tgcagtccgg gtatagatga acctggcaat aagctgtata
     3121 tggataatgt atctatatat gagcgctatg cgggtatctt gtcagatact gcccttgcct
     3181 tgtctgtcac agcggcagag tacgggcatc ttaaacagtc tgtgatggat ttgatagcca
     3241 ggaatcccgg ctggctgcta agcaattcgg tgctgagtga cttttatacg gctgagcaaa
     3301 gtgctttttc aggtaagagc gctgagataa taggattatt cagagcattc cagcagacca
     3361 cgggagtaca gcaggagcat ttacaggtac tgcgtgattc tcacagtgtg gtacagacag
     3421 aaatcctctc cttgatggcc acccttgata cgctgaccaa tccgacagcc atcagctcca
     3481 cgattttgct gatagaggat ctgcagcagc aggcagccca agttatgaat gtacttgatt
     3541 cccttcggtc agtacatgat accctgaaca caacagcgct gacagccctc gccgcagcca
     3601 atggctcact gagcgtttcc gaagattacc aggtatggga gcaggatatg aacgattata
     3661 ctatcaagct tttaaaagga gaagcgcttg attcggccga gcttagccag atacgagcag
     3721 tggcatcaga atgctataga aacggaggta aagccgtact tgatgcacgt attctggcag
     3781 cagcttgtct caaagaatac tattctgtag aggcttgtgc agaaccgcct ggaggtgccg
     3841 gtatgtcatc acaagctcct gagaagttgt atatataccc taatccaggc agagatctgt
     3901 tgaacattag gatcggtgtg gataatgacg caagccaggc agaactaaga gtttatgacc
     3961 tcatgggcag gctgatgcac gtacaagaga cttttgtcaa tacgggtatg gatataacca
     4021 ttgacatatc ccgcctgcct gagggcatgt atgtcgcaga gctgaagaca tcagacagca
     4081 gcttttttga aaaatttatt attcaacgat aagtaatggc taatgccggg cgcatagctt
     4141 gcacccgtca ctatttttta tttataaaaa agtaacggat atgagtaatt tcaacagaaa
     4201 aattaaatat ctaatcatac ttgctttgtc ggtattctgt atcattccga caatttactc
     4261 gcagaatagc tttattggtg ttttttctca tttgacaggt cctgcaaagt ttaacccttt
     4321 tcaagagtat tatcctttta tgttcactca tttagatatt tataaaacaa aagataaggg
     4381 attattatat tgttatcttg gagctcgaga gtgggatgaa aataaaagtc agtttataaa
     4441 tgtaataaag tatgacagtc tttatactcc tctgtggcat aagatttaca tgacaaccga
     4501 agaagcgatg aggccccaaa taatggaaga cagcttgggt agtatatttt tggtatataa
     4561 ccagcatgat tcgatgcttt ataaaatagg tataagtaag ctaaatccgg atggaaatat
     4621 tatctggaca aaaatgctgc ttgatcgttc cagacattcg caattcagat atgccatgtt
     4681 ggcccaaaac ggagatttaa taataagtgg ataccgaata atagaaccag gcaacattca
     4741 agaagcattg gcaatgagag tgaacggtga cggggagata gtctggcaaa agttattcgg
     4801 tggtatcagt agatttttta atgatgttac ggaaacaaaa gacgggtcgc tcctgtttac
     4861 cgcatgggac ggagctgttg taaaattaaa tgccggcggg gcagtattat gggctaattt
     4921 ttatgataag aatgatatac gaacagttct tgaagacaaa gagggtaatt atgttgtagc
     4981 tacagctcct acagggtcgg tttctgtagc gtcaaagtgc cgggttttca gtatcagtcc
     5041 gacaggagag ctgctttgga agaaagacta tttttttgat ttttgggcag gagagggtaa
     5101 cggtctgttt gggggattta tttcccaaat gaaaacatcc ttgcaaatag ttttatggac
     5161 agttaataat tctaagatga tattcctgaa cacggattat ggtgggaata taaactgggc
     5221 atcccgatta cagggtgatg ggtggtttgc gttgaaggat ttaaaccaag ctatggatga
     5281 cgggatattg gtgccgtttt tttgcaatga tggtctgttt gattataagt caaaaggatt
     5341 ctcaggtgtt ataatgacgg atcctgatgg tggtattcct tgtgtcgaga agctgtatga
     5401 cccgcccgtt gatgtagtgg atattgctct tacagcacca accccggcaa caggagttca
     5461 aaatgcttcc tttaagttcg cagatttctc gcttgaggtg gaggatgctc ctatggttac
     5521 attagagatg ctgtgctatg caacagctgt gaggcctgag cctggacgca gggccggctt
     5581 tagcatttac cccaaccctg ctacagaaat gcttaacatc agaactgcca ccgatgtatt
     5641 ggcaagccag gcagaactaa gagtctatga ccttatgggc aggctgatgc atgtacaaga
     5701 gacttttgtc aatacgggta tggatataag cattgacata tcccgcctgc ctgagggcat
     5761 gtatgttgca gagttgaaga catcagacag cagctttttt gaaaaattta ttattcaacg
     5821 ataatccaaa atataggagc gatgaaagat atattgcgca agctctttta tgcctttttt
     5881 atactgacaa ttattgtaag tgcagaaggc aggctttatt cacaaaacag ttttataagg
     5941 atattccagg atacggttta tgattttggt aatgaggtta atgtaagtat ttttgaggca
     6001 aaggataaat caatatacag gacatcctat tattcctcct ataaagagaa tggatcgtct
     6061 gttcctaaaa ttaccttgat caggctggat agtgccggtc aatttatctg gaggaaaagc
     6121 tataagatag cccagaaaga tccgaataac gcaaaagtat tttgccagga taaggcgggt
     6181 aatatctatc ttttttacca ccacaacata acattgccat ttaatcagta tttttcaaag
     6241 ggctatgtct ggaagctcag cccggacggg gagctcatct gggcaaaagg attggaaaat
     6301 acggaaagta cagtagaatt gttggatgga gcgcagtatg ataacgatga tatgctgtta
     6361 tgtggcacat cctttgtgaa atataccaac gggataacgt atgactggaa tccattcgta
     6421 atgcggatca atacggaggg tgagattttg tttcaaaggg aaatttctta tgctagtacc
     6481 ccgccgcatt ctggtaagtt tcaaaaaatt atccgtacac aggatcatgg ctttgtttta
     6541 ttggggctga aaaatagtaa taacagttat tgtgctactg gtgttaccaa gataggtaca
     6601 tatattgcca agctcgacag cacaggtact ttgctgtggg gcaatcaata cccgtgcagg
     6661 gcacctgttg atattttcga aaacaacgac ggtacattta cagtacttac tcaggcttac
     6721 aatggcccgc caagtacttc caaaactttt ggtttgttta acattgactc aaaaggcagt
     6781 gggaaatggg caagggaata cgaatttaca gacaccaaaa tttctgctgt tacagtgagc
     6841 agaatgtttt accgaggcag ttcgatcgat atagtagtga atttgcctaa gagcttaatg
     6901 attctcaact tgagtacaga cggtctgatt aactatgcca atacgataga aaatcaatca
     6961 gacccgaagt tgaatgccta tgactcttat ggcagcgcca tgctgataga agggaaaggt
     7021 tttgttatgc caggatcggt tgaatacaaa aatcccactt tgattaaaac aaatcaaatg
     7081 ggcgaacctc catgcttttt tgatattgtt ccgaacccgg cgattgagaa taacatcgta
     7141 gcgctcaaat tatatgaaaa taacaactcc aaaaggatta cctcttataa gtttacagat
     7201 gatgatgtga taatacaatc aggtctagat tcactgagca acaagctata ttgcctgaca
     7261 acggctgtcc gtgaacaggc actaagtcag gatgaatcgt ttgtcttgta tcctaatccg
     7321 gcttaccaat ctgtgaccat cagctcgcgt gtacaggatt caccggcaat tgacggagtc
     7381 aggctgcatg atatttatgg caatctgttt agatgtacca gcgtcagaat tacccgggga
     7441 gttgagttag atctgacagt tattccacct gggctgtaca ttgttagttt gcagacctct
     7501 gccagaacga tattcaaaaa gctggtggta cagcgttgag cgttgttgat gtattctggt
     7561 tttaatgaaa aactcaagcc aactcagcaa tcactgtttg attgccacgc actatatctt
     7621 caagattcac cttgattttc gcatcgagcg ggagatacag atctactctc gatccgaatt
     7681 tgataaaacc catatcttcg ccctgcttaa cctggttgcc ggtttcgaga tagttgacaa
     7741 ttctgcgtgc aagggcaccg gctatctgtc gcatcaggat tttttctttt ccggtgttgt
     7801 aaactacggt tgtccgttca ttctcggtag atgatttcgg atgccaggct acgagatatt
     7861 ttcccgggtg atattgggca tattctatgg atccgctgac aggattgcgg ttgacgtgta
     7921 cattcagcgg cgacatgaag atagatacca tcagtctttt ttcattgaaa tattcagttt
     7981 ctatcgcttc ttctatcacc actactttcc cgtcagctgg agcatagacc agattgttgt
     8041 ctgccaccgg aattttcctg accggatttc tgaaaaactg taatactata agaaataaaa
     8101 aaccggcaac gatgaccagc agcgcactga tgactaccgg tgtatattcc gcggcagcta
     8161 tcagtaccat acttatgatg gcagctatta cgattgattc gaagccttcg cggtgtattc
     8221 tgaacccaaa cattttatca ttaatttgta caaaggtatc agaattatct tttttaattt
     8281 atagcttttg aataaatatt ttaactcaca tttcagccga cactaataca gaccgtaaat
     8341 aaacaagcga atatacagat aagcaaatgg tatcaagaaa atgaaagcat caaagcggtc
     8401 gagtatgcca ccgtgcccgg gcatgatgtt gcccgaatct ttcacaccat tgtttctctt
     8461 tagcattgat tctgtaaggt caccgagtgt accaaataca acagctataa tacccaatat
     8521 cacccactgg cctgcactga actctccgaa taattccggg aaaaaccagg ctgtaatgat
     8581 agttataaca gctccgccaa tactgccttc ccaggttttt ccgggcgaga tggccggata
     8641 taacttatgc ttgccgaagg ccgatcctgc aaagtaagcc cccgtatcgc ttatccatat
     8701 cagcaccagc aatcccataa tgatgtcagg atggaatccc ttgtcctgta tggcaaggtc
     8761 gcacactaag ctcaccggca cagtaaggta ggcaatgcca agtgctacga atgctacgcg
     8821 tgaaaattgt tgccttgctc tacccagcaa ttctaaaagc attgctatga ataccggtaa
     8881 taaaaacatt gcaatgttga cgaatgttga tgaaatctcc ttgggtaata tcaatttttg
     8941 aaccgctacc cacaggtatg gagaaaaagc aagcaccgtt ccgggtattc ggtactttct
     9001 cagtccttct tcatcacccg tgaagctaag cgtaaacaac tcacgcagcc caataaacat
     9061 gatgacaccg aacaacagca ccaacccgac tttatgcaga taaataccgg cgatcatcac
     9121 aatagcgaaa atggtagccg aagtagcacg cgcacctaaa cttttaaatt tgctcatctg
     9181 tatctgtttt tataccgggc gaatatttgt ataaaatgaa aaataatata ctacacacca
     9241 ccactaccaa caaaggtggg ctatccatct tctcagcatc tatttgttgc gcatcattct
     9301 gtgtcatata cgccaccagt atctgcgaag cgttgaacag aaaatgacaa aatacagcca
     9361 cccatatatt gcctgtctta taaaaaataa accctaacac cacaccaagc aacaatctcg
     9421 gtaagaaacc ttcgaactgg aaatgaagca aactgaaaag gaatgctgtg agaaggatgg
     9481 cctttatatg atgagtatca ccccacatct gtgtaaaaat tcgctgcagg cttcctctga
     9541 agaaccattc ttcaccgatg gcaggtaaca gggcgagcat aacaatgttg aggatgagtg
     9601 aagtagttgt cccttcagat aaaagcattt tcaggaggcc ggatacattt tcttcggtct
     9661 ggcttgccca ttcaggtagc ggtatctttt ggttgaccca ggcagtaaac ataacgagtg
     9721 gtagggaaag gatgaaaagc ccgactgccc atataaattt tttaccctgt tcaccggtag
     9781 attcgattcc tattttgttg aaaatgtatt tgcggtcgct caccatatta tatataaatg
     9841 ccggcagtat aaaggccaat acgctgttga gtacgagcaa cagcctgagt gctgagcgtt
     9901 cacgttcatt acctgtttct atgtttcccg acatcagggc ctggatatcg acggagaata
     9961 cttttgagat ccagaccgat gacaaggata aaaacaatat accgagcagg ccaagaaaag
    10021 caagccgaac cagattcttt acaaaatgcg gtttttcatt ttcttctacc ggaatgttcc
    10081 gttcttcggt tgaatcatct acatttgtca tattaaaaaa ttatgtcatg accagattga
    10141 gcgtaaatat caacaaagtt gcattaatca ggaattccag aggcggaaat ttgccggatc
    10201 ttgtaaaaat agcgattgat tgtgaaaaat ttggggcaga aggcattact gttcatccaa
    10261 gaccagatca aagacatgta cgtttcgacg acattccaaa actaaaatcc gtcgtcacta
    10321 ccgagtttaa tattgaaggc aacccgactc gcgaatttat gaaactcgtc accgacaatc
    10381 ttcctgacca gtgtacactc gtgccggatg cccccggtgt gcttacatcc gactccggct
    10441 gggacactgt tgcgcactac ccatatttaa aagacataac cgaagagctg catgcatata
    10501 atatacgcgt atcgttgttt gtaaatgccg aagagaagta tatagaagct gctgcaaaga
    10561 ccggcacaga cagaatagaa atatataccg gcaaattttc acatgatttt tacacagatc
    10621 ccgggcaggc agtacaagca catatcagat gtgcggcatt ggcagaccac cttggaatgg
    10681 gagtcaatgc cgggcacgat ctcaatcttg ataatctgag ttactataca aagcatgtaa
    10741 agccattgca cgaagtgtcc ataggtcacg ctttgatatc agatgcactg tactacggat
    10801 tgcaaaatac cattcagatg tacaaaaggc agctaataga atttcaataa cgttatatat
    10861 gttgattgat tttaatgagt atatagattt gttaaattaa aaaatgaata atagcatggt
    10921 atttttttca taaaaatgaa aataaactat gttacattaa aaatattaaa t
//
LOCUS       DAOQQY010000063        10723 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2225_length_10723_cov_6.533933, whole genome shotgun sequence.
ACCESSION   DAOQQY010000063 DAOQQY010000000
VERSION     DAOQQY010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10723
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2225_length_10723_cov_6.533933"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLE23_04810"
     CDS             complement(<1..985)
                     /locus_tag="PLE23_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HMX82619.1"
                     /translation="MELKAAKELSDTTQTATNKIIIKGARSNNLKNVDLEIPRNELVV
                     VTGVSGSGKSSITIDTLYAEGQRRYVESLSAYIRQFLGRMKKPEVDYIKGICPAIAIE
                     QKVSSGNNRSTVGSMTEIYDFIRLLYARVGKTISPVSGQVVKKHQVSDVVDFIMSLPE
                     GERVMICMPLTRRFAGKYPKMVLDIIMQKGFGRLVVKNEVIQVEDILSETTFLQSIPA
                     DFHDIQVIIDRIVVERDNIENMQRMADSVNLSFQEAEGECVVIAGGTSTLFNNRFELD
                     GIEFAEPNPQLFNYNNSYGCCRSCEGYGKIIGIDKDKVIPDKHLSVYDGAVA"
     gene            complement(1102..1620)
                     /locus_tag="PLE23_04815"
     CDS             complement(1102..1620)
                     /locus_tag="PLE23_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HMX82620.1"
                     /translation="MLIDIALAVCLVYGFYTGYKNEPLNKIFRVLPFLGGFLFAIYLA
                     PLFYDKLAAMWGSDSIILFALSFILCFFIGWSFSRYLADIFTVGAKKMKISKPEKSLN
                     GVTVSFLLAILFSGLLAFFSKADIISEKTRNNSLSLETLESFPVKLKGSIERFKPGFA
                     KFYEKSNEIIEK"
     gene            1736..2269
                     /locus_tag="PLE23_04820"
     CDS             1736..2269
                     /locus_tag="PLE23_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82621.1"
                     /translation="MINLIINRLIILSIMLLGVVSKQNLFAAEAKAEKEKYTVSSDTV
                     KKPGIFIIGENQAQYELIESKYATSLLTVCKDDINRAFRTWMDVLSEIQKFADKTGFE
                     LKGAKLWLNVFIAPDGTIDYLAYYLQPASRNIPEKEMKAFLLSFIKNNKIPLKCKTGF
                     SHYGQVAFPILPENISN"
     gene            2323..3234
                     /locus_tag="PLE23_04825"
     CDS             2323..3234
                     /locus_tag="PLE23_04825"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMX82622.1"
                     /translation="MLITACQKDVIEADVKSIETHAGMPIEDMLFINDSVGYAVGGKA
                     YNNGIILSTSDGGDTWITDTIIRHGLWAISKNENNEVNATGFSGNLLIKNPGQHWYHY
                     RLPIYNEMTGTLCDREGGVFLCSGMSFQAGKIIHLRTDYSIDTIFETTNEIRSLAYAG
                     EHSLVAVGYGAVFNSDDNGKSWHRSKLDGDYFRKVVFTDPGTGYIAGFSGTLYKTTDG
                     GKNWSQLLSSGHLLKGSDSFLGMAFKDSNEGYICGQYGLLMKTNDGGKNWKHYKRFTE
                     DHLNSITFARNAVLVCSSSGKIYNIHP"
     gene            3324..5225
                     /locus_tag="PLE23_04830"
     CDS             3324..5225
                     /locus_tag="PLE23_04830"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HMX82623.1"
                     /translation="MEGTQYTEDNIRSLDWREHIRLRPGMYIGKLGDGSSPDDGIYVL
                     MKEIIDNSIDEHVMGYGREIKITLKDGIMSVRDFGRGIPLGKVVDCVSQINTGGKYDS
                     KAFKKSVGLNGVGTKAVNALSDYFLVQSFRDGQTKKAVFHYGEITEDDKLQTTEEQNG
                     TLTVFKPDADIFKHYRFRNEFIEQQLWNYAYLNSGLKVNFNGSVYQSKNGLLDLLSRK
                     TEQEEIRYPIVHLKGNDIEIAFTHGSQYGEQYHSFVNGQNTTQGGTHLLAFREAFVKT
                     IRDFYKKDYDAKDIRASIVAAVSVRVEEPVFESQTKTKLGSLNIAPDGPTVRNFIGEF
                     LSKELDNFLHKNPEISQELLKRILQSERERKDIAGIQKLANQRAKKANLHNKKLRDCR
                     YHLNETKVDEDIRSGTTIFITEGDSASGSITKARNVGTQAVFSLRGKPLNCFGMTKKI
                     VYENEELNLLQHALNIEDSIDDLRYNQVVIATDADVDGMHIRLLLLTFFLQFFPDLVR
                     GGHLFILETPLFRVRDKSQTFYCYSEEEKQQATRKLRGKPEITRFKGLGEISPNEFAT
                     FIDENIKLEPVILNDGTNIEQILEYYMGKNSPDRQEFIIDNLRVEKDISENASEELTV
                     SEELAEAAS"
     gene            5334..7745
                     /gene="lon"
                     /locus_tag="PLE23_04835"
     CDS             5334..7745
                     /gene="lon"
                     /locus_tag="PLE23_04835"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMX82624.1"
                     /translation="MDIFDVIMNNQNTEEDAEFMPLVSLDEGEESETTETLPERLPVL
                     ALKNTVLFPGLLVPITISREKSIKALNKAHETNKWIGVLTQKDLKDEDPSFEQLYKLG
                     TVAKIVKILKMPDGTTTAILQGKRRFNVASVTSEDPYLVAEIKYVAYKPISNNMEFKA
                     VIKSIREVAGEIIQLSPNMPPEAKVVLKNITNDTFLLNFMASNLGMKIGVKQDILELN
                     DLTEKAKKVLEQMDAELQLLELKDQIEAKVRGDIEKQQRDYFLSQQLKTIQDELGQNP
                     QEEDIKRLMDKAQKKKWGKEVDRVFKNEINKLKRLNPMVPDYGVSINYLELILELPWD
                     EYTKDNFDLQRVKKVLDQDHYGLEKVKERILEHLAVLKLKGDMKAPILCLVGPPGVGK
                     TSLGKSIAKALKRKFVRMSLGGLHDEAEIRGHRKTYIGAMPGRIIQSLKKVKSSNPVF
                     ILDELDKVGKDFRGDPSSALLEVLDPEQNGTFHDNYLEIEYDLSKVLFIATANTLQGI
                     QPALLDRMEIIEISGYSIQEKVEIARKHLIPEQRKEHGLKAEQVSLSKKLIEQIAQSY
                     TRESGVRSLTRMIGGVMRGVAKKVAMGEDYDKEVKSEDLRQYLGPVIYDRDVYQGVHV
                     PGVTVGLAWTSTGGDILFVESSTHPGKGTLTLTGNLGEVMKESAVTALSFIKAHHQDL
                     GIDTTVFEKMDVHVHVPEGAIPKDGPSAGITMLTALASTYTKRPVKQFLAMTGEITLR
                     GKVLPVGGIKEKILAAKRAGITEIILCEENRRHVEDINQEYLKDMKFNFVSTMNEVLD
                     LALTK"
     gene            7978..10062
                     /locus_tag="PLE23_04840"
     CDS             7978..10062
                     /locus_tag="PLE23_04840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HMX82625.1"
                     /translation="MKTTSNANKATSANALLQEWTGPYNGVPPFDKVQISDFKPAFEA
                     AMHEKNMEINAITRVRSMPDFENTIEALEKAGRTYKRVEAIYEIWKSNMSSPELNTVV
                     SELEPKLAAFRDSVYQNLELFRRIEQVYQSPQMKHLTPEQQRVTWRYYTNFVLAGAKL
                     NESDKAKVAVINQKLASIFSTFSQHLLSDEGDKYLALNSEKELDGMPSDFKEAAAEEA
                     TSKNLKNTWVIANTRSSIEPFLTYATDRNLREKAWNMFVKRGDNNDANDNKALITEIL
                     LLRAERAKLLGYATHAHWRLADKMAKTPENAMKLLESVWTPAVERVKEEVRDMQAIAD
                     KEGAGIKIAPWDYRFYAEKVRKAKYDLDQDEVKQYMQLDKMREAIFFVADKLFDLQFV
                     QVHDVPVFHPDVSVYKVLNKNSGKQIGLWYFDPYARKGKRSGAWMTAYREQEKLSGNV
                     TTIVSNNCNFIKGKPGEPVLISWDDAETLFHEFGHALHGLNSDVTYPLLSGTNTATDY
                     VEFPSQVLERWLSTPEVLNKFALHYKTGKPLPKELLAKIENSSKFNQGFATVEYLASA
                     LVDMKLHLAGAKRIDPAVFEKETLDAYHMPAEIVMRHRMPQFAHIFADDGYSAGYYSY
                     LWSDVLSSDAYEAFTEAGGPYDKTVAKRLHDYVFSVGGTMDEAEAYRKFRGHDPNPDA
                     LMRSRGFVPEKK"
     gene            complement(10129..10563)
                     /locus_tag="PLE23_04845"
     CDS             complement(10129..10563)
                     /locus_tag="PLE23_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HMX82626.1"
                     /translation="MPDSINKERLKDNIVIFDGECVLCSYFFSFLVKYDHRKIYSFAT
                     LQSDTGKKIIAAAWKKEKIPDSVILYQKGKVYAKSAAAIRVVSGLGGIFRLAGLLHIF
                     PKFLSDKIYDFVAKRRYKWWGRQTCLIPDDDIRSRFVDGMRF"
BASE COUNT         3532 a         2141 c         2197 g         2853 t
ORIGIN      
        1 atgccaccgc accgtcgtaa actgacagat gtttgtccgg aattaccttg tctttatcta
       61 tccctataat tttaccataa ccttcacaag accgacaaca gccataagaa ttgttgtagt
      121 taaataattg cggattcggc tcagcaaatt cgataccatc gagttcgaac cggttgttga
      181 acaaagttga tgtaccacca gcaattacta cacattcgcc ttcagcttcc tgaaatgaca
      241 ggttgactga gtcggccatt cgctgcatat tttcaatgtt gtctctttcc accacgatac
      301 ggtcgataat tacctgtata tcatgaaaat cagccggaat agattgaagg aatgtggttt
      361 cggacaaaat atcttctact tgtattactt catttttcac cacaagccta ccgaaacctt
      421 tttgcataat gatatcaagc accattttcg ggtacttccc ggcgaatctc cttgtaagcg
      481 gcatgcaaat catcacacgt tcgccttcag gcaaagacat gataaagtca actacatccg
      541 acacctgatg tttttttaca acctgacctg acaccggtga aatggtttta ccaacacgcg
      601 catacagtaa tcggatgaaa tcataaattt cggtcattga gccaaccgtt gacctattat
      661 tgcctgagct tactttttgc tcaatggcaa tggccggaca tattcctttt atgtagtcca
      721 cttccggttt tttcatccgc ccaaggaatt ggcgtatata agccgacaaa ctttcaacat
      781 atcttcgttg gccctcagca tacagtgtgt caattgtaat ggatgatttg ccagagcccg
      841 aaacaccggt tactactaca agttcatttc ttggtatctc gagatcaaca tttttcaggt
      901 tatttgagcg tgcacctttg ataatgattt tgttagtagc tgtttgcgtg gtgtcagata
      961 attcttttgc tgcttttaat tccataaagt acactgtagt attgctgtga tgaaaaataa
     1021 caaactaaca aaattatagt tttttagctc aattgttggt aagtttgttt caattattat
     1081 agaccaaagt aggttggaaa tttacttttc aatgatttca ttcgattttt cataaaattt
     1141 cgcaaagccc ggtttaaacc tttcaatact gcctttgagt tttacaggaa atgattcgag
     1201 tgtttccagg ctgagtgagt tatttcttgt cttctctgag atgatatctg cctttgaaaa
     1261 aaatgcaagt agtccgctga aaagtattgc taacaagaat gaaacagtta caccattgag
     1321 tgatttttcg ggcttgctga ttttcatttt ttttgcacct actgtgaaaa tatcggccag
     1381 atacctgcta aaagaccagc caataaagaa gcagagaata aaagacagtg caaacaaaat
     1441 aatactatca ctcccccaca tagctgctaa tttgtcatag aataagggtg ccaaataaat
     1501 agcaaacaaa aatcctccta aaaaaggtaa tacacggaag attttattga ggggttcgtt
     1561 tttgtaaccc gtataaaaac cgtaaaccag gcatacagct aacgcaatat cgataagcat
     1621 agacagtttt ggacaatatt gccacaaatt tgaattattt ttatttagtt ttgtgtccgt
     1681 caggtatttt ttattaaaat aaatcgttat atttcacaaa ataaatgccg cacctatgat
     1741 aaatttgatt ataaacagac tcataatatt atccatcatg ttgttgggtg ttgtctcaaa
     1801 acagaatctt tttgccgctg aggcaaaagc tgagaaagaa aaatacaccg tatcatccga
     1861 tactgtcaaa aaaccgggaa tattcatcat tggcgaaaac caggctcaat acgaacttat
     1921 cgaaagtaag tacgcaacat cattgctaac ggtatgcaag gatgacataa accgtgcatt
     1981 caggacttgg atggatgtgt tgagtgaaat acaaaaattt gcagacaaaa ccggatttga
     2041 gcttaaaggt gcaaaactgt ggctgaatgt attcatcgca ccagacggta ccatcgatta
     2101 tctggcgtat tatctgcagc ctgccagccg caacattccg gaaaaagaaa tgaaagcatt
     2161 cctgttatct ttcatcaaaa acaacaaaat accacttaag tgcaagacag gattttcgca
     2221 ttacggtcag gtagcttttc cgattttacc ggaaaatatt tcaaactaaa gtaagcagtt
     2281 ttgagtatat tgaaatattt tggtttcaca ttcataatct gtatgttgat tactgcttgt
     2341 cagaaagatg taattgaagc ggacgtaaaa agtattgaaa cccatgccgg tatgccgatc
     2401 gaagatatgt tatttataaa tgattcggta ggttatgccg taggtggcaa agcttataac
     2461 aacggaataa tactgagcac atcagacggt ggtgatacct ggataaccga taccattatc
     2521 cgtcacggac tttgggcaat atcaaaaaat gaaaacaacg aagtaaacgc aacaggcttt
     2581 tcaggaaatt tactgataaa aaatccgggg cagcattggt atcattacag attaccgatt
     2641 tataatgaaa tgaccggtac actatgtgac agagaaggtg gggtttttct ttgctccggc
     2701 atgtcgtttc aggcaggaaa aattatacac ttacggacag attattcgat tgataccatt
     2761 ttcgaaacca ccaatgaaat aaggagtttg gcttatgccg gtgagcattc tctggtagca
     2821 gtaggttatg gtgctgtctt caactctgac gataacggca aaagctggca ccgtagtaag
     2881 ttggatggcg attatttcag gaaagttgta tttaccgatc ccggtaccgg atacattgcc
     2941 ggtttttcag gaaccttgta taaaacaaca gacggtggta aaaactggag ccaactcctg
     3001 agttcgggac atctgctaaa gggaagcgac agttttctgg gaatggcatt taaagactca
     3061 aacgaaggct atatctgtgg ccagtacgga ctactcatga agacaaacga cggaggcaaa
     3121 aactggaaac actacaaaag atttactgaa gatcatctaa actccataac atttgcccga
     3181 aatgctgtgc tagtatgtag ttcatcgggt aaaatttaca acatacaccc ataaaaaaac
     3241 aaattgttta taaatctgtt tatttcttcg aataatttta taactttaca tccggatttc
     3301 attaaatagc acattcacaa cacatggaag gcactcaata taccgaagat aatattcgtt
     3361 cattagactg gcgcgaacac atacggttga ggccaggcat gtacatcggt aagctgggcg
     3421 atggttcttc acctgatgac ggcatatacg tgctcatgaa agagattatt gacaatagta
     3481 ttgatgagca cgtaatgggt tatggtagag aaataaagat tacgctcaaa gatggaatca
     3541 tgtcggttcg tgattttggt cggggtattc cattgggtaa agtggtggat tgtgtatcac
     3601 agatcaacac aggtggcaag tacgactcca aggcattcaa aaaatcagtt gggcttaacg
     3661 gtgtgggcac caaggcagtc aatgccttgt ccgattattt tttggtgcag tcattcagag
     3721 acggacagac caaaaaggcg gtttttcatt atggtgaaat aacagaagat gacaaacttc
     3781 aaacgactga agagcagaac ggcacactta cagttttcaa accggacgcc gatattttta
     3841 aacattaccg gtttagaaat gaatttatag aacagcagct gtggaactac gcctacctta
     3901 actcaggttt aaaggtaaac ttcaatggtt ctgtttatca gtctaaaaat ggtttgctcg
     3961 atctgttgtc acgcaaaacc gaacaagaag agatacggta tccgattgta cacctcaagg
     4021 gaaatgatat tgaaatagct tttacacatg gctcacaata cggtgagcag tatcattcat
     4081 tcgtcaatgg gcagaatacc actcagggag gcactcattt acttgcattc agagaagcat
     4141 tcgtcaaaac catccgcgat ttttacaaaa aagattatga tgcaaaagac atacgtgcat
     4201 ctatagtggc agcagtaagt gtacgcgtag aagaacccgt atttgagtca cagaccaaaa
     4261 ccaaacttgg ctcgctcaac atagcacccg acggaccaac agtaagaaac ttcattggtg
     4321 aatttctttc aaaagaactc gacaatttcc ttcacaaaaa tccggaaata agtcaagaac
     4381 tactaaagcg gatattacaa tctgaaagag aaagaaagga tatagccggc atacagaaac
     4441 tcgccaacca aagagcaaaa aaagctaatc tgcacaataa aaaactgcgc gattgtagat
     4501 atcatttaaa tgaaacaaaa gtagatgaag atatcaggtc aggcactact attttcataa
     4561 cagaaggaga ctcggcaagc gggtcaatca ccaaggccag gaatgtaggc acacaagcag
     4621 ttttcagtct tcgaggaaag cctttgaatt gttttggcat gaccaaaaag atagtatatg
     4681 aaaatgagga actaaacctt ctgcaacacg ccctgaatat agaagattcg attgacgatc
     4741 tccggtacaa ccaagtcgta atagctaccg atgcagacgt tgatggtatg cacatccggc
     4801 tactcctgct tacatttttc cttcaatttt ttccagattt ggtaagaggt gggcatttgt
     4861 ttattcttga gacacctctg ttcagggtgc gagacaaaag tcagaccttt tattgttatt
     4921 cagaagagga gaagcaacag gctacacgca agctcagagg caaaccggaa attacgcgat
     4981 tcaaagggct tggagaaatt tcgcccaacg aatttgccac attcatcgat gaaaatatca
     5041 aactcgaacc ggtaatctta aatgacggaa ccaacatcga acagatactt gagtattaca
     5101 tgggcaaaaa ttcgcccgat aggcaggaat ttattatcga taacttaagg gtagaaaagg
     5161 atatttcaga gaatgcatct gaagagttaa ctgtttcgga agaactggca gaagcagctt
     5221 cctgacgttt tgtcctgaac ttgccctaaa acaatgcatg gcacattact tgccctgaat
     5281 gaattccaga ctttttttac ttaatgctga cagactgtca aaaaataaca gctatggaca
     5341 tttttgatgt aataatgaat aatcagaaca cagaagaaga cgctgagttt atgccacttg
     5401 tatcacttga tgaaggtgag gaaagtgaaa caacagaaac actgcctgaa cgattgcccg
     5461 tacttgcatt gaagaacacc gtattgtttc cggggctcct tgtacctatt accataagcc
     5521 gcgaaaagtc aattaaggct ctcaacaagg cacacgaaac caacaaatgg ataggcgtat
     5581 tgacacagaa agacctgaaa gacgaagatc cgagttttga acagttgtat aaattgggta
     5641 ctgtagccaa aatagtgaag atactaaaaa tgcccgacgg cactactact gccattcttc
     5701 aaggcaaaag gcgttttaat gtagcaagtg tcacttcaga agatccgtat ctggtagccg
     5761 agatcaaata tgtagcatat aaacccatat ccaataatat ggagtttaag gcggtaatca
     5821 aatctattag agaagttgcc ggagaaatca tacaattgtc gcccaacatg ccacccgaag
     5881 ccaaggttgt actaaaaaac atcaccaacg atactttttt actcaacttc atggcttcca
     5941 atctcggcat gaaaatcggt gtcaaacaag atatccttga actaaatgat ctgacagaaa
     6001 aagcaaagaa agtactcgag caaatggatg ccgaacttca attactcgaa ctcaaagatc
     6061 agatagaagc taaagtacgc ggtgatatcg aaaaacagca aagagattat ttcctttctc
     6121 aacagttgaa aaccattcaa gatgaacttg ggcaaaaccc acaggaagaa gacattaaac
     6181 gcctgatgga caaagcccaa aaaaagaagt ggggcaaaga agtggacaga gttttcaaga
     6241 atgaaataaa taaactaaaa cggctcaatc cgatggtacc tgactatggt gtgagtatca
     6301 attaccttga attgattctt gagttgccat gggacgaata caccaaggac aatttcgacc
     6361 tgcagcgtgt aaaaaaagta ttggaccaag accattacgg attggaaaaa gtgaaggaaa
     6421 gaatccttga acaccttgcc gtattgaagc tcaagggaga tatgaaggcc cctatccttt
     6481 gtttggttgg tcctccgggc gtaggaaaaa cttctcttgg caaatcaata gcgaaggctc
     6541 tcaaaagaaa attcgtacga atgtcactcg gcgggctaca tgatgaggca gaaataagag
     6601 gacatcgcaa aacctatata ggcgctatgc cgggtagaat tattcagtct ttaaaaaagg
     6661 taaaatcatc gaatccggtc ttcatattgg atgaacttga taaggttggg aaagatttta
     6721 gaggcgatcc atcatcagca ttgctcgagg tacttgatcc ggaacagaac ggaacctttc
     6781 atgacaatta tctggagatt gaatatgacc tttctaaagt attattcatt gctacagcaa
     6841 ataccttgca gggcatacaa ccggcactgc tcgacaggat ggaaatcatt gaaatcagcg
     6901 gctattcaat acaggagaaa gtagaaatag ccagaaagca cctgatacct gaacagcgca
     6961 aagaacacgg actcaaagca gaacaagtgt cgttgtcaaa aaaactgata gaacaaatag
     7021 cccaaagtta cactcgtgag tcgggtgtaa ggagccttac cagaatgata ggaggtgtaa
     7081 tgcgtggtgt agcaaagaag gtagcaatgg gagaagatta tgacaaggaa gtaaaaagtg
     7141 aagacctgag acaatacctg ggcccggtaa tttatgatcg ggatgtatat caaggtgtcc
     7201 atgtaccagg tgttacagtc ggtttagcct ggacaagtac cggaggcgat atattatttg
     7261 tcgaatcaag tacacatccg ggaaaaggta ctttaacact aaccgggaac ttaggagaag
     7321 taatgaagga aagtgccgta acagctcttt cattcatcaa ggcccatcat caagatttgg
     7381 gtatcgacac tacagttttt gaaaaaatgg atgtgcatgt acatgtacct gaaggtgcaa
     7441 tccctaaaga cggcccttcg gcaggcatca ctatgctgac agctcttgcc tctacatata
     7501 ccaaaagacc tgtgaagcaa ttccttgcta tgaccggtga gattactctc cgcggaaaag
     7561 tacttcccgt aggtggtatc aaggaaaaaa tacttgccgc aaaaagagcc ggaatcacag
     7621 aaatcatact ttgtgaagaa aaccgccgcc atgttgaaga cataaaccag gaatatctaa
     7681 aggacatgaa gtttaatttc gtttcaacga tgaacgaagt actcgacttg gctttaacaa
     7741 aataagaaaa ccattgtttt tacggataat ctgtaaggaa aacaacttta ttttccttac
     7801 agatttgttt tcgttttgtt aagtatagct tgggttgcag tctattgcgt ttgtaaatgt
     7861 tacctttgtc aaccaaaaca acaaaactgg taaaatatgt ctcgtaaatt aatccaaatc
     7921 tttagtatgt tgattgtact gttgttattc gcacagtgca cgaaaaaact cggtgaaatg
     7981 aaaacaacaa gtaatgcaaa caaggcaaca tctgccaatg ctcttctcca agaatggaca
     8041 ggtccttata acggagttcc accttttgac aaagtgcaaa tctcagattt caaaccggca
     8101 ttcgaagccg ccatgcatga gaagaatatg gaaataaatg ccatcacacg tgtacgttct
     8161 atgcccgact tcgaaaacac tattgaagct cttgaaaaag caggacgtac ctacaagcgc
     8221 gtagaagcaa tatacgaaat ctggaaaagc aacatgagct cccctgaact taatactgtt
     8281 gtttcagaac tcgaacccaa actggccgct tttcgcgatt ctgtttatca gaacctagaa
     8341 ttgttccgca ggattgaaca ggtttatcaa tcaccgcaaa tgaaacatct aactcctgaa
     8401 caacaacgtg ttacatggcg gtattatact aattttgtgc ttgcaggtgc caaactgaat
     8461 gaaagtgata aagcgaaggt agccgtcatc aaccaaaaac tagcatctat attttctaca
     8521 ttcagccagc atttgttatc agatgaaggc gataaatatc tggcactcaa tagtgagaaa
     8581 gagctggacg gaatgccgtc tgattttaag gaagcagctg cagaagaagc aacatcgaaa
     8641 aacctaaaaa atacttgggt aatcgccaat acaaggtctt ctatcgaacc tttccttacc
     8701 tacgctacag acagaaacct acgtgaaaaa gcatggaata tgtttgtaaa gcgtggtgat
     8761 aacaatgatg ccaatgataa taaagcattg attaccgaaa ttttattatt aagagccgaa
     8821 agagcaaaac tgctcggata tgctacgcac gcacactggc gcctggctga taaaatggca
     8881 aaaacacccg aaaatgccat gaaactgctc gaatcggttt ggacaccggc agtagaaagg
     8941 gtaaaggaag aggtgcgcga tatgcaggcc attgccgaca aagaaggtgc tggtattaag
     9001 attgcgccat gggactaccg cttttatgca gaaaaagtcc ggaaagccaa atacgatctc
     9061 gaccaagatg aagtgaagca gtatatgcag cttgacaaaa tgcgtgaagc cattttcttt
     9121 gtggcggaca aactgttcga tcttcaattt gtgcaggtac atgatgtacc cgtattccac
     9181 cctgatgtaa gtgtttataa agtgttgaac aaaaattccg ggaagcaaat tggtctgtgg
     9241 tacttcgatc cttatgccag aaaaggtaag agatccggtg catggatgac agcctaccgc
     9301 gagcaggaga aactctcagg aaatgtaacg accatcgtat caaacaattg caatttcatc
     9361 aaaggaaaac caggcgaacc cgttttgata tcatgggatg atgctgagac tttgttccac
     9421 gaatttggtc acgcacttca cgggctcaat tccgatgtca cttatccgct gttgtcaggc
     9481 accaacacag ctactgatta tgttgagttt ccgtctcaag tactcgaaag atggctgtca
     9541 actcccgaag ttctaaacaa gtttgcactc cattacaaaa caggaaaacc attgccaaaa
     9601 gaattgcttg ccaaaataga aaactcctct aagttcaatc agggatttgc tacggtagaa
     9661 tatcttgcaa gtgcattggt tgatatgaaa cttcaccttg caggtgccaa aagaatcgat
     9721 ccagcagttt tcgaaaaaga aacactagat gcctaccaca tgccggcaga gatagtaatg
     9781 cgtcacagaa tgccacaatt tgcccatatt tttgctgatg acggatattc tgcgggctat
     9841 tacagttacc tgtggtctga tgtgcttagt tctgatgctt atgaggcttt tacggaagct
     9901 ggcggacctt atgacaagac ggtagcaaaa agactgcacg attatgtgtt cagtgtaggt
     9961 ggtacaatgg atgaagcaga agcttaccga aaatttagag gccacgaccc taaccctgac
    10021 gctcttatgc gtagccgtgg ttttgtaccc gaaaagaaat aatcaaagat tattgcaata
    10081 aaatcaagaa agccctgtca tctcgcacag ggctttcttt tttttgggtc aaaaccgcat
    10141 accatctaca aatcggctcc tgatatcatc atcaggtatt aggcatgttt gccgtcccca
    10201 ccatttgtaa cgtcttttgg caacgaaatc atagatctta tcagacaaaa actttggaaa
    10261 aatgtgtaat aacccggcaa gcctgaatat gcctcctaaa ccggaaacaa ccctgatggc
    10321 agcagctgac ttagcataaa cctttccttt ttgatacaaa atgaccgagt ccggtatttt
    10381 ttcttttttc caagcagcag caatgatttt tttaccggta tcagactgta aggtagcaaa
    10441 actgtatatc ttcctatggt catatttcac cagaaaacta aaaaaataac tgcacaacac
    10501 acattcgcca tcgaatataa cgatgttgtc cttaagtcgt tctttattta ttgaatcggg
    10561 catatctggt ctaatattca actgaagagt acttctacca tttcatttat tttaccaaca
    10621 ggacgaagct tcagaacgta tctgtctgga tctattccct tcatattata tttcgaaaca
    10681 aacatttctt caaacccaag cctgtctgcc tcctgaatgc gct
//
LOCUS       DAOQQY010000064        10650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2257_length_10650_cov_7.256725, whole genome shotgun sequence.
ACCESSION   DAOQQY010000064 DAOQQY010000000
VERSION     DAOQQY010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2257_length_10650_cov_7.256725"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..685
                     /locus_tag="PLE23_04850"
     CDS             44..685
                     /locus_tag="PLE23_04850"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HMX82627.1"
                     /translation="MDLVEKKIGEYCERYSTPEPELLKELDRQTHLRTLLPNMLSGHL
                     QGAFLSMVSRMMAPARILEIGTFTGYAALCLAEGLTDDGILHTIEVNEEMEPIIQEFF
                     NKSLLRNKLRLHIGDAKTIIPALNESFDIVFIDAAKLEYAQYYDLVFPFVTKGGYIIA
                     DNVLWSGKVTAEKHDKDTAAIHAFNQKVLNDARTESFILPLRDGLNIVRKISD"
     gene            733..1719
                     /locus_tag="PLE23_04855"
     CDS             733..1719
                     /locus_tag="PLE23_04855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMX82628.1"
                     /translation="MMRKLPEILLPIEKLLHATGVPFVIPLYHTTGLSNHQPYTRHLY
                     QNRTEKEFEHELDWLLKHFEPVELAEIATPMRKNRNKPAFHITVDDGLAVCQEIMAPV
                     LYKKGIPATFFINPAFVGDAEIMHRYKANLLLSHIETDKAALHILSGFLKLPLEFNSL
                     KRFYLDLKYPSRTILDDCAERLGMTWSDLLYGNRLYMTEEQLHNIHEAGFNIGAHSWD
                     HPEYTTLSLQEQLEQTERSVQYVREKFNPTVMSFAFPFTDSGVSADFFKWLDTHVDVS
                     FGCAGLKLDTAASHFQRIPVEKFDYGMRAILLKQTGAFFIKKILGKHIVKHG"
     gene            1712..2836
                     /locus_tag="PLE23_04860"
     CDS             1712..2836
                     /locus_tag="PLE23_04860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX82629.1"
                     /translation="MAESLPYGIVFETYTYRQMCLGSFSMDPSASKIPISRLRFLSQT
                     NNPRALPEDLILVTVKLNGELIAFRTVLPDNIELGVRSVHFAWLSGVWVAPQYRRLGI
                     SRKMFDIIFQAWNGLLLGTEYTPTNGLAITSSGQFGPTISKPGVRIFYKSPIEHIILK
                     KYNKYRPFRYAFKLADTIIDTLVKGRNMMYGNIRLPDEVKYTISEMLSNEAKAFINEY
                     RSYSSTNRRVTELDWIVKYPWLASDPKAKEDSKKYYFSVFAAQFTVCFLEIYQRSDIK
                     AIVMLTLRDGIMKIPYLFINEMDKSDVASCILRYASDHKVKLIVSYHTQLNERLLSLD
                     NKRIIGKKTTRYYLVTKILDEIIPAEALIFADGDGDCVFV"
     gene            2833..3780
                     /locus_tag="PLE23_04865"
     CDS             2833..3780
                     /locus_tag="PLE23_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82630.1"
                     /translation="MSRQIIYLTNAEIDKRKWDNTLSTSPQGRVYAFSWYLDCMSPQW
                     DALILDDYEAIFPLPYTENIPGVKRIFQPRVCQQLGLFMKNGDVTESLMADFFKNIPA
                     HFKSVDLQLHDQTPKPALEGWTISNRTNMILSVSSSYKEIEQAYSEHHRRNIRKSKGT
                     DLKAIDHLTPEKFVNCFIAALNGKIRHYSEAQKNGLISLIKISLNKDAGFIRALGDST
                     GQLLSACFFLQSHGRIYYALPFSTQKGRETSAMYKLLDAIFWEYSGKHIVFDFEGSSI
                     EGVARFNKGFGAISVTFYRIYRENLPWWVKWLLRVRSFF"
     gene            complement(3861..5330)
                     /locus_tag="PLE23_04870"
     CDS             complement(3861..5330)
                     /locus_tag="PLE23_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMX82631.1"
                     /translation="MTNHLTPIMLLLVIVVYFGLLLLVSWFTGKNNSNEVFFKAEKQS
                     PWLLVAIGMIGTSISGVTFVSVPGKVGTEGLNMAFSYMQVVFGYLLGYAVIALVLMPI
                     YYRLNLTSIYGYLESRFGRNSYKTGAGFFLLSRTLGSALRLFLMVIILQKFIFDSFGI
                     PFWLTGFITLAFIFLYTYRGGVKTVIITDTFLALCFISALCLTLYSMMSRLELNVSSL
                     IPALKESGYSRMFFFEGGWNDPNNFFKQFLSGALITIVMTGLDQDMMQKNLTCRTLGD
                     AQKNMFTFSLIIVFVNLLFLALGALLYIYAGKEGIAIPENSDYLYPEIAFHYLTPFLG
                     VVFILGIVAATFSSSDSALTSLTTSFCVDFLDFEKKEQNMAQHRTRMVVHLSFALATL
                     ALLVIFKLTNKDAIINFLFKVAGITYGPLLGLFSFGVLTKYKINDRYVLPVCIIAPLM
                     AWIIDYNSVAWFNGFQFGFLNLALNGLLTFFGLLVIRKK"
     gene            5491..6117
                     /locus_tag="PLE23_04875"
     CDS             5491..6117
                     /locus_tag="PLE23_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82632.1"
                     /translation="MKYFFQFIVVMACLFPFIGCKRTEPPVPFTEKLALCDSLYNFDQ
                     LAEAGKLDKSSFVKYEECLCNTIVPSVQFKTDEGADLSTDKLKGKSYIFYTWNDRQAG
                     FAQDLSYLKKLRDENKGKLEVVALSMFDPAILPPPLPTGLGSMYNITFGIEVLRTGFG
                     LNPNFPMALFIDSKGVVQDIFTGYTYNFRLSEQERRERIDKGVKKILK"
     gene            complement(6197..7303)
                     /locus_tag="PLE23_04880"
     CDS             complement(6197..7303)
                     /locus_tag="PLE23_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82633.1"
                     /translation="MKKLSIAIALVLMGGIATAQEGLPANPKPGTCYVRCISHDEFRE
                     TEQKIMVRPEYNKLEIVPATYKTVEERVIVKEASKKYIYVPATYTTTEVSYVSKEGRT
                     DLSINPATFTDGSETITTHPATSGWEVTTAKNCKSLNPADCQAMCFVERPASTKTVAL
                     KKLGSNASTNNVPVAEQKAAYKKQVIKTPARYDEIDIPAEYATIKRQVVDRPASTKTV
                     TVPAEYVTVKKTELVKAGSIGHYEEIDCKLVNYNALPINWDLASATLTPDAKRIIDEH
                     LLKMLVGKPGVSVEIASHTDSRASSSYNMNLSQRRADSVVNYLVSKGVKRSRLVAKGY
                     GETMLLNKCADGVECSEAQHAVNRRTEFRVINAN"
     gene            7595..8755
                     /locus_tag="PLE23_04885"
     CDS             7595..8755
                     /locus_tag="PLE23_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HMX82634.1"
                     /translation="MNFFLSVNNKIKVSLICFVLLIISGSSTAAIIHISGFVHPTEAP
                     DIKDNQFILVDFWATWCGPCIQAMDHLGRLRQAGGENLVVIGLSNEPYDVVKRFFNKR
                     EPQTYIAVDSDNRTFSGFNITSLPSAALIAPNGEIIWQGKPTHLGELELQKLMSVKYK
                     AMPVSEKISVKQTEQPTLKEDLWTHKEVNRNNIRLSYDGLLTDFGFSISISDTITTIS
                     GKTSDIISALMNVPKARLHLNAEARYGTATFNTTMTNNNRPLLLSLFLEAQDLRYSPV
                     DHQADVSELVVEDSTLLWSNEVYQYSDNGTPVYIIDEGNLQADNYSLPALVDLLTDMI
                     HINFYYPGLCTKTYDWNLDISNVQNLEKQLISEYGIRLKIVNVRLTDFVFSR"
     gene            complement(8758..>10650)
                     /gene="uvrA"
                     /locus_tag="PLE23_04890"
     CDS             complement(8758..>10650)
                     /gene="uvrA"
                     /locus_tag="PLE23_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMX82635.1"
                     /translation="KDKVIPDKHLSVYDGAVACWRGEKSNEYLIALLTTAHLTGFPVH
                     KPYKDLTKAEEKMLWHGTPHFEGIDSFFKMLEEQLYKIQNRVLLARYRGKTNCPECEG
                     TRLRKEASYVKINHTTVGELLEMPIDELAEFFKIIEFSEQERQISSRIMLEINTRLNI
                     MNDVGLGYLMLNRVSNTLSGGETQRINLTRTLGSNLTSSMYILDEPSVGLHPRDTARL
                     VSVLKKLRDLGNTVIVVEHEEEVIKNADYLIDMGPGAGIHGGEVVFAGHYNKIFTEGG
                     NSLTAGYMNGSRVVPLPLHRRKAINRIEISNAGINNLKNISVMIPLNAFTVISGVSGS
                     GKSTLVRDILHPLLKNALESNGNQFAAQLKGDYKRISGIEFVNQSPIGKSSRSNPVTY
                     VKAYDSIRKLMAEQPLSRIRGFEARHFSFNTEGGRCETCQGEGSQLIEMQFLADVHLI
                     CEDCKGRRFKEEVLDVQYNGKNISEILKLSIEEGVEFFKTEKDIVRKLKPLIDVGLGY
                     VSMGQSSSSLSGGEAQRVKLASFLGNDSSGEHIFFIFDEPTTGLHFHDNLKLLDALQA
                     LVEKGHTVLVVEHNIDIIKSADWVIDLGPDAGKHGGEVVFQGTPEGLANEPRSFTGKF
                     LAEKLS"
BASE COUNT         3264 a         2200 c         2132 g         3054 t
ORIGIN      
        1 attcagctac ccttttttga aacgactggc aaaattttaa aatatggatc ttgtagaaaa
       61 aaaaatcgga gaatactgtg aaagatattc aactcccgaa ccagaattgc tcaaggaact
      121 cgaccgacag acacaccttc gaacattgtt acccaacatg ctcagcggac acctacaggg
      181 ggcattcctc agcatggtca gtagaatgat ggctccggct cgaattctcg aaataggcac
      241 tttcaccggg tatgctgcac tctgcctggc ggagggtctt acggatgatg gcattcttca
      301 caccatagaa gtaaatgaag aaatggaacc catcatacaa gagtttttta acaaatctct
      361 cttgagaaat aaactccgcc tacacatagg cgatgccaaa acgattattc ctgccttgaa
      421 cgaaagcttt gatatcgtgt tcatagacgc tgccaagctc gaatatgcac aatattatga
      481 cctcgtattt ccttttgtga cgaaaggagg atacattatt gccgacaatg tattgtggag
      541 cggaaaagta actgccgaaa aacacgacaa agacacagca gccatccatg catttaacca
      601 aaaagtgctg aatgacgcaa gaacagaaag cttcatactg cccctaagag atggattgaa
      661 catcgtacgg aaaatatctg attgattaaa tacgtaataa ttgtcaatac ttgagtaaag
      721 tttgtcatat acatgatgag aaaattaccc gaaatacttt tgcccataga aaaattgctg
      781 catgccaccg gggtgccttt cgtcattcca ctatatcaca ctaccggcct gtcaaaccat
      841 cagccatata cacgacatct ataccagaac cgtacggaaa aagaattcga acatgaactc
      901 gactggcttc tgaaacattt tgaaccggtt gaactggcag aaatagctac accgatgcga
      961 aaaaatcgaa ataaaccggc ttttcacatc accgttgatg acggccttgc ggtctgtcaa
     1021 gaaataatgg ctcctgtatt gtacaaaaaa ggcattccgg ctacattttt tatcaacccg
     1081 gcattcgtcg gggatgctga gattatgcac cggtataagg caaatctact gctgtcgcac
     1141 attgaaacag ataaagccgc cttgcacatt ttgtccggat tcctgaagct acctttagag
     1201 tttaacagtt tgaagcgttt ctacctcgac ctgaaatatc cgtcccgtac cattctggat
     1261 gattgtgctg aaagactcgg gatgacctgg agcgacctgc tgtacggaaa tcggctctat
     1321 atgacagaag agcagctaca caatattcat gaagcgggct tcaatatcgg agcacacagc
     1381 tgggaccacc ccgaatacac aacactaagt ctgcaagagc aactagaaca gaccgaaagg
     1441 agtgtacaat atgtaaggga aaaattcaac cctacggtga tgtcgtttgc ctttccgttt
     1501 acagattcag gcgtttcggc agatttcttc aaatggctcg acacacatgt tgatgtttcg
     1561 tttggctgtg ccggacttaa acttgataca gcagcgtcgc actttcagcg cattccggta
     1621 gaaaaatttg attacggtat gcgtgcaata cttctgaagc aaacaggagc gttttttatt
     1681 aagaaaatac ttggcaaaca catagtaaag catggctgag tcactcccct atggtattgt
     1741 cttcgaaacc tacacatacc ggcaaatgtg tttgggctca tttagtatgg atccttctgc
     1801 ttccaaaata cccatttcga ggcttaggtt tctatcgcag accaataacc caagggcatt
     1861 acctgaagat ctgatacttg taactgttaa acttaacgga gaattaattg catttcgcac
     1921 tgtattgccc gataatattg aactgggtgt aagaagtgtg cactttgcat ggctcagcgg
     1981 ggtgtgggtg gctccgcaat acaggagact gggcatttcg agaaaaatgt ttgatatcat
     2041 ttttcaagca tggaacggat tgctgttagg tactgaatat acacctacca atggtttggc
     2101 tatcacttct tcaggccagt ttgggccgac catttcaaaa ccaggcgttc gcatatttta
     2161 caaatcgcca atcgaacata ttattcttaa aaaatacaac aagtacagac cattccgcta
     2221 tgcttttaaa ttagcagata ccatcattga tactttagta aaagggagga atatgatgta
     2281 tggaaatata aggcttccgg atgaagtgaa atatactatc agcgaaatgc tttcaaatga
     2341 agcaaaagca tttatcaatg aatatcggtc gtattcttct accaaccgcc gggtgacaga
     2401 attggactgg atagttaaat atccttggct ggcaagtgac ccgaaagcaa aagaagattc
     2461 aaaaaaatat tacttttcag tttttgcagc gcaattcaca gtatgttttc ttgaaattta
     2521 tcaaaggtca gacatcaaag ctatagtgat gcttacattg cgcgacggca taatgaagat
     2581 accttatttg tttatcaatg aaatggataa aagcgatgtg gcaagctgca ttctccgata
     2641 cgccagcgat cataaggtga aattgattgt atcttaccat acacagctga atgaaagact
     2701 tttgagcctt gacaataaga gaatcattgg taaaaagacg acaaggtatt atcttgtgac
     2761 caaaatattg gacgagataa tacctgccga agcattgata tttgcggatg gtgatggtga
     2821 ctgtgtattt gtatgagcag acaaataata tatctgacaa atgctgaaat agacaaacga
     2881 aaatgggaca acacactgag tacgtcgcct cagggccggg tgtatgcatt ctcttggtat
     2941 cttgactgca tgtcgccaca atgggacgca cttatacttg acgattatga agccatattc
     3001 ccattgcctt atactgaaaa tattccggga gttaagagga tatttcagcc aagagtgtgc
     3061 caacagttgg gtttatttat gaaaaatggg gatgtaacag aaagccttat ggcagatttt
     3121 tttaaaaata ttccagctca cttcaagtcg gtcgatcttc aattacatga tcaaactccg
     3181 aagcctgcgc tggaaggatg gaccataagt aaccgcacca atatgatttt atcggtgtct
     3241 tcctcttata aggaaataga acaagcttat tcagaacatc accgacgcaa catccgaaaa
     3301 tcgaaaggta cagacttgaa ggcaatagac cacctgacac cggaaaaatt tgtaaactgt
     3361 ttcattgcag cacttaatgg aaaaataagg cattatagtg aggcgcagaa aaatggtctc
     3421 atttcgctta taaagatatc actcaacaaa gatgccggat tcatacgtgc acttggtgac
     3481 agtaccggcc aattgttgag tgcatgtttc tttcttcaat cgcatgggcg tatttattat
     3541 gcattgccat tttcaaccca aaagggaaga gagaccagtg ccatgtacaa gctgctggat
     3601 gccatcttct gggaatattc aggcaagcac attgtgttcg actttgaggg atcttcgatt
     3661 gaaggtgtgg caaggtttaa caaaggcttt ggggctattt cagttacttt ttaccggatt
     3721 tacagagaaa atcttccgtg gtgggtaaaa tggctgttac gcgtacgcag ttttttttaa
     3781 aaaatagaat tttcaaacat taaaacttaa aaaaacgtca cagattcttt acatccaagc
     3841 ttattataac atattacaca ttatttcttc ctgatgacca agaggccgaa aaaagtaagc
     3901 aaaccgttaa gcgcaaggtt aagaaatccg aattgaaagc cattgaacca tgctacactg
     3961 ttatagtcta taatccaagc catcaatggt gctattatac ataccggcag cacatacctg
     4021 tcattgattt tatatttggt aagtacaccg aaactaaaca aaccaagtaa aggaccataa
     4081 gtaatgcctg ctaccttaaa cagaaaattg atgatggcat ctttattcgt tagcttaaaa
     4141 ataactaaca gtgcgagcgt agcaagagca aagctgaggt gtacgaccat gcgggtacgg
     4201 tgctgagcca tgttctgttc ctttttctca aaatcaagaa aatcgacaca aaaagatgta
     4261 gtaagagatg taagtgctga atcgctgcta ctgaatgtag cggccacaat tcccagtata
     4321 aacaccacac caagaaaagg cgtgagataa tgaaatgcta tttccggata cagatagtcg
     4381 gagttttcag gtatagcgat gccctccttg ccggcataaa tataaaggag cgcacctaat
     4441 gcaagaaata ggagattgac aaacacaatg atcaatgaga atgtaaacat gttcttttgt
     4501 gcatccccga gtgttcggca agtcagattc ttttgcatca tatcctggtc gagccctgtc
     4561 attacgatgg taatgagtgc cccactcaga aactgtttga agaaattgtt cgggtcgttc
     4621 cagccacctt cgaagaagaa cattctagag tatccgcttt ctttcaatgc aggtataagt
     4681 gaactcacat ttaactcaag cctactcatc atcgaataaa gtgtcaagca cagggcacta
     4741 ataaagcaaa gggcaagaaa ggtatcggta atgataaccg tcttgacacc accgcggtat
     4801 gtataaagaa aaataaatgc cagcgtaata aaacctgtga gccagaacgg tatgccgaat
     4861 gagtcaaaga taaatttttg caaaataatg accatcaaaa acaacctgag tgcagaaccc
     4921 agcgtacgcg acaatagaaa aaaaccagct cccgtcttgt acgaattccg gccgaacctc
     4981 gactcaagat aaccatatat agatgtaagg ttgagcctgt aatatatagg cataagtacc
     5041 agtgcaataa ctgcatagcc caatagataa ccaaaaacga cctgcatata cgaaaatgcc
     5101 atattgagtc cttctgtacc taccttgccg ggtacagaca caaacgtgac tcccgaaatg
     5161 gaggtgccaa tcataccaat agctaccagg agccagggcg attgtttttc agccttgaag
     5221 aacacctcat tgctgttgtt cttgccggta aaccatgaca caagcagcaa aaggccgaaa
     5281 taaacaacga taaccaataa aagcatgatg ggcgtaagat gattcgtcat tttagcatat
     5341 ttgggtcaaa agtaaacgag ttatcaggtc tgacaaaatt tgaactacac aaatagttgt
     5401 atgtagataa aattttcttt gaatgcttat gaacatccat gaacctaaac caataaaaat
     5461 tcgtagaaaa aatgaaaatt atccggtaaa atgaagtatt tttttcagtt tatagttgtc
     5521 atggcttgtc ttttcccttt cattggctgt aaaagaacgg aacctcctgt tccatttaca
     5581 gaaaaattgg cgttgtgtga cagtctatat aattttgacc agctggccga ggctggcaaa
     5641 cttgacaaat catccttcgt aaagtatgaa gaatgtcttt gtaatacaat tgtgccctca
     5701 gtacaattca aaacagatga aggggctgac ctctcaacag ataagctcaa agggaaaagt
     5761 tacattttct acacttggaa tgataggcaa gctggttttg cacaagatct ttcttatcta
     5821 aaaaagctta gggacgaaaa caaaggaaaa cttgaagtag tagcacttag tatgtttgac
     5881 cctgccatat tgccacctcc cttgcctacc ggtcttggaa gtatgtataa tataacattc
     5941 gggatagaag tactccgaac gggatttggc cttaatccaa attttccaat ggctcttttc
     6001 attgacagta aaggagttgt tcaagacata tttacaggat atacctacaa ttttagactc
     6061 tcggaacagg agagaagaga acgcattgat aagggagtta agaaaatcct gaagtaatca
     6121 agacaaactg tcaattttac acaaaaaaaa agcacttacc gaggcaagtg ctttttttat
     6181 tatagtcatt cagaaattag ttagcattga taactctgaa ctcagttctt ctgtttactg
     6241 catgttgagc ttcagaacat tctacaccat cagcgcattt gttcaaaagc atggtttcgc
     6301 cataaccttt tgctaccagt ctgcttctct tgactccttt tgaaacaaga tagttaacaa
     6361 cagaatcagc tcttctctga gaaaggttca tgttgtaaga gcttgaagca cgtgaatcgg
     6421 tgtgtgacgc tatttcaaca gatacacccg gtttgcccac caacatttta aggaggtgtt
     6481 catcgattat tcttttggca tcaggtgtaa gcgtagcact tgcaagatcc cagttgatcg
     6541 gtagagcatt gtagtttaca agtttacagt caatttcttc gtaatgacca atgcttcctg
     6601 cttttacaag ctcggtcttt ttaacagtta catattcggc aggtactgtc acagttttgg
     6661 tgcttgctgg cctatcaaca acttgtcttt tgatagtagc gtactcggca ggaatgtcga
     6721 tttcatcata tctggcaggt gtcttgataa cctgtttctt ataagcagcc ttttgctctg
     6781 ctacaggcac attatttgta ctggcattgc ttcctaattt cttaagggca acggttttag
     6841 ttgaagcagg tctttctaca aaacacatag cctgacagtc agcagggtta agcgacttac
     6901 agtttttggc agtagtcact tcccaaccac ttgtagcagg gtgcgtagta atggtctcag
     6961 aaccgtcagt aaatgtagcc ggattaatac taaggtcagt tctaccttcc tttgacacat
     7021 aagaaacttc tgtagttgta taagtggcag gcacatagat atactttttg cttgcctctt
     7081 taactattac cctttcttct actgttttgt aggtagccgg cactatttct agcttgttgt
     7141 actccggtcg gaccataatt ttctgctccg tttcacggaa ctcatcatgt gatatacatc
     7201 ttacatagca tgtacctggt tttgggtttg caggtagtcc ttcttgtgct gttgcgatac
     7261 cgcccatcaa tacaagggca atagcaatac ttagtttttt catattattt taatattaga
     7321 aatgaagaaa aatcttaatt caagaacaac tttatgacgt aaaatacgag agaagtaaca
     7381 atatttaaag tttaaaaatt cattaacata attattccat aaaaaactta ctttcctgtt
     7441 taaaaatcct ttaaattcta ctcaattatg tcggtttcta cttgtttcag atgttattaa
     7501 aataatttta actaaatgaa gtatcacagt atagtacttt tgtgcatgga ttaccgttat
     7561 ggcttaatgt atctgaccaa tctgtcaatc aaatatgaat tttttcctat cggtgaataa
     7621 taaaatcaag gtgtcgctta tatgttttgt tctgctgata ataagcggaa gttctacggc
     7681 agctattata catataagtg gttttgtaca cccaactgaa gcaccggaca tcaaagacaa
     7741 tcagtttatt ttggttgatt tttgggctac ttggtgcggt ccctgtattc aggctatgga
     7801 tcatttaggc cggcttagac aagcaggtgg tgaaaatctc gtagtaattg ggctgagtaa
     7861 cgaaccatac gatgttgtaa aaagattttt taacaaaaga gagccccaaa cctacattgc
     7921 tgttgattcg gacaatcgca ctttctcagg ttttaacatc acaagtctgc caagtgctgc
     7981 actcattgct cctaacggtg aaataatatg gcaaggcaaa ccaacccatc tgggtgagct
     8041 ggaattgcaa aagctaatgt ccgtaaaata taaggctatg ccagtctctg aaaaaatatc
     8101 cgtgaagcaa acagaacaac ccaccctgaa ggaagatctg tggacacata aggaagtaaa
     8161 cagaaataat atccggcttt cgtatgatgg gcttctcacc gactttggat tttcaataag
     8221 catttcagac acaattacca ccatttcagg caaaacatct gatattatat ccgcgttgat
     8281 gaatgtgcca aaagccagac tacacctgaa tgcagaagcc cgctatggaa cagccacatt
     8341 caacactaca atgactaaca acaaccggcc actgcttctt tcattgtttt tagaagcgca
     8401 agacctcagg tattcacctg ttgatcatca ggctgatgtt tcagagttgg tagtagaaga
     8461 ttcaacgcta ctatggagta atgaggtgta tcaatatagt gataacggta ctccagttta
     8521 cataattgat gaaggaaact tacaggcaga taactatagc ttgccggcac tcgttgattt
     8581 gctcacggat atgatacaca tcaatttcta ttacccgggc ttgtgcacaa aaacttatga
     8641 ctggaacctt gacataagca atgtccaaaa tctggaaaaa cagttaattt ccgaatatgg
     8701 aatcagactg aaaattgtta acgtccgatt gaccgacttt gtctttagta gataatatca
     8761 agatagtttt tcggcaagaa attttcctgt gaaagatcgc ggttcattcg ccagaccttc
     8821 tggtgtaccc tgaaatacaa cttcgccgcc gtgttttccg gcatccggac caagatctat
     8881 tacccaatcg gccgatttga tgatgtcgat gttgtgttct actaccagta ctgtatgacc
     8941 tttttcaacc aaagcctgca aagcatccag caatttcaga ttatcatgaa aatgaagtcc
     9001 ggttgttggt tcgtcaaata taaagaatat gtgttctcct gatgaatcgt tgcctaaaaa
     9061 tgaagcaagt tttacacgct gggcctcacc accggataac gaagatgaag attgacccat
     9121 acttacatag ccaaggccaa catctatcag tggctttagt tttctgacaa tatctttttc
     9181 ggttttaaaa aattcgacac cttcttcaat gctgagtttg agtatttctg atatattctt
     9241 cccgttatat tgcacatcca gcacttcttc cttaaaccgt cttcctttac agtcttcaca
     9301 tatcagatgc acgtctgcaa gaaactgcat ttcaataagc tgcgaacctt cgccctgaca
     9361 tgtctcacat ctgccacctt cggtattgaa tgaaaaatgc ctggcttcga atccgcgaat
     9421 gcgtgacaat ggttgttcgg ccatcagttt acgaatagaa tcataagcct tgacataagt
     9481 gaccggattg gatcgggacg atttgccaat agggctttgg ttgacgaatt ctatgcctga
     9541 tattcgtttg tagtcgcctt tgagttgagc tgcgaattgg ttgccattac tttccaaagc
     9601 atttttaagc aatgggtgca atatatcgcg caccaaagtt gatttgcccg aacctgaaac
     9661 cccgctaata acagtaaatg cattcaacgg tatcattacc gaaatattct tcaggttatt
     9721 gataccggca ttgctgatct ctatccggtt tatggctttt cgtctgtgta acggcaatgg
     9781 tactacgcgg ctaccgttca tatatccggc tgtaagactg ttgccacctt cggtgaatat
     9841 tttattgtaa tgccctgcaa ataccacttc gccaccatga ataccggctc cgggtcccat
     9901 atctatcaga taatcggcat ttttaatgac ttcctcttcg tgttctacaa cgatgactgt
     9961 attgccaagg tcgcgtagct ttttcaatac agataccaag cgtgctgtat cacgaggatg
    10021 caaacctaca cttggctcat caagtatata cattgaactt gtaaggttgc tgccgagtgt
    10081 acgggtcagg ttgatacgct gtgtttcgcc accactcaat gtattggata cacggttgag
    10141 catcagataa ccaaggccta catcgttcat tatgttgagc cttgtattta tttcaagcat
    10201 tattctagat gaaatctgcc tctcctgttc agaaaattcg attattttaa aaaattctgc
    10261 cagttcgtca atcggcattt cgagcaattc tcctacggtt gtatggttga tttttacata
    10321 agatgcctcc ttacggagtc tggtgccttc acattccggg cagttggtct taccacggta
    10381 tctggccaac aatacacgat tttgaatttt atatagctgt tcttcgagca ttttgaaaaa
    10441 cgaatctatg ccttcgaagt gtggagtacc gtgccacagc atcttctctt cagctttggt
    10501 gaggtcttta taaggcttat gtaccggaaa acccgtaagg tgtgcagtag tcaggagtgc
    10561 aataagatat tcattcgatt tttcacctcg ccaacatgcc accgcaccgt cgtaaactga
    10621 cagatgtttg tccggaatta ccttgtcttt
//
LOCUS       DAOQQY010000065        10513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2295_length_10513_cov_6.187321, whole genome shotgun sequence.
ACCESSION   DAOQQY010000065 DAOQQY010000000
VERSION     DAOQQY010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2295_length_10513_cov_6.187321"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLE23_04895"
     CDS             complement(<1..681)
                     /locus_tag="PLE23_04895"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HMX82636.1"
                     /translation="MPGIKYLRIRFDQNIFPYDIPKFRAAVIEKTEREAALFHNHIGD
                     TQFDYRYPLIQYKVTNKKASIICLEEGTDQIHQLFLKNDLNLRVGNTHAEYRVEDISL
                     NKHLVQLWNTTFEYSLLNWLPLNQQNHKKYLSLDDNLPEQVKILERSLLGHILAFASG
                     IGWQVEGRITADITDIKEMKMLEYKRQKMLAITLTFKSNVSFPDYIGLGKGGSVGFGI
                     VKRISKENV"
     gene            complement(934..1308)
                     /locus_tag="PLE23_04900"
     CDS             complement(934..1308)
                     /locus_tag="PLE23_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82637.1"
                     /translation="MSEPKLPFTYKKNLHPNSIFNSEHKYALTVNNFEPGTYQGVLTG
                     NVVYKPSTKVPGGKELYLQVDFPETINGMLVKSSPDIEEFVNEHGAAQLKGRLFSFAI
                     KGFQRSDGVFIRYFDTDNQYVE"
     gene            complement(1362..2960)
                     /locus_tag="PLE23_04905"
     CDS             complement(1362..2960)
                     /locus_tag="PLE23_04905"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX82638.1"
                     /translation="MPSVFTFFNGMTLTDSQSRAIEAILAFLADEKARVFILKGYAGT
                     GKTTIVKGLTDYLSSEKKTFHCLAPTGRAARVMSAKSGRVARTLHSEIFTKAKTTVNP
                     DTQSFHTKFVLKDQTPMDGTIYMLDEASMLSDNESDSGENLNFGSGRNMADFFTYLKM
                     EENSKTKVIFIGDEAQLPPVKSTFSPALDAQYIQTVFGYECREATLSDIVRYDNEILD
                     AATLVRESITSKVLNSHIPLSAGDVEVVRGYQSLIKAYMNKVHDVEDTRSHMILAFSN
                     SAVDQYNGIIREKLFPESKNELCAGDRIMIAANNYQGDDILMNGEFADILSVGRLEIV
                     IERIKANYHIEERDSVKQVGQNEYEVELHFRDALIKVSGGAEQEVKLIENLFYSGAAG
                     MTREVRQALFTNLWRRISEENPHKEKDEIKRRVETRISTDPYINAVYAKFGYAVTGHK
                     AQGGEWNSVFVNPWGAKPDTLEHYRWIYTAYTRAARHLTIEWTQPVENKKVEEPPRIY
                     PVRKVVQTTITTPDGKILSKKPKL"
     gene            complement(3259..3624)
                     /locus_tag="PLE23_04910"
     CDS             complement(3259..3624)
                     /locus_tag="PLE23_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMX82639.1"
                     /translation="MEQTSRRKLKDYNPGNCPVVYSLNKIGNRWKLLIIHYIRQGHNR
                     YSTLQRVIPDISRQTLTNQLRELEADGILDRVIYQQIPPRVEYNLTEVGHSLQPILRE
                     MSKWGLQYVTPAGYEPGKC"
     gene            3722..4657
                     /locus_tag="PLE23_04915"
     CDS             3722..4657
                     /locus_tag="PLE23_04915"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HMX82640.1"
                     /translation="MQAVQINKYGDNSVLEQVDIPIPKILPDQVLVKVKYASVNPVDY
                     KIRSGFMAGVLPKSFPFTLGWEASGIITDTAHDVLNYKTGDEIILMSNFMQGGTYAEY
                     VAVNAGEIMSKPTSLDFADAAVIPFSLGTAYTALLMDTQIMPGQKLLILGAGGAVGRM
                     AIQIAKNLGLEVTGTAKGNDIQELLSLGIDHVVDYTKADYFDALNDFDVVLDLVGGET
                     QAKSFKTLRKGGTIVSTTQPPDSGELQHYGLNGKMTRTKCDPEKFAVPLQWLEEGKIK
                     VKEPQIMNFGSAKEALALVENREAKSKIVLSLKYL"
     gene            complement(4717..6429)
                     /locus_tag="PLE23_04920"
     CDS             complement(4717..6429)
                     /locus_tag="PLE23_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HMX82641.1"
                     /translation="MQIQAYQFVKYDEHTTIEQIERVHDTGAVVCFDFEDGMLNPFTP
                     EESATIKAKARSIFIRLYAAILASGKYINIGVRINHHHTSHFELDLLALAGKSFDAVF
                     LPKTECADDMNHFLKSFKAYKIGFKYLIPIVESKQGMENLQTILQASPMVSKVAFGHC
                     DYNLDIGAYPFFHQDSLEYWKWINALKAGMGKAEVQIINSPFLDTGDEAFFCKILSYI
                     ANCGQQFCGQVTLTTRQSVLCREITISTENFIKSLHNRHKIAVDKSVAWQLAEEFESS
                     NKQKGLTRNKQKFISLQEYLASKKFLSDTRPVFEFCFVGGCFPVQNDIVYENLFHQLL
                     KRNIEQTQSIKFNVNIIRYESFGTVTDKLKAFSSQKKPDLVVFHVRPEPYLRLVKLLY
                     KYVDHQGVKHWSFNLPFLNILKSEKYDMLDAGRIYQVNNIQRKSILHQLLISFNYLAG
                     CLIGNKHFALKKYLQTVHSILDYCNENGINILILGPNRRNNNYLEPALCRALDQYFAN
                     NTAEQTYVRGYDTEIKIQMNQKYGIYATLDYHKFVATKLYKAIFAQNLLPTLLCKSKQ
                     EGNS"
     gene            6696..7193
                     /locus_tag="PLE23_04925"
     CDS             6696..7193
                     /locus_tag="PLE23_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HMX82642.1"
                     /translation="MPEERPRLKIIHTTSDLLMEITGWILLVMVWFIVLKAYNSLPDI
                     IPTHYSANGNADGHGPKVSILLLPAISTIAYIGMTILNKYPHIFNYPAVITRENALKQ
                     YTMATWMIRVLKLAIVVLFGVIALVTLRNVKTGQEDLGAAFLPASMLLIFVPVAYFTI
                     KSLKK"
     gene            7316..7891
                     /locus_tag="PLE23_04930"
     CDS             7316..7891
                     /locus_tag="PLE23_04930"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HMX82643.1"
                     /translation="MKKGRLEAFSDGVFAIIITIMVLELKVPEGEHFKNLTPLVPKLL
                     SYLLSFIYVGIYWNNHHHLFQAAERVNGKILWANLSLLFSLSLIPFTTAWVGENHFSE
                     NPAALYGINLLLCAITFIILEKSVIRHEGKESLLGKVLEVKIKENASVFLYTSGVFLS
                     FYMPALSMICYAIVAAIWLIPDKRIEKMLNN"
     gene            8057..8854
                     /gene="proC"
                     /locus_tag="PLE23_04935"
     CDS             8057..8854
                     /gene="proC"
                     /locus_tag="PLE23_04935"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HMX82644.1"
                     /translation="MSKNIAIIGGGNLGASIADGLLRSGFCMPQQLSITRRTVAPLKR
                     FAEAGCFVSYDNAAAIKRSEVIILAVKPYNYTDILKEINPALNAQQHLLVSVITSISI
                     GQLVQSIEKDVAVVRAMPNTAIAIQQSMTCLTHTGVSEVKLKYVEELFNHLGRTVIID
                     ENLMDAATVLGACGTAYAMRFIRANIQGGIEIGFDAATANLIAAQTIKGAAHLLLEKG
                     THPEQEIDKVTTPKGCTIAGLNEMEHQGFSSSLIRGLVTSYRKINEK"
     gene            complement(9013..9507)
                     /locus_tag="PLE23_04940"
     CDS             complement(9013..9507)
                     /locus_tag="PLE23_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HMX82645.1"
                     /translation="MKQLDVKGQTIIKTNYEIVKVEDGDGIIAKNIISNKEEEIRFYG
                     IDAPELTPCKKLKQDERETHLSGRFLMELAHQSYEFLRTKAKIGKRITLIQEVGNEQD
                     PYGRTLAYVVLPNGNTLNEIMVKNGFAKPYDKLYCSELPKYQELNLRAKTRKKGLYLL
                     VDRF"
     gene            9589..10392
                     /locus_tag="PLE23_04945"
     CDS             9589..10392
                     /locus_tag="PLE23_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82646.1"
                     /translation="MARQKGHIKYVGTLGEVRHFKIKGKDGYFAGLKGGPSAEQIATD
                     PAFVRTRENMNEFGGSAIAGKSLRTSIASLIRNNGDSQVTGRITAIMKRINLEDGSEA
                     RGQRAILVTVAPQYLEGFEFNKFTSFNGAFNAPYTITPTATRDSSTLDIPSFNPLDRM
                     YIPAGATHFKIINAIAVLSDFEYNSTTGVYEPKDGTTNGLTDVQDSGYLPVNAATGVF
                     SIVSTLPGSPTISADVSVVNVLAVEFYQEVNSNYYLFSQGNAMKVAKIF"
BASE COUNT         3062 a         2082 c         2024 g         3345 t
ORIGIN      
        1 tacattttct ttacttatcc tcttcacaat accaaagcca acactaccgc cctttccaag
       61 accaatataa tccgggaagg agacatttga cttgaaagtc aatgttatag ccagcatctt
      121 ttgtcgcttg tattccaaca ttttcatctc ctttatatct gtaatatcag ctgtgatgcg
      181 gccttctacc tgccagccta taccactggc aaaggccagg atgtgcccca gcaagctacg
      241 ttcaagtatc tttacctgct cgggcagatt gtcatcgagc gataggtact ttttatggtt
      301 ctgctgattg agcggaagcc agtttagcaa tgaatattca aaagtggtat tccagagctg
      361 gaccaggtgc ttattgaggc tgatgtcttc tacacggtat tcggcatggg tatttccgac
      421 tctaagattg aggtcattct tgagaaaaag ttgatgaatc tggtctgtac cttcctcgag
      481 gcagatgatg ctggcttttt tgttggttac cttgtattgt atcaatggat atcggtagtc
      541 aaattgcgtg tcgcctatat gattgtgaaa caacgctgct tcgcgttctg tcttttcaat
      601 gaccgcagct ctgaatttcg ggatatcata aggaaagata ttctgatcaa aacgaatacg
      661 taaatatttg ataccgggca tcttatggct ttttcgttct ttggttttgc atattaaaga
      721 ctgttttcac gggtaataat ttgctcaata atgtaaaatt aaggactctg tcattcagaa
      781 tcaagtattc acaccactta atcagccaaa acatttcagc caaaataaaa ttttatacat
      841 cactatattt tatcaacaat attttgcaat cattcaccgc actcatgcca tacaagcttg
      901 tgttttcgtt acatccagaa gttggataaa tatttactca acatactgat tgtcggtatc
      961 aaaatatctg ataaacacac cgtcactgcg ctgaaatcct tttatagcaa aactgaataa
     1021 cctacccttg agctgggcag caccatgttc atttacaaat tcttcaatat cgggtgatga
     1081 tttaacaagc ataccgttga tggtctccgg aaaatctacc tgaagataca attctttgcc
     1141 tccgggtacc ttggttgatg gtttgtacac aacattaccc gtgaggacgc cttgatatgt
     1201 accgggctca aaattattca ccgttagagc atatttgtgt tctgaattga agattgaatt
     1261 ggggtgtaaa tttttcttat aagtaaaggg cagtttgggt tcagacatag gattatattt
     1321 tgattgcatt aacaaatatt ttatgattta tagcagttat ttcagagctt tggtttcttg
     1381 ctcaggattt ttccgtcagg tgtggtgatg gtagtctgta ctaccttgcg cacaggataa
     1441 atccttggcg gttcttctac ttttttattc tccaccggtt gtgtccattc aatggtaaga
     1501 tgtctggctg cgcgtgtata ggccgtatag atccaacggt aatgttcgag tgtatcgggc
     1561 ttggcacccc aaggattcac aaaaaccgaa ttccactcgc cgccttgtgc cttgtgccct
     1621 gtgacggcat agccaaattt agcataaaca gcattgatgt atggatcggt tgatattctg
     1681 gtttctactc tgcgctttat ttcatccttt tccttatgcg gattttcttc acttatccta
     1741 cgccagagat tggtgaacaa ggcttgtcgt acttcacgag tcataccggc ggcaccactg
     1801 taaaagaggt tctctatcag ttttacttcc tgctcggcac cacctgatac ttttattaaa
     1861 gcatctctga agtgcagttc tacctcgtac tcgttttgcc ctacctgctt aaccgaatcc
     1921 ctttcctcaa tatggtaatt tgccttgatg cgttcaatca ctatttccag ccggcccacg
     1981 ctaagtatat cagcaaattc gccattcatg agtatgtcat cgccctgata gttattggca
     2041 gctatcatta tccggtcacc tgcacacagt tcatttttac tttcaggaaa aagcttttcc
     2101 cttattatgc cattatactg atcaacagca ctattgctga aagccagaat catgtgactg
     2161 cgggtatcct ccacatcgtg aactttattc atatacgctt tgataagtga ttgatagcct
     2221 cttaccactt caacgtcacc ggccgacaac ggaatgtggc tgttcagcac cttgctggta
     2281 atagattcgc gaaccagcgt ggcagcatcg agaatttcgt tgtcgtagcg tacaatatca
     2341 gataatgtag cttctctgca ttcataaccg aatactgttt gtatatattg tgcatcaagt
     2401 gcaggcgaga aggtactttt taccggaggt aattgtgctt catcacctat aaatatgact
     2461 ttcgttttgc tattttcttc cattttgagg tatgtaaaaa aatcagccat atttcttcct
     2521 gagccgaaat tcaaattctc accagagtct gactcattat ctgacaacat cgaagcttca
     2581 tccagcatat agatggtgcc atccattgga gtttgatctt tgagcacgaa tttagtatga
     2641 aaactttggg tatccggatt tactgtcgtc tttgctttgg taaatatctc agaatgtaat
     2701 gttcttgcca cgcgtcctga tttggcagac atgactcttg ctgcccgacc tgttggtgca
     2761 aggcaatgaa aagttttttt ctctgatgat aaataatctg taagcccttt aactatggtt
     2821 gttttacctg taccggcata ccctttgagt ataaataccc tggctttttc gtcagcaagg
     2881 aatgcaagga tagcttcgat ggccctactc tggctatcgg tgagtgtcat accattaaaa
     2941 aaagtaaaaa cggatggcat tatatgttca ggtttttttg gagttttatg aaaaataata
     3001 ttcaggcttg gtactgctta atgcaatatt taaattaccg gataaaatta cttgaaaaac
     3061 aattaaaatg caagtaatta tcctttacac atgtaatatc attacattaa atgaatcatt
     3121 taacaggtac ttcggtggta tgtgaccata aagcttatga tacaaataga ttgtacccgg
     3181 acaaacagtt tactcgcaat gttgtgagcc gtaatttttc ctcacgtgac aaagtatcgg
     3241 atattctacc ctgcgagctt aacatttacc cggctcgtat cctgccggtg taacatactg
     3301 cagaccccat ttgctcattt ccctaagtat aggttgcagg ctgtgcccca cttcagtaag
     3361 attgtattct acccttggag gaatttgttg ataaatgacc ctgtctaaaa taccatctgc
     3421 ttcaagctcc cgaagctgat tggtcaaagt ctgtcggcta atatccggaa tgactctctg
     3481 caaggtactg tatcggttat gtccttgtct gatgtagtgt ataatgagta atttccacct
     3541 gttaccaatc ttgttaaggc tatacactac cggacaatta ccagggttgt agtccttcag
     3601 ctttctgcgt gatgtttgtt ccattagtct aaaatattag cgttagacaa ttttttttga
     3661 ttaatctttt tacaagacaa atgtacataa ttttgcccca caaattttaa tttttaccat
     3721 catgcaggca gttcaaatca acaaatacgg cgataattca gtattggagc aggtagatat
     3781 tcccattcca aaaatattac cggaccaggt gctggtaaag gtaaagtacg cttctgttaa
     3841 tcctgtcgat tacaagattc ggtcaggctt tatggcaggt gttttgccaa aatccttccc
     3901 ttttacactt ggttgggagg catcaggcat tataacagac actgcgcatg atgtgctcaa
     3961 ctataagacg ggtgatgaga tcattcttat gtctaatttc atgcaaggag gaacgtatgc
     4021 agagtatgtg gctgtcaatg ccggcgaaat catgtcaaaa cccacatctc ttgactttgc
     4081 tgatgcggct gtcattcctt tttctcttgg cacagcttat actgctttgt tgatggatac
     4141 tcagattatg cccggtcaaa aactcctgat actcggtgcg ggtggtgccg taggtcgcat
     4201 ggccatacag attgcaaaaa atctcggact ggaagtaacc gggacagcta aaggcaatga
     4261 tatacaagaa ctattgtcac tgggtattga ccatgtagtt gattatacaa aagcagatta
     4321 ttttgatgca ttgaatgatt ttgatgtagt acttgacctg gtaggtggcg aaacacaagc
     4381 aaaatctttc aagacattaa gaaaaggcgg caccatcgta tcgactaccc aaccaccgga
     4441 tagcggagaa ttacaacact atggattgaa tggtaaaatg acccgtacaa aatgtgatcc
     4501 agaaaaattt gctgtgccac tccaatggct cgaagaaggc aagataaaag taaaagaacc
     4561 tcaaatcatg aactttggct ctgccaaaga agctttggca ctggtagaaa atagagaagc
     4621 gaaatcaaaa attgtattaa gccttaagta cctgtaatct caaagctaat actacattga
     4681 ttgtgcttgc acggttcggg ttttagtatt tatgagctaa gagttgcctt cctgtttact
     4741 tttacacagt aatgtaggaa gaagattctg ggcgaatatc gctttgtaca gcttcgttgc
     4801 aacaaacttg tgataatcta gtgtagcgta aattccatac ttctggttca tttgtatttt
     4861 tatttctgta tcataacctc gaacatacgt ttgttctgct gtattattgg caaaatattg
     4921 atctaacgcc cggcataagg caggctccag atagttatta tttcttcggt ttggtcctaa
     4981 aatgagaata ttaataccat tttcattaca ataatcaaga atagaatgca cagtttgcag
     5041 atattttttt agcgcaaaat gcttattccc tatcaagcaa ccggccaaat aattaaagct
     5101 aatgagtaat tgatggagga tggatttcct ttgtatattg ttgacctgat atattcttcc
     5161 ggcatcgagc atgtcgtact tttctgactt cagaatattc agaaaaggca gattgaacga
     5221 ccagtgtttc acgccttggt ggtcaacata tttatacagc aactttacca gtcttaagta
     5281 tggttcaggt cgtacatgga acaccaccaa atcgggtttt ttttgagaag aaaatgcttt
     5341 taacttgtct gttactgtac caaagctttc atacctgatg atattgacat tgaatttaat
     5401 gctctgagtt tgttcgatat ttctcttcaa caattgatga aaaagatttt cataaacaat
     5461 atcattctgt accggaaaac acccacctac aaaacaaaat tcgaatacag gccttgtgtc
     5521 actcaaaaac tttttggagg cgaggtattc ctggagtgag atgaattttt gcttgttcct
     5581 tgtaagacct ttttgcttat tggacgactc aaattcttca gcaagctgcc atgccacgga
     5641 tttatccaca gctattttgt gccggttgtg taggcttttg atgaaatttt ctgttgaaat
     5701 cgttatttcc ctgcataaga ccgactgtct ggttgtaagg gtaacctgac cgcaaaattg
     5761 ttgtccgcaa ttggcaatgt aactcaatat tttacaaaag aatgcttcat caccggtatc
     5821 cagaaagggt gaattgatga tttgtacctc tgcttttccc atacctgcct tcaacgcatt
     5881 tatccatttc caatattcga gactgtcttg gtgaaaaaac ggatatgcac caatatcaag
     5941 attataatcg caatgaccaa atgccacctt actaaccatt ggcgatgctt gtaggatagt
     6001 ctgaagattc tccattcctt gttttgactc cactatcggt atcaagtatt taaaccctat
     6061 tttgtaagcc ttgaaagatt tcaggaaatg gttcatatca tctgcacatt ctgttttggg
     6121 taagaaaaca gcgtcaaaac ttttaccggc tagtgctaac aaatcaagct caaaatgtga
     6181 tgtatgatga tgatttattc ttacaccaat gttgatgtat ttgccacttg ccagtatggc
     6241 agcatacaaa cgaatgaaaa tggatcttgc cttggcttta atggtagcag attcttcagg
     6301 agtaaatgga ttgagcatac catcttcaaa atcaaaacaa accacagccc ctgtatcgtg
     6361 cactctttct atctgttcga ttgtagtgtg ttcatcgtat tttacaaact gatatgcctg
     6421 aatctgcatg gtcttttggt taattaattt ttaggctatg ttaaaagtat attcccggtt
     6481 catctttagc tttacagtca gatagaggtc atgtgccgac gaattattag cattcaaatg
     6541 taatacgtcg tcccgactac acaaaattta tttatacatt gcgtatgtta ctatccttaa
     6601 ttttgatagc ttaataagaa aaactgcaag ttgattaata tctttgttat ggctctttga
     6661 ttatttattt ataaaaatcg aatattacca agataatgcc ggaagaaagg cccagactga
     6721 aaatcattca taccacatcc gatctgttga tggaaatcac cggctggatt cttctggtga
     6781 tggtgtggtt catcgttctg aaagcttata acagcctgcc ggatattatc ccgacacatt
     6841 atagtgcaaa tggcaatgca gatggccacg gaccaaaggt aagtattctt ttactcccgg
     6901 ctatcagtac cattgcatat atcggtatga ccatactgaa taaatatccg catatattca
     6961 attatcctgc tgtcatcacc agggagaatg ctttgaaaca atacaccatg gctacatgga
     7021 tgatcagagt tttaaaatta gcgattgtag tattgtttgg agtaattgct ttggtcacac
     7081 tccgaaatgt aaaaaccgga caagaagatt tgggtgctgc atttcttccg gctagtatgt
     7141 tgttgatatt tgtacctgta gcatatttta ccataaaatc attaaaaaaa tgacgattta
     7201 taaaaaaatg aaacaaactc aatcatgaca ttaacacaat caaaaaattt tgaataacaa
     7261 aaaggataat ccaacaaatg aaaagattat ctttacttca taaccaacaa tataaatgaa
     7321 aaaaggacgg ctcgaggctt tcagcgacgg tgtatttgcc atcatcatca ccataatggt
     7381 tttagaattg aaagtaccgg aaggtgaaca ctttaaaaac ctgacaccct tggtgcccaa
     7441 actactgtcc tatttactga gctttatata tgtcggcatt tattggaaca accaccacca
     7501 tctgtttcag gccgccgaaa gagtaaacgg taaaattctg tgggccaatc tttctttatt
     7561 attttcactt tctttgatac ctttcactac ggcttgggta ggagaaaatc atttcagcga
     7621 aaaccccgcc gcattatatg gtatcaatct tctattgtgt gccatcacct tcattatcct
     7681 cgaaaaatct gttatccgcc atgaaggcaa ggaatcgctt cttggcaaag tacttgaagt
     7741 aaaaatcaaa gagaacgctt cagttttcct ttatacttca ggggtttttc tgtcatttta
     7801 tatgccggca ttgagtatga tatgctatgc aattgtagca gcaatatggc tcatacccga
     7861 caaacgaatt gaaaaaatgc taaacaactg atactgcttg gaaaagactc attattgtta
     7921 aaggcttgtt tctcttagaa aatatcctga gttccttctg acttgaattc tatttgtcat
     7981 attccataag agttgttgat gaaatgcaac tacatcattc atttggagta caataagcta
     8041 ttgaaacaat tattgaatgt caaaaaacat agcaatcata ggtggaggaa acctgggagc
     8101 ctctatagct gacggcctct tgaggagtgg cttttgtatg ccacaacaac ttagtatcac
     8161 tcgtcgtaca gtggccccgt tgaagagatt cgctgaggca ggatgttttg taagctacga
     8221 caatgctgca gctatcaagc gaagtgaggt aattattctg gccgtaaagc cttacaatta
     8281 tactgatata ctcaaagaaa taaatccggc actcaatgca caacagcatt tgttggtatc
     8341 ggtaataaca agcataagca tcgggcagct tgtacagtca atagagaagg acgtagcagt
     8401 agtacgggct atgcccaaca cggctatagc catccagcaa agtatgacct gccttacaca
     8461 tacgggcgta agcgaagtta agcttaagta tgttgaggaa ttatttaatc acttaggtcg
     8521 taccgtcata atcgacgaaa acctcatgga tgctgctact gtacttggtg cttgtggtac
     8581 ggcttatgcc atgcgtttca tcagagccaa catccaggga ggtatagaga taggttttga
     8641 tgcggcgact gccaatctta ttgcagcaca gactataaaa ggagcagcac acctgctact
     8701 cgagaaaggc acacatcccg agcaggaaat cgacaaggtg acaaccccca aaggatgtac
     8761 cattgccggc ctcaacgaaa tggaacacca aggcttcagt tcgtcgttga tacgtggcct
     8821 tgttaccagc taccgcaaga ttaatgaaaa ataatagtcg tgaacatgct tgctctacaa
     8881 ggaagcgatc ttttactttc cgatacaaaa tataacttaa cttgacagtc aatgagttat
     8941 aaggtttggg caacaaatag gcaacaaaat ctgtaaaatt gatggcaaat aagagaaaat
     9001 gcctttgttt aattaaaacc tatctacgag caaatataat ccttttttcc ttgttttagc
     9061 tctcaaattg agttcttgat actttggtaa ttcggagcaa taaagtttat cataaggctt
     9121 tgcaaagcca ttcttgacca ttatttcgtt taaggtgttg ccgttaggca ataccacata
     9181 agccaaagtt ctgccgtatg ggtcttgctc gttgcctact tcttgaatta atgtaatccg
     9241 ctttccaatc tttgctttag ttcttaaaaa ttcataagac tggtgtgcaa gctccatcaa
     9301 aaaacgtcct gataaatggg tttctctttc gtcctgcttt agttttttgc aaggtgtcaa
     9361 ctctggtgca tcaattccat aaaaacgtat ttcttcctct ttatttgaga taatattctt
     9421 tgcgattata ccatcaccat cttccacttt tactatttcg tagttcgttt tgataatggt
     9481 ctgacccttt acatctaatt gcttcatatt gcactattta agtaattgat taacagttat
     9541 ttatggcgta aatttacaat aaatttttaa cataaaatac gtattattat ggcacgacaa
     9601 aaaggacata ttaaatatgt cggaacactc ggagaagtta gacacttcaa aattaaaggc
     9661 aaagatggtt actttgcagg tttaaaaggc ggaccatctg ccgaacaaat tgcaacagat
     9721 ccagcctttg ttcgtactcg tgagaatatg aacgaatttg gtggaagtgc aattgcaggt
     9781 aaatccctta gaacaagtat tgcaagttta atccgtaata atggcgatag ccaagttact
     9841 ggaaggataa ccgcaataat gaaaagaatc aatctggaag atggctcaga agcaaggggt
     9901 caaagagcaa ttttggtaac tgttgcacct cagtacttag aaggatttga gtttaacaaa
     9961 ttcacttctt tcaatggtgc ttttaacgct ccttatacca ttactccaac agccacaaga
    10021 gatagttcaa ctttggatat tccttcgttt aatcctttgg atagaatgta tattcctgct
    10081 ggtgctactc atttcaaaat catcaatgct attgctgtgt tgtcggattt tgaatataac
    10141 agtactactg gagtttatga gccaaaagac ggcactacaa acggcttaac agatgtgcaa
    10201 gattcaggtt atttacctgt aaatgctgca actggtgttt ttagtattgt tagtactttg
    10261 cctggttctc ctacaatttc tgcggatgtg tcggttgtga atgtgttggc ggtggaattt
    10321 tatcaggaag taaactcaaa ctactattta ttttctcaag gtaacgcaat gaaagtagcc
    10381 aagatattct aattcttcat cagtgaatta gatgatttcg gaaaatcggg aaagcctgct
    10441 ctaatcgagt aggctttttt ttcgtttgca ccctttttcc tataatggcg aaatatggtt
    10501 taaataagtg tac
//
LOCUS       DAOQQY010000066        10486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2304_length_10486_cov_5.229892, whole genome shotgun sequence.
ACCESSION   DAOQQY010000066 DAOQQY010000000
VERSION     DAOQQY010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10486
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2304_length_10486_cov_5.229892"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="PLE23_04950"
     CDS             complement(<1..1232)
                     /locus_tag="PLE23_04950"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HMX82647.1"
                     /translation="MILDNENGNLKVHEWISKYTKTGKLSIVTGYFTVGALAYFSKET
                     KDKIDEYKFILGDIVNFDSDKDRALDLLNEEINIDASLKLSKVAQEAVAFLELDKVKA
                     KTLEPNFCHAKAYLYKHNDKDPQKDYYISGSSNLTEAGVGLKTTNNVELNIGSFGSDP
                     QYNELIKWFDSLWSRPQAHDYKTVVDGNGGVNRIPFKKYLIDEIKKIFTEYSPKQLYF
                     KVLFELFGDELLLEKDNPEFNRQIGRLENTVVYNSLYEFQQKGVLSLIKMLQKYNGAI
                     LADAVGLGKTWTALAVMKFYQLQGREVILICPKKLQYNWRIYQKNQNSKFEKDQFEYF
                     LRFHTDLTEDLMENYHDRADKLFINEKPKLFVIDESHNLRNDKSKRYKFLVDQILSQN
                     EDVKVLMLSATPINNSLM"
     gene            complement(1446..1775)
                     /locus_tag="PLE23_04955"
     CDS             complement(1446..1775)
                     /locus_tag="PLE23_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HMX82648.1"
                     /translation="MQDNIAEIQEKFKKTDERMHQMQNDIEFFESFLSRYAEAVKNIA
                     EVEEFYFSDSYMQEMEILRNDNLDQYWSTSEDGIWNLGIEYRSVRIKLLKMLTDSIYN
                     ETLLIDK"
     gene            2028..2903
                     /locus_tag="PLE23_04960"
     CDS             2028..2903
                     /locus_tag="PLE23_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HMX82649.1"
                     /translation="MASGIFALLDDITVLLDDIASMSKVAAGKTSAILGDDLAVNAEK
                     ASGFSTSREIPVLWAITKGSFLNKVIILPLAFLLSAFLPSVITIVLIAGGLYFAYEGV
                     EKIVHFFLGHNHESSKEITADMNEAQLLEYEKSKIKSAIITDFILSVEIIVIALSTVM
                     NERLVVQIGVVSVIAILATVGVYGVVALIVRMDEAGFYLIRKSKNQNGLLPKTGWALV
                     NSLPYVIRALAVIGTLALVLVAGGIFAHNINVFHDFLPQLPVLLSEFILGLLIGLAAV
                     LLFQTGSFLYKKIRN"
     gene            3350..4081
                     /locus_tag="PLE23_04965"
     CDS             3350..4081
                     /locus_tag="PLE23_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HMX82650.1"
                     /translation="MDFNQQNYGFTNEPRSLIFHSEEEQNLHVSKYMTKVYGWMFLAL
                     MVTALASQYVASSETLLNFILGNKLGFWVVLLGQLGLVFYMTARIDKISVSTATALFF
                     TYAALNGLVFGVILMAYTPASLLTVFAITAGTFGAMSAIGYFTKADLTSLGQIMFFGL
                     IGLIIASVVNFFLKSSALYWILSYVGVAVFMGLIAYDTQKIKAYALHENEEIRQKGAI
                     MGALALYLDFINLFIYLLRFFGDRR"
     gene            complement(4180..4782)
                     /locus_tag="PLE23_04970"
     CDS             complement(4180..4782)
                     /locus_tag="PLE23_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82651.1"
                     /translation="MKTFFIRILVVILFLSGAEVVQAQGMQGAVTGSVGFLVPKFRFG
                     YESPLGVHGGIGLLANLYVGTWQGIYAEPFIRFYPKDNSTGFFGQFKAGFGSFKVYDY
                     ESFDGGLTHSHWTASGYGMGFGHKYYDSGNFIVETYFGFRYISQPYYKYKNGTVDNVG
                     KYGSSLNWKVGPGFPFQVYIMVGYQFRKKAPDTDRPVYEL"
     gene            complement(4977..6044)
                     /locus_tag="PLE23_04975"
     CDS             complement(4977..6044)
                     /locus_tag="PLE23_04975"
                     /inference="COORDINATES: protein motif:HMM:NF017495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HMX82652.1"
                     /translation="MKSVSVLASISPVLLCYACGIILANIPGYPVNEKQVTQILELAV
                     ILAIPMLLFNSKIQDWKNLAGKSLLAFVLAIIAVLISVLVFPLLFRDLPDVALMSAML
                     TGVYIGGTPNMQAISIALEADNNLFIKVNAAEVASGGIYLLLLAAGFRNVVLRILKPY
                     FIYKVGNEAEPIFNDPHGKVKPMDALKNILISLALTALVLLITKMLYGDLKHSIFIMI
                     AISTLSLIASLFPRINGLPGSYPTGEYFLLIFCVGIGLMADFREILTGSLEILLFAGG
                     MLIMIILVHITLCYLFKIDADNMVISSTATIFGPAFIGQVTGILKNKELLVPGMIMSL
                     IGLAIANYLGVMVAQLLAGLM"
     gene            complement(6230..7996)
                     /gene="sppA"
                     /locus_tag="PLE23_04980"
     CDS             complement(6230..7996)
                     /gene="sppA"
                     /locus_tag="PLE23_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMX82653.1"
                     /translation="MRQFFKFVFASCLGIFLFFIVCFLILMLIGLGSSGDSFKLAQNS
                     VLQLKFDKPIPEKTNNLPFQFSGFSKADNEILGMNDILEAIRHAKTDKKIKGIMLDIS
                     GFQGGFAKAASIRHALKDFAGAGKFVMTYGDYYSQGSYYLSSVATKVYLHPIGMVDLR
                     GLGSQIPFMKDFLDKIGVKAQVFYAGKFKSATEPFRFSKMSEENRYQIKEYLGDLNTN
                     MMRDIAESRKLSLSELMASVNAFDGHDPDLSLEKKLVDKLGYYSDMIAEMKSKIGLKP
                     EEKLNIASLSDYVTTYSSSEDFSSKDKIAVVYAEGEIKDGKTEYGEIGSKNYASIFKK
                     IAEDKNVRAIVLRVNSPGGSAVASETILHEIQNAKSKGIPVVVSMGDFAASGGYYISC
                     AADSIFAEPNTLTGSIGVFSVFPNVQEMMNKKLGINFDTVSTGKYANAFSPFFLLNQD
                     ESRIMQRFTDKIYNVFLQRVAKARKSTTAQINEVAQGRVWTGQDAVGVNLVDKLGSLD
                     DAIACAKRMAKLKNYRITEYPKIKDPITQIIDELSGVKDDQQEKAIRAHLGVYYEYYG
                     QLKKLNEMRGPQMRMPFDLNIR"
     gene            8210..>10486
                     /locus_tag="PLE23_04985"
     CDS             8210..>10486
                     /locus_tag="PLE23_04985"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMX82654.1"
                     /translation="MRKFINFFYTCSLIITSVLWSTSHIHAQISVVSGGIPPVDLIKN
                     VFLGEGVEITSVQYNGSSDAVGYFGNGLKDVQIDKGLVMSSGKAISAATANTSTSTSG
                     QTSGNSVPDPDLVALTNGGNVNDVSQYIIKFKPNADTLRFKYAFASEEYPEFTCTGFN
                     DVFGFFISGPGINGPFSNNAINIALVPDPMDPKGLTFTNIPVAINNVHNGNPFNASCT
                     PQYPMYYNDKTGSTTITYDAVLNVFIAQVIVTPCEEYTIKLAIADKGDSSYDSAVFLE
                     AKSFGTGAVRVTTITTSLDGAIAEGCDKGYFKLKLPKKAPKDTTINYEVIDDPSLATN
                     GTDYTFIAPNQIIQAGDSELVFIIEAFEDGLAEATEPIAIAVQRDFCNLDTFYLYIKD
                     NVLKPPVLPPLSQICETDSVLVDGTLPIQLPAESIFENNIVLNIPDPDTPGGQGIPVF
                     SDIMVNGVIPTTLGPGMISKVCLNVMHSWIDDIDIFLVSPGGQFMELTTDNGRNGKNY
                     TSTCFVPTATQPINYGDPFGAPAIYAPFTGDFMPEGDWNYLYQGGANPTNGVWKLLLI
                     DDTFGIGGKLLNWSITFKPIYEINYAWTPGNLTSCATCAETNLKPSTSQQFILNVTDS
                     YGCVVSEKTQVDVIDKIPAPDVKCGTITANSIDFTWTADPAATGGYLISFNGVNWLAP
                     LPGPTNHTVLGLSLNETVTMYVKIAGLCDGYIDTVVCQTPNCVPPTVNVESISNAKCF
                     GSQDGSIDLSATGQYPPFT"
BASE COUNT         3054 a         2207 c         1904 g         3321 t
ORIGIN      
        1 tccatcaatg aattattgat tggcgttgct gaaagcatca gcacttttac atcttcattt
       61 tgtgaaagaa tctggtcaac aagaaatttg tatcgttttg acttgtcgtt tcgcaaattg
      121 tggctttcat ctatcacaaa cagtttaggc ttttcattga tgaaaagttt gtccgccctg
      181 tcgtggtaat tttccatcaa gtcctccgtt aaatcggtat ggaatcttag gaaatattca
      241 aactggtctt tctcaaattt tgagttttga ttcttttgat agattctcca attgtattgc
      301 aatttctttg ggcaaatcaa aatcacttca cgcccttgca actgatagaa tttcatcact
      361 gccaaggcag tccaagtttt acccaaacct actgcatcgg ctaaaatggc tccgttatat
      421 ttttgaagca ttttaatcaa actcagaacg cctttctgct gaaattcata aagcgaatta
      481 taaacgactg tattttcaag tcgtccgatt tgtcggttaa actctggatt gtctttttca
      541 agcaggagtt cgtcaccaaa caattcaaaa agaactttga aataaagttg tttgggtgaa
      601 tactcggtga aaatcttttt gatttcgtca atcagatatt ttttaaatgg aatacgattt
      661 actccaccgt tgccgtcaac aacggtttta tagtcgtggg cttgtggtct tgaccacaag
      721 ctgtcgaacc attttattaa ctcgttgtat tgcgggtctg aaccaaagct gccaatgttc
      781 aattctacat tatttgttgt ctttaaacca acgcccgcct ctgtcaaatt tgaactacct
      841 gaaatataat agtccttttg agggtctttg tcgttgtgct tgtataaata cgcttttgcg
      901 tgacaaaaat ttggttcaag tgtcttagct tttactttgt caagttccag aaatgcaact
      961 gcttcttgtg caaccttgct tagttttaaa gatgcgtcaa tgtttatttc ttcatttagc
     1021 aagtctagtg ctctgtcctt gtctgagtca aagtttacga tgtcgccaag tatgaatttg
     1081 tattcatcaa tcttgtcctt tgtttctttt gagaaataag ccaaagcccc aaccgtaaaa
     1141 tatcccgtta caatggagag ttttcctgtc tttgtatatt ttgaaatcca ttcgtggact
     1201 tttaggtttc cgttttcgtt gtctaatatc atatggttaa tttattcatt ctgattgtat
     1261 gcccttatgc gttggcaaca aagggctgtt ttcacctgtc aaaatggttt actttttatt
     1321 gcttcagtca gttagtttct gttgggtctt tgtcttttta cacttgcacc caacaattca
     1381 ctacaagtac attacgcttg tgggttactt atcattttcc tgtacttttt ctagttcttg
     1441 tctttctatt tgtctatcag tagcgtttca ttatagatac tgtctgtcag cattttcagt
     1501 agtttgatcc tgacagaacg atattctata cccaagttcc agatgccgtc ctcgctcgtt
     1561 gaccagtatt ggtcgagatt gtcattccgt aggatttcca tttcttgcat atacgagtca
     1621 ctaaaataaa actcctctac ctcggctata tttttgactg cctcagcata tctcgacaag
     1681 aaggattcaa aaaattcaat atcgttctgc atttgatgca tcctttcatc cgtttttttg
     1741 aatttttcct gtatttcagc aatgttgtcc tgcatagtct ttaagcttta gttgagcaaa
     1801 gataaatcac atctccgact atggctgatc attttcacgt atgtgcctca ttttgagcat
     1861 ggttgtataa aagtaacgat tcaatttcat tgcaatacat ttttttcaat accgtgtaaa
     1921 agccattagg gttcgttcca ttttggttat ttttattttt tttgaaattg tccatttaaa
     1981 atttctcttg tcaatgtata tctttgggtg gaatattaaa aaatattatg gcttcaggca
     2041 tttttgcatt acttgatgat attacagttt tattagatga tattgcttct atgagcaagg
     2101 ttgcagcagg aaaaacttct gcaattctag gtgatgatct ggcggttaac gctgaaaaag
     2161 cctccgggtt ttctacatca cgtgagattc cggtattatg ggcaatcact aaaggatcgt
     2221 tcctaaacaa agtcataatc ctgccgttgg catttttgct gagtgcattt ttgccctcgg
     2281 ttatcaccat tgtattaatt gcgggtggat tgtattttgc ttatgaaggt gtagagaaaa
     2341 tagttcattt ctttttgggc cataaccacg agtcaagtaa ggaaataaca gccgacatga
     2401 atgaagcaca attacttgaa tatgaaaaga gtaaaatcaa atcagccatt attacagatt
     2461 ttattttatc agtagagatt attgttattg ccttatcaac tgttatgaat gaacgtttag
     2521 tggttcagat tggtgttgtt tctgttattg caatactggc tacagtaggg gtttacggtg
     2581 tggttgcctt gattgtcaga atggatgagg ccggatttta tcttatccgt aaaagtaaaa
     2641 accaaaatgg gttgctccca aaaacaggat gggcattggt aaattcgctt ccgtacgtga
     2701 tacgagcact ggctgtaatc ggaacactcg cactcgtatt ggtcgctggt ggtatttttg
     2761 cacacaacat caatgtattt catgactttt taccgcaatt gcctgtatta ttaagcgaat
     2821 ttattcttgg ccttttgatt ggacttgccg cagttttatt gtttcaaacc ggctcatttt
     2881 tatacaaaaa aatccgcaat taaaactgat cataatacat ccgactctct atcaacaatc
     2941 ctggtgtgca tataatgaac ccatgtttat aatctttgcc tttcatccaa accggtaaaa
     3001 atgaatgggc gaatgccaat tctacaatac ttgttcgggc agacgaatgg gtaaaaaact
     3061 tatgcggact aagagtagaa taaaggtata acgatgtaga aagttcggat attggttttt
     3121 gaagcaattt tcggcatttt cccgatatta cttaaagaat cacatggctt tttattcttg
     3181 ccattgatgg gtactggtca accagttact catacaaagt taaacccaaa ttttatcgcc
     3241 actattgttg tatatttttt atctggaatt attcctctca acaaatttta tttcaacatt
     3301 aacagaactt ttttcactac ttttgccttt tatatataaa tattataaca tggacttcaa
     3361 tcagcaaaat tacggattca ccaatgaacc ccgctcgctt atttttcatt ctgaagaaga
     3421 gcaaaacctg catgtatcaa agtacatgac caaagtatat ggctggatgt tcctggcatt
     3481 gatggtaact gcactggcaa gccaatatgt tgcatcaagt gaaactttgc tcaactttat
     3541 cctgggcaat aaacttggtt tttgggttgt gctgcttggt caactcggcc tcgttttcta
     3601 tatgacagcc cgtattgaca aaatcagtgt ttctactgct acagctctgt tctttacata
     3661 cgcagcactc aacgggctcg tctttggtgt catcctcatg gcttacactc ctgcttcgct
     3721 gctcaccgtg tttgccatta ctgcaggcac ttttggtgcc atgagtgcca ttggttattt
     3781 tacaaaagct gaccttacca gcctcggaca aatcatgttt ttcggactca tcgggcttat
     3841 tattgcatct gttgtaaatt ttttcctaaa aagctccgca ctttattgga tcttatcata
     3901 cgttggtgtt gccgtattca tgggcctcat agcttacgat actcagaaaa taaaagctta
     3961 tgctttgcac gaaaatgaag aaataagaca aaaaggtgct atcatgggtg ctcttgcctt
     4021 gtaccttgat tttataaatt tgtttattta tctgcttcgt ttctttggcg acagaagata
     4081 atcattcaaa tacctaccat cagaaaatca ggcacatcag ataaagcatc cggtgtgcct
     4141 gattttttat agctttatag aaatcatgta ctacttctgt tacaattcgt aaaccggtct
     4201 gtcggtatca ggtgcttttt ttctaaactg gtagcccacc atgatataaa cctgaaacgg
     4261 aaatccgggg ccgaccttcc agttcagaga gctaccgtat ttacctacat tgtcaactgt
     4321 tccgttcttg tacttgtaat acggttgcga tatgtaccgg aatccaaaat aggtctccac
     4381 gataaaattc cccgaatcat aatacttgtg tccgaagccc atcccatacc ctgaagctgt
     4441 ccaatgcgag tgtgttaatc caccatcaaa tgattcataa tcatatacct taaaactgcc
     4501 gaaacctgct ttgaactggc caaaaaatcc ggtagagttg tcttttggat aaaaacgaat
     4561 aaagggttcg gcatagatgc cctgccatgt accgacatac aagttcgcta aaagtccgat
     4621 gccgccatga acgccgaggg gagattcata cccaaacctg aatttcggaa ccaagaatcc
     4681 cacactgccg gtaacagcac cctgcattcc ctgtgcttgt acgacttcag ctccgctcaa
     4741 aaacaggatg actaccaaga tacggataaa aaaagttttc ataccagtcg attttatctt
     4801 tcaataatgt aaccgggcaa actcagatgg gtttccagat ttttttcttt ctttattcaa
     4861 acaaatataa gagagtttca tttagattca aaatatttgg cgatgctttt aaataaaaga
     4921 atttatttaa taacattgtt tctacagttt aaaaaacttc tccggcaaaa tccgcactac
     4981 atcaatcctg ccaaaagctg tgcaaccatt acaccaagat aattggctat tgccaagcct
     5041 atcaggctca taatcatacc gggcaccaat aactctttat tcttcagaat acctgtgacc
     5101 tgaccgatga atgccgggcc aaaaatagta gctgtactgc ttataaccat attatcggca
     5161 tctattttaa acaaataaca cagtgtgata tgtaccagaa taatcataat caacatacca
     5221 cctgcaaata acaatatctc aaggctgccg gtcaatatct ctctaaaatc tgccataagt
     5281 ccaatgccca cacaaaagat caaaaggaaa tactcaccgg taggataact gccgggtaaa
     5341 ccattgatac gaggaaaaag tgaagcaatt aagctgagcg ttgaaatggc aatcattata
     5401 aaaatggagt gtttcaggtc accatacaac attttggtaa ttaataaaac caatgctgta
     5461 agagccagac ttatgaggat attcttcaat gcgtccatcg gcttcacttt tccgtgtggg
     5521 tcattaaata tcggctctgc ttcatttcct actttgtata tgaaatatgg tttgagaatc
     5581 cgcagaacca cattgcgaaa accagctgcc agcaacagca gataaatacc gccacttgct
     5641 acctctgctg catttacttt aataaaaaga ttattgtcgg cctccaatgc tatgctgata
     5701 gcctgcatat tgggcgtgcc tcctatatac acacctgtaa gcatagccga catgagagcc
     5761 acatccggca aatctctgaa caacaatgga aacacaagca ccgagatcaa tactgctatt
     5821 attgcgagta caaaagccag caaggacttc ccggccagat tcttccagtc ttgtatttta
     5881 ctgttgaaca acaacatcgg tatagcgagt atgacggcca gttcgaggat ttgggtaacc
     5941 tgcttttcat tgacaggata tccggggatg ttagccaata ttattccaca ggcataacac
     6001 aataataccg gactgatgga tgcaagtacc gacacagatt tcaccaatct aatagcgcaa
     6061 aatggcagca ctaccattaa tataatattg aaaatatcta acaccattgt ccttacttaa
     6121 aggataaaag taaaaattac tttattaatt caggtaaaaa tatttataaa aagaaaaaac
     6181 gcctcccgaa aattattcct gaggcgttgc tgtattcaaa attacttatt tatctaatgt
     6241 tcagatcgaa gggcatccgc atctgtggtc cgcgcatttc attcagtttt ttgagttgtc
     6301 cgtagtattc ataatatact ccgagatgtg ctcttatagc cttctcttgt tgatcgtctt
     6361 tcacaccgct tagttcatca ataatttgag taatcgggtc ttttattttc ggatattctg
     6421 tgatgcggta gttcttgagt tttgccattc gtttggcaca ggcaatggca tcgtcaagtg
     6481 accctagttt gtccacaaga ttgacaccta cagcatcctg accggtccat acacggcctt
     6541 gtgctacttc gttaatctga gcggtggtag atttccttgc cttggctact ctttgcagaa
     6601 atacattata tatcttatcg gtaaaacgct gcataatcct tgattcatcc tgattaagca
     6661 agaagaacgg actgaaagca ttggcatact tgcccgtaga aacggtgtca aagttgatac
     6721 caagtttttt attcatcatc tcctgtacat ttgggaaaac cgagaatact ccaatagaac
     6781 ctgtaagtgt attgggctct gcaaatatgc tgtctgcagc acatgatata tagtatccac
     6841 ctgaagctgc aaaatcaccc atacttacta ccacaggtat gcctttactt ttagcgtttt
     6901 gtatttcatg caagatagtc tctgaagcta ccgcacttcc tccaggcgaa ttgaccctca
     6961 gtacaatggc acgtacgttt ttgtcttctg ctattttctt aaaaatactt gcataattct
     7021 tagaaccaat ttcaccatat tcggtctttc catctttgat ttcaccttca gcatatacta
     7081 cggctatttt gtcttttgat gaaaaatctt cagaactgct gtaggtagta acataatcag
     7141 acaatgatgc tatattgagc ttttcttcag gtttcaggcc aattttggat ttcatttctg
     7201 ctatcatatc agaataatag ccaagcttat caaccagctt tttctcaagc gacaagtcag
     7261 ggtcatgacc atcaaaagca ttgacacttg ccataagctc tgataaagat aattttcgag
     7321 attctgctat gtctctcatc atatttgtat tcaaatcgcc cagatattcc tttatctgat
     7381 agcggttttc ctcgctcatt ttcgaaaacc ggaatggttc ggttgcactt ttaaacttac
     7441 cggcatagaa aacctgtgcc tttacgccta ttttgtcaag aaaatccttc ataaaaggta
     7501 tctgtgagcc aagaccacga agatcaacca ttccgatagg atgcagataa acctttgtgg
     7561 caacagaaga taaataatat gagccttgcg agtagtaatc accataggtc attacaaatt
     7621 tccctgcccc ggcaaaatcc ttcaatgcgt gacgaatgct ggcagctttg gcaaatccgc
     7681 cttgaaatcc actgatgtca agcattattc ccttaatctt tttatcagtt ttggcatgtc
     7741 tgatggcttc aaggatgtcg ttcattccca aaatttcatt atctgcctta gaaaagccgc
     7801 tgaactgaaa tggaaggtta ttggtctttt caggaattgg cttgtcgaac tttagttgca
     7861 atacagagtt ctgggcaagt ttgaaactat cacccgaaga cccaagccct atgagcataa
     7921 gtatcaaaaa gcatacaatg aaaaacaaaa aaatacctaa acaagaagca aaaacaaatt
     7981 taaagaactg tctcataaca aacattttga aatccaaaag taatatagtt gctacgaatt
     8041 aactaaatta ttggattaac agctacaaat tttcgtcaaa tacatattat tttataaaaa
     8101 aataataatt tcaattcaca aaacagttgc ttagcatatc tgtagttaat cagcaataat
     8161 tattaaaata caacgacgtt cactattttt gacttttatt aagttaagta tgaggaaatt
     8221 tataaatttt ttttacacct gctcactcat tattacatct gttttatgga gtacaagcca
     8281 cattcatgca cagataagtg tcgttagtgg cgggatacca ccggtcgatt tgatcaaaaa
     8341 cgtctttctg ggtgaaggtg tcgaaataac aagtgtacaa tacaacggaa gctcagatgc
     8401 cgtagggtat tttggaaatg gattgaaaga tgtccagatt gacaaaggtt tggttatgtc
     8461 ttcaggcaaa gccatttcag ctgcaacagc caatacttcg acaagtactt ccggccagac
     8521 atccggcaac agtgtaccag acccggacct ggtagcgctc accaacggtg gtaatgtaaa
     8581 tgatgtatcg caatacatta tcaagtttaa acccaatgca gatacactac ggttcaaata
     8641 cgcatttgca tcagaagaat atcccgagtt tacatgtaca ggatttaatg atgtattcgg
     8701 attttttatt tccggaccag gtatcaatgg gcctttttcc aataatgcca ttaacatagc
     8761 attggtaccc gacccaatgg acccaaaagg tctaacattt accaacatac cagtggccat
     8821 caacaatgta cataacggca atccgtttaa cgcatcctgt actcctcaat acccgatgta
     8881 ttataacgac aaaacaggta gcacaactat tacttacgat gcagtactga atgtgtttat
     8941 tgcacaagtc attgtcacac catgcgaaga atacaccatc aaactcgcta ttgcagacaa
     9001 aggtgacagc agttatgatt cggctgtgtt tcttgaagca aaaagttttg gtacaggtgc
     9061 tgtaagagtt acgaccatca ctaccagtct tgacggtgcc atagctgaag gttgtgataa
     9121 aggttatttt aaattaaaat tgcctaaaaa agctcctaaa gacactacca ttaactacga
     9181 ggtaatcgat gaccccagct tagctaccaa tggcacagac tatactttta ttgctcctaa
     9241 ccagataata caggcaggtg acagtgagct tgtatttatt atagaagcat tcgaagacgg
     9301 acttgcagaa gccacagaac ccatcgccat tgccgttcaa cgcgacttct gtaatcttga
     9361 cacattttat ctttacataa aagacaatgt tctcaaacca cctgtattac caccactcag
     9421 ccagatatgc gaaacagact ccgtacttgt agatggcact ctgcccatcc agttacctgc
     9481 agaatctatt tttgagaata acattgtact caatattcct gaccctgaca caccaggcgg
     9541 tcagggcata cccgtttttt cggacatcat ggtcaatggt gttataccta ccacgctggg
     9601 acctgggatg atctccaaag tatgtttaaa tgttatgcat tcatggattg atgatattga
     9661 catatttctg gtatcaccag gtggacaatt tatggaactg actaccgaca acggccgtaa
     9721 cggcaaaaat tatacaagca cctgctttgt accaacagcc acccagccaa ttaactatgg
     9781 cgatccgttt ggtgcacctg ctatttatgc acctttcacc ggggatttta tgcctgaagg
     9841 tgactggaac tatctgtatc agggtggtgc caatccgacc aatggtgttt ggaaacttct
     9901 cctgatcgat gacacatttg gtattggcgg gaaattgctc aactggagca ttactttcaa
     9961 acctatttat gaaataaact atgcctggac ccctggcaac cttaccagct gtgccacttg
    10021 tgccgaaacc aacctcaaac catcaacatc acagcaattt atcctaaatg ttactgacag
    10081 ctatggatgt gtagtgtcag aaaaaaccca ggtagatgta attgataaga tacctgcacc
    10141 ggatgtcaag tgcggcacta taacagccaa ctctattgac tttacctgga cagccgatcc
    10201 ggcagctacc ggtggttacc ttatcagttt taatggcgtc aactggcttg ctccactccc
    10261 cggccctact aaccacactg tacttggcct gtcgctgaac gagaccgtta caatgtacgt
    10321 aaagattgcc ggcttatgtg acggatatat cgatacggtt gtatgtcaga cgccaaattg
    10381 cgttcctccg acagtcaatg ttgagagcat ttccaatgct aaatgctttg gtagtcagga
    10441 tggttcgata gatctttctg ctaccggcca atacccacca ttcact
//
LOCUS       DAOQQY010000067        10480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2306_length_10480_cov_5.756739, whole genome shotgun sequence.
ACCESSION   DAOQQY010000067 DAOQQY010000000
VERSION     DAOQQY010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10480
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2306_length_10480_cov_5.756739"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(150..1031)
                     /locus_tag="PLE23_04990"
     CDS             complement(150..1031)
                     /locus_tag="PLE23_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82655.1"
                     /translation="MKKVLFGFLLIGLMTSCVSKKKFTALQSEKDQLMQMLENNKKLL
                     ADCNDEKAKLTADLSAKGQEAANATKQIKSLEDQIASLNNNNTNLLKRLEDLSIISQA
                     GAESIKKSLDAMNEKDKYIKNLTSSMARKDSLNLALVMNLKKSLADVNDTDVQIEVKK
                     GVVYISISDKMLFQSGSSMINASAQNVLAKVAKVLNDHKDLDILVEGHTDNVPISTED
                     IKDNWDLSAKRATAVVRTLQNKYKVDPSRLTAGGRSEYSPKTSNATTEGKKLNRRTEI
                     IILPKLDQFFQMLAPSK"
     gene            complement(1121..2527)
                     /locus_tag="PLE23_04995"
     CDS             complement(1121..2527)
                     /locus_tag="PLE23_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HMX82656.1"
                     /translation="MKRLLCICSFVGFLLSLEAQTPDYITNQYDYTVTKDISYGSDTA
                     YDGKLTELFLDLYKPVGDNNCKRPVIVLLHGGAWIGGDKAEGQIPAVAAQFAAKGWVV
                     ASINYRLGMHTAKGYTQYLLCPTDNPALCAHVCDSSEIFRAIYRGMQDTRGAIRFLKL
                     RAESDSTDVANVHVAGESAGAFLAMYTAYLNRDEEKPSECYELPDAPTSAPAFNFCHK
                     APKSLARPDLGSIHGSLHLGEANDSVQGVACFYGAMHDLSIMDDNLMPTYIFHQASDV
                     IVYYKRSRLLNRLSDCLSSICQPYYNMPLMSGGFLIKKHLSDLGTSAPPYKADILENS
                     TAFDCTINPPGHGIDNIPLRTKNIAELFAPIVQTNGNNPADNCQTTHITTYSSTHLRI
                     FPNPGTGEFILSTPDEMEVTINMTDVLGRQTNIITNKISNNAYKVIFMENAHPGIYNC
                     NIISPNNKWSTTYIYNNY"
     gene            complement(2524..3489)
                     /locus_tag="PLE23_05000"
     CDS             complement(2524..3489)
                     /locus_tag="PLE23_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX82657.1"
                     /translation="MKNLLALLLLSFLTTTLSFCQTRPAPYVHPEGKYYTINGAKIWT
                     ETLGNGHPLFLIAGGPGGSHVGLHSFDPLADNHKLIYFDGFGRGKSDLAKSPTEYSLQ
                     RDVEDLEQLRIAIGLGKIDILGHSYGSVVGQAYAIKYPHNVSHLIIANGFHSYLMWQK
                     NCDNSNHEIKTNYPEVWDTLMTVREQGFVSADPIHQEIYGRVPYGFLYGYNPDKFRGP
                     SRFSDYPNRMNTELYYQMVGKDGDFVVTSDIGTFDFRRDIGKLYMPILVIAGRYDRVA
                     VPSEMVKYKNFCPKCEYVTFERSGHNPQVEEPEKEFNLINEFLAK"
     gene            3808..4116
                     /locus_tag="PLE23_05005"
     CDS             3808..4116
                     /locus_tag="PLE23_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82658.1"
                     /translation="MRGRAIFLLFIFTSFTVLATVNTVFIGNNNLSLVSAQNLPEEEH
                     QHGGEHGAKGKMECSIGVLALDLINFENRLENIFKELSLLYEHRTGEIIIPPPELLLV
                     "
     gene            4232..>4509
                     /locus_tag="PLE23_05010"
     CDS             4232..>4509
                     /locus_tag="PLE23_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMX82659.1"
                     /translation="MSKNKILANLKYDFPSGLVVFLVALPLCLGIAMASGAPLFSGII
                     AGVVGGIVIGSLSNSHISVSGPAAGLTAIIISSISTLGSFEAFLTAVF"
     assembly_gap    4510..4520
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4521..5920
                     /locus_tag="PLE23_05015"
     CDS             <4521..5920
                     /locus_tag="PLE23_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMX82660.1"
                     /translation="GVLQLMFGFLRAGSISNFFPNNVIEGMLVGIGIIIILKQIPKAL
                     GSVTEVGNGLSMFGDLVKAMSEVQPGVLFISLVSLSVILLWDKVGPLLKMNFLPSALL
                     AVICGTLLNELFINAGSPLAITSSEYLVELPVVHDMSDILGIFIFPDFINTENGINFS
                     VLLKKEIWVVAATIAVVASVETLLCIEASDRMDPQKRLTDTDLELKAQGVGNMISSLI
                     GGLPMTSVVVRSSANKNAGARTKLSTIVHGMLLLVSVMTIPTILNRIPLSVLAAILLV
                     IGFKLAKPSVFRHFMQRGKYQAVPFLLTVVAVVFTDLLTGVALGMGMSIFAILRGNMK
                     RAYYFRKDGYHEGDVIHIHLAQEVSFLNKAAILFTLDALPNHSKVIINASDTVYIAHD
                     ILDSIREFKEIRAALRNIDLVLVGFKEEYQLENTNTDIRQVSIEHDMIIKHRKRHKVS
                     STVLREFKSIQPDVV"
     gene            5954..6601
                     /gene="can"
                     /locus_tag="PLE23_05020"
     CDS             5954..6601
                     /gene="can"
                     /locus_tag="PLE23_05020"
                     /inference="COORDINATES: protein motif:HMM:NF007756.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HMX82661.1"
                     /translation="MSEFYKKIIENNRNWVASKLSEDPEFFLKLKDAQKPPLLWIGCS
                     DSRVPANEIIGTKPGEVFVHRNIANMVIHTDMNMLSVLDYAVNVLKVRHIIVCGHYGC
                     GGVKAAMSNNSVGLIDNWIRHIKDGYRFSKDEIQSISDEEKKFDRLVELNTKQQVLNL
                     AVTSIVQCAWSKGQELSIHGWVYGLDTGLVQDLDVTFSSHEDIRNSSVYKLEFDE"
     gene            6715..7029
                     /locus_tag="PLE23_05025"
     CDS             6715..7029
                     /locus_tag="PLE23_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HMX82662.1"
                     /translation="MTTKIAALRFLALAEGISYLAFGLTMPLKYIYEITGPNKVIGYA
                     HGALFIAYCVAVFLVNMQAKWNFKTNFLAYLASLVPFGTFVADTRIFKPTEARLISKM
                     QP"
     gene            7109..8788
                     /locus_tag="PLE23_05030"
     CDS             7109..8788
                     /locus_tag="PLE23_05030"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMX82663.1"
                     /translation="MPRLLLLLVMLGILPVTDYAQFIQTTTEKFTRADTLRGSLRPER
                     NCYDVIHYNIDLELLVADKAITGFVDMRFKVKNDFNRLQIDLFDNMIIDSILLGGQKC
                     TYTREFNAVFVDVPKQHSGTEATLRTYYQGRPVIAKNAPWDGGFVWKTDTNKNPWVAV
                     ACEGTGASLWWPCKDHPADEPDSLSFSMTVPHSLTCISNGNLIGKTPLGDGKNKFHWK
                     VTYPINLYNVTFYAGDYYLIDDEYLDFKHRRLDIDYWTLGEKTFKAKLHFRQVDGMLH
                     AYEHYFAPYPYFNDGFALVESPYLGMEHQSAIAYGNQYNRGYLGGRIPDDQNFDFIII
                     HESAHEYWGNSVTADDHADLWIHESFTTYCEALYVEWINNYKKAVDYLVYQKPYIKNL
                     SPMVGPRDVNYNNWKDSDIYYKGSWMLHSLRNTINNDSMWFGYLKAVYNHFAGSVTNT
                     TAVVDFTNQYFNKDLTPFFRQFLMYKELPVFEYFIDKKGKNIDLYYRWNCQVKEFDMP
                     QKVTNGREVFRIFPTGKWQKHRLKDFSVENFKLDETSFLMGTYKVTQKPKI"
     gene            8909..9361
                     /locus_tag="PLE23_05035"
     CDS             8909..9361
                     /locus_tag="PLE23_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HMX82664.1"
                     /translation="MYTGMMHLHSTLRYVLLILLIVTIYKAYTGYKQGRAFAAGDDKL
                     SLYTMIVTHLQLLIGLYMYFASPTVQNALTDMKTAMADKVLRFLAVEHILGMLIGIAL
                     ITVGRIASKKKSTGTQKFKTIWLYYGLGLLVIFLSIPWPFRGLGYGWF"
     gene            9452..10456
                     /gene="holA"
                     /locus_tag="PLE23_05040"
     CDS             9452..10456
                     /gene="holA"
                     /locus_tag="PLE23_05040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMX82665.1"
                     /translation="MTFQSIIQDIKSGKISPVYLLHGEEPFFIDQIEDAIEKNVLTES
                     EKAFDQTICYGKDTDVRSLTDLLSKYPMISQRQVVIVREAQELKKIEELAGYIKSPMK
                     STVLVLAHKYKKVDGRTQFAKLAASSGVVFESKPIPDYQIADWIGNLLRDHKINTTPD
                     VQAVLAEALGNDLTKINNEIEKIKLNIPAGTKLDRNLIDKYIGISKDYSVFELNRFIG
                     LRDMPKVMKTVMYFNASAKKNPITVTIANLFSYFNKIYLLHQVRTASETEQAKVLKLG
                     TLFFLKEYKAAAAKYTATQCEKVIGMLREYDLRSKGVDNVSTTHEELLKEMCTKIVMV
                     "
BASE COUNT         2886 a         2028 c         2485 g         3070 t
ORIGIN      
        1 agaagccggc acgaagtaag gcggatagcc tgacccgaca ggggcacgcc caaatgaaat
       61 acaatatact gctctattgg tttgtgtatg taaaaaagcc tgatgaatga tatccatcag
      121 gctttttttg tttgggtcag tgtctgagat tacttgctgg gtgcaagcat ctggaagaac
      181 tgatcgagct taggaagtat gatgatttct gttctgcggt tgagtttttt gccttcggta
      241 gtggcattgg atgtcttagg tgaatactct gaacgaccgc cggctgtgag ccttgatgga
      301 tcaactttgt atttgttctg gagtgtcctc actacagctg ttgctctctt ggcacttagg
      361 tcccagttgt ctttgatgtc ttcggtagaa ataggaacat tgtctgtgtg gccttctaca
      421 aggatgtcga ggtctttgtg gtcgttgagt actttggcta ccttggcaag tacgttctga
      481 gctgaggcat taatcattga gctgcctgac tggaacaaca tcttgtcgga gatagaaata
      541 tacaccacac ctttcttcac ttcgatctgt acgtcggtgt cgtttacatc agcaagtgat
      601 tttttgaggt tcatgaccag ggcgaggtta agagaatctt tgcgtgccat tgaacttgta
      661 aggtttttga tgtatttgtc tttttcgttc atggcatcga gcgatttttt gatgctttcg
      721 gcacccgcct gactgatgat ggataggtct tcaagacgct tgagaaggtt tgtgttgttg
      781 ttgttaagtg aagcgatctg gtcttcgagt gacttgatct gtttggtagc attggctgct
      841 tcctgacctt tggcagacag gtcggcagta agttttgctt tttcgtcgtt gcagtctgca
      901 agtaattttt tgttgttttc gagcatctgc ataagctggt ctttttcaga ctgtagagca
      961 gtgaattttt tcttgctgac acatgaagtc ataagaccga tgagtaaaaa tccgaagagt
     1021 acttttttca tataaatttt ttagtgatgt gggtttataa agtattaacg ttaaaaaaga
     1081 aataatattc tgcaaaggta caaaaacttt ggtactgacg ctaataatta ttataaatat
     1141 atgttgttga ccatttatta tttggcgaaa taatgttaca attgtagatg cccggatgtg
     1201 cattttccat aaatattacc ttgtaagcat tatttgaaat tttgttggtg ataatattgg
     1261 tctgcctgcc taatacatct gtcatgttga ttgttacttc catttcatct ggtgttgata
     1321 aaataaattc gcctgtgccc ggatttggga atatgcgtaa gtgtgtagag ctataagtgg
     1381 taatatgggt ggtctggcag ttatcggccg gattattgcc attggtttgc actatgggcg
     1441 caaacagctc tgctatattt ttggtgcgta atgggatgtt gtcgatacca tgcccgggtg
     1501 gattgatagt acagtcgaat gcagtactgt tctcaagtat gtctgccttg taaggcgggg
     1561 cggaggttcc gaggtcactg aggtgttttt tgataaggaa gcctccagac atgagtggca
     1621 tgttgtaata aggttggcaa atgctgctga ggcaatcgga cagacgattg agcagccttg
     1681 accgcttgta gtaaacgata acatctgagg cctgatggaa tatgtaggta ggcatgaggt
     1741 tgtcgtccat gatgcttaga tcatgcatgg caccataaaa gcaggccacg ccttgcactg
     1801 agtcgttcgc ctcgcccagg tggagtgagc cgtgtatgct gccgagatcg ggacgggcga
     1861 gcgatttggg agctttgtga cagaagttga aggcaggggc agatgtcggt gcatcgggta
     1921 gctcatagca ttcggatggt ttttcttcat cacggttcag gtaggcagtg tacatagcca
     1981 ggaatgcacc tgcgctttca cctgcgacat gcacattggc tacgtcggta gaatcggact
     2041 ctgctctaag cttcaagaaa cggatggcac cgcgagtgtc ttgcatgccg cggtagatgg
     2101 ctctgaatat ttctgacgaa tcgcacacat gagcacatag tgccggattg tcggttgggc
     2161 aaagcagata ttgggtgtag cctttagccg tatgcatacc aagtcggtaa ttgatgcttg
     2221 ccaccaccca acctttggca gcgaactgtg ctgccactgc gggtatctgg ccttcggctt
     2281 tgtcgccacc tatccaggca ccaccatgta agagtactat aacaggtcgt ttgcagttgt
     2341 tgtcaccgac tggcttgtac aggtcgagga acagttctgt cagcttaccg tcataagcag
     2401 tatctgagcc gtagctgatg tctttggtga ctgtgtaatc gtattgatta gtaatgtaat
     2461 cgggtgtctg tgcctcaaga cttagcaaga aaccgacaaa gctacagatg cataaaagcc
     2521 ttttcatttt gcaaggaatt cgttgattag attgaattct ttctctggtt cttcaacttg
     2581 tgggttgtgg ccactgcgtt cgaatgtaac gtattcgcac ttagggcaga aatttttgta
     2641 cttgaccatc tcggaaggta ctgcaacgcg gtcataacgc ccggcgatga cgagtatggg
     2701 catatagagt ttgccgatgt cgcgcctaaa gtcgaatgtg ccgatgtcgg atgtgacgac
     2761 gaagtcaccg tctttgccca ccatttggta gtaaagctct gtgttcattc tgtttgggta
     2821 atctgagaag cgacttggtc cccggaactt gtcgggattg tagccgtaga ggaatccgta
     2881 agggacgcgg ccatagatct cctgatggat cgggtcggct gatacaaaac cttgttctct
     2941 gacggtcatg agtgtatccc agacttccgg gtaattggtc ttgatttcgt ggttagagtt
     3001 gtcgcagttt ttctgccaca tgaggtaaga gtggaatccg ttggcaatga tgaggtggct
     3061 tacgttgtga gggtatttaa tagcataagc ctggccgact acagagccgt aagagtggcc
     3121 aaggatgtcg atcttgccta agcctatggc aatgcggagc tgttcgaggt cttctacatc
     3181 gcgttgcagg ctatattctg tagggctttt agccaggtcc gacttgccac gaccgaagcc
     3241 gtcgaagtag atgagtttgt ggttatcggc gagtgggtcg aagctgtgta ggccgacgtg
     3301 cgagccgccg ggtccgcctg cgatgaggaa gagcggatga ccattgccga gggtctctgt
     3361 ccagattttg gcaccgttga tggtgtaata tttgccttcc gggtgtacgt aaggagcagg
     3421 ccttgtctgg caaaaggaga gggtagtggt caggaacgac agcaacaaga gggcgagaag
     3481 gtttttcatt ttttgtttat ttttgtaaaa ttaagtatta gggttgtaaa gagaggggga
     3541 gataatggta atttttttaa aaattttaag ttttacctgg ttcagatgca gtttttctaa
     3601 tggtagtcgg gtaaaatatt ttgtttcgat ttaaattatt gatatagcct ttgttaaata
     3661 taaaaattgt tgttgaataa accatccggc atcatcaggt gttgtgcagg agagtgtaca
     3721 cacaaatagg gcagaataag cttgtaatga aggcgtattg tttacaatgc ttgcctatat
     3781 ggtaggagaa tattattttt gtatcgaatg agaggaagag ctattttttt attgtttatt
     3841 tttacttcgt ttacagtgct tgctactgta aatacggtat ttatcggtaa taataatctt
     3901 tctttggttt cggcgcaaaa tttacccgaa gaagagcacc agcatggcgg agagcatggt
     3961 gcaaaaggga aaatggaatg ctcgattgga gtattagctt tagatctgat aaattttgaa
     4021 aatcgtctag aaaatatatt taaggaatta tcattgcttt atgagcaccg taccggtgaa
     4081 ataattattc ccccacccga acttttgtta gtgtagaaag tcatagcctt gctgtgatat
     4141 cattttaaca ggatttatca acgttttggc actcaatgag taacagaaag ttggagtccg
     4201 tatttttttt caacattaac attatacaga catgtctaaa aataaaattt tggctaatct
     4261 gaagtatgat tttccttcag gcttagtagt atttctggta gccttgccct tgtgcctggg
     4321 tatagcaatg gcatcaggag caccattgtt ctcgggtatt atagcaggtg tagtaggcgg
     4381 tatcgtcatt ggttctctat ccaattctca tataagtgtt tcaggtccgg ctgcagggtt
     4441 gacagccatc atcatttctt caatttctac tttagggtca tttgaggctt ttctcacggc
     4501 ggtttttttn nnnnnnnnnn ccggtgtgct ccagctgatg tttggtttcc ttcgggcagg
     4561 ttctatctcc aacttttttc cgaataatgt tattgaggga atgttagtag ggataggtat
     4621 tatcatcatt ttaaaacaaa taccaaaagc cctgggttca gttacagagg taggtaatgg
     4681 tctttcaatg tttggtgatc tggtaaaagc aatgtctgag gtacagccgg gtgtattgtt
     4741 catatcactg gtttcattgt cggttattct actttgggac aaggtcgggc cattgctcaa
     4801 aatgaacttt ttaccaagtg cactcttggc agtaatttgt ggaacactac tcaacgaatt
     4861 atttatcaac gctggtagcc cattggctat tacatcatct gaatatttgg tagaactacc
     4921 cgttgttcat gatatgagtg acattctcgg aatatttatt tttccggatt tcataaatac
     4981 agaaaatggg attaatttct ctgttttgct gaaaaaggaa atttgggttg tagcagctac
     5041 cattgctgtt gttgcatctg tcgaaaccct cttgtgtata gaagcatcag accggatgga
     5101 tcctcaaaag cgactgactg atactgacct tgaacttaaa gcgcaggggg tgggcaatat
     5161 gataagttca cttatcggag gtttgcccat gacttcggta gtagtgcggt cttcagccaa
     5221 taaaaatgcc ggtgccagaa caaaactatc taccattgta catgggatgt tgcttttggt
     5281 gagtgttatg actatcccga ccatactgaa tagaattccc ttaagtgtat tagctgccat
     5341 actattggtt ataggtttta agctggcaaa gccaagtgtg ttcaggcact ttatgcaaag
     5401 aggcaaatac caggcagtac catttttact tacagtggtt gcggtggttt ttaccgattt
     5461 gttaaccgga gttgcactcg gaatgggtat gagtattttt gcaattttgc gtggcaatat
     5521 gaaacgggct tattattttc ggaaagatgg ataccacgaa ggcgatgtta tacacattca
     5581 tttagcccag gaggtatcgt tcctgaataa agctgccatt ttgtttaccc tggatgcact
     5641 gccgaatcat tcgaaggtta taatcaatgc atctgatacc gtctatatcg cacatgatat
     5701 tcttgacagc attcgggagt ttaaagaaat cagagcagct ctacgcaata ttgatttggt
     5761 gttggttgga ttcaaggaag aatatcaact cgaaaatacc aatacggata tcagacaggt
     5821 atcaatagaa cacgatatga tcattaaaca cagaaaacga cacaaagttt cgagcacggt
     5881 actgcgggaa tttaaatcga ttcaaccaga tgttgtataa ataaactaaa tttgtaatta
     5941 atcattcaaa attatgtcag aattttataa aaaaataatc gagaataaca ggaattgggt
     6001 agcttcaaaa ttgtctgaag accctgagtt ttttttgaaa ctgaaagatg cacaaaagcc
     6061 accgctgctg tggatcggat gttcggatag cagagtgcca gccaatgaaa tcataggtac
     6121 caagccggga gaagtatttg tacaccgaaa tatagccaac atggtcatac acacagacat
     6181 gaatatgctc agcgtgcttg attatgctgt gaatgtgctg aaagtgaggc acatcatagt
     6241 ctgcgggcac tacggctgtg gtggtgtcaa ggcagcaatg agcaataatt ctgtcgggct
     6301 gatagacaac tggatacgac atattaagga tggttatcgt tttagtaaag acgagataca
     6361 atcaatatct gatgaagaaa aaaagtttga ccgactggtc gaattgaata ctaaacagca
     6421 agtgcttaac ctggccgtga catccatcgt acaatgtgcg tggagcaaag ggcaggaact
     6481 gtccatacat gggtgggtct atggcctcga taccggactt gtccaagacc ttgatgttac
     6541 gttttcgtca cacgaagata tccgaaatag cagtgtttat aaactggaat ttgacgaata
     6601 gctttattgt tagataatga ttacttgggt atgtgtcatg gtattgtcaa aacaaatcgg
     6661 tgccattgcc tctgacttaa acaggtatat ctttgtggca aaaattctaa gattttgaca
     6721 acgaagatag ctgcactcag atttttggca ttggcggaag gtatatcgta cctggctttt
     6781 ggccttacca tgccactcaa gtatatatac gaaatcacag gcccaaacaa ggtgatcggt
     6841 tatgctcacg gtgctttatt catcgcatat tgtgttgcag tgttcttggt caatatgcaa
     6901 gcaaaatgga actttaaaac caactttctg gcttatctgg catcacttgt cccattcggc
     6961 acttttgtag ctgatacaag gattttcaaa ccaacagaag caagattgat atcaaagatg
     7021 cagccttgat gcaggattga atatactgtt ttttactatc tgcctttttg tggaaaattt
     7081 atatttttac aaaaaaatca atcacatcat gccacgatta ctgcttttgc ttgttatgtt
     7141 gggaatattg ccggtaacag attatgcaca atttatacaa accactaccg aaaaatttac
     7201 ccgtgccgat acactgcgtg gctcgctccg tcctgagcgc aactgctatg atgtgatcca
     7261 ttacaacatc gacctcgaac tactggtggc cgacaaggcg attaccgggt tcgtggacat
     7321 gaggttcaag gtaaaaaatg attttaaccg gttgcagatt gacctttttg acaatatgat
     7381 catcgattct atcttgcttg gagggcaaaa atgtacttac acaagggaat ttaatgccgt
     7441 ctttgttgat gtgccgaagc agcactctgg cacagaagcc accttgcgaa cttattatca
     7501 aggtaggccg gtaatagcca aaaatgcacc ctgggacggc ggatttgtat ggaaaacaga
     7561 tactaacaag aatccatggg tggcggttgc ctgcgaaggt acaggcgcca gtctctggtg
     7621 gccctgcaaa gaccatccgg ccgacgaacc agacagtctg agtttcagca tgacagtgcc
     7681 acattccctt acatgtattt caaatggcaa cctcattggt aaaaccccac ttggcgatgg
     7741 gaagaataag tttcactgga aagtgaccta cccgattaac ctgtacaatg taacatttta
     7801 tgccggagat tattacctga tagatgacga atacctcgac ttcaaacacc gccggcttga
     7861 tatagattac tggacactgg gtgaaaaaac ttttaaggca aaactgcatt tcagacaagt
     7921 ggacgggatg ctgcatgctt atgaacatta tttcgcacct tatccttact tcaacgacgg
     7981 ctttgcattg gtcgaatcgc cttacctcgg tatggaacac cagagtgcca ttgcttatgg
     8041 caatcagtac aaccgtggtt accttggtgg ccgcattccc gatgaccaga attttgattt
     8101 tattatcatt cacgaatcgg ctcacgagta ttggggtaat agcgtgacag ccgacgacca
     8161 tgccgacctg tggattcatg aatcgttcac aacctattgc gaagcgctct atgtagaatg
     8221 gatcaataat tataaaaagg cagtggatta tctcgtttac cagaagccgt acatcaaaaa
     8281 cttaagtccg atggtagggc cacgagatgt caactacaac aattggaaag acagcgacat
     8341 ctactacaag ggaagctgga tgttgcacag ccttaggaac accatcaata acgacagtat
     8401 gtggttcggt tatctgaagg ccgtgtataa ccattttgca ggaagcgtta cgaacacaac
     8461 tgcagtagtc gattttacca accaatattt caataaagat ctgacaccgt ttttcagaca
     8521 attcctgatg tacaaagagt tgcccgtatt cgaatatttc atcgataaaa agggaaaaaa
     8581 tatagacctg tactaccgtt ggaactgtca ggtgaaggag ttcgatatgc cacagaaagt
     8641 cacgaacggg cgtgaagtgt tcaggatatt ccctacggga aaatggcaaa aacacaggct
     8701 gaaagacttt agtgttgaaa actttaagct ggatgagacg agttttttga tggggaccta
     8761 caaagtaact caaaaaccta aaatctgatt tgaaagtaaa actgctttgg atgatgaatc
     8821 tttattattt tgccgcaaag gcggaatttc cttcttttat acagaatcat tcttatcttt
     8881 ggcacacaat taaaaaacta taaagactat gtacacaggt atgatgcacc ttcacagcac
     8941 actcagatac gttcttctga tattgctgat tgttaccatt tacaaagctt atacaggtta
     9001 taagcaaggc agggcatttg cagccggaga cgataaactc agcctttata ctatgatagt
     9061 tacccatctt cagttgttga tagggcttta tatgtatttt gcaagcccaa ccgtgcagaa
     9121 tgcccttaca gatatgaaga ccgctatggc cgacaaagta ttgcgctttc tggcagtaga
     9181 gcacatattg ggtatgctca ttggtattgc tcttattaca gtagggcgta ttgcatccaa
     9241 gaaaaagagc accggtacgc agaaattcaa aaccatctgg ttgtactatg gtcttggact
     9301 tctcgtgata ttcctaagca ttccatggcc gttcagaggc cttggttatg gatggtttta
     9361 atcagaaacg ggatgtttct tatctcttga attacgggat acaggcattt tgtatccgtt
     9421 gaggcattgt ttgtattaaa atattcgatt catgacattc cagtcaatca ttcaggatat
     9481 caaaagcggt aaaataagcc ccgtctatct gctgcatggc gaggagccgt tcttcatcga
     9541 tcagatagag gacgccatag agaagaacgt gctgaccgaa tcagaaaaag cattcgacca
     9601 gacgatatgc tacggaaaag atacagatgt gcgctcccta acagacctgt tgagcaagta
     9661 tcccatgatc agccagcgcc aggtagtcat cgtgcgtgaa gcccaggaac tcaaaaaaat
     9721 agaagaactc gccggatata tcaaatcacc catgaaatcg acggtactcg tgttggcaca
     9781 caaatacaaa aaagtggatg gcagaactca gtttgccaag ttggcagctt ccagcggagt
     9841 cgtgttcgag tcgaagccca tacccgacta tcagatagca gactggatag gcaatctgct
     9901 ccgcgaccac aagattaata ccacacccga tgtgcaggca gtgttggcag aagcactcgg
     9961 caacgacctc accaagataa acaacgagat agaaaaaatt aagctcaata tacctgccgg
    10021 aaccaaactc gaccgcaatc tgatagacaa atatatcggt atcagcaaag actactccgt
    10081 tttcgaactc aaccgcttca tcggtttgcg cgacatgccg aaagtgatga aaaccgtcat
    10141 gtacttcaat gctagtgcca aaaagaatcc gataaccgtt acgattgcca atcttttttc
    10201 atattttaat aaaatttatc tgttgcatca ggtcaggact gcttcagaaa ccgagcaggc
    10261 gaaggtactc aagctcggta cactgttttt ccttaaagaa tataaggctg ctgccgcaaa
    10321 atacactgcc acacaatgcg aaaaagtcat tggcatgcta cgtgagtacg acctgcgctc
    10381 taaaggagtt gacaatgtca gcactacaca cgaagaactt ctaaaggaga tgtgtaccaa
    10441 gattgtgatg gtgtaacata cctccatgta ccgtttcagg
//
LOCUS       DAOQQY010000068        10443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2322_length_10443_cov_5.448402, whole genome shotgun sequence.
ACCESSION   DAOQQY010000068 DAOQQY010000000
VERSION     DAOQQY010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2322_length_10443_cov_5.448402"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..856
                     /locus_tag="PLE23_05045"
     CDS             77..856
                     /locus_tag="PLE23_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMX82666.1"
                     /translation="MAIVISLLNHKGGVGKTTSAINIGAGLVEQGKKVLLVDLDPQAN
                     LTLSLGIPRQKNSIYESIRGESELDPYHVKENLDVITSSLDLSGAEMELINEAGREYI
                     LRELFVPVAEEYDYIIIDCPPSLGLLTLNALTSSQYVLIPLQTEFLAMQGLAKIKQII
                     DKVKFRLNKELELGGVIATMYDSRKVLNRDVVDTIIKHFGEKVFKTMIRDNVALAEAP
                     AQRKDIFAYSPRSIGAEDYLNLSKEIIQRTGAEVLAGVESK"
     gene            875..1381
                     /locus_tag="PLE23_05050"
     CDS             875..1381
                     /locus_tag="PLE23_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82667.1"
                     /translation="MKKTIRNKEPLAIGNDLEWTETSEDLAVTTTVALPKKAGKKFFN
                     DLDAFFESAIDDLWKEPVEERLETNQNKEEPVKQDRKGNKKSFFSGLDALFSNTAMPT
                     TVSTEEDLRAFDEETLKRVTFLFKKDKLDALKNMAKKEKLYLRDIISRVLDQYIEEKG
                     ISASKNQK"
     gene            1385..1897
                     /locus_tag="PLE23_05055"
     CDS             1385..1897
                     /locus_tag="PLE23_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMX82668.1"
                     /translation="MAFILPVKGIYPEFGKNCFIAPNATIVGDVIMGDDCSVWFNAVV
                     RGDVNSIKFGNKVNIQDGAVIHCTYQKTITTLGDNVSVGHNAIVHGCQVGNNVLIGMG
                     AIVMDDAVIEDNVLIAAGAVVLEKAHLKSGMIYAGVPAKPVKELTVEQFGHYVERIAN
                     NYTMYADWFR"
     gene            complement(1925..2812)
                     /locus_tag="PLE23_05060"
     CDS             complement(1925..2812)
                     /locus_tag="PLE23_05060"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HMX82669.1"
                     /translation="MAKPHPPKGTLIAVGGNEDKGIEQSEQYSLDFIEDGILSRIVKE
                     AGGKDANISVVTTASSIPQQVAKNYLTAFGKLGCKNVNILDIRNKFDVKKADNINLIK
                     KSDVVMLSGGDQRRLVHIIGGSEIHKILKQRYQEDDFIIAGTSAGAMAMSKEMISGGS
                     STTAMLKGNVSMMQGFGFLEEVIVDSHFINRGRFGRLTEAVAKFPKLLGIGLGEDTGV
                     IIKEGNICTTIGSGMVLVFDGEHLSHNKVPDLDEGTPISIGNMIVHVMANTDTFYLGT
                     RHLKILTADQAYEQEADWQ"
     gene            complement(2911..6381)
                     /locus_tag="PLE23_05065"
     CDS             complement(2911..6381)
                     /locus_tag="PLE23_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82670.1"
                     /translation="MIVGNVSLDAQTSRVEFGKNRVQFHKKFKEWTYYEGDRTIVYWY
                     GDGRNVGLAAAHIAETEMDGIRDLLEHSISDKIEIIVYTDLTDLKQSNIGLEDAFEYS
                     GGSTKILDNKIFLYFDGNHRHLHRLIRQGIAGVYIESILYGSSLQEIVQNAVSFNLPD
                     WFKSGLIAYVGEEWSVNLDNELRDLLTKSHIKTFEQLIRTNPQLAGHAFWYYVSKEYG
                     QTSVANLLYLVRINRSVDAGFIYVLGHDRKITGENMLNFFHERYRKDFRNAKPDSLLL
                     VKLPTRKDETLTIMAKKPFTGTLAFVTNQNGKERIYLQQADTGKRKLILKKGFRNLFQ
                     LADKEYPHIVWSADGNELICIREHRDKQLIGQYNTKTGKWTWDKIAPYYERIHSADLL
                     DKGRLVFSATVGGVSDIFVYYLSTKQTVRLTNDYYDDLDARFAQFMGRKGVIFLSNRP
                     TATNLSNRPDSIMPLQKPDLYFIEIDNPNPLIIKLTDNPQYNKANPQWIDSTHFTYLT
                     EENGNWNRKTGYLEEIVSDTVQSIYFKDGTKITVSIDSLIPQLNRQAIDTITFEPFKE
                     IKASSAWASDYTQSVVPIRYSGKSEMVFFRENQKIKAAFDKSPKKGILLPANTSYHED
                     WIKQWYISQHLTKAPGVPIPSAKPDTVSAVRVVPVKTKADTSSQKKISYFQSEFEDDE
                     TVIKFEKPTEPFIEEKSEISVESNKKFVVKNARIVPYRLAFKTDYITTRLDNSPLFGG
                     LDNYIGRKLNASGTADNFSYIPLGILLTTNFKDVLEDYVFDVGARFPTSFRGGEYFMT
                     FHDRKFRWDKAYSYYYSGQQSIIDPTLIDNNYFKNLPKALQFQLPGNFAGFPQFRTKV
                     ITNLVQAEFRYPFDLFRSIRFRTTFRNDKMHWKATEKNSLNTRNYTEQRISQRIEYVF
                     DNTLSTGINLLNGTRYKAYTEAIKGLQIQFEDPLAFNLKNGFMGIIGLDFRHYEPILK
                     KSVIAVRFAGEISFGTEKMLNILGGVDNWLVPSFNPNIPLPTGDNIAYQAIVPGLRGF
                     TYNIRNGSSYGLMSAELRIPVFRYFSEKIRSSIIRNFQVVGFFDAGMAWDGFSPFSKD
                     SPINTIYLENPPTVKVKVNYYRDPLVAGVGFGVRTSLLGYFIRADYGYGIESRIIGKP
                     VLYLAFGTDF"
     gene            complement(6489..8519)
                     /locus_tag="PLE23_05070"
     CDS             complement(6489..8519)
                     /locus_tag="PLE23_05070"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMX82671.1"
                     /translation="MSVGTITSFAQTFTGSPGLVPPDGTPALLPVDVQGLPNVMNKDF
                     GLLRLCINATHTWMEDLNIVLVAPDGQKIQVVTSVGYDQDGFANTCFDPKSSTSIGMG
                     SYPFTGSFLPMGTMQNFNNGKNPNGTWYLKVSDNYPGADDGEITSWSIEFGPDAVGPI
                     IFSGSKLPLVCFETEGKTIVSDPHVLVKMKIISDSTGALNYPNGLPNVYNGFTNIRIR
                     GQSSQSFPKKSYGIETADEVGNDTSFTILGMPSESDWVLNASFSDKTLLRNVLAHDLF
                     NKMGHYSSRKRFVEVFIDEEYQGTYILLEKIKRDKNRVNVKKLKSTDVSGKNVTGGYI
                     LKVDKGDDGGWTSTRESVKPNTHCYYQYVYPKLSDIQPEQQTYIQAFIDSLEEALYSP
                     DFRNSAGKHYTDYLDLSSFIDNFIMNELSRNIDGYRLSTYFHKEHIQDGNKLKAGPMW
                     DFDLSFRNSDFCNGALIEGWSFAEECDFDYFPPPMFMFKMMNDSIFWKELRCRWNDLR
                     DGLLTSDSLNYTIDTWVTELNGAVDRNFERWPILGNYVWPNPWPIAQNYPEEIEQLKT
                     WIAGRLLWMDENLPYKDEYCSTTSTISISQASVQLSPSPATNNLTIRFGDIRFANRPE
                     MKIINTYGQVVKNIYFDKEKQMTIDISGLVAGFYSIILNDEAKTVKSFLKIN"
     gene            complement(8699..>10443)
                     /locus_tag="PLE23_05075"
     CDS             complement(8699..>10443)
                     /locus_tag="PLE23_05075"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HMX82672.1"
                     /translation="IRLTGGPANSGCLNNGEQMGEGWSDFLALVTTVKPGDTKDKPRG
                     VGTYVTKENDNGKGFRTYQYSTDFSINPHTYGDIAGNTEVHYVGEVWNSMLWDLYWAF
                     VDKYGLDPTYKDVNSGSYKAVRLVMDALKIQPCSPGFADGRDAILAADIINYNGENGC
                     LIWDVFAKRGLGHLANQGNSNSAGDGKENFDIKPLCLNTITIKKEATPLIKAGEEIEY
                     TITVSNYKSVPATGTVVTDQIPEGATVSNANGGTVTGDVISFDLGTLASNETKIITYS
                     VSSSFKIWSQRQIFDNCENIDNWDIETGQGFNGWEPTTVESHSPSASFGISDVGQVNK
                     HSLKFLKSYNVSGDRPVMRFYHNFNTEAGNDGGYVEVSTNNGSTWTSLKDKFIRNGYV
                     SKMAYATFTLPNIEGFSGNSNGWIASYIDLSEYKGQDIIVRWKYGSNDAIGGIGWFID
                     DFEYMDLKTYNSEACATTSEGDNVCAMAPQEGTIVESQLVSANDLKQGELGIFPNPAQ
                     NYLNISLKAGTTGNYNVNILTLDGKIVKSASIGLIEGTNNQTVSVQGVPAGCYLIKIQ
                     NEKEYFVNKIIVKQ"
BASE COUNT         2840 a         2149 c         2144 g         3310 t
ORIGIN      
        1 cattttttgt aattttgtgt tgagtaaaac catgatttat atttaaacaa acaggaacaa
       61 ccagaaattt ttaactatgg caattgtaat ctcattatta aaccacaaag gtggcgtagg
      121 taaaacgact tcagccatca atataggagc cggacttgta gaacagggca agaaagtact
      181 actcgttgac cttgatccac aggctaatct tacgctttca ttaggcattc cacgccagaa
      241 aaactctatt tatgaatcaa ttagaggtga atcggagctt gatccttatc atgttaagga
      301 aaacttagat gtcatcacct catcacttga cctgtcaggt gccgaaatgg agctgatcaa
      361 tgaagccggg cgcgaatata tattgcgtga gctttttgtg cctgtagccg aggaatacga
      421 ctacatcatc attgattgtc cgccgtcact tggtttgctt acattgaatg ccctcacgag
      481 cagccagtat gttctgattc cgcttcagac cgaattcctt gctatgcagg ggcttgcaaa
      541 aattaaacaa atcattgata aagtgaagtt tagactcaac aaagagcttg agttgggtgg
      601 tgtaatagct actatgtacg attcaagaaa agtactgaac cgtgatgtag tggataccat
      661 cattaaacac tttggtgaaa aagtgtttaa aactatgatt agagataatg ttgcacttgc
      721 cgaagcgcct gctcagcgca aagatatatt tgcatacagc ccaagaagta taggagcaga
      781 agattacctt aaccttagta aagaaatcat ccaacgtacc ggtgctgaag tactggcagg
      841 tgttgaatca aaataatcct ttgaaagttt agccgtgaaa aaaacaatta gaaataaaga
      901 gcctttggca ataggtaatg atcttgaatg gacagagaca agtgaagatt tggcagtaac
      961 cactacagtc gctttaccaa agaaagccgg taaaaaattc ttcaatgatc tggatgcttt
     1021 ttttgaatct gctatagatg atttgtggaa ggaaccagta gaagaacggc tcgaaacaaa
     1081 ccaaaataaa gaagaaccag taaagcaaga tagaaaaggc aacaagaaat cattcttcag
     1141 tgggcttgat gctttgtttt caaatacagc catgccgacc actgtgtcaa ctgaagaaga
     1201 ccttagagca ttcgacgaag aaaccctaaa aagggtaaca ttcctcttta aaaaggataa
     1261 gctcgatgca cttaagaata tggctaagaa agaaaaacta tatctgcgtg atatcatctc
     1321 cagggtgctt gaccaataca tcgaagaaaa gggaatttca gccagcaaga accaaaagta
     1381 acaaatggca tttatcctcc ctgtcaaagg tatctatcct gaattcggca aaaattgttt
     1441 catagctccg aatgctacta tcgtcggcga tgtcatcatg ggcgacgact gctcggtgtg
     1501 gttcaatgct gttgtgcggg gtgatgtcaa tagcatcaaa tttggtaata aggttaatat
     1561 tcaggatggt gcagttatac attgcacata ccagaagacc atcactacat tgggtgacaa
     1621 tgtctcagta ggtcataatg ccatcgttca tggctgccaa gtgggcaata atgtattgat
     1681 aggtatgggc gctatcgtta tggatgatgc agtcattgaa gataatgtac tcatagcagc
     1741 aggagcagtc gtactcgaaa aagctcatct taaatcagga atgatatatg caggtgtacc
     1801 tgccaaaccc gtaaaggaat tgacagtcga acagtttgga cactatgtag agcgcattgc
     1861 taacaactat accatgtacg ccgactggtt taggtagcta agttttttct gtacataatg
     1921 ggctttactg ccagtctgct tcctgttcat aagcctggtc ggcagtaagt atcttcaggt
     1981 gtcttgtgcc tagatagaag gtgtcggtgt tagccattac gtgtacgatc atatttccaa
     2041 tagatatcgg tgtcccctca tctaagtcag gtactttgtt gtggctgaga tgctcaccgt
     2101 caaagacaag aaccattccc gagccaatgg tggtacagat gttgccttct ttgataatga
     2161 caccagtatc ttcgcccagg cctatgccca gcaattttgg aaatttggca acagcctccg
     2221 taagcctgcc aaaccggcct ctgttaataa aatgagaatc aactattacc tcttcaagga
     2281 agccaaagcc ttgcatcatg cttacattgc ctttaagcat agccgtagta ctcgacccgc
     2341 cacttatcat ttctttagac attgccatag cgcccgcact cgtacctgct ataataaaat
     2401 catcttcctg atagcgttgt ttaagtattt tatgtatttc agacccgcca ataatgtgca
     2461 ccagcctgcg ttggtcacca ccagataaca tgacaacatc actctttttt attaagttaa
     2521 tattgtctgc tttttttaca tcaaacttat tgcggatgtc cagaatattt acgtttttac
     2581 aaccaagttt gccaaatgct gtaagataat tcttggctac ctgttgcgga atgctcgatg
     2641 ccgttgttac gacagaaatg ttggcatctt ttccaccggc ttcttttaca atacggctta
     2701 gaatgccatc ttcaataaaa tcaagtgaat attgctccga ttgttctatg cctttgtctt
     2761 catttccgcc tactgctatc aaggttcctt ttggtggatg tggttttgcc atcttagatt
     2821 atgtcttttt ttgaatcgca aagttaaatt tttctgctca ataatagcaa cagcatgttg
     2881 gatcaaatga aattatataa taaacattgg tcagaaatct gtaccaaatg caagatacaa
     2941 tactggtttg cctatgatcc tagattcaat accatatcca taatcagccc ttataaaata
     3001 cccaagaagg gaagtgcgca caccgaaacc gactcccgcc acaagtgggt ctctgtaata
     3061 atttaccttt actttaactg tcggtggatt ttcgaggtaa atagtgttga tcggactgtc
     3121 tttgctgaat ggcgaaaaac cgtcccaagc cattcctgca tcaaagaatc caacaacctg
     3181 aaaatttctt atgatggacg accggatttt ttctgaaaaa tacctgaata caggaatcct
     3241 gagttcggca ctcattaacc cgtagctact accattgcgg atgttgtacg tgaatcctct
     3301 aaggccgggc actattgcct gataggcaat gttatcacct gtgggcaaag gaatatttgg
     3361 gttaaaacta ggtacgagcc agttgtcaac gcctccgagt atgttcagca tcttttccgt
     3421 accaaaggaa atttctccgg caaatcttac ggcaatgacg ctttttttca ggataggctc
     3481 ataatgtctg aagtcaagtc caattattcc cataaagcca ttcttaagat tgaatgccaa
     3541 agggtcttca aactgtatct gtaagccttt aatagcttcg gtatatgctt tgtaccttgt
     3601 accgttcaag agattgatgc ccgtggacaa tgtgttgtcg aatacgtatt ctattctttg
     3661 ggaaatacgt tgttcggtat aatttcttgt attgagcgaa ttcttttctg ttgctttcca
     3721 atgcatttta tcatttctga atgtggttct aaacctgata cttctgaata aatcaaacgg
     3781 atacctgaat tcggcctgaa ccagattggt aatcacctta gtccggaatt gtggaaatcc
     3841 ggcaaaattg cccggtagct gaaactgtag tgctttaggt agatttttaa aataattgtt
     3901 gtcaatgagt gttgggtcaa tgatgctttg ctgcccggaa taataatagg aatatgcttt
     3961 gtcccagcgg aattttctgt cgtggaatgt cataaaatat tcgcctcctc taaaacttgt
     4021 agggaacctg gcacctacat caaacacata gtcttcaagc acatccttaa agttggttgt
     4081 aagcaatatg cctaaaggta tgtatgaaaa attatcggcg gtaccgctgg cgttcaactt
     4141 acgcccgata taattgtcaa gtcctccaaa caatggcgaa ttgtcaagtc gggtggtgat
     4201 atagtctgtt ttgaaggcga gcctgtacgg tactatcctt gcatttttga ccacgaactt
     4261 tttattgctc tctacactta tttcagactt ttcttcaatg aatggttcag tgggcttttc
     4321 gaattttata acagtttcat catcttcaaa ctccgactgg aagtacgata tctttttttg
     4381 ggaactggtg tcagctttag tctttacagg tacaactctg acggctgaga cggtgtcagg
     4441 tttggcagaa ggtataggca ctccgggagc cttggtcaga tgctggctta tataccattg
     4501 tttgatccag tcttcgtggt aagaagtgtt ggcaggaagc aagattcctt tttttgggct
     4561 tttgtcgaag gcggctttta ttttctgatt ctcccgaaaa aacaccattt cagatttacc
     4621 gctgtacctt atgggcacta cactttgagt atagtctgat gcccatgctg aggaggcttt
     4681 tatttcttta aatggctcaa atgttatggt atctattgct tgccggttca attgcggaat
     4741 caggctgtcg attgatactg ttatctttgt tccgtctttg aaatatatcg attgtacggt
     4801 atcgcttact atttcttcca gataaccggt ttttctgttc caattaccgt tttcttctgt
     4861 caaataagta aaatgtgttg aatctatcca ttgcggattt gccttattgt actgtgggtt
     4921 gtcggtaagt ttgataataa gcggattcgg attgtcaatt tcaataaaat agagatcggg
     4981 cttctgcaat ggcataatgg aatcgggtcg attgcttaaa tttgtagcag taggtcggtt
     5041 tgatagaaat attacacctt tcctgcccat aaattgagcg aagcgtgcat caaggtcgtc
     5101 ataataatca ttggttagcc tgactgtttg cttggtggat aaataataaa cgaatatatc
     5161 cgatactcct cctactgtag ctgaaaacac aagtctgccc ttgtctaata gatcagcaga
     5221 atgtatcctt tcgtagtaag gggcaatttt atcccaggtc catttaccgg tttttgtatt
     5281 gtattggcca atgagttgct tgtctctgtg ttctcttatg caaatcagct cattgccatc
     5341 agctgaccac actatatgcg gatattcttt atctgccagc tgaaacagat ttcgaaatcc
     5401 ctttttgagt atcaatttcc tttttcctgt atctgcttgt tgtagatata ttctttcttt
     5461 tccgttctga ttggttacaa aagccagtgt tccggtgaat ggtttttttg ccattatggt
     5521 caaggtttca tcctttcttg ttggcaattt tacaagtaac agcgaatcgg gctttgcatt
     5581 ccgaaaatcc tttctgtacc tttcgtggaa gaaattgagc atattttctc cggttatttt
     5641 ccggtcatgc ccaagtacgt atataaagcc cgcatctacc gaacgattga tgcgtaccag
     5701 gtaaagcagg ttggcaactg aagtttgacc atattctttt gacacataat accaaaaagc
     5761 atgaccagca agttgcggat tggtacgaat gagctgttcg aatgtcttta tgtggctttt
     5821 ggtgagtagg tcgcgaagtt cgttgtcaag atttactgac cattcttcgc cgacataagc
     5881 tataagcccg ctcttgaacc agtcaggcag attgaacgaa acggcatttt gcactatttc
     5941 ctgcaagcta ctgccgtaga ggattgattc gatatataca cctgcaatgc cttgccgtat
     6001 aagccggtga aggtgtcggt gattaccatc aaagtagagg aatattttgt tgtcgagtat
     6061 ttttgtagat ccacccgaat attcgaatgc atcttcgagt ccgatatttg attgcttgag
     6121 gtcggtgaga tcggtataaa ctatgatttc gattttatca ctgatgctgt gttcgagcag
     6181 atcccggatg ccgtccattt ctgtttcggc tatgtgtgct gcggcaagtc ctacatttcg
     6241 gccatcgccg taccagtaca caatggttct gtcgccttca taataggtcc actctttgaa
     6301 ttttttgtga aactgaacac ggtttttacc aaactccaca cgagaagttt gtgcatcaag
     6361 cgatacgttt ccaacaatca aatacaacaa gcatgtcaga caaaataacc tcatctgttt
     6421 ataacgttca tttttgctga agtgtatgaa tatttgtaca taagttcagg tgttttgttt
     6481 gaaaatattt aattgatttt cagaaaagat tttacagtct ttgcctcgtc atttaatatg
     6541 atactataaa atcctgccac taaacctgat atgtcaatag tcatttgttt ttctttgtca
     6601 aaataaatgt tcttaaccac ttgtccgtag gtattgatga ttttcatttc aggccggttg
     6661 gcgaagcgga tatcgccgaa tcttattgtc aggttgttgg tagccggtga aggagacaac
     6721 tgcaccgagg cttgcgaaat tgatatagta ctcgtagtac tgcagtactc gtccttgtat
     6781 ggtagatttt catccatcca taacaatctg ccggctatcc aggttttcag ttgttcaatt
     6841 tcttcgggat aattttgtgc tattggccag ggattaggcc atacataatt gcctaatatc
     6901 ggccatcttt caaaattccg gtcaacagca ccgttgagtt ctgttaccca tgtgtcaatg
     6961 gtataattta agctgtcgct cgttaacaaa ccatctctga ggtcattcca ccggcacctt
     7021 agttccttcc agaatataga atcgttcatc atcttaaaca tgaacattgg tggtgggaaa
     7081 taatcaaaat cacactcttc ggcgaatgac cacccctcga taagggcacc attgcaaaaa
     7141 tctgaattgc ggaaactgag gtcaaaatcc cacatcggtc cggccttcag tttgttgccg
     7201 tcttgtatgt gttctttatg gaaataagtg ctgagcctgt aaccatcaat gtttctgctt
     7261 agctcattca tgataaaatt atctatgaac gatgacagat cgaggtaatc agtgtaatgt
     7321 ttgccggccg aatttctgaa atcagggcta tataatgctt cctcaaggct gtctatgaat
     7381 gcctgtatgt aagtctgctg ttcgggttgg atgtcactta atttcggata aacatattgg
     7441 taataacaat gtgtgttcgg ttttacactt tctctggtgc ttgtccatcc accatcatct
     7501 cctttatcca ctttgaggat atatccgccg gtgacatttt tgccacttac atcagttgat
     7561 tttaattttt tgacattcac acggtttttg tcgcgcttta ttttttcaag taaaatatac
     7621 gttccttgat attcttcatc tataaatact tcaacaaatc tttttctgct tgaataatgc
     7681 cccattttat taaataaatc atgtgccaag acattcctga gcaatgtttt gtcagaaaat
     7741 gatgcgttca acacccaatc gctttcagaa ggcatgccca atatagtgaa agaagtatcg
     7801 ttacctactt catctgctgt ttctataccg tagctttttt taggaaatga ctggctcgac
     7861 tgccctctaa ttcggatgtt ggtaaagcca ttgtacacat tgggtaaacc attcggataa
     7921 ttaagtgcac cggttgagtc acttattatt ttcattttta ccaatacatg agggtcagaa
     7981 acaatggttt tgccttcggt ctcaaaacac acaagtggta gtttgctgcc tgagaagatg
     8041 attggtccga cagcatcagg gccaaattcg atactccacg atgttatttc tccatcatcg
     8101 gctccggggt agttgtccga cactttcaaa taccatgtac cattcggatt tttaccattg
     8161 ttgaaattct gcattgtccc catcggtaag aagcttcccg taaatggata ggagcccatg
     8221 cctatcgacg tgcttgattt tggatcaaaa catgtatttg caaagccatc ctgatcatag
     8281 cctactgagg tgactacctg tatcttctga ccatcaggtg ctaccaaaac aatgttgagg
     8341 tcttccatcc aggtgtgtgt ggcgttgatg caaagcctta gtaaaccaaa gtctttgttc
     8401 attacattgg gcaagccctg tacatcaacc ggtaataatg caggcgtacc atcgggtggc
     8461 acaagccccg ggctacctgt aaatgtctgt gcaaatgaag tgattgtgcc tactgacatg
     8521 aacagtaagc aagttattgc tgaaagtaca gaacgtaaat tggatggcat aagctatgct
     8581 agtctggttt caataaaaat atcatacgaa tatagtattt tggtgaaaca ttaccaaata
     8641 aaacaaaaat ccccggatag cactacccgg ggattttgtg ttcaaatgag ctggtatgtt
     8701 attgttttac aatgattttg tttacaaaat attccttctc attctgtatt ttgataagat
     8761 aacatcctgc aggtacgccc tgaacactga ctgtttggtt attcgttcct tctatcaggc
     8821 ctatacttgc cgattttacg atttttccgt cgagcgtcag tatgtttaca ttataattgc
     8881 ctgtggtgcc agctttaagt gagatgttaa gataattttg ggctgggtta gggaagatgc
     8941 ccaattcgcc ttgtttgaga tcgttggcag atactaattg agattcaacg attgttcctt
     9001 cttgtggagc cattgcacag acattgtctc cttcagatgt agtggcacaa gcttcgctgt
     9061 tgtatgtttt caagtccata tattcgaagt cgtcaataaa ccatccaata ccgcctattg
     9121 catcattaga gccgtatttc catctcacga tgatatcctg gcctttatat tcagataagt
     9181 cgatataact ggcgatccag ccatttgagt ttcctgagaa accttctatg ttaggcagtg
     9241 tgaaggtggc atacgccatt ttgctcacat atccgtttct gataaatttg tctttcaggc
     9301 ttgtccatgt gctgccatta ttggtggaaa cttcgacata tccaccgtcg ttgcctgctt
     9361 ctgtgttgaa attatgataa aatcgcataa caggccggtc gccgctcacg ttatatgatt
     9421 tcaagaactt caggctgtgc ttgttgacct ggcctacgtc agaaataccg aagcttgcgg
     9481 atggtgaatg gctttcaaca gtggttggtt cccacccatt gaatccctgt cctgtttcga
     9541 tatcccaatt atcaatgttt tcacagttgt caaaaatctg tctttgtgac caaattttga
     9601 aagacgaact cactgagtat gtgatgattt tggtctcatt ggatgcgagt gtaccgagat
     9661 caaaagaaat gacatcaccg gtgactgtac caccgttggc attacttact gtagcacctt
     9721 caggtatttg gtcagtgaca accgttccgg tggcgggtac tgatttatag ttgcttactg
     9781 ttatggtgta ttctatttcc tcacctgcct ttatcagcgg agttgcttcc tttttgatag
     9841 tgatggtgtt caggcaaagt ggttttatgt cgaaattttc tttaccgtca cctgcggagt
     9901 tagaatttcc ctgatttgcc aaatgtccaa gacctctttt tgcaaacacg tcccagatga
     9961 ggcatccgtt ttcgccattg tagtttatga tatcagctgc aagaatagca tctcggccat
    10021 ctgcaaagcc aggactacaa ggctgtatct tcagggcatc catcaccaga cgtaccgctt
    10081 tgtacgaacc tgagttaaca tccttgtagg tagggtcgag accatattta tctacaaatg
    10141 cccaatatag gtcccacagc atgctgttcc atacttcacc tacataatgc acttcggtat
    10201 tacctgcgat atctccatag gtatgtgggt tgatactgaa atcagtagag tactgataag
    10261 tacggaatcc tttaccattg tcattctctt tggtgacata agtgccaaca cctcttggtt
    10321 tatctttggt atcgccaggt ttgacggtag tgacaagagc caaaaaatcg ctccaacctt
    10381 cgcccatttg ttcgccattg ttcaaacaac cgctgttggc gggaccgcct gttagtctta
    10441 ttg
//
LOCUS       DAOQQY010000069        10389 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2341_length_10389_cov_5.702342, whole genome shotgun sequence.
ACCESSION   DAOQQY010000069 DAOQQY010000000
VERSION     DAOQQY010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10389
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2341_length_10389_cov_5.702342"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="PLE23_05080"
     CDS             <1..376
                     /locus_tag="PLE23_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX82673.1"
                     /translation="DRVKNTKNITIHWNSETEEILGGDDVTGVRIRNNKTGELTEIPV
                     KGFFVAIGHKPNTEIFKDWLEMDETGYLIPVAGTSRTKVPGVFVSGDAQDKVYRQAVT
                     AAGSGCMAALDAERYLVSIGIH"
     gene            408..1475
                     /gene="fbaA"
                     /locus_tag="PLE23_05085"
     CDS             408..1475
                     /gene="fbaA"
                     /locus_tag="PLE23_05085"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HMX82674.1"
                     /translation="MAKFSAGVVWGNEAKDLLQYANENDFALPAVNVTGTNTVNAVLE
                     TAREVNSPVMIQYSNGGAAFFAGKGLSNVGESAAILGGISGAMHVHTMAKAYGVPVIL
                     HTDHCAKKLLPWVEGLLHAGEKFFEERKRPLYSSHMLDLSEEPIEENIAISKSFLERM
                     DKLGMFLEIELGVTGGEEDGVDNTDVDNSKLYTQPSEVSYAYEELSRISRNFTVAAAF
                     GNVHGVYKPGNVKLEPKILKNSQEYIKNKFNTGDNPVHFVFHGGSGSSREEIREAISY
                     GVIKMNIDTDMQWALWSGVKDFYEAKRDYLQGQIGNPEGDDKPNKKYYDPRVWLREGE
                     KAFVNRLKESFEDLNCINRNQ"
     gene            complement(1500..3428)
                     /gene="dnaG"
                     /locus_tag="PLE23_05090"
     CDS             complement(1500..3428)
                     /gene="dnaG"
                     /locus_tag="PLE23_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMX82675.1"
                     /translation="MISQKSIDEIIDTARIEEVVGDFVTLKRRGINFIGLCPFHNEKT
                     PSFNVNPARNIFKCFGCGQGGDSVQFIMEHDGMSFTEALRYLAAKYKIEIEETKPSAE
                     QQILIDETESLHIINQYAVGFYSDQLFNTDMGKSVGLSYFKQRGFLEETIKKFNLGYS
                     PGERDAFTRNAVKAGYNPDLLKKLGLTTASDNDFFRSRVIFPIHGLSGKISAFAGRTL
                     SSEAFGPKYLNSPESAVYNKSKTLYGLFHAKQAIRKHDECLLVEGYTDVISLHQGGVE
                     NVVASSGTSLTVEQIRLIKRYTQNILIVYDGDPAGIKAALRGIELILEEDLKVKIVLL
                     PDKHDPDSFFKEVGHSGFTEFIENNKKDFVSFRISLAADEIKGDPVKKMGFIQEIVRT
                     LAKIPHQLVRAEYIKEISEKYKIAEQLITLDINRQVIKMLREREEKAEKDKKQQEISI
                     DDSEQPETATEIRVSTYDHSVQEKDLIRVLIHFGDKIFDENSGATVAQYIYNNTVDIL
                     QYFDVPLYKLILENVANLIDSGQSPGPDYFLKHQDSEIRNVAMNALQEQYVYSEKWNK
                     PLQTQKPPEENFVKDALFVVRILKYRKFARICKEMEQKMLESKSPEDEIKYLKMNMKL
                     QELKRETGKPLNIIIFGE"
     gene            complement(3516..4235)
                     /gene="dapB"
                     /locus_tag="PLE23_05095"
     CDS             complement(3516..4235)
                     /gene="dapB"
                     /locus_tag="PLE23_05095"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMX82676.1"
                     /translation="MKISLLGYGKMGKTIEKIALQHGHEIVFKINRENLDDLLKINHT
                     NTDVAIEFSMPEAAVSNLKHCLLNGVPVVCGTTGWLERWDEISQLCKDNDGAFLYASN
                     FSLGVNIFFSLNKYLSKMMSGFPQYKVSIEEIHHTQKLDAPSGTAISLANDIIQTHSV
                     YTAWEKDTATNDDKIAITSIRTDPAPGTHTIRWNHPIDTIEIRHTAHSREGFALGAVL
                     AAEWIIGKKGTLTMQEMLGFV"
     gene            complement(4394..6415)
                     /locus_tag="PLE23_05100"
     CDS             complement(4394..6415)
                     /locus_tag="PLE23_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX82677.1"
                     /translation="MKKSFFTVLSSLLLTHIALLPLFAQSDSTILGKDLDQVVITAQY
                     APTDIRQTVNSVKILTSRTIEQRLVLSLYELLQTEPNIRLSQDPILGSVISINALKGE
                     NLKILIDGVPVVGRLNGNIDAGQIPLNAIQKVEIIEGAQSLLYGSDASGGVINIITKK
                     SQIAKFEGNISSQYENNGFRQLAAGTGYSSGKWIAQANANYLQFVPATDTSMGRDQLW
                     NPKQQLSGKGIIRFLPNERTDLRVNVGFMKEQVDNPGDLKRPQFKPYAFDDYYFTNRK
                     EIGFHGEKWTKHNRLIQATASWNTFSRIKNSYRHDFQSNTDELLTGLQDTASSTAVLG
                     RLTVAHDVRNTNFGYIFGIENYYEQGTGTRLADTTLSETGMASTNDLGIFGSGKYKIL
                     SNLTAQTGARLTINNKYGTAITPSTWVHWQPKPALHIRFSWAYGFRSPAIKELYFSFI
                     DVNHYVTGNPQLKPEKSLNLRSEISFDLIRNRNSELTATISGFYNRMSDRIVLTALGP
                     VHYEYRNVDNFTTLGGTAGITYKYRNVFNFRSDIVNTGFNNINDEVDVSKGKILWSPE
                     WVNDLTLTFFEGKLDLNIWHKWTGKTPFFYNDNGKTTEGFTESWSMLNSGISGRLWKN
                     KLRINLGVKNIFNIRQLQVNNINGIHVEVSNQQALHWGRSLYAGMSVKF"
     gene            complement(6434..7726)
                     /locus_tag="PLE23_05105"
     CDS             complement(6434..7726)
                     /locus_tag="PLE23_05105"
                     /inference="COORDINATES: protein motif:HMM:NF025430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82678.1"
                     /translation="MIRQFTLTLSILIISVISLSAQEFQEISCGAGYKYQSYVQLAAG
                     TEKQVNNDAWDIAFTAFGFQDAGILINESSASIQGENVPQTELYYTGNSDFNASFDLD
                     QIIKGAKLYNSKASWNDGAFNALKDTTNAFDYGWGAYNPATHSVTGTKVFVLKLRSGK
                     YIKLMVEALNGTDYVIRYADIDGQNEKTKTFSKTKDNAGQKFIYYTFTDDKIVDILPQ
                     GGFDLFWGRYVSWATDPNGTIQQWYTVTGILTAPGVQTAVAKGVNPETVSVDPYLNSF
                     SQKIDQIGYDWKTLVGTSWTLATDRAFFVKLPDNNVWKLAIVDFEGSATGTATLQKYS
                     LGNPTSTHDLKGTKLSFYPNPIQNELIISLDVAQLYDYQYDVVVTDLAGRPVMSTKVN
                     ATEGWNVLKLDTSNLASGAYLVRVSNSNGTLAEKIIKY"
     gene            8034..8600
                     /locus_tag="PLE23_05110"
     CDS             8034..8600
                     /locus_tag="PLE23_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HMX82679.1"
                     /translation="MAEKQNRQRQARILRDFRKIHRWTGALLFVFFFIISVTGLLLGW
                     KKHSNGILLAKTHKGTTTNLANWLPLDSLHKRACYHLHKEVSPGLSLKLNRIDVRKDK
                     GTVKFVFDEHYWGIQLDGATGGLLHVEQRRADFLEQIHDGSILDHYLGTSNGQIKLVY
                     TTIMGLALLIFTVTGFWLWYGPKRMRKT"
     gene            8792..9349
                     /locus_tag="PLE23_05115"
     CDS             8792..9349
                     /locus_tag="PLE23_05115"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HMX82680.1"
                     /translation="MMKQSQFLKIAVLALMVIFAACKKDETNNPTPSGGPFVAKVDGT
                     AFPTTDAKLTKAKYLSATKTLQIIGQPSDQKEGIILTLLNFDSGFTSWKPGTYSFDPT
                     NITAGKYLASAEYNKWNGSGYDQWFNNWDNDKTGQIIIESITDTHVKGTFLFNAEKKL
                     PNGSSDPTNVKKITEGAFDLDITKL"
     gene            complement(9468..>10389)
                     /locus_tag="PLE23_05120"
     CDS             complement(9468..>10389)
                     /locus_tag="PLE23_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMX82681.1"
                     /translation="TSCSTEAANMIFEGAVRNLGVKRIISSPIEHHCVLHPLEKHSRN
                     GIEVVYLNVHKNGSIDYEQLETLLSSDDAKALVALMHANNEIGTLNDLDKISSLCREN
                     KAYFFSDTTQTIGHLPIDVEKTKFDFIIGSAHKFYGPKGAGFVYMNADVNIEPLLLGG
                     SQERNMRSGTENIIGIAGMAAALEYTLANMPSFQEKIKNLREYLQFRILSDIPGTYIN
                     GYAEHFLPKILSVSFPPSPRNEMLLMNLDINGIAASGGSACSSGVETKSGVLDYIGEP
                     HDRKTVRFSFSHHNTIQELDKVIEVLLKLF"
BASE COUNT         2940 a         2004 c         2236 g         3209 t
ORIGIN      
        1 ggaccgagtt aaaaacacaa aaaatattac tattcactgg aacagtgaaa cagaagaaat
       61 attaggtgga gacgatgtaa ccggcgtccg tatcaggaat aataaaacag gagaattgac
      121 agaaataccg gtgaaagggt ttttcgtggc tatcggtcac aaacctaata ccgaaatttt
      181 caaagattgg ctcgaaatgg acgaaacagg ctacttgatt ccggtagcag gtacttccag
      241 aaccaaagtg ccgggtgtgt tcgtaagtgg tgacgcccag gataaggtgt acagacaggc
      301 tgttacagct gcaggctcgg gttgtatggc agcacttgat gcagagagat atctcgtgtc
      361 aataggaata cattaatatt gaacaatata accaaaaact tgaaagtatg gctaaattta
      421 gtgctggtgt agtatggggc aatgaagcca aagatttgct acaatacgcc aatgaaaacg
      481 acttcgcact accggcagta aatgtcaccg gaaccaacac ggtaaatgct gtgctggaga
      541 cagctcgtga agtaaattcg ccggtgatga ttcaatactc taatggaggt gcagctttct
      601 ttgcaggaaa aggcttatct aatgtaggtg aaagtgcagc tatccttggt ggaatatcag
      661 gtgctatgca tgtacataca atggcaaagg cctatggtgt gccggtcatc ttgcataccg
      721 accactgtgc aaagaaacta ttgccttggg tagaaggttt gctacatgca ggtgaaaagt
      781 ttttcgaaga aagaaaaaga ccgctttaca gctcacacat gctcgatctt tctgaagaac
      841 ctattgagga aaatatagca ataagcaaga gtttccttga acgaatggat aaacttggta
      901 tgtttctcga gattgaactc ggggtaaccg gtggcgagga agatggtgtt gacaatacgg
      961 atgtcgataa ttctaaatta tatactcaac catccgaagt gagctatgct tatgaagaat
     1021 tgtctcgtat cagcaggaat tttacagtag ctgcagcatt tggaaatgtg cacggtgttt
     1081 acaagcccgg aaatgtaaaa ctggagccga aaattttgaa aaattcgcaa gaatacatta
     1141 aaaacaaatt taatacaggc gataatcctg tacatttcgt gtttcacggc ggtagcgggt
     1201 cgagtaggga agagatacgc gaagctatat cttatggtgt catcaagatg aatatcgata
     1261 ccgatatgca atgggctttg tggtcaggcg taaaagattt ttatgaagca aaacgtgact
     1321 atcttcaagg tcaaatcggg aacccggaag gtgatgacaa acctaacaaa aaatattacg
     1381 atccgcgtgt ttggctcaga gaaggagaga aggctttcgt caatcgactg aaagaatcgt
     1441 ttgaagatct taactgtatt aatagaaatc aataaaccaa ttgaagcaga agcgggaaat
     1501 cactcaccaa atataatgat gttgagtggt tttcctgttt cacgtttgag ctcctgaagt
     1561 ttcatattca ttttcaggta ttttatttcg tcttccgggc ttttagactc gagcatcttt
     1621 tgttccattt ccttacagat tcttgcgaat tttctgtatt ttagtatcct taccacgaag
     1681 agtgcgtctt ttacgaaatt ttcttcaggt ggcttctgtg tctgcagagg tttgttccat
     1741 ttttcactat acacatattg ttcctgtagg gcgttcatag caacattgcg tatttcgcta
     1801 tcctgatgtt tgagaaaata atcaggtccg ggtgattggc ctgaatcgat gagattggct
     1861 acattttcga ggataagttt atacaaggga acatcaaaat attgcagaat atccactgta
     1921 ttgttataga tgtattgggc tacagtagct cccgaatttt catcaaaaat tttgtcacca
     1981 aaatggatga gtaccctgat gaggtctttt tcctgcacag aatggtcata ggtcgatacc
     2041 ctgatttcgg tagcggtctc tggttgctca gagtcgtcga ttgatatttc ttgttgtttt
     2101 ttatcttttt cagctttttc ctctctttcc cttaacattt ttatcacctg cctgttgatg
     2161 tcaagcgtaa tgagttgttc ggctattttg tatttttcag agatttcctt aatgtattct
     2221 gcccttacca actggtgagg aatcttagca agcgtgcgta caatctcctg gatgaagccc
     2281 atttttttta ccggatcacc ctttatttcg tctgctgcta agctaatgcg gaatgaaacg
     2341 aaatcctttt tattgttttc gataaattcg gtaaagccac tgtggcctac ctccttaaag
     2401 aatgaatccg gatcgtgttt gtcgggcaat aagacgatct tgacctttaa atcttcttca
     2461 aggatgagtt ctatgccgcg taatgcagct ttaatacctg ccgggtcacc gtcataaact
     2521 atgagaatat tttgggtata tctttttatc agccttatct gctctaccgt tagagatgtg
     2581 cccgatgagg caacaacatt ttctacaccg ccttgatgca atgagataac atcagtataa
     2641 ccttctacca ataggcattc gtcgtgtttg cggatagctt gtttggcatg gaataaacca
     2701 tacagagtct tggacttgtt ataaactgct gactcaggtg agttgaggta tttggggccg
     2761 aatgcttcac tggaaagtgt tcgtcctgca aaagcagaaa tttttcctga cagcccgtgg
     2821 attgggaaaa taacacgact cctgaaaaaa tcattatcag aagcagttgt gagaccgagt
     2881 tttttaagca aatcagggtt gtagcctgct tttacagcat ttctggtgaa tgcatctctt
     2941 tcgcccggcg aatatcctag gttaaatttt ttaatggttt cttctaaaaa tcctctctgt
     3001 ttgaaatatg aaaggcctac actcttgccc atgtcggtat tgaaaagctg gtcggagtag
     3061 aaccctaccg catattgatt gatgatgtgg agactttcgg tttcatcaat gaggatttgt
     3121 tgttctgcag atggttttgt ttcttcaatc tcgattttat atttcgctgc cagatagcgt
     3181 aaggcttccg taaaactcat gccatcgtgc tccatgatga attgtacaga atcgccacct
     3241 tggccacaac cgaagcattt aaagatattc cttgccggat tgacattgaa tgaaggtgtc
     3301 ttttcattgt gaaacggaca caaaccaata aaattaatgc cccttcgctt caatgttaca
     3361 aaatcaccca ccacctcttc tatgcgtgcg gtatcaatta tttcgtcaat ggacttctgt
     3421 gaaatcattt gggtgagttg aggacatgaa gatactgaat tctatgcaat cgtgtcaatg
     3481 ttgtggtctt tttactttgc aaaatgctga ttttttcata caaaacctag catttcctgc
     3541 atagtaagtg tgcctttttt cccgatgatc cattctgcgg caagcacagc acccagcgca
     3601 aaaccctcac gcgaatgagc tgtatggcgt atttcgatgg tatcaatcgg atgattccac
     3661 cttatggtat gtgtgcccgg tgccgggtca gtacggatgg aagtaatggc tattttatca
     3721 tcattggtag ctgtatcttt ttcccaggct gtataaacag aatgcgtctg gatgatatcg
     3781 ttggctaagc taatagctgt gccactgggg gcatccaatt tttgtgtgtg gtggatttct
     3841 tctattgaga ctttgtattg aggaaatccc gacatcattt ttgatagata cttattaagg
     3901 ctgaagaaaa tattgacacc caggctgaaa tttgaagcat ataaaaatgc gccatcatta
     3961 tccttacaga gttgacttat ttcatcccaa cgttcgagcc atccggtagt gccacacacg
     4021 accggaactc cattcagaag gcagtgtttg agattgctaa cagctgcttc cggcatggag
     4081 aattctatgg caacatcggt gttggtgtgg tttattttaa gcagatcatc cagattttct
     4141 ctgttgattt tgaagacaat ttcatggccg tgttgtaggg caattttttc gattgttttt
     4201 cccattttac cataacctaa cagacttatt ttcataataa aatgtgtaaa ttagaacaag
     4261 aaaacttaaa actctgtcaa tagaattgaa caaaattaac gaaagccatt cagtgtgagt
     4321 gatatttggc aaaaaaatac cgaaaaaatg taataaatat ttagagtctg aacgttatca
     4381 cttacattgg atatcagaat ttgacagaca taccggcata caaacttcga ccccagtgca
     4441 aagcctgttg gttgcttact tcaacatgga taccgttgat attgttgacc tgtagttgtc
     4501 tgatgttgaa gatgtttttt acgccaagat tgatgcgaag cttatttttc cacaatctac
     4561 ctgaaatacc cgaattgagc attgaccaac tttctgtaaa tccttcggtg gttttgccat
     4621 tgtcgttgta gaagaatgga gtttttcctg tccatttgtg ccagatattc aagtctaatt
     4681 ttccttcaaa gaaggtaagt gtaagatcat tcacccattc aggcgaccac aggattttgc
     4741 ctttcgatac atccacctcg tcatttatat tattaaagcc ggtgttgaca atatctgatc
     4801 gaaaattaaa gacatttcga tatttgtaag taataccagc tgtgccacca agcgttgtaa
     4861 aattatccac attacggtat tcataatgta ccggaccaag ggcagtaaga acaatgcggt
     4921 ctgacatccg gttatagaat ccgctgatag tggcagtaag ttcgctgttg cggtttctga
     4981 tcaggtcaaa actgatttct gaacgaaggt taagagattt ttcgggtttg agctgcggat
     5041 tgccggtgac atagtggttt acatctataa atgagaagta aagttcctta atagccggtg
     5101 agcggaaacc ataagcccat gaaaatctga tgtgcaaagc gggctttggc tgccagtgaa
     5161 cccaagtaga aggtgtgatg gctgtaccat atttgttgtt gatggtaagg cgagcacccg
     5221 tctgagcagt aagattagaa agtatcttgt atttgccact gccgaatatt ccaagatcat
     5281 tggtacttgc catacctgtt tcactcagtg tagtatctgc caggcgagta cctgtacctt
     5341 gttcgtagta attttcaatt ccgaaaatat atccgaaatt cgtattcctt acatcatgtg
     5401 ctactgttaa tcttcccaat acagctgtgg agctcgcagt gtcctgtaat cctgttagta
     5461 gttcatctgt attgctttgg aaatcatgtc tgtacgaatt ctttatccga ctgaaagtat
     5521 tccagctggc tgtagcctgt atgagcctgt tgtgttttgt ccatttttca ccatgaaaac
     5581 caatttcttt tctattggta aaatagtaat cgtcaaaggc ataaggctta aactgtggtc
     5641 gtttcagatc gcccggatta tctacttgct ccttcatgaa ccctacattt actctcaggt
     5701 cggtacgttc gtttgggagg aatcggatta ttcctttgcc cgataattgc tgtttggggt
     5761 tccaaagctg atctctgccc atgcttgtat ctgtagccgg tacgaattgg aggtaattgg
     5821 cgttagcctg tgcaatccat ttgcctgaag aatagcctgt acctgccgcc aattgtctga
     5881 atccgttgtt ttcgtattgg ctgctgatat ttccttcaaa ttttgctatt tggcttttct
     5941 ttgtaatgat attgataacg ccacctgatg catctgaccc atagagcaag gattgagctc
     6001 cttctatgat ttcgactttc tgaatggcat tgagtggtat ttgtcctgca tcaatatttc
     6061 cattcaggcg cccaactacg ggtacaccgt caatcagaat tttcagattt tcacctttga
     6121 gtgcgttgat gctgatgaca ctacccaata tcgggtcttg tgacaacctg atattgggct
     6181 ctgtctgtaa taattcatac aagctgagta ccaaacgttg ttctatagtt ctgctagtaa
     6241 gtatttttac tgaattaaca gtctgcctta tatccgtagg tgcatattga gcagtaatga
     6301 ctacctggtc aagatctttt cccaagatgg tagagtcgga ctgcgcaaaa agcggtaaca
     6361 gagcaatatg tgtcagcaga agactgctta gaacagtaaa aaacgatttt ttcaaacctg
     6421 taggcgtatg tgattaatat ttgatgattt tttctgccaa tgtgccgttg ctgttgctta
     6481 cacgtactag atatgcaccc gaagcaaggt tggaggtatc cagcttaagt acattccagc
     6541 cttctgttgc atttactttt gtactcatta caggtctgcc tgccaggtca gtgactacaa
     6601 catcatattg ataatcatat aactgtgcta catcaagaga aataatcagt tcgttttgta
     6661 tcgggttagg gtagaagctt agctttgtgc ctttcagatc atgagttgaa gttggattgc
     6721 caagggaata tttttgaagt gtggctgtcc cggtggcgct accttcgaaa tcaactatgg
     6781 ccagcttcca aacattgttg tcgggcaatt tgacgaaaaa tgcacgatcc gtagcaagtg
     6841 tccaacttgt acccacgagc gttttccagt cataaccaat ctggtcaatc ttttgcgaga
     6901 aactgttcag atatgggtca acgcttactg tctcaggatt gacacctttg gcaactgctg
     6961 tctgtacacc aggtgcagtc aatatgcctg tgacggtgta ccattgctga atggtgccgt
     7021 tagggtctgt tgcccatgac acataccggc cccagaaaag atcaaagcct ccttgtggca
     7081 aaatgtccac aattttatca tccgtaaatg tgtagtaaat gaacttttgg cctgcgttat
     7141 ccttggtttt gctgaaggtt ttggtttttt cattttgacc gtcaatatct gcatatctga
     7201 taacataatc agtaccattc aacgcttcga ccatcagttt aatgtattta ccgcttctga
     7261 gttttagtac gaatactttt gttccggtaa cggaatgtgt ggcaggattg tatgcgcccc
     7321 aaccatagtc gaacgcattc gtagtgtctt tgagtgcatt gaaggctcca tcattccatg
     7381 aggctttact gttgtacaat tttgcacctt ttataatctg gtcaaggtca aatgaggcat
     7441 tgaaatctga gtttccggtg tagtagagtt ctgtttgagg aacgttttca ccctgaatgc
     7501 tggcggaaga ttcgttgata aggataccag catcctgaaa accgaaagca gtaaatgcaa
     7561 tatcccaggc atcattgttc acctgttttt ctgtacctgc tgctaattgt acatagcttt
     7621 ggtatttata tccagcgccg cagcttattt cctgaaattc ttgtgcagaa aggctgataa
     7681 cagaaataat gagaatggac agtgtaagtg taaattgtct gatcatcttg aatttgtttt
     7741 attttttaat aaaatatagc gcaaagatat ttggaagcaa agcaggcgac acatttttat
     7801 ctgtcaaact tgtgtcataa atgtattgcc gtatgtattg gccggaattt ttatcaaatc
     7861 aattttctca gactaagatg ctttatctgg gtttaagaaa atttcaactg aatgaaaaac
     7921 aattttcctg attcaaaggt taattagctc taaaggcagg agttaatatt tgaatggaac
     7981 cctgttagag atgcatttta agataacaaa atcaggcata aagcgataaa agaatggcag
     8041 aaaagcaaaa ccgacaaaga caagcaagaa tactacgaga cttccgcaaa atacatcgct
     8101 ggacaggtgc attgctattt gtatttttct tcattatttc tgttacaggt ctgcttcttg
     8161 gctggaaaaa gcatagcaac ggcatattac ttgccaaaac acacaagggc accactacta
     8221 atctggcaaa ttggctcccg cttgacagtc tccataagcg agcatgttat catctgcata
     8281 aggaagtgtc accgggtctt tccttaaaac ttaacagaat cgatgtgcgc aaggacaaag
     8341 gcactgttaa atttgtgttt gatgagcatt attggggaat acagcttgat ggtgcaacag
     8401 gtggcttgct gcatgtggaa cagagacgag cagattttct ggaacagata cacgatggtt
     8461 ctatactaga tcattacctg ggtacatcta atggccaaat taagcttgtt tacactacaa
     8521 taatggggtt ggcgttgctt atttttacgg ttaccggctt ctggttgtgg tatgggccaa
     8581 aaagaatgcg taaaacttga cagatttagt caatttaaaa aaaataaaca aaaaattata
     8641 tatacataaa ataatgtaaa tcaatatttt aagtaaaatg atacgtcatg tatttaaaag
     8701 ccgataaagc atattctatc aaaatgggca gttcggccca tttgataaat acttgagatg
     8761 aggcaatttt gggaaaaatt aatctaaaac aatgatgaaa caatcccaat ttttgaaaat
     8821 tgccgtactg gcactgatgg tcatttttgc agcttgcaaa aaagatgaaa cgaataaccc
     8881 gacaccatcc ggtggtcctt ttgtagcaaa agtagatggt acggcatttc ccacaacaga
     8941 cgctaagtta acgaaggcaa aatacctttc tgctacaaag actcttcaaa tcattggaca
     9001 accgtctgac cagaaagaag gtataatatt aaccctgtta aacttcgaca gtgggtttac
     9061 ttcctggaaa ccgggtacat acagctttga cccaacgaac attactgcag gcaagtatct
     9121 ggcaagtgca gaatacaata aatggaacgg cagcgggtac gaccagtggt tcaataattg
     9181 ggacaatgac aagaccgggc agataatcat cgagtccata actgacacac atgttaaagg
     9241 gacattttta tttaatgcag aaaagaagct ccctaatgga agttccgacc ctacaaatgt
     9301 aaagaagatt acagaaggtg catttgacct ggatatcacc aagctgtagg tacttttttt
     9361 acttttgcat actttcatat tacaaacaac agaaggctgc catcaactgg tggccttatt
     9421 attttatgga taaaagtcgt atagattgta cttaaaatat attttcttta aaaaagtttc
     9481 aacagtactt cgatgacttt gtccaattct tgtatggtgt tatgatgtga aaatgagaat
     9541 cttaccgtct ttcgatcgtg cggttctcca atgtagtcaa gcacacctga cttggtctct
     9601 acgcccgagc tacaggcact gccacctgat gctgctatcc cgttgatatc gaggttcatc
     9661 agcaacattt cgttgcgtgg tgagggtgga aaagagacgc tcaatatttt cggtaaaaag
     9721 tgctctgcat aaccatttat ataggttccg ggaatgtcgg aaaggattct gaactgaagg
     9781 tattcacgga gatttttaat tttctcttgg aatgatggca tattggcaag cgtgtactca
     9841 agagcagcag ccataccggc aataccgatg atattttctg tacccgaacg catatttcgc
     9901 tcctgcgacc cacccagtag aagcggctcg atattgacat ctgcattcat atatacgaag
     9961 ccggcgcctt tggggccata aaatttatga gctgaaccaa tgatgaagtc aaattttgtt
    10021 ttttctacat cgatgggtag atggccgatg gtctgtgtcg tatcggaaaa aaagtatgcc
    10081 ttattttctc tgcaaaggct tgatattttg tccagatcgt tgagtgtgcc tatttcattg
    10141 ttggcatgca tgagcgcaac aagggctttg gcatcatctg aagataggag cgtttcgagc
    10201 tgttcgtagt cgatgcttcc gtttttatgg acgttcagat agactacttc tatgccattt
    10261 ctgctatgct tttcaagtgg gtgtaataca caatggtgtt cgatgggtga tgaaatgatc
    10321 cttttgacac ccaggttgcg gacagcgcct tcaaaaatca tattggctgc ttcggtactg
    10381 catgaggtg
//
LOCUS       DAOQQY010000070        10277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2372_length_10277_cov_6.905987, whole genome shotgun sequence.
ACCESSION   DAOQQY010000070 DAOQQY010000000
VERSION     DAOQQY010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2372_length_10277_cov_6.905987"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1502
                     /locus_tag="PLE23_05125"
     CDS             <1..1502
                     /locus_tag="PLE23_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX82682.1"
                     /translation="FGDINTSLLLGSQINKRTFNSVFARGQFLTIPNFFNLSNASDLY
                     ADQSSSVVKLAGVFASLDLDYKNWIYVNLSGRNDWASTFGPKAKNSFFYPSASIAFVP
                     TEIMAQNDFLNYFKVRASFAQAGREPSAYSSATYYTKPTFTDGFTDGISFPYLGNNGF
                     GISGTLGNAELRPEINTTFEVGADFKLLKNRINFGITYYNSKATDLLIARPIASSTGF
                     GAFVANSGEMTNKGIELELGIDIVKTKTFNWNIEGNFSKNTNNVDKLVEGVDQFSIEA
                     AFTGVGSYAIVNQPYGAIFGTKWKRNDAGQLIIGANGLPLKEAEEGFLGNPYPDWISA
                     IRNTFTFKGFSLTGLLDIRQGGDIWGGTIGRLNNIGRTAASADRTHTYVIPGVKADGT
                     PNDIAISPNFYYARYLGDAGGATESFIFDGSWVRLREVTLSYSLPKFKKLIQNASVYV
                     TGRNLWLKTKYPGVDPESSLTGAGSNIGGFDYFTMPGSKSIIVGLNIGF"
     gene            1532..2887
                     /locus_tag="PLE23_05130"
     CDS             1532..2887
                     /locus_tag="PLE23_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HMX82683.1"
                     /translation="MKNYIFLALLLTGLVFTGCKKYVEGEDISPNSPSNVTEGLLLSG
                     LEVATFSNFGGDLSRVTSVLTQQSTGNLFQYEDLAKYDITESTVSNIWNQLYSGSLIN
                     AQLLIDKAGDKNRHYRGIGRVLKALNLGLATDLWGDVPNKEALGGLNGNFNAAFDSQE
                     SVIADIQQTLDGAIADLQSTENVIAPGADDYIFGGDASKWLMNAYLLKARYANRLSKK
                     DATGSANKALEALNGAYAAGYTGNGSNLYAIFGPGVTESNQWYVFNQDRAGYVSMGST
                     LVGMMSAINDPRLAFYAAKDGSGNYSNESSIGAKYGSAESKLPLLTYAEAKFIEAEAQ
                     MRLGNKAAAAAAHNAAVIANLNDVTGGGDAAFEAANANEDAASITMSKIMSQKYIAMF
                     TQPEVYSDWRRTNIPALTPNAGANLTEIPRRFPTAQDERLYNTKAVVNSNLLDPVWWD
                     K"
     gene            3106..6345
                     /locus_tag="PLE23_05135"
     CDS             3106..6345
                     /locus_tag="PLE23_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HMX82684.1"
                     /translation="MKKLTLLISMLLIASASLLAQRTVVGKVTDSAGSPLPGATVLVK
                     GTSEGTAADDNGNFRLNVPANANTLVVSYTGFNTQEVLLGASNVVNVALQEGVTLSET
                     VVTALGITKKKNELPYSAQTVGGDDVSKSRDGNVVNALSGKVAGLNIKRNNSLGGSTN
                     IVLRGTKSLTGNNQALFVVDGVPVDNSNTNTSDQLTGRQGYDFGNAAADINSDDIDNI
                     TVLKGAAASALYGSRAANGVIVITTKKGSKAKGLGVTLNSGLNVGTIEKSTFATYQKK
                     YGAGYGAYYEDPSGFFLYRDIDGDGTDDLVTPLTEDASWGAKFDPNLKVFQWDAFDPT
                     SPNYKKATPWVAAANDPSTFFETAIGTNNGVMIDGSNDKGYFKLGYNHITDKGILPNS
                     NINKDMINFGGSYNLTRKLTAYSAINYTGISAKGRYGTGYGDRNLMTNFRQWWQTNVD
                     ISELRQAYDRTKQNVTWNWADPTILAPIYWDNPYWTRYENYETDGRGRYFGNVGLEYK
                     LLSWLNLKGQMSLDRYSEYQEERIAVGSVDVPKYSRYDRTFQEINYDLLLSSTPLRLS
                     ESLHFNFLLGSNVRKNEIYAIADGTNGGLAIPGIYALSNSVSARNAPDESLTKLQVNG
                     LFAQTSFNWDNSVFLDLSVRRDKSSALPKDNNVYIYPAASLGLLFSNWTGTGSVFSFG
                     KLRFNYAQVGNTPPPLVVDDVYNIGVATLLDRGVYATSFGSAPLASVSSTKNNPGLKP
                     ESTKSFEVGIETRFFEDRLGLDLTYYNMSTVDQIFRAPVSRTTGYSFKYINAGEISNK
                     GVELQLFVRPVATRNFDWRMDVNWAKNTSLVVDLGGIDNLQLASLQGGVSINATKGEP
                     YGTIRGSNFVYKDGQKVVNSLGRYVTSPTSNQVIGNINPDWTGGVTNSFRFKNVNLSF
                     LVDVKQGGQVWSLDMSYGLATGLYPETAGLNDKGNEVRLPTADGGGILLPGVKADGTP
                     NTTYASGYDFGIYGYRRNPNAAFVYDASFVKLREVNLSYDFPKSWLGAQGFLKGVNVG
                     IYARNLWTIHKNTPYSDPEEGFSSGNIQGYQGGAYPNVRVIGFNLGVKF"
     gene            6367..7791
                     /locus_tag="PLE23_05140"
     CDS             6367..7791
                     /locus_tag="PLE23_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HMX82685.1"
                     /translation="MKKYILLLILAGFAMSCNKDFDSLNTNTKKPKDVDPAFLFSGAQ
                     RQLSDVLTNNNVNFNIFRLTSQYWTETTYVDESNYDLNTRNIPQNFWNQMYVDVIKGF
                     ADSEAQLKRIDPNLSSADSKNKFACTEIMIAYAYSILVNTYGNVPYSEALNIDNVYPK
                     YDDAATIYSKLLERIDAALGSLDANAGSYGSGDLMMGGDIAKWKVFGNSLKLKLGMML
                     ADADPVTAKSVVSAAAPNVMASSGDNVIFHYFGSPPNTNPIWVDLVQSNRKDFVASQT
                     IVDAMNALGDPRISAYFTKDAAGVYSGGPQGASNNYATYSKPGNRLIAQDFEAIIMDY
                     SEISFLLAEAIERGFISGSAEQYYNGGIIESLRYWGITGTDATAYLQNPNVAYSTATG
                     DWKQKIGTQKWLALYNRGFDAWTEIRRLDYPVLPAPADPVTDFPVRYTYPVQEQNLNT
                     AKWTEAAAAIGGDDVTTKLFWDKF"
     gene            complement(7866..8315)
                     /locus_tag="PLE23_05145"
     CDS             complement(7866..8315)
                     /locus_tag="PLE23_05145"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HMX82686.1"
                     /translation="MGIDKKEKFIRTPVYLNGTAGIKQFVKENLKYPIEAFNAKIEAT
                     VHVKYIIDSNGDVIEAKTVGHVGYGLDEEAIRVVKLLKFSKVRNRNLRVTFNRKLDIH
                     FRMPGSTEIPAKMPQATGIHVQYTYTTPQSEQKEEKPGSFQYKITIK"
     gene            8431..8625
                     /gene="rpsU"
                     /locus_tag="PLE23_05150"
     CDS             8431..8625
                     /gene="rpsU"
                     /locus_tag="PLE23_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HMX82687.1"
                     /translation="MLIIDVKDSESIDKALKKYKKKYEKSGVLKELRRRKQFTKPSVI
                     RRTEVLKAEYKMQKYGTDAI"
     gene            8696..9583
                     /locus_tag="PLE23_05155"
     CDS             8696..9583
                     /locus_tag="PLE23_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMX82688.1"
                     /translation="MNIQRYLAHLEFEKRYSQHTLLSYKTDLRQFTDYLYNQYSITQL
                     AEVQNLHIRSWLVMLVNDGISARSVSRKLSTVKSLFKYLQLNKVLSINPAINLSAPRS
                     GKRLPGFVPEKEVISLLSQDLFADTPEAKQHRLILELLYTCGLRRSELINLEIKNIDL
                     GRNCIKILGKGNKERLVPIGRPLVESIVEFLNKRTVDGTQNNSYLFYNRKGNKLDPKS
                     VYNIVVRYLSMVTKTERKNPHILRHSFATHLIENGADLNSIKELMGHSSLAATQVYTH
                     NTIERLKDIYEKGHPRSGK"
     gene            9615..9914
                     /gene="raiA"
                     /locus_tag="PLE23_05160"
     CDS             9615..9914
                     /gene="raiA"
                     /locus_tag="PLE23_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HMX82689.1"
                     /translation="MKTRTEAIHFTADKKLLDFIEKKTSKLHQFFDRITDIDMKLKLE
                     NSGQVRDKIAEVKVQLPGTVLFVKEHDKTFETAIDAAVDSLAKQLIKYKERKSEN"
     gene            10204..10276
                     /locus_tag="PLE23_05165"
     tRNA            10204..10276
                     /locus_tag="PLE23_05165"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10237..10239,aa:Thr,seq:tgt)
BASE COUNT         3155 a         1904 c         2157 g         3061 t
ORIGIN      
        1 gctttggcga tatcaataca agcttgttgt taggttcaca aattaacaag agaaccttta
       61 atagtgtctt tgccagaggt cagttcctta caatacctaa cttctttaac ttgtcaaatg
      121 cttctgatct gtatgccgat caaagctctt cagtagtgaa gcttgcaggt gtgtttgcga
      181 gtttagacct tgattacaaa aactggattt atgtaaacct ttcaggtaga aatgactggg
      241 caagtacatt tggccctaaa gccaaaaaca gcttcttcta cccaagtgcc agtattgcat
      301 ttgtacctac agagattatg gcacagaatg atttcttgaa ttacttcaaa gtaagagcat
      361 cttttgctca ggctggtcgt gaaccaagtg cttactcttc tgctacttat tatacaaagc
      421 caacatttac agatggtttt acggatggta tctcattccc ttaccttggt aataatggtt
      481 ttggtatcag tggtacactg ggcaatgcag aattgagacc agagatcaac actactttcg
      541 aagtaggtgc agatttcaaa ttattgaaaa acagaatcaa cttcggtatt acatattaca
      601 attcaaaagc tactgatttg ttgattgcta gacctatagc aagctctaca ggttttggcg
      661 cattcgttgc taactctggt gagatgacca acaaaggtat cgaacttgag ttgggtatcg
      721 acatcgtaaa aacaaagact ttcaactgga atatagaagg caacttctca aagaatacca
      781 acaatgttga caaacttgtt gaaggtgttg atcaattcag tattgaggct gcctttacag
      841 gtgttggttc ttatgcgatt gttaatcaac cttatggtgc tatctttggt accaagtgga
      901 agagaaacga tgcaggccag ttgattattg gtgccaacgg acttccattg aaggaagcag
      961 aagaaggatt cctcggtaat ccttacccgg attggatttc tgctatcaga aatacattca
     1021 cgttcaaagg tttctccctt acagggcttc ttgacatcag acagggtggt gacatctggg
     1081 gtggtaccat cggccgtctg aacaatatcg gccgtacagc tgcttctgcc gacagaactc
     1141 atacttatgt aataccaggc gtgaaagctg acggtactcc caatgatatt gctatcagcc
     1201 ctaatttcta ctatgccaga tacctcggtg atgctggtgg agcaacagaa tccttcattt
     1261 ttgatggttc ttgggtaaga ttgcgtgaag tgactttgtc atattcatta cctaagttca
     1321 agaaattgat ccagaatgct tctgtgtatg ttacaggtag aaacctatgg ttgaagacca
     1381 agtatccggg tgttgaccct gagtcaagcc ttacaggtgc aggttctaat atcggaggat
     1441 ttgattattt cactatgccg ggttctaaat cgattattgt tggtttaaat atcggtttct
     1501 aattttttta ataaaaatta aaatgtcgaa aatgaaaaat tatatatttt tagcattgct
     1561 tcttaccggt cttgtattta caggctgtaa gaagtatgtg gagggagaag atatttcacc
     1621 aaactctcct tctaatgtaa cagaaggcct gttattgtcg ggtcttgagg ttgcaacatt
     1681 ctccaatttt ggaggcgatt tgtcacgtgt tacttcagtg cttacacaac aatcaacagg
     1741 taatctattt cagtacgaag atcttgcaaa gtacgacatt accgaatcta ctgttagtaa
     1801 tatatggaat cagctttatt caggttcctt gatcaatgca cagttgctga ttgataaagc
     1861 cggagataaa aaccgtcatt atagaggtat aggcagagta ctcaaggcat tgaaccttgg
     1921 acttgcaaca gatttgtggg gtgatgtacc gaataaagag gcattaggtg gcctgaacgg
     1981 caattttaat gcggcatttg attcacaaga gtctgtaata gctgacatac aacagacact
     2041 agatggtgcc atcgcagacc tccagtcaac tgagaacgtt atagcaccag gtgctgatga
     2101 ttatattttt ggcggtgatg cttcaaaatg gcttatgaat gcttatttgt tgaaggctcg
     2161 ttatgctaac aggcttagca aaaaggatgc tacaggctca gccaacaaag cacttgaagc
     2221 attgaatggt gcttatgctg caggttatac aggtaatggt agcaaccttt atgctatctt
     2281 tggtccaggt gtgactgaga gcaatcaatg gtatgtgttc aatcaggacc gtgcaggtta
     2341 cgtttcaatg ggtagcacac ttgtaggaat gatgtctgcc atcaatgacc caagacttgc
     2401 attctatgca gctaaagatg gtagtggaaa ttattcaaat gaatcttcca ttggtgcaaa
     2461 atatggttca gccgaatcaa agctaccttt gcttacttat gcagaagcaa aatttatcga
     2521 agcagaagct caaatgcgat tgggcaataa ggctgcagca gctgctgcac acaatgctgc
     2581 tgttattgca aacttaaatg atgttaccgg tggtggcgat gctgctttcg aagctgccaa
     2641 tgcaaacgaa gatgcagcta gcatcactat gagtaagata atgtctcaga agtatattgc
     2701 tatgtttaca caaccagaag tatattctga ctggagaagg acaaatattc ctgcccttac
     2761 tccaaatgcc ggagcaaacc ttactgagat accgagaaga ttccctacag ctcaagatga
     2821 aagattatac aataccaaag ctgttgtaaa ctctaacctc cttgaccctg tatggtggga
     2881 taaataagca tcagtaattc tgatcgtaat ttttatatgg ctatccattc acttggatag
     2941 ccatatttta ttttgtagaa gtgttaaaaa ttttatataa attccgaact gtaaattgtg
     3001 atgaaagttt atagataaaa atttgtattt tctatgatac atttaaaata ctgattatat
     3061 ttgattcttg aaaaaacaat tttatcacac aaaaagagga ttagtatgaa aaaacttaca
     3121 cttctgattt ctatgttgct aatagcaagt gcttcgctgt tagcccaaag gacagtcgtc
     3181 ggaaaagtta ccgattctgc cggaagtcca cttccaggtg ctacagtact tgtaaaaggt
     3241 acaagtgaag gtacagctgc cgacgacaat ggtaacttca gattgaacgt tcctgcaaat
     3301 gccaacacac ttgtagtttc gtacacaggc tttaacacgc aggaagttct attaggggca
     3361 tctaatgttg ttaatgttgc cctgcaggaa ggggtgaccc tttctgagac agttgtaacc
     3421 gcattgggta tcactaagaa gaaaaatgag ctaccgtatt ctgcacagac ggtaggtgga
     3481 gatgatgtct ccaaatccag agacggcaac gttgtgaatg ccctgtcagg gaaggttgca
     3541 gggttaaaca ttaagcgcaa caacagtctt ggcggttcta ccaatattgt tttgcgaggt
     3601 accaaatctt tgacgggcaa caatcaggct ttgtttgttg tggatggtgt gcctgtagat
     3661 aattcaaaca caaatacttc tgaccagcta acaggccgtc agggttatga ctttggtaat
     3721 gctgcagccg acatcaattc tgacgatatt gacaatatta cagtactaaa gggagcagct
     3781 gcttcagctc tttatggttc gcgtgctgca aatggggtaa tcgttataac cactaaaaag
     3841 ggctccaaag ccaaaggtct tggtgttact ttaaattcag gcttgaatgt cggtaccatt
     3901 gagaaaagta cctttgctac gtaccagaag aaatatggag ctggttatgg tgcatattac
     3961 gaagacccat caggcttttt cttatacaga gacattgatg gtgatggtac agatgatctt
     4021 gtgacacccc ttacagaaga tgcttcctgg ggcgctaagt ttgaccctaa cctgaaagta
     4081 ttccagtggg atgcttttga cccaacttcg cctaattaca aaaaagctac accttgggtg
     4141 gcagcagcaa atgacccttc tacatttttc gaaacagcca tcggtaccaa taatggtgta
     4201 atgatagatg gaagtaatga caaaggttat tttaaactcg gttataacca tattacggat
     4261 aaaggtatcc tgccaaatag taatatcaat aaggatatga ttaactttgg tggttcgtat
     4321 aacctaaccc gcaaactgac tgcttattct gcaattaact ataccggtat aagtgcgaag
     4381 ggtcgttatg gaacaggcta tggtgacagg aaccttatga ccaacttcag acaatggtgg
     4441 caaaccaatg ttgatatctc agaattgcgt caagcgtatg atagaacaaa acaaaacgta
     4501 acatggaact gggcagaccc aactattctg gcaccgatat actgggataa cccttactgg
     4561 acaagatacg aaaactacga aacagacggc agaggtcgtt atttcggtaa tgttggcctt
     4621 gagtacaaac tcctaagctg gttgaatctt aaaggtcaga tgtctctcga tagatatagc
     4681 gaataccagg aagaacgtat agcggtaggt tctgttgatg taccgaaata ctcaagatat
     4741 gaccgtacat tccaggaaat taattacgat ctcttgttaa gttctacacc attgcgcctt
     4801 tccgagagtt tacatttcaa tttccttctc ggttccaatg ttcgcaagaa tgaaatttat
     4861 gctattgctg atggtaccaa tggcggtctt gctattcctg gtatctatgc actgtccaat
     4921 agcgtgagtg cccgaaatgc accagacgaa tcactcacca aactgcaagt gaatggtctg
     4981 tttgctcaaa caagctttaa ctgggataac agcgtgttcc ttgatttatc agtaagaagg
     5041 gacaaatctt ctgccttgcc taaggataac aatgtatata tctatcctgc tgcttcactt
     5101 ggtttgttgt tttctaactg gacaggtaca ggttctgtat tctcgttcgg aaaattgaga
     5161 ttcaactatg cacaggttgg taatacccca ccaccactcg tagtggatga tgtgtataac
     5221 atcggtgtgg ccaccctact tgacagaggt gtgtatgcta catcatttgg ctcggcacct
     5281 ttagcttctg ttagttctac taagaacaat ccgggcctga agcctgaaag tacaaagagc
     5341 tttgaggtag gtatcgaaac aagattcttt gaagatcgac taggtcttga ccttacatat
     5401 tataatatga gtaccgttga ccaaattttc agagcccctg tttctcgaac tacaggctat
     5461 tcattcaaat acatcaatgc aggagaaatt tctaacaaag gtgtagaact tcaattgttc
     5521 gtacgtcctg ttgcgacacg taattttgat tggagaatgg atgttaactg ggcaaagaat
     5581 acaagccttg ttgttgacct tggtggtatc gataatcttc agcttgcatc attgcagggt
     5641 ggcgtatcca tcaatgctac taaaggtgaa ccttacggta ccatcagagg aagcaacttt
     5701 gtgtacaaag atggtcaaaa agtagtgaat tctcttggac gatatgtaac aagccctaca
     5761 tccaatcagg taatcggtaa tataaatcct gactggactg gtggtgttac caatagcttc
     5821 cgtttcaaga atgttaacct tagcttcttg gttgatgtca agcaaggcgg tcaggtttgg
     5881 tcattggata tgagctacgg tcttgctaca ggtctctatc cagaaacagc cggactaaac
     5941 gataaaggaa atgaagtgcg actaccaact gcagacggag gtggtatcct tcttcctggt
     6001 gttaaagcag acggaacccc gaatactact tatgcaagtg gctatgattt tggtatctac
     6061 ggttacagaa gaaaccctaa tgctgcgttt gtatatgatg ccagctttgt gaagttaaga
     6121 gaagtaaatc tttcttatga tttccctaaa tcttggttag gtgctcaagg cttccttaaa
     6181 ggtgttaatg taggcatcta tgcacgtaat ctttggacta tccataagaa tacaccgtac
     6241 tcagatcctg aagaaggatt cagctcaggt aatattcagg gatatcaggg tggtgcttat
     6301 cctaacgtga gagttattgg tttcaatctt ggagttaagt tttaataaac aagcaaaata
     6361 tttgttatga aaaagtatat cttattattg atattagctg gttttgcgat gagttgcaac
     6421 aaagacttcg actcattgaa taccaacaca aaaaagccta aagatgttga cccggcattc
     6481 ttgttttcag gtgcccagag gcaattgtca gacgtcctga cgaataacaa tgtcaatttc
     6541 aatattttca ggcttacatc acagtactgg acagagacta cttatgttga tgagtcaaac
     6601 tatgatttga acaccagaaa tatccctcag aatttctgga atcagatgta tgtagatgtc
     6661 ataaaagggt ttgcggatag tgaagcacaa cttaagcgaa ttgatccaaa cttatcatct
     6721 gctgacagca agaataaatt tgcctgcaca gagattatga ttgcttatgc atattctatc
     6781 ctggtaaata cgtatggcaa tgttccgtat tctgaggctt tgaatattga taatgtatat
     6841 ccgaaatatg atgatgcagc taccatttat agtaaattac tcgaaagaat agatgctgca
     6901 cttggctcac ttgacgcaaa tgctggttcg tatggcagtg gtgaccttat gatgggaggt
     6961 gatattgcca agtggaaagt atttggtaat tcgctaaaac tgaagcttgg tatgatgctc
     7021 gctgatgctg accctgtaac cgcaaagtcg gttgtgtctg cagctgcacc aaatgttatg
     7081 gcatcttccg gtgataatgt tattttccat tattttggtt ctccaccgaa tactaatccg
     7141 atttgggttg atttggtaca aagcaacaga aaagactttg tagcttcaca gaccatagtt
     7201 gatgcaatga atgctttggg cgatccaaga atttcagcat attttaccaa agatgctgca
     7261 ggtgtatatt caggtggccc acaaggtgcc agcaacaatt atgctaccta ttctaagccc
     7321 ggcaatagac tgattgctca ggattttgaa gccatcatca tggattattc cgaaatctct
     7381 ttcttgcttg cagaggccat tgaaagaggc ttcataagcg gtagtgccga acaatactac
     7441 aatggaggta ttatagagtc tttgagatat tggggcataa ccggtactga tgcaactgct
     7501 tatctgcaaa acccaaatgt cgcatattct actgcaacgg gtgattggaa acaaaagata
     7561 ggtacgcaga aatggcttgc attatataac agaggtttcg atgcctggac tgaaatacga
     7621 agacttgatt acccggtact tcctgcacca gccgatcctg ttacagattt ccctgtccgt
     7681 tatacatatc cggtgcagga acaaaatctt aatactgcca aatggactga agctgcggca
     7741 gccattggag gtgacgatgt aaccactaaa ttattctggg ataaattcta agttctgaat
     7801 ttgttctggt tttgttctgt tttatatctt gccgtcatca atgatcagat ggcggcaagt
     7861 cttttttact tgattgttat tttgtactga aaactgcctg gtttttcttc cttttgttca
     7921 gattgaggtg ttgtataggt atattgtaca tggataccag tagcttgggg catttttgcc
     7981 ggtatctcag tgctgccggg cattctgaaa tgaatatcga gttttctgtt aaaagttacc
     8041 cttaggttgc ggttgcgcac tttcgaaaac ttcaatagtt ttactaccct tatagcttct
     8101 tcatcaagtc cgtagcctac atgacctact gtctttgctt caatgacatc accattcgaa
     8161 tcaataatgt atttgacatg aacggtagcc tcaattttag cattaaatgc ctctatcgga
     8221 tattttaaat tttcctttac aaattgtttt ataccggcag taccatttaa atagaccgga
     8281 gtacgaataa atttttcttt tttatcaatg cccatttctc gttttataaa aatatacgca
     8341 aataaaaaat aaattcatgc aaataatttg gattattaat tacaatctcc ctatttttgc
     8401 actctaattt atttaaaatt aaatcagtat atgttaatta ttgacgtaaa agacagcgaa
     8461 tcaatcgata aggcactaaa gaagtacaaa aagaagtacg aaaaatctgg tgtgttaaaa
     8521 gaattaagaa gacgtaagca attcacaaag ccttctgtta tcagaagaac agaggtgctg
     8581 aaggctgaat acaaaatgca gaaatacggc acagacgcga tttaaatttt tctatttaaa
     8641 tatatctttg tactttaggg tctgattatg attattacat aatcagacct tttttatgaa
     8701 cattcaaagg tacctggcac atttagagtt tgagaaaaga tattctcagc atacgctcct
     8761 ttcctataaa actgaccttc ggcagttcac agactacttg tacaatcaat attcaattac
     8821 acagttagca gaagtacaaa atctccatat ccgttcatgg ttggtgatgc ttgtgaacga
     8881 tggcatctca gcacgttctg tatcacggaa gttgtcaact gttaagagtt tgtttaagta
     8941 tcttcagctc aataaagtac taagtattaa cccggcaata aacctttctg caccaagaag
     9001 tggcaagcgc cttcccgggt ttgtacctga aaaagaagtt ataagcttat tatcacaaga
     9061 tttatttgcg gatacgcctg aagctaagca gcaccgtttg atccttgagt tattatacac
     9121 ttgtggtctt agacgaagtg agcttatcaa tcttgaaata aagaatattg atctaggaag
     9181 aaattgtatt aagattttag gcaaaggtaa caaggaacga ctcgtgccca taggcagacc
     9241 attggtcgaa tctattgtag aattcttaaa taaacggact gttgacggaa cacaaaacaa
     9301 ttcatacttg ttttataata gaaaaggaaa taaattggac ccgaaaagcg tttataatat
     9361 tgtagtgaga tacctgagta tggtgacgaa aacagaacgt aagaatccgc atatattaag
     9421 gcatagtttt gcaacccacc tcatagaaaa tggtgccgac ctcaattcta ttaaagagct
     9481 aatggggcat tcgagccttg cagctacaca ggtttataca cacaacacaa tcgaacgact
     9541 aaaagacatc tacgaaaagg ggcatcccag atcgggtaag taagagattt tttataacat
     9601 ttaaataaaa agatatgaaa acaagaactg aagcgattca ttttacagca gacaagaagt
     9661 tgttggactt tattgagaag aaaacatcca aactgcacca gttttttgac cgcattaccg
     9721 acatcgacat gaaattgaag ttagagaatt cgggtcaggt acgggacaag atagcagagg
     9781 taaaggtaca gttgcccggt acagtactat tcgtaaaaga acatgacaaa acattcgaga
     9841 cagcaataga cgcagccgtt gattcacttg ccaaacagct gataaaatat aaggagagaa
     9901 agagcgaaaa ttaaaaaatg gaggccggaa cttatccggc ttcttttttt ttaattaagt
     9961 gcaacgattt ataaaaagct acgttaaatg atactcaact tcaataaaag tagtgccggc
    10021 aaaaaaaagt aaaaatattt ttcaaaaatt gatatttttt tttgtatttg ttgatagaat
    10081 ttatatcttt gcatcgcttt tggaaaaaag tttctgaaag taattgaaag ttaagcaaat
    10141 aagtaaaagc tacttgctta atcaaattga aaaatagggc aatattttat aaaaaatatc
    10201 caggccagtg tagctcagtt ggtagagcag ctgatttgta atcagctggt cgggggttcg
    10261 agtccctcca ctggctc
//
LOCUS       DAOQQY010000071        10260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2380_length_10260_cov_6.894855, whole genome shotgun sequence.
ACCESSION   DAOQQY010000071 DAOQQY010000000
VERSION     DAOQQY010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2380_length_10260_cov_6.894855"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..1739
                     /gene="tig"
                     /locus_tag="PLE23_05170"
     CDS             285..1739
                     /gene="tig"
                     /locus_tag="PLE23_05170"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HMX82690.1"
                     /translation="MIDIVRQDIEGQQANISITIPVEDYRPKWEAELAKAQKKAQLKG
                     FRKGKTPLSYVKKLYGEPLLSDIVNEIIQKEIQDYLVREKIHYLGQPIPSEGQELLNL
                     SVDNMKDLTFKFDIGLEPEGEIKGLGQSVTLPYDETTIPAEMVDAELKALRAQAGERV
                     DTDLKIEKGDLVTVSAKEMDKGKEKKNGWQVPITFLVDDIPGEELKTTLLSKKKGDTV
                     EFKITEIEQNDDPAHIRKHLLQLDANDTRDVNEEFAGVIDEVSRLQDAELNQEFFDKA
                     FGKDEVQSEEEAKELIRRTLKRRYDYTADSLLFREIQTKLMEENNMTLPDAFLKRWLM
                     ITNQDVTEDAINSEYDMFAKNLQWTIIKNKLNKKYGIEVTPEEIKEGFRNYYSSYFGM
                     QLPDNMLNTFLEKVLQKQDQVEKMYDDLMIDKLFKNLKAEFKLDLKEVSIDDLTSKAQ
                     ALQHKHEHSHDHDHEHDHNHDHDHHHDHDHDHHH"
     gene            1904..2614
                     /locus_tag="PLE23_05175"
     CDS             1904..2614
                     /locus_tag="PLE23_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMX82691.1"
                     /translation="MIPQDEFKKYATQHLGMSSMHLEKYMQVSAPNIHAFTPQVIEER
                     SLNVVGMDVFSRLMMDRIIFMGVPVTDYVANVIQAQLLFLESTDPKRDIQMFINSPGG
                     SVIAGLGIYDTMQYVTPDIGTICTGLAASMGAVLLAAGAKGKRTCLYHSRVMIHQPMG
                     GMQGQVTEMEISYNLIKHLQKQLYDILAYHTGQPYDTINKDCDRDNWMTADEAKAYGL
                     VDEVLERNNPRKKEDDKK"
     gene            2601..3842
                     /gene="clpX"
                     /locus_tag="PLE23_05180"
     CDS             2601..3842
                     /gene="clpX"
                     /locus_tag="PLE23_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HMX82692.1"
                     /translation="MTKNKREDNNCSFCGRPKAEAMMLVTGLHARICDICAEQAFAIV
                     QEETRKISTKSKNADLDLGGRTPKDIKSYLDEYIIGQDEAKKVLSVAVYNHYKRLGQQ
                     SSDDVEIEKSNILFVGPTGTGKTLIAKTIARLLNVPFAIVDATVFTEAGYVGEDVESI
                     LSRLLQVCDYNVAQAEKGIIYIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEGAD
                     VLVPPQGGRKHPEQKLISVNTRNILFMCGGAFSGLDKIIARRVNTQVIGYKNQEHTEA
                     VKENMLQYVNHIDLRSFGLIPELIGRLPVLVHLNDLDKSAMIKILTEPKNSLIKQYRK
                     LMEMEGIELEFTDDALDLIADTAIEFKLGARGLRSICESVLTDAMYELPGNKKIKRFV
                     IDKEYVGSKLSESKIHKLKAA"
     gene            3845..4444
                     /locus_tag="PLE23_05185"
     CDS             3845..4444
                     /locus_tag="PLE23_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82693.1"
                     /translation="MTFTINIYLRFALIALGIIGGIALWAAYGFWYGFFFLLAGLILL
                     AGYFMLGTVQSAAMLMQSMNFDAVEKRLSLTFFPGMLYKANRAYLYMIKGTMAMYKKD
                     FNKAETFLQKAQDTGLATDNEKSTVYFQLANIAAQKNNWVGAQNQFKKAKEYKVTDPE
                     MKEQYRQFEKALANRGAMNPANRGMMIQPGGKRKRPRGM"
     gene            complement(4527..8996)
                     /locus_tag="PLE23_05190"
     CDS             complement(4527..8996)
                     /locus_tag="PLE23_05190"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMX82694.1"
                     /translation="MKLFYKIFQTSYCLLFFVFITGLQYTKAQVIINEFSAANYDNYL
                     CSNNDYEDWVELYNMGAAPVNLQGYYLSDRVDKPTKWQIPAGYILSPGDRILIIASGE
                     DNVIGGFLHTNFKVTQTDAKEAFVLASPSGAVIDFHEIDIPNGKNHAYARMPDGGPWT
                     VTNNPTPGAANNSTTTRYALTPQFNIQPGYYAGTQSVTLTTTEPNSTIRYTLDGSEPT
                     AASTAYSAPINVSTTRIIKARTYTSDPKILTSHTAFGTYFIGAASKHTMMVVSISGDD
                     VPLLLSGTQNNPQGTFELFDQNGKFIDACTGEFNKHGNDSWAYAQRGIDYVTRDQFGN
                     DSEIKHKIFPNTKRKKFQRIILKAAANDNYPALGTGAHLRDAYVHELSQRAGLDLDVR
                     STNFCVLYVNGNYWGVYDIREKVDDKDYTDYYYGLDEKEIDFIKTWGSTWAEYGTTAN
                     WFTLRNYILTNDMTNPVKFQYVADRLNLQSLIDYYIINVHTVCKDWLVWNTAWWTGMD
                     NEGNFQKWRYTLWDMDAVFGHYINYTNIPNVNADADPCDIETVGPSYDPQKHTEVLTK
                     LFTNKSFFDLYINRYADLNNTFLSCQYMTNLLNEMEAQLEPEMPGQIARWGGNFTTWK
                     NNVKAIRDFINERCVKIETGIVDCYPVQGPYNLVVVVKPEGSPNDVKVNTVIPDQYPF
                     NSKYYGGTDLHFTAMPGTGWDFEKWETKKMPINPNQTSTDMTSSVSEPDTLFAYFVIE
                     CPMVLGVKPTGQNYLDCKTGTIQLQAESTNGSGSYTYSWLDSKKNIIPGSNAAVIDIT
                     QAGWYYATVEDVINGCSRTDSFLVLDKKIYPNAIASVNDKLTCTNLAVDFITTGTDTG
                     PAFSYQWSGVCLEASASSLSNKACAPGQYTLVVTDNSNGCTALVNLQVDQDIKKPDDV
                     SINAASVNLPCDGSGVLLSVSGNGPNGNYTQIWSTKTGNILGDPTSNVVNANASAWYF
                     LTITNPENGCTHTDSILLKNNNTFLSSLSFNLKNPSCDLASNGEISVVAQGGGTPFTY
                     SLNGSALSAVSTFGNLTPGTYTILIENSDKCQFDTTISIIVVENLPQYPGIKTIGASC
                     DVAQNGGLEFTSQTGGFGPYTYSLNGSAYSTQTVYSNLAPGIVGISVKDDAGCIASYN
                     VAVPVSEDLPDQFATLPDGPNCSTASNGSLEINNISGGVPPYEVSFNNGPFGNILTAS
                     NLSAGDIQISIKDALGCIFNTVITLNADENLPSDYKVKKSEPACENGTNGKIEISNIS
                     GGVGPYQVTLNGITSPSGDDVFPSLTVGIQKLLITDALGCTYEDDILIKAGGVLVVDL
                     GEDLLVDDGSSVPVTATIYGNDITNISWTPEICLACTSAVVTVLSDTTLKVTVTDIGG
                     CSTEDELVIKIKENVDLYAANIFSPNGDGYNDTWRLQTSNPFAILKQLAIFDRWGGMM
                     FTASGGHPSADASAWDGTVKGKKCPAGVYVFMAEVTVKPGRTVLLKGEIAITR"
     gene            9163..10005
                     /locus_tag="PLE23_05195"
     CDS             9163..10005
                     /locus_tag="PLE23_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMX82695.1"
                     /translation="MRETTELKKIATQVRRDIVRMVFDAQSGHPGGSLGCADFMTALY
                     FSVMNHNPEFKMKGLGEDIFFLSNGHISPVFYSVLARSGYFPVSELATFRKLNTRLQG
                     HPTTHEGLPGVRVASGSLGQGLSVACGAALAKKMDNDSSIVYALTGDGELQEGQIWEA
                     AMFAAHHRIDNLIVTVDWNGQQIDGSNNEVCSLDVLPAKWHAFGWDVLQTNGNDMDVL
                     IHTLQEAKKACNRGRPIVILMKTEMGCGVDFMMGSHKWHGIAPNAEQLEKALAQLPET
                     LGDY"
     gene            10034..>10260
                     /locus_tag="PLE23_05200"
     CDS             10034..>10260
                     /locus_tag="PLE23_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HMX82696.1"
                     /translation="MSVNNLTVNGKKATRDGFGDGLTELGRNNPDIVALCADLLGSLK
                     MDQFKKENPERFIQVGIAEANMMGIAAGLAT"
BASE COUNT         3107 a         2105 c         2320 g         2728 t
ORIGIN      
        1 ggatagaagc cggcacgaag taaggcgcat agcccgaccc gacaggggca cgcccaaata
       61 caacaacacc ggatgcctaa aatatttgga ttaataaaaa tgtgtgataa aaaaaatccg
      121 gggcatcaca cgttacagtt gcgcagatgt gagaaatatt agcttgatgg cgcaaaataa
      181 agggaatttg aaattatata acatagtttt ttcatcgcta ttcaaaatct tataacgata
      241 tttgcggcct ctaaaatttc agtaaacaca atacgtataa aattatgata gacatcgtcc
      301 gacaggatat tgaaggccag caggccaata tcagcatcac catacctgta gaagactaca
      361 ggccgaaatg ggaagcagaa ctcgccaagg cgcaaaaaaa ggctcagcta aaagggttca
      421 ggaaggggaa aactccgctt tcttacgtta agaagcttta cggtgaaccg ctgctgagtg
      481 atatagtcaa tgaaataatc cagaaagaaa tacaggatta tctggtgcgt gaaaaaatac
      541 attatctggg ccagccgata ccatctgagg gacaggaatt gctcaatctg agtgttgaca
      601 acatgaagga tctgacgttc aaatttgaca ttggtcttga acctgaaggt gaaataaaag
      661 ggctgggcca aagtgtaaca ctaccttatg atgagactac gatacctgct gaaatggttg
      721 acgctgagct gaaggcactg agagcacagg ctggcgagcg cgtcgataca gacctgaaga
      781 tagaaaaggg cgacctagta actgtaagcg caaaggaaat ggataagggg aaggagaaaa
      841 agaatggttg gcaggtacct attacctttc tggtagatga cataccgggc gaagaactga
      901 aaacgacgct actgagcaag aaaaaaggag atactgtaga gtttaaaata acggagatcg
      961 aacaaaacga tgatccggca cacataagga aacatctgct gcagctggat gcaaatgata
     1021 cgcgcgatgt aaatgaagaa tttgcaggtg tgatagatga ggttagccgc ctgcaggatg
     1081 ctgaactgaa ccaggagttt ttcgacaagg ctttcggcaa ggacgaggtg caatcggaag
     1141 aagaagctaa ggagttgata aggaggacac tcaagcgcag atatgattat acagcggact
     1201 ctctgttgtt cagagaaata cagaccaagc tgatggagga aaacaatatg accctgcctg
     1261 atgcgttctt aaaaagatgg ctgatgataa ccaaccagga tgtgaccgaa gatgctatca
     1321 atagtgagta cgacatgttt gccaaaaacc tgcagtggac catcatcaag aacaagctga
     1381 ataaaaaata cggcatagag gtaacacctg aagaaataaa agagggattc aggaattatt
     1441 attcgagcta cttcggtatg cagctacccg acaatatgct taatacattc ttagagaaag
     1501 tacttcaaaa acaagatcag gtagagaaga tgtatgacga tctgatgatt gacaaattgt
     1561 tcaagaacct gaaggcagaa tttaagctgg accttaaaga agtatccatc gatgatctga
     1621 ccagtaaagc acaggcattg caacacaagc atgaacatag tcacgatcat gatcacgagc
     1681 atgatcacaa tcacgaccat gaccatcatc atgatcacga ccacgaccat catcattaaa
     1741 aagtacagat gtgtggaaca cgagtcatcg aaccgaatgg cagaaggatt gttgtagtaa
     1801 atgacagaaa tggtctgaag cacacaagca gagtagtatc ggtaggtgtg aaggttgttt
     1861 aggttttatg aaaaaataat tttaataatc aatcaaaaga aaaatgatac cacaggacga
     1921 atttaaaaaa tatgctaccc aacaccttgg catgagcagt atgcatttgg aaaaatatat
     1981 gcaggtgtct gcacccaata ttcatgcctt tacgccacag gtaatagagg aaaggtcgct
     2041 caatgtagta ggtatggacg tattcagtcg tctgatgatg gacagaatca tcttcatggg
     2101 tgtaccagtg acggactatg tagcaaatgt tatacaggcg cagttgttgt ttttggaatc
     2161 aactgaccct aagcgcgaca tccagatgtt catcaacagt ccgggcggtt ctgtcattgc
     2221 gggacttggt atttatgaca ccatgcagta tgtaacgccg gatatcggaa caatctgtac
     2281 cggtctagcg gcatcgatgg gtgctgttct gttggcagca ggtgctaagg gcaagcgtac
     2341 atgtctatac cacagccgtg tgatgataca ccagcctatg ggcggcatgc agggccaggt
     2401 aacagagatg gaaatcagct acaatctcat caaacacctt cagaaacaac tgtatgatat
     2461 actcgcatac cataccggcc agccttatga taccatcaac aaagactgtg accgcgacaa
     2521 ctggatgact gcagacgagg caaaagctta cgggctcgta gatgaagtac ttgaaagaaa
     2581 taatcctcgc aagaaagaag atgacaaaaa ataagcgcga agataataat tgctcttttt
     2641 gtggcagacc caaggctgaa gccatgatgc tggtgacagg gctacatgcg cgtatctgcg
     2701 atatatgtgc tgagcaggca tttgccatcg tacaagaaga aaccagaaaa atttctacaa
     2761 aaagtaaaaa tgccgatctg gaccttggcg gcagaacgcc aaaagacatc aaaagctacc
     2821 tagacgaata catcattggt caggatgaag caaagaaagt actgtcggta gcagtatata
     2881 accattacaa gaggctaggc cagcaaagca gtgacgatgt agagattgaa aaatcgaata
     2941 tcctcttcgt cgggccaacg ggtaccggaa agaccttgat agccaagacc attgcaagat
     3001 tgctgaatgt gccatttgct atagtagatg ctaccgtatt tacagaagca gggtatgtgg
     3061 gcgaagatgt ggagagcata ctcagcagac tacttcaggt gtgcgactac aatgtagcac
     3121 aagcagaaaa aggcatcata tatatagacg agatagacaa aatagcccgc aagagcgata
     3181 atccttccat aacaagggat gtgtcgggtg aaggtgtaca gcaggctttg ctcaaactgc
     3241 ttgaaggcgc cgatgtactg gtaccgccac agggaggccg caaacatccg gagcaaaagc
     3301 ttatttcggt caatacgcgt aacatattgt tcatgtgtgg tggtgcattc agtggacttg
     3361 acaagattat tgcacggcga gtgaacacac aggtcatcgg ctacaaaaat caggaacata
     3421 cggaggcagt taaagaaaac atgctgcagt atgtcaacca catcgatctt cgcagttttg
     3481 ggctcattcc ggaacttatt ggccgactgc ccgtactagt tcatctgaat gatctggaca
     3541 agtccgcaat gatcaaaatt ctgacagagc caaaaaattc tctgataaaa caatacagaa
     3601 aattgatgga aatggaaggt atagagctcg aatttacaga tgatgcactt gacctcatag
     3661 cagatacagc catagagttc aaactcggag caagagggct tcgttctatc tgcgaatcgg
     3721 tacttactga tgctatgtac gaactacccg gcaacaaaaa aatcaagaga ttcgtaattg
     3781 acaaggagta tgttggtagc aaactttcag aatctaaaat acacaaatta aaagcagcct
     3841 gattatgaca tttactatta atatttacct tcgttttgca ctcatagccc ttggcattat
     3901 agggggcatt gcgctgtggg cggcctatgg tttttggtac ggctttttct tcctgcttgc
     3961 cgggttgatt ctattagcag gatactttat gcttggcaca gtacagtctg cagctatgct
     4021 catgcaatca atgaattttg atgctgtaga aaagaggctt tctcttacat tcttccccgg
     4081 tatgctgtac aaggctaacc gtgcatatct gtatatgatt aagggtacga tggctatgta
     4141 caaaaaagat ttcaacaaag ccgaaacatt ccttcaaaaa gctcaggata ccggccttgc
     4201 cactgataat gaaaagagta cagtatattt tcagctggca aatattgcag ctcagaaaaa
     4261 caactgggta ggagcacaaa atcaattcaa gaaagctaag gaatataagg tgaccgatcc
     4321 ggaaatgaag gaacagtaca gacagtttga gaaagctctg gccaaccgtg gtgctatgaa
     4381 tccggccaac aggggcatga tgatacaacc tggcggtaag agaaaaagac cacgaggcat
     4441 gtaaactgtt tcaaatttcg ctcagtataa taaaaaccca tctccgagac agaagatggg
     4501 ttttttgttg aatatccgga cctaaattac ctagtaatgg caatctcacc tttgagcaat
     4561 accgtccttc cgggcttgac cgttacctca gccatgaaca catacactcc ggcagggcat
     4621 ttctttccct tgaccgtacc gtcccaggct gaagcatcag ctgaagggtg gcctccggag
     4681 gcagtaaaca tcatccctcc ccatcggtca aatatagcca gttgtttgag tatagcgaaa
     4741 ggattggatg tctgcagcct ccaggtatca ttgtatccat caccgttggg tgaaaagata
     4801 ttcgcagcat acagatcaac attctccttg atttttatga ccaactcgtc ttccgtggaa
     4861 cagccgccta tgtctgtaac agttactttg agtgtagtgt cagacagaac agttacaact
     4921 gcactcgtac aagccagaca aatctcaggc gtccaggata tgtttgtaat atcatttccg
     4981 tatatggtag ctgtcacggg tacactcgat ccgtcatcta caagcaggtc ttctccgaga
     5041 tcaaccacca atacaccacc cgccttgatc agaatgtcat cttcgtaagt acagcccaaa
     5101 gcatccgtta tcaacaactt ttgtatgcct actgtaaggg aagggaaaac atcatctccg
     5161 gatggtgaag taataccatt gagtgtcacc tggtaaggac ctacgccacc tgatatgttg
     5221 cttatttcta tctttccgtt tgtaccattt tcacatgcag gttctgattt ttttacttta
     5281 tagtccgaag gtaaattttc atctgcatta agggttatga cggtattgaa aatacatccg
     5341 agtgcatctt ttattgaaat ctgaatatcg ccagccgaca gattggatgc tgtgagaata
     5401 ttgccaaagg ggccattgtt gaaacttact tcatagggcg gcacacctcc tgaaatgtta
     5461 ttaatttcga ggctaccatt tgaggcagtg ctgcaattgg ggccatcggg cagggtagcg
     5521 aactggtcag gcaaatcttc actcaccggt actgccacat tgtaactggc tatacatccc
     5581 gcatcgtcct ttactgatat gcctactata cccggtgcga gattagaata aactgtttgg
     5641 gtagaataag cgctaccatt caggctataa gtatatggcc cgaaccctcc ggtctgtgaa
     5701 gtgaattcta accctccgtt ttgagctaca tcacatgaag caccgattgt tttaattccg
     5761 ggatattgtg gcagattttc gacaacaata atcgaaatag tcgtgtcaaa ctgacattta
     5821 tctgaatttt cgattagaat ggtataagtg ccgggcgtaa gattgccgaa ggtagaaacc
     5881 gccgaaagtg cagaaccatt caatgaatag gtaaatggtg taccacctcc ctgagcgaca
     5941 acactgattt cgccgttaga ggcgagatcg caactcggat ttttcaggtt aaaactcaat
     6001 gaagacaaga atgtattgtt gttctttaac aagatactgt cagtgtgtgt gcaaccattt
     6061 tcaggattag ttattgtgag aaaataccaa gctgaagcat ttgcattcac aacatttgaa
     6121 gtcgggtcgc ccagaatgtt tcctgtcttg gttgaccaaa tttgggtata gtttccgtta
     6181 gggccgttac cactaacact gagtagcaca cccgaaccat cacaaggtaa atttacagaa
     6241 gcagcattta tcgatacatc atcaggtttt ttaatatctt gatctacttg taagtttact
     6301 aaagctgtac atccgttgga attatccgtt actaccaatg tatattgacc aggggcacag
     6361 gctttattgc ttagagacga tgctgaggct tcaagacaaa cgcccgacca ctgataagaa
     6421 aatgcaggac cggtatccgt gcctgtagtg atgaaatcaa cagcaagatt ggtacaagta
     6481 agtttatcat tcacagaagc aatggcattt ggataaatct ttttgtcgag taccaaaaag
     6541 ctgtcggtac ggctacaacc attgataaca tcttcaacag tagcataata ccatccggcc
     6601 tgtgttatat caattactgc tgcattgctg cctggaatga tgtttttttt cgaatcgagc
     6661 catgaatatg tgtagcttcc actcccattc gtgctttcag cctgaagctg gatagtaccc
     6721 gttttacaat ccagataatt ctgtccggta ggtttcacac ccagcaccat tgggcattcg
     6781 atgacaaaat aagcgaaaag tgtatcgggt tctgacacag atgatgtcat atcagtactg
     6841 gtctgatttg ggttaatggg catttttttt gtttcccatt tttcgaaatc ccatccggta
     6901 cccggcattg ctgtaaaatg taaatcagta ccgccgtaat atttcgaatt aaatggatat
     6961 tggtctggta ttactgtatt tacttttaca tcattgggag agccttcggg ttttacgacc
     7021 accacaagat tgtagggccc ctgtaccgga taacagtcca cgataccggt ttcgattttt
     7081 acacatcttt cgtttataaa gtctctgatg gcttttacgt tatttttcca tgtagtgaag
     7141 ttgcctcccc atctggctat ttgaccgggc atttcgggtt cgagctgtgc ttccatttca
     7201 ttgagcagat tggtcatata ctgacagctc aggaaagtat tgttaaggtc ggcatatcgg
     7261 ttgatgtaca gatcgaagaa agatttattg gtaaataatt tggtgagtac ctctgtatgt
     7321 ttttgtggat catagcttgg cccaacagtt tcaatatcgc acgggtctgc atctgcattt
     7381 acattaggta tgttggtata attgatataa tggccaaata ctgcatccat atcccagagt
     7441 gtataacgcc atttctgaaa attcccttcg ttatccatac ccgtccacca ggcggtattc
     7501 cacacgagcc agtctttgca cacggtatgc acattgataa tataataatc gatcaatgac
     7561 tgtaggttaa gcctgtctgc tacatactga aacttgacag gattggtcat atcatttgtc
     7621 aggatataat tgcgcagtgt aaaccaattg gcagttgtgc cgtattctgc ccatgtgctt
     7681 ccccaggttt tgatgaaatc aatttccttt tcatcaaggc cgtaataata atcagtataa
     7741 tctttatcat ctaccttttc tctgatatcg taaacacccc agtagttgcc attgacatac
     7801 aatacacaga aattagtaga acggacatca agatcaaggc ccgccctttg agacagttca
     7861 tgaacataag cgtctctcag atgtgctcct gttccgagtg caggataatt gtcattggct
     7921 gctgccttga ggataatacg ctgaaatttc ttgcgctttg tatttggaaa aatcttgtgt
     7981 tttatctcag aatcgttgcc aaactgatct cttgttacat agtcaatccc tcgttgtgca
     8041 taggcccacg aatcgttacc gtgtttgtta aattcgcctg tacatgcgtc aatgaatttg
     8101 ccattctggt cgaatagttc aaacgtgcct tgtggattat tttgagtacc acttaacaag
     8161 agtggcacat cgtcaccgga gattgaaact accatcatgg tatgcttgct ggcagcacct
     8221 atgaagtaag tgccaaaagc tgtgtgcgat gttagtatct tagggtcgga tgtataggtt
     8281 ctggccttaa tgattctggt agtacttacg tttatgggtg cactgtatgc cgttgaagcc
     8341 gcggttggtt cacttccgtc aagtgtatat ctgatggtgc tattgggctc ggtggtagtc
     8401 aaagtaacgc tttgtgtgcc tgcgtaatat ccgggctgga tattgaactg tggcgtaaga
     8461 gcatatctgg tcgtcgtaga attattggct gctccgggtg tgggattgtt ggttacagtc
     8521 catggtccgc catcaggcat acgtgcataa gcgtgatttt ttccgttggg tatgtctatt
     8581 tcatgaaaat ctataactgc gccactaggt gaggctagga caaacgcttc ctttgcatct
     8641 gtctgtgtca ccttaaaatt ggtatgcaga aaaccgccta tgacattgtc ttcgcctgag
     8701 gcaataatca atatcctgtc tccaggcgaa aggatatagc ctgccggtat ttgccatttg
     8761 gtaggtttgt caacacgatc gctcaggtag tatccctgta ggtttacagg tgctgcaccc
     8821 atattataca actctaccca atcttcataa tcattattag aacaaaggta attgtcataa
     8881 ttggcagctg aaaattcgtt tataataacc tgggcttttg tgtattgcaa gcctgtgatg
     8941 aaaacgaaaa ataataaaca atagcttgtc tgaaatattt tgtaaaacaa tttcatgttg
     9001 gttcaaaatt tttaaataag aatttaatat ctaagatact atatttcttc agattgtcca
     9061 ttctattgaa aatgttttta tcaggtatga agaatttctt aaattgaatg ctgtgtaaat
     9121 gtgttaactt tgcagaatat tttcaaattc aaccatcaaa acatgcgaga gaccaccgaa
     9181 ttaaagaaaa ttgctactca ggtacgaagg gatattgtcc gaatggtatt tgatgctcaa
     9241 tcggggcatc caggtggctc tttgggctgt gccgatttca tgactgccct ttatttttct
     9301 gtaatgaacc acaatccgga atttaaaatg aaaggcttgg gtgaggacat atttttcttg
     9361 tcgaatggcc acatttcgcc ggtcttttat agcgttctgg ctcgttcagg atattttcct
     9421 gtatcagaat tagccacttt cagaaaactc aacacacgac tacaggggca tcctaccaca
     9481 catgagggtc tgccgggcgt gcgggtagca tcaggttcac tcggtcaagg actttcggta
     9541 gcatgcggtg ctgcacttgc caagaaaatg gacaacgact catcaatagt ctatgccctt
     9601 accggtgacg gagagctaca ggaagggcag atatgggaag cagccatgtt tgccgcacac
     9661 cacagaatag ataacctaat cgtaacagtt gactggaacg gtcagcaaat agatggcagt
     9721 aacaatgaag tatgcagcct cgatgtattg cccgccaagt ggcacgcatt tgggtgggat
     9781 gtactccaga ccaatggcaa cgacatggac gtactcatcc atacactcca agaagccaag
     9841 aaagcatgca accgaggtcg tccaattgta atactcatga aaactgaaat gggctgtggt
     9901 gttgacttta tgatgggctc acacaaatgg catggtatag ctccaaatgc cgaacaactc
     9961 gaaaaagcac ttgcacaatt acctgaaaca ttaggcgatt attgattcac atttaatttc
    10021 atcaaaaaaa gatatgtcgg taaataattt aaccgtaaac ggcaaaaaag caacccggga
    10081 cggattcgga gacggactta ccgaactggg aagaaacaat cctgacatcg ttgccctttg
    10141 tgctgacctg ctgggctcac tcaagatgga tcagttcaaa aaggaaaacc ctgagagatt
    10201 cattcaggta ggaatagcag aagccaacat gatgggcata gctgccggac tcgctacttg
//
LOCUS       DAOQQY010000072         9992 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2488_length_9992_cov_6.393781, whole genome shotgun sequence.
ACCESSION   DAOQQY010000072 DAOQQY010000000
VERSION     DAOQQY010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9992
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2488_length_9992_cov_6.393781"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..1944
                     /locus_tag="PLE23_05205"
     CDS             214..1944
                     /locus_tag="PLE23_05205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMX82697.1"
                     /translation="MVRGYISLFIVLCNFGWHCSIAQKLDCYHELRLIDSLIEYTQHN
                     IAQQHITNLSNYLDKQGISKENQKIRLNLKYLEALNYDRQEANPEIVLVLLTKLSKET
                     KDIQLIDLSYKINLLTALSFEKAKNLAQCKKYLDEAHRLYQQQHLDSLYSTYCIRMGS
                     YYLFVPNYDSANYYGLLANKMARRYKNEKDLSDSHIILGSVAEEQKRIDDAILHQKQV
                     LAYTLKINHRSFAVTLYNNLALHYLDQNLYKEALQYNDSSMILRYALGLQEPKFTKTR
                     YKIFFQIGNKDSTNYYFNKYVHEEQTLLKENETAREKRLEAIHIIEKSKLLLDAKNKQ
                     IISIIFILGIILFGSLLLLHKNFQIDKKNKIIDRQLKELAVLLSQKQVLLAEFQHRVK
                     NNLQHVISILELQKESAQFNNIEELLRSNQNRVHSIALLHKKLDISENVDEVNMEKYF
                     TELAEMVKESYDRPGKTVKLFIECQVGVLPIDLALPLGLILTELVSNSMKYAFVNREI
                     GIIDFKFNSAFSFYEIWYNDNGEGFDFNQITQKGLGMELIKGLIEQIEGSITTPLTSG
                     FSIKISFPKN"
     gene            1951..2625
                     /locus_tag="PLE23_05210"
     CDS             1951..2625
                     /locus_tag="PLE23_05210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX82698.1"
                     /translation="MQKSILLVEDYFLNRRLVRKVLLANGYTLFEAKNAKEAIRILNE
                     NTIDLIILDINLGKGEQDGISIAEQINTSYHLPFLFMTAYDTQTILSRAVATRPYSYL
                     TKPFKNIDLIASIEVALKQFNKSADPKITVRDGKYSVEIFVKTIDYIESDGNYIILHT
                     DEKSYTLRATLKQVLEMLQNEEFVQVHRAYIVNKSKIEGTNKKYILIKGLEIPISKFF
                     LDDSVE"
     gene            complement(2672..3322)
                     /gene="tmk"
                     /locus_tag="PLE23_05215"
     CDS             complement(2672..3322)
                     /gene="tmk"
                     /locus_tag="PLE23_05215"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HMX82699.1"
                     /translation="MEAIKRKSLFIAFEGIDGSGKSTQLRRLTEFLQRKGYKVHTTAE
                     PTTRPIGRMIRDIFNHRMVADNRTIAGLFVADRLDHILNKDDGMLRLLDEGYIVITDR
                     YYMSSYAYQGAHVPLEWVIGANSLASELLRPDITIFIDMPVGKALDRVRKGRNTLELF
                     ESEDNIRQVTAMYEKVIKMIGSDENIVRINGDRSEDEVADEIETLVVAFLHDSLNS"
     gene            complement(3470..4480)
                     /gene="fbp"
                     /locus_tag="PLE23_05220"
     CDS             complement(3470..4480)
                     /gene="fbp"
                     /locus_tag="PLE23_05220"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMX82700.1"
                     /translation="MDINRIITLDEFIIRQQNEFAGATGELTGLLRDIGVAAKIVNRE
                     VNRAGLVNILGIEGTENQSGDVVQKLDLYANEKLIECLKNSGECCAIASEEIENFIEL
                     PGIHDKPAKYVVLFDPLDGSSNIDVNVSVGTIFSIFRRKSDFTGPSNIGDFLQTGLEQ
                     VAAGYVIYGTSTILVYTTGHGVNGFTLDPSIGEFCLSHRNIQIPKRGQYYSVNQGYYL
                     KFDLEMRRYIDHCSDLNLGLRYIGSMVSDVHRILFQGGIFLYPSTRKYPNGKLRLLYE
                     CNPMAFIVEQAGGKAINNHLNRILEIDPKELHQRSTIAIGSPDMVDEMKAFVERYSAV
                     TS"
     gene            4607..6391
                     /locus_tag="PLE23_05225"
     CDS             4607..6391
                     /locus_tag="PLE23_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX82701.1"
                     /translation="MQHLKVLVKYFVQYKWHLILGTIFVFLSNYAKVLQPQMIREALD
                     VVLDNIQQYHTATTPEQKNEIFNNVGAETLKFALLVLGLAVLMGVFMYMMRQTIIVMS
                     RLIEYDLHKEMFTHYQKLSTSFFKRNNTGDLMARITEDVSRVRMAIGPAILYGLNLFS
                     TFVLVIYAMLRVSPKLTLFVLLPLPLLSFIIFKVSTLINRRSEIIQQNLAILNSTAQE
                     SYSGIRVVKSFAQEVQMTDLFEEQTLNYRARTLDLIRVESVFFPSMALLIGLSTVLTV
                     YIGGLEVIKGNITPGNIAEFVIYVNLLTFPVTAIGWIASLIQRASASQKRINDFMNEK
                     PEIVNPVIDPVDIVGEIVFDHVSFQYPVTNIQALNNISFTLKPGERIAIVGKTGSGKT
                     TIAELLLRMYDIQSGSITIDGKEIRDLDLTNLRRRIGYVPQDGFLFSDTVAGNIAYGK
                     PNATGELIEEYARNASIHNEILSLPEGYQTIVGERGVTLSGGQKQRISIARALIKDPE
                     IVILDDCLSAVDADTEQKIMNYFKENLKGKTTIVITHRFSSMSFYDNILVLRDGEIAE
                     QGTHDELMKKGQLYKEMFERQNVEINNL"
     gene            6465..6851
                     /locus_tag="PLE23_05230"
     CDS             6465..6851
                     /locus_tag="PLE23_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HMX82702.1"
                     /translation="MENENFNQKSENVYSAKVRAGKRRTYFFDIRQTKGADYFLTLTE
                     STKRFNGDGYERHKLFLYKEDINRFVEGLEEAVKFFKEELLPDYDFDEYTKRQEEWEA
                     RKALEEQENPEPKNEKNIDSEEDMAW"
     gene            6948..7631
                     /locus_tag="PLE23_05235"
     CDS             6948..7631
                     /locus_tag="PLE23_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82703.1"
                     /translation="MIVRVLEELIAGFEKYLATQARTSDEPLLFYENTQHFQKNWAEP
                     VNALPDTLKRAVYNTQSKRFWYKPREILIAFASFNEEFTGLMLRDLFNETKALSGRIE
                     RFKYYCDTLLGELQEMDRKFPSDHFQTTEVIYFYLAMRFPEEYAVYQHKHFVKTLILL
                     SAKDIPVVFDPERFYKSAKIFNKYLKDNPLIDKHIEALLKGTNTFGLQNMLRVTYFCW
                     YCDNIHNNT"
     gene            7628..8722
                     /locus_tag="PLE23_05240"
     CDS             7628..8722
                     /locus_tag="PLE23_05240"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HMX82704.1"
                     /translation="MKKTLILILTLTVALSASAQYKYQPVFPGKKGDVLLAELVNTYK
                     PLTVLDYSTARDTLFFRVYREHDSLACVYSDMKLPLPDGVDPTTYVYLSGTPNGINTE
                     HAYPQSKGAVGAAKSDMNILLPSRSAVNEARGNLPYGESPDNQTQKWFYKTAALTSKP
                     LVNIDFYTELNASVFEPREAVKGDVARKIFYFYTMYKTEADAADKNFFNLQKNTLCQW
                     HLYDPVDSLEWERNKIIAKYQNNRENPYVLDCTLPYRTFCPDYQPSCDTTVATKTLLT
                     DGDVKIFPVPFRNNLQICTQTTLSSDIEVSVVDILGKIVFRQKSTKSDNCLLLDAPEI
                     TALAPGFYFIHILQNKAAGMNYIIKPVIKE"
     gene            8791..>9992
                     /locus_tag="PLE23_05245"
     CDS             8791..>9992
                     /locus_tag="PLE23_05245"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HMX82705.1"
                     /translation="MERFIGLIGIVLILGIAFLMSNNRKRINYRLVLSGLALQLGLAL
                     FILKVPIGKTIFAGFASIVTKILSYADRGADFVFGALVNKEYMEKAFGAGHDFVFFFK
                     IIPTIIFVAVLVSIAYYLGIMQRIVAVIARFVHWLMGVSGSEALSNVASAFVGQVEAQ
                     IMIKPYLKGMTNSELLASMAGSMACIAGGVMAVYIALGVPAEYLLAASIMAAPGALVI
                     SKIVFPETEQSETKGHVKLEVNKTSSNFVDAISHGASDGLKVGLNVIAMLIGFIALIA
                     MTDAVLGFLGRNIYQYTGFTLDIIGIDFQQLSLKTILGAIFSVFAWAMGVPMQDVYTA
                     GSLMGTKMVVNEFVAYLDMSAMLKSGALDPKTITIVSFALCGFANFSSIAIQVGGIGE
                     LAPSRRTD"
BASE COUNT         3099 a         2036 c         1963 g         2894 t
ORIGIN      
        1 tatcaaatcc ccctttttga gcatccaaaa tgatccctaa tgaatttttt gggataaaat
       61 gtaaaattta gttttatttc caaaagccct ttcaaagttt gcgctttatc agggctttta
      121 ttcagcctga aaataatttt ataatttatt attgaataat ctgttcgtat ggaataatat
      181 tctaattttg actgaattgc tatttagttt tgtatggtgc gaggatatat ttcattgttt
      241 attgtgcttt gtaattttgg ctggcattgt tctattgcac aaaaactgga ctgctaccat
      301 gaattaagac tcatcgacag cctgatagaa tacactcaac acaatattgc acaacagcat
      361 atcacaaatc tcagcaacta tttggataaa caaggcatat caaaggaaaa ccaaaaaatc
      421 cggctgaatt taaaatacct tgaagcactc aattatgacc ggcaggaagc aaatcccgaa
      481 attgttctag tgcttttgac aaaactgagc aaagaaacca aagacataca attaatagat
      541 ttgtcatata agataaattt gttgacagct ttaagttttg agaaagctaa aaacttagct
      601 caatgtaaaa aatatcttga tgaggcacac aggctttatc agcaacagca tctcgattct
      661 ctctacagta catattgtat aagaatgggc agctattact tatttgttcc aaattatgac
      721 tcggcaaact attatgggtt gctggcaaat aaaatggcac gccgctataa aaatgaaaaa
      781 gatctgtcag attctcatat tatattaggc tccgtggctg aagaacaaaa acggatagat
      841 gatgctattc tacaccaaaa acaggttttg gcttacactc tcaagattaa tcacagatca
      901 tttgcggtca ctttgtataa taatctggca ttgcattatc ttgatcagaa tttatacaag
      961 gaggcattgc aatataatga ttcctccatg attttaagat atgctttagg tctgcaggaa
     1021 ccaaagttta ccaaaaccag gtacaagatt tttttccaaa taggaaataa agattccacc
     1081 aattactact ttaataaata tgtgcatgaa gaacaaacat tactaaaaga gaatgaaaca
     1141 gccagagaaa aacgactgga agcaatccac ataatcgaga aaagcaaact attgttagat
     1201 gcaaaaaaca agcaaattat aagcatcatt ttcatattag gtatcatttt gtttggctct
     1261 ttgttgttac tccacaagaa ttttcaaata gacaaaaaga ataaaattat tgaccggcaa
     1321 ctaaaggaat tagcagtatt gctcagccaa aagcaggtgt tgcttgctga gtttcagcac
     1381 cgagtgaaaa ataacctaca acatgtcatt tcgatattag aactccagaa agaaagtgcc
     1441 cagtttaaca atattgaaga actgctgcga agtaaccaga atcgagttca ttccattgcg
     1501 ctacttcata aaaaattgga tatatcagaa aatgttgatg aagtaaatat ggaaaaatac
     1561 ttcacagagt tggctgaaat ggtcaaggaa tcttatgaca gacccggaaa aaccgtcaaa
     1621 ctttttatcg aatgtcaagt gggtgtatta ccaattgatt tggctttacc tcttgggcta
     1681 attttgacag aactagtcag taacagtatg aaatacgctt tcgtaaaccg cgagatcgga
     1741 ataattgact ttaaattcaa ttctgcattt tctttttatg agatttggta caatgacaat
     1801 ggtgaaggat ttgacttcaa ccaaataaca caaaaaggac tcggaatgga attgataaaa
     1861 ggcttgatag agcagatcga aggctctata actacacctc tcacttctgg attcagtatt
     1921 aaaatttcat ttccaaaaaa ttaagaaaag atgcaaaaat ctatactgtt ggtagaagac
     1981 tattttttga accgaagatt ggtacgaaag gtactgttgg ctaacggcta tactcttttc
     2041 gaagcgaaga atgcaaagga agctatccga atactgaacg aaaatactat tgatctaatc
     2101 atattagaca ttaacttggg taaaggggag caagatggta tcagtatagc agaacaaatc
     2161 aacaccagct atcatcttcc gttcttgttc atgacggcct atgataccca gaccatccta
     2221 agtagagctg ttgctaccag accatattct tacctcacca aaccatttaa gaacattgat
     2281 ctgatagcct ctatcgaagt tgcacttaaa caattcaata aaagtgccga tccaaaaatt
     2341 actgtaagag atggtaaata ttcagttgaa atttttgtaa aaactattga ctacatagaa
     2401 tcagacggga attatatcat cctacatacc gacgaaaagt catatacgct tcgcgccaca
     2461 cttaaacaag tacttgaaat gcttcaaaat gaagaattcg ttcaggtaca tcgtgcatat
     2521 atcgtcaaca aatcaaaaat tgaaggtacg aataaaaaat acatacttat taaaggtctc
     2581 gaaataccaa tttcaaaatt tttccttgat gatagtgtgg agtgaataga agtagttgaa
     2641 caaacaaaaa ttggcaatag ctggtcaatc ttcaactatt gagagaatca tgcaaaaaag
     2701 ctacgactaa agtttctatc tcgtctgcca cctcatcttc acttctgtcg ccattgatgc
     2761 gtacaatatt ttcatcgctg ccaatcatct tgattacctt ttcgtacatg gctgttacct
     2821 gtctgatatt gtcttcagat tcgaacagtt cgagcgtatt ccgtcccttg cgcacccgat
     2881 cgagtgcttt tccaacaggc atatcgataa aaatggtaat atccggcctc agtagttcgg
     2941 atgcaagcga attggcacct atcacccatt cgagcggtac atgtgctcct tggtaggcat
     3001 acgacgacat atagtaacgg tcggtgatga cgatatagcc ctcatccaag agcctgagca
     3061 ttccgtcatc cttattaagt atatggtcaa gcctgtctgc tacgaataat ccggctatgg
     3121 tgcggttgtc agctaccata cggtggttaa atatgtcacg aatcatcctg ccgataggcc
     3181 tggtggtagg ttcggcagta gtgtgtacct tatagccttt cctttgcaaa aattcggtaa
     3241 gtcgccttag ctgcgtgctt ttaccacttc cgtctattcc ttcgaatgct ataaacaatg
     3301 attttctctt gattgcttcc atttatcatc aattaccggt acatgcaaat gtgtttcccc
     3361 aacatattta acgtggtcgt accggctgtt atggtatgag tgaccgtcta tttttttaat
     3421 attatttttt tggctccgca aatgttatac aagaatctca atctggtttt tagcttgtaa
     3481 cagcactgta tctttctacg aatgccttca tttcatctac catatcaggc gaaccgatgg
     3541 caatagtaga gcgttggtgt aactctttag ggtcaatctc cagaatgcgg ttgaggtgat
     3601 tgttgatagc cttgccacct gcctgctcaa caatgaaggc catagggttg cattcataca
     3661 gcaacctcag cttgccattt ggatactttc tggtactcgg atacagaaat attccaccct
     3721 ggaacaatat tctgtggaca tccgaaacca tagacccaat gtagcgaagg cccagattga
     3781 gatcggagca atggtcaatg taacgacgca tttccagatc gaatttgagg taatatccct
     3841 gattgacgga gtaatattgg ccgcgcttag gtatctgtat attgcggtgc gacaggcaga
     3901 attcgccaat ggaagggtcg agtgtaaatc cgttgacacc atgcccggta gtataaacca
     3961 gaatggtcga agtaccgtat atcacatagc ctgcagctac ctgctcgagg ccggtctgta
     4021 gaaaatcacc tatgttgctc ggaccggtga agtcactttt ccttctgaaa atcgaaaaaa
     4081 tggtgcctac acttacattt acatcaatat tggaagaacc atcaagtgga tcgaagagta
     4141 cgacatattt tgcaggtttg tcgtgtatgc ccggcagttc tatgaaattc tctatttctt
     4201 cagaagcaat ggcacaacac tcgccactgt ttttgagaca ttcgatgagt ttttcgttgg
     4261 catacaagtc aagtttttgt actacatcgc ccgactgatt ctcggtgcct tcgataccaa
     4321 gaatgttgac aagtccggcg cggttcactt cgcggtttac tattttggca gctacgccaa
     4381 tgtcacggag aagtccggta agctcaccgg tagcacctgc aaactcgttt tgttgtctga
     4441 taataaattc gtcaagcgtt ataatcctgt tgatatccat tatgtccgat gattttgttg
     4501 gcccaaagat aagtaaaagc ctccacttta cagttttttt gtcaaatctg ttccgttttt
     4561 tcaaccattg ggcagaaatg attgtttgaa tcgatatttt tgcatcatgc agcatctaaa
     4621 agtacttgtc aagtatttcg ttcagtacaa atggcacctt atattgggta ccatttttgt
     4681 cttcttatct aactatgcca aggtgctaca gccgcaaatg atccgcgaag cactcgatgt
     4741 agtacttgat aatattcaac aataccatac tgctactaca ccggaacaaa aaaacgaaat
     4801 ttttaacaac gtaggtgctg agaccctaaa atttgcactc ttggtactcg gtttggctgt
     4861 attgatgggt gtctttatgt acatgatgcg ccagaccatc attgtcatgt cgagactcat
     4921 agaatatgac ctccataaag agatgttcac tcattatcaa aagctttcaa cttcattttt
     4981 caagagaaat aatacaggcg atctgatggc tcgcatcacc gaagatgtat cgcgcgtgcg
     5041 aatggctatt ggtccagcca tactatatgg tcttaaccta ttttctacat tcgtattggt
     5101 aatctatgcg atgttgagag taagccccaa gcttaccttg ttcgtgttat tgccattacc
     5161 tctcctatcg ttcatcatat tcaaagtaag tacgctcata aaccgcagaa gtgaaatcat
     5221 acaacaaaac ctggcaatac tcaacagtac agcccaggaa tcgtactctg gcatccgtgt
     5281 tgtcaaatca ttcgcacagg aagtacagat gaccgatctg tttgaagaac aaacactcaa
     5341 ttaccgggca cgtacactcg acctcatcag agtcgaatcg gtattctttc cttctatggc
     5401 attgctcata gggctgagca ctgtactgac tgtatatata ggtggactcg aagtcatcaa
     5461 aggaaatatt acgccgggca atattgccga gttcgtcatt tatgtcaatc tgctgacttt
     5521 tccggtgaca gccattggct ggattgcatc cttgatacag cgagcatctg cctcacaaaa
     5581 gcgaatcaat gatttcatga atgaaaaacc agagatcgtc aatccagtca ttgacccggt
     5641 agatattgtt ggtgaaattg tcttcgacca tgtcagcttt cagtatccgg tgaccaatat
     5701 tcaggcgttg aacaatataa gttttacatt aaaacctggc gaacgcattg caatagtagg
     5761 taaaacaggc agcggaaaaa ccaccatagc cgaactcctt ctgagaatgt atgatataca
     5821 atcgggcagc atcaccattg acggtaaaga aattcgcgac ctcgacctca ccaatctgag
     5881 aagaagaatc ggttacgtac cacaggacgg attcctgttc tctgatactg tagccggcaa
     5941 catagcatac ggcaaaccaa atgccaccgg cgaactcata gaagaatacg caagaaatgc
     6001 ttccatccac aacgaaatat tatcattgcc cgaagggtac caaacaatag taggcgaacg
     6061 tggtgtaacc ttgtcgggtg gccagaaaca gcgtatttct atagcgcgcg cccttatcaa
     6121 agaccccgag atcgtgattc tcgacgattg cctctcagca gtagatgccg ataccgaaca
     6181 gaagattatg aactatttta aagaaaacct gaaaggcaaa actaccatcg tcatcaccca
     6241 cagattctct tccatgtcct tttatgacaa tattctagta ttgcgcgatg gagaaatagc
     6301 agaacaagga acacacgatg aactgatgaa aaagggccaa ctttacaaag aaatgttcga
     6361 aaggcaaaat gtcgaaataa ataatttgta agctattgtt ttaatatata aaatcatttg
     6421 tataaatttg tacagaagat tttataccaa taaaacacat tagggtggaa aacgaaaatt
     6481 ttaatcagaa atctgaaaat gtttactctg caaaagtcag agcgggcaaa cggcgcacat
     6541 atttttttga tatccgtcag acgaaaggtg cagattattt tttaacgctt actgagagca
     6601 ccaaacgctt taacggcgat ggctatgaaa ggcacaaact gttcctttac aaagaagaca
     6661 tcaatcgctt tgtcgaaggc ctcgaagagg ctgtgaagtt ttttaaggaa gaattgctcc
     6721 cggactacga ctttgacgaa tacaccaaac gccaagaaga atgggaagca cgcaaagctc
     6781 ttgaagaaca agaaaaccct gaaccaaaaa acgaaaaaaa tattgattct gaagaagata
     6841 tggcctggta agaatttccg gacgtatcct tttgaacttg tcaacacacg tcgctgttgt
     6901 gtgttgtgtg ttatctgaat gtatatgatg aaaaaagtaa gcgggaaatg attgtaaggg
     6961 ttttggaaga attgatagcc ggatttgaaa aatatctggc cacccaggcc cgcacttccg
     7021 acgagccact gcttttttat gaaaacaccc aacactttca aaaaaactgg gccgaacctg
     7081 ttaatgcact acctgacaca ctgaaacgtg cagtttacaa cacacagtca aagaggtttt
     7141 ggtacaaacc tcgtgagata ttgattgctt tcgcatcctt taatgaagaa tttacaggcc
     7201 tgatgctacg cgatttattt aatgaaacca aagcattatc cggccggatc gaaaggttta
     7261 aatattactg tgatacactg cttggtgaac tacaggaaat ggatagaaaa tttccttcag
     7321 accatttcca aactactgaa gtcatttatt tttatcttgc catgcgcttt cctgaggaat
     7381 atgctgtgta ccagcacaag cattttgtta agacactcat attgctgagt gccaaagata
     7441 ttccggttgt atttgatcct gaaagattct ataaatctgc aaaaattttt aataaatatc
     7501 tgaaagataa ccctttaata gataagcaca tagaagcatt actcaaaggc accaatacct
     7561 ttggcttgca aaatatgctc agagtcacgt acttttgttg gtattgtgac aatatacata
     7621 ataacacatg aaaaaaactc taatactgat acttactctg acagtagcgc tttcggcctc
     7681 agcccaatac aaataccagc cggtttttcc gggaaagaaa ggtgatgtcc ttctggcaga
     7741 attggtcaat acttacaaac cactaactgt actcgactat agcaccgcac gagataccct
     7801 tttcttcaga gtatatcgcg aacatgacag tctggcttgt gtatattctg atatgaaact
     7861 tccactaccg gatggtgttg acccaactac ctatgtttac ctgagtggga cacccaatgg
     7921 catcaatacc gaacatgcct atcctcaaag taaaggtgca gtaggagcgg caaaaagcga
     7981 tatgaacatc ctcttaccat ccagaagtgc cgttaacgaa gcaagaggaa atctacctta
     8041 cggagaaagc ccggataacc aaacccagaa atggttttat aaaacagcag cacttacttc
     8101 aaaaccactt gtcaatatag atttttacac tgaactcaat gcttcggttt ttgaacctcg
     8161 tgaggctgta aaaggtgatg tggccaggaa aattttctac ttctacacga tgtacaagac
     8221 agaagcagat gcagccgata aaaatttctt caatctgcaa aaaaatacgc tctgccagtg
     8281 gcatctgtac gatccggttg attctctcga gtgggaacgc aacaagatca tagcaaaata
     8341 ccagaataac cgtgaaaatc cctacgtact cgactgtacc ttgccgtacc gcaccttctg
     8401 cccggactac caaccttctt gtgatactac agtagctaca aaaactttgc ttacagatgg
     8461 agatgtcaag atatttccgg ttcctttcag gaataatcta cagatctgta ctcagactac
     8521 tttgtcatct gacatcgaag tgtctgtggt cgatatattg ggtaaaattg ttttcaggca
     8581 gaaatcgaca aaatccgaca attgtctttt gcttgatgca cctgaaatta ctgctttggc
     8641 tcccggattt tatttcattc acatcttaca gaataaagct gcaggcatga attatatcat
     8701 taaaccggtg atcaaagaat aatttttttt ctttgcattg cagttttatt tcagaatatt
     8761 aatattttca accctttaaa tgaaaatact atggagagat tcatcgggtt gataggaata
     8821 gtgctgatac tcggcattgc attcctgatg tcaaataaca gaaaacggat caattacaga
     8881 cttgtattat ccggtttggc cttgcaattg ggtttagcat tgtttatttt aaaggtaccc
     8941 attggtaaaa caatttttgc cggatttgca agtatagtca ccaagatact ttcctacgca
     9001 gaccggggag cagacttcgt attcggggcg cttgttaata aagaatacat ggagaaggca
     9061 ttcggtgccg ggcatgattt tgttttcttc ttcaagatta ttcctaccat tatttttgtt
     9121 gctgtattgg ttagtattgc gtattatctc ggtattatgc agcgtattgt ggcggtcatt
     9181 gctcgttttg tacactggct catgggcgtg agcgggtccg aagcattgtc caatgtggct
     9241 tcggcatttg ttgggcaagt agaagcccaa atcatgatta aaccctacct taagggaatg
     9301 accaactccg aactacttgc ttcaatggca ggaagtatgg cttgtattgc cggaggagta
     9361 atggcagtat atattgcact cggtgtaccg gccgaatacc tccttgctgc ttccattatg
     9421 gccgctccgg gtgctctggt gatttcgaaa attgttttcc ccgaaacaga acaatccgaa
     9481 accaaaggcc atgtgaagct cgaagtaaac aaaaccagct ccaacttcgt ggatgccata
     9541 tcgcatggtg ccagcgacgg gctcaaggtt ggtctcaacg tcatcgccat gttgattggg
     9601 ttcatagcat tgatagccat gacggatgct gttttaggct tccttggcag gaatatttat
     9661 caatatacag gattcacact cgacatcatc ggtatcgatt ttcaacagct tagtcttaag
     9721 acgatactcg gtgccatatt ttctgtattt gcctgggcta tgggtgtgcc tatgcaagat
     9781 gtctatactg ccggttcgct tatgggtacc aagatggttg tcaatgaatt tgtagcttat
     9841 cttgatatgt cagcaatgtt aaagtcaggt gcgcttgatc ctaagactat taccatcgtt
     9901 agttttgcat tgtgcggctt tgccaatttc agttcgatag ctatccaggt aggtggtatc
     9961 ggtgagctgg caccatcacg cagaacggat at
//
LOCUS       DAOQQY010000073         9989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2491_length_9989_cov_5.296356, whole genome shotgun sequence.
ACCESSION   DAOQQY010000073 DAOQQY010000000
VERSION     DAOQQY010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2491_length_9989_cov_5.296356"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="PLE23_05250"
     CDS             complement(<1..2071)
                     /locus_tag="PLE23_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HMX82706.1"
                     /translation="MLFIPSDSVEKLEFDKIRQLVALEAYGQPAVDRIYSIVPSSDFK
                     KIMIQLHQSDECKASIVTNDKVPMTAYEDISACLQPLHIVDYVLDIEDILRIRNLYLL
                     GYDILKYFDGSRKIAYPHFSQLVENVDNDDSILIYFNKIFTPDGQIRNDASETLVSIR
                     KKMATRQAVLEKQFRFLLNSYKSKEYLAESSESIRNGRKVLCVLAEHKRKIRGIIHDE
                     SATGKTAYIEPEEIIEINNDIFDLEQEERREIYRILKALCAFLSDKTALLQNYQELIT
                     EIDIINAKARFALKYNGNKPRLLNKPYLSIIEGYHTLLYLKNKELGKPTIPFTLQLKN
                     DNRIVIISGPNAGGKSITLKAVGLLQLMVQSGMLVPVDKNSEFGIFQSLFTDIGDQQS
                     IEDDLSTYSSRLRFIKEFLDVANENSLAIIDEFGSGTDPKIGGAIAEAALSEINKQKS
                     FGVITTHYSNLKLFAYKTKGIINASMHFDKERLKPSFELLVGKPGSSYAYEIAQKTGF
                     TKELLDYARHKTGKNEKAIDEILADLQAELKVLQDKISEVKEREIFVDKLIKNYNDLQ
                     KDLEFRRKKFKLENKESNLQKIAHDSKQVEKVLREIKEMQNVKKAEILATELKEAKKS
                     LEKDILILEQEVVKEIPLTALKKKIEEGDYVRIKTGGAIGKLLSLGKEKALVEMGGMT
                     LTVKAKDL"
     gene            2166..3248
                     /gene="aroC"
                     /locus_tag="PLE23_05255"
     CDS             2166..3248
                     /gene="aroC"
                     /locus_tag="PLE23_05255"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMX82707.1"
                     /translation="MAGNSFGKVFRITSFGESHGPYVGVCIDGCPAGLKIDIDFIRKK
                     LQQRRPGQSGLTTSRTEREDFQIISGVFEGMSLGTPITILIPNDDTITKDYDQLKNIY
                     RPSHADYTYDVKYGIRDHRGGGRSSARETVARVAAGAIAQMLLHTAGVVVFSYTRKIG
                     KLELAVEPDSIGLKKIYTHDTRCPDLEMADKMSEYIREIKAQGDTVGGIIETIVTGMP
                     AGLGEPVFDKLNADLGKGILSINACKGIEFGSGFKAAGFKGSEHNDIFINQDGDIKTT
                     TNHSGGIQGGISNGMNLVFRAVFKPVSTIQKTQATLNINKEATDFIAGGRHDPCVIPR
                     AVPIVEAMTSIVLADHLLRNKLSRIS"
     gene            3341..5935
                     /locus_tag="PLE23_05260"
     CDS             3341..5935
                     /locus_tag="PLE23_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82708.1"
                     /translation="MQKKIVLISAYLIITVAGIFTIHSLQIKHQQAVYRLPFMPKPTE
                     TPGFVKEEKDSKTKREKWFEAMHRAAPGVDWRAIELQNQKEKAKSRAILRKQNVNRST
                     IEYLADSTLQGQWYERGAKNQAGSMVYTDYDPETDYLYSVAAGGSIFKGTSEGADWHA
                     LNQEYKFDDRFIRIYNGPADKRITCVLNKQMVNSDDDGQTWSAASGQNNASEIDRVKD
                     FLILKDADNTAMYLAREKASGNAYTIRLYISKDKGSSYTLIKDFGNRPLNELTITNPG
                     NTNDVYLIEKISQLATAIHKWDFVNNDFVKLNQANFSIGGSSSFKVTSAWMTDHVRFY
                     LYNSTEFVHYTDDYGVTWVNKGKLAASPWGVGIFAFPSDPKKLLMGEVECYRSDFEGS
                     IWQKINGWGEYYSKVKTKLHADMMHFNEFQKSDGTNFGVICCHGGIYTTKDYGKTVNN
                     ITLEHLNNAQYYDVVTQPGTFDYIYAGAQDQGFQRAQSNIDDKSAADFVQVISGDYGH
                     SCFTKGGSGLWTVYPGGSISFYNNPKSQGGPTAYYELDSDNETVWIPPIIPGPNNNAH
                     EVFLAGGNKDGGAGSHLIKLTYKQGTIDATQFEFDFKANSGSEISAMAISPFDSTIYY
                     VATNNGKFYISPDAGASFFESSPKVPGSHYLYGACIYPSKLNPDVIYLSGSGYSNSGV
                     LVSTDGGFSFNPLGEGLPKTFVFNVVANEDESLLFAATEAGPYVYSVAKQKWFDMSGI
                     AAPTQTFWSVEYIPGHKVVRFGTYGRGIWDFRIDDLTTSVAQYNSDNSLLMTIAPNPA
                     RNFLNITLPQDETFDVEIFNMNGQVVLKPGKINLVKNINVQALLPGNYIIKASSKKRN
                     ISKIFVKQ"
     gene            complement(6008..6436)
                     /locus_tag="PLE23_05265"
     CDS             complement(6008..6436)
                     /locus_tag="PLE23_05265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX82709.1"
                     /translation="MIASIEFATPEYDEAVSLRYKILRKPLDLEFSEEQLMEEFDQFH
                     FGYYNLQGKLIACLSYQLKEDGVLKMRQVAVDDSQQGRGIGTRMVKETENWAQSSGYN
                     KIILHARDTAVKFYKKLNYSIKGKDFTEVGIKHFLMEKTL"
     gene            complement(6444..7295)
                     /locus_tag="PLE23_05270"
     CDS             complement(6444..7295)
                     /locus_tag="PLE23_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HMX82710.1"
                     /translation="MIKLIECPRDAMQGLHDFVETKDKIRYIQALLEVGYDTIDFGSF
                     VSPKAIPQMRDTAEVLGSLDLSKTRSKLLAIIANTRGAEDACRFEQIDYLGYPFSISE
                     TFQLRNTNATIEESLVRVEEIQELCLKYNKKPVIYISMGFGNPYGDVWNVEIVEKWVS
                     ALRDMGITILSLSDTIGVSNPESIDYLFSKLIPNYPEVEFGAHLHSEPHNWFEKIDAA
                     YTNGCTRFDSAIKGFGGCPMAKDDLTGNMPTENLIQYFTDKRVELNLNMEAFYKSMEI
                     ALQIFPK"
     gene            7403..7618
                     /locus_tag="PLE23_05275"
     CDS             7403..7618
                     /locus_tag="PLE23_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82711.1"
                     /translation="MKGILSLVGFLMFSTGIVGLVLSLVGVRLSVLSFIESATPMLGF
                     LFKLFLCVAGIILVIVARTDWKKENQA"
     gene            complement(7615..>9989)
                     /locus_tag="PLE23_05280"
     CDS             complement(7615..>9989)
                     /locus_tag="PLE23_05280"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HMX82712.1"
                     /translation="ASIYIAGSTSGVNTNVNGYYSLNLAKGESEVIFQYIGYKTVSKK
                     INYTGVPLKLNITLEAQSLEIAEVEINASGEDPAYAIIRNAIANREYFKNHIKSYSCD
                     VYIKGNQKIVKIPQKLMGEDLGNLGGILDTNRQGIIYLSESLSKFYFQAPDKTREEII
                     SSKVSGDSRGFSFNRATAIRFSFYDVAVDFGRQLISPIAPEALLFYKYRLEGSFFDSD
                     GREVNKIKVIPRNESGPVFRGDIFIYENTWNIHSCHLGVTGKSINQEILDTMWLSQQY
                     VPGNEQGQWVLFSQNIEFGLGLLGIKIKGFFTAVFGNYSLNNNFDNTIFSKELVKIEA
                     GSNKKSDIYWDTLRPIPLLAEESRDFHRKDSLEVIWNSKAFRDSIDRKNNKFTSNNLT
                     FGYTYHNSFRRWSLNVGSPLTTIEFNPVQGFYSALNVKYRKWKDDNNTSWFSLGPEVS
                     YGFSDKKWRGALEFERLFNKLHYNKLALKLGKITAQYNETNPISATVNGLYNLYGKLN
                     YMKLYDKTFGKVSYEQRLWHTLKLNLESEYAIRSGLDNSSNYSFRKKSDVYSPNFPGD
                     TVGILPKHHYIGMNASITWFPGIRYVTYPDQVQYFNQSLPVITFRYYLKKVLSYTDGS
                     NGHYTIQQPEITVKYDFYPRFLGTTNISITGGTSIYSGNKAEIIDYKHFKGNQTFIAG
                     TGSEGFSLLPYYDYSTLGTYAEVHARHNFGGFFLSKIPLIKELKFEENITTNMLFQEN
                     GFRYTEISAGLSNIGWSIFRLFRVDYTWVFLNGKRHDSGITVGIKLPIAD"
BASE COUNT         2996 a         1913 c         1922 g         3158 t
ORIGIN      
        1 ccagatcttt tgctttgaca gtgagtgtca taccgcccat ctctaccagc gccttttctt
       61 taccaagtga taataatttt cctattgcac caccggtttt aattcttaca taatctcctt
      121 cctctatttt cttcttaagc gcagtcagag gtatttcttt taccacctct tgttcaagta
      181 tcagaatatc tttttctagc gattttttgg cttccttaag ttctgttgca agtatctcag
      241 cttttttgac attttgcatt tcctttattt cccgcaatac tttttcaact tgcttgctat
      301 catgtgcgat tttttgaaga ttgctttcct tattttcaag cttgaatttt tttcgccgga
      361 attccaaatc tttttgaaga tcattgtagt tctttatcag tttgtcaacg aatatttctc
      421 tttcttttac ttcagaaatt ttatcctgca aaaccttcag ttcagcttgt aaatctgcca
      481 gaatttcatc tatagccttt tcattcttgc cggttttgtg acgagcataa tcgagtaatt
      541 ctttcgtaaa tccggttttt tgagctattt cgtaggcata cgagctacct ggtttgccca
      601 ccaataactc aaatgacggt ttgagacgtt ctttatcaaa gtgcatacta gcattgatga
      661 ttccctttgt tttgtaagca aataatttca aattgctgta atgtgtggta ataaccccga
      721 aggatttttg cttattgatt tcgctcaatg ctgcttctgc tatcgcgccc cctatttttg
      781 gatctgtacc ggaaccaaat tcgtcaatta tggcaagaga attttcattt gctacgtcca
      841 gaaattcttt tatgaatcta agtctgctgc tataggttga taaatcatcc tcaatactct
      901 gctggtctcc aatatccgta aacagactct gaaaaatacc aaactcagaa ttcttatcga
      961 ccggaacaag catgccggat tgtaccatca actgtaacaa accaaccgct ttgagtgtaa
     1021 tagatttacc tccggcattt ggtccgctta taatgacaat acggttatca ttttttagct
     1081 gcaaagtaaa tggtatggta ggtttaccaa gctctttgtt ttttaagtaa aggagcgtgt
     1141 gataaccttc gatgatgctt aaatatggtt tgttgagtaa tctaggttta ttcccattgt
     1201 attttaatgc aaatctggcc tttgcattga ttatatcaat ttctgtgatt agctcttggt
     1261 aattctgaag taatgctgtc ttgtctgata aaaatgcaca aagtgctttg agtattctgt
     1321 aaatctcacg cctttcttct tgttcaagat cgaaaatatc gttgtttatt tcaatgattt
     1381 cttcgggctc tatgtacgct gttttaccgg tagcggattc atcatggata ataccacgta
     1441 ttttgcgttt atgttcagct agtacacaga gtactttcct cccgttacgg atactttctg
     1501 aagattctgc caagtattct ttagatttat acgaattgag taaaaacctg aactgtttct
     1561 ctaacactgc ctgccgggta gccatttttt ttctgatgct taccaaggtt tctgaagcat
     1621 cattacgtat ctgaccgtca ggtgtaaata ttttattgaa ataaatcaat atactatcat
     1681 cattatcaac attttctaca agttgagaaa aatgtggata ggctattttt ctgctaccat
     1741 caaaatattt tagtatgtca taaccgagta ggtaaagatt gcggatacga aggatatctt
     1801 caatatcgag tacataatct acaatatgca gaggttgaag acaagctgaa atgtcttcgt
     1861 aggcagtcat cggtactttg tcattggtga ctattgaagc cttacattca tcagattggt
     1921 gtaattgtat catgattttt ttaaaatcag acgatggcac aatactataa atacgatcta
     1981 cagcaggttg gccgtatgcc tccaaagcta cgagttgtcg aattttgtca aactcaagtt
     2041 tttcgaccga atcagatggg atgaaaagca tatttttaaa ttgaaaccaa aattagtgta
     2101 tgatgcgtgt ttaataacgg tttttgttga taattggttt aattttgata taaaatacat
     2161 agaaaatggc aggtaatagc ttcgggaaag tattcagaat tacgagtttt ggtgaatcgc
     2221 acgggcctta cgttggagtt tgtatagatg gttgccctgc cggattgaag attgacattg
     2281 attttattcg taagaaacta caacagcgac gtccaggcca atcaggatta accacttccc
     2341 ggacagaaag agaggatttt cagattatct caggtgtttt tgaaggaatg tcattgggca
     2401 cacctattac tattcttata ccgaatgatg ataccattac gaaagattac gaccaactga
     2461 aaaacatata cagaccttcg catgctgatt atacgtacga tgttaaatac ggcattagag
     2521 accatagagg aggcggtcga tcatctgccc gcgaaactgt tgcacgtgta gcagccggcg
     2581 ccatcgctca aatgttatta catactgccg gtgttgtagt attcagctac acaagaaaga
     2641 ttggcaaatt agaacttgca gtagaacctg attctattgg tttgaaaaaa atttatacac
     2701 atgacacacg gtgccctgat ctggaaatgg ctgataaaat gtctgagtac atccgggaga
     2761 ttaaagcaca aggcgatacc gtcggaggaa taatagaaac gattgtcact ggtatgcccg
     2821 caggcttagg tgaacctgtt tttgacaaat tgaatgcaga cctgggcaaa ggcatattaa
     2881 gcataaatgc ttgtaaaggc atagaatttg gctccggatt caaagcagcc ggtttcaagg
     2941 gctctgagca caatgacatt ttcataaacc aagacgggga tattaaaacc actaccaatc
     3001 attcgggtgg catacaaggc ggtatttcga acggtatgaa tcttgtattc cgggcagtat
     3061 ttaaacctgt atcaaccatt caaaaaactc aagcaacgct aaacatcaac aaagaagcta
     3121 cggatttcat agccggagga cgtcatgatc cttgtgtaat accaagggct gttccgattg
     3181 tagaagcaat gacttcaatc gtgttggcag atcatttatt gagaaataag ctgagtagaa
     3241 tatcataact ctgtaaaaaa aataggaatt tttatgtatt acaattgaaa attgcgtata
     3301 aatgtcttaa atttgacaaa ttaaatcatt caagaatagt atgcaaaaaa aaatagttct
     3361 tatttctgcc taccttatta taacggtagc cggaatattc actatccatt ccttacaaat
     3421 caaacatcaa caggcagttt accgtttgcc tttcatgcca aaaccgaccg agactccggg
     3481 tttcgtgaaa gaagaaaaag acagtaaaac gaaacgcgag aaatggtttg aagctatgca
     3541 cagagcagct ccgggtgttg attggcgagc cattgaactg caaaaccaaa aagaaaaagc
     3601 aaagtcaagg gctattttaa gaaagcaaaa tgtaaaccgt tctacaatag aatatttggc
     3661 tgattctact ctgcaaggac aatggtacga acgtggtgct aaaaaccagg ccggcagtat
     3721 ggtttatact gattatgatc cggaaaccga ctatctatat tctgtggcag caggaggttc
     3781 aatattcaag ggaacatctg aaggtgctga ctggcacgcc ctcaatcagg aatacaaatt
     3841 tgacgataga tttatcagaa tatacaacgg cccggcagac aagcgaataa cttgtgtact
     3901 taacaagcaa atggtaaact cagatgatga cggacaaacc tggtctgcag catccggaca
     3961 gaacaatgcc tcagaaattg accgcgtcaa ggatttttta atattgaaag atgcagataa
     4021 tacagccatg tacctggcac gcgagaaagc atcaggaaat gcctatacta tcagattgta
     4081 tataagtaaa gataaaggaa gtagttatac cctgataaag gattttggca acagaccact
     4141 gaatgaatta accatcacca acccgggcaa tacgaatgat gtgtatctga tagaaaagat
     4201 ttcacaactt gctactgcca tacacaagtg ggattttgta aacaatgatt ttgtaaaact
     4261 gaatcaggca aatttctcaa taggtggctc ttcatcattt aaggttacat cggcatggat
     4321 gactgatcat gtgcggtttt atctttataa ttcaacagaa ttcgtacatt atactgatga
     4381 ttatggtgtg acttgggtca acaaaggtaa actggctgct tcaccttggg gtgtaggtat
     4441 ctttgcattt ccttctgatc ctaaaaaatt gctaatgggt gaggtagaat gttacaggag
     4501 tgattttgaa ggcagtattt ggcaaaaaat caatggctgg ggcgaatatt atagcaaagt
     4561 aaagacaaag ctgcacgcag acatgatgca cttcaatgag tttcaaaaat cagacggaac
     4621 gaattttggg gtgatttgct gtcacggagg tatttataca actaaagatt atggcaaaac
     4681 cgttaacaac attacactcg aacatttgaa taatgctcaa tactatgatg ttgtaaccca
     4741 accgggtacg ttcgattata tatatgccgg cgcacaagat cagggctttc aaagagcaca
     4801 aagtaacatt gatgacaaat ctgctgccga ctttgtacag gtaatttcag gtgactacgg
     4861 gcacagttgt tttacaaaag gcggttccgg tctttggacg gtctatcctg gaggaagtat
     4921 ttcattctat aacaatccga aatcacaagg tggaccaact gcatattacg aacttgattc
     4981 ggataacgaa accgtgtgga ttccacccat tataccaggc ccgaataata atgcacatga
     5041 agtattttta gcaggtggca acaaagacgg tggtgcgggt tcgcatttaa tcaaattgac
     5101 ctataaacaa ggtaccatcg atgccacaca gtttgaattt gattttaaag caaattccgg
     5161 gtccgaaata tccgcaatgg ccatttctcc attcgattct actatatatt acgtagcaac
     5221 caacaatggc aaattctata tttcaccgga tgccggtgcc agcttctttg agagcagccc
     5281 gaaggtgcct ggtagccact atctgtatgg tgcatgtatt tatccatcca aactcaaccc
     5341 tgatgtcatc tatttgtccg gtagcggata ttccaattcc ggagttttgg tttctaccga
     5401 tggtggattc agtttcaatc cattgggtga aggattgcca aaaactttcg tattcaacgt
     5461 agtagcaaat gaagatgaat cgttgctgtt tgcagctaca gaagccggcc cttatgttta
     5521 ttcggttgct aagcaaaaat ggtttgatat gtcaggtatt gctgctccta ctcagacatt
     5581 ctggagtgtg gagtatatcc cgggacacaa agttgtcaga tttggtacat acggaagagg
     5641 tatctgggat tttagaattg atgatttgac tacttctgtt gcgcagtata attctgacaa
     5701 cagcttattg atgaccattg caccaaaccc agccagaaat tttcttaata ttaccttacc
     5761 tcaggatgaa actttcgatg ttgaaatatt caatatgaac gggcaagtgg tattaaaacc
     5821 cggtaagatt aatttagtaa aaaatattaa tgtgcaggca ttactgccag gtaattatat
     5881 catcaaagca agtagtaaaa aacgcaatat cagcaagata tttgtaaaac aataaagagc
     5941 tactctttaa aatgagaatg ccggaatcga actaaattcc ggcatttttt tatgtaactt
     6001 ttttgactca taaggttttt tccatcaaaa aatgtttgat tccaacttct gtaaagtctt
     6061 ttccttttat tgagtaatta agctttttgt aaaactttac ggctgtatcc cgggcatgaa
     6121 gaatgatttt gttgtagccc gatgattgtg cccagttctc tgtctctttt accatccgtg
     6181 tgcctatgcc tcttccttgt tgtgaatcat ctactgctac ctgacgcatt ttcaatacac
     6241 catcttcttt taattgatag ctgagacacg cgataagttt accttggagg ttataatacc
     6301 caaaatgaaa ttggtcaaat tcttccatca attgttcttc ggaaaactcg aggtcaagtg
     6361 gttttcttaa tatcttatat ctgaggctta ctgcttcatc atactccggt gtagcgaact
     6421 caatactggc aatcatttct ggattattta ggaaaaattt gcaaggctat ttccattgat
     6481 ttataaaaag cctccatatt taaattcaat tctacccttt tgtctgtaaa atattggatt
     6541 aggttttcgg ttggcatatt gcctgtaagg tcgtcttttg ccatcggaca accaccgaag
     6601 ccttttattg cagaatcaaa tctggtacag ccattggtat aggctgcatc aatcttttca
     6661 aaccaattgt gtggctctga gtgcaaatgg gcaccaaact ccacttccgg atagttaggt
     6721 ataagtttag aaaacaaata atcaatactt tcaggatttg acactccaat ggtatctgac
     6781 aatgacaaga tcgtgatacc catatcacga agtgccgaga cccatttctc tacaatctct
     6841 acgttccata catcaccata agggttacca aaacccatag aaatgtagat gactggtttt
     6901 ttgttgtatt ttaaacacaa ctcctgtatc tcctctaccc ttacgagcga ttcctctatg
     6961 gtagcattgg tattgcgtag ctgaaatgtt tcagaaatag aaaatggata accaagataa
     7021 tctatttgct caaaacggca ggcatcctcc gcaccacggg tattggcaat gatagccaat
     7081 agttttgaac gggttttgct caggtcgagc gatcctagta cttcggctgt gtcacgcatc
     7141 tgagggatag cttttggtga cacaaaacta ccaaagtcaa tggtatcata acccacctca
     7201 agtaaggctt gaatatatcg tattttatct tttgtctcga cgaaatcatg cagcccctgc
     7261 atagcatctc tcggacattc aattaattta atcattacaa cagccggatt gtattccaat
     7321 tacgtttcaa agataaggaa aattcaagga attttaaatt attgagatat ttagtaattt
     7381 gcaggctcat ttgattttag aaatgaaagg aatattatct ctcgttggtt ttcttatgtt
     7441 cagtacaggc attgttggtc tggtgttgag tcttgtaggt gtaagattat cggtcttatc
     7501 cttcatcgaa tccgctactc cgatgttggg ctttttgttt aagctgtttc tgtgcgtcgc
     7561 aggtataatt cttgtaatag tagcacgtac agactggaag aaagaaaacc aggcttagtc
     7621 agctataggc agtttgatac ctacggtaat accactatcg tggcgtttac catttagaaa
     7681 tacccaagta taatcgactc tgaataacct aaagatgctc cagcctatat tcgacaatcc
     7741 tgcagatatt tcggtatatc tgaagccatt ttcttgaaaa agcatattgg tagtaatatt
     7801 ttcttcgaat tttaattctt tgatgagtgg aattttactc agaaaaaaac cgccgaaatt
     7861 gtgccttgca tgtacttctg cgtatgtgcc caatgtactg taatcataat atggtaacaa
     7921 actaaaacct tcgcttcctg tcccggctat aaaagtctga ttgcctttaa aatgcttgta
     7981 atcaatgatt tcagccttat tacctgaata tatacttgta ccaccggtaa tactgatatt
     8041 tgtagtgccc aaaaaccgtg ggtaaaaatc atacttaacg gtaatctctg gttgctggat
     8101 ggtataatgc ccattggatc catcagtata tgaaagaact tttttaagat agtacctgaa
     8161 tgtgatgacc ggcaaggatt ggttaaaata ttgaacctga tcaggataag ttacatatcg
     8221 gatacccgga aaccatgtga tggaggcatt catacctata taatgatgtt ttggaaggat
     8281 accgaccgta tcgcccggaa aatttggaga atagacatct gatttctttc taaaactgta
     8341 atttgaggaa ttatcaaggc cagatcgtat tgcatattct gactccagat taagcttcaa
     8401 agtatgccac agcctttgtt cgtaagatac cttgccgaat gttttatcgt agagtttcat
     8461 ataattcagt ttgccataca aattatacaa gccattgact gtagccgata tcgggttggt
     8521 ttcattgtac tgagcagtaa tttttccaag cttgagtgcc agtttattgt aatgtaattt
     8581 attaaaaagt ctttcaaact caagagcacc tctccatttt ttatcagaga agccataact
     8641 cacttccggc ccaagtgaaa accaggaagt gttgttgtca tccttccatt tgcggtactt
     8701 cacattcaaa gctgaataaa atccctgtac cggattaaac tcaatagtag tgagtggtga
     8761 accaacattg agcgaccatc tacgaaacga attgtgatat gtatatccga aggtaaggtt
     8821 attagatgta aatttattat ttttacggtc tatactatcc cgaaaggctt tagaattcca
     8881 tattacctca aggctgtctt ttctgtgaaa atctctggat tcttcggcta agagaggaat
     8941 aggtcttagt gtgtcccaat atatatcgct ttttttgttc gagccggctt ctatttttac
     9001 caactcttta ctaaaaattg tattatcaaa gttgttattt aaggaataat tgccgaacac
     9061 ggctgtaaaa aagcctttta tttttattcc aagaagtcca agtccaaatt caatattctg
     9121 agaaaacaaa acccattgtc cttgctcatt tccaggtaca tactgctgac tgagccacat
     9181 tgtatcgagt atttcttgat tgatactttt tccggttacg ccaagatgac aagagtggat
     9241 gttccaggta ttttcataaa taaaaatatc gccccgaaac acaggtccac tttcattgcg
     9301 gggtattact ttaatcttgt taacctctcg gccatctgaa tcaaaaaaac ttccttcgag
     9361 tctgtacttg taaaacagca aagcttctgg cgcaatagga gaaatcagct gacgaccaaa
     9421 atcgacagcc acatcataaa aactaaagcg aatggcagta gcacgattga agctaaaacc
     9481 gcgactatcg ccactcacct ttgaagaaat aatctcttcg cgggtcttgt caggtgcctg
     9541 aaagtaaaat tttgagagtg actccgacag gtaaatgatt ccttgacggt ttgtatcaag
     9601 gattccgcca aggttaccca ggtcctcacc catcagtttt tgaggtattt ttactatttt
     9661 ctggttgcct tttatataga catcgcagga gtaagatttt atatgatttt tgaaatattc
     9721 gcgattagca atagcatttc tgataatggc ataagccgga tcttctccag aagcattaat
     9781 ttctacttca gctatctcca atgactgtgc ttcaagagta atattgagtt ttaacggtac
     9841 accggtataa ttaatttttt ttgatactgt cttgtaccca atatattgga agataacctc
     9901 cgattctccc tttgccaggt taagcgaata atatccattg acattggtat tgacacctga
     9961 ggtagaacca gctatataaa tactggctg
//
LOCUS       DAOQQY010000074         9807 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2566_length_9807_cov_5.243130, whole genome shotgun sequence.
ACCESSION   DAOQQY010000074 DAOQQY010000000
VERSION     DAOQQY010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2566_length_9807_cov_5.243130"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PLE23_05285"
     CDS             complement(<1..830)
                     /locus_tag="PLE23_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMX82713.1"
                     /translation="MYRNIIILMLVAGLNTNIATAQKGKALELDGVNSYMSVANNASL
                     NIASGQNFSVTCWIKSSSSADFFRIANKRGDQGNSSPGYEMIINKTSGQFGVNLRSTS
                     GTNAGPPFGNTPITDGVWHHVAFVVNADNSTVQIYVDGTLEKSQNSAAVGTESFANSE
                     QLYFGAAGGSKYYWNGLIDEIRMWSVPLSVADIQQDMTISVNGNETGLIAAWDFENEQ
                     GGVVPDLTGKHTGTLNGGAKCVSQATNDMIITEVVSYQPDIPIGRGEQNTRLASVNFK
                     T"
     gene            complement(990..1832)
                     /locus_tag="PLE23_05290"
     CDS             complement(990..1832)
                     /locus_tag="PLE23_05290"
                     /inference="COORDINATES: protein motif:HMM:NF022588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HMX82714.1"
                     /translation="MKRMSLLFCTFLFITGMNAQINTPQASPKGTVSQQIGLTDFSIT
                     YSRPSAKGRVVFGDVVPFGKTWRTGANQATLFTVSDDVTIEGQELKAGKYALYTVPEK
                     NTIEWIFYKNTTSWGLPEQWNDADVAIKVKTAFNTTANKTETLTFNFDELRNNSAVLK
                     LSWDNMEGVCKIAVNTDNKVLKDIEKALAGPSANDYYAAGKYYYESDKDLNKSYEWIH
                     KANEMSPKYFTLRQEALVLAKMKKYKEAVTVAQKSLELAKAAGSDEYVKMNTKDIADW
                     SKMK"
     gene            complement(1904..5938)
                     /locus_tag="PLE23_05295"
     CDS             complement(1904..5938)
                     /locus_tag="PLE23_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMX82715.1"
                     /translation="MKILYYTALIIILSTSAFSQQLPNLSLKTGEIFFKNFEQSECHE
                     LPHYRGYIYGIVRFDQIPNHFHLTRIKKSGLELSEFLGQTAYLCRFKQDDLENIRQLN
                     LVTGLATLHPERKITSTLREKFIKKTSEKKVHFSINIALSGVTEAQHFVTEMKAAGIV
                     LFQNGNAEHLLKGSLTSEQIYKVATHPYVVLIDNQAPPGEKEDLTARNLHRTSAIDQQ
                     FAQGLHYDGTGVNVLVRDDGKIGPHIDYQGRTNQYYAKGDGENSTHGDMVSGILMGAG
                     NLNPDVSGMARGAFLYITNYESEFTDVTMQLYAQNNVLVTSSSYSDGCNTGYTMNAQT
                     TDKQIFNNPDLIHVFSAGNSGQNDCNYGAGAGWGNITGGHKSGKNSIATGNVNQNGGI
                     DITSSRGPAYDGRIKPDICANGTDQLSTYPNNTINTGGGTSAAAPGISGSIAQLIHSY
                     RDMNNGATPPSALIKASMLNTATDLGNPGPDFTYGWGLVNTRKAYELLKAKQYKEIDL
                     SDSEQKEWKISVPNGTASLRVMVYWADKEASVLATKALVNDIDMQVISEDNTMYLPYV
                     LDNTPDPVKLSSPAKTGSDHLNNMEQVEIFNPSQGDYTVQLYGFQIPFGTVKAWLVYE
                     MSSTTPVLTYPNGGESFNPGENIYIHWDAIGNTGNYTIDYSADNGKSWNQVKTVGGNQ
                     KRTIWKVPDSPVDSALLRINRNGESDIVQAPFTILKQVSDLTIDNICPTTAQLSWSNI
                     VGASKYVLYELKEKYMEPFDTVTSNTAIINNYPGNVRWISVRPISQSGMPGKRSVATK
                     LHKLLNCKQQHDIAIYADTSTKINQVLCGPTQQVLGVMLKNEGSDTVKQINLHYIIDQ
                     NPVKTKHFSTMIAPDSSMVFHLDTIIPLQSYQFTMNVWADIPGETAHFNDSLLLNFNW
                     QVQDSEGADLPYSQDFESDDFPPIFWTTLSLNPEVNWDTLSCIGINGQQTRAAWMNNY
                     NNFATGTTDDLITQKFNLTNTQKAYLVFDYAYTGFDNTYNDTLEVDISNDCGKTYFQK
                     IIRTGGGDLQTTPNLETTFVPVSGYQWGTSVTDLGQYTGQSVSFRFRNISGYGNSLFI
                     DNIRVVNSYPIDPMVEFSSNKYCFGQQDEIKFFARPKPNAKYHWDFGTGALPTFSSGP
                     GPHYVKYTTPGKENVSLIVIPKFGNADTISVTDIDVYPETNAGFTFVKNGFEYSFSAD
                     QDITSSQHFWEFGDSQTSQETNPVHTYSTSGTYIVTHIITDSCGTDTSSHVLRTVSTN
                     DEFDQGLLVYPNPASAEVYIISNNTIQHIELYNLIGQLMPIKTANNQKLIKLDTSQLS
                     TGIYFVHIKLADTNLVRKIQIIRN"
     gene            6070..7308
                     /gene="pepT"
                     /locus_tag="PLE23_05300"
     CDS             6070..7308
                     /gene="pepT"
                     /locus_tag="PLE23_05300"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HMX82716.1"
                     /translation="MNFTVKDRFIKYVQIDTQSDPVSTSQPSTLKQLDLSKVLVQELK
                     EMGLSDVELDEFGYVYATIPATTSKKNVPVICFCAHVDTAPDASGTGVKPIIHSNYQG
                     QDIVLPDDTSVVIRMTDYPYLKDKIGHDIITASGLTLLGGDDKAGVAEIMDAAYQLIN
                     NPSIKHGKIRLLFTPDEEIGRGVDEVDIKKLGADFGYTLDGGPIGTLEDETFSADGAV
                     ITIQGVAAHPGYAKGKMENAVKIASDIVSALPKHRLTPETTQKKQGFIHPVKIEGGLE
                     NAKITFILRDFEEDLLKEKSEILRKVMDKVLLRYPHSSAKLEVFEQYRNMKQIVSGRP
                     EIIEYAKEAIKRSGLKPQLGSIRGGTDGSRLSFMGLPCPNLFAGEMAIHSRQEFVTVQ
                     DMQKAVETIINIVAVCEERS"
     gene            7730..>9807
                     /locus_tag="PLE23_05305"
     CDS             7730..>9807
                     /locus_tag="PLE23_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82717.1"
                     /translation="MKKHTPTIMPLIIRKCSLAFLVILLTGFASYAQEYKMFNGNIYT
                     CSGQITDSGGSSGNYGKNEKFTTTICSDNPQYSLLQIAFKTSDLKPGDELCIYDGINT
                     NFPLLSCSAQTGNFPYVIQSTKNNPAGCLTLVFTSDNFLQGSGFIGEISCKPVCQDVV
                     ASITSANNPNGVNAVIDLCAGEAIELKAVAAFPQNNQNYFQKAGACKYEWYLDNHLAA
                     TTETYNVPVFTEGGHSVNLLITDTLGCKSQNLALQKLRVAPKPTFFENNFEKLICVND
                     QLDISTAINLDKSKDLTLNENWAFFPQQDYVIDSVALPDGTGQAFSSVLFIGKFNPTE
                     IIDDPDDLDQVCITVEHSFLSDIEITLTCPSGQKIILQEQGKKTGVVHLGEPVEDDEL
                     LPDPIPGLGYLYCWQMGAPNGTWNDYVLKNSPKTLPAGVYAPYQSFENLYGCPIDGLW
                     TISVKDLWNNDNGYLFYWSMQFNPDLYPPVDSFTNNITSSKWLPNETLLSQSADSMST
                     EGIAPGAITHVWEVTDDNGCTFDYPVNLQVRPELDTKCGQCNIQLLDMADQDICASEQ
                     LVIQAETQPGGLQTKSDFSIMSSEVNTELSYKEFNIPVSLYNFPSTLNISTLINSVCL
                     TAEGTDLSKTDLSLVSPDGKEILLYAQNDINANAITQTCFLPSSLNPVTSGSGSYTGN
                     FKPANDFVQLT"
BASE COUNT         2804 a         1990 c         1967 g         3046 t
ORIGIN      
        1 gtcttaaaat tgacggaggc aagccttgta ttttgttcgc cacgacctat cggaatatca
       61 ggctggtaac tcaccacctc tgttatgatc atgtcattgg tagcttggct gacacatttg
      121 gctccaccat tgagcgtacc ggtgtgttta ccggttaagt caggaactac accaccttgt
      181 tcattttcga aatcccatgc tgcaataagc ccagtttcat taccatttac cgaaatggtc
      241 atgtcttgct gaatatcagc aacactcaaa ggcacagacc acattcttat ttcatcaata
      301 agaccattcc aataatattt gctaccaccg gctgcaccga agtaaagttg ttcgctgttt
      361 gcaaaacttt cagtacctac agctgctgag ttctgagatt tttcaagggt accgtcaaca
      421 tatatctgta cagtcgagtt gtctgcattg accacaaaag ctacgtgatg ccatactccg
      481 tctgtgatag gagtgttgcc aaatggagga cctgcattgg tacctgaagt gctccttaaa
      541 ttgacgccaa actgaccact cgttttgttg ataatcatct catatcccgg agaggaattg
      601 ccctggtcgc cacgtttgtt ggctattctg aaaaaatctg ccgaagaaga agattttatc
      661 caacaagtaa cactgaagtt ttgcccgctt gcaatattga ggcttgcatt attcgcaacc
      721 gacatatagc tattgacacc atccagttca agagcttttc ctttttgcgc tgtcgcaata
      781 tttgtattta accctgcaac cagcattaaa attataatat ttctgtacat agtctatggt
      841 attttatcta ttggttgata caattcactc tattacgaca aatataaaga atttgataag
      901 aaatttggaa aacatacata taaaaaacaa aaaagccgga atcattatta actccggctg
      961 gtaaaacttc aaacaaaatg gcttgagatt tatttcatct tagaccagtc tgcgatatcc
     1021 tttgtattca ttttaacgta ttcgtcacta cctgctgctt ttgccagttc aagtgatttt
     1081 tgggctacag ttactgcttc tttatatttc ttcattttag caagtacaag cgcttcttgt
     1141 ctgagtgtga agtatttagg agacatttcg ttagccttat ggatccactc gtatgacttg
     1201 ttgaggtcct tgtcgctttc gtaataatac ttgccagctg catagtaatc attagctgat
     1261 ggtccggcaa gtgctttttc aatgtcctta agtactttgt tatctgtgtt tacagctatt
     1321 ttgcaaacac cttccatgtt gtcccatgaa agtttcagta cggcactgtt gtttctgagt
     1381 tcgtcgaagt taaaggttag cgtttctgtt ttattagctg ttgtgttgaa ggcagtcttt
     1441 actttgattg caacgtctgc atcattccat tgctcaggca gaccccaaga tgttgtgttt
     1501 ttgtaaaaaa tccattctat tgtattcttt tcgggtacag tgtacagggc atactttccg
     1561 gctttcagtt cctggccttc gatggtaaca tcatctgata ctgtaaataa agttgcttgg
     1621 ttggcaccgg ttcgccatgt tttgccaaat ggtacaacat caccaaatac aacccgacct
     1681 tttgcacttg gtcttgagta agtaatgctg aaatctgtca aacctatctg ttggcttaca
     1741 gtacctttag ggcttgcctg cggtgtgttt atctgggcgt tcattcctgt gataaacaaa
     1801 aaggtacaga ataataaaga cattcttttc ataaataaaa gtttttagtt taataatcgt
     1861 ttgcttgctt taacaagatt tctgtatgaa tgttcttaaa tttttagttt cttataattt
     1921 gaatttttct gacaagattg gtatcagcta attttatatg tacgaaataa atacctgtac
     1981 tcagttgtga agtatccaat ttgatgagct tctgattgtt ggccgtcttt ataggcatca
     2041 attgtccgat caggttgtaa agctcaatat gctgaatagt attatttgag atgatataca
     2101 cttcagctga agcggggtta ggataaacta acaagccttg gtcaaattca tcatttgttg
     2161 acaccgttct cagaacatga gaggaagtat ctgtgccaca actgtctgta ataatatgcg
     2221 ttactatata agtacctgaa gtactatagg tatgaaccgg atttgtttcc tgcgaggtct
     2281 gcgaatcgcc aaattcccaa aaatgttgag atgaggtaat atcttgatca gcggagaaac
     2341 tgtattcaaa tccattcttt acaaatgtga aacctgcatt cgtttccgga taaacgtcta
     2401 tgtctgtaac cgagatggta tcagcattac caaacttagg tataacaatt aatgatacat
     2461 tttctttacc cggagtggtg tactttacgt aatgtggtcc ggggccacta gaaaatgtgg
     2521 gtaaagcacc tgtgccaaaa tcccaatggt acttcgcgtt tggtttcggc cttgcaaaaa
     2581 atttaatttc atcctgttga ccaaaacaat atttattaga gctgaattct accattggat
     2641 caataggata actatttaca acccggatgt tatcgataaa cagcgagttc ccatatccac
     2701 tgatgttcct gaatctgaag ctgacagact gtccggtgta ttggcccaaa tctgtaactg
     2761 aagtccccca ctggtatcct gagacaggca caaatgtcgt ttcaaggttt ggagttgttt
     2821 gtaaatcgcc gccacctgtt cttataattt tttgaaagta ggtttttccg caatcattag
     2881 aaatatctac ctccaaggta tcattgtagg tgttatcaaa acctgtgtag gcataatcaa
     2941 agactagata ggctttttgt gtatttgtca ggttaaattt ttgggtaata agatcatccg
     3001 ttgtgccggt agcaaaattg ttgtagttgt tcatccaagc cgctcttgtc tgctgcccgt
     3061 tgatgccaat acaactcaat gtgtcccagt ttacttccgg attaagactc aaagtagtcc
     3121 aaaatatagg tggaaaatca tccgattcaa aatcctgtga gtagggtagg tctgcccctt
     3181 ccgaatcttg aacctgccag ttgaaattca gaagaagact gtcgttaaaa tgtgctgttt
     3241 cacctggtat gtcagcccag acgttcattg taaattgata ggactgcaac gggataatgg
     3301 tgtcaagatg aaaaaccata ctgctgtcag gagctatcat tgtgctgaaa tgcttggttt
     3361 ttactgggtt ttggtcaatg atataatgta ggtttatttg tttgaccgta tcgctgcctt
     3421 cattttttaa cattacacca agcacctgtt gtgttgggcc gcacaatact tgattgattt
     3481 ttgtactcgt atcagcataa atggctatgt cgtgttgttg tttacagttc aagagcttat
     3541 gtagcttagt tgccacactt cgtttgccag gcataccgct ttgtgagatt ggccggacgc
     3601 ttatccatcg tacattcccg ggatagttgt tgatgatggc agtattgctt gtgactgtat
     3661 caaaaggttc catgtatttt tctttcagtt catacaatac atatttcgaa gcgcccacta
     3721 tattgctcca gcttagttgt gcggttgtcg gacagatatt gtcaattgta agatcgctaa
     3781 cttgttttaa gatagtgaat ggtgcctgta caatgtcaga ttcgccattt cggtttattc
     3841 tgagcaatgc actgtccact ggcgaatcag gtactttcca aatggtacgt ttttgattgc
     3901 cacctactgt ttttacctga ttccagctct tgccattatc tgctgaataa tcaatagtat
     3961 aattgccggt attacctatt gcatcccaat gaatgtagat gttttctccc gggttaaatg
     4021 attcgcctcc attcggatat gtaaggactg gcgtggtaga gctcatttca taaactaacc
     4081 atgccttgac agtaccaaag ggaatttgaa aaccataaag ttggacagta taatcgccct
     4141 gtgaaggatt gaaaatctct acctgttcca tattatttaa atgatcagag cctgtcttag
     4201 caggactcga tagtttcacc gggtcaggag tgttgtcaag gacgtaagga aggtacatgg
     4261 tattatcttc agatattacc tgcatatcta tatcattaac caaagctttt gttgccagta
     4321 ccgaagcctc cttgtctgcc cagtaaacca ttactctcag actagcagtg ccgttgggca
     4381 cggatatttt ccattctttt tgttcagaat cagacagatc aatttcctta tactgcttag
     4441 ctttcagcag ttcataagcc tttcgagtat ttaccagacc ccaaccataa gtaaaatcag
     4501 gacctggatt gcccagatcg gtggctgtgt taagcattga tgccttgatg agtgccgaag
     4561 gtggtgtagc accattgttc atgtcgcggt aactgtgaat gagctgggcg atggagccac
     4621 tgatgccggg agcggctgca cttgtacctc caccggtgtt aatcgtattg tttgggtacg
     4681 tagaaagctg gtcggtaccg tttgcacata tatcaggttt tattctgcca tcatacgcgg
     4741 gtccacgact actcgttatg tcaattccac cgttctgatt aacattaccg gtggcaatag
     4801 aatttttccc cgacttatgt cctcctgtaa tattgcccca tcctgcccct gcaccatagt
     4861 tacagtcatt ttgccccgaa ttgccggctg aaaatacatg tattagatcc ggattgttaa
     4921 atatctgctt gtctgtagtc tgagcgttca tggtgtagcc ggtgttgcag ccgtcactgt
     4981 aggagcttga tgtgaccaac acgttgtttt gagcatagag ttgcatggtg acatccgtaa
     5041 attccgattc gtaattagta atatacaaaa aagcgcctct tgccatacca gaaacgtcag
     5101 gattcagatt gccggctccc attaagatgc ccgaaaccat atcgccatgg gtagaatttt
     5161 caccgtcacc tttggcgtaa tattgatttg tccgaccttg atagtctata tgagggccta
     5221 ttttgccatc atctcttacc agaacattta cacccgtgcc atcataatgg agcccttgtg
     5281 caaactgctg atctatggca cttgtacggt gaaggttcct tgctgtaagg tcttctttct
     5341 ctcccggagg agcttgattg tcaatcaata ctacatacgg atgagttgcc accttgtata
     5401 tttgttcgct tgtaaggctg ccttttaaca aatgttcggc attgccgttt tggaacagaa
     5461 caatgcctgc tgccttcatt tctgtaacga aatgctgagc ttctgtgaca ccagaaagtg
     5521 cgatgttgat agaaaaatgg acttttttct cggatgtttt tttgataaac ttttccctca
     5581 aggtcgaggt aatttttcgt tccgggtgca atgttgctaa ccctgtcact aaattgagtt
     5641 gtctgatatt ttctaaatca tcttgtttga agcggcacaa atatgcagtt tgtcctagaa
     5701 attcagacaa ttcaaggcca gatttcttga ttcttgtaag atgaaagtgg ttcggtattt
     5761 ggtcaaaccg tacaatacca taaatatagc cacggtaatg tggtagctcg tgacattcag
     5821 attgttcaaa atttttgaaa aatatttcac ctgtttttag tgataaattt ggcaattgct
     5881 gcgaaaatgc cgaagttgac aaaataataa tcaaagcagt atagtacaga attttcataa
     5941 tataaatatt ttcttatttc taattgcctg atttactata caaaaatacc gaaaagtttc
     6001 catggttttg ctttttattg aaatttattg attacatttg tcgctgatta gttgctccaa
     6061 gaaatgaaaa tgaattttac agtcaaagac agattcataa aatatgtgca gatagatacc
     6121 cagtcagatc cggtaagcac atcgcaaccc tctactttaa aacaacttga cttatcaaaa
     6181 gttcttgtac aagagctgaa agaaatgggc ctcagtgatg tagagctcga cgaatttggt
     6241 tatgtgtatg caactattcc ggccactaca tcaaagaaaa atgtacctgt tatatgtttc
     6301 tgtgctcatg ttgacaccgc tccggatgcc agcggtactg gcgtcaagcc tatcatccac
     6361 agtaattacc aaggacagga tattgtgtta cccgatgata cttcggtagt aatacgtatg
     6421 accgattatc cttacctgaa ggataaaata ggtcatgaca tcattaccgc aagcggactt
     6481 actttgttgg gaggtgatga caaggccggt gtagcagaaa taatggatgc agcataccag
     6541 ctaatcaaca atccttctat caaacacggt aaaatcagac ttctgtttac gcctgatgaa
     6601 gaaatcggcc gtggtgtgga tgaagttgat atcaaaaaac ttggtgccga ttttggctac
     6661 acgcttgatg gtggcccaat cggtacattg gaagacgaaa cattttcagc agatggtgct
     6721 gtcataacca tacaaggcgt agcagcacac ccgggctatg ctaagggcaa aatggaaaat
     6781 gctgttaaaa tagcatctga tattgtatct gcactaccaa aacaccgact taccccagag
     6841 acaactcaga aaaaacaagg gttcatccat ccggtaaaaa tagaaggtgg acttgaaaat
     6901 gcaaaaatta cctttattct gcgtgatttt gaagaagatt tgttgaaaga aaaatcagaa
     6961 attcttcgca aagtgatgga taaggtgctg ctgcgttatc cacattcatc tgcaaaactt
     7021 gaagtattcg agcagtatcg aaatatgaag caaatagtat ccggcagacc ggaaattata
     7081 gaatatgcca aagaagccat caaaagaagt gggctgaaac cacagcttgg cagtatcaga
     7141 ggtggaactg acggatcaag gctttcattt atgggacttc cttgccctaa tctctttgca
     7201 ggcgaaatgg caatccattc aagacaggaa tttgtaacag ttcaggatat gcagaaggca
     7261 gtagaaacta tcattaacat cgttgccgta tgtgaagaaa ggtcctgagc tattcagatt
     7321 taccaacaaa ttgaacccaa aaaccatata acaaataaca aaaaccgaac aaagagtatc
     7381 taagtttctg tatttaaaca tgatgatatt cgttgattta aggaaggaag gatgattcct
     7441 tttcattatt caaatgtgcc gttgaatgcc ctttaacagc ctgtgaataa gtggtaaatg
     7501 ttaaaatttt taaagagcct taactttggt aaggtatttg gtataaaaat tcaggtcatc
     7561 cttgaacata tcaaaactac cagattagga tttttaacta ataaatgctt aatttattat
     7621 gcttgtagta ttgattttga ggggaataaa taacaacatg aatatgcttg atatcaaggg
     7681 tatgaatttt cggtctttaa ctaactacac aaacgcctaa agccggaata tgaaaaaaca
     7741 tacacctacc atcatgccac taatcattcg gaagtgtagt cttgcatttc ttgtgatatt
     7801 actcactggt tttgcttctt atgctcagga gtacaagatg ttcaacggga atatctatac
     7861 ttgttcaggc cagattacgg actcaggcgg ctcatccggc aactacggta aaaatgaaaa
     7921 attcaccact actatttgtt cagataatcc acaatattct ttattacaga tcgctttcaa
     7981 aacttctgac ttgaagcccg gagacgaact ctgtatttac gatggtatca atacaaattt
     8041 tcctttactc tcgtgttctg cacaaacagg aaatttccct tacgtcattc aatcaacaaa
     8101 aaataacccg gccggttgtc tcacattggt cttcacgtcc gacaattttc ttcagggttc
     8161 aggattcatt ggcgaaatat cttgtaagcc tgtttgccag gacgtggtgg catccattac
     8221 ttctgccaat aaccctaatg gggtcaatgc agtaattgat ttatgtgccg gtgaggcaat
     8281 agaactcaaa gcagtagctg cttttccaca aaataatcag aattatttcc aaaaagccgg
     8341 tgcttgtaag tatgaatggt atcttgataa tcatcttgct gctacaactg aaacttataa
     8401 tgtacctgta tttacagaag gtgggcattc ggtcaacctc cttataaccg atacacttgg
     8461 ctgtaaaagc cagaatcttg cattacaaaa gctcagagtt gctcctaaac caacgttttt
     8521 tgaaaataat tttgaaaaac tcatctgtgt taacgaccaa ctggatataa gtacagccat
     8581 caatcttgac aaatcaaaag atctgacact taacgaaaat tgggcgtttt ttccacagca
     8641 agattatgtg atagattctg ttgcgttgcc tgatggtaca gggcaggcct tctcatctgt
     8701 tttgtttata ggcaaattta acccaacaga aataattgat gacccggacg atctcgatca
     8761 ggtatgcatc actgtagagc attctttcct tagtgatatc gaaatcacac tcacatgtcc
     8821 aagcggacaa aaaataattc ttcaggaaca aggcaaaaaa actggagtgg tacatctcgg
     8881 tgagccggta gaagatgatg aactcctccc ggatcctata ccggggcttg gatatttgta
     8941 ctgttggcag atgggcgcac cgaatggtac atggaatgat tatgttctta aaaacagccc
     9001 caaaacactc ccggcaggtg tttatgcacc gtaccaatca ttcgaaaacc tctatggatg
     9061 cccgattgac ggtctttgga caatatctgt taaagacctc tggaataatg ataacggcta
     9121 cctattttat tggtctatgc agttcaatcc ggatctttac cctccggttg attcatttac
     9181 caacaatatt acatcaagca aatggctgcc gaacgaaacc ctgctgtcac agtcagccga
     9241 ctcaatgtca acagaaggta ttgcgccagg agccatcaca cacgtatggg aggtgacaga
     9301 tgataacggt tgtacgttcg attatcctgt taatcttcag gtaaggcccg aacttgatac
     9361 caagtgtggc caatgcaata tccagcttct cgatatggca gatcaggata tctgtgcaag
     9421 cgaacaactc gtcatccagg ctgaaacaca accgggtggc ctgcagacaa agtcagattt
     9481 ttcgattatg tcatcagaag tgaacacaga acttagttac aaggaattta acattccggt
     9541 gagtctgtat aactttcctt ctacgctcaa catttcaaca ctcataaatt cggtgtgcct
     9601 aacggctgaa ggaacagatt tgtccaaaac tgatctcagt cttgtgagtc cggatggcaa
     9661 ggagatttta ctttatgcac aaaatgatat taatgccaac gctattacac aaacctgttt
     9721 tctgccttca tcccttaatc ctgtcacaag cggtagtggt agctataccg gaaatttcaa
     9781 accggcaaac gattttgtac aacttac
//
LOCUS       DAOQQY010000075         9777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2576_length_9777_cov_6.090208, whole genome shotgun sequence.
ACCESSION   DAOQQY010000075 DAOQQY010000000
VERSION     DAOQQY010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9777
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2576_length_9777_cov_6.090208"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="PLE23_05310"
     CDS             <1..735
                     /locus_tag="PLE23_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMX82718.1"
                     /translation="APKFKKMSLELGGKNPNIIFADCNYELMLKTTVNSSFSNQGQIC
                     LCGSRIFVEASIYQKFKKDFVEAVEKLKLGDPNDSDSKIGAVVSKPHMEKILSYIELA
                     KQEGGVILTGGGQVSLPGRCAEGYFISPTVIEGLDYQCRTNQEEIFGPVVTITPFYTE
                     EEALTMANSTEYGLASSIWTENITRATRMAEKIEAGIVWINCWLLRDLRTPFGGVKNS
                     GLGREGGTEALEFFTEPKNVCIQYGH"
     gene            complement(751..2241)
                     /locus_tag="PLE23_05315"
     CDS             complement(751..2241)
                     /locus_tag="PLE23_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82719.1"
                     /translation="MTIKILRFLIGIFFISLFFIEMTSCRKQQDFFKDSSAMVVFSRD
                     TLSFDTVFTTQGSATRLIKVFNSYDQPILLSKVWLEKQNTSKFNLNVDGIPGRTVTDV
                     EIEAKDSIYIFVEVTINPDDPVSISPFVVEENLFVEVNGNTQKVILEAWGQNANYIPN
                     NTNSNGLAITTCNLGSLSWNDPKPYVIFGVLFIDSCTVNIPAGTKIYVHGGIANNSLG
                     KYSDGIIYVLPNGKLNIQGTKENPVIIQGDRLEKEFSDIQGQWAGIRFAPGSKNNIIR
                     YAEIKNSIVGVRADSASQVRMYNTKIYNTSSSGVIAEHCIFVAENCLFYNNGGNAVQT
                     EFGGDIFLRYCTMASYGNNQAALRLSNTRCLDQFCNTFATYPLNADVQNCIIAGSQED
                     ELSFLNKSQNQADFVLKMKNNIVRAKDILKQDAYPDFFDKICNSCISLKPSDKLFRNI
                     SKQMYQLDTLSVAEQRAVPIPLITKDIEDADRDPVTPDIGAYEYQY"
     gene            2866..3642
                     /locus_tag="PLE23_05320"
     CDS             2866..3642
                     /locus_tag="PLE23_05320"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HMX82720.1"
                     /translation="MSISRACRIVGLNRSMFYYRTIKNDGSVIDRLNQLAERYPTRGF
                     DTYYGKIRLEGIKWNRKRVLRVYRMLNLKMRRKRKQRLPSRIKQTLGVPARINQTWSI
                     DFMSDSLTNGRRFRILNVIDDCNREALISEAYYSFPAIRLVNRLKELIIERGKPKRIR
                     TDNGPEFISKVFTSFCEENNIKLQFIQPGKLAQNAYIERLNRTFREDVLDAYLFETIY
                     QVNALAFEWQMDYNQNHPHKALNGMSPWIYINKILNMNIN"
     gene            3818..5605
                     /locus_tag="PLE23_05325"
     CDS             3818..5605
                     /locus_tag="PLE23_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82721.1"
                     /translation="MAKKISVIALCFCYIIMIYTAFIFYPKWKQSGTEATISWDASGY
                     YMYLPALFIYKDIKKCAFKDSILAKYSPTPDFQQAFVHTKSSNYVMKYSSGQAITMMP
                     FFFMAHLWCKLNPAYPADGFSYPYQFAIGVGMLFYAFIGLFFLRKILLHFFKDSTVAI
                     LLLCYAIGTNYLNYSSIDQAMTHNVLFTIYCLNIFLTIIFQKSPSYKSAFSIGILIGL
                     ATLIRPTDIISALIPLLWTINSSKDLIPKIRFIRQHFSKHLIIAVSALAVFSIQLIYW
                     KWVANEWFVYSYQDQGFSWLHPHLYNYTLSYKCGWLRYCPMMILPLTGLYFYLKTGVN
                     KVAIIAFTIINLYIVTAWDIWDYGNTAGRAMIQSYPILAFPFCALIERMTKNKRTATI
                     LAIPVLFFTYMNIWWTYHAHAGNTQITDVTREYYWKVAGRWNESDEDKKLLDNPHAFY
                     GTPKNEIILYRNSFDNDTSQNAVQETNNNLIKINKELQQTSLYSIQNNSSFKKWIRAS
                     ADFRCTIKEWDIWKQAQFIVQFYDKDTVIQTNLIHVHRFISDGETENIYLDAIVPVVK
                     WDKLTISIWNANGDKELFIDNLMIQSFND"
     gene            5840..7480
                     /locus_tag="PLE23_05330"
     CDS             5840..7480
                     /locus_tag="PLE23_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMX82722.1"
                     /translation="MKKIFNKPILIAGFIALTGIAAINSPFVTGNYFEITKNIELFAN
                     TFKELNTQYVDELDPGKLMKTGIDAMMKSLDPYTNYYSESQVEGFRYITEGKYNGIGV
                     RFRLRDDYIMIMAPMDGSPAQKAGLKAGDMIMEVDGKSAKGKTEDELNSILQGFPGSE
                     VTLKISRPATGEMTLSVKRDEVNTKNVPHFEEISPNIGYIALTTFTKNAANNILDALK
                     DLKSDNPNLKGLILDLRDNGGGLLNEAVSICNIFVPKSEEIVSTRGKVKDRDMVYRTT
                     MNPSDEKIEIVVLVNKMSASASEIVSGALQDLDRAVIMGQRTFGKGLVQNFREIGYNA
                     RIKVTTAKYYVPSGRCIQAVSYVNGEPVPVADSLQATFKTRNGRKVKDGGGVQPDVEI
                     PITDKTDLMKALDDKLMIFDFATDYVNKNPTPVDVKTFSFIDWDAFLKFLKDKKFTYE
                     SKTEHLLSQLKTEAEKSKMFSTVQNEYSNLKNKIESEKQKDLAANKDRILSLLSGEIA
                     SRFYYEKGRVQVKLKNDNEIKDAVELLNNPAKYNKILK"
     gene            7573..8298
                     /locus_tag="PLE23_05335"
     CDS             7573..8298
                     /locus_tag="PLE23_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HMX82723.1"
                     /translation="MKKYITGFTFILCALFMASCTPAELQKAMDAANSMQGGGKNETA
                     EGLKQALQIGVSNGSSQLSQTNGYLNSIYKIVLPPDVAKIADKLKVIPGFNNVEAQLI
                     EKFNAAASDAAKSAKPIFVDAIKAMTIQDALGILMGNQDAATRYLENNTRTKLYGQFN
                     PVIVQSLNKFGALDYYKQIVDKYNSLPLVNKVNPRIDDYITNKALDGLFGMIQGEELQ
                     IRKDPAKRVTELLKRVFSKQDKK"
     gene            complement(8354..9469)
                     /locus_tag="PLE23_05340"
     CDS             complement(8354..9469)
                     /locus_tag="PLE23_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMX82724.1"
                     /translation="MRINARTLLIIGATLLGLYLVYKLSDIVSYVLIAWVLSLLGRPV
                     SEFLKKILTFRKIKPGNTLLAFLTMLLFFLVLTILFWLFVPLVIEQANNLSKVDISAL
                     NNTLHIPLNELKNWLVQYGLISPTVDIDQQILANIKKFIEPSKVGAFFTSAVGAVGNA
                     VIAATSIMFILFFFLKDQTMFDDFLKSLLPKKYEDHVIKALHSISTMLRSYFGGIVLQ
                     VSILTVLISVALTILGIKNALLIAFFGALMNVIPYIGIVIAMLFGVMITISSNLDLNF
                     YTEIVPMIIKVLLTFGATQLVDNFLVQPYVYSKSVDAHPLEIFLVIMVAGKTGGILAM
                     LIAIPSYTALKLVAKVFFNQWRIVQHITGGLDEPDRE"
     gene            complement(9501..>9777)
                     /locus_tag="PLE23_05345"
     CDS             complement(9501..>9777)
                     /locus_tag="PLE23_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HMX82725.1"
                     /translation="KSRNLTAYTEYLIKAIDSDAIELITPTEPGQRGCQLSIRVKGAD
                     KTLFGKISEAGVIADWREPDVVRIAPVPLYNSYLDVFRFVQILRASL"
BASE COUNT         3160 a         1985 c         1922 g         2710 t
ORIGIN      
        1 gcaccgaagt tcaaaaaaat gtcgctagag ttaggcggaa aaaatccgaa catcatattc
       61 gccgactgca attacgagct gatgctcaaa acaacggtca attcttcttt ttcgaaccaa
      121 ggacagatat gcctttgcgg tagccggata ttcgttgagg catccattta ccagaaattt
      181 aaaaaagatt ttgtggaggc agttgaaaaa ctgaaattgg gcgatccgaa tgatagtgac
      241 agtaaaattg gggcagtcgt gtcaaaacca cacatggaaa aaatcctttc atacatagag
      301 ctggcaaaac aagaaggcgg tgtcatacta accggcggtg gccaggtaag cctaccgggg
      361 cgttgtgctg aaggttactt cataagccca acggtcattg aaggtcttga ttaccaatgc
      421 cgtaccaatc aggaagagat tttcggtcct gtagtgacca ttacaccatt ttacacagaa
      481 gaagaagcat tgacaatggc taacagtacc gaatacggcc tagcgagcag catctggaca
      541 gaaaacataa caagagcaac ccggatggca gaaaaaatag aagccggcat cgtctggatc
      601 aactgctggc tattacgcga tttgcgaaca ccatttggtg gcgtaaaaaa ctcaggctta
      661 ggtcgcgaag gtggtacaga agcactcgaa ttctttaccg aacccaagaa cgtctgtatt
      721 cagtacggtc attgaacctg agcatccatc tcagtactga tactcgtagg cacctatatc
      781 cggtgttaca gggtcgcggt ctgcgtcttc aatatctttg gtgatcaatg gtattggcac
      841 tgccctttgt tcagcaaccg ataaggtatc aagttgatac atctgtttgc ttatattcct
      901 gaacaacttg tcagaaggct tcaggctaat gcacgagttg catattttat caaaaaaatc
      961 agggtatgca tcttgcttca gtatatcttt cgcccttacg atattgtttt tcatttttaa
     1021 tacaaagtca gcctgattct ggcttttatt caggaatgaa agttcatctt cctgcgaccc
     1081 tgcaatgata cagttctgaa catcggcatt caacgggtag gtagcaaaag tattacaaaa
     1141 ctgatcaaga cagcgcgtat tagacaatct gagtgcagct tgattattgc cgtagctcgc
     1201 catggtgcaa tatctgagga aaatatctcc accgaattca gtttgtaccg cattgccacc
     1261 gttgttgtag aacagacaat tttcggcaac gaatatacaa tgttcggcaa tgacaccact
     1321 ggacgaagta ttataaatct ttgtattgta cattcttacc tgagaggcag agtcggcacg
     1381 tacgcctaca atcgaattct tgatttcggc atagcggatg atgttgttct tgctgccggg
     1441 cgcaaacctg atgcctgccc actgaccctg tatgtcagaa aactcttttt cgaggcggtc
     1501 tccttgtatg atgacggggt tttccttcgt accctgaatg ttgagtttgc cattcggcaa
     1561 tacatatatg ataccgtcac tgtatttacc aaggctgtta ttggcaatgc cgccatgaac
     1621 gtatattttc gttccggcag gaatgttgac cgtacagcta tcgatgaaca gtacaccaaa
     1681 tatcacataa ggcttcggat cgttccagct caggctaccc agattgcagg tggtgatggc
     1741 aagtccgttg ctgttggtgt tgttcgggat gtagttggca ttctggcccc aggcttcgag
     1801 aataactttt tgagtattgc cgtttacttc aacgaaaaga ttttcttcaa ccacaaacgg
     1861 gctaatggaa accggatcat cggggttgat ggttacttct acgaagatgt agatactgtc
     1921 cttggcttcg atttcaacat cagtcaccgt tctacccgga attccgtcaa catttaggtt
     1981 gaatttggag gtattctgtt tttcaagcca caccttgctc aacaatatgg gctggtcgta
     2041 gctgttaaat accttaatca gccgagtagc agaaccctgt gtcgtaaaaa cggtatcaaa
     2101 actcagcgta tctctggaga acaccaccat ggcactactg tccttaaaga aatcctgttg
     2161 ctttcggcag gaggtcattt caataaaaaa taaagaaatg aagaaaatgc ctatcagaaa
     2221 tcgtaatatc ttaattgtca tatttcggtt tgattggtaa agttacgtca gagttgaaca
     2281 ttttgttgtt cctggctcaa gttttatatc taaagagaat tttgaaagat ttacaatttc
     2341 aacattacta attttatatt tgcctgacgt tattatcaag taattctata agaatacttt
     2401 aatgattaaa aaatacacat ctatcgctct gttttcctct attccatatc taggtaaact
     2461 tccccatttt tgtagccttc ccttttgggt aaacttcccc atttttcaga cagtttaaaa
     2521 atataataaa tgattaaatt tactgtcatg aagagatcaa aatttactga gtcggaaatt
     2581 atcaatattc ttaaagaata tgaatttgga aagagtgtta aagacatatg ccgtgaatat
     2641 ggtatagcaa ctcctaactt ttactcatgg aagcataagt atagcgggat ggacactcaa
     2701 cagctgaagg agctcaaagc cttacaagag gaaaatgctc gcttaatagc gtatgtatgc
     2761 tgacttgagt ttagaccatc gtatcttaaa ggatattttt gaaaaaaagc tttagcgccc
     2821 tgccatcgta aagaactggc cgaacaggcg gttgaaaaag aatcaatcag cattagcagg
     2881 gcgtgcagaa tagttggtct taaccgaagt atgttctatt atcgaaccat taaaaatgac
     2941 ggttctgtaa tagaccggct taatcagttg gctgaaagat atccaaccag aggttttgat
     3001 acttattatg ggaaaatacg cttggaaggt attaaatgga ataggaaaag agttttgaga
     3061 gtttatagaa tgcttaattt aaaaatgaga agaaagcgaa aacaaaggct tccctcccga
     3121 ataaagcaaa ctctgggggt gcctgctcgg atcaatcaga cttggtctat tgactttatg
     3181 agtgatagcc tgaccaatgg aagaagattc agaatattga atgtaattga tgattgcaac
     3241 agagaggccc tcatcagtga ggcctattat tcgtttccgg cgataaggtt agtcaatcgt
     3301 ttaaaagaat tgattattga gagaggaaaa ccaaaaagga ttagaacaga taatggtcct
     3361 gagtttatat caaaagtatt cacatcgttt tgtgaagaaa acaatattaa gctacaattc
     3421 attcagcccg gaaaactggc gcaaaatgct tatatcgaaa gattgaacag gacctttaga
     3481 gaagatgtat tagatgctta tttgtttgaa acaatctacc aggtcaatgc gttggctttt
     3541 gagtggcaga tggattacaa ccagaatcat ccgcacaaag ctttaaatgg aatgagtcct
     3601 tggatttata taaataaaat tttaaatatg aatattaact gatttttata cctttaccgt
     3661 gtctgacaaa agggaaggct acagacaaaa aattaaagaa atcaatagtt tattggcact
     3721 atgtactatt cctatacaag acttaccaaa tggtatgtat gtgattaaaa ttgagaacag
     3781 taagaagaca actacctata aattttataa aaactaaatg gcaaaaaaaa tatccgtcat
     3841 tgctttatgt ttttgctata tcattatgat atacacagcg ttcatatttt acccaaaatg
     3901 gaaacagtca ggaacggagg caactatcag ctgggacgct tcgggctact atatgtatct
     3961 tcctgcatta tttatctata aagatataaa aaaatgtgcg tttaaagata gtattttagc
     4021 aaaatactcc ccgacccctg atttccaaca ggcatttgtc catacaaaat caagcaacta
     4081 tgtaatgaaa tattcgagtg ggcaggccat aactatgatg ccgttttttt ttatggctca
     4141 tctctggtgc aagctaaacc ctgcataccc ggccgatggg ttttcttacc cctatcagtt
     4201 tgctataggt gtcgggatgt tattttatgc ttttatcggg ttattctttt taagaaaaat
     4261 tttattacat ttttttaaag acagtactgt tgccattctg ctattgtgct atgcaatagg
     4321 cactaattat ttaaactatt ccagtataga ccaggcaatg acccacaatg tattatttac
     4381 aatctattgt cttaatatat tcttaacaat aatttttcaa aaaagcccct catataaatc
     4441 tgccttttct atcggcatac tcatcggatt agcaacactc atcaggccaa cagatattat
     4501 ttctgcgctg attcccttac tctggactat taactcttct aaagacctga tacctaaaat
     4561 cagatttata cggcaacatt tttcaaagca tcttataata gccgtttctg cattggcagt
     4621 tttttcaata caactgattt attggaaatg ggtagcgaac gaatggtttg tttatagcta
     4681 tcaggaccaa ggttttagct ggcttcaccc tcatctttat aattatacat taagctataa
     4741 atgtggttgg ctacgatatt gtcccatgat gatattgcca ctaacaggtc tctattttta
     4801 tttaaaaacc ggagtgaaca aagtagcaat aatagctttt actataataa atttatacat
     4861 agttactgct tgggacattt gggattatgg aaatacagcc ggtagagcta tgattcaatc
     4921 ttaccccatt ttagcatttc ctttttgtgc tcttattgaa cgtatgacaa aaaataaacg
     4981 gacagccaca atattagcta ttcctgttct tttttttaca tatatgaata tatggtggac
     5041 atatcatgcg catgcaggga atacacaaat cacagatgta acaagagaat attactggaa
     5101 agtagcagga aggtggaacg aaagtgatga ggacaaaaaa ctacttgaca acccacatgc
     5161 tttttatggc acacctaaaa atgaaataat attataccgt aattcttttg ataatgacac
     5221 ttctcaaaat gcagtacagg aaacaaacaa taatttaatc aaaataaata aagagttgca
     5281 acaaacttca ttatatagca tacaaaataa ttcaagcttt aaaaaatgga ttagagcttc
     5341 agctgatttt cgttgtacca taaaagagtg ggatatatgg aagcaagcac agttcattgt
     5401 acaattttac gacaaggata cagttataca aaccaattta atacatgtac atcggtttat
     5461 atctgatggc gaaactgaaa atatttattt agatgcaatc gttccggtag taaaatggga
     5521 taaactaact atttcaatct ggaatgccaa tggcgataaa gagttattta ttgataatct
     5581 tatgatacag agctttaatg actaatagta tagtttcaac acattgttgt tgattttcca
     5641 aatgagtcag tatcacatgc aacccgctct aaagcaactt agctttactg atttattctc
     5701 ttgtttattt tacgtttagg acggattatt actcacacaa aactgatgtc tcacaattca
     5761 ggagcccaat ccaacaatta aacaagtgtt gcgttattta taagcaaata cattttagtc
     5821 agacatcttt cattgattta tgaaaaaaat attcaacaaa ccgatactca tagccggatt
     5881 tatagcactt acaggcatcg cagccatcaa ttcaccattt gttaccggca attacttcga
     5941 aataacaaaa aatatcgaac tgttcgccaa caccttcaag gaactgaaca cgcagtatgt
     6001 tgacgagctg gatcccggaa agctcatgaa gacgggcatc gacgccatga tgaaatcgct
     6061 ggatccatac accaactatt attccgaatc gcaggtggaa ggcttccgat acatcacaga
     6121 aggaaaatac aacggaatcg gtgtcaggtt tcgcctcaga gacgattata tcatgattat
     6181 ggcgccaatg gatggcagtc cggcacagaa agcaggtctc aaggcgggtg atatgataat
     6241 ggaagtggat ggcaaatcgg ccaaaggaaa aacagaagac gaactcaact ccatccttca
     6301 gggatttccg ggatctgaag taacgctaaa gatatcgcgc ccggctaccg gtgaaatgac
     6361 tttatctgtc aagcgtgatg aagtgaacac taaaaatgtt ccacatttcg aagaaatttc
     6421 gcctaatatc ggctatatcg cacttactac atttaccaaa aatgcagcca acaacattct
     6481 ggatgctctg aaagacctga aatcagacaa tccgaatctg aaaggcctga ttctcgactt
     6541 gcgcgataat ggtggtggct tgttgaacga agcggttagc atctgcaaca tattcgtgcc
     6601 caaaagcgaa gaaatcgtat ctacccgtgg caaggtaaaa gaccgcgata tggtgtaccg
     6661 tactactatg aacccaagtg atgaaaaaat agaaatcgtg gtgctcgtaa acaaaatgtc
     6721 ggcatccgct tctgaaattg tctccggtgc attgcaagac ctcgaccggg ctgtcatcat
     6781 gggtcagcgg acattcggta agggtctggt acagaatttc cgcgagattg gctacaacgc
     6841 ccgtataaaa gtaacaacgg ctaaatacta cgtgccaagt ggccgttgca tacaggcagt
     6901 ctcttacgtc aatggcgaac ctgtgccggt ggctgactca ctgcaggcga ccttcaaaac
     6961 cagaaacggc cgcaaggtca aggatggcgg tggcgtacaa cccgatgtcg aaatcccgat
     7021 tacagacaaa acagacctga tgaaagcact cgatgacaaa ctcatgatat tcgatttcgc
     7081 aacagattac gttaacaaaa atccaactcc ggttgatgtg aagactttta gctttatcga
     7141 ctgggatgca ttcctgaaat tccttaaaga taaaaaattt acatacgaat caaaaacaga
     7201 acatctcctg agccagttga agacagaagc cgagaaatca aaaatgttct ctaccgtaca
     7261 aaatgaatac tccaacctga aaaacaaaat agaatctgaa aaacaaaaag accttgccgc
     7321 caacaaagac cgtatcctga gcctccttag tggcgaaatt gcctctcgct tttattacga
     7381 aaaaggcaga gtacaggtaa agttaaaaaa tgataacgaa atcaaagatg cagtcgaact
     7441 gttgaataat ccggcaaaat acaacaagat tttaaaataa gcccggacag ctcagcttgt
     7501 taaaaaaata ctaattccaa cgtaaaggaa ttacttttgt aaaataatac gtcttaataa
     7561 taaaaaacaa aaatgaaaaa atacatcaca ggttttacat tcatcttatg cgcactcttc
     7621 atggcgagtt gtacacctgc cgaactacaa aaagctatgg atgcagccaa ttcaatgcaa
     7681 ggtggaggta aaaacgaaac cgcagaaggt ctcaaacaag cattacagat aggtgtgagc
     7741 aacggttcga gccagctgtc acagaccaac ggctatctga acagtattta taaaattgtg
     7801 cttccaccag atgtagccaa aatcgcagac aaactaaaag taatcccggg attcaacaat
     7861 gttgaagccc agcttattga aaaatttaac gctgctgcct ctgatgccgc caagagtgct
     7921 aaaccaatat tcgtagatgc catcaaagct atgaccatcc aggacgcact cggtattctc
     7981 atgggcaatc aggatgctgc cacacgttac ttagaaaaca acacccgtac taaactctac
     8041 ggacagttta atccggttat cgtacagtct ctgaacaaat ttggcgcact cgattactac
     8101 aaacaaatag tggacaaata caacagtctg ccactggtca acaaggtaaa tccacgcatc
     8161 gacgactaca ttaccaacaa agcactcgac ggtctgttcg gcatgatcca aggcgaagag
     8221 ttgcagataa gaaaagaccc tgccaaacgt gtcaccgaac tactgaaaag ggtattctca
     8281 aagcaggaca agaaataaag aaaaatttca agtctaatga aaaggtatca gcaggtctgg
     8341 tacctttttg tttttattct ctgtccggtt cgtctaatcc tccggttatg tgctgcacta
     8401 tccgccactg attaaaaaag actttggcta ccagctttag agcagtgtaa cttggtattg
     8461 ctatgagcat agcaagaata cctcccgttt tgccggctac cattatgacc aggaatatct
     8521 caagcgggtg tgcgtcaaca ctctttgagt aaacataagg ctgcaccaga aaattatcca
     8581 ccagctgggt cgctccaaaa gtcagtaaaa ccttgatgat cataggcact atctcagtgt
     8641 agaagttcag atcgagattg gacgaaatgg taatcatcac accgaacaac atagctatca
     8701 ctatgcctat gtaaggaatg acattcatca gcgccccaaa aaaagctatc aggagggcat
     8761 tttttatacc aagtatagtc aaggctaccg atatcagcac ggtaaggata ctcacctgta
     8821 gtacgatgcc cccaaaataa ctcctgagca tcgtagatat actgtgcaag gcctttatta
     8881 catggtcttc gtatttctta ggaagcaacg acttgagaaa atcatcaaac atggtctggt
     8941 ctttcagaaa aaagaacaga atgaacatga tggatgttgc cgctatgaca gcattgccaa
     9001 cagcacctac tgccgaagta aaaaaagcac ccacctttga cggctcaata aacttcttaa
     9061 tattggcaag tatctgctgg tcaatgtcaa cggttggcga tatcagcccg tactgtacga
     9121 gccagttctt taactcgttg agcggtatgt gcagggtatt gttcaaggca gatatatcca
     9181 ccttcgatag gttgttggcc tgctctatca caagcggtac gaacaaccag aacaaaattg
     9241 tcagcacaag gaaaaataac aacatcgtca gaaatgctag caaagtattg cccggcttaa
     9301 tttttctgaa tgtaagtatc ttcttcaaaa attcgctcac cggcctgccc agtaacgaaa
     9361 gcacccaggc tatcagcaca taactgacta tatcactcag tttatagaca agataaagcc
     9421 ccagtaaagt tgccccaatt atcagtagtg tccttgcatt tattctcata cgccaggtac
     9481 tttgttttaa aatcaaataa ttataaagat gcccgcagta tctgtacaaa ccgaaataca
     9541 tcaagatagc tgttgtacaa gggcacgggt gctatcctta ccacatcggg ctcgcgccag
     9601 tcggctatta cgccggcttc acttatcttg ccgaacaagg tcttatctgc accctttaca
     9661 cgtatcgaca actgacaacc acgctgcccg ggctcagttg gtgttatcag ctctatcgca
     9721 tcagaatcta tagcctttat caaatattct gtataagccg tcagatttct tgatttt
//
LOCUS       DAOQQY010000076         9612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2640_length_9612_cov_5.654808, whole genome shotgun sequence.
ACCESSION   DAOQQY010000076 DAOQQY010000000
VERSION     DAOQQY010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2640_length_9612_cov_5.654808"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="PLE23_05350"
     CDS             <1..656
                     /locus_tag="PLE23_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX82726.1"
                     /translation="MMKHLNKVDRPMLIEVPKHAVLSKDDFLRAQQNKLTIIDTRTLN
                     DFAKGLNDGVLQINGGKFFSTWLGSVLDYNQQIVLIAPEQNREDLTRKLMRIGMDNIY
                     GFVSPADVPAPAQSDLISMQEFKQYLNDKNVVVLDVRNESEYKSGHIAGVANLAFARI
                     LELEGKHAKNKPIVVHCQSGVRASIAYSMLKKQGFTNVKNYTGGMNEWVDANNAVVQ"
     gene            803..1948
                     /locus_tag="PLE23_05355"
     CDS             803..1948
                     /locus_tag="PLE23_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82727.1"
                     /translation="MKYLLFCLCFFIAGFRMTIAQQTISGSFMHDGKLRTYSFYVPAS
                     YNASKPAPLLINLHGYTSDGIQQALNSGFMPIADTAGFIIAHPDGTKDFLGNRFWNYK
                     ILGVSVDDVGFLERLCDTISAKFNINQNRIYAAGMSNGGYMSYYLACTSDRFAAVGSV
                     TGSMPTVLYDCNQSYGTPVIEMHGTADPTVPYDGNLTSKPIEDVVKYWVQKNKCNPVP
                     TINEVSDIDKTDDATATHFVYTGGTDGHAVEFFKINNGGHTWPGSAFDSPLAGNTCRD
                     FNAAKEIWRFFSQFEKNKGTAVYDRYGANCLSFYPNPATDFLIFKNTELLSSVIQISD
                     VSGRIVSTILKQNTSAVADISTLQPGIYFIKAESHEKVSIQKLVVVK"
     gene            2015..4048
                     /locus_tag="PLE23_05360"
     CDS             2015..4048
                     /locus_tag="PLE23_05360"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HMX82728.1"
                     /translation="MKKTFYSVFMLLLVMVMAACGVKKPENSTMKKTTEMSQPTGSNV
                     VDRFADIQVLRYDINGFDNLSLNQKLLVYYLAEAGYYGRDINYDQNFKYNLEIRKAFE
                     TIVANTKGKDNDENYKKFLTFAKQFWFANGIHHHYGMDKFTPEFSQSYFIELMKSTGA
                     KLSPPAIEAIFNPSMYAKRKVKDATKDMIVSSANNFYGEGVTQKMVEDYYSRIIDKND
                     PEPIEYGLNSTMILKDGKAYEDVWKIGGRYGKSLEKVNENLLQAIKYAENEPQRNALQ
                     KLTEYFKTGALKLWNEYNILWAHATQGDIDYILGFIEVYGDAIGKRATYESVIQINDF
                     EASKQMAVVAANAQWFEDNSSIKPEHKKKKVTGVSYKVVQVAAESGDCSPSTPIGVNL
                     PNNEWIREKHGSKSVSLGNIIAAYEKASGPGMLKEFAYDQAEIDRGTKYAALAGKLHT
                     ALHEVIGHASGQINKGVGQPRETLKNFASTLEEARADLVGLYYLMDQKLVDIGLMPSL
                     EVGKAEYDGYIRNGMLTQLQRLDMGKNLEEEHMQNRQLVASWAFAKGRKDNVVEKVVR
                     DGKTFYHINDYNKLRTIFGDLLREIQRIKSEGDYNAGKALVENYGTKVDPTIHAEVLK
                     RVKPLNLAPYSGFVNPILELVKDKTGKPVNVKITNNQGFVEQMLYYGKKYGTL"
     gene            4651..5022
                     /locus_tag="PLE23_05365"
     CDS             4651..5022
                     /locus_tag="PLE23_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HMX82729.1"
                     /translation="MKPIITNTDYNTLKTLIANCPQHLKTAELGKLNQELDKADIVEE
                     TALDKDVIKINSSFEAEDIATSKKMKLQLTLPEDANINEKKISIFSPLGVAMLGFRKG
                     MTIQWALPGGLKQIRILDVLN"
     gene            complement(5426..5635)
                     /locus_tag="PLE23_05370"
     CDS             complement(5426..5635)
                     /locus_tag="PLE23_05370"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HMX82730.1"
                     /translation="MSIVKVIEVIASSETSFDDAVQKAVAEVSKSIKNIDSVYVKDMK
                     CHVKDGKIASYGVICKVSFRVDPNM"
     gene            complement(5802..6416)
                     /locus_tag="PLE23_05375"
     CDS             complement(5802..6416)
                     /locus_tag="PLE23_05375"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMX82731.1"
                     /translation="MKLKPESEKLDDYLGSVLPFIVFETPLVKSKILEIKSITNNKAL
                     QAKIAFEFVRDNISHSFDTNSSLITIGAEETIEKQEGICFAKSHLLASLLRGSGIPAG
                     FCYQRVLRKATVESGYALHGLNALYLDGTGWFRVDPRGNKAGINSQFSTDEEKLAYPI
                     REELGEVDYPDIFTKPLASVTKSMEESGDCHVLFYNRPEALGQG"
     gene            6701..7153
                     /locus_tag="PLE23_05380"
     CDS             6701..7153
                     /locus_tag="PLE23_05380"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HMX82732.1"
                     /translation="MGLYKFVLLVHVLSAILGLGPGFIMIYVVTRATNMTELRHAYLI
                     RNRLHIFVMVGGSLLLLSGLLMGLMHPYLWQMKWYVLSLVLFLVALAFGPLVLSPRSK
                     PIKALINDHQGEAIPVEYYLLSKKLFLYERIENLIFIAIIILMVLKPF"
     gene            7374..8615
                     /locus_tag="PLE23_05385"
     CDS             7374..8615
                     /locus_tag="PLE23_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMX82733.1"
                     /translation="MLGYRRLIWLIFILVALSFNTVKAERIGLIIAIGQYEKAGNGYT
                     NLSSLRDVILLDSVLKLKKFDKIIILRDEQATKSGIVSAIRSITSSVKRGDVVFIHFS
                     CHGILIADENGRYAEECLVTWDSPFHLKPGTQYKGERHLTYTELKQLRNTLRQKLGRN
                     GDVLMLFDACHSGGLTRGRDRIRGVASSLKIQGWRTFSNQRFGSSPMEDSLDSGSPYV
                     AISACKTEQSNHETSDGQHACGSLSLVFTKAVLALTDKELSYDRLYDRICDEMFRIHP
                     AQFPVMTGTNTARKIFGGQFVPHDDYFRILSYNAKRNEYTMDGGKIHSLTSGSEFDLV
                     NYENDRVVTQNLKMKIIKEPGVLTSIIQPVGELSENFNYRRCKLKLATLAYSGRKITI
                     SLDSLSRTNPALVATLTGYFI"
     gene            8652..9272
                     /locus_tag="PLE23_05390"
     CDS             8652..9272
                     /locus_tag="PLE23_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82734.1"
                     /translation="MYLQTGKNADIIEIRHANDGSLACNAALTPENIRQSLITYAQFT
                     ALRQLNLRNEAFKVAVGLLTIDGKEANNILYSGQTVQMCIENNNESKKSVFVNVFDFM
                     PDGRYKLIIEDLQIDGDSKECFSSTVSEPFGHETIMVMSTPMALSLGSLSPDGRDAVL
                     KSRGTELHILQEIFGWPTTGTIRGKNPKQEEGYTIRLNFNILPNHN"
     gene            9285..>9612
                     /locus_tag="PLE23_05395"
     CDS             9285..>9612
                     /locus_tag="PLE23_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82735.1"
                     /translation="MNTPEFLKALLFYVFLVISALGSTHATTFGDTTKTTIIIVSVCE
                     RPYLDFGKYKVKLRSDTTITVKNLSLFTSALSFPKPGKGYIKFIVKGKKEVLTIYNTE
                     QAKQLVQ"
BASE COUNT         2961 a         1959 c         1923 g         2769 t
ORIGIN      
        1 ccatgatgaa gcacctgaat aaagtggacc gtcctatgct cattgaggta ccaaagcatg
       61 ctgtactgtc taaggatgat tttctgagag ctcaacagaa taagcttact ataatagata
      121 caagaacgct taacgatttt gccaaaggcc tcaacgatgg tgtattgcag attaatggtg
      181 gcaaattttt ctctacctgg cttggttctg tcctcgatta caaccaacaa atagtactca
      241 tcgctccgga gcagaaccgc gaagacctga cacgcaagct gatgcgtata ggcatggaca
      301 atatctatgg gttcgtctct ccggccgatg tacctgcacc ggctcagtca gatcttatca
      361 gcatgcagga atttaaacag tatctcaatg ataaaaatgt tgtagtactg gatgtgcgga
      421 atgagagtga atacaaatcg ggtcacattg ccggtgttgc taacctggca ttcgcaagaa
      481 tacttgagct tgaaggaaag catgccaaaa ataaacccat cgttgtgcac tgccaaagtg
      541 gtgttcgggc ttctatagcc tattcgatgc taaagaaaca agggtttaca aacgttaaaa
      601 attataccgg tggcatgaat gagtgggttg atgccaataa tgcagttgtt caataaaata
      661 ttactcaact tatatagcct gctgatctat attggcaggc ttttttgttt tggtgaaata
      721 agaatatgta aagaaattgc ccgaccgaac cggtattatg tcagaaaatg ttattttcgg
      781 cttctgaaca agttttgtca acatgaaata cttactcttt tgtctatgct tctttatagc
      841 tggttttagg atgaccattg cgcagcaaac cattagtgga tcattcatgc atgatggcaa
      901 attgcgtacc tattcgtttt acgtacctgc ttcatacaat gcctccaaac cggcaccctt
      961 gcttattaat ctccatggct acacctccga tgggatccag caggcgctta attcgggctt
     1021 tatgcccatt gcagatactg ctggttttat cattgcccat cctgatggta ccaaagactt
     1081 tctcggcaac cgtttttgga attataaaat actgggtgtg agtgttgatg atgtgggttt
     1141 tcttgaaaga ttgtgtgata ccatttcggc aaaattcaat attaaccaaa atcgtatcta
     1201 tgctgcagga atgtccaatg gtggttatat gagttactac cttgcttgta caagcgatcg
     1261 ctttgcagct gtaggctctg ttaccggatc aatgcctacc gtattgtatg actgtaacca
     1321 aagctacggg acacctgtga tagaaatgca tggcactgca gacccaaccg taccatacga
     1381 cggcaacctg acatcaaagc cgatagaaga tgttgtaaaa tactgggtgc agaaaaacaa
     1441 gtgcaatcct gtacccacaa taaatgaagt atcggatatt gacaaaacag atgatgctac
     1501 ggctactcat ttcgtttaca caggaggcac tgatggccat gcagtcgaat tttttaagat
     1561 taacaacggt ggtcacacat ggcccggttc agctttcgac tcgcccttgg ccggcaatac
     1621 ctgtcgggat tttaatgcag caaaagaaat atggcgattt tttagccaat tcgagaagaa
     1681 taaaggaaca gcagtttatg accggtatgg tgcaaactgt ctttcttttt atccgaatcc
     1741 ggcaacagat ttcctcattt tcaaaaacac agaactgctc agttcagtta ttcagatatc
     1801 agatgtcagt ggccgtattg taagtactat cctgaaacag aatacatcag ctgtggcaga
     1861 catctcgaca ttacagcctg gcatatattt tatcaaagca gaaagtcacg aaaaagtaag
     1921 tattcaaaag cttgttgtcg taaaatagca gttttccctt tgtttttgtt aaatttgtat
     1981 cttcaaattt ttctaactct ttaaattaat gttcatgaaa aaaacgtttt attctgtatt
     2041 catgctatta ttggtaatgg taatggcagc atgcggagtt aaaaagcccg aaaattcaac
     2101 tatgaaaaag actactgaaa tgagtcagcc taccggtagc aatgtggtgg acagattcgc
     2161 agatatccag gtgttgcgct atgatattaa tggcttcgac aatcttagcc ttaaccaaaa
     2221 actactcgtg tactacctgg ctgaagcagg ttattatggt cgcgatatca attacgacca
     2281 gaatttcaaa tataatctgg aaatcagaaa ggcattcgaa acaatcgtag cgaataccaa
     2341 aggaaaagac aatgacgaaa attacaagaa attccttacg ttcgcaaaac agttttggtt
     2401 tgccaatggt attcatcacc attacggcat ggacaagttt acacctgagt tttcacagtc
     2461 gtacttcatc gaactgatga aaagcactgg ggccaagctg agcccaccgg caattgaagc
     2521 cattttcaac ccttcaatgt acgctaagcg caaggtaaaa gacgctacca aggacatgat
     2581 cgtatcatct gccaataact tttatggaga aggtgttacg caaaaaatgg tagaagatta
     2641 ttatagccgt atcatcgaca agaacgaccc cgaacccatt gaatacggcc tcaattctac
     2701 gatgattctc aaagacggaa aagcctatga agatgtgtgg aaaataggtg gccgctacgg
     2761 caaatcgctt gaaaaggtaa acgaaaacct cttacaagcc ataaaatatg ccgaaaacga
     2821 accgcagcgc aatgctctgc agaaactgac cgaatacttc aaaacaggcg ccctcaaact
     2881 ctggaacgag tacaacatcc tctgggcaca tgccacgcag ggcgatatcg actatatact
     2941 tgggtttata gaagtatatg gtgatgccat aggcaaacga gcaacttacg aaagtgttat
     3001 acagatcaat gatttcgaag catctaagca gatggcagta gtagctgcca atgcccagtg
     3061 gtttgaagat aattcttcta ttaagcctga acacaaaaag aaaaaagtaa ccggagtgag
     3121 ctacaaagtt gtacaggtag ctgccgaatc gggcgattgt tcgccttcta cgcctatagg
     3181 tgtcaatcta cccaacaacg aatggatacg tgaaaaacat ggttcgaaat cagtaagcct
     3241 tggtaacatc atagctgctt acgaaaaagc aagtggccct ggtatgctga aggaattcgc
     3301 ctacgaccag gcagaaatag atcgcggaac caaatatgcg gcactcgccg gtaaactcca
     3361 cacggcactc catgaagtca tcggccatgc atccggccag atcaataaag gtgtcggtca
     3421 gcctcgtgaa acactcaaga acttcgcaag taccctcgaa gaggccagag ctgatcttgt
     3481 aggcctctat tatctgatgg accaaaaact agttgatatc ggtctgatgc cttcactcga
     3541 agtagggaaa gccgaatacg acggctacat ccgcaacgga atgttgacac agctccagcg
     3601 tctcgatatg ggcaaaaacc tcgaagaaga acacatgcaa aaccgccaac tggtagcttc
     3661 atgggcattc gcaaaaggaa gaaaagataa tgtggtagaa aaagtagtga gagatggtaa
     3721 gacgttctac catatcaatg attacaacaa actccgcaca atattcggcg atctgcttcg
     3781 cgaaatccag cgtatcaagt cggagggtga ctacaatgca ggaaaggctt tggtcgaaaa
     3841 ctacggtacc aaagtggatc ctaccatcca tgccgaagta ctcaaacgtg tgaagccact
     3901 taacctggct ccttacagtg gcttcgtcaa ccccattctt gaactcgtca aagacaaaac
     3961 cggcaagccg gtaaatgtca aaattactaa taatcagggc ttcgtagaac agatgcttta
     4021 ttacggtaaa aaatacggaa cgctgtaatc gtacagccga cttgtacaaa tttttacact
     4081 gattaatcgg gcagtttcag atcaatgaaa ttgcccgttt gatattaatt ctatactcaa
     4141 atgccggtat attcctgata ttgccatagg acattaaacg gaattggtaa gtacaaaaaa
     4201 tccataaaat agcgataaaa gcatagtaaa caatgttttt tgagcttttg caatggattt
     4261 gaagtaataa ttatttgaag taaatagtca gaaacgattt gaggtttgta ccaaactcaa
     4321 tcttccttct tgcaccttac cttaccataa atcaaactta aatttgcata tttcacaact
     4381 atttcttaat tttgtggtta tgaagtgcat gaaacaattt caccatcaat caactgcctc
     4441 acgcccgcag tacgtatgcg gtcgattgtt tcgtattgtc agaagcgaca gaagtttcat
     4501 ttcatagacc ctccgttttt cttgttgaat attatttcta agcggagggt gcacattctc
     4561 cggacaatat ttattttctc atttcttaac cttctgcagc tgctcaatac atacagcact
     4621 gtagtcactt tttaaaaaat ttattataca atgaaaccga tcataacgaa tacggattac
     4681 aacacactaa aaactctgat tgctaattgc cctcagcacc ttaagacagc agaactcggc
     4741 aaactcaacc aggaactcga caaggccgat atagtagaag aaacagccct tgacaaagat
     4801 gtcatcaaga ttaattcttc atttgaagct gaagacattg ctaccagcaa gaaaatgaag
     4861 ctccaactta cactacccga agatgccaac atcaacgaaa agaagatatc tatcttttca
     4921 ccactcggtg tggctatgtt gggctttcgc aaagggatga ccatccagtg ggcattgccg
     4981 ggcggcctca aacagatccg catcctggat gtacttaact aatatgtttg tcattcgtac
     5041 tacataaaaa gcattttggc tacacttgtc aaaatacttt tttattcgaa aatttgccgg
     5101 gatggttacc gatgggattg ccagattgaa gaaaaatacc gggtaccgta ctctatcgta
     5161 ctacattccc aatattttgg catttgataa gccggaaata tcaaaagcgg catattaccg
     5221 caatatttga caacaataaa agcttcattg attgacaaaa cattcgcaag tgttaattaa
     5281 tgcatattta gcttgtaaca gtttgttact aaattagctt tatgtttcaa cgcacacatt
     5341 cattaaaaaa agcaccccga cacaatgcct ggggtgcttt tttttgtacg gctatggtaa
     5401 aataacctgt aattgcagtc gaaaattaca tatttgggtc aactctgaaa gacactttac
     5461 agataacgcc gtaactagcg atcttgccgt cttttacgtg gcacttcata tcttttacat
     5521 acacagagtc aatatttttg atggattttg atacctcagc tactgctttt tgtactgcat
     5581 catcaaaact tgtctctgaa gaggcgataa cctcgattac ttttacaatt gacatagatt
     5641 caaattttaa tattaaaaaa ttactttatt atactttatt agacgcccaa gggctgaatt
     5701 ggtaacaaac tttttatacc aataatcaga cagcacatgt attaaacggc ttattttatt
     5761 gagtattata ttttcgggtc agcattatta tcttttgttt attaaccttg tcctaaagct
     5821 tccggtctat tgtagaataa cacatgacaa tcacccgatt cttccataga cttcgttaca
     5881 gatgcaagtg gtttggtaaa aatatcggga tagtccactt cgcccaactc ttctcttatt
     5941 ggatatgcca gtttttcctc atctgtcgaa aattgcgaat tgattccggc tttgtttcct
     6001 ctcggatcta ccctaaacca tcctgtacca tcaaggtaga gtgcattgag accgtgcagt
     6061 gcatagccag attcgactgt agctttgcgc aatacacgct ggtagcaaaa tccggctggt
     6121 atgcctgagc cccgcagcag agaggcgagt agatgtgact tagcgaagca gattccttct
     6181 tgtttctcga tggtctcttc ggcaccgatg gttattaggc tactgttggt atcaaacgaa
     6241 tgtgaaatgt tgtctcttac aaactcaaat gcaatcttag cctgcaaggc tttattattg
     6301 gtaatagatt tgatttccaa tattttgctt tttactaatg gcgtttcgaa cactataaat
     6361 ggcaagacag agccaagata atcatccagt ttttcagatt caggttttag tttcatgata
     6421 ttgtattatc atttgtgtct gtatatatga acaatttttc tgcagttttg tgcacgacat
     6481 ttgatgtttt ttttatctgg aagccaaatt agggaaaact aatttgcagc aagtatattt
     6541 gtatagaccg gcacatcaaa aatacgattc tttaccgtaa atttacgcta tgactgcttt
     6601 gtagctcaag agagaagaag tattgattgt ggagaattac tatatctcag catattgagt
     6661 tgtcaacagc agctatgtaa taaaaactga agaggagaag atgggtttat ataagtttgt
     6721 tttgctcgta catgtacttt ctgccatttt agggcttggc ccgggcttca ttatgatata
     6781 cgttgtcacc cgtgccacca acatgacgga gctcaggcat gcatatctta ttcgcaaccg
     6841 tttgcatatt ttcgtaatgg tgggcggatc cttgctgctg ttatcagggc tgctgatggg
     6901 cctcatgcat ccatatctgt ggcagatgaa atggtatgtg ctcagtcttg tacttttcct
     6961 tgtagcactt gccttcgggc ctttggtttt gtcaccccgc tccaagccga taaaagcatt
     7021 aattaacgac catcaaggag aagccattcc tgttgagtat tacttgctgt caaaaaaatt
     7081 atttctgtac gaaaggattg aaaacttaat tttcattgct atcatcatat tgatggtctt
     7141 gaagccattt tgaagattgg taacctgtca aagaacctca atgaaaatta tgtaagatgt
     7201 cagcaatatt cgttttcgag caaagaatat gttttacatt actttattgc tgttttttta
     7261 ttgtatgctg ttaatattta tgccgatttt gtattaaaca gtaagagaaa gttttattat
     7321 ttttacaata tcggctttcc cttcatttgt gttaaaatat taaacgacta atcatgttgg
     7381 gatacagaag gttgatttgg ttgattttta ttttggttgc acttagcttc aacacagtca
     7441 aagctgagcg gatagggctc atcatagcaa tcggtcagta tgagaaggca ggcaatggtt
     7501 atacaaatct gagttcattg cgtgatgtta tcttgcttga ttctgtatta aagcttaaaa
     7561 aatttgacaa aataatcata ttgcgtgatg agcaggctac caagtctggt attgttagcg
     7621 ccatacgttc cataacttct tctgtcaaac gaggagatgt agtgtttatt catttttcct
     7681 gtcatggcat actcatagca gatgaaaacg ggcgttatgc cgaagagtgc ctcgtaactt
     7741 gggactctcc atttcatcta aaacctggta cacagtataa aggagaaaga cacctcacct
     7801 acaccgagtt aaaacaatta agaaacacac tccgccaaaa actcggacga aacggcgatg
     7861 ttcttatgct gtttgatgca tgtcattccg gaggattaac acgtgggcgc gaccgcatac
     7921 gtggagtagc ttcttctttg aaaatacaag gttggcgaac attttccaat caaaggttcg
     7981 ggtcatctcc aatggaggat tctttagact ccggttcacc ttacgtggct atctctgcct
     8041 gtaaaaccga acaatcaaac catgaaacaa gcgatggcca acatgcttgt ggttctctta
     8101 gccttgtatt taccaaagca gtattggcat tgacagataa agagttgagc tatgacaggt
     8161 tgtacgaccg tatttgtgat gaaatgttca gaattcatcc cgcacagttc cctgtgatga
     8221 caggcaccaa cacagctcgg aaaatatttg gcgggcaatt cgttccacac gatgactatt
     8281 ttcgcattct gtcttataac gcaaaacgca atgaatatac catggatggt ggtaaaattc
     8341 attcactcac atcagggtca gaatttgatc ttgttaacta cgaaaatgac agagttgtaa
     8401 cccaaaacct taagatgaaa atcataaaag aaccgggtgt attgaccagc attattcaac
     8461 ctgttgggga attgtctgaa aactttaatt acagaaggtg taaactcaaa cttgctactc
     8521 tggcttattc aggcagaaaa atcaccatca gccttgattc tttgtctcga accaatcctg
     8581 ctcttgttgc aactttaaca ggatatttta tttaagatca ggaaatcagt gtccgaccct
     8641 cagaaagtga gttgtacctt caaactggta aaaatgccga tattatcgaa ataagacatg
     8701 ctaatgacgg tagtctggct tgcaatgcag cactcacgcc ggagaatatc agacaatctt
     8761 tgattacata cgcacaattc acagccctta ggcaattaaa ccttcggaat gaagctttca
     8821 aggtagctgt tggattgctt accatagatg gcaaggaggc taacaacata ctgtattcgg
     8881 gacagacagt acagatgtgt atagagaaca acaatgaaag caaaaaatca gtattcgtaa
     8941 acgtctttga ttttatgcct gatggacgat acaagctcat cattgaagat ctgcaaatag
     9001 atggagacag taaggaatgt ttttccagca cagtttctga gcctttcggt cacgagacaa
     9061 taatggtcat gagtacacct atggcgctta gccttggcag cttgtcacca gacggcagag
     9121 atgcggtatt gaaatcgaga ggcactgagc tccatattct tcaggaaata tttggttggc
     9181 cgacaaccgg cacaattcga ggcaaaaacc ctaaacaaga agaaggatac accatacgac
     9241 tcaatttcaa tattttaccc aatcataatt aacaaataat atctatgaat acacctgaat
     9301 ttttaaaagc actgctgttt tatgtttttt tggttatttc tgcacttggt tccacgcatg
     9361 caacaacatt tggtgacact actaagacta ccatcattat cgtctctgta tgcgagcgac
     9421 catatctaga ttttggtaaa tataaagtaa aactacggtc cgatactacc attactgtta
     9481 agaacctgag tctttttaca agtgccctat cattccccaa gcccggcaaa ggctatataa
     9541 agtttatcgt caaaggtaaa aaggaagtgc tgactattta caataccgaa caagcaaagc
     9601 agttggtaca ag
//
LOCUS       DAOQQY010000077         9593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2650_length_9593_cov_4.995701, whole genome shotgun sequence.
ACCESSION   DAOQQY010000077 DAOQQY010000000
VERSION     DAOQQY010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9593
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_9593_cov_4.995701"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PLE23_05400"
     CDS             complement(<1..789)
                     /locus_tag="PLE23_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMX82736.1"
                     /translation="MSENQKIIPVNIEEQMKSAYIDYSMSVIVARALPDVRDGLKPVH
                     RRVLFGMSELGMSHTKPYKKSARIVGEVLGKYHPHGDSSVYDAMIRMAQEWSLRYPLV
                     EGQGNFGSVDGDSPAAMRYTEARLQRISDEMLNDLDKETVDFALNFDDSLEEPTVLPT
                     KFPNLLVNGATGIAVGMATNMLPHNLTEVVDGILATMDNPDITTEGLMEHIKGPDFPT
                     GGIIYGIKGVKEAFETGRGRVVVRGRAEIETSEQGREFIIITEIP"
     gene            999..1727
                     /gene="ubiE"
                     /locus_tag="PLE23_05405"
     CDS             999..1727
                     /gene="ubiE"
                     /locus_tag="PLE23_05405"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HMX82737.1"
                     /translation="MDNKVKPYSEHGSKKTQVAEMFDNIASAYDFNNQILSLGIDRIW
                     RNILVSTIEKTNPRYILDVATGTGDVAIRLAKKIHSAKVTGIDLSENMLHLGQNKVMD
                     QKLTDRITLQKGDAEHLDFEDDSFDAITVAFGVRNFETLVKGLSEILRVLKPGGMLGI
                     LEFSKPSNRIFAFFFNLYFKTILPLAGSITSKDNQAYKYLYESVQAFPEGENFLKILE
                     STGYQKSKCRKLSFGICSLYTAYK"
     gene            1676..2407
                     /locus_tag="PLE23_05410"
     CDS             1676..2407
                     /locus_tag="PLE23_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMX82738.1"
                     /translation="MQKIKFWNLQFIYCLQIVLFCLIYTSATAQKFGSNQNYLEFKNK
                     PYYFGITLGYNSSDYKLFRSQNFIGNDSIRLAESVKGPGFNLGIVTNYKINEYFDLRL
                     LPTLSFAERNITYAVPKNRRPPYLQKIESVFFEIPVHLRYTSHPYKDIRAFILGGVKY
                     SVDVASNSRTRQKAELLKVSPSDFSVELGAGIQMFFPYFIFSPEIKFSRGISNILIFD
                     KDLKESNILEKILSSGFTISFHFEG"
     gene            complement(2379..2981)
                     /locus_tag="PLE23_05415"
     CDS             complement(2379..2981)
                     /locus_tag="PLE23_05415"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HMX82739.1"
                     /translation="MNYLAHLVLSGDDPEIMTGNFMGDFLTRSQLKGKTDNFVIGYDL
                     HMFIDKYTDADTDVDAVISLFRQKHGKYAVVIADIIFDFFLCNNWQMFYTTTLDEFMN
                     SVYHKIETQLHLIPEKVSSLIRRMIAGNFIEKYKTLDGLHFIMQKMDQRASFSAGFVE
                     SLSIVKDNELFINDKFLTFYLKLKSATEQQLLILQSGMKS"
     gene            3051..4148
                     /gene="ychF"
                     /locus_tag="PLE23_05420"
     CDS             3051..4148
                     /gene="ychF"
                     /locus_tag="PLE23_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMX82740.1"
                     /translation="MGLKCGIVGLPNVGKSTLFNALTSAKALAANYPFATKEPNLGVI
                     VVPDKRLDELARLIDPQKILPTTIEILDIAGLIKGASKGEGLGNQFLANIREVDAIIH
                     VVRCFANDNVIHVDGSVDPVRDKEIIDLELIFKDLEGIDKRIDRQKKIAKSGSKDDQR
                     IVEVLEYLKSHLESGKPARSFEREDQDEGVFREMMLLTDKPILYVCNVDETSSIKGNE
                     YTERFKESIKDENAEIILISAGIEAEIAELDSLEDRQAFLAEMGLDEPGVNKLIRAAY
                     HLLNLITYITAGVKEVRAWTVTRGTLAPQAAGVIHTDFEKGFIRAEVIKYADFVHYGS
                     EAAVKEAGKMSVEGKEYTVQDGDIMHFRFNV"
     gene            complement(4244..5296)
                     /locus_tag="PLE23_05425"
     CDS             complement(4244..5296)
                     /locus_tag="PLE23_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HMX82741.1"
                     /translation="MALKLLLRDGLREAMIEEMRIDENVFLMGEEVAEYNGAYKVSKG
                     MLEEFGAKRVIDTPIAELGFAGIGVGAAMNGLRPIVEFMTWNFAVLAFDQIVNNAAKT
                     LSQSAGQFKCPIVFRGPSGSAGQLAQQHSQTFESWLGNVPGVKVISCVDPIEAKGLMK
                     AAIRDENPVCVMESEVFYAMELEITDDGKRTYSFGGKKFEADGLMPQDGEDVIVPIGK
                     AAIRRAGTDVTIVTFNKMVFVALEAAELLKAEGISAEVIDLRTIRPLDHDTILESVKK
                     TNRLVIVDESWPMFGVASEIAYQIQKYGFDHLDAPVTRVNSADTNLGYSSVFVEEYLP
                     NAQKVAEAVKNVMYVK"
     gene            complement(5351..6709)
                     /locus_tag="PLE23_05430"
     CDS             complement(5351..6709)
                     /locus_tag="PLE23_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HMX82742.1"
                     /translation="MKIHLIAIGGAVMHNLAIALHNLGHEVTGSDDEINSPSKERLLA
                     RGLLPEKFGWFPEKISLDLDLVILGMHARLDNPELNKAIELGLNVQSFPEYIGKCYAG
                     KTRVAIAGSHGKTTTTSMIAHILKANGRKFDYLIGSNIEGFDTMVSISDAPIAILEAD
                     EYLTSPLDRRSKFLHYHPDIAIITGIAWDHINVFPTFDDYLDTFREFIKTIPDKGHLI
                     YYKNDQNLANMLAEPYDVTDRVAYAEFPHHILPNGDLVLSDTSGKEYPIQFFGKHNLE
                     NFQAAFHVCRLLGLSEGAILQAATSFAGAGKRMELICRNEHFAAYLDFAHAPSKLKST
                     IDAVSARFLDFTKIAIYELHTFSSLNVDFLKEYKNTMKNADVKIVYYSKHTLEMKKMN
                     MIEPEDVIKAFNEEDVHIFSEKEELMHFINQQKMYNTVLLWMSSGNFDGLDVKNISKS
                     FV"
     gene            7009..7326
                     /gene="ytxJ"
                     /locus_tag="PLE23_05435"
     CDS             7009..7326
                     /gene="ytxJ"
                     /locus_tag="PLE23_05435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HMX82743.1"
                     /translation="MDGLEQISKKSFEMPVVIFKHSTRCNISSIAKMRLEEDALTLGS
                     QVHFYYLDLLNHRDISAKIAEKYHVHHESPQILVIKDDECILDASHLDIQADEIEEVI
                     AAC"
     gene            7347..8012
                     /gene="mnmD"
                     /locus_tag="PLE23_05440"
     CDS             7347..8012
                     /gene="mnmD"
                     /locus_tag="PLE23_05440"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HMX82744.1"
                     /translation="MLEIFETKDGSNSLRNTISGDTYHSVHGAMGESQHVFVQNGLMK
                     AFEQKTDLIKILELGFGTGLNALLCLKESIQQQRLVEYHSLEKYPVTEEIYSNLDFHN
                     TAELREFHSEYLQLHTSPWDLGIRLRHHFSFTKYLIDFVDYRPSGQYDVIFHDAFAPD
                     SHPEVWKEDFLTKCYQWLADDGFLITYCAKGSFKRALKDVGFVVESLPGPPGKREITR
                     AFR"
     gene            complement(8073..>9593)
                     /locus_tag="PLE23_05445"
     CDS             complement(8073..>9593)
                     /locus_tag="PLE23_05445"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HMX82745.1"
                     /translation="YTNVDYTIENGKVAFSNTKIDATGSYITDPLGNKALVTGGLVHD
                     HFKKWGYDVKIQSPEFIALNTSVLNNNIYYGKGVGAILMTIGGTFGNTNIYINATTAK
                     GSNLTIPFGSTEQAKASGFVRFVNKNVDTSRTNAEKRKPAESSGLNIDMDLSVTEDAL
                     IQLILDPKAGDYIKGYGKGNIQLAFSSTGEFNMFGDYEIVSGEYLFTLLNLVNKPFNI
                     SGGSTVRWTGDPYEGEVNLTASYRDLYTSPYNFILEYLGADNNAITEARKTTKVDLDL
                     SLSGRLLSPNIKLDINFPNISPIIRNYVDSKLRIVRQDQNEMNRQAMALIVTKSFIPP
                     NNNFQGTQYLTSINTLSELMSNQLSGYITELLSDFIKENGVISGIDLKVGYNVYDAST
                     VNPFSTSELQLRMKNNLFSDRLSINIGGNVGTNASSAVSSQTYFAGDLEVTYSITPDN
                     RLKIRAYQRTEPSLEGGRKNKSGIGISYRREFNNFSEVITDLKSGILKKKDLTKSN"
BASE COUNT         2877 a         1863 c         1849 g         3004 t
ORIGIN      
        1 aggtatttcg gtaataatga taaactctct accttgttca ctggtttcta tttcggcacg
       61 gcctctgacc actacccttc cacgtcctgt ttcgaatgct tctttgacac ctttaatacc
      121 ataaatgatg ccacctgtag gaaaatcggg ccctttgatg tgctccatca gaccttctgt
      181 agtaatatcc ggattatcca ttgttgccaa tatgccatcc acaacttctg ttaggttgtg
      241 cggcagcata ttggtagcca tacctacggc aatacctgtt gcaccattta caagaagatt
      301 agggaacttt gtagggagta cagttggttc ttcgagtgaa tcgtcaaaat tcaatgcaaa
      361 gtcaacagtc tctttgtcca gatcgttgag catttcgtct gatattcgct gtaaccttgc
      421 ttcggtataa cgcatagctg ccggagaatc gccatctacc gagccgaagt tgccctggcc
      481 ttctacgagt gggtatctca gagaccattc ttgtgccata cggatcatag catcatagac
      541 cgaactgtca ccgtgtgggt gatatttacc caacacttca cctactatac gggcagattt
      601 cttataaggt ttggtatgac tcatacccaa ttcactcatc ccgaacaaaa cacggcggtg
      661 taccggtttt agtccatccc tcacgtcggg caatgctctt gctacaatca ccgacattga
      721 ataatcgatg taagcagact tcatttgctc ttctatgttt acgggtataa ttttctgatt
      781 ttctgacata tatgtttaaa gaaattcttt tgtattaaaa attaatgtgc aaatttagaa
      841 aatttttagc agaccaatcc tatttatgac ataatttatt aaccattgtt ttaagctaaa
      901 tgaagatcat ggagctggta aataacattt taatattaaa atgttcttaa ttttgtgccc
      961 gaattcaaaa aaacaaataa cactatacat agtaaggcat ggataacaag gtcaaacctt
     1021 attcagaaca tggatcgaag aaaacacagg tggctgaaat gtttgacaat attgcttctg
     1081 cctatgattt taataaccag attttatccc ttggtattga cagaatatgg agaaatatct
     1141 tagtttccac aatagaaaaa actaatcctc gttacattct cgatgtggcg actggtacag
     1201 gtgacgtggc tatcagattg gcgaaaaaga tacattcagc aaaggttaca ggcattgatt
     1261 tgtctgagaa tatgttacat ttagggcaga ataaagtaat ggatcaaaaa ctgactgacc
     1321 gaattacttt acaaaagggt gatgcggaac atctcgattt tgaggatgat agttttgatg
     1381 caattactgt tgcctttgga gtcaggaatt ttgaaacgct tgtgaaagga ctctcagaaa
     1441 tattgcgtgt gctgaagccc ggaggcatgt taggtatttt ggagttttct aaaccatcaa
     1501 accgtatttt tgcatttttt ttcaatttgt attttaaaac aatacttcca ttagccggta
     1561 gtataacatc taaggacaat caagcgtata aataccttta tgaaagtgtc caggcatttc
     1621 ctgaaggcga aaatttttta aaaatattag aatcaaccgg atatcaaaaa tcgaaatgca
     1681 gaaaattaag ttttggaatc tgcagtttat atactgccta caaatagtat tattttgcct
     1741 gatttataca tcagccactg cccaaaagtt tggcagcaat cagaattacc ttgagtttaa
     1801 aaacaaacct tactattttg gtataacact tggctacaat tcatcagatt acaaattatt
     1861 cagatcgcag aattttatcg gtaatgacag catacgtttg gctgagtcag tcaaaggtcc
     1921 cggatttaac cttggcatag ttaccaatta caaaataaat gagtatttcg acctacgcct
     1981 cttacctact ctttcgtttg ctgaaagaaa catcacctac gctgttccga aaaaccgaag
     2041 gccgccttac cttcagaaaa tagagtccgt attcttcgaa attcccgttc atcttcgcta
     2101 tacgtctcat ccatataaag atatccgtgc atttattcta ggtggtgtta aatattcagt
     2161 agatgttgca agtaattcaa gaacaaggca aaaggcagaa ttacttaaag tgtcaccttc
     2221 agatttttct gtagaacttg gagcaggtat tcagatgttt ttcccgtatt tcattttctc
     2281 gcccgaaatc aaattttcaa gaggcatcag caatattctg atatttgata aggacctgaa
     2341 agaatctaac attctcgaga agatactctc ctccggtttt acgatttcat tccactttga
     2401 aggataagca attgttgttc ggtagctgat ttaagtttta gataaaatgt taaaaacttg
     2461 tcgttgatga atagttcatt gtctttgaca attgatagag attcaacaaa tcctgcagaa
     2521 aagcttgctc tctgatccat tttttgcata ataaagtgca acccatcgag cgttttatat
     2581 ttttcgataa aattacctgc tatcattctg cgaatgagcg aacttacttt ctccggaatt
     2641 aaatgaagtt gtgtttcaat tttatggtaa acgctattca taaattcatc caaagttgta
     2701 gtgtaaaaca tttgccagtt attgcaaaga aaaaaatcga aaataatatc tgctattacg
     2761 actgcatatt ttccatgctt ttgccgaaat aaactgataa ctgcatctac atcagtatcg
     2821 gcatccgtat atttatcaat aaacatgtgc aagtcataac cgatgacgaa attatcagtt
     2881 tttcccttaa gctggcttct tgtaaggaaa tcccccataa aattgccggt cattatttcc
     2941 ggatcatcac ctgagagtac cagatgcgct aaataattca aattgcaaat taagtttgtt
     3001 tcacaaaatt aataaatcta actttgcgcc gttttaaaaa aaatcaaatt atgggattaa
     3061 aatgtggaat cgtcggactg cctaatgttg gtaagtcaac tttgttcaat gcacttacat
     3121 ctgccaaagc tttggctgcc aattatcctt ttgctaccaa ggagcctaac ctgggcgtca
     3181 ttgtcgtacc ggataaaaga cttgacgaac ttgcaagatt aattgatcct caaaagattc
     3241 tacctacaac cattgagata cttgacattg ccgggctcat caaaggtgcg agcaaaggag
     3301 aaggacttgg caatcagttt cttgccaata ttagggaagt agatgccatc attcatgttg
     3361 tgagatgttt tgccaatgac aacgtcatac acgttgacgg aagtgttgat ccggtccgtg
     3421 ataaagaaat cattgacctt gagcttattt ttaaagatct ggaaggtatt gataaaagaa
     3481 ttgacagaca gaaaaaaata gcaaaaagtg gtagcaaaga cgaccaacgc attgtagaag
     3541 ttctcgagta cttaaagtcg catcttgaat cgggtaaacc ggctcgttca tttgagagag
     3601 aagatcagga cgaaggtgta ttccgagaaa tgatgttact aacagacaaa ccgatattgt
     3661 acgtatgtaa tgttgatgag acatcttcta tcaaaggaaa tgaatatacg gagcgattca
     3721 aagagagcat caaggacgaa aatgctgaga ttatcttaat atcggctggt atcgaagctg
     3781 aaatagcaga actcgattcg ctcgaagacc gacaagcatt tcttgcagaa atgggtctag
     3841 acgaacctgg tgtcaacaaa cttataagag cagcctatca tttgttgaat cttatcacgt
     3901 acatcacagc aggtgtaaaa gaggtaagag cctggacagt aacccgtggt actttggcgc
     3961 ctcaggcagc cggagtgatt catacagatt ttgagaaagg ctttatccgt gcagaagtca
     4021 tcaagtacgc cgattttgtc cattatggtt cagaagctgc tgtaaaagaa gctggcaaaa
     4081 tgtcggtaga aggtaaagaa tatactgtgc aggatggaga tatcatgcac ttcagattta
     4141 atgtgtaaaa atgaagattc ccagactttt tatttcaaca aaactgttta tagcctgttg
     4201 aaatactgag gccgggaatc acctttttat attctacaga atattatttt acgtacatta
     4261 cattttttac agcctcagct actttttgtg catttggcaa atattcctca acaaaaacag
     4321 aagaataacc gagatttgtg tctgccgaat taacccttgt aaccggagca tccagatggt
     4381 cgaatccgta cttttgtatt tggtaagcta tttctgaagc taccccaaac atcggccatg
     4441 actcatcaac gattacgagc ctattggttt ttttaaccga ttcgaggatt gtatcgtggt
     4501 ctagtggtct gatggttcgc aggtcgataa cttcggcact tataccttct gctttaagta
     4561 attcggctgc ttcgagtgcc acaaatacca ttttattgaa ggtaacaatg gtgacatctg
     4621 tacctgctct tcttatagca gcttttccga ttggtactat cacgtcttca ccatcttgtg
     4681 gcataagtcc gtctgcttca aatttctttc caccaaagct gtaggtacgt tttccgtcat
     4741 ctgttatttc aagttccata gcataaaaga cctcagactc cattacacat accggatttt
     4801 cgtcacgtat ggcagctttc atcaatcctt tggcttcaat cgggtcaacg caagatatta
     4861 ctttgacacc aggtacatta ccaagccagc tttcgaaggt ttggctgtgt tgctgtgcga
     4921 gctgtcctgc agagcctgat ggacctctga acacaatcgg gcatttgaac tgaccagctg
     4981 actgtgataa ggttttggct gcattattta cgatttggtc aaatgcaagg acggcaaaat
     5041 tccaggtcat gaattctaca attggtctta gtccgttcat agcagcacct accccaatac
     5101 ctgcaaagcc aagctcggca atcggcgtgt caatcactct ttttgcacca aattcttcaa
     5161 gcataccttt acttaccttg taagcaccat tgtattcggc gacctcctcg cccatcagaa
     5221 aaacgttttc gtctatgcgc atttcttcaa tcattgcttc gcgcagtccg tctctgagta
     5281 atagttttaa agccattatt tttcaaaatt ttcccaaagt tatataaata taatgtttga
     5341 atgaaatctc tcacacaaaa gatttagata tatttttaac atctagtccg tcaaaattac
     5401 cggaagacat ccaaagtaaa acagtattat acattttttg ttgattaatg aagtgcataa
     5461 gttcttcttt ttcagaaaat atatgtacgt cttcttcatt aaaagcttta attacatctt
     5521 caggttcgat catgttcatt tttttcattt caagtgtatg tttgctataa taaacgatct
     5581 ttacatcggc gtttttcatt gtgtttttat actctttcag aaaatcaaca ttcaggctgc
     5641 taaatgtatg caattcgtaa atggcaattt ttgtaaaatc cagaaatcgg gcactgactg
     5701 catcgatagt gctttttaat ttggatggtg cgtgcgcaaa atcgagatac gctgcaaaat
     5761 gttcgttgcg gcagatgagt tccatacgtt tacctgctcc agcaaaactt gtggcagctt
     5821 gtaatattgc tccttcactt aacccgagca gacggcaaac atgaaaggca gcctgaaaat
     5881 tttctaaatt gtgtttcccg aaaaactgta tcgggtattc tttccccgag gtatctgaca
     5941 aaactaaatc accatttggt aaaatatgat gaggaaattc agcgtaggca acacggtcag
     6001 ttacatcata aggttctgct agcatgttgg ctaagttttg gtcatttttg taatagatca
     6061 ggtgaccttt atctggaatg gttttaataa attcacggaa agtatctaga tagtcatcaa
     6121 aagtcggaaa aacattgata tgatcccagg ctatgccggt aatgatggca atatcaggat
     6181 gataatgaag aaattttgag cgcctgtcaa gcggcgaagt aaggtattcg tcggcttcta
     6241 aaatggcaat aggagcatcc gaaatactca ccatagtgtc aaagccttca atattactac
     6301 cgatgaggta atcaaatttg cggccattcg ctttgagaat atgggcaatc atggatgtcg
     6361 tagtcgtttt accgtggcta ccggcaatgg ctacacgtgt tttcccggca taacatttac
     6421 ctatgtactc cggaaagctt tgaacattaa gcccgagttc aattgccttg ttgagctcag
     6481 gattatcaag cctggcatgc ataccgagaa ttacgagatc aagatcaaga gaaatttttt
     6541 ccggaaacca gccgaatttt tcaggtaaaa gccccctggc cagcaatctt tctttggaag
     6601 gcgaattgat ttcatcatcc gaaccggtaa cttcgtgacc cagattatgc agtgcaatcg
     6661 ctagattatg catgacagca ccaccaattg caatcaaatg aattttcata ataggaaaat
     6721 tgttaaatta atattatttt tgcaaccgga ttcgatcata ttgtttaagt agtataatac
     6781 aatccataaa aattgcgttg tagatttaaa ttaattcaaa tttcatgaaa aacaaatcat
     6841 ttaaaagatt tctgactttg gcgactgttg cagtattctt ccttacattc acttcttgca
     6901 accgaggtac cggatgtccg aataattttt cactaaaatc attggcaaaa gtagtttcta
     6961 atttttaatt tgagtatgga aacttttccc tggcaccaac tttcgagtat ggacgggctg
     7021 gagcaaattt caaaaaagtc atttgaaatg ccagttgtca tctttaaaca cagcacacga
     7081 tgcaatatca gttctatagc gaaaatgcga ttagaagaag atgcattgac acttggtagt
     7141 caggtacact tttattacct cgatttgctc aatcaccgcg atatctctgc gaagatcgct
     7201 gaaaaatatc acgtccacca tgaatcacca caaatcctcg tcatcaagga tgatgaatgt
     7261 attcttgatg caagtcatct tgatattcag gcagatgaga ttgaagaggt aattgcggct
     7321 tgttgaaccc caaatctaag tccggtttgt tagaaatttt cgaaacaaaa gacggcagta
     7381 acagtcttag aaatactatt agtggcgaca cttatcactc tgtacatggt gctatgggtg
     7441 aaagtcagca tgtgtttgta caaaatggct taatgaaagc tttcgagcaa aagactgatt
     7501 tgattaaaat tcttgaactt ggattcggta cagggttaaa tgcgttgctc tgcctcaagg
     7561 aatctatcca acaacaacga ctagttgagt atcattctct tgagaaatat ccggtcacag
     7621 aagaaatata ctccaatctt gattttcaca acacagcaga acttagggag tttcattcgg
     7681 aatatcttca actgcacacc tccccatggg atttgggcat cagactaaga catcatttca
     7741 gctttacaaa atacctgata gattttgttg attatcgacc atccggtcaa tatgatgtca
     7801 ttttccatga tgcttttgca ccagattcgc atcctgaagt ttggaaagaa gattttctta
     7861 ctaaatgcta tcagtggctg gcagatgatg gttttttgat tacatattgt gccaaaggtt
     7921 cgttcaaacg tgctttgaag gatgttgggt tcgttgtaga atcattaccg gggccgccag
     7981 gaaaacgcga aataacaaga gcttttcgtt gattgctttc aatcctgacg gtgtgtgatg
     8041 attgattttt tttaggaaat gtaaattcaa gctcagtttg actttgtcag atcctttttc
     8101 ttcagaatac cacttttcag atctgttatc acttcagaga aattattaaa ttcacgtcgg
     8161 taactaatac cgatacctga tttatttttt ctacctccct ccaacgaagg ttcagtccgt
     8221 tgatatgctc ttattttcaa tctattgtcg ggtgtaatgg agtacgttac ttccaggtca
     8281 ccggcaaaat aagtttgtga acttacagct gaacttgcat tggtaccaac attaccaccg
     8341 atattgattg acaagcggtc tgagaaaaga ttgtttttca tacgcaactg caattcgctt
     8401 gtagaaaatg ggtttacggt agatgcatca tagacattat agccaacctt aagatcaatg
     8461 ccggagatga caccattttc tttaataaaa tcggatagta gctcagtaat gtacccagac
     8521 agctgattcg acatcaattc agacaaggta ttaatagatg taagatactg tgtaccctgg
     8581 aagttattat ttggcggaat gaatgatttg gtaactatta gtgccattgc ctggcgattc
     8641 atttcgttct gatcttgtct tactatgcgc aatttcgaat caacataatt gcggatgatg
     8701 ggtgaaatat ttggaaagtt gatatcgagt ttgatattgg ggcttagcaa tcggccagaa
     8761 agcgacaagt cgaggtcaac tttagtggtt tttcttgcct cagtaatagc attattgtct
     8821 gcaccaaggt attcgagtat aaaattatat ggagaagtat aaagatctct gtatgaagcg
     8881 gtaagattta cttcgccttc ataaggatca cctgtccagc gaactgtact gccccctgat
     8941 atattgaatg gtttgtttac aaggttaagt aaagtaaaaa gatattcgcc tgacactatt
     9001 tcataatcgc caaacatgtt aaactcacct gttgaagaaa atgccaattg gatattgcct
     9061 ttgccataac ctttgatgta gtcacctgct ttcggatcga gaatgagctg tatcaaagca
     9121 tcttcggtca cggacaaatc catgtcgata tttaagcctg agctttcagc aggttttcgt
     9181 ttttctgcat tggtacgtga ggtatccaca tttttattga caaatctaac gaagccagaa
     9241 gctttggctt gttctgttga gccaaatggt atggtaaggt tgctaccttt tgctgtggta
     9301 gcatttatat atatattggt attaccgaat gtgccgccta tggtcattag aattgctccc
     9361 acaccttttc catagtaaat gttgttattc agcacacttg tattgagggc gataaactcc
     9421 ggcgactgaa tttttacatc atacccccat ttcttgaaat ggtcatgaac caaaccaccc
     9481 gttacgagcg ctttattgcc aagcgggtcg gtgatataac ttccggtagc atcaattttg
     9541 gtattggaga aggccacttt gccgttctca attgtatagt caacatttgt ata
//
LOCUS       DAOQQY010000078         9586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2656_length_9586_cov_7.103452, whole genome shotgun sequence.
ACCESSION   DAOQQY010000078 DAOQQY010000000
VERSION     DAOQQY010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2656_length_9586_cov_7.103452"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1535
                     /locus_tag="PLE23_05450"
     CDS             <1..1535
                     /locus_tag="PLE23_05450"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HMX82746.1"
                     /translation="SILAEIVRHAREECKVVIMCSDVNSVKNYLDLVKVDYSSNVDFV
                     VAQANSVWIRDYGANPIYLNRVDSLAFVDWIYNRPRPLDNKMPDVEGTYFNLPVYATT
                     SQPFDLVHTGGNFMSDGMGTGFSSNLVLDENGPNNNYGFSNHSEQEVDSIMYKYMGID
                     KYIKMTNLPFDAIHHIDMHMKLLDEQTLIVGKYPDNIADGPQIEANLQYVLNNFKTRI
                     GTPFRVIRVPMPADKNYLYPDQGGDYRTFANALIINKSVLLPIYEMKYDTTAIKIWES
                     AMPGYKIRGINCDGMISASGAIHCITKEVGVAQPLLLQHFSLDDVQGAYPAGYPIAAH
                     AEHKTGINNVYLNYRIKDKDTTWTRIPMTAFNAELTDYESAIPEQANGTVIEYYIEAT
                     ANNGKIQYKPMTAPLGFYTFKVNNTSGSKDLTRQNLLGEIFPNPAKSITCIPVSCAEA
                     QQLDISIIDVFGKPVKQIFNGKQIQGESKYFFDASQLVSGNYFVKIIGEHGSEVKLVL
                     VQ"
     gene            complement(1589..4813)
                     /locus_tag="PLE23_05455"
     CDS             complement(1589..4813)
                     /locus_tag="PLE23_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82747.1"
                     /translation="MAAIFRFTIQTILLFTLCLSGIRAQVPNCVKCCCGNINEVGIPC
                     GNFQEPPFADQTGWIDYSAGQEYCGWTVTAGTISIHGPYHNNLGAGNPNGASQHMDLN
                     GSTNGTVSTNLTGMTPGYKYTIVLWYAKHNATSVANATIKVAGGTWLNESWSSTNTGS
                     NVWLEKCFSFVAQASTATLEFEGTSPVPCCGMLIDDLTMYECSNDDEVPVFSDIPDAL
                     IEVECPSEIPPAPILTATDNCDAQPTLNYSESQQGTPCKLTITRTWTVSDACDNSATL
                     QQIVNVEDKEAPQFTTLPQDKTVNCDDDLYNEFYNWLGNNGGADASDNCTAINWSKTF
                     NKEPDEACEEIFVTFTIKDLCDNETQAIAKFIVTDTDPPYFTKNPQNTILSCEPDALN
                     QFQNWLANNGFATATDKCGQVIWSNDFDGDFNKTKFDISFTAKDLCGNEITKSATFEI
                     NENSKTTLLFKTTCDKSMSGKRDTVVFSNGSCDSLVITESVFAKSDTSYITGMTCKLS
                     EAVNDTLFLSNQNGCDSLIITSIIYNKPDTTYSIFNICGLQNEYNDTLHFAGQFCDST
                     VILQYKSLPVNVISINNSTCDPSKAGTEVLTLKNQFGCDSIVTITTILSEAIYTYLTK
                     HSCGTGPVFKDTVIYVTAQCDSLVITEHILHPPDATFSSATSCDKKQAGVFTTTFKNI
                     WGCDSVHVLTVNLNPSDTVYITGTTCDKSKEGNFTENLKNQFGCDSIIYRTITYIPSD
                     TTYITELTCDPTQTGTFVNIYPTQQCDSVVIITRNLTQSYIQYFTATTCNSAMAGIDT
                     LKYLSKQGCDSLIITETIFKGFSASVLTVNESCLGFGDGALTIEGLVNAITPVQYSLD
                     KVNWVNDSIFTSLTPGEYTVYIKDAVGCELELTGNKIKAGDTFTIDLGPDITVTSGKR
                     AFLDLSTSSVPASIVWSLDTLNNCIICTKVDFKPSQSTTLFATATSQSGCVAVDSIHI
                     ILLPDIKIYAPNAFSPEVNGINDKWIIYGDQHLANIKELHIYDRWGEQVFYGENIQPG
                     DSTRGWDGKHRGEFMKPAVFVFWAIVEFDDGRPELIKGDINLVR"
     gene            complement(5024..5398)
                     /gene="ssrA"
                     /locus_tag="PLE23_05460"
     tmRNA           complement(5024..5398)
                     /gene="ssrA"
                     /locus_tag="PLE23_05460"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            5812..8712
                     /locus_tag="PLE23_05465"
     CDS             5812..8712
                     /locus_tag="PLE23_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82748.1"
                     /translation="MTRILLELSKNGYMLLFLLLIGLVTSSTHAQTCPGGLGPVVNVA
                     SLPYTGTNLTTCGAGNELTSANTITCGSSSYLGGEDLVFIFTPTATGLTTISLTSGSS
                     WIGLKLYAGCPFIGQGGTCVSNVESSAGNQSMNAGVTAGVTYYLVVDTWPTPNCIPSF
                     NLTISAPTPPPSCPSGLGTYNNITLPYSGTGLTTCGKGNTLTSSNVSPICGSTYYYGG
                     EDDVYIFTPTSSGAYTIALTSSSSYVGIMLYAGCPLAGNGATCVGYSQSSGGNQTINA
                     TLTAGVTYYLIVDTWPSPTCIPSFNLNISGGAPGPCDNIVSLPCGTSATATMSGTGSL
                     ASSSACGWSTPGQEKIYSFTPTASGNATLVVSSTNSAGYVDYQYKAASGGCNGTGWTC
                     IIDLFSPGSATFPVTAGVTYYLLLDPETTSSVTHTFQVNCPSLGSVITMNCPAAVSVS
                     CASQVPPPNPGSVIASTTCSPTTVTVIHLSDVISAQTCANRYTITRTYRASDACGTTK
                     TCTQTITVNDQTPPVVNCPANVNVKCAAQVPVVNLNQVTGADNCTGSVTFSYLGEVIS
                     DQLCDNQYKITRTFRGTDACGNSATCAQVIEVKDDVKPVLGTIPADVTLSCTDPIPAP
                     PVVTGTDNCAGSVSVTFKETSTQSDYPTLCRAYSYEITRVWTAKDACKNTSFKVQKIT
                     VQDTKAPEFTYTPPQFITVECDDDGSNNEDPIAVDDCDGNPSVILNVKYKFGLEGCAG
                     NYIAAYTWTAGDKCGNTSQYTQYITVIDSEAPQIKCPNNVEVSSPIPIAVTWQSPKGS
                     DYCDGPIIPVQIQGPPPGSIFPAPSKTIIVYQATDNCGNVSTCSFLVIVRTGNEGYVT
                     GIGSETSLNNAVQSSQSDAATYLGMTTLYQNEPNPFGNNTTIRFDLETAGTAVIQIMD
                     ITGKTIRQIQGEYPSGKNFINIDNSQLPASGMYYYQLRTKDFNDTKKMLYIK"
     gene            9037..>9586
                     /locus_tag="PLE23_05470"
     CDS             9037..>9586
                     /locus_tag="PLE23_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82749.1"
                     /translation="MTITTCAFAGEYSTITSAVAGSTYQFVATGGAGNYITIRQGSPS
                     GTVIGYGNSPLNVTCTVSGSLYMHLNTNSSCGTDASCHTETIQCTSCGSGGSGCPSGL
                     GSYVNITLPYNGTGLTNCGKGNDITSINVSPICGSSSYYGGEDEVYIFTPASTASYTV
                     SFTSSSSWVGLMLYAGCPLTGGG"
BASE COUNT         2813 a         1901 c         1985 g         2887 t
ORIGIN      
        1 attccatcct ggctgaaata gtgcgtcatg cacgtgaaga gtgtaaagtg gtgataatgt
       61 gtagcgatgt gaacagtgta aaaaattatc ttgaccttgt taaggttgac tattcgagca
      121 atgtagattt tgtggtagca caagccaatt ctgtctggat acgcgactat ggagctaacc
      181 ctatttatct gaatcgtgtg gatagcctcg catttgttga ctggatttat aaccgacctc
      241 gtccgctcga taataagatg ccggatgtgg aaggtactta ctttaatctg cccgtgtatg
      301 ctactaccag ccaacctttt gatctggtac atacaggagg caattttatg tcagatggta
      361 tgggcaccgg tttttcgtcc aatctcgtac tagatgaaaa tggcccaaac aacaattacg
      421 gattcagcaa ccattcagaa caggaagtgg acagcattat gtataagtat atgggaattg
      481 acaaatatat aaaaatgacc aatctgccat ttgatgctat tcaccacata gatatgcaca
      541 tgaaactcct cgacgagcag accttgatcg taggcaaata ccctgacaat atagctgatg
      601 gcccacaaat agaagcaaac ctccaatacg tactcaataa tttcaaaaca cgtatcggta
      661 caccattcag agttatccgt gttccgatgc cggctgacaa aaactatctc tatccggacc
      721 agggtgggga ctaccgcaca tttgccaatg ctttaatcat caataagtcg gtactattgc
      781 cgatatatga aatgaaatat gatacgactg ccataaaaat ttgggaatca gctatgcctg
      841 gctataaaat acgcggaatc aactgcgatg gaatgatcag cgccagtggt gccatacatt
      901 gtattacaaa ggaagtcggg gtagcacaac cactgctact tcagcatttc tctcttgatg
      961 atgtgcaagg agcatatcct gccggttatc ccatagcagc acatgccgaa cacaagactg
     1021 gtatcaataa tgtttacctc aattacagga ttaaagataa ggacaccact tggacccgca
     1081 taccaatgac agcctttaat gcagaactca cagactacga gtcagccatt cctgaacaag
     1141 ctaatggtac tgtgatagaa tattacattg aggctacagc caataatggt aaaattcagt
     1201 acaagcctat gactgcacct ctaggttttt atacatttaa ggtaaacaat acatctggtt
     1261 ctaaagactt aacaagacag aatcttcttg gtgaaatatt tcctaatccg gctaagagta
     1321 ttacgtgcat acctgtgtct tgtgcagaag cacaacaact tgatatcagc atcattgatg
     1381 tatttggcaa accggtgaaa caaatattca atggcaaaca gatacagggc gaatccaaat
     1441 atttctttga tgcttcgcaa ttggtgtccg gtaattattt tgttaaaata atcggtgaac
     1501 acggtagcga agtgaaactg gtcttagttc agtgaatttt gtgatgtagc agaatattaa
     1561 tattcgagaa atttagctca aaaactgcct atcttaccag attgatatcc cctttgatga
     1621 gttccggcct accatcatcg aattcgacaa ttgcccaaaa aacgaatact gccggtttca
     1681 taaattcgcc acgatgtttg ccatcccagc cacgtgttga atcacccggt tgaatgtttt
     1741 caccgtaaaa cacttgttcg ccccaacgat cgtaaatatg taattctttg atgttagcca
     1801 aatgttgatc gccatatatg atccatttgt cattgatgcc attcacttcc ggcgaaaatg
     1861 cattcggtgc ataaatcttt atatcaggta gcaaaatgat atggatggaa tctacagcaa
     1921 cacaccctga ctgactggtg gcagtagcaa ataaagttgt tgactggctt ggtttaaaat
     1981 caactttggt gcaaatgatg cagttattga gcgtgtcaag tgaccacaca atgctagcag
     2041 gtacggacga tgtgctcagg tcaaggaatg ccctctttcc tgatgtgacg gttatgtcag
     2101 ggccaaggtc gatggtgaat gtgtctcctg cttttatttt atttcctgtc aattctaact
     2161 cacagccaac ggcatcttta atataaactg tgtattcacc gggcgtaagt gatgtgaaga
     2221 ttgagtcatt cacccaatta accttatcca gactatattg tacgggcgtt atagcattta
     2281 caagaccttc aatagtcaaa gcaccatccc caaaacccaa acagctttca ttcactgtca
     2341 aaacggaggc gctaaatcct ttaaaaattg tctctgtaat aatgagcgaa tcgcaacctt
     2401 gtttacttaa atactttaat gtgtcaatgc cggccatcgc cgaattacag gttgttgctg
     2461 tgaaatactg gatgtaactc tgggtgagat tccgggtaat aattacaaca ctatcgcatt
     2521 gctgggtcgg ataaatattg acgaaagtcc ccgtttgtgt agggtcacag gtcagctcag
     2581 ttatgtaagt agtatccgat ggaatatagg taatcgttct gtaaataata ctgtcgcagc
     2641 caaactgatt cttgagattt tcggtaaaat taccctcttt tgacttatcg caagtggtac
     2701 ctgttatgta aacagtatca ctcgggttta agttgacagt tagtacatgt acactgtcgc
     2761 agccccagat atttttgaat gttgtagtga acacacctgc ctgttttttg tcacaagaag
     2821 tggctgaaga aaaagtggca tcgggtggat gcagaatgtg ttctgtgatg acgaggctgt
     2881 cacattgggc tgtaacatat ataactgtat ctttgaaaac aggtccggtt ccacaggaat
     2941 gttttgtcag atatgtataa atagcctcgc tgagtatagt agtaatagtg acgatactgt
     3001 cacatccgaa ttggtttttt agcgttagaa cttctgttcc ggctttggac ggatcacatg
     3061 tactgttgtt gatgctaatt acgtttaccg gtaatgattt atactgaaga atgacggtgc
     3121 tgtcacaaaa ttgtccggca aaatgcaggg tgtcattata ttcattctgg agcccacaaa
     3181 tgttgaagat actatatgta gtatccggtt tgttgtaaat aatactcgtt attatcaggc
     3241 tatcgcaacc attctgatta cttaggaata atgtatcatt tacagcttca gaaagtttgc
     3301 aggtcatacc tgtaatgtag cttgtatcag attttgcaaa aacactttca gtaataacaa
     3361 gtgagtcgca tgagccgttt gaaaacacaa ctgtatctcg ctttcctgac attgacttat
     3421 cacaagtagt tttgaataac aaggtagtct tgctgttttc atttatttca aatgtagctg
     3481 atttggttat ttcattaccg cacaaatcct tcgcagtaaa cgaaatgtca aacttagttt
     3541 tgttaaaatc gccatcaaaa tcatttgacc aaattacttg tccgcattta tcagtagctg
     3601 tggcaaatcc gttattagcc agccagtttt ggaactgatt gagggcatct ggttcacaac
     3661 tgagtatcgt gttttgtgga ttcttcgtga aatatggcgg gtcagtatct gtcacaataa
     3721 attttgcgat agcttgtgtc tcattatcac aaagatcttt gatggtgaat gtgacaaaga
     3781 tttcttcaca ggcttcatcc ggttctttgt taaaggtctt actccagttt atagccgtac
     3841 aattatcgga ggcatcagca ccgccattgt tgcccagcca gttgtaaaat tcgttataca
     3901 agtcatcatc acagtttaca gttttgtcct gaggtagggt agtgaactgg ggcgcttcct
     3961 tgtcttctac atttactatc tgttgaagtg tagcggaatt gtcgcatgcg tcggagacag
     4021 tccaggtacg ggtaatggta agcttacatg gtgtgccttg ctgcgattct gaataattaa
     4081 gtgttggttg ggcatcgcag ttgtcagtgg ccgtcagtat tggtgccgga ggtatttcag
     4141 aagggcattc tacttcaatg agtgcatccg gaatatctga aaaaaccgga acttcgtcat
     4201 cattcgagca ttcgtacatg gtgagatcat ctatcaacat accacagcat ggcaccgggc
     4261 tggttccttc aaattcaagt gttgctgtac ttgcctgtgc cacaaaactg aagcatttct
     4321 ctagccaaac attgcttcct gtattggtag aagaccagga ttcattcagc catgtgccac
     4381 ctgctacttt tatagtggca ttcgctacac tagtagcatt atgtttggca taccaaagta
     4441 caattgtgta tttgtatccg ggagtcatac ctgtaagatt agtggatacg gtaccgttgg
     4501 ttgaaccatt caagtccatg tgctgactag caccgtttgg gttgcctgca cccagattat
     4561 tgtggtacgg tccatgaata cttatggtgc cggccgttac tgtccagcca caatattctt
     4621 gaccagccga ataatcaatc cacccggtct ggtcagcaaa tggtggttcc tgaaaattac
     4681 cacacggaat accaacttcg ttaatattgc cacaacagca ttttacacaa tttggtacct
     4741 gagcccgaat acctgaaaga cataaagtaa acaaaagtat tgtctgtatc gtgaatctaa
     4801 atatagcagc catagcaatt gtagcaataa tcttgattaa aaattttcag accaataaat
     4861 cttaaagtaa aattttgtta ggaacaaaga tgcaatatta caaaattctt attagcaaaa
     4921 aatgaaaata agcacttgga ctcggacaag ctgaattttt tgttcaggca gggcaagaaa
     4981 atacatttaa caaaaaaccc gactcaagta gccgggtatt gatttgtgga gctggaggga
     5041 ttcgaaccct cgtccagaga aagtaccaaa gagctttcta catgcttagc ttatgattta
     5101 attgtcggga ccataccaag gctcagaagc aggccttcag taggtcctta ggtcattaat
     5161 ttcaggaaac aatctgacca tgttatttcc caatcctgaa gggtctgatg tgcagcgcgt
     5221 tgtgtatcag gcatcaaacg agggcacaaa ggcactttaa ttcctagaat taagcagcca
     5281 aagcgaagtt attttcgcca attaaaaatt gtcgataatc atttgttgac ggagtgaact
     5341 accatgctcc ggcatgctta ctaatcagtc cgcttacctg tcgattccgg tcagccccta
     5401 ttagtataac ttctttctaa caaacttgct aatatattag ttccaatttt gatcatattt
     5461 tgtttattga tatgtaaaat attttgcatt aaagaatgat ctatataggt taacctttgt
     5521 gatctgttca aaaataccta ctcgtcaact tcatttattc cgttgtacat tttttaaatt
     5581 taagtaattc aatacctttt attattttta ataacaaaat ttttagtatg ttttgatgaa
     5641 aaaaaatttg gtattcagaa taaaatattg tgaaagtaaa ctctaatgaa aatttaatca
     5701 aaattaattg tttaactgaa ttttgtgtgt atatttgtat gatttataaa ttaaatgttt
     5761 gctgttattt gtaataatat ttattttttc accaactatt aaatttaaac catgacgaga
     5821 attttacttg aattgagcaa aaatggatac atgcttttat ttttgctttt aattggattg
     5881 gtcacaagtt ctactcacgc ccagacgtgt ccgggtgggt taggtccggt ggtcaatgtt
     5941 gcatccttgc cttatacagg caccaatctt actacttgtg gagccggtaa tgagttgacc
     6001 agtgccaaca caataacctg tggaagtagt tcttatttgg ggggagaaga tttggtattc
     6061 atttttacac caacggctac cggtcttact acgatttcac ttaccagtgg ttcttcgtgg
     6121 atagggctta aactttatgc aggttgtccg ttcataggac aaggtggaac ttgtgtgtcc
     6181 aatgttgaaa gttctgccgg taaccaatct atgaatgccg gggttactgc cggagttaca
     6241 tattacttgg tggtagatac ctggccgaca ccaaactgta ttccgagttt taaccttacc
     6301 atcagtgctc cgacaccacc accatcatgc ccgagcggtt taggtacgta caacaatatt
     6361 accttacctt attctggtac aggccttact acttgtggaa aaggtaatac gcttacaagt
     6421 tctaacgtga gtcctatatg tggctctact tattattatg gaggagagga cgacgtatat
     6481 atatttacac ctacatcatc aggtgcttat accattgcat tgacgagcag tagttcttac
     6541 gttggcatta tgttgtatgc cggttgtcca ttagctggca atggtgctac atgtgtagga
     6601 tattctcaaa gcagcggagg caatcagact ataaatgcaa cattgacagc aggtgttaca
     6661 tattatctga ttgttgatac ttggccttct ccaacttgta ttccgagttt caatcttaat
     6721 atatcgggcg gagcacccgg tccatgcgac aatatagtat cgctgccttg cggtacatct
     6781 gccactgcta ccatgtcagg aacaggatcg ttagccagtt caagtgcatg tggttggtca
     6841 acacccggtc aggagaaaat atattccttc acacctactg cttccggaaa tgctacgctt
     6901 gtagtatcat caaccaatag cgcagggtat gttgactatc aatacaaggc agcatcaggt
     6961 ggttgtaatg gtacaggctg gacctgtatt attgatttgt tttcaccagg ttcagcaacg
     7021 tttccggtta cggcgggagt tacatactat ctgttgcttg atccagaaac aacctcatct
     7081 gtgacccata cattccaggt gaattgtcct tcgttaggta gtgtcattac gatgaattgc
     7141 ccggcagctg tatcggtatc gtgcgcgtca caagtgccac cgccaaaccc tggttcggtg
     7201 attgcatcaa cgacttgttc gccgactaca gttactgtca ttcacctaag tgatgtgata
     7261 agtgcccaga cttgtgccaa cagatatacc attacacgaa catatcgtgc ctctgatgcc
     7321 tgcggtacta ccaaaacttg tacacagact atcactgtga atgaccagac accgcctgtg
     7381 gtcaattgcc ctgcgaatgt aaatgtaaaa tgtgctgcgc aggtacctgt ggttaatcta
     7441 aatcaggtga ccggagcaga caactgtaca gggtcggtaa cgttttcgta tcttggtgag
     7501 gtaataagtg atcagctatg tgacaaccaa tataaaataa ccagaacatt ccgtggtaca
     7561 gatgcatgtg gcaattcagc cacgtgtgca caggttattg aagtgaaaga tgatgtaaaa
     7621 ccggttttag gtaccattcc ggcagatgtt accttatctt gtacagaccc gatacctgca
     7681 ccacctgtag ttaccggaac ggataattgt gccggatctg tgtctgtgac attcaaagaa
     7741 actagtacac aaagcgatta tcctacactt tgtagagcgt attcttatga aataacaaga
     7801 gtgtggacag ccaaggatgc ctgtaaaaat acatcattta aggtacagaa aattacggta
     7861 caggatacca aagcacctga atttacatac actccaccac agttcattac tgtagagtgt
     7921 gatgatgatg gtagtaataa tgaagatccg attgcagtag atgattgtga tggtaatcca
     7981 tcagtaatac tgaatgtgaa atataaattc ggacttgaag gatgtgcagg caactatata
     8041 gcagcatata catggacagc cggcgacaag tgtggcaata ccagccaata tacccaatac
     8101 ataacagtta ttgactcaga agcaccacag attaagtgtc cgaacaatgt ggaagtgagc
     8161 agcccgattc caatagccgt tacatggcag tcgccaaaag gtagtgacta ctgcgacggt
     8221 ccgattattc cggtacagat tcagggtcct cctccgggta gtatattccc tgcaccaagt
     8281 aagacgataa tcgtgtatca ggcaactgat aattgtggca atgtatcaac atgttcgttc
     8341 cttgtaatcg ttcgtacagg taatgaaggt tatgttacag gtataggaag tgagacatca
     8401 ttgaacaatg ccgtacaatc aagccaatct gatgctgcta cctaccttgg tatgaccacg
     8461 ctgtatcaaa atgaaccgaa tccgtttggt aacaatacga ctatcagatt cgatcttgaa
     8521 acagcaggta cagcagttat ccaaataatg gatattaccg gaaaaacgat aaggcaaata
     8581 caaggcgaat atccttcagg caaaaacttt atcaatatcg acaacagtca gcttcctgca
     8641 agtggtatgt actactatca gttgaggaca aaagacttta acgatacgaa gaagatgttg
     8701 tatattaagt aaaaatatta attcttttcg aatgccctcc ggtatttcta tcggggggca
     8761 tttcttgtga taatatgtgt agatgtagca ataaaaaaga gagccgattg ttaaatattt
     8821 tttactatac agttacaata ttttagatac aaataatttt cctaactatt tacaccattt
     8881 ataattatgc taattaatct acattttaaa aaaattagaa atgtaagaat tattggaata
     8941 tttttattat gtattcttat aagttttaat ctcttctctc aatgcaacaa tggctcatct
     9001 tatggatctg ctattgcgcc aactaattct gcacctgtga ccattactac atgtgccttc
     9061 gcaggtgaat atagtacaat caccagtgcg gtagccggtt caacatacca atttgtggcg
     9121 acaggtggtg caggtaatta tattaccatc cgtcaaggtt ccccttccgg tacggttata
     9181 ggttacggga attcaccatt aaacgtaaca tgtacagtat caggctcatt atatatgcac
     9241 ctaaatacta atagctcttg tggtaccgat gcatcatgtc acacggaaac cattcaatgt
     9301 acaagctgtg gatcgggtgg atcgggctgt ccttcaggtt tgggttctta tgttaatatt
     9361 acattgccat ataatgggac aggattgacc aattgtggta aaggtaatga tattaccagt
     9421 ataaatgtaa gccctatatg tgggtcatca tcttattatg gtggtgaaga tgaagtttat
     9481 atatttacac ctgcttctac tgcttcttat acggttagct ttacgagcag cagtagttgg
     9541 gttggtctta tgttatatgc cggctgccct ttgacaggtg gagggg
//
LOCUS       DAOQQY010000079         9579 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2662_length_9579_cov_4.895212, whole genome shotgun sequence.
ACCESSION   DAOQQY010000079 DAOQQY010000000
VERSION     DAOQQY010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2662_length_9579_cov_4.895212"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            562..738
                     /locus_tag="PLE23_05475"
     CDS             562..738
                     /locus_tag="PLE23_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82750.1"
                     /translation="MNNTRKILLLCLLATMLTAGISCSISIGHYQHYTHHQGSTIQLH
                     LTHTDTTTDTDAKH"
     gene            725..1237
                     /locus_tag="PLE23_05480"
     CDS             725..1237
                     /locus_tag="PLE23_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX82751.1"
                     /translation="MQNIDQSDRFVSIIEANKAIIYKVANSYCHNTEDRKDLVQEIII
                     QLWKSFATYDDQYKYSTWMYRIALNVAISFYRKEVSRKKISDPLTDSIIRFTDTDDTD
                     DSLPELKLLRHLISQLPDLDKALMLLYLEEKSHKEIAQIIGISETNVATKINRIKNSF
                     KQKFNTYKTT"
     gene            1239..1898
                     /locus_tag="PLE23_05485"
     CDS             1239..1898
                     /locus_tag="PLE23_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82752.1"
                     /translation="MENFELIKIWKEQNAKIEKSLAINELLLKELINTKARNTLRSLI
                     SLKTAGIIAFILYLMLLAYALVYAVSHYSSAWLYFIISVTAIAIINIKGLADYIKHLT
                     WARRIDYNGTIIDIQRQLSRLQLSIISHAKIMCLQFPFFTTFYLSDSWFPQHVGTGYI
                     IFQILLTGSFIVLSYWLYKNHKPENLSKPWFRNLIAGSGGKSVMKAIEFYRELEAFKN
                     S"
     gene            complement(1961..2539)
                     /locus_tag="PLE23_05490"
     CDS             complement(1961..2539)
                     /locus_tag="PLE23_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82753.1"
                     /translation="MHYPQVRVSFGKELTVEEKSDIAQKLNNSKIAEFSIDGDYFIVS
                     LIDFDSETRQQKLEKYEKKWNTITALFRGDGGKDRGNKTTVISENFRKSKYVGARNEG
                     DEQSQTREYDRSDLFETHASQKAGTRTIDPDLNTPMVQAMAVFRESPQYANHKEEAKA
                     YRETLKSIDTLMVRENSRHEVRRIAQPDREAP"
     gene            complement(2889..4430)
                     /locus_tag="PLE23_05495"
     CDS             complement(2889..4430)
                     /locus_tag="PLE23_05495"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HMX82754.1"
                     /translation="MKNKLDILHSKLNESKLGGGLDRIKKQHEKGKLTAHERIQYLLD
                     DHSFEEIDALVTHRNTDFGMDRQVILGDGVVTGYGNIHGRLVFVFSQDFTVFGGSLSE
                     THAEKICKIMDLAVKNGAPVIGLNDSGGARIQEGVNSLGGYADIFYRNTLASGVVPQI
                     SAIMGPCAGGAVYSPAITDFIFMVEHTSYMFVTGPNVVKTVTNETVTSEELGGASAHS
                     TKSGVTHFTASNDVDCLLKIRRLLKYLPQNCEDKVPRIRYELSDELRPALNQMIPAEP
                     NQPYDMRDIIRVIVDDDSFMEVHENYAENIVIGFAHLAGRSIGIVANQPMSLAGVLDV
                     ESSKKAARFVRFCDSFNIPLLVLVDVPGFLPGTDQEWHGIITNGAKLLYAFCEATVPR
                     VTLITRKAYGGAYDVMNSKHIGADMNYAWPTAEIAVMGAKGATEIIFRKEIDEAADPD
                     GKLKEKETEYIEKFANPYLAAQRGFIDEVIEPSVTRRKLIKAFSMLEHKTVSNPKKKH
                     GNIPL"
     gene            complement(4440..6662)
                     /locus_tag="PLE23_05500"
     CDS             complement(4440..6662)
                     /locus_tag="PLE23_05500"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX82755.1"
                     /translation="MSQQKKPTHVKPKALEDIKTSRTSLFSGFFRNINTTTTHDSLLK
                     KIFTGLSALFFVITLILSQKAGINADDRMQNEYEQNLMKFYATMGRDTGALNMPKTQM
                     HFYGGVFEVLSGVTNKILGQDNPDLPGYHKVRHAWSAVFGTSAMVFTGLMAAALGGWL
                     AAIIALVLIFFTPSFLGHSLMNPKDIPFALGYIMSIYYTYKLLIQMPAPKRSTLLLLG
                     VGLGISLGVRAGGLMNFAILAMMLGLHFLATHGFAAITRDTKTLLAYLKAFFIPALSG
                     WVIALLFWPYAMHGPVSNILSSLSTLADYGVNIRLLFNGEMYFAQSLPKSYLPMWLLV
                     TVPVIVLLGWLLHLIFIGSIWKKYNPGGLFLILFAFIFPFAYVMYKGSTIYDGWRHML
                     FPYTAGIVVAALAFAFLADKFSQKPAWHYGVLGIVALGCLSPAIFTIKNPAYPYVYFN
                     ELVGGIQGADGKWENDYWGTSVKQGVEYLEQQGILHEGMKDTVRIASNFFYPLDKYVK
                     HKYGGHVKVIYLKYRQRYDQPWDYALFVNRFMDASYLQKKNFPPASSIHAITANGVPL
                     LAILRDTSHHTTAGMAELRKQNWQQAIKHFDMAIKEDPKNEVAYAGMANAFLTGQQLK
                     EVKIYVDKCLEIDPDNSGALNYLGMYYINNKEIDKGAETFQRAIDIAPNNSIGYYYLA
                     LIAHEKGNLQEAAEKAARSIETNPKFAAGYTLLASLYNEAGMKDKAQEIIDMMPKDAK
                     "
     gene            6983..8194
                     /locus_tag="PLE23_05505"
     CDS             6983..8194
                     /locus_tag="PLE23_05505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX82756.1"
                     /translation="MKKLLSVYKNSFSGLDRIIWLLSAVMLVNRVGSMVLLFLSLYLT
                     KELHFSLSQVGVVMGVYGAGSIVGSYIGGWLTDRYDYYKIMVWSLIISGFILPLLVFV
                     KDYYGIMAIVFSYSVVADAFRPANSVAIGYYSDETNKTRSFSLMRMAINLGFTIGPAI
                     GGFVAAYASYDLLFILDGVTSMLAAVFILLFIPHNKVNPKKQEEEGEKEVGLSAYKDH
                     VYLMFIVLVSCYAIAFFQYFTTIPLYFSKSLKLSEDMIGLIMAYNGILIVLLEMPMVH
                     QLEKRGRLMLNILIGCVLMVLSYLVILPDTGPLTGPILYMTLVTFSEIFAMPFMINFA
                     LTRASHGRQGQYMGLYTIAYGIAHIIAPMLGMRLADSLGFFQTFAITAGFSFLVVLGF
                     YLLRRKNPQIA"
     gene            8252..>9579
                     /locus_tag="PLE23_05510"
     CDS             8252..>9579
                     /locus_tag="PLE23_05510"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HMX82757.1"
                     /translation="MEKFSLCMLFLLITMMFARIAYSQEQAYYPDPDPLIQKRIEEWK
                     DLKFGLLMHWGPYSQWGIVESWSICPEDVSWATGARIAKDSVSDYFTYLKNYENLKSS
                     FNPVKFDPQRWAKAASDAGMKYVVFTTKHHDGFCMFDTKTTDYKVTDPACPFSANAKA
                     DVTKEIFNAFRNENMWVGAYFSKPDWHSQYYWWNRFPPLDRNVNYPIKHYPDRWKKFV
                     DYTYSQIDELVSNYGKLDILWLDGGWVRKKTEDEEKQAMADAFRVYGFGVNAISQDIN
                     MPRLAKLARAKQPQLIIVDRDVPGEQQNYLTPEQHIPDLGLPYPWETCMTMGNSWSYV
                     PNDVYKPTAEIIKKLVDIVSKGGNYLLNIGPSPEGEFDTVAYQRLEEIGKWMKMNSEA
                     IYSTGMYKVFGEGENIRFTQSKDGKTIYVFLLEIPDEKLLLQKLNPSKNA"
BASE COUNT         2553 a         2101 c         2168 g         2757 t
ORIGIN      
        1 tacaggacgc gcaggttcag caggtaaggc attttcatta tgctcgactg acgaactcga
       61 ctacatgaaa gatatcaata aactcatcaa acaaaaaata gaggtcgttg actcacaccc
      121 tttcgtttga tgtttaaaaa tgtattggat acccaacggc aacacccagt gctggaaata
      181 caaccacgca gcagtgttca aatcataata cagtgtaatc ctatgttggt tctttccggc
      241 tgacaatatc cgtgttgttc attagggcgt gcccctgtcg ggtcgggcta tccgccttac
      301 gttgtgccgc ctaccatcct tcacgcaact cgggtgatta gttagtacac aaacttttgt
      361 cctctttatc gcagccaagt atatcactta tgattacgat gggtaagaca aaatcttgcc
      421 agcaaagtat gtatccagat tgacttatgc accacactca tcactcacag gaaaaaaatc
      481 cttcccatct ttgtgatatt tcgccacctg atgcgaataa tagagaaatt catacattta
      541 acttattatt tttcgaagca aatgaacaat acaagaaaaa ttctactgct ctgcctgcta
      601 gccaccatgc ttacagccgg cattagctgc agtataagca tcgggcatta ccagcattac
      661 acacaccacc agggcagcac catacagcta catcttacac ataccgacac aaccaccgac
      721 accgatgcaa aacattgacc agtctgaccg atttgtttca atcatcgaag ctaacaaggc
      781 catcatctac aaggttgcca attcgtactg tcacaatacc gaagaccgca aagacctggt
      841 acaagaaatc atcatccagc tttggaaatc atttgccacc tatgacgacc aatacaaata
      901 ttccacctgg atgtaccgca tcgcacttaa tgtcgccatt tctttctacc gcaaagaagt
      961 gagcagaaag aaaatctccg atccgctcac agacagcatc attcgtttta ctgatacaga
     1021 cgacaccgac gacagccttc ccgagctcaa gctactaagg catctcattt cgcaactacc
     1081 tgatctcgac aaagccctga tgttacttta cctcgaagaa aaaagccaca aagagattgc
     1141 tcaaataatc ggtatttccg aaaccaatgt ggctacaaaa atcaaccgta tcaaaaatag
     1201 tttcaaacaa aaatttaaca catacaaaac cacataatat ggaaaatttt gaactcatca
     1261 aaatctggaa ggaacaaaat gccaaaatcg aaaaatcatt ggccatcaat gagttactcc
     1321 tcaaagaatt aatcaacacc aaagcacgca acacgctacg gtcactcata tcgctcaaaa
     1381 ctgccggcat catagcattc attctttacc tcatgctgct ggcctacgct ctggtttatg
     1441 ccgtatcaca ttactcatcc gcatggcttt acttcatcat ttcagtcaca gccattgcca
     1501 ttatcaacat caaaggcctt gccgactaca tcaagcacct cacctgggcc agacgtatcg
     1561 attacaacgg caccatcatt gacatccagc gacaattgtc acgcctacag ttatccatta
     1621 tcagccatgc aaaaataatg tgcctgcagt ttccgttttt taccaccttt tatctcagcg
     1681 attcctggtt tcctcagcat gtcggcaccg gatatattat tttccagata ttactcaccg
     1741 ggtcattcat cgtccttagc tactggttgt acaaaaacca caaacccgaa aatctcagca
     1801 aaccatggtt ccgaaatctc atagcaggct caggcggcaa gtcagtgatg aaagccatcg
     1861 aattctatcg tgagcttgag gctttcaaga atagctgatt gagcactttt tattccggtc
     1921 accggcacga taacaagaaa cctgacaact acatttttca ttagggcgcc tccctgtcgg
     1981 gttgggctat acgccttact tcgtgccggc tattttccct caccattaat gtgtctatgg
     2041 attttaaagt ttcacgataa gccttagcct cttccttatg gttggcatac tggggtgatt
     2101 cacgaaaaac agccatcgcc tgaaccatag gcgtgttgag gtcggggtct attgttcggg
     2161 ttcctgcttt ttgggaggcg tgtgtttcaa aaaggtcact tctatcgtat tctctcgttt
     2221 gcgattgttc atctccctca tttcttgcac cgacatattt ggatttccta aaattttccg
     2281 aaatgaccgt agttttattt cctctatcct tgcctccatc tcctctgaat aaggctgtaa
     2341 tggtgttcca tttcttttca tatttttcta atttttgttg tcttgtttct gaatcaaagt
     2401 ctatcaaaga tactataaag taatctccgt caatagaaaa ctctgctatt ttagaattat
     2461 ttaacttttg ggctatatct gacttctcct ctactgtcag ttctttaccg aaacttaccc
     2521 tgacttgcgg atagtgcata tacccatctt catcaaattc cgtaagcggt acccaatctg
     2581 taatctcgga ttccgtcttg aagttcctcg aaccgttttt tgttgcttag gtttcctttc
     2641 gctattttcg atgctactag actttctcaa taaagtatag acaaatttga ccccgtaaca
     2701 caaatctcgc ctaagagact gagtggtatt ccattatcgc atgttctacc ttcttgcgtc
     2761 aaggatagaa gccggcacga agtaaggcgt atagcctgac ccgatagggg gacgcccaaa
     2821 aaactgaaag ctttgttatg gataatccat gctcagcctt tatcttactc cgtgtattat
     2881 ttgctccact acagcgggat gttgccgtgt tttttcttcg gattggaaac ggtcttgtgt
     2941 tcgagcatgg agaatgcctt gatgagtttg cggcgtgtaa ccgaaggctc gatgacctca
     3001 tctatgaaac ctcgctgggc tgcaagataa ggattggcga acttctcgat gtattcggtt
     3061 tccttctctt taagtttacc atcggggtcg gctgcttcgt cgatctcctt gcggaatatg
     3121 atttctgtag cacctttagc gcccataacg gcaatctcgg cagttggcca ggcgtaattc
     3181 atatcggctc cgatgtgttt ggagttcata acatcgtaag ctccaccgta tgctttgcgg
     3241 gtgatgagtg tgacacgtgg tacggtggct tcgcagaatg catacaagag cttggctccg
     3301 ttggtgatga taccgtgcca ttcctggtcg gtgccgggca agaaacccgg tacgtcaacg
     3361 agtacgagca gtggtatgtt gaagctgtcg cagaagcgta cgaaacgggc tgcctttttg
     3421 gatgattcta catcgagcac gccggcgagt gacatcggct ggttggctac gatgcctatg
     3481 ctacgaccgg cgaggtgtgc aaaaccgatg acaatgtttt cggcgtagtt ttcatgtact
     3541 tccatgaagg agtcatcgtc aacgataacg cggataatat cacgcatgtc gtaaggctgg
     3601 ttgggttcgg ccggtatcat ttggttgagt gccggacgga gttcgtcact gagttcgtac
     3661 cggatgcgtg gcaccttgtc ttcgcagttc tgtggcaggt atttgagcag acgtcgtatc
     3721 ttgagcaggc aatcgacatc gttagacgct gtgaagtggg tgacacccga cttggtgctg
     3781 tgggcagaag cgccgccaag ttcttcggac gtaaccgtct cattggtgac tgtcttgacc
     3841 acattggggc ctgtgacgaa catgtaagac gtatgctcta ccataaagat aaagtctgta
     3901 atagccggag aatatactgc gccgcctgca caggggccca tgatggccga tatttgtggc
     3961 acaacacccg aagcgagtgt attgcgatag aaaatatcgg catagcctcc aagagagttt
     4021 acgccttcct gtatgcgggc accgcctgag tcgttgagac cgatgaccgg agcgccgttt
     4081 ttaacagcca ggtccatgat tttgcatatc ttctcggcat gggtttcaga cagagaacca
     4141 ccaaataccg tgaagtcctg cgagaagaca aataccagcc gaccatggat gttaccgtag
     4201 cctgtgacta cgccatctcc gaggattacc tgcctgtcca taccgaagtc ggtgttgcgg
     4261 tgcgtgacga gtgcgtctat ctcttcgaat gaatggtcat cgaggaggta ttgaatgcgt
     4321 tcgtgagcgg tgagtttgcc tttttcgtgt tgttttttta tccggtcaag gccgccaccc
     4381 agcttcgatt catttaattt tgagtgcagt atgtcgagtt tatttttcat gcattgttgt
     4441 tatttagcat cttttggcat catgtcaata atttcctggg ctttgtcttt catgcctgct
     4501 tcgttgtaga gagaagcaag cagcgtatag ccagcggcga attttggatt ggtttcgata
     4561 gagcgggctg ctttttcggc tgcttcctgc agattgcctt tttcgtgggc tatgagggct
     4621 aggtaataat agccaatgct gttgttgggt gctatatcta tggctctttg gaaagtttca
     4681 gcacctttgt ctatctcttt gttgttgata taatacatgc cgagatagtt gagtgcgccg
     4741 ctgttgtcgg ggtctatttc gagacattta tcgacatata tttttacttc tttgagctgc
     4801 tgaccagtga ggaaggcatt ggccataccg gcgtaagcta cttcgttttt cggatcttcc
     4861 tttatagcca tgtcgaaatg tttgatagcc tgctgccagt tttgtttgcg aagctcggcc
     4921 atgcctgctg tggtatggtg gcttgtatct cgtagaatgg caagtagtgg cacgccatta
     4981 gctgtgatgg catgtataga cgaagccggt gggaaattct ttttttgtag gtagctggca
     5041 tccatgaagc ggttaacgaa gagtgcatag tcccatggct ggtcgtaacg ctggcgatat
     5101 ttgaggtaga tgacctttac gtggccgccg tatttgtgtt tgacatattt gtccaatggg
     5161 tagaagaaat tggatgcaat gcggacagta tctttcatgc cttcgtgcag gatgccttgc
     5221 tgttcgaggt attcgacacc ttgttttact gaagtgcccc aatagtcgtt ctcccatttg
     5281 ccgtcagcgc cttggatgcc acctaccagc tcattgaagt aaacataagg gtaagcagga
     5341 ttttttatgg taaaaatagc ggggctcaga caacccaatg ccactatacc taagacgccg
     5401 tagtgccacg ctggtttttg cgaaaatttg tcggcaagga aggcaaatgc cagagctgcg
     5461 accacgatgc cagccgtata agggaacaac atgtgccgcc agccgtcata gatagtagag
     5521 cctttgtaca tgacataggc aaatggaaat ataaaggcaa acaatatcaa aaacaagcca
     5581 cccggattgt atttcttcca gatactccct atgaaaataa gatgcagcag ccagccgagc
     5641 aatacaataa caggtactgt taccagaagc cacataggta ggtaactctt aggtagcgat
     5701 tgggcaaagt acatttcgcc attaaacaat agccggatat taacaccata atcggccaga
     5761 gtgctaagcg aactgagtat attgctgaca gggccgtgca tggcgtatgg ccagaagagc
     5821 agggcaatga cccagcctga gagggcaggt atgaagaaag ccttcaggta ggcaagtagt
     5881 gttttcgtgt cgcgtgtaat ggctgcaaat ccatgtgtgg caaggaagtg gagaccgagc
     5941 atcatggcca ggatggcaaa attcataaga ccacccgctc ttacccctaa tgaaatgcca
     6001 agacccacac ccagcaacag gagtgtactg cgtttcggag ccggcatctg tatgagtaat
     6061 ttgtatgtgt aatatatgct catgatataa cccaaggcga aaggtatatc cttagggttc
     6121 ataaggctgt gccccagaaa actcggagtg aaaaagatga gtacaagagc aataattgct
     6181 gccaaccagc caccaagggc tgccgccatt aatccggtaa agaccatagc tgatgtgcca
     6241 aagactgcag accaagcatg gcgcaccttg tggtagcccg gcaagtcagg attgtcttgt
     6301 ccaagaattt tgttcgtgac acccgataac acttcgaaca caccaccgta gaagtgcatc
     6361 tgtgtctttg gcatattgag cgctccggta tcacgaccca ttgttgcgta aaacttcatg
     6421 agattttgct catattcgtt ctgcatgcgg tcgtctgcat tgatgcccgc cttctgtgat
     6481 aagatgagtg taatgacaaa aaagagggca gacaggcctg tgaatatttt ttttagaagg
     6541 gaatcatgtg ttgttgtcgt attaatattc cggaaaaagc ctgaaaacaa gcttgttcga
     6601 gatgttttta tgtcttccaa ggcttttggc ttgacgtgtg ttggtttttt ttgctgactc
     6661 actttgatta tatattagct tacagatgtg caaatataac ggatgcatca ataaatttgt
     6721 gaccgacagg ccatttatta taatgtgtgt gtaatttaag tgaatattta ttcaataaaa
     6781 atgtcagtaa ataacgcgtc gattcatgta atgattatcc aacaataaat gcgatggagt
     6841 gctctgtgtt tactcaatac catcttcttg tttgttgcaa cataacagaa tgattttttt
     6901 tgaaattatc ttgtgaaaat tttcatttaa aaaaatatta tgtattctta atcaatatag
     6961 ggaaaattat gttaatgtca ggatgaagaa gctgctgagt gtgtataaaa attcgttttc
     7021 gggcctggac cgtatcatct ggctattgtc ggcagtgatg ttggtaaaca gagtagggtc
     7081 gatggttctg ttgtttcttt cgctttacct taccaaggaa cttcactttt cattgtcgca
     7141 ggtgggtgtg gtaatgggtg tatatggtgc aggcagcatt gtgggcagct atatcggcgg
     7201 atggctcacc gaccgatacg attattacaa aataatggtt tggtcactca ttatcagtgg
     7261 gtttatacta ccgcttttgg tgttcgtcaa ggactattat ggtatcatgg ctatagtgtt
     7321 ctcctatagc gttgtggcag atgcctttcg cccggccaat tcggttgcca taggttatta
     7381 ttctgatgaa accaataaga cccggtcatt ctcactgatg cggatggcta ttaatctcgg
     7441 gtttacgatt ggtccggcta taggtggttt tgtggcagct tatgccagct acgatttact
     7501 gtttattctc gacggagtta catccatgct tgccgcagtt tttatccttt tatttattcc
     7561 acacaacaag gtgaatccga agaagcaaga agaagagggt gaaaaggaag tcggtctttc
     7621 ggcttacaaa gaccacgtgt accttatgtt tatagtactt gtgagctgtt atgccattgc
     7681 attttttcaa tattttacca cgataccgct ttatttctcc aagagtctga aactgtcaga
     7741 agatatgatt ggtctcatca tggcatacaa tgggatattg attgttttgc tagaaatgcc
     7801 gatggttcat cagctcgaga agcgaggtag attaatgctt aatatcctca tcggttgtgt
     7861 gctgatggta ttgagctacc tggtcatttt gcccgatacc ggacctttga cagggcctat
     7921 actttatatg acgcttgtta ccttttctga aatatttgcc atgccgttta tgattaattt
     7981 cgcgttgacc agggcaagcc atggtaggca gggtcagtat atgggcttat atactatagc
     8041 atacggcatt gcacacatca tagcacccat gttgggtatg cgcttggctg actcgcttgg
     8101 tttttttcag acattcgcca tcacagcggg gtttagcttt cttgtagtcc ttggttttta
     8161 tctgctgagg aggaagaatc cgcagattgc ataagttgta cttagaatcc attaatattt
     8221 cgtaatatta tggctgaaaa tatctttatg tatggaaaaa ttcagcttgt gtatgctatt
     8281 cctgcttata actatgatgt ttgcgagaat cgcttattct caggaacagg catattatcc
     8341 cgaccccgac cctttgatac aaaaacgtat tgaagaatgg aaagatttga aattcggatt
     8401 gctgatgcat tgggggccat acagccagtg gggcatcgtt gagagctgga gtatttgccc
     8461 ggaagatgtt tcctgggcga caggtgcacg aattgcaaag gattcggtga gcgattattt
     8521 tacttattta aaaaactacg aaaatctgaa atcgagcttt aatccggtga aatttgatcc
     8581 tcaacgttgg gcaaaagccg catctgatgc cgggatgaaa tatgtcgtat tcaccaccaa
     8641 acaccatgac ggattctgca tgtttgatac taaaaccacg gattataaag taacggatcc
     8701 tgcttgtcct ttttcggcca atgcaaaggc agatgtgacg aaagaaattt ttaatgcatt
     8761 ccggaatgaa aatatgtggg taggcgccta tttcagcaaa ccagactggc attcgcagta
     8821 ttattggtgg aaccgctttc cgccattaga cagaaatgtc aactatccga tcaaacacta
     8881 tcctgaccgg tggaaaaaat ttgtagatta tacctattcg cagatagacg aactcgtcag
     8941 caattacggg aaactcgata tcctttggct agatggtggc tgggttcgga aaaagacaga
     9001 ggatgaagaa aaacaggcaa tggcagatgc attccgggtg tatggttttg gtgtaaacgc
     9061 catcagtcag gatataaata tgcctcgtct ggcgaaactg gcaagagcca aacaacctca
     9121 gttaatcatt gttgacagag atgttcccgg tgagcagcaa aattatctga ctcccgagca
     9181 acatattcct gacctgggct tgccttatcc ttgggagacc tgcatgacca tgggcaacag
     9241 ctggagttat gtacccaatg atgtctataa gccaacggca gaaatcataa aaaaactggt
     9301 tgatattgtg agcaagggcg gtaattacct gctcaatata ggcccgtctc ctgaaggaga
     9361 attcgatacc gttgcctatc agcgcttaga agagataggt aaatggatga agatgaacag
     9421 tgaagccatt tattccaccg gcatgtacaa ggttttcggt gaaggagaaa acatccgttt
     9481 cacccaaagc aaagacggca agacgattta tgtgtttttg cttgaaattc cggacgaaaa
     9541 actgctgcta cagaagctga atccatcgaa aaatgcaaa
//
LOCUS       DAOQQY010000080         9489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2691_length_9489_cov_5.377040, whole genome shotgun sequence.
ACCESSION   DAOQQY010000080 DAOQQY010000000
VERSION     DAOQQY010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2691_length_9489_cov_5.377040"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1183
                     /locus_tag="PLE23_05515"
     CDS             <1..>1183
                     /locus_tag="PLE23_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82758.1"
                     /translation="IIRTWTATDDCGNSAQATQKISVVDVVPPVFTYVPVSGTIECSV
                     PLTGLSTDAKASDNCDNKVDISYHDVTIPGKCPSEYTLIRTFTAKDDCGNSSTAVQQI
                     NVVDTTKPLVEFKNPIFAGMSAGDTLSLECGTYKGFGPADFTFFDNCDQHPEFKLVEN
                     IIVNQACYKLLKCIVTVTDACGNVGSLEFFVEIKDTMSPILSNCPPSVTIECDQPLPA
                     IPNVKATDGCNQQPVVHFTENTLPGNCPQEKTILRTWTADDLCGNTAQCSQTISVQDT
                     KAPQIDFKNPIFTGLSEGDTVKVECGVFNGFGPDDFTITDNCDPNPVFDMVDIFIVNT
                     PCYKLLKCIATVTDACGNATEFIFYVEVTDTQAPVLANCPANITIECDQPVPSAANVT
                     AT"
     assembly_gap    1184..1283
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1284..7093
                     /locus_tag="PLE23_05520"
     CDS             <1284..7093
                     /locus_tag="PLE23_05520"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82759.1"
                     /translation="ACDPHPKVNFTENTLPGKCPQEKTIVRTWTAIDACGNTAQCSQS
                     ISVQDTKAPVIHFNPANPLFTNFSEGDTVKVECGVFGGFTPDDFLVTDNCDPHPVFDM
                     VDIIIINTPCHKFLKCVASATDACGNVGQLIYYVDITDTQAPVLANCPANITIECDQP
                     VPSAANVTATDACDPHPKVNFTENTLPGNCPQEKTIVRTWTAVDACGNTAQCSQSISV
                     QDTKAPVISINIPGFTNGDTITVDCGAPIPAFNKTNVVVTDNCSTDIDVEFCECTYQG
                     DCETTGFYELITYYVKATDECGNSSIFEFSVKVIDNTPPLISGVPDDVTVSCAAGLPS
                     LPTVTADDECSETVQLTFAENTAPGKCSGEKIITRTWTAVDECGNVATDSQVIQIIDD
                     TPPTLVNVPSDLTISCDVTIVPAGNVSATDDCSTNATVSVDEKHVPGSCANSYTIVRV
                     FTATDDCGNTAQATWKIFVVDEKAPVFTNVPASITVECSDPVPAPDQSAAAKDNCDPD
                     VDISHIDAAVPGNCPQEYTIIRTFIATDNCGNTVTAVQEIKVKDTTAPGIQFDPSNPL
                     FAGFSEGDTVKVACGTFNGFGPDDFILTDNCDPNPVFDMVDIFIVNTPCYKLLKCIAS
                     ATDACGNVSQLVFYVEITDTQAPVLANCPVDITIECDQPVPPAANVTATDACDPAPKV
                     NFTENSIPGNCPQEKTIIRTWTAVDECGNTSSCSHKIEVQDTKAPEYTLNGDLTGVPN
                     GDTLIIDCNGVLTMDMKSVMFTDNCDPDVPSIFTETLQSGDCQKDGYITFLECTWTGT
                     DDCGNSTSYTIYIKVVDNTPPVITNCPADITVDCGVQIPPAGQLTATDDCDQDVKISF
                     TENVHAGKCAGDQIVERTYTATDDCGNTATCKQIITRTDNEAPVIAGVGQDVTIECGQ
                     ALPDPGKVYATDNCDPNPVLKYSVQHTPGQCAGSYVEIRTWQATDACGNTAVKTQKVT
                     VQDTKAPHFAGLPASLTVECSDPITDLGKDVKAVDDCDQDVAVTFKDITVPGKCAHEF
                     TIIRTFAATDDCGNTVTAIQEIKVKDTTPPVIDFDPSNPLFAGFNEGDTVKVECGVFK
                     GFGPDDFILTDNCDPHPVFDMVDIFIVNTPCYKLLKCIASATDACGNVSELVFYVEMT
                     DNLPPVFDNCPSDLVIECDQPVPPSGKVTATDACDPNAEVHFTQSEVPGKCANEKTII
                     RTWTAKDHCGNTAVCVQKIEVKDTKAPEYILTGPLVGVAFGDTLVLGCDEQLTMDMKS
                     VNFVDNCDPDVPSIFTETLLAGDCQKDGFVTLLECTWTGTDDCGNATAYTIYIKVVDK
                     KVPILSGVPANITVDLSKGEQIPGAPKVTASDNCDLDVDVTYQEVVDTAHCGGHILRT
                     WTATDDCGNVAQATQVITVVKDCPCIDPVIANIKVKDADCGDDNGRVEIVISNGANNF
                     EYEWIPDAGVSIGAGNIREGLPAGNYTVLVKYPSSYPCITTLNITVKGTGGNIIAEDT
                     LIVANAKCNKPVPVCLGLNVLEVPNYDIYDNGVIYTGNIAGCDFDTVMSYTYATIPGM
                     GQNGPYVLDSWVVNGLVLTGEFDNLNALVALMNVLDPQGNWALNTTTLSITGGSKAKV
                     YGQMKISKKVGSSKATLDINIQLIPNGTSLNLSTGAHEIVLVNKATHCSDAVYVKVFC
                     LTPNIVNVKVFVTETKQVCVDNSQLQGTPVSIANVCDNLSGEFSLVNITSTGYCVNIT
                     GIEPGIEKACIVVCDDLGFCDTTYINITVYETNLKPIAVDDYLTASAGKSKTVQVLAN
                     DIFADGLLSMKIISSPTTGKVTSFIDKVVVVTPDKEDCQPMVFDYEICNQYGCDTGTV
                     HVNVLCKGLIIYNGMTPNNDGLNDYFTIDGIEDYPNNELSVYNRWGNMVYSAKAYNNN
                     WDGSWNGKLLPDGTYYYILKDGEGESYNGYLQISR"
     gene            7237..7309
                     /locus_tag="PLE23_05525"
     tRNA            7237..7309
                     /locus_tag="PLE23_05525"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7270..7272,aa:Lys,seq:ttt)
     gene            complement(7557..>9489)
                     /gene="feoB"
                     /locus_tag="PLE23_05530"
     CDS             complement(7557..>9489)
                     /gene="feoB"
                     /locus_tag="PLE23_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HMX82760.1"
                     /translation="LYPTAYDEKVVFKILANPNDNLFPDLIVYIADATQLERHLLLFT
                     QLKDLGVPIILGLNMQDLAEARKIKIDAKGLEKSFGTPVVWLNGRTGHGLNELKTKIS
                     EMCSLNEHPTNNFQTYVPDAKTNELSEKIVSKTGAKTKFQALLTAHHVEELQFLNEEQ
                     KEYIKEQTSKLGFDSLHSQIDETMQRYRWIEHSLVKHQSLITADGKNKTHTLDRILTH
                     RVWGPLIFISILLLIFQAVFSWAEWPMQMFETGIIALTELVQNHLPAGWFTELLVDGI
                     LPGLGGVLVFIPQIAILFLFTSFLEELGYMARAIYLFDRLLLKFGLNGRSIISLVSGA
                     ACAIPAIMSARTINNWKERLITIMVTPLISCSARLPVFAVLISFIITDNTFFGPIGMR
                     SLILFLLYSLGLLAALVAAIVFKKLLKTNETSFLMIELPEYKVPHWKSILLAVKSKVQ
                     SFISEAGMIIMVISVALWFLASFGPGNEIESAQKQATEYAASQNMTPEETENLKASAK
                     IENSYAGHIGKFIEPAIVPLGFDWKIGIALITSFAAREVFIGTMSTIYSIGSGDEDPE
                     TLQKRMSKEINANTGQKTFTIAASMSLLIFYLFAMQCMSTLAVVRKETGTWKWPLIQL
                     IYMSALAYFGSWITYVLLS"
BASE COUNT         2788 a         1898 c         2107 g         2596 t
ORIGIN      
        1 atcatcagaa cttggacagc cacagacgat tgtggaaatt ctgcccaggc aacacaaaaa
       61 atatctgtag tcgatgttgt acctccggtt ttcacttacg ttccggtgtc aggtactatt
      121 gagtgcagcg tccctcttac cggacttagc accgatgcca aagccagtga taattgtgac
      181 aataaggtag atatcagcta tcatgatgtg actatccccg gcaaatgccc gagtgaatat
      241 acgctgataa gaacatttac agctaaagat gattgtggta attcttcaac cgctgttcag
      301 caaataaatg tggtggatac tacaaaacct ttagtcgaat tcaaaaatcc gatattcgcg
      361 gggatgagtg ccggtgatac attaagctta gaatgtggta cttacaaagg tttcggtccc
      421 gctgatttca cattctttga taactgcgac cagcaccctg agtttaaatt ggtcgaaaat
      481 atcatcgtca accaagcttg ttataagtta ttgaaatgta tagtgacggt tacagacgct
      541 tgtggcaatg taggcagtct cgaattcttt gttgagataa aagataccat gtcaccgata
      601 ctttccaatt gccctccaag tgttactata gaatgtgacc agccgctgcc ggctatacca
      661 aatgtgaaag caaccgatgg ctgtaatcaa caacctgtgg tgcattttac agaaaatacc
      721 cttccgggca attgcccgca ggaaaaaaca atcttaagaa cctggacagc cgatgatttg
      781 tgtggcaata cagcacagtg cagccagact atcagcgtac aagatactaa ggcgcctcag
      841 attgacttca aaaacccgat ttttacaggt ttatctgaag gagacacggt gaaagtagaa
      901 tgcggtgtgt tcaatggttt tggtccggat gatttcacca taactgataa ttgcgacccg
      961 aatccggtgt ttgacatggt cgatattttt attgtcaata caccttgcta taaattattg
     1021 aaatgtatag ctacggttac agacgcgtgt ggcaatgcta ccgaatttat tttctatgtc
     1081 gaagtaacag atacacaagc accggtactt gccaattgtc ctgctaacat aacaatcgaa
     1141 tgcgaccaac cggtaccatc agcagccaac gtaaccgcaa cagnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnatgcatg cgaccctcat ccgaaagtta attttacaga
     1321 aaataccctt ccgggcaaat gtccgcagga aaaaaccatc gtccgtactt ggacagcaat
     1381 agatgcgtgt ggcaatacag cacaatgcag ccaaagcatc agtgtccaag ataccaaagc
     1441 accggtcata cattttaacc ctgccaaccc tttgtttacc aatttctcag aaggtgatac
     1501 agtaaaggta gaatgcggcg tatttggcgg atttacacct gatgattttc tggtgacaga
     1561 caactgcgac ccgcatccgg tatttgacat ggtcgatatt attattatca atacaccatg
     1621 ccataagttt ttgaaatgtg tggcatctgc tacagacgcg tgtggcaatg ttggtcagct
     1681 tatatattat gttgacatta cagacacaca ggcaccggta cttgccaatt gtcctgctaa
     1741 cataacgatc gaatgtgacc agccggtgcc atcagcagcc aacgtaaccg caacagatgc
     1801 atgcgaccct catccgaaag ttaattttac agaaaatacc cttccgggca attgtccgca
     1861 ggaaaaaacc atcgtccgta cctggacagc tgtagatgcg tgtggcaaca cagcccaatg
     1921 cagccaaagt atcagtgtac aagataccaa agcaccggtc attagcatta acattccggg
     1981 ctttacgaat ggtgatacca taacggtaga ttgcggcgct ccaatacctg catttaacaa
     2041 gactaatgta gttgtaacag ataattgctc tacagatata gatgttgaat tttgcgaatg
     2101 tacatatcag ggcgattgtg aaacaacagg attttacgag ctgattactt attatgtaaa
     2161 agctaccgat gaatgtggaa attcgagcat cttcgaattt agcgtgaagg taattgacaa
     2221 tacgcctcca ctcatcagcg gagttccgga tgatgttaca gtaagttgcg ctgccggctt
     2281 accatcattg ccaactgtaa cagccgatga cgaatgttct gaaacggtac agctcacatt
     2341 tgctgagaat acagcacccg gaaaatgctc cggagaaaag atcattacca gaacatggac
     2401 tgctgtcgat gaatgtggta atgtagctac cgactcacaa gtcatacaga tcatcgatga
     2461 tacaccaccg actttagtaa atgttccatc agacctgacc atcagttgtg atgttaccat
     2521 tgtgccggca ggaaatgttt ctgcaacgga tgattgtagt acaaatgcga ccgttagtgt
     2581 tgatgaaaaa catgttccag gttcttgtgc caatagttat acaattgtaa gggtctttac
     2641 cgctacggat gattgtggca atactgcaca agcaacttgg aaaatatttg tagtagatga
     2701 aaaagcaccg gtgtttacca atgtacctgc aagtattaca gtagaatgca gtgacccggt
     2761 accggcccct gatcagagcg cagcggcaaa agacaattgc gacccggatg ttgatatttc
     2821 acacatcgat gctgctgtgc ccggtaattg tcctcaagaa tataccatta taagaacctt
     2881 cattgctacc gataattgcg gaaatacggt gactgcagtg caggaaataa aagtaaaaga
     2941 taccacagca ccaggcattc agtttgatcc ttccaatccg ctttttgcag gtttctcaga
     3001 aggcgataca gtaaaagtag cgtgtggcac attcaacgga ttcggcccgg atgatttcat
     3061 tctgacagat aactgtgatc ctaatccggt atttgatatg gtcgatattt ttattgtaaa
     3121 tacgccatgt tacaagctat tgaagtgtat tgcttcagcc acagatgcat gtggaaatgt
     3181 aagtcagctt gttttctatg ttgaaatcac agatacacag gcaccggtac ttgccaattg
     3241 ccctgttgac atcacgattg aatgtgatca gcctgttcca cctgcagcca atgtaacagc
     3301 tactgatgca tgtgaccctg ccccgaaggt gaatttcaca gaaaattcaa taccgggcaa
     3361 ttgtcctcag gaaaagacaa ttatcagaac ctggactgcc gtggacgaat gtggcaacac
     3421 ctcaagctgc agccataaaa ttgaagtaca agatacaaag gcaccagaat atacccttaa
     3481 tggagatttg accggagtgc ctaatggcga tacgctcatt atagattgta atggtgtatt
     3541 gaccatggac atgaaatcag tcatgtttac agataattgc gacccagatg ttccaagtat
     3601 ttttacagaa acactccagt caggtgattg tcagaaagat gggtacatta ccttccttga
     3661 atgcacatgg accggcacag atgactgtgg taattcgact tcatacacaa tatatataaa
     3721 ggtagttgac aatacgcctc ctgttattac caactgtccg gctgatatta ctgttgattg
     3781 tggtgttcag ataccaccgg caggtcagtt gacagctacg gatgactgtg accaggatgt
     3841 taagattagt tttactgaga atgtacatgc cggcaaatgt gcaggcgacc agatagtaga
     3901 gcgcacttac acagcaacag atgactgtgg aaatacggca acctgcaaac agattatcac
     3961 tcgtacggat aatgaagcgc cggttattgc aggtgtaggc caggacgtaa ccattgaatg
     4021 cggccaagcc ctgccggatc caggcaaagt atatgctaca gacaattgtg acccgaatcc
     4081 agtgttgaaa tattccgtcc aacatacacc gggccaatgt gcaggcagct atgtcgagat
     4141 cagaacatgg caagcaacag atgcatgtgg caacactgcc gtaaagacac aaaaagttac
     4201 agttcaggat actaaagcac cgcattttgc aggtctgccg gcatcactta cagtagaatg
     4261 cagtgacccg attacagatt tgggtaagga tgttaaagca gtcgatgatt gtgaccagga
     4321 tgttgcagtc acattcaaag acatcactgt gcctggcaaa tgcgcacatg aatttaccat
     4381 tatcaggaca tttgcagcta cagatgattg tggcaataca gttacagcta ttcaagaaat
     4441 taaggtaaaa gacacaacac caccggttat tgattttgac ccatccaacc cactttttgc
     4501 cggctttaat gaaggtgata ccgtcaaggt agaatgtggt gtgttcaagg gttttggtcc
     4561 ggatgacttt atcctgaccg acaactgcga tccgcatccg gtgtttgata tggttgatat
     4621 ctttatagtg aatactccat gctacaagtt gttgaaatgc atcgcctcgg ctacagatgc
     4681 ctgtggtaat gtaagtgagc ttgttttcta tgtagaaatg acagacaatc ttccaccggt
     4741 gtttgataat tgcccttctg accttgtgat agaatgcgac cagccggttc cgccatcggg
     4801 caaagtgact gctacagatg cctgtgaccc gaatgcagag gttcatttta cccaaagtga
     4861 agtgcctggc aaatgtgcta acgaaaagac catcatcaga acctggacag ccaaagacca
     4921 ctgtggcaat acagctgtat gtgtacagaa aattgaagta aaggatacca aagcaccgga
     4981 gtatatactt accggtccgc ttgtaggcgt tgcttttggc gatacgcttg tgctgggctg
     5041 tgatgaacag ttgacgatgg atatgaagtc cgttaatttt gttgataatt gtgatccgga
     5101 cgtaccaagt atttttacag aaactttact tgcaggagat tgtcagaaag acggatttgt
     5161 aacattgctt gagtgtacct ggacaggtac agacgattgt ggaaatgcta cagcttatac
     5221 tatttatatt aaagtggtgg ataagaaagt gcctatactc tcaggcgtac ctgcgaacat
     5281 tactgttgac ctaagtaaag gagagcaaat accaggtgca ccgaaagtga ccgcaagtga
     5341 taactgcgat ttggatgtag atgttactta tcaggaagta gttgacacgg cacattgcgg
     5401 tggacacatc ctcaggactt ggacggccac ggatgactgt ggtaatgtag cccaagctac
     5461 ccaagtaatt acggtggtga aagattgccc ttgtattgat cctgttattg caaatattaa
     5521 ggtgaaggat gccgactgtg gtgatgacaa cggacgagta gaaatagtaa tcagtaacgg
     5581 agcgaataat tttgaatatg aatggattcc ggatgcaggt gtaagtatag gtgctggaaa
     5641 cataagagaa ggattaccgg ctggtaatta tacagtattg gtaaaatatc cttcatctta
     5701 tccttgtatc actacactta acattacagt taaaggtaca ggtggtaata ttattgccga
     5761 agacacatta atcgttgcca atgccaagtg caacaaacct gtgccggtgt gcttaggcct
     5821 gaatgtatta gaagtgccaa attatgatat atatgataat ggtgtcattt ataccggaaa
     5881 tattgccggc tgcgattttg acactgtgat gtcatatact tatgctacca taccaggtat
     5941 gggtcagaac ggtccgtatg tgcttgattc gtgggttgta aacggccttg tacttacagg
     6001 cgaatttgat aacctgaatg cgttggtagc cttgatgaat gtacttgacc cacaaggtaa
     6061 ctgggcatta aataccacga cattgagtat tacaggtgga agtaaggcaa aagtctatgg
     6121 ccagatgaag atttcgaaga aagtgggttc cagcaaagct acactcgata tcaacataca
     6181 attaatacct aatggtacga gccttaacct gagcaccggt gcacatgaga tagtgcttgt
     6241 caacaaagca acccactgtt cggatgctgt atatgtcaag gtgttctgtc ttacaccaaa
     6301 tattgtgaat gtaaaggtat tcgttactga aacgaagcag gtatgtgttg acaacagtca
     6361 gttgcaaggc acaccggtaa gtattgcgaa tgtatgtgac aatctttcgg gcgagtttag
     6421 tctggtgaat attacaagta caggttattg tgtaaatatc acaggaatag agccaggaat
     6481 agagaaggca tgtattgtag tgtgcgatga ccttggcttc tgtgatacca catatatcaa
     6541 catcaccgtg tatgaaacca acctcaagcc gatagccgta gatgattatt tgactgcaag
     6601 tgcaggtaaa tctaaaacag tacaagtgct tgcaaatgat attttcgcag atggtttgct
     6661 cagcatgaaa ataatttctt ctcctactac cggaaaagtg acttcattca tagataaagt
     6721 tgttgtggtg acaccagata aggaggattg ccagccaatg gtatttgatt atgaaatatg
     6781 caaccagtat ggatgcgata cgggtaccgt gcatgtcaat gtactctgta aaggactaat
     6841 catctacaat ggtatgacgc ctaataacga tggattgaat gattatttca ccattgatgg
     6901 aatagaagat tatccgaata atgaattgtc ggtttataac cgttggggca acatggtata
     6961 ttctgccaaa gcttataaca ataattggga tggtagctgg aatggtaaac tacttcccga
     7021 tggtacctac tattacattt tgaaagatgg cgaaggtgaa agctataacg gctatcttca
     7081 aatatcgaga tgataaatga ttgataatta gccggtttga tgaaaaccgg cttttttgta
     7141 gataaaattt taagtttttt ttgaaaatat tgtccaagta ttcttggata ttaaaaaaaa
     7201 gaaagtaatt ttgcatcgcc tttcgattaa ggctatgatc tcgtagctca gccggtagag
     7261 catttgactt ttaatcaaag ggtctcgggt tcgaatcccg acgagatcac tccaaaaagc
     7321 atcagattat catctgttgc tttttttgtt atgtagctct ctgaaaatta tgaagaagcc
     7381 tagtgggaaa aaagaagctg atggttatgg tgatagctga acccacggca atctttttta
     7441 ctttctagtt attgcaccaa agttgtagaa aactattcat taggcaagta ctgatgtctg
     7501 tattgccctt gatcaatttt gaatgtttga agcgctttgc ttaaaatgct ataaaatcaa
     7561 ctgaggagca cataagtaat ccagctgccg aaataagcaa gtgcgctcat gtagataagt
     7621 tgtatgagtg gccatttcca tgtacctgtt tcttttcgta ctacagcgag cgtgctcata
     7681 cattgcatgg caaagaggta aaagattagt aaactcatag atgcagctat tgtaaaggtc
     7741 ttttgaccgg tattggcatt tatttcctta gacattcttt tttgtagcgt ttccgggtct
     7801 tcatcgccag agcctatact gtaaatggtg gacatggtgc caatgaatac ctcacgtgct
     7861 gcaaaagatg taatgagtgc aatgcctatt ttccagtcaa atccaagcgg aacaatagca
     7921 ggttcaatga atttgccgat atggccggcg tatgaatttt ctattttggc ggatgctttt
     7981 aggttttctg tttcttcagg tgtcatgttt tgactcgcag catattctgt tgcttgtttt
     8041 tgagctgatt ctatctcatt tccagggcca aatgatgcca gaaaccaaag tgcaacactt
     8101 atgaccataa taatcattcc tgcctctgat atgaagcttt gtaccttact cttgacggcc
     8161 agcaaaatgc ttttccagtg aggtaccttg tattccggga gctcgatcat caggaagctg
     8221 gtttcgtttg tctttagcag ttttttaaac acaatggctg ctaccagtgc tgcaagtaac
     8281 cccagactgt agagcaaaaa aagaatcaaa cttcgcatgc caatggggcc gaaaaatgta
     8341 ttatcggtaa tgataaaact aatgagcacc gcaaagaccg gtaaacgagc cgaacagctg
     8401 ataagaggag tgaccattat agtgataagc ctttctttcc agttgttgat ggtacgtgca
     8461 ctcataatag ccggaatggc acaagctgca cctgatacta atgaaatgat gctacgacca
     8521 ttcagtccaa attttaaaag aaggcggtcg aacaggtaaa tagccctcgc catataccca
     8581 agttcttcca gaaaactcgt aaacaagaat aagatagcaa tctgcggtat aaacaccaat
     8641 acaccaccta agccgggcaa tatgccatca accagcaact cggtaaacca tcctgccggc
     8701 aaatggttct gtacaagttc tgttagagca attatgccgg tttcgaacat ttgcatcggc
     8761 cactctgccc agctgaatac agcctggaaa atcaagagta ggatagagat aaatatcaat
     8821 ggcccccaaa cacggtgtgt aagtatgcgg tcaagtgtgt gggttttatt tttaccatcg
     8881 gcagttatca ggctttggtg cttcacaagg ctgtgttcta tccagcgata tctttgcatg
     8941 gtttcgtcaa tctgtgaatg cagactgtca aatcccaatt tcgatgtttg ttcttttata
     9001 tattccttct gctcttcgtt caggaattgg agctcctcaa cgtggtgtgc agtcagaagt
     9061 gcctgaaact tagtttttgc accggttttg ctgacgattt tttctgataa ttcatttgtt
     9121 ttagcatccg gcacataagt ctgaaaatta ttggtcggat gttcgtttaa cgaacacatt
     9181 tcagaaattt tggtctttaa ttcgttaaga ccatgacctg tacggccatt gagccagacc
     9241 accggtgtgc caaaggattt ttcaaggcct ttagcatcaa ttttgatttt gcgtgcctct
     9301 gccagatcct gcatattgag cccaagtatg attggtacac caagatcttt gagttgggta
     9361 aagagcaaaa gatgccgctc gagttgggtg gcatccgcaa tatagactat caggtctggg
     9421 aagagattat cgttcgggtt ggcgagaatt ttgaaaacga ctttttcgtc gtaagcggta
     9481 ggatagaga
//
LOCUS       DAOQQY010000081         9440 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2710_length_9440_cov_6.239744, whole genome shotgun sequence.
ACCESSION   DAOQQY010000081 DAOQQY010000000
VERSION     DAOQQY010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_9440_cov_6.239744"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1525)
                     /locus_tag="PLE23_05535"
     CDS             complement(<1..1525)
                     /locus_tag="PLE23_05535"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HMX82761.1"
                     /translation="MKKGKLLLALLWTLLCINEGAGQKMIEGYRYGDEQMPSGKEWES
                     PQDLALNKELPHAWFSSFQDVSSALKVLPENSKYWKTLNGTWKFHFAKTPDTRPKDFY
                     KLTFDVSGWDNVPVPLSWNIYGIQKDGSLKYGVPIYVNQPVIFQHKIEVDDWRGGVMR
                     TPPKEWTTYEYRNEVGSFRRDFDIPADWNGREVFLNFDGVDCFFYLWVNGRYVGFSKN
                     SRNAATFNIQKYLKPDKNTLAVEVYRLSDGSFLESQDMFRLPGIFRTVSLYSVPKLHI
                     RDLRVTPDLDENYNNGSLNIQADVRNLSNDKAEKCNIEYTLYANKLYADETTKLANVS
                     ASASLTDILPKEIKTTVNAVIKVDNPNKWSAEEPYRYTLVAELKDSKGKTIETVSAIV
                     GFRKVEIKDTQAEKDEFELAGRYYYVNGKTVKLRGVNRHETNPSVGHAITREMMENEI
                     ILMKRANINHVRNAHYPDDPYWYHLCDKYGIYLEDEANIESHEYYYGDASLSHPVEWK
                     "
     gene            complement(1561..5427)
                     /locus_tag="PLE23_05540"
     CDS             complement(1561..5427)
                     /locus_tag="PLE23_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HMX82762.1"
                     /translation="MWVRRAIFVMNHHCTKIMTPNNFSKRVLIALPAFFLYLLLPAQV
                     ELKNFTGGKLTGTHEAELVFGNQQVLYIDFYSENSFRMFYSPHGDKPHVPVAKPPAKI
                     LADNPRKPVGALQISDAGNEISIQSKKIKLTIDKKSGILTAFNLAFNKQAFKLNKPIF
                     VENDKSNIALSEGTDEYFYGGGVQNGRFSHKGKAISIENQNSWTDGGVASPVPFYWST
                     SGYGLLWHTFKPGRYDFGLKERGTVRLFHNDSYLDVFIMISQEPVQLLQGYYQLTGQA
                     LLLPKFGFYQGHLNAYNRDYWKEDTTGKRGIRFEDGKYYTESQKDNGGILESLNGEKN
                     NYQFSARAVIVRYRQHDMPLGWILPNDGYGAGYGQTSTLDSNIQNLKSFGDYARKNGV
                     EIGLWTQSDLHPKQGISALLQRDIVKEVRDAGVRVLKTDVAWVGAGYSFGLNGVADAA
                     SVFAEYGSGARPFIISLDGWAGTQRYAGVWSGDQTGGNWEYIRFHIPTYIGAGLSGMP
                     NITSDMDGIFGGKNLPVNVRDFQWKTFTPMQLNMDGWGTNEKYPHALGEPATSINRLY
                     LKLKSELLPYSYSIARQAVNGLPMIQAMFLVDPNEFTLGTATQYQYMYGPSFLVAPVY
                     QNTAADAQGNDIRNNIYLPPGSWVDYFTGEEYEGGKVINSFSVPLWKIPVFVKAGAII
                     PLANANNNVSEIDKKHRIYEFYPKGNTSFTEYDDDGISQEYLSGKYTTTQIESKEANN
                     KAELIIHPTVGDFKNFTHIKTTEFKVNAPILPNHIEAKINAKKIKLKQVYTEAEYKSG
                     ENVWIYLQPEFNRYATKGSDFSQKIIKRNAQLWVKLEPGDVSRTTLTLSIKKFRQEPK
                     HKTTLRSEGPEKPIAEMKEENRHAFSLLPSWKAVPGADYYEIEFNGMLYTLIRDTTFL
                     FEDLQPETSYRFKIRAKNITESPWTVIETKTLANPLQDAIHGLTARVSVRNQPGEGIA
                     NLFDFDEANIWHTRWDAPAVPFNMIINLNSISQIEKLQYLPRTGAGNGTWLKGNISYS
                     IDKANWLQAGTFTWKRDDKVKEFSFAEKPVTRYLKIEVSEAVGNFGSGRELYVYRVSG
                     TESQLPGDINHDGLIDQNDLTSYMNYTGLRQGDADFEGYISKGDLNRNGVIDAYDISN
                     VATALEGGAWEESQDTMSGKILLQNDKANYSKGDTMHITVSSAGFSLVNALGFALPYN
                     TADYEYAGIQAIHTGNLENFTRDRLHTDGNKVLYPVFVNIGDRQTLSGSDKLFVIRFV
                     AKRNLTFGLKAKDGLLVDKRLIQLKF"
     gene            complement(5498..6331)
                     /gene="fdhD"
                     /locus_tag="PLE23_05545"
     CDS             complement(5498..6331)
                     /gene="fdhD"
                     /locus_tag="PLE23_05545"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HMX82763.1"
                     /translation="MNRNIHTRKIHKLTPENLLATDDFLAVEEPLEIKIVYGPKENRI
                     SKTISITMRTPGEDRELALGFLFTEGIINQPADILATESTIPSFLNQNTIEIQLSDDA
                     NPDIHRLDRHFYTTSSCGVCGKSSIDALKTIRMPKLKADFPVFDRNVILSLPSLLTQL
                     QSVFEKTGGIHASAIFDKSGKLLLLKEDVGRHNALDKIAGAALQQGLLPLSEQILLVS
                     GRASFELVQKAAMIGVPLMAAVGAPSSLAAELAEEAGITLVGFLNGKRFNIYTHPGRI
                     L"
     gene            complement(6431..7930)
                     /locus_tag="PLE23_05550"
     CDS             complement(6431..7930)
                     /locus_tag="PLE23_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMX82764.1"
                     /translation="MGWKSRIIYRFAKYVAADIRKWSANAVFTQESIFKRLIERSVNT
                     DFGIDHNFRNIRNYEDFQNQVPVRDYEGLKPYIEKIKQGGESVLWPGVPAYFAKTSGT
                     TSGVKYIPLTKESLPNHFGSARNAIFNYYGTTGKGTFFDGNLIFLSGSPELEKVGNIL
                     TGRLSGIVNHQVPSWLRTNQKPSYKTNCMEDWEEKVNAIVEETIHADMRLISGIPPWV
                     QMYYERLLERSGKKYIKDIFPNYSMFVYGGVNFEPYRSTLEELVGKRIDSVETYPASE
                     GFIAFQDKQDSKGLLLNTNSGIFFEFIPVSEFGSDNATRYSLADVETGVNYVLIINNN
                     AGLWGYNIGDTVEFVSKNPYRIVVTGRVKHYISAFGEHVIGKEVEDAMLRACQQVHAK
                     VSEFTVAPQINPGNDEKPYHEWFIEFDEMPVDLKKFEACLDAEMVRQNIYYEDLITGA
                     ILQSLKLRVLKKDAFRNYMKSQGKLGGQNKVPRLSNDRKLADELKDFII"
     gene            8240..>9440
                     /gene="radA"
                     /locus_tag="PLE23_05555"
     CDS             8240..>9440
                     /gene="radA"
                     /locus_tag="PLE23_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMX82765.1"
                     /translation="MAKLRKIFVCTECGSNSPKWIGNCPTCGAWNTYIEEIAGNKTAQ
                     ESKSATWRVSSPKDFEIKPIPLTEISPGRLVRTDTRDNELNRVLGGGIVPGSLVLIGG
                     EPGIGKSTLSLQMATQIGIKVLYVSGEESEDQIKLRADRLSGNTPECFIYTETNTSKM
                     LNLAASLQPGLMIIDSIQTISSPNIESMPGTISQVRECTAELQRFAKETNIPVIIIGH
                     ITKEGSIAGPKILEHIVDTVLQFEGDRQHSYRILRTLKNRFGSTDEIGIYEMQAGGLR
                     QVCNPSELLLTQRDDHLSGSTVSATMEGMRPVLIETQALVATAIFGTPQRSSTGFDLR
                     RLHMLLAVLEKRSGIFFGQKDVFLNIAGGLKIVDTAVDLGLVAALVSSLHDIYVSQKV
                     CFAGEIGL"
BASE COUNT         2422 a         2079 c         1982 g         2957 t
ORIGIN      
        1 ccttccattc tacaggatgt gataaggatg catcaccgta gtagtattcg tgcgactcga
       61 tattggcttc gtcttccaga tatatgccgt atttgtcaca cagatggtac cagtacggat
      121 catccggata atgcgcattt cttacatgat tgatattggc acgcttcatc agaataattt
      181 cgttttccat catttcacgc gtaatcgcgt gccctaccga agggtttgtc tcatgcctgt
      241 tgacgccgcg cagtttcact gttttgccat ttacataata atatcttcct gcaagctcga
      301 actcatcttt ttctgcttga gtgtccttga tttcgacctt tcgaaaacct acaatagccg
      361 acaccgtttc aatcgttttg cccttactgt ctttaagctc tgctacaagt gtatagcggt
      421 atggttcttc agccgaccac ttattagggt tatcgacttt tatgactgca ttgacagtag
      481 tcttgatttc tttgggtaaa atgtctgtca atgatgccga tgccgataca tttgccaatt
      541 tggtagtttc gtcagcatac aatttattgg catacaatgt atattcaatg ttgcactttt
      601 ctgccttatc attactcaga tttcggacat ccgcctgtat gttgagtgaa ccgttattgt
      661 aattttcatc caagtcgggt gtaactctca gatcccttat atgtaatttc ggaacggagt
      721 acagcgagac tgttcggaaa attcccggca gtcggaacat atcctgcgac tctaaaaacg
      781 acccgtcgga caggcgataa acttctacag ccagtgtatt tttgtccggt ttcaggtatt
      841 tttggatgtt gaatgtggct gcattgcgcg aattcttcga aaagccaacg tacctgccat
      901 ttacccaaag gtagaaaaag cagtcaacac catcaaaatt taggaatacc tcacgtccat
      961 tccaatctgc cggaatgtca aaatctctcc tgaaggatcc tacctcgttt ctgtattcgt
     1021 aagttgtcca ttcttttggt ggtgtgcgca ttacaccgcc gcgccagtca tccacttcta
     1081 tcttgtgttg aaaaatgaca ggttggttta cgtatatcgg cacgccatat ttaaggcttc
     1141 cgtctttctg gatgccgtag atattccagc tcaatggtac gggcacattg tcccagccag
     1201 atacatcaaa agtaagtttg taaaaatctt tcggtcttgt atcgggtgtt ttggcaaaat
     1261 ggaatttcca ggtaccgttc agtgttttcc aatattttga gttttcgggc aataccttca
     1321 gggctgatga gacatcctga aatgagctaa accaggcatg aggcagctct ttgttcaatg
     1381 ccaaatcctg aggcgattcc cactcttttc ctgaaggcat ctgctcatca ccatagcggt
     1441 agccttctat cattttctgg cctgcccctt catttataca tagcaaagtc cacaatagtg
     1501 ccaataataa ctttcctttt ttcataaaat atcccaatta ctttttatat ttattactta
     1561 ctaaaacttc aactgtatca gtctcttatc taccagcaga ccatcttttg ctttaagccc
     1621 gaatgtaagg ttccttttag caacaaaccg gatgacgaac agtttgtcgc ttccgctcaa
     1681 tgtctgcctg tcacctatat tgacaaacac aggatacagt accttattgc catcggtgtg
     1741 aagacggtcg cgtgtgaaat tttccagatt tcctgtatga atggcctgaa tacctgcata
     1801 ttcataatct gcagtattgt atggcaaggc aaaaccaagg gcattgacca agctgaatcc
     1861 ggctgaactc accgtgatat gcattgtatc acctttgctg taatttgcct tatcattttg
     1921 cagtaatatt tttcctgaca tggtatcctg actttcttcc cacgctccgc cttcaagtgc
     1981 tgtagccacg ttcgatatat cgtatgcgtc aatgacaccg ttgcggttta ggtcgccctt
     2041 gctgatgtag ccttcaaaat cggcatctcc ttggcgcagt cctgtataat tcatgtacga
     2101 agtcaggtca ttctggtcta tcaatccgtc gtgattgatg tcgcccggta gttgcgactc
     2161 cgtacccgat accctgtaca catatagctc acggccagag ccaaaattgc caacagcctc
     2221 tgatacttct atctttaagt atcgtgtcac cggtttttct gcgaatgaaa attccttcac
     2281 tttatcatct cgcttccagg taaaagtacc tgcttgaagc cagttggctt tatctatgct
     2341 gtaagaaatg tttcccttga gccatgtgcc attgccggct ccggttctag gcaaatattg
     2401 caatttttcg atctggctga tgctgttgag attgatgatc atgttaaacg gcactgccgg
     2461 tgcatcccaa cgcgtgtgcc aaatgttcgc ttcgtcaaaa tcaaataaat tggctatgcc
     2521 ttcacccggt tggttgcgga cacttactcg tgcggttaag ccatggatag catcctgaag
     2581 cggattggca agcgtttttg tctcgattac tgtccatggc gattcggtaa tattttttgc
     2641 tctgatcttg aaacggtagc ttgtttcggg ctggaggtct tcgaacagga aagtcgtgtc
     2701 acgtatcagt gtgtataaca tgccgttgaa ttctatttca tagtaatctg ctcccggtac
     2761 agctttccac gaaggcaaca ggctgaatgc atgccgattt tcttccttca tctccgcaat
     2821 tggtttttct ggtccctctg agcgtagagt ggttttatgt tttggttcct ggcgaaactt
     2881 ttttatactc agagttaagg tagtccggct tacgtcacca ggctcgagtt ttacccacag
     2941 ttgagcattg cgtttgataa tcttttgtga aaaatcactt cccttagttg cgtagcggtt
     3001 aaattcgggc tgaagataaa tccagacatt ttcacctgat ttatattctg cttcggtata
     3061 tacctgtttc agttttattt tctttgcatt gattttcgct tcaatatggt tgggtaatat
     3121 gggtgcattt actttaaatt cagttgtttt tatatgagtg aaatttttaa aatcaccaac
     3181 tgtgggatgg ataatcagtt cggctttatt gtttgcctcc ttgctttcaa tctgtgttgt
     3241 tgtatatttt cctgaaaggt attcttgcga aatgccatca tcatcatatt cggtaaatga
     3301 agtatttccc tttggataaa attcgtaaat acgatgtttt ttatcaatct ctgacacgtt
     3361 attatttgca ttggccagtg ggatgatagc ccctgcttta acgaaaacag gaattttcca
     3421 caatggtaca ctgaacgaat tgatcacctt gccgccttcg tattcttcac cggtaaaata
     3481 atcgacccag ctaccaggtg gcagataaat gttattgcgt atgtcattgc cttgggcatc
     3541 agcagccgta ttctgatata caggagcaac caagaaggaa ggtccgtaca tatattggta
     3601 ttgtgttgca gtaccgagtg taaattcgtt tggatctaca agaaacatag cctgtatcat
     3661 gggtagtccg tttactgcct gccttgcaat gctgtaacta tatgggagga gttcgctttt
     3721 tagtttgagg tagagcctgt tgatggaggt agccggttcc ccgagtgcat gtgggtattt
     3781 ttcgttggtg ccccagccat ccatgttcag ttgcatcggt gtaaatgttt tccactggaa
     3841 atcccggacg ttaaccggaa ggtttttacc tccgaatatg ccatccatgt ctgaagtgat
     3901 attaggcata cctgacaatc ccgcaccgat gtaggtaggt atatggaatc ggatgtactc
     3961 ccagttaccg cccgtctgat cacccgacca tacgcctgca taacgctgtg tgcctgccca
     4021 gccgtcaagc gatatgatga atggccttgc accagagccg tattctgcaa agacagaagc
     4081 agcatctgcc accccattga gcccgaatga ataaccggca cctacccaag ctacatctgt
     4141 cttgagtaca cgtacaccgg cgtctcttac ctcctttaca atatcacgct gaagcaatgc
     4201 actgatgcct tgtttaggat ggagatcact ttgggtccat aagccgattt ctactccatt
     4261 ttttctggcg taatcgccaa atgacttaag attctgtata ttgctgtcaa gtgttgatgt
     4321 ttggccgtag ccggctccgt agccatcatt tggcaatatc cagcctaacg gcatatcgtg
     4381 ttgcctgtac cggactatca cagcacgagc cgaaaactgg tagttgttct tttcaccgtt
     4441 caatgattca aggataccac cgttgtcttt ctgactttcg gtgtagtact tgccatcttc
     4501 aaaccgtatt cctcgttttc cggtggtatc ttctttccaa taatcgcggt tgtaagcatt
     4561 caggtggcct tggtagaatc cgaattttgg caataaaagt gcttgccccg taagttggta
     4621 atagccctga aggagctgta caggttcctg gcttatcata ataaatacat cgagataaga
     4681 atcattgtga aagagcctga ccgttcctcg ttcttttaag ccaaaatcat acctgcctgg
     4741 cttgaatgta tgccataaaa gcccgtagcc tgaagtactc cagtaaaatg gtaccggcga
     4801 agccacacca ccatcggtcc agctgttctg gttttctatt gaaatggctt tgcctttgtg
     4861 cgaaaagcgt ccgttttgaa caccaccacc ataaaaatat tcatctgtac cttcagatag
     4921 cgctatgttt gacttgtcat tttctacaaa tatcggctta tttagcttga aagcctgttt
     4981 attgaaagca aggttgaagg cagtaaggat tcctgatttt ttatcgatag tgagttttat
     5041 tttttttgac tgaatggata tttcattacc agcatctgag atttgcaatg ctcctacggg
     5101 ttttcttggg ttatctgcca ggatttttgc aggtggtttt gctacaggaa catgtggttt
     5161 atcgccgtga ggactataaa acatccggaa ggaattttca ctataaaaat cgatatatag
     5221 tacttgttgg ttaccaaaaa cgagctctgc ctcatgtgtt ccggtcagtt tgcctccggt
     5281 aaaatttttt aattctactt gagccggtag cagtaggtaa agaaaaaatg ccggcaaagc
     5341 aattaaaacc cgcttagaaa aattattggg tgtcattatt ttggtgcaat ggtggttcat
     5401 tacaaagata gctcttctta cccacatttt ctgacagaaa ttttcaagat ctgtctgtgt
     5461 tctttatgaa taatatttaa acattttacc gtaaatacta caagatcctt cctggatgcg
     5521 tataaatatt aaatcgtttc ccgttcagga atccgacaag ggtaattcct gcttcttcgg
     5581 ctagttctgc tgccagacta gagggtgccc ctacggctgc catcaatggc acacctatca
     5641 tagccgcttt ttgtaccagt tcaaaacttg ctctgccgct tacgagtagg atttgctctg
     5701 acaatggtag cagaccttgt tgcaatgctg caccggctat cttatccagt gcattgtgtc
     5761 ggcctacatc ttctttgagt aacagcagct tgcccgactt gtcgaagatg gctgaagcat
     5821 ggatgccacc ggttttttcg aatacacttt gcagttgtgt cagcaatgag gggagactga
     5881 gtatcacatt tctgtcaaaa accggaaaat ctgccttcaa cttcggcatt cttattgtct
     5941 tcagggcatc aatggacgac ttaccacaga caccgcaact cgatgttgta taaaaatgtc
     6001 gatccaacct gtggatatca ggatttgcat catctgacag ttgtatttca attgtatttt
     6061 ggttgagaaa tgaaggtatg gtactttccg tagcaagtat atcagcaggt tggtttatga
     6121 taccttcggt aaataagaaa ccaagagcga gttcgcggtc ttctcccggt gtgcgcatgg
     6181 tgatcgaaat ggttttgcta attcggtttt cctttggtcc atacactatt tttatttcga
     6241 gcggttcttc gactgcaaga aaatcatctg tagcgagtaa attttcagga gtcagtttgt
     6301 gaatttttct ggtatggata ttcctgttca tttggtgaaa ttttgagtgt gtgctttccg
     6361 aatttcattc aatattgatt tattggacgt ttaacaaatt ggacagctat tgtcttggat
     6421 cattgttttg ttatatgata aaatctttca gttcatcagc cagtttgcgg tcgtttgaca
     6481 gtcggggtac cttgttctga cctccgagtt tgccctgcga tttcatataa ttcctgaaag
     6541 cgtccttttt tagaactctc agtttcagac tttgtaaaat agctccggta atcaggtctt
     6601 cgtaatagat attttgcctg accatttcag catccagaca tgcttcgaac ttcttaagat
     6661 caaccggcat ttcatcaaat tcaataaacc attcgtgata gggtttttca tcattgcccg
     6721 gattgatttg tggtgctaca gtaaactctg ataccttggc atgtacctgt tggcaagccc
     6781 tcagcatggc gtcttcgact tcttttccta tgacatgttc gccaaatgct gaaatataat
     6841 gttttacacg tccggtgacg acgattctgt atggattctt gcttacaaac tcgactgtat
     6901 cacctatgtt gtagccccaa aggccggcat tgttgttgat gatgagtaca taattgactc
     6961 ctgtctctac atctgcaagg ctgtagcgcg tagcattgtc agagccaaac tccgacaccg
     7021 gaatgaattc gaagaaaata ccggaattgg tattcaaaag tagcccctta ctatcctgct
     7081 tgtcctggaa agctataaat ccttctgatg caggatatgt ttctacgcta tcaatgcgtt
     7141 tcccaaccag ttcttcaagc gtacttctgt atggttcgaa gttaacacca ccgtaaacaa
     7201 acatagaata atttggaaaa atatccttga tgtatttctt gccagaccgt tcaagcaagc
     7261 gctcataata catctgaacc cagggtggaa tgccgctgat gagccgcata tcggcatgga
     7321 ttgtttcttc tacaatggca tttacttttt cttcccagtc ttccatacaa tttgtcttgt
     7381 acgaaggctt ttggttggtg cggagccatg aaggtacctg atgattgact ataccagaaa
     7441 gacggccggt gaggatgttg cctacctttt caagttcagg acttcctgac aggaaaatca
     7501 aatttccgtc aaaaaatgtt cccttacctg ttgtaccgta gtaattgaat atggcattgc
     7561 gtgctgaacc aaaatgattg ggcaggcttt ccttggtaag cggaatgtac tttacgccgg
     7621 aggtggttcc gctggttttg gcaaaatagg caggtacacc cggccaaaga accgattcac
     7681 cgccttgttt aattttttct atataaggtt tgagcccttc ataatctcgg acaggtacct
     7741 gattttgaaa atcttcgtag ttcctgatat tcctaaaatt gtgatcaata ccgaaatcgg
     7801 tatttactga tctttcaatg agtcttttga atattgattc ctgagtaaat acggcattgg
     7861 ccgaccattt gcggatatcg gcagccacat actttgcaaa gcgataaatt attctcgatt
     7921 tccaacccat agtgtaaata tatgagtttt gtgaaaaatg gaaacaatat ttaatcgctg
     7981 aaaatatttt ttagcttatt tatcgaaaaa actcagggca ttcaatcaaa tcacttcaat
     8041 cgatgccctg cataacccca aaaaaccaaa tgaaaatatg tctttatagc gtgctggttg
     8101 tctgctatct gataatatga cgcacggcta gtgtaaatgt cacaaaattt taacaacaaa
     8161 aattttaaga caaagaattt aattaatttt gaacctttac gccagaattt taattaaata
     8221 ttaccctgta atttacatta tggccaaact ccgcaaaata ttcgtctgta ccgaatgtgg
     8281 cagcaattcg cctaaatgga tcggcaactg tccaacctgt ggggcatgga atacttatat
     8341 cgaagagata gcaggtaaca agacagctca ggaatcaaaa tcagctacct ggcgagtttc
     8401 ttcacctaaa gattttgaaa taaagccgat tccacttaca gaaatttcac ccggcaggct
     8461 tgtcagaacc gacacgcgtg acaatgagct gaaccgcgta cttggtggag gcattgtccc
     8521 cggttcgttg gtgcttatag gcggggagcc gggcattgga aaatcaacac tttcattgca
     8581 gatggccaca cagattggca ttaaagtgtt gtatgtgtca ggtgaagaaa gcgaagatca
     8641 gataaagctg cgtgctgacc ggctttccgg caatactcct gagtgtttca tttatacaga
     8701 gaccaatacg tctaaaatgc tcaacctggc agcatcactc caacccgggc ttatgattat
     8761 tgattctatc caaaccatct catctccaaa tatcgaatcg atgccgggca ccatttcgca
     8821 ggtacgcgaa tgtacagccg aattgcagcg ttttgccaag gaaaccaata taccggtcat
     8881 tattatcggg catattacca aggaaggcag cattgccggc ccaaagatat tggaacatat
     8941 tgtcgatacc gtactacagt tcgaaggtga cagacagcat agttaccgga tattgcgcac
     9001 gctcaagaac cgatttggca gcacagacga aatcggaatt tacgaaatgc aagcaggagg
     9061 cctcagacag gtatgtaatc cgtcagagtt gctcctaacc caacgcgatg atcacctgag
     9121 tggcagtact gtttcggcca ctatggaagg tatgcgccca gtattgatag agacacaggc
     9181 actggtggca acggctattt ttggcacgcc ccaacgtagc tccacaggct tcgatctgcg
     9241 aagactacac atgctcctgg cagtacttga aaaacgaagt ggtatatttt tcggtcagaa
     9301 agacgtattc ctaaacattg ccggtggatt aaagattgta gatacagcag tcgatctggg
     9361 cctggtagca gctttggttt cttcactgca tgacatttat gtgtcgcaga aagtgtgctt
     9421 tgcgggagaa attggcttgt
//
LOCUS       DAOQQY010000082         9273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2791_length_9273_cov_6.312866, whole genome shotgun sequence.
ACCESSION   DAOQQY010000082 DAOQQY010000000
VERSION     DAOQQY010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2791_length_9273_cov_6.312866"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLE23_05560"
     CDS             complement(<1..639)
                     /locus_tag="PLE23_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMX82766.1"
                     /translation="MQLVGENYQLTGEIDPLYLVNKYGAPLYVYDTSIIASQYKRLST
                     AFDVPSLRINFACKALTNLAILKFMNSLGAGLDTVSIEEVRLGIAAGFDPHNIIFTPN
                     CVSVEEIELAIEAGVRINIDNISTLEQIGTRHPGLPVCIRVNPHIMAGGNSKISTGHI
                     DSKFGISIHQMPHVLRIVDAIGMKIEGVHMHTGSDILDVDVFLAGAEILLKIA"
     gene            821..1348
                     /locus_tag="PLE23_05565"
     CDS             821..1348
                     /locus_tag="PLE23_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX82767.1"
                     /translation="MIPDYLIEACIALDPKAQKQLYEATRSRMYVLVLRYIREHHLAE
                     DVMIEAYVKVFKNIQKYRRDGAFEAWMRRIFVNECLMFLRKEKNIVFEPLTVLPDDSA
                     FEPPDEFDYEEIMLLIEKLPAGCKTVFKMYVFDDLQHNEIAQLLNISISTSKSQYQLA
                     RTKLLGMIKNKQEEN"
     gene            1418..2323
                     /locus_tag="PLE23_05570"
     CDS             1418..2323
                     /locus_tag="PLE23_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82768.1"
                     /translation="MKESLKKSLQEAEHRYSPKNVPEQWHLIEARLKGKEQTGNIDRS
                     VWFKIAATMLLLIIAVFIVKFYLQKNQKDWAQTLTTPASELDTLAESPENESLKLPSA
                     PEQSMEIPSKKETESKGIRQMQGAGNNKHTTDAPATVAMADHIEEQESEVSRSMAEPT
                     PASAAPPQAARQKEATGKAEVQADKAAVKNTAHTFAGKYDGIPFVASVKNTEAGWVLV
                     GQTQNTPHKKYRFVKEKQSLECIDSGGKNVFQYSSTDTKGEHYTSADGKITVRNTDES
                     IQITMIIEKPGGPLSHYWELYRTYE"
     gene            complement(2327..3559)
                     /locus_tag="PLE23_05575"
     CDS             complement(2327..3559)
                     /locus_tag="PLE23_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82769.1"
                     /translation="MLQASKNSNTIQTTILNGTGFSMQVVESIAGVAEEWDNILEGNN
                     TLLSSAYLMALELYPPFGMAFRYLVLRDLQGCGVGVAYFQIQHFNAANSLNEADDKKS
                     PWLIGSLQKYVKDIVARNVVFNTFVFGNLLLTGENAFYFRKNIMPDDQIINLMTAAAE
                     SLQLTLNSAGIQCNVCLMKDFKAIEPNQTRNLLSKKNFHEFCVQPCMMMSIQDEWLSF
                     DDYLGSLHSKYRVRAKRAFKKRQELSCRELGQDEILLYQNQIHDLYMNVAQNAGFNTV
                     NLHVGYFYGLKKYLGDKFQLFAYFRGNVIKAFYTIIHNGHELEAHFLGYDKQENAISQ
                     IYLNSLFDIIQHAIEGRFSLINFSRTALEIKSSVGAVPLDMYCYMKHRSHIPNKFIKP
                     IIDYLNPDFDWQPRRPFK"
     gene            3871..4839
                     /locus_tag="PLE23_05580"
     CDS             3871..4839
                     /locus_tag="PLE23_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82770.1"
                     /translation="MKKYFFLLLFLPFIVSSCVSSKKYNKLQFERDQLKYNYDQLVKV
                     NAENRILLTKIDSLQLLSDRLNSELKHAKEAAASAQKAFDDVTVRYNKLVEQNKMLTN
                     ASSQERGKFINELNEKDKLLTTREQQLDSLSNVLTNKQKALDLATKNLEEREQKLREL
                     QRIIDEKDQNLAGLQNKLKGALSGFSPEDLNIVQKDGKLYATLSQDLLFASGSKQIDP
                     KGIDALKKFGGVLKNYPDIEVSVEGHTDTDGSAELNWNLSADRAISVTKVLIGEGVDA
                     KRVIPSGRAFYVPVASNDTKEGKGKNRRTEIILSPKLTALYNLINK"
     gene            4847..6040
                     /locus_tag="PLE23_05585"
     CDS             4847..6040
                     /locus_tag="PLE23_05585"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMX82771.1"
                     /translation="MKNLFSVVFTLIATTAFSQGVRCGTVFSGKLDELFISHAPVSRS
                     VITIPVVFHIVWRDSSDNIPDARITSQLDVLNTDFRALNDQLKYLSSAKRSLAADMEI
                     EFCLAAVDPQGIPTTGILRYKSEVENIGTQNTPTIPSKAFIKHKNLGGADAWDTQRYL
                     NIWVGKFQDGAILAESKFPWDNLPEEDGIRIDPAYVGVNCNGSPKKQFALGRTLTHEV
                     GHYLGLLHPWSADCATGDYVEDTPPQRCPYYGCMPQVMDDACGSVPLVENFMQFSDDA
                     CLALFTHGQKERVYQMLLQYRPLLLNNNISCFKPTFADRLTEDNVRIYPNPTSHCISI
                     DLRLNRESDIKVEIFDTSGKLIWKDVVKVLLLRPIELSFTQSGIYFIRLTGNENTLVR
                     KVVVM"
     gene            complement(6059..8728)
                     /locus_tag="PLE23_05590"
     CDS             complement(6059..8728)
                     /locus_tag="PLE23_05590"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HMX82772.1"
                     /translation="MNTRIIYNFFLYLTFALPGMLVAQQNPGVVLRPDSLGIVSADTS
                     GTPIRDSVLVSYTDFKLSKDSLDAEVDCSAKDTIWYDINNKRIHLINKAWMKYKTIDL
                     KANYIIFDWNTNVLSAMGRPDSLGKMTGFPDFADGDQKFKSKELKYNFKSKKGLAKDI
                     TSKENDLFVHGETAKFLVAKDSLHKQTIVYNKNALITTCDHPDPHYGIRSSKQKVVAD
                     KVAVVGPSFLEINHIPTPVVLPFGFFPITKGQRAGLILPRDYENSETWGIGFRDIGYY
                     FPIGKHFDVKLTGDIYFRGSWGVRANTRYVQRYKYNGGVNMAFSDRRSEIPGSNKIAS
                     DKSFSFIWQHNQDPKAHPSITFGGNVNIQSNNFQSLNYNDANSVLNNTLSSNINFSKR
                     FPGTSQVLTASLSHSQNNISREVIINFPNIGYQTGSIYPLKRKAQSIGPERWYEKIYF
                     TYDMQAKNEIKTLDTLLFRPETLRNSKYGIKQSISTSYNTRILKYVNVAPSIRYEETW
                     NFQTLKKGFDETITIDTIPVKNQAGEITDYTYKTNYGKILSKTVHGFAPFRTFTASLS
                     ANTQLFGSLQFRKGFLRGVRHVIKPSISLNYSPDYASIDRYFGTINTDSRPEFNRMQR
                     YSIFESGIFGTPPNSKQSLNLAYGINNLFEGKYYHKRDSTIKKFKFFDNINIGGSYNF
                     AADSLNWSRLSYGGTARFFKGITTLTISGSFDPYDVDAYGRTINKLYYKSKGKLLRFV
                     NTNFSFNSAISLKQIWEIQEDRQNAGGPQSVSVPQELLQNIFIRHTLNIGIYPLQQGD
                     TIAIVNHNLDLNGSLPITKNWRIDVGNIGWDFKAKRLTYPDIGFYRDLHCWEMGMNWQ
                     PERGTYAFFLRVKPSSLDFLNIPYRKGFQDAQF"
     gene            8788..>9273
                     /locus_tag="PLE23_05595"
     CDS             8788..>9273
                     /locus_tag="PLE23_05595"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMX82773.1"
                     /translation="MPGTTHLKTTKHILRCYFLVVFNIFFLFHFLEAQPKQPQNNGKA
                     TQLQNNTKKKNLIRTIVIDAGHGGHDPGTVNFKKKEKDITLSIALKAGELIERNFKDV
                     RVVYTRKKDEFIDLKKRAQIANQERADLFISIHCNAIETNQENFYGTETYVQDPKVES
                     YN"
BASE COUNT         2796 a         1790 c         1991 g         2696 t
ORIGIN      
        1 ggctattttt aataaaattt cggcacctgc gaggaaaaca tccacatcaa gtatatcaga
       61 cccggtgtgc atgtgtacac cttcgatttt cataccgatg gcatccacta tacgcaatac
      121 atgtggcatc tgatgaatcg aaataccgaa tttggaatcg atatgtcctg tcgaaatctt
      181 agagtttcct cctgccataa tgtgtggatt gacacggatg caaaccggta ggcccgggtg
      241 gcgggtgcct atttgctcta atgttgaaat attgtcaatg ttaatacgaa caccggcttc
      301 gatagccagc tctatttctt caacagagac acaattgggt gtaaaaataa tattgtgcgg
      361 atcaaaccct gctgcaatac cgagtcggac ttcttcaata gacacagtgt cgagcccggc
      421 accaagcgag ttcatgaact ttaggattgc caggttggtg agtgccttgc atgcaaagtt
      481 tattctcaga gacggaacat cgaaggcagt tgacagtcgt ttatattgac ttgcaatgat
      541 acttgtgtca tagacgtaaa gtggcgcacc atacttattt acaaggtata atggatcgat
      601 ttctccggtt aactgataat tttctccgac taattgcatt tttcttgatt taatgtgcaa
      661 agataaagta aagatttatc tatacgtata tttatggctt ttgtgccatc attaaccaac
      721 cttttgtccg atttatgcat ctgattatgt acatacaggt atgttttggt acaagttttt
      781 gaatattttg aaatatcttt acactggaaa ttttcaaaaa ttgatacctg actatcttat
      841 agaggcttgc atcgcacttg acccgaaggc tcaaaaacaa ttgtacgaag ccacgaggtc
      901 gcgtatgtat gtacttgtac tgcgttatat aagagaacat caccttgctg aagatgtgat
      961 gattgaagct tacgtaaaag tgttcaagaa tattcagaaa tatcggcgag acggagcctt
     1021 tgaagcatgg atgcgccgga tttttgtaaa cgaatgtctg atgtttttga gaaaggagaa
     1081 gaatatcgtg ttcgaaccac ttaccgtatt gccagatgat tcagcttttg aaccacccga
     1141 cgagttcgac tatgaagaaa taatgctatt gatagaaaaa ttgcctgccg gctgcaaaac
     1201 agtgttcaaa atgtatgtgt ttgatgattt gcaacataat gaaattgctc aattgctcaa
     1261 cataagcata agtacgtcta agtcgcaata tcaactggca aggaccaagc tccttggcat
     1321 gataaagaac aaacaagagg aaaattgaat atgcacggat aaaattttat gaatattcag
     1381 cagtattgat tttaaataaa ttggttttga aaaataaatg aaagagagct taaaaaagtc
     1441 attgcaggaa gcagagcata ggtattctcc gaagaatgta cctgagcaat ggcatttgat
     1501 cgaagcgaga ctcaaaggca aggaacagac cggaaatatt gacagaagcg tttggtttaa
     1561 gattgcagcg actatgttgt tgttaatcat agcagttttt attgtaaaat tttatttgca
     1621 aaagaatcag aaagactggg cacaaaccct gactaccccg gcatcagaac ttgacacgct
     1681 ggccgagtca ccggaaaatg agtctctcaa gttaccttct gctcccgagc aatcaatgga
     1741 aatacccagc aagaaagaga ccgaatcgaa aggaatccga caaatgcagg gagcagggaa
     1801 taacaagcat acaaccgatg cgcccgcaac tgtagcaatg gcagaccata tagaagagca
     1861 ggagtctgaa gtatcacgca gtatggccga acccacacct gccagtgcag caccacccca
     1921 ggcagcaaga cagaaggagg caaccggtaa agcagaagtg caggccgata aagcagccgt
     1981 aaaaaatact gcacatacat tcgcaggtaa atatgacgga attccatttg tagcatcggt
     2041 caagaatacc gaagcaggtt gggtgcttgt aggccagacg caaaatactc cacacaaaaa
     2101 gtaccgtttt gtcaaagaaa agcaatcact tgaatgcata gattctggtg gaaaaaatgt
     2161 atttcaatat tcgtcaaccg ataccaaagg cgaacattat acatcagctg atggcaaaat
     2221 tactgtgcgt aacacggatg aatctatcca aataaccatg attatagaaa aacctggtgg
     2281 gccattatcc cattattggg aactataccg tacttacgaa taatcatcac ttgaatgggc
     2341 gccttggctg ccaatcaaag tcaggattga ggtaatcgat gattggtttt atgaatttat
     2401 ttggtatatg gctgcggtgc ttcatgtagc aatacatatc gagtggtact gcacctactg
     2461 agctttttat ttcaagagca gtacgactaa agttgattag gctgaacctt ccttcaatgg
     2521 catgttgtat gatatcgaac agactgttga gataaatctg cgaaatggca ttttcttgct
     2581 tgtcgtaccc aaggaaatgt gcttcaagtt cgtgcccgtt gtgtatgatg gtataaaatg
     2641 ccttaatgac attgccacga aaataagcaa atagctgaaa tttatcgcca agatattttt
     2701 taagaccata aaaatatcct acatgaagat tgacagtatt gaatcctgca ttctgagcta
     2761 cattcatgta caaatcatga atctgatttt gataaagcaa gatttcgtcc tgcccaagct
     2821 cacggcagct taattcttgc cgctttttga atgccctctt tgcccttaca cggtactttg
     2881 aatgcagact gccaagataa tcgtcaaatg ataaccattc gtcttgaatg gacatcatca
     2941 tacatggttg tacacaaaat tcatggaagt tttttttaga gagcaaattg cgagtttggt
     3001 taggctcaat tgctttgaag tctttcatca gacagacatt gcattgtatg ccggctgagt
     3061 tgagtgttag ttgtaatgat tcggctgctg ctgtcatcag attgattatc tggtcgtccg
     3121 gcataatatt ctttctgaaa taaaaagcat tttcacctgt aagcagaaga ttgccaaata
     3181 caaaagtgtt aaatacaaca ttacgtgcaa caatgtcttt aacgtatttt tgcaatgaac
     3241 caatgagcca aggacttttt ttgtcgtccg cttcgttcag actgttggca gcgttgaaat
     3301 gttgtatctg gaaataggct acaccgactc cacaaccttg aagatcacgc aacacaagat
     3361 acctgaaagc cataccaaac ggtggatata gctcaagagc catcagatat gccgatgaaa
     3421 ggagtgtatt gtttccttcc agaatattgt cccattcttc agctacaccg gcaatgcttt
     3481 caaccacctg catgctaaag ccggtaccat ttaatatagt agtttgaata gtgtttgaat
     3541 tttttgaagc ttgcaacatt taccaaaact aacatctaat aactttatat gtgctaaaaa
     3601 agttgtaacc ggaattgaat tatttgctca agctcgtgtt taacccaaat aacgcattag
     3661 gagtttaaac aaaaatgatt agtacaaaaa aatctaaaga aagtgcgaaa agtatagcga
     3721 attatgtttt ttggcttttg caatggattt gaactagtaa ttatttgcaa gaaagagatg
     3781 gatgtgttat ctgggtttaa taaattacca ttaaaaaaat cagtaatatg ttatattctc
     3841 actaactttg caactcctta tattttaccc atgaaaaaat attttttcct cctgcttttc
     3901 ctacctttta tcgtaagttc atgtgtttca tcgaaaaaat acaataaact acagttcgaa
     3961 cgcgatcagt taaagtacaa ttacgatcaa cttgtaaagg tgaatgctga aaaccggatt
     4021 ttgcttacga aaattgacag tctccagcta ttgtctgaca gattgaattc ggagctaaaa
     4081 catgcaaagg aagcggcagc gtctgcacaa aaagcatttg atgatgtgac agtacgttac
     4141 aacaaactcg tagaacaaaa caaaatgctg accaatgcca gctctcagga aaggggcaaa
     4201 tttatcaatg aactcaatga aaaagataaa ctgctcacca cacgcgaaca acagttggac
     4261 agcttgtcga acgtgttgac caacaaacaa aaagcactgg atctggctac aaaaaatcta
     4321 gaagaacgcg aacaaaaact gcgtgaattg caacgtatca ttgatgaaaa agatcagaat
     4381 cttgcaggcc tgcagaataa actgaaaggc gcactttcag gattttcgcc agaagatctc
     4441 aatatcgtcc agaaagatgg caaactttat gcaacactca gtcaggatct gctcttcgct
     4501 tcgggaagca aacagataga ccctaaaggc attgatgcgc tgaaaaaatt tgggggagtg
     4561 ttaaaaaatt atcctgatat agaagtaagc gtagaaggtc atacagacac cgatggttct
     4621 gcagagctta actggaattt gagtgctgac cgtgccattt cagtaaccaa agtcttgata
     4681 ggtgaaggag tagatgctaa aagggttatt ccgtcaggta gagcattcta tgtaccggtg
     4741 gcttcgaatg atacaaagga aggcaaaggc aaaaatcgta gaacagaaat tatactttca
     4801 cccaaactta ctgcgttgta taatctgatt aacaagtaaa actgtattga aaaatttatt
     4861 ttcggtagtt tttacactta tcgctaccac cgccttttcg caaggagtac ggtgcggtac
     4921 ggttttttcc ggtaagcttg atgaactatt cataagtcat gcacctgtaa gccggtcggt
     4981 tataaccatt ccggtagtat ttcatatagt atggcgtgat tcatccgata atatacctga
     5041 tgctcgtatc acttcacagc tcgatgtgct gaataccgat ttcagggcat tgaatgacca
     5101 gcttaaatac ctgtcttctg caaagaggag cctggctgct gatatggaaa tcgaattttg
     5161 ccttgccgct gttgatccgc agggcattcc tacaacaggc atcctcaggt ataagtctga
     5221 ggtggaaaac atcggtacac agaatacgcc taccatcccg tcaaaagcat ttatcaagca
     5281 taaaaatctc ggtggagcag atgcctggga cacacagcga tacctcaaca tctgggttgg
     5341 aaaatttcag gatggcgcta tacttgccga atcaaaattt ccttgggaca atctgcccga
     5401 agaagatggt ataagaatag atccggctta tgtaggtgtc aattgcaacg gttcgccgaa
     5461 gaaacagttt gccttgggga ggacattaac acacgaagtc ggccactacc tcggtctgct
     5521 acatccatgg agtgctgatt gtgctacagg cgactatgta gaagatacgc caccgcaacg
     5581 gtgcccgtat tatggttgta tgccacaggt gatggatgac gcttgtggat cggtgccttt
     5641 ggttgaaaat tttatgcagt tttcggatga tgcctgcctc gcactgttta cgcacggcca
     5701 gaaagaaaga gtgtatcaaa tgcttttgca gtaccgacct ttattgctga ataacaacat
     5761 ttcatgcttt aaaccaacat ttgccgacag gctcacagaa gacaatgtca ggatataccc
     5821 aaacccgaca agccattgta tatctattga ccttaggtta aatcgtgaga gtgatatcaa
     5881 ggtcgaaatc ttcgacacat cgggaaaact gatatggaaa gatgtcgtta aagtgctgtt
     5941 actcaggcca atagaattat catttacaca aagtggcatt tattttatac gccttacagg
     6001 caatgagaat actttggtac gtaaggtagt ggtgatgtga tgaaaaaaga cgtcttccct
     6061 aaaactgtgc atcctggaat cctttccggt atggaatatt caggaagtca agcgatgatg
     6121 gttttaccct gaggaaaaag gcataagtac ctcgttcggg ttgccagttc atacccattt
     6181 cccagcagtg caagtctcga tagaacccaa tgtcggggta ggtgagtcgt ttggctttga
     6241 agtcccaccc tatgttgccc acatctatcc tccagttttt ggttatcggg aggctaccgt
     6301 tgaggtcgag attgtgattt acaatagcga tggtgtcacc ttgttgaagc gggtaaatgc
     6361 caatgttgag cgtatgtctg atgaagatat tctgaagtaa ttcctgaggg acagagacgg
     6421 attgtggccc tcctgcattt tgtcggtctt cctgtatctc ccatatttgt ttgagagata
     6481 ttgcagagtt aaagctgaaa ttcgtattta caaagcgcag tagttttcct tttgatttgt
     6541 aataaagttt gttgatggtt cggccatagg catctacatc gtatgggtca aagctgccac
     6601 ttatggtgag tgtagtgatg cccttgaaga atctggcagt accgccatag cttaaacggc
     6661 tccagttgag tgaatcggca gcaaaattat agcttccgcc tatgtttata ttgtcgaaaa
     6721 acttaaattt tttgattgtt gaatcgcgtt tgtgataata ttttccttca aaaaggttat
     6781 tgataccata agcaaggtta agtgactgtt ttgagttggg tggcgttccg aatattccac
     6841 tctcgaatat agaatagcgc tgcatccggt tgaactctgg tctagagtcg gtattgatgg
     6901 tgccaaaata tcggtcgatg gaagcatagt cgggcgaata attaagcgat atgctgggtt
     6961 taatgacatg acgcacacca cgcagaaagc ctttcctgaa ctgaagcgaa ccgaatagtt
     7021 gagtgttggc actcaaactt gctgtgaagg tgcggaacgg ggcaaaacca tgtacagttt
     7081 tggatagaat cttaccgtaa tttgtcttgt aagtatagtc agttatttcg cccgcctggt
     7141 ttttaaccgg tatggtgtca atggtaattg tttcatcaaa accttttttt aaggtctgaa
     7201 aattccaggt ttcttcgtag cggatacttg gtgctacatt tacatacttg agtatccgtg
     7261 tattgtagct tgtactgatg ctttgtttga taccgtattt gctgttgcgg agtgtctccg
     7321 gtctgaaaag aagcgtatcg agtgttttaa tttcattttt tgcctgcatg tcgtaagtga
     7381 aatagatttt ttcgtaccat cgttcaggac ctatactttg cgctttgcgt ttaagaggat
     7441 aaatgctacc tgtctggtag ccaatatttg gaaaattgat aatgacctcg cgagagatat
     7501 tgttctgaga atgcgaaagt gatgccgtaa gtacctgact ggtgcccgga aatcttttgc
     7561 tgaaattgat attggacgaa agcgtattgt ttagtacact gttggcatcg ttgtagttta
     7621 ggctttgaaa attgttggac tgtatgttga cattgccacc aaatgttatg gagggatgtg
     7681 cttttgggtc ctgattgtgc tgccagatga aactgaatga tttgtcgctt gctattttgt
     7741 tggatcctgg tatttcgctt cttctgtcag aaaaagccat attgacgcca ccgttatatt
     7801 tgtatcgttg aacataccgt gtattggcac gaacacccca actaccacgg aagtaaatgt
     7861 cacccgttag ttttacatcg aagtgcttcc cgatgggaaa ataatagccg atgtctctaa
     7921 atccaatgcc ccatgtttca gaattttcgt aatctctagg caaaatgaga ccagctcgtt
     7981 gtcctttagt gattgggaaa aacccgaatg gcagtacaac aggcgttgga atgtggttaa
     8041 tttcgagaaa agaaggtccc actacagcta ccttgtctgc caccactttt tgtttcgaac
     8101 tcctgatgcc ataatgtggg tcaggatggt cgcaggtggt aatgagggca tttttgttat
     8161 agacaatggt ctgtttatgt aacgaatcct tcgctaccag gaatttagcc gtctcgccgt
     8221 gtacgaaaag gtcgttttcc ttactggtta tatctttagc aagccctttt ttcgatttga
     8281 aattgtattt tagttctttt gatttgaatt tttggtcacc atcagcgaaa tccgggaatc
     8341 cggtcatttt gccaagactg tctggtctgc ccatggcact cagtacgttg gtattccagt
     8401 caaatattat ataatttgcc tttaggtcaa tggttttgta cttcatccat gccttgttga
     8461 tgaggtggat gcgcttattg ttaatgtcat accagatagt gtcttttgcc gagcaatcca
     8521 cttctgcatc cagtgaatct tttgataatt tgaagtcggt atagcttaca agtactgaat
     8581 cacgtatggg cgtgccactg gtgtcagcag agacgatacc aagcgaatca ggccggagaa
     8641 caacgcctgg attctgttga gcaaccagca tacccggtaa tgcaaaagtt aaataaagga
     8701 aaaaattgta tattattctt gtattcaaac ccttttgata ttaactttgt ataaattaca
     8761 tttttttgta atacgaaata tattcccatg cctggcacaa cacaccttaa aacgacaaaa
     8821 catatattac gctgttactt ccttgttgtt tttaacattt tctttctgtt tcatttcctt
     8881 gaggctcagc caaagcaacc acaaaataat ggcaaagcta cacagttaca gaataataca
     8941 aaaaagaaaa atttaatccg taccattgtc attgatgccg gccatggtgg tcatgacccg
     9001 ggtactgtca attttaagaa aaaagaaaag gatatcacgc tcagtatcgc cctgaaagcc
     9061 ggtgagctca tagagcgcaa tttcaaagat gtacgtgtgg tgtacactcg caaaaaggat
     9121 gaattcattg acctgaaaaa acgtgcccag atagctaacc aggaacgtgc cgatttattt
     9181 atctccatcc attgcaacgc cattgaaacc aatcaggaaa atttttatgg tacagaaacc
     9241 tatgttcagg atccaaaagt agaaagttat aac
//
LOCUS       DAOQQY010000083         9240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2805_length_9240_cov_6.065433, whole genome shotgun sequence.
ACCESSION   DAOQQY010000083 DAOQQY010000000
VERSION     DAOQQY010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2805_length_9240_cov_6.065433"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1621
                     /locus_tag="PLE23_05600"
     CDS             116..1621
                     /locus_tag="PLE23_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82774.1"
                     /translation="MKKNIILPIFILVCTAISAHSQTVSDAVRYSYNTYANSGRSAGV
                     NNSLGAMGTDFGAISQNPGGLGWYRTSEFNFSLGTTGYTTNAQLSGTNNKSTKITARK
                     FGIPGVGLVFNSKPRHSDWKNVNFALGINQLGAYKQEYQYEGVSKGSVMDYFVEQAQG
                     KTADQLDPFSTKLAYEAFAIYDPDETNGNKTEWASDFDGFATKPVVAKKQEVLNTGYA
                     SEVVMALGANYKEKLSVGASLGVPFMRYETQSFYSEDNSGPDKVPFFNSLEWTEDVRA
                     KGVGVNLKIGASYRPIQALRIGAAIHSPTAYAISEDYNNSFVYDLTTANGTNYRTSKS
                     SPDGQYDYNLLTPWRWIGDAGIIIGKKGFISAEVEYVDYPTARFTFNNTSDDPDATKY
                     QRELNNKISKELEAGFNYRIGAEYVLGDFRVRAGYGIYATPYANDSQTNSSFSLGFGA
                     RLEKFFFDLGYVNWQQKQSFKPYEVSVKDYQPLVNSELSSSRILLTVGFKF"
     gene            1639..2661
                     /locus_tag="PLE23_05605"
     CDS             1639..2661
                     /locus_tag="PLE23_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82775.1"
                     /translation="MLRYHPAYRVFIYILLFFCVEIQESYGQGKNEFLYRDVPESILY
                     ETKWQYKYTLHVESQTIIHRAGDHFQAYYYFRLDKKAESVVNGTHSTGTWLLDRGKLN
                     APILNTDSLRVVKATDVELILEGSNKTGKGNLQYYFEHFGDDGQIFKKPEYLLPELTV
                     NTKRENVGKNSVAKWFKNFWRQLWGLEMDDSENLAGKPYINIEIIGGGYFGGIDPAEK
                     NYIQLKNNGRLIREYATQYQGQTKTIKDISRQELESLAEYLDTKGFFALQASYDCTDP
                     ECFKRKLKRPLPIPLRISVTYGLRHKVISVAMFGNDGVQDRYLSYPLLVDELVEILNR
                     MANRIE"
     gene            2963..4900
                     /locus_tag="PLE23_05610"
     CDS             2963..4900
                     /locus_tag="PLE23_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMX82776.1"
                     /translation="MKKEWPFLLCILLWANTMVAQDKLLIDSLYKQYNSEKDPKNRIE
                     LLYKIADEVEETGKPEEIVRYADSLELLAKAARYSKGLAKAYEIRGRYYERKGEHGQA
                     LPYYQKQLSLLVSVADKEGQARALTNIGNMWHELASNDSAVVYHIRAMRIMEELGKKS
                     DVAISLANIANAYSDLEAYDKAIEMLKQSLKIRREAGEEKRTMFTLNNLSVAYGRKGD
                     FENSMAYADTGIHVALKYNNKLVAGVICGGMGNILNGLKRYKESIAWCERSLGYLNEI
                     NRESNMVYPLSNMATAYIGLARYKEALYHNERGWAIMQKLNLAEPVEAYQQNFADAYA
                     GLGDYKQAFQWHKVYMARKDSLAGLDNLRKMASIEARYNIEKKERELLVQQTKNYRQK
                     VALYSLIAALAAFIVFAYMFYNRYRLRKRTELSEAVIHEQKLGMNAVIEAQETERKRI
                     ARDLHDGIAQEIVAIKLGFEILGRRIDKMAPEEMPKLSELSKQLDASCTELRGIAHNM
                     LPPTLGTQGLAPSLEMLLRNTLQHSNIKAKFNVHNLTTKIDEKTEISLYRIAQELLNN
                     IVKHSKAAQVVMELFQSDSWLVLRVEDDGQGFDFEAARSRGTMGILNILSRVGAVGGE
                     FKTEGNRTGGTIALVRVPLSI"
     gene            4935..5573
                     /locus_tag="PLE23_05615"
     CDS             4935..5573
                     /locus_tag="PLE23_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX82777.1"
                     /translation="MPSQIPILLVDDHHLVRKGFRALLEEIEGLKVVGEASNGKEAIM
                     LMRNEVKPAIVLLDYEMPVMNGLEAAEVINREFFGVKVIMLTMLEQKSLIEAAVEVGV
                     KGFLFKNTSLEDLGEAIMSVAKGGTWFGSDVALTLLRREYNPETEALAMLSNREIQIL
                     KLVAQGYTSMEISRKLFISPRTVDTHRNNIIQKLDLHGIPALVQFAMRTKLI"
     gene            5761..6255
                     /locus_tag="PLE23_05620"
     CDS             5761..6255
                     /locus_tag="PLE23_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMX82778.1"
                     /translation="MKNLIYSLLAVVFFSIQLHGQAPGKVWYGKGSGNAQAEAVHKAL
                     MSVVDDFANGNSNGWKIYTDDATEIDPNGNITFGKKAMLDAWDAFMKMVDEKPKFTYS
                     NVLVRMLTNDIAIAVFETSADIKIKGQQIGGKAKGSAVLRKHNGVWKIEFDQLTPEMP
                     MPEN"
     gene            6421..7416
                     /locus_tag="PLE23_05625"
     CDS             6421..7416
                     /locus_tag="PLE23_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMX82779.1"
                     /translation="MKRLLNILKWTGIVLISLFLVFFLFVQFSWDKKHGAPYPDIKAS
                     TDSAVIARGAYLANGPAHCGGCHTSMEDVMQFDEGKKVDFKGGWELTFPGFGTFRSHN
                     LSSDKETGIGSYTDAEIARSLRYGVGKDGRSLFPMMPFQGMSDEDLTAIISYLRTLPP
                     VKNQVKPMDYGFLYKALLAVGLVKPEGPKATPAKSVVVEPTAEYGKYLAYHVSNCFKC
                     HTQINENTGEFIGKDFAGKAYFLPDEFTDGYSFMSPNLTPDKETGIMASWSEDDFIKR
                     FKSGRVHKGSPMPWGSFSRMDTVELKALYRFLHSLEPAHNKIDKTVFAPGEKPKK"
     gene            complement(7595..7828)
                     /locus_tag="PLE23_05630"
     CDS             complement(7595..7828)
                     /locus_tag="PLE23_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82780.1"
                     /translation="MIAVSIRACPSAVAPGRSLPGFAIAPWQNATKALQAAHASIAVH
                     QEIYLITTEDIKSKYNQGMLQRLSVLPLQLKEL"
     gene            8125..9006
                     /locus_tag="PLE23_05635"
     CDS             8125..9006
                     /locus_tag="PLE23_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX82781.1"
                     /translation="MKYILQFLLGSVIILCVGVGCNKEDDVVQPAVYDVSKSYSLKDV
                     EYGKDAAQRMDVYLPANRSSDKTKVFVMIHGGGWSAGDKADYTATMDAFKNYFPGHAI
                     ININYRLGTQQSPGYPKQIQDIQSALDEIKKQKYNVSEQYLLFGGSAGAHLSLLYAYA
                     FDPDHHVKGVVNTVGPVDLTDTLLTGNVVLMGVVGGLLGKAIYDYKSNEALFVEASPA
                     KRVTATSPPTISFYGDKDPLIPVTQMPLLRKALEEKAVWHYDTIYPGGGHGLWASEAQ
                     NQDYINRTIGFINQFFK"
BASE COUNT         2841 a         1708 c         2224 g         2467 t
ORIGIN      
        1 attccaccca ttttgttaaa cagaagccaa ttgaagtatt ctgagagttg atttatttat
       61 taaatttgtc cgaagagagc aagtgatatt gcgggcaata caaaatttta ttacaatgaa
      121 aaaaaacatc atacttccga tttttatcct tgtttgtaca gccatcagtg cgcattcgca
      181 gactgtgtca gatgcagttc gttattcata caacacttac gccaattcgg gtcggagtgc
      241 cggcgtcaac aatagcttag gagccatggg tacagatttt ggcgccatca gtcagaatcc
      301 gggcggactg ggatggtatc gtacttccga gttcaatttt tcacttggta ccactggtta
      361 taccaccaat gcacaactct ccggcaccaa caacaaaagc acaaagataa cggccagaaa
      421 attcggaata cccggtgtgg ggctggtatt taactcaaag ccacgccact ctgactggaa
      481 aaatgtaaat tttgcattag gaatcaacca gcttggtgct tacaagcagg aatatcagta
      541 cgaaggtgta tcaaaaggat cggtgatgga ctattttgtc gagcaggctc aaggcaaaac
      601 agccgatcag ttggaccctt tcagtactaa attggcgtat gaagcatttg ctatctatga
      661 tccggatgaa accaacggta ataaaaccga gtgggcaagt gactttgatg gattcgctac
      721 gaaaccggta gtggcaaaaa aacaagaagt actcaacaca ggctatgcaa gtgaagtagt
      781 gatggcactc ggagctaatt ataaagagaa attgtctgta ggagcatcac ttggtgtgcc
      841 gtttatgcgg tatgaaaccc aaagttttta tagtgaagat aactcaggcc cggataaagt
      901 acctttcttt aattcgcttg aatggacaga ggatgtaagg gcaaagggtg taggcgtcaa
      961 ccttaagata ggtgccagtt acagaccgat tcaggcattg aggataggtg ctgccataca
     1021 ctcgccaaca gcctatgcga tatctgagga ttataataat tcgttcgtct atgacctgac
     1081 aactgctaat ggtacgaact accgaacaag caaatcgtca ccggatggcc agtacgatta
     1141 caatctgcta acaccgtggc gctggattgg tgatgccggt attattattg gcaagaaagg
     1201 ttttatttcg gccgaagtag aatatgttga ttatccgaca gccagattta cttttaataa
     1261 tacttcagac gacccggatg ctaccaagta ccaaagagaa ctcaataata aaataagcaa
     1321 agagctcgag gctggcttca actaccgtat cggtgcagaa tatgtgttgg gtgacttcag
     1381 ggtgagggca ggttatggta tttatgctac gccttatgcc aatgattctc agaccaattc
     1441 atcttttagc ctgggttttg gtgcccgatt ggagaagttt ttcttcgacc tgggttatgt
     1501 aaactggcag cagaaacaat cattcaaacc atacgaggta agtgtcaagg attatcagcc
     1561 tttagttaac tcagagttga gttctagcag gatactgctt actgttggat ttaaatttta
     1621 agaagatgca atgataatat gctaagatat catccggcat atcgggtatt catttatata
     1681 ctgttgtttt tttgtgttga aatacaggaa agctacgggc agggaaaaaa tgaattttta
     1741 taccgcgatg tgccggaaag catattgtac gaaaccaaat ggcaatacaa gtacacactt
     1801 catgtagagt cacagaccat cattcaccgg gcaggtgacc attttcaggc atattactat
     1861 ttcagacttg ataaaaaggc agaatctgta gtcaatggta cacattctac aggcacctgg
     1921 ctattggacc gaggcaagct taatgctccg atactcaata ccgattcgct tcgggtggta
     1981 aaagcaacag atgtggagct gatactggaa gggagcaaca aaacgggcaa gggcaatctg
     2041 caatattatt ttgaacattt cggtgatgat ggacaaatat tcaagaagcc tgaatatcta
     2101 ttgcctgagt tgacagttaa tacgaagcgc gaaaatgtgg gaaaaaattc tgtagccaaa
     2161 tggttcaaga atttctggcg gcagttgtgg ggactggaaa tggatgattc ggagaatttg
     2221 gcaggtaagc catacatcaa catagagatt atcggtggcg gatattttgg tggcatcgat
     2281 ccggcagaaa agaattatat tcagctaaag aacaatggca ggctcataag agaatatgca
     2341 acccaatatc agggacagac caaaactatc aaagacataa gccggcagga actcgagtca
     2401 ctggcagagt accttgatac aaagggattt tttgcacttc aggccagtta tgactgtaca
     2461 gaccctgaat gtttcaaacg aaagctcaaa cgaccattgc cgataccatt gcgtatatca
     2521 gtcacctacg ggcttaggca caaggtaatc agtgtggcca tgtttggcaa tgacggagtg
     2581 caggacagat acttgagcta tcctctgttg gttgatgaac ttgttgaaat actcaacagg
     2641 atggccaacc gtattgagta gcgcataaag gataaccatt tatgtcatca tttgggcaaa
     2701 taaatgaagc cggtacttat caatatcggt ttattttacc tctttatggt aatagttgga
     2761 cagaataggt atatgcagaa taattgttag gtctctatca tctctgtcag cataattttt
     2821 tttcctaagt ggcagaagta ttaaatcaaa ataaataact tcattcagaa aagatgtatt
     2881 ttgtgggctt aaatcacttt cagaagtttg gtaaaagtgg tgaatagcca ataaaatagt
     2941 aaagcaggca tcaaattgtg tcatgaaaaa ggaatggcca ttcttattgt gcatattatt
     3001 gtgggctaat actatggtcg cacaagacaa attattaatt gacagcttgt acaaacaata
     3061 caattctgaa aaagatccga aaaatcgtat tgaactctta tataagatcg cagatgaggt
     3121 agaagaaacc ggcaaacctg aagaaattgt acggtatgca gacagtcttg aattgcttgc
     3181 taaagctgcc aggtactcca aagggttggc aaaagcgtat gaaatacgcg gacgttatta
     3241 tgagaggaag ggcgaacatg gccaggcact gccttactac caaaaacaac tcagccttct
     3301 ggtctctgtg gctgataaag aaggccaggc cagggcattg acaaatattg gcaatatgtg
     3361 gcacgaactt gcatccaatg attctgctgt ggtgtaccat ataagggcaa tgagaataat
     3421 ggaagaattg ggcaaaaagt cggatgtggc catcagtctt gcaaatatag ccaatgccta
     3481 cagcgatctg gaagcctacg ataaggcaat agaaatgtta aagcaatcgc ttaaaataag
     3541 gcgggaggcc ggtgaggaga aacgcaccat gtttacgcta aataacctgt cggtagcgta
     3601 tggccgtaaa ggcgattttg aaaattcgat ggcgtatgct gatacaggaa tccatgtagc
     3661 gctcaaatac aacaataagc tggtagcggg tgtcatttgt ggtggaatgg gcaacattct
     3721 gaacggacta aaaagataca aagaaagcat tgcatggtgc gaacgttcgc tcggatatct
     3781 gaatgaaata aaccgcgaat ccaatatggt ttatccgttg agtaatatgg caacagctta
     3841 cataggtctg gctcgttata aagaggcact gtatcataat gaaagaggtt gggctattat
     3901 gcagaagctc aatcttgctg agcccgtaga agcatatcag caaaactttg cagacgcata
     3961 cgccggactt ggagactaca aacaagcatt tcaatggcat aaagtctata tggcccgaaa
     4021 agattcactg gcagggcttg ataacttaag aaaaatggcg agtattgaag cccgatataa
     4081 tatagaaaaa aaggaacgcg aattgcttgt acaacaaacc aaaaattacc ggcagaaagt
     4141 ggcgctttat tctctcatag cagcactggc agcgttcatt gtctttgctt atatgtttta
     4201 taacaggtac cgtttgcgta aaagaacaga actgtctgaa gccgtcatcc atgagcaaaa
     4261 acttgggatg aatgctgtta tagaagcaca agaaactgaa cgcaagcgta tagctcgtga
     4321 tctgcatgat ggcatagcac aagaaatcgt ggctattaag ctaggctttg aaatcttagg
     4381 gaggcgcata gacaagatgg cacctgaaga aatgcccaaa ctgtcagaac ttagcaaaca
     4441 actggatgca tcctgtacag agctacgagg tatagcccat aatatgctcc cgcctacact
     4501 cggtacacaa gggctggcgc ccagcctcga gatgttgttg aggaatacac tccagcattc
     4561 aaatataaaa gcaaaattta atgtccacaa cctgactaca aaaatagacg agaagacaga
     4621 gataagcctt tatcgtatag cccaggagtt gctgaataac atcgttaaac actcaaaggc
     4681 ggctcaagta gtcatggagc tttttcagtc agatagttgg cttgttctac gggtggaaga
     4741 tgatggtcag ggatttgatt ttgaagctgc acgttcgcgc ggtactatgg gcatcctgaa
     4801 tatcctgagt cgggtgggtg ctgtgggtgg agagtttaag actgaaggca ataggacagg
     4861 aggtacgatt gccttagtgc gagtaccatt gagtatttaa ccaacaacat attgaataag
     4921 caaatttaga aattatgcca agtcagatcc caatactttt agttgacgac caccacctgg
     4981 tacgtaaagg ttttcgtgcc ttgcttgaag agattgaagg cctcaaagtg gtaggtgaag
     5041 cttctaacgg aaaggaagca atcatgctga tgcgcaatga agtaaaacca gcgattgtat
     5101 tactcgatta tgagatgccg gtgatgaatg gacttgaagc agcggaagtc atcaatcgag
     5161 agttctttgg ggtaaaggtc atcatgctta ccatgcttga acagaaatcc cttatagagg
     5221 cagctgttga agtaggtgtg aagggttttt tgtttaaaaa tacttcattg gaagacctgg
     5281 gtgaagctat aatgagcgta gcaaaaggtg gaacttggtt tgggagtgat gtggcactta
     5341 cccttctcag gcgggaatat aatcctgaaa cagaagccct tgcaatgctt tcgaacagag
     5401 aaatacaaat cctcaaatta gtagcacaag gatatacgag tatggaaatc agtcgcaagc
     5461 tattcatcag tccaaggaca gtggatacac atcgaaataa tatcattcaa aagttagatt
     5521 tgcatggtat tccggctctg gttcagtttg ccatgcgtac caaactaatt tgataactcg
     5581 agcttgtaat atggtctatt tttttaacac tttacgtcct gtaatgtctc aaaagcgtat
     5641 tttacgtaaa tctgcgtagt gtatttgcgt acaattgctg attatggttt actatgattg
     5701 tctcattttt gtattgttca aaagatacat acatttttaa tcattaaacc ctaaatcaaa
     5761 atgaaaaatt taatttattc ccttttggct gttgtgtttt tttcgattca actgcatggt
     5821 caggcgcccg gaaaagtatg gtatggcaaa ggctcaggta atgcgcaggc tgaggcagta
     5881 cataaggcac ttatgtctgt agtagatgat tttgcaaatg gcaacagcaa tggatggaag
     5941 atttacacag atgacgctac agagattgac ccaaacggaa acattacttt tggaaagaag
     6001 gccatgctgg atgcctggga tgcattcatg aaaatggtag atgaaaaacc aaagtttaca
     6061 tatagcaatg tgctggttcg tatgcttaca aacgatattg caattgctgt ttttgagact
     6121 tctgccgaca taaaaataaa agggcagcaa ataggaggaa aagctaaagg atcggctgta
     6181 ctccgcaaac acaacggtgt atggaaaatc gagtttgacc agcttacgcc tgaaatgcct
     6241 atgcctgaaa attgattacc tgtatatatg tgcgacttgt aaagtaaagc caatatccgg
     6301 cttaacaatg cgtgcaatct gagatgatta gcagaaaaac aggcagagct tttcacccaa
     6361 tcatccactc ttaacccttg agtaagtatt attcttttca atgcataaaa tcaatgtcaa
     6421 atgaaacggt tacttaacat tttaaaatgg accggtatcg tactgatatc actgtttctg
     6481 gtattttttc tttttgttca attttcatgg gataaaaagc atggtgcacc ctatcctgac
     6541 atcaaggcaa gcactgattc cgctgtcata gcacggggtg cttatctggc aaacggtcct
     6601 gcacactgcg gcggatgcca cacctcaatg gaggatgtaa tgcagtttga tgaaggcaaa
     6661 aaggtggact tcaagggtgg atgggagctg acttttccag gattcggtac tttcagaagc
     6721 cataacctaa gctccgacaa ggagacagga ataggctctt atactgatgc cgagattgca
     6781 cgtagcctca ggtatggcgt aggtaaagat ggcaggtcac tttttccgat gatgcccttc
     6841 cagggaatga gtgacgaaga tcttactgcg atcatttctt atctacgtac cctgccaccc
     6901 gtaaagaatc aggtaaaacc aatggattac ggatttttat acaaggctct actggcagtg
     6961 ggattggtga agcccgaagg cccgaaagcc acgccagcca aatcagtggt tgtagaaccg
     7021 accgcagagt atggtaaata cctggcctat catgtaagca attgttttaa atgccacact
     7081 caaattaatg agaataccgg tgagtttatt ggtaaggatt ttgctggtaa ggcatatttt
     7141 ctacctgatg aatttactga cggatattca tttatgtcgc ccaacctgac acctgataaa
     7201 gaaaccggaa ttatggcttc gtggtcggag gatgatttta taaaacgctt caagagtggc
     7261 agggtacaca aaggaagccc aatgccatgg ggatctttct caagaatgga tacggtagag
     7321 cttaaggcat tgtacagatt ccttcattcg cttgagcctg cccataataa aatagacaag
     7381 acagtatttg cgccgggaga gaaaccaaag aaatgaagca caaacaaacg ggaacaattg
     7441 ggtataaaca atcgtttgag tgccttgggt tgttgttcaa tgcttcgttg ggagctcagt
     7501 ggtaaaaaat ctaaaccacc aaatagtttg aaaccgttct gcacaaggca aaaggctcag
     7561 cgagatcaac gaaagaccaa tgcactttga agatttaaag ttccttcaac tgaagcggta
     7621 agacactaag cctttgtagc ataccttgat tgtacttgga tttgatatct tctgttgtga
     7681 tcaaataaat ttcttggtgt acggctattg atgcgtgagc tgcctggagg gctttggtgg
     7741 cattttgcca cggagcgata gcgaaccccg gaagggagcg acccggagcg acagcggagg
     7801 ggcacgccct aatgctaact gcgatcatga atctttgttg taataactcg ctgcactgaa
     7861 atcaggcgga agcataccaa tgtggtgggc aaccggtctg gaaatatgct tatacacaag
     7921 aatctgatgt tgtgcaatgt aagatgtatg cacggtatta agaataatta tgatgaaatg
     7981 gatgatgcat ggacgtgcgg ctgtcggagt aatttttttt aagtgaattt tttgtgaaaa
     8041 gtatttaatt ttacaatatg gtagctgtgg aaagagttat tgaagggtaa ggaaattgat
     8101 ttgattgaaa aaatccaaaa aacgatgaaa tatattctcc agtttttact tggctcggtc
     8161 attatattgt gcgtaggtgt tgggtgcaac aaggaagatg acgtggtaca accggcggtg
     8221 tatgatgtgt cgaaatcata tagcctcaag gatgtggaat atggtaaaga cgcagcgcaa
     8281 aggatggatg tttatttgcc ggccaaccgg agtagcgata agacaaaggt gtttgtaatg
     8341 atacatggtg gcggttggag tgccggtgac aaggcggatt atacagctac aatggatgcg
     8401 tttaagaatt attttccggg tcatgctatc atcaacatta actaccggct cggtacgcag
     8461 cagagtccgg gttatccgaa gcagatacag gatatacagt cggcattgga tgagataaag
     8521 aagcagaaat ataatgtttc ggagcagtat ttgctctttg gcgggtcggc gggagctcat
     8581 ctttcgcttt tgtatgctta tgcttttgac cccgaccacc atgtaaaagg ggtagtcaat
     8641 acagtagggc cggttgattt gactgatact ttgctgacgg gaaatgtagt gctaatgggt
     8701 gtagtgggcg gattgttggg caaggccatc tatgattata agtcgaatga agcattgttt
     8761 gtagaggcga gtccggcaaa gcgggtgacg gcaacatcgc caccgaccat ttcattctat
     8821 ggggacaagg atccgctgat accggtgaca cagatgccgc tgttgcgcaa ggcacttgaa
     8881 gaaaaagctg tatggcacta cgataccatt tatccgggtg gtggtcatgg tctttgggcg
     8941 tcagaggcac aaaatcagga ttatataaac cgtacgattg ggttcattaa ccagtttttt
     9001 aaataggtgg ttgggcgtac tagttgaagg ccgcattctg agccggtgtg ccggaatacg
     9061 tttggctgcg ttaaattgta caagtgctgc atcctgtgtt gtgcctgtca ggatacttta
     9121 ttcagttcga ctgtaaaggt acttccgcta ccgagttcgc tattgacaga tatggtacca
     9181 ccttgagtgg acattctcat gagaatagac ataaagataa gctattatgc tgctaatttt
//
LOCUS       DAOQQY010000084         9239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2806_length_9239_cov_6.395579, whole genome shotgun sequence.
ACCESSION   DAOQQY010000084 DAOQQY010000000
VERSION     DAOQQY010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2806_length_9239_cov_6.395579"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="PLE23_05640"
     CDS             complement(<1..2287)
                     /locus_tag="PLE23_05640"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HMX82782.1"
                     /translation="MAVRTTEQPVIDGMLVESFWKNVPVATDFIQNQPEVGKPSAKKT
                     EVRVAYDDEAIYIGAMMYDDPALIRKQLTARDEEDFKDIDYFAVFFDTYHDKQNGFGF
                     LVSAANVQTDAKLSSGEDNNRDKSWDAVWTSEVKILADGWSVEMRIPYISLRFANKNI
                     QNWGIQFARQIRRFNENSYWNEVKPELNGFVDQFGIFMGITNLQPPLRLSFSPYLSTG
                     FRSSPDEGDSYHKTWLRSGGLDLKYGVNESFTLDATLIPDFGQVVSDNAVNNLTPYEV
                     QFEENRQFFTEGTELFNKAGLFYSRRIGARPSDYYTVREFAGENPQWKLLDNPSLTQL
                     YNAVKFSGRNKHNLGIGVFNGITAPMYARLRNDVTKRDTSILTAPLTNYNILVLDQAL
                     KGRSSVTFTNTNVMRNGYYQDANLSSLDFSLFDKTQLYNVRGRARYSKSFSSNNPNSG
                     FNTAIAIAKVGGSWQYFVQTNIESDQYDANDLGYLYAPNEVSVEGGISYWQYNPGKVF
                     QTYNFSFNSGYERLYKPTAYTDLWTEFSAFGIFRNYWDVNFKAGYNALKHDYFVLGNP
                     ATYRRFVQRPAFTYYELSGSTDSRRKLFYAYEFTLHSFIHPMVPKHHYELYSSVRYRF
                     NEHLSMDISYSFETESNYIISAGRESDGSPLVAFVDYTEESTILSGTYNFTPRLNLTL
                     RLRHYLSRVRHNTLAKIGEDGVPQPLSVLTESDNVNIFNADAFLTWDFRPGSRVVLGF
                     KNWLGPDESVDLQGKENNYFNN"
     gene            2791..3360
                     /locus_tag="PLE23_05645"
     CDS             2791..3360
                     /locus_tag="PLE23_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002664853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HMX82783.1"
                     /translation="MFELIKQPWHWAVSGILIGLTVPTLLILGNKKFGISSSFRHVCA
                     ACIPSRIPFFQYNWRKEIWNLFFVTGILVGGFIAANFLSNPAQFVIAESTKSALSELG
                     IRDFSNILPKDIFNSANIFTFKGLFFFIVGGFLVGFGTRYAGGCTSGHSITGLSNLQF
                     SSLVATICFMIGGFISANIILPFILHILN"
     gene            3362..3856
                     /locus_tag="PLE23_05650"
     CDS             3362..3856
                     /locus_tag="PLE23_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HMX82784.1"
                     /translation="MKETESSKAIRHQGTICTNESEKKEIGLAGNLKYLVAGIVFGII
                     LVKAEIISWFRIQEMFRLQSFFMYGVIGTAVVTGIISIWTIKRYNIKTWYGERIVFDK
                     KPYNKGQIYGGLIFGIGWAITGACPGPLFAQIGSGYVVAVITLLSAIAGTWVYGYLSH
                     KLPQ"
     gene            complement(3908..4300)
                     /locus_tag="PLE23_05655"
     CDS             complement(3908..4300)
                     /locus_tag="PLE23_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMX82785.1"
                     /translation="MKNLTEISILVMLAIIMLQSGVDKLTDRKGNLDWMTSHFAKSPL
                     KSMVPMLLSVVTIMELAAGVTAVLGAVTLFTSGNTTFAELSCLLSIANFMALLFGQRM
                     AKDYVGAQTIVIHLIPTIFLMYLLTRNI"
     gene            complement(4467..4799)
                     /locus_tag="PLE23_05660"
     CDS             complement(4467..4799)
                     /locus_tag="PLE23_05660"
                     /inference="COORDINATES: protein motif:HMM:NF039419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6370 family protein"
                     /protein_id="HMX82786.1"
                     /translation="MFFSAISYGQVAKGLAKKPDASKKVMTVEASCGECNFGLPGNNC
                     DLAVKIDGKAYFVDGVDISHYGHPHDKNGFCMVVRQAEVQGEVKDGRFKASYFKLLNV
                     EKAKQSGK"
     gene            4922..5119
                     /locus_tag="PLE23_05665"
     CDS             4922..5119
                     /locus_tag="PLE23_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82787.1"
                     /translation="MFRFCRNNLFILYDIYYSFSENGINTRPLSSASVTFGYIDMVSD
                     PETGYLYTGDGVYMMCLKPPK"
     gene            5345..6415
                     /locus_tag="PLE23_05670"
     CDS             5345..6415
                     /locus_tag="PLE23_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82788.1"
                     /translation="MIATITNTLIAQTPPDPVRCITYDVSTIVKYGIPATMSPDSLSA
                     FELAGIRPYVEDSKVLYTYFQSGKSTQTIIYPHGHNNVDAWAVPIYKSETIDNVVNLY
                     DIDNNLVFSEEMGLSYSLPPLPNDQIPNLGYYSPITVPSNNDIFMMVSEGYSYFTDNN
                     KHVFISDSTQFILDPVNQNSEIRILEDGKVVLTNFEQLIQTDNQKYLPSYRITKSYLQ
                     NLTGDNRIMKYEKKVFSNYNFSCEIDPQQSQTHSSLYQASTKNQNLSVFHYTPEANPK
                     ISTLNIGMFPNPAGNLINFVMPYPLDEDIEIYSGSGSFLSKHKLDSTQSVIDITHLPN
                     GQYIVRITTNHEVFIKKLIIQK"
     gene            6442..8043
                     /locus_tag="PLE23_05675"
     CDS             6442..8043
                     /locus_tag="PLE23_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82789.1"
                     /translation="MKNILSIIAVLFVCHNINSQIILPENPFDSFGNPSIRKPIEINR
                     NGTHHQDIDINEGNYPPYPPIDPETDGEKYVFWVHGLNGDIGSWIRAADASQNNVANG
                     FPARKLKSVSMIDYTQDANLYGAAQQLGEIIENKRSDQLALGEDPTQNFIIGHSQGGI
                     VTRALLHRDLCLNHTPVNNLGYGGIITFGSPNQGARILNNKHLFNDMSVDMCKSLGAG
                     PAEELSQKTVADLFIFKINVGKIIPVKDIVDNICTSFVGNLVDLAVMNQTPNITQSYY
                     VGAPDITNISNCSDQDLVSFPKVAFYGIEPPEGLLFRTGAYFLKSPNEFDYFQANDDY
                     FLTNAFIENKKKYDAAVAKWEYYYFLAQVERIQYCKLPNVLLDWPMCLSAKKREMQTL
                     KILNAYKQGQDWFNRVDDQYKTIIGAIDYTKTTQWYCECENVKTGQFTSTPVAGPGDC
                     PPSSKNGIVCSPVEYTIFSQIKKDSDGVVLSESAMNIPYATAPPRKMYNSSHMQMRND
                     DNTKKALLEIYDGDVGPAYKTAKKN"
     gene            8078..>9239
                     /locus_tag="PLE23_05680"
     CDS             8078..>9239
                     /locus_tag="PLE23_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HMX82790.1"
                     /translation="MNRKYINQIGILLMVGIGIVLLACKKKEMPAPNPCDNPPCNTTP
                     KDSFMVWQVPMTPDYKYGFSMDPVLDGNYLYFSNLFTAPGAPIHKVNKLTGEHIWITN
                     SDCKYEQETRNFFYQTESKIVLKQYYCLNFRNKSDGAILNEINFTDSDNGACDFRLTG
                     FGNWVFIITTQYNKDPLTARNNLVRCNIDSYKMDTLFSLPYLNGYEQAIEPPAAYVAP
                     GGDTMLLFQSRNYNFKKVDGRIDFFCFNMTQRKLVWRLEDIEENGTGSINTPQIVGDR
                     VYYQGSNELFMFDIATGNKIWSRRFPGESYFYTKNQIVGDRIFVKDDGDRFKALDLNT
                     GNTLWEAYGEYGGGLGPAYANGNVFVIGTDEGYAAIYGLRADTGKQYWKYRTP"
BASE COUNT         2869 a         1867 c         1781 g         2722 t
ORIGIN      
        1 ggttgttgaa ataattattt tcttttccct gcaaatcaac actttcgtcg ggaccgagcc
       61 agtttttaaa accgagtacc accctgcttc ccggccgaaa gtcccaagta agaaatgcat
      121 cggcattgaa aatgttgaca ttgtcggatt ctgtcaatac agataaaggc tgtggcactc
      181 catcctcacc aatctttgcc aatgtgttat gcctgactct gctgaggtaa tggcggaggc
      241 gtagtgtaag attgagcctt ggcgtaaaat tgtacgtgcc tgacaagata gtactttctt
      301 cggtatagtc aacaaaagct accagagggc taccatctga ttctctgccc gcactgataa
      361 tatagtttga ttctgtctca aaactgtaac tgatatccat actcaggtgt tcgttgaatc
      421 tgtagcgcac acttgaatat aattcgtaat gatgttttgg taccatagga tggataaatg
      481 agtgcaatgt aaattcgtat gcatagaaaa gctttcggcg gctgtctgtt gatccgctca
      541 gttcgtagta tgtaaatgca ggcctttgca caaatcttcg gtacgtagca ggattgccaa
      601 gtacaaaata atcgtgcttc agggcattat agcctgcttt gaagtttaca tcccagtaat
      661 tgcgaaatat accgaatgca ctaaactcag tccacagatc ggtatatgcg gtaggcttgt
      721 acagcctttc ataaccggag ttaaaactaa aattgtaggt ctggaatacc tttccaggat
      781 tgtattgcca ataggagatg ccaccttcaa ccgaaacttc attaggggca tacaagtatc
      841 caagatcgtt ggcgtcatat tgatccgatt ctatatttgt ctgtacgaaa tactgccaac
      901 tgcctccaac cttagcgatg gcaattgctg tattgaaacc cgagttcgga ttattacttg
      961 aaaaagattt gctgtacctt gctcttcccc ttacattgta aagctgtgtt ttatcaaaaa
     1021 ggctaaaatc cagagaactc aggttggcat cttggtaata tccgttgcgc attacattgg
     1081 tattggtgaa tgtaacagac gaccgacctt tcaatgcctg atctagtaca aggatgttgt
     1141 agttcgttaa tggagcagta aggatgcttg tatctcgctt agtgacatca ttcctgagcc
     1201 gggcgtacat aggagcagta atgccattaa acacgcctat acccaggttg tgtttgtttc
     1261 tgccggaaaa tttgactgcg ttataaagct gagtaaggga aggattgtca agcagtttcc
     1321 actgtggatt ttctccggca aattccctga cagtataata atcggatggt cgggcgccta
     1381 tacgacgcga ataaaacaaa ccggctttgt tgaataattc ggtgccttct gtaaagaact
     1441 gtctgttttc ttcgaattgc acttcgtaag gtgtcaggtt attgacagca ttgtctgata
     1501 ctacctgtcc gaaatcgggg atgagtgtgg catcgagcgt aaaactttca ttgacaccat
     1561 acttgaggtc gagcccacca ctgcgaagcc atgttttgtg gtacgaatca ccttcatcgg
     1621 gcgagcttct gaatccggtg gacagatacg gcgaaaaact aagtcgtaga ggtggctgaa
     1681 gattggtgat gcccataaag atgccaaact gatcgacaaa tccgttgagc tccggcttta
     1741 cttcgttcca gtagctgttt tcattaaaac gccttatctg ccgggcaaac tgaatgcccc
     1801 agttctgtat atttttgtta gcaaacctaa gtgatatata aggaatgcgc atttctaccg
     1861 accatccgtc ggccaatatt tttacttcac tcgtccacac agcgtcccac gatttgtctc
     1921 ggttgttgtc ttctcctgat gaaagcttag catctgtctg aacattagcc gcactgacaa
     1981 ggaatccgaa accgttttgc ttgtcgtgat aagtatcaaa aaacaccgca aagtaatcga
     2041 tgtccttaaa gtcttcttca tcgcgagctg tcagctgctt ccttataagg gcagggtcgt
     2101 cgtacatcat ggctcctata taaatggcct catcgtcata ggccacacgt acttcagtct
     2161 tttttgcgga tggtttgcct acttcgggct ggttttggat aaaatcagta gctacgggta
     2221 cattcttcca aaagctttcc accagcatgc cgtctatcac cggttgttcg gttgttctga
     2281 cagccacaag cgtttttggc tgagaaaaga ctgtatttgc gaataatagc aaaacaaaga
     2341 gattcactat tttattcgga aaaaatttaa aacaaaatat tttcattaac tgtttgaaga
     2401 ttgtacgcaa atgaacattg gtattaaaaa acggccaaat ataatgtaca tttaaagtaa
     2461 attgtataaa atctgtttga taatgatgga aacaacacta atatttattt ggcagatttt
     2521 ttgagtattt ttatttacaa aggaacattt atcattctaa agaaattact tgtaaaacaa
     2581 tatcactaaa atacagtttc taatgacaac aatgccataa tactgcaaaa ggctcacgga
     2641 aagtagttat ttacctgatt taaaaaatta aaaattgcct tcacatagtg tttattcaac
     2701 agttttaggt aaattttcat atcaaatacc taacatgctt attttgtaaa aaacaataaa
     2761 ttgaatgatt aaccaacaat attctgactt atgtttgaat taataaaaca accttggcac
     2821 tgggcagttt caggcatttt aatcgggctt actgtaccta ccttgcttat tctgggcaat
     2881 aaaaaatttg gaatttcatc gtcattcagg catgtatgtg cagcctgtat tccttcaaga
     2941 attccatttt ttcaatacaa ttggcggaaa gaaatatgga atctgttttt tgtaaccgga
     3001 atactggtcg gcggattcat tgctgccaat tttctgagta accctgctca atttgtgata
     3061 gcagaaagca caaagtcggc attgagcgaa cttggcatcc gagatttttc taatatttta
     3121 ccaaaagaca ttttcaactc tgcgaatatt tttacattca aaggtctttt cttctttatt
     3181 gtcggaggat tcttggttgg ctttggtacc agatatgcag gtggctgcac cagcggacat
     3241 tctattaccg ggctgtcaaa tctgcaattt tcatcattag tagctaccat ttgttttatg
     3301 atcggaggct ttatttcggc caatatcatc cttccattca tcctccacat acttaactga
     3361 catgaaagaa acagaatcaa gcaaagcaat ccgacatcag ggtaccatct gtaccaacga
     3421 aagcgagaaa aaggaaattg ggctagccgg caatctgaaa tatcttgttg ccggtatcgt
     3481 attcggcatc attttggtaa aagccgagat catcagctgg ttccgtattc aggaaatgtt
     3541 caggcttcaa tcatttttta tgtatggcgt tattggtaca gcggttgtca ccggtatcat
     3601 ctcgatttgg accatcaagc ggtataacat aaagacctgg tatggcgaaa gaatcgtatt
     3661 tgacaaaaag ccatacaata aaggccagat ctatggtgga cttatatttg gtatagggtg
     3721 ggctattacc ggagcttgtc cgggtcccct ttttgcgcag attggcagcg gatatgttgt
     3781 cgcggtcatc acgctgttga gtgccatagc cggcacttgg gtgtatggat atttgagcca
     3841 caagttaccc caataggtca atattgcaat accgcaaatg ttaatcaatc ttatttggca
     3901 aggaaaatca aatattcctt gtaagcaaat acatcaaaaa aatggtcggt ataaggtgaa
     3961 tgacgatggt ctgcgcaccc acgtaatcct ttgccatccg ctgtccgaag agcagtgcca
     4021 taaaattggc aattgaaagt aggcaactca gctctgcaaa tgtagtattg ccacttgtaa
     4081 acaacgtaac tgcacctaac actgctgtca cacctgctgc cagctccata atagtgacta
     4141 cagacagcaa catcggcacc atgctcttaa gtggcgattt agcaaagtga cttgtcatcc
     4201 agtcgaggtt tccttttcgg tcggtcaatt tgtccacacc ggattgtaac atgatgatag
     4261 ccaacattac aagtatggaa atttcggtaa gattcttcat tttttgtctg tttggtttgt
     4321 gtaacgaata ttttccagac aaaattagtg tcaggttttt aaatttcaaa caagaaatac
     4381 aagactattt tcactccaaa taaccagaag tgaacaatca tcaatccttc gcttgccttg
     4441 tatcctggcg tgaacagaaa atcaacttat ttgccagact gtttagcttt ttctacattc
     4501 aggagtttaa aataactggc tttgaaccga ccatccttta cttcaccttg aacttcagct
     4561 tgtcttacaa ccatacaaaa tccgtttttg tcatgcggat gaccataatg agatatgtca
     4621 acaccatcga caaaatatgc cttaccgtca atctttacgg ccaggtcgca gttattaccc
     4681 ggaaggccga aattgcactc gccgcatgaa gcttccacgg tcatgacttt tttggaggca
     4741 tccggttttt tagcaagacc cttggctacc tgtccatagc tgattgctga aaaaaacata
     4801 aaggccacaa ataaataaac tgattttaca atagattttt taaacatgat attagcaata
     4861 ttttgatctt acaatataaa gtaaccataa acttctattt gtaaacatac aaaaacagca
     4921 tttgtttaga ttctgccgaa acaatttatt tattctctat gatatttact acagcttcag
     4981 tgaaaatggg ataaacactc gcccattgag cagcgcatca gtcaccttcg gctacataga
     5041 catggtcagc gaccccgaaa ccggctatct ctacaccggc gacggcgtgt atatgatgtg
     5101 tctgaagccg ccgaaatgat gagcagataa aatgaaagga cttgatacca gaaaatcacg
     5161 atcaaaatat ttaatatatt attaaatact ttaccatatt ttattttgtt atttaataat
     5221 tattttattt tttttcattt ttacttgcat atacaaaagc atacatccat ttttgtaaca
     5281 ttaactaaca aaacagttga aatcatgaaa aaagtactta ccccccccgc ttgcaattat
     5341 attgttgatt gccactatta caaatacact cattgcccaa actccaccgg accctgtgag
     5401 gtgcatcact tatgatgtca gtacaattgt gaaatatggt attcctgcaa ccatgagtcc
     5461 tgattctcta tccgcttttg aattggctgg tattcggcct tatgttgagg attcaaaggt
     5521 tttgtacact tatttccaaa gtggaaaatc aacacagact atcatttacc cacatggtca
     5581 caacaatgtt gatgcctggg cagtaccgat ttataaatca gaaaccattg ataatgtagt
     5641 taatttatac gatattgaca acaatctggt tttttcagaa gaaatgggct tgagttattc
     5701 attgcctcct ttaccaaatg accaaatacc aaatttggga tactatagcc ctatcacagt
     5761 tccatctaat aatgatatct ttatgatggt ttctgagggg tatagctatt tcacagataa
     5821 taacaaacat gtttttattt ccgattcaac ccagtttata ttagaccctg taaaccaaaa
     5881 ctcggaaata cggatattgg aggacggaaa ggttgtttta acaaactttg aacagctaat
     5941 acaaactgac aatcaaaaat atttaccttc atatcgaatc accaaaagct atcttcaaaa
     6001 tctgacagga gacaaccgga taatgaagta cgaaaaaaaa gtattctcaa actataattt
     6061 tagttgtgaa attgaccccc agcaatctca gacacattca tcattgtatc aagcctcaac
     6121 aaagaaccag aacctttcag tattccatta cactccggaa gcgaacccta agatctctac
     6181 attaaatatc ggtatgtttc caaaccccgc cggtaatttg ataaattttg tcatgcctta
     6241 tcctcttgat gaggatattg aaatttattc tggttcaggt agttttttaa gtaaacataa
     6301 gcttgattcc acacaatccg tcattgatat tacacatttg cctaatggac aatatatagt
     6361 gcggattacg actaaccatg aagtttttat taaaaaacta atcattcaaa aataattgac
     6421 tgacaataaa aatatatagt catgaaaaat atattatcaa taatagccgt attgtttgta
     6481 tgtcacaata tcaattcaca gataatttta ccggaaaatc catttgatag cttcggaaat
     6541 ccatccattc gcaaacccat tgaaataaat agaaacggga cacatcatca ggatatagac
     6601 ataaacgaag gaaactatcc tccgtaccct cctatcgatc ctgaaaccga tggagaaaaa
     6661 tatgtctttt gggtacacgg acttaacgga gacatcggtt cttggatacg ggccgctgac
     6721 gcctctcaaa ataatgtagc taacggtttt ccagccagaa agctcaaatc tgtatccatg
     6781 atagattata cccaagatgc caacttgtat ggtgctgcac aacaattggg tgaaataatt
     6841 gagaataaaa gatcagatca attagcattg ggtgaagatc ctacccaaaa ctttattatc
     6901 ggtcacagtc aaggcggcat agtcaccaga gcgttattac acagagattt atgtctgaat
     6961 cacacacccg tcaataactt aggatatggt ggtattatca cattcggctc acccaaccaa
     7021 ggagcaagaa tactgaataa taaacacttg tttaacgaca tgtcagtgga catgtgtaaa
     7081 tcgctggggg caggaccagc tgaagaactc tcccaaaaaa ctgtggcaga tttgtttatt
     7141 tttaaaataa atgtaggtaa aattattcct gtaaaagata ttgttgacaa tatttgcaca
     7201 tcctttgttg ggaatcttgt agatttggca gtaatgaatc agactccaaa tatcacacaa
     7261 agctattacg taggtgcgcc tgacataaca aacatcagta attgctcaga tcaagatttg
     7321 gtttcatttc cgaaggtagc attttatggc atagaaccgc ctgaaggctt attgttccgt
     7381 acaggtgcat actttctgaa aagcccgaat gagtttgatt attttcaagc aaatgacgac
     7441 tatttcctaa caaatgcatt tatcgaaaac aaaaaaaaat atgatgctgc agttgccaaa
     7501 tgggaatatt attattttct tgcccaggtg gaacggattc aatactgcaa gttgcctaat
     7561 gttttgctcg attggccaat gtgcttatcg gcaaaaaaga gagagatgca aaccttgaaa
     7621 atattgaatg cttataaaca aggacaggac tggtttaaca gagttgatga ccagtacaaa
     7681 acaatcatag gggctattga ttacacaaaa actacacaat ggtattgcga atgtgaaaat
     7741 gtaaaaaccg gacaattcac ttctactcct gtagctggcc cgggcgattg ccctccaagc
     7801 tccaaaaatg gtatagtttg ttcaccggta gaatatacta tcttttctca aataaaaaaa
     7861 gatagtgacg gcgttgtcct ctctgagtct gccatgaaca tcccttatgc aactgcacca
     7921 ccccgcaaaa tgtataattc ttcgcacatg cagatgagaa atgatgataa tacgaaaaaa
     7981 gcactattgg aaatttatga cggagatgta ggtcctgcat ataaaacagc aaagaagaac
     8041 taatgaatca ttttattcaa ttttaaacag agattaaatg aacagaaaat atataaatca
     8101 aattggtatt ctcttgatgg tgggcatagg aatcgtcttg ttagcctgca agaagaaaga
     8161 aatgcccgca cccaaccctt gcgacaatcc gccctgcaat accacaccaa aagattcatt
     8221 catggtgtgg caggtaccca tgacaccaga ttataagtac gggttttcta tggatccggt
     8281 attggatgga aattacctgt atttctccaa tttgtttact gccccgggtg ctccaataca
     8341 caaggtcaat aaattgacag gagagcatat ttggatcacc aattcagatt gtaaatatga
     8401 acaggaaact agaaatttct tttatcaaac tgaatcaaaa attgtattaa agcaatatta
     8461 ctgcctcaac ttccgcaata aaagtgacgg tgcaatattg aatgaaatca acttcacgga
     8521 ttctgacaat ggggcttgcg attttcggtt gaccggattt ggcaattggg tttttattat
     8581 aacaacccag tacaataaag acccgctcac agccagaaac aatctggtga ggtgcaacat
     8641 cgacagctac aaaatggata ctttgttctc cctaccatat ttaaatggtt acgaacaagc
     8701 tattgaacct ccagctgctt atgtagctcc tggtggcgac accatgctct tgtttcagag
     8761 ccggaattat aattttaaaa aagttgacgg ccggatagat tttttctgct tcaacatgac
     8821 ccaaagaaaa ctcgtatggc gccttgagga catcgaagag aacggcacag gttctatcaa
     8881 tactccacag atagtgggcg acagggtcta ttatcagggc tcaaatgagc tatttatgtt
     8941 tgatattgct accggcaaca agatctggag caggcgcttc ccgggtgaaa gctactttta
     9001 cacaaaaaac cagatagtag gcgacagaat tttcgttaag gacgatggcg acagattcaa
     9061 ggcactcgat ctgaacacag gcaataccct gtgggaagcc tatggagaat atggcggtgg
     9121 actcggccct gcgtacgcca atggtaatgt ttttgttata ggtaccgatg aaggttatgc
     9181 tgccatctat ggattgcgtg ccgacacggg caagcagtac tggaaataca gaactccca
//
LOCUS       DAOQQY010000085         9220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2820_length_9220_cov_5.645172, whole genome shotgun sequence.
ACCESSION   DAOQQY010000085 DAOQQY010000000
VERSION     DAOQQY010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2820_length_9220_cov_5.645172"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="PLE23_05685"
     CDS             complement(<1..131)
                     /locus_tag="PLE23_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HMX82791.1"
                     /translation="MGNRNIVLAGNPNCGKTTIFNLLTGLNQKTGNFPGVTVDKKHAV
                     "
     gene            complement(141..383)
                     /locus_tag="PLE23_05690"
     CDS             complement(141..383)
                     /locus_tag="PLE23_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HMX82792.1"
                     /translation="MNLNLGNTVKLSELQAGEMGIIDELVVHNFTTRLLAMGIIPGKK
                     IKLLRRQPWQGSLYIDVDNHYIGLRLQEAELILIKK"
     gene            complement(442..1119)
                     /gene="cmk"
                     /locus_tag="PLE23_05695"
     CDS             complement(442..1119)
                     /gene="cmk"
                     /locus_tag="PLE23_05695"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HMX82793.1"
                     /translation="MKKIIIAIDGYSSCGKSTLARELARVLHYIYIDTGAMYRAVTLY
                     FINHNIDFKNEEEVTNALNNISIDFRKIKSEIHTFLNSQDVETSIRSMQVSELVSEVS
                     AISSVRSFLVQQQQAMEQQKGIVMDGRDIGTVVFPSAELKIFLTASPEIRATRRKKEY
                     EQKGIDISLEEVKNNLAHRDHIDSTREDSPLRQAADAILLDNSYLTKEEQLKFVLDLM
                     SEKFNLH"
     gene            complement(1151..2659)
                     /locus_tag="PLE23_05700"
     CDS             complement(1151..2659)
                     /locus_tag="PLE23_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82794.1"
                     /translation="MKHLLEISNIVSWKKVKKIEIFDEASLKNKSSKLNEFFEALMDG
                     KFKTDRDAAALLYQSSPTDDKYRQLKSRFRKRLLNTLFFIDINLPSASNYTRAYFSCN
                     KDWTLAKILLSYGAELTAHDIAGQILTIALKFKFADIIVNSARILRQNAAHNRDEKEF
                     QYYNQIINEYQDIILAEMRSEEINQFVRLRYENTSTHSESKNNELDDQCDQLILLSEK
                     YDSPVITFNMFYAWLVKFEYENDFHAVIEICIKMEDYITHNPQYEQDDMLLTFQIKKL
                     NAFLNLRDFENAKKDVEKFVKVFEEGSAHWHKYMDLYFLTAMHSDNFANALAIHSKVT
                     SSPKFRKAHRMLKEKWKIYEYYLYFMIENTQNNLQGIYKQKSTQLFESLMADPLIFAK
                     EYRSYTVQSVIAKTLVAYSNKNFLISAENVERLKSYTTRPTRPEENLRVNLFIKLLVQ
                     LVKSNFNTRFLSGYEKHLDNLANTPMKEHGQDFDLEIYPYEKLWKLLVSKLN"
     gene            2894..3394
                     /gene="lysM"
                     /locus_tag="PLE23_05705"
     CDS             2894..3394
                     /gene="lysM"
                     /locus_tag="PLE23_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein LysM"
                     /protein_id="HMX82795.1"
                     /translation="MGLFSFLKNAGAKLFGHKEEETKPTNVLTPEQQAEVDAIRKQTR
                     EAALVSEVVNMGLHVENLSVDLQGDTAIVYGQVDTQADKEKVILTVGNVDGVASVDDR
                     LEVANPEEDSQFHEVQKGDSLSKIAKHYYGDAMKYPIIFEANKPMLKDPDLIYPGQML
                     RIPPIA"
     gene            3480..4538
                     /locus_tag="PLE23_05710"
     CDS             3480..4538
                     /locus_tag="PLE23_05710"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HMX82796.1"
                     /translation="MYSSKIAGIGHYVPQQVVTNNDLSRYMDTTDEWVQERTGIKERR
                     YGLRHKETTTTMAVEAAKIAIERAGIDKNELDFIIFGTLSPDYYFPGCGVLLQRALGI
                     SGNTCGALDIRNQCSGFIYALSVADQFIRTGMYKNILVVGSEMHSMGLDFSTRGRNVT
                     VIFGDGAGVAVLQASDREGSGVLTTKLHADGTHAEMLSFISPGCHGGYHDEDKEKFGF
                     PDDEYGGLFITQKMIDDHLTYPFMDGQFVFKMAVQKFPEVINEALESVGLKKEDINML
                     IPHQANLRISQFVQRVLGLRDDQVWNNIQKYGNTTAASIPIALSEAWEAGKIKEGDLV
                     CLAAFGSGFTWGSALIRW"
     gene            4590..5921
                     /gene="rmuC"
                     /locus_tag="PLE23_05715"
     CDS             4590..5921
                     /gene="rmuC"
                     /locus_tag="PLE23_05715"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HMX82797.1"
                     /translation="MDNHYLLILLGVVFIFTLVIFKLLLDKNWTTKIQSGFVPKDQYN
                     QLSHSLTEKESVISALDQSLNALRIDITLKEEQLRSQDEMIDYKMREIEGIRETTRKE
                     FELLAQKIFEEKSSRFTETNKANIEILLKPLKENIQQFESRIHQQFTEEFKSRSELKK
                     EIELLKTHSDQMSREANNLAVAIKGDKKLLGDWGEIQLETILQHSGLEKNVHYKVQMS
                     IKDEEGNDKRPDFVVFLPDEKCIIIDSKVSLVAYEKYFNADADDERQKFLKQHIQDVR
                     NHIKGLATKSYQSLPGLQNPEYVLLFIPLEPAFNVVVQYDSRIFMEALDQNIVLVTST
                     TLMATMRTVYHIWRQQQQNMNAEKIARESGMLYDKFVGFVQDMTKIGDKIRESQGAYQ
                     DAMNKLTESSKKGDTIIGRVERIKKLGAKASKSIPGQLLADAGLQENTEGE"
     gene            complement(5924..7210)
                     /locus_tag="PLE23_05720"
     CDS             complement(5924..7210)
                     /locus_tag="PLE23_05720"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMX82798.1"
                     /translation="MLYALVRPVAKTGFKVFFRHIHIQGLENIPREGPLLLAANHPTA
                     FIEPCVAACFQHRTLHFIVRGDIFKNPFYIKILKALHLIPIFRFKDGYTNLKQNQETF
                     RYVNQVLQERKTILILVEGRTIQEKKLRPVQKGPARMAFGAYDAYGVKDTKIIPIGFN
                     YEQANAWRKDVMIQCGTPISVADFIPAYHENPLQAIQSLTDEIASGLAKNLVIIHDVQ
                     DEQACDILFEITKNDHSSPVFPIVIRNNERLITEKKIAAAFNNISEPEKSKLSTIANN
                     YRHQLEQYKIADANVITGKSAATFPSILLLIILQILFLPGALYYFIPVMTARMMTSKR
                     VYVKEFQAPVLFALSAFFVLIWSIFSLTSGIMLFGVRSIIMILILLLFGFLSIPVYEA
                     LMRNLSQRRFNRLQAETQQELFSSRNTMREWIQKNI"
     gene            7268..8146
                     /gene="miaA"
                     /locus_tag="PLE23_05725"
     CDS             7268..8146
                     /gene="miaA"
                     /locus_tag="PLE23_05725"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HMX82799.1"
                     /translation="MMIGNKYLYIIGGPTASGKTALAVNEAIRLNTVVISADSRQFYR
                     EMSIGTAKPSQQELDSVTHFFINNLSIHDEYDVGTYESEVIHLLDDLYKKHDIVIMAG
                     GSGLFIKAVTDGLDDFPEIESEVKEKCQLILDNEGIEGLQQFVRQKDPQYFAEVDIQN
                     PRRLLRAAEVILQSGQTYSGFRKKSRKIRNFATIKYLINPERELLYDRINLRVLQMVA
                     QGLEDEARQLYAYRHSKPLQTVGYQEWFDYFDGKITRNQAIELIQQNTRRYAKRQLTW
                     FRNDGGWSTDLPGILK"
     gene            complement(8291..>9220)
                     /locus_tag="PLE23_05730"
     CDS             complement(8291..>9220)
                     /locus_tag="PLE23_05730"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX82800.1"
                     /translation="TLDINTQLVPLGSGLKLTSGNHQIVFVSQTGCSDTVNIKVFCLD
                     PDDIHTTVQVSETKKVCIDNSQLQGTPVSITNICSDLGGEYSIIYASANDYCVSIAGI
                     EPGQEKACIVVCDDLGYCDTTYIYITVVESNFKPNAVDDYRSTLEGKPIIISVLDNDT
                     YGSGTVKVNLISKPDNATVSMYGTKLLTVTPQKGNCEPLEFQYAITNEFGSDTATVFV
                     NINCNDLVFNNGITPNHDGLNDVFFIEGIDKYPDNKLTVFNRWGNQVYSADKYKNDWD
                     GSWNSKMLPDGTYFYIFEDGIGNTYSGYIQILR"
BASE COUNT         2751 a         1634 c         1959 g         2876 t
ORIGIN      
        1 acagcgtgtt ttttgtcaac tgttacgccc gggaaattac ctgttttttg attcaaaccg
       61 gtgagtaggt tgaagattgt agtctttcca caattcggat tacctgctag tacaatgttt
      121 ctgttgccca aaatatacga ttatttcttg atgagaataa gttctgcttc ctgaagccgt
      181 agtccgatgt agtggttgtc cacatcaata tacaatgaac cttgccaagg ttgtcggcgt
      241 agcaacttaa tttttttgcc gggaatgata cccattgcta gtaatcttgt tgtaaaatta
      301 tgcactacaa gctcatcaat gatgcccatt tcgcccgcct gaagctcaga aagttttacc
      361 gtgttaccga gatttagatt catctttatt tagacttaat atgaataagc aaaattacag
      421 taaattgttc agtaagttaa attaatgtag attgaatttt tcagacatta aatctagtac
      481 aaattttagt tgttcttcct ttgttaggta tgaattatct agtaaaattg cgtctgctgc
      541 ctgtctgagc ggactgtctt ctctggtaga gtcgatgtgg tcgcggtgtg caaggttatt
      601 tttcacttct tcaaggctaa tgtcaatgcc tttttgttcg tattcttttt ttcgtcgagt
      661 ggctcttatt tcaggagatg cagtaaggaa aatttttaac tctgccgatg gaaacacgac
      721 agtaccgata tcacgaccgt ccatgacgat acctttttgc tgttccatgg cttgttgttg
      781 ttggactaga aagctcctta ctgaagatat ggccgacact tcgcttacga gttcactcac
      841 ttgcatggat cggatactgg tttctacgtc ttgactgttc aggaatgtat gaatttcgct
      901 cttaattttt cgaaaatcta ttgaaatgtt attcagtgca ttcgtcactt cttcttcatt
      961 tttaaaatcg atgttatggt tgatgaaata gagcgtaaca gcacgataca tggctccggt
     1021 gtcaatatat atataatgca gtacccttgc cagttcgcga gccagtgtac tttttccgca
     1081 agaagagtag ccgtctatgg caatgataat ctttttcaaa atctgcagat gaatatttgc
     1141 gccaaaataa ttaatttaat ttagacacaa gaagtttcca cagtttttcg taaggatata
     1201 tttcaagatc aaaatcttgc ccatgttcct tcatcggggt attagccagg ttatcgaggt
     1261 gtttttcgta acccgataag aatcttgtat tgaaattgct tttcactagc tgtacaagta
     1321 gtttgatgaa taaattgaca cgcaggtttt cttcaggtct tgttgggcgt gtggtgtatg
     1381 atttgagtcg ttcgacattc tcagcactta tcagaaaatt tttatttgaa tacgctacta
     1441 atgtttttgc tataaccgat tgtacggtgt agctgcggta ttctttggcg aagataagcg
     1501 ggtcagccat caatgattcg aataactgtg tcgatttttg tttgtaaatg ccctggaggt
     1561 tgttttgagt gttttcaatc atgaaataca agtaatattc atagattttc catttttcct
     1621 taagcattcg atgagctttt ctaaattttg gagaacttgt aacttttgaa tgtatggcca
     1681 gtgcattagc gaaattgtcg gaatgcatag ctgtaagaaa gtacaagtcc atgtatttat
     1741 gccaatgggc ggaaccctcc tcaaatactt ttacaaattt ttctacatct tttttggcat
     1801 tttcaaaatc tcggagattg agaaaggcgt tcagtttttt aatctggaag gtcaggagca
     1861 tatcgtcctg ttcgtattgc ggattgtgcg tgatgtaatc ctccatttta atacaaatct
     1921 ctatgacggc gtggaagtca ttttcatatt caaattttac caaccaagcg tagaacatat
     1981 taaatgtgat gacaggcgaa tcgtattttt ctgataataa aatgagttgg tcgcattggt
     2041 cgtcgagttc gttattcttg ctttctgaat gggttgaggt gttttcgtat cgaagtctga
     2101 caaactggtt aatttcttca gaacgcattt cagctaaaat gatatcctgg tattcattga
     2161 tgatttggtt gtaatactga aactcctttt catcacggtt gtgggcggca ttttgtctta
     2221 gaattcgggc actgttgaca ataatatctg cgaacttgaa tttgagtgca atggtcaata
     2281 tctgccctgc tatgtcatga gctgtgagtt cggcaccgta agagagcaat attttggcaa
     2341 gtgtccagtc tttattgcaa gaaaaataag ctctggtata attggaagcg gaaggcagat
     2401 ttatatctat aaaaaacagc gtattgagca gtctttttct aaaacgtgat ttgagttgtc
     2461 gatatttatc gtctgttggg ctgctttggt agaggagggc ggctgcatcc cggtctgttt
     2521 tgaatttgcc gtccataaga gcttcgaaaa attcgtttaa cttacttgat ttatttttta
     2581 aagacgcttc gtcaaatatt tcaatttttt ttaccttttt ccagcttaca atattagata
     2641 tctccagtaa atgtttcatt tggatgtgat tgttgttgta aaatatgaaa aaaatgtgac
     2701 attataaaat aagacaataa ttaagccaat gtgttcttta tcttatgtaa gtataagttt
     2761 tacaaacttc ggtttataat attgtattat aattgtgaca aacaaccttt ttaagatatt
     2821 gttaacgttt atttttgtgt aaaatttgtt atctttgtgc gaattaattt tttcattaaa
     2881 tttcaaaaca attatgggac tattttcatt tttaaaaaat gccggtgcaa agctttttgg
     2941 ccacaaagaa gaagaaacaa aaccgactaa cgttcttact cctgaacagc aggcagaagt
     3001 ggatgcaatt agaaagcaaa cacgcgaagc agctcttgtt agcgaggtag taaatatggg
     3061 cttacatgtt gaaaatctat ctgttgacct acaaggtgat accgccattg tttacgggca
     3121 ggttgatacc caggctgaca aggagaaggt tattcttaca gtcggtaatg ttgacggtgt
     3181 tgcatctgtt gatgacagac ttgaagtagc taatcctgaa gaagattctc agtttcatga
     3241 ggtacagaaa ggtgactctc tgagcaaaat tgcaaagcat tattatggtg atgctatgaa
     3301 gtatcctatt atttttgaag caaataagcc aatgttgaaa gatcctgatc ttatctatcc
     3361 tggtcagatg ttgagaattc ctccaatagc ataagacata aattatttgt aaaatggctg
     3421 tcagattttg acagccattt tttttgtgtt atttttggag aattttaaaa gcaactgata
     3481 tgtattcttc aaaaattgcc ggcataggcc attatgtccc acaacaagta gtgaccaaca
     3541 acgatctatc cagatacatg gatacaaccg atgaatgggt gcaagaacgc acgggtataa
     3601 aagaaagacg ctatggactc cgacataaag aaacaaccac aacaatggcc gttgaagctg
     3661 caaaaatagc catcgaaaga gctggtattg acaaaaacga gcttgatttt atcatttttg
     3721 gcacgcttag tccagattat tactttccgg gttgtggtgt attgctccaa agggcactcg
     3781 gcatatcggg taatacatgt ggtgcactag atattcgcaa tcaatgttct ggttttattt
     3841 atgccttgtc agtagctgac cagtttatcc gtacaggtat gtataaaaat atccttgttg
     3901 taggatctga aatgcacagc atggggcttg atttttcgac acgcggccgt aatgtcaccg
     3961 ttatttttgg agacggtgca ggtgtagcag ttttgcaggc atccgacaga gaagggtctg
     4021 gtgtgcttac cacaaaactc catgcagacg gcacacatgc tgaaatgctg tcttttataa
     4081 gcccgggttg ccatggtggt tatcatgatg aggataagga aaaattcgga tttccggatg
     4141 atgaatacgg tggtttgttc attacccaaa agatgattga tgatcatttg acctatccat
     4201 tcatggatgg acaatttgta tttaaaatgg cagtacaaaa atttcccgaa gtaatcaatg
     4261 aagccctaga gtctgtaggt ttgaaaaaag aagacatcaa tatgctcatt ccgcatcagg
     4321 ccaatttgcg tatcagtcaa ttcgtacaga gggtacttgg ccttagagat gaccaggtat
     4381 ggaacaacat tcagaagtat ggtaatacca cagcggcctc tatacctatt gcattgtctg
     4441 aggcatggga agctggtaag ataaaggaag gtgatctggt ttgtctggct gcatttggca
     4501 gtggatttac atgggggtcg gctctcatcc gctggtaata catatagggt tattgctcgg
     4561 gtcagattta ttttttaatg taaaaatgta tggataacca ttatttgctt attctactag
     4621 gcgttgtatt catttttacc ctggtaatct ttaaattatt gctagataaa aattggacta
     4681 caaaaataca aagtggattt gtacctaaag atcagtacaa tcaattaagc cattctttaa
     4741 ccgagaaaga atctgtgatc agcgcacttg accaatcatt gaatgcattg aggatagata
     4801 ttacactcaa agaagagcaa ctgcgtagcc aagatgaaat gattgattac aaaatgagag
     4861 aaattgaagg tatccgtgaa acaacccgca aggaattcga actgctggca caaaaaatat
     4921 tcgaagaaaa atcaagccgt tttactgaaa ccaataaagc taatatcgaa atcttgctca
     4981 agcctctaaa agaaaacata caacagtttg aatcaaggat tcatcaacag ttcacagagg
     5041 agtttaagag ccgatcggaa ctgaaaaagg agatagaatt attaaagacc cacagcgacc
     5101 agatgagcag agaagctaat aaccttgcag tcgctatcaa aggagataaa aaattactcg
     5161 gagactgggg cgaaattcag ctcgaaacca ttttgcagca ttcgggtctg gaaaaaaatg
     5221 tccattacaa ggtacagatg agtattaagg atgaagaggg aaatgataaa aggccggact
     5281 tcgttgtttt tcttcctgac gaaaaatgca taatcatcga ttcaaaagtg agtcttgttg
     5341 cttatgaaaa atattttaat gctgatgctg atgatgaaag acaaaagttt ctcaagcaac
     5401 atatacagga tgtcagaaat cacataaagg gtcttgctac aaagtcatac caaagccttc
     5461 ccgggcttca aaatccagaa tatgtattgt tgtttattcc gttagagcct gcattcaatg
     5521 ttgttgtaca gtatgacagc cggatattta tggaagctct tgatcagaat attgttcttg
     5581 tcacttcaac tactttgatg gccacaatga ggactgtgta tcatatctgg cgacagcaac
     5641 aacaaaatat gaatgccgag aaaatcgccc gtgaaagtgg catgctatat gacaaatttg
     5701 taggctttgt acaggatatg accaaaatag gtgataaaat cagagaaagc cagggagctt
     5761 accaggatgc gatgaataaa ctgacagaat cttcgaaaaa gggcgacacc atcataggca
     5821 gggtagagcg tatcaaaaaa ctgggcgcta aggcaagcaa gtctattccc ggtcagttat
     5881 tggcagatgc aggtttgcag gaaaatactg aaggcgaata acttcagatg tttttttgta
     5941 tccattcccg catagtgttt ctgctagaga aaagttcttg ttgggtctct gcttgtaatc
     6001 tgttgaacct tcgttgagac aaattcctca tcaatgcctc atagacaggt atggataaaa
     6061 atccaaacag caacaagatg aggatcatga tgatactcct gacaccgaac agcatgatac
     6121 cgctagtaag agaaaaaatg ctccatataa gtacaaaaaa ggcagacaga gcaaacaaaa
     6181 caggtgcctg aaattctttt acatatacac gctttgaagt catcattctt gctgtcatca
     6241 ccggtatgaa atagtataat gcacccggca aaaagagtat ctggagaata atcaaaagca
     6301 aaatagaagg aaacgtggct gcggattttc cagttattac attggcatca gcgattttgt
     6361 attgttcaag ttggtgacgg taattgttag caatagttga aagtttactt ttttcgggtt
     6421 cgctaatatt gttgaaagcc gcggctattt ttttctcagt tataagtctt tcgttgttgc
     6481 gtatcacaat gggaaatacc ggagaagaat ggtcattttt agtaatttcg aataagatat
     6541 cacatgcctg ttcgtcctgt acatcatgga tgattacaag gtttttggcg agcccggagg
     6601 cgatttcatc cgtcagcgat tggatggcct gcaaagggtt ttcatggtat gccggtataa
     6661 agtcagccac tgaaatgggt gttccacatt gtatcatcac atcttttcgc caggcattag
     6721 cctgttcgta gttaaatccg atgggaataa tttttgtgtc ttttacacca taagcgtcat
     6781 aagcgccaaa tgccattctt gccggtcctt tctgtacagg acgaagtttt ttttcctgaa
     6841 tggttcttcc ttcaacaaga attaaaatgg tttttcgttc ttgcagcact tggtttacat
     6901 acctgaaagt ttcttggttt tgcttaagat ttgtatagcc gtccttgaat ctaaaaatag
     6961 ggatcaggtg aagtgccttg agaattttaa tgtaaaaggg atttttgaaa atatcaccac
     7021 gcactataaa atgtagagta cggtgctgga agcaagcagc gacacaaggc tcaataaatg
     7081 cagtagggtg gttagctgca agtaaaagtg gcccctcacg gggaatattc tcaagcccct
     7141 gtatatggat atgacgaaaa aaaaccttga agccggtttt agcaaccgga cgcaccagag
     7201 cgtacaacat tttgctgatt gtttttaatg aattcttaca caaatatggt caattttgta
     7261 gattcgtatg atgattggta ataaatatct gtatataatc ggtggaccga ctgcatcagg
     7321 caaaacggca ctggctgtta atgaggcaat tcggcttaac acagtggtta tttctgccga
     7381 cagccggcag ttttatcgtg aaatgtcaat cggtacggcc aaaccatctc aacaggaact
     7441 ggatagtgtg acacattttt ttatcaacaa tctgtctatc catgatgaat atgatgtagg
     7501 aacatacgag agcgaagtaa tccacctgct tgatgatttg tacaagaagc acgacatagt
     7561 gattatggct ggtggttctg gactcttcat aaaagcagtg acagacggac tcgatgactt
     7621 tcctgaaata gaaagtgaag taaaggaaaa atgccagctt attttagata atgaaggcat
     7681 agaaggattg caacaattcg tcagacaaaa ggatccacaa tattttgctg aggtagatat
     7741 acagaatccg aggcgattgc tcagagctgc agaagtaatt ttacagtccg ggcagactta
     7801 ttccgggttc agaaagaaga gccggaaaat cagaaacttt gcaactatta aatacttgat
     7861 taatccagaa agagaattat tgtacgatag aatcaatcta cgtgtgttgc aaatggtagc
     7921 gcaaggtcta gaggatgaag cacgccaatt gtatgcatac cgtcactcga aaccgctgca
     7981 gacagtaggt tatcaggagt ggtttgatta ttttgacgga aaaattaccc ggaatcaagc
     8041 gattgagctc attcagcaaa ataccagacg ttatgctaaa agacagctga cctggttcag
     8101 aaatgacggt ggctggtcaa ctgatttgcc gggaatattg aagtgatgta ataacctgat
     8161 tcacttaatc aagtaatgat tattagcttg gattttgtat tggctcattt ccgaaggttt
     8221 tgaaaaaaaa ggcggcagtc actttcgtaa ctgccgctgc ctcaaaaagt ttaagtcctt
     8281 atctttattc ttatctcaga atctgtatgt aaccggagta agtatttccg ataccatctt
     8341 cgaagatata gaagtatgtt ccgtcaggta gcattttgct gttccaactt ccgtcccagt
     8401 cgtttttata tttatcggct gagtaaacct gattgcccca tctgttgaac acggtcagtt
     8461 tattgtcagg atatttgtca attccttcaa taaagaacac atcgttcagt ccatcatggt
     8521 taggagtaat accattattg aacacaaggt cgttgcagtt tatgttaacg aaaactgtag
     8581 cagtatcgct tccaaattca tttgtaatgg catactggaa ctcaagtggt tcacagttac
     8641 ctttctgtgg tgtaacagtt aacaacttag taccatacat gctcacggtt gcattgtccg
     8701 gtttgctgat tagattcact tttaccgtac cagatccgta tgtatcgtta tcaagtacgc
     8761 ttatgataat cggcttacct tcaagggttg atctgtaatc atcaacagca tttggcttaa
     8821 agttagactc tactactgtg atgtaaatgt atgttgtgtc acagtaaccg aggtcgtcac
     8881 agaccacgat gcatgctttc tcctgtccag gttcaatgcc tgcaatgctt acacaatagt
     8941 cattagctga ggcgtagata atactgtatt cgcctcctag atcagagcaa atattggtaa
     9001 tgcttaccgg tgtgccctgt agctggctat tgtcgataca tactttctta gtttcagaaa
     9061 cctgaacagt tgtgtgtata tcatcaggat ccagacagaa taccttgata tttaccgtat
     9121 ctgagcaacc ggtctggctt acgaatacga tctgatgatt gccggatgta agtttcaagc
     9181 ctgaaccgag tggtacaagt tgtgtattga tatcaagtgt
//
LOCUS       DAOQQY010000086         9198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2830_length_9198_cov_6.131467, whole genome shotgun sequence.
ACCESSION   DAOQQY010000086 DAOQQY010000000
VERSION     DAOQQY010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2830_length_9198_cov_6.131467"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLE23_05735"
     CDS             complement(<1..411)
                     /locus_tag="PLE23_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82801.1"
                     /translation="MPKVNKYILPEKWVTYIQLFWGVWFVCMMIFTKYGTSMSIIGFA
                     VLGFYAVVTHFSLPAGEYFSKCFSVNNRSLGFLALTLVFWITVIGFFNSDNTQYWLSR
                     VRIRLPFLALPVVFYWIPWNNRQKRIIVLTAMLMM"
     gene            534..1559
                     /gene="pheS"
                     /locus_tag="PLE23_05740"
     CDS             534..1559
                     /gene="pheS"
                     /locus_tag="PLE23_05740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMX82802.1"
                     /translation="MEVFDLIASMQNEISAAAPANSEQLEQFRIRFLGSKNVIKPLFA
                     EMKNVPNDRKKEFGQLLNSLKESAEEKFKSVQESFESNADEEYDLDVTAPGFPLIQGT
                     RHPISLAMERIINIFTKIGFVIAEDREIEDDWHNFTALNTPDDHPARDMQDTFYLQGS
                     TEQLLRTHTSSVQTRVMTSHKPPIRIIAPGRVYRNETISARSHCQFHQIEGLYIDKNV
                     SFADMKQTLLYFAREMYGPDTRIRMRPSYFPFTEPSAEVDVYWGLKDETDYRITKGTG
                     WLEVLGCGMVDPAVLNNCGIDPEEYSGFAFGMGIERQAMLLHRIGDIRQFFENDVRFL
                     KQFSAVL"
     gene            1571..2911
                     /gene="hisS"
                     /locus_tag="PLE23_05745"
     CDS             1571..2911
                     /gene="hisS"
                     /locus_tag="PLE23_05745"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMX82803.1"
                     /translation="MKPSLPKGTRDFGPDQVLKREYIFNIIKEVFRLFGFAPLETPAM
                     ESLSTLTGKYGEEGDRLLFKILNNGNFLEGADEKALNEKNSQALVSSISKRGLRYDLT
                     VPFARYVVMNQSNLSFPFKRYQIQPVWRADRPQRGRYQEFYQCDVDIIGSDHLMYESE
                     LIQIYDLVFCKLELPVIIRINNRKILYGIAEKAGITDKFIKMTTALDKLDKVGWEGVT
                     KELESAGITPSQTETIKTISATTDLNLLERLLDGCEVGEKGCSELKEVLRYAGESGLA
                     NKLQFDITLARGLSYYTGCIIEVVAADANMGSIGGGGRYDDLTGVFGMQGMSGVGVSF
                     GAERIYDVLEEKKLFPENLGVTLNLLIVSFDEVCFQAGVTMVRDLRKAGIQCDIYPGA
                     PKMQKQMKYANDRKLPYVIIIGSEEMASGLFTVKDMMTGTQEKLTFAEISERFS"
     gene            3167..4621
                     /locus_tag="PLE23_05750"
     CDS             3167..4621
                     /locus_tag="PLE23_05750"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMX82804.1"
                     /translation="MRKYLLALMLILSCEFVTGAATPVVPGLSDIGVEPEGGAADGDM
                     RTKIQSLNIPFTPKYTRELKSKIDDYIRIYRKKTSRMLGLTSIYFPIIEEYLKASGIP
                     DVFKYLPIIETDLNPRAVSPDGAVGIWQFMPSTGAIYGLSSDKFVDQRCDIHRSTEAA
                     AKYLKKLYDIYGDWALVLAAYNCGPGRVNSAIKKGLSNNYWVIEKYLPEQTRNYIPNF
                     IATAYAMQYFPEYGITADNPDLDLQLTKEMKVYKELTFDEISLVTGLDVSQIAILNPQ
                     YLRNFIPASTDGNFLTLPKRIINAFNHFVSLPDNRKNDYLKVAPIINEERDLNTSFYH
                     KTNIIAGSGESLKDIAEQYDISVHSLVVWNNITELTLASGTEVAVYLPKKTEVKITEV
                     PQSRKLKSFKLTSRQVDQPLIGDAILDLKHYVNFAPTEYVTYIMLPEESLQDIATKYG
                     VTIQDLITLNQFDDEWTPKPGMIIRIKESEIIKP"
     gene            complement(4675..5115)
                     /locus_tag="PLE23_05755"
     CDS             complement(4675..5115)
                     /locus_tag="PLE23_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMX82805.1"
                     /translation="MKLIRPNYSPVKDVFQDFFSNAVNRTLSDILNIDSPISQPSVNI
                     RETPEKFEIELAAPGLEKNDFKLALENNRLSISAQKEERKVEENGNYSRKEFSFNSFT
                     RSFDLPETVHAENISANYENGILYINLPKKEEAKPQPAKQIEIK"
     gene            complement(5145..5585)
                     /locus_tag="PLE23_05760"
     CDS             complement(5145..5585)
                     /locus_tag="PLE23_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMX82806.1"
                     /translation="MTTLFFAPYQMNCKTAGCAPDNTRTQHYRHPMHSNKNFNTPPAN
                     IIKGEKGYELQLSLPGFSKENINIQVAENTLNIEGRINPTVSENEKVLRKEFSSKDFK
                     RSFTIGDVLDANTIDAAFQNGILSIKIAFKPTPEPQKPNTIEVK"
     gene            5954..6724
                     /locus_tag="PLE23_05765"
     CDS             5954..6724
                     /locus_tag="PLE23_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HMX82807.1"
                     /translation="MIIRLNTILSSTSYRHTKFLGLLFPILQLCFWYIPASYAQENDT
                     LYRYIDVTGNAELEVLPDQIILAITIEEYLKEDLDQNTQYRNYHKKVDIAILEQNLIT
                     DLKKLGIGRERISQRQSPGQWRNMQYGEDAMQSRVYYINIQNMNERDRIFTTLHTAGI
                     TSISTKELKSNNLAEYNKQVMVEALKDAQNKADLLLRSIGAKTGPVISISEIEDTRNT
                     ADNRSNEDKHTGMYLFTQTEEKRAINLRFALRVRFRIQ"
     gene            complement(6737..7276)
                     /locus_tag="PLE23_05770"
     CDS             complement(6737..7276)
                     /locus_tag="PLE23_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMX82808.1"
                     /translation="MKKILLSFFVALFACSSMVSAQKVKKNVQPDKPKDFLPIYRNPQ
                     VQAMFPGCEGTVDEYRLGCSQQKLTGYIKSVMKYPKSLARDTIEGMSFVSLVIDTSGT
                     VSHIRIDKPGTHPKFDKEALRIAKTFNKMKTKWLPAMQNGKKVMSEKVIPVKFTIRKP
                     KLMVAPPDEDDPNQDTNNK"
     gene            complement(7289..8023)
                     /locus_tag="PLE23_05775"
     CDS             complement(7289..8023)
                     /locus_tag="PLE23_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82809.1"
                     /translation="MNKRILLMLTLFAVGFQAKLLQAQGYAFGLKGGLTAAFQTWDNY
                     GRDALYKPHGILFIESADDDAGSLFAQIGYHIKGAANRPKGRTIYRDLQGNLIEYKPS
                     VIEYQFHNLSLTAGFKKKYGFGSNFAYYLLGLRGDYTLKTNFDQYKEINKYYFFYPDD
                     VYVKKWNYGVTVGGGFEFPFSELVRGIVEFTVNPDFSKQYRQPPITNIINGLGQVYNV
                     PERQISNVTIELTVGMRFMNKIEYID"
     gene            complement(8016..>9198)
                     /locus_tag="PLE23_05780"
     CDS             complement(8016..>9198)
                     /locus_tag="PLE23_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMX82810.1"
                     /translation="SCFRKYNNAEESFIDHSEFIRRPNGPYQSLFNLDPKDYKAWAYG
                     LKKAGYATASDYPQKLIGVIERYQLYQFDGGTPIKTDPSQQPVVVNEPKKSKEPIFTK
                     KEKPIRINMVKAYIAKGGEKVSDVASKYDVPESYILAYNDYLKSPNQLLKENDIVYLQ
                     KKKKSFWGKKKIHYVTAGQTIFDIAQQYGVREENLRERNMLNMVDQPDAGQKVYLRGK
                     LPKNEKIKTIKAMPVQTETPKSAVDTLAISSPNAETKTNPSQVQVKQTPKEPANGNND
                     LLDFYVVPQEKKSDSTQTAKPDTSTFKPAITGVNVPSTPVVTKVQTTQQDPVTKTEQA
                     AQPNKNQSPGTDFYHTVDKGDTLYNISKRYNTTVAKIKEINQLASDYITIGQRLLINH
                     E"
BASE COUNT         2726 a         1698 c         1972 g         2802 t
ORIGIN      
        1 catcatgagc atggctgtca gtacaatgat gcgcttttgt ctgttgttcc atggtatcca
       61 gtaaaatact accggcaatg cgagaaacgg gagtctgatg cgtactcttg acagccagta
      121 ttgtgtatta tctgagttga aaaacccgat gacagtaatc caaaatacaa gagtgagtgc
      181 aagaaagcca agcgaccgat tgtttacaga aaagcatttt gaaaaatatt cgccggcagg
      241 caagctgaaa tgagtaacta ctgcataaaa tccaagcact gcaaagccga tgatactcat
      301 agaagtgccg tatttggtga aaatcatcat acacacaaac caaacacccc agaataactg
      361 aatgtacgta acccattttt ctggaagaat gtatttgttt acttttggca tgaggataaa
      421 atgatgtttt atgacaaatt attgcattgc ttgtatcaaa taagaaagag tcggcgtatt
      481 aacgattaca aaattaaaaa ttttaccttt gcacttcatt tatcatacaa attatggaag
      541 tttttgatct gattgcttca atgcaaaacg aaatatctgc tgctgcacct gccaacagtg
      601 aacaacttga acaattccgc attaggtttt tgggcagtaa aaatgtcatc aagccattgt
      661 ttgccgaaat gaagaatgtg cccaatgacc gtaaaaaaga attcggtcaa ctgcttaatt
      721 cactgaagga aagtgcagaa gagaagttca aaagtgttca ggaatcattc gaaagtaatg
      781 ctgatgaaga gtatgacctc gacgtgacag cacccggatt cccactgata caaggtaccc
      841 ggcatccgat ttcacttgct atggagcgca tcatcaatat atttaccaag atagggttcg
      901 taatagctga agatcgtgag atagaagacg actggcacaa ttttacagca ctcaatacac
      961 ctgatgacca cccggcacgc gatatgcaag acacatttta cctgcaagga agtacagaac
     1021 aactgttacg cacgcacact tcttcggtac aaacgcgggt aatgacatca cacaagccac
     1081 ctatacgcat cattgcaccg ggcagggttt accgcaatga gaccatctca gccagatcgc
     1141 actgccagtt tcaccagata gaaggattgt atatagacaa gaatgtatca tttgctgaca
     1201 tgaagcagac tttgctttat tttgcaagag aaatgtacgg acccgatacc agaatccgta
     1261 tgaggccgtc ttatttcccc tttacagaac caagtgccga agtggatgtt tactgggggc
     1321 ttaaggacga aaccgactat cgtatcacta aaggtacagg ttggctcgaa gttcttggat
     1381 gtggaatggt tgacccggca gtactgaaca attgtggcat tgaccccgaa gaatatagcg
     1441 gatttgcatt tgggatggga atagaacgac aggccatgct gctgcacaga ataggcgata
     1501 tacgccagtt ttttgagaat gatgtcaggt ttttaaagca attttcagcc gttttataaa
     1561 tattcaatac atgaaacctt cgttgccaaa aggtacaaga gatttcgggc cagatcaggt
     1621 tctgaagaga gaatacatat ttaatataat aaaagaagta ttcagactgt tcggttttgc
     1681 cccactcgaa acacccgcca tggaaagcct ctcaaccctt acaggtaaat atggtgaaga
     1741 aggtgacaga ctactgttta agatactcaa taatggaaat tttttggaag gtgctgatga
     1801 aaaggcattg aatgaaaaga attcgcaggc cttagtgtca tctatttcga aaagagggct
     1861 acgctacgat ctcacggtac catttgcacg ttatgtagtg atgaatcaaa gcaatctttc
     1921 atttccgttt aaacgctacc agatacagcc tgtgtggcgt gctgacagac cacaacgagg
     1981 caggtatcaa gagttttatc agtgtgatgt ggatatcata ggcagtgacc acttgatgta
     2041 cgagtcggag ttgatacaga tatacgacct ggtattctgc aagctagagt tgccggtgat
     2101 tatacgaatc aataaccgta agatattgta cgggatagca gagaaagcag gtattaccga
     2161 caaatttata aaaatgacta ccgcgctcga caaactcgac aaagttggtt gggaaggtgt
     2221 gactaaagaa ctagaaagtg ccggtataac accatcacag acggagacga taaaaaccat
     2281 ttctgcgact accgacctca acctgcttga gcgtttgctt gacggatgtg aggtaggcga
     2341 aaaaggatgt agcgaactga aggaagtgtt gcggtatgca ggcgaatccg gccttgccaa
     2401 taaactacag ttcgatatta cacttgccag agggctgagc tattacacgg gctgcataat
     2461 cgaagtagtg gcagccgatg ccaatatggg tagtatcggt ggtggaggaa ggtacgatga
     2521 ccttaccgga gtatttggta tgcaaggtat gtcaggtgta ggggtttcgt ttggggcgga
     2581 gcgaatatat gatgtgcttg aggagaaaaa attatttccc gaaaacctcg gagttacatt
     2641 aaacctactc attgtcagtt ttgatgaagt ttgttttcaa gccggtgtaa cgatggtcag
     2701 ggatctaaga aaagcaggca tacaatgtga tatttacccg ggtgcaccaa agatgcagaa
     2761 gcagatgaaa tatgccaatg accgaaaatt accttatgtc atcattattg gttcggaaga
     2821 gatggcatcg ggcttgttta cggtgaaaga tatgatgacc ggcacgcaag agaagcttac
     2881 atttgctgaa ataagcgaaa gattttcata gaattttaag caaataatat tttacaaaca
     2941 agcgcaggct gaggaatcat tttggctgct tcatgctatg aaacagtaat gtttattttt
     3001 tttgagtggg ttaaatattt caaaaatttg actatgtatt ccttgatttt ttcaaagaca
     3061 tatacataat aaaaatagaa attttgttaa tattctgtca aggactacct aaatagaata
     3121 ttatttgtat ttttgcgctg caatttaaag acgaaaacaa aattacatga ggaaatatct
     3181 gttggctttg atgctgatat tgtcatgcga gtttgtgacc ggagctgcaa cgccggttgt
     3241 gccgggcctt tctgatattg gtgtagagcc agaaggaggt gctgctgatg gcgatatgcg
     3301 aactaagata caaagcctga atataccatt tacacctaaa tatacaaggg agttaaagag
     3361 caaaattgac gattatattc gtatatatag aaaaaagacc agtagaatgc taggtctgac
     3421 gtcaatttat tttccgatta tagaagaata tctgaaagcc agtggcatcc ccgatgtgtt
     3481 taaatatctg cccatcatcg aaacagactt gaacccacgt gcagtgtcac ctgacggtgc
     3541 agtcggcatt tggcaattca tgccttcgac aggtgccatt tacgggctta gttctgacaa
     3601 attcgtagat cagagatgtg acatacacag gtcaaccgaa gcagcagcaa aatatttgaa
     3661 aaaactgtat gacatatatg gcgactgggc acttgtactt gcagcttaca attgcgggcc
     3721 gggtcgtgtc aattctgcta tcaaaaaagg cttgagtaat aactattggg ttatagaaaa
     3781 atatctacct gagcaaacca ggaattacat cccgaacttc attgctacag catacgctat
     3841 gcagtatttc ccggagtatg gtataactgc cgataatcct gacctcgacc ttcagctcac
     3901 caaggaaatg aaagtatata aagaattaac tttcgatgaa atcagccttg tgactggtct
     3961 tgatgtaagc cagatagcta tacttaatcc acagtatctc aggaatttta tccctgctag
     4021 tacagacggc aatttcctta ctttgcctaa gagaatcatc aatgcattta accatttcgt
     4081 tagtctgcct gacaacagaa aaaacgatta tctaaaggtg gcacctatca tcaatgaaga
     4141 aagagacctc aatacttcat tttaccacaa aacgaatatc attgctggtt cgggtgagtc
     4201 gctaaaagat attgctgagc aatacgatat aagtgtacat tcgctcgtgg tgtggaataa
     4261 tattactgag ttgacacttg catccggaac agaagtggca gtatatttgc ctaagaaaac
     4321 agaggtaaaa ataaccgaag tgccacagtc aagaaagttg aaatctttta aattaacgag
     4381 ccgccaggtt gaccagccac taataggaga tgcaatccta gacctaaagc attacgttaa
     4441 ttttgcaccg accgaatatg tcacctacat aatgttgccg gaggaaagcc tgcaggacat
     4501 agctacaaag tatggtgtta ctatccaaga tctgataaca ctcaatcagt tcgatgatga
     4561 atggacacca aaaccgggca tgatcatccg tatcaaagag tcagaaataa taaagccttg
     4621 attttttagg tcaaggctta catctaaaac taactaacta atcaacttac ttttttattt
     4681 gatttcaatc tgtttggcag gttgaggttt tgcttcttct ttttttggca aattgatgta
     4741 aagaatcccg ttttcgtagt ttgctgaaat gttttctgca tgtacggttt caggtagatc
     4801 gaatgaccgg gtgaaagagt tgaagctgaa ctcttttctt gagtaatttc cattttcttc
     4861 aactttgcgt tcttccttct gagcagatat actcagacgg ttgttttcaa gtgcgagttt
     4921 gaaatcgttt ttttcaagtc ccggagcggc tagttctatt tcaaatttct ccggagtttc
     4981 gcgaatgttg acacttggct gagagattgg gctatcaatg ttcagaatgt cggacaatgt
     5041 tcggttaacg gcattgctga aaaaatcctg aaatacatct ttcacagggc tgtagtttgg
     5101 tctaatgagt ttcatttttt taaggttttt ttgtgtgtta ataattattt tacttctatt
     5161 gtgtttggtt tttgaggctc aggtgtaggc ttgaatgcta ttttaatact aagtatgcca
     5221 ttttggaatg ctgcatctat tgtattagca tctagcacat caccaatggt aaagcttctt
     5281 ttgaaatcct ttgatgaaaa ttctttacgt agaacttttt cgttttcgct tacagtcggg
     5341 tttattctgc cttcaatgtt gagcgtgttt tctgctacct gaatgttgat attttcctta
     5401 gaaaatcctg gcagactcaa ttgtagttcg tatccttttt cgcctttaat gatgttggca
     5461 ggtggtgtgt taaaattttt gtttgaatgc attggatgtc ggtaatgctg tgtacgtgta
     5521 ttgtcaggag cacatccggc agttttgcaa ttcatctggt aaggggcaaa aaacaatgtt
     5581 gtcattttat taatttttta gtttattttg taaatacatt tctcttcata tctctcaatt
     5641 agcattccat tccgattttt cggtcataag tgcagatttt tcatcatttc aacatgccat
     5701 aatgacatat ttgcttaata aaatatgcca taatggcata ttggtaagat tcattgtaca
     5761 atagaaaaca tcaaatcaaa ctttgcaagg aagcccgttc gaataagata gtcggttttg
     5821 caaggtagta atagaaaggt ataattttag aaaagcacat caaaatgaaa aaacaacggg
     5881 tgtatcaagg catattttga aaatatttaa aaaaacaaca tgagttttgt acgaaattgt
     5941 ctaattttaa cgaatgatta ttaggttgaa cactattttg tcttccacaa gctataggca
     6001 cacaaagttc ttgggcttgt tatttcccat tcttcagttg tgtttttggt acataccagc
     6061 aagttatgca caggagaatg atactttgta ccgatatatt gatgtaacag gtaatgcaga
     6121 gctggaagta ttgcccgatc aaatcattct agccatcacc attgaagaat atcttaagga
     6181 agacctggat caaaatacac aataccgtaa ttatcacaag aaagtggata ttgccatact
     6241 tgaacagaat ctcataacgg atttgaaaaa actaggtatt ggtcgagaga gaatttcaca
     6301 aagacaatca cccgggcagt ggcgaaacat gcagtacggc gaagatgcca tgcagagcag
     6361 agtttactac attaatattc agaacatgaa cgaacgtgac cgtatattta ctacgctgca
     6421 cactgccggt attacaagta tcagtaccaa agaactaaag tccaataatc tggcagaata
     6481 caacaagcaa gtgatggttg aagcactcaa ggacgctcaa aacaaagccg atcttttgct
     6541 caggtctatt ggtgccaaga cgggtccggt catcagtatt tctgaaatag aagacaccag
     6601 aaacacagct gacaatcggt ctaatgaaga taaacacaca ggtatgtact tattcactca
     6661 gacagaagag aaacgggcaa tcaacctcag gttcgcttta agggtacgtt tcaggataca
     6721 ataatctagg tgcaaattat ttattgttag tatcctggtt tggatcatcc tcatcaggag
     6781 gtgccaccat tagctttggt ttgcggatgg taaattttac agggatcacc ttttcagaca
     6841 ttactttttt tccgttttgc atagccggaa gccattttgt tttcatctta ttaaatgttt
     6901 tggctatgcg caatgcttcc ttatcaaatt ttggatgtgt acctggctta tcaatgcgta
     6961 tgtggcttac ggtgccggag gtatcaatca ctaatgatac aaatgacatg ccttcaatgg
     7021 tgtcacgagc cagtgatttt ggatatttca ttacagattt gatatatcct gttagttttt
     7081 gttgcgaaca acccagcctg tattcatcta cagtaccttc acagcccgga aacatagcct
     7141 gtacctgagg attcctgtaa ataggaagaa aatcttttgg tttgtcgggt tgtacatttt
     7201 tctttacttt ttgtgcagaa accatactgc tgcatgcgaa taaagccaca aagaatgata
     7261 gaagtatttt tttcatgttt aaatattatt agtcgatgta ttctatttta ttcataaatc
     7321 tcatacctac ggtaagctcg attgttacat tgcttatctg gcgctcgggc acattatata
     7381 cctgacccaa accgttgata atgttggtaa taggaggttg tctgtattgt tttgaaaaat
     7441 ccggattaac cgtaaattcg acaatgcctc ttaccagttc ggagaacggg aattcgaaac
     7501 ctccacctac agtaacgccg taattccact ttttgacata aacatcatca gggtagaaga
     7561 aataatattt gttgatttct ttgtattggt cgaagttggt tttcaaagta taatcgcccc
     7621 ttagtccgag caaatagtaa gcaaaattcg aaccaaaccc atattttttc ttaaaacctg
     7681 ctgttaagga aaggttatga aactggtatt caatgactga gggtttgtat tcgatgagat
     7741 tgccctgcag gtcgcgatag atggtacgtc ctttgggtcg gttggcagca ccttttatat
     7801 ggtagccaat ctgagcgaac aacgatccgg catcatcgtc tgccgattcg ataaacagga
     7861 tgccgtgtgg tttgtaaagt gcatctcgtc catagttgtc ccaagtctgg aaggctgctg
     7921 tgagccctcc tttgagcccg aaggcataac cctgtgcttg taatagctta gcctgaaagc
     7981 ctacagcaaa aagtgtaagc attaaaagta ttcttttatt catgatttat caataagcgt
     8041 tggccgatag taatataatc tgatgcaagt tggtttattt ccttgatttt cgcaactgtg
     8101 gtattatatc tttttgaaat attataaagt gtatcgcctt tgtccacagt atgataaaag
     8161 tcggtgccgg ggctttgatt tttgtttggt tgagcagctt gttctgtttt ggttactggg
     8221 tcttgctgtg tggtttgtac ttttgtaacc accggtgttg aaggaacatt gacacctgta
     8281 atggcaggct tgaatgttga tgtatccggt ttggcagtct gagtgctgtc tgattttttc
     8341 tcctgaggta ctacgtaaaa atcaagcaaa tcattattgc cattagccgg ctccttagga
     8401 gtttgcttta cttgcacctg tgacggattt gtttttgttt cagcattggg agaggaaatt
     8461 gccagtgtat caacagcaga ttttggtgtt tctgtctgta caggcattgc tttgatggtt
     8521 ttaatttttt cattttttgg cagtttgcct ctcagatata ccttttgtcc ggcatcgggt
     8581 tggtccacca tattcagcat attgcgctct cgtaagttct cttcgcgtac accgtactgt
     8641 tgggcgatgt caaaaattgt ctgaccagct gttacataat gtatcttttt tttgccccag
     8701 aaagatttct tctttttctg gagataaaca atatcatttt cctttaacag ttgattgggg
     8761 gacttgaggt aatcgttgta ggcaagtata tacgattcgg gcacatcata ttttgaggct
     8821 acatctgata ccttctctcc tccttttgca atgtaagcct tcaccatatt gattcggatg
     8881 ggtttttctt ttttggtgaa tataggttct ttgctctttt tgggttcatt aacgaccaca
     8941 ggttgctgcg aagggtcagt ttttataggg gtgccaccat cgaattggta cagttggtat
     9001 ctttcaatga cacctatgag tttttgaggg tagtcagaag cagtggcata tcctgctttc
     9061 ttcagaccat aagcccaggc tttgtaatcc tttgggtcaa ggttgaacaa tgactggtag
     9121 ggtccgtttg gtctgcggat aaattcggaa tgatcaatga aggattcttc agcattatta
     9181 tattttctga agcaactt
//
LOCUS       DAOQQY010000087         9121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2865_length_9121_cov_5.361350, whole genome shotgun sequence.
ACCESSION   DAOQQY010000087 DAOQQY010000000
VERSION     DAOQQY010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9121
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2865_length_9121_cov_5.361350"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PLE23_05785"
     CDS             complement(<1..376)
                     /locus_tag="PLE23_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX82811.1"
                     /translation="MIRSLNIKNFAIIDEVSLAFEGGLTIITGETGAGKSILLGALNL
                     ILGGRVDSKMFFNQDDKCVIEAEFDISDYDLYGFFEEEDLDFDQHVIIRREILPSGKS
                     RAFINDTPVNLKALQHLSSQLID"
     gene            597..1298
                     /locus_tag="PLE23_05790"
     CDS             597..1298
                     /locus_tag="PLE23_05790"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HMX82812.1"
                     /translation="MRIVFVAIISVLFLSSCFNNIKEGEGDPQKFQRTITDFTKIKLN
                     IPAELYVIQGAEFSCTIETHPNLENYFNTDVKDQELTISSKGNFTSQKLDIYITMPFI
                     EQLSLSSSGNIYCKNEISNKDLNLNIDGSGNITMNKIVIRNLKGTINGSGNIKLVSGN
                     AMDAVYSISGSGGIEADNIDCKSLKADISGSGNITCYVAEDLDVSISGSGSLTYKGQP
                     KVKEKVTGSGKVSSF"
     gene            1333..3159
                     /locus_tag="PLE23_05795"
     CDS             1333..3159
                     /locus_tag="PLE23_05795"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HMX82813.1"
                     /translation="MKKLTKILSILMFIVSSSVFGQSSFKLITVATGYTRPLDLTHAG
                     DDRLFIVSQAGLINIIDAAGNQLPIPFLDIKDRVVDTGNERGLLGLAFHPDYKNNGYF
                     YVNYVGNDGNTRIARYTVTTDPNVADPNSEMIMMNVTQPYQNHNGGCMKFGPDGYLYI
                     GLGDGGSGGDPKANAQNTLQLLGKMLRIDVDGGIPYGIPADNPFFGKTDTLPEIWALG
                     LRNPWRYSFDKLTGDLWIGDVGQGDLEEIDVEPAGTKGGLNYGWRCYEGNNEFNLKDC
                     GPKSSYVFPVHEYDHSAGNCSLTGGFVYRGSEFPGLYGKYIYADYCSGNVYVLYKNNQ
                     GTYTNVLVAGLGNYDFSSFGEDIHGELYITGLSSGKIFKLTLDCPVAGTEYSIVRPCP
                     NTAEGGIKLDLNGGYSFNWNTGDTTQNLQNLSPGIYSVTIIDNSNFCAEVIDSILVEE
                     WLTPNLGLVWNDTLLTINAGTEAENIQWFFNGAPIPGANDVSYTPAKSGTYSASFNWV
                     ESGCAHVSDSLQIIITSNKNISSQMVNFVWPNPAKDMLNIHFNHKPDLFHYNIFTTTG
                     IRIQTGGGNENNMIDIGSLISGLYFIEINTGQERQILNFVRK"
     gene            3196..3270
                     /locus_tag="PLE23_05800"
     tRNA            3196..3270
                     /locus_tag="PLE23_05800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3231..3233,aa:Val,seq:gac)
     gene            3519..6404
                     /locus_tag="PLE23_05805"
     CDS             3519..6404
                     /locus_tag="PLE23_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMX82814.1"
                     /translation="MLEQILAKAETRLENGMYEQNITELSALLDECIKNGDEWRTMLH
                     PIFTKLVASYGNNGQLEEVNSTLKKQFGWAKKYGGSALDLGKLFHNYGQVCLMEKNFS
                     KAVHAMKIALIFKKRSLPFDLKTTIITYRELAFAYYKFYNYPLAGKYFETALALTKKL
                     PKQDAELESSIINKYTSLLLANGELETIKSLLEYSKGLPYPNKFQETDALLYGNLQMI
                     DHVYLPTNNFQAVKEIIQSSRKILDLYPDFKPAYANLFTINEAIVAYEEKNYPDALRL
                     FQSIIDPDREDLLYTELDFAIDNCLYMMYESALQTGNIPAAEKYLNAYKKIQEIDLTK
                     NPFIKLQNELQDIYFRTRQKPDVENNNHTLPLLEKFHIDIHRPVVLKDLIQTPNLEEI
                     LTVVTNILMTNVKSAQNISTANNIIDLTKRRLEFRNLKQSIGLSGSITIGSYSIEHQL
                     IAQALDMCSYLFDKTNDAKYAKDALQLMERDKNNQLYLAVKRSSLTAGKDIPQHIIDL
                     ENKVSIQVRQFLAQNTAGTSHNQDIIEIQQNYRRLIDGIIKDYPEYFIEKYKNDFFSG
                     TNTFLNKALKDYSFLEYFTTDTTIFAIAGNSGGTLHFSSTKLENPNGFREVLRDFSII
                     CANKPTADSKEDIEKFSALSETISNLIWKPSEKYLSKKLIIVPDNTLSSLPFDIILNK
                     PAAKSDNWKTLHYIMKEYHLSLAPSLQFLHLKSPESHKTKKSFLGIAYSPPQPNELHI
                     SSLNFAQKEVTEIAKQTNGETLENERSTESNITKMAAEYHVLHLACHAKADSSNGNAS
                     FLVLSSDTGNIPKIVFANNFYSSRLNNDLVVLSACESALGQAYTGEGIVGLTRSFFYT
                     GARSVLSTLWQVSDFQAGEILGEFYKNISDDKDKATSLAEAKQSYLKESGIKFSHPFY
                     WASFVVSGDPQGISLQSDDNTLAILGVIIFVFFVGLIINRRLFKKFH"
     gene            6535..7443
                     /locus_tag="PLE23_05810"
     CDS             6535..7443
                     /locus_tag="PLE23_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82815.1"
                     /translation="MLIAQDVRHDLVAYQGYRIELTFPDAQNEGNDIHTTIINSGKYP
                     VTLGIKGEKYANLQVLLEDNPAFVTNPELKRTLIRSALSKNISLAPGQQQKNIILYSK
                     PGTEGTSPPAEYKNPENLTLGKPAKKIKKEKTPKVKKEKPAQKENPVKEVKEDLTANI
                     LPASNTFDTVNCADLVISDARIIKQSKHTIKLECTITNKGIQPAPLHHYKNTNNEDIS
                     LAAYFSASGKMTRGSVLAGGHVIKNGLDKTSGYLTPGESHTVKFDISLENSSRFLNSL
                     IISIDSRQLLYECAENNNTFVIRLRD"
     gene            complement(7490..8965)
                     /locus_tag="PLE23_05815"
     CDS             complement(7490..8965)
                     /locus_tag="PLE23_05815"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMX82816.1"
                     /translation="METTKNKIRQETETSLQYWREDEKKALELLQIVGELRFDRSIEL
                     VIFRRGIYDCRPSEVLNNHLYAANYVNAPITIDTTLSIAKTILNHKEVNPCILDIGKM
                     AVNWLKNNSNNQNLENFVNDSLLPYTSSNNFSINARDVILYGFGRIGRLLARRLIEQT
                     GRGEQLRLKAIVLRPQMKDYYSEAKKRAALLMTDSVHGDFRGTITIAEDGKSLTVNGN
                     KILLIYAKDPADIDYTEYGLKDALVIDNTGVWRDGEALKAHLRPGVEHVMLTAPGKLM
                     PNIVYGVNHETLDLENTKIFCAASCTTNAISPILKVMNESLGIEKGHIETVHAYTSDQ
                     NLLDNFHKKPRRGRSAPLNMVLTSTGAAEAIESVMPELKGKFTGNAVRVPTPNVSLAI
                     MSLSLNTPTTKAALNQILKDASLHGPLVEQIQYSDSEEYVSTDAVGMTSACVLDAPST
                     IVSADGKNVIIYAWYDNEYGYTCQVVRVAKHAAQVRRPAYF"
BASE COUNT         2899 a         1914 c         1751 g         2557 t
ORIGIN      
        1 tatcaatgag ctgagaagac agatgctgaa gagctttcag attgacaggt gtatcattaa
       61 taaaagccct tgatttaccg gaaggcagta tttctctgcg aatgatgacg tgttggtcga
      121 aatcgagatc ttcttcttca aaaaaaccat ataaatcata gtccgaaata tcaaattcgg
      181 cttcaataac acatttatca tcttggttaa aaaacatttt cgaatcgacc cggccaccga
      241 gtatgaggtt tagtgcgcct aacagtattg atttaccggc acctgtttca cctgtaatga
      301 tggtaagccc tccttcaaat gcgagactca cttcatctat gatggcaaaa ttcttaatat
      361 tcagacttct tatcatattc aaaatgcaga agtgcaaagg tataacgaat atacattaat
      421 tgtataaaat ttcatcctgt atgcaataag atgagtatgg tcgaatagac aattttggtc
      481 acacaaacct gttataaaac attaccacac agaaaagaca tagaaaataa tttacatttt
      541 gaactaaatc atttacctta gatgaattat ttttgtcaat aaaccatcca ttcatcatga
      601 gaatagtatt tgtagcaatt atttcagtct tgttccttag ttcgtgtttc aacaatatca
      661 aagaaggtga aggtgatcca cagaaatttc agcggacaat tacagatttt acaaaaataa
      721 aactaaatat ccctgccgag ctgtatgtta tacaaggtgc agaattctcg tgtaccatcg
      781 aaacacaccc taaccttgaa aattatttta acacagatgt caaagaccag gaactcacca
      841 tttcatcaaa aggaaatttt acttcacaaa agctggacat ctatataacc atgccattca
      901 ttgagcaact ttctttatcg agttcgggca acatttactg caaaaacgaa atctcaaata
      961 aagacctgaa ccttaacatc gatggtagcg gcaacatcac tatgaataaa atagtgatca
     1021 gaaacttaaa aggcactatc aatggctcag ggaatatcaa acttgtcagt ggcaacgcaa
     1081 tggatgcagt gtactccatt agtggaagcg gaggtattga agccgacaac attgactgta
     1141 aatcgttaaa agccgatata agtggtagtg gcaacattac ctgctatgtt gctgaagatc
     1201 ttgatgtaag catcagtggc tcaggtagcc tcacatacaa aggtcagcca aaagttaaag
     1261 aaaaagttac cggctcaggc aaggtttcat cattctgaac gaatatttac cctgacaacc
     1321 aaatttgaat atatgaaaaa attgacaaaa atcttaagca tcttaatgtt cattgttagc
     1381 tctagcgttt ttgggcaaag ttcttttaaa ctcattaccg ttgctaccgg atatacacgt
     1441 ccgttagact tgacgcatgc tggagatgac agactgttta ttgtatcaca agcgggtctc
     1501 atcaatatca ttgacgctgc cggcaatcag ctaccaatac catttttgga tataaaagac
     1561 cgcgtagtag ataccggcaa cgagcgaggt ttgcttggtc ttgctttcca cccggactac
     1621 aaaaacaacg gatattttta tgtcaattac gtaggaaatg acggcaatac aaggattgca
     1681 agatatactg tgacaacaga tccgaatgta gcagacccca actcggaaat gatcatgatg
     1741 aatgtaacgc agccttacca aaaccacaat ggaggttgta tgaaattcgg ccctgatggt
     1801 tatctctaca tcgggttggg cgatggtgga tctggtggtg accctaaggc aaatgctcag
     1861 aacacattgc agttgctggg taaaatgttg cgcattgacg tagatggtgg cataccttac
     1921 ggcattcctg cagataatcc gttttttggt aaaacagata cattaccgga gatatgggca
     1981 ctcggacttc gcaacccttg gcggtacagt ttcgacaaac tcactggcga cctctggata
     2041 ggcgatgtag gccagggcga tttggaagaa atagatgtag aacctgcagg taccaaaggc
     2101 ggccttaact atggatggag atgctatgaa ggcaacaatg aatttaacct gaaagattgt
     2161 ggaccaaaga gtagctatgt tttcccggta catgaatatg accacagtgc aggcaattgc
     2221 tcgctaacgg gcggattcgt atatcgtggc agtgaatttc ccggtcttta tggcaaatac
     2281 atttacgccg attattgctc gggtaatgtc tatgtattat acaaaaacaa ccaaggcacc
     2341 tacaccaacg tgctagtagc cggactcggc aattacgatt tttcaagttt tggggaggac
     2401 atccatggtg aattgtacat taccggccta tcttcaggca aaatattcaa actcacactc
     2461 gattgcccgg ttgcaggaac cgaatacagc atcgtacggc cttgcccgaa tactgctgaa
     2521 ggaggaatca aacttgacct caacggtggc tactcgttca actggaacac cggcgacaca
     2581 acccagaacc ttcaaaacct tagccctggt atatattctg taactattat cgataacagc
     2641 aatttttgtg cagaagttat tgattcaata ttggtagaag aatggcttac acctaatctc
     2701 ggcctggtat ggaatgacac gctccttacc atcaacgccg gcaccgaagc tgaaaacatt
     2761 cagtggttct tcaacggtgc accgataccg ggtgcaaatg atgtcagcta cacacctgcc
     2821 aagtccggaa catattccgc aagttttaat tgggtagaaa gcggctgtgc tcatgtaagc
     2881 gactcattgc aaatcatcat cacatccaat aaaaatatca gctcccaaat ggtcaatttc
     2941 gtttggccta atccggctaa agatatgtta aacatacatt tcaaccataa accggattta
     3001 tttcattata acatattcac gacaacaggc atacgcatac agactggagg tggaaatgaa
     3061 aacaacatga tagatattgg ctcactcatc tcaggtctgt atttcataga aataaacacc
     3121 ggacaagaaa gacaaatttt aaattttgtg aggaaataaa aagaaatatc tatttttgtc
     3181 gcgcatttgc gcaatgggta cttagctcag ttggtttaga gcgccgcctt gacagggcgg
     3241 aggtcacagg ttcgaatcct gtagtaccca ctccctaatc agtttctgca cttcattatt
     3301 attgccattt gttttgttaa ataattgccc atttcgagtt attttagctt aaatactttg
     3361 tattttgctg agtatttcag atttttaaga cggcaaacac ttctgatatg caacgggcat
     3421 ttaaagtgat agttttttcc cttattttca tctgtatcag ttgtaaatac caatgtgttt
     3481 ttgcttatac gtcacgctca actagtgaca aaaaacttat gcttgaacaa atacttgcta
     3541 aagcagagac acggcttgag aacgggatgt acgagcaaaa catcacagaa ctatctgcct
     3601 tattagatga atgtataaaa aacggtgacg aatggcgcac aatgctccac ccgattttta
     3661 ccaaactagt agcttcttat ggcaacaatg gccagctcga ggaagttaac tcaacactta
     3721 agaagcaatt tggctgggca aaaaaatacg gaggcagcgc acttgacctc ggcaaacttt
     3781 tccacaacta cggtcaggtc tgtctgatgg agaaaaattt ttcgaaagcc gtacatgcaa
     3841 tgaaaatcgc actgatattt aaaaaacgca gtcttccctt cgatctgaaa actaccatca
     3901 tcacctatcg cgaacttgca ttcgcttact ataaattcta taattatcct ttggccggta
     3961 aatattttga aacggctctt gccctcacaa aaaaattacc taaacaagat gccgaactcg
     4021 aaagttcaat catcaacaaa tacacctcat tgttgttagc aaacggtgag ctggagacga
     4081 tcaaaagcct gcttgaatat tcgaaaggac taccatatcc taataaattt caagaaaccg
     4141 atgctttgtt gtatggcaac ctacagatga tagaccatgt ttatttgcca acaaataatt
     4201 ttcaggctgt gaaggaaatt attcaatcat ctagaaaaat acttgattta tacccggatt
     4261 tcaaaccagc atacgccaat ctgttcacca tcaatgaagc aattgtagcg tatgaagaaa
     4321 agaactatcc ggatgcgctc agactgtttc agtcaataat tgaccctgac cgcgaagatc
     4381 tgttgtacac cgaactagat tttgccatcg acaattgcct ttacatgatg tacgaatctg
     4441 cattgcaaac aggcaacatt ccagcagcag aaaaatacct gaatgcctat aaaaaaattc
     4501 aggaaataga ccttacaaaa aatccattta tcaagctcca aaacgagctt caggatattt
     4561 attttcgcac aagacagaag cccgatgtag aaaataacaa ccatacattg ccattgcttg
     4621 aaaaatttca tatcgatata cacaggcctg ttgtactcaa agatctgata caaaccccga
     4681 atctggaaga aattctgacc gtagttacca acattctaat gacgaatgta aagtcagccc
     4741 aaaatatatc gactgccaat aatataatcg acctaacaaa aagaagactt gagtttcgaa
     4801 atcttaaaca aagtatcggc ctgagtggaa gtatcaccat cggtagttat agcattgaac
     4861 accagctcat agctcaggca cttgatatgt gttcgtactt attcgacaaa accaatgatg
     4921 ccaaatatgc aaaggacgca ctgcagctga tggaacgcga caaaaacaac cagctttatc
     4981 tcgctgttaa gcgctcttcc cttacggccg gaaaagatat tccacaacat atcatcgatt
     5041 tggagaataa ggtaagcata caggtgagac agtttttggc tcaaaacaca gccggcacaa
     5101 gccataacca agacatcatc gaaatacaac aaaattatcg aaggctcatt gatggaatca
     5161 ttaaagatta ccctgaatat ttcatcgaaa aatataaaaa cgattttttc tccggtacaa
     5221 atacatttct gaataaagct ttaaaagact attcgttttt agaatatttc acaactgata
     5281 ccaccatatt tgcaatcgcc ggtaattcag gtggcacact tcattttagc tccacaaagc
     5341 tcgaaaatcc aaatggcttt agggaagtat tacgcgattt ttcaataata tgtgccaaca
     5401 aacctacagc tgactcaaaa gaagatattg aaaaattctc agcattgtcg gaaactatca
     5461 gcaatcttat ctggaagcct tcggaaaaat atctttcaaa aaaattgata atagtacccg
     5521 ataatacgct cagtagtctt ccgtttgaca tcatactcaa taaaccggct gccaaatctg
     5581 ataactggaa aacacttcac tacatcatga aggagtatca cctaagcctg gcaccctcct
     5641 tacagttcct ccatctgaag tcaccggaat cgcataaaac gaaaaaaagt tttttgggca
     5701 tcgcctacag tccaccacag ccaaacgaac tgcatatctc ttcactcaac tttgcacaaa
     5761 aggaagtaac cgaaattgca aaacaaacaa atggagaaac cctcgaaaat gaacgctcca
     5821 ccgaaagtaa tatcaccaag atggcagcag aatatcatgt acttcatctg gcgtgccatg
     5881 ccaaagcaga ctcatcaaat ggcaacgcat cattccttgt gctgagtagt gacactggca
     5941 atattccgaa aatagtcttt gcgaacaatt tttattcgtc gagactcaac aatgatctgg
     6001 tagtacttag tgcttgcgag tctgccttgg gtcaggctta tacaggcgaa ggaatcgttg
     6061 gcctaacaag atcatttttc tatacaggtg caaggagtgt actctcaacc ttgtggcagg
     6121 tatccgattt tcaggccggt gaaatccttg gcgaattcta caaaaacatt tcagatgaca
     6181 aggacaaagc tacttctctt gcagaagcca aacaatctta tctgaaagag tcaggtatta
     6241 agttttcgca ccccttttat tgggcttctt tcgtggtttc gggtgaccca caaggcattt
     6301 cccttcaatc tgatgataat acgctggcca tcttgggcgt tattatcttc gtattctttg
     6361 ttggtttgat tattaacaga agattattta aaaaattcca ttaatctcag cagaccctat
     6421 ttaattcaaa attggcatgg ttcatgttaa ttatatatat ccattatgtt taatatgaaa
     6481 ttttgttctt caagaattct ttcagtaatc gtacttttaa atgttttttt aaaagtgctg
     6541 atagcacaag atgtaaggca cgatttggtt gcatatcagg gttatcgtat tgaactgact
     6601 tttccggatg cccaaaatga aggaaacgat attcatacca ccatcatcaa ctctggcaaa
     6661 tatccggtaa ccctcggcat taaaggagag aaatatgcca atctgcaagt attgcttgaa
     6721 gacaatccag catttgtaac gaacccggag ttgaagagaa cactcatccg gtctgctctt
     6781 agtaaaaata tttcgctggc acccggccaa caacaaaaga acatcatctt gtattcgaaa
     6841 cccggtacgg aaggaacatc acctcctgca gaatacaaaa atcctgaaaa cctcaccttg
     6901 ggcaaaccag caaaaaagat aaaaaaagaa aaaacaccaa aagttaaaaa agaaaaacct
     6961 gcacaaaagg aaaatccggt caaggaagta aaggaagatt tgacggctaa tatcctacct
     7021 gcaagcaata catttgatac agtcaattgt gctgatttgg tcatctctga tgccaggatt
     7081 atcaagcaat caaagcatac catcaagctc gaatgcacca tcaccaataa ggggatacaa
     7141 cctgcaccac tccatcatta caagaatacc aacaatgaag acatctctct tgcagcctat
     7201 ttttcagcta gtggaaaaat gacaaggggg tcggtccttg caggcggaca tgtcatcaaa
     7261 aacgggcttg acaagacatc cggttatctt accccgggtg agagccatac cgtaaaattt
     7321 gatatcagcc ttgaaaacag ctctcgcttt ctcaattcgt tgataatatc aatagactca
     7381 agacaattac tctacgaatg tgctgaaaac aacaacactt tcgtcattcg attgcgagat
     7441 tgaacaccaa gtctccgaaa ttaaaaaatt tttcttccgg gtctgtctgt tagaaatatg
     7501 ctggtcttcg tacttgagct gcatgtttgg ccacacgtac tacctgacag gtataaccat
     7561 attcattgtc gtaccaggca tagatgataa cattcttacc atcagccgac acaatggtag
     7621 agggcgcatc cagcacacag gcacttgtca tacctacagc atcggttgac acatactcct
     7681 cagagtctga gtactgtatc tgctctacta gcggaccatg cagtgaagcg tctttcaata
     7741 tctgattaag tgcagcctta gtagtaggtg tattgagcga aaggctcatg atagcaagag
     7801 agacattcgg tgttggtacc cttactgcat tgccggtgaa ttttcctttt agttcaggca
     7861 tgaccgactc tatggcttct gcagctccgg tacttgtcaa taccatatta agaggagccg
     7921 aacgacctct tcttggtttt ttatgaaaat tatccagcaa gttttgatcg ctggtataag
     7981 cgtgtacggt ttcgatatgt cctttttcaa tacccaacga ttcgttcatt acttttagaa
     8041 ttgggctaat ggcattggta gtacaagaag cggcgcagaa tattttggta ttttcaaggt
     8101 caagtgtttc gtgattgacg ccatagacga tattaggcat aagcttaccc ggggctgtaa
     8161 gcataacgtg ttctacacct ggtcgcagat gtgccttgag tgcttctcca tctctccata
     8221 cgcctgtatt gtcaataacg agagcatctt tcagaccgta ttcggtatag tcgatatcag
     8281 ccggatcttt tgcatagatg agtagtatct tattgccatt gactgtaagc gatttgccat
     8341 cttcagctat ggtgattgtt ccgcggaaat caccgtgtac cgagtcggtc atgagcaagg
     8401 cggctctttt ctttgcttcg gagtaataat ccttcatttg tggccgtaga acgatggctt
     8461 ttaggcgtag ttgttcgcca cggccggttt gttcaataag tcttcttgcc agtaagcgtc
     8521 ctatccgacc gaagccatag agaatgacgt ctcttgcatt aattgaaaaa ttattgcttg
     8581 atgtgtatgg cagcagagaa tcgtttacga aattttcaag gttttggttg ttactgttgt
     8641 tttttagcca gttaaccgcc attttaccaa tatccagaat acaaggatta acttctttgt
     8701 ggttgagaat tgtttttgct atagaaagcg ttgtatcaat ggtgatgggc gcattaacat
     8761 agttggctgc atagagatgg ttattgagca cttcacttgg gcggcagtca taaataccac
     8821 gtctgaaaat cacaagttca atagaacggt cgaaacgtag ttcaccaact atttgtaaca
     8881 attcgagtgc tttcttttca tcttccctcc aatattgcag agaagtttca gtctcttggc
     8941 gtattttgtt ttttgtagtt tccatatatt ggtaaaattt aatttactgc aaaggtcgca
     9001 aatatttctg aacagaataa gaaaaggcac atctaaaatc tcagatgtgc ctttctttat
     9061 aataatttgt atgtttctat gctcccaggt aatgtttgag tagcttactt cttgataccg
     9121 a
//
LOCUS       DAOQQY010000088         9010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2930_length_9010_cov_7.290899, whole genome shotgun sequence.
ACCESSION   DAOQQY010000088 DAOQQY010000000
VERSION     DAOQQY010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_9010_cov_7.290899"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PLE23_05820"
     CDS             <1..397
                     /locus_tag="PLE23_05820"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HMX82817.1"
                     /translation="RCYKQYFFRKRLFSQPDGVGILGHVFGNNQGNVAQAVSQQTGID
                     TGKIMNMMVMIAPLVMGMLGKAKQTTGMDAGGLVNILGSVMGGKAGSNPIMGVLTGIL
                     DKDGDGNVMDDLMGGGIGNLLGGLFGGKK"
     gene            605..1201
                     /gene="rsmG"
                     /locus_tag="PLE23_05825"
     CDS             605..1201
                     /gene="rsmG"
                     /locus_tag="PLE23_05825"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HMX82818.1"
                     /translation="MSSVQLEQFGKFCELFAEWNAQINLISRKDIDNLEVHHVLHSLA
                     IAKNTIFEPGTRILDLGTGGGFPGIPMAILLPELHFTLMDSIGKKIMVVNDLAEKLGL
                     NNVQGIHSRVEDHKAKYDFVVTRAVADISKLLPWTRKVISGTHRNGIPNGLLALKGAT
                     YLDEIKNLPQKIYYEATPVSEYFDEEYFKEKFIVYYQA"
     gene            1205..2893
                     /gene="ftcD"
                     /locus_tag="PLE23_05830"
     CDS             1205..2893
                     /gene="ftcD"
                     /locus_tag="PLE23_05830"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HMX82819.1"
                     /translation="MKKTQIIECVPNFSEGVNQDVINQIAAQVSTVEGVKLLDVDPGK
                     ATNRTVVTMVGEPSAVIEAAFRCIKKAAELIDMSKHKGEHPRMGATDVCPLIPISGIT
                     MEETAEYAHKLAKRVGEELDIPVYMYEAAATSPERSNLSVIRAGEYEGFAEKIKLPEW
                     KPDYGPAKLNVRAGATVIGARKILVAYNVNLNTTSERRANSVAFDVREQGRIKRENDD
                     PAGAIIKDETGEPLRIPGKCKAVKGIGWYIPEYGLAQVSMNLTDTDVTPMHIAFEACV
                     DSAYQRGMRVTGSEVVGMLPKKALIDAGKYFLEKQERSTGISEEEIIHIAVKSMGLDE
                     LGPFDPKKKIIEYCMQDEGANPLIGMTLTKFAEETASESVAPGGGSVSAYVGALGISL
                     GTMVANLSAHKRGWDARWHEFSMWADRGQDIKDRLLALVDEDTRSFNAIITAMRMPKS
                     NDTEKKIRSAAIQEATKYAIQVPFAVMKTSMEAYGVIEEMVREGNPSSVTDGAVGALC
                     VNTAVQGAWMNALVNMSGLKDEEFKKNILEEGKKMAEKSTELSKEIANIVLQKL"
     gene            2900..4111
                     /locus_tag="PLE23_05835"
     CDS             2900..4111
                     /locus_tag="PLE23_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82820.1"
                     /translation="MKYRFILLLSLLCIMGATAQNSFSYMSDRKFYSPDDLIGYNFKP
                     FRLEIKDDREENLSPGVYSFGITQANLYVSGKDIEGVYSINNINTTNYGFQLLLMNAR
                     NPTLQGHLKIIVNKAKQVEAIVFKRSAKEKEIIFHIARLNETERDKEMAYFTDRGEII
                     INEPDSLYGKTIFPMTAVFYESGTQDKFAYTDSVFVKFEKKTTVIEKIIKEKKKKPEK
                     VKPEKPKKEKAKKVKKEKEKKQKDDVEEEEVKKDGLNEVIESDTEQTEESAEEAKEKI
                     KEEETTDETEETEEDKEEPKAEAVEINDDEEEVAPEEPKKKITIIEENFATVRFLARY
                     KDGREEKEEQVFKINKLVQKEDKSPEANDEKFQITVVPVSGAPFQVYLNADKSVNSVE
                     FKDRKLFVRGN"
     gene            4215..5393
                     /locus_tag="PLE23_05840"
     CDS             4215..5393
                     /locus_tag="PLE23_05840"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMX82821.1"
                     /translation="MKTVYILSAVRTPIGSFGGMFSGVSAVELGSVAIKGALDKSGVD
                     PASVQEVYFGNVVSANLGQAPARQAAKGAGLPDSVQCTTVNKVCASGMKSVMFGAQSI
                     MLGYADVVVAGGMESMTNIPYYVPKARFGYKYGNAEFIDGLHNDGLMDAYDRGAMGVY
                     ADATAAKYDITREQQDAFAIESYTRVAKATDTGKFNNEIVPVSIPQRKGDPVLNSTDE
                     EYKKVDFSKVAGLRPAFTKDGTVTAANASTINDGAAALVLASEEYVKAHNLKPVARIV
                     SFADAEQDPKWFTTTPTVAAPIALQRAGLKVSDVSNFEVNEAFAVVTLAFNKLLDVDP
                     AACNVYGGAVAMGHPLGASGARIITTLNSVLQQESGRYGLAAICNGGGGASSIIIEKV
                     "
     gene            5790..7661
                     /locus_tag="PLE23_05845"
     CDS             5790..7661
                     /locus_tag="PLE23_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMX82822.1"
                     /translation="MHILYKQLNQSDGLANNSLLTITQDDKGYIWVVTQYDGIQRYDG
                     VRFQRFGQQFVDTISHFSKVRNLNFEHGQLIAQVDDSYYRFNYADGKFYQITVGADKK
                     YSFVNNEGTRYTLTPVYVKLEYIQTPERNAVLKLPSPNAGSRSYVAYDQYDGKYWVAY
                     YGQLVSLDIKSGKAVLVSQDNYPELYELFDKMGRHGFSNIYCDSDRNLWLNNWVGVLA
                     RYNLNTRKYYIYSMETGRRTNMLRNYNAGVTAFFEDNHHQVWIGSLGAGLMKYDKEED
                     LFRFFASDGKATEDIFNSEIRAFYQDKHENIWLGTSNGIRIFNPYRAEITVLNVRIKD
                     FEQAITRNNSIEYAVQLKNGDIWVATWGNGVIVYDAELRIKKHYLFDDETSNMVWTII
                     EDKDDMVWLGCQAGNIRRFNKKTQRFEAPTKILNNISTIHGTVMDEEGNIYFGMNNGS
                     IAKWDRAHKKFSLKRNGEKNQGVSRMITSIKLDSNQKLWARTQRYLYCFDNKTLVCKY
                     SIPLVPLHLTIYKVGIEQGMTQYNDSLFVLASYFGKFGIFNVKSKNFAPLTLKENLAL
                     ARILEVRKDGIGNIWFTTSNALYRLNPQHQLNNFVADKGLITSSFSKSSSSVMSDGR"
     gene            7662..8405
                     /locus_tag="PLE23_05850"
     CDS             7662..8405
                     /locus_tag="PLE23_05850"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMX82823.1"
                     /translation="MFYTNSDILIFDPLAFDTIKAQPNIRITNLRIFEKELAIDSFFE
                     NKHTLNLNYRQNYISFDFTPMAYLPLFAQRYEYKLTGINQDWRQVDNNFSITYTKLEP
                     GNYTFMVRPEGVDDEVKIAELSFVITPPFWRSWWFITILSILAATSATIITRNRLNTI
                     RRQSDMKRKILETEMAALRAQMNPHFIFNCLTAIDHLIQNDEKDKATSYLSYFAKLIR
                     NVLDSTKNNTIPFYKDYDTIRLFIELEKF"
     gene            8433..8678
                     /locus_tag="PLE23_05855"
     CDS             8433..8678
                     /locus_tag="PLE23_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82824.1"
                     /translation="MADDELLHGDYKVPPMLVQPFIENAIHHGLRNKIDGEKTLGVRI
                     KLKNDYIIYTITDSGVGREKAAQLKQVNRPEHVSYGI"
BASE COUNT         2949 a         1624 c         2068 g         2369 t
ORIGIN      
        1 aagatgctac aagcaatatt tttttagaaa acgacttttt tcacagcctg acggtgttgg
       61 tattcttggt cacgtgttcg gtaataacca gggcaatgtg gctcaggccg tttcgcagca
      121 aacaggcatt gatactggta aaatcatgaa tatgatggta atgattgccc cactggtgat
      181 gggtatgctt ggaaaagcaa agcagactac cggtatggat gcaggtggcc ttgtaaatat
      241 ccttggaagc gttatgggtg gaaaggcagg cagtaacccg atcatgggtg tgcttaccgg
      301 cattcttgac aaggatggtg atggtaacgt aatggacgac ctgatgggcg gtggcatcgg
      361 caacctactc ggtggtttgt tcggaggcaa gaagtaattc ttctttatta aaaaatatta
      421 atcgggagat gaagtttggt tttcatctcc tgtttttttt gatatgatag taaaagcgta
      481 ttttttacaa gaaaacaccc ggttttacat ataatgataa gaatctgaca gaatattctt
      541 ccaatctttg tacctttgta tgccagtaac agctatggag gttttgaaaa aatattttcc
      601 cgatttgagt tcagttcagc ttgaacaatt tgggaaattc tgcgaattat ttgcggagtg
      661 gaatgcccag attaatctta tttcaagaaa agatatagac aacctcgaag tacaccatgt
      721 actgcacagt cttgcaatag caaaaaatac aatattcgaa ccaggtaccc gaatactcga
      781 tcttggaaca ggaggaggat ttcccggcat tccgatggct attcttctgc ctgagttaca
      841 tttcacgcta atggatagca ttggcaagaa aataatggtg gtcaatgacc ttgccgaaaa
      901 acttggtctc aacaatgtac aaggcatcca ctcacgtgta gaagatcata aggcaaaata
      961 cgattttgta gtaacgcgtg cagtagctga catatcaaag ttactgccct ggacacgcaa
     1021 ggtgatatcg ggcacacacc gcaatggcat ccccaatgga ttacttgcat taaaaggtgc
     1081 cacttacctt gacgaaatca aaaaccttcc tcaaaaaata tactatgaag ctactccggt
     1141 gtcagaatat tttgatgaag aatattttaa agagaaattt atcgtttact atcaagcata
     1201 aaatatgaaa aagactcaga taatagaatg tgttcctaat ttctccgaag gagtaaatca
     1261 ggatgtcatc aatcagatag ccgctcaggt atcaacagta gaaggtgtca aactactaga
     1321 tgttgatccg ggcaaagcta ctaaccgaac cgtcgtgacc atggtaggcg agccatctgc
     1381 tgttattgaa gcagcattta ggtgtattaa aaaagcagca gaactcatag acatgagtaa
     1441 gcacaaaggc gaacacccac gtatgggcgc aacagatgtt tgcccactaa taccgatatc
     1501 gggcattaca atggaagaaa cagcagaata tgcacacaaa ctggctaaac gagtgggaga
     1561 ggagctggat atacctgtat atatgtatga agctgcagcc actagccccg aacgatcaaa
     1621 tctctcagta attcgtgccg gtgagtatga aggattcgca gaaaagataa aactacctga
     1681 atggaaaccc gactacggtc ctgcaaagtt gaatgtacgt gcaggtgcta cggttatcgg
     1741 tgccagaaag attcttgtag cctataatgt caatctcaat actacatcag agcggagagc
     1801 caactcggta gcattcgatg tgcgcgaaca aggtcgtatc aagcgcgaaa acgacgatcc
     1861 ggcaggtgct attataaaag atgagacagg cgaaccgctg cgaatacccg gcaagtgcaa
     1921 ggctgtgaaa ggcattggct ggtatattcc tgaatacggt ctggcacaag tatccatgaa
     1981 ccttacagat acagatgtta ccccgatgca catcgcattc gaagcctgtg tggacagtgc
     2041 ttaccagcgg ggtatgcgtg taacaggatc ggaagtagta ggtatgctgc caaagaaggc
     2101 tttgatcgat gcaggcaagt attttcttga aaagcaggag cgctcaacag gtatttcgga
     2161 agaagaaatc attcacatag ctgtaaaatc aatgggcctt gacgaacttg gtccatttga
     2221 cccgaagaaa aaaatcatcg aatactgcat gcaagatgaa ggtgccaatc cgctcattgg
     2281 tatgacactt accaaatttg ctgaagagac agccagtgaa agcgtagccc cgggtggtgg
     2341 cagtgtgtct gcttatgttg gtgcattggg tatctcgctt ggtacgatgg tagctaatct
     2401 gtcagctcat aaacgcggat gggatgcccg ctggcatgag ttctcgatgt gggcggatag
     2461 agggcaggat atcaaagatc gattgctggc actagtggac gaagacactc gctcattcaa
     2521 tgccatcatt actgctatgc ggatgccaaa atcaaatgat actgaaaaga aaatacgcag
     2581 tgctgccatt caggaagcta ccaaatatgc catacaggta ccttttgcgg tgatgaagac
     2641 gtctatggaa gcgtatggcg taatagagga aatggtaaga gaagggaacc cgagttcggt
     2701 gacagatggc gctgtgggtg cattgtgtgt caataccgct gtacaaggag cctggatgaa
     2761 tgcactcgta aacatgtcgg ggctcaaaga cgaagagttt aagaagaata ttcttgaaga
     2821 agggaagaaa atggcagaaa aatcgacaga attgtcaaag gaaattgcca atattgtatt
     2881 acaaaaatta tagcgcttga tgaaataccg ttttattctg cttctttcgt tgctgtgcat
     2941 tatgggtgcg acagcacaga attcgttcag ctacatgtct gatagaaagt tttattcgcc
     3001 ggatgacctg attggatata attttaagcc attcaggctg gaaataaaag acgacagaga
     3061 agagaatctc agccctggtg tttattcatt tggtataaca caagccaacc tctatgtgag
     3121 tggcaaggac atagaaggcg tgtacagcat caataatatc aacaccacca attatgggtt
     3181 tcagcttttg cttatgaatg cccgcaaccc gactctacaa gggcatttga agataatagt
     3241 caataaggca aaacaagtgg aagccattgt attcaaacgt tcggctaaag agaaagaaat
     3301 cattttccat attgccaggc tgaatgaaac cgaacgcgac aaagaaatgg cttattttac
     3361 agaccgcggt gaaataatca tcaacgaacc ggactcgctg tatggcaaga ctatttttcc
     3421 gatgactgct gtattttacg aaagcggcac tcaggacaaa tttgcctata ccgacagtgt
     3481 ttttgttaaa tttgaaaaaa agacaactgt cattgaaaaa atcataaaag aaaaaaagaa
     3541 gaaaccggag aaagtgaagc cggaaaaacc aaaaaaggaa aaagctaaaa aagtaaagaa
     3601 agaaaaggag aaaaagcaaa aagatgatgt tgaagaagag gaagttaaaa aagacggctt
     3661 gaatgaagta atagaaagtg ataccgaaca aacggaagaa tcggcagaag aagcaaaaga
     3721 aaaaataaag gaagaagaaa caacagacga gaccgaagaa accgaggagg acaaggagga
     3781 acccaaagca gaagcagtag aaataaacga tgacgaagaa gaagtcgcac cggaggagcc
     3841 gaagaagaaa attacaatca ttgaagaaaa ttttgctaca gtaagatttc tggcgaggta
     3901 taaggatggt agggaagaaa aagaagaaca ggtgtttaaa ataaacaaac tggtgcagaa
     3961 agaagacaag agcccggaag caaacgatga aaaattccag ataacggtag tgcccgtatc
     4021 aggtgcacca tttcaggtat atctgaatgc tgataaatct gttaattcgg ttgagttcaa
     4081 ggacagaaaa ttatttgttc ggggcaatta atgaattatg cagagattga ctcgtatgaa
     4141 tgcattattg aaaactttaa caaaaaatag ttgttttaaa ttattcagaa aaataaataa
     4201 acaaaattat tgatatgaaa acagtgtata ttctatcagc agttcgcaca cccattggtt
     4261 cattcggcgg aatgttttca ggtgtgtcgg ctgtggagct tggttcggta gccatcaaag
     4321 gagccttaga taaatcaggt gttgacccgg ccagtgtgca ggaggtgtat tttggtaatg
     4381 tagtgtctgc caatcttggt caggcgcctg ctcgtcaggc tgccaaaggt gccggattgc
     4441 cggattctgt acagtgtacg accgttaata aagtgtgcgc atctggtatg aaatcggtga
     4501 tgttcggtgc gcaaagcatc atgcttggct atgccgatgt agtagttgcc ggaggaatgg
     4561 aaagcatgac caacatcccg tattatgtac caaaagccag attcggctac aaatacggca
     4621 atgcagagtt tatagacggt ctgcacaatg acggcttgat ggatgcttac gaccgtggag
     4681 ctatgggtgt atatgccgat gcaacagcag ccaaatacga tataacacgt gaacaacagg
     4741 atgctttcgc tatcgaatca tatacaagag tagccaaagc aacggataca ggaaaattca
     4801 acaacgaaat agtgccggtg agcattccgc aacgcaaggg cgatccagta ctcaactcca
     4861 ccgatgaaga atacaaaaag gttgattttt caaaggtggc cggattgaga ccggcattta
     4921 ccaaagacgg tacggtcaca gcagctaatg cttctaccat caatgacggt gcagctgccc
     4981 tcgtactggc tagcgaagaa tatgtaaaag cccataattt gaagcctgtg gcacgaattg
     5041 tcagttttgc agatgcagag caggacccaa aatggtttac aaccacaccg actgttgcag
     5101 caccgattgc actacaaaga gcaggtctaa aggtatcgga tgtaagcaat tttgaagtaa
     5161 atgaagcatt tgcagtagta acattggcct tcaacaaatt gctggatgtt gaccccgctg
     5221 cttgcaacgt gtatggtggt gctgtagcta tggggcatcc gcttggtgct tcaggggcac
     5281 gtatcattac tacgctcaac agcgtccttc agcaagaaag tggccgctac ggtctggctg
     5341 ccatttgtaa tggtggaggt ggtgcatcat ccattatcat cgaaaaggtg taaagcctga
     5401 tatgatagat taagtaaaag tccttcagta ttgttctgag ggactttttt gtttttttag
     5461 taaaatttgt gtgaaattcg aatttctccc caagctgctt gttaataaaa tatgtgggtt
     5521 cagttaaata taaaaagaac ccagttatcc ggaaaacctt tccacttaat aaaaaattgt
     5581 aaaaatttgt caaaacatga atatatttat gtgctacaat gggctatgat ggatatgagg
     5641 taatgaatgg cgtttagctc gtttacctgg aaatctgatt tttattgaaa tgcttgctta
     5701 tttatacaaa agcgaagttg aaaaaatcca tcttcatatt atcattcatc attttgtcgg
     5761 gaaccatttt cggacaaaaa gctgaatttt tgcatattct ttacaaacaa ctcaatcagt
     5821 cggatgggct ggcaaataac agtcttttga ccattacaca ggacgacaaa ggatatatat
     5881 gggtagtgac tcaatatgat ggcattcaaa ggtatgatgg tgtaagattt cagcgattcg
     5941 gccagcagtt tgttgataca atatcgcatt tttcaaaggt gcgtaatctt aattttgagc
     6001 atggacaact aatcgctcag gtagatgatt catattacag atttaattat gctgacggga
     6061 aattttatca gataacagtt ggtgctgata aaaaatattc atttgtcaat aatgaaggta
     6121 caagatatac attaacaccg gtttatgtaa aactagaata cattcagaca cctgagagga
     6181 atgctgtttt gaaattgcct tcgcctaatg caggctcgag atcctatgtt gcatacgacc
     6241 agtacgatgg taagtattgg gtggcttact atggtcagtt ggtgtcactt gatattaagt
     6301 cgggtaaggc cgtcctggtt agtcaagaca attatccgga attgtatgag ctttttgata
     6361 agatgggtag gcatggattt tcaaatattt attgcgattc ggacaggaat ctttggctca
     6421 ataactgggt tggtgtgctt gccagataca atctcaatac gcggaagtat tatatttatt
     6481 cgatggagac tggtcgtagg accaacatgc tccgaaatta taatgcaggt gttacagcat
     6541 tttttgaaga caaccatcat caggtttgga taggttcgtt gggtgcggga ttgatgaagt
     6601 atgataaaga ggaagatttg tttcggttct ttgcttcaga cggcaaagcc acagaggata
     6661 tttttaatag tgagatacgt gcattttatc aggacaaaca tgagaatatt tggcttggca
     6721 catccaatgg tatcaggata tttaatcctt acagagcaga aataacagta ctgaatgtgc
     6781 ggataaagga ttttgaacag gctataacac ggaataactc cattgaatat gctgtacagt
     6841 taaagaatgg tgacatctgg gttgccacct ggggcaatgg tgtcatcgtc tatgatgcag
     6901 aattgcggat taaaaaacat tatctatttg atgatgagac gtcgaatatg gtttggacaa
     6961 tcatcgaaga taaggacgat atggtatggc ttgggtgtca ggcaggcaat atcaggcgct
     7021 tcaacaaaaa aacacaacgc tttgaagcac caacgaaaat attaaacaat atcagtacca
     7081 tacacggtac tgtaatggat gaagagggga atatttactt tggtatgaac aatggaagta
     7141 tcgcaaaatg ggatagagcg cacaaaaaat tttctctgaa acgaaatgga gaaaaaaatc
     7201 agggtgtaag ccgtatgata acatccatca aactcgatag taatcaaaaa ctttgggcaa
     7261 gaacgcagcg ctatttgtac tgttttgaca ataagacctt ggtttgtaag tattctatcc
     7321 cattagtacc gttgcatcta acaatttata aagtgggtat tgaacaaggc atgactcaat
     7381 ataatgatag tctttttgtt ttggcatcgt attttgggaa attcggcatt ttcaatgtaa
     7441 agtcaaagaa ctttgcgcca ttgaccttga aggaaaatct ggcattagcc aggattctgg
     7501 aagtaaggaa ggatggtata ggtaatattt ggtttacgac aagtaatgca ctatacagat
     7561 taaatcctca gcatcaactc aataattttg tagcagacaa ggggcttatt acttcatcct
     7621 tcagtaagag tagctcctca gtcatgtcag acggacgata aatgttctat acaaactcag
     7681 atatattgat attcgatccg ctggcttttg atacaataaa agcacaaccc aatattcgga
     7741 ttaccaattt gagaatattt gagaaagaat tggccatcga ttcttttttc gaaaacaaac
     7801 atacactcaa tctcaattac agacagaatt atatatcatt tgattttaca ccaatggcct
     7861 acctgcctct attcgcacaa aggtatgagt ataagttgac aggcattaac caagactggc
     7921 ggcaggttga caataatttt agcatcactt acacaaaact tgaacccggc aactatactt
     7981 tcatggtaag gccagaaggc gtggatgatg aagtgaagat tgctgagcta tcatttgtca
     8041 tcacacctcc attttggcgt agctggtggt ttattactat tttatcaata ttggccgcta
     8101 caagtgctac cattattaca agaaaccggc tgaataccat tcgccgacag tctgatatga
     8161 aaaggaagat actcgaaacc gaaatggcag ccttgcgtgc acagatgaac ccacatttta
     8221 tctttaattg cctcacagct atcgaccatc tcatacaaaa tgacgaaaaa gataaggcga
     8281 cgagttatct gagttatttt gcgaagctga taaggaatgt gcttgacagt accaagaaca
     8341 ataccatacc attttataaa gattatgaca ccatccggct gtttattgag cttgaaaaat
     8401 tttgaagtag caacaatttt tcataccacc tggtggcgga tgacgaactg ctacacggag
     8461 attataaggt gccacctatg cttgtccagc catttataga aaacgcaata catcatggct
     8521 tgcgcaataa aatagacgga gaaaagacat tgggcgtacg gataaaatta aagaatgatt
     8581 atattattta tacaataaca gatagtggtg ttggtagaga aaaggcagca caactcaaac
     8641 aagtcaaccg gcccgaacat gtgtcctacg gtatctagat ttcgaaggaa cggcttatgc
     8701 agttcaattt tccaaatctg acaagcatac aaacaggaga tactgccgga ttgatcaagt
     8761 ttacagattt gtatgataac ggaaaagcgt gtggaactaa agtagaaata caccttaaaa
     8821 ttgatccaac aataaactaa tacaactatg ttaaccgcaa tacttgtaga tgatgaaata
     8881 atgagcctta actctctaag acttaaaatt tcaggcaatt tcccggagat tcaggtgctg
     8941 gcagcttgta cctctgccgg tgatggattg ggagccattg aagagatgca tcctgacatt
     9001 gtttttcttg
//
LOCUS       DAOQQY010000089         8963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2964_length_8963_cov_6.190503, whole genome shotgun sequence.
ACCESSION   DAOQQY010000089 DAOQQY010000000
VERSION     DAOQQY010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2964_length_8963_cov_6.190503"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PLE23_05860"
     CDS             <1..514
                     /locus_tag="PLE23_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HMX82825.1"
                     /translation="ADILNAIIANKNTEIKRKYLYNHWVNFDPVNTEKSYQKFMNLAK
                     QVDAAFVSGFGYGFETDRGRIWLKYGPPNDKISVEEDPNAPPYEIWVYNEFPKTKQGI
                     VKFLFYNPNLGNDYILLHSNCRTEVNNPRWLKELYKGAKSNYNADEYNETGKISDGFL
                     KHASEYFKDL"
     regulatory      607..706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            759..3188
                     /locus_tag="PLE23_05865"
     CDS             759..3188
                     /locus_tag="PLE23_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMX82826.1"
                     /translation="MFRLLVFLLLLSSSLFVNAQCKVDFYFYSNVSNTPLVAEIFSNG
                     IQLNPSNTDVRFSYKHTCGQHSVFEIRAVGYKNQQVELINRTDTMIDIFLEPISLTLP
                     DLEVLASRFPGNVLPAVSKLSGSDLQEINTGKDIPYLLESIPSVFSTSDAGNQTGYTG
                     LRIRGSDQTRINVTINGVPVNDAESHNVFWVDLPDLASSVDQIQIQRGVGTSSYGAGS
                     FGANISIITKPASLQPYMTTAFSGGSFNSFKKSVLFGTGLINKHFSFEGRWSGIDSDG
                     YIDRAYSHLSSLYGNLNYHNRKNTIRLIAFSGDERTYQSWYGVPESVLSGDVEQMNAY
                     ADRNGLSDTERQNLLGSGRTYNYYQYKDQTDNYRQSYFQLIYSSQLSDNLLFTSVAHL
                     TRGKGYYEEWKPADKLADYGLLPIVAGQDTISEANIVRKRWLDNYFYGMNAQLKYETT
                     SLFLSGGLTAYKYDGNHFGRINWIDNYPGLVPGFEYYKGKGIQDEFSFFGKLKYGLTQ
                     NLDVLADLQYRLIRYNAYGTNNDLQEFVIYRQYNFFNPKIACSYRPANGWYINASVGR
                     SNSQPFRDDFINATPGRQPKSEQLTDYELEIQKSGKNWSAIASFYYMKYKDQLILTGE
                     LNDVGAAVRTNVAESYGRGVELQLSWQPFPFLQWTGNGSFSSNIIRAFDYLLYDYDNG
                     GEVLETFVNTPISFSPSTILYSDIRLNHKKWSLDVTAKYIGQQYLDNTGSVERAIDPY
                     TVINSTLSWKTKIFSNSVLQLSGQIFNLADLKYSSNGYTYSYLYGGLVTENFYYPQAG
                     RHFMLSCKIDW"
     gene            3323..3535
                     /locus_tag="PLE23_05870"
     CDS             3323..3535
                     /locus_tag="PLE23_05870"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMX82827.1"
                     /translation="MKRNMSQTDRAVRIFVALLVFYLYYSGTLAGGVGIALMIISIVF
                     VLTSLVSFCPLYRVVGFSSCKEEEKP"
     gene            3948..5174
                     /locus_tag="PLE23_05875"
     CDS             3948..5174
                     /locus_tag="PLE23_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82828.1"
                     /translation="MKRLFSFFIMCTLFAPNTQAQCWQRMVGGVHSLAVRTNGTLWAW
                     GRNTEGQLGDGSNTDKDSSVQIGIDTNWQGVACGSFHSFAIKSDSTLWAWGRNTEGQL
                     GDGTNISKNSPVQIGSDTNWHTLAAGIFHTLAIKTDGTLWAWGLNLEGQLGDGTNIDK
                     NSPIQIGLANNWQSVTAGRSSSLAVKTDGTLWVWGDNNAGQLGNGTKSDTEESPVQIG
                     SATDWQSVAGGLGFSMAIKKDGTLWSCGTNVSGQLGDGTNVLKTSFVQIGSDTNWQNV
                     VGGNFHALALKTDETLWAWGQNAAGQLGDGTNVDRYTPQQIACPSAGTNNQLQQGMVK
                     IFPNPVSNFINIKIMDGNFENLTYEVLNILGQKVEMNSLRPDSLRIDLPPMPQGMYFL
                     TIRRGNELITKAFVKI"
     gene            5267..5833
                     /locus_tag="PLE23_05880"
     CDS             5267..5833
                     /locus_tag="PLE23_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 domain-containing protein"
                     /protein_id="HMX82829.1"
                     /translation="MTNDYLESTIKQFEYYKLLGEKTFAQLSDEQLFWQYNEESNSIA
                     TIVKHLWGNMLSRWTDFLTTDGEKEWRKRDEEFDNDIVDRVELLAKWNEGWTCLFNAI
                     NSLTENDLTKEIFIRNQGHNVTEAVNRQLAHYPYHIGQIVFIGKMFCNEKWTSLSIPR
                     GNSKEYNADKFSEPKHKQHFTDEYLNKK"
     gene            complement(6234..7892)
                     /locus_tag="PLE23_05885"
     CDS             complement(6234..7892)
                     /locus_tag="PLE23_05885"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX82830.1"
                     /translation="MKILIIGGVAGGATAATRLRRLSEENEVIIFEKGSYISFANCGL
                     PYHIGGVIHERDALLLQTPESLKERYNLDVRIHTEVLSIDRNAKTVSIKNLNTGDTYT
                     ESYDKLLLSPGAEPIRPPFEGIDSDKIYTLRNIPDMDKIVSKTRSAKSFVVVGGGFIG
                     LEVAENLLEAGKQVTLVELGNQLMAPVDFDIASFIHEKAEQHGLALLLNTGVEKFADL
                     GDTIRVFLNNGTSIDTEAVILAIGVKPETKLAKDAGLETGTTGGILVNGYMQTSDPDI
                     YAVGDAVEVEHYIHHSKVLIPLAWPANRQGRMVADNISLGNRYTYPGSLGSSILKFFE
                     LTVACTGLSEKFLKRNNIPHKTAIVTRGHHAGYYPGAKNIVLKLIFDDSGRIFGAQAV
                     GEKGIDKRIDVIATAIKGNLTIFDLPEIEITYAPPYNSAKDPVNIVGYTAENILNGIL
                     DMVNYDEFDRFVNENNAIILDVRTPREYAEGHITGAINLNVDELREALPTLDKDKTYV
                     IYCQIGLRGYLANRILRNHGFRAVNLNGGYNLWTKVHGKSKVQA"
     gene            8153..>8963
                     /locus_tag="PLE23_05890"
     CDS             8153..>8963
                     /locus_tag="PLE23_05890"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HMX82831.1"
                     /translation="MKKTAENILDVTVLEPSKKHPTIFAWFDELAGGECFIIHNDHDP
                     KPLYYQMYGERGGVFNWEYLEQGPKWWKVRITKRRHDETQQTIGQMVAQDARKAEIFR
                     KYNIAYGSDGKKILNEAAKERGLDVTKIEQELQQLDRSKYAEAAKYNELDLDFLIDYI
                     IQTHHGHVRRQIPTIREYASKVMVKHAAGHPELNKIYKLVEELYTEISTNIQKEELIL
                     FPYIKEMVAAARNNIPFAQSPFGSVQQPVNMMEMEHEAACSLLKEIRRLSLN"
BASE COUNT         2737 a         1696 c         2020 g         2510 t
ORIGIN      
        1 tgcagatatt ttgaacgcca tcattgccaa taaaaatact gaaataaagc gaaagtacct
       61 gtataatcac tgggtgaatt ttgaccctgt caatacagaa aaatcttacc agaaatttat
      121 gaatctcgct aagcaggtag atgccgcttt tgtgtccggt tttggatacg gattcgaaac
      181 agatagagga agaatctggc tgaaatatgg gccgccgaat gacaaaattt cagtagagga
      241 agaccctaat gcaccgcctt atgagatctg ggtgtacaat gaatttccta aaaccaaaca
      301 gggcatagtg aaattcttat tctacaaccc gaatcttggt aatgactata ttttattaca
      361 ctccaactgt cgtacagagg tcaataaccc acgttggctt aaggagttgt acaaaggcgc
      421 aaaaagcaat tacaacgctg atgaatacaa cgaaaccggc aaaattagcg acggatttct
      481 aaaacacgct tcagaatact tcaaggattt gtagttcgat ttagcatgac ctaaagcttt
      541 cattctgatt gtttttttaa atttcggaca cattttatcc gcttatatct ttaattgata
      601 tatctttgca gtacacaggg gtgctctgtc atttgtcaga gctgagatta tacccataga
      661 acctggacag gtaatgctgt caaggaaata tgtaggtgaa ataaaaaccg gagccaaccc
      721 cgcgctaccg gattatttca gattaaacca aatcaatcat gtttcgtctt cttgtttttt
      781 tgctgttgtt aagcagttca ttgtttgtta atgcacaatg taaagtagat ttttattttt
      841 attcaaatgt aagcaatact cctttggttg ctgaaatatt cagtaatggc atacaactta
      901 atccatctaa tactgatgtc cgctttagct ataagcatac gtgtggtcaa cattctgtat
      961 tcgaaattag agccgtagga tacaaaaatc agcaagttga gctaattaat cgtacagata
     1021 cgatgattga tatttttctt gaaccaataa gccttacact tccggatttg gaagttcttg
     1081 ccagtcgctt tccaggcaat gtattacctg ctgtgagcaa gttgtcgggc agtgaccttc
     1141 aagaaattaa taccggtaag gatattccct acctgctcga atcaatacca tcggtttttt
     1201 ctacatcaga tgccggaaat cagaccggat ataccggact acgtatccga gggtctgacc
     1261 agacacgcat caatgtaact atcaacggcg taccggtcaa tgatgcagag tcacacaatg
     1321 tattttgggt agatttgccg gatctcgctt catccgttga ccagatacaa atacaacggg
     1381 gggtaggtac ttcttcttat ggggcaggtt cttttggtgc caatatcagc attattacca
     1441 aaccggcatc cttacaacct tatatgacaa ctgctttttc aggaggcagt ttcaattcat
     1501 tcaaaaaatc agttttgttc ggtaccggac ttatcaataa acatttcagc ttcgaaggca
     1561 ggtggtccgg cattgattct gacggatata ttgatcgtgc atacagccac cttagttcat
     1621 tatatggcaa ccttaattat cataatcgta aaaatacgat aaggcttatt gctttttccg
     1681 gagatgaaag aacctaccag tcgtggtacg gtgtgccgga gagcgtattg tcgggtgatg
     1741 tagagcagat gaatgcctat gccgatagga atggactttc ggatacagaa aggcagaatt
     1801 tgcttggtag tggacgtact tacaattatt atcaatataa agaccagaca gacaattaca
     1861 gacaaagtta ttttcaactt atttattcat cgcaattgtc agacaatctg ctatttacat
     1921 cggtagcgca tctcacacga gggaagggtt attacgaaga atggaaaccg gcagacaaac
     1981 tggctgatta cggtttgctt ccaatagtgg caggtcagga tactatctca gaagccaata
     2041 tcgtacgcaa acgatggctt gataattatt tttatggaat gaatgcacag cttaaatacg
     2101 aaactacatc tttatttctg tcgggtgggc ttacagcgta taaatacgat ggaaatcatt
     2161 ttggaagaat aaattggatc gacaattatc ccggtctggt acccggattt gaatattata
     2221 aaggaaaagg aattcaggat gaatttagtt tttttggaaa attgaaatat ggcctgacac
     2281 aaaatctgga tgtgttggct gatctccagt acagattgat acggtataat gcatacggca
     2341 ccaacaacga tttgcaggaa tttgttattt acagacaata caatttcttc aatcctaaaa
     2401 tagcatgcag ttaccgacct gctaatggct ggtacatcaa tgcatcggta ggaaggtcaa
     2461 actctcagcc tttcagagac gacttcatca atgccactcc cggacgccaa ccgaaatctg
     2521 aacagttgac tgattacgaa ttagaaatac agaaatccgg taaaaactgg tctgccatag
     2581 cctcatttta ctacatgaag tacaaagacc agctcatact tacaggtgag ctcaacgacg
     2641 taggggctgc cgtacgcacc aatgttgcgg agagctacgg acgtggtgtc gagttgcagc
     2701 tgagttggca gccttttccg tttttgcaat ggacaggcaa tggaagtttt agcagcaata
     2761 ttatccgagc gtttgattat ttgttgtacg attatgataa cggtggcgaa gtcttagaaa
     2821 catttgtcaa cacaccgatt agtttttcac catctaccat attgtacagc gacatacgat
     2881 taaaccataa aaaatggtca ttggacgtga cagccaagta tatcggacag cagtacttgg
     2941 ataacacagg ttcagtcgag cgtgccattg acccatatac tgttattaac agcaccttat
     3001 catggaaaac aaagattttt tccaattcgg tgttacaatt atccggacag atttttaatc
     3061 tggctgattt gaaatattcg agcaatggtt atacttacag ttatctctat ggtggattag
     3121 taactgaaaa tttttactat ccgcaggcag gacggcactt tatgttgagc tgtaaaatag
     3181 actggtaata agctaacagt atcaggaaca tgcgatgatt tggcaatatg ttataaaaat
     3241 actggttttt atttcatcaa tcgaattgat ttcattagat ttgcttcagc agatttgcag
     3301 ctaaccaatc aaatctttag agatgaaaag aaatatgagc caaacagaca gggcagtcag
     3361 aatatttgtt gctttacttg tgttttattt atattattcg ggtactttag caggtggggt
     3421 aggcattgcc ttgatgatta tttccatcgt ctttgtactc accagtcttg ttagtttttg
     3481 tcccttgtat cgggtcgtcg gctttagttc ctgtaaggaa gaagaaaaac cgtgaaccaa
     3541 ctgttagcaa ctgtatattt gtttcagttt tgggtgaatt ttccattcac ttgtatgcgt
     3601 cttttcctaa aacaggttta cacaaaaatg tgtaaaaatg gataaaaaaa ttaaatcaat
     3661 atcaggactt aaaaaagtac tgtacactaa taacaggatg taatctataa cttgtgaact
     3721 tttattagga cgagtcaagg ttaagaattc aaaatatttg cttatttttg ttagattaag
     3781 ctgaaaatat ggtttttgga cagactgacg cacgctgaca ttcttaaaat taaaatggaa
     3841 tgaatacaaa ccacattgac agatcaacga aatttatgat taaagacttt aaaggtttta
     3901 cgacaattca tttggcttct attcatacat caacctaatt tgtttttatg aaaagattat
     3961 tttccttttt tattatgtgt acactctttg ccccaaacac ccaagctcaa tgttggcagc
     4021 gtatggtagg tggagttcat tcgcttgcag ttagaaccaa tggaaccctc tgggcttggg
     4081 ggcgcaatac agaaggacag ttgggcgatg gatccaatac cgataaagac agctcggttc
     4141 aaattgggat agacactaat tggcaaggcg ttgcgtgtgg cagttttcat tcctttgcga
     4201 ttaagtcaga cagcacactt tgggcttggg gacgcaatac agagggacag ttgggcgatg
     4261 gaaccaatat cagtaaaaac agcccagttc aaattggttc agacaccaat tggcatacgt
     4321 tggctgctgg aattttccat accctcgcga ttaaaaccga cgggacgctt tgggcatggg
     4381 gacttaattt ggaaggacag ttgggcgacg gaaccaatat cgataaaaat agtcctattc
     4441 aaatcgggtt ggcaaacaat tggcaaagtg tgacggccgg acgttctagt tcccttgcgg
     4501 ttaaaaccga cggaaccctt tgggtttggg gcgataacaa tgccggacag ttgggcaacg
     4561 gaaccaagag tgatacagaa gaaagcccag ttcaaatcgg gtcggccacc gattggcaaa
     4621 gtgtggcggg tggattagga ttttctatgg cgattaaaaa agatggcacg ttatggagtt
     4681 gtggtaccaa tgtttccggg caattaggag atggaaccaa tgtcctcaaa acaagcttcg
     4741 ttcaaattgg gtctgacacc aattggcaaa atgtagtggg tggaaatttt catgcgcttg
     4801 cgttgaaaac agacgaaaca ctttgggctt gggggcagaa tgcagccgga cagttaggcg
     4861 atggaacgaa tgtggacaga tatacaccac agcagattgc ttgccctagt gcgggtacaa
     4921 acaatcaact tcaacaaggt atggtgaaaa tttttcctaa tccagtatca aatttcatca
     4981 atataaaaat aatggacggg aattttgaaa atttgaccta tgaagttttg aatattttag
     5041 ggcaaaaagt cgagatgaat agtttgcgac ctgattcttt acgcattgat ttgccaccca
     5101 tgccgcaagg aatgtatttt ttgactatca gaagaggaaa tgaactaata acgaaagctt
     5161 ttgtcaaaat ttaatctaac gcacaatgta tagtcgccaa tgcaatcaat tggtggcaat
     5221 tataccgaac cataatacaa ctgaaattag acgatataaa aaagccatga caaacgatta
     5281 tttagaaagc acgataaaac aatttgagta ttataaattg ttaggagaga aaacctttgc
     5341 ccaactttct gacgaacaac ttttttggca atacaatgaa gaaagtaata gcatcgctac
     5401 aatcgtaaaa catttgtggg gaaatatgct ttcgcgttgg acggattttt tgacaacgga
     5461 cggagaaaaa gaatggcgaa aaagagacga ggaatttgat aacgatattg tggatagagt
     5521 cgaactgtta gcaaaatgga atgaaggttg gacttgtttg tttaatgcga taaattcgtt
     5581 gacagaaaac gatttaacaa aagaaatttt tattcggaat caaggacata acgtaacaga
     5641 agctgtaaac agacaacttg cacattatcc ataccatatt ggacaaattg tgtttatcgg
     5701 gaaaatgttt tgcaatgaga agtggacttc actatccatt cctagaggaa actcgaaaga
     5761 atataatgca gacaaatttt ctgagccaaa acataaacaa cattttacag acgagtattt
     5821 aaacaagaaa taaaaataaa attatgttaa gactatttat acctatattg ttttttgcaa
     5881 tctttatagc ttgggtttta taccgacttt tgataaagaa agacttaaaa caaaatctaa
     5941 actctttata tcttggcctg atatttatag gtgtttgggt attaatttat tacttcttgc
     6001 tgaaataggt ataataaaca ttcgaaaaat gcccccgatc ataaatgaaa aggcttttgt
     6061 aatacttgta tgtgataata gtattaccgg gcacaatgaa taactgcttg tgcaactaaa
     6121 tcgcgccagg gatggcaggc ggcacaacgt aagccgtaca gcccgacccg acagggagac
     6181 gcccaaaata ttaacttcag atggcgctta ctcggctgcg tgattctgcc taatcatgcc
     6241 tgaactttcg acttgccatg cactttcgtc catagattgt aaccgccgtt gaggttgact
     6301 gcccgaaaac catggttgcg gagtatgcga ttggcgaggt agccgcgcag acctatctgg
     6361 cagtaaatga cgtaggtctt gtccttgtcg agtgtaggga gtgcttcgcg gagttcatct
     6421 acattgaggt tgatggcacc tgtaatgtga ccttcggcgt attcgcgtgg tgtgcggaca
     6481 tccagaatga tggcattgtt ttcattaacg aatcggtcga attcgtcgta gttgaccata
     6541 tcgaggatgc cgttcaggat gttctctgca gtgtaaccaa cgatgttgac cggatctttg
     6601 gcagaattgt aaggcggagc gtaggtaatc tcgatctcgg gaaggtcgaa aatggtgaga
     6661 ttgcccttga tagctgtagc gatgacgtcg atacgcttgt caataccttt ctcgcctact
     6721 gcctgagcgc cgaatatgcg acccgaatcg tcaaagatga gtttgaggac aatgtttttg
     6781 gcgcccggat aataaccggc atgatgcccg cgcgtaacga tggctgtttt gtgcggaata
     6841 ttgtttcgct tcaggaactt ctcgctgagg ccggtacatg caacggtgag ttcgaagaat
     6901 ttgagtatgg aagaaccgag actgccagga taggtgtatc ggttgccaag tgaaatatta
     6961 tccgcgacca ttctgccttg tcggttggcc ggccatgcca gcggaatgag tactttgctg
     7021 tggtggatgt agtgctctac ctcgaccgca tcgcccacgg catagatgtc ggggtcagac
     7081 gtctgcatat agccgttgac gagtatgcca cctgtagtac ccgtttcgag tccggcatct
     7141 ttggcgagct tggtttcggg ttttacgccg atggccagga tgacagcttc ggtgtcaata
     7201 gatgtgccgt tgttaaggaa tacacggatg gtgtcgccaa ggtcggcaaa tttttcgaca
     7261 cctgtgttaa gtaataatgc caggccgtgt tgttcggctt tttcgtgaat gaagctggcg
     7321 atgtcgaagt caaccggagc catgagttgg ttgccaagtt ctacgagcgt tacctgcttg
     7381 cctgcttcaa ggagattttc agcaacttca agaccaatga agccgccgcc gaccactacg
     7441 aaagatttgg cagaccgggt tttcgagacg attttgtcca tgtcaggaat gttgcgcagc
     7501 gtgtaaatct tatcggagtc gataccttcg aatggcgggc gaatgggttc ggcaccggga
     7561 gacaagagaa gtttgtcgta tgattcggtg taagtgtcgc cggtgttcag atttttgatg
     7621 gagacagtct tagcattgcg atcaatagac aagacttcgg tatggatgcg aacgtcaaga
     7681 ttgtatcgtt ctttaaggct ttcgggtgtc tgtagcagta gtgcgtcgcg ttcgtggatg
     7741 acaccaccaa tatggtatgg aaggccacag ttggcaaaac tgatgtagct ccctttttca
     7801 aagatgatga cctcattttc ttcggataat cttcttaatc ttgtggctgc agtcgcacct
     7861 cccgcaacgc cgcctataat cagtattttc attgaaattt atattttgat acaaagatgg
     7921 tgtagatttg atatgctaat cggtaacaaa agtgacatgt acatcgaaaa tacgcgtttt
     7981 gatatggtaa aattaaagga tagtaataat gaaaggttgc tccctgacga aaatcatacg
     8041 tttgactgac gtaattcaga acaaaataaa gtatttatat acttttatta taaattttat
     8101 ccgtaacttt gcagaaggaa acgacaaaca aaaaacacat caacttaaga aaatgaaaaa
     8161 aacggctgaa aacatactag atgtaacggt cctagagcca tcgaagaagc atccgacgat
     8221 atttgcttgg tttgatgagc tggccggcgg tgaatgcttc atcattcata acgaccacga
     8281 ccccaagccg ttatattacc agatgtatgg cgagcgaggc ggtgtattca actgggaata
     8341 ccttgagcag ggtcccaaat ggtggaaagt gcgcatcaca aaacgaaggc acgacgaaac
     8401 tcagcagaca atagggcaga tggtagcaca agatgccaga aaagcagaaa tattccggaa
     8461 gtacaatata gcttatggtt cggatggaaa aaagatactg aatgaggcag caaaagagcg
     8521 agggcttgac gtgaccaaga tagaacaaga actacagcag cttgacagat caaaatacgc
     8581 tgaagctgca aagtacaacg aacttgatct tgatttcctg attgactaca tcatccagac
     8641 acaccatggc catgtaagaa gacagatacc caccataagg gaatatgcca gtaaagtgat
     8701 ggtcaaacat gctgccgggc atcctgagct gaataaaatc tacaagctgg tagaagaatt
     8761 gtacaccgag atatctacta acattcagaa ggaagaattg atcttgttcc cttacatcaa
     8821 agagatggta gcagcagcaa ggaacaacat cccatttgcc caaagtccgt ttgggtcggt
     8881 acagcagccg gtaaacatga tggagatgga acacgaagca gcgtgcagcc ttctgaaaga
     8941 aatacggcgg ctgagcctca act
//
LOCUS       DAOQQY010000090         8959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2966_length_8959_cov_4.709232, whole genome shotgun sequence.
ACCESSION   DAOQQY010000090 DAOQQY010000000
VERSION     DAOQQY010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2966_length_8959_cov_4.709232"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(33..1463)
                     /locus_tag="PLE23_05895"
     CDS             complement(33..1463)
                     /locus_tag="PLE23_05895"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMX82832.1"
                     /translation="MRIVKFLISLCITVFLAFTLSQPFKTGGKTLPPIGNFFSPFTGF
                     WQNSSSSFSLTGKKIKLPNCKQKVSIKMDNRMVPHIFAQNDHDLAYAQGYVTAMDRLW
                     QMDIATRAISGRLSEIIGTRTLETDKINRRKGHVYAAENAIAEWKKDPKLYSILESYA
                     QGINDYIAGLQEKDYPVEFKLLNYKPEAWTPLKTALFVISMAQTLAAHENDIENTNAL
                     KLFGQADYKDLYPERDPKEMPIIPAGTSWDYIKDTPALSKTQVDSTSIGDLFGIPRGI
                     TDDPHVGSNNWVVAGSKTKEGFPILCNDPHLHLTLPSIWYEVQLNAPGINSYGASLPG
                     VPGIIIGFNDDIAWGVTNSGWDVLDWYQIKWADNNKESYILDGKPLKVNYRIEKIIVK
                     DSLSYVDSVKYTIWGPVVYDDLKNPKHGLAMRWLVHTVHTPDEMATFFDLNRAHNFDE
                     YYSATQKYCAPAQNIVFASHEGDIAL"
     gene            complement(1693..2862)
                     /locus_tag="PLE23_05900"
     CDS             complement(1693..2862)
                     /locus_tag="PLE23_05900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMX82833.1"
                     /translation="MEAYVVKAFRSAVGKAGKGTLRNMRSDDLAVLVIKHLMEQTPEL
                     DPQLVDDVIVGCANPEGEQGLQIGRQISVRALGKSVPGMTVNRYCASGLETISIAAAK
                     INAGMGECFIAGGTESMSMIPMEGYKLAPSYDVAVNTPDYVVGMGLTAEAVAKKYNVS
                     REDQDIFSLRSHTLAAKAIERGFFKEEIVPVNVTEVWVQDNKRKEKSGIFDTDEGVRL
                     DTSLEGLSKLKPVFAMGGSVTAGNSSQTSDGAAFVLVMSERLVKQLNLQPIARLAACS
                     VAGVEPILMGIGPCAAIPKALHQAGLQLKDIDLIELNEAFAAQSLAVIREAGLDPDLT
                     NVNGGAIALGHPLGCTGAKLSTQLFGELKRRNKKYGLVTACVGGGQGIAGIWEGL"
     gene            complement(2869..5262)
                     /locus_tag="PLE23_05905"
     CDS             complement(2869..5262)
                     /locus_tag="PLE23_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMX82834.1"
                     /translation="MGTRHINKVAVIGSGIMGSGIACHLSNAGIEVVLLDIVTPGLSD
                     DQLRDKQARNQHVNQSLQNAIKSKPAPLMHSSFASRIKTGNIDDDLSMLKSCDWVIEV
                     VVERLDIKQQLYEKIEPYLKPGAIVSSNTSGIPIHFLSVGRSEAFRKNFVGTHFFNPP
                     RYLRLLEIIPTPDTDPELVSFLMAFGSKHLGKQTVLCKDTPAFIANRIGVYAMAKIFQ
                     LTEELNISLTDADKLTGPALGRPKTGTFRLGDLVGHDTAAKVIEGIRQNCPDDEQAGA
                     FTIPAYFRFLLDNKYFGNKSGQGFYKKTDQKDDKGKPIILSLNLKTHEYELPVSQKYQ
                     SLEVSKQIDSLGQRLKAIIKLEDAGAELIKRSLSGLFAYASNRVPEISDTLYSIDDAL
                     RAGFAWEVGPFEYWDLIGLDAGLSLIEKYGEKPAGWVLKMRESGNDSFYKASGGKVSC
                     YDVNTGTYAELPGASNIIKLEYFKDKKPVLENPEMVLHDIGDGILCLEFRSKSNAIGE
                     GILRGLDQAITLAEEDGWKGLVIGNNAQNFTVGANLMMIAMMAYQQEFDELNMAVNMF
                     QQTMMRCRYSSIPVVISTQGYVFGGGCEMLMHCDASAVAAESYIGLVEVGVGLIPGGG
                     GTKEFAVRTSDAFHKGDVMMPTLIEKFKTIATASVATSGYEGYDLGYLNHKDKVILNT
                     AGNIAEAKKIALQLSDGYQKPIIREDIYVLGRGGIATLYIAAHTLKLGNYASEHDIKI
                     AKKLAHVMCGGDLTGPQKVSEQYLLDLEREAFLSLCGEPKTLERIQSILTTNKPLRN"
     gene            5476..6180
                     /locus_tag="PLE23_05910"
     CDS             5476..6180
                     /locus_tag="PLE23_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMX82835.1"
                     /translation="MLAFIPLISSQADSSSVDQAVQGIGKQSIFDLIFSGGFSSIFIL
                     GLLLFLLFLAVAIFIERWLTIKRASKIDEYFMNNIRTNVQSGNLQAARQLCQQVDSPV
                     ARMVDKGLSRIGKPLRDIDAAIENVGNVELLKLEKNLPFLASISGISPMIGLLGTVSG
                     MILAFYEMATAQNVTPDVLAGGIYQALFATAGGLLNGIFALLGYNLLVSEIDKVVYKM
                     QLYSIEFMDLLQEPSK"
     gene            6189..6590
                     /locus_tag="PLE23_05915"
     CDS             6189..6590
                     /locus_tag="PLE23_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMX82836.1"
                     /translation="MGLNKRNRANPEFSLASLTDVIFLLLIFFMLTSTLVTQNALNLK
                     LPGANNRLVASPKLAVSVKNTGEYFLGSRASNLQEIESYIKNHVVGNSDPKQTTVTIL
                     ADKQCPIEYVVNVMDIANRLKVGAILATEPK"
     gene            6624..7514
                     /locus_tag="PLE23_05920"
     CDS             6624..7514
                     /locus_tag="PLE23_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82837.1"
                     /translation="MLAKFESTGERNNWNKALTISAAIHAILLILFFLPLLSFPDPPP
                     PKEGILVSLGRPDEGMGNDRPMAGNPNGSFEEKEIVNEPIEPTPPKPAPVPKSTPQPS
                     TPTPKVMTSDNAEDVSLKKAREDAKRREKAEIDARDKALREQREAEEAESKRIADENA
                     KRSAAKSKYGDMFKNGGTGRGNTGKPGNQGDPNGDPNSDILTGKSTGTGKVGGNIQAR
                     GGRGPTLTARVNDEGTVVIKVCIDARGKVISADYTQAGSTTNSSTLINLARDNASNYS
                     FKPSDQDKQCGTITYNFILK"
     gene            7540..8853
                     /locus_tag="PLE23_05925"
     CDS             7540..8853
                     /locus_tag="PLE23_05925"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HMX82838.1"
                     /translation="MTLSEQYKATIDYMYTRLPMFQRIGSAAYKKDLTNIRALCDFLG
                     QPQQQYKTIHVAGTNGKGSVSHMLSAIFQQKGLKTGLYVSPHYKDYRERIKVNGRYVS
                     KRFVIEFIDKLRPIIEQVNPSFFEINVAMAFEYFRQQKVDMAIIETGLGGRLDSTNII
                     DPVISVITNISFDHMSMLGNTLPEIAVEKAGIIKPDRPVVIGERQTDVQQVFTQKADE
                     LSCPILFASDHFTATTASSDLRHTFFDIYKDDRILAQHAPFNLHGTYQEKNIATVLGA
                     VQMLNSVADVHHLPVISLRDFLHAGKDLKALTRFMGRWQLLSAHPLTICDSGHNEAGI
                     RFIMQQLATIHYHQPHFVIGVVNDKDIQTMLALLPKDAVYYFCKANVPRGLNPNELRQ
                     IASTFGLLGKSYSSVRNALKAARKHAGPDDLVFVGGSTFVVAEVI"
BASE COUNT         2348 a         2208 c         1821 g         2582 t
ORIGIN      
        1 gatcttaacc gtgcacacaa ctttgatgaa tactatagtg cgatatcacc ttcgtgcgaa
       61 gcaaagacga tattttgggc aggcgcacaa tatttttgtg tcgcactata gtattcatca
      121 aagttgtgtg cacggttaag atcaaagaaa gtggccattt catcaggtgt gtgtacagta
      181 tgtaccagcc agcgcatggc aagtccgtgt ttcggattct tcaggtcatc atatacgaca
      241 gggccccaga tggtgtattt tacagagtca acgtagctaa gtgagtcttt tacaattatt
      301 ttttctatcc tgtagttgac tttgagtggt ttgccatcca gtatgtaact ttctttgtta
      361 ttatcagccc attttatttg ataccagtcg agcacatccc agccgctatt ggtgacgccc
      421 caggctatgt catcattgaa tcctatgatg atgccgggca ccccgggcag gcttgcacca
      481 taagagttga taccgggtgc attcagctgt acttcatacc agatggaagg tagagtcaag
      541 tgcaggtgtg ggtcattgca caagatcgga aacccttctt tggtcttgga gccggccacc
      601 acccagttgt tgctgcctac atgcggatca tcagtaatgc cccgtggtat cccaaataag
      661 tcaccgatac ttgtactgtc aacctgtgtt ttggataggg caggtgtatc ttttatatag
      721 tcccagctag tacctgcagg tatgattggc atttctttag ggtcgcgttc gggatacaga
      781 tctttgtaat ctgcttgccc gaaaagttta agagcattgg tattctctat atcattttca
      841 tgtgcagcaa gcgtctgtgc catagagata acaaagagtg cggttttcaa aggtgtccaa
      901 gcttcaggtt tatagttcag caatttgaat tctaccggat aatctttttc ctgaagacct
      961 gcaatataat cgttaatgcc ttgtgcgtat gattctaata ttgaatacaa ttttggatcc
     1021 tttttccatt ctgcaatagc gttttctgct gcatatacat gacctttacg gcggtttatt
     1081 ttgtctgttt cgagtgtacg cgtacctatt atctccgata atcttcccga aatggctctg
     1141 gttgctatat ccatttgcca aagtcggtcc atggcagtca catacccctg tgcataagcc
     1201 agatcatgat cgttttgagc gaaaatgtgc ggcaccatcc tgttatccat cttgatactt
     1261 actttttgct tacagttggg cagctttatc ttttttcctg tgaggctaaa gctcgaagac
     1321 gaattctgcc agaatccggt aaatggacta aaaaaattgc cgatgggtgg gagtgttttc
     1381 ccgcctgttt tgaatggctg tgaaagggta aatgcaagga atacagtaat acagagactt
     1441 atcagaaatt tgacaatacg cataaacaaa aatttttcgc attaaagtta ctttattttt
     1501 taatgccaca aagaatcctg atattattta tggaaaatat tttttgataa tatttattta
     1561 taaagactca ataaattcat tttttccttg attcaataaa tgtatgctcc taactttgac
     1621 accgataaat gatatttttt gtgtttattt gtttgggtta agtgcccttg ttgtttacaa
     1681 gggctatttt ttttacaatc cctcccatat tcctgctata ccttgtccgc ctccaacaca
     1741 agccgttacc agaccatatt tcttatttct tcttttgagt tcgccgaata gttgggtaga
     1801 aagtttggct cctgtacaac caagcggatg gccaagtgca attgctccac catttacatt
     1861 cgtaaggtcc ggatcaaggc ctgcctccct tatgactgca agtgattgtg ccgcaaatgc
     1921 ttcattcagt tctatcagat cgatatcttt taactgcaac cccgcctgat gaagtgcttt
     1981 tggtatggct gcacatggcc cgatacccat gagtataggc tccacacccg ctaccgaaca
     2041 agcggcaagt cgtgcaatgg gctgtagatt gagttgcttt accagccttt cgctcatgac
     2101 cagtacaaat gcagcaccat cactggtctg cgaactatta ccggctgtga ccgagccacc
     2161 cattgcaaat acgggtttga gcttggacag accttccaga ctggtgtcaa gccttacacc
     2221 ttcatcggta tcgaatattc ctgacttttc cttgcgttta ttgtcctgca cccatacttc
     2281 tgtaacattc acgggtacta tttcttcctt aaaaaaaccc cgctctatgg cttttgctgc
     2341 cagtgtgtgt gatcgtagtg aaaaaatatc ctgatcttcc cttgatacat tgtatttttt
     2401 tgcaacagct tctgctgtca aacccatacc tactacataa tcgggtgtat tgacagccac
     2461 gtcataactt ggagcgagtt tgtaaccttc cattggtatc atactcatgc tttcagtgcc
     2521 accggctata aagcattcgc ccattccggc gtttatcttg gctgccgcaa tagaaatggt
     2581 ttcaagccct gaagcacaat agcggttgac agtcataccc ggtacacttt tgcccaatgc
     2641 ccttacagat atttggcgac ctatctgaag gccttgttcg ccttcaggat tggcacagcc
     2701 tacgattaca tcgtccacca attgcggatc cagttctggt gtctgctcca tgagatgttt
     2761 tatgactaat acggccaaat catctgaacg catatttctc agtgtacctt ttccggcttt
     2821 tccaacagca cttctgaatg cttttactac ataagcttcc atattatatt aatttctgag
     2881 tggtttattg gttgttaaaa tggattgaat tctctctaag gtcttcggct caccgcacag
     2941 actcaggaat gcttctcttt ccagatcgag caggtattgt tccgacacct tctgtgggcc
     3001 ggtcaaatca cccccacaca tcacatgtgc cagttttttg gctattttta tatcatgttc
     3061 acttgcatag ttgcctaact tcaaagtatg ggctgctatg tacaatgtcg caattccacc
     3121 tcgaccaagt acataaatat cctctctgat gatgggtttt tggtatccgt ctgatagctg
     3181 caaggctatt ttcttcgcct ctgcaatatt gccggctgta ttcagtatta ctttgtcttt
     3241 gtggttcagg tatccaaggt catatccctc atagcctgat gtggcaactg aggcagttgc
     3301 tatagttttg aacttctcta tcagcgtagg catcatcaca tcacccttgt ggaatgcgtc
     3361 ggatgtccgt accgcaaact ctttggtgcc tccacctccg ggtataagtc caactcccac
     3421 ctccaccagg ccaatgtaag attcagccgc caccgctgat gcatcgcagt gcatgagcat
     3481 ttcgcaacca ccaccgaata catagccctg ggttgatatt accacaggta tagatgaata
     3541 acggcaacgc atcatggtct gctgaaacat attcaccgcc atgttaagct cgtcaaattc
     3601 ctgttggtaa gccatcatgg caatcatcat caagttggct cctactgtaa agttctgtgc
     3661 attgttacct attacaagac ccttccagcc gtcctcttct gctagtgtta ttgcctggtc
     3721 aagtccgcgt aatattcctt caccgattgc attgctctta ctcctgaact ccagacaaag
     3781 tattccgtct cctatatcat ggagcaccat ttctgggttt tcgagtaccg gctttttatc
     3841 tttgaaatat tcaagcttta tgatatttga tgctcccggc aactcagcat aagtgcctgt
     3901 attcacatca taacaactta ccttgccacc acttgctttg tagaatgaat cattccccga
     3961 ttccctcatt tttaacaccc agccagccgg cttctcaccg tatttttcaa tcaatgacaa
     4021 tcctgcatcc agaccgatca aatcccaata ttcgaagggg ccaacttccc atgcaaaacc
     4081 ggcacgcaat gcatcatcga tgctgtacaa agtatcggat atctccggta cacggttcga
     4141 ggcatatgca aataatcctg aaagtgatct tttgataagt tcggcaccgg cgtcctcgag
     4201 tttgatgatt gctttcagtc gctgaccaag cgaatctatc tgctttgata cttcgagcga
     4261 ctgatatttt tgtgataccg gaagctcata ttcatgtgtt ttaaggttca gtgatagtat
     4321 gattggtttg cccttatcat ctttttggtc ggtttttttg taaaatccct gacccgattt
     4381 gttaccgaaa tatttattgt caagcaaaaa acggaaataa gccggtatgg taaaggcccc
     4441 ggcttgctca tcatccgggc agttttgcct gataccttct attaccttgg ccgccgtatc
     4501 atgcccaaca agatcgccca gcctgaatgt gcctgtctta ggtctgccaa gcgcaggacc
     4561 tgtaagttta tctgcatccg ttaatgaaat gttgagttct tctgtcaact ggaatatttt
     4621 tgccatagca tacacaccga tgcggttggc aataaatgcc ggtgtatcct tgcacaaaac
     4681 cgtttgcttg cccaggtgtt tcgatccaaa tgccatcagg aaactgacaa gctccgggtc
     4741 ggtatcaggt gtcggaataa tctctaacag cctcaggtat cttggcggat tgaaaaagtg
     4801 ggtgcccaca aaattctttc ggaacgcctc acttctacct actgaaagaa agtgaatagg
     4861 aataccggac gtattggatg acacgatggc tccaggcttc aggtaaggtt ctatcttttc
     4921 gtacagctgt tgcttaatgt caagcctttc tacgactact tctattaccc agtcgcaact
     4981 ctttagcata ctcagatcat cgtctatatt gcccgttttt atccttgagg caaaactact
     5041 gtgcatcaaa ggtgccggtt tcgacttgat ggcattctgt agcgattggt tgacatgctg
     5101 gtttctggct tgcttgtctc tgagctggtc atcactcagc cccggtgtga cgatatcgag
     5161 cagaactact tcaatacccg cattggacag atgacaggct atacctgaac ccataatgcc
     5221 cgacccgatg accgctacct tattaatatg tcttgttccc atatattgtc atttagcgaa
     5281 ttttcgcaaa tttaagaaat ttttatgatt ggatgcaatt ttaattatca aataatgatt
     5341 gcacttaaca tatatttatt tcagaatcaa taattgagtg ctcaataatt ttaaaattga
     5401 aaaattaaat taattttacg cccgtattta taatttcggc ttgaccgaat tcgtttcaaa
     5461 acaaatttta aaaccatgtt agcattcatt ccgttgatat cttcacaggc agattcttca
     5521 tccgttgacc aggcagtaca aggtatcggc aagcaaagta tctttgatct cattttttcc
     5581 ggtgggtttt cttccatctt tatcctcggc ctcttgttgt ttctactctt tctcgcagta
     5641 gccatcttta tcgaacgatg gcttacgatc aagcgtgcat caaaaataga tgaatatttc
     5701 atgaacaaca tccgcaccaa tgtacaaagt ggcaatctgc aggcagcaag gcaactttgt
     5761 cagcaagtgg acagcccggt ggcacgtatg gtggataaag gtctttcgag aatcggcaaa
     5821 ccgctcaggg atattgacgc agccatagag aatgtcggta atgttgaatt gttaaaactc
     5881 gaaaaaaacc tgccgttcct cgctagtatc tcaggtattt cgcctatgat tggtctgctt
     5941 ggtactgtat cgggtatgat tctggcattt tatgagatgg caactgctca aaacgtcacg
     6001 cctgatgtgc ttgccggtgg tatttaccag gctctctttg ctactgcagg tggtctgctc
     6061 aatggtatat tcgccctcct gggctacaac ctacttgtat ccgaaatcga caaggtagtt
     6121 tataaaatgc agctttattc tattgagttc atggatttgc tacaggaacc ttctaaataa
     6181 tattcgccat gggcctaaac aaaagaaaca gagcaaaccc ggaattcagt ctggcatccc
     6241 tcacagatgt tatcttcctg ctattgatat tcttcatgct tacctctacg cttgtaacgc
     6301 agaatgcact caacctcaaa cttcccggcg ccaacaaccg cctggtagct tcgcctaagt
     6361 tggctgtgtc cgttaaaaat accggtgaat attttctggg tagccgtgcg tccaatctgc
     6421 aggaaattga atcatatatt aaaaaccacg tagtaggcaa cagcgatcct aaacaaacta
     6481 ccgttaccat actggctgac aaacaatgcc cgatcgaata cgtagtaaat gttatggata
     6541 ttgccaaccg cctcaaagtg ggtgcaatcc tggcaacaga accaaagtaa tgattatttt
     6601 gttttagatt taataacatc ggcatgttag caaaattcga atcaacaggt gaacgcaata
     6661 actggaacaa ggcactgacc atttcggcag ctattcatgc cattctgctg attttgttct
     6721 tcttgccgct gttgagtttt cccgacccgc ctccacctaa ggaaggtatt ctggtatcac
     6781 tcggcagacc cgatgaaggt atgggtaatg atcgtcctat ggcaggcaat cccaatggct
     6841 ctttcgaaga aaaagaaatt gtcaacgaac ccattgaacc aactccaccg aagcctgctc
     6901 cggttccaaa atctacacct caaccatcaa ctccgacgcc taaagtaatg acatctgaca
     6961 atgccgaaga cgtctctctc aaaaaagcac gcgaagatgc caaacgtaga gaaaaagccg
     7021 aaatagatgc tcgtgataaa gccctgcgcg aacaacgtga ggccgaagaa gctgaaagta
     7081 aacgcatcgc tgacgaaaat gcaaaaagaa gcgccgctaa atcaaaatac ggcgatatgt
     7141 tcaaaaacgg gggcaccggc cgtggcaaca ccggcaaacc gggcaatcag ggcgacccta
     7201 acggcgaccc caactccgat attctcacag gcaaatcaac cggcacagga aaagtaggtg
     7261 gcaatattca ggcaagaggt ggccgtggtc ctacgcttac tgctcgtgtc aacgacgaag
     7321 gtactgtagt catcaaggtt tgtattgatg cacgtggcaa agtcatttct gccgattaca
     7381 ctcaggccgg ctctactacc aacagctcga ccctcattaa tctggccaga gataatgcaa
     7441 gcaattatag cttcaagcct agtgatcagg ataaacagtg cggtaccatt acctacaact
     7501 tcattcttaa ataatttcga cttctaacac atacccggtt tgacgctgtc cgaacaatac
     7561 aaggccacca tagattatat gtacacacgt ctgcctatgt ttcaacgcat tggcagtgcc
     7621 gcttacaaga aggatcttac taacatccgc gccttgtgcg atttcctcgg acagccacag
     7681 cagcaataca aaaccatcca cgtagctggt accaacggca agggctcggt gtcacacatg
     7741 ctcagtgcca tctttcagca aaaaggcctc aaaaccggcc tctatgtatc gccacactac
     7801 aaagactacc gcgaacgcat caaggtcaac ggccgctacg tgagtaaacg attcgtcatc
     7861 gagtttatcg acaagctgcg ccccatcatc gagcaggtca atccatcctt ctttgaaatc
     7921 aatgtggcta tggcttttga atacttccgc caacaaaaag tggacatggc tatcatcgaa
     7981 accggactcg gcggcagact cgattccacc aacatcatcg atcctgtcat cagcgtcatc
     8041 accaatatca gcttcgacca catgagcatg ctcggcaaca cactgccgga gatagcagta
     8101 gaaaaagccg gtatcatcaa gccagacagg cccgtggtta taggcgaacg tcagactgat
     8161 gtgcagcagg tattcaccca aaaagcagat gagctcagct gcccaatctt gtttgcgtcc
     8221 gatcatttta ccgccacaac tgcctcatcc gatctccggc acacattttt cgatatatac
     8281 aaggacgacc gcatcttagc acagcacgca ccgttcaatc tacacggcac ataccaggaa
     8341 aaaaacatcg ccacagtact cggcgctgta caaatgctaa attccgtcgc cgacgttcat
     8401 cacttgccgg tcattagctt acgtgatttc ctccatgccg gcaaagatct caaggcactt
     8461 accaggttta tgggcaggtg gcaactgctc tccgctcacc cgctcaccat ctgcgatagc
     8521 ggccacaacg aagcaggcat acgcttcatc atgcaacagc tggccaccat tcactaccac
     8581 caaccgcatt tcgtcatagg cgtggtcaac gataaagata ttcagacaat gctggcattg
     8641 ttgcccaaag atgcagtgta ttatttctgc aaagcaaatg tgccaagagg ccttaaccca
     8701 aatgaattaa gacaaatcgc ctccaccttc gggctgctcg gcaaaagcta ctcctccgtc
     8761 cgcaatgcac ttaaggctgc aagaaaacac gccggccccg acgaccttgt tttcgttggc
     8821 ggtagtacct ttgtagtagc cgaagtcata taaccaaaca ccgacacaat cattctacat
     8881 atctctgtgc atctgttttc atttgggtgt gcccctgtcg ggtcgggcta tccgccttac
     8941 ttcgtgccgg cttctatcc
//
LOCUS       DAOQQY010000091         8948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2974_length_8948_cov_5.158889, whole genome shotgun sequence.
ACCESSION   DAOQQY010000091 DAOQQY010000000
VERSION     DAOQQY010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8948
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2974_length_8948_cov_5.158889"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3909
                     /locus_tag="PLE23_05930"
     CDS             <1..3909
                     /locus_tag="PLE23_05930"
                     /inference="COORDINATES: protein motif:HMM:NF028519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HMX82839.1"
                     /translation="SYSWSNGATQKDISALVSGAYTVTVTDTKGCSAVKSFIVNQATE
                     LIVNGTSKNPTCFNGADGAINLTVAGGTPGYAYTWSNGAFTQNLSGISAGNYSVTVTD
                     ANGCKKTASFTLVNPSEIQITGIITNAACSKSTGSINITVSGGTPGYNYKWSNGSTSE
                     DLSNLLAGNYTVTVTDSKGCNKIATFTVNDKGGINLFGTVVDVSCFGGNDGSASISIS
                     NATLPVAINWSNGASNVTNISNLVAGNYSVTVTDAIACVEVKNFIIQQPPVISVSGNV
                     SNVMCNGQNTGSVSLTVSGGTPGYTFQWSNGASTQNISGLAAGNYSVTVTDSKGCKSI
                     HSYTVAQPLVLSVSGIIVNPTCYNGANGSISITVSGGTPNFAYNWSNGSTTKDISGLS
                     AGNYSITVTDANGCSKTTSFTISNPTEIQVTGQINHATCSQANGNITITVSGGAPGYT
                     YAWSNGANTKDISNLAAGTYNVTVTDVNGCTKIKTFTVDNLAGPSINGTVVNVSCDGG
                     NNGSVSITVSGGTNPYSTVWSNGSVNTFTISNLTAGTYSVTVTDSKQCTAVQSFTVAQ
                     PAVLGVSITKTNISCNNGSNGSLTANVSGGTPVYSYKWSNGQTTQQIQNLIPGTYTVT
                     VTDAHNCTSTASATLTNPPALVVSFMAGKTSCYNSADASIILTVSGGTSPYSYLWENG
                     STSKDRFNLAKGNYSVTITDFAGCTKIENIPIFSPIQMTFDLQVTHQTCLDKNDGKIS
                     VTNLLGGVPNYTYKWNNPGGSTTPTISNLAPGTYTVTITDVKGCTEVKSATVNPSNLK
                     CTVNIGDYVWLDEDKDGIQDANEMGVNGIVVHLINLGPDGILGTGDDIIVDTEYTKTK
                     GGTKGYYYFPDVPAGTYVIMFFADKDKYNYSPQYQGSDPAKDSNVYPSTGKTDIFTVN
                     VGDQDDLRFDAGIYVYCDNVTSGGVICCNETICKIGGTPSLIESVLPASGGSGGPIEY
                     VWNKSTKNPYYYPNSPDWEMIPNSNTPSYQPGPITETTYYIRCSRRAGCGPFVGEANI
                     VTKKVVDAKQADILDAPPIICYNMPITLNSIDYGAGVTYEWHLGYGAVPATATTPSVL
                     VNWPTPGLKNIQLKVTTDGCVSTDIAIVSVESCKDMVVNNFTAILNSELSVDLKWRLD
                     NTGSNLFEILRSQDNVNFVAINSLKGFPDKDGYYRILDKKPEIGMNYYQIKAYAANGL
                     YTISETRSVLVNDTDNRNVVVYPNPASDYITIEPLESDLGATEIQLTDVAGRVLKSYK
                     LSAGFKQFDVDVSSLASGVYFINVKTEGKRPFTYKVIKQE"
     gene            complement(4022..4525)
                     /locus_tag="PLE23_05935"
     CDS             complement(4022..4525)
                     /locus_tag="PLE23_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82840.1"
                     /translation="MNVSIPVSFPDLSAFDDQSRIWLFQLDRPLSDEVCNLIYNDVKT
                     YVDSWQSHGDKVQATTAVIANRFILLLADERYTSVGGCSTDVMMRFIQHLQAEYKVNL
                     LDRFLVYYIDGEEILSANMHVLLQKIRNEELPADILIFDALITTKSEFDSNWVKPLNQ
                     SWVKRFL"
     gene            complement(4533..5309)
                     /locus_tag="PLE23_05940"
     CDS             complement(4533..5309)
                     /locus_tag="PLE23_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMX82841.1"
                     /translation="MKIYTMSEMASEGKSPEVLFWVGCAGSFDTRAQRVTKAFCEILN
                     RAGISFAVLGNEERCTGDPARRAGNEFLFQMMAQQNIQTLNMYNVKRIVTACPHCFNI
                     LKNEYPELGGVYDVIHHTQFLETLLKEGKIKVDEQSEVTTVTYHDSCYIGRGNNIYDT
                     PREVLEMLRVELKEMKRSRANGLCCGAGGAQMFKEEEAGDKRINTERAEEIIGTGTNI
                     VAANCPFCITMLQDGIKEKNEQDNIIVLDLAELVLQKMNK"
     gene            complement(5326..6642)
                     /locus_tag="PLE23_05945"
     CDS             complement(5326..6642)
                     /locus_tag="PLE23_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HMX82842.1"
                     /translation="MSQLLFLIILLAVAYFATKKFIRIKDNIMLGLPYNISGDSAERW
                     KRMILIAFGQKKMFARWIPAVMHLFIYVAFLFTQIELIEIIIDGLAGTHRFFADKIGV
                     LYNVLINFIEFLSLGAFVATIVFLSRRNIIRLARFQKPEMKGWPSKDANIILLLEITL
                     IIGIFSMNGADVVLQSIDPEHFKSTGHLFVSSHLGPALFGGLDAGTLHIVERFGWWLH
                     ILVVFGFVLYLPYSKHLHIFLSFPNVYFSNLHPSSKMENMPEIMNEVKSMFGLETDGV
                     GADMSSGELPVFGSNDVTNLSWKNLMDAYSCTECGRCTSVCPANITGKKLSPRKIMMD
                     VRDRAEVVGNEIFSKGIKKEEFNDGKSLFDSINREEIHACTSCQACVEACPVLINPLE
                     IILKLRRYEILTESAGPAEWLPMFNAIENAGSPWQMSVERDEWIRS"
     gene            6787..7281
                     /locus_tag="PLE23_05950"
     CDS             6787..7281
                     /locus_tag="PLE23_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HMX82843.1"
                     /translation="MKLLGHIDILRKIKRITIEILEKNYQEKEIILAGINKNGTAFAK
                     LLFDECTKRGNQQYTLTTITLSPANPLERKVEAGLPVEYFTDKLIIIVDDVANTGRTI
                     FYAIKPFLDVLPGKVEVAVLVDRKHKSFPVKVDYMGLSLATTLQENISVNLSEKDNYS
                     VYLD"
     gene            complement(7346..>8948)
                     /locus_tag="PLE23_05955"
     CDS             complement(7346..>8948)
                     /locus_tag="PLE23_05955"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HMX82844.1"
                     /translation="YVVDDIADLSTFGIGSANNGNGSNGVEFAFPAVSATKGSFIYLA
                     ADSALFHQYFGFDADYISSAMNINGDDAIELFENATVIDVFGEIDVDGTGKPWEYTDG
                     WAYRNNGTGPDGSLFVIANWHFGGVGSLDGAPTNAEVANPFPIGTYSTKSDSLTICKD
                     DLVTTDFETSVLIDILKNDILPNGVKAVYITTPLNGQVILDNNNNVTYTPFDGFCGQD
                     GFIYILEDNNSTLDTAEVFITVNCASTYPAYDIATVTTVDANGLPDSLNVTCELGGII
                     YGNDFQGSAALQFYLIDNTGGISVFSSNAFGYTVKEGDEVRVRGVITHFSGLTQISAD
                     TLWLVSENNPLKAPTVVTTLSEATESEFVKINKLTFKDKSQWKTGQGSGFSVDLLDGA
                     GNTIVMRIDNDIDLFKEPAPSYTWLNVTGIGSQFDNTAPYNSGYQILPRYKSDLEGLV
                     STNDQGLDSKIIMYPNPTVGKIGIRTDLKLNGIVVTNQLGTEVLQISGDDFNKSYDLT
                     QLPAGIYFVTFKMNDTQVTKKLIRF"
BASE COUNT         2750 a         1868 c         1719 g         2611 t
ORIGIN      
        1 agttatagct ggagcaatgg tgcaacacaa aaagatattt cagccttggt ttcaggtgca
       61 tatactgtaa ccgttaccga cacaaaaggg tgttctgctg ttaagtcttt catcgtcaac
      121 caagctaccg aacttatagt aaacggaact tccaagaacc caacctgctt taacggtgct
      181 gatggtgcaa tcaatctgac agttgcagga ggaactccgg gctatgcata tacatggagt
      241 aatggtgcat ttacccagaa tttaagtggt atttctgccg gcaattattc cgtaaccgta
      301 acggatgcca atggttgtaa aaaaacagcg tcattcacat tagttaatcc ttcagaaata
      361 cagattaccg gtatcatcac taatgctgcc tgcagcaaga gtaccggatc aattaatatt
      421 acagtaagtg gcggtacacc tggatataat tacaagtggt ctaacggatc cactagtgaa
      481 gatttgtcca atttacttgc tggtaattat acagttactg taactgattc aaaaggctgt
      541 aataaaattg ccacattcac agtaaatgat aagggtggta ttaacttgtt cggaacagta
      601 gtagatgtaa gttgttttgg cggaaatgac ggttcagcat ctataagtat ttcgaatgca
      661 actttacctg tcgctattaa ctggagcaac ggtgcgagca atgtcactaa catttccaac
      721 cttgttgccg gcaactacag cgtaactgtg acggatgcta tagcttgtgt agaagttaag
      781 aatttcatca ttcaacagcc tcctgttatc tctgtttcag gtaatgtatc aaatgttatg
      841 tgcaatggac agaatacagg ttcggtttca ctaacagtat caggtggtac accgggttat
      901 accttccagt ggagtaatgg agcgagtaca cagaatattt cagggcttgc agcaggaaat
      961 tactcggtaa cagttactga cagcaaaggg tgcaagtcta tccattctta cacggttgca
     1021 caaccattgg tattgtcagt gtccggaata attgtaaatc ctacctgtta taatggtgct
     1081 aatggttcta tttcaattac agtttcgggt ggcacgccta attttgctta taattggagc
     1141 aatggctcaa ccactaaaga tatttcaggt ctaagtgccg gtaattatag cattactgta
     1201 acagatgcaa atggatgtag caaaactact tcatttacca ttagtaatcc gacagaaata
     1261 caggtaacag gtcagataaa ccatgctaca tgtagtcagg caaatggcaa tataaccatt
     1321 accgtgtcag gaggcgcacc cggatatact tatgcctggt caaatggtgc aaacaccaaa
     1381 gacatatcga atctggctgc aggaacgtat aatgttacag ttacggatgt caatggatgt
     1441 acaaaaatta agacattcac agttgataac ctggccggac cttcgataaa cgggacagtg
     1501 gttaatgtta gctgtgatgg aggcaacaac ggttcagttt caataacggt gtcaggtggc
     1561 acaaatccat attcgacagt atggagcaat ggttccgtaa atacattcac aatatctaac
     1621 cttaccgcag gaacctatag tgttacagtt actgacagca agcaatgtac tgctgtccaa
     1681 agctttacag tagctcagcc agctgtactt ggtgtttcta tcacgaaaac aaacatcagt
     1741 tgtaataatg gttcgaatgg ttcactaact gcaaatgtat ctggtggcac gcctgtatat
     1801 tcatacaaat ggagcaatgg tcagactacc caacaaatac aaaatctgat acctggcact
     1861 tatactgtga ccgttaccga tgcccacaat tgtacttcta ctgcaagtgc tacattgaca
     1921 aatccacctg cattagtagt aagtttcatg gcaggcaaaa cttcgtgcta caattcagca
     1981 gatgcttcta ttattctaac agttagtggt ggcacatcac catatagcta tctttgggaa
     2041 aatggatcaa catctaaaga cagatttaac ctggccaaag gaaattattc agtaacaatt
     2101 actgattttg caggctgtac gaagattgaa aatattccga ttttctcacc tatacagatg
     2161 acttttgacc ttcaggtgac acaccagacc tgtcttgata aaaacgacgg aaaaatttct
     2221 gtaactaatc ttctcggtgg tgttccgaac tatacctaca aatggaataa tcctggcggt
     2281 tcgaccacac cgactatttc gaatcttgca ccgggcactt ataccgtaac gattacagat
     2341 gttaaaggat gtaccgaagt aaaatccgca actgtgaacc catcaaacct aaaatgtact
     2401 gttaacatag gtgactatgt atggctcgat gaagataagg atggcattca ggatgcaaat
     2461 gaaatgggtg ttaacggtat cgttgttcac ttaattaacc ttggtcctga cggaatcctc
     2521 ggtacaggtg atgacatcat tgtggataca gaatatacta aaacaaaggg aggtaccaaa
     2581 ggatattatt atttcccaga tgttcctgca ggtacttatg tcatcatgtt ctttgctgac
     2641 aaggacaagt acaactattc accacaatat cagggttctg atccggcaaa agacagtaat
     2701 gtctatcctt caaccggcaa gacagacata ttcactgtta atgtcggtga ccaggatgat
     2761 ctgagatttg atgccggtat ctatgtatat tgtgacaatg taaccagtgg tggcgtcatt
     2821 tgttgtaacg agacaatttg caaaatcggt ggtacacctt cgttgataga aagcgtactt
     2881 ccggcttcag gtggatcagg tggtccaatc gaatatgtat ggaacaaatc aaccaaaaac
     2941 ccttactatt atcctaatag cccggactgg gagatgatcc ctaactcgaa tacaccttcg
     3001 taccaacctg gccctattac agaaacaact tactacatca gatgctcaag aagagcaggt
     3061 tgtggtccat ttgtaggtga agctaatatt gtgactaaaa aagttgtgga tgccaagcag
     3121 gcagatatcc tggatgctcc tcctatcatc tgttacaaca tgccaataac actcaactcc
     3181 attgattacg gtgcgggtgt gacttacgaa tggcacttgg gctatggtgc tgtacctgct
     3241 actgctacca caccatctgt acttgtcaat tggccaactc caggtttaaa aaacattcag
     3301 ttaaaagtaa ctacagacgg ttgtgtctct acagatattg ctattgtgtc agttgaaagc
     3361 tgtaaagata tggtggtaaa taactttact gctatcctta acagcgagct aagtgttgat
     3421 ttgaaatgga gacttgataa tacaggcagc aatttgtttg agatacttcg ttcgcaggat
     3481 aatgtcaatt tcgttgctat caattctcta aaaggcttcc ctgacaagga cggctactac
     3541 agaatattgg ataaaaagcc ggaaattggc atgaattatt accagataaa agcctatgct
     3601 gcaaacggac tctatacgat ttctgaaacc agaagtgtgc ttgtgaatga tacagacaat
     3661 agaaatgtag tcgtgtatcc aaaccctgct tcggattaca tcactataga gccacttgaa
     3721 agcgaccttg gcgctacaga aatacaactc acagatgtag ctggccgtgt acttaaatca
     3781 tacaaactaa gtgcaggatt caagcagttt gatgtggatg tgagtagctt ggcttcaggt
     3841 gtatatttca tcaatgtaaa aaccgaaggg aaacgtccgt ttacttataa agtaataaaa
     3901 caggaataat acattcaata ttaatctaaa taaaaaagag atgaccaaat ggttcatctc
     3961 ttttttttga aagtaaagtt acaaagttct tttcctgaga gttgttggat attctttcag
     4021 cctaaaggaa tctttttacc catgactggt taagtggttt aacccagtta gagtcaaatt
     4081 ccgatttggt ggttataagt gcgtcaaaaa tcaaaatatc ggccggtagt tcttcgttgc
     4141 gtattttttg tagaagcacg tgcatatttg ctgacaagat ttcttctcca tcaatgtagt
     4201 aaacaagaaa cctatcgagc aaattgacct tatactcagc ctgcaaatgc tgaataaaac
     4261 gcatcatcac atctgttgaa cagccaccta ctgaagtgta tctttcatct gcgagtagta
     4321 aaataaacct attggcaatg actgctgtgg tagcttgtac tttgtcgcca tgactctgcc
     4381 acgaatcgac ataagtttta acatcattat agattaaatt acacacctca tccgacaaag
     4441 gcctatcaag ctgaaatagc caaattcttg attgatcgtc aaatgcggac aaatcgggaa
     4501 aacttacagg aatgcttaca ttcatttcca tattatttat tcattttttg aagtaccagt
     4561 tcagccaggt ccaatactat gatattatct tgttcattct tttccttaat accatcctgc
     4621 aacatagtga tgcaaaaagg gcagttggct gcgacaatgt tggtacctgt acctatgatt
     4681 tcttcagctc tttctgtatt tatcctttta tcaccagctt cttcttcttt aaacatctgt
     4741 gctcctccgg caccacaaca aagcccgttt gcacggctac gcttcatttc tttcaattca
     4801 accctcagca tttcaagcac ttcacgaggc gtatcgtaga tgttatttcc tctgccgata
     4861 tagcatgaat catggtacgt aacagtcgtt acctcagatt gttcatcaac tttgatcttt
     4921 ccttctttaa gtagcgtctc aagaaactgc gtgtggtgga tgacatcata cactccgccg
     4981 agttccggat attcgttttt gagtatgtta aaacagtgag gacatgcagt gacaattctc
     5041 ttcacattat acatattaag tgtctggatg ttctgctgag ccatcatctg aaacaaaaac
     5101 tcgttgcccg cccttcttgc aggatcgcct gtacaacgtt cttcatttcc aagaacggca
     5161 aatgaaatac ctgcacggtt caatatttcg caaaatgcct tggtgacacg ctgtgctctt
     5221 gtatcaaaac ttccggcaca tcctacccaa aacaacactt caggtgattt tccttcactt
     5281 gccatttctg acatggtgta tatcttcata agtatttggt ttatattatg atctgatcca
     5341 ttcatctctc tcaacagaca tctgccacgg gctacctgca ttttctattg cattaaacat
     5401 tggaagccat tcagctggcc cggccgactc tgtaaggatt tcatatcttc ttaattttag
     5461 tattatctca agcgggttaa tcaacacagg acaagcttct acacaagcct gacaactagt
     5521 acacgcgtga atttcttctc tgttaattga atcgaagagt gatttcccgt cgttgaattc
     5581 ttcttttttg attcctttag aaaaaatttc attaccaacc acttctgccc tatctcttac
     5641 gtccatcatg atttttcgtg gagaaagttt ttttccggta atatttgccg ggcaaacaga
     5701 tgtacacctg ccgcattcgg tacacgaata agcatccatc aggttcttcc agcttaggtt
     5761 tgtcacgtca ttcgatccga atacaggcaa ctcgcctgat gacatatcag cacctacacc
     5821 atctgtttcg agcccgaaca ttgacttcac ttcattcatt atctcaggca tattttccat
     5881 cttggatgaa ggatgcagat tggaaaaata aacatttgga aaacttagaa atatatgaag
     5941 gtgcttcgaa taaggcagat acaaaacaaa accaaagact acaagaatat gaagccacca
     6001 gccgaatctt tctacgatgt gcagtgtacc ggcatcaagt ccaccaaaca atgctggtcc
     6061 gagatgcgag ctcacaaaca gatgccctgt tgatttaaaa tgttcaggat caatgctttg
     6121 caacacaaca tctgcgccat tcatcgaaaa gatgccaatg atgagcgtaa tctccagcaa
     6181 taagataata ttggcgtcct ttgaaggcca tcctttcatt tcaggttttt gaaatcttgc
     6241 aagcctaatg atatttctcc ttgacaggaa aacgatggtt gcaacaaaag ctccaagtga
     6301 aaggaattca ataaaattga tgagtacatt gtacaaaacg ccaattttat cagcgaaaaa
     6361 acgatgtgtc ccggcaaggc cgtcgataat gatttcgatg agttcaatct gtgtaaacag
     6421 gaatgctaca taaataaaca agtgcataac tgcgggtatc catctagcaa acattttctt
     6481 ttgcccaaaa gctatcagta tcatacgttt ccatcgctct gcactatctc cgcttatatt
     6541 gtaagggaga ccgagcatga tattgtcttt gatgcggata aatttcttag tggcgaaata
     6601 agctacagcc aaaagaatga tcaggaatag cagttgtgac atacctgtat ttttaaattc
     6661 gtgctaaagt taaaacccat ttatctaaaa tgccaacaca tttacagaaa tataaattat
     6721 ttttagtgaa aaaagtatta ctgctaagca tttttctcaa tatgatttaa ttttacacaa
     6781 aaaagcatga agctactcgg gcatattgac attctcagga aaataaaaag gataacaatc
     6841 gaaattcttg aaaaaaatta tcaggaaaag gaaatcatac ttgcaggcat aaacaaaaac
     6901 ggaactgctt ttgcaaaatt actatttgat gaatgtacaa aacgtggtaa tcagcaatat
     6961 acgcttacta ccataacact cagccctgct aaccctctcg aacgaaaagt agaggccggg
     7021 ctgcctgttg aatattttac tgataaactg atcattattg ttgatgacgt ggccaatacc
     7081 ggtcgtacta ttttttatgc catcaagcca ttcctggatg ttttgccagg caaagtagaa
     7141 gtagctgtcc tggttgacag aaagcacaaa tcttttccgg tgaaagttga ttacatgggt
     7201 ttatcgttag caaccaccct gcaggaaaac attagtgtaa atcttagcga aaaggataat
     7261 tattccgttt atctggattg attctccttc ataacagaaa aaaacagagc cacaatatac
     7321 tcatggctct gttttgaaag tgtctttaaa acctaatcaa tttttttgtc acttgcgtgt
     7381 cattcatttt aaatgtcacg aaatagatac ctgccggtaa ttgtgttaaa tcataggact
     7441 tgttaaaatc atctccactt atctgcaaaa cctcagtgcc caattggttt gtcactacta
     7501 taccattcag cttcaaatcg gttctgatgc caattttccc aacagtcggg tttggataca
     7561 tgatgatttt tgaatcgagc ccttgatcgt tggttgacac aagcccttca agatcagatt
     7621 tatatcttgg aagaatctga tagccagaat tatatggtgc agtattatca aattgacttc
     7681 ctatgcctgt tacattgagc caagtataag aaggtgccgg ctctttaaac aaatctatgt
     7741 cgttgtctat tcgcattacg atagtattgc ctgcaccgtc caacaaatca actgaaaaac
     7801 cagaaccttg tcctgtcttc cattgcgatt tgtctttgaa tgtcagttta ttgattttta
     7861 caaactcaga ctcagttgct tcagataagg tagtaacaac tgtaggcgct ttgagcggat
     7921 tattctcaga caccagccaa agagtatctg cacttatttg tgtcaacccg gagaaatgtg
     7981 ttataacacc tctgacgcgc acctcatccc cttcctttac agtatagcca aaggcattgc
     8041 tactaaacac tgaaatacca ccggtattat cgatgaggta aaactggagt gctgcactac
     8101 cctggaagtc attgccatag atgataccac ccagctcaca ggttacatta agtgaatcag
     8161 gcaaaccgtt agcatccacg gttgtaacag ttgcaatatc ataagccgga taggtagagg
     8221 cacagttaac agtaatgaat acctcagcag tatcgagtgt agagttattg tcttcaagaa
     8281 tgtaaatgaa accatcctgc ccacagaatc cgtcaaacgg tgtatatgta acgttgttgt
     8341 tattgtcaag tatcacttgg ccattcaacg gtgtagtaat atacacagcc ttcactccgt
     8401 taggcaatat gtcgttttta agaatatcaa tcaaaacaga tgtttcgaaa tctgtagtga
     8461 ccaaatcatc cttacatata gtaagtgaat cagatttagt tgaataagta ccaattggga
     8521 aaggattggc aacctctgca ttagtaggtg cgccgtcaag tgaacctaca ccgccgaaat
     8581 gccagttggc aattacgaac aagctaccgt caggtcctgt tccattattg cggtatgccc
     8641 atccatcggt gtattcccaa ggtttgccgg ttccatctac atctatttca ccaaacacat
     8701 caataacggt agcgttctca aataattcga ttgcatcatc tccattgata ttcattgctg
     8761 aagaaatata atcggcatca aatccaaaat attgatggaa caatgcgcta tccgccgcaa
     8821 ggtaaataaa tgatcctttg gtggccgaaa ccgccgggaa tgcaaactct acaccgttac
     8881 tgccattgcc gttatttgca gagccgatac caaatgtgct caaatcggca atatcatcta
     8941 caacatac
//
LOCUS       DAOQQY010000092         8937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2985_length_8937_cov_5.976244, whole genome shotgun sequence.
ACCESSION   DAOQQY010000092 DAOQQY010000000
VERSION     DAOQQY010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8937
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2985_length_8937_cov_5.976244"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2385
                     /locus_tag="PLE23_05960"
     CDS             <1..2385
                     /locus_tag="PLE23_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMX82845.1"
                     /translation="EHKTRALFVPQDQAWFDIGGSTTPTNCGDHGSHCLGTILGLDRL
                     NNDTIGVAFNAQWMGGKILCGGGTYGILQALQWAINPDGNKNTTVDMPDVINNSWYDP
                     GVANDCESGYVDVEAALEAAGIASIFSAGNAGPDEATITPPHNVNISVVNSFTIGAVN
                     GNVSSLPIADFSSRGPSKCGGTGSVLIKPEVSAPGVSVRSCTFDKAYDLKSGTSMAAP
                     HTCGAILLLKEAFPYLSGYDFKMALYNTCTDLGVPGEDNVYGQGIINVWAAYNYLIAQ
                     GNTPVDPHRKNDLILIDVDVERFQCEQKLRSFAHIENAGTDTVFSFVFQFTITNKQGV
                     PLNFTQTWNGIMLPGERIFFQLDEVNAEKGQSVVDYEIVKVNNVNDDGPLNNRVRKEI
                     FIISNLKQTVQLAGGQNVSACSGTQVEVVSGFIKPGRVDWFDALFGGKLIGSGNPFLA
                     PPAANNQILCMQAVYADFTGRTDIDEQIKENENKPYGGLRMTVHYPFILKSVKVYAAT
                     KGVRKIEMYQGNTLLFAKSITITKTGDTRLTLNFPVPAGKDIRLILAEDAKPLAYSLG
                     QSKFPYVIDNVVSIEGNIVKAQNAYCYFYDWQVEYNDFCGRIPVEIPFVHSTLQAGFV
                     VQDTNYIQKGIGANVVFTDTSINAQNRSWSFSNGQTSTALNPVINFDVPGEYIAKLTV
                     SNTATCFDTEIKKIHILPDTTTATTHFNLNNTFILYPNPNTGHFVIEIKNGSLISMGT
                     ELKIFSATGREMFNQKINSSEKEWFVKADKLAPGVYWAKITDTENTLVTKLIIE"
     gene            2429..2956
                     /locus_tag="PLE23_05965"
     CDS             2429..2956
                     /locus_tag="PLE23_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82846.1"
                     /translation="MTRFFYFFLLIMLVHNNVTAQKFEAPPNNQAEYEANYKKRILLD
                     TLAGIYIPKDTKDAFAQLNKKIDAKSKEKFIHTSPDTIATFAVKSLGSWMIRNWGFYE
                     GSRLGEFLRKKGVYHPEDQAAFLVIGYYRFLNNLPMEEEKRINEFQKKRKAAVDAKKR
                     KGEVISSEKIQKPKQ"
     gene            3163..5703
                     /gene="topA"
                     /locus_tag="PLE23_05970"
     CDS             3163..5703
                     /gene="topA"
                     /locus_tag="PLE23_05970"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HMX82847.1"
                     /translation="MVKNLLIVESPAKAKTIEKYLGKDFIVKSSYGHVRDLEKGSKGI
                     NIEKDFEPNYVVQPDKLKLVKELKEYASKSQEIWLATDEDREGEAISWHLAKVLGLDL
                     KSTKRIVFREITKQAIQNAIKEPRKVDINLVDAQQARRVLDRLVGFELSELLWKKIKG
                     QLSAGRVQSVAVKLVVDRERDIKEFNVTSFYKISAIFTVVDKNGKTTSLKAELPDNFA
                     SEKDAHTFLNKCSGADFTVNDIQVKPLTRKPSEPFTTSTLQQEASRKLGFSVQRTMIA
                     AQKLYEAGYISYMRTDSVNLSETAIAAIATEISSRYGSNYVQSRRYKSKNASAQEAHE
                     AIRPSYMDRTDVTNDRDQQRLYDLIWKRTIASQMADAKLEKTIVKIAISTIPNSHLVA
                     EGEVVTFDGFLKVYFESTDEEEDDEKGLLPPLTIGQILRLKKMDATERFTRPPARYTE
                     ASLVKKLEELGIGRPSTYAPTIGKIMEEKRGYVVKLTKEGSPRSYRLLTLENETIGTQ
                     TLTEMSGAAKNHLFPTDMGILVSDFLSEHFSGIMNYSFTADIEKKLDEVAEGDADWKK
                     IIRTFYGPFHDSVIKTEQNAERVSGERTLGIDPVSGRTVLVRMSKFGKPVVQIGSQSE
                     IAEGEKPAYANLSPGMSIDDVNLEEALELFKLPKSLGEYEGEEIVVASGRFGPFIKYG
                     TQNISLARGADPMALTLDEAMQLIEQKKQADAPVTEYQGMPVTAGKGRFGPFIKWNDM
                     YINIPKKYNPDTLTPSQVAELIEAKLDKESNRYVHNWPDLKLSIENGRWGPFIKFGKK
                     LFNLPKNAEGKRMTQEDAALLTLEDVKKLVTAEIPDAFEVKKKASKKA"
     gene            5779..7398
                     /locus_tag="PLE23_05975"
     CDS             5779..7398
                     /locus_tag="PLE23_05975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMX82848.1"
                     /translation="MQENHEITPAKNRIFMPLIIMTFLALGFVGGLGFKHFVPSFKST
                     HTAGGSDSTGSVDEILRFIQARYVDKISFEDLNEKTIRGLLSQLDPHSVYISKEELQS
                     VNDEMNGDFEGVGIEFMLVRDTISVVSTIFGGPSEKAGVLAGDQIIYVNDTLVSGKKL
                     DNSDIILKLRGRKGTKVKVGVKRPNKAGILSFTIIREAIPFNSIDAAMAIDDKTAYVK
                     INRFSAKTFQEFMGAADSLFLKGKKENLILDLRQNPGGYLQEATNLLSQLIADRNKLL
                     VYTKGRNFKKTEYETNGRSHFSVKKIAVLIDEGSASASEIVAGAIQDWDKGVIIGRRS
                     FGKGLVQEQYDLQTGGALRLTIAKYYTPSGRCIQKDFDHYSDEIEERYKDGELSGKLK
                     NNPKDSTKYFTSKGRVVYGSGGISPDIFIPIDTNLLSEQYFELRLAAAEFAIDEAKNF
                     RKAYPASLSVFLADKIGQNKLFEKFQQKQKLSKFKNLQQYDRKLKNYLNARLAKQLYG
                     QNAFFLIQNLEDEAVQKALMQFKATKLIPGL"
     gene            7443..8237
                     /locus_tag="PLE23_05980"
     CDS             7443..8237
                     /locus_tag="PLE23_05980"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HMX82849.1"
                     /translation="MKQYHDLLRYIFDHGVQKDDRTGTGTLSVFGYQMRFNLQDGFPL
                     LTTKKLHIKSIIYELLWFLKGDTNIKYLNEHGVRIWDEWADENGDLGPVYGKQWRSWE
                     TRDGKVIDQISDVIHQLQTNPNSRRIIVNAWNVGELQEMALSPCHCMFQFWVADGKLS
                     CQLYQRSADSFLGVPFNIASYALLTMMIAQVCGFKLGEFIHSFGDLHLYSNHVDQARL
                     QLEREFYPLPTMKINHEVKDIFDFKFDDFSLENYQFHPHIKAEVAI"
     gene            8404..>8937
                     /locus_tag="PLE23_05985"
     CDS             8404..>8937
                     /locus_tag="PLE23_05985"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMX82850.1"
                     /translation="MGKKYTYLQWSAVIMFLLSGMMLTAAPDANAGKDLFKANCAACH
                     NKNMKDDLTGPALGGVQERWAGREKLLHDWIRNSQAVIGSGDKYANDLYVKWNKSQMN
                     AFPNLTDDDINSLLLYIDGVFTGAIGGKPVAAVGATSAAPEQKSDSNILYWFIAGVLL
                     ILSFMLLKVVKSLNSLEN"
BASE COUNT         2892 a         1714 c         1905 g         2426 t
ORIGIN      
        1 gaacacaaga cacgtgcact gtttgtgcca caagaccaag cctggtttga tatcggtggt
       61 tctactacac ccaccaactg tggtgaccac ggttcgcact gtcttggtac tatattaggt
      121 ctcgatcgcc tcaataacga taccatcggt gtggcattca atgcacagtg gatgggtggt
      181 aaaatactct gcggtggagg cacttacggt attctgcagg cattgcagtg ggcaataaac
      241 cctgacggca acaaaaatac tacagttgat atgccggatg tcatcaacaa ctcctggtac
      301 gacccaggag tagctaatga ctgcgaaagc ggatatgtag atgtagaggc cgcactcgaa
      361 gcagcaggta ttgcttcaat tttttcggca ggcaatgccg ggcccgatga agcgaccatt
      421 actccacctc ataatgtcaa tataagcgtg gtcaatagct ttacaatagg tgctgtcaat
      481 ggtaatgttt catccttacc gatagccgat ttttcttcac gaggcccatc aaaatgcggt
      541 ggtacaggct cagttttgat aaaaccggaa gtgtcggctc ctggtgtgtc tgttcgctct
      601 tgtacgtttg acaaagctta tgatttaaaa tcgggtacat ctatggctgc accgcatacc
      661 tgtggtgcta ttttattgtt gaaggaagcc ttcccttatc tgtcaggtta cgatttcaag
      721 atggcattgt acaatacatg tacagacctt ggtgtaccgg gcgaagataa cgtttatggc
      781 caaggtatca tcaacgtttg ggcagcctac aattatctta ttgctcaagg caatacacct
      841 gttgacccac accgaaagaa cgacctgata ttgatagatg tagatgtaga gagatttcaa
      901 tgtgaacaaa agcttaggag tttcgcacac atcgaaaatg ccggaactga tacggtcttc
      961 agttttgtgt ttcaatttac cattaccaat aagcaaggag tacctcttaa ttttactcaa
     1021 acctggaatg gaattatgct accgggagag cggatattct tccagcttga tgaagtaaat
     1081 gccgaaaaag gacagtctgt cgtagattac gaaatagtaa aggtgaataa tgtcaatgac
     1141 gatggtccac tcaataacag ggtaaggaaa gaaatattca tcatcagcaa tctcaagcaa
     1201 actgtacaac ttgccggcgg acaaaatgtt tctgcatgtt cgggtactca ggtagaggtg
     1261 gtttcgggtt ttattaaacc gggtagggta gattggttcg atgcactttt tggtggtaaa
     1321 ttgatcggaa gtggcaatcc attcctcgca ccacctgctg ccaataatca gatattgtgt
     1381 atgcaagctg tttatgcaga ctttacaggc agaacggaca tcgacgaaca aataaaagaa
     1441 aatgaaaaca aaccctatgg cggactaaga atgacggttc attacccgtt tatcctaaag
     1501 tctgtcaagg tatatgctgc taccaaaggt gttcgtaaga tagagatgta tcagggcaat
     1561 actcttttgt ttgccaaatc cattaccatc acaaagaccg gagatacaag gttgacgctt
     1621 aacttcccgg taccagcggg aaaagatata cgtttgatac tcgccgagga tgccaagccg
     1681 cttgcttatt cacttgggca gtcaaagttt ccttatgtga tagataatgt tgtaagtatt
     1741 gagggcaata ttgtcaaggc ccaaaatgca tactgctatt tctatgactg gcaggttgag
     1801 tacaatgatt tttgcgggcg tattcccgta gaaatacctt ttgttcactc aacattacag
     1861 gcgggttttg tagtgcagga tacaaattat atacagaaag gcatcggagc caatgttgtg
     1921 tttacagata cttcaatcaa tgcccaaaac agaagctggt cattcagcaa cggacagacg
     1981 agtacagcgc ttaacccggt tatcaacttt gatgtcccgg gtgagtatat cgcaaaatta
     2041 accgtttcga atacggccac ttgcttcgat accgaaatta aaaaaattca cattctgccg
     2101 gatacgacca ctgcgacaac tcattttaac ttgaataata cattcatact ctacccgaac
     2161 ccgaatacag gacattttgt tatagaaata aaaaatggaa gtttgatatc aatgggcacc
     2221 gagttgaaaa tattttcagc caccggccgg gaaatgttta accaaaaaat taattcctcc
     2281 gaaaaggagt ggtttgtgaa agcagacaaa ctggctcccg gtgtttattg ggccaaaata
     2341 acggatacag aaaatacact tgtaacaaaa ttaattattg aataaaacgt ttttctgctt
     2401 gaatatattt gtagtccaat tcaaaaaaat gacacgattc ttttattttt ttctgctcat
     2461 aatgcttgta cataataatg taactgcgca gaagtttgag gcaccaccca ataatcaggc
     2521 agagtatgag gccaattata aaaaaaggat attactagat acgctggcag gtatttatat
     2581 acccaaagat acaaaagatg ccttcgctca gctcaataaa aaaattgatg ccaaatcaaa
     2641 ggaaaaattc atccatactt caccggatac cattgctaca tttgccgtaa aatcattagg
     2701 ctcatggatg attcgcaatt ggggttttta tgaaggctct cgtttgggtg agtttttgcg
     2761 caaaaaggga gtttatcatc ccgaagatca ggcagctttt ttggttatcg gctactatcg
     2821 ttttttaaat aatcttccaa tggaggaaga aaagcggatc aacgaatttc aaaaaaagag
     2881 aaaggcagca gtagatgcca aaaaacgcaa gggtgaagta atttcaagtg aaaaaattca
     2941 aaaacctaag cagtaaagtc atcttattct ttatcaattt agtctttttc ttcatcgttg
     3001 aaatgattta tttgtaggca aaatttttat ctgctcattc attgtacata accggtaaca
     3061 ctactttttt cttacaaata taaattttag gtgaaacttt gtggaaaatg gatatacttt
     3121 tgcgtcataa ttcataccaa gttcatctaa aatcaaacag gaatggtaaa aaacctgctc
     3181 atcgtagagt cgccggcaaa agcaaaaaca atagagaaat acctcggtaa agactttatc
     3241 gtcaaatcga gctacggaca cgtccgcgac ctggagaaag gaagcaaagg tatcaatatc
     3301 gaaaaggatt tcgaacctaa ctacgtggtt caaccggata aacttaagct cgtaaaagaa
     3361 ctgaaggagt atgcttcaaa atcacaggaa atttggcttg caacggacga agaccgcgaa
     3421 ggagaagcta tttcatggca tcttgccaaa gttttaggac ttgatctgaa gagtactaaa
     3481 cggatagttt tccgcgaaat taccaaacaa gcaatacaga atgctattaa agaaccaaga
     3541 aaggttgaca ttaatctcgt tgacgctcaa caagcaagaa gggtactcga ccgcctcgta
     3601 ggttttgaat tatctgaact gctctggaaa aaaatcaaag gacaactatc tgctggtcgt
     3661 gtacagtcgg ttgctgtaaa acttgtagtg gatcgtgaac gcgatataaa ggaatttaac
     3721 gtaacttcat tttataagat ttccgcaata tttacggtag ttgataaaaa tggcaagact
     3781 acaagtttga aggccgaact acctgataat tttgcaagcg aaaaagacgc tcatacattt
     3841 ctcaataaat gctccggtgc cgatttcaca gtaaatgaca tacaggtaaa accacttacg
     3901 cgcaaaccat ctgagccatt tacaacatcc acactacaac aagaggcgag ccgcaaactt
     3961 ggcttctcag tccagcgtac catgattgcc gctcagaaat tatacgaagc cggttatatt
     4021 tcttacatgc gaaccgactc ggtcaacctg agcgaaacgg ctattgcagc tatagcaaca
     4081 gagatttctt caaggtatgg atcaaactat gtccagtcgc gaagatataa gagtaaaaat
     4141 gcgtcggctc aggaagcaca tgaggccatc cgcccatcct acatggatag aacagatgtg
     4201 acaaatgatc gtgaccaaca acgactgtat gaccttatct ggaaaaggac catcgcttcg
     4261 cagatggcag atgcaaaact tgaaaaaacc atcgtaaaaa tagccatctc aactatcccg
     4321 aatagtcacc tggtggcaga aggcgaagta gttacctttg atggtttcct gaaagtatat
     4381 ttcgaatcaa cagacgaaga ggaagatgat gaaaaaggat tattgcctcc tttgacaata
     4441 ggtcagatcc tccggcttaa gaaaatggat gctacggaaa ggtttacaag accacccgca
     4501 cgttatacag aggctagcct tgtcaaaaaa cttgaagaac ttggcatcgg ccgaccttct
     4561 acttatgcac ctaccattgg caaaataatg gaagaaaaac gcgggtatgt agtgaagtta
     4621 acgaaagaag gctcaccccg aagctacaga ttgcttacac tcgaaaatga aacaatcggc
     4681 actcaaacac ttactgagat gtcaggtgct gctaaaaatc accttttccc gactgatatg
     4741 ggcattttgg tatccgattt tctcagcgaa catttcagtg gcatcatgaa ttacagcttc
     4801 accgccgaca tcgagaaaaa actggacgaa gtggcagaag gcgacgctga ctggaaaaaa
     4861 atcatcagga cgttctatgg gccgtttcat gattctgtaa taaagactga acagaatgcc
     4921 gaaagagttt cgggtgaaag aacgctaggt attgacccgg tatccggcag aacagtatta
     4981 gtgcgaatgt ccaaatttgg taaaccggtg gtacagattg gaagccagag cgaaattgcc
     5041 gaaggcgaaa aaccagctta tgctaatctg tctccgggaa tgtctatcga tgatgtaaat
     5101 cttgaggaag cgcttgaatt attcaaattg cccaaatcac tcggtgaata cgagggcgaa
     5161 gaaatagtgg tggcatcagg gcgttttggt cccttcatta aatacggaac tcaaaatatc
     5221 agcctggcaa gaggtgcaga tccaatggca ttgacacttg atgaagcaat gcaactcatc
     5281 gaacaaaaaa aacaggcaga tgcaccagtt actgaatacc agggtatgcc ggtgacagca
     5341 ggaaaaggaa gattcggtcc tttcataaaa tggaatgaca tgtacatcaa tatacctaaa
     5401 aagtataatc cggatacact tactccttct caagtggcag aattaataga agccaagctt
     5461 gacaaggaaa gtaaccgtta tgtacataat tggcctgacc ttaaactttc aatagaaaat
     5521 ggacgttggg ggccttttat aaaattcggt aaaaaattgt tcaatctgcc aaagaatgcc
     5581 gaaggaaaac gcatgactca ggaagacgca gcattattaa cactcgaaga tgtaaagaag
     5641 cttgtcaccg ccgaaattcc ggatgcattt gaagtaaaga aaaaagcaag taagaaagct
     5701 taaaaacaaa ttttcccggc aaacaaccaa ttgagtaaat tgagtatttg cttaactttg
     5761 ctaaataaac gaacctgaat gcaagaaaac cacgaaataa caccagccaa aaataggata
     5821 ttcatgcctt tgatcatcat gacattcctt gccttagggt ttgtaggcgg acttggcttt
     5881 aaacatttcg tgccgtcctt taagtcaaca catacagcag gtgggagtga ctctacagga
     5941 agtgtagatg aaatacttcg gtttattcag gcaaggtacg tcgataaaat ttcatttgaa
     6001 gacctcaatg aaaagactat acgtggatta ctgtcccaac ttgacccgca ttccgtttat
     6061 atttcaaagg aagagttaca gtcagtcaat gacgagatga atggcgactt tgaaggtgtt
     6121 ggtattgaat ttatgcttgt gcgcgatact attagtgtgg tatctacaat ttttggaggg
     6181 ccgtcagaaa aagccggtgt tcttgcaggt gatcagataa tatatgtcaa cgatacgctt
     6241 gtttccggca aaaaactcga taattctgat ataattttga agctgcgtgg caggaaaggt
     6301 acaaaagtaa aagtaggcgt caagcggcct aataaggcag gcatcttgag ttttaccatt
     6361 atacgtgagg ccattccgtt caatagtatt gatgccgcaa tggcaatcga tgataaaacc
     6421 gcttatgtca aaataaacag attttctgcc aaaacattcc aggaattcat gggagcagcc
     6481 gattctctgt ttttgaaagg aaaaaaagaa aaccttatcc ttgatctcag gcagaatccc
     6541 ggtggatatt tgcaggaagc aaccaacttg ttgtcccaac tcattgccga cagaaataag
     6601 cttctggtat ataccaaagg tagaaatttt aagaaaacag agtacgaaac aaatggcaga
     6661 agccacttta gcgtaaaaaa aattgccgtg ttaattgatg agggctctgc atccgccagc
     6721 gaaatcgtag caggtgccat ccaggactgg gataagggag tcatcatagg aagacgctca
     6781 ttcggaaaag gtttggtaca agaacaatac gatctgcaaa ccggcggcgc actccgactt
     6841 actattgcca aatattatac gccatctggt cgctgtatcc agaaagattt cgatcattac
     6901 agtgatgaaa ttgaagaacg ctataaagat ggagaactct ccggcaaact aaagaataat
     6961 cctaaagaca gcaccaaata ttttacttca aaaggtagag tcgtgtatgg ttcgggagga
     7021 ataagtccgg atatcttcat cccgatagac accaacctgc tttctgagca atattttgag
     7081 ttaagactgg cagcagcaga atttgcaatt gatgaagcta agaattttcg aaaagcttac
     7141 cctgcaagtt tgtctgtttt ccttgccgat aaaataggcc agaataaact tttcgaaaaa
     7201 tttcaacaaa aacaaaaact tagcaaattt aagaatctac agcaatatga caggaaacta
     7261 aaaaactatt taaatgcacg ccttgccaaa caactttatg gtcagaatgc cttctttttg
     7321 atccaaaatc ttgaagatga agctgtgcaa aaagccttaa tgcaatttaa agcaactaaa
     7381 ttgattcctg gtttgtaaat ttgttcttat atttctgcca cattcatact aaaaaccata
     7441 acatgaaaca atatcatgat ctcctaaggt atattttcga ccacggagtt cagaaagatg
     7501 atagaacagg caccggcacc ctaagtgttt tcgggtatca gatgaggttt aatcttcagg
     7561 atggtttccc gttgcttact accaaaaaac tccacatcaa atccattatt tatgagttgt
     7621 tatggttctt aaaaggtgat accaatatta aatatttaaa tgaacacggt gtacgtatct
     7681 gggatgagtg ggcagacgaa aatggcgacc tcgggccggt gtatggtaaa caatggcgct
     7741 catgggaaac ccgcgatgga aaagtgatag atcaaatatc agatgtcatt caccagctcc
     7801 agaccaatcc caactcaaga cgtatcattg tgaatgcctg gaatgttggc gaattacaag
     7861 agatggcatt gtcaccatgt cattgtatgt ttcagttttg ggttgcagat ggtaagctca
     7921 gttgtcagtt gtatcaacgt tctgccgata gttttttagg tgtaccattt aatattgcct
     7981 cttatgcttt gctcacaatg atgattgcgc aggtttgtgg tttcaagctg ggcgaattta
     8041 tacatagctt tggtgatttg cacctctaca gtaatcatgt tgatcaggca agattacaat
     8101 tggaaagaga attttatccg ctgccaacca tgaagattaa tcatgaagta aaagatatat
     8161 ttgattttaa gtttgatgat ttttcattgg aaaattatca atttcaccca catatcaagg
     8221 cagaagtcgc tatttgatgt gttaagaatt tcttaatttt atttagaaat gttctaaata
     8281 agcagaagaa tgataatata ttttggttaa aattttagga aatgtatttt tgcgttgaca
     8341 attgtatgac aatgtctaaa agattaagtt tttagttctt ataagttgaa aatttttcag
     8401 gaaatgggca aaaaatatac gtatttacaa tggtctgctg tcatcatgtt tttgctgtcc
     8461 ggtatgatgc tgaccgctgc accggatgcc aatgctggca aggacttatt caaagctaac
     8521 tgtgccgctt gtcacaataa aaatatgaag gatgacctga ccgggcctgc tttgggtggt
     8581 gttcaggaaa gatgggcagg cagggaaaag ttgcttcatg attggattag aaattctcag
     8641 gctgtgattg gttcaggaga taaatatgcc aatgaccttt atgtcaaatg gaataaatct
     8701 caaatgaatg cattccctaa cctgacggac gatgacatca attctttgct tttgtatatt
     8761 gatggtgttt tcaccggagc aataggtggt aagcccgtag ctgcagttgg tgctacctca
     8821 gcagcgcctg agcaaaaatc tgacagcaac atcctttatt ggtttattgc gggtgtattg
     8881 ctcattttgt cgtttatgtt attaaaagtt gttaaatctt tgaattccct agaaaat
//
LOCUS       DAOQQY010000093         8934 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2988_length_8934_cov_6.390472, whole genome shotgun sequence.
ACCESSION   DAOQQY010000093 DAOQQY010000000
VERSION     DAOQQY010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2988_length_8934_cov_6.390472"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PLE23_05990"
     CDS             <1..208
                     /locus_tag="PLE23_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82851.1"
                     /translation="MMAIDLGSNTAVMKCIQYCLEHGLITDWFLFNDASLRLCPPLII
                     NEEDIRRACKILLDAFDYYLQVPE"
     gene            complement(235..1791)
                     /locus_tag="PLE23_05995"
     CDS             complement(235..1791)
                     /locus_tag="PLE23_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HMX82852.1"
                     /translation="MLIKTYSSAVHGVDAQTITVEVNAGGNVGAGKIAYFLVGLPDNA
                     VKESNHRIEAALKNTGFTMPRMKIVINLAPADIRKEGSAYDLPIAMGILASSKQIEVP
                     DIDQYVMLGELSLDGGLRPVKGALPIAIQARKEKFKAIILPKDNAREAAIVNDLDVYG
                     VESLKEAVELLQGISDIRPVTVDTRAEFADRQNIYEIDFTDVRGQENIKRALELAAAG
                     GHNAILIGPPGAGKTMLAKRLPTILPPLTLHEALETTKIHSVSGTLPVNSSLVTTRPF
                     RSPHHTISDVAMVGGGSNPLPGEISIAHNGVLFLDELPEFKRTVLEVMRQPMEERKVT
                     ISRAKSTVDYPASFMLIASMNPCPCGYHNHPEKECVCAPGVVKKYISKISGPLLDRID
                     LHVEVTPVSYDELSSFERPRESSENIRERVIKAREIQMERFKNHPEIYSNAQMPAQMA
                     REVCQITTAGQTLLKTAMTKLQLSARAYDRILKVARTSADLAGSADIKIEHLAEAIQY
                     RSLDREGWAG"
     gene            complement(1889..2683)
                     /locus_tag="PLE23_06000"
     CDS             complement(1889..2683)
                     /locus_tag="PLE23_06000"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HMX82853.1"
                     /translation="MKIQTIPFILLLALSACKSDTEQTTKPNSNSVLSFTDEKVFTHT
                     GNCDDEKQPCALVDMKFLQMTDSSQQCYADLNQTLKLSAMQCAMLLLDSVKGQPEINK
                     MAAEYIKSYEDYIKEFPDVAMSWEARSTMKLLFQNDSLLSFLTTGFNFSGGAHPNSAN
                     EIIVYDKARCKKLSTNELFTENDNLLKTVEKHFRAKREVPANQSLNDFGFFFENDKFI
                     LPANIGFEKDSVLFIYNDYEVGPHVIGGTEFKIPKSELLPFLKTKY"
     gene            complement(3076..3534)
                     /gene="ybeY"
                     /locus_tag="PLE23_06005"
     CDS             complement(3076..3534)
                     /gene="ybeY"
                     /locus_tag="PLE23_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMX82854.1"
                     /translation="MTEPSFPFELEHLIEFFTEDTSYDLNKEDEVIQWLKDIIEAEKK
                     GLDAITYIFCSDDYLLSINETYLKHDDYTDIITFPYNADPIEGDVFISVERAKENAAK
                     FNVSETEEIHRLLVHGLLHLLGYDDKSPDSKTKMTELEDHYLAKRTFVQV"
     gene            complement(3531..3935)
                     /locus_tag="PLE23_06010"
     CDS             complement(3531..3935)
                     /locus_tag="PLE23_06010"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMX82855.1"
                     /translation="MKEALHMVQISSQPNNIALVEALVQKITTGKNLSQDLHGNILVS
                     LTEAVNNAIIHGNCCDKNKNVKIEYTERRNLIKFKVSDEGKGFNPDSIPDPTAPENIY
                     NIGGRGVFLMRQLCDQIVFKDKGRTVEMHFNI"
     gene            complement(4065..7412)
                     /locus_tag="PLE23_06015"
     CDS             complement(4065..7412)
                     /locus_tag="PLE23_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4175 domain-containing protein"
                     /protein_id="HMX82856.1"
                     /translation="MAEFNDNYTLLIQKLDHFIRKFYTNQLLKGTLYSAGVVLGLFLL
                     INLLEYNFYFHTTARKLVFFSFILISIAAISKWVVLPAMHYFRLGNIINHEQAADIIG
                     NHFTDVKDKLLNILQLKKQSYNAVETALINASINQKSEEIKPVPFKAAIDLKENKKYL
                     KYALPPLLLLLGVMLFDSSIIRDSTYRLINNNKEFVKPAPFAFILKNDQLKVGQNEDY
                     ALQITTQGSVIPNEVFVEIGGHEYRLKKEAHNVFSYVFSNCQKDTEFRLLSGDVSSAT
                     YQLSVLLKPNIKSFVVTLNYPDYTGRQDETVTNVGDVLVPAGTKILWNFSTDNTSRLS
                     YKFGNESKTVAATKRGESEYSFSLRAMNNMPYTVFFRNELLPNADSVNYNLSVIPDAY
                     PVISVQRFDDSTNRRLVYFVGDVSDDYGLRNLTFNYKIKKYKAAEGPLNTERLQAPQG
                     INGVFNYSWDLRKLNLNPGDEINYYFEVYDNDAVNGSKPARTSMMSYKMPTVDELEQL
                     TSQNSEEIKKDLEKSLRESKKIEDELQKIREKLLQKKEIDWQTRKELEKLLERQKQVE
                     KQIQQAKQKFEENLQNQNETSEPKEDVEQKQEQLKSMFDDVMQDKMKDLLQQIQDLLQ
                     QMDKDQTLQKMDEMKKEQKQSSQTMERLKELYKQLEVEKNIQDIVNKLEELSEKQEKL
                     SDQTEKNEKSQDQLQKEQSEMQKEFEKLQQKMDETDKQNKQLDKPKDFDQQKEKSDDV
                     KDSMLKSQEDMKQGANSKASQKQKKAARKMKEMAQSMSEQAASGDQEQAEEDYNAMRQ
                     LMENLISLSFDEEETMTQFVKTPINTPKYTELGQRQKRIKDDFKMVEDSLFALSKRVS
                     QIETFILEKVAEVNLNMDNSLSNIQERYKPQAQDFQQRTMKNLNDLALMLDEAMNNMQ
                     QQMSNKSGSKNCKKPGGKSSSSGSDRKPQDKMSKSQKGINEDMKKMMEGTKNGEGQPK
                     SKEFAEMAARQAALRKMIENMKKQNQEQGKGAGENKILDELIQDMNKTEKDLVNKRLN
                     NEMLKRQKEIETKLLESERAEREREFDNKRKAEQADQIDNRMPPALKEYIKKRQSEIE
                     LYKDVNPSLKIYYKNLVEEYFKTLKSS"
     gene            complement(7562..>8934)
                     /locus_tag="PLE23_06020"
     CDS             complement(7562..>8934)
                     /locus_tag="PLE23_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMX82857.1"
                     /translation="MASAQYNVHPLIHYHYNEKTDMFDAHSYNKGGLVLHMLRNMLGD
                     KVFFKALKNYLAKNAFTAVEVHNLRMAFEDASGQDLNWFFDQWYNASGHPKLEYYKSY
                     DETSEEVVIDVIQTQSQADVPKVFRFPLAVYITDQSGNKVKYERWVNERNQQYRFKVP
                     AKPLLVEIDPEKIILCERTDYGEDTILTHLYNPTSPALARYDAIDKIAQAIDGGQDEY
                     RTVMGIAINDPLEAIRAAAVVRCDPNQAGIMDKIKALANNDPKEEVRSAATSFIGENN
                     PELFRSMANELLTKGTEAQKARMLLLVHQFLPEKAMEYAEKFEKNASEAMLTSLAKLY
                     ADAGKTEKLSIIENGMSKLDDYTLISYTGNYATLLAKSDAAKARTGFDKLYAQGMNAE
                     SKIKRFAAVLGFFQVYVLDAMAKQELEPTGFNIDMDYIKSKMGEMISNEKDPELQNAY
                     KRFKLE"
BASE COUNT         2205 a         1815 c         1720 g         3194 t
ORIGIN      
        1 gatgatggcc attgaccttg gaagcaacac cgctgttatg aagtgcatac aatattgtct
       61 tgaacacggc cttattacag actggttttt gtttaatgat gcttcacttc gactgtgtcc
      121 gccactaatc atcaatgaag aggatatcag acgtgcatgt aaaattctgc ttgacgcttt
      181 cgattattat ttacaagtac cagaataaca taccgtgctt ccttaaagta caggttaacc
      241 agcccagcct tcgcggtcca ggcttcggta ttgaatggct tcagccagat gctcgatttt
      301 tatatcagca ctaccagcaa gatccgcaga agtacgggct actttcaata tcctgtcata
      361 ggcccgggca gacagttgga gtttggtcat ggctgtcttc agtaatgttt gtccggctgt
      421 tgttatctgg caaacttctc ttgccatctg tgccggcatt tgagcattgg agtatatttc
      481 aggatggttt ttgaaacgtt ccatttgaat ttctctagcc tttattacgc gttcccggat
      541 gttttcactg ctttcccttg gcctttcaaa gctacttagt tcatcataac tgacaggagt
      601 aacttccaca tgcagatcga ttctgtcaag caaagggcct gaaattttac tgatatattt
      661 tttcactaca cccggtgcgc agacacattc cttttcggga tggttatgat aaccacaggg
      721 gcatgggttc atagaagcaa tgagcataaa actagcagga taatcaaccg ttgatttggc
      781 tcttgaaatg gtcaccttgc gttcttccat cggttgtctc atcacttcaa gtaccgtgcg
      841 tttgaattcc gggagctcat caagaaagag tacaccgttg tgtgctatac ttatttcacc
      901 gggcaaagga tttgaaccgc cacctaccat agctacatca ctaatggtat ggtgcggaga
      961 ccgaaacgga cgtgtggtta ccaatgaaga attgactggc aaagtacctg atactgagtg
     1021 tatttttgtt gtttcaagcg cctcatggag tgtcagtggt ggcaatatag taggcagtct
     1081 tttagccagc attgtcttac cggcacctgg tggtccaata agaatggcat tatgccctcc
     1141 ggcagcagca agttctaatg ctcgcttgat gttttcctgg cctcggacat ctgtaaaatc
     1201 tatttcatag atattttgcc tgtctgcaaa ttcggctcgg gtgtcaacgg tcactggcct
     1261 tatatcggaa atgccttgta aaagttcaac tgcttctttt aatgattcga caccatacac
     1321 atccaagtca ttgactatgg ctgcttctcg tgcattgtct ttgggtagaa taatcgcttt
     1381 gaatttttct ttacgtgcct gtatagcaat aggtagcgca ccttttacag gcctgagtcc
     1441 accatccaaa gatagctctc cgagcataac gtattgatct atgtccggca cttctatctg
     1501 tttagaactg gccagaatac ccatcgctat cggtaggtcg taagctgagc cttccttgcg
     1561 aatatcggcc ggtgccaagt tgataactat tttcattctg ggcattgtaa aacccgtatt
     1621 cttcagagcg gcttctattc tatggttact ttccttaacc gcattatccg gcagccctac
     1681 cagaaaatag gctattttac cggctccaac attgccaccc gcattgacct ctaccgttat
     1741 tgtctgcgca tctacaccat gcactgcact tgaataagtc tttatcagca tctttccctt
     1801 ctttgattaa ctaatattca gtacaatgac gcatcaactg cacaaatggc ataaattttt
     1861 aacaaaattt ttttaaaaaa tctgtagatt aatatttggt cttcaaaaat ggtagtaact
     1921 ccgatttcgg aattttgaat tcggtaccac cgattacgtg cggaccaact tcgtaatcat
     1981 tatatataaa caatacactg tctttttcaa atcctatgtt tgcaggaagt ataaatttgt
     2041 cattttcgaa gaaaaaaccg aaatcattca ggctttggtt agcaggcact tctctttttg
     2101 ctctgaaatg tttttctact gtcttcaaca aattatcatt ttctgtaaat aactcgttgg
     2161 ttgagagttt cttacacctg gccttgtcat agacgattat ttcattagct gaattgggat
     2221 gcgcaccacc actaaagtta aaccctgtag taaggaagct gagcaaactg tcattctgaa
     2281 acaataattt catcgtgctt cttgcttccc aactcattgc aacatccgga aactctttaa
     2341 tatagtcttc gtaacttttg atatactctg ccgccatctt gtttatctcc ggctgccctt
     2401 taacagaatc gagcaataac atggcacatt gcatggccga caatttcaga gtttggttca
     2461 ggtctgcata gcattgctgg ctcgaatcag tcatctgaag gaatttcata tcaacaagtg
     2521 cacaaggctg tttttcatca tcacagttgc cggtgtgtgt aaatactttt tcatctgtaa
     2581 agcttaagac agagttgctg ttaggctttg tcgtttgctc ggtgtctgat ttacatgcac
     2641 tcagtgccaa tagtaggatg aaggggattg tttggatctt cattttttat aatatttgat
     2701 aaatgagtat ggttttctgt tgttaaggta attgagatta tcatcgttac agtaagcttc
     2761 ttgttcaaaa gatatggcct tatatgcttc gaatggtttt ttagtgaagc gatggaaaaa
     2821 atattcgatg aaataaagaa caaaaaaaat tatgaataag gtttctatct gctgcctaat
     2881 atgaatcctt tcatggttta gtagctgtgc tttgttttta atttgcttgc ctacaaaaat
     2941 aaacggaaat atggtaagac cggaagcttt tcctaaactc agcaacttta gccaaaaatc
     3001 actccgtatc aggaaccctt taaataaaaa ccttggcagc atctgttgtg tgaattaaat
     3061 gcaaaggtat aacaatcaga cctgaacaaa cgttctttta gcgaggtaat gatcttccag
     3121 ctctgtcatt ttcgtttttg agtcgggaga tttgtcatca taacctaaga ggtgcagcag
     3181 cccgtgcacc aacaaacggt gaatttcttc tgtttcgctt acattaaatt tcgctgcatt
     3241 ttctttagcc cgttctacac ttataaacac atcaccttct attgggtctg cattgtaagg
     3301 aaaggtaatg atgtctgtat aatcgtcgtg tttgaggtat gtttcattga tgctcagcag
     3361 gtaatcatca ctgcaaaaga tatacgtaat ggcatctaga cctttttttt ctgcttcaat
     3421 aatgtccttt agccattgga tgacttcatc ttccttgttg aggtcatagc tggtatcttc
     3481 ggtaaaaaac tctatcagat gttctagctc gaaaggaaat gatggttctg tcatatattg
     3541 aagtgcattt cgactgttct gcctttgtct ttgaatacga tttggtcaca gagttggcgc
     3601 ataaggaata ctcctcttcc gcctatgtta taaatatttt caggtgctgt cgggtccgga
     3661 atactatccg gattaaagcc ttttccttcg tcagacactt tgaactttat taggttgcgt
     3721 ctttctgtat attcgatttt tacattttta tttttgtcac aacaattgcc gtgaatgatg
     3781 gcattgttaa cagcttcggt aaggcttaca agaatattgc cgtgcaaatc ctgagagaga
     3841 tttttacctg ttgtgatttt ctgtacgagt gcctcaacaa gtgcaatgtt gttgggttga
     3901 gatgaaattt gaaccatgtg caaagcttct ttcattatcc atgagtttgg taaataaaaa
     3961 gtttgtttcc gacagaacga atctgtgaaa tgctcatgga tttcaccata caggcgtata
     4021 tcctgatggt gtatttttca aactttatgc aattaaaatc ttttttacga acttttcaag
     4081 gttttgaagt attcttccac taaattttta taataaattt tcaaagaagg gtttacatcc
     4141 ttgtaaagtt cgatttctga ttgtcttttc tttatatatt ctttgagtgc cggtggcatt
     4201 ctattgtcta tttgatctgc ttgttctgct ttgcgtttgt tgtcgaattc acgctctcgt
     4261 tctgctcttt cagactcaag aagtttggtt tcgatttcct tttgacgctt aagcatttcg
     4321 ttattgaggc gtttattgac cagatctttc tctgtcttgt tcatatcttg aatgagctca
     4381 tccagtattt tattttctcc tgcgcctttt ccttgttcct ggttttgctt cttcatattt
     4441 tctatcattt tacgcagtgc agcttgtctg gctgccatct cggcaaattc cttacttttg
     4501 ggctggcctt ctccattctt agtaccttcc atcattttct tcatatcctc gttgatacct
     4561 ttctggcttt tgctcatttt gtcctgaggt tttcggtcag accctgacga agacgatttg
     4621 ccacctggtt ttttacagtt tttactgcct gacttgttag acatctgttg ttgcatgttg
     4681 ttcattgctt catcaagcat gagtgccagg tcattgagat ttttcattgt acgctgttga
     4741 aaatcctgcg cctgcggttt atatctttcc tgtatattag agagagagtt atccatattg
     4801 aggttaactt cagctacttt ttcaaggatg aatgtctcta tctgtgacac gcgtttactt
     4861 aacgcaaaca gactgtcttc taccatttta aaatcatctt tgatgcgttt ttgtcgttgg
     4921 ccgagctctg tatatttggg cgtattgata ggtgttttta caaattgtgt catggtctct
     4981 tcttcatcaa atgacaggct tatgagattt tccatcagtt ggcgcatggc attatagtct
     5041 tcttctgcct gttcctggtc gcctgaggct gcttgttcag acatagactg tgccatttcc
     5101 ttcatctttc gggcggcttt tttctgtttt tgacttgcct tgctgttggc tccttgcttc
     5161 atatcttcct gactcttcag catggagtct tttacgtcat ctgatttttc tttttgctgg
     5221 tcaaaatcct ttggtttatc gagttgttta ttttgtttgt ccgtttcgtc cattttttgt
     5281 tgcagttttt cgaattcctt ctgcatctct gattgctctt tctgaagttg atcctgcgat
     5341 ttttcgtttt tctcggtttg gtcgcttaat ttttcctgtt tttctgacag ttcttcgagc
     5401 ttattgacga tatcctgaat atttttttct acttcgagtt gcttgtacag ctctttgagt
     5461 ctttccattg tctgtgaaga ctgcttttgt tctttcttca tttcatccat cttttgcagt
     5521 gtttgatctt tatccatttg ctggaggaga tcctgtatct gctgaaggag atctttcatt
     5581 ttgtcctgca ttacgtcatc aaacatactt ttgagttgtt cttgtttttg ctcaacatcc
     5641 tctttgggtt ctgatgtttc attttggttt tgcagatttt cctcaaattt ttgttttgcc
     5701 tgctgtattt gtttttctac ctgcttttgc ctttccaaca atttttcaag ttcttttctt
     5761 gtctgccaat ctatttcctt tttctgtagc aatttttctc tgatcttctg tagttcgtcc
     5821 tctattttct tcgactcacg taatgatttt tcgagatctt tctttatttc ctcgctgttt
     5881 tgggatgtta actgctccag ctcatctact gtaggcattt tataagacat catggatgtg
     5941 cgtgcaggtt tgcttccgtt tacagcatca ttatcataga cttcgaagta atagtttatt
     6001 tcatcgcccg ggttaagatt gagtttgcgc aggtcccatg aataattaaa tacaccattg
     6061 ataccctgtg gtgcctgtag cctttcggta tttagcgggc cttctgctgc cttgtatttt
     6121 tttattttat aattgaatgt aaggtttctt agaccataat catcagatac atcgcctaca
     6181 aaatatacaa gacgtctgtt ggtgctgtca tcaaaacgct gtaccgaaat gacaggatag
     6241 gcatccggaa ttactgacaa gttgtaattg accgaatcgg cattgggcaa taattcattc
     6301 ctaaagaaaa cagtataagg catgttgttc attgcccgca ggctaaaact atattctgac
     6361 tcgccacgtt tggttgcagc tacagttttt gattcatttc caaatttata gctaagccta
     6421 cttgtattgt ctgtactaaa attccaaagt atttttgtac ctgccggtac caacacatcg
     6481 cctacattag taaccgtttc atcctgtctg ccagtatagt caggataatt gagcgttacg
     6541 acaaaacttt taatattggg tttgagcaaa acgctaagtt gatatgtagc agacgaaaca
     6601 tcacctgaca acagcctaaa ctcggtatct ttctgacaat tgctgaacac gtagctgaat
     6661 acattgtgtg cttctttctt aagtctgtat tcatgaccac ctatttctac gaacacttca
     6721 ttcggtataa cagagccttg agtagtaatc tgtagtgcgt agtcttcatt ctggccaact
     6781 ttgagctgat catttttaag tataaaagca aaaggagccg gttttacgaa ttctttattg
     6841 ttattgataa gcctgtaggt actatctctg atgatacttg aatcaaaaag catgacaccc
     6901 agtaaaagga gtagtggcgg taaagcgtat ttgaggtatt ttttgttttc ctttaggtcg
     6961 atagctgcct taaaagggac aggttttatt tcttcagact tttgattaat agatgcattg
     7021 ataagtgcag tttcgacagc attatacgat tgttttttta actggagtat attgagcaat
     7081 ttgtctttca catctgtgaa atgattgcca atgatgtcgg cagcttgctc gtgattgatg
     7141 atattaccaa ggcggaaata atgcatagcc ggcaacacaa cccatttgct tatggctgct
     7201 attgaaataa ggataaatga aaaaaataca agcttacggg cagtagtatg aaaatagaaa
     7261 ttgtactcaa gcaaattaat taatagaaac aaaccaagca ccacacctgc actgtacagt
     7321 gtgcccttca gaagctgatt cgtatagaat tttctgataa aatgatccag tttctgaatc
     7381 agtaaggtat aattgtcatt aaattctgcc atattaaaat cactttaagc caaagtatta
     7441 aaccacaaaa tctacctaat gttgcacttt aatattcctt gaaccgggca atttaaactt
     7501 gttttactta tcaatcaagc aggtatgcgt taagaaaagc ataaaatcat cctgcagcaa
     7561 attattcaag cttaaaccgt ttgtaggcat tttgaagttc agggtctttt tcgttgctaa
     7621 tcatttcgcc catctttgac ttgatgtagt ccatgtcaat gttaaagcca gtaggttcga
     7681 gttcttgttt ggccattgca tccagcacat acacctggaa aaagcccaaa acagcagcaa
     7741 atctcttgat tttggattcg gcattcatac cctgggcata caatttatca aatccggtac
     7801 gtgcttttgc agcatctgat tttgcaagca aggtagcata gttgcctgta taagagataa
     7861 gggtatagtc atcgagtttt gacattccgt tttcgataat actaagtttt tcggtttttc
     7921 ctgcatctgc atataacttt gccaaagatg tcaacatggc ttcacttgca tttttctcaa
     7981 atttttctgc atattccatc gctttttctg gtaaaaactg atgtacaagc aataacattc
     8041 ttgctttttg tgcttctgtc cctttagtca aaagttcatt tgccattgat ctgaaaagtt
     8101 caggattgtt ttcgccaata aatgaggttg ctgcactcct tacttcctct tttgggtcgt
     8161 tgttggcaag tgcttttatt ttatccatga tgccggcttg gtttgggtcg catcttacaa
     8221 cagctgccgc tcttattgcc tccagtggat cattaatggc tatgcccatc actgtcctgt
     8281 attcgtcctg gccaccgtca atagcctgtg cgattttgtc gatagcatca taccgtgcca
     8341 gtgccggaga agtcggatta tacaggtggg tgagtatggt atcttcacca taatctgtac
     8401 gctcgcatag aattattttt tctgggtcta tttctacgag taatggtttg gccggtacct
     8461 tgaacctgta ttgttggttt ctttcattta cccagcgttc gtattttact ttgttgcctg
     8521 actggtctgt aatatatact gcaagcggga agcgaaatac tttcggtacg tcggcttggc
     8581 tctgtgtctg tattacatct atcaccacct cttcgcttgt ttcatcataa gacttataat
     8641 attccagttt tgggtgaccc gaggcgttat accattggtc gaaaaaccaa ttaaggtctt
     8701 gcccggaagc atcttcaaat gccattcgga gattatgtac ttctactgct gtaaaggcat
     8761 ttttggcaag ataattttta agtgctttaa agaatacctt atctccgagc atatttcgca
     8821 acatgtgtag tacgagtccg cctttgttgt agctgtgtgc atcaaacatg tcggtctttt
     8881 cattgtagtg gtaatgtatc aatggatgga cattgtattg ggcactcgcc atat
//
LOCUS       DAOQQY010000094         8920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_2998_length_8920_cov_4.466667, whole genome shotgun sequence.
ACCESSION   DAOQQY010000094 DAOQQY010000000
VERSION     DAOQQY010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2998_length_8920_cov_4.466667"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="PLE23_06025"
     CDS             <1..1527
                     /locus_tag="PLE23_06025"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HMX82858.1"
                     /translation="KTDVDAVVVSSSKIRNELLKCNLPAANNLLGHPYLIAGKVIGGN
                     KIGQSIGFPTANIEIDDKNKLILPNGIYSVYVTYDNEKYQGMLYIGNRPVVKDDDKLT
                     IEVNIFDFSSSIYGAILAIDVIEFLRDDIAFDTLDALRTQLIKDEEHARQSLGEYEAQ
                     LAQRKNSSKSVAVVILNYNGIEHLKTFLPSVVECTPNHQIYVADNASTDKSISWVQEN
                     YPDVAIISLPQNYGFAEGYNRALQQLNEEYFVLLNSDVETTPFWLDKMMELTADPEVA
                     AVQPKILSYKDKTSFEYAGASGGFVDALFYPFCRGRMFLTIEKDEGQYNNKREIFWAS
                     GACMLVNADLFRKLGGFDKDYFAHFEEIDFCWRLHKAGYKVMVNPEAVVYHLGGGTLN
                     YDSSHKTYLNFRNSLYTISKNEFLLNLIWVLPFRVLTDLLFVIYRLLTGKINHSLAIL
                     KAYVFFIFNIPALFYKRMQYNEKIDSIKLDDAPNTDGVFHGSILWTYFAMNRKTFRKL
                     "
     gene            1530..2252
                     /locus_tag="PLE23_06030"
     CDS             1530..2252
                     /locus_tag="PLE23_06030"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMX82859.1"
                     /translation="MNIKPEVIFSDQWIIIVNKPAGFLSIPDRFDAAKPNILAYLRRD
                     FPEAMVLHRLDKETSGIMIFARDPDTHRKLSTDFEDHSIRKTYLALAEGYIREDVGRI
                     DKPIGESRSAAGTMIISPNGKPSQTLYQVVKRYKKYTLAEADILTGRMHQIRVHFKSI
                     GHPLAIDAVYGNSDKLFIYDIKQKGMSYSKFDEEKPLMSRTTLHAFALELIHPTTKEK
                     MRFEAPLHKDFKALLNQLDKWG"
     gene            2363..3724
                     /gene="purB"
                     /locus_tag="PLE23_06035"
     CDS             2363..3724
                     /gene="purB"
                     /locus_tag="PLE23_06035"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMX82860.1"
                     /translation="MLQALSPVDGRYFNSTKVLRKYFSEHALIYYRVFVEVKYLSILL
                     NENDVKLNSSKELNHFLDDIIENFCEADAESIKATEKITNHDVKAVEYFIREKLKSAG
                     FHDILPFVHFGLTSQDINNTAFPLMIKNAMTDVVIPEILNLIRQIYAIADTARDLPML
                     ARTHGQAASPTTVGKEMFVFVNRLEHQLKQLIAVPNTAKFGGATGNFNAHLVAYPNID
                     WEAFGNQFVEQVLGLRRNPVTTQIESYDDLAALCQACSRINTVLIDFCRDVWTYVSLD
                     YFKQKVKSGEVGSSAMPHKVNPIDFENAEGNLGVANALLNHFAEKLPISRLQRDLTDS
                     TVLRNIGVPFAHMLIAFKSLDKGINKLTLNTVKIEKDLTDNWAVVAEGIQTILRREGI
                     EDAYEQLKDLTRGAEGINKDSVNRFIDQLPVSDTVKQQLKAITPSNYTGNASAQVRKF
                     TEW"
     gene            complement(3692..3880)
                     /locus_tag="PLE23_06040"
     CDS             complement(3692..3880)
                     /locus_tag="PLE23_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82861.1"
                     /translation="MYQVFNMGHRMELYVSSKETAESIIAISKKYAIDAQIVGRVEDA
                     ERPEVVIRSPFGEFSYLS"
     gene            complement(4884..5399)
                     /locus_tag="PLE23_06045"
     CDS             complement(4884..5399)
                     /locus_tag="PLE23_06045"
                     /inference="COORDINATES: protein motif:HMM:NF040422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6438 domain-containing protein"
                     /protein_id="HMX82862.1"
                     /translation="MRLYYLLLGSLILLSACCKKNTADTINGSGQMELLFETSESPYV
                     VAFIKRTACFGKCPAYEARIYSDGKVTYRGQSNVEMLGNWEAKIELKRLNDIVKKAFD
                     IKYFLLEDQYPDPSVRITDVPTTFTGITCKGQKKNISNKFEGPEKLKVFEDEMDKVIA
                     SLKFTSVDPVK"
     gene            complement(5402..7306)
                     /gene="yidC"
                     /locus_tag="PLE23_06050"
     CDS             complement(5402..7306)
                     /gene="yidC"
                     /locus_tag="PLE23_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HMX82863.1"
                     /translation="MNKEKLNNAIGFGLIFLILFAWMQFNKPSREKLEAEKRTRDSIA
                     QLQTPKPDTPANDSITNAPGLSTSGTVPDSVATQRLGAVLGSFAGAGTGTNQTITLEN
                     DVMKVDFASKGGRITKVELKKYFKVAHDSITKKDVKLPLYLLEDPKNVFNYKLPIADA
                     ANKTVNTTDLYFQSQINGKKVSFKAYAGDGKYIEQLYELKDGSYGLQYQINTIGLGSV
                     WDQSQNTFNLYWENWLDKIEKNSSYERNYSTVYFKPTDENPDYCSCTKSATEEVKLPV
                     KWVSHSNQFFNSSLLAKNSFKSLTAETEVLPEKSPDLKRIKSTITFENPNPNSQSYAM
                     DFYIGPNEFKTLKAYGNSFEDVISFGSSIFGTINRWVFHPLFMLLMKILGSAGLSILA
                     LTLLVKLVLLPLTYKMMYSQAKMASLKPFIAKIKEKHPDDTQTQQVETMSLYREYGVN
                     PLGGCLPLVLQMPIWFALYRFFPAAIEFRQQSFLWATDLSSYDSIYNFSKAIPMYGDH
                     VSLFSLLWMVTTLAYSYYTMKDQEMSGQLGGQNAQMMKVMQYAMPVVFVLFFNNFAAG
                     LTCYLVFSNLLNISQNLAIKRFFIDHDKIREMLEVNKSKPKKRSAFQERLDMAMKEQQ
                     KRQAEKKQNK"
     gene            complement(7458..8519)
                     /locus_tag="PLE23_06055"
     CDS             complement(7458..8519)
                     /locus_tag="PLE23_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMX82864.1"
                     /translation="MKNLTLLLLVLAFVSCKKDDDVVQYDPTPYTLEYGNFPAPKLPA
                     DNPLTVAGVELGRMLFYEQMLSKDGTQSCATCHKAEDNFNDIRRFSIGVDGGEGRRNA
                     MSVSNLAWHNTGFFWDGRAATLREQALKPIQDPVEMNETLVNVVTKLKSSKMYQDQFT
                     KAFKDPEINSDKVALALEQFMFTMVSHDSKYDKWLKGTAQLTDSEERGRVLFFTEKDP
                     FNNIKGAECFHCHNGFDFSNHSITNNGLDADADFKDLGRFEVTKNASDKAKFKTPSLR
                     NIELTPPYMHDGRFVTLEEVIEHYNSEIKQSSTLDPLLYTIQAGLELTPQDKADLIAF
                     LKTLTDQNFTTNPKFQTPF"
     gene            complement(8584..>8920)
                     /locus_tag="PLE23_06060"
     CDS             complement(8584..>8920)
                     /locus_tag="PLE23_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HMX82865.1"
                     /translation="LFTNFHFENNGLYVNYDDEGRKRLTGKDKDEALFKVPTLRNISV
                     TAPYMHDGSFQNLRQVIEHYNKGGAGHHNQSKLVKPLHLTEYEKECLIEFLNTLTDTE
                     FLSDKKYRL"
BASE COUNT         2679 a         1793 c         1679 g         2769 t
ORIGIN      
        1 aaaaccgatg tggacgcagt ggtggtcagc tcatcaaaaa ttagaaatga attactcaaa
       61 tgcaatctac ccgctgccaa taatctgctg gggcaccctt atctgatagc aggcaaggtt
      121 ataggtggta ataaaatagg acagtcaata ggttttccta ccgccaatat tgaaatagac
      181 gacaaaaata aactcatact acccaatggt atttactccg tttatgtcac atacgacaat
      241 gagaaatacc agggtatgtt gtatatcggc aatagacctg tcgtaaaaga tgatgataaa
      301 cttaccattg aagtaaatat ttttgacttc agtagttcaa tatatggtgc cattctggca
      361 attgatgtta ttgagtttct ccgtgacgat atagcttttg acacacttga cgcattgagg
      421 acacagctta tcaaggatga agaacatgca agacaaagcc ttggagaata cgaagcacaa
      481 ctggcacaaa ggaaaaattc aagtaaatca gtagctgttg tcatacttaa ctataatggc
      541 attgagcacc ttaaaacatt tttgccatca gtggtagaat gtacaccaaa tcatcagatt
      601 tatgtagcag ataacgcctc tacagataaa tctatcagtt gggtacaaga gaattatcct
      661 gatgtagcta tcatctcatt acctcaaaac tacggatttg cagagggtta taatagggca
      721 cttcaacaat taaatgaaga atactttgtt ttgctgaatt cggatgttga gacaacaccc
      781 ttttggctag ataagatgat ggaactcaca gcagacccag aagtagcagc agtacaacct
      841 aaaatattgt cctacaaaga caaaacatca tttgaatatg ctggtgcttc gggtggattt
      901 gtagatgctc ttttttatcc gttttgtcgc ggtcgaatgt ttcttacgat agaaaaagat
      961 gaaggccaat acaataacaa acgtgaaatc ttctgggcaa gtggtgcctg tatgcttgta
     1021 aatgctgact tattccgaaa attgggagga ttcgataagg attactttgc tcatttcgaa
     1081 gaaatcgact tttgctggag acttcacaag gcaggataca aagtgatggt taaccctgaa
     1141 gccgttgtat atcatcttgg tggaggtacc cttaactacg attcgagtca caagacttat
     1201 cttaatttca gaaacagcct ttataccatt tcgaaaaatg aatttttact aaatctcatt
     1261 tgggtattac cattcagagt actgaccgat cttctctttg ttatataccg tctgctaaca
     1321 ggaaaaatca accattcgtt ggcaattctg aaagcttatg ttttctttat attcaatatt
     1381 ccggcattgt tttataaacg gatgcaatac aatgagaaaa tagattctat aaagcttgac
     1441 gatgctccga atactgacgg tgtattccac ggttccattc tttggactta ctttgccatg
     1501 aaccgaaaaa cgttcagaaa gctgtaacta tgaacatcaa accagaagtt atcttttctg
     1561 accaatggat aataatcgtt aataaaccgg ccggattttt atctatccct gacagatttg
     1621 atgccgctaa gccaaacatt cttgcttacc tcaggcgaga ctttccggag gcaatggtat
     1681 tacaccgcct tgataaggaa acttctggca tcatgatatt cgctcgtgac cctgacacac
     1741 atagaaaact ctcaactgat tttgaagatc atagtattcg caagacatat ttggcacttg
     1801 ctgaaggtta tatacgtgaa gatgttgggc ggatcgacaa accaatcggt gaaagtcgtt
     1861 ctgctgcagg cactatgatt atttcgccta acggcaaacc atctcaaact ctctatcagg
     1921 tggtaaaacg ttataaaaaa tatacacttg ctgaagcaga catccttacc ggacgaatgc
     1981 accagataag ggttcacttc aaatcgattg ggcatccact ggctattgat gcagtctatg
     2041 gcaactccga caaactcttc atatatgata ttaagcaaaa agggatgtct tattcaaaat
     2101 tcgatgagga gaaaccactc atgtctagaa caacattaca tgcctttgca ctagaattaa
     2161 tacatccgac aacaaaggaa aaaatgagat ttgaggctcc tctacataaa gattttaaag
     2221 ccctactcaa tcaacttgat aaatggggtt gattatcttg caattagtta tcattttcac
     2281 catttcatca atagattagc tcaataaaag ctttataatc gtactttaat caattgtcac
     2341 taactttgca aaaaatttta atatgctaca agcactttct ccggttgatg gcagatattt
     2401 caattcgact aaggtattac gtaaatattt ttcagaacac gcactcatct attatcgtgt
     2461 ttttgtcgag gttaaatatc tcagcatatt gctgaatgaa aatgatgtaa aactaaacag
     2521 cagtaaggaa ctcaatcatt ttctggacga tataattgaa aatttctgcg aagcagatgc
     2581 tgaatctata aaggcaacgg agaaaattac caatcacgac gtaaaagcag ttgaatattt
     2641 tattagggaa aaattgaaat ctgccggatt tcatgacata cttccttttg tgcatttcgg
     2701 acttacttct caggacataa acaacactgc ttttccattg atgataaaaa atgcaatgac
     2761 agatgtcgtt attcctgaaa tcctcaattt gatcagacaa atatatgcaa tagccgatac
     2821 tgcccgagac ctacccatgc tggcacgtac acacggacag gctgcaagcc cgaccacggt
     2881 aggcaaagaa atgtttgtct ttgtcaacag acttgaacat caactgaagc aactgattgc
     2941 agtaccgaat actgcaaaat tcggaggtgc aaccggcaat ttcaatgccc acctggttgc
     3001 ttatccgaat attgattggg aagcattcgg aaatcagttt gtagaacaag tattggggct
     3061 cagaagaaac ccggtaacaa cccaaataga atcttatgac gatcttgcag ctctttgtca
     3121 ggcttgttca aggataaaca cagtactgat agacttttgt cgtgatgtat ggacttacgt
     3181 gtcattggat tatttcaaac aaaaagtaaa atccggtgaa gtaggatcat ctgctatgcc
     3241 acataaggtt aaccctattg atttcgaaaa tgcagaagga aatttgggcg ttgccaatgc
     3301 cctgctaaac catttcgcgg agaaactccc gatatcaaga ctacaacgtg accttaccga
     3361 tagtacagta ctacgtaaca ttggtgttcc gtttgcacac atgctcattg ctttcaagtc
     3421 tttggataag ggcattaaca agcttacgct caataccgtt aaaatagaaa aagaccttac
     3481 agataactgg gctgtggtag cagaaggaat ccaaactatc cttaggcgtg aaggtataga
     3541 agatgcctat gagcaactca aagacctgac ccgaggtgcc gaaggcatta acaaggattc
     3601 tgtaaacaga tttatagatc aactgccggt gtcggatact gtcaaacaac agctaaaggc
     3661 aataacacct tcaaattata ccggtaatgc ctcagctcag gtaagaaaat tcaccgaatg
     3721 gtgacctgat gactacttcc ggtctttcag catcttctac cctacctacg atctgtgcat
     3781 caatggcata tttcttagaa atagcaatga tgctttcagc agtttccttg gaagacacgt
     3841 atagttccat tctatgtccc atattgaata cctggtacat ttctttccat gaggtaccac
     3901 tttcttcctg tataagctta aataatggtg gcaatgcaaa caaattgtct ttaacaactc
     3961 tgaccttgtc agaaacttga tgactttcgt ctgtccacca cccgaacaat gaataatacc
     4021 acttattgag gctttgtatg ctgataaaac ttctttcaat acaggcatat aggtacgtgt
     4081 aggcgacaag actaacttgc ctaccgtcat tttctctcca gctatttcga ttacgtcatc
     4141 aacatttttt gaacctgtaa acactacatc atccggcaaa ttcgggtcaa agatttcagg
     4201 atacttctgg gcatactttt tatgaaaaac atcatgacgt gcagaagtaa gaccgttaga
     4261 acccattcca ccgttatagg atgattcata taaagctttg ccatctgatg ctaaacctac
     4321 aatatagtct ccaggcctta tattattgac taccaaatca ttgcgcttca ttcttgcaaa
     4381 tgttgtaaag cctacatcta ccgtgcgaac aatatcgccg acgtctgccg tttcgccacc
     4441 tgcgagtgtc acatgtatgc caaaactttc aagtttactt aaaaaatctg atgtcccatt
     4501 gatcaattta cttaagactt cagcaggtat gagtctttta ttcctgccaa ttgtgctgga
     4561 tagtatgatg ttatccaagc agccgacaca agccatgtca tcgagattca taacgatggc
     4621 atcaattacg acatccgacc atacactgag gtcatcagtt tctttccaat acaaataagc
     4681 caggctcgtt ttagtaccag ctgtatcggc atgcataata ttgcagtatt caggatcacc
     4741 accggtaata tcaggcaaaa ctttacaaaa agcctttgga aacaatcctt tgtcaagatt
     4801 ttcaattgct ttgtgtactt catcttttga tgcagaaaca cctcttttgt catataaatt
     4861 ttggccgtcc gacatgtatt gatttattta acagggtcaa cggaagtaaa tttcaaactt
     4921 gcaatcactt tatccatttc atcttcgaac accttgagtt tctcagggcc ttcaaattta
     4981 tttgaaatat tttttttctg ccctttgcaa gtgataccgg taaaggtagt tggcacatct
     5041 gttatccgca cacttgggtc aggatattgg tcttcaagca aaaagtattt gatatcaaaa
     5101 gccttcttaa caatatcatt tagtcttttc aattcaattt tcgcctccca attgcccaac
     5161 atttcaacat ttgactgtcc tctgtaagtt accttgccgt ctgagtatat tcgggcttca
     5221 taagccgggc acttaccgaa gcatgctgtt ctttttataa atgctaccac gtaaggtgac
     5281 tcagatgttt caaaaagcaa ctccatttgt cctgaaccat ttatagtatc cgcagtgttc
     5341 tttttgcaac aagcagacaa caaaatcaaa ctcccaagta acaggtaata aagtctcatg
     5401 attacttatt ttgtttcttt tctgcctgtc ttttttgttg ttccttcatt gccatatcaa
     5461 gtctttcttg gaatgcagaa cgttttttgg gctttgattt gtttacttcc aacatctcac
     5521 gtatcttatc atggtcaata aagaatcgtt taatagcaag attctgtgaa atattaagaa
     5581 ggttactgaa taccaaataa caagtaagac ctgctgcaaa attattgaag aataacacga
     5641 acaccaccgg cattgcgtat tgcatcacct tcatcatttg tgcattctgc cctccaagct
     5701 ggccggacat ttcctggtct ttcatggtgt agtaagaata agccaaagta gtgaccatcc
     5761 ataacagcga aaacaagctc acatggtcgc catacatagg aatggctttc gaaaaattat
     5821 aaatcgaatc gtacgaagaa agatccgttg cccaaaggaa actctgctga cgaaactcaa
     5881 tagcagccgg gaagaatctg tataatgcaa accagatagg catttgcaat actaaaggca
     5941 ggcaaccacc cagaggattc acaccatact cgcggtacaa gctcatggtt tctacttgct
     6001 gtgtctgtgt atcatcagga tgtttttcct ttatttttgc gatgaaaggc tttaaagatg
     6061 ccatcttagc ctgcgaatac atcattttat aggtaagtgg caataagacc agttttacaa
     6121 gcaatgtcaa ggcaagaata gaaagtccgg cactgccaag tattttcatc agcagcataa
     6181 acaatggatg aaaaacccat cggtttatag taccaaatat agatgaacca aaggaaatta
     6241 catcttcaaa actattgcca tacgccttca gtgtcttaaa ctcgttgggc ccaatgtaaa
     6301 aatccatggc atacgactgg ctgttagggt tcggattttc gaacgtaatg gtggacttta
     6361 ttcgcttcag gtcaggtgac ttttccggca atacttctgt ttcggcagtg agcgatttga
     6421 aggaattctt tgcaagaaga gacgaattaa agaactgatt ggaatgactt acccatttga
     6481 caggaagctt tacttcttca gtggcagatt tggtacagct acaataatcc ggattttcgt
     6541 ctgtaggttt aaaatatacg gttgaataat ttctctcgta ggaagaattt ttctcgattt
     6601 tgtcgagcca gttttcccag tagagattaa aagtgttttg agattggtcc cagacagaac
     6661 ccaatccgat tgtattaatt tgatattgca atccatacga tccgtccttg agttcataga
     6721 gttgttcgat gtattttccg tcaccggcat aggctttgaa cgatactttc ttaccattta
     6781 tttgtgactg aaaataaaga tctgtggtat tgacagtttt gttggcagca tctgctatag
     6841 gcagtttgta attgaaaacg ttcttaggat cttcaagcaa gtataatggc agttttacat
     6901 cttttttagt aatactatca tgcgctactt tgaaatattt cttaagctct actttggtaa
     6961 ttctaccgcc ttttgatgca aaatctacct tcattacatc attttctaat gtaatggttt
     7021 gatttgtacc tgttcctgca ccggcaaaac ttcctagaac agcacccagt cgttgcgtgg
     7081 ctacagaatc aggtacagtg ccggaagtac ttaaacccgg agcgttcgta atgctatcat
     7141 ttgcaggagt atcaggtttg ggtgtctgta attgtgctat actgtctctg gttcttttct
     7201 cagcttcaag tttttctctg gatggtttgt tgaattgcat ccatgcaaaa agtatcagaa
     7261 atatgagccc gaaaccaatg gcattattaa gtttctcttt attcatttgc gaattttcct
     7321 ttgtctggtt ttattgaatt gcaaaggtat gaaattaatg atgattcgca ttcattcaca
     7381 aatattttaa ataatacctt ttccgttcga ataaactcac aaccgaatat attaaaaatt
     7441 gaatcagctt ttgtcgttta aaaaggcgtc tggaacttcg gatttgtggt aaaattctga
     7501 tctgtaagtg ttttcaggaa agctatcagg tcagccttat cttgtggagt aagttcaagc
     7561 ccggcttgta tggtatataa cagtggatca agtgtagaag attgcttgat ttccgaattg
     7621 taatgttcga ttacttcttc aagtgttaca aaacgaccat cgtgcatgta tggtggtgtt
     7681 agttctatat ttcgtagtga cggagttttg aattttgcct tatctgaagc attttttgtc
     7741 acttcgaacc gccccaaatc cttaaaatca gcatctgcat caagcccatt gttggtaata
     7801 gaatgattgc taaagtcgaa tccgttgtgg caatggaagc actctgcgcc tttgatattg
     7861 ttaaaaggat ctttctcggt aaagaacaac acacggcccc tttcttccga atcggttagc
     7921 tgtgccgtgc cttttaacca tttgtcatat ttcgaatcgt gtgacaccat cgtaaacata
     7981 aattgttcga gagcgagagc aactttatca gaattgattt caggatcttt aaatgctttg
     8041 gtaaactgat cttgatacat tttgctgctt ttgagttttg tcactacatt tacaagtgtt
     8101 tcattcattt ctaccgggtc ttgaatgggt ttcaatgctt gttcacgtag ggttgcagct
     8161 ctgccgtccc aaaaaaatcc ggtattgtgc caggcaagat tacttactga catggcattt
     8221 cgtctgccct caccaccatc tacgccaata ctgaagcgac gtatatcatt aaagttatct
     8281 tcggctttat gacaagtggc acaagactgc gtaccatctt tgctcagcat ttgctcataa
     8341 aacagcattc tgccaagctc aacgccggct acagtcaaag gattgtctgc aggtagctta
     8401 ggtgcaggga agttaccgta ttcgagtgtg tacggtgttg ggtcgtactg caccacatca
     8461 tcatctttct tgcaagatac aaatgctaat accaataata aaagtgtgag atttttcata
     8521 agtgttaatt tataatgttg ctataaaagt aattctttct tagtatttgc agaaatttat
     8581 tggctacaat ctgtattttt tatcacttaa aaattctgtg tctgtcagtg tattaagaaa
     8641 ctcgattaag cattcttttt cgtattccgt caggtgcaat ggttttacca acttgctttg
     8701 gttatggtgc cctgccccac ccttgttgta gtgttctatt acctgcctga ggttttggaa
     8761 agaaccgtcg tgcatgtaag gtgctgttac tgaaatgttt ctgagtgttg gtactttgaa
     8821 gagtgcttca tccttatcct ttccggtaag gcgttttctg ccttcatcat cataattgac
     8881 atacagtcca ttattttcaa aatgaaaatt agtaaaaagc
//
LOCUS       DAOQQY010000095         8848 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3040_length_8848_cov_11.417946, whole genome shotgun sequence.
ACCESSION   DAOQQY010000095 DAOQQY010000000
VERSION     DAOQQY010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8848
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3040_length_8848_cov_11.417946"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..970
                     /gene="nadC"
                     /locus_tag="PLE23_06065"
     CDS             110..970
                     /gene="nadC"
                     /locus_tag="PLE23_06065"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMX82866.1"
                     /translation="MLPYYVTPDKIDWFVNQALIEDVGSGDHTTLACIPDTQSDKAIL
                     KVKDEGIIAGIELAKVIIQKLDPNCSFTTFIKDGDSVSYGDIAFELSANSHALLKAER
                     LILNTMQRMSGIATMSNRFAFEVEDLPVTILDTRKTTPLLRFLEKWAVKLGGCENYRF
                     GLYDWIMIKDNHVEASGGIKNAINNVHRYLKDNKLDLNITVEVRNLVELYDALEVGGF
                     KRIMLDNFEIPLLQEAVEIIGNRYETEASGGITINTVRNVAKTGVRYISSGALTHSFQ
                     SLDLSLKIVR"
     gene            complement(1498..3039)
                     /locus_tag="PLE23_06070"
     CDS             complement(1498..3039)
                     /locus_tag="PLE23_06070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82867.1"
                     /translation="MKKATSLIWILIWISGGVSAQKEYENNWILGYGVSNDNWKYHIG
                     GMRVHFEGGEPVVDTMNVGPSDTFVTTMSDEAGELIFYSNGCSIFNSNYQVMDSGNHL
                     NPGEVYDSYCDSEEDGTSYYPNFNLIISFPRPSHPGQYEVFHVAAEDGTYLSKTLYQT
                     SIDMNVPGGLGKITAKNKELFSKTIVADALAACRHGNGRDWWILMPMVGEKTIYRFLL
                     TPDTTLGPYAQQVGYDKKPYVLTPGQCCFSPDGKHYVHADPGNGVNLYDFDACTGLLS
                     NAQRYYYAPDTAFCSGAAFSPNSRFLYVSTGVKLYQYDMWSPDIGASRIVIDDVNANP
                     APSPASIFVGRLAINDKIYFSSTGTIGYLHVINKPDEAGKACDFVQQGYELPRLNDWI
                     VPNIPYFRLFDQPGSPCDTLGIDAPISTKYVTAKSIISVFPNPVSSTLSIDVEQAGYG
                     SVREQTVDITDLLGRRLYTVDIDYDGHAEIDTQALPPGMYIAVLRTDGHQVQAVKFVV
                     VRSER"
     gene            complement(3085..5604)
                     /locus_tag="PLE23_06075"
     CDS             complement(3085..5604)
                     /locus_tag="PLE23_06075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82868.1"
                     /translation="MLHVNIEIISGGELRLFNSRLFAFTTTWNGIEVKDNGFLVLARN
                     RIEDAENAVSIDIGAKGVNLNGNVFNRNRVAIRNGVASFTPGIMKFGYFADNIFTCSS
                     LLYQNSPSDPDWSIAGIQLDNCVATITSNVNKPNHFFRLENGIKSHGASYVINNCYFY
                     DNFRIDEGNNKIGYAINANGGTVNVSYGFSQNLGLLNCYFGTTESKNPYIWTQKASCR
                     LSKACFEGGDQFILNTDNNSGQVIDIWQNNMDLKNYTALGGVETERPVGSGNFTRVTY
                     TENKVRLSAQAGGKSDFSAGLFLHSNYSSTDRTAITRNEVNVYNNGWGNVGICLVGTG
                     DNNFIKYNTVKSFSEANGNRQGLALHSFKGKNNYIAENTVDGQGDRHNFTSAFFADES
                     PGISWCHNHADYSGIGFHFLGNCDYTQFRSNVNRRHDIGLFIEGKFGTGLIGEQVRRS
                     NLWSYTPDVDYTILAAKCEGNAAYSRFITEGEYIEIKPTKIDPQNDWFKSEPGILDNC
                     NNEQLPVIDTTSVYDRSLINNDTTGWTAYELWHSTKALMKRMIEDPAGTSADVGKYYF
                     YNTHLNTNVGKLADIEVQGRNKIETEDTYSIIIGQSVNNINRLMDEIVSLQSQLVTDT
                     VHYESDSTFEVSRDAKLDSIVYYRNAEEDARASLLASRTPFYNTVKNILANMVPQNQF
                     EQNAIQYNKILIDKWMGNGLTASQLDSLEAIASQCIQKGGEYVKLSAQLLGSKKLKYY
                     MATENITCTEPRAAEHINGQKVSEMARLYPNPTEGILSIEVSLPADYIIYDALERVVE
                     AGSLEEGSRHQLDMSGFQSGIYYVKFRFVDGSSLVSKIIKI"
     gene            complement(6375..7292)
                     /locus_tag="PLE23_06080"
     CDS             complement(6375..7292)
                     /locus_tag="PLE23_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82869.1"
                     /translation="MKNLMIIIVLLTCYQQFVNAQKVPEFETTFYFEDAKGNRDSVII
                     GYDPSVPEYIGGINGLTPQFGEQPLQEPFDTILDVRVDPEGNAGGDPFGWFKRMIGFC
                     EKFPPGVCYGGTVGGFVISNKYPPLKISYSQKAFGTPNLGGSFLTHDKFYDLIGASAL
                     PYNHYFCLSDTGEHIIDFSDSSYVNGYWGLFKQNVPMKGGDVANLAQINFYLLVGDCL
                     YHTATSEVKSALLKAYPVPATDRIVIELPEELLFSYKELAVSDVVGRTSMLKLIEEAD
                     TTIGLDISSLSPGFYFGRIDRFVLKFVKQ"
     gene            complement(7297..>8848)
                     /locus_tag="PLE23_06085"
     CDS             complement(7297..>8848)
                     /locus_tag="PLE23_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M43 family zinc metalloprotease"
                     /protein_id="HMX82870.1"
                     /translation="HQYISTSAKSVWQGCMILLFTFFINYPQLCYSQNYPPITCSEYL
                     PSDITIATPENFTAICTDPNSVKYLRVNYHFILKDDGTGNFNEYGDGQGNGNQYNGFV
                     RAKEIIENANSELSALTENWLPSGETKTVLPTRFRYILTGVYFHRDSKMFNKDHSTNY
                     FLAGHKKYDINPESSINVYDTKIIGASGFATGIGVQQGDYTCFVDNYDAYLAYPTWDA
                     HPAQLLNHEIGHILDLRHSFSAPSTDKCDDTPPNPNCWAFTGTPPCDTWNNISNNIMD
                     YNQYYPHAYTPCQVGRIHDYLNGPKGNYIIHSCNACKPANALAWIEREKCVLPRKFYQ
                     VKLNCQGSYNVDKYLLEICEIADNDNLDCTGGYYNSGWINGAPGVLDLSSVYTFTPDK
                     HYKIRFSGDNTDGPSLSSSVNIIYTKDCGETGTSDPHIIINPIVAPNPGSGLRTISFS
                     LSKQADVSIYLVNGSTSQIQSQILDSYSLQPVLNLFGFNDSGLANGQHYVTITADNEL
                     ISIPFIIN"
BASE COUNT         2542 a         2005 c         1613 g         2688 t
ORIGIN      
        1 tattgtactc agggtaggtg tattgtttta accatcacac cttacaccca acattctaaa
       61 actacacaga gcatgaatca aaaaattcag gcctgcaaat caatgcttta tgctaccata
      121 ttatgtcaca cctgataaaa tcgactggtt cgtaaatcag gcacttatag aagacgtagg
      181 ctcaggcgac catacaaccc tcgcttgcat tccggatact cagtcggaca aagccatact
      241 taaagtcaag gacgaaggta tcattgccgg tatagagctg gcaaaagtca tcatccaaaa
      301 actcgacccc aactgcagct tcactacatt catcaaagac ggtgacagcg tcagctacgg
      361 cgacatcgcc ttcgaactct cagccaactc tcatgctctg ctcaaggccg agcgccttat
      421 actcaacacc atgcaacgca tgagtggtat cgccaccatg agcaaccggt tcgctttcga
      481 agtcgaagac ctacccgtca ccatcctcga cacccgaaaa accacgcccc tacttaggtt
      541 cctcgaaaag tgggccgtca aactcggcgg atgcgaaaac taccgcttcg gtctctatga
      601 ctggattatg attaaagaca accacgtcga agcaagcggc ggtatcaaaa acgcaatcaa
      661 caacgtccat cggtacctca aagacaataa gctcgacctt aatatcaccg tcgaagtacg
      721 caacctggta gaactatacg acgcactcga ggtaggcggg ttcaaaagga tcatgctcga
      781 caatttcgag attccactcc tccaggaagc cgtcgaaatc attggcaacc gttacgagac
      841 tgaagcatca ggcggcatca ctatcaacac cgtgcgcaac gtcgctaaga caggcgtccg
      901 gtacataagc tccggagccc tgacacactc tttccaaagc ctcgatctca gcctgaaaat
      961 cgtccgttaa caccaacgac agtttttgtt gatttgacat tcagatgtat catacctcaa
     1021 ggatagcctt gggcgtgccc ctgtcgggtc aggctatccg ccttacttcg tgccggcttc
     1081 tatccttcac gcggattggg taagttgtaa gttgttagtt gtaagttgtt agttgtaagt
     1141 gaaatgtaag atgtatgctg ttgtgaaaca ttaatattca gctttttttg aaccattcag
     1201 aagttaagcg tatgaagcag atactgcgtc ggagcaagca gaaagttgta taacaaaatc
     1261 tccgttttgt ttgcaccttc cctaaaggaa gcaaaacgaa gattttgatg ggaaattgaa
     1321 ctgtttttct ttccgcctcc cttcagggca ggggcaagcg gaaagagagc cacttttttt
     1381 gccagaatca ggatttttag gattccggga ttttcaggat tttatttcgg acgatgatgt
     1441 cgtaggccgg gcaagcagaa agttgtataa aaaccaacac cattcactac ttaccactta
     1501 ccgctcactt ctcaccacca caaacttcac tgcctgcacc tgatgcccgt ctgtgcggag
     1561 cactgctatg tacatacccg gtggtaatgc ctgcgtatct atttcggcat ggccgtcgta
     1621 gtctatatct accgtatata gccttctgcc caacaggtcg gtgatatcta ctgtctgctc
     1681 acgcacgcta ccatagcctg cctgctcaac gtctatgctg agcgtggagg agacagggtt
     1741 gggaaaaaca cttattattg actttgccgt tacatacttt gtagatattg gcgcatctat
     1801 cccaagtgta tcgcaagggc tacccggttg gtcaaacaat cgaaaatatg gtatattggg
     1861 cactatccaa tcgttcagcc ggggcaattc gtacccttgc tgtacaaaat cacatgcctt
     1921 cccggcctcg tcgggcttgt taatcacatg caagtaaccg atagttcccg ttgaagaaaa
     1981 atatatcttg tcgttaatgg ccaatcgccc tacaaatata ctagccggag agggcgcagg
     2041 gtttgcattt acatcgtcta ttactatacg cgatgctccg atgtccggcg accacatatc
     2101 atattggtac agcttcaccc ctgtgctaac atacaaaaat cgactgttcg gactaaatgc
     2161 agccccagag caaaatgctg tatccggtgc ataataatat cgctgagcat tgctcaataa
     2221 accggtgcag gcatcaaaat catacaagtt gacaccattg cctgggtcag catgtacata
     2281 atgcttccca tctggactaa aacagcactg gccgggtgtc aagacatatg gtttcttgtc
     2341 ataccctact tgctgagcgt atggcccaag cgtagtatcg ggtgtcaaca aaaatcggta
     2401 aatcgtcttc tcacctacca ttggcatcag tatccaccag tctcgcccat taccatgccg
     2461 gcaggcagca agcgcatctg ccactatcgt ctttgaaaac aattccttgt ttttggccgt
     2521 tatcttacct agtccgccag gtacattcat gtctatactc gtctgataaa gtgttttcga
     2581 taaataagtg ccatcttccg ctgcaacatg gaacacttca tattgcccgg gatgggaggg
     2641 ccgaggaaaa ctgatgatta aattaaaatt cggataatac gatgtgccgt cttcctcaga
     2701 atcgcaatag ctgtcataca cttcaccggg gtttagatga ttgccactat ccattacctg
     2761 gtagttgcta ttaaatatcg aacagccgtt gctgtaaaat atcagctccc cggcttcgtc
     2821 actcatggtg gtcacaaatg tatctgatgg accgacattc atagtatcaa ctaccggctc
     2881 acctccttca aagtgcaccc gcattcctcc tatgtggtat ttccagttat catttgacac
     2941 tccataacca agtatccagt tgttttcgta ttccttttgt gcactaacac cgcccgatat
     3001 ccatatcaat atccatatca aacttgttgc ttttttcatc tgacaaactt tggcttaatc
     3061 ataaaattta tgaaatggta cttgttatat ttttataatc ttactgacta atgagcttcc
     3121 atctacaaaa cgaaacttta cataatatat cccgctttgg aagcccgaca tatccagctg
     3181 atgtctggaa ccctcctcga gacttcctgc ctctactact cgttcgagtg cgtcgtatat
     3241 gatataatca gctggcaaag atacctcaat gctcaggatg ccctcggttg ggttcggata
     3301 taatcttgcc atttctgaaa ctttctggcc gttgatatgt tccgcagccc ttggttctgt
     3361 gcaagtaata ttttctgttg ccatataata ctttagtttt ttactgccaa gcaactgtgc
     3421 tgataatttc acatactccc ctcctttttg aatacactgt gaagctattg cctccaaact
     3481 atcgagctgc gatgctgtca atccattacc catccacttg tctatcaata ttttattata
     3541 ttggatggca ttttgctcaa actggttttg tggcaccata ttcgccaaaa tattttttac
     3601 tgtattgtaa aacggagtac gactggccaa aaggctagcc cttgcatcct cttctgcatt
     3661 tctgtaatac acgatagaat ccagcttcgc atcacggctc acttcaaatg tactatccga
     3721 ctcataatgc actgtatctg tgaccaattg gctctgtagt gacacaatct catccatcaa
     3781 tcggttgata ttatttactg attgaccaat aataattgaa taagtatcct ctgtttctat
     3841 cttattcctc ccttgcacct ctatatccgc caatttaccg acattggtat ttaaatgggt
     3901 attgtaaaaa taatatttgc ctacatctgc agatgtaccg gcagggtctt ctatcatcct
     3961 tttcatcaaa gctttggtac tgtgccacag ctcgtaagct gtccaacctg tagtatcgtt
     4021 gtttattaag cttctgtcat atacgcttgt agtgtcgatt actggcagct gctcattgtt
     4081 gcaattatca agaatgcccg gttcagactt aaaccaatca ttttgtgggt caatttttgt
     4141 gggttttatc tctatatatt ctccttcggt gatgaacctt gagtaggcag cgtttccttc
     4201 gcactttgct gcaagtatag tataatcaac atcaggtgtg tatgaccaaa gattggaccg
     4261 gcgtacttgc tcaccgatta atccggttcc aaatttgcct tctatgaaca aacctatgtc
     4321 atgtcttcta ttaacattgc ttctaaactg ggtataatcg cagtttccga gaaaatggaa
     4381 accaatacct gagtaatcgg catggttatg acaccaactt ataccaggac tctcatccgc
     4441 aaaaaaggca ctcgtaaaat tgtgtctatc tccttgccca tcaacagtat tttctgctat
     4501 ataattattt ttccctttga agctatggag ggccaagcct tgtctgttgc cattcgcttc
     4561 tgaaaatgac ttaacagtat tatatttgat aaaattgttg tctcccgtac ctacaagaca
     4621 gataccaaca ttaccccacc cattgttgta aacatttact tcattgcgtg tgattgcggt
     4681 acggtcagtg gaggaataat tgctatgaag aaaaagtcct gctgaaaaat cgcttttacc
     4741 gcctgcctgt gcagacaaac gaactttatt ctcagtatag gtaactcttg taaaattacc
     4801 cgacccgacg ggtctttctg tttccacacc gcccagtgcg gtgtaattct tcaggtccat
     4861 attgttctgc catatatcaa tgacctgccc actattatta tctgtattga gtataaattg
     4921 atcaccaccc tcaaaacatg ctttcgacaa acggcatgat gccttttgtg tccatatata
     4981 cgggttttta gattcggtgg tgccaaaata gcaatttaac aaaccaagat tctggctaaa
     5041 tccataacta acattaactg tacctccatt ggcattaatt gcatatccta ttttattgtt
     5101 cccttcatca attcggaaat tatcataaaa gtaacaatta ttaataacat aacttgctcc
     5161 gtgtgacttt ataccattct ccaacctgaa gaaatgatta ggtttgttta catttgatgt
     5221 aatggtagca acacaattat ctaattgtat tcctgctatt gaccaatcag ggtcacttgg
     5281 cgaattttga tataaaagag atgaacatgt aaaaatatta tcggcaaaat agccaaactt
     5341 catgatacct ggtgtaaaag aggcaactcc gttcctgatg gctactctat ttcgattaaa
     5401 tacgttacca ttcaaattaa ctccttttgc tcctatgtct atacttactg cattttcagc
     5461 atcttctatt cgatttcttg ccaatactaa aaagccatta tcttttactt cgatgccatt
     5521 ccatgtagtg gtgaatgcaa acaatcgtga attaaataat cttaattcgc cacctgaaat
     5581 aatttctata ttaacatgta acatctctaa ttcacaattt ataaacatta atggtacatc
     5641 cacaatcaag tccgaattaa tccttatctt ttgattaaaa tatgtgttct ttggtaattg
     5701 tgatgcaaaa ataacccatg taccttgggg ggcaaataaa tcaacatcag ttggcttatt
     5761 atgaaattcg tcacgaagtg ttaggacggg attaaaactt ttcttgctta taggaccaca
     5821 tgaaacaggc ccatcttgag aataaccgtt aagtaaaaaa taaaacacaa aacacaaaat
     5881 caaaccaatc cttaaaccaa caaaataact gctatttatt tgttgggctg ttgggctgtt
     5941 gggctgttgg gctgttgggc tgttgggcta atgtttttca tactataaaa gtttatcatt
     6001 tattggatga cctaaaggta caatacttaa cattgatatc caaacatttt tagaaatttt
     6061 tttcaattat tttacctgtt tgttttttat aaaatgccaa tacagtatat tattctgatt
     6121 acacaaatta ttacaaacat caacagatga aaatttggct gtggtgtagg ctacccgata
     6181 gattgttttg ggacttggcc ggacattcgt ctggagataa gcggctggtg gcacattttg
     6241 ctttcacgat cactgatttc gcggattaca acaatgggtg tagattgttt gctaaaaatc
     6301 ttaaatgctt aatgccgcac ttcactttct cagtttacaa tggatgacta ttttgttggg
     6361 aggaataaac aacattactg cttcacaaac ttaagtacaa acctgtcaat ccttccaaaa
     6421 taaaatcccg gactcagcga gctgatatcc agaccaatag ttgtatcggc ttcttctatc
     6481 agctttaaca tgctcgtcct gcccacaaca tcagacaccg ccagttcttt atagctaaat
     6541 aataactctt ccggcagctc tattactatt ctgtctgtcg ccggcacagg gtaagctttc
     6601 aaaagtgctg acttaacctc tgacgtcgct gtatgataca aacaatctcc aactaataaa
     6661 tagaaattga tttgtgcaag gtttgcaaca tcacctcctt tcataggtac attttgcttg
     6721 aacaaacccc aatatccatt gacataagat gaatcactga aatctatgat atgttcacct
     6781 gtatcactca ggcagaaata gtgattgtat ggcaatgcac tagcacctat caaatcataa
     6841 aatttgtcat gcgttaaaaa tgacccacca agattaggtg tgccaaatgc tttctggcta
     6901 taacttatct taaggggtgg gtatttattg ctaataacga aacctccgac tgtgcctcca
     6961 taacacacac cgggaggaaa tttttcacag aagccaatca tacgcttaaa ccacccgaaa
     7021 ggatcaccac ccgcattacc ttccggatca actcgtacat ctaatatcgt gtcaaatggt
     7081 tcttgtagag gctgctcacc aaactgcggt gtaagcccgt ttatgccgcc aatatattct
     7141 ggcacacttg gatcataacc tataataacc gagtctctat taccttttgc atcttcaaag
     7201 taaaaagttg tttcaaattc aggcactttt tgcgcattca caaactgctg atagcaagtg
     7261 agtaatacta ttatgatcat tagatttttc ataatattag tttataataa atggtatgga
     7321 aatcaattca ttatctgctg ttattgttac atagtgctga ccgttcgcaa gacctgaatc
     7381 attaaagcca aataaattca atacaggctg caatgaataa ctgtcaagta tctgtgactg
     7441 gatttgactt gtgcttccat tgacaagata tatactgaca tcggcctgct tactcagact
     7501 aaagcttatg gtccttaaac cactgcccgg attcggtgca acaatcggat ttataataat
     7561 atgtggatct gatgttcctg tttcaccaca atcttttgtg tatattatat tcacacttga
     7621 gctcaatgag gggccgtcag tattatctcc agaaaatcta atcttataat gcttatcagg
     7681 agtgaatgta tatacacttg ataaatcaag tactcctggg gcgccattaa tccatccgga
     7741 attataataa ccgccagtgc aatctagatt atcgttatct gctatttcac atatctcaag
     7801 taagtattta tcaacattat aacttccctg acaattaagt ttgacctggt aaaacttgcg
     7861 cgggagaaca cacttttcac gctctatcca agcaagagca ttggcaggct tgcaagcatt
     7921 acagctatgt atgatataat tgcccttagg gccattcaaa taatcatgaa tccttccaac
     7981 ctgacatgga gtataggcat gtgggtaata ttgattgtaa tccataatat tgttgctgat
     8041 attattccat gtatcacagg gtggtgtacc agtaaatgcc caacagttgg gattgggagg
     8101 tgtatcatca catttatcgg tacttggcgc cgaaaatgaa tgccgcaaat ccaaaatatg
     8161 gccgatttca tgattcagta attgagcagg gtgagcatcc catgttgggt atgccaagta
     8221 tgcatcataa ttgtccacaa aacatgtata atcaccctgt tgaaccccta taccagtagc
     8281 gaagccactg gcaccaatta ttttcgtatc atatacattg atacttgatt ccggatttat
     8341 atcgtatttc ttgtgtcctg ctaaaaaata gttagtacta tggtctttgt taaacatctt
     8401 tgagtctctg tgaaaatata cccctgtaag aatatatctg aatcgggtag gtaacacagt
     8461 ttttgtctct ccactcggca gccaattttc agttaaagca ctcagttctg aattagcatt
     8521 ctcaattatt tctttcgctc tcacaaagcc attatactga ttaccatttc cttgaccatc
     8581 tccatattcg ttaaagttac cggtaccgtc atcctttagt ataaaatgat aattaactct
     8641 taagtactta acagaatttg gatctgtaca tattgccgtg aaattttctg gagtcgctat
     8701 agtaatgtcg gaaggtaagt actcagaaca agtaatgggc gggtaattct gactgtaaca
     8761 caactgaggg taattaataa aaaatgtgaa taacagaatc atgcaacctt gccaaactga
     8821 cttagctgat gtactgatgt actgatgt
//
LOCUS       DAOQQY010000096         8616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3153_length_8616_cov_5.624343, whole genome shotgun sequence.
ACCESSION   DAOQQY010000096 DAOQQY010000000
VERSION     DAOQQY010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8616
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3153_length_8616_cov_5.624343"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLE23_06090"
     CDS             complement(<1..731)
                     /locus_tag="PLE23_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMX82871.1"
                     /translation="MSNLFVKKSISLLMSHAEDSEKNLKKTLTATSLIALGIGAIIGA
                     GLFVRTAAAAAQNAGPSVTIGFIIAAIGCALAGLCYAELSSSIPIAGSAYTYSYATMG
                     ELVAWMIGWDLVLEYAVGAATVGIAWAEYLNNLLTNVLHLPPIPYEWCHSPFEMSVSG
                     VHGIINLPALFIVSLLSLLLIRGTHESAIVNNLIVVAKVTIVILIIATGWHFINPANH
                     AVYIPEPSVYVDHQGISHSYGGIMG"
     gene            complement(1076..1330)
                     /gene="rpsT"
                     /locus_tag="PLE23_06095"
     CDS             complement(1076..1330)
                     /gene="rpsT"
                     /locus_tag="PLE23_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HMX82872.1"
                     /translation="MAHHKSSLKRIRQDAKKRLENRYYKKTTRSAIKTLRAITDKAEA
                     EKFLPRVVSMIDKLAKKNNIHKNNAANLKRGLTLHINAMA"
     gene            1512..4244
                     /locus_tag="PLE23_06100"
     CDS             1512..4244
                     /locus_tag="PLE23_06100"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HMX82873.1"
                     /translation="MKDYSYIFNATPSFIDTMYAQYLSDPNSVEPGWRTFFEGFEFSG
                     KSISAPTKLSGSNNADFDKEFSVVSIINGYRRRGHLLSDTNPIAQRKNRFPRLDLKDF
                     GLSDADLDRQFIFGKEIGLPNATLKEILQKLNKIYCGSIGFEYKHIENYEKRQWLRER
                     IENRPLDNYAYSAEKKKRILSKLNGATVFEKFLHTKYIGQKRFSLEGGETTIPALDAI
                     INKAADADVEEFVIGMAHRGRLNVLANVLGKTYENVFNEFEGIAIPDLSYGSGDVKYH
                     LGFSSQVETASGKKVHLKLAPNPSHLEAVDPVVLGFSRAKADILYESQFNRILPILIH
                     GDAALAGQGVCYETTQMSLLKGYLTGGTLHFVINNQVGFTTDFDDARSSTYSTGLAQV
                     VQAPVFHVNGDDPEAVVFVSELAVEYRQKFGCDVFIDMVCYRRHGHNEGDDPQFTQPI
                     MYKRIAEHPTPREIYVSQLTQRGDIDLQIAIEMEDTFWAELQARLDMVKQKHLPYSYQ
                     EPEQAWRSLRKEVLPKYFEKSPATGVSSTILKSIADHLMRIPEGFTPLTKIHRLLKAK
                     EEGIAKNEYDWAMGELMAYGSILLENRDVRLSGEDVKRGTFSHRHAVFYDAETNKEYN
                     RLAHLADQQGEFRIYNSLLSEFAVLGFEYGYALASPNNLVIWEAQFGDFVNGAQTIID
                     QFITTGMTKWHRMNGLVMLLPHGYEGQGPEHSSARLERWLQNASELNMAITNITTPAN
                     FFHALRRQLAWDFRIPLVVMSPKSLLRHPECVSRLEDFSENTSFLEVIDDKSIANTAR
                     VTKVLLCSGKIYYDLIKYRRDNKLENTAIVRIEQLYPFPASQLDEILKKYKTAKIVWV
                     QEEPVNMGAATFVQQMLGKSLSEMVARAHVASPASGFKRVHDTEQAELLKDAFA"
     gene            4244..4927
                     /gene="aat"
                     /locus_tag="PLE23_06105"
     CDS             4244..4927
                     /gene="aat"
                     /locus_tag="PLE23_06105"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HMX82874.1"
                     /translation="MPVYWLNDNDVRFPPLEYAMPDGLLAIGGDLSVKRLLGAYKCGI
                     FPWYNATEPIMWWSPDPRLVLFPDKLHVAKSMRTYFNNNTFQLSYNHAFEEVMTACGE
                     ISRKNQDGTWIHQDLVDAYVRLHKAGFAHSVEVWKNSRLVGGLYGIAIGKVFFGESMF
                     AHLPNASKFGFISLVKKLAQLNFKLIDCQQETPHLKSLGAELLDRNVFVQILQENDKK
                     FSHEKLKLD"
     gene            4957..6207
                     /locus_tag="PLE23_06110"
     CDS             4957..6207
                     /locus_tag="PLE23_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMX82875.1"
                     /translation="MQAVDQYFDRIIIGGGAAGFFAAVQCASMAPHLSIAILEKGNDV
                     LSKVRISGGGRCNVTHACLDPDVLTTFYPRGNKELRGPFRTFNCEDTIAWFESKGVKL
                     KTEPDGRMFPTTDDSSTIIKCLYEEARQKQVKILTATRVDSFSKKDSHFLLNTNKGQF
                     SCEKLMVASGSSAAQWSTLSALGHKIVPSVPSLFTFNIQDARIDDLQGISLECVEISC
                     PEINHKTLSAQNGPLLITHWGMSGPAVLRLSAWGARIFHEKEYRFDISINWLYPMHRS
                     QILETILDYKHSNNSKQKILTHYPFRQIPIRLWKNLLTRALKHSAEKNWADLNKREMD
                     IIVEVLCDSKFKVNGKSTFKEEFVSAGGVDLGEINLKKFESKIIPGLYIAGEALNIDA
                     VTGGFNFQAAWTGGHIAGCSMAED"
     gene            6232..6999
                     /locus_tag="PLE23_06115"
     CDS             6232..6999
                     /locus_tag="PLE23_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMX82876.1"
                     /translation="MSQQLYSKITGQGPPVIILHGLLGTSDNWHSIAANLAEHYTVCL
                     LDLRNHGRSFHSHDFNYELMAQDVMRFMSDNWIQSASVIGHSMGGKVAMYLALHEPDL
                     VDRLLVADIGVKDYPPGHLHIFDALSSVNLNQLTERRQAQDFLEVRLQNKDVVLFLMK
                     NLTRDKEGHYHWKMNLPAIIANYVNIMKGFHESLIFDKPTLFVKGSRSEYIKDDDRAG
                     IRSFFPNATFSSIEGAGHWLHADNPEAFLQICLTFLD"
     gene            7871..8296
                     /locus_tag="PLE23_06120"
     CDS             7871..8296
                     /locus_tag="PLE23_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82877.1"
                     /translation="MKQTNFGATARKFVLGLVMLFGCMLLSGSMSAQSVASKTASFTK
                     SGTLKEPAVAKFDLKAKVEELKGNANTNPDMAYILKANYWSATLASLENGATTEQADK
                     DGVAKAAQIYNHYNAVDPTALEGVNVEGAIETARQLVKK"
     gene            8371..>8616
                     /locus_tag="PLE23_06125"
     CDS             8371..>8616
                     /locus_tag="PLE23_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82878.1"
                     /translation="MKKMNFTLRVWSLLCMAFLGWGVANAQCPAPAGPYPTAVTQLPA
                     TLVVTAGTFANEVGFQLNGAGILTPINVASNGGGTTTN"
BASE COUNT         2625 a         1814 c         1719 g         2458 t
ORIGIN      
        1 atgcccatta tgccgccata actatgcgat atgccctgat ggtcaacata tacgctcggc
       61 tcaggaatat aaacagcatg attggcagga ttaatgaaat gccagcctgt agcaattatg
      121 agtataacga ttgtcacttt cgcaacaacg atcaggttgt taactatggc tgattcgtgc
      181 gtccctctga taagcaacag actcagcaag cttacaataa acagtgccgg caaattgatg
      241 ataccatgta caccacttac cgacatttca aagggcgaat gacaccattc ataaggtatg
      301 ggtggtagat gtaagacatt ggttaacagg ttgttgagat actcggccca ggcaatacca
      361 actgtggctg cacctactgc atattctagc acaagatccc agcctatcat ccaagctact
      421 aattcgccca tcgtagcata agaatatgta taagcactac ctgcaattgg aatagatgaa
      481 gataactcgg cataacacaa gcctgcaagc gcacaaccga tagcagcaat aatgaatccg
      541 atggtgacag atgggccggc attctgtgcg gctgctgcag ctgtccttac aaaaagaccc
      601 gctccaataa tggctcctat accaagagca atgagtgatg tggctgttaa tgttttcttt
      661 agattttttt cgctgtcttc agcatgtgac atcagtaaac tgatggattt ttttacaaaa
      721 agatttgaca tcgggtgaat tagttagttg gcgtaaaaat aatcaggttt tggtattttt
      781 aaaactattt aatacactca agttgctttt ttttcctggc ttgaatgttc gaattggtca
      841 aagttgcctt tcaaggctga aaatgctaaa gaatccatat atttttactt attcaactaa
      901 gccttttttt cttttaaaaa atctgcattt tttcgaaatt ttttattctg ccataaagat
      961 ggaaagtagt tttcaccaga acttttgttt cagttacgct attttatcaa ccgaattcaa
     1021 actaaaaaag gccttccact attgcggaaa gccttttttg gcattattaa gaatgttatg
     1081 ccattgcgtt aatgtgcagc gttaatcctc tcttgaggtt ggcagcatta tttttgtgta
     1141 tattattctt cttggctagc ttatcaatca tacttactac gcgaggtaag aatttttctg
     1201 cctcagcctt gtctgttatt gctcgaagtg tcttgatagc cgaacgggtt gtttttttgt
     1261 aatatctatt ctcaagacgc ttttttgcat cttgtcttat tctttttaag gatgatttgt
     1321 gatgtgccat atcaattttt tatttcgggt ggcaaagata gatgaatctg ctttaaaaat
     1381 caaaaataag taaaattatt tttaaatttt ccatttttaa taaacatttt aacagcattt
     1441 tacttgtgaa gatataaaaa tcttagcaac ttcgtattat atttattcaa ataaaattat
     1501 tcttccatat aatgaaagat tattcctata tttttaatgc gacgccttca tttattgata
     1561 cgatgtacgc tcagtatctc tctgatccta actcggtaga acccggctgg aggacattct
     1621 ttgaaggatt tgaattctcc ggaaaatcaa tttctgcacc tacaaagctt tccggtagca
     1681 ataatgctga ttttgacaaa gaattttcag tggtatctat tatcaacggt taccgacggc
     1741 gaggacactt actttctgat accaacccga tagcccaaag gaaaaatcgt tttccacgac
     1801 ttgacctgaa agatttcgga ttgtcggatg ctgaccttga tcgacaattt atctttggca
     1861 aagagatcgg acttccaaat gctacactta aagaaatatt acaaaagtta aataaaattt
     1921 actgtggcag cattggtttt gaatataagc acatcgaaaa ctacgaaaaa cgacagtggc
     1981 tccgcgaacg aattgaaaac agacctctgg ataattatgc ctattcagcc gaaaagaaaa
     2041 aaagaatact gtctaaactg aacggcgcaa cagtttttga aaaattctta catacaaaat
     2101 atatcgggca gaaacgtttt tcactcgaag gtggtgagac aaccataccg gcacttgacg
     2161 ccatcataaa taaagcagca gatgccgatg ttgaagaatt cgtgatagga atggcacacc
     2221 gcggcagact caatgtgctg gctaacgttc tgggaaaaac ctacgaaaat gtattcaacg
     2281 agtttgaagg tattgccatc ccggaccttt cttatggctc aggcgatgtg aaataccacc
     2341 ttggtttctc atctcaggta gaaactgctt caggcaaaaa agtacacctg aaacttgctc
     2401 ccaatccttc tcaccttgaa gcagttgatc cggttgttct tggtttttca agagctaaag
     2461 ccgatattct ctacgagtct cagtttaacc gtattttacc catactcatc cacggtgacg
     2521 cggcgctggc cggtcaaggt gtttgttacg aaaccactca gatgagcttg ctcaaaggtt
     2581 atctgaccgg cggaacattg cacttcgtca tcaataatca ggtcggtttt accactgact
     2641 ttgatgatgc acgatcttct acatatagta caggacttgc tcaagtagtt caagctccgg
     2701 ttttccatgt caatggcgat gaccctgagg cagtagtatt cgtctccgaa ctggcagtgg
     2761 agtatagaca aaaatttgga tgcgatgtgt ttatagatat ggtctgctat cgaagacacg
     2821 gccacaacga aggtgacgac ccacagttta cccaacctat catgtacaaa cgtatcgcag
     2881 aacaccctac accacgcgag atttacgttt cacaactaac ccaacgcggt gatattgacc
     2941 tacagatagc aatagaaatg gaagatactt tctgggctga actacaagca agattggata
     3001 tggtcaagca aaaacatctc ccgtacagct accaggaacc cgaacaggcc tggcgctcac
     3061 tcagaaaaga ggttttgcca aaatattttg aaaaatctcc tgccacgggc gtcagtagta
     3121 caattctaaa atccatagct gaccacctga tgcgtattcc cgaagggttt actccgctta
     3181 ctaagataca ccggctactc aaagcaaaag aagaaggaat agcaaaaaat gagtatgact
     3241 gggcaatggg tgaattgatg gcttacggat caatactact cgaaaaccga gatgtaagat
     3301 taagtggaga agatgtaaaa cgaggcactt tctcacacag gcacgccgta ttctatgatg
     3361 ctgaaaccaa taaagaatat aacagacttg cacatttggc tgatcagcaa ggagagttta
     3421 gaatttacaa ttcgctctta tcagaatttg cagttctggg ctttgaatat gggtacgctt
     3481 tggcgtctcc aaataacctc gtcatctggg aagcacagtt cggtgacttc gtcaatggcg
     3541 ctcagaccat tattgatcag tttatcacta ccggaatgac caaatggcac cgtatgaacg
     3601 gactggtcat gctattgccg catggttatg aaggacaggg acccgaacac agtagcgcac
     3661 gcctcgaacg ttggctacag aatgcttccg aactgaatat ggctataacc aacatcacca
     3721 cacctgccaa tttcttccac gctctcagac gtcagttggc atgggatttc agaattccgc
     3781 tcgtggtaat gtctccaaaa tctttgctcc gtcatcctga gtgtgtatca agactcgaag
     3841 atttttccga aaatacttca ttccttgaag tgatagacga caaaagcatt gctaatactg
     3901 cacgtgtgac caaagtattg ctctgctcag gtaagattta ttacgactta atcaagtaca
     3961 gacgagataa taaactggaa aatacagcca tcgtcagaat cgaacaattg tacccattcc
     4021 cggcctcaca acttgatgaa attctgaaaa aatacaaaac agctaagata gtctgggtac
     4081 aggaagaacc tgtaaatatg ggggctgcta cattcgttca gcaaatgctc ggtaagtcgt
     4141 tgtccgaaat ggtggcaaga gcacatgtgg cttctccggc ttcaggattt aagcgtgtgc
     4201 atgataccga acaggccgaa ttgctaaaag atgcctttgc ataatgccgg tttactggct
     4261 caatgacaat gatgtaagat tcccaccgct ggagtatgcc atgccagatg gtctgcttgc
     4321 cataggtggc gatctgagtg tcaagagatt gttgggagcc tataaatgtg gcatttttcc
     4381 atggtataat gccactgaac ccatcatgtg gtggtcaccc gaccctcgcc tggtgttgtt
     4441 tcctgacaag ctccacgttg ccaaaagtat gcgcacgtat ttcaataata atacgtttca
     4501 gctaagctac aatcatgctt ttgaagaagt gatgacggct tgtggcgaaa tttcaaggaa
     4561 aaatcaggat ggcacctgga ttcatcaaga tctagtcgat gcgtatgtcc gacttcacaa
     4621 ggcaggtttt gcacattctg tagaagtttg gaaaaatagc cggctggtag gcggtctcta
     4681 tggtattgcc atcggaaaag tttttttcgg cgaatcaatg tttgcacatc tgccaaatgc
     4741 aagtaaattt ggcttcattt cacttgtcaa aaaactcgca caattgaatt ttaaacttat
     4801 agattgtcag caggaaacac cgcatctcaa atctcttggt gctgaattgc tagacagaaa
     4861 tgtctttgtt caaattcttc aagagaatga caaaaaattt tcacatgaaa aattaaagct
     4921 tgactaagca cacacatata ttatatatat aaagacatgc aagcagtaga tcagtatttt
     4981 gacagaataa tcatcggtgg tggtgctgcc ggtttttttg ctgctgtaca atgcgcatcg
     5041 atggctccac atttatccat agccatactc gaaaaaggaa acgacgtgct cagcaaggtt
     5101 cgtatctcag gcggtggccg atgcaatgtt acccacgcat gccttgaccc tgatgtactt
     5161 actacttttt acccaagagg aaacaaagag cttcgaggac cttttcgtac cttcaattgc
     5221 gaagatacca ttgcttggtt tgaatctaag ggcgttaaac tcaaaactga acctgacgga
     5281 agaatgttcc ccaccacgga tgactcatct accattatta aatgtctgta tgaagaagcc
     5341 agacaaaaac aagtcaaaat actgacagcc accagagtcg attcgttttc aaaaaaagat
     5401 tcacattttt tgctaaatac aaataaaggt caattctcgt gtgaaaaact tatggtagca
     5461 tctggcagtt ctgcagcaca atggtcaaca ctttctgcgc tcgggcataa gatcgtgcct
     5521 tccgtacctt cactatttac tttcaatatc caggatgccc gtatcgatga ccttcaagga
     5581 atatctttgg aatgtgtcga aatcagttgt ccggagatta accataaaac cctctcggca
     5641 cagaatggtc ctctgctcat tacgcactgg ggtatgtcgg gcccggctgt actccggctt
     5701 tcggcttggg gtgcacgcat atttcacgaa aaagaatacc gttttgatat aagcatcaac
     5761 tggctttacc caatgcaccg ctctcaaata ctggagacaa tccttgatta taaacattcg
     5821 aataattcaa aacaaaaaat cctgacccat tatccattcc ggcaaatacc cattcgcctt
     5881 tggaaaaatc tcctgactcg tgccttgaaa cattcagcag agaaaaactg ggcagatctt
     5941 aacaaacggg aaatggatat catcgtggaa gtattgtgtg acagtaaatt taaggtaaat
     6001 ggtaaaagta catttaaaga agagtttgta tcagcaggtg gggtcgattt aggtgaaata
     6061 aatctcaaga aatttgaaag caagattata cccgggcttt acatagccgg tgaagcgctc
     6121 aatattgatg cagtcacagg tggcttcaat tttcaggcag cctggacagg cgggcacatt
     6181 gcaggatgct caatggcaga agattgataa gattatacta tttttgcaaa tatgtctcaa
     6241 cagctttatt caaaaattac aggccagggc cctccggtca ttattctgca cggtctctta
     6301 ggcacatctg ataactggca cagtattgca gccaacttgg ccgaacatta taccgtgtgc
     6361 ctgctcgatt tgcgcaatca tggccgttca tttcactcac acgattttaa ctatgaactc
     6421 atggcacaag atgtcatgcg ttttatgtcc gacaattgga ttcagtcggc ctcggttatt
     6481 gggcatagta tgggcggtaa ggtagccatg tatcttgcat tgcacgaacc cgacctcgtt
     6541 gatcgcctgc tcgtggccga tattggcgtt aaagattatc ctccgggtca tctacatata
     6601 tttgatgcac tgtcttctgt aaatcttaac caactgacag aacgtaggca agcacaagat
     6661 ttcctggaag tccgtttgca gaataaagat gtggtcttgt ttttaatgaa aaatttgact
     6721 cgtgacaaag aaggacatta tcactggaaa atgaacctgc ctgccattat tgccaattat
     6781 gttaatataa tgaaagggtt tcacgaatca ttaatttttg ataaaccaac actttttgta
     6841 aaaggtagcc gaagcgagta tattaaagat gatgaccgtg ccggaataag aagctttttc
     6901 cctaatgcca catttagttc cattgaaggt gctggacatt ggctccatgc agataacccg
     6961 gaagcatttc ttcagatctg tcttacattt cttgattgaa agttgaaaaa taaattcaat
     7021 aaaatgttta aaccaaacca aacttggcat ttgatttgca ataaacagat attgtaataa
     7081 aaaaaatatc agaaaatttt tggaaggata ttgcagttta ttaaatatca acttaccttt
     7141 gtcctgtaat tatatgaaag caaacattag aaacattttt ttgaaaatac tgacagaact
     7201 tacaccgcat ttgcattacc gcatcctcac tcacgcatac cgcttcagac ttatcaccat
     7261 gcgaaccata aattcctaaa taacactcag gttatacata cgatctttgt tccagtattt
     7321 acgaacttat attattacct aatcattgta ccattgacat actacggcat catgaaaatt
     7381 tatctgaaat aaattttgca tagaacgtat atttatttta cctttgcgtg atatattgaa
     7441 aatatttgaa taagaccgga gttcacaaag agaagtgtcc cttaaaccgt aaaaaaaccc
     7501 tctaaccgaa atacttctcc ccactcactc tcatcatttg atgaactcac tatgtaaggt
     7561 cttatacggg agagaatcca ttaaccgaaa tccttaatgg taatgccata aggtacctaa
     7621 gaaagaaaaa aactgtgccg gtgaagctct atgcccaacc agcatgcaag tcaagtagta
     7681 tctcatgaac tatcacctca cccggaacag ccacccggct tttcctccga gactcacctg
     7741 agaatactcc ttgcgaataa aagacagacc gatccaaaaa ccgacttagg cgcaagcctt
     7801 ttgtcacagg atacgtttgt cgcaatggaa cggatttttt aacttatttt ttaaccaaat
     7861 tattaatctt atgaaacaga cgaatttcgg agctactgct cgcaagtttg tgttaggttt
     7921 ggtaatgctt tttggctgta tgcttctgtc aggctctatg tctgcacagt cagtagcatc
     7981 taaaacagca tcttttacca agtcgggtac actaaaggag cctgcagttg caaaatttga
     8041 cctgaaagct aaggtagaag aacttaaggg taatgccaac accaatccgg atatggctta
     8101 catccttaag gccaactact ggagtgcaac actcgcttct cttgagaacg gtgctaccac
     8161 tgaacaagct gacaaggacg gtgtagctaa agctgcacaa atttacaatc actacaatgc
     8221 agtagatcct actgcacttg aaggtgtgaa tgtagaaggt gcaattgaaa cggcacgcca
     8281 acttgtgaaa aagtaattac ttgatctaaa aggtaaaaac aactttcaca agaactttta
     8341 ttaaaaactt taaccaaaat ttttaatctc atgaaaaaaa tgaattttac actgagagtg
     8401 tggtcattgc tttgcatggc attcctcgga tggggcgttg ccaatgccca gtgtccggct
     8461 cctgcgggcc cgtacccgac agctgtaact caacttcctg caacattggt tgtaacggca
     8521 ggtactttcg ccaatgaggt tggtttccaa ctgaacggtg caggtatcct gactcctatc
     8581 aatgttgctt ctaacggtgg tggtactact actaac
//
LOCUS       DAOQQY010000097         8558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3188_length_8558_cov_7.330824, whole genome shotgun sequence.
ACCESSION   DAOQQY010000097 DAOQQY010000000
VERSION     DAOQQY010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3188_length_8558_cov_7.330824"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1983)
                     /locus_tag="PLE23_06130"
     CDS             complement(<1..1983)
                     /locus_tag="PLE23_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HMX82879.1"
                     /translation="MQSDLDIKAMDDDRIIIQRAYRNLLKCIKGTLSQEDQDNIRLAY
                     EMAVKAHSAQRRKSGEPYILHPIAVAQICVEEIGLGPTAVICALLHDVVEDTDITLKE
                     IEETFGERIAKIVDGLTKLDSAYNVDVPQAENFKKVLSTLVEDVRVVLIKMADRLHNM
                     RTLGSMLKHKQLKIAAETTYIYAPLAHRLGLYNIKTEFQDLCMKITDPENYHEIARKL
                     KETKKSRTNYIEDFIKPLKGELDDLNIPYRITGRPKSIYSIYNKIKTKKVSFEEIYDL
                     FAIRIIVDVPIKKEKSACWQVYSVVTDVYSTIPERLKDWITTPKSNGYESLHTSVIGP
                     GGRFVEVQIRSERMDEIAERGYAAHWKYKGMSNQHDVYESWLDSVRDLMENPEADALE
                     FINDFKTNLFAEEVYIYTPKGDLQILPKGSSALDFAFSIHSDLGYHAVAIKVNNKLVP
                     MGYKLKNGDQVHVTTNKSQKPNESWLKMVITGKARSKIRSALKEEKKKQAEFGKEAVE
                     RKLNNLKVDFESNIDTLIKYYGFKTRLDLYSAIASDEIKLSEFKNFKIENNKIVLITP
                     VTEQGLVSHTVDNLELPKVEAKDTDKMKLLIGGESADHYDFSFATCCSPLPGDKVFAY
                     LTSNAGLKIHRQSCANAPNLFANYGYRIMKAEWINIS"
     gene            2136..2217
                     /locus_tag="PLE23_06135"
     tRNA            2136..2217
                     /locus_tag="PLE23_06135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2170..2172,aa:Leu,seq:tag)
     gene            complement(2283..3416)
                     /locus_tag="PLE23_06140"
     CDS             complement(2283..3416)
                     /locus_tag="PLE23_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX82880.1"
                     /translation="MKGSETLYRLIIFWISFAPFIANTQSIRPPELGPQLVVNNGFED
                     IKRIPTKWYYNGEDFNLIMNKWVSPTLSSPDVYTPSTKVPEGWAEKGFGDARPYKGRN
                     MVGLTLYGCEHGKPHCREYIQSMLKEPLIIGQKYYIDFWVKPLKKSMLCNNIGAFFSV
                     FEIKKELDVQLKCKPQLNARDIISPEKNEWIHIEGIFTASTAAKYIVLGNFFADDKTK
                     TMLPGGKYPLNFAYYYFDEISVYKIPPFTEAPSEEGTFDACCIKKGDTIQLHNIYFDF
                     DKSVLRPESYIELNKLLRVLGDRPSMKVHITGHTDIIGHSNYNIWLSRNRANAVREFL
                     IENAVDEDRLKVVAMSYSQPAATNKTEEGRQLNRRVEIIVIEE"
     gene            complement(3435..4259)
                     /gene="ygiD"
                     /locus_tag="PLE23_06145"
     CDS             complement(3435..4259)
                     /gene="ygiD"
                     /locus_tag="PLE23_06145"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HMX82881.1"
                     /translation="MMSLNSLFNLVTDHKIEKPLPIIFLGHGSPMNAIEKNEFTDGWS
                     HLAEHLENVKAILCVSAHWKTKGTYITGMKLPKTIHDFGGFPQVLYDVQYPAPGDPEL
                     AMEIQQHFKDHILIDHDWGLDHGCWSVLMHIFPKANVPVLQLSLDYRKDPEYHYQLAK
                     KLTELRRKGVLIVGSGNMVHNLRALNWTEGSGGYDWAVEANEKFKELINKQDHVALTQ
                     YSKLGKAVQNAVPTPEHYLPLLYVLGMRDKNETAYFFNDKTMMGSISMTSVAFGLN"
     gene            4413..5939
                     /locus_tag="PLE23_06150"
     CDS             4413..5939
                     /locus_tag="PLE23_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMX82882.1"
                     /translation="MEFLKKLGLKKRNAGTWTGIESSTSTLKYLNSFSPVDGQLIGSV
                     SRTSKEEYENVIKKAEEAFKYWRMIPAPKRGEIVRQYGDELRKNKDHLGRLVSYEMGK
                     SLQEGWGEVQEMIDICDFAVGLSRQLYGLTMHSERPSHRMYEQWHPLGIVGVISAFNF
                     PVAVWSWNAMIALVCGDVVVWKPSEKAPMCGVACMNILHKVLKDNSLPEGICGLINGD
                     YKVGEYMTKDERVALISATGSTRMGRIVGSTVAQRFGKTILELGGNNAIIITPDADAD
                     IYLTAAVFGAVGTAGQRCTTTRRLIIHDSIYDDVKKKLAHAYKQLRIGNPLDPSNHVG
                     PLIDTDAVKNYLNAISQAKEEGGKILVEGGVLSGEGYETGCYVKPCIIEAKNDYAVVQ
                     KETFAPILYIMKYKTIDEAIDMQNGVPQGLSSSIVTNNLREAEQFLSCAGSDCGIANV
                     NIGTSGAEIGGAFGGEKETGGGRESGSDSWKAYMRRQTNTINYSKSLPLAQGIKFDLD
                     "
     gene            5990..6781
                     /locus_tag="PLE23_06155"
     CDS             5990..6781
                     /locus_tag="PLE23_06155"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HMX82883.1"
                     /translation="MFNPDTDWRRVTAAIYKKPTDSKIFGSVELDVTHLEEFLNKNRV
                     NGTKLTFTHFFAFAIAMGIHTKVPQLNTFVRRGRIVSRPSIDVGVSVLNHDQTEMNAV
                     VVRDAINYNLHSFTHQLNELIAQSKSGAGVNTKGKYLLAKVPWPFRNWFFRLVRFVTV
                     GLGLFSSSPAGITNRFGSFILSNIGSVGLDVGYAALLPASNVSAVFTLGTVETLPRYI
                     NGQLTPRRIIKLAVTLDHRLVDGLHGGLLFRYLKQVVRNPDLLEK"
     gene            7550..7969
                     /locus_tag="PLE23_06160"
     CDS             7550..7969
                     /locus_tag="PLE23_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82884.1"
                     /translation="MCQFHQSAIIRRYLTKKPKLKAAQELMEVVDLMKRTDKESFVGA
                     LQLWFDKWEYFLNERTINPINGKSFYTHKRLRSAYRSLKNNLSWLFTWYDHFELNIPN
                     TTNAIDGHFADLKNKLRNYNGLSIQRKRKFIDGFLKA"
     gene            8340..>8558
                     /locus_tag="PLE23_06165"
     CDS             8340..>8558
                     /locus_tag="PLE23_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82885.1"
                     /translation="MKKHDLLFLLFGLVQVVVHAQEATIPPDKPGAISGERCIGTQTE
                     VTGENHFSTEKNWNFLLNSDLQVIRHSDP"
BASE COUNT         2573 a         1619 c         1701 g         2665 t
ORIGIN      
        1 gctaatgttt atccattctg ctttcataat ccgatagccg tagttggcga acagatttgg
       61 agcgttggca caggattgtc ggtgtatctt aagccctgca tttgatgtaa ggtaggcaaa
      121 taccttgtca cctggcaacg gactacaaca ggtagcaaat gaaaaatcgt aatgatctgc
      181 actttctcca ccaataagca gcttcatttt gtctgtgtcc ttggcttcaa cttttggcaa
      241 ttcgagattg tcaactgtat gtgacactaa cccctgttct gtcactggtg ttatgagaac
      301 aattttgttg ttttcaattt taaaattctt gaattcggat agcttaattt catcagacgc
      361 tatggctgag taaaggtcga ggcgtgtctt gaagccataa tatttaataa gagtatcgat
      421 gttggattca aaatcgacct ttagattatt gagttttcgt tctactgctt ctttaccaaa
      481 ttcggcttgt tttttctttt cctccttcaa ggcagagcga attttagatc tggccttacc
      541 ggttattacc attttcagcc agctctcatt gggcttttgc gatttgttgg ttgttacatg
      601 tacctggtca ccgtttttaa gcttgtagcc cataggtacg agcttattgt ttaccttaat
      661 ggctacagca tgataaccca ggtcactgtg tatgctgaag gcaaaatcca gtgccgaact
      721 gcctttgggc aatatctgaa gatcaccctt aggagtataa atatagactt cttccgcaaa
      781 aaggttggtc ttgaagtcgt tgatgaattc aagggcgtct gcttcaggat tttccatcag
      841 gtcgcgtacg ctatcgagcc aactttcata cacatcatgc tggtttgaca ttcctttata
      901 cttccagtgt gctgcatagc cacgttctgc aatttcgtcc atccgttctg accttatctg
      961 tacttctaca aacctacctc ccggaccaat tacactcgtg tggagtgatt catagccgtt
     1021 agactttggg gtagttatcc agtctttgag tcgttcgggg atggtactat atacatctgt
     1081 cactacagaa tatacctgcc agcatgctga tttttctttt ttgatgggta catctactat
     1141 tattcttata gcaaacaagt cgtatatttc ttcaaaagat accttttttg tttttatctt
     1201 attatagatt gaataaatag attttggtct gccagtgatg cggtatggaa tattaagatc
     1261 gtcaagctca ccttttaacg gcttgataaa atcttcaata taattggttc ttgatttttt
     1321 tgtttccttt agttttcggg ctatttcatg gtaattttca gggtcagtaa tcttcataca
     1381 caggtcctga aattctgttt tgatattgta cagaccgagc cggtgtgcca aaggtgcata
     1441 tatatatgta gtctcagcag ctattttaag ttgtttgtgt ttaagcatac tgcctaaggt
     1501 acgcatgttg tgtaggcggt cagccatttt tatcaatact acccgaacat cttcaaccag
     1561 ggtggacaat acttttttaa aattttctgc ctgaggtacg tccacattgt atgcgctatc
     1621 gagttttgta aggccatcaa ctatcttagc tattctttct ccaaatgttt cctcaatttc
     1681 cttaagtgta atatctgtat cttcgaccac gtcatgcaat aatgcacaaa taacagcagt
     1741 aggaccgaga cctatttcct caacacatat ctgcgctacc gcgataggat gaagtatgta
     1801 aggttctccg gattttctgc gttgtgcaga atgcgctttt acagccattt cataagccag
     1861 gcgtatattg tcttggtctt cctggctcag tgtcccttta atgcacttca gtaaattcct
     1921 atatgcccgt tgaatgatta ttctatcatc atccatagct ttaatgtcta agtcagattg
     1981 catagtgttt cgttttactt cgttaattac cttactacaa aaataaagga aaaaaattta
     2041 aaaataaatt ctttgtgaat gtgtagcttt tttaattgga atgtgcgtat atttgcactc
     2101 tctttataac acatgaaagt ttgtgtttgt tccatgcggg tgtggcgaaa ttggtagacg
     2161 cactagactt aggatctagc gctgcgaggc atgggggttc gagtccctcc acccgcacat
     2221 cgaacaaatg ttttactgtt gataacagtg acggattttc ttgaaaaatt ttcccaaagt
     2281 atttattctt caattactat tatttcgacc ctacggttga gttgcctgcc ttcttctgtt
     2341 ttgttggtag ctgccggctg agagtagctc attgctacta cttttaacct atcttcatct
     2401 acagcatttt ctatcaaaaa ctctctcact gcattagcac ggtttcttga cagccagatg
     2461 ttgtagtttg aatgaccaat gatgtctgta tgcccggtga tgtgaacctt catactcgga
     2521 cgatcaccca atacacgcaa taatttattt aattcaatat aagattcggg cctgagtaca
     2581 cttttgtcaa aatcaaaata aatgttgtgt agctgaatgg tgtcaccctt ttttatacaa
     2641 caagcatcaa aagttccttc ctctgagggc gcttcagtaa atggaggaat tttgtaaaca
     2701 gatatttcat caaaataata ataagcaaaa ttcagagggt actttcctcc cggaagcatt
     2761 gtttttgtct tatcatcagc aaaaaaattg ccaagtacaa tatatttggc agcagtactg
     2821 gctgtaaaaa ttccctcgat gtgaatccac tcattttttt ccggacttat gatgtccctt
     2881 gcatttaatt gtggcttaca ttttaactgt acatcgagct ctttcttgat ttcgaaaacc
     2941 gaaaaaaatg ctcctatatt attacaaagc atggattttt tcaatggctt tacccaaaag
     3001 tcaatataat atttctgccc tatgattaat ggttctttta acattgactg tatgtattct
     3061 ctgcagtgtg gtttaccatg ttcgcaccca taaagtgtaa gtccaaccat atttctgcct
     3121 ttataagggc gggcatcccc gaagcccttc tcggcccagc cttcaggtac tttagtagat
     3181 ggtgtatata catcgggtga tgaaagagta ggtgataccc atttgttcat aatgaggttg
     3241 aagtcttcac cattgtagta ccatttggta ggaatcctct ttatgtcttc aaaaccattg
     3301 ttgaccacca actgtggccc gagctccggt ggcctgattg actgagtatt agctataaac
     3361 ggagcaaagc ttatccagaa tattattaag cggtacaatg tttcagatcc tttcaaaatg
     3421 aatcctgttt ttaattaatt taaaccgaaa gctactgatg tcatcgatat cgaccccatc
     3481 attgtcttgt cattgaagaa ataagccgtt tcgttcttgt cgcgcatacc aagtacataa
     3541 agcaaaggta agtaatgttc gggtgttggt acagcatttt gtacagcctt acctagtttt
     3601 gaatattgag tcaaagctac atggtcttgc ttattaatga gttctttaaa tttttcattt
     3661 gcctcaacag cccagtcata accacccgag ccctcagtcc agttcagggc tctgagattg
     3721 tgaaccatat tgccacttcc tactatcagc acaccttttc ttctgagttc ggttagtttt
     3781 tttgccagtt ggtaatggta ttccggatct tttctataat ctaggctgag ttgtagaacg
     3841 ggcacattcg ctttagggaa aatatgcata agtacactcc aacaaccatg atcaagcccc
     3901 caatcatgat caatgagtat gtgatctttg aagtgttgtt ggatttccat agcaagctcc
     3961 gggtcgccgg gtgccggata ttgtacatca tataatacct gaggaaaacc accaaaatca
     4021 tgaatggtct tcggcaattt cattcctgta atataagtac cttttgtttt ccagtgagca
     4081 gatacgcaca atattgcttt tacattttca agatgttctg ccagatgact ccacccatct
     4141 gtaaattcgt ttttttcaat ggcattcata gggctaccat gacctaaaaa gatgataggt
     4201 aatggttttt caattttatg gtctgttacc agattgaata aagagtttaa agacatcatc
     4261 tgcgaagata ttgaaaggag tgaaacagac ttaatgaatg tttttctatc cattattcga
     4321 ttatttaaat ataaaacgaa acccagactt aagtgttgta gttatgttga aatcaaattt
     4381 acttaacttt gcacccttaa ataataaaca gaatggaatt tctaaagaaa ctcggactta
     4441 aaaagagaaa tgcgggcaca tggaccggca ttgaatcatc aaccagcaca cttaaatatc
     4501 tcaactcttt ttcacctgtt gatggccagc tgataggtag tgtatcaaga acttcaaagg
     4561 aagaatacga gaatgtaatc aagaaagctg aagaagcttt taaatactgg aggatgatac
     4621 cggcacccaa aagaggtgaa attgtaagac aatacggtga cgaactccga aaaaacaagg
     4681 accatctagg ccgcttagta tcatacgaaa tgggtaaaag tcttcaggaa ggttggggcg
     4741 aagtacagga aatgatcgat atctgtgatt ttgcagttgg tctgtcaaga cagctatatg
     4801 gtctaacaat gcactctgaa cgaccatcgc acagaatgta cgaacaatgg catccgcttg
     4861 gtatagtagg ggtgatatcg gcattcaact tccctgtggc agtgtggtca tggaatgcca
     4921 tgatagccct tgtttgtgga gatgttgttg tttggaaacc atcagaaaag gcaccgatgt
     4981 gtggtgtggc atgtatgaat attctacaca aagtactaaa agacaatagc ttgccagaag
     5041 ggatttgtgg ccttataaat ggtgattata aagttggtga atacatgacc aaagacgaac
     5101 gtgtagcatt gatttcggct accggcagta cgcgaatggg tcggatagta ggctctacag
     5161 tagcacaaag attcggtaaa accattctcg aacttggagg gaacaatgct atcatcatca
     5221 ctccagacgc agatgccgat atctatctta cggcagcagt atttggagca gttggtactg
     5281 caggacagcg atgtactact actcgccgtc tgattattca tgattctatc tatgatgatg
     5341 ttaagaaaaa attggcacac gcatacaagc aacttcgaat aggcaatccg cttgatcctt
     5401 ctaatcatgt tggcccgctc atcgatacag acgctgtcaa gaattacctc aacgctatca
     5461 gtcaggctaa agaagaaggt ggaaaaatac tcgtagaagg tggtgtgttg tcaggtgaag
     5521 gatatgaaac cggctgctat gtcaagccat gtattataga agcaaaaaat gattacgcag
     5581 ttgtacaaaa ggagaccttt gccccaatat tatacatcat gaagtacaaa acaatcgatg
     5641 aggcaatcga tatgcaaaat ggtgtacccc aaggtttatc ttcatctatc gttaccaata
     5701 atcttcgcga agccgaacaa ttccttagtt gtgcgggttc tgactgtggt atagctaatg
     5761 tcaatatcgg aacaagcgga gctgaaatag gtggcgcatt cggtggagag aaagaaacag
     5821 gtggtggcag agaaagtggt tctgactcct ggaaagctta catgcgcaga caaacaaata
     5881 ctatcaatta tagtaaaagt ctgccgctgg ctcaaggaat aaagttcgat ctcgattagt
     5941 aaattcaaaa gctgttgtat cttcaatcgt caaacaacaa ttgaatacta tgttcaatcc
     6001 tgatactgat tggagaagag taacagctgc aatttataaa aagcctacag attcgaaaat
     6061 attcggttca gtagagcttg atgttacaca tcttgaagaa ttccttaata agaacagggt
     6121 gaatggcaca aagttgacat ttacccattt ttttgctttc gccatagcaa tgggcattca
     6181 taccaaagtg cctcagctga atacttttgt aagacgaggg agaatagtgt caagaccatc
     6241 gattgatgta ggtgtaagtg tactcaacca cgaccagacg gagatgaacg ctgtggtggt
     6301 aagagatgca atcaactaca acttgcatag cttcactcat caactcaatg aactgatagc
     6361 tcaaagcaag tctggagctg gtgtcaatac gaaaggcaaa tacctactag ctaaagtccc
     6421 ttggccgttc agaaactggt tttttagatt ggttaggttt gttacggttg gtctagggct
     6481 ctttagctcc tcaccggcag gcattaccaa cagatttggt tcattcatac tgtccaatat
     6541 aggtagtgta gggcttgatg tcgggtatgc tgccttgttg cctgcatcca atgtgtcagc
     6601 cgtttttaca ctaggtactg tggagacctt gccacgatat atcaatggtc aactcacacc
     6661 aagacgtatt ataaaactgg ctgtaacgct tgatcaccgg ctggttgacg ggctccacgg
     6721 cggcttgctt ttcaggtatc tgaaacaggt agtgagaaat cctgatttac tggaaaaata
     6781 atataatcgt taattgtaga tatttgataa atactttact taggatgatt ttctgaaaaa
     6841 taatcagaaa tacttggaat tagctcacct gatatattta agcttttcat ttcttttaat
     6901 aagaaactaa tacataaatt taatttatcg ttaatcgtag aaataggata aagtaaaatt
     6961 tagtattcaa tatttaaaat tgggcttaat ggacaaaatt aatgctaaaa acgctcaaaa
     7021 gccttaaaat cattagcttt gaagccaatc aaaggttatg aataaaaaag tgcttttact
     7081 gtggtagctc aatcacaaag aaaaatggtt ttgtaaacaa gattcaacgc tacaaatgtt
     7141 atgcttgtgg caagcaattt ttaggaggaa tcaggataaa caaccaacaa ctatggaaag
     7201 aatatcaaga agggaaacaa acgtatgtcc aacttgccca aaaataccat tgttctataa
     7261 agactattca gcggcgaata gacacagtcc aagttaaacc caatagtaaa agcccaagaa
     7321 atgtaattgt tctgatggac acaacgtatt gggggcgagg ttttggagta atgttgttta
     7381 aggatagttt gacaaaggaa aatctattga agtattatat caaaactgaa acaaacgcct
     7441 tgtatattca ggggataaaa gaattaaagt ctatgggttt cagattagtg ctatcgtttg
     7501 cgatggcaga aaatgtctta tccaatcgtt tgaaaatatt ccggttcaaa tgtgtcagtt
     7561 ccatcaatcg gcaattatcc gtagatattt aaccaagaag cccaaattaa aggctgctca
     7621 agaattaatg gaggttgttg atttgatgaa gcggacagat aaagaatctt ttgtgggagc
     7681 attgcaatta tggttcgata aatgggagta tttcctgaat gagcgaacca tcaatccgat
     7741 caatgggaag tccttttata cacacaaaag attgcgtagt gcatacagaa gtttaaaaaa
     7801 taacctgtct tggctgttta cctggtatga tcatttcgag cttaatattc ctaatacgac
     7861 caatgctatt gatggtcatt ttgctgactt gaaaaacaaa cttcgcaatt ataacggctt
     7921 gtctatacaa aggaaaagga aatttatcga tgggttttta aaggcataaa gttctctaaa
     7981 aatatcaaag agctacgtag ggtagctcct tgaaaatgac attttcgtcg aacatctgcc
     8041 ctatccctga caagttgctc cccagcagag cttgtttccg tttagacaga taaagcaaaa
     8101 gtctgtaaaa aattctttgg ttgcaacaaa gcgataaaaa taaacagcat taaaaatgtc
     8161 cattagaaca catcgtctaa aaaatagcat taattttgtc cattactcct aaaattgata
     8221 tttttactaa ccgttttgtg gccatcataa aaatattatc aataataaat tgggttatat
     8281 agaatgtttt ttgtatattt gaagatattc aaccaataat tccaaaatac cagtacaata
     8341 tgaaaaagca tgatttatta tttttgcttt ttggtttggt acaagtagtt gtacacgcac
     8401 aggaagcaac aatacctcca gacaaacccg gcgcgatttc aggagagaga tgtataggta
     8461 cacaaacaga ggtaacaggc gaaaaccatt tttcaaccga aaaaaactgg aatttcctcc
     8521 tcaacagcga tttgcaagta atacggcata gtgatcct
//
LOCUS       DAOQQY010000098         8509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3211_length_8509_cov_4.872960, whole genome shotgun sequence.
ACCESSION   DAOQQY010000098 DAOQQY010000000
VERSION     DAOQQY010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3211_length_8509_cov_4.872960"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLE23_06170"
     CDS             <1..628
                     /locus_tag="PLE23_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMX82886.1"
                     /translation="STSHPKDITDEVLHTMAAYHNICNYIHLPVQSGSSRILELMNRT
                     YDREWYIDRIDAIRNIIPDCAISTDVISGFCTETEEDHQETLSIMEYAQYSMAYMFFY
                     SERPGTLAARKYEDDIPEEVKKRRLNEVIEMQRAISLKHNLNDIGKRYEVLIEGDSKK
                     SGEQFKGRNSQNKMIVFDKIDGIKAGDYTNVEVTEATSATLLGKIIDI"
     gene            657..1964
                     /locus_tag="PLE23_06175"
     CDS             657..1964
                     /locus_tag="PLE23_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMX82887.1"
                     /translation="MQNLLSIKQRFGIIGLSKSLDQALDVAVRVAATDLTVLITGESG
                     VGKEVFSKIIHSLSNRKHNPFIAINTGAIPPGTINSELFGHEKGSFTGATGDRKGYFE
                     TVNGGTIFLDEIGEMPLDTQAFLLRILESGEFIRVGSSAVHKTDVRVIAATNVDLTQK
                     VKEGKFREDLYYRLNTVPIRVPSLKERKEDIPMLFRKFCIDFSEKYRSSVVQLDEEAT
                     NILINYSWPGNIRELKNVAEQVSVLANNKIVTSEDLVSFVPNLLVRNLPAFSPDLGNS
                     SSSGGYYERELLYKLLFEMKKDISDLKGLIFELINANDLNISDVNSLVSLQAESYPRY
                     NADVAYPTDRQGPSFNPQPVKYAPEAGMEEKPNTLVINHPNGEHFNTAEIVEGSMSLE
                     DMEKEMINKALKKHNSKRKEAADDLGISERTLYRKIKQYDIPL"
     gene            2053..2460
                     /gene="lptE"
                     /locus_tag="PLE23_06180"
     CDS             2053..2460
                     /gene="lptE"
                     /locus_tag="PLE23_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HMX82888.1"
                     /translation="MNTFFVENTQSSVSAVPATFPVGFTEDLKNKIRNESRLKYTETD
                     PDVEFVPSIQSFQVLAIAPKPGESAAINRLELNIQIEYINHKNEKDNWKQNFRHFADF
                     PSSENLLNVQDQLLKIISEQLLEDIFNKAFTNW"
     gene            2595..3455
                     /locus_tag="PLE23_06185"
     CDS             2595..3455
                     /locus_tag="PLE23_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82889.1"
                     /translation="MKAQNVENFETALQEEAPLIPDRALLKKRISVISDLQENIENQT
                     FTSEHTINIAEEVKDINEDLNNQQNGDNEDVKSFHLNNDSINIQEQEQQNSETLIDDK
                     NTSALENSPQQTQQSEEEESSTIKSDAMSEESPKQKTEYNDEPLSAFAIWLKNRSVPI
                     EQPVKILKQEETKVEPVQAVITTAAEEKTTNNEWDGIQEKILSQYQTDLSESKSKQKK
                     KVKSTPKPKKKKENNTGDETTFITETYALLLEKQQKYSQALKIYDKLSLLFPEKRHYF
                     AQKIELLKNL"
     gene            3459..3722
                     /gene="secG"
                     /locus_tag="PLE23_06190"
     CDS             3459..3722
                     /gene="secG"
                     /locus_tag="PLE23_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMX82890.1"
                     /translation="MLTFLVIITAVICVLLMLVVLIQNPKGGGIDSTFGGNATNQLFG
                     ASRSTDFIEKLTWYLAGALFLLCIVIAIMVQSSGGSGIQLMTQ"
     gene            3997..4995
                     /gene="aroF"
                     /locus_tag="PLE23_06195"
     CDS             3997..4995
                     /gene="aroF"
                     /locus_tag="PLE23_06195"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HMX82891.1"
                     /translation="MILQLHKSISTQEAANLAVELKAFHITTNDNHILITSSGMKEVP
                     VGLENKIEQYWVFNSDMQLSAKSFLPQKRQICIGDKIIGGNSMQTLLIGGPCSVESEE
                     QIRQSAELIMSLGLTTLRGGCYKPRTSPYSFQGLGYDGLMLLVKMKEEYGLTIITEAK
                     DATHIEEVIEHSDIIQIGAKAMYDQGILHACSKTTKPVLIKRGFGSTLQEFVQAAEFV
                     LSGGNENVILCERGIRTFETGTRFTLDLCGVAWLQHHTNLPVILDPSHALGKTYGIPS
                     LARACTAMGIDGLLIETHPNPKVAKSDASQQLTHEEFTQMYHSLKPVAEAVGYKII"
     gene            4999..5673
                     /locus_tag="PLE23_06200"
     CDS             4999..5673
                     /locus_tag="PLE23_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HMX82892.1"
                     /translation="MYLEQNLKGLCQQHKMDYEALLDDLEIDDIEEISLQDLEILCEE
                     YQIDLYALLFKPLFLDKNIRQKLAKIKLLVLDVDGVMTDGGMYFTEKGDHIKKYNAKD
                     GMAIQTLVKRGFQTAIISAGYMQEVVQVRARLLGIQHCHVTQEPKLEVLNRICGDLGI
                     ALDEVALIGDDINDLEMMQNIGFSACPADAVNQIKFQSDIVLSKKGGEACVREFVDAY
                     LMKAIG"
     gene            complement(5675..5965)
                     /locus_tag="PLE23_06205"
     CDS             complement(5675..5965)
                     /locus_tag="PLE23_06205"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HMX82893.1"
                     /translation="MKKRNEVKLKDILNEFRRDHKYEQHLSEIEIREVWEKEMGATIS
                     KYTSKIYFRQGILYVKLDSSVLRHELYSNREMILQRLNDALGKSVVKEISLQ"
     gene            complement(5962..7071)
                     /gene="recF"
                     /locus_tag="PLE23_06210"
     CDS             complement(5962..7071)
                     /gene="recF"
                     /locus_tag="PLE23_06210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HMX82894.1"
                     /translation="MFLEEIKIAGFKNIEHAKLHFNPKINCLTGLNGVGKTNCLDAVF
                     LLCVGKSHFNLTEQQLIKQGGNFFRVEGLFTNDNNDRKKVVIKLQDSGKKNISLDDKP
                     YKKIAEHLGRFPVVMIAPDDTEIICGSSEVRRKFLDTTLCQTDRTYLDALTAYNKILK
                     QRNAYLKMLSASGGADNDLLETYDKQLATPAHIIHDKRMKYTAELDTVFIEKFSSITS
                     GDELVKTYYQSQLSDMSMVDLLANNRRKDLITERTNAGIHKDELQISIETREARKFAS
                     QGQQKSLLFALKFAEYTLLMRHTKVKPILLLDDIFDKLDDIRVRNLLGILKNGIFGQV
                     FITDTSADRIKSVFSGTFDHFSLFAVEHGHLKAES"
     gene            7185..8222
                     /gene="pdhA"
                     /locus_tag="PLE23_06215"
     CDS             7185..8222
                     /gene="pdhA"
                     /locus_tag="PLE23_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HMX82895.1"
                     /translation="MQQVLEKVKAPKGGSKKSSFPKETWLTWYETMLRIRRFEERSLM
                     MYSQQKIRGFCHVYIGQEAIAAGLVSALRKEDALITAYRAHGIALAKGLSSKECMAEL
                     FGKATGCVGGKGGSMHFFSAEHKFFGGNGIVGAQIPIGAGIAFAEKYKGTDNLCVTMF
                     GDGAARQGALYESFNMAMTWKLPALFICENNQYAMGTSVARTSNVQDLYKIGAAFDMP
                     SEAVDGMDPEEVHNALDRAAKHIRSGKGPYFLEIKTYRYKGHSVSDPGKYRTKDELQA
                     YMDIDPLKLTEEKILKNKIATDAEIQSIKDKIKAEIEEATQFAEDSPLPIAADLYTDN
                     YVENDYPYVRD"
BASE COUNT         2806 a         1667 c         1701 g         2335 t
ORIGIN      
        1 ctctacatcg cacccaaagg acattactga tgaggtatta cataccatgg cagcatacca
       61 taatatctgc aattatatac atttgccggt acagtcagga agctccagaa tactcgaact
      121 aatgaaccgt acttatgacc gtgaatggta tatagacaga atagatgcca tcaggaatat
      181 catcccggac tgtgccatct ctaccgatgt catcagtgga ttctgcactg aaacagaaga
      241 agaccatcag gaaaccttgt caattatgga atacgctcaa tattcgatgg cgtatatgtt
      301 cttttactcc gaaaggccgg gtacactggc tgcccgaaag tatgaagatg acatacctga
      361 agaagtaaag aaaagacgcc tcaatgaagt aatagaaatg caaagagcaa tttctctcaa
      421 gcacaacctg aatgatatcg ggaaacgata cgaagtattg atagaaggtg attcgaaaaa
      481 atcaggtgaa cagttcaaag ggcgaaattc gcagaataag atgatagtct ttgacaaaat
      541 cgatggaata aaagccggtg attatacaaa tgttgaggta acagaagcaa cctcagctac
      601 attgctgggc aaaatcattg atatttaata caaaaaccag aaataaccca tacaggatgc
      661 aaaatctgct atccatcaaa caaagattcg gtatcattgg tctgtcaaaa tcgcttgacc
      721 aagccctaga tgtggccgta cgtgtggcag ctaccgacct taccgtgctg ataaccgggg
      781 aatcgggtgt tggtaaggag gttttctcga aaatcatcca ttcgcttagc aaccgcaagc
      841 acaatccttt tatagccatc aatacaggtg ccataccacc cggtaccatc aactctgagt
      901 tgttcgggca tgagaaggga tcgtttaccg gagctacggg cgaccgtaaa ggttattttg
      961 aaaccgtcaa cggaggaacg atttttctgg atgaaatagg cgaaatgccg ctcgacacgc
     1021 aggctttcct tctccgcatt cttgaatcgg gtgaatttat aagggtgggt tcttctgcgg
     1081 tacacaagac agatgtacgg gtaatagctg ctaccaatgt tgatttgacc cagaaggtaa
     1141 aggaaggaaa atttagggaa gacctttatt acaggctcaa taccgtaccc atacgcgtac
     1201 cttcgcttaa ggaacgcaag gaagacatcc ctatgctgtt caggaaattt tgtatagatt
     1261 tttctgaaaa atacaggtct tcagtagtac aacttgacga agaagctact aatatactca
     1321 tcaattattc atggccggga aatatccgcg agcttaaaaa tgtagccgaa caggtatccg
     1381 tacttgcaaa caacaagatt gtcaccagcg aggatttggt ctcgtttgta ccaaacttac
     1441 tcgtacgcaa tttgcctgca ttttcgccgg accttggcaa tagctcttcc tccggtgggt
     1501 actacgaacg cgaactactc tacaagctct tgttcgaaat gaaaaaagat atcagtgacc
     1561 tgaaaggatt gatatttgag ctcatcaatg cgaatgacct aaacatttct gatgtcaatt
     1621 ctttggtttc gcttcaagct gaaagttacc cgagatataa tgcagatgta gcatacccaa
     1681 cggacagaca aggcccgtca ttcaatccac agcctgtaaa atacgctccc gaagcaggta
     1741 tggaagaaaa gcccaatacg ctggtcatca accatcctaa cggcgaacat tttaatactg
     1801 ctgaaatagt agaaggaagt atgtcactcg aagacatgga aaaagagatg ataaataagg
     1861 cactaaaaaa gcacaacagc aagcgcaaag aagcagctga tgacctggga atttcagaac
     1921 gcacattgta cagaaaaatt aaacagtatg acattccact ttaaatatta tggcaaatgg
     1981 ttaattttga caatgctttg cctgcatttt ttgggatgtt attcctttaa aggcatttca
     2041 atacctgaaa atgtgaatac attttttgta gagaatacgc agtcttctgt gagtgctgta
     2101 ccggctacct tcccggtcgg attcaccgaa gatctgaaga acaagatcag gaatgaatct
     2161 cgcctgaaat acacagaaac tgacccggac gttgaatttg tgccgtccat acagagtttt
     2221 caagtactcg ccattgctcc aaaaccgggc gaatcggcag ccatcaacag actggagctt
     2281 aacattcaga tagaatacat caaccacaag aatgaaaaag ataactggaa gcagaatttc
     2341 aggcattttg ccgattttcc ttcatcagaa aacctgctga atgtacagga tcagttatta
     2401 aaaatcattt ctgaacaatt actggaagat attttcaata aagcattcac aaattggtaa
     2461 attgcagcat gattacagat aagatatatt cactggacct ttcgtatctt caatcgcttc
     2521 agctaaccga gatacagcaa aacatcaaaa actatccgtt ttcggtcaat atgcgaatac
     2581 tgcgattgct tgccatgaag gctcagaatg tcgagaattt tgaaactgca cttcaggaag
     2641 aagctcccct tatacctgac agagccctgc taaaaaaacg gatatcagta atctctgatt
     2701 tgcaagaaaa tatagaaaac cagacattta cttcagaaca taccatcaac attgcggaag
     2761 aagttaagga cataaatgaa gatcttaata accaacaaaa tggtgataat gaagatgtta
     2821 aatcgtttca tttaaacaat gactctataa atattcaaga gcaagaacag cagaatagcg
     2881 aaacacttat tgatgacaaa aatacttcag cattagaaaa ttcgccacag caaacacagc
     2941 aatcggagga agaagaaagt tcaaccataa aatcggatgc catgtcagaa gaaagcccta
     3001 aacagaaaac cgaatacaac gatgaacctc tctcagcgtt tgctatttgg ctaaaaaaca
     3061 ggtctgtacc aattgaacag ccagttaaaa tattaaaaca agaagaaaca aaagttgaac
     3121 cggtacaagc agtaataacc acagcagcag aagaaaaaac tacaaataat gagtgggacg
     3181 gtatccagga aaaaattctt tctcagtacc agacagacct gagtgaatcg aaatcaaaac
     3241 aaaagaaaaa agtaaaaagc acacctaagc cgaagaaaaa gaaagaaaat aacacaggcg
     3301 atgaaactac ttttatcacc gaaacgtatg ctttactgtt agagaaacaa caaaaatatt
     3361 ctcaggcatt aaagatttat gacaagctaa gtttgttatt cccagaaaaa agacattact
     3421 ttgcgcaaaa aattgaacta cttaaaaact tgtaaaatat gttaacattt ttagtgatta
     3481 ttactgccgt catttgtgta ttgcttatgt tagtggtttt gattcagaat ccaaaaggtg
     3541 gaggaattga ttctactttc ggtggtaatg ctaccaacca gttattcgga gcttcacgtt
     3601 ctacagattt tattgaaaaa ttgacctggt accttgccgg tgctttgttt ttgctatgca
     3661 tcgttattgc cattatggtt caatcatctg gtggttcagg aattcaactt atgactcaat
     3721 aatttccttg aaaaaaaata ctttaaagag ccggatcaga agatttggct cttttttttg
     3781 cattcatatt tttaccatag aacctattca tttatgctaa aaaaacaccg gaaaataaaa
     3841 catccataat cagtttgggt ttttttttgg agtattcgga tggaaatgtc atctttgtac
     3901 tactgtagcg gtttcagtta aacaatgtgt tgcagtaatt aattttagaa ttcttatgtt
     3961 taaacggccg gaaagcaggt acggcattaa aataaaatga ttttacaact ccataaatca
     4021 ataagtactc aggaggcagc caacctggca gtagaactga aggcctttca tataactacg
     4081 aatgataatc atatactgat tacgtcttcc ggtatgaaag aagtacccgt tgggttggaa
     4141 aacaaaatag aacaatactg ggtttttaac tccgacatgc agctttctgc aaaatctttt
     4201 ctaccacaaa agagacaaat ctgcataggt gataaaatta ttggtggcaa ctccatgcaa
     4261 actttgctca tcggtggccc atgctctgta gaatctgaag agcagatacg acagtcggca
     4321 gaattaatta tgtcacttgg cttaacaaca ctacgaggtg gctgctacaa accgcgcacc
     4381 agcccctact ctttccaagg actcggttat gacgggctta tgctattggt aaaaatgaag
     4441 gaagaatacg gtcttactat tattactgaa gcaaaagatg ccacccatat tgaggaggta
     4501 atcgaacatt cggacattat acagataggt gcgaaagcta tgtacgacca gggcattctg
     4561 cacgcatgca gcaagacaac caagccggtt cttataaaac gcggattcgg ctccacattg
     4621 caggaatttg tacaagcagc agaatttgta ttgtcgggtg gcaatgagaa tgtcatattg
     4681 tgtgaacgag gcatacgcac cttcgagaca ggcacaagat ttacgcttga cctctgtggc
     4741 gttgcttggc tgcagcatca caccaatctt ccggtaatac ttgacccttc tcatgcactt
     4801 ggcaaaacct atggtattcc ttcccttgcc cgagcttgta cagctatggg tattgatggc
     4861 ctgctgatag agacccaccc caacccgaaa gtcgcaaagt cagatgcatc gcagcagctc
     4921 acgcacgaag aatttacaca gatgtaccat tcactcaaac cggtagcaga agcagtcggt
     4981 tataaaataa tataaaatat gtatcttgaa caaaacctca aaggtctttg ccaacaacac
     5041 aaaatggact acgaagcctt actcgatgat cttgaaatag atgatattga agaaataagc
     5101 cttcaagatc tggaaatact atgtgaagaa tatcaaatag atttgtacgc actattgttc
     5161 aagcccttgt ttctggataa aaatattaga caaaaacttg caaaaattaa acttctggta
     5221 ctagatgtgg acggagtaat gaccgatggt ggcatgtatt ttactgaaaa aggtgatcat
     5281 ataaaaaagt acaatgccaa agacggaatg gctattcaga ccctcgtaaa acgaggattt
     5341 cagactgcca tcatatcggc cggatatatg caggaagtag tacaggtacg cgccagacta
     5401 ctgggcatac agcattgtca cgtcacccag gagccaaagc tggaagtcct gaacagaata
     5461 tgcggtgatc ttggtatagc acttgacgag gtagccttga taggtgacga catcaatgat
     5521 ctcgaaatga tgcaaaacat cggattctca gcctgccctg ctgatgcagt caaccagata
     5581 aagttccagt ccgatattgt gcttagtaaa aaaggcggtg aagcttgtgt ccgcgaattt
     5641 gtagatgctt acttaatgaa ggctatcggt tagtttattg aagactaatt tcctttacta
     5701 cacttttgcc aagtgcatca ttgagccgtt gaagaatcat ttcccgattg gagtataatt
     5761 cgtgccttag tacagaactg tcaagcttta cgtaaaggat tccttgcctg aagtaaattt
     5821 ttgaagtata tttgctaatg gtggcaccca tttctttttc ccacacttcc cttatttcaa
     5881 tctcagacaa atgttgttca tatttatggt ctcttctgaa ttcgttcaga atgtccttca
     5941 gttttacttc attccttttt ttcatgattc agcttttaag tggccatgtt ctactgcgaa
     6001 taagctaaaa tgatcaaaag taccggagaa aacacttttt attctgtcag cagaagtgtc
     6061 ggtaataaaa acctgcccaa atataccatt tttcagtatg cctaacaaat tccttacccg
     6121 tatgtcatcc agtttgtcga aaatatcatc aagcaaaaga atgggcttca cctttgtatg
     6181 gcgcatcaac aaggtgtatt cggcaaattt tagtgcaaac aaaagtgatt tttgttggcc
     6241 ctgtgatgca aactttctcg cctctcttgt ttcgatagaa atctgtaatt cgtccttatg
     6301 aatacctgca ttggtacgct ccgttatcag gtcttttctg cggttgttgg caagcaaatc
     6361 taccattgac atatcgctca actgcgattg gtagtatgtc tttaccagct cgtcgcctga
     6421 agtaatgctt gaaaattttt cgataaatac cgtatccagc tctgctgtat atttcattct
     6481 tttgtcatga ataatatggg caggggtggc caactgttta tcgtaggttt caagcaaatc
     6541 attatctgca ccaccagaag cagagagcat tttgaggtaa gcatttcgct gtttgagtat
     6601 tttattgtaa gcagtaagag catcgagata tgttctgtca gtctggcaaa gtgtagtatc
     6661 gagaaatttg cgccttactt cagaagatcc acagatgatt tcagtatcat caggagcaat
     6721 cataaccacc ggaaaacgtc cgaggtgttc agcaattttt ttgtatggct tgtcatcgag
     6781 tgaaatattt tttttgcccg aatcctgaag ttttataact actttttttc tgtcgttatt
     6841 atcatttgta aataaacctt ctactcggaa aaaattgccg ccttgtttta tcagttgctg
     6901 ttcggttaag ttaaaatgac tcttgccgac acaaagcaga aaaacagcat cgagacaatt
     6961 ggttttccct acaccattca agcctgtgag gcaatttatt tttggattga aatgcaattt
     7021 tgcatgttca atattcttaa aacctgcaat tttaatttct tctaaaaaca ctcgatttat
     7081 tttacaaaca caaagatgaa ataaatttca gtactacagt tgctttcaga tttggataat
     7141 cataaaacaa gtcgtatttt tatgctttca aatcaatatc aattatgcaa caagtactcg
     7201 aaaaagtaaa ggcacctaaa ggaggttcaa aaaaatcatc ttttccgaaa gaaacatggc
     7261 tcacttggta tgagactatg ctccgtatcc gaaggtttga agagagaagt cttatgatgt
     7321 attctcagca aaagatccgg ggtttttgtc acgtttatat tggtcaggag gccattgcag
     7381 ccggacttgt aagtgccctt cgcaaagaag atgcgctcat aacggcatat cgtgcacatg
     7441 gtatagcgtt ggcaaaaggc ctgagttcga aggaatgtat ggcagaatta ttcggaaagg
     7501 ctaccggttg tgtaggcggc aaaggtgggt cgatgcactt cttctctgcc gagcataagt
     7561 tttttggtgg taatggcata gtaggggcac agattccgat aggtgccggt attgcatttg
     7621 ccgaaaaata taaaggtact gacaatctgt gtgtaaccat gtttggagat ggtgctgctc
     7681 gccaaggtgc attgtacgaa tcattcaata tggctatgac ctggaaacta cctgccctgt
     7741 tcatctgcga aaataaccag tatgcaatgg gtacttcggt ggcacgtacc agcaatgttc
     7801 aagatctgta caaaataggc gccgcatttg acatgccaag tgaagcagtt gacggtatgg
     7861 acccggaaga agtacacaac gcacttgatc gcgcagccaa acatatcaga agtggtaaag
     7921 gcccttattt cctcgaaata aaaacataca gatacaaagg tcactcagta tctgacccgg
     7981 gcaaatacag aacaaaagac gaacttcaag catatatgga tattgatcca ttaaaactca
     8041 cagaagaaaa aattctgaaa aacaaaatag caaccgatgc tgaaatacaa tcaatcaaag
     8101 ataaaataaa agcagaaatc gaagaagcaa cacagtttgc cgaagattca ccactgccga
     8161 tagcagcaga tttatatact gataattacg tagaaaatga ttatccttat gtaagagatt
     8221 aatatacaag taagaatatt taaatatttt gagtaagcag ttaagagaaa ttttagctgc
     8281 ttttcatttt tattgaaaca ttttataaaa aataaacgta catttgcgga aatttttaca
     8341 gaaagtagat acccattatg gcaaagagaa aagaagacat cacagaaaca ctggtaaacc
     8401 ttgacgaaat ctcggattcc gtaacttcct attatgacaa gtacaaaaac atcatcttgg
     8461 gtgttattgg cggtttggtt gttttgattg gaggttatta tgcttataa
//
LOCUS       DAOQQY010000099         8454 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3242_length_8454_cov_4.782474, whole genome shotgun sequence.
ACCESSION   DAOQQY010000099 DAOQQY010000000
VERSION     DAOQQY010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3242_length_8454_cov_4.782474"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2373)
                     /locus_tag="PLE23_06220"
     CDS             complement(<1..2373)
                     /locus_tag="PLE23_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX82896.1"
                     /translation="MTKNIFTIALCLLTLGLFGQSQVFSGKLVQTNEYNQLDLFLKHY
                     EIYQIDAVSMHNFAMKNKAENLQFQLNLGTQHSFSLNLQENKIFKEGIQVRVVTENGI
                     EPGTLSKIKCYNGYTAGDHKKVSLSINNNLLFGFITTDEGEYFVEPLWHLIPDAPQDQ
                     FIFYYAKDYIETKEGKCGMTEAEEYGEKLHNKIQNTKGIAEGAPLGCKEVEIAEAADK
                     LMSVKYFDQQGVEDRITSMINLVQANYYGSFNDDLTLIISEFFIVDKNGTDPWTPSTD
                     PSALLSSFRNWGPTNFGQHDVGELFTNRDFDGGTVGIAYLSAVCTSNRYNCIQDWTNN
                     LSLLRVTIAHELGHNFSCQHDGSGTPYIMAPAVNNTNTWSSTSITSFNNFVPSRTCLA
                     ACSGGGLPPEADFSANILEGCKPLTVQFTDNSLHSPTSWNWTFPGGTPSSSNAKNPVV
                     TYNNAGWWDVTLVATNNNGSDTESKQQYIHIKEKPIVSFTYTKLLGNVSFINNSTGGN
                     IFYWEFGDGNFSTDEHPTHDYTDPGSYTVTLTVTNECGSTTLTKVIEIVFIPLANFSS
                     NISSGCVQMFVNFEDESTFNPTSWQWTFPGGVPASSSLQNPVVSYSTPGVYDVTLVAT
                     NSAGSNTITITNYIKALDKPVPSFTQTITGYKVVFNNTTPGTDNTYEWNFGDGTTSTE
                     KNPEHTYGSSGQFTVTLKVTNSCGSTTITKDITILAVPKAQFSSSVTSGCAPLTVNFT
                     DNSTNNPSSWLWSFPGGNPSSSTVQNPTVIYNNPGSYNVTLIASNAIGSDT"
     gene            complement(2497..5880)
                     /locus_tag="PLE23_06225"
     CDS             complement(2497..5880)
                     /locus_tag="PLE23_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX82897.1"
                     /translation="MIRKLLSVLICFASITLFGQTAAFVGKPVGKTEYSELNEFLSDY
                     EIYSIDATSIYNYVKKSKTEHTEFQLNLGDKHSFKLDLQENIIQRPGIKAKVLTENGI
                     ELQEPSGIKCYNGVVAGTTRKASISVDKEFLLGFVVDNNEDFWFEPLWHIIPGAPTNQ
                     YIFYYAKNFKQTSEHLCGAVEANNKHKQIENQIKATKGTPESPLGCKEVELAEASDKG
                     MCSKYMSISGAQNRIISVINTVQNNYNGWFDDNLTLKIVDWYNVACNGSDPWSNTVDA
                     EQLLNEFTDWAPGNFSDHDIGQLWTSRTFSFGVVGIAWLGTVCTNLRYNCISDFTSNP
                     NFIRCTVSHEMGHNFNCEHDASGAPYIMAPAVSSTNNWSSESVNTFNAFIPSRWCLSA
                     CTGGNPPSADFYSDEQTGCKPFTVHFTDNSTGDPTQWTWTFSGGTPSSSTLQNPTVVY
                     NNPGIYDVTLKATNASGSDTYTQAKYITVRDIPVADFSVTSIQGLAIFDNKSIDGEDY
                     YWEFGDGEDSAEEEPTHDYAEPGTYTVSLTVTNDCGSSTYIKVIEIVFVPLADFSSNI
                     SKGCVPMVVNFTDESEYEPTSWQWTFPGGTPSSSTLKNPTITYNTPGLYSVTLVATNS
                     AGSNTITIDKYISAATVPVASFTDTIAGNTVSFNNTTQGNDNQYLWTFGDGDSSMVKN
                     PVHLYDQSGTFTVTLYVTNTCGSDTITGVITIVAKPEAGFTSDKSKGCIPFVVHFQDN
                     STNQPTSWQWSFPGGSPSTSNAQNPIITYPATGSYEVSLIAGNIAGSDTIVIQKYINV
                     NDKPLASFSYSIDKNVVTCLSNSTNATSWLWKLSDGNTYTTENITHTYLTSGSKTITL
                     IASNECGSDTTVQTIDIIYDQLTPVAKFEASLASGCLPLLVTFTDFSENNPIEWYWTF
                     EGGDPSTSSVQNPVVIYNTAGEYDVQLIVKNLFGYDTINKPDYIKVTGLPAVKFTYSL
                     KDGIVSFVNQTIGGTAYLWNFGDGTTSTEENPVHTYTVPGEYTVSLTVTNECGSFTFN
                     QKINTMVGSKDIDFVQSLILYPNPNAGRMTIEIHSLASRHLDIKLADVLGREINQTEI
                     QLSAGVNKTELNPDGILPGTYFVKIESTDGMIVRKMLVK"
     gene            6134..6787
                     /locus_tag="PLE23_06230"
     CDS             6134..6787
                     /locus_tag="PLE23_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82898.1"
                     /translation="MQKKFQFLILAIVCLFVLLPNTTFAQEKSKKNVDKYFDESGGFK
                     HRLWYGGGFNLGFSGDNAYSQFNIGITPMVGYKVLPSLSFGPRFGFDYTYIKGETIDE
                     TGNPRGRRSANLLNYSVGAFGRFKFLKFLFLHAEANYESDERAYIDQYSRYLVYDSKI
                     NKIYTERIGSENYLAGLGYNSGDGLIGYEIVGLYNFSVPSDSPALPFEFRFGFTYKF"
     gene            6874..>8454
                     /locus_tag="PLE23_06235"
     CDS             6874..>8454
                     /locus_tag="PLE23_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82899.1"
                     /translation="MFAQNLKQVTVKISQTGNTIVSDSLPFFPNSIDISCTPSGTKLE
                     ISDFLVDGKEIKIFPGKSLEHDTAYIVRYRQFDLAVFRPLRLVDTSEISNQKENPFLA
                     YQFSEDKKTDTGFGNLNYRGSISRSILAGNNQDLSLNSTLNLQFSGKIDDETEIAAVV
                     TDNNIPIQPDGATRQIQEFDKIYIQVKRRNSSLLAGDYEINAPEGYFMKYFKKLRGAT
                     ISNTTKSGNKTLNSKASFAISRGKFSRMVINGIEGNQGPYKLNGSGGESFIIILSGTE
                     KVFVDGMVMQRGLQDDYTIDYNRGDITFTPRRMITKDSRIIVEFEYVVQNYVRSIATI
                     SETVKINKSTVSVNLYTEQDHKNSSQTPLTTLEKTALSQAGDNTSLAIVASIDTSNAD
                     PVKYRLTDTLGFTSVLVYSPNNPNAKYTASFAFVGNGNGDYILDTEKGVNGRVYKWVA
                     PNTNGTKNGNYSPVRKLIAPTLQQMATVSIATPIFKKILVNGELAYTRLDKNRFSDND
                     NSDDNGFGTKFSLTRQDSI"
BASE COUNT         2528 a         1681 c         1652 g         2593 t
ORIGIN      
        1 tgtatcactt ccaatagcgt tacttgctat caatgtaaca ttgtaacttc caggattatt
       61 atagattacg gtaggatttt gcacagtaga acttgaagga ttaccacccg gaaatgacca
      121 aagccatgaa gaaggattat tggtagaatt atcagtaaaa ttaactgtca atggcgcaca
      181 acccgatgta acggaagagc tgaactgagc tttgggtact gccagaatag tgatgtcttt
      241 tgtaatagtg gtagagccac aactgttggt aactttcagt gtgactgtaa actgacctga
      301 agaaccgtag gtatgttccg gatttttttc ggtgcttgta gtgccatcac caaaattcca
      361 ctcataagta ttatctgtgc cgggtgtcgt attattgaac acaaccttat agcctgtaat
      421 agtttgggta aaagatggta caggcttatc aagcgctttg atataattgg taatggttat
      481 agtattgctg ccggcgctat tagtagccac caatgtaacg tcatacactc ctggcgttga
      541 ataggagacc acaggattct gaagtgaaga acttgcaggt acgccaccag ggaatgtcca
      601 ttgccatgac gtaggattaa aagtactttc atcttcaaaa ttgacaaaca tctgaacaca
      661 gcctgacgaa atatttgagc taaaattagc cagcggaata aatacaattt cgatcacctt
      721 tgtaagagta gtagatccgc attcatttgt aacggtaagc gtcacagtat aactaccggg
      781 gtctgtataa tcatgtgtcg ggtgttcgtc ggtagagaaa tttccatcac cgaattccca
      841 gtaaaagata ttaccaccgg ttgaattatt gataaaactc acatttccta aaagtttggt
      901 atatgtaaag cttacaatag gcttttcttt aatgtgaata tattgttgct tcgattctgt
      961 gtccgatcca ttgttattgg tagctacgag tgtcacatcc caccaacctg cattattata
     1021 ggtcacaacc ggattttttg catttgagct tgatggtgtt cctccaggaa atgtccagtt
     1081 ccaagatgta ggactgtgca atgaattatc ggtaaactgt acagtcagtg gtttacagcc
     1141 ttcgagaata ttggccgaaa aatcggcttc aggtggcaga ccaccgccac tgcatgcagc
     1201 caggcacgta cgtgaaggca caaaattatt gaatgaagta atagaagtag atgaccaggt
     1261 attggtgtta ttaacagcag gtgccatgat gtaaggcgtg ccactaccat cgtgttgaca
     1321 actaaaattg tgccccaact catgcgcaat ggttactctc agcaagctta agttgtttgt
     1381 ccaatcctga atacagttat atctattact tgtacaaaca gctgaaagat acgcgatacc
     1441 tacggtgcct ccgtcaaaat cacggttagt aaacaattcg cctacatcat gctgcccgaa
     1501 gttagttggg ccccagtttc tgaaagaact caacaaggct gaaggatctg tactgggtgt
     1561 ccatgggtct gtaccatttt tatcaacaat aaaaaattct gagataataa gtgtaagatc
     1621 atcattgaat gaaccataat aattggcttg cacgagattt atcatggaag taatccggtc
     1681 ttctacacct tgttggtcaa agtattttac actcatcagc ttatcagctg cttctgctat
     1741 ctctacttct ttgcaaccta gaggagctcc ttcagctata cctttggtat tttgtatttt
     1801 attgtgcaat ttttctccat actcttctgc ctctgtcatc ccacatttgc cttccttagt
     1861 ttcgatataa tcttttgcgt agtaaaaaat aaactgatcc tgtggcgcgt cgggtatcag
     1921 atgccaaagt ggttcaacga agtactcacc ttcgtctgtg gttatgaagc cgaaaagcag
     1981 attattgtta atcgatagac ttactttttt atgatcaccg gctgtatatc cattataaca
     2041 tttgatttta ctcagtgttc cgggttctat tccattttcg gtcacaaccc gtacctgaat
     2101 cccttccttg aaaatcttat tctcctgaag attgagtgaa aacgaatgct gcgttccgag
     2161 gttgagttga aactgcagat tttcagcttt gttcttcatt gcaaaattat gcattgacac
     2221 agcatctatc tggtaaatct cataatgttt taaaaacaaa tcgagctgat tgtactcatt
     2281 cgtttgtaca agcttacccg aaaatacctg cgattggccg aacaaaccca gtgtcagcag
     2341 gcataatgca attgtaaaaa tattttttgt catggtgtgt tggggtggtt taattcaaat
     2401 atattgatta atattacatc taaatataac aaactcgtca attgtttaat taatttggtt
     2461 aattatttca attgttcctt atttttgtct gacaatttac ttcacaagca tttttctcac
     2521 gatcattccg tcagtggatt cgatttttac aaaatatgta cccggcaata ttccatccgg
     2581 attcagttca gtcttgttaa ctccggcaga cagttggatt tctgtctgat tgatttcgcg
     2641 accaaggaca tctgcaagct ttatatcaag atgccttgaa gctaaagaat gtatctcaat
     2701 ggtcatgcgt ccggcattcg gattcggata gagtatcagg ctctggacaa aatcaatatc
     2761 cttcgaccct accatggtat taattttctg attaaatgta aatgagccac attcgttagt
     2821 aacagtgagt gaaacagtat attcacccgg cactgtataa gtatgtaccg gattctcctc
     2881 agtagatgtt gtaccgtctc caaagttcca cagatacgcg gttcctccta tagtctgatt
     2941 gacaaaactt actataccat ccttgagtga ataagtaaat ttaactgcag gcaatcctgt
     3001 caccttaatg taatcaggtt tattgattgt atcataaccg aacaggtttt tcactataag
     3061 ctgtacgtcg tactcaccag ccgtattata aatgacaaca gggttttgaa cacttgatgt
     3121 tgacggatct cctccttcaa aagtccagta ccactctatc ggattatttt cagaaaaatc
     3181 agtaaatgtt accagtaatg gcaaacaccc actcgccaat gatgcttcaa attttgcaac
     3241 aggtgtcaat tggtcataaa taatgtcgat cgtctgtact gtggtatcac taccgcattc
     3301 attggaggca atgagcgtta tggtctttga cccgcttgtc aggtaggtat gtgttatatt
     3361 ttcagtagta taagtgtttc cgtcacttaa tttccataac catgatgtag catttgtaga
     3421 attgctcagg caagtaacaa catttttatc tattgaataa ctaaagcttg ctaatggttt
     3481 gtcattgaca ttgatatatt tctggatgac gattgtatcg ctgcctgcaa tatttccggc
     3541 tatcaaactt acctcatagc tgccagttgc cgggtaggtg atgattggat tttgagcgtt
     3601 tgaagtagaa ggagaaccac ccgggaaact ccattgccag gaagttggtt gattggttga
     3661 attatcctga aaatgaacga caaatggtat gcagcccttt gatttatctg aagtaaaacc
     3721 agcttcaggt ttagctacta tggtaatcac ccctgtaatg gtgtcactgc cacaagtatt
     3781 tgttacatat aatgttacag taaatgtacc tgactggtca tacagatgaa ccgggttctt
     3841 gaccattgac gaatcaccat caccgaatgt ccacaggtat tgattatcat tgccttgtgt
     3901 tgtgttattg aaactaacgg tattgccggc aatggtatca gtgaatgaag caaccggaac
     3961 cgttgcggca cttatatatt tatcgatagt aatggtgtta ctaccggcac tgttggttgc
     4021 tacaagtgtc acactgtata atccgggagt attataagtg attgtcggat tcttcaatgt
     4081 agaactggat ggcgttcctc cggggaatgt ccattgccag gaagtaggtt catattcact
     4141 ttcatccgta aaattaacca ccattggtac acagccttta ctgatattag agctaaaatc
     4201 agccaatggt acaaatacta tttcaattac tttgatgtaa gtagaagaac cgcagtcatt
     4261 ggtcaccgta agcgatacag tatatgtacc gggctctgca tagtcatgag taggttcttc
     4321 ttcagccgaa tcttcaccat ctccgaactc ccagtaatag tcttcgccat caatactttt
     4381 attgtcaaaa atagctaaac cctgaatgct tgtaacacta aaatcagcta ccggaatatc
     4441 tcttactgtg atatattttg cctgtgtgta tgtatcagag ccggaagcat tcgttgcttt
     4501 tagcgtaaca tcatagatgc caggattatt atagactacc gttggattct gaagagtgga
     4561 gcttgatggt gtccctcccg agaatgtcca ggtccattgt gtaggatcac ctgttgaatt
     4621 atctgtgaaa tgtacggtaa atggtttgca gccagtctgc tcatctgaat aaaaatcagc
     4681 agaaggagga ttgcccccgg tacaggccga caagcaccac cttgaaggga taaaggcatt
     4741 gaatgtattg acagattcac ttgaccagtt gttggtagaa ctcacagccg gagccattat
     4801 ataaggtgca cccgatgcat catgctcgca gttaaaattg tgccccattt cgtgagatac
     4861 agtacaacgg ataaaattcg ggttacttgt aaaatcagaa atgcagttgt acctaagatt
     4921 ggtgcacact gtacctaacc atgcaatacc caccacacca aaagaaaacg ttctacttgt
     4981 ccataattgc ccaatgtcgt ggtcactgaa gttgccaggt gcccaatcag taaattcgtt
     5041 aagtagttgt tcagcatcta ccgtatttga ccatgggtca cttccattgc aagctacgtt
     5101 ataccagtca actattttta gtgtaagatt atcatcaaac catccgttgt aattattctg
     5161 tacagtattg attacactaa tgatacggtt ctgcgctcca cttatcgaca tatatttgct
     5221 acacatgccc ttgtcagatg cttctgctaa ttctacttct ttgcatccta agggcgactc
     5281 tggtgttcct ttcgttgcct taatctgatt ttcaatctgt ttatgcttgt tgtttgcttc
     5341 taccgctcca caaagatgct cgcttgtttg tttaaaattt ttggcataat aaaagatgta
     5401 ttggttggta ggtgcacccg gtataatatg ccacaatggc tcaaaccaaa aatcttcgtt
     5461 attgtccact acaaaaccaa gaaggaattc tttatccaca gatatacttg cttttctagt
     5521 ggtacctgct actacaccgt tatagcattt aataccgctt ggttcctgca attcgatacc
     5581 gttttcagtc agtacttttg ccttgatgcc cggtctttgg atgatgttct cctgcagatc
     5641 gagcttaaag ctatgcttgt caccaagatt taattgaaac tccgtatgtt cggtttttga
     5701 tttcttgaca taattataga tagaagtagc gtcaatactg taaatctcat aatcggagag
     5761 aaattcattc agttcactgt attctgtttt gcctacaggt tttcctacaa atgccgctgt
     5821 ttgcccaaat aaagtaatag acgcaaaaca aattaaaaca ctaagcaatt ttctgatcat
     5881 acaaaaggct aattataatt aaacaaaatt aatataaaat aaatatatat tagaccaaaa
     5941 ccaacacaaa tttaagcatt tacgatatat tatctgcaaa gttttatgct cgtttttaaa
     6001 taattttcaa attaaattca aacagataaa ttacattctt aaaaaaatag cttgatataa
     6061 aaattcaaat gaatcagttt ttgagcaatg atttataaat ttgcatcgtt atttatatac
     6121 acaattattt tttatgcaga aaaaatttca gtttttgata ttggctattg tttgcctttt
     6181 cgttttactt ccgaatacaa catttgccca agagaagagt aagaagaatg ttgacaaata
     6241 ttttgacgaa tcgggtggat tcaagcatcg cctttggtac ggtggcggct tcaacctcgg
     6301 tttttcaggt gataatgcat acagtcagtt caatattggc attaccccaa tggtgggata
     6361 taaggtactt ccttcgctga gttttggacc acgatttggt tttgattata cttacataaa
     6421 aggtgaaacc attgatgaaa ccgggaatcc gagaggtcgc aggtcagcta atctgctgaa
     6481 ttattcggtt ggtgcttttg gtaggttcaa atttctcaaa tttttatttc ttcatgctga
     6541 ggcgaattat gaaagtgacg aacgtgccta tattgaccag tattcacgtt atctggtata
     6601 tgacagcaaa attaataaaa tatatactga gcgaatcgga tctgaaaatt atctagcagg
     6661 attgggttat aattcaggag atgggctgat aggatacgag atagtgggtc tctacaattt
     6721 ttctgtacct tcagattctc ctgcattgcc atttgagttc aggtttggct ttacttataa
     6781 attctgatca acacaggaag caatcaatgt cagcgtgtga taaaatttcg gttcatattg
     6841 gctttttgtt tactaatggg caatatgaat ctgatgtttg cccagaatct gaagcaggta
     6901 actgttaaaa tatcgcaaac cggcaacact atagtgtcgg atagtctgcc attttttccc
     6961 aacagcattg acattagctg tactccttcc ggtacgaaac tagaaatatc agacttcctt
     7021 gttgatggca aagaaattaa aatattcccg ggcaaaagtc ttgaacatga tacggcttac
     7081 atagtccgtt acaggcagtt tgaccttgcc gtattcaggc cattgagact cgtggatacg
     7141 agtgaaatat ccaaccaaaa ggaaaatcca tttctggcat atcaattttc tgaagacaaa
     7201 aaaaccgaca ccggctttgg caatctcaat tatcgtggaa gtataagccg ttcaatactt
     7261 gcaggaaata atcaggatct cagcctaaac tctaccctca atctgcagtt cagtggaaaa
     7321 attgatgacg aaaccgaaat agctgcagta gtaaccgaca acaacatccc gatccagcct
     7381 gacggggcaa ccaggcagat tcaagaattt gataaaatct atatacaagt aaaacgcagg
     7441 aattcttcct tgctggcagg tgactacgag atcaatgcac ccgaaggata ttttatgaaa
     7501 tattttaaaa aactcagagg cgccaccatc agcaatacaa caaagtcagg aaataaaaca
     7561 cttaactcaa aggcgagctt tgccatcagc cggggcaaat tttcaaggat ggtcatcaat
     7621 ggtatcgaag gcaatcaagg cccatacaag ctcaatggct cgggaggcga aagtttcatc
     7681 atcatcctga gcggtaccga aaaagtgttc gtagatggaa tggtcatgca gcgtggcttg
     7741 caggatgatt atacgattga ttataaccgt ggcgatataa cattcacacc aagaagaatg
     7801 atcaccaaag acagccgaat aatagttgaa tttgaatatg ttgtacaaaa ctatgtacgt
     7861 tctattgcaa cgatttctga aacggtcaaa attaacaaat cgactgtaag tgttaatctc
     7921 tacacagagc aagatcataa aaattcttca caaacacctc ttacaacgct cgaaaaaaca
     7981 gcattgtcac aggcaggtga caatacaagc cttgcaatag tagcatccat tgacacatca
     8041 aatgccgacc ctgtcaagta ccgacttacg gatacacttg gtttcacttc tgtactggtt
     8101 tattcgccca ataacccaaa tgcaaagtac acagcaagtt ttgcatttgt aggtaatggc
     8161 aacggagact atattctcga taccgaaaaa ggggtcaacg ggcgtgtata taaatgggtg
     8221 gcacccaata cgaatggtac aaaaaacggt aactattctc cggtaagaaa gctgattgcc
     8281 cctacccttc aacagatggc aactgtttcg attgctacac cgatcttcaa aaaaatacta
     8341 gtgaatggcg aacttgctta taccaggctt gataagaatc gtttctcgga caatgacaat
     8401 agtgacgata acggatttgg gactaaattt tctttaacac gacaggattc tata
//
LOCUS       DAOQQY010000100         8355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3312_length_8355_cov_7.000964, whole genome shotgun sequence.
ACCESSION   DAOQQY010000100 DAOQQY010000000
VERSION     DAOQQY010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3312_length_8355_cov_7.000964"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..199
                     /locus_tag="PLE23_06240"
     tRNA            117..199
                     /locus_tag="PLE23_06240"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:152..154,aa:Tyr,seq:gta)
     gene            234..306
                     /locus_tag="PLE23_06245"
     tRNA            234..306
                     /locus_tag="PLE23_06245"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:267..269,aa:Gly,seq:tcc)
     gene            343..414
                     /locus_tag="PLE23_06250"
     tRNA            343..414
                     /locus_tag="PLE23_06250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:375..377,aa:Thr,seq:ggt)
     gene            474..1670
                     /gene="tuf"
                     /locus_tag="PLE23_06255"
     CDS             474..1670
                     /gene="tuf"
                     /locus_tag="PLE23_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HMX82900.1"
                     /translation="MAKETFDRSKPHLNIGTIGHVDHGKTTLTAAITNVLAERGLAEK
                     KDYDSIDAAPEEKERGITINTAHVEYQTEKRHYAHVDCPGHADYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVGVPNLVVFMNKVDLVDDPEMLELVEMEVRELLSS
                     YGFDGDNAAVIKGSALKALEGDPKYVQAILDLMDACDSEIKEPTRDIDKPFLMPIEDV
                     FSITGRGTVATGRIERGVIEVGNPVEIVGMMAEGEKPLTSVCTGVEMFRKLLNRGEAG
                     DNAGLLLRGIDKEAIRRGMVICAPGSVKPHRKFKCEVYVLSKEEGGRHTPFFNGYRPQ
                     FYFRTTDVTGDCKLPEGVEMVMPGDNVSLEVILINPIAMEKGLRFAIREGGRTVGAGQ
                     VTEILD"
     gene            1746..1818
                     /locus_tag="PLE23_06260"
     tRNA            1746..1818
                     /locus_tag="PLE23_06260"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1779..1781,aa:Trp,seq:cca)
     gene            1847..2038
                     /gene="secE"
                     /locus_tag="PLE23_06265"
     CDS             1847..2038
                     /gene="secE"
                     /locus_tag="PLE23_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HMX82901.1"
                     /translation="MDKIKLYLSQSYDELVNKVSWPSTNNLIESAKVVLTFVIISTIL
                     VFIMDVISQQALSFIYSIG"
     gene            2074..2619
                     /gene="nusG"
                     /locus_tag="PLE23_06270"
     CDS             2074..2619
                     /gene="nusG"
                     /locus_tag="PLE23_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HMX82902.1"
                     /translation="MSEEKKWFSLRVISGKEKSIRDRIDREIDSCGWRNIVTQVVVPS
                     EKVYKIRNGKKVITERNILPGYILVEADAEKFTAEIVQGIANLPNVINFLGANKPIPM
                     QNAEANRLLGKMDESSEIGESNLEPFIVGETVKIIDGPFFEFVGDILEVNEEKKKLKL
                     IVKIFGRGTEVELNFMQVEKT"
     gene            2641..3078
                     /gene="rplK"
                     /locus_tag="PLE23_06275"
     CDS             2641..3078
                     /gene="rplK"
                     /locus_tag="PLE23_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMX82903.1"
                     /translation="MAKEIEYIIKLQVKGGQANPAPPIGPALGSKGVNIMEFCKRFNA
                     KTQDTPGKIIPVVIEVYKDKSFEFVTKTAPATVQLFEAAGIKKGSAQSNRIKPGSVTW
                     DQIKAIAEDKMPDLNAFKLESAMKMIAGSARSIGLEIKGDAPF"
     gene            3162..3854
                     /gene="rplA"
                     /locus_tag="PLE23_06280"
     CDS             3162..3854
                     /gene="rplA"
                     /locus_tag="PLE23_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMX82904.1"
                     /translation="MAKLSKKRAAIAAKVDQDKLYTLPEAMNLVKEVNTTKFDASVDV
                     HIRLGVDPRKADQALRGTVTLPHGTGKTKRVLVLCTPDKAAEATAAGADHVGLEDYIQ
                     KIAGGWTDVDVIVAMPTVMAQLGKIGRILGPRGLMPNPKTGTVTMDVASAVQDVKGGK
                     ISFRVDKYGIVHSSVGRVSFSPEKLEGNANELISTLVKMKPSSAKGTYLKSISVAGTM
                     SPGIKVDPKTIK"
     gene            3878..4408
                     /gene="rplJ"
                     /locus_tag="PLE23_06285"
     CDS             3878..4408
                     /gene="rplJ"
                     /locus_tag="PLE23_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMX82905.1"
                     /translation="MNKSNKGVAIEELKEKLETANFFYITDSSSLSVEKINNLRRICF
                     NKGVEMKVVKNTLLRKAMESFGEERGYQGLFDALHGPTAIMFTETANVPAKIIKEFRK
                     DDKRPIIKAAYIDTAIFMGDQSIEDLVNLKSKEQLIGEVITILQSPAKNVISALKSGG
                     QTIAGLVKALEERAEQ"
     gene            4482..4862
                     /gene="rplL"
                     /locus_tag="PLE23_06290"
     CDS             4482..4862
                     /gene="rplL"
                     /locus_tag="PLE23_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HMX82906.1"
                     /translation="MADLKALADQLVNLTVKEVNELATILKDEYGIEPAAAAVAVAAA
                     PAGGGAPEAAAEKTSFDVFMTSAGAGKLNVVKEVKNLLGLGLKEAKDLVDAAPAMIKE
                     GCSKDEAESLKAALEGAGASIELK"
     gene            5062..>8355
                     /gene="rpoB"
                     /locus_tag="PLE23_06295"
     CDS             5062..>8355
                     /gene="rpoB"
                     /locus_tag="PLE23_06295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HMX82907.1"
                     /translation="MANNSAMLYAESKRVNFGKIKYPLTPPDLLDIQLKSFQEFFQLE
                     TTPENRSDEGLFKVFQENFPITDARNIFVLEFQDYFIDPPRYSIDECIERGLTYSVPL
                     KAKLRLSCNDEEHVDFETIVQDVFLGNIPYMTPKGTFVINGAERVIVSQLHRSPGVFF
                     GQSVHPNGSKIYSARVIPFKGAWMEFATDINTVMHAYIDRKKKFPVTTLLRAIGFDTD
                     KSILNIFGLADEVPSTREALEASIGRKLAARVLRTWTEDFVDEDTGEVVTIERNDIIL
                     ERDVVIEADNIDLILNAGLESVILQKENIGEDYSIIFNTLQKDPTSSELEAVQYIYRQ
                     LRGTEPPDDETARGIIDKLFFSDKRYNLGEVGRYKINKKLGIDTSDETLVLTKEDIIL
                     IVKYLVSLINQRAEIDDIDHLSNRRVRTVGEQLYAQFGVGLARMSRTIRERMNVRDNE
                     VFSPVDLINARTLSSVINSFFGTSQLSQFLDQTNPLSEITHKRRISALGPGGLSRERA
                     GFEVRDVHYSHYGRLCTIETPEGPNIGLISTLCVHAKINKMGFLETPYRPVNNGVVEL
                     KSEAIYLSAEGEDYSKIAQANAPLTDVGNFESDKVKCREHGEFPVLGPDEIEYMDVAP
                     NQIVGVSASLIPFLENDDANRALMGSNMQRQAVPLIRPTSPIVGTGLEGKVARDSRML
                     INAEGDGYVEYVDAEEIVIRYDRTDEDQITSFEDNRKTYKLTKFARTNQGTCINLKPI
                     VTKGQRVAKGEILCDGYATDKGELALGQNLKVAFMPWKGYNFEDAIVLSERVVKEDIF
                     SSIHIEHFEMDVRDTKLGEEELTNDIPNVSEEATKDLDENGIIRVGAWVKDGDILIGK
                     ITPKGESDPTPEEKLLRAIFGDKAGDVKDASLKAPPSTSGIIIDKQLFARAKKDRVQK
                     DQEKDQLNKLDEQHAIATNDLKTILIDKLLQLVKNKTANNVKTIYGQELFPKGTKFAL
                     ASLKELDFTTVDYGNWTDDQPTNELVARLLHNYVIKYNEELGRYKREKFNLSIGDELP
                     AGVLKLAKVYLAKKRKLRVGDKLAGRHGNKGIVSRIVREEDMPFLEDGTPVDIVLNPL
                     GVPSRMNLGQ"
BASE COUNT         2725 a         1509 c         1868 g         2253 t
ORIGIN      
        1 agagcagctg atttgtaatc agctggtcgg gggttcgagt ccctccactg gctctattta
       61 tgaaatagct gaaaggcagg taagcagaaa aacggtttac cgtacaggaa gcacaagggg
      121 gtgcgccgaa gtgggagaga cggggcagac tgtaaatctg ttggctacgc ctgaataggt
      181 tcgaatccta tcacccccac acagctacaa aagccattga aaaaactttg catgcgggtg
      241 tagctcagtt ggtagagcat cagccttcca agctgagggt cgagggttcg agtctcttca
      301 cccgctctgc ttttgcaaaa ctggctattt tgaaaaaaat aagccaatgt agctcagggg
      361 tagagcactt ccatggtaag gaaggggtcg ggggttcaat tcccttcatt ggctcaaaaa
      421 atgaaaatgt taataattat tttttaatca caaatcacaa aaaaatttaa acgatggcta
      481 aggaaacatt cgacagatcg aagcctcacc taaatattgg tactatcggt cacgttgacc
      541 acggtaagac aactcttact gcagcaatta caaacgtact tgccgaaaga ggtcttgcag
      601 aaaagaagga ttacgattca atcgacgctg ctccggaaga aaaggaaaga ggtatcacca
      661 ttaacactgc acacgtagag tatcagacag aaaagcgtca ctatgcgcac gttgactgtc
      721 ctggtcacgc agactacgtt aagaacatgg taactggtgc tgctcagatg gacggtgcta
      781 tcattgttgt tgctgcaaca gatggtccta tgccacaaac aagagaacac atccttcttg
      841 cacgtcaggt aggtgtgcct aaccttgttg tattcatgaa taaagttgac ctagttgatg
      901 accctgaaat gcttgaacta gttgaaatgg aagttagaga gcttctttct tcttatggtt
      961 ttgacggtga caatgcagct gttatcaaag gctctgcact taaagcactt gaaggtgacc
     1021 ctaaatatgt acaagctatt cttgacttga tggatgcttg tgatagtgaa atcaaagaac
     1081 caacaagaga tatcgacaag cctttcctaa tgcctataga agacgtattc tcaatcactg
     1141 gtcgtggtac agttgctacc ggtcgtattg aaagaggtgt tatcgaagtg ggtaaccctg
     1201 tagaaatcgt tggtatgatg gcagaaggtg aaaaaccgct cacatctgtt tgtacgggtg
     1261 ttgagatgtt cagaaaacta ctcaatagag gtgaagccgg tgacaacgct ggtttgctac
     1321 taagaggtat agataaagaa gctatcagaa gaggtatggt tatttgcgct cctggttcag
     1381 ttaagcctca cagaaagttc aagtgcgagg tgtatgtatt gagcaaggaa gaaggtggac
     1441 gtcacactcc attcttcaac ggataccgtc cacagttcta tttccgtaca acagacgtaa
     1501 caggtgactg taaactacct gaaggtgtag aaatggtaat gcctggtgat aacgtatcac
     1561 ttgaagtaat tctaatcaac ccgattgcaa tggagaaagg tcttcgattc gcaatcagag
     1621 aaggtggtag aacagttggt gcaggtcagg taactgagat ccttgattaa ttttaaatat
     1681 taatctcgac ctataattaa agcacgaatt aagcacctcc ttgggggtgc ttaattttcg
     1741 catatacggg catagctcaa ctggtagagt gacggtctcc aaaaccgtag gttgcgggtt
     1801 cgatccctgc tgcccgtgca aaattgaaac attaaaacaa aagctgatgg ataagataaa
     1861 attgtattta agtcagagtt atgatgaact tgtcaataaa gtttcctggc caagtacaaa
     1921 taatttaatt gagtctgcta aagtagttct gacttttgtc attatttcta ccattctggt
     1981 atttattatg gatgtcattt ctcagcaagc gcttagtttt atttatagta ttggttaaac
     2041 ttatttctaa ctgaatatta atttaccccc tgtatgtcag aagaaaagaa gtggttttca
     2101 ttgcgtgtca taagtggcaa agaaaaatct atcagagata gaattgaccg tgaaattgac
     2161 agctgtggat ggagaaatat agttacgcag gttgtagtac cttctgaaaa ggtgtacaaa
     2221 atacgtaatg gcaaaaaagt tattactgaa agaaatattc ttccggggta tattcttgtg
     2281 gaggctgatg cagaaaaatt tactgccgaa attgtgcagg gaatcgcaaa tctgcctaat
     2341 gtgattaact ttcttggtgc caataagcca ataccgatgc aaaatgcgga ggcaaataga
     2401 ttgttgggca agatggacga atcttctgag ataggagagt ctaatcttga accatttatc
     2461 gttggcgaaa ccgtaaaaat tattgacgga cctttcttcg aatttgtcgg tgatatactt
     2521 gaagtgaacg aggaaaagaa aaaattaaaa cttattgtta aaatatttgg gcgtggaact
     2581 gaggtggagc tcaatttcat gcaagtagaa aaaacgtaat attatttaaa tacatacatc
     2641 atggcaaaag aaatcgaata catcattaaa cttcaggtca aaggcggaca agcgaatcct
     2701 gcacctccaa tcggtcctgc acttggttcg aagggtgtta atatcatgga attctgcaaa
     2761 agatttaatg ctaagactca agatacacct ggtaaaatta tacctgttgt gattgaagtt
     2821 tataaggata agtcttttga gtttgttacc aaaacagcac ctgccacggt tcagttattc
     2881 gaagcagcgg gtataaagaa aggttctgca cagtctaacc gtatcaaacc ggggtctgta
     2941 acatgggatc agattaaagc catagctgaa gacaaaatgc ctgaccttaa tgcatttaaa
     3001 cttgaatctg caatgaagat gatagcgggt agtgctagaa gcataggttt ggaaataaag
     3061 ggagatgctc ctttttaaaa gaaagagttg aataaaaata ttttttaact gaagggagtt
     3121 ctgttgttaa tagcagaatg tctgaacctt aaattttatt tatggcaaaa ttatctaaaa
     3181 aaagagcagc aattgctgca aaagttgacc aagataaatt gtacacattg cccgaggcaa
     3241 tgaatttggt caaagaagtg aacactacaa aatttgacgc ttctgttgat gtacacatca
     3301 gattaggggt ggacccaagg aaagcagacc aggcacttcg tggtactgta accctacctc
     3361 acggaacagg aaaaacaaaa agagtacttg tactttgtac gcctgacaag gcagctgaag
     3421 caactgcagc aggtgctgac cacgtaggtc ttgaagatta cattcaaaag atagcaggag
     3481 gctggactga tgttgacgtt atcgtagcaa tgcctacagt aatggctcag ctcggtaaaa
     3541 tcggtagaat actaggccct cgtggtttga tgccaaaccc taaaacaggt acagtgacta
     3601 tggatgtcgc ctctgcagtt caggacgtaa aaggtggtaa aatttctttc agagttgata
     3661 aatatggtat cgtacactct tctgttggta gagtatcatt ttcacctgaa aagttagaag
     3721 gaaatgctaa tgaacttata agtacattgg tgaaaatgaa accatcatct gccaaaggta
     3781 cttatcttaa gtcgatatca gtggcaggta ccatgagtcc gggtataaaa gttgacccta
     3841 aaactattaa gtaatcaaca tctaaagcta tcaaattatg aataaatcaa ataaaggagt
     3901 agcaatcgaa gaattgaagg agaagctgga aacagcgaat tttttctata tcaccgattc
     3961 ttcttcactt tccgtagaaa aaataaataa tcttagaaga atctgtttca acaaaggtgt
     4021 tgaaatgaaa gttgttaaaa acactttgtt gagaaaggcc atggaatctt ttggagaaga
     4081 aagaggatac cagggtctgt ttgatgcttt gcacggtcct acggctatca tgtttacaga
     4141 aacggccaat gttcctgcaa agattatcaa agagtttaga aaagatgata aaagaccaat
     4201 tattaaggct gcatatatag acactgcaat atttatgggc gaccaatcta ttgaagatct
     4261 agtgaacctt aagtcaaaag agcaattaat cggggaagtt attacaatac ttcagtctcc
     4321 tgccaagaat gttatcagtg cgcttaagtc aggaggacag acaattgccg gattggttaa
     4381 ggcactggaa gaaagagctg aacagtaatt tcttcacacg gatatggctt ccaagtgata
     4441 ccacgcactt aattttatag taactttaaa aactaaaaaa aatggctgat ttaaaagcac
     4501 ttgctgacca acttgtaaat ttaactgtta aagaagttaa tgagttggca actattctaa
     4561 aagatgagta tggtatcgaa cctgctgctg ctgctgttgc agttgctgct gcccctgcgg
     4621 gtggtggagc tccagaagct gctgctgaaa aaacatcatt tgatgtattt atgacttcag
     4681 ctggtgcggg taaattgaat gttgtaaaag aagttaaaaa ccttcttggt cttggtttga
     4741 aagaagctaa agatcttgtt gacgccgctc ctgcaatgat taaggaagga tgttcaaagg
     4801 atgaagctga atcacttaag gctgctctag aaggcgctgg tgcatctatc gaattgaagt
     4861 aattctgaac gtatagacaa acctatgctt atgaagagcc tctgtggcat tcaaaatgta
     4921 catacagtaa tataggttta agtaaatggt tagctccatt tatttaaacc tattgctgtt
     4981 tgttttaaag agaatattca aaatctatta tatgcaaaac taattcgctt tagttttgtt
     5041 aaaatttaaa taatctggat aatggctaac aacagcgcaa tgctctatgc cgaaagcaaa
     5101 agagtaaatt tcggaaaaat caaatatcct cttacacctc ctgatctatt agatatacag
     5161 ctaaaatcgt tccaagaatt ttttcagctc gaaacaactc cggaaaacag atcggatgaa
     5221 ggcttgttca aagtgttcca ggaaaatttc ccgattactg atgccagaaa tatatttgtt
     5281 ctggaatttc aggattattt tatcgatcca cctcgttatt cgatagacga atgtattgaa
     5341 agagggctaa cttattctgt tccactcaaa gcaaagttgc gtttgtcttg taacgacgaa
     5401 gaacacgttg actttgaaac tatagtacag gatgtgttcc tcggcaatat accgtacatg
     5461 acacctaaag gtacatttgt tatcaatggt gcagaaaggg ttattgtttc ccaacttcac
     5521 agatcgcctg gtgtattctt cggacaaagt gtccacccga atggtagcaa aatttattcc
     5581 gcaagggtaa tcccttttaa gggtgcatgg atggagttcg ctaccgatat caacacggtg
     5641 atgcatgctt atatagaccg caaaaagaaa ttcccggtaa caacgctact tcgtgctatt
     5701 ggctttgata cagacaaatc aatcttgaac atttttggcc ttgctgatga agtaccatct
     5761 acacgcgaag cacttgaagc ttcaataggc cgaaaactgg ctgcccgtgt gttacgaaca
     5821 tggactgaag attttgtgga tgaagataca ggtgaggttg ttacaataga aagaaatgac
     5881 attatcctag agcgagatgt ggttattgaa gcggataata ttgatttgat tctgaatgcg
     5941 gggctcgaat cagtcattct tcaaaaagaa aatataggag aagattattc cattattttc
     6001 aatacactcc agaaagaccc taccagctcc gaacttgaag cggtacagta tatttatcgt
     6061 cagctgagag gtacagaacc accggatgat gaaacagcaa ggggaatcat cgataaactg
     6121 tttttctcag ataagagata caaccttggt gaagtaggtc gttacaaaat taataaaaag
     6181 ctcggtattg atacttctga cgagacactc gtgcttacaa aggaagatat tatccttatc
     6241 gttaaatatt tggtcagcct cattaaccaa agggcagaaa tagatgatat tgaccacctt
     6301 tcgaacagaa gagtacgtac ggttggtgaa caattgtatg cacagtttgg tgtaggtctt
     6361 gcacgtatgt caagaaccat tcgcgaaagg atgaacgtaa gggacaatga agtattctca
     6421 ccggttgatt tgataaatgc gagaacattg tcgtcagtta tcaattcgtt ttttggtaca
     6481 agccagttgt cacaattcct tgaccagacc aatccactat ctgaaatcac tcacaaaaga
     6541 aggatttctg cactaggtcc gggaggtttg tcgagagaaa gagcgggctt tgaggtgagg
     6601 gacgttcact attcacacta cggacgactt tgtacaatcg aaactccgga aggtccaaac
     6661 atcggtctta tatcaaccct gtgcgtacat gccaagatta acaaaatggg attccttgag
     6721 actccataca gacctgttaa caatggtgtt gtcgaattaa aatctgaagc catttatttg
     6781 tcggcagaag gagaagatta ttcaaaaatt gcacaggcaa atgcgccatt gacagatgtt
     6841 ggaaactttg aaagtgataa ggtaaaatgt cgggaacatg gagaatttcc ggttttaggt
     6901 ccggatgaga tagaatatat ggatgtggca cctaatcaaa tagttggtgt ttctgcatcg
     6961 ctcattcctt tccttgaaaa cgatgacgcc aaccgtgcac taatgggctc taacatgcaa
     7021 cgccaggcag taccactcat caggcctact tctccaatag tgggcacagg tcttgaaggt
     7081 aaagtggcaa gagactcaag gatgttgata aatgctgaag gtgatggtta tgtagaatat
     7141 gttgatgcag aagaaatcgt catccgttat gacagaaccg atgaagacca gattacttcc
     7201 ttcgaagata acagaaagac ttacaaatta accaaatttg ccagaaccaa ccagggtacg
     7261 tgtatcaacc tgaagcctat tgtcactaaa ggccaaagag tagctaaggg tgaaatcctc
     7321 tgtgatggtt atgctacgga caaaggtgaa cttgcactcg gacaaaacct aaaggtggct
     7381 ttcatgcctt ggaaaggtta caacttcgag gatgctattg tattatctga aagggttgta
     7441 aaggaagaca tttttagctc aatccatatc gaacacttcg aaatggatgt acgcgatact
     7501 aaacttggtg aagaagaact taccaatgat attccaaatg taagtgaaga agcaacaaag
     7561 gacttggatg aaaacggtat catcagagtt ggggcatggg ttaaagatgg tgatatactc
     7621 attggtaaaa ttacacctaa aggtgagtct gacccaacac cggaggaaaa gctgctaaga
     7681 gcgatattcg gagacaaggc aggtgacgtc aaagacgcat ccctcaaggc acctccttct
     7741 acatcaggta taatcattga caaacagttg tttgcaaggg ctaagaaaga cagagttcag
     7801 aaagatcagg aaaaggatca gcttaacaaa cttgacgagc aacacgccat tgctaccaac
     7861 gacttgaaga ccattctaat cgataaactt ctgcagttgg ttaaaaataa aactgccaac
     7921 aatgtaaaaa ccatctatgg acaggagttg ttccctaagg gtaccaaatt tgcacttgct
     7981 tcactcaaag agcttgactt caccactgtg gactatggca attggacaga tgatcagcca
     8041 acaaacgaac tggtagcaag attgctccat aattatgtta tcaagtacaa tgaagaactt
     8101 ggccgctata agagggagaa attcaacctc agcattggtg atgagcttcc tgcaggcgta
     8161 ctcaagctgg caaaagtgta tcttgcaaaa aaacgtaagc taagagtagg tgataaactt
     8221 gccggccgac acggcaacaa aggtattgtg tcaagaatag tacgagaaga agatatgcca
     8281 ttccttgaag atggcacacc ggtagatatt gtactgaatc ctctgggtgt accttcaagg
     8341 atgaacctcg gtcag
//
LOCUS       DAOQQY010000101         8326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3338_length_8326_cov_6.787087, whole genome shotgun sequence.
ACCESSION   DAOQQY010000101 DAOQQY010000000
VERSION     DAOQQY010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3338_length_8326_cov_6.787087"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLE23_06300"
     CDS             complement(<1..183)
                     /locus_tag="PLE23_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX82908.1"
                     /translation="MKYNVIVIGGGVVGLASAYRILVQQPGTKLCILEKEAAIALHQT
                     GNNSGVIHSGIYYKPGS"
     gene            complement(351..3317)
                     /locus_tag="PLE23_06305"
     CDS             complement(351..3317)
                     /locus_tag="PLE23_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMX82909.1"
                     /translation="MLKKIYLIAIGLLIFSVGAIAQNTLRPTTKTSADGKYQWVEVEG
                     DLTKSRFYTLNNGLTVILSENHLEPRIMSLITTKAGSKNDPHDHTGLAHYLEHMLFKG
                     TDRFGTLDYEKEKIQLDRIDALYDQYNKQTDAAVRKAIYHQIDSVSGEAAKYAIANEY
                     DKMMSSLGSNMTNAFTSFENTTYMENIPSNNLDKFLAVQAERFRHPVLRLFHTELEAV
                     YEEKNISLDDGNSKVYEAMFASLFKNHTYGTQTTIGTIEHLKNPSLTEIRKYFYNYYV
                     PNNMAIILTGDFNSDEVIRQVDRHFGQWQPKQVPEFTFTPEKPRTQIERIDVTSPDEE
                     GVAIGFIMPNTNDKESVIANLVSNILYNGKTGLIDKNLVLQQKVLEAFGFNESLKDYG
                     IFYLGGRPLDGQSLKQVEELIIAQINDLKNGKFDEELITGIINNEKVSKIKERESPMN
                     MAFILNDIFVTGKPWEKYLQSVDDMSKITKQDVVNFAKKWFGDNYTVVYKHTGIDKNV
                     IKVDKPEITPVPVNRESQSAFLKQIIESPSKPIAPVFLDYDKDIQKTELKKDLPLWYV
                     KNELNDLFSAYYVLDMGKENMAKLPLAVEYLKYIGTSKMTNEQFNKELFKLAVDFSVF
                     AGDDQVYISFSGLNENFEKAIKLVETLLNDPKANQEALNKLIDATIKSRNDATLNKGA
                     IFWGGLENYADYGSKNPFNDVISNADLKKIKAEELTAIIKSLTSYKHKVFYYGPKPFN
                     SLTSSLTANHKTPKILKNYPAAKVYPKVQPQENTIYFVNYDMVQAEIGMNRWGQAFDQ
                     NLLPKVRAFNEYYGGGMGSVVFQDIRESKALAYSSYASYGTPSKKEDPFSATFYVGTQ
                     SDKLHQAITAMNDLLQNMPESEKLWEISKQSIKQGIETKRITKTGILFNYQNALKLGM
                     NTDSRKLIYNQIDQISLADIKKFEEENLKNKKWNIKLIGSKDKIKLEELKKYGKVVEL
                     SMKDIFGYDVESTVKP"
     gene            complement(3401..4501)
                     /locus_tag="PLE23_06310"
     CDS             complement(3401..4501)
                     /locus_tag="PLE23_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMX82910.1"
                     /translation="MSHEFHVLTIGNISKETPDTVTLHLNVPESLEEKFRFRSGQFLT
                     LRFMLKGKEERRSYSICTAAFENKLSITVKRVMGGLVSTYIVENLKAGDTIEVMAPEG
                     KFALNSDYNIGKSYYFVAAGSGITPVISHIKTLLEEEPQSSLFLLYGNRNEQSIIFRQ
                     QLDSLQKYYSGQLHIEYVLSQPKREKPSGLGGFFSKGTISWQGKTGRINAKMLEEFMS
                     EYPPRHKVSEYVLCGPGQMIDDVTSYIKHLGIDKKHIHAEYFTTDNTPDHISDKSSVE
                     ATLVAKLNGNVTETSLPAGKTILDKLIDMKKDPPYSCRAGSCSTCMAKVISGTVEMKV
                     CHALTDEEIEEGYILTCQSLATSSSVEVDYDV"
     gene            4757..5473
                     /locus_tag="PLE23_06315"
     CDS             4757..5473
                     /locus_tag="PLE23_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX82911.1"
                     /translation="MPQKLSIVIPAYNEEKTIHHILDKVKAVVLIENIEKEILIINDC
                     SKDDTEGAIQRYMTQNPGIPIQYFKHDVNRGKGAALHTGIEKATGDYIIIQDADLEYD
                     PEEYNILLKPILSGFADIVYGSRFMGGKPHRILFFWHSIGNKFLTFLSNMFTNLNLTD
                     METCYKLFKADIVKNIKLNENRFGFEPEVTSKIAKLKGIRIYEVGISYYGRSYEEGKK
                     IGWKDGFRAIYCILKYGLFG"
     gene            complement(5477..6298)
                     /locus_tag="PLE23_06320"
     CDS             complement(5477..6298)
                     /locus_tag="PLE23_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HMX82912.1"
                     /translation="MKNFLVIILLLAGSGYLSAQRGWELGGMAGVSNYFGDLNSNYGL
                     NHIGPSARLFARYNFNTRLCLKFAASYARVAGYDSDSDNTFEKQRNLSFASNIFDGAA
                     EFEFNFMPYVHGSAYNNFTPYGFLGLAAFKFNPKTKYEDKWYELQPLGTEGQFLGQEY
                     SLLQLGFAYGLGLKWDINYDWSINVELASRYLFTDYLDDVSTIYPDAKDVQRLHGDIA
                     AALVDRSGELGIEPPIGQKGRQRGNSKDNDSYQMLTVGLSYYFGTIRCPSISNYY"
     gene            complement(6367..7647)
                     /gene="serS"
                     /locus_tag="PLE23_06325"
     CDS             complement(6367..7647)
                     /gene="serS"
                     /locus_tag="PLE23_06325"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMX82913.1"
                     /translation="MLQVSVLRENKEKVIQGLKKRNFSDQDLVIIDTIIVLDDERKLI
                     QKSLDDKLNISNQVAAKIGQLIKNGEKGAAEAAKSEVASLKKDIQQEEEKMRTVSTQL
                     ETHLLNLPNVPHDSVPPGKSADDNFVFQDWPQALPELPDTALPHWDLAKKYDIFDLEL
                     GTKITGAGFPVYRKKGARLQRALINFFLDQATNEGFEEIIPPLMVNEASARGTGQIPD
                     KEAQMYYIERDDLYMIPTAEVPVTNIYRDVILNEDQFPVKMTGYTPCFRREAGSYGAH
                     VRGLNRVHQFDKVEIVVLEHPDRSYQMLQQMIAHVSGLMEKLELPYRILHLCGGDMGF
                     ASASTYDFEVYSAAQKRWLEVSSVSNFESFQANRLKLRFRDGSKKTQLAHTLNGSALA
                     LARIVAALLENNQTPEGIRIPDILVPYTGFKILN"
     gene            7937..>8326
                     /locus_tag="PLE23_06330"
     CDS             7937..>8326
                     /locus_tag="PLE23_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HMX82914.1"
                     /translation="MYDILQLNEMLMPELRDIAEKLNIENFKRLAKQDIIYKILDEQA
                     LSQNPKTATSSKKSEPVVVEAKQQSTKPKSVAGHKNKNDNPPQVKPAMSAQEENAISS
                     EESELKTKRTRKITTKPAADKVIPATKE"
BASE COUNT         2279 a         1621 c         1706 g         2720 t
ORIGIN      
        1 actaccgggc ttgtaatata taccgctgtg tatgacacca ctgttatttc cggtctgatg
       61 tagtgctatt gccgcttctt tttccagtat gcacagtttg gtacccggct gttgtacgag
      121 aatgcggtag gcggatgcaa gacccaccac acctcctcct attactatta cattgtattt
      181 catttctctg acattaaata ccaaagatat aaaatgactg cctgaatcga gtataaacag
      241 cagatttcaa cacagaaaaa aaatcccgaa acttgtaagc cagaggtttc gggaaatgta
      301 tttatatttt aaaccatgaa aactatcaat aaatcagttg cctgagatag ttaaggtttg
      361 acggttgatt ctacatcata gccaaagatg tctttcatac tcaactctac tacttttccg
      421 tattttttca attcttcgag cttgatttta tctttagagc caatgagctt gatgttccat
      481 tttttgtttt tcaggttttc ttcttcaaat tttttaatat cagccaggct tatttgatct
      541 atctggttgt agatgagctt tcttgagtcg gtgttcatgc ctagtttgag ggcattctgg
      601 taattgaaca gaatacctgt tttggtaata cgtttggtct ctattccttg tttgatagat
      661 tgtttggata tctcccacaa tttctcagat tcgggcatgt tttgcagtaa gtcgttcata
      721 gctgtgatgg cttgatgaag cttgtcagat tgtgtaccta cataaaatgt ggcactgaac
      781 ggatcttctt tttttgaagg tgtgccatag ctggcgtatg aagagtaagc aagggccttt
      841 gactcgcgga tgtcctgaaa cacaacggag cccataccac caccataata ttcgttgaat
      901 gcccttacct tcggtagcag gttttggtca aatgcctggc cccatctatt catgcctatt
      961 tctgcctgta ccatatcgta attaacgaag taaatggtat tttcctgcgg ttgtactttt
     1021 ggatacacct ttgccgccgg atagttttta agtatttttg gtgttttgtg gttggcagta
     1081 aggctactag taaggctgtt aaatggtttt ggaccataat aaaatacctt atgcttatag
     1141 cttgtcaggc tttttatgat agcagtaagt tcttcggctt ttattttttt cagatcagca
     1201 ttcgaaatga catcattgaa aggattttta cttccgtagt ctgcataatt ttcaagtccg
     1261 ccccagaaaa tggctccttt gttaagtgtt gcatcatttc tgcttttgat ggtggcatct
     1321 atgagtttgt tgagtgcttc ctgattggct tttgggtcgt tgagtagagt ttctacgagt
     1381 tttattgctt tttcaaaatt ttcattaagc ccgctgaagc ttatatatac ctgatcgtct
     1441 cctgcaaata cactaaagtc aacagcgagt ttgaacaatt ctttgttgaa ctgttcgttt
     1501 gtcatttttg atgtaccgat gtatttcaga tattcgacag caaggggaag tttcgccata
     1561 ttttccttgc ccatatcaag cacataataa gcgctgaaca aatcattcaa ctcgtttttt
     1621 acgtaccata gtggcaaatc tttctttagt tccgttttct gaatgtcttt gtcataatca
     1681 aggaataccg gggcaattgg tttggatggt gattcgataa tctgtttcag gaaggccgac
     1741 tgcgactcac ggttgacagg taccggtgtt atttcaggtt tatcgacctt gattacattt
     1801 ttgtcgatac cggtatgttt ataaacaaca gtataattat caccaaacca tttttttgca
     1861 aaattgacga catcctgttt tgttatctta ctcatgtcgt caacgctttg tagatacttt
     1921 tcccatggct tgccggttac gaagatgtca ttgagtatga atgccatatt catcgggctt
     1981 tcgcgttctt ttatctttga tactttttcg ttgtttataa tgccggttat gagttcttcg
     2041 tcaaattttc cgtttttaag gtcatttatc tgtgctatta tcaattcttc aacctgcttt
     2101 aggctttgtc cgtcgagtgg tcttccgcca agataaaata taccatagtc cttgagtgat
     2161 tcgttgaaac caaatgcttc aagtactttt tgttgaagta ccaggttttt gtcgatgagt
     2221 ccggttttgc cattgtacaa tatattactt accaaatttg caataacaga ttccttgtca
     2281 tttgtattgg gcatgataaa tcctatagct actccttctt catccgggct tgtcacatcg
     2341 attctttcta tctgggtacg tggtttttcg ggtgtaaatg taaattcggg aacttgtttt
     2401 ggctgccatt gaccgaagtg tctgtccact tgtcgtatta cttcatccga gttgaagtcg
     2461 cctgtgagaa taatggccat attattcggc acataataat tgtaaaaata tttccggatc
     2521 tctgtaagcg aagggttttt gaggtgttcg atggtaccga tggtggtctg tgtgccatag
     2581 gtatggtttt tgaataggct ggcaaacatt gcctcgtaca cttttgagtt gccatcatca
     2641 agactaatat ttttctcttc atacacagcc tctagctcgg tatggaacaa tctaagcaca
     2701 ggatgtctga atctttccgc ttgtacagca agaaatttgt caagattgtt cgaaggaata
     2761 ttctccatgt atgtagtatt ctcgaatgaa gtaaaagcat tggtcatatt ggagccaagg
     2821 cttgacatca ttttgtcgta ttcgtttgca atggcatatt tggctgcttc accggacacc
     2881 gagtctatct ggtgatagat ggccttacgt actgctgcat ccgtctgctt attatactgg
     2941 tcataaagag cgtctatacg gtccagttgt atcttttctt tttcataatc aagtgtgccg
     3001 aagcggtcag tacctttgaa cagcatgtgt tcgagatagt gtgcaagtcc ggtgtggtcg
     3061 tgagggtcat ttttactgcc tgctttggta gtaataagtg acatgatcct tggttcgaga
     3121 tgattttctg aaagtatgac ggtaaggccg ttattgagtg tataaaaacg tgacttggtc
     3181 aggtctcctt ctacctcgac ccattggtat ttgccgtcag cagatgtctt ggtagtcgga
     3241 cgaagtgtgt tttgggcgat agctcctaca gaaaatatca ataatccgat ggcgatcagg
     3301 tatatttttt taagcatttg gtgaaaaatt ttattaaaaa taatgtaaga aagctttaca
     3361 aaagtatgaa aattaatgag atgctttatg aaaatttttc ttaaacatca taatctacct
     3421 ctacgcttga tgatgtggca agagattgac aggtaagtat atagccttct tcaatctcct
     3481 catcggtgag ggcatggcat accttcattt ctactgtacc gctgatgacc ttagccatac
     3541 atgtagaaca cgaaccagcc ctacaagaat acggcggatc tttcttcata tcaatcaact
     3601 tatcgagaat ggttttaccg gcaggcagac tggtttcggt tacatttccg tttagtttgg
     3661 ctacaagcgt tgcttctaca gatgacttgt cagaaatatg gtcaggagta ttatctgtgg
     3721 tgaagtactc tgcgtgaatg tgttttttgt caatgcctag atgtttaata taggatgtga
     3781 cgtcatctat catctgaccg ggcccgcaaa gcacatattc cgacacttta tggcgtggtg
     3841 ggtattcact cataaattct tcgagcattt tggcatttat tctaccggtt ttaccttgcc
     3901 atgatatggt ccctttagag aaaaatcctc caagtccgga tggtttttca cgttttggtt
     3961 ggcttagtac atactcaatg tgtagctgcc cactatagta cttctgtagc gaatcgagtt
     4021 gttgtctgaa aatgatggat tgttcatttc tgttgccata gagcagaaac aggctgcttt
     4081 gtggctcttc ttccagaagc gttttgatat gcgatattac cggagtgata ccactgccgg
     4141 ctgctacgaa ataatagctt ttgccaatat tataatcgga gttcagtgca aatttgcctt
     4201 caggtgccat cacttcaatc gtgtctcctg cttttaggtt ttcaacaata tatgtgctta
     4261 ccaggccgcc cattactctc tttacggtta tgcttaattt gttttcgaat gcggctgtac
     4321 agatggaata cgacctgcgt tcttccttgc ctttaagcat aaaccgtagg gtaagaaact
     4381 gcccgctgcg aaatctgaat ttttcttcaa gtgactcagg tacgttaaga tggagtgtta
     4441 cagtatcagg cgtctcctta gaaatattgc caatggtaag tacatgaaat tcgtggctca
     4501 taaatcgaaa ttaatgatgt gttttttgat acctgagtac aggcattttg ttaaatgagg
     4561 gagcaaagtt aggtatattc gggctggtcg gataatatta ttccccaaaa actatgataa
     4621 aatagtttca gattaattat taaggataaa tctcatattt ttgcagtcgg ccatttagcc
     4681 cgaacgtttg ttttaaaatt tttttcaccc taaaaaatac aatcatagcc acgcaaaagg
     4741 ctagaatcta atagttatgc cacaaaaact ttcaatagtc attcctgcat ataatgaaga
     4801 aaaaaccatc caccacatat tggataaagt gaaggctgtg gtattaattg agaatatcga
     4861 aaaagagatt ttgatcatca atgattgttc taaagatgat accgaaggcg ccattcaaag
     4921 gtatatgaca caaaaccccg gcataccgat acagtatttc aaacatgatg ttaatcgtgg
     4981 aaaaggcgct gctctgcata ccggcataga aaaagctacc ggagactaca tcattataca
     5041 ggatgccgac ttagagtatg atccggaaga atacaatatt ctgctcaaac ctattctgag
     5101 tggatttgcc gacattgttt acggatcgag gtttatgggt ggtaagcctc atcggattct
     5161 gtttttctgg cactcgattg gcaataaatt tctgactttc ctgtctaata tgtttaccaa
     5221 cctcaacctg acagacatgg aaacctgcta caaactcttc aaggcagata ttgttaaaaa
     5281 tataaaactc aatgaaaacc gatttggttt tgaacccgaa gtaacttcca aaatagccaa
     5341 attaaaaggt atcaggatat atgaagtagg tatttcttat tatggacgtt cgtatgaaga
     5401 aggcaagaaa ataggatgga aagatggttt tcgtgccata tactgcatac tcaaatacgg
     5461 tttgtttggt taaaacttaa tagtaatttg aaatagacgg acagcggatg gtaccgaaat
     5521 agtaggaaag acctaccgta agcatctgat agctgtcgtt gtccttactg ttgccgcgtt
     5581 gtcttccctt ttgcccgatg ggtggttcta tgcccagttc gccagacctg tcaactaggg
     5641 ccgctgcaat atcgccatgc agacgctgaa catcttttgc atcgggataa atggtactga
     5701 catcatcaag atagtctgta aatagataac gagacgccag ctcaacattg atgctccagt
     5761 cgtagttgat gtcccattta aggcccagac cgtatgcaaa acccaattgg agtaatgaat
     5821 attcctgacc gaggaactga ccttcggtac caagcggctg tagctcgtac catttatctt
     5881 catattttgt ttttgggtta aatttgaagg cggcaagccc tagaaagccg taaggtgtaa
     5941 aattattgta tgcgctacca tgcacataag gcatgaagtt aaattcgaat tctgctgccc
     6001 catcaaagat gttgcttgca aagctcaaat tgcgctgttt ttcgaatgtg ttgtcagaat
     6061 cggagtcata accagctaca cgagcatagg atgccgcaaa tttcaggcaa agtcttgtgt
     6121 tgaaattata tctggcaaac aacctggcag aaggaccaat gtggttgaga ccgtaatttg
     6181 agtttaggtc gccaaaataa ttgcttacac ctgccattcc ccctagttcc cagccccgct
     6241 gggcagaaag atagccggag cctgctaata ataagatgat tacaaggaaa ttcttcaatg
     6301 tgataaggtt gtttaatgct tataaacgtt tttacaatat tttattgttt acgtcatcca
     6361 ttgaatttag ttcagaattt tgaatccggt ataaggcaca aggatatctg gtatgcgtat
     6421 gccttcaggt gtctggttgt tttcaagcag ggcggctact atccttgcta gtgcgagtgc
     6481 actaccattg agagtatgtg ccaactgggt tttcttcgaa ccatctctga agcgcagttt
     6541 gagtcgattg gcctgaaagc tttcaaaatt tgaaacagaa ctgacctcaa gccatctttt
     6601 ctgtgcagct gaatacactt cgaagtcgta ggtggatgct gatgcaaaac ccatgtcgcc
     6661 accgcacaag tgcagtatcc tgtaaggcag ttcgagtttt tccataaggc cagatacgtg
     6721 ggcaatcatc tgttgtagca tctggtacga cctgtctgga tgctctaaga ctacaatctc
     6781 aaccttatcg aactgatgaa cacggttaag gccacgtaca tgcgccccgt acgaacccgc
     6841 ctcacgtcta aagcaaggag tatatccggt catttttaca ggaaattggt cttcattgag
     6901 gatgacatct ctataaatat tggtgactgg tacttcggca gtcggtatca tgtagaggtc
     6961 atcacgttct atgtagtaca tctgggcttc tttgtccgga atctgacctg taccgcgtgc
     7021 cgatgcttca tttaccatta atgggggaat gatttcttca aaaccctcat tagtggcttg
     7081 gtcgaggaaa aaatttatta gagcacgttg tagtcttgct cctttttttc tgtaaacagg
     7141 aaagccggca cctgttatct tagtgccgag ctccaggtca aatatatcat actttttggc
     7201 aagatcccag tgaggtaggg cagtatcggg caactccggc aatgcttgcg gccagtcttg
     7261 gaagacaaaa ttatcatcag cacttttgcc cggaggtacc gaatcgtgcg gtacattggg
     7321 cagattgagc agatgtgttt cgagttgtgt gcttacagta cgcatctttt cctcttcttg
     7381 ctgaatatct tttttcagac ttgctacttc cgatttcgct gcttctgcag cacccttttc
     7441 accattcttg atgagctggc ctatttttgc agctacctga tttgagatgt ttagtttgtc
     7501 atcgagtgat ttttgaatca atttgcgttc gtcatcaaga acgatgatgg tatcaattat
     7561 tacaagatcc tgatcagaga aatttctttt cttcaaaccc tggatcactt tttctttatt
     7621 ttctcttaaa acactgactt gtaacatatt aatgaattag tattagatgt aaaaatatat
     7681 ttaaccatta aggtagaaca aaaaaaatgt gaattcgtat aaaattttgt acgctcaaag
     7741 atactattag attcaaaata ttttgtaatt ttgtggcgtg ctgtttgaag tcttccttta
     7801 agcattttca tttcacacta ttttatcatt attcaaaccg ttttcggaaa caatatttga
     7861 ctatataata ttatagtgtg ttcaaaaaac attctttcaa tttctcaacc ttacaattct
     7921 taattttata ccttttatgt acgacatact tcagttaaat gagatgctga tgcctgaatt
     7981 aagggacata gctgaaaaac ttaacattga aaattttaag agattagcta agcaggatat
     8041 catatataag atacttgacg aacaggctct ttctcagaat cccaaaacag caacaagctc
     8101 caaaaaatcg gaaccggttg ttgttgaagc aaagcaacag agtactaaac ctaagagtgt
     8161 agccgggcac aaaaacaaaa atgataaccc accacaggtc aagcctgcaa tgagcgcaca
     8221 agaagaaaat gcgatatcaa gtgaagagtc ggaactaaaa accaaaagga caaggaaaat
     8281 aactaccaaa cctgccgccg ataaagttat acctgccacc aaagaa
//
LOCUS       DAOQQY010000102         8270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3374_length_8270_cov_6.006452, whole genome shotgun sequence.
ACCESSION   DAOQQY010000102 DAOQQY010000000
VERSION     DAOQQY010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3374_length_8270_cov_6.006452"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="PLE23_06335"
     CDS             complement(<1..436)
                     /locus_tag="PLE23_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HMX82915.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGKEPVVIPNDEGRRTTPSIVAFMD
                     NGERKVGDPAKRQAITNPTKTIYSIKRFMGARYSESTAEAAKMPYKVVKGDNDTVRIQ
                     IDDRGYSPQEISAITLQKMKKVAEDYLGQEVTEAVVTVPAYFN"
     gene            complement(591..1601)
                     /locus_tag="PLE23_06340"
     CDS             complement(591..1601)
                     /locus_tag="PLE23_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82916.1"
                     /translation="MKNRIILLLAAIVAFSSCDNELHLVAPWKRIPVVYGFLNLSENA
                     QYIRVEKAFLDPEKSAEQIAKNPDSLYFNNIEVSLTRVKNGTTWVLTRVNGTDEGFPK
                     KPGTFAQDPNYLYKINTKDIELVAGEQYKLSIKDLSNNTTIAEATTNVVGNYNMVATD
                     PPNPGAWPYKSDVRFSWRSNETVGKVYDVFMKFYYNENTIDQPSKFAKKSLTWKIAEN
                     VQRPADPTSRITAMLKGEEIFKFLGENISKIPGIKRIFLSYDVTVVAGGQEFQDYIDL
                     GNASSGITSSQILPTYSNISNDGIGIFSSRNILEAVDYSINTPTRDSLKSGIYTKDLG
                     FQ"
     gene            1739..2554
                     /gene="panB"
                     /locus_tag="PLE23_06345"
     CDS             1739..2554
                     /gene="panB"
                     /locus_tag="PLE23_06345"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMX82917.1"
                     /translation="MSVEKQIKRVTVHTLQAMKENHEKISMLTAYDYSMAKVLDESGI
                     DILLVGDSASNVIAGHETTLPITLDQMIYHAASVVRGVERALVIVDLPFGSYQGNSEK
                     ALDSTIRIMKESGAHGVKLEGGIEIEESVRRILSAGVPVMAHLGLTPQSIYKFGTYTV
                     RAKEEAEAKKLMEDALLMQEIGCFAVLMEKIPAELATKVTQALHVPTIGIGAGGGVDG
                     QVLVTHDLLGLTKEFKPRFLRRYMDLFVDIKAAVQHYITDVKNADFPNENEQY"
     gene            2555..3643
                     /gene="mltG"
                     /locus_tag="PLE23_06350"
     CDS             2555..3643
                     /gene="mltG"
                     /locus_tag="PLE23_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMX82918.1"
                     /translation="MKLNFEGAQMWFRKAKIWLLNPANRKPVYIIGGVLATGLIVFIW
                     LLATLFLSNVKIEEAKAFYIYDNYTLDSIANKLHSEQLISNEGSFRTAAGLLGLNVKN
                     IRVGRYKIEPGSGNFTLVRMFKNGLQTPVKITLNNIRTIEELAGKLGSYLKSDSTAFM
                     HLFRDTTFLSKHYLDSETVMTAFLPDTYEMYWDTKPDEVFEKFANVTVKFWNKKRLTK
                     ADSLQLTPQQVYTLASIVEKETNKVDERPDVAGVYLNRFHQNMPLQADPTVVFANNDF
                     TIKRVTGKHTAIDSPYNTYKYTGLPPGPICMPEKSSIDAVLNATNHDYMFFCAKPDLS
                     GYHSFAVTFQQHVQNAKAYRDFLDKNGY"
     gene            3643..4980
                     /gene="tilS"
                     /locus_tag="PLE23_06355"
     CDS             3643..4980
                     /gene="tilS"
                     /locus_tag="PLE23_06355"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HMX82919.1"
                     /translation="MMWMMPTERHFLKAMEGFFPDGVSDKRFLLAVSGGADSMVVLHL
                     FQKHYSDFIIAHCNYGLRGTESDLDEALVNKMAKEYDKIFCVKHVSLPQKDNKSIQLL
                     AREHRYDWFAMLKDEHQVDFVVTAHHLDDNTETLLFNLIRGTGIKGLCGIPAMSANII
                     RPLLQVSKSNILEYAQANGIQYRDDASNFSTKYNRNKIRLNILPEIEKINPQFSKTIN
                     EHIREYADLEELLREQTANFIKHHCRKDNDCLVIPSKTLLCKPGRLNILFSILGEYGF
                     SKSQVEFLLNGQLTRSGTKIESGSHELYLHGDEWIVAPVLSDFAEISVSQIPATVHTA
                     FGSLSFIVATESTTKTERDTLVFEHNFDDKALVIRTRKDGDRFAPEGMNGKSQKLKDF
                     FINSKISVPAKARQLLLCSGDEIIWIVGLRKSHHTHKKVNNGTFVHVVFSKIT"
     gene            5178..6011
                     /locus_tag="PLE23_06360"
     CDS             5178..6011
                     /locus_tag="PLE23_06360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82920.1"
                     /translation="MKKQILILFILLHATLGNGQNLISNGRFQYPDLNPRCDGWFNLC
                     GEELTAHCDTNLNCYVGLVIDSPSWIPEDIWSLQVKAAFEPDGYAETYITGQTGTNIY
                     TLTYWMNAKDWAGGARIGTGKQNKFVESQSRSDTCSSWSEFTLTDTITTQATDTITVR
                     LSAGSGDFCICPPIRFAFIELVKKAINAVDDVNPVQSSILAYPNPAQKEIKFEVKSKN
                     QGSMTLEIFNSSGTPILSKQCTDGLFLVNRSELPGGLYIYCVTSDSDQIIVGRGKFVF
                     D"
     gene            6198..6662
                     /locus_tag="PLE23_06365"
     CDS             6198..6662
                     /locus_tag="PLE23_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82921.1"
                     /translation="MKSKLLFLGLTIILLSSCCKEKNYLGSGEYEYFAFGHFFGECGG
                     ENCVVRYVITGNQLLEDTDDSDKTEKFVKLDQELYYQVKDLEDKIPQEMFDSPQNVYG
                     CPDCADGGGLLIEYRKNGKTRKWALDYTKHELPVQIGKFVDAIHEKIYLINK"
     gene            complement(6836..7549)
                     /locus_tag="PLE23_06370"
     CDS             complement(6836..7549)
                     /locus_tag="PLE23_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82922.1"
                     /translation="MKSAFVALLTAFMFPLIFREIIEGHILLFFAFCSAVFLGSILLC
                     NADVGLCRSVKGFFKSYSHAPKDSETQISCKECSHLPLFGFIALLVCGISIFAVLLPW
                     LQTTELATMPIISIERNVEPTGIISGISLSGGIFGMLVAVVGGLLVLNQHKWSFMAGV
                     INAGIGVMYLNDFPVSQSLCISSSFAAGNGFISPSVLGSPQSGLYLFIGMSLLFTVLA
                     IGYTMDNDQPRLIHLGDVK"
     gene            complement(7687..>8270)
                     /locus_tag="PLE23_06375"
     CDS             complement(7687..>8270)
                     /locus_tag="PLE23_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82923.1"
                     /translation="LIFENDILEVISIPLKHRIPTSGFLFREKQRTPNIDKHKLEEYQ
                     LERAEILKLKAGESVQNRHGKHILPQDVLKAPLPKRSYAYCSDTMYAPENIPILKGVN
                     LLYHESTFLQVDEATALATNHSTAQQAASIAHDAEVGLLLLGHFSARYANRQYFIDEA
                     SQVFPNVFLASEGMVAKLEILDNKITTRLSNPA"
BASE COUNT         2595 a         1655 c         1629 g         2391 t
ORIGIN      
        1 cgttgaaata agcaggcact gttaccacag cctctgttac ttcctgaccg agataatctt
       61 cggccacttt cttcattttc tgaagagtaa tagcagaaat ttcctgtggt gaataaccac
      121 ggtcgtctat ttggattctg accgtatcgt tgtcaccttt tactactttg taaggcattt
      181 tggctgcttc tgctgtactt tcagaatacc ttgcgcccat aaatcttttg attgaataaa
      241 tcgtttttgt tgggttagtg attgcctgac gttttgcagg atctccaact tttctttcgc
      301 cattatccat gaatgctaca atagatggtg tggttctgcg tccttcgtca tttggaataa
      361 cgaccggctc ttttccttcc attactgcca cacatgaatt tgtcgttccg aggtctatgc
      421 caataatttt acccattgat tatgtattgt ttttaatttt ttaataatac aattgaataa
      481 tcaattggca tgccaccaca tatttatact taaaaatctg acaaaattaa cctttatgcc
      541 aaaatgttca agacaaattg acaatctgac agacaaaaag gcagctagaa ttactgaaat
      601 ccgagatcct ttgtataaat accactcttg agcgaatcgc gtgtaggtgt attaatcgaa
      661 taatcgactg cttcgagaat atttctggat gaaaaaatac ctataccatc attacttata
      721 ttggaataag taggcagaat ctgtgaactt gtgataccgc tgctggcatt acccaagtct
      781 atataatcct gaaattcctg tcctccggct actacggtta catcatagga aaggaatatt
      841 cgcttaatac ccggtatctt tgaaatgttt tctccaagaa atttaaaaat ttcttcgcct
      901 ttgagcattg ctgttatacg cgaggttggg tctgcaggcc tttgtacatt ttctgcaatc
      961 ttccaggtaa gtgatttttt tgcaaatttt gaaggttggt caattgtatt ttcattgtag
     1021 taaaacttca tgaacacatc atagactttg cctacagttt catttgaacg ccagctaaag
     1081 cggacatcag atttgtaagg ccatgcgccc ggatttggcg ggtctgttgc aaccatattg
     1141 taattgccta caacattggt tgtagcttcg gcaatagtcg tattattact cagatccttg
     1201 attgagagct tgtattgctc gccggctaca agttctatat cttttgtatt tattttgtac
     1261 agataattag gatcctgagc aaaagtaccc ggttttttag ggaatccttc atccgtaccg
     1321 tttacccttg taagtaccca tgtagtacca ttctttaccc tggtaaggct tacttcaata
     1381 ttattaaaat aaagcgaatc gggatttttt gctatctgtt cagcactttt ttcaggatcg
     1441 agaaatgctt tttctaccct tatgtattgt gcattttcag aaagattaag aaatccatat
     1501 acaaccggaa tacgcttcca aggagctacg aggtgcaatt cgttatcaca agaggagaat
     1561 gctacaatag cagccaacaa taatattatt ctgtttttca ttgggcaaaa ttagtggatt
     1621 tcaacaacta atgatataaa acttattttt tttaaattcc acactgattt tttgttgtgt
     1681 tacctgaatt tttgaataac tttcggccaa atttctaacc aaatcaaaca attctttcat
     1741 gtcagtagag aaacaaataa aacgagtgac ggttcataca ttgcaggcaa tgaaggagaa
     1801 ccatgagaaa atcagcatgt taactgctta tgattacagt atggccaagg tacttgatga
     1861 gtcaggtatc gacatattgc tcgtaggtga ctctgcctct aacgttatag ccggacacga
     1921 aaccaccctt cctatcacac tcgaccaaat gatttaccat gcagcatcgg tagtaagagg
     1981 tgttgaaaga gcacttgtta tagttgacct gccttttggt tcttatcagg gaaattcgga
     2041 gaaagcactt gattctacca tccgcattat gaaggaatcg ggtgcccatg gtgttaagct
     2101 ggagggcggt atcgagatag aagaatctgt aagaagaata ttatcggccg gggtgcccgt
     2161 gatggcccac cttggactta caccacagtc tatttataaa tttggcacct atactgtacg
     2221 tgccaaagaa gaagcagaag caaaaaaact gatggaagat gctttgctga tgcaggagat
     2281 tggttgcttt gcagttttga tggagaaaat acccgccgaa ttggctacca aagtaaccca
     2341 agcattgcac gtacctacca tcggtatcgg tgccggaggt ggagtggacg ggcaagtact
     2401 ggtaacacat gatttgctcg gccttacaaa agagtttaaa cctcgcttcc tgagaagata
     2461 tatggacctg tttgttgata taaaagctgc agtacaacat tatatcacag atgtaaaaaa
     2521 cgctgatttc ccaaatgaaa atgaacagta ttaaatgaag ctaaactttg aaggagcaca
     2581 aatgtggttc cgaaaagcga aaatatggtt attaaaccct gccaatcgaa agccggtata
     2641 catcataggt ggcgttttgg ctacaggcct tattgttttt atctggctgc ttgccaccct
     2701 gtttttatca aatgtaaaaa tagaagaagc aaaagctttc tacatttatg ataattatac
     2761 acttgattca atagccaaca agctacattc cgaacaactg atatccaatg aaggttcttt
     2821 tcgaacagct gccggtctgt tggggctcaa tgttaagaat atccgtgttg gtcgctataa
     2881 gattgaaccc ggatccggaa attttacatt ggtacgaatg tttaagaacg gccttcaaac
     2941 tcctgtaaaa attacgctga acaacataag aaccatcgaa gaactcgcag gaaaactcgg
     3001 ctcctacttg aagtctgact caactgcatt catgcacctt ttcagggaca ccacattcct
     3061 ttcaaaacac tatcttgact cagagactgt aatgactgca tttctgcccg atacatacga
     3121 aatgtattgg gacaccaaac ccgacgaagt ttttgaaaaa tttgcgaacg taacagttaa
     3181 attctggaac aaaaaaaggc ttaccaaggc cgattcgcta caactgactc cacaacaggt
     3241 atatacgctg gcttctattg ttgaaaaaga aaccaataaa gtggacgaac gccctgatgt
     3301 ggcaggcgtg tatctcaaca gatttcatca aaatatgcct ttacaagccg atccgaccgt
     3361 tgtgtttgca aacaatgatt ttacaataaa aagagtaacc ggtaagcaca ccgccattga
     3421 ttcgccctat aatacttata aatataccgg cctgccacca ggcccaatat gtatgcccga
     3481 gaagtcgagt atagatgccg tcctgaatgc taccaaccac gattatatgt ttttctgtgc
     3541 caagccagac ctttcgggtt accattcttt tgctgtgacc tttcagcaac atgtgcagaa
     3601 tgcaaaggct taccgcgatt ttttggacaa aaacggctac taatgatgtg gatgatgccc
     3661 acagaaaggc atttcttgaa agcgatggaa ggattcttcc ctgatggtgt gtcagacaaa
     3721 cgttttttgc tggctgtgag tggtggtgcc gattcgatgg ttgtgttgca tttgtttcag
     3781 aagcattatt ctgatttcat cattgcgcat tgcaattatg ggttacgtgg cacagaatca
     3841 gatcttgatg aggcattggt aaataaaatg gccaaagaat atgataaaat tttttgtgta
     3901 aaacacgttt cgctaccgca aaaggacaac aaatcaatcc aactgcttgc tcgtgaacat
     3961 aggtacgatt ggttcgctat gctaaaggac gaacatcagg tggattttgt ggtgacagcc
     4021 caccaccttg acgacaacac tgagacactg ctattcaatc ttataagagg caccggtatc
     4081 aaagggcttt gcggcattcc ggctatgagt gcaaacataa ttcgaccatt gctacaagta
     4141 agcaaaagta acattcttga atatgcgcag gcaaatggca ttcaataccg cgatgatgcg
     4201 agcaattttt caactaaata caacagaaat aaaatccggc ttaacatttt gccagaaata
     4261 gaaaaaataa atcctcagtt cagcaagact attaatgaac acatccgcga atatgccgat
     4321 cttgaagaat tactccgcga gcagactgca aattttatca aacaccactg tcgtaaggat
     4381 aatgactgtc ttgtcattcc aagtaaaacg ctgctgtgca aaccaggccg cttaaatata
     4441 cttttcagca tattaggtga atatggtttc agcaaaagcc aggttgaatt tttgctgaat
     4501 ggccagctca ctcgcagtgg cacaaaaata gaatctggca gtcatgaatt gtatctgcat
     4561 ggagatgaat ggatagttgc accggttttg tctgatttcg ccgaaataag cgtcagtcag
     4621 attccggcta cagtacacac agcttttggc tccttgagct ttatcgttgc gaccgaatcc
     4681 actaccaaaa cagaacggga cactttggtg ttcgaacata attttgacga caaagctttg
     4741 gtcatccgaa caagaaagga cggcgaccgc tttgcaccgg aaggaatgaa tggaaagagc
     4801 caaaaattaa aggatttttt catcaacagt aaaatatctg ttccggcaaa agcacgacag
     4861 cttttgttat gctcgggaga tgaaatcatc tggattgtcg gtttgcgcaa aagccaccac
     4921 acgcataaaa aagtaaataa tggaacattc gtacatgttg tattttcaaa aatcacctga
     4981 ccacatttaa catatcactg attttctaaa tattacattc caattttgta caagtcatat
     5041 caataattcc tgcataggta aaacttgtac gtacatcaat atttcaaaaa aacatcagac
     5101 ttgcattcac aatcaatgcg tactgttcgt tattccctaa agagaataaa aagctgaatc
     5161 ctaaaaaaaa ttaaccaatg aaaaaacaaa tattaattct gtttattctt ttacatgcca
     5221 cattgggtaa tggccaaaat ctgatttcga acgggcgatt tcaatatcct gaccttaatc
     5281 caaggtgcga cggttggttc aatttgtgtg gagaagaact gacagcacat tgtgacacaa
     5341 acctaaactg ttatgtcggt cttgtgattg attcgccatc ctggatacct gaagatattt
     5401 ggagtctcca ggtgaaagcc gcatttgagc ctgatggtta tgctgaaacc tacattacag
     5461 ggcaaaccgg taccaacata tacaccctca cctactggat gaatgccaaa gactgggcag
     5521 gaggagctag aatcggtacc ggaaagcaaa acaaattcgt tgagagccaa tctcgttctg
     5581 acacatgcag cagctggagt gaatttacat taaccgacac catcaccaca caggcaactg
     5641 atactataac agttagattg tcagccggtt cgggtgattt ttgtatttgt ccgcctatcc
     5701 gttttgcatt tattgagctt gttaagaaag ctattaacgc agttgatgat gtgaaccctg
     5761 tgcaaagcag catccttgcc tacccaaatc ctgctcagaa ggagataaaa tttgaagtaa
     5821 aaagtaaaaa ccaggggagc atgactttag agatattcaa cagcagtggt acacccatac
     5881 tatccaaaca atgcactgat ggattattct tggttaatag atcagagttg ccgggcggac
     5941 tgtatatata ttgtgtgaca tcagattcag atcaaataat tgtaggtagg ggcaaatttg
     6001 tatttgacta aattatcaaa ccttagtaat tgaaaaattg ccggaggcgt aataaagctt
     6061 acagtagatg gaaggaaata ttccgtcagg caaacatagc acaaggtaca aggcaggtta
     6121 atttttccga aaaagcaata cacacatttg gtattatgcg ttattaaata aagacttaaa
     6181 ctcgaaatct catcatcatg aaatcgaaac tactattttt aggcctgaca atcattctgt
     6241 taagttcttg ttgtaaagag aaaaattatt taggttcagg tgaatatgaa tattttgcat
     6301 tcggacactt ttttggagaa tgtggaggag aaaactgtgt tgtacggtat gtgattaccg
     6361 gcaatcaact ccttgaagat acggatgact ctgacaaaac agagaaattc gtaaagcttg
     6421 atcaggagtt gtactatcag gtcaaagatc tggaagacaa gattccacaa gagatgtttg
     6481 attcgccgca gaacgtttat ggctgccctg attgtgcaga tggtggtgga ctactcatag
     6541 aatacaggaa gaacggtaaa acacgcaaat gggcacttga ctataccaaa cacgagctac
     6601 ctgttcagat aggcaagttt gtggatgcaa ttcatgaaaa aatttacctt attaacaaat
     6661 gatatctacg tatatatctg ttaaagctct attcacgaac cacctaagag ccatattcta
     6721 catctattct tagatattgg tcaaaatagc acacattcat ataataccac agaagctagt
     6781 gccggcatag tgtaatggta aaggagctag cacgctaata atgtttcatt tcttgttact
     6841 tcacatcacc taaatgtatc aatcttggct ggtcattgtc catagtgtag cctatagcta
     6901 atacagtgaa caaaagcgac ataccaatga acagataaag acccgactga ggtgagccaa
     6961 gcacagatgg cgaaataaat ccattacctg ctgcaaatga cgaactgata cacaggcttt
     7021 gcgatacagg aaaatcattc aaatacataa cacctatgcc tgcattgatc actcctgcca
     7081 taaagctcca cttgtgttga ttcaaaacca gtaacccgcc aacgacagcc actaacatcc
     7141 caaatattcc tccggatagt gatataccac tgataatacc agtcggctcg acattccttt
     7201 caattgatat aatcggcatt gttgccaatt ctgttgtctg taaccatggt agtagtacag
     7261 caaatataga aataccgcag acaagcaatg caatgaaccc aaacaatggt agatgactac
     7321 attctttaca agatatttgc gtttcactgt ccttgggggc atgagagtag cttttaaaaa
     7381 aaccttttac actccgacac aaacccacat cagcattgca caataatatt gaacccaaaa
     7441 agacagctga acaaaaagca aaaaacaaca gaatatgccc ttctataatt tctcgaaaaa
     7501 tcagaggaaa cataaaagca gtaagcaatg ctacaaaagc tgatttcata atttcaaaat
     7561 tttatctgac agaaaagtga ttttcacaat cattaataca tctatcagta aaatcttaac
     7621 atcacaagcc aaattaaatt gaaaaaatat tggaataaat gatgttgaat ggaattggta
     7681 aaaatactaa gccggattac ttaatctggt tgtgatctta ttgtccaata tctccagctt
     7741 tgccaccatc ccttcgcttg caagaaagac gttggggaat acttgtgagg cttcatcaat
     7801 aaaatactgt ctgttggcat accttgccga gaaatgacca agcaaaagta agccgacttc
     7861 agcatcatga gctatagatg ctgcctgctg tgccgtagaa tggttagtag caagtgccgt
     7921 tgcttcatcc acctgcagaa aagtagattc gtggtaaagt agatttacac ctttaagtat
     7981 tgggatgttt tcgggtgcat acatcgtatc tgagcaatat gcataacttc tctttggtaa
     8041 cggggctttg aggacatcct gcggcagaat gtgctttcca tgcctgtttt gcacactttc
     8101 acctgctttg agtttcagta tttctgcacg ttcaagttgg tactcttcca gtttgtgttt
     8161 gtcgatattg ggtgtacgtt gtttttcccg aaaaaggaaa cctgacgttg gtatccggtg
     8221 tttgagtggg atggaaatta cctcaagtat gtcattttca aaaataagcc
//
LOCUS       DAOQQY010000103         8241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3391_length_8241_cov_6.044833, whole genome shotgun sequence.
ACCESSION   DAOQQY010000103 DAOQQY010000000
VERSION     DAOQQY010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8241
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3391_length_8241_cov_6.044833"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PLE23_06380"
     CDS             complement(<1..808)
                     /locus_tag="PLE23_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HMX82924.1"
                     /translation="MALEKYTDTYYLPLEGVHSEHCALIVDNGLGKIKQIGTHKIELN
                     NQRAVITTDNKEAVAEAIRAIKDLGYGVTTVRRTFPVLGMSCASCASSAESITAAQNG
                     VVDAAVNFATGNLTVEYLPNMINPTQLQEAIQSAGYDLLIAEDDSQQETLEAIHEKKF
                     SELKKRTTWAIILALPVMIIGMFFMHAPYANIIMFVLTTPIVFWIGRDFYINAWKQAR
                     HRSANMDTLVALSTGIAYIFSVFNMLFPQFWEERGLEAHVYFEAAAVIIAF"
     gene            983..3361
                     /locus_tag="PLE23_06385"
     CDS             983..3361
                     /locus_tag="PLE23_06385"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HMX82925.1"
                     /translation="MLTNQSTPDTILAGLNETDIAELRAKYGYNEIEQKKKPAILLLL
                     SKFWGLSAWMLEATVIISFALHKYFDGFVITFLLLFNGAIGFYNELKAARTVEALQQR
                     LQVMVRVLRNGAWQLLPSRELLPGDILRIRTGDFITADLTLKHGEVKTDKSALTGETA
                     LVTEKPEDKLYAGSVIKAGEGTAVVDAIASSSYFGKTVQLVIKSEHKLHMEEVVARVA
                     KMLFVSVLLILSLTTFIGLIGGQSLLSMLPLILILLVTAIPVGLPAMFTISMARGSRE
                     LSNKGVLVSKLSATEDAATLTTLCIDKTGTITKNELTLQDVTSFGTFTQRDVIRYGVM
                     ASVKANEDNIDLAFFDYIENNPSLTISAYQQLTFMPFDASRKHTEAVVQSEGKVMTVA
                     KGSFIAISGLCGKTEEIAKAKVSEWAQKGFKTIAVAVAFVERDFQFAGIVALYDPPRP
                     DAREMLAEIMNLGIGIRMLTGDALPIAREIAIQAGLGNNIISMNECRSDTDTDRLSET
                     INSHRGFAEVLPEDKYNIVKQLQQQGEIVGMTGDGVNDAPALKQAEVGIAVKNATDVA
                     KQSASIILIQEGLRQIIDLIKTGRTIHVNISNYTVNKIAKTILTILFVCTSFLITRTF
                     VVTTIDMVLMLFLIDFVVLTLAVDKVSWSQKPADWKIHRLVEKGILLGFLLFLGCTAG
                     FFGIKNLFDIQNEAMLHSLGFACLFFSAVMAIPVIRTDDTFYKAPISRLLLSVILADL
                     LLVMLLLTFGFPGFAKLPLEVSAITLVYFGVMMALVNDPAKVYFTEKMKYRK"
     gene            3741..4208
                     /locus_tag="PLE23_06390"
     CDS             3741..4208
                     /locus_tag="PLE23_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82926.1"
                     /translation="MNNNAGKTGLLVAEDHPYFNLNDLDRDKIYEQVEKLHFRITKEN
                     ATVKIKVGDAVGKLPDSRGELYILRNKDIRHVLSTNHKNRPARNNLFPNLKYLLSESK
                     LIMIVADDGKHSTWTKVWYYYLLTINDEDFFLNVYETLDGKYGIFSITDKIRN"
     gene            4351..4782
                     /locus_tag="PLE23_06395"
     CDS             4351..4782
                     /locus_tag="PLE23_06395"
                     /inference="COORDINATES: protein motif:HMM:NF016927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HMX82927.1"
                     /translation="MALTPVQFFKRLQASIPKLKHDILNDVIAVEAENFVSENFRKQA
                     YVDKGAKKWPARKDPKNKKALLIGTGTLRNQVTKARVSGDKVVIESSLVYAEVHNEGL
                     KAGRGEGFMMPKRQFIGESATLTKRIGKKAAQLLDSHLNNL"
     gene            4812..4988
                     /locus_tag="PLE23_06400"
     CDS             4812..4988
                     /locus_tag="PLE23_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82928.1"
                     /translation="MKDVFLTVKDKVATVEEVRFYDWDLGQLNDTPPPVSWPVVLFDY
                     NEALVEELSDEPIL"
     gene            complement(5359..6741)
                     /locus_tag="PLE23_06405"
     CDS             complement(5359..6741)
                     /locus_tag="PLE23_06405"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82929.1"
                     /translation="MKRVITLLLISLCSGIILHAQKWQKQFPAFTKASQSLKVDIVDK
                     YNAWALAMPIADAPQYFLPGNYYTRTTDGGETWVTNALPSGYSGDLSNLTAISADKAW
                     IGLQDYQSDTNGVAMVFRTKDGGQNWEKLNLQLNSYINMVHFFDENDGIVMGDPIGDE
                     FQIFTTSDGGDTWQQVPSQDMPKAIDSGELGLNFDSYGTNANSLVFDTYYNRLFVTRD
                     RGKHWGILEPSISGEGLTWFPHLDEDNNLYLPLYYPPKDTTDVWGATYMLQKYNLDDD
                     TSWTDITPVDNKYYVSGFSSIPDGPTIVMNTVFKTLVSYDRGVHWLELSTTTTPFGFM
                     SFYDSGIGYSVKLPDLTDRLNNPCDEFYKYNGSPLTGLIHQKPLDAQISIGPNPVMSV
                     LNINVKTAEAQHYAILIHDLSGKLFKTMETDKLSTIGVSVNVQDLPAGAYTLSISCQT
                     GSKTIPFVKQ"
     gene            complement(7264..>8241)
                     /locus_tag="PLE23_06410"
     CDS             complement(7264..>8241)
                     /locus_tag="PLE23_06410"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMX82930.1"
                     /translation="VESATHAVGPFVVMFGFSLALALPFGLFAAFPAWLNSLPKSGSW
                     MTSVKVVLGFLELALAFKFLSVADMTSHWGILKYELFMALWVIIFAGMTIYLFGKIRF
                     PHDSPIKKLSNTRKALASLSLIWTLYLVTGFFYNSANASYDSKLLLSGLAPPAHYNYF
                     LEEGKLDPAIKAKYPSYSKAANNLNCFKDYCEGLAYAKEVGKPVLLDFTGYGCVNCRK
                     TEEHIWVNDRVRKMIDEDYVLISLYVDDKQKLDEMLYSTSREEKLRDVGDVWADLQIV
                     NFKQNSQPLYVLMTPDEKVLAKPRGYKEGVDEYADYLKCGLNTYSTVKN"
BASE COUNT         2566 a         1635 c         1749 g         2291 t
ORIGIN      
        1 taaaggcgat gatgacggct gctgcttcaa agtaaacgtg tgcttcgagc cctcgctcct
       61 cccagaattg tggaaaaagc atattaaaca cgctaaaaat ataggcaata cccgtactga
      121 gtgccaccag cgtgtccata ttggcggaac ggtgccttgc ctgtttccat gcgttgatat
      181 aaaaatcgcg tccaatccag aagacgatgg gtgtagtcag cacgaacatg atgatattgg
      241 cataaggtgc atgcatgaaa aacataccga tgatcatgac cggcagtgcg aggatgatag
      301 cccatgtcgt ccgcttcttc aactcgctaa attttttctc gtggatggct tccagcgttt
      361 cctgctgcga atcatcttcg gcaatcagca ggtcgtaacc ggccgattga atggcttcct
      421 gtaattgggt agggtttatc atattcggca ggtattccac ggtcaggttt ccggtcgcaa
      481 aattgactgc agcatccact acaccattct gagctgcggt aatgctctcg gcgctggacg
      541 cacatgatgc gcacgacatg ccgagtaccg ggaaagtcct acgtacagtg gtgacgccat
      601 agccgagatc tttgatggcc cggatggctt cggcaactgc ctccttatta tctgtggtga
      661 tgactgcgcg ctggttgttg agttctatct tatgagtgcc gatttgtttg atttttccaa
      721 gcccattatc gacgatcaaa gcacaatgtt cggaatgaac accttcgagg ggcaggtaat
      781 aggtatcggt atatttttct aaagccataa aaatctttat ttaaaatgaa taataaaaaa
      841 tgctgtatta agttcatttg aagggttttg tgcaagtggc attattattt taataaatga
      901 catacagcca aaaacgaaaa atatcattta taaaggataa tcaaacgtca gataaagctt
      961 gtgttttagt ttaaatttgt cgatgctaac aaatcaatcc actccagaca caatacttgc
     1021 cggcttaaac gaaaccgaca ttgctgaatt gcgtgccaaa tatggctata atgagataga
     1081 acaaaagaaa aaaccggcca tattgttgct gctgagtaaa ttctggggac tgtctgcctg
     1141 gatgctggaa gcgacagtta ttatatcgtt tgcactgcac aagtactttg acgggttcgt
     1201 catcacattt ttgcttctgt ttaacggtgc gataggattt tataacgaat tgaaagcggc
     1261 aagaactgtg gaagctctac agcagcggct tcaggtaatg gtaagagtat tgcgcaacgg
     1321 agcctggcaa ctccttccat caagagaact tttaccggga gatatactgc gcataaggac
     1381 aggtgatttt attaccgctg acctaacatt aaagcatgga gaggtgaaga ctgacaaatc
     1441 ggcactgacc ggtgaaactg cacttgtgac cgaaaaaccc gaagacaaac tgtacgccgg
     1501 ttcagtgata aaagctggcg agggcacggc agtagtggac gcaatagcct cttcaagtta
     1561 ttttggcaaa acagttcagc tcgtcatcaa atcggaacac aaactgcaca tggaagaagt
     1621 agtggccaga gtggcaaaaa tgctatttgt atcggtattg ctgatattgt cacttaccac
     1681 cttcattggg ttgataggcg gacaatcatt actgtcgatg ctgcccctga tactgatact
     1741 tttggtaaca gctattccag ttgggcttcc ggccatgttt actatcagta tggcaagagg
     1801 ctcgcgagaa ttgtctaata aaggcgtgct ggtaagcaaa ctcagcgcta ccgaagatgc
     1861 tgctacgcta accaccttat gcattgataa aaccggcacc atcaccaaaa acgaacttac
     1921 gctgcaggat gtaacttcat ttggcacatt tacacagaga gatgtcatcc gatacggtgt
     1981 gatggcatcc gttaaggcca atgaagacaa cattgatctt gctttttttg attatatcga
     2041 gaacaaccct tcgttaacca tatctgcata ccaacaattg actttcatgc cctttgatgc
     2101 ttctcgaaag cacacagagg cagtcgtaca atcagaagga aaggtaatga ctgttgccaa
     2161 aggatcattt atagccatca gcgggctgtg tggaaaaaca gaagaaatag cgaaagcaaa
     2221 agtgtcggaa tgggcacaga aaggtttcaa aaccatagct gtggcagtgg catttgtgga
     2281 gagagatttt cagtttgccg gcatcgttgc cctatacgat cctccacggc cggatgcccg
     2341 tgaaatgttg gcagaaataa tgaatctcgg catcggtatc agaatgctca ccggtgatgc
     2401 gctgcctata gccagagaaa tagctataca agcaggtctg ggtaataaca tcatttccat
     2461 gaatgaatgc aggtcggata ccgacacgga cagattaagt gaaaccatca acagtcacag
     2521 aggctttgcg gaagtcttgc ctgaagacaa gtacaacatc gtgaagcagc ttcagcaaca
     2581 aggcgagata gtaggaatga ccggtgacgg ggtcaacgat gctccggcac taaaacaggc
     2641 tgaggtgggt attgctgtta agaatgccac agacgtagcc aaacagtcag ccagtatcat
     2701 ccttatacag gaagggctaa ggcaaattat tgaccttata aaaaccggtc gcactatcca
     2761 cgtcaatatt tcgaattaca ctgtaaacaa aatcgccaaa accatcctaa caatattgtt
     2821 cgtatgtact tcgttcctga taaccaggac ctttgtggtc acgaccatcg acatggttct
     2881 gatgcttttt ctgattgatt ttgtggtgct gacgctagcg gttgacaaag taagctggag
     2941 ccaaaaacca gcagactgga aaatacacag gctggtggag aagggcatct tgctcggatt
     3001 tttactattc ttaggttgta cagcaggatt tttcggcatt aagaatctat tcgacataca
     3061 gaatgaagcc atgctgcact cgttggggtt cgcttgcctg tttttttctg cggtaatggc
     3121 tatacccgtc atccgcactg acgatacatt ttacaaagca ccaataagca ggttgctatt
     3181 gagtgtaatc cttgctgact tactgttagt catgctatta ctgacattcg gtttcccggg
     3241 ttttgcaaag ctacctttgg aggtcagtgc tattaccctg gtatattttg gagtgatgat
     3301 ggcactggtc aatgatcccg ccaaggtgta ttttactgaa aagatgaaat acaggaaata
     3361 aaaatatagt acaagatgtg attgtgcaag ctcaacagca ttaggtttat taaacagaaa
     3421 gtctggctat aataggcgat aaactggtat aactaaagcc agataacgca ttagcctctt
     3481 tactgcaaat aattattact tcaaatcatt ttcgtttaaa ttcctaatgc gttatctggg
     3541 tttattgaaa gaaataaaaa cccacattga cggtaaacat caatgcgggc cataatatga
     3601 aaaaattgct cagtttattg ttttacgaat ggtattgttt tacttcctgt ctgacaactg
     3661 atacttaaag tataagcgcc tgccggcaga tcttgaacat ttaccgaaac accaatggtg
     3721 atattaagat cagtaagggc atgaataaca atgccggcaa aaccggtttg cttgtagcag
     3781 aggatcatcc gtatttcaac ctgaatgact tagacaggga caagatctat gagcaggtag
     3841 agaagttgca ttttaggatt acaaaagaaa atgcaactgt aaaaataaaa gttggtgatg
     3901 ccgttggcaa attgcctgat agtagaggcg aactatatat tttaaggaat aaagacatta
     3961 ggcatgtttt atctactaat cacaaaaaca ggccggcaag aaataatttg tttccaaatt
     4021 taaaatacct tttatctgaa tccaaattga taatgatcgt tgctgatgat ggtaagcact
     4081 caacttggac caaggtttgg tattactatt tacttacaat aaatgacgaa gacttttttt
     4141 taaatgtata tgaaacttta gacgggaagt atgggatttt ttcaattaca gacaaaatca
     4201 gaaattaaaa aaggccttga tgacacccaa tctgcaatcg ggattaacca agacctttaa
     4261 gatcaacaac gagaccggaa taagccggct gatgaaaatc tcattacaaa gataatcact
     4321 ttttatttaa aaacaaaaaa atattaaaaa atggcactta cacccgttca gttctttaaa
     4381 agactgcaag caagcattcc taagctgaag catgacatac ttaatgacgt catcgctgta
     4441 gaggctgaaa acttcgtgag cgagaacttc agaaagcagg cttacgtgga caaaggggca
     4501 aaaaaatggc ctgcgagaaa agacccaaaa aacaaaaaag cactactgat aggcaccggc
     4561 acacttcgca accaggtgac taaagcccga gtgtctggtg ataaggtagt cattgaatca
     4621 agcctcgttt atgctgaagt acacaatgaa ggactgaagg ccggaagagg agaaggattc
     4681 atgatgccga agcggcagtt cataggtgaa tcggctacgt taaccaagcg tataggcaag
     4741 aaagcagcac agctgttaga ctcgcacctt aacaacttat aacccattta taaaccattt
     4801 acaaacaatt tatgaaagac gtattcttaa ccgtaaaaga taaagttgcc actgtagagg
     4861 aagtaagatt ctatgactgg gatcttggcc aactcaatga tacaccacca ccggttagct
     4921 ggcctgtggt attgtttgat tataacgaag cgttggtgga ggaattgtcc gatgagccga
     4981 tactatagaa gggttttgtt ttagtgtgaa agtgcaagat ctagtcatca acattatctt
     5041 caaacccaag aacgatatac ctagctacag caataaccgt tccggtatat gaactacggt
     5101 cattgctcgt gctttgggag ccgtttttac caccctaatt ttcataaaat tcaattctta
     5161 gtgcggtaaa agcaataaat ttaaatctca aatcattgat tataaatatt ttaaaaccgc
     5221 aataaatcta aagggtacaa gttataaatc aatattttca cacaataaag aggctggcaa
     5281 ataattaatt gaaagaaata aaaacccaca ttgacggtaa acatcaatgc gggccataat
     5341 atgaaaaaat tgctcagttt attgttttac gaatggtatt gttttacttc ctgtctgaca
     5401 actgatactt aaagtataag cgcctgccgg cagatcttga acatttactg aaacaccaat
     5461 ggtgctaagt ttgtcggttt ccattgtttt gaataatttg cctgataggt catggatgag
     5521 aatcgcataa tgttgagctt cagctgtttt aacattgatg ttcaaaacac tcattacagg
     5581 atttggacct attgatattt gagcatcaag tggtttttgg tgaataaggc ctgtaagtgg
     5641 tgagccgtta tatttataga actcatcaca agggttgttt aacctgtcag taagatccgg
     5701 taattttaca gaatacccga tacctgaatc gtagaatgac atgaacccaa aaggtgtagt
     5761 ggtggtgctt aattcgagcc aatgaacacc cctgtcatac gataccaagg ttttaaaaac
     5821 agtattcata acgatggtcg gcccatccgg aatactacta aaaccactta cataatactt
     5881 attgtcaacc ggggtaatgt ctgtccagct ggtatcatca tccaagttat atttttgcaa
     5941 catataggta gcaccccaga catctgttgt atcttttgga gggtaatata aaggaaggta
     6001 caggttgttg tcttcatcaa ggtgaggaaa ccacgttaat ccttctcctg agatggaggg
     6061 ctccaaaatt ccccaatgct ttcccctatc acgtgttaca aacaggcggt tatagtacgt
     6121 atcaaatacc aaagaattgg cattggtacc ataactgtca aaattcagac ccaactctcc
     6181 gctatctatg gcttttggca tatcctggct gggtacctgc tgccaagtat ctccaccatc
     6241 tgatgttgtg aatatctgga attcatctcc aattgggtcg cccataacta taccatcatt
     6301 ttcatcaaag aagtgaacca tgttgatata tgaatttaac tggaggttta gtttttccca
     6361 attctgacca ccatccttgg tacggaatac catcgcaaca ccattggtat ctgattggta
     6421 atcctggagc ccgatccatg ccttatcggc tgatattgcc gtcaggttag aaagatcacc
     6481 actataacca gaaggcaaag cgttggttac ccacgtctca cctccgtcag tggtacgggt
     6541 atagtaattg cccgggagga aatattgtgg tgcgtcagca attggcatag ccaatgccca
     6601 ggcattatat ttatcgacaa tatcgacctt gaggctttgg cttgctttgg tgaaagcagg
     6661 gaattgtttc tgccatttct gtgcatgtaa aataatacca ctacaaagtg aaatcagcaa
     6721 aagtgtaatt actcgtttca taattttcaa aatttaagaa gtgaacaata ttgggtttaa
     6781 gagaattttt gttttgtact tttacctaaa tattaaaatt accaatcata acatacttag
     6841 caattgttta aaaaatgaag tctttgcact tacgctacaa attatgtcac cccttttctc
     6901 cttaagtgat taacctccca acactcatac attaataaca aatacttatt ttccttaaca
     6961 tcaaaagcaa gaaattttaa aaaaaaattc ttcactgctt ttaaagatgg ggaatgacca
     7021 actctgcggc acctatttta acccagaaaa agcgtaagga attcaaccga aaataattgg
     7081 tacaaaaatc cataaaaggc ataagaagca aagtgaagta tgattgatga gcttttgcaa
     7141 tggatttggt agaaaagaaa attacagtat agcgtcttaa gcgttatata ggttaaaatg
     7201 aataaaaaaa atccggatgt tatacaagca tccggatttt tagttttaac accttggtta
     7261 ttgttaattc tttactgtcg aataagtatt taacccacat tttagataat cggcatattc
     7321 atctacacct tccttataac cacgtggttt tgcaagcact ttttcatccg gtgtcatgag
     7381 cacatatagt ggctgcgaat tttgtttgaa gttaacgatc tgcagatctg cccatacatc
     7441 gcctacatcg cggagttttt cttctctgga tgttgaataa agcatttcat caagcttttg
     7501 tttatcatct acatacaatg agatgagcac ataatcttcg tcaatcattt ttcgtacacg
     7561 atcgttcacc cagatgtgtt cttcggtctt tcggcaattg acacaaccat agccggtaaa
     7621 gtcgagcaga accggtttgc ctacttcctt ggcataggca aggccttcgc aatagtcctt
     7681 gaagcagtta aggttgttgg ctgcttttga atatgaagga tattttgctt taatagcagg
     7741 gtcgagttta ccttcctcaa ggaaatagtt gtagtgggct ggtggtgcta agccactcag
     7801 caaaagtttt gaatcgtaag atgcatttgc agaattatag aagaatccgg taacaagata
     7861 aagtgtccag atcaatgaaa gggatgccag cgcttttctt gtattgctca gttttttgat
     7921 cgggctgtca tgcggaaacc taattttgcc aaataggtaa atagtcattc cggcaaaaat
     7981 aattacccat agggccataa acagttcgta cttaaggatg ccccagtggg atgtcatatc
     8041 agccacagaa agaaatttga aagcaagtgc cagttcgaga aaaccgagta ccacttttac
     8101 agaggtcatc caactaccgg acttgggtag cgagttaagc catgccggga atgcggcaaa
     8161 tagtccgaaa ggaagtgcaa gtgcaagtga aaacccgaac atcacaacga agggtccgac
     8221 agcatgagta gctgactcta c
//
LOCUS       DAOQQY010000104         8231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3398_length_8231_cov_5.299413, whole genome shotgun sequence.
ACCESSION   DAOQQY010000104 DAOQQY010000000
VERSION     DAOQQY010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8231
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3398_length_8231_cov_5.299413"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PLE23_06415"
     CDS             <1..458
                     /locus_tag="PLE23_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMX82931.1"
                     /translation="YKYFDIKATPAKETARDVVNISFKKMCTEFAKYAANPLNKGGLW
                     ANYKNTTIEHLGKIPAFGRKDIKVDGFKDAINATTEYNGPSWRMVVQLSKPVRAYGIY
                     PGGQSGNPASKYYDNMIDKWVNGQLDELVFMRNGEETDKILYTIELTNK"
     gene            462..830
                     /locus_tag="PLE23_06420"
     CDS             462..830
                     /locus_tag="PLE23_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82932.1"
                     /translation="MKNLSKFIIITAACLLVQLYFPWWSALIVLAVFSALLRISPGTA
                     FAFGSGILGPMWMIYAIFLNQTNDGILAARIGELFQGIKASNLIQISTTLATLMGGLS
                     AMTGSLFMNMFRKNQRTHTV"
     gene            1302..3431
                     /locus_tag="PLE23_06425"
     CDS             1302..3431
                     /locus_tag="PLE23_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HMX82933.1"
                     /translation="MTDNIHIDLIAQSTGILARFVSNTIKLLTEGNSIPFISRYRKEM
                     TGSLDEVAIGNISKQLDKLRELEKRKQTVVAAIEEQGKMTDALLKRITNCYDTTELED
                     IYLPYKKKKKTRAMIAVENGLEPLAQLLSKQTFTDIAREATAFITDKVPTAEDALSGA
                     RDIIAEWINENEEARQKIRVLFKKSAILSTSLIKGKEAEAQKYKDFFNSSEPLAKCPS
                     HRMLAVRRAEAEGYIRVSISPSMEDAILILERIFVKNNSDSALQVKLALQDSYKRLME
                     PSIETEFRNLSKEKADEEAIKVFANNLMQLLLQAPLGNKAIMGLDPGFRTGCKLVCLD
                     NNGQLLRNSTIYPHPPQSDTKGAAKEILHLVALFDIEAIAIGNGTAGKESFNFVKNLD
                     FGRPVEIYLVNESGASIYSASEVAREEFPDQDVTVRGSVSIARRLQDPLAELIKIDPK
                     SIGVGQYQHDVDQAELKRSLDQTVINCVNSVGINLNTASKHLLTYVSGVRPVLAKNII
                     AYRNKAGGFTDRRQLLEVPRLGPAAYEQAAGFLRVVNPENPLDNTAVHPESYHIVEKM
                     AHDLSVSVEDLIGNPVLRKKIDIREYVTEKTGLPTLQDIMKELEKPGLDPRGNASPVD
                     FKDNINSMDDIEVGMVMTGIVTNLTKFGAFVDIGIKQDGLVHISQIADKYIKDPADVL
                     KLNQRVQVKVLEIDRERKRIHLSIKHA"
     gene            3440..5845
                     /gene="pbpC"
                     /locus_tag="PLE23_06430"
     CDS             3440..5845
                     /gene="pbpC"
                     /locus_tag="PLE23_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HMX82934.1"
                     /translation="MSVRINTPTILAYIKRRKILTACLVVAILLYAFCLPNPLFRQPV
                     STVLEDRHGQLLGAKIAADGQWRFPEMDELPEKYITALTSYEDKRFFYHPGFDPLSII
                     RAIRQNLASGKKVSGASTITMQTIRLARGRKRRNIPEKIYEIILATRLEWRYSKQKIL
                     KLYAANAPFGGNVVGLETACWRYFGKNIHDLSWAEACMLAILPNNPGSIFPGKNRQTL
                     LLKRNKLLKKLYELNKIDKTTYELSTEEILPDAPTTLPRWTPQLVEKFAKQTQQNEQR
                     KKIHTTIDLELQKKVNDIARRHTELLRINDIQNLAILIIDNKSNEVLSYTGNAPDAGI
                     NNQGDVDIITASRSTGSLLKPLLYAKAMDRGLVLPHSLLPDIPVQFGAYKPENFRLTY
                     DGTLPVNLALAKSLNVPFVKLLESYGIQVFHNDLKKFGFTGLNPSASHYGLTMVVGGI
                     ENSLWNVCKCYAGMSRVLSDFRKNNGKYSLHAFDEPSIVGHEPIPKGEKLSKYAPVVK
                     AASVWHTFEAMQGLERPNESGDWEKFVSSRKIAWKTGTSFGYRDAWAVGVSPRYTVGV
                     WAGNATGEGRPNLIGIYTAAPLLFEIFDQLPDAPWFEEPYDDEVKIPVCRTSGYRASA
                     YCPADSVFVPSAEIHSAVCSYHEQIYTDLRGEYRVNMDCETGAGMEAKSWFVLKPIEE
                     YYFRQKNSWYEPLPPYKEGCNPSIRGRELINIIYPRDESVVFLPRQMSGEKGQVILEA
                     AHQRSDAAINWFIDEQFMATTHQKHKINVSPTPGQHLLVLVDDKGYRLVIRFEAKDSE
                     SKR"
     gene            complement(6264..7529)
                     /locus_tag="PLE23_06435"
     CDS             complement(6264..7529)
                     /locus_tag="PLE23_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX82935.1"
                     /translation="MNIGIFTYGTRGDLQPYVALALGLVDKGHKVTISATDDFKAFVE
                     GFGVPFQPLWGNAEKMMNSTEGQSILQTENAIKLMRYYFKVLHDNREPLRKSYFEAIS
                     KVDFIIANSMTLPIGSAIAEKQNKKVALTYFMPPVVPTIEFPLGDFDFFNFPWYNKLT
                     FKIAQSFFWKFIKQDTNEYRQELGLPILKENLVTYLDKQKILDLYCISQSLIPQPKDW
                     ESHHKITGFIYIPKHKRDEHFLDKTPTELTEWIDQGDKPIYIGFGSNGVGNTEKFIKI
                     LTEILVQTNERILFCKGWSQFDNLPEHKNLFVTKYVNHETILPKCKIGIFHGGAGTLA
                     TMLRHNLPVIIVSFYTDQPTWGKIVERKKLGVHIPVKTLSADKLISAIQKVQAKEIEN
                     NVSIAGQQISNENELDNALSEIEKYFNDK"
     gene            complement(7582..>8231)
                     /locus_tag="PLE23_06440"
     CDS             complement(7582..>8231)
                     /locus_tag="PLE23_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMX82936.1"
                     /translation="VFVFLVKFLKYFFHRLTFCAILQKRSKLMNIFIEYLTSISKLSN
                     SAIHYINEYLSVEKIPKKQVFVQELSASKKLYFIKKGIVRAFFYNDGKEITDWFGIEN
                     MIIGATVRNFPIKNTRHSIETLEDTELVSVSFNDLEKLYQKHHDIERLGRLIAINTIL
                     FLQHKIDTQQLFSAKERYDEFVYKYPNLINRVPLGYIASYLGMNQVTLSRIRNQY"
BASE COUNT         2665 a         1625 c         1684 g         2257 t
ORIGIN      
        1 aatacaaata ctttgacata aaagctacac ccgccaaaga aacagcaaga gatgtggtga
       61 acataagctt taagaaaatg tgtaccgaat ttgccaaata cgcagccaac ccgctaaaca
      121 aaggcggtct atgggcaaat tataagaaca caacaataga acatttaggt aaaatacctg
      181 cattcgggcg aaaagatata aaggttgacg gattcaaaga tgccattaat gctactacag
      241 aatacaacgg tccgtcatgg agaatggtcg tacagctctc aaaacctgta agagcttatg
      301 gtatttaccc gggagggcag tcgggcaacc cggcaagcaa atactatgat aatatgatag
      361 ataaatgggt caacggacag ctagacgaac tagtgtttat gcgcaatggt gaagaaactg
      421 ataagatatt atacaccata gaattaacta ataaataagt gatgaaaaac ctgagcaaat
      481 ttataatcat tactgcagca tgcttgctgg tacagctata ttttccatgg tggtcagcac
      541 tgattgtcct tgcggtattc agtgcattgt tgcgcatcag tccgggaaca gcttttgcat
      601 ttggctcggg tattctgggg ccgatgtgga tgatatatgc tatttttctc aaccaaacga
      661 atgatggtat tcttgcagcc agaatcgggg agttattcca aggaataaag gcatcaaatc
      721 taatacaaat aagtactacc ttggcaacat tgatgggtgg cctgtcggct atgaccggaa
      781 gtctttttat gaatatgttt agaaaaaatc aaagaaccca tacagtttag atattattat
      841 tttaactaat atcactcatc tgtaattttt acagaattta taaacgattg acaatcattt
      901 atttagaaaa taattaaaat cgaattcaag atttaattta tacttattaa aaaatttttg
      961 aaaaattgtt ttgtagaata aattaatgtt cctaacattg catcgttatt caaacagact
     1021 catcaattag gcgcgctatg agaaaacaaa atgactggcc attgccaaaa agccgattga
     1081 tagtcccata acatactttc cttgaacaat caacaataac atacttactg agaggccatc
     1141 cttgttcttt attctttccg gtcatataat tttatttatg gcagggaagc caaaaccgat
     1201 ttaaactact cgttagagtg aaggttacca ttcccttttg gtaacctttt ttattttgta
     1261 tcaaatgtgt tgtaacagaa agtgtttagt aattttgcag aatgacagat aacatacaca
     1321 ttgacctaat cgctcaaagt acaggcatac ttgcacgctt tgtatcgaat accatcaaac
     1381 tactcaccga aggcaattcg attcctttca tatcgcgcta tcgcaaagaa atgaccggtt
     1441 cacttgatga agtagccatt ggcaatattt cgaaacaatt agacaaacta cgcgaactgg
     1501 aaaaacgaaa acaaaccgta gtagcagcaa tcgaagaaca aggcaagatg accgatgctc
     1561 tattgaaacg cataaccaac tgctatgaca ccaccgagtt ggaagacatt tacctgccct
     1621 ataaaaagaa gaaaaagaca cgtgctatga tagccgtaga aaacggcctc gaaccactcg
     1681 cccaactact ttccaaacaa acttttactg acatagcaag ggaagccaca gcattcatta
     1741 cagacaaagt gcctactgct gaagatgctc tatcgggtgc aagagacatc attgccgaat
     1801 ggatcaacga aaatgaagaa gcacgccaaa aaataagggt tttgtttaaa aaatcagcta
     1861 ttctgtccac ttctcttatt aaggggaaag aggctgaagc ccaaaaatac aaggattttt
     1921 tcaacagtag cgaaccactg gcaaagtgtc cgtcgcacag aatgctggct gtacgcagag
     1981 cagaggcaga aggctatata cgtgtgagta tcagcccttc gatggaggat gccatcttga
     2041 tcctggagag aatatttgta aaaaacaatt cggactctgc acttcaggta aaactggcat
     2101 tgcaagacag ctataaaagg ctcatggaac catccattga aaccgaattc agaaatctgt
     2161 caaaggagaa agccgacgaa gaagccatta aggtctttgc caacaatctt atgcagctat
     2221 tgctacaagc accactaggt aataaagcca tcatggggct cgatcctgga ttcaggacgg
     2281 gttgcaagct tgtgtgcctt gacaacaacg gccaattgct ccgcaatagt accatttatc
     2341 ctcatcctcc acagtcagat actaaaggag ccgcaaaaga gatattacac ctggtggctt
     2401 tatttgatat tgaagcgata gccattggca atggtacagc aggcaaagaa agtttcaatt
     2461 ttgtaaaaaa tctggatttt ggtcggccgg tagaaatcta tcttgtcaat gaaagtggtg
     2521 catctattta ttcggcatct gaggtggcac gcgaagaatt tcctgaccag gatgtgacgg
     2581 tgcgtggctc tgtctctatt gcgagaagac tccaggaccc actggccgaa ctgataaaaa
     2641 tagacccgaa gtcgataggt gtagggcaat atcagcacga tgttgaccag gctgagctaa
     2701 aaaggagcct tgaccagacg gtcatcaatt gtgtaaacag cgtgggtatt aatctgaaca
     2761 ctgccagtaa acatctgctg acatacgtgt cgggtgtgag gcctgtattg gccaaaaata
     2821 tcattgcata ccgtaataaa gcaggcggat ttactgaccg cagacaacta cttgaagtac
     2881 caagactagg tccggctgct tatgagcaag ctgccggttt cttacgtgtc gtcaatcctg
     2941 aaaatcctct ggacaacact gctgtgcacc cggagagtta ccatattgtc gaaaaaatgg
     3001 cacacgattt atcggtgagt gtggaagatt tgattggcaa tccggtattg cgtaaaaaaa
     3061 tagacatcag agaatatgta acagagaaaa ccggtctgcc cacactgcaa gacattatga
     3121 aggaactcga aaaaccaggt ctcgacccaa gaggcaatgc aagcccggtg gactttaaag
     3181 acaacatcaa cagtatggac gacatcgagg tcggaatggt gatgaccgga atagtcacaa
     3241 atctgacaaa attcggtgct tttgtcgata tcggtatcaa acaagacggg ctggtacata
     3301 tatctcagat agctgacaaa tatatcaaag acccggctga tgtgttaaag ttgaatcaaa
     3361 gagtacaggt aaaggtactg gagatagacc gtgagcgaaa acgcatccat ttatctatca
     3421 aacatgcctg atctgtatca tgtctgtcag gatcaataca ccaaccatac tagcctacat
     3481 aaaaaggaga aaaatactga cggcatgcct ggtagtagct atactcttgt atgccttttg
     3541 tttgccaaat cctttgttca gacaaccggt aagtacggta cttgaagacc gacacggaca
     3601 actgcttgga gcaaaaatag ctgctgacgg gcaatggcgt ttccctgaaa tggacgagct
     3661 gccggaaaaa tacatcactg cgctcacaag ttacgaagac aagcgttttt tctaccatcc
     3721 gggcttcgat ccgttgtcaa tcataagagc catacggcag aatctggctt cgggtaagaa
     3781 agtgtctggt gccagtacta taactatgca aactatacgc ctggctcgtg gtaggaagag
     3841 aaggaacatc cctgagaaga tctatgaaat aatattggca acaaggcttg agtggcgtta
     3901 ttcgaagcag aaaatcctga aactatatgc agcgaatgca ccattcggag gaaatgtagt
     3961 aggacttgaa accgcttgtt ggcgatattt cggtaaaaac atccacgacc ttagctgggc
     4021 agaggcttgt atgctggcta tactacccaa caatccgggt tcgatctttc caggcaaaaa
     4081 ccgacagaca ttgttgctca aacgaaacaa gctgcttaaa aaactttatg aacttaataa
     4141 gattgacaaa accacatacg aactgtcaac agaagaaatt ctccctgacg cccctactac
     4201 cctgcccaga tggacacccc agcttgtaga gaaatttgcg aagcaaacac agcagaacga
     4261 gcagagaaaa aaaatacata ctaccataga cctcgaactg caaaaaaagg taaacgacat
     4321 agcccgcagg catacagaac tactaagaat caacgatata caaaacctgg ccatactgat
     4381 aattgacaat aaaagcaatg aagtacttag ctataccggc aatgcacccg atgcgggcat
     4441 caacaaccaa ggagatgtgg atatcataac agcctcacgc agcaccggca gcttgctaaa
     4501 acctctactg tatgccaagg caatggatcg cgggcttgta ctaccccact cactgctgcc
     4561 tgacatacct gtacaatttg gtgcctacaa acctgaaaac ttcagactaa cctatgacgg
     4621 aaccttgccg gtcaatctgg cgcttgccaa atcgctcaat gtgccgttcg taaagctgct
     4681 cgaaagctac ggcatacagg tatttcacaa tgatcttaag aaattcggat ttacgggtct
     4741 gaatccgtct gcctcacact atgggctaac aatggtagtg ggtggcatcg aaaacagcct
     4801 ctggaatgta tgtaaatgtt atgcaggcat gtcgagagta ctgagcgact tcaggaaaaa
     4861 caacggaaaa tattcgctgc atgcatttga tgaaccaagc attgttggtc atgaacctat
     4921 accgaagggc gaaaagctca gcaagtatgc gccggtagtg aaagcagcct cagtgtggca
     4981 tacctttgaa gctatgcagg gacttgagcg gccaaatgag tcgggcgact gggaaaaatt
     5041 cgtgtcttcc agaaaaattg cctggaaaac cggcacaagt tttggctacc gcgatgcctg
     5101 ggctgtaggt gtcagcccgc gttatacggt aggtgtatgg gcaggcaatg ctaccggtga
     5161 aggcagacca aacctcatag gaatatatac agcagcaccc ttattgtttg agatttttga
     5221 ccagctacca gatgcaccgt ggtttgaaga gccttatgat gatgaagtaa aaataccggt
     5281 gtgtcgcacg agtggataca gggcttcggc gtattgtccg gcagattctg tctttgtacc
     5341 ttcggcagag attcactcgg ctgtgtgtag ctatcatgag cagatataca ccgacctgcg
     5401 tggagaatac agggtgaata tggattgtga aacgggtgcc ggaatggagg ctaagagctg
     5461 gtttgtgtta aaaccaatag aggaatatta cttccgtcag aaaaacagct ggtacgagcc
     5521 attgccgccg tacaaggaag gctgtaaccc aagcataaga ggccgcgaac tcataaacat
     5581 catctacccg agagatgagt cggtggtgtt cttgccccga cagatgagcg gagaaaaagg
     5641 gcaggtaatt ttggaagcag cacaccaacg cagtgacgct gccatcaact ggtttataga
     5701 cgaacaattc atggctacta cacatcaaaa gcacaaaata aacgtgtcgc caacacccgg
     5761 acaacacctg ttggtattgg tggacgataa gggataccgg ctggtgataa ggttcgaggc
     5821 gaaagattcg gagtcaaaga gataaggaaa gtagctaaat aaaaaaaatc ctgcactaat
     5881 atgagggtaa gaggtgcagg atcaatcaac aaacatacta tgagaaaacc gatgtaaaga
     5941 tgagaagctt ttgagctttc gtcaagacag aattcggaaa cggccggaaa aagacaggaa
     6001 cggtaagata catttaggaa acggtaatgt actaaacagc taaaaataac tacaccattc
     6061 aaccggcagt attgaatact atgaaaacca cccaacaaga aatgaacaca taccaaatga
     6121 cgcaagtacg tttttcagct tggctaagac ggcactttta gcacaaatgt tgaatagaat
     6181 tactattgtt cagccttgca ctatcgttca ttcggagccg ttcacccgct ctcttgccaa
     6241 tttgtttgtt atatgccgta tttttatttg tcattaaagt atttttcaat ttcacttagc
     6301 gcattgtcaa gttcgttttc gttactaatt tgctgtccgg ctattgatac attattttct
     6361 atttcctttg cttgaacttt ttgaatagcc gaaattagtt tgtcagccga taaagtttta
     6421 actggtatat gaacacctaa tttcttgcgt tcaactattt taccccaagt tggttggtct
     6481 gtataaaacg aaacaatgat tacaggtaaa ttgtgcctta acattgtagc caaagttcct
     6541 gctccaccgt gaaaaatacc aattttacat ttcggtaaaa tagtttcgtg atttacatat
     6601 tttgtaacga atagattttt atgttcgggt aaattgtcaa actgtgacca acctttgcaa
     6661 aacaaaatgc gttcattggt ctgtactaaa atttctgtca atattttaat gaatttttca
     6721 gtattaccaa ctccattact tccaaaacct atataaattg gcttgtcgcc ttgatctatc
     6781 cattctgtaa gttctgttgg tgttttgtcc aaaaaatgtt catctctttt atgttttgga
     6841 atgtatataa atcccgtgat tttatgatgg ctttcccaat cttttggttg tggaattaac
     6901 gattgactaa tgcaatatag gtctaatatt ttttgtttgt caagataagt aactaaattt
     6961 tcttttaaaa taggtaaacc aagttcttgt ctgtattcgt tggtatcttg cttaataaac
     7021 ttccaaaaaa aactttgtgc aattttaaag gtcagtttgt tgtaccaagg aaaattgaaa
     7081 aaatcaaagt cgcctaatgg aaattctatt gtaggtacaa ctggtggcat aaaataagtc
     7141 aatgctactt tcttattttg tttttcagca atggcacttc caattggtag cgtcatcgag
     7201 ttggcaataa tgaaatcaac ttttgaaata gcttcgaagt aactttttct caatggttct
     7261 ctgttgtcgt gaagcacttt gaaataatat ctcatcaact ttatagcgtt ttcagtttgt
     7321 aaaatgcttt gtccttctgt tgagttcatc attttttcag catttcccca aagaggttga
     7381 aatggcacac caaaaccttc tacaaatgct ttaaagtcat ctgttgctga aattgttacc
     7441 ttgtgtcctt tgtcaacaag tccaagtgct aatgcaacat atggttgtaa gtcgccacga
     7501 gttccgtatg tgaaaattcc tatattcatg acacaaagaa atcaataata ctacactttc
     7561 tttttagcaa aagctaaaaa atcaatattg atttctaatt ctacttaatg ttacctgatt
     7621 catcccaagg tatgaggcta tatatcctaa tggaactcta ttaattaaat tgggatattt
     7681 atatacgaac tcatcatatc gttcttttgc agaaaataat tgttgagtat caattttatg
     7741 ttgtaggaat aagattgtat taattgcaat taaacggcct aatctctcga tgtcgtgatg
     7801 tttttgataa agtttttcca aatcattaaa tgaaacacta actaactctg tatcttctaa
     7861 tgtttctata ctgtggcgag tatttttaat aggaaaattt ctaaccgttg caccaataat
     7921 catattttct attccaaacc agtcggttat ttctttgcca tcattataaa aaaaggctct
     7981 aactatacct ttttttatga aatataattt tttggaagca gagagttctt gaacgaaaac
     8041 ttgcttttta ggtatttttt caactgataa atattcattg atgtagtgaa tagctgagtt
     8101 acttaatttt gaaatgctag ttaaatattc aataaaaata ttcattagtt tgctgcgttt
     8161 ttgtaatatg gcacagaacg tcagtctgtg aaaaaaatat ttcaaaaatt ttaccaaaaa
     8221 tacaaaaaca t
//
LOCUS       DAOQQY010000105         8162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3436_length_8162_cov_5.332799, whole genome shotgun sequence.
ACCESSION   DAOQQY010000105 DAOQQY010000000
VERSION     DAOQQY010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3436_length_8162_cov_5.332799"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..426)
                     /locus_tag="PLE23_06445"
     CDS             complement(<1..426)
                     /locus_tag="PLE23_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82937.1"
                     /translation="MNTFQYIIGYQFDNHSIASEIDQKISRVRFTFDHIHTGNALLNQ
                     SIGERILQHSAPFLLIITDNFLKDENCIYKLYELLVTAEKQNRNFILILAKAWVEDPI
                     SGAIRESETKLTSVGDILYYINYWQDKYLRLRKKHIGEET"
     gene            complement(470..1756)
                     /locus_tag="PLE23_06450"
     CDS             complement(470..1756)
                     /locus_tag="PLE23_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMX82938.1"
                     /translation="MIATSCFEFSGKYSKLPPGYWRGELSLSPKNITPNPEAKPLPEK
                     VGLKFDEVMEGQLPFVFEVKYTNDSLFYIEIINGEERIKVTDIQYGRDRRTAKDTILI
                     NFPEYDSYIHAVCESGVMEGEFVIKSKDNYRIPFTAKHGTNIRFTELKKTPAMDISGA
                     WQTTFDQNTDKPWDAIGVFKQNGNQLTGTFKTETGDFRYLEGTVQDKKLYLSCFDGAH
                     AYLFEAQIEPDSTIQGSFRSGLSEPSLWSAKRNPDFKLKDADSITKTKPGKETVHFNL
                     PNPEGKNISPDNKEYQGKVKIIQVMGSWCPNCKDETKFLTDFLSKADTSKVAVIALSF
                     ERYKEVDKANEMLIKYKKALNVPYEIVLGGNSNKTEAGQKLDFLDELRAYPTMLILDK
                     TNKIRKIHTGFSGPATDEFNKFRDEFGTFIEILLKE"
     gene            complement(1939..2472)
                     /locus_tag="PLE23_06455"
     CDS             complement(1939..2472)
                     /locus_tag="PLE23_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82939.1"
                     /translation="MSTAILGVGSRVNHPAYGVGVVTKLHLVAYDVVFVQHGVKTVGK
                     EYDKWEIIDHIPAEENITYTEAEKSLIRILKSWSDISEVVPLGDRWDGGTMVLNPGEA
                     NLAAKEIPIETFFHKIVMLRDRLRVMEQKINAHSKMSDEEKIEMQQYITRIYGSLTTF
                     NVLFKKKEHQFVGEKSK"
     gene            2596..3720
                     /gene="mnmA"
                     /locus_tag="PLE23_06460"
     CDS             2596..3720
                     /gene="mnmA"
                     /locus_tag="PLE23_06460"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMX82940.1"
                     /translation="MSKKGKILVAMSGGIDSTVAAIMLHEQGYEVIGITMKTWDYASS
                     GGSKKETGCCSLDSINDAREVSVRMGFHHFIVDIREEFGDFVINNFVDEYLAGRTPNP
                     CVLCNTHIKWSALLKRADALDCEFIATGHYAMVNELNHRKFITKSKDMAKDQSYVLWG
                     LTQECLQRSIFPIGEMTKPEVRQLAYNMGYESLSKKAESYEICFVPDNDYRGFLKRRV
                     PELEHNLVGGNFVDPEGNILGKHSGYPFYTIGQRKGLGMAFGSPKFVVEIIPHTNTVV
                     LGDINDLVRNGMQVSKLNLMKYEHLPQNLETVTQIRYNDAGSLSVLNMNGTDTVDVEF
                     LSNVKGVAPGQSAVFYEGDDVVGGGIIMSSSLSELMNNTN"
     gene            3723..4484
                     /locus_tag="PLE23_06465"
     CDS             3723..4484
                     /locus_tag="PLE23_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82941.1"
                     /translation="MNSKTFLVVGLLVFLFGACKKDSSTLPDIETAKAYLPLQIGSEW
                     IYQTDSLIFSKTINSTKIDTSSYLIRYVMVDTFTNSAGELVFITDRYEKKTTDNNWNI
                     KAVSGSFFSQNRFLITEGNNTFIKFSLPVAKGQRWDALALTNKEQTIEVGGEQIEIFK
                     GWESRTVFADKEFDNGIQTYPECFKVELADVESLVELRNGYEVYAKNTGLVYSELRIL
                     DTQCGGNPAACDGIPWEQKAEKGFIVKQSLISYKK"
     gene            4489..5004
                     /gene="rimM"
                     /locus_tag="PLE23_06470"
     CDS             4489..5004
                     /gene="rimM"
                     /locus_tag="PLE23_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HMX82942.1"
                     /translation="MIKIGHTKKTHGLRGGVKATIEEKFYEDFDAAEFLFIEINGKQV
                     PYFIEEVTGGKELIIKFEDIDDIDAARLITSKELFLKEDQIIPAEQRKYSQIGNEYNE
                     TQGFAMISTEGKYIGTISEVMEMPGQIMALVQMDENEEKLIPLNEYFVVEINEAEKQV
                     VIDLPDGLLEL"
     gene            complement(5061..6602)
                     /locus_tag="PLE23_06475"
     CDS             complement(5061..6602)
                     /locus_tag="PLE23_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82943.1"
                     /translation="MKRFFTLLLLLTVMFMANKSEAQKRYFDEIFDKVSVSTHTYGVN
                     ATILYVQVFNEAVPVPLDMDVYQPVGDTETNRPLVLVFHTGNFLPHPQNGSPNGTRKD
                     SAVVDVCTKLAKKGYVVASCDYRLGWAPTATDKTTRVFTILNAVYRGVQDARTAIRFF
                     KRDVNENANSFGIDPSKIVLWGIGTGGYITLNSAALDNYLKIPTASNGKFVTVIGGQT
                     VPMVIPAVNGDIYGTSVGVVPPGYPGFPAGDTLNYPNHLGYDSDFALCVNMGGALADT
                     AWIDPGQVPLIAFHCPYDPNAPYKQGIVKVPVNPPLDVVEVQGSYLATKLSEQYGNNA
                     SFSSVDFKDPVSTVANTRNDGISSLYPIYGTGGAFDSSPWDFWASDNPNNANGLATNP
                     NMSPEKARSYIDSILTFYAYRACYALDLDCKSNSTKDQLSDLQVGLKFGPSPAANQVS
                     FTTNAEYPVKDVYVYDVQGRLVKAKTNINSNNYVLERFSLPSGNYLVNLIFKEGIVTK
                     QITFK"
     gene            complement(6650..>8162)
                     /locus_tag="PLE23_06480"
     CDS             complement(6650..>8162)
                     /locus_tag="PLE23_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX82944.1"
                     /translation="LQNRAKSDEFWSNDYSHYWKFNGISKKMIKLGYPELKFNPVTGT
                     FDPFNFDAANQWLVDNRDSLAYWHNLARTFADGIGSNPGSEISPFYEVGTARFDSLFN
                     EIVSTKSNKKGIGSRFFDKSALYHAHAEYKFEPIFTDYIVVGANARYYKPNSEGTIFY
                     DTAGVSISNYEFGAYTGIQKKFFGGRLITNAAIRLDKNQNFDFIATPAASIVYSPAEN
                     NYLRASFSSAIRNPTLTDQYLYLNVGRAILGGNLHGVDSLVTVESFISYLNTQRYDSL
                     DFFRINPIQPEKVKTFEIGYRTTLFDKMYIDAGYYYSIYDDFLGYKIGVSAKWQLHDP
                     LSRPKEVQAYRYAANSDNTVKTQGFSLGLNYYFGKYYMFTGNYSWNKLIKTFADDPII
                     PAYNTPEHKFNIGVTARDMSLKLGSLRINDLGFAVNYKWVQGFTFEGSPQFTGFIPSY
                     GLVDVQINHKIQKINTSFKLGASNLLNNLHYETYGGPLIGRLAYFSILYDFRK"
BASE COUNT         2394 a         1525 c         1712 g         2531 t
ORIGIN      
        1 tgtttcttca cctatatgtt ttttgcgtaa tcttaagtat ttatcttgcc agtaatttat
       61 gtaataaagg atatctccaa ctgaagtcag tttagtttcg gactcacgta tggcacccga
      121 tatgggatct tctacccatg cttttgccag aattaaaata aaattgcggt tttgtttttc
      181 ggctgttacg agtagttcgt acaatttgta aatacaattt tcatctttca gaaaattatc
      241 agttattatg agtaaaaaag gagcagaatg ttggagaatt ctttcaccaa tgctttgatt
      301 caggagtgca ttgcctgtat gtatatggtc aaatgtgaat ctgacccgac taattttttg
      361 gtcgatttca gatgcaatgc tatgattatc aaactgataa ccaataatat attggaatgt
      421 gttcatgggt ttctcttgct gttgtcttgc tttggtgttt tggttaaatt tattctttta
      481 ataatatctc aatgaacgta ccaaattcgt cgcggaactt gttaaattca tcagtagcag
      541 gtccggaaaa gccagtatgt atctttctta ttttgtttgt tttgtcgagt attaacattg
      601 ttgggtaagc acggagttca tccagaaagt ctaatttttg tcctgcttct gttttgttgc
      661 tattgccacc taacactatt tcatacggta catttaatgc ctttttgtat ttgattagca
      721 tttcattagc tttgtcaact tctttgtaac gttcgaacga caaggcaatg acggctacct
      781 tcgaagtgtc ggctttgctt agaaaatcag taaggaattt tgtttcatct ttacagttcg
      841 gacaccagga acccataacc tgtattattt ttaccttgcc ttggtattct ttgttatcag
      901 gagaaatatt tttgccttca gggtttggca ggttaaaatg tactgtttct ttaccaggtt
      961 ttgtctttgt gatggagtca gcatctttaa gtttaaaatc cggattgcgc ttagcagacc
     1021 atagtgatgg ttcggacaga cctgatcgga atgatccttg gatggtgctg tcgggttcta
     1081 tttgtgcttc gaaaagataa gcatgtgctc cgtcaaaaca ggagaggtaa agctttttgt
     1141 cttgtacagt tccttcaaga tacctaaagt cacctgtttc ggttttaaac gtgccggtca
     1201 gctgatttcc attttgttta aatactccaa tggcatccca aggcttatcc gtattttggt
     1261 cgaaggtcgt ttgccaggcg cctgaaatat ccatagccgg tgtttttttg agctctgtaa
     1321 aacggatgtt tgtcccatgt ttggctgtaa atggaatccg atagttatct ttacttttta
     1381 tgacaaattc accttccatg acaccgcttt cacacacagc atggatgtac gaatcatact
     1441 ccggaaaatt gataaggatg gtatctttag ctgtacgacg gtctcttcca tactgtatgt
     1501 ctgtcacctt gattctttct tcaccattta ttatttcaat gtaaaataaa gagtcattag
     1561 tatatttcac ttcaaacaca aaaggcaact gtccttccat tacctcatca aacttaagtc
     1621 cgaccttctc aggcaaaggt ttagcttccg gattcggtgt aatgtttttt ggcgaaagcg
     1681 acaactcacc acgccagtag cctggaggca atttggaata tttgccacta aattcaaagc
     1741 aagaagttgc tatcattaca aaaaatataa agatggttga tatcttaaaa ttcattgagc
     1801 taattaaata taatttttgg gaaaaaatga atgcacaaag gtacaacttt ttagtgattg
     1861 tatggttcaa gagcctggaa ataaaaagcc ccgaaactaa agttccgggg cttgtaatca
     1921 tattttattg accaattgtt atttagattt ttcacctaca aactggtgtt cctttttttt
     1981 gaacagcacg ttgaatgttg tcagactgcc gtatatccta gtgatgtact gttgcatttc
     2041 tattttttct tcgtcagaca tttttgaatg agcatttatt ttttgttcca taaccctaag
     2101 cctatcgcgg agcattacta tcttatggaa gaatgtttcg atgggtattt ctttagcggc
     2161 cagatttgct tcaccggggt taagcaccat ggtaccacca tcccacctgt cgccaagtgg
     2221 tacaacttca ctaatatcag accaagattt caaaatcctg attaaagatt tttctgcttc
     2281 tgtatatgtg atgttttctt cagcgggaat atggtcaatt atttcccatt tgtcgtattc
     2341 tttgccaact gttttcacgc catgctgaac aaaaaccaca tcataagcca ccagatggag
     2401 cttcgtaacc actcctacac cataagccgg atgattaacc ctgcttccta ctcccaaaat
     2461 agctgtactc atattcattt ttttgataat atgtacgaag gaatgttgaa aatgttaaac
     2521 cattttgatt tttcgttctt taagttgatt agaatatcga tttcaaaatt aaaaaaggcg
     2581 taattttgca agacaatgag taaaaaagga aaaatccttg tagccatgag tggcggtatt
     2641 gatagtacag ttgcagctat tatgttgcat gagcaaggtt atgaagtaat cggtataacc
     2701 atgaagacat gggattatgc atcatcgggt ggcagtaaaa aggaaaccgg atgttgtagc
     2761 cttgactcta tcaacgatgc tcgcgaggtt tctgtacgta tggggtttca tcactttata
     2821 gttgacatac gcgaagagtt tggcgatttt gtaatcaata attttgttga cgaatatctg
     2881 gctggccgta cacctaatcc ttgtgttttg tgcaacacac acatcaaatg gtcggcgctg
     2941 ctcaagcggg ctgatgcact tgactgcgag tttattgcca ccgggcatta tgcgatggtt
     3001 aatgaactaa accatcgcaa gttcattacc aagagtaagg atatggccaa ggaccaatct
     3061 tatgtacttt gggggctcac acaggaatgt cttcaacgaa gcatttttcc catcggagaa
     3121 atgaccaagc ccgaggtaag acagctggca tataatatgg gttatgaatc gctttcaaag
     3181 aaagcagaaa gttatgaaat atgcttcgtg ccggacaacg attatcgcgg ttttttgaag
     3241 agacgtgtac cagagctcga acataacctt gtcggtggca attttgtaga tccggaaggc
     3301 aatattcttg gcaagcatag tgggtatccg ttttatacta tagggcagag gaaaggcttg
     3361 ggtatggcat tcggcagccc caaatttgta gtggaaatta ttccacatac caataccgtg
     3421 gtactgggtg atataaacga ccttgttcgc aatggcatgc aggtaagcaa attgaaccta
     3481 atgaagtacg aacatttgcc acaaaacctt gaaaccgtta cacagattcg gtacaatgat
     3541 gccggttcct tgagtgtact gaatatgaat ggtaccgata ctgtagatgt ggagtttctc
     3601 tcaaacgtta aaggtgtcgc tcccggtcag tcggctgtgt tttatgaagg tgacgatgtg
     3661 gtaggcggtg gaatcattat gtcctcatcg ctgagtgaat tgatgaataa cacaaattaa
     3721 tcatgaatag taaaacattt ttggttgttg gtttattggt atttttgttt ggtgcatgca
     3781 aaaaagattc ttcaaccttg cctgatatcg aaacagcaaa ggcatacctg ccactacaaa
     3841 ttggttcgga gtggatttat caaacggaca gcctcatttt tagcaaaaca ataaacagta
     3901 ctaagattga cacctcaagt tatcttataa gatatgttat ggttgacaca tttaccaatt
     3961 cggcaggtga gcttgttttt attacggaca gatacgagaa gaaaacaacc gataacaact
     4021 ggaatatcaa agctgtgtca ggtagctttt ttagccaaaa cagattcctc attaccgaag
     4081 gcaataacac ttttattaaa ttctcgctac ccgtagcaaa aggccaacga tgggacgcat
     4141 tggcattgac taacaaagaa cagactattg aagtaggtgg tgagcaaatt gaaatattca
     4201 agggctggga gtcaaggaca gtatttgccg acaaggaatt tgataatgga attcagacat
     4261 atcccgagtg ttttaaggtt gaacttgcag atgtagagtc tctggtagaa ctgcgaaacg
     4321 gatatgaagt ttatgctaag aataccggac ttgtatattc cgaactaagg atactcgaca
     4381 cacagtgtgg cggcaatccg gcagcttgtg acggcattcc ttgggagcaa aaagcagaaa
     4441 aaggatttat tgttaagcaa agcttgattt cttataaaaa ataattggat gataaagata
     4501 gggcatacca aaaaaacaca tggactgaga ggaggtgtga aagcgaccat agaagaaaaa
     4561 ttctacgaag attttgatgc tgccgagttt ctctttattg aaataaacgg aaaacaggtg
     4621 ccttatttta tagaagaggt gacaggtgga aaagagttga ttatcaaatt tgaagatatt
     4681 gacgacatcg atgctgcccg acttatcacc agcaaagagc tttttctaaa agaagaccag
     4741 atcataccgg ccgagcagcg taagtatagc caaataggca atgaatataa cgaaacgcaa
     4801 ggatttgcaa tgatttcaac tgaaggtaaa tatattggta ccatcagtga agtaatggaa
     4861 atgcccggac aaatcatggc attagtccaa atggatgaaa atgaagagaa gctgattcca
     4921 ctgaatgagt attttgtcgt cgagataaac gaggcagaaa agcaagttgt tattgatttg
     4981 ccggatggcc tgcttgagtt ataaaaaaac gtctgcaaaa atcaatctgc agacgttctt
     5041 caatataaac ggacaaatta ctatttgaat gtaatttgct tcgttacgat tccttccttg
     5101 aatatcagat tgacaagata gttgcctgaa ggaagactaa atctttcaag tacataattg
     5161 ttactgttga tatttgtttt tgcctttacc aacctgcctt gtacatcata aacatataca
     5221 tcttttacag ggtattcggc attggtagtg aaacttacct ggttagcagc ggggctaggg
     5281 ccgaatttca aaccaacctg taggtcactc aattgatctt tggtgctatt tgatttacag
     5341 tcaagatcaa gtgcataaca tgctctgtaa gcatagaagg taagaatcga atcaatatac
     5401 gaacgtgcct tctctggaga catattaggg ttagtcgcaa gaccattggc gttgttagga
     5461 ttgtcagaag cccagaagtc ccaaggagaa ctatcaaatg caccgccggt tccgtagatt
     5521 gggtacaagc ttgatatgcc gtcatttctg gtattagcta cggtagatac agggtctttg
     5581 aaatcaaccg aagagaatga agcattattg ccatattgct cagaaagttt agttgcaaga
     5641 tatgatccct gtacttctac cacatcaaga ggtggattaa ccggtacctt tacgatacct
     5701 tgcttataag gtgcattcgg gtcgtatggg caatggaatg caataagagg cacttgtccc
     5761 ggatctatcc aggctgtatc tgccaaagca ccgcccatgt taacgcagag tgcgaagtca
     5821 gaatcataac caaggtggtt agggtagttt aaggtatcac ctgcagggaa accagggtaa
     5881 cccggcggaa ctacacctac agaagttcca taaatatcgc cattaaccgc cggaataacc
     5941 attggaacgg tctgtccgcc gataactgtt acaaacttac cgttactggc agtaggtatc
     6001 ttcaggtaat tgtcaagtgc tgcggagtta agtgtgatat aacctcctgt gccgatgccc
     6061 caaagaacta ttttgctcgg atctatacca aatgagttgg cattttcatt aacatctcgc
     6121 ttgaaaaaac gaattgctgt tcttgcatcc tgaacgccac ggtaaacggc attgagtata
     6181 gtgaatactc tggtagtttt gtcagtagcg gttggagccc atccaagacg atagtcgcat
     6241 gatgctacta cataaccttt tttggctaac ttagtacaaa catctactac tgccgaatct
     6301 tttcttgtac cgttagggct tccgttttgt ggatgtggta aaaaattgcc tgtgtggaat
     6361 acaagcacaa gcggtctgtt ggtttcggta tcacctaccg gctgataaac atccatgtcg
     6421 agtggtacag gaacagcctc gttgaaaacc tgaacataaa gaatagtagc attcaccccg
     6481 taggtatgtg tgcttacgct tactttatcg aatatttcat cgaagtagcg cttttgtgct
     6541 tctgacttat tagccataaa catgactgtc aggagtaaaa gtagtgtaaa aaatcttttc
     6601 attgatgcat aaaatttaat gattgacaaa tataattaat attataaaat tacttcctga
     6661 aatcatacaa aattgaaaaa tatgccaacc ttccgattaa tggtccgccg tatgtttcat
     6721 agtgcaaatt gttaagcaaa ttggatgctc cgagtttaaa gcttgtgttg atcttctgta
     6781 ttttgtgatt tatctgtaca tctaccaatc cgtaagaagg tataaagcca gtaaactgtg
     6841 gcgaaccttc aaacgtgaat ccttgtaccc atttgtagtt gactgcaaaa ccaagatcat
     6901 ttattcggag tgatccaagc tttaaggaca tgtcgcgtgc tgtgactcct atgttaaatt
     6961 tgtgttcggg tgtgttgtaa gccggaatga tagggtcgtc agcaaaggtt tttatcagtt
     7021 tgttccacga atagttgccc gtaaacatat aatatttacc aaaataataa ttaagcccaa
     7081 gcgaaaatcc ctgtgttttg accgtattgt ctgagttggc tgcatatctg tatgcctgta
     7141 cttcttttgg gcgagagagt ggatcatgta gctgccattt ggcgcttacg ccaattttat
     7201 aaccaagaaa atcatcgtag atactgtaat aataacctgc gtcaatgtac attttgtcaa
     7261 aaagtgtagt tctgtatcct atttcgaatg tcttaacttt ctccggctgt atagggttga
     7321 ttctgaaaaa atccagtgaa tcgtagcgtt gtgtattgag atagctaata aaagattcta
     7381 ccgtcaccaa gctatccacg ccatgaaggt tgccaccaag tatagcgcga cctacattca
     7441 gataaagata ctggtcggta agtgtcggat ttctgatagc cgaagagaat gaagctctca
     7501 gatagttgtt ttcggctggt gaatatacga tagaagctgc cggtgtggcg atgaagtcaa
     7561 aattctgatt tttatcgagt cttattgcag cattggttat cagacgccca ccaaagaatt
     7621 ttttctggat acctgtatat gccccgaact cgtaattgga tatggacact ccggcggtat
     7681 cgtagaatat agttccttca ctatttggtt tgtagtaacg agcattggcc cctacaacaa
     7741 tgtaatcagt aaaaattggc tcaaatttat attctgcatg cgcatggtac agtgccgatt
     7801 tgtcaaaaaa cctggagcca ataccttttt tgttggattt tgtactcact atttcattaa
     7861 acaatgaatc aaaccttgcc gtacctacct catagaaagg tgaaatctcc gaacccggat
     7921 tgctaccaat accatcggca aatgtacgtg caagattgtg ccaatatgcc agtgagtcgc
     7981 ggttgtcaac aagccactgg ttggccgcat caaagttaaa cggatcaaaa gttccggtta
     8041 caggattaaa tttcaactca ggataaccaa gtttaatcat tttcttggag ataccgttga
     8101 atttccagta atggctgtag tcatttgacc agaattcatc cgattttgct ctgttttgta
     8161 aa
//
LOCUS       DAOQQY010000106         8144 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3446_length_8144_cov_6.115589, whole genome shotgun sequence.
ACCESSION   DAOQQY010000106 DAOQQY010000000
VERSION     DAOQQY010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8144)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8144)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8144
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3446_length_8144_cov_6.115589"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLE23_06485"
     CDS             <1..297
                     /locus_tag="PLE23_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HMX82945.1"
                     /translation="IAKYALSALNVDEHGLDEMDNRILATIIEKFKGGPVGLTTIATA
                     VGEEAGTIEEVYEPFLIMEGYIQRTPRGREATEKAYKHKGMKSPYTKGTLFDTD"
     gene            complement(294..608)
                     /locus_tag="PLE23_06490"
     CDS             complement(294..608)
                     /locus_tag="PLE23_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HMX82946.1"
                     /translation="MSENQNTNQLNIELPEDVADGIYSNLAIISHSNAEFVVDFIRMM
                     PNVPKAKVKTRIILTPQHAKRLLKALNENIKKYETQFGVIDDNDHGFMPGMNFNTPPA
                     QA"
     gene            complement(669..1403)
                     /locus_tag="PLE23_06495"
     CDS             complement(669..1403)
                     /locus_tag="PLE23_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMX82947.1"
                     /translation="MTDIKRVREELYLDGPRSRIEEFKFLISVMWNFLKGFRKLHFSG
                     PCITVFGSARFKEDHKYYQVAREIGASIAKLGFTTLTGGGPGIMEAANRGAFEAGGKS
                     IGCNIILPFEQLPNPYTQTNFSVRYFFVRKVLLLKYSYGFVVLPGGFGTMDEFFETLT
                     LIQTGMIRDFPIVLIGKEYFKPMTHMIEHMIQNKTISPEDLDLVLITDDLKEAMNHIR
                     QYIDQNYIVRRQKPRWWLLEEVKPVN"
     gene            1663..4029
                     /locus_tag="PLE23_06500"
     CDS             1663..4029
                     /locus_tag="PLE23_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMX82948.1"
                     /translation="MKYLKLIIFILLCQLPLFSQSALLFHRVEIPLDSTNIERIATFG
                     IDISEGIHLPGNKFSIELSEEEISILKSNAVPYAIRIYNVSEYYRTRKHSDAPTVRDA
                     AVCNPEPTIDPPKNFKKGSMIGYYTYQEFLDVLTEMHTKYPQLISAYKPIGNYKTHNN
                     NPLYWLRISDNPDVEEDEPKVLYTALHHAREPLSLTQLVYFMWYVLEHYETDAEIKAL
                     VDNTEMYFIPMINPDGYRYNEFTDPMGGGMWRKNLKPNTNGGMGVDLNRNYGKFWGYD
                     NLGSSPSSVSQIYRGLSAFSEVETQAVRSFCLEHDFKFALNHHASGNLLLYPWGHNAS
                     NCPDSLTFRRVADRMVDKNKFTAGTSLETVGYYANGTSDDWMYGGDEKTSPTIALTAE
                     VGEFYDGFWPFEDRILPLSQSCLEMNLQILRSAHNNLALFYKEIPAMSGEDTILTLDA
                     IQTGISPAPVTVTISEPTDRMIFTQPVQVISLNTNDIQKIKFDFAWGNVKNGDQITVY
                     FNWTDGYTTHQDSVNIGFYDVKETLLTDEFENLDNWTASTPWGITTSDAYSGGTCVTD
                     SPTGTYSADEFSYLALKQALVTSGQYQKVYLRYYAKWAFQPNFDYAAVSIEDNTAGTT
                     EYLCGYNMTKGTLEQAAENSVYEGNALKWEEEVIDISQYNNSVFNLIFELGADETDQR
                     DGFYIDKFEVVGYREKTTSTGAMSSGSLRIYPNPVQDYFTVNGVADYDRVEIFNLLGN
                     SVFYQNNIDHSIAEKYLGYRCDLDSGMYLVHFSKNGMLISSQKLLVVK"
     gene            complement(4062..5111)
                     /locus_tag="PLE23_06505"
     CDS             complement(4062..5111)
                     /locus_tag="PLE23_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HMX82949.1"
                     /translation="MTLLIIIIVLALVFDYINGFHDAANSIATIVSTKVLTPVQAVIW
                     AAFFNFLAFWLFELNIADTISKTVNTGAIDLWVVFAGLIAAIAWNLLTWYFGIPSSSS
                     HTLIGGFAGAAIAHAGIKIVNTKIIIKVAAFIFLAPIIGMVISFIFTTILLWLFRRTH
                     PSRVELVLKRGQLLSSALFSLGHGGNDAQKVMGLIGAAVLVYLNTHNLTPDDVPQWLH
                     VTMVDVNGHQQLHLPNWVPLACYTAIAIGTLSGGWRIVKTMGSKITKITPIEGVAAEA
                     AGAAMLFIADMLKVPVSTTHTITGSIIGVGLTKRTSAVRWGVTINLLWAWILTIPVSA
                     LVGGLTYTVFSWMFH"
     gene            complement(5137..5784)
                     /locus_tag="PLE23_06510"
     CDS             complement(5137..5784)
                     /locus_tag="PLE23_06510"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HMX82950.1"
                     /translation="MKIDDILQFFLPKNEKFYPLFEEAAQNLVNISKKLILMLETPGK
                     QDRSRIIKEIEDLEHVGDGLAHKIVYELNTNFITPFEREDIHQMMVAIDDVVDYIHGS
                     AKRIDLYNIEEFPKSFMDLAIINLKAAEQLQIAVKNLRNLKNRDDIKKACILINEYEN
                     QADDIFDNEVARLFVETKDAVEIIKIKEVLSAIETATDKCEDASMIIDGILVKNA"
     gene            complement(5901..7574)
                     /locus_tag="PLE23_06515"
     CDS             complement(5901..7574)
                     /locus_tag="PLE23_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMX82951.1"
                     /translation="MSWEALFRRKSITQVVQESANREGGHQLHRILGVRDLTAFGIAA
                     IIGAGIFSTIGRASFAGGPAVSLLFVGVAIACVFTALCYAQFASMVPVSGSAYTYAYV
                     AFGELFAWIIGWALVLEYAVSNMVVAISWSEYFTSMLTGFGINMPGWLTIDPGTARHA
                     YQSLELANQNGTVGEMSEQALRTAQIYAEAPDFGSFKVIFDLPAGLITVLVTWLVFRG
                     IRESRNTSNILVVLKLAVIILVIIAGAFFIKPENWSPFAPNGVSGVLSGVAAVFFAFI
                     GFDSISTTAEECKNPQRDLPRAMIYCLVICTVLYIAITLVLTGMVNYTELRVDDPLAY
                     VFSKINMDFMAGIISVSAVIAITSALLAFQLGQPRIWMTMSRDGLLWKKFSAIHPKYK
                     TPWFSTLVAGLIVAIPSLFMDMQFFVDLTSVGTFFAFILVCGGVLYLDEHGHSKEAKF
                     KVPYINGKYIIGVMMIISLIVFRNDLWIHIQEKPLLIAFWLTWLFLSVFGFRNNYSLL
                     PVLGILTNLYLMTELGVTNWFMFLLWLVIGLIIYYFYGRKHSKLRAAHS"
     gene            7714..>8144
                     /locus_tag="PLE23_06520"
     CDS             7714..>8144
                     /locus_tag="PLE23_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMX82952.1"
                     /translation="MTNRIDSQSGTTPTPKLMVRLILEQDDHVVALKRRKKIGGGYGL
                     VGGTVCKNESLKDALVRETYEEIGVMLDKDMLQLVHVMQRFKKLTQTVSMVFFFRYHA
                     KPQTLSVREANKFKEVVWLPLNNLPQNMSASLVEGMTSAVRG"
BASE COUNT         2413 a         1694 c         1695 g         2342 t
ORIGIN      
        1 atagcgaagt acgccctttc tgccttaaat gttgacgaac acggtctgga tgaaatggat
       61 aataggatac tagccactat catcgaaaaa ttcaagggtg gtccggttgg ccttacaacc
      121 attgccacgg cagtaggtga agaagcaggt accatcgaag aagtttatga accctttctc
      181 atcatggaag gctatataca acgcacacca cgcggacgtg aggcaactga gaaggcgtac
      241 aaacacaagg gtatgaaatc gccttacaca aaaggtacat tatttgatac agactaagct
      301 tgtgccggcg gtgtattgaa gttcataccg ggcatgaaac cgtggtcatt atcatctatt
      361 acgccaaact gtgtttcgta cttcttgatg ttctcgttta atgctttcaa caaccttttt
      421 gcatgttgcg gtgtaaggat aattcttgtc ttaaccttag ctttgggcac gttgggcatc
      481 atacgtataa aatcaaccac gaactcggca tttgaatgcg aaatgattgc aagattggag
      541 tatattccgt ctgctacgtc ttcaggcagc tcgatgttca gttggttcgt attttgattt
      601 tcgctcatga ttattttttt tatagtatta gtaacgtatt tattgatcta aaagttttat
      661 ccattttttc aatttaccgg ttttacttct tccagtaacc accaccgtgg tttttgtctg
      721 cgtacgatgt agttttggtc aatgtattgt ctgatgtggt tcattgcctc ctttaggtca
      781 tcagttatta gtacaaggtc gaggtcttcg ggcgaaatgg ttttattttg tatcatgtgt
      841 tcaatcatgt gggtcatcgg cttgaaatat tcttttccga tcagcacaat cgggaaatct
      901 cggatcattc ctgtctgtat gagtgtgagt gtttcaaaga attcatccat agtaccgaag
      961 cctcccggca atactacaaa accgtatgaa tattttagca acaacacttt tctgacgaaa
     1021 aaataccgta ccgaaaaatt ggtttgagtg taagggttgg gtaattgttc gaaagggaga
     1081 atgatattgc accctataga tttgccgccg gcttcaaatg ctcctctgtt ggctgcctcc
     1141 atgattcccg gcccgccacc tgtaagtgtc gtaaaaccca gttttgctat ggatgcccct
     1201 atttctcttg ccacctgata gtatttgtga tcttccttga atcttgccga accaaaaaca
     1261 gtaatgcagg gtccgctaaa gtgtaatttc ctgaatcctt ttaaaaaatt ccacattaca
     1321 gatatcaaaa atttaaattc ttctatcctg cttcgtggcc cgtcgaggta taattcttcc
     1381 cttactcgtt ttatgtctgt cattgtacag cttctttata tcaattaaca aagatagtca
     1441 ttttgaaaca cagaatgtct tgaataaatg ttaatgcact taggtttttt gaaatggatt
     1501 tgaagcaata attatttgca gtaaagagcc taatgcgtta tttgggttta acaattggtt
     1561 ttataaacat tcttgcttaa gcttacgtta aattcctgca tttcatctca aatgaatata
     1621 attttgagaa ttatttaaag cctacatttc aatataatct gcatgaaata tctgaaactg
     1681 atcatcttca ttttactttg tcaattacca cttttttcgc agtctgcctt actttttcat
     1741 cgtgtagaga ttccattgga cagtacaaat atcgaaagaa ttgctacatt tggcatcgac
     1801 atttctgaag gtatccactt accgggtaat aaattttcaa tcgagttatc agaagaagaa
     1861 atttcaatat tgaaatcgaa tgctgtacct tatgccatcc gtatatacaa tgtgtcagaa
     1921 tattaccgca cacgcaaaca ttctgatgca cccaccgtac gtgatgcagc tgtctgtaat
     1981 cctgaaccca ctatagatcc acccaaaaac tttaagaaag gatctatgat agggtattat
     2041 acctaccagg aatttctaga tgtgctaacc gaaatgcaca ccaagtaccc tcagctcatt
     2101 tcagcttata agccgatcgg caattacaag acacacaaca ataatcctct gtattggttg
     2161 cgaatatccg acaacccgga tgtggaagaa gacgagccta aggtactcta taccgccctg
     2221 caccacgcac gcgaaccgct tagtctcaca cagcttgttt attttatgtg gtatgtgcta
     2281 gagcattatg agaccgatgc tgaaataaag gccttagtgg acaataccga gatgtatttt
     2341 ataccgatga ttaacccgga cggctaccga tacaacgaat ttaccgaccc catgggtgga
     2401 ggaatgtggc gcaagaacct gaaaccaaac accaatggcg gaatgggtgt agatctgaac
     2461 cgcaattacg gtaaattctg gggctatgat aatttggggt cctcaccttc gtcagtaagt
     2521 cagatctatc gcgggctttc tgcctttagt gaagtagaga cacaggctgt gaggagtttt
     2581 tgtcttgaac atgatttcaa atttgcgctc aaccaccatg catctggcaa tttgttattg
     2641 tacccttggg gccataatgc atccaattgt ccggactctc tcacattcag acgagtggca
     2701 gacaggatgg ttgataaaaa caaatttacc gcaggtacca gtcttgagac ggtaggatat
     2761 tatgccaacg gtacttcaga tgactggatg tacggaggcg atgaaaagac tagtccgacc
     2821 atagctctca ctgcagaagt tggtgaattt tatgacggat tctggccatt tgaagaccgg
     2881 attttgccgt tgtcacagtc ttgtcttgaa atgaacctgc agatactcag atcagcacac
     2941 aacaacctgg ctttgtttta taaagaaatt ccggctatgt caggtgagga taccatattg
     3001 acactcgatg ccatccagac gggcatttca ccagcaccgg ttacggttac gatttcagaa
     3061 ccaactgaca gaatgatatt cacgcagcca gtacaagtca tatcgcttaa tacaaatgat
     3121 attcaaaaaa tcaagtttga ttttgcctgg ggcaatgtca aaaacggtga ccagataaca
     3181 gtttacttca actggaccga tggatacacc actcatcagg attctgtcaa cattgggttt
     3241 tatgatgtta aagaaacact acttacagac gaatttgaga atctcgacaa ttggacagca
     3301 tctacaccat ggggcatcac cacatccgat gcatattcgg ggggcacatg tgttacggat
     3361 agtccgactg gtacatattc agcagacgaa tttagctacc tggctttaaa acaagctttg
     3421 gtaacttccg gccagtatca gaaagtttac ttgaggtatt atgccaaatg ggcattccaa
     3481 cccaactttg attatgcggc agtgtcaata gaagataata ctgctggtac tactgaatat
     3541 ttgtgcggtt acaatatgac caaaggcaca ctcgagcagg cagcagaaaa ttctgtgtat
     3601 gagggcaatg cattgaaatg ggaagaggag gtgatagaca tatctcaata caacaattct
     3661 gtttttaacc tgatattcga actcggggca gacgaaaccg accaacgtga cggattttat
     3721 atcgacaagt ttgaagtagt cgggtacaga gaaaagacca catcaaccgg agcaatgtca
     3781 agtggaagct tgcgtattta tccgaatcct gtgcaggatt attttactgt aaatggggtg
     3841 gcagactacg acagggtaga gatttttaat ctattgggca acagtgtttt ctaccaaaat
     3901 aatatagacc attcaatcgc agagaaatac ttgggttata gatgtgactt agactcgggt
     3961 atgtatctcg ttcattttag caaaaacggc atgctcattt catcacaaaa gctactcgtc
     4021 gtcaaataag tcttcattta gaaacgagag tggatgatac cttaatggaa catccacgaa
     4081 aatacggtat aagtaagacc gcctaccaaa gccgaaacag gtatggttag tatccaagcc
     4141 cagagcagat tgatggttac gccccatcgt actgcagaag ttcgcttggt gagccctaca
     4201 ccaatgatag agcccgtaat ggtgtgtgta gtacttacag gcaccttaag catgtcggca
     4261 ataaagagca tggcagcgcc tgctgcttca gctgcaacgc cttctatagg tgttattttt
     4321 gtaattttcg aacccatggt ttttactatt ctccagccac cacttagcgt gccaatggca
     4381 atagctgtgt agcatgctag cggcacccaa ttgggtaaat gaagctgctg gtgcccgttg
     4441 acatctacca tagtcacatg cagccactgc ggtacatcat ctggtgtcag gttgtgtgtg
     4501 tttagataaa caagtacggc agcacctatc agtcccatta ctttttgagc atcgttgcca
     4561 ccatgcccta agctaaaaag tgccgatgaa agtagctgac cacgcttcaa tacgagttct
     4621 actcttgaag ggtgagtacg tctgaacaac cacagcaata ttgtagtaaa tataaaggag
     4681 ataaccatac caatgatagg tgcaagaaag ataaatgcag ctactttaat aatgattttg
     4741 gtattcacaa tctttatacc ggcatgtgct atggcggccc ctgcaaaccc gcctatgagt
     4801 gtgtgtgacg aacttgaagg aatgccaaaa taccaagtta aaagattcca ggcgatggca
     4861 gcaataagtc cggcaaatac aacccacagg tcaatggcac ccgtattgac agtttttgaa
     4921 atggtatcag caatgttcag ctcaaaaagc cagaatgcaa gaaaattaaa aaacgcagcc
     4981 catattacag cttgtaccgg ggtcaatacc ttagtactta cgatggtagc aatggagttg
     5041 gcagcatcat gaaaaccgtt gatgtagtca aatacgaggg cgagtactat gataatgatt
     5101 agtaatgtca taaatgtttg ttcttggtgg tttggcctac gcgtttttta ctaggattcc
     5161 gtcaataatc atggaggcat cttcgcattt atctgttgct gtttcgatcg ctgagagcac
     5221 ttctttgatt ttaatgattt caacagcgtc cttggtttcg acgaacaaac gtgccacttc
     5281 attatcaaaa atatcatcag cttgattttc atattcgttg atgagaatac aggccttctt
     5341 gatgtcgtcg cggtttttta ggttgcgtag atttttgacg gcaatctgca attgttcagc
     5401 tgcttttagg ttgataatgg cgaggtccat gaaggatttt ggaaactcct caatattgta
     5461 caagtcaatc cttttggcag agccatgaat gtaatcaact acatcgtcaa ttgctaccat
     5521 catttggtga atgtcttcgc gttcgaatgg tgtaatgaag tttgtattga gttcgtacac
     5581 tattttatga gctagtccat cacctacatg ttcgaggtct tctatttctt taataatgcg
     5641 cgatcggtct tgtttgccgg gtgtttcgag catcagtatc agttttttgg agatattgac
     5701 cagattttgt gcagcttctt cgaaaagtgg gtaaaatttt tcgttttttg ggaggaagaa
     5761 ttgtagaatg tcgtcaattt tcatattggc ttttattatg ttgttgataa ttcggtgcaa
     5821 aaatacatat tcgttgtatc acgtttccta attctatatt aagttaatgt taagtttcta
     5881 ttacaattcc tcattccttg ttatgaatgt gccgcccgca gtttactgtg cttacgtcca
     5941 tagaagtaat aaatgataag gcctatgacc agccatagta aaaacataaa ccagttggtg
     6001 acacccaatt ctgtcattag atagagattg gtaagtatgc cgagcactgg tagcagcgaa
     6061 taattattgc ggaatccaaa cacagatagg aaaagccacg ttagccagaa agctataagc
     6121 aaaggttttt cctggatgtg tatccacaaa tcatttctga atacaataag tgaaataatc
     6181 atcatcactc caataatgta tttcccgttg atataaggaa ccttaaattt tgcctccttc
     6241 gagtgcccat gttcgtcaag gtaaagaacg ccaccacata ccaagatgaa tgcgaagaaa
     6301 gtgccaacac tcgttaagtc aacaaaaaac tgcatatcca taaacaagga aggtatagcc
     6361 actatcaagc cggctaccag tgttgaaaac caaggtgttt tgtattttgg atggatagca
     6421 ctgaattttt tccacaacaa tccgtcgcga ctcatcgtca tccatatacg gggctgaccc
     6481 agttgaaatg ctagcaaagc gctcgtaata gctatgacgg cactcaccga aatgatgcct
     6541 gccataaagt ccatgtttat ttttgaaaaa acataagcaa gggggtcatc cacccgtagt
     6601 tcggtatagt tgaccattcc tgttaataca agcgtaatag caatgtataa tacagtacag
     6661 atgaccagac agtatatcat agcacgtggc aagtcgcgtt gtgggttttt gcattcttca
     6721 gctgtagtgg atatggagtc aaaaccaatg aaagcgaaaa atacagcagc tactccactg
     6781 agtacaccgc ttacgccatt gggtgcaaaa ggtgaccagt tttcgggttt aataaaaaat
     6841 gctccagcaa tgatgaccag tattattaca gcaagcttta ataccacaag tatattactt
     6901 gtatttcttg attccctgat gcctctgaac accagccagg taaccagaac agtaataaga
     6961 cctgcaggca gatcaaatat cactttgaat gatccaaagt caggagcttc tgcatagatt
     7021 tgtgcagtac gcaatgcctg ctcagacatt tcgccgacag ttccattttg gttggcaagt
     7081 tcgagacttt ggtaggcatg gcgggctgtt ccggggtcta tagtaagcca ccctggcata
     7141 ttgataccaa agcctgtaag catcgaagta aaatattctg accacgagat agccaccacc
     7201 atattagata ctgcatattc gagaacgagt gcccagccta ttatccaagc aaataattcg
     7261 ccaaatgcta catacgcata agtataggcg ctaccactta ccggcaccat cgaggcaaac
     7321 tgagcataac aaagtgctgt aaaaacacaa gcaatggcta cacctacaaa taagagcgat
     7381 acggccgggc cacctgcaaa actcgctctg ccaatggtac tgaatatgcc ggcaccaatg
     7441 atggccgcaa taccaaatgc agtaaggtcg cgtacgccaa gtatcctatg caactggtgc
     7501 ccgccttctc tgttggccga ttcctgaaca acttgagtaa tagattttct cctgaataat
     7561 gcttcccaac tcattgctga tgattaaatt tgttcaaaaa tactaaaatc tggcgctaag
     7621 aaaactttta tgcaaaataa taatcgaaat tattttaccg gaactttttc gctgtctaaa
     7681 acgtatctca tagactttaa tgttaattga ccaatgacca acagaataga ttctcagtca
     7741 gggacgaccc cgacaccaaa gctgatggtg cgcctgattc tcgaacaaga tgaccatgta
     7801 gtagccctaa agcgcaggaa aaaaatagga ggcggttacg gtctcgtagg cggaactgtc
     7861 tgcaagaatg aatctttaaa agatgccctc gtacgcgaaa catatgaaga aataggcgtt
     7921 atgcttgaca aagacatgtt gcagctggta catgtaatgc agcgctttaa aaaactcacc
     7981 cagactgttt ctatggtttt cttctttaga tatcatgcaa aaccccaaac actgagtgtc
     8041 cgcgaggcaa acaaatttaa agaagtcgtc tggctgcctt tgaacaatct gccacaaaac
     8101 atgtctgctt ctcttgtcga aggaatgaca agtgctgtca gagg
//
LOCUS       DAOQQY010000107         8118 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3469_length_8118_cov_5.975319, whole genome shotgun sequence.
ACCESSION   DAOQQY010000107 DAOQQY010000000
VERSION     DAOQQY010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3469_length_8118_cov_5.975319"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLE23_06525"
     CDS             complement(<1..438)
                     /locus_tag="PLE23_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82953.1"
                     /translation="MNAYRISGILITLALLFYGYRGELIDVNEGAGWDGRLYASYTAH
                     LDRSLEEKAINSYRFQRVLTSVVINKVMKAMDIPFTVPNIVAGFRLFNLLLLMLGLLY
                     YYLISRRLKLSAELEFLGLAALFWCYPVLKMPFFNPIITDAAAF"
     gene            complement(425..1537)
                     /locus_tag="PLE23_06530"
     CDS             complement(425..1537)
                     /locus_tag="PLE23_06530"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMX82954.1"
                     /translation="MKVPFLSFDPIHIPLKEEMMAAMEAVYDSNSFILGEYVNEFESS
                     FADFCQAKYCTGVGNGLEALHIALKCLNIGQGDEVIVPANTYIASALAVSYTGAQVVF
                     VEPDARTYNIDVQKIEEKISPKTKAIMPVHLYGQACEMDVVMEIAKKHNLYVIEDNAQ
                     SQGAAYKGKFTGSWGDINGTSFYPGKNLGAIGDAGAITTNSAEFARLTKIWRNYGSEK
                     KYYNEVQGFNSRLDELQAAFLSVKLRRLQEWTESRQKTAAEYLRQLSSCDFLVLPHTH
                     EHATHAYHLFVITTPYRDKLQQYLSEQGIGTLIHYPVPPHLQKAYKNLNLGNGSFPIA
                     EKLAHECLSLPMFAGMKDEQVSYVCEAIKKFGNECI"
     gene            complement(1547..2947)
                     /locus_tag="PLE23_06535"
     CDS             complement(1547..2947)
                     /locus_tag="PLE23_06535"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX82955.1"
                     /translation="MNPNTEFVPYDESFLHRSSTWLSDPEIKQLTLTPDITPESQQKW
                     FDSLPFKNDYLIWGICIDGIPAGACGLKNISAESAEYWGYIGEKQFWGNGAGSKILDF
                     IFEEAIKRKIPAIGLRVWNGNQRAISLYKKFGFEIIENQTELIVMRKLVEIPMFSIRK
                     YEKMDAATWNEFVRSSRNGTFLHNRNYMDYHADRFEDFSLMVFDTNGKLAGIIPANRR
                     EHVFYTHQGLTFGGIITQTDMTTAGHLRISDLLNHFLRENGFSKVVFKKIPYIYARQP
                     IDEDRYLMFRNHAKITACNISTTLDLSENFSYNRSRKNAVGKSLKQGLELHKNQHWKE
                     FWNILEENLHQKYGTKPVHSLAEILRLHASFPENIHLFTASKDCKCLGGIVVYESDTV
                     AHAQYISINEAGKTHCALDFILDHLIRTEFSHKKYFDLGSSTEDNGYYLNESLIFQKE
                     GFGGRGVCYETFEYYL"
     gene            complement(2952..3371)
                     /locus_tag="PLE23_06540"
     CDS             complement(2952..3371)
                     /locus_tag="PLE23_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HMX82956.1"
                     /translation="MDNTLKPRLIQFPAFGNPAEGYLSVSQFSTGMPFEVKRTFWTYH
                     TPEAVTRGRHAHFETHMVLVAAAGRIIVDLEENDGTTSQHILETPTTGLYMPPMTWHV
                     MKYSHNAIQIVFASTDYDEADYIREYGRFLTLRKHQS"
     gene            complement(3424..5106)
                     /locus_tag="PLE23_06545"
     CDS             complement(3424..5106)
                     /locus_tag="PLE23_06545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMX82957.1"
                     /translation="MAKKPTTPKQNKATQASSSGLSVNFDKYAPHISIGVMILFLIIM
                     FMIRLNFKEMSFERDEGTYSYMGKLLTEGKKPYSYFYEMKPPALYYMYGLIVAIFGTT
                     VAGLHVGFFVVNFITCLFLFLWVRRMFNPAAGAIAAVAYSIISTAPHASGLTIQSEHL
                     LCLFMVPGLWLLWEAFSQNKTWQWLTSGALLSWALLIKQNTLFFIAFAGLAIILHHFL
                     VKKEKGIASLWRPVLLFGAGVIVPVLLVLGILVANGSFKDFIFFIYEYPKTQYLSSIE
                     FSRGMGYMQNRYEAIKTNYEIIWYLGFAGLVLVWVFSKNMFLKISLTLLLIFSFLSVA
                     PGLRFYGHYWIHFLPAVAVLFATAIYIITEKLKEKGVLKNPQFIAILVLCVVFFINLN
                     TNKSYYFITDPETVVRGVYGTNPFVESRKVADYLNNKMQKNDQLVVFGSEPQIYMYTG
                     KDAITRHFYTGNLVQLHPIVKEWSAEFKQDFMKAQPKYVVWVQHPYSWSLPKGASTEF
                     IDWGWDTIKKGYHPIGYADIISHNQTEYVWDNEALNYRPKGNEYLMVLERNN"
     gene            complement(5128..5805)
                     /locus_tag="PLE23_06550"
     CDS             complement(5128..5805)
                     /locus_tag="PLE23_06550"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMX82958.1"
                     /translation="MRWPISFIVFMWAVFFVTIILNVDLGNFGIYPRTTVGFLGIFTS
                     PLIHANFKHLLSNTVPMIGMLTIMVMFYPRVAMKAFLMIYVLTGLSVWCFGRPVYHIG
                     ASGVVYGLVAFVFWSGVFRRSMKSIVLALIIVILYTPMFAGILPNQEGISWESHLLGG
                     IVGIFTSFYYKKYLEVDEMEKDNHIIDDEDETRQYFLPRDTFDLTMEERKRRQDSGNW
                     YSDSTFL"
     gene            complement(5838..6806)
                     /gene="pfkA"
                     /locus_tag="PLE23_06555"
     CDS             complement(5838..6806)
                     /gene="pfkA"
                     /locus_tag="PLE23_06555"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HMX82959.1"
                     /translation="MKKIAVLTSGGDAPGMNACIRAVVRTGTSSGIEVMGIMRGFQGM
                     IDDEFIVLDSQAVSNIIQTGGTILKSARSEEFRTPQGRKKAFDNLKQHNIDGLVAIGG
                     DGTFRGANAFISEYGFPIVAVPGTIDNDLYGTDFTIGYDTAINTAMSAIDNIKDTANA
                     HNRVFFVEVMGRDSGFIALRSAIATGAEAVIIPETPTDIASLAQQLKDNYDRKKRASI
                     IIVAEGDECGGAFKIAQKVKEHNPEFDTKVTVLGHIQRGGRPTCMDRVLASRLGMEAV
                     RALLNGTSGVMIGQLHKKIAHTPLVKAIKHNVTVNPLLVEMVNILA"
     gene            complement(6823..7776)
                     /locus_tag="PLE23_06560"
     CDS             complement(6823..7776)
                     /locus_tag="PLE23_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82960.1"
                     /translation="MNMTQLTELLRKSLQIAETASREQHLSTIEKDLLLSNIRKMYDL
                     LLEWPSHASVNPTERKNTEPAHNAIPDTTVHTRQESVDYKPAPTIDETVHTNTQEQEP
                     APTLPVQTEQTTAPTPTIETTITKQEETVIAEEVKQNPTPEPSTTLQSSPESAKDVVI
                     ETVMAEEVKEEPASKAKPSFDDLVKKVKEQFDGTEKKPTYVAPEDDDDVPVFFSDLRF
                     DKLFDTEYARELSERLGAAKVDDINKAMGLNEKIFTINELFGGDNASFDQAIKTLQSF
                     ATFDQAKEYIIQNIAPQYDWLSPQKFKKAKEFIKIVKRKFA"
BASE COUNT         2175 a         1828 c         1708 g         2407 t
ORIGIN      
        1 aaaggcggcc gcatccgtga tgattgggtt gaaaaacggc attttcagca ccggataaca
       61 ccagaacaag gccgctagtc cgagaaattc gagttctgcc gacagtttca gccgcctgct
      121 gatcaggtaa taatacagta gccccagcat gagcagcaac agattgaaca gccggaatcc
      181 ggcaacaata ttgggcaccg tgaacggaat atccatggcc ttcatcacct tgttgattac
      241 tacagacgtc aacacacgct gaaaacggta tgagttgata gccttttctt ccagcgatct
      301 gtcaaggtgt gccgtataag aagcatacag gcggccatcc catccggccc cttcgttgac
      361 atcaatgagt tcgccacggt agccatagaa caatagggcc agcgttatga gaatacccga
      421 aattctatat gcattcattt ccgaattttt ttattgcttc acaaacataa gaaacctgct
      481 catctttcat acctgcaaac atcggcaaac tcaaacattc gtgggcaagt ttttctgcaa
      541 taggaaacga gccatttccg agattgagat ttttatatgc tttctgcaaa tgtggcggta
      601 ccggatagtg gatcagcgta ccgattcctt gttcgctgag atattgctgg agcttgtcgc
      661 gatatggtgt cgttatcaca aaaagatggt aagcatgtgt agcatgctca tgcgtgtgtg
      721 gcaacaccag gaaatcacaa gacgacaact gtctaagata ttcagccgca gtcttttgac
      781 ggctctccgt ccactcctgt aatcttctga gcttaacaga caaaaatgct gcctgcagtt
      841 cgtccaggcg tgaattgaat ccttgtactt cgttgtaata tttcttttcg ctgccgtaat
      901 tgcgccaaat ctttgtaagt cgggcaaact cggcagaatt cgtcgttatg gcgcccgcat
      961 caccgatggc accgagattt tttccgggat aaaagctcgt tccattgatg tcgccccagg
     1021 aacctgtaaa cttgcctttg taagcggcac cctgcgactg ggcattgtct tctatgacat
     1081 acagattgtg tttcttcgct atttccatca cgacatccat ttcgcaagcc tgaccgtaga
     1141 gatgtaccgg catgatggct tttgttttgg gtgaaatttt ttcctcgatc ttctgaacgt
     1201 caatgttgta agtacgggca tcaggttcca cgaaaactac ctgtgcacca gtgtaagaaa
     1261 ctgccagcgc ggaagctata taggtgttgg caggaacaat cacttcgtca ccttgaccaa
     1321 tattcagaca cttgagcgct atgtgcaatg cctccagacc atttcctacg cctgtgcagt
     1381 atttggcctg gcagaaatct gcaaatgaag attcaaattc attaacatat tcgcccagaa
     1441 taaaggaatt gctgtcatac acggcttcca ttgctgccat catttcttct tttaggggaa
     1501 tgtgtatcgg gtcaaagctc aaaaaaggta ctttcataca tgttctttac aggtaatatt
     1561 caaatgtttc ataacaaacg cctctgccac cgaagccttc cttctgaaaa atcagcgatt
     1621 cgttcagata gtagccgttg tcttcggtcg aagagccaag gtcaaaatat tttttatgac
     1681 tgaattctgt gcgaatcaga tggtcaagaa taaaatcaag cgcacaatga gtttttcccg
     1741 cttcattgat ggaaatatac tgggcatgtg ccactgtatc ggattcgtac actacgatac
     1801 cgcccagaca tttacagtct ttggatgcgg taaacaggtg gatgttttcg ggaaaagatg
     1861 cgtgcaggcg gagaatttca gccaacgagt gaaccggttt tgtgccatat ttctggtgca
     1921 aattttcttc aagaatattc cagaattctt tccagtgttg gtttttgtgc aattcaagcc
     1981 cctgtttcag cgatttgccg accgcatttt tccttgagcg gttgtaggag aaattctcac
     2041 tcagatccag tgtcgtggaa atgttgcagg ctgttatctt cgcgtgattt ctgaacatga
     2101 gataacgatc ctcatctatc ggctgccttg catagatgta gggaatcttc ttgaaaacga
     2161 ctttggaaaa tccgttttcc cttagaaaat ggttgagcaa atccgaaatc ctgagatgcc
     2221 cggctgttgt catatcagtc tgtgtgataa tgcctccgaa tgtaaggcct tggtgcgtgt
     2281 aaaacacatg ttccctacgg ttagccggaa tgattccagc gagtttgccg tttgtatcaa
     2341 acaccatcag cgaaaaatct tcaaacctgt cggcgtggta atccatgtaa ttgcggttat
     2401 ggagaaaagt accattacgt gaagagcgga caaactcatt ccaggtggcg gcatccattt
     2461 tttcatattt tcgaatggaa aacatgggta tttctaccag tttcctcatg actataagct
     2521 ctgtctgatt ttcaattatt tcgaatccga attttttata aagactaatg gcgcgttggt
     2581 tgccattcca tacacgaaga ccgatagccg gaatttttct ttttatagct tcttcgaaaa
     2641 tgaagtcaag tattttactg ccggccccgt tgccccagaa ttgtttttcg ccaatgtagc
     2701 cccaatattc tgcgctctcg gcactaatat ttttcaggcc acaggcacca gccggtattc
     2761 cgtcgatgca gataccccat atcagataat cgttcttgaa cggtaggctg tcgaaccatt
     2821 tctgttggct ttcaggcgta atatcaggag taagtgtcaa ttgtttgatt tccggatcgc
     2881 tgagccaggt gctgcttctg tgaaggaagc tttcatcata cggaacaaac tcagtattcg
     2941 gattcattgg tttatgattg atgttttcgc agagttaaga acctcccata ttcacgtata
     3001 tagtccgcct catcataatc agttgaggca aagactatct gtatggcgtt gtgcgaatac
     3061 ttcatcacat gccaggtcat aggtggcatg tagagcccgg tagtgggcgt ctccaatatg
     3121 tgctgcgatg tggtaccgtc attttcctcc agatctacta tgatacggcc tgcagcagct
     3181 accagtacca tgtgtgtttc aaaatgtgca tgtctgccac gggtcactgc ctcgggtgtg
     3241 tggtaggtcc agaatgtcct tttgacttcg aagggcatcc cggtcgagaa ctgactgaca
     3301 gaaaggtacc cttcagcagg attgccaaaa gctggaaact gtatcaaccg tggtttaaga
     3361 gtattatcca tgccgctatt ctacgatttg cagatatatt aagtttcgca aagctgaata
     3421 acatcagttg ttgcgctcca gcaccatcag atattcattg cctttgggcc tgtaattgag
     3481 cgcttcgtta tcccaaacgt actctgtctg attgtggcta atgatatccg catacccgat
     3541 tgggtggtat cctttcttta tggtatccca gccccagtct atgaactctg tactggcgcc
     3601 tttgggcaat gaccacgaat acgggtgttg tacccacact acatatttag gctgagcctt
     3661 catgaaatcc tgcttaaact ctgccgacca ttccttcaca atcgggtgta gctgcaccag
     3721 attgcctgta taaaaatgtc tggtaatggc atccttgccg gtgtacatat atatctgagg
     3781 ctccgagcca aatactacaa gctggtcgtt tttctgcatc ttattgttga gatagtcagc
     3841 cacttttctt gattcaacaa acggattggt accatacact ccccttacaa cagtctccgg
     3901 atcggtaatg aaatagtagg atttattggt attcaaattg ataaaaaaca caacgcacaa
     3961 tacgagtatc gctatgaact gtgggttttt caaaacaccc ttctctttta gtttttcggt
     4021 gatgatataa attgcggtag cgaataaaac cgccacagcc ggcagaaaat gtatccagta
     4081 atgaccgtaa aatcgcagac caggcgcaac agacaggaat gaaaatataa gcagcagcgt
     4141 taatgaaatt ttcaaaaaca tatttttact gaatacccaa accaatacca gtccggcaaa
     4201 gcctagatac cagatgattt cgtaattggt ctttattgcc tcatacctgt tctgcatata
     4261 tcccatacca cgcgaaaatt cgatactgga cagatattgg gttttggggt attcataaat
     4321 aaagaaaatg aaatccttaa acgacccatt ggcaacgaga atgccgagta ccaacaatac
     4381 tggtacaatg actccggctc caaacaacaa aacaggtctc caaagagatg ctataccttt
     4441 ttcttttttt acgaggaaat gatgtaaaat aattgccagg cctgcaaaag ctatgaagaa
     4501 caaggtgttt tgcttgatga gcaatgccca actgagcaag gcacccgaag taagccactg
     4561 ccatgtttta ttctgcgaaa atgcctccca taacagccaa agtcccggta ccataaataa
     4621 acacaacaga tgctcagact gtatggtgag tccgctggca tgtggcgctg tggaaatgat
     4681 gctgtaagct actgcagcga tagcacctgc tgccggattg aacattctgc gcacccataa
     4741 gaacaaaaac agacaagtaa taaagttgac gacaaaaaat ccaacatgca aacctgctac
     4801 tgttgtacca aaaatggcaa caataagtcc gtacatataa tacagggcag gtggtttcat
     4861 ttcgtagaaa taggaatacg gcttcttccc ttcagtgagc agcttgccca tgtaggagta
     4921 ggtaccttca tcgcgttcga aagacatttc cttgaaattt aacctgatca tgaacataat
     4981 aatcaggaac aaaatcatca caccaatgga tatgtgcgga gcgtatttgt caaaattgac
     5041 tgatagaccg gatgatgatg cttgggtagc tttgttttgt ttgggagtag tcggtttttt
     5101 tgccataatg tgttaaagcg aatttttcta taaaaaagtt gagtctgaat accagttgcc
     5161 actatcctgt cttcgtttgc gttcttccat tgtaaggtca aatgtgtcgc gtggtagaaa
     5221 atactgtctg gtctcgtctt catcgtctat gatgtggtta tctttttcca tttcatccac
     5281 ttcaaggtat tttttataat aaaatgatgt aaaaatgcct actataccac ccaacaggtg
     5341 actttcccat gatatgccct cctgattggg taaaataccg gcaaacatcg gtgtatataa
     5401 aatgactatt atcaatgcca gaactattga cttcatactc cgtctgaaca caccactcca
     5461 aaatacgaaa gccacaagcc catataccac tccgcttgcc cctatatgat acaccggacg
     5521 tccgaagcac cataccgata aacctgtgag tacatatatc atcagaaatg ccttcatggc
     5581 gactctcgga tagaacatta ccatgatggt aagcatacct atcatcggta ctgtattgga
     5641 aagcaaatgt ttgaagttgg catgtatcaa tggtgaagta aaaatgccta aaaagcctac
     5701 cgtagttctt ggatagatgc caaaattacc cagatcgaca ttgagaatga tggtaacgaa
     5761 aaatacagcc cacataaata cgatgaagct tatcggccat ctcattcttt ttttaaatga
     5821 ttgtagtgcc tgtcccatta tgcaagaata tttaccattt ctactaaaag agggttaact
     5881 gtcacattgt gtttgatggc cttgacaagt ggagtgtgag ctattttttt gtgtaactgc
     5941 cctatcatga caccagacgt gccattcagc agcgcccgta ctgcctccat accaagccgg
     6001 cttgccagca ctctatccat acatgttggc cgtccacccc tttgtatatg cccgagtacc
     6061 gtaaccttag tatcgaactc cggattgtgt tctttgacct tctgggctat cttaaatgct
     6121 ccgccgcatt catctccttc tgccacgatg atgatacttg ctcttttctt ccggtcgtag
     6181 ttatctttta actgctgtgc gaggctggcg atgtcggtag gtgtctccgg tatgataacg
     6241 gcttcggcac cggtggctat tgccgacctg agggctatga acccactgtc gcggcccata
     6301 acttccacaa aaaacaccct gttgtgagca ttggcagtat ctttgatgtt atcgattgct
     6361 gacatagctg tgttgatagc agtatcataa ccaatcgtaa aatctgtgcc ataaaggtcg
     6421 ttatcaatag tacccggtac agctactatc gggaagccat attcgctgat gaacgcattt
     6481 gctcctcgaa aagtaccgtc gccacctatg gcaaccaaac catcaatgtt gtgctgcttt
     6541 aggttatcaa atgctttttt tcggccttgt ggtgtcctga actcctcact tctggcagat
     6601 ttcaggattg tgcctcctgt ctgtataata ttactgaccg cctggctgtc aagtactata
     6661 aattcatcat ctatcatacc ctgaaaacca cgcatgatgc ccatcacttc aatacctgaa
     6721 ctggtgcctg tgcgtaccac agccctaatg caggcattca ttcccggagc atcacctcct
     6781 gatgtcaata ctgcgatttt tttcatagcc ggacagcctt ctttaggcaa attttctctt
     6841 tactatttta ataaattcct tagctttttt gaacttctgc ggactcagcc aatcatattg
     6901 gggtgcgatg ttctgaatga tgtactcctt tgcctggtca aatgttgcaa aagactgtag
     6961 tgttttgatg gcttgatcga atgaagcatt atctccgccg aacaattcat tgatggtaaa
     7021 tatcttttca ttgagcccca ttgccttatt gatatcatca actttggcag ctccgagccg
     7081 ctcagaaagc tcgcgtgcat actcggtatc gaagagtttg tcaaacctaa ggtcggaaaa
     7141 gaaaacgggt acatcgtcat catcctctgg agccacataa gttggttttt tctcagtgcc
     7201 gtcaaattgt tccttaacct ttttgaccaa atcatcgaag cttggtttgg cttttgaagc
     7261 gggttcttct ttcacttctt cagccattac cgtctctatt actacatctt ttgctgattc
     7321 aggcgatgat tgtagcgtgg ttgatggttc aggtgtagga ttttgcttta cttcttctgc
     7381 tattacggtt tcttcctgtt tcgtaattgt agtttcgatg gttggtgtag gtgccgttgt
     7441 ttgttctgtc tgcacaggta gtgttggggc tggctcttgc tcctgagtat tagtatgaac
     7501 agtttcatca atcgttggag ctggtttata gtcaaccgat tcttgtctag tatgaacggt
     7561 agtatcaggt atagcattgt gtgcaggctc cgtgtttttc ctctctgtag gatttaccga
     7621 tgcgtgtgaa ggccactcga gcagtaggtc atacattttc cggatattgg acaggagcag
     7681 atctttttca atggttgata aatgttgttc gcgtgaggcg gtttctgcta tctgcagtga
     7741 ttttctgagc aattcagtca gttgggtcat attcataaga tcgggtgttt ttcaggcatg
     7801 aatgaaataa ggcttaaaat tgacaaaaaa tgacggtgtt tctcagaata taattgaaaa
     7861 acttactatt tctgtcaaaa ataataacgt aggaataagc ccgtttcgtc caatgatacc
     7921 caaaattatt ccaaaaccta tgtggaataa tcgcaccggc atatccggtt aataaaaaag
     7981 gtataagcaa atgatataaa caacccgaca gaaaatatcc atttcagcgt caaactcacc
     8041 tcttttccat cactgcttca ccaacttttc tacctgtacg actttcgcat ctttgttttg
     8101 gatcgacaac agatacat
//
LOCUS       DAOQQY010000108         8074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3507_length_8074_cov_6.420626, whole genome shotgun sequence.
ACCESSION   DAOQQY010000108 DAOQQY010000000
VERSION     DAOQQY010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3507_length_8074_cov_6.420626"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="PLE23_06565"
     CDS             complement(<1..1014)
                     /locus_tag="PLE23_06565"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HMX82961.1"
                     /translation="MSIKKRSWAEPFKIKMVELLKMTTPAQRRKAIREAGYNTFLLKS
                     EDVYIDLLTDSGTSAMSDRQWAGLMMGDEAYAGSKNFYTLEKAVQKYYGYKYLIPTHQ
                     GRGAENLLSQSLIKKGDIVPGNMYFTTTRLHQELAGGTFVDVIIDEAHDSQSLHPFKG
                     NIDINKLDAVVKKHGAVKIPYICVAVTVNMAGGQPVSMENLKLLREYTSKHGIKIMLD
                     MTRIAENAFFIQQREAGYSHKSVAEIVREICDLTDGATFSGKKDALVNIGGFLAVNDF
                     ETFEEASNLVVVYEGLHTYGGLAGRDMEAMAIGIAESVHDEHMKARIGQVQYLGEKLQ
                     EAGI"
     gene            1163..1648
                     /locus_tag="PLE23_06570"
     CDS             1163..1648
                     /locus_tag="PLE23_06570"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMX82962.1"
                     /translation="MADPQKTIKTPAYSETIKTEVVCPNDTNPMEILQGGRLVQWMDI
                     AAAVCAQTHADGICVTASIDNVRFYNSAKIGDILTIKARVTRAFSTSMEILVDASVRN
                     PFNHTPKSLAKAYFTFVAIDEHAKKRNIEPLVIQTKEDEKHSRMAAVRKERQLSQQDD
                     E"
     gene            complement(1662..3338)
                     /locus_tag="PLE23_06575"
     CDS             complement(1662..3338)
                     /locus_tag="PLE23_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMX82963.1"
                     /translation="MNFYFKSIIVLITSLFLLEQVQAQCLSGDCKNGKGIYLFPSGAK
                     YIGFFKNGECHGIGVCYFTDGSKYQGNWKNRFPEGHGIKTYSDGTVRNGYWKKGRPVD
                     ENGNLVEEIYIQKESKNDGTDIQAGCLTGNCISGDGVMTYVDGSKYEGQFSKGKITGF
                     GTWYYPNGEKYVGNFSNGLPTGKGTLYKQNGTQLSGSWRDGEYIGEIKEPAAKTGCIS
                     GNCQNGRGVFLDEDTGNAYEGQFVNKLFNGTGIMKYANGDIYNGQWKDGKYYGNGKLT
                     RKDGTSVEGNWIDGEFNPLIVAYGDQGSEPGANAPKLNPGMFHSSRVWAVVVGISQYS
                     HMPVLRYSDDDAYKMYAFLKSPDGGSIPDERIKLLIDEDATKANIRKSLKEMLENAGP
                     DDVVMFYFAGHGLKGSFLPIDFDGYNFKLTHEEFNEIMKNSRARLNLCVIDACHSGGL
                     AADNSISPELMMGAYYDRLTGDGQHTAILLSSKSEETSLESKGLRQGVFSHFLLRGLK
                     GEADLNKDSVVTIKELFEYVSLNVKEYTEDKQTPEIKGEFDAQMPVVFKK"
     gene            3788..4258
                     /locus_tag="PLE23_06580"
     CDS             3788..4258
                     /locus_tag="PLE23_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4920 domain-containing protein"
                     /protein_id="HMX82964.1"
                     /translation="MKLLLVTIMVVSICTGVFAQQKKAKMQYFGEKIKAKNAISYDVM
                     LDKLATTEPLKAKVRGKVQSVCQEKGCWMTIVSDDKSKPVMFVKFKDYAFFVPKNIQG
                     KEAVVEGEAYYEETSVKELKHYAEDEGKSKAEIDAITEPKRELKLMASGVIIIE"
     gene            4272..6455
                     /locus_tag="PLE23_06585"
     CDS             4272..6455
                     /locus_tag="PLE23_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMX82965.1"
                     /translation="MSVVCLRTVFSVFLAILAMGSFAQQAELDSLKNSLKLRQPDTSL
                     INTLNELGFLSLGDDPIQAGKYLTEALDKSVKAKFPKGEANAYVSLAIIASIHSDYKK
                     AHDYYKRAIAIRSRLGDRKGVANIYNNIGNLHDEQGDFFNAIENFKQALEIYQSLGDK
                     TKMGRAQYNISTVYWRMGNYSKAIENVYAYLAIVDKEGDQEGIATANNLLGNLQVDIL
                     KPEKAMSYYQTALKIWIDTKNKEEEANILNNIGSLKDDLGETAMKNGKKSEALQLFNE
                     SIQYLENSLKIRQTRNDEAGISEIYNNLGVVKKNLGTLYLKSNDKILAKKYLNEALQS
                     FKQSLTIREKESDKNGIIEVYNGLGDVYRRLKDYKTAFYYTEAYKKLAEEIGSTKFQQ
                     SAFKDLAQLNASIGNYKSAYENRLNYDSLKDVRMDESNLLKYQQDEFNFKEGKRQREI
                     DQKQSQITLQQEKLKTERTRRNSLLGGAILLGLLTLLLYNRYRIKNKSNKELTEKNII
                     IEQERMRSDQLLMNILPASTALELKTTGAAKAKQYDSVSVLFTDFKNFTQIAEKLNAE
                     ELVAELDHCFKGFDAITTKYGIEKIKTIGDAYMCAGGIPDVNQTHPMDTVMAALEMVK
                     FLHDYQLPKIKQGQPYFEVRIGIHTGPVVAGVVGTRKFAYDIWGDTVNLAARMESSGE
                     PGKVNISESTFELVKDHFKCIHRGKIDAKNKGQIDMYFVEDATQV"
     gene            6493..6858
                     /locus_tag="PLE23_06590"
     CDS             6493..6858
                     /locus_tag="PLE23_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82966.1"
                     /translation="MKKYLLALVCSTILILLTHCSNKPHLNYDQIGKKLCGCSEELSQ
                     KNTKLTQLIEKKQNDKAMLLMDTLETMENKLKACIIEENKLDNFMNDSKSEKALPRVL
                     NKLCPDRSGKIIELVKEIK"
     gene            complement(6869..7429)
                     /locus_tag="PLE23_06595"
     CDS             complement(6869..7429)
                     /locus_tag="PLE23_06595"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMX82967.1"
                     /translation="MEQINYLKNTLMHFRTTGTLVRSSGFLCKTMLSNIDFNNAQNIV
                     ELGPGDGVITRHLLNSMKQDAHLYSFEINEYFLKYLEENFKDPRLSVLPESAEHLIPE
                     LASRGVREVDYVVSAVPFMILPESLTESIVGACHQALKPGGKFIQFHYSLNARKYYQK
                     IFKNIQIRFVPFNMPPAFVMVCEKLG"
     gene            complement(7496..>8074)
                     /locus_tag="PLE23_06600"
     CDS             complement(7496..>8074)
                     /locus_tag="PLE23_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82968.1"
                     /translation="YISLRQAENIRDYLQKNDVNEKQILVRGAGRNFPIIKDDIPANA
                     SVLKQNLSNRVDLAFSQQDDAGIQFEYQELKVMKSLADERYNIYRLSNIGLMYRVQVA
                     ATTQANHSQILDAYPDFIIEKRPKSDLLFYLIGNKDSYSEISLLRTKINNSGVKDAFI
                     VAYVDGWPITRNQAIELKRMYPDLENFIKEGK"
BASE COUNT         2545 a         1713 c         1549 g         2267 t
ORIGIN      
        1 gataccggct tcttgcagtt tttcaccaag atactgcacc tgaccaatac gcgccttcat
       61 gtgttcgtca tgaactgatt cggcaatacc gatggccatg gcttccatat ctctgcctgc
      121 aagccctccg taggtatgca agccttcata aacaacaacc agattactgg cttcttcgaa
      181 tgtctcaaaa tcattcacag caaggaaccc accaatgttt acaagagcat ctttcttgcc
      241 actgaatgtt gccccatctg tcagatcgca gatctcgcgt actatctccg ctacactctt
      301 gtgcgaataa ccggcttcac gctgctgaat gaagaaagca ttctcggcaa tccgtgtcat
      361 atcgagcatt atttttatac catgtttgct ggtgtattca cgaagcagtt tgaggttttc
      421 cattgatacc ggctggccac ctgccatatt aacagtaaca gctacgcaga tataaggtat
      481 tttgacagca ccgtgctttt tgacaactgc atcgagctta ttgatatcga tgttaccctt
      541 gaatgggtga aggctttgcg agtcatgtgc ttcatcaatg atgacatcta cgaatgtacc
      601 tcctgcgagt tcttggtgaa gtcgggtcgt tgtaaaatac atattgcccg gcacgatatc
      661 acctttcttg atcagggact gtgacagaag attttctgct cctctacctt ggtgtgtagg
      721 tatgaggtat ttgtatccgt agtatttttg aacagctttt tcgagcgtat agaaattttt
      781 actgccggca taagcttcat cgcccatcat gagccctgcc cattggcggt cactcatggc
      841 agaagtaccg ctgtctgtca gcagatcgat gtacacgtcc tctgatttca ataaaaatgt
      901 attgtagcct gcttccctga tggctttacg gcgttgtgca ggagtggtca ttttgagcaa
      961 ttcaaccatt tttatcttaa acggctctgc ccatgaacgt tttttgatac tcattgtctt
     1021 tttgtctgca aaattacgct ctgtggtagc cggcatggta tgaccaaaat cataggtggc
     1081 aatgattata ttacgtttct tgcagctgat tttacgggta atgcccgtgt attgaaaatt
     1141 taattatatt caacaaaaaa gtatggcaga tcctcaaaaa acaatcaaga ctccggctta
     1201 ttcggagaca ataaagactg aagtagtatg cccgaatgat accaacccaa tggaaatatt
     1261 acagggcgga aggcttgtgc aatggatgga tattgcagca gccgtatgtg cacagactca
     1321 tgccgacggt atctgtgtga cagcctccat agacaatgta cgtttttata attcagctaa
     1381 aataggtgat attcttacta taaaagccag agtgacacgt gcattttcta cctctatgga
     1441 aatattggtc gatgccagtg ttcggaatcc tttcaaccat acaccgaagt cacttgccaa
     1501 agcatatttt accttcgtag ccatcgacga gcatgcaaaa aagcgaaaca tcgaaccatt
     1561 agtgatacaa accaaggagg atgaaaaaca ttcccggatg gctgctgtaa ggaaagaacg
     1621 ccaactctca cagcaagatg atgagtaaaa acaagtgaaa atcacttttt aaacactaca
     1681 ggcatctgtg catcaaattc tccttttatt tcaggtgtct gtttgtcttc tgtatattcc
     1741 tttacattga gtgatacata ttcaaaaagt tctttgatgg ttactacact atctttgtta
     1801 aggtctgctt caccttttaa cccacgcagt aaaaaatgag aaaacacacc ttgtcgaagg
     1861 ccttttgatt cgagcgaggt ttcctcagat tttgatgaaa gcaagatggc tgtatgttgt
     1921 ccgtcgccgg tgagtctgtc ataataggca cccatcatca gttcgggcga tatgctgttg
     1981 tcggcagcaa gacctccgct atggcaggca tctatgacac aaagattgag cctggcacga
     2041 gaatttttca tgatttcatt aaattcttcg tgggtgagtt taaaattata cccatcgaaa
     2101 tcaataggta gaaaagagcc tttgaggccg tggccggcaa aatagaacat aacaacatca
     2161 tcggggccgg cattttcgag catttccttc aacgatttgc gaatgttggc cttggttgca
     2221 tcttcatcaa taaggagttt gatcctttcg tcagggatgc tgccaccatc cgggctcttt
     2281 aggaaagcat acattttgta ggcatcatcg tctgaatacc tcaacaccgg catgtggctg
     2341 tattgcgaga tgccgactac tactgcccac actcttgaac tgtgaaacat tccgggattg
     2401 agttttgggg cattagcacc tggttcagaa ccttgatcgc cataagctac tatcagagga
     2461 ttaaattcgc cgtcaatcca attgccttct actgaagttc catccttgcg tgtcagcttg
     2521 ccattaccat agtatttacc atccttccac tgtccgttat agatgtcgcc attggcatat
     2581 ttcattattc cggtgccatt gaagagctta tttacgaatt gtccttcgta agcatttccg
     2641 gtatcttcat ccaaaaacac accacgcccg ttttggcaat tgccactgat gcagcctgtt
     2701 ttagcggcag gttcttttat ttcgcctata tattcgccgt cacgccaact accgctgagt
     2761 tgagtaccat tttgcttata gagtgttcct ttgcctgtag gcaatccatt gctgaaattg
     2821 ccgacatatt tttcaccatt aggataatac caggttccaa agccggtgat tttcccctta
     2881 ctaaactgcc cctcgtactt actgccgtca acataggtca taacaccatc tccgcttatg
     2941 caattgcctg taaggcaccc tgcctgaatg tcggttccgt cgtttttgct ttccttctgt
     3001 atatatattt cttctacaag gttaccattt tcatcaaccg gtcgtccttt tttccagtaa
     3061 ccgtttctga ccgtaccgtc agaataagtc tttataccat ggccctccgg gaacctgttc
     3121 ttccagttgc cctgatattt actcccatcc gtaaaataac aaacaccaat accatgacat
     3181 tcaccatttt tgaaaaagcc gatatactta gcaccactcg gaaacaagta aatgcccttg
     3241 ccgttcttac agtcgccaga cagacattgg gcctgcacct gctctaataa aaacaaggaa
     3301 gtaataagta caatgattga tttgaaataa aaattcatgc ccggcagatt catttttggc
     3361 tttgtggatt atttacacac aacaaaataa gtaataaaaa tacatttttc aaaaaattat
     3421 aattccagcc atgcctggtt tattaaaaat ttaacgattt atcaaggtaa aaattttaaa
     3481 cccagctgtc tcaataggaa ttaaacgata atgattcgtg taaataattc gtgcttaagt
     3541 aataaaatca aagtgaacca tggtttttga gtttttggaa tggatttgaa gtaatattta
     3601 ttgaagcaaa gtgtctgatg cgtttttggg cttagaagta accaaggcga tatcaaactt
     3661 tcaatagccc aaaagaaaaa gaaataataa aatatgtgaa ttattgtctt aaaaccttct
     3721 ttccatttat aaaaatgaca gctaaaagct taactttgta atgaactaaa ctattactaa
     3781 catgaaaatg aaactattac tggtcaccat aatggtggtg tccatctgta ccggagtatt
     3841 tgctcaacaa aagaaagcta aaatgcaata tttcggtgaa aaaatcaagg caaagaatgc
     3901 catcagttac gatgtaatgc tcgacaaact ggcaacaaca gagccgttaa aggccaaagt
     3961 tcgcggcaag gttcagtccg tgtgtcagga aaaagggtgc tggatgacca tcgtatcgga
     4021 tgacaagtca aaacctgtaa tgtttgtaaa atttaaagat tatgctttct ttgtaccgaa
     4081 aaacatccaa ggcaaggagg ccgttgtgga aggtgaagca tattatgaag aaacctctgt
     4141 caaagaactt aaacattacg ctgaagatga aggcaaatca aaagctgaaa tagatgccat
     4201 cacagaacca aaacgcgaac tcaagttaat ggcatcaggt gttattatta ttgaataaaa
     4261 aattattgta attgtcggtt gtctgtttac gtactgtttt ttctgtcttt ctggccatcc
     4321 ttgctatggg cagtttcgct cagcaggctg aattggatag tctgaaaaac agcctgaaac
     4381 tcagacaacc tgacacttcg cttatcaata cgcttaacga actcggcttt ctgagtttgg
     4441 gtgatgatcc tatacaagcc ggcaaatatc ttactgaagc actcgacaaa tctgtgaaag
     4501 caaaatttcc aaaaggtgaa gccaatgctt acgtttcact tgccattata gcatctatcc
     4561 attctgatta taaaaaagca cacgattatt ataaaagagc cattgccatt cgcagccgat
     4621 taggtgaccg aaagggcgta gcaaatattt acaacaacat tggtaatctt catgatgaac
     4681 agggcgattt ctttaatgca attgaaaatt ttaaacaggc cttagaaata taccaatcgt
     4741 tgggcgacaa gaccaaaatg ggccgcgcac agtacaacat aagtacagtc tattggcgaa
     4801 tgggcaatta ttcgaaagct atagaaaacg tatatgctta cctggcaatc gtagataagg
     4861 aaggtgacca ggaaggaata gccactgcaa acaacctgct cggcaacctg caagttgaca
     4921 ttcttaagcc tgagaaagct atgagttatt atcagaccgc actaaaaata tggattgata
     4981 ccaaaaataa agaagaagaa gccaatatac tcaacaacat aggtagtctg aaagatgatt
     5041 tgggtgaaac cgcaatgaag aatggaaaaa aatcggaggc cctccaatta ttcaacgaat
     5101 ctatccaata ccttgaaaat tcactcaaaa tccgacagac cagaaatgac gaagcaggca
     5161 tcagtgaaat atacaataat ctgggtgttg ttaaaaaaaa tctgggtaca ttgtacttaa
     5221 aatccaacga caaaatcttg gcaaaaaaat atctgaatga agccttgcaa tcattcaagc
     5281 aatcattgac tattcgggaa aaagaatcag ataaaaacgg catcattgaa gtatataacg
     5341 gcctgggcga cgtgtaccgc agactcaagg actacaagac ggcattttat tataccgagg
     5401 cctacaaaaa actcgcagaa gaaattggca gcacaaaatt tcagcaatcg gcattcaagg
     5461 atctggcaca attaaatgcc agtatcggca attataaaag tgcttatgaa aacagactaa
     5521 attatgactc gctcaaggat gttcggatgg acgaatcgaa tctactgaaa taccagcaag
     5581 acgagttcaa cttcaaggaa ggaaaaaggc agcgtgaaat agaccagaaa caaagccaga
     5641 taacattaca acaagaaaaa ttaaaaaccg aacgaaccag acgaaattca cttttgggtg
     5701 gagcaatatt gcttggacta cttacacttt tgctgtacaa ccggtaccgc ataaagaaca
     5761 aatcaaacaa agaactgact gaaaaaaaca tcatcatcga acaagaaaga atgcgttcgg
     5821 accaattgtt gatgaatata ttaccggcca gcactgcgct cgaactcaaa actaccggag
     5881 cagccaaagc aaaacagtat gattctgtat cagtactctt taccgatttt aaaaacttta
     5941 cacaaatagc cgaaaaacta aatgctgaag aactcgtggc tgagctagat cattgcttca
     6001 aaggttttga tgcaattact actaaatacg gtatcgaaaa aatcaagact atcggtgatg
     6061 cttacatgtg tgccggcggc ataccggatg tcaaccaaac ccacccaatg gatacagtga
     6121 tggcggcatt ggaaatggta aaatttctcc acgactacca gttacctaaa ataaagcaag
     6181 gccaaccata tttcgaagta cgcataggca tccatacagg gccggtggta gcgggtgtag
     6241 ttggtaccag gaaatttgct tacgatatct ggggcgacac cgtcaatctc gctgcaagaa
     6301 tggaatcaag cggcgaaccc ggaaaagtaa atatctctga gtcaaccttt gagctggtta
     6361 aagaccattt caaatgtatc caccgtggca aaatcgatgc taaaaataaa ggacagatcg
     6421 atatgtattt tgttgaagat gccacacaag tctgaaatga tgtttttaaa aatccttcca
     6481 ttttctataa gaatgaaaaa atatttacta gccctggttt gtagcacaat cctaatactc
     6541 cttacacatt gctccaacaa accccacctt aactacgatc aaataggtaa aaaactttgt
     6601 ggctgctcag aagagctttc gcaaaaaaat acaaaactca ctcaactcat cgagaaaaaa
     6661 cagaatgaca aagccatgtt gttgatggac acactcgaaa caatggaaaa caaactgaag
     6721 gcctgcatca ttgaagaaaa taaattggat aatttcatga atgactcaaa atcagaaaag
     6781 gcactgccgc gggtacttaa taaattgtgc cccgacagaa gtggcaaaat cattgaactc
     6841 gtaaaagaaa taaaataata cttacacttc aaccaagttt ttcgcatacc ataacaaagg
     6901 ccggtggcat attgaacgga acaaaacgta tctggatgtt tttgaatatt ttttgataat
     6961 attttctggc attgagcgag taatggaact gaataaattt accaccgggt ttcaaagcct
     7021 gatggcaagc accaacaata gattctgtaa gactttcggg caatatcatg aaaggtacag
     7081 ccgacaccac ataatcaacc tcacgaacac cacgcgatgc caactcaggt atgagatgtt
     7141 cggccgactc aggcaataca cttagtctgg gatccttaaa gttttcctct agatatttaa
     7201 gaaagtattc attgatttcg aatgaataca aatgtgcatc ctgcttcata ctgttgagca
     7261 gatgtcttgt aataacacca tcaccgggcc ctagttccac aatattttga gcattgttaa
     7321 aatcaatgtt gcttaacatg gtcttgcaca aaaaacccga actcctgaca agtgtgcctg
     7381 tcgtcctgaa gtgcatcagt gtatttttca gataattgat ttgctccatc tgtttcactc
     7441 ttttttaatg ttccgtatca tctctttgca ttctgataca aaaaccaaac cgattttatt
     7501 tgccttcctt tataaaattt tctaaatccg gatacattcg ttttagctca atagcctgat
     7561 tcctggttat cggccaacca tcaacatacg caacaataaa cgcatccttc acgccgctat
     7621 tgttaatttt agtacgaagt aaactaattt ctgaataact atctttgttg cctataagat
     7681 aaaacaacaa atctgacttt ggtctttttt cgataataaa atccggatag gcatcaagta
     7741 tctgcgaatg attagcctgt gtggtggctg ctacctgtac acggtacatc aagccgatgt
     7801 tagacaatcg atatatattg tacctttcgt cagccaggct cttcataact tttaactcct
     7861 gatactcaaa ttgtatgccg gcatcatcct gttggctgaa agcaagatcg actctgttgg
     7921 acaaattctg cttcaacacg ctggcatttg caggaatatc atcttttatt attggaaaat
     7981 tacgtcctgc accacgtacc aaaatctgct tttcatttac gtcattcttt tggagataat
     8041 cgcggatatt ttctgcttgc cgaagggaga tata
//
LOCUS       DAOQQY010000109         8039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3524_length_8039_cov_6.826653, whole genome shotgun sequence.
ACCESSION   DAOQQY010000109 DAOQQY010000000
VERSION     DAOQQY010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3524_length_8039_cov_6.826653"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="PLE23_06605"
     CDS             complement(<1..1058)
                     /locus_tag="PLE23_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82969.1"
                     /translation="MSHTFRLRFFILLILGLLQTLNIDAQETNANQGSNSGLKADKLK
                     ISGYAQIQYRYIQGNEPDSRFMIRRGRIKFTYEFNHVTLVVNPDFTERKFSLRDTYIE
                     FREPWLNVFSVSLGVLSLPFGYEVKYSSSNRESPERAFINQTLFPGERDLGLCLNVKA
                     PEKSAFNGLELDVAVLNGSANVTPEFDRNKDFLERLSFNRNFKNNNINLGLGLSNYHG
                     GYTIGKVKDFIFEENMKRFQYSPDTADYYRIAKRNYTGADVQFSVNLGNAGKTTLRAE
                     YCMGGQAGTANNSRSAEEIPAEDIYHRKFTGAYFYFVQDIGRLPLQLVAKYDWFDQNS
                     VIAGKEIGVVGTNTNKGD"
     gene            1257..1952
                     /gene="radC"
                     /locus_tag="PLE23_06610"
     CDS             1257..1952
                     /gene="radC"
                     /locus_tag="PLE23_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HMX82970.1"
                     /translation="MMYAPNLTIKSLAEDDRPREKMMLKGRSALSDSELMAILIATGT
                     KNESALMLAQRLLAASNNRLDELGKLSIQELKKFKGIGEAKAITLLAALELGRRRQSS
                     TREENKPIKNSADAYHIFKQHLTDLKVEEFWALYLNRANKNVATKRISTGGMTGTVVD
                     TRIIFKDALDHGATSMILAHNHPSGQLRPSQSDIDLTKKLVAAGKIMDVHVVDHVIVC
                     NSDYFSFADQGLL"
     gene            2118..3272
                     /locus_tag="PLE23_06615"
     CDS             2118..3272
                     /locus_tag="PLE23_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMX82971.1"
                     /translation="MLIQLSEEHLAVQQAAREFAQKELKPGVIERDSKMIYPKEQVKM
                     MGELGFLGMMVSPKYNGGGMDTLSYVLAMEEISKVDNSCSVIMSVNNSLVCWGLETFG
                     TEDQKMKYLTKLATGEWIGAFCLSEPEAGSDATSQRTTAVDMGDHYLLNGTKNWITNG
                     GTSNVHLVMAQTHPELGPKGINALIVETSWPGVTIGAKEDKLGIRSSDTHTIMYTDVK
                     VPKENRIGDDGFGFTFAMKTLAGGRIGIASQALGIAGGAYELAVAYSKERAAFGKPIS
                     QHQAIAFKLADMATKVEAAKLLVYKSALMKDQHLDYGMASSMAKVFASEAAMSISVEA
                     VQVHGGYGYVKEYHVERLMRDAKITQIYEGTSEVQRIVISREVLKGKLYS"
     gene            3300..4424
                     /locus_tag="PLE23_06620"
     CDS             3300..4424
                     /locus_tag="PLE23_06620"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMX82972.1"
                     /translation="MFRSFRLFKLAGIPVYIHWTFFLLIAFVIFQSISASESMIQLGF
                     ELLVLLSVFACVTMHEYGHALTAKRYGVSTQDIILSPIGGVARLNHLPEKPKHELFVA
                     IAGPLVNVAIILLILLTVFVFYPADSFNPGFESISASNFFENYLFVLQYINVMLVLFN
                     LIPAFPMDGGRVLRSLLAMKLGRLKATRIAAFIGQGIAVIFIVSAIFKYPILGRPVST
                     VEQFTLSIIGLFVFSMARAELNSVTAENKVNTIKASDLMVRQFTHLYKTDSTDFAVSQ
                     HNAGIERDFLVFTPDNQVIGTLNNEIIQFIASRGITGLVASYYTPMPTTLTPDEPLKS
                     IAGKTSNLPTQLFPVLENDKVVGVVYKRAVEQQLKEIFRT"
     gene            complement(4421..4933)
                     /locus_tag="PLE23_06625"
     CDS             complement(4421..4933)
                     /locus_tag="PLE23_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMX82973.1"
                     /translation="MSVFKFAFAAMMLCATLNLNAQEYKFGHLNSGNLLASMPEAKAA
                     DEQLAALQKSLVAKGDTMVAEFRKAYDSYLKESSEGILNPKQSKDREDGLHKMQENLQ
                     TYEQQIQQEILKKRQELLGPMLKKVDEAVDAVGKEGKYHMIFDESGGAILYTLPSQDV
                     TELVKKKLDL"
     gene            complement(4959..5177)
                     /gene="tatA"
                     /locus_tag="PLE23_06630"
     CDS             complement(4959..5177)
                     /gene="tatA"
                     /locus_tag="PLE23_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMX82974.1"
                     /translation="MTTFPLIFNLGSWEVVVILFVILLLFGGKKIPELARGLGKGIRE
                     FNDAKSNIENEIKQGMRESERKKIEEEK"
     gene            complement(5190..6137)
                     /locus_tag="PLE23_06635"
     CDS             complement(5190..6137)
                     /locus_tag="PLE23_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HMX82975.1"
                     /translation="MANRAIPLVDLSQYVNGTEEQKKDFVQQLGKAFTEIGFVGVINH
                     GIPKDQIEKFYAESKSFFALPEEIKRKYEIPELAGQRGYTSFGKEHAKQSQHADLKEF
                     FQIGQEPESDAGGVYPSNVKINEKPEFTESGMQLYRSFEQAGGHLLRAIAEYLNLDKD
                     YFDAYIRNGNSILRSIHYPPITQEPASAIRAEQHEDINLITLLVGASAGGLQVLTMDN
                     EWLAVVPESDEIVVNVGDMLQRHTNNKLKSTTHRVVNPPREEWHNPRLSIPFFLHPVS
                     EMKLNCLDSCISDNNSIKYGPITAGEYLDERLREIGLKK"
     gene            complement(6214..7170)
                     /locus_tag="PLE23_06640"
     CDS             complement(6214..7170)
                     /locus_tag="PLE23_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82976.1"
                     /translation="MSNVKVDVDIRRFEKDLFSIDTNQFDQGLKALSAKYGEFADIYF
                     KNVLGAYDKRIAPEGPEQYIRGFVAAPVVRKLYDTCQIVYRDFGTIEKQFENSFKYVK
                     HYFPKKRLPVITTFISEYSLAVFIYGKGDLGVGLDFFLGSKYPYASINYENPNFSSYL
                     TRTYNKDHLVSKAMTALVGDWVDDAPARNRLIDYMIHNGKKLYILHKILPETPDTVVY
                     EYSKKQLDWMKKNEANMWAFFTSENLLYNEDYQKIRKYIEYSPNAPGMPAEAPGKTAN
                     WIGERIIAAYMKNNPGMTMDGLLRQNDAQKILEMSKYKPRRN"
     gene            7360..>8039
                     /locus_tag="PLE23_06645"
     CDS             7360..>8039
                     /locus_tag="PLE23_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMX82977.1"
                     /translation="MKRYFLFFILLAVILPSSYSQDKFRFGFETSPFISWMKTDNKKT
                     IGTGSRIGFNLAANGEYIFAEHYAVRLGLGLSFGQGGGLKHNEINGKAPLFPNSSLSD
                     PILDSIASGTIVRYRINYLEIPFALKMKTPEYGNIRYFAEVPALSIGFKTGAKADIGS
                     YDDENIKKDMAALSLNWGLGIGAEYSLNSSTSLVGGLYYQAGFTDVTSDDGADKSKAT
                     NQRIVLRVG"
BASE COUNT         2311 a         1832 c         1496 g         2400 t
ORIGIN      
        1 atatctcctt tattggtatt ggttccgact acgccaattt ctttgccggc aataaccgaa
       61 ttttgatcaa accaatcata ttttgctacc agttgcaaag gcaatctgcc aatatcctga
      121 acaaaataaa aataagcacc tgtaaatttt ctatggtata tatcttcagc tggaatttct
      181 tcagcactcc ggctgttgtt tgcagtaccg gcttgtccac ccatacagta ttctgctcta
      241 agggtagtct tgccggcatt ccccagattc actgaaaact gaacatctgc accagtataa
      301 tttcgcttcg caatacggta ataatcagct gtatccggcg aatattggaa tcgcttcata
      361 ttttcctcaa aaataaaatc cttaacctta ccaatcgtat agccaccatg ataattagac
      421 aaaccaagac cgagattaat gttgttgttt ttaaaattcc tgttaaatga aagacgttcc
      481 agaaaatctt tattccggtc aaattccggt gtaacatttg ctgaaccgtt caatacagcc
      541 acatcaagct caagtccatt aaacgctgat ttttcgggag ccttaacatt caggcataat
      601 cccaggtctc gctcgccagg gaataaggtt tggttaatga aagcacgttc gggcgattct
      661 ctgtttgatg aagaatattt cacctcatac ccaaacggca aggaaaggac acccagactg
      721 acagaaaata cattaagcca aggttctctg aattcgatat aggtgtctcg aagtgagaat
      781 tttctctccg taaaatcggg attcacaaca agcgttacat gattgaattc ataggtaaat
      841 ttgattctgc cacgtcttat cataaaccga ctatcgggtt catttccctg tatatatcta
      901 tattgaatct gtgcgtatcc ggatatcttc aatttatcag ccttaagtcc gctgtttgac
      961 ccttggttgg cattcgtttc ctgtgcatca atattgagtg tctgaagtaa acctagtatc
     1021 agtaaaatga aaaatcgcag tctaaatgta tgactcataa tatcttaaat tattatactt
     1081 ataccgaaat catggtttgt accttgcaaa tatcgtttta ttcttaacta tcttaattct
     1141 aaatcagcta atattttaaa ttttagcatg ttacttaatt ttggtaaaaa attttatccg
     1201 gttcttatgg aatttgtatg ctcactgcgt caatgttaca gaaagggaaa caatatatga
     1261 tgtacgcacc aaaccttacc atcaaatcgc ttgcagaaga tgaccggccg cgtgaaaaga
     1321 tgatgttgaa aggcagatcc gcattgtctg actcagaatt aatggctata ttaatcgcta
     1381 caggcaccaa aaatgaaagt gctttaatgc ttgctcaaag attacttgcc gcatccaaca
     1441 accgcctgga tgaactgggt aaactcagta ttcaggaact taagaaattc aagggaatag
     1501 gagaagcaaa agcaataact ctgctggcag cacttgaact aggtagaaga cgacaatctt
     1561 caacaaggga agaaaacaaa ccgatcaaaa actcggctga tgcctatcac atattcaagc
     1621 aacaccttac cgacctgaag gtggaagaat tctgggctct ctacctgaac agagcaaaca
     1681 aaaatgttgc taccaaacgg atttcaacag gaggcatgac aggtacagtc gttgatacta
     1741 gaataatttt caaagatgca ctcgaccacg gtgccacaag tatgatttta gctcacaacc
     1801 acccttcagg ccaactcaga ccaagccagt ctgacattga tctgaccaaa aagctcgttg
     1861 ctgcaggcaa aatcatggat gtgcatgttg ttgatcatgt catcgtatgc aattcggact
     1921 atttcagttt tgcagatcag ggcttgcttt agcattttct actacatgga atgacacatt
     1981 ttggtaatat tcaaacagac ttctgtctta cgattttaag taaaaactta aacaaaactt
     2041 actgtttaaa gaaaaaattt ttaactttgc taacctgaag atcattccaa acgtagaact
     2101 cataaaacaa agaaattatg cttatccaac tatctgagga acacctcgcc gtacaacaag
     2161 ctgcacgaga atttgcacaa aaagaactca aacctggtgt catagagcga gactctaaaa
     2221 tgatttaccc taaagaacag gtgaaaatga tgggcgaact cggtttcctc ggtatgatgg
     2281 tatcacctaa atacaacgga ggtggtatgg atactttgtc ctacgtgctc gcaatggaag
     2341 agatttcaaa agtagataac tcatgttcgg ttatcatgtc cgtcaataac tctttggtct
     2401 gttggggtct ggaaaccttt ggcactgaag accaaaaaat gaaatacctg accaaattag
     2461 ctaccggtga gtggattgga gcattctgtc tttcagaacc ggaagcaggt agtgacgcta
     2521 cttctcaaag gacaacagct gtcgatatgg gcgaccatta ccttctcaac ggtaccaaaa
     2581 actggattac caacgggggc acttccaatg tccacctcgt aatggctcaa acgcatcctg
     2641 aactcggccc caaaggtatc aacgcactca tcgtcgaaac aagctggccg ggtgttacca
     2701 ttggtgctaa agaggataaa ctcggcatcc gctcttcaga tacacatacc atcatgtaca
     2761 ccgacgtcaa agtaccaaaa gaaaacagaa tcggagacga tggtttcggt tttacttttg
     2821 ctatgaaaac cctcgccggt ggtcgtattg gtatcgcttc acaagctctt ggtatagcag
     2881 gcggagctta cgaattggca gttgcatatt ctaaagaaag ggcagcattc ggcaaaccaa
     2941 tcagccagca ccaagctatt gcattcaaac tggccgatat ggctacaaag gtagaagcag
     3001 caaaactact tgtttacaaa tcagctctca tgaaagacca gcacctcgac tatggcatgg
     3061 caagcagtat ggcaaaagta ttcgcctctg aagcagctat gagtatttcg gttgaagctg
     3121 tacaagtaca cggaggctat ggctatgtga aagaatacca cgtagaacga ctcatgcgcg
     3181 atgccaaaat aacgcagata tacgaaggaa cgagcgaagt acaacgcatt gtaatttcac
     3241 gcgaagtact caaagggaaa ctttatagct aatccaattg tttcgtacca aaaaaatata
     3301 tgttcagatc tttcaggctg tttaagcttg ccggcattcc ggtttacatt cactggactt
     3361 tcttcctact gatagccttt gttatttttc aaagtatatc cgcttccgaa tctatgattc
     3421 agcttgggtt tgagctacta gtattgcttt cggtctttgc gtgtgtcacc atgcacgaat
     3481 acggccatgc ccttactgct aagagatacg gcgtgagtac tcaagacatt atcttgtcac
     3541 ccataggcgg tgttgccagg ctcaatcacc ttcccgaaaa acctaaacat gaattattcg
     3601 tagccattgc aggcccgttg gtgaatgtgg caattatact gcttatcttg ctcacagtat
     3661 ttgttttcta tcctgctgac agtttcaatc ctggttttga atccatttca gcgtccaatt
     3721 tttttgaaaa ttacctgttc gtcctgcagt atatcaatgt aatgctcgta ttgttcaacc
     3781 tcataccggc tttcccgatg gacggtggca gggtgcttcg ttcattgctc gccatgaaac
     3841 tcggcagact caaagccacc cggattgctg catttatcgg gcagggtatc gctgttatct
     3901 ttatcgtctc ggccatattc aaatacccaa tacttggcag accggtgagt accgtagaac
     3961 agttcactct ttctattata ggcctttttg tcttttcaat ggcaagagca gagctcaata
     4021 gtgtcactgc cgaaaataag gtcaacacca tcaaagcttc tgatctgatg gtaagacaat
     4081 ttacacattt atacaaaaca gacagtaccg acttcgcagt tagtcagcac aatgctggta
     4141 ttgaaagaga ctttctggta tttacacctg acaatcaagt aatcggtaca ctcaataatg
     4201 aaatcataca gttcattgca agccgtggta tcactggttt ggtagcatct tattatactc
     4261 ctatgcccac aacactcaca cccgacgaac cgctgaagtc aatagccggc aaaacaagta
     4321 atctgccaac acaactgttt cctgtgttag agaatgataa ggtagtaggt gtagtgtata
     4381 aaagagctgt tgaacaacag ctaaaagaaa tattccgaac ctaaagatcc agcttctttt
     4441 tcacaagttc tgtaacatcc tgtgatggta gagtgtataa tatcgcccca cccgattcat
     4501 caaaaatcat gtgatatttg ccttccttgc caaccgcatc tactgcttca tccacttttt
     4561 tgagcatcgg gcccaataat tcctggcgct tcttaagtat ttcttgctgt atctgttgtt
     4621 cgtaagtctg aaggttttcc tgcattttgt gcaagccatc ttcacgatct tttgattgct
     4681 tcggatttaa gatgccttcg ctgctttcct tcaggtaact gtcgtaagcc ttccgaaatt
     4741 cggctaccat agtgtctccc ttggctacca gacttttttg taatgcagcc aactgttcgt
     4801 cagcagcctt tgcttcgggc attgaagcaa gcagattgcc ggagttcaag tgtccgaact
     4861 tatattcctg tgcattcaag ttcaatgttg cacacaacat cattgccgca aaggcaaatt
     4921 taaatacgct catctttgta gttaaaaaag tgttcttgtt atttctcctc ttctattttc
     4981 ttgcgctcgc tctcacgcat accttgtttt atctcattct cgatgttact cttggcatca
     5041 ttaaactccc gaataccttt tccgagccct cgtgccagct caggtatttt tttgccgccg
     5101 aacaaaagaa ggattacaaa aagtattact actacttccc atgatccgag attaaatatt
     5161 aatggaaatg ttgtcatgtt attgaatttt tattttttta aacctatctc ccgaagtctt
     5221 tcatcaagat attctccggc agttattggt ccgtatttta tagagttatt atctgaaata
     5281 caactatcaa gacaattaag tttcatctca ctcaccggat gtagaaaaaa cggtatggac
     5341 aaacgtggat tgtgccattc ttctcttggt gggtttacca ctcggtgtgt tgtggactta
     5401 agcttattat tggtatgccg ttgaagcata tcacctacat tcactactat ttcatcactt
     5461 tcaggcacta ctgccagcca ttcgttgtcc attgtcaaca cctgtaggcc tcctgccgat
     5521 gctccaacga gcaatgttat aagattaatg tcttcatgct gctcggcgcg gatggcggat
     5581 gccggctcct gcgtgatagg tggataatgt attgacctca gtattgaatt accatttctt
     5641 atgtaagcgt caaaataatc cttatctaga ttcagatatt ctgctatcgc tcttagcaag
     5701 tgcccacctg cctgctcaaa tgaccggtat aattgcatac cagattctgt aaactcaggc
     5761 ttttcattaa ttttcacatt tgatggatat acaccacctg catcactttc aggttcttga
     5821 cctatctgaa aaaattcctt caaatcagca tgctgtgact gcttggcatg ctccttaccg
     5881 aatgaagtat aacctcgttg cccggcaagt tcaggtattt catacttcct ctttatttcc
     5941 tccggaagtg caaaaaatga cttcgattca gcataaaatt tctcaatctg gtctttagga
     6001 atcccatgat tgatgacgcc tacaaagccg atttcagtaa aggccttgcc caattgctga
     6061 acgaaatcct tcttctgctc ttctgtacca tttacgtact gtgacaaatc aactaaaggt
     6121 attgctctgt ttgccataac taataaatat tgacgtgcaa agttacaata aaatattcat
     6181 ttaaatctta aacgactttt ttaacaaata attctaattt ctccttggtt tatacttcga
     6241 catctcaaga atcttctgtg catcattttg ccttagcaaa ccgtccatcg tcatccccgg
     6301 gttattcttc atataagccg ctattatcct ttcacctatc cagttagctg ttttgccggg
     6361 tgcttcagca ggcattccgg gtgcattcgg gctgtactcg atatattttc ttatcttctg
     6421 ataatcttca ttgtacaaca gattctcact tgtaaagaat gcccacatat tcgcttcatt
     6481 cttcttcatc cagtctagct gcttcttcga atactcatat acaacagtgt ctggtgtttc
     6541 aggtagtatt ttgtgtagta tgtatagctt tttgccattg tgtatcatgt agtctatcag
     6601 tctgttccgc gcaggggcat catccaccca atctcctacc agtgctgtca tagccttcga
     6661 taccaaatgg tctttattat aggtgcgtgt cagatagctc gaaaaatttg ggttttcata
     6721 attgatgctc gcataaggat atttcgagcc aagaaaaaaa tcgagcccaa caccaaggtc
     6781 accctttccg taaataaata cggccagact gtattctgat atgaatgtgg taatcaccgg
     6841 caatcttttt ttcggaaaat aatgttttac atacttgaat gaattttcaa actgcttctc
     6901 tatcgtacca aaatccctgt aaactatctg gcaagtatcg tacaacttcc gcactaccgg
     6961 tgccgctaca aatcctctga tatattgctc tggaccctcg ggcgcaatcc gcttgtcata
     7021 agctcccagc acattcttga aataaatatc tgcaaactcc ccatactttg cactcaaagc
     7081 tttcaatccc tgatcaaatt ggtttgtatc tatactgaaa agatctttct caaatcgccg
     7141 tatatccaca tctactttca cattactcac atccggtact ggttcttttg gctcggaact
     7201 gcacgaactc aagaaaaatc cgaaaatcag aagaatagtt atcgaaaaag cgttattttt
     7261 gtgccccatc tgagtctaaa acttataaat tgtttaatac ttcaactatt tgacaataaa
     7321 ttgaatgcaa aggttgaatt aatcttttaa atttttacta tgaaacgtta ttttctgttt
     7381 ttcattttac tcgctgtcat cctaccatct tcctatagtc aggataagtt ccgttttggt
     7441 ttcgaaacta gcccttttat ctcctggatg aaaaccgata acaaaaaaac catcggcaca
     7501 ggctcgcgca taggttttaa tctggctgcc aacggcgaat atatcttcgc agaacactac
     7561 gccgtcaggc tcggactcgg actgtcattc ggccagggtg gcggactaaa acacaacgaa
     7621 atcaacggca aggctccctt gttccctaat tcaagcctat ccgatcccat cctcgactct
     7681 atagcttcgg gcaccatcgt cagataccga atcaactacc tcgaaatacc cttcgcactc
     7741 aagatgaaaa cacccgaata cggcaacatc agatactttg ccgaagtacc ggcacttagc
     7801 atcggcttca aaaccggcgc caaagcagac attggcagtt atgatgatga aaacatcaag
     7861 aaagatatgg ccgctctcag cctcaattgg ggccttggta tcggtgccga atactccctc
     7921 aatagcagta cttcgctcgt tggcggtctg tattatcagg ctgggtttac agatgtgact
     7981 tcagacgacg gtgccgacaa aagtaaagcc accaatcagc gtattgtact cagggtagg
//
LOCUS       DAOQQY010000110         8005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3552_length_8005_cov_7.190692, whole genome shotgun sequence.
ACCESSION   DAOQQY010000110 DAOQQY010000000
VERSION     DAOQQY010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 8005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8005
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3552_length_8005_cov_7.190692"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="PLE23_06650"
     CDS             complement(<1..1247)
                     /locus_tag="PLE23_06650"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMX82978.1"
                     /translation="MRFIVLVFALVLLSCSTSKKATQAIIPDEIVNLDNLEVVPEDQE
                     EAVAAEDSTIEPLPEYKPSYSREIDLLHTKLEVSFDWAKEQVLGKAALVFKPYGYDIN
                     RFRLDAKNFIINSVKIGNTNLTYNYDDTNLYINLDKTYTPNDEVTIVIDYIARPSEFP
                     AGSSAAITSEKGLFFIDPKNEDPDKPTQIWTQGEAENNSRWFPTIDRPNERCTQELYI
                     TVDKKYKTLSNGLLVSSKVNTDGTRTDYWKMDLPHAPYLFMMAIGEYAVVKDQWKDIP
                     LEYYTEPEYEKDAKLIYNHTPEMLEFFSNTFGLKYPWPKLSQIVVRDYVSGAMENTTA
                     IIYGEFIQRHADELEENPNDYILAHEISHHWFGDYVTCESWANLTLNEGFANYSEYLW
                     FEHKYGKDEAELHRLNELDGYMA"
     gene            complement(1342..2373)
                     /locus_tag="PLE23_06655"
     CDS             complement(1342..2373)
                     /locus_tag="PLE23_06655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMX82979.1"
                     /translation="MPTTQHSLGRFLIIQTASIGDVILATPVAEKLHSFYPHASIDML
                     VKKGNEPLFDRHPFIRKILVWDKRRAKYQNLLKLIKIIRSEQYDCVINIQRFASSGFI
                     TALSGAPLKLGFDKNPLSVLFNVRIKHQIGTGSKHEIERNLQLIESITDRSNKMPRLY
                     PDSSVYASVLTYKQMPYITISPASLWFTKQFPEERWAEFVGQLNSNVVVYFLGAPADT
                     ALCDRIIAAASHQLSVNLAGRLSLLQSAALMKDAIMNYVNDSAPMHLCSAVNAPTTAV
                     YCSTIPEFGFGPLADDSVVVQVESGLYCRPCGLHGYQACPEKHFKCAYDTDISLLINR
                     ISDKFNQTD"
     gene            complement(2384..3202)
                     /locus_tag="PLE23_06660"
     CDS             complement(2384..3202)
                     /locus_tag="PLE23_06660"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HMX82980.1"
                     /translation="MNKLQDIIESAWQDRTLLSQPDTISSIEQVIELLDKGKKRIAEK
                     TDSKGWVVNEWIKKAVLLYFPIRKMETFEAGPMEFHDKIPLKKDYAAQGVRVVPHAIA
                     RYGAHLESGVIMMPSYVNIGAYVGTGTMVDTWATVGSCAQIGRNVHLSGGVGIGGVLE
                     PVQASPVIIEDGCFIGSRCIVVEGVLIEKEAVLGANVVLTASTHVIDVTGSEPVVFKG
                     IVPERSVVIPGSYPKTFPAGTYHVPCALIIGQRKPSTDLKTSLNDVLREYNVAV"
     gene            3395..7405
                     /locus_tag="PLE23_06665"
     CDS             3395..7405
                     /locus_tag="PLE23_06665"
                     /inference="COORDINATES: protein motif:HMM:NF025068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallo-peptidase"
                     /protein_id="HMX82981.1"
                     /translation="MTGIKKTLLYCFIALISITNAEAQWKQIKNSQTITNVGKTVATV
                     EKAAIFSLDKQFLNEISMLKLSGDSKNDGESKIISLPLPDGKYYTVSLHQTALFHPGL
                     LKKYPGIQSFTGYGIGDAFSLVKCSIGEDGFHGMIISPKYGNIFIDPLTDATSSDYIV
                     YNKVDYSIDGKNGFECTLSHANTGRPYSQVDAYHAQKPDCTFRKYRLALACTGEYAAF
                     HGGTIAKVLSAMNTTMTRVSGVYERDLAVSFEIIPDNDKLVFLNGQTDPYSNNNGGAM
                     LYQNQQTVDNLIGEDNYDIGHVFSTGGGGIASLQSVCNSGFKAQGVTGSSAPVGDPFD
                     IDYVCHEMGHQFNAYHTFNNACGGNIAVETAFEPGSGTTIMGYAGVCDPNVQFNSDAY
                     FHSISLSQISTYITKGFGSVCPVKTNLNNTAPTVSAGGDKIIPKSTAFELTATASDAD
                     GDSLTYCWEQMNNELVIHPPLSTYSNGPSFRSFYPTASPTRVFPQITTIINNSTSKWE
                     VLPAVARNLDFTVTVRDNHNGGGCFGTDEMSIKVDGSSGPFLVTKPNTALVWTIGNTE
                     NITWDVAGTDQVPVNCFAVDIYLSVDGGYTYPYLLASNVPNTGNYTVQVPNYPGTKNR
                     IKVKGYGNVFFDISNTNFTISQPNSPSVYIGLDQNNTIICTDTESEISILATIEPLAG
                     FNEEISITVDSLPAGVTANISPQTTTLPANVAITLGNLTQLQEGSYSIFINIKSNTLL
                     KTVKYSFLISKGAPGKVVLQNVAADSKNISVKPQLSWDAVPGTKTYHVVVAKDYAFTD
                     KIFESDTKNTNISVQGLEFHTVYFWKVNAKNECGDGSDSDIRAFQTINQICNEYVQKT
                     PVIIPDNKVFDGNSTIHITDSFNISELSVSVKAQHTYLVDLKLDLLSPNNKTITLLGN
                     VCPGAQDVDAVFSDKGVPISCNAASPAVSGLVKPSVGALSEYEGNGVSGTWTLKISDN
                     YPQDGGQLQSWNIRACTIDSTTKTPLLTNNNTLYLLENTSSKIDQSYLQTVDTSLTPA
                     NIRYFITKTTQNGQLRKGLNVLKIGDWFTQTDINNKLISYKNTSFDDGLDGFGFYVLD
                     INGNWKPEQQFNIVIFKALKAEGNANHIECHGEKDGTITIHAENGIPPYEYSLNGSIF
                     ISDNTFTDLAAGSYDYQVKDLTNTIIDGTVTVTEPDSLILDIIKAGYNLTLSISGGVP
                     PFLASIDGVNFSETLNFTNLSMGNYLVSVKDANDCVVSDTVSILTSVNNVDHAGLSIF
                     PLPADAKLNIQLKNIQVRNISITDVMGRRQQENLHKYTISDKILINTSSLPSGMYILM
                     IETDDTLYSTKLIIQH"
     gene            complement(7402..>8005)
                     /locus_tag="PLE23_06670"
     CDS             complement(7402..>8005)
                     /locus_tag="PLE23_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82982.1"
                     /translation="LLTYAMILLMLIDPYLFGIQQFSDGIKFVLPVLISTVIIPAFAI
                     MMMKQLGMVKSIELDDKMDRTGPFIVTGIFYLWLFKNLYTNNTLPDLVTCFTLGSTIT
                     LFVCFIINIITKISIHAAAVSGFAVMVSIMCFHYFDSGRTINIGQVMLPLPLILFVVV
                     ILTGLVGTARLYLRAHSLAQVMSGYIVGLISMLFAVWYIL"
BASE COUNT         2426 a         1619 c         1602 g         2358 t
ORIGIN      
        1 ctcgccatat aaccgtccag ttcattcagg cggtgcagtt cggcttcatc ttttccgtac
       61 ttatgctcaa accacagata ttctgagtag ttggcaaaac cttcgttgag tgtgagattg
      121 gcccagcttt cgcatgtaac ataatcgcca aaccaatggt gcgatatctc gtgtgccagg
      181 atataatcat tcggattttc ttcgagctcg tcagcgtggc gttgtataaa ttctccgtaa
      241 atgatagctg tggtattttc cattgcaccg ctgacatagt cacgaaccac tatttgggat
      301 agtttaggcc acggatattt aagcccgaaa gtattggaga agaattcaag catttcgggt
      361 gtatgattgt aaatgagttt tgcatctttt tcatattccg gctctgtgta atattccaac
      421 ggtatatctt tccactggtc ttttactact gcgtattctc ctatcgccat cataaaaaga
      481 tatggtgcat gcgggaggtc catcttccag taatcggttc gggtaccgtc cgtatttacc
      541 ttgctgctaa ccaacagacc gtttgaaagg gttttgtatt ttttatctac tgttatgtaa
      601 agttcctgag tacacctttc atttggtcgg tcaatggtag ggaaccacct actgttgttt
      661 tctgcttcgc cctgtgtcca tatctgggtt ggtttatccg gatcttcgtt ttttggatca
      721 ataaaaaata accctttctc tgaagtaatg gcagcacttg atcctgccgg aaattcgctt
      781 gggcgagcta tatagtctat cactatagtc acttcatcgt taggcgtata tgtcttgtca
      841 agattgatgt aaagattggt atcatcataa ttgtaggtca agttcgtatt gccaattttt
      901 actgaattga taataaaatt cttagcatca agtctgaatc tgttgatatc atatccatag
      961 ggtttgaata ctaacgcagc ctttcctaaa acctgttctt tggcccagtc aaatgacact
     1021 tcaagtttgg tgtgtagtaa gtctatttct cgcgaatagg agggcttata ttccggtaat
     1081 ggctcaatcg tgctgtcttc tgcagctact gcttcttctt ggtcttccgg tacgacttct
     1141 agattatcca gattaactat ttcgtccgga ataatagctt gagtagcttt tttggaggtc
     1201 gaacaagaga gcaaaactaa ggcaaagact agaacgatga agcgcatggt gatgttttta
     1261 attttatgaa aaagtaaatt tgaaaaaatg tttacaataa acgtaattta taacataatt
     1321 aatgtcagta tagcagttta tttaatctgt ctgattaaat ttgtctgaaa tacggtttat
     1381 caataagctg atatctgtat cataagcaca cttgaagtgt ttttcggggc atgcttggta
     1441 accatggaga ccacaaggcc tgcaataaag cccactttca acctgtacaa ccactgagtc
     1501 atctgccaac ggaccaaaac caaactctgg aatggtagaa caataaacag cagtggttgg
     1561 tgcattaact gcagagcaca aatgcattgg tgccgaatcg ttgacatagt tcataatggc
     1621 atctttcatc aaagcagctg attgtagcaa acttaatctg ccggcaagat tgaccgatag
     1681 ctgatggctt gcagcagcaa taattctgtc acataatgct gtatcagccg gagccccgag
     1741 aaaatatacc accacattac tgttgagctg cccgacaaat tcggcccacc tttcctctgg
     1801 aaattgtttg gtgaaccaaa gagatgcagg tgaaatggta atataaggca tctgtttgta
     1861 ggttaagaca gatgcataca cactactgtc cggataaagt cttggcattt tgtttgaacg
     1921 gtctgttata ctttctatca gttggaggtt acgttctatc tcgtgttttg aaccagtgcc
     1981 gatctggtgt ttgattctta cattaaacaa taccgaaagt ggatttttat caaagcctaa
     2041 tttcagaggc gctcctgata atgcagtaat aaaaccggat gaagcaaaac gctgtatatt
     2101 gattacacaa tcatattgtt cagaccttat tattttaatt agtttgagca gattttggta
     2161 tttcgctctc cttttgtccc agactaaaat cttgcgtata aaaggatgac gatcaaacaa
     2221 tggttcatta ccttttttca caagcatgtc aatggatgca tgcggataaa acgagtgcag
     2281 tttttcagca acaggcgtcg ccagaatgac atctccaatg gatgctgtct gaataatgag
     2341 aaatctacct aggctatgtt gtgttgtcgg cactttgtct tatttaaact gccacattgt
     2401 attctcttaa tacgtcattg agtgaagttt tcaagtcggt agaaggtttc cgttggccaa
     2461 tgattaatgc acagggtacg tggtaggtac ctgcaggaaa agttttaggg tacgagcccg
     2521 gaataactac tgagcgctcc ggtactattc ctttaaaaac aacgggttcg cttcctgtta
     2581 catcgataac gtgggtggaa gctgtcagta ctacattggc accgagcact gcttcctttt
     2641 ctatcagcac accttctaca acaatgcatc tggagccaat aaaacaacca tcttcaataa
     2701 taacaggtga agcctggaca ggctcgagca cgcctcctat gcctacgccg ccactcaggt
     2761 gtacattgcg ccctatctga gcacaagagc ctactgtagc ccaagtgtca accatagtac
     2821 cggtacccac gtatgcacca atattgacat aagagggcat cattataaca ccagattcga
     2881 gatgagcacc ataacgtgca atggcatgtg gcactaccct tacaccttga gctgcatagt
     2941 cctttttaag tggaattttg tcatgaaatt ccattggtcc ggcttcaaag gtttccattt
     3001 tacgaatagg aaaatatagt aacaccgctt tttttatcca ttcattcacg acccagcctt
     3061 ttgaatcggt tttttcggca attctttttt ttcccttatc aagcaactct attacctgtt
     3121 ctattgatga aatggtgtcg ggttgtgaaa gcaaagtgcg gtcttgccat gcactttcaa
     3181 taatgtcctg cagtttattc atttctcttt tttaatttca ggcgtaaaat taacacaact
     3241 aatgatatga gccgatgttc gtaaatgcaa acattgtgtt attaatgatt tttatagcat
     3301 caataataat tttatgaagg tttcagcatt aagctgatga taagggctta cttttgagat
     3361 ttgatcaaac aaaacaatta atatttcaac agccatgacg ggaatcaaaa aaacgcttct
     3421 atactgcttt attgctctca tttcaataac aaatgccgaa gcccaatgga aacaaattaa
     3481 gaattcgcaa acaattacca atgtaggaaa aactgttgcc acagtcgaaa aggcagcaat
     3541 tttttcactc gacaaacaat ttctgaatga aataagtatg ctaaaattat ctggtgacag
     3601 caaaaatgat ggcgaatcaa aaataatatc gctgccattg ccagatggaa aatactacac
     3661 tgtaagcctt catcaaacag ccttatttca tccaggtttg cttaaaaaat atcccggtat
     3721 ccagtcattc acgggctatg gtattggaga tgcattttca ctggtcaagt gtagtatagg
     3781 agaagatggt tttcacggaa tgataatttc acccaaatac ggcaatattt tcatagaccc
     3841 attgactgat gccacatcat ctgactatat tgtttacaat aaagttgatt attctataga
     3901 cggaaaaaat gggtttgaat gtacattatc acatgcaaat acaggcaggc cttacagtca
     3961 agtagatgcc taccacgctc aaaaaccaga ttgtacattc agaaaatatc gtctggccct
     4021 tgcttgtacc ggcgagtatg ctgcctttca tggaggtact atagctaagg tattgtctgc
     4081 catgaacact accatgaccc gcgtaagtgg agtatatgag cgagaccttg cagtaagctt
     4141 cgagatcata ccagataacg acaaactggt attcctgaat ggacagaccg atccttattc
     4201 gaataataat ggtggtgcaa tgctctacca aaaccaacag actgttgata acctcatcgg
     4261 tgaagataat tatgatatag gccacgtatt cagtaccggt ggtggcggca ttgcttcact
     4321 ccagtcggtt tgcaatagtg gattcaaagc acagggagta accggttcgt cagcacccgt
     4381 tggtgaccct tttgacattg actatgtatg tcatgagatg ggtcatcagt ttaatgctta
     4441 tcatacattt aacaatgctt gcggtggcaa tattgctgtt gagacagctt ttgagcctgg
     4501 atcgggtact accatcatgg gttatgcagg agtgtgtgac ccaaatgttc agtttaattc
     4561 tgatgcttat ttccattcta ttagtctcag ccagatatca acttatatta ccaaaggatt
     4621 tggtagtgtt tgccctgtaa aaaccaactt aaacaatact gcacctactg taagtgccgg
     4681 tggtgataaa ataataccaa aaagtacagc ttttgagctt acagccactg cttcagatgc
     4741 cgatggtgat tctcttacat attgctggga gcaaatgaac aacgaactgg tcattcatcc
     4801 gcctctgagc acttattcaa acggaccttc tttccgttcg ttttacccaa ctgcaagccc
     4861 tacaagggta tttcctcaga ttacaactat tatcaacaac agtacaagca aatgggaggt
     4921 actgcctgct gtagcaagaa atctggactt cactgtaact gtcagagaca atcataatgg
     4981 tggaggatgt tttggtaccg atgagatgag cataaaagta gatggaagct caggcccatt
     5041 tttggtaacg aaaccgaata ctgctttggt gtggacgatt ggcaatacag aaaatataac
     5101 ctgggatgta gccggtactg accaagtacc tgtcaattgc tttgccgtcg atatttatct
     5161 gtccgttgat ggtggatata cctatccgta ccttcttgca tcaaatgtac ctaataccgg
     5221 aaattacaca gtgcaggtac caaattatcc gggaaccaaa aacagaataa aagtaaaggg
     5281 atatggaaat gtgttttttg atatctccaa taccaatttc accatttcac aaccaaattc
     5341 tccttcagtt tatatcggtc ttgatcaaaa taatactata atctgtaccg ataccgagtc
     5401 agaaataagc attttggcca ctattgagcc tttggccggc tttaatgaag aaataagtat
     5461 tacggttgat agtttacctg ctggtgtaac agcaaatatt tcaccacaaa ccacgactct
     5521 gccggcaaat gttgctatta cacttggcaa tcttacacaa ttgcaggaag gaagctacag
     5581 tatattcata aacatcaaat cgaatacttt attaaaaaca gtaaaatact cttttctgat
     5641 aagcaaaggt gcacctggta aagtagtctt acagaatgta gccgctgaca gcaagaatat
     5701 atctgtaaaa ccacaactga gctgggatgc cgttccagga acgaagactt accatgtcgt
     5761 tgtagcaaaa gattatgctt ttactgataa aatatttgaa tctgatacca aaaatacgaa
     5821 cataagcgta cagggtcttg aatttcacac agtctatttc tggaaagtga atgctaagaa
     5881 cgaatgcggc gatgggtccg attcagatat cagggcattc cagaccatca atcagatttg
     5941 taatgaatat gtccaaaaaa ctccggttat cattcctgat aataaggttt ttgacggcaa
     6001 ttcaaccata cacattacgg acagtttcaa tatctcagaa ctaagtgtaa gcgtaaaagc
     6061 tcaacacaca tacctggttg atttaaaact agacctgctt tcaccaaaca acaaaaccat
     6121 tactctgctg ggcaatgtat gtccgggggc acaggatgtt gatgctgttt tttcagataa
     6181 aggcgtgccg atcagctgca atgctgccag tcctgctgta tccggtcttg taaaaccatc
     6241 tgttggtgct ttaagcgaat acgaagggaa cggtgtctcg ggtacctgga ctctgaaaat
     6301 ttctgataat tacccgcaag atggtgggca gcttcagtca tggaacattc gtgcgtgtac
     6361 aattgattcc actacaaaaa ctccattgct gacaaacaat aataccctct acttacttga
     6421 aaatacgtct tcaaagattg accaaagtta tctccaaact gtggatacaa gcctgactcc
     6481 tgccaatatc aggtatttca ttacaaaaac tactcaaaac gggcagctga gaaaaggtct
     6541 gaatgtatta aaaattggtg actggtttac ccaaacggat attaataata aactgattag
     6601 ttataaaaat acttcttttg atgacggact tgacggattt ggcttttatg tgctggatat
     6661 aaacggcaat tggaagccag aacagcagtt caatattgta atattcaaag cacttaaggc
     6721 cgaaggcaat gctaatcata ttgaatgtca tggtgaaaag gatggtacta taaccattca
     6781 tgccgaaaat ggtattccac cctatgaata cagcctcaat ggttcaatat ttatatctga
     6841 caacacattt acagaccttg cggcaggcag ctacgattac caggtcaaag atcttacaaa
     6901 tacaattatt gacggaacag ttactgttac agaaccggat agcctgatac ttgacatcat
     6961 caaggccgga tacaatctca cgcttagtat aagcggtggt gttccgccat tcctggccag
     7021 tattgacgga gtaaattttt ctgaaacttt gaattttacc aatcttagta tgggcaatta
     7081 tctggtgtct gttaaagatg ccaatgattg tgttgtgagt gatactgtaa gtattctcac
     7141 ttcagtcaac aatgtagatc atgctggttt gtcgattttc ccgttaccgg cagatgctaa
     7201 actgaatata caattaaaga atatacaagt tcggaatatt tctatcacag atgtgatggg
     7261 aaggcggcag caagagaatt tacacaagta cactatctct gataaaatat tgataaatac
     7321 ttcatctcta ccgtccggca tgtacatact tatgattgaa acagacgata ccttgtattc
     7381 gaccaagctg ataatacagc attaaagaat gtaccagaca gcgaaaagca ttgaaataag
     7441 gcccacaata taccctgaca taacctgtgc taaactgtgt gctctgagat acagcctggc
     7501 tgtaccgacc aatcctgtca gtattactac cacaaagagt atcaatggca gaggcagcat
     7561 cacctggcct atattgatgg tacggccgga gtcaaagtaa tgaaagcaca tgatcgagac
     7621 cataacggca aaacccgaaa cggctgccgc gtgaatgctt atctttgtaa taatattaat
     7681 gataaagcat acgaaaagcg taatggtgga gccaagcgta aaacaggtaa ccagatctgg
     7741 tagggtattg ttggtataaa gatttttaaa taaccacagg tagaatatac cggtaactat
     7801 aaacgggcca gtacggtcca ttttatcgtc aagctctatt gatttgacca taccaagctg
     7861 tttcatcatc atgatggcga atgccggtat aatgaccgtc gatattaaca caggtagcac
     7921 aaattttatt ccgtctgaaa actgttgtat gccgaacaaa taagggtcaa tcaacatcag
     7981 cagaatcata gcgtatgtta gcaaa
//
LOCUS       DAOQQY010000111         7888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3626_length_7888_cov_5.819482, whole genome shotgun sequence.
ACCESSION   DAOQQY010000111 DAOQQY010000000
VERSION     DAOQQY010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3626_length_7888_cov_5.819482"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..572
                     /locus_tag="PLE23_06675"
     CDS             27..572
                     /locus_tag="PLE23_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HMX82983.1"
                     /translation="MKTYFSGMFVIAMMAVASFAFCNNPIKGGQTAASKVKWYTWAEA
                     MELRKQNPKPIMVDLYTDWCGWCKRMDATTFEDEKVAKYLNDNFYPVKFDAEQKGEIT
                     YGGKVHKFVASGRNGYHELAAAMVDGQLGYPCFVYFNENMERIMFSPGYKGADDVMKE
                     LMFAAEGKYKTTSWDAYKAAK"
     gene            797..1543
                     /locus_tag="PLE23_06680"
     CDS             797..1543
                     /locus_tag="PLE23_06680"
                     /inference="COORDINATES: protein motif:HMM:NF020134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HMX82984.1"
                     /translation="MADFRTKITGTGSYIPELVKTNRDFTIHDFYDEKHVKIDSPPDV
                     VVRKFYEITGIEERRYASENLNNSDLGYFAAKKAIENSKINPEIIDQIIVAHNYGDVC
                     KHTIQCDTVPSLASRIKHHLGIKNPACIAHDVLFGCPGWLQGVIHADAFFKSGLAKTA
                     LVIGTETLSRVIDVYDRDSMIFSDGAGAVILQYLPSDDHSGIMSVCAQSHCVDEMGYI
                     HMGKSYFPQSNPRIRYIKMKGRKVYEYAMK"
     gene            1556..1873
                     /locus_tag="PLE23_06685"
     CDS             1556..1873
                     /locus_tag="PLE23_06685"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HMX82985.1"
                     /translation="MKECIDKAGIGIEQITKIFLHQANEKMDEAITKALYRLYGITDI
                     PADIMPMSINKLGNSSVATIPTLFDLVKNRLLGNHELHTGDIVLFASVGAGMNINAVC
                     YKI"
     gene            1891..2565
                     /locus_tag="PLE23_06690"
     CDS             1891..2565
                     /locus_tag="PLE23_06690"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HMX82986.1"
                     /translation="MSEYHSVIQMLQQHADEHNVQSMSRFGINTKNALGISIVTLRKM
                     VKPYKKNHELAVKLWASGIHEARLFAVLIDEPEKVTEQQFDQWSNEVDSWDLCDQMCS
                     NLFSKSVFAEKKIMEYAGSEHEFVKRTAFALIACFALKRMNKSDEFLLRYLPVIEQAS
                     DDPRNFVRKAVNWALRQIGKKNPILLEAATITANRILEQNTKPARWIANDALREFAKK
                     STFNPT"
     gene            complement(2562..3944)
                     /locus_tag="PLE23_06695"
     CDS             complement(2562..3944)
                     /locus_tag="PLE23_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82987.1"
                     /translation="MKSLDKKYQTQLERIAAEIQNSDTLESFLNDESEELFKELQIRF
                     EPAIAAVYSEVANEQPLQIIPLEKALLKDDFEGLFIPKVLAYSVLRGEVNARCKFVRQ
                     QDHMRDIIQYMLSSPNFEYIRKRSGQTIQMGFALSSDIYITNLIESVENKKLQQYLIA
                     QKQERLRDQDERIASLKKYRNQFKHDVYFTADFPNNLNELKVYYNSLRDFLHVRFARR
                     LDNSSLKDSLLAFLHNPEFTNELEFVTILGYTVNFIQYDKKEAEEVAGIFNHARKSFP
                     DFNKHYFEFVNYCLHSEIDLDAEADNRVMSFIDQSIKDDISSYYNLVNTIHTKGYIHE
                     DTVDAVKNFYEHHAGQSIVNECVRMVIYNYFEKLLTHLEPSDYQTFFDLSKVYQTYFD
                     IFSNEHFTQAVKHLSLDFTNKLLKVFTDKRGKDYQEVKRFVSATFVDFGFLKEKEVVE
                     MFKTRKKVVS"
     gene            complement(4420..4665)
                     /gene="rpsP"
                     /locus_tag="PLE23_06700"
                     /pseudo
     CDS             complement(4420..4665)
                     /gene="rpsP"
                     /locus_tag="PLE23_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002466054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
     gene            complement(4899..5432)
                     /locus_tag="PLE23_06705"
     CDS             complement(4899..5432)
                     /locus_tag="PLE23_06705"
                     /inference="COORDINATES: protein motif:HMM:NF039704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6252 family protein"
                     /protein_id="HMX82988.1"
                     /translation="MTKKIFSGKFGMICFGMLVSVLMLSSCKKDDTGGPSGSAAEGTI
                     QASVNGKTVTSLKIATFANRISGNLAIQGNTGGTSSEAFSLLITSENGVGTYPIGGGA
                     NIANIAAYIKTEIDLSNPGSPKTETWQAPYDATQAGEIKITEISTTNVKGTFYFKAKR
                     SDGNIVDVTNGSFNINF"
     gene            complement(5665..>7888)
                     /locus_tag="PLE23_06710"
     CDS             complement(5665..>7888)
                     /locus_tag="PLE23_06710"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX82989.1"
                     /translation="NAKTLYSFTQNLNSCNEGTVYRSWQVSDAGNNTAVCVQSIFVTN
                     KHDWNASNDKIFWPKDYTTNECKTTAELKPSFLPQGFSQPAIKDNSKCTNVEIKYEDT
                     NIGQGSVGCVEIHRKWSIIEKCTYAATNGTAGKWEHTQIVKVTDNIPPSLNVPADVTV
                     FITDNSCSAFVDLPVITVTDCDPNPIITNNKTNKPTNLSATYQAGVNKVEVTATDHCG
                     NTISRAVTITVLDGMPPQVLCKQNVHINLENLDATGNMELIPEMISNGFTDNCSPDFM
                     IKTSVFPTNISCSDLGKTNITLTVSDLSGNTAFCVSEITITDANQLCSKPLYRISGNI
                     RTADNKPVLAANVALNWGNTAIIKTKDGSFLFDKLTQGGNFDLSATKDTFVLNGISTY
                     DLLLLNEHILGKKILDSPYKYIAADVNQNNYITTADYVILKDLLLLNIDSLPGWKSWR
                     FVPDTFQFVNYHPILPLFPEKISVKNLQHDVTDANFVGVKLGDLNFSNNPSKVVDVDV
                     RGEESVEIEISGIETSVANEIRVPFVLRSDKPLKGIQFELMLADMDVDKYTMLLPAQM
                     KCNRQDVNFISTGNGTKMRLSWISTDDIQLVEGDTLLYLVLKNLSGNDFLEKIQLTMN
                     AIQPEAYDADYKIHKLMIREIANESVSTQKGNMLIFPNPVKDYFQIHFEYKYAEQADI
                     ALIDLSGRTILGQSVALKSGNNQFRFSHIDKMSSGLYLLQLKTENGRIFHQKIFVERH
                     "
BASE COUNT         2382 a         1548 c         1475 g         2483 t
ORIGIN      
        1 attttattaa aaaataaaac aaatacatga agacatattt ttcaggaatg ttcgtaatag
       61 ccatgatggc agtagcttct tttgcatttt gcaataaccc aatcaaaggt ggacaaaccg
      121 cagcttcaaa agtaaaatgg tacacgtggg cagaagcaat ggaattgcgc aagcagaatc
      181 ccaaacctat catggttgac ctttatacag attggtgcgg ctggtgtaaa agaatggatg
      241 ctactacttt tgaagacgaa aaggtagcaa aatatctgaa tgataatttt tatccggtaa
      301 aatttgacgc tgagcaaaaa ggcgaaatca catacggtgg aaaggtacat aaatttgtag
      361 cctcaggtcg taacggatac cacgaactcg ctgctgccat ggttgacgga caattgggtt
      421 atccgtgttt tgtttatttc aatgaaaata tggagcgaat tatgttttca ccgggatata
      481 aaggagcaga cgacgtcatg aaagaactaa tgtttgctgc cgaaggtaaa tataaaacca
      541 cttcatggga tgcgtataag gctgcgaaat aatatacagt caaatacgct gtagcgccca
      601 ttacaccatt aactcgattg tatctggccg attagttgta gctgttacta cataaagctt
      661 atattcctgc tctgactgca tttcagtttt cgtattggca tacaaaatgt actaaaacac
      721 attttttggt aattaaaatt tacatttaaa aacaagtaaa ttaggtgatt catctaaaga
      781 ccattacctt agtggcatgg cagactttag aaccaaaata acaggtacag gcagttatat
      841 acctgagctt gtgaaaacaa accgcgactt tacaatacat gatttttatg atgaaaaaca
      901 tgtcaaaatt gattcaccac ctgatgtagt tgttagaaaa ttctatgaaa tcacaggtat
      961 tgaagaacga agatatgcga gtgaaaattt gaataattct gatcttggct attttgcagc
     1021 aaaaaaagct attgaaaaca gcaaaatcaa ccctgaaata atagatcaga tcatcgtagc
     1081 gcacaactat ggtgatgtct gcaaacatac aatacaatgt gatactgtac cttctttggc
     1141 atccagaatc aagcatcacc tcggcatcaa aaacccggct tgcattgcac atgatgtatt
     1201 gtttggttgt ccgggctggt tacagggagt cattcatgct gatgctttct ttaagtctgg
     1261 cctcgccaaa accgcgcttg taattggtac agagacactt tccagagtaa tagatgtgta
     1321 tgaccgcgac agtatgatat tcagtgacgg agcaggtgca gttatacttc aatacttgcc
     1381 tagtgatgac cattcaggaa tcatgtcggt gtgtgcgcaa agccattgcg ttgatgaaat
     1441 gggatacatc cacatgggca aatcttattt tccacagtct aacccaagga taagatacat
     1501 caaaatgaaa ggtagaaagg tatatgaata cgctatgaaa taagtaccgc ttgccatgaa
     1561 ggaatgtatt gacaaagcag gcattggtat agaacaaatc actaagattt tcctgcacca
     1621 ggcaaatgaa aaaatggatg aagcaatcac caaagccctc taccgtctgt acggaatcac
     1681 agatatacct gccgatatca tgccaatgag cattaacaaa ctcggcaaca gctcggttgc
     1741 taccatccca accttgtttg atcttgtcaa aaaccgacta ctcggcaacc atgaattgca
     1801 tacaggtgat attgttctgt ttgcttctgt aggtgcaggc atgaatatca atgcagtatg
     1861 ttataaaata taaaaattct acacaccctt atgtcagaat accattcagt aatacaaatg
     1921 ctacaacaac atgccgacga acataatgtg caaagcatga gcagattcgg cattaatacc
     1981 aagaatgcat tgggcatttc tatcgtcaca ttgcgtaaaa tggtaaaacc atacaagaag
     2041 aatcatgaac tcgcagtaaa gctatgggca tcaggcatac acgaagccag actatttgct
     2101 gtcttgattg acgagcctga aaaagtgacc gaacaacagt ttgaccaatg gagtaacgaa
     2161 gtagattcgt gggatttatg tgaccagatg tgttctaatc ttttcagtaa gagtgttttt
     2221 gcggagaaaa aaataatgga atatgcaggt tctgaacatg aattcgtaaa acggacagca
     2281 tttgctttga tagcctgttt tgcattaaaa cgcatgaata aatcggatga atttctattg
     2341 cgttatttgc cggtgattga gcaggcatct gatgatccgc gcaattttgt aagaaaagct
     2401 gtcaactggg cgctcaggca aataggaaaa aagaatccta ttcttttgga agcagctact
     2461 attactgcga atagaatatt ggagcagaat actaagcctg cacgctggat tgccaatgat
     2521 gccctacggg aatttgcaaa aaaatctact ttcaacccaa cttaagacac aactttcttt
     2581 ctggttttga acatttcaac cacttccttt tctttcaaaa atccaaagtc cacgaatgta
     2641 gctgaaacaa aacgcttcac ttcctgataa tctttgccac gcttatctgt aaatactttt
     2701 aacaatttat tggtaaaatc cagtgaaagg tgtttgacgg cctgtgtaaa atgctcattt
     2761 gagaatatat cgaagtacgt ttgataaact ttggataaat caaagaacgt ctgatagtca
     2821 gatggttcaa gatgtgtaag taatttctca aaataattat aaataaccat tctcacacat
     2881 tcattcacga ttgactgacc ggcatggtgc tcgtagaaat ttttgacagc atctacagta
     2941 tcttcgtgga tatatccctt ggtatggata gtatttacaa gattgtagta gcttgaaata
     3001 tcatctttga tgctttggtc aatgaaagac atgacccgat tatctgcttc agcatccaga
     3061 tctatctcag aatgaagaca ataattgaca aattcaaaat aatgcttatt aaaatcaggg
     3121 aatgatttcc gtgcatgatt aaaaataccg gctacttctt cagcttcttt cttatcgtat
     3181 tggatgaaat tcacagtgta gccaagaata gtaacaaact ctagctcatt ggtaaattca
     3241 ggattgtgca aaaatgcaag caaactatct ttcagactag agttatcaag tcgtcttgca
     3301 aaacgaacat gtagaaaatc acgcaaacta ttgtaatata ctttaagctc gttcagatta
     3361 ttcggaaaat cggcagtgaa gtacacatcg tgtttaaact gattgcggta tttcttcaag
     3421 cttgctattc tctcatcttg gtcacgtagt ctttcttgtt tttgagcaat aaggtactgt
     3481 tgaagctttt tgttttcaac actttcaatc agattggtaa tataaatatc acttgacagc
     3541 gcaaaaccca tttgaatggt ttggcctgag cgcttccgga tatactcgaa gttaggtgat
     3601 gacagcatat attggatgat atcacgcatg tgatcctgct gtcttacaaa tttacatcga
     3661 gcatttactt cgccacggag tacagaatat gccaatacct ttggaataaa aagcccttca
     3721 aaatcatctt ttagtaaggc tttttccaga ggaataatct ggagtggttg ttcattggcc
     3781 acttcactat acacggcagc tatagcaggt tcaaacctta tctgaagttc tttaaacaac
     3841 tcttcgcttt catcatttag aaaactctcg agtgtatcgg agttttgaat ttcagcagct
     3901 attctttcga gctgtgtctg gtatttttta tcaagtgact tcattgcgtt tatgatttaa
     3961 aattgaaacc taacaagcaa tgaaggcctg ttaggtttct gaaaaaatta attttatttc
     4021 tgctgcaaat atacaaaatg tagatttata aaaataaaaa gcgaggtaaa actttacctc
     4081 gccttttaaa aaaccatatt tatcgaatat aaattattct gttacctcag gtgcctggtc
     4141 agccgatgat tcttcagctg caggtggtgc agtatccgct gttggttcct caagcggtgt
     4201 ttctgttact tctgctgtcg tttcctctac aacaggttct tcaactttct ttggcttagc
     4261 agtacctgat accaactggt ggaatctcga aacttcctct ttcttacttt caatacgttt
     4321 agcaactttt tctgatttgg cgtcaagcca tgttttgtgt ttttcttcag cttgctcaag
     4381 cgttagagcg cctttgcgta ctccgcgcat taggtgtttt ttgtacatta ctcccttgaa
     4441 gccaagaata gcacgcactg tatctgttgt ctgtgcacct ttctcaagcc actcataagc
     4501 tgcctctgca tctaggttga tgatagcagg tgttgtcatt ggattgtaag taccgatttt
     4561 tttgataaat ttaccgtctc ttggtgatcg tgagtcggct acaacgatgt ggtagaaagg
     4621 tgctttctta cggccctttc tctgtagtct gattttaaca gccatttttt ttgttttatt
     4681 gttaaaaatg aatccaacta accttgatta acaagcggtt gaattttgcc ggtgcaaagg
     4741 tataattatt atatattata aacaaatttt atttcggtat atttctactt tcctgaataa
     4801 ctaaacaaac ctacgtcttc aaaacaaaaa tggcatcgcc cggactaccc gacaatacca
     4861 tacttgggat tctttttgct agatgcttat ctgagaaatc agaaattgat gttaaatgag
     4921 ccattggtaa catcaacaat atttccgtca cttctttttg ccttaaaata aaatgtgcct
     4981 tttacgttgg tggtcgatat ttctgttatc ttgatttcgc ccgcttgtgt ggcatcataa
     5041 ggtgcctgcc atgtttctgt cttcggcgaa cccggattag aaagatctat ctcagtcttg
     5101 atatatgctg cgatgtttgc tatgttagca ccgccaccta tcgggtaggt tcctacgcca
     5161 ttttcactgg tgatgagcag tgaaaaagcc tcacttgatg ttccaccggt atttccctgg
     5221 atagccaagt tacctgatat tcggttagca aatgtagcaa tcttcaatga tgttaccgtc
     5281 ttgccattga cactcgcctg aattgtacct tctgctgccg aacccgaagg tccaccggtg
     5341 tcatctttct tgcacgaact cagcatcaac acagatacca acattccgaa acaaatcatc
     5401 ccgaattttc ctgaaaaaat cttctttgtc atagtcttaa ttctttttag ggttaactta
     5461 atatatattc aatttcatat tacgtataaa tttatatgtt gtttctctat aaatccttca
     5521 acaatattct ttatatacag ttttcattag aatttcatca aaaaaactaa ctaatcattt
     5581 cctcaaacaa tactatattg aaaaaaaggt tcctctcaaa acaaaagagg aaccattacc
     5641 atgagaaaca taaaaacctg attcttagtg cctttcgaca aatatctttt ggtggaatat
     5701 tcttccattt tcagttttca attgcaataa atataaccca ctgctcattt tatcaatatg
     5761 tgaaaatctg aattggttat tacctgattt cagtgccaca ctttgaccta atatcgttct
     5821 gccgctgagg tctatcagag caatatcggc ttgttctgca tatttgtatt caaagtgaat
     5881 ctgaaaataa tctttaacag gatttggaaa aatgagcata ttgcctttct gagtgcttac
     5941 tgattcgtta gcaatttctc ttatcatcaa tttatgaatt ttgtaatccg catcataagc
     6001 ttcaggttga attgcattca ttgtcagctg aattttctcc aaaaaatcat ttcctgaaag
     6061 atttttcaac actagataca acagtgtatc accttctacc agctgaatat catcggtcga
     6121 tatccagctc agcctcattt ttgtaccatt acccgtgctt ataaaattaa catcctgtcg
     6181 gttacatttc atttgagcag gaagcaacat agtgtactta tcaacatcca tatctgcaag
     6241 catcaactca aactggattc ccttcaatgg tttgtcgctg cgtaatacga atggaacacg
     6301 tatttcattt gctacagaag tttcaatacc ggaaatctct atttcaaccg attcttcacc
     6361 tcgaacatct acatctacaa cttttgaagg gttgttgcta aaattaaggt caccgagttt
     6421 tactccgaca aaattggcat ccgtcacatc gtgctgaaga ttctttactg aaattttctc
     6481 cggaaataat ggtaaaatcg gatgataatt aacaaattgg aaggtatcag gtacgaaacg
     6541 ccacgatttc catccgggca aagaatcaat attcagcaac aaaagatcct tcaatataac
     6601 ataatcagcg gtagtgatgt aattgttctg attaacatcg gctgcaatat atttataagg
     6661 cgaatcaagg attttcttgc caagtatatg ttcatttaac aacagaaggt cgtaggtaga
     6721 aataccgttg agcacaaacg tgtctttcgt tgcagacaaa tcgaaattgc ctccttgagt
     6781 gagcttgtca aagagaaaac taccgtcctt cgtttttata atagcagtat tgccccaatt
     6841 taacgcaaca tttgcggcca ataccggctt gttatcagct gtacgaatgt tgcctgatat
     6901 tcgatacagc ggcttgctac acaattgatt tgcatccgta atggtaattt cgctcacaca
     6961 gaatgctgta ttgcccgaca agtcagaaac cgtcagcgtt atattcgttt taccaagatc
     7021 gctgcaggaa atattggtcg gaaacacgct cgtctttatc ataaaatccg gcgaacaatt
     7081 gtcggtaaaa ccattggata tcatttctgg tattagctcc atattgccgg tggcatcaag
     7141 attttcaagg ttaatatgta cattttgctt acaaagtact tgtggcggca taccgtccag
     7201 gactgtaata gtgacggcac ggctaatggt attcccacaa tgatcggttg ctgtcacttc
     7261 tactttattt acaccggctt ggtaggttgc agaaagatta gttggtttgt ttgttttgtt
     7321 gttggtaatt ataggattgg gatcacaatc agttacagta attacaggca aatcaacaaa
     7381 tgccgaacag ctattgtccg taatgaagac cgtaacatca gccggtacat tcaaagaagg
     7441 cggtatattg tcagtcacct taacaatctg tgtatgctcc cattttccgg ctgtaccgtt
     7501 ggtggctgcg tatgtgcact tttcaataat gctccacttg cggtggattt caacacatcc
     7561 aacacttccc tgcccaatgt tagtgtcctc gtattttatc tcaacattgg tacatttcga
     7621 attgtccttt atagcaggct gtgagaatcc ctgaggtaaa aatgatggct tgagctctgc
     7681 tgtcgtcttg cattcattgg tagtataatc ctttggccag aaaattttgt cgttgcttgc
     7741 attccagtcg tgtttgttgg tgacgaatat actttgcaca cataccgctg tgttatttcc
     7801 ggcatcgctt acttgccagc ttcggtacac tgtgccttca ttgcatgaat taagattttg
     7861 tgtaaaagaa tatagcgttt tggcattg
//
LOCUS       DAOQQY010000112         7808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3688_length_7808_cov_6.025539, whole genome shotgun sequence.
ACCESSION   DAOQQY010000112 DAOQQY010000000
VERSION     DAOQQY010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3688_length_7808_cov_6.025539"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..1081
                     /gene="xerD"
                     /locus_tag="PLE23_06715"
     CDS             164..1081
                     /gene="xerD"
                     /locus_tag="PLE23_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HMX82990.1"
                     /translation="MDWHSTLRGFLAYMTLEKGFSVHSKEAYIRDVKGLMDFVAEQHE
                     TQSPVDVRQEDVAAFMKYLYDMGLHVRTQARVLSGIKAYYKYMVVENIMQDSPAALIE
                     GPKLTRKIPDVLSVDEIQQMLDAIDMSEPNGQRNRAMIEVLYACGLRVSELTDLRISN
                     LFLEVGFVRVIGKNNKERLVPIGSEAIKQLTYYMDGVRSGMMNIKEDSQNVVFLNRNG
                     RKMTRVMVFYIIKELAVKAGIKKTVSPHTFRHSFATHLVEGGADLRAVQDMLGHESIT
                     TTEIYTHLDTDYLRDVVLRFHPMNRREFS"
     gene            1127..2755
                     /locus_tag="PLE23_06720"
     CDS             1127..2755
                     /locus_tag="PLE23_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HMX82991.1"
                     /translation="MTLRNKYLLNLLAGCIYALYMAACANPIAPEGGAKDTTPPRVDS
                     LRSSVSGRKNFTKEPVVFRFDEWVVLDNIGQQLLVSPPTVYAPKVVLKGKTVHFTFDN
                     REVLKENTTYSISLGEAVKDLTEGNVAKDVRFVFSTGDQIDSASLFSTIKLSLTKESV
                     KGAVAMLYEAADNDSVIIKRKPDYFARGGETGTFTIENIRPGRYKVFALKDDNLNYLY
                     DLDSEMIGFADSVLVIDKNEHKTITAMEVSEEAKPPRISDKRADRYGLVQCTFNGNPK
                     LVKTEIVTSGQEYIAETDKDTLKIWYKPEPDTDWEVHFDNGVTKDTVRVKPKSAKEDK
                     RRKTLLLLNQKAYFNKRIVETEPFRIDLTNPVSYFDTSRIMLMADSLPFKSGKYLELR
                     KDKPRAIFVNAPWEEGVHYKMTMLPGSATDMYGNKNDTIRADWRVLTGKDLGNISLNI
                     DNLDAEKAYIVQLLNAGGEVLEEFNVQNERKWEKLILNKQPGKYEVVIIVDLNRNGIW
                     DPVRLFPRRQAEPIFRKKPDALRANWELNVTISL"
     gene            2781..3518
                     /gene="lptB"
                     /locus_tag="PLE23_06725"
     CDS             2781..3518
                     /gene="lptB"
                     /locus_tag="PLE23_06725"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HMX82992.1"
                     /translation="MNILRATHLQKIYGPRTVVGDVSIEVKQGEIVGLLGPNGAGKTT
                     SFYMMVGFIKPNKGQVFLDDTEITDLPMYRRAQKGIGYLPQEASVFRKLTVEDNIMAV
                     LEFNVKTRTEQKARLEELIDEFSLHKVRKNMADTVSGGERRRTEIARALASNPKFILL
                     DEPFAGIDPIAVEDIQTIVAKLKDKNIGILITDHNVQETLSITDRAYLMFEGNILRAG
                     TAEQLAEDEVVRKVYLGKNFVLQKKEL"
     gene            3523..3777
                     /locus_tag="PLE23_06730"
     CDS             3523..3777
                     /locus_tag="PLE23_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82993.1"
                     /translation="MAGIKKLCLLSLILLGACKEQAAKISVEERLMIDTMSANKVARL
                     RADWIRDCDRKKDSMVQFAIDSLTAVTLEKINLKLKPYHE"
     gene            3770..4114
                     /locus_tag="PLE23_06735"
     CDS             3770..4114
                     /locus_tag="PLE23_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82994.1"
                     /translation="MSKKQIWLILLALATVYLVIKFRQERVINRSLVDAKVQEIVMFE
                     KAEYEKTCKKDALRQAAVVVDSLLQVWGEQSSFDTTGRPPLPFKPTAPDFQSPLDRVT
                     LKPLFDSIQSIK"
     gene            4375..5457
                     /locus_tag="PLE23_06740"
     CDS             4375..5457
                     /locus_tag="PLE23_06740"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMX82995.1"
                     /translation="MEKAKRIQFERKSSTDFYQVLSKRVEDYFKTNNISKHANGAMVF
                     KTIFVLTVFILSYIGIISNVFGLGMVLFLFAVNGFFAAMIGFNISHDAVHDAYSSNKT
                     LNRILGTTFNFLGTSDYVWKIKHNIVHHTYTNIPDHDSDIEDIPFIRLTEVQERKWNH
                     RFQHIYMFFFYLFTTLSWVFKNDYVNFFRRDWGWYKNPDHPKVEYFRLFFYKALYYFM
                     FLVLPFLVVDQPWYFILLGFVILHFVEGMTLALVFQLAHVVEEAQFPEPNDEGKLEDT
                     WAVHQLRTTANFARKSWLANFICGGLNFQIEHHLFPAICHIHYTKLAPIVKQTASEFG
                     LPYHEAKTFGGALMSHIRIMQRLGTA"
     gene            5740..6195
                     /locus_tag="PLE23_06745"
     CDS             5740..6195
                     /locus_tag="PLE23_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82996.1"
                     /translation="MRNKQMLMRISFGIIFFWFGALKFFPSVSPAEELASETIYRLTF
                     GIFSKHTGLILLAILECGLGLMFLSGKYTKATILTAIGHMLCTFTPFFLLPEASFTQA
                     PYAFTLVGQYIMKNIVFIVALTMMWPEAQTGVIRQQKNIKRIMHEDVAV"
     gene            complement(6333..6686)
                     /locus_tag="PLE23_06750"
     CDS             complement(6333..6686)
                     /locus_tag="PLE23_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HMX82997.1"
                     /translation="MSELNETTTTPVTITQSAIKQLEVIRREQNIPEDQGLRIGVKGG
                     GCSGFSYMIGFDHKQDSDQEFIINGMKVFMDKLHGIYLLGMEIDWDEGLNNRGFTFKN
                     PNAKTTCGCGTSFSA"
     gene            7061..>7808
                     /locus_tag="PLE23_06755"
     CDS             7061..>7808
                     /locus_tag="PLE23_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX82998.1"
                     /translation="MKNLILLISILLFSSGPYLHAQNSFSTDFESYNEGDWVAGNDPT
                     HWRTWSSETGGTSDDAKITSERAASGTKSFKIQNTVTGGGPEDLILKLGEAYTTGTVT
                     LGFKMFIEPGGRGYFNLQSGEQPGLFGLDFFFQEFGEVVGWSHQNYIYNTSSHPIGEW
                     FDCKMVVDPANNMWTLSINNQCIQVYRGNYASVSGVDFFANPGTNYFIDDVYYNYDPT
                     PVVYSGAEAGLINLNIISSTQIKGFPFSFSN"
BASE COUNT         2424 a         1466 c         1871 g         2047 t
ORIGIN      
        1 aagtaaaggt aagggataga agccggcacg aagtaaggcg gatagcccga cccgtagggg
       61 tacgcccaaa tgagtaaaca aaaggtggct tgcaaaatgt gaaagattaa gttgttgggg
      121 tgagcggtat ttgtgaaaaa atgttatctt cgaaaaaaaa agattggact ggcattcgac
      181 gttgcgtgga tttctggcat atatgacact tgagaagggt ttttcggtgc attcgaagga
      241 ggcttatata cgtgatgtga agggattgat ggattttgtg gctgagcaac acgagacgca
      301 gtcgccggtg gatgtgagac aagaagatgt ggcagcattt atgaaatatc tgtatgatat
      361 gggcttgcat gtgcgtacgc aggcgcgtgt gctgtcgggt atcaaggcgt actacaagta
      421 tatggtagtt gaaaacataa tgcaggacag tccagcggca ctgatagagg ggccgaaact
      481 gacgagaaag ataccggatg tactgagtgt ggatgaaata cagcagatgc tggatgccat
      541 agacatgagc gaacccaacg gccagagaaa ccgtgctatg atagaggtgt tgtatgcctg
      601 cggactgcgt gtgtcggagc taactgacct gagaatatcg aacttgtttt tggaggtagg
      661 tttcgtgcgg gtgattggca agaacaacaa ggagcggctg gtgccgatag gtagtgaggc
      721 gatcaaacag ctgacgtatt atatggatgg cgtgcgtagc gggatgatga atataaagga
      781 ggattcgcag aatgtcgtat tcctgaacag gaacgggcga aagatgacgc gtgtgatggt
      841 tttttacatc ataaaggagc tggctgtgaa ggctggtata aagaaaacgg tgagtccgca
      901 tacattcagg cattcgtttg ctacacacct ggtggaaggc ggcgcagacc ttcgggcggt
      961 gcaggatatg ctggggcatg agtcgataac cactacagaa atatacacgc acctggatac
     1021 agattatctg cgagatgtcg ttttaaggtt tcatcctatg aatcggcgag aattttctta
     1081 attttgggcc ttgaaataaa aactggactg aaaatacaaa cggattttga cattgcgtaa
     1141 taaatatttg ctgaatctgc tggcaggctg tatatatgcc ttgtatatgg ctgcatgtgc
     1201 caatccgata gcacctgaag gtggtgcaaa ggatacgaca ccaccaaggg ttgactcgtt
     1261 gcgttcttcg gtatcaggca ggaagaattt tacgaaggag cctgtggtat tcaggtttga
     1321 tgagtgggtg gtattggaca atatagggca gcagctactg gtgtcgccac cgacggtata
     1381 tgcgcccaaa gtggtgctaa aaggaaaaac cgtacatttc acctttgata accgtgaggt
     1441 gttgaaggaa aatactactt acagcatcag tctgggagag gcagtcaaag acctgacgga
     1501 aggcaatgtg gccaaggatg tacgttttgt attttcgacc ggtgaccaga tagacagtgc
     1561 gtctttgttt tcgaccatca agctaagcct gaccaaggaa agtgtgaagg gcgctgtggc
     1621 catgttgtac gaagcggcgg acaatgactc ggttatcatc aagcgcaagc ccgactattt
     1681 tgcaaggggc ggtgaaacgg gtacctttac catagaaaac ataaggcccg gccggtacaa
     1741 ggtgttcgca ttgaaagacg acaacctcaa ctatctgtat gatctggaca gcgaaatgat
     1801 tggatttgct gattcggtgc tggtgattga taaaaacgaa cacaagacta taactgccat
     1861 ggaggtatcg gaagaggcga agccacctag aataagcgat aaacgggcag accgctatgg
     1921 gctggtacag tgtactttca acggcaaccc gaaactggta aaaacagaaa tagtgacatc
     1981 tggccaggaa tatatagcag aaacagacaa ggatacactg aaaatatggt acaaaccaga
     2041 acccgatacc gactgggagg tacacttcga caatggtgta accaaggata ctgtacgagt
     2101 aaagcccaaa tcggccaagg aagacaaaag gcgaaaaacg ctgttgctgc tgaaccaaaa
     2161 ggcgtatttc aacaagagga ttgtagagac ggagccattt aggatagacc tgaccaaccc
     2221 tgtcagttac tttgacacat cgaggatcat gctcatggct gattcattgc cgttcaaaag
     2281 tggcaaatac ctggaactca ggaaagataa gccacgtgcc atattcgtca atgcaccatg
     2341 ggaagagggc gtacattata agatgaccat gctaccgggg agtgcaaccg atatgtatgg
     2401 caataagaat gacactatac gagctgactg gcgtgtactt accggcaaag acctgggcaa
     2461 tataagtctg aacattgaca atctggatgc tgaaaaagcc tacatcgtac aactgctgaa
     2521 tgcaggtggt gaggtattgg aggagttcaa tgtacagaat gaaagaaaat gggaaaagct
     2581 gatactgaac aagcaacccg gtaaatatga ggtagtcatc atagtcgatc tgaacagaaa
     2641 tggcatctgg gaccctgtgc gattgttccc gaggaggcag gcagaaccca tcttcagaaa
     2701 aaaacctgac gcattaagag ccaactggga acttaatgtc acgatatcct tgtaattttg
     2761 tgaaataatc agacaaataa atgaatatac tccgagccac acatctgcag aaaatatacg
     2821 ggccacgtac ggtagtgggc gatgtgtcaa tagaggtaaa acaaggcgag atagtggggc
     2881 tactcgggcc taacggtgcc ggcaagacta catcgttcta tatgatggta ggctttataa
     2941 agcccaacaa gggtcaggta tttctggatg acactgaaat aaccgacttg ccaatgtatc
     3001 ggcgggcgca aaaaggtata ggctatctgc cacaggaggc ttcggtattt cggaagctga
     3061 cagtggaaga caatataatg gcggtactag agttcaacgt aaagacacgt accgagcaga
     3121 aagcaagact tgaagagctg atagacgagt tcagcctgca caaggtaagg aaaaatatgg
     3181 cagataccgt atcgggaggt gaacgacggc gtaccgagat agcgcgtgca ctggcttcga
     3241 accccaagtt tatactactc gatgaaccat ttgcaggaat cgaccctata gctgtagaag
     3301 atatacagac gatagtggca aagttgaaag ataaaaatat aggcatactc atcacggacc
     3361 acaacgtgca ggagaccctc tctattaccg acagggcata tctgatgttc gagggcaata
     3421 tactgagggc aggcactgca gaacagctgg cagaagatga agtagtacgc aaagtgtatc
     3481 ttggaaaaaa tttcgtttta caaaaaaagg aactctgaca ggatggccgg tataaaaaaa
     3541 ctttgtctgc ttagtttgat actgcttggt gcctgcaagg aacaggcagc aaaaataagt
     3601 gtagaagaac gcctgatgat agacaccatg agtgccaata aggtagccag actgcgcgcc
     3661 gactggataa gagactgcga ccgcaagaag gattctatgg tgcaatttgc tattgattcg
     3721 cttacggctg tgacgctcga gaaaataaac ctcaaactaa agccctacca tgagtaagaa
     3781 acagatatgg ctgatattgc tggcactggc aacggtgtat ctggtgatta agttcaggca
     3841 ggagcgtgtc atcaaccgta gtctggtaga tgccaaggta caggaaatag tgatgtttga
     3901 gaaggcggaa tatgaaaaaa cttgcaaaaa ggatgcactg cgacaggcag cggtagtggt
     3961 tgattcgctg ttgcaggtgt ggggtgagca atcttcattc gacaccacag gcaggccacc
     4021 gttgccattc aaacccactg ctccggattt tcagtcgccc cttgatcggg taacactgaa
     4081 gcctttgttt gattcgatac agtcaattaa ataggctggt attacttcaa gagagatgct
     4141 aacaatgaac catgaaaggt agtcagggaa tgtttgagtt aaatgtttat agaacaaaaa
     4201 ggcagtaaaa atattttcgg acaaaattgt catttatagt aaacaatcat ttttttgcta
     4261 tatatatgcg ttcgaaaaat gagttcagcg gattttaaca tttaattttc tacatttatt
     4321 ttaaacaaaa tcatttaaaa attattcatt caatcaaata ttatcaggat actaatggag
     4381 aaagcaaaaa gaatacaatt tgaaagaaaa tcgtcaacgg atttttatca ggtactgagc
     4441 aagcgagttg aagattattt caagacgaac aacatttcaa aacatgccaa cggtgcaatg
     4501 gtatttaaga ccatttttgt gcttacagta tttattttga gttatattgg tatcatttcg
     4561 aatgtattcg ggcttggaat ggtattgttt ttatttgcag tgaatggttt ttttgctgcc
     4621 atgataggat tcaacatttc gcatgatgcg gtacatgacg cctattcgtc caacaaaaca
     4681 ctgaacagga tattgggtac cacattcaat tttttgggta cgagtgacta cgtatggaag
     4741 ataaagcaca acatcgtgca ccacacatac accaacatac cggatcatga ctcagacata
     4801 gaagatattc cgttcatcag acttactgaa gtgcaggagc gcaagtggaa ccatagattc
     4861 cagcatattt acatgttttt cttttatctg ttcactacat tgtcatgggt attcaagaat
     4921 gattatgtca attttttcag acgggactgg ggttggtaca aaaatccgga tcatccgaaa
     4981 gtagagtact tcaggttatt tttctacaag gcattgtatt acttcatgtt tttggtattg
     5041 ccttttctgg tggttgacca gccgtggtat tttatactgc ttggatttgt gattctgcac
     5101 tttgttgaag gtatgacact ggctttggta tttcagttag ctcatgtggt agaagaagca
     5161 caatttccgg agccgaatga tgaaggcaaa cttgaagaca catgggcagt acaccagcta
     5221 cgtactactg ccaacttcgc aagaaagagc tggttggcga attttatttg cggtggcttg
     5281 aattttcaga tagagcatca tttgtttccg gctatttgcc atattcatta cacaaagtta
     5341 gcacctattg taaagcagac ggcatctgaa tttggtcttc cttaccatga agcaaagaca
     5401 tttggtggtg cactcatgtc gcacatacgt attatgcaaa gattaggaac tgcttagaat
     5461 attcttaaaa aaggctttaa gataaagcaa aaaaagaggc aaatcaaaat gtatgcctct
     5521 ttttttgttt tgtaaaatgt tcactaacaa tggataatac aaaatttatt caaattgctc
     5581 aggatagaga atactgatag ttttcagaat ccgataacct tggtttatgg ctatttaatt
     5641 tggtattatt cctgcatatt tttgcaaagt aatacatatt aatcctatta aaagttaaat
     5701 gaacatatta tggaacagta caaaaatata tttggctcca tgagaaacaa gcagatgttg
     5761 atgcgtatca gtttcgggat tatctttttt tggtttgggg cattaaaatt ttttcccagt
     5821 gtgagcccgg cggaagaatt ggcatctgag accatttata ggttgacctt cgggatattt
     5881 tcaaaacaca caggcctgat attgctcgcc atccttgaat gtggactagg gctaatgttc
     5941 ctttcaggaa aatataccaa agcaaccatc ctgacagcca ttggtcacat gttatgtact
     6001 tttacaccat ttttcctgct accggaagca tcttttacac aggcacctta tgcattcaca
     6061 cttgtaggcc aatacatcat gaaaaatatc gtattcatag tcgcactaac tatgatgtgg
     6121 ccggaggctc agacgggagt catcagacaa caaaagaaca tcaaacgaat aatgcacgaa
     6181 gatgtagcag tataattttg aacaaacaac ctcaatatat taaagcaaag caaaccagca
     6241 gaaaccgaca gctaaaacct ttgtacgtgt cagtaacatg gcatgaagca aaggtttttt
     6301 ttattgtaat gctacatcag ggtgttgtac tcttaagcgg agaatgatgt accgcagcca
     6361 caagtagtct tggcattcgg gtttttgaag gtaaatccgc ggttgttgag gccttcgtcc
     6421 cagtcaattt ccataccgag cagataaatg ccgtgtaact tatccatgaa aactttcatc
     6481 ccgttgatga tgaattcttg gtccgaatcc tgtttatgat cgaacccgat catgtatgaa
     6541 aatccggagc agccaccgcc tttgacacca atgcggagcc cctggtcttc aggtatattc
     6601 tgctcacgcc taatgacctc caactgtttg atcgcacttt gggtgatggt aacaggtgta
     6661 gtagtagtct cgtttaattc gctcataatg tagaagctgt ttttgttgta aaatgataca
     6721 tacaaaacaa gaaatcaaga aattagttcg gaagctgata tgaaaactta aaagaaaaat
     6781 aacagtccgg aaccggggca ctgtaatata ttaaatatgc cacaccaacc ttagtttgga
     6841 cccaaaaaat caatgacaag acttttacgc ccgaatatca tcccgaagtg cccacctgat
     6901 aaaaaacggt ttgtacagaa aaggcaccac actaatttta ttaaaaaatt atatccatcg
     6961 gtagagatac gaccgaatgg gactttgatt gaaccattat aaaatatcct gaataatcag
     7021 aaaaattata aaattcttat atccataaaa tgattaagct atgaaaaatt taattctact
     7081 catttccatc ttgttgttta gttccgggcc ttaccttcat gctcagaaca gtttcagtac
     7141 cgactttgag tcatacaatg aaggagactg ggtggcaggc aacgacccta cccactggcg
     7201 tacatggtct tcagaaacag gtggcacatc agatgatgcg aaaataacat ccgaaagggc
     7261 ggcaagcggc accaagagct tcaagataca aaataccgtc accggtggcg ggccagagga
     7321 tctcatcctg aaactgggag aggcctacac taccggcact gttactttgg gctttaaaat
     7381 gtttatcgaa ccaggagggc gcggatactt taaccttcaa agcggagaac aacccggact
     7441 ttttggtctt gattttttct ttcaggaatt tggtgaggtt gtagggtggt cgcatcagaa
     7501 ttatatctat aacaccagta gtcatccgat aggagaatgg tttgactgta agatggtggt
     7561 tgatcctgcc aacaatatgt ggacattgtc aataaataat cagtgtattc aggtatatcg
     7621 aggcaattat gcttcagttt cgggtgtaga tttctttgct aatccgggca ccaattattt
     7681 tattgacgat gtttactaca actacgatcc tacgcctgta gtatatagcg gtgctgaagc
     7741 cggacttatc aatttgaaca ttattagttc gacccagata aaaggttttc ctttcagttt
     7801 ttctaacc
//
LOCUS       DAOQQY010000113         7711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3766_length_7711_cov_6.109065, whole genome shotgun sequence.
ACCESSION   DAOQQY010000113 DAOQQY010000000
VERSION     DAOQQY010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3766_length_7711_cov_6.109065"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLE23_06760"
     CDS             complement(<1..651)
                     /locus_tag="PLE23_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HMX82999.1"
                     /translation="MLDQNEISLIIMAAGHGSRFGGPKQLMPVGTNGEFLMDYSIEYA
                     IEQGIEEIVIVTNSSFETTLCNHLKKFDSRAQVTLKYQDIFTKEYDNTAVLYGTAIAM
                     LAGAVGCSNSVIVLNADDYYGREGFESVTETLLNNTNVEACLAGYHLETTLSDFGTVS
                     RAVCTSDVNNFLHDISEYHHIQKDLAGNISTSDGVLFSGKEPVSMNFWGFKQIFIDF"
     gene            complement(843..3320)
                     /locus_tag="PLE23_06765"
     CDS             complement(843..3320)
                     /locus_tag="PLE23_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMX83000.1"
                     /translation="MNVSVTKMPRYLSTILVIVLTSIGLWAQQNKPYETNNVISGTTF
                     YKNYYSAGVSTITMAPQNASSYGLSTTVGNITFHYTPKANFNGKDYVLIEYFESFPGS
                     PSFIGLELIVVNSVVISRIDYITTPKNQSVTIPVLDNDETTSFGLSLSSDIPYVLNGT
                     ATKSGNSLNFVPANNFTGVANINYLACDALNTCKMGTVVIKVLPDVAVVNDTLRYTIG
                     KNTNANIFLGLNGYDGISANPGHGTISTVSTDVVKYTPSNNYTGSDYFSIYKVVGGVT
                     YTKHIFTTIFNTTPSNKYAIDDYKATPKNTPVTFNLQANDIGSYVIIQPNTMTSPNGT
                     IQYLGSGNVKFTPNANFTGTASFTYKIGFPGYNTVIETGRVQVEVSDQIPVSKTYNLS
                     IPEEKPLVLKYFVPFTDWSFTKEANPKNGSLVIYPGQQTITLQGQTISGFNMAVYTPN
                     AGFVNNTDEFSLKYCTGTGVCSIVKFEVYVKLDPNSANTNYCLDNCVWAGDTDGDGVV
                     SVKDVLPIAMCMGVSGIERSNATTDWEAQFGNDWSNPYESSPVDLKHIDTDGDGFITS
                     ADTAAIQQSYGNIHQLPNTSLTKLGDKNIYFEPRDTFLTPGDDAIIDIHLGVQNKLMY
                     DAHGFTFKFDYINHPLITPDKIHMLFSSDNWFVRDASALSMVKFPTNGSMHVAYGRTG
                     SNPMTGFGTVGLVIVEDLEGIKSDDQGFMSFKFTEISYLDKNGEYVSIPDQIVTFKLG
                     KKNTPVIANDDNVIVFPNPAMNYVNVASTEGVSINAIRMFTPMGQLVKHVEGSTQLDL
                     SSLPAGLYVLQIETNGGVVTKKFQKVN"
     gene            3629..5047
                     /locus_tag="PLE23_06770"
     CDS             3629..5047
                     /locus_tag="PLE23_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83001.1"
                     /translation="MQNNKLFNILTYLDKSEIVRCRKYLHSPYFNNSENLANLFELFL
                     ELVVKDAEEIPKEKIWKKIFPKEKYDDVRFRKLCSDLLKLIEGFLAQQLYEENPIRQA
                     THLIEAVARKKMLKLYNTAMKTARLLSERQLYQPANHYFYEYQIERNYYDILSHQEKR
                     VSKTNVENIVKNLDIFYLAEKLKYYCSVLSRQYQVSHEYKLLFIDEIIAHLSKHDYTH
                     IPSIEVYYQILLTQTESEHQVHYFKLKEYLQKYSHLFPLGEAYEIYTYALNYCIRMTN
                     AGVKDFLNEYFIVYKELLQKNIINFSDEEHGPPHFKNIILASLRLGEYDWTEKFINDY
                     KDQLPADQRDNAVTFNLAQLYFYKNAYNKVIRYLHQVEYEDLTYNLNSKVILMCTYYE
                     LDEIEPLYSLMESFRVYLQRNNKIAANRRQNYINFIKFIKKLTKLIAGDKKRIAQFLE
                     ELAGTTGVVSSDWLRRKADELM"
     gene            complement(5056..>7711)
                     /locus_tag="PLE23_06775"
     CDS             complement(5056..>7711)
                     /locus_tag="PLE23_06775"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HMX83002.1"
                     /translation="SLENEKNILKERINFISLNAGNLSESLANTEFRLDKLAREKADF
                     QNQIDIATQDEQLKKQMLDEALQYLESIRSEHSLMKSDLDKSMQAIQELNKNIFELEK
                     KSAILDNQRESLQTDIARNEAALREREAEMKSSIDRLDTVKKSAENKEKLIHELINNQ
                     ELIKAKEENLKTELEENSKILSTLNRILDAKRNEYKLTLSIIENLEGFPESIKFLASN
                     KDWAKNVPLLSDTIYCQEEYRIALENFLEPYLNYYVVENVAEALKAVEMLRKAQKGKA
                     NFILLDNIPKVKKAVPPHDDFISAIECVEVEDKYKNMFAYLLDKVFIVGEEKSYEDLK
                     DTDVTLLSSNGAFIRRKFALSGGSVGLFEGKKIGRKKNLEILEKDITKKEKEEQALIH
                     KIQNLREELEKLKNEDLQARINVEKDELNKINQDKITLESKLENFNSYKKETSEKILF
                     AGEKLTQLHEEYKNNLELLNSKSLDSTALKEHFDKQDSTFKEAATKMSDASGKFNEQN
                     IEYIKQQNKLSTLVRELSYRDAQTLELSNQKQQLTNQQSTELKEIETAHKQLASIDKN
                     LQTWYADKSTRETSLTTAETEYFKTRGILNELEEQLRAANRQRQDLQLLINNLKDKFN
                     DVKFQLSVVGERLQIEFKTGINEIINKEPDPNDDVEALEAEVIKLKSRIETFGEVNPL
                     AVEAFNEMSERYQTIKQQEQDIIAAKESLLDTITEIENTATKQYLESFDQIRTNFIEV
                     FRSLFTADDTCDLILANPTNPLESEINIIAKPKGKRPQTINQLSGGEKTLTAIALLFA
                     LYLLKPAPFCIFDEVDAPLDDSNIEKFNKIIKKFSTDSQFIIVTHNKATMAAVDVIYG
                     VFMPEQGVSAVSAVDFRSLDHNLVLETN"
BASE COUNT         2306 a         1430 c         1400 g         2575 t
ORIGIN      
        1 aaagtcaata aagatttgtt taaatcccca gaaattcatc gaaaccggct ctttacctga
       61 aaacaacacg ccatcagatg ttgaaatatt ccctgctaaa tctttttgga tgtggtgata
      121 ttccgaaata tcatgcaaga agttgttaac atcagatgta catactgccc tacttacagt
      181 tccgaaatca ctcaaagtag tttcgagatg ataaccggcc aagcaagctt caacattggt
      241 attatttagt aaagtttcag tgacactttc aaaaccttct ctaccataat aatcgtcagc
      301 attcaaaaca attacggaat ttgagcaccc tactgcacct gccaacatag caatagctgt
      361 gccgtataat acagctgtat tgtcatactc ctttgtaaag atatcctgat atttcaatgt
      421 cacctgtgct ctactgtcaa acttcttcag atgattgcac aaagttgttt cgaacgaaga
      481 atttgtaacg atgacaattt cttctatacc ttgttcaata gcgtactcga tcgaataatc
      541 catgaggaat tcgccatttg tacctacagg catcaattgt tttgggcctc cgaaccgcga
      601 accatgaccg gctgccataa ttatcaagct aatttcattt tggtctaaca ttgtaattcg
      661 gcacgtattt cagaaaatat ttttatatac aaatcattgt tcttcagtca tagtttcaaa
      721 gataatacaa cttaaatgaa ccaataatac aattgcctaa ataaaaaaaa agcgccacat
      781 caagtagcgc ttccaggatt ataattcagt ctaacaaaac aaaaaaagta tttagaacta
      841 agttaattta ctttttgaaa ctttttggtt acaacacctc cgttggtttc tatctgtaaa
      901 acatacaggc cagcaggaag tgatgacaaa tcaagttgtg tggagccttc aacgtgtttt
      961 actaattggc ccatcggggt aaacattctt attgcattaa tacttacacc ttcagttgaa
     1021 gcaacattca catagttcat agccgggtta gggaatacta taacgttatc atcatttgct
     1081 attacaggtg tatttttctt accaagtttg aatgttacta tttgatcagg aatacttaca
     1141 tactcaccgt tcttatcaag gtaacttatt tcagtgaatt tgaagctcat aaaaccttga
     1201 tcatccgact taataccttc gaggtcttcc acgattacca agcctaccgt accgaagcct
     1261 gtcattggat ttgaacctgt acgaccataa gcaacgtgca tactaccatt tgtagggaac
     1321 ttgaccatac ttaaagcaga ggcatcacgc acaaaccagt tatccgaaga aaacaacatg
     1381 tgaatcttat caggagttat taaaggatga ttgatataat caaacttaaa agtaaatcca
     1441 tgtgcatcat acatcagttt attctgtact ccaagatgaa tgtcaattat tgcatcatca
     1501 cctggagtca gaaacgtatc acgaggttcg aagtaaatgt tcttatctcc gagtttggta
     1561 agcgatgtat ttggtaattg gtgtatgttg ccataagatt gctgaatggc agcagtatcg
     1621 gcagaagtaa taaacccgtc tccgtctgtg tctatatgtt taaggtcaac cggtgaactt
     1681 tcatacggat tagaccaatc atttccgaac tgtgcttccc aatcagtggt tgcattgctt
     1741 ctttcaattc ctgatacacc catacacatg gctattggta atacgtcttt aacactcact
     1801 acaccatcgc cgtctgtatc gccggcccac acacagttat caagacaata attggtattg
     1861 gcactatttg ggtctaactt tacgtaaact tcaaacttaa caattgagca cactcctgta
     1921 ccagtacaat atttcaaact gaactcatcg gtattattta caaacccggc attcggagta
     1981 taaactgcca tattaaaacc ggatattgtc tggccttgaa gggtaatagt ttgctgtccg
     2041 ggatagataa ctaatgaacc attcttcggg tttgcttctt tagtaaatga ccaatcagta
     2101 aacggaacaa aatatttcaa taccagaggt ttttcttccg gaatagaaag attgtaagtt
     2161 tttgatacag gtatttggtc ggatacctca acctgaacac ggcctgtctc tatcaccgta
     2221 ttatagcctg ggaaaccgat tttgtaggta aagctggcag taccggtaaa gttagcatta
     2281 ggtgtgaatt ttacatttcc tgaacccaga tattgtatag taccattagg gctcgtcatt
     2341 gtattgggct gaataattac atagctacca atgtcatttg cctgaagatt gaatgttaca
     2401 ggtgtatttt ttggtgtagc tttataatca tcaatggcat atttgtttga tggtgttgta
     2461 ttaaaaatag tagtaaaaat gtgttttgtg taagtaacac cgcctaccac tttataaata
     2521 ctgaaataat cagaaccggt ataattattt gatggagtat attttacaac atcagtagaa
     2581 actgtagaaa ttgtaccatg accaggattg gcagaaatac catcatatcc attgaggccg
     2641 aggaaaatat tcgcatttgt attctttcca atagtgtaac gtaatgtatc gttaacaact
     2701 gccacatcag gtagtacttt aataacaacg gtacccatct tgcaggtatt gagcgcatcg
     2761 catgccaggt aattgatatt ggctacacct gtaaaattat tggcaggcac aaaattgagc
     2821 gaattgccgg atttggtagc agtaccgttc agtacataag gaatatctga agatagtgat
     2881 aacccaaatg aagttgtttc gtcattatct aacaccggta tagttaccga ctggttttta
     2941 ggtgtagtga tataatcaat tcttgaaata acgaccgaat tgacaactat aagttcgagg
     3001 ccaataaaac ttggtgagcc agggaacgac tcaaagtatt caataagcac ataatcttta
     3061 ccattgaaat ttgctttcgg agtataatgg aatgtaatat taccaacagt agtactgaga
     3121 ccgtaagaag aagcattctg tggcgccatt gtaattgtag aaacacctgc cgagtaataa
     3181 tttttgtaaa aagtggtacc actaatcaca ttattagtct cgtagggctt gttttgctgg
     3241 gcccataaac caatacttgt aaggacaatc accaggattg tacttaggta gcggggcatc
     3301 tttgtaactg atacattcat gtctagtaag attttttgtc tttggaattt atttcatgat
     3361 acaaatttgt gggtatcaga attccttctc aaggacagat ttacttcaat gtgattattt
     3421 ttttcaaata tatgtttttt atatgtaatg tataaaaacc attatattgg gcaaatatac
     3481 aaatttttca actaacctgc aataaaaaaa tgtgaacttt ttacactttt tttcttcagt
     3541 tttacgtaag aagtatttaa aaggttttca ggtaagaaaa gaattgtaca tttgttggct
     3601 gtaaagatac aaatttatca gcataattat gcaaaataat aaattattta acattttaac
     3661 ttatctcgac aagtctgaaa tagtcagatg cagaaaatat ttgcattctc cttatttcaa
     3721 caatagcgaa aatctggcta atttattcga actcttcctc gaacttgttg tcaaagatgc
     3781 agaggaaatt ccgaaagaaa aaatctggaa aaagatattt ccaaaagaaa aatatgatga
     3841 tgtcagattt cgaaaactgt gttcggattt gctcaaactg atagagggct tcttggctca
     3901 acaattgtat gaagaaaacc cgattcggca ggcaactcac ttaatagaag ctgtagcacg
     3961 gaagaaaatg ctgaaactct acaatactgc tatgaagaca gcaagacttt tgtcagaaag
     4021 gcaactttat caacctgcca accattactt ttatgagtat cagatagaaa gaaactatta
     4081 tgacattcta tcgcatcagg aaaaaagggt ttcgaaaaca aacgtggaga atatcgttaa
     4141 aaaccttgat atattttatc tcgccgaaaa actgaaatac tattgttctg tgctaagcag
     4201 gcaatatcag gtttcgcatg aatataaatt gttatttatt gatgaaataa tagcacacct
     4261 atcaaaacac gattatacac acattccgtc tatagaagtc tattaccaga ttttgcttac
     4321 ccaaaccgag agtgaacacc aggtacatta tttcaagcta aaggagtatc ttcagaaata
     4381 ttctcacctt ttcccattgg gtgaagctta tgaaatatat acttatgcac tcaattactg
     4441 cattaggatg acaaatgccg gcgtaaagga cttcctcaac gaatatttta ttgtatataa
     4501 agaattgctt cagaagaata ttataaattt ttctgatgaa gaacacggcc caccgcattt
     4561 caaaaacatc atccttgcat ctttgcgttt aggtgaatat gactggactg aaaaattcat
     4621 caatgactac aaagatcaac tacctgctga ccagcgagac aatgctgtga ctttcaatct
     4681 ggcacagcta tatttttata aaaacgcgta caataaagta atcagatatc ttcaccaggt
     4741 tgaatacgaa gaccttacgt ataacttaaa ctcgaaagtc atcttgatgt gcacatacta
     4801 tgaactggat gaaatagaac ctttgtattc attaatggag agtttcaggg tttaccttca
     4861 acgaaacaat aagatagctg ccaatcgaag acagaactat ataaatttta tcaaattcat
     4921 taagaaacta accaaattga tagcaggtga taaaaagcga attgctcaat tcctggaaga
     4981 attggctggt actactggtg ttgttagctc tgactggctt aggagaaagg cagatgaatt
     5041 gatgtaaaat atcgcttagt ttgtctcgag tacaagattg tgatcgaggc ttctgaagtc
     5101 aacagcactc actgccgaca caccttgctc cggcataaat acgccatata tgacatcgac
     5161 tgctgccatt gtagctttat tgtgcgtaac aattatgaac tgcgaatcgg tagaaaattt
     5221 cttaattatc ttgttaaatt tctcgatatt tgaatcatcg agtggtgcat ctacttcatc
     5281 aaaaatacaa aaaggtgccg gcttcaaaag ataaagtgca aataataacg caatggctgt
     5341 gagggttttc tcgccacccg acaactgatt gatagtttga gggcgtttac ctttaggctt
     5401 agcaatgatg ttgatttctg actcgagtgg attagtcgga ttggcaagaa tcaggtcgca
     5461 agtatcatca gcagtaaaca gacttcggaa cacttcaata aagttggttc tgatctggtc
     5521 gaatgattct agatattgtt ttgttgcagt attttcaatt tcggtgatcg tatcgagcaa
     5581 tgattctttg gcagctatga tatcttgctc ttgttgtttg atcgtctgat aacgttcgct
     5641 catttcatta aaggcttcca ccgcaagtgg attcacttct ccgaaagttt cgattcttga
     5701 cttaagtttg atcacttctg cctctaaggc ctctacatca tcgtttgggt ctggttcttt
     5761 gttaatgatt tcattgatgc cggttttgaa ttcgatctgg agcctctctc ctaccacact
     5821 cagttgaaat ttaacatcat taaacttgtc tttcagatta ttgataagta gctgcagatc
     5881 ctgacgttgg cggttagcgg cgcgcaattg ttcttctagt tcattgagta tgccacgtgt
     5941 tttaaaatat tcagtttctg ctgtggtcaa tgaagtttct cgggtagatt tatcagcgta
     6001 ccatgtttgt agatttttat ctattgaggc gagttgttta tgtgcagttt ctatttcttt
     6061 aagctctgtc gattgctggt tggtgagttg ttgcttctgg ttactgagtt cgagggtttg
     6121 tgcatcgcgg tagctcaatt cccgaacgag tgtactgagt ttattctgtt gtttaatgta
     6181 ttcgatgttt tgttcgttga attttccgga ggcatcgctc atcttagttg ctgcttcctt
     6241 gaatgttgaa tcttgtttgt caaaatgttc cttgagtgcg gtgctatcta aagatttaga
     6301 attaagtagt tcaagattat ttttatattc ttcatgtaat tgtgtcaatt tttctcctgc
     6361 aaacaagatt ttctcggatg tttctttctt gtagctgtta aaattttcaa gtttgctttc
     6421 aagtgttatt ttgtcctgat taattttatt caattcgtcc ttttctacat tgattctcgc
     6481 ctggaggtct tcatttttta atttttcaag ttcttcgcgc agattttgaa tcttgtgtat
     6541 aagtgcttgt tcttcctttt cctttttggt aatgtccttt tcaagaattt cgagattttt
     6601 ctttcgtcca atttttttcc cttcaaacaa gcctactgaa cctccgctga gggcaaactt
     6661 acgtctaata aaagcaccgt tggatgacaa taaggtaaca tctgtatctt taagatcttc
     6721 ataagatttc tcttcgccta caataaatac cttatccaac aggtaagcga acatattctt
     6781 gtacttgtct tccacttcca cacattcgat agctgaaata aaatcgtcgt gtggtggcac
     6841 cgcctttttg accttgggaa tattatccag caatataaaa ttagcttttc ctttctgggc
     6901 tttacgcaac atttctactg ccttcaaggc ttctgctacg ttttcaacta cataataatt
     6961 gaggtaaggc tcaagaaaat tttcaagtgc aatacgatat tcttcctgac aataaatagt
     7021 atctgaaagt aatggaacat tctttgccca atctttattg gacgccaaaa acttaattga
     7081 ttccggaaat ccttcaagat tttcaatgat gcttagtgtc agtttatatt cattcctttt
     7141 tgcatcgagg attctgttaa gtgttgaaag aatcttgctg ttttcttcaa gttctgtctt
     7201 caggttttct tccttggcct taattagctc ttgattgttt atcagttcat gtatcagttt
     7261 ttccttattt tctgctgatt tcttaacagt atcaagtcgg tcaatagagc ttttcatctc
     7321 agcttcacgt tctctgagtg ctgcctcatt gcgtgctata tcggtctgca atgactcacg
     7381 ttggttgtca agtatggctg attttttttc gagttcgaaa atgtttttat tgagctcttg
     7441 tatggcctgc atacttttgt cgaggtcaga tttcatcaga gaatgctctg aacgtattga
     7501 ttcaaggtat tgcaaagctt catcgagcat ttgctttttt agttgttcgt cttgggtagc
     7561 aatatcaatc tggttttgaa aatcagcttt ttcccttgct agtttatcca gacgaaattc
     7621 ggtgttggcg agtgattctg acagattacc tgcattcagc gaaatgaaat tgatccgttc
     7681 tttcaggata tttttttcat tttcaaggga g
//
LOCUS       DAOQQY010000114         7669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3792_length_7669_cov_5.485028, whole genome shotgun sequence.
ACCESSION   DAOQQY010000114 DAOQQY010000000
VERSION     DAOQQY010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3792_length_7669_cov_5.485028"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>7669)
                     /locus_tag="PLE23_06780"
     CDS             complement(<1..>7669)
                     /locus_tag="PLE23_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMX83003.1"
                     /translation="YDIDLTNAPDVTRSTMATGSPNGGHPNTTDNTFGYEVLSGNNFT
                     EYLMNNGVLTQTVVYTFRPQIDFMSGAETEICYGPTFEIRVQVLPKVFNSATPDGSNL
                     VCQSATRLVEGVPGSFPAHPGPWTHEWVVASPQPAGFTGTGEFVDVNALPGGQSTTSQ
                     TGEFKGINTGMIRLLYYATPANGCAPFQPFVLDLEVIPQPALNPIGGPNMVCPGANAT
                     YAVSVDNSPANTYSWTLSSGGTFSTPTNGPSVGINWSTVLGGPHTLTATETSPNGCET
                     VNTYIINIVDMSDPTTMCPADITVGTSTDGDRGDCEYEVIGTFLDVIASDNCGIMSAT
                     HDYDFGGTSLHHKIFDLGTTTVTWTVKDNSGSTNTCTFNITVVDDEFPSVLCPHDVSV
                     NTDGTGLDIFSQLAGVTTNTSPDCGIILSNLAPTTTDNCNTIDVTWTATGSTPDNGSG
                     ALSNVEFNVGNTTVTFSADDTHGNVSTCSFNVQVVDNEKPAFTCPSNTILYTSDDAIA
                     NNCAYLVGDNTLDASAANDNCGVLSVVNNYNGTATLNGSTLPFGPTTIVWTITDVNGN
                     QNSCTYTLDVKDNEGPIVATCPSDITVTTNGNECDGFVSYELPRFNDNCGGVNQLGTL
                     ASGLAPDTDFPKGVTTVVYNYFDPSGNGPATCSFKVTVIDNQKPSISCPPNIIVNNDG
                     SGLNYQAFTYGITVDDTGNDCSVGFLGLNPVMSDNCPGPYILSHEITYPDGTTVASPG
                     SVNAIMFPVGVSTVAYIVRDQSNNTSSCTFTITVKDVEKPVFAQCASDVTISTSVNGT
                     GDCAITIPDLTAGVALIDNCSSILNITVTQSPAAGTIFAGSHGSVQVVTLTATDQSGN
                     TATCTAKVTIRDDENPVAQCATPYELYLDQSGNASIAVADINNGSFDNCHINNLAINT
                     TQVSCAEAGNTVMVTLTVTDNAGNLATCTTPVMVLDTLAPVIKNCPSNIVQGNDPDMC
                     MAFVNFSKVQSTDNCDTDVDIYYWTSGATVLGGPDIFGQSGDADPSNDPILGQANAKN
                     YNVGITTVHYFAVDNFGNISEECTFTIDVRDAQKPVITKCPDNRDIFVNAGTCSGTVP
                     NMIPELIYKDNCAVTVTQSPVAGTLFGNAHNDTQVVTFTVTDAGGNTATCTTVLTLKD
                     NIAPIITTCPPSRNVGTVLNTCSGVVPNMIPELIYTENCPNVTVTQTPVAGTSFGAAN
                     NDTQVVTFTVTDAAGNTATCTAVLTLKDTQAPTIICPPNVNVPCTISKITIPKPVTSD
                     NCGVASVTNSKNGTDDASDIYAPGTTTVVVWTVTDVNGNQATCSMTVTVAPVPTLTVT
                     GAGSYCKDGFIPDAVATSSTTGTFSWYKNAALTIPVNPAQVSGPNNNTYHPTGILGTE
                     TVYVVITDPVSGCVSNPVSVTYNIVACSVTSTDPCHCKNNATTLTNGQFDEEITIFAP
                     SGQTWHIVTATGLYKTSSPAPPAAPIPVTTGVGSPDKPIAVAPGVYRMNGIHVDGLGY
                     NITFANGLGDTLTLSNLCYYPNPQFVGLNPDYCANHPVVQLQGTAAPSVALNERFDLY
                     DATNTIAVQLNITQLNPALLAPGTYTVRYTFTAQPNTPNTQYAGCEQTITMQVKIFPV
                     PPVELACGSLHNVSLDPSGIATITSDVVLEGNYGCWSQYQVSIEGKTNNIVTCADLGK
                     TFKVSIIDPFTGNKCWGNILVEDKLAPTITCADVTVSCNNDGLDPSLVGYPVFKDNCG
                     VDPATITYNETINNPTCTGDFSAVITRTWFARDYYGNSATPCTQTISVKRGTLAEVVF
                     PPNLDDKDAPSISCVNPNTNPAVTGVPTIDGKPINKFCELMVAPPQDVVIPICDNSYK
                     IVRTWTVMDACSNNMITGIQVIIVKDKEGPSLTCPKPSEFNYTVSGNGNKCLAIIQAP
                     KINVQDNCSQAKNVNVKTKVTVNGIVTELNSNGGIFQIPFGIYTFTYTATDACGNITS
                     CSVDVDIKENEPPVAVCQELTVVSLINPVTLVNATSFDSGSYDECGGALKYTVRRMDN
                     PACPGTDATTFADKVPFYCCDVNNGPVTVVMRVTDASGNYNECMVQVTVVDKIKPAVL
                     CPSDISVQCGQPYTPEDPAVISFDKKVDKTITINFPVTLEEKIVVSGLPPYATIEDIN
                     LGLDIKHDYIDQLTLKLISPAGTVVTLFQGGSCGAFTTDINATFDDEGAPFFCNGNPT
                     AIVGNVQSQYNALSYFDGENPNGIWTVQVIDGAPLGGGTFNSAKLTISYNTPLSLKPI
                     VTDNASDCGVNLTWVDANSPGQCQNNVTITRRWIATDKSGNTGFCDQKVKLIDTTPLD
                     ILFPADVTITECKKFEDLAGLKSISHNGDCELVAVEYIDEIFDVVPDACYKIIRTWKV
                     LDWCKYNPSANNTEGGIVLNNNPRHLRDDGDGYFIVKQVIKVIDNEKPHFYSCPKDTV
                     ICSMAEDCNNNPVSITKLAYDGCAKPDQLKYEWKLDITANGTYDISGSTPTMNVSVPL
                     GKHRVWFKVTDGCGNYDICDFTFTVNDCKKPTPVCHKGLSTDIMVTGMVAIPAKAFNA
                     SSSDNCTAPGKLKFSYST"
BASE COUNT         2079 a         1582 c         1759 g         2249 t
ORIGIN      
        1 agttgagtaa gagaatttta gcttacccgg agctgtacag ttgtcagaac tacttgcatt
       61 gaacgcttta gcaggaatag ccaccatacc tgtaaccata atgtcggttg ataaaccttt
      121 atggcatacc ggtgtaggct tcttacaatc gttaacggta aatgtaaagt cgcaaatatc
      181 gtagttgcca caaccatctg taaccttaaa ccatacacgg tgcttgccaa gaggtacaga
      241 tacattcata gtaggtgtgc tacctgagat atcatacgta ccattagctg taatgtccag
      301 tttccattcg tatttgagtt ggtctggttt ggcacagccg tcgtaagcca gtttggtaat
      361 cgatacaggg ttgttgttac aatcttctgc cattgagcag ataacagtat ccttagggca
      421 agagtagaag tgaggttttt cattgtctat caccttaatg acttgtttta caatgaagta
      481 tccgtcacca tcgtctcgta agtgtcttgg attgttattg agaacaatac cgccttcggt
      541 gttgttggca gaagggttgt atttacacca gtcaagcact ttccatgtac ggatgatttt
      601 gtaacaagca tctggtacca catcgaatat ttcatcaatg tattctactg ctacgagttc
      661 gcagtcaccg ttatgagaaa ttgacttcag acctgcaaga tcttcgaatt tcttacactc
      721 ggtgatagta acatcagccg ggaaaaggat atcaagaggt gtagtgtcta ttagtttgac
      781 tttctgatca cagaaaccgg tattgcccga tttgtcagtg gctatccatc ttctggtgat
      841 ggttacattg ttttggcatt gccctggaga gtttgcatct acccatgtaa ggttgacgcc
      901 acaatcagaa gcattgtctg taacaatcgg tttgagtgac agaggtgtat tgtatgaaat
      961 ggtcagtttt gcagagttaa atgtaccacc gccaagtggt gctccatcta ttacctgtac
     1021 tgtccagatt ccgttcggat tttcaccgtc gaagtagctt agtgcattgt attgtgactg
     1081 tacgttgccc acgatggcag tagggtttcc gttgcagaag aacggagcgc cttcgtcatc
     1141 aaatgtagca ttgatgtcag ttgtaaatgc accacatgaa ccaccctgga atagtgttac
     1201 tactgtacct gcagggctta tcagtttgag tgtcaactgg tcgatgtaat cgtgtttgat
     1261 gtcaagaccg aggttgatat cttcaattgt agcataagga ggcaaaccac tcactactat
     1321 cttttcttcc agtgtcaccg ggaagttgat ggtaatggtc ttgtcaacct tcttgtcaaa
     1381 tgaaatgact gcagggtctt caggtgtgta tggctgacca cactgtactg aaatatcaga
     1441 aggacatagt acggcaggct tgattttgtc aacgacagtc acctgtacca tacattcatt
     1501 ataattgcct gaagcatcag ttactctcat caccacagtt accggcccat tgttgacatc
     1561 gcaacaatag aatggaacct tatctgcgaa tgttgtagca tcggtgccag ggcaagcagg
     1621 attatccatc cttcttacag tgtatttcaa tgcaccacca cactcatcat aactgccaga
     1681 gtcaaatgaa gtagcattta caagtgttac cggattaatc aaagatacta cagtaagttc
     1741 ctgacaaact gccacaggtg gttcgttttc tttgatgtca acatcgactg aacagctggt
     1801 aatattgcca caagcatctg tagcagtata agtaaatgtg tagattccga aaggtatctg
     1861 gaatattcca ccattgctgt taagctcagt gacgattccg ttcacagtaa ccttggtttt
     1921 aacgtttacg ttcttcgcct ggctacagtt gtcttgtaca ttgatcttcg gagcctgaat
     1981 aatggcaagg cacttattgc cattaccgct gactgtatag ttaaattcag aaggcttagg
     2041 gcaagtcagt gatggacctt ccttgtcttt gacgataatt acctgtatgc cggtaatcat
     2101 gttattcgaa caggcatcca ttactgtcca ggttcttact attttgtaac tgttatcaca
     2161 gattggtatc actacatctt gaggaggagc taccatcaat tcacagaact tgttgatagg
     2221 ttttccatcg atggttggta cacccgtaac agccgggttg gtgtttggat ttacacaact
     2281 aatagatggt gcgtctttgt cgtcaaggtt tggtgggaat accacttcgg caagtgtgcc
     2341 tcgttttaca gaaatagtct gtgtacaagg ggtggcgctg ttgccgtaat agtctctcgc
     2401 aaaccatgtt ctggtaatga cagcagagaa atcgcctgta catgtagggt tgttgatggt
     2461 ttcattatag gtaatagtag caggatcaac gccacaatta tccttgaata ccggataacc
     2521 aacaagtgaa gggtcaagac cgtcgttgtt acaacttaca gtaacatcag cacaagtgat
     2581 ggtaggtgcg agtttgtctt ctaccaggat gttgccccaa catttgttac cggtaaacgg
     2641 atcgataata cttaccttga aggttttgcc aagatcagca catgtaacaa tattatttgt
     2701 cttaccttca atgctcactt gatactgcga ccagcagccg tagtttcctt caagaactac
     2761 gtctgaagta atagttgcaa tacctgaagg atctagtgaa acattgtgaa gacttccgca
     2821 tgcaagctcg acaggaggca cagggaaaat cttcacctgc attgttattg tctgttcaca
     2881 tcctgcatac tgcgtgtttg gagtgttcgg ttgtgctgta aatgtgtatc ttacagtata
     2941 agtgcccgga gccaatagtg caggattaag ctgtgtaata ttgagctgta ctgcaatggt
     3001 gttggtagca tcatacaagt caaaacgttc gttaagtgcc acagatggag ctgctgttcc
     3061 ttgcaattgg acaaccggat ggttagcgca atagtcaggg ttcagtccaa caaactgtgg
     3121 atttgggtag taacaaaggt tactcagggt cagtgtatca ccaagtccat ttgcaaatgt
     3181 gatattgtat cccaggccat ccacatgaat accattcatt ctgtaaacgc caggagctac
     3241 agctatcggt ttatccgggc tgccaacacc ggtagttacc ggaattggtg ctgcaggagg
     3301 agcagggctg gatgttttat ataatccagt tgctgttaca atatgccaag tttgaccgga
     3361 tggtgcaaag atggtaattt cttcatcaaa ttggccattg gtcaaagttg tcgcgttgtt
     3421 tttgcagtga caagggtcag ttgaagtaac agaacatgca acgatgttgt aagttacaga
     3481 tacaggattg ctcacacaac cactaacagg atcggtaatg acaacataaa ctgtctcagt
     3541 accaagaatg ccggtagggt ggtatgtgtt attattagga cccgagacct gagccggatt
     3601 gaccggaatg gtaagggctg cattcttata ccaactgaat gtgcctgttg tagagcttgt
     3661 agctactgca tcaggaataa aaccgtcttt gcaatacgag ccggctccag taacagtcag
     3721 tgtaggtaca ggcgcaacag ttacagtcat cgagcatgtt gcctgatttc cattcacgtc
     3781 agttactgtc caaactacca cagtagtagt gcctggtgca taaatgtcgc ttgcatcatc
     3841 cgtgccattc ttgctgttgg taacagaagc cacaccacaa ttgtctgaag taacaggttt
     3901 tggtatggtt attttgctta ttgtacacgg tacatttaca ttaggagggc agataatggt
     3961 aggtgcctgt gtatccttaa gtgtaagtac agcagtacat gtggcagtat tacctgctgc
     4021 atctgttact gtaaatgtta ccacctgtgt atcattgttg gcagcaccaa aagaggtacc
     4081 tgcaaccggt gtttgggtaa ctgttacatt agggcaattt tcagtataaa taagctccgg
     4141 aatcatgtta ggcacaacac ccgaacaagt atttaatact gtacctacat tgcggctagg
     4201 cgggcaggta gtaatgatag gagcaatgtt gtccttcaat gtcaggacag tagtacatgt
     4261 tgcagtattt ccaccagcat cagtgacggt aaatgttaca acctgtgtat cattatgtgc
     4321 attaccaaat agcgtacctg caacaggtga ttgtgtcacc gttacggcgc agttgtcttt
     4381 gtaaataagt tccggaatca tgttcggtac tgtacctgag caagtaccgg cattcacaaa
     4441 gatgtctctg ttgtcagggc attttgtaat gactggcttc tgtgcatccc taacatcaat
     4501 ggtgaatgta cattcctcag aaatgttgcc aaagttatcc actgcaaagt agtgtacagt
     4561 tgtaatgcct acattgtagt ttttagcatt tgcctgaccc aaaataggat cattcgaagg
     4621 gtcggcatca cctgattgtc cgaaaatgtc aggaccacct aatactgtag cacccgaagt
     4681 ccagtaataa atatcaacat cggtgtcgca gttgtcagtt gactgtactt ttgagaagtt
     4741 gacaaatgcc atacacatat ccggatcatt gccttgcact atgtttgaag ggcaattttt
     4801 gatgactgga gcaagtgtgt caagtaccat aacaggtgta gtacaggtgg ccaggttgcc
     4861 ggcattgtcc gttactgtaa gtgtaaccat aacggtattg cctgcttcag cacaagatac
     4921 ctgcgtagtg ttgatagcaa gattattaat atgacaatta tcgaaagagc cattgttgat
     4981 atcagctacc gcaatagaag cattgccact ctggtcgaga tacaactcat aaggagtagc
     5041 gcactgagct accggatttt catcatcacg aatggtaact tttgcagtac aagtcgcagt
     5101 attgccggat tgatctgtag cagttagtgt tactacttgt acagagccat ggctacctgc
     5161 gaatatagtg cctgcggcag gactttgtgt tactgtaata tttagaattg agctacagtt
     5221 gtctattaat gcaacacctg ctgtcaaatc aggaattgta atagcacagt ctcctgttcc
     5281 atttacactt gtgctgatgg ttacatcaga tgcacattgt gcaaatactg gcttttctac
     5341 atcttttaca gtaatggtaa atgtacatga gctggtatta ttcgattgat ctctgactat
     5401 gtatgccact gtagataccc caacagggaa catgatggca ttcacacttc cgggagaagc
     5461 tactgttgtg ccatcaggat aagttatctc atgcgaaagt atataagggc cagggcaatt
     5521 gtcagacatt acaggattca aacctaagaa tccaacagaa caatcattgc ctgtgtcatc
     5581 tacagtaata ccataagtaa aggcttgata attcagtccc gaaccatcgt tgttgacaat
     5641 gatattaggc gggcatgaaa tactaggctt ttgattgtca attactgtaa ctttgaagct
     5701 gcatgtagct ggtccattgc ccgaagggtc aaaatagtta tacactactg tagtaacacc
     5761 tttcgggaaa tcagtgtcgg gagcaagccc tgaggcaagc gtaccaagct gattcactcc
     5821 gccacaatta tcattgaaac gaggcaattc gtatgaaaca aacccatcgc actcgttgcc
     5881 attggtagtg acagtaatat cagacggaca agtcgctact atagggcctt cgttgtcttt
     5941 gacatcaagc gtgtatgtac acgaattttg attgccattc acatcggtga tggtccagac
     6001 tatagtggta gggccaaatg gcaatgttga accgttgagt gtagcagtac cattgtaatt
     6061 gtttactaca gagagtacac cacagttgtc attggcagcg cttgcatcca gggtgttatc
     6121 acctaccagg tatgcacaat tatttgctat agcatcatct gaagtataaa gaatggtatt
     6181 tgacggacac gtgaaggccg gcttttcatt atcaactacc tgaacattga agctgcaggt
     6241 gctaacgttt ccgtgtgtgt catcagcaga gaatgttaca gtggtgtttc ccacattaaa
     6301 ttctacattt gataaagcac cacttccgtt atccggagtt gaacctgttg ctgtccaggt
     6361 aacatcaatg gtattgcagt tgtctgttgt agtcggagca aggttgctca ggatgattcc
     6421 acaatccggt gatgtattgg tagtgacacc tgccagttgt gagaaaatat ccagacctgt
     6481 accgtctgtg ttaaccgaaa catcatgagg gcataaaact gaagggaatt cgtcatcaac
     6541 tactgtaata ttaaacgtac aagtattggt agagccactg ttgtctttta ctgtccaagt
     6601 gacggtggta gtaccaaggt caaaaatttt gtgatgaagg cttgtgcctc caaaatcata
     6661 gtcgtgggta gcagacatga tgccgcagtt gtctgaagca ataacatcca ggaatgttcc
     6721 tatcacctca tattcgcagt cacctctgtc accatctgtt gatgtgccga ctgtgatatc
     6781 agcagggcac atagtggtag ggtcgctcat atccacgata ttgataatgt aagtattgac
     6841 agtctcgcaa ccatttggag atgtttctgt ggctgtcaat gtgtgtggac cacccaatac
     6901 tgtgctccag ttgataccaa ctgatggtcc atttgtaggt gtactgaatg ttcctcctga
     6961 agacaaagtc cagctgtacg tgttggcagg cgaattgtca acagaaacag cataagtagc
     7021 attagcacca gggcaaacca tattcggtcc acctataggg tttaatgccg gttgtgggat
     7081 tacctcaagg tccaggacaa aaggctggaa tggtgcacaa ccattagctg gtgttgcata
     7141 atacaatagt cggatcatac cggtattgat acctttgaac tcacctgtct gagatgtagt
     7201 tgactgtcca cccggaaggg cattaacatc tacaaattct ccggttcctg taaatccggc
     7261 cggttgaggg ctagctacaa cccattcgtg tgtccatggg ccaggatgag cagggaacga
     7321 acccggcaca ccttctacaa gtcttgtagc actttgacat acaagatttg atccgtcagg
     7381 tgttgctgag ttgaatacct taggtaatac ctgtactctg atttcaaatg ttggtccata
     7441 gcagatttct gtttcggcac cactcatgaa gtcgatttgt ggtctgaagg tatatactac
     7501 ggtttgtgta agaacaccat tgttcataag gtattctgta aagttgtttc cggatagaac
     7561 ttcatatccg aatgtgttat cggtggtatt gggatggcct ccgttcggtg acccggtagc
     7621 catcgtagat cgtgttacat caggcgcatt tgtcaggtca atatcatat
//
LOCUS       DAOQQY010000115         7668 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3795_length_7668_cov_7.302509, whole genome shotgun sequence.
ACCESSION   DAOQQY010000115 DAOQQY010000000
VERSION     DAOQQY010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7668)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3795_length_7668_cov_7.302509"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLE23_06785"
     CDS             <1..331
                     /locus_tag="PLE23_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HMX83004.1"
                     /translation="ETDAGGFTPRGISCEGEESTFIERFKKVHAWNDYFEPYDIRITK
                     GGSGADVGHLKPFGTFLMGLRPDSQRYFDIHHAVTDTIDKVNRRELALGSATMASIIY
                     LIDKYGL"
     gene            333..878
                     /gene="hpt"
                     /locus_tag="PLE23_06790"
     CDS             333..878
                     /gene="hpt"
                     /locus_tag="PLE23_06790"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HMX83005.1"
                     /translation="MSASETVRIDDLSFIQFIPAEQIAQRVAAMALDIDKKLLGKEPV
                     FLGILNGCFIFMADLVRHCNCEHEVKFIKLSSYEGTGSTGKVISEGKLSESLKGRHVL
                     LVEDIIDSGLTIKWITEKLQSLGVASITVATLLLKPDAIEYPVQIDYVGFEIPNNFVV
                     GYGLDYNGKGRNLKSIYTLVE"
     gene            975..1048
                     /locus_tag="PLE23_06795"
     tRNA            975..1048
                     /locus_tag="PLE23_06795"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1007..1009,aa:Gln,seq:ttg)
     gene            complement(1377..1886)
                     /locus_tag="PLE23_06800"
     CDS             complement(1377..1886)
                     /locus_tag="PLE23_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMX83006.1"
                     /translation="MGHKQSDKVRETLARAKKERKSTQKILKAMRGIRPEILDRNFMA
                     AHEKVFAGTDCLECANCCKTSSPIITNKDIDRMAAHLRMKPGDFVVKYLRIDEDEDYV
                     FQQTPCVFLNDDNTCSIYEHRPKACHEYPHTDRKNMREILELTYKNSLVCPAVYEIIR
                     EVESLIARK"
     gene            complement(2145..3035)
                     /gene="atpG"
                     /locus_tag="PLE23_06805"
     CDS             complement(2145..3035)
                     /gene="atpG"
                     /locus_tag="PLE23_06805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HMX83007.1"
                     /translation="MGANLKEVRDRIKSVISTQQITKAMKMVSAAKFRKAQQAITQIR
                     PYADRLNRMMKNIAANTDGGSTLVYNQERDVKKVMLVVVTSSRGLCGAFNTNVVKAAV
                     ASIEGKYSQVYKDGNLSILCIGKKGFDGLKRRYPKAHFNTESVDLFMNLAFENVASVS
                     GKIMGLFAGKEYDEVAVAYSRFKNAAMQFAEVEQFLPIPKQDETTQKKVKADYIFEPD
                     QDTLLETLIPNILQLQFHKYLLDTHASEHGARMTSMDKATENAEELLKALKINYNKAR
                     QESITKELLEIVAGAAALEG"
     gene            complement(3067..4659)
                     /gene="atpA"
                     /locus_tag="PLE23_06810"
     CDS             complement(3067..4659)
                     /gene="atpA"
                     /locus_tag="PLE23_06810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMX83008.1"
                     /translation="MVDVKPDEISAILKQQLSGMSTAQQLEEVGTVLQVGDGIARVYG
                     LNSVQAGELVEFENGVQAIVLNLEEDNVGVVLLGSSDGINEGNTVRRTRKIASLQVGE
                     GMLGRVVNPLGEPIDGKGPIAGKTYEMPLERKAPGVIFRQPVTEPLQTGIKAIDAMIP
                     IGRGQRELIIGDRQTGKSAIAIDTIINQKEFYEKGEPVYCIYVASGQKASTIAQVARI
                     LEENGALPYTVIVSASAADPAPLQFYAPFAGASIGEFFRDTGRPALIVYDDLSKQAVA
                     YREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKVINNDEIARNMNDLPESLKAAGIV
                     KGGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLESNLFNSGVRPAINVGISVSR
                     VGGNAQIKSMKKVSGTLKLDQAQYRELEAFAKFGSDLDAATKAVLDKGKRNVEVLKQP
                     QYSPLTVEKQVAIIYLGTNGLLRDVPVENVREFEDMFLTSLERQHPEVLAAFKKGDLP
                     ADGLKLVAQLAQDLSAQYKSRG"
     gene            complement(4686..5243)
                     /gene="atpH"
                     /locus_tag="PLE23_06815"
     CDS             complement(4686..5243)
                     /gene="atpH"
                     /locus_tag="PLE23_06815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HMX83009.1"
                     /translation="MSVQRVATRYAKSLIDLARDNNKLDRIKEDVMGFLEVAKNRDFK
                     MCLQSPIINTGTKTKIFRELFDGKVDQVTMAFFNIILNKGRESYLPEIASEFIEQYKA
                     INHISTIRLVTATEVDAKTIDAIRQKFEAAGSTRSNVEIIHTVDPALIGGFLVEFDDK
                     LYDASVKYQLEQVKKQFTGNLHIQN"
     gene            complement(5269..5787)
                     /gene="atpF"
                     /locus_tag="PLE23_06820"
     CDS             complement(5269..5787)
                     /gene="atpF"
                     /locus_tag="PLE23_06820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMX83010.1"
                     /translation="MNMILLADFSVIRPEPGMLVWTTLIFLLFWFILGKLAFKPIGAA
                     LKSRENSIQDALDEAKKAREEMEQLKAENDKILTEAREERSKMLKEAKETKDAIIAEA
                     RTKAKEDAQKIVSSAMNEIENQKKAALTEVKNMAGMMAIEIAEKVIKKELKGNTEQEA
                     YVNTLVKDIKLN"
     gene            complement(5884..6084)
                     /gene="atpE"
                     /locus_tag="PLE23_06825"
     CDS             complement(5884..6084)
                     /gene="atpE"
                     /locus_tag="PLE23_06825"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004569351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMX83011.1"
                     /translation="MYAAIGAGLAVIGAGLGIGSVGGKAMDAIARQPEAVGDIRSNMI
                     LMAALVEGAALICMVLAFFVGG"
     gene            complement(6108..7316)
                     /gene="atpB"
                     /locus_tag="PLE23_06830"
     CDS             complement(6108..7316)
                     /gene="atpB"
                     /locus_tag="PLE23_06830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMX83012.1"
                     /translation="MAQHEGQEHVNESPTATAAGHADAETGHDAHAEGEFKPGEFAVH
                     HIADANVFQLVGETYLPLPCILYAPDKGWTIMSSGAFVPKHHGNGENAVDGYILSGGN
                     VRRVADASFPTGTVAIQGIKVKEEDVNGKKKEVHYALYNGQEYKTDARSAFDGGIVGG
                     GITSFYDYSITKNVFTMILVFVLLLILFISVRNAYTRREGMAPKGLQSFMEPFITFVR
                     DDVAKPNIGHSYEKYMPFLLSLFFFILGLNLIGQIPFFPGSANVTGSISVTLVLAVLT
                     FLMTNLNGNKHYWGHVLWMPGVPAILKILILTPLEILSLFLKPFTLLLRLFANITAGH
                     IVILSFVGLIFVFGKSGASIGGTALGAAISVPLTLFMSTIELLVAFLQAYIFVMLTAS
                     YIGAAVEEHH"
BASE COUNT         2113 a         1749 c         1440 g         2366 t
ORIGIN      
        1 agaaacagac gcaggcggat tcacaccaag aggcatatca tgtgaaggcg aagaaagcac
       61 cttcatcgaa aggttcaaga aagtacacgc ctggaacgat tatttcgaac cctatgacat
      121 caggattaca aaaggcggat cgggtgctga tgtcggccac ctgaaaccat ttggtacatt
      181 ccttatgggc ctcagacccg actctcaacg atatttcgat atacaccatg ctgtgacaga
      241 taccattgat aaagtcaaca ggagagagct tgccttaggc tcggctacca tggcatccat
      301 catttatctc atcgacaaat acggattata atatgtcagc aagcgaaaca gttcggatag
      361 atgatctgag ttttatccaa ttcattcctg cagagcagat agcgcaaaga gtagccgcaa
      421 tggcacttga cattgacaaa aaactacttg gtaaagaacc tgtatttctg ggcatactca
      481 atggctgttt tatttttatg gccgacctgg taagacattg taactgcgaa cacgaggtaa
      541 aatttatcaa attaagttcg tatgaaggta ccggctctac aggtaaagta atatcagaag
      601 gtaagctgag cgaatcactc aaaggccgac atgtgttact tgttgaagat atcatagatt
      661 caggcctgac cataaagtgg ataaccgaaa aactccaatc actcggtgtt gcatccatta
      721 ctgttgctac cttgttactc aagcctgatg ccatagaata ccctgtacag atagattatg
      781 tcggatttga gattcctaac aattttgtag taggttatgg tcttgattat aacggcaagg
      841 gccgcaatct aaagagtatt tatacattgg tagaatagta aaatgaaatt cagaaagaaa
      901 tttgtgtaaa aaaatttgtt tccctcttta cacattcaaa aaaatcactt atctttgcat
      961 cgctttttgc aaattggtct atggtgtaac ggtagcacaa ctgattttgg ttcagttagt
     1021 tgaggttcga atccttgtag accaaccaag ccttacttat tatgagtgag gcttttttta
     1081 tttaattaaa atttttatta tttttttttg gtaaaataaa aataataaaa tacctttgag
     1141 tgtctttaca atgaaaggct cctcccaaga gcttaagata accgagaaaa ccaaatttac
     1201 ctttttacag ccgatttata ataacaactg aagccacaaa ggctttgtgt tattttttat
     1261 acttgaatat ttgtccgttc tattgaatgg agatagacat atctattatt tattttcaaa
     1321 caatagataa tcccgtggac gcaggagtca ggtcggcata gctggtctgg ctttttttat
     1381 ttcctggcta tcagagattc tacttctctg atgatttcat atacagccgg gcacaccaat
     1441 gaatttttgt aggtgagttc gaggatttca cgcatattct ttctgtccgt atgcggatat
     1501 tcatggcaag ccttcgggcg atgttcgtat atgctgcaag tattatcgtc attcaggaac
     1561 acacaaggtg tctgttgaaa aacatagtct tcatcttcat caatacgcag atatttcact
     1621 acaaaatcgc cgggtttcat acgcaggtga gcagccattc ggtctatatc cttattggtt
     1681 attatcggac tggaggtctt gcagcaattg gcacattcca gacaatccgt cccggcaaat
     1741 actttttcat gggcagccat aaaatttcga tccagaattt ccggacggat tccacgcatt
     1801 gccttcaaaa tcttttgtgt tgatttgcgc tccttctttg cccgggcaag cgtttctctt
     1861 actttgtcgg attgtttgtg acccatcagc ctgatatata attcaaaata aaatattgat
     1921 aaatcaaatt aattcttcct ataattatta gaaacacttc attttacttt aaaacaaata
     1981 aataaatcaa gctgttggtg aatttatttg ctcagggctg ctgtacaccc atgacagaag
     2041 gtttatgcag tgtttccgca ttcccgagtg agcggggtgt cggatagttt ataaaaaaat
     2101 agccttacat tactgcaagg ctattttttt atagaatccg tttatcaacc ttcgagtgca
     2161 gcagctcccg ctactatctc taataattct ttcgtaatac tttcctgtct cgccttgttg
     2221 tagttgattt ttaacgcttt caacagctct tctgcgtttt ctgttgcttt atccatcgaa
     2281 gtcatcctgg caccatgctc actggcatgc gtatcgagga ggtacttgtg gaactgtagc
     2341 tgaagtatat taggaatgag tgtttcgagt aatgtgtcct ggtcgggttc gaaaatataa
     2401 tcggccttta ccttcttctg tgtggtttcg tcctgcttag gtatcggcaa gaactgctct
     2461 acttctgcaa actgcatggc tgcattctta aaccgactgt aggcaactgc tacctcgtcg
     2521 tattcttttc cggcgaacaa acccattatc ttaccagata cacttgcaac attctcaaac
     2581 gcaaggttca taaacaaatc gacactctca gtattgaaat gtgccttcgg atatcgcctc
     2641 ttcaacccgt caaatccttt cttaccgata caaagaatag agagattacc gtctttatat
     2701 acttgcgaat atttcccttc gatactggca acggcggctt taacgacgtt ggtattgaaa
     2761 gcaccacaaa gtcctcttga agaagtgact accaccagca tcactttttt gacatcccgc
     2821 tcctgattat atacaagtgt agaacctcca tcagtgttgg ctgcgatgtt tttcatcata
     2881 cggttaagtc tgtcggcata agggcgtatc tgggtaatag cctgctgtgc ttttctaaac
     2941 ttcgcagcag acaccatctt catcgccttg gtaatctgtt gtgtagagat taccgacttg
     3001 atcctgtctc ttacttcctt taagtttgct cccataattt atgagctttc gatgctttta
     3061 attgaattat cctcttgatt tatattgcgc actcaaatcc tgagccaact gtgctaccaa
     3121 cttcagacca tcagcaggca aatctccttt tttaaatgct gcgagcactt caggatgttg
     3181 tctttcaaga gaagtcagga acatgtcttc aaattctctt acattctcta ccggcacatc
     3241 tctcaaaagt ccattcgtac caagataaat gatagctacc tgcttctcta ctgttagtgg
     3301 agaatactgt ggttgcttca atacctctac gtttctcttt cctttatcaa gaactgcctt
     3361 ggtagcagca tcgaggtcag aaccgaattt ggcaaatgcc tcaagctcgc ggtactgagc
     3421 ctgatcgagc ttaagtgtac ccgatacttt cttcatcgac ttaatctgag cattaccgcc
     3481 cactcttgat accgaaatac caacgttgat agctggtcga acaccagagt taaacaagtt
     3541 tgactcaagg aatatctgac catccgtgat cgatatcaca ttagtaggta tatatgccga
     3601 tacgtcacct gcctgtgtct cgatgatagg taaggcagta agcgatccac ctccctttac
     3661 aatgcctgca gccttcaatg attccggcag gtcattcata tttcttgcaa tctcgtcatt
     3721 attgataacc ttagccgccc tttcaagcaa gcgtgagtgt agatagaaca cgtcacccgg
     3781 gtaagcttca cgtcctggtg gtcttcttag tagcaatgac acctcacggt aagcaactgc
     3841 ctgttttgaa agatcatcat atactatcaa tgccggacga ccagtatctc tgaagaactc
     3901 tccaatagat gcacctgcaa acggagcata gaactgaaga ggagcagggt cagcagcaga
     3961 agcggataca atcactgtgt aaggcaatgc accattctct tcaagaatac gtgccacctg
     4021 agcaatggta gaagcctttt gaccagatgc tacatatata caatatacag gttctccttt
     4081 ttcgtaaaat tctttctggt taatgatggt atctatggca atagccgact tacccgtctg
     4141 tctgtcaccg atgataagct cacgctggcc tctacctatc ggaatcatcg catctattgc
     4201 tttgataccc gtctgtagtg gctcagtaac cggttgtctg aaaataacac cgggtgcttt
     4261 gcgctcaagt ggcatttcat aagttttacc ggcgatcggg cctttaccat ctataggttc
     4321 gccaagagga tttaccacac gaccgagcat accttcacct acttgaagtg atgcaatctt
     4381 tctcgttctt cgcacggtat ttccttcatt gataccatct gagctaccaa gaagtaccac
     4441 acctacgttg tcttcctcga ggttaagtac aatagcctga acaccatttt cgaattcgac
     4501 aagctcaccg gcttgtacac tgttaaggcc ataaacacga gcaataccgt cacctacctg
     4561 tagtacggta cctacctctt ccaattgctg agcagtactc atgcctgaaa gctgctgctt
     4621 tagaatcgct gaaatttcat ccggttttac atcaaccatg atcgtataaa tttaaatgtt
     4681 tattattaat tctgaatatg caaatttcct gtaaactgtt tctttacttg ctccaactgg
     4741 tattttacac tggcatcgta cagcttatcg tcaaattcta ccaaaaaccc accaataagt
     4801 gccgggtcaa cggtgtgtat gatctcaaca tttgacctgg tactaccggc agcttcaaat
     4861 ttctggcgta tagcatcaat cgtctttgca tctacctcag tagctgtcac cagcctgatg
     4921 gtcgatatat gattgatagc cttatattgc tctatgaatt cgctcgctat ctcaggtaga
     4981 tatgattcgc gacccttgtt gagaataata ttaaaaaaag ccatagtcac ttggtcaacc
     5041 ttaccatcga aaagctcacg gaatattttg gtcttcgttc cggtattgat gatcgggctc
     5101 tgcagacaca tcttgaagtc gcggttcttc gctacttcaa ggaatcccat gacatcttct
     5161 ttaatcctgt ccagcttgtt attgtccctg gccaggtcaa tgagtgattt ggcatatctg
     5221 gtagctactc tttgtacaga catagttttg cttttttaaa aacaagtttt aatttaactt
     5281 gatatcttta accagtgtat tgacataagc ttcctgttca gtattacctt taagttcttt
     5341 cttgattacc ttttcggcga tttctatagc catcattccg gccatgtttt tcacttcagt
     5401 aagcgcagct tttttctggt tttcgatttc gttcattgcc gaagatacta ttttctgggc
     5461 atcttccttt gccttagttc tcgcttcagc tatgatggca tctttcgttt cttttgcttc
     5521 ctttagcatt ttgcttcttt cttcacgagc ttctgtcagt atcttgtcat tctccgcttt
     5581 cagctgctcc atttcttcac gggctttttt cgcctcatca agagcatcct ggatcgaatt
     5641 ttctctcgat ttaagagccg cacctattgg tttgaacgca agctttccta aaatgaacca
     5701 aaatagaagg aatataagtg tcgtccacac caacataccc ggctccggtc tgataactga
     5761 gaaatctgct aagagtatca tattcatttt ataaatattt taagtctttt ttggtttaaa
     5821 ataagccgga aagcaaccaa ccgttgatct tcccggctat cttctttttg ttgtaaccgg
     5881 gtattatcct cctacgaaga aagccaatac catacagata agagctgcac cttctacaag
     5941 tgctgccatc aggatcatgt ttgatctgat gtcacctact gcttcaggct gtcttgcgat
     6001 agcgtccata gctttgccac caacgcttcc gattcctaat cctgctccga ttactgctag
     6061 tcctgcacca attgctgcgt acatgttttt aaaatttata tagttaatta atgatgttct
     6121 tctaccgctg cacctatata agatgctgta agcattacaa atatatatgc ctgaaggaat
     6181 gccaccagca actcgattgt actcataaac aatgtcaggg gtactgatat tgcggcacca
     6241 agtgccgtac caccaatact tgctcccgat ttgccaaata caaatatcaa acccacaaaa
     6301 ctcagtatca cgatgtggcc ggccgttata ttggcaaaaa gtcgcaacaa caacgtgaat
     6361 ggcttgagga acaaactcag tatttcgagt ggcgtaagta tcagtatttt cagaatagcc
     6421 ggtacgcctg gcatccaaag tacgtgcccc caatagtgct tgttgccgtt taggtttgtc
     6481 atcaaaaatg taagcacagc caacaccagc gttaccgata tgctccccgt tacgttggca
     6541 ctacccggaa agaacggaat ctgaccaatg aggttaagcc caagaataaa gaaaaacaac
     6601 gatagcaaaa acggcatgta cttttcatag ctatgcccga tgttcggctt tgcaacatca
     6661 tcgcgtacga aagtgatgaa cggctccata aaactctgca agcctttcgg tgccataccc
     6721 tcacgtcttg tataggcgtt acgcacactg ataaacaata tcagcaacag aacgaataca
     6781 agaatcatgg taaacacatt cttggttatg ctgtaatcat aaaatgacgt aataccacca
     6841 cctacaatac cgccatcaaa agccgatctc gcatctgtct tgtattcctg tccgttatat
     6901 aatgcgtagt gtacttcctt tttcttacca tttacatcct cttccttcac tttgatgccc
     6961 tggatggcca ccgtacctgt agggaaagaa gcatcagcca ctctccttac atttcctccc
     7021 gacagaatgt atccatctac agcgttttcg ccattgccat ggtgttttgg tacaaacgca
     7081 ccagatgaca taatcgtcca gcctttgtcc ggagcataca gtatgcaggg tagtggcagg
     7141 tatgtttcac ctacaagttg aaatacattc gcatcagcta tgtggtgtac cgcaaactca
     7201 ccaggcttaa actcaccctc agcatgggca tcatgtccag tttcagcatc tgcatggccg
     7261 gctgccgttg ccgttggtga ctcattgaca tgttcctggc cttcatgctg tgccatcagt
     7321 ccaaaagagg ccgtaattat caaggaaaaa attaagagaa tccgtcgcat gtgcgtatct
     7381 tgatgtattt ttaagtccag tttttcaaaa tcgctgcaaa tgtaccaata ttcacgatga
     7441 gaacaaagat aattttgttt tttttggtaa aaaataagac gaattttgat tcaaacacaa
     7501 gttcaccacc cgtttcagaa taatcaacgg ttttttaact ccggataaca cattaataat
     7561 tcgaacgaaa atgatccgta caaaaaaatc cataagcata gtgaactatg gtttttaacc
     7621 tgaattatgc aataggcttt caattataac tataattgac taactttg
//
LOCUS       DAOQQY010000116         7572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3877_length_7572_cov_5.655980, whole genome shotgun sequence.
ACCESSION   DAOQQY010000116 DAOQQY010000000
VERSION     DAOQQY010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7572
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_7572_cov_5.655980"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1951)
                     /locus_tag="PLE23_06835"
     CDS             complement(<1..1951)
                     /locus_tag="PLE23_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83013.1"
                     /translation="MLKLLKNITLLTGFFWYGQYLEAQNCNFTCKDTVFAVLDSNCQT
                     LVTADDAILDKGNCSNDFVLKITDFIGKSVSNVFGKNDIGKLYHAHLTHLQSNQPACV
                     SVLKIVDKTGPKMVCQDVFADCRAYSMVTDNVFTPPAPLDNCTQVIGLEHNDKYFDMS
                     CQQKGFTGPFDISRWEKQSLCQSNSKISISALQDSLQLLNGLTPDACITALSFKMPFE
                     GILRMKWSSDPANMTSGDSFGIRVNGIFVRLDTASNITGSVATKLLNKEMLFEWVVYA
                     DGAGQPFMVSITNLESESSLKAFVERRWNAFDAYGNKNSCLQSIFILKNYVDEVFFPV
                     DVTDSIKVQKNCTGNYSPDITGWPYLKTGKYNTSLGIIQIKPGSSECLQVSYTDQTNK
                     ICEGSYEIQRKWKIIENCTGDSIIHDQMIRVLDLAAPIISSPAQLSVPVHDYDCVADV
                     TIPACTATDLCSPNGLTTSITTSFGGSGTGPFADIPTGQYKVTYTAIDGCGNKSSKMI
                     DLEVKDLTAPLAKCKQNLSLYLDGAGEANISASATDNGSTDNCCLTEIKIKRKNDQQA
                     AFVPTLKFTCADAPKVQVALRATDCNGNIAVCDATVTIADNIPPVVVCPGDVTVSCST
                     DLTQLNAYGSPIVTDNCNAKTLYSFT"
     gene            complement(1972..5001)
                     /locus_tag="PLE23_06840"
     CDS             complement(1972..5001)
                     /locus_tag="PLE23_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83014.1"
                     /translation="MNRFFYTFFFIIFTNVLFSQPAFTVKNYSVEQGKPVCCDIVVSG
                     FKDILTAQFTFAWDPAVIKFDSVNNFKLQGLTGNNIGTSKTAQGLLSFSWFDVNASGK
                     SLNDGDVLFTVCYTAVGSAGDESPLWFAGSPIKTEVTDIYSNGEDIGAVTTDGSVKII
                     EQQAAISFQKISLESGSSGCNLLSANGIDAVKNLEGTINWDPAVVAFDSLCCLHPQLS
                     KQYFNELLASSGKILLDYDNAVPIDTKTSPVIAGFCFTAIALPGSSTNITLDGSYFPL
                     NVKNGTNKPLSINEGVISIDLQAYRVILPDVVTKQGETMTVPLLAGVSGKLNAFSGNI
                     CFPAQELQYNLVYKSKIAGLTLDPPVTTNNITTISWNWTDASNATYFAKNDTIISIML
                     KANGPKSSKHNVSLCTGKYPLQATVDINKSYDLNVVTDTGSVFILPDNVRMQMNDSTG
                     KLNEIVCVPVHVRDFKNIRAFEMVFKSDPAIAQPIEIKNKLIPLTPAPQFTVLSDVLS
                     VKWQGVTNNDLLSLVDDAVIVELCYQLIGSVGSITQIATDTSSFYLLGETGSKHFAFE
                     GDTALLTVESGLTVLSVTSTVVDNNCQGEENGNILLNIKGGKLPYKFLWSNGSVTQNI
                     SHLADGFYSVTIMDASVPAQVLHQDFSITHLHQNPSFTDIATQQIDCPGGSVHVQADN
                     PTLFYTWSGNGLLKNGVTSVEISEAGFYTVKGMDQTTSCSVADTFEVKDAPVLEDAFV
                     TESFLYACDNVLLEAVQSPGVTGYWTGAGGNIVSPNQHSTLVEELTPGLQYFVWTISS
                     AACTDYSNDTIWVFVKFPVEANDDVLKKDVKYYNIIANDAINPGQVKLTIHSSLPQGM
                     TIDSFGNLSILPDFSAWDVSVSYTICDPVCTDNCSQGQISFVKDIPDDKDTTVVDPEV
                     PGAKAYILPNAISPNNDGSNDYLFFDEISNGQLVRAHLLVFDRAGRPVFESKDYKNNW
                     SGKHSDGSDLPVDTYYYVLYIDLQKGKYVKGPVTILK"
     gene            complement(5026..5934)
                     /locus_tag="PLE23_06845"
     CDS             complement(5026..5934)
                     /locus_tag="PLE23_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83015.1"
                     /translation="MKKPALAFLVLLYCIHLRAQNLNICPTRTQHLTSELLAAILLYK
                     HNLHSSASTTTRWTGFSQHPLAIHLSGNFLPPSALSGLLFGGELAMESYGPFRNYSFL
                     PSVSVLKTDRNFASGYYAGLKAGFAAGRLISSGLVWRENEPIQYETDGIIKALIAYEA
                     GGWISFDRKRKILMVDFNSHNITDTESKRNVFSRLVSGRYQVKWINGNEESHTETTYE
                     LWLTDLKYITHTASFRYYSPIGLFGGIQLDSNFESACLAGYRWLPTYSEDAFFDFAYT
                     FGYSLARYGPFAGNSHGLSIRYSASL"
     gene            complement(6123..>7572)
                     /locus_tag="PLE23_06850"
     CDS             complement(6123..>7572)
                     /locus_tag="PLE23_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HMX83016.1"
                     /translation="FIFLFLKIYNKYASAQKLNSIHDHSLLTRLWKTPVFLLSGAFLA
                     IVLIRGGLQAIPIQSSDSFFSTTPIVNDVAVNPFWNIAYNILDYQNRFKKNPFNDFQM
                     DEANNYVRSLFQVQKDTTINFLTNDRPNIVFILAESWNTHSIKSFGGDDFAPFIDSLS
                     RQGIRFTNFYSSGYVSDQGIPSVLSGYPSVSRVSVINQNSKSAKLPCINQDLKKYGYQ
                     SGFLFGGDLNYGNIKGYVLNKAFDIVKEEKDIDPKYARGKLGIQDNDMINVLLENLNA
                     AQTPFVFAWFTLSTHMPYDFEGAKKNLVNHKENDYINSIVFSDHALREFFEKAKNTNW
                     YKNTLFVFVADHSHADHKDFGVYDAEYHHIPLIFFGDVIKPEFRGKEIRDVFSQLDIT
                     PTLLKQMGLNTEAGQYVWGRNMFNPYSRHFAYFSTFAGGGVVSDEGKIGYQHGLDKLI
                     INSFGPGNPKSTELKRLGKAFQQTVFEDYRLK"
BASE COUNT         2235 a         1560 c         1545 g         2232 t
ORIGIN      
        1 gtgtaaaaga atatagcgtt ttggcattgc aattgtcggt tactatagga ctcccgtagg
       61 cattgagctg agtcaggtct gtcgaacaac taaccgtcac atcacccgga caaaccacca
      121 caggtggaat gttgtcagca atcgttacag tagcatcaca tactgcgata ttgccattgc
      181 agtcagtagc tcgaagtgcg acttgtactt tcggtgcatc cgcacatgta aattttagtg
      241 tcggtacaaa ggcggcctgt tggtcatttt ttctcttgat ttttatttct gtcagacaac
      301 aattgtcggt acttccatta tcagttgctg atgctgaaat attggcctcg cccgcaccat
      361 ccaggtacaa gctaaggttt tgcttacatt tggcaagcgg tgctgtcaga tctttaactt
      421 cgaggtcaat catttttgac gatttgttac cacatccgtc tatggcagtg taagtcacct
      481 tgtactgacc ggttggaata tctgcgaacg gccccgtacc actaccgcca aacgaagtag
      541 taatactggt agtcaaacca tttggtgagc acaaatcagt tgccgtacat gccgggatag
      601 taacatctgc aacacagtcg taatcatgca ctggcacact aagctgggca ggacttgaaa
      661 tgatcggtgc tgcaaggtca agcacacgta tcatttggtc atgaatgata gagtcgccag
      721 tacagttttc aataattttc catttgcgct gtatttcata gcttccctca catattttat
      781 tggtctgatc tgtataactt acctgtaaac actcgctgga gcccggttta atctgaataa
      841 ttcccaggct ggtattgtat tttccggttt tcagataagg ccatcctgta atgtcaggtg
      901 aataattacc tgtacaattt ttttgtactt tgatactgtc tgttacatca accggaaaaa
      961 atacttcgtc cacgtaattt ttcaggataa atatactttg taagcaggaa ttcttattgc
     1021 cataggcgtc aaaagcattc cagcgtcttt ctacaaatgc cttaagagaa gattcagatt
     1081 caaggttggt gatactcacc atgaacggtt gccctgcccc atccgcgtaa accacccatt
     1141 caaaaagcat ctccttgtta agcagcttgg tagctaccga acccgtaatg ttacttgctg
     1201 tatccaacct tacaaaaata ccgtttaccc tgataccgaa actatctcca cttgtcatat
     1261 tggcaggatc gcttgaccat ttcatcctta gtattccttc aaaaggcatt ttaaaagata
     1321 atgcagttat acaggcatcg ggtgtcagac cattcaataa ttggaggcta tcttgtagtg
     1381 ctgatatgct aatcttggaa tttgactggc agagtgattg tttttcccaa cgggaaatgt
     1441 cgaacggacc cgtaaatcct ttctgttgac agctcatatc gaaatatttg tcattgtgtt
     1501 cgagaccgat gacctgagta cagttatcaa gtggtgcagg aggagtaaag acattatcag
     1561 tcaccattga gtatgctcta caatcggcga atacatcctg acataccatt ttaggacctg
     1621 ttttatcaac aattttcaac acagaaacac atgccggttg attgctttgc agatgggtaa
     1681 gatgggcatg ataaagcttg ccaatatcat ttttcccaaa aacatttgag actgattttc
     1741 ctataaaatc ggtaattttc aaaacaaaat cattgctgca gtttcctttg tcaagaattg
     1801 catcatcagc agttaccaaa gtttgacaat tgctgtctag caccgcaaaa actgtatcct
     1861 tgcaggtaaa attacagttc tgggcctcca gatattggcc gtaccaaaaa aatccggtaa
     1921 gcaatgtgat atttttcaga agtttgagca tgagattgtt gtttttcagg tttatttcag
     1981 gatagtcaca ggacctttta catattttcc tttttgcagg tcaatataaa ggacataata
     2041 ataagtatca accggaaggt cagaaccatc tgaatgtttt ccgctccagt tatttttata
     2101 gtctttgctt tcgaaaaccg gcctgccggc gcggtcaaaa acgagaaggt gtgcgcgaac
     2161 aagctgcccg ttactgattt catcaaaaaa aagataatca ttgctgccat cgttattcgg
     2221 gcttatggca tttggcagaa tataggcttt tgcccctggc acctccggat ctaccaccgt
     2281 tgtatccttg tcatcgggaa tgtcttttac aaatgaaatc tgaccctgag aacagttgtc
     2341 tgtgcaaacc ggatcacata ttgtatatga aacacttaca tcccaggcgc tgaagtcagg
     2401 caaaatgcta agattgccaa atgaatcaat tgtcataccc tgtggcaaac tagaatgtat
     2461 tgtcaatttt acctgacccg gattgatagc atcgtttgct atgatattgt agtattttac
     2521 atcttttttc aagacatcgt catttgcctc caccggaaat tttacaaaaa cccaaatagt
     2581 atcattgcta taatccgtac aagcagcaga agatattgtc catacaaaat attgtaggcc
     2641 tggagtcagc tcttcaacaa gtgtgctgtg ttggttcggt gaaacaatat tacctcctgc
     2701 accagtccaa taacctgtca caccaggtga ttgaacagct tcaagcaaca cattatcgca
     2761 ggcataaagg aaagattctg ttacaaatgc atcttcaagc accggcgcat ccttcacctc
     2821 aaaagtgtct gctacagaac acgatgtcgt ctgatccatt cctttgacgg tgtaaaaccc
     2881 ggcctcagaa atctcaacag aagtaacgcc atttttcaga agaccatttc ccgaccaggt
     2941 atagaacagc gtaggattat ctgcctgcac atgtacactt ccgcccggac aatcaatctg
     3001 ctgagtagca atatccgtaa acgaaggatt ttggtggaga tgcgtaatgg aaaaatcttg
     3061 atgcaacacc tgagcaggca cggatgcatc cataatggta acactgtaaa atccatcggc
     3121 aagatggctg atattttgtg ttacactgcc atttgaccaa agaaatttgt atggcagttt
     3181 accacccttt atgtttaata agatgtttcc attttcttca ccctgacaat tattgtcaac
     3241 gacagttgat gtaacactca gtacagtcaa gccagattca actgtaagta gggctgtatc
     3301 accttcgaaa gcaaagtgtt tgctccctgt ttcgcccaaa aggtaaaaag aagaagtatc
     3361 agtagcgatt tgtgtaatag aaccaacact accaataagc tgatagcaaa gctctacaat
     3421 tacagcatca tcgactaagc taagcaggtc gttgtttgtt actccttgcc atttgaccga
     3481 taatacatcg gagagtacag taaactgagg tgccggtgtc agaggtataa gtttattttt
     3541 tatctctatt ggctgagcaa tagctggatc tgatttgaat accatttcga aggcgcggat
     3601 attcttaaaa tcccggacat gcaccggcac gcatactatt tcattaagtt tacctgtgct
     3661 gtcattcatt tgcattctta cattatcagg tagaatgaat acagaacccg tatcagttac
     3721 tacattaagg tcatagcttt tattgatgtc aaccgtagct tggagcggat atttcccggt
     3781 gcagagtgag acattatgtt tactgctttt tggtccattt gcctttagca tgatagaaat
     3841 aatcgtatca ttctttgcaa aatatgttgc gttagaagca tcagtccagt tccagctgat
     3901 ggtagtgatg ttgtttgtcg tcaccggagg atcaagtgtt aatcctgcaa ttttactctt
     3961 atacactaga ttgtactgaa gttcctgtgc cggaaagcaa atattcccgg aaaaagcatt
     4021 cagtttgccc gacacacctg ccagcagcgg cacagtcatg gtttctcctt gcttcgtgac
     4081 tacatcagga agaatgacac ggtacgcttg aaggtcaata ctgatgacac cttcatttat
     4141 gctgagtggt ttgttggtac cgttctttac attcagcgga aaatatgaac catccagtgt
     4201 gatattggta gatgaaccgg gtagagcaat tgcagtaaag cagaatccgg ctataacagg
     4261 tgatgttttg gtatcaatcg gcacggcatt atcataatca agcaatattt tgcctgatga
     4321 agccagtaat tcgttaaagt attgtttaga aagctgcgga tgtaggcaac agagagaatc
     4381 aaatgctacg acagcggggt cccaattgat ggtaccttca agatttttca cagcatcaat
     4441 accatttgct gaaagcaagt tacagccgga agaaccggat tcgaggctaa tcttttgaaa
     4501 tgaaattgca gcttgttgtt caatgatctt gactgaacca tctgtagtga cagctccaat
     4561 atcttcaccg ttggaataaa tgtctgtcac ttctgtctta atagggctgc ctgcaaacca
     4621 tagtgggctt tcatcgcctg cactaccaac ggctgtatag cagaccgtga aaaggacatc
     4681 accgtcgttc aacgatttgc cggaggcatt aacatcgaac cacgaaaacg aaagtaatcc
     4741 ttgagcggtc ttacttgtgc caatattgtt tccggtaaga ccttgaagtt tgaaattatt
     4801 taccgaatca aacttgataa cagccggatc ccaggcaaag gtaaactgag cagttagaat
     4861 gtctttgaag ccggaaacga cgatatcgca gcagactggc tttccctgct caacactata
     4921 gtttttaaca gtgaaagccg gctgcgaaaa aagcacattg gtaaaaatga taaagaaaaa
     4981 ggtgtaaaaa aaacggttca tgggaatatg tttgttgaag tgggattaaa ggctagcgga
     5041 ataacgaata ctaagtccgt gtgaattgcc ggcgaatggt ccataacgtg caagcgagta
     5101 accaaaagtg taggcaaagt caaaaaatgc atcttcacta tatgtgggaa gccatctgta
     5161 acctgccaaa caagcacttt caaaattgct gtcaagctgt atgcctccaa aaagtccaat
     5221 cgggctgtaa tatctgaatg aagctgtatg tgtaatatat tttaaatccg tcaaccagag
     5281 ttcgtatgtt gtttcggtat ggctttcttc atttccgtta atccatttta cctgatatct
     5341 gcctgatacg agtcgtgaaa atacatttct tttactttct gtatctgtga tattatgcga
     5401 attaaaatcg accatcaaaa tctttctttt tctgtcaaaa ctaatccaac caccagcttc
     5461 ataagcaatt aatgctttaa ttatgccatc agtttcgtat tgtatcggtt cattttcacg
     5521 ccatacaagt cctgatgaga ttagccttcc ggcagcaaaa ccggctttta accccgcgta
     5581 atagcctgat gcaaagtttc tgtctgtctt tagcacactt acggaaggaa ggaagctata
     5641 attcctgaat gggccatagg attccatggc caattcgcct ccgaacaaca atccacttaa
     5701 tgcagatgga ggaagaaaat tgccggaaag atgaatggct aaaggatgct gactgaagcc
     5761 tgtccaccga gtagtggtgc tggccgaaga atgtagattg tgtttatata ataatatggc
     5821 ggctaaaagc tcggaggtaa gatgctgtgt ccgggtcgga cagatgttca ggttttgtgc
     5881 gcgtaaatga atgcaataca atagcaccaa aaacgctaag gccggttttt tcatgggatg
     5941 tatgttcggt ttctttgtaa atacatggca aagatgcaaa caagaatctt ttctgtcaat
     6001 accctgaaaa tgtatttatt atatataaat attactaata atacaaaata aaatacatga
     6061 aatactcatt gttatatgaa aatgaaaata taactacaga tttcaccaga aaaataaaaa
     6121 aattatttta gcctataatc ttcaaaaact gtctgctgaa atgcttttcc caaccttttt
     6181 agttcggtcg attttggatt tccgggtccg aacgaattga ttataagctt atcgaggcca
     6241 tgttggtacc ctatttttcc ttcatccgac acaacacctc caccagcaaa agtgctaaaa
     6301 tatgcaaaat gtcttgagta aggattgaac atatttctac cccacacata ctgcccggct
     6361 tcagtgttaa gacccatctg ctttagcaat gtaggtgtaa tatctagctg cgaaaataca
     6421 tctcggattt ccttgccgcg gaattccggt ttgatcacat caccgaaaaa aataagtggt
     6481 atgtggtggt attcggcgtc atagactccg aaatccttat gatcggcatg actgtgatct
     6541 gctacgaaga caaagagtgt attcttgtac caatttgtgt tttttgcttt ttcgaaaaat
     6601 tcgcggagtg catgatcaga aaaaacgatg gaattgatat agtcgttttc cttgtgattc
     6661 acgagattct ttttcgcacc ttcaaaatca taaggcatgt gcgtactgag cgtaaaccag
     6721 gcaaagacaa atggtgtttg ggcagcattc agattttcca acaacacatt tatcatatca
     6781 ttatcctgga taccgagctt accacgcgca tatttcgggt caatgtcttt ttcttccttc
     6841 actatatcaa aagctttgtt gagtacgtaa cctttgatat tgccataatt aaggtcgcca
     6901 ccgaacagga atccgctttg atagccgtat tttttcagat cctgattgat acatggcaac
     6961 ttagctgatt tagaattttg attgatgaca ctcaccctcg atacagacgg ataaccgctc
     7021 agaactgaag gtatcccctg gtcagataca tagcctgaag agtaaaaatt agtaaaccgg
     7081 ataccttgtc tgctgaggct gtcgataaaa ggtgcaaaat catcaccacc gaaagatttt
     7141 atgctatgtg tattccaact ttctgcgagg ataaacacga tgttaggcct gtcattcgtg
     7201 agaaaattta tggtagtatc tttttgtact tgaaagagcg accgtacata attattggcc
     7261 tcatccatct gaaaatcgtt gaatggattt ttcttgaagc ggttttgata atcgaggata
     7321 ttgtaggcga tgttccagaa cggattgaca gcaacatcat taacgatagg tgtagtagaa
     7381 aagaacgaat cactactttg gatagggata gcctgaagac cgcctcgtat aagaacaatt
     7441 gccaaaaaag caccggacaa caagaaaacc ggagttttcc agaggcgggt gagtagtgag
     7501 tggtcatgta tggagttcag tttttgagca gaggcatatt tgttgtatat tttcaggaac
     7561 aaaaagataa at
//
LOCUS       DAOQQY010000117         7541 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3902_length_7541_cov_5.609404, whole genome shotgun sequence.
ACCESSION   DAOQQY010000117 DAOQQY010000000
VERSION     DAOQQY010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_7541_cov_5.609404"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLE23_06855"
     CDS             <1..256
                     /locus_tag="PLE23_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMX83017.1"
                     /translation="SKHEYTFTNTGKEPLIISDAKGSCGCTVPTWPKDPIAPGKTGVI
                     HVEFNSKGKLGPQSKKVTLTANTNPAQTFLTIKGEVTPVK"
     gene            651..5654
                     /locus_tag="PLE23_06860"
     CDS             651..5654
                     /locus_tag="PLE23_06860"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83018.1"
                     /translation="MKVKVHLRLIVCLVFFANLSFIQGYTPSYFNPDTIHLPSELFKQ
                     TNDCTAKLDLCLDDLANPGDLQITVNDSVYTGSIANCQEDSSFIYTLFTLYGFGKLGP
                     YHLDKWEVNGQVFSGFFQNPDELLILMNNWDPAGNWTYDPNTFLISGGKSGSDYSEMD
                     VTVLSLGGAKGYIGLNIGINGTGVKIFLETGKHHIIFTNTITNVSDTVDLQYVCVKPS
                     VVYNTVQKSKSDVYCLDASELTGPIVSVINICPDHSGSNVTYSILANNCIQFTGLEVG
                     VDTFCAKYCDANGICDTTTIITKVTPPTGSNDIVINIFELTDTLHCFDISQLPGNAVS
                     MVNICPDAADGDVDFYTNNVDYCVFLTGLSVGSDTACYVICTDSSYCDTFKLIVNVLP
                     LPKADTVYLKVKEKNSIVYCLDLSELPNGYKSIENICPNAAGDNAKISFDFASACMGI
                     KGLKAPGKDTACYVVCDASNICDTIIVIIDVYKDPNPPKVIDITIEYNEVISYCIDTT
                     QLSGNLVSITNVCPDKSGVNADVAPDPATYCVYALGILPGVDSACIVVCTDAGICDTT
                     IIIYHVVEHTDPTEIIVDVVEGTTIQYCIDQYLVCTPIISVQNFCPDKLFDNSEITPG
                     NDPLCFNIAGINPGGKDTLCLNICCDFGNCDTVFLIINVLPASHHKLIQLTVKEEEFL
                     KYCIDPNTVCSPILSVQNICQGSAGTYAQIAYEPNTGCVLIDGLEAGGTDSICLVFNC
                     GNVTDTVIVAVHVIKDGTKEVVNLSVPEGETMVYCLDPTALCSPILSIENICPGASGE
                     NAIIDFDASTGCAFIQGIKANGADSLCLVIKCAGQSDTVIVIVQVTPPVNKNLKEITI
                     KVGDEILYCFDEKELCSPILSVENICPDASGENALIQLDPYNICAKITGFKQGDDSLC
                     LVVNCGTTSDTVTLIVHVLPYKNNQTVEVTVEKGKTLEYCFTEAGVCMPVLSIENICG
                     NTVFDNSSIVFDSITLCAKIKGVAAPGTDTLCLAFCCGPNKCDTVTLLIHVIEKNNPP
                     LVVNLTIDVTDTIQYCLDLSDLSTGVASIQNLCPQQSGNYTQSSFDPVTNCFTFIGVQ
                     PFGQDIFCMVACDSTGFCDTTIIFVNVIPKIITPETVNLTVYEGATVKYCLDTTEIGS
                     AIVSFINFCPQSMGQNASMSYDSNTHCIEFKGIQAFGTDTACIVICGSNGFCDTTTII
                     VQVVKKQFPDTLYFNVKEGGSLQHCFDPDLIGGNISSIVNICPQQSGTNAAYFYLLTD
                     TCVTIVGIAVGGPDTACFVICNNLGYCDTVTLITNVYKDDVKIVYDTILVHFKDTLCF
                     DISGFDPNTIKIENICPDESGTFVDFTISGDTACVYYTGLGIGTDTACIKISDADSNS
                     LLTHIIVTVIPPAPDIITDIIHVDSTKVYCLDTTELAGNVLSVVNICPDQSSGNISYT
                     INEQTACVTVTGLVPGKDTLCLVYCDTFGICDSTTIFITVIENNANPIAVDDSVSTNI
                     NVPVVINVLGNDVNPGGAGVVTILPPSLGGTGPNHGTATVNADGSITYIPDDGYCGED
                     IFTYLLCIGQNCDPAVVIINIECPPNQTDTLEVFNGFSPNGDGKNDVFTIKNIEKFPD
                     NKLYIFNRWGNRVYFEEKYLNTWEGTWENKPLPDGTYFYIIEDGKGKTYSGYLVIYR"
     gene            5714..6703
                     /locus_tag="PLE23_06865"
     CDS             5714..6703
                     /locus_tag="PLE23_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMX83019.1"
                     /translation="MKKLLIAFLFLYAGNVFSQQDPMFTKYMFNSLWYNPAYAGSQDH
                     MSISLIHRSQWIGLQGAPTTQTFTIHTPLRNDKVGIGFNLVNDKIGPTNTLLANLAYA
                     YHIPVGFGRLSFALQVGVNNFRADWSKLTIQDPDPNFEVTPNKWLPNFGAGVYYYTNR
                     FYAGLGVPHLIEYDLSPKEVDNDLYAKQYRHYYFSMGGAINVNGPDVVFKPSLLIKNV
                     GLLSKYSKEVAFQSVTAPTEFDIDLSFLFYQSLWVGTAFRSSVEAFDNDRSTYDSWDV
                     WASYLMSNGLRIGVAYDYSLPKQLRAINDGSFEVMLGYEFDYKTNKIVTPRYF"
     gene            6736..>7541
                     /locus_tag="PLE23_06870"
     CDS             6736..>7541
                     /locus_tag="PLE23_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83020.1"
                     /translation="MSVRKVILIVMSIVMLASVAEAQRADLKRAGKMMKNLEYKEAIR
                     IYNKVLEKRDNAEAKINIAEAYRKIDDAENAEYWYGQVVILPEAQPIHQLYYGEALQK
                     NGKCDKAKEYFKKYNEAVPGDVRGKQLLAACDMQEDLMNKNGDIYTIDYLPFNSNLDD
                     FGPVFYKGALVFASERDKGYAVKREHTWTGLPFLDLFQVDSKDLNNKTCTPLAFGKPK
                     KFNASLNTKYHDAAVAFNSDYSEIYFTRNNYLSGKTGKSDENIVNLKIYTA"
BASE COUNT         2229 a         1507 c         1520 g         2285 t
ORIGIN      
        1 atctaagcac gaatatactt tcaccaacac aggtaaagaa ccgctcatca tcagcgatgc
       61 aaaaggtagc tgtggatgta ctgtaccaac ttggccaaaa gaccctatcg ctccgggtaa
      121 aacgggtgtt atccacgtag agtttaactc taagggtaaa ttggggccac aaagcaagaa
      181 agttaccctt actgcaaata ccaatcctgc acagactttc ctaaccatca agggtgaggt
      241 gacgcctgtt aaataaattt ttagagaata attttcttca gaatggtctg tgtaacaaat
      301 agttgcacag accattctgt tttattacat tgtgtgtcct acttatttga tgtagcactg
      361 tttgcttaat gtgctttttt tatttgtgac atttagatta aaattgcgtc agattattat
      421 agttaatagt tttttgtgta acatttttct ttctaaattt attgtttttg tcttcaatgc
      481 ttattctgaa atgctcattc atccttattt cagtcttttt caagctttta gatagcctga
      541 atgtagatgg aatcattttg gcataatttt agagaaggtt taattatatt ttttttacat
      601 ataaaaaaat aaagacaaat tttgtccctg acattccgta aataccgatt atgaaagtaa
      661 aagtacatct aaggttaata gtatgcttgg tattttttgc taacctgagt tttatacaag
      721 gttatactcc ttcttatttt aaccccgata ccattcattt accttctgag cttttcaaac
      781 agaccaatga ctgtactgca aagctagact tgtgtctcga tgatcttgcc aaccccggcg
      841 atctacagat tactgtgaac gacagtgtat ataccggctc aatcgctaat tgtcaggaag
      901 actcaagctt catctacaca ttgtttactt tgtacggatt tggaaaatta ggtccgtatc
      961 atctcgataa atgggaagta aatgggcagg ttttttccgg ttttttccaa aacccggatg
     1021 aactcctgat tttaatgaac aactgggacc cggctggcaa ctggacttat gaccccaata
     1081 ccttcctcat ttccggaggt aaatcgggct ccgattattc agaaatggac gtgacagttc
     1141 tatcccttgg tggtgcaaaa ggttatatcg ggcttaatat cggcattaac ggaacaggag
     1201 tgaagatctt tttggagaca ggcaaacacc atatcatttt tacaaatact ataacgaacg
     1261 tttcagacac agtcgatctc caatatgtat gtgtaaaacc ttctgtggta tataatactg
     1321 tacaaaaatc taaatcggac gtttactgcc ttgatgcaag cgaacttacc ggtccgattg
     1381 tatctgttat taatatttgt ccggatcata gtggcagtaa tgtcacctac agtatattag
     1441 caaacaattg tatccagttt accgggctcg aagttggggt agataccttt tgtgcaaaat
     1501 attgtgatgc caatggtatc tgtgacacta ctaccattat tactaaggta acacctccta
     1561 caggttcgaa tgatattgtt atcaatattt ttgagctcac agacacactt cactgttttg
     1621 acatttcaca acttccgggc aatgctgttt ctatggtaaa tatctgtccg gatgcagccg
     1681 atggcgatgt tgatttttat acaaataatg ttgattactg tgtattcctg accggcttat
     1741 cggtcggttc tgatactgcc tgctatgtta tatgtacaga ttcgtcgtat tgcgacacat
     1801 tcaagcttat agtaaatgtg ctgccattac caaaagctga tacggtttat ctgaaggtaa
     1861 aggaaaaaaa ctcgattgta tattgtctcg atcttagcga attgcccaat ggctataagt
     1921 caattgaaaa tatttgcccc aatgcagcag gtgataatgc aaaaatctct tttgacttcg
     1981 cctcagcctg tatgggcatc aagggtttga aagcacccgg taaggacact gcctgttatg
     2041 tggtatgcga tgccagcaat atatgtgata ccattattgt catcatcgat gtttacaagg
     2101 atccaaatcc gccaaaggtc atagatatta ccatcgaata caatgaagtt atttcttatt
     2161 gtattgatac aacgcaactt tcgggcaacc ttgtttctat cacgaatgtg tgtcccgata
     2221 agtcaggcgt taatgccgat gtggcacctg atccggctac ctattgcgtg tatgctttag
     2281 gaatattgcc aggtgtcgat tctgcctgta tcgttgtttg tacggatgcc ggcatctgtg
     2341 acactaccat cattatctat cacgtggtag aacacaccga cccaactgaa attattgttg
     2401 acgttgtaga aggtaccact attcaatatt gtattgatca gtatttggtt tgtacaccaa
     2461 ttatttcagt acaaaacttc tgccccgaca aattatttga caattcggag ataacacccg
     2521 gtaacgatcc actgtgtttt aatattgccg gtataaatcc gggcggtaaa gatacattgt
     2581 gcctgaatat ctgctgcgat tttggcaact gtgatacagt tttcctaatc atcaatgtac
     2641 tgcctgcttc gcaccataaa ctcatccaac tcaccgttaa ggaagaagaa tttttaaaat
     2701 attgtataga ccctaatact gtttgttcgc ctatcttgtc tgtacagaat atttgccagg
     2761 gttctgcagg tacttatgcg cagatagcct acgaaccaaa taccggctgc gtactcatag
     2821 atgggctcga agcgggtggc acagattcta tttgtctggt gtttaattgt ggcaatgtga
     2881 ccgatacggt aatagtagct gttcatgtca ttaaagacgg taccaaagaa gttgtaaatc
     2941 tatccgttcc ggaaggggag acaatggttt attgccttga cccgactgct ttatgttcac
     3001 ctattctgag tattgaaaat atttgccctg gtgcttccgg agaaaacgcc atcatagatt
     3061 ttgacgcatc taccggctgc gcatttattc agggaatcaa agcaaatggg gcagactctc
     3121 tctgcctggt aattaaatgt gcaggacagt cggatacagt catagtcatt gtgcaggtaa
     3181 caccacctgt aaataaaaac cttaaagaaa taaccatcaa ggttggtgat gaaatactgt
     3241 attgttttga tgaaaaagaa ctctgctcac caatactttc tgttgaaaat atctgcccgg
     3301 atgcttcggg tgaaaatgca ctcatccagc tcgatccata taatatttgt gctaagatta
     3361 ccggtttcaa acaaggcgat gattcacttt gcctggtagt caattgtggt acaacatccg
     3421 atacggttac acttatcgtc catgtattgc cttataagaa taatcagact gtagaagtaa
     3481 cagtcgaaaa aggtaaaacg cttgagtatt gttttacgga agccggtgtt tgtatgcctg
     3541 tgctttctat tgaaaatatt tgtggaaata ctgtttttga taattcttca atagtatttg
     3601 acagcattac gctatgtgct aaaataaaag gagtggcagc cccgggtaca gatacacttt
     3661 gtctggcatt ttgctgcgga cctaataaat gcgatacagt cacattgctc attcatgtga
     3721 tagaaaagaa caatccgcct ctagtggtta atctgaccat tgatgtcacc gacaccattc
     3781 agtattgtct tgacttgtct gatctttcta caggtgtggc aagcattcag aatctatgcc
     3841 ctcagcaaag tggcaattat acacagtcca gctttgatcc tgttaccaat tgctttactt
     3901 tcatcggtgt acagcctttt ggtcaggata tcttctgtat ggtggcttgt gacagtacag
     3961 gtttctgtga tacgacaata atatttgtaa atgtcattcc aaaaataata acacctgaaa
     4021 cagtcaatct tacagtatat gaaggcgcta cagtaaaata ttgcctcgac actacagaaa
     4081 tcgggtctgc tattgtgagt tttatcaatt tctgcccaca gtcaatgggg caaaatgctt
     4141 cgatgagcta tgatagtaat acgcattgta tagagtttaa aggcattcag gcatttggta
     4201 cggatactgc ctgcatagtt atctgtggct ccaatggatt ctgcgatact actactatca
     4261 ttgtccaagt cgtaaagaaa caattccccg acacactcta tttcaatgtg aaagaaggcg
     4321 ggtcactcca gcattgtttt gatcctgacc tcataggcgg taacatttca tctattgtca
     4381 atatctgccc tcaacagtcg ggtaccaacg cagcttattt ttacctgctt acagatacct
     4441 gtgtgaccat agtaggtatt gcagtaggtg gacctgatac agcatgcttt gtcatctgca
     4501 ataatctcgg ttattgcgat acggtaacat tgattaccaa tgtctataaa gatgatgtca
     4561 aaatagtgta tgatacaatt cttgtacatt ttaaggatac tttatgcttt gatatcagtg
     4621 gttttgatcc taatactata aagattgaga atatctgtcc ggatgaatcg ggcacgtttg
     4681 tagattttac catttcgggc gacaccgcct gtgtgtatta tacaggccta ggcattggta
     4741 cggatacagc atgtattaaa atatcagatg ctgacagtaa ttcgttgcta acgcacatta
     4801 tcgttacggt cattccgcct gctccggaca ttattacaga catcattcat gttgacagta
     4861 caaaagtata ttgtcttgat acgaccgaac tggctggcaa tgttctaagt gttgtaaata
     4921 tttgtcctga ccagagtagt ggcaatatat catataccat caacgagcag acggcttgtg
     4981 ttactgttac cggacttgtg ccgggcaaag acacactgtg cttggtatat tgcgatacct
     5041 ttggcatctg cgactctact accattttca taacagtcat cgaaaacaac gcaaacccga
     5101 tagcagttga tgattcggtg agtacgaata ttaatgttcc ggtagtcatc aatgtgcttg
     5161 gcaatgatgt aaatccgggt ggtgccggtg ttgttacgat attgcctcca tcattgggcg
     5221 gtacaggtcc aaaccacgga acagcgacag tcaatgctga tggtagcatt acctacatcc
     5281 cggatgatgg ttattgtggc gaagatatat tcacatactt gttgtgcatt ggacaaaact
     5341 gcgaccctgc tgtggtgata atcaacatag aatgcccgcc taatcaaaca gatacgcttg
     5401 aggtatttaa tggattttca ccgaacggtg acggaaaaaa tgacgtattc accataaaga
     5461 atattgaaaa attccctgac aacaaactct acattttcaa cagatggggc aacagggttt
     5521 attttgaaga gaaatatctg aatacctggg aaggcacatg ggagaataag ccactgccgg
     5581 acggtacata tttctacatc attgaagacg gaaaaggcaa aacatactca ggatatttgg
     5641 taatttatag gtaaagattt aaattgaccc aaattatgta tttgattaac aacgaattaa
     5701 aaaaagacat caagtgaaaa aattattaat agcattcttg ttcctttatg cgggcaatgt
     5761 tttcagccag caggacccga tgtttaccaa atatatgttc aacagtcttt ggtataatcc
     5821 ggcttatgca ggttcgcaag atcacatgtc gatttccctc attcaccgat ctcagtggat
     5881 tggcctccaa ggtgcaccta ctacccagac ttttaccata cacacaccat tacgaaatga
     5941 taaggttggt attggtttta accttgtgaa cgacaaaata ggcccgacca acacactact
     6001 ggctaatctg gcatacgctt accacatacc ggttggtttt ggtcgattgt cctttgcatt
     6061 gcaggtaggt gttaataatt tcagagccga ttggtcaaaa ctaaccattc aggatcctga
     6121 tccgaacttt gaagttacac ccaacaaatg gctgcctaac tttggagcag gcgtatatta
     6181 ttataccaac agattctacg ccggccttgg tgtgccacat ctgatagagt acgatctatc
     6241 tccgaaagaa gtagataatg acctgtatgc aaaacaatac agacactatt atttctcaat
     6301 gggcggagcc attaatgtca atgggccgga cgttgtcttc aaaccatcat tgcttattaa
     6361 aaatgttggt ctgctaagca agtatagcaa agaagttgct ttccagtctg tgactgctcc
     6421 aacagagttt gatatcgacc tttcattctt gttctatcag tcgctgtggg tgggtacagc
     6481 attccgttcg tctgtagagg catttgataa cgatagaagt acatacgact catgggatgt
     6541 atgggcgagc tacctgatga gcaacggcct ccgcatcggt gttgcttatg attattcact
     6601 acccaaacag ctacgtgcca tcaacgatgg ttcattcgaa gttatgctcg gatacgaatt
     6661 cgactacaag acaaataaga tcgtaacacc aagatatttc taaatattcc acgaacattc
     6721 cataaaccat tgaatatgtc agtcagaaaa gtaattttaa tcgtcatgag catcgtaatg
     6781 cttgcgtctg tagcagaagc ccagagagca gacctgaaac gtgccggaaa aatgatgaaa
     6841 aatcttgaat acaaggaagc aattcgtatt tacaacaaag tactggagaa acgcgataat
     6901 gctgaagcaa aaataaatat tgcagaagct taccgcaaaa tcgacgatgc tgaaaatgcc
     6961 gagtactggt acggacaagt ggtcatccta cctgaagctc aacccattca ccagttatat
     7021 tacggtgagg cattgcagaa aaatggcaag tgcgataaag caaaggaata tttcaaaaaa
     7081 tataacgaag ctgtacctgg tgatgtcagg ggcaaacaat tgcttgcagc atgtgatatg
     7141 caggaagacc tgatgaataa aaacggtgat atttatacaa tcgactattt gccatttaat
     7201 tctaacctcg atgattttgg tccggttttc tataaaggtg ctcttgtgtt tgcctccgag
     7261 cgcgacaaag gatatgctgt caaacgcgaa catacctgga cgggccttcc ttttctcgac
     7321 ttgtttcagg ttgactcaaa agacctcaac aataaaactt gtacacctct tgcattcgga
     7381 aaacctaaaa aattcaacgc atcactcaat actaagtatc acgatgctgc ggtagcattt
     7441 aatagtgatt attcagagat atattttaca cgcaataatt atttgtctgg caaaaccggt
     7501 aagagcgatg aaaatatcgt aaacctgaaa atctatactg c
//
LOCUS       DAOQQY010000118         7538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3906_length_7538_cov_6.181478, whole genome shotgun sequence.
ACCESSION   DAOQQY010000118 DAOQQY010000000
VERSION     DAOQQY010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3906_length_7538_cov_6.181478"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..899
                     /locus_tag="PLE23_06875"
     CDS             9..899
                     /locus_tag="PLE23_06875"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HMX83021.1"
                     /translation="MKNVVVIGAGTMGNGIAHVFAMNGFNTTIVDISDVALNKALATI
                     TKNLDRMIAKGSLSEEQKQTTLANLSTSVTMENAVPQADLVVEAATENIDLKLNIFKN
                     IDALVPAGTILASNTSSISITKIASVTNRPDKVIGMHFMNPVPVMKLVEVIRGYATSD
                     EVTETIMTLSLALGKSPVEVNDYPGFVANRILMPMINEAIYTLYEGVAGVEEIDTVMR
                     LGMAHPMGPLHLADFIGLDVCLAILQVMHNGFGNPKYAPCPLLVNMVTAKKLGVKTGE
                     GFYVHTAGSKDLMVSPNFKK"
     gene            complement(911..1300)
                     /locus_tag="PLE23_06880"
     CDS             complement(911..1300)
                     /locus_tag="PLE23_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HMX83022.1"
                     /translation="MSEAKVRIDKWLWAVRIFKTRSISTDNIRSGKVSINKEHIKASY
                     HVKTGDVYTVRKNGFDFQYQVLEIIDKRVNAQIAATCYKDLTPAEELQKFESWYLAGK
                     KGEFREKGTGRPTKKDRREIDDFKDFG"
     gene            complement(1300..2037)
                     /locus_tag="PLE23_06885"
     CDS             complement(1300..2037)
                     /locus_tag="PLE23_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase"
                     /protein_id="HMX83023.1"
                     /translation="MIKINKISTKPPSDLDKDKCKSKTESLAKKIGELQRIIYAEKKS
                     SVLIIFQGLDASGKDGTTRHVLGECGIAGTDSYSFKKPTDDESAHDFLWRVHKMTPAK
                     GMFMIFNRSHYEDVLIQRVHNWIDEDRVAVRMNAINAFEKLLHKDNDTLVLKFFLHIS
                     QEKQLEKLQERIDIPKKNWKHNPADWEEAKLYDKYMDAYEDVINRSELPWHIVPCDKR
                     WYRDYFIASTIHDSLKALNPKLPHIKN"
     gene            complement(2041..2967)
                     /locus_tag="PLE23_06890"
     CDS             complement(2041..2967)
                     /locus_tag="PLE23_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMX83024.1"
                     /translation="MNKFFDNQQIREFSNLELLANQVVEGFIIGLHKSPFHGFSVEFA
                     EHRIYNQGESTKNIDWKVFARTDKLFSKKFEEETNLRCHIVLDASSSMYFPTKEKQGD
                     LDKMRFSILATAALMNLLKRQRDAFGLTIFDSEIRTHTADKSSGQHYQLLLSYLEKLL
                     QEGKHGKSTSAAATLHQVADSIHKRSLVIIFSDMFENISEADELFSALQHLKYAKHEV
                     VLFHVSDKSKELEFEYENRPYLFVDLETGEKIKLQSNEVKSFYKENMQKYLHQLKLKC
                     MQFKIDLVEADINEGFKPILQSYLLKRSKMSV"
     gene            complement(3079..3396)
                     /gene="trxA"
                     /locus_tag="PLE23_06895"
     CDS             complement(3079..3396)
                     /gene="trxA"
                     /locus_tag="PLE23_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMX83025.1"
                     /translation="MAFEFTDDNFQDSALNGGVSVVDFWAEWCGPCRMITPIIEDLSK
                     DYDGKATIGKLNVDDNPNVSMKYGIRSIPTILFIKNGEVVDKQVGVTTKQAMVQKLEA
                     LLN"
     gene            3541..4545
                     /locus_tag="PLE23_06900"
     CDS             3541..4545
                     /locus_tag="PLE23_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 family protein"
                     /protein_id="HMX83026.1"
                     /translation="MNSKLITLLFIIFTVIFLRQFPQFIERYYSDSIFLVIAGSLQSI
                     SSVLRFPLFFLVLAIILYYLLNPQFYKKPGTMVHIISWCSYIIAFFSLSWGLNYARTD
                     ISEKLSFNPINLQDTVLSRLLTEASQELNTTAEMIGRDTPRELEQEYLEDSIPMLVRG
                     EMQELGYRVPKSVVLKNFIPEGWLLRFGTAGFYFPFTAECYIDSGLHPIQKAFVAAHE
                     CAHGYGIGDEGTCNFIAYLACIRSDKLLVKYSAQFAYWRTIKAMAVNQHILKEKLLND
                     IALGDLKAVIEKMKKYPDVFPAARDFLYEHYLKLQGIKEGEANYDRFIELLVQYKNKQ
                     "
     gene            complement(4580..5422)
                     /locus_tag="PLE23_06905"
     CDS             complement(4580..5422)
                     /locus_tag="PLE23_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83027.1"
                     /translation="MKLLISIITTILLMVQATLATAQNVTGTLNNASSNALGGVSSSF
                     ADVRGLAANPASIASIKNMQALIFADQRFGIKELSAFSAGIALPTHSGSFGLSLGRFG
                     FDLYNENLISASYGRKLFSNLSVGGSIQYQYFNIPDYGKKGVVGFQLGLYSELNRRIS
                     LFFSVSNPGRQKITDNDHLPGIFTVGAMYTPNDRLAIRGEFCKTLEHSEDFRFGVEYA
                     PSSRVIIRVGGQTAPAQFGVGFGIVVLETLVVEASAKYNPQIGYHPGVGLSYGIAFDH
                     KKKL"
     gene            5576..6349
                     /locus_tag="PLE23_06910"
     CDS             5576..6349
                     /locus_tag="PLE23_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83028.1"
                     /translation="MVFVIGLVGFIIYARHNHIVPFDKMPLKKAREEAVISNMIEDDF
                     YHFEAEIPNTIRLGGGNSIIVYDTTAGKVISHNQLINVKTDFNHFFEGFEKNKAKKEL
                     DSFNIALVDFSPNEPYFNLLKTNVSRGFYLGIYRELIENIQSWQKRYNSVIVLSKIFP
                     REDGSSDLLILLHSYLKKRNNSPEKQLKAEVAFLIPCRSTTQHLLDFVLPKDFNGSAD
                     VKHFLKMFHYSYPDIKTVRKYWKFDPKRFELRNLKWNFD"
     gene            6358..>7538
                     /locus_tag="PLE23_06915"
     CDS             6358..>7538
                     /locus_tag="PLE23_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HMX83029.1"
                     /translation="MPNRDSFINNIQQGYSFASPSIMLGAANFEGEIIKETQISIPLK
                     TLNRHGLISGATGSGKTKTLQILAEQMSKYGIPSLLMDIKGDLSGIAMPESTNTKIAE
                     RSAQVGIPFAPEGNHVEFLTISDEAGTRLRATVSEFGPALFSKILELNDTQSGLVSVI
                     FKYCDDYRLPLLDLSDMKAVLQHITSDGKEQFAQEFGVVSTVSAGIILRKIIELEQQG
                     AMKFFGEISFEVSDLVRQDENGNGIISIVRLTDIQDKPKLFSTYMLQMLAEIYSSFPE
                     EGDLDKPKLAIFIDEAHLVFQEASKVLLSQIETIIKLIRSKGVGVYFITQNPTDIPDA
                     VLSQLGLKVQHSLRAFTAKDRKNINLAAQNYPLTEYYDVAALITNLGIGEAIITALNE
                     KG"
BASE COUNT         2298 a         1441 c         1446 g         2353 t
ORIGIN      
        1 agctaataat gaagaatgta gtagtaatag gtgcaggcac aatgggaaac ggtatagcac
       61 atgtttttgc aatgaatggt tttaatacaa caattgttga tatctctgat gttgcactca
      121 acaaagcctt agccactatt acaaagaatc tggacagaat gattgcaaaa ggcagtttgt
      181 cagaagaaca gaagcaaact accttagcca atctttccac ttctgttaca atggagaatg
      241 cagttccaca ggccgacctt gtggtagagg ctgctaccga aaacattgac ttaaaactaa
      301 acatcttcaa aaatattgat gcacttgttc cggcaggtac cattcttgct tcgaatacat
      361 cttctatatc aattacaaaa atagcatctg tcaccaacag gccggacaaa gttataggta
      421 tgcacttcat gaacccggta ccggtgatga agcttgtaga ggtgattcgt ggttatgcta
      481 caagtgatga agtaacagaa accattatga cactttctct tgcacttggt aagtcgccgg
      541 tagaagtaaa tgattacccg ggttttgtag ctaaccgtat ccttatgccg atgatcaatg
      601 aagctatata cacactgtac gaaggcgtag caggtgtaga agaaatagat accgtcatgc
      661 gacttggcat ggcacacccg atggggcctc tacatcttgc tgattttatc ggactggatg
      721 tgtgtcttgc aatattacaa gtaatgcaca atggctttgg caatccaaag tatgcaccat
      781 gtccgttatt ggtaaatatg gtgacagcta aaaaacttgg cgttaagaca ggagaaggat
      841 tctatgtcca taccgcaggt tcgaaggatc ttatggtatc tcctaatttt aaaaaataag
      901 tcaggctcat ttacccaaaa tctttaaaat catctatctc acgtctatct ttcttagtag
      961 ggcgacctgt acctttttcg cggaattctc cttttttacc agccagatac cagctttcaa
     1021 acttctgtag ttcttctgcc ggggttaggt ctttgtagca agtagcagct atctgagcat
     1081 tgacacgttt atcgatgatt tcgagcacct ggtattgaaa atcgaagccg tttttcctta
     1141 ctgtataaac atcaccggtc tttacatggt aagacgcctt tatgtgttct ttattaattg
     1201 atactttacc tgaccgtata ttatcagttg atatagaacg tgttttaaat attcgtacgg
     1261 cccacagcca tttgtcaatc cttactttcg cttcactcat caattcttta tgtggggcag
     1321 ttttggatta agtgccttca acgaatcatg tatggttgaa gcaataaaat agtcgcgata
     1381 ccatcgcttg tcacatggta ctatatgcca aggcaattct gatcggttga tgacatcttc
     1441 gtaagcatcc atgtatttat catagagttt tgcttcctcc cagtcggcag gattgtgttt
     1501 ccagttcttt ttcggtatat ctattctctc ctgaagtttt tcgagctgtt tttcctgaga
     1561 aatatgtagg aaaaatttaa ggacaagtgt atcattgtct ttatgtagta atttttcaaa
     1621 tgcattgatg gcattcatac gcacagcaac ccgatcttca tctatccagt tatgaacacg
     1681 ttggatgagc acatcttcgt agtgagacct gttgaatatc ataaacattc ctttggctgg
     1741 cgtcattttg tgtactctcc acagaaagtc atgcgcactt tcatcatctg taggtttttt
     1801 gaacgaatag ctatcagtgc cagctatacc acattctccg agaacatgcc gagtagtgcc
     1861 atctttgcca ctggcatcca aaccctggaa gattataagt acagaggatt ttttttctgc
     1921 ataaatgata cgctgaagct cacctatctt ctttgcgagt gattcggttt tggatttgca
     1981 cttatccttg tcaaggtcag aaggtggttt ggtcgagatt ttatttattt ttatcatagc
     2041 ttaaacagac attttacttc gtttcaacaa ataactttgt aatatgggtt tgaacccttc
     2101 attgatgtca gcttctacaa gatctatttt gaattgcata catttcaact ttagctgatg
     2161 aaggtatttc tgcatatttt ctttataaaa tgattttact tcattggact gtagtttaat
     2221 tttttctcct gtttcaagat ctacaaacag atagggtcgg ttttcgtatt caaattctaa
     2281 ttctttactt ttgtcactaa cgtgaaacaa aactacttca tgtttggcat atttgaggtg
     2341 ttgtagagca gagaataatt catcggcctc agaaatattt tcaaacatgt cactaaagat
     2401 tataacaagg gaacgtttat ggatactatc ggctacctgg tgcaatgtcg cagctgctga
     2461 agttgatttt ccgtgtttgc cttcttgcag gagtttttca agataagaca acagcagttg
     2521 ataatgctgg ccggatgatt tgtcggcagt atgtgtacgt atttcgctgt caaaaattgt
     2581 aagaccaaaa gcatcccgtt gtcttttcaa caaattcatc aaagctgctg tggctagaat
     2641 cgaaaaacgc attttatcca aatctccttg tttttccttt gtcggaaaat acatggatga
     2701 agatgcatcc aatacgatat ggcatctaag gtttgtttct tcttcaaatt ttttactaaa
     2761 caatttatct gtacgtgcaa atactttcca gtctatgttt ttggtacttt caccttggtt
     2821 gtatatacga tgttctgcga actcaaccga aaacccatga aatggacttt tgtgcaaccc
     2881 aattataaaa ccttctacca cctgattggc taagagttcg aggttagaaa attcacggat
     2941 ttgttggttg tcgaaaaatt tgttcatatt ccgtagtttg gattgaatga ttatctaacg
     3001 caaaaagaag taaattgtgt acaaaaaaac cctgccgatt tgacagggtt tttctttgga
     3061 agttgtatat gcagttgatt agttcaacaa tgcttcaagt ttttgaacca ttgcctgctt
     3121 ggtagtgaca ccaacctgct tatcaaccac ttcaccattt tttataaaaa ggatagtagg
     3181 aatacttctg atgccatatt tcattgaaac attcgggttg tcatcaacat taagtttacc
     3241 tatggttgct tttccgtcgt aatcttttga aaggtcttca atgatagggg ttatcattcg
     3301 gcaaggtccg caccactctg cccaaaaatc cacaactgaa acaccaccgt taagtgcaga
     3361 gtcctgaaaa ttgtcgtcag taaattcgaa agccatatct tttgtttttt tatttaatga
     3421 atagggtttt acatataact atcaataatt aatgaacata caaaaataat aaaagttccg
     3481 atgattaaaa taatttgacc aaattaacga tttattaata aatttttaag caatcagttc
     3541 ttgaatagca aattaattac tttattattt atcattttta ctgttatctt tctgcgccag
     3601 ttcccgcaat ttattgaaag gtattacagc gattctatct ttcttgtcat agcaggtagt
     3661 cttcagtcca taagttcggt attacggttt ccactttttt ttctggtttt agcaatcata
     3721 ctttattacc tgctcaaccc acaattttac aaaaagccgg gcacgatggt tcacataatt
     3781 tcctggtgta gctatatcat agcattcttt tctctttcat ggggattgaa ctacgcacgt
     3841 acagacattt cagaaaaatt atcatttaat cctatcaatt tgcaagacac tgttttgtca
     3901 agacttctaa cagaagccag tcaggagtta aacactactg ctgagatgat tggtagagac
     3961 acacctagag aacttgaaca ggaatacctt gaagacagta ttcctatgct ggtgagagga
     4021 gagatgcaag agttggggta tcgcgtacct aagagtgtag tattaaaaaa cttcataccg
     4081 gaaggctggc tgttaaggtt tggtaccgcc gggttttatt ttccgtttac tgccgaatgt
     4141 tacatagaca gtggtctgca ccccattcag aaagcctttg tagcagctca tgaatgtgcg
     4201 catgggtacg gtatcggaga cgaaggaacc tgcaatttta tagcttatct ggcttgcatc
     4261 cggtcagata aattattagt taaatattca gcacaatttg catattggag aaccattaag
     4321 gcaatggctg taaaccagca tatattgaaa gaaaaattac tcaatgatat tgcattgggt
     4381 gatcttaaag cagtcataga aaagatgaag aagtatccgg atgtttttcc ggcagccaga
     4441 gatttccttt atgaacatta tctcaaatta caaggtataa aagaagggga ggccaactat
     4501 gaccgtttca ttgaattgtt ggttcaatac aaaaacaagc agtgaatcta ttgcccgaaa
     4561 acaagagtga gatgaaaatt tacaattttt tcttgtggtc gaatgcgata ccatagctaa
     4621 gccctacacc aggatgatat cctatttgcg gattatattt ggctgatgct tcaactacta
     4681 atgtttccaa aaccacaata ccgaacccga caccaaactg tgccggtgcc gtctgaccac
     4741 caacgcggat aatgactctt gatgaaggtg catattctac accaaaccgg aaatcttcgc
     4801 tgtgttcgag tgttttacag aattcgcctc tgattgccag cctgtcattg ggtgtgtaca
     4861 tcgcacctac cgtgaaaata ccaggcagat ggtcattgtc agttattttt tgtcttcccg
     4921 gattagaaac cgagaagaag agtgaaatgc gcctatttaa ctcagaatat aatcctagct
     4981 gaaatccgac tacacctttt ttgccataat caggaatgtt gaaatactgg tactggatgg
     5041 atccacctac cgaaaggttc gaaaataatt ttcggccata cgaagcagat ataagatttt
     5101 cattgtacaa atcaaaccca aacctaccca acgacaaacc aaatgacccg gaatgtgtag
     5161 gtaatgcaat gccggcagaa aatgctgaga gttctttaat accaaaacgt tgatcagcaa
     5221 atatcagagc ctgcatattc ttaatagaag caatagacgc aggattagca gccaagcctc
     5281 gaacatcggc aaacgaagac gatacacctc ctaaagcatt tgagctggca ttgttaagag
     5341 tgccggttac attctgagca gttgccaaag tggcctgcac catcagcaat atcgtagtaa
     5401 taattgagat caaaagtttc attaaccaaa ggtaaaaata ctttccgaca ctattaataa
     5461 tgtcagtcat attttggtat tccgaattcg taagattaat tagtccgtat catttttgaa
     5521 atataattgt atctttgcgt ttcacactcg acacagcaaa ttgagaaaac tattcttggt
     5581 atttgtaatt ggtttggtag gcttcatcat ttatgcgagg cataatcata tagtaccttt
     5641 tgataaaatg cccctcaaaa aagccagaga agaggcagtc ataagcaata tgattgagga
     5701 cgacttttat catttcgaag cagaaatccc gaataccatc cgcttgggcg gcggcaattc
     5761 aataatagta tatgatacta ccgccgggaa agtaatcagc cataatcagt taattaacgt
     5821 aaaaacagac ttcaaccact tctttgaagg ttttgaaaag aataaagcga aaaaagaatt
     5881 agatagcttt aatattgcgc tcgttgattt ttctcctaat gagccatatt tcaatctgtt
     5941 aaagacaaat gtgagccgtg gattttattt aggtatttat agggaactga tagaaaatat
     6001 ccaaagctgg cagaaacggt ataattccgt aattgtattg tcaaagattt ttccaagaga
     6061 agatggttct tctgacttgc ttattctgtt gcattcctat ttaaaaaaga gaaataattc
     6121 tcctgaaaaa caactaaaag ccgaagtggc tttcctgatt ccatgtcgct ctactacaca
     6181 gcatttgctt gattttgtac ttccaaaaga ttttaatggc tcggcggatg taaaacattt
     6241 tttgaagatg tttcattaca gttatccgga tatcaagacg gtcaggaagt actggaagtt
     6301 tgatccaaaa cgttttgaac tcagaaacct aaaatggaat tttgattaat atcgggtatg
     6361 cctaatagag attcattcat caataatata caacaaggat atagttttgc aagtccttca
     6421 atcatgcttg gtgctgctaa ttttgaagga gaaataatta aagaaactca gattagtatc
     6481 cctctaaaaa cacttaaccg tcatgggttg atatccggag ccaccggatc tggaaagacc
     6541 aaaacattac agattcttgc agagcaaatg tccaaatacg gtattccttc attattaatg
     6601 gatataaagg gagacttgag tggtattgca atgccagaat cgactaatac gaagatagct
     6661 gaaagaagtg cgcaggttgg tattccattt gcacctgaag gaaaccatgt cgaattcctt
     6721 accatttcgg atgaagcagg tacaagactt cgggctacgg tgtctgaatt cggtcctgca
     6781 ttattttcca aaattcttga gctgaatgat actcaaagtg gccttgtatc agtaattttt
     6841 aaatattgtg atgattatag gctgcccttg cttgacctca gcgatatgaa agctgtactc
     6901 caacacatca cttcagatgg aaaagaacag tttgcacaag agtttggtgt ggtttctacc
     6961 gtttcggcag gtattatact ccgcaaaatc attgagcttg aacaacaagg ggcgatgaaa
     7021 ttttttggcg aaatatcttt tgaagtttct gacctcgtaa gacaagatga gaatggaaat
     7081 ggcatcattt cgattgtacg actcacggat atacaagaca aacccaagct cttttctacc
     7141 tatatgcttc aaatgttagc cgagatatat tcttcttttc cagaagaagg tgaccttgac
     7201 aaacccaaac ttgcaatatt tattgatgaa gcacatcttg tatttcaaga agcctccaaa
     7261 gtgcttttgt cacagataga aacaatcatt aaactcatac gctctaaagg cgttggcgtc
     7321 tatttcataa cgcaaaaccc tacagatata cctgatgcag tgctaagcca gctgggcctt
     7381 aaagttcagc actcacttag agcatttacc gccaaagaca ggaagaatat taaccttgca
     7441 gcgcagaatt atccgctcac tgaatactat gatgtagcag cattgataac gaatttgggt
     7501 ataggtgaag caatcattac cgcactgaat gaaaaggg
//
LOCUS       DAOQQY010000119         7516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3927_length_7516_cov_6.187642, whole genome shotgun sequence.
ACCESSION   DAOQQY010000119 DAOQQY010000000
VERSION     DAOQQY010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3927_length_7516_cov_6.187642"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLE23_06920"
     CDS             <1..842
                     /locus_tag="PLE23_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HMX83030.1"
                     /translation="VSTIMPYTSTDEAVELSKMGKGSLCSSIITHDNAVAREYTLGAA
                     SHHGRILVLNRDCAKESTGHGSPMPLLVHGGPGRAGGGEEMGGMRGVLHYMQRCAIQG
                     SPSTITEITRVYQPGAEFKDPGVHLFRKYFEDLQVGETVITHKRTVTDSDIVNFANVS
                     WDHFYAHTDATSLDGTIFTQRVAHGYFVLSAAAGLFVDPGKGPVLLNYGLEECRFTKP
                     VYAGMTIGVRLTVKEKVSQEKKNEEDIAKGIVKFMVDVYDETGETVAIATILTMVKKR
                     NQE"
     gene            846..1619
                     /locus_tag="PLE23_06925"
     CDS             846..1619
                     /locus_tag="PLE23_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMX83031.1"
                     /translation="MSLNSTEGGYVNLQTDNGIGTITFFHPSHNSMPGYQLQALANTI
                     TQAGNDDSIKVIILRSAGDKTFCAGASFDELINIKNIDEGRIFFSGFANVINAMRTCP
                     KFIIGRVQGKAVGGGVGIAAAVDYCFATKAASVKLSELAVGIGPFVVGPAVVRKIGIS
                     AMSQLSIDATSFYSADWALNKGLYAGIYETADEMDTAIHALADRLVQSNPEAMKSLKQ
                     ICWEGTENWDTLLANRAEISGTLVLSDFTRNAIEKFKAK"
     gene            1625..3691
                     /locus_tag="PLE23_06930"
     CDS             1625..3691
                     /locus_tag="PLE23_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HMX83032.1"
                     /translation="MASKQIVNKETLEKAFRLMATAKAMTDIYEENFKFVSKYVHATS
                     RGHEAIQIATGLQLLPQDYLSAYYRDDAMLLSIGMRPYDLMLQLMAKRDDPFSGGRSY
                     YSHPSLRDDDKPKIPHQSSATGMQAIPTTGVAMGILYKEKTGLAEQLDINPVVVCSLG
                     DASVTEGEVAEAFQMAALKQLPILYLVQDNEWDISAYSTEIRTMDAARYAQGFPGIEV
                     RSIDGTDFILCYNTIEEVINKIRTERRPFLVHVKVPLLNHHTSGVRKEWYRDDLEEQK
                     LRDPYPKLITTLLKNGFTEQQITAFETKARESVLADYEQSKAAPDPTPDDLFTHAFAP
                     TPVTVESGTRTPEGGEEKVMVDCALFGVQELMEVHPDCLLYGQDVGARLGGVFREAAT
                     LAQKFGSDRVFNTPIQEAFIIGSTVGMSAVGLKPIVEVQFADYIWPGLNQLFTEVSRS
                     CYLSNGKWPVSCIIRVPIGAYGSGGPYHSSSVETVVANIRGVKIAYPSNGADLKGMLK
                     AAYYDPNPVVFFEHKGLYWSKVKGTDAARTIMPADDYILPLGMANIVLEADADKVSNG
                     NTCVIVTYGMGVHWALNAARSFNGRVEIIDLRTIYPLDTDTVFNAVRKHGKCLVVTEE
                     SINNSFAQSLAGRIQEACFTSLDAPVMTIGSENLPAIPLNSTLEETFIPSISKVEAAI
                     QRLLDY"
     gene            4612..5883
                     /locus_tag="PLE23_06935"
     CDS             4612..5883
                     /locus_tag="PLE23_06935"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMX83033.1"
                     /translation="MQVLIIYFIYLYYENFTNRTEYINKLLTYKDKDLIKVVSGLRRS
                     GKSTLLEIYRELLLKQGIGKRQIQYYNFELPVNFVNKNWDDIYFDIKKKLQTDKPNYI
                     FLDEVQNILQFEKLVDGLFASENTDVYITGSNAFLLSGELATLLSGRYIEISILPFSF
                     IEYLTARKIDTSIKYLNYENLFFDYIKETSLPKGIELRESGFDEIYEYLEAIYNTIVE
                     KGITQRQKINDKRAFGNIVKFVASNIGNALSPSNISKTLKQDGQSIHHATVEKYLEYL
                     VASFVFYKVNRFDLKGKKQLATQEKYYLVDAGLLNILTGKERTTDRGHILENVVYLEL
                     LRRGNKIWIGTSRNTEVDFVCKTKTGDIEYYQVAWQLTNEKTIEREFGALEKISDNYP
                     KFLLTTDGFTQDRGGIKHLNVFNWLLETNEK"
     gene            6377..6982
                     /locus_tag="PLE23_06940"
     CDS             6377..6982
                     /locus_tag="PLE23_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008992701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMX83034.1"
                     /translation="MQEFTDRQIEIMEAATNRISKFGIQNLTIKTLAEDIGLSEPALY
                     RHFKSKNQILWSLLEYFKTEMKNRIQSIPFKPSTSEADKLRAIFNSQLQTFVNKPAIV
                     SVIFAESIFHYEENLSNKVSEIMDMMQNYVKTNIKQGQESGQYNKLMGASTLTTILIG
                     GIRMTVLKWKLSGHKSDLIKDGKTVLDGILKMIENKNTIKY"
     gene            7045..7428
                     /locus_tag="PLE23_06945"
     CDS             7045..7428
                     /locus_tag="PLE23_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83035.1"
                     /translation="MKKVISIFILFTFLSVNTAFWEVLKLPLLIHHYIEHSQEDNNNS
                     IINFLAKHYGKDINHHHNDHHHDHDKLPFKTINVHSVQVVYFQPLFIKFSRQIAGKEL
                     KTPILQQQNYSNAYLNSIWQPPKVS"
BASE COUNT         2395 a         1626 c         1516 g         1979 t
ORIGIN      
        1 cggtatctac cattatgcct tacacatcta ccgatgaggc agtagagctt tcaaaaatgg
       61 gcaaaggctc gctgtgcagt tctattatta cacacgacaa cgctgtagcc cgcgaatata
      121 cacttggtgc agccagccac cacggcagga tactggtact caacagggat tgtgccaagg
      181 aaagtaccgg ccacggcagc cctatgccat tgctagtaca cggcggtcca ggtcgtgccg
      241 gcggaggcga agagatgggt ggcatgcgtg gtgtattgca ctatatgcag cgttgtgcca
      301 tacaaggttc accatcaact attacagaaa tcaccagggt ctatcagccg ggtgcagaat
      361 tcaaagaccc gggtgtacat ctgttcagaa aatacttcga agacctccag gtaggcgaaa
      421 cggtcatcac acacaaacgc accgttaccg atagcgatat tgtcaatttt gccaatgtaa
      481 gctgggacca cttctatgca catacagacg ctacatcgct tgatggtacc atatttaccc
      541 aaagggtagc tcacggctat tttgtactct cagctgcagc tgggttattt gttgacccgg
      601 gcaaaggacc cgtattgctc aactacggcc tcgaagaatg ccgcttcacc aaaccggtat
      661 atgccggtat gaccattggg gtcaggctca cagttaaaga aaaagtaagc caagaaaaga
      721 aaaatgaaga agacatcgcc aaaggaattg taaaattcat ggtagatgtt tatgatgaaa
      781 caggtgaaac ggtagccatt gccaccattc tcactatggt taaaaaacga aatcaggaat
      841 aaactatgtc actcaactca acagaaggcg gatacgtcaa tctgcagaca gataacggta
      901 tcggcaccat caccttcttt cacccttcac acaatagtat gcccggctac cagttgcagg
      961 ctcttgccaa taccatcact caggctggca atgatgatag tatcaaggtg attatactac
     1021 gtagcgcagg cgataaaact ttctgcgcag gggcaagttt tgacgaactc atcaacatca
     1081 aaaatattga cgaaggccga attttcttct ccggcttcgc taacgttatc aatgccatgc
     1141 gtacctgtcc caagtttatc ataggtcgtg tacagggcaa ggcagttggc ggcggggtag
     1201 gtattgcagc agccgtagat tattgcttcg ccaccaaagc tgcctccgtc aaactcagtg
     1261 aactcgctgt tggcatcggc ccctttgttg ttggcccggc agtagtgcga aagattggca
     1321 tctctgccat gtcacagtta tccattgatg ccacttcatt ctatagtgca gactgggcac
     1381 tgaacaaagg cctctatgcc ggtatatacg aaacggcaga tgaaatggac acagccattc
     1441 atgcactcgc cgaccgcctg gtccagtcca acccggaggc tatgaagtcg ctcaaacaaa
     1501 tatgctggga aggtaccgaa aactgggaca cactgctcgc caacagagca gaaataagtg
     1561 gcacactcgt attatccgat ttcacccgaa acgctataga gaaatttaaa gctaaataac
     1621 aatcatggca tcaaaacaga tagtgaacaa agaaaccctc gaaaaagcat tcaggctgat
     1681 ggctactgcc aaagccatga cagatattta tgaagaaaat ttcaaattcg tctctaaata
     1741 cgtacacgcc acctctcgtg gacacgaagc catacagata gccacaggcc tccagttgct
     1801 tccgcaagac tacctctctg cttattaccg cgatgatgct atgttgctgt ctataggtat
     1861 gcgtccctat gacctcatgc tccaactcat ggccaaacgc gacgacccat tctccggtgg
     1921 tcgttcttac tacagccacc caagcctacg cgatgatgat aaaccaaaga taccgcacca
     1981 gtcttctgct acgggcatgc aggctattcc caccacgggt gtagccatgg gcatactgta
     2041 caaggagaaa accggtctcg cagaacaact ggatatcaat cctgtagtag tgtgctccct
     2101 gggcgatgct tccgtcacag aaggtgaagt agccgaagca ttccagatgg cagctctcaa
     2161 acaactgccc atactttacc tcgtacaaga caacgaatgg gacatctcag cctattctac
     2221 cgaaatccgt accatggatg ctgcacgcta tgcacagggc ttcccgggta tagaggtgcg
     2281 tagtattgac ggcaccgatt ttatactctg ctacaataca atagaagaag tcatcaacaa
     2341 aatccgtacc gagcgtcgac cttttctcgt acatgtcaaa gtgcccctgc tcaaccacca
     2401 taccagtggc gtacgcaagg aatggtaccg cgatgacctc gaagaacaaa aactgcgcga
     2461 cccgtaccca aaactgataa ctacactact aaaaaacggt tttaccgaac agcagataac
     2521 cgcattcgaa actaaagcaa gagaaagcgt actggccgac tacgaacaat caaaagcagc
     2581 acccgaccca acaccggatg acctctttac gcatgctttt gcccctacac cggtcacagt
     2641 agagtcgggt acacgcacac cagagggcgg cgaagagaaa gtaatggttg actgcgccct
     2701 gttcggtgta caggagctga tggaagtcca ccccgattgt ttgctttatg gccaggatgt
     2761 aggtgccaga ctcggaggcg tattccgtga ggcagctaca cttgcacaaa aattcggcag
     2821 cgaccgggtt ttcaatacac cgatacagga agcctttatc atcggcagca cagtcggtat
     2881 gtcggctgtc ggcctcaagc ccattgtcga agtccagttt gccgattaca tctggccagg
     2941 tcttaaccaa ttgtttaccg aagtaagccg ctcttgctac cttagcaacg gcaagtggcc
     3001 tgtcagttgc atcatccgtg ttcccatcgg tgcttatggc agtggcggtc cttatcattc
     3061 atcaagtgta gagacggtag tagccaatat tcggggcgtc aagatcgcct acccgagtaa
     3121 cggagccgac ctcaaaggta tgctcaaggc agcctattac gaccccaatc ctgttgtgtt
     3181 ttttgagcac aaaggccttt actggtctaa ggtcaaaggt accgatgctg cacgcaccat
     3241 catgcctgcc gatgactaca ttctgcccct tggtatggca aatatcgtgc tcgaagcaga
     3301 tgcagacaaa gtttcaaatg ggaatacctg cgtaatcgta acgtatggca tgggcgttca
     3361 ttgggcactc aatgcagctc gtagcttcaa cggtcgggta gagataatcg acctgcgcac
     3421 catttatcca ctcgacaccg atacggtatt caacgctgtc cgtaagcacg gcaaatgcct
     3481 agtcgttacc gaagaatcca tcaacaattc ctttgcccaa agccttgcgg gacgcattca
     3541 ggaggcgtgt ttcacctcac tcgatgcacc tgtcatgacc atcggctcag agaatctacc
     3601 ggccataccg ctcaatagta cactcgaaga gaccttcata ccatccatca gcaaagtaga
     3661 agccgccata cagcgtttgt tggattattg atcagccggg ttggtttatt atttgggcgc
     3721 ctccctgccg ggtcgggctg tacggcttac gttgtgccgc ctgccatccc tggcgcaatg
     3781 tagttataca atcagtcatt cattccggat aaagcattat caattttaac gaaaatgatg
     3841 agtacataag atccataaaa aaggggtaga agcgtagtga cctagggttg ttgagctttt
     3901 gggttaaagt agattatctc gatggcaaac ctacttgaca cagcagtaaa catacattta
     3961 ccataaactt acgattaatt tacactataa tcgtaatatt acggtaaata atccttttat
     4021 caacacaatc accacgatat gaatgtatta caccttatgt ttgactgata atcccagata
     4081 atccattagg ttatgagatc taatatgatc ggatttctct aatccaatga agctgggcat
     4141 tctcgaagat gcacatccaa tttccttatt gaaaaagttg taagatttcc ttacagggaa
     4201 accaaaagtt ttacctcgtt attatactca gtaattatct gataaacagt tttttatgtc
     4261 gttatttttc taatggcatt caatagagat tttacactat tgaagttttt gggataatat
     4321 tgtgtcatga aaaaattaat tatttacaaa ataatatccg gcgatgccag aactattagt
     4381 ccataacagc atcaagacca ccaccattta tatccacatc accgaccgta tgaagtccaa
     4441 cacccatagc cctccggatg atttgcatct atgaaaaaaa aggaatacct ttggtgtatt
     4501 tacgccattg tggcgtaaat aaaaacgttt tatccaatgg tacagtgccg tgccaatatc
     4561 agactgacat ataattacaa gactatttaa tattatcaaa acttacacat tttgcaagtt
     4621 ttgataattt attttatata tttgtactat gagaatttta ccaatcgaac ggagtacatc
     4681 aacaaactac ttacctacaa agacaaagac ctcataaagg tggtgagtgg tttgcgtagg
     4741 tcgggcaaaa gcactttgct ggaaatttac cgtgaactct tgcttaaaca aggtattggc
     4801 aaacggcaaa ttcagtatta taactttgaa ttgcccgtaa attttgtaaa caaaaattgg
     4861 gacgacattt attttgacat caagaaaaaa ctacaaacgg ataaacctaa ctatattttt
     4921 ttagacgaag tacagaacat tctacaattt gaaaaattgg tggacgggct atttgcttca
     4981 gaaaacacag atgtttacat cacaggctca aacgcatttt tgttgagtgg cgaattagca
     5041 actttattga gcggtcgata tatcgaaatt tctatcttgc ccttttcttt catagaatat
     5101 ttaaccgcac gaaaaattga cacaagtata aaatacctca attatgaaaa tttgtttttt
     5161 gattacatca aggaaacttc gttgcccaaa ggtatcgaat tacgagaaag tggttttgac
     5221 gaaatttacg aatacttgga agcaatttat aacaccattg tagaaaaagg catcacgcag
     5281 cgccaaaaaa taaatgacaa acgtgccttt ggaaacatcg taaaatttgt agcctctaac
     5341 attggtaatg cactttcgcc aagcaatatt tctaaaacat taaaacaaga cggacaaagc
     5401 attcatcacg caaccgttga aaaatatttg gaatatttgg tagcgagttt tgtgttttat
     5461 aaagtaaacc gttttgacct taaaggcaaa aaacagttgg ctacacaaga aaaatattat
     5521 ttggtggatg caggtttgct caacattctc acaggaaaag aacgcaccac cgacagagga
     5581 catattttag aaaatgttgt ttaccttgag ttgcttcgca gaggcaacaa aatctggata
     5641 ggaacaagtc gtaatactga agtggatttt gtatgcaaaa ccaaaacagg tgacattgaa
     5701 tattaccaag tagcgtggca actgacaaat gaaaaaacta tagaacgaga atttggagca
     5761 ttagaaaaga taagtgacaa ctaccctaag tttttattga ccacagacgg atttacacaa
     5821 gaccgaggtg gcataaaaca tttgaacgtt tttaactggt tgcttgaaac aaatgagaaa
     5881 taatcaccaa tggctataac aagcagtttg aacgaaggcg agtaaagtgc ttcgatttga
     5941 cattgccgta agaaaaagta ttaacttcgt atcaacactt gtcggtaaaa ttcgtcctgc
     6001 gtcaagccgt caaaaccgtt ttaagccatt ccccattttt ctgcaagaaa ataaattttc
     6061 atgctaatgg gagagccatc cggcagacag attcacatac atttgtagtg gctcattgta
     6121 agaccctttt aagcattggt gcaacaaagc tcgtgcgtca ttctggttgg gaatgtaaaa
     6181 agcccgaaca agtaatttgg gcttggtgat aaattcagtg gtggagtccg tgtagcaatc
     6241 aggactatcc cgttcagaat aatttttcct cctatcaaca aatcaaattc aatttatttt
     6301 taaaataatt cttgcagaat gaaaaatttt atttattttt gcgagtgaat gctaactaac
     6361 ttgatttaat ttgaagatgc aagaatttac agacagacaa atagagataa tggaagcggc
     6421 aaccaacaga atttcgaaat tcggaattca gaatttgact ataaagacac ttgctgaaga
     6481 cattggactt tcagaacctg cattataccg tcatttcaaa agtaaaaacc aaattctatg
     6541 gagcttattg gagtatttca aaacagaaat gaaaaaccgt attcaatcta tcccgtttaa
     6601 accatcaaca agcgaagctg ataaattaag agctattttc aattctcaat tacaaacttt
     6661 cgttaataaa cctgcaattg tcagtgttat ttttgccgag agtatttttc attatgaaga
     6721 aaatctaagt aataaagttt ctgaaataat ggatatgatg cagaactatg taaagaccaa
     6781 tattaagcaa gggcaagaaa gcggacaata taacaaattg atgggagcat ctacgcttac
     6841 gacaatttta attggcggta tcagaatgac tgttttaaaa tggaaattgt cggggcataa
     6901 atcagactta ataaaagacg gaaaaactgt tttagacgga attttaaaaa tgattgaaaa
     6961 taaaaataca atcaaatact aacatcaacc aaaaaactga ttaatgtcat aatttaattg
     7021 attattatta ctgaactttg cagtatgaaa aaggtaattt ctatattcat tctttttact
     7081 tttttaagtg tcaatacggc attttgggag gttttgaagt tgcctttact tatccaccat
     7141 tatattgagc attctcaaga agataacaac aattcgatta taaatttttt ggcaaaacat
     7201 tatggaaagg acattaatca ccaccataat gatcatcatc acgaccacga taaacttccg
     7261 tttaagacaa ttaatgtaca ttctgtacaa gtagtatatt ttcagccttt gtttatcaag
     7321 ttttcaagac aaattgctgg gaaagaattg aagacaccta tacttcaaca acaaaattat
     7381 tctaacgctt acctcaacag catttggcaa ccgccaaaag tttcttagtt ttttattaga
     7441 caatagacaa tagacaatag gcaataggca ataggcaata ggcaatagac aataggcaat
     7501 aggcaatagg caatag
//
LOCUS       DAOQQY010000120         7500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3945_length_7500_cov_5.622028, whole genome shotgun sequence.
ACCESSION   DAOQQY010000120 DAOQQY010000000
VERSION     DAOQQY010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7500
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3945_length_7500_cov_5.622028"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1324
                     /locus_tag="PLE23_06950"
     CDS             296..1324
                     /locus_tag="PLE23_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMX83036.1"
                     /translation="MKAIRLTALNKPLEMQEIPIPIIGVNDILVKVKAAGICHSDAHY
                     RAGLSPVSYMPLTLGHEVAGMVEATGSGVANIKQGDRVCLHYLISCGNCHFCNSGQEQ
                     FCPTGKMIGHHTDGGYAEYIAVPARNAVLLPDTISFEQGATLMCASATSFHSLLKGRI
                     KHGDNVAIFGVGGLGQSAVQLAFALGANKVIAVDINEEKLRLAAEYGAIQVNPQKTDP
                     VAEIRKHTENVGVDLAVEMIGLPLTQKQALQSAGPLGRVVLVGLSDKPLSVDTYHEVL
                     GKEVELIGSNDHHLNELPQLIHFAAEKILDVSKVVSRTIPLEANAINDALDALERFDS
                     GVRTVIVP"
     gene            1352..1786
                     /locus_tag="PLE23_06955"
     CDS             1352..1786
                     /locus_tag="PLE23_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMX83037.1"
                     /translation="MIYNEEYFIARCELLGVKATEQGNPSVGAVIVKNGEIVAEAAEA
                     GKSKNDITCHAEIEALRLAVKKLQSNDLSECVMYSTHEPCIMCSYAIRYYRVKKLVYL
                     HDVDYLGGITSQMPLLISDIVPPHWGRPPEIVRYRKNLNKMV"
     gene            1997..2524
                     /locus_tag="PLE23_06960"
     CDS             1997..2524
                     /locus_tag="PLE23_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83038.1"
                     /translation="MKNLILSIGVLILTSMTASAGSCQSNIEQDTLNPLSFPFEVAKY
                     YEEYTKGFQEGKQLNDQDVIQFGKHFNFDNVENFLTFYLHSIDTLKNHFSQNPTKITS
                     NNPNDDLSPGLPCYEQWKADVMNYLSGALLVAGEMGIFTGGNFVAVGATLVFNSSVCL
                     IQAQNSYNHCIKGTY"
     gene            2558..2896
                     /locus_tag="PLE23_06965"
     CDS             2558..2896
                     /locus_tag="PLE23_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83039.1"
                     /translation="MKSKILLIGFLLGFMACKSDGDKLKPEELYAKWNHSKTILAYKA
                     AINEMAMFNVNNFTKAGKNYDFDEIVELSSKLSDNDAKSTAGLSDKELAAIGYAKCVK
                     NVIDTKLAAR"
     gene            3032..3268
                     /locus_tag="PLE23_06970"
     CDS             3032..3268
                     /locus_tag="PLE23_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83040.1"
                     /translation="MIGIFVLSVTLGFAKSCLDGFSDSVNLTQLTLNYDMEVLCDGTI
                     LIANSNCINEANKKYEKALDDALTTLCVCDPAWC"
     gene            complement(3350..3862)
                     /locus_tag="PLE23_06975"
     CDS             complement(3350..3862)
                     /locus_tag="PLE23_06975"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMX83041.1"
                     /translation="MDRPIFQYFEEDHRRLDQLLANACAVDGKVDTECYHRFRTGLLR
                     HIKMEEKILFPAARKAANGKFIPLADKLRLDHGAITALLVFPPDKDVIKVLNYILVQH
                     NQLEEQAEGMYDICENLTGEYTEDVLAQLRQTPEVPVNPFNSSENAFEATKRALSRAG
                     YDYNEILSSI"
     gene            complement(3904..4662)
                     /locus_tag="PLE23_06980"
     CDS             complement(3904..4662)
                     /locus_tag="PLE23_06980"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMX83042.1"
                     /translation="MVTLEKNENGELDVRVLVPIQRHELLIRLFKELPAHDNFIFIND
                     HDPIPLYYEFRSIYGDVVGWEYLNRGGREWKVKVTKTEESLGREFTDISTLLDLRKAD
                     KPEWKQIVFHRYGMMEEGTTMELVSKEDPTEIHGIFIHKFEGRHKWVYKKKEDGEYVI
                     HLTKQDNSGLGDDGFSVVNEMDIRPYPPTERHEMFYKAFADIKSGEAFEFINDHDPKP
                     LYYQMEAESKEPFRWEYLEKGPDVWKVRVTKVNE"
     gene            complement(4799..5128)
                     /locus_tag="PLE23_06985"
     CDS             complement(4799..5128)
                     /locus_tag="PLE23_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HMX83043.1"
                     /translation="MANIVTTNDNEKCEKALTGLYNVIDPEIGLNVVDLGLIYRIDFV
                     DDAGRIDLIMTLTTQFCPMGEAITGGVMNSVTDAFPGWDVNVQLTFDPPWSFERISPD
                     GKQFLGH"
     gene            complement(5249..6961)
                     /gene="ric"
                     /locus_tag="PLE23_06990"
     CDS             complement(5249..6961)
                     /gene="ric"
                     /locus_tag="PLE23_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HMX83044.1"
                     /translation="METAQETIINVQELEPKLRHATIFQTFDELKEGESLIIHNNHDP
                     QPVFYQLMDMRGNIFAWDYLQKGPEWWDIRVTKLVGNISGRIDEFILNVPGIKPESKH
                     AAIFQVFKGTDAGESFIIHNDHDPKPVFYQLQNEFGDSFTWEYIHEGPQWWNVQITIK
                     QVAPERDENGEIVINVPSIEPRLKHQTIFNTFDNLKPGEAFIIHNNHDPKPVFYQLNQ
                     MHGDVFTWEYLQEGPQWWDIRVTRKPLETNISNENHPSPKEITIDVPSLEPKLKHSTI
                     FDTFDRLQPGESMIIHNDHDPKPVYYQLKSERGDIFTWEYLQQGPQWWDIRVTKNATV
                     TQETIGEIVAGDIRKAEVFKKFGIDFCCNGKKTIQEACAAKDIDPAIVEAELRKPVEG
                     TATANVNYTEWEPDFLADYIVNTHHRYVKKYLPEIRGYSIKVAQVHSNQHPELKEIKD
                     LFENINEELTEHMHDEEGKLFPIIRQIVHAKNQNTRIVNETVEQYKTMTADAEKEHDS
                     VGRAMENIRKLSQNFEIPPDACTSYKLLFRMLEEFENDLFVHIHLENNILFPKTEEIL
                     TTFG"
     gene            complement(7140..>7500)
                     /locus_tag="PLE23_06995"
     CDS             complement(7140..>7500)
                     /locus_tag="PLE23_06995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX83045.1"
                     /translation="IVPGMFIQAGGLFLITGQLFTPLTSGLLGSALLGIGTAMVYPTL
                     MAVVSDSVHPARRASSLGVYRFWRDMGYAVGALFAGITANMLGFDWTIRLTAIITALS
                     GIYVWVHLKENKEQTTA"
BASE COUNT         2100 a         1449 c         1575 g         2376 t
ORIGIN      
        1 ttaaaaatac ggcagctgaa gtaggaggtg ccgaacgttt tatccaagaa agacacatta
       61 gtaattgcta caaagctgat cctgcttacg gaaaaggtgt ggccgatgcg cttggtatcg
      121 atatccattc gcttgatttg aattaatgtg taaatattaa gcatacttca aaccggtttt
      181 tgcctaatct cttgcgcaga aaccggtttt tttatttcat ttgaaatccc tacagagcct
      241 tcgaatttat tcggatacat tttaccacct tattgtttat tctatgagac agaaaatgaa
      301 agcaatcaga ctgacagctt tgaataaacc actggaaatg caggaaatac caataccgat
      361 aatcggtgtc aacgatattc tcgtgaaagt gaaagctgcg ggtatctgtc attctgatgc
      421 tcattaccga gctggtttat cgcctgtaag ttatatgcct cttacacttg ggcacgaggt
      481 ggcaggtatg gttgaagcga ctgggagcgg agtggccaac ataaagcaag gtgaccgtgt
      541 ttgtctgcat tacctcataa gctgtggcaa ttgtcatttt tgcaactccg ggcaagagca
      601 gttttgcccg actggtaaaa tgatcggtca ccacaccgat ggtggatatg ccgaatacat
      661 agccgtacct gccagaaatg ctgttctttt gcccgatacc atcagctttg agcaaggtgc
      721 aacactcatg tgtgcatcag ctacatcttt tcattctttg ctcaagggta ggataaagca
      781 cggtgacaat gtggcaatat ttggtgtagg tggattgggc cagtcggccg tacagcttgc
      841 atttgcgcta ggcgccaata aggtaatcgc agtcgatatc aatgaagaaa aactcagact
      901 tgcagccgaa tacggagcaa tacaagtgaa tccgcaaaaa acagatcccg tagctgaaat
      961 ccgtaagcat accgaaaatg ttggtgtgga cttagctgta gaaatgattg gtttgccctt
     1021 aactcaaaag caggctttgc agtccgccgg tccgttgggg cgtgttgtgc tggttggctt
     1081 gtccgacaaa ccattgtccg ttgatactta tcatgaggta ctcggaaagg aagttgaatt
     1141 aataggctca aatgatcatc acctgaacga actccctcaa ctcatccatt ttgcagccga
     1201 aaaaattctg gatgtatcca aggtagtaag ccgaactatt ccgcttgaag caaatgccat
     1261 taatgatgct cttgatgcac tcgaacgctt tgattcaggt gtacgtacag tgattgttcc
     1321 atgaagcaat ttaaatataa tagagaaaaa catgatttat aatgaagaat attttatcgc
     1381 acgatgcgag ttgctcggcg taaaagcgac agaacaaggg aatccatctg taggtgcagt
     1441 tattgttaag aatggagaaa tcgtagcaga agctgcagaa gccggcaaaa gcaaaaatga
     1501 tatcacctgc catgccgaaa ttgaagcatt gaggcttgcc gtcaaaaaat tgcaaagtaa
     1561 tgacctgtcg gaatgtgtca tgtactcaac acatgagcct tgtattatgt gttcgtatgc
     1621 tatacgttat tatcgggtga aaaagctcgt ttacctgcat gatgttgatt atcttggcgg
     1681 tattacatcg caaatgcctt tacttatatc cgacattgtg cctccgcact ggggtagacc
     1741 acctgagata gtacgatatc ggaagaactt aaataagatg gtttgaaaat tttattttaa
     1801 gtttatttta ttctttaata ttttttgttg tttgatgaaa ctattgaagc cctgaaaatt
     1861 gttattcaat agttagttat actttaggct gccggtatgg ttaaagctta gagataaaca
     1921 aaatattcgg ttcaaaatgt taaataaatg ttaaagttta ttattattat tattattgta
     1981 taaatttttt aatagtatga aaaacttaat tttatcaatt ggcgttttaa ttttaacatc
     2041 gatgaccgca agtgccgggt catgtcagtc aaacattgaa caggatactt taaacccact
     2101 tagtttccca tttgaggtag caaagtatta cgaagaatat accaaaggtt ttcaagaggg
     2161 taagcaactg aatgatcagg atgttatcca atttggcaag catttcaatt ttgataatgt
     2221 tgaaaatttc ttaacatttt atctgcactc aattgatact ttaaaaaatc atttttcaca
     2281 aaacccgaca aaaatcactt cgaataaccc caatgacgat ttgtctccgg gattaccatg
     2341 ttatgaacaa tggaaagcag atgtcatgaa ttatctttca ggtgcgcttt tggtggcagg
     2401 tgaaatgggg atttttacag gtggaaattt tgttgcagta ggagcaacat tggtttttaa
     2461 ttcctcagta tgtctaattc aagcacaaaa ttcatataat cattgtataa aaggtactta
     2521 ctaaataatt tttataatca attaaatgaa tattattatg aaatcaaaga tcttgctcat
     2581 tggatttcta ttaggtttta tggcatgtaa atcagatggt gataaattga aacctgagga
     2641 actgtatgcc aaatggaatc atagtaaaac catcttggca tacaaggcgg ctatcaatga
     2701 aatggcaatg tttaatgtta ataattttac taaggccggt aaaaattatg attttgatga
     2761 gatagtagag ctgtcatcaa aattgtcgga taatgatgca aaaagcactg caggtttgtc
     2821 ggacaaagaa ttggccgcca ttggttatgc aaagtgtgta aagaatgtta tagatacaaa
     2881 acttgctgcc aggtaggcca atcctgaaat cttgacccac agggatgaat ttgtaaaatt
     2941 tttagcaggc ttagaaggcg taacaggtga tgatgtttta agaataatgg caagtaagaa
     3001 gtaaatccgg aattctgaaa agccaattgt attgatagga atattcgtat tgagtgtgac
     3061 acttgggttt gctaaatcat gcttggatgg gttcagtgac tcagttaatc ttacccagct
     3121 aacacttaac tatgatatgg aagtcttatg cgatggtaca attttaattg ccaactcgaa
     3181 ttgtataaat gaagctaata aaaagtatga aaaagcttta gatgatgctt tgacaacatt
     3241 gtgtgtttgt gaccctgcat ggtgttagtg tcctgcaagt accggatgcc aaagccggat
     3301 agaatatttc aataaaggag aaaagggtta ttcttatatt atctattact caaatggagc
     3361 taaggatttc attatagtcg tacccggccc ttgacaaggc tcgtttggtg gcttcaaatg
     3421 cattttccga agaattgaaa gggttgaccg gtacttcggg tgtctggcgt agctgtgcaa
     3481 gaacgtcttc tgtatattcc ccggtgaggt tttcacaaat gtcgtacata ccttctgctt
     3541 gttcttcgag ctgattgtgt tgcactagta tataattcaa cactttgatt acgtccttgt
     3601 caggaggaaa caccaaaaga gccgtaatgg ctccatgatc tagtctgagt ttgtccgcta
     3661 acggaataaa ttttccgtta gcggctttcc ttgctgccgg aaacagtatt ttctcttcca
     3721 tctttatatg acgcagcaat ccggtcctga accggtgata gcattcggtg tcaaccttgc
     3781 catccacagc acaggcattt gccaataatt ggtctaaccg gcgatgatct tcttcaaaat
     3841 actgaaatat aggcctgtcc attttctgaa aaccaatcct ggctttataa gactatgggt
     3901 tatttattca ttcacttttg ttacccttac cttccataca tcaggacctt tttcgaggta
     3961 ctcccatctg aacggttctt tgctctcagc ctccatctgg tagtagagcg gtttcggatc
     4021 gtgatcgttg ataaattcga atgcttctcc cgacttgata tcagcaaatg ccttgtaaaa
     4081 catctcgtga cgctccgtag gcgggtaggg gcgtatgtcc atttcattca ctaccgaaaa
     4141 accatcgtct ccaagtccgc tgttgtcctg cttggtcagg tgaatgacgt attcgccatc
     4201 ttcttttttc ttgtacaccc acttgtgtct tccttcaaat ttgtggatga aaatgccatg
     4261 aatctctgtt gggtcttctt ttgatacgag ctccatggta gtgccttctt ccatcatacc
     4321 atagcggtgg aaaacaatct gcttccattc cggtttgtcg gcttttctta ggtcgagtag
     4381 ggtagatatg tctgtaaact ctcgccccaa agattcttcg gttttggtta cttttacttt
     4441 ccattcgcgc ccgccgcggt tgaggtattc ccagcctaca acatcgccgt agatggagcg
     4501 gaattcgtaa taaagtggaa tggggtcgtg gtcattgatg aatataaaat tgtcatgtgc
     4561 aggcaattct ttgaataatc ttatcagaag ttcatgcctt tgtatcggta ccagcacccg
     4621 tacatcgagt tcgccgtttt cgtttttttc aagagttacc atttgagaag tatttatttt
     4681 ttttatatta aaattttttt gaggaaaatg tgtggttcgt ttggcataga aaattccctt
     4741 tgcataaaat atctaaaggg cataattcat attgtacatc caacaaaatc ttgctctgtt
     4801 aatgacctag aaattgtttc ccgtcaggtg aaatgcgttc aaaactccat ggcggatcaa
     4861 atgtgagttg tacgtttacg tcccagcccg ggaaagcatc cgtcaccgag ttcattacac
     4921 cacccgtgat ggcttcaccc attgggcaga actgagtggt gagtgtcata atcaggtcaa
     4981 tacgccctgc atcatcaaca aaatcaatcc tgtagataag acccaggtca acaacattca
     5041 gcccaatctc cggatcaata acattgtaaa gtccggtgag tgctttttcg catttttcgt
     5101 tatcgttggt tgtaacaata ttcgccatat aggtgtccgg aacagtaccg gcattgttgt
     5161 tttactttca ttgtaaaaag agcggccacg aaatattcca gtgtatccaa aattatctca
     5221 tggcgctact cacattagaa cataaagatt atccaaaggt tgttaaaatt tcttctgttt
     5281 tcggaaagag aatattgttt tcgaggtgta tgtgtacgaa taagtcattc tcaaattctt
     5341 ccaacatcct gaacaaaagt ttgtaacttg tacaggcatc gggaggaatt tcgaaattct
     5401 ggctcagttt ccttatgttt tccattgcac ggcctacact gtcatgttct ttttcggcat
     5461 cggctgtcat tgttttgtat tgttcgactg tttcgtttac gattcttgtg ttttgatttt
     5521 ttgcatgtac tatctgtctg atgattggga ataattttcc ttcttcgtca tgcatgtgct
     5581 cggtgagttc ttcattaata ttttcaaaca gatctttaat ctccttcagt tcgggatgtt
     5641 ggttgctgtg tacctgagct actttaatgg aatatccgcg tatttcaggt agatacttct
     5701 ttacatatct gtgatgtgta ttgacgatgt agtctgcaag aaagtcgggt tcccattcgg
     5761 tataattgac atttgcagta gcagtgccct ctacaggttt gcggagttct gcttctacga
     5821 ttgccggatc aatatctttg gccgcacatg cttcttgtat ggttttcttg ccgttgcagc
     5881 aaaagtcgat gccaaatttt ttgaatactt cggcttttcg gatgtcacca gccacgattt
     5941 cgcctatggt ttcctgagtg acggtagcat ttttagttac acggatgtcc caccattgcg
     6001 gcccctgctg cagatattcc catgtaaaaa tatctccgcg ttcacttttc agctgataat
     6061 aaaccggctt ggggtcgtga tcattgtgga taatcatact ttcgcccggt tgtagtcggt
     6121 caaatgtatc aaaaatagtc gagtgcttca attttggctc aagagaagga acatcaattg
     6181 ttatttcctt aggtgaagga tggttttcgt tagaaatatt ggtttccaat ggttttctgg
     6241 tcacacggat gtcccaccac tgtggaccct cttgcagata ttcccaggtg aacacatctc
     6301 catgcatttg gtttagctgg taaaataccg gttttgggtc gtggttgttg tgtataatga
     6361 atgcttcgcc aggctttaaa ttgtcaaaag tgttgaagat ggtctgatgc tttagccttg
     6421 gttcgataga tggcacgtta atgacgattt cgccattttc gtccctttca ggagctacct
     6481 gcttgatagt tatctgtaca ttccaccatt gcggcccttc atggatgtat tcccatgtaa
     6541 aactgtcacc gaattcgttc tgtaactgat aaaataccgg ttttgggtca tggtcgttgt
     6601 gaataatgaa cgattcacct gcatccgtac ctttaaatac ctggaatata gcagcatgct
     6661 ttgattctgg ctttataccc ggaacattca gaataaattc atctattcta ccagatatat
     6721 tgcctaccaa cttcgtcaca cgtatatccc accattcagg acctttctga agataatccc
     6781 aagcaaaaat attgccgcgc atatccataa gctggtaaaa gacaggttgc gggtcatgat
     6841 tgttatgtat gataaggctt tctccttcct tcaattcgtc aaaagtctgg aatattgtag
     6901 catgtctcaa tttgggttct agttcctgta cgttgatgat ggtctcttgc gcggtttcca
     6961 tatatttatt tatgatgata aatgagattt catcacaaag ataggcattt attttaataa
     7021 aagtagaaaa atacttttaa taaaaaagta attttacaat tctgttacaa taacgccata
     7081 ccaagtcatt tttcaatttt acgatattcg actaataacg catgaactat actaatgtac
     7141 taagcggttg tctgctcttt gttttctttt aaatgaaccc atacatatat accagacaga
     7201 gcggtgatga tagcagtgag cctgatggtc cagtcaaagc cgagcatatt ggcggtgata
     7261 ccagcaaaga gtgcgcctac cgcataaccc atatcgcgcc aaaatctgta aacaccaaga
     7321 gatgaagccc tgcgtgccgg atgaacagag tcgcttacta ctgccataag ggtagggtac
     7381 accatggctg ttccgattcc taatagcgcc gagccaagga ggcctgacgt gagaggtgtg
     7441 aataattgtc cggtaatgag gaaaaggcca ccagcttgaa taaacatacc tggtacgatg
//
LOCUS       DAOQQY010000121         7481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_3962_length_7481_cov_5.746566, whole genome shotgun sequence.
ACCESSION   DAOQQY010000121 DAOQQY010000000
VERSION     DAOQQY010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3962_length_7481_cov_5.746566"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..1674)
                     /locus_tag="PLE23_07000"
     CDS             complement(4..1674)
                     /locus_tag="PLE23_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HMX83046.1"
                     /translation="MEITEPIESKNFLQEIIENDIAEGKHDGRVLTRFPPEPNGYLHI
                     GHAKSICINFGLAKQYNGQTNLRFDDTNPVTEDTEYVESIKEDIKWLGFDWANEYYAS
                     DYFDTLYDYATQLIGKGLAYVDDLTAEQIAELKGTPTEPGKNSPHRDRSVEENLDLFR
                     RMKDGEFPEGSKVLRAKIDMASPNMHMRDPLLYRIKYAHHHRTGDKWCIYPMYDFAHG
                     QSDSIETITHSICTLEFIPHRPLYDWCIEKLEIFPSRQYEFARLNMNYTVMSKRKLLQ
                     LVNENHVSGWDDPRMPTLSGMRRRGYPPKSIVEFCDKIGVAKRENIIDIGLLEFCVRE
                     DLNKTATRVMVVMDPVKLVITNYEGTGEHLSAENNPEDQSAGHRNISFGKVIYIERED
                     FMLDPPKGYFRLSPGNKVRLKYAYIIEYENHITDSEGRITEIHVKYYPDSKSGEDTSS
                     IKVKTALHWVPESAINAEIRLYDRLFVVENPNSDKDTDFKEFINKDSMVVNTRAMLEP
                     ILKDADFGKSYQFFRLGYFCLDKDSSSEKLVFNKTVGLKDSWAKILQK"
     gene            complement(1686..3530)
                     /gene="argS"
                     /locus_tag="PLE23_07005"
     CDS             complement(1686..3530)
                     /gene="argS"
                     /locus_tag="PLE23_07005"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMX83047.1"
                     /translation="MNLLHKIQSNTLSILKSMYADKAFVESDIQISPNKTQNKGDYTV
                     LIFPLAKKAGKNPTELGNEIQSGLKNLFPELGFEAIGGFLNIVMNDQFWKDSLEALLA
                     TDFQKMFPENDKTVLVEYASPNTNKPLHLGHIRNILLGWSIYRLLQRLGYNVVKTQVV
                     NDRGIAICKSMLAWQKYGEGRTPASEHVKGDHFVGEYYVLFESKFQEEYKQFQLTEEG
                     KDCFDQRKKQEQSEKEFFTEYKNQYFNNQSTLGREARAMLLKWEEGDPAVRSLWSMMN
                     GWVLEGFEQTYTNLGVDFDKVYFESETYMLGKQIFLENIDNGIFYKKTDGSVWINLEN
                     QGLYHKLVLRSDGTSVYITQDTGMADIRYEEYQYDSCIYVVADEQDYHFKVLFEICKA
                     LKAPYADGLHHLSYGMVELPSGRMKSREGTVVDADDLIAEVINEATKNASERNELIDL
                     PQEEQNEINRKVGMAALKYHIIKVNPKKKMIFDPKESVDLQGHTGPYIQNAFVRISSV
                     LRKLESEVTTDFIAYNPINEDEKELLMQLSAYKGVISNAAESYDPSVLANYTYSLAKS
                     FHKFYHNHSILKAESPDAKSFRLSLSKVVATILADAMHILGIEMPEKM"
     gene            complement(3556..4017)
                     /locus_tag="PLE23_07010"
     CDS             complement(3556..4017)
                     /locus_tag="PLE23_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83048.1"
                     /translation="MNTRKKILKFSFAALLTYVLTIGYAQNVADITGDWYGVLTQNSG
                     GYKDTYNVELFLDKKGSAFTGIFVVKEKNLTSRMEVKGEWLTKSKVKLSDVRLLEHKE
                     PDGVNWCFKTYLLDFTITDKKPVLNGVWSGKTLTDKCVPGKVYLKKSLPKA"
     gene            complement(3986..4546)
                     /locus_tag="PLE23_07015"
     CDS             complement(3986..4546)
                     /locus_tag="PLE23_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HMX83049.1"
                     /translation="MRIISGRFKGRKFYPPADKWPTRPTTDYAKEGLFNILQNQLNFQ
                     DEQFLDLFGGTGNHSYEFISRGCNDVTYVDKHGPAVVFVKKVAAELGIDDKITIVKDD
                     VARFIKSSNRSYSYIFAGPPYPLPWLNTIPDLIFASSLLKKDGLFVLEHNPEHNFTNH
                     PNFVTSRNYGTTIFSFFEHKEENIEV"
     gene            complement(4537..5355)
                     /locus_tag="PLE23_07020"
     CDS             complement(4537..5355)
                     /locus_tag="PLE23_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HMX83050.1"
                     /translation="MTDEKLHILPFLAEDLDGADLFVGIEKTNLKLAVVNRVKNLSHI
                     LNFALNSGSAIENILPFNIRELNRCRKVSISLALDKFVIVPASFFDTRAATDYLDQAY
                     EIDRSEKVRYKLIKTMNAFMVYQQNEYFNKLVNIFPKTEIVLPEVDSFVQYINNNKER
                     VDLYLHFHNRSFILYLIIDNSVQFVQHYSFYEASDAVYYILNVLSCFKVDPAAIKAVV
                     SGNIMPYSDLYNTIYRFIGQVEWFNTSDSPLFAGQGFVENSHTIADLHCLALCV"
     gene            complement(5473..5841)
                     /locus_tag="PLE23_07025"
     CDS             complement(5473..5841)
                     /locus_tag="PLE23_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMX83051.1"
                     /translation="MFGSNKKTVKPELPQSPDSDLTCNIVSGTVIEGKMTTSENIRLD
                     GKIVGEVSCNKKMVMGETGSINGNMKAHNLFTKGKIVGDVNVGEVIHLMGDAFVKGNI
                     HAKKLLVEEGAKYDGECKIG"
     gene            5939..6433
                     /locus_tag="PLE23_07030"
     CDS             5939..6433
                     /locus_tag="PLE23_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83052.1"
                     /translation="MKNVFLLCLSIILIAACKNNSGTFDDSNSQQAVKLSNNPADFRA
                     KKDHLEKKLPPVEKRMPDFVPEDGKKNWFAVYSENNHHVCGIFRLPNETAWKEFMSTM
                     NFAEENEINKSLEWQTTEEWWVKRDVLANIKSRNLLAYKREDNTGSWYFAFNPRNFEV
                     YFWR"
     gene            complement(6536..6775)
                     /locus_tag="PLE23_07035"
     CDS             complement(6536..6775)
                     /locus_tag="PLE23_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83053.1"
                     /translation="MKNRLFMLMLAMICSVFVFAQNKPATATKPAATNEKAMNTQVKA
                     EAKPSDTPAKMEKKHTKKHRKAHKATKQNKVEEKK"
     gene            complement(6841..7116)
                     /locus_tag="PLE23_07040"
     CDS             complement(6841..7116)
                     /locus_tag="PLE23_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83054.1"
                     /translation="MKKLLFAAALMLSGSLVSFGQNAPHNKQATPTIHQQNTPVKAAA
                     TVQTAKHEATAEAKPVVKNEKMKSNRKHEASMKKIEKTEAKAPASVK"
     gene            complement(7223..>7481)
                     /locus_tag="PLE23_07045"
     CDS             complement(7223..>7481)
                     /locus_tag="PLE23_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMX83055.1"
                     /translation="WKKYAEDAKGAGHGGMDWFVINAFVESVKNEKPTPLDVYDHAVW
                     SSITPLSEKSIVLGSEPVEIPDFSRGRWMNKKNDFGLSDIW"
BASE COUNT         2134 a         1495 c         1392 g         2460 t
ORIGIN      
        1 accctatttc tgtaatatct tcgcccacga atcttttagt cctacagttt tgttgaatac
       61 cagtttttct gatgacgaat ctttgtcgag gcaaaaatag ccaagcctga agaactgata
      121 tgattttccg aaatcagcgt ctttcaatat tggttcaagc atagcccggg tatttactac
      181 cattgagtct ttgttgataa attccttgaa atcagtgtct ttgtcactgt tcggattttc
      241 gactacgaat aggcggtcgt acaatctgat ttcggcattt atggctgact ccggtaccca
      301 atgcagtgcg gttttgacct tgatacttga agtgtcttca cctgattttg aatcaggata
      361 atatttgaca tgaatttcag taattcgacc ttcactgtct gtgatgtgat tttcatactc
      421 tataatgtag gcgtatttaa gcctgacctt attaccgggt gataacctaa agtatccctt
      481 tggtgggtca agcataaaat cctctctttc aatatatatt actttaccaa acgagatatt
      541 acggtgacca gctgactggt cttcaggatt attctctgca gacaaatgtt cgcctgtgcc
      601 ctcataattg gtgataacca acttcaccgg atccattact accataactc gggtagctgt
      661 cttgttcagg tcttctctta cacaaaattc gagaaggcca atatctatga tgttttctct
      721 tttggcaacg cctattttgt cacaaaactc tacaatactc tttggcggat aacctcttct
      781 gcgcataccg ctaagtgtag gcatccgagg atcgtcccat ccactgacat ggttttcatt
      841 gaccaattga agaagcttac gcttgctcat gacggtataa ttcatattga gcctcgcaaa
      901 ttcatattgc ctgcttggga aaatctcaag tttttcgata caccagtcat acaatggtct
      961 gtgtggaata aactctaatg tacagattga atgtgtaatg gtttcgatag aatcagactg
     1021 accgtgtgcg aagtcgtaca tcgggtagat gcaccatttg tcgccggtac ggtggtggtg
     1081 agcatatttg attctgtata ggagcggatc gcgcatgtgc atattgggcg atgccatatc
     1141 gatttttgca cggagtacct tggatccttc aggaaattca ccgtccttca ttcgcctgaa
     1201 cagatctaag ttttcctcta ccgatctatc tctatgcggg ctgttttttc cgggttcagt
     1261 tggtgttcct tttagttcgg ctatctgttc tgcagttagg tcatcgacat aagccaggcc
     1321 tttaccaatg agttgtgtag cgtagtcata taaagtatca aaataatctg atgcgtaata
     1381 ctcgttagcc caatcaaacc cgagccattt gatatcttct ttaatagact caacgtattc
     1441 tgtatcttca gtcacaggat ttgtatcatc aaatctcaga ttggtttgtc cgttgtattg
     1501 ttttgccaga ccaaaattga tacatatcga cttggcatgg ccaatgtgca gatatccatt
     1561 aggctctggt ggaaaccttg tcagtaccct gccgtcatgt ttgccttcgg cgatatcatt
     1621 ttcgatgatt tcctgtaaaa aattttttga ttcaataggt tcagttattt ccataaatac
     1681 gaatattaca tcttttcagg catttctatt cccagaatgt gcattgcgtc agcaagaatg
     1741 gtagccacca ctttactaag actgagtctg aaagattttg catcaggact ttctgccttc
     1801 agaatgctgt gattgtgata aaatttgtga aaactcttgg caaggctata tgtatagttt
     1861 gccagtacag acggatcata gctttctgct gcattactta taacgccctt gtaagcagat
     1921 agttgcatta gcaattcctt ttcatcttca ttgatagggt tataagcaat aaaatctgtg
     1981 gtgacttctg actctagctt tcttagaaca gatgatatac gcacaaaagc attttgtata
     2041 tatgggccgg tgtgaccctg aagatcaaca gattccttag ggtcaaaaat catttttttc
     2101 ttaggattga ccttgataat atggtatttc aaagcagcca tacctacttt tctgtttatt
     2161 tcattttgtt cctcctgagg taaatcaatc aattcgttac gttcacttgc attcttagtt
     2221 gcttcattaa ttacctcagc tatcagatca tctgcatcta ccactgtacc ttcacgcgac
     2281 ttcatgcgac cggaggggag ctcaaccata ccatatgaca ggtgatgcaa gccatctgca
     2341 taaggtgcct ttagtgcttt acaaatttcg aacaaaacct tgaaatggta gtcttgttcg
     2401 tcagctacta cgtagataca tgaatcgtat tggtattctt catatctaat atcagccatt
     2461 cctgtatctt gcgtaatata aacggaagtg ccgtcagaac gaagtaccag tttatggtag
     2521 aggccttggt tttcgagatt gatccatacg ctgccatctg tttttttgta aaatatacca
     2581 ttgtcaatat tctccagaaa tatctgcttg ccgagcatgt atgtttcgga ctcaaagtaa
     2641 actttgtcaa aatctacacc taaattggta tatgtttgtt caaatccctc cagcacccaa
     2701 ccgttcatca tagaccaaag actccttact gccggatctc cttcctccca tttcaacaac
     2761 atggcacgtg cttcccggcc taaggtgctt tgattgttaa aatattggtt tttatattct
     2821 gtaaaaaatt ccttttcaga ctgctcctgt tttttacgtt ggtcgaagca atctttgccc
     2881 tcctctgtaa gttggaattg cttgtattct tcttgaaatt ttgattcgaa cagaacataa
     2941 tattctccga caaaatgatc tcctttgaca tgctcagaag caggtgttct accttcaccg
     3001 tatttttgcc atgccaacat acttttacag atagcgattc cacggtcatt tactacttgg
     3061 gttttcacta cattataacc gagtctttgt aacaatcggt agatgctcca acctagtagt
     3121 atattgcgaa tatgaccaag atgtaatggt ttatttgtgt tgggtgaggc atattcaact
     3181 agcacagtct tatcattctc aggaaacatt ttctgaaaat cggttgcgag caatgcttcg
     3241 agagaatctt tccaaaattg atcattcatc acgatattca gaaatccacc aattgcttca
     3301 aaacccaact caggaaaaag atttttaagg cctgattgga tttcgttacc tagttcggtg
     3361 ggatttttac cggctttttt ggcaagtggg aaaatgagta ctgtatagtc acctttgttc
     3421 tgagttttat tcggcgatat ctgtatgtct gactctacaa atgccttgtc agcatacatt
     3481 gatttcagaa tacttagagt attagattgt attttgtgca atagattcat agtcaaatga
     3541 taatattaga atacttcatg ctttaggtaa tgattttttc aaataaacct taccaggtac
     3601 gcatttatcg gttaatgttt tgccagacca aactccattc agtacgggtt ttttatctgt
     3661 aatggtaaaa tcaagtaaat atgttttgaa gcaccagttg acaccatccg gttccttgtg
     3721 ttctaatagc ctaacgtcag aaagcttgac cttggacttt gtcaaccact ctcctttcac
     3781 ctccatcctg gatgtcaggt ttttctcttt tactacaaaa atacctgtaa aggctgagcc
     3841 ttttttatca agaaagagct caacattata ggtatcttta tatccacctg agttttgagt
     3901 aagaacacca taccagtctc cggtaatatc tgcaacattt tgtgcatacc cgatggttaa
     3961 cacataagtc aaaagtgctg caaaactaaa cttcaatatt ttcttccttg tgttcaaaaa
     4021 aactaaaaat tgtggtgcca taattacgtg aagtcacaaa gttaggatga tttgtgaaat
     4081 tatgctcagg attatgctcc aaaacgaaca aaccgtcttt tttcaataat gatgatgcaa
     4141 aaataagatc tggaatggta tttagccacg gcaatggata tggtgggccg gcgaagatat
     4201 aagagtaact tctgttggag gatttgataa aacgagctac atcatcctta acaattgtta
     4261 tcttgtcatc aatgcccagt tctgcagcca ccttcttaac aaatactaca gccggtccat
     4321 gtttgtcaac ataagtaaca tcattacaac cacgagaaat gaactcgtag ctgtgattgc
     4381 cggtaccacc aaataaatca aggaattgtt catcttgaaa attcagctga ttctgcagaa
     4441 tattgaataa accctcctta gcataatctg tagttgggcg agttggccat ttgtcggcag
     4501 gtggatagaa tttcctgcct ttgaatctac cgcttattat acgcataatg caaggcagtg
     4561 aagatcagcg attgtgtgcg aattctcgac aaatccttgt ccggcaaata gcggtgaatc
     4621 gcttgtattg aaccattcga cctgacctat aaacctgtaa atggtattgt acagatctga
     4681 gtacggcatg atattaccag acacaactgc tttgatagct gcaggatcga ctttgaagca
     4741 cgagagaaca ttcaaaatat aatatactgc atcagaagct tcatagaaag aataatgctg
     4801 tacaaattgt acactattgt caatgatgag ataaagtata aatgaacggt tgtgaaaatg
     4861 caggtacaaa tctacccgtt ccttattatt atttatatac tgaacgaaag aatctacctc
     4921 aggcaacaca atttcagtct ttggaaatat gttgacaagc ttattaaaat actcattttg
     4981 ttgataaacc ataaaagcgt tcatggtctt gataagtttg tatcgtactt tttcgcttcg
     5041 atctatttca taggcttggt caagataatc ggtagcggca cgcgtatcaa aaaatgaggc
     5101 aggtacaatt acgaatttgt ctaaggcaag ggatatagat accttgcgac aacggttgag
     5161 ttctcggata ttaaaaggta aaatattctc aattgcagaa ccggagttca atgcaaaatt
     5221 cagtatgtgt gataaatttt tgacacgatt aacaactgcc agtttcaggt tggtcttttc
     5281 gattccaaca aacaaatcgg caccatcgag gtcttcagct aaaaatggca atatatgaag
     5341 tttttcgtct gtcatgttat tcagcttggc aatattcggc caatattact caatttcata
     5401 atattaacaa acaaagataa gccttacctc tgatatagta atgtgaaaat ccagaaaaaa
     5461 gagacggagg tattatccta ttttacattc gccgtcatat ttggcgcctt cctcgaccaa
     5521 caatttcttc gcatgaatat ttcctttaac aaaagcatca cccatcaggt ggataacttc
     5581 acccacattt acatcgccta ctattttacc cttagtaaac agattgtgcg ctttcatatt
     5641 tccgttgatg ctaccggttt cgcccattac cattttttta ttgcaagaca cttcgcctac
     5701 aatcttgcca tcgagtctga tgttttcgga agtagtcatt ttgccttcga tgaccgttcc
     5761 gcttacaata ttgcaagtga ggtcagagtc agggctttga ggtaattcgg gctttacggt
     5821 tttcttattg gatccaaaca taacaatatt tttaaaatac acagaaaaca ttatgccaaa
     5881 caggctgaaa ccttcagcaa ttttatccgt ttttaaaagt ccttcaaata ttacaggaat
     5941 gaaaaatgtt ttcttactct gcttatcaat tattctgatt gctgcctgca aaaataactc
     6001 aggtacattt gatgattcca acagccaaca agcagtaaaa ttgagcaaca atccggccga
     6061 ttttcgtgcc aagaaagatc atctggaaaa aaagctccca ccggttgaaa agcgtatgcc
     6121 cgatttcgtt ccggaagatg gcaaaaagaa ttggtttgcg gtatatagcg aaaacaacca
     6181 ccatgtctgt ggtattttca gacttccgaa tgaaactgcc tggaaagaat ttatgtcaac
     6241 catgaatttt gcagaagaaa atgaaataaa caaatcactt gaatggcaga ctaccgaaga
     6301 atggtgggta aaaagagatg ttctggccaa tattaagagc cgcaatttgc ttgcctacaa
     6361 aagagaagac aacactggta gctggtactt tgcatttaac ccgagaaatt tcgaggttta
     6421 cttctggaga tagacaaatt ataatcctga taaaaaaaag ggcatcagca cgctgatgtc
     6481 cttttttcgt tgtaaggcag aagcttctat tctgcgttgt caaaatgttt gtttatcatt
     6541 tcttttcttc tactttattc tgcttggttg ctttgtgtgc ttttcggtgt ttttttgtat
     6601 gcttcttctc cattttggca ggagtatccg aaggtttggc ttcagccttt acttgagtat
     6661 tcattgcttt ttcatttgtg gctgccggct tcgtagcagt tgcaggtttg ttttgtgcaa
     6721 atacaaaaac tgaacatatc attgccaaca ttaacataaa caatctgttt ttcatgattt
     6781 aaaaatttga tggtgaaaaa atagtgttaa aaaatgaatc taattggtga attgaaaaaa
     6841 ttacttaact gatgcaggcg ctttagcttc agtcttttca atctttttca ttgaagcttc
     6901 atgtttgcga tttgatttca ttttctcatt cttcacaaca ggctttgctt cggcggttgc
     6961 ttcgtgtttg gcagtttgca ctgtagctgc agccttaaca ggtgtgtttt gttgatggat
     7021 agttggcgtt gcctgtttgt tgtgtggtgc gttttgacca aagctcacaa gtgaacctga
     7081 caacatgagt gcagcagcaa acaataactt tttcataata attttggttt aggtgataaa
     7141 ataaaattga aagcattcgc attaaaaacg catcctaaaa ccatttatta tttgtgccaa
     7201 tgttatggtt tggttaaaaa aattaccata tatcagacag cccaaaatca ttctttttat
     7261 tcatccaacg gcctcttgaa aagtctggaa tttctaccgg ttcactacca agtactatgg
     7321 atttttcgga taatggtgta atgcttgacc agactgcatg atcataaaca tccagtggag
     7381 taggcttttc atttttaaca gattcgacaa aagcattgat aacaaaccaa tccataccgc
     7441 catgcccggc acctttggcg tcttcagcat attttttcca c
//
LOCUS       DAOQQY010000122         7379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4043_length_7379_cov_5.512835, whole genome shotgun sequence.
ACCESSION   DAOQQY010000122 DAOQQY010000000
VERSION     DAOQQY010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4043_length_7379_cov_5.512835"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="PLE23_07050"
     CDS             <1..1210
                     /locus_tag="PLE23_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83056.1"
                     /translation="DVAYGTGGNFGSAISADQVNLQTQNVEVELIPAKYWTVNVGLQR
                     MYDTPHDLYRTVFDKFTSTGYRLAYWGTDGVGISVRRETDYSKLKGGFYKLYENNVEL
                     NDDVTLFELNSQLNINRKWNLGGSIYWVRDRSSGKGGVSILGQGFASNLTSYNGTYRF
                     ALNADPYRADIFWVGSHFSRNEDFMLDRWLLSGFINLNAGSISQRNPDQSFRKTADIL
                     GVGANLRAGYRYGQTAGDAVTADLLFTTGDDNDLSDKKYSGVITGNTWGSPAGIFIGH
                     GAYLLFPHGNVVNRYVAAVADISNMGLGVAGGTLNVAHDFIPNKLHTKLGTAAAFAPV
                     APAGGGNFMGLEVNGKVGYDFGAFLTIEAHAAYLSLGDFYDSPVVNGGVPIRPVNPWT
                     GFISLKWLIF"
     gene            1214..2164
                     /locus_tag="PLE23_07055"
     CDS             1214..2164
                     /locus_tag="PLE23_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMX83057.1"
                     /translation="MKKSAKYIIIAIICLVQIHGNAQHYTSVMKSMKDLVYNYDSKFI
                     SLNNDIELSYIEEGSGNETILFIHGLGSYIPAWNKNVESLKDFYKCIAIDLPGYGKSS
                     KENYSGTMEFYADVIHEFCQKKNLGKVILAGHSMGGQISMVTAIKYPTLVKKLILIAP
                     AGFEVFNKGEKQWFRDVMTVDGVRLTTVENIRLNLAYNFYQMPADAEFMVKDRIEMRG
                     ATDFPAYCYAITQSVKGMVDQAVFDFLPDIKQPALCIFGENDNLIPNRFLNGGPTRKY
                     AEKGASRMPNCSLKLIPKAGHFVMYEKSEEVNSIIRGFLR"
     gene            complement(2255..2599)
                     /locus_tag="PLE23_07060"
     CDS             complement(2255..2599)
                     /locus_tag="PLE23_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83058.1"
                     /translation="MKLKMMILIVSSVLVLAMSCTKETAVCNTEKVTYNNQIKTIMAG
                     CTYPGCHNSGSVNGGLTSYQECINFPKKDKLLSAVNWDGKAVIMPPSKNKISDCDISK
                     MAKWIDSGYPEN"
     gene            complement(3237..3581)
                     /locus_tag="PLE23_07065"
     CDS             complement(3237..3581)
                     /locus_tag="PLE23_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83059.1"
                     /translation="MKLKNVVLMATAAALSVAACTKESTVCDTEKVTYNNQIKTIFAG
                     CTDAGCHNSSSVNGSLANYQDAVNFPKKSKLIGALKWEEGFVQMPSGKGKLSDCDISK
                     VEKWIDSGYPEN"
     gene            3844..4287
                     /locus_tag="PLE23_07070"
     CDS             3844..4287
                     /locus_tag="PLE23_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83060.1"
                     /translation="MKTIRKFLLPVALMLIFIASCDKDDTKTTKDYLISKSCWYLSKA
                     ETQNPDQTWSDVTNLIYKPCDFDNCMNLKADGTFTVDEGATKCSPAAPQTNNQGNWSL
                     SPDEKQVVLIDQNKVTTTYQIVNISAAQLSVTTNILGVQTRITFK"
     gene            complement(4362..5501)
                     /locus_tag="PLE23_07075"
     CDS             complement(4362..5501)
                     /locus_tag="PLE23_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMX83061.1"
                     /translation="MTDHTENLKMIEQSARDCAETYIRPHVMEWDESQHFPVDAMKIL
                     GQHGFLGVLVPEEYGGSGLGYQEYITVIGEIAKVCGSVGLSVAAHNSLCTGHIMAFGN
                     EEQKKKYLPLLATGEWIGGWGLTEPNTGSDAGRMQCVAKKDGDYYVINGTKSWITHGI
                     SSNVCVLIARTGELLDSHGMSAFIIERGTPGFSYGKKENKLGMRASETAELIFDNCRV
                     HKSQLLGNEGDGFVQAMKILDGGRISIAALALGIAKGAYEASVKYAQERQQFGKPIAS
                     FQGISFKLADMATKIEAAELLTRKAGDMKNQGLKMTKEGAMAKYYASEISVEVSTDAV
                     QIFGGYGYTKDFPVEKFYRDSKLCTIGEGTSEIQKIVIAREVLKE"
     gene            complement(5530..6813)
                     /locus_tag="PLE23_07080"
     CDS             complement(5530..6813)
                     /locus_tag="PLE23_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMX83062.1"
                     /translation="MNPPLAERLRPHTLEQYAGQEHLLGPGKVLRNAIENNQLPSFIL
                     WGPPGVGKTSLAFLISKICERPFFTLSAIQAGVKDIREVFEKAKKERFFNRPNPILFI
                     DEIHRFSKSQQDALLGEVEKGTVTLIGATTENPSFEVIPALLSRCHVYVLKHLEHEQL
                     ISLIENAVKTDYELKQKHFSFKSYDAIIRLSGGDARKLYNIIELLANTILPGESIEVT
                     DELVMEKVQQHLSVYDKGGEMHYDVISAFIKSIRGSDPNAAVYWLARMVEGGEDPDFI
                     CRRLIILASEDIGLANPNALLMAKTAHDAIKMVGWPEGRIILSQAAIYLATSPKSNSA
                     YEAIENALYTVRSQPNAPVPLSLRNAPSALMKKLDYGKDYKYAHGYEGNFVQADFLPE
                     QLKGKILYRPGKNSSENKILEQLIAHWDGIYKYNQ"
     gene            complement(6815..>7379)
                     /locus_tag="PLE23_07085"
     CDS             complement(6815..>7379)
                     /locus_tag="PLE23_07085"
                     /inference="COORDINATES: protein motif:HMM:NF020691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leukotriene A4 hydrolase C-terminal
                     domain-containing protein"
                     /protein_id="HMX83063.1"
                     /translation="TITTPVFLDYLQKNYLNKFPDFDPKIDLWLNQPGIPAQATPPVS
                     VKFDALDKMIASYRDGKISEKDFNHNFSSHEWVYLIRKLPDTLTDKQFENFNKLYQWS
                     KSGNNEIKAAWIEKATITGHGKAILDDTSAFLESVGRRKYLEPIYTALKTKGLGVEAK
                     EIYAKARPLYHYVAVRTIDELLNFHPK"
BASE COUNT         2059 a         1482 c         1474 g         2364 t
ORIGIN      
        1 cgatgtggct tatggcacgg gtggcaattt cggttctgcc atctctgccg atcaggtcaa
       61 tcttcagact caaaatgtag aagttgaact tattccggcc aaatactgga ccgtaaacgt
      121 aggcctccaa aggatgtatg atacaccgca tgacctatac cgcacagtat ttgacaaatt
      181 taccagtact ggttatcgtt tggcttattg gggtacggat ggtgtaggca tttcggtaag
      241 aagagagact gattattcta agctgaaggg cggattctac aaattgtatg aaaataacgt
      301 cgaactaaac gacgatgtta cactgttcga actcaacagc cagttgaaca ttaaccgcaa
      361 atggaatctt ggcggtagta tctattgggt gcgcgaccgg tcatccggta agggtggcgt
      421 gagtattctc ggacaaggct ttgccagtaa ccttaccagc tataatggta cataccgctt
      481 cgcactgaat gccgaccctt atcgtgcaga tattttctgg gtaggcagcc attttagccg
      541 aaatgaagat tttatgctcg acagatggtt gttgtcaggc tttatcaatt tgaatgcagg
      601 tagtatatca caacggaatc cggatcaatc ctttagaaaa acagctgata ttctcggtgt
      661 aggtgccaat ctgcgtgccg gatatcgata cggacagact gcaggtgatg ccgttactgc
      721 agatctgcta tttacaacag gagatgataa tgacctatca gataaaaaat attcgggtgt
      781 tatcaccggt aatacctggg gtagcccggc aggtatattt ataggacacg gtgcttactt
      841 gttgtttcct catggcaatg tagtaaaccg gtatgtggca gcggtggcag atatttccaa
      901 tatgggtctt ggtgtagcag gaggtacact caatgtggcc cacgatttta taccgaataa
      961 actgcataca aaactcggta cagctgctgc ctttgctccg gtagcacctg caggaggagg
     1021 caattttatg gggcttgaag tcaatggtaa ggtaggatac gacttcggtg catttcttac
     1081 tattgaagca catgcagcat acctgagcct gggcgatttc tacgatagcc ctgtagtcaa
     1141 tggtggcgta cccattcgtc cggttaatcc atggacaggt tttattagtt tgaaatggtt
     1201 aattttttaa agcatgaaaa aatcagcaaa atatatcatc attgcaatta tttgtttggt
     1261 acagatacat ggtaatgctc aacattacac atcagtgatg aagagtatga aagatttggt
     1321 atataattat gactcaaaat tcatttcact caataacgat attgaacttt catatataga
     1381 agaaggctcg ggcaatgaga ctatactgtt tatacatggt ctgggtagtt acattccagc
     1441 atggaacaaa aacgtagagt cactcaagga tttttataaa tgtattgcca tcgatctgcc
     1501 aggttatgga aaatcttcca aagaaaacta tagtggtaca atggagtttt atgcagatgt
     1561 cattcatgaa ttttgtcaga agaaaaacct gggcaaagta atacttgccg ggcattcaat
     1621 gggtgggcag atcagtatgg tcaccgcaat caaatatcct acacttgtca aaaagctgat
     1681 acttattgca ccggccggat tcgaagtgtt taacaaaggt gaaaaacaat ggtttagaga
     1741 tgtaatgacc gtggacggcg tacggttgac tactgtggaa aatatacggc tcaatctggc
     1801 ctacaatttt tatcaaatgc cggctgatgc cgaatttatg gtgaaagata gaatcgaaat
     1861 gcgcggcgct accgattttc cggcatactg ctatgctatt acccaaagtg tcaaagggat
     1921 ggttgaccaa gctgtatttg attttttgcc cgacatcaaa caacctgcgt tgtgtatttt
     1981 tggtgaaaat gacaacctga tacccaatcg atttctcaac ggtgggccca ccagaaaata
     2041 tgctgaaaag ggagcttcca gaatgcccaa ctgcagcttg aaattgatac ctaaagcagg
     2101 tcattttgta atgtatgaaa aatcggagga agtaaacagc attatcagag gatttttgag
     2161 ataaaaaatg catcccgata ctgttggtta gatcgggatg caatctgcat tttagaggtt
     2221 tttagaagaa cattttatca gccaagctga aattttagtt ttcaggatag cctgaatcaa
     2281 tccattttgc cattttcgaa atatcgcagt cgctgatttt gtttttactc ggaggcatga
     2341 ttaccgcttt gccatcccag ttaacagcgc tgagcaactt atctttctta ggaaaattta
     2401 tgcactcttg atagctggtc agaccaccat taacagaccc actattgtga cagccggggt
     2461 aagtacagcc tgccattatg gttttgattt ggttgttgta ggtgactttt tctgtgttac
     2521 agactgcggt ctctttggtg caagacatag ccaacaccaa aacgcttgat acaataagta
     2581 tcatcatctt aagtttcata atagtgtagt gttttgttgg ttaaaaagaa tatttcagtt
     2641 ttctaaaatg caatgcaaat atagtattga atatttgtta aaatattaaa atatttgttt
     2701 actttattta tatttcaaat aaaatatagt gtatattgtg ttaattaaat ataaaagttt
     2761 gtttaacaat caagcttatg attttctaat ctgaattggc aatgttgtac atagcaactg
     2821 cactttattt attaattatt ttcgtcatac attttgtttt ctaatattct gtattattta
     2881 gcaaaaataa tttctattta tataaaatat tcttttgcat gacttagata aatgaaaaat
     2941 atttctttga atctaaaatg acttactata cttgaccgat ctaacgcata gtgcctatat
     3001 acagaagatc attttcaaaa gatgattttt gagcgaagat gaagcaagat aggtgcagta
     3061 ggtttttggt gctcaagtat ttcgcataag gattacgcct accattagga tattaaagga
     3121 acttaataaa gaagtgcgtg tactgcggta atgaatgtat ttgccgctgt gcacgcacct
     3181 tgataacatg atattgtggc caccattcaa gtcaaatggt agagcttacg ttatatttag
     3241 ttttcaggat agcccgaatc aatccatttt tctactttcg aaatatcaca atcacttagt
     3301 tttcctttgc ctgatggcat ttgcacaaag ccttcttccc acttcagagc accaattaat
     3361 ttcgattttt ttggaaaatt aacagcatcc tggtaattgg ccaaactacc attgacagag
     3421 ctgctattgt ggcagcctgc atctgtacaa cctgcaaaaa ttgttttgat ctggttattg
     3481 taagttactt tttcggtatc gcaaacagtg ctttcctttg tacaagctgc gacactcaat
     3541 gcggctgcgg tagccatcaa cacaacattc tttaatttca taatagtgac atttattcgt
     3601 ttggttaaaa tttttgtgta tagtttgcca gtcttaacac acaactcacc tgatttgttg
     3661 tacaaacagg ccattttttt gtaaattgtc aagaacttac ttgtgtatct tttctatcaa
     3721 gagacgatga cttcagattg ccccgaaata ccgctaactg taaaatattt catttttcca
     3781 caaaataatt gttatttaca gacgttattt gtatcattac attcattacc taaaatatca
     3841 cctatgaaaa caatccgcaa atttttatta cctgttgctt taatgctcat ttttattgcc
     3901 tcgtgtgata aagatgatac caagaccaca aaagactacc ttataagcaa atcatgctgg
     3961 tatctttcca aagctgaaac tcaaaatccg gatcagacat ggtcagatgt cacaaacctg
     4021 atatataagc cttgtgattt cgataattgc atgaacctta aagctgatgg cacatttacg
     4081 gttgatgaag gagccacaaa atgttcacct gcagcaccac aaaccaacaa tcaaggtaac
     4141 tggtctttaa gtccggacga gaagcaggtt gtactcattg atcaaaacaa agtaacaacc
     4201 acttatcaga ttgtgaatat atcagccgcc caactaagtg tcactaccaa tatacttggg
     4261 gtacagactc gaatcacatt taagtaatca ggttttttta taaataaaaa tgcggttaac
     4321 ttgagcaaag ttaaccgcat ttttgtatgc ttcatgtagt attattcttt caatacttcc
     4381 ctcgctatta ctatcttttg aatctctgac gtgccttcac caatggtaca tagttttgag
     4441 tcgcggtaaa atttttcgac cggaaaatcc ttagtatatc catatccgcc aaatatctgc
     4501 acagcatctg ttgacacttc taccgatatt tcggatgcat aatattttgc cattgcgcct
     4561 tctttggtca ttttcaatcc ttggttcttc atatcgccag cctttcgtgt caataattcg
     4621 gctgcttcta tcttggttgc catgtcggca agtttgaagc ttatgccttg aaaactggct
     4681 atcggctttc caaactgttg acgttcctgt gcatatttta ccgatgcttc gtatgctccc
     4741 tttgctatac ccagtgcaag tgcagctatg cttattctac ctccgtccaa tattttcatt
     4801 gcctgaacaa aaccatctcc ttcattaccc aacaattgtg acttgtgcac acggcagttg
     4861 tcaaagatta attctgctgt ttcggatgca cgcatgccaa gcttattttc tttcttgcca
     4921 tagctaaatc cgggagtacc tcgttctatg atgaatgcac tcataccatg actgtcgagc
     4981 aactctcctg tacgggctat gagtacgcaa acattacttg atataccgtg agttatccaa
     5041 ctcttcgtgc cgtttataac gtagtagtca ccatcctttt ttgctacaca ttgcattctg
     5101 ccggcatcgc ttcctgtatt tggctctgtg aggccccatc caccaatcca ttcacctgta
     5161 gccaatagtg gcaggtattt tttcttttgt tcttcattgc caaatgccat gatatgcccg
     5221 gtacacagcg aattgtgggc tgctaccgac aagccgaccg aaccacatac ttttgctatt
     5281 tcaccgatga ccgtaatgta ttcctgataa cccagtccgg atccaccata ttcttcaggt
     5341 actaacacgc ccaagaagcc atgctgcccc agtattttca ttgcatctac cggaaaatgc
     5401 tgcgactcgt cccattccat tacgtgagga cgaatgtagg tttcggcaca atctcttgcg
     5461 ctctgttcaa tcatttttaa gttttcagtg tggtctgtca tacctgtttt tttacaaata
     5521 taaagtattt tattgattat atttataaat accgtcccaa tgagcaataa gttgctcaag
     5581 tattttattt tcagatgaat tttttcccgg acgatacaat attttgccct tcaactgttc
     5641 tggcaagaaa tcagcttgta caaaattacc ttcatagcca tgtgcatatt tgtaatcttt
     5701 tccataatca agcttcttca tcaatgcaga aggcgcatta cgcaatgata aaggcactgg
     5761 tgcattgggc tggctcctta cggtgtaaag cgcattttct atagcttcat aagcagagtt
     5821 gctcttaggt gaagtagcca gatagattgc agcttgtgat aaaatgatcc tcccttccgg
     5881 ccatccaacc attttgatgg catcatgtgc tgttttggcc ataagcaagg cattcggatt
     5941 ggcaagaccg atatcttcac tcgccaggat aatcaacctt cggcagataa agtcgggatc
     6001 ttctccacct tctaccatgc gtgccagcca atatactgct gcattggggt cactgccgcg
     6061 gatggatttt atgaaggctg atattacatc ataatgcatt tcgcctcctt tgtcatacac
     6121 ggacagatgc tgctgtactt tttccatcac gagctcgtcg gttacttcta tagattcgcc
     6181 cggaagaatt gtatttgcca gcagctctat aatgttgtat agcttacggg catcaccacc
     6241 acttagtcga atgatagcat cgtaagactt aaatgaaaaa tgcttttgct tgagttcgta
     6301 atcggttttg actgcatttt ctatgagcga aatgagctgt tcgtgctcca gatgtttgag
     6361 tacatataca tgacacctcg acaatagggc aggtataacc tcaaaagatg gattctctgt
     6421 agtggcgcca atgagtgtca cggttccttt ctctacttcg cccaataagg catcttgctg
     6481 tgacttgctg aagcgatgga tttcatcaat aaacaggatc ggatttggtc tgttgaaaaa
     6541 tctttctttt tttgcttttt caaatacttc acgaatgtct tttacaccag cctgaatggc
     6601 gctcagtgta aaaaaaggcc tttcacaaat ttttgaaata agaaaagcca gtgaggtttt
     6661 tcccacaccg ggtggccccc aaaggatgaa ggacggtagc tgattgttct cgatagcatt
     6721 gcgcaatact tttcccggcc ccagtaggtg ttcctggcct gcgtattgct cgagtgtatg
     6781 tggtctaagc cgttctgcta aaggcggatt cattttattt tggatgaaaa ttcagcaatt
     6841 catcaatagt acgaactgct acataatggt agagtggtct tgcttttgca taaatttctt
     6901 ttgcttcaac cccgagtcct ttggttttca atgcggtata gattggttca aggtattttc
     6961 gtctgcctac cgattcaagg aatgccgagg tatcatcaag tatagctttg ccgtgtccgg
     7021 taattgttgc tttttctatc catgcagcct ttatctcgtt attgcctgac tttgaccatt
     7081 ggtataattt attgaaattt tcgaattgct tgtcagttaa ggtgtcgggt agtttgcgta
     7141 tcagataaac ccattcgtgt gaagaaaaat tgtggttgaa gtctttttct gaaattttcc
     7201 catcccggta gctggctatc attttatcaa gcgcatcaaa ttttacagaa acaggtggtg
     7261 tggcttgtgc gggaatgccg ggttggttga gccacaggtc tatttttgga tcaaaatccg
     7321 gaaatttgtt taaataattt ttttgcaaat agtctagaaa tacgggtgtg gtgatggtc
//
LOCUS       DAOQQY010000123         7376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4049_length_7376_cov_6.689796, whole genome shotgun sequence.
ACCESSION   DAOQQY010000123 DAOQQY010000000
VERSION     DAOQQY010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4049_length_7376_cov_6.689796"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLE23_07090"
     CDS             complement(<1..385)
                     /locus_tag="PLE23_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMX83064.1"
                     /translation="MLVGQYDGKVKTLFRYIDQCEKIDRFKEVWILYNDVEKLFEDFN
                     SLYQRMDAAGGLILNAQNEILLMYRLGMWDLPKGKIEDDESVEEAAVREVSEETGIEV
                     QPTIFKLCDTYHTFRINQKRILKKTY"
     gene            495..1151
                     /gene="pyrE"
                     /locus_tag="PLE23_07095"
     CDS             495..1151
                     /gene="pyrE"
                     /locus_tag="PLE23_07095"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMX83065.1"
                     /translation="MVSTQATAFSDYLLQIKVIQLNPQNPFTWASGIKSPIYCDNRLV
                     LSYPAIRKTAIDGLEELSRLFSNIDVIAGVATAGIPHGALLADRLNLPFIYVRSKPKE
                     HGRQNQIEGAYNSGQRVVVIEDLISTGGSSLEAIEALRAEGLEVVACLALFTYQFEKS
                     VTRFAESNVPLHTVTDYSHILTRAVEHTYITEQQRAVLSEWKDNPEGWYEKNFNSTTN
                     "
     gene            1258..2337
                     /locus_tag="PLE23_07100"
     CDS             1258..2337
                     /locus_tag="PLE23_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HMX83066.1"
                     /translation="MSIISPVAETFLEKYLNNASPTGFEYPGQKLWIEYIKPFIDEWH
                     SDNYGTVYGVINPGQDYKVVIEAHADEISWFVNYITDDGFINVIKNGGSDHLIAPSKR
                     VNIHTKNGFVKGVFGWPAIHVRNGKEPSLTPTIENIFIDIGAKNKEEVLEKDIHVGTV
                     ITFEDEFMMLNNKYYVGRALDNRIGGFCIAEVARMLKDKNIKLPYTLYIVNSVQEEIG
                     LRGAEMIAHRIKPDVAIITDVTHDTNTPLMTKSKLGDIKSGNGPTVTYGPAVHNKLLE
                     LIIGAATENEIPFQREAASRSTGTDTDAFAYSNAGVPSALISLPLRYMHTTVEMANKS
                     DVENVIKLIFEALQKIERNHNFNYF"
     gene            2561..3025
                     /locus_tag="PLE23_07105"
     CDS             2561..3025
                     /locus_tag="PLE23_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HMX83067.1"
                     /translation="MINIELGLLKYSPQLVTVKSPDKILVRDPVRKILTILTPEETVR
                     QLLILFLFEKYSHLKNLISVEKELKINFRKKRFDLLIYDRQMKPFLLVECKAPSIKIT
                     QAVFDQVSWYNVALNAPFLLVTNGISTYCAKIDFDKKTYNFSDYLPLDFENL"
     gene            3079..4041
                     /locus_tag="PLE23_07110"
     CDS             3079..4041
                     /locus_tag="PLE23_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMX83068.1"
                     /translation="MKQEKILIIGASGQIGSVLTHTLSDIYGENNVIATDIRQPTDHH
                     GHFEILDVLNTQALGDIVAKYGITQIYHLAAILSAKGETMPMFAWQFNMTSFFNVIDV
                     ARQFKISKIFFPSSIAAFGPSTPKYNTPQATIMIPETVYGISKSCAEDWCNYFWLKEK
                     MDIRSVRYPGIIGYQSLPGGGTTDYAVNIYHKAVKSEPFECFLKADTALPMIYMDDAI
                     RATIELMEAPAENIKIRTSYNLASMSFSPQDIAESIKKQVPDFSISYNPDFRQAIAES
                     WPASIDDSEARKDWGWRPNFDLDAMTADMLAHLSEMYSKEIVHH"
     gene            4060..5247
                     /gene="kbl"
                     /locus_tag="PLE23_07115"
     CDS             4060..5247
                     /gene="kbl"
                     /locus_tag="PLE23_07115"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HMX83069.1"
                     /translation="MFNEVRDQLAAELAAIEEQGLYKRERIITTPQAAHISTVEGGDV
                     LNFCANNYLGLSSHPEVTGAAKHAIDTHGYGMSSVRFICGTQDIHKELEKKTAEFLGM
                     EDCILYAAAFDANGGLFEPILNEEDAIVSDELNHASIIDGIRLCKAKRFRYKNNDMND
                     LEAKLKEAQGSRRILIATDGVFSMDGIVAQIDKICDLADKYNAMVMVDECHSTGFMGK
                     TGRGTHELCNAMGRVDIITGTYGKALGGASGGFTAARKEIVDMLRQRSRPYLFSNTLA
                     PSIVGGSLKVLELLSASTHLRDKLETNTQHFRKGMTEAGFDIVPGFHPITPVMLYDAK
                     LAQDFAARLLKEGIYVIGFFYPVVPQGKARIRVQVSAGHEIADLDKAIAAFTKVGKEL
                     GVI"
     gene            5356..>7376
                     /locus_tag="PLE23_07120"
     CDS             5356..>7376
                     /locus_tag="PLE23_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HMX83070.1"
                     /translation="MKYSLTIFIVLAFTLPSFAQPSPFIQVDQFGYKPLAEKVAVLSN
                     PIIGFNSNLSYQPPAMMEVRRAVTNETVLVGDVNLWNGGAVHNQSGDMGWWFDFSGVQ
                     DTGHYYIYDPLNNESSGIFRIAENPYIDVLKAAGRMYFYNRCNDEKALPFAGEKWQDG
                     MNFLHPLQDANCRYVYDKTNAALEKDLRGGWFDAGDYNKYVSFTFAPLHDLLFAYEEN
                     PQAFGDDWNIPESKNGFPDIIDEVKWELDWLMRMTNADGSAYNKMGSIAYDQNALSPP
                     SANTDPRYYGPTCSSASITIASVLSHAAVVFKKFKGLEGYAVQLQQTAETCFNYCLPF
                     YLNNNWETNCDDGSINSGDCDESVQNQLEIFITACVYLYELTGDETYHDYFKANYLKS
                     RPMSNSYWSGDYPTIQDALIRYAWKLQNTDKNVQLDIQSDLKEAIANNWNDFFGWNDL
                     DLYRAFVPDWTFTWGGNKPNACYGILSFNISRYKMVSDTSGFVKKGNEQLHYFHGVNP
                     LGMVWLSNMYPYGAFRCANQIYHSWFKDGSIYDHALNSPNGPAPGFMVGGPNIYYSIT
                     SISPPANQPAMKSYLDFNNDWPDNSWEITEPSIYVQAAYIRLLAYHVTKDITTKITDQ
                     TTNTAFHISPNPANQWVALSGINFPADIVILDVHGRTVFEKSDIAASES"
BASE COUNT         2228 a         1490 c         1526 g         2132 t
ORIGIN      
        1 agtatgtttt tttaagaatc cgtttttggt tgattctgaa tgtgtggtaa gtgtcgcata
       61 gcttaaagat tgtcggttgc acctctatcc cggtttcttc actcacttca cgtacggctg
      121 cttcttcgac cgattcgtca tcttcaatct tgccctttgg gagatcccac ataccaagtc
      181 tgtacataag tagaatttca ttttgtgcgt tgagtatcaa gccacctgct gcatccatcc
      241 tttggtacaa actgttgaag tcctcaaata atttttctac atcattgtat agaatccaga
      301 cttctttgaa cctgtcaatt ttctcacatt ggtcaatata tcggaaaagt gtcttaactt
      361 tgccgtcata ttggcctacc aacacggaag catctttttt ttctaaaatt tcccgtaagg
      421 gtgcatttgt tagaattaaa ggagtttgat tgatgtatat tttgtacatt tggcagattt
      481 ttctaaaaca tactatggtt tcaacacagg caacagcctt ttcagattat ttattgcaaa
      541 taaaagtaat tcaactgaac ccacaaaatc cttttacatg ggcatcaggt atcaagtcac
      601 ctatatactg tgataacagg ttggttttat catatcctgc catcagaaaa acagccatcg
      661 acggccttga agagttgagc agactcttta gcaacattga tgtaatagct ggtgttgcta
      721 ctgccggtat cccacatggt gcattgcttg cagatcgtct caatcttcct tttatatatg
      781 tacgttcaaa acctaaagaa cacggaaggc aaaatcagat agaaggggcc tacaactccg
      841 gccaacgtgt ggtggtcatt gaagacctga tctctacggg tggtagcagc ttagaggcca
      901 tcgaggcatt acgggcagag ggcttggaag tggtggcttg cctggcattg tttacttacc
      961 agtttgaaaa atcagtaaca agatttgctg aaagtaatgt gcccctccac accgttacag
     1021 actactcgca tatactgaca agagcagtag agcataccta cataacagaa caacagcgag
     1081 ctgttctatc agaatggaaa gataatcctg aaggctggta tgagaaaaat ttcaatagca
     1141 ctacaaatta aattattttt taatatttaa tgactatatg gtaacttttg taataaataa
     1201 tccgttcttt tctgtcaaca accagtgccg tatagcttaa caacgattca aaaacctatg
     1261 tcaataattt caccagtagc cgaaacattt cttgaaaaat atctgaacaa cgcttcacct
     1321 acaggattcg aatatccggg acaaaaacta tggatagaat atatcaaacc ctttatagat
     1381 gaatggcatt cggataatta cggtaccgtt tatggtgtta ttaatccggg tcaggattac
     1441 aaagtagtga tagaagcgca tgcagatgaa atatcttggt ttgtaaatta cataacagat
     1501 gatggcttta tcaatgtaat aaaaaatggt ggttcggatc accttattgc cccttcaaaa
     1561 agagtaaata ttcatacaaa aaacggtttt gtgaaaggtg tattcgggtg gccggctata
     1621 catgtcagaa atggtaaaga accatcccta acacctacca ttgagaatat tttcattgac
     1681 atcggagcga agaataaaga agaagtgctc gaaaaagaca tacatgtagg cacagttatt
     1741 acttttgaag acgaattcat gatgctcaac aataagtatt atgtagggcg tgcacttgac
     1801 aaccgtattg gtggtttctg tattgcagaa gtagcccgca tgcttaaaga taaaaatatc
     1861 aagctccctt atacgcttta tatcgtcaat tcggttcagg aggaaatagg ccttcgcggt
     1921 gctgaaatga tagcacaccg gataaaaccc gacgtggcta tcattacaga cgtaacacac
     1981 gataccaata ctcctctgat gactaagtca aagcttggtg atattaaatc tggcaacggg
     2041 cctaccgtga catacggccc ggctgtacac aacaaattgc tcgaactcat cattggtgca
     2101 gctactgaga acgaaattcc gttccaacga gaagcggcct ccagaagtac aggtacagat
     2161 acggatgctt ttgcttattc gaatgcaggc gtgccgagtg cacttatttc tcttccatta
     2221 agatatatgc acaccacagt agagatggcc aataagtcgg atgtggaaaa tgtaataaaa
     2281 cttatttttg aggctttgca gaaaatagaa agaaaccata attttaatta tttttaaaat
     2341 agccgataaa ttaaaatcat atatttctgc cggttgtcga attttgtcag aaattagacg
     2401 tttttttctt ttggtgttga ctgggtcagt gtttacagag cactatcaaa cttttacttc
     2461 tcggtaattt tctaagtctg agcagccttt tttatgctcc aatttgtttg gcggatttaa
     2521 gtccggtatt ttttccttac ttttgaccaa aagcttttca ttgataaaca tcgaactcgg
     2581 attgctgaaa tattcaccac agttggttac ggtaaaaagc ccggacaaaa tacttgtgcg
     2641 tgacccggta cgaaaaatac tgaccatact gacgcctgag gaaacagtca ggcagttgct
     2701 tattttattt ttgttcgaaa aatattctca cttaaaaaat ctgatcagtg tagaaaaaga
     2761 actcaagatt aattttagaa aaaaacgctt cgatctgctg atatacgacc gccaaatgaa
     2821 accttttttg cttgtggaat gtaaggcgcc gtccattaaa attacacagg cagtctttga
     2881 tcaggtatca tggtataacg ttgccctgaa tgctccgttt ctgctggtca caaatggcat
     2941 ctctacttat tgtgccaaaa tagatttcga caaaaaaact tacaattttt cagattactt
     3001 gccgctggat ttcgaaaatc tctgattgtt ttgctaactt agtgccacca aatttagctt
     3061 aaccaattaa cagcatacat gaaacaagaa aaaattctaa taataggggc aagcggtcag
     3121 ataggtagtg ttttaacaca cacattgtca gatatatacg gagagaataa tgtaatcgct
     3181 actgatatca gacaaccaac agatcaccac ggacatttcg aaatactgga cgttctcaat
     3241 actcaggcac ttggtgatat tgttgccaaa tatggtatta ctcaaatata tcatctggct
     3301 gctatacttt ctgccaaagg cgaaacaatg cctatgtttg cctggcaatt caatatgaca
     3361 agttttttca atgtgataga tgtagcccga cagttcaaga tatcaaaaat atttttcccg
     3421 agcagtatag ccgcatttgg gccttctact cccaaataca atacgcctca ggctactatc
     3481 atgattccgg agacggtcta tggtatctca aaatcttgcg ccgaagattg gtgcaattat
     3541 ttctggctaa aagaaaaaat ggacattcga tcggtacgtt atcccggtat tattggttat
     3601 cagtcgcttc ccggtggtgg tacaacagat tatgccgtaa atatttatca caaagctgta
     3661 aaatcggaac cgttcgaatg cttcctaaaa gctgatactg cattgccaat gatatatatg
     3721 gatgatgcca tccgagccac tatcgaattg atggaagccc cggcagaaaa tataaaaata
     3781 cggacaagct acaacctcgc atctatgagc ttttctccac aggatattgc cgaatcaatt
     3841 aaaaaacaag tgccggactt tagcataagc tacaatccag attttagaca agccattgct
     3901 gaatcatggc ctgcaagcat cgatgattct gaagctagga aagactgggg ctggaggccg
     3961 aattttgacc tcgatgccat gacagccgat atgcttgctc atctttcaga aatgtactca
     4021 aaagaaattg ttcaccacta aagaaaccat caaagctata tgtttaatga agtaagagac
     4081 caacttgcag ctgagcttgc tgccatagaa gagcaaggct tgtataaaag agagagaatc
     4141 atcactacac ctcaggctgc acatatatct accgtagaag gtggcgatgt acttaacttt
     4201 tgtgcgaata attacttggg cctttcatcg caccctgaag tgaccggtgc cgctaagcat
     4261 gcaatagata cgcacggtta tggaatgtct tctgtacgat tcatctgtgg tactcaggac
     4321 atccacaaag agctggagaa aaaaacggca gaatttcttg gcatggaaga ctgtattctg
     4381 tatgcagctg catttgacgc caatggtggc ctattcgaac ccatactcaa tgaagaagat
     4441 gctatcgtat cggacgaact taaccacgca tctatcatcg acggtatccg tctttgcaag
     4501 gctaagcgtt ttcgctacaa aaataatgat atgaacgacc ttgaagccaa attgaaggaa
     4561 gctcagggca gtagaaggat actgatagct acagatggtg ttttctctat ggacggtatc
     4621 gtcgctcaga tagacaaaat atgtgatctt gccgataagt acaatgcgat ggtgatggtt
     4681 gacgaatgtc actctaccgg attcatgggt aaaaccggcc gtggtacaca tgagttgtgc
     4741 aatgccatgg gtagggtaga tatcattacc ggtacatacg gtaaagctct tggaggtgct
     4801 tcaggtgggt ttactgcagc gcgtaaagaa atcgtggaca tgcttcgaca gcgttcgaga
     4861 ccatatcttt tctccaatac actcgctcca tccatcgtag gcggatcgct taaggttctt
     4921 gaattattgt ctgcttccac acatctgcgt gataaactgg agaccaacac tcaacatttc
     4981 agaaaaggaa tgactgaagc cggatttgat attgttccgg gttttcatcc tatcactccg
     5041 gtcatgcttt acgatgccaa actcgctcag gattttgcag caagattgct caaggaaggt
     5101 atttacgtca tagggttctt ttatccggta gtgcctcagg gcaaggctcg tattagggtg
     5161 caggtgtctg caggtcatga gatcgcagat ttagacaaag caatagcagc attcactaaa
     5221 gtgggcaaag aacttggtgt aatataggct ttttctttct tttttgttga aatttgcggt
     5281 accagtcatc cgggtatcgg gaaattggat ttcataagtt gaaatgtata ttttataata
     5341 atataacccc aaatcatgaa atacagcctg acaatattca tcgtattggc ttttacgcta
     5401 ccttcttttg ctcaaccatc accatttatt caggttgacc agtttggtta taaaccattg
     5461 gccgagaaag tagcagtact cagtaacccg attattggat ttaacagcaa tctgtcttac
     5521 caaccacctg caatgatgga agtaaggcgt gctgttacaa atgaaacggt acttgtgggc
     5581 gatgtgaatc tttggaatgg gggcgcagta cacaatcaat caggtgatat gggttggtgg
     5641 ttcgacttca gtggtgtaca agataccggg cattattata tctatgatcc gttaaacaac
     5701 gagtcatcag gaatttttag aattgccgaa aatccatata tcgatgtatt gaaggctgcc
     5761 ggcagaatgt atttctacaa ccgttgtaat gatgaaaaag cactaccatt tgcaggggaa
     5821 aaatggcagg atggtatgaa ttttctccac ccgctacagg atgcaaattg cagatatgtc
     5881 tatgataaaa caaatgctgc actcgaaaaa gaccttcgtg gtggctggtt cgatgctggt
     5941 gactataata aatatgtgtc gttcaccttc gctcctttac atgatttgtt gtttgcctat
     6001 gaagaaaacc ctcaggcatt tggtgatgat tggaatattc ctgaaagcaa gaatggtttt
     6061 ccggacatca tcgatgaagt aaaatgggaa ctcgactggc tgatgcgcat gaccaatgct
     6121 gatggtagtg cctataacaa aatgggcagt atagcttatg atcagaatgc attatcgccg
     6181 ccttctgcca atacagatcc acgttactac ggacctactt gctcctcggc atccataacg
     6241 attgcaagtg ttttgagtca tgcagctgta gtttttaaaa aattcaaagg actggaaggc
     6301 tatgccgttc aattgcagca gacggccgag acctgcttta attactgcct gccgttctac
     6361 ttaaacaaca actgggaaac caattgcgac gatgggtcca tcaattcggg tgattgtgat
     6421 gaaagcgtac aaaatcaatt agaaatattc attaccgctt gtgtttatct ctacgaactc
     6481 acaggtgatg aaacttatca tgattatttt aaggcaaatt acctcaaatc tcgcccaatg
     6541 tccaattcat actggtcggg cgattatcct actatccagg atgcactgat acgttatgcc
     6601 tggaaactcc agaatacgga taagaatgta caactagaca tacagtctga tctgaaagaa
     6661 gcaatagcaa ataactggaa tgatttcttt ggatggaatg acctcgatct ttaccgcgca
     6721 ttcgtgcccg actggacttt cacttgggga ggcaataaac caaacgcatg ttatggtatt
     6781 ctgagtttta atatttctcg ttacaaaatg gtatcagaca cttcaggttt tgtgaagaaa
     6841 ggaaatgaac aactacatta ttttcacggt gtcaatccgc ttggtatggt ttggttgagc
     6901 aatatgtacc cgtatggagc attcaggtgt gcaaaccaga tttatcactc atggttcaag
     6961 gatggtagca tctatgacca tgcgctgaat tcacccaatg gtccggcacc cggatttatg
     7021 gtaggcggac ctaacattta ttacagcatt acaagcatat cgcctccagc caaccaaccg
     7081 gcaatgaaat catacctcga ttttaacaac gactggcctg ataacagttg ggagattacc
     7141 gagccgtcta tttacgtaca ggctgcttac atcaggttgc ttgcctatca cgtcacaaag
     7201 gatattacta caaagattac cgaccagacg acaaacacag catttcatat ttcaccaaat
     7261 cctgcaaacc aatgggtggc tttgtcgggc atcaattttc cggcagatat tgtcattctg
     7321 gatgtacacg gtagaactgt ttttgagaaa tcagacattg ctgcctctga aagtat
//
LOCUS       DAOQQY010000124         7357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4070_length_7357_cov_6.025609, whole genome shotgun sequence.
ACCESSION   DAOQQY010000124 DAOQQY010000000
VERSION     DAOQQY010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_7357_cov_6.025609"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="PLE23_07125"
     CDS             <1..633
                     /locus_tag="PLE23_07125"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HMX83071.1"
                     /translation="IFKILQSYVKKSKKIRFEGDNYSDDWKKEAAKRGLNNLASSPDA
                     LGAYISKSTIDLCKKTGVFSEKELHAHYEAMIDGYNHKMMIESRTLEELCLNYVMPAA
                     SEFQTNLLNNLQGLKNIGQDAKKVASYQVEMVERISTLMNKLHATGKKMSKAREQVLK
                     YTASPERAEAFCQKVKPLMDEIREYCDDLELIVDDRIWPLIKYRELLFMR"
     gene            1367..2227
                     /locus_tag="PLE23_07130"
     CDS             1367..2227
                     /locus_tag="PLE23_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HMX83072.1"
                     /translation="MDITSNKTFTYKELYRFTNRVFLGLGQIILQKNAFAGLLILLAF
                     LVSDITIGLAVLVATVVGTLIARLLNFRNAHTDNGLYGFNAALVGAAMFYFFQPNLLL
                     WIWMPFFSGIATLVYHFFLTRDIPVYTLPFIVIIWVILRFTPVGLLSEQHDSISSAAI
                     MDYYYIIRGFGQIVFQSEILSGLLIFLALVVGSRWAALYTLCACILSGFVALSLGIES
                     DLTNNGLFAFNPILCAVVFAGPGLKNAVNVGIAVVLSMVLSAMMIKAGYVQLTFPFVA
                     SCWITGVLKR"
     gene            complement(2233..2961)
                     /locus_tag="PLE23_07135"
     CDS             complement(2233..2961)
                     /locus_tag="PLE23_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HMX83073.1"
                     /translation="MVLAFFLLSLIFLGVGLTVTESNAKYLVSGYNSLSESERRTIDI
                     KPYIKYFRHFHYFLSGSILILGLLLYYFVSKNLSGIFLGTYPILAYMYFLWRSKVHTI
                     QIGKKEKAKFWAIFSLLCIILLLIGIMFYSSMRENKIITDNNIINITGEYGEKINIRE
                     IKSISLVDSLPDISIKMNGFALETIKKGLFKTQSDETVKLIINSDQHPLLLITTEDGR
                     KIYYSAKTNSNKQLYDELRMRLNK"
     gene            complement(2971..3951)
                     /locus_tag="PLE23_07140"
     CDS             complement(2971..3951)
                     /locus_tag="PLE23_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMX83074.1"
                     /translation="MKYCNNILETIGNTPLVKINKITADIPALVLGKIETFNPGHSIK
                     DRMAVKMIDDAEAKGLLKPGGTIIECTSGNTGMGIALVACVRGYKTIFTTSDKQSKEK
                     FDVLRALGAEVIVCPTAVEPDDPRSYYSVAKKLSEETPNSFWANQYDNLSNREAHYLS
                     TGPEIWEQTDGKITHLVVGVGTGGTVSGTARYLKERNPNIKVWGIDTYGSVFKKYKET
                     GIFDPNEIHSYITEGIGEDILPKNVDFDLIDAFEKVTDKDGALMARRLAREEGLLLGY
                     SAGSAMAGVLQLKENFSKDDVVVVIFHDHGSRYVGKIYNDDWMRANGFIE"
     gene            complement(3958..4821)
                     /locus_tag="PLE23_07145"
     CDS             complement(3958..4821)
                     /locus_tag="PLE23_07145"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMX83075.1"
                     /translation="MNRIIHSFIICFVVLCKISAQTTGDSTRYLLPHDSLFISLDEFP
                     EKIIRHTMSKKQTLFGLARHYGLQVDELAYFNPDLNLNEVKIGDQIKVPIPNASIKRF
                     KQKGFLRWKYAPVYYRVKTGDTMFKIAKSYFKMPVDTLKKRNKYWKEEVQLGQVLQVA
                     WLSTKGVPDSLQHFKKHPLWKVSDEMEAKFITDSKDKKIKTQKGVTVWPKGIANTSSD
                     SYILHRTAKIGSVILITNPMNNRKVYVKCIGRLPERVYGNEVLAIVSTPVANLLGVID
                     ERFFSTLKYIK"
     gene            4904..5647
                     /gene="kdsB"
                     /locus_tag="PLE23_07150"
     CDS             4904..5647
                     /gene="kdsB"
                     /locus_tag="PLE23_07150"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HMX83076.1"
                     /translation="MSNQKLKKIGIIPARYASTRFPGKPLVQIHGKSMIQRVYEQAIK
                     SELDSVIVATDDLRIFDHVADFEGNVLYTAGTHLTGTDRCYEVAANLPFDCIIINIQG
                     DEPFINPEQINELLRVFDRKKSITIATQCKLITSNELIFDANVVKVVKNTKGFAMYFS
                     RNPIPYVRNARQEDWVQHQKHYKHIGIYGFRKSVLKKLAALPSGVLESAESLEQLRWL
                     ENGHRIFVAETNYETFGIDVPEDLDKIVF"
     gene            complement(5657..6136)
                     /locus_tag="PLE23_07155"
     CDS             complement(5657..6136)
                     /locus_tag="PLE23_07155"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HMX83077.1"
                     /translation="MLLKIETEYYIYDSLEDLSEIQRSLLLRAKQNTHHAYAPYSKFY
                     VGAAILTDDGKVIDGSNHENAAYPMCLCAEQVAIANFHQHRTDQKIKSIAISARTADK
                     LIDSPVPPCGACRQILLEVAEKLKADYEVILQGETGKIFVIPSVKALLPIQFDSSNL"
     gene            6233..>7357
                     /locus_tag="PLE23_07160"
     CDS             6233..>7357
                     /locus_tag="PLE23_07160"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMX83078.1"
                     /translation="MKILLFGAGRSALFFVEYILHHSTTFNWKLSIADFNFNNIDEKL
                     KQHPNLSLVQSDINDVAKRETLITAHDMVVSLLPAHLHILIAKDCLTFNKPFANASYV
                     TDELRSLEPELLKKNLLFAGELGLDPGIDHMSAMETIHTLQAKGAHITSFTSYTGGLI
                     APESDDNPWHYKISWNPRNIILAGQGIAQFKKEGRVVYVPYHQLFRNTELLNIAPYGK
                     YEGYPNRDSLHYEELYGLGGIETLLRGTIRANGFCVAWNILIQCGYTNDTIKIDTDRF
                     TLKEITRAFIQDEYDSLTSSKIEKLLGIKVSPKSVETLRWLRLDSNVLLNKGMLTPAQ
                     VLEYWLIDKWRLADDDKDMVIMQHKYQYTLDGIKYSMTSTL"
BASE COUNT         2223 a         1381 c         1386 g         2367 t
ORIGIN      
        1 atattcaaga tattacagag ttatgttaaa aagtcgaaga aaatcaggtt tgaaggtgat
       61 aattacagtg atgactggaa aaaagaagct gctaaacgag gtttaaataa cctggctagc
      121 agtccggatg cactcggcgc ttatatttcg aaatcaacaa tcgatctttg taaaaaaaca
      181 ggggtgttct cagaaaaaga actccatgca cattatgaag ccatgattga cggctacaat
      241 cacaaaatga tgattgaatc aagaaccttg gaagaactat gccttaatta tgtaatgccg
      301 gcagcatcag aattccagac taatctactt aataatctac aaggtttgaa aaatattggt
      361 caggatgcga agaaggtggc ctcttatcag gtagaaatgg tagaacgcat cagtacactc
      421 atgaataaac tacacgccac cggtaaaaag atgtcaaaag ctcgtgagca ggtattgaaa
      481 tatacagcaa gcccggagag ggcagaggcg ttctgccaaa aagtcaaacc tctcatggat
      541 gaaatcagag aatactgtga tgatctcgag ttaattgtag atgacagaat atggccgttg
      601 attaaatacc gcgaattgtt gtttatgaga taatttttca agaaacataa ttcaaccaac
      661 taaaggcatt ttgagcaatc aggatgcctt ttttgttaaa attcgttaaa attttataaa
      721 aataattgga aataagtcgt aaaagattaa aattattatt cacaaatatc aaagtatatt
      781 gaaatatatc aatttatata tgattgataa tcattagtat agtaaatatt aattgactaa
      841 tataatataa tttattttca caatagtatg tttttgtttt tgttttgcct aaaaagaagg
      901 tgaatatttg tatcataatt actgagttaa cgaactcgga cacgaattca gaaatttttc
      961 atgggaaata taatgtgtgg cggtgggtcg ccacttgcat aaagaagcga ctcacctgtt
     1021 ccaattttct ggattccaat taacttcgga tatgttcatg ggattatatg atgtgtggcg
     1081 gtgagtcgcc acttcaaaag aagcgactca ccaaacactt ttttctgatc tcatcttaaa
     1141 taaggatatg ttcatgggat tataatttgt tgttaagtgc cgctatcacg cggcactttt
     1201 ttaaaagcct cctctacaca ggagcacatt gacttcggat atgttcatgg gattatattt
     1261 tttcaaagag ccgcttatca tgcggctttt tttttaattt taatatattc gagtaacaat
     1321 tacttttaga acatatactc tacacaatca tctatttaaa gatataatgg atattacttc
     1381 caataaaaca tttacataca aagaattata caggtttacg aatcgagttt ttcttgggtt
     1441 aggacagatc attcttcaga aaaacgcatt tgccggactc ttaatattgt tggcattttt
     1501 agtttctgat attactatag gtttggctgt tttggtggcg accgtagttg gcacactcat
     1561 cgcccgattg cttaatttta gaaacgctca taccgataat ggtttgtatg gtttcaatgc
     1621 agcacttgtg ggtgccgcaa tgttttactt ttttcaaccc aaccttttac tatggatttg
     1681 gatgcccttt ttttccggca ttgctacact ggtatatcat tttttcctta caagagatat
     1741 tccggtttat actttaccat ttattgtaat catctgggta atacttcggt ttacacctgt
     1801 agggctttta tctgaacaac atgactccat ctcttcagct gccattatgg attactatta
     1861 cattattcgc ggatttggtc agatagtttt tcagagtgaa attttatcag gcttattgat
     1921 ttttcttgca ctcgtggtcg gttctcgttg ggcagcatta tacacattgt gcgcttgtat
     1981 tttatcaggt tttgttgcct tatcgcttgg aatagaatcc gatttaacca acaatggctt
     2041 gtttgctttt aatccgatat tgtgtgctgt tgtatttgcc ggacctggcc tcaagaatgc
     2101 tgtcaatgta ggaattgctg ttgtactctc aatggtgttg agtgcaatga tgatcaaggc
     2161 aggatatgtc caacttacat ttccttttgt ggcttcatgc tggatcactg gtgtgctaaa
     2221 gcgttgaaca ttttacttgt tcaatctcat ccttaattcg tcgtataact gtttattact
     2281 gttagttttg gcagaataat atatttttct accatcctcg gtagtgatga gtaacagagg
     2341 atgttggtca gaattgatta tgagtttaac cgtttcgtct gattgtgttt taaaaagtcc
     2401 tttcttgatt gtttctaatg caaagccatt cattttaata cttatatcag gtaaagaatc
     2461 tacaagtgaa atggatttta tctcccggat attaattttt tcaccatatt cacctgtaat
     2521 gtttatgatg ttgttgtctg taataatttt attttcacgc atactactat aaaacataat
     2581 accgattaat agtagtatga tacagagtaa cgaaaatatt gcccaaaatt tggccttctc
     2641 ttttttacca atctgaatag tatgtacttt gcttcgccac agaaaataca tgtaggcaag
     2701 aatggggtat gtgccaagaa atataccact caaattctta ctcacaaaat aatacaaaag
     2761 tagcccaagt atcagaattg aaccggaaag aaagtaatgg aaatgtcgaa aatacttaat
     2821 atatggcttg atgtcaatag tacggcgttc actttcagat agtgaattat aaccagatac
     2881 caaatatttt gcatttgatt cagtaaccgt caacccaaca cccagaaaaa tcaggctcag
     2941 gagaaaaaaa gcaagtacca tggttgaaaa ttactcaata aaaccattag ccctcatcca
     3001 gtcatcgttg tatatttttc ctacataacg cgagccatga tcatggaata ttacaacaac
     3061 cacatcatct ttgctgaaat tttcctttaa ttgcaataca cctgccatag ctgagccagc
     3121 tgaatatccg agtaacagac cttcttcacg agcaagacgt cttgccatta gagcaccatc
     3181 cttgtccgtt accttttcga aagcgtcaat caggtcaaag tcaacatttt taggcagtat
     3241 atcttcaccg ataccttccg taatgtaaga gtggatttca tttgggtcga aaatgcctgt
     3301 ttctttgtat ttcttaaata cagagccgta ggtgtctatg ccccagactt tgatgttcgg
     3361 attcctttct ttgaggtatc tggctgtacc actgacagta cctcccgtac caacacctac
     3421 tacaagatgt gttatcttgc catctgtttg ttcccatatt tcaggtcctg ttgacagata
     3481 atgtgcttcg cggttcgaga gattgtcgta ttgatttgcc caaaacgaat tgggcgtttc
     3541 ttcacttagt ttttttgcta cagaatagta cgaccttggg tcatcaggtt ctactgcagt
     3601 aggacacact atgacttcgg cacctaatgc tcttagtaca tcaaattttt cctttgactg
     3661 tttgtcactt gtagtgaaaa tggttttata accacgtaca caagccacca atgcgatacc
     3721 catacctgta ttgccagagg tacactcaat gatggtacca ccgggtttga gcaatccttt
     3781 tgcttctgcg tcatcaatca tttttacagc cattcgatct ttaattgaat gacccgggtt
     3841 aaatgtttcg attttgccca ataccaaagc aggtatatca gcagttattt tattgatttt
     3901 taccaacggt gtattgccga tggtttccag gatgttgtta caatatttca aaatctgtta
     3961 ttttatgtat tttaatgttg aaaaaaatct ttcgtctatt acgcccaaca aattggctac
     4021 aggcgtagat actattgcta acacttcatt tccataaaca cgttcaggaa gtcggccaat
     4081 acattttaca taaacttttc tattattcat tggattggtg attaatataa ctgaaccgat
     4141 tttggcggtt cggtgcagaa tataactgtc ggatgatgtg tttgcaatcc cttttggcca
     4201 gactgtaact cctttttgtg tttttatctt tttatccttt gaatctgtga tgaatttcgc
     4261 ttccatttca tcagaaacct tccacaacgg atgctttttg aaatgctgta ggctgtcggg
     4321 tactcctttg gttgataacc atgccacttg taatacctga cctagttgaa cttcctcttt
     4381 ccagtattta ttgcgcttct tgagcgtgtc cacaggcatc ttgaaatacg actttgcaat
     4441 cttaaacatc gtgtcaccag tctttactct gtaatacaca ggagcatact tccaacggag
     4501 gaatcctttt tgcttaaaac gcttaatgga agcattgggt atgggtactt ttatttggtc
     4561 gccgatcttc acttcattca gattgagatc agggttaaaa tatgcaagtt catctacctg
     4621 cagtccataa tggcgtgcca gaccgaacaa ggtctgtttt ttagacattg tatgccggat
     4681 aattttctcc ggaaattcgt caagtgagat gaaaagcgaa tcatgtggca ataaataacg
     4741 ggtactgtca cctgtagttt gggcagatat tttacacaat actacaaaac atataataaa
     4801 gctatgtata attctgttca tgagtgataa ctttgcaggc acaaatgtaa tcaattgtat
     4861 aaattacaca taatcaatcg ttcaaatatt atagattact aagatgtcaa accaaaaatt
     4921 aaaaaaaata ggtatcattc cggcacgcta tgcttctacg cgttttccgg gtaaaccttt
     4981 ggttcagatc catggcaagt caatgattca gagagtttac gaacaggcca ttaaatcaga
     5041 actcgactca gtgatagtgg caacggatga cctgcgtatt tttgatcatg tggctgattt
     5101 tgagggcaat gtattatata ctgctggtac ccacctcact ggtaccgacc gttgttatga
     5161 agtggcagca aacttacctt tcgattgcat catcatcaat atacagggag acgaaccttt
     5221 tataaaccct gaacagatta atgagttgct gcgtgtcttt gatcgaaaaa agagtattac
     5281 cattgctacc caatgcaaac ttataactag taatgaactt atttttgatg caaacgtggt
     5341 aaaagtagta aaaaatacaa aaggcttcgc aatgtatttt tcacgtaatc ccatacctta
     5401 tgttcgcaat gcccgacagg aagattgggt acagcaccaa aaacattaca aacatattgg
     5461 tatctatggg tttagaaagt cagtccttaa aaaattggca gctctaccat ccggagtact
     5521 cgaatcggcc gaaagccttg aacaactccg atggttagaa aacggacacc gcatctttgt
     5581 agctgagacc aactacgaaa ccttcggaat agatgtaccg gaagatcttg ataaaatagt
     5641 tttttagata attccttcaa agattactgc tgtcaaactg aattggcaat aatgccttta
     5701 ccgagggaat gacaaaaatt tttccggttt ctccttggag aatgacttca taatcggcct
     5761 ttaatttttc ggctacttcc aacaatatct gtcggcaagc accacatgga ggtaccggac
     5821 tgtcaatcaa tttgtctgca gtacgcgctg agatggcaat tgatttgatt ttctgatcgg
     5881 ttctgtgttg gtgaaaattg gctattgcaa cctgttcggc acacagacac atcggataag
     5941 ccgcattctc gtgatttgat ccgtcgatca ccttgccatc atctgttagt atagcagctc
     6001 ctacatagaa ttttgagtaa ggtgcatacg catggtgcgt gttttgcttg gcacgtagca
     6061 ataaacttct ttgtatttcg ctcaaatctt ctaaactatc gtaaatgtaa tattcagttt
     6121 cgattttcaa taacataatc atcaaatttt ttgattcaaa tttgcctcgt taggtctgat
     6181 tattacgata aaaatggtag gtttgttgtg tcaattaata aaaaggctaa atatgaagat
     6241 tctgttgttt ggtgcgggga ggtcggcatt gttttttgtg gaatatattc ttcatcatag
     6301 tactacattc aactggaaac tcagcattgc tgattttaat tttaataaca ttgatgaaaa
     6361 attgaagcag catcccaatc tgagtctggt tcaatcggat ataaatgatg tagcaaaaag
     6421 agagactctg attacagcac atgatatggt tgtctcatta ttaccggccc accttcacat
     6481 cctcattgcc aaagattgtc ttacatttaa taaaccattc gcaaatgctt cctacgtcac
     6541 agacgagtta agaagtcttg aacccgaatt gttaaagaaa aatctgctat ttgccggcga
     6601 acttggtctt gacccgggta ttgaccacat gtctgcaatg gaaactattc acacgcttca
     6661 ggcaaaaggt gcacacatta catctttcac ctcctatacc ggtgggctca tagctcccga
     6721 gtcggatgat aatccttggc attacaaaat tagctggaac ccaaggaata ttatattggc
     6781 aggacaagga atagcacagt ttaaaaaaga gggtcgggta gtgtatgtac cttatcacca
     6841 attgttcagg aataccgagt tgttgaatat tgctccttat ggtaaatatg aaggctaccc
     6901 aaaccgtgac tcattgcatt acgaagaatt gtacggactt ggcggaattg aaacattgtt
     6961 aagagggacc atccgtgcca acggattctg cgtagcctgg aatatactaa tacaatgtgg
     7021 atacaccaat gatacaataa aaatagatac agacagattt acgcttaaag aaataacaag
     7081 ggcttttata caggatgaat acgactcgct cacatccagc aaaatcgaga aactgcttgg
     7141 aataaaagtt tcaccaaaat ctgttgaaac gctcaggtgg ctccgtcttg acagtaatgt
     7201 tttgttgaat aaaggaatgc tgacaccggc acaggtactc gaatactggc ttatcgacaa
     7261 atggaggcta gctgatgatg ataaagatat ggtcatcatg caacacaaat atcaatacac
     7321 acttgatggc ataaaatact caatgacgag tacattg
//
LOCUS       DAOQQY010000125         7357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4071_length_7357_cov_4.911668, whole genome shotgun sequence.
ACCESSION   DAOQQY010000125 DAOQQY010000000
VERSION     DAOQQY010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7357
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4071_length_7357_cov_4.911668"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="PLE23_07165"
     CDS             complement(<1..978)
                     /locus_tag="PLE23_07165"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HMX83079.1"
                     /translation="MNRAIVWFRQDLRLHDNEAITEALKFADEVLYVYIFEDTFLKSK
                     SRFGFQRTEKHRLKFIIESVADLRENLRASNADLIVRCGNAEDELFKIAARYKSAKIF
                     CNRESTHDEILIQDKLEKRLWTIGQEIHYVRGKMLYYTQDLPFPISHTPDSFKAYRKE
                     VEKIVPVRKPLETPLDFKYQSDHIDAGRMPELSDYGHDAALKLPYSAYPFKGGETEGR
                     KRMEYFLRQSNLMATYSESRLGLSGSDYSTKLSAYLAQGCISPKYFYHQITDYETIHG
                     ESKSTYELKLDLLWRDYLRLIAKKYKTMIFYKNCLKSAEKSITNYDTTAI"
     gene            1061..1933
                     /locus_tag="PLE23_07170"
     CDS             1061..1933
                     /locus_tag="PLE23_07170"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HMX83080.1"
                     /translation="MSNILRLLLFSLFSLCMSSMYSQSPAAYKIYSGNGKETDWDELV
                     KKASKSDVVFFGELHNNAVAHWLQLVLAKELYNKSEHRIALGFEMFESDNQRSIDEYF
                     ADIISQKNFEAEVRLWPNYKTDYKPIVEWAKTNKLRVLATNIPRRYANLVYRNGLEGL
                     NMLSDEAKKEIAPLPIAFDINLPGYNAMVDNSTPGHANENLPKAQAIKDATMAYFISK
                     NLIPKGIFLHLNGAYHSNNKEGILWYLARLNPGIQQFTITTVSQDNISELEEEYLNQA
                     DFIIVVPSDFTNTH"
     gene            1949..2362
                     /locus_tag="PLE23_07175"
     CDS             1949..2362
                     /locus_tag="PLE23_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83081.1"
                     /translation="MKHLFYLLALILFVASCKNVTGDQEKQLFDDVMKLHDEIMPVST
                     KLQGLQSSLKAILEKPDSIAGGLTQDDIIQASMHAEKAYNDMMDWMRDFKIPENMNSQ
                     DKIAYLTAEKNKLVKMKEESLSALHEAEAILKTKK"
     gene            2362..2964
                     /locus_tag="PLE23_07180"
     CDS             2362..2964
                     /locus_tag="PLE23_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMX83082.1"
                     /translation="MRFLLLIFALGIFACEYTPAKLPILGERDFVNGDTVYHTVRPFS
                     FIDQDSQQVTEKTFGGKVYVVDFFFTHCPTICPKVTKQMLRLYEKYKDDSRVLLLSHS
                     IDTRNDTVGRLQEYARNLDVSSKSWHFVTGNSDSIYSIAADYFSFAKEDAESPGGFVH
                     SGQLILADKHRRIRAFCDGTDEKDVTRFMKEIDVLLNEKE"
     gene            2951..3349
                     /locus_tag="PLE23_07185"
     CDS             2951..3349
                     /locus_tag="PLE23_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMX83083.1"
                     /translation="MKKNSLIILASIIFTIIVSCKQETYFQGRLLYEDYCQNCHMEDG
                     TGLRGIIPPLKGSDFLKNNQNALVCLIRKGSQGGLTINGSKYTQPMPGNANLTQGDIA
                     NIINYINHAWDNNYGVILYDDVLKQFETCR"
     gene            complement(3370..4758)
                     /locus_tag="PLE23_07190"
     CDS             complement(3370..4758)
                     /locus_tag="PLE23_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMX83084.1"
                     /translation="MRYVKLKFLLLSFETVILKLRATYSQIFKLSVPIMIGSAAQNVV
                     ALSDSVFLYHLSELDFAAIGFVGVFYLVIAGIGFGFTRGGQLMIARRMGEGRMREIGR
                     TFYSMFYFELLMATVMFLFLTFGAEAFFRLFIHSELILQKSLEYLHYRSFGLFFSYTG
                     VAIIALYAGVARTTFIVIDTIILTVLNLVLNYALVFGELGFPEMGIGGSALASTLAEI
                     IAFIAFIIYILFDKKARTYRLFKIPNIDIQLVKQQLSISLPVVGQTVLALGSWFVFIS
                     LIESIGERPLAISNLIRIAYLILMIPCWGFSSGIHTLVSNVIGQKKIEEVIPLAFRTS
                     VLSFVVTMCIAIPITVWPEVFLYPLLGSTNMSLIEESRPLFMILLGIISIYSVSTIFF
                     NTIGGTGATDFTLKIQTVMTILYLMVVFLLVKYGNGGVEWAWAVEIFYWVVILVVSIW
                     YLRTPRWHHYKV"
     gene            4791..6830
                     /locus_tag="PLE23_07195"
     CDS             4791..6830
                     /locus_tag="PLE23_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMX83085.1"
                     /translation="MTYPLARKKEKTLSIHGYTRHDPYYWLNDRENAEVIDYLKAENE
                     YTEYKFEKIKPLKEKLYNEITGRIKQNDSSVPFKWRTHYYQTIYKEGKEYPIYIQAPT
                     QDMNGHTVLLDANIEAEGKSYYHVAGFEVTPGNQILAYAEDTLSRRIYTIRFKNLVTS
                     DYYSWAIPQTSGNMIWSNDGKYLFFVEIDQTTLRPWRVKRWSMENPTNEAVTVFTEND
                     ETYYVSISTAMSGRYLFINSSSTLTTEEQIIDLDNPEQMPITFLPRKSGHEYRIEHAE
                     DFFYIHTNEDGRNFCLKRVAQNDFAHQNWDVVVPHDEDSLLEDFTVINGHLIYDLRKN
                     ANHHIVIRNDAQTYDIPFEEGAYLAFIGTNADPESKFVRINYTSLTTPTSVMDFDFYT
                     REMTLRKQEEITGGYDKTSYTSKRLWVTGRDGVKIPVSLVYNKHLYKPNQSPLLLYGY
                     GSYGHSIDPSFGLARLSLLDRGFVYAIAHIRGGEEMGRAWYENGRQLAKMNTFYDFID
                     CGKELISQGYCHPEKLYAMGGSAGGLLMGAVVNLEPQLWAGIVAAVPFVDVLTTMLDD
                     SIPLTTGEYDEWGNPNIKEYYEYMAQYSPYDNIQPGIYPPMLVTTGLHDSQVQYWEPA
                     KWVAKIRDIVHDSHLIFLNTNMDAGHGGASGRFRRYDEIALQYAFLIGLNEGILS"
     gene            6903..>7357
                     /locus_tag="PLE23_07200"
     CDS             6903..>7357
                     /locus_tag="PLE23_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83086.1"
                     /translation="MFVKLKFWFSIFFAILFLANQLSAVWVGLSLERHKLQFQASLGK
                     NLLSFSEEYLAKNATFRWIKPGIEFTVNHQMYDVKHTEMVKGVNVFHCKHDVHESKIL
                     KRLKKNLKEDSGKAPKGNANTVKVSIATLFCQSLPIFQYLIFDQYKTVI"
BASE COUNT         2399 a         1509 c         1430 g         2019 t
ORIGIN      
        1 gattgcagtc gtatcatagt ttgtaattga tttttccgca gacttcagac aatttttgta
       61 aaatatcatt gtcttgtact ttttagcaat tagcctaagg taatcacgcc acaataggtc
      121 cagtttcaat tcgtaggttg atttgctttc accatggatt gtttcgtagt cagttatttg
      181 gtggtaaaag tactttggcg aaatacaacc ttgagcaaga taggcagata atttggtact
      241 gtagtctgag ccggaaagtc caagccttga ttcgctgtag gtagccatca gatttgattg
      301 cctgagaaaa tactccatcc ttttccgacc ttcggtttcg ccacctttaa agggataagc
      361 cgaatatggc aacttcaatg ccgcatcatg tccgtaatcg cttaattccg gcattcttcc
      421 agcgtcaatg tggtcggact gatatttgaa atcaagaggt gtttcaagag gtttacgcac
      481 cggaacaatc ttttcaactt cttttcggta tgctttaaaa ctatctggtg tatgcgaaat
      541 gggaaaaggc aaatcctgtg tgtagtacag cattttccct cgtacataat ggatttcttg
      601 ccctattgtc cataagcgtt tttcgagctt gtcctgtatg agtatttcat catgcgtact
      661 ttctctgttg cagaatattt tggccgactt atacctggcg gctattttaa ataattcgtc
      721 ttcagcattt ccacatctga caatgaggtc ggcattagaa gcacggaggt tttcacgcaa
      781 gtcggcaaca gactcaatga tgaactttaa tcggtgtttc tcagtacgtt gaaatccaaa
      841 tctactctta gactttaaaa aagtatcttc gaatatatat acatacaaga cctcatcagc
      901 aaattttaat gcttcggtga ttgcttcatt atcatgcaac ctcaggtcct gtctgaacca
      961 cacaatcgcc ctattcaaaa ttatttttat tattcaaaca aatgagacag ttgaattgtt
     1021 tattaatttt tacttttgta gctaaacaca tattacctac atgtcaaata ttttgaggct
     1081 ccttttgttc tcgttgttca gtttatgtat gtcatccatg tatagccagt cacccgcagc
     1141 atataagatt tattccggta acggaaaaga aacagattgg gacgaacttg taaaaaaggc
     1201 ttcgaaatcg gacgtagttt tcttcggtga gctgcacaac aacgctgttg cgcattggct
     1261 gcagttggtg ctggcaaaag aattgtataa taaatcagaa cacaggattg ctttaggttt
     1321 tgaaatgttt gagtctgaca atcagaggag tatcgatgaa tatttcgctg acattatttc
     1381 gcaaaagaac tttgaagcag aggtcaggct ttggccaaat tacaaaacag actacaaacc
     1441 cattgtagaa tgggccaaga ccaataaatt gcgtgtatta gcaaccaaca taccaagacg
     1501 ctatgccaat ctggtttacc gaaatggcct tgaaggctta aatatgttaa gcgatgaggc
     1561 aaaaaaagaa attgcaccgc ttccaatagc tttcgatatc aatctgccgg gttacaatgc
     1621 tatggtggat aatagcacac cgggtcatgc caatgaaaat ctacccaaag cccaagcaat
     1681 aaaagatgct accatggcat attttatttc aaaaaacctg attcccaaag gcatattcct
     1741 gcatctcaac ggagcttacc attctaacaa caaagaagga atactttggt atcttgcaag
     1801 gttaaatccc ggaatacaac aatttaccat taccaccgtt tcgcaggaca acatcagtga
     1861 actcgaagag gaatatctta accaagcaga ttttatcatt gtagtacctt ctgattttac
     1921 caacactcat taaaaacaat aaaatataat gaaacattta ttttacctat tggcactcat
     1981 tctctttgtt gcttcatgta aaaatgtaac cggcgaccag gaaaaacagc ttttcgatga
     2041 tgtgatgaaa cttcacgacg agattatgcc ggtttcaacc aaattgcagg gtttgcaaag
     2101 ttcattaaaa gccatactcg aaaaacccga ttcgatagca ggcggattaa ctcaggacga
     2161 catcatccag gcttctatgc atgctgaaaa agcatacaat gacatgatgg actggatgcg
     2221 tgacttcaag attccggaga acatgaactc acaggataaa attgcatatc ttacagctga
     2281 aaaaaacaag cttgttaaaa tgaaagaaga atctctgagt gctttacatg aagcagaggc
     2341 aatcttaaaa accaaaaaat aatgcgcttt ttgttattaa tattcgcact tggcatattt
     2401 gcctgtgaat atacacctgc caaattacct atattgggcg aacgtgactt cgtcaatgga
     2461 gatactgtct atcatactgt cagaccattc agttttatag accaggactc acaacaagtg
     2521 actgaaaaga cattcggagg caaagtatat gtggtagatt tctttttcac ccactgccct
     2581 accatctgcc ctaaagtaac caaacaaatg cttaggttgt atgaaaagta taaagatgac
     2641 agccgcgtac ttttgttgtc acacagcatt gacacgcgta atgatacagt aggccgactg
     2701 caggaatatg cccggaacct cgatgtaagc agcaaaagct ggcatttcgt aacaggcaat
     2761 agtgattcca tttacagtat tgcagccgac tactttagct ttgccaagga ggatgctgaa
     2821 agcccgggcg gattcgtaca tagtggccag ctcatactag cagataagca caggcgtatc
     2881 agggcatttt gcgatggcac ggatgagaaa gacgtcacac gttttatgaa ggaaatagat
     2941 gtgttgctca atgaaaaaga atagcctcat tatattagca tccatcattt ttactataat
     3001 agtatcttgt aagcaggaaa catattttca aggaaggttg ttgtacgaag attattgcca
     3061 gaattgccac atggaagacg gcaccggact aagaggcatc ataccgccac tgaaaggatc
     3121 cgattttttg aaaaataatc agaatgcgct cgtatgcctc attcgtaaag gaagccaagg
     3181 cggcctgacc atcaacggca gtaaatatac acaacccatg cccggcaatg cgaacctaac
     3241 ccaaggtgat atagccaata ttatcaacta catcaaccat gcttgggaca acaactatgg
     3301 ggtcatacta tacgacgatg ttttgaagca atttgaaact tgccgataaa tacttatttt
     3361 gttaaaaaat tataccttgt agtggtgcca acgtggtgtg cgcaagtacc atatacttac
     3421 taccagaatt actacccaat agaatatttc aactgcccac gcccattcga ccccaccatt
     3481 accatatttc actaaaagga atacaaccat caggtacaga atcgtcatca ctgtctgtat
     3541 ttttaaagta aaatctgtag caccggtacc tccaatggta ttaaagaaaa tagtgctaac
     3601 tgaatatatg ctgatgatgc cgagcaatat catgaatagt ggccttgact cttcaatgag
     3661 actcatgtta gtagaaccca ggagtgggta aagaaaaacc tcaggccaca cggtaatagg
     3721 tatagctata cacatggtca ctacaaatga aagaacagaa gtacgaaagg ccagcggaat
     3781 tacttcttca atcttttttt gcccaatgac attactcacc agcgtgtgta tgccacttga
     3841 aaaaccccag cacggtatca ttagtattag gtaggcaatc cgtataaggt tggaaatagc
     3901 gagcggtctc tctcctattg attctatgag agatatgaac acaaaccacg aacctagagc
     3961 aagcacagtt tgccctacta caggtaatga aatacttaac tgctgcttta caagttggat
     4021 atcgatgttt ggtatcttga aaagccggta tgtccgagcc tttttatcaa aaagtatata
     4081 aataataaat gcgataaatg caataatttc agcaagtgta ctagccagtg cagaaccacc
     4141 gatacccatt tcgggaaaac ccagttcacc aaatactagg gcataattga gtaccagatt
     4201 aagcacggta agtatgatcg tgtctataac aatgaatgtt gtacgagcca caccggcata
     4261 tagcgcaatg attgctacac cagtataact gaaaaacaag ccaaatgacc tgtaatgcaa
     4321 gtattccagg cttttctgca atatcaattc cgaatgtatg aacaacctga aaaaggcttc
     4381 tgctccgaaa gtcaggaaaa gaaacataac tgtagccatc aaaagctcaa agtaaaacat
     4441 agagtagaag gtgcgaccta tttcgcgcat ccttccctcg cccattcttc tggctatcat
     4501 aagctgaccg ccacgtgtaa acccaaaccc gatgccggct atcaccagat aaaatacgcc
     4561 cacaaaacca atagccgcaa aatcaagctc gcttaggtga tacaaaaaaa cactgtcact
     4621 taaggctaca acattctgtg cagcactacc aatcataatc ggtaccgaaa gcttaaaaat
     4681 ctgagaatat gtggctctta gtttcaaaat aactgtttca aaactcaata ataagaattt
     4741 taattttaca tatctcattc tttcttgata tttgcgaaaa aataattcaa atgacttacc
     4801 cactggcaag aaaaaaagaa aaaacactct ccatccacgg ctatacaaga catgacccat
     4861 attattggct taatgacaga gaaaatgctg aagtaatcga ttatctgaaa gcagaaaatg
     4921 aatatactga atacaaattc gagaaaatca agccactaaa agaaaaatta tataatgaaa
     4981 taaccggacg gatcaagcaa aatgactcat ctgtgccttt caagtggcgt acacactact
     5041 accagaccat ttacaaagaa ggcaaagaat atcccattta catacaggcc cccacacagg
     5101 acatgaatgg tcataccgta ctacttgatg ccaatattga agcggaagga aaatcatact
     5161 atcatgtggc aggtttcgaa gtcaccccgg gcaatcaaat tctcgcctat gcagaagata
     5221 cgctcagccg tcgcatttat accatccgtt tcaagaacct ggtgactagt gactattaca
     5281 gctgggcaat accgcaaaca tcaggtaata tgatatggtc aaatgacgga aaatatctct
     5341 tttttgtgga aatagaccaa acaaccttac gaccgtggag agtaaaaaga tggtcaatgg
     5401 aaaatccaac caacgaagcg gtaactgtat tcacagaaaa tgacgaaact tactatgtaa
     5461 gcatctcaac tgccatgagc ggacggtatc ttttcattaa cagcagctct acacttacta
     5521 ctgaagaaca gattatagac cttgacaatc ctgaacaaat gccgattaca tttctgccta
     5581 gaaagtccgg acacgaatac aggattgaac atgcagaaga ttttttctac attcacacaa
     5641 atgaagatgg ccggaatttt tgtttaaaac gggtagccca aaatgatttt gcacatcaaa
     5701 actgggatgt agtcgttccg catgatgaag acagccttct cgaagatttc accgttatca
     5761 atggacattt aatatatgat ctgagaaaaa atgcaaacca ccacattgtc atacgcaatg
     5821 atgcccagac ttacgatatt ccatttgaag aaggtgctta ccttgcattt attggtacca
     5881 atgccgatcc tgaaagtaag tttgtacgga tcaattacac ttcactcacc acaccaacga
     5941 gtgttatgga tttcgatttt tatacaagag aaatgacatt gcggaagcag gaagagataa
     6001 ccggtggata cgacaaaacg tcctacacat caaaaagact ttgggtcacc ggtcgtgacg
     6061 gggtaaaaat acctgtaagc ctcgtttata acaaacattt atacaaacca aaccaatcac
     6121 ctttactatt gtacggctac ggctcgtatg ggcacagcat agatccttca tttgggcttg
     6181 cccgtttaag tttgcttgac cgcggctttg tgtatgccat tgctcatata cgcggaggag
     6241 aagaaatggg cagagcctgg tatgaaaacg gaagacaact cgccaagatg aatacttttt
     6301 acgactttat tgattgcgga aaagaactta tcagccaggg ttattgtcac ccggaaaaac
     6361 tctatgcaat gggtggaagt gccggtggcc tcctgatggg agccgttgtt aatcttgaac
     6421 cgcagctttg ggccgggata gtggcagccg taccctttgt ggatgtactc actactatgc
     6481 tagatgacag cattccgctc actaccggcg aatatgatga atggggcaac ccgaacatta
     6541 aagaatatta tgaatacatg gcacaatatt caccttatga caatattcaa cctggcatat
     6601 atccaccaat gttggtgact acagggctgc atgatagtca ggtgcagtat tgggagcctg
     6661 caaaatgggt agctaagata agagatattg tccatgatag tcatctgatt ttccttaaca
     6721 ccaatatgga tgccggacat ggtggcgctt cgggcagatt cagacgttat gatgagatag
     6781 cactacagta tgctttcctt attggtttaa atgaaggaat tttaagctaa aatttatgat
     6841 ggttcagaat gttttgttgt attaatcaga tttgattcat acttttggat tatatttgaa
     6901 gaatgtttgt aaaattgaaa ttttggtttt caattttttt cgccattctg ttccttgcca
     6961 atcaactaag tgcagtttgg gttgggctat ctttggagag acacaagtta cagtttcagg
     7021 cgtcgctggg aaaaaatctg ttgagttttt ctgaagaata tcttgcgaaa aatgctacat
     7081 tcagatggat aaaacccgga atcgaattta ccgtcaacca ccaaatgtac gatgtcaagc
     7141 ataccgaaat ggttaaaggt gtcaatgtat ttcactgtaa acatgatgtg cacgaatcta
     7201 aaattctgaa aagattaaag aaaaatctga aagaagactc aggaaaagct cccaaaggga
     7261 atgctaatac agtaaaagta tccatagcaa ccctgttttg ccagagcctg cccatattcc
     7321 aataccttat tttcgaccaa tacaaaactg ttatatt
//
LOCUS       DAOQQY010000126         7350 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4077_length_7350_cov_4.515970, whole genome shotgun sequence.
ACCESSION   DAOQQY010000126 DAOQQY010000000
VERSION     DAOQQY010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4077_length_7350_cov_4.515970"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1599)
                     /locus_tag="PLE23_07205"
     CDS             complement(<1..1599)
                     /locus_tag="PLE23_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83087.1"
                     /translation="MKCTFIPPHRSDVRRTNFDPGGDTYGRGNRMRHSTYKKSWLSTF
                     LTVSAFFLLGLTSVTAQTSTWTGAAGNGFWDDPNNYSPVGLPVATSTVIIPGGATPYP
                     SGYNSMVSMAFRRLDVQNGASATVPNLFTLTLNATASPYGISLGSGSSVTNNHNINIG
                     AAATGIRNQGGTFINNDELYINGATAAGISNSPTGGATNPSVFINNSEITIGNISSGG
                     ANGIFNGGAGALAASFTNANGMISILNVDNAGIRNSALNGPASFINQADIAINANNTL
                     SGFDNEGFINEDNGTTNNTGNLSVDNANRAIVNRMNGTFTNDTGGSIVINPMAPADVN
                     NGIVNILNGTFVNRSAAITMTSLNQIGIWNGAVSSAATFLNTGTGASISINGTAQYAI
                     RNLSDALSVPTTTFKNEDSAVITICDGGVGASTAAIYNGVNASFTNGNSTAGSGALLN
                     VVSAGADGIYNAGTFLNTSYSSVAPFNLSDISVINIGFTNGSCTGNIPGNGITNLNAF
                     TNTEDSRINITAFALSGIENFGTFN"
     gene            complement(2684..6532)
                     /locus_tag="PLE23_07210"
     CDS             complement(2684..6532)
                     /locus_tag="PLE23_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMX83088.1"
                     /translation="MSKTDTLIQSSSKARMAIRHHFSTRTRYYTVILLFLQFFVSDFI
                     YAQQPDCALVCVGKLNVSLDANCEATITADMALKNFWDPQCKPNGPQAFMVFVMYNGD
                     VIPTSPKITADYLGKTLQIKVKHWKTGNTCWTDAFIEDKMPPVLKCPPNVTIACNAPS
                     DPSHTGQATATDCTGYTITHSDITQNFNCNNPAGKITRTWFATDLNGFSSSCTQIITI
                     KKTGINDVVFPKNLDGIQSPALDCSTASTDPSVTGYPTIDGYPINNSSCMVSGTYSDQ
                     KIDICTGTYKILRTWTLVDWCTGQIKTHIQIIKVEDKTGPVITCPNSINGNSYSNQCI
                     GSAVIPAATITDNCSGYSVQVYGSWGGSISGNGGTFNNIPIGNHTVSYIATDDCGNSS
                     SCTSTLKLIDNSPPVVICHDKIIVSIGGNGVSVVNASSFDDGSYDNCCLEKYEVKKMG
                     EPDINFGPSVSFNCDDAGKTINIILKVTDCSGNENTCMTEAEIQDKLPPSIICPPDAT
                     VSCPVDISDLSKYGSATAANNCGNISITETSNIDLSSCGTGKITRTFTATASNGKIAT
                     CTQTIYVNSSTPFTAADITWPKDYDAQGCTSIADLDPDDLPAGYDKTIINNKSCSFAG
                     VTHTDEVFDIAAPACFKILRKWVVIDWCQYDPNKPNSPGRWEHTQIIKVFDTEKPVLD
                     CPDDVTVSSTTTDCSGAQVTLPPLTATDCNPKFTVTNSFNNGGANASGFYPLGTTLVT
                     FTVTDGCKNTSTCVVKVTVTDGKKPTPICLNGLSITIMPSTLSVTIWAKDFISKVLDD
                     CTPQSKIIYKIAKSPSGNQGPPADSSITFTCDDLGKQAVDIWVGDEAGNWDYCTTFID
                     VQDNMNSCPPGSIKNIIMAGKVKTESGKTVENVELSINTSAPKYATTDNKGEFLFPGL
                     KGGNDYTISPTKDINPMNGISTIDLLTIQKHILGIEPLGSPYKLIAADIDKNNNVSTK
                     DLLELRKLILQLIDKFPSNTSWRFIDGNYVFPDPSNPFKTSFPEVLNYPNVKNDMLDL
                     KIVGIKVGDVDGTAKPNEATGGAEVRSEQPELKLSIQDKKLVAGEVVEVPVYLNQTGL
                     EALQFTLSINPEKLEYIDMRASDLTDWTEECVNAQYADKGHIAVAWASVQAFNRIETP
                     IFTLRFRVKSDAILSQNLQITDKITNPLAYTIQKEVLRPGISYEEANMAPTLVSYHNA
                     PNPFRNSTNVYFYLSYNTDVLFKIYDINGKMLLQKRQDGVPGMNSILVDSAELPHSGL
                     YIYEITTPVQRVTDKMILTE"
BASE COUNT         1938 a         1454 c         1599 g         2359 t
ORIGIN      
        1 gttaaacgtt ccgaagtttt caattcccga aagggcaaaa gcagtgatgt taatcctgct
       61 atcttcggta tttgtgaaag cattcaggtt ggttataccg ttgcccggaa tattgcctgt
      121 gcagcttcca tttgtaaatc ctatattaat aacgctaatg tcactcaggt taaatggagc
      181 tacacttgaa tatgaagtgt ttaagaaagt tcctgcattg taaataccat ctgcacctgc
      241 tgaaaccacg ttaagcaatg caccacttcc cgcggtagaa ttgccgtttg tgaaagaagc
      301 attgacacca ttgtaaatag cagcagttga tgcaccgaca ccgccatcgc aaatggtaat
      361 gactgcgcta tcttcatttt taaatgtagt tgtaggtaca gatagtgcat cagataagtt
      421 tctgatggca tattgtgctg taccatttat cgaaatagaa gctcctgtac ctgtattcaa
      481 aaatgtcgct gccgaagaga cagctccgtt ccaaatacct atctggttaa gggatgtcat
      541 cgtaatagca gctgatcggt tgacgaaggt accattcaga atgtttacaa taccattgtt
      601 tacatctgcc ggagccatag ggtttataac gatcgaacca cctgtatcat ttgtaaatgt
      661 accgttcatt ctgttgacaa tagcacggtt ggcattgtcc acagagagat ttcctgtatt
      721 attagtcgtt ccgttgtctt cattgataaa tccttcgtta tcaaatccgc ttagtgtgtt
      781 gttagcatta atggcaatgt cagcctgatt gatgaagctg gcaggaccgt tcaaagctga
      841 gtttcggatt cctgcattgt caacattcag gatgctgatc atgccgtttg catttgtaaa
      901 gctggcggcc aatgcacctg cgccaccatt gaatataccg ttggcaccac cactactgat
      961 gttacctatg gttatttcac tgttgttgat gaaaaccgaa gggttcgtgg cacctcctgt
     1021 tggagagttg ctgatacctg ctgcggttgc tccgtttatg tagagctcat cattgttaat
     1081 aaacgtgcca ccctggtttc tgatacctgt tgctgctgca ccaatattga tattgtggtt
     1141 gttggtcact gaagaccccg aaccaaggct aataccatac ggtgatgctg ttgcattaag
     1201 tgtaagggtg aataaatttg gaaccgttgc tgaggctccg ttctgtacat ccagccgcct
     1261 gaatgccata cttaccatgg aattataacc actcgggtaa ggagtggcac ctcccggtat
     1321 aatgaccgtc gaagtggcta ccggcagtcc taccggtgag tagttgtttg ggtcatccca
     1381 gaagccgttg ccggctgcac ctgtccaggt tgacgtctgg gcagtgacgg atgttaaccc
     1441 aagtaaaaag aatgcactta cagtcaaaaa cgtgcttaac cagctttttt tgtatgtaga
     1501 gtgcctcata cgattacctc gcccgtaagt gtcacctccc gggtcaaagt tggtcctcct
     1561 aacatctgaa cgatgggggg gaatgaaagt acatttcatc gttaaaaggt ttagttaaaa
     1621 aatattgtta atgtcatttc taagctgttg gtcctaaaat cctaaaagca gagaatcccc
     1681 gttccgtgac atgtatgatg ctgtacggat aatgcgaatt ctccaagctt ttaggctaaa
     1741 caaggacgat gaaccttccg caaagacata gtggtgacac catgcttacg gaattacgta
     1801 gcacaaattc ttttgcaggt agtatgcagg atgtggtatg agtcagtttt gtccggcttc
     1861 gaaaagcaaa tggggggtac ggatacactg atcttgtaca atccggcaat cactggatta
     1921 cattgtaatt tttttattgg aattcaggtc aaaacaacca gagttaactc tggattaatc
     1981 ggtttttggg aatacggttt tgggagagat tttgggatgt tgggctacat ttagtttctt
     2041 aaatggccgg tgttttagtt gttacctgat tcgccgatta attttcatat tattacattt
     2101 tacatatata tacatttata atatgtaaaa ggtttcaaaa cattcaatta aaatttattt
     2161 ttttttgtaa ttaattaaat atgattgatt ccttgtattt gttacttgaa acggagctgc
     2221 tattattagg ttccgctact gcattcaaat attataaaat atataatatg aattatgcaa
     2281 cggtatcata tcagcaagga atggtagagg ttgtggtttg aataaaatgg catgagcagg
     2341 aattgaataa tccttggcaa tgtctgctca ccttttagta taaaatattg cccggtaggg
     2401 tgaaaaaacc attctcacca taataacata gatagatgtc acgaaaaatt gaagtagaag
     2461 ggaaggtact tttatgtgct ttgcgaatgt agttgaacct gatatctatt atattaagta
     2521 tagtatctgt gttttcatac atcagcaatg catactaata tgtcaagaag agatatgtaa
     2581 agtaaatagt ggaaagcgat gcgtagcatg gacgatttgt caaaataaaa aagccggaat
     2641 atttgcatat tccggccaaa ccccaaaacc atataaccgt taattattcg gttaaaatca
     2701 ttttatctgt caccctttgt acaggagtgg ttatttcata aatatacagt cctgaatgcg
     2761 gcagttcggc actatccacc agaatgctgt tcatgcccgg aacaccgtct tgtcttttct
     2821 gtaatagcat tttaccattt atatcgtata ttttaaataa tacatcggta ttatatgata
     2881 aatagaaata cacattcgtt gaattcctga atgggttagg cgcattgtgg taacttacaa
     2941 gggttggtgc catgtttgct tcttcatagc taatacccgg gcgcagtact tctttttgta
     3001 ttgtgtaagc aagcggattg gtaatcttgt cggtaatttg taaattctga ctcaggatag
     3061 catctgattt taccctgaac cgtagcgtaa atatcggtgt ttctattcta ttgaaagctt
     3121 gtacagatgc ccaggcaact gctatatgtc ctttatcggc atattgtgca ttgacacatt
     3181 cttctgtcca gtcggttaag tctgatgcgc gcatatctat atattccagc ttctctggat
     3241 tgatgcttag cgtaaactgc agtgcttcga gaccggtctg gttgagatac accggtactt
     3301 ccactacttc acccgctacg agctttttat cctgaatact cagcttcagc tctggttgtt
     3361 cgctgcgtac ttcggcacca ccagttgctt cgtttggttt ggcagttccg tccacatcac
     3421 ccaccttaat acctacaatc ttgaggtcga gcatatcatt tttcacattc ggataattga
     3481 gcacttcagg gaacgatgtc ttgaatgggt tcgatggatc tgggaataca taattgccgt
     3541 ctataaaccg ccagcttgta ttggaaggga atttgtcaat aagctgaagg ataagtttcc
     3601 tcaattcgag caggtctttg gtagagacat tattattttt gtcaatgtcg gctgctatca
     3661 atttataagg tgaacccaag ggctcgatac cgagtatatg tttttgaatg gtaagcaggt
     3721 caatggtaga gataccgttc attggattga tgtctttggt tggtgagatg gtataatcat
     3781 ttccgccctt caatcccggg aacaagaatt cacccttgtt gtctgttgtg gcatatttcg
     3841 gtgcacttgt attgatgctc agctcaacgt tttctacagt tttgccggat tctgtcttta
     3901 ctttaccggc catgatgata tttttgatag accccggtgg acagctgttc atattatcct
     3961 gcacatcgat gaaagtagta cagtaatccc aattgccagc ttcatcacca acccagatgt
     4021 ccacagcctg tttgcccaga tcgtcacaag tgaatgtgat gctcgaatcg gccggagggc
     4081 cctggttgcc tgaaggtgat ttggctattt tgtaaataat ctttgattgc ggagtacagt
     4141 catccaatac ttttgatatg aaatctttag cccagatggt tacggacagt gttgaaggca
     4201 tgatggttat cgacagtcca ttcagacata tcggtgttgg ttttttgccg tctgtcactg
     4261 ttaccttcac tacacaggta gaagtatttt tacagccatc tgtaactgtg aatgtaacga
     4321 gagtagtacc aagaggatag aatccgcttg catttgcgcc gccgttgttg aagctgttgg
     4381 tgacagtaaa tttgggatta caatctgtag ctgtcaatgg agggagggtt acttgtgctc
     4441 cgctgcaatc ggttgtggta ctactcaccg ttacgtcgtc aggacaatca agtaccggct
     4501 tttctgtgtc gaataccttg atgatctgag tgtgctccca gcggcccgga gagtttggct
     4561 tgttggggtc gtactgacac cagtctatta ctacccattt tcttaatatc ttgaaacatg
     4621 caggagctgc aatgtcaaat acttcatctg tgtgtgtgac acctgcaaaa ctgcatgatt
     4681 tgttgtttat gatggtcttg tcatatccgg ccggcagatc atcagggtca aggtcagcaa
     4741 tggatgtaca accctgtgca tcataatctt ttggccaggt aatgtcggct gcggtaaatg
     4801 gcgtactgct gttgacgtag atggtctgtg tgcatgttgc tatctttcca ttgcttgcag
     4861 tagcagtaaa ggtacgtgtt atctttcctg ttccgcagct tgagagatca atattgctgg
     4921 tttctgtaat gctgatattg ccacagttat tggcagctgt cgccgaacca tatttgctca
     4981 ggtcgcttat atctactggg cagctcactg tagcatccgg tgggcagatg atggatggtg
     5041 gcagcttgtc ctgaatttcg gcttctgtca tacaggtatt ttcattgccc gaacagtcgg
     5101 ttaccttcag tatgatgttg attgttttac ctgcatcatc acaattgaag ctcacactcg
     5161 ggccaaagtt gatgtctggc tctcccattt tttttacttc gtatttttcc aggcagcaat
     5221 tgtcataact accgtcatca aacgaagatg catttactac agaaacaccg tttccgccga
     5281 tacttactat tattttatca tgacaaataa ccacaggtgg gctattgtca atgagcttga
     5341 gagtagatgt gcagcttgaa ctgttgccac aatcatctgt tgcaatgtag ctcactgtat
     5401 ggtttcctat cggaatgttg ttaaacgtac caccattgcc actgatactg ccaccccaac
     5461 tgccatacac ttgcactgag taacccgaac agttgtcggt tatagtggct gccggtatca
     5521 ctgccgagcc aatacactgg ttgctgtaac tattaccatt aatactgttc ggacaagtaa
     5581 tcacaggtcc ggttttgtct tctaccttga tgatctgtat atgtgtctta atctggcctg
     5641 tgcaccagtc aacaagggtc catgtgcgga gtatcttgta ggtgccggta caaatatcta
     5701 ttttctgatc gctgtatgtg cccgatacca tacagctcga gttgttgatc ggatatccgt
     5761 caatggtagg gtagccggtc actgatggat cagtacttgc agtgctacaa tcaagtgccg
     5821 gtgactgtat gccatccagg tttttcggaa aaacaacatc gttgatgccc gttttcttga
     5881 tagtaatgat ctgagtacat gagctggaga agccattcaa gtcggtagca aaccaagtac
     5941 gggtaatttt gcctgccgga ttgttacagt tgaagttttg tgtgatgtca gaatgtgtga
     6001 tagtatagcc tgtacagtct gtagctgtag cctggccggt atgcgaaggg tcacttggtg
     6061 cgttgcatgc tatcgttaca tttggcggac actttagtac cggaggcatc ttgtcttcaa
     6121 tgaaggcatc tgtccaacag gtattgccag ttttccagtg tttaaccttt atctgaaggg
     6181 tttttccaag atagtccgct gttattttag ggcttgtagg tatgacgtca ccattgtaca
     6241 tcacgaatac cataaaagcc tgtggaccat tgggtttgca ctgggggtcc caaaaattct
     6301 tcaatgccat atcagctgta atagtagctt cgcagtttgc atcaagtgaa acattcaact
     6361 taccgacgca gactaaagcg caatccggtt gttgggcgta aatgaaatca gatacaaaaa
     6421 actgtaaaaa caacagaata accgtataat accttgtacg tgtagaaaaa tgatgacgta
     6481 tagccatcct tgcctttgag cttgactgaa taagtgtatc ggtcttcgac atgggacgta
     6541 tgtttaaaaa gttaaaaaat actatctcat gctattgggt gtccacaccg cgtattactt
     6601 tgtaatgtca atacaccgtt gcatacctat agctttggga atttttgttc cgtttcagga
     6661 tttttaactt atgtttctca ggagaatgga aaacagcctt caggagaata ttttaccgga
     6721 ctgtatctgg cggctgatga ctcaaccgga atcggatgtg cttagtctga tatttttcat
     6781 gggcggtttt ttcaaaaatt ggttttcaaa atatgttctt tgttgattga tcgcttccgc
     6841 aatacaatgc aaatgtagga gcattacgct ttcctgtcga taccctgcaa ttggtatttt
     6901 tatattgaaa ttaattatat taatttatta tcacaaccaa aagtttgtaa aatatttata
     6961 atcaattatc aaaacattca ataataaata aattataata atattaatta aacataattc
     7021 attgtctttt gaaaaattaa ttgagtacct gtactttgtt atgctgctgt ttctgttttt
     7081 ttagagaaaa tcgtgcattc ctccatgtcg tctttttaat atgagcgaaa caaaaaaggc
     7141 cggctcacaa gtggagtccg acctttatag aaatgtgtca ggtaggtatt aatttcttta
     7201 tttgatgaga atcattcttg ctactgcttt ctgtttttct gtctcaagag agtacatata
     7261 aagacccgga ccgttgagtt cttttgcatc aaggtaaatc tcatggtatc cttccggcag
     7321 ttgtagctgt ttttcgatga atgtcttacc
//
LOCUS       DAOQQY010000127         7341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4084_length_7341_cov_5.360829, whole genome shotgun sequence.
ACCESSION   DAOQQY010000127 DAOQQY010000000
VERSION     DAOQQY010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4084_length_7341_cov_5.360829"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PLE23_07215"
     CDS             complement(<1..229)
                     /locus_tag="PLE23_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMX83089.1"
                     /translation="MALNQEFVEMSKEPVRILIVDDEEDITEFLSYNLKKENYHVLCI
                     NDSTKAIATAESFRPDLIVLDIMMPVMNGIEV"
     gene            complement(244..573)
                     /locus_tag="PLE23_07220"
     CDS             complement(244..573)
                     /locus_tag="PLE23_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HMX83090.1"
                     /translation="MINRLFIYILICGLSVACQKTEKPQVDKDTMVKILADMHVSDAV
                     LANVNPGIKDSVSKVYLDEILKIHNVKKTDFDKAIKYYESKPEILETMYEDVLKRLEN
                     GTTDNDK"
     gene            complement(563..1057)
                     /locus_tag="PLE23_07225"
     CDS             complement(563..1057)
                     /locus_tag="PLE23_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMX83091.1"
                     /translation="MKELIFIRHAKSDWNNPLLSDINRPLGPRGLKDAPIMAARLRSL
                     VPAVDHIVSSNAVRALETSSFFVKEYGMNIHGIESVPELYHAYPDEILHEVRQLNQLH
                     RCVLFFGHNPGYTHLANMFSDYMIDNVPTCAFFKLEIHADSWKEISPDNTRLTLFSKP
                     RDDK"
     gene            1152..2837
                     /locus_tag="PLE23_07230"
     CDS             1152..2837
                     /locus_tag="PLE23_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83092.1"
                     /translation="MNFQLKDLHLHFDKLTMAEAEMLITSGDLQKISEIQKNVWQLKF
                     DYENVNSRCELNTSGKVKKYSCSCVVFSLYKKCKHVAALCMLIQKIKLNASTKDDNPL
                     NRTVRSISLGKILKHTSHIELTNFLLDYTKTSPELSALLKARFLFNFPEEITLENDPI
                     LTAQINRLLQSKRPAENTLFDIYKSLDAYFVQAIRKIDQHETLAAFNILSIISKNLNK
                     LIVQYQADNEKLLGMSIKLYNKLADLNRLFVAPEAKNQLSELLLEGRELTTTITNTEW
                     HEKYIEVLLLNLSNNSDFQNIKDELFNKYFKQIRLIENRSAWIMFDIQSDVLCNVKPE
                     PTNIATNIGEDIDLLRSLLTRLIPKLHYELASKLITQCCKQYPTYFSTYTHELNSIQL
                     EMAVRTHNWKKAEKLLLSDFPHNPDGRVLLYIKEAFPTHFEEISNKLSQIKLPDTEQQ
                     LLLEMVLSEVSGDYLAPMNTIIASNDIRLLVKYDHLFWKKNPEETVELYKTLSKLYLE
                     THFGEPAQTFIHDMFQHLENNKRKEEVRTIRKFLNPLFSKRMAINTKIKYPAI"
     gene            2834..3307
                     /locus_tag="PLE23_07235"
     CDS             2834..3307
                     /locus_tag="PLE23_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HMX83093.1"
                     /translation="MKLELWCIGKTAETYLDEGIDIYRKRINKYVPFEMRIIPDVKVN
                     GKPDAAHLMLQEATLVQKLLSKDDYLITLDERGKTFSSVMFSEQLNRLVQLPYKRIIF
                     LVGGAWGIHDTLKQKAGMMLSLSQMTFSHQMIRLFFTEQLYRAFSILNNEPYHNE"
     gene            3311..3922
                     /locus_tag="PLE23_07240"
     CDS             3311..3922
                     /locus_tag="PLE23_07240"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMX83094.1"
                     /translation="MITYILMTTIGIVSYMAFSDQSMKHKLAFYPYGAFHHKEYSRFI
                     TSIFVHADWTHLLLNLFVLYGFGLAMEREFKDLSIQLYGSPQVGYLHFLAIFLLSGIV
                     GDLPDYFMKRNMTHYLSLGASGAISGLLFAFIMLHPTARMVVFIGIPVPMVAWLYGIM
                     YVAFSIYASKKNSDNVNHLAHLGGALTGILYVLFIFPGIKLPV"
     gene            3919..4425
                     /locus_tag="PLE23_07245"
     CDS             3919..4425
                     /locus_tag="PLE23_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83095.1"
                     /translation="MRKLFIALTLTVCFIACKDNKPKDCPSGKPLPIFTKEMSVVKNH
                     SFNAQGQKSQEEVGLANGTRLEIFQQGCDTLSQSFRFSFSHLITRDSFVTTIDSAISQ
                     FRYLSAANEKLKPFVIWSDALTQLRTHIRQGEYLDLDRGIKLRIDQLDQGSGTTIVLD
                     VMQISSTH"
     gene            4473..5870
                     /gene="lpdA"
                     /locus_tag="PLE23_07250"
     CDS             4473..5870
                     /gene="lpdA"
                     /locus_tag="PLE23_07250"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMX83096.1"
                     /translation="MNFDLIVIGSGPGGYVAAIRASQLGMKVGVIEREHVGGICLNWG
                     CIPTKALLKSASVFEYINHAADYGITTGTATADFPKVIQRSRDVAGGMSKGIHFLFKK
                     NKITLIDGHATLNKNKSVDVKDAAGKVTNYTANNIIIATGGRAKELPNLKIDGNKIID
                     YKKAMSLESQPKRLVVVGAGAIGVEFAYFYNSIGTEVTVVEFMEQGLLPREDADISKE
                     LSKIFTKKGIKVLANTGVEKVDTSGNECKVFVKSRKDGKEETITCDVVLSAAGVTPNT
                     ENIGLEALGIKTDRGMILVDQYYKTNVDGIYAIGDVIPTPALAHVASAEGILCVEKIA
                     GIHVEPIDYNNIPSCTYCSPEVSSVGYTEKSAKEAGYDIKVGKFPFSASGKASAAGAK
                     EGFVKVIFDAKYGEFLGAHLIGMNVTEMIAEVVVARNLETTGHQIIKSIHPHPTMNEA
                     IMEAAAAAYGEVIHM"
     gene            complement(5944..7053)
                     /locus_tag="PLE23_07255"
     CDS             complement(5944..7053)
                     /locus_tag="PLE23_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HMX83097.1"
                     /translation="MKKILKISYVLVLLVCLTTKANAYSSPGHWIIAKIAFDRLDPAV
                     RSNVLQALDGMSFEQASTWMDDVRQDKNYTYLRSWHYINIPAGESYKPTEGDNLMNAL
                     HRAFDDFRSGVAVNEEQLKLNTLLVFHLIGDLHQPLHVGYADDRGGNDYQVNINGYGT
                     NLHKVWDEYLISKQKVTYESCTALLNGLPEQDVKTIAQTDMVAWWNESRALLGDIYNI
                     GGEHKMSDDYLVRMKPVVEMQLVKAGVRLAGVLNEFFKEPIAVTSTPPAEKRVIEDAD
                     AINHVGEKVTVCGKVYTTRFLDYSAKKPTFLNLGAAYPNQTFTIVIYGPSREKFSYQP
                     EERLKNQNVCVTGRVQLYKNKPQIILNEPEQLKIQ"
     gene            7106..>7341
                     /locus_tag="PLE23_07260"
     CDS             7106..>7341
                     /locus_tag="PLE23_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83098.1"
                     /translation="MQTISICIAIAWAYPCRVGLSALLRAGFYPLRISFSKKFEYPEQ
                     NYQSHLIENAPSPVSLNTGLQKPRSFMIRGFGFL"
BASE COUNT         2214 a         1742 c         1367 g         2018 t
ORIGIN      
        1 agacttcaat accgttcatc accggcatca taatatcaag tacgataaga tcgggcctga
       61 aagattctgc agtggcaatg gcttttgtac tgtcgttgat gcatagtaca tgataatttt
      121 ccttctttaa gttatagctt aaaaattcgg tgatatcttc ctcatcatca actattaata
      181 tcctgacagg ttcctttgac atttctacaa attcttggtt aagggccaat tgaggaatcc
      241 tttttacttg tcattatccg ttgtaccatt ttcgagccgt ttaagcacat cttcgtacat
      301 ggtttcaagt atttcaggct tgctttcgta gtatttgatg gctttgtcaa aatcagtctt
      361 ttttacattg tgaatcttca atatttcatc aaggtaaact ttcgaaacgc tatcttttat
      421 accaggattc acattggcca aaacagcatc cgatacgtgc atatctgcca atatttttac
      481 cattgtatct ttgtccacct gaggtttttc agtcttttgg caggcaaccg acagaccaca
      541 tatcagtata taaataaata gtctatttat catcgcgagg ttttgagaat aatgtgagcc
      601 ttgtattgtc aggtgaaatc tctttccagc tatcggcatg tatttcgagc ttaaaaaaag
      661 cacaggtagg cacattgtca atcatatagt cagaaaacat atttgccaga tgagtataac
      721 ccggattatg cccaaagaac aatacacaac ggtgcagttg attcagttgt cttacttcgt
      781 gaaggatctc atccgggtaa gcatgatata attctggtac agattctatt ccatgaatat
      841 tcataccata ttctttgaca aaaaaagaag acgtctccaa agcccttact gcattgcttg
      901 acacgatgtg gtcaacagcc ggcaccaatg acctgagtct cgcagccatt ataggtgcat
      961 ctttaagtcc acgcggtccc aaaggacgat tgatatccga caaaagagga ttattccagt
     1021 cagattttgc gtgtcttatg aaaataagtt ctttcatata gcaaatgtag tataatatta
     1081 attttatgtg tagtagcttt accttttaaa tcaatttttt cgtacaattc ttacagcaag
     1141 cattttagtg tatgaatttt caactcaaag acctgcacct tcatttcgat aagctgacca
     1201 tggcagaagc cgagatgctg attacttcgg gtgatctaca gaaaatatcc gaaattcaaa
     1261 aaaatgtctg gcagttgaag tttgattacg agaacgtaaa tagccgatgc gagctgaata
     1321 catccggtaa agtcaagaaa tattcatgca gttgtgtcgt gttttcactt tacaaaaaat
     1381 gcaaacacgt tgctgcactg tgtatgctca ttcagaagat caaactaaat gccagcacaa
     1441 aggatgacaa tccattaaac cggactgtcc gttccatttc cttaggaaag attcttaaac
     1501 atacttcaca tattgagctg actaattttt tactcgacta taccaagact tctccggagc
     1561 ttagcgcact gctcaaagca aggtttctat tcaacttccc tgaagaaatt accctggaga
     1621 atgacccgat cctcactgcc cagatcaatc ggttgttaca gtcgaaaagg ccggcagaaa
     1681 atacactttt cgatatttac aaatcgttag acgcttattt tgtccaggca atccggaaga
     1741 ttgaccaaca tgagacactt gctgcattca atattctttc tatcatcagc aaaaatttga
     1801 acaaactcat cgttcaatac caggcagata atgaaaaatt gctcggtatg tccataaagc
     1861 tatataacaa actcgctgac ttaaaccgat tgtttgtggc tcctgaagca aagaaccaac
     1921 tatcagaatt actactcgaa ggcagagaac tcactaccac aataacaaat actgagtggc
     1981 atgagaaata tatcgaagtt ctgttattaa atttatccaa caatagtgat tttcaaaata
     2041 ttaaggatga attattcaac aaatacttta aacaaatacg ccttattgag aaccgatcag
     2101 catggataat gtttgacatt cagtctgatg tactctgtaa tgttaaaccc gagccgacaa
     2161 acatcgccac caacataggc gaagacatag atttgcttcg ctcgcttctc actcgcctca
     2221 tccccaagct acattatgag ttggcaagca aattaatcac tcaatgctgc aaacagtatc
     2281 cgacatattt ttcgacctat acacatgaac tcaactccat tcagctcgag atggcagtac
     2341 gtacgcacaa ctggaagaaa gctgaaaaat tattgctatc cgactttcct cataatccgg
     2401 atggtcgtgt cttattgtat ataaaggaag catttccaac gcattttgaa gaaatatcca
     2461 acaaactcag tcaaattaaa ttacctgata ccgaacaaca actcttactt gaaatggtac
     2521 tttcggaagt tagcggcgat taccttgcac cgatgaacac catcatagca agtaacgata
     2581 taagactact tgtaaaatac gaccacttgt tctggaaaaa aaatccggaa gaaacggttg
     2641 aactctacaa aacactctct aaactttacc ttgaaaccca ctttggcgaa cccgcccaga
     2701 ctttcatcca tgatatgttt cagcacttgg agaataataa acgcaaagaa gaagtaagaa
     2761 ccatccgcaa attcctcaat ccgttgtttt caaaaagaat ggccatcaac actaaaatca
     2821 aatatcctgc gatatgaaac tcgagctttg gtgtataggc aaaacagcag aaacctacct
     2881 agatgaagga atcgacattt accgtaaaag gataaataaa tatgtaccat tcgaaatgcg
     2941 gataatacca gatgtaaagg taaatggcaa accggatgca gcacacctca tgttgcaaga
     3001 ggcaaccctg gtacagaaac tactttctaa agacgattac ctgataacgc tggatgaacg
     3061 aggcaaaacc ttcagttcgg tcatgttttc agaacaactc aaccgacttg tccaactacc
     3121 atacaaaagg attatcttcc tggtaggagg cgcttggggc atacacgata cactcaaaca
     3181 aaaagcagga atgatgttat ctttgtcaca gatgacattt tcacaccaga tgatcagatt
     3241 attttttaca gaacagttat acagagcatt ctccatcctc aacaatgaac cataccacaa
     3301 cgaataaaat atgataacat acatattaat gaccaccatc ggcattgtta gttatatggc
     3361 ttttagcgac cagagcatga agcacaaact ggccttttac ccttacggcg catttcacca
     3421 taaagaatat agccgattca taacctctat tttcgttcat gccgactgga cccacctatt
     3481 gctcaatctc tttgtgttgt acggcttcgg acttgccatg gagcgagagt tcaaagatct
     3541 ctctattcag ctttatggtt caccacaggt tggctacctt catttccttg ctatcttcct
     3601 attgagtggt attgttggtg acctgcccga ttatttcatg aaaaggaata tgacgcacta
     3661 tttgtcctta ggagcatcag gcgctatatc gggcttactt tttgccttta tcatgctcca
     3721 ccctacagca cgcatggttg tattcatcgg cataccggtg cctatggtag cctggcttta
     3781 tggtattatg tatgtagcgt tttctatcta tgccagtaaa aaaaacagcg acaatgtcaa
     3841 tcacctggca catttgggtg gtgcccttac aggtattttg tatgttttgt ttatttttcc
     3901 gggaataaaa ttaccagtat gagaaaattg ttcattgcct tgacactgac tgtgtgcttt
     3961 atcgcctgta aagacaataa accaaaagat tgcccgagtg gcaaacctct acctatcttc
     4021 accaaggaaa tgtccgtagt caaaaaccat tccttcaatg ctcaaggaca aaagagccaa
     4081 gaagaagttg gcctcgccaa cggtacacga ctcgaaatat tccaacaagg ttgcgacact
     4141 ttatcacagt cttttcgctt tagcttttcc cacttgataa cccgtgactc atttgtcacc
     4201 actatcgatt ctgccataag ccagttcaga tatctgagtg ctgccaatga aaaactcaaa
     4261 ccattcgtca tctggtctga tgctctcacg cagctacgca cacacatccg acaaggcgaa
     4321 tacctcgacc tagaccgtgg tatcaaactg cggattgacc aacttgacca aggcagtggc
     4381 accacaattg tactggatgt catgcaaatt tcatcaacac attagatttc cgaaaaaatc
     4441 tgtctatctt tacaccaaca aacaaaaatg ttatgaattt tgatcttatt gtaataggca
     4501 gcggacctgg aggttatgtg gctgccatcc gagcgtcaca gctgggcatg aaagtaggcg
     4561 tcatcgaacg cgaacacgta ggcggtatct gcctcaactg gggttgtatc cctaccaaag
     4621 cactcctgaa aagtgccagc gtatttgaat acattaacca cgcagcagac tacggtatca
     4681 ccactggtac tgccaccgcc gattttccta aagtcataca gcgtagccgc gacgtagcag
     4741 gtggcatgag caaaggaatc catttcttgt ttaagaaaaa taaaataaca ctcattgacg
     4801 gccatgccac actcaacaaa aacaaaagtg ttgatgtcaa agacgcagca ggcaaagtaa
     4861 ccaactatac tgccaataac atcatcattg ccacaggtgg ccgtgctaaa gaactaccaa
     4921 atctgaaaat agacggcaac aaaattatcg actataaaaa ggccatgtca ctcgaatcac
     4981 aacctaagcg gctggtagta gtaggtgcag gtgccatcgg tgtcgagttc gcctatttct
     5041 ataactccat cggtacagaa gtcaccgtcg tcgaattcat ggaacaaggc ctgctcccgc
     5101 gcgaagatgc agacatctca aaagagcttt ccaagatatt taccaagaaa ggcattaaag
     5161 tacttgccaa caccggtgta gaaaaagtag atacctcggg caatgagtgt aaagtgttcg
     5221 tcaaaagccg caaagacgga aaagaagaaa ccatcacatg cgatgtggta ctttcggcag
     5281 caggcgttac acccaacaca gagaacatcg gcctcgaagc actcggcatc aagaccgacc
     5341 gtggcatgat actcgttgac cagtattaca aaacaaacgt tgacggcatc tatgccatcg
     5401 gtgacgttat accaacacca gccctcgctc acgtagcaag tgccgaaggt atattgtgtg
     5461 tagaaaagat agcaggcata cacgtagaac ccattgacta caacaacatt ccgtcctgca
     5521 cctactgctc acctgaggtc tcttcagtcg gctatacaga aaagtctgcc aaagaagcgg
     5581 gctacgacat caaggttggc aagtttccgt tctctgcatc gggcaaggca agtgcagccg
     5641 gtgccaagga aggctttgtc aaggtcatat tcgatgccaa atatggcgaa ttcttaggcg
     5701 cacacctcat tggtatgaat gtaaccgaaa tgattgctga agtcgttgtc gccagaaacc
     5761 tcgagactac cggccaccaa atcatcaagt cgatacaccc acaccctacc atgaacgaag
     5821 ccatcatgga agcagcagca gcagcttacg gtgaggtcat ccacatgtaa tcagttccat
     5881 acaagtacat aacacaaagc ccgttggtct gcatcagtca acgggctttt tatttcattt
     5941 ccgttactgt atcttcagtt gctcaggttc gttcagtatt atctggggct tgtttttata
     6001 caactgtacc cttccggtga cacacacatt ctggtttttg agtctttcct ccggctgata
     6061 actaaatttc tcacggctcg gcccatagat cacaatcgta aatgtctggt tagggtatgc
     6121 cgcacccaga ttcaaaaacg taggcttctt ggccgaataa tcaagaaaac gcgttgtata
     6181 gacctttcca cacacagtta ccttctcgcc tacatggttg atggcatctg cgtcttctat
     6241 cacccttttc tctgcaggtg gcgtacttgt taccgctatt ggctctttaa aaaactcgtt
     6301 cagcacaccg gcaagcctta cacctgcctt taccagttgc atctctacca ccggcttcat
     6361 ccttaccaga taatcatccg acattttgtg ttcaccgccg atgttgtata tatcacccaa
     6421 caatgccctc gactcattcc accacgctac catgtcggtc tgggctatcg ttttcacatc
     6481 ctgttcaggc aaaccattca ataatgccgt acaactctcg taagtcactt tttgcttaga
     6541 tatcaggtat tcgtcccata ccttatgcag attcgtacca taaccattga tattcacttg
     6601 ataatcattt ccgccacggt catccgcata gcctacatgc agtggttggt gcagatcacc
     6661 tatcaggtga aaaacgagta aagtattcag cttcagttgc tcttcattga ccgccacacc
     6721 cgaccgaaaa tcatcaaaag ccctatgcag agcgttcatc agattatcac cctccgtagg
     6781 tttgtatgac tcccctgccg gtatatttat atagtgccag cttcgcaaat aagtataatt
     6841 tttatcctgc ctcacatcat ccatccacgt acttgcctgc tcaaagctca tcccatcgag
     6901 agcctgcaat acattcgacc tcaccgcagg gtcaagcctg tcaaatgcta tcttcgctat
     6961 tatccaatgc cccggtgagc tgtacgcatt cgcctttgtc gtcaggcata ccaatagtac
     7021 cagcacataa ctaatcttca atatcttctt catagccgct tataatagca caaagataat
     7081 gaatgtagca ctaaaatgcc agtttatgca aacgattagc atttgcatag caatagcttg
     7141 ggcgtacccc tgtcgggtcg ggctatccgc cttacttcgt gccggcttct atcccttacg
     7201 catttcattc agtaagaaat ttgaatatcc agaacaaaat tatcaaagcc acctaataga
     7261 aaacgcccca agcccggtgt cgctgaacac cggattgcaa aagccccgtt cgtttatgat
     7321 ccggggcttt ggttttttaa g
//
LOCUS       DAOQQY010000128         7325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4097_length_7325_cov_5.033563, whole genome shotgun sequence.
ACCESSION   DAOQQY010000128 DAOQQY010000000
VERSION     DAOQQY010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4097_length_7325_cov_5.033563"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="PLE23_07265"
     CDS             <1..420
                     /locus_tag="PLE23_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692173.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HMX83099.1"
                     /translation="VLKFEEREGRRPRILVAKLGQDGHDRGAKVIATAFADLGFDVDI
                     GPLFQTPQEAARQAVENDVHILGISSLAAGHKTLVPETIEALKNLGREDILVVVGGVI
                     PQQDYDYLFEKGVAGIFGPGTVISKAASGILKLFLEN"
     gene            complement(438..1106)
                     /locus_tag="PLE23_07270"
     CDS             complement(438..1106)
                     /locus_tag="PLE23_07270"
                     /inference="COORDINATES: protein motif:HMM:NF021788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMX83100.1"
                     /translation="MSGKYQFETTVLTLTIAGVLIKLRTVLHPETLFDQMLEKGNNHD
                     DVVNERIPYWAELWPASIAMAEFLLNNRHLSEGKRLLEIGCGLGLAGIGANLSNAQVT
                     ISDYLQEAVDLAKINWRLNFDTPPNVRLLDWHFPPDGETFEIIIGADVLYDKMNHSAL
                     SIFFDKMLVPGGIIILTDPKRSATGRFFELPKMQEYQNQRHTITVTNKGATSEIDIFV
                     LQKI"
     gene            1240..3165
                     /gene="thrS"
                     /locus_tag="PLE23_07275"
     CDS             1240..3165
                     /gene="thrS"
                     /locus_tag="PLE23_07275"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMX83101.1"
                     /translation="MINITFPDGNVRQYETGTTPLSIAKSISEGLAKRILVASINGQI
                     WDMSRPVYEDSNIKLLSWDDTDGKSTFWHSSAHLMAEAVEAMFPGVKFWVGPPIDKGF
                     YYDMDLGDAQITEDDLVKLEKKMTELAKQNNLYTRQEVSKADAVAYFTEKGDEYKLDL
                     LENLEDGEITYYTQGNFTDLCRGPHIPHTGLIKSIKLTNISGAYWKGDEKNKVLTRVY
                     GVTFPNQQEMDEYLHMLEEAKKRDHRKLGKELGIFTFDDDVGPGLPLWMPNGTIIIEE
                     LEKLAKETEEAAGYKRVVTPHIAKESMYLTSGHLPYYQDSMYPPMEMDGTKYYLKAMN
                     CPHHHKIFDAEPRSYRDLPLRLAEYGTCYRYEQSGELFGLMRVRCLHMNDAHIYCTKE
                     QFFEEFRAVNDMYLKYFKIFGIEKYVMRLSLHDPAKLGQKYINEPELWLETEEMVRQV
                     LKDTGTPFVEVADEGAFYGPKIDVQIWSAIGREFTLATNQVDFAQGRRFNLSYTTSDN
                     SHDTPLIIHRAPLGTHERFIGFLLEHYAGRFPVWLSPVQVKVLPISDKVADFSKNVFQ
                     SLKSAGIRVEIDNRVESIGKKIREAELMRVPYMLIVGEKEAAEGKVSVRKQAEGDLGQ
                     QTIAEFVANMREWSNRQ"
     gene            3262..3603
                     /locus_tag="PLE23_07280"
     CDS             3262..3603
                     /locus_tag="PLE23_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83102.1"
                     /translation="MKRILLSIFLVLSMVFCAFSQSSSDGFGSSASYNENIKVSVYPN
                     PASDYINIQDKTNTITSINVFDLAGRKIKTFKTEGIQMLSIVDLPKGIYLMQFLDKKE
                     KLVTTQRLSKK"
     gene            complement(3663..5108)
                     /locus_tag="PLE23_07285"
     CDS             complement(3663..5108)
                     /locus_tag="PLE23_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMX83103.1"
                     /translation="MIVNEPGYEYLNAETHLSAVFNKMKQNVDSVKISNASQRILKLK
                     LLKESILSHQNEMREAMNISFKKPALETDITELLPVISHINLVVKNLSHWMKPERRPT
                     PKELLGAKSYVIHEPKGIVLILSPWNYAFNLLFDPLVSAIAAGNCAILKPSEATPAAV
                     KVIKKIVEAVFPPNEVYVAEGDARTASYLTSLPFDHIFFTGSPAIGRLVMKAAAENLA
                     SVTLELGGKTPAIVHQDADISKTANSLVFAKFLNTGQTCIAPDYLLVHKDVVSKLSSA
                     LIERIESCYGNTPEAIKSSPDFGRIVTEGNYLKIKSWLLRDIGQGARVIHGGNFDDHE
                     KYIEPTLITNVNCESALMQNEIFGPVLPILEYSTIDEALDFVKSLPKPLASYIFSKSS
                     SLQEYVLANTTSGGTTINDCLLHNFNAHLPFGGVNNSGMGKGRGVYGFYEFSNARGVL
                     NAKSYVRMPELLSAPYTKMKYKIADILLRWF"
     gene            complement(5306..>7325)
                     /locus_tag="PLE23_07290"
     CDS             complement(5306..>7325)
                     /locus_tag="PLE23_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83104.1"
                     /translation="NLPWHNQCNYTIPGFPYNVLASEDDGDGYWEHIQYVKVIDKTKP
                     TVECKDTTICSYGACEENVTLVGKASDGCDTAGLHLNYAYKVDLFNDGNYEIVSPANV
                     NSQTFNATVPFGRHRITWTIADGCGNFTTCSYIFWVKDCKKPTPVCHFLSAPTMPVQK
                     VVTIWATDFEAGSSFDNCCAYEDLVFRIVKTGESDHQTPPTATSVTFDCNELGSQPVE
                     LWAGDCGYDANGDGTISDDERNWDYCNTTILISDNDNVCGPGGFAAVAGTVETETGKM
                     VSGVNLIAVTGGSNQTTSNNGQFSFTLPTGQSYTIVPEKNVNPLNGVNTLDLVYMTNH
                     ILGKKALTSGYKKIAADINKDKKITTGDLVQLRQLILHITNEFPANTSWRFVEKSYSF
                     TTGNEQAENFPEVKVINNLASGVNANFIGVKVGDVTNDANPTNATASSEVRNGGTLTF
                     GVADQNLVAGQEYKVNFTAKDFTNILGYQYTIGYDVDALEFVGLEGKAADISADNFGL
                     TNLERGKITTSWNGKSATTLSADETMFTVTFKATKSGKLSKAINVNSSLTPAVAFNSN
                     EETMDVALEFNSNNGTVAASNFELLQNNPNPFKESTTISFILPAAGKATLSIYTVDGK
                     VVKTIQGDFAQGMNNVVINRSEISAAGVLYYQLDTDTDSAVRKMIIIE"
BASE COUNT         2228 a         1497 c         1490 g         2110 t
ORIGIN      
        1 gtcttgaagt tcgaagaaag agaaggaaga agacccagaa tacttgtagc caaactcggt
       61 caagacggac acgaccgcgg agcaaaggtc atcgctacag catttgccga cctgggcttt
      121 gatgttgaca ttgggccttt gttccagact ccgcaggaag cagccagaca agccgtagaa
      181 aatgatgtcc atatccttgg catttcttca ctagccgctg ggcacaaaac actcgtacct
      241 gaaaccatcg aagctctgaa aaatctcgga cgtgaagata ttttggtggt agtaggtggt
      301 gtcatacctc agcaggatta cgactatctt tttgaaaaag gcgttgcagg tattttcgga
      361 ccaggaactg tcatttccaa agcagcatca ggtattctga aactttttct ggaaaattga
      421 ttttgagagc attcgtctca aattttctga agaacaaaaa tatctatttc ggatgttgcc
      481 cccttgttgg taacagttat tgtatgccgc tgattctgat attcctgcat tttcggtaat
      541 tcgaaaaatc tgccggtcgc acttcttttc gggtcagtaa gaataattat tccacccgga
      601 acaagcattt tgtcgaaaaa tatactcaat gcactgtggt tcattttgtc atatagaaca
      661 tctgcaccga tgattatttc aaatgtttca ccatcaggtg gaaaatgcca gtcgagtaac
      721 ctgacgttgg gaggcgtgtc aaaatttaac cgccagttga tttttgctaa gtcaaccgcc
      781 tcttgcagat aatctgaaat tgtcacttga gcatttgaca aatttgcacc aatgcctgca
      841 agaccaagac cacacccgat ttcgagcaat cgctttcctt ctgataaatg ccggttgttg
      901 agcaaaaatt ctgccattgc aatggaagca ggccataact ctgcccagta aggtatccgt
      961 tcattgacca catcatcatg gttattacct ttttcaagca tttggtcaaa aagtgtctca
     1021 ggatgcaata cagtgcgcag ttttattaaa accccggcta tagtcaatgt aagtacggtg
     1081 gtttcaaatt gatattttcc tgacattttt tatttcataa agctggtcaa aattaataaa
     1141 attcaacaac tctcgttttc aaattgcgta taacacgtga ttgatgtaga gtttattaac
     1201 tttgcagcca tatttcattc aaccgattaa gtcaaattta tgataaacat tacttttccg
     1261 gatggcaatg tcagacagta cgaaacaggt actacacctt tgtccatcgc aaaatcaatc
     1321 agtgaaggtc ttgccaaaag gattctggtt gcaagcatca atggtcagat ctgggacatg
     1381 agccgccctg tatatgaaga ttcaaacatc aaactactgt cttgggatga taccgacggc
     1441 aaatcaacat tctggcactc ttcggcacac ctgatggcag aagcagttga ggctatgttt
     1501 ccgggagtta aattttgggt aggcccccct attgacaagg gcttttacta cgatatggac
     1561 cttggtgacg ctcagataac tgaagatgat ttggtcaagt tggagaaaaa aatgacagaa
     1621 cttgccaaac agaacaatct ttatacccga caggaggttt ctaaagcgga tgccgtagca
     1681 tatttcaccg aaaaaggtga tgaatacaaa ctcgatttgc tcgaaaatct ggaagacgga
     1741 gaaattacct actataccca gggcaatttt accgatctgt gccgtgggcc acacatccca
     1801 cacaccggtc ttatcaaatc tatcaaactg accaatatat cgggagccta ctggaagggt
     1861 gacgaaaaaa ataaagtact gacaagagtt tatggcgtga cattccccaa ccagcaggaa
     1921 atggatgagt acttgcacat gctagaggaa gcaaagaaaa gagaccaccg caaactcggc
     1981 aaagagctcg gaatatttac atttgatgac gatgttgggc caggccttcc gctctggatg
     2041 cctaatggta ccatcatcat cgaagaactc gaaaaactgg ccaaagaaac agaagaggca
     2101 gccggataca aacgcgttgt cacaccacac attgccaaag agagcatgta cctgacaagt
     2161 ggccacctgc cttattatca ggacagtatg tatccaccaa tggaaatgga tggtacaaaa
     2221 tattacctaa aggcaatgaa ctgtccgcat catcacaaaa tattcgacgc cgagccacgc
     2281 tcctaccgcg acctgccact ccgacttgca gaatacggca cttgttaccg atatgagcaa
     2341 agtggcgagc tgtttggcct tatgcgtgta cgatgtctac acatgaatga tgcacacatt
     2401 tattgtacta aagaacagtt ctttgaagaa ttcagagccg tcaatgatat gtatctgaag
     2461 tatttcaaga tattcgggat agaaaaatac gtcatgcgtc tgtcattgca cgatcctgcc
     2521 aaactcggcc aaaaatacat caacgaacct gaactttggc tcgaaacaga agaaatggtg
     2581 cgtcaggtat tgaaagatac aggcactccg ttcgtagaag tagcagatga aggagctttc
     2641 tacggaccaa agatagacgt tcagatatgg agcgccatag gtcgcgaatt tacacttgct
     2701 accaatcagg ttgacttcgc tcaaggcaga agatttaatc tcagctatac gacaagcgat
     2761 aattcgcatg acactccact catcattcac agagcaccgc ttggtacaca tgaaaggttc
     2821 atcggattcc tgcttgaaca ttatgcagga agattccctg tatggctttc tccggtacaa
     2881 gtaaaagtat taccgatcag tgataaagta gcagattttt cgaaaaatgt tttccagtcg
     2941 ctcaaatcag ccggtataag agtagaaatc gacaaccggg tagaaagtat tggtaaaaaa
     3001 attcgcgaag ccgaactaat gcgtgtacct tatatgctta ttgtaggtga aaaagaagct
     3061 gctgaaggga aagtatcggt tcgtaaacaa gctgaaggtg accttggcca gcagacgatt
     3121 gcagaatttg tagccaacat gcgcgagtgg tcaaatagac aatgacgaaa acagatgcct
     3181 atttagtata tttttgttaa aatttttata aaaacattta acaaatttga ttattacggt
     3241 actcttgtgt aaatttgtgg tatgaaacga attctactta gtatttttct tgtactctct
     3301 atggtgttct gtgcattttc acagtcatca tcagatggtt ttggtagttc agcttcatat
     3361 aacgaaaata ttaaagtatc tgtttatcca aatcctgctt cagattacat taatattcaa
     3421 gacaaaacaa atacaattac cagcatcaat gtttttgacc tggcaggtag aaagattaaa
     3481 actttcaaaa ctgaaggaat acagatgcta agcatagttg atttgccaaa aggtatttat
     3541 ctcatgcagt ttttggacaa aaaagaaaaa ttagtcacca cacagcgtct aagtaaaaaa
     3601 taaaaaatgt aaaaagcctt caaatcctga aggctttttt tatttacaca acatccgttc
     3661 atctaaaacc atctcagtag aatatcggca atcttatatt tcattttggt ataaggggca
     3721 gacagcaact ccggcatcct tacataggat tttgcgttta gtacacctct cgcatttgaa
     3781 aactcgtaga atccataaac acctcgccct ttacccattc cactattatt tacaccacca
     3841 aaaggtaaat gtgcattgaa attatgcaat agacaatcat tgatggttgt accaccggaa
     3901 gtggtattgg ctaatacata ttcctgcaaa gagcttgatt tagaaaatat ataactggca
     3961 agaggcttag gcaatgattt gacaaaatct aatgcttcat cgatagtact gtattcaagt
     4021 attggcaaca ccggaccaaa tatttcgttc tgcatcaaag ctgactcgca gtttacgttg
     4081 gtaataagtg ttggttcgat gtacttttca tgatcatcaa agttgcctcc gtgtatcact
     4141 cttgcaccct ggccaatatc acgtaataac catgatttta tttttaaata attgccttca
     4201 gtaactatac gtccaaaatc aggggaagat ttgattgcct caggcgtatt gccataacat
     4261 gattcgatcc tttcgattaa agcacttgat aattttgata ctacatcttt atgtacaaga
     4321 aggtaatccg gagcgataca tgtctgtccg gtatttaaaa attttgcaaa taccagactg
     4381 ttggctgtct ttgagatatc agcatcctga tgtactatgg ctggtgtttt cccaccaagt
     4441 tcaagcgtta cggatgctag attttcagca gctgccttca ttactaggcg gcctattgca
     4501 gggctacctg taaaaaagat atggtcaaaa ggaagtgaag taagataaga agcagtacga
     4561 gcatcacctt ctgctacata cacttcattc ggcggaaaaa cagcttctac aattttttta
     4621 atgactttca cggctgcagg cgtagcttca gaaggtttga gtatggcaca attacctgct
     4681 gcaatagcac tgaccagagg atcaaacaaa agattaaaag cataattcca tgggctaagt
     4741 atcagcacaa tgccttttgg ctcatgtata acatacgatt ttgccccaag taattccttc
     4801 ggtgttggcc ttctttccgg cttcatccaa tgtgataaat ttttcacaac aagattaata
     4861 tgcgaaataa ccgggagtaa ttctgtaata tctgtttcga gtgcaggttt tttaaatgaa
     4921 atattcattg cctctctcat ctcgttttgg tggctgagaa tactctcctt gagtagtttt
     4981 agctttagaa tgcgttgcga tgcgtttgat atttttacgc tatcaacatt ctgcttcatt
     5041 ttattgaata cagcgctgag atgcgtttca gcattcaaat attcataacc tggttcatta
     5101 acaatcatat ctatgcgtat agtttatgta aaaatacaaa ataagcaata agccaaagag
     5161 aaattgagtt ggtaatcttt gacatgttca atattgacaa aaaaaaagcc cccccccaaa
     5221 tttcttcgga gagaggccat cccaaaaacc gatttatagt ttatcgctag aaatcgatgt
     5281 tcggactatt caagatccaa aaggattact cgatgataat catctttctt actgctgaat
     5341 cagtgtcagt gtctaactgg tagtaaagaa caccggctgc agagatttcg cttctgttga
     5401 taactacgtt attcatacct tgagcgaagt caccctggat agtcttaaca actttaccat
     5461 ctactgtgta gatagaaagt gtagcttttc cagctgcagg taagataaag ctgattgttg
     5521 ttgattcctt gaatggatta ggattgttct gaagcagttc gaagttagaa gctgcaacag
     5581 taccgttgtt tgagttgaac tcaagggcaa catccattgt ctcttcgttg ctgttgaatg
     5641 caactgcagg agtcaagctt gaattaacat taatagcttt gctcaattta cctgatttag
     5701 tagctttgaa agtaactgta aacattgttt cgtcagcaga caaagtagta gctgatttac
     5761 cgttccagct ggtagtgatc tttcctctct caaggttagt caaaccaaag ttgtcagcgc
     5821 tgatgtcagc agccttacct tcaagtccaa cgaattcaag agcgtcaaca tcgtaaccaa
     5881 ttgtgtactg gtagccaagt atattagtga agtctttagc tgtaaagttc actttgtatt
     5941 cctgaccagc aactagattc tgatctgcaa caccaaatgt aagtgtacca ccatttctaa
     6001 cttcgctgct agctgtagca tttgttgggt tagcatcgtt tgtaacgtca ccaactttca
     6061 caccgatgaa gttagcatta acacctgatg caaggttgtt gataacctta acttcaggga
     6121 agttttctgc ttgctcgtta ccagtagtga agctgtatga tttctcaacg aatctccaag
     6181 aagtgttagc agggaattca ttagtaatgt gtagtatcaa ttgtctcaac tgtacaaggt
     6241 cacctgtagt gatcttctta tccttgttga tgtcagcagc aatcttcttg taaccagaag
     6301 tcaacgcttt cttaccaagg atgtggttag tcatgtaaac aaggtcaagt gtgttaacac
     6361 cgttaagtgg gttaacattc ttctcaggaa cgatagtgta agactgacct gtaggaagag
     6421 tgaagctgaa ttgaccgttg ttgcttgtag tctgatttga accaccagtt acagcaatta
     6481 ggttaacacc agaaaccatc ttaccagttt cagtttctac tgtaccagct actgcagcaa
     6541 atccacctgg accacaaacg ttatcgttgt cagagatcaa gatagtagtg ttacagtaat
     6601 cccagtttct ctcatcgtca gagatagtac cgtcgccgtt tgcatcgtat ccacagtcac
     6661 cagcccatag ttcaacaggc tgagaaccaa gttcgttaca gtcaaaagtt acacttgtag
     6721 cagtaggagg agtctggtgg tcagattcac ctgtcttaac gattctgaat actaggtctt
     6781 cgtaagcaca gcagttatcg aatgatgaac cagcttcgaa gtcagtagcc cagatagtaa
     6841 caaccttctg tactggcatt gtaggagctg acaagaagtg acataccgga gtaggtttct
     6901 tacagtcttt aacccagaag atgtatgaac aagtagtaaa gttaccacaa ccgtcagcta
     6961 ttgtccaagt gattctgtga cgaccgaaag gaacagtagc attgaaagtc tgagagttaa
     7021 cgttagcagg gctaacgatt tcgtagttac catcgttgaa caagtcaact ttgtaagcgt
     7081 agttaaggtg taggccagct gtatcacatc cgtcagatgc tttaccaact agagtaacgt
     7141 tttcttcgca tgcaccgtag ctgcagatag tagtatcttt acattcaaca gtaggcttag
     7201 ttttgtcgat aactttcaca tactggatgt gctcccagta accgtcacca tcatcttctg
     7261 atgcaagaac gttgtaaggg aatccaggga tagtgtagtt acactggttg tgccatggaa
     7321 ggtta
//
LOCUS       DAOQQY010000129         7289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4138_length_7289_cov_5.932817, whole genome shotgun sequence.
ACCESSION   DAOQQY010000129 DAOQQY010000000
VERSION     DAOQQY010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7289
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4138_length_7289_cov_5.932817"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1192
                     /locus_tag="PLE23_07295"
     CDS             <1..1192
                     /locus_tag="PLE23_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83105.1"
                     /translation="LLLMLGLLYYYLISRRLKLSAELEFLGLAALFWCYPVLKMPFFN
                     PIITDAAAFTMGLMVTYHFLCNQRIVNVLLILAGSFIFPTFILFGLLLLFPAEEFKAN
                     TSPKRMERLVLPALFLIAFVVVYFGFQNQFADTYSNINPTNRKLLFISVLLALGYMYQ
                     IGAFLPDFSNFKDALSKVKWIYLVPIVLIILFIKYMTATYTSPNPENIGGGRYLINIV
                     KQAVANPGVFLVSHIIYFGWLPLMLIFAGKEMVNAIRSLGYGVAILLAGVGFMSLGSE
                     SRQLINFYPVFVVAALLALQKKQALKPWVLALFGVSSLILSRFWYSITGYGDLGVNLT
                     EWPAQRYFQVFGPWMSNSTYAMNLFICLIAVSVILILQKTGNLLDPIPVKSVVQTKPT
                     NKRK"
     gene            1198..2166
                     /locus_tag="PLE23_07300"
     CDS             1198..2166
                     /locus_tag="PLE23_07300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX83106.1"
                     /translation="MPKLSIIMSCYYNGPNIPVTTKRLIDNEMNFPQDLDIEYVVIDD
                     GSKDDTFEQLCNFRNAYPHKVKVIKLAANVGSYNALLAGMAYATGDCCSIVAADLQDP
                     PELIPVMYGHWEKGYKLVIGQRENREDSWSDTLFSNIFNKMIRKFALPSIPKGGFDFV
                     LFDKQLKDEVVRMDEGNSNCMYLLAWQGFPYVTVPYTRQKRQIGKSRWTLKKKIKLFI
                     DSFVSFSFFPLRIISTAGIILGLLAILYAAFILIARITGHINVQGWSTLMIVFLFVSS
                     FQMIALGIIGEYVWRNLDAARKRPNYIIDTLLSNEEIDGDQSQIPH"
     gene            complement(2188..2988)
                     /locus_tag="PLE23_07305"
     CDS             complement(2188..2988)
                     /locus_tag="PLE23_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX83107.1"
                     /translation="MHIFRYGNFTVFRSRLYMTNSTVVDMGRSVLICDPSWLPDEIIA
                     ISEFVQNKYKDYQKYLFISHFDFDHLWGWPVFNDAQIICPDPNIFAEQAINGLMEWIE
                     WDENHYIIRPYKATLPENILLPLTSDEIFMENESDIHFFSVPGHTVDSFAAFLPKQNI
                     LLAGDYLSDVEIPWIGTNPEEYAHSLDVFSRIITEHKPAYVIPGHGDVHHSYRDALKI
                     IEDAQKYINCLIKGKCPKGFTEKYISSFPFEKASETIHEQNKKISRLK"
     gene            complement(2994..3584)
                     /locus_tag="PLE23_07310"
     CDS             complement(2994..3584)
                     /locus_tag="PLE23_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83108.1"
                     /translation="MNRYLILAFGLLILASCSEEKTTLPKPRAYPKVVFPVHAYQTFS
                     EKYCPMSFEYPAYAQIVQDTLYFNEKPENPCWFNVYIPQFNCYLYCTYTDLQGKKKFN
                     TVVNDAFNLAYKHDQRANYISDTKFTNENGLGGMQFEMTGPAATPFQFFITDSTTHFM
                     RGALYFNTQVRPDSLMPVYDFVKIDVDRLIKTFKFK"
     gene            complement(3581..4522)
                     /locus_tag="PLE23_07315"
     CDS             complement(3581..4522)
                     /locus_tag="PLE23_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMX83109.1"
                     /translation="MEADPAHLCQILDKAFITIQSSGLYSVMLTMLLGAVTGKKPFIK
                     AIERLFRMFGDKEEMTQNIAVRPSTEQDNIQEKSIRKGIERFGDVMVSQIMKARIDVV
                     HIDIHASFTELIKTFRDSGYSRIPVVDGSFDKIVGIMNVKDLLHFFDHNNHEQWEQVM
                     SKEVLYVPETKYINHMLQVFQEEKKHMAIVVDEYGMCTGLITMEDIMEEIFGEISDES
                     DINEEIVYRKIDNNSYLFLGKTMIVDLCKIMDLDHETFDDLRGNAETVAGLILENLER
                     VPAKGESIELAGINFTIQAADKRRILKIIVNKLNPES"
     gene            complement(4578..5021)
                     /gene="ssb"
                     /locus_tag="PLE23_07320"
     CDS             complement(4578..5021)
                     /gene="ssb"
                     /locus_tag="PLE23_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMX83110.1"
                     /translation="MINKVILIGNLGRDAEIRNLPNGSKVARFPIATNENYKDKNGDW
                     QTITDWHDIVAWRALADRAERDCKKGNTVFVEGKLRRRKYTDQNGVERISVEVEAENI
                     RVLDKKETAGHDEARSGTRPVSEHATESGYSSPFDESSQSDDLPF"
     gene            complement(5031..6089)
                     /gene="mutY"
                     /locus_tag="PLE23_07325"
     CDS             complement(5031..6089)
                     /gene="mutY"
                     /locus_tag="PLE23_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HMX83111.1"
                     /translation="MHNEDPNAVFTGLLLHWHENNPRPMPWKETLDPYRIWISEVILQ
                     QTRVHQGWDYYLAFIKKFPDNKTLAQSSLADVLKAWEGLGYYTRARNLHKAAKKIEDE
                     LGGKFPQLYKQILSLPGIGPYTAAAVASFAFNLPNAVVDGNVIRVLSRYFGLDGNGDV
                     GILKKEISEKADKLIQLSPSSAFNQAIMNFGALQCIPHKPDCTKCQMNKACFAFNHMR
                     TNDLPPKKKKILRKNRFFIYCLIHHKKGIFIEQRTNRDIWQHLYQLPCFETDENWSTD
                     NLPVFLKEKMSKVSLSEKIFHQTLTHQNIFARIIVLHTDEPLETKTDTLRYSEPNNLH
                     KFAFPKLVLNILQQQAII"
     gene            complement(6197..6733)
                     /locus_tag="PLE23_07330"
     CDS             complement(6197..6733)
                     /locus_tag="PLE23_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMX83112.1"
                     /translation="MSSVEFYNKFNALTAVLQAFSYNLTKNLEDSRDLYQETAYRALS
                     NREKFITGTNFKAWIFTIMKNIFINNYRKKVKANTIMDSTDNQFYLNSGSIATINEGD
                     NNIMMEELHAMIDSLDDSIRIPFEMHYEGFKYQEIADELNLPLGTVKSRIFFARKELK
                     DLIYRKYDKIASAYEMVN"
     gene            complement(6935..>7289)
                     /locus_tag="PLE23_07335"
     CDS             complement(6935..>7289)
                     /locus_tag="PLE23_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMX83113.1"
                     /translation="QKEMLTSLELLDYVEKENERGSGIAKPFEIEFHRRTADSISIII
                     LTLIGTAVATRKVRGGMGLHLAIGIGLGAIYVFLSRFSITFAQNDSLPSVIGVWIPNL
                     FFIGVAIYLNSKAQK"
BASE COUNT         1894 a         1427 c         1574 g         2394 t
ORIGIN      
        1 gctgctgctc atgcttggtt tactgtatta ttatctgatc agcaggcggc tgaaactgtc
       61 ggcagaactc gaatttcttg gtctcgctgc cttgttctgg tgctatccgg tgctaaaaat
      121 gccgtttttc aacccaatca tcacggatgc ggccgccttt acgatggggc tgatggtcac
      181 ctaccatttt ttgtgcaatc aacgcattgt caatgtcttg ctgatacttg caggatcctt
      241 catttttccg acatttatac ttttcggttt attactgttg tttccggccg aggaatttaa
      301 ggcaaatacc agtccgaagc gaatggagcg gcttgtactt cctgcgttgt ttctcatagc
      361 atttgtagtt gtatattttg ggtttcaaaa ccaatttgca gatacctatt ccaatattaa
      421 tccgacgaac aggaagctgt tgtttatttc tgttttgctc gcacttggct atatgtatca
      481 gattggcgca ttcttgcctg atttcagtaa ttttaaagat gctttgtcta aggtgaaatg
      541 gatttatctg gtaccgatag tgttgattat tcttttcatc aaatacatga cagccaccta
      601 cacctcaccg aatccggaga atattggtgg tgggcgatat ctcatcaata tcgtgaaaca
      661 ggctgtcgcc aatccgggcg ttttcctggt ttcgcacatc atctatttcg gttggcttcc
      721 gctgatgctc atttttgccg gtaaagaaat ggtgaatgcc atccggtcgc ttggatacgg
      781 tgtggcaata ctcttagcag gcgtcggatt catgtcgctt ggtagcgaga gcagacagct
      841 gatcaatttc tatcccgtgt ttgtggttgc ggctttgctt gcattgcaga aaaagcaggc
      901 tctcaagccg tgggtgctcg ccttattcgg tgtcagttca cttattttgt cgagattctg
      961 gtacagcatc accggctacg gagatttggg tgtgaatctg accgaatggc cggcacagcg
     1021 atattttcag gtattcggcc catggatgag caactcgacc tatgccatga atctgtttat
     1081 ctgcctgata gcggtgtccg taatccttat tttacaaaaa accggcaatc tgctcgaccc
     1141 gataccggtg aagtcggttg ttcaaacaaa accaactaat aaacgtaaat agaccagatg
     1201 ccaaagttgt cgatcatcat gtcgtgttat tataatgggc cgaatattcc ggtgaccacc
     1261 aagcggctta ttgacaacga gatgaatttt ccacaggatc tcgatatcga gtatgtggtt
     1321 attgacgatg gctctaaaga cgataccttt gagcaacttt gcaatttccg gaatgcatat
     1381 ccgcacaagg tgaaggtcat taagctggct gccaatgtgg gttcgtacaa tgctttgctt
     1441 gccggaatgg cttatgccac gggtgactgc tgctcaatag tggctgcaga cctgcaggat
     1501 ccgccggaat tgattcctgt gatgtatggg cactgggaga aaggctacaa actggtaatc
     1561 ggacaacggg aaaaccgaga agattcctgg tccgatacct tgttttcaaa tattttcaac
     1621 aaaatgatac ggaaattcgc attgccgtcc attcctaagg gcggatttga ctttgtactg
     1681 ttcgacaagc agctcaaaga cgaggtggtg cgtatggacg aaggcaattc caactgcatg
     1741 tacctgctcg cctggcaggg atttccgtac gtgaccgttc cttacacgcg tcaaaaaagg
     1801 cagatcggca agtcgcgctg gacgctgaag aagaagataa agctgttcat cgacagtttc
     1861 gtgtcgtttt cgttttttcc gctgcgcatc atttccactg ccggcattat cctgggcttg
     1921 cttgcgattc tgtatgcagc attcatcttg attgcaagaa tcaccgggca catcaacgta
     1981 caaggctggt ccacgctgat gattgtgttt ttgtttgtgt cttcgttcca gatgatagca
     2041 ttaggcatca ttggtgaata cgtctggcga aacctcgatg cagccaggaa acgaccgaat
     2101 tacatcatag atacgctatt gagcaatgaa gagatagatg gtgaccaaag ccagataccg
     2161 cattaggttc tttattgcaa ataattttta cttcaaacgg ctaatttttt tattttgctc
     2221 atgaatggtt tcggatgctt tctcaaaagg aaaagatgaa atgtattttt ctgtaaatcc
     2281 tttaggacat ttgcctttta taaggcagtt aatgtacttt tgggcatctt cgatgatttt
     2341 tagtgcatct ctgtacgaat gatgtacatc gccatgtccg ggaatgacgt atgctggttt
     2401 gtgttcggtg atgattcgtg aaaaaacgtc aagactgtgt gcgtattctt cagggtttgt
     2461 acctatccaa gggatttcga catcgctcag gtaatcgcct gccagcagga tgttttgttt
     2521 tggtaagaag gcagcaaaac tatcaactgt atgacccggt acggaaaaga agtgaatatc
     2581 gctttcattt tccatgaata tttcatcaga agtaagaggc agaagtatat tttcgggcaa
     2641 tgttgccttg taaggtctta ttatgtagtg attttcgtcc cattcaatcc attccattaa
     2701 tccatttatt gcctgttctg cgaaaatatt aggatcaggg caaatgattt gtgcgtcatt
     2761 aaagaccggc cagccccata ggtggtcgaa atcgaagtgt gaaatgaaaa gatatttttg
     2821 atagtcctta tatttgtttt ggacaaattc gcttattgct attatctcat caggtaacca
     2881 agatgggtca cagattaaca ctgatctgcc catatccaca actgtcgaat tggtcatgta
     2941 caacctgctt ctgaatacag taaaattccc gtatctgaag atgtgcattc cggttattta
     3001 aacttgaatg ttttaataag cctgtccaca tcaatcttaa caaaatcata taccggcatg
     3061 agcgagtcgg gtcttacctg tgtattgaaa taaagagcac ctcgcatgaa gtgagtagta
     3121 gaatcagtaa taaaaaactg gaatggcgta gcagccgggc ctgtcatttc gaactgcata
     3181 ccacccaggc cgttttcgtt ggtgaatttt gtgtcgctga tgtagttggc acgttgatca
     3241 tgcttgtaag cgagattgaa tgcgtcattt acgacggtat taaatttttt ctttccttga
     3301 agatctgtat aagtacaata aagatagcag ttaaactgtg gaatatagac attgaaccag
     3361 caaggatttt cgggtttttc attaaagtaa agtgtgtctt gtacaatctg cgcataagcc
     3421 gggtattcaa agctcatggg gcaatatttt tctgaaaatg tttggtaggc atgtacagga
     3481 aacaccacct ttgggtaggc tcgtggtttt ggaagggttg ttttctcttc actacacgaa
     3541 gccaatatta ataatccgaa tgcgagtatc aggtaacggt tcaactttcc gggtttaatt
     3601 tgttaacaat gattttcaga atgcgtcttt tgtcggctgc ctgaatggta aaattgatac
     3661 ctgcaagctc tatcgactca cccttggccg gtacacgttc aagattttcg agaatgagtc
     3721 cggctaccgt ttcggcattt cctcttaggt catcaaatgt ttcgtggtca aggtccatga
     3781 ttttgcacaa gtccacgatc attgtcttac ccaaaaagag gtagctatta ttgtctattt
     3841 ttcggtaaac gatttcttcg ttaatatcgg actcgtcact tatttcgcca aatatttctt
     3901 ccatgatgtc ttccatggtt attagacctg tacacatgcc atattcgtca accacgatgg
     3961 ccatgtgctt cttttcttct tgaaatacct gtaacatgtg gttgatgtat tttgtttcag
     4021 gcacatagag cacttctttt gacatgactt gctcccattg ttcgtggttg ttatgatcaa
     4081 aaaagtgaag aagatccttt acgttcataa tacccacaat tttatcaaac gaaccatcta
     4141 ctaccggtat cctagaataa ccggagtcgc ggaatgtctt gatgagttcg gtgaaggatg
     4201 cgtgtatgtc aatatgtacg acatctattc tggccttcat aatttggctt accattacgt
     4261 ctccaaagcg ttctattcct ttgcgtattg atttttcctg aatgttatct tgttctgttg
     4321 aaggtcggac ggcaatgttt tgagtcattt cctctttgtc gccaaacata cggaataatc
     4381 gttcgatggc cttgataaat ggttttttgc cggtgacggc tcccaatagc atggtaagca
     4441 tgactgagta aaggcctgat gattgaattg taataaatgc tttgtcaaga atctgacata
     4501 aatgagcggg gtctgcttcc aaggtaatga atgtattgat taactgaata ttacggaatc
     4561 atgaaaatat tgttcaatta aaatgggaga tcatctgatt gagatgattc gtcaaagggg
     4621 ctgctgtatc ctgattcggt tgcgtgttcc gacactggcc tggtacccga tcttgcttcg
     4681 tcatggccgg cagtttcttt tttatccaac acacggatgt tttcagcttc tacttctaca
     4741 gatatccttt ctacaccatt ctggtcggta tattttcttc ttcgcaattt gccttctaca
     4801 aatactgtat tacctttctt acagtctctt tcagcacgat cggcaagagc tcgccatgct
     4861 acaatatcgt gccaatccgt gatggtttgc cagtcaccgt ttttgtcttt atagttttcg
     4921 ttggtggcta tcgggaaacg tgctaccttt gatccgttag gcaaatttct tatttcggca
     4981 tctctgccca ggtttcctat cagtattact ttgttgatca tgattgtgtt ttaaattatt
     5041 gcttgttgtt gtaatatgtt tagtaccagt ttaggaaacg caaatttatg caaattattt
     5101 ggttctgaat atcgcaaggt atcagttttc gtttcaagtg gctcatctgt atgtaagaca
     5161 ataatgcggg cgaaaatgtt ctgatgagta agtgtttggt gaaatatttt ttcagacaag
     5221 gagactttgg acattttttc cttcaaaaag actggcagat tgtcggttga ccagttttca
     5281 tctgtttcga aacatggcag ttggtaaagg tgttgccaga tgtcgcggtt agttctttgt
     5341 tcgatgaata tacctttttt atgatgtata aggcaataaa tgaagaagcg gtttttacgt
     5401 aaaattttct ttttcttagg tggcaaatca tttgtacgca tgtgattgaa tgcaaaacac
     5461 gccttgttca tctggcattt ggtacaatcc ggtttatgag gtatgcattg caatgcacca
     5521 aaattcataa tggcttggtt gaatgctgaa gaagggctga gctgtataag tttgtctgct
     5581 ttttcgctta tttctttctt taggattcct acatcaccat tgccatcaag accgaagtac
     5641 ctagacaata cccttatgac attgccatca actaccgcat ttggcaggtt gaatgcaaat
     5701 gatgctaccg ctgcagcagt ataaggcccg ataccaggaa ggctcagtat ttgtttgtac
     5761 agttgtggaa atttaccacc caactcatct tcaatttttt ttgctgcctt atgcagattt
     5821 cgtgccctgg tataatatcc aagtccttcc caggccttta acacatccgc aagggatgat
     5881 tgtgccagag ttttgttatc tgggaatttt tttataaatg ccaggtaata atcccagccc
     5941 tggtgtacgc gtgtctgttg aagtattact tctgatatcc agatgcggta tgggtcaagg
     6001 gtctctttcc agggcatagg ccttggatta ttttcgtgcc aatgcagcag gagtcctgta
     6061 aatacagcgt ttgggtcttc attatgcatg agttgtgcgt attggtctgt aaaaatacat
     6121 tttcacacag acaaaaaatg gcaacaaatt tattttgctg ccattttaag aatatgaagg
     6181 attgacgaat tttcttttaa tttaccattt cgtatgcact tgctattttg tcgtactttc
     6241 tgtagatgag gtctttaagt tcttttctgg caaagaatat tctactcttg accgtaccga
     6301 gtggcaaatt aagctcatct gctatttcct gatatttgaa gccttcgtaa tgcatctcaa
     6361 agggaatgcg tatagaatca tcaagtgagt caatcatggc gtgcagttct tccatcataa
     6421 tgttgttgtc gccttcattg atggtagcaa tactgccact gttcaggtaa aactgattat
     6481 ccgtactgtc catgatggtg ttagccttca ctttcttgcg gtaattgttg atgaaaatgt
     6541 tcttcatgat ggtaaatatc catgccttga agttggtacc ggttataaat ttctctctgt
     6601 tgctgagagc tctatatgct gtttcctgat acaaatcgcg cgaatcttca agattttttg
     6661 taagattgta agaaaatgct tgtagcactg ccgtcagggc attgaactta ttatagaatt
     6721 cgactgatga cataatgttg tgtttttatt tgttgaacca aagttacttc aagcttaaac
     6781 aaaaaacaag cttgtcagaa aaaatattta gataaattct aattaaggtt gcaaaatatt
     6841 gaaaatcaga taattaaatt ttcttaaaat tttgttaaac aattttaact ttcaatttat
     6901 tttgaaagtt ttataaaaaa ttttattaga tgccttactt ctgggccttt gaattgagat
     6961 aaatggctac tccgataaaa aatagattgg gtatccatac gccgataaca gaaggcagtg
     7021 aatcgttttg tgcaaatgtt atcgaaaatc tggaaaggaa tacataaatg gcacccaagc
     7081 caataccaat agcaagatgc agacccattc cgcctctcac ttttcgggtg gcaacggctg
     7141 tgcctatgag cgtaaggata atgatagaaa tgctgtcagc agtacgacgg tgaaactcta
     7201 tttcgaatgg tttggcaatt ccggatcctc gttcgttttc tttttcaaca tagtccaaaa
     7261 gctcaagtga agtaagcatt tctttctgg
//
LOCUS       DAOQQY010000130         7250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4171_length_7250_cov_7.500208, whole genome shotgun sequence.
ACCESSION   DAOQQY010000130 DAOQQY010000000
VERSION     DAOQQY010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4171_length_7250_cov_7.500208"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..2792
                     /gene="infB"
                     /locus_tag="PLE23_07340"
     CDS             81..2792
                     /gene="infB"
                     /locus_tag="PLE23_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMX83114.1"
                     /translation="MAQRLIQVAKELNVGLGSLVEHLHKKGFDIDGKPTSMVDEDMLA
                     ELYREFQKSAAIKEEADKISFGIGRPTPPPVTPEPPKPVVATPEPKEEKPEEPKPKVI
                     KPNIEDYKPQLKVVGKIDLEPKKTEKTEKEKPTVPEPKAVEEVKPPVEIQPEKTESAT
                     VPTPAVEPVPEQDDVIRAEAPTLKGLKILGKIDTDKFTSPANKRKEAAKDKPENKPNP
                     SQAPNNSADDKKKKRKRKKVQQPPGITDKNTVGANKNKAPETPKEVSKKEIDDKIKAT
                     MARLSAGGKKKRQKIRRDKRETFREKQEMLELENANEKLQLTEFVTVSELASLLDVPF
                     TEVITTCMNLGVIVSINQRLDAEIIELVANEFGSEVEFISAEEQLEEDTEEEDDVDDL
                     KPRAPIVTVMGHVDHGKTSLLDYIRSEKVAAGEAGGITQHVGAYEVSIGEDNKKITFL
                     DTPGHEAFTAMRARGAKITDIVVIVIAADDNIMPQTREAISHAQAAGVPMVFAINKVD
                     KPAANPEKIKESLASMNLLVEEWGGQYQSQDISAKTGLGIDLLLEKILIQAEVLDLKA
                     NPDKRASGTTIEASLEKGKGYVTKLLVQNGTLRIGDSIVAGEFAGKVKAMFNERGKKL
                     KEAGPSTPVLILGLNGAPQAGEKFRNTETDAEARQIATKRAQISREQANRANKRISLE
                     EIGRRLALGNFKELKIIVKGDVDGSVEALSDALIKQSIESVQVNVIHKAVGQIIESDV
                     LLASASDAIIIGFQVRPSATARKLAEKEGVEIKLYSIIYNAIEEVRAAMEGMLEPTLK
                     ENIVGQIEVREVYKISKVGTVAGCYVQEGKVLRSNYIRIIRDGIVVFPTKEGAKGEFS
                     SLKRFKDDVKEVRNGFECGLTIKNYNDIEVGDIIEVYEIVSEKQTLS"
     gene            complement(2897..5407)
                     /gene="bamA"
                     /locus_tag="PLE23_07345"
     CDS             complement(2897..5407)
                     /gene="bamA"
                     /locus_tag="PLE23_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HMX83115.1"
                     /translation="MKITLQIILVLLLSVIHVQAQVTPFPGIELAPGEYEIGGITVSG
                     ASYSDENALKIVSGLKVGSKIRVPGEDLGNAIRALWKLKLFDKIEISKEKTLGEVLFI
                     NIKVTERPRISKHSFSGVKKGSHEDLNKIVERFLPKGTMITENSKNNVAYGIKNYWRE
                     KGYLDTEVKVIEETDDSLLNSAKVLFAIDRHKKVKIQNITFEGNKSAKSSKLRKLLEN
                     TKRRRKLFASSKFIKKDYEADKEKIIQYYNTIGMRDARIISDSIWRGKNGHLNIHMKI
                     EEGNRYYFRNISWKGNSIHNTETLNKVLGITKGEVFNQELLDNRLRFSQDGRDISSLY
                     LDNGYLFFQVDPQERVIGNDSIDLDIKIYEGPQATIDRVIIKGNDRTNEHVIRRELYT
                     RPGDKFSRQDIIRSQRQIAALGYFNPETLGVNPITNPVKKTVDIEYTVEEKPSDQLEL
                     SAGWGGAGRGVIGTLGVSFNNFSIHNILKKESWNPLPQGDGQRLSLRAQSNGKYFQSY
                     NASFTEPWLGGKKPMSFTVGGFLTNLTNGQDKSSTSYSNLKIINGTVGLGTRLKWPDD
                     NFILNTELNIQSLQLTNYAVPLSNGLIIDNGKFNNWSLRFTLARNSIGDPTFPREGSQ
                     IEFIGQFTPPYSLFNPSRNYADEPADKKYKLLEYHKWRVNAVWYTTIVQNLVFKASAK
                     IGLLGHYNNKIGTSPFERFVLGGDGLSGSDAFFNGNDLIALRGYDVSQLPASNSGGGA
                     AFDKFTLELRYPISLNPSSTIYVLSFLEGGNSWASIRDFNPFDLKKSAGLGVRVFLPV
                     FGTIGFDYGLGFDKPEIKKGSGLAQYGRFSIILGFEPD"
     gene            complement(5466..6230)
                     /locus_tag="PLE23_07350"
     CDS             complement(5466..6230)
                     /locus_tag="PLE23_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HMX83116.1"
                     /translation="MKELKSQIVSNNIPVHVAVVMDGNGRWAKQHGKPRVFGHKNGVK
                     SIRDVSEAAAELGIKYLTLYAFSTENWNRPALEVNALMTLLVETIRKEVATLNKNNIR
                     LQAIGDISRLPEKSRKALAKAIEDTAQNNMMTMVLALNYSSKWELTKAVIDIAKQVKE
                     NKLDLGSIDESVITRHLATKDIPDPELLIRTSGELRLSNFLLWQLAYSELYFTDTYWP
                     DFNKEEFFKAIISYQSRERRFGKTSEQLISNTSNIN"
     gene            6395..>7250
                     /locus_tag="PLE23_07355"
     CDS             6395..>7250
                     /locus_tag="PLE23_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HMX83117.1"
                     /translation="MTYSLAIHGGAGTILRSQITQEDEVKYNHALHDALVAGNDVLIK
                     GGNAVEAVAASVVSLENCPLFNAGKGSVFSNEWKHEMDASIMDGNNLQAGAVSMLHNV
                     KNPVLLAKEIMQNSEHVYLCGEGAERYARQQGLSFEPDEYFFTHLRKEQLEEALRDNK
                     IMLDHRTAKDRKFGTVGAVARDIHGNIAAATSTGGMTNKKFNRIGDSPIIGAGTYANN
                     HTCAVSCTGHGEYFIRGVVAYDVSCLMEYKGMSLMQACEKVILEKQVKLGGEGGLIAV
                     DNAGNLALI"
BASE COUNT         2270 a         1497 c         1436 g         2047 t
ORIGIN      
        1 aataaagtat aagaaatgtg cggttcacta aaaaaaccgt acatttgcac aaatttttaa
       61 tagttaattt aaataattga atggctcaac gcttaattca ggtagcaaaa gaacttaatg
      121 tgggtttggg atcattggta gaacaccttc acaagaaagg tttcgatatt gatggcaagc
      181 ctacttctat ggtagatgaa gatatgctgg cggaattata ccgcgagttt caaaaatctg
      241 ctgccatcaa agaagaggca gacaagatta gctttggtat tggacgccca actccgcctc
      301 ctgtaacacc cgaacctcct aagcctgtag tggccactcc tgagccaaaa gaagaaaaac
      361 ctgaagaacc caaacccaag gtaataaagc caaatattga agactacaaa cctcaactga
      421 aggtagtcgg caaaatagac ctcgaaccaa agaagactga aaagactgaa aaagagaaac
      481 ccaccgtacc agagccaaaa gctgtagaag aagtaaaacc acccgtagaa atacaaccgg
      541 aaaaaacaga atctgctaca gtacctactc ctgctgtcga accggtacca gaacaggatg
      601 atgtcatcag agcagaggct cctacactca aagggcttaa aattttaggt aaaatagata
      661 ccgataagtt tacaagcccc gctaataaaa gaaaagaagc agccaaagat aaacctgaaa
      721 acaaaccaaa cccatcacag gcacccaata acagtgcaga tgataaaaag aaaaagcgaa
      781 agcgcaaaaa agtacaacag ccgccaggta ttactgataa aaataccgta ggcgctaata
      841 aaaataaagc accagagacg cccaaagaag tatcgaaaaa agaaatcgac gataagatta
      901 aggctacaat ggccaggctt tctgcaggtg gtaagaaaaa acgccagaaa ataagaagag
      961 ataaacgcga aacattccgt gaaaagcaag aaatgctcga actcgaaaat gcaaacgaaa
     1021 aattgcaatt gacagagttt gttactgttt ctgagcttgc cagtctgctc gatgttccct
     1081 tcacagaggt cattactact tgtatgaacc tcggtgttat cgtttccatc aaccagcgat
     1141 tagatgccga aattattgaa cttgtagcta atgaattcgg tagtgaagta gaatttatta
     1201 gtgctgaaga acagcttgaa gaggatacag aagaagagga cgatgttgac gatctaaaac
     1261 ctcgcgcacc tattgttaca gtgatggggc acgttgacca cggtaaaact tccttgcttg
     1321 actacatccg tagcgaaaaa gtagcagccg gagaggctgg tggtatcact cagcacgtgg
     1381 gcgcatacga agtaagtatc ggtgaagaca acaaaaaaat aactttcctc gatacacctg
     1441 gacacgaagc atttacagca atgagagcac gtggagccaa aattacagat atcgttgtaa
     1501 tagttattgc tgccgatgac aatattatgc ctcagacacg tgaagctata tcacacgccc
     1561 aagcagctgg tgtacctatg gtattcgcta ttaataaagt agataaacct gccgccaacc
     1621 ctgaaaaaat taaagaatca cttgcaagca tgaacttgct ggtagaggaa tggggaggcc
     1681 aataccaatc acaggatatt tctgcaaaaa caggtttggg tatcgatctg ctactggaga
     1741 aaatattgat acaggcagaa gtgcttgacc taaaggctaa tccggacaaa agagcttctg
     1801 gtactactat agaagcatca ctcgaaaaag gaaaaggtta tgtaaccaaa ttattggtac
     1861 aaaacggtac tctcagaata ggtgactcaa tcgttgcagg tgaattcgcc ggtaaggtca
     1921 aagccatgtt taacgaaaga ggcaaaaagc ttaaggaagc tggtccttca acacctgttc
     1981 ttatcctcgg gctgaatggt gcacctcagg caggtgaaaa attcagaaat actgaaactg
     2041 acgctgaagc cagacaaata gccactaaac gagcccaaat atcgcgcgaa caggcaaaca
     2101 gagccaataa gcgaataagc ctcgaagaaa taggaagacg tctcgcatta ggtaatttca
     2161 aagaactgaa aatcatcgtt aaaggtgacg ttgatggttc tgtagaagct ctctctgatg
     2221 cactgatcaa acaatcaata gaatccgtac aggttaacgt tatacacaaa gctgtaggcc
     2281 agattatcga atcggatgta ttgcttgctt cagcttctga cgccatcatc attggtttcc
     2341 aggtaagacc atctgctaca gctcgtaaac ttgccgaaaa agaaggtgta gaaataaaac
     2401 tatactccat catctacaat gctattgaag aagtcagagc agcaatggag ggtatgctcg
     2461 aaccaacatt gaaagaaaat attgtcggac agatagaagt aagagaagtt tacaaaatat
     2521 caaaagtagg cacagtagca ggttgctatg ttcaggaagg taaagtattg cgctccaact
     2581 atatacgtat catacgagat ggtatagtgg tattccctac caaagaaggt gcaaaaggcg
     2641 aattttcctc actcaagaga tttaaagatg atgtcaagga agtcagaaat ggctttgaat
     2701 gtggtcttac catcaaaaac tacaacgata tcgaagtagg tgacattata gaagtatatg
     2761 aaatcgtttc agaaaaacag acactgagct aagcactact ctgtttatac ttttatacaa
     2821 taaaaaaaag tgcatcaaga tttaatcctg ttgcactttt tttttattcg aaatccaatt
     2881 ggactcaata tttacattaa tcaggttcga aaccaaggat tatactgaac cttccatatt
     2941 gcgccaagcc cgaacctttc tttatttcgg gtttatcaaa gcctaatccg tagtcaaaac
     3001 cgatggtacc aaataccggc aggaacactc gcacaccaag accagccgat ttcttcaagt
     3061 caaacggatt gaagtcgcga atggatgccc aagagttacc accttcgaga aatgataaaa
     3121 cgtatatagt cgaactcgga ttgagcgata taggataacg tagttctaag gtaaatttgt
     3181 caaaagcagc tccaccaccc gaattactcg caggcagctg gcttacatcg tatccgcgta
     3241 gagctatgag gtcattaccg ttaaagaaag catcgcttcc tgaaagcccg tcaccaccca
     3301 gtacaaatct ctcaaaaggc gatgtaccta tcttattatt ataatgcccc aatagaccaa
     3361 tcttggcaga ggccttgaaa acaagatttt gaacaattgt tgtgtaccac accgcattta
     3421 cacgccattt atgatattca agcaatttgt atttcttatc agccggttcg tcagcataat
     3481 ttctacttgg gttaaacaaa ctgtaaggtg gcgtaaactg ccctatgaat tctatctgag
     3541 acccttcgcg cgggaatgtc gggtcgccaa tagagttacg agccagcgta aatctcagtg
     3601 accagttgtt aaatttacca ttatcaataa tcaatccgtt tgacaatggt acagcatagt
     3661 tggtcaactg taggctttga atgtttagtt cggtgttgag tataaagtta tcatccggcc
     3721 attttagtct ggtgcctagt cccactgtac cgttgattat cttcaggtta ctatagctcg
     3781 tactactctt gtcctgtccg tttgtaagat ttgtgagaaa accaccaaca gtaaaagaca
     3841 tcggtttctt tcctccaagc catggttcag taaatgatgc attgtacgac tgaaaatatt
     3901 tgccatttga ctgtgcacgg agcgataacc gttgtccgtc accttgaggc aatggattcc
     3961 agctttcttt ctttagtata ttatgtattg aaaaattatt gaacgacaca ccaagtgtac
     4021 ctatcactcc tcgtcctgct ccgccccatc cggctgacaa ttcaagctgg tcagaaggtt
     4081 tttcctctac agtatattca atatcaacgg ttttcttaac tggattggta atagggttga
     4141 cacccaatgt ttctggattg aaataaccga gtgcagctat ttgtctttgt gaacggataa
     4201 tatcctgacg actgaattta tcacccggac gagtgtacag ctcacgtctt atcacgtgtt
     4261 cattggtgcg gtcattgccc ttaataatga cacggtctat tgttgcctga ggaccttcat
     4321 atatttttat gtcgaggtct atcgaatcat tgcctatgac ccgttcttgc ggatctacct
     4381 gaaaaaacag ataaccattg tcaagataca gcgaacttat gtcccttcca tcttggctaa
     4441 agcgaagtct gttatcgagc agctcttgat tgaaaacctc accttttgtt atgccgagta
     4501 ctttgttgag cgtttcggta ttatgtattg aattgccttt ccagctaata ttcctgaaat
     4561 aatatctgtt gccttcttca atcttcatgt gtatattcag atgtccattt ttgcctcgcc
     4621 agatcgaatc gctgatgatt cgggcatctc tcataccgat ggtgttataa tattggataa
     4681 tcttttcctt atctgcttca taatctttct taataaattt tgatgaagca aataactttc
     4741 tccttctctt ggtattctca agcagcttcc tgagttttga agactttgca cttttgtttc
     4801 cttcaaaagt aatattctga atctttactt ttttgtgtct gtctatcgca aacagaactt
     4861 tagcagaatt taataaactg tcatcggttt cttcaataac cttcacttct gtatccagat
     4921 atcccttttc gcgccagtaa ttcttgatgc cgtaagctac attattcttc gaattctcgg
     4981 ttatcatggt tccttttggt aaaaatcttt ccactatttt gttcagatct tcatgacttc
     5041 ccttcttgac acccgaaaaa gaatgtttgg atatacgagg tcgctctgtt accttgatat
     5101 tgataaacaa aacttcacca agcgtctttt ctttgcttat ttctatttta tcaaataatt
     5161 tgagtttcca caatgcccga atggcatttc caaggtcttc gcccggtact cttattttcg
     5221 atcctacctt taggcctgat actattttta gggcattttc gtcagaataa gatgctcctg
     5281 atacagttat tcctcctatt tcatattcgc ctggtgcaag ttcgatacct ggaaatggtg
     5341 ttacctgagc ctgtacatgt atgaccgaca ataacaaaac caatattatc tgaagagtta
     5401 ttttcattga atgtatttta tcacttgcga atattaagaa cgaatttatt gttcatatcg
     5461 cttcgttaat ttatatttga agtattgctt attaattgtt cactggtttt tccgaatctt
     5521 ctttctctac tttggtaact gataattgcc ttgaaaaatt cttctttgtt aaaatctggc
     5581 caatatgtgt cagtaaaata taactcagaa taagccaact gccacagtag gaaattactc
     5641 agccggagtt caccgctggt acgtatcaat aattcaggat caggtatgtc tttcgttgcc
     5701 aaatgccttg taatgaccga ctcgtcaatg cttcctaggt caagtttatt ctcctttact
     5761 tgcttcgcaa tatctattac tgccttggta agctcccatt ttgagctgta attaagcgca
     5821 agtaccatag tcatcatatt attttgggca gtatcctcga tggctttagc caaagccttt
     5881 cttgattttt caggaagcct gctgatatcg cctatggcct gcaatctgat attgtttttg
     5941 tttagtgttg ccacttcctt acgaatggtt tctaccagta acgtcatcag tgcattgact
     6001 tccagtgcag gtcgattcca gttttctgtt gagaaagcgt acaatgtaag gtatttgata
     6061 ccgagttctg ctgctgcctc cgacacatca cggatagatt taacaccatt tttatgacca
     6121 aatactcgag gcttaccatg ttgctttgcc caacgaccat taccgtccat gacaactgcc
     6181 acatgaaccg gaatattatt tgaaactatc tgcgatttta attctttcaa aactttgttt
     6241 aagacgactg gcaaagttag ttattgtcta ttcaaataaa aaatcaataa caagtctgat
     6301 tttaaaatat tgcctaaaaa aagatgttaa tatttggttt atctttgtct gcttataaaa
     6361 ttcataagat ttaactaacc aaaaatataa aacaatgact tattcgctgg ccatacatgg
     6421 tggtgccggt accattttaa ggtcgcaaat cacccaggaa gatgaggtaa aatacaacca
     6481 tgcgctacat gacgctttgg tagcaggcaa tgatgtattg attaaaggcg gtaatgctgt
     6541 ggaggctgtt gccgcttctg ttgtttcact cgaaaactgc cccttgttca atgccggtaa
     6601 aggatctgta ttttctaatg aatggaaaca tgaaatggat gcgtctatca tggatggtaa
     6661 caatctgcag gctggtgcag taagtatgct tcacaatgta aaaaatccgg tattgctggc
     6721 caaagaaatc atgcaaaatt ctgagcacgt ctatctgtgt ggtgaaggag ccgaacgata
     6781 cgcccgccaa caaggactga gcttcgagcc tgatgaatat ttctttactc atttgcggaa
     6841 agaacaactc gaagaagcat tgcgcgacaa taaaatcatg cttgaccacc gaactgccaa
     6901 agaccgaaaa ttcggtactg taggtgctgt tgccagagat attcacggaa acatagccgc
     6961 tgccacttcc accggaggta tgactaataa aaaattcaac agaataggtg acagcccgat
     7021 tataggagca ggcacttatg ccaacaacca tacatgtgct gtcagttgta ccgggcatgg
     7081 cgaatatttc atccggggtg tagtagctta tgacgtttct tgcctgatgg aatacaaagg
     7141 aatgagcctc atgcaggcat gcgaaaaagt gattctggaa aaacaggtaa aacttggcgg
     7201 agaaggcgga ttaatagctg ttgacaatgc cggcaacctt gcacttatat
//
LOCUS       DAOQQY010000131         7244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4178_length_7244_cov_5.018918, whole genome shotgun sequence.
ACCESSION   DAOQQY010000131 DAOQQY010000000
VERSION     DAOQQY010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7244
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4178_length_7244_cov_5.018918"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLE23_07360"
     CDS             <1..524
                     /locus_tag="PLE23_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMX83118.1"
                     /translation="WGTSEWSAMEIMEAQMIARQNHLIGPVVEQPEYNMFRRDKVESE
                     FSKIYHTVGLGTTIWSPLASGILTGKYLDEFPQNTRMSLETVGRLKEKALTEAKLNII
                     RQLDILAKETGISLARLSIAWCLKNPNVSTVILGASKIAQLEETLTATAVLPLLTEEI
                     MLRIDEILGNKPK"
     gene            complement(536..1615)
                     /locus_tag="PLE23_07365"
     CDS             complement(536..1615)
                     /locus_tag="PLE23_07365"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HMX83119.1"
                     /translation="MRTPIAIELTKTSKLSSVDFNNIPFGRVFSDHMYVADYNGKEWI
                     NPRIVPFGHFMIHPANLALHYGQSIFEGMKAAKQHDGKPVLIRPEMHSRRLNASAVRM
                     CMPEFPEDMFLDALHTLLAIDREWIPTAEGSALYIRPYMFATGEYIGVQASDVYRMII
                     FSGPVGPYYNKPVKLLAEEKYVRAARGGVGEAKTSGNYAASLLPAKLAKEKGFDQVLW
                     LDAQEFKYVQEVGTMNIFFVIGDKIVTPSTDGAILRGITRESIIDLLKDQGYEVEKRP
                     VSIEELVEAYHNGSFKEAFGTGTAAWVAHVAEIQYKDTKMILPPVEERKIGQLAYDLL
                     KGVRLGTLPDKHNWVVPVRELQIPV"
     gene            1710..2153
                     /locus_tag="PLE23_07370"
     CDS             1710..2153
                     /locus_tag="PLE23_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83120.1"
                     /translation="MLKQNNISYLALFNDFDIFTRNNQFANQMPDRRFFFVYKSEDTP
                     YIGFLQDIIKATKINGDDYRIIELSENVLLRLGVFFENELCESIIFFGFTNEELGFPT
                     NFRKYKPFLLREKSFIIADSLSIIQPDKTLKSALWQSLKELFNIV"
     gene            2150..2728
                     /gene="rdgB"
                     /locus_tag="PLE23_07375"
     CDS             2150..2728
                     /gene="rdgB"
                     /locus_tag="PLE23_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HMX83121.1"
                     /translation="MKKIIFATGNMNKLNEVRQILHEQYQILSLEELDFHEELAEDGD
                     TFEDNARQKALFITEKFGLPCFAEDTGLEVDALNGAPGVYSARYAGTQRSHADNINLL
                     LKNLEGHTNRNARFKTIIALTFDKQIYYFEGTVNGRIAYQPSGNAGFGYDPIFIPDGY
                     DQSFGELPTEVKNQISHRAIAVEKLLRFLQGK"
     gene            complement(2757..4571)
                     /locus_tag="PLE23_07380"
     CDS             complement(2757..4571)
                     /locus_tag="PLE23_07380"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HMX83122.1"
                     /translation="MNNSFNRFFLLVILILSTALVQARDGYWQQYVHYTMDIDFDVSK
                     HQFKGQQKLILVNNSPDTLNQLFYHLYFNAFKPGSAMDVRSRNIPDPDPRVADRISKL
                     KKSEYGNLNVDRLTLNGKPVSFTTQETILQVFTEGQLILPGDTVILEMAFNGQVPLQI
                     RRSGRDNKEGIDYSMSQWYPKLCVYDEDGWHPNPYIGREFYGNWGDFDVNITIDKDYL
                     VAGTGIATDIDGKLLPDGKKKLWQFRAERVHDFMWAADRDYVRTSVTLTNGPVVYLYY
                     QDYPEYAESWKRLPHYLNEMFHLASSGYGKYPYPVFYIIQGGDGGMEYPMGTLVTGKR
                     SLASLVGVVSHELLHSWYQGVLGINESLYPWMDEGFTSYASDIITSKLLDPQGKNYPF
                     TPSYNSYFSVVKQGIEEPLCTHADHYNTNSAYGMASYSKGFVYLHQLSYVVGQKVLDK
                     ALLEFFRDWSFKHPKPNDFLRIVEKSSGMVLDWYNEYWINSTHTIDYGIKEVKTRRGK
                     YTVTLQRIGKVPMPLDVIVTYTDNTSECYNIPLVLMRGSKENDTKVEPYTVLKAWQWP
                     DTEYSFQFNMTKKIKKIEIDPSARMADVNKDNNVFIAE"
     gene            complement(4585..5400)
                     /locus_tag="PLE23_07385"
     CDS             complement(4585..5400)
                     /locus_tag="PLE23_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HMX83123.1"
                     /translation="MHWKSSYLRLFFSVIIIILCFIAGAYPDSFITEDNDQSRLFLML
                     LPFCTGLAGLYLVFRFLHHADFSKLFIPDGILKISVKRIFFGFFLWLAIQIFLEIIMF
                     MLNPDDYSFQYDLPVSWLWLVLVALLMIPAQTSFEELFVRSYLLQYFYKFFDKKFVAL
                     CSSSIFFAILHYSNPEIVEYGAGIMLFYYFLAGLGIGLISYFDNRMELALGMHASNNI
                     YAAVLVSYKKAAFDTQPLIEVSSMNVFASIALFLIGIFIFLLICKYNYKWVLP"
     gene            5538..6098
                     /locus_tag="PLE23_07390"
     CDS             5538..6098
                     /locus_tag="PLE23_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83124.1"
                     /translation="MNENNQKYLLIVGLIFTIALSRLVPHPWNFTPVTALFIFAGSFA
                     RKKMVNFLIPFSAVLLSDFLVNYFIYKTAGFTFFYPGAWMVYLAYLVIFLFNHTIHTV
                     SYPGIAAKSLISSIIFFLITNFAAWYASPMYAQNFGGLMTSYTAGLPFFGNGILGDLF
                     YSFILVAAYNMLYRSLLQRAEIRSQR"
     gene            6134..6283
                     /locus_tag="PLE23_07395"
     CDS             6134..6283
                     /locus_tag="PLE23_07395"
                     /inference="COORDINATES: protein motif:HMM:NF037605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5522 domain-containing protein"
                     /protein_id="HMX83125.1"
                     /translation="MEVTEDIDFYVENGYYVFTAHYLLRRGYCCQSGCRHCPYGFKKK
                     KAHTD"
     gene            complement(6368..6913)
                     /locus_tag="PLE23_07400"
     CDS             complement(6368..6913)
                     /locus_tag="PLE23_07400"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMX83126.1"
                     /translation="MKIYTKTGDSGETSLFGGKRVPKDHVRIEAYGTIDELNSFLGVL
                     YDNLEDENQRYLINSVQSNLFTIGSILANEGKTTDYIPDLRESAISVLEKAMDEMDEN
                     LPPLTNFILPGGNTVVSFCHIARTVCRRAERRMVALNHIEDVDPKLIKYLNRLSDYLF
                     VLSRKIAKDFNAEEISWKPGM"
     gene            complement(6932..>7244)
                     /locus_tag="PLE23_07405"
     CDS             complement(6932..>7244)
                     /locus_tag="PLE23_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX83127.1"
                     /translation="HKPNELSGGQRQRVAVARALVNDPAIILADEPTGNLDTKTSIEI
                     MALFEEIHSKGNTVVLVTHEPDIALYAHRIIRLRDGQLESDEQNSNIIRVVGNAKPLA
                     G"
BASE COUNT         2194 a         1446 c         1413 g         2191 t
ORIGIN      
        1 actggggcac ttcggaatgg tcagccatgg aaatcatgga agcacagatg atagcccgcc
       61 aaaatcatct cattggcccg gtagtagaac aaccagaata taatatgttc agaagagata
      121 aggtcgaatc agaattttca aaaatatacc ataccgtcgg attgggtacc accatctgga
      181 gtccgttggc atcaggtatc ctcaccggaa aatatctgga cgaatttccg caaaatacca
      241 gaatgagcct cgaaactgtg ggtcgtctta aagaaaaagc ccttacagaa gcaaaactta
      301 acatcatcag acagttggac atactcgcaa aagaaacagg tatttcactt gcccggttat
      361 caatagcttg gtgtctgaaa aatccgaatg tatcgactgt tatattaggc gctagcaaaa
      421 tagctcaact tgaggagacg cttactgcta ctgcagtatt gccattgcta accgaagaaa
      481 tcatgctacg catagacgaa attctcggca acaaacccaa atagtcaatc tactttcaga
      541 ccggaatctg gagttctctt acaggtacta cccaattgtg tttgtcaggt aatgttccta
      601 agcgtacacc tttcagcaga tcataagcca actggccgat ttttctttct tcaacgggcg
      661 gcagtatcat ttttgtgtct ttgtactgaa tctcagccac atgagctacc catgcagcag
      721 tgcccgttcc gaatgcttct ttgaatgagc cgttgtgata tgcttctaca agttcttcga
      781 tgcttaccgg acgcttttct acttcatatc cttgatcttt gagcaaatca atgattgact
      841 cacgagttat acctcgcaat attgctccat ctgtcgaagg agtcactatt ttgtcaccga
      901 taacgaagaa gatattcata gtacctactt cctgcacgta tttaaattct tgggcatcga
      961 gccaaagtac ctgatcaaat cctttttctt ttgctaattt ggcaggtaac agagatgctg
     1021 cgtaatttcc tgaagtcttt gcctctccta ctccacctct ggctgcacgt acgtatttct
     1081 cttcggcaag tagtttaacc ggtttgttat aataaggccc taccggacct gaaaaaatta
     1141 tcattctgta aacatcagat gcctgtacgc cgatatattc acctgtagca aacatatatg
     1201 ggcggatgta aagtgcactt ccttctgcag ttggtatcca ttctctatca atggcaagca
     1261 atgtgtgtag tgcatccaga aacatatctt caggaaactc aggcatacac attctaactg
     1321 cagatgcatt cagacgtctt gagtgcattt ctggcctgat gagtactggt ttgccgtcgt
     1381 gttgcttggc tgccttcatt ccttcaaaga tggattgtcc gtaatggagt gcgaggttgg
     1441 ctggatgaat catgaaatgg ccaaatggta caattcttgg atttatccat tcttttccgt
     1501 tgtagtccgc aacatacatg tggtctgaaa aaactctgcc aaatggtatg ttgttaaaat
     1561 caacagatga gagttttgat gttttggtta gttcaatcgc gattggtgtc ctcattttag
     1621 tgatttttaa caaaattaga ttatttttac aatatatttc tcttttttga taaataattt
     1681 ataaaaattt tttttgaaac ccatttttga tgcttaaaca aaataatatt tcgtatttgg
     1741 ccttgtttaa tgattttgat atttttacca ggaacaacca gtttgccaac caaatgcctg
     1801 accgacgatt ttttttcgtg tacaaaagcg aggacacgcc ctatatcgga tttttacaag
     1861 acattatcaa ggctaccaaa ataaatggcg atgattatag aatcattgaa ttatctgaaa
     1921 atgtattact acgattaggc gttttttttg aaaatgagtt atgtgaatca attattttct
     1981 ttggctttac gaatgaagaa cttggcttcc ctacaaattt tagaaaatac aaaccattcc
     2041 tactaagaga aaaatcattt atcattgctg attcattatc aatcattcaa ccggacaaaa
     2101 ctttgaaatc agctctatgg cagagcctga aagaattatt caacatcgta tgaaaaagat
     2161 tatttttgcc acaggcaata tgaataagtt gaatgaagtg aggcagatcc ttcatgaaca
     2221 gtatcaaatc ctcagtcttg aagagcttga ctttcatgaa gaacttgctg aggatggtga
     2281 tacattcgaa gataatgccc gacaaaaagc cctcttcatc accgaaaaat ttggattgcc
     2341 ttgttttgcc gaagatacag gtctggaggt agatgctctg aatggtgccc caggtgtata
     2401 ttctgccaga tatgccggca cacaacgttc gcatgctgac aatatcaatc tgttactcaa
     2461 aaacctcgaa ggtcatacaa accgaaatgc aagattcaag acgatcattg cactgacgtt
     2521 cgataagcaa atctattatt tcgaaggtac agtcaatggg agaatagcat accagccaag
     2581 cggcaatgca ggatttggat acgacccgat attcataccg gatggttacg accaaagttt
     2641 tggcgagctg cctacggaag tgaaaaacca aatttcgcac agggctattg ctgtagaaaa
     2701 acttttaaga ttcttacaag gaaaataggc tacacaaatg tatcttgact acttttctat
     2761 tcagctatga atacgttgtt atccttattg acatcagcca tccgtgctga agggtcaatc
     2821 tcaattttct tgatcttctt ggtcatatta aactgaaaag aatactcagt atccggccat
     2881 tgccaggctt tcaacacagt gtagggctca acttttgtat cattttcttt cgagccccgc
     2941 atcaatacca aaggtatgtt gtagcattca ctggtattgt cggtgtaggt aacaattaca
     3001 tcaagtggca tcggtacttt gcctatgcgt tggagtgtaa cggtatattt tccacgacgt
     3061 gttttcactt ctttaatccc atagtctatt gtatgcgttg aatttatcca gtactcatta
     3121 taccaatcga gtaccattcc ggatgatttt tctactattc ggagaaaatc attgggtttt
     3181 ggatgcttaa atgaccagtc gcggaaaaac tcaagcaaag ctttgtccag aaccttttgt
     3241 cctacaacgt atgacaactg atgaaggtaa acaaatcctt tgctatagga agccatacca
     3301 taagcggaat ttgtattata atggtctgca tgcgtgcaaa gtggttcttc tatcccttgt
     3361 ttgacaacgg agaaataaga attataggaa ggtgtaaaag gataattttt accttgaggg
     3421 tcgagtaatt ttgaagtaat aatgtcggaa gcataacttg taaagccttc atccatccat
     3481 gggtagagac tttcgtttat gccaagtaca ccctgatacc aagagtggag cagttcgtgt
     3541 gacactacac ccaccaggct tgccagagag cgtttacctg ttacgagtgt acccatcgga
     3601 tattccatgc cgccatcgcc tccttgtatg atataaaata ccggataagg atatttgcca
     3661 taaccggaac tagccaaatg gaacatttca ttaaggtaat gcggcaacct cttccatgat
     3721 tcggcatact caggataatc ttgatagtac agatatacaa caggtccgtt tgtaagtgtg
     3781 acggaggtac gtacataatc gcggtcagct gcccacatga aatcgtgtac acgttcagct
     3841 ctaaactgcc atagcttttt cttaccgtcg ggaagtaatt tgccatcaat atctgttgca
     3901 atacctgtac ctgctaccaa ataatcctta tcgatggtga tatttacatc aaaatcgccc
     3961 cagttaccat aaaattctct accaatataa gggttgggat gccagccgtc ttcatcatat
     4021 acacataact ttgggtacca ttgcgacata ctatagtcaa taccttcctt gttatcacgt
     4081 ccgcttctac gtatctgtaa aggcacctga ccattgaatg ccatttcaag aatgacggta
     4141 tcaccaggta aaataagttg accttcagta aatacctgaa gaatggtttc ctgggttgta
     4201 aagctaacgg gctttccgtt gagtgtcagc cgatctacgt tcagatttcc gtattctgat
     4261 tttttaagtt ttgatatcct gtcagctact cgtgggtctg ggtccggaat atttcttgaa
     4321 cgtacatcca tggcagaacc cggcttgaaa gcgttgaaat acaaatgata aaaaagctgg
     4381 ttgagtgtat cgggcgaatt attaaccaga atcagttttt gctgtccttt aaactggtgt
     4441 tttgatacgt caaaatcgat atccatcgtg taatggacat actgttgcca atatccatca
     4501 cgtgcctgta ccaaagcagt gcttaagatt aatattacca gaagaaaaaa ccgattaaaa
     4561 ctattattca tgttcagcat tttttcaggg tagaacccat ttgtaattgt atttgcaaat
     4621 cagcagaaag ataaatattc cgataaggaa taacgcaatt gaggcaaaaa cgttcatact
     4681 acttacttct atcaaaggct gagtatcgaa tgcggctttc ttataactta ctaatacagc
     4741 agcatagatg ttgttagatg catgcatacc gagtgcaagc tccattctgt tatcgaaata
     4801 tgagatcagc cctattccta gtccggcaag aaaataatag aacaacataa ttcctgcacc
     4861 atattcaacg atttcgggat tggaataatg aagaatggca aaaaaaatcg aactggaaca
     4921 cagcgcaacg aattttttat cgaaaaattt ataaaaatac tgcagaagat atgaccttac
     4981 gaatagttct tcaaagctgg tctgtgccgg tatcatcagg agggctacca acacgagcca
     5041 caaccagctt accggaagat catattggaa cgaataatcg tccggattaa gcatgaacat
     5101 gattatttcc agaaaaatct ggattgccag ccataaaaag aaaccgaaaa atatacgttt
     5161 aacagatatt tttaaaattc cgtcaggaat gaagagttta gaaaaatcag catgatgcaa
     5221 aaatctgaat accagataca aacctgccag gccggtacaa aaaggaagca acatcaaaaa
     5281 caaacgactt tggtcattgt cttcggtaat gaaactatcc ggataggctc cggctataaa
     5341 gcataatatt attataataa ctgaaaaaaa aagcctcaga taagaacttt tccaatgcaa
     5401 aatgttttta taataatgat tgacagctga taacatggag ttgtttgctt gatgaagaaa
     5461 cttcaaaact cagaattaat cacaaaaatc agttttttgt ttaataattg gattatattt
     5521 gtaaaaaatt gagtcgtatg aatgagaata atcagaaata tcttttaatt gtcggactaa
     5581 tatttaccat tgctttaagc agactagtac ctcatccatg gaattttacg cctgtaacgg
     5641 cactttttat attcgcaggt agttttgcaa ggaaaaaaat ggtaaatttt cttatcccat
     5701 tcagcgcggt cttattaagt gattttcttg tcaattattt tatatacaaa acagcgggct
     5761 tcacattttt ctatccgggt gcctggatgg tatatttggc ttatttggtc atattcctat
     5821 tcaaccatac catacacact gtaagctatc cgggtattgc ggctaaatca ctgatttcat
     5881 ccattatatt cttcctgatc actaattttg ctgcatggta tgcaagccct atgtatgcac
     5941 aaaattttgg tggccttatg acatcatata cagccggact tccgtttttc ggaaatggta
     6001 ttttgggtga tttattctac tctttcattc ttgtagcagc atataatatg ttgtatcgat
     6061 ctctgttaca aagggcagag ataagaagtc agcgttgaaa aaagagccta aaaagttcac
     6121 tacacgggag ggtttggaag taacagaaga tatagatttc tatgtagaaa atgggtatta
     6181 tgtcttcaca gcgcattatt tgctcaggcg aggttattgt tgccaaagcg gatgcagaca
     6241 ttgtccgtat ggtttcaaaa agaaaaaagc acacacagat tagcactcaa aatgtgcatt
     6301 gccgatttta caaaactccc ggatatcgtt aaattatgct tcaggatatg aaccatagca
     6361 gaccaggtta catgcccggt ttccaggata tttcttcagc attgaagtct ttggctattt
     6421 ttctactcaa tacaaacagg taatctgata gtctgttgag gtatttgata agctttgggt
     6481 ccacatcttc tatgtgatta agcgctacca tgcgtctttc tgccctacgg caaacagtac
     6541 gagcaatatg gcaaaaagaa acgaccgtat tgccacccgg caggataaaa ttggtaagtg
     6601 gcggcagatt ttcatccatt tcgtccatag ctttttcaag aacactaatg gcagattcac
     6661 gtaggtcggg tatgtaatcg gttgttttac cttcattagc gagtatgctt ccaatggtaa
     6721 acagattgga ttgtacagaa ttaatcaagt aacgctggtt ttcgtcctca aggttatcgt
     6781 acaagacgcc aagaaatgaa ttgagctcgt cgatggttcc gtacgcttca atacgtacat
     6841 gatctttcgg gactcttttt ccaccgaaga gagatgtttc gcccgaatcg cctgtttttg
     6901 tataaatttt cataaaaaga tatttaaata tttagccggc taagggtttt gcattgccca
     6961 caacacggat aatgttgcta ttttgttcat cagactccag ctgtccgtcg cgcagtctta
     7021 taatacggtg agcgtataaa gcaatatccg gttcgtgtgt tacgagcaca acagtattgc
     7081 cctttgagtg aatttcttca aacaatgcca taatttcaat agaagtcttg gtatcaaggt
     7141 tgccggttgg ttcatcagca agtataatgg ccgggtcatt gaccaaggca cgtgctacag
     7201 ccaccctttg gcgctgacca ccggagagtt catttggctt atga
//
LOCUS       DAOQQY010000132         7222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4201_length_7222_cov_6.779127, whole genome shotgun sequence.
ACCESSION   DAOQQY010000132 DAOQQY010000000
VERSION     DAOQQY010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4201_length_7222_cov_6.779127"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLE23_07410"
     CDS             <1..794
                     /locus_tag="PLE23_07410"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HMX83128.1"
                     /translation="RVIQLGLISRADMGFFGMLLTAVAALIGIYLVFASGPGLIPLIL
                     YGLIAGVYYTSVQGKFSFVNMAPGLAEFLIATTYGVFMTMGAFYVQTGYFTTQVFLIS
                     LPVAMLVTNVLLINQFQDAESDQKTQKNTLVVRVGKENAKNILISFFVMAYGIIAALP
                     LMGYAPYSLYFTFLSVPFAIQAIRYVSKNYNKTAVDLISGNAHTAIAHLFAGLLLVFA
                     FLLEGPQMVYPLVYLAASLLFVFWVWNYIESKRKAMDDFRQAIKG"
     gene            797..1777
                     /locus_tag="PLE23_07415"
     CDS             797..1777
                     /locus_tag="PLE23_07415"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HMX83129.1"
                     /translation="MLCIYHESTDPYFNVATDEYILKHIDEDCFMLWRNDNAIIVGAY
                     QNTLAEINYDYVKEHNISVVRRMSGGGAVYHDLGNLNFSFTKSGKDSNLSDFEKFTRP
                     IVNVIRDLGVDARFEGKNDLMIDGRKFSGNAAHIFKNKILHHGTLLFSSEMRNVAGAL
                     NVNPVKYQDKAIKSVPKRVTNISEHLPVKISLEEFTRKVMEYVVNNFPDTRMYQFTEE
                     DITAIQKLRDEKYATREWNFGKSPDYAFKKAIRTNGGLLEMNLDAKNGVIENVKIFGD
                     FFSEKGIEDVEHVLKGVLHEENELRKVLSGIDLDNHFRNISLDEIIGSMF"
     gene            1829..2380
                     /locus_tag="PLE23_07420"
     CDS             1829..2380
                     /locus_tag="PLE23_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HMX83130.1"
                     /translation="MKSTNKFSALHRLLHWIMAIAMTVLFITGFLRMKWMSKTSIKTA
                     LGDQFASTPLQDEQVRGVIKSIQGPMWEWHEIFANVMILSFLVRIVYMLVKGIRFPNP
                     FKSGISVRQRFQGMIYVYFYEFWRYLLLPVSVSRKNFLQLTKIRLRPCTNGDCTGCHC
                     LFYSISVVLHSQNTQAIRALHQK"
     gene            complement(2418..3530)
                     /locus_tag="PLE23_07425"
     CDS             complement(2418..3530)
                     /locus_tag="PLE23_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMX83131.1"
                     /translation="MKKTNKFLLSLSYTVLAFCLISIIHAQSPTRFNYQAVILDSAGT
                     TIKNKTIGVRISILTGNTTGPEVYSETHTPTTDAHGLISLEIGTGNVENGNLTAINWS
                     ANDYFIKTETDTEGGNNYSLTGISQLLSVPYALYAEKSGSNIQGPEGDAGKKGEDGDD
                     GQTAYQIWLSAGNTGTEADFLKSLTGPPGNPGSGNSFSHELGEEYGGGVIFHLWRDTD
                     GTEHGLIVSKEELYFNPQDPLEGIKWSNVINTEIGAPAQSSWDGVANTNAIKNQAGNN
                     SGAANLCLDYASGGFSDWYLPSITELQLVWTNRFHVNKTLDNITGAKRISTNATYWSS
                     TEYNTTKAYYIFFFNGLQDYSQNKGTGFKVRAVRRY"
     gene            complement(3600..4082)
                     /locus_tag="PLE23_07430"
     CDS             complement(3600..4082)
                     /locus_tag="PLE23_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83132.1"
                     /translation="MNNIYSKIISIIILLVSIGTSFSQSSINSGGGDLTGTGGTASFS
                     IGQVVYTSNTSTSGTIEQGVQHAYEIYTVSTDLTEAKLNAKVFPNPVNGNLNLQIDGY
                     KDKNLSYYLFDTNGKLIGTEPIEDTTTNIETKHLPQAVYLLELRSKTDRISCFKIIRQ
                     "
     gene            complement(4104..5192)
                     /locus_tag="PLE23_07435"
     CDS             complement(4104..5192)
                     /locus_tag="PLE23_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83133.1"
                     /translation="MRKTIYTILYNLLAVVVLAQAPNKFSYQAVITDASNQILKSKSV
                     GLRFSIIKDNASGPSLYSESHQTITSKYGQVSVEIGGGTVLSGAFGNINWKAGPYFLK
                     TEIDPEGGTNYTITGTIQLLSVPYALYSNLSGNSLPGPKGDTGEDGNKGQTGESAYDI
                     WLSLGNSGTKQDFINWMKGPKGQDGIGSGNDFIHFIGEAYGGGIIFHLWKDENETEHG
                     LIISKSDLHTNIEYSNFYKSTTEATSLWDGESNTNAILEEADYNPCAALLVVESKVEG
                     FNDWYIPAMDEMILMHRNLFTINKALSSISGGSPISVDKNSNLAEYWTSNSDESDTTL
                     GFSYRFVPGEFHKSPKIFTKKLRAIRKF"
     gene            complement(5224..6324)
                     /locus_tag="PLE23_07440"
     CDS             complement(5224..6324)
                     /locus_tag="PLE23_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83134.1"
                     /translation="MKKIVYLLFMIVITGSLNAQSPLKSGFQIVVRHGGIGGLVGNNA
                     VSVRISILKDSATATEAVYSEEHKVTTNSNGLASLTVGNGSVIKGNFSVIHWGQGGYF
                     IKTETDQTGTGTAYITTEIRPVTSVPYALYAEKSGSSTPGPKGNKGQKGQNGQNGQSA
                     YDLWINQGYAGTKGNYISSLKGPQGPPGSSGGYTHYIGEAYGGGVIFYLWKENNGTEH
                     GLICSLEDLSYTNVDSGQIWSNINNAAVDLSNEMMSSWDGNQNTEDIVNQPGHATSAA
                     KLCQDYIGGGLTDWYLPSIDELKILWQNRFNVNKALEIYSAFGLLDRTSGFYWSSTEF
                     GAGTAYSIGGWYGEIMPSDKSAPLFVRAVRSF"
     gene            6617..>7222
                     /locus_tag="PLE23_07445"
     CDS             6617..>7222
                     /locus_tag="PLE23_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX83135.1"
                     /translation="MKNLISLLTVVLTVFGINGTASVDGQSLFFERIYDETLAQASYI
                     IGDKQTGDAIVIDPKRDIDTYLAISKQNKLNIKQITETHIHADFLTGSRELAHVTGAK
                     LLLSDEGGQDWQYQFAHTSLRDGSIIHVGSIMLKVMHTPGHTPESISFLLMDGIKPVK
                     AITGDFIFVGDVGRPDLLEKAAGMVGTQEKGARDLFVSLNEF"
BASE COUNT         1997 a         1444 c         1469 g         2312 t
ORIGIN      
        1 cgagggttat acaactcgga cttatttcta gggcagatat gggatttttc ggtatgttgc
       61 tcacggcagt ggcagcactt attggtatct accttgtgtt tgccagcggg ccggggctta
      121 taccacttat attgtacgga ctgatagcag gtgtttatta tacttctgta caggggaaat
      181 tttcatttgt aaatatggct cccggactgg ctgagtttct cattgctact acttatggtg
      241 tatttatgac aatgggagcg ttctatgttc agaccggata ttttacaacg caggtatttt
      301 tgatatcatt gccggtagcc atgttggtaa ccaatgtctt actcatcaat cagtttcagg
      361 atgccgaatc tgatcagaaa acccagaaaa acacacttgt ggtacgagtt ggcaaagaga
      421 atgctaaaaa tattcttatt tccttctttg taatggctta tggcattatt gcagctctgc
      481 cgttgatggg ttatgcacca tattcgctat attttacatt cctttctgta ccatttgcta
      541 ttcaggcaat aagatatgtg agtaaaaatt ataataaaac agcagttgac ctcatttcgg
      601 ggaatgcgca tactgccatt gctcacttgt ttgcagggct gttacttgtt tttgctttcc
      661 tgctcgaagg cccacaaatg gtatatccgc ttgtttatct ggcagcttca ttgttgtttg
      721 tattctgggt ctggaattac atcgaaagca aaagaaaagc tatggatgat ttcagacagg
      781 caatcaaagg ataattatgt tgtgtatata ccacgagtca acggatccgt atttcaacgt
      841 agctactgac gaatatattc tgaagcatat tgatgaagat tgcttcatgc tgtggcgcaa
      901 tgacaatgcc attatcgttg gggcttacca aaatacactc gctgagatca attatgatta
      961 cgttaaagaa cataatatct cggtagtgcg ccgtatgtcg ggtggtggtg cagtgtatca
     1021 tgatttgggt aatctgaatt tttcctttac caaatcgggc aaagattcga atctgtctga
     1081 ctttgaaaaa tttacaagac ccatcgttaa tgttatccgc gatctcggtg tagatgcacg
     1141 cttcgaaggc aagaatgatt tgatgataga cggaaggaag ttttccggca atgcggctca
     1201 tattttcaaa aataaaattc tgcaccatgg caccttgctt ttttcttcag aaatgagaaa
     1261 tgtggctggc gcactcaatg tcaatccggt taaatatcag gataaggcca tcaaatcggt
     1321 acccaaacgt gtaaccaata tttctgagca ccttccggta aaaatttcac ttgaagaatt
     1381 taccagaaaa gtaatggagt atgtagtaaa taactttccc gacacacgta tgtatcagtt
     1441 tactgaagag gacataactg ccatacaaaa actccgtgat gaaaaatacg ccacccgcga
     1501 atggaatttt ggcaaatctc ctgattatgc gtttaaaaaa gccatccgta ccaatggcgg
     1561 tttgctcgaa atgaacctgg atgctaaaaa cggtgtgatc gaaaatgtta aaatttttgg
     1621 tgattttttc tccgaaaaag gaatagaaga tgtagagcat gtactgaaag gtgtattgca
     1681 tgaagaaaac gaacttagga aggtattgtc cgggatagac ctcgataacc atttccgaaa
     1741 tattagcctt gatgagatta tcggctcaat gttctgagga ctgtgtaaaa aacaactatt
     1801 taataccata aaaattattt ttctgttcat gaagtcaaca aataaatttt cagccctaca
     1861 caggttgttg cattggataa tggccatagc tatgacagtt ttgttcatca ctggttttct
     1921 aaggatgaaa tggatgagca agacaagcat caaaacagcg ctcggagacc aattcgcttc
     1981 tactccactc caggatgaac aggtgcgagg tgtcataaaa tctatccaag gacctatgtg
     2041 ggaatggcat gagatatttg ccaatgtcat gattctgtct tttcttgtac gtattgtcta
     2101 tatgctggtc aaaggtatta gattccctaa tccgttcaaa tcgggcatat ctgtaagaca
     2161 acgttttcag ggaatgatat acgtgtactt ttatgaattt tggcggtatc tgcttttacc
     2221 ggtatctgta tcgagaaaga attttttaca gcttacgaag atacgtttga gaccgtgcac
     2281 aaatggggac tgtactgggt gccactgttt attttactcc atttcggtgg tgttgcactc
     2341 gcagaacaca caagcgataa gggcattgca tcaaaaatga ttgggggcga ctaatatcat
     2401 cttattagca tttattgtta atatcttctc actgcccgta ccttgaagcc cgtacctttg
     2461 ttttgtgaat agtcttgaag accattgaag aaaaaaatgt aatatgcctt tgtggtattg
     2521 tattcggtac tgctccagta agtagcattg gtactgatac gtttggcacc ggtgatgttg
     2581 tccaaggttt tatttacatg aaatcggtta gtccatacca gttgcagttc ggtaatagat
     2641 ggcaaatacc agtcgctgaa tccaccggat gcgtaatcca ggcacaaatt tgctgcacca
     2701 ctattattgc cggcctggtt tttaatggcg tttgtgttag ccactccgtc ccacgatgac
     2761 tgggcaggag cgcctatttc ggtatttatt acattgctcc actttatacc ttcgagtggg
     2821 tcttgtggat taaagtatag ctcttctttt gacactatga gaccatgctc ggtaccatct
     2881 gtatcacgcc acaggtggaa tataactcca ccgccatatt cttctcctaa ttcgtgagag
     2941 aaactattgc ctgaaccagg gttgccggga ggcccagtca gtgattttag gaaatcagct
     3001 tcagtacctg tattgccagc actcagccag atctggtaag cagtctgtcc gtcatctccg
     3061 tcttcacctt tcttgccggc atcaccttcg ggaccttgta tgttgctgcc acttttttct
     3121 gcatataggg catagggcac gctgaggagt tgactgattc ctgtcaggct atagttgtta
     3181 cctccttcgg tgtctgtttc tgttttgatg aaataatcgt ttgccgacca gttaattgcc
     3241 gttaaatttc cattctccac attgccggta cctatctcta gtgatatcaa gccgtgtgca
     3301 tcggtggtgg gtgtatgtgt ttcggaatat acttccggcc cggttgtatt tcctgtaagt
     3361 atgctaattc tgacacctat tgttttgttc tttatagtag tacctgcact gtcaaggatg
     3421 acagcctggt aattaaacct ggttggagac tgggcatgaa tgatacttat taaacagaag
     3481 gcgagaacag tataactcag tgagagtaaa aatttgtttg ttttcttcat gacgctttag
     3541 ttgtcattat taaaaaatag atttttgagc aagcttttac aaagtactac ttttgacatt
     3601 tattgtctga tgatcttaaa acaggatatc ctatcagttt tgctgcgaag ttccagcaga
     3661 tatacagcct gtggcaaatg tttggtttcg atgttggtag ttgtatcttc tataggttct
     3721 gtaccaataa gcttaccgtt tgtatcgaac aggtaatagc tcaaattttt atccttatag
     3781 ccatctatct gtaagtttag atttccattc acagggttcg gaaatacttt agcattcagc
     3841 ttggcttctg tgagatcagt gcttacggtg tatatttcgt aggcatgctg tacgccttgc
     3901 tcaatggtac cacttgtaga agtatttgaa gtgtaaacta cctggccgat actaaatgaa
     3961 gcagtaccgc ctgtgcctgt caaatcacca ccaccgctgt taatggatga ttgtgaaaat
     4021 gaagtgccta tagacacaag taggattatg atgctgatta ttttagaata aatgttgttc
     4081 attactatct gtttatttta aaattaaaat tttctgatgg cgcgcagttt ttttgtaaaa
     4141 atctttggag acttatggaa ttcacccggg acaaaccggt atgaaaaacc aagagttgta
     4201 tcactttcat cactattgct cgtccaatat tctgcaagat tagaattttt atcaaccgat
     4261 atagggcttc cacctgaaat actgctcaat gctttattga tagtaaagag gttgcggtgc
     4321 atgagtatca tttcatccat ggccggaata taccaatcgt tgaagccttc tacctttgat
     4381 tcgacaacaa gcaatgcggc acatgggttg tagtctgcct cctccagaat ggcattggtg
     4441 tttgattcac catcccataa actggtagct tcagtagttg atttgtaaaa gttactgtat
     4501 tcgatgttgg tatgcaggtc acttttactt attatgaggc catgttctgt ttcattctca
     4561 tctttccaca aatgaaaaat gatacctcca ccgtatgctt caccaataaa atgtatgaaa
     4621 tcattgcctg agcctatacc gtcctggcct tttgggcctt tcatccaatt gataaaatct
     4681 tgcttagtgc cactattgcc aaggctgagc cagatgtcat aagcactttc acctgtttgt
     4741 cctttgttac cgtcttcccc agtgtcgccc ttagggcctg gtagcgaatt gccacttagg
     4801 tttgagtaca atgcgtacgg aacactgagt aactggatag taccggtgat ggtatagttg
     4861 gtgccacctt ccgggtcgat ttccgttttt agaaaataag gccctgcttt ccagttgata
     4921 ttgccaaagg cacctgacaa aaccgtacca ccgcctattt ccaccgatac ttgcccgtat
     4981 ttgctggtga ttgtttggtg gctttcgcta tacaggctcg gacccgatgc gttatctttg
     5041 atgatactaa agcggagacc aacacttttg cttttgagta tttgattgct tgcatctgtt
     5101 attacagctt gataactgaa tttgttaggt gcctgagcca aaacgactac tgcaagtaga
     5161 ttgtacaaaa ttgtatatat tgtttttctc atttcctttt gatttaaatt aatagtgtgc
     5221 caatcagaat gatctgactg ctcttacgaa aagcggagcc gacttatcgc tcggcataat
     5281 ttcaccatac cagccaccaa ttgaataagc agtacctgcc ccaaattctg tactcgacca
     5341 ataaaaccca cttgttctgt ccagtaaccc gaatgcacta tatatttcca gggccttatt
     5401 gacattaaat ctgttctgcc aaagtatctt cagttcatca attgaaggca gataccagtc
     5461 tgttaatccg ccaccgatat agtcttgaca cagttttgcg gcactggtgg catgccccgg
     5521 ctgattgaca atatcttctg tattttggtt tccatcccac gaagacatca tttcattcga
     5581 caggtcaaca gctgcattgt ttatattgct ccaaatctgt ccggagtcaa catttgtata
     5641 gcttagatct tcaagagaac agattaagcc gtgttctgta ccattatttt ctttccatag
     5701 atagaatatt acaccgccgc catacgcttc gcctatgtaa tgtgtatatc cacccgacga
     5761 accaggaggt ccttgaggtc cctttagcga gctaatataa tttccttttg tgcctgcata
     5821 tccctgattt atccaaagat cgtaagcact ttgaccattt tgaccgtttt gccctttttg
     5881 ccctttattt ccttttgggc cgggagtcga gctgcccgat ttttcagcat acaatgcata
     5941 aggtacactt gttacaggcc ttatttctgt tgtgatgtat gctgtacctg taccagtttg
     6001 gtctgtttct gttttaataa aatatcctcc ttgcccccag tgaataactg aaaaatttcc
     6061 tttgataacc gagccattac ctaccgtaag tgaagcaaga ccattgctat ttgtcgtcac
     6121 tttatgttcc tcgctataaa cagcttctgt agcagttgct gaatccttaa ggatgcttat
     6181 tcttactgat actgcattat ttcctaccaa gccaccaata ccaccatgcc ttactacaat
     6241 ctgaaaaccg gatttcagtg gtgactgagc attaaggctt ccggtaataa caatcataaa
     6301 taaaagatat actatttttt tcataaaaat gatcatttta tgtatgataa ataattatat
     6361 tataataaaa cctataactt tcattacaaa tttaaggaat aatattaata aatatataaa
     6421 aagtacatat tttaacatat tatcatatct gcatatatat tgaggaagat agttttttgg
     6481 aaaattttaa tctcaaaaat caaacaaaaa attaccctta tttgtaacgt tcaccgaact
     6541 gtaacttaag tttcataccg ggtaagataa tcacctgaac tttgtaccat taatcaaata
     6601 aaaaaattca taaaatatga aaaatttaat ctctttacta acggtagttt tgacggtatt
     6661 tggtattaac gggacggcgt cggttgatgg tcagagtttg ttcttcgaac gtatttatga
     6721 cgaaacactc gcgcaggcca gctatattat tggcgataaa cagacgggtg atgctatagt
     6781 cattgatcca aaacgcgaca tagataccta tttggcaatt tcaaagcaga acaaactgaa
     6841 tatcaaacag ataaccgaga cgcatattca tgcagatttc ctgacgggct caagagaact
     6901 tgcgcatgtc acaggagcca agttgctttt gtcggatgaa ggtgggcaag actggcaata
     6961 ccagtttgcg cacacttcac tcagagatgg aagtatcatc catgtaggca gtataatgct
     7021 taaagtcatg cacacgccgg gtcatacacc tgaaagtatt agctttctac ttatggatgg
     7081 cattaagccc gtgaaagcca ttacaggtga ctttatattc gtaggtgatg ttggtaggcc
     7141 ggatcttctc gaaaaagcgg cgggtatggt aggtacgcaa gaaaaaggag cacgtgacct
     7201 gtttgtttca ttaaatgaat tc
//
LOCUS       DAOQQY010000133         7152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4269_length_7152_cov_4.635902, whole genome shotgun sequence.
ACCESSION   DAOQQY010000133 DAOQQY010000000
VERSION     DAOQQY010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4269_length_7152_cov_4.635902"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PLE23_07450"
     CDS             <1..1053
                     /locus_tag="PLE23_07450"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMX83136.1"
                     /translation="NTGEGGLSPFHLKHGGDLIWQIGTGYFGCRHEDGTFNPDLFAEK
                     AKHPHVKMIELKISQGAKPGHGGILPASKNTEEIAAIRHVKPHTAVVSPPYHSAFNTP
                     VEMILFIQKLRELSGGKPVGFKICIGHKAEFISICKAMVALDLYPDFITVDGGEGGTG
                     AAPQEFSNYIGAPMLDGLDFVHNILTGLDIRKHIKIIAAGKITSAFHIARAMALGADT
                     CNSARAMMMAIGCIQALLCNTNRCPTGIATQDPTLTVGIDVADKKVRLANYHAGTVKN
                     FVEILGASGLDDMKNITRGHIYRRVSLNQMLTYEEIFPSIKVGSMLHNQIPEKYKWDF
                     AHSNKDKWGISYPESA"
     gene            complement(1466..1615)
                     /locus_tag="PLE23_07455"
     CDS             complement(1466..1615)
                     /locus_tag="PLE23_07455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83137.1"
                     /translation="MYNFLLVSAASPFDNNNPENPDADKETKFCPPAQAMLTSCRFLM
                     VNGKW"
     gene            1815..2228
                     /locus_tag="PLE23_07460"
     CDS             1815..2228
                     /locus_tag="PLE23_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMX83138.1"
                     /translation="MKNTIYLAAICLFAWISIQATDNTDYGQLQDYPKPANLKVLPKN
                     ISHEELEATMKAFNQALGVKCGFCHAPRKDGEKGLDFASDANPKKDVARSMMKMTNKI
                     NKKYFKDFKKDGVLLQIGCQTCHNGQAEPVMRKMH"
     gene            complement(2354..5131)
                     /gene="acnA"
                     /locus_tag="PLE23_07465"
     CDS             complement(2354..5131)
                     /gene="acnA"
                     /locus_tag="PLE23_07465"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HMX83139.1"
                     /translation="MSFSKAKSVREISVNGKNYKYSSLKDLNHDNVKHLPFSIRILLE
                     NVLRNFDAFSITEEHVETLLNWTPAPNDKDIPFMPARILMQDFTGVPAVVDMASLRAE
                     FVRQGKDGQKINPAIPVDLVIDHSVQVDYYGTDYAYDKNVTLEFDRNKERYELLKWAQ
                     KGLNNFTVVPPGMGICHQVNLEYLSKGVAQRDGWLFPDSLVGTDSHTPMVNGIGVMAW
                     GVGGIEAEAAMLGQPIFFTCPEVVGLKLTGKIPNECTATDLVLSIANLLRKTGVVGKF
                     VEVFGEGLEHLTVTDRATISNMSPEFGCTVAYFPIDDRTLEYMHATNRSEEQIRIVEA
                     YCKENMLWRTGNEDIRYSAVVELDLTTLAPTVSGPKRPQDKILVKDLADKFCEVLKSE
                     FSRDYTSHADRQESSWLKEGGSGTEFVFGKVPLKGESVQEVEVDSAHHAVRIKQPHRE
                     FLLSDGSIVIAAITSCTNTSNPAVMVGAGLLARKAVEKGLRTKSWVKTSLAPGSQVVT
                     KYLERSGLNVDLEALRFHTVGYGCTSCIGNSGPLPPSIATAVDKGELVVASVLSGNRN
                     FEARVHPQVKMNFLMSPMLVVAYALAGRVDIDLMNDPIDFDPNGQPVYLRDIWPSRDD
                     IQETINECMKQEDFQEVYDVIFDGSTDWQNLEVNLDQNFEWDQQSTYIKEAPFFENLK
                     ATPDDITDIDNARVLLYLGDSVTTDHISPAGSFKETSAAGAYLTEKGIPKEDFNSYGS
                     RRGNHEVMMRGTFANVRIKNKIADKEGGFSRYIPTGEVKTVFDTAMQYQQDGTQLIIL
                     AGKEYGSGSSRDWAAKGTFLLGVKAVIAESYERIHRSNLVGMGVAPLVFVDGQNAETL
                     GLDGTESYSIHGLANDLVPHKILDVKAVHASGKVTSFKVEARLDSAIEIEYYRNQGIL
                     QYVLRQYLKNN"
     gene            5545..5982
                     /locus_tag="PLE23_07470"
     CDS             5545..5982
                     /locus_tag="PLE23_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013407616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMX83140.1"
                     /translation="MKPRINISKVAPKAIEAMIGLEKYIHASGLDKTIYELIKTRASQ
                     INGCAYCINMHTRDAIKLGETTQRLFVLDAWRETQLYTEKERAALALTEAMTLIAGKE
                     VPDDIYDNAASLFTEQELAAIIMTIVTINAWNRISITSRTPLD"
     gene            6122..>7152
                     /locus_tag="PLE23_07475"
     CDS             6122..>7152
                     /locus_tag="PLE23_07475"
                     /inference="COORDINATES: protein motif:HMM:NF020677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PNGase F N-terminal domain-containing protein"
                     /protein_id="HMX83141.1"
                     /translation="MTRYFLLFFAFFLTTSLLAQLDLSKEPITKITYLVSYNGQTDTS
                     QNPIWVLASNNKVLVTSRDQMLGKSNYPREMMLVMPVQKIYSQYAFLNDSESVYMTDS
                     SSIGKQQFEFSEETKQILGHTCKKAKTVINSNTIELWYTTNAGFNAAPGLLGQSLGLV
                     LQQVRNGNTVITATDISKQNIQLPDYMTKAVSHKYNKLDYTDLLWRSRFTTIPLFDNQ
                     QINFSDTFTAREGILRFANGTIVLRKVKFPVLSATSQIFLDLSERSTGDAYDRTGTVF
                     LIAQDKKQSFLDGLQQGASVLPSYENGNGKKYQGVITTDSYSPAAELMRFFTPFGVGH
                     FNDRLTLKD"
BASE COUNT         2082 a         1752 c         1390 g         1928 t
ORIGIN      
        1 aataccggtg aaggcggcct gagcccgttt cacctcaagc atggtggtga ccttatctgg
       61 cagataggca ccggctactt cggctgccgg cacgaagacg gcactttcaa tcccgacctc
      121 tttgccgaaa aagccaaaca tccgcatgta aagatgatag agctaaaaat atcacaaggc
      181 gccaagccgg ggcacggtgg catcttgcct gcctcaaaga ataccgaaga gatagcagct
      241 atccgacatg tgaagccaca cacagctgta gtatctccac cttaccactc agccttcaac
      301 acacctgttg agatgattct gttcattcaa aaattaagag aactatcagg tggcaagccg
      361 gtaggtttca agatttgcat aggtcataaa gcagagttta tctctatttg caaggctatg
      421 gtagcacttg acctctaccc cgatttcatc accgttgacg gtggtgaagg tggcacagga
      481 gcagccccgc aggagttttc gaactatatt ggcgcaccta tgcttgacgg acttgatttc
      541 gtccacaaca tacttacagg gcttgacatc agaaaacaca taaaaattat agcagccggc
      601 aagataacgt cagcattcca tatagcaagg gcaatggcat taggtgcaga cacctgcaac
      661 tcggcacgtg ccatgatgat ggccataggc tgcattcagg cactgttgtg caataccaac
      721 cgctgcccta ccggaatcgc cacacaagac ccgacactca ccgtaggtat agacgttgcc
      781 gacaagaaag taagactggc caattatcat gcaggcactg tcaaaaactt tgtagagata
      841 cttggtgcct ctggcctaga tgatatgaag aatattacca gaggccatat ttaccggaga
      901 gtttcactca atcagatgct gacctacgaa gaaatttttc catctattaa ggtgggctct
      961 atgctacaca accagatacc tgaaaaatac aaatgggact ttgcacactc caataaagac
     1021 aagtggggca tctcgtatcc cgagtcagct taatacattt tacaaattta cactcacaag
     1081 aggattttca tgttgggaat cctctttttt gcgaatcaat ctgcagggtt gcaataaacg
     1141 aatcacttcc ttacaattaa gcattacaac cagaagagga atatgtatca gggcgtgccc
     1201 ctatcgggtc aggctatccg ccttacgttg tgccggcttc tatccttcac gcgcattcaa
     1261 ttaaaaatta aaaattaaaa attaaaagtg ggatattgtt acacagtatt attcagcatt
     1321 ttttgaacta ttaacaagtt aagtgtatga agcaaatact ttgccgaggc aagcagaaag
     1381 ttgtacaaaa ttctccgttt tgtttgcctc tttcctaaag ggagcaaaac gaagattttg
     1441 atgggaaatt cgcaccactt acccttcacc acttaccatt caccatcaaa aaccggcatg
     1501 aggtcagcat agcctgagcg ggtggacaga atttcgtttc cttgtcagca tctggatttt
     1561 caggattatt attatcaaaa ggtgatgccg ccgaaacaag cagaaagttg tacaaaaaga
     1621 acaaattaac tacttaccac ttaccactta ccacttacca ttcactactt accttttacc
     1681 aataaaaaaa gtaagaattt aatatcagct atcattcaag aaaaattttc catacagatg
     1741 acaattctgt gacaaaatac attatcttta tccagcgaat caattattta aacaattcta
     1801 atttctatac caacatgaaa aatacaattt atttagccgc aatttgcctt tttgcatgga
     1861 tcagcattca agcaacagac aatactgatt acggacaatt gcaggactac ccaaagccgg
     1921 ctaatcttaa agtgctgcca aaaaacatca gccacgaaga gctcgaagcg actatgaaag
     1981 ctttcaacca ggcactgggt gttaaatgcg gattctgtca cgcaccgcga aaagacggcg
     2041 aaaaaggcct ggattttgca agcgacgcta accctaaaaa ggatgttgca agatcaatga
     2101 tgaagatgac caacaaaatc aataaaaaat acttcaaaga ttttaaaaaa gatggagttt
     2161 tgctacagat tggctgccaa acctgtcata atgggcaagc cgaacctgtg atgcggaaaa
     2221 tgcactaatt tgggcgggca ttgttacctc aacccttata ccctaccaaa attataattt
     2281 ggattacaaa aaaaatgcgg attgagcctt attcgatctc aatccgcatg attattttta
     2341 ctagatccct ttatcagttg ttcttcaagt actgtctcaa cacatactga agtattccct
     2401 gatttctgta atattctatt tcaattgctg agtcgagtct tgcttctacc ttgaagctgg
     2461 ttaccttacc cgatgcatgc acagccttta cgtccagtat cttgtgcggc acaagatcat
     2521 ttgccaaacc atggatgctg taactttcgg ttccgtccag tccgagtgtt tcagcatttt
     2581 gtccgtcaac aaatactaat ggggcaacac ccatacctac aagattactt ctgtgtatcc
     2641 tttcataact ttctgcaata acagccttta ctccaagaag gaatgttccc tttgctgccc
     2701 agtcacgtga cgaacccgag ccgtattctt taccagcaag tatgataagc tgtgttccgt
     2761 cctgctgata ctgcatagct gtgtcaaaaa ctgtcttcac ttcaccggta gggatataac
     2821 ggctaaatcc gccttccttg tctgctatct tgtttttgat gcgcacgttg gcaaatgtgc
     2881 cacgcatcat tacttcgtgg ttgcctcgtc gtgaaccata cgaattgaaa tcctcttttg
     2941 gtattccttt ctcagtcaga tatgcgccgg cagcagaagt ctccttgaac gaccctgcag
     3001 gcgatatgtg gtctgtagtg actgagtcac caaggtacaa gagtactctg gcgttgtcaa
     3061 tatcagtaat atcatccggt gtggccttca gattttcgaa gaaaggtgct tctttgatat
     3121 acgttgactg ttgatcccat tcaaaattct gatcaagatt aacttcaaga ttctgccagt
     3181 ctgtagagcc gtcaaatatc acatcgtaca cttcctggaa gtcctcctgt ttcatacatt
     3241 cattgattgt ctcctgaata tcatcccttg atggccatat atcgcgcaga taaaccggtt
     3301 gcccattcgg gtcaaagtct atcgggtcgt tcataaggtc tatgtcaact ctgccggcaa
     3361 gtgcataagc gaccacaagc attggcgaca tcaggaagtt catcttcacc tgcggatgta
     3421 cacgtgcttc aaagtttctg ttacctgaca aaacggacgc taccacgagt tcacctttgt
     3481 caactgctgt agcaatggaa ggaggcaatg gacctgaatt gccaatacat gatgtacagc
     3541 cataaccaac agtgtgaaaa cgcaatgcct caaggtcaac attcagacct gagcgttcta
     3601 gatatttggt caccacctgc gaacccggag caagtgatgt tttaacccaa gatttggtcc
     3661 gcagtccctt ctctacagct tttctcgcca atagaccggc tccgaccatt acggcaggat
     3721 tcgaagtatt cgtacaactg gtgatggctg caataacgat gctaccatca cttagcaaaa
     3781 actctctgtg tggttgcttg attcttacag catgatgcgc agaatcaact tccacttctt
     3841 gtacggattc tcctttgaga ggcactttgc caaatacaaa ttcggtacct gaaccacctt
     3901 ccttcagcca tgatgattcc tggcgatctg cgtgagatgt ataatcgcgg ctgaactctg
     3961 actttaatac ttcacagaat ttatctgcca gatcttttac aaggattttg tcctgaggac
     4021 gcttagggcc agacaccgtt ggtgcaagcg tagtaaggtc gagctctact actgcagaat
     4081 agcggatatc ctcattaccg gttctccaaa gcatattttc cttacaatat gcttctacta
     4141 ttcttatttg ctcttcactt ctgttagtag cgtgcatgta ttcgagtgtt ctgtcatcga
     4201 ttgggaaata ggctaccgta caaccgaact caggcgacat gttggagatg gtcgcacggt
     4261 ctgttaccgt taggtgctca agtccttcgc cgaatacttc tacaaatttg ccgacaacac
     4321 cggtttttct aaggagattg gctattgaca acaccaagtc ggtggcagta cattcattag
     4381 ggattttacc tgttagcttt agccccacta cttccggaca agtaaagaat ataggctgac
     4441 caagcatagc ggcctcagct tcgataccgc ctacacccca ggccatcacg cctattccgt
     4501 ttaccatcgg tgtgtgggag tccgtaccta ccaaagagtc aggaaagagc caaccatctc
     4561 tctgagcaac acctttcgac aggtattcga ggttcacctg gtggcagata cccattcccg
     4621 gaggtacgac agtgaaatta ttaagacctt tctgagccca cttgagcaat tcgtatcttt
     4681 ccttatttct gtcaaactct agcgttacat tcttatcata agcataatcc gtaccataat
     4741 aatcaacttg taccgagtgg tcgataacga ggtcaaccgg tatggcagga ttgatcttct
     4801 gaccgtcttt accctggcgt acgaattcgg cacgcagtga tgccatatcc actacagccg
     4861 gtacaccggt gaagtcttgc ataaggatgc gggctggcat gaatggaatg tccttatcgt
     4921 tcggggcagg tgtccagtta agcaaggttt cgacgtgctc ttcagtaatg ctgaatgcgt
     4981 cgaaattgcg caatacgttt tcgaggagaa tccgtatgct gaacggaagg tgcttaacat
     5041 tgtcatggtt taggtctttc agtgaactgt acttgtagtt tttaccgttt actgaaatct
     5101 ccctgactga ttttgctttc gagaaactca tattttatta tttgaagatt atttacagac
     5161 ttctttgaat cattttccgg cagttttcat tgacttttac tgcaattctc tttaaaaacc
     5221 gcaaatataa cgatattttc ttaacaatgt ttaaagaaat aagatgactt gcatcatagt
     5281 ttgctgccgt ttatctgtat tggtcatgga tggagattgt tggtgtacaa gttctgtatt
     5341 caatcattca aagaccgaaa ccaccgtaca tttcccttaa attattgatg gcggctgtgt
     5401 ggatccaata tgtttgaatt ggcaatcaat atttaattac caacaatatt ttacacccaa
     5461 aaaacttatt caccgctcgt ttgtataaat actaacatct cataaaatta ttatttcaat
     5521 acaacccagt aaactaaatc agccatgaaa ccacgtataa acataagcaa agtcgcacca
     5581 aaagcaatag aagccatgat tgggctcgaa aaatacattc acgccagtgg ccttgacaaa
     5641 accatctacg aactcatcaa aacacgcgct tctcagatca acggttgtgc ctactgcatc
     5701 aatatgcaca cacgcgatgc catcaaactc ggcgaaacaa cccaacgact gttcgtgctc
     5761 gatgcctggc gcgaaaccca actctatacc gaaaaagaac gtgctgctct cgcactcacc
     5821 gaagccatga ccctgatagc cggtaaggaa gtaccggatg atatctacga taacgcagcc
     5881 tccctattca ccgaacaaga attggctgcc atcatcatga ccatcgtcac catcaatgcc
     5941 tggaaccgta tatccatcac ctcgcgtacg ccacttgatt gattcaagca aaaccagtca
     6001 tttccaacaa ggttcggata acataagaca acatttaaca aggtccgcaa gtaaggtttc
     6061 cgaagtttta ttgctaattt tgccatgata taaacaaaat accaccatac aaatccggaa
     6121 aatgacgcga tattttcttc tgttctttgc tttttttctc accacaagcc tcctcgccca
     6181 gcttgacctt agcaaagaac ccattaccaa aatcacctat ttggtcagct acaacggaca
     6241 aactgataca agccagaatc caatctgggt acttgcatcc aataacaagg tgctcgtcac
     6301 ttcacgcgac cagatgctgg gcaaaagcaa ctatccacgt gaaatgatgc tcgtcatgcc
     6361 ggtacaaaag atttattcgc agtatgcatt tctcaatgac tcagaatcag tctatatgac
     6421 agacagcagc tctatcggca aacaacaatt tgaattctca gaagaaacga aacagattct
     6481 cggacacact tgcaaaaaag ctaaaaccgt cataaactct aacaccatcg aactctggta
     6541 caccaccaat gccggattca atgctgcccc aggcttactc ggtcaatcat tgggtctcgt
     6601 actccaacaa gtgcgcaacg gcaataccgt catcaccgct acagacattt caaaacaaaa
     6661 tattcagcta ccggattata tgacgaaggc tgtttcacac aaatacaaca aactcgacta
     6721 taccgacctc ctatggcgaa gccgttttac gaccatccca ttgttcgaca accaacagat
     6781 caacttttcc gatacattca cagcccgaga aggcattctc cgctttgcaa acggcactat
     6841 tgtgcttcga aaagttaaat ttccggttct gagtgccacc tcacagatat ttctcgacct
     6901 gagcgaacgc tcgacaggtg atgcctacga ccgcaccgga acggttttcc tcattgcaca
     6961 ggacaaaaaa caatccttcc tcgacggact tcagcaaggc gcctccgttc ttccctccta
     7021 tgaaaatggc aatggaaaga aataccaggg cgtgattacc accgacagct acagccctgc
     7081 cgctgaactc atgcgcttct tcacaccttt cggtgtcggt catttcaatg accggctcac
     7141 gctcaaagat aa
//
LOCUS       DAOQQY010000134         7129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4292_length_7129_cov_6.533644, whole genome shotgun sequence.
ACCESSION   DAOQQY010000134 DAOQQY010000000
VERSION     DAOQQY010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4292_length_7129_cov_6.533644"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLE23_07480"
     CDS             <1..418
                     /locus_tag="PLE23_07480"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HMX83142.1"
                     /translation="IGTIFKKNATISAAMGGCQAEIGVSSSMSAAGLTECLGGTPDQV
                     LMAAEITMEHHLGMTCDPVGGLVQIPCIERNTMGAIKAITASNIALEGDPASARVSLD
                     DVIHTMWETAKDMNSKYKETSEGGLAVQIPVNLAAC"
     gene            553..1470
                     /gene="gldA"
                     /locus_tag="PLE23_07485"
     CDS             553..1470
                     /gene="gldA"
                     /locus_tag="PLE23_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HMX83143.1"
                     /translation="MSVVVKGLTKYYGDQAALRNISFEVEKGEVLGFLGPNGAGKTTT
                     MKILTCFINQSEGSALVNGYDVEKQPLMVKRSIGYLPEGNPLYKDMYIKEYLHFVGSV
                     FKVPKLSRRVDEMIEQTGLTPERNKLIGQLSKGYKQRVGLAQALIHDPQVLILDEPTS
                     GLDPNQLVEIRHLIKELGKQKTIIFSTHIMQEVQAICDRVVIINKGEIVADDSIETLR
                     EKVKGFKVLFIEFQENVEIGKLRMLTGVKKVESLGGNKFHIFSDSDKDARPVVSKFAT
                     DNGLTMLEIRTEMSSVEDVFQKLTQGKNG"
     gene            1473..2204
                     /gene="gldF"
                     /locus_tag="PLE23_07490"
     CDS             1473..2204
                     /gene="gldF"
                     /locus_tag="PLE23_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HMX83144.1"
                     /translation="MRSIYLKEINSFFSSLIAYLVIGGFLALLGLLLWIFPDDNILEA
                     KFASLDSLFNVAPLVLMFLIPAITMRSFAEENQTGTIELLVTKPVADWQIILAKFLAC
                     LTLVIIAILPTLVYYVSVYLLGSPVGNLDTGAIMGSYIGLVFLGACFVSIGIFASSLT
                     KNQIVAFIIATFLCFLFHWGFSYTSRIPVFVGRFDDLIQSLGIDFHYGSISRGLVDSR
                     DVVYFFSITGFFLFLTLLMVERRKW"
     gene            2219..3946
                     /gene="gldG"
                     /locus_tag="PLE23_07495"
     CDS             2219..3946
                     /gene="gldG"
                     /locus_tag="PLE23_07495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HMX83145.1"
                     /translation="MKLLSDKKGNKSSGSHRLTQAVMLALVLILLNIISSYFFFRIDL
                     TGDKRYTLTEPTKNLLKGVKDIISVKVLLDGEFPAGFKRLQQSAKEMLDDFRSVNPNI
                     TYDFQDPNAGTPEQINDMRAKFKELGIIPTNLRIQQDNKTEEKIIFPYAIVNYGTRKY
                     IVNLLESNVAGTSPEVVLNNSVGLLEYKFANAIQKLLKREKQNILFTAGHGELQQLET
                     TDLESTLRESYNTGRVNLDTIVQIKNAIDLLIVARPRTEFDEKTKFKIDQYVMNGGKV
                     LWLIDKLAVSLDSLRRPGNAYVPYDYSLNLDDMLFKYGVRIQNNLILDMECSKIPLQT
                     GMIGNTPQYDQFPWYYFPAVMPKSNNPIVKSLDRVNLYFPSTIDTIQTKTPVKKEILL
                     ASSKYSRLQPNTSRLNFEVLRYDPEPERFNKPYQPMAVLLEGKFPSLFENRVSPEMEA
                     GLKELGMKFEAVSKETRMIVVSDGDIARNSFNPATGEMRPLGFNQYERRVYANKNFLI
                     NCVDYLIEGRGIIEARAREVKLRLLDTVKVRDEKGFWQLINVLLPVVLLVLAGMSYFW
                     FRKKRYASN"
     gene            3975..4982
                     /locus_tag="PLE23_07500"
     CDS             3975..4982
                     /locus_tag="PLE23_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HMX83146.1"
                     /translation="MKRLVILILLLLIGSFAVYKLTREDPYSTLGKDNDFAVQDTAQI
                     GKIFIGDRNGNSALLTRNGVWWMVNQKYKVNPPVMESLLKTIYGLETLYRPPVEAAKT
                     MVKNLATKSVKVEIYNKSNKLIRVFYVGGVTPDESGTYMIMEGEDQPFVVGQKGKDGG
                     IKVSFFTEEEKWRDKGIFRYAVDDLKIVSVEYPKLKNKSFILTKSQSGYDVVPLIEGV
                     PKINAKPNPSKVESYLTGFKKQIAEAFENKLPRRDSLMQLLPFSILRTEDMKGQRHEV
                     KLYPIIPTNRDGQALLYQGKPVPHDRYYAVVDNHDIMLVQELVFGKLLWSYDYFFDKP
                     K"
     gene            4979..6244
                     /locus_tag="PLE23_07505"
     CDS             4979..6244
                     /locus_tag="PLE23_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMX83147.1"
                     /translation="MMKRSILSILILFLSFQYLSAASDPPAWGFFGHRLINRMAVFTL
                     PPEMISFYKANIEYLTEHAVDPDKRRYAARLEAARHYMDMDQFGYYPFESLPRTWYPF
                     LATYANVYVVLDDKDTVRVMGEGVWRGSNKEYKVISDSLLALLDTQYYRVPLRDMHQF
                     AAFSINKAYRHDEWEYSRDSLKGIFPNAAFLSRVTKVFGNASFSEHGLLPYNLDEMFN
                     RLVKAFVAMDKNAILRLSAELGHYIGDAHVPLHTTKNYNGQLTNQDGIHAFWESRIPE
                     LFADEEFDFLVGKAQFIKETNDYFWKVIFDSNEGVKLVLDIENNLKKSFPEDQQFCYE
                     NRLQQVIRTQCRDFAKAYMVALDGQVEDRMREAIISVGNIWYTAWVTAGQPDLKLLKD
                     AQLSAQEQADQEELEKKFQQGKGYGRNHE"
     gene            6344..7120
                     /gene="aroE"
                     /locus_tag="PLE23_07510"
     CDS             6344..7120
                     /gene="aroE"
                     /locus_tag="PLE23_07510"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMX83148.1"
                     /translation="MEITESVSKNDHTLYGLIGYPLGHSFSAMFFKEKFARLGLINHD
                     YRLFELQDISAIRNFTVQYKSLRGFNVTIPHKQSILDYLDVISPDAMNIGAVNCVKIK
                     DGIWTGYNTDALGFQIALSMFLQENCITNPKQAIILGNGGSSKAVQWVLNQENIPFVV
                     ASRRNTSDTIHYDEITPEFFEKIGIIINTTPLGMYPATGTAPELPYHALNNKHLLIDL
                     VYNPEKTLFLTRGSAMGCKIMNGKKMLEEQAEASWNIWNT"
BASE COUNT         2215 a         1329 c         1531 g         2054 t
ORIGIN      
        1 aataggcacg atttttaaga aaaatgcgac tatttctgca gcaatgggtg gttgccaggc
       61 tgagattggt gtttcgtctt cgatgtctgc tgccggactt accgaatgtc tgggcggcac
      121 acccgatcag gtacttatgg cagctgaaat aaccatggaa catcaccttg gaatgacctg
      181 cgatcctgtt ggtggcctgg tacagatacc ctgcattgag cgtaatacaa tgggtgccat
      241 caaagccata acagcttcga acatagcttt agaaggagat ccggcatcgg caagggtaag
      301 tttggatgat gttatacaca ccatgtggga aacggcaaaa gacatgaatt cgaagtataa
      361 ggaaacatca gaaggtgggc ttgcagtaca gattccggtc aatctggcag cttgctagga
      421 aaaaaattgg cgttttttta tacttaagac gactcttctg aattctggtg aaaacaattg
      481 tgcaggtttt attagttttg cgattcttaa agtcggtcgg ccgatcattc agccaaccgg
      541 aaataaactt atatgtcagt agtagtaaaa ggtttaacta aatattatgg cgatcaggct
      601 gcactccgca atatctcttt tgaggtagaa aagggtgaag tacttggctt tcttggtcct
      661 aatggtgccg ggaaaaccac taccatgaag atattaactt gttttattaa tcagtcagaa
      721 gggtcggcac ttgtcaatgg atatgatgtt gaaaaacaac cgttgatggt taaacgcagt
      781 attggttatc tacctgaagg caatccgctg tataaagata tgtacattaa ggagtatctg
      841 cattttgtag ggagtgtgtt caaggtgccc aagctgagca gaagggtgga tgaaatgatc
      901 gaacaaacag gccttacacc cgaacgaaac aaacttatag gacagttgtc aaaaggctac
      961 aagcaaaggg tagggcttgc acaggcattg attcatgatc ctcaggtatt gatactggat
     1021 gagccaactt caggccttga ccctaaccaa ttggtcgaaa tccgtcacct gattaaagaa
     1081 ctcggcaaac aaaagaccat catattctct acgcacatca tgcaggaagt acaagcgatt
     1141 tgtgaccgtg ttgttatcat caataaaggt gaaatcgtgg cagatgattc aattgaaacc
     1201 ttgcgtgaga aagtaaaagg ttttaaagtg ctgtttattg aatttcagga gaatgttgag
     1261 atcgggaagc tcagaatgct taccggagtg aagaaagtag aatcgttggg agggaataaa
     1321 tttcacatat tcagtgattc tgataaagat gcccgacctg tggtatcaaa atttgctacc
     1381 gacaacggac taacaatgct tgaaatccgt accgagatgt caagtgtgga agatgtattt
     1441 caaaaactaa cccaagggaa aaacggatag taatgcgtag tatttatttg aaggaaatca
     1501 acagtttttt tagttcgctt atagcttatc tggttattgg tgggttcctt gctttgttag
     1561 gccttcttct ttggattttt cctgatgaca acattctaga agctaaattt gcatcgcttg
     1621 attctttgtt taacgtggca ccgttggttc tgatgtttct tattccggct attactatgc
     1681 gttcgtttgc tgaagaaaat caaaccggta cgatagagtt actggtaaca aagccggttg
     1741 ccgactggca gataatcctg gccaaatttc ttgcatgcct gactttggta attattgcca
     1801 ttttacctac tttggtgtac tatgtgtcgg tttacttgct tggatcacct gtaggtaacc
     1861 ttgatacagg agcaatcatg ggttcgtaca ttggtttggt attcctggga gcctgttttg
     1921 tcagcattgg catattcgct tcttctctta ccaaaaatca gatcgtagcc tttattatag
     1981 ctactttttt atgctttttg tttcattggg gatttagtta tacgagcagg atacccgttt
     2041 tcgtagggcg atttgacgat cttattcagt ctcttggcat cgattttcac tacggctcta
     2101 tcagccgtgg ccttgttgat tcgcgagatg tggtttactt tttctctatt accggatttt
     2161 ttctgttttt gacattattg atggtagagc gaagaaaatg gtgatttaaa acatgagtat
     2221 gaaattatta tcagataaga aaggaaataa aagttcggga tcacatcgac ttacacaagc
     2281 tgtgatgctg gcactggtat tgattcttct caatattatt tcttcttatt tcttcttcag
     2341 aattgacctg accggagata aaagatatac gcttaccgaa ccaaccaaaa atttgctcaa
     2401 aggtgtcaaa gacattattt cggtcaaagt attgctcgat ggagaattcc ctgcgggttt
     2461 caaaagactc caacaatcgg caaaagaaat gttggatgat tttcgttcgg tcaacccaaa
     2521 tatcacgtat gactttcagg acccaaatgc cggtacacct gaacagatta atgacatgag
     2581 agcaaaattc aaggagcttg gcatcatacc tactaatctg agaattcagc aagataataa
     2641 aacggaagaa aaaataatct tcccttatgc aattgttaac tatggtacca ggaaatatat
     2701 tgtcaactta cttgaatcaa atgtggcagg cacatcaccc gaagtagttc ttaataattc
     2761 tgttggcctt ttggaatata aatttgcaaa cgctatccaa aaattgttga agcgtgaaaa
     2821 acaaaacatc ctttttaccg ccggacacgg agaactccag caactagaaa ctactgacct
     2881 tgaatcgacg ctaagggaat cctacaacac aggaagggtt aaccttgata ccattgtaca
     2941 aattaagaat gccattgacc ttcttattgt agcaagaccg cgaaccgaat ttgatgaaaa
     3001 aacaaagttc aagattgatc agtatgtaat gaatggcggt aaagttctgt ggttaatcga
     3061 taaacttgcc gtatcacttg acagccttcg tcgtcccggc aatgcctatg taccttacga
     3121 ttattcgctt aacctcgacg acatgttatt caaatatggt gtgaggatac agaataatct
     3181 gatcctggac atggaatgct ctaaaatccc attgcagact ggaatgatag gcaatacacc
     3241 tcaatacgac cagtttccat ggtattattt tccggcagtg atgccaaaat ccaataatcc
     3301 tatcgttaaa tctcttgatc gggtaaatct gtatttcccg tccaccattg ataccataca
     3361 gactaaaacc cctgtcaaaa aggaaatact gttggcatca tcaaaatatt caagacttca
     3421 acccaatact tccaggttaa actttgaggt acttaggtac gatcctgaac ccgaacgatt
     3481 taacaaacca tatcagccga tggctgtttt actggaaggt aaatttccat cattgtttga
     3541 aaaccgggtt tcacctgaaa tggaagccgg cctcaaagaa ctaggtatga aattcgaagc
     3601 agtgagcaaa gaaaccagaa tgatagttgt gtctgatggc gacatagccc gtaattcatt
     3661 caacccggca acaggtgaaa tgcggcctct aggattcaac caatatgaaa gaagagtata
     3721 tgccaataaa aattttctca taaactgtgt tgactatttg atagaaggca gaggaattat
     3781 agaagcccgt gcaagagaag taaaattacg tttgctcgat acagtcaagg ttagagatga
     3841 gaagggcttc tggcagttga taaatgtgtt gctgccggtc gtgttacttg tattggccgg
     3901 gatgagctat ttttggttcc ggaaaaaaag gtatgcgtct aactaatgca taaaagttaa
     3961 ttaatcaaga agaaatgaaa cgtttagtta ttctcatatt gttattgctt attggttcat
     4021 tcgccgttta taaacttact cgcgaagatc catattcaac attgggcaag gataatgact
     4081 tcgctgtaca ggataccgct caaataggca aaatttttat tggcgaccga aacggtaatt
     4141 ctgcactact aaccagaaat ggtgtttggt ggatggtcaa tcaaaaatat aaagtaaatc
     4201 ctcctgtgat ggaatcattg ctaaaaacca tctatggtct ggaaacattg tatcgccctc
     4261 ctgttgaagc tgccaaaaca atggtgaaaa acctggcaac caagtcggta aaagtagaaa
     4321 tatataacaa aagcaacaaa ctcatccggg tattttatgt cggtggtgtt acgccggacg
     4381 aaagcggcac ctatatgatc atggaaggtg aagatcaacc atttgtagta ggtcaaaagg
     4441 gtaaggatgg tggaataaaa gttagtttct ttactgaaga agaaaagtgg cgtgacaaag
     4501 gtattttccg ttatgcggtt gatgacttga aaatagtaag tgtagagtat cctaagttga
     4561 agaacaaatc gttcatactt accaagtcac aatcagggta tgatgttgta cctctcattg
     4621 aaggcgtacc aaagataaat gccaaaccaa atccatcaaa agttgaaagt tatcttacgg
     4681 gttttaaaaa gcaaatagct gaggcatttg aaaataaatt gccacgacgc gattcactca
     4741 tgcaactgct tcctttcagc atcctcagga cagaggatat gaaaggacaa agacatgaag
     4801 tgaaattata tccgatcatc cctacaaatc gggatggaca ggccttgctc tatcagggaa
     4861 aaccggtacc tcatgacagg tattacgcag tagtggataa tcacgatatc atgttggtgc
     4921 aggagttggt gtttggcaaa ttgttgtggt cttacgatta tttttttgat aagccaaaat
     4981 gatgaagcgc agtatccttt caatccttat acttttcctg tcatttcagt atttatccgc
     5041 tgcatctgat ccgcctgcat gggggttctt tggtcacaga ctcatcaacc gaatggcggt
     5101 atttacattg ccacccgaaa tgatatcatt ttataaggcg aatatcgaat atctgacaga
     5161 acatgccgtt gacccggaca aaagaaggta tgctgccaga cttgaagcag ctcgacatta
     5221 tatggatatg gatcagtttg ggtactaccc atttgaaagc ctgcccagaa cttggtatcc
     5281 ctttttggct acttatgcca atgtgtatgt tgtccttgat gacaaggata ctgtcagagt
     5341 gatgggtgaa ggtgtgtggc gtggttcgaa taaggaatac aaagtgataa gtgactcttt
     5401 gctcgctttg cttgacacac agtattatag agtaccatta cgtgacatgc atcagtttgc
     5461 cgcttttagt attaataaag cctaccgaca tgatgaatgg gaatatagcc gtgatagtct
     5521 aaaaggaata ttcccgaatg cagcattcct cagtagagtg acgaaagtct ttggtaatgc
     5581 aagtttttca gaacatggtt tactacctta taatttggat gaaatgttca acagattagt
     5641 taaagctttt gtggcaatgg acaaaaatgc cattttgagg ctatctgcag agcttggcca
     5701 ttatatcggt gatgcccacg ttccattgca cactacaaaa aattacaacg gccaattgac
     5761 caatcaggac gggattcatg cattttggga aagccggatt ccggaacttt ttgcggatga
     5821 agaatttgat tttttagtcg gaaaggccca gtttattaaa gaaacaaacg actatttctg
     5881 gaaggtcatt ttcgacagca atgaaggagt gaaactcgtg ttggacatag aaaataacct
     5941 caaaaaatct ttccctgagg accagcaatt ttgctacgaa aacagattgc aacaagtgat
     6001 ccgcacacaa tgccgtgatt ttgcaaaggc ttacatggta gcactcgatg gtcaagtgga
     6061 ggacagaatg cgagaggcta taatctcagt cggtaatatc tggtacacgg cctgggttac
     6121 agccggacaa cctgatctta aactgctcaa agatgctcaa ctctcagctc aagaacaagc
     6181 cgaccaggaa gagcttgaga aaaaattcca acaaggtaaa ggatatggaa gaaatcatga
     6241 gtgaacgtat aacataagta tagttttgag tttattttgg attgtaattt tttcacattt
     6301 taatctcttc aaggagatct taactacatt atcctgaaag aaattggaaa taactgaatc
     6361 cgtatctaaa aatgaccaca ctctgtacgg tctgattggt tatccgcttg gccattcttt
     6421 ttccgctatg ttttttaagg aaaaatttgc aaggctcggg ctcattaacc atgactaccg
     6481 tttatttgag ctacaagata tttcggcaat aagaaatttt acagttcaat acaaatctct
     6541 gagaggattt aatgtaacca ttccacataa acagtctatt cttgattatc tggatgtcat
     6601 cagccctgat gcaatgaata tcggagcagt caattgtgtc aaaatcaaag atggaatctg
     6661 gacaggttat aacacggatg cccttggatt tcagattgct ttaagtatgt ttttgcaaga
     6721 gaattgtata acaaatccaa agcaagcaat aatattaggt aatggcggaa gttcaaaggc
     6781 tgtgcaatgg gtgctcaacc aggaaaatat accctttgtt gttgcctcca gacggaatac
     6841 atctgacacc atacattacg atgagattac acccgaattt tttgaaaaaa ttggaatcat
     6901 catcaataca acaccacttg gcatgtatcc ggccaccgga actgctcctg aattgccata
     6961 tcatgcactc aacaacaaac atttattgat tgatcttgtt tataatcccg agaaaacact
     7021 atttttgact cgcggatctg caatgggttg taaaataatg aatggtaaga aaatgcttga
     7081 agagcaggcc gaagcatcat ggaatatttg gaatacctaa cataaatca
//
LOCUS       DAOQQY010000135         7119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4310_length_7119_cov_6.857446, whole genome shotgun sequence.
ACCESSION   DAOQQY010000135 DAOQQY010000000
VERSION     DAOQQY010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4310_length_7119_cov_6.857446"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..963
                     /locus_tag="PLE23_07515"
     CDS             121..963
                     /locus_tag="PLE23_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83149.1"
                     /translation="MKHKFIYTLFSLAILAHIWMGHAIGAGKVLKKNLTGSPLNTETC
                     GKCHNKKGAFDIKTTLTLLDANNKQVTKYKGDQTYTLNIVVTGNGLKGGPVAPYYGFQ
                     AVALNNQNNTAGTFTVPSIVQMIDIDGRSVIEHKTPKEAGTWSVTWKAPAKNSGPVKF
                     YMCGVACDGNKAVTNDTTATTVLTLQESVATSNEILHDFSVKKLFVSNNVLQAELDIA
                     RSDEYRFEIIDINGKLLYSTSEKFATGEISKELDVSSISAGVYILSVSNRVESTARKF
                     FKNN"
     gene            complement(1020..1487)
                     /locus_tag="PLE23_07520"
     CDS             complement(1020..1487)
                     /locus_tag="PLE23_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83150.1"
                     /translation="MRFITEKEIDEAIEAFENDEAKYDLALSSFATTQPGVTAYLLGE
                     DFDSLSDDERELMLYMCIIMYNAIIHEPSELPSPTTDDVEEAEDANWKKLENITAKKF
                     SDRLDVFFEETSQEDLLAFFEDVLIEEEEMITKEGREPIFIALKSILDTWQAE"
     gene            complement(1514..3127)
                     /gene="pckA"
                     /locus_tag="PLE23_07525"
     CDS             complement(1514..3127)
                     /gene="pckA"
                     /locus_tag="PLE23_07525"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HMX83151.1"
                     /translation="MKDQGIKNPSFQLSDIGINNYGTAYWNLTPEELIEHTIKKDMGQ
                     LSDSGAIIIKTGKFTGRCPKDKFTIKDNTTVNEVAWGNVNIPMEEADFDKLYQLVTGY
                     FENKEIYVRDNYACADEDYRVNIKVVTEYPWSNQFANNMFLRPATDDLADFKHDWLIL
                     CAPGCLADPAVHNTRADNFSAINFTKKIILIGGSGYTGEIKKGIFTVLNFELPVYHNV
                     LPMHCSANIGDNGNTAVFFGLSGTGKTTLSADPNRKLIGDDEHGWSDKGIFNFEGGCY
                     AKCIDLSAEKEPDIYRAIKHGAILENIIFKNGTREPDYADSSITENTRVSYPIYHINN
                     IAEPSVGGHPSNIFFLTCDAFGVLPPLSKLTPGQAMYHFISGYTAKVAGTEVGITEPT
                     KTFSACFGAPFLPLHPTRYAEMLGKKMKNHNVNIWLVNTGWTGGEYGVGNRISLKYTR
                     AMITAVLSGKLDNAEYKTQDIFGLNYPASCPDVPENILNPINTWENKENYYAKANMLA
                     KSFIENFEKFSEMASDEILAAAPKVISFA"
     gene            complement(3235..4407)
                     /locus_tag="PLE23_07530"
     CDS             complement(3235..4407)
                     /locus_tag="PLE23_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMX83152.1"
                     /translation="MDIYEKKSRWKVYLALAGIAIIAISMVYTIYISRQLAEGEKSKV
                     ELYKKVLESLNDPENLNKDISFETELINSNILKNIPVIFVDESGVIYGGANWGSARDT
                     NMVFLQKKLEDIKSSGRQPIQGEGYARYIYYEHTRLLKLLTIFPIIQVFLILVFVFIG
                     YLAFSSAKKSEQNQVWIGMAKETAHQLGTPISAILAWIEHLDQLTSEKSESAEIIGEL
                     KNDVNRLELIADRFSKIGSTPTLKKINLIEELEKNRTYMQKRAPRKVLFDFPEINQTP
                     LYAEINPHLFDWVIENLIRNALDAMDGSGKIQCSISQNENSVNIDLTDSGKGIPSSKW
                     KTVFQPGYTTKKRGWGLGLSLAKRIIESYHHGKIFVKHSAPDQGTTFSIQLPYLKN"
     gene            complement(4407..5396)
                     /locus_tag="PLE23_07535"
     CDS             complement(4407..5396)
                     /locus_tag="PLE23_07535"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HMX83153.1"
                     /translation="MSFISNRICAIGVQSIPFVENYDIVDYEKSENWAALPTKQDSAD
                     LTPKDLATHEMPFDIDVFFLYPTSFTKADERWNATIDDAKINEKTDNASIKFQASIFN
                     KTGKVYAPRYRQAHIKAYFSKDHESARQALDFAYQDVRAAFIYYLEHYNNGRPFILAS
                     HSQGTTHAQRLIKEFIDKKPLQKQLIAAYLVGLPVLKNSFYTIKPCLTPEETGCFCSW
                     RTYKTGYEPKTNIPFDEIAVINPITWTTGLAPADKSLHKGSVLADFNQLKPKSQTAQI
                     YKGVILTNKPKFRGSFLFTSKNYHVGDYNIFYRDVQENSRLRAKTYLQTQQGN"
     gene            complement(5720..6907)
                     /locus_tag="PLE23_07540"
     CDS             complement(5720..6907)
                     /locus_tag="PLE23_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HMX83154.1"
                     /translation="MDYFKESLKLHKKNQGKIEVTSKIDVQTTEDLSLVYSPGVAEPC
                     IKISQNQEAVWDYTLKSSTVAIVSDGSAVLGLGNIGARAAIPVMEGKAMLFKKFADIN
                     GFPICLDTQDTEEIIRTVRLIAPVFGGINLEDINAPKCFEIENRLQDLGIPVFHDDQH
                     GTAIVVIAGLINACKVVKKKLEDLTVVVNGAGAAGVAIAKMLKCIGFSEDECTPVKEL
                     IMCDSTGIIHRDRKDLNKSKLELLTFSNSANRSGSVKDAIVGADVFIGVSVANLLTAR
                     DIKKMNKDAIIFALANPVPEIMPEEAYKGGAAVVGTGRSDLPNQVNNVLGFPGVFRGA
                     LDAKAKVISPSMKLAAAYAIAKCVKNPSRECVIPPCLDLNVAKEVAKAVKKAALKDVD
                     TYW"
BASE COUNT         1977 a         1337 c         1452 g         2353 t
ORIGIN      
        1 cattgtatct ttatattttg tgtaaatata cgaatatatt taagaacatt gccaatatta
       61 aaacaacaga tctcatcatt ccgtaataat aggcattaaa ctaacaataa accgaagaat
      121 atgaaacaca agtttattta tacactattt tcccttgcca tcctggctca catctggatg
      181 ggccatgcca ttggtgcggg caaggttttg aaaaagaatc ttacaggttc gccactcaat
      241 accgaaacat gtggaaaatg ccacaataag aaaggtgctt tcgacataaa gacgacactg
      301 actttgttag atgccaataa caaacaggtg acaaagtata agggtgatca aacatatact
      361 ttaaacatcg tagtaacagg caatgggctt aaaggtggcc ctgttgcgcc gtattatggt
      421 tttcaggcag tggcactcaa taatcagaat aatactgcgg gcaccttcac tgtacccagt
      481 atcgttcaga tgatagacat agacggacgt tctgttatcg aacacaagac tcctaaagag
      541 gctggtacat ggagcgtaac ttggaaagca cctgccaaga attcgggccc ggtaaagttt
      601 tatatgtgtg gtgtagcgtg tgatggtaat aaggctgtga ccaatgacac tactgctact
      661 acagtactta cacttcaaga atcagtagct acttccaatg agatattgca tgatttttca
      721 gtcaaaaagc tgtttgtaag caacaatgtg cttcaagccg agctagatat tgcacgaagt
      781 gatgaatatc gttttgaaat tatcgacatc aatggcaaat tgctttacag cacttcggaa
      841 aaatttgcta ccggcgaaat tagcaaagaa ctcgatgtga gtagtatctc agcaggcgtt
      901 tacatacttt ctgtaagtaa tcgggtagaa tctactgcca ggaaattttt caaaaataat
      961 taaagggagt gtagcgtagt aaagaggcag atacgtattt atatctgcct cttcgctcat
     1021 tattcagcct gccaggtatc gagaatgctt ttaagggcaa taaaaatagg ttcgcgtcct
     1081 tctttagtta tcatttcttc ttcttcgatc aatacatctt caaagaaggc aagcagatct
     1141 tcttgagatg tttcttcaaa aaatacatcg agtctgtcac tgaatttttt ggcagtaatg
     1201 ttttcaagtt ttttccagtt ggcatcttct gcttcttcca catcatcggt tgtcgggctt
     1261 ggcaattcgg atggttcgtg tatgatggca ttgtacataa taatgcacat atacaacatc
     1321 agttcccttt catcatcgga gagagagtca aagtcttcgc cgagcaaata tgctgttacg
     1381 cctggttgag ttgtagcaaa tgaggataat gccagatcgt attttgcctc atcattttcg
     1441 aatgcttcaa tagcttcgtc tatttctttt tctgttataa acctcatttt ctggatttgg
     1501 tgataataat caattaagca aaactgataa cttttggtgc tgcggccaat atttcgtcgg
     1561 aagccatttc actgaatttt tcgaaattct cgataaacga ctttgcaagc atatttgctt
     1621 tggcataata attttcttta ttttcccagg tgttgatagg gtttaggata ttttcaggta
     1681 catccgggca tgatgccggg taattgagtc cgaagatatc ttgggttttg tattcagcat
     1741 tatccagttt accactgagt acagctgtga tcattgctct tgtatatttg agtgaaattc
     1801 ggttgcctac accatattcg ccaccggtcc aacctgtatt gacaagccag atgttgacat
     1861 tgtggttttt cattttttta ccgagcattt cggcatatcg tgtagggtga agtggtaaaa
     1921 acggagcacc gaaacaggca gagaatgtct tggttggttc ggttatacct acctctgtac
     1981 cggctacttt tgcagtataa cctgaaatga aatgatacat tgcttgtcct ggtgtcagtt
     2041 ttgatagagg aggtaatacg ccgaatgcat cacaagtgag gaaaaaaata ttggacggat
     2101 gcccgccaac agaaggctca gctatattat tgatatggta gatagggtat gatactctag
     2161 tattttcggt gatagaagag tcggcataat caggttctct tgttccattt ttaaaaataa
     2221 tgttttctag tattgcacca tgctttattg ctctgtagat gtcaggttct ttttctgccg
     2281 ataagtcgat acatttggca taacatccgc cttcgaagtt gaagatgcct ttatctgacc
     2341 agccgtgttc gtcgtcacct atcaattttc ggttagggtc ggcagaaagt gtggttttgc
     2401 cggtacctga cagaccgaaa aaaactgcag tgttgccatt gtcgccaata ttggcactac
     2461 aatgcatggg tagtacatta tgataaaccg gaagctcgaa gttaagtact gtaaaaattc
     2521 ctttcttgat ttcgcctgta tagcctgacc caccaataag gattattttt tttgtaaaat
     2581 tgatagctga aaaattatca gctcttgtat tgtgtacagc aggatcggcc aagcaacctg
     2641 gagcacaaag aataagccag tcgtgtttga agtcggcaag atcatcggtg gctggcctca
     2701 ggaacatgtt gtttgcaaac tggttagacc acggatattc ggttacaact tttatattta
     2761 ctctgtagtc ttcatctgca catgcatagt tgtctcgcac gtatatttct ttattttcaa
     2821 aatatccggt aacgagttgg tatagtttgt caaagtcagc ttcttccatc ggtatgttaa
     2881 cattgcccca tgccacttca ttgacagttg tattatcttt aattgtaaat ttgtctttcg
     2941 ggcatctacc ggtaaatttt ccggttttga taatgatggc accagaatca gatagctgcc
     3001 ccatatcttt tttgatggtg tgttcgataa gttcttcggg tgttagattc cagtaggcag
     3061 tgccatagtt gttaattccg atatcagata attggaatga tggattttta attccctgat
     3121 ctttcataaa atgatttttt gcttgctaca caatgatttt tgaatattat ttcgtgtgtt
     3181 ataagaactg caaagttagt aaattattgt cgttttttgg ctgctgatgg cacgctaatt
     3241 ttttaaataa ggcaactgga tggaaaacgt tgtaccctga tccggagcag aatgctttac
     3301 gaatatttta ccatgatggt aagattctat aatgcgtttg gcaagagata atccaagccc
     3361 ccagcctctt tttttagtag tataacccgg ttggaacacc gttttccatt ttgaagacgg
     3421 aattcctttg ccagaatctg taaggtcaat attcacagaa ttttcattct gactgattga
     3481 acattgaatt tttccgctgc cgtccatagc atccagtgca ttgcggatca gattttcgat
     3541 aacccagtca aacaggtgtg ggttgatttc ggcatataac ggtgtttgat tgatttccgg
     3601 aaagtcgaac aacacttttc ggggtgctct tttttgcata taggtcctgt ttttctctag
     3661 ctcttcgatc aggttaattt ttttaagagt aggtgtagag ccaatcttcg aaaacctgtc
     3721 agcgatgagt tccagccggt taacatcatt tttgagttca cctataattt cagcgctttc
     3781 cgatttttcg cttgtcagtt ggtcaaggtg ttctatccag gcgagaatgg ccgatatggg
     3841 agtaccaagc tgatgagcag tttcttttgc catccctatc catacctgat tttgttcaga
     3901 ttttttggca gagctaaatg caagataccc aataaaaacg aatacaagga taaggaaaac
     3961 ttgaataatg gggaatattg tgaggagctt aagtaagcgg gtatgttcat aataaatata
     4021 gcgtgcataa ccttcgcctt gtattggttg tctgcccgat gatttgatgt cctctaattt
     4081 tttttgcaag aaaaccatat tggtatctct tgcactaccc caattggctc ctccgtatat
     4141 gacgcccgat tcgtcaacga atattacagg aatgttcttc agtatattcg agttgataag
     4201 ttcggtttcg aaacttatat ctttgttgag gttttcagga tcattgagtg actcgagcac
     4261 tttcttgtac aattctactt tgcttttttc gccttctgcc aattgtctgg atatatatat
     4321 ggtatatacc atagaaatgg caatgatggc tataccggct aaggcgagat aaactttcca
     4381 tcgggatttt ttttcgtaaa tatccatcaa ttgccttgtt gtgtttgtaa ataggttttt
     4441 gctcttaatc ttgaattttc ctgtacgtca cggtagaaaa tattataatc gcccacatga
     4501 taatttttag aagtaaatag gaatgaacct ctaaatttcg gtttattggt aagaataacc
     4561 cctttatata tttgggcagt ctgtgatttt ggctttaatt gattaaaatc agccagaacg
     4621 gatcctttat gcagagattt atcggcaggt gccaatccgg tggtccaggt gattgggttt
     4681 atgacggcaa tttcatcaaa cgggatatta gtttttggct catagcctgt tttgtaagtt
     4741 cgccagctgc aaaaacatcc ggtttcttct ggtgtgagac atggttttat ggtatagaat
     4801 gagtttttca ataccggtag tcctaccaga taggcagcta tcaattgttt ttgtaatggt
     4861 tttttgtcga tgaattcctt gatcagtcgc tgagcatgtg tagtgccctg gctatgtgat
     4921 gccaatatga aaggcctgcc attgttgtag tgttccagat aatagatgaa tgcagccctt
     4981 acatcctgat aagcaaaatc tagtgcttgt ctggcggatt catggtcttt gctaaagtag
     5041 gctttaatat gtgcctgtct gtacctgggt gcatacactt ttcctgtttt gttaaatatg
     5101 ctggcctgaa atttaataga agcattatct gttttttcgt taatctttgc atcgtcaatg
     5161 gttgcattcc acctttcatc tgctttagta aatgaagttg gatacaggaa aaaaacatct
     5221 atatcaaatg gcatttcatg cgtagccagg tctttgggcg tcaggtcagc agaatcttgc
     5281 tttgtcggaa gggcagccca gttttcagac ttttcataat ctactatatc gtaattttct
     5341 acaaatggta tagactgaac acctatggcg catattctat ttgaaataaa tgacattgcc
     5401 aaaatgagac tagtggatag aaaaattgag ttaaatgaca tcatatcaaa acaggaatgg
     5461 acagtttatt tctatataga aacgaaataa ttgctgtggt agttgtatga aggtaaaaaa
     5521 ttttaacctc aaggcaaatc actaggtgtg cgcacacatt tgttgaaact gaaatatgat
     5581 gtaacactct tacggttgta ggagtgataa acattgcggc agcaaaacat taacaaaaca
     5641 ccattttata gtttggatgt tttatccgat acgctcagaa taagttgaaa aattctgaat
     5701 tattttcagg cctaacacat taccaatatg tatctacatc ctttagggct gcttttttta
     5761 ctgccttagc cacttctttt gctacattga ggtcgagaca aggcggtatt acacattcgc
     5821 gagaaggatt ttttacacat ttggcgatag cataagcagc agccaatttc atggatggtg
     5881 aaatgacctt agcttttgcg tcaagtgcac ctctgaatac acccgggaaa ccaagtacat
     5941 tattgacctg attaggaaga tcgctgcggc ctgtaccaac tactgctgca ccacctttat
     6001 atgcttcttc gggcattatt tccggtacag ggttagcaag agcaaatatt atagcgtctt
     6061 tgttcatttt cttaatgtcg cgtgcggtga gcagatttgc aacactcacc ccgataaaaa
     6121 catctgcacc tactattgca tctttaacag aacctgagcg gtttgctgaa ttggaaaatg
     6181 taagaagttc tagttttgat ttattcaaat cttttctgtc gcggtgaatg ataccagtag
     6241 agtcacacat aataagttct ttgacaggcg tacattcatc ttctgaaaaa cctatacatt
     6301 taagcatttt ggcaatagct acaccggcag caccggcacc gttgactact accgtcagat
     6361 cttcgagttt tttcttgact actttgcagg cattgataag acctgcgata actactatag
     6421 cagtaccatg ttgatcgtca tgaaacaccg gtatgcccag atcctgtagt ctgttctcta
     6481 tttcgaagca ttttggtgca ttgatgtctt caagattgat acctccaaaa accggagcta
     6541 tcagtctcac tgtcctgatt atttcttcgg tatcctgcgt gtcgaggcag atggggaagc
     6601 cattgatgtc tgcaaatttt ttgaacaaca ttgctttacc ttccattaca ggaatggctg
     6661 ctctggctcc gatattacca aggccaagta ctgcgctccc gtcagataca atggctactg
     6721 tactcgattt gagggtatag tcccacacgg cttcctggtt ctgtgatatt ttgatgcaag
     6781 gttcagcaac acccggcgaa taaaccagag aaagatcttc ggtagtctgt acatcaattt
     6841 ttgatgtcac ttctattttt ccctgatttt ttttgtggag tttcagagat tctttaaaat
     6901 aatccattat tagttgattg gttagctatt actcaaaagt gcaaatccat catcaaaatt
     6961 acaaaaaata catgatagca gcagaaatct ttaaggttta atacgttgtt tgtatgttta
     7021 aagtttgaag taatccatta tttataaata tctgtcttaa aatctttttt ttcggttact
     7081 taaccgcggt acatgccttc agagttgaat ataagtgca
//
LOCUS       DAOQQY010000136         7112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4314_length_7112_cov_5.837891, whole genome shotgun sequence.
ACCESSION   DAOQQY010000136 DAOQQY010000000
VERSION     DAOQQY010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7112
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4314_length_7112_cov_5.837891"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PLE23_07545"
     CDS             complement(<1..1216)
                     /locus_tag="PLE23_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83155.1"
                     /translation="MTEVKYYTNDISLANKVMLFILFTVLLPYTAIAQLDIVDGWTQF
                     VASDDTRIIYVSADSGDDATAQYYDKYSQQIGSDPFHPSGNIKAFQTIQAAVLHLRNG
                     YPDWILFKKAEVFTNQSFGSTTKFGKNASEPMLIGSYGTSDIRPRILTGNQGFIDLAG
                     TSASYLAIVGLEIEPHSRTGTDEPIGIRIIEAPFTEILIEDCYISKYFTNLTVHDPLF
                     PSATTRKRLIVRRCVIADAYVSNASHANAMFIANVDSILFEENLLDHNGWNDTTPGAQ
                     PTGFRHNSYFQVGCTNLVFRENIVARAAATGGGFRCGGTIYNNTFLANPKNIQFGTHE
                     STIDWPSQSVTGDIGWNVVLDSRITGFETGKGITVQKAKNVHIHHNIISDYTPTGDYN
                     LALFLTESENLTA"
     gene            1432..2334
                     /locus_tag="PLE23_07550"
     CDS             1432..2334
                     /locus_tag="PLE23_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83156.1"
                     /translation="MKKNYLFLVVLGLLAASNLFSQSLWEDNFESYPLGNFLDGNGWV
                     REDGQDDWAQIVELDSIHGKSLQLFAPDTNLLGMFVTHNNNWANRTQGNNVFETNFDF
                     YTGNAQSEGLGQVVIANSNFYEIVSIGMLSGGNSLFIYADGLDTSLVENALPDTWYNI
                     TITYDKLSGEVIAQVDGGPKISGSGAADLDPEIFDFTALLASNVGIDNIKVSATKSSI
                     LNLPKVHQSGSTIAYPNPVHDVLNIRTDKNVAHITIYDIAGRVAGEFSTQTELDFSSF
                     TSGIFAVNILFEDGTQEVHKVIKK"
     gene            complement(2465..3505)
                     /gene="lpxD"
                     /locus_tag="PLE23_07555"
     CDS             complement(2465..3505)
                     /gene="lpxD"
                     /locus_tag="PLE23_07555"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMX83157.1"
                     /translation="MQISAKELSALLNGTLEGNPDVMVTGPSKIEEGIPGTLTFLANP
                     KYEAYVYTTKASVLLCGTDFVPSSPIAATIIRVENVYGSIALLLEKFASEVSSPTGIS
                     SQAFVDVTAVVGEGSSVGHFSVIEKNAVIGKNCTIHAQVFVGENVIIGDNVTLHSGVK
                     IYHNCRLGNNITLHSNVVIGADGFGFAPQEDGTYKKVAQIGNVVIEDDVEIGSNTTVD
                     RATMGSTYIRKGVKLDNLIMVAHNVEIGENTVIAAQTGIAGSTKIGRNCMIGGQVGIV
                     GHILIADRTKIQAQSGIARTIKEPDTAWYGSPAIDYADFLKAQVVFKKLPELQKRIQE
                     LEKALKAQEADD"
     gene            3609..4469
                     /locus_tag="PLE23_07560"
     CDS             3609..4469
                     /locus_tag="PLE23_07560"
                     /inference="COORDINATES: protein motif:HMM:NF013538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83158.1"
                     /translation="MEKRPTLRIGTEGTAFCKHKFNVINELLADHGYGAIPVMIQVTD
                     GRNDFIRNAEIALLNNEIDVAVHSLGKLSVNTTDGIRIGAVCAPHNPASCLLIRKEAY
                     LSGEDLFLKPGALVGVTPLSILQLTSLKSDLKVKIAEGTVIERINALQKAEFDAILVS
                     AFELDNCGADVSEFEILHLNPREFVPAAGQGVLALQVRANDEAILQAVRRVHDNKMFD
                     TISVERKIHKQLADVSKICLGVHCAKDHQNYYHVWAVMQSDANGIKKIQVSSSTSFEL
                     AENVVHQLLS"
     gene            complement(4494..4874)
                     /locus_tag="PLE23_07565"
     CDS             complement(4494..4874)
                     /locus_tag="PLE23_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMX83159.1"
                     /translation="MRKNKIIFWMATISIVVLEIILPFIAAGSTEIIRKVEHLGYPAA
                     FGFILMSFKIIGGFVLLYPRVPNIIKEFVYAGFAIDFIAAFVSVWAVDGIVQETVYPL
                     IALGVLSISYMYYQKLSENREVTV"
     gene            complement(5064..6260)
                     /locus_tag="PLE23_07570"
     CDS             complement(5064..6260)
                     /locus_tag="PLE23_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HMX83160.1"
                     /translation="MISVEEAKQLLLENVGILSAITVEVADASGYALEENIIAPTDSP
                     PFSQSAMDGFAVCLPEPFDATEAVKFMIIGETKAGDSPTEALLPGTAVRIFTGAPIPE
                     NTYCVVMQEKTTTINNKVEIALSALHKASNIRLKACEITKGTRAANKGMPINPAMCGF
                     LSTLGITHTQVARKPFISVLTTGNELKKPGAVLAPGQIYESNSFALQSAIKQCGYHTL
                     TIDTVADEESELMDKARKMLNESDVLILSGGISVGKYDLVAGVLQNLGVKKIFYKIAQ
                     KPGKPLYFGKQGEKLVFALPGNPAAALVCFYEYVLPALHKMSGHTRIGLRSIRLPLSK
                     DFLVKGDRALFLKAVVEGEMVGITEGQESNILMSFAVADALVYIPAGERIISKGGMVE
                     VHLLPT"
     gene            complement(6294..>7112)
                     /locus_tag="PLE23_07575"
     CDS             complement(6294..>7112)
                     /locus_tag="PLE23_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMX83161.1"
                     /translation="IGRSAAVFLAEHGFCVYGTVRKQEDADEIKALNNPNLIPLLMDV
                     TDKVQINTIKDQLKDIPLAGLVNNAGIAVSGPVKYVSEDKLRRLFDVNVFGLINVTQA
                     FIPNLELNGKINGTPGRIVNISSVSGLIVTPFMSPYAASKFAVEAFSDGMRRELNHTG
                     IKVCIIEPGPIKTKIWDKAMTEDTSDFKNEYSKMAQHRSGFIQNTIDNALPDVYTSRS
                     IYDALTDSNPKVRKIVTKNATMFKLIRKLPARWLDYFAGKRINMNQIEKNLKNH"
BASE COUNT         1975 a         1443 c         1489 g         2205 t
ORIGIN      
        1 ttgcagtcag gttttctgat tctgtaagga ataatgccag attatagtct cctgtaggcg
       61 tataatcaga gataatgttg tggtgaatat gcacgttttt tgctttttga acggtgatac
      121 ccttgcctgt ttcaaaaccg gtaatgcggc tgtcaagcac aacattccag cctatatcgc
      181 cggtgactga ttgagacggc caatcgatgg tgctctcatg tgtaccaaac tggatgtttt
      241 taggattggc caggaaggtg ttgttataaa tagtgcctcc acacctgaat cctccacctg
      301 tagcagctgc acgggctact atattctctc tgaacacaag attggtacaa ccaacctgaa
      361 agtatgaatt gtgtctgaaa ccagtaggct gtgctccggg tgttgtatcg ttccaaccat
      421 tatggtcgag cagattctcc tcgaacaaaa tactgtcaac attggctatg aacatagcat
      481 tggcgtgcga agcattgctt acatacgcat ctgcaatgac acatcgcctt acgattagcc
      541 tctttctcgt agtagcagat ggaaacaaag ggtcgtgtac tgtgaggttg gtaaaatatt
      601 tgctgatata acagtcttcg attaatattt ctgtaaaagg agcctcgatg attctgatgc
      661 cgattggttc gtcagtacct gttctgctgt gtggttctat ctccagacct actatggcca
      721 ggtaggatgc agaagtacct gcaagatcaa tgaatccctg gttgccggtc aggatgcgtg
      781 gccttatatc tgatgttcca tagctgccta tcaacatagg ttcacttgcg tttttaccaa
      841 attttgttgt agaaccgaaa gactggtttg taaatacttc agctttcttg aacaatatcc
      901 agtccgggta cccgttgcga agatgtaata ccgcagcctg aattgtttga aaagctttga
      961 tattcccgct gggatgaaaa ggatcagatc cgatctgctg agaatattta tcataatatt
     1021 gggctgtggc atcatcacct gagtcagcac ttacatagat tattcgcgtg tcatccgaag
     1081 caacaaactg ggtccatccg tcaacaatat ccaattgtgc aattgctgta tagggcaata
     1141 agactgtgaa taaaatgaac aacattactt tattagcaag ggaaatatca tttgtataat
     1201 attttacttc agtcataaat ttgtttgttt tagtatggat tgttatagtg aatgtcagta
     1261 tatcggaatt gcacaggtaa agatataaaa aattatattg tgaaacattt ttcaataatg
     1321 gaggtagcca gggtaaaatt tgaaggttaa ttttcaaaaa ttgtttggtc agttggaaaa
     1381 ttcaatttac atttgagaaa ataatattta aaaaaaatta attaatagaa catgaagaaa
     1441 aattaccttt ttttagttgt gcttggattg cttgcagcaa gtaacttgtt ttctcaatct
     1501 ttatgggaag ataactttga gtcttatccg cttggaaatt ttctggatgg taatggctgg
     1561 gttcgggaag atggacagga tgactgggca cagatagtag agttggattc tatacatggt
     1621 aaaagtctgc agttgtttgc cccggacacc aacttgctgg gcatgttcgt aacgcacaac
     1681 aacaattggg ctaaccggac tcaggggaat aatgtattcg aaacaaactt tgatttttat
     1741 acaggtaatg cacaaagtga aggtttgggt caggtagtga ttgccaactc taacttctac
     1801 gaaatcgtct ctataggcat gctttcaggt ggcaacagtc ttttcattta tgctgatggg
     1861 cttgatacaa gcttggtaga aaatgccttg cccgacactt ggtacaatat caccattact
     1921 tatgataaat tgtcaggcga agtcatcgct caggtggatg gtggcccgaa aatttcagga
     1981 agtggtgcgg ccgacctaga tccagagatt tttgacttta ctgccttact tgcctcaaat
     2041 gtcggaatag acaacattaa agtaagtgca accaaatcat ccatacttaa cttgcctaag
     2101 gttcatcaat ctggcagtac aattgcttat cctaatcctg tgcatgatgt tctaaacatc
     2161 aggacagaca agaatgtggc gcatattact atttacgata ttgcaggaag agtagcaggt
     2221 gaattttcta ctcagactga gttggatttc agtagcttca ccagtggtat atttgcagta
     2281 aatattcttt tcgaagacgg tacgcaggaa gtacacaagg tcatcaaaaa ataaatttct
     2341 gttgtttaca taatgaaatt ttaacactac atcaactacc cgctaagata cagccgtaag
     2401 tggtagtgta caccatacca taaaaagagt gcgatttcat ttgaattcgt actcttttta
     2461 tttattaatc atctgcttcc tgggctttaa gtgccttttc cagttcctgt attcgttttt
     2521 ggagttcggg tagttttttg aatacaacct gagctttaag aaaatcggcg taatctatag
     2581 ccggactgcc ataccaggcc gtatcgggtt ctttgatggt acgtgctatt ccgctctggg
     2641 cctgtatttt tgtacggtcg gctatcagaa tatgcccgac gatgcctacc tgtccgccga
     2701 tcatgcagtt gcgtcctatt tttgtgctgc ctgctatacc tgtctgggct gctatgacgg
     2761 tgttttcacc gatttcaaca ttatgtgcta ccatgatgag attatcgagt tttacgccct
     2821 tgcgaatgta agtagagccc atggtggcac ggtcaacggt tgtgttactg cctatttcaa
     2881 catcatcttc aatcacaaca ttgcctatct gtgctacttt tttgtacgta ccgtcttcct
     2941 gaggtgcaaa cccgaatccg tcagcaccaa ttacaacatt gctgtgtagt gtaatgttgt
     3001 tgcctaaccg gcaattgtgg taaatcttta cgccggaatg caaggtcaca ttatcaccga
     3061 ttattacatt ttcacctaca aaaacctggg catgtatcgt gcagtttttg ccgattacag
     3121 catttttttc tattacgcta aaatgcccca ccgaactacc ttcgccgaca actgcagtga
     3181 catcgacaaa agcttgtgat gaaatgccgg taggtgaact cacttctgat gcaaattttt
     3241 caagaagcag agcgatgcta ccatatacat tctctacacg gatgatggta gctgctatcg
     3301 ggcttgacgg gacaaaatca gtaccgcata gcaaaacaga cgcttttgta gtataaacat
     3361 aagcttcgta tttcggattc gccaaaaatg ttaacgttcc gggtatgcct tcttctatct
     3421 tacttggccc ggtaaccatt acgtcggggt tgccttccag tgtgccgttt aacagggcac
     3481 ttagctcttt tgctgaaatc tgcatgtgaa aaatttgaac aaaagtaaga ctaatttgtc
     3541 caaaattatt atccattttg gaaaaaggct aattttgcag ccaaattatt gtaactaaat
     3601 caagtatttt ggaaaaaaga ccaaccctac gcatcggtac agaaggaact gcattttgta
     3661 aacacaaatt taatgtaatc aacgaactat tagctgacca tgggtacggg gcaattccgg
     3721 tcatgattca ggtaacagat ggcagaaatg attttatccg aaatgccgaa attgctctgc
     3781 tcaacaacga gattgatgtc gctgtacatt ctttggggaa gctttctgtc aatactactg
     3841 acggtatccg gataggcgct gtgtgtgctc ctcataaccc tgcttcatgt ctgctcatcc
     3901 gtaaagaggc gtatttgtcg ggtgaagacc tgtttctaaa accaggcgcc ttggttgggg
     3961 ttacaccctt gagtattctc caactaacct ccctaaaatc tgatttaaag gtaaaaatag
     4021 cagaaggcac tgttattgaa cggattaacg cactacagaa ggctgaattt gatgccatcc
     4081 ttgtttcggc attcgaactc gataattgtg gcgcggatgt gagtgaattt gaaatcctgc
     4141 acctcaatcc aagggagttt gtgcctgccg ccggacaagg tgtactggca ttacaagtgc
     4201 gggctaatga tgaggctata cttcaagcag taaggcgtgt acacgataac aaaatgttcg
     4261 ataccatttc agttgaaaga aaaatacaca aacaacttgc tgatgttagc aaaatttgtt
     4321 tgggcgtgca ttgtgccaaa gaccaccaaa actactatca tgtgtgggct gtaatgcagt
     4381 cagatgccaa tggtataaaa aaaatacaag tatcaagctc taccagcttt gaacttgctg
     4441 aaaatgtagt acatcagtta ctttcttgat gttgttataa acaaatcaat gacttagaca
     4501 gttacttcgc ggttttcgct taatttttgg taatacatgt atgatataga cagcacaccc
     4561 aacgctatca gtgggtaaac cgtctcctgt acaatgccgt ccactgccca tacgcttaca
     4621 aaagcagcaa tgaaatcaat ggcaaaacct gcataaacaa attccttgat gatgttgggc
     4681 actcttggat atagcaaaac gaacccgcca attattttga acgacatgag tataaatcca
     4741 aatgcagcag gataacccaa atgttctacc ttacggataa tctctgtaga acccgcagct
     4801 ataaaaggca aaataatttc aagcacgaca atggaaatcg tcgccatcca gaatattatt
     4861 ttattttttc tcatatttta aataattatt ctgttactaa atgttgtgta gtggatgtca
     4921 tttaagttcg ttccaaatat aagtaaaata ttttaatcca ccaagaaatt aatgcagatt
     4981 atataatttt tttttaaatt gaactacaca aaccatacga atgttgatga ttttctctac
     5041 ataaaagtaa atatactata aagttaagta ggaagtaaat gtacctctac cataccacct
     5101 ttgcttatga ttcgttcacc ggcaggtata tataccaatg catcggccac tgcaaatgac
     5161 ataaggatgt ttgactcttg gccttcagtg atgcctacca tttctccttc tacaacagcc
     5221 ttaaggaaaa gagccctgtc gcctttcacc aaaaaatctt ttgacaaggg tagtctgata
     5281 cttctaagcc caattctggt atgcccgctc attttatgca aggcaggtaa tacatattcg
     5341 tagaagcaaa ccaaggcggc agccggattg ccgggtaatg caaaaaccaa tttttcacct
     5401 tgttttccga agtaaagtgg tttgccgggc ttctgtgcga ttttgtaaaa tatttttttt
     5461 acaccaagat tctgtagcac gccggctaca aggtcatatt tgccaacaga aataccaccg
     5521 gataaaatca gtacatctga ttcattaagc atttttcttg ctttgtccat cagctctgat
     5581 tcttcatcag caacggtgtc aatggtcaaa gtgtggtatc cgcattgttt gatggcagac
     5641 tgtaacgcaa acgaattgga ttcgtatatc tgcccgggtg ccaacactgc tcccggcttt
     5701 ttcagttcat tgccggtggt caataccgag atgaatggtt tgcgtgccac ttgggtgtgg
     5761 gtaatgccaa gtgtgctgag gaatccgcac atcgccggat tgataggcat gcctttgttg
     5821 gcagcccttg tacctttggt gatttcacaa gcctttagtc ggatgttgct agctttgtgc
     5881 aacgcactta aagctatttc tactttattg tttattgtgg ttgttttttc ctgcatgact
     5941 acacagtacg tgttttcggg tatcggggct ccggtaaata ttctgactgc tgttccgggc
     6001 aaaagtgctt ctgtgggtga gtcaccggcc tttgtttcac ctattatcat aaacttgacc
     6061 gcttcggttg catcaaaggg ttcgggcaaa catactgcaa aaccatccat ggccgattga
     6121 gagaatggtg gagaatcagt cggggcaatg atattttctt caagcgcgta acccgaagca
     6181 tctgctactt caacagttat tgcagatagt attcccacat tttccaacaa tagttgctta
     6241 gcttcttcta cagaaatcat tggatatttc ttttatacat tggcaacgaa attttaatga
     6301 ttcttcaggt ttttctctat ttggttcatg ttgatccgtt tgccggcaaa ataatcgagc
     6361 catctggcag ggagcttccg gatcaatttg aacatggtag cgtttttggt cactattttt
     6421 cggacctttg ggttagagtc tgtcaatgca tcatatatgg acctggaagt atagacatca
     6481 ggtagggcat tgtctatagt attttggatg aatccacttc ggtgttgagc cattttgcta
     6541 tactcatttt tgaagtcgga tgtgtcttcg gtcatggctt tatcccatat tttggttttt
     6601 attgggccgg gttctatgat acagaccttt atgccggtgt ggttcagttc tctgcgcatt
     6661 ccgtcactaa aggcttcaac cgcaaatttg cttgctgcat aaggcgacat gaatggtgtg
     6721 acgatgagcc ccgatacgga tgaaatattt acaatacggc caggtgtacc atttattttt
     6781 ccgttgagtt cgagattcgg gatgaatgcc tgtgtcacat taataaggcc aaaaacattc
     6841 acatcaaata agcgtctgag tttgtcttca gaaacatact ttaccggccc acttactgca
     6901 ataccggcat tgtttacaag tcctgctagc ggaatgtctt tcaattgatc cttgattgtg
     6961 tttatctgta ctttgtcggt cacatccatc aataaaggaa tcaggttcgg attgttcagg
     7021 gctttaatct catctgcatc ctcttgtttc ctgactgtgc cataaacaca gaatccatgt
     7081 tctgccagaa aaactgccgc acttctgcct at
//
LOCUS       DAOQQY010000137         7069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4367_length_7069_cov_6.908754, whole genome shotgun sequence.
ACCESSION   DAOQQY010000137 DAOQQY010000000
VERSION     DAOQQY010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4367_length_7069_cov_6.908754"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..533)
                     /gene="rpiB"
                     /locus_tag="PLE23_07580"
     CDS             complement(96..533)
                     /gene="rpiB"
                     /locus_tag="PLE23_07580"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HMX83162.1"
                     /translation="MTKLTIAIGCDHAGFGLKLPVIAYLEKEEYNILDMGTFSEDSVD
                     YPDFAHPVAIAVESGKADYGILICGSGNGVAITANKHTDIRAALCWNEEIAALARMHN
                     NANIIALPARFVSIEDGVQMVKTFLSTAFEGGRHERRVEKINC"
     gene            complement(530..3568)
                     /locus_tag="PLE23_07585"
     CDS             complement(530..3568)
                     /locus_tag="PLE23_07585"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMX83163.1"
                     /translation="MNSHNRITNITGWLVFIIAFAVYYASAERVGSLWDCGEFIAGAY
                     KLQVVHPPGAPLFLLIGRMFTWVASLLSNNPEDIAFSVNLLSGICSALTATFVCWVTM
                     ILGKLAMTGRNEEPDNNQVLVIAAGGVVAGLCTAFATSVWFSAVEGEVYAMSTFFTAL
                     TLWAMLKWYNAPDVPASDKWIVFAVYSAALSIGVHLLSLLTFPALALFYYFKKTKNPT
                     FKGMVVTALIGTVAIGVIQTLIIIGIPSLFSSFELLMVNSFGLPFGSGLIPLLLVLAA
                     IFYFSLRWTQRNGNGLIQRIVMSLLVAVIGFSTIGVVMIRANANPPINMNNPSDPMRV
                     VPYLNREQYGERPLLRGPQYNAKYDDIKTTDRYGRVGKHYEIVDQKIDYVYKDADKVF
                     FPRMSDYTQDRPRLYKEWTGSQSDVPSFMDNIKFFFRYQLNWMYFRYFMWNFVGRQND
                     NQGYYSWDPSSGNWISGIKFVDDARLYKSDFMPEKMKHDPSRNTYYFIPLILGLLGMF
                     YHYRKRKDDFLAILALFIITGIGIIIYSNQPPSEPRERDYVLAGSMFTFCIWIGLGVL
                     ALFDMLYQKIKLGELPAAAIASVLALSAPILMGTQNFDDHSRREIRASRDYASNFLNS
                     VDKNAIIFTYGDNDTYPLWYAQEVEGIRTDVRVVNLSLIAVDWYIDQLNRKVNDSPPV
                     KLSISPDAYRGNKRIQIPIIEEAGGPISIQQALKFIGENHPVPLSSGRSLESYVPSRQ
                     IYIPVDKEKAIRNGMISANDTAAIVSALPITLSGEFIMKDDLAVMDVIGSNFLDRPIY
                     FAVTCRQDKMLGLSDYMQLEGLALQIIPVKTASDKRFSVIGNGRVATDKLLDNVTHKF
                     RWGNFDKIQLPVSKSYQPSIQTTRYSILRGVQELIVKGEKDKAITLLDTYFKAFPDMN
                     FPYDFDILPFLQSYVEAGAYDKAKTQMKILAKNTLASLKFYTSLSTTDLERGFSSEYG
                     YTLKTVDEMQEIAKKGKDEAYAKELHDMFSKYYVKKEPVK"
     gene            3727..3882
                     /locus_tag="PLE23_07590"
     CDS             3727..3882
                     /locus_tag="PLE23_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83164.1"
                     /translation="MRFLLLILSIYLLYKLFAAPVISMNKQYQRKNERQKDDTKNQEY
                     TDYEEVE"
     gene            3883..4599
                     /locus_tag="PLE23_07595"
     CDS             3883..4599
                     /locus_tag="PLE23_07595"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HMX83165.1"
                     /translation="MSTNENFKIGDLILANNHRFIICDKPAGMPVQEDKTGDKSLLSL
                     AEIFCKTRLHPVNRIDRPVKGLVIFAKDGKNATLLSEMLADGRIEKWYLGITRNKPEK
                     ESATLEQWLIHDKKTNKSYIYDTEMPGSKPACLDYNIIGSSTNYHYWLVRLHTGRHHQ
                     IRAQLASLNCPVKGDVKYGDKRANPDKSISLLAWKLNFINPLHPKVTEISSTLPDDAL
                     WNHLNENILPVWIAKLSELK"
     gene            4569..5609
                     /gene="thiL"
                     /locus_tag="PLE23_07600"
     CDS             4569..5609
                     /gene="thiL"
                     /locus_tag="PLE23_07600"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMX83166.1"
                     /translation="MDSEVKRTEISTLGEFGLIDKLTKGKPLVQASSLTGIGDDAAVI
                     NHAGEPTVISTDILIEGIHFDLMYMPLKHLGYKAVAVNLSDIYAMNATPTQITVSIAF
                     SNRFPIEAIEEIYEGIYSACKNYNVDLIGGDTSSSNKGLIISVTAIGTAKAGKLCYRN
                     GAKPGDLICISGNLGAAYLGLQLLEREKQLFLENQNVQPDLEDQVYVVGRQLKPEPRF
                     DIIETFEKAGLVPSAMIDISDGLVSEIFHICRQSGTGAYIEESGVPIHPDAEMLALRF
                     NLDPITCALYGGEDYELLFTVDPKDLDKVKYIPDVYIAGEITEASDGIKLHTKGGNIH
                     DLKDKGFNHFSA"
     gene            complement(5599..6294)
                     /gene="trmB"
                     /locus_tag="PLE23_07605"
     CDS             complement(5599..6294)
                     /gene="trmB"
                     /locus_tag="PLE23_07605"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HMX83167.1"
                     /translation="MSRRNKLFKFAEMLTFPNVFENPNFHNPHLTGLNGEAVEMKGKW
                     NENYFSNNHPLVLELACGRGEYSFALGQRMPEKNFIGVDIKGARIYQGAKKVLDAGMQ
                     NVAFLRTKIELINHFFSPSEISEIWITFPDPFLRDSKANKRLTSPRFLDIYRKILKPE
                     GIIHLKTDDPKLYEYTLEVLSESEDCEIIHQDDDIYSKALYDETLEIKTYYEIRHLAD
                     NRTIKYVRFKFKH"
     gene            complement(6304..6810)
                     /locus_tag="PLE23_07610"
     CDS             complement(6304..6810)
                     /locus_tag="PLE23_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83168.1"
                     /translation="MKLIYGLILLCLLNIQCKQDDRILFDFYYQQDLTLPAGLSFLQT
                     HYFQFDDIPTNFSNLLKAKGYTIKDIAKVSPGSGYMLALDSGVNLDFIQEVSIMISST
                     GQFNKEVLYSVQVPLNAGNHIDLAGTLVDALDVLSKDRFNLRISFKLRANCPETFDAR
                     FTIKFLAL"
BASE COUNT         2170 a         1431 c         1406 g         2062 t
ORIGIN      
        1 tttggtgttg actgagcgtc agtacttaga ggaactgcca gcaatatgaa cagtaccaga
       61 tgggaaaatt tgtgaatgtt catttcgtgg tattatcaac agtttatttt ttcaaccctt
      121 ctttcgtgcc ttccgccctc gaatgcagtt gacaaaaaag tttttaccat ttgtacacca
      181 tcttcaatag atacaaacct ggccggcaat gctatgatgt tggcattgtt gtgcattctt
      241 gcaagtgctg ctatttcttc attccagcaa agtgcagctc tgatgtcagt atgtttattg
      301 gcggtaatgg ctactccatt gccactacca cagatgagta tgccataatc ggctttgcca
      361 ctctcgaccg ctattgctac cggatgtgca aaatcaggat aatctacaga atcttcagag
      421 aaagtaccca tatcgagaat attgtattct tctttttcga gatatgcgat aacaggtaat
      481 ttcaggccaa atccggcatg gtcacatccg atagcaatgg tcaattttgt cattttacgg
      541 gttctttttt tacataatat ttactgaaca tatcatgaag ttctttggcg tatgcctcgt
      601 ccttaccttt ctttgcaatt tcctgcattt catcaacagt cttcagagta taaccatatt
      661 cgctcgagaa tcctctttct aaatcggttg tagagagtga tgtatagaat ttcagactgg
      721 ccagcgtatt ctttgccaga atcttcattt gagtttttgc tttgtcataa gcacccgctt
      781 ctacatagga ttgaaggaat ggcaggatgt caaagtcata agggaaattc atatcgggga
      841 aagctttaaa ataagtatcg agcaatgtga tagccttatc tttttcacct ttaactatca
      901 actcctgaac accacgtaga atagaataac gtgtggtctg tatgcttggc tgatagcttt
      961 tagacacagg gagttgaatt ttatcaaaat tgccccatct gaacttatga gtaacgttat
     1021 ctaagagttt gtcagtagct acacgaccat tgccaatgac actaaatcgt ttgtcgcttg
     1081 cggtttttac cggaataatc tgtagtgcca gtccttcaag ctgcatatag tcagataggc
     1141 caagcatctt atcctgtcgg caagtgactg cgaagtatat aggacggtca aggaaatttg
     1201 agccaatgac gtccatgaca gccaaatcat ctttcataat aaattcgcct gataaagtaa
     1261 ttggcaaggc tgaaacaatt gcagctgtat cgtttgcaga gatcatacca ttacgaatgg
     1321 ccttttcttt gtcaaccgga atgtaaattt gcctagaagg aacataactt tcaagacttc
     1381 tgcccgagct aagcggcaca ggatgattct cgccaataaa ttttaaagct tgttggatac
     1441 ttattggtcc cccggcttct tctataatcg gtatttggat gcgtttgttg ccacggtatg
     1501 catctggcga aatacttagc ttgacaggtg gggagtcgtt cacttttctg ttaagttgat
     1561 caatatacca gtccacagct ataagactaa ggtttacgac ccttacatct gtacgaatgc
     1621 cttctacttc ctgtgcatac caaagcgggt atgtatcatt atcgccgtag gtgaaaataa
     1681 tagcgttttt atctacagag ttgaggaaat tgcttgcata atctcttgaa gcacgaatct
     1741 cccttcggct gtggtcatca aagttttgag tgcccatcaa tattggagca gacagtgcca
     1801 gaacacttgc tatagcagca gccggcaatt ctccaagttt gattttttga tacaacatat
     1861 cgaacaatgc cagtacacct aagcctatcc agatgcagaa tgtaaacatc gaacctgcga
     1921 gtacatagtc acgttcacgt ggctcacttg gtggttggtt ggaataaatt ataataccga
     1981 taccggtgat gatgaatagt gccagaatag caagaaaatc gtctttacgt tttcggtaat
     2041 gataaaacat ccctagcaga ccaagtatca gaggaataaa atagtaggta tttctacttg
     2101 ggtcatgctt cattttttcg ggcataaaat cagatttgta caaccttgcg tcatcaacaa
     2161 acttaatccc cgatatccag tttccactac tcggatccca tgaataatat ccctgattgt
     2221 cattttgtct gcccacgaag ttccacataa aatatctgaa gtacatccag ttgagttggt
     2281 atcggaagaa gaatttgata ttatccataa aactcggaac atcagactgg ctaccggtcc
     2341 attccttata caatcttggt ctgtcttgag tatagtcaga catacgcggg aagaacacct
     2401 tatcggcatc cttatataca taatctatct tttggtcaac gatttcataa tgcttgccta
     2461 cacgtccgta tcggtcagtg gtcttgatat catcgtattt tgcattgtac tgaggtcccc
     2521 tcaaaagagg tctttcacca tattgttcac gattgagata tggtaccact ctcattgggt
     2581 cgcttggatt gttcatgtta ataggcgggt tggcatttgc tctgatcata acgacaccta
     2641 tggtcgaaaa accaataact gctaccagca ggctcatgac aatacgctgt atcaatccat
     2701 taccattgcg ttgagtccat cttagagaga agtagaaaat agcagctaaa acgagtaaaa
     2761 gagggataag gccagaacca aaaggaagcc cgaacgaatt aaccatcaat aattcgaagg
     2821 atgagaacaa actcgggata ccaataataa tcaatgtttg tataacacca atggctacag
     2881 taccaatcag cgcagttacc accattcctt tgaaagtagg gttctttgtt tttttgaaat
     2941 aatagaaaag tgccaatgca ggaaaggtaa gcaaacttaa caagtgtaca cctatagaaa
     3001 gagccgctga ataaacggca aaaacaatcc atttatcaga agccggtaca tcgggagcat
     3061 tgtaccattt taacattgcc caaagggtaa gagctgtaaa gaaagttgac atggcatata
     3121 cttcaccttc tacagccgaa aaccatacag acgtggcaaa agcagtacac aagcctgcaa
     3181 ccacaccacc agcagcaata accagtacct gattattatc tggttcttca ttgcgcccgg
     3241 tcatggccag tttgccgagt atcatggtta cccaacatac aaaagtagcc gtaagtgcag
     3301 agcatatacc cgacaataag ttgactgaaa atgctatgtc ttcaggatta ttggaaagca
     3361 agcttgctac ccaagtaaac atacgcccga taagcaaaaa cagaggtgct cctggtgggt
     3421 ggacaacctg tagcttgtac gccccggcta taaattcgcc gcaatcccaa agactgccta
     3481 cccgctccgc tgatgcataa tatacagcaa aagcaataat gaaaaccaac caaccggtaa
     3541 tgtttgttat tctgttatga gaattcataa tgttgtctga aatttttatt aaaacacttt
     3601 ttctaattaa aaaacaaaac gcaaatctaa taaaatattg gtgttttgct tttgtttaac
     3661 tacattgaaa agaaattatt gtcaaaacaa ggattatcaa gtacctttat gtcgaaaatt
     3721 tagacgatga ggttcttatt actgatactt tcaatctatt tactctacaa gttgttcgca
     3781 gctccggtca tttcaatgaa caaacaatac caaagaaaaa acgagcgcca aaaagacgac
     3841 accaaaaatc aagaatatac cgactacgaa gaagttgaat aaatgagtac aaacgaaaat
     3901 tttaaaatcg gagatcttat tttagctaat aaccatagat ttataatctg tgacaaacct
     3961 gcaggtatgc ctgtacagga ggacaaaaca ggtgacaaat ctttgctttc gcttgcagaa
     4021 attttttgta aaacaagatt gcaccctgtc aatagaattg acagacctgt aaaaggactt
     4081 gtaatttttg caaaagacgg taagaatgca acattgctga gtgaaatgct tgctgatggc
     4141 cgtatcgaaa aatggtatct gggcataacg cgcaataaac ctgaaaaaga atctgccaca
     4201 ctcgaacaat ggctcattca tgacaaaaaa actaataaat cgtatatata cgatacagag
     4261 atgccgggtt caaaacctgc ctgcctggac tacaacatta ttggctcttc taccaattac
     4321 cattactggc tggtgaggct gcataccggc cggcaccatc agataagagc tcaactcgca
     4381 tcacttaatt gtcctgtaaa aggtgatgtt aaatatggcg acaaacgtgc caatcctgat
     4441 aaaagtataa gtctgctggc ctggaaatta aattttataa atccactgca tccgaaagtg
     4501 accgaaataa gctcaaccct accggacgat gcgctgtgga atcaccttaa tgaaaatata
     4561 ttacccgtat ggatagcgaa gttaagcgaa ctgaaataag tacgctgggc gaatttggcc
     4621 taatagacaa actgacaaaa ggtaaaccgc ttgtgcaagc atcttctctt accggtatag
     4681 gtgatgatgc ggcagtcatc aatcatgccg gcgaaccaac tgttatatca acggatattc
     4741 tgatagaagg catacacttc gaccttatgt acatgccact caaacacctc gggtacaagg
     4801 ctgtggcagt caacttgtct gacatatatg ctatgaatgc cacgcctaca cagataacag
     4861 tgagtattgc attttcaaac cgtttcccaa tagaggccat tgaagagata tacgaaggca
     4921 tttattcagc ctgcaaaaat tacaatgttg accttattgg gggagacaca tcttcttcta
     4981 acaaaggcct tattatatcg gtcaccgcta tcggtacagc taaggcaggt aagctatgtt
     5041 accggaatgg tgcaaaaccc ggcgacctta tctgcataag tggcaatctg ggagctgctt
     5101 atcttggcct tcagttgcta gaacgtgaaa aacaactttt tcttgaaaat cagaatgtac
     5161 aacctgacct tgaagaccaa gtatatgtgg taggcagaca gcttaaaccg gagcctcgat
     5221 ttgatattat tgaaaccttt gaaaaggcag gtttggtgcc gagtgctatg atcgatattt
     5281 ctgacgggct tgtttctgaa attttccaca tttgccggca gtcgggtaca ggtgcttaca
     5341 tagaggagtc gggcgtacca attcatccgg atgcagagat gcttgcactt aggtttaatc
     5401 ttgacccaat tacttgcgca ttatatggtg gcgaagatta cgaattatta tttacagttg
     5461 atccgaaaga cctcgacaag gtaaaataca taccagatgt atatatagcc ggcgaaataa
     5521 cggaggcatc ggatggaatc aaattacaca cgaaaggtgg caacattcat gatttaaaag
     5581 acaaaggatt taatcatttc agtgcttaaa cttaaaacgg acatatttga tagtcctgtt
     5641 atctgccaga tgcctgattt cgtagtaggt tttaatctct aatgtttcgt cgtacaatgc
     5701 ttttgaataa atatcatcat cttgatgaat gatttcgcaa tcttctgatt cgctcagtac
     5761 ttcaagtgtg tattcgtaaa gtttagggtc gtcggttttt agatgtatga tgccctccgg
     5821 ttttaaaatt tttcggtata tatcaagaaa gcgaggtgaa gtgagccgtt tatttgcctt
     5881 gctatctcgt agaaaaggat ctggaaatgt tatccatatt tcggagattt cggacggtga
     5941 aaaaaagtga ttgatgagtt cgattttggt acggaggaaa gccacattct gcattcctgc
     6001 atcgagtact ttttttgctc cctgatagat acgggctccc ttaatatcaa caccgatgaa
     6061 atttttttca ggcattcgct gtcctagggc aaaactgtat tcgccacggc cacatgccaa
     6121 ctcgagtacc aatgggtggt tgttggagaa ataattttca ttccattttc ctttcatttc
     6181 cactgcctca ccattaagtc cggtaaggtg aggattgtga aaattaggat tttcaaatac
     6241 attggggaag gtcagcattt ctgcgaactt aaaaagtttg tttcttctgc tcatgaaaat
     6301 aagttataag gcgagaaatt taattgtaaa ccgtgcatca aatgtctcag gacaattggc
     6361 tcgtagtttg aagcttatgc gcaggttaaa tctgtctttg ctaagaacat caagtgcatc
     6421 cacgagtgtt ccggccagat cgatgtggtt tccggcattg aggggtacct gtaccgagta
     6481 taatacttct ttgttaaact gcccggtact agatatcatg atgctcactt cctgaatgaa
     6541 gtcgagattg acacccgaat ctagagcaag catatacccc gaacccgggc tcaccttggc
     6601 aatatcttta atagtgtaac cctttgcttt tagtagattg ctaaagttgg taggtatatc
     6661 atcaaactgg aaataatgtg tctgcaaaaa actaagtcct gccggcaatg tcagatcctg
     6721 ctgataataa aagtcaaaaa gtatgcgatc atcctgcttg cactgtatgt tgagcaaaca
     6781 aagcaatatc aaaccgtaaa tcagtttcaa tttttaaaaa attttcacaa tagtaacgca
     6841 gtattttata atttgagtac ttttttgtga agtctttttg taggcgtatc tatttcaatg
     6901 atatacatac ctgctttagc agagattccg gacattggat aaccacttcg gactacttta
     6961 tcattaaaaa aattttactt tatttacctt ttagactaca tttgaaaaaa aataatagaa
     7021 catgggagag cagagtataa ccttaaatcc tgaccaattg tacatgcat
//
LOCUS       DAOQQY010000138         7019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4430_length_7019_cov_5.627513, whole genome shotgun sequence.
ACCESSION   DAOQQY010000138 DAOQQY010000000
VERSION     DAOQQY010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7019
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4430_length_7019_cov_5.627513"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            611..685
                     /locus_tag="PLE23_07615"
     tRNA            611..685
                     /locus_tag="PLE23_07615"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:646..648,aa:Pro,seq:tgg)
     gene            905..2677
                     /locus_tag="PLE23_07620"
     CDS             905..2677
                     /locus_tag="PLE23_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HMX83169.1"
                     /translation="MHLPSISVLLTSFKDAFIRFPMSMLFAMLTCILSVYMVEYDINE
                     KSPVFKLLTMSIIGVPLMISAKIFSERFNRNGIAKWIAYALGLVVLLLYMYFIAPDFE
                     SSKVVKPIRFVSVLIFSHLLVSVAPYLRVGQVEDFWEYNKQIFVNWLFGAAYGLIIFL
                     GIAVAFLAVDNLFGVHIEFDWYVEIFLIIASVFHPVYFLDHFPENFHRIADDGKYSKV
                     ILNIVKFILIPLSLLYITILYLFSIKIGVEMALPKGWVASLVIGFSITGMLTYLLNYR
                     LPDVFPQESPLLAWFKKWFFYLMVPLVMLLFVAIIKRVMDYGFTPPRYFVMLTAVWLG
                     CILLYFIISKKDKIKLIPMSLIGFLAVGSLSPIDAFRVSAQSQYNRLMRMFEEKNLVI
                     DEIIHVDTTKLMLSVPQQERAASMVQVLDAMNSLNNLNEHLHDKIHQDSLNSANAGLM
                     MMNKLGLTKAGHVIQPEEFYTYSVNVNDALHVEGYSVLLPLNIFSGEKRKDGFEFSDD
                     QTKILWKKNNQVLATLEFKELMKNLAEKYGNGKYDIPRAELSFTFENEQYRMKLMLNA
                     VSFGLIGTERRVNSIDGYFLYAEK"
     gene            complement(2685..3638)
                     /locus_tag="PLE23_07625"
     CDS             complement(2685..3638)
                     /locus_tag="PLE23_07625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HMX83170.1"
                     /translation="MKFRTELTPPPASAQIKPGDKILLAGSCFAENIASKLTSLRWQI
                     LLNPHGILFNPASVDHCIQDIGEKKSYTPADLFEADGTFHSFRHHGMYSGTDASNVCS
                     NINHHISLASNYIKEAQWCILSYGTAWIYRLLSSGEIVSNCHKVPQKKFTKELLSVLQ
                     LTEIIQSTIIALQNMNPDIRIIITVSPVRYLRDGFVENARSKAHLVTAVHEVLERQRG
                     VYYFPAYELFLDDLRDYRFTKEDLVHPNNQAIDYIWDKFEKSYFTPEALTFNTDMQTL
                     ANMKNHRPLYPDTRHYQQFQNKISELEMHLTKKYPFLIGKN"
     gene            3778..4425
                     /locus_tag="PLE23_07630"
     CDS             3778..4425
                     /locus_tag="PLE23_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMX83171.1"
                     /translation="MNFKLIFCMIGFMLIARNGSLQSQTSDTSIVQLSGLVLDGSGEI
                     LLPVAYTNVFIKGRSSGTFADLKGFFSIVARKGESVRFTSVGYKTVEYQVPKVLASDR
                     YSIVQLMTKDTINLPETIIFPWPSRERFTTDFLAMDVSHAIQEKAKENLARESLEKLR
                     FEVPKDAREYTSYYSQQFNQKYYYMGQTPPMNIFNPLAWAQFFKEWKQGKYKKKK"
     gene            complement(4435..>7019)
                     /locus_tag="PLE23_07635"
     CDS             complement(4435..>7019)
                     /locus_tag="PLE23_07635"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HMX83172.1"
                     /translation="YANGKPFIKQAIDKKSEKASPGYYSVLLPDAGITAELSCTDRVG
                     WHRYSFNDAAQAKIFIDLEHRDKLLEASLQQTGMKEITGHRISEGWARRQHLYFVMQF
                     SENIEITDKSAGPYSLILKPEVKTGVPLIIKVGISAVSIERARKNLNAESPGWDFDAV
                     VKAADQRWEKELSKIVVKSDVRTDLVNFYTSMYHLHIAPNIFCDVDSMYLAMDGKTYK
                     AEDFTPYTVFSLWDTYRAAHPLFTIIDRKRTEDYIKTMLDHYKKAGHLPVWELDGNET
                     WCMIGYHSVSVIADAYVKGITNFDTKLALEAMIESANAKRFGIDHYVANGYVPAEKSG
                     ESVSKTLEYAYDDWCIAQFANAIGNMEVYNQYMKRAQYYKNLMDPKSGFMRARSNNIF
                     LEPFLPTDINMHYTEGNSWHYSFTAVQDIDNFKRFLGGEKALEQKLDELFNNKNKLTG
                     REQSDVTGLIGQYAHGNEPSHHIAYLYNQTASPWKTQELTRKIVTELYKNDAVEGLCG
                     NEDCGQMSAWYIMSALGLYPLSPGNDYFELTSPLFDEANIRLENGKTFRLLTKGNAHF
                     NPYIQEVTIAGRPLDRSYIMYSEILNAAPLTFLLDKTPNKNLWTKAENRSPSAITKNK
                     IVPLPFVSAPQTVFVNNTSFSLHDLEPAASLWYSFDKEALISKYIKYTKPVVVEDSKI
                     VYYYAKMPDGSISNVVSTEFRKLDPRIKVLSHSTYSNQYPGGGDYALVDLVKGGNDYR
                     SLAWQGYEGNDLAYEVDLGSIKNVNSIGLSVLQDQKSWIFFPVSLSIQTSTDGVNYTD
                     YGTVINQIPADRDGAILQELTVKKEQSVRFVRVNAKSMGKIPEWHLGAGNKPWIFADE
                     IVIK"
BASE COUNT         1937 a         1290 c         1448 g         2344 t
ORIGIN      
        1 ttctgtgttt gacgaaaaag tcagatacaa aagacaggaa aaatatttaa cagagagaaa
       61 tattttattt tttattttct aaaattttat tttcgcttgt aagagtagag tatatcgagt
      121 ttataaaagc taattagaat ttatttttga cttttaattt tttttattaa tattttattt
      181 atactattaa gaaattttta gtaattttat tgtttagttt aaaaatctaa ttattagata
      241 tttgaatttt tttcaatttt tatcagatta gtatttttct aaatataagg ttatttttat
      301 gtttaattga atactaaatt taaaagtttt cttctttaag aagaataatt tatattttaa
      361 acagtgtttt ttatttttaa aaagtagctc agtttacagt gttagatgat tcctcgaata
      421 cgataattgt ttctgaaaaa ttaaattcac attgtaaaca gcttaataag catattattt
      481 ataaataaac tctataataa taataatcat atataattaa aaagtttcaa aattgaatct
      541 aaaatttagt cacaaaaaaa ttagaaaaaa cggaacataa ccataaaatg ttcgtttgat
      601 ataaaagttt cggggtgtag cgcagctcgg ttagcgcacc tgctttggga gcagggggtc
      661 ccaagttcga atcttggtac cccgacttca ttcaaaagag gaatagcaat gttcctcttt
      721 ttttattttt ggctgtttga taaatttttc ctacgtgtgt tattcagtat tttgcaaatt
      781 gtgtttggcc tcctaaatac ctatcagaga tattttagca ttttaataag ttttttttct
      841 catgagaaat caatactgaa gcattttatg tattttgcag cattaacgac tatcggataa
      901 ctgtatgcat ttgccatcta tttctgtatt gctcacgagc ttcaaagacg ccttcatccg
      961 attcccgatg tcgatgttgt ttgctatgct cacctgtatt ttgtcggtct atatggtcga
     1021 atacgacatt aacgaaaaat caccggtatt caagctgttg accatgagta tcataggcgt
     1081 accgctgatg attagtgcaa agatattttc cgagcgtttc aatagaaatg gtattgccaa
     1141 gtggattgcc tatgctttgg gtcttgtagt tttgttgctc tacatgtatt tcatcgctcc
     1201 cgattttgaa tcgagcaagg tcgtaaagcc aataaggttt gtctctgttc ttattttttc
     1261 tcacttactg gtatcggtgg ctccttactt gcgggtaggt caggtcgaag acttttggga
     1321 gtataataaa cagatatttg tcaattggtt gttcggtgcg gcttatgggc tgattatttt
     1381 ccttggtatc gcagttgcct ttcttgcagt agataatctt tttggtgtgc atattgagtt
     1441 cgattggtat gtagagattt ttctgatcat tgcttcggta tttcatccgg tttatttctt
     1501 agaccatttt ccggagaatt ttcaccgtat tgccgatgat ggaaagtatt caaaggtcat
     1561 tctcaatatt gtaaaattta tattgatacc gttaagtttg ctatatatca ctatacttta
     1621 tctttttagt ataaaaatag gagtagagat ggctttgccc aaaggttggg tggcaagctt
     1681 ggtaattggc ttctctatca caggcatgct tacttatttg ctcaattaca gactaccgga
     1741 tgtttttcct caagaaagcc ctttgttggc ttggtttaag aaatggtttt tttacctgat
     1801 ggtacctttg gtaatgttat tatttgtagc catcatcaag cgtgtgatgg attatggttt
     1861 tacaccaccc agatattttg tcatgctcac tgcagtatgg cttggatgta ttctgcttta
     1921 ttttattatt tcaaaaaaag acaaaattaa actcattcct atgagtctta ttggttttct
     1981 tgcagtaggc agccttagcc caatagatgc cttcagggtt tctgcccaga gtcagtacaa
     2041 tcgtttaatg agaatgtttg aagaaaagaa tcttgtaata gacgaaatca ttcatgtcga
     2101 tactacaaag cttatgctca gtgtgcccca acaagaacgt gctgcatcaa tggtgcaggt
     2161 acttgacgct atgaattcac ttaataatct gaatgaacat cttcatgata aaatacacca
     2221 ggattcactc aactctgcta atgccgggtt gatgatgatg aacaagctcg ggcttactaa
     2281 agcaggccat gtaatacaac cggaagagtt ttatacatat agtgtaaatg taaatgatgc
     2341 cctgcatgtg gaaggatatt cggtattgct tccgctgaat attttctcgg gcgaaaaacg
     2401 aaaagacggt tttgaatttt cagatgacca gacaaaaata ctttggaaaa aaaataacca
     2461 ggtgcttgca acgctcgagt ttaaagaatt gatgaaaaat cttgccgaaa agtatggtaa
     2521 tggtaaatac gatataccaa gagctgagct tagctttaca ttcgaaaatg aacaatacag
     2581 aatgaaactt atgctcaatg cggtgtcctt tggtttgata ggtactgaac gtcgggtaaa
     2641 cagcatcgac gggtatttct tatatgccga aaaatgatcg tcagctaatt tttaccaata
     2701 agaaatgggt attttttcgt gagatgcatt tcgagttcag atattttatt ctgaaactgc
     2761 tgataatgtc ttgtatcggg gtacaagggt cggtgatttt tcatattggc cagtgtctgc
     2821 atatcagtat tgaaggtcaa agcctcaggt gtaaaataac ttttctcaaa tttgtcccag
     2881 atataatcta ttgcctggtt gttggggtgc acaaggtctt cttttgtaaa gcgataatcg
     2941 cgtaggtcgt ctaagaataa ctcatacgcc ggaaaataat aaacaccacg ctgccgctca
     3001 agtacttcat gtacagcggt gaccagatgc gccttgcttc tggcattttc tacaaaacca
     3061 tctctcagat atcttacagg acttactgta atgataatcc ttatgtcggg attcatgttt
     3121 tgtaaggcaa taattgttga ttgaataatc tctgttagtt gcaatacact tagcaattct
     3181 ttggtgaatt ttttttgagg taccttgtgg caattactca caatctcacc agaagaaaga
     3241 agcctataaa tccacgcagt gccgtagctt agtatgcacc attgtgcttc ctttatataa
     3301 tttgaggcaa ggctgatatg atggttgata ttgctgcata cgttggaggc atcagtacct
     3361 gagtacatgc catggtgacg gaaactatgg aaagttccat ctgcttcaaa taagtcggcc
     3421 ggtgtgtaac ttttcttttc accaatatcc tgaatacaat ggtcaacaga agcaggattg
     3481 aacaaaatgc cgtgtggatt gagcagtatt tgccaccgaa gtgaggtgag tttacttgct
     3541 atattctctg caaaacaaga tccggccagt aatattttat caccgggttt tatttgggca
     3601 gatgccggag gcggtgtcaa ttcggtcctg aatttcattt gtgattaaac ttttagggac
     3661 attttttaat ctgaataata agaatagtaa gtaaaaagcg tattatatta atgttaagaa
     3721 aactttattg accgacaaat atatcaacta aacgttttga aagtgtaact ttaccaaatg
     3781 aacttcaagc tcattttttg tatgattggc tttatgctca ttgctcgtaa cggaagctta
     3841 caatcacaga cttctgatac gagtattgtt cagctatcag gtcttgtgct ggatggttca
     3901 ggcgaaattc tgttgccggt ggcttacacc aacgttttta tcaaaggtag aagctccggt
     3961 acatttgccg acttgaaggg ctttttctcc attgtagcac gtaaaggtga gtctgtaagg
     4021 tttacaagtg taggttataa gacggttgag tatcaggtgc caaaggtact cgcatctgac
     4081 cgttactcga ttgtgcaact gatgaccaaa gataccatta atctcccgga aacgatcata
     4141 tttccatggc cgagtcgtga gcgttttaca acagatttcc ttgccatgga tgtttcgcat
     4201 gctatccaag agaaggcgaa ggaaaatctt gccagagaaa gcctcgaaaa gctcaggttt
     4261 gaagtaccca aagatgcacg cgaatatact agctattatt ctcagcagtt taaccagaaa
     4321 tattactaca tgggccagac accacctatg aatattttta atccgcttgc ctgggcacaa
     4381 ttcttcaagg aatggaagca aggaaagtac aagaagaaaa aataatacaa ctgcttactt
     4441 aattacgatt tcatctgcaa atatccatgg cttattacct gcgcctaagt gccattcagg
     4501 tattttgccc attgacttgg cattgacacg tacaaacctc acagactgct ctttctttac
     4561 tgttagctct tgcaaaatgg ctccatccct gtcggccggt atttggttta tgaccgttcc
     4621 atagtcggta tagttaacac catctgtact ggtctgaatg cttagggaca ccgggaagaa
     4681 tatccacgac ttctggtctt gcagaacact cagaccaata gagtttacat ttttgatgct
     4741 acccaagtca acttcgtaag ccagatcatt gccctcatag ccttgccatg ccaggcttct
     4801 gtagtcatta ccacctttga ccagatctac aagcgcatag tcgccaccac ccgggtattg
     4861 gttgctgtat gtcgaatgcg acagtacctt tattctgggg tcgagtttgc ggaattctgt
     4921 actgacgaca ttgctgatag agccgtcggg catttttgca taataataga cgatcttgct
     4981 gtcttcaacc actaccggtt ttgtgtattt tatatattta ctgatgaggg cttctttgtc
     5041 aaagctatac caaagacttg ctgctggctc aaggtcatgt aggctgaagc ttgtgttatt
     5101 gacaaaaact gtctgaggtg ccgaaacaaa aggtagtggc acgattttgt ttttggtgat
     5161 agctgacggg cttctgtttt cggcttttgt ccatagattt ttatttggtg ttttgtcaag
     5221 caagaaagta aggggggcag cattcaggat ttcagaatac ataatgtaag acctgtcaag
     5281 cggccttcct gctatcgtta cttcctgaat atatggattg aaatgtgcat tgcctttggt
     5341 gagcaagcgg aatgtcttgc cattctcaag cctgatattt gcttcatcaa acaagggtga
     5401 ggtaagctca aaataatcat tgccagggct cagcggataa agcccaagtg cactcatgat
     5461 ataccatgcc gacatttgtc cgcaatcttc attgccacat aagccttcta cagcgtcatt
     5521 tttgtagagt tcggtcacta tctttcgtgt cagttcttga gttttccaag gagaggcggt
     5581 ctggttgtaa agataagcta tgtgatgact tggttcattg ccatgagcat actgcccgat
     5641 cagaccggtt acgtccgatt gttcacggcc ggtaagtttg tttttattat tgaacagttc
     5701 gtccagtttt tgttcaagag ccttctctcc acctaggaat cgcttgaaat tgtcaatatc
     5761 ctggacagcc gtaaaactgt agtgccacga attgccttcg gtataatgca tattgatgtc
     5821 ggtaggcaaa aatggttcga ggaagatatt gttggaacgg gcgcgcatga accccgattt
     5881 cggatccatc aggtttttgt aatactgtgc acgtttcatg tattggttat atacttccat
     5941 gttgccaatg gcgtttgcaa actgtgctat acaccagtcg tcataggcat actccagtgt
     6001 ttttgagaca gattcgcccg atttttcggc aggtacataa ccgttggcca cataatgatc
     6061 aataccaaac cttttggcat tggccgattc gatcatggct tcgagtgcca gtttggtatc
     6121 aaagttggtg attcccttga cataagcatc tgcaatgacg ctgacggagt gataaccaat
     6181 catacaccat gtttcattgc catcgagctc ccatacgggc agatgtccgg cttttttgta
     6241 atggtcgagc atggttttga tatagtcttc tgtccttttt ctgtcaataa tggtaaaaag
     6301 aggatgagca gcacggtagg tgtcccacag agagaagacc gtgtaaggtg taaagtcttc
     6361 ggccttgtag gtttttccat ccattgcaag gtacatactg tcaacgtcac agaagatatt
     6421 gggcgctatg tgcaagtggt acattgatgt gtaaaaattt accaggtctg tacggacatc
     6481 tgatttaaca actatttttg aaagttcttt ttcccaacgt tggtcggcgg cctttacaac
     6541 tgcatcaaaa tcccaacccg gtgattcagc attcagattt tttctggctc tttctatact
     6601 tacagccgat attcctactt ttattatcaa tgggacaccg gttttgactt cgggttttag
     6661 tatcaatgaa tatggtccgg ctgatttgtc ggtgatttcg atattttcgg aaaattgcat
     6721 gacgaaatac aaatgttggc gtcttgccca gccttctgaa atacggtggc cggttatttc
     6781 cttcattccg gtttgttgga gggatgcttc taagagttta tcgcgatgct cgaggtcgat
     6841 gaaaattttt gcctgtgcag catcattgaa tgaatacctg tgccagccaa ctcgatcggt
     6901 acaactgagt tctgctgtaa tgccggcatc cggaagcaat acgctataat atccgggtga
     6961 tgctttttcc gatttcttgt ctattgcttg ctttataaat ggctttccgt ttgcatatt
//
LOCUS       DAOQQY010000139         7018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4432_length_7018_cov_6.983197, whole genome shotgun sequence.
ACCESSION   DAOQQY010000139 DAOQQY010000000
VERSION     DAOQQY010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4432_length_7018_cov_6.983197"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLE23_07640"
     CDS             complement(<1..313)
                     /locus_tag="PLE23_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83173.1"
                     /translation="MRIFLFGIMVFLSAIASAQSDKVVKWTATAVKESDNLYTLTLTA
                     RIEKPWYVYSQFLADDGPVKTSFRLENAEKFDLSEQPTEEGNKKAGFDEIFGMELVKF
                     SN"
     gene            complement(471..1178)
                     /gene="purQ"
                     /locus_tag="PLE23_07645"
     CDS             complement(471..1178)
                     /gene="purQ"
                     /locus_tag="PLE23_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMX83174.1"
                     /translation="MKFGVVVFPGSNCDDDMIHVLGDVMKSEVVKVWHKDTGLPGFNT
                     SDCIVLPGGFAYGDYLRCGALAGLSPIMSAVKDFAAKGGMVVGICNGFQVLCEAGLLP
                     GVLLANSSQKFICKNIHLKIENNETPFTRLTSKGDVLKIPIAHAEGRYYADENILGQL
                     EMNNQVLFRYCSPTGEVADEYNPNGASRNIAGICNEGRNVFGMMPHPERASEEILGNT
                     DGRILFESLLNHVLESA"
     gene            complement(1188..2012)
                     /locus_tag="PLE23_07650"
     CDS             complement(1188..2012)
                     /locus_tag="PLE23_07650"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83175.1"
                     /translation="MPENKYWLLVIQIIIVVLQAGILNATINKTRMMRDINLFPGFVW
                     VVTVSLFPEYLCDTTMLFANLFLLLSVRELFGVYKKNSVAGGIFNAGLFLSMSVFFYP
                     GFIFFTIAIILGLQILRAPKIKEYLMLCSGMLVPVILLTVLFFWNDSLHLIRQAQLDV
                     FMFNFKFEPQSNGIYKLGFIILSILLCLLGYSRIIFKQSIQNIKYIEILYLLLFIGIS
                     MTFFIKDRNIEQYLSILLPVSVIGGLILYSISSSSAEMIHLLLIFIVFFWQFAPNF"
     gene            2623..3597
                     /locus_tag="PLE23_07655"
     CDS             2623..3597
                     /locus_tag="PLE23_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMX83176.1"
                     /translation="MALSIDSIQQPINAELKIFETKFRDSMKSHVPLLDKITYYIVKR
                     KGKQMRPMFVFLSARILGNPSESTYHAASMVELLHTATLVHDDVVDDSFERRGFFSIN
                     ALWKNKIAVLVGDYLLSRGLLLALENKEFQLLSILSTAVKMMSEGELLQIEKARNLDI
                     KEEIYYEVIRQKTASLIAAACSAGAASVTEDESVIERFRLFGEYTGMAFQIKDDLFDF
                     GDIDVGKPLGIDIKEKKMTLPLIYALQNTDRSTRRQMINIIKNHNDNKHKVAEVIRFV
                     RSSGGIEYAGQVMNDYRNRALSILAEFDDNPAKTSMQNLVMYVTERAK"
     gene            complement(3614..4933)
                     /gene="murA"
                     /locus_tag="PLE23_07660"
     CDS             complement(3614..4933)
                     /gene="murA"
                     /locus_tag="PLE23_07660"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMX83177.1"
                     /translation="MSAEAFEVEGGHRLKGVLVPQGAKNEALQVICVPLLTNEKVTIH
                     NIPDILDIRLQLELLEGLGVKLKKLAPGSIEFDASGVDIRFFESDDYLIKAKRIRGSV
                     MLMGALLNRFGRAFLPKPGGDKIGRRRLDTHFIGFEKLGATLVYDEQKDHYFLDGTNL
                     KGNYILMDEISVTGTANVIFAAVKAHGTTTIYNAACEPYVQQLCKMLNRMGARISGIG
                     SNLLIIEGVTTLGGTEHTCLPDMIEIGSFIGLAAMTQSDITIKNCNINELGNILPTFD
                     RFGIRMEIIGDDIHIPEQEKYTIRNFIDGSILTVYDSPWPGFTPDLMSILLVVATQAK
                     GSVLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGIDRAYKLKGIEMTSPDIRA
                     GVSLLIAAMSAEGKSLIHNINQIDRGYQHIDERLNAIGAHIRRVKVR"
     gene            complement(5021..5740)
                     /locus_tag="PLE23_07665"
     CDS             complement(5021..5740)
                     /locus_tag="PLE23_07665"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HMX83178.1"
                     /translation="MSTTNILKLEYNSTKDSLVLPEYGRNIHLMIQYAKTVADYEKRS
                     AYINAIIELMYQMSPLNKNVENFRDRLWNHLYEIAGYDLDVLPPSGIKPEPEAAQKAT
                     YNITYPKSDSKLRHYGHNVKKLIAKALETDDEAKKEGLVNTIGSYMKLAFRTWNREHY
                     VTDDVIKSDIITLFDGQLSLEEDKVFDNIKPANTNMQQRRYTQGQNRKRPQNGNRNNQ
                     NYNRNRPNSNTNQGNRNKWRK"
     gene            complement(5747..>7018)
                     /gene="glmS"
                     /locus_tag="PLE23_07670"
     CDS             complement(5747..>7018)
                     /gene="glmS"
                     /locus_tag="PLE23_07670"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMX83179.1"
                     /translation="YIASDATPIIEYTKKVVYLNDEEIAVVKKDGNLQIFTINNELQK
                     PKVQELDMEIERLEKAGYDSFMLKEIYEQPQTIADAMRGRMNAGESWIRLGGIAEYAN
                     KMVNAKRFIIVACGTSWHAGLIAEYLFEDLARIPVEVEYASEFRYRNPIINENDVVIA
                     LSQSGETADTLAALELAREKGALIYGIVNVVGSSISRLTDAGSYIHAGPEIGVASTKA
                     FTGQLTLLTLMALKIAHLKGNITNSYFHELLTELENIPAKIERILEKSPKIEYIAGEF
                     KNTNNALYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKHGPIALIDENMPVIVI
                     ANNKSAYEKIASNIQEVKARKGVVIAIVEEGDDFIKNMADYTIEITATEEPLSPLLSV
                     IPLQLLAYYIAIQRGCNVDQPRNLAKSVTVE"
BASE COUNT         2036 a         1381 c         1398 g         2203 t
ORIGIN      
        1 gattgctgaa ttttacgagt tccattccga aaatttcatc aaagcctgct tttttatttc
       61 cttcttccgt tggttgttcg ctcaggtcaa atttttcagc attttcgagt ctgaatgaag
      121 tctttacggg gccgtcatct gccagaaact gcgaataaac ataccagggt ttttcgatac
      181 gggcggtaag cgttagtgtg taaagattgt cagattcttt cactgcagtg gctgtccatt
      241 ttactacttt atcagactga gcagacgcaa ttgccgataa aaataccata attccaaaca
      301 aaaatattct cataccatca atttaataaa aattcaaaat tcagtactaa tcatcaataa
      361 gtccagtttg acatgttctt taacaaatat ttatcttaaa tggtttgtgg tctttaaata
      421 ttggttaaga taaatgctgg ataagctatt gcgataagca aataattatt ctacgcagat
      481 tcaagaacat ggttcaataa tgattcgaac agtattctac catcagtatt tccgagtatt
      541 tcttccgagg ctcgttcggg gtgcggcatc ataccgaaaa catttcggcc ttcattacag
      601 atgcctgcga tgttgcggct ggcaccgttc gggttatatt catctgctac ttcaccggta
      661 gggctgcaat atctaaacag tacttgatta ttcatttcga gctgaccgag gatgttttca
      721 tcagcataat acctgccttc agcatgagct attggtattt tcaacacatc acctttcgat
      781 gttagtcttg tgaatggggt ttcgttgttt tctattttca gatgaatgtt tttgcagata
      841 aatttttggc tggagttggc aagcagtaca cccggtagca ggcctgcttc gcacaatacc
      901 tgaaagccat tgcagatacc tacaaccatg cctcctttgg ctgcaaaatc tttgactgca
      961 ctcataatcg gtgaaagtcc tgccaatgct ccgcacctga ggtaatcgcc gtatgcaaac
     1021 ccaccaggca atacaataca atctgatgtg ttgaaaccag gcaggccggt gtctttgtgc
     1081 caaactttta caacttctga cttcattaca tcacccaata cgtgaatcat gtcatcatca
     1141 caattagaac ctggaaaaac tacaactccg aatttcatat actgatttta aaaattaggg
     1201 gcaaattgcc aaaaaaacac tataaatata agtaacaaat gtatcatttc tgctgaggat
     1261 gatgaaatac tgtacaaaat aagtcctccg atgaccgata ctggtagtaa aatgctcaaa
     1321 tactgttcga tgttgcggtc tttgataaaa aatgtcatac tgatgcctat aaaaagaagc
     1381 aagtacaata tctcgatgta ctttatgttt tgaatagatt gtttgaatat tatccttgaa
     1441 tatccgagca gacatagtag aatggaaagg ataataaagc cgagtttata gattccgttt
     1501 gattgtggct cgaacttaaa attgaacata aacacatcaa gctgcgcttg tcgtataaga
     1561 tgaagagaat cattccagaa aaagagaaca gtaagcagta tgacgggtac cagcatgccc
     1621 gaacacaaca ttagatattc tttgattttg ggagcacgca atatctgcaa accgagtatg
     1681 atagcgattg taaaaaatat aaacccaggg taaaaaaaga ccgacatact aagaaaaagt
     1741 ccggcattga aaataccgcc tgctacagaa tttttcttat aaaccccaaa taattctctt
     1801 acagacaaga gtaaaaaaag attggcaaac aacattgtgg tgtcgcaaag atattcagga
     1861 aacaggctga cagtaactac ccacacgaaa cccggaaata agttaatatc acgcatcatc
     1921 cttgtcttgt tgatggtggc gttaagaata ccggcttgta atactactat gataatttgg
     1981 atgacaagca accagtattt attttctggc attatgtttt cggttattct gccaagcacc
     2041 ccggtatgtt taactgccac agtacaatca tgatgtaaaa ggtagggcag tcttaacaaa
     2101 agcatgtaaa gaataaaaac cgaattggct atcaactgat tggatcgtaa tatgcttaac
     2161 acgcttctaa ttatttgacc gacatggtat taagtgaaat tttcgaagag acaaaattac
     2221 gaagtcaaat gctgaaaatg gaatctaacg catgaataat tgaaaataga tgtaggataa
     2281 gctgatttct gattgccacc ttgatatttt taaagctttt gtccatattt ttattaaacc
     2341 caaataacgc atgtggaact taaatgaaaa tgattagtat taaaaaacca taaaaaagta
     2401 ataaaagctt agcgaactat gatttttgag tttttgaaat ggattttaag taataattat
     2461 ttgcagtaaa gaggctaatg cgttatgcgg gttaaaaaat ccgctgagct aaacgaatgg
     2521 caatatgtca ggaaaagtaa aattttcgaa tagaccagat tctgttggtt tgatattata
     2581 ttgtgtcaga atagatttat aacttgtggt caaattcata ctttggcact ttctattgat
     2641 tctatacaac aacctatcaa cgctgagttg aaaatctttg aaaccaagtt cagagattca
     2701 atgaaaagcc atgtgccttt acttgacaaa attacttatt acatcgtcaa gaggaaaggt
     2761 aaacagatgc ggccgatgtt tgtatttttg tctgccagga tactaggtaa tccttcagaa
     2821 agcacttatc atgctgcttc tatggtagaa ttactccaca cagctacctt ggtacatgac
     2881 gatgtagtgg atgattcatt tgaacgacgc ggttttttct ccattaatgc actatggaag
     2941 aacaaaatag ccgtactggt tggagattat ctgttgtcga gaggcttatt attagccttg
     3001 gaaaataaag aattccaact tttgtcaata ttatctacag ccgtgaaaat gatgagcgaa
     3061 ggcgaattgc tccagataga aaaagcaaga aaccttgata taaaggaaga aatatattac
     3121 gaagtcatca gacaaaaaac tgcttcactc atagctgccg cctgtagtgc cggggctgct
     3181 tcggtcaccg aagatgagtc tgtaatagaa cgcttcaggt tgtttggcga atacactggt
     3241 atggcattcc agatcaaaga tgacttattc gattttgggg atattgacgt aggcaaacca
     3301 ctcggtatcg atattaagga aaaaaagatg acactaccgc ttatctatgc cttacagaat
     3361 actgacagaa gcactagaag gcaaatgatc aatatcatca aaaaccataa tgacaacaag
     3421 cacaaagtag ctgaagtgat tcgttttgta cggagtagtg gcggaattga atatgccggc
     3481 caagtaatga atgattaccg taaccgggca ctgagtattc ttgcagaatt cgacgataac
     3541 ccggcaaaaa cttcaatgca gaatctggta atgtatgtca cagaaagagc caaataactg
     3601 tcaggaagtg catttaccgc acctttactc gcctgatgtg tgcaccgatg gcatttagtc
     3661 tttcgtcaat atgctggtat cctctgtcta tctggttgat gttgtgtata aggcttttcc
     3721 cttcggcaga catagctgca ataagtaatg atacaccagc tctgatatca ggtgaggtca
     3781 tctctatacc tttgagctta taagccctgt cgatacctat gacagtagct ctgtgagggt
     3841 cgcacaatat tatctgcgca cccatatcta taagtttatc tacaaagaat aacctgctct
     3901 cgaacatttt ttggtggatg agaacactcc cttttgcctg cgtggctacc accagtagaa
     3961 tactcattag gtctggcgta aaacccggcc atggagagtc atacactgtc agaatactac
     4021 cgtctataaa attgcggatt gtatattttt cctgctcggg tatatggatg tcatcaccga
     4081 ttatttccat ccttatacca aaccggtcaa aagttggaag gatgttgcca agttcgttaa
     4141 tattgcaatt tttgatagta atgtccgatt gggtcattgc tgccagacct ataaaactgc
     4201 ctatttctat catatccggc aggcaggtgt gttctgtacc accgagagta gtcacacctt
     4261 ctattatcag cagattggag ccgataccag atatcctggc gcccatccgg ttgagcattt
     4321 tgcacaattg ttgtacataa ggctcacagg cagcattgta aatggtagta gtaccgtggg
     4381 cttttactgc tgcaaaaata acgttagcag taccggtaac ggatatttca tccatcagaa
     4441 tataattgcc ttttagattg gtgccatcga ggaagtaatg gtctttttgt tcatcataga
     4501 cgagtgtagc gcctaatttt tcaaaaccaa taaaatgcgt atcgagtctg cgccgtccta
     4561 tcttatcgcc acccggcttt ggcaaaaaag cacgtccaaa cctattgagc agagcaccca
     4621 tcaacatgac tgagcctctt attcttttgg ccttaatgag gtaatcgtct gattcaaaaa
     4681 agcgaatatc cactccgctg gcatcaaact cgatcgaacc gggtgccagc tttttcaatt
     4741 tgacaccaag gccttcgagc agttcaagct gaaggcggat atcaaggatg tccggtatat
     4801 tatggatagt tactttttca tttgtgagca gtggcacaca tattacctgg agtgcttcat
     4861 ttttggctcc ctgtggtaca agtacacctt taagtctgtg tccaccttct acttcaaatg
     4921 cttctgctga catattaaat ttcattttgc agatgcgaag ttacggttat tgaaccggaa
     4981 aacagaacct tgaatgaatt ttagcttcgg gtcttatcaa ttatttcctc cacttgttgc
     5041 ggtttccttg gttggtattg ctattcgggc ggtttcggtt ataattttga ttgtttcggt
     5101 tgccattttg cggtcttttc cgattttgac cttgggtgta gcgtctttgc tgcatgttgg
     5161 tgttggcagg ttttatattg tcaaagactt tatcttcctc cagacttagc tgtccgtcaa
     5221 acagggtaat gatgtccgat tttattacat catctgtgac gtaatgctca cggttccatg
     5281 ttctaaaagc aagcttcata taagaaccaa tggtatttac caatccctct ttttttgctt
     5341 catcatctgt ctcgagtgct ttggcaataa gtttcttaac attgtggcca taatgtctga
     5401 gtttagagtc agatttcggg taggtgatat tgtaggtagc tttttgagct gcttcaggtt
     5461 caggttttat tccactcgga ggcagtacat ctaagtcata accggctatt tcataaaggt
     5521 gattccacaa gcgatctctg aagttctcca catttttatt gagcggagac atctggtaca
     5581 ttagctcgat aatggcattg atataggcag atcttttttc ataatcagca acggttttgg
     5641 cgtactgtat catcaggtgg atatttctac cgtattctgg caacaccaga gagtctttgg
     5701 tagagttata ttcgagtttt aaaatattgg ttgttgacat aagtttttat tctacagtta
     5761 ctgattttgc gagattgcgg ggttgatcaa cattgcagcc tctttgtata gcaatataat
     5821 atgcgagcaa ttgaagagga atgaccgata ataacggaga aagcggttct tctgttgcag
     5881 ttatttcgat tgtgtagtct gccatatttt taataaagtc atcaccttct tctacaatag
     5941 ctataactac acctttcctg gcttttactt cctgaatgtt acttgctatt ttctcataag
     6001 cgcttttgtt attggctatc actattaccg gcatgttttc atctatcaag gctattggtc
     6061 cgtgtttcat ttcggcagca ggatagcctt cggcatggat gtaggatatt tccttgagct
     6121 tcaatgcacc ttccaatgca accgggaaat tgtacccacg tcccagataa agtgcattgt
     6181 ttgtattctt aaattcgccg gcaatgtatt ctattttcgg tgatttttcg aggattcttt
     6241 caatttttgc cggaatgttt tccaactctg tcaataattc gtgaaaataa gaattggtaa
     6301 tgttgccttt gaggtgagca atcttgagtg ccatcagtgt cagcagtgtc agttgtcctg
     6361 taaaggcctt ggtagatgca acaccaatct ccggcccggc atggatataa gatcccgcat
     6421 ctgtcagtcg ggatatagat gagccgacca cattaactat gccgtatata agggcgcctt
     6481 tttctcttgc caattcgagc gcagccagtg tatcggctgt ttctccagat tgggacaagg
     6541 caatgaccac atcattttca ttgataatcg gattgcggta tctgaattca gaagcatatt
     6601 cgacctcaac aggtattcgt gccaggtctt caaaaagata ttcggcgatg agacctgcgt
     6661 gccatgatgt accacatgct actataataa aacgtttggc attcaccatt ttattggcat
     6721 actctgcaat gcctccaagt cttatccagc tttcgcctgc attcatcctt ccgcgcatag
     6781 catctgctat agtctgtggc tgttcatata tttccttaag cataaatgaa tcataaccgg
     6841 ctttttccag tcgttcgatt tccatatcga gttcctgtac tttaggtttt tgcaattcat
     6901 tgttaatggt aaatatctgt aagttgccgt ctttttttac aactgctatc tcttcatcat
     6961 tcagatacac aactttttta gtgtattcga tgataggcgt ggcatctgat gctatgta
//
LOCUS       DAOQQY010000140         7007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4443_length_7007_cov_6.457135, whole genome shotgun sequence.
ACCESSION   DAOQQY010000140 DAOQQY010000000
VERSION     DAOQQY010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4443_length_7007_cov_6.457135"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(271..357)
                     /locus_tag="PLE23_07675"
     tRNA            complement(271..357)
                     /locus_tag="PLE23_07675"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(321..323),aa:Ser,seq:gct)
     gene            complement(465..1880)
                     /gene="dnaA"
                     /locus_tag="PLE23_07680"
     CDS             complement(465..1880)
                     /gene="dnaA"
                     /locus_tag="PLE23_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMX83180.1"
                     /translation="MVNDHNIVWENCLKTIRGNISPQSFKTWFEPIKPIRLENEALTI
                     QVPNKIFYEWLEEHYVGLLKSTIRQEIGQKAKLEYQIPIGKTQETNISARKPTHNTSN
                     TAFVAADHIKNPFVIPGIKKVKIDPQLNPDYVFDSLIEGDCNKLARSAGMAAARQPGG
                     TAFNPLVVYGSVGLGKTHLAQAIGNEIVKNFENKNVLYVVADKFTNQIIQAIKNNAID
                     ELINFYQMVDVLIVDDIQFLANKQKTQEIFFHIFNQLHQSGRQIVLTSDKPPKDLEGM
                     EERLISRFKWGLSADLQMPDFETRVAILDAKAQQMGVEIPYPVLEYLAFNVKNNIREL
                     EGVVKSLIFSASIHKRHIDKELAKEVISNHVSTVHKEITIEFIQQMVSDQLNVPLDNM
                     KGKTRMRSAVLARQLSMFLAKNHTAKSLKTIGETLGGRDHSTVIYSCKAVQDLMDTDP
                     GFRKTVEELDKKISINATVSA"
     gene            complement(2182..3108)
                     /locus_tag="PLE23_07685"
     CDS             complement(2182..3108)
                     /locus_tag="PLE23_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HMX83181.1"
                     /translation="MKRRQFIKKISIGSLLAGSGFITPEVFAQPQTFKLTILHTNDTH
                     SRIEPFPMDGGSHQGLGGVARRAALIKKIRSEEPNVLLLDSGDIFQGTPYFNMFGGEL
                     EFKAMSAMQYDAATMGNHDFDAGLEGFVKQLPLATFPFLCSNYDFNDTIVAGKTSEYK
                     IFRKGGLKIGVFGLGVELEGLVPRDNFGKTRYLDPVIKAREVAGKLKHDYKCDLVICL
                     SHLGYKYKTSKIDDVKLAGNSEDIDIILGGHTHTFLNKPEVQTNTKGKKVVISQVGFA
                     GIVLGRLDIFFERNKKDVCISCANHEINNLID"
     gene            complement(3113..3856)
                     /locus_tag="PLE23_07690"
     CDS             complement(3113..3856)
                     /locus_tag="PLE23_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMX83182.1"
                     /translation="MKNLIFIGFLLLTACTPKVWHTSGVYHANTRIYNETITDDVAML
                     MLIQPYKQLEESKMKTIVGMTQTQLIKAKPESPLTNMLADMVQASVMLKTNDKIDATF
                     INYGGVRLGALPEGEISLGKIYELMPFDNMAVILRISGAQLQTFCNHIAKSGGWPVSK
                     SIRFEIKNQQAANITIHGNGLDPDKIYRICVPDYVANGGDDCSFLTAIPQTTYQYLLR
                     DAILDGFKRVSQDGGKVESSNDGRITTSN"
     gene            complement(3872..4396)
                     /locus_tag="PLE23_07695"
     CDS             complement(3872..4396)
                     /locus_tag="PLE23_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83183.1"
                     /translation="MNFFLELLKYTLPGLIVFLTAYFVLRQYLRHQEQMKLMELKQNA
                     NAVTLPLKLQAYERLSLFCERISIPSLILRLNQPGMNTQALRYAMLISIQKEFEHNIT
                     QQMYISEQLWKIIKIASDQVVDTINLISSRIGNDSPSEVFVESYYKFSEELKFDSLEK
                     ALAAIKQEAALLLA"
     gene            complement(4511..5260)
                     /gene="rlmB"
                     /locus_tag="PLE23_07700"
     CDS             complement(4511..5260)
                     /gene="rlmB"
                     /locus_tag="PLE23_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMX83184.1"
                     /translation="MSNVNKNLLIKGRNPVLEALNESTDIERVYLLQGITGEFEKVLR
                     HLCRKKEVPLQVIPKERMSRFVSDNHQGVVAIKTSIPYYNVEDIVAHCFENGKMPLIV
                     ILDEVTDVRNLGAIARSALSFGADALVVPTIGSAPIQADSIKASAGALLKVNVCRSAS
                     LVNTVKYLQDSGMTVIASSLEAKTPLDSLDLTVPLAVIVGSEDHGIREHLLRASDQRF
                     IIPQSAEMESLNVSVAAGIMLYEVYKQRAKV"
     gene            5361..>7007
                     /locus_tag="PLE23_07705"
     CDS             5361..>7007
                     /locus_tag="PLE23_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HMX83185.1"
                     /translation="MKKLLQIIGVLSGLLFIFPVFSQTQLPLDIALRSIESEYKNLGL
                     TSDDVRDIAVSDQIRTAHNGMTNIYFIQRHAGIEVYNAILNYTISKDGKIIYTGNRFI
                     PAISSKINTNKPTLTPQKAILAAAKILGAGNIDKPRLLTQSSDHSFVFDKGELSRSDI
                     PVKLRYQLMKDGSVKLAWDLAIDYKGANDYWSMRIDALTGDLLDKNSFTVHCNHVKGK
                     YDAADNGCHPHDITFPKNLTVSEAVAKAHAALADNQYRVIPFPFENPNQSNAKLIDNP
                     ADPVASPYGWHDLNGVPGNETTITRGNNVHAFQDRNADYTSAGDEPNGGSNLIFDFPY
                     SSAWEPDTFTNSAVVQLFYAMNFMHDFTYNFGFDEFANFQQKNYTGNGLGNDHVIALS
                     QFDGNTLANVNNADFSTPADGGNGRVRMFLWDNSLSEAKNIKVTAPFNIAGAYESKKG
                     ELGANITYANPVSGAAIVVNDGALENPTFGCEPLSQGNGAEVNGKIAIIDRGLCEFGT
                     KALNAQNAGAIGCIICNFEEATISMGKGVDGGKVTIPVLSV"
BASE COUNT         1947 a         1470 c         1400 g         2190 t
ORIGIN      
        1 gaatccgttt ggagtaccaa actgattctt ttatttagca ccctaccctt ccttccaaaa
       61 aataacaatg cttcaagaac caatgacaga gtggatttcc gcatggcgga gatcccggtt
      121 tggggggtac ctgcaacatt caggaatccg tttggagtac caaactgatt cttttattta
      181 gcaccctacc cttccttcaa ggggtacctt gggtcgggtt gggtgctaaa taaaaaaggc
      241 cccgctaagc ggggccttcc tgaattgttg cggagagagc gggattcgaa cccgcggtac
      301 agttacccgt acgacagttt agcaaactgt tggtttcagc cactcaccca tctcacctgc
      361 tcttatcttt atagggaatc tttcttccct gatagttttc caaagcggac gcaaatatat
      421 aacagctttt tagaatttgc aagaaatatt tttaaataat ttcattaagc tgatacagta
      481 gcattaattg atattttttt atcgagttct tctactgttt ttctgaagcc ggggtctgta
      541 tccatcagat cttgtacggc cttgcaagaa tagataacag tactatggtc gcggccaccg
      601 agggtttctc cgattgtttt gagtgatttg gcagtgtgat ttttggcaag aaacattgac
      661 aactgccttg caagtacggc tgaacgcata cgtgttttac ctttcatatt atccaaaggt
      721 acatttagct ggtcagatac catttgctgt ataaactcta ttgtaatttc tttatgaaca
      781 gtggatacat gattagagat tacttctttt gcaagttctt tatcaatatg ccttttatgt
      841 atagatgcag aaaaaatcaa agacttgact acaccttcga gttcgcgaat gttatttttt
      901 acattaaatg ccagatactc aagtaccgga tacggaatct ccacgcccat ctgctgtgct
      961 ttggcatcca atatagctac acgtgtctca aaatccggca tttgaagatc ggccgacaag
     1021 ccccacttaa atcttgaaat gagtcgctcc tccattcctt ccagatcttt gggtggtttg
     1081 tcggatgtaa gcactatctg tcggccactt tggtgtagtt ggttgaatat atggaagaaa
     1141 atctcttgtg tcttttgttt attagctaaa aactggatat catctacaat caacacatct
     1201 accatctggt aaaaattgat gagctcatcg atggcgttat tcttgatggc ttgtataatc
     1261 tggttggtaa atttgtctgc cactacatac agcacatttt tgttctcaaa atttttgact
     1321 atttcgttgc caatagcttg tgccagatgg gttttcccta aacctacact accatatacc
     1381 actagtggat tgaatgccgt acctccgggt tgtcgggctg ccgccatgcc tgctgacctc
     1441 gcgagtttat tacaatcacc ttcaataagt gaatcgaaca catagtcggg gttcaactga
     1501 gggtctatct taactttctt gattcctggt attacaaaag gatttttgat atgatcggct
     1561 gctacaaatg cagtattgct tgtgttgtga gttggttttc ttgcagatat gttcgtctcc
     1621 tgagttttgc cgataggtat ctgatattcc agcttggctt tttgacctat ctcctgcctg
     1681 attgttgact tcaacagacc aacatagtgt tcttcaagcc attcataaaa aatcttattg
     1741 ggcacctgta tggtaagcgc ttcattctcc agtcttatag gcttgatggg ttcaaaccag
     1801 gttttgaaac tctgaggcga aatgttaccc cgaatagttt tcagacagtt ttcccatacg
     1861 atgttgtggt cgtttaccat gttttctcag atcgattgtg ccttttaggg ctttataata
     1921 atacttaact gttgttttga gacctaagcc tcggtggaat catctcagac tccgggatat
     1981 tccctctgta tatttcattt ataagactcc atcacacgtt catggggatg caaattttgg
     2041 aaaatttttc tgaataaaaa aatgtaaaat gaattaattt tcagaaaaaa aatatgctca
     2101 tacatactca tttgataatc agcaaatgtg cagtatgttt taacatttca cttgatgttc
     2161 gctacactat catcctgatc ttcaatcaat aaggttattt atctcgtggt tagcacaact
     2221 tatacataca tctttcttgt ttctttcgaa aaatatatca agtcggccaa gtacgattcc
     2281 tgcaaaaccc acttgcgata ttacaacttt cttcccctta gtattggttt gtacttctgg
     2341 tttatttaga aatgtgtgtg tgtgcccacc aagtatgatg tcaatatctt ctgagttacc
     2401 tgcaagcttt acatcgtcaa ttttgctggt tttgtatttg taaccgagat gtgataggca
     2461 gattacgagg tcgcatttat agtcatgctt gagtttgcct gctacttcac gtgctttgat
     2521 taccggatcg aggtaccgtg ttttgccaaa attatctctt ggtaccaggc cttcaagttc
     2581 aacacccaaa ccgaatacac caattttcaa acctcctttc ctgaatatct tatactcgga
     2641 tgttttgccg gcaacaattg tatcattaaa atcataattt gaacacaaga aaggaaaggt
     2701 agcaagtggc agttgtttta caaaaccttc aagtcctgca tcaaaatcat gattacccat
     2761 agtagctgca tcgtactgca ttgccgacat agccttaaac tcaagttcgc caccgaacat
     2821 gttgaaataa ggcgttccct gaaaaatgtc tccactgtca agcaacaaga cattcggctc
     2881 ttctgaacgt atttttttaa taagtgcggc acgtctggca actccgccga gaccctgatg
     2941 agaaccaccg tccatcggaa aaggttctat cctgctgtgt gtatcattgg tatgcagaat
     3001 ggtgagtttg aatgtttgtg gttgggcaaa cacctcagga gtaatgaacc cgctgccggc
     3061 cagtagagag ccaatgctga ttttttttat aaactgcctt cttttcattg tattaatttg
     3121 aagtagttat acggccatca tttgacgact ctactttacc tccgtcttgt gacactctct
     3181 tgaatccatc cagaatggca tctcttaaaa ggtattgata agtagtctgt gggattgctg
     3241 tgaggaaaga acaatcatcc ccgccattgg caacataatc aggcacacat attcggtaaa
     3301 tcttgtcagg gtcgagtcca ttgccatgta tggtaatatt ggctgcctgt tggtttttta
     3361 tctcaaaacg tatcgatttg cttaccggcc atccgcctga ttttgctatg tggttacaga
     3421 atgtttgcaa ctgagcacct gaaatgcgca aaatgacagc catattatcg aatggcatga
     3481 gctcgtatat ctttcctaag cttatttcgc cttcaggcaa tgccccaagt cttacaccac
     3541 cataattgat aaaggtagca tcaattttgt cattggtctt tagcataaca gaagcttgta
     3601 ccatgtccgc cagcatattg gtgagtggtg actccggttt tgctttgatg agttgtgtct
     3661 gtgtcatacc gacaattgtt ttcatcttgc tttcttccaa ttgtttgtag ggttgtatca
     3721 acataagcat tgcaacgtca tcggtgattg tttcgttgta tatcctggta ttggcatgat
     3781 aaactcctga ggtatgccac actttgggtg tacatgccgt cagcaacaaa aagcctatga
     3841 atatcagatt tttcattttg caatcattat tttaagcaag caaaagggcg gcctcctgct
     3901 tgatggctgc aagagctttt tcgagtgaat cgaattttag ctcttcagaa aatttgtagt
     3961 aagattcgac aaatacctct gaagggctgt cattccctat acgggatgaa ataaggttaa
     4021 tggtatctac aacttggtca cttgctatct ttataatctt ccacaattgc tcagatatat
     4081 acatttgctg agtaatgttg tgctcgaact ccttttggat gcttatcagc atagcatagc
     4141 gcaatgcctg ggtgttcatg ccgggctggt taagtcggag tatcagagaa ggaatggata
     4201 ttcgttcaca aaacaaggaa agtctttcat aagcttgcaa tttgagaggt aaagttacag
     4261 cattggcatt ctgttttaat tccattaatt tcatctgttc ctggtgtctg agatattgtc
     4321 tgagaacaaa ataagctgtc agaaaaacaa ttaggccggg aagagtatat ttcaataatt
     4381 caagaaaaaa attcatacta cattaattat tttacaaagt taaacgctta tctgccacat
     4441 atatataata gagcaaaaat aattggaaac taccacttgt gaatttttct taaagatacg
     4501 catctgtcac ctacacctta gctcgttgct tgtacacttc gtatagcata atgcccgcag
     4561 caaccgacac attcagtgac tccatttcgg ccgactgtgg tataataaac cgctgatctg
     4621 aggcgcgcaa cagatgttct ctaatgccgt ggtcttccga gccgacgata acggctagcg
     4681 gtacagtaag atcaagtgag tcaagtggtg ttttggcctc cagactcgaa gctatcaccg
     4741 tcattcctga atcctgaagg tatttgactg tattgacaag gcttgcagag cggcatacgt
     4801 ttaccttgag caaagctccg gccgaggcct tgatagaatc tgcctgtatg ggtgcactac
     4861 cgattgttgg cactaccagc gcatctgctc caaaactaag tgccgacctg gcgatggctc
     4921 ccagatttct tacatccgtg acctcatcga gtattacgat aagtggcatc ttaccgtttt
     4981 cgaaacagtg tgctacaata tcttctacat tgtaataagg tatggatgtc ttgatagcta
     5041 caactccctg atggttatca gacacgaatc gtgacatccg ttccttcgga atcacctgaa
     5101 gtggcacttc ttttttcctg caaagatggc gtagtacctt ctcaaactct cctgttatgc
     5161 cctgtagtag ataaaccctt tcgatatctg tactttcatt cagcgcttcg agcaccggat
     5221 tcctgccctt tattagcaga tttttattaa catttgacat ttatatgcgt ataattctgt
     5281 tgaaggtaaa ctattttaca ctatttttga tttccttttt aggaaaattt ttattcacat
     5341 aaaaaaaacg cttaagctaa atgaaaaaat tgctacagat tattggtgtc ttgtccggac
     5401 tcctcttcat ttttcctgta ttttctcaga cccagctacc acttgacatt gcactcagaa
     5461 gcatcgagtc tgagtacaaa aatctgggac tcacctcaga tgatgtgcgt gacattgcag
     5521 taagtgatca aatacgtaca gcgcataatg gcatgactaa tatttatttc atccagcgtc
     5581 atgcaggaat cgaagtatat aatgctatac tcaattacac aatatcaaag gacggtaaaa
     5641 taatttatac cggcaacagg tttattcctg ctatttcttc aaaaatcaat acaaacaaac
     5701 caacactcac gccccagaaa gctatacttg cggcagcaaa gattcttggt gcaggcaata
     5761 tcgacaagcc tcgactattg acacagtcct ctgaccattc ttttgttttt gacaaaggtg
     5821 agttatcaag gtctgacata ccggtcaaat taagatatca gctgatgaaa gatggttcgg
     5881 taaagttggc ctgggatctt gccatagact ataaaggggc caatgactac tggtcaatgc
     5941 gcatagatgc ccttacggga gatttacttg acaaaaacag ttttacagtt cattgcaatc
     6001 atgtcaaggg taaatatgat gccgcagaca atggatgcca cccacacgat attactttcc
     6061 ctaagaacct aacagtatct gaagcagtag ctaaggcaca tgctgcactg gcagataatc
     6121 agtacagagt tattccattc ccttttgaaa acccaaacca gtcgaatgcg aaattaattg
     6181 acaatccggc tgaccctgtg gcctctcctt acggttggca tgacctcaat ggcgtacccg
     6241 gcaacgaaac caccattacc cgtggcaata acgtgcatgc ctttcaggat agaaatgcag
     6301 attatacgag tgccggtgat gaaccaaacg gtggcagcaa tctgattttc gattttccat
     6361 actcttctgc ctgggaaccg gatacattta ccaactcggc tgtagttcag ttgttttatg
     6421 ccatgaactt tatgcacgat tttacataca attttggctt tgatgaattt gccaatttcc
     6481 agcaaaaaaa ttataccggc aatggtctgg gcaacgatca tgttatagcc ttaagccagt
     6541 ttgacggcaa cacacttgcc aatgtaaata atgctgattt ctctactccg gcagatggag
     6601 gaaatggtag agtaagaatg tttttgtggg acaatagctt atcggaagcc aaaaatatca
     6661 aagtaacagc tccatttaat atagcaggag cgtatgaaag caaaaaaggt gaattaggtg
     6721 ccaatattac ttatgcaaat ccggtatccg gtgccgccat tgtagtgaac gatggtgctc
     6781 ttgaaaatcc tacatttggt tgtgagccat tgtcccaagg taatggtgca gaagtaaatg
     6841 gtaaaatagc aatcattgac cgaggattgt gtgagtttgg cacaaaagca ctcaatgcac
     6901 aaaatgccgg agcaatagga tgtatcatct gcaattttga agaagcaaca ataagtatgg
     6961 ggaaaggtgt tgatggtggt aaagtgacta tacccgtact cagcgtt
//
LOCUS       DAOQQY010000141         7004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4450_length_7004_cov_5.011225, whole genome shotgun sequence.
ACCESSION   DAOQQY010000141 DAOQQY010000000
VERSION     DAOQQY010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7004
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4450_length_7004_cov_5.011225"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            206..739
                     /locus_tag="PLE23_07710"
     CDS             206..739
                     /locus_tag="PLE23_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX83186.1"
                     /translation="MERELILGCLKNDRQCQKSIFEYYAGRMMTLCLRYARHHMEAED
                     ILQDGFIKIFQNLDQYKFEGSFEGWMRRIFINIALKNIKKMSFKNELIGAEVVIEESE
                     APSVISSMSEQEILHAIQELPDGYKTVFNLNVIEGFSHKEISEMLNITESTSRSQLLK
                     ARRELQKKILEQTKVRI"
     gene            736..2181
                     /locus_tag="PLE23_07715"
     CDS             736..2181
                     /locus_tag="PLE23_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83187.1"
                     /translation="MKEKDFDKIIRDQMSHYESGFSEKLWNNIELELDKKTGTRALPY
                     LAAKNILLLTSLMVIFTAVGYNVYKLYDNKKTIQPQETETLQTQKDIASLENNTSTHN
                     TTTSNNDQTLASEIQASGSSGTVRETRTQYITALNPSKSTTSNNFTKGTYHSSLSSNF
                     ITQTSTKQSAGLSSASGKSDKSSSSEYAIDQSVIAREAVVRSLLTSRVMSVESGLPNA
                     GPIFGLISDPKGNCPKFHTNVPEYFLEIFGSPDFHTSYLSVKDSEMTGYLGSRKNSET
                     ALPGYTIGLRAGMNFRNGLNLKAGMSYSAVNYRFDYRKEDVEVLKYKVVPSDTIHGNG
                     TVAYVFDTIAYVEKGIRTVRHYNSLKSIELPVWIGYQKEFLRWTVGINAGASLQLVSM
                     KTGKVLSPVLPNEPVEFSDSVNGHYNLYKRTLGVGLLGSAQFGYKLTPDLNFLVEPYL
                     KYYPTIITVDEYPLRQKLLVYGINIGLRLKI"
     gene            2242..3195
                     /locus_tag="PLE23_07720"
     CDS             2242..3195
                     /locus_tag="PLE23_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83188.1"
                     /translation="MKYNIFATIIGLLLFAGCGKNSDIFIPKDDINNLKGNINNFYLS
                     VRNTVPAEVFTIHPDVENVVTTSTGLRVLIPASAFTDSTEVTVKDDIIVKITQLSKKS
                     DFIKYSQFSNTSDFVLDWFKNINFEVTTLSGKKLLLKNDSQVTVQLPTTSISPNWKLF
                     NGTYYSTRMFQVLPNEYAVIKSAVWKDGTTGTIVEGIEFKVKKTGWVCIATPVKIEKN
                     KSLHLELPASFRSFNTMTSVSYKSGNIIYLCDGSNGVPNIFSQRIHDLKNLTVLIASD
                     MQYSYFASKTEFSEYHNDSTVVLTPQKTNYTEMVNLLDKLN"
     gene            complement(3204..3674)
                     /locus_tag="PLE23_07725"
     CDS             complement(3204..3674)
                     /locus_tag="PLE23_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase
                     family protein"
                     /protein_id="HMX83189.1"
                     /translation="MKEAKALKSVSKFHNLFDLPVLTEPVIPAKERCQLRINLIEEEL
                     KELKQAIENNDLVEAADAFCDLQYVLSGAILEFGLGKKFRKLFKEVQRSNMSKACNTI
                     QEAEETVKYYKENRQTEAFIKPKADKFLVYRTEDTKVLKSVNYSEADLKNILFE"
     gene            complement(3783..5093)
                     /locus_tag="PLE23_07730"
     CDS             complement(3783..5093)
                     /locus_tag="PLE23_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83190.1"
                     /translation="MRTFLFFLTLLITTGAFAQEDPSKAYKKASRELGNYNLDQIANR
                     AQLDEAKTNIDFASSMEPTSTQAQTWITKGEIYNELCNRDLRELMTNPKHKIQYPDAA
                     KIGNEAFNKAMTLTPKKWEVKDALKGLFENAGHMNNYAAGMYAAQQYTNAYSGFSGLL
                     NTHETLKANGQKSPLENDEKTLGDTRFATAMSALNANMPEKALPYFEQLYKDQYNAPE
                     IYSALYELTSGKDMNASLQYLEAGRKKFPEDIGLLYTEINYYLKVNKTDVLIDKLKLA
                     IEKEPANVSLYSTLGNVYDNLFQKEKDATKKADYLKNAEANYTKAIEMKPDYTDAIYS
                     MGTIYFNQAAEMTKELNAMPDDFSAAGQKKYDKKKSETDEMFKKSLPYFEKVESIDPN
                     DKNTLIALKEIYARLSNLQVSNEFKKRIEILEKGGKNDSSYFKK"
     gene            complement(5163..>7004)
                     /locus_tag="PLE23_07735"
     CDS             complement(5163..>7004)
                     /locus_tag="PLE23_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HMX83191.1"
                     /translation="GRGRVVVRGCVEIETSEQGREFIIITEIPYQVNKANLVIKIAEL
                     VNEKKIEGISDIRDESDRKGMRIVVDVKRDAMANVILSKLYKFTPLQSYFGVNNIALV
                     RGRPVVLNLKNLITEFVKFRLEVIVRRTRYELRKAEERAHILEGLLIALDHLDEVITL
                     IRNSQTVEEAKDGLMSRFGLSEIQAKAILEMRLQRLTGLERDKIKNEYDELLKTIERL
                     KAILASEALQMDIIREELTEIKNKFGDKRKTEITFDDADISIEDMIEDESVVISISHL
                     GYIKRTKATEYKTQGRGGRGAMGSRTRDQDFVDQIFVASNHDYILFFTEQGKCYWLRV
                     YELPETSRTSAGRVVQNLMNIDKDDKIKAYIVVKDLQNTDFINSHYIVFATRKGVIKK
                     TILEAFSRPRANGINAITINEGDELLDAKLTTGNSEILMAIKSGRAIRFPERTVRPMG
                     RSATGVRGVSLADDNDKVVGLICVDPEDATQTILVVSENGFGKRSLLEDYRITNRGGK
                     GVKTINVTEKTGELVTIKVISDEDDLMITNRSGILIRIAASGLRVMGRATQGVKLIRI
                     EDGDSISDIAVVKHEPEEETGESDNSTLDNDVTTDTDNDSSTENADE"
BASE COUNT         2054 a         1357 c         1275 g         2318 t
ORIGIN      
        1 ataaattata ggatttataa tatacagttt tattttgcaa agggtcaatt tttttcagct
       61 ggaaacctaa tcgtatatcg caagataatt aatttaacat tactagtatt tggatatagc
      121 cctaaaataa tttatatttg tcaggtattt ttcctcaggc aacgttttaa cagttaacag
      181 aatctctgaa atgatggatt cgagtatgga gcgtgaattg attttgggat gtctaaagaa
      241 tgatagacaa tgccaaaagt caatttttga atattatgca ggtagaatga tgacactctg
      301 cctgagatat gcacgccacc acatggaggc cgaagatatt ctgcaagatg gatttataaa
      361 aatttttcag aatctcgatc agtacaaatt tgaaggttct tttgaaggct ggatgagaag
      421 aatttttatc aatatcgccc tcaaaaatat aaaaaaaatg agcttcaaaa atgaactcat
      481 cggagcagaa gtagtcattg aagaatctga ggcgccttct gtgatttcat caatgtccga
      541 acaggaaatt ctgcatgcaa tacaggaact cccggatgga tataaaacgg tttttaattt
      601 aaatgtgata gaaggctttt cgcacaagga aattagcgaa atgctcaata tcactgaaag
      661 tacatcgcgt tctcagttgt taaaagcacg acgcgaacta cagaaaaaaa ttttagagca
      721 gactaaagtt agaatatgaa agagaaggat tttgacaaaa tcatcagaga ccaaatgtca
      781 cattatgaat caggattctc tgaaaaattg tggaacaata ttgaactcga gctcgacaaa
      841 aaaaccggta caagagctct cccctatctg gcagcaaaaa acattttgct cctgacttct
      901 cttatggtta ttttcaccgc tgttggttat aatgtgtata agctttacga caacaaaaag
      961 accattcaac cacaagaaac tgagacttta caaacgcaaa aagacattgc ttccttagag
     1021 aataatacat ctactcataa cactactacc tctaataatg atcaaacttt agcgtctgaa
     1081 atccaagcct cgggttcatc aggtactgta cgagagactc gcacacagta tattacagct
     1141 ttaaatcctt ctaaatcaac aacttcaaat aattttacaa aaggcaccta tcattcttcc
     1201 ttatcttcga atttcattac tcagacaagc acaaaacaat ccgccgggct atcaagcgcc
     1261 tccggcaaat ctgacaaatc atcttcatca gaatacgcta ttgatcaatc agttattgcc
     1321 agagaagctg ttgtccgttc tcttttaact tccagagtta tgtctgtcga atcaggctta
     1381 cccaacgcag gccctatatt tggtctcatt tctgatccaa agggaaattg tccgaagttt
     1441 catacaaatg taccagagta ttttcttgaa atattcggtt caccggattt tcacactagc
     1501 tacctgagtg ttaaagatag tgaaatgacg ggttatctcg gttcaagaaa gaattcggaa
     1561 accgcacttc ccggctatac aattggtctg cgagcaggaa tgaattttag gaacggtctt
     1621 aacctgaagg caggcatgag ttattcagct gttaattaca gatttgatta taggaaagaa
     1681 gatgttgaag tactaaaata caaagtcgta ccatccgata ccatccatgg aaacggaact
     1741 gtagcttatg tgtttgacac gattgcttat gttgaaaaag gaatacgaac agtaagacac
     1801 tacaattcct taaagagtat cgaattgcca gtttggatag gttatcagaa agaatttttg
     1861 agatggacag tgggcatcaa tgcaggtgct agcctacaac tggtgagcat gaagaccgga
     1921 aaagtacttt ctcctgtttt acccaatgaa ccagttgaat tttcggattc cgtcaacgga
     1981 cattacaatt tgtacaaacg aacccttggt gtcggtcttc taggttctgc acaatttgga
     2041 tacaaattaa cgccggattt aaactttttg gtcgaaccat atttgaagta ctaccctaca
     2101 atcattactg tggacgaata tccattgaga caaaagttgt tggtatatgg tataaatatc
     2161 ggattgagat taaaaattta aattgctcag gcaacaaact gatttcttat gcactctttt
     2221 aacttagaac acaaacccta catgaaatat aatatttttg ctactatcat aggtctgcta
     2281 ctttttgccg gatgtggaaa gaattcagat atttttatcc cgaaagatga tatcaataat
     2341 ctcaaaggta atatcaataa tttttacctt tctgtaagga atactgtacc tgctgaggtt
     2401 ttcaccattc atcctgatgt cgaaaatgtc gtgactactt ccacaggttt aagggtgctt
     2461 attccggcat cggcatttac agattcaact gaagtaaccg tcaaagacga tattattgta
     2521 aaaatcacac agctttcaaa aaaatctgat ttcatcaaat attctcagtt tagcaataca
     2581 agtgattttg tccttgactg gtttaagaat attaattttg aagtaactac attatccgga
     2641 aaaaaactac tgctgaagaa tgacagtcag gtaactgtac aactgcctac aacgagtata
     2701 tcaccaaact ggaaattgtt taacggtact tattacagta caagaatgtt tcaggttttg
     2761 ccaaacgaat acgctgtaat caaatcagca gtttggaaag atggtacaac cggtactatt
     2821 gttgaaggaa tcgaattcaa ggttaaaaaa acgggctggg tatgtatcgc cacacctgta
     2881 aaaatagaaa aaaataaaag cctgcatctg gaattgccag cctccttccg ttcatttaat
     2941 acaatgactt cggtatccta caagtcagga aatatcattt acctatgtga tggaagcaat
     3001 ggcgtaccga atattttttc tcaaagaata cacgacttaa aaaaccttac ggtattgata
     3061 gcttcagata tgcaatattc ttattttgct tcgaaaactg agttctcaga ataccataat
     3121 gattctactg tagtgcttac accgcagaaa acaaattata ccgaaatggt aaatctcctg
     3181 gataagttaa actgatttta cacttattca aagagtatgt ttttaaggtc tgcttcgctg
     3241 taatttacag atttgagcac tttggtatct tcagttcggt aaacaagaaa tttatcagct
     3301 ttaggtttaa tgaaggcttc tgtctgacgg ttttctttat aatacttcac ggtttcttcg
     3361 gcctcttgta ttgtattaca agccttgctc atattggacc gctgtacttc cttgaataat
     3421 ttgcgaaact ttttgccaag tccaaactcc aatattgctc ctgacaaaac gtattgcaga
     3481 tcgcagaacg catcggcagc ttctacaagg tcgttatttt caatggcttg tttgagctct
     3541 ttaagttcct cttcgatgag atttatcctg agttgacaac gttcctttgc aggtatcacg
     3601 ggttctgtaa gtaccggcaa atcaaataaa ttgtgaaatt tggatactga cttgagtgct
     3661 ttagcttctt tcatagttgt taggttgaat actttcctac ataatatagg agcagaaaaa
     3721 ttttagaaaa aaaacagggc agaaaatatt ctctgtcctg attgattgaa cttataattt
     3781 acttatttct taaaataaga gctatcgttt ttaccacctt tttcaaggat ttcgattcgt
     3841 tttttaaatt cgttagatac ctgaagattg ctaagtcgag cataaatctc ctttagagct
     3901 atcagcgtat tcttatcatt tggatcaata gattctactt tttcaaagta agggagtgat
     3961 tttttaaaca tttcatcggt ctcagatttt ttcttgtcat atttcttttg accggctgct
     4021 gaaaagtcgt caggcattgc attgagttct ttggtcattt cggctgcctg gttgaagtaa
     4081 atagtaccca tactatagat tgcatctgta taatcaggtt tcatttctat tgccttagtg
     4141 tagttggctt cagcattttt caagtaatcc gcctttttgg ttgcatcttt ttctttctgg
     4201 aagagattgt catatacgtt gcctaaagtt gagtataatg atacattggc aggttctttt
     4261 tctattgcca gctttagttt atcaatcaat acgtcagttt tgttcacctt taggtagtaa
     4321 ttgatttcag tatataacag accgatatct tccggaaatt tctttcttcc agcttcaaga
     4381 tattgcaatg aggcattcat atcttttcct gaagtcaact catacaatgc actgtaaatc
     4441 tccggagcat tatattggtc tttgtacagc tgttcgaaat atggaagtgc tttttcaggc
     4501 atatttgcat tcagggcaga catagccgtt gcaaatcttg tatcgcccaa agttttttca
     4561 tcattttcca gcggactttt ctgaccgttg gctttgagcg tttcatgcgt attaagcaat
     4621 ccgctaaatc cggaatatgc attggtatat tgttgtgcag cgtacatacc ggctgcgtag
     4681 ttattcatgt gaccggcatt ttcaaacagt cctttgagag catcttttac ttcccatttt
     4741 ttaggtgtca gagtcatagc cttgttaaaa gcctcgtttc ctattttagc agcatccgga
     4801 tattgaattt tgtgtttggg attagtcata agctctctga ggtcgcggtt acaaagctca
     4861 ttatatattt caccttttgt aatccaagtc tgtgcctggg tacttgtggg ttccatggag
     4921 cttgcaaaat caatatttgt ttttgcttca tcaagttgag cgcggttagc aatctggtct
     4981 agattgtaat tgccaagttc tcttgaagct tttttataag cttttgaagg gtcttcctgt
     5041 gcaaatgcac ctgtagtaat cagcaatgta aggaaaaata gaaaggttct cattctttaa
     5101 tttatttagt tattgttagt aacaaattaa tgtgatactt gcattgttta caaaatgtta
     5161 aattattcat cagcattctc agtagatgaa tcgttatccg tgtctgtagt aacatcatta
     5221 tctaatgtgc tattatcaga ttcgcccgtt tcttcttcag gttcgtgttt tactaccgct
     5281 atatcagaaa tagagtcacc atcttctata cgtataagtt tgacgccctg agtggctcta
     5341 cccataaccc ttaggccgga tgcggcaata cggataagga tacctgaccg gttggtaatc
     5401 atcagatcat cttcgtcact gataaccttt atggtcacaa gctctccagt cttttcggtc
     5461 acattgattg tttttacgcc ctttcctccc cgattagtta ttctgtaatc ttcaagtaat
     5521 gatcgttttc caaaaccatt ttctgaaacc accagaatgg tttgtgtagc atcttccggg
     5581 tccacacaga taaggccaac tactttgtcg ttatcgtccg ccaaagaaac accacgtaca
     5641 ccagttgctg atctgcccat cgggcgtacg gtacgttcag ggaatctgat tgcccgacct
     5701 gactttattg ccataagtat ttcagagttg ccggttgtta gtttggcatc caacagttcg
     5761 tcaccttcgt ttatggtaat ggcattaatt ccattagcac gaggtcggga gaatgcttca
     5821 agaatggtct ttttgataac cccttttctg gtagcgaaaa cgatataatg cgaattaata
     5881 aaatcagtat tttgcaaatc tttgactacg atgtatgctt ttatcttatc atctttatca
     5941 atattcatca agttttgaac gacccttccg gcagaagttc ttgaagtttc aggtagttcg
     6001 tacacacgca accaatagca tttgccttgt tcagtaaaga acagaatata gtcatgattg
     6061 gaggctacaa atatctgatc aacgaagtct tggtctcgtg tacggctgcc cattgcacct
     6121 cgtccacctc ggccttgcgt tttgtattca gtagctttgg ttcgtttgat atagccaagg
     6181 tgtgaaatgg agatgaccac actttcgtcc tcaatcatat cttcaatact tatgtcggca
     6241 tcgtcaaatg tgatttctgt cttgcgtttg tcaccaaatt tattttttat ttctgtaagt
     6301 tcttcacgga tgatgtccat ctgtagagct tcacttgcaa gtatggcttt aagtctttca
     6361 atagttttga gtaattcgtc atattcattt ttaatcttat ccctttcaag acctgtgagg
     6421 cgctgtagcc tcatttcgag aatggccttt gcctgaatct cagataaacc gaagcgtgac
     6481 atgagaccgt ctttggcttc ttctaccgtc tgcgaattcc taatcaaagt gatgacttca
     6541 tccaaatgat caagtgctat caataatcct tccagaatat gtgcgcgttc ttcagctttt
     6601 cggagttcat atctggttcg tctgactatt acctcgagtc taaattttac gaattctgta
     6661 ataagatttt taagattcag tacgaccggt ctgcctctga ccaaagcgat gttattgaca
     6721 ccgaaataag attgtaaagg tgtaaactta taaagcttag acaatatgac attggccatg
     6781 gcatcgcgtt ttacatccac cactatgcgc attccttttc tgtccgattc gtctcttata
     6841 tcacttattc cttcgatttt cttttcattg accagctctg caattttaat tacaagatta
     6901 gctttattta cctggtaagg tatttcggta ataatgataa actctctacc ttgttcactg
     6961 gtttctattt caacacaacc tctgaccact acccttccac gtcc
//
LOCUS       DAOQQY010000142         6948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4505_length_6948_cov_5.624256, whole genome shotgun sequence.
ACCESSION   DAOQQY010000142 DAOQQY010000000
VERSION     DAOQQY010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4505_length_6948_cov_5.624256"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /gene="mutL"
                     /locus_tag="PLE23_07740"
     CDS             complement(<1..737)
                     /gene="mutL"
                     /locus_tag="PLE23_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMX83192.1"
                     /translation="MADIIQLLPDSIANQIAAGEVIQRPASVVKELMENSIDAGTTSI
                     RLIIKDSGKSLIQVIDNGKGMSAADARLCFERHATSKIRKSQDLFEIKTMGFRGEAMA
                     SIAAVAQVELKTRRKEDELGTRIVIEASEIKIEEPCQCAVGTSISVKNLFYNVPARRK
                     FLKSDIVELRHIHDEFQHIALAFPDLFFSLYHDQTEMYHLPESNLRQRIVNMLGNNYN
                     ERLVPINEETEVLRISGFIGKPDFARKT"
     gene            887..1660
                     /locus_tag="PLE23_07745"
     CDS             887..1660
                     /locus_tag="PLE23_07745"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HMX83193.1"
                     /translation="MKTKEEIVNNWLPRYTGVALDQFGEYIILVNFSGYVKAFAELNK
                     VKINGEDRSMMSATADDITIINFGMGSPNAGTIVDLLTAIKPKAILFLGKCGGLKTKK
                     NQVGDFILPIAAIRGEGTSNDYLPAEVPALPAFALQKAISTTIREHNCDYWTGTVYTT
                     NKRVWEHREDFKEYLRSLRAMAIDMETATIFIAAFKNSIPAGALLLVSDMPMVPEGVK
                     TEESDSVVTKEFADLHLKIGIESLKKLKTNDITVKHLKF"
     gene            1672..2640
                     /locus_tag="PLE23_07750"
     CDS             1672..2640
                     /locus_tag="PLE23_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HMX83194.1"
                     /translation="MATRRKFLKNISLAPASLMIPTALAATSSVNETYTQGLVISTWD
                     FGDLGNRKAWEILAGNGRALDAVEQGVKVVEDDPDTMTVGLNSYPDRDGIVTLDACIM
                     DEKGYAGSVSALERIKNPISVARKVMEDTPHVMLVGKGAQQFALSKGFKLEKQERSPR
                     ADQAWKEWLKKSEYKPIINVEMHDTIGMIAMDKKGDICGACTTSGLAFKLHGRVGDSP
                     IIGAGMYVDNEVGGAAATGVGEYVLKTLGSFLIVELMRNGMSPQQACKEAVMRITQKY
                     PYKDVQVGFIAMNKAGETGAYSIQAGFVYSECRAGTFTKNNAQSFL"
     gene            2881..3537
                     /locus_tag="PLE23_07755"
     CDS             2881..3537
                     /locus_tag="PLE23_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX83195.1"
                     /translation="MLKVIIAEDQSIFAEGLKQTLRKSSDEEIHILSLVTNGTNLEEE
                     VRHHRPDILFLDLSLPGCDSLSMLKPIHTIAPDMRIIVFTIYQDTKMVREAFRNGADG
                     YILKTCKPCEIHEGIREVKAGKVYMGSGVSATERHIAVKQNDTSNKDWFMQQYCLTNR
                     EMEILRLLSNALTNKQISEELYISDQTVGVHRKNIMRKLGVSNSAALVRMLHHNNMLV
                     "
     gene            3637..5217
                     /locus_tag="PLE23_07760"
     CDS             3637..5217
                     /locus_tag="PLE23_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HMX83196.1"
                     /translation="MNRIEELKRRRTFGIIAHPDAGKTTLTEKLLLFGGAIQIAGAVK
                     SNKIKRSATSDFMEIEKQRGISVATSVMAFEYKGRQVNILDSPGHQDFAEDTCRTLTA
                     VDSAIVVIDVAKGVETQTEKLVEVCRRRNTPIIVFINKLDREGLDGFDLLDEIETKLG
                     LKVVPMSWPIGMGGRFKGVYNLYEKKVNLFSPHGKQSEEDTIELKDINAAELGRLVGD
                     KLAAELREELHVLESVYPALDHQKYLNGDISPVFFGSAVNNFGVKELLDCFVDIAPGP
                     LPRETEERMVAPEEEKFSGFVFKIHANMDPNHRDRIAFLRICSGTFFRNTNYLHVRQA
                     KKMKFSNPTAFMAEKKSIVEEAFPGDIIGLYDSGNFKIGDTLTEGEILHYKGIPSFSP
                     EQFRYVVNKDPMKTKQLDKGIDQLMDEGVAQVFTKADNNRKIIGTVGALQFDVLQYRL
                     KHEYGASCDYEPVSLYKACWITWDDQPALTEFLSRRKADLAKDKDDRWVFLAQSAWTL
                     KMAQENHPKIQFHFTSEY"
     gene            5268..6818
                     /locus_tag="PLE23_07765"
     CDS             5268..6818
                     /locus_tag="PLE23_07765"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMX83197.1"
                     /translation="MANQEDQFKKLVSHCKEYGFIYQSSEIYDGLSAVYDYGPYGAQL
                     KNNIKEYWWKSMVQMHDNIVGIDAAIFMHPKTWKASGHVDAFNDPLVDNKDSKKRYRA
                     DVLIENYLDKIEAKINKEVEKAAARFGESFDAAMFKATNPRVLENQEKYDAIAKRLAN
                     AMTNSDMAELKQIIEDLEISCEVSGTRNWTDVRQFNLMFSTQLGNIAGEDDKLYLRPE
                     TAQGIFVNFLNVQKTTRQKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRP
                     GEEMKWYEYWKAARLRWHLALGTPAEHLRFHDHDNVAHYANAAADIQFEFPFGFKELE
                     GIHSRTNFDLSQHMELSGKKLQYFDPEMNESYIPYVVETSIGCDRMFLALMSRALTDE
                     TVPDADGKDSTRTVLRIHPALAPVKCALLPLVRNNEELTQLASSVYHKLKASFYCQYD
                     EKDAIGRRYRRQDAIGTPYCVTVDFETMDDGCVTIRDRDSLQQERVPIDKIADIISAK
                     TDMKTLLV"
BASE COUNT         2118 a         1669 c         1487 g         1674 t
ORIGIN      
        1 gttttccttg caaaatctgg tttaccgata aatccgctga tgcggagtac ttcggtctct
       61 tcattgatag gtacaaggcg ttcgttatag ttattgccca gcatattgac tatccgctgg
      121 cgtaaattcg actcaggcag atgatacatt tcggtttggt cgtggtataa gctgaagaag
      181 agatccggaa aggcaagagc gatgtgttgg aattcgtcgt gaatatgacg tagttcgaca
      241 atgtccgatt tgaggaattt tcttcgtgcg ggtacgttgt agaacagatt ttttacactg
      301 atgctggtgc ctaccgcaca ctggcacggt tcttcgatct ttatttcaga agcctctatt
      361 actattcttg tgcccagttc gtcttcttta cgccttgttt tgagctctac ctgagcaaca
      421 gctgctatcg aagccattgc ctcgcctctg aaacccattg ttttgatttc aaaaagatcc
      481 tgcgattttc gtattttaga agtggcatgt ctttcaaagc aaagccgagc atcagcagca
      541 gacattccct taccgttatc gatgacctgt atgagcgatt tgccggagtc tttgataata
      601 agccggatag aagtggtacc tgcatcaatc gagttttcca tcaattcctt cactacagat
      661 gccggccttt gtatgacctc ccccgctgct atctggttgg caatagaatc cggcaggagc
      721 tgaatgatat cagccattta gttaaaataa tttggtgcga agatacgaaa atcaatggaa
      781 ttaattaatt ttacaaaaac tacatcttgt aacaaatatt ttaataacag ccgtatatta
      841 gtatgtttga agaaaattaa agcagacaat tgaaaaataa tccgatatga aaacaaagga
      901 agagattgta aataactggt tgccgcgcta tacgggtgta gctcttgacc aatttggtga
      961 gtacatcatt ctggtcaatt ttagtggtta tgtaaaagca tttgcagagt tgaataaggt
     1021 aaaaataaat ggagaggatc gctctatgat gtctgcaacg gctgatgata ttaccatcat
     1081 caacttcggt atggggagcc ccaatgcagg taccattgtt gaccttttaa ctgccatcaa
     1141 gcctaaggct atcttgtttt taggaaaatg tggtggacta aagacaaaga agaatcaggt
     1201 aggtgacttc atcttgccta tagctgccat acgtggtgaa ggtacctcta atgactattt
     1261 gccagccgaa gtacctgccc taccggcatt cgcattacaa aaagctattt ctaccactat
     1321 ccgtgaacac aattgtgact attggacagg cacagtctat accaccaaca aaagagtgtg
     1381 ggagcacaga gaagacttca aagaatatct cagatccctt cgtgcaatgg ctatcgatat
     1441 ggaaacagcc actattttca ttgcagcttt taagaattcg attcctgccg gtgcattatt
     1501 gctggtttcc gatatgccaa tggtgcccga aggtgtcaaa accgaagaaa gtgacagtgt
     1561 ggttacaaaa gaatttgctg acctgcattt aaaaatcgga attgagtctt tgaaaaaatt
     1621 gaaaaccaat gatattacag ttaaacattt gaaattttaa tcactataaa aatggctaca
     1681 agaagaaaat ttctgaaaaa catatcactc gcaccagcct cattgatgat accgactgct
     1741 ctagctgcaa cttcttcggt gaacgagacc tacacacaag gtctagtcat ctctacatgg
     1801 gatttcggtg atctgggtaa cagaaaggcc tgggaaatat tagccggcaa tggacgtgcc
     1861 ctagatgccg tcgaacaagg cgtaaaagta gtagaggacg accccgacac tatgacagtt
     1921 ggtcttaaca gctaccccga ccgtgatggt atcgttacac tcgatgcctg tattatggac
     1981 gaaaaaggtt atgccggaag tgtgagtgca ctggaaagaa taaaaaatcc gatatctgtg
     2041 gcacgcaaag tgatggaaga cacaccacac gtcatgctgg ttggcaaagg agcccaacag
     2101 tttgcactta gcaagggatt taaactcgaa aagcaagaaa gatcaccacg ggccgaccaa
     2161 gcatggaaag aatggctaaa aaaatcggag tacaaaccga ttatcaatgt agagatgcat
     2221 gataccatcg gtatgatagc catggacaaa aaaggcgaca tctgcggggc atgcactact
     2281 agtggtcttg cattcaaact acacggacga gtaggcgaca gcccgataat aggagccggt
     2341 atgtacgttg acaatgaagt aggtggtgct gctgctaccg gtgtaggcga atacgttctc
     2401 aaaacgcttg gctcgttcct gatagtagag ctcatgcgca atggtatgag tccacagcag
     2461 gcctgcaaag aggcagtaat gcgcattacc caaaaatacc cgtacaaaga tgtgcaagta
     2521 ggcttcatcg ccatgaacaa agccggcgaa acgggcgcct acagcataca agctggtttt
     2581 gtatattccg agtgtcgtgc aggaaccttt acaaaaaata atgctcagtc atttctatag
     2641 acaaatgtag tgacgtgttt tggtcaaacc atctgttcct ttcctcctct gcagtgctaa
     2701 gcttgcattt agctgcctaa ttacttttcc tgtattatct tattatctga gttaaatgtc
     2761 atttctgaat gttggcacaa tacgctcaga taaaatattt ttataaatat ttgacattac
     2821 aaaaaaacat aatgtatatt tgtacccgaa catattataa aaaaccttaa tccctgaccg
     2881 atgttaaaag tgatcattgc tgaagatcag tctattttcg cagaaggact caagcaaacc
     2941 ctccgtaaat cttccgatga agaaatccac atcctctcac ttgttactaa cggcaccaat
     3001 ctagaagaag aagtacgcca tcaccggcca gatatcttgt ttctcgacct cagcttgccc
     3061 ggttgtgaca gtcttagcat gctcaaaccc atacatacca tagctcccga catgcgtatc
     3121 attgtcttta ctatttatca ggacaccaaa atggtgcgtg aagcattcag aaacggtgcc
     3181 gacggctaca tcctgaaaac ctgcaaacca tgcgaaatac atgaaggcat cagagaagta
     3241 aaagcaggca aggtttatat gggcagtggc gtgtcggcaa ccgagcgaca tatagctgta
     3301 aaacagaatg acacaagcaa taaggactgg tttatgcagc aatactgtct tactaaccgc
     3361 gaaatggaaa tattaagact actgtcaaat gcactaacca acaaacagat ttcagaagaa
     3421 ctatatattt ctgaccagac agtaggcgta caccgcaaaa acatcatgcg taaactcggt
     3481 gtaagcaact cagccgcttt ggttagaatg ttgcatcaca acaatatgct ggtatgacat
     3541 aaagccttac aaattagtac acctgtttat ttcatctcta agcctgttcg tctgtttcag
     3601 gatattattg ttacctttgc agcctcaaaa tgctgtatga acagaatcga agaattaaaa
     3661 agaagacgaa ctttcggtat cattgcccac ccggacgccg gtaagaccac ccttaccgaa
     3721 aaactcctcc tcttcggtgg tgccatacag atagccggtg ccgtcaaatc aaataaaatt
     3781 aaacgctctg ctacctccga cttcatggag attgaaaaac aaagaggtat ctctgtagct
     3841 acttcggtga tggcattcga atacaaaggc cgccaggtca atatcctcga ttcccccggc
     3901 caccaggatt ttgccgaaga tacctgccga acactcactg ctgtggacag tgccatcgtg
     3961 gttatcgacg tagcaaaagg cgtcgaaaca caaactgaaa aacttgtgga agtttgccgt
     4021 cggcgcaata cgcccatcat cgtattcatc aacaaactcg accgcgaagg ccttgatggc
     4081 tttgacctgc ttgacgaaat agaaaccaaa ctcggtctca aggtagtgcc tatgtcatgg
     4141 cccataggta tgggcggacg ctttaaaggt gtgtataacc tatacgaaaa gaaagtcaac
     4201 ctattctctc cgcatggcaa acaatccgaa gaggatacca tcgaactcaa ggacattaac
     4261 gctgcagaac tcggcagact cgtaggcgac aaactcgccg ccgaactacg cgaagaactg
     4321 catgtcctcg aatccgtata tccggcgtta gatcaccaga aatacctcaa tggcgacatt
     4381 tcacccgtat ttttcggcag tgccgtcaat aacttcggcg tcaaagaact actcgactgc
     4441 tttgttgaca tagcacccgg cccattacca cgcgaaaccg aagaacgtat ggtagctcct
     4501 gaagaagaaa aattctcggg attcgtgttt aagattcacg ccaatatgga tcctaaccac
     4561 cgcgaccgta tcgcattcct gcgcatctgc tcgggtacct tcttccgcaa taccaattac
     4621 cttcatgtaa ggcaagctaa aaaaatgaaa ttctcgaacc ccactgcttt catggccgaa
     4681 aaaaaatcta tcgtagaaga ggcattcccg ggtgacatca tcggccttta tgacagcggc
     4741 aacttcaaga ttggcgacac actcaccgaa ggcgaaattc tacactacaa aggcataccg
     4801 agcttctccc ccgaacaatt ccgctacgta gtcaataaag accccatgaa aaccaaacaa
     4861 ctcgacaaag gcatcgacca actcatggat gaaggcgtgg cacaggtatt taccaaagca
     4921 gacaacaacc gaaaaatcat aggcaccgtc ggcgcactgc agtttgatgt actccagtac
     4981 aggctcaaac acgaatacgg agcctcctgc gactacgaac ccgtcagcct ctataaggct
     5041 tgctggatca cctgggatga ccagccggca ctcaccgaat tcctgtctcg ccgaaaggct
     5101 gacctcgcca aggacaagga cgaccgatgg gtattcctcg cacaaagcgc ctggacactc
     5161 aagatggcac aggaaaacca cccaaagata caattccact ttacttcaga atattaacaa
     5221 cttagatacg cttatttcgt atcaacttaa taaacaaaac cccttttatg gcaaatcagg
     5281 aagatcagtt taaaaaactc gtgtcacact gcaaagaata cggcttcatc tatcagtcaa
     5341 gcgaaatata cgacggactc agtgcagtat atgactacgg accttatggc gctcagctta
     5401 agaacaacat caaagaatac tggtggaagt caatggtaca aatgcacgac aacatagtcg
     5461 gtatcgatgc agccatcttt atgcacccca agacctggaa agcaagcggc cacgtcgatg
     5521 cttttaacga tccgctcgtt gacaacaaag actcaaaaaa gcgctatcgt gccgacgtac
     5581 taatcgaaaa ctacctcgac aagatcgagg ccaagataaa caaggaggta gaaaaagccg
     5641 ccgcacgctt cggcgaatca ttcgatgcag ccatgttcaa ggccaccaat ccgcgtgtac
     5701 tcgaaaacca ggaaaaatac gacgccatag ccaaacgcct ggccaacgcc atgactaata
     5761 gcgacatggc agaactaaaa cagattatcg aagacctcga aatctcctgc gaagtgagcg
     5821 gtacgcgcaa ctggacagac gtaagacagt tcaacctcat gttctccact caactcggca
     5881 acatcgccgg cgaagatgac aaactctatc ttcgcccgga gaccgcccag ggcatcttcg
     5941 tcaatttcct caacgtacag aagactacca gacagaaaat tccattcggt attgctcaaa
     6001 taggaaaagc attccgcaac gaaatcgtcg cacgtcagtt tatattccgt atgcgagagt
     6061 tcgaacagat ggaaatgcaa ttttttgtaa ggccgggcga agagatgaaa tggtacgaat
     6121 actggaaagc agcaagactc aggtggcatc tcgcactcgg cacacctgcc gaacacctgc
     6181 gcttccacga ccatgacaat gtcgcacact acgccaacgc cgccgccgac attcagttcg
     6241 aatttccgtt tggcttcaaa gagctcgaag gcatacacag ccgtaccaac ttcgacctca
     6301 gccagcacat ggagctgtca ggcaagaaac ttcagtattt cgaccccgaa atgaacgaaa
     6361 gctatatacc ttatgtagta gagacatcca taggttgcga caggatgttc ctcgcactca
     6421 tgagtcgcgc ccttaccgac gagacagtac ccgacgccga cggcaaagac agtacacgta
     6481 ccgtcctgag gattcacccg gcgctggcac ctgtcaaatg cgccttactg ccactcgtca
     6541 ggaacaacga agaactcacc cagcttgcat ccagcgttta tcacaagctc aaagcatcgt
     6601 tttactgtca gtatgacgaa aaggatgcca ttggccgacg ttaccgaaga caagatgcta
     6661 tcggcacacc atactgcgtc acagttgact tcgaaaccat ggacgacggt tgtgtcacca
     6721 tccgtgaccg cgacagcctg cagcaagaac gcgtaccaat tgacaaaata gcagacatca
     6781 tctctgccaa gacagatatg aagaccttac tcgtctgata cttttaaatc agatggtcat
     6841 taaatattgc cggtttcaac atttgaggcc ggtattttta ttttgcagtt atgtagggcg
     6901 tgcccctgtc gggtcaggct atccgcctta cttcgtgccg gcttctat
//
LOCUS       DAOQQY010000143         6930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4519_length_6930_cov_4.919709, whole genome shotgun sequence.
ACCESSION   DAOQQY010000143 DAOQQY010000000
VERSION     DAOQQY010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_6930_cov_4.919709"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLE23_07770"
     CDS             complement(<1..411)
                     /locus_tag="PLE23_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83198.1"
                     /translation="MQKYIKIAGWLILLVPVLFSSCKTTKYLQHNEILLRKQKIKIHP
                     TEKIEGYRALSTEITNQLKQKTNRKLFGLFYTRMWLNHVLKDPPPKSGFRKWLYGTVS
                     EPPVLLDSIKAEETRIAMEGVLFNNGFFDGIVEYN"
     gene            481..1227
                     /locus_tag="PLE23_07775"
     CDS             481..1227
                     /locus_tag="PLE23_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMX83199.1"
                     /translation="MLSKHTVSILRSLHLKKYSQEHNLFLAEGEKLVEEAIKSSPKTI
                     KIICTEAWFDNNKNILIPPNISVEYATAKQISQVSTLVTPQEVLAVCQTDFTSASLRE
                     SVNKTVLYFHDLKDPGNAGTILRIADWFGYKDVFVSDESVYKYNPKLVQSSMGSVFRI
                     GYHPIHTQELMQLKGHTEIIATTMSGDSVYGSKKIEKGIIMFGSESHGLPAELIGLAD
                     KRLTIPKHKTGNAESLNLAVATGIILSYLG"
     gene            1334..1552
                     /locus_tag="PLE23_07780"
     CDS             1334..1552
                     /locus_tag="PLE23_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83200.1"
                     /translation="MKLKKNSVLVLIVGLLMSMSAMVFLNSESKQSARLALPSVEQEK
                     LEHSIPAVDPAKVVKYMFEALIKLVPQS"
     gene            complement(1554..2459)
                     /gene="fmt"
                     /locus_tag="PLE23_07785"
     CDS             complement(1554..2459)
                     /gene="fmt"
                     /locus_tag="PLE23_07785"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMX83201.1"
                     /translation="MRIVFMGTPDFAVASLDILVQNGYDIAAVITAPDRLGGRGMKQI
                     IESPVKKYAVEHNIRILQPEKLRNEDFLKELKELNAELQIVVAFRMLPEVVWNMPPLG
                     TFNLHASLLPKYRGAAPINWAIINGEKTTGCTTFFLTHEIDTGNILLQNEISIDEQDT
                     AGSLHDKLMTTGANLVLQTVRGIESQSITPFPQTGEPSLAPKIFHDTCRIDWNTSVDK
                     AYDFIRGLSPYPGAWTTVNGVQLKIYETKKHKSASVETAGKYEFLDKKVLRMYCNNGY
                     LEIISVQAEGKRRMSITEYLNGLQL"
     gene            2611..2931
                     /locus_tag="PLE23_07790"
     CDS             2611..2931
                     /locus_tag="PLE23_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HMX83202.1"
                     /translation="MTAKEFIHSLPSRVNQDAIADKETTFHFDVEGEGGGQYTVTIKD
                     GKTEVQEGLIGEAKCVVKTNNDNFMGVINGTLNPLMAIMMGKLKLSNQGEMLKYAKIF
                     GLMK"
     gene            2957..3688
                     /locus_tag="PLE23_07795"
     CDS             2957..3688
                     /locus_tag="PLE23_07795"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX83203.1"
                     /translation="MNLKLSTRTTMLYAVILYLFCACGQEGVTEDQLKSSGNTKAKLG
                     DYKAALVDYDKAIKTNPDNPRLYNNRAAALVEMGRLPEAIQDFSKSIEMDPENAMAYS
                     NRALAFATQRKFDEAVHDYSMAIKLNQHEPKLYFSRASAYSDLKQYDKAIADYNEAIK
                     LTANEPSFYSGRGIAKAQSKDLQAALQDFNKAIELKPDFGLAYTNRGLTKLVLGDKSG
                     GCEDLHRAKELGSTSAEKVLQEQCK"
     gene            3722..4288
                     /locus_tag="PLE23_07800"
     CDS             3722..4288
                     /locus_tag="PLE23_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83204.1"
                     /translation="MDSNLLLFLFFVLLFLAIVYFVTKKSRAKLLEKFKPYQNDHILL
                     QTDAYIEGFFSVLQMDVKYGDQVSENISLGPGNPVPVPYGAEITIKSQKFGALGKPFK
                     VDEKPDYQMLQVSSSKGKIFKPATWFLLFVMIGMNIIALFELIDKAYIDVIQDYTLKL
                     LLALVVIRILSNIFIKPNIIFIKKAKAV"
     gene            complement(4308..4382)
                     /locus_tag="PLE23_07805"
     tRNA            complement(4308..4382)
                     /locus_tag="PLE23_07805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4347..4349),aa:Arg,seq:cct)
     gene            4549..4917
                     /locus_tag="PLE23_07810"
     CDS             4549..4917
                     /locus_tag="PLE23_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HMX83205.1"
                     /translation="MDVESFREYCLKKAGVSEEMPFGPDNLVFKVCGKMFALNSLDTA
                     SFSVNLKCDPEYALELRDKYSGIQPGYHMNKQHWNTVNGNEDVPDKLILELTDHSYDL
                     VVSKLPAKIKTEYGLTNGKN"
     gene            4904..5947
                     /locus_tag="PLE23_07815"
     CDS             4904..5947
                     /locus_tag="PLE23_07815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX83206.1"
                     /translation="MEKIDYLIIGQGIAGTTLSYNILQRGKTCHVASDDNSISSSYIS
                     AGVINPVTGRRIVKSWMIDELMTTARLTYGGIGELLGENFITSREITKLLSDVEEENL
                     WLSKMDDLPEYLGYENDSERYRKHFNDYRSVGKIKNAMVVNIKQLLKSWEKFLTNKQL
                     LIKARISPEEMFVENEGVRYRDVNYSKLIFADGHNGLSTRFFDFLPYKNAKGEVLHIR
                     SKEYSLNEMIKGTSGIIPEGDTFWIGSNYEWDSKSELPSKANYNRILKRFDQMSKFEY
                     EIISHSAGMRACSVDRRPYIGRHPEIDAFWIMNGLGTKGMSLAPYFSLHLLNHIEENK
                     PLIKTVDIKRMLT"
     gene            complement(5978..6535)
                     /locus_tag="PLE23_07820"
     CDS             complement(5978..6535)
                     /locus_tag="PLE23_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83207.1"
                     /translation="MFPNENLYTVFKAKLIENHQYYTHRRIRTLFGLFSGAVILTVIF
                     QTAYRIPIWGILTLLAIFIIYEYFKFKNYRKTLSLIGNSTIEISPDEVIITGKDKKDV
                     EKFNLREISEICIKDKYGFPDENLTDIAREIAGKTLQNYLIFKSEKANRRFDFEFDSY
                     YMIKQFNNLIEQWKKQGKRVVVVTS"
     gene            6692..>6930
                     /locus_tag="PLE23_07825"
     CDS             6692..>6930
                     /locus_tag="PLE23_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83208.1"
                     /translation="MKKMKYFFSRIVCLFICLFGISQFLYTQNDMNIKPYYSEAFKEV
                     DKLLNQNNLPKSALEKINEIWKTASDEKNEPEQIKA"
BASE COUNT         2186 a         1258 c         1353 g         2133 t
ORIGIN      
        1 gttgtattct acaatcccgt caaaaaagcc attgttgaac aaaacgcctt ccattgctat
       61 cctggtttcc tcagctttta tagaatcaag cagcacaggt ggttcggaga cagtgccata
      121 gagccatttt ctgaatccgg atttaggagg cgggtctttg agtacatgat tgagccacat
      181 tcttgtgtag aagagtccga agagtttccg gttggttttt tgtttgagct gattagtgat
      241 ttctgtggaa agtgcgcggt aaccctcaat tttttcggtt gggtgaattt ttatcttttg
      301 ttttctgagc agaatttcgt tgtgttgcag atattttgtt gttttgcatg aagaaaacag
      361 caccggtaca aggaggataa gccaaccggc aatttttata tatttttgca acaaactcaa
      421 ttttcggtaa tctgtttctt tccggcataa agataatatt aaaatactta tagttatatc
      481 atgctttcaa aacatacggt ttctatattg cgcagtcttc atcttaagaa atattctcag
      541 gaacacaatt tattcttggc tgaaggtgaa aaactggtgg aggaagccat aaaatcatct
      601 cctaaaacca tcaaaattat ctgtacagaa gcatggtttg acaataacaa aaacatactt
      661 ataccgccaa atatttctgt agaatatgct accgccaaac aaataagcca ggtgtcaact
      721 ttggtaacac cacaggaggt tttggcagta tgccaaacag attttacatc agcgtcctta
      781 cgtgaatctg tcaataaaac tgtactgtat tttcatgacc tcaaagatcc gggcaatgca
      841 ggtaccattc ttcgcatagc agactggttt ggttataagg atgtgtttgt gtcagatgag
      901 agtgtttata aatacaaccc caaactggta caatcctcta tgggttcggt ttttcgtatt
      961 ggctaccatc ccattcatac tcaagaattg atgcagttga aagggcacac cgagatcatt
     1021 gcgactacta tgtcgggtga ttctgtttat ggttcaaaaa aaattgaaaa gggaatcatt
     1081 atgtttggca gcgaatcgca cggcttacct gcagaattga taggacttgc cgacaaaagg
     1141 ctgaccatac caaagcacaa aactggaaat gcagagtcac tcaatctggc ggtggctacc
     1201 ggtattattc tgagttatct gggttgaaaa taattttatc ttgttgtgga attatttttc
     1261 ttaaaaaatt gttaaaaagc ttatagattt gtgacttatt ggtttttgat ataaatttga
     1321 caaaactttc tttatgaaat taaagaagaa ttcggtttta gttttgatag taggcttatt
     1381 gatgagtatg tcagcaatgg tttttctcaa ttctgagtca aaacaatctg ctcggttggc
     1441 gttgccaagt gtcgaacagg aaaagttaga acatagtatt ccggcagttg atccggctaa
     1501 ggtggtcaaa tatatgtttg aggcgcttat caagcttgta ccacaatcat agcttacaat
     1561 tgtaatccgt tgagatactc ggttatactc atccttcgct ttccttcagc ttgtacagat
     1621 attatttcca gatagccatt attgcaatac atccgtagta cctttttatc taaaaattcg
     1681 tatttgccgg cagtttctac gctagctgat ttgtgttttt ttgtttcgta aatctttagc
     1741 tgtacaccat tgactgtggt ccacgctccc gggtagggcg acaatcctct aataaaatcg
     1801 taagccttat caaccgatgt gttccagtct atgcgacatg tatcgtgaaa tattttgggt
     1861 gcaaggcttg gttctcctgt ttgcggaaag ggcgtaatag attgtgattc gatgccacga
     1921 acggtctgaa gaacgagatt ggctccggtt gtcatcagtt tgtcatgcaa actgcctgct
     1981 gtgtcttgtt cgtcaatgct tatttcgttt tggaggagaa tattaccggt atcaatctca
     2041 tgtgtaagaa aaaaagttgt acaaccggtt gttttttctc cattgattat agcccagttg
     2101 ataggagcag caccacgata tttgggtaac agtgaagcat gcagattgaa tgtgcctaat
     2161 ggaggcatgt tccagactac ttccggtaac atcctgaatg caactactat ctgaagttca
     2221 gcatttaatt ctttcaactc tttgagaaaa tcttcattcc taagtttttc cggctgaaga
     2281 atcctgatgt tatgctctac tgcatatttt tttaccggcg attctatgat ttgtttcata
     2341 ccacgaccac ccaatcggtc aggagcagtg atgacggcag ctatgtcgta tccgttttgt
     2401 accaaaatat caagcgatgc cactgcaaaa tcgggagtac ccatgaaaac aattctcata
     2461 tttgtacatt tttatattaa taagctcaaa ataaataata attttgctta gcagttcatt
     2521 tgttgtatgc attattgatt tttttttcat gatgcattca aatttgaact agacaaaaac
     2581 aatttttaca taaaaaaata gaatttctac atgacagcca aagaattcat acactcacta
     2641 ccttcaagag ttaaccaaga tgccatcgcc gataaagaaa ctacattcca cttcgatgta
     2701 gaaggtgaag gaggcggaca atacactgta accatcaaag atggaaaaac agaagttcag
     2761 gaaggtttaa taggtgaagc caaatgcgtg gtaaaaacga ataatgacaa ttttatgggc
     2821 gtcatcaacg gtacacttaa tccactcatg gccataatga tgggtaagct aaaattaagc
     2881 aaccagggcg aaatgcttaa atatgcaaaa atttttggat tgatgaaata atccttaacc
     2941 tattgattct caaatcatga atctgaaatt gagtactcgt acaactatgc tttatgctgt
     3001 aatattatac ttgttttgtg cttgtggtca agaaggtgta acagaagatc aactaaaaag
     3061 ttcaggtaac acaaaagcta aactcggtga ttacaaagct gcactagtag actacgataa
     3121 ggcaattaaa accaaccctg ataacccacg cctctacaat aaccgtgctg ctgctttggt
     3181 tgagatgggt agattgccgg aggccataca agatttttca aaatcaatag aaatggatcc
     3241 tgaaaatgct atggcataca gcaaccgcgc acttgctttt gccacacaga ggaaattcga
     3301 tgaggcagtg catgattatt ctatggctat aaaactgaac caacatgaac ctaaactcta
     3361 ttttagcaga gcatcagctt atagcgacct taaacaatat gacaaagcta ttgccgacta
     3421 taatgaagct ataaaactca cagcaaacga accaagtttt tacagcggca gaggaatcgc
     3481 caaagcacaa tcaaaagatc tgcaggcagc actgcaagat tttaataaag caatagaact
     3541 aaagcctgat tttggcctag cttataccaa ccgaggcctt acgaaactgg tgttaggaga
     3601 caaatcaggc ggatgtgaag atctccatcg tgcaaaggaa ttgggcagca cttctgctga
     3661 aaaagtcttg caagaacaat gtaaatgatt aatttgattt tttttttaaa accattttgc
     3721 tatggactcc aacctgttgc tattcttgtt ttttgtattg ctatttctgg ctattgttta
     3781 ctttgtaaca aaaaagagcc gagccaaact ccttgaaaaa ttcaaacctt accagaatga
     3841 tcacattttg ctacaaactg atgcctatat agaaggtttt tttagtgttt tgcagatgga
     3901 tgtaaagtat ggagaccagg tttctgaaaa tataagccta ggtccgggta atcctgtccc
     3961 tgtaccatac ggagctgaaa ttactataaa atcacagaaa tttggtgccc tgggtaagcc
     4021 gttcaaggtc gatgaaaagc ccgattacca aatgctgcag gtaagttcgt cgaaaggcaa
     4081 aatatttaaa ccagccactt ggtttttgct atttgtaatg attggtatga atattatagc
     4141 attgtttgag ctgattgaca aggcatatat tgatgtaatc caagactaca ctttgaaatt
     4201 attattggcg ctggttgtca tcaggatatt gtcaaatatt ttcataaaac caaatatcat
     4261 ttttataaaa aaggcaaaag cagtctgaag ctctctgaag gaaaatctgg tagtcccgcc
     4321 aggaatcgaa cctggatcta gagtttagga aactcctatt ctatccgttg aactacggga
     4381 ccattggagt aagaaaattc atcaaaaacg gatgcaaaac taataatttt ttactttcca
     4441 aaatactatt tttgcaaatt cagccaaatc ctctttggaa gatgtatttt tgttgctgaa
     4501 tgaaattggt tacgaatctt ttaccatcag cattttacac acattgatat ggatgtcgag
     4561 tctttcagag aatattgtct aaaaaaagcc ggtgtaagcg aagaaatgcc tttcgggccg
     4621 gataatttgg tatttaaggt atgtgggaaa atgtttgcat taaacagcct tgatactgcg
     4681 tctttttcgg taaatctgaa atgcgatcct gaatatgcac tcgaactccg tgataaatac
     4741 tcgggtatac aaccaggcta ccacatgaac aaacaacatt ggaatactgt caacggtaac
     4801 gaggacgttc cagacaaact cattctggaa ttaaccgatc attcatacga tcttgtagtg
     4861 tcaaaattgc ctgcaaaaat caaaaccgaa tatggattaa ctaatggaaa aaattgatta
     4921 tctgataata gggcagggca ttgcgggtac tacactttcc tacaatattt tgcagagagg
     4981 caaaacctgt cacgtagcaa gtgatgacaa cagcatttca tcatcctaca tttcggcagg
     5041 tgttattaat ccggtcaccg gcaggcgtat cgtaaaatca tggatgatag acgaactgat
     5101 gacaactgca agacttacct atggtggtat aggtgaatta ttgggcgaaa atttcatcac
     5161 cagtcgggaa ataaccaaac ttctctcaga tgtggaagag gaaaatcttt ggcttagcaa
     5221 aatggatgat ctgcctgaat accttggtta cgaaaatgat tcggaacgat atagaaagca
     5281 ttttaatgac tatcgctctg ttggtaaaat aaaaaatgca atggtcgtga atatcaagca
     5341 gctactcaag agctgggaaa aattcttaac caataagcaa ttgctcatca aggcaagaat
     5401 ttcaccggaa gaaatgttcg tagaaaatga aggcgtccgg tatagagatg taaactactc
     5461 aaagctaata tttgctgatg gtcataatgg attgagtacg aggttttttg attttctgcc
     5521 ttataaaaat gccaaaggtg aagtgctgca catcagatca aaggaatatt cattgaatga
     5581 aatgattaaa ggaacatccg gcatcattcc tgaaggcgat acattctgga ttggatctaa
     5641 ctacgaatgg gacagcaaat ctgagttgcc ttcaaaggct aattacaacc gaatattgaa
     5701 acgtttcgat caaatgtcga aattcgaata cgagataatc agccattcgg caggtatgcg
     5761 tgcttgttcg gttgaccgaa gaccatatat tggccggcat ccggagattg atgccttctg
     5821 gattatgaat gggcttggta caaaaggaat gtcgctggca ccgtatttct ccctacatct
     5881 gctcaaccat atagaagaaa acaaaccact gataaaaact gtggacatca agaggatgct
     5941 gacgtgattt taaatttttg aaatctgttt ttatttctca agaggtaaca actactacac
     6001 gtttgccctg ctttttccat tgttcgatca gattgttaaa ctgtttgatc atatagtatg
     6061 aatcgaactc gaagtcgaac cttctgttag ctttttctga tttgaatatg aggtaattct
     6121 gtaaagtttt gccggctatt tctctggcaa tatcagtcaa attttcatca ggaaacccat
     6181 atttatcctt tatacatatt tcacttatct ctctgaggtt gaacttttcg acatctttct
     6241 tatcttttcc tgtgatgatt acttcatccg gagagatttc aatggtagaa ttgcctatga
     6301 gtgacaaggt ttttctataa tttttgaatt taaaatattc ataaataatg aaaattgcaa
     6361 gcagcgtaag tattccccag ataggtattc tatatgctgt ctgaaagatg actgtcagaa
     6421 tgactgcacc tgaaaacaac ccgaacaatg tacgaatcct tcggtgcgtg taatactgat
     6481 ggttttctat caattttgct ttaaacacgg tatagagatt ttcattagga aacatcaata
     6541 cacttcatgg atgactggta caaatataat gtttctcaac cgtttcatgc aattttaatg
     6601 gtcgaatatt ttgtggaaat aatagaattc ttttacaaca tccaactctg agtgcctatc
     6661 ttgcatcatt attacaaaca atctgctatt tatgaaaaag atgaagtatt tcttctcacg
     6721 tattgtgtgt ctatttattt gcttgtttgg aatttctcaa ttcttatata ctcaaaatga
     6781 tatgaacatc aaaccttatt actcggaggc ctttaaagag gtagataaac ttttgaacca
     6841 gaataattta cccaaatcag ctctggaaaa aataaatgaa atctggaaaa cggcctctga
     6901 cgaaaaaaat gaaccagaac agattaaagc
//
LOCUS       DAOQQY010000144         6926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4527_length_6926_cov_4.748217, whole genome shotgun sequence.
ACCESSION   DAOQQY010000144 DAOQQY010000000
VERSION     DAOQQY010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4527_length_6926_cov_4.748217"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..666)
                     /locus_tag="PLE23_07830"
     CDS             complement(157..666)
                     /locus_tag="PLE23_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83209.1"
                     /translation="MKALFDLITEEEKEILVDAISQITVLIAGADGKMQKEELEWAEK
                     LTRIRSFSHHKSLQTFYETVGLDFHERLHQLIDELPASTQQRTEILEAKLAHVEPILQ
                     KLPIEFGSLLYHSFTSFAKHVAKSAGGFLGIGVISTNESKLMKLSMLTPIIADVEDEE
                     EADEHTEDN"
     gene            775..3408
                     /gene="mutS"
                     /locus_tag="PLE23_07835"
     CDS             775..3408
                     /gene="mutS"
                     /locus_tag="PLE23_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMX83210.1"
                     /translation="MKNSTGDEVVPTTPLMLQYYSIKKQHPDAILLFRVGDFYETFGQ
                     DAVKTAQALGIVLTNRNNGSADTELAGFPHHSLDMYLPRLVKAGYRVAICDQLEKPSK
                     EKKIVKRGVTDVVTPGLALDDKLLDHRSNNYLASVYKLPNEHYGIGLLDVSTGEFLVS
                     EGELPYLSKLLQSFVPSEVIMPKSQLKDFTRHFGDSFYSYGIDEWVYTEDNTRELLLN
                     HFQVTGLKGFGIEEMGGAKIAAGAVLHYLHTSEKKELGHINSIARLANDKYVWLDRFT
                     IRNLELVHSQNVGGVPLINVLDQTITPMGSRMMKKWITLPLLSKIAIDSRLSQVSYFL
                     YEADIAENVEAELRQIGDLERIVSKVAMGRISPREVVQLKRALDHVKPVKEMLLNSGL
                     AELQKSGDSIHDCKAIRAQISAQLNEDAPSSISKGSVIKSGFNAELDELVNLMNNSKE
                     ILLEIQQTEAEKTGISGLKIGFNNVFGYYLEVTNKYKDQGLVPDDWIRKQTLTGAERY
                     ITNELKILETKILGAEEKITELEEKLFLELVEEIQQYIMPVQHNAQVIAKLDCILSFA
                     RVARKNRYVKPVINDSLLIDIKEGRHPVIETIIPAGESYVPNDIFLDSETQQILMITG
                     PNMAGKSAVLRMTALIVLMAQMGSFVPAESATIGIVDKVFTRVGASDNISSGESTFMI
                     EMNETANIMNNLSKRSLILLDEIGRGTSTYDGISIAWSIAEFIHNQPEARAKTLFATH
                     YHELNELENMHRRIKNFHVSTREANSKVIFLRKLQPGGSQHSFGIHVAKMAGMPREII
                     SRSNQILAELEEKHILQDDRPGNGRQTPTQNMQLSFFETKDESAEEIKKQLKQLNVNN
                     MTPVDCMLKLIELQKITEGES"
     gene            complement(3450..4007)
                     /locus_tag="PLE23_07840"
     CDS             complement(3450..4007)
                     /locus_tag="PLE23_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HMX83211.1"
                     /translation="MFDIIVVIVLGLGFYLGYQRGIIKTLFDTLSLIVAILAALKLSP
                     VVITIMQKIMSESPKMAYFLGLLLTFVLVLFLVRFVGRKVESILEAAHINFINKIGGG
                     GLQAFFFAYLLSLLIWLVNSVGLIKETTKQTAVTYPFMAAMPEVGKAAIASVKPLFKS
                     FWDKTSEIMDDVADTPELQPEAKPK"
     gene            complement(4164..4859)
                     /locus_tag="PLE23_07845"
     CDS             complement(4164..4859)
                     /locus_tag="PLE23_07845"
                     /inference="COORDINATES: protein motif:HMM:NF016658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HMX83212.1"
                     /translation="MKWLTSLLIVFHFTGFSQDASLHGIFNDLLIKHVNQAGFVDYKG
                     FKKDEKELDKYLKILEKNAPSVTASKNEQLAFWINAYNAFTIKLILKNYPVSSIELIN
                     NGKPWDLKWIKIANKTYSLNNIEHDIIRKQFKDPRVHFALNCSAKSCPPLLNQAYSAK
                     ILDAQLETQTKNFILNKKYNQINSSGVKLSMIFSWYASDFGDVRKFISKYTNIDANAK
                     TTWLEYNWQLNEQ"
     gene            5182..6405
                     /locus_tag="PLE23_07850"
     CDS             5182..6405
                     /locus_tag="PLE23_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMX83213.1"
                     /translation="MSKPSIGIIREGKIPPDARVAFTPEQCSHLMSEGWTISVQSSPH
                     RCYPDADYIKAGVPVVEDVSNADVLIGIKEVPIEQLIPNKTYFFFSHTIKKQVHNRGL
                     LQAMIEKNIAMIDYEVLTDENHERLIAFGHFAGVVGAHNGLYTYGQRTGLFHLPRMNS
                     FPHYEDAREYYSTVQWPPIKIVLTGKGRVGMGAAQVLDDMGFQSVAPSDFLSKEYSTA
                     VYTQLDVLDYVKKKSGGEFQKKEYYTHPELFEMDFMKYLAVADIFINGIFWDKKSLAF
                     FTLSDIQRTDFFTKVIADITCDIAPDSSVPTTIKASTIEDPVFGFSRKTLQECKPYTE
                     ESIDIMSIDNLPNELPRDASNSFGEKFISVILPELMNEDSAVIRRATMTKQGSLTEEF
                     SYLSDFVMHDIAQNI"
     gene            6513..>6926
                     /locus_tag="PLE23_07855"
     CDS             6513..>6926
                     /locus_tag="PLE23_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX83214.1"
                     /translation="MLTVSNLGLQYGKRVLFDEVNLTFTGGNCYGVIGANGAGKTTFL
                     KILSGQIDATRGSVSIEPGYRMAVLKQNHFEFDEYDVLSTVMMGYRELYDIMNEKNAI
                     YMDPDATEEDGMRAAELETKYGEMGGWTAESDAAEL"
BASE COUNT         2169 a         1331 c         1455 g         1971 t
ORIGIN      
        1 agcagataaa taagaatgat tctcatatcg ggttggttat tttaacaata tattgcaaag
       61 tcacattcca tgaaaaggta tgtgatttgt aaaaataaac aagtttgaag aattttctta
      121 caaaaacaat aaaaaaaaga aatcagccta tagaaattaa ttgtcctctg tgtgttcgtc
      181 ggcttcttct tcgtcttcta catcagcaat aatcggggta agcatactca acttcatcag
      241 ctttgattca ttagtagaaa taacaccaat gccaagaaag cctcctgctg atttagctac
      301 gtgtttggca aaactggtga aagaatgata aagcaatgag ccaaactcta ttggtaattt
      361 ttgaagaatt ggttctacgt gggcaagctt tgcttcaaga atttcggttc gctgttgtgt
      421 gctcgcaggt agctcatcaa tgagttggtg aagcctttca tgaaaatcaa gccctactgt
      481 ttcataaaaa gtctgaagtg atttatggtg agaaaaagaa cggatacggg ttagtttttc
      541 tgcccattca agctcttctt tttgcatttt tccgtctgca cctgctatca gtaccgtgat
      601 ttggctaatg gcgtcaacga gtatttcttt ttcttcttca gtaatcaggt caaatagtgc
      661 cttcatgtgt ttgctatatt ttaaatgaag cacaaagata accattgctg ttaaattttg
      721 taaaaattct gtattaaaag tatatttgca gttcaaatta tcaggcagca taccatgaag
      781 aactccaccg gagatgaggt cgtacctacc actcccttga tgttacaata ttattccatc
      841 aaaaaacaac atcctgatgc tattctgctg ttcagggtag gtgattttta tgaaacattc
      901 ggacaagatg ccgtaaaaac cgctcaggca ctcggtatag tacttaccaa ccgcaacaac
      961 ggttcggcag atactgagct agccggtttt ccacatcatt cactagatat gtacctgcca
     1021 aggctggtta aggctggtta tcgggtagct atttgcgatc agctcgaaaa gccatccaaa
     1081 gaaaagaaga ttgtaaaacg cggtgtaact gatgtggtga cacccggtct ggcacttgat
     1141 gataagctac ttgatcacag atctaataat tatcttgcct ctgtctataa attgccgaat
     1201 gaacattacg gtattggtct gctagatgta tcaacgggtg aatttcttgt gtcagaaggc
     1261 gaactgccat atctgagtaa attgctccaa agctttgtgc catctgaagt aatcatgcca
     1321 aaaagccagt tgaaggattt tacaagacat ttcggcgata gtttttactc ttacggcata
     1381 gatgaatggg tttatacaga agataataca cgcgaactct tgttgaatca ttttcaggta
     1441 acgggcctga agggcttcgg gattgaggag atgggtggag caaaaattgc ggccggggcc
     1501 gtactacatt atctgcacac ttcagaaaaa aaagaacttg gccatatcaa tagcattgct
     1561 cgcctcgcaa atgacaaata cgtatggtta gaccgtttta caatccggaa tcttgaactt
     1621 gtacacagcc aaaatgtcgg cggtgtacca ctcatcaacg tacttgacca gacgataaca
     1681 cctatgggca gcaggatgat gaaaaaatgg ataaccctgc cactcctctc caaaatagct
     1741 attgattcca gattgagtca ggtttcgtat ttcctgtatg aagccgacat tgccgaaaat
     1801 gtagaagccg aactaaggca gataggcgac ctcgaacgca tcgtgtctaa ggtagcaatg
     1861 ggcaggatat cgccacgtga ggttgttcag ctcaaacgag cactcgatca tgtcaaacct
     1921 gtaaaggaaa tgctgcttaa tagtggcctg gccgaattgc agaaatcggg tgacagtatc
     1981 catgactgta aggccatccg tgcacagata tcggcacagc tcaatgagga tgccccgtca
     2041 agcataagca aaggctcagt aatcaaatca ggatttaatg cagaacttga cgaactggtc
     2101 aatctgatga acaattcgaa ggagatattg cttgagatac aacaaacgga agctgaaaaa
     2161 accggtattt cgggattgaa aatagggttt aataatgtgt ttggatatta tcttgaagta
     2221 acgaacaagt acaaagacca aggactcgta cccgacgact ggataaggaa acaaacgctt
     2281 accggtgcag aaaggtacat caccaatgaa ctcaaaatcc ttgaaactaa gatattagga
     2341 gccgaagaaa aaataacgga actcgaggaa aaactgtttc tggaacttgt agaagaaata
     2401 caacaataca tcatgcccgt ccagcataat gctcaagtga ttgccaaact tgattgtatt
     2461 ctttcttttg ccagggtggc ccgaaaaaac cgatatgtaa aacctgtgat taacgattcg
     2521 ctactgattg acatcaagga aggccgtcac cctgtgatag aaaccatcat tccggcagga
     2581 gaaagctatg taccgaatga cattttcctg gactctgaga cacagcaaat attaatgata
     2641 acaggaccca atatggcagg gaagtcagca gtgttgcgta tgacagcact catcgttctg
     2701 atggcacaaa tggggtcgtt cgtgccggct gagagtgcta ccatcggcat tgttgataag
     2761 gtttttacga gggtgggcgc ttctgacaat atttcatcag gcgaatctac ctttatgata
     2821 gaaatgaatg agactgctaa tatcatgaat aacctctcaa aaagaagtct gatacttctc
     2881 gacgagatag gtcgtggtac cagtacttat gatggtattt ccatagcctg gagtattgcc
     2941 gaattcattc ataatcaacc cgaagcccgg gccaaaacat tgttcgccac acactatcac
     3001 gaactcaatg agcttgagaa catgcaccgg cgtatcaaga attttcatgt gagtaccaga
     3061 gaggccaatt cgaaagttat tttcctgaga aaactacaac ccggtggtag ccagcatagc
     3121 ttcggaatcc atgtagctaa aatggccggc atgcctcgtg aaatcataag tcgctctaac
     3181 caaatccttg ctgaattgga agaaaaacac atactgcagg atgaccgacc tggtaatggc
     3241 cgccagacgc caacacaaaa tatgcagttg tcattttttg aaactaagga tgagtcagcc
     3301 gaagaaatta agaaacaact aaaacagctg aatgtcaaca acatgacacc tgttgattgt
     3361 atgcttaagc tgatagaact ccaaaaaatt acagaaggag aaagttgaat cacttaatcc
     3421 tgtatgtaaa gaatatttga atgcccggtt tattttggct tcgcttcagg ctgaagttca
     3481 ggtgtgtcag ctacatcatc cattatctct gaggttttgt cccaaaagct tttgaataaa
     3541 ggtttaaccg aggcaatagc tgctttcccg acttcgggca ttgctgccat aaaaggatat
     3601 gtcacggcgg tttgtttggt cgtttccttt ataagaccta cagaattaac gagccatata
     3661 agcagactaa gcagatatgc aaaaaagaat gcctgcaggc ctccgccacc gattttattg
     3721 atgaaattga tatgggcagc ttcaagtatg ctttccactt ttcttcctac aaaccttaca
     3781 agaaacaata caagtacaaa tgtcagtaaa agaccaagaa agtaagccat ttttggactc
     3841 tcggacatta ttttctgcat aatggtgatg actacaggcg ataattttaa tgctgctaaa
     3901 atggcaacta tcagagataa agtatcgaac agggttttga tgattccacg ctgataacca
     3961 aggtagaagc ccaggcctag aacgataact acaataatat caaacattcg aaaagattaa
     4021 tttagatgca attataatgc ttttctaaaa ttatgtgtag ttatctgtaa gaaaatacaa
     4081 taattgtaaa gttgatgata tactttcagt atttctgaat tgtctattct caacgcattc
     4141 caccaagtgg acagtcacca gctttattgt tcattcagtt gccaattgta ctcaagccac
     4201 gttgttttag cattcgcatc aatgttggtg tatttgctta tgaacttccg tacatcacca
     4261 aaatctgagg cataccaact gaatatcata gagagtttaa ctcccgaact gttgatctga
     4321 ttgtattttt tattcagaat aaagtttttg gtctgggttt ccagttgggc atctagtatt
     4381 tttgctgaat aagcctgatt aagtaagggc ggacacgact ttgcagagca gttcaatgca
     4441 aaatgtactc tcggatcctt aaattgcttg cgtatgatgt cgtgttcaat attattcagg
     4501 ctgtaggttt tgttagctat ttttatccat ttcaaatccc atggttttcc gttgttgata
     4561 agctcgatgc tactgacagg ataattcttc agaatcaatt ttatggtaaa agcattataa
     4621 gcattaatcc agaaggcaag ttgttcattt tttgaagcag tgaccgaggg tgcatttttt
     4681 tctagtattt ttagatattt atcaagttct ttttcatcct ttttaaatcc tttataatca
     4741 acaaagccag cttggttaac atgtttaatc aacaaatcat tgaatatgcc gtgaagggaa
     4801 gcatcttgtg aaaatccggt aaagtggaac actattaata aacttgttag ccatttcatg
     4861 ctatgcgtat ttaagtgata ataagaacgc ctcagaagta taaaaagtta tgctctgcaa
     4921 atatttttac aacctattta acgaaagaaa tgactggatg ttttttcgtt cattggcctg
     4981 ggtatgagca ttgtgtgaaa taaggaggag gttaattata atgagtattt catcatattt
     5041 gtaggatgcg aattcctact tcacacagca tcaagatacc tgaatatatg cgcatgatga
     5101 aaatcatcct ggctggttta catcaagcct atctttttca ggtaaaaaac tgtagctttg
     5161 cagccctaaa aaagaactac agtgagtaaa ccatctatag gaataatcag agaagggaaa
     5221 ataccacctg atgcacgagt cgcatttaca cctgagcagt gcagtcatct catgtcggaa
     5281 ggttggacta tcagtgtcca gagcagtccg catagatgtt atcctgatgc agattatatt
     5341 aaggccggag tacctgtggt agaagatgtc agcaatgccg atgtacttat cggtataaag
     5401 gaagtgccaa ttgagcagct gatacctaat aagacatatt tcttcttttc tcataccatc
     5461 aaaaagcaag tacataatcg tgggttgtta caggcaatga tcgaaaagaa cattgccatg
     5521 atagactatg aagtactgac agacgaaaat catgaacggc tcattgcttt tggtcacttt
     5581 gccggtgtgg ttggtgctca taacgggctt tatacatacg gacaacgaac aggtttattc
     5641 catctcccaa ggatgaattc attccctcat tacgaagatg ctcgcgaata ttattcaacc
     5701 gtccaatggc cccctataaa aattgtactc acaggtaaag gaagggtagg tatgggagct
     5761 gctcaagtac tagacgatat gggatttcaa tcagtagcac catccgattt tctttcgaaa
     5821 gaatacagca cagctgttta tacgcaactc gatgtgcttg actatgtaaa gaaaaaatcc
     5881 ggaggcgaat ttcaaaaaaa ggagtactac acacatcctg agcttttcga aatggacttt
     5941 atgaaatacc ttgcggtagc cgacattttt attaacggaa tattctggga caagaaatca
     6001 cttgcctttt ttacgctttc tgacattcag cggaccgatt tctttaccaa agtcatcgct
     6061 gatattacct gtgacattgc acctgattcg tctgtgccaa caactatcaa agcaagcacg
     6121 attgaggatc ccgtattcgg ttttagcaga aagacccttc aggaatgcaa gccatatacc
     6181 gaagagagta ttgatattat gtctatcgac aacctaccga acgagttgcc acgagatgct
     6241 tccaattctt ttggtgaaaa attcatttca gtgattcttc ctgagttgat gaatgaagac
     6301 agtgctgtaa taaggcgtgc aacaatgaca aaacaaggca gtctcacaga agaattcagc
     6361 tatcttagcg attttgtgat gcacgacatt gcccagaata tttgacagtg ttctgtacta
     6421 ttacagtttg agagtagtaa ttgattttga ccaatatctg tgcctgattg gatgaaataa
     6481 ttcatacctt tgtgtaaatt taaatacagt aaatgcttac agtaagtaat ttgggcctgc
     6541 aatatggcaa aagagttctg tttgatgaag taaatctgac tttcacaggc ggcaattgtt
     6601 atggggttat cggtgccaat ggtgccggaa aaactacttt tctaaagatt ttgtcaggac
     6661 agatagatgc tacaagaggc tcggtgagta ttgagcctgg ttatagaatg gcagtactaa
     6721 agcagaatca cttcgagttt gacgaatacg atgtgctttc tactgttatg atgggctacc
     6781 gcgagctgta cgacatcatg aatgaaaaga atgccatata catggacccc gatgccaccg
     6841 aagaggatgg tatgcgggct gccgaactcg aaaccaaata tggtgaaatg ggtggatgga
     6901 cagctgaaag tgatgcagcc gaattg
//
LOCUS       DAOQQY010000145         6907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4550_length_6907_cov_5.897110, whole genome shotgun sequence.
ACCESSION   DAOQQY010000145 DAOQQY010000000
VERSION     DAOQQY010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4550_length_6907_cov_5.897110"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLE23_07860"
     CDS             <1..212
                     /locus_tag="PLE23_07860"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMX83215.1"
                     /translation="TLHDIGEDPHHTAMSKLVGLPLAIYVKNYLTGNIKGAGFKIPVT
                     ADFYTPILHELSSLGVKFRHVTRKK"
     gene            217..1524
                     /locus_tag="PLE23_07865"
     CDS             217..1524
                     /locus_tag="PLE23_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83216.1"
                     /translation="MSFRQQLTSFDSAKAFQLAGILRQAASLLTGVILAQSGFSADQI
                     GSYEYVLFFVTSFSFFWINGITQYYLSRYQDIATPNRERKVAQLLILLGMLGFGAVGA
                     THLFSWTNLSGYLTWLVGLLLLLNVPSMFAEQVLFMKKNIRHQLVFSVLVFSLHTGLL
                     LVMYWMKLRFEFIFLILFSLALLKFAFMLVITRAYIIERDTITNLTTILSASLPFIGY
                     YLATGTAGIIDGWIIKWRWNDDGMFAVYKYGARDLPLLGTLAIGLGAALLPMVNEDIK
                     IALKQIKTRSAFLMHFIFPVMIVMVAFSEWIFPYIFNNEFVETALIFSIYSFIVGSRF
                     LYPHTILSGLGYNTFLFYNSLVELGINIVLSLAFSYFFGLAGLALGSVFAYYYEKIAA
                     AVYLYKIKGIRLQEYTPMSLYLFYNFLLAFIFVISHFKIFKEL"
     gene            1521..2399
                     /locus_tag="PLE23_07870"
     CDS             1521..2399
                     /locus_tag="PLE23_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HMX83217.1"
                     /translation="MILINGAELAEEILQEIRQEVEILVANGKRRPHLAAVLVGNNPA
                     SLSYVTNKVKSCETAGFRSSLIKRDENCTEQELISLIEELNNDPEIDGFIVQLPLPRH
                     IDADNILLKIDPKKDVDGFHPLNFGRMAQGLPAFLPATPSGILQMLERYKIETDGKHC
                     VVIGRSNIVGNPMSILMARKAYPGNCTVTIVHSRTSNLKDLVKQADIIIAAIGIPKFV
                     TSDMVKEGAIVIDVGINRVADASTKSGFRLVGDVDFEHVAPKASFITPVPKGVGPMTV
                     CALMINTLKSAKSEIY"
     gene            2400..3227
                     /gene="prmA"
                     /locus_tag="PLE23_07875"
     CDS             2400..3227
                     /gene="prmA"
                     /locus_tag="PLE23_07875"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HMX83218.1"
                     /translation="MKDYKCIKIICPPDVQELMLAAVDEDDFEVYEQTDEALLCYLPE
                     EKLTDEKLKGIETLIHSFGWYYEISNIPYKNWNEAWEQNFEPIRVDDFVGVRAEFHKA
                     FDNVRFDLVINPKMAFGTGHHETTYQMMEMMQTIDFKAKSVLDYGCGTGILGILAAKL
                     SAAIIEGNDITEESIENTIENCSINKVDNFTVSLGKLDLYDGKVFDIILANINRNVLL
                     ESANALFQNMSHDGHLLTSGYMPEDQTLIEECYTEAGFRMESMTQKAYWLCHHWVKQ"
     gene            3294..>6907
                     /locus_tag="PLE23_07880"
     CDS             3294..>6907
                     /locus_tag="PLE23_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83219.1"
                     /translation="MMKYLPYLKNVLITCMLFLGLTEARAQQRTEPFAEDQKEFMKQF
                     GEFFTQSKTQVMNDLYKDFESAVKKQIITTENLSIIRATCNKFIELKLSANPYFKAYI
                     ECMLVIKNRPQHAANFNAWHDILNQIVADVQNRKFNQLEDYMSFSRGFFEMNAFRTSP
                     GSHSWLTGKDKYQMKYENKDLSLVFDDLDLTCIRKNDSIVISSTKGTLKPFEQTWKGQ
                     GGKVDWRRSDVKEIFAELDTYNLDLKKNEYTAENVLITYPSLFPSQKIKGRLTDKVVV
                     ANKATEGSYPRFESYEKILDIKDIGGGIIYKGGFRLEGTSVYGSGDKENKAQIWIKNS
                     ANKTVFTGTAETFIIRKGERVAADRVRSVIYFDEDSVYHLSLNLKFDIKQKEMTLERG
                     TKGNDRSPIFDTYHKVDISADKFKWLIDKDSIIIGEKSIAFAKSRDEVVFESDKYFDE
                     IDYRRIQSIGTTNPIANLRIATQNYGRNMDVETAAKAIDPKFSLSNIQSLLYEMLAKG
                     FVNYDPETGQVEIRDKTIHYADASQKKVDYETISILSKTLENNAEWSLKTGKINVFGV
                     DKVEFSRFQRVALKPLNDKIVLKKNRDMDFNGKLYAGYSTFTGKKFHFVYDKYQIEMD
                     SVRFFDLFIPIGEKDKNGIPVANPIESRIENTFGVLNIDAPSNKSGVQDLPAFPSFQS
                     KGLSYVFYDRLAIYDGVYKRDSFYFELDKFSFQGLDKIKGENINLKGRMFSTNIFPVF
                     NETLSLQEDQSLGYLTKTPADGFPVYVKKGVYKGNILLSNAGLQGKGSLRYIKSNFDS
                     DDILFRPKILTASAQQFNIDEGKEGNALFPKVNGQNVLVDWRPYQDSMYIRTKEKAFT
                     MFREGIHTLKGLLIMTPQGLKARGQLNWEQGLADAKVFNFGLYNASSDTMDLKIRAVN
                     SDDLAFDTKNIRGILDFENQKGSFVANSQNISTTMPYNKYSTSMNEFEWDMKNETITF
                     KADPNKPAVFRSFAEDQDSLSFRGKTAFYDLKTNLLKIGGVDVINSCDAYIYPESGDI
                     EIKKGGEMSTLNNASILADTITKFHTITKATVDIKGKKLYTAKGFYQYNIKDKEQEIF
                     FDNIIGQRVGKGKKSQKATLTTAEANLTDSLNFYLGPKMRFYGKMKLTANKKDVGFDG
                     FANIESPLFKEKNWFSIDNEVNRDDVRLYYNKSKNPTGNPVRSGFFLSKSTAQLYPRF
                     "
BASE COUNT         2269 a         1277 c         1417 g         1944 t
ORIGIN      
        1 gtacattgca tgacattggt gaagacccac accatacagc aatgtcaaaa ctggtaggtc
       61 tgccactcgc tatttatgtc aagaattacc ttacaggcaa tatcaaaggt gcaggattca
      121 agattccggt tacggcagat ttttacacac caatactaca cgaactcagt tcacttggcg
      181 taaaattcag acatgtgacc cgaaaaaagt aagtagatga gcttccggca acaactcaca
      241 agttttgatt cagccaaagc ctttcagctg gccggaatac taaggcaggc cgcatcgcta
      301 ctcacgggcg ttatccttgc ccagtcaggc ttttcggccg atcagatagg ttcgtatgag
      361 tatgttctat tctttgtcac atcattcagt tttttttgga tcaacggtat tacccagtat
      421 tatttgtcgc gttatcaaga cattgccacg cctaaccgtg agcgtaaagt cgctcaatta
      481 ctcattttac tcggaatgct tggttttgga gcagttgggg cgacacattt gttttcgtgg
      541 acgaatctca gcggctacct gacttggttg gttggtttgc tattattgtt gaatgttcca
      601 tccatgtttg cagagcaggt cctcttcatg aagaagaata taaggcatca acttgtattt
      661 tctgttttgg tttttagctt gcataccggc ttgcttttgg ttatgtattg gatgaaactt
      721 agatttgaat tcatcttttt aatcctattt agtcttgcat tgctgaaatt cgccttcatg
      781 cttgtaataa ctcgggctta tatcattgaa agagatacaa ttactaattt gacaacaata
      841 ctttctgctt ccttaccatt tatcggatat tatctggcaa caggtactgc cgggataata
      901 gacggttgga ttatcaaatg gcgttggaat gacgatggaa tgtttgcagt ttacaaatac
      961 ggagctcgcg atttgccact gcttggtaca ctggctattg gtcttggtgc ggctttgttg
     1021 cctatggtaa atgaagatat caaaattgca ttgaagcaga taaaaactcg atcggccttt
     1081 ttgatgcact ttatttttcc cgtaatgatc gtaatggtag cattttctga atggattttc
     1141 ccatacattt ttaataatga attcgtagaa actgctctaa tcttcagtat atattccttt
     1201 atcgttggat caagatttct atatccgcat acgattctgt ccggcttggg ctacaataca
     1261 ttcttatttt ataattcttt ggtagaattg ggcatcaata tagtgttgag tctggcattt
     1321 tcatattttt tcggtttagc aggtctggca ttaggttcgg tttttgcata ttattatgaa
     1381 aagattgcgg cggcggttta tttgtacaaa atcaaaggaa tcagactaca ggaatataca
     1441 cctatgtcac tttatttatt ttataatttt ttgttagcat ttatatttgt aatatcgcat
     1501 ttcaaaatat ttaaagaact atgattttaa tcaatggagc agaattggcc gaagaaattc
     1561 tacaagaaat aagacaagaa gtagaaatat tggtagcaaa cggtaaaaga agacctcact
     1621 tggcggctgt attggtcggt aataacccgg catctctttc gtatgtaacc aacaaagtaa
     1681 agtcttgtga aacagcagga ttcagatcaa gccttatcaa acgagatgaa aattgcacag
     1741 aacaagagct tatttctcta attgaggaac taaataacga cccggaaata gacgggttca
     1801 ttgtgcagct gccattgcca cgacatattg atgccgataa tatcctgctt aagattgacc
     1861 ctaaaaagga tgttgatggc tttcacccac tcaattttgg acgaatggca caaggtctgc
     1921 cagctttttt accagcgaca ccaagcggaa tactacaaat gttggaaaga tacaaaatag
     1981 aaactgatgg aaaacattgt gtagtcatcg gcagatcaaa tattgtgggc aaccccatga
     2041 gtattctgat ggccagaaag gcttacccgg gcaattgtac tgttacgatt gttcacagcc
     2101 gtacctccaa ccttaaggat ttagttaagc aggcagatat catcattgca gctattggca
     2161 ttccaaaatt tgtaacttct gatatggtta aagaaggagc cattgttata gatgtaggaa
     2221 tcaacagagt agccgatgct tcaactaaaa gtggattcag actggttgga gatgttgatt
     2281 tcgagcatgt tgcacctaaa gcatctttta ttacaccggt accaaaaggt gtagggccaa
     2341 tgacagtatg tgccctcatg ataaatacct taaaatctgc taaatctgaa atttactaga
     2401 tgaaagatta taaatgtata aaaattatct gccctccgga tgttcaggaa ctgatgttgg
     2461 cagcagttga cgaagatgat tttgaagtat atgaacagac cgatgaagcc ttgttgtgct
     2521 atttgccaga agaaaaactg acagatgaaa aattaaaagg aatagagaca ctgatacatt
     2581 cttttgggtg gtattatgaa ataagcaata tcccatacaa aaactggaat gaggcctggg
     2641 agcaaaattt tgaaccgata cgggttgacg actttgtagg cgtcagggct gaatttcaca
     2701 aagcatttga taatgtcaga ttcgaccttg tcatcaatcc gaaaatggca tttggtaccg
     2761 gacaccatga gaccacctac caaatgatgg aaatgatgca aacgattgac ttcaaggcaa
     2821 aatctgtact tgactatgga tgtggcactg gcatactagg aatacttgcc gcaaagctga
     2881 gcgcagctat tattgaaggt aatgatataa cggaagaatc tatagaaaat accattgaaa
     2941 actgttccat taacaaggtt gataatttta ctgtttcact tggcaagctt gatctctatg
     3001 atggaaaagt atttgatatc atcctggcaa atataaacag aaacgtgctg ttagaatcag
     3061 ctaatgcact gtttcaaaat atgagtcatg atggacatct gctgacttca gggtacatgc
     3121 ctgaagacca gactttgata gaagagtgtt acactgaggc tggatttcgt atggagtcaa
     3181 tgacacagaa ggcttactgg ctgtgtcatc actgggtaaa acaataattt attgtatgta
     3241 ttggttataa gcttagtaaa taatttattc tgagattatc ttattttaat aatatgatga
     3301 aatacttacc atatttaaag aatgtactaa taacatgtat gttgttttta ggtcttaccg
     3361 aagcccgggc acagcaacgt acggaacctt tcgccgaaga ccagaaggaa ttcatgaagc
     3421 agttcggaga gtttttcaca cagtcaaaga cacaggtgat gaacgatctt tacaaagatt
     3481 ttgaatctgc agtaaaaaag cagattatca caacagaaaa tctaagtata attagagcaa
     3541 cctgcaataa attcattgag cttaaactat ctgccaaccc ttacttcaaa gcctatatcg
     3601 aatgtatgct tgtgattaag aacaggccac agcacgctgc aaactttaat gcatggcacg
     3661 acatactcaa tcagatagtt gccgacgtac aaaaccggaa atttaatcaa cttgaagatt
     3721 atatgtcgtt ttcgcgtgga ttttttgaaa tgaatgcctt cagaacatcg ccaggtagtc
     3781 actcatggct tacaggcaag gataagtatc agatgaaata tgaaaacaaa gatctcagct
     3841 tggtatttga tgatcttgac ctcacctgca tacgcaaaaa cgattcaata gttataagca
     3901 gtacaaaagg cactctcaaa ccattcgaac agacctggaa agggcaaggc ggaaaagttg
     3961 attggcgaag aagcgatgtg aaagagattt ttgcagagct tgatacttac aatcttgatc
     4021 taaaaaagaa cgaatataca gctgaaaatg tgttgattac ctatccttca ctttttccga
     4081 gtcaaaaaat caaaggcaga ttgacagaca aggtagtggt agcaaacaaa gcaaccgaag
     4141 gatcgtaccc acgcttcgaa tcctacgaaa aaatactgga cataaaagac attggtggcg
     4201 gaattattta taagggtggt ttcaggcttg aaggtacctc tgtgtatggc tccggagata
     4261 aagaaaacaa agcacagatt tggataaaaa attctgccaa caaaaccgtt tttaccggca
     4321 cagcagaaac attcattata agaaaagggg agcgagtggc ggcagacagg gtcaggtcgg
     4381 ttatctattt tgacgaagat tctgtgtacc atctatcgtt aaatttaaaa tttgacatca
     4441 aacaaaaaga aatgacgctc gaacgtggta ccaagggaaa tgaccgtagt ccaatctttg
     4501 acacatacca caaagtggac atttctgccg ataaattcaa atggctcata gacaaagaca
     4561 gtattatcat tggtgaaaag tctatagcat ttgccaaatc aagagatgaa gttgtttttg
     4621 aatctgataa atattttgat gaaattgatt acagaagaat acaaagtatt ggtacgacca
     4681 atcctatagc caacctaagg attgctacac agaattatgg ccgcaatatg gatgttgaaa
     4741 cagcagcaaa ggccatcgat ccaaaattca gcttgagtaa tattcaatca ttattgtacg
     4801 aaatgctggc aaaaggattt gtcaattatg atccggaaac cggacaagta gaaatacgcg
     4861 acaaaaccat acattatgcc gatgcaagcc aaaagaaagt agattatgaa accataagca
     4921 tcttgtcaaa aacactcgag aataacgcag aatggagcct aaaaacgggc aagattaatg
     4981 tttttggagt agataaagtt gaattttcaa gattccaaag ggtcgcactc aaaccactaa
     5041 atgacaaaat tgtactcaaa aaaaaccgcg atatggattt caacggaaaa ttgtacgctg
     5101 gttatagtac ctttaccggt aagaaatttc atttcgtcta tgataaatac cagatagaaa
     5161 tggactctgt caggtttttt gacctattta taccgatagg tgaaaaagac aagaacggta
     5221 ttccggtggc caacccaatc gaatcaagga tagaaaatac attcggtgta cttaacattg
     5281 atgcgccttc caacaaatca ggtgttcagg atttgcctgc atttccgagc tttcagtcga
     5341 aaggtctttc gtatgtattt tatgaccggc ttgccattta tgatggtgta tataagcgcg
     5401 attcgtttta cttcgaattg gataaattca gttttcaggg tcttgacaaa attaaaggcg
     5461 aaaacatcaa tctgaaagga agaatgtttt ctaccaacat ttttccggtc tttaatgaaa
     5521 cgctctcact acaagaagac caaagccttg gatatctgac aaaaacgcct gcggatggtt
     5581 tccctgtata tgttaaaaag ggtgtttaca aaggtaatat acttcttagt aatgccggct
     5641 tgcagggaaa aggcagtctt cggtacataa agagtaattt cgactctgat gatattcttt
     5701 tccgccccaa gatacttaca gcaagcgcac agcaattcaa tatagatgaa ggaaaggagg
     5761 ggaatgcact gttcccgaaa gtaaatggcc agaacgttct ggttgactgg agaccatacc
     5821 aggacagtat gtacatccga accaaagaaa aggcttttac aatgtttaga gaaggtatcc
     5881 atacgcttaa gggtctgctc ataatgacac cacaagggtt gaaggcaaga ggacaactca
     5941 actgggaaca agggcttgca gatgccaaag tgtttaattt tggcttgtac aatgcttcat
     6001 ctgacaccat ggacctaaag atccgggctg taaattctga tgacctcgca tttgacacaa
     6061 aaaatatccg aggtattctt gattttgaaa accagaaagg aagttttgtg gctaattcgc
     6121 agaatatcag tacaaccatg ccttacaaca aatattccac atccatgaac gagtttgaat
     6181 gggatatgaa gaacgaaacc attactttca aagccgaccc caacaaaccg gcagtgttcc
     6241 gttcattcgc agaagaccag gatagcctga gttttagagg taagaccgct ttttatgatc
     6301 tgaagaccaa cttactcaag ataggtggtg tagatgtcat taactcgtgc gacgcataca
     6361 tttatccgga atcgggcgat atcgaaatca aaaaaggtgg tgaaatgtca acacttaaca
     6421 acgcaagtat tttagcagat actattacaa aatttcacac cataacgaag gctactgtag
     6481 atattaaagg aaagaaactc tacactgcca aaggtttcta ccaatataat atcaaagaca
     6541 aagaacaaga aatattcttc gataacatca tcggccaacg tgtcggaaaa ggcaagaaat
     6601 cgcaaaaggc cacactgacc acggccgaag ccaatcttac agattcactc aatttttacc
     6661 ttggcccgaa aatgagattt tacggcaaaa tgaaactcac agccaacaaa aaagatgtag
     6721 gctttgacgg atttgctaat atcgagtcac ctttattcaa ggaaaagaat tggttttcga
     6781 tagataatga ggtcaacaga gatgatgtac gcctatatta caacaaaagt aagaatccta
     6841 ccggcaaccc tgtgcgaagt gggtttttcc tgtcaaaaag taccgcacag ctttatccaa
     6901 gattcat
//
LOCUS       DAOQQY010000146         6899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4562_length_6899_cov_7.661894, whole genome shotgun sequence.
ACCESSION   DAOQQY010000146 DAOQQY010000000
VERSION     DAOQQY010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4562_length_6899_cov_7.661894"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="PLE23_07885"
     CDS             <1..887
                     /locus_tag="PLE23_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMX83220.1"
                     /translation="MLMMFFSSTYVAVRYLLHYQEMNEMLGRGTPIPVPMSDHVRYAQ
                     LQAYIILSGVYWLLKDRSLHKMIKIIGWCSIVMMFFYIHLLAVRSGILVVYCGLLVFL
                     VLNLKKISLPVLLVIVASFVATGFFSVQYIPSLAQKLSYMKWEYRQLQEGKTIESSDS
                     GRWSSYDIGRKLYRSSPAFGIGPGDMDDLVKKMYAKYYPTEKNAKQPHNQFLHTLTGS
                     GYIGLGIFLALWVAVLWFGFHYHNKILITMVFATFAAFMVESPLETARGTAIIAFWLS
                     FWMAEGIPPDKLFRIQSA"
     gene            complement(890..1558)
                     /locus_tag="PLE23_07890"
     CDS             complement(890..1558)
                     /locus_tag="PLE23_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83221.1"
                     /translation="MRNIILTLLTFISLSFTATAQYSEAERPMSMGMKNSLSLSVNDL
                     KTSKVEDIWQDYVKQYDGKTKKDKKMKEYVTDAAKVYYIPGLSTINIYARFEERGKTT
                     TEAIIFFLDGDRFLSTSYNADQMKAANEFVRQFDIAIQKYKTNQLLEAEQKKLKGLES
                     DLKKLVRDNESLHKDIEGYKDKITKAETAISKNNGDQEKTASMITEQNKVIEKVKTDL
                     NALK"
     gene            complement(1641..2576)
                     /locus_tag="PLE23_07895"
     CDS             complement(1641..2576)
                     /locus_tag="PLE23_07895"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HMX83222.1"
                     /translation="MKYDIIINPMGGAGQSVKAWASLQREFDNSNIGYQEYFTKGTGD
                     AIDIAKRLTCKGSKRIIAVGGDGTANEVANGILQAQDEGFGTETAMTLFSSGTGNDWV
                     REHRISRRAVEFVKSLQDDRQHIQDVGLIDYYDENYKQQSRYFINVAGLAYDAFVLKA
                     TEKYKKTGLASKLYLLHVFKNLLKYKPEKVRIRADDKDFTKTVYTINIGQCRYSGGNM
                     QLVPHAVYDSGKLAVTMADSMPKWEVVLLTPKFYNGSLLNHPNVTGFECDRIEIRSLN
                     NKEVMIEADGEFLGYTPVNIRNCSKKLKIFAVESV"
     gene            2697..3596
                     /locus_tag="PLE23_07900"
     CDS             2697..3596
                     /locus_tag="PLE23_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX83223.1"
                     /translation="MDILNIRNVVKSYESKVAVDDVSLNIPQGIIFGMLGPNGAGKTS
                     LIRIITTITRPDSGKILFDNDPLIEKHTEQMGYMPEERGLYKKMKVGEQLLYLARLKG
                     LSKQEAESTIAHWFDKFDIKDWYNKKVMDLSKGMQQKVQFIATVMHRPKLIILDEPFS
                     GLDPINAKLIENEIIRMRDEGTSIIFSTHRMEQVEEICDKIVLINQGKNVLEGDVNEI
                     KNTYKENLYNIRYEGNINVHFEQFGEITHKNDNSITIKCDSSQKANDLLKWMIQNGAL
                     INSFKEILPTINDIFIRKVEGNI"
     gene            3616..4935
                     /locus_tag="PLE23_07905"
     CDS             3616..4935
                     /locus_tag="PLE23_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX83224.1"
                     /translation="MDKLWLIIQREFLTRVKSKSFLLSTFLTPLAFLVFFVIAGFIMS
                     YKEGEDKKVVVSDPANLLDKVIKDESNIYYTFSDESIDNLKKQVEQGNYSGILEIPKP
                     DSAAIRQLKVYYYSENQLGLDDQAALENQLEDRIGEYKLKAQGLDKSILENLKTNISI
                     DPEPINASQKNESSSAAAIGAALGTIMGIIMYMSVFIYGSMVMRSVMEEKMSRIVEVM
                     ISTVKPFQLMMGKIIGVGAVGLTQFILWMILIPLISMFVTMVFGFDPQQMQSQAMTGA
                     QSVDPDDLQMMVSKIFIEVNNMNWWLIIPSFIFYFLCGYILYSSLFAAVGSAVGDDLG
                     ESQSLTLPITIPVLLAFYIMFAAVRAPQSSMAVFGSIFPLFSPIVMPSRIVFDVPAWE
                     IAVSMLLLLVTTIFMVWLAGRIYRVGILMYGKKASFKELRKWMFYKD"
     gene            complement(4948..5547)
                     /locus_tag="PLE23_07910"
     CDS             complement(4948..5547)
                     /locus_tag="PLE23_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase hexapeptide repeat family protein"
                     /protein_id="HMX83225.1"
                     /translation="MFYAFKGFVPVVHETAFVHPQACVTGNVVIGRHVYVGPGAAIRG
                     DWGGIVIEDGCNVQENCTIHMFPGVTVYLCEAAHIGHGAIVHGASIGRNTLVGMNAVI
                     MDNAQVGNECIIGALTFVPAEMIIPDRSLVVGSPAKIIKQVSDDMIDWKSAGTALYQR
                     LPEECYGQLIPCEPLREMPADRPEQETLYKTWKEINSRS"
     gene            complement(5557..6390)
                     /locus_tag="PLE23_07915"
     CDS             complement(5557..6390)
                     /locus_tag="PLE23_07915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMX83226.1"
                     /translation="MSIFQNSSGGGSRIGGTLILGLIMALFTLFQYYSKTEVNPITKE
                     KQHIGISPEQEIAMGLQSAPAMAQEFGGLYPDERAQQLVKKVGQNVVRNSAAKKTPYQ
                     YDFHLLADPNVINAFALPGGQIFITYALFSQLETEDQLAGVLGHEIGHVVARHSAQRI
                     ADQQLTDGLTGAAVVATGSQSTAQMAQMIGNMINMKYGRGQELQSDDLGVRFMLEAGY
                     EPEGLLGVMKILEQSSGGQRVPEFQSTHPSPENRMGRIKEAMQKYRGLKNTKGDSDIS
                     Q"
     gene            6676..>6899
                     /locus_tag="PLE23_07920"
     CDS             6676..>6899
                     /locus_tag="PLE23_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83227.1"
                     /translation="MKINISNKNMPTVMFIAFLSMLSNSLFAQQDVVENYTAVDTIAV
                     FDPETNKEVVSYVKTELQVHSRPDEMPFMS"
BASE COUNT         1968 a         1492 c         1361 g         2078 t
ORIGIN      
        1 ccatgctcat gatgtttttt tcttcgacct acgtggcggt tcggtattta cttcattatc
       61 aagagatgaa cgaaatgctg ggacgaggta cgcccatacc ggtgccgatg tctgaccatg
      121 tacgatacgc ccagcttcag gcgtatatta ttttatccgg tgtttactgg ctgctgaaag
      181 accgttctct tcacaaaatg ataaaaataa ttggttggtg ttctattgtc atgatgtttt
      241 tctacatcca tttgttggca gtacggtcgg gtattctggt agtgtattgc gggttgctgg
      301 tttttcttgt gttaaattta aagaaaatca gcttacctgt attgttggtt atagtagctt
      361 cctttgtagc cactggcttt ttttccgtac aatacatacc aagcctggct caaaaactca
      421 gttatatgaa atgggaatac agacaactgc aagaagggaa aaccatcgaa agctcagaca
      481 gtggcaggtg gagttcgtat gatataggac gaaaactata ccgcagttca ccggcattcg
      541 gtatcggccc gggcgatatg gatgaccttg tgaaaaagat gtacgcaaag tactacccca
      601 cagaaaaaaa cgccaaacag ccgcacaatc agtttttgca cacactcaca ggaagtggct
      661 atatcggact tggtattttt ctggcattgt gggttgccgt cctatggttt gggtttcatt
      721 accataacaa gatactgata actatggtgt ttgccacctt tgctgctttc atggtcgaaa
      781 gtccgctaga gacagcacga ggcacagcca tcattgcctt ctggctatct ttttggatgg
      841 cagaaggcat tcctccggac aaattattca gaatacagag cgcttgagct tattttagtg
      901 cgttcaagtc tgtctttacc ttttctatca ccttgttttg ttcggtaatc atactggctg
      961 ttttctcctg gtctccatta ttttttgaga tggcagtttc ggctttggtt attttgtctt
     1021 tatagccttc aatatcctta tgtaaggact cattatctct taccagtttt ttaagatcag
     1081 attccagacc ttttagtttt ttttgttcgg cttcgagcag ttggttggtt ttgtactttt
     1141 gtatggctat atcaaactgc cttacgaact cattggccgc cttcatctga tcggcattgt
     1201 agcttgtgct aagaaaacgg tcgccgtcta agaaaaagat gattgcctct gttgtggttt
     1261 tccctctttc ttcaaagcga gcgtatatat taatggtact taatccggga atgtaataca
     1321 cctttgcggc atctgttaca tattccttca tttttttatc ctttttggtc ttaccatcat
     1381 attgctttac ataatcctgc cagatatcct cgactttcga tgttttcagg tcatttaccg
     1441 acagactgag agaatttttc attcccattg acataggccg ctcagcttca gaatactgtg
     1501 cagtggctgt aaaacttaaa ctaataaatg taagtaaggt taagatgatg tttctcattt
     1561 ctgtttgttt tatgaataac ttattttcaa attcaatgtt gccttcaggt aattatccca
     1621 atttatacaa atataaaatg ttatacagac tcaacagcaa aaatctttag ttttttagag
     1681 caattccgga tatttacagg tgtatatccc aggaattcgc catctgcttc gatcattact
     1741 tctttattgt ttagactgcg aatttctatg cggtcacatt caaaccctgt tacattcgga
     1801 tggttcaaaa gtgaaccgtt gtaaaatttg ggtgtcaaga gtacgacttc ccattttggc
     1861 atgctatctg ccatggtaac cgccagtttt cctgaatcat aaacagcatg tggcaccagc
     1921 tgcatattgc cacctgagta ccgacactga ccgatgttga tcgtatagac tgtcttggta
     1981 aagtctttgt cgtcagcgcg gattcttact ttttcgggct tgtatttcaa aagattttta
     2041 aacacgtgca acagatatag cttacttgca agacctgttt tcttgtattt ctcagtagct
     2101 ttcagcacaa aagcatcata agccagacct gccacattga taaaataacg gctttgctgt
     2161 ttatagtttt cgtcgtaata atctataagc cctacatctt ggatatgctg tcgatcatct
     2221 tgcaagcttt tgacgaattc aacagccctg cgagaaatcc gatgttctct tacccagtcg
     2281 tttccagtac cactggagaa aagtgtcatg gcagtttctg taccaaatcc ttcatcctga
     2341 gcctgaagga taccattggc aacttcattt gcagtgccgt ctccgcccac ggctattatg
     2401 cgcttgcttc ctttgcaggt caatcgtttg gctatatcga ttgcatcacc ggtacccttg
     2461 gtaaaatatt cctgataacc gatgttagaa ttatcaaatt ctctttggag actcgcccat
     2521 gctttcacag attggccggc cccacccatc ggattaataa ttatatcata tttcatcaac
     2581 agtacaattt aaactattgg tattcatcgc tgaaaataca aagcttgtct aaataatatt
     2641 caatcaagta aaattatatt gtattttagc caccgaatta tactcaaaac cagaaaatgg
     2701 atatactcaa catcagaaat gtagtcaaat cctacgaatc aaaagtggct gttgacgacg
     2761 tcagtctgaa tataccacaa ggcattatct ttggtatgct cggacctaac ggggcgggta
     2821 aaacatccct tatacgcatc attactacca tcaccagacc ggatagtggc aaaatccttt
     2881 ttgacaatga cccactcatc gaaaagcaca cggaacaaat gggctacatg cctgaagaac
     2941 gtggattgta caaaaaaatg aaggtaggtg aacaacttct ttacctggct cgtcttaaag
     3001 gactctcaaa acaagaagcc gaatcaacca tagcccattg gtttgacaaa tttgacatca
     3061 aagattggta caacaaaaaa gtgatggatt tgtcaaaggg aatgcaacaa aaagttcagt
     3121 tcatcgctac ggttatgcac cgcccaaaac tcatcatact tgacgaacca ttctctggcc
     3181 tcgaccccat caatgccaaa ctgatcgaga atgaaatcat caggatgcgt gatgaaggca
     3241 caagcatcat tttctcaaca cacagaatgg aacaggtaga agaaatatgc gacaaaatag
     3301 tactcatcaa tcaaggaaag aatgtacttg aaggagatgt taacgaaatc aaaaatactt
     3361 acaaggaaaa tttgtacaac atccgctacg aaggtaacat caacgtccat ttcgaacaat
     3421 ttggtgaaat aacacataaa aacgataaca gcattaccat aaaatgcgat tcgtcacaaa
     3481 aagcaaatga tttgctcaaa tggatgatcc aaaacggcgc acttatcaat tcattcaagg
     3541 agatactgcc taccatcaat gatatattta tcagaaaagt agaaggcaac atttaaataa
     3601 aaaaacaata aagcaatgga caagctttgg ttaattatac aaagagaatt tctgacaaga
     3661 gtgaaatcaa aatcattcct gttatcaact tttttgactc cgctggcatt tctggtattt
     3721 tttgttatag ccggatttat catgagctac aaggaaggtg aagataaaaa ggtagtagtt
     3781 tctgatccgg ccaatctgct cgacaaggta ataaaagatg aaagcaatat ttattacacc
     3841 ttctcggatg aaagcatcga caatctgaaa aaacaggtcg aacaaggcaa ttactccggc
     3901 attctcgaaa tacccaaacc cgacagtgct gctatccgtc agctaaaggt ttattattat
     3961 tctgaaaatc agctcggcct tgatgatcag gctgccctcg aaaaccaact cgaagaccgc
     4021 ataggtgaat ataaactcaa agcacagggc ctggataaat ctattcttga aaacctgaaa
     4081 acaaatataa gtattgaccc tgaacccatt aatgccagcc aaaaaaacga aagctcgtct
     4141 gctgctgcca ttggtgccgc acttggtact atcatgggca tcatcatgta tatgtcggta
     4201 ttcatttatg gttcaatggt gatgcgtagt gtcatggaag aaaaaatgag ccgcatagtg
     4261 gaagtcatga tttcgaccgt caaaccattc caactaatga tgggcaaaat aattggtgta
     4321 ggtgcagtag gcctgacaca atttatactt tggatgatac tcattccctt aatcagtatg
     4381 ttcgtaacca tggtattcgg cttcgatccc cagcagatgc aaagccaggc tatgaccggt
     4441 gctcaatcgg ttgacccgga tgatcttcaa atgatggtat caaaaatatt catcgaagtt
     4501 aacaacatga actggtggct catcatcccg agttttatat tttactttct gtgcggttac
     4561 atcttgtatt cgtctttgtt tgctgctgta ggttcggctg ttggagacga tcttggagaa
     4621 agccaatcac tgacactacc gataaccata cccgtactgc ttgcatttta tatcatgttc
     4681 gcagcggtaa gggcaccaca gagcagtatg gctgtatttg gttctatatt tccattgttt
     4741 tcacctattg taatgccgag cagaatagta ttcgacgtgc ccgcctggga aatagccgtg
     4801 tctatgctgc tgctcctggt tactacaata tttatggtgt ggctggcagg taggatttac
     4861 cgcgttggta tccttatgta cggaaaaaaa gcaagcttca aagaactgag gaagtggatg
     4921 ttttataaag attaacaagc atatttttca gcttcgtgag ttgatttcct tccaggtttt
     4981 gtacaatgtc tcctgttcag gtctgtctgc gggcatttca cgcaatggtt cacagggtat
     5041 gagttgccca tagcattctt cgggtaatct ttgatacagc gcagtgccgg cagatttcca
     5101 gtcaatcatg tcgtcactca cttgcttgat gattttcgcc gggctcccta ctacgagact
     5161 ccggtcaggg atgatcattt cggcaggaac gaacgtcaat gcaccgatta tacattcatt
     5221 ccctacctgg gcattgtcca tgatgacagc attcatacct acgagtgtat tacgccctat
     5281 actggcaccg tgtacgatgg caccatgacc tatgtgtgcc gcttcgcaca gatataccgt
     5341 cacgcccggg aacatgtgga tggtacagtt ttcctgcaca ttgcacccgt cttctatgac
     5401 aatgccaccc cagtcaccgc ggatggctgc acccggacca acatacacgt gcctgccgat
     5461 gacaacattg ccggtgacac atgcttgagg atggacaaaa gccgtttcgt gtactacggg
     5521 tacaaatcct ttgaaagcat aaaacataag caggtgttat tgtgaaatat ccgaatctcc
     5581 tttcgtgttc ttaagccccc tgtatttttg catagcttct tttatgcgtc ccatgcggtt
     5641 ttcgggactt gggtgcgtac tttgaaactc aggtacacgc tgtccgcccg acgattgttc
     5701 gagtattttc attactccaa gcaagccttc gggttcgtag cctgcctcga gcatgaatct
     5761 gacaccgagg tcatccgact gcagttcttg accgcgaccg tatttcatgt ttatcatatt
     5821 gccaatcatc tgagccattt gagccgtgct ctggctgccg gtagctacca ctgccgctcc
     5881 tgttaagcca tctgtgagtt gttggtcggc gatgcgttgt gccgaatgcc tggctaccac
     5941 atgaccgatc tcatgaccaa gtacacctgc caactgatct tcggtctcga gctgtgaaaa
     6001 caaagcgtag gtaataaaaa tctggccacc gggcaaagca aaagcattga taacattggg
     6061 gtcggcgagc aaatgaaaat cgtactgata aggtgttttc ttagcagcag aatttcttac
     6121 cacattctgc ccgaccttct ttaccaattg ttgtgcacgc tcgtccggat aaagacctcc
     6181 gaattcctga gccattgcag gtgcactctg caagcccatt gctatctcct gctcaggcga
     6241 aatgccgata tgctgttttt ctttggtaat aggattcact tcagtttttg aataatactg
     6301 aaacaaggtg aacaaagcca ttatcagccc cagaataagt gttccaccta ttctcgaccc
     6361 gccaccggat gaattctgga atattgacat tttacttaat tgttattgtt taataatgtt
     6421 atgacgataa attatttctt ttcgtcacct caaatataca ttttatgaag aatttaatac
     6481 tacaccaaca cagtagaatt ttcaatattt cattaaaaaa atatcatttg ccttcgatta
     6541 acatgaatat tcctccattg acatacatat tattgaataa atactaaaaa attgaacccg
     6601 gatcatgtta taaaatgatt gattcgttat atttgtcagt aatattattc caccattacc
     6661 caaaattaaa aattaatgaa aataaatatc agcaataaga atatgccaac agtgatgttc
     6721 attgcatttc tcagtatgtt gtccaattca ctttttgctc agcaggatgt ggtcgaaaat
     6781 tatacagctg ttgatactat cgccgttttc gatccggaaa caaacaaaga agttgtaagt
     6841 tacgtaaaaa cagaactgca agtacattca cgcccggacg aaatgccctt catgagtaa
//
LOCUS       DAOQQY010000147         6860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4615_length_6860_cov_5.538722, whole genome shotgun sequence.
ACCESSION   DAOQQY010000147 DAOQQY010000000
VERSION     DAOQQY010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4615_length_6860_cov_5.538722"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1044)
                     /locus_tag="PLE23_07925"
     CDS             complement(94..1044)
                     /locus_tag="PLE23_07925"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF009251.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMX83228.1"
                     /translation="MAEYNTISETDFHFPGQTGVYKGKVRDVYFTDDYIILIATDRIS
                     AFDHILPRAIPAKGQILTKMAAYFLEKTASDVPNWFIASPDPNVTIGKKCQAYKIEVV
                     VRNYLTGHAWRTYKAGERTLCGVTLPDGLKENQKFDHPIITPSTKADEGHDMDISREK
                     IIETGLVSEQEYTIIEQYALRLFGIGTSMAAERGLILVDTKYEFGKDKDGNILLIDEV
                     HTPDSSRYFYADDYQERFAQGLPPRQLSKEFVREWLIAHGFQGQEDQIMPVMSDTFVH
                     EITERYLELCRIITGEEPPFNSEPPAERMLRNVTNWLLVH"
     gene            complement(1102..1533)
                     /locus_tag="PLE23_07930"
     CDS             complement(1102..1533)
                     /locus_tag="PLE23_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83229.1"
                     /translation="MSLREKVNLFIYRYAEKGLEIFLMKSEAEDLLHLPNSSGNIAPI
                     QQIGKEKSLIQLDETTNPEGNIEINVAFEAEYHEIPSLKHLLKSDMNFVADKIIHMLP
                     DSEKGRFIALKEAIKKMRPHQNQALKELKDILVEKNLTKYL"
     gene            complement(1655..2326)
                     /locus_tag="PLE23_07935"
     CDS             complement(1655..2326)
                     /locus_tag="PLE23_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83230.1"
                     /translation="MSSIFVIGTLWLILAGVIIAILLKMWNAVAEFFVFNKNPEQFLH
                     ELYTTLEPYRASLPELNMEELSLLSSNTNLVQTKLLGQDTKAGYIRNVYQENLAIYAF
                     RQIGVNKRLFVFADKTGIFAFRELSKGTFRVFDERVVALGDYNPESGFIWKASAIGNL
                     DKDAEGRLTTMRIGQKQVANFVKKDNLSKVPQRHFSFIQNDEITRDTTFRILLFASLM
                     DSVQT"
     gene            complement(2354..3217)
                     /locus_tag="PLE23_07940"
     CDS             complement(2354..3217)
                     /locus_tag="PLE23_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HMX83231.1"
                     /translation="MRQLKITNKITTRESLALDKYLNDIGKISMLSSEEEADLSRRIK
                     EGDQEALDILTKSNLRFVVSVAKQYQNQGLSLSDLINEGNVGLMKAAKRFDETKGFKF
                     ISYAVWWIRQSILQAIVEYSRIVRLPLNKVGSYNKVNETFLSFIQEFEREPTNEELAE
                     LLDMTPKEVANMLRGTVRHVSVDAPITSDDSDSTMLDVISLNDDSPDQYLMDQSLKDE
                     VQQGLSILSPREVEVLSAYYGLNGFKALTLEEIGELFNLTRERVRQIKERAIRRLRKS
                     YNRNALRSYLG"
     gene            complement(3477..5624)
                     /gene="pnp"
                     /locus_tag="PLE23_07945"
     CDS             complement(3477..5624)
                     /gene="pnp"
                     /locus_tag="PLE23_07945"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HMX83232.1"
                     /translation="MGQQIPTTHSFNLPDGQTVTIETGKLAMQAHGSVVVRVGDMMIF
                     AAVVSNTEMKADAGFFPLSVDYQEKFASAGRIPGNFFRRETRLSEYEILISRLVDRAI
                     RPLFPDEYKNETQVTINLISADKEILPDAYVALATSAALTLSDIPFAGPISEVRVACI
                     DGQFHINPTRSNLEKADINIIVAATKDNVVMVEGEANECQESEMVDAIRVGHEAIKTQ
                     IDAQLELAKKFGSLDKPKRTVEPAPENEEIKNKVYELAHDKLNQIAHSFLEKQVRKSQ
                     FEEVKAAVTETLLAEKGEEYMAENGKFVSVYFDKLKKDVVREMVMAEKVRLDGRKPNE
                     IRPIWCEVDYLPSPHGSSIFTRGETQALATVTLGSKLDEMMMDSAFELYFEKFILHYN
                     FPAFSVGEVRPARGPGRREVGHANLAARSLRKVLPPELPYTVRIVSDVLESNGSSSMA
                     TVCSGSMALMDSGIQIAAPVSGIAMGLISDGKRNAILSDILGDEDALGDMDFKVTGTA
                     KGICGCQMDIKIEGLSFELLTEALNQAKEGRLHILGKMSEAIEHSREDYKPHAPRIEE
                     IIIDKSFIGAVIGPGGKIIQEMQKLTGTIITIEEKNEKGYIQIVSSNKDGVELAKEKI
                     ANIVFVPEVGQEYDAVVESLMPFGAFAKFRNASGLVHISEISHARVENVADVLKVGQS
                     IRVKLIEIDQKTGKYRLSMKALQPRPEKPANKH"
     gene            complement(5765..6040)
                     /gene="rpsO"
                     /locus_tag="PLE23_07950"
     CDS             complement(5765..6040)
                     /gene="rpsO"
                     /locus_tag="PLE23_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HMX83233.1"
                     /translation="MAVYLNSEKLQGIFKEYAGSEKNTGSTESQIALFTYRIQSLSEH
                     LKDHKKDHSCRRTLLTLVGKRKKMLQYLQGKDLTKYRSLISQLGLRK"
     gene            complement(6143..6601)
                     /locus_tag="PLE23_07955"
     CDS             complement(6143..6601)
                     /locus_tag="PLE23_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83234.1"
                     /translation="MLKIITTCILALVSLACMNAQTEMAWSTYNIGFVAPPTMQTLVD
                     SKGRFEVSNDNVQISIFPWDGRVNFDDLGDGTIDIATQLGYETTGSADALELEDFQGF
                     YVQATKQGVEAIVVLLYNNAGNVTLVADIQYQPGFINEAMDIALSFYATN"
     gene            6767..>6860
                     /locus_tag="PLE23_07960"
     CDS             6767..>6860
                     /locus_tag="PLE23_07960"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HMX83235.1"
                     /translation="MINLISDTVTRPTPAMLNAMYRAEVGDDVFR"
BASE COUNT         1905 a         1425 c         1355 g         2175 t
ORIGIN      
        1 aaagccttta cataatgttg cccatactcc tcaaatattt tttcgctcca actcgatttt
       61 ggcagtcgga acaattaaaa aaatatcgta taattaatgt accaacaacc agtttgtcac
      121 atttcttaac attcgttcgg caggtggttc actgttgaat ggaggctctt cgccggttat
      181 tatcctgcac agttcaagat agcgttctgt tatttcatgg acaaatgtat cggacattac
      241 cggcataatc tgatcctcct gtccctgaaa tccgtgagct atgagccatt cgcgaacgaa
      301 ttctttcgac agttgtcgtg gaggcaaccc ttgtgcaaag cgttcctgat aatcatctgc
      361 ataaaagtac cgcgagctat cgggtgtgtg aacttcatca atgagcaaaa tattgccgtc
      421 cttgtctttg ccaaactcat acttcgtatc aacgagaatc agtccacgtt cggcagccat
      481 acttgtaccg atgccaaaca aacgtagcgc atactgttcg ataatagtat attcttgttc
      541 agatacgaga ccggtttcaa tgattttttc tcttgaaata tccatgtcat gaccttcatc
      601 tgctttggtg gaaggtgtga tgatcgggtg gtcgaatttc tgattttctt tcagcccatc
      661 gggcaatgtt actccgcaga gtgtacgttc accagctttg taagtccgcc aggcatgacc
      721 ggtcagataa ttccgcacta ctacttctat tttataagcc tgacactttt taccgatggt
      781 aacgtttgga tcaggactag cgatgaacca gttaggaacg tctgaagcag ttttttcaag
      841 aaaataggca gccattttgg taagtatctg acctttggcg ggtatagcgc gggggagaat
      901 gtgatcaaat gccgaaatgc ggtcggtggc tatcagaatg atataatcat ctgtgaaata
      961 aacatcacgc accttgcctt tatagacacc ggtctgtccc ggaaaatgaa aatctgtttc
     1021 agaaattgta ttatactcag ccatagctat tcggaatatt gccggcaaag ttagctaaaa
     1081 gggagcgagt taaaactaaa atcagagata tttggtcaaa ttcttttcaa ccaggatatc
     1141 cttgagttct ttcaaggcct gattttggtg ggggcgcatt tttttgatgg cttctttaag
     1201 agcaatgaac ctaccctttt cagaatccgg caacatgtgg ataatcttgt cagccacaaa
     1261 attcatatcg gacttgagca gatgcttgag tgacgggatc tcgtggtact ctgcttcaaa
     1321 tgccacgttt atctctatat ttccttcggg gttggttgtt tcatcaagtt gtatgagact
     1381 tttctctttg cctatttgct gaattggggc aatgttaccg gacgaatttg gcagatgaag
     1441 caggtcttca gcttcgcttt tcatcaggaa tatttcgagg cctttctctg cgtagcggta
     1501 aatgaacaaa ttgaccttct ctcggagtga catggttgat atttttgtag tgttaagata
     1561 ctaacattca aagataaagc atgttgtgca aaaaattaat tttaataaaa aataatttaa
     1621 aaaaaaatag atcgtgctca tcaaaagaag taaactatgt ctgcactgaa tccatcagcg
     1681 aagcaaacaa cagaattctg aaggtggtat cacgggtaat ttcatcgttt tgaatgaagc
     1741 tgaaatgtct ttggggcacc tttgatagat tatccttttt aacaaaattt gctacctgct
     1801 tctgaccaat acgcatggta gtaaggcgac cttctgcgtc tttgtcaaga tttccgatgg
     1861 ccgaagcctt ccagataaaa cctgattcgg ggttataatc acccaatgcg acaactcttt
     1921 catcgaatac cctgaatgta cctttagaca attcgcggaa tgcgaaaata cctgttttgt
     1981 cagcaaaaac aaacagcctt ttgtttacac ctatttgccg gaatgcatag atagcaagat
     2041 tttcctggta aacattcctt atatagcctg ctttagtatc ttgtcctaac aattttgtct
     2101 gtaccagatt ggtattggaa gataagaggc tcagttcttc catgtttagc tcaggcaatg
     2161 acgctctgta aggttcgaga gtggtataaa gctcatgtag aaactgttcg ggattcttat
     2221 tgaatacaaa aaactctgca accgcattcc acattttcaa caaaatggca atgatgacac
     2281 cggccagaat caaccaaagt gttccgataa cgaaaatgga actcatgata aagttttggc
     2341 aaatgtagga aaattatccg agatatgaac ggagtgcatt ccggttgtac gatttgcgta
     2401 atcttctgat ggcccgttct tttatttggc gtaccctttc gcgagtgaga ttaaataatt
     2461 ctccgatctc ttcaagtgtc aaggctttaa agccatttaa gccataataa gctgacaaca
     2521 cttctacttc gcgtggtgat agtattgaaa ggccctgttg cacttcatcc ttgagtgact
     2581 gatccatcag atactggtct gggctgtcat cattcagaga tataacatcg agcatagtag
     2641 agtcagaatc atcggaagta ataggtgcgt caacggatac gtgcctgaca gtaccacgaa
     2701 gcatattggc aacttccttt ggggtcatgt caaggagctc ggcgagttct tcattggtgg
     2761 gttcgcgttc gaattcctgt atgaacgaaa ggaatgtttc atttactttg ttataagaac
     2821 caactttatt gagaggtagt cttacgattc ttgaatattc tacaatagcc tgcaggatgg
     2881 actgtctaat ccaccacaca gcataggata taaatttgaa tccttttgtt tcgtcaaatc
     2941 ttttggcagc cttcatgagg cctacatttc cttcattgat aaggtcgctt agtgaaagtc
     3001 cttggttttg atattgtttg gccactgaga caacaaagcg cagatttgac ttagtaagaa
     3061 tgtcaagtgc ttcttgatca ccttccttta tgcgtcgtga caaatctgct tcttcttcag
     3121 atgacagcat tgaaatttta ccaatatcat tcagatattt gtcaagtgct aaactttcac
     3181 gtgtggtaat cttattggta atttttaact gacgcatgtt cgggacattt ttctaagaat
     3241 gattataata ataagacgtc agagcaaaaa aaaagtttca aaatatttga ctttatcatc
     3301 aatacagatt aatatcgccg tatatttttc acacctctcg acctttggca attgttgcaa
     3361 tatctatgcc aaaggaatgt tgttagttct ataaagaatt gcatgaatcg aaactcactt
     3421 taacaaaaaa gcctgctgat gaatcaacag gcttattatt atatttttga agcaaattaa
     3481 tgtttgttag ccggtttttc tggtcttggc tgcaatgctt tcattgacaa acggtatttg
     3541 cctgtttttt ggtctatttc tataagtttg actcttattg actgtccgac cttcagtaca
     3601 tcagctacat tttcaaccct tgcgtgagat atttcagaaa tatgtacaag acctgatgca
     3661 ttcctgaatt tagcgaaagc gccaaaaggc ataagacttt caacaacagc atcgtattct
     3721 tggccaacct ccggtacaaa aacgatatta gctatctttt ctttggcgag ttcgacaccg
     3781 tctttgttgc tgctcactat ctgtatgtaa cctttttcgt ttttctcttc gatggtaatg
     3841 atggtgccgg tcagtttttg catttcctgg atgattttac cacccggacc tatcacagcg
     3901 cctataaagc ttttatcgat gatgatttcc tcaatacgtg gtgcatgagg cttgtaatct
     3961 tctcttgagt gttctattgc ttcgctcatt tttccgagaa tatgtagtct tccttcttta
     4021 gcctgattga gagcctcagt cagaagttca aaagacaagc cttcgatctt gatgtccatc
     4081 tggcagccgc agataccttt agccgtacct gtcactttga aatccatatc accaagggca
     4141 tcttcgtcac ccagtatatc actcagaata gcatttcgtt tgccatctga tataaggccc
     4201 atagcaatac ctgataccgg agcggcaatc tggatacctg aatccatcaa ggccattgaa
     4261 cctgaacaga cagtggccat agaagacgac ccgttagact ccagaacatc cgaaacgata
     4321 cgaactgtgt aaggaagctc tggtggaagt acttttctca atgaccttgc agcaaggttg
     4381 gcgtgcccta cttctcggcg ccccggacct ctggccggcc ttacctcccc tactgaaaat
     4441 gcagggaaat tataatgcag gatgaatttc tcgaaatata attcgaatgc actgtccatc
     4501 atcatttcgt cgagtttgct acccagtgta acagtagcca gtgcctgggt ttcgccacgt
     4561 gtgaagatag acgaaccatg tggagatggc aaataatcca cctcacacca gataggacgt
     4621 atttcatttg gttttctgcc gtcgagtcgt actttttcag ccattaccat ttcacgtact
     4681 acatcttttt taagtttgtc gaaatataca gatacgaact ttccgttctc tgccatgtat
     4741 tcctcacctt tttctgcgag cagggtttct gtaacggcag cttttacttc ttcaaactga
     4801 gatttacgta cttgtttttc taagaaagag tgagctattt ggttaagttt atcgtgtgcc
     4861 agctcataca ctttgttctt gatttcttca ttttcaggtg ccggttcgac tgttctttta
     4921 ggtttgtcga gagaaccgaa ttttttggca agttcgagct gagcgtcaat ctgtgtttta
     4981 atggcttcgt gcccgacccg gatggcatca accatttcag attcttggca ttcgtttgct
     5041 tcaccttcta ccattacaac attgtctttt gttgcagcca caattatgtt gatgtctgct
     5101 ttctcgagat ttgatcttgt tgggttaatg tgaaactggc catcgataca ggcaacgcgt
     5161 acttcagaaa tcgggcctgc aaaaggaata tcagacaagg taagtgcagc agaagtagcc
     5221 aatgccacat aagcatcagg caatatctct ttgtcagctg agatcaggtt tatggttacc
     5281 tgagtttcgt ttttatattc atccggaaac agcgggcgta ttgctctgtc cacgagtctt
     5341 gaaataagga tttcgtattc actcaggcgt gtttctcttc tgaagaaatt gcccggaata
     5401 cgacctgcag aggcgaattt ttcctgataa tcgacagaaa gagggaaaaa accggcatca
     5461 gctttcattt ctgtatttga aacaacagct gcgaatatca tcatatcacc gaccctaacc
     5521 actaccgagc cgtgagcctg catggcaagt tttccggtct caatagttac tgtctggcca
     5581 tcaggaaggt taaatgaatg tgtagttgga atttgttggc ccatgatttt tgttttagat
     5641 tttttacaat agcgcaataa tgtttgctgg ttgcaaaaaa gaagaaaaat tgttgattat
     5701 aacccaaatt actgaaatga gataacagaa aaaaagtgct tctgaggcag aagcactttt
     5761 ttaattattt cctcaaaccg agctggctta tgagtgatct gtattttgtg aggtctttgc
     5821 cctgcagata ttgtaacatc ttctttcgtt tacccaccag ggtaagaagt gtccttctgc
     5881 atgaatgatc ctttttatgg tctttcaggt gctccgacaa actttgaatt ctataagtga
     5941 ataatgcaat ctgagattct gtagagcctg tattcttttc agaaccagca tattctttaa
     6001 aaatcccttg caatttttca gagttgagat aaactgccat tcttctttag ttttgctact
     6061 tttttatttt tactttttcg aagtgcaaaa ttaagacaat ttattgaata ataaaatact
     6121 tatgtaaaaa aaaataaaaa tattaatttg tagcataaaa actaagggca atatccatcg
     6181 cttcattgat aaagcccggc tggtactgta tatcggctac caaggtaaca ttgccggcat
     6241 tattatacaa gagtactaca atggcttcaa cgccctgctt ggtggcctgc acatagaatc
     6301 cctgaaaatc ttcaagctct agtgcatcag cgctgcccgt agtctcatag ccgagctgag
     6361 ttgcaatatc tatcgtacca tcacccagat cgtcaaaatt tacgcggcca tcccatggaa
     6421 aaatgctaat ttgtacatta tcattactta cttcaaaccg acctttgcta tccaccaagg
     6481 tctgcatagt cggaggcgca acaaaaccaa tgttgtaggt gctccatgcc atctctgtct
     6541 gtgcattcat acatgctaat gacacaaggg ctaatataca agtggtaatt atcttcagca
     6601 tctcagtgaa aatttcatga agagaaatat cgttgtaaaa ttaacaatta ttctctccaa
     6661 aagatttcag tagcgaataa ttatttttat aagtctaaat ttgcctcctg aaccaacatt
     6721 tcattttaca ttttcatata ctacagatta ataaatatat atataaatga tcaacctgat
     6781 aagcgatact gttacccgtc ctactccggc tatgctcaat gccatgtacc gtgccgaggt
     6841 aggtgacgat gtattccgcg
//
LOCUS       DAOQQY010000148         6834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4647_length_6834_cov_5.376899, whole genome shotgun sequence.
ACCESSION   DAOQQY010000148 DAOQQY010000000
VERSION     DAOQQY010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4647_length_6834_cov_5.376899"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLE23_07965"
     CDS             complement(<1..874)
                     /locus_tag="PLE23_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83236.1"
                     /translation="MQTRKTKIITLIIGIWAVTLFSLFGQVTVTLNGPNYVPVGANCK
                     TSFSATKNDFTFTANGCMLDLDNDVTVSQDKTVVPAGDNITVTVDVNSPGCPPNQFVF
                     ILPAKDITAPVFKVQPTPITEIVCQGSGNQTFFNSWLATHGGAVIEDNCTASGNIVWT
                     TDPANPQMPTDYCTPGEVSVFFKATDKGGNVVSSNPAVFRIIDNKAPNIISKPFPVSF
                     ECNKSTNYNTELTNWVNTLAMNQAKVSDECISVNNISWSVKIDNGPEHSIPTQLTIDS
                     KKCNVSKSIVFIARD"
     gene            1485..3509
                     /locus_tag="PLE23_07970"
     CDS             1485..3509
                     /locus_tag="PLE23_07970"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HMX83237.1"
                     /translation="MTLTEFQKSILVGIPDELPEKKPYDHSVSHAPQRNIKGLLSREE
                     KQLAVRNALRYFDKKFHKILAEEFADELNTYGRIYMYRFRPDYEMKARDINDYPARSQ
                     QAAAIMLMIQNNLDYKVAKHPHELITYGGNGAVFQNWAQYRLVMKYLAEMTDEQTLVL
                     YSGHPLGLFPSSKNAPRVVVTNGMMIPNYSSKDDWNKYNALGVTQYGQMTAGSFMYIG
                     PQGIVHGTTLTLLNAGRLNGVGQDDLRGKVFVTSGLGGMSGAQSLAAVITGAVGVLAE
                     VDPDAVRQRITDGYIKAENVYTDLNLLISRIRECRENKTAITLVYAGNVVDLWQAIYD
                     QNLQVELGSDQTSLHNIDDLGYAPVGYSFEEAKQLLNTDKTKFMQEVRKTLRLHVDLI
                     NKICERGMYFWDYGNAFLKEAGIAGADIWLDKERDLFKYPSYVQDILGPLCFDYGFGP
                     FRWVCTSGKKSDLDTSDRIAAQVIEEIMKTAPYEIMPQLHDNLIWIQKADENIPVVGS
                     KSRILYADAEGRIKIASAFNKAIAEGKITAPVVLGRDHHDVSGTDSPYRETSNIYDGS
                     RFTADMAVHNVIGDSFRGATWVSLHNGGGVGWGEVINGGFGMVLDGTPDAQHKLESML
                     HWDVNNGIARRSWARNKEAMFAIKRAMDDNPNLKVTLPNLVDDDLLSTLF"
     gene            3572..>6834
                     /locus_tag="PLE23_07975"
     CDS             3572..>6834
                     /locus_tag="PLE23_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HMX83238.1"
                     /translation="MKFIMKYFPVLVLVCLMQFTLTAQMVTGQDTLYGNEWIKPGKEY
                     YKFPVFQDRIYRIPYAVLQAAGVPVNSVQGKNFQLYRMGKEVSIFTTTDSLLSENDYI
                     EFYGQQMRSEIDKFLYNNPEKDLLNPEYSLINDTIYYFLTWENTASVNRLISIPNNIN
                     QPGKLIEDVFTTSVFSFPNSRIKAGDADAGIFSSFDTGEGYGTGVSKTFSAPLTIQSV
                     SPNEDSISIHIRVYGGIGDHNLSLYLNDQLFANKFFKALSILDTTFQVPVGLLAASNT
                     IKINDDGASLKGFSVATVQIGYQRSLIYSGFTQNRFEIPASADTSYLEIQGFFQGGGS
                     PLLYNMTKAYRMTGEVSNGITRFNLPPSSKTQDILLHSGATPLAVTALTKIVFKDYSK
                     LNADFVIISHPALYDDGNGNNFVQQYADYRASDAGGNHSVVIVDIQDLYDQFAYGNVR
                     HFISMRNFAHFIKKNWSAAKSLFIIGKGREYAYFRQAGHLTNPSNSSFYVPTFGTPGS
                     DNLLVMSQGEYSPVLALGRIAAQTPQDIADYLKKIQDYESFGNNQQTIADQLWKKEII
                     HLGGGKNKNEQNSFRNLLESVVPVITNNRFGGRVHGFYKTSTDPIQVSQNEQIFDLIN
                     NGVSIITFLGHSAVGTFDFSIDNPSNYQNFGKYPLMFSLGCYSGNYHTPSRGTGEGFC
                     FYKEKGALGFVATSFSGYPSVLTQFAGEFYRQQGGDSYGQSVGEVLQHSYSELAKNAT
                     AYTKLLIEQMSYHGDPAYTIHTAPGPDFTPDISTLKMEPAIVTAQTDSLSISFDVANI
                     GEYIEDSINITLRQKLPAGEIRTLADFGIKTPSFRTPIHVKVPSFKAEAFGQNILYID
                     VDTDNKVVELPSPLAEQNNSLSQNGETGFMFFVTDNSAIPVSPPNYSIVSNEDFELIA
                     CTADPLAKEKPYLIQIDTTRLFNSPRLASNTIATKGGLIEWVPGINMLDSVTYYWRIT
                     PDSTQTGVGAIWQYSSFFYKENSPKGWSIAHNFQFNEGNLVNIKPVASDQKMYFITDI
                     KDVFIQNFKRQGELYPAYYINNGLTEINYGNDIAAGVYVGVIDPLTGEVLIPANTELG
                     "
BASE COUNT         2000 a         1358 c         1508 g         1968 t
ORIGIN      
        1 cgtcccgagc gatgaacacg atggattttg atacattaca ttttttactg tcaatagtaa
       61 gttgggtagg aatggaatgc tccgggccat tgtctatttt tacactccag cttatgttgt
      121 tgacgcttat acactcatct gatactttag cctggttcat tgcaagcgta ttgacccagt
      181 tggttaattc agtattgtag ttggttgatt tattgcattc aaacgatacc gggaaaggtt
      241 tgcttataat gttgggagct ttattgtcaa tgattctgaa cacagccgga tttgagctga
      301 caacattacc gcccttgtcg gtcgctttaa agaaaacact cacttcacca ggcgtgcaat
      361 aatcagtcgg catttgtgga ttggcagggt cggttgtcca gacaatgttg ccggatgctg
      421 tacaattatc ttcaatgaca gcaccaccat gtgtagcaag ccatgaatta aaaaacgtct
      481 ggttcccgga gccttggcaa acgatttcgg tgatgggtgt aggttgtacc ttgaataccg
      541 gagcagtaat atctttagcc ggaagaataa aaacgaactg gttcggaggg catcccggtg
      601 agttgacatc tacagtcact gttatgttat cacctgccgg cactacagtt ttgtcctgcg
      661 agacagtaac gtcgttgtca aggtctagca tacagccatt ggcagtgaaa gtaaaatcat
      721 ttttggtggc agaaaagcta gttttgcaat tggcaccaac cggtacatag tttggaccgt
      781 tcagcgttac agttacttgg ccgaacagag agaatagagt gactgcccag ataccaataa
      841 ttaaagttat aatcttggtt tttctcgttt gcatgtgtgg tttcttttgg ttgacatatt
      901 caccgatggt cattatgctt atttcaataa atgcataaga caatctggta gaaaaagtat
      961 tttacacggc tgaaattggg gtgcgatgtt tggtactgtt cgtttgcaaa tgtgttttac
     1021 attttgcatg atgttgttcg gttttttgaa ggactcacta tttgattagc caaaaatacg
     1081 gatatttatt taaatgtcaa aatccagcgc aaatatttta acacttaaca ttaagattgt
     1141 tgatatttta atttttaaac gataatgtta tttatcacaa attatttatt aatattatat
     1201 gataatttaa ttgttaagat gtgttcaatt tgaataaggt catatttgct ccttcaacat
     1261 aaatattaaa gcagtcttgc tgtagtctta cggaaatgtg gatgtcctca agcaaaagta
     1321 cagtaattat aaacgatatt ttattaataa tgcattactt tatggttcaa gttctttcaa
     1381 taagttggct tttcaagttt cataaacaat tcacactcaa aaaattgatt ttactttgct
     1441 ttaacagctg aaatgggtta aatttgttgt tgaaaatatg aattatgaca ttaaccgagt
     1501 ttcaaaaatc tattctggtg ggcattcctg atgagcttcc ggaaaagaag ccatacgacc
     1561 acagtgtaag tcacgctccg caaaggaaca tcaagggatt gctctcacgt gaagaaaaac
     1621 agcttgccgt aaggaatgca ctccgatatt ttgacaaaaa atttcacaaa atactagccg
     1681 aagaatttgc cgatgaactg aatacttatg gccgtatcta tatgtatcgc ttcaggcctg
     1741 actatgaaat gaaagcacgg gatattaatg attatccggc aagatcgcaa caagctgctg
     1801 ctatcatgtt gatgattcag aataaccttg actataaggt ggccaagcat ccgcacgagc
     1861 tgattactta tggtggcaac ggtgctgttt ttcagaattg ggctcaatac cggcttgtga
     1921 tgaaatacct ggccgaaatg accgatgaac aaacacttgt attgtattcg ggacatccac
     1981 tagggctttt cccttcttca aaaaatgctc cgcgtgtggt agttaccaat gggatgatga
     2041 ttccgaatta ttcatcaaaa gacgactgga ataagtacaa cgcccttgga gttacacaat
     2101 acggtcagat gacagcaggt agctttatgt acattggccc acaaggtatt gtacatggga
     2161 ccacccttac tttgctcaat gcaggcagac taaacggagt agggcaggat gacttgagag
     2221 ggaaggtgtt tgtaacctca ggcttgggtg gcatgtctgg cgcacagtcg ctggcagccg
     2281 tcattaccgg agcagtaggt gtgctcgcag aagtggaccc tgatgccgtc agacaaagaa
     2341 taactgacgg ctatatcaaa gcagaaaatg tatatacgga tcttaacttg ctgatatcca
     2401 gaatacgtga atgccgcgaa aataaaaccg ccatcacgct ggtgtatgca ggtaatgtgg
     2461 ttgacctctg gcaggcaatt tacgatcaaa accttcaggt agagctcggc tctgaccaga
     2521 cttcactcca caatatcgat gaccttggtt atgcacccgt cggctacagt tttgaggaag
     2581 ccaaacagct actcaatact gataaaacta agtttatgca agaagtgcga aaaacactga
     2641 ggctacacgt tgatcttatc aataaaatat gtgagcgggg aatgtatttc tgggactatg
     2701 gcaatgcttt tctcaaagaa gccggtatag ctggagccga tatctggctt gacaaggagc
     2761 gcgatttgtt taaatatcct tcttatgtgc aggatattct cggaccactt tgttttgatt
     2821 atggttttgg gccattcagg tgggtgtgta cttcaggaaa aaaatctgac ctcgatacat
     2881 ctgaccgtat tgctgcccaa gtaattgaag aaataatgaa aacggccccg tatgaaataa
     2941 tgccacagtt gcacgataat ctcatctgga tacaaaaggc ggatgaaaat ataccggtag
     3001 taggctcgaa atcacgaatc ctttatgcag atgctgaagg aagaattaag atagcaagtg
     3061 ccttcaacaa agccatagct gaaggaaaaa taaccgctcc ggtcgtactc ggcagagatc
     3121 accatgacgt ttcgggtacc gattcgcctt atcgcgagac ttcaaatatt tatgatggtt
     3181 cgcgttttac ggcggatatg gctgtgcaca atgttatagg tgacagcttc agaggtgcta
     3241 catgggtaag cctgcacaac ggcggtggag taggctgggg cgaagtaatc aatggtggtt
     3301 ttggtatggt actggatggc acgccggatg cacagcacaa actcgaatca atgctccatt
     3361 gggacgtgaa caatggtata gccagaagga gctgggcaag aaacaaagag gcgatgttcg
     3421 caatcaaacg cgccatggac gataatccca atctgaaggt gacattacca aaccttgtgg
     3481 atgatgattt gttgtcaaca ttattttaag acgggcaatg ttgcataata atatggtttg
     3541 tattgatgtt cttgtttggt aaaatttgtg tatgaaattt ataatgaaat atttccctgt
     3601 tttggtactt gtgtgtctga tgcagtttac tttgactgca caaatggtca ctgggcagga
     3661 tacgctgtat ggcaacgaat ggataaagcc tggaaaagag tattataaat ttccggtatt
     3721 tcaggataga atttaccgca taccttatgc ggttctgcag gctgccggtg tgccggtcaa
     3781 tagtgtacaa ggaaaaaatt tccagctcta cagaatgggg aaggaggttt ccatttttac
     3841 tacaacagat tcgcttcttt cagaaaatga ttacatcgag ttttatggtc agcaaatgag
     3901 gtcagagatt gacaaatttt tatacaataa tcctgaaaaa gacttgctca accccgaata
     3961 ttcgttgata aatgatacga tctattattt tctgacttgg gaaaacactg cgagtgttaa
     4021 taggcttatt tcaatcccaa acaatattaa tcagccagga aagctgatag aggatgtttt
     4081 tactacttcc gtattttctt tccccaatag caggattaag gcaggtgatg ccgacgcggg
     4141 aatattctcg agctttgata caggtgaagg atatggcacc ggcgtgagca agactttttc
     4201 tgcaccttta accattcagt cggtttcacc aaatgaagac agcattagca tccatattcg
     4261 ggtgtatgga ggtatcggtg accataattt gagtctgtat ctcaatgacc aactatttgc
     4321 caataaattt ttcaaggctc tgagtatatt ggatacaaca tttcaggtgc cggtcggtct
     4381 tctggcagca tctaatacaa taaagataaa cgatgacggt gccagtctga agggattcag
     4441 cgtggctact gtacagattg ggtaccaaag aagccttatt tattccgggt tcacgcaaaa
     4501 tcgcttcgaa atacccgctt ctgctgatac ttcatacctt gagattcagg gcttttttca
     4561 gggcggagga tcacctttgc tctacaacat gaccaaagca tacagaatga cgggtgaggt
     4621 ttcaaatggt ataacaagat ttaatctgcc accttcttcc aaaacacagg atattctgct
     4681 gcattccggt gctacaccct tggctgtaac agcacttaca aagatcgttt ttaaggatta
     4741 ttcaaagcta aatgctgatt ttgtaatcat cagccacccg gcactgtacg acgacggcaa
     4801 tggcaataat tttgttcagc aatatgctga ctatcgtgcg agtgatgcag gaggcaatca
     4861 ttcagttgtt atagttgata tccaagacct gtatgatcag tttgcctatg gcaatgtaag
     4921 acattttatc agtatgagga attttgccca tttcatcaag aaaaactggt cggctgcaaa
     4981 atccctgttc atcataggaa aaggtcgtga atatgcctat tttcgtcaag cagggcactt
     5041 gaccaatcca tccaacagta gtttttatgt tcccactttt ggtacgccgg gttcggacaa
     5101 tttgttagtc atgtcgcagg gcgaatattc gcctgtgctt gctttaggtc gtattgctgc
     5161 acagacacca caggatattg ctgattatct gaaaaagatt caggactacg aatcatttgg
     5221 caacaatcaa cagaccattg ctgaccagct ctggaaaaaa gaaatcatcc acctgggagg
     5281 tggcaagaat aaaaatgaac aaaattcatt ccgcaatctg ctcgaaagtg tggtgcctgt
     5341 catcaccaat aatcgttttg gtggtcgggt tcacggattc tacaagacca gtaccgatcc
     5401 tatccaggta tcacagaacg aacagatatt tgaccttatc aataatggtg tgtcaatcat
     5461 tacttttctc ggacattcgg ctgtgggcac atttgacttc agtattgaca atccttccaa
     5521 ttatcagaat tttggaaagt acccattgat gttttcactt gggtgttatt ccggcaatta
     5581 tcatacacca tcaagaggca caggtgaagg tttttgtttt tataaagaaa aaggcgctct
     5641 gggttttgtg gcaacgtctt tttccggtta tccttcggta ttgactcagt ttgcaggcga
     5701 attttacaga cagcagggtg gtgacagtta cggacagagc gtcggagaag tgctccaaca
     5761 ctcctacagt gagttggcaa agaatgctac tgcatacacc aagctactca tcgagcagat
     5821 gtcttaccac ggagatcccg cttataccat tcataccgca cccggtcccg actttacacc
     5881 tgatatcagt acactcaaga tggagcctgc catagtgaca gcacagacag actcactaag
     5941 catcagtttt gatgtggcca atattggcga atacattgaa gatagcatca acatcaccct
     6001 cagacagaaa ttgccggcag gcgaaataag aacactagct gatttcggaa taaagacacc
     6061 ctcgttccgt acgcctatcc atgtaaaagt gccttcgttt aaagcagagg catttggcca
     6121 aaatatcttg tacattgatg ttgataccga taataaagtt gtagaattgc cttctccgct
     6181 tgctgaacaa aacaattcgc ttagccaaaa tggtgagaca ggatttatgt tttttgtgac
     6241 agacaattcg gctataccgg tttcgccacc caattattca atagtcagta atgaggattt
     6301 tgaactcatc gcttgtactg ccgacccatt agccaaagaa aagccgtatc ttatacaaat
     6361 agatactacc cggttgttta acagcccacg tctggcatcg aatacaatag ccaccaaagg
     6421 aggcctgatt gaatgggtgc ctggtatcaa tatgcttgat tctgtgacat attactggag
     6481 aataacgccc gattctaccc aaactggagt tggtgccatc tggcaatatt catctttttt
     6541 ttataaggaa aattcaccaa aaggctggtc gatagcacat aattttcagt ttaatgaagg
     6601 caaccttgta aatattaagc cggtagcatc cgaccaaaag atgtacttca tcaccgatat
     6661 aaaagatgta tttatacaaa atttcaaacg gcaaggcgaa ctgtatccgg cctattacat
     6721 caataacggt ctcactgaaa tcaactatgg caatgatatt gcagcaggtg tgtatgttgg
     6781 cgtcatcgat ccgctcacgg gcgaggtgct catccccgcg aacaccgagc tcgg
//
LOCUS       DAOQQY010000149         6814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4674_length_6814_cov_5.181684, whole genome shotgun sequence.
ACCESSION   DAOQQY010000149 DAOQQY010000000
VERSION     DAOQQY010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4674_length_6814_cov_5.181684"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4002
                     /locus_tag="PLE23_07980"
     CDS             <1..4002
                     /locus_tag="PLE23_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83239.1"
                     /translation="VGPATWSYYKEGWHHFAFVYNASSGFKAMYIDGVCPSGFSKTLP
                     GGTITVPSDQTIYIGSNQTFQSGTIAFDEIAVYNQAISANEIYGNYLATQSGQHYTFT
                     SSTPPTPPAVTAGLDINEFPLGYVLGSTNSNSVSTPALTQLKNFPRPRYRIETTAPPN
                     FNWMAIDYMSGYYQPGVNAQMMCDTAGAMSIELSENWNYYFLVNPNTNSISYSDTNTI
                     EGKLVRVSNRNRQYKTSAISFWVQQNPSRYGYQANGEYMTWQNCPANNFIRNSSGQFV
                     GSDGAVSTTKYRAPSSPLDSIKLDASTYRRNLSALAAVMTDTLNLINDNDEILTLFDS
                     TVLRADPAILAAMGTAGFGNMRQYAAYGYQNMFKVLKDSVRTNISAFSGVGFSMYSVC
                     GWDGSISQVPYWRWNYSQYKKCNEDPYFGNTSTFDFYPRYPYNWRKNQGPWLGLQPWI
                     ESRNVEQASLTKYTTPFVGAGWNVNEEQNIRPAQWLGLLKVLGMMGARSYYAGYFNEA
                     SSYVPPTPPPADPKGYIWQAAMPSYAQASCDWVMDNAYTDTLMAGDVPNNYQFPTCNG
                     YRFYSGSEEIWTVVRKKNTSNQYAIATAYIPSTSMIGGTPLSDTAVFTLQSKSISLET
                     RRQGSVYCVDLNKDTAVVVQYDDWHEYKHPQRWSQDMYVQAEHYGAYVSGQKDVRTIP
                     YQSSSSSSLNFVGTTTYLTWRDSTTYTRDTLSYQVNVPSSGTYNLWVRMRSLNGTTAG
                     FSARLDNGTAFTQSNVIDTSFLWYRISGTPDTIRWSGVTAGRHTVKIFPTSARTEIDQ
                     FLFTSSTTVVLPEGTPGSSTPCGVAFTPTITPSGPVTQCGGSVSLQASAGNAYVWSNG
                     QTSQSISVTTSGSYRVTVTDGAGCTGTSAVVVVTINTPASAGVTPSSGVYCGTNITLS
                     SSSATSYLWSTGATTQTISVGAGSYTVTVTNASGCTASASSTISSGTVISATISPSGT
                     VTQCGGTATLTSSPASSYLWSNGLTSQSITATAGVYTVTVTSSTGCTATSSATTVVIN
                     PPVSAAISPSSAIYCGSNVTLTASAGASYLWSNGAVTQSVSVGAGTYTVTVTSAAGCT
                     GTASRTVSSGTAATPTISPSGTVIQCGGTVTLTSSAGTSYLWSNGSTAQSVVVGTGSY
                     TVRVTNSSGCSAVSAPTYVTINANPTASITPSGTLTLCTGTTQTLTAGNATSYAWSTG
                     ATTQSINVSTGGSYYVTINNSTGCSSTSAPVVVSYYDCNCQAPTGAIITNIWKASARV
                     QWTYNAQATRYRVTVTQVTDPTKTQTKYWRGGDTVISFVNLRSGTQYSVTIVPVCGGT
                     TGSSTTLYFVTKP"
     gene            4204..4503
                     /locus_tag="PLE23_07985"
     CDS             4204..4503
                     /locus_tag="PLE23_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83240.1"
                     /translation="MQIRLSNIINKNCYCIEVMGHIPNALNADIPIVRVYLDEKQSRE
                     WDQRIKLIKDKSPIYEGLEVIEDESGDKIIREKFYAKAKELLHSMGEPREDYYWP"
     gene            4542..4615
                     /locus_tag="PLE23_07990"
     tRNA            4542..4615
                     /locus_tag="PLE23_07990"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4575..4577,aa:Met,seq:cat)
     gene            4764..4837
                     /locus_tag="PLE23_07995"
     tRNA            4764..4837
                     /locus_tag="PLE23_07995"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4797..4799,aa:Met,seq:cat)
     gene            complement(5206..>6814)
                     /locus_tag="PLE23_08000"
     CDS             complement(5206..>6814)
                     /locus_tag="PLE23_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83241.1"
                     /translation="FLTMAELKDMGASEDDMKAAALAYQNVWNNPGFNQVWYNNSWNY
                     QLMSLYKVAILDMDFSSWNTEYYEFRALASTGQEKGFRVDEKEYGSRKNRKYNQKNYE
                     RRYQGKWIIGTNIMLPGYGYQYDQVFDYESRPKCSYSVYRTGQRSVTSRCVSTIDDMQ
                     LAVLKFRNAWAKAAPAGILIEWGSLTEMVHAGRKLKPLDMIKMWRNTGDLLYKGPKTP
                     DGRTLQGTNPPIMPLTNGIGNMMAEFADSWNIYNQTLAELTAIGRGQDGTMPAPDTLV
                     GVANIAEAATQDTFRPIIMGYKRLKSRVNNNVALRWQLRSCEGDVDEYVEKREGAAGE
                     IVRLSYDDIRGRIIQINCESIIDDSQKQQVLVAADRSLQAAKAGQVGISMADFLIIMQ
                     AIERGQVKYALMWLHYREEQMKAEQIQREQMNQQANTEGAIALKQADQQNLQAELAMK
                     KQLSAQEGANKIQEIQEKGKQDRETLLLKFQLERGVMPSSLGVPSLPTLSDSPAPSPV
                     VEPAPAATPSEPMPSQPQAQALSEIGL"
BASE COUNT         1713 a         1743 c         1682 g         1676 t
ORIGIN      
        1 gtaggtccgg ccacatggtc ttactacaag gaagggtggc accactttgc ctttgtctat
       61 aatgcaagct cgggcttcaa ggccatgtat attgacgggg tgtgtccatc gggattcagt
      121 aagacactgc ccggcgggac catcaccgta ccttccgatc agaccatcta catcggaagc
      181 aatcagacct ttcaatccgg taccatagcc ttcgatgaaa tcgctgttta caatcaggcc
      241 atcagcgcca atgagatata tggcaactat cttgctacgc aaagcggtca gcactatacc
      301 ttcacctcat ctacaccgcc cactccgcct gcggtcacgg caggtcttga cattaacgag
      361 ttcccattgg gctatgtgct tggtagtacg aactcaaact cggtaagcac tccggcgctc
      421 acgcagctca agaattttcc gcgaccacgc taccggattg aaaccacggc gcctccaaac
      481 ttcaactgga tggcgattga ttacatgtcg gggtattatc agccgggcgt caatgctcaa
      541 atgatgtgcg atacggccgg agccatgagt atagagctta gtgaaaactg gaactactac
      601 ttccttgtta atccaaatac taactcgata agttattctg ataccaatac aattgaggga
      661 aaactcgtaa gggtaagcaa ccggaaccgg cagtataaaa catcggccat ctcgttctgg
      721 gtacagcaaa acccgagtcg ctacggatat caagccaatg gagagtacat gacatggcaa
      781 aactgtccgg caaacaactt tataagaaat tcatcagggc agtttgtagg ttccgacgga
      841 gcggtgtcaa ctacaaaata cagagcgcct tcttcgcctc tggacagcat aaaactcgat
      901 gcaagtacat accgaagaaa cctcagcgca ttggccgctg tgatgaccga cacccttaat
      961 ctgatcaacg ataacgatga gatattgaca ctgtttgact ctacggtatt gcgtgccgac
     1021 cccgctatac tggccgctat gggaacggcc ggattcggga acatgaggca gtatgcagcc
     1081 tatggatatc agaacatgtt caaggtactc aaggatagcg tgcgcacaaa catcagcgcg
     1141 ttctcgggtg tagggttcag catgtattcg gtatgcggat gggacggaag catctcgcag
     1201 gtgccgtatt ggagatggaa ctactctcag tacaagaagt gcaatgaaga cccgtacttt
     1261 ggaaacacat caacctttga cttctacccc cgctatcctt acaactggcg caagaatcaa
     1321 ggcccatggc tcggtcttca gccgtggata gaatcaagga atgtagagca ggcgagcctt
     1381 acaaaataca ccactccctt tgtgggtgcc ggatggaatg tgaatgaaga gcaaaacata
     1441 cgtcctgcgc aatggcttgg attgttaaaa gtgcttggca tgatgggcgc aaggtcttat
     1501 tatgccgggt attttaacga agcctcaagt tatgtaccac ctacccctcc gcctgccgat
     1561 cccaaggggt acatctggca ggcggcaatg ccgagttatg cacaggcttc ttgtgattgg
     1621 gtgatggata atgcctacac cgacaccctg atggctggtg atgtacccaa caactatcag
     1681 tttccgacat gcaatgggta ccggttctac tcaggaagtg aggagatatg gacggtggtt
     1741 cgtaaaaaga atacaagcaa ccagtatgct attgctacgg cttacatccc ctctacctct
     1801 atgattggcg gtactccact tagcgatacg gcggtattta cattacagag taaatccatt
     1861 agccttgaaa caagacggca gggatcagtc tattgcgtgg atctgaataa agacactgcg
     1921 gtagttgtac agtacgatga ctggcacgag tacaagcatc ctcagagatg gagtcaggat
     1981 atgtatgtgc aggccgaaca ttacggagcc tatgtaagcg ggcagaagga cgtgcgtact
     2041 attccatacc aaagctcttc gtcatcgtcc ttaaattttg taggcaccac gacttatctt
     2101 acttggcgcg actccaccac atacactcgt gacaccttga gttatcaggt gaacgtcccg
     2161 tcttcgggca cctacaatct atgggtgcgt atgcgaagcc tgaatggtac tactgctggc
     2221 ttctcggcac gccttgacaa cggcacagcg tttacgcaga gtaatgtgat cgacacttcg
     2281 ttcttgtggt atcgtatctc cggcactccc gatacaatca ggtggtcggg agtcactgcc
     2341 ggtcgccata ccgttaagat attcccgaca tcggcacgaa ccgagattga ccagttcttg
     2401 tttacttcgt caacaacggt ggtgttacct gaaggaaccc cggggtcttc gactccatgc
     2461 ggagtagcct tcactccaac catcactcca tctggtccgg ttactcagtg cggtggtagc
     2521 gtatcacttc aggcgagtgc gggtaacgcc tatgtatgga gtaacggaca aacctcgcag
     2581 agtatcagtg tgaccacatc ggggtcgtac agagttaccg ttaccgatgg agccggatgc
     2641 accggaacat cggcggtggt ggtggtaacc atcaatactc cggcaagtgc aggggtgacg
     2701 ccatcaagcg gagtgtactg cggtaccaac atcaccctat cttcgtcctc ggctacttca
     2761 tatctgtgga gtacgggcgc gaccacgcaa accatcagcg taggggctgg aagttatact
     2821 gtcacggtaa ccaacgcatc aggatgtacg gcaagcgcgt cctcaacgat ctccagcgga
     2881 actgtgatat cggcgaccat cagtccatca ggtacggtca cgcagtgcgg tggtacagca
     2941 actctcacca gtagtcctgc aagctcttat ctctggagta acggactgac ttcacaaagc
     3001 attacagcca cagcgggggt atatactgtc acggtgacct cttcgacagg atgcacggcc
     3061 acctcatctg caacaacggt ggtgatcaat cctccggtaa gtgcggctat cagtccatct
     3121 tcggcgatat actgcggatc aaacgtaacg ctcacagcca gtgcgggggc atcctacctg
     3181 tggagtaatg gcgcggtaac gcagagtgtt tcagtaggcg caggcaccta taccgtcacg
     3241 gtgacaagcg cagccggatg cacgggaaca gcgagccgga ctgtctcaag cggtactgcc
     3301 gcaacgccca ccatcagtcc gagtggtacg gtaattcaat gcggaggtac tgttacgctg
     3361 acttcaagtg ccggaacctc gtatctgtgg agcaacggat ctaccgcgca gagtgttgtt
     3421 gtgggtaccg gtagctacac agtaagggtg actaactcat caggatgctc ggcggtgtct
     3481 gccccgacat acgtgaccat taatgccaac ccaacggcaa gcatcactcc atctggtaca
     3541 ctaacgttat gcacaggcac cacacagact cttacggcag gcaacgccac ttcttacgca
     3601 tggagtacgg gtgctaccac gcagagtatt aacgtgtcaa cagggggttc gtattacgtc
     3661 accatcaata atagcacggg atgtagttca acttcagcgc cggtggtggt atcgtattac
     3721 gactgcaact gtcaggcgcc gactggagct atcataacga atatatggaa ggcgtccgcc
     3781 cgggttcaat ggacatataa cgcgcaggct acacgataca gagttactgt tacgcaggtc
     3841 acagacccta caaagacaca gactaaatac tggaggggtg gtgacactgt gatttctttt
     3901 gtaaatctga ggtctggcac acagtattct gttacaatag ttccggtatg cggaggcacc
     3961 acgggtagct ctaccacact atacttcgtc acgaagcctt gatgttgaga tcaagacccg
     4021 tcagaaatgg cggggttttt attgtaattt tacaataatc aagcatacaa gcgagataaa
     4081 tatttgcttt tcatttatat acacaaatca ataaaaattg taaatttaca ttgcacccca
     4141 ctcccgatag catcgtgagg ccgacccctt gcagaaatgc agggggtttc gtatgtttgc
     4201 gatatgcaaa taaggcttag caacattatt aataaaaact gttattgcat tgaagttatg
     4261 gggcatatcc ccaatgccct taatgcagac atccctatcg taagagtgta tcttgacgaa
     4321 aaacagtcga gagagtggga tcaacggata aaactaatca aagacaaaag cccaatttat
     4381 gaaggcttag aggttataga agatgagtcc ggtgataaga taatccgcga aaagttttac
     4441 gcaaaggcaa aggaattatt gcactctatg ggagagccac gcgaagatta ttattggccg
     4501 tagccccttg cagaaatgca gggggtttcg tatgtttgca ctgcgaggtg gagaagtggt
     4561 catctcgtta gcctcataag ctaaagatcg caggttcgaa tcctgcccta cgcaacaacc
     4621 taagtggatg cctcaccgaa aaagtgcaat tattgtatgt acagcgatgg ttgcagtttc
     4681 ttgtttgctg acgagtagtc aagaataggt gaggccagag attgacccct tgcagaaatg
     4741 cagggggttt cgtatgtttg cactgcgagg tggagaagtg gtcatctcgt tagcctcata
     4801 agctaaagat cgcaggttcg aatcctgccc tacgcaacga aataccggca aaggctctca
     4861 cagcgttgaa tggtatccac caccccgccc accagcgggg tttgttgttt ataggcttcc
     4921 gattaatgca ctcgatgaca gatcagcaaa ttgccgcctt gcttattgat tcatattcct
     4981 tgcttaggtg cggggtttat gttcacgaca taaatgtcgt gaacatatcg tatcttcgtg
     5041 ctataaacac aaccaccatg caaaaactcc gcatcaaaca aattcagacg tttcgctctg
     5101 agaaagacaa tcagttctat atgcgtgtta taggactcaa ctcacgcacg gtccataccg
     5161 ctgaagggtt taagacccga aggggacgcg acaactccgt gcagattata aaccaatctc
     5221 tgataaagcc tgtgcctgtg gttgagatgg catgggttca gaaggggtcg cagcgggtgc
     5281 tggctccaca accggcgagg gcgctggtga atcgctcaaa gtcggcaaag aaggcacccc
     5341 taaagaagaa ggcataacgc ctctctctaa ttgaaatttc agaagcaggg tctcacgatc
     5401 ctgctttcct ttttcctgta tctcctgtat cttattagca ccctcttgcg ctgacaactg
     5461 cttcttcatg gccagttccg cttgtaagtt ttgctgatcg gcctgcttga gtgcgattgc
     5521 cccttcggta tttgcctgtt gattcatctg ctcacgctgg atctgttcgg ccttcatctg
     5581 ctcttctctg taatgcagcc acatcagggc gtacttcacc tgtccgcgct caatagcctg
     5641 catgatgatc agaaagtcag ccatactgat acccacctgt ccggcttttg ccgcctgaag
     5701 gcttctatca gcggcgacaa gcacctgctg tttctggctg tcatcgatta tgctctcgca
     5761 gttgatctgt atgatacgac ctcggatgtc atcgtaactc aaacgcacga tctctccggc
     5821 ggcaccctct cttttctcca cgtattcatc cacatcgcct tcgcaggatc gcaactgcca
     5881 gcgcaaggct acgttgttgt tcacccttga cttcaggcgc ttgtagccca tgataatagg
     5941 tctgaaggtg tcttgcgtag cagcctctgc gatattagcc accccaacaa gagtgtccgg
     6001 tgccggcata gtgccgtcct gtcctcttcc aatagcggtg agttcagcaa gtgtctggtt
     6061 gtagatgttc cagctatcgg cgaactcggc catcatattc cctatgccgt tagtcagtgg
     6121 catgatcgga gggttggtac cctgaagtgt gcggccatcg ggagtctttg gtcctttgta
     6181 aagcaggtca ccggtatttc tccacatctt gatcatatca aggggtttta gctttcgtcc
     6241 ggcatgcacc atctcagtaa ggctacccca ttctataagg ataccggcag gggcggcctt
     6301 cgcccatgcg ttacggaact tcagtacggc aagctgcatg tcatcaatgg tgctcacgca
     6361 tcttgaagtc actgatcgtt gtccggtccg gtacacgctg tatgaacact ttggacggct
     6421 ctcgtagtcg aacacctgat cgtattggta tccgtagcct ggcagcatga tattggtacc
     6481 aataatccac ttaccctgat atcgacgctc gtaattcttc tggttgtact tccggttctt
     6541 acggctacca tactccttct catcaaccct gaatcccttc tcctgtccgg tagaggcaag
     6601 cgctctgaac tcgtagtact ccgtgttcca tgaactgaag tccatatcca gaatcgctac
     6661 cttgtacagg ctcatgagct ggtagttcca tgagttgttg taccatacct ggttgaagcc
     6721 cgggttattc cacacgttct gatacgccaa cgcggcagcc ttcatgtcgt cttcgctcgc
     6781 gcccatgtct ttgagttcgg ccatagtcag gaac
//
LOCUS       DAOQQY010000150         6805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4688_length_6805_cov_6.612444, whole genome shotgun sequence.
ACCESSION   DAOQQY010000150 DAOQQY010000000
VERSION     DAOQQY010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4688_length_6805_cov_6.612444"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2071)
                     /locus_tag="PLE23_08005"
     CDS             complement(<1..2071)
                     /locus_tag="PLE23_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83242.1"
                     /translation="MNTFKQTKHWVIIILLIAFNHFVYAEGSVDFLKYPGKRMFYNAE
                     QTQQLKVFAKSGEFINFGASHVGITEGFIQVYTPDGKLYAVYDNTGATEGFAIINNNI
                     EEFNGPTGGGSLNGNGYKPGIVAVESANEGVWTFVLGFPKYSKLTFPNLNNTDPWTRV
                     KDQPTIQRCILAWDITVSKIKAANEGGEMLKGRVYSNEYISIINQNGNYSSPKFFILS
                     PAGFIYRVDLNDTDPWGFPISSNNIGLTDAAQKSIYKSLNQTDYTRSADPTSWTPGQN
                     YFYDPQAKDLGNFINNKVFINLPSDDMPANALVTDVFRNETYATWLNNSPQIAGPKID
                     NLLFKPGNPTGNGCPPGLYKEGDGGVFSFDVNLPGSVSLQLDLNNDGDFNDAEDKVYF
                     QSVKKGNNSLYWNGTFGNGAVIPAQSNYTFNFKLTFRTGEIHIMMADIENNPGGVNIT
                     RINGPNSPANSFYYDHTAIGGPVSGGGTPSNTLPTSIPFTYSSNFGNEKMLDYWSYIE
                     DTKTGAASFTIDKECNDKYPDTDGDGIPDNIDLDDDNDGIADYLEFCNPDSSFVCLPG
                     GLDPSGDADGDFIPNYLDANDPAVANNCPDINGDGICDKLSEIYDTDGDGVPNHLDLD
                     SDNDGISDLIESGYDVPDANGDGRIDFSTGAFGTNGFYVPLSTDANSLTAKAKTKPAD
                     TDKDGKYD"
     gene            complement(2242..3489)
                     /locus_tag="PLE23_08010"
     CDS             complement(2242..3489)
                     /locus_tag="PLE23_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX83243.1"
                     /translation="MRVAIYEGIRQAVGQLIANKLRSFLSLLGITIGIFCIISVKSAV
                     DSLEDNIRDSFRKLGTDVLFLSKMPWNEDPGENYWKYLRRPNPSYKDYEFINKHVKTA
                     EMVSFTVFAGSKTAKFQSNSVENCFIIGCTQEYPSMFNLQLSEGRHFNNLEYRNGIYC
                     CVIGATIAENLFPGIDPIGRTITLAGYKAYVTGVVKKSGNDIINPMNFDEVIMIPYTM
                     MKKIFNTKPEGPFSNTNVNIKARKGVDIEILKDDVIAALRARHQLQPKEANDFSLNEI
                     SLLDSVIKVFFGVLNIAGFVIGGFSILVGMFSVANIMFVSVKERTNIIGIKKALGARQ
                     NIILLEFLTESVLLCILGGILGLAVVLIATLVMSKVLEFDIYLSANNIITGIGLSVVI
                     GILAGFIPALQAARMDPVEAIRK"
     gene            3987..5948
                     /locus_tag="PLE23_08015"
     CDS             3987..5948
                     /locus_tag="PLE23_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HMX83244.1"
                     /translation="MSKPQHNSLNKVSFAGILVTLGIVYGDIGTSPLYALKAIVSDKV
                     ITPDLVLGGVSLVFWTLALITSFKYVRLALDIDNNGEGGIFALFAMVRRKNRGWLIFL
                     AIIGCSTLMADGFLTPSISISSAVEGLILLNPNIKTVPIVIGILIVLFVFQQFGTNVV
                     GKTFGPVMTLWFLTMAIFGFLALIEYPQVLKAINPYYGIHLLANYPKGFLILGGVFLC
                     TTGAEALYSDLGHCGKKNIRVSWIFVNISLILCYFGQGAELLKNNMGEVWPADRSVFY
                     QIAPNGLLPLTIIIASLAAIIASQALITGAFSLINEAMKLRLIPLLKINYPTQLRGQI
                     YIPFINWLLLGGCILVVLIFRESTNMEAAYGLAITINMLMTTTLMVIFYYYHKVKLLY
                     IIVFGAVFYTIEGAFFLSNIDKFFHGGWFSFLISAFFVFVMYIQLRARALRTKHIAFV
                     NVNEYKEKLIALMGDETIPKEATNLVFMSMSGNKEMIDSNIIYSIFRKKPKRADVYWF
                     VHMEILNEPFERNYSVDTIIPQKCFFVTVRYGFKVEHKINLAMNQIMNDMVERGEVDN
                     ITRHPSLRQFNMPGDFKYYLINSRVSVDDRITPVEQFIIRVYRIIKSISLPPDKDFGL
                     EVSNVVVETVPINIGLRQTKTIDGLHRVK"
     gene            5955..>6805
                     /locus_tag="PLE23_08020"
     CDS             5955..>6805
                     /locus_tag="PLE23_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HMX83245.1"
                     /translation="MKQRLKDKFGNRFAPFTLGILLVVSILHLGVWQGWFSLPDEVLM
                     VSRYAVLTLLCLYAFLKESLTTWILLSMLIGAEIGHDFPALAVNLKILSKVFLRLIKT
                     IIAPLLFGTLVVGIAGHSNLKQVGRMGWKSIVYFEIVTTIALVIGLIAINVSKAGVGI
                     NLPPTEHGEKLPEVAQQTTEDVILHVFPENIAKSVAEGQILQIVIFSILFGIGLAQLN
                     DKHKKPMLDFAESLSEVMFNFTKIIMKFAPIGVGAAIAYTVGHMGLGILVNLFKLLAT
                     LYVALIV"
BASE COUNT         1925 a         1315 c         1389 g         2176 t
ORIGIN      
        1 catcgtattt tccgtctttg tctgtatctg caggttttgt ttttgccttt gcagttaaag
       61 aattagcatc agtagaaaga ggaacataga acccatttgt gccaaatgcc cctgtactaa
      121 aatcaattct accatcacca ttggcatcag gcacatcata tccagactct atgaggtctg
      181 atattccatc attgtccgaa tcaaggtcga ggtgatttgg aactccatcg ccatcggtgt
      241 catatatttc agaaagttta tcgcagatgc cgtctccgtt aatgtcagga caattattgg
      301 ccactgccgg atcattagca tctaagtagt ttggtataaa gtcaccgtca gcatcaccgc
      361 ttgggtcaag tccaccaggt aggcacacga aactgctgtc cggattacag aactcaagat
      421 agtcagcgat gccgtcatta tcatcatcca aatctatatt gtctggtatg ccatcaccgt
      481 ctgtgtcggg gtatttgtca ttacattctt tatctatggt aaaagaagca gcacctgttt
      541 ttgtatcttc gatataagac cagtagtcga gcattttttc gtttccgaaa ttggaactat
      601 acgtaaaagg aatgctcgtg ggaagtgtgt tggaaggtgt gccaccacca gaaactggac
      661 caccaatggc agtatggtcg taatagaaag aattggccgg actgttaggg ccattgatac
      721 gagtaatatt gacaccaccc ggattattct caatatctgc catcatgatg tggatttctc
      781 ctgttctgaa tgtcaatttg aaattgaatg tgtaattgct ctgagctggt attacagcgc
      841 cgtttccgaa agtgccgttc caatagaggc tgttgttgcc tttttttact gattgaaaat
      901 aaaccttgtc ttctgcgtca ttaaaatcac catcgttgtt taaatcaagt tgcaatgaaa
      961 ccgaaccagg taggtttacg tcaaaactga atacacctcc gtcaccttcc ttatacagac
     1021 ccggcggaca accgttaccg gtcggatttc ctggtttgaa caatagattg tcaatttttg
     1081 gtcctgctat ctgcggcgaa ttgttgagcc aggtagcata ggtctcattc ctgaaaacat
     1141 ctgttacaag cgcatttgcc ggcatgtcat cacttgggag attgataaat actttgttgt
     1201 tgatgaaatt acccagatct ttagcctgtg ggtcatagaa ataattctgg ccaggggtcc
     1261 agcttgttgg atcagcagac cgtgtatagt ctgtttgatt caatgatttg taaatagatt
     1321 tctgtgcagc atctgtgagt ccgatgttgt tagaactgat tgggaaaccc catgggtcag
     1381 tatcatttaa atcgaccctg taaataaatc ccgccggact aagaatgaag aacttaggcg
     1441 aagaatagtt gccattctga ttgatgattg agatatattc gtttgaatac acacgtcctt
     1501 tgagcatttc gcccccttca tttgccgcct ttattttcga tacggtaata tcccatgcca
     1561 aaatacatcg ctggatggta ggctggtcct ttactctggt ccaaggatcg gtattgttca
     1621 ggttggggaa agtaagtttt gagtattttg ggaaacccag cacaaaagtc cagacacctt
     1681 cattcgctga ttcgactgct actattcctg gtttgtaccc gttgccatta agcgaaccac
     1741 cacccgtagg gccattgaac tcttcaatgt tgttgttgat aatggcaaag ccttcggtcg
     1801 cgcctgtatt gtcatacact gcatacagtt tgccgtcagg agtatatacc tggataaaac
     1861 cttcggtaat gcctacatgg cttgcaccaa agttgataaa ttcgccgctt ttcgcaaaaa
     1921 ccttaagctg ctgtgtttgc tctgcattat aaaacatcct tttgccagga tattttagaa
     1981 aatcaacaga accctcagca tatacaaaat gattgaatgc tatgagtaaa attataatta
     2041 cccagtgttt tgtttgttta aatgtattca tcggctcaga ttaaaacaat taatttgcag
     2101 tattcgtgtt gaatactgcc tgcaatctga atgtttaata caaatattga accaatttgt
     2161 atttgttaaa attaaatatg attttttgac tgttaaattg taggtaacaa tattgaaaaa
     2221 cctgtaaaag tactgtcgtt tttatttcct tatggcttct acggggtcca ttctcgccgc
     2281 ctgtagagcc ggtataaatc cggccaaaat accgatgaca acagatagtc cgataccggt
     2341 aataatattg ttggcagaaa gataaatatc aaattccagc acttttgaca taacaagggt
     2401 agcaatgagt acaactgcta gcccaagtat gcctccgagg atgcacagca aaacagactc
     2461 tgtcaggaac tccagcaaaa tgatattttg tcttgctccg agtgcttttt taatgccgat
     2521 gatgttggtt cgttctttaa cagacacaaa cataatattg gcaacgctaa acattccaac
     2581 cagaatggaa aatccgccaa tgacaaagcc ggcgatgtta agtacgccaa aaaatacttt
     2641 aataacagaa tcgagcaaac ttatttcgtt cagcgaaaaa tcgtttgctt cttttggttg
     2701 tagttgatgc cgagctcgta gtgctgctat cacatcgtcc ttgagaattt ctatatcaac
     2761 tcctttcctt gctttgatgt taacatttgt attcgaaaac gggccttcgg gcttggtgtt
     2821 gaatattttt ttcatcatcg tataaggaat catgatgact tcatcaaaat tcatcggatt
     2881 gataatatca tttccggact ttttaaccac gccggttaca taagccttgt agccagccaa
     2941 ggtgatggta cggcctattg ggtctatacc ggggaaaagg ttttctgcaa tggtagcacc
     3001 gatgacacag caataaatgc cattgcggta ttcgagatta ttgaaatgcc ttccttccga
     3061 caattggaga ttaaacatag aagggtattc ctgggtacag cctatgatga aacagttttc
     3121 gaccgaatta ctttgaaatt tcgctgtttt ggagccggca aatacagtaa aggaaaccat
     3181 ctcggctgtc tttacatgct tgttgataaa ctcatagtct ttgtagcttg ggttaggccg
     3241 cctcaggtat ttccagtaat tctcgcccgg gtcttcattc catggcattt tagaaaggaa
     3301 taatacgtct gttccgagtt ttcgaaagct gtctcttata ttgtcctcaa gcgagtcaac
     3361 ggctgatttt acagaaatga tacaaaaaat accaatggta atgccaagaa gtgacagaaa
     3421 acttctgagt ttgttggcaa tcagctggcc tacagcttgc ctaattcctt catagatggc
     3481 taccctcata tttttatact tactgcgaaa ttaagcaggt gctgtaacac ttgttgatta
     3541 tttcgatgaa accaaacatt tattctgcaa aatttttttt aattatactc aaatcacaag
     3601 taggtatttc tgtagtaatg gtacattgcc ggtaaaaaat aattttgtat ttacacgaag
     3661 tataatataa atttgcccat tcttttataa caaaacaaac aacctgactg cctgtagata
     3721 ggtattcaag tgattatgaa ctatttaaag ctaaaaattg tcaactgttt tttttgaacg
     3781 ggcaactcat ggtgttgaaa tagatgaatg cgtgaaccac cgtattcatg agtttcgagc
     3841 aattaatgct tgctgccagc tttcaagttt tcaaaagaac tcaagtaata tccatttact
     3901 tacatttttc gattctgtat atttgaaaaa tttctgcaat gtctcacagg tttgtagaaa
     3961 atattttatg ccattaattt acatttatgt caaagccaca gcacaatagt ctcaataaag
     4021 tcagttttgc aggtatcctt gttacgctcg gtattgtata tggcgacatc ggaacatcgc
     4081 ctctttatgc attaaaagca atcgtatcag ataaagtaat cactcccgac cttgtactcg
     4141 gaggtgtctc gcttgttttc tggacacttg ctttgattac ttcgttcaag tatgtgcggc
     4201 ttgctctcga cattgacaat aatggtgaag gaggaatatt tgctttgttt gcaatggtaa
     4261 ggcggaagaa tcgcggatgg ttaatctttc tggctatcat tggttgttca acgctcatgg
     4321 cagatgggtt tttaacacct tccatttcta tttcatccgc tgtggaaggt ttaatactgt
     4381 taaatccgaa tatcaagacc gtacctatcg ttattggtat tttgattgtt ctgtttgttt
     4441 ttcagcagtt tggtaccaat gtagtaggca aaactttcgg tccggttatg accttatggt
     4501 ttttaactat ggccatcttt gggtttttag cattgataga atatccgcaa gtactgaagg
     4561 caatcaatcc ttactacggt attcacttgc ttgccaatta tccgaaaggg ttcttgatac
     4621 tgggcggagt atttctttgt acgacaggtg ctgaagcttt gtattcagac cttggtcatt
     4681 gtggaaagaa gaatatcaga gtaagttgga tttttgtaaa tatttctctc atactttgtt
     4741 acttcggtca aggagctgaa ctgcttaaaa acaatatggg tgaagtctgg cctgcagatc
     4801 gttctgtgtt ttatcaaata gcaccaaacg gattattgcc acttactata ataattgcat
     4861 ctctggcagc cattatagca tctcaggcat tgattacggg agcattttca ctgataaatg
     4921 aagccatgaa gctcaggttg atccctttgc tgaaaataaa ttacccaacc cagctgcgag
     4981 gccagatata catacctttt attaattggt tgttgttggg tgggtgtatt ctggtggttt
     5041 tgatatttag ggaatcgact aatatggaag ctgcatacgg cttggccatt acgattaaca
     5101 tgcttatgac aacgactctg atggtaatct tttattatta ccacaaggtt aaattactct
     5161 atattattgt ctttggggca gtgttctata ccatagaagg agctttcttt ttatcaaata
     5221 ttgataagtt tttccatggg ggatggttca gtttcttaat atctgctttt tttgtgtttg
     5281 tcatgtatat acaattgcgt gcaagggcat tgcgtacaaa acatatagca ttcgtaaatg
     5341 tcaacgaata caaagaaaaa ctcattgctt tgatgggaga cgaaacaata cccaaggaag
     5401 ctaccaacct ggtatttatg tccatgtccg gtaataaaga aatgatcgac agtaatatta
     5461 tttattcgat tttcaggaaa aaacctaaaa gagcagatgt ttactggttc gtacacatgg
     5521 aaatactgaa tgagccgttt gaaagaaatt attcggtaga taccatcata cctcaaaaat
     5581 gcttctttgt cacagtacgc tatggcttca aggtcgaaca caagattaac ctggccatga
     5641 accagattat gaatgatatg gtcgaacggg gagaagttga caacatcaca cgacatcctt
     5701 cgctacggca atttaacatg ccgggcgact tcaaatatta cttgattaac tcaagagtat
     5761 cggttgatga tagaataact ccggtagaac aattcatcat tcgggtgtac agaataataa
     5821 aatctatatc tttaccgcca gacaaagatt tcggcctaga agtatcgaac gtagtagtcg
     5881 aaacagtacc gatcaatatc ggactacgac agacaaaaac aattgatggg ctgcatcggg
     5941 taaaataatt ttttatgaag caaagattaa aagacaaatt tgggaacaga tttgccccat
     6001 ttaccttagg catacttttg gtagtaagca tcttgcacct tggtgtatgg cagggttggt
     6061 tttctctgcc tgatgaagtt ttgatggttt cacgttatgc tgttcttacc ttgctttgtc
     6121 tgtatgcgtt tttgaaagaa tctcttacta cctggatact gctgagtatg cttatcggtg
     6181 ctgagattgg tcatgatttt ccggctctgg cggtcaatct gaagatactg agtaaggtgt
     6241 ttcttcgcct gattaaaacc attatcgctc cattgttgtt cggtacactc gtggtaggca
     6301 ttgccgggca ttctaacctg aagcaggtgg gcagaatggg ttggaaatct attgtatatt
     6361 ttgaaattgt caccaccatt gcactggtca ttggccttat agctatcaac gtatcaaaag
     6421 ccggtgttgg tatcaatctc cccccaactg aacatggaga aaaactaccg gaagtagcac
     6481 aacaaaccac cgaggatgta atacttcacg tttttccgga gaacattgct aaatctgtag
     6541 ccgagggtca gatattacaa atcgttattt ttagtatttt attcggtatc ggtcttgcac
     6601 agctgaatga caaacataaa aagccaatgc tcgattttgc tgaatcattg tctgaagtaa
     6661 tgttcaattt tacaaagatt atcatgaagt ttgccccgat tggtgtcggt gcagccattg
     6721 cttatacagt ggggcacatg gggctgggca ttcttgttaa cttattcaaa ttactggcaa
     6781 ctctgtatgt ggcattaatt gtttt
//
LOCUS       DAOQQY010000151         6650 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4870_length_6650_cov_5.788779, whole genome shotgun sequence.
ACCESSION   DAOQQY010000151 DAOQQY010000000
VERSION     DAOQQY010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4870_length_6650_cov_5.788779"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="PLE23_08025"
     CDS             <1..915
                     /locus_tag="PLE23_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83246.1"
                     /translation="GDGYSHNLYINHIAEFTFRFNYTHDSKIGHLLKSRAHKTYILYN
                     RFDSPKNGNPSREIDLPNGGLAIFIGNIIRQTQNAENSNLVGYGLEGLTNTAPHDIIM
                     INNTLINEKANGSFVHVQNGTNLLKMWNNILAGGGSTINQSPTILDTMSNYYTPNISD
                     ASFVDENNQDYHLTTGSWAANHGADPGYYQDYQLLALYEYDEVLGKIDRKFIDKIDIG
                     AFESDISTTIQETKQSHSLNIKGNCITNTGIAGLAMKISAVTGSDIYSGLIGAGEQKC
                     FENLVPGIYIITARTKDRLVASRKFLIN"
     gene            923..1309
                     /locus_tag="PLE23_08030"
     CDS             923..1309
                     /locus_tag="PLE23_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83247.1"
                     /translation="MEQQYYHLDSFELFGKKFYRIIILPDETVFIELNKTVKGCGSKP
                     DGFFWKKVMEYGAIKWAVEELENFDFDAEKEAAIVCHESKDTLLQLCSKTFEMLENPA
                     LFKSIISEIQAISLRSDEDDLLDFQE"
     gene            complement(1269..2156)
                     /locus_tag="PLE23_08035"
     CDS             complement(1269..2156)
                     /locus_tag="PLE23_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMX83248.1"
                     /translation="MKLSYGVKQMLLSTFYFAVMNVLIKMLTDIPPMEIVFFRCLLAA
                     IFCFGLLARKKVSWLGHNRKLLLLRGFFGTLALFTYFITIKALPLGTAVTIQYISPVF
                     TSIIGIFLVKESVKPLQWLFFAVSFAGVLIIKGFDFTISTQMLTIGLISSVASAFAYN
                     YVRSMKESEDPIVVVLHFQLFGVVAGLVAAMFQWQMPSGNQWLILLAIGILTQLGQTS
                     LTLALQEERLADVSILNYLGVLYALGFGILFFGEMYGISTIVGILFIIGGVLANYYYK
                     KRYEQSFIPENQADHLHQT"
     gene            2255..2923
                     /locus_tag="PLE23_08040"
     CDS             2255..2923
                     /locus_tag="PLE23_08040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMX83249.1"
                     /translation="MQQGTPNIIFEDNHIIIVDKPHGWLVHGDKTNDQTIGDWLKQYI
                     KQKYNKPGDVFLGTVHRLDRPVGGLMVFARTSKSLERLNKSFADKHVKKYYQALVQNC
                     PEKLSAYLVHWLRKEEVRNSVELRNDAAGQEWKKAETNYQVLGKYNQYFLIALEPITG
                     RKHQLRAQMASIGSPIVGDLKYGSKIRCSDGNIALKCTGLEFIHPVKKERLCFKLDKP
                     DWNI"
     gene            3239..3493
                     /locus_tag="PLE23_08045"
     CDS             3239..3493
                     /locus_tag="PLE23_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83250.1"
                     /translation="MKFKSLVIAGIALLSLSVTACKSKPKLLTPEEVTKMVDEKVAKE
                     KEVLGPELDKLCEAQTPQYVKEAVDKLVADAAAAQATPAQ"
     gene            3523..4002
                     /locus_tag="PLE23_08050"
     CDS             3523..4002
                     /locus_tag="PLE23_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83251.1"
                     /translation="MKKYGIIGLFALAVAGFFTLTSADEPMSEEQKQQFDQIDQMVKE
                     QTDAFKAQKDTECRENAMNLAKPKADSIIAATAAAAAKAAAKTPGKVSKPAAKKPAAK
                     PAAPKKETVGQGKPKLGGQSSSTTVGQGKPKLGGEQPAAKGAEGSTIGQGKPKLGGK"
     gene            complement(4526..5038)
                     /locus_tag="PLE23_08055"
     CDS             complement(4526..5038)
                     /locus_tag="PLE23_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83252.1"
                     /translation="MKKLQILFLAFILGSVAVTFTSCGSDDPCKDVDCGTNEDCFEGD
                     CICNVGYEQDANGKCTVEQRAKFIGTYTGSETCTTGSDNYTINITTSSSAIDKVIISN
                     VYNQGITLTGTVNKTALTIPVQTITIGALTYTFQGTGTYTAGTPPTILVDYSIQGNPS
                     NACKFNGTKQ"
     gene            5180..6151
                     /locus_tag="PLE23_08060"
     CDS             5180..6151
                     /locus_tag="PLE23_08060"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83253.1"
                     /translation="MMKSIFTTLLLFIFCIALQAQANPVYLQINPTISKAPLAMIKGQ
                     YSAPNGVKYKLKRLQYYISGVTIFHDGNQETKCDQVYLLINPDQTDYYLGDYNINNVE
                     RIVFNIGVDSVPNHGDPTAWPAGHALAPQNPSMHWGWAAGYRFAAIEGNSDSGNGQFA
                     DLFEFHTIGDELLTKVEVNASSEDNAGKKLITLYADYNKLFTNIDLFGGKIQHGNLTP
                     NDILMANFKDVYYTSPSVATKDVNSHLIGVNVVSPASGLHINYTSDIKGMNFRLMNTN
                     GQALVQQNNISASGSISSDIELPAGVYIYNFYTENTSIATGKIIITK"
     gene            6163..>6650
                     /locus_tag="PLE23_08065"
     CDS             6163..>6650
                     /locus_tag="PLE23_08065"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HMX83254.1"
                     /translation="MKSGFYLIFCSLIFVCCSKDEKAGLYDFIQVPFGFDKPKIPADN
                     ELTEDRIKLGKLLFYDKLMSRDTSLSCASCHKPELAFTDGLPKSVGIDGKFVMRNAST
                     LTNVIYNPYLLSEGGVPTLEQQILVPIMEHNEFDTNILILAERLNNRKDIIDLSLKAY
                     GRP"
BASE COUNT         2272 a         1191 c         1275 g         1912 t
ORIGIN      
        1 ggtgacggat attctcataa tctttatatc aaccacattg cagaatttac ttttcgcttc
       61 aattataccc acgacagcaa aatcggccac ctcttaaaaa gtcgtgcaca caaaacatac
      121 atcctctaca atagatttga cagcccaaaa aatggaaatc cgagccgcga aattgattta
      181 ccaaatggcg gtctggccat cttcatcggc aatatcatcc gacaaaccca aaatgccgag
      241 aattcgaatc ttgtaggcta tggtctggaa ggtcttacca atacagctcc gcatgacatt
      301 attatgataa acaataccct tatcaatgaa aaagcaaacg gttcatttgt tcatgtacaa
      361 aatggtacca atctgttgaa aatgtggaat aacatactgg ctggcggagg cagcaccatc
      421 aatcagagtc cgacaattct tgataccatg tccaattatt acactccgaa catatcagat
      481 gccagttttg tagatgaaaa taatcaagac taccatttaa ctacaggttc atgggctgcc
      541 aaccatgggg cagatccggg ctattaccaa gattatcagt tgcttgcatt gtatgaatat
      601 gatgaagttt tagggaaaat agaccggaaa ttcattgata aaatagacat aggtgcattt
      661 gaatcagata tttcgaccac aatacaagaa acgaaacaga gccattccct taacataaaa
      721 ggcaactgca taaccaatac aggaattgca ggattagcaa tgaaaatttc ggcagttaca
      781 gggtcggata tatattccgg gctaataggt gccggagaac aaaaatgttt tgagaatctt
      841 gttcccggaa tctacatcat tacagccaga accaaagacc ggctggtggc ttctagaaaa
      901 tttttaataa actaaaccaa atatggagca gcaatattat catcttgaca gttttgaatt
      961 atttggtaaa aaattttata gaataatcat acttcccgac gaaactgtct tcatcgaatt
     1021 gaacaaaacc gtaaaaggat gtggaagtaa acccgacgga tttttttgga agaaagtaat
     1081 ggaatacggt gccataaaat gggcagtaga agaattggaa aattttgatt ttgatgcaga
     1141 aaaggaagct gctatagttt gtcacgaatc gaaggacacc ttgctgcaac tttgctctaa
     1201 gacatttgaa atgcttgaga atccggcatt attcaaaagc attatatcgg aaatacaagc
     1261 tatttcactc aggtctgatg aagatgatct gcttgatttt caggaataaa gctttgttcg
     1321 tatcgttttt tgtagtaata attggcaagt acgccaccga taatgaagag gatacctacg
     1381 atggtagaaa taccatacat ttcaccaaaa aataaaatac cgaaacccag cgcataaagt
     1441 actccaagat agttcagtat tgacacatct gccaatctct cttcctgcag tgccagagta
     1501 aggcttgtct gaccaagttg ggtgaggata ccgatggcca gaagtatcag ccattggttg
     1561 ccggacggca tttgccattg aaacattgca gctaccagcc cagctacaac accgaataat
     1621 tgaaaatgca atactaccac gattgggtct tctgattcct tcatactgcg tacataattg
     1681 taggcaaatg cagatgcaac cgaagaaata agcccaattg taagcatttg ggtagaaata
     1741 gtaaagtcga atcctttaat aataagaacg ccggcaaatg aaactgcgaa aaaaagccat
     1801 tggaggggtt ttacagattc ttttaccaaa aagatgccaa tgatactcgt aaatacggga
     1861 gaaatatatt gaatagtgac tgccgtacca agaggaagag ctttaatggt gataaaataa
     1921 gtaaacaagg caagcgtacc aaaaaatcct cgcaacaaca gtagtttccg attgtggcct
     1981 aaccaagaaa ccttcttacg tgctagcaaa ccaaaacaaa aaatggctgc cagcagacat
     2041 ctgaagaaaa cgatttccat cggagggata tccgttaaca ttttgatgag cacgttcata
     2101 actgcaaaat agaatgtact tagcagcatt tgtttaacac cgtaggaaag tttcaagcag
     2161 acaaatattg aattaatacg caaaagtatg ttgtatattt gtcattgtat aatgaaatgt
     2221 cctttaacca ttagaattat ttgaaagagg ctacatgcag caaggcacac ctaacattat
     2281 ttttgaagat aaccacatca tcatcgttga taaaccacac ggctggctcg tgcatggcga
     2341 taagacaaat gatcagacga tcggtgattg gctcaaacaa tacatcaaac aaaagtacaa
     2401 caaaccgggc gacgtgtttc taggtacagt acacaggcta gatcggccgg ttggcggctt
     2461 aatggtgttt gcaaggacat caaagagtct tgaacggctc aataaatcat ttgctgataa
     2521 acatgttaag aaatattacc aggcgctcgt tcaaaattgt cctgaaaagt taagtgctta
     2581 ccttgttcat tggttgagga aggaagaagt cagaaactcg gttgagctca ggaatgatgc
     2641 tgcagggcag gagtggaaaa aagcagaaac caactatcag gttcttggta agtataatca
     2701 atatttttta attgcattag aacctattac cggtcgaaag catcagctac gtgctcagat
     2761 ggcatctata ggctcgccca tagtaggtga tctaaagtac ggatcgaaaa tcagatgttc
     2821 ggatggcaac attgccttaa agtgtaccgg tctcgagttt atacatccgg taaagaagga
     2881 acgactatgt ttcaagctgg ataagccgga ttggaatatt tagcatattt aatgtacaat
     2941 attattaata acctaaatga attttaaaaa aaactataac caaataaaca ttactagctt
     3001 ttagggtcgg tttgttttag aatattttag aaagaatagc tatatttttt catttaacat
     3061 cttcaattat ttttctgaat tagtggaata cctgtatagg tgtggagcta gaatcagaag
     3121 tattattgta ttatcattaa caattctttg caggatatta ttttttgttt agaatttctt
     3181 gagtaaattt gcgttttatc tttacattat tattttttaa ttactaaatc tgacaaaaat
     3241 gaaatttaaa tctttagtca ttgcaggtat agcactgttg tcattatcag tgactgcttg
     3301 taagagcaaa ccaaagttgc ttacacctga agaagtaaca aaaatggttg acgaaaaagt
     3361 tgccaaagaa aaggaagttt tgggtccgga gctcgataaa ctttgtgaag ctcaaactcc
     3421 acagtacgtt aaggaagctg ttgataaact tgtagcagat gctgcagcag cacaagccac
     3481 accggcacaa taatttttta acaattttaa aaaagactta aaatgaagaa atacggaatt
     3541 atcggtctct ttgcacttgc agtagctggt ttttttacac tgacatcagc agacgaacca
     3601 atgagtgaag agcaaaaaca acagtttgat caaatcgatc aaatggttaa agaacaaacg
     3661 gatgcattca aggcacaaaa agatacagaa tgcagagaaa atgcaatgaa ccttgctaaa
     3721 cctaaggctg attctatcat tgctgctact gctgctgctg ccgcaaaagc tgctgcaaaa
     3781 acacctggaa aagttagcaa accggctgct aaaaagcctg ctgcaaaacc agctgcacct
     3841 aaaaaagaaa cagtgggaca aggcaaacct aaactcggtg gacagtcaag cagtacaacc
     3901 gtaggtcagg gtaaaccaaa actcggtggt gaacaacctg cagcaaaagg tgctgaaggt
     3961 agtaccatcg gccaaggtaa accaaaattg ggcggtaagt aatttttcgc tttgtaatat
     4021 tacaaaaggc aggttgatta agttcaacct gcttttttta ttaatagtat tcgggtctat
     4081 tttttaggat tcaatttatt gaaatcattt accgaaaata ttattgagcc ttattttacc
     4141 ctgagatttt gggctttcaa tactttacac aaagatggta cgaatgctgt ccagagttaa
     4201 aatagcatta ccatttgtgt ggtaggaagt attattttgc tcaacgctaa tgatcggctt
     4261 gtctgtatgg ttgtacattt tttattaagc atacaaaaga tggcttgatg tttataagct
     4321 aactatgtta atttgtcagg caaaaaaata ccacaatgca gtaaactaaa aatgtcaatc
     4381 agttaaccat cagtattgga ttttctaact acattatatg tataatatcc atggaaagct
     4441 aaataatgag atgggattta gcaaaaaaaa atcgccccca taatggaggc gacctttttt
     4501 ttattataag aaaaatcttt taatattact gcttagtacc gttaaattta caagcattgg
     4561 aaggattacc ctggatagag taatcaacca agattgtagg aggagtacca gctgtataag
     4621 tacctgtacc ttggaaagtg taagttaaag caccaatagt gatagtctgc acaggaatag
     4681 taagtgctgt tttgttcaca gtacctgtca aagtaatacc ttggttgtaa acatttgaaa
     4741 taattacttt atcaattgca cttgaagaag tagtaatgtt gatagtgtag ttatcagaac
     4801 cagtagtgca tgtttcgctt cctgtataag taccgataaa ttttgctctt tgttctactg
     4861 tacattttcc atttgcatct tgttcgtatc cgacattaca aatacaatca ccttcaaagc
     4921 agtcttcgtt agtaccgcaa tcaacatctt tacaaggatc gtcagaacca caagaagtaa
     4981 atgtgacggc tacagaacct agaataaaag caagaaataa aatttgaagt tttttcattt
     5041 ttgataaaat tttgatttta aaaaataata gggcaaaggt aataaattaa atgttgatta
     5101 aatacgtctt agtttgaatt tttattattc tattcgaaaa aaagttgtaa ttttgacggt
     5161 aattcataaa aaagtaacaa tgatgaaatc aatttttaca acacttttat tatttatttt
     5221 ctgcattgct ttacaagcac aagctaatcc ggtatattta cagattaatc cgaccataag
     5281 taaagcacca ttagccatga ttaaagggca gtatagtgcc ccaaatggtg taaaatacaa
     5341 gctgaaacga cttcagtatt atatctcggg cgtcactatt tttcacgacg gcaatcagga
     5401 aacaaaatgt gatcaagttt atctacttat caatcctgac cagactgatt attatcttgg
     5461 tgactacaat attaataatg tagaaagaat tgtgttcaat attggtgtag attctgttcc
     5521 caatcatggt gacccaactg cctggccagc tggtcatgct ttggctcctc aaaatccgtc
     5581 aatgcattgg gggtgggctg ccggttacag atttgctgct atcgaaggaa attctgatag
     5641 cggcaatgga caattcgccg atttgttcga gttccatacc atcggtgacg aattattaac
     5701 caaggtagaa gtaaatgctt caagtgaaga taatgccggt aagaaattga ttacattata
     5761 tgccgattat aataaactct ttactaatat cgacttgttt ggtggtaaaa ttcagcatgg
     5821 caacctgaca cctaatgaca tcctgatggc caacttcaag gatgtatatt atacatcacc
     5881 aagtgtagca actaaagatg ttaacagtca ccttataggt gtaaatgttg tgtcacctgc
     5941 ttcaggtctg catattaatt atacgtctga tataaaaggc atgaatttca gactgatgaa
     6001 tacaaacggt caagctttgg ttcagcaaaa taatatttct gcttcaggtt cgatctcatc
     6061 tgatatagaa ttgccggcag gtgtatatat ctataatttt tatactgaaa atacttcaat
     6121 agctacaggt aaaattataa ttaccaaata atattattag gaatgaagtc cggattttat
     6181 ctgattttct gcagtctaat ttttgtatgc tgctcaaaag acgaaaaagc cggactttat
     6241 gatttcatac aggttccatt cggatttgac aagccaaaaa taccggccga taatgaattg
     6301 acagaagatc ggatcaaact cggcaaactg cttttttacg acaaacttat gtcgcgcgat
     6361 accagcctct cttgtgcgag ttgccataaa ccagagcttg cattcaccga cggactgccc
     6421 aaaagtgttg gtattgacgg gaaatttgta atgcgtaatg catctacact taccaatgta
     6481 atatacaatc catatttgct ctcagaagga ggcgtaccca cactcgaaca acaaatactt
     6541 gtgcccataa tggagcacaa tgagtttgat accaatattc tcatacttgc cgaaagactt
     6601 aataacagaa aggatattat tgatttgtcg ttgaaggctt acggcaggcc
//
LOCUS       DAOQQY010000152         6623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_4901_length_6623_cov_6.544306, whole genome shotgun sequence.
ACCESSION   DAOQQY010000152 DAOQQY010000000
VERSION     DAOQQY010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4901_length_6623_cov_6.544306"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="PLE23_08070"
     CDS             <1..750
                     /locus_tag="PLE23_08070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMX83255.1"
                     /translation="LLSSIFVALTILVFCLVMYTPIKKYIPGYGDYNQYAKVAALQDR
                     IRKLEQEVQAQNEYTNNIKKILIGEYETAKDQDEQSVPKELVVPEPVKRIPEDEKLRK
                     SVGKENNSQSVPSYNFTPFDVPIENNTFISPLKGIVSSEFKSIQKHYGIDVIAPKDSP
                     IKAIKDGHVILADWTLETGFTIGIQHSNNIVTFYKHNSSLLKKIGDTVKQGESIAIIG
                     NTGMLTDGYHLHFELWHNGAPVDPAGLMTFN"
     gene            complement(770..1327)
                     /locus_tag="PLE23_08075"
     CDS             complement(770..1327)
                     /locus_tag="PLE23_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMX83256.1"
                     /translation="MAYIESNMMPLGTKAPPFQLRDTVSDKVLTLDELKGDNATVVMF
                     ICNHCPYVIHVNEQLVALAADYIPRGVGFIAISSNDAEAYPQDGPEKMKETALQLNYP
                     FPYLYDESQEVARLYDAACTPDLYLFDKEMKLVYRGRLDASRPKSELPVTGADLRAAI
                     DAILDGRQVNEVQYPSGGCNIKWKN"
     gene            complement(1332..2066)
                     /locus_tag="PLE23_08080"
     CDS             complement(1332..2066)
                     /locus_tag="PLE23_08080"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMX83257.1"
                     /translation="MPYFWILISLISFKMDTKAILNTEPIAMKDYLMGRFEPSKHPDF
                     VRIPDQYSEKKGAFLRKEVLEAFIRMYEAALKDNITLTIVSATRNFDYQKAIWERKWS
                     ELKYKDKLNKQATTDQTKALTILRYSAMPGASRHHWGTDIDLCNLNSAYFNTKKGKRI
                     YEWLQRYAGQYGFCQTYTSKKNSDRTGYNQEKWHWSYQPTSRVFTAEAAILLQDTDFT
                     GFSGCETAPELQIVKKYILGINKDCF"
     gene            complement(2088..2651)
                     /gene="pth"
                     /locus_tag="PLE23_08085"
     CDS             complement(2088..2651)
                     /gene="pth"
                     /locus_tag="PLE23_08085"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMX83258.1"
                     /translation="MKYLIAGLGNPGREYENTRHNIGFKIIDKLANRFDVKFEDDNLA
                     WVTEFKWKGRIFVLVKPTTYMNLSGKAVRYWLQKKSIPTENLLVVLDDLAIDFGKLRL
                     RAKGSDGGHNGLRSIDQLLGGNHYARLRCGIGNNFYKSQQVDYVLGNWNREEEDALPE
                     FIDRAAECVLSFGTIGLNNTMNTFNTK"
     gene            2944..3966
                     /locus_tag="PLE23_08090"
     CDS             2944..3966
                     /locus_tag="PLE23_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HMX83259.1"
                     /translation="MKLYPKDVGSFVRKLTLCKLFNVLLIWSSYVLTKWLRRPVQWGL
                     PMTVSIEPTTACNLRCPECPSGLRSFTRKTGNLKKDLFSKVIDEMGDKLMYLIFYFQG
                     EPYINPAFLEMVAYARRRNIYTITSTNGHFLDDENARATVESGLDRLIISVDGTTQEV
                     YEAYRREGRLEKVLIGAKNVVKWKKHLQSPTPHIIFQFLVVKPNEHQIPDIYKLANEI
                     GVDEVKLKTAQVYDYVNGNELIPDQQKYSRYLKQADGTYRLRHSLANHCWKLWHACVI
                     TWDGYVLPCCFDKDAAHRLGNLHTHHFKHIWHGQPYQNFRKAILKGRSQIDICANCSE
                     GCKVWA"
     gene            4279..5304
                     /locus_tag="PLE23_08095"
     CDS             4279..5304
                     /locus_tag="PLE23_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83260.1"
                     /translation="MQAQKVIIKGRLDFGSSKSFEKVVSLFQHRLENFYKNDTLIKPE
                     EMFDPESHAFIIPRLVTQAFPKTWNNTISAFEYIAQFAVTGNIHAFMSENGNIIRKNY
                     IEPLNDKHAVRAYQNGIKLVDDPAQQGQALQYLDEAITAYDKHAMAYEKRGYVKYLLK
                     QLDGALIDFNKSIGVDDQNPEPYVGKATILGLQEKRQEAISTLDEALKLCFPLQPIYW
                     KIRRIKGHLHLQEGQFQEAAQDFKFFLNKKFDKDNPNYAWQRVIMWDYANALIQLHEL
                     EEAHKWLNLIEETPCTGKKISYKAFYKVRSQLRQNLGLKNWTTDIEKLNELEEGSKQT
                     QDNLILS"
     gene            5370..5735
                     /locus_tag="PLE23_08100"
     CDS             5370..5735
                     /locus_tag="PLE23_08100"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HMX83261.1"
                     /translation="MKDNPISEPKFVQNAEFDERIEALRNQIDTIDHEILALIAERMK
                     VSLKIGHEKSVYNITVFQKERWAQMYDDRLKILTRSGLSVEFSENFLKILHTESIRQQ
                     VEVIKKIQAKANEDGHPTL"
     gene            5740..>6623
                     /locus_tag="PLE23_08105"
     CDS             5740..>6623
                     /locus_tag="PLE23_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83262.1"
                     /translation="MKKYLLLSLILLSFIFAQNIKAQNYIQLKNAPEQLKKDFQNGKE
                     YFDKKDYDKSVKLFSKILIKEPRFIDARIFLAASFDRQKKYDNAIAEYKNVLKTDTLY
                     EPEVLLAIGRLLKTKEQYKDAAYYYARFIALTPATDNKRQMAADEAGVCRFLANAMAD
                     KKDIHLYNLGPTINTSAHEYLPCLSVDGKTLIFTRVVNNNEDFYQSIMNDDGSWSSAE
                     PIHELNTPMNEAGQTISADGRTLVFVACDYPDSYGSCDLYISYLKNGVWTPAKNMGNI
                     VNSTSWDSQPSLSADGKV"
BASE COUNT         2078 a         1324 c         1340 g         1881 t
ORIGIN      
        1 ctgctaagca gcatttttgt tgctcttacc atacttgtat tttgtctggt gatgtacacg
       61 cctatcaaaa agtatattcc cggatatggc gactataatc agtatgccaa agtagctgcc
      121 ctacaagacc gcatccgaaa actcgagcag gaagtacagg cacaaaatga atacaccaac
      181 aatataaaaa aaatactcat cggcgagtac gaaactgcca aagaccagga tgaacaaagc
      241 gtgccaaaag aactcgtagt acccgaaccg gtaaaaagaa taccggaaga tgaaaaactc
      301 agaaaaagtg taggtaaaga aaataattcg caaagtgttc cgtcctataa tttcacgcca
      361 tttgatgtgc cgattgagaa taataccttc atttcaccac tgaagggaat tgtaagctca
      421 gagttcaaat ctatacaaaa acactacggc attgatgtaa tcgcacccaa ggattcaccc
      481 atcaaagcca tcaaagatgg tcatgtaata ctggccgatt ggacactcga aacgggtttt
      541 accataggca tacagcacag taataatata gtcacatttt ataaacataa ctcaagtctg
      601 ctaaaaaaga ttggagatac agtaaagcaa ggcgaatcca tagctatcat cggcaatacc
      661 ggtatgctga ctgacggata tcaccttcat tttgagttat ggcacaacgg tgctcctgtt
      721 gacccggctg gccttatgac gtttaactaa gatttggagt gggcaaagct taatttttcc
      781 actttatatt gcacccaccg ctggggtact gtacttcatt gacttgtcgg ccatctagta
      841 tagcatcaat agcagcacgc agatcggcgc ctgttaccgg tagctcagac tttggtctgg
      901 aagcatccag acgcccccga tataccagtt tcatttcctt gtcaaacagg taaagatcgg
      961 gcgtacatgc tgcatcatag agacgtgcta cttcctggct ttcgtcataa agatatggga
     1021 acggatagtt gagttgaaga gctgtttctt tcattttttc tggaccatct tgtgggtatg
     1081 cctcggcatc attagagctg atggcaatga atccaacacc tcgtggtata taatcagcgg
     1141 ccagtgctac caactgttcg ttgacgtgta ttacgtatgg gcaatggttg cagataaaca
     1201 ttacgacagt agcattgtct cctttgagtt catctagggt taacacctta tcactaacag
     1261 tatcccgaag ttggaaggga ggagcctttg tgcccaaggg catcatattt gattctatat
     1321 aagccataag tttaaaaaca gtctttatta attcctaaaa tatatttctt tacaatttgg
     1381 agttcgggag cagtttcaca accggagaaa ccagtgaaat cagtgtcttg aagcaatatt
     1441 gctgcttcgg cagtaaatac gcgtgatgtc ggctggtacg accaatgcca tttttcctga
     1501 ttgtaaccgg tgcgatcgct gttttttttc gaagtatatg tctgacaaaa accatattgt
     1561 cctgcatatc gctgaagcca ctcgtagatt ctttttcctt ttttggtgtt aaaataagca
     1621 gaattgaggt tgcaaagatc gatgtctgta ccccaatgat gccttgatgc acccggcatg
     1681 gcagaatatc tgagtattgt aagggccttt gtctggtcag tggttgcttg tttgttcagt
     1741 ttgtctttgt acttgagttc cgaccatttt ctttcccaga ttgctttctg atagtcaaaa
     1801 tttctggtgg ccgacacaat ggtaagtgtt atattatcct ttaaagcagc ttcgtacatc
     1861 cgaataaatg cttccagtac ttcttttcgc agaaatgcac cttttttctc ggaatattga
     1921 tcaggaattc ttacaaaatc tggatgtttt gaaggttcga atctgcccat caggtaatct
     1981 ttcattgcta tgggttcggt atttaagatg gcttttgtat ccattttaaa agatatgagg
     2041 cttatcaata tccaaaagta cggcatttgg gacagtattt ggtgatttta tttcgtatta
     2101 aaagtattca tggtgttgtt caggccgatt gtaccaaaac tcagcacgca ttctgcagcg
     2161 cggtcaatga attcgggcaa tgcatcttct tcttcacggt tccaattgcc cagcacataa
     2221 tcgacctgct ggcttttata aaaattgttg ccaatgccgc acctaagcct ggcataatgg
     2281 ttgccaccca agagctgatc gatacttcgt aggccgttgt gacctccatc cgaaccctta
     2341 gcgcgcagac gaagtttgcc gaaatcaata gcgagatcgt cgagtacgac aagcaggttt
     2401 tcagtaggta tggatttttt ctgcagccag taacgtactg cttttccgct taaattcata
     2461 tacgtagtag gttttaccag aacaaaaatt ctgcctttcc atttgaattc agttacccag
     2521 gcaaggttgt catcttcaaa ttttacgtca aaacgattgg cgagtttgtc tattatttta
     2581 aacccgatat tgtgacgtgt attttcgtat tctctgcccg gattgcccaa acctgctatc
     2641 aggtatttca tattacaata tatttacaaa gataatatgc taatgtttag ttcagtatca
     2701 gtagtgtgta tgattgtgaa ataattattt tacaaacttg tttaaaacaa aaagagcctt
     2761 tcgtgtgaaa agctccttac attttacctt atcctgttgc ttgtggcaac aagaataacc
     2821 tacctagcga tgcaaagata cggtcttttc aataaaaatg aaactatttt attttttttt
     2881 atcagggcta aaatgctgcc cggtttactt actaatttct ttgtattaac tttgacaccg
     2941 aatatgaagt tatacccaaa agatgttggt tcctttgtca ggaagcttac tttgtgcaag
     3001 ttgttcaatg ttttgctcat ctggagctcg tatgtactta ccaaatggct cagaaggccg
     3061 gtacagtggg ggctgccgat gacggtaagt atagagccta ctactgcatg caatctgcgt
     3121 tgtcccgaat gcccaagtgg gttgcggtct tttacccgga aaacaggaaa tctcaagaag
     3181 gatttattca gtaaagtaat agatgaaatg ggcgacaagc tcatgtacct gatcttttat
     3241 ttccaaggcg aaccatacat caatccggca tttctggaga tggtagctta tgcccgcagg
     3301 cggaatattt ataccattac atccaccaac ggtcattttt tagatgacga aaatgcaagg
     3361 gcaactgttg aatcaggtct tgaccgtctc atcatctcag tagatggcac cactcaggaa
     3421 gtctatgaag cgtacagacg ggaaggcagg ctcgaaaaag tactaatagg agcgaagaac
     3481 gtggtaaaat ggaaaaaaca tctgcaatcg ccaacaccgc atatcatatt ccagtttttg
     3541 gtagtaaaac ccaatgaaca ccagataccc gatatttaca agctggctaa cgaaataggt
     3601 gtagatgaag taaagctcaa gactgcacaa gtatatgatt atgtaaatgg gaatgaactg
     3661 atacccgacc aacagaagta ttctcgttat ctgaagcaag ctgatggtac atatcgcctg
     3721 aggcattctt tagccaatca ttgctggaag ctctggcatg catgtgtgat tacctgggac
     3781 ggatatgttt tgccttgctg ttttgacaaa gatgctgcac atagacttgg caacctgcat
     3841 acccatcatt tcaaacatat atggcatggc caaccatacc aaaacttcag gaaagccata
     3901 ctgaaaggtc gttctcaaat agatatttgt gccaactgct ctgaaggttg caaggtgtgg
     3961 gcttgagtta ttacaagtgt tcttccactt atgtctattt ctcgtaattt tttatacaac
     4021 taatggagaa cgacttacgt attgtactga ttattaatac taataacaga aaaattttta
     4081 tgaaaaatta aatgaacata gtattgatta aataaaaaaa taatttaact ttgtagttaa
     4141 tcaaaaatca ggtctattta tctccctgat gatggtctga caacaaattc ctgaattcca
     4201 ttttgaaacg tatttattta gtattaatta atatttaacc ccgaatatca ttttaaatac
     4261 caaaaaccaa ccggaatcat gcaggcacaa aaagttatca tcaaagggag actggatttt
     4321 ggaagctcaa agagtttcga aaaggtagta agtttgtttc agcacaggct cgagaatttt
     4381 tataaaaacg atacgctaat caagccggag gaaatgtttg atccggagtc gcatgcgttt
     4441 attataccaa ggttggtgac acaggcattt ccgaaaacct ggaacaacac cattagtgca
     4501 tttgaataca tcgctcagtt tgctgttacg ggcaatatcc atgcatttat gtcagaaaac
     4561 ggcaacataa tacgcaaaaa ctatatcgag cctctcaatg ataagcatgc agtaagagca
     4621 taccaaaatg ggataaaact cgtcgatgac cctgcccaac aaggtcaggc actacaatat
     4681 cttgatgaag ccattactgc ttacgacaaa catgccatgg catacgaaaa aagaggatat
     4741 gtcaaatacc tcttaaaaca attggacggc gcactaatcg attttaacaa aagcataggt
     4801 gttgacgatc agaatccgga gccttatgta ggaaaagcta ccattcttgg cttgcaggaa
     4861 aaaaggcagg aagcaattag tacgcttgat gaagcactta aactctgttt cccattacag
     4921 cctatttact ggaaaattag acggataaaa ggacacttgc acctacagga aggtcagttt
     4981 caggaagcgg cacaagattt taagtttttc ctcaacaaaa aatttgacaa agataaccct
     5041 aactacgcat ggcaacgtgt cattatgtgg gactatgcca atgccctgat acagttgcat
     5101 gaactggaag aggcacacaa atggcttaac ctcatcgaag aaacaccatg taccggtaaa
     5161 aagatttcgt acaaggcgtt ctacaaggta cgttcgcaac tcaggcagaa cctcggacta
     5221 aaaaactgga ctacggacat cgaaaaactc aatgaactgg aagagggatc aaagcagact
     5281 caagataatc tgatactctc ctgaatataa agaataattt tcgctcttat ggtatttcgt
     5341 tttttatcat aaatagcatt tagcgtgaca tgaaagacaa tcccatatct gaaccaaagt
     5401 tcgtacaaaa tgcggaattt gatgaaagga tagaagccct gcgaaaccag attgatacaa
     5461 ttgaccatga aattttggca ctcatagccg aacgaatgaa agtgtcacta aaaatcggac
     5521 atgaaaaatc agtttacaat ataacggttt ttcaaaaaga aaggtgggca cagatgtacg
     5581 atgaccggtt aaaaatattg acaagatctg gcctgtcggt agaattttct gaaaattttt
     5641 tgaagatact acatactgag tctatccgac agcaagtgga agtaataaaa aagatacagg
     5701 caaaggccaa tgaagatggc cacccgacgt tgtagtatta tgaaaaaata tctgttactc
     5761 agtctgattt tgctttcctt catatttgca caaaacatta aggcacaaaa ctacattcag
     5821 ctgaaaaatg cacctgaaca gctaaagaag gattttcaaa atggcaagga gtactttgac
     5881 aaaaaagatt acgataagtc ggtaaagctg ttttcaaaga ttctgattaa agaaccgcgt
     5941 tttattgatg cacgcatatt tctcgcagca tcatttgatc gtcaaaaaaa atatgacaat
     6001 gccatcgcag aatacaaaaa tgtacttaaa accgatacct tgtacgaacc agaggtactc
     6061 ttggccattg gccggcttct gaaaaccaaa gagcaatata aggatgccgc ctattattat
     6121 gcacgattta tagcgctgac tcctgctaca gacaataaaa ggcaaatggc agcagatgaa
     6181 gccggtgttt gtcggtttct tgcgaatgct atggccgaca agaaagatat tcatctttac
     6241 aatcttggac caacgatcaa tacttctgcc catgagtatc ttccttgtct atcagttgac
     6301 ggtaagaccc tgatatttac aagggttgtc aacaacaatg aagattttta tcaaagtatc
     6361 atgaatgatg acggaagctg gagtagtgca gaacccatcc atgagctcaa cacacctatg
     6421 aatgaagccg gtcaaaccat ctctgccgat gggcggacac ttgtttttgt ggcttgtgat
     6481 tatccggatt cgtacggtag ctgtgaccta tacatcagct atctgaaaaa cggagtgtgg
     6541 acaccggcaa agaatatggg caatatagtc aattccacct cctgggattc acaaccttca
     6601 ttatctgcag atggtaaagt att
//
LOCUS       DAOQQY010000153         6513 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5026_length_6513_cov_5.493651, whole genome shotgun sequence.
ACCESSION   DAOQQY010000153 DAOQQY010000000
VERSION     DAOQQY010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6513
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5026_length_6513_cov_5.493651"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLE23_08110"
     CDS             complement(<1..532)
                     /locus_tag="PLE23_08110"
                     /inference="COORDINATES: protein motif:HMM:NF027499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4874 domain-containing protein"
                     /protein_id="HMX83263.1"
                     /translation="MNGAINFYSKWIVLVVILLVSDLSGQTVLYKPSTEVIANPGRGL
                     YKYSITDEEYSSVVGYNNLAPAFLTEWKNSPDKITVVYRTFLLSSFIDKDINPVYLDN
                     IRTDFDHVRQAGMKIIVRFSYSDTQDNAPQQPKKYRILSHISQLSSVLNQNKDVIFSV
                     QAGFIGTWGEWYYTNSD"
     gene            complement(587..1282)
                     /locus_tag="PLE23_08115"
     CDS             complement(587..1282)
                     /locus_tag="PLE23_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83264.1"
                     /translation="MKPALIFKTIILCLLASLFLSSCSPKTQFKGFKDGPYSLKLSPS
                     KNKDSKNISVGYSLTTQEGKMISGKIDDGNKVFIVKNHLDDVRLDYIECDSFTVNAIP
                     NNLWLSCVEVVRKCNTSNSCGTGKLNVRGTVSNRSNPSSTISIFDGNVYGPNGSVQNQ
                     NNSYNACFAHVGGTVRIGFAISSSETVVVVLNNVAPDKDVEVNVVLTEAQPMIGGNFD
                     CSTTGPRGPSGGQ"
     gene            complement(1320..3536)
                     /locus_tag="PLE23_08120"
     CDS             complement(1320..3536)
                     /locus_tag="PLE23_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMX83265.1"
                     /translation="MVIGLVFLCQLQYAQNRGLTPELSKNQEVRQGKTYGIVIAISDY
                     SRYRKLDFAHNDGSAFAGWLESKSGEIRDTNNLRVLLDKDATFSSVVNAFDWLNSKCR
                     DGDKAYIYFAGHGDVECKSASEPGYLLCYDSPERLFASGGAMPLNYLQEMVTTLSMKN
                     TKVVVVLDACHAGKLAGDVVNGRQRAAENMIFYVPDPNVVKILSCKANQNSREDPTLG
                     GGRGVFSYFLTNGLMGMADANRNDKVNIAELERYLEDHIMTTTAPIMQEPIIKHSGSK
                     MDILVKVNPFVRDSLLNEQQIQSFASIRARSIYEHSAIGADTIALPDLQHFQTCLKEQ
                     RFFEPAQDCAEYYYAKLLALAVSDDFKNEVTRNYAVALQEKAQRIINNFIEVKVAELS
                     KSYKSGYEDYKVLPKFLDRATALLGESHYFYKNLKSNQYLFEAFAMQYEGFGIFDSVR
                     IRKNLNLYKKSLEYNDKNPLTLYFISKYYAGKLHDRDSCDIYFKKLTEVATTWVMAYA
                     QHADNLVRYFKDFEQAKSTVLEGWAIDSTNTYLIKALANIYFYQKQYAKAIQKYRDAE
                     RLDSSDVLTYVNLAACYTMTGEDSLAIVNYEKSIKMDSTLMVVYMNYGWLYFKHKDYD
                     NAERIFLKGVNLRNEYVPVKGRLCQVYLAQNKLGKVKEQLKLIKAIYPDDWRYYLYSA
                     CIEAKKGQDNKALELLRKAMKSDGFDDMRTVEDSGYFEKLKKDGRLAKLIEEVFEK"
     gene            complement(3655..4374)
                     /locus_tag="PLE23_08125"
     CDS             complement(3655..4374)
                     /locus_tag="PLE23_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83266.1"
                     /translation="MAQNKPAKPLTQKINGENISELTYRAPSKERLNELFYNLPEVPN
                     YDIQNAFDNLSNGNRAAAKDVFTRFATTDWQAAYGLAIVFSLDNQLEQAIEVLNVALL
                     NNPDNRSLLFKLGTYYNKQQNYQKAEEQFIKMVNLDVDDRDAWYELGMLYLCIPSLRN
                     FAEFSMTKVLNLYPSDMNAPFELARLYSREGDTNRALILLDKAVENGFKNRKLMAEDK
                     DLANLRDMKEYEQLMKMRFGS"
     gene            4693..5289
                     /locus_tag="PLE23_08130"
     CDS             4693..5289
                     /locus_tag="PLE23_08130"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HMX83267.1"
                     /translation="MEIKNYKIEEKLISATMANIYGLLMMIPVCIVFVTPFYLIWPDR
                     LRQANFEELFARTFGQPTGVTSFFVIILIFLVSVVLHELIHGITWSLFTKNGFSSIRF
                     GINWKMLMPYCHCKEPLLLRHYITGAIMPFILLGLVPTICAIFTGSFLLLVTGLIMTI
                     SAIGDFMIIALIRKYPGDTLVLDHPNEAGCYIYLRENG"
     gene            complement(5319..5960)
                     /locus_tag="PLE23_08135"
     CDS             complement(5319..5960)
                     /locus_tag="PLE23_08135"
                     /inference="COORDINATES: protein motif:HMM:NF008102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMX83268.1"
                     /translation="MIDLIKQFIDFVLHIDKHLIELASQYHQWTYLIIFLIVFCETGL
                     VIMPFLPGDSLLFAAGALCATGALNIWVLIIGVFSAAVLGDNINYVVGKFIGRKVSEG
                     NGRFIKKDYITRTEKFYEKHGGKALIMARFAPILRTFAPFVAGAGRMEYPRFLTYCIV
                     GNLLWVNIFCWVGYLFANNEFVKNNFSVVVLGIIFVSLIPVVLAWWKSRKTAN"
     gene            complement(5957..>6513)
                     /locus_tag="PLE23_08140"
     CDS             complement(5957..>6513)
                     /locus_tag="PLE23_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HMX83269.1"
                     /translation="VALIVFMLVVLLPVALMIKIDIKRFLQLITEPVTLAFATTSSEA
                     ALPKAMAAMEKFGVPRKIVSFVMPMGYSFNLDGTTLYLSLATIFVAQAAKVELSIAHQ
                     FAIVLTLMLTSKGVAGVPRASLVILLGTATSFGLPVEPIFIILGIDELMDMARTSVNV
                     IGNCLATAVVAKWEGELETPNPEA"
BASE COUNT         1918 a         1354 c         1201 g         2040 t
ORIGIN      
        1 catcggaatt ggtatagtac cattcacccc aagtgccaat aaagccggct tgaacagaga
       61 aaataacatc cttgttctgg tttaacacag acgaaagctg agaaatatgc gacagaatcc
      121 tatatttttt gggttgttgt ggtgcattgt cctgtgtgtc agaatatgaa aatctgacaa
      181 ttattttcat acctgcctgc ctaacatgat caaaatctgt gcggatgtta tccagatata
      241 ctggattgat atctttgtcg ataaaagatg atagtagaaa agttctatac accacagtaa
      301 ttttatccgg actgtttttc cattctgtca agaatgccgg ggcaagatta ttgtagccaa
      361 ctaccgatga atattcttca tccgttattg aatatttata caacccccgt ccagggttgg
      421 caattacttc agtagatggt ttgtacagaa ctgtttgccc cgacaagtca gatactaaca
      481 atatgactac taaaacaatc cactttgaat aaaaattgat agccccattc attttaagca
      541 tcagacaaaa cacccgaaat taatttcaaa tcatgctcga tttcaattac tgcccacctg
      601 atgggccacg aggaccggta gtactgcagt caaaattacc accaatcatc ggttgggctt
      661 cggttaatac tacatttact tctacatcct tatccggtgc cacattatta agtacaacca
      721 ctactgtttc ggaacttgaa atggcaaaac ctattctgac tgttcctcct acatgtgcaa
      781 aacaagcgtt ataactattg ttttgatttt gtacagaacc atttggtcca taaacattgc
      841 cgtcaaatat gctgatggta ctggaaggat tgcttcggtt agaaacagtg ccacggacat
      901 ttaacttgcc cgtaccacag gaattactgg tattacattt tcgaactact tcaacacaac
      961 tcaaccatag gttatttggt attgcgttga cggtaaatga gtcacattct atatagtcca
     1021 gtcgcacatc atcaagatgg ttttttacaa tgaagacttt atttccgtcg tctattttcc
     1081 cacttatcat cttaccttct tgcgtcgtta aggaatatcc aaccgatata ttctttgaat
     1141 ctttattttt tgaaggtgaa agctttaggc tataagggcc atctttaaaa cccttaaact
     1201 gtgtcttggg gctgcaagag gataaaaata aagatgcgag taaacacaaa atgattgttt
     1261 tgaaaataag tgctggtttc atgattggtt atgtttatat tatattaaaa aatgtgtgtt
     1321 tatttctcaa aaacttcttc gatgagtttt gcgaggcggc catctttttt aagcttctcg
     1381 aagtatccgc tgtcttccac tgttctcata tcatcaaatc catcactctt cattgccttt
     1441 ctcagcagtt cgagtgcctt gttgtcctgt ccttttttag cttcgataca tgcactgtac
     1501 agatagtatc tccagtcgtc cggataaatg gcttttatca gtttgagctg ctccttcacc
     1561 tttccgagtt tgttttgagc caagtacacc tggcataacc gacctttcac cggtacgtat
     1621 tcattgcgta ggtttacacc tttgaggaat attcgttctg cattatcata gtctttatgc
     1681 ttgaaatata accagccgta gttcatgtac acgaccataa gagtagagtc cattttgatt
     1741 gacttttcgt aattgacaat agccagtgag tcctctcctg tcatggtata gcatgctgca
     1801 agatttacat aagtaagtac atctgaagaa tcgaggcgtt ctgcatcacg gtatttttga
     1861 atggcctttg catactgttt ttggtaaaaa taaatgttgg caagtgcctt tatcaggtaa
     1921 gtattagtag aatcaatggc ccatccttct aatacagtac tcttggcttg ttcaaaatcc
     1981 ttaaagtacc gaacgaggtt atctgcatgc tgggcataag ccattaccca agtagtagct
     2041 acttctgtga gttttttaaa ataaatatca caagagtcac gatcgtgaag tttaccggcg
     2101 taatacttgc ttatgaagta taatgtaagt gggtttttgt cattgtattc cagtgatttt
     2161 ttgtacaagt ttagattttt acgaatacgt acagaatcaa atatgccaaa gccttcatat
     2221 tgcatagcaa atgcttcgaa aagatactga tttgatttca ggttcttata gaaataatgg
     2281 ctttcgccaa gaagggcagt cgctctgtca aggaattttg gcaaaacctt gtaatcttca
     2341 tagccggatt tgtaggattt tgacaactca gccaccttca cctcgataaa attgttgatg
     2401 atacgctgtg ccttctcctg aagtgctaca gcataattac gagttacttc gtttttgaaa
     2461 tcatcactta cagccaatgc aagcagcttg gcataataat attcggcaca gtcttgagcc
     2521 ggttcgaaaa accgttgttc tttcaagcaa gtctgaaaat gctgcaaatc gggtaatgca
     2581 atagtatctg cacctatggc tgaatgttca tagatagacc ttgccctaat actcgcaaag
     2641 gattgtatct gttgctcatt aagcagagaa tctcttacaa atggattaac ttttaccaat
     2701 atatccattt tgctgccact atgcttgatg atgggctctt gcataatggg ggctgttgtg
     2761 gtcatgatgt gatcttcaag gtatcgttcg agttctgcga tgtttacttt atcatttctg
     2821 ttggcatctg ccatccccat caaaccattc gtaagaaaat aactgaatac acctctgcca
     2881 ccgccgagtg ttgggtcttc tcttgaattt tggtttgcct tgcaggaaag tatttttaca
     2941 acattagggt ccggcacata aaaaatcatg ttttcggctg ctctctgacg gccgttgacc
     3001 acatcgcctg caagtttgcc tgcatgacaa gcatcgagta ctactaccac ctttgtgttt
     3061 ttcatagata gtgtagtcac catttcctga aggtagttaa gaggcatagc gccaccacta
     3121 gcaaacagtc gttcgggcga atcgtaacaa agcaggtatc ctggttcact ggcactttta
     3181 cattcgacat ctccatggcc tgcgaaatag atatacgcct tatcaccatc acggcatttc
     3241 gaattcagcc agtcaaaagc atttactaca gatgaaaatg ttgcgtcttt gtcaagcagt
     3301 actctaagat tattagtatc cctgatttca ccactcttag attctaacca accggcaaaa
     3361 gcacttccgt cattgtgggc aaagtcaagc tttcgatagc gtgaatagtc tgagatagca
     3421 attactatcc cataagtctt tccttgcctt acctcttggt tctttgaaag ctccggtgta
     3481 agacctctat tctgggcata ctgcaattgg catagaaata ccagcccgat caccatcaat
     3541 atgtgtcgta gattcttcag tttcgtaaaa ttttattttc taaaaaaaaa ctaaaagtat
     3601 ttcttgttga cgtatcttct atatctctta aaacgttcca acgtcgaaat ttaatcagga
     3661 tccaaagcgc attttcatca attgttcgta ttccttcata tcacgcagat tggccaggtc
     3721 tttgtcttct gccatcagtt tccggttttt gaatccgttt tctactgctt tatccaataa
     3781 tatcaatgca cggtttgtgt caccttcacg cgaatacagc cgggcaagct caaatggtgc
     3841 attcatgtct gacggataaa ggtttaatac cttggtcatt gaaaactctg caaaattccg
     3901 caaagacgga atacacaggt atagcatacc aagttcgtac catgcatcac ggtcatccac
     3961 atccagatta accattttta tgaattgctc ttctgccttt tggtagttct gttgcttgtt
     4021 gtaataagtc ccgagtttaa acaaaagaga acggttgtca ggattgttga gcaaggctac
     4081 atttaatact tctatagcct gttctagctg attatcaagt gaaaatacaa tggccaaacc
     4141 ataagcagcc tgccaatcgg ttgttgcaaa tctagtaaat acatcctttg ccgcagcacg
     4201 gttgccattg ctcaggttat caaaggcatt ttgtatatca tagtttggca cctcaggtaa
     4261 gttgtaaaac aattcattca atctttcctt agatggtgcc ctataggtaa gctcagaaat
     4321 attttctcca ttgatttttt gcgtcaatgg ttttgcaggt ttgttctgag ccattatgca
     4381 aaaaggaatg gtcaatatta taaacaaaaa aagaaacttc aacttcataa ataatctcct
     4441 gactaagtta cttcaaattg attctacaca ttaatacaaa tataaactac tatcttaaca
     4501 aatttccaaa aattttgcaa ataattttta cagatgctta ttttactatg atcgtaaata
     4561 agcctgtaat attaaattgg tatatctgcc tagtcattac atttaataaa acgctaattt
     4621 attaagcata taaagctatt tttgaccatt aaataactca tctggacatt accagtaaca
     4681 caaataaata ttatggaaat taaaaattac aaaattgaag aaaagttaat aagcgctacc
     4741 atggccaata tttacggact tttgatgatg ataccggtct gcatagtatt tgtcactccc
     4801 ttctacctaa tatggcctga caggttaaga caagcaaatt tcgaagaatt gtttgcccgt
     4861 acttttggtc agcctacagg agtaacttcc ttttttgtca tcatattgat atttttagtt
     4921 agcgttgtat tacacgaact aattcatggt atcacctggt ctttatttac aaaaaacgga
     4981 tttagctcaa tcagatttgg aatcaattgg aaaatgctga tgccttattg ccactgtaaa
     5041 gaaccattat tgctcagaca ttatatcacc ggcgcaatca tgccatttat tttgcttggg
     5101 ctagtaccaa caatatgtgc aatttttaca gggagttttt tattacttgt aaccggattg
     5161 attatgacca tctcagctat cggagatttc atgattattg cattgatacg gaagtatccg
     5221 ggcgatacac ttgtacttga ccatccaaat gaggccggtt gctatattta tctgagagaa
     5281 aacggataaa actgaacact tccgttgttg ggttttactt agttagcagt ttttctactc
     5341 ttccaccatg ccagtactac aggaatgagg gaaacaaaaa tgatacccaa gacgacaacc
     5401 gaaaagttat tctttacgaa ttcattatta gcaaaaaggt aaccaaccca acagaaaata
     5461 ttgacccaca gaagattgcc cacaatgcaa taagtaagga agcgaggata ctccatcctg
     5521 ccagcccctg ctacaaatgg tgcaaatgtc cgtagaatgg gtgcaaacct ggccattatg
     5581 agtgccttgc caccatgctt ttcataaaat ttttcggtgc gcgtgatgta atccttcttg
     5641 atgaatctac cgttgccttc tgatacttta cgaccgatga atttacctac tacgtaattg
     5701 atattatcgc ctaatacagc agcagaaaaa actccgatga tcagtaccca aatattgagt
     5761 gctccggtcg cacaaagcgc acctgcggcg aacagcagcg aatcgccggg gagaaacggc
     5821 atgataacaa gacctgtttc gcaaaacaca atcagaaaga taatcagata agtccattga
     5881 tgatattgac ttgccaattc aatcaagtgt ttgtcaatgt gtaatacgaa atcaatgaat
     5941 tgtttgataa ggtctatcat gcttctggat ttggagtttc taattcgcct tcccacttag
     6001 ctactacagc tgtggcaagg cagttgccta ttacattaac ggaagtacgt gccatgtcca
     6061 tcagctcatc gatacccaat atgataaata taggctctac gggcaggccg aaagaagtgg
     6121 cagtaccaag caagataaca aggcttgctc taggcacacc ggctacacct ttgcttgtaa
     6181 gcatgagtgt caacacgatg gcaaactgat gagcaatcga caattctacc ttggctgcct
     6241 gtgcaacaaa gatagtagcc aagctgagat aaagtgtcgt gccgtcgaga ttaaagctat
     6301 aacccatcgg cattacaaat gatacaatct tacgcggcac gccaaatttt tccattgccg
     6361 ccatagcctt gggtagtgca gcctccgagc tggtagtagc aaatgccagc gttacaggct
     6421 cagtgatgag ctggagaaat ctttttatat ctatctttat catcaatgcc accggtaata
     6481 gtactacgag catgaaaaca attaatgcca cat
//
LOCUS       DAOQQY010000154         6507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5030_length_6507_cov_5.440484, whole genome shotgun sequence.
ACCESSION   DAOQQY010000154 DAOQQY010000000
VERSION     DAOQQY010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5030_length_6507_cov_5.440484"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /locus_tag="PLE23_08145"
     CDS             complement(<1..1233)
                     /locus_tag="PLE23_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HMX83270.1"
                     /translation="MSNLLFAQQQDTISGKFIIVEKAETFYYKKINGLEYQWMVGKVR
                     LRQGNTYMSCDSALLLNNQLKAYGRVLIQQTDTIHVFSDSLWYNGDTKDADLFGDVVL
                     VNGTQQLFTSRLNYNLDTKIAKYDNKAILNDGKTKLSSRTGMYSVNEKKAFFQKDVRI
                     VDSTFTLKADSLEFLTDRRTAVFKGPTLIVQDTAKIYCEAGFYNIPNRKAEFTHNAQY
                     EGKDTKATADTIRYEGYLAEVSLVENARYESSTEKASAQRIVHNEQSKLTSLYGNAKY
                     RKGKQDFAGEEIHYNGNDGSFKTKGRSRVSEPPQIMEADFMDYSKAKGFGTASGNVIW
                     QDTSARLITKCDTTVFDRSNDYLKAFGRRPLLISYDGADSTFLTSDTLKAFRMIRDSV
                     DTTRIFTAYHDVRILRRDL"
     gene            complement(1285..2451)
                     /locus_tag="PLE23_08150"
     CDS             complement(1285..2451)
                     /locus_tag="PLE23_08150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX83271.1"
                     /translation="MQLSYWERKSFMSDFDTVIIGAGIVGISAALSLIEHDPSMKILI
                     IERGFLPSGASTKNAGFACFGSVSELLDDFDKMGEESTLNLVARRFAGLAALRKRTGD
                     DEIFFRPGGNYEVFKTDEEDFFSHCHDNIHRLNRLVKEITGVDDTYSICDKKIQNFGF
                     SDVSHLILNKCEGRLDTGMMMQKLLTLARTAGIVFYFGAEVRQIEDESTKVTLRLQSE
                     YYVEAKQLIVATNGFARQLLPELEVFPARNQVLVTHPYKNMKLDSCFHYDLGYFYWRE
                     IDGRILLGGGRNLFPDTEYTDSLSINPDIQEHLVQMLKTWITPHEFPGIDMQWSGILG
                     IGKVKFPIIKKISENVIVAVRMGGMGVALGTLAGHEAAELMLGISTARTTDVVS"
     gene            2566..3012
                     /locus_tag="PLE23_08155"
     CDS             2566..3012
                     /locus_tag="PLE23_08155"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HMX83272.1"
                     /translation="MIQRIQSVYLVLAVIALAILFFPISFGDLKAGSTIAKTPALSDG
                     TLNLEDNNLSMICTFAGIALFMAAIFLFKNRRLQTRIVALSMLVALIIFILAGWLFMS
                     SSKIAGNDLSPGIGLGSPVIAILFGWLANRSIRKDENLVRSMDRLR"
     gene            2975..4213
                     /locus_tag="PLE23_08160"
     CDS             2975..4213
                     /locus_tag="PLE23_08160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX83273.1"
                     /translation="MKTLFGLWTDFVKKTSATLLMKKTPLFTLLLTIFIDIVGFSIII
                     PILPVYSKEIGASNIMVGIIAGSYSLMNFLFSPVWGNISDRIGRKPVILVSLFITACS
                     YLLFAYSKTISLLLISRIIAGIGSANYGAAQAYISDITKPEDRARAFGYVGAAFGLGF
                     IIGPVIGVFILKMWNVAAIGYVTMALNLANMLMAMAILHETRKEVKSGTPLLENPIIN
                     VRDGLKTPVLGKLMFISLLYITALSMMQITISVFWSEKYGLSKTQISGMFAFMGIMSV
                     LVQGFLVGKLTLKFGEKNMMIIGAVLLAAGLYIIPMAPYKEGFYLVEGFAIILISLAN
                     GCLSPSITSLLSQNAGAEEQGKVLGVGMSVNSLGRVIGPYAGGFLYGMAMFMPYFIGT
                     LIMIGAWNVARRFRTKYAHI"
     gene            complement(4442..4579)
                     /locus_tag="PLE23_08165"
     CDS             complement(4442..4579)
                     /locus_tag="PLE23_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83274.1"
                     /translation="MGNNLSTKAKMYVPGLTRQVNECPFVLQVVELTGIPYITRYGAT
                     G"
     gene            4880..5293
                     /locus_tag="PLE23_08170"
     CDS             4880..5293
                     /locus_tag="PLE23_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83275.1"
                     /translation="MLKKILIGLLIAGAVAAGYGYYLFNLTEKSLTDVKADHSTDVTS
                     FLQEYESDETASDAKYLGKIVEVKGKLDNVRTDENGQVKISFESDNPMATVLAEMDQA
                     EKDKALKLTKGNEVTIRGKCTGKLSDIVCVNCVIK"
     gene            5297..5851
                     /locus_tag="PLE23_08175"
     CDS             5297..5851
                     /locus_tag="PLE23_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83276.1"
                     /translation="MQKIFLTIVLVFIASAGFAQLLHGPNGYMLVNLSSEKCKFWATT
                     TRSLSNINTQTGKFETVVYNESFVSANDTASNIFNDDYLNYKEFPELRLNGFVYNFDK
                     MDLSKEFRQTITLRGVLYMHGIKKDVEYTGNIYNRPQLMMLDYELYVDPNDFSLKAPA
                     NLASCISPLMLIKGNVQLVDMSKE"
     gene            complement(5928..6437)
                     /gene="azu"
                     /locus_tag="PLE23_08180"
     CDS             complement(5928..6437)
                     /gene="azu"
                     /locus_tag="PLE23_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HMX83277.1"
                     /translation="MKKSLFFFLSLSLFFASCGGSSEEAEQAEETTTEESAPAAAAPS
                     NEAMLAITGGDDMKYDLTELKATEGQTVKLTLTHSGKMPKESMGHNWVLLAAGTDVDA
                     FAKEAINAKDNDYIPAALASSVIAHTKTIGGGETTDVTFTAPAKGTYDFICTFPGHYA
                     MMRGKLIVE"
BASE COUNT         1877 a         1337 c         1328 g         1965 t
ORIGIN      
        1 taggtcgcgt ctcagtattc tgacatcatg ataagcagta aaaatacgcg tcgtgtcaac
       61 cgaatcccgt atcatcctaa atgcctttag cgtatcggat gtaaggaatg tagagtcggc
      121 accatcataa gatatcagca atggtctgcg gccaaatgct ttgagatagt cgttcgaacg
      181 gtcaaaaacg gttgtatcac attttgtaat aagcctggct gatgtatcct gccagatgac
      241 attgcccgag gccgtgccaa aaccttttgc cttgctgtaa tccataaaat cggcctccat
      301 aatctgcggc ggctcggaca ctcttgaacg tcccttggtc ttgaagctac cgtcattgcc
      361 gttgtagtgt atttcctccc ctgcaaaatc ctgtttacct tttctgtact tggcatttcc
      421 gtagagtgaa gtcagttttg actgttcatt gtgtacgatt cgctgagcgg atgccttttc
      481 agtagatgat tcgtacctgg cattttctac caaggagact tctgcaagat acccttcata
      541 cctaatggta tcagctgtgg cttttgtgtc tttcccttcg tattgtgcat tgtgggtaaa
      601 ttctgccttg cggttgggta tattataaaa tccggcttcg caataaatct tagctgtgtc
      661 ttgtactata agtgtaggtc ctttaaacac tgctgtgcgc cggtcggtga gaaattcgag
      721 tgaatcggct ttcagtgtaa aggtagaatc gacaatacgg acatcttttt gaaaaaaagc
      781 tttcttttca ttgaccgaat acatacctgt acggcttgac aattttgttt tgccgtcatt
      841 caagatggct ttgttatcgt atttggctat tttagtatca agattgtaat tgagtcttga
      901 agtaaacaat tgctgagtac cattgaccaa aacaacatca ccgaagagat cggcatcttt
      961 ggtatcacca ttataccata gggaatcaga aaacacatga atggtgtcag tctgttggat
     1021 gagaaccctg ccgtaggctt tgagctggtt attaagcagc aacgccgaat cgcacgacat
     1081 atacgtattg ccctgcctta gccttacttt gcctaccatc cactggtact cgagtccgtt
     1141 aatttttttg tagtaaaatg tttcagcttt ttctacaatg atgaatttgc cgcttattgt
     1201 atcttgctgt tgggcaaaaa gcaaatttga cataaaaaca ctcaagaaaa tagctataat
     1261 aatccgcata atttcactta acgattaaga gactacatct gttgtccggg ctgttgaaat
     1321 acctaacatc aactctgctg cttcatgacc tgccaatgta ccgagtgcca cacccattcc
     1381 gcccatccta actgctacta tcacgttttc tgatattttc tttatgatcg gaaattttac
     1441 tttgccaata ccaagaatac cagaccactg catatcgatg cccggaaatt cgtgtggtgt
     1501 tatccaggtt tttagcattt gcacaagatg ctcctgaatg tcgggattga tgctcagtga
     1561 atctgtgtat tccgtatctg gaaaaaggtt tcggccacca ccgagtagta ttctgccatc
     1621 tatttcgcgc cagtaaaaat atccaaggtc gtaatgaaag cacgaatcga gcttcatgtt
     1681 tttataggga tgcgtcacca gtacctgatt ccttgccgga aaaacttcca gctcgggtaa
     1741 gagctggcgt gcaaatccgt ttgtggctac aatcagctgc tttgcttcga cataatactc
     1801 tgactgaagg cgtagtgtga cttttgtcga ttcatcttca atctgtctga cttcggcacc
     1861 aaagtaaaag acaatacctg ctgtacgggc aagtgtaagg agtttttgca tcatcattcc
     1921 ggtatcgagc cggccttcac acttgttgag tataagatgg ctaacatccg agaaaccaaa
     1981 attctgaatc ttcttgtcgc agatgctgta agtatcgtcc acgccagtta tttctttaac
     2041 cagacggttg agccggtgaa tattatcatg acaatgcgag aaaaaatctt cttcatctgt
     2101 cttaaaaacc tcataattgc caccaggcct gaagaaaatt tcatcatcac ctgttcgctt
     2161 gcgcaatgct gccaaaccgg caaaccgcct tgctaccaga ttaagtgttg actcctcgcc
     2221 cattttatca aaatcatcaa gcaactccga cacgcttccg aaacaagcaa agcctgcatt
     2281 tttggtactt gcacctgaag gcaaaaaacc acgctcgatg atgagaatct tcattgaagg
     2341 atcatgctca atgagtgaaa gcgctgccga tatgcctacg atgcctgcac cgatgatgac
     2401 cgtatcgaaa tctgacataa aagacttgcg ctcccagtaa cttaactgca ttattttaca
     2461 gacttaaggg gtaaaagtac attgtttatg agagaataca aagcaggact tcgatttttc
     2521 aattaatcgg aatatatttg cacctcgtaa cagaactgtt ctactatgat acaaagaata
     2581 caatccgttt acttggtgtt agccgttatt gctctggcca ttttattttt cccaatatct
     2641 tttggtgact tgaaagcagg atctacaatt gctaaaacgc ccgctctctc agatggcaca
     2701 ctcaatctcg aggacaataa cctgtcgatg atatgtactt ttgccggtat tgccttgttt
     2761 atggcagcta tatttttatt taaaaacaga agattgcaaa ccagaatagt agcactaagc
     2821 atgttggttg ctttgattat attcatcctg gccggctggt tattcatgag tagtagtaag
     2881 atagctggca atgatttgtc tccgggcatt gggcttggtt cgccggtgat agccattttg
     2941 tttggctggc ttgccaaccg atcgatcaga aaagatgaaa accttgttcg gtctatggac
     3001 agacttcgtt aagaaaacct cagcaacact attaatgaaa aaaacaccac tgttcacact
     3061 tttacttaca atatttatag atattgtagg ttttagcatt attattccta tccttccggt
     3121 ttattcgaag gagattggtg ccagcaatat catggtaggg atcattgcag gcagttattc
     3181 actgatgaac tttttgtttt ctcctgtgtg gggaaatata agcgacagaa ttggacgcaa
     3241 acccgtgatc cttgtatcgt tgtttattac tgcctgttcg tatctgttgt ttgcatattc
     3301 gaagaccatt tcactcttgc tcatatccag aattatagcc ggtataggct ctgccaatta
     3361 tggagcggca caggcataca tctctgacat aacaaaaccc gaagaccgtg ccagagcatt
     3421 cggctatgtc ggtgcagcgt tcgggctcgg gttcatcatc gggccggtga ttggcgtatt
     3481 tatattaaaa atgtggaatg tagctgccat cggatatgta acaatggcgc tcaaccttgc
     3541 caatatgctg atggcaatgg caatattgca tgagacccgt aaagaagtga aatcaggcac
     3601 tccgttgtta gaaaatccga ttataaatgt acgcgacggg ctcaaaactc cggttttagg
     3661 taagttgatg tttattagcc tcctctacat aacggcattg tcgatgatgc agataaccat
     3721 ttcggttttt tggtctgaga agtacgggtt gagcaaaaca cagatatccg gaatgttcgc
     3781 ctttatgggc atcatgtcgg tactggtaca gggttttctg gtgggtaaac tcacacttaa
     3841 attcggagag aaaaacatga tgataatagg cgctgtatta ctagcagccg gactgtacat
     3901 aatacccatg gcgccttaca aagaaggatt ttatcttgta gaaggctttg ccattattct
     3961 tatttcattg gccaatggct gcctttcacc gagtataacg tccttgttat cacagaatgc
     4021 aggtgcagaa gaacaaggca aggtactggg tgtcggtatg tcagtcaata gtctgggcag
     4081 agtaatcgga ccatacgctg gcggattcct ttatggtatg gcaatgttta tgccgtactt
     4141 cataggcaca ctaatcatga taggtgcctg gaatgttgcc aggcgattca gaacaaaata
     4201 tgctcacata taattttgtt catgatgtaa atgccgcaat tcaacattca gtggtaccat
     4261 ttaattttta gcacataagg cataaattaa gcatatagcc tacgaaaatt cctgccattc
     4321 aatgaaaatc tctatattag accattgttt tccggtagcg ttgctacact gagcagtacc
     4381 ccctgcaatc agtccgtcaa aagatatggt tctgtgcgct gatgcggctc tcccctacac
     4441 attatccggt agcgccatat cgggtaatat atggaatacc ggtcaattcg accacttgca
     4501 acacaaaagg acactcattt acctgccttg taaggccggg cacatacatt tttgccttag
     4561 tcgaaagatt gttacccacg gtgtaggaaa attgatttgc gtgtaaatat tattgttttt
     4621 acaaagatca gactcccttt tatcaaccca gataacgcat taggaatata aacgaaaatg
     4681 atgattacat ataatccatg aaaaagtatt aaaagcaaat tgaactatgt tttttgagct
     4741 tttgaaatgg atttgcagta atatttttta cagtaaagag cctaatacgt tatctgggtc
     4801 aaactatttt gcggcacaat ttgttaaaca cctatatagt tttacattta ctgaacatcc
     4861 taaaacaaaa attaaacaaa tgctaaaaaa aattcttatt ggtctgctta ttgcaggtgc
     4921 agtggcagcc ggctatggtt attacctgtt taatcttact gaaaaaagcc ttacagatgt
     4981 aaaagcagac cactcgaccg atgtcacttc gtttttgcag gaatacgaat cagacgaaac
     5041 cgcctctgat gcaaaatacc ttggcaagat tgttgaagtt aaaggaaaat tagacaatgt
     5101 aagaaccgac gaaaatggtc aggtaaaaat aagttttgaa agcgataacc ctatggcaac
     5161 cgtacttgca gaaatggacc aggcagaaaa ggataaagca ttaaaactta ccaaaggtaa
     5221 cgaggtaacc atccgtggca aatgtaccgg aaaattgagc gacatcgttt gtgttaattg
     5281 tgtaataaaa taaagtatgc aaaaaatatt tttgacaatt gtactggtat tcattgcatc
     5341 tgcgggtttt gcacaattgc ttcatggccc caatggctat atgttggtta atttgtcctc
     5401 tgaaaaatgc aaattttggg ctactaccac aaggtctttg tcaaacatca atacccaaac
     5461 gggcaaattc gaaacagttg tctataatga atcattcgta agtgcaaacg atacggcttc
     5521 gaatatattt aatgatgact acctcaacta caaagaattt cctgaattgc ggctcaacgg
     5581 atttgtatat aacttcgata aaatggatct ttctaaggaa ttcagacaga ctattaccct
     5641 cagaggtgtc ctttatatgc atggcattaa aaaagatgta gaatatacgg gcaatattta
     5701 taaccggccc cagctcatga tgttagatta tgaattgtat gttgacccaa atgactttag
     5761 cctcaaagca cctgcgaatc tggcttcctg catttcgcca cttatgctca taaaaggaaa
     5821 tgtgcagtta gttgatatgt cgaaagaata aaaaacagca gacaatatat tcaaatactg
     5881 tctgctcatt accaaatgtt aaaaaatatt tcgaagaagt ctgtgaatta ttctacgatt
     5941 aatttgcctc tcatcatagc atagtgacca gggaatgtac atatgaaatc ataagtacct
     6001 ttggcaggtg ctgtaaatgt aacgtctgta gtctcgccac cgccaatggt cttggtgtgt
     6061 gcaataacag aagaagctaa tgcggcagga atgtagtcgt tatctttggc attgattgcc
     6121 tcctttgcaa atgcgtcaac atctgtaccg gctgccagaa gcacccagtt gtgacccata
     6181 ctttctttag gcattttgcc actgtgggta agcgtaagct taactgtctg accttctgta
     6241 gccttcaact ctgtaaggtc atacttcata tcatcaccac ctgtgatggc aagcattgct
     6301 tcgttgctag gtgcagctgc tgcaggagct gattcttcag ttgttgtttc ttcagcttgc
     6361 tctgcttctt cactagagcc tccgcatgat gcaaaaaata atgaaagaga aagaaagaaa
     6421 aagagtgatt ttttcatttg ttattaaatt ttaaaatgtg agaaaataaa aattagttaa
     6481 ttgtttgcaa gtgattcttc aagttct
//
LOCUS       DAOQQY010000155         6478 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5065_length_6478_cov_5.826094, whole genome shotgun sequence.
ACCESSION   DAOQQY010000155 DAOQQY010000000
VERSION     DAOQQY010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5065_length_6478_cov_5.826094"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLE23_08185"
     CDS             <1..383
                     /locus_tag="PLE23_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HMX83278.1"
                     /translation="DTTAIINWTNGNTGESYIDAIMKELNATGFISYKCRQLAAAFFI
                     YELKQNWQTGAEYFESRLIDYDVCSNWVNWNYIAGLGIQRIESKGLNVGQLAQKYDPD
                     SRYLDCWNHPGSLKKFHPGMLEGK"
     gene            494..1525
                     /locus_tag="PLE23_08190"
     CDS             494..1525
                     /locus_tag="PLE23_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMX83279.1"
                     /translation="MRIFLLSTLILLFGVTIKAQDRHFSQFYSAPIYLNPALTGNYDG
                     GFRIGLNYRDQWRGTLDNPFVTYSGFVDLKMKARLSAAQKDYAAVGAIFFADQVSAVD
                     FNTNNMSFTGALHKSLDINNKQYLSIGFQFGISSRNVNYEKLNFADQFNGVDGFTENT
                     NELLPANNFSFGDLSTGLHYSIRIKKHASLYAGLALHHILQPAISFYEKPTEEIYLQR
                     KFTLYGGSELPISTSTSLNPRIFVMKQGEHLEMTGGANFRTLVNDYSGVAVHFGGWLR
                     TNRSITGPVNPDAVILLAGFEYNRILFGVSYDINLGSLSKSRGRQAFEFSLGYLGETE
                     EDGIVCPKF"
     gene            complement(1528..2142)
                     /locus_tag="PLE23_08195"
     CDS             complement(1528..2142)
                     /locus_tag="PLE23_08195"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HMX83280.1"
                     /translation="MKKTLLILFVLSVVACKKPGKIVDNNNSTNTSSVTDSSATAATP
                     TAVQPMKTDEIKIGFVMADTVLEKFSYYQKVKASLTAKGTKLEKDMEARALAIQKEYQ
                     GYQQRGQSMSPKELQEAEMSLMKKQQDLEDFRTKQTAALMKEEEALNKDLQKKIEKYL
                     DTYAAENGYTFILSKHIGGSLLFGKKELDITKDVINGLNASIKK"
     gene            complement(2206..2622)
                     /locus_tag="PLE23_08200"
     CDS             complement(2206..2622)
                     /locus_tag="PLE23_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HMX83281.1"
                     /translation="MENNLLELSKDGKKISSVLPPHVKNFLIDIDGTICEDIPNEEPE
                     RMADAFIYPGALEKINQWYDDGHIITFFTSRTESHREVTTFWLNKHGFKYHGLLMNKP
                     RGGNYHWIDNHDVLATKYTGRFTEFVEKMTPTQVFE"
     gene            2896..4986
                     /locus_tag="PLE23_08205"
     CDS             2896..4986
                     /locus_tag="PLE23_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HMX83282.1"
                     /translation="MLRQVFNYFRYPFYSQKDLSDCGIEALRMVLAFHKLAHDKARVD
                     ARFELDDFGTNFLAIQNAANELGLDCIAAKVALAEMSHLPLPVIIHWNNHHFIVVYEI
                     RERSVLVADPASGMREISIAEFAENCFNGDNLQVPKANVLIFEKNNHVKQKKQAHLKT
                     TSMIGVKTWVSALTTTISVTYILLSLLAFTCSGMMPGHDMLLLGGAIILFFLSVIFIK
                     SFSYGKNIYSGQNLSSDILQYAEKSESIDYQVDKIINAFYLGQYLKVNKPLAIIAWSV
                     VAGIMLFGFAHSGLIGASFFALALLYFLVLKGYTKHFENKELNRVANAEAMHRQLLNY
                     MHKSAEFRKVGKRNTDGLTVDAGITPESFTRPEKHSWLTGLFLLSGFVLLFRSYHYSE
                     IGFCRLIWLAATFVMMILAIDYTNTFHWINRKWFRLQFGNSNATDAGAKLYATGVTAD
                     LRTDVSVQNIACHYPSRNFDSGLVNIHFFIPAGSKLAIIGRQGSGKSTLIKLMTGQIK
                     PDSGLLTIGGIRMEDIGTEQFLDITGICTNNSELLEGTVEWNITLEKETKNNKRLKDV
                     IDATMLNNFIINVEDGIKLFVEPSRNNISDSIRRKILLARLLYQNKPLIFIDLEDQFT
                     DPIENLTLFESVMDYCHDKTVVYTTKNTDVAGLASQIVLLENGEQLMVKTGAEYLKMN
                     ERNELTTTDQENRH"
     gene            5061..6032
                     /locus_tag="PLE23_08210"
     CDS             5061..6032
                     /locus_tag="PLE23_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83283.1"
                     /translation="MDNAYFVQTSSYEPINDTAFIHKLQYALLSDGVKVEKSQLLGYS
                     KSREEVEALTALNEGLKNFSDANDLKQRFKNNSIKDYEINELLTSLIKLDGTQALSKP
                     KLRTEKIRRRIATNKDEKVRLAEKALHQAQQAYAQRKISISELKNASDILADARSMPE
                     YREVITTITQKLPANATGQKLDKETQQLLLSKIQERIRAWFASAEVTAEVSGIFYTNQ
                     SGKAFIVQKHTQGTLQFFDTSLSAETVAVNILCDGGEELSGTARRSSEPGYYKISLND
                     SSAARCIDSVDSKVVIKVYPEKSTLFTRLMNTVRSDKNSTKAKAPAH"
BASE COUNT         1977 a         1154 c         1324 g         2023 t
ORIGIN      
        1 atgatacgac tgcaatcatc aattggacaa acgggaatac cggtgagtca tacatcgatg
       61 ccatcatgaa agaactcaat gccacaggtt ttatatcata caaatgtcgc cagctggctg
      121 ctgctttttt tatctatgag cttaaacaaa actggcagac cggagctgaa tattttgaaa
      181 gtcgccttat tgattatgat gtgtgtagca attgggttaa ctggaattac attgcaggct
      241 taggcattca aagaattgaa agcaaaggct tgaatgtagg acaactggca caaaaatatg
      301 acccggatag ccgatacctc gattgctgga accatcccgg ttcattgaag aagttccacc
      361 cggggatgct tgaaggaaaa taacgctaca ttttattttg cccaagaggc ttatttacag
      421 ataaaacata aagtgtatat ttgcgttaaa agctatcgaa gataagtagt catcatttac
      481 ttaattaagc cacatgcgga tttttttgct cagcacactc attttgttgt ttggtgtaac
      541 catcaaggca caggaccggc atttttcaca gttttactca gcacctattt atctcaatcc
      601 tgcacttacg ggtaactatg acggtggctt cagaataggg ctcaattacc gagaccagtg
      661 gcgtggcact cttgataatc cttttgtcac ctacagtggt tttgtggatt tgaaaatgaa
      721 ggcaagacta agtgcagcac aaaaagatta tgctgctgtg ggtgctatct tttttgctga
      781 tcaggtgagt gctgttgact ttaataccaa caatatgtca ttcacaggag ccttgcataa
      841 gtcgctggat atcaataata aacaatatct atctataggc tttcagttcg gcatttcttc
      901 aagaaatgtc aattacgaaa agttgaactt tgctgaccag tttaatggtg ttgacggatt
      961 tacagaaaat accaatgagc tgcttccagc caataatttt tcatttggag atttgtcaac
     1021 cgggctgcat tattctatcc gtattaaaaa acacgcttca ctttatgcag ggcttgcctt
     1081 gcaccacatt ctgcaacctg ccatttcatt ttatgaaaag cccaccgaag aaatctatct
     1141 ccagagaaaa tttacattat atggtggctc agaacttccc atttcaactt ctacaagtct
     1201 caatccaaga atttttgtaa tgaaacaagg cgaacatctt gaaatgaccg gaggggcgaa
     1261 ttttagaaca ctcgtcaacg actacagtgg cgttgctgtt cattttggtg gctggttacg
     1321 taccaatcgt tccattaccg ggcctgtcaa tccggatgct gtaatacttc ttgcaggctt
     1381 tgaatacaac agaatacttt ttggcgtatc ttatgatatt aaccttggca gcctgagcaa
     1441 atcgcgtggc agacaggcat ttgaattttc acttggctac cttggcgaaa ctgaagaaga
     1501 cggaattgtg tgtcctaaat tctgaaatta tttctttata gaagcattca ggccattgat
     1561 gacatctttt gtaatgtcaa gttctttttt accgaaaagt aagcttccgc caatatgttt
     1621 tgaaagaatg aatgtatatc cattctctgc agcataagtg tcaagatatt tttctatctt
     1681 tttctggaga tccttgttga gggcttcttc ttccttcata agtgcagctg tttgtttggt
     1741 gcggaagtct tcaagatcct gttgtttttt cataaggctc atttcagctt cctgcaattc
     1801 tttaggtgac atactctgac cgcgttgttg ataaccctgg tattcttttt gtatggcaag
     1861 tgcgcgtgcc tccatatctt tttcgagctt cgttcctttg gccgtcagac ttgctttcac
     1921 tttctgataa taagaaaatt tttcgagcac tgtatcggcc attacaaagc ctattttgat
     1981 ttcgtctgtt ttcatcggtt gaacagcagt aggtgtggca gcagttgctg aactgtccgt
     2041 aactgatgaa gtgtttgtgg aattgttgtt gtcaacaatc tttcctggtt ttttacaagc
     2101 gacaacagac agtacaaaaa gaataagaag agtctttttc atgaatttat tgattaaatt
     2161 ggaccgcaaa tctatggaaa aaattaaagt gcattagtaa aaaaattatt caaaaacctg
     2221 tgtaggggtc attttttcaa caaattcggt aaaccggccg gtatatttgg ttgccaacac
     2281 atcgtggttg tctatccaat gataattgcc acctctcggt ttgttcatca acagtccgtg
     2341 atatttgaat ccgtgtttgt ttaaccaaaa agtggtcact tcgcggtgtg attcggtacg
     2401 tgatgtaaaa aaagttatga tgtgcccatc gtcataccat tgattaattt tttcaagtgc
     2461 accaggataa ataaacgcat cagccatacg ttcaggttct tcatttggta tatcttcgca
     2521 gatggtacca tcaatgtcga tgaggaaatt cttaacatgt ggcggaagaa cggagcttat
     2581 ttttttaccg tctttgctga gttccaaaag gttattttcc atgatttgta tgttgttctt
     2641 ttggtatgac accattgaag ggtgcaaagt tcctcaaata attgaaaaat caaatatccg
     2701 gaacaactta tttgaatgct tcatgaggaa tttgtttagg gcaaataatt ttgattgcgt
     2761 tgaagattgt tcattctgta gtattttctt gctttttgta taaattaaat gtttgttgaa
     2821 atgtttgtaa tgcattgtta tttaaatatt tatatatgtt ataaaaacgg tatgattgtt
     2881 gtatgatatt aattgatgtt aaggcaggta tttaattatt ttcgttatcc tttttatagc
     2941 caaaaggatt tgtcagactg tggcatcgag gcgctgcgga tggtactggc atttcacaag
     3001 ctggcacatg ataaagcacg agttgatgct cggttcgaac tcgatgattt tggcaccaat
     3061 tttctggcaa ttcaaaatgc agccaatgaa ctcggactcg attgtattgc tgcaaaggtt
     3121 gctttggctg aaatgagtca cttgccattg cctgtcatca tccattggaa taatcatcac
     3181 ttcatagttg tctatgaaat aagagaaagg agtgtactgg tggcagaccc tgcaagcggt
     3241 atgcgagaaa taagtattgc ggagtttgca gagaactgtt ttaatggcga taatcttcaa
     3301 gtgcccaagg caaatgtact gatcttcgaa aaaaataatc atgttaagca aaaaaaacaa
     3361 gctcatctga agacgacatc tatgatagga gtcaaaacgt gggtatccgc tttaacaact
     3421 acaatttcag ttacttatat acttttaagt ttgctggcat ttacttgttc aggcatgatg
     3481 cccggccacg acatgcttct tctgggtgga gccattattc tctttttctt gagtgtcata
     3541 tttataaaat cattttctta tggaaaaaac atatactccg gacaaaatct ctcgagtgat
     3601 atattgcaat atgccgaaaa atcagaatct attgattacc aggttgataa aataattaat
     3661 gcattttatc ttggtcaata tttaaaagtc aataagccat tggctatcat tgcttggtcg
     3721 gtcgttgccg gcattatgct gtttgggttt gcgcacagcg gcttgattgg tgcctcattt
     3781 tttgctttag ctttgttgta ttttcttgta ctcaagggat atacaaaaca ttttgaaaat
     3841 aaagagttaa atagagtagc caatgctgag gctatgcaca gacaattgtt gaattatatg
     3901 cataagtcag ctgaatttcg caaggtagga aagagaaata ctgacggatt gaccgtcgat
     3961 gcaggcatca ctccagagtc tttcaccaga cccgaaaaac atagctggct gacaggtttg
     4021 tttttattat ccggattcgt tttgctgttt cggtcttatc attattccga aataggcttt
     4081 tgcagattga tttggctggc agcaacattt gttatgatga ttctggcgat tgactataca
     4141 aatacttttc attggattaa cagaaagtgg tttcgattgc aattcgggaa cagtaatgca
     4201 actgatgccg gtgctaagtt atatgctact ggtgtcacag cagatctcag aactgatgta
     4261 agtgtgcaaa atatagcctg tcattatcct agcagaaatt tcgattcggg cctggttaac
     4321 attcatttct tcatacctgc gggttcaaaa ctcgccatta ttggcagaca gggcagtgga
     4381 aaatctacat taatcaagct tatgaccgga cagatcaagc cggatagcgg attgctaacg
     4441 attggcggaa tccgaatgga agacataggt actgagcaat tcctggatat caccggcatt
     4501 tgtacgaaca acagcgaact tcttgaaggt actgtagagt ggaacattac tttagaaaag
     4561 gaaacaaaaa acaacaaaag acttaaagat gtcattgatg caacaatgct taataatttt
     4621 attatcaatg tagaagatgg tataaaattg tttgtggagc cttcgcgaaa taatatttct
     4681 gacagtataa gacgaaaaat cttactcgca cggcttttat atcaaaataa gccactaata
     4741 tttattgacc ttgaagatca gtttacagac ccgatagaga atctgacctt gttcgaatcc
     4801 gtaatggact attgtcatga taaaactgta gtgtacacaa cgaagaatac agatgttgcc
     4861 ggtctggcca gccagattgt tttgttagaa aatggagaac aattgatggt gaaaaccgga
     4921 gcagaatact tgaaaatgaa tgaaagaaac gaattgacca caacagacca ggaaaatagg
     4981 cattgaaaaa tattaatata tattggatat tgttcttagt tggattttgc gtctggctgg
     5041 caactgtatt atttgtacgg atggacaatg cttactttgt acaaacatcg agttatgaac
     5101 caataaatga taccgcattt atccataagc tacaatatgc tttattgtca gatggtgtga
     5161 aagttgaaaa atcgcagcta cttgggtaca gcaagagtag agaagaagtt gaagcgttaa
     5221 ccgcactgaa tgaaggtttg aagaattttt cagatgcgaa tgatttaaag caaagattta
     5281 aaaacaattc gataaaagac tatgaaatca atgaattact aacctctttg ataaagcttg
     5341 atggtactca ggcactctct aagccaaagt taaggacaga gaagataaga aggaggattg
     5401 ctacaaacaa ggatgaaaaa gtacggttgg ctgaaaaagc actacatcaa gcccagcagg
     5461 cttacgctca gaggaaaatt agcatcagtg aattaaagaa tgcatcagat attctagcag
     5521 acgccagatc aatgcctgaa taccgtgaag tcattactac cataacgcaa aagttacctg
     5581 ccaatgcaac cgggcagaaa ttggataaag aaacgcagca attattactg agcaagattc
     5641 aggagcgcat ccgtgcatgg tttgcttcgg ctgaagttac agcagaggtg agtggcattt
     5701 tttataccaa tcaatctggc aaggccttca ttgttcaaaa acacacacaa ggcactttac
     5761 aatttttcga cacttcactc agtgccgaaa ctgttgcagt gaatatactt tgtgacggtg
     5821 gcgaagaatt atcaggtact gcaagaagat cttcagagcc gggttattac aagatttcgc
     5881 tgaatgattc ctcagctgcc agatgcatcg attcggtcga ttcgaaagtc gtgataaaag
     5941 tatatcccga aaagagcaca ctattcaccc gcttgatgaa cacagtcaga tcagacaaaa
     6001 actccaccaa agccaaggct cctgcacatt aagtaatgct tcctttatta taatgagagc
     6061 atcatttact ttttaaagga aggtaaattt ttccgctgca atgcgtcact aaagtattta
     6121 gttgaaaatt gttgttctgg tgagtatttc agaaatcaca gtagtatgtt atcaggatga
     6181 tatttctaag ttttattgat ttctgggttt taaagaattt ggtgtaaaag acatattttg
     6241 atcgcttgcc accatcaaac gccttgtagt catccattta ttctgccaat tggaaacaaa
     6301 aggtaggaaa ctccatacag tgaaagtaca aattgtaatg taaaaatttt ttattattta
     6361 ataaatggga tatagtaaaa cttttacatg caatgttcaa ttgccatgtc acaaaagttt
     6421 atatcaaaat gtgagaaaat ccttgttttt cttcgcggga tattgacatg atataact
//
LOCUS       DAOQQY010000156         6445 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5114_length_6445_cov_5.142097, whole genome shotgun sequence.
ACCESSION   DAOQQY010000156 DAOQQY010000000
VERSION     DAOQQY010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5114_length_6445_cov_5.142097"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..2151)
                     /locus_tag="PLE23_08215"
     CDS             complement(202..2151)
                     /locus_tag="PLE23_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HMX83284.1"
                     /translation="MENSRIVQKGKDLTKGHQWFQKFSTFFSRIYSTELNPMYNLGGI
                     SVLLFAIACISGIYVFIFYNINPRHAWDSVEAMSNNVFNGWMRSIHRYSSDLLVIFIL
                     IHLLHTLLTSKFKRLVSWVSGIISFLVVILIGVTGFMLVWDQKAKLTGYLTAKLFSSL
                     PIFDPSIAGAFLLNDLDVVGGFFKVALFGHIVFSLVTIIIIWVHVLRISKPKIFPPKK
                     LIYYVIIALGIIAIAFPVKSDPPAQASNLPISTTFDWYYYFGYYLMKLFSVNTNWLIL
                     IGSGVVLSIIPYLIKRKNNPPAFIDLDKCNACNLCAYDCPYEAIDMLQVEGKRKAILD
                     PDKCVGCAICIGSCDEHAITHPMFPDLVVMPKPKSDVTVFSCSYFPEPELPSELNIQR
                     YRVPCTGSIMPKDVQRMLENNTEKVAILSCEDCYYRLGKTWTINRFLRKRAPLFSKKF
                     DASLVQLLTLTQYSKEKLLAFSKETVSEEGGSGEINIGDHKKGNPVLSVLIMTLFFAL
                     MIPLSSTTVRFFNPAEKTLIVNFKYISSPTEYEQFGSGAAHMQVKNPAVKRRSPVTLK
                     IFSTKDNKLIFEKEYAPRGLRHDIAMFIFTQMVVDEDAVDVVLTETAFPDKQYKLDNI
                     KLKQGDGTFVILRDDKLIVADKQGF"
     gene            complement(2153..2887)
                     /locus_tag="PLE23_08220"
     CDS             complement(2153..2887)
                     /locus_tag="PLE23_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMX83285.1"
                     /translation="MNRIILPAIVWMLLCGFNLPVEKDFLGQKAADIPVFNAKGDTFP
                     FFSMLGEKSLIICPVYTKCKTYCGLISNGIHKAIKESGGLSKDFRVVSFSFDSTDTAE
                     DLDFYNQLYRMDGVNWQTISASQEHIAQMMSSIGFEYEYDPNTKEFNHPSYLVVLTPS
                     GRISRFIYGLEPSKKDIELAGIEAMSEKVRPGLFRGFYLRCFGYDPVLKTYKVDWRFI
                     ISTSAGLLIVSLVSSIFIKSFIVTKE"
     gene            complement(2974..4641)
                     /locus_tag="PLE23_08225"
     CDS             complement(2974..4641)
                     /locus_tag="PLE23_08225"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMX83286.1"
                     /translation="MFRTCDITGFKVDLQSEKLIRYNAVVSVVSLLLAVTAALLLVFT
                     RYQPVHLLNAEWYYRLVTFHGLNALIFWIIFFEIAGLYFGSTVLLNTKFCSPRWGWIA
                     FILMVAGLVMSNVIILMGKADVMLTSYTPLKAHPLYYLGIILFAVGALIAVALFFGNL
                     MIARRDNTYGQSLPLVVYGLMTAAIIAVITLASGAIIYIPTFLWSLGLVQNIDPAMYK
                     LIWWGLGHSSQQINVAAMVSIWYMTSFLTVGGTSINERVSRSAFVLYILFICLASAHH
                     LLTDPGVSSAWKVWNTSYAMYLAVLASMIHAFAVPSSFESAQRRWGYNKGLFQWLSKA
                     PWGNPGFSAIILAIIGFGFIGGTTGVIFGMEQTNIIVHNTIAIPGHFKGTVVIGTTLT
                     FMGITYYLIPLIFRRKIVGFSWAKIQPWLFFIGIALLSISMIVLGQLGVPRRHWDSTF
                     SGGPFTYNFNPAVDFFWALMMLGGVIAFIATLIWILIVVISVFWGPKVNGPQDMQLTI
                     SPLAPAAKEHKGYEAPGTLVLTMIFLLVFLALFFLNWKWLAATWNVN"
     gene            complement(4660..5583)
                     /locus_tag="PLE23_08230"
     CDS             complement(4660..5583)
                     /locus_tag="PLE23_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMX83287.1"
                     /translation="MSLLSPAEGWYDKKVSKDEKMWMLIALILCLSMFVWMILWHVYG
                     KQNPSSTTYRTSTAEFSTLTEAYIKKNQIGSDNGIPIVRPKPNSDVFLMAEMWRWSPG
                     LILEKDQKYKFHISSRDIVHGFSLQPVNMNFQVYPGYDYVLEFTPTELGEYKLLCNEF
                     CGIGHHTMIGKIVVIEKDEDLKKYGYEGFKKEPDPVPAAGVVANLTEEQKASSGEQLY
                     TLKGCAACHKVDGTNVLAPSWKGLFGKEEKVTENGKEMSVKVDEAYIIESIKNPKQKV
                     TVGYEKTPMPVFPLKDDEIDHIIAYIKSLSK"
     gene            complement(5609..5794)
                     /locus_tag="PLE23_08235"
     CDS             complement(5609..5794)
                     /locus_tag="PLE23_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83288.1"
                     /translation="MENDKQPEEKKIPFSRMIMDDYMLLMFLGVTIYAIFYLLWGVME
                     LSNLPAIPEDIKNALLK"
     gene            complement(5925..>6445)
                     /locus_tag="PLE23_08240"
     CDS             complement(5925..>6445)
                     /locus_tag="PLE23_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMX83289.1"
                     /translation="STIDKPIILRVVSKGAILGHRENLKNGLHTNSATATTDVQYCFI
                     PYHLFSEIVKKSPVLKEQIINELLEELETTEKKAAILAHKSVREKVAEVLLLLARTYG
                     YEERPYSFEINFSRQDLADLIATTKEQVSKTMKDFEKEGIIKISGKKFTYLNLPVIKN
                     ICGCISLPKDTL"
BASE COUNT         1891 a         1406 c         1365 g         1783 t
ORIGIN      
        1 aaaaatttcc tgactctacc ttcggctaca tagacatgat cagcgacccc gaaaccggct
       61 atctctacac cggcgacggc gtgtacatga tgtgcctgaa accgccgaaa tgatggtgtg
      121 aaggacaaca aaaccggcat ttcaccgctt tggaaggaat gtcggttttg tttttttgaa
      181 caatctcgga cagtatgccc gtcagaaacc ctgtttatca gcaacaatca gtttatcgtc
      241 cctgaggatt acgaaggttc cgtctccttg cttgagcttg atgttatcga gtttgtattg
      301 tttgtcagga aatgctgttt ctgtcaaaac gacatccacc gcatcttcat ccactaccat
      361 ttgtgtaaaa ataaacattg caatatcgtg gcgaaggcct cgcggcgcgt attccttttc
      421 gaaaatcagc ttattgtctt tggttgaaaa tatcttcagc gtcacaggac tccttctctt
      481 cacggccgga tttttcacct gcatgtgtgc cgcaccggag ccaaattgtt cgtattccgt
      541 tggtgatgaa atatacttga agttgacaat caacgtcttt tcggcaggat tgaagaagcg
      601 caccgtcgta ctgctcaatg gtatcatcag cgcaaagaac aaagtcataa tcaatactga
      661 caataccgga ttgccttttt tatggtcgcc aatgttgatt tctcccgaac caccttcttc
      721 cgatacggtt tctttggaga atgccagcag tttttctttg ctgtattgcg tcaatgtgag
      781 caactgaacc agtgaggcat cgaatttctt agaaaataaa ggtgcccgtt ttctcaggaa
      841 tctgttgatg gtccaggttt tcccgagacg ataataacaa tcctcgcaac tcaggatggc
      901 aactttctcc gtattatttt cgagcattcg ctgaacatct ttcggcataa tactacctgt
      961 acatggcacg cgataccgct ggatattcaa ctctgacggt aattctggtt ccggaaaata
     1021 gctgcagctg aataccgtca cgtcgctctt cggcttcggc ataaccacca aatccggaaa
     1081 cattgggtgc gtaatggcat gttcatcaca cgatccgata cagatggcac agccgacaca
     1141 tttgtcgggg tcgagtatgg ccttgcgctt gccctccacc tgcagcatat ctattgcctc
     1201 atacgggcag tcatacgcac acagattgca ggcattacac ttgtcgagat ctataaaggc
     1261 aggcggattg ttttttcttt taatcaagta aggtatgatg ctcaatacta cacccgaacc
     1321 gatgagtatg agccagttgg tattgacgct gaataatttc atcagataat agccaaaata
     1381 ataataccag tcgaatgtag tactgatcgg gaggttcgat gcctgtgcag gagggtcact
     1441 cttaacaggg aaggctatag caatgatacc aagtgcaatg atgacatagt agatgagctt
     1501 tttgggcgga aatatctttg gctttgaaat gcgcaataca tgtacccaga tgatgatgat
     1561 tgtcaccagc gagaaaacga tatgaccgaa caaggcaacc ttgaaaaaac cacctactac
     1621 atcgaggtcg ttgagtagaa atgccccggc aattgatggg tcaaaaatag gtaggcttga
     1681 aaagagtttg gcagtcagat aaccggtcag ttttgccttt tggtcccata ccagcataaa
     1741 tccggtaaca ccgatgagta tcaccaccag aaatgaaatg atgccactaa cccacgatac
     1801 gagccgttta aactttgaag tgagcaaagt atgcagcaag tgaatcagga tgaaaatcac
     1861 caacagatcg gacgaatacc ggtggatgct cctcatccag ccgttgaaca cgttattcga
     1921 cattgcttcg acagaatccc aggcatggcg tggattgatg ttgtaaaaaa taaatacata
     1981 tatgcccgaa atgcacgcaa tagcaaacaa aagtaccgag atgccaccca aattatacat
     2041 cgggttgagt tcggtagaat atatccggct gaagaatgta gagaatttct ggaaccattg
     2101 atgacctttt gttaggtctt ttcctttttg tactatcctg gagttttcca ttttactctt
     2161 ttgtaacaat aaatgattta ataaaaatac tgctcacaag gctaacaatg agcagtccgg
     2221 ctgaagttga gataataaaa cgccagtcaa ctttgtaggt cttcagtacc gggtcgtagc
     2281 cgaagcacct gaggtagaat ccgcggaata gtcccggcct cactttttct gacatagctt
     2341 cgatgccggc tagttcaata tcctttttgg aaggttcaag accataaata aatcttgata
     2401 tccgaccgct aggtgtaagc accaccaggt acgatgggtg gttaaattct ttagtgtttg
     2461 ggtcatattc gtattcgaaa ccgatggaag acatcatctg tgctatgtgt tcctgcgatg
     2521 cagaaatggt ctgccagttg acaccatcca tacggtaaag ttggttgtaa aaatcaaggt
     2581 cttctgccgt atcggtgcta tcaaacgaaa aacttactac cctaaaatct ttactcagcc
     2641 ctcccgattc ttttatagct ttatgaatgc cgttgctgat aagcccgcaa taggttttgc
     2701 acttggtata gacagggcag atgatgagcg atttttcacc cagcatcgaa aaaaagggga
     2761 atgtatcgcc cttagcatta aagacaggga tatcggctgc tttttgtccg agaaaatctt
     2821 tttcaacagg cagattaaaa ccacacaaca acatccacac tatagccggc aatattatcc
     2881 tgttcatgat tgaaacttta tgataaatcg gggacgaacc ccgatttatc atattgattg
     2941 aaaacaaact aaccaacaac tacaatttat gaattagttg acattccagg tagcggccag
     3001 ccacttccag ttaaggaaga agagtgcaag gaataccagc aggaatatca tggtaagtac
     3061 cagtgtaccc ggtgcttcgt aacccttatg ctctttggca gcaggagcca gaggtgaaat
     3121 ggtcagctgc atatcctgag gcccattgac cttaggtccc caaaagacag aaattaccac
     3181 aatcagtatc catatcaatg ttgcgatgaa tgctatcaca ccgcccagca tcatcagtgc
     3241 ccaaaagaag tcaactgcag gattgaagtt ataggtaaac ggaccacctg agaaagttga
     3301 gtcccagtga cggcgaggca caccaagctg cccgaggaca atcatggata tggacaacag
     3361 cgcaataccg atgaagaaga gccacggctg aatcttagcc cagctgaaac caacaatctt
     3421 tcgtctgaaa ataagcggaa tcagataata ggtgataccc atgaaggtaa gtgtagtacc
     3481 gataacgact gtccctttga agtggcctgg tatggcgatt gtattgtgta cgatgatatt
     3541 ggtctgctcc atgccaaaga tgacacctgt tgtaccacct ataaagccaa agccaatgat
     3601 ggccaaaatg atggcagaaa aaccaggatt gccccatggc gccttagaca accattggaa
     3661 caagcccttg ttgtaacccc aacgacgctg agccgattcg aatgaagaag gaactgcaaa
     3721 tgcgtgaatc atagaagcaa gcacggcaag atacattgcg taactggtat tccacacctt
     3781 ccatgcacta ctcacacccg ggtcagtaag caggtggtgg gcagatgcaa gacagatgaa
     3841 taaaatgtac agtacgaatg ccgaacgcga cacacgctca ttgatagatg taccaccaac
     3901 cgtaaggaat gaagtcatat accagataga caccatagcc gctacgttga tttgctgcga
     3961 agagtgtccg agaccccacc agatgagttt gtacatggca gggtcaatat tctgaaccaa
     4021 cccgagcgac cacaggaatg tcgggatata aatgatggca ccagacgcaa gagtgataac
     4081 agcaataata gccgctgtca tcaggccata tactaccaga ggtaaacttt ggccgtaagt
     4141 attgtcgcgc cgtgctatca tcagattgcc aaaaaacaga gccacagcaa tgagtgcacc
     4201 tacagcaaaa agaatgatac ccagataata taaggggtga gctttaagag gagtatagga
     4261 tgtaagcatc acgtctgctt tacccatcag tataatgaca ttagacatta caagacctgc
     4321 caccataagt ataaaggcaa tccaacccca gcgtggagag cagaatttgg tattgagcaa
     4381 tacggtggac ccaaaataca accccgctat ttcaaagaaa ataatccaga atataagcgc
     4441 gttaagacca tgaaaagtaa caagcctgta ataccactca gcattgagca ggtgcaccgg
     4501 ctgataacgt gtaaatacta atagtagtgc tgcggttact gccagcagta aagataccac
     4561 tgaaaccacc gcattgtaac ggatgagctt ttcagattga agatcaacct taaatcctgt
     4621 tatatcgcat gttcggaaca tattatctga tttttctggt tattttgata atgatttgat
     4681 ataggcaatg atgtgatcga tttcatcatc cttgagcggg aatacaggca taggcgtttt
     4741 ttcgtatcct accgtcactt tctgcttagg atttttgatt gattcgatta tataagcctc
     4801 atcaactttt acactcattt ctttaccgtt ttcggtaact ttttcttctt tgccgaataa
     4861 tcctttccaa gacggtgcca atacatttgt accgtccact ttatgacagg cagcacaacc
     4921 tttgagcgta taaagctgtt cgccacttga ggctttctgt tcttcggtaa gattggctac
     4981 tacacctgca gcaggtaccg gatcgggttc ttttttgaat ccttcataac catatttttt
     5041 taggtcttcg tctttttcga tgacaacaat tttgccgatc atcgtatggt ggcctattcc
     5101 gcaaaattcg ttacacaaca atttgtattc gcccagttcg gtaggtgtaa attcgagcac
     5161 atagtcgtat cccggatata cctgaaaatt catattgaca ggttgtaaag aaaatccatg
     5221 aacaatatct cttgatgaaa tgtgaaactt atatttctga tctttttcaa ggataagacc
     5281 cggactccat ctccacattt cggccatcaa gaatacatcg ctgttaggct taggacggac
     5341 aatcgggata ccgttgtctg aaccaatctg atttttcttt atataggctt ctgtgagagt
     5401 agagaactcg gctgtgcttg tccggtaagt agtacttgaa ggattctgct tgccgtacac
     5461 gtgccacaga atcatccaaa caaacattga caaacagaga ataagtgcta ttagcatcca
     5521 cattttctcg tcttttgata ctttcttgtc gtaccatcct tcggcagggc ttagtaagct
     5581 catacatttg tttttttagg tgaaaaactt atttcaacaa ggcattctta atatcttccg
     5641 gtattgccgg cagatttgaa agttccatga cgccccacag gagataaaaa atagcgtaga
     5701 ttgtcacgcc gaggaacatc agcaacatgt aatcgtccat aatcattctt gaaaatggaa
     5761 tcttcttttc ttcaggttgt ttatcatttt ccataatgaa ttgctttagt tttacaatta
     5821 accatcgcaa acaaaactaa aggcacaaat tttatcctga aagaaagtgg cagaattaat
     5881 gttgcaaaaa atacgttttc gtactttttt gtgctaaata aatgttaaag tgtatcctta
     5941 ggcaaggaaa tacagccaca aatgttttta ataacgggaa ggtttaggta ggtaaatttc
     6001 tttcctgaaa tttttataat tccttctttt tcaaagtctt tcatagtctt tgacacctgc
     6061 tccttggtag tagctatcag gtctgcgaga tcctgacgac tgaagttgat ctcaaaacta
     6121 taagggcgtt cttcgtatcc ataagtacgc gcaaggagga ggagtacttc ggcaactttc
     6181 tcacgtacac tcttgtgtgc aaggatagca gctttctttt cagttgtttc gagctcttcg
     6241 agcaattcgt ttatgatctg ctctttgagt acgggacttt tttttactat ttcactaaac
     6301 agatgataag gaataaaaca atattggaca tcagtggtag ctgttgcaga attggtatgc
     6361 agaccgttct tgagattctc cctgtgaccg agtatagcgc ctttgctcac tacccggagt
     6421 ataatgggtt tgtcgattgt cgaat
//
LOCUS       DAOQQY010000157         6400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5175_length_6400_cov_5.092199, whole genome shotgun sequence.
ACCESSION   DAOQQY010000157 DAOQQY010000000
VERSION     DAOQQY010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6400
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5175_length_6400_cov_5.092199"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="PLE23_08245"
     CDS             <1..1024
                     /locus_tag="PLE23_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX83290.1"
                     /translation="NMTGIEAFISLTGLNFSTNNVSSIDLSKNTILESLDCSGNSLTF
                     LDITANTELWFLNCADNNISELDLVPQYRLQEFNCSGNSITALDLSKNEILFSLNCSA
                     NKLSYLNVANGNNPGISSIDAHNNAGLTCIQVDDAAFSKDKWVGTAYMFDGQQAFNEY
                     CEPPCKNAEDIKANFLFANSACVDENVRIIEYGIFTSIADSVIFKWDFGNGETSSERD
                     PIVTYKSAGDFKVRLTLENIECPLEITKEIMVFYCLKQANEEKISSVFPNPSLGDIML
                     NAKLDKPGDILVNIYTPDQKVVYSRMFTNVSELTEPILLNYKGMIVVEFIYAMGIEKH
                     KVILLH"
     gene            complement(1246..2487)
                     /locus_tag="PLE23_08250"
     CDS             complement(1246..2487)
                     /locus_tag="PLE23_08250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMX83291.1"
                     /translation="MKFDDYRISPDIKKSLEKLNFRKPTDIQFRVIPSVLKGEDVLAI
                     AQTGTGKTAAFAIPVIHKVQMGKIAGRANAIKCVVMVPTRELAVQIAEVFNKIGQYTR
                     VNTINIHGGVEQEPQIAKLTKGADIIVVTPGRLFDLVSQGFIKLDKVETVIFDEADHM
                     LDKGFLKDLQDLIRHLPVKRQTLFFSATINETIKKLAYSLVKNPIRIQLSPKDPVSKN
                     VDHAVAFITMDDKRFFLERLIHEHPDKKILVFVRTKVRAERVIKALARMSIPAVALHG
                     DKDQHDRLQTLGEFRGGQHKILVATDVSARGIDIPNVDYVVNYDIPDVPETYVHRVGR
                     TGRAFNKGYAVAFCSEEEKPLLQAIESYITKPVKVLDINRKDYTDTLAFTTDVNTDWK
                     SLLREEEQAEKAAKLKKRKKK"
     gene            complement(2535..3203)
                     /locus_tag="PLE23_08255"
     CDS             complement(2535..3203)
                     /locus_tag="PLE23_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX83292.1"
                     /translation="MSVVLESHHVHISQNKKQVLSDVSFSLNEGELVYLIGKTGSGKS
                     SLLKAIYGEIKPTSGSLIVAGTDMMKLKKSKLYLLKRKIGIIFQDHNLLMDRNVYDNL
                     YFVLKSTGWKNRAKMEARISEVLQKVGLSNKLADMPFELSGGEQQRLGIARALLNDPL
                     LIIADEPTGNLDPETSSNVFELLTNLAKEKGTAIIVATHDFKMIEDHPSKILVCKEGC
                     IHDA"
     gene            3398..3790
                     /locus_tag="PLE23_08260"
     CDS             3398..3790
                     /locus_tag="PLE23_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HMX83293.1"
                     /translation="MKKIHIVGLLMILGAIVVLMTAAKDLSTYDNFKSAGKATGAVKV
                     VGKLCKDKEIIYDPRKDPNYLSFYVQDNNNEIRKVVLKNAKPRDFEMSEQIVLTGTMK
                     GDEFLASDMLLKCPSKYKDEEIYVKSRS"
     gene            3809..>6400
                     /gene="ccsA"
                     /locus_tag="PLE23_08265"
     CDS             3809..>6400
                     /gene="ccsA"
                     /locus_tag="PLE23_08265"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMX83294.1"
                     /translation="MQDIQYIGESVLPGRIGHFLIITAFTSALLAAWSFFKANKLTGH
                     ESDSWKKLGRSAFTVHGLSIFSIIGLIFYLMANEHYEYQYVWAHVNSELPKRYIFAAF
                     WEGQEGSFLLWMFWHVVLGFIFLLWNNSWEKPVIGVLSLVQAFIVTMIMGIYFKLGEH
                     GLKIGSNPFMLLRETMDAPIFSNPDYLSKIQGKGLNPLLQNYWMTIHPPTLFLGFAST
                     VIPFCFAIAALINKKYTEWLKPVLPWALFSAFILGMGILMGAAWAYEALSFGGYWAWD
                     PVENMSLVPWLILIAALHSNVVSNATRYSTKPTFVLYIISFILVLYSTFLTRSGVLGD
                     TSVHAFTEMGLEWQLTIFMIFFTVWAGWLFFRNAKHIYVPKEEEKLNSREFWIFIGTL
                     VLVFSSVLITFTTSIPVFNKLFDAFGSLTGKDMSYLHRTAPTDAVAHYNKYQMWIAVL
                     IGILSGVAQYFRYKAFSWGTTIPKMLKSVLISMALAASMTWLTTYWINLVSWQYYILC
                     FVGYFGIFANLDYFIFYLKNNLKLSASVLSHLGFGVMIIGVLASGLNKRHISENRFAL
                     EGILDEDKIGKNIVLLKGVPMVMNDFKVTYLSDTMYNHTKEFNINYTRMDKQGNAVES
                     FDIQPYILYTNDFSKLASTNPSTKHYLTKDIFSIVSNLPKEEVEPEAAKQKEDSLRYK
                     LYQIGLNDTVVTDKNIITVAGINKNPELKHFDLKEGDIPLGVKLRVFNKKNDTAYIAT
                     ASIVLRDKFVYDFPAIFNEAGTKIKLKEDAAQAIFLENEESNVQSFSFKRGETQEYAG
                     MKITFRDFNKNVKHPLYKHQKDDIAVTAIMDIEAGGKKYTAEPLYLIRNGSPFVLKDM
                     ITDLGLNL"
BASE COUNT         1790 a         1238 c         1480 g         1892 t
ORIGIN      
        1 caacatgacc ggtattgagg catttatttc tctcaccgga ctcaatttta gtaccaacaa
       61 tgtttcgtcc atcgatttaa gcaaaaatac aatacttgag tctttggact gttcaggcaa
      121 ttcactgaca tttctggata taacggcaaa tacggagttg tggttcctca attgtgctga
      181 caacaatatt tcagaattag atttggtacc acagtatcgg cttcaggaat ttaattgctc
      241 gggcaacagc attactgcct tagacctgag caagaatgaa attctcttta gtttgaattg
      301 tagtgccaat aagctgagct acctaaatgt agccaatggc aacaaccctg gaatatcatc
      361 cattgacgct cataataatg cgggtctgac gtgcatccag gtcgatgatg ctgcctttag
      421 caaagataaa tgggtaggta cggcctatat gtttgatggt caacaggcgt tcaatgaata
      481 ttgtgagcct ccttgtaaaa acgctgaaga tatcaaggct aattttttgt ttgctaactc
      541 tgcgtgtgtt gatgaaaatg ttcgaatcat cgaatatggt atcttcacat cgattgcaga
      601 tagtgtgata ttcaaatggg attttgggaa tggtgaaaca tcttcagaaa gagacccgat
      661 tgtgacatac aaatcagcag gagattttaa agtacggctt actcttgaaa atattgaatg
      721 tcctttagaa attactaagg agattatggt tttttattgt ctaaaacagg caaatgaaga
      781 aaagatttca tcggtatttc ccaatccatc cttaggtgat ataatgttga atgcaaagtt
      841 ggataaacca ggtgatatat tggtaaacat ctatacccct gatcaaaaag tggtttacag
      901 taggatgttt acgaacgttt cagaattgac agagccaata ttgttgaact ataaaggtat
      961 gatagtagtt gagtttatct atgccatggg aattgaaaaa cataaagtga ttcttttaca
     1021 ttagggcgca accattgtaa atacagcata caacagaaat aaataggggc gaaagaaaat
     1081 cctctaaata agtattaagt acattctcgg ttatattccc atcatgcgtg aaggacagaa
     1141 gcgggcacga agtaaagcgg atggcctgac ccgacagggg cacgcccaaa acaaagaaca
     1201 tagctcaact tatcttgaaa atataagaat caggttgttg gattcctatt tttttttgcg
     1261 ctttttcaat tttgcagctt tctctgcctg ctcttcttct cggaggaggc tcttccagtc
     1321 ggtgttgacg tcggtggtga atgcgagtgt gtctgtatag tctttcctat tgatatcgag
     1381 gactttgacg ggtttagtaa tatacgattc gatggcctgg agcaatggtt tttcttcttc
     1441 gctgcagaag gcgacggcat agcctttgtt gaatgcgcga cctgtgcggc ctacccggtg
     1501 tacgtaggtc tcgggcacat cggggatgtc gtagttgact acataatcga cattggggat
     1561 gtcaatacca cgggcactca cgtctgtggc taccagtatt ttgtgttggc cgcctctgaa
     1621 ttcgccaagg gtctgaaggc ggtcgtgctg gtctttgtcg ccatgcagtg ctactgccgg
     1681 tatgctcata cgagcgaggg ctttgatgac ccgctctgca cgtactttgg tacggacaaa
     1741 tacgaggatt ttcttgtcgg ggtgttcgtg aatgagcctt tcgaggaaga atcgtttgtc
     1801 gtccatggtg atgaaggcaa ctgcgtgatc gacgtttttg gaaaccggat ctttgggcga
     1861 tagctggatg cgaatcgggt ttttgacgag tgaataggcg agttttttga tggtttcgtt
     1921 gatagtagcg gagaaaaaga gtgtttgtcg cttgaccgga aggtggcgga tgaggtcttg
     1981 gaggtctttg agaaaaccct tgtcgagcat gtggtcggcc tcgtcaaaga tgactgtttc
     2041 gaccttatcg agcttgataa aaccctggct gacgagatcg aacaggcggc ccggtgtgac
     2101 gacgatgata tcggcacctt ttgtaagctt ggctatctgt ggttcttgct ctacaccgcc
     2161 atgaatgttg atggtattga cgcgggtgta ttgtccgatt ttgttgaata cttcggcaat
     2221 ctgtacggcg agttcgcgtg taggcaccat cactacacat ttgatagcat tggcacgtcc
     2281 ggcgattttt cccatctgta ctttatgaat gaccggtatg gcaaatgctg ctgtcttgcc
     2341 ggtacctgtc tgtgcaatgg caagtacgtc ttctccttta agcacagaag ggatgaccct
     2401 gaattggata tcggtgggtt tgcgaaagtt gagtttttca aggcttttct tgatatcggg
     2461 acttatgcgg taatcatcaa atttcattca tcgtattttt atgatctgtt ctatcgtaaa
     2521 ttgcagataa tagattaagc atcatggata caaccttctt tacaaaccag gattttgctt
     2581 gggtggtctt caatcatttt gaaatcatgt gtggcgacaa tgatggctgt acctttttct
     2641 ttagcgaggt tggtaagcag ttcgaacacg ttggaggatg tctcggggtc gagattgccg
     2701 gtgggttcgt cagcaatgat gagcagcggg tcattaagca gggcgcgagc tataccgagc
     2761 cgttgttgtt cacctccgga gagctcgaaa ggcatatcgg cgagtttgtt gctgaggcct
     2821 actttctgta gcacctccga tatgcgggct tccattttgg cgcggttttt ccatccggtt
     2881 gatttgagga caaagtagag gttatcatat acattcctgt ccatcagcag gttatgatcc
     2941 tgaaatatga tacctatctt gcgtttgagt aagtagagtt tcgatttttt aagtttcatc
     3001 atatctgtac cggctactat gagcgaacct gaagtaggtt tgatttcgcc gtagatggcc
     3061 tttagcaggc ttgacttgcc cgagccggtt ttgcctatga ggtaaaccaa ttcgccttcg
     3121 ttgagcgaaa aactgacatc cgacagaacc tgttttttgt tctgactgat atgtacatga
     3181 tgagactcca atactacaga catgttaaac attataatta tatcacaaaa ttattaaatt
     3241 tactggcaat accagacttt tggctataat aaaagagcag ctgtctgcta caatgacaat
     3301 attgaaagag gtattactcg attatttaac agaaaatggc atagcatatc aacttattgt
     3361 gtttatattt gtagtctaat caggacaaac atttattatg aaaaagatac atatagttgg
     3421 cttgcttatg atattgggtg ccatcgttgt tctgatgacg gcggctaagg atctgagcac
     3481 ctatgataat ttcaaatcgg ccggtaaggc taccggtgct gtaaaggttg tcggcaagct
     3541 ttgcaaagac aaagagatca tctacgaccc tcgtaaagac ccaaactatc tgagttttta
     3601 tgtgcaagac aacaacaatg aaataagaaa ggtagtgtta aagaacgcca agccgcgcga
     3661 cttcgaaatg tctgaacaga tcgttctgac aggaaccatg aaaggtgatg agtttcttgc
     3721 ctcagatatg ttgctgaagt gcccttcgaa atacaaggac gaagaaattt acgtcaagtc
     3781 gagaagttaa cctcctacaa tacatttcat gcaagatata cagtacattg gggaaagcgt
     3841 cctgccaggt aggattggtc actttcttat cattaccgca tttacatcgg ccttgctggc
     3901 agcatggtct ttttttaaag ccaacaagct tacagggcat gaatcggaca gctggaagaa
     3961 gcttggtcgc tcagctttta cagtgcatgg cttatcgata ttcagtatca tcgggcttat
     4021 attctacttg atggccaatg agcattacga ataccaatac gtgtgggcgc atgtcaactc
     4081 cgaattgccc aagaggtata tatttgctgc attctgggaa ggacaagaag ggagtttttt
     4141 gctgtggatg ttttggcacg tggtacttgg ttttatattt ttattatgga ataacagttg
     4201 ggagaaacct gtaatcggtg ttttgagttt ggtgcaggca ttcattgtca cgatgataat
     4261 gggtatatat tttaagctgg gcgaacacgg tctgaagata ggttcgaatc cattcatgct
     4321 tctacgcgag acgatggatg caccaatatt ttcgaaccct gattatctga gcaagataca
     4381 gggcaaaggg ctcaacccgc tgttacaaaa ttattggatg accatccacc ctcctaccct
     4441 gtttttaggt tttgcctcaa cggtcattcc attttgcttt gccattgccg cattgatcaa
     4501 taagaaatac accgagtggt taaaaccggt attgccctgg gcattgtttt cggcattcat
     4561 attaggcatg ggcatattga tgggcgctgc atgggcttat gaagcattat cttttggtgg
     4621 atactgggca tgggaccccg tagagaacat gtcattggtg ccgtggctga tactgatagc
     4681 agccttgcac agtaatgttg tgagcaatgc aacccgatat tcgaccaaac ccaccttcgt
     4741 attgtatatc atcagtttta tactagtatt atattctacc ttccttaccc gatccggagt
     4801 gcttggcgat acctcggtac atgcatttac ggagatgggg ctcgaatggc agctaacgat
     4861 attcatgata ttttttacag tgtgggcagg ttggcttttc tttaggaatg caaagcatat
     4921 atacgtaccg aaagaggaag aaaagcttaa ttcgcgagag ttctggatat tcattggtac
     4981 gctggtgctt gtgttttcat ctgtacttat aacatttacc acatccatcc ctgtgttcaa
     5041 taaattgttt gatgcctttg ggtcgctcac cggcaaagac atgtcgtatc tgcaccggac
     5101 agctcctaca gatgctgtag cacattataa caaataccag atgtggatag cagtactgat
     5161 agggatattg tcgggtgttg cacagtattt taggtacaag gcattcagct ggggtacgac
     5221 aatacccaaa atgcttaaat cagtattaat atccatggca ttggcagcct ctatgacctg
     5281 gcttacgact tattggatca atttggtttc gtggcaatat tacattctgt gttttgttgg
     5341 atattttggc atattcgcca accttgacta cttcatattt tatttaaaaa ataatctgaa
     5401 actatctgct tcggtactta gtcacctggg cttcggggtg atgatcatcg gtgtattagc
     5461 gagcgggctc aacaaaaggc acatttcgga aaacagattt gcacttgaag gcattcttga
     5521 tgaagataaa ataggtaaaa acattgtact actcaaaggt gtgcccatgg tgatgaatga
     5581 ttttaaagtt acatacctga gtgacaccat gtacaaccac accaaggaat ttaacatcaa
     5641 ttatacccga atggacaaac agggaaatgc tgtggaaagc tttgatattc agccatacat
     5701 actttataca aatgactttt cgaaactagc ctctaccaat ccatcaacca aacattacct
     5761 gacaaaagat atcttttcca ttgtatcaaa tctgccaaaa gaagaagtag aacccgaagc
     5821 tgcaaaacaa aaggaagata gccttagata caaattgtac cagattgggc tcaatgatac
     5881 tgtcgtaaca gacaagaaca tcatcactgt agccggaatc aacaagaatc ctgagttgaa
     5941 gcattttgat ctcaaagaag gtgatatacc acttggcgta aaactgcgtg tattcaacaa
     6001 aaaaaatgat acagcctata ttgcaacggc atctattgta ttgcgtgaca aatttgtgta
     6061 cgacttcccg gcgatattca acgaagcagg caccaagata aaactcaaag aagatgctgc
     6121 acaggcaata ttcctcgaaa acgaagaaag caatgtacag tctttcagtt ttaaacgcgg
     6181 cgaaacacaa gaatatgccg gaatgaagat tacgttccgt gattttaata aaaatgtaaa
     6241 acatccgctt tataagcacc agaaagatga cattgcggtc actgccatta tggacatcga
     6301 agccggcggt aaaaaatata cagccgagcc gctttacctg atacgcaacg ggtcaccgtt
     6361 tgtactgaaa gatatgatca cagatcttgg gctgaatctg
//
LOCUS       DAOQQY010000158         6379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5202_length_6379_cov_6.868596, whole genome shotgun sequence.
ACCESSION   DAOQQY010000158 DAOQQY010000000
VERSION     DAOQQY010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6379
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5202_length_6379_cov_6.868596"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1010..2581)
                     /locus_tag="PLE23_08270"
     CDS             complement(1010..2581)
                     /locus_tag="PLE23_08270"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83295.1"
                     /translation="MKHILAYILLILLLSCLNTVMSGQTFSKTYNFNDGIDAGFKVFK
                     HNDNYFVQFWGENKDYSDPSVIHVTNYMGFLRTDLEGNLITSKIYDSLICYKTMWLKG
                     DKLLMAGNKSTKPDFEKKIYLLTTGYDGEIQDEKKLEFNTGNHRVSDSGRDQNKNYYL
                     TDRVIYYGDNNKEKLYFNDTILLHKWDSTLNYKKTIMVPFLQPITYGVNTAVHNDNYF
                     VSSIMYFDTIKGSGSSTRTAVVAYDSTGQLLWQYNPLKNRITGGTNVVTETAGFKDSS
                     TAVLMSYDYLGNTWQQVIKFNNKGEKLWEYDDKPEHPFYYYTYFSIFKTKDDGVILCG
                     ENSFFDFDPDWDKASAIIAKLDKNGNLQWRRYIWDHNVQARRCGFINGMELDNGNFII
                     TGTLVDSTGFLQFDTRVWLMLLDSVGCYNPGCGKFDSITATQNIVLVGKDMLKLSPNP
                     AHDYVRVSWNNTPTPAERLLVYDLKGRTVAEKTVHDRSGSIELSVTDLPAGTYLVKIQ
                     GNKWESPPEKMVRQK"
     gene            complement(2644..5679)
                     /locus_tag="PLE23_08275"
     CDS             complement(2644..5679)
                     /locus_tag="PLE23_08275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83296.1"
                     /translation="MLSTSLQLSAQSSDPPLGYECNGILGDSPEMPLVLTNCPFSSIL
                     PEDLSALPVATIHVNFHFIRFKGDLKLGPGNFKPDLSWGEKLCGDKIAQLLVDGCNND
                     AMNVAANPLNLAVGYPNYIGDSKLRLRIVGNGNGNGIYYWDEKPSSFPNNNVVNIIID
                     SDTKYNVNGYTNFNSKNIYLNNMYKLIFTDSANWWDYKNLLWHELGHAFGILCHSFET
                     QGNCANEDINTPAECGTGPGNNDCGSPDKPGCSNWSTQSNNIMGYNGKVPWALSPCQW
                     TAFYSGIYTTSHKSTIDFCSDNSQPPIIIPSGAFITWSTLKLLNRDVIVQTGAQLTIN
                     CEVRMGADRTIKVERGAKLIVDDGAIRNLCFEKRWGGIYVEGNNTLPQPDPNSFPGLN
                     EAGIVFVKNGSVIEGAADAMITARPGELWNEQYWGGVMYAENSSFVNNRRVAAFMRYT
                     FPNKSKFRHCSMNESGSLFNSYGVTVWSCKGITFEGNDLGPFDNQAIYGIDMGIEVLD
                     DNTIHNCQNGIESLVTTPLVGNIINVDGSSTPNKFMNNKKYDILLHGSDLSLINSINS
                     NYFLPGSSSSVTGVAVDGYATVDIKNNSFANRDFSILLSDAGNLSKLHCNIFESNTSN
                     GIVLTGNNEKTLFYNNTFYAGHLNISVLPQASQPGIVFSPQNSSGSSNPSNSLAADNC
                     FYVGNKQDVYANPSNTVLFNYKRRVPDGSCYIPNNHLSDGGVNNYMVADAGPSVPDCD
                     DNSFDLPPTTEEYVTWRTYTQYLKSMAEAMPANSTLVDSYHLAELKLSHWRTLLVSQA
                     LTTHEYTTIEGILAPETNIIAQKMKFGIALASNNYTKAQSILDQLPDASLEDQYFKYT
                     QQVCLQINQLPWSDTLTIAQLTQLHLIAQSELPSKVYARGILALKTGATFEPLTVLMP
                     VLPETREAAQSESSVDDNSWSVSPNPAENVLNIQMEENKLGALLHVVDVFGKTWINNE
                     LHEGTTTAQIDLSKIPVGLYIAVLQKRGQTERMRKFVKK"
BASE COUNT         1823 a         1326 c         1201 g         2029 t
ORIGIN      
        1 ctttcttgaa aaaaataatg tgagtgcttg gtttttacca tagaatacgc ggcatgggtc
       61 agtggttagt tgttagttgt aagttgtaag ttgttagttg taagttgtaa gtgaaatgta
      121 agatgtatgc tgttgtgaaa cattaatatt cagcattttt tgaaccattc agaagttaag
      181 agaattaagg cggatactgg gccgggcaag cggaaagttg gacaaaaaaa atctccgttt
      241 tgtttgcctc tttcctaaag ggagcaaaac gaagattttg atggaaaatt gaactgtttt
      301 tctttccgcc tcccttcagg gccggggcaa gcggaaagag agcaactctt ttcgtctgaa
      361 tctcggatgg cacctattgg ctatatgcca gaaaatttct cttacaagga gactgtcagc
      421 accacatttt acctcatccg gccgttggcc accttctcct gattccaatc aggagaaggc
      481 actaaaaatg caaatagaaa aaaaaataga gtctccttct ccgtttcgcc ggaaatggag
      541 agggccgggg tgaggcaggg agaagtgcaa gatatctgac atatacaaaa gctaaaatcc
      601 catagggatg acatgtttat agaaaaaaaa tccgaagatg gaaaaaccct gaaagggtga
      661 catgttgctg aaccgcaaaa tcctcgtttt gtctgcccct taccctaaag ggagcaaaac
      721 gaagattttg atagaaattc gtaccactta catctcactt ttcacttttc acttctcact
      781 tttcactcaa aaagaccggc atgaggtcag catagcctga gcgggcggac agcatctcgt
      841 ttccttgtca gcatcaggat tttcaggatt ccaggaattt tcaggatttt atttcggacg
      901 atgatggctt agggcaagca gaaagttgta taaaaaccaa caccattcac tactcaacac
      961 ttaccactta catctcactt ttcacttttc acttttcact tctcacttct cacttctgcc
     1021 ttaccatctt ctctggtggg ctctcccatt tgttaccctg tatttttacc agataggtgc
     1081 ctgcaggtaa atcagtgact gacaactcta tacttcccga cctgtcgtgt actgtttttt
     1141 ctgccaccgt tcggcctttc agatcataga ccagcagcct ttctgcaggt gtgggtgtat
     1201 tattccagct tacccttaca taatcgtgcg cagggttagg gctgagcttg agcatatctt
     1261 tcccgacgag tacgatgttc tgcgtagccg ttatggaatc aaacttgccg cagccggggt
     1321 tataacaacc taccgagtcg agcaacatca gccaaacccg agtgtcaaat tgcaaaaaac
     1381 ctgttgaatc gaccagtgtc ccggtaatga taaaattccc gttgtcaagc tccatcccat
     1441 taataaaacc gcaccttctg gcctgaacat tatgatccca aatataccgc ctccactgta
     1501 gattgccgtt tttatcaagc ttggcgatta tcgcactggc tttgtcccaa tccggatcaa
     1561 agtcaaaaaa tgaattctct ccacataata taacaccatc atctttggtt ttgaatatcg
     1621 aaaagtaggt atagtaataa aaggggtgct ctggtttgtc atcgtactcc cacagctttt
     1681 cgcccttgtt gttgaatttt atgacctgct gccaggtatt accaaggtaa tcgtaagaca
     1741 tcagcactgc tgtacttgaa tccttaaatc ctgctgtttc agtaacaaca tttgtaccac
     1801 ctgtaatacg gttcttgagg ggattgtact gccacaggag ctgccctgtc gaatcgtaag
     1861 ccaccacagc cgttcgagtg gaagaccccg atccttttat ggtatcaaaa tacatgatag
     1921 agcttacaaa gtaattgtcg ttgtggacgg cggtatttac accataggtg ataggctgaa
     1981 gaaatggcac cattatggtt tttttgtaat tcaaagtact atcccacttg tgcaacagga
     2041 tggtatcgtt aaaatacagt ttttctttgt tattgtcccc ataataaatc actctatcag
     2101 tcagataata attcttgttt tggtctctac cagaatcact tactctgtgg ttgccggtat
     2161 taaactcaag ctttttttca tcctgtatct ctccgtcgta gcctgttgtc agtaaatata
     2221 tctttttttc gaagtcaggc ttagtacttt tgttaccggc catcagcaat ttgtctcctt
     2281 tgagccacat ggtcttgtag catatcagac tatcataaat tttactcgta atcaagttgc
     2341 cttcaaggtc tgttcttaaa aagcccatgt agtttgtaac atgaataact gaggggtcac
     2401 tgtagtcctt attttcaccc caaaactgta caaaataatt atcgttgtgc ttgaaaactt
     2461 taaaaccggc gtctatgccg tcgttaaaat tataggtttt actgaaagtc tgcccactca
     2521 ttactgtatt caggcatgac agcagcaaaa tcaatagtat ataggctaga atatgtttca
     2581 tatcttatgt attattaagt aaggagcagg ctatgtctgc tccttactgt atgttaaaaa
     2641 ttattatttc ttgacaaact tgcgcattct ctctgtttga ccccgcttct gcaatacggc
     2701 tatatacaga ccaaccggta tttttgataa atctatctga gctgtagttg ttccttcatg
     2761 cagttcatta tttatccatg ttttgccgaa tacatccact acatgtagca aggcgcccag
     2821 tttattttcc tccatttgta tattgagtac attctcagcc gggttaggtg atacagacca
     2881 actattatca tccaccgatg attccgattg ggctgcttct cgcgtctcgg gtagtacggg
     2941 cataagcact gtaaggggct caaacgtggc tccggttttt aacgcaagta ttccccttgc
     3001 atatactttt gatggcagct cactttgtgc tatcagatgc aattgtgtaa gctgtgcgat
     3061 ggttaaggta tcggaccatg gcagctgatt tatttgaagg catacttgtt gggtatattt
     3121 aaagtattgg tcttctaatg aagcatcagg caattggtcc aatatgcttt gtgcttttgt
     3181 gtagttgtta ctggcaagcg caatcccaaa tttcattttc tgggcgataa tgtttgtctc
     3241 tggagccagt attccttcta tggtggtata ttcatgagtg gtcagagctt gggacaccaa
     3301 cagtgtgcgc cagtggctca gttttagctc tgccagatga tacgaatcta caagagtact
     3361 gttggcaggc atagcctcag ccatgctctt caaatactgt gtataagtac gccatgtaac
     3421 atattcttca gtcgttggtg gcaggtcaaa agaattatcg tcacaatccg gtacagacgg
     3481 tccggcatct gcaaccatat aattgttgac tccaccatcc gatagatgat tgtttggtat
     3541 gtaacaggag ccgtcaggta ctcttcgttt gtaattaaac aaaacggtgt tgctagggtt
     3601 tgcatataca tcttgtttat tacccacata aaagcagttg tcggctgcta atgaattgga
     3661 tgggttactg gaccccgatg aattttgtgg gctgaacact atcccgggtt gagaagcctg
     3721 tggcaatacg gaaatattga ggtgacctgc atagaaagtg ttgttgtaaa ataaggtctt
     3781 ttcattatta ccggtcaaca ctatcccgtt tgatgtattt gattcgaata tgttacagtg
     3841 caatttactt aggttccctg catcacttaa aagaatacta aaatctctat tcgcgaatga
     3901 attattttta atatccaccg ttgcataacc gtcaacagct acccctgtta ctgatgacga
     3961 actacccggt aagaaatagt ttgagttgat actgttaatt aagctcagat ctgaaccatg
     4021 tagcagtata tcgtattttt tattattcat aaatttgttt ggtgttgagc taccatctac
     4081 gtttatgatg ttgccaacca agggagtagt cacaagactt tcaataccat tttgacaatt
     4141 atgtattgtg ttatcatcca acacttcaat acccatgtca ataccataaa tggcctgatt
     4201 atcaaaaggt ccaagatcat taccttcaaa agtaattcct ttacaactcc aaacggtaac
     4261 gccataggag ttgaacaatg acccactttc attcatggag caatgcctga atttgctttt
     4321 atttggaaat gtatatcgca tgaacgccgc cacccgtcgg ttgttgacaa aacttgagtt
     4381 ctcagcatac ataactccac cccaatattg ttcattccac aactcgcctg gtctggcggt
     4441 aatcatggca tctgctgctc cttctataac agatccattt tttacaaaaa ctattccggc
     4501 ttcgttcaga cccggaaaac tgtttggatc tggttgagga agtgtattat tgccttcaac
     4561 atagatacct ccccatctct tctcaaaaca aagatttcgt atggcaccgt catccactat
     4621 caattttgct ccacgttcca cttttattgt cctgtctgca cccatccgta cctcacaatt
     4681 aatagttaat tgggcacctg tttgaactat aacatcccga ttgagcaact taagagtact
     4741 ccaggttatg aaagcccctg atggtataat gatgggaggc tgggagttat cagagcaaaa
     4801 atcaatagtt gatttatggg aagtagtata tatgcctgaa taaaatgcag tccattggca
     4861 aggtgataga gcccatggca ccttaccatt gtaacccatt atattgttgc tttgagtact
     4921 ccagttgcta caaccaggtt tatccggtga gccacagtca ttattacccg gccctgtacc
     4981 acattcggca ggtgtattaa tatcctcatt ggcacaattg ccttgggttt caaatgaatg
     5041 gcataaaatg ccaaaagcat ggcccaattc atgccaaagt aaatttttat aatcccacca
     5101 attagcactg tctgtaaata tcaatttata catattgttt aaatatatat tttttgaatt
     5161 gaaattagta taaccattta cattatattt tgtatcagaa tcaataatta tatttacaac
     5221 attattatta ggaaatgatg aaggtttctc atcccaataa tatattccat tgccatttcc
     5281 attgcccact attctaagtc ttaactttga atcaccaata taattggggt agccaactgc
     5341 cagatttaga ggattggcag caacattcat agcatcatta ttgcacccat ctaccaaaag
     5401 ttgtgctatt ttatctccac ataatttctc accccaagat aagtcaggtt taaaattgcc
     5461 gggaccaagt ttcaaatcac ctttaaatct aataaaatga aaattaacat ggatggtagc
     5521 aactggcaaa gcagataaat cttcaggcaa gatactacta aaggggcaat tggtaagtac
     5581 caaaggcatt tctggagaat cccccaaaat gccattacat tcataaccta agggtgggtc
     5641 agacgattga gctgataact gaagggaagt cgaaagcata agtacaacaa ttaaaattgc
     5701 caaattataa attacccccc catgtatgac agaatataat ttggtaataa ttttattttt
     5761 ttccgataat tttgttctct tttcatgatt tatgtagttt ttattaaaaa atgaataaaa
     5821 aatcaaatat aaaataaaag taatgctatg tcaagtaaag ctaattattt ttttaaaaat
     5881 ttatttcgat attcattttt actgttagat tttattatat tcgtctggtg gcatcttttg
     5941 ctttcacaat ctatatcaca cggatgatct tgtaataatt caggcatttt tagaaccatt
     6001 cagaagttaa gcgtatgaag cagatactgc gccgggcaag cggaaagtta tacaaaaatc
     6061 tccgttttgt ttgcctcttt cctaaaggaa gcaaaacgaa gatcttgcgg ttcaacatcg
     6121 tgtcaccctt ccagggttat ctacattcgc atgcttgttt ctataaacat gtcatcccta
     6181 tgggattttg acatttgctt atgtcagata ttttgaactt cctctaccgc accccagcct
     6241 ctaatgggag caaaacgaag atctttgaag gaaattcgta ccatttacct ctcacttttc
     6301 acttttaatt ttttaattgt taattcaatg tcgtttagtt aagcgaaaat acggataaaa
     6361 atcttaaatt tgtgatgca
//
LOCUS       DAOQQY010000159         6375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5215_length_6375_cov_5.902690, whole genome shotgun sequence.
ACCESSION   DAOQQY010000159 DAOQQY010000000
VERSION     DAOQQY010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5215_length_6375_cov_5.902690"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLE23_08280"
     CDS             complement(<1..776)
                     /locus_tag="PLE23_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83297.1"
                     /translation="MIEIIAWVGVSQALFASFLIFTKKENSQSDKVLSLWLALLALDF
                     LSCALDYLIFSKPLLSSSFLLFNPALFLYICSLTDRKFRLRPVYLLHLVPYLLFKLIA
                     YWVQEAFAMNTFFDRDSFFLYRIAFGTATVISSIVYNFLSLRLVHRHRVSLQAERSNI
                     DQNESIGWVLFVSVSYTVYCIIAVVVAMVSYFTKSYPLSPHIYNYAVLLGLIYILSFY
                     GLYQLRLSLFSEDIEEERIPYQKSLLTTEAKGQIQEKIEE"
     gene            complement(1012..2463)
                     /gene="asnS"
                     /locus_tag="PLE23_08285"
     CDS             complement(1012..2463)
                     /gene="asnS"
                     /locus_tag="PLE23_08285"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HMX83298.1"
                     /translation="MSKRIKIAELLQSSKTGTTVNVKGWIRTFRNNQFIALNDGSTNT
                     NIQVVVDFENTDPAELKRYTTGAAIGVEGELVSSQGKGQTVEIIARRIEVYGDCDAEV
                     YPLQPKKHSLEFLREIAHLRFRTNTFAAVFRVRHALAFAIHKFFNDKGFVYIHTPIIT
                     ASDAEGAGEMFRVTTLDAVKPPMNEAGEVDFSKDFFGRAANLTVSGQLEAELGALALS
                     EVYTFGPTFRAENSNTTRHLAEFWMIEPEVAFADLQDNMDLAEDMLKYVIRYAMDNCR
                     EDLEFLSNRQAEEEKSMPQNERSEMSLTQKLEFCLANDFERITYTEAIDILRNSNPNK
                     RKQFKYLIDGWGADLQSEHERFLVEKHFKKPVILTNYPAEIKAFYMRQDDPKEGVPGP
                     TVAAMDILFPGIGEIVGGSQREERLDRLTAKMREMHIPEEEMYWYLDTRRFGTCPHAG
                     YGLGFERLVLFVTGMTNIRDVIAFPRTPGNAEF"
     gene            complement(2738..3325)
                     /gene="lptC"
                     /locus_tag="PLE23_08290"
     CDS             complement(2738..3325)
                     /gene="lptC"
                     /locus_tag="PLE23_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMX83299.1"
                     /translation="MLKLQILPKNILTLLLTCVIFTVLFITQGCKDDIISHDLRITPG
                     DTQKEVLNDVEILYSDSAMVRVRLTGPELIRKYEQERTKEEFTKGVFVEFYDETGKVT
                     STLKADYAFRSEGADEITVQGNVLLTSSKKETMETDELIWNQKEEEVYTEKFVLVKTA
                     KERAWGHGFVSNQNFTEWRIKGFEGKLPSEKFGVE"
     gene            3532..4041
                     /locus_tag="PLE23_08295"
     CDS             3532..4041
                     /locus_tag="PLE23_08295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6265 family protein"
                     /protein_id="HMX83300.1"
                     /translation="MMIKRHLPLSIFLIAGILLTSCVASKKFTEISKLGIKKNFWLLG
                     SWNQNKSNTVEEWKFINKNLITGRMYSAVKGDTIIAETYKLERKGKEVFFTMKSTEST
                     IPQVEMKLKSETPGVLIFENPQQEFPRTISIKLDGTSKTTLFEGINKDGEGVTRDFYF
                     TKSEPAVKK"
     gene            4075..4542
                     /gene="smpB"
                     /locus_tag="PLE23_08300"
     CDS             4075..4542
                     /gene="smpB"
                     /locus_tag="PLE23_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HMX83301.1"
                     /translation="MAEIKKPRLTEIRNRRVEFEFKLLNTFEAGLILTGTEVKSLREG
                     HANLNDAYCYIRQGELFVRNMFISEYDLGTYNNHDPRRPRKLLLTRNELNKLGKKVKE
                     KGFTIVPVRLYFSERGLAKLEIALAQGKKSFDKRHSIKEKDTKRDLDRLKRYK"
     gene            complement(4577..5794)
                     /locus_tag="PLE23_08305"
     CDS             complement(4577..5794)
                     /locus_tag="PLE23_08305"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMX83302.1"
                     /translation="MTTEIIITVCSLLILAYLFDVSSAKTKIPPVILLLLVGWAFSQG
                     IHFFGIEIPDMTPILPIFGTIGLILIVLEGSLELEFHVSKIGFIAKSLIIAFVPILVF
                     SFGLAYTFEYFGNVPLKIGLANAIPLAVISSSIAIPSAQNLIHSQREFITYESSLSDI
                     FGVIFFNFVTLNDSIGAHTFNNFVVEMLIILVVSFVATLVLAFLLSRIKHPVKFMPII
                     LMIIMIYTVAKIFHLPGLVFILLFGLFLGNLDELKSFKFIEKFRPDILNLEVVKFKEL
                     TTEVVFLVRAIFFLLFGYLIETEELLNPNTVVWALSITAGIFVLRFVLLKVFRMKLNP
                     LLYIAPRGLITILLFLSIPENQVIDLVDKSLIIQVIIFSALIMMFGLMINKKEPEQSE
                     VLPDNNIQEVADH"
     gene            complement(5976..>6375)
                     /locus_tag="PLE23_08310"
     CDS             complement(5976..>6375)
                     /locus_tag="PLE23_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX83303.1"
                     /translation="PEIKELIDYEQFCGVRAVEEKMKEISVAEFEKWLDNNEDIQIID
                     VREPEEYQQVNIGALLVPLATIKERAAEIRKDKKVVVHCKAGGRSAKAIRELQDAFGF
                     DNLYNLKGGIIAIEHETSILSKKRSTAEAV"
BASE COUNT         1960 a         1357 c         1215 g         1843 t
ORIGIN      
        1 tattcttcta tcttttcctg tatctgtccc ttggcttccg tagttagcaa agatttttgg
       61 tacggaatcc gttcttcttc gatatcctcg gaaaaaagac tcaggcgcaa ctgataaagc
      121 ccataaaaac tgagtatgta tatcagtcca agtaaaacag cataattgta aatatgtggt
      181 gagaggggat agcttttggt gaaataactt accatagcga ccactactgc tatgatacaa
      241 taaacagtat atgacaccga aacgaacaag acccaaccga tgctttcatt ctgatcaatg
      301 ttggagcgct cggcctgcag gctgaccctg tgtctgtgca ccaacctaag gctcagaaaa
      361 ttgtaaacga tagacgaaat aaccgttgct gtcccgaaag caattctata aaggaaaaaa
      421 ctgtcgcggt caaaaaaggt attcatagca aaagcttcct gaacccaata ggcaatcaat
      481 ttgaacagca agtatggtac cagatgcagt aaatataccg gcctgagcct aaatttcctg
      541 tccgtcaacg agcagatgta caagaaaagt gcaggattga ataaaagaaa ggaacttgac
      601 agcagtggtt tgctgaagat caggtaatcg agcgcacagc tcagaaaatc cagtgccagc
      661 aacgccagcc acagactaag caccttatct gactgactat tttctttctt ggtgaaaatc
      721 aagaaagacg caaacagtgc ctgactgaca cctacccatg caattatctc aatcacgtat
      781 gtaatctgtt ttgatcgata ttaccattgc ataaatgtag gaataaaact gttaaatttg
      841 tcagcttgca gagcctaata tttcgttcgg cccaaaaata tccactctgt ctttagaatt
      901 gaatttttaa tttgcccaaa agacacatta ggaattgaac agctattaga ctacatagat
      961 gattttaaat actgattatc aaagcagaat atagtagcac taaagtccaa tttaaaactc
     1021 tgcattgccc ggagtacgtg ggaaggctat aacatcacgg atattagtca tgccggtaac
     1081 aaaaagcacg agacgttcaa agccaagtcc ataacctgca tgtgggcagg taccaaacct
     1141 tcgcgtgtcg aggtaccagt acatttcttc ttccggtata tgcatttctc tcatcttggc
     1201 agttagccga tcaaggcgtt cttctctttg tgagccgcct actatctcgc ctatgcccgg
     1261 aaacagaatg tccatagcag ctactgtcgg accgggaaca ccttctttcg gatcatcctg
     1321 acgcatgtag aaagctttga tttcggcagg gtaatttgta agaatgacag gttttttgaa
     1381 atgcttttct accaaaaacc gttcgtgttc actttgaagg tctgcgcccc aaccatctat
     1441 caaatacttg aactgctttc gtttgttagg gttcgagttg cgtagaatgt cgatagcttc
     1501 cgtgtaggtg atgcgttcaa aatcattcgc cagacagaat tctagctttt gtgtcaagga
     1561 catctctgaa cgctcatttt gtggcattga tttttcttct tctgcctgtc tgtttgacag
     1621 aaattcgaga tcttcacgac agttatccat agcatagcgg atgacgtact tcagcatatc
     1681 ttctgcaaga tccatgttgt cttgaaggtc ggcaaatgct acttcaggtt ctatcatcca
     1741 aaactcagca aggtggcgtg tagtgtttga attctcggca cggaatgtcg ggccaaaagt
     1801 atatacttcg ctaagagcta aggctcccaa ttctgcttct aactgacccg aaactgtcag
     1861 atttgcagca cgaccaaaga aatcctttga aaaatcaact tctccggctt cgttcatcgg
     1921 tggctttaca gcatccaatg tcgtaacacg gaacatttca cctgcacctt ctgcatctga
     1981 tgcggtaata ataggtgtat gtatataaac aaatcctttg tcattaaaaa atttatgtat
     2041 ggcaaaagct aaggcatgac gcacacgaaa aacagccgca aaagtatttg tccggaaacg
     2101 tagatgagct atctctcgca aaaactcaag cgaatgcttc ttgggttgta aaggataaac
     2161 ttccgcatca cagtcgccat atacttcgat tcgcctggca atgatttcaa cagtctgacc
     2221 tttgccctgc gatgacacca actcgccttc cacaccgatt gctgccccgg ttgtatatct
     2281 ttttagttca gcagggtcag tgttttcaaa gtcaaccact acttgtatat tggtattggt
     2341 actaccatca tttaaggcta tgaactgatt gttcctgaat gtgcgtatcc agccttttac
     2401 gttaactgtt gtccctgttt tgctcgattg taggagttcg gctattttga tgcgttttga
     2461 catgttattt attgaaaatt tgagtaagct aaatacgctt tattgaggtg caaagttgca
     2521 aaaaaatatt ccgcaaacaa gcagaaaaat tgtatttttt gaatttaacc cggattacgc
     2581 attaggctct tactgcaatt atttattagt tcaaatccat ttcaaaagct caaaaaaaca
     2641 aggttcacta tggttttatc acttttttat ggatttttgt acgaatcatt ttcgttcaaa
     2701 ttcctatgca ttatctgggt ttaaagcact gctgatatta ttctacacca aatttctcag
     2761 acggcaattt gccctcgaat ccttttatcc gccactcagt aaaattctgg tttgacacaa
     2821 agccatggcc ccaggcacgt tctttggctg tcttgacgag gacgaatttt tctgtataaa
     2881 cttcctcctc tttttggttc caaatcagct cgtccgtctc cattgtttcc ttttttgaac
     2941 ttgtaagtaa gacgttgccc tgtacagtaa tttcatctgc accttcgctt ctgaaagcat
     3001 aatctgcctt taaggtagat gtaaccttgc ctgtttcgtc ataaaattct acaaaaactc
     3061 ctttagtaaa ttcttcttta gtacgttctt gctcgtattt ccgaatcaac tccgggccag
     3121 taagccgcac ccgtaccata gcactgtcag aatataggat ctctacatca ttcaaaacct
     3181 ctttttgggt gtctccgggt gttatgcgaa gatcatgact gatgatgtcg tccttgcatc
     3241 cttgtgtaat gaacaatacg gtaaaaatta cacaagtcaa caatagtgtc agaatatttt
     3301 taggcaaaat ttgcagcttc agcacttaat catattattt cgatagtcaa aaatataaaa
     3361 acatctgtgt ataacgtagt tcgcttgcaa aatatacatt gtctgatgaa attcttcttt
     3421 gtataaacca aattattgga atatttcgcc tgcgataaag tccaaaacaa acatatataa
     3481 atattaataa acttttgtaa caggtatgcg tataagctaa ataaaatagt gatgatgata
     3541 aaaagacatc tgcctttatc aatatttctt atagccggca tattacttac ttcttgtgta
     3601 gcttcgaaaa aatttacaga aatttcaaaa ttgggaatca agaaaaattt ctggttgctc
     3661 ggttcgtgga atcagaataa atcgaataca gttgaagaat ggaagtttat caacaaaaac
     3721 ctgataaccg gaagaatgta cagtgctgtc aaaggagata ccatcattgc cgaaacttac
     3781 aagctcgagc gcaaaggcaa ggaagtattt tttaccatga aatctacgga aagtaccatt
     3841 ccacaggtag aaatgaaact caaatctgaa acgcccggag tactgatatt cgaaaatcca
     3901 cagcaagagt tccctcgtac tatttctatc aaacttgacg gtacgtcaaa aacaaccttg
     3961 tttgaaggta ttaacaagga cggcgaagga gtaacgcgtg atttttattt cacaaaatct
     4021 gaacctgccg ttaaaaaata gtaactttgc aggcctttat cactatgtat ttcaatggcc
     4081 gaaatcaaaa aaccgcgact tactgaaatt cgcaacagac gtgtcgagtt cgaatttaag
     4141 cttctcaata catttgaggc agggcttata ttgaccggta ccgaggtaaa atcattgcgc
     4201 gaaggccatg ccaacctgaa tgacgcttat tgttatatca gacagggtga actcttcgtt
     4261 cgcaacatgt ttatttcgga atacgatctt ggcacctaca acaaccacga tccacgcagg
     4321 ccgagaaaat tgctcctcac aagaaatgag ctgaataagc ttggaaaaaa ggtcaaagaa
     4381 aagggtttca ccatcgtacc tgtcagactt tatttttctg aacgtggtct ggccaaactc
     4441 gaaatcgccc ttgcccaagg caagaaatct tttgataagc gacattccat caaggaaaaa
     4501 gataccaaac gcgatctgga caggctgaaa agatacaaat aaaccatctt cctaatctcg
     4561 acaaatgaat acaacattag tggtctgcga cttcttgtat gttgttgtcc ggtagcactt
     4621 cgctttgttc cggttctttt ttgttaatca tcaggccaaa catcataatc aatgcagaga
     4681 agattataac ctggataatc agcgatttat ctaccagatc tatgacctga ttttcgggaa
     4741 ttgacaggaa caacagaatg gttatgaggc ctcttggtgc aatatagagc aatggattca
     4801 atttcatcct gaaaacttta agcaaaacaa acctcagcac aaaaatgccg gcagtaatac
     4861 ttaatgccca aacgacggta ttcgggttga gcagttcctc tgtttcaatt aaataaccaa
     4921 acagcaagaa aaatatcgcc cgaaccaaga agacaacctc cgtagtgagt tctttgaatt
     4981 ttacgacttc aagattcaat atgtccggcc gaaatttttc aataaactta aaggacttga
     5041 gttcgtccag attgccaaga aaaagaccaa acaacaggat aaacaccaga ccaggcagat
     5101 ggaaaatttt tgctactgta taaatcatga taatcattaa aatgatcggc atgaacttga
     5161 ccggatgttt tattctgcta aggagaaaag caaggaccaa tgtagcaacg aagctaacca
     5221 ctaatataat gagcatctcc actacaaaat tgttgaatgt atgggcgcca atagaatcat
     5281 tcagcgttac aaaattgaaa aagattacac caaaaatatc cgacaaacta ctctcataag
     5341 taatgaactc ccgttgtgaa tgaatgaggt tctgtgcact ggggatggca atcgaactgc
     5401 ttatgactgc cagcggtatt gcgttggcaa gtccgatttt taacggaaca ttcccaaaat
     5461 actcaaatgt gtaggcaagg ccaaagctga acacaagaat gggtacaaaa gcaataataa
     5521 gtgatttggc aataaagcct atttttgaaa catggaattc gagctcaagc gagccttcca
     5581 acacgataag tatcagaccg atcgtcccaa aaataggtag tatcggcgtc atatccggaa
     5641 tttctatccc gaagaaatga ataccttgac tgaatgccca ccctaccaag agtaacagga
     5701 tgacaggcgg tattttcgtc ttggccgagg aaacgtcaaa taggtatgcc agaatgagca
     5761 aggagcatac tgtaatgatt atttcggtcg tcatagtttg ttttttcggt caccgtttca
     5821 ttcatccgac aacatttcac aaggcatttc tgctccggca gccacgtcgg gcaatgaaaa
     5881 caaaatctgt ttgaaattct ttaaaaagaa tccaaatata acaatgtggt tgataaattt
     5941 taccaaaaat caaaaatatc tctgctccaa agcacctaaa ctgcttcggc ggtgcttctt
     6001 ttcttgctca aaatacttgt ttcgtgctca atggcgatga ttccgccttt gaggttgtaa
     6061 agattgtcaa atccgaaagc atcctggagt tcacggatgg ccttggcgct cctgccaccg
     6121 gctttgcaat gcacaactac ttttttgtct ttcctgattt cggcggccct ttctttaatt
     6181 gtggcaagcg gaacaagcag cgcaccaatg ttcacttgct ggtattcttc cggttctctg
     6241 acgtcgatta tctggatgtc ttcgttgtta tccagccatt tttcaaactc ggctacgctg
     6301 atttctttca ttttttcctc aacggcacgc acaccacaaa actgctcgta atcaataagt
     6361 tccttgattt caggt
//
LOCUS       DAOQQY010000160         6369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5222_length_6369_cov_5.740893, whole genome shotgun sequence.
ACCESSION   DAOQQY010000160 DAOQQY010000000
VERSION     DAOQQY010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5222_length_6369_cov_5.740893"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..588)
                     /locus_tag="PLE23_08315"
     CDS             complement(295..588)
                     /locus_tag="PLE23_08315"
                     /inference="COORDINATES: protein motif:HMM:NF020496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HMX83304.1"
                     /translation="MNLRYNFEAELWRHKGSAGWYFVALPDELSAEIRLFNQHLEEGW
                     GRLKARAQLGSSEWDTAIWYDTRQQCYLLPIKASIRLKEKVQCGDNVSVNIRI"
     gene            complement(588..1163)
                     /locus_tag="PLE23_08320"
     CDS             complement(588..1163)
                     /locus_tag="PLE23_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMX83305.1"
                     /translation="MLLEDLSSIEIFSVDEWRSWLEANHRSEHSVWVVCHTKKSVKPS
                     VGWSQMVDEALCFGWIDSTRKTIDEHRYRQLFSQRKPDSTWSMINKEKIEKLIAEGRM
                     TIAGMEAVERARNNGSWTILDEVEQLMIPEDLMQAFTLSPAAMQFYDTLSKSLKKVVL
                     QRIAMAKRPETRQKRIIEATQSLALNKKPVV"
     gene            complement(1291..3213)
                     /locus_tag="PLE23_08325"
     CDS             complement(1291..3213)
                     /locus_tag="PLE23_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83306.1"
                     /translation="MLKRPGVMQEDLMTLTRLVSAFAGVSSNFGNNAIVVRGNSPQSL
                     QWKLEGVEISNPNHFADLAAFGGGGITALSAQLLANSDFFSGAMPAEYSNALSGVFDI
                     YMRSGNNQKHEHTFQIGAIGIDAASEGPFKNNSNSSYLLNYRYSTLGLLAPLLPEEAA
                     GTTYQDLSFKLNFPTRRNGTFSLWGIGLLDHSDDTAKQNIADWKYDTDKDNQDVNQYI
                     GALGLSHKILFNHKQYLKTTIAARTNGIDLHTESLDSTLTASPKDVIKNQNYSLILSS
                     FLNTKMNAWHTNRSGIVITSLNYRMNLKKAEGNGGLLRDLVNENGGSFLVSAFTNSML
                     NINDRIKLNAGVTSQWFSLNDTYTIEPRVGIKCQYSPKQALSFAYGLHSRLERLNYYF
                     ITDKQGNFVNNDLGFSRVHHLVLGYDLNISEFLHLKIESYYQHLFGLPVEANSTFSLI
                     NQQNDWFVDKQLTNEGTGRNYGIDITFEKYMSQGYYYLVTASVFNSEYKAADKLWRNT
                     RFNRNYALNFLVGKKWLLGKDKRKVLGANVRFSLQGGDHYSPVDVMQSITQLDVVFNE
                     NLAFSKQFEPSFTSHFTASYRINKKNTAHEFALKIINLTQYKEIAGFKFNHQTQMVDT
                     IRDPIFIPNFSYKIDF"
     gene            complement(3122..3619)
                     /locus_tag="PLE23_08330"
     CDS             complement(3122..3619)
                     /locus_tag="PLE23_08330"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMX83307.1"
                     /translation="MPSKFIVLVLLMCSINILAQQPTQNIRRIVLDEASNAPLAYATV
                     GLANTSFGNLTDSLGHFLLKDVPTGRYDVYVTYLGYEPVVLKEIEVTSAKEILLNIQM
                     KENVNKLDEVVIKSKINKEQPLNTMATVSARMLSVEEARRYAGGFDDPDPFGFGFCRC
                     VKQFW"
     gene            complement(3697..4476)
                     /locus_tag="PLE23_08335"
     CDS             complement(3697..4476)
                     /locus_tag="PLE23_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HMX83308.1"
                     /translation="MTEEDVINFNNAYLQIMSQQTFETEKIDYSVIDRHAPVLHSIAR
                     ISNAVVSVFDFSKKQHVFNSSNLGEILGYTPEQVRASGNQFIDSKIHPEDFGMLAQIG
                     LSTFKLFFSFSEDEKLNHKLVSEFRILNAAGKYIRVIEQQHVLELDHLGNLWLALSIL
                     DISPNQQNLNEGLKSELINFRTGKIILINRMPDELAVQLTRREKEILNLVKSGLLSKE
                     ISDKLAISLHTVNTYRQKVIGKLGVNNSMEAVMLATRLGLI"
     gene            complement(4554..5033)
                     /locus_tag="PLE23_08340"
     CDS             complement(4554..5033)
                     /locus_tag="PLE23_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX83309.1"
                     /translation="MKNNKPLALVCKRLSEVNSDFEELVMLLNQELAIINGDKNDFFI
                     QFNNIDQLRHIVIAYQSGRAVGCGAMKVYGDNMMEIKRMYVRQPYRGKGVASTILSEL
                     EKWAVELGTHRCILETHERMKDAIALYKKNQYAEIERYGQYKDEPNSVCFEKCFNTR"
     gene            complement(5020..5787)
                     /locus_tag="PLE23_08345"
     CDS             complement(5020..5787)
                     /locus_tag="PLE23_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX83310.1"
                     /translation="MMATLYHLNCVRIETPIQANVCGHCLLINEAGRLILIDTGIGLL
                     DTQDPEGRIGKDLINMVGYQLDESITAIKQIVKLGLDPAMVTDCIITHLDNDHTGGLA
                     DFPLATVHIGAEEMANYESGNLRYLKMPLAHLPKIKTYNTNDTEWFGLQARKIAIGTE
                     TEIFLVPLFGHTLGHCGIALRVSDRWLFYIGDAYYLRIELTDNEHPVNQLAQMRADDN
                     EIRLESLEKIRKLKATHPEIDLYGYHDIDEFDYFEKQ"
     gene            complement(5963..>6369)
                     /locus_tag="PLE23_08350"
     CDS             complement(5963..>6369)
                     /locus_tag="PLE23_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HMX83311.1"
                     /translation="DIGFEAYTKRDIRRIILKDADTLVYDKKYADRSQLNILTNGLLP
                     EEDVLIIKNGLVTDTSYCNVAFTDGAKWYTPNMPLLHGTQRAYLLRKKMIHPKRISVL
                     DISSYSHIRLFNAMIPWDEAIELEVEESIVRM"
BASE COUNT         1752 a         1367 c         1129 g         2121 t
ORIGIN      
        1 cttttgccgt gacgctggtc tgcttgctga acagaaagat acaaaagtat ccactatacc
       61 actatgccgt actagatgga acgatgctgt tattaggctt gctggttata ggctttcatt
      121 atcttgatat aataatataa acacagatac cgaattaggc tggttactac aaatatttat
      181 tacttcaaat ccatttcaaa tgctcaaaaa aagtagttca cgataattat atcacttttt
      241 tatggatttc ttgttctgat cattttcgtt caaattccca atgcgttgtc tgggttagat
      301 tcgtatgttg accgatacat tgtctccaca ttgtaccttc tccttaagcc taattgaagc
      361 tttaatgggt aacaggtaac attgctgtcg ggtgtcatac cagatggcag tatcccattc
      421 gctgctacca agctgtgccc ttgcctttaa ccttccccaa ccttcctcca gatgttggtt
      481 gaacaatcta atctcagctg atagttcatc tggcaatgca acaaaatacc accctgcact
      541 acctttgtgt ctccataact cagcttcgaa attgtacctc agattcatta tacaacaggt
      601 tttttgttta atgccagaga ttgtgtcgct tctattattc tcttctggcg tgtttctggt
      661 cgttttgcca tggctattct ttgcaacact acttttttaa gtgacttact aagtgtatca
      721 taaaattgca ttgctgcggg agaaagcgtg aaggcttgca tcaaatcttc aggtatcatc
      781 agttgttcta cttcatccaa tattgtccag ctgccattgt tccttgccct ttccactgct
      841 tccataccgg caatggtcat ccttccttct gctatcagtt tttcaatctt ttctttgttg
      901 atcatcgacc aggtactatc gggttttctt tgactgaata attgtcggta tctgtgttca
      961 tcaattgttt tgcgtgtgct gtcaatccat ccaaaacaca atgcctcatc caccatttgg
     1021 ctccaaccta cagaaggttt cactgacttc ttggtgtggc aaacaaccca aacagagtgt
     1081 tcagatcggt ggtttgcctc gagccagctg cgccattcat caacagagaa aatttcaata
     1141 gaactaaggt cttctaagag catacccgaa tttttatcca aaaatatcaa atcatcttca
     1201 actgtccact cctttcaatt agaatacttg aaaaatttct gctaatcaat ttttcagaaa
     1261 actatatgta ctatagattg tgtggaaaat ttaaaaatca atcttgtaac tgaagttcgg
     1321 aataaatata gggtcgcgta tcgtatctac catttgcgtt tggtggttaa acttgaatcc
     1381 tgctatctct ttatattgtg tcaggttaat tatttttagg gcaaattcgt gtgctgtatt
     1441 ttttttattg attctgtagc tcgcagtaaa atgactggtg aaggaaggtt caaactgctt
     1501 ggagaatgcc agattttcat taaatactac atccagttgt gttattgatt gcataacatc
     1561 caccggcgaa taatgatcac ctccttgcag actgaatctt acatttgcgc ctaacacctt
     1621 tctcttgtct ttgcctaata gccatttttt acctaccaaa aaattgagag cgtagttgcg
     1681 gttaaatctg gtatttcgcc acaatttgtc tgcagcttta tactccgaat tgaagaccga
     1741 tgctgtgacg agatagtaat agccttgcga catatatttt tcaaacgtta tgtcaatgcc
     1801 gtaatttctt cccgtacctt cattcgttaa ttgtttgtcc acaaaccaat cattttgctg
     1861 attgataagc gaaaaagtac tgttcgcttc aactggcaaa ccaaacagat gctggtagta
     1921 actctcaatt ttcaaatgta aaaattcgga aatattcaag tcgtaaccca ataccaaatg
     1981 atggactcgt gaaaaaccaa ggtcgttatt gacaaaatta ccttgtttgt ctgtaataaa
     2041 ataataattg agtcgctcca atctgctatg aagtccgtaa gcaaaactaa gtgcctgttt
     2101 cggcgaatat tggcacttga tacctacacg tggctcaata gtatatgtat cattgagcga
     2161 aaaccactga cttgttacac cggcattcag ctttattcgg tcattaatgt tcagcattga
     2221 gtttgtaaat gccgaaacca ggaagctgcc accattttca ttgaccaaat ctctgagcaa
     2281 tccgccattt ccttctgctt ttttcaagtt catcctgtag ttcaggctcg tgataacgat
     2341 accacttctg ttggtatgcc aggcattcat tttcgtattc aaaaaagaac tcagtattaa
     2401 actgtagttt tggtttttga tgacatcttt tggtgaagct gtcaaggtac tatcaagact
     2461 ttctgtgtgc agatcaatgc cattggtcct tgctgctatg gttgttttga gatattgttt
     2521 gtggttgaac agtattttgt ggcttagacc taatgctcct atgtattggt ttacgtcctg
     2581 attatccttg tctgtatcgt atttccaatc tgcaatattc tgtttggcag tgtcatccga
     2641 atgatcaagc aagcctatac cccataatga gaaagtccca tttcgcctgg tcgggaaatt
     2701 cagcttaaaa gaaagatcct gatacgtagt accggcagct tcctccggca acaatggggc
     2761 aagtaaacca agggtcgaat atcggtaatt gagcaaatat gaactgttgc tgttgttctt
     2821 gaacggacct tctgaagcag catcaatacc gatggcacca atctgaaaag tgtgttcatg
     2881 cttctgatta ttacccgaac gcatatatat atcgaacact ccagacagag cattgctata
     2941 ctctgccggc atggcgccag agaaaaaatc tgaatttgcc aataattggg cactcagggc
     3001 ggtaatacca cctccaccaa atgccgccag gtctgcaaaa tgatttgggt tagagatttc
     3061 aactccttcg agcttccatt ggagcgactg agggctattg ccccttacta cgatagcatt
     3121 gttaccaaaa ttgcttgaca caccggcaaa agccgaaacc aaacgggtca gggtcatcaa
     3181 atcctcctgc ataacgcctg gcctcttcaa cactcaacat tcttgcactc accgtagcca
     3241 tagtgttgag tggttgttct ttattaatct ttgacttgat gactacttca tccaatttat
     3301 ttacattttc cttcatctgg atattaagca gtatctcttt tgccgatgtc acctctattt
     3361 ctttgagaac aacaggctca tagccgagat atgtaacata aacatcataa cgcccggtag
     3421 gtacatcttt gagcaaaaaa tgacccaagc tatctgtaag atttccaaaa gatgtattcg
     3481 ccagacctac agtcgcatag gccaatggag cattggaggc ttcatccaga acgatacgtc
     3541 tgatgttctg ggtgggctgt tgtgccaata tgttaataga acacattaat agaaccaata
     3601 cgatgaactt tgacggcata aatttttatt tgaatataaa tacaaagttg aatcaagtgc
     3661 cgtctaaaaa aaatactgat ttatcagtat aatgccctaa atcaaaccca acctcgtggc
     3721 aagcattact gcctccatcg aattgttgac accaagtttt ccaatgactt tctgtcggta
     3781 agtattgaca gtatgcaggc ttatggctaa cttgtctgag atctccttgc ttaataatcc
     3841 actcttaacc agatttagta tttccttttc tcttcgggtc aattgcacag caagttcatc
     3901 cggcatccgg ttaatgagta tgatcttacc tgttctgaaa ttgatgagtt cactttttaa
     3961 tccttcattt aggttttgtt ggttaggtga aatatcaaga atgctcaatg ccaaccaaag
     4021 atttccgagg tggtcgagtt ccaaaacatg ttgttgttct attacccgaa tatatttacc
     4081 tgctgcattc agtatcctga actcactcac gagcttgtgg ttcaattttt cgtcttctga
     4141 aaaactgaag aaaagtttga atgtagaaag cccgatctga gcaagcattc caaaatcttc
     4201 cggatgtatt ttcgaatcaa taaattggtt tccacttgcc cgaacctgct ctggcgtata
     4261 tccgagtatc tcgccaagat ttgaagaatt gaacacgtgc tgtttcttgc taaagtcaaa
     4321 aacactcact accgcattac ttattcttgc aatactgtgc agtaccggtg catgtctgtc
     4381 gattaccgaa taatcaattt tttcggtttc aaatgtttgt tgcgacatta tctgcagata
     4441 tgcgttgtta aagtttatta catcctcttc ggtcatctgt ggttttgata tttgtttgtt
     4501 gtaatgttgg ttttagctat tagaatgcag ataaatattc accaaaattg tgcttatctt
     4561 gtattaaaac atttttcaaa gcaaacacta tttggttcat ccttgtattg gccgtatctt
     4621 tctatttcag catactgatt ttttttatac aatgcaatgg catctttcat tctctcatgt
     4681 gtctcaagaa tacatcgatg agtacccaac tcaactgccc atttttctaa ctctgacaga
     4741 attgtagaag ctacaccttt gccacgataa ggctgcctta catacattct cttgatttcc
     4801 atcatattat caccatatac cttcattgca ccgcaaccta ctgccctacc cgattggtat
     4861 gctatgacga tgtgcctgag ctggtcaata ttgttaaact gtatgaaaaa atcgttcttg
     4921 tctccgttaa taatcgccaa ctcctgattc aaaagcatca cgagttcctc aaaatccgaa
     4981 ttgacttcgg ataatctttt acagactaat gcgagtggtt tattgttttt caaaataatc
     5041 aaattcgtca atatcatggt atccgtataa atctatctcc ggatgcgttg ctttgagttt
     5101 tctgattttt tcaagacttt ccagtcgtat ttcattatca tctgcacgca tttgtgccag
     5161 ctgattgaca gggtgctcat tgtccgttaa ttcaattcgt aaataatacg catcacctat
     5221 ataaaacaac catctgtcac tcactcgcaa tgctatgcca caatggccga gcgtatgccc
     5281 gaacagagga accaggaaaa tttccgtctc cgtaccaatc gctatttttc ttgcctgcag
     5341 cccaaaccat tctgtatcgt tcgtattata cgtttttatt ttaggtaaat gagccaaagg
     5401 catcttcaaa tatctgagat tgcctgattc atagtttgcc atttcttctg ccccaatatg
     5461 cactgtcgct aaaggaaaat cggcaagacc accggtgtgg tcattatcaa gatgtgtaat
     5521 gatacaatct gttaccattg ctgggtctaa gccaagcttt acgatctgct taatagctgt
     5581 tatgctttca tccaactggt accctaccat atttataaga tctttaccga ttctcccttc
     5641 agggtcctga gtgtctaaca gaccaatgcc tgtatcaatc aatatcagcc gccctgcttc
     5701 atttatcaac aaacaatgtc cgcaaacatt agcttgtatg ggtgtctcaa tccttacaca
     5761 gtttaaatga tataatgttg ccatcataac tttttagcat tacttttaag gtgtaaaaat
     5821 atacttttcg agctgtggtt cagtcattga gtgaataaaa caatttacag acttttactt
     5881 tatccttgta aaacattttc cgaccaccaa aattatgtta aagatgactg taaaaataag
     5941 caaggattgt agcattttat tattacatac ggacgataga ttcttccact tctagttcga
     6001 tggcctcgtc ccacggtatc atggcattga acaatcggat gtgactgtaa ctggaaatat
     6061 ctagtactga tattctcttt ggatgtatca ttttttttct tagtaaatat gctctttgtg
     6121 taccatgtag caatggcata ttgggtgtgt accattttgc tccgtcggta aaagctacgt
     6181 tgcaatagct cgtatctgtc accagtccgt ttttgatgat cagtacatct tcttcaggca
     6241 gcaatccatt tgtgagtata ttcagctgcg agcggtcggc gtacttttta tcataaacaa
     6301 gcgtatcagc atctttcagt ataatacgcc gaatatctct ttttgtataa gcttcgaacc
     6361 caatatcat
//
LOCUS       DAOQQY010000161         6335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5263_length_6335_cov_5.822771, whole genome shotgun sequence.
ACCESSION   DAOQQY010000161 DAOQQY010000000
VERSION     DAOQQY010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_6335_cov_5.822771"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="PLE23_08355"
     CDS             <1..492
                     /locus_tag="PLE23_08355"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83312.1"
                     /translation="LMGGLTICNPATHISYKKLHTPAGIYMVLISPENETPKIDFDAT
                     LNTQLTFDKTTAQQAALGSFITAMYTTDIPLLNECMSDYIVEPYISGILPDFNELKKI
                     AKEENGSGFGITGRGPAMFAVCDNSLKAENIGKKAEMYFANTKRKAKCNISKINLQGT
                     VLF"
     gene            527..1114
                     /locus_tag="PLE23_08360"
     CDS             527..1114
                     /locus_tag="PLE23_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HMX83313.1"
                     /translation="MNSNHSERIRAIISESIRVKENILADADFLSIIDKVVKVVTSAF
                     SQGKKVLFCGNGGSAADAQHLAAEFSGRFYKNRHPLFSEALHVNTSYLTAVANDFSYD
                     EVYARLVQAMGTTEDVLFAISTSGNSPNILRAIEEARLKGMTVVGLTGDKGGKMKEMC
                     DYIFCVPSADTPRIQESHIMIGHIICYLVEDNLFG"
     gene            1114..1659
                     /locus_tag="PLE23_08365"
     CDS             1114..1659
                     /locus_tag="PLE23_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HMX83314.1"
                     /translation="MNIEQFKDWALFLDRDGVINRRIPGGYVMHIDDFIFNPGAREAI
                     AIFSGVFSHIFVVTNQQGIGKGMMNETDLNIIHDYMLTHINKTGGRIDKIYFCPGLAK
                     DKPLCRKPQPGMALQAKADFPDIDLSKSLMIGDSVSDILFGNNVGMLSFFINNTGEIE
                     DDLPSGTPIYTSLAEIAGIKI"
     gene            complement(1666..4110)
                     /gene="priA"
                     /locus_tag="PLE23_08370"
     CDS             complement(1666..4110)
                     /gene="priA"
                     /locus_tag="PLE23_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HMX83315.1"
                     /translation="MQTRYVDVLIPLAIGKPLTYAIPATLEPHVAFGCRCEVQVGKSK
                     LYSGIITAVHNNKPAYRTKEILSVFDEKPIITPKQWMFWQWMAEYYCCTVGEVMSAAL
                     PAHLKLASETKLLLNKLFDDQLPELDDKEYLICEALQVRNSITIDEVRAILDQKNVYP
                     VINKLIERGIVISEEEIKEKFKSQKFNFVRLCEPYRSDPDTLKYAFELTAKSEKQTAA
                     ILAFWQKSQGNGMVKLPDLIKEYNVSREVVYTLVKKNIFEIVERETSRILSDTTPDYD
                     DVALNDVQLTAFEQLKESFEKQDVALIFGVTGSGKTLVYTEYIKEILKDGGQALFLLP
                     EIALTSQLVARLKLLLGREVVVYHSRMSNQERVEVWNAVLAGREVILSARSGIFLPFK
                     NLKCVVIDEEHDPSYKQNDPAPRYQGRDAAIYLSYLHEAKAIAGSATPSVETYNNAVT
                     GKYGLITMKERFGGIRLPEFEIVDLTAESKKRKLQAHFSERLIALLKENLNNNKQAIL
                     FQNRRGYAPVIECPNCDWAMECPNCDVHLTFHKITKTMRCHYCGYSTKLPHECPACAH
                     PSLEIKGIGTERIEDELQTYLPEARISRIDMDVARQKHSISKILADFEDRQTDILVGT
                     QMITKGLDFDHIGVVGIINFDQLYRFPDFRSQERAFQLVTQVAGRAGRKNEQGKVILQ
                     TFHTGHPLIKRIITYDYEGFFLTETEERKKFFYPPFCKMIYINIRHKDFKVTEQSAKF
                     LVGKLTEKYSNRIRGPVEPGIPRVRNQFIREIWIKSERKPEVVKAIKTDIIQFVALTQ
                     QQKGWSNVRITIDVDP"
     gene            4357..5862
                     /locus_tag="PLE23_08375"
     CDS             4357..5862
                     /locus_tag="PLE23_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMX83316.1"
                     /translation="METKTTTSTPTALESYFRPYRNNVVGIDTYFHTPEGKKQMLYSD
                     WTASGRLYRPIEEMLLNEIGPLVANTHTETNYSGSMMTNAYHKALQIIKKHVKAAPYD
                     VIISEGSGMTGVINKLQRILGLRIHEKFQDKIHIAPEDRPVVFCTHMEHHSNQTSWLE
                     TICDLEIIQANAEGQVCLNHLQELLNKYATRTTKIAAVTSCSNVTGIMTPYHEIAALM
                     HQHGGLCFVDFACSAPYIDIDMHPKDELQALDAIYFSPHKFLGGPGTTGILIFNSKLY
                     TNRIPDNPGGGTVDWTNPWGGHKYVDDIEAREDGGTPAFMQTIKAAMCIKLKEEMRVE
                     KILAREHEILDILWSKLCDVPNIHILAENIKDRLGVISFYIDGLHYNLGVKLLNDKFG
                     IQVRGGCSCAGTYGHILLEVDQNFSRIITDQISVGDLTNKPGWIRMSVHPVMTNEEVH
                     YIADSIVKLAEKHKEWAKDYIYIRRNNDFKHREFGEKDSEMVEGWFNKKLC"
     gene            complement(5922..>6335)
                     /locus_tag="PLE23_08380"
     CDS             complement(5922..>6335)
                     /locus_tag="PLE23_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83317.1"
                     /translation="DSIFLGYIFGGIRSTADKVFWINTGEESFASNRIYKVWLHPKST
                     KSIQPISAAYDYFRPMVYPDEAFANYIIEFSAGDKENISADIRDSQGNVVVQQSMKAK
                     KGVNNMHIPIAKLPEGNYIISINNGKLSHSLKILK"
BASE COUNT         1845 a         1353 c         1340 g         1797 t
ORIGIN      
        1 ctgatgggcg gacttactat ctgcaatcct gccacacata tatcatataa gaagctgcat
       61 acacctgccg gcatctacat ggtactcata agtccggaaa atgagacccc aaagatcgat
      121 tttgatgcga ccttaaatac ccagcttaca tttgacaaaa caactgcgca acaagcggct
      181 ctgggttcat tcattactgc tatgtacaca acagacattc cgttactcaa tgaatgtatg
      241 tcagattata tcgttgaacc atatatttca ggaatccttc ccgatttcaa tgaattgaaa
      301 aaaattgcga aagaagaaaa tggatcggga ttcggtatta cgggtagagg ccctgctatg
      361 tttgccgtgt gcgacaatag tctgaaggca gaaaatatcg gtaaaaaagc agaaatgtat
      421 tttgccaaca ccaaacgtaa ggcgaaatgt aatatttcaa aaatcaacct gcagggaacc
      481 gtactgtttt aaccggtgtt atttaatttt cgattaactt tacatcatga attcaaacca
      541 ttcagaaagg atcagggcga ttatcagtga aagtatccgt gtgaaggaaa acatccttgc
      601 cgatgcagat tttttaagca tcattgataa ggtcgtcaaa gtagttacca gtgcttttag
      661 ccaagggaaa aaagtactct tctgtggcaa tggtggctct gctgctgatg ctcaacatct
      721 ggctgcggag ttttccggaa ggttctataa aaacaggcat ccactgtttt ctgaagcact
      781 ccatgtgaat acttcctacc tgacggctgt ggcaaatgat ttcagctacg acgaagtgta
      841 tgcacgtctt gtgcaggcaa tgggtacaac agaagatgta ttgttcgcca tatcaacaag
      901 cggcaactcg ccaaatatac tccgcgccat agaagaagcc aggctcaaag gaatgactgt
      961 ggtaggattg accggtgata aaggtggaaa aatgaaggaa atgtgcgact atatattctg
     1021 tgtgccatct gcagacactc cacgcatcca ggaatcgcac attatgatcg ggcacatcat
     1081 atgttatctt gtagaggata atctattcgg ataatgaata ttgagcagtt taaagactgg
     1141 gcattgttcc ttgacagaga cggagtgatt aaccggcgca taccgggcgg ttatgtaatg
     1201 cacattgacg atttcatatt taacccgggt gcacgggaag ctattgccat attttcagga
     1261 gtattcagcc atattttcgt agtaactaac caacaaggta ttggcaaagg catgatgaac
     1321 gaaacagacc tcaacatcat acacgattat atgctcactc atatcaacaa aaccggtggc
     1381 cgtatcgata aaatttactt ctgccccggt ctggctaaag acaaaccgct atgccgaaaa
     1441 cctcaacccg gtatggcact tcaggcaaaa gctgattttc ctgacatcga tcttagtaaa
     1501 agtctgatga ttggcgattc tgtgtcggat atattatttg gaaataatgt gggaatgtta
     1561 tcctttttca taaataatac tggtgaaatt gaggacgatt tgccatccgg cacacccata
     1621 tacacttcct tagccgagat agcaggtatc aaaatctgac cgccgttatg gatcaacatc
     1681 gatggtgatg cgtacatttg accagccttt ttgctgctgg gtcaaggcaa caaactggat
     1741 gatatcagtt tttattgctt tcactacttc gggtttgcgt tctgatttaa tccatatttc
     1801 gcggataaac tggttgcgca cacgtggtat gccgggttcg accgggccgc ggattcggtt
     1861 ggaatatttt tctgttaatt ttccgacaag aaattttgct gactgttcgg ttactttgaa
     1921 atccttatgc cggatgttta tgtaaatcat tttacagaaa ggcggataga agaatttctt
     1981 tcgttcttca gtttcggtca ggaaaaatcc ctcataatcg taggttataa ttcgtttgat
     2041 gagtggatgc ccggtatgga aagtctgtaa aatgacctta ccctgttcgt ttttacgacc
     2101 agcccgtcct gccacctgtg tcaccagttg aaatgcacgc tcctgactgc ggaaatccgg
     2161 aaagcggtaa agctggtcaa agttaatgat acctactacg ccgatgtgat caaaatcgag
     2221 gcctttggtt atcatttggg tgcctacgag tatatccgtc tgccggtctt caaaatctgc
     2281 cagtatttta ctgatgctgt gtttctgtct ggctacatcc atatcgatac gacttatcct
     2341 agcctccgga aggtatgttt gcagttcgtc ttctatcctc tctgtgccga tacctttgat
     2401 ttcgagcgac gggtgtgcgc atgccgggca ttcgtgaggt agtttggttg aatagccgca
     2461 atagtggcag cgcattgttt tcgtaatctt gtgaaatgtc aggtgtacat cacaattggg
     2521 gcattccata gcccaatcgc agttagggca ttcgataaca ggtgcgtaac cacgtctgtt
     2581 ttgaaacagg attgcctgct tattattgtt taaattttct ttcagcaatg caatcagtct
     2641 ttccgaaaaa tgtgcctgta gtttacgctt tttcgactca gctgtaagat ccactatttc
     2701 gaactccggc agcctaatgc cgccaaatct ttctttcatg gtaatcagac cgtattttcc
     2761 ggttactgca ttgttgtaag tttcaacaga gggtgtagca gagccggcta tagcctttgc
     2821 ctcatgaagg taggacagat aaatggctgc atctctcccc tgataacgag gtgccggatc
     2881 attttgctta taggaaggat cgtgctcttc atcaatgact acacatttga ggtttttgaa
     2941 tggcagaaaa atgcccgaac gtgcactgag tattacttcg cggccggcaa gtaccgcatt
     3001 ccacacttct actctttcct gattactcat tcgcgaatga taaaccacca cctccctgcc
     3061 gagtaaaagt ttgagccttg ctactagctg actcgtaagc gctatttcgg ggagaagaaa
     3121 aagtgcctgg ccgccatcct taagtatttc ctttatatat tcggtatata ccagggtttt
     3181 gccgctacct gtcacgccga atatcagtgc aacatcttgt ttttcaaaac tttctttcaa
     3241 ttgttcgaat gctgtgagct ggacatcgtt cagggcgaca tcgtcataat ctggcgttgt
     3301 gtcagacaaa atcctgctgg tttcacgttc aactatttca aaaatatttt ttttaacaag
     3361 agtatataca acttcgcgcg acacattgta ttccttgatc agatccggca atttgaccat
     3421 accgttccct tgggatttct gccagaaggc aagaatggct gctgtttgct tttcagattt
     3481 tgctgtcaat tcgaaggcgt atttcaaggt atcggggtcg gagcggtaag gttcacacag
     3541 cctgacgaag ttaaattttt gagacttaaa tttttctttt atttcttctt cagaaatgac
     3601 tattccacgt tctatcagct tattgattac cggatataca tttttttgat ccagaatggc
     3661 gcgcacttca tctattgtga tagaattgcg tacttgcaag gcttcgcaga tgaggtattc
     3721 tttatcatca agctccggta gctgatcgtc aaataattta ttgagcaata attttgtttc
     3781 gctagccagc ttcaggtgtg ccggtagtgc agcgctcatc acctcaccga ccgtacagca
     3841 gtaatattct gccatccatt gccagaacat ccattgctta ggagtaatta tcggtttctc
     3901 atcaaaaacc gacaatattt ctttcgtgcg gtaagcaggt ttattattat gaacggcggt
     3961 aataatgccc gaataaagct tgctcttgcc tacttgtact tcacatctgc acccgaatgc
     4021 cacatgaggc tccagagttg ccggaatggc ataggtcagc ggtttgccta tggccagcgg
     4081 aatcaataca tcaacgtagc gagtttgcaa cagaaataag tttgaggtca aatatacaag
     4141 ctaaaaagcg aattgtgtga aattaaaatt tttctcttgt ggaggtaaaa aatccgctcc
     4201 ctcgcatagc cgaataccga tattaaacac agatattgga aacaacaaaa actacaacaa
     4261 acaagatttc caaaaagttg aacaacaagt gtaatatccg gcatttcgcg aacattttca
     4321 aaatacaaaa tcacgaaaaa agcctaactt tgccttatgg aaacaaagac aacaacaagc
     4381 accccaaccg cattagaatc atatttcagg ccttaccgaa acaacgttgt aggtattgat
     4441 acatattttc atactcctga aggaaaaaaa caaatgttgt acagcgattg gacagcaagt
     4501 ggaagacttt atcgaccaat cgaagaaatg ctcctgaacg aaattggccc cttggtagcc
     4561 aacacacata ccgaaaccaa ttattcgggg tcaatgatga ccaatgctta tcacaaagct
     4621 ttgcaaatca tcaaaaaaca tgtaaaagca gccccttacg acgtcataat atcagaaggt
     4681 agcggaatga ccggtgttat caataagctt caacgaatac tgggattgcg tattcacgaa
     4741 aaatttcagg acaaaataca catagcaccg gaagaccgac cggtagtatt ctgtactcac
     4801 atggaacacc acagcaatca gacaagctgg ctcgaaacca tctgcgacct ggagattata
     4861 caagccaatg cagaagggca agtatgcctg aaccaccttc aggaactact caacaaatat
     4921 gctactcgta ccacaaagat agccgcagtc acttcatgct caaatgtaac cggcatcatg
     4981 accccttatc atgagatagc agcccttatg catcagcatg gtgggttatg ttttgtagat
     5041 tttgcttgtt cggcacctta catagacatc gacatgcacc caaaagacga actgcaggca
     5101 cttgatgcca tatatttttc gccacataag tttctgggtg gcccgggtac aaccggcatc
     5161 ctgatattca acagtaagct ttataccaac cgcatacccg acaatccggg aggaggcact
     5221 gttgactgga ccaatccgtg gggtggccac aaatatgtag atgatataga agcaagagaa
     5281 gatggtggta caccggcatt tatgcaaaca attaaagcag ccatgtgcat taagctcaag
     5341 gaagagatgc gtgtagaaaa gatccttgca cgtgaacatg agattcttga tattctgtgg
     5401 agcaaactgt gtgatgtgcc gaatattcac atactggcag aaaatataaa ggatcggcta
     5461 ggtgtcattt cattttacat cgacggacta cattacaacc ttggtgttaa gctgttgaat
     5521 gacaaattcg gcattcaggt acgtggagga tgttcgtgtg caggtaccta tggacatatc
     5581 ctacttgagg ttgaccagaa ttttagcaga ataattaccg accagataag cgtaggcgac
     5641 cttaccaaca aacccggctg gatcagaatg tcggttcatc cggtaatgac caatgaagaa
     5701 gtacattata ttgctgactc gattgtaaaa ctcgctgaaa aacataagga atgggcaaag
     5761 gattacattt atattcgaag aaacaatgat tttaaacacc gcgaatttgg cgaaaaagat
     5821 tctgaaatgg tagaagggtg gtttaacaag aaactgtgct aataaaattc cataaaaaga
     5881 tcatatcaaa cacagtcctc gtctcgtgct cgtactcttg cttacttaag tatctttaga
     5941 ctatgactaa gcttaccgtt gttaatactt atgatatagt taccttcagg tagcttagct
     6001 attggaatgt gcatattgtt aactcccttt tttgccttca tgctttgttg tacgactaca
     6061 tttccttggg aatctctaat gtctgctgat atgttttcct tatcgcctgc actgaactca
     6121 ataatataat tggcaaaagc ttcatctgga tataccatcg ggcgaaaata atcataagcg
     6181 gcagaaatgg gctggatact cttggttgac ttagggtgga gccatacttt gtagatacgg
     6241 tttgatgcaa agctctcttc gccggtattg atccagaaaa ccttgtctgc ggtactcctt
     6301 attccaccaa aaatatagcc cagaaaaata ctgtc
//
LOCUS       DAOQQY010000162         6322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5280_length_6322_cov_6.905058, whole genome shotgun sequence.
ACCESSION   DAOQQY010000162 DAOQQY010000000
VERSION     DAOQQY010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6322
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5280_length_6322_cov_6.905058"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLE23_08385"
     CDS             <1..285
                     /locus_tag="PLE23_08385"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMX83318.1"
                     /translation="SNMPITTHKNEMNAQIVHLLGGRTYMSGEGAVKNYQDVLIFKNA
                     GIDVRHSEYLGLLHSKPYSRYPAAGRSGIFDILFTIGVDGAREYIMPDDK"
     gene            complement(344..922)
                     /locus_tag="PLE23_08390"
     CDS             complement(344..922)
                     /locus_tag="PLE23_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX83319.1"
                     /translation="MQVSMLQDQDLIKLYLTGNQKAFETLLERHKDKIYTSIYLFVKD
                     TAVAEDIFQEVFIKIINTLREEKYNHEGKFIQWAMRIAYNMCVDYFRRNKRRANISPT
                     DSFDIFDILPQNDLNAEQDIIRNEMHDKLRSIIDKLPAEQREVVILRHYADMSFKEIS
                     QLTRVSINTSLGRMRYALINIRKKLEEKEIAA"
     gene            1474..3672
                     /gene="recQ"
                     /locus_tag="PLE23_08395"
     CDS             1474..3672
                     /gene="recQ"
                     /locus_tag="PLE23_08395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HMX83320.1"
                     /translation="MVEVHDNLTKQLQHFFGFDSFKGNQEAIIKSVLDRQNTFVIMPT
                     GGGKSLCYQLPALLMEGTAIIISPLIALMKNQVDSIRGYSSEDEVAHFLNSSLSRTQT
                     KQVKADISDLKTKMLFVAPETLTKEETLDFLKRIDISFVAVDEAHCISEWGHDFRPEY
                     RRIRTMIDIINKEIPIVALTATATPKVQSDILKILNMENVNSFVSSFNRENLFYEIKP
                     KLKKDQTIKAILSVINEARNQSGIIYVQSRKSAEELAQVLSVNGVKALPYHAGLDPKT
                     RSNTQDAFLMQDIDVIVATIAFGMGIDKPDVRFVIHYDIPKSIENYYQETGRAGRDGL
                     DGKCVAFYSDKDITKLEKFLRDKPVAEREMGAQLLQEVMAYSETSACRRKFLLHYFGE
                     EYQEERCGKMCDNCLHPKEKIEVQKEMQQALNLINFMGEKFVHKSVVDFIMGIETKEM
                     RDYKYTEAKNFGFGKEKDENFWSSILRQALLSNLIYKDIEQYGLLKFTEQGKNYIKHP
                     VSFTMAINQKFEGGDFDPEDSFDKTATLDEELLKILTAIRLKISKQKSLPPYVIFQDP
                     SLQDMCTQYPITIDDMTMITGVSRGKAEKYGHEFIEAIKKHVEENEIDRPTDFLVKQV
                     ANKSKTKVAIIQGIDRKLSFVDIASTNNLTMDELYEELDAIVASGIKLDINYYVKTNV
                     DEYSKEEIYNYFMDAETDSIRDAYKVLKDDDITIDEIRLMRIKFLSEMAN"
     gene            3674..4093
                     /gene="aroQ"
                     /locus_tag="PLE23_08400"
     CDS             3674..4093
                     /gene="aroQ"
                     /locus_tag="PLE23_08400"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HMX83321.1"
                     /translation="MKIIIINGPNLNMLGRREPDIYGNQSFEEYFQKLTGIFPDLSLD
                     YFQSNHEGELIDCIQRAEVEYDGIILNAAAYSHTSLAIAESLACISKPIIEVHISNIF
                     ARETFRHHSVISLHCKGVIVGLGLDGYRLAVSAIATM"
     gene            complement(4064..4483)
                     /gene="rnpA"
                     /locus_tag="PLE23_08405"
     CDS             complement(4064..4483)
                     /gene="rnpA"
                     /locus_tag="PLE23_08405"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMX83322.1"
                     /translation="MSGFLFPKEERLKSSKTIARLFREGNSEFSYPVKFVFVIQGSSI
                     APGIKVAVSVSGKKFKRAVDRNIIKRRLREAFRLQKSELLDELLSRQILMEGMFIYVA
                     NDIVDYQIIAVAMKKILTGLSKETIQYQSHSGDSTDC"
     gene            complement(4549..4704)
                     /gene="rpmH"
                     /locus_tag="PLE23_08410"
     CDS             complement(4549..4704)
                     /gene="rpmH"
                     /locus_tag="PLE23_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HMX83323.1"
                     /translation="MKRTYQPSKRKRANKHGFRERMSTKNGRKVLARRRAAGRYKLTV
                     SDERGAK"
     gene            complement(4798..6321)
                     /gene="rho"
                     /locus_tag="PLE23_08415"
     CDS             complement(4798..6321)
                     /gene="rho"
                     /locus_tag="PLE23_08415"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017631298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMX83324.1"
                     /translation="MTPPVAVNTNDQTESKQAEPKQDYSYDNRHDKRDKSQQRTERQD
                     RPERNGRQERPERAERQDRPQRTDRDERIERTDRSERKEDSRPRYKEEPKHPVQPELE
                     QVDELETIIASKEAEEAEAREAVTDASKFNVELDGIIEGEGVLEMMPDGYGFLRSSDY
                     NYLSSPDDIYVSPSQIKLFGLKTGDTVVGAIRPPKEGEKYFALLKVSHINGYDPKDVR
                     DRVPFDYLTPLFPNEKFRLTSPSHAKDYGVRMIDLFTPIGKGQRGLIVAQPKTGKTFL
                     LKDLANAISRNHPECYMIVLLVDERPEEVTDMKRSVNAEVVASTFDEPAENHVRVANI
                     ILEKAKRLVECGHDVVILLDSITRLARAYNTVAPASGKVLSGGVEANALQKPKKFFGA
                     ARNIERGGSLTILATALIDTGSKMDGVIFEEFKGTGNMELQLDRSLANKRMFPSVDLT
                     KSSTRRDDLLLEKDTLQRMFILRNHLADMKPEEAFEFLMKHMSGTSTNEEFLISMNG"
BASE COUNT         1909 a         1252 c         1225 g         1936 t
ORIGIN      
        1 tctaatatgc cgattaccac gcacaaaaac gaaatgaatg cacagatcgt tcatttattg
       61 ggtggccgta cttacatgag tggcgaaggt gcggtaaaaa actaccagga tgttttaata
      121 ttcaagaatg caggtataga tgttagacat tctgaatacc tcgggttact ccattccaag
      181 ccatattcaa gatacccggc tgccggcaga tccggtattt ttgatatctt gtttacaatt
      241 ggtgtagatg gagcgagaga atatatcatg cctgatgaca aataaaaaag ccggacattg
      301 ggtaaatatc cggcaataac agttaattat ttttaataat atctcatgct gcgatttcct
      361 tctcttcgag cttcttcctg atattaatca gcgcataacg cattctaccc agagaagtat
      421 tgatacttac gcgagtaagt tgtgaaattt ccttaaagct catatcggca taatgtctga
      481 gtatgaccac ttcacgttgt tcggccggca gtttgtctat tatgcttcgg agtttatcat
      541 gcatttcatt gcggatgatg tcttgttcag cattgaggtc gttctgaggc aagatatcaa
      601 atatatcgaa tgagtctgtc ggagaaatat ttgcccggcg cttgtttctt ctgaaataat
      661 ctacgcacat attataagct atacgcatag cccattggat aaacttgcct tcgtggttgt
      721 atttttcttc gcgtagagta ttaatgattt ttataaaaac ttcctgaaaa atgtcttcag
      781 ccacagctgt atctttcaca aacaaataga tggaagtata aattttgtcc ttatgtctct
      841 ctaaaagagt ctcaaacgct ttttgattac ctgttaaata cagctttatc aaatcctgat
      901 cctgaagcat acttacttgc atgactgaat gtttaaatag taaaaaaata tttagttcag
      961 tgtcgaagtg tagcttatag attcagtttt aaaagagtta aaaaatagat tgaaaaatga
     1021 tttcgtttcg tttgatggga taaaaatata ttgattttgg attaaaatcc agtttgatta
     1081 aaaattttaa cacttcgtta accaattgag acaaaaaata acacttttta gcagaaatta
     1141 tatgtttttt gcaagctgtt tcatttttta tcttgcactt gcactattct tcattccgct
     1201 tgcatcaagt accttagttc atgccatatt gatttatatt tctcaaatat acgaagattc
     1261 tttagctcaa cacttgacaa tagaatcggt attttgtaat ttcgcaggct ttttaaaaaa
     1321 tgatataaaa acatccaaat taaagtaaat gaattttttt ttatgaaata ttgtaagatt
     1381 gtgaaaaata tacattaatt ttagtgacaa attggaaaag tagtttaggg tgtttttttt
     1441 aaaaaaaaat tacttgataa atcaacatgt ttcatggtag aagtacacga taatctcaca
     1501 aagcaactcc aacatttctt tgggtttgat tcattcaaag gaaatcagga agccatcatt
     1561 aaaagcgtgc ttgacaggca gaatacattt gtaattatgc ctacaggtgg tggtaagtca
     1621 ttgtgttatc aattgcctgc tctgcttatg gaaggcacag ccatcattat ttcgccactc
     1681 atagctttga tgaaaaatca ggttgactcc atacgcggct attcctcaga ggatgaagtg
     1741 gcacattttc tcaattcttc attatcacga acacagacca agcaagtaaa ggcagatatt
     1801 tcagatctca agacaaaaat gctttttgta gcaccagaga cactaaccaa ggaagaaaca
     1861 ctcgattttc ttaaaagaat agatatctca tttgtggcag ttgatgaagc acactgcata
     1921 tcggagtggg ggcatgattt caggccagaa tatcgccgca tccgcaccat gattgacatt
     1981 atcaataaag aaatacccat cgtggctttg acagcaacag ctacgcctaa ggtgcagtca
     2041 gatattctga agattctgaa tatggagaat gtcaatagtt tcgtttcttc atttaaccgt
     2101 gaaaatctat tctatgaaat aaagccaaag ctcaaaaaag accaaaccat aaaagccata
     2161 ctcagtgtca tcaatgaagc acgtaaccag tcaggcatta tctatgtaca aagccgcaaa
     2221 tcggccgaag aacttgctca ggtactgagt gtcaatggtg tgaaagccct accttaccat
     2281 gcaggcctcg accccaagac aaggagcaac acccaagatg ctttccttat gcaggatatt
     2341 gacgtcatcg tggctaccat agcattcggt atgggtatcg acaaaccgga tgtccgtttt
     2401 gtgatacatt atgatatacc taaaagcatc gaaaactatt atcaggagac tggtcgggcc
     2461 ggccgagacg gacttgatgg aaaatgtgta gctttttact cagacaaaga cattacaaaa
     2521 cttgaaaagt ttcttagaga caaacctgtc gctgaacgcg aaatgggcgc acaactcctg
     2581 caggaagtaa tggcatactc tgagacttca gcctgtagaa gaaaattcct ccttcattac
     2641 tttggcgaag aatatcagga agaacgttgc ggaaaaatgt gtgataactg cctccaccca
     2701 aaagaaaaaa tcgaggtaca aaaagaaatg caacaggcac tcaacctaat caattttatg
     2761 ggtgaaaaat ttgtacacaa aagcgtggtc gattttatca tgggcatcga gacaaaagaa
     2821 atgcgcgatt acaaatatac cgaagcaaag aattttgggt tcggtaaaga aaaagatgaa
     2881 aacttttggt catcgatatt aaggcaagct ttgttatcaa accttattta caaagatatt
     2941 gaacaatatg gccttctcaa attcacagaa cagggcaaaa actatatcaa acaccctgtg
     3001 tcttttacta tggccatcaa ccaaaagttc gagggcggag attttgaccc cgaagattct
     3061 tttgacaaaa cagctaccct tgacgaggaa ctgctaaaga tacttacagc tatacgccta
     3121 aagatttcaa aacaaaaatc actgccacct tatgtcatct tccaggatcc ttcactacaa
     3181 gatatgtgta cccaatatcc gataacgata gacgatatga cgatgataac cggtgtaagc
     3241 cggggtaagg cagaaaaata cgggcacgaa tttattgagg ccatcaagaa acatgtagaa
     3301 gaaaatgaaa tagaccgccc gacagatttc cttgtaaagc aggttgccaa taagtcgaaa
     3361 accaaggtgg ctatcattca gggcattgac cgcaaattgt cttttgtgga catcgcttct
     3421 accaacaacc ttaccatgga cgaactctac gaagaactgg atgccattgt agcctcaggt
     3481 attaaactcg acattaatta ttacgttaag acgaatgttg atgaatacag taaagaagaa
     3541 atctacaatt acttcatgga tgctgaaact gattctatca gagatgccta caaggtacta
     3601 aaagatgatg acattacaat agatgagata cgcctcatgc gcatcaagtt tttgtctgaa
     3661 atggccaact gatatgaaaa tcatcattat caatggccca aacctgaata tgttgggcag
     3721 gagagaaccg gatatatacg ggaatcaatc cttcgaagaa tattttcaaa aacttaccgg
     3781 aatatttcct gacctaagtc ttgattattt tcaaagtaac catgaaggcg aattgattga
     3841 ttgtatccaa agagcagagg tcgaatacga tggaattata ctaaatgcag cagcttattc
     3901 acacacatct ttagccattg cagaaagcct ggcttgtata agcaaaccta ttatcgaagt
     3961 acatatatcc aacatttttg cacgcgaaac attccgacac cattcagtaa tctcattaca
     4021 ctgtaaagga gtaattgttg gtcttggact ggatggctac cgcttagcag tcagtgctat
     4081 cgccactatg tgattgatac tgtatggttt cctttgacaa tccggtgagg atttttttca
     4141 tagctacggc gatgatctgg taatcaacga tatcatttgc cacatatata aacataccct
     4201 ccatcaatat ctgtctggac aacagttcat ccaacagttc agatttttgc agcctgaatg
     4261 cttcacggag ccgtctttta ataatattac ggtcaactgc tcttttaaat ttttttccgg
     4321 aaacacttac agctaccttt atacctggtg ctatggatga gccctgtata acgaacacaa
     4381 atttgacagg ataggaaaat tcactattgc cctcgcgaaa cagccgtgcg atagttttgg
     4441 atgatttaag tctttcttct tttggaaata aaaaaccgga catataccaa atactttaat
     4501 tgcacagtat gattgtgcaa ttaaagtaaa gttaagcaaa aagtatgatt acttagcacc
     4561 tctttcgtca gaaacagtaa gcttgtatct gccggcagca cgtcttctcg ccaatacttt
     4621 tctaccgttt ttggtactca ttctttcacg aaaaccatgc ttgttggcac gctttctttt
     4681 agatggttgg taagtccttt tcataacaaa ttatatctaa accctttcgg gtaatttttt
     4741 agaagtgcaa aggtatgctt ttattaaaga aaatacaaat aattttggat ttttatttta
     4801 tccgttcata gaaatgagaa attcttcatt cgtagatgta cccgacatgt gtttcatcaa
     4861 aaactcaaaa gcttcttctg gcttcatatc agccaggtga tttctgagta tgaacattct
     4921 ttggagggta tctttttcga gcaagaggtc atcccgtcgt gtgcttgatt tggtaaggtc
     4981 aacagaaggg aacatccgct tattggccag agatcggtct aattgaagct ccatattacc
     5041 tgttcctttg aattcttcaa agataacacc atccattttc gatccagtat ctatgagggc
     5101 tgtcgcaagg atggtcagtg aaccaccacg ttcaatattt ctggctgcac cgaagaattt
     5161 ttttggtttt tgaagggcat tcgcttctac gccacccgac agtactttgc cgctggcagg
     5221 tgctactgta ttgtaggccc ttgccagtcg tgtaatagaa tcgagcaaga tgacaacatc
     5281 atgaccacat tctaccagcc gttttgcttt ctcgaggata atattagcta cacgtacgtg
     5341 attttcggca ggttcgtcaa aggtagatgc aaccacttcg gcatttacac ttcgcttcat
     5401 gtctgtcacc tcttcaggtc tttcatctac aagcaataca atcatgtagc attcaggatg
     5461 atttctggag atggcattag ccagatcttt taggaggaat gtcttaccgg tttttggttg
     5521 cgcaacgata agaccccgct gcccttttcc tataggtgtg aacagatcaa tcattcgtac
     5581 gccatagtct ttggcatgtg agggtgatgt cagcctaaac ttttcatttg ggaaaagtgg
     5641 tgtcagataa tcaaaaggta cacggtctct aacatcttta ggatcatagc cattgatgtg
     5701 cgatactttt aataatgcaa aatacttttc accttccttg ggtggtctta tagcgcctac
     5761 tacagtatca ccggtcttga gtccgaagag tttgatctgt gaaggtgaaa cataaatatc
     5821 atcaggtgaa gagaggtaat tatagtctga tgagcggagg aatccatagc catcaggcat
     5881 catttcgagt acaccttcgc cttcaatgat tccatcaagc tccacgttaa attttgatgc
     5941 atcggttaca gcttctctag cttcagcttc ttctgcctcc ttggaggcaa tgatggtttc
     6001 gagttcatcc acttgttcaa gttctggttg aacaggatgt tttggctctt ctttatatct
     6061 tggtctggaa tcttcctttc tttcggatct gtccgtgcgt tctattcttt cgtcccggtc
     6121 tgtacgttgt ggtcggtctt gcctttctgc tctttccggc ctttcctgtc gtccattgcg
     6181 ctcaggtctg tcctgccgtt ccgttcgttg ttgcgattta tctcttttgt catggcgatt
     6241 atcataagaa taatcctgct ttggttcggc ttgctttgac tctgtctggt catttgtgtt
     6301 gacagctacc ggcggtgtta tc
//
LOCUS       DAOQQY010000163         6313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5284_length_6313_cov_5.448386, whole genome shotgun sequence.
ACCESSION   DAOQQY010000163 DAOQQY010000000
VERSION     DAOQQY010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6313
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5284_length_6313_cov_5.448386"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLE23_08420"
     CDS             complement(<1..275)
                     /locus_tag="PLE23_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83325.1"
                     /translation="MKKTLKKILIIASVIFGILLSAAIILPVIYKDKIKAMVMEDINK
                     NLDAFVTVGDIDLTLISTFPYLGVKFKDISIFGKKEFASVPLFSTKQL"
     gene            354..1559
                     /locus_tag="PLE23_08425"
     CDS             354..1559
                     /locus_tag="PLE23_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83326.1"
                     /translation="MMKPDSINFTWIDTDHKLKDFSKDHTLQQWIAFDTEFIGERRDK
                     TLLCLIQVSSELGIFLIDCIKINDLTPFLNLVINPDILKITHAGENDYKVLFELYRII
                     PVNVADVQIAAGFAGYKFPVSLAGLMSGELNRKLDKSYTVIDWEQRPLDERYIQYAVE
                     DVLFLKQIYDRLLARITKAGMESWIKQEFEFLTLRETYAGSPEKEALAAINLTQLSQK
                     ERILMLRLYQWRYDQAELTGKSKEKILPKKWINILARQLKHGKKHLLSNRSISSGFID
                     AQWTQLELLLNQPTTDDERELVKNATGETPTDPVHESLQDLVMSIIRYQCRLKGVDPG
                     LVMPPHIIKLLKRKVTDASKILETTWRSDLLGDEFLKWIDFENLLSFTFEENKIIIEK
                     NDKNKNLKH"
     gene            1612..3180
                     /locus_tag="PLE23_08430"
     CDS             1612..3180
                     /locus_tag="PLE23_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83327.1"
                     /translation="MRKFILCVFSFCSMAVIIQAQTLNVEWQKCLGESGKEERPLGLT
                     RTDQSVTVMFGTTSTTGDFSGNKGETDWYYTKFDSKQVKKYGKIYGGSKIETLSWINE
                     NSDQTFTVTGITNSNDGNVNNNHGGNDAWAIKLTENGNIQWRKCIGGTGDDQGYFSAE
                     TADKNYLFLGSTTSKDGDFTTNNGGSDIFMIKTDINGIFQFTKLYGGSGNEKPVGMVK
                     LTDGYIILAESNSPSISGAANANKGKTDFLLIKTDLNGNASWAKFFGGPDDDNPAHIT
                     LLSDGNLLVTGTTSSTSGDISGAHGGKDIWVAKISSEGTLLWAKNLGGSGNDSGKMAV
                     EYSYTNPVKYFIIGSTASANGGNINGAKGGSDIWVARLSSTGNFENGKNFGGSKDDFA
                     GSISVMTDNSLYITGESNSTDGDVVGNKGGQDVWIAKLAKPTATFDIPQSFDLKVFPN
                     PAATSIHVKWDDQTPVELLDITSITGVSMMQIPVDKSSGFTTIDLGNIPNGHYVLTAI
                     AKDKISRSMIEIVR"
     gene            complement(3349..5625)
                     /locus_tag="PLE23_08435"
     CDS             complement(3349..5625)
                     /locus_tag="PLE23_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor B family protein"
                     /protein_id="HMX83328.1"
                     /translation="MKVTNIIIFLIFGICVANAQIAKKMELLSHWDPDTFPLVFAGQY
                     SDCWGITTPNGKEYAVIGSTPYIHFFDISQDGNPVEVAHFAGGNVSLWREFKSYKSRI
                     YAVADQGEEGLIIFDCSNAPTIVKTKQTTEFAKRTHMPFIDEPNGRLYLTGTNTQNGG
                     MIVLDLTKDPDNPTLLASVPLPGGYVHDVFVKDNIAYCSHGNNGLWVYDFTNPQNPVL
                     LGNMTNYPESGYNHSSWITKDNKYLVMADETHNTSVKVVDIQDLGDIKVIDLFKSALL
                     APANEASIAHNPYVRGNYSIISYYHDGVQVYDISNPLNVQNVAWYDTEPNNTDYAGFD
                     GLWGVYPFFESDKLVLSDLNHGLFVVKLTDIDLESIHSGEVPEPVTNHQGVVTICAGS
                     TVDLTVPPGAQFINWYKNGNMIQSSGNVLTVNETGVYKAVTWSHATVKESAEVKVLLS
                     QLQSPVLTTENGATGFCKGEVLKLCSNTTAPKVLWTINGNPLVNSGDCYPADQAGIYQ
                     QIAIDGLCEKASNELSIEEYPLPSTIISPEIAKFCPGDSVTLSATTDAVDWVWFKDDV
                     LIPGAKGNTYTAHLNGLYSAYIENEHCSTISDYAAVKVYDVIIPNILVAGDTLSVASG
                     SAFQWFYNGNKIEKANTSSIVADSSGVYYVHVTDENGCITASASINHIFTSGKEPYST
                     VFRLYPNPVSGNLSINLGGNTEYTEWTILTLDGKMLAGGKLYSTFENNIPVQSLTSGM
                     YLLQCKGESIRYMKFIKH"
     gene            5763..>6313
                     /locus_tag="PLE23_08440"
     CDS             5763..>6313
                     /locus_tag="PLE23_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX83329.1"
                     /translation="MDTLLDINNLSVSFNNGQSNVTAVKSISFQIYKGEIVCLVGESG
                     SGKSVAANSVMRLLDESKTMVIAERMSFMSKSGECLDILQLPKDKLPGLRGQEIGMIF
                     QEPMTSLNPVIKCGDQVAEGLLIHGLESTHSVKDKVLELFKEVGLDDSKRIFDSYPHQ
                     LSGGQKQRIMIAMAMACNPSLLIA"
BASE COUNT         2005 a         1198 c         1262 g         1848 t
ORIGIN      
        1 agttgtttgg tggagaaaag cgggacactg gcaaattctt tttttccgaa aatactgata
       61 tccttaaact ttacaccaag gtaggggaat gtactaatca gagtaagatc tatatcaccg
      121 actgtaacaa aagcatcgag atttttatta atatcctcca ttaccattgc tttaatctta
      181 tctttgtaga tgactggcaa tatgatggct gccgagagga gtattccgaa gatgactgat
      241 gcgataatca gtattttctt gagtgttttc ttcatgctgt tttatgtaat ttaaaccata
      301 acgtatttga agcaatatta gtttttgacg aagttttaaa gatttctaaa aatatgatga
      361 aacctgactc aataaatttt acttggatag atactgatca taaattaaag gatttttcaa
      421 aagaccatac tttacaacaa tggatagcat ttgataccga atttataggc gaacgccgcg
      481 acaaaacgtt gctctgcctt attcaggtga gctccgaact cggtattttt ctgatagatt
      541 gtataaaaat taatgatctg acaccttttt taaacctggt aatcaatccg gatatactca
      601 agatcacaca tgccggtgaa aatgattata aggttttgtt cgaattgtat cggataatac
      661 ctgttaatgt agcagatgtg cagatagctg ccggatttgc aggttacaaa tttcccgttt
      721 cgttagcagg attgatgtct ggcgaactga accgcaaact cgacaaatct tatactgtca
      781 ttgactggga acaacgcccg cttgatgaaa gatatattca gtatgccgtt gaagatgtgt
      841 tattccttaa acaaatatac gataggctac tagccagaat aacaaaagct ggtatggaat
      901 cctggataaa acaggaattc gaattcctga ctttaaggga aacttatgcc ggctcaccgg
      961 aaaaagaagc actggctgct atcaatctta cacaacttag tcaaaaagaa cgtattctga
     1021 tgctgcgact ataccaatgg cggtatgacc aagccgagct gaccggtaag tcaaaggaaa
     1081 aaatattacc aaaaaaatgg ataaacattc tggccaggca actaaagcac ggcaaaaagc
     1141 atctcctgag caataggtca atctcttcag gatttataga cgcacaatgg acacaactcg
     1201 aattactgct caatcaacca acaactgatg acgaaagaga actcgtcaaa aacgctaccg
     1261 gtgaaacgcc aaccgatccg gtgcatgaaa gcctgcagga tctggttatg agtatcatcc
     1321 gttaccagtg tcgccttaaa ggtgtcgatc ctgggctggt gatgccgcct catattataa
     1381 agttgcttaa aaggaaagtg acagatgctt ctaaaatact cgaaactacc tggagaagcg
     1441 atttgctggg tgatgaattt cttaaatgga tcgattttga aaatctacta agttttacat
     1501 ttgaagaaaa taagattatt atcgaaaaaa atgataaaaa taagaatcta aaacactgat
     1561 tgttaagtat aaataaatta ttgatatcac ttcattaaaa aaaattgatt tatgaggaaa
     1621 ttcattttgt gtgtattttc attttgttca atggctgtga taatacaggc acaaacgctt
     1681 aacgtagagt ggcaaaaatg cttgggagaa tcaggcaagg aagaacgccc gcttgggctt
     1741 accaggacag atcagagtgt aactgttatg tttggcacta catctaccac tggtgatttt
     1801 tccggcaata aaggtgaaac tgattggtat tacaccaagt ttgacagcaa acaagtaaag
     1861 aaatatggta aaatctatgg tggatcaaag atagaaacac tgtcatggat aaacgaaaat
     1921 tcagatcaga ctttcacagt aacaggtata accaattcta atgacggaaa tgttaataat
     1981 aaccatggtg gcaatgatgc ctgggcaata aaactgactg aaaatggcaa tattcaatgg
     2041 agaaaatgta taggcggcac aggtgatgac caaggttatt tttcggccga aacagctgat
     2101 aagaactacc ttttccttgg atcaacaact tcaaaagatg gagactttac caccaataac
     2161 ggtgggtctg atatttttat gataaaaaca gacatcaacg gtatctttca gtttactaaa
     2221 ttatatggcg gctccggaaa cgaaaaacct gtaggcatgg tcaaattaac ggatggttac
     2281 ataatacttg cagaatcgaa ttcgccatca atctcaggtg ctgccaatgc caacaaaggt
     2341 aaaactgact tcttgctcat aaaaacagac ttgaatggta atgcaagctg ggctaaattt
     2401 tttggcggac ctgatgatga caatcctgca cacatcactt tactcagtga cggtaatctc
     2461 cttgttactg gtactacttc ttccacttca ggtgatatta gtggcgcaca tggtggtaaa
     2521 gatatttggg tagctaaaat atcttctgaa gggacattgt tatgggcaaa aaaccttggt
     2581 ggttcaggta atgattccgg taaaatggct gtggaatatt cttataccaa tccggttaaa
     2641 tatttcatca tcggaagtac agcatccgcc aatggaggta acataaacgg tgctaaaggt
     2701 ggtagcgata tctgggttgc aaggttatct tctaccggaa attttgaaaa cggaaagaac
     2761 tttggtgggt caaaagatga ttttgcggga tcaataagtg tcatgaccga taattcactg
     2821 tacattacag gagaatccaa ctcaactgac ggcgatgtgg taggcaacaa aggcgggcaa
     2881 gatgtctgga tagccaaact ggcaaaaccg accgctacat tcgatattcc tcagagcttc
     2941 gacttaaagg tatttcctaa tcctgctgct acttctattc atgtaaaatg ggatgaccaa
     3001 actcctgtgg aattattgga tattacaagt ataaccggcg tgtcgatgat gcaaatccca
     3061 gtcgataaat cttccgggtt cactacaata gatttgggca acattccaaa tggtcactat
     3121 gtgctaacag cgatagctaa ggataaaatt tcaaggtcaa tgatagaaat tgttagataa
     3181 ataacttgac aatatgcaag aaatgatatc cgcttctatt ttgaaagaat cgccctgcat
     3241 ttaagctaat agttagatta acaaacagaa acggtgttac tattaaccag taacaccgtt
     3301 ttttatttaa atatgttgtg caattattgt tgcattagta tttgattatc aatgtttaat
     3361 gaacttcata taccggatgg attctccttt gcattgcagc aaatacattc cacttgtcaa
     3421 gctttgtaca ggaatgttat tttcaaatgt tgagtaaagc ttaccaccgg ctaacatttt
     3481 accatcaagc gtaaggattg tccattctgt gtattcagta tttccaccaa gattaatact
     3541 caaattacca gatacaggat tagggtaaag tctgaaaact gtagaatatg gttctttgcc
     3601 ggaagtgaaa atatggttga tactggcaga tgctgttatg cagccgtttt catctgttac
     3661 atgaacatag tacacacccg aactgtctgc tactatagaa ctagtatttg ctttctcaat
     3721 cttatttccg ttataaaacc actgaaaggc actacctgaa gcaacagata aagtgtctcc
     3781 ggcaacaaga atattcggaa taataacatc atataccttg actgcagcat aatcactaat
     3841 ggttgaacaa tgctcgtttt ctatataagc gctgtaaagt ccgttcagat gtgcagtata
     3901 tgtattccct ttagcacctg gaatcaaaac atcgtctttg aaccaaaccc aatcaactgc
     3961 atcagtcgtt gcacttagtg tgacagagtc acccggacaa aactttgcaa tttcaggtga
     4021 aattattgta gaaggaagag gatattcttc aattgatagt tcattagaag ctttctcgca
     4081 caagccatca atagcaatct gttggtaaat gccggcttgg tctgcggggt agcagtcacc
     4141 agaattgaca agtgggttgc cgttgattgt ccaaagcact ttgggtgctg tggtattact
     4201 acatagtttt aggacttcac ctttacaaaa tcctgttgca ccattctctg tcgtaagaac
     4261 cggactttga agttgtgaaa gcaatacctt cacctcagca gattctttta cagtcgcatg
     4321 gctccatgtc acagctttat atacacctgt ctcgttaacg gtcagcacat tacctgatga
     4381 ctgaatcatg tttccgttct tataccagtt gataaattgg gcacccggag gtacggtcaa
     4441 atcaactgta gatcctgcac atattgtgac aacaccttga tggttagtta caggctcagg
     4501 tacttcacca ctgtgaatgc tttccaagtc aatgtcagtc aattttacta caaacaatcc
     4561 atgatttagg tcagataata ccagtttgtc tgattcgaaa aatggataaa caccccaaag
     4621 tccatcaaac ccggcataat cggtattgtt cggctcggta tcataccatg ccacattctg
     4681 tacattcagc gggttgctta tatcatacac ttgtacaccg tcatgataat aagaaatgat
     4741 cgaataattg ccccgcacat aaggattatg cgctatgctg gcttcgttgg caggtgccag
     4801 aagtgctgat ttaaacaaat caatcacctt aatatcgcca aggtcctgaa tgtcaacaac
     4861 ttttacactt gtattatgtg tttcatcagc catgacaaga tatttattat ccttcgtgat
     4921 ccatgaactg tggttatacc ctgactcagg gtaattagtc atattgccaa gtaataccgg
     4981 gttttgcgga ttggtaaagt cataaaccca aagtccgtta ttgccgtgcg aacaataagc
     5041 tatattgtct ttgacgaata cgtcatgcac ataaccaccc ggtagtggta cactagccag
     5101 caatgttggg ttatccggat cttttgtcag atccaatacg atcattcctc cgttttgtgt
     5161 attggtaccg gtaaggtaga gcctgccgtt tggttcatcg atgaatggca tgtgtgtcct
     5221 tttggcaaat tcggtcgttt gctttgtttt tactatggta ggggcattac tacaatcgaa
     5281 tataatcaga ccttcctcgc cctggtcagc tacggcataa attctgcttt tataagactt
     5341 aaattcgcgc cacaaagaaa catttcctcc tgcaaaatgc gctacttcaa caggatttcc
     5401 gtcttggctg atgtcaaaaa aatgaatata tggcgtagag ccgataacgg catattcttt
     5461 accatttggt gttgttatac cccagcagtc tgaatattgc cccgcaaata ccaaggggaa
     5521 agtatcgggg tcccaatgcg ataacaattc catttttttg gcgatctgcg cattggccac
     5581 acaaattccg aaaatgagaa aaataataat gtttgtaact ttcattcaat ataagctttt
     5641 ttatgtgaaa ttaatatgtg tcataactaa cattgtagtt atgataacaa agataagttt
     5701 tatttataca tttgtaaata gcttctaatg tacaaatgta aatattactc gaattttgat
     5761 aaatggatac ccttctagac attaataatc taagcgtaag ctttaacaac ggacaaagta
     5821 atgtaacggc tgtaaaatcc atctcatttc aaatatacaa aggtgaaata gtctgcctgg
     5881 taggcgaatc gggctccgga aaatctgtag cagcaaactc ggtgatgcgt ttactggatg
     5941 aaagcaaaac catggtcatt gcagagagaa tgagttttat gtcaaaatca ggcgaatgcc
     6001 ttgatatcct tcaactacca aaagataaat tgccgggtct cagaggtcag gaaataggta
     6061 tgatcttcca ggaacctatg acttctctca atccggtcat caaatgtggc gaccaggtgg
     6121 ccgaaggtct gcttattcat gggcttgaat ctacacactc agtcaaggac aaggttctgg
     6181 agttatttaa ggaagtaggg ctggatgatt caaaaagaat atttgactcg tatcctcacc
     6241 agttatcagg tggacagaaa caacgtataa tgatagcaat ggcaatggct tgtaatcctt
     6301 cattacttat agc
//
LOCUS       DAOQQY010000164         6294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5305_length_6294_cov_5.731047, whole genome shotgun sequence.
ACCESSION   DAOQQY010000164 DAOQQY010000000
VERSION     DAOQQY010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5305_length_6294_cov_5.731047"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="PLE23_08445"
     CDS             complement(<1..241)
                     /locus_tag="PLE23_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83330.1"
                     /translation="MKKLILSIAVLFLLNNTLAFAIGRYEQYNNAIHWAYLSYADNLE
                     DCAENYTLFYGPCVDESNAILDADIRDAWDVFCICA"
     gene            complement(253..699)
                     /locus_tag="PLE23_08450"
     CDS             complement(253..699)
                     /locus_tag="PLE23_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83331.1"
                     /translation="MKKINFYLAMLILFLLVSCSKENQVVSESELYERWNVSEDVQDY
                     IYWARQNVENTLMLSQKFGVKVQDISKYRGKCDAHNNSISNETICDQIKKYENARNNL
                     VKSRVHLKQSNKELEKYRDSWVRYVASKMPSPGQLLSIKKEGQNNR"
     gene            complement(802..1251)
                     /locus_tag="PLE23_08455"
     CDS             complement(802..1251)
                     /locus_tag="PLE23_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83332.1"
                     /translation="MKKFIILLLLSMFVGFYANAKDSLIRPYVFSGNAKLNIVLDSLT
                     PDQKKEFLFKFIDKNYSLLKDSLSSNHKLNFPDHYLKLMMAQMGYSDSTVFKIDFDES
                     IHYFNNTMVNIQNPEQNPLPKLPCTDKFIVDVKICLGATVVAASVFG"
     gene            complement(1403..5713)
                     /gene="rpoC"
                     /locus_tag="PLE23_08460"
     CDS             complement(1403..5713)
                     /gene="rpoC"
                     /locus_tag="PLE23_08460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMX83333.1"
                     /translation="MTFRKKINKQSQAFDSITISLASPDDILERSFGEVLKPETINYR
                     SYKPERDGLFCERIFGPVKDYECYCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     KLVVPVVHIWFFKSLPNKIGYLLGLSSKKLESVVYYERYVVVQPGIKEKDGVNVYDLL
                     TEEEYLEILDKLPRENQMLDDEHPEKFVAKMGAEAVRDLLMKLDLDNLSYDLRNQAAN
                     ETSQQRKSEALKRLRIVEAFREGQTRMENKPEWTVIQYLPVVPPELRPLVPLDGGRFA
                     SSDLNDLYRRVIIRNNRLKRLLEIKAPEVILRNEKRMLQEAVDSLFDNSRKSNAVKAE
                     GGRALKSLSDILKGKQGRFRQNLLGKRVDYSGRSVIVVGPTLKLHECGIPKDMAAELF
                     KPFIIRKLIERGIVKTVKSAKKLVDRKDNVIWDILENILKGHPVLLNRAPTLHRLSIQ
                     SFQPRLIEGKAIQLHPLVCGAFNADFDGDQMAVHVPLSPTSILEAQMLMLSAHNMLNP
                     QDGSPVTLPSQDMVLGLYYLTKEKKSDENDVVKGTGKTFYSAEEVTIAFNEGKLDLHA
                     GIKVKARVEDEHGNFVQKLIETTTGRVLFNQVTPEQVPYINQLLTKRSLKQIISQILR
                     RTNFAVTAQFLDDIKELGFFWSFKGGLSFNLGDLINPSIKSKTLQDAQAEVDEVWDNY
                     NMGLITNNERYNQIVDKWTYADNRITDTLLKELSAHKRGFNSVYMMLDSGARGSKQQI
                     KQLCGLRGLMAKPRKSGDTGGQIIEDPILANFLDGLSVLEFFISTHGARKGLADTALK
                     TADAGYLTRRLVDVAQDVVIRESDCDTLRGIETMALKDGEKVIESLTSRIQGRFTLHD
                     IYHPDTEEVILNAGDYINDSMSELIEKAGVEMVTIRSVLTCETSHGVCAKCYGKNLAT
                     GRIAEMGDTVGIIAAQSIGEPGTQLTLRTFHVGGIASVSQTESETQAKYAGILEFDGV
                     RFTDINDDGVNQKVVLSRTGEIRIVEPKTGRVLSSSHIPYGSNLKVEDRKEIKKGEVI
                     CDWDPFNAVIISEFSGVASFEDVEEGVTYRIERDDQTGFAEKVIIESKNKKKIPSIKI
                     INADGEELRNYSLPVGAYISIEDGAEVFAGQKIVKIPRSLGKLQDITGGLPRVTELFE
                     ARNPSNPAVVSEIDGVVSFGKIKRGNRECSVTAKDGQTRKYLIPLSKHILVQEGDFVR
                     AGTPLSDGTIAPKDILSIKGSFAVQSYLVEGIQEVYRSQGININDKHIEVLVRQMMRR
                     VIIEDAGDTHFLEGEAVDKFEFAEQNEWIFDKKFITDPGDSTKLKAGQLVTLRQLREE
                     NSHLKRNDKKVVQFRDAISATSSPMLQGITRASLGTYSWISAASFQETTKVLSQAAIS
                     AKCDKLEGLKENVIVGKLIPAGTGVKTFIKDLYVASKDSYNATTTVKHELEKVGSEEA
                     "
     gene            complement(5769..>6294)
                     /gene="rpoB"
                     /locus_tag="PLE23_08465"
     CDS             complement(5769..>6294)
                     /gene="rpoB"
                     /locus_tag="PLE23_08465"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658379.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HMX83334.1"
                     /translation="FETVLGWAGQKLGVKFATPIFDGANTDEIEEYIQKANLPSLGQT
                     YLYDGETGDRFHQQATVGVIYMLKLSHMVDDKMHARSIGPYSLITQQPLGGKAQFGGQ
                     RFGEMEVWALEAFGAANILQELLTVKSDDIIGRAKTYEAIVKGDRLPTPNIPESFNVL
                     IHELRGLALDVRFE"
BASE COUNT         1755 a         1409 c         1107 g         2023 t
ORIGIN      
        1 gtgcacaaat acaaaaaaca tcccaagcat ctcttatatc ggcatccaga attgcatttg
       61 actcatcgac acatggcccg tagaataaag tgtaattctc tgcacagtcc tccaaattat
      121 ctgcataaga aagatatgcc caatggattg cattattata ttgttcatag cgcccaattg
      181 cgaacgctaa agtgttattc agcagaaata gtacagctat ggataaaatt aattttttca
      241 taacccaaaa atttaacgat tattttgtcc ttccttttta atgctcaata attgtccagg
      301 agaaggcatt ttagatgcca catatcttac ccaactatcc ctgtattttt ccaattcttt
      361 gttgctttgt ttcaaatgaa ctcgagattt tactaaatta ttccgtgcat tttcatattt
      421 ttttatttga tcgcaaatcg tttcatttga tatgctatta ttatgggcat cacacttacc
      481 gcggtatttt gaaatatctt gaacctttac accaaacttt tgcgacaaca tcaaagtatt
      541 ctccacattt tggcgtgccc aataaatata atcctgaaca tcttcagaca cattccaacg
      601 ttcgtataat tctgattcac tcacaacctg attttcctta gaacaggaaa ccagtagaaa
      661 cagaataagc attgccaaat aaaaattaat ctttttcatt tctaaattat ttatcattaa
      721 agactattta cttttcagac aagcatcaaa atcagctttc gcttgaacca aacctatgaa
      781 cgcctcaaac actccagcct cctaaccaaa aacactggca gccaccacag ttgcccccaa
      841 acaaatttta acatcaacta tgaatttatc tgtacatggt aattttggta atggattttg
      901 ttccggattt tggatattaa ccattgtatt attgaaataa tgtatgcttt cgtcaaaatc
      961 tattttaaaa accgtagaat ctgaatatcc catttgagcc atcatcaact tcaaataatg
     1021 atccggaaaa tttaatttat gattactcga taacgagtcc ttaagcaaac tataattctt
     1081 atcaataaac ttaaacaaaa attctttttt ctgatcaggc gttaatgaat caagaacaat
     1141 gtttaatttg gcatttccag agaagacata aggtcttatt aaactatctt tagcatttgc
     1201 atagaaacct acaaacatcg ataataataa aagaataata aactttttca taatacattt
     1261 ttcaattatt acaaaattaa gagattaaaa ttcaaattaa aattaaaatt ttaacatata
     1321 attaacatat agcaacataa aagacaaaag ccccgacaaa atatcattgt cgaggctttg
     1381 tattaaattt taattattat gcctaagctt cttcgctacc caccttttcg agttcgtgtt
     1441 ttactgtcgt ggtggcattg tagctgtctt tgctggcaac gtacagatcc ttgatgaaag
     1501 tcttaacacc ggtacctgcc ggtatcaatt ttccgacaat cacattttct ttcaagcctt
     1561 caagtttatc acacttggca gagatagccg cctggctcag tactttggta gtttcctgga
     1621 acgaagctgc tgatatccag ctgtaagtac ccaatgatgc tctggtaata ccttgaagca
     1681 tcgggcttga agtagcagat atggcatctc tgaactgaac aactttttta tcatttctct
     1741 tcaggtgtga attttcttca cgaagctgtc gcagcgttac tagctgtccg gccttaagtt
     1801 tggtcgaatc gcccggatcg gttataaact ttttatcgaa gatccattcg ttttgctctg
     1861 caaattcgaa tttatcaact gcctcacctt caaggaaatg tgtatcacct gcatcctcga
     1921 taattacacg tcgcatcatc tgtcgtacca atacttcgat gtgcttgtca ttgatattga
     1981 tgccctgtga gcgatatact tcctggattc cttcaacaag ataagactgt actgcgaagg
     2041 aacctttgat actaaggata tctttaggcg caatggttcc gtcagagagc ggtgtacctg
     2101 cccttacaaa gtcaccttcc tgtacgagga tgtgcttaga gagtggaatc agatatttgc
     2161 gagtctggcc atccttagcg gttaccgaac attccctgtt acctcgtttt attttgccaa
     2221 aagaaaccac gccatcgatc tctgatacaa ccgcaggatt cgaagggttt cttgcttcaa
     2281 agagttctgt tacccttggc agaccacctg tgatatcctg aagtttaccc aatgagcgag
     2341 ggattttgac gattttctgt ccggcgaata cttcggcgcc gtcttctatc gaaatgtatg
     2401 cccctaccgg gagtgaataa tttcttaatt cttcgccatc tgcattgatg atttttattg
     2461 acggaatctt tttcttattc ttagactcga tgattacctt ttctgcaaaa ccagtttggt
     2521 catcacgctc tatacggtaa gtaacacctt cttccacatc ttcaaaactt gctacacctg
     2581 agaattcgga aataatcaca gcattgaacg gatcccagtc gcaaatgact tcacctttct
     2641 taatctcttt tctatcttcc actttcagat ttgatccgta cggtatgtga ctagaagata
     2701 atactctacc ggtttttggt tcgacaattc tgatttctcc ggttcttgaa agtactactt
     2761 tctggttaac tccgtcatca ttaatatcgg taaacctgac tccatcaaat tctagaatac
     2821 cggcatattt tgcttgggtc tcagattctg tctgcgatac tgaagcgata ccacccacgt
     2881 ggaaagtacg aagggtaagc tgtgtacctg gttcgccaat agattgagca gcgatgatac
     2941 ctaccgtatc acccatttcc gcaattcgac cggtagcaag gttctttccg taacattttg
     3001 cgcaaacacc gtgtgatgtt tcacaagtaa gtacagatct tattgttacc atttctacac
     3061 ctgccttttc aatcaattca gacattgagt cattgatata atcacctgcg ttaagtatga
     3121 cttcttctgt atcaggatgg taaatgtcat gaagtgtaaa tcttccctga attctggaag
     3181 taagtgattc tataactttt tcaccgtctt tgagagccat ggtttcaata cctcttagtg
     3241 tgtcacaatc gctctcacga atgactacat cctgagccac atctacaaga cgtcgtgtaa
     3301 ggtatcctgc atctgcagtt ttgagtgcag tatccgcaag acccttacgt gcaccgtgtg
     3361 tagagataaa gaactcaagt accgaaagtc cgtcgaggaa gtttgccaag atcggatctt
     3421 cgataatctg accaccggta tcaccggatt ttcttggttt agccatcaat cctctcaatc
     3481 cacataactg tttaatctgt tgctttgaac cacgggcacc cgagtcaagc atcatataaa
     3541 cagagttaaa tcctcgtttg tgtgctgata attccttcaa caaagtatca gtaatccggt
     3601 tatctgcata ggtccattta tctacaatct ggttataacg ttcattgtta gtaataagac
     3661 ccatgttgta gttgtcccat acttcatcta cttctgcctg agcatcttgt agtgtttttg
     3721 acttaatgct cggatttatc aagtcaccga ggttaaatga cagaccaccc ttgaacgacc
     3781 agaagaaccc aagctccttg atatcatcta aaaactgtgc agtgacagca aaatttgttc
     3841 gtctgagaat ctgactgata atctgcttaa ggcttcgctt agttagcaac tgattgatat
     3901 aaggaacttg ctccggagta acctggttaa aaagcactcg accagttgtc gtctcgatta
     3961 atttttgtac aaaattgcca tgctcgtcct ccacccgggc ttttacttta atacctgcat
     4021 gtaggtcaag ttttccttca ttgaaggcaa ttgttacctc ttctgcggaa tagaatgttt
     4081 tgcctgtacc tttgactaca tcgttctcat cagatttttt ctcctttgta aggtaataca
     4141 gaccgagtac catgtcctgt gaaggaagcg tcaccggcga accatcctgt gggttaagca
     4201 tattatgtgc agacaacata agcatctgag cctcaagaat agaggtaggg cttaatggca
     4261 cgtgaacagc catctggtca ccgtcgaagt cagcgttgaa ggcaccacac accaatgggt
     4321 gtaactggat ggccttccct tctatcaatc ttggttggaa tgactgaatg gataatctgt
     4381 ggagtgtcgg tgcccggtta agcaatacag ggtgaccttt cagaatattt tcaaggatat
     4441 cccatattac attatctttt ctgtcaacaa gttttttagc tgattttacg gttttcacta
     4501 taccgcgctc tatcagtttt ctgataatga acggtttgaa cagttcggca gccatatcct
     4561 taggaatacc acactcatga agttttaggg taggccctac cacaatcact gaacggcctg
     4621 agtagtcaac ccttttaccg agcaagtttt gtctgaaacg tccttgtttt cctttgagta
     4681 tgtcgcttaa tgattttagt gcccgaccac cttcagcttt tacagcgtta gatttgcgtg
     4741 agttatcaaa aagtgagtcc accgcttcct gcaacattct tttctcgttt cgcaaaatta
     4801 cttccggagc ttttatctca agtagtcttt tcagacggtt gtttctgatg ataactcttc
     4861 tgtacaaatc attcaggtcg gagctggcaa aacgacctcc atcaagaggc accaaaggtc
     4921 ttagctcggg cggcaccaca ggcagatact ggataacagt ccattcgggt ttgttttcca
     4981 ttctggtctg tccttcacgg aatgcttcca caatacggag acgcttgagc gcttcactct
     5041 ttctttgctg cgaagtttca ttggcagcct ggttgcgtaa atcgtatgac aaattgtcga
     5101 gatccagttt cattaacaga tcacgaacag cttctgcacc catctttgca acgaactttt
     5161 ccggatgttc atcatcaagc atttgattct cacgcggaag tttatccaat atttcaagat
     5221 attcttcttc tgtgagcaaa tcatagacat tcacgccatc tttttcttta atacccggct
     5281 gaacaaccac ataacgctcg taataaacaa cactttcaag cttcttggat gagagaccca
     5341 aaaggtaccc gattttattc ggaagtgatt taaagaacca tatatgtaca accggcacaa
     5401 ccagtttgat gtgtcccatc ctttcacgcc ttaccttctt ttcggtcact tctacaccac
     5461 atctgtcaca tactattcct ttgtatctta ttcttttata ctttccacag taacactcat
     5521 aatccttaac cgggccgaat attctttcac agaacaatcc gtctctttcc ggtttgtagc
     5581 tacgataatt gatcgtttca ggctttagta cttcaccaaa tgatctctca agaatatcat
     5641 cgggcgaggc taagctgata gtaatactat caaatgcctg actttgttta ttgatttttt
     5701 ttctgaatgt cattctttta ttattaaaat aagattgaat tatttttatc ctttcttttg
     5761 ttcagatatt attcaaatct gacgtcaagt gcaagacctc tcaattcgtg aatcaataca
     5821 ttgaatgatt ccggaatatt aggagtaggc agtctatcac ctttgacgat tgcttcgtac
     5881 gtcttggcac ggccaatgat atcatccgat ttgacagtaa gcaactcttg aagaatgttg
     5941 gcagcaccaa atgcttctag tgcccatact tccatctctc caaaacgctg tccaccaaac
     6001 tgtgccttac cacccaacgg ttgctgagta ataagcgagt aagggccgat agaacgtgcg
     6061 tgcatcttgt catccaccat gtgtgatagt ttgagcatgt agataacacc tactgttgcc
     6121 tgttggtgga atctatcacc tgtttcacca tcatacagat aagtctgacc caaggaagga
     6181 agattcgctt tctggatgta ttcttctatt tcatcggtgt tggcaccgtc aaagatagga
     6241 gtagcgaatt tgacgcccaa tttttggccg gcccaaccta acacggtttc gaat
//
LOCUS       DAOQQY010000165         6291 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5306_length_6291_cov_5.603111, whole genome shotgun sequence.
ACCESSION   DAOQQY010000165 DAOQQY010000000
VERSION     DAOQQY010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5306_length_6291_cov_5.603111"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PLE23_08470"
     CDS             <1..1345
                     /locus_tag="PLE23_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMX83335.1"
                     /translation="SMDKVPFTHLLKEIDEALLHLQNPVTSKKKVAVFFGGYSFERHI
                     SVESGRNIFEKLASSTKYEPIPIFVTGNNDHHDLYQVPINLLLKDNADDIRDKILHFS
                     KHPVVEKIKQQCKDITVKYASSSMVFEPVKVEYQELANKLDGVFIALHGRPGEDGTVQ
                     AILDQYGIPFNGSSSSSSAVTINKYNTLQLLRKNGFTVANQLLVTKSDFEKNHEQTLH
                     LIEDSFDYPFIAKPNDDGCSSAVKIIRNREQLLNYCVGVFRDEILLDTELRKRLKLGD
                     KEEFPQKDNILFESLITADGATHFLEITGGLLTHIKDGTLLYEMFEPSEALAGGEVLS
                     LEEKFLAGEGQNITPARFSKDKQAFEKISAKVKSDLQRAAEILNIRGYARIDAFVRIY
                     SPDQVETIIIEVNSLPGMTPATCIFHQSAINGYKPYEFIDQILEFAFNNKIPTHA"
     gene            1338..2183
                     /locus_tag="PLE23_08475"
     CDS             1338..2183
                     /locus_tag="PLE23_08475"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HMX83336.1"
                     /translation="MPNNFRIFMKDVGGFLLSKYFYIAALKIAGLILLLGGGFFMLLS
                     NFTDHGNSKILPNFIDKHIADVENTADNEGFNIKIVDSVHMVGKTGGLVLTQLPKPGS
                     RVKTSRTVYVTVSKYRPDQVKLASLPLLYGREFANTQKYLKQSFFIDSEVIGYQFDEG
                     PENHILAAIYKNDTIDNERTRKNDVFIEKGSKIQFILSKATDDMVRVPNLVCNTLDQA
                     RFLLSSSMLSEGSIIPDQTVTNQQSAYVYKQSPAHDSGKTLKKGDLVNIYLTQELPDN
                     CKDEN"
     gene            2266..4743
                     /locus_tag="PLE23_08480"
     CDS             2266..4743
                     /locus_tag="PLE23_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83337.1"
                     /translation="MPKIFTFLLLLFALCSNAQVVELPLESNNLLTGKQPITRKTSWY
                     TSTPGQRDATFNCVLPGGEFSLELDTAGLGNGHTVTVLKNPSTGTVQITDQHLLVFKA
                     SATTDFALDTVLVKLCNADGSKCNEFDYYFATERASANFTQPTVSVGENIKSYYTIPL
                     NQTPSAVVSLQLSGNAEGTKAVGFIESPDQFVFYSGRGGYNGQIKIKACDAYCVCDEY
                     IYNIKVTADTLDLPFVDDFSYPGPYPDPALWINDDVFINNTFGVKAPSLGVATFDGLN
                     ERGRPYQGGHGTSDYLTSRQLNLGKYNLNSNVYLSYYVQPQGNGNAPESIDSLLLEFK
                     DKDGNWVLLQGLVPGFQLTKDTFKFFSISITNEKYLYDGFQFRFVNQSDNTGMLDNWN
                     VDYISITAGIIPSLNTSDIAFSLPPKGLLKNYTAIPWQQFRNFEAQELNMNGNTLNLS
                     IDMYNFFNKTETADPSSLKISEKNTGTVLVNNLTLLELPPLAPEDQRNLTPGFHHFDA
                     TKDLPGFVDQLKNNFGTGNDFRFITEYKIDNSSEINLPVIKRNNTVLHETNMSYYYAY
                     DDGTAESNIGVKKNGSRCAVKFHANVNDTLRAIQLYIPRVFNNVSNQLFNLLVWKGSL
                     NSEPIHSDFLLKPVYLDNLFDTIQGFTTYVLMNEELSGPEPAFIPAGDFYVGWQQATD
                     DEFPIPIGFDKNNPDAAKNTFVNLEAGWQSLESLNFNGAVMIRPVLGNTKLNHTPDLV
                     GTKEQHNAGYEIFPNPASDRLFIYNKNQQATIEKSEIYNMQGMLKKKIDDRTNLSISD
                     LENGMYIIKITDKQSKLSSTIKFIVSH"
     gene            4758..5060
                     /locus_tag="PLE23_08485"
     CDS             4758..5060
                     /locus_tag="PLE23_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX83338.1"
                     /translation="MDITVSELKSKIESGERFIFLDVREPAEYNEFNIGATLRPLGMI
                     EQTAQEFADHKNDEIVVHCRSGARSGAAKDYLTAQGFTNVRNVLGGILAWQDSFGS"
     gene            complement(5070..5606)
                     /gene="dcd"
                     /locus_tag="PLE23_08490"
     CDS             complement(5070..5606)
                     /gene="dcd"
                     /locus_tag="PLE23_08490"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMX83339.1"
                     /translation="MILTDTKILQSIENGEIVIEPFDRSCLGTNSYDVHLGKYLAVYQ
                     DRILDARKHNKITELEIGPEGFVLYPGTLYLGVTQEYTETHNSVPFLEGKSSVGRLGI
                     DIHATAGKGDVGFCNHWTLEISCAQPVRVYEGMPIGQLIYFGVDGLIENFYNKKPGAK
                     YNDRSIRPVESMMWKNKF"
     gene            complement(5644..>6291)
                     /locus_tag="PLE23_08495"
     CDS             complement(5644..>6291)
                     /locus_tag="PLE23_08495"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83340.1"
                     /translation="TGNVVKKYAHQGEIFALNVVGEYLYSIGKDGWLVKWDLHRMDAL
                     QSIQLSYTSLRSFVHSEKSGKAYIGDSNGIVHILDLDTMASLLNFQAHDSTVFSLELN
                     EQNKLISGGKDAHLRLWDTDKFTHPLVDHPAHWFSVYAIRRHPTLPVIATASRDKQIR
                     IWNADNIEPILTLGYEKYQGHIRSVNNLLWTDNGRYLVSAGDDAVIIFWEFDYFQ"
BASE COUNT         2003 a         1336 c         1250 g         1702 t
ORIGIN      
        1 gtcaatggac aaggtaccat tcacccattt actcaaagag atagatgaag ccctgctaca
       61 tttgcaaaat ccggtgacaa gcaagaagaa ggttgctgtt ttctttggtg ggtattcatt
      121 tgagcggcat atttcggtag aaagcggtag aaatattttc gaaaaactcg ccagtagcac
      181 caaatacgaa cccattccga tattcgtcac cggcaataat gatcaccatg atctctacca
      241 ggtgcctatc aaccttttgc tgaaagacaa tgctgatgat attcgggaca aaatacttca
      301 tttcagcaag catccggttg tcgaaaaaat caaacaacaa tgcaaagata tcaccgttaa
      361 atatgcttca tcttcgatgg tattcgagcc ggttaaagta gaataccagg agttggcaaa
      421 caagcttgac ggcgtattca tagcacttca tggcaggccg ggtgaagacg gcacagtaca
      481 ggcaatactc gaccaatatg gcataccgtt caatgggtca tcaagcagtt cgtctgcagt
      541 tacaatcaat aaatacaata cactccagct gttgaggaaa aatgggttta cggtagccaa
      601 ccagttgttg gtgacaaagt cagattttga aaaaaaccac gagcaaaccc ttcatttgat
      661 agaagattca ttcgattatc ctttcattgc caaacctaac gacgatggtt gtagctcagc
      721 tgtaaagatt atccgcaacc gcgaacaatt gctcaactat tgcgtgggag tatttcggga
      781 tgaaatactg cttgatacag aacttcgcaa gagattaaaa ctcggtgaca aagaagaatt
      841 tccgcaaaaa gacaatattt tgttcgagtc gctcataacg gctgatggtg ctacacattt
      901 cttagaaata acaggtgggc tacttacaca catcaaagac ggaacattgt tgtacgaaat
      961 gttcgagcca tcagaagcgc ttgcaggtgg agaagtatta tcacttgaag aaaaatttct
     1021 ggcaggagaa ggacaaaata taacacccgc aagatttagc aaagacaagc aagcgttcga
     1081 gaagatttca gcaaaagtaa aatcagactt acaacgggca gctgaaatcc tgaacataag
     1141 agggtatgcc agaattgatg catttgtgag gatttattca cctgaccagg tggagaccat
     1201 catcattgag gtaaattcct tacctggcat gacacctgct acctgtatat ttcatcaaag
     1261 tgccatcaat ggttacaaac catacgagtt tatagatcaa atcctagaat ttgccttcaa
     1321 caacaaaata ccaacacatg cctaataatt ttagaatatt catgaaagat gtgggtggct
     1381 ttttactaag caaatatttt tacattgctg cactgaaaat cgcaggccta atacttttgt
     1441 taggtggcgg cttttttatg ttgttgtcca attttacaga ccatggcaat agtaaaattt
     1501 taccgaattt cattgacaaa catatagctg atgtagaaaa tactgcagat aatgaagggt
     1561 ttaacataaa aatcgtcgat tctgtacaca tggtaggcaa aaccggtggt cttgtactta
     1621 ctcagcttcc aaaaccaggt tcacgagtaa aaaccagcag gacagtctat gtaacggtct
     1681 caaaatatag gccagaccag gtaaagcttg catctctgcc actactgtac ggacgtgaat
     1741 ttgccaatac acaaaaatat cttaaacaaa gcttttttat tgattcggaa gtcataggtt
     1801 atcagtttga cgaaggcccg gaaaatcaca tccttgccgc catctacaaa aacgacacca
     1861 tcgacaatga gcgaacacgc aaaaatgatg tttttataga aaaaggatcg aaaatacagt
     1921 tcatcctatc aaaagcgact gacgacatgg tacgagttcc gaatctggtg tgtaatacac
     1981 ttgatcaggc cagatttttg cttagttcgt ccatgctatc agaagggagc atcatcccag
     2041 accaaacagt aaccaaccaa cagtccgcct acgtctataa acaatcgccg gcgcatgatt
     2101 cgggcaaaac attgaagaaa ggtgacttgg ttaatatcta cctgacacag gaattgccgg
     2161 ataactgcaa ggatgaaaac tgataagaaa gcacaatact aacaacataa tattgatttt
     2221 ttcgttgaaa tactgaaatt ttcaggtcac atcaattttt acaaaatgcc taaaatattt
     2281 acatttctgt tgttgttatt tgctttatgc tcaaatgcac aggtagtgga attaccgctt
     2341 gagtctaata atttgctgac aggaaagcaa ccgataacca gaaagacaag ttggtacaca
     2401 agcacaccgg ggcaaaggga tgcaactttc aattgtgtac tacccggtgg cgaattcagt
     2461 cttgaactgg atacggcagg cttaggtaac ggccacactg taacggtatt aaaaaatcca
     2521 tctacaggca ccgtacagat taccgaccaa catttacttg tatttaaggc atcagcaaca
     2581 accgattttg cacttgatac agttttggta aaattatgca acgcagatgg aagcaaatgc
     2641 aacgaattcg actattattt cgctacagaa cgtgcttcgg ccaacttcac tcagccaacg
     2701 gtttcggtag gcgaaaatat caaatcatac tatacaatac ctctcaacca gacaccatct
     2761 gctgtggtat ccttacaact atcaggtaat gccgaaggta caaaagcagt cggttttatt
     2821 gagtctcccg atcagtttgt attctattcc ggccgtggcg gttataacgg gcagattaaa
     2881 ataaaggcct gcgatgcgta ttgtgtttgt gatgaataca tatacaacat aaaggtaact
     2941 gccgacacgc tcgacctacc atttgtagat gacttttcgt acccgggacc ttatcccgac
     3001 ccggcattat ggattaatga tgatgtgttc atcaataata ctttcggtgt caaggctcca
     3061 tctttaggcg ttgctacttt cgacggccta aatgaaagag gacgtccata tcagggaggt
     3121 catggtacgt ctgactattt gacatccagg caacttaatc ttggcaaata caatcttaac
     3181 agcaacgtat atctgagtta ttatgttcag cctcagggca atggcaatgc gcccgaatcg
     3241 attgattctc tcttacttga gtttaaagac aaggacggca actgggtact tttgcaaggt
     3301 ttggttccgg gatttcagct cacgaaagac acgttcaagt ttttctctat ttcgattacg
     3361 aatgaaaaat acctgtatga tgggttccag ttcaggtttg taaaccagtc ggacaataca
     3421 ggcatgcttg ataactggaa tgttgattat atttcaataa cggcaggcat tattccttca
     3481 ctcaatacat cagatattgc attttcgctg ccaccaaaag gactattgaa aaattatacg
     3541 gctataccat ggcaacaatt caggaacttt gaggctcagg agctcaacat gaatggcaat
     3601 acactcaacc taagtatcga catgtataat tttttcaata aaacagagac agccgaccca
     3661 tcttccttaa aaataagcga aaaaaataca ggaacagtat tggttaataa ccttaccctg
     3721 cttgaattgc caccattggc acccgaagac caaaggaacc taacaccggg attccaccat
     3781 tttgatgcga caaaagattt acccgggttt gtcgatcagt taaaaaacaa tttcggcaca
     3841 ggcaatgatt tccgatttat tacagaatac aaaatagaca attcatcaga aattaactta
     3901 cctgtcatta aacgcaacaa cactgtactc cacgaaacca acatgagcta ttattatgcc
     3961 tacgatgacg gtactgccga gtcgaatatc ggcgtaaaga aaaacggtag tcgttgtgct
     4021 gtaaaatttc atgccaatgt taatgatacg ctccgcgcaa tacaactcta cataccaaga
     4081 gtgtttaaca atgtatcgaa ccaactcttc aatcttttgg tttggaaagg aagtctcaat
     4141 tctgagccaa tccattctga tttcctgtta aaaccggttt atctcgacaa tcttttcgat
     4201 actattcaag gttttaccac ttatgtactg atgaacgaag aactgagcgg accagaacct
     4261 gcattcattc cggcaggaga cttttatgta ggatggcagc aggctaccga tgatgaattt
     4321 cctatcccga taggttttga caagaataat cctgatgcag ccaagaatac atttgtaaat
     4381 cttgaggcag gctggcagtc gctcgaatca ctcaatttca atggtgctgt catgataaga
     4441 ccagtacttg gcaacaccaa actcaaccac acaccagacc ttgtgggtac taaagaacag
     4501 cacaacgccg gctacgaaat atttcccaat ccggcatctg atagattatt catttataac
     4561 aagaatcagc aggcgaccat tgaaaaatcg gaaatataca atatgcaggg aatgttgaaa
     4621 aagaagattg acgaccgtac caatctcagc atttcggatc ttgaaaacgg catgtacatc
     4681 attaagatta ccgataagca gagcaaactc agctctacca tcaaatttat tgtctcacat
     4741 taattcaacc aaacaaaatg gatataacag taagcgagct aaagtcaaaa atcgaatcag
     4801 gcgaaagatt catcttctta gatgttcgcg agccggcaga gtacaatgaa tttaacattg
     4861 gagctacatt acgaccgctt ggaatgatcg aacagacggc acaagaattt gcggatcata
     4921 aaaatgacga gattgtagtg cattgccgtt cgggtgcccg cagtggtgct gctaaagatt
     4981 atcttacagc acaaggcttt accaatgtca ggaatgtgct tggcggtatt ctggcatggc
     5041 aagatagttt tggcagttaa tttatctgct cagaatttat ttttccacat catagattct
     5101 acagggcgta ttgacctatc gttgtatttg gctcctggtt ttttgttgta aaaattttca
     5161 atcagtccat caacgccaaa gtaaatcagc tgtccgatgg gcattccttc ataaaccctt
     5221 acaggttgag cacaggaaat ttcgagtgtc caatgattgc aaaagccaac atcaccttta
     5281 cctgctgtag catgtatatc tataccaagc cttcctaccg atgatttacc ctctagaaaa
     5341 ggaacagaat tatgtgtttc ggtgtattct tgtgtgacgc ctagatacag cgtgcccggg
     5401 tagagcacaa aaccttccgg ccctatctcc agttctgtaa ttttgttgtg cttgcgggca
     5461 tcaagtatcc tgtcttgata tacggcgagg tatttgccaa ggtgaacgtc ataagaattg
     5521 gtaccaaggc atgaacggtc gaatggctcg atgacgattt cgccattttc aatagattgt
     5581 aggattttcg tatctgtaag tatcatagat gagtgtcgat tgggcgcaaa gatactattt
     5641 cgtctattgg aaataatcaa attcccaaaa aatgatgaca gcatcatcac ctgccgacac
     5701 aagatacctt ccattatcag tccacaacag attgttaacg ctccgtatgt gtccctgata
     5761 tttttcataa ccgagtgtca ggataggttc gatattgtcc gcattccaaa tcctgatttg
     5821 tttatcgcgc gatgccgtag caatgaccgg caaagtagga tgccgccgaa tggcataaac
     5881 cgaaaaccaa tgtgcaggat gatcgaccag aggatgagtg aacttatcgg tatcccacaa
     5941 tctcaggtgg gcatcttttc caccacttat gagcttattc tgctcattga gttcaagaga
     6001 aaaaacggta gaatcatgtg cctgaaagtt aagcaggctc gccatcgtat caagatctag
     6061 tatatgcact atcccattac tgtcaccaat ataagcctta ccactctttt cggaatgaac
     6121 aaacgacctt agcgaagtgt aacttaactg aatgctctgt aaggcatcca tcctgtgaag
     6181 atcccatttc accaaccagc catcttttcc gatagaataa aggtactcac ctactacatt
     6241 caaagcaaaa atttcacctt gatgggcata cttttttact acattacctg t
//
LOCUS       DAOQQY010000166         6287 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5310_length_6287_cov_5.080712, whole genome shotgun sequence.
ACCESSION   DAOQQY010000166 DAOQQY010000000
VERSION     DAOQQY010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5310_length_6287_cov_5.080712"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PLE23_08500"
     CDS             <1..880
                     /locus_tag="PLE23_08500"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMX83341.1"
                     /translation="EANGITAVAFINTFVATAWGVSYVGATPVFADCDEYYNIDTQQL
                     RKLITSKTKAIIGVHLYGQPCDIDALLAICREHDIYFVEDAAQAHGATYKGTRVGSFG
                     EMACFSFYPGKNLGAYGEGGGVTTNNESYRNKMFSLRSHGSQVRYYHDEIGYNMRMDG
                     IQAAVLDIKLQHLAQWNNKRNQIAEKYLQQINNPLVKLPLIRKDVVSAFHLFVVTVPD
                     RGRFMQYMTENGVATAQHYPVPCHLQKAYSFLQYKKGDFPKSEYLAEHCVSLPMFAEL
                     TDNEVHKIINLVNNFK"
     gene            complement(951..1103)
                     /locus_tag="PLE23_08505"
     CDS             complement(951..1103)
                     /locus_tag="PLE23_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83342.1"
                     /translation="MSNILHFTSHISPLTTYHSPKLREGQKRARSKADGLTRQGHAQY
                     YTNQTI"
     gene            complement(1336..2421)
                     /locus_tag="PLE23_08510"
     CDS             complement(1336..2421)
                     /locus_tag="PLE23_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HMX83343.1"
                     /translation="MSQFDKNIPYNDLPLLPPNADIETKKILRKTILAGRALAQLNGT
                     LKNLPNPTLFLDTIYLQEAKASSEVENIITTNDELYKSLVADRKVENSATKEVLSYKE
                     ALWLGFEQLKKKPFITTNLCINIVQCIKQNTASIRVTPGTTLNNTKGEVIYTPPSGEN
                     VIREKLANLEMFINEDVTIDPLIKMALMHYQFEAIHPFADGNGRTGRILLLLYLKLSG
                     LLDTPAIFLSEYIIKNKADYYKSLRSVTEKNDWENYILYMLDMIEETSARGSERLGKI
                     TTAIDKTAEEIKTKLPKIYSKDLVEILFRLPYTKRQHLIDENIGNPKTVGNYLIALEE
                     NGFLKSVKVGKEKLYLNERLLKILEGE"
     gene            complement(2650..2874)
                     /locus_tag="PLE23_08515"
     CDS             complement(2650..2874)
                     /locus_tag="PLE23_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83344.1"
                     /translation="MKGIAWFFLYKEFKDQKFEEEITKLMLDDDSKRGIYTYVLTRKE
                     RFLNIRAFTDNQQRECYERQKGICPVGTEH"
     gene            complement(2920..3258)
                     /locus_tag="PLE23_08520"
     CDS             complement(2920..3258)
                     /locus_tag="PLE23_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83345.1"
                     /translation="MVNCQSSKKQLNGSGMPPSFKVGFETINIAGKELTKQALRNAVF
                     SGSWVSDANRYFSENTRPKIDDEYLSGLTNSQEYLETVIEWISSGNIKRYISDRQYVQ
                     AAIEFWNYFS"
     gene            complement(3589..4512)
                     /locus_tag="PLE23_08525"
     CDS             complement(3589..4512)
                     /locus_tag="PLE23_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMX83346.1"
                     /translation="MEILSVGIIITVLLLFFSVVIIQQGTVGVITFFGKYSRIMRPGL
                     NFKIPVVETIYRRISIQNRAVELDFQAITHDQANVYFKVMLVYAVVDQQEDTIVTVAF
                     KFMDERSLMQALTRTIEGTIRAFVATKKQAEILGLRTEIVTHVKEEVDETLLGWGYHL
                     LDLQLNDITFDDAILKSMAQVVASNNLKAAAENEGQALLITKTKAAEADGNAIRISAE
                     AERTAAQLRGQGVALFRKEVTDGMASAVKELKDNDLDSSLVAFSMWTETIKHMAEYGK
                     GNVIFLDASNESLEKTMKQMLAVTVKDLTKE"
     gene            4735..5436
                     /locus_tag="PLE23_08530"
     CDS             4735..5436
                     /locus_tag="PLE23_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMX83347.1"
                     /translation="MNKGALRLYKKLKHKAFQPKSICEVGVFNPEESNILLFIKENVK
                     TTLIEADPEYASNIRKYFGDLPTLTVIEAAVYDYNGTVELYKRASSTFIATLESSPAL
                     VNDNYKKDKKDSFLAKSILFSDIDTGEFELVSIDIEGAEWFVLKHMNSRPAVISIETH
                     GKYYTNPHYDKIKKWMSENKYELWFTDSSDSVFIRRGTFKVNIFEKLKLLANELKFWL
                     VRRKKIFKNLTRKNK"
     gene            complement(5417..>6287)
                     /locus_tag="PLE23_08535"
     CDS             complement(5417..>6287)
                     /locus_tag="PLE23_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83348.1"
                     /translation="PMLIEIQGFPSVFFFQIIFDRLYRNVYQLPVELSPYFTGMGPEA
                     FTELLRKVIMDGNKPEEVIILELDPLHQATYIDFAGAEKALGIKTICVRDIIHEGKCL
                     YYMNNGQKQHIKRIFNRVIADDWAKRPDLKAEFRFTEDIEVSWAGHPNWFFRLSKYSM
                     PYLKSDYVPETRILSEIKEMPADLENYVLKPLFSFSGSGVIFNVTKEDIERVDTPTDY
                     ILQKKVVYHPGIVSLDEPVKCEIRVMLVWPDDAEKPYFTTNLTRLSKGDLIGVKFNKN
                     KSWVGASLTYFSE"
BASE COUNT         1837 a         1299 c         1132 g         2019 t
ORIGIN      
        1 tgaagcgaac ggcattacag cagtcgcttt catcaacacc tttgtcgcca ccgcatgggg
       61 cgtgtcgtat gtaggcgcta caccagtgtt tgccgactgc gacgaatact acaatattga
      121 tacacaacag cttcgcaagc tgataacatc caaaacaaaa gccatcatcg gtgtgcatct
      181 ttatggccaa ccctgtgata tcgatgcact tctggctatt tgcagggagc atgacattta
      241 ctttgtagaa gatgcggcac aggctcatgg tgctacttat aaaggcacac gagtaggcag
      301 ctttggcgag atggcatgct ttagcttcta tccgggcaag aaccttggtg cctacggcga
      361 aggtggcggc gtcactacca acaacgaatc ataccgcaac aagatgttca gcctgcgcag
      421 ccacggcagc caggtacgct actaccacga cgaaataggc tacaacatgc gaatggacgg
      481 catccaggcc gctgtacttg acataaagct ccagcacctg gcacaatgga acaacaaacg
      541 aaatcagata gctgaaaaat acctgcaaca gatcaacaat ccactcgtca aactaccatt
      601 gatacgcaaa gatgtcgtgt cggcttttca cctgttcgtc gttaccgtac ccgacagggg
      661 cagatttatg cagtacatga ccgagaatgg cgttgctact gcacaacatt atccggtgcc
      721 gtgtcatttg cagaaagcat acagttttct acaatacaag aagggcgact tccctaagtc
      781 agaatatctg gccgaacatt gtgtctcctt accaatgttt gcagaactaa ctgacaatga
      841 agtccataag ataataaatt tagtcaataa ctttaaataa acaattcagc agttttttca
      901 aaaacaagct acaacaactc tcatccttac aacacatcgc tacaagttag ctatattgtt
      961 tggttggtat agtattgggc gtgcccctgt cgggtcaggc catccgcttt acttcgtgcc
     1021 cgcttctgtc cttcacgcaa ctttggtgaa tggtaagtgg taagtggtga aatgtgagat
     1081 gtgaaatgta aaatgttaga cattgtaaca catttttttg cggcattttt tgaaccatta
     1141 agcagaagat tttttaaccc ggataacgca ttaggcaaaa tgatagaatc atattttccg
     1201 gaaagttatt agtctaataa agagttcgta cgcaaggcag ttaatgttag ccatttgaat
     1261 aatttcaaat ctgtctaaat gaagttctga aaaacagcaa gattgtttat ggatttgaat
     1321 tttgtaagta tcgttttatt ccccctctaa aattttcaac aatcgttcat tcagatacaa
     1381 tttttccttt cctactttta cagatttcaa aaaaccgttt tcttctaacg ctattaaata
     1441 atttccgacg gtttttgggt ttccgatgtt ttcatcaatc aaatgttggc gtttggtgta
     1501 tggcaaacga aacaagattt caaccaaatc tttggaatat atctttggta atttggtttt
     1561 tatttcttct gctgttttgt ctattgctgt agttatttta cctaaacgct ccgaacctct
     1621 tgccgaagtt tcttcaatca tatccaacat atacaaaatg tagttttccc aatcattttt
     1681 ttcggttaca ctacgcaggc ttttgtaata atcagctttg ttttttataa tgtattcact
     1741 caaaaaaatg gctggtgtgt ccaataatcc tgaaagttta agatatagca ataacaaaat
     1801 ccgtcctgtt cttccgttac cgtctgcaaa tgggtgaatt gcttcaaatt gatagtgcat
     1861 taacgccatt tttatcaatg ggtcaatcgt tacatcttca ttgatgaaca tttccaaatt
     1921 tgccaacttc tcacgaatga cattttcgcc acttggtggc gtataaatga cttcgccttt
     1981 tgtgttgttc aaagttgttc ctggtgtaac ccgaattgaa gcggtgtttt gtttaataca
     2041 ttgcacaatg ttaatgcaaa gattggttgt aataaatggc tttttcttta attgctcgaa
     2101 acctaaccaa agtgcttctt tataacttaa tacttctttg gtagctgaat tttcaacttt
     2161 tctgtcggca accaacgatt tgtaaagctc atcatttgtt gtgatgatgt tttcgacttc
     2221 cgaactcgct tttgcttctt gtaaataaat ggtgtctaaa aacaaagttg ggtttggtaa
     2281 atttttcaaa gttccgttta gttgagctaa agctctacct gccaaaattg ttttacgcaa
     2341 tatctttttt gtttctatat ctgcgtttgg tggcaataaa ggcaggtcgt tgtacggtat
     2401 gtttttatca aattgactca tttcgttcaa gtgtaaattt tacgctcgct tctaatacaa
     2461 gggcaaaaat actaattttt tactctcgtt gtatcaacag aggtaaaaat tacgctcgtt
     2521 tgtttttttt gcttattttc ccgattttct tctgttatca tctttgcata acatttggca
     2581 attttcggca gtggtttttc ctcctaaatg ccaaggtgta atttggtcgc cttccatttc
     2641 gttgatttct taatgttcgg taccaacagg acaaattcct ttttgtcttt cgtagcattc
     2701 acgttgctga ttgtctgtaa atgcccgaat atttaaaaat ctttcttttc gtgtcaaaac
     2761 gtaggtgtaa atacctctct tgctatcatc atcaagcatt agttttgtaa tttcttcttc
     2821 aaatttttgg tctttaaatt ctttgtacaa aaaaaaccaa gcaatgcctt tcatttctct
     2881 tctgaatttt ggaaaagttg cttttgacca attgattacc taactaaaat aattccaaaa
     2941 ttctattgct gcctggacgt attggcggtc tgatatataa cgcttgatgt tgccactcga
     3001 aatccattct attactgttt ctaaatattc ttgactgttt gtcaaaccgc ttaaatattc
     3061 atcgtcaatt ttaggtctgg tgttttcaga aaaataacgg tttgcgtcag aaacccacga
     3121 acccgaaaaa acagcatttc gcaatgcttg ttttgttaat tcttttcctg caatatttat
     3181 tgtttcaaaa ccaactttga aggatggtgg cattccgctc ccattaagtt gttttttact
     3241 actttgacaa ttaaccatct gcctctaaca ctgtttgaag tgtttgagta tgcagtccta
     3301 agactgtacc aaaaaatatg gcttaccaaa agcccaaata caagtctctt tattcgcgat
     3361 tcctatagcc tatacaaagc aaaaacacca acgctcatat atttcgattt attaacccgg
     3421 agaacgtatt aagcatttaa acgaaaatga tgagtacaaa aaatccataa aaaagatata
     3481 aaagcatagt gaactatggt ttttaaactt ttaaaaatgg atttgaagta atcattattt
     3541 gcagtaaaaa gcctaatgct ttgtcttggt ttaatgaaca actctcagtt actctttcgt
     3601 cagatctttt accgtcaccg caagcatttg cttcatcgtt ttttcaagcg attcatttga
     3661 agcatccaag aaaatgacat tacctttacc gtattctgcc atgtgcttga tggtttcagt
     3721 ccacatagag aatgccacca gtgaactgtc gagatcattg tctttgagtt cttttacagc
     3781 cgatgccata ccgtcggtta cttcctttct gaataatgct acaccctgcc ctctcaactg
     3841 agccgctgtt ctttcagctt cagcagatat ccggatggca tttccgtcag cttctgcggc
     3901 ttttgttttg gtaatgagca atgcctggcc ttcgttttcg gctgctgcct ttagattatt
     3961 ggaggccact acctgcgcca ttgacttcag gatagcatca tcaaatgtaa tatcatttaa
     4021 ttgcagatca agtagatggt agccccatcc aagtagcgtt tcatccactt cctcctttac
     4081 gtgcgtaaca atttcggtac gtaagcctaa tatttctgct tgtttcttag tagcaacaaa
     4141 tgccctgatg gtgccttcga tggtacgagt aagtgcctgc atcaggcttc tttcatccat
     4201 gaatttgaac gcaactgtaa cgatggtatc ttcctgctga tccactacgg catatactag
     4261 cattacttta aagtaaacat ttgcctgatc gtgcgttatt gcctgaaaat caagctctac
     4321 ggccctattc tgtatggata ttcgacgata aatagtctct accaccggaa ttttaaaatt
     4381 aagccccggc ctcatgattc tcgaatattt cccgaaaaat gtgataacac caaccgtacc
     4441 ctgttgaatg atgaccacag aaaagaacaa taatagcact gtgatgatga tcccaactga
     4501 taaaatttcc ataattatac actttattta gaccacaaga taaccataaa agtgttgcta
     4561 agctaagttt tttcgatttt ttttgaatat atcattgttg tattaactct gtataacaat
     4621 gtgtaatata ttgcatccta aagaatgcag cgggcatatt tagcataaaa gtcaagtaag
     4681 cccaatgaac tcggaatcat caaaattttc ttactgaagt aaattattca tgaaatgaac
     4741 aaaggtgccc ttcgtttata taagaaatta aaacacaaag cttttcagcc aaagagcatt
     4801 tgtgaggtag gtgttttcaa cccagaggaa tcgaatatcc tcctatttat taaagaaaat
     4861 gtcaaaacga cactcataga agctgaccct gagtatgcat ccaacatacg gaaatatttt
     4921 ggcgatctgc ccacgctaac tgttatagag gctgctgtat atgattacaa cggcactgtc
     4981 gaactctaca aacgtgcatc atctacattc atcgctacac tggaatcaag tccggccctg
     5041 gtaaacgaca attacaaaaa ggataaaaaa gacagttttt tggcaaaatc tatattattc
     5101 tcagatattg acaccggcga atttgagctt gtgagcatcg atatcgaagg ggccgaatgg
     5161 ttcgtactca aacacatgaa cagcaggcca gccgttattt ctatagagac acacggcaaa
     5221 tactatacga atccgcatta cgacaaaatt aaaaaatgga tgtcagagaa taagtacgaa
     5281 ttatggttta ctgacagcag cgatagtgtt tttatcaggc gaggaacctt taaagtgaat
     5341 atatttgaaa aattaaaact actcgcaaac gaattaaaat tctggttggt gcgacgtaaa
     5401 aaaatattca aaaatcttac tcggaaaaat aagtaaggct cgctcctacc cagcttttgt
     5461 tcttattgaa ttttacgcca ataagatccc ctttgctcag tcttgtaagg ttggtagtga
     5521 agtatggttt ctctgcatca tcgggccaca caagcattac tcttatctcg catttgaccg
     5581 gttcatccaa cgaaacgata cccggatgat agactacctt tttctgaaga atataatcgg
     5641 taggtgtatc aacacgctcg atatcttctt tagttacatt gaagatgacg ccgcttcctg
     5701 agaatgaaaa caatggtttg agcacataat tttccaaatc agcaggcatt tcctttattt
     5761 ctgacaaaat ccttgtctca ggcacgtaat ctgacttcag gtaaggcatc gaatatttcg
     5821 acaggcgaaa gaaccagttg gggtgccctg cccagcttac ttcaatgtct tccgtgaagc
     5881 gaaattctgc cttcaggtcc ggacgctttg cccaatcatc agctattacc cggttaaata
     5941 tccgtttgat gtgttgcttt tgaccgttat tcatataata aaggcactta ccttcatgga
     6001 taatatccct aacacagatt gttttgatgc caagggcctt ttcagcacct gcaaaatcaa
     6061 tgtacgtagc ctgatggagc ggatcgagtt cgagaatgat gacttcttcc ggtttatttc
     6121 catccattat caccttcctc aacaattcgg taaaagcttc cggccccata ccggtaaaat
     6181 aaggagacaa ttcaacaggt agttgataaa catttctata taatctgtca aatattatct
     6241 gaaagaaaaa tacacttggg aagccttgta tctctatgag cattggt
//
LOCUS       DAOQQY010000167         6253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5335_length_6253_cov_5.571636, whole genome shotgun sequence.
ACCESSION   DAOQQY010000167 DAOQQY010000000
VERSION     DAOQQY010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5335_length_6253_cov_5.571636"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..878
                     /locus_tag="PLE23_08540"
     CDS             207..878
                     /locus_tag="PLE23_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83349.1"
                     /translation="MEKNVTTRYSDQDLAEFEVVIREKLENARKELEFNREQIIELHE
                     NSSNQQGGDYFDDSSLHTELEMLNQMVSRQRQFVQNLENALIRIKNKTYGICIATGEL
                     IDKKRLLLVPHATKSVVAKINETAKIEPVSSPSFDSYAMFDRDQELEKETPARVSKTV
                     TKIVKKSSASKKTTPQNEDDFDFLDNEDLGPVNDDDFEYEDDVLNVDIDDIPDDSMDM
                     SDIDN"
     gene            911..1114
                     /locus_tag="PLE23_08545"
     CDS             911..1114
                     /locus_tag="PLE23_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83350.1"
                     /translation="MHKSFDKNSKNLKPTKKKAGLNNITKRLSSKEADDDQYVNLVAK
                     PKKKVPKKFFMDDEDEISYRVKH"
     gene            complement(1202..4501)
                     /locus_tag="PLE23_08550"
     CDS             complement(1202..4501)
                     /locus_tag="PLE23_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HMX83351.1"
                     /translation="MKKLNCALLIVFTLLNLTVSAQKKSNEKKAVKETVKDEFLEPGT
                     YSGLSFRSIGPAVTSGRIADFAVVPSHPEIYYIASASGGVWKTTNKGTTFEPIFDSEG
                     SYSIGCVTLDPSNSNVVWVGTGENHNQRSVAYGDGVYKSEDGGKSWTNMGLKNSEHIS
                     NIIVDPTDPRIIYVGAYGPVWKEGGERGVYKSTDGGTSWSLVKSVSQYTGCNNLIMDP
                     RDPKVLYAAFHQRMRKVFTYIGGGPESALYKSTDGGVTWKKLEGGLPGGDVGRIGIAI
                     APSNPDVLYAVVEAKDGKGGIYRSSDRGASWTKQSGVFTSGNYYQEVDVDPKNENRIF
                     ITDTYNKVSDDGGKTVRNLGEINKHVDNHAIWIDPNNTDHLLVGCDGGIYETYDFAKT
                     WEFKSNLPVTQFYKVSTDNAVPFYNVHGGTQDNFSLGGPSRNISANGIVNSDWIVTSV
                     GDGFETQVDPTNPDIIYAQSQYGGLVRFDRKSGQYLNLKPSEKKGEAPLRWNWDAPLL
                     ISSHDPSRLYFGANVLFRTDNKGQEWRAISPDLSRQSDRNKFEVMGMVQSVDAIAKNQ
                     STDIYGQLTTIAESRIDPDILYTGTDDGLIQITTDGGKTWTKIDNIPGAPTQSYVHQI
                     ITSWHDKNTAYACFNHHRYGDFKPYIFKTVDGGKSWKSISANLPERGSVYSIAEDHVD
                     AGLLFVGTEFGVFFSKDGGSNWIQLKAGLPTIAVRDIEIQRRENDLVLATFGRGFYIL
                     DDYSPLRNFKRDDFNKSAKILPVKDAWMYIESTPLGLRDKGHLGSGYFSAQNPPVGAV
                     FSYYLKESIKTIKEKRQETEKTKKAKGETFYYPSMDSLYAEDNQLSPYLRFTIKDASG
                     NIVRYLTAPAKKGLARIVWDFRYGTPAPSNQRYTPAPDQLFSAEELGHLALPGTYTVT
                     LEKFEDGNFSVLDGPVNFVCKSLNNASLPATDLKEYSDFCTKVSKLRRAVSAGDAILS
                     ELKDKILNFDIAVKDMPTENKALIRKISDIKQELTKLDYEMNGDATLARREFESSPSI
                     NGRVSDIEGSMWSVTSAPTETFKTTYTIAAEDFGKWLEKLKKADQAIRQAELELNAGN
                     APATPGRWPQW"
     gene            complement(4635..5681)
                     /locus_tag="PLE23_08555"
     CDS             complement(4635..5681)
                     /locus_tag="PLE23_08555"
                     /inference="COORDINATES: protein motif:HMM:NF016159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HMX83352.1"
                     /translation="MTDNNLNILLNLSLHHIGNKVNGGELILSDNELDIAAPDFNHAV
                     RSFFLESFEWLEYYNFTFSNDEISLNPVYNFCERIFRDQTAFHSLSQEIAGYLYEHTT
                     HPQIKNGDLLVALFENVLLEDELVDVIGIFKSENKQGFLKVDVTGHSFSVDLIEGIQL
                     DKLDKACLVFNTEKESGYRIALLDRSNKITEAQYWREQFLNVKPAEDAYHQTRDFLQV
                     TKNFITRELPKETGIDKSEQAELLNRSIGYFKSNTNFNKEEFEETVFQDPVVRESFRH
                     FEPGNNKPSMHEFADSFEISDNAVRKQSKVYKSVIKLDKNFHIYVHGDRELIEQGRDP
                     DGRKYYKIYFNEET"
     gene            complement(5808..>6253)
                     /locus_tag="PLE23_08560"
     CDS             complement(5808..>6253)
                     /locus_tag="PLE23_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83353.1"
                     /translation="FGYLRKRNAEPGTPGKIGWGLFISACSTLVMVIATYYCHNGAVK
                     GSAWWLIGTYGVVTVGELFLSPMGLSLVSKLSPPRITALMMGGWMLAISIGNKLSGVL
                     ATMWDKYEVKANFFWVNFVLLMIAVVILLSMMKRLNRIFNQYSGG"
BASE COUNT         1740 a         1330 c         1247 g         1936 t
ORIGIN      
        1 acaagtgctg tcagaggaat aaattattca gaaattattg tttaataatt ttttgcacga
       61 tatggcttta tattataatt ttgcacatta ttcaaaaaaa aactttcaaa atattgttaa
      121 ataaattatc atttctatat taacactata atatttttaa ttaatcaatc ccttaccaca
      181 tactttcaat ttaactaaat tttttaatgg aaaagaacgt aacaacaagg tacagtgatc
      241 aggatttggc cgaattcgaa gtggtcattc gtgaaaaact ggaaaatgcc agaaaagaac
      301 tggagtttaa ccgcgagcag attatcgaac tgcatgaaaa cagcagcaat cagcaaggtg
      361 gtgactactt tgatgacagc agcttgcaca ccgagctcga aatgttgaat caaatggtct
      421 caagacaaag acagtttgtt cagaatcttg aaaatgctct aatacgtatt aaaaataaaa
      481 cttacggtat ctgtattgcc accggcgaac ttattgacaa aaagcgtctg ctactggtac
      541 cacatgctac taagagtgtt gtagcaaaaa ttaacgaaac agctaagatc gaaccggttt
      601 cgtcaccatc ctttgactcg tacgctatgt ttgaccgcga tcaggaactc gaaaaagaaa
      661 cacctgcccg tgtaagcaaa accgttacta aaatagtcaa aaaatcttcg gcaagtaaaa
      721 agacaactcc acaaaatgaa gatgattttg acttccttga taatgaagat ctcggtccgg
      781 ttaacgatga cgacttcgaa tacgaagacg atgtattgaa tgttgacatc gacgacattc
      841 cagatgattc aatggatatg agtgatattg ataactaaat aaaaatagaa ttttaattgc
      901 gtttgttgcc atgcataagt catttgataa aaattcaaaa aacttaaaac cgacaaagaa
      961 aaaagcggga ttaaataata taacaaaaag gctaagtagt aaagaggccg atgatgacca
     1021 atatgttaat ttggttgcaa agcccaaaaa gaaggtgccc aaaaaattct tcatggatga
     1081 tgaagatgaa ataagctaca gggtcaagca ttaattctac ttctattaaa taacattctg
     1141 ccggcgtgtt tcaatacaaa catccggcag aatacttctt ttgcccaaac ttatagctgc
     1201 cctaccattg tggccaccta cccggtgtag ctggcgcatt accggcattc agttcgagtt
     1261 cggcctgacg gattgcctgg tcagcctttt ttagtttttc cagccatttg ccaaaatctt
     1321 ctgctgcaat ggtgtaagtt gttttgaaag tctctgttgg tgctgaagtt acagaccaca
     1381 tacttccctc aatgtcagat acacggccat tgatggaagg cgaggattca aactctcttc
     1441 tggcaagcgt ggcatcaccg ttcatttcgt aatcaagttt tgtcagctcc tgtttaatgt
     1501 cagatatctt gcgtattaat gccttgttct ctgttggcat atcttttacg gcaatgtcaa
     1561 aattaagaat cttgtctttt agctcgctca atattgcatc tccggcgctt acagctctgc
     1621 gcagtttcga tacctttgta caaaaatcag aatattcttt cagatcggtg gctggcaaac
     1681 ttgcattgtt taacgatttg cagacgaaat tgacaggacc gtcaagtact gaaaaattac
     1741 cgtcttcaaa tttttcaagt gtgaccgtat aagtaccagg taatgcaagg tgtccaagtt
     1801 cttcagctga aaataattgg tctggtgccg gagtatagcg ctgattggaa ggggcaggtg
     1861 ttccgtacct gaagtcccac actatgcggg ctaggccttt cttagccggc gcagtcagat
     1921 atctcacaat attacctgaa gcatccttaa tcgtaaaacg caagtaagga ctgagctggt
     1981 tgtcttcggc atacagcgaa tccattgatg gatagtaaaa tgtttctcct ttagcttttt
     2041 tggttttttc tgtttcttga cgtttttctt ttatggtttt aatgctctct ttcagataat
     2101 agctaaatac agctccaacc ggtggattct gtgccgaaaa atagccacta ccaaggtgac
     2161 ctttatcgcg taggccgagt ggagtgcttt ctatgtacat ccaggcatct tttaccggaa
     2221 gtatttttgc acttttattg aagtcgtctc tcttaaaatt gcgcaacggt gaataatcgt
     2281 caagtatata aaatccacga ccgaatgttg caagtaccag gtcattttct ctgcgctgta
     2341 tttcaatatc gcgaactgct attgttggta gccctgcctt taattgtatc caattacttc
     2401 cgccatcttt gctgaaaaaa acaccaaact ctgtacctac aaagagcaaa ccggcatcta
     2461 cgtggtcttc tgctatactg tacacactgc ctcgttctgg taagttggca ctgatagatt
     2521 tccagctctt gcctccgtca acagttttaa atatgtaggg tttaaagtct ccgtatcgat
     2581 gatgattgaa gcaggcataa gccgtattct tgtcatgcca cgaggtgatg atttgatgaa
     2641 cataagactg tgtcggtgca ccaggtatgt tgtcaatttt tgtccaggtc ttgcccccgt
     2701 ctgtggttat ctgtataagc ccgtcatcag tgccagtata taaaatgtca gggtctatcc
     2761 tcgactcggc aatggtagtc agctggccat atatatctgt actttggttt ttggctatgg
     2821 catctacact ttgtaccata cccatcactt caaatttgtt tctgtctgat tgtctgctta
     2881 ggtcaggact tatggctctc cattcctgcc ctttgttgtc ggtgcggaac aatacattgg
     2941 caccaaaata caatcttgat gggtcatggc tgctgatgag caaaggtgcg tcccaattcc
     3001 agcgaagtgg tgcttcgcct tttttctcgc ttggtttcaa gttgaggtat tggccacttt
     3061 ttctatcgaa gcgaaccaag cctccatact ggctttgagc ataaatgata tcagggttgg
     3121 tggggtccac ttgtgtttcg aatccgtcac ctacagatgt cactatccag tcagagttta
     3181 ctataccgtt tgccgaaata ttcctcgacg gtccaccaag gctaaaatta tcttgtgtgc
     3241 caccatgcac attataaaac ggcacagcat tatctgtaga tactttgtaa aattgtgtca
     3301 ccggaagatt tgatttaaat tcccaggttt tggcaaagtc ataagtttca taaataccgc
     3361 cgtcacaacc tacgagaagg tgatctgtat tgttggggtc tatccagatg gcgtggttgt
     3421 ccacatgttt gtttatttcg cccagattcc tcactgtttt gccaccatca tcacttactt
     3481 tgttgtaagt atccgtaata aagattctgt tttcattttt tgggtcaaca tctacttcct
     3541 ggtaataatt gcctgaagtg aatacaccgc tttgtttggt ccagctggca ccacggtcag
     3601 acgaacgata aataccacct tttccatctt tggcttcaac tactgcgtag agtacatctg
     3661 ggtttgaagg ggcaatggca ataccaattc ttcctacatc ccctccggga agaccacctt
     3721 cgagcttctt ccatgtaaca ccaccatccg ttgatttgta caatgcagat tctggtccgc
     3781 cacctatgta agtaaatact tttctcatcc tttggtgaaa tgcggcatat agtactttcg
     3841 ggtcgcgcgg gtccatgatc agattattgc agccagtgta ctggctaaca gattttacaa
     3901 gactccaact ggtaccacca tcggttgact tgtacacgcc tctttcacca ccttccttcc
     3961 atacaggacc ataggctcct acatagatga ttcttgggtc agtagggtca actataatgt
     4021 tcgaaatgtg ttctgaattt tttaatccca tattcgtcca gcttttaccg ccgtcttcac
     4081 ttttatagac accatctccg taggctaccg aacgttggtt gtggttctcg ccggtaccca
     4141 cccacacaac atttgagttg cttgggtcta atgttacaca gccaatagaa tacgatcctt
     4201 cactatcaaa tattggttcg aaagtagttc ctttgttggt tgttttccat accccgcctg
     4261 aggcagaggc tatgtaataa atctccgggt gagatggaac tactgcaaaa tcagctatac
     4321 gacccgatgt gacagccggc ccgatgcttc tgaatgacag accgctgtac gtgcccggtt
     4381 ctagaaattc atcttttact gtttctttta cggccttctt ttcatttgat tttttttgtg
     4441 cagataccgt taagttcaac aaagtaaaaa ctatcagtaa ggcacaattc agttttttca
     4501 tatcataata tttttaataa taaaccacta aattataaat ggaaatgaaa tttttagcat
     4561 tgctcaagcc gaaaatcgcc taaagcgcac aaaacattga caaaaggatg agccgatctg
     4621 cacaacaatg tatatcaggt ctcttcatta aaatagatct tgtagtattt tctgccgtcc
     4681 gggtcacggc cttgttctat cagctcacgg tcgccatgta cgtaaatgtg gaaattttta
     4741 tcgagtttaa taaccgattt gtacactttt gattgcttcc tgacagcatt atccgaaatc
     4801 tcgaacgaat cagcgaattc gtgcatcgat ggcttgttat tgcccggctc aaaatgcctg
     4861 aatgattcgc gtacaaccgg gtcctgaaat acggtttctt caaattcttc tttattgaaa
     4921 ttggtattgg acttgaaata tccaattgac cggttgagta attcggcttg ttcggacttg
     4981 tcaatacccg tttcctttgg cagttcgcgg gtgatgaaat tttttgtaac ctgtaggaaa
     5041 tctctcgttt ggtgataggc atcttcagct ggttttacat tcagaaactg ctcgcgccag
     5101 tattgtgcct ctgttatttt gttagaacgg tcaagtagtg caatacgata accggattct
     5161 ttctctgtat tgaacaccag acatgccttg tcgagtttgt cgagttgtat gccttcaatc
     5221 agatcgacac tgaacgaatg acctgtaaca tctactttca ggaaaccctg cttattttcg
     5281 gatttgaaaa ttcctatcac atctaccagt tcatcttcga gcagtacgtt ttcgaagagt
     5341 gccaccaaga ggtcgccgtt ttttatttgc ggatgtgttg tatgctcgta aaggtatccg
     5401 gcaatctcct gcgacaacga gtggaatgca gtttgatcgc ggaatatgcg ttcgcaaaaa
     5461 ttatagaccg ggttaagact tatttcatca ttcgaaaagg tgaaattgta atattcaagc
     5521 cattcaaatg attccagaaa aaaactccgc actgcatggt taaaatccgg tgcagctata
     5581 tcaagttcat tatcggagag gatgagttcg ccaccattga ctttattgcc aatgtgatgc
     5641 agagatagat ttaagaggat attcaggtta ttgtcggtca ttgtgtggtg gtttgagtat
     5701 tcaaagttac tcaattattt ctttctattt aagagtgtct ttcagtataa agcttattga
     5761 tttcagaaaa ttgaaatgaa aagtaccgga ctttatgtaa tgtaaaatta tccaccggaa
     5821 tactggttaa atatacggtt gagccttttc atcatagaca atagaataac tacggctatc
     5881 ataagcagga caaaatttac ccagaaaaaa ttggctttca cttcgtattt atcccacatg
     5941 gtggcgagca cacctgacag tttattgcct atagagatgg ccaacatcca gccgcccatc
     6001 atcagagctg ttattcttgg cgggctgagt ttgctcacca gtgacagacc catcgggctc
     6061 aggaataatt cgccgactgt gactacgcca taagtgccta tcagccacca ggcgcttcct
     6121 ttcactgcac cattatggca ataataagta gcgattacca ttacaagggt cgagcaggca
     6181 gaaatgaaca gtccccagcc tatttttccg ggtgtacctg gttcggcatt ccttttcctc
     6241 aagtagccga aga
//
LOCUS       DAOQQY010000168         6247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5341_length_6247_cov_4.663921, whole genome shotgun sequence.
ACCESSION   DAOQQY010000168 DAOQQY010000000
VERSION     DAOQQY010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5341_length_6247_cov_4.663921"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="PLE23_08565"
     CDS             <1..820
                     /locus_tag="PLE23_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HMX83354.1"
                     /translation="FEPESSVALGFGFRCGFLGMLHLEIIQERLSREFDQEVITTVPN
                     VSYHAYLTKDPTTKVVIHTPNDLPEPTVLDRVEEPYIIAQIITAPEYIGSIITLCLEK
                     RGHIKKQSYLTPERVEMVFELPLAEIVFDFYDRLKSISRGYASFDYSPLDYRESDLVK
                     MDIRLNSEPVDALSALVHKDKAQALGRQICSKLKELLPRQQFMIAIQASVGAKIIARE
                     TISAMRKDVTAKCYGGDISRKRKLLEKQKKGKKKMRQIGSVEVPQKAFLDVLKL"
     gene            complement(891..3320)
                     /locus_tag="PLE23_08570"
     CDS             complement(891..3320)
                     /locus_tag="PLE23_08570"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83355.1"
                     /translation="MKSGLFTFLVTITSIFYIQVCAFAQCNDPFEPGLNCETAPVFCF
                     TQDLDGYCTKLPDFSNPTAPIPMCSGSGGGVPNNSLWLGFVAASNNMDLLLLADNCTS
                     AAGSFGFQAGIYKGNCGGPFEAIACQGLCRTDSINLSTKSLIPGELYYIVLDGCNGSV
                     CDITIELKNGELAPAVLDDPIGPIASKSNEICIGDSVLFSIPPVKGASAYYWTLDEKL
                     VGAPNLMSNSVKLNFTTPGSHQLCVDVANSCFDVSLPPTQQCIDISVYNLTLADADVT
                     SLLCFGDSAGTISITPQGGLAPYSFNWSTGATTPLLDNLKAGNYTVTVSDALSCNKIF
                     SFEINQPDSLQIVVTNTNMTNNNINNGKISVVASGGTAPYTYLWNIGATTTTIDSLGE
                     GIYTVTVTDSKGCTLVQNIFMSGPDCGLSITFAKEHNNCNGGSEGTACVQVAGNFGPY
                     IYKWNGTPGDSCLTNLKAGYYFVTIVNTAGCEVKQFVEIKQPPPITVPDSSITIITES
                     YFGAGDAQVVLSPTNGTAPYSFLFDNGTTDGTLLAAGNHIVTITDANGCTSEFHFNVP
                     AIPCDSLGVQFVDIDIVKTCNNDTVSVSIINISGGKEPYSILWSNDSTSISVKDLYEN
                     ITALTVTDSFHCSLVIPLNIVKPAELQVTLDFTHTSQGKSNGKVTAIIKGGEAPFKCL
                     WSTGDTLATIKNLSPGTYCVTIIDAQGCMLVKCGTVEDGTSTNELLSAGWKLFPNPAN
                     DFIFLKQIHPDASPISISIYDVEGKELLSTIKQNTSGVIQVDISHLPPGSYYLSLKHK
                     DRDLYAKFLVK"
     gene            complement(3487..5004)
                     /locus_tag="PLE23_08575"
     CDS             complement(3487..5004)
                     /locus_tag="PLE23_08575"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83356.1"
                     /translation="MKTMLQTIAAIFILVSIQSQALFGQCNKPFKPGNSCETAPVICS
                     VGDLDGYCTTLQDVVNPSGPNPLCLSNGGGVPNNTAWIGFVAGSSNMAIEVIPGNCLD
                     VGGNTGFQTGIITGCPPNSKEIVCTGTCVNAPYLLSSQNFVPGKLYYLWIDGCGGPFC
                     DETIDMIQGSIQSSDPPGSIGNINGENHLTCTSSFVTYSVPPVKGATSYYWTLDGLSV
                     GDIKKDTNSISLQIKDAGTHLLCVDVANYCHDVTSLPAQKCFQIEVEGILLTDTTLVP
                     LLCYGDKNGAVGFEVSGGTPPYSFQWSTGDTTSSISNLKAGKYLLTVKDDSVCSKNVV
                     FNLSQPDSLELILYIFNTSGPDLLDGKSILNVVGGTPPYKILWSTGDSVNVLDKLSPG
                     GYCVTVTDFNDCVKYECGVVEKGSGTGTNEPLCSDWKLFPVPAKDYLYLQHLQPDNRA
                     VQLRIVSAEGKEMVRRHEKNTTSTIPLFIGNLPSGVYYLAVFHDSKQLYARFLKQ"
     gene            complement(5343..>6247)
                     /locus_tag="PLE23_08580"
     CDS             complement(5343..>6247)
                     /locus_tag="PLE23_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83357.1"
                     /translation="FGANDASVVVSPSGGVPPYQVFYDNGASDGTMLQAGNHTVEVKD
                     SLGCSVIFPFFVPAYPCDSLGIKAIDLDVQTSCFDKPVTLSIKNISGGQPAYNILWSN
                     GSTDSTVTDTFKNLTSLKVTDAKNCLFTLILDIDQPDSIFVNFEINDATPGNTDGSVL
                     VSATGGQGTFSYLWQDGSTTELLTGLAPGNYCVTITDAAGCSVVKCAEVKLKVSTNDL
                     ISQGWKLFPNPAKDFLYLKQAIVDGSQVSWQILDASGTNILTGEQKNTAGTIPVSVAH
                     LPSGQYYLLLHHHSKQLYAKFIIK"
BASE COUNT         1862 a         1319 c         1270 g         1796 t
ORIGIN      
        1 attcgaaccc gaatcttctg ttgcattggg atttggattc agatgtggtt ttctcggtat
       61 gcttcacctc gagattatac aggaaaggct ttcacgcgaa tttgaccagg aagttattac
      121 caccgtaccc aatgtgtcgt accatgcata cctgaccaaa gatcctacaa caaaggttgt
      181 cattcataca cccaacgacc tacccgaacc aactgtgctt gacagagtag aagagccgta
      241 catcatagcg cagattatca ctgccccgga atacattggt tctatcataa cactttgtct
      301 tgaaaaacgc ggccacatca aaaagcaatc gtacctaaca ccagaacgtg tagaaatggt
      361 attcgaactg ccactggccg aaattgtgtt tgatttttat gacagattaa aatccatttc
      421 acgtggatat gcttcgtttg actactcgcc gcttgattat cgcgagtcgg acttggtaaa
      481 aatggacata agacttaaca gcgagcccgt ggatgcatta tcggcactgg ttcacaaaga
      541 taaagcacag gcattgggtc ggcaaatttg ttcaaaactt aaagaattat tgccaagaca
      601 acagtttatg attgccatcc aggcttcagt aggtgcaaaa atcattgcaa gggaaaccat
      661 ctctgccatg aggaaggacg taacggctaa gtgctatggt ggtgacatct cgcgtaagcg
      721 taaactcctt gaaaagcaga aaaaaggcaa gaagaaaatg cgccaaatcg gctcggtaga
      781 agtaccgcaa aaggcattcc ttgatgtgtt gaaattgtaa aagttgcttg ttaaatcata
      841 aaaaaaatcc cgcctgttta ttcaagcggg attttttaga tatacgaaaa tcatttcact
      901 aaaaacttcg catacagatc tctgtctttg tgtttcagtg acaaataata acttcccggg
      961 ggcaggtgac tgatatctac ctgaatgaca cctgaagtat tctgcttgat tgtactgagc
     1021 aattccttac cttcaacatc atatatactg atagatatag gtgaagcatc ggggtgaatc
     1081 tgtttcaaaa aaataaaatc attcgcagga ttggggaata atttccagcc agcactgagc
     1141 agttcgttag tggaagtgcc gtcttctact gtgccacatt ttacaagcat acaaccctgt
     1201 gcatcaataa tggttacaca gtaagttccc gggctaaggt tcttgatagt agcaagggtg
     1261 tctcctgtac tccataggca tttgaatggt gcctcgccac ctttgattat tgccgtcact
     1321 ttcccattag acttgccctg tgatgtatga gtaaaatcga gcgtcacttg taactcagca
     1381 ggtttaacaa tgttgagtgg aataactagg ctacaatgga atgaatctgt tacggtcaat
     1441 gcagttatat tttcatacaa atctttgaca gatatagatg tactgtcatt actccaaagt
     1501 atggagtaag gttcttttcc accagaaata ttgataattg aaacagaaac tgtatcgttg
     1561 ttgcacgtct tgactatgtc aatatctaca aactgtactc ccaacgaatc acatggaatt
     1621 gccggtacat taaagtgaaa ttctgacgta catccgttgg catcagttat ggtcacaata
     1681 tgattgccgg cagcgagcaa agtaccatct gtagtaccat tgtcgaaaag aaaactgtat
     1741 ggtgcagtac cgttggtcgg tgacaaaaca acctgtgcat cgccagctcc gaaatatgat
     1801 tctgttatga tggttataga actatccggc actgtaattg gaggtggctg cttgatttca
     1861 acgaattgct tcacctcaca acctgcagta ttgactattg tgacgaaata atagcctgct
     1921 ttgagattgg tcagacaaga atcaccggga gtgccgttcc atttataaat atatggtccg
     1981 aaattgcctg ccacctgaac acaagctgta ccttcacttc caccattaca gttgttgtgt
     2041 tctttcgcaa aagttatgct aagtccgcaa tcaggtccgc tcatgaaaat attttgtacc
     2101 aacgtacagc ctttactgtc ggtgaccgtt acggtatata ttccttcacc caatgagtcg
     2161 atggtggtag tagtcgcccc gatattccac agataagtat agggtgctgt accgccggaa
     2221 gcaacgacag atattttgcc attgtttata ttgttattgg tcatgtttgt gttggtgacc
     2281 acaatctgga ggctgtcggg ttgatttatt tcaaagctga atattttatt gcatgaaaga
     2341 gcgtcactga ctgttactgt ataatttccg gctttcagat tatcaagtaa aggtgtggtg
     2401 gcaccggttg accagttgaa gctatatggt gccagcccac cctgtggtgt tatgctgatg
     2461 gtaccggcag aatcaccaaa gcataaaagc gatgttacgt ctgcatctgc cagagtaagg
     2521 ttgtacacag atatgtcgat gcattgttga gttggcggta atgacacatc aaagcaggaa
     2581 ttagcaacat ctacacacaa ttggtggcta cccggagtgg taaagttcaa tttaacagaa
     2641 tttgacatga ggtttggggc acccaccagt ttctcgtcaa gagtccaata ataagcactt
     2701 gcacctttca caggtggaat gctgaacaag acagaatcgc cgatacaaat ttcattgctc
     2761 ttgcttgcta tcggcccaat cggatcatct aatactgccg gagccaactc accattttta
     2821 agctcgatgg tgatatcaca gactgaacca ttgcatccat caagaacgat atagtagagt
     2881 tctcccggaa taagagattt tgttgacagg tttatagaat cagttcgaca caggccctga
     2941 caagcaatgg cttcaaatgg tccaccgcaa ttgcctttat aaatacctgc ctgaaaacca
     3001 aagcttcctg ctgcactcgt acagttgtca gcaagcaaca gaagatccat attattggaa
     3061 gctgcaacga atccaagcca caacgagtta ttcggaactc caccccctga cccactgcac
     3121 ataggaatag gggctgtcgg gttagagaaa tccggtagtt ttgtacagta tccatcgaga
     3181 tcttgcgtaa aacaaaaaac aggtgcagtc tcgcaattaa gacctggttc gaaagggtcg
     3241 ttacactgtg caaatgcgca tacctgtatg taaaatatgc tggtgatggt aactaaaaat
     3301 gtaaataatc ctgatttcat atttattctc ttttatttca acaaattatt ctgttcatca
     3361 atcatttttc tgccaaaatt acgcaccctg tacatttctt ttgggtgcaa tgttcgtaat
     3421 gagttatcaa ttatcccaat tgaatgatat acgtaaaccc atattccgga aaaaccctaa
     3481 caacggtcat tgctttaaaa atcttgcgta caattgtttt gaatcgtgaa aaacagccag
     3541 gtaataaaca ccggatggca aatttccgat aaacaatgga atggtagatg ttgtattttt
     3601 ctcatgcctc cttaccattt cttttccttc tgcacttact attctcagtt gtaccgctcg
     3661 gttatcgggt tgtaaatgtt gtaggtacaa ataatctttc gcgggtacag gaaacagctt
     3721 ccaatccgag cagagtggtt catttgttcc ggttccggat cctttttcta ctacaccaca
     3781 ttcatacttt acacaatcat tgaaatctgt cacggttaca caatagcctc ccggcgaaag
     3841 cttgtcaagt acattaactg aatcgcctgt actccataaa atcttgtaag gaggtgtgcc
     3901 accaacaaca tttaaaatag acttaccgtc gagcaaatcg ggccctgatg tgttgaagat
     3961 atatagaatc agttcaaggc tgtcaggttg ggaaaggtta aaaacaacat ttttactaca
     4021 taccgaatca tcttttacag taagcagata tttacctgcc ttgagattgc tgatggaaga
     4081 agttgtatca ccggttgacc attggaaaga atatggtgga gttccgcccg agacttcaaa
     4141 tccgactgct ccgtttttgt ctccgtaaca tagtagaggc acaagtgttg tgtcagtaag
     4201 taagattcct tcgacctcta tctggaagca cttttgggca ggtaaacttg tgacatcgtg
     4261 acaataattg gcaacatcaa cacaaagcag atgtgtacct gcatctttta tttgaagtga
     4321 aatggaattc gtatcttttt taatgtcacc aaccgacaaa ccatcgagag tccaataata
     4381 agaagtggca cctttaacag gtggcacgct gtatgttacg aaactggatg tgcaggtaag
     4441 gtgattttct ccattaatat tgccaatgga gcccggtggg tctgaggatt ggatgctgcc
     4501 ctgtatcata tctattgttt catcacaaaa cggaccacca catccatcaa tccagagata
     4561 atagagcttc cccggtacaa aattctgact gctcaacaaa taaggggcat ttacacaggt
     4621 gccggtacaa actatctcct tactgtttgg cgggcagccg gtgatgatac cagtttgaaa
     4681 ccccgtatta ccaccaacat cgagacaatt gcccggtatc acttcaatag ccatattgct
     4741 tgatccggct acgaaaccta tccaggctgt attattgggc acaccaccac cgttagaaag
     4801 acataatgga tttgggccgg atgggtttac gacatcctgt aaggtggtac aataaccatc
     4861 caaatcacca acggaacaaa tcaccggtgc tgtttcacaa gaatttcccg gcttaaatgg
     4921 cttattgcac tggccgaaaa gtgcctgtga ctgtattgaa acaagtataa atattgctgc
     4981 gatggtctgt aacattgtct tcataaccaa ttgaattcat tcgtgattta ttaaataaaa
     5041 tacgatcctt gcctattctt cacttccttc agattaatta tccaaagata attttattta
     5101 tataaaattg caacaatatt tccaccattc taaaaataat ttaaaattgc caaataatat
     5161 attttaatat cgcataaatc gtatcaaata tattgaatga agacacatgg attccatcgt
     5221 acagatctaa cttttccatt tcatactgac attgtattcc tttttgtgta aaatagagga
     5281 aataaaaaat gccctatctg atgacaagac agggcatttc tgaatatttt atagtctttg
     5341 ttttatttta tgataaactt cgcgtacaat tgttttgaat gatgatgtag cagtaggtaa
     5401 tattggcctg aaggcaaatg tgcaacactc acaggtatgg taccggctgt atttttctgt
     5461 tcaccggtaa ggatatttgt acctgaagca tccagtattt gccaactcac ttgagagcca
     5521 tcaactatag cttgtttgag gtaaagaaag tcttttgccg gattagggaa aagtttccat
     5581 ccctgcgaaa tcaagtcatt ggtagaaact ttaagtttca cttcggcaca tttgaccaca
     5641 gaacaaccag ctgcatccgt aatggtgaca caataattac cgggtgcaag tccggtaagc
     5701 aattcggtag tactgccatc ttgccaaagg tagctgaatg ttccctgacc accagttgcg
     5761 cttaccaaga cagaaccatc ggtattgccc ggtgtagcat catttatttc gaaatttaca
     5821 aaaatactgt caggctggtc gatatccagg atgagtgtga ataaacagtt tttggcatcg
     5881 gtcactttta atgaagtaag atttttgaaa gtatcggtaa cggtactgtc tgtacttcca
     5941 ttgctccata atatattgta ggccggttgg ccacctgaaa tgttctttat gcttagtgtc
     6001 acaggtttat caaaacaact tgtctgcaca tccaggtcta ttgccttaat accaagcgaa
     6061 tcgcaaggat aggcaggcac aaagaaagga aatataacag aacagccaag agaatccttc
     6121 acttcaacag tgtgattacc tgcctgtaac atagtaccgt cagatgcccc attgtcgtag
     6181 aagacctgat aaggaggcac accacctgac gggcttacca ctaccgaagc atcattagcc
     6241 ccaaagg
//
LOCUS       DAOQQY010000169         6228 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5365_length_6228_cov_5.821643, whole genome shotgun sequence.
ACCESSION   DAOQQY010000169 DAOQQY010000000
VERSION     DAOQQY010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6228
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_6228_cov_5.821643"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4592
                     /locus_tag="PLE23_08585"
     CDS             <1..4592
                     /locus_tag="PLE23_08585"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83358.1"
                     /translation="SGSFTPGEVYYFIIDGCNGSVCDITVDVLQGGPLVMGTINPIAG
                     PKKVCLGGTFNYSVNNVNGATAYHWTLDGNLLSDPETEDNNISITFNDEGVHQLCVDV
                     SNYCNDVAGPPNQQCIDITVEKVVAKDPPEKWVCPNEEYVYNGVPYGVGEHEITLQSW
                     QKCDSVVTLKINEIDIPPKDLGIFYRCKGDCITIEDKYGAGQLLFCDNTESEDVVLKS
                     YQGCDSIVTFTLRLVEVDVKIEPPYELGCVVDQTPLDGSYSYVENYETVEYLWTMCPT
                     CYILGSPTDPEVDTEVPGEYCLTITATAPDGTVCSGKKCVTVTFNPFSPIAGIIGDNL
                     TCAQDSVILQGTSNDPDVTYSWKGPNNQSYTTKDIKVGTPGTYTLTVTSPNLCSNTKT
                     YDVLSFINYPNADASGGLIDCNKPKIQISGKSDTAGVSYAWYNDKNQKISSTAIANVD
                     TVGNYYFEVTNPKNGCVSYDTVAVISDFEQPKDVTASGTTFTCTTFPVKVDANSSTTG
                     VTYTWTGPNGFSSNIKNPDAPYEGDYTVVVTGPNGCKDTAAATIFSDTIKPVVKTFND
                     TIECFTYTATVKASSSAPNSSYSWSGPGASGTGDSLIVNQSGTYTVTVTDNSNGCTTI
                     ATASALDASATPKAIIQTPAILTCDSVTVTIIGGSTLNIPSITFEWNGPGGYTANTKD
                     AKVNLPGTYTLTVKNTDNNCSDNLSVDVDINIAKPNVSALGDTTDCISGTATLCGNSS
                     TPNAKFQWYTATDVALCNTICCDVAGAGDYKLIVTDPVNGCTSQADVKAVKDDDTPDL
                     SFTKSDDLDCNVTSVDLTAISSVTGAIYAWSGPGAPASSVTNYSTGTPGVFTVVVTNP
                     KNNCTNSASVNVLQDIVKPVISAISDTIKCNNNKTATITGTSNVTNNINVQWLDPSGN
                     PVANTLNFTTGNSQIYQLVVTNNTNGCSSTLNLNVPENTKLPDISATGDVITCYEPEA
                     VCNGNSVTPNVSYEWSGPGGFTSSAKNASNITVDGTYQLVITDNINGCSSTTTVNVTK
                     DNSNPDITAAGGTLTCTNNSQISLSANSNTTPIIYKWSGPNNFSSNQQNPVVNVSGSY
                     VVSITNTQNGCESTQSVEVLSDEEKPDLSVNDAVLDCIVTSQTLNALSNTPGVTYQWT
                     GPNTSSTSSSVVVTEAGQYTCVVTAPNGCTTSKTSTVTLNADLPTALAKANGELNCTV
                     KTVTVSSAGSSSGPQFTYTWSGPGGYTSSQPDINAVEAGLYTLVVTNTLNGCSKSITA
                     TVPINENVPTALATESKNPNCFGTTDGSISVKGVTGGTSPFVYSINGKSFTAESKFSF
                     LGEGTYKISIQDAVGCEYDTLLILKQPELLVVDAGKDTIIPWGTTYQIEANITPANAK
                     IASISWNPVVDTFCANCLSPTVTPFDASLYTITVVDSNGCKATDKILILVKKERPVFI
                     PNTFSPNGDGLNDVFYINARDGFIEEILYFQVYDRWGNKIFEKEKFQPNDPAYGWDGS
                     YRNKKVNSAVFVYWALIKFKDGESFLYKGDVTVQR"
     gene            4728..>6228
                     /locus_tag="PLE23_08590"
     CDS             4728..>6228
                     /locus_tag="PLE23_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83359.1"
                     /translation="MFNVCISCLTRTLRPIIRSKQHFNKLSFLLPALVLCIDISHAQN
                     VDCATATTICNDPITMGNPSGMGMNDFEPPGNDPGCLQNESANSSWYYFEFDSSTPPG
                     SIFGFIISPAGGKGEDYDWALYGPDVGCAGLGTPIRCSSSSESCGFCPQTGMGMGATD
                     FSEGPGTGDGFVATLAVQPGQGYFLNINNWKGTGNGFSLEFTGDAAEFLDCGADPPCS
                     LIATALPEKITKCQGDPDFTMSATAFGGLAPYTFMWSGTGNGTGFLDDPDSENPNGTI
                     PPDFAGSITYTVTVSDSKCEATDMVELVVNPKPVVTIEQPPKLCSNGAIYSLIGTPST
                     GTWGGIASLNGKIDPSKLPSGTYQATLSYTNSFGCSDMATVDIEIIDPVEINVDPIPA
                     QCINNPAFVITPDPSGGQWSGNIDPDGEFNPAKFGLGSHTAIYTYYSPEGCKSETSVI
                     IDVIDLPDIDITDPGILCADETIVPLSASPAGGTWSGVADPVGNVYPNQL"
BASE COUNT         1922 a         1342 c         1272 g         1692 t
ORIGIN      
        1 caagcggcag ttttacacca ggtgaagttt attatttcat catagatggt tgtaatggat
       61 cggtatgtga catcacagtg gatgtattac agggtggccc cctagtgatg ggtacaataa
      121 atccaattgc aggacctaaa aaagtatgtc ttggaggaac atttaactat agtgtgaata
      181 atgtgaatgg cgcaacagct tatcattgga cacttgacgg aaatttgctc tcagatcctg
      241 aaactgaaga taataatatc tctattacat tcaatgatga gggtgtacat cagttatgcg
      301 ttgatgtttc caattattgt aatgatgtag ccgggcctcc caatcagcaa tgtatagata
      361 taaccgttga aaaggtggtg gccaaagatc cgcctgagaa atgggtatgc cctaatgaag
      421 aatatgtata taatggtgta ccttatggtg ttggtgaaca cgaaattaca ctccaatcat
      481 ggcaaaaatg tgatagcgtt gtaacattaa aaatcaatga aatagatatt ccgccaaaag
      541 atcttggaat cttctaccgt tgtaaaggtg actgtataac catcgaagac aaatacggtg
      601 ccgggcagtt attattctgc gacaacacag aaagcgaaga cgtcgtactg aaatcctacc
      661 agggttgcga cagtatagtc acatttaccc tacgcttagt agaagtggat gtcaaaatcg
      721 agccaccata cgaactaggc tgtgtagttg accagactcc gttagatggt tcatattcat
      781 acgttgaaaa ttatgaaacc gtagaatatc tgtggacaat gtgccctacc tgttacatcc
      841 taggctctcc tacggatcct gaagtggata cagaagtgcc tggtgaatat tgtctgacca
      901 taaccgccac tgcacctgat ggaactgttt gttctggtaa aaaatgtgtt accgtcacat
      961 ttaacccatt ctcacctatt gcaggcatca ttggtgacaa tctaacttgt gcccaagatt
     1021 ctgtgatact tcagggtact tccaatgacc cggatgtcac ttattcctgg aaaggaccca
     1081 ataatcagtc atacacaacc aaagatatca aagtaggtac tcctggtaca tataccctca
     1141 cagtaacttc accaaatctt tgctccaata ccaaaacgta tgatgttctg tcatttataa
     1201 attacccgaa tgcagatgct agtggtggct tgattgactg taataaacca aaaatacaga
     1261 taagcggtaa aagtgataca gccggtgttt cctacgcatg gtacaatgac aaaaaccaaa
     1321 agatttcaag tacagccata gccaatgttg acactgtagg aaattattat tttgaggtga
     1381 ccaatcctaa aaacggttgt gtgagctatg ataccgttgc agttatttca gactttgaac
     1441 aaccaaaaga tgtaacagcc agcggaacta ctttcacttg tacaaccttc ccagttaaag
     1501 ttgatgctaa ctcatccact accggtgtta cctacacatg gaccggacca aatggattct
     1561 cttccaacat caagaaccct gacgcacctt atgaaggtga ctatacagtc gtggtgacag
     1621 gaccaaacgg atgcaaggat acggctgcag ctactatctt ttctgatacc atcaaaccag
     1681 tagtcaaaac atttaacgat acaattgaat gctttacata tactgcgacc gttaaagcca
     1741 gcagttcagc tcctaattca agttattctt ggtcaggacc tggtgcatct ggtacaggtg
     1801 acagcctgat tgtcaaccaa tccggtacat acaccgtaac ggtaactgat aactcaaacg
     1861 gctgtaccac aattgctaca gcaagtgcgc tggatgcttc tgctacccct aaggctatca
     1921 tacagacacc tgccatactc acttgcgaca gtgttactgt taccataatt ggaggcagca
     1981 ctttgaatat accaagcatc acattcgaat ggaatggccc aggaggttat acagcgaaca
     2041 cgaaagatgc taaggtgaac cttccgggta catatacact tactgttaag aatacagaca
     2101 acaattgttc tgataatctg tcggtagatg ttgatataaa tattgcaaaa cctaacgtat
     2161 ctgccttggg tgatacaaca gattgtattt cgggtacagc aacactgtgt ggtaattctt
     2221 ctactccgaa tgccaaattc caatggtaca cagcaactga tgtagcttta tgcaatacca
     2281 tctgttgtga tgtagcaggt gctggtgact acaaactcat cgtcacagac cctgtcaatg
     2341 gatgtacatc acaggcagat gtaaaagctg taaaagatga tgacactcct gacctgagtt
     2401 ttacaaaatc tgacgatctc gattgtaacg tcacatcagt tgacctgact gccatttcaa
     2461 gcgtaacagg tgctatatat gcatggtcag gccctggtgc accggcaagt tctgttacca
     2521 attattctac cggcactccg ggcgtattca cggtagtagt taccaatcca aaaaacaatt
     2581 gtactaattc agcttctgtc aatgtacttc aggatattgt caaacctgtt atttcagcca
     2641 tttcggatac cattaagtgc aacaataaca aaacagcaac catcacaggt acatcgaatg
     2701 tgaccaataa catcaatgta caatggctag acccaagtgg aaatccggta gccaacactc
     2761 tgaattttac aaccggcaat tcacagattt accagcttgt tgttactaat aacaccaatg
     2821 gttgttcaag tactttaaac ctcaatgtac cagaaaatac aaagttgccg gatatttcag
     2881 caacaggaga tgttatcact tgttatgaac ctgaagcagt ttgtaatgga aattcggtaa
     2941 cacctaatgt aagctatgaa tggagtggtc cgggaggttt tacttcatca gctaaaaatg
     3001 cttcaaacat aacagtcgat ggaacatacc agcttgtaat aacagataat atcaatggat
     3061 gtagttctac tacaacagtt aatgtaacca aggataattc taaccctgat atcactgcag
     3121 ccggtggcac gcttacttgt actaacaatt cgcagatcag cttatctgca aactccaata
     3181 ccactcctat tatttataaa tggtcaggcc ctaacaattt ttcaagcaat cagcagaatc
     3241 ctgttgttaa tgtttcgggc agttatgtag tctctatcac caacactcag aatggttgtg
     3301 aatctaccca atcagtcgaa gtattgagtg atgaagaaaa acctgatttg tctgtgaacg
     3361 atgctgtact tgattgtatc gtcaccagcc agactttaaa tgctttgtca aatacgccgg
     3421 gcgtaacata tcaatggacc ggacctaata caagcagtac cagcagctca gtggttgtta
     3481 ctgaagcagg gcaatataca tgtgttgtaa cagcacctaa cggatgtaca accagcaaaa
     3541 catcaacggt aacgctcaat gccgatcttc cgacagcact ggccaaagca aacggtgaac
     3601 taaactgtac agtcaaaacc gtaactgtaa gttcagcagg atccagcagt ggtccacaat
     3661 ttacttatac atggagcgga ccgggtggat atacatcttc acagccagat atcaatgcag
     3721 tggaagcagg tctatacacg ctggtagtaa ccaatacact caatggttgc agcaagtcaa
     3781 taacagctac ggtaccaatc aacgaaaatg taccaactgc cttggctact gaatccaaaa
     3841 acccgaattg ttttggtaca accgatggtt ctatttcagt aaaaggtgtc acaggcggaa
     3901 cttcgccgtt tgtttattct atcaacggaa aatcttttac cgctgaaagc aaattttcat
     3961 tccttggaga aggtacttac aaaataagta ttcaggatgc agtaggttgc gaatacgata
     4021 cactactcat actgaaacaa ccggagttac ttgttgtaga tgccggaaaa gacactatca
     4081 ttccttgggg cacaacctac cagatagagg ctaacatcac acccgctaat gcaaaaatag
     4141 cttccattag ctggaatccg gttgtcgata ctttttgtgc aaattgtctt tctccaaccg
     4201 tgacaccatt tgacgcaagc ctatacacta ttacagtagt ggacagtaac ggttgcaaag
     4261 cgacagataa aatcttgatt ttggttaaaa aagaacgtcc ggtgttcata ccaaatacat
     4321 tttcaccgaa tggcgatggc ctgaatgatg tgttttatat caatgctcgt gatggtttca
     4381 ttgaagaaat cttgtacttc caggtatatg accgatgggg aaataaaatt tttgaaaaag
     4441 aaaaattcca accaaatgat cctgcctacg gatgggatgg ctcgtaccgc aataaaaaag
     4501 tgaacagtgc agtcttcgta tattgggcac ttatcaaatt caaagacgga gaatccttcc
     4561 tttataaagg agatgtaacc gtacagagat aaattattaa ataaataaat atcaaggcct
     4621 gggcaaaatt gttcaggctt tttttgtgaa attgaaatat aaattcataa ttttgaaata
     4681 gcaaactcac atttttttat aacaaccaat cattcaactt taatacaatg ttcaatgtat
     4741 gtattagttg cctcacgcgt acacttaggc caataataag atctaagcaa catttcaata
     4801 agctgagttt cctattgccg gcactggtat tatgtataga tatttctcat gctcaaaatg
     4861 tagattgcgc cactgccacc accatttgca acgacccaat aaccatgggc aatccttcag
     4921 gaatgggaat gaatgacttt gaaccacccg gcaatgaccc tggatgtctc cagaatgaaa
     4981 gtgccaactc gagttggtat tattttgagt ttgacagctc aacaccaccc ggttctattt
     5041 ttggtttcat catttcgcct gccggtggaa agggagaaga ttatgactgg gcattgtatg
     5101 gccctgatgt cggatgtgcc ggccttggta cacccatcag atgctcatca tcaagcgaat
     5161 catgtggctt ttgtccacaa accggaatgg gcatgggtgc aaccgatttt tcagaagggc
     5221 caggcacagg agacgggttc gtagcaacat tggcagtaca acccggacaa ggctactttt
     5281 taaacatcaa caactggaag ggcacaggca atggtttcag tctggagttt acaggtgatg
     5341 cagcagaatt tcttgattgt ggagctgacc caccttgctc gcttatagcg acagctttac
     5401 ctgaaaaaat taccaaatgc cagggcgatc cggatttcac gatgtctgca actgcctttg
     5461 gaggccttgc cccttataca tttatgtggt caggtacagg taacggaacc ggattccttg
     5521 atgacccgga ttctgaaaat cccaacggga ctatacctcc agatttcgca ggttcaataa
     5581 catatacagt aacagtatct gacagcaaat gtgaggctac tgatatggta gaactggttg
     5641 taaatcctaa accagtcgtc accatcgaac aaccacccaa gctctgttcg aacggtgcta
     5701 tatattcctt aatcggaaca ccttctaccg gtacatgggg aggtattgct tcattgaacg
     5761 gtaaaatcga tccgtctaag cttccatctg gtacctatca ggcaacattg tcatacacca
     5821 attcattcgg atgttcggat atggcaacag tagatatcga aatcattgac ccggtagaga
     5881 taaatgttga tccgatccct gcccaatgta taaacaatcc tgcatttgtg ataacacctg
     5941 acccatcagg agggcaatgg agtggcaata ttgatccgga tggtgaattt aaccctgcca
     6001 agtttgggct tggtagtcat actgctattt atacttacta cagccctgaa ggttgtaaaa
     6061 gtgaaacctc agttatcata gatgtcattg atcttcctga tattgacatt accgaccctg
     6121 gtatcttgtg tgctgacgaa accattgtac cactttctgc ctcaccggcc ggaggtacgt
     6181 ggtcaggtgt agcagacccg gtaggtaatg tttatcctaa tcagctgg
//
LOCUS       DAOQQY010000170         6220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5376_length_6220_cov_5.792052, whole genome shotgun sequence.
ACCESSION   DAOQQY010000170 DAOQQY010000000
VERSION     DAOQQY010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6220
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5376_length_6220_cov_5.792052"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PLE23_08595"
     CDS             <1..1256
                     /locus_tag="PLE23_08595"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMX83360.1"
                     /translation="NPEYLHRTVKNYTDIIISDLFSPPVSSRNYTYASIAAYEVLRNL
                     KPGYASLKGQLKDFPDIPAVPQGQTICFPVAGIQAYYATGKKFIFSEPDWTDKEETIL
                     KEMKALGIPQDVYDHSIAYGNAVADAIKTWADGDNYKETRSMPKHTTNYKEGHWRPTP
                     PDYNEALEPNWYKMRSFVFDSTNMVECEPHIPFSKNTKSTFWDNASEVMSIGNQLKPE
                     QRDIAIFWDDNPVVTMHSGHAIYDNKKMTPGGHWLNVVRIASEKAKVDMLTASEAYMY
                     VSLGLYEAFRGCWLAKHKSDLIRPESYINQYIDPKWKPLLQTPPFPEHSSGHSTISAA
                     SAEICTYIFGDNFAYTDNTEEEYGNGTRSFTSFYQAALEASLSRVYGGIHYRHGCDSG
                     NRHGLKIGKFILDNVKTRPSAVGMK"
     gene            1315..2643
                     /locus_tag="PLE23_08600"
     CDS             1315..2643
                     /locus_tag="PLE23_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMX83361.1"
                     /translation="MTELKRKLSLYGLTMIAVGSCIGSGIFISPADVVQSIPNPEYAL
                     LAWMVGGIIALTGSLSYSELGSMFPQSGGVYVYLKEAFGEQVAFLYGWVTLLVINTGA
                     LAALSLAFVNYLTFFVPLDDTLKLGIAVATILILTAINTRDVGISQWFANIFTGIKLA
                     VLVAMIIAGAVIFGDPERVFSSGNTFSIIGQPGAFLTALIGILWSFGGWHHASYLAGE
                     AIDPNRNVPKSMIFGTLIVTVVYVLTNFAYFSLLSVEQFAASDRVAGDAMATIFTNGG
                     RMVSLGVMISVFGTIGIYTMSAPRIYHSMAQDGIFFKKLAKLHDKNGTPAFAMWFQAI
                     WASLLIILWRTFSDLVTYTTIMDIAFLALAGFSLFVLRYKKPHLERRVRVPLYPLVPA
                     IFVLISALFILNALAEKPQQAVAGIAVLGVGLIVYRYFKNKKTNHGKDNI"
     gene            2740..3024
                     /locus_tag="PLE23_08605"
     CDS             2740..3024
                     /locus_tag="PLE23_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX83362.1"
                     /translation="MKIEEIIREKKGTILDVRTYSEFSGGHVAGSKNIPLDQVPEKLS
                     EIKSLEKPLLLVCASGNRSGQATQYLTSLGVECMNAGSWMDVNYYQSLNS"
     gene            3028..3345
                     /locus_tag="PLE23_08610"
     CDS             3028..3345
                     /locus_tag="PLE23_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX83363.1"
                     /translation="MLQFIKNLLGMGPKTDFAQLVKSGAIILDVRTANEYKMGHIKGS
                     VNIPLNSLEGHVRKLKEKGKPIITCCASGMRSASAKSLLVSGGITDVYNGGGWQSLRS
                     RIN"
     gene            complement(3671..3991)
                     /locus_tag="PLE23_08615"
     CDS             complement(3671..3991)
                     /locus_tag="PLE23_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMX83364.1"
                     /translation="MNIFVAKLNYDTNDDTLRSTFEQYGAVDSAKIVMDKFTGRSKGF
                     GFVEMPDDSEAMNAINGLNDTELDGRTIVVKKAEPRENSGRDNFRGGNDRRGGFRNDH
                     DRRY"
     gene            complement(4216..4863)
                     /locus_tag="PLE23_08620"
     CDS             complement(4216..4863)
                     /locus_tag="PLE23_08620"
                     /inference="COORDINATES: protein motif:HMM:NF017534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMX83365.1"
                     /translation="MANITVIYSTRTQYSLMKKAMDITHNKSFWDNKYSNNDTGWDIG
                     SPSTPLKEYIDQIQNKNIKILIPGCGNSYEAEYLLDSGFSDITLIDIADKPISLLRQT
                     FKDRKEIHIVHGDFFKLSGQYDLILEQTFFCAIDPSLRSEYVNHAISLLNPNGKLAGV
                     MFNCDFNKEGPPYGGNLEEYKKLFEEQFVIRTMTPCYNSIPPRSGNEVFIIFEKA"
     gene            complement(4925..5680)
                     /locus_tag="PLE23_08625"
     CDS             complement(4925..5680)
                     /locus_tag="PLE23_08625"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HMX83366.1"
                     /translation="MKERLVVLALLLSSALQAQIVNMEEQRIVTNTTGWAGELKFGLT
                     TSKEVQQVLNLITENQLQYKTKKDLYLIRLNSKILKTNANTITDNTFIHFRYNRKFSD
                     LLRLEAFTQWQHNRITGIKRRYLIGAGPRFKVLETKNFNSYIGLLGMYEYKLEYSPEA
                     SITPLQVIRSSNYTSFTWRILPNIKLINTTYYQPRPDLLNDYRVFSQASLQFGITKRL
                     SFEFGGWYLFDNVPAPGVPKKTYAIENFLKWKF"
     gene            5825..>6220
                     /locus_tag="PLE23_08630"
     CDS             5825..>6220
                     /locus_tag="PLE23_08630"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMX83367.1"
                     /translation="MQLIIILGALALLFFYLVSIYNRLVRLRTMVSEGWSGIDVQLKK
                     RHDLIPNLLESVKGYKNFEQETLQKVIAARNAAMGAKDVASHAAAEGQLQRAMGGFFA
                     LAEQYPDLKASANFIQLQDSLKEIETSIEQ"
BASE COUNT         1862 a         1230 c         1242 g         1886 t
ORIGIN      
        1 ccaacccgga atatctgcac agaacagtta agaattatac agatatcatc atttccgact
       61 tgttttcacc accggtgtcg agcagaaatt atacttatgc ttctattgca gcatacgagg
      121 tattgagaaa cctaaaaccc ggatatgcaa gtctgaaagg tcaattaaaa gattttccgg
      181 atataccggc agtaccacaa ggtcagacaa tttgtttccc tgtggctggt attcaggcat
      241 attatgcaac cggcaagaaa tttatttttt ctgagcccga ctggactgat aaagaagaaa
      301 ctatactcaa agaaatgaag gcacttggta ttcctcagga tgtatatgat cattccattg
      361 cttatggcaa tgctgtagct gatgccatca aaacctgggc agatggcgac aactacaaag
      421 aaaccagatc aatgccgaaa cacactacca attataaaga aggccactgg cgcccaacgc
      481 cacccgacta caatgaggca ctcgagccca actggtacaa gatgcgttca ttcgtatttg
      541 actctaccaa tatggttgaa tgtgagccac atataccttt tagcaaaaat acaaagagta
      601 ctttctggga caatgccagc gaggtaatgt ctataggaaa tcaattgaaa cctgaacagc
      661 gcgacatagc catcttctgg gatgacaacc cggtagtaac gatgcacagt gggcatgcca
      721 tttatgacaa taaaaagatg acaccaggcg gacattggct gaatgtcgta aggatagcaa
      781 gcgaaaaagc aaaagtggat atgcttacag caagtgaagc ttacatgtat gtatcacttg
      841 gactttatga ggcctttaga ggctgttggt tagcaaagca caagtcggat ctcatcagac
      901 cggaatcata tattaaccaa tacatagacc ctaaatggaa accacttctg cagactccac
      961 ctttcccgga acactcctcc gggcacagta caatctctgc agcatctgct gaaatatgta
     1021 cctatatttt tggagataat tttgcatata ccgacaatac tgaagaagaa tatggcaacg
     1081 gcacccggtc gttcacttca ttttatcagg ctgccttaga ggcatctttg agtcgtgtgt
     1141 acggaggcat ccattacagg cacggatgcg attcgggaaa caggcacgga ctgaagattg
     1201 gcaaattcat tcttgacaat gtaaaaacaa ggccaagtgc cgtaggaatg aaataattct
     1261 tccggtaatc cggaaatttt ttaatacttt tagctttcaa ttctaaccaa cactatgaca
     1321 gaattaaagc ggaaactatc actgtacggt cttactatga ttgccgtagg atcgtgtatc
     1381 ggttcaggta tatttatttc acctgctgat gtagtacaaa gcattccaaa tccggaatat
     1441 gctttacttg cctggatggt aggtggcatc atagccctta caggttcact tagctactca
     1501 gagcttggct ctatgttccc ccaatcgggc ggtgtctatg tatatcttaa ggaagcattt
     1561 ggcgagcaag ttgcatttct ttacggctgg gtcactttac ttgtcatcaa caccggtgct
     1621 ttagctgcac tttcgttagc atttgttaat taccttacct tttttgttcc actggatgat
     1681 acgctcaagt taggcattgc ggtagctacc atacttatac tcacggctat caacacaaga
     1741 gatgtaggca tcagtcaatg gtttgccaat atttttacag gtatcaagct agctgtattg
     1801 gtggcgatga taattgccgg agccgtaata tttggagatc cggagcgtgt tttttcgtcg
     1861 ggcaacactt ttagcattat tgggcagccc ggagcatttc ttacagctct tattggcatc
     1921 ttgtggtctt ttggcggctg gcatcatgct tcgtatctgg caggtgaggc tattgaccca
     1981 aaccgaaatg tacctaaatc catgatattt ggcacactca tcgtcacagt tgtctatgtc
     2041 cttaccaatt ttgcctattt ttcacttctg agtgtagagc agtttgcagc atcagaccgt
     2101 gtagccggtg atgcaatggc cactatattt accaatggag ggcggatggt atcgctcggt
     2161 gtaatgattt ccgtatttgg caccatcggt atttatacaa tgtctgctcc gcgtatctat
     2221 cattcgatgg cgcaggatgg tattttcttt aaaaaacttg ccaagctgca tgacaaaaac
     2281 ggaacgcctg catttgctat gtggtttcag gccatttggg catctttact tattatactc
     2341 tggcgcacat ttagcgactt ggtcacttac acaaccatta tggatattgc atttttggca
     2401 ctggccggtt tcagcttatt tgtactgaga tataagaaac cacatcttga gcgacgtgta
     2461 agggtaccgc tttatccatt ggtgcctgct atttttgttt tgatttctgc attgttcata
     2521 ctcaatgcgt tggctgaaaa accgcaacag gctgttgcag gtatagctgt gctgggtgtt
     2581 ggcttgatcg tttaccgata tttcaaaaat aaaaaaacca accacggcaa ggataacatt
     2641 taaagccgtt ttttactaca ataatctatt gtaactttag ttaccataag cgcggttcag
     2701 gtgcatgtat ctttgcatca acaaaatatc aatattaata tgaaaatcga agaaatcatc
     2761 cgagaaaaaa aaggaaccat ccttgatgta cgtacataca gtgaattttc tggcggtcac
     2821 gtagcaggtt ctaagaatat tccactagat caggttcccg aaaaactttc agaaatcaaa
     2881 agtttggaaa aaccactgtt gctggtttgt gcatcaggta accgaagtgg gcaggcaacc
     2941 cagtatctga cttctctagg agtagaatgt atgaatgccg gctcatggat ggatgtcaat
     3001 tattatcaat cactaaactc ttagtctatg ctacagttca tcaaaaacct tctaggcatg
     3061 gggcccaaaa ctgatttcgc ccaattggta aaaagtggtg ccattatcct ggatgtacgt
     3121 actgccaacg aatacaagat gggtcacatc aaaggttcgg taaatattcc acttaacagt
     3181 ttggaagggc atgtgagaaa attaaaagaa aaaggaaaac ctatcattac atgttgtgca
     3241 tccggcatgc ggagtgcaag tgccaagtct ctcttggtct caggcggtat tactgatgtg
     3301 tacaatggcg gtggctggca aagccttcga tcaaggatta attagaaaat tttggtttat
     3361 aaatctaagc cctgctgtca gaaagtaggg cttttttgtt tttattgaac ccggataacg
     3421 aaataggctc tttactgcaa ataattttta cttcaaatcc atttcaaaag ctcaaaaacc
     3481 atcgttcact atacttttat ctattcttta tagatttttt atactaatca ttttcgttta
     3541 aagtcctaat gtgatagctg ggttaaaata cagaccaaag attgaaggca gcacagaata
     3601 attttcatga aataaaaaag cggaaaatcc gataaaggaa atcccgcttt tttcttaact
     3661 aagaagttta tcaatatctt ctgtcgtggt catttctgaa accgcctctg cgatcgttac
     3721 caccacggaa attgtcacgt ccgctgttct ctcttggttc agctttttta acaacgatgg
     3781 ttctaccgtc aagttcggta tcattcaggc cattaatagc attcatagct tcagaatcat
     3841 caggcatttc aacaaaaccg aatcctttag aacggcctgt aaatttgtcc attacgattt
     3901 tagctgaatc aacggcaccg tactgttcga atgtacttct gagcgtgtca tcattagtgt
     3961 cgtagtttag ttttgcaaca aaaatgttca tcaaaataaa aattaaaaag gttaaaaaaa
     4021 aatgagaaat actgcgatag aaaaaaatcg atgaacgaaa taaactaaac gaaggatttt
     4081 actcaaaaac atggcaaata tataacatat tctattgggt tgccatattt attttataaa
     4141 tatgtctaaa atgttaaaat aacttgtatg acaatcagat tttaggataa aaaatagttg
     4201 cagtaacaaa caatctcaag ctttttcaaa tattataaaa acttcattcc cagatcgtgg
     4261 aggtatggaa ttataacatg gtgtcatagt ccggatgaca aattgctcct cgaacagttt
     4321 tttatactct tctaaatttc cgccgtaagg tggtccttcc ttattaaaat cacaattgaa
     4381 cattacgcct gcaagctttc cattcgggtt caataaacta atggcatgat tgacatattc
     4441 actacgcaag gatgggtcaa tcgcacaaaa gaatgtttgc tcgagtatca ggtcgtattg
     4501 gcccgaaagc ttgaaaaaat ctccgtgtac aatgtgtatt tcttttctgt ctttaaatgt
     4561 ttggcgcaat aagcttattg gtttatccgc aatatcaatg agggtgatat ctgaaaatcc
     4621 ggaatccaac aaatactcag cttcatagct gttaccacat ccgggtatca gtatctttat
     4681 atttttattc tgtatctggt caatatactc cttaagcgga gttgagggtg aaccaatgtc
     4741 ccagccggta tcgttgtttg aatatttatt gtcccagaaa gacttgttgt gtgtgatatc
     4801 cattgctttt ttcattaaac tgtactgtgt acgtgtacta tatattactg taatgtttgc
     4861 caatgatttt tattgtgtat gcagattaat ttctgataaa ccaaatatct ccataaggta
     4921 tgccttaaaa tttccatttt agaaaatttt caatagcata agttttcttg ggtacgccgg
     4981 gtgccggaac attatcaaac aaataccagc caccaaactc aaaactcagt cttttggtaa
     5041 tgccaaactg caatgatgcc tgactaaaca cacggtaatc attcaataaa tctgggcgag
     5101 gttggtaata agtggtattg atgagtttga tattgggcaa tattctccag gtaaaagatg
     5161 tataattact cgaccgtatt acctgaagcg gtgttatact tgcttcgggg ctgtattcca
     5221 gcttatattc gtacatgccg agaagcccga tatacgagtt gaaatttttt gtctccaata
     5281 ccttgaacct tgggcctgca cctatcagat accgacgttt gataccggtg atacggttat
     5341 gctgccattg ggtgaatgcc tcaagcctta aaagatcact gaacttgcga ttgtatctga
     5401 aatgaataaa tgtattgtcg gttattgtat tggcattcgt cttgagtatc tttgaattga
     5461 gccttatcaa gtagaggtct tttttagttt tgtactgcag ctgattttca gttatcaggt
     5521 tgagtacttg ttgtacctct tttgatgtag tcaggccaaa tttcaactct cctgcccaac
     5581 cggtggtgtt ggtcactatg cgttgttctt ccatatttac aatctgagct tgcagcgcgc
     5641 ttgaaagtaa cagtgccaga acaaccaatc tttctttcat ttatttcttc ttgttatttc
     5701 caaaattaaa taagtcattt aagcaaaaaa tgaacattat taataaaaaa acggaattgt
     5761 ttttcgctgt aaaatgtttt acctacattg cattcaattt gtttcaccta aaatctaata
     5821 aaccatgcaa ctgattatta ttctcggtgc gcttgcactg ctatttttct atctggtaag
     5881 catttataac cgacttgtaa ggttaaggac aatggtgagc gaaggctgga gcggaataga
     5941 tgtacaactc aagaaaaggc acgacttgat accaaactta ttggaatcag taaaaggata
     6001 taaaaatttt gagcaggaga cattgcaaaa agtaatcgct gccagaaatg ctgccatggg
     6061 tgccaaagat gtagcatcgc atgctgcagc agaaggacaa cttcagcggg caatgggcgg
     6121 attctttgca ttggcagaac aataccccga tctgaaagca agtgccaatt tcattcagct
     6181 acaagattca ctcaaagaaa tcgaaacatc tatcgaacag
//
LOCUS       DAOQQY010000171         6159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5458_length_6159_cov_7.041448, whole genome shotgun sequence.
ACCESSION   DAOQQY010000171 DAOQQY010000000
VERSION     DAOQQY010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5458_length_6159_cov_7.041448"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..2133
                     /gene="uvrB"
                     /locus_tag="PLE23_08635"
     CDS             118..2133
                     /gene="uvrB"
                     /locus_tag="PLE23_08635"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMX83368.1"
                     /translation="MNFKLTSNYQPTGDQPQAIQKLVNGLNRGETDQVLLGVTGSGKT
                     FTIANVISQLNRPTIVLTHNKTLTAQLYGEFKEFFPDNAVEYFVSYYDYYQPEAYISV
                     SDTYIEKDLSINAEIDKLRLRATSTLLSGRRDIIIVASVSCIYGMGNPEDYKNSIIRI
                     QEGQKITRNSFLHSLVESLYSRSENLQDRGTFRVRGDTVDINLPYLDYGYQVVFFGDE
                     IEEINKINTENGRRFERINHSAIFPANLYVAPRDRIHLIIKQIEDELHEQVKYFEREF
                     QFSEAQRIKERTNLDIEMLKELGYCSGVENYSRFFDGRRPGTRPFCLLDYFPEDYLMV
                     IDESHVTIPQIRGMWGGDRARKQNLISFGFRLPSAMDNRPLNFDEFRKLTYQNVFISA
                     TPGDYELELTQGEIVEQIIRPTGLLDPPIDVRPSINQIDDLLEEISERIKRKERTLVT
                     TLTKRMAEELTKYLQKLNIKVQYIHSDVDTMDRVEIIRDLRLGDIDVLVGVNLLREGL
                     DLPEVSLVAILDTDKEGFLRNDRSLTQTAGRAARNANGLVIFYADKVTDSMRKTMDET
                     NRRRAIQMKYNEENGITPTTVSRTREEILNQSSILDIRGKKAYVEPTEVNIAADPVVE
                     YMDRNGIEKLILETEKKMKAAAKDLDFIQAAQHRDEMYALKKLLERK"
     gene            complement(2376..3641)
                     /locus_tag="PLE23_08640"
     CDS             complement(2376..3641)
                     /locus_tag="PLE23_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HMX83369.1"
                     /translation="MTKPDDIRSKMKWFSDAKLGIFIHWGIYSVNGVGESWSFHNKEI
                     AYDEYMKQINGFTASKYDPAYWASLVEKSGAKYAVITTKHHDGVALFNTQFKERSIPE
                     HSPAGRDLITPFFNELRNRNLKCGAYFSLLDWSHPDYPGFLKDSSRYVIKDNPAKWTR
                     FLDFNNTQIAEISSQLNPDLYWFDGDWEHIADEWHAADLRQMILARNPDAIINGRLAG
                     YGDYDTPEQNIPITRPVYPWWELCMTINNQWGWQPNDKAWKSPYEIITLFVDVIGVGG
                     NLLLDIGPKEDGSIPEEQINVLTELGKWISRNKESVYGTTAGLPNGFFHGASTLSADS
                     TELFLFVPGKDDVQVVLKGLNNKILSANILGTNSTVIPKIVGKISWSTVPGRVFLSIP
                     SAVRDPYMTVIKLKLDGKISLYTGRGGFH"
     gene            3817..4446
                     /locus_tag="PLE23_08645"
     CDS             3817..4446
                     /locus_tag="PLE23_08645"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMX83370.1"
                     /translation="MKAHSKCILIDNHDSFVFNLYRYLNELMPGAILVMKNDEVDFDK
                     IRKAEMIVISPGPDIPKNAGRLMDVLLEFHDKKPILGICLGHQAIIEFFGGKLIKLKT
                     VYHGIQSKIHVNDPDDILYKDIESPFKAGRYHSWSADKESLPTDLIITTTDEDRQIMG
                     ISHATYSVHGVQFHPESIMTPSGRKILSNFVHNHKALTFIEQSKTMSER"
     gene            4488..6017
                     /gene="gpmI"
                     /locus_tag="PLE23_08650"
     CDS             4488..6017
                     /gene="gpmI"
                     /locus_tag="PLE23_08650"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMX83371.1"
                     /translation="MAKKTALLILDGWGIGSRPAADAILQAHTPFFDMLWSRYPHNTL
                     TTFGEAVGLPDGQMGNSEVGHMNIGAGRVVYQELVRINLAFKNGLMTDNIVLCEAAEY
                     SLTRGTSVHIMGLVSNGGVHSHIDHLVSLVEIMAAKGVKNIYIHAFTDGRDTDPHSGL
                     GFLTEAELKTAGKARIVTVIGRYYAMDRDKRWERIKVAYDALVNAKGEMTDDIFTKVK
                     ESYASDTSDEFIRPILLNTKDYEAYHTRIKDGDVVINFNFRTDRPREITTVLTQADMH
                     EYNMHKMDLKYISMTEYDSTFKNIEILFRTEKLEKTLGEVIAVNGLSQLRIAETEKYP
                     HVTFFFNGGRELAFEKENRRLIPSPKVATYDMQPEMSANGIKEAVIEEINHNQPDFIC
                     LNFANTDMVGHTGVFLAAMKAAETVDMCLSEIVRIALAKGYDLFIIADHGNADNMINE
                     DGSVNTQHSMNPVPVIFVSNEIKYTKIKQGKLADIAPTILVNMGVQLPEDMTGENLLE
                     E"
BASE COUNT         2128 a         1119 c         1244 g         1668 t
ORIGIN      
        1 cagaattgaa taaatgaacg acaaatgtaa accaaaataa aaaattcgta gcaacctgac
       61 cagcgacatt caaaatactg agtagatttt gtgcatattc ctatgtaaat ttgtagaatg
      121 aatttcaagc taacatccaa ttaccaacct acaggtgacc aaccgcaagc tatccaaaaa
      181 cttgtaaacg gacttaaccg aggcgaaacg gatcaggtat tgcttggcgt aacaggatcc
      241 ggaaagacct ttaccattgc caacgtcatc agtcagttga acaggcctac catcgtactt
      301 acacataaca agacacttac tgcacaatta tatggagaat tcaaagaatt tttcccggac
      361 aatgctgtag agtattttgt ctcttactat gattattatc agcctgaagc ctatatatca
      421 gtttcggata cttatattga gaaagattta tcgataaatg ctgagataga caagctaagg
      481 cttcgggcta catctacttt gttgtcgggc agacgtgaca ttattattgt tgcttctgtt
      541 tcgtgtattt atggtatggg caatccggaa gattacaaaa acagcatcat acgcatacag
      601 gaaggtcaaa aaataacgag aaactcgttc cttcattccc ttgtagaaag tctttattcg
      661 aggtcagaaa atctgcagga cagaggaaca ttcagagtac gaggcgatac tgttgacatt
      721 aatctaccat atttggatta tgggtatcag gttgtatttt ttggggatga aattgaagaa
      781 ataaacaaga taaataccga aaacggaagg agatttgaac ggataaatca ttctgccatt
      841 tttcctgcaa atctatacgt agcaccacgt gatcgtattc acttaattat caagcagata
      901 gaagatgagc ttcatgagca ggtaaaatat tttgaacgag agtttcagtt ctcagaagca
      961 cagagaatca aggaacgaac caaccttgac atagagatgc ttaaagagtt gggttattgt
     1021 agtggtgttg aaaattactc gaggtttttt gacggtcgca gaccagggac ccgaccattt
     1081 tgtttacttg attattttcc tgaagattat ctgatggtaa ttgatgagag ccatgtgacc
     1141 attccgcaaa taagaggaat gtggggagga gaccgtgcaa gaaagcaaaa tctgatatca
     1201 tttggtttca ggctaccttc ggcaatggat aacagaccac tcaattttga tgaatttcgc
     1261 aaactcactt accagaatgt atttatttct gccacacctg gtgattatga acttgagctt
     1321 acccaaggtg agatagtaga gcaaataatc aggcctaccg gcttactcga tccaccgatt
     1381 gacgtaaggc cgagtattaa ccagatagac gatttgctgg aggaaatttc ggagagaata
     1441 aaaagaaaag aacgaacact ggtgaccaca ctcactaaga gaatggcaga agagctcaca
     1501 aaataccttc aaaaactcaa catcaaggta cagtatatcc actcagacgt ggacacaatg
     1561 gacagggtag aaatcatacg tgacctccgg ctgggcgaca tcgacgtgtt ggtaggtgtt
     1621 aatctgcttc gtgaaggact cgatttgcct gaagtatcgc tcgtggctat tcttgacacc
     1681 gataaagaag gttttctccg caatgaccgt tcactgacac agaccgccgg acgagcagca
     1741 agaaacgcga atggattggt cattttttat gctgataaag taaccgactc tatgcgcaaa
     1801 acaatggatg aaacaaacag aagacgggcc atacagatga agtacaatga agaaaacggc
     1861 atcactccta ctactgtatc tagaacccgt gaagaaattc tgaatcaaag ctcaatttta
     1921 gatataagag gcaagaaagc ctatgttgaa cctacagaag taaacatagc agctgacccc
     1981 gtagtagaat acatggatag aaacggtatt gaaaaattaa tattagaaac agaaaagaaa
     2041 atgaaagcag cagcaaaaga tcttgatttc atacaggctg ctcaacaccg tgatgaaatg
     2101 tatgcgctca aaaaacttct ggaaagaaaa tgagaagatt tactgtaaaa aaaagagagg
     2161 ctaagtacct ctctctgaaa tgattcattt taaccaacaa aatgaattaa acaaacacta
     2221 ttatctttta aaacaaaatt cagtaaatct ttatgtaata ataattacag tacaaagatg
     2281 cgattatttt tttaatcttc ctatgactta ggtcataatt cattaggatt attgtcaatt
     2341 ttccataaaa cattgtaaaa tcaaaggatt tgttttcaat gaaagcctcc ccgacctgtg
     2401 taaaggctaa ttttaccatc cagttttaat tttattacag tcatataagg gtctcttact
     2461 gcagaaggaa tgcttagaaa tacccggcca ggtacagtac tccaggatat tttgccgacg
     2521 atctttggta taactgtgct atttgtacca agtatatttg cagacaatat tttgttatta
     2581 aggcctttta aaacgacctg aacatcatcc tttccgggca caaacagaaa tagctctgtg
     2641 ctatctgcac tcaatgtaga ggcaccgtga aagaatccgt ttggcagccc ggccgttgtt
     2701 ccatagacag attccttatt tcgtgatatc catttgccaa gttcagtcag aacgttgatc
     2761 tgttcctctg ggatacttcc atcttcttta gggccaatat cgagcaagag atttccgcct
     2821 acgcctataa catcgacaaa tagtgtaatg atttcataag gtgatttcca ggctttgtca
     2881 tttggctgcc aaccccattg gttgttgata gtcatacaca gctcccacca gggatagaca
     2941 ggtcttgtaa tcgggatatt ttgctcaggt gtatcataat ctccataacc ggcaagtctt
     3001 ccattgataa tggcatccgg attacgagcc agtatcattt ggcgtaaatc tgcagcgtgc
     3061 cattcgtcgg caatgtgttc ccagtcaccg tcaaaccagt agagatcggg atttaattgg
     3121 cttgatattt cagcaatttg tgtgttgtta aaatcaagga atcttgtcca ttttgcagga
     3181 ttgtctttaa taacgtatcg cgaactatct ttcaaaaacc cgggataatc cggatgcgac
     3241 caatcgagta atgaaaaata ggcaccacat ttcagatttc tatttctaag ttcattaaaa
     3301 aaaggagtaa tgaggtcacg gccagcgggt gaatgttcag gaatgcttct ttctttaaac
     3361 tgcgtgttaa acagagcaac tccgtcatga tgcttggtag tgataacagc atatttcgcc
     3421 cctgattttt caaccagcga agcccaatac gctgggtcgt atttagaggc cgtaaatcca
     3481 tttatttgct tcatatattc atcataagct atttctttat tgtgaaaaga ccatgattcg
     3541 ccaacaccat tgacggagta aataccccaa tgaataaata taccaagttt tgcatcactg
     3601 aaccatttca tttttgaccg gatgtcatca ggttttgtca tttgttgggc aaatgagtct
     3661 ggtaacattg tcaaaaatga gatggcaatt agaatgaaat gaataggttt tattgaaaat
     3721 ttcatcatac atcaagtaaa ttaaatagca attctataca aatctattca atagatgcag
     3781 aaaatcaata attttgttag aatttaaagc aataacgtga aagcccattc caaatgtatt
     3841 ctgatagata accacgattc attcgtattt aatctgtaca gatacctgaa cgaattaatg
     3901 cctggagcaa tacttgtaat gaaaaatgat gaagttgatt ttgacaaaat tcgcaaagcc
     3961 gaaatgattg tcatatcacc cggtccggat attccgaaga atgcaggaag attgatggat
     4021 gtgttattag aatttcacga taaaaaacca atattgggta tatgtctggg ccatcaggca
     4081 attatcgaat ttttcggagg caaattaatt aaacttaaga cagtatatca cggcattcaa
     4141 agtaaaatac atgtaaatga tccggatgac attttataca aagacattga atctccgttc
     4201 aaagccggaa gataccattc ctggtcagcc gataaagaaa gccttcctac agaccttatc
     4261 ataacaacaa cggatgagga caggcaaata atgggtattt cgcatgccac atattctgta
     4321 catggcgtcc aattccatcc ggagtcaatt atgacaccaa gcggaagaaa aattctgagt
     4381 aattttgtac acaaccataa agcccttaca tttatagagc agagcaaaac aatgtccgaa
     4441 agataatcga atgtttagta ttaaaatttg tagaaaaaag aaagaaaatg gcaaaaaaaa
     4501 cagctttact gatactggat ggttggggaa taggatcacg accagccgca gacgcaattt
     4561 tacaggccca cacgccattt tttgacatgt tgtggagtag atatccacac aatacactaa
     4621 cgacgtttgg agaagctgta gggcttcctg atggccaaat gggcaactct gaggtaggcc
     4681 acatgaatat cggtgcaggc agagtagtct atcaagaact ggtgcggata aacctagcgt
     4741 tcaaaaacgg tctaatgacc gacaacattg tcctatgcga agcagcagaa tattcattaa
     4801 cacgaggtac atctgttcac ataatggggc ttgtgagtaa tggaggtgta cattcgcata
     4861 tagatcacct tgtaagtctg gtagaaatca tggctgctaa aggagtgaaa aatatataca
     4921 ttcatgcttt cactgacggt cgcgataccg acccacatag tgggttagga tttcttacag
     4981 aagcagaatt aaaaactgca ggtaaagcaa gaatagtaac ggtcattggc cggtattacg
     5041 ccatggatcg agataaaagg tgggagcgaa tcaaagtagc ttatgatgcc ttagtcaatg
     5101 ccaaaggcga gatgacagac gatattttta caaaagtaaa ggaaagttat gcatcagaca
     5161 cctcagatga atttataaga cctattttac taaatacaaa agattacgaa gcatatcata
     5221 ctcgaattaa agatggtgat gtggtaataa actttaattt cagaacagac agaccgagag
     5281 aaataactac agtattgaca caggctgata tgcacgaata caacatgcac aagatggatt
     5341 taaagtatat aagcatgaca gaatatgaca gtacattcaa aaacattgag atactattta
     5401 gaacagaaaa attagaaaag acacttggtg aagtaatagc ggttaacggg ctaagtcaat
     5461 tgaggattgc ggaaacagag aaatatccac atgttacatt cttttttaat ggtggaagag
     5521 aattagcatt tgaaaaagag aacagaagat tgataccatc accaaaagtc gccacttacg
     5581 atatgcagcc ggaaatgagt gctaatggta taaaagaagc agtaattgaa gagataaatc
     5641 acaatcaacc tgatttcatt tgtttaaatt ttgccaatac agatatggta gggcataccg
     5701 gtgtattttt agccgcaatg aaagcagcag aaacggtgga catgtgtctt tcggaaattg
     5761 tacggatagc cctagcaaaa ggctatgacc tttttattat agcagatcat gggaatgcag
     5821 acaacatgat taatgaagac ggtagcgtta acacacagca cagcatgaat ccggttcctg
     5881 taatatttgt atcaaacgaa atcaaatata cgaaaatcaa gcaagggaag ttggcggaca
     5941 ttgctcctac aatattagtc aatatgggag tacaactacc tgaggacatg accggagaga
     6001 atttactaga agaataaata aaactatact gtaaaataga aattcagttt gaaaaataca
     6061 ttaagataat aaaaaacaaa taggcaagga tatattatgt aagaacacaa aaccctatca
     6121 gaatgaactg acagggtatg tgttatatta agttggcag
//
LOCUS       DAOQQY010000172         6139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5487_length_6139_cov_8.042571, whole genome shotgun sequence.
ACCESSION   DAOQQY010000172 DAOQQY010000000
VERSION     DAOQQY010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6139
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5487_length_6139_cov_8.042571"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLE23_08655"
     CDS             complement(<1..995)
                     /locus_tag="PLE23_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMX83372.1"
                     /translation="MIQNISPRQIFLDNIAQTSPFPLQIEIEKASGIYLYDASGKQYM
                     DMIAGISVSCLGHCHPAVTKAVATQAEKYMHTLVYGEFVLSPQTNLASLLASQLPENL
                     QNIYFLNSGTEATEGAMKLAKRYTGRREIISCRNAYHGSTQGSASLMSSEDYTAPFRP
                     LLPGIGHITFNNFDHLEHITEQTACVIMEPVQAEAGIITPEPGYLQAVRERCTQTGTL
                     LIFDEIQTGFGKTGHLFAFLKYGVVPDVLLLGKAMGGGMPLAAFISSKEIMSVLMTNP
                     ILGHITTFGGHPVNCAAGCATLETILSEHLTEQVKYKEDLFLQLLQHPLIKEIRHS"
     gene            1145..1840
                     /locus_tag="PLE23_08660"
     CDS             1145..1840
                     /locus_tag="PLE23_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HMX83373.1"
                     /translation="MRKSCFFFFGMIIISLVSCKEDTVRLMGFPIEGIDVSHHQKDIQ
                     WDSLTDQNFSFVFMKATEGIEHKDTKFDFNWVEAKRIGMKRGAYHFYRPDVGAGQQAV
                     NFIETVTLEEGDLPPVLDFEHKGFKSFESLEDSLRLWLWVVEDYFKVKPIIYTNLNIY
                     NDFIKAHFADYPLWIARYENGGYPISETKSPYIWQYGNRGRISGIDGHVDFNIFLGTQ
                     TDFEKLCYSSKHK"
     gene            1891..2427
                     /locus_tag="PLE23_08665"
     CDS             1891..2427
                     /locus_tag="PLE23_08665"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX83374.1"
                     /translation="MISFKYPVLIVLGVALMVSCKNQEQKPIANETKSEIKQLEDSLL
                     ANQNGKNYLVTAPELIRLMSEYVSKNPTDTVCASYLFKSGDISRGLGKMDDAIKYWQN
                     LCEKYPEHKRAPDALFLQAFTYENDIKDLDKAKKLYEEFLQKYPTHPFAENIPVILAN
                     LGKSPEELIEAFKQKNNN"
     gene            2457..3629
                     /locus_tag="PLE23_08670"
     CDS             2457..3629
                     /locus_tag="PLE23_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMX83375.1"
                     /translation="MSRNILIVMFCLASNMLFAQGIEFFHGSFSEAIEKAKNEEKLIF
                     MDAYASWCGPCKRMASMVFTQSVVGEFYNAKFINMKVDMEKDEGPELSKKFKVSAYPT
                     LIFISPKGEIVQKHVGGMDPDAFVQLGKTALGKADFSADFAKEYENGNRSSDFMLRYI
                     TSMNKSGKSPLKITNDYLATQPDITTSENLKIIHQAAVECDSRIFGLMIKNKSAVIGL
                     VGKDAFNEKVKQAADNTVNKAIEFQSPDLLTEAKAAVKANYPEGYEEFNYTADLKYYK
                     SVGEMENYSKTALEYAQKYLKKDSEQLFKLASDLEAHYFNIPLAMEVAEKIAALSLKN
                     EPKAEYYYLYARLQERNGKVKSAINSCEDGLKIVENGDIVKRKLEDYCQTLKKTIK"
     gene            3633..4787
                     /locus_tag="PLE23_08675"
     CDS             3633..4787
                     /locus_tag="PLE23_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMX83376.1"
                     /translation="MKRSITFIILLHCFNLVQGQGMVFSEGNWQQLLAKAKADNKIIF
                     VDAYTTWCGPCKMMAKNVFTNKEVGDFYNAKFINAQIDMEKGEGMQISRTYSVQAYPT
                     FLFIDGNGTLLHRSVGYQEAPKFLELGRTALDENARIGTMNSRYEDGERDPKFLLKLA
                     YAKYYAMEPDAAEVAEEYLKTQKDWNTPDNLDLIYEFSGGLQSPMTTYMLSNKERFVT
                     RFGQPAMNRKTDFIINNAAATLKGDNPDYEGIRKMLVSLDSASAEQNMLKIKKSHLRR
                     TKNWSEYAVASIDYFNKYPSQNANELNTAAWDFYMYIEHKKQLEEAVRWALQAVKLDN
                     RYYCNDTVAALYTKLGNKSKAKKYIDKAISLAKSEGADYSETEALREKLK"
     gene            complement(4791..5558)
                     /locus_tag="PLE23_08680"
     CDS             complement(4791..5558)
                     /locus_tag="PLE23_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMX83377.1"
                     /translation="MLTIASYNVNGIRAALKKDLAEWILQTDFDILCFQETKVTEDEA
                     PQSELEALGYHSYWHSAEKKGYSGVAVMTKNKPTNVTIGCGMPKYDSEGRIIILDFDG
                     WSLLNCYFPSGSSGEERHEFKMEFLADFRKWIDDLRKTKPNLVIVGDYNIVHGRLDIH
                     NPDRKDNPSGYRPEERTWLDHWFADGFTDAFRHKNPEAKEYSWWSFRAGSRAKNLGWR
                     IDYQSVSDGLKDKIAEAYHLPDAKHSDHCPVVVKYNL"
BASE COUNT         1955 a         1125 c         1312 g         1747 t
ORIGIN      
        1 gaatgccgta tttctttgat gagtggatgc tgtaacagtt gaaggaataa atcttctttg
       61 tacttaacct gctcagtcag gtgttctgag aggatggttt caagtgtggc acatcctgca
      121 gcacaattta ccggatgacc gccaaatgtg gtgatgtgtc cgagtatcgg attggtcatc
      181 aatacagaca tgatctcttt ggaagagata aatgctgcga gaggcattcc tccacccatc
      241 gcttttccaa ggagcagtac gtctgggacg accccatatt tcaaaaaggc aaaaagatgt
      301 ccagttttac caaagccggt ttgtatttcg tcaaagatta gcaaggtacc ggtctgtgtg
      361 catcgttcac ggactgcttg cagataaccg ggctcaggtg tgatgatacc cgcttctgcc
      421 tgtactggtt ccataatgac acatgctgtt tgctcggtaa tgtgctccag gtggtcgaag
      481 ttattgaatg taatatggcc gatgcccggc aatagaggcc tgaacggtgc agtataatcc
      541 tcagaactca tcaggctggc tgaaccttgg gtgcttccat ggtaggcatt tcggcatgag
      601 attatttcac gcctgcctgt gtatcgtttt gcaagcttca tggcaccttc cgttgcttcc
      661 gttccgctgt tgaggaaata tatgttttga agattttcag gaagttgaga tgcaagcaat
      721 gatgcaagat ttgtttgtgg actaagtaca aattcgccat acactaatgt gtgcatatat
      781 ttttctgcct gagtggcaac agccttagtg acagccggat ggcaatgccc aagacaactg
      841 acacttatac cggctatcat gtccatatat tgtttgccgg aagcatcata caaataaatc
      901 cctgaagctt tttctatttc tatctgtagt gggaatgggc tggtctgtgc aatattgtca
      961 aggaatattt gtcttggcga aatattttgt atcattctgt ggccggataa ttgcaaactg
     1021 tgttagaatt gtgttaaata ggcaaaacaa ataccattaa atgtgcatct gatataccta
     1081 cctatttact attaatgtaa aaatacataa atttgttcag taacaaccat acaagctgtt
     1141 aaggatgaga aaatcttgct ttttcttttt cggaatgata ataattagtc ttgtttcttg
     1201 taaagaggat acagtaaggc tgatgggttt tccgattgaa ggtattgatg tttctcacca
     1261 tcagaaagat atacaatggg attcccttac agaccaaaat ttttcctttg tatttatgaa
     1321 ggctactgaa ggcatcgaac acaaagacac aaaatttgac tttaactggg tagaagcaaa
     1381 gcggattggc atgaagcgtg gtgcttatca tttttaccgg cctgatgttg gagccggcca
     1441 gcaagctgtc aattttatag aaacagtgac attagaagaa ggagacctgc caccagtgct
     1501 cgattttgaa cacaaaggct tcaaaagttt cgaaagtctg gaagatagtc tgaggctctg
     1561 gctttgggta gtagaagatt attttaaggt aaaacctatt atttatacaa acctgaacat
     1621 ttacaatgat tttattaaag cccattttgc cgattaccct ctttggatag cccgttatga
     1681 aaacggaggg tatccgataa gtgaaacaaa gagtccgtat atttggcagt atggaaacag
     1741 agggcgtatt tcaggaattg acgggcatgt tgattttaat attttccttg gtacccaaac
     1801 agattttgaa aaactgtgtt attcttcaaa acataaataa aacaacctgg gttttatcaa
     1861 atatagataa ctactcgcaa ttattccact atgatttctt ttaaatatcc tgttctgata
     1921 gtgcttggtg tggcgttgat ggtatcatgt aaaaaccagg agcaaaaacc gatagccaat
     1981 gaaacaaagt cagagataaa acaactggaa gattcgctgc ttgccaacca aaatggaaaa
     2041 aattatctgg taacggctcc agaattaata aggttaatgt cagaatatgt aagtaaaaat
     2101 cctacagata cagtctgtgc aagttatctg ttcaaatcag gagacatatc tcgtggctta
     2161 ggtaaaatgg atgatgcgat aaagtattgg caaaatcttt gcgaaaaata ccctgaacac
     2221 aaacgagcac cggatgcatt gttcctgcag gccttcactt atgaaaatga tataaaagac
     2281 ctggacaaag ctaaaaaact gtatgaagaa ttccttcaga aatatccgac acacccattt
     2341 gccgaaaaca taccggtcat tcttgccaat cttggtaagt cgccagagga gttaatagaa
     2401 gcatttaaac aaaaaaacaa taattagttt attagtataa gttagaattg agcactatgt
     2461 cgagaaatat tttgatagta atgttttgcc tggcaagcaa tatgcttttt gcacagggca
     2521 ttgagttttt tcacggaagt ttttctgaag caatagaaaa agcaaagaat gaagaaaaac
     2581 tcatctttat ggatgcctat gcatcctggt gtggtccttg caagaggatg gcaagtatgg
     2641 tgtttaccca atcggtagta ggcgaatttt acaatgcaaa gttcatcaat atgaaggtgg
     2701 atatggaaaa agatgaaggc ccggaattga gtaaaaaatt taaggtaagc gcttatccaa
     2761 ccttaatatt tatttcacct aaaggagaaa tagtacagaa gcatgtaggc gggatggacc
     2821 ctgacgcatt tgtacaactt ggcaaaaccg cccttggtaa agcagatttc agtgcagatt
     2881 ttgcaaaaga atatgaaaac ggcaaccgta gttctgattt tatgcttagg tatattacat
     2941 ctatgaacaa atccggcaaa tcacctttaa agataaccaa cgattacctt gctactcaac
     3001 cagacatcac aacatccgaa aatctgaaga tcattcatca ggcagctgta gagtgtgaca
     3061 gtaggatatt cggtctgatg atcaaaaaca aatcggctgt tataggtctc gtcggcaaag
     3121 atgcatttaa cgaaaaagta aaacaggcag ctgacaatac agtcaataag gcaatcgagt
     3181 ttcagtcacc tgatttgctt acagaagcca aggcagctgt taaggcaaat taccctgaag
     3241 ggtatgaaga attcaactac acggccgatc tgaagtatta taaaagtgtt ggtgaaatgg
     3301 aaaactacag caagacagca ctggaatatg ctcagaaata cctgaagaaa gattcagaac
     3361 aattattcaa acttgcatct gatctcgaag cacattattt caatattccg ttggctatgg
     3421 aagtagcaga aaagatagca gcactttcac ttaagaatga acccaaggca gaatattatt
     3481 atctgtatgc tagattgcag gaaaggaacg gaaaagttaa atctgcaata aattcttgtg
     3541 aagatggatt aaaaattgta gaaaatggcg atattgtaaa acgaaagcta gaagattatt
     3601 gtcagacact taaaaaaaca attaaataaa acatgaaaag aagcatcaca tttattattt
     3661 tactgcattg ttttaacctt gtgcaaggac agggaatggt attcagtgag ggcaactggc
     3721 agcaattgct tgcaaaggct aaggccgata acaaaatcat atttgtagat gcttatacaa
     3781 catggtgcgg cccatgcaag atgatggcaa aaaatgtttt caccaataaa gaagtaggtg
     3841 atttttataa tgccaaattc atcaatgcac agatagatat ggaaaaaggt gaaggtatgc
     3901 agatatcgag gacatatagt gtacaggcgt atcctacatt tttgtttata gacggaaatg
     3961 gtaccttgct gcacagaagt gtaggatatc aagaagcgcc taaattctta gaactcggaa
     4021 gaacagccct tgatgaaaat gccaggattg gtaccatgaa cagccggtat gaagatgggg
     4081 aaagagatcc aaaattcctc ctgaagctgg cttatgccaa atattacgct atggaacctg
     4141 atgcagccga agtggccgaa gaatacctga aaacccagaa agactggaat acgcctgata
     4201 accttgacct gatttatgaa ttttctggag ggttgcagtc accaatgact acctatatgt
     4261 tgtcgaataa ggaaagattc gttactcgat tcggtcagcc ggctatgaac cgcaaaacgg
     4321 attttattat caataatgca gcagcaacgc tcaaaggtga taatccggac tatgaaggaa
     4381 tacgtaaaat gctggtgtca cttgactcgg cttcggcaga gcaaaatatg ctaaagataa
     4441 aaaagagcca ccttcgtcgt acaaaaaact ggagcgaata cgcagtagct tctattgatt
     4501 atttcaataa atatccttcg caaaatgcaa atgaacttaa tactgcagcc tgggattttt
     4561 atatgtatat cgaacacaaa aaacaactcg aagaagcagt acgatgggca ttgcaggccg
     4621 tgaaactcga taaccgatac tattgcaacg acaccgtagc tgcattatat accaagctcg
     4681 gcaacaagtc aaaagctaaa aaatatatcg acaaagccat ttcactcgcc aagtctgaag
     4741 gtgccgatta ttctgaaaca gaagcgctca gagagaaact gaaataaata ttataagttg
     4801 tattttacga ctaccgggca gtggtcagaa tgtttggcat caggaagatg gtatgcttct
     4861 gcgattttgt ctttcagccc gtctgatacg ctttggtagt ctatccgcca accgaggttt
     4921 ttggcgcgcg agcctgccct gaaactccac cagctgtatt ctttggcttc cgggttttta
     4981 tggcgaaagg catcggtgaa tccgtcagcg aaccagtggt ccaaccaggt gcgttcttca
     5041 gggcggtagc cactcggatt gtctttccgg tcaggattat ggatgtcgag ccggccgtgt
     5101 actatgttgt aatcgcccac gataaccagg ttaggtttgg ttttgcgcag gtcatcaatc
     5161 catttcctga aatcggccag gaactccatc ttaaattcgt gccgttcttc accgcttgac
     5221 cctgacggga aataacagtt caacagagac catccgtcga agtcgagtat gatgatccgg
     5281 ccttcgctgt cgtacttggg cataccacac ccgatggtga cgtttgttgg tttgttttta
     5341 gtcatgaccg ctacgccgga ataacccttt ttctcggcgg agtgccagta agaatgatat
     5401 ccgagagctt caagctccga ttgcggagct tcatcttccg ttacttttgt ttcctgaaaa
     5461 caaaggatgt cgaagtctgt ctgtaggatc cattctgcca gatctttttt cagcgcagca
     5521 cggataccgt taacattata agaagcgatg gtaagcatag tatttttttt gagtggcaat
     5581 attactgttg tttatgttaa aatcctaaac taagtttatg tttcgtcaag agaaacaatg
     5641 gtttgagaaa atgatctctg tttacaatcg ctcttggtaa gaaactgttc gttgggttgc
     5701 gaaacgaagc ttgttcagca gactaggttt ccaaaatata aagccaaatg ttttcaatat
     5761 caataagaga ttcttgactg tttttgtatt tcattcgttt tcgagatact aatcatggat
     5821 ttgcagaaat aattattggc agtaaagaac ctaatgtgtg atttgggttt aacctactga
     5881 ttgaaataat ctttttatag gttttttgga acatttttta aaaccattta cacacagcaa
     5941 ttacatatct gcattgatgt ttacaacggt gagataagca taacaataca tacaaaagaa
     6001 ttatgcgtat tttgaaagat actttcccat aaaattgtgg aaataactcg acaaataatt
     6061 ttttacagat aatcagtaaa gctaaatttg tatctgtgac tatccaacaa aatcaataaa
     6121 tgttttgaag caaattaag
//
LOCUS       DAOQQY010000173         6001 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5695_length_6001_cov_5.270770, whole genome shotgun sequence.
ACCESSION   DAOQQY010000173 DAOQQY010000000
VERSION     DAOQQY010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5695_length_6001_cov_5.270770"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..1446
                     /locus_tag="PLE23_08685"
     CDS             25..1446
                     /locus_tag="PLE23_08685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HMX83378.1"
                     /translation="MLHKERKNAIFYYIIFDYVMAALAWAFFFVFRKLAEGNDFSIAM
                     LDDQNFFRGIILIPLAWVLFFSIFDGYKDLYRLSRLSVFARTFFLSLIGVTFLFFTLI
                     LDDFVKKYTGYYQSFLTLFGLQFGLTVLSRMIILTKASKRLKKGLVTYNTILIGGDQN
                     AVDLYKDVSGLKYGLGNKFIGFIDTNGQSRNELANFLPCLGKIEDISRIIKEFEIEEV
                     IVAIETSEHNRLREILDILFDFGDHVLVKIIPDMYDILLGNVKMNHVFGAVLIEIRQD
                     LMPGWQRIIKRLMDVVLSFLGLLILSPLILYIIIRVKLSSPGPIFYRQERIGQHGIPF
                     YIVKFRSMFTNAESNGPQLSKEGDERCTPWGLIMRKYRLDELPNFYNVLVGEMSLVGP
                     RPERQFFIDQIVKTAPHYKHLLKVRPGITSWGQVKYGYASNIDEMLQRLKFDILYIEN
                     MSLALDFKILFYTVLVILKGSGK"
     gene            1573..2574
                     /locus_tag="PLE23_08690"
     CDS             1573..2574
                     /locus_tag="PLE23_08690"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HMX83379.1"
                     /translation="MKRINLFSIKYLVVALSGMLLLLCTPDVSAQGKGFIHPSDTFSN
                     KRFVLLSGLTGASYAATFAGLGIVWYSQYPKSGFHLFNDAGEWRNMDKYGHAFSGHVT
                     ANLLYNGAKWAGVPEKTAIWSATGVSFGGLLTLEILDGFSAEWGFSVSDLAFNTIGVG
                     AFAVQQALWHDQRIIPKWTSYPTAYPDLVVYSDNGVPMNIRMRAEDLFGTTLAERVAK
                     DYNASIFWLSANIHSFLPNSKVPAWLNVAVGIGAQNMLGGYSNKWIKGGEHFIVPDNV
                     LSRYNQYYIGLDVDLGRIPVRNPYLKTVFGILRHIKIPLPGVEFTSQGKVIFHPVLF"
     gene            complement(2587..3474)
                     /gene="rfbD"
                     /locus_tag="PLE23_08695"
     CDS             complement(2587..3474)
                     /gene="rfbD"
                     /locus_tag="PLE23_08695"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HMX83380.1"
                     /translation="MSQPIIIVTGANGQLGNECLDAASREPQWNITGIGREELDLEHL
                     GDIEEIMDDYKPDIIINCAAYTAVDKAETERKKASLINSEAPAHLAAWCSKNKALLIH
                     ISTDYVFSGASNIPYSENDTTDPVNYYGQSKLQGEKNILEEDKNAVILRTSWVYSEYG
                     HNFVKTMLRLAAENGTVKVVNDQYGSPTYAHDLAEGIFNVIRKYISNDFDKTSVGGTY
                     HFTNSGITNWHDFAKKIFEMSETNCVLTPIPTAAFPTPAKRPEYSSLDTSKYRSAFNW
                     EIPHWEDALRRCLRNMNMS"
     gene            complement(3475..4035)
                     /gene="rfbC"
                     /locus_tag="PLE23_08700"
     CDS             complement(3475..4035)
                     /gene="rfbC"
                     /locus_tag="PLE23_08700"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HMX83381.1"
                     /translation="MPLIKTDFDGLLIFEPKVIEDDRGYFYESYNRRMYEDEGYQYDW
                     VQDNQAFSSRGVVRGLHYQTGDMAQAKLVRVIEGEVLDVVVDIRPGSPTYGKSYSIIL
                     NAENKKQLLVPRGFAHGYAVLSDTAIFFYKCDNHYSKEHEGGILFNDPQLKIDWLLTE
                     SEIQLSEKDKVQPVFNKHRPFFTETK"
     gene            complement(4090..5391)
                     /gene="der"
                     /locus_tag="PLE23_08705"
     CDS             complement(4090..5391)
                     /gene="der"
                     /locus_tag="PLE23_08705"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMX83382.1"
                     /translation="MGSIVAIVGRPNVGKSTLFNRLHGERKAIIDDISGVTRDRIYGV
                     ADWNGKNFTVIDTGGFVHGSDEIFETAIRSQVEIAIEEATAIIFMVDVTTGITDLDEY
                     VAGMLRKTKKSVFLAVNKVDNGQRMLQANEFWSLGFADTFFLSSITGSGTGELLDAVA
                     EVVEDDPLIDPGIPKIAIVGQPNVGKSSLTNALLGESRNIVTDIAGTTRDSIHSEYNK
                     FGKHFILIDTAGIRKKNRVHEDLEFYSVMRAIRAIEEADVCILMIDAVAGVEAQDLNI
                     FGLIKQRNKGVVILVNKWDLVEKETNTARDMEATIKKKIAPFDDVPVIFTSVTEQQRI
                     FKAIEAALEVQENRTRRIKTSVLNEFLEEATTKYAPPSHRGKFNSIKYVSQLPTYYPA
                     FAFFCNHPKYMKDSYRNYLENQLREKFNFKGVPVGIYFREK"
BASE COUNT         1612 a         1176 c         1271 g         1942 t
ORIGIN      
        1 tgataatgtc aaattgatgt attgatgttg cacaaggaac gaaagaatgc tattttctat
       61 tatattatct ttgactatgt catggctgcc ttggcatggg cgttcttttt tgttttcagg
      121 aaactggccg aaggaaatga tttttcaatc gcaatgctcg acgaccagaa ttttttcaga
      181 ggtatcatac ttataccgct ggcatgggtc ttgttttttt ctatatttga cggttacaaa
      241 gatctttacc ggctatctcg actctcggtt tttgccagaa cattcttcct gtcgctcata
      301 ggcgttacat ttttgttttt tacacttatt ctggatgatt ttgtaaagaa atataccggt
      361 tattatcagt cattcctaac cttgttcggt ttgcagttcg gccttacggt tctgtccagg
      421 atgatcattc tcaccaaggc aagcaaaagg ctcaagaaag gcctggtaac atataatacc
      481 atactcatag gcggcgacca gaatgctgta gatctgtaca aggatgtaag cggattgaag
      541 tatggtctgg gcaataaatt cataggattt attgatacca atggacagag tcgcaacgaa
      601 ctggccaatt ttcttccttg tcttggtaaa attgaagata tcagccgcat catcaaagag
      661 tttgagatag aagaagtaat tgttgccatt gaaacctccg aacacaatcg cctgagagaa
      721 attcttgaca ttttgtttga ttttggcgat catgttctag tgaaaatcat accggatatg
      781 tacgatatac tgttaggaaa tgtaaagatg aatcatgtat ttggtgcggt attgatagag
      841 atacggcaag acctgatgcc gggttggcaa cgaatcatca aacgcctcat ggatgttgtg
      901 ctcagtttcc ttgggttgtt gatattgtct ccacttatac tgtacatcat catccgggta
      961 aagttgtctt cacccggacc tatattttac agacaagaac gtattggtca gcatggtatt
     1021 cctttctata tcgttaagtt ccgctcgatg tttaccaatg ctgagtctaa tggcccgcaa
     1081 ttgtcaaaag aaggggacga acgctgtact ccttgggggc ttattatgcg caagtaccgg
     1141 cttgacgaat tacccaattt ttacaatgtt ctggtaggag agatgtcact cgtagggcca
     1201 agacccgaga ggcagttttt tattgaccag atagtaaaga cagcaccaca ctacaagcat
     1261 ctgctcaagg ttaggccggg catcacttca tggggccagg tgaagtacgg ttatgcgtcc
     1321 aatattgatg aaatgcttca aaggctcaaa ttcgatatac tctatattga aaatatgtca
     1381 cttgcactcg atttcaaaat ccttttctat accgttcttg tcatcctgaa aggtagtggc
     1441 aagtaaggat ttaatggaaa ccaatcttgt tattttctaa aaagttaaat ttttatcaat
     1501 aggacatcat ctttctgagg ctttgatgat agtctattta ttacttgcga atattatcct
     1561 aactttgtaa gcatgaagcg aatcaactta ttttcaataa aatacttggt tgtagctttg
     1621 agtgggatgc ttctgttgct atgtacaccc gatgtttccg ctcagggtaa gggattcatt
     1681 catccgtctg atacattcag caacaaacgc tttgtgttgc tgtccgggct gacaggtgcc
     1741 tcttatgccg ctacctttgc agggctcggt attgtctggt attcacaata tccgaagagt
     1801 ggatttcatc ttttcaatga tgccggcgaa tggaggaaca tggacaaata cggccatgct
     1861 ttttcaggcc atgtgacggc aaatctgcta tacaatggtg caaaatgggc tggagtaccg
     1921 gaaaagactg ccatctggtc agccaccgga gtgtcctttg gtggactact tacattggag
     1981 atactggatg gtttttcggc cgaatgggga ttttctgtat cagatcttgc atttaatact
     2041 attggcgtag gcgcatttgc ggtacagcag gcattgtggc acgatcaacg tattataccc
     2101 aaatggacga gttatccgac tgcttaccca gatcttgtgg tttattctga caatggcgta
     2161 cctatgaata tcaggatgag ggcagaagac ttgtttggca caacgctggc agaaagagta
     2221 gcaaaagatt ataatgcatc catcttttgg ctgtcggcta atattcattc atttttacct
     2281 aatagtaaag taccagcatg gcttaatgta gcagtaggta taggggcgca gaatatgctg
     2341 ggtgggtatt cgaataagtg gataaaaggt ggcgagcatt tcattgttcc cgacaatgtg
     2401 ttgtcgcgtt acaatcaata ttacatcggg ctcgatgttg atcttggcag aattcctgtt
     2461 aggaatccgt atttaaaaac agtttttggt attttaagac atattaaaat cccactgcct
     2521 ggcgtagaat ttacttcaca gggcaaggtt atttttcatc cggttttatt ctgaaagaaa
     2581 tttacttcaa gacatattca tattccgaag gcatcttctt agagcgtctt cccagtgggg
     2641 tatttcccag ttgaaggcac tacggtattt gcttgtgtca agagaagaat attcaggtcg
     2701 ttttgccgga gtagggaaag cggcagtcgg tatcggtgtt agtacgcaat tggtttcact
     2761 catttcgaaa atcttttttg cgaaatcatg ccagttggta ataccgctgt tggtaaaatg
     2821 ataggtgccg cctaccgaag ttttatcaaa atcatttgaa atgtattttc taataacatt
     2881 aaatatgcct tctgcaagat cgtgtgcata ggtcggtgag ccgtactgat cgttgaccac
     2941 ttttactgtg ccattttcag cagcaaggcg tagcatggtt ttaacgaaat tgtgaccata
     3001 ctcggagtaa acccaggaag tgcgaagtat aacggcattt ttgtcttctt ccagaatatt
     3061 tttttcgccc tgcagcttcg attgtccgta ataattaacc ggatcagttg tatcgttttc
     3121 ggagtatggt atatttgatg cacccgagaa aacataatct gtcgaaatgt gtataagaag
     3181 ggctttgttt ttgctgcacc atgcagccaa gtgtgccggg gcctcactgt tgattaatga
     3241 tgctttttta cgctctgtct ctgccttgtc aacagcagta taggcagcac agttgatgat
     3301 gatatccggt ttataatcat ccattatttc ttcaatgtcg ccaagatgtt ctagatcgag
     3361 ttcttcgcgg cctattcctg ttatgttcca ttgtggttct ctggatgcag catccaggca
     3421 ttcatttcca agctggccgt tagctccggt gacaataatg ataggctgtg acatttattt
     3481 agtttcggtg aaaaatggcc tgtgtttgtt aaatacaggc tgaaccttat ctttttctga
     3541 taattgaatt tcgctttctg tgagcaacca gtcaatcttg agctgtggat cgttgaagag
     3601 gataccgccc tcgtgttcct tgctgtagtg gttgtcgcat ttgtagaaga aaatggctgt
     3661 atcgctcagc acagcataac cgtgtgcgaa tccgcgtggt accagcagtt gttttttatt
     3721 ttcggcattc aggatgatgc tgtagctttt accgtaggta ggcgacccgg ggcgtatgtc
     3781 cactacaacg tccaatactt caccttctat cacccttacc agttttgcct gtgccatgtc
     3841 acctgtctgg tagtgcaggc ctcgtaccac acctcttgag gagaaagcct gattatcctg
     3901 cacccaatcg tactgatagc cttcatcttc atacattcgt cggttgtagc tctcataaaa
     3961 ataacctcga tcgtcttcta tcacttttgg ttcgaagatc agcaatccgt caaaatctgt
     4021 tttaatcagg ggcattttta caaataatat tggctcaaat atactataaa ttcatgctaa
     4081 aaggcaaaac tacttttcgc ggaaatagat accgacaggc acacctttga aattaaactt
     4141 ctcacggagt tgattttcaa ggtagttgcg gtaactgtcc ttcatgtatt ttggatggtt
     4201 gcagaaaaaa gcaaatgcag gataataagt aggtaactga cttacatact tgattgagtt
     4261 aaattttcct ctgtgcgacg gtggtgcata tttggtcgtc gcttcttcaa gaaactcgtt
     4321 caacacagaa gttttgatac gtcttgttct gttttcctgt acttcaagtg ctgcttctat
     4381 cgccttgaat attcgttgtt gctcggttac tgaggtgaat atgaccggca catcatcgaa
     4441 aggtgctatt tttttcttga tggtagcttc catgtcacgt gccgtattgg tttccttttc
     4501 gacaaggtcc catttgttta caagtatgac cacacctttg ttgcgctgtt tgatgaggcc
     4561 aaatatgttg aggtcctggg cttctacacc tgctacggca tctatcatca gtatgcacac
     4621 atctgcttct tcgatggcac ggatggcacg cattacagag taaaactcca ggtcttcgtg
     4681 tacacggttt ttctttctga tgccggctgt atctattaat ataaaatgtt tcccaaactt
     4741 gttgtattct gaatggatag agtctcgtgt agtgccggct atgtctgtaa cgatgttgcg
     4801 cgattcgccg agcaaggcat tggtgaggga tgattttcct acatttggtt gccctacgat
     4861 ggctattttg ggtatgcccg ggtcaatgag tgggtcgtct tctaccactt ctgctacagc
     4921 atccaggagt tcgccggtgc cgctacctgt gatagatgac agaaaaaacg tatcggcaaa
     4981 tccaagactc cagaattcat tagcctgcag catacgttgg ccattgtcca ctttgttgac
     5041 tgccagaaaa acactctttt tcgtcttgcg aagcattccg gcaacatact cgtcaaggtc
     5101 ggtgataccg gtagtgacat ccaccataaa aataatggca gtagcttctt ctatggctat
     5161 ctctacttgc gagcggatgg cagtttcgaa tatttcatcc gagccatgta cgaacccacc
     5221 tgtatcgatt acagtaaaat tcttgccgtt ccagtcggcc actccgtata ttctgtcacg
     5281 tgtgacacca cttatatcgt caataatggc tttacgctca ccatgcaagc ggttgaacag
     5341 tgttgattta ccgacattcg gccttccgac tattgctaca atacttccca tttagttgaa
     5401 ataagttgca aaagtacaaa aaaatgctga atcaagctgt aaatatataa taatcaggaa
     5461 tttgagtctg aaacccaccg tgtaaggtag gcattctgaa taaatttacc aaatgatacc
     5521 gcgtaaaaag aaaacaggcc gactttagga agtcagcctg ttactacaaa ttataatccc
     5581 atgaacatat ccaaatttcg gagaatcact tttgaaattt tcgtttcgaa ttaacttcag
     5641 ttgtgattac gatacaaata ttcaaactta tctatgcttg aacaaataca aaaatatacc
     5701 tatgtgatat gtaatattat ataaattaat tattataagt agtttaaata tcagataatc
     5761 agcattataa ataatatatc ttattcttat tttataaata atgtgaagaa ttaatttatt
     5821 gttttatgga aaaaaacctg aattttttgt gaaaattttt aaattttttt tcactcattc
     5881 attttttgtc gaaatttttg taaaaataga attggagcag cgcttaaaaa attgatatta
     5941 tatacccaat gtcgtttagt taagcgaaag tatttataaa actttgtaat tcattgattt
     6001 t
//
LOCUS       DAOQQY010000174         5996 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5700_length_5996_cov_5.826124, whole genome shotgun sequence.
ACCESSION   DAOQQY010000174 DAOQQY010000000
VERSION     DAOQQY010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5700_length_5996_cov_5.826124"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..1432)
                     /locus_tag="PLE23_08710"
     CDS             complement(182..1432)
                     /locus_tag="PLE23_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83383.1"
                     /translation="MKTNLVVWGTNANDEKVLILMELMADDNKVVIKTIPENLVSDEL
                     EKKLMDEWRTGSAVELPEGITTIENELSVTDNILPEDLKTDRTDVIHRAQTQWHFIVL
                     SSKLNKLYQNELEDFYENINKLKEYSQETWEDLKNFWQKVQEQVRDKNLLAEHAGNLR
                     ESTNVLFSKLKELKSSLEDETRKASAEILEKFKETMSDIEQKINEGNRLQSIFNDLKE
                     IQNAFRDMKFTKEDRSEAWERLDNAFKSLKDKKYGNNTTNSAEGSPLVRIQRRYDGLK
                     EAVKKMESSIDRDKSELSFHTGKASNTERQLEALVSQAKLAMIQERISSKEEKLAEML
                     KTKEELEARMSELKLKEDARADQEKYEAAKKAAQDKIANEIKQASETRADTNEDSIET
                     EENPVENAIETAIAVSSVEEKDSE"
     gene            complement(1571..2572)
                     /locus_tag="PLE23_08715"
     CDS             complement(1571..2572)
                     /locus_tag="PLE23_08715"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMX83384.1"
                     /translation="MKVAVVGATGMVGQVMLEVLEEFNFPVDEFIPAASARSVGKQVS
                     FKGKNHTIVSLEDAVATKPDIAIFSAGGSVSLEWAPRFAENGTTVIDNSSAWRMDPDK
                     PLIVPEINASVLKKTDKIIANPNCSTIQLVMVLAPLHKKYNIKRLIISTYQSVTGTGM
                     KAVQQFENEKAGIQMTKEEMAYYYPIYNNVIPHCDVFVENDYTKEEMKLVNETKKILN
                     DQSLRITATAVRVPVQGGHSESVNIEFEKPFQTDDVKSILAQTTGVELVDDVKNNQYP
                     MPGFAKNKNEVFVGRIRRDESAENALNLWIVADNLRKGAATNAVQIAQYLLKNNLIG"
     gene            2647..5253
                     /locus_tag="PLE23_08720"
     CDS             2647..5253
                     /locus_tag="PLE23_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMX83385.1"
                     /translation="MKRLLFALVIFLAGNVCHGQILDDFGDGELLTNPEWKGDVANFK
                     VNAALELQLNASDAGSSVIYTPLTIQDSCEWQFYFRLDFAPSNTNLLRVYLQSDSPDV
                     QNAKAYYLEVGETGNLDALRLYRQDGPGAKSLLGTFTLGTLANQPAVCRLKISRNPTG
                     VWQFFADYTGGKNFVKELELTDATYGKGDYYFGFYCLYSATRKDKFYFDDISIKQPVP
                     DTEPPTITSWKVTGQNTFDLTYNEPVDKASAETGSNYIVNNTIGSAVTAVLDQVNTNM
                     VHLSFGNPFQSLQQYTLEVSGVKDLTGNAMQPNQIDFIYTAGKKPLPGDVLFNEIMAD
                     PSPVVGLPEVEFVELYNISTELISVKDLIFTDGSTSASLPDINLLPGGYYILCAQKDT
                     ALLKIFGKTIGVPTLPSINNSGDNLELRSIDGKVIDAVNFSDTWYQDNIKKDGGWTLE
                     LINPLNKCLGKENWTASTSAIGGTPGKQNAVFNPAPDTDPPYITSIFAINADTILVNF
                     NEKTDNNVSQWIDLFTLATKADVVDILSTADRSGVLLVVEPALTEGIYYQLNLAPGFR
                     DCSGNAMVQSQAIEFALPAKPGYHDLVLNEILYNPRTSGYDYLEIYNRTDKVFNLADI
                     TVVNSYGTGNRIKQTANQLIKPKSYIVLSGDPDNIRSNYFVPDSAVIVKASIPAYNDD
                     KGTPGIIYINNGIDVWLDSFYYDKKFHYPLLAAQDGVALERINFNTFESGPSNWHSAA
                     STAGFGTPGYINSQYFEGNTVHGKGIFSLSSKRVSPDNDGYEDFLLVKGEFPQPGFSI
                     SAYVYDAQGRKIKELANNELAGKENTFEWNGLTDEQTLPPLGIYIVFVKYFDLEGNVF
                     EEKLPVVVAGKL"
     gene            5259..>5996
                     /locus_tag="PLE23_08725"
     CDS             5259..>5996
                     /locus_tag="PLE23_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83386.1"
                     /translation="MNLRLIFRNKYVEAITGVFLLLGVLVVILTPDISIIKWTASFAL
                     QTTVLYVLTGLFFLTIAQERLLFVSFFCAASLALFLKGNFNPSLKLPEKSFDSTLNIA
                     YLKLAVVDTNKMKLYDVLKYDPDIICLSKPDSIDTSKLFKSIKGNYPYSVRFRGSSMN
                     QVVFSKFPFITADTILLGGDPQALYSIKFSGYKENVYILNTFLRNSIDGTRSTTYQYD
                     LGRLIVMLRPVNSPLIVVGDFDMVSWSG"
BASE COUNT         1615 a         1202 c         1196 g         1983 t
ORIGIN      
        1 ttacttcaaa tctatttcaa aaactcagaa acccttgttc accatgcttt tatcactttt
       61 atatggaatt ttgtacaatt cattttcctt taaattacga atgcattttt cagggtttaa
      121 taaaaaaagc ctgcataaaa tatattgtgc aggcttttgt ttattaacca tcaaatttct
      181 gttattcaga atctttttct tctacggatg atactgcaat tgcagtttct attgcatttt
      241 ctacaggatt ttcttctgtt tcgatgctat cttcattcgt atcggctcta gtctcggaag
      301 cttgttttat ttcatttgct atcttgtcct gggcagcttt tttggcggcc tcgtattttt
      361 cctgatcagc gcgtgcgtct tcctttagtt ttaattcaga cattcttgct tcaagttctt
      421 ccttggtctt gagcatttct gctaattttt cttcttttga agatattctt tcctgtatca
      481 tggcgagttt tgcctgcgat acaagtgcct caagttggcg ttcggtgttc gaagctttgc
      541 ctgtatggaa cgacaactcc gatttgtctc ggtctattga agattccatc ttttttactg
      601 cctctttcaa tccatcgtat cttctctgaa tgcgtaccaa tggagaacct tcggcagagt
      661 tggtcgtatt attaccgtat tttttatctt tgagtgattt gaatgcgtta tcgaggcgtt
      721 cccaagcttc acttctgtct tcttttgtaa atttcatatc tctgaaggca ttttgaattt
      781 ccttcaaatc attgaatata gattgcaacc tattgccctc attgattttt tgttcaatgt
      841 cagacatcgt ttccttaaat ttttcaagaa tttcggctga tgctttacga gtttcatctt
      901 cgagagaaga ctttagttct tttagcttgg aaaacagcac attggtactt tctctgagat
      961 taccggcatg ctcggcgagc aaatttttgt ctcttacctg ttcttgtact ttttgccaga
     1021 aattcttgag atcttcccag gtttcttggc tgtactcttt gagtttgttg atattttcat
     1081 aaaaatcctc aagctcattt tgatacagtt tattcagttt acttgacaaa acgatgaagt
     1141 gccactgagt ctgagcccgg tgaatgacat cggttcggtc agtcttcaaa tcttcaggta
     1201 ggatgttgtc cgtaacactg agttcgtttt caatagtagt aataccttca ggtagttcaa
     1261 cagccgagcc tgtccgccat tcatccatca gtttcttttc gagttcatca gataccagat
     1321 tttcaggaat tgttttgatg acgactttat tgtcatcagc cattaattcc attaaaatca
     1381 gaactttttc atcgtttgca ttggtgcccc atactaccag gtttgtcttc atacagatgt
     1441 gtactttacg gttttgctaa tttaattcag taatacaaag taaattttgg tccttgtttt
     1501 tgttaagata attatttatt acaaaaagaa tgccttaaat aatacgggca tccgttatgt
     1561 gacaattatt tcatccgatc aaattgtttt tcagaagata ttgtgctatc tgaaccgcat
     1621 tggtggctgc tccttttctg agattatctg ctacaatcca tagattaaga gcattttctg
     1681 ctgattcgtc gcgtctgatt ctccctacaa acacttcgtt tttgtttttt gcaaaacccg
     1741 gcatcggata ctgattattt ttcacatcat ctaccaattc tactccggtc gtttgtgcta
     1801 atatgctttt tacgtcatca gtttgaaaag gtttctcaaa ttcgatattt actgattcgg
     1861 aatgaccgcc ttgtaccggc acgcgtactg cagtagccgt aatacggagc gattggtcat
     1921 tcagaatttt ttttgtttcg ttcaccagtt tcatttcttc tttggtatag tcgttttcga
     1981 caaatacatc gcagtgcgga atgacgttat tataaatcgg ataatagtat gccatttctt
     2041 ctttcgtcat ttgaatgcca gccttttcat tttcaaactg ttggacggct ttcataccag
     2101 tacccgtaac agattgataa gtagaaataa tcagacgctt gatattgtat tttttgtgta
     2161 atggtgccaa gaccatcacc agttggatcg tcgaacagtt cggattggca atgattttat
     2221 cggtcttctt aagcacgctg gcgttaatct ccggcactat caatggcttg tcaggatcca
     2281 tacgccaggc agatgaatta tctatcactg tagtgccgtt ttcggcaaat cgtggagccc
     2341 actcaagtga gaccgaacca ccggcagaga atattgcaat atcaggctta gttgccacgg
     2401 catcttcaag gctgacgatt gtgtgatttt tgcctttaaa gcttacctgt ttgccaaccg
     2461 atctggctga ggcagcaggg ataaattcat ctaccggaaa attgaactct tctagcactt
     2521 ccagcattac ttggccaacc attccggtgg cacctactac agctactttc atgtattcta
     2581 tatgatttat ttaaaataat ggatgtaaag atacagcctt cgtttaaatc ttattaattt
     2641 tgaaatatga aacgtttgct ttttgctctt gttatatttt tggctggaaa tgtttgtcat
     2701 ggtcagatat tggatgattt tggagacggc gagttactca ctaatccgga atggaaaggt
     2761 gatgtggcaa attttaaagt caatgccgcc cttgaacttc agctcaatgc ttctgatgcg
     2821 ggttcttccg ttatatatac gcctctaact atccaggatt cgtgcgaatg gcagttttat
     2881 ttcagactcg attttgcacc gagcaatact aatctgctca gggtttactt gcagtcagac
     2941 agcccggatg tgcaaaacgc aaaagcttac tacctcgaag taggcgaaac cggtaatctt
     3001 gatgcactca ggttataccg gcaggacggc cccggcgcta agtcgctgct cggtactttt
     3061 actttgggta cacttgccaa ccagccagct gtctgcagac tgaagatatc aagaaacccg
     3121 acaggtgtat ggcaattttt tgccgattat acaggaggca aaaattttgt aaaagaactg
     3181 gagctcaccg atgctaccta tgggaaaggc gattattatt ttggttttta ctgcttgtat
     3241 tccgctacac gaaaagataa attttacttt gatgatatat cgatcaaaca accagtaccc
     3301 gatactgagc caccgaccat tacctcatgg aaagtgaccg gccaaaatac atttgacctg
     3361 acatataatg aaccggtcga taaagcaagt gctgagacgg gttcgaatta tattgtcaat
     3421 aatacaattg gcagtgcggt tacagctgtt cttgaccaag taaatactaa tatggttcat
     3481 ctgagcttcg gcaatccgtt tcagtcatta caacaataca ctttggaagt aagcggagtc
     3541 aaggatttga caggtaatgc catgcagccc aaccagattg attttatata tactgcgggc
     3601 aaaaaaccat tgcctggtga cgtactgttt aatgaaataa tggccgaccc ttcaccggta
     3661 gtcggattgc cggaagtaga atttgtagaa ctttataata tttctacaga gcttatttct
     3721 gtcaaagact tgatttttac ggatggttct acatctgctt cattgccgga tattaacctc
     3781 ttgcctggtg gatactacat tttatgtgca caaaaagata cggcattgtt gaagattttt
     3841 ggtaaaacaa ttggagtgcc tacattgcca tctatcaaca actcgggcga caatctggag
     3901 ttgaggagta ttgacggcaa ggtgatagat gcggttaatt tttctgatac ttggtaccaa
     3961 gacaacataa aaaaagacgg aggctggaca ctggagctta ttaatccgct gaacaaatgc
     4021 cttggcaagg agaattggac tgcaagtact tcggctattg gtggcacacc gggcaaacag
     4081 aatgcagtat tcaatccggc acctgataca gacccgcctt acatcacaag tatttttgcc
     4141 atcaatgccg ataccattct ggtaaacttc aacgaaaaga ccgacaataa tgtatcacaa
     4201 tggatcgacc tgtttacact tgccacaaaa gcggatgtag tagatatact gtccactgct
     4261 gatcgttctg gcgttttact agttgtagaa cctgccctta ccgaaggcat ttattaccag
     4321 cttaacctgg ctccaggttt tagagattgt tcgggaaatg ctatggtaca gtcacaagct
     4381 atagaatttg cattgccggc caaacctggt tatcatgacc ttgtgctgaa tgaaatattg
     4441 tacaatccaa ggacttcagg atatgattat cttgaaatat acaaccgcac cgacaaggta
     4501 tttaacttgg ctgatattac ggtagtaaac tcctacggga ccggcaacag aattaagcaa
     4561 actgccaacc agctcattaa gccaaaatcg tacatagtac tcagcggtga tccggacaac
     4621 attcgcagca attattttgt accggacagt gcagtgatag tcaaggcgag cattccggca
     4681 tacaatgacg acaaaggtac acctggtatc atttatataa ataatggtat tgatgtctgg
     4741 ctcgattcat tttactatga caaaaaattt cattatccct tacttgcggc acaagatgga
     4801 gtagcattgg agcgcattaa ttttaacaca tttgaatccg gtccatccaa ctggcatagt
     4861 gctgcatcta cagccggatt tggtactccg ggttacatca attcgcaata ttttgaaggc
     4921 aatactgtgc atggtaaggg tatattcagc ttgtcatcaa aaagggtgtc acctgataat
     4981 gacggatatg aagatttctt actagtgaaa ggcgaatttc cgcagccggg ttttagtatt
     5041 tctgcttatg tgtatgatgc gcagggaaga aaaattaagg agctagccaa taacgaacta
     5101 gccggaaagg agaatacatt tgagtggaac ggacttaccg atgaacagac gctgcctcct
     5161 ttgggtattt atattgtttt tgtaaaatat tttgaccttg agggaaatgt ttttgaagaa
     5221 aaattgcctg tcgtagtggc cggaaaatta taatccgtat gaatctgagg ttgatattca
     5281 ggaataagta tgtggaggct atcactggcg tatttctgct gttaggtgtt ttggttgtta
     5341 tcttaacgcc ggatatttct attattaaat ggactgcgtc atttgcactg caaacaacag
     5401 ttctctatgt acttacagga ttgttttttc ttacaatagc ccaagagcgg ctcttgtttg
     5461 tttcattttt ctgtgcagcc agtcttgctt tatttctgaa gggaaatttt aatccaagcc
     5521 tgaaactgcc tgaaaaatca tttgattcta cactcaacat cgcatacctg aagctcgcgg
     5581 tagtagatac caacaagatg aaactatatg acgtgcttaa atatgacccg gatatcattt
     5641 gcctgagtaa acctgattcg atagatacca gtaagctgtt taaatccatt aagggaaatt
     5701 atccttattc ggtacgtttc agaggctcat ctatgaatca ggttgttttt tcaaagtttc
     5761 cattcattac agccgataca attttgctcg gtggtgaccc acaagctcta tacagtatta
     5821 aattttcggg ttataaggaa aatgtatata tattaaatac ttttctcaga aattcgattg
     5881 acggaacaag aagtacgacc taccaatacg atcttggacg gctgattgtc atgctcagac
     5941 ctgttaattc gcctctaatc gtagtaggag attttgacat ggtttcatgg tcgggc
//
LOCUS       DAOQQY010000175         5995 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5703_length_5995_cov_7.851852, whole genome shotgun sequence.
ACCESSION   DAOQQY010000175 DAOQQY010000000
VERSION     DAOQQY010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5703_length_5995_cov_7.851852"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..1887)
                     /locus_tag="PLE23_08730"
     CDS             complement(46..1887)
                     /locus_tag="PLE23_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83387.1"
                     /translation="MVAVLMNKNYFGIRNNNSKSPVFGNFALNRFTCSSELRPTWDKW
                     NYLSGSWSYAGMAFQGCTVTIGSPAPPFWLPTYSLFDNMNNGIIGLDECILSVRNCRF
                     DTLRHTGLVAVNSHLDFEGIQIGTDLESTVTRCGKYGIRAENSQLSVHKTKFQYNNRM
                     AINSALNSHEEFVKITSNFIQNKSTGISDGIFLQRSSGKAYSTNNIISDNQIIITGEN
                     TNAIGICISGSNNATDNMTISLNDITVNEYPQNGDFLNGRDNHGIMYSAMNHSGYGVF
                     DQNQILNTGLESPTFVLGYGIAFQNNDVFPHTLKNNSTLAMPKPNAFASNDFKKWNIW
                     CGIHADACSNLIYCNNTSEGCFQGMHKLGANAIDDAVISNTFLSNWRGISQDIPAVYG
                     DQECTHNTFLPINNGVEYYHDASFRGATNQLNKFYIDQNDLSQNPDPTTDPNWFAIHS
                     SCENKPAPACPEIFGSRVSGDTEYRIATGELFPDTVDSYDGWAGRYKLYTAIKTYPGV
                     YTDTEYSLFVSQYENSGIGKLYETERLIHEGTVYPDTSKARKLEYDLYIDSCLQSARS
                     LDAQMNITDTVNLSLISLKARLIDQIDSVRLLRADLKSDVMADNGSL"
     gene            complement(1903..2370)
                     /locus_tag="PLE23_08735"
     CDS             complement(1903..2370)
                     /locus_tag="PLE23_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83388.1"
                     /translation="MKTEIINSARILHLDRRFILIFLLLFFGRMISFSQAPTLVLSGL
                     CDKNALESFSTIGNPSVPTLSSSLPSTIFSNQNLEIVGDIIADESISFNNCLLRFSNG
                     KSIFVNKDKVLDIKYSELFSCDKLWKGIVLDNEARVDLFGSYIEDAHTVILTN"
     gene            complement(2620..3837)
                     /locus_tag="PLE23_08740"
     CDS             complement(2620..3837)
                     /locus_tag="PLE23_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HMX83389.1"
                     /translation="MKYFFLLLLTVPHLLHSQTVRIDPEFNRQTADIPGYKLVFADEF
                     NTLDRSVWDLSGPGNDGPSYDAADFLIKSNQVTINPSNVSEPLGSILKIQARKGEDRN
                     MTPFSAGEIKTFNNHSWSNHPPNFRNWTMTPGQYLEARIKIPYCPGINSALWLYGITG
                     AQDDPDRRFFEVDIIESFSEIPSHFWTNIHYGKAYADKSHRSKGREIKVKSLKNKKVV
                     LKDIWLTFGLEYNETDIRMYLNGALYNTYKLYPNSTKKKYRYLKALPAFIRLGVGKAS
                     VESTQAEACQDYPVFLDIDYVRLYTKENLPSIYFLNNYENIWLSATKGRENSGFNMQV
                     NYIPGVKYRVIDEKNQLDITHNENYITACQCAQWWISPKENSPTGPAWIKVMAEFPGG
                     NAINLGTTIQIIP"
     gene            complement(3910..4554)
                     /locus_tag="PLE23_08745"
     CDS             complement(3910..4554)
                     /locus_tag="PLE23_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX83390.1"
                     /translation="MEVISKIPNLSTRASEDYDLVLMAIDGNQKAFAELMDRYRISVY
                     NTMYKMVNNREDAEDLTIEAFGKAFNKLQSYAPHYAFSTWLFKIAINNCIDHIRKRKL
                     QLLSIDEHIQPNSSLEYSHTLKANLLDPEERVIRQQRVNLMRSVLSKLTTKYRLMIEL
                     RFFEELSYEEIAKELDIPLGTVKAQLFRAKELLHNYLQMPGAQDYLEVSSRPRI"
     gene            complement(4545..5582)
                     /locus_tag="PLE23_08750"
     CDS             complement(4545..5582)
                     /locus_tag="PLE23_08750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX83391.1"
                     /translation="MFVKLSHRTPFPEKKCKHPVTTIICARNEAENLNKNLHRILNQN
                     YHLHRLLLVNDNSTDTTPEVLLNIQKNHKTFTLVNAEKTPEGMPGKKAALTQGIENAD
                     TDVLLMTDADCYPASADWAEIMQSQMDEKTEIILGFSPYNKQKGWLNKLIRFDTVMIA
                     MQYFTMALAGMPYMGVGRNMAYRKSLFAKTGGFEKHKSIASGDDDLFIQQAATATNTK
                     ICLDQDSFMISEPKHSWKEFFTQKARHITTGKSYKSHHQIILAMFLITLFVHLISGFG
                     LLSQLMFIRIITAGMILRMTIAWGVFTKIAGLLKSEDLTRWFPLLETSYLLFNIILTP
                     TLLTFKPTKWK"
     gene            complement(5685..>5995)
                     /locus_tag="PLE23_08755"
     CDS             complement(5685..>5995)
                     /locus_tag="PLE23_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83392.1"
                     /translation="EKIAKQHPIYIATTKFVTGFFLYIIYYPLLVKWVLGMGLSGGLS
                     LTIIAGGIICGLLFFPYINILQKLKTSFVTFFVKKNEKEMVKNEILTLQDLISGKNTH
                     "
BASE COUNT         1698 a         1146 c         1192 g         1959 t
ORIGIN      
        1 acgaaatgtt tttgtatttt tgataaaatt tttgaaatat ttttttcaca gactgccgtt
       61 atcagccatg acatcagact tcaggtcagc tctgagaagc ctaactgaat ctatctgatc
      121 aatcaacctt gctttcaggg agataagaga aagattaaca gtatctgtga tgttcatttg
      181 ggcatccaat gaccgggctg attgaagaca ggaatcgata tagagatcat attctaactt
      241 acgtgctttc gatgtgtcag gatatacagt accctcatga ataagacgct ctgtctcata
      301 cagcttgccg atgccggagt tttcgtattg tgaaacaaac aggctgtact ctgtgtctgt
      361 atatacgccc gggtaggtct taatagctgt gtacagcttg taccttccgg cccatccgtc
      421 atagctatca actgtatccg gaaagagctc tcctgtggca atgcggtact ccgtatcacc
      481 cgatacacgc gagccaaata tttcaggaca ggcaggcgca ggtttattct cacatgaact
      541 atgaatggcg aaccaatttg ggtcagtggt cggatctgga ttttgcgata aatcattttg
      601 atctatgtag aatttattta gctgatttgt tgcccctctg aaagaagcat catgataata
      661 ctctacaccg ttgttaatag gcaaaaatgt attatgtgta cattcctgat cgccgtaaac
      721 tgcagggata tcctgagaaa tacctctcca gttagataga aaggtattgc tgataacggc
      781 atcatcaata gcatttgctc ccaatttgtg catcccctgg aagcatcctt cagaagtatt
      841 attacaatat attaaattac tacaagcatc agcatggata ccacaccata tattccattt
      901 tttaaaatca tttgaagcga aagcgttggg cttcggcatt gccaaagtac tgttgttttt
      961 gagcgtgtgg gggaatacgt cattattctg aaaagctatt ccatatccaa gtacaaaggt
     1021 aggtgattcg agtcctgtat ttaatatttg gttctgatca aataccccat atccggaatg
     1081 attcatggcg gagtacataa tgccgtgatt atctcttcca ttcagaaaat caccattttg
     1141 cgggtattca ttaaccgtta tatcatttaa tgaaatagtc atattatctg tcgcattatt
     1201 tgaaccggaa atgcatatac cgatagcatt ggtgttttct cctgttatta ttatctggtt
     1261 atcagaaata atgttatttg tggaataggc tttaccggaa cttcgttgta ggaaaatccc
     1321 atctgatatt cccgttgatt tgttctgaat aaaattagac gtaatcttta caaattcttc
     1381 atggctattg agcgctgaat tgattgccat tctgttgtta tattgaaatt tggttttatg
     1441 tacacttagc tggctgtttt cagccctgat cccatatttt ccacatctgg tgacagtgga
     1501 ttccaaatcc gttcctattt gaatgccctc aaaatccaaa tgactattca cagcaaccaa
     1561 tcctgtatgt ctaagtgtgt cgaacctaca gttcctgact gacagaatac attcatcaag
     1621 acctattatt ccattgttca tattatcaaa taaagaatag gtcggaagcc aaaacggcgg
     1681 tgccggactt ccgattgtca cggtacaacc ctggaatgcc atgccggcat acgaccagga
     1741 cccacttaag tagttccact tatcccatgt gggacgcaac tctgacgaac aggtaaacct
     1801 gtttaaggca aaattaccga aaacaggaga tttgctgtta ttgttcctta taccaaaata
     1861 attcttattc attaatacag ccaccatatt caccacggat ttttaatttg tcagaatcac
     1921 agtatgggca tcttcaatat agcttccaaa taaatccact ctggcttcat tatccaaaac
     1981 aatccctttc cataacttgt cgcaactaaa cagttcacta tatttgatat ccagtacttt
     2041 gtccttattc acaaatatac ttttaccatt actaaatctg agcagacaat tattaaaaga
     2101 aatagattcg tctgcaataa tatcaccgac tatttcaaga ttttgattcg aaaagatagt
     2161 tgatggcaaa gaacttgaaa gagtagggac acttgggttg ccaatagtgg agaatgactc
     2221 gagtgcattt ttgtcacaca aaccagatag taccaaagtt ggtgcctgcg aaaacgaaat
     2281 cattctacca aaaaataata ataaaaagat taaaataaat cttctgtcta aatgtagtat
     2341 tcttgcagag tttattatct cggttttcat aacaataaat taaaataaat aaaaccgaaa
     2401 gataaagtat aaatgataaa aaagcaagtt ttctgctgca tgttttcatt aaaaaataaa
     2461 cagcataaaa atcaatatat ttcggaataa attaaaaata ataaaggtga aaccgaattt
     2521 aatttgtctg taacagtaac ttaaattcat ctgatattca tgcttgtttg actgccaatt
     2581 caattattta atttgttata tagcagttcc ttggctaatt taaggaataa tttgaatggt
     2641 ggtacccaga tttattgcat ttcctccggg aaactccgcc ataactttaa tccaggcggg
     2701 acctgtcgga ctgttctctt tcgggcttat ccaccattga gcgcactgac aggcagtgat
     2761 gtagttttca ttgtgcgtaa tgtcgagctg gtttttttcg tctataacac ggtacttaac
     2821 acccggaatg tagttgacct gcatgttgaa tccgctgttt tctctgccct tggttgctga
     2881 gagccagatg ttttcatagt tatttaaaaa ataaatggac ggcagatttt ctttggtata
     2941 tagccggaca taatcaatat ctaagaatac cggataatcc tggcatgctt ccgcttgggt
     3001 tgattctacg gaggccttgc ctacacccag tctgatgaag gcaggcaaag ccttcagata
     3061 gcggtatttt ttctttgttg agttaggata taatttgtag gtattataaa gtgcaccatt
     3121 cagatacatc ctgatgtctg tttcattgta ttcgagtccg aatgtgagcc aaatatcctt
     3181 gagtacgact ttcttgtttt ttagactttt tactttgatt tcccgacctt tactgcggtg
     3241 cgatttatct gcgtatgctt tgccgtaatg tatgttggtc cagaaatgag acggaatttc
     3301 gctaaatgat tcaatgatat ctacttcgaa aaagcgcctg tcgggatcat cctgagcacc
     3361 ggtaatgcca tatagccaca gcgccgaatt gataccgggg cagtatggta ttttgattct
     3421 ggcttcgaga tattgacccg gagtcatggt ccagtttctg aaattgggag gatgattact
     3481 ccaactgtgg ttattgaagg tcttaatttc gcctgcagag aatggggtca tatttcggtc
     3541 ttcgcctttt cgagcctgta ttttcaggat actgcctagt ggttcagaca cattagaagg
     3601 attgatggtt acttggttgc ttttaatcaa aaaatcagcc gcatcatagg acggaccatc
     3661 attgcccgga ccagacaaat cccaaacaga acggtcaagc gtattaaatt cgtcagcaaa
     3721 cactaattta tatcccggga tgtcagcagt ctgccggttg aactccggat ctatcctcac
     3781 agtctgtgaa tgaagcaaat gtggtacagt caagagcaaa agaaaaaagt atttcatgtt
     3841 tgcttagatt ttacaagaaa caaaaaaacc ccgttgccga ggtttttctg tatttagttg
     3901 atatgttcat tatattcttg gtcggcttga tacttcgaga taatcctgtg cacctggcat
     3961 ttggagataa ttatggagca attccttggc tctgaatagt tgtgctttga cagtgccgag
     4021 tggaatgtcg agctcttttg ctatttcttc gtagctcaat tcttcgaaga agcggagttc
     4081 gatcatcagc ctatatttgg tagtcagttt gctcaatacg cttctcataa ggtttaccct
     4141 ttgctggcga atgactcttt cttcggggtc aagcaggttg gctttcagcg tatggctata
     4201 ctctagcgaa ctgtttggct ggatgtgttc gtcgatagac agtagctgaa gttttctttt
     4261 tcggatgtgg tcgatacagt tgttgatggc aattttgaac aaccacgtac tgaacgcata
     4321 gtgcggagca tagctctgga gtttgttaaa tgctttgcca aatgcttcga tggtcaggtc
     4381 ttctgcatct tcacggttgt taaccatttt gtacatggtg ttataaactg agattctgta
     4441 acggtccatg agttctgcga aagctttttg gttgccgtca atggccatta gtacaagatc
     4501 gtagtcttca ctcgctctgg tagaaaggtt tgggatttta ctgattactt ccattttgta
     4561 ggtttaaagg tcaataatgt tggtgtaagt ataatattaa ataacaaata actggtttcg
     4621 agcagtggga accatcttgt cagatcctca cttttgagta atccggcaat ttttgtgaag
     4681 actccccaag ctattgtcat tctcagaatc atgccagccg taatgatacg gatgaacatc
     4741 aactgtgaca ataacccgaa cccgctgatc aaatgaacaa aaagtgttat caaaaacatc
     4801 gccaaaatga tctggtggtg acttttatag ctttttccgg ttgtaatatg ccttgctttt
     4861 tgagtaaaaa actctttcca gctgtgttta ggttcagaaa tcatgaatga atcctggtcc
     4921 aggcatattt tggtgttagt agcggtagct gcttgttgta tgaaaagatc atcatcacct
     4981 gaggctatac ttttgtgttt ttcaaagccc ccggtcttgg cgaacagcga ttttcggtaa
     5041 gccatatttc taccgacgcc catgtatggc atgcctgcca gggccatcgt gaaatattgc
     5101 attgcaatca ttactgtatc aaacctgatt aatttgttca gccagccttt ctgcttgttg
     5161 tatggcgaaa aaccaagaat tatctcagtt ttttcatcca tttgtgactg cataatttca
     5221 gcccagtcag cagaggcggg gtaacaatca gcatcagtca ttaaaagaac gtctgtatct
     5281 gcattttcta ttccctgagt gagcgccgct ttttttcctg gcataccttc aggtgttttt
     5341 tctgcattta caagtgtaaa ggtcttatga tttttttgaa tattcaatag cacttcgggc
     5401 gttgtgtcgg ttgaattatc gtttaccaat aagagcctgt gtaagtggta gttttgattt
     5461 aggattcggt gcaagttttt attaaggttt tcggcttcat ttcttgcaca tataattgtt
     5521 gttaccgggt gcttgcattt tttttcagga aatggtgttc tgtgtgacag ttttacaaac
     5581 ataataatcc agtacgctat ctgaattaat gttgatatga taaataaacc caacacgatt
     5641 tttgggatca tggaattagt atgtttgtga aatcaatgcg caatttagtg ggtattttta
     5701 cccgaaatca agtcctgaag tgttaaaatt tcatttttta ccatttcttt ttcatttttc
     5761 ttcacaaaaa atgtaacaaa agatgtttta agcttctgta atatgttgat gtacgggaaa
     5821 aaaagcaagc cgcagatgat gcctccggca ataatagtca agcttagtcc gcctgataaa
     5881 cccataccca atacccattt caccaataaa gggtaataaa tgatgtagag aaagaatccg
     5941 gtaacgaatt tggtagtagc aatatatatt gggtgttgtt tggcaatctt ttcaa
//
LOCUS       DAOQQY010000176         5970 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5748_length_5970_cov_5.238546, whole genome shotgun sequence.
ACCESSION   DAOQQY010000176 DAOQQY010000000
VERSION     DAOQQY010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5748_length_5970_cov_5.238546"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PLE23_08760"
     CDS             <1..146
                     /locus_tag="PLE23_08760"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HMX83393.1"
                     /translation="TCRPFDKAGAYGVQDWIGLCKIESITGTYSNIMGLPVNLVYEQL
                     KKF"
     gene            complement(156..701)
                     /locus_tag="PLE23_08765"
     CDS             complement(156..701)
                     /locus_tag="PLE23_08765"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMX83394.1"
                     /translation="MRDLEPRKVNDSRSVMTEMVMPNDTNPLGNLMGGNLMRWMDIVA
                     SICAGKHCEAHVVTASVDHVSFQKPIDLGDVVTLQACVTRAFNTSVEVSVEVFAADIQ
                     GKNNRMCNHAYFTFVGLDYKSRKPIPIPGVIPLTREEQAMYDGALRRRQLRLILSGRL
                     SPDDATELKSLFIKHDATVSK"
     gene            complement(707..1105)
                     /locus_tag="PLE23_08770"
     CDS             complement(707..1105)
                     /locus_tag="PLE23_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83395.1"
                     /translation="MKKDIPVQKVTDVIVAIVPREEEDEFWDVYLINNKEFPILSVII
                     NSTGYGEIEGEKVKTSTMRYFYDEVPARHAVKIEPILKNLFSITNEFWVSFSLGNYLY
                     DKKYIFVEGSLNEINFTGVPIVGKQGVMIK"
     gene            complement(1102..1854)
                     /gene="lipB"
                     /locus_tag="PLE23_08775"
     CDS             complement(1102..1854)
                     /gene="lipB"
                     /locus_tag="PLE23_08775"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HMX83396.1"
                     /translation="MTDNHLNTNKLLKDCVVYDWGKIQYKEAWDKQTILANELIAAKK
                     QSDFPVNEHPYKLIFCEHNPVYTLGKSGSIEHIKLSDKELSERGIEFYKINRGGDITY
                     HGPGQLVCYPIFDLERISRDLKRYISSLEEVVIRTLAAYEIFGIRIPEYTGVWLEATE
                     VLPLRKICAIGVHMSRWVSMHGFAFNINTDLAYFRNIIPCGINDSNKAVTSLAIELGK
                     EIDMNHVKKQLLFHFSEVFGLKYAKEDAKILL"
     gene            complement(1851..3074)
                     /locus_tag="PLE23_08780"
     CDS             complement(1851..3074)
                     /locus_tag="PLE23_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HMX83397.1"
                     /translation="MNTKIEEVLKVVKKAVQLLPNPPRDSINNEIQILQELIMQSRPP
                     KIMIIGRRGAGKSSLINAFFNEEVAAVGSVSSKTPFGKWYSYTSESGSIDIMDTRGLG
                     DSSRPEDSKFATAMEELMAELSEKCPDVVLFLSKAKEVDSRIDEDIDNVKQILAFIKK
                     KHNYEPPVIAAITQVDELDPVYIDKPPYEHETKQKNIAEATARIEQLFVDAKIAILKV
                     VPVSAYAVFEYGKMVYNRFWNIDALIDFLVDSLPREAKLELARLAKLVDVQKKIARII
                     ITSAATINAGIAAIPIPVADIFPITSTQIAMIAGIGYITNRKLDKKTIIEFLSAIGAT
                     TGAAMAFRELARGIIKFVFPGGGSVISSSIAFGGTWAIGEAAIAYFIEGKSISDVKEV
                     FDTIKKKKEKEFEKK"
     gene            complement(3219..4301)
                     /locus_tag="PLE23_08785"
     CDS             complement(3219..4301)
                     /locus_tag="PLE23_08785"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMX83398.1"
                     /translation="MSIENQLSDLLRLTGHTVIPGIGRFQLEYRPAVIDYIQKHISTP
                     GKFVNFHFNESLLINDIPDAQCAEIEKSLWESAGINWQERLNNKETILLPNVGSVYKD
                     FRGEIHFIPSNVNYDDASYGLRQLNIEPVIRKSEVAPVAPVVPKPVSRPKSNFQFADT
                     LFSVLIGFTLIAISFGVWYAMSSKQISQPASRFPIAESTLEDQKNIEDILDYDAKWSV
                     PGYYGEDSVASDSVSEDVASNDDQDTAVLNEVEVPKPAVPVASGKPKTNPAIKETNIR
                     MRNAVKATPTDLSCMISVGSFSNDANAKSLLKKLSAKGYHTTIKINETNNMSRVVVMC
                     TCDEAVLNATLARLKDGYNAGAYIVQ"
     gene            4622..5659
                     /locus_tag="PLE23_08790"
     CDS             4622..5659
                     /locus_tag="PLE23_08790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMX83399.1"
                     /translation="MNRNPENGHRLRLISYVVIIYLLLAFSWWAILLYVKNNDAFYAK
                     AAYMELIQKGTQRVHTSEEFYLTSEYTELKRKYEKQELMIFGEAMVFVLSLIAGIFFI
                     NRGYYREVQLARQKKNFLMSVTHELKSPLASIRLVLETIQKRDLTDEQKKQLTESAIK
                     ENDRLTSVVHNLLTATRLDTMYQANLELMSLSKVVKDEISRMKMIFPERRITLDCEEN
                     LPELYFDRAGMISVISNLVENAIKYSSITDEVRVTLSGAHDCLQLRVFDNGTGVAPEE
                     KFKIFEQFYRSGNEETRNTKGTGLGLYIVKQIVLAHQAKITLRENTPKGSIFEVSLPL
                     KIKNGPKDSIG"
     gene            5634..>5970
                     /locus_tag="PLE23_08795"
     CDS             5634..>5970
                     /locus_tag="PLE23_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMX83400.1"
                     /translation="MAQRILLVEDEESILTTLKLNLEFEGYEVVYATDGKTALDLFKS
                     QYFHLVVLDIMIPILSGLDVCEQIRLTDSETPIIFLTARDTIPNKIDGLKKGADDYLM
                     KPFTADELIA"
BASE COUNT         1746 a         1275 c         1099 g         1850 t
ORIGIN      
        1 ctacctgccg accattcgat aaagccgggg catacggtgt gcaggattgg attggtctat
       61 gtaaaataga atccattaca ggtacgtact ccaatattat gggactgcct gtcaacctcg
      121 tttacgaaca gctcaaaaaa ttctgaccta caaaattatt ttgacacagt tgcatcgtgt
      181 ttgataaaca atgacttgag ttctgtagca tcatccggag acaatctgcc actcaatatc
      241 aatcgtagtt gtctccttcg gagtgcgcca tcatacatag cttgttcttc tctagtaagt
      301 ggaatgacac ccggtatagg tataggttta cgcgatttat agtcaaggcc tacgaaagta
      361 aaatatgcgt ggttacacat acggttgttc ttaccctgga tatcagctgc aaatacctca
      421 accgaaactt ccaccgatgt gttaaaagct cttgtaacac acgcttgtag tgttactaca
      481 tcacccagat caataggttt ttgaaacgat acgtggtcaa ctgaagctgt tactacatgt
      541 gcttcacaat gctttccggc gcaaatactg gctacaatat ccatccatcg catcaggttg
      601 cctcccatta gattgccaag tggattggtg tcattgggca ttaccatttc cgtcatcacc
      661 gaccttgagt catttacttt gcgcggttct aaatctctca tttctattat tttatcatta
      721 caccttgttt accaacaatc ggtactcccg taaagtttat ctcattgagt gaaccttcca
      781 caaaaatata ctttttatcg taaaggtaat tgcctaaaga aaaactcacc caaaattcat
      841 tggtaattga gaacagattc ttaagtattg gttcaatttt gacggcgtgt ctggcgggca
      901 cttcatcata gaaatatctc atagtagatg tcttcacttt ctctccttct atttctccgt
      961 atccggtcga attaataatt acagacagaa tcggaaactc tttattgttt ataaggtaca
     1021 cgtcccaaaa ctcatcttcc tcttcacgtg gtacaatggc aacgattaca tctgttactt
     1081 tctgaacagg aatatctttt ttcataaaag tatttttgca tcttctttag catatttcaa
     1141 gccaaagacc tcagaaaaat gaaataacaa ctgtttttta acatggttca tatcaatttc
     1201 ttttcctaat tctattgcta agctggttac ggctttgttg ctatcattta ttccacatgg
     1261 tatgatattt ctgaaatacg ctaagtctgt attaatatta aatgcgaacc catgcatact
     1321 tacccaccta ctcatgtgta ctccgatggc gcatatcttc ctaagtggca gcacctcggt
     1381 agcctcaagc cagacaccgg tatattcggg tatccggatg ccaaatattt cgtatgctgc
     1441 gagtgttctg atgacgactt cctccagact tgaaatgtat cttttcagat cgcgacttat
     1501 acgctccaag tcaaaaattg gataacaaac caactgaccg ggcccatgat atgttatatc
     1561 gccaccacga tttattttgt aaaattcaat gcccctttca gacagttcct tatcactgag
     1621 tttgatatgt tctatagagc ctgatttgcc aagcgtatag accggattgt gctcacaaaa
     1681 tatcaattta taaggatgtt cattgacagg aaaatcgctt tgcttttttg cagcaataag
     1741 ttcattggcc agaatggttt gtttgtccca agcctccttg tactgtatct tcccccagtc
     1801 ataaactaca caatctttaa gaagcttatt tgtattcaaa tggttatctg tcattttttc
     1861 tcaaactcct tttctttttt ctttttgatc gtgtcaaaaa cttctttgac atcactgatt
     1921 gattttcctt caataaagta ggcaatagca gcctcgccta ttgcccaagt accaccaaat
     1981 gctatggatg acgaaataac cgagcctcca cccggaaaaa caaacttgat gataccacgt
     2041 gcaagctcgc gaaatgccat agccgctcct gtagtggcac ctattgccga cagaaattcg
     2101 atgatagttt ttttatcaag cttacggttg gtgatatacc caatacctgc aatcatggct
     2161 atctgcgtgg atgttatagg aaaaatatct gctaccggaa tcgggatggc tgctatgcca
     2221 gcattgatgg tggcagctga agtgatgata atgcgtgcta tcttcttctg tacgtccacc
     2281 aacttggcca gccttgccag ttcgagtttg gcctcacgtg gcagactatc taccagaaag
     2341 tcaatcagtg catctatatt ccagaatcgg ttataaacca ttttcccata ctcgaacact
     2401 gcataggcag acaccggtac gacttttaat atggcaattt tagcatcgac gaatagctgt
     2461 tcaattctcg ctgtggcctc agctatattc ttttgcttgg tttcatgctc ataaggcggc
     2521 ttatcgatat aaaccgggtc cagctcatcc acttgtgtaa tagcagctat cacaggtggt
     2581 tcataattat gtttcttctt aatgaatgca agtatctgct tcacattgtc tatgtcttca
     2641 tcaatccggc tatccacctc ttttgccttt gacagaaaca acactacatc cggacatttt
     2701 tcgctcagtt ctgccatcag ttcttccata gcggttgcaa acttcgagtc ttcgggcctc
     2761 gagctgtctc ccagtcctcg ggtatccata atatcgatac tgcctgattc agaagtgtaa
     2821 gaataccatt tgccgaaagg cgtcttacta ctcactgaac caactgctgc tacttcttca
     2881 ttgaaaaaag cattaataag cgaagattta ccggctccac gacggccaat aatcattatt
     2941 tttggaggcc tgctctgcat gatcaattct tgtagaatct gtatttcgtt attaattgaa
     3001 tccctcggcg gattcggtag taactgtacc gcctttttca ccactttcaa cacttcttct
     3061 atcttggtgt tcatgttttg taataaatta tgaattcaat attgagagat ttttttgtct
     3121 ttgaggtatt tttctttggt taaattggaa gtaatgctta ttgaataaat cacagttcga
     3181 aagctaaact atggttttcg tcaggttcta ttgcaaaatt attgaacgat gtaagctcct
     3241 gcattatagc catccttcag tctggcaagc gtggcattca gtacagcttc atcacaagta
     3301 cacatcacca ctacccgaga catattgttc gtttcgttga ttttgatggt tgtatggtat
     3361 cccttagcac tgagtttctt caataatgat tttgcattag catcatttga aaatgagcct
     3421 acactgatca tgcatgacaa atctgtcgga gtggccttca cagcattgcg cattcttatg
     3481 tttgtttcct tgattgctgg gtttgtttta ggttttcctg aagctactgg tacagccggt
     3541 ttgggtactt ctacttcgtt tagtacggca gtatcttgat cgtcatttga tgcaacatcc
     3601 tcgcttactg aatctgatgc gactgaatct tcaccataat aacccggcac agaccacttt
     3661 gcatcgtaat caagtatatc ttctatattt ttctgatctt caagcgttga ttctgctatc
     3721 ggaaacctag atgcaggctg tgaaatctgc ttggagctca tagcatacca gactccaaaa
     3781 ctgatggcaa tgagtgtaaa tccgatcaaa actgaaaata acgtatccgc aaactggaaa
     3841 ttggatttcg gtcgcgatac cggtttgggt actaccggag caacaggtgc tacttccgac
     3901 ttacggatta ccggctcgat gttcagctga cgaagaccat agcttgcatc atcgtaattg
     3961 acattagaag gaataaaatg tatttcacct ctaaaatctt tataaaccga tccaacattt
     4021 ggtaacagaa tggtttcttt attattaagt ctttcctgcc agtttattcc tgctgattcc
     4081 caaagagatt tttctatttc ggcacattgc gcatcaggta tatcattgat aagcaacgat
     4141 tcgttaaaat gaaaatttac aaatttgccc ggtgtggaaa tatgtttttg aatataatca
     4201 atgactgcgg gtctgtattc aagctgaaac cgcccgatgc caggtatcac tgtgtggcct
     4261 gtaagcctta acaaatcgga caattggttt tcaatagaca tttgattgtt gtgtatgtaa
     4321 gttctggttg caaatgtaaa aataaaaaat aataatttat ataaacacct ttctttcaat
     4381 aagcaaaatg tcattttatt acaatgtact aattttgtgc catattggat gaattgataa
     4441 tgaaccacat acccgaagat gcatcgttac tgcaattttt acaaaataat gaaccacatc
     4501 acgtacttct ttttaacaaa atacattact ttgtagggag aaatacatca attaacattt
     4561 tttcaaacat gaatcactaa ctatgatgat ctaaggaatc accttttatc ccttaaataa
     4621 tatgaaccga aatccagaaa acggccaccg gttaaggctc atttcttatg ttgttattat
     4681 ctatctgttg ctggcattca gttggtgggc gatattgctt tacgtcaaaa acaacgatgc
     4741 attctatgcc aaagctgcct acatggagct catacagaaa ggcacacaac gcgtacacac
     4801 gtctgaagaa ttttacctga caagtgaata tactgaacta aaacgcaaat acgaaaaaca
     4861 agagctcatg atatttggtg aggcaatggt gtttgtgtta agtcttatag caggtatatt
     4921 ttttataaac cgtggttact accgcgaagt acagcttgcg agacaaaaga aaaatttcct
     4981 tatgtcagta acccatgagc ttaagtcgcc ccttgcctcc atccgtcttg tgctagaaac
     5041 catccaaaag cgcgacctca cagatgagca gaaaaaacaa ctaaccgaaa gtgctatcaa
     5101 agaaaatgac cgcctgacaa gtgtagtaca caacctactg acagccacaa gattagatac
     5161 aatgtaccaa gctaatcttg aacttatgag cctgtcaaaa gtagtaaaag atgaaatctc
     5221 tcgtatgaaa atgattttcc ctgaaagaag gataacactg gattgtgaag aaaatttgcc
     5281 cgaattatat tttgacagag ccggcatgat atccgttatc agcaatcttg tggaaaacgc
     5341 tatcaaatat tcctccatca cagatgaggt aagggttaca ttgtcgggtg cacatgactg
     5401 cttacaatta cgtgtatttg ataatggaac aggagtagca cctgaagaaa aatttaagat
     5461 attcgaacag ttttatcgca gtggaaatga ggagacgcgc aacaccaaag gtaccggatt
     5521 gggactttat atcgtcaaac agatagttct agctcatcaa gctaagataa cattacgtga
     5581 aaatacaccc aaaggtagta tctttgaggt ttctttacca ttaaaaataa aaaatggccc
     5641 aaaggattct attggttgaa gacgaagaaa gcatacttac cacactcaaa ttaaatctgg
     5701 aatttgaagg ttacgaagtg gtatatgcca cagatggcaa aacagcactc gatttattca
     5761 aaagccagta tttccatctg gtcgttcttg atataatgat accgatactg agcgggctgg
     5821 atgtatgcga gcaaataagg ctgacagact ccgaaacgcc catcattttc ctaactgccc
     5881 gtgacaccat tcctaacaaa atagacggcc ttaaaaaagg agcggacgat tatctcatga
     5941 agccatttac ggcggacgaa ctgatcgctg
//
LOCUS       DAOQQY010000177         5954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5768_length_5954_cov_5.860315, whole genome shotgun sequence.
ACCESSION   DAOQQY010000177 DAOQQY010000000
VERSION     DAOQQY010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5954
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5768_length_5954_cov_5.860315"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /gene="msrB"
                     /locus_tag="PLE23_08800"
     CDS             complement(<1..456)
                     /gene="msrB"
                     /locus_tag="PLE23_08800"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMX83401.1"
                     /translation="MNAMIYLACILVSFVNACTFNLRSTPETAKPATKLVSEVENDTI
                     KPIVKTEAEWKKELSPMCYNVMRESGTERAFTGKYWDNHAKGTYYCAACRLPLFESDT
                     KFESGTGWPSFFDVKSKSNVKLVTDIDYGMERVEVRCARRTEALGQRLGL"
     gene            complement(470..910)
                     /locus_tag="PLE23_08805"
     CDS             complement(470..910)
                     /locus_tag="PLE23_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83402.1"
                     /translation="MVAYNLDNPRKLATTSLIGSLILSFGFFLLVSAGMVEPYPRFYW
                     MSACTFLMLYAIFNSVISTRVENNPNYWNTSVVVYLILAVVTGVVAWAMSDLSVFNAG
                     SYSFLYVVITFGYMVFISVMKAVKSLIEYAQKEVWHHPKMRKRK"
     gene            complement(917..2233)
                     /gene="rimO"
                     /locus_tag="PLE23_08810"
     CDS             complement(917..2233)
                     /gene="rimO"
                     /locus_tag="PLE23_08810"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HMX83403.1"
                     /translation="MKTKTLRKDIVNVITLGCSKNLVDSENLITQLKGNDFAVTHEDT
                     TSDANVVIINTCGFIDLAKQESINTILEYAAKKSDGEIEKLYVTGCLSQRYKDALEEE
                     IPEVDAYFGTLELPGLLAKLNADYKHELLGERLITTPQHYAFLKISEGCNRTCSFCAI
                     PLMRGGHISRPIEELVAECKNLARHGVKEIMLIAQELTYYGLDLYKKRELARLLEALC
                     DVEGIEWIRLHYAYPSKFPLEVLDVMAAQPKVCNYLDLPVQHANDEVLKRMKRQITQQ
                     ETRELIEHARRIVPDISIRTTLIVGFPGETEEEFQDLCTFVQEMEFDRLGVFQYSHEE
                     DTFAHQMEDDVPAPIKEARAARIMEIQKEISFDKNQGKIGKTFKVLIDKKEGDYFVGR
                     TESDSPEVDNEVLLSAKENYARIGDFVHVRIDDANEFDLFATIVKN"
     gene            2325..2975
                     /locus_tag="PLE23_08815"
     CDS             2325..2975
                     /locus_tag="PLE23_08815"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HMX83404.1"
                     /translation="MKNLLAAIIFLCFCSNMHAQIGFQVQYQRFQDTSYILSGSGFNE
                     FVQADQLGFGIDYAFKLKNYRVEFFPAILYFNKKINKNSIFDTELNTNITTLGLQFNT
                     HIYFLDIEGDCNCPTWNKDGSFMKKGIFAWVSINAYNTVFKQNTPATRNMFTYGASGG
                     LGLDIGLSKHLTLTPFAGLELEQRPVVEFTDLKAERDTKSMYKLDAGIRLGWHWGK"
     gene            complement(2982..3515)
                     /locus_tag="PLE23_08820"
     CDS             complement(2982..3515)
                     /locus_tag="PLE23_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HMX83405.1"
                     /translation="MRKLSTSELDRISADKVNTIRKMPLVVVLDNIRSGLNVGSVFRT
                     CDAFAVSHIFITGFTPQPPHREILKTALGSTETVSWTYVESTEDCLKLLEKEGFYNIA
                     IEQTTESISLEKFVFHHDRKYALILGNEVDGVSDEAIAIADAAIEIPQAGVKHSLNVA
                     VCGGMVLWEAFRQLYLP"
     gene            complement(3518..5137)
                     /locus_tag="PLE23_08825"
     CDS             complement(3518..5137)
                     /locus_tag="PLE23_08825"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMX83406.1"
                     /translation="MTKYVFVTGGVTSSLGKGIIAASLAKLLQSRGYKVTIQKFDPYL
                     NVDPGTLNPYEHGECFVTDDGAETDLDLGHYERFLNIPTSQANNVTTGRIYRSVIDKE
                     RRGDYLGKTVQVVPHITNEIKRRVKLLGQDKKYDIVITELGGTVGDIESLPYLEALRQ
                     LRWELGPDNCITIHLTLIPYLRAAKELKTKPTQHSVKELLRSGVQPDILVCRTEHALP
                     KELRKKVALFCNVETEAVIEAMDAETIYSVPVLMAKENLDTIVLEKLKLTSPKELDLS
                     AWNSFLDKLKNPTSKVQIGLIGKYNELQDAYKSIYEAFIHAGASNLCKVNVIPIHAEK
                     LIDSEKEIEKLNKLDGILVSPGFGERGLLGKLVAIKKCREEKIPFFGICLGMQCAVVE
                     FARNAAGLTNANTTEADPNTEYPVIDMMESQKALTQKGGTMRLGSYKCELLKNSKAFK
                     AYGQTKINERHRHRFEFNNQYINILEENGLKATGHNPETGLVEIVEIPDHPWFVGVQY
                     HPELKSTVEKPHPLFVAFVKAAIDYKESQGK"
     gene            complement(5214..>5954)
                     /locus_tag="PLE23_08830"
     CDS             complement(5214..>5954)
                     /locus_tag="PLE23_08830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HMX83407.1"
                     /translation="QARVALSEANKIREIIDNQKGAIKGELRIGIIPTISPYLLPLFV
                     SDFITKYPEVELIIDELTSDKIIAKLSQDLLDAALMVTPINESTFIEMPLFYEKFLLY
                     LSENHPLLNKVYVNYEDIDLSDILLLKEGNCFRNQIVNLCGEYATHRRHQLKFDAGCL
                     ETIKKMVDRKQGFTILPELAVDGLTDLEKRRVRQFPSPEPVREVSLVVRRNFMKKNLL
                     ELFLNSIKENIPPHIVANPDINTIAWRD"
BASE COUNT         1625 a         1303 c         1150 g         1876 t
ORIGIN      
        1 gaggccgaga cgctggccaa gcgcttcggt tcggcgagca cacctgacct ctaccctttc
       61 catcccataa tctatgtcag taaccaactt gacgttactc ttcgatttaa catcgaaaaa
      121 actcggccag ccagtaccac tttcaaattt tgtgtctgat tcgaataatg gcaatctgca
      181 tgcagcacag taataggtcc cttttgcgtg attgtcccag tatttgccgg tgaaggcacg
      241 ctccgtgccg gattctcgca tcacattata acacatgggc gaaagttcct ttttccactc
      301 tgcctcggtt tttacaatag gttttatagt gtcattctcc acttccgaaa caagttttgt
      361 tgctggtttt gctgtttcag gtgttgaccg aagattgaag gtgcaagcat tcacaaaact
      421 tactaaaata caggctaggt aaatcattgc attcataata tgataaattt tattttcgct
      481 ttcgcatttt tggatgatgc cacacttcct tctgcgcgta ttcgatgaga ctttttactg
      541 ctttcatgac agaaatgaat accatatagc caaaagttat tactacgtaa aggaaagagt
      601 aagacccggc attaaaaaca ctcaagtccg acattgccca ggcaacaact cctgtcacaa
      661 cagcaagaat taaatataca acaaccgatg tgttccaata gtttggattg ttctctactc
      721 tcgtacttat tacgctatta aatatggcgt acaacatcaa aaatgtacag gcactcatcc
      781 agtaaaatct cggatatggt tctaccatac cggcacttac caatagaaaa aacccaaacg
      841 acaaaatcaa actgccgatg agactggtag tggccaactt tcgcggatta tcaagattat
      901 aggcaaccat ggttagttaa ttttttacta tcgtagcaaa gagatcaaat tcgttggcat
      961 catctatcct tacatggacg aagtccccga tccgtgcata attttcttta gcagataaaa
     1021 gcacctcgtt gtccacctcc ggcgaatcgc tttcagtacg gccaacaaaa taatctcctt
     1081 cttttttatc aatgagcact ttgaaggttt tccctatttt tccctgattc ttatcaaatg
     1141 aaatttcctt ctgaatctcc attatccggg cagcccttgc ttctttaatc ggggcaggca
     1201 catcatcttc catttggtgg gcaaatgtgt cttcttcatg actatattgg aatacaccaa
     1261 gtctgtcaaa ttccatttct tgtacgaatg tacacaaatc ctgaaattct tcttctgtct
     1321 cacctgggaa gccgacgatt agtgttgttc taatggaaat atccggtacg atccgtctgg
     1381 catgctctat caattcacgg gtttcctgct gggttatctg ccgtttcatc cgtttaagta
     1441 cctcgtcatt agcatgttgt actggtaggt caagataatt gcagacctta ggttgtgcag
     1501 ccattacatc cagcacttca agcggaaatt tgctcggata ggcataatgc aacctgatcc
     1561 attctattcc ttccacatca cacaatgctt caagcaaccg tgccaattca cgttttttat
     1621 acagatcgag cccgtagtat gtaagctcct gagctatgag catgatttcc tttacaccat
     1681 gtcttgcaag gtttttacac tctgcgacga gctcttcaat cggtcgcgaa atatgcccac
     1741 cacgcatcag cgggatagca cagaatgaac aagtgcggtt acaaccttcc gatattttta
     1801 aaaacgcata atgctgaggt gtggttatca ggcgttcgcc caataattca tgtttgtagt
     1861 cagcgttgag cttagccaga agtccaggca actcgagtgt gccgaaataa gcatctactt
     1921 ccggaatttc ttcctcaagg gcatctttgt atcgttgcga caagcaacct gtgacgtaca
     1981 gtttttctat ctcaccatcc gatttcttgg cagcatattc cagaatggtg ttgattgatt
     2041 cttgcttggc aaggtcaatg aatccacagg tattgatgat gactacattg gcatctgaag
     2101 tagtgtcttc gtgggttaca gcaaaatcat ttcctttgag ctgggtaatc aaattctcgg
     2161 agtcaaccag attttttgag caacctaggg taatgacgtt aactatatct tttctgagtg
     2221 tttttgtttt caagttgaag tcttaaacat ttacaaatat aacggtgttt gaatacaatc
     2281 taatcgtaaa aaggttctat atttgaaatc aaagcattta ttttatgaaa aatctccttg
     2341 cagccataat ctttctctgt ttttgttcca atatgcatgc acagattggc tttcaggttc
     2401 agtatcagag attccaggat acgagctata tactttccgg atcgggcttt aatgaatttg
     2461 ttcaagctga ccaattgggc tttggcatag attatgcttt taaacttaaa aattaccggg
     2521 tcgaattttt tcctgcaata ttgtatttca ataaaaaaat aaacaaaaat tccatcttcg
     2581 ataccgagtt aaatacgaat ataacaacac ttgggcttca gttcaataca cacatatact
     2641 tcctcgatat tgaaggcgac tgcaactgcc ctacctggaa caaagatggc agttttatga
     2701 aaaaaggtat attcgcctgg gtttcaataa atgcatacaa tacagttttt aagcaaaata
     2761 caccagctac tcgcaatatg tttacttacg gtgcatccgg tggtttaggg cttgacatcg
     2821 ggttgtctaa gcatctgaca ctcacgcctt ttgcgggact tgaacttgag caaaggcctg
     2881 ttgtagaatt tacagatttg aaggctgaga gagatacaaa atcaatgtac aaactcgacg
     2941 ccggaatccg acttggctgg cactggggca aataatcttt attagggaag atacaattgc
     3001 ctgaatgcct cccataacac cattccacca caaacagcta cattcagcga atgtttgaca
     3061 ccagcctgtg gaatttctat tgctgcatct gctatggcta tggcctcgtc cgacacacca
     3121 tctacttcat ttcccagaat aagagcgtat ttcctgtcat gatgaaacac gaatttttcc
     3181 agtgaaatac tttccgtagt ctgctcaata gcaatattgt aaaatccttc tttctcaagc
     3241 aatttgaggc aatcttcagt tgattcgaca taggtccagc ttacagtttc tgtcgatcct
     3301 agtgctgttt tcaatatttc gcggtgcggt ggttgtggtg taaatcccgt aataaaaata
     3361 tggcttaccg caaatgcatc acatgtgcgg aaaaccgaac cgacattgag acctgagcga
     3421 atattgtcaa gtaccaccac caatggcatc ttacggatgg tgtttacttt gtcggctgat
     3481 attcggtcaa gctcactggt agaaagtttt ctcatgccta tttcccctgc gattccttgt
     3541 aatcaatagc tgcctttaca aaagccacaa acagtggatg aggtttttcg actgtactct
     3601 ttaattcggg gtgatactgt acacccacaa accatgggtg atccggtatt tcaacaatct
     3661 caacaaggcc ggtttcagga ttgtgtccgg tagcctttaa tccattttct tcaagaatat
     3721 tgatgtattg attgttaaat tcaaatctgt ggcggtgtcg ttcattgatt tttgtctgac
     3781 catacgcttt aaaagctttc gaattcttta ataattcgca tttataggat cccaaacgca
     3841 tggttccacc tttttgagta agtgcttttt gcgattccat catatcgatt accggatatt
     3901 cggtatttgg gtcggcttca gtagtattgg cattggtaag cccggctgca tttcttgcaa
     3961 actctactac cgcacattgc ataccaagac agatgccaaa gaaaggaatt ttctcctcac
     4021 ggcatttttt aatggctacc agtttgccca acaaaccacg ttctccaaaa cctggcgata
     4081 cgagtatacc atcaagctta ttcaattttt cgatttcttt ttccgaatca ataagttttt
     4141 cagcgtggat tggtataacg tttaccttac aaagattgct tgcaccggca tgaatgaagg
     4201 cttcataaat tgatttgtag gcatcctgaa gctcgttgta tttacctatg agcccaatct
     4261 gtacctttga agtagggttc ttaagtttat caagaaatga attccatgca ctcaggtcaa
     4321 gttcttttgg tgaagtgagt ttcaattttt caagaacgat ggtatccagg ttttccttag
     4381 ccatcagcac gggcacagaa tagatcgttt cggcatccat tgcttctatc acagcttctg
     4441 tttcaacatt acagaagagt gctactttct tcctcaactc ttttggcaag gcatgttctg
     4501 tacgacatac cagtatatcg ggttgtacac ctgagcggag caattctttt accgaatgct
     4561 gtgttggttt ggttttcaat tccttagctg cccgtagata aggtatcaat gtaaggtgaa
     4621 tggttatgca gttatctggt ccaagctccc acctcaactg tcgtagtgct tctagataag
     4681 gcaatgactc gatgtcacca actgtaccac caagttcagt aataacgatg tcgtattttt
     4741 tgtcttgccc cagcagtttt accctgcgtt tgatttcatt ggttatatgt ggcactacct
     4801 gcactgtttt accaagataa tcgccacgtc tttccttatc tatcaccgaa cgataaatac
     4861 gccctgtagt cacgttattg gcctgacttg ttggtatgtt gagaaaacgc tcgtagtggc
     4921 cgaggtcaag gtcggtttca gcaccgtcat ctgtcacaaa acattcgcca tgctcgtaag
     4981 ggttaagtgt gcccggatcg acattcaggt aaggatcaaa cttttggata gttactttat
     5041 agcctctgct ttgaagcaat tttgcaaggg acgctgcaat aattcctttc cctagggaag
     5101 acgttacgcc gcccgtaaca aatacatatt tggtcattgg aagttggtgt tgttacgggg
     5161 tacaaaggta agagattatt tgaatgtttt atttgtagtg ctaaaataaa actttaatct
     5221 ctccatgcaa ttgtatttat atccggattc gcaacaatgt gtggtgggat attttcctta
     5281 atagaattta gaaacagctc aagcagattt tttttcataa aatttctcct tactaccagg
     5341 cttacttcgc gtacaggttc gggtgaagga aactgcctga cacgccgctt ttctaaatcc
     5401 gtgagcccgt caactgccag ctcaggcaat attgtaaaac cctgctttct gtccaccatt
     5461 tttttaattg tttctaaaca acctgcatca aattttagct ggtgccttct gtgcgtagca
     5521 tattcgccac aaagattgac aatctgattt ctgaaacaat ttccctcctt cagcaataat
     5581 atatctgaca aatcaatatc ctcgtagttg acataaactt tattgagcaa tggatggttt
     5641 tcggagaggt acagcaagaa tttctcataa aacaaaggca tttctataaa tgtactctca
     5701 ttgataggtg tcaccatcaa tgcggcatcc aataaatcct gactgagctt ggcgatgatt
     5761 ttgtcagacg tgagctcgtc aataatgagt tctacctccg gatactttgt aataaaatca
     5821 gaaacaaaca aaggtaacag atatggacta atggtaggaa tgatgcctat gcggagctca
     5881 ccttttatgg ctcctttctg gttgtcaata atttccctga ttttgttcgc ttcagacaag
     5941 gctactcggg cttg
//
LOCUS       DAOQQY010000178         5940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5789_length_5940_cov_4.839762, whole genome shotgun sequence.
ACCESSION   DAOQQY010000178 DAOQQY010000000
VERSION     DAOQQY010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5789_length_5940_cov_4.839762"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PLE23_08835"
     CDS             complement(<1..224)
                     /locus_tag="PLE23_08835"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HMX83408.1"
                     /translation="MLRKFPFLNLFILLWLLTPRTSSAQQVKAALIGFYNLENLFDTI
                     KAPGVIDDEFLPNGPRVWNTEKYTSKLKNMS"
     gene            577..3096
                     /gene="mgtA"
                     /locus_tag="PLE23_08840"
     CDS             577..3096
                     /gene="mgtA"
                     /locus_tag="PLE23_08840"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HMX83409.1"
                     /translation="MNTPDSPSFWQYDPEYWYKTLQSGKTGLTNDEALQKLKDIKGKQ
                     RVQSSFEKDVKLFVRQFSDPLMLMLIAAVILAGFLGETSDVVIIAFVVLASGILSFIQ
                     ERNAGKVVEKLQALLSLRSKVIREGELTEINSADVVPGDVVVISAGDIIPADCLITEA
                     NELYLNEASLTGESFPVRKSIGTLPIDTPLARRDNCTWEGTSVVSGSGKAVVINVGQN
                     TMFGNIVHNSNVHVETSFEKGIKDFGFLLMRITLVLSVFILVVNLLNHKSVIDSALFA
                     LALAVGMAPELLPAINTIAMSAGAKRMLDKKVIVKKLSSVQSLGEVNLLCTDKTGTIT
                     EGVINISKIVDTAGNPSDFVGKISYWNAAFESGYSNPIDDALKSLKPSADALPAKIGE
                     IPYDFNRKRLSIAVDTGQEKLLISKGAYNEISSICTSVRLSDGSAADFAKIRADIDQH
                     YLAYGTDGYRAIAVCYKPLDKTIISKEDESDMIFAGFILLNDPVKQDIPETLARLREL
                     KVGLKIITGDNVNVAKSIGRTIGIDDPVVMSGKEVISLTPEALRVKVRDTQIFAEVEP
                     MQKQSIILALKKSYTVAYMGDGINDVSAINAADIGISVSNAVAVAREAADFVLMEKDL
                     IVLAEGITEGRKTFANTLKYIYINTGSTFGNMCSVAVASLALPFLPMLPKQILITNFI
                     TDFPYLAVASDDVDTQQLQAPGKWNMKTIRNYMLIFGVHSSLFDMITFVTLLYFLKVQ
                     EAGFQTGWFVESTLTELCILFIIRTQNNFFKSKPGKWLSILSAVALVITIILPYTPLG
                     VQAGMLSMPLLNMGAIILIVVLYLLTTDLLKVWFFRKFKAG"
     gene            3234..5510
                     /locus_tag="PLE23_08845"
     CDS             3234..5510
                     /locus_tag="PLE23_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMX83410.1"
                     /translation="MKKILIPIFILFFISAIKAQTLYDINTIQDIKIEFTQSNWDYIL
                     DTAAAGKDEYVMAKYVIINGVVFFSVGVKYKGNSTYKPNQAKNPLHIELDTWKNQDYE
                     GYTDIKLSNVAFDPSFIREPLSYTILRQYMDAPQSNYANISINGALMGLYVSSESISK
                     KFVKKYFGNNNNAFFKCNPSGGGMPGGMSRPSLEYLGMDSTKYFKAYELKTDYGWKDL
                     VLLCNTLNNTPAQIESVLDVDRALWMLAFNNLFVNLDSYTGDFRQNYYLYKDDFGRFN
                     PIVWDLNMSFGIFSFAGGSTQLSTTKSKQEMNHLLHINESGWPLIQKLLANPSYKRMY
                     IAHLRTMLQENFSNGSYKTTAQEYHDLVTTSVQQDPKKFFSTQMFQNNLNADVNVGIF
                     IAPGIFDLMDGRANYLSNLADFKAVPPTITDISHSTFISQGSKVHITANVSNANPGIV
                     TFAFRYGTAGPFIKLKMEDDGQHNDGVAGDNIYGAEFDMDASLVQYYIYAENNSAGAF
                     SPERAEHEFYTIYANINVVEKGEVVINELMSNNETTVTDPTGNYSDWIELYNNTDQTL
                     SLKDAYLSDSYTEPLKWKFPDNTTIAPKGYLIIWASDTTQAGLNTNFKLSNNGEQLIL
                     SYENGFVTDSITFPSLKKDRSYARCPNGTGAFSKLDPTYSASNCASSAAGGLVLSDFS
                     IYPNPASGRVHFRLTVNNHSDLRIKLTDMSGLTLWQLNGNDINNGLDISQLSPGLYFI
                     ELAQLSTGKTSIRKLLKL"
     gene            5714..>5940
                     /locus_tag="PLE23_08850"
     CDS             5714..>5940
                     /locus_tag="PLE23_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83411.1"
                     /translation="MRKFFLLLSLFTCQYLFSQVAYQQIILDQNGRPISNVLVQDPDG
                     VCKTRTDTDGKFELNCYREVSSLQFILAGEL"
BASE COUNT         1809 a         1157 c         1196 g         1778 t
ORIGIN      
        1 gacatgtttt ttagtttgct tgtatatttt tcagtattcc atacgcgtgg accgtttggt
       61 aaaaattcgt catctatcac accgggtgct ttgatggtat caaacaggtt ttcgagattg
      121 taaaaaccaa tgagtgcagc ttttacttgt tgggcagatg atgttctcgg agttaataac
      181 cataagagta taaaaagatt taaaaacgga aattttcgca gcattgtaat ttttttacaa
      241 atatacattt aataactgag gatgtgactg aggctgtttt aagatatcgt taaaatctgt
      301 gtccaataaa tgtaagtaca aaaatcagga ttagatgtaa gtcaatgtat gagagcaaaa
      361 aacttaaacc ttgaaccatt cagatatgta tagtgtcagc tcaaaattgc gattttccag
      421 gttgaaatgc agtatttata tttttcccta tccattgcca ccgttaaagt aatattaaat
      481 tgctgttatt tatctttagt aaaatgtgtc tttggtaaat ttgtattgat aattttttgg
      541 ttcgatttta ttctgaatcc tcaaaatatt cctatcatga atactcccga tagtcctagt
      601 ttctggcaat acgatcctga atattggtac aaaaccctcc aatcgggcaa aaccggtctt
      661 accaatgatg aggctttgca aaaactcaaa gatattaaag gaaagcaaag ggtacaatct
      721 tcgtttgaga aagatgtaaa actttttgtc cgccagttta gtgacccgct tatgctcatg
      781 ctgattgcag ctgtaatcct ggcggggttt cttggtgaaa catccgatgt tgttataatt
      841 gcttttgttg tgcttgccag cggtatcctc agttttatac aggaaaggaa tgccggtaaa
      901 gtggtcgaaa aactccaggc attactttct ttgagaagca aggtcatacg cgagggggaa
      961 ttaacggaaa ttaattcagc agatgttgta ccgggtgatg ttgtagtcat aagtgccggc
     1021 gatattatac ctgccgattg tctgataacc gaagcgaatg aactctattt gaatgaagcc
     1081 agcctcaccg gcgaatcatt cccggtgcgc aagtcgattg gtacccttcc aatagacaca
     1141 ccgcttgcca gacgcgacaa ttgtacgtgg gaaggtacaa gtgttgttag tgggtcgggt
     1201 aaggctgttg tcatcaatgt cgggcagaat accatgtttg gtaatattgt gcacaactcg
     1261 aatgtacatg ttgaaacctc tttcgaaaaa ggcatcaaag atttcggctt tctgttgatg
     1321 cgcataacgc tggttctctc cgtatttatt cttgtggtca acttactcaa ccataaaagc
     1381 gtcatcgatt cggctttgtt cgcccttgcc ctagccgtag gtatggcacc tgaactgctc
     1441 cctgctatca ataccatagc catgagcgca ggggccaaac ggatgcttga taagaaagta
     1501 attgttaaaa aattatcttc ggttcagagc ctgggtgaag tcaatctcct ttgtaccgac
     1561 aagacaggta ccatcacaga aggtgtcatc aatatttcaa agatcgttga tacagctggc
     1621 aatcctagtg attttgttgg caaaatctct tactggaatg ccgccttcga atcaggatat
     1681 tcgaacccca ttgacgatgc cttgaaaagc ctgaagccgt cggcagatgc tttaccggcc
     1741 aaaattggcg aaattcctta cgatttcaac cgaaaacgtc tcagcatcgc tgtggatacc
     1801 ggtcaggaga aattgctcat cagcaaaggt gcttataatg agataagcag tatctgtaca
     1861 tcagtaaggc tgtcggatgg cagtgcagcc gattttgcta agataagagc tgatattgat
     1921 cagcattatt tggcctatgg caccgatgga tatcgggcaa tagctgtttg ttataaacca
     1981 ttggacaaaa caatcatatc aaaggaagat gagtcggata tgatttttgc cggtttcatt
     2041 ctgctcaatg accctgtaaa gcaggacatt ccggaaaccc ttgcacgcct tagagaactc
     2101 aaagtaggac taaaaattat tacaggcgat aacgtcaatg ttgcaaaatc cataggccgt
     2161 accataggca tagacgaccc cgtggttatg tcaggcaaag aagtcatttc actgacgcct
     2221 gaagcactcc gggttaaagt gcgtgatacg cagatatttg ctgaagtaga gccaatgcag
     2281 aaacaaagta ttatactggc attgaaaaaa tcctacacgg ttgcttatat gggcgatggt
     2341 atcaacgatg tgtcagccat caatgcagcg gacatcggca tatctgtaag taatgccgtg
     2401 gcagtagcaa gagaagcagc cgattttgtc ctcatggaaa aagaccttat cgtactggca
     2461 gaaggaatta ccgaaggtag aaaaacattt gccaatacac ttaaatatat atacatcaac
     2521 acaggatcaa ccttcggcaa tatgtgtagt gtagcggtgg catccttggc tctgcctttt
     2581 ttacctatgt tgccaaagca aatccttatt accaatttta ttacagactt tccttatctg
     2641 gcagtagctt ctgacgatgt tgacacccaa cagctacaag caccgggcaa atggaatatg
     2701 aaaaccatcc gtaactatat gcttattttt ggggtacaca gttctttatt tgatatgatt
     2761 acttttgtta ccttgttgta tttcctgaaa gtacaggagg ctggatttca gaccggctgg
     2821 tttgtagaat caacacttac ggagctttgt attcttttca tcatacgcac acaaaacaat
     2881 ttttttaaga gcaaacccgg caaatggctc agtatcctga gtgcggtagc ccttgtcatt
     2941 accatcattc tgccctatac gcctttaggt gttcaagcag gaatgcttag tatgcctttg
     3001 cttaatatgg gagcaataat attaatcgtt gtgttgtatt tacttactac cgacttgtta
     3061 aaagtatggt tttttagaaa atttaaggcc ggataattgt ttttataaac tctgaaaaaa
     3121 ctttgtatta attgtatagt atttttaagt gtgcgaatga ataaaaattt taattatcag
     3181 acttttttat tttgaaatat tgaatacctt tatgactcta atcaatattt ctgatgaaaa
     3241 aaattctgat tccgattttt attcttttct ttatttcggc aataaaagca cagacccttt
     3301 atgacatcaa taccattcag gacattaaga tagagtttac ccaaagtaac tgggattata
     3361 ttctggacac agctgctgca ggtaaagatg aatatgtaat ggccaaatat gttatcatca
     3421 atggtgttgt cttttttagt gtcggagtaa aatacaaggg caatagtact tataaaccca
     3481 accaggccaa gaatccacta cacattgagc ttgatacctg gaaaaatcag gattatgaag
     3541 gttatacaga cattaaactg agcaacgtgg cctttgaccc aagtttcata cgtgagccat
     3601 tgtcatacac cattttgcga caatatatgg atgcaccaca atcgaattat gcaaacataa
     3661 gcatcaatgg tgctttgatg ggactctatg taagttcaga atcaatttct aaaaagtttg
     3721 ttaaaaagta ttttggaaat aacaataatg ccttcttcaa atgcaaccca agcggtggtg
     3781 gcatgcccgg aggaatgtca agacctagcc ttgaatatct tggaatggac agcaccaaat
     3841 acttcaaagc atacgaactc aagacagatt atggctggaa agacctggtt ctgctttgta
     3901 atacactaaa caatactcct gcacaaatag agtcagtact ggatgttgac cgtgccctgt
     3961 ggatgctggc tttcaataat cttttcgtca acctcgatag ctatacaggc gatttcagac
     4021 agaattatta tctctacaag gatgattttg gccggtttaa tccaatcgta tgggatctga
     4081 acatgtcctt tggtattttt agttttgccg gtggttcgac acagttatcc actaccaaat
     4141 caaagcagga aatgaaccac ctgcttcaca ttaacgagtc gggatggcca cttatacaga
     4201 aactccttgc caacccaagc tacaagagaa tgtacatcgc acacctgaga accatgttgc
     4261 aggaaaattt ttcaaacggc agctacaaga caactgctca ggaataccac gacctagtaa
     4321 ctacctctgt tcagcaagat ccaaaaaagt ttttcagcac acaaatgttt cagaataacc
     4381 ttaatgcaga tgtcaatgtg ggaatcttca ttgcacccgg aatttttgac ttgatggacg
     4441 gacgagcaaa ttacctgtcc aatcttgctg attttaaggc agtaccacct accattacgg
     4501 atatttcgca cagcactttt attagtcaag gcagcaaagt gcatattact gcaaatgtta
     4561 gtaatgcaaa tccgggtata gttacctttg cattccgcta tggaacggcc ggtcctttca
     4621 tcaaactaaa gatggaagat gacggccagc acaatgatgg tgttgcaggt gataatattt
     4681 atggtgcaga gtttgatatg gatgccagcc ttgtacaata ttatatttat gccgaaaata
     4741 atagtgcagg agcattttca ccggaaaggg cagaacacga gttttatacc atttatgcaa
     4801 acattaatgt tgtagaaaaa ggagaagtgg tcatcaatga actcatgtct aataacgaaa
     4861 ccaccgtcac tgacccaacc ggcaactata gtgactggat agaactatac aataatacgg
     4921 accaaaccct cagtcttaaa gatgcctatc tgtctgattc ttataccgag ccactcaaat
     4981 ggaagtttcc tgataatact acgatagcac ctaagggtta cctgataatc tgggcaagtg
     5041 acacaacaca ggcaggattg aatacaaact tcaaattatc gaacaatgga gaacagttga
     5101 tcttgtctta tgaaaatggt tttgttacag acagcattac ttttccatct cttaagaaag
     5161 atcgctctta tgcccggtgc ccgaacggta caggtgcttt ttcgaagctc gatcctacgt
     5221 acagtgcttc caattgtgca tcatccgctg ccggtggttt ggtattgtct gacttcagta
     5281 tctacccaaa tcctgcttct ggcagagtac attttcgctt aactgtaaac aatcattctg
     5341 acctgcgtat taagctgaca gatatgagtg gacttacact ttggcagtta aacggcaatg
     5401 atatcaacaa cgggcttgac atctctcaac taagcccggg attatatttt attgagctgg
     5461 cgcaattgtc aaccggaaaa accagcatcc gtaaactatt gaagttgtag aaaagatttc
     5521 ttgtatcgct gaatgacacg agcaactact cgaacctatc tgatattttt ttcatttgat
     5581 tctgttgaaa tgtgaatatg aatatttact gaaaattttt cataaaaatt tcttcaacga
     5641 ataaaataaa attgctcaaa tcagaaaaga atgtgcataa ctaatagtta tatttgtctt
     5701 tcaattgatg actatgagga aatttttctt gctattatct ttgtttactt gccagtatct
     5761 tttttcacag gtagcatatc agcaaataat tcttgatcag aacggccgac ctatcagcaa
     5821 tgtactcgtt caagacccgg acggtgtttg caaaaccagg acagacacag atggtaaatt
     5881 tgaattgaat tgttatcggg aagtatcttc cttacaattt attcttgcag gtgaactgtt
//
LOCUS       DAOQQY010000179         5861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_5927_length_5861_cov_5.265932, whole genome shotgun sequence.
ACCESSION   DAOQQY010000179 DAOQQY010000000
VERSION     DAOQQY010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5927_length_5861_cov_5.265932"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="PLE23_08855"
     CDS             complement(<1..639)
                     /locus_tag="PLE23_08855"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMX83412.1"
                     /translation="MTRIYCLILLLIPFVVGSQNIDLILHNGKIFTAEPDKTSATAIA
                     VKGKRIYRIGDESILDLRGPNTIVIDLKGKFCMPGLIEGHGHMNGLGASLNSIDLLDA
                     QSWDEAIHAVKLKVAESNKGRWIEGRGWHQEKFKKLPLDAVGGYPSQASISAVSPDNP
                     VILVHASGHALFANAKAMSLAGIDRNTPDPAGGKIIRDKNGNPTGIFEENAME"
     gene            complement(683..2608)
                     /locus_tag="PLE23_08860"
     CDS             complement(683..2608)
                     /locus_tag="PLE23_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMX83413.1"
                     /translation="MQHKINILLIEDNKTDQWTISKLLEGTKLRYNLLKADTLLDGLE
                     FLINSSVDVILLDLGLPDSNGYKTLQRIIEKNYLIPIIVLTVTQNSIIESLAIKAGAQ
                     EYLTKDKLNQSILLKAIQNSYLRNSIVKKLISQNDELNSKLKNQTEAWALTGSAFWSM
                     DLVTNEMQWSKELFEMFNFKVDSVNPSLSLYLKYVHPEEKEQVNKWFGNVPASYSSQE
                     LIHRVIIGKSIIKIRLIARFCIDRKAHPLRIIGTIQQIKTDAASEAKPIDEGLTESNI
                     SQFIESLIQNIEFSMKQVQLIHLDAENTLIPDTPPGIIEKFHVSMKVLNDTMNWLKGI
                     MLLSGFRESNSGTLISIKSLIRKTEKHLGYKMLEHSSLDEHLTNLQVEYSPPIWEVLL
                     KALNNWLTQIESHNQKILEMTVFQKNNEAKLSFTANLTNVRADFIKKTASGIENQRDL
                     KLTSMFNEASMTVNEYAVLIYNITKWMGGTMNLIRNEAERIVEINIPLRINILQSNKT
                     TNHRNISDNYKILIADDHFLTRLSTKKNIKSILGDIMIDEAENGEQAIMKAEKEKYDL
                     ILMDIEMPGMDGKESMLEIKKIYNPTIIALVHEDAEAKQELIFEGFAGVLKKPIRTEE
                     LTEVINRQSQNLLSYYE"
     gene            complement(2636..3034)
                     /locus_tag="PLE23_08865"
     CDS             complement(2636..3034)
                     /locus_tag="PLE23_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83414.1"
                     /translation="MINKYKPNLRKEDKMPGNSPLPVGTAKSLTIGWSPHSAFAKGQK
                     NISFTQTFSRKNYSRHSIARLLWVCVLKKYSYTKSRVSELSKIINATTHILYMYEMAT
                     IGMNAHFVIRYNESGFVRVNNKVYWVTIKI"
     gene            3113..4090
                     /locus_tag="PLE23_08870"
     CDS             3113..4090
                     /locus_tag="PLE23_08870"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMX83415.1"
                     /translation="MQTFSQFLKIIENFVSEQRFWGKNTSLYEPIEYMFSLPAKRVRP
                     ALMLLGYQLFSKDYQKALPIAFAYEAFHNFTLIHDDIMDQAPLRRGFPTIHIKYNTNA
                     GILSGDALLINVYEYIRKHTPSEKLDKVLECFNKMAIEVCEGQQMDMEFEKAQVVSLT
                     DYLEMIQFKTAVLLGASLSTGAILADASDADTEFCYELGTNLGMAFQMQDDYLDVWGD
                     QNQTGKQKAGDIINGKKSAPYILALQVLPEYEKQTLINIYNTDKPQQEEKIKIVLGLF
                     EQAGAQQAIRDLKNVYHSKTVQLLEHISAPDENKKHLHDLIELLLDRNV"
     gene            complement(4073..4573)
                     /locus_tag="PLE23_08875"
     CDS             complement(4073..4573)
                     /locus_tag="PLE23_08875"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX83416.1"
                     /translation="MADKQKQSNFTIAKATEKDIPGIFDCVKELALFEMAPEAVITNP
                     VVYLEDWLKGWFEVLVAKSDDRIVGIALYHPAYSSWKGRMMYLDDLIVTESYRGLGIG
                     KALLDAFITEAKKSNAVICKWQVLDWNKEAVVFYEKIGAELDKGWWNVKLMTLSYSLW
                     QSDIAV"
     gene            4770..5144
                     /gene="rpsL"
                     /locus_tag="PLE23_08880"
     CDS             4770..5144
                     /gene="rpsL"
                     /locus_tag="PLE23_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HMX83417.1"
                     /translation="MPTINQLVRKGREEIVETSKSRALNSCPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNSIEVIAYIPGEGHNLQEHSIVLIAGGRVKDLPGVRYTIVRGAL
                     DTAGVNNRKKSRSLYGTKRPKK"
     gene            5180..5647
                     /gene="rpsG"
                     /locus_tag="PLE23_08885"
     CDS             5180..5647
                     /gene="rpsG"
                     /locus_tag="PLE23_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HMX83418.1"
                     /translation="MRKRKPKVRIIAADPRYSDPMITQFVNNLMYEGKKSTAFKVFYD
                     AMDVVKEKAGESELEVWKKALNNVMPQVEVRSKRIGGATFQIPTEIRAKRKVSMGMKW
                     LIKYSRARSGKGIAEKLANEILAASRNEGAAVKRKEEMHKMAESNRAFASFRM"
     gene            5686..>5861
                     /locus_tag="PLE23_08890"
     CDS             5686..>5861
                     /locus_tag="PLE23_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMX83419.1"
                     /translation="MARDLTFTRNIGIAAHIDAGKTTTTERILFYTGVSHRIGEVHDG
                     AATMDWMEQEQERG"
BASE COUNT         1781 a         1127 c         1089 g         1864 t
ORIGIN      
        1 ttccatggca ttttcttcaa atatcccggt cggattacca tttttatctc gtattatttt
       61 cccacctgca ggatctgggg tgtttctgtc tattccggca aggctcattg cttttgcatt
      121 cgcaaacaat gcatgcccgc tggcatgtac tagtattaca ggattatctg gcgatacggc
      181 acttattgaa gcttgtgacg gatatcctcc tactgcgtca aggggcaatt ttttaaattt
      241 ttcctgatgc caacctctgc cttcgatcca tctgcctttg tttgattcag ctactttgag
      301 cttaacagca tgaatggcct cgtcccatga ctgagcatca agtaggtcaa tgctgttgag
      361 actagctccc aaaccgttca tgtggccgtg cccttcaatc aatccgggca tacaaaattt
      421 ccctttcagg tctatgacaa tcgtgttagg acctcttagg tcaagtatgc tttcatcgcc
      481 aatacggtaa atccttttgc cttttacagc gatagccgtt gcacttgttt tatctggttc
      541 tgcagtaaaa atcttcccgt tgtgcagaat aaggtcaata ttttgtgaac cgacaacaaa
      601 cggaattaac aataaaatca agcaataaat cctggtcatt accttaaagt tttctcaaag
      661 ataatatcca ttgctatttt atttattcgt aataactcaa taagttttgt gattgccgat
      721 ttatcacttc cgtaagttct tctgttctga ttggtttttt taatactcct gcaaagcctt
      781 caaagattaa ttcttgcttg gcttcagcat cttcatgtac aagagctatt atggttgggt
      841 tgtaaatttt tttgatctcc agcattgatt cttttccatc cataccgggc atttcaatat
      901 ccatcagtat cagatcgtac ttttctttct ctgctttcat gattgcctgt tcaccgtttt
      961 ctgcttcatc tatcattatg tctcccaata tactttttat attttttttc gtcgaaagcc
     1021 ttgtcaggaa atgatcgtct gcaatgagaa ttttataatt atcagatata ttccgatgat
     1081 tagtggtttt attactctgg agaatattga ttctcaatgg aatatttatt tcaacaatcc
     1141 gctcagcctc attccgaata agattcattg ttccacccat ccattttgta atattatata
     1201 tcaatacggc gtactcattt accgtcatgc ttgcttcatt aaacatcgat gtgagtttta
     1261 aatcacgttg attttctatt ccggaggcag ttttcttaat aaaatcggca cggacattgg
     1321 tcaaattcgc tgtaaagctt agcttggctt cattattttt ttgaaatact gtcatttcca
     1381 atattttttg gttgtgactt tctatttgtg taagccagtt gttcaatgcc tttaacaaaa
     1441 cttcccatat tggaggtgaa tattccactt gcaagttggt taaatgttca tcgagtgatg
     1501 aatgttcaag cattttgtag cctaaatgtt tttctgtttt tctgattaat gattttattg
     1561 aaataagtgt acccgaatta gattctctga accccgacag cagcattatc cctttcagcc
     1621 agttcattgt atcgttcagt actttcattg atacatgaaa tttctctatt atcccaggtg
     1681 gtgtatctgg tattaaggtg ttttctgcgt ctaaatgaat gagctgtacc tgcttcatag
     1741 aaaattcaat attttgaatc aaagactcaa taaattgact aatattgctc tctgtaaggc
     1801 cttcatcaat tggctttgct tcactggctg catcggtttt aatttgctga atagtaccta
     1861 tgatacgcaa tggatgtgct tttcggtcaa tacaaaacct ggcgattagc ctaattttaa
     1921 taatcgattt tccgatgatt actctgtgta ttaattcttg tgaagaatag gatgcaggaa
     1981 cattgccaaa ccacttgttc acttgttcct tctcttcagg atgcacatac tttaagtaca
     2041 aggaaagact agggttcaca ctatcgacct tgaagttgaa catttcaaac aactcctttg
     2101 accactgcat ctcattggtc acaaggtcca tcgaccaaaa ggcactacct gttaatgccc
     2161 atgcttctgt ctggtttttt aacttggagt tcaattcatc attctgagaa ataagctttt
     2221 ttacgatcga attgcgcaaa tacgaattct ggattgcttt tagtagaatt gactggttga
     2281 gcttgtcttt tgtcagatat tcttgggcac ctgcttttat ggcgagtgat tcaattatag
     2341 aattctgtgt caccgtcagc acaatgattg gaattaaata atttttttcg ataattcgct
     2401 gcaaagtttt atagccattg ctgtcgggta gtccgagatc aagaagaata acatccactg
     2461 atgagttgat gagaaattca agtccatcga gtaaagtgtc tgccttaagt agattgtatc
     2521 tgagcttagt tccttcaagt aattttgata tcgtccactg gtccgttttg ttgtcttcta
     2581 ttagcagtat gtttattttg tgttgcatgg ttatttccta gttattcatg atttgtcaaa
     2641 tttttatggt aacccagtac accttattat tcactctaac aaatcctgat tcattgtatc
     2701 ggattacaaa gtgtgcgttc ataccgattg ttgccatttc atacatatat aatatatgtg
     2761 tggtcgcatt gataattttg gaaagctcag aaaccctact tttcgtgtac gaatatttct
     2821 tcaaaacaca gacccaaagc agtcgggcaa ttgagtgtct cgaataattt ttccttgaaa
     2881 aagtctgggt gaaagaaatg tttttttgac ctttggcaaa tgcggaatga ggagaccaac
     2941 caatagtcag gcttttggcc gtgcctacag gtaatggact gttgccgggc atcttgtctt
     3001 ctttacgaag atttggcttg tatttgttga tcacctttgt ttttttgtgt atgtttacct
     3061 aaatgaacga caaggcagaa attctgctta gctttggcgc ctaaatatta ctatgcaaac
     3121 atttagccaa tttttaaaaa tcattgaaaa ttttgtctct gaacagagat tttggggaaa
     3181 gaatacatct ttatacgaac caattgaata tatgttcagc ctaccggcca aaagagtacg
     3241 ccctgctctt atgctgctag ggtatcaact tttcagcaaa gactaccaaa aagcactgcc
     3301 aatcgcattc gcttatgagg catttcataa tttcacactc atccatgatg atataatgga
     3361 tcaggcacct ttacgaaggg gctttcctac catacatatt aaatataata ccaatgccgg
     3421 cattcttagt ggagacgcac ttttaatcaa tgtgtatgaa tacatcagga aacatacacc
     3481 atccgaaaaa cttgacaaag tactcgaatg ctttaataaa atggcaatag aagtttgtga
     3541 gggacagcaa atggatatgg aatttgaaaa agcacaagtt gtctctctaa ctgattatct
     3601 tgaaatgatt caattcaaaa ccgcagtact actcggagct tcgctttcca ccggggccat
     3661 cctggcagac gcatctgatg cggataccga attttgttac gaactcggta ccaatcttgg
     3721 gatggcattc caaatgcagg acgattatct cgacgtatgg ggcgatcaga accaaaccgg
     3781 caaacaaaag gcaggtgata taatcaatgg taaaaaatca gcaccctata tattggcact
     3841 acaagtcttg ccggaatatg aaaaacagac actcattaat atatataaca cagacaaacc
     3901 tcagcaagaa gaaaaaatca aaatcgtgct gggtcttttt gaacaagccg gcgcccaaca
     3961 agctataaga gatttgaaaa atgtttacca tagtaaaaca gtgcaactac tcgagcacat
     4021 atcagcacct gatgaaaata aaaagcactt gcacgacttg atagaattat tattagaccg
     4081 caatgtctga ttgccataaa ctataagata atgtcatcag ctttacattc caccagcctt
     4141 tgtcaagttc ggcacctatt ttttcataga ataccacagc ttctttattc caatcaagta
     4201 cctgccattt gcatataaca gcattcgatt tcttagcctc agtgatgaat gcatcaagca
     4261 gagctttacc tatgccaagc ccacggtacg attctgtcac aatcaggtcg tcaagataca
     4321 tcatgcgacc tttccaggaa gaataagcag gatggtataa tgcaatacct actattctgt
     4381 catctgattt tgctactaaa acttcaaacc agccttttaa ccagtcttca agataaacta
     4441 cagggttggt aatgaccgcc tcaggagcca tttcaaacaa agccagctct tttacacagt
     4501 cgaaaatacc aggaatatcc ttctcggttg ctttagctat agtgaaattt gattgttttt
     4561 gcttgtctgc catattgaat ttgcttttta gaaaaaatat ttataaaaaa atcagatttt
     4621 ttttaaaaac atgtttgatt tgaacaaata tagttatatc tttgcaatcg cttttgaaat
     4681 aggtatgaaa atatctgaat aaaaaagtta ttagttaaaa atatttataa cataaacagt
     4741 tgattttaag acgttcataa tctttttata tgcctactat caatcaatta gtcagaaaag
     4801 gaagggaaga aattgtagag acaagtaagt cacgtgcatt aaactcatgc ccacaaaagc
     4861 gtggcgtgtg tacacgtgta tatacaacaa caccaaaaaa acctaactct gcgctacgaa
     4921 aagttgcaaa ggtacgtctt accaacagta ttgaggtcat cgcctatatt ccgggagaag
     4981 gccacaatct gcaggaacac tcaatcgttt tgatagctgg tggcagggta aaggaccttc
     5041 cgggtgtaag atataccatc gtaaggggtg cactggatac agcaggtgtt aacaacagga
     5101 aaaaaagccg ttctttatac ggtacaaaga gacctaagaa ataattgacc ataaattaaa
     5161 cagaaataga gtttaagtaa tgagaaaaag gaagccaaaa gttagaatca tcgcagcgga
     5221 cccacgttac agtgacccga tgataactca gtttgtcaat aacttgatgt acgaaggtaa
     5281 gaaatcaact gcttttaaag tattttatga tgcaatggat gttgtaaaag aaaaggcagg
     5341 tgagagcgag ctggaagtat ggaaaaaggc tttgaataac gtaatgccac aagtagaggt
     5401 aagaagtaaa agaataggtg gagcaacttt tcagattcca acagagatca gagcaaagag
     5461 aaaagtttca atgggcatga aatggcttat caaatattca cgcgccagaa gtggtaaagg
     5521 tattgcagaa aagttggcaa acgaaatact tgcagcaagt agaaatgaag gtgctgcagt
     5581 taagagaaaa gaagaaatgc acaaaatggc tgaatccaat cgtgcattcg caagttttag
     5641 aatgtaatta ttaattataa tcctttttca aaccgactat aagtcatggc tagagattta
     5701 acttttacaa gaaatatcgg aattgcagca cacatcgatg cgggcaaaac cactaccacc
     5761 gaacgaattt tgttctatac cggtgtatct caccgcattg gagaggttca cgatggcgca
     5821 gcaacaatgg actggatgga acaagagcag gaaagaggta t
//
LOCUS       DAOQQY010000180         5809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6028_length_5809_cov_5.794925, whole genome shotgun sequence.
ACCESSION   DAOQQY010000180 DAOQQY010000000
VERSION     DAOQQY010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6028_length_5809_cov_5.794925"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..968)
                     /locus_tag="PLE23_08895"
     CDS             complement(<1..968)
                     /locus_tag="PLE23_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMX83420.1"
                     /translation="MTLIKSISGIRGTIGGKKGDNLTPIDIVECAAAFGTWTIRNSGI
                     RKIVVGRDGRISGQMVSSLVINTLQALGIEVIDLGLSTTPTVEIAVPLEQAGGGIILT
                     ASHNPAHWNALKFLNSKGEFISAKDGEMVLQLIDEGNIEFVGVNNLGVCHKDDSYIHK
                     HVEQILALESVFSEKIKQKKYKIVLDAINSTGAIAVPALLDQLGCTTIVLNGEITGNF
                     AHNPEPLPEHLSQIREKVLAEKADLGIVVDPDVDRLAFVCEDGTLFGEEYTLVAAADH
                     ILSLRPGNTVSNMSSTRALRDITEKSGGIYQPAAVGEVNVVTKMKE"
     gene            1075..1809
                     /locus_tag="PLE23_08900"
     CDS             1075..1809
                     /locus_tag="PLE23_08900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMX83421.1"
                     /translation="MQQKEWFSSWFDTKYYTLLYRNRNDDEAKEFVRQLMKYYKPKKN
                     TEMLDMGCGRGRFARFLHEYEMKVTGMDISPQLIKKATVASPSEIEFVAHDIRKPFGK
                     LEYDFIFSFFTSFGYFDTDEEHLLTLQNVYEALKIHGIFLIDYLNADRISLNLPQKSE
                     VTIENINFSLHKYFKNGYFVKEIQVDDHGRIYNYVEKVRAFSLRDFKNLFSRLGLQIT
                     ACFGDYDLSSFDILQSRRLIIACVKR"
     gene            1836..2405
                     /locus_tag="PLE23_08905"
     CDS             1836..2405
                     /locus_tag="PLE23_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMX83422.1"
                     /translation="MYDVDFQIFHFVNDGLSNTFFDWVAPVLREKKTWIPLYIALLIW
                     LIYKYQKKSVLIIGLIIAVAGITDYTSSSIIKPFVKRVRPCNDQLIELQVNERVTCGA
                     GYSFPSSHAANHFAIAFFLSLTIFRKYRLMQWLSFLWAALIALSQVYVGVHYPADIFG
                     GAVLGMLIAMMVYYVSSCLWEEYLNYIET"
     gene            2402..3145
                     /locus_tag="PLE23_08910"
     CDS             2402..3145
                     /locus_tag="PLE23_08910"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HMX83423.1"
                     /translation="MSVTEHIILIGSILLTGVLSMAFYGKVKKLSKVLLAFSGAFLVS
                     ITFLHLFPIIYHSDNHSIGIWVIAGFFGQLFLEQFSSGVEHGHSHIHSHQNSNVLIMQ
                     VMFGLTIHSFVEGLSLGGFEMHGHEHDHHSHLLSEDVNPLLVGIVLHHVPAAFALMMV
                     FASSLVSKWKILLCLTIFAIMPSLGALTVIHFNFDQEVILMILAFVSGSFLHVATTIL
                     FETDNTNHKIPYKTLLAALSGVLLSLLTV"
     gene            complement(3149..4579)
                     /locus_tag="PLE23_08915"
     CDS             complement(3149..4579)
                     /locus_tag="PLE23_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMX83424.1"
                     /translation="MFRKIFTIAAFSILTSMLTSYVMIMSFSNSRNNFPREFSQVSLT
                     DFSQNHAPSIRRSNFSASAPTNFIKASSIATPAVVNIQASAKTEFDIWKFGAGKSSGS
                     GVIIAPEGYIITNRHVIDQAKTLRVTLADNREYKAELIGQDPSTDIALLKIDAKNLPA
                     ISLGNSDSVQVGEWVLAVGNPFNLSSTVTAGIVSAKGRSIDVLDDVYRIESFIQTDAV
                     VNPGNSGGALVNTRGDLIGINTAIMTKTGQYEGYSFAIPVNVVKKVIKDLLEYGKVQR
                     GILGIGPEDVTSDLAKSLRLPEVSGVYIGRVNPDGAAERGGLRSGDVITKINKKQIES
                     VPQLQEVLATFGPGDKLVIEYYRNGSRFVTPVVLQNKINTTAQLSAPEVDLQNELGFE
                     LRELNRVEKAHYNISGAYVHSITKKTVIGKTNMEAGFIINQVNNIKVNSVLQALNEIS
                     KIKGKVRLDGFYIDYPGKYSYEFYRE"
     gene            4697..5494
                     /gene="dapF"
                     /locus_tag="PLE23_08920"
     CDS             4697..5494
                     /gene="dapF"
                     /locus_tag="PLE23_08920"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMX83425.1"
                     /translation="MLKFYKYQGAGNDFIIFDNREGIFPLINRQKIITRLCDRRFGIG
                     ADGVMLLENAAGMDFNMIYFNADGNESSMCGNGGRCLVSFANDLGIIQQNAAFNAIDG
                     AHDAVYFSPQKVNLRMKDVSEIQKGNGFAVLDTGSPHYVRFVDDLSVVNVYEEGRQIR
                     YSPEFAENGINVNFVHKTEDILHVYTYERGVEDETLACGTGVTAAAIAWNEVFGHKSE
                     DSVPIMAKGGDLTVSFRKVGNTYTDIWLSGPAVKVFEGVIDLNHIPH"
BASE COUNT         1612 a         1106 c         1245 g         1846 t
ORIGIN      
        1 atttccttca tttttgtcac cacatttact tcaccgacgg cggcaggctg gtagatacca
       61 cccgattttt cggtaatatc gcgaagggct cgcgtagaag acatattcga gaccgtattg
      121 ccgggacgaa gagataagat atggtcagca gcagccacga gtgtgtattc ttcgccaaaa
      181 agagtaccgt cttcgcatac gaaagcaagt ctgtccacat ccgggtcaac tactataccc
      241 agatcggctt tttcggcgag gactttttca cggatctggc tgagatgctc tggaagcggc
      301 tcaggattat gggcaaaatt accggttatt tcgccgttga ggacgatggt cgtacaacca
      361 agttggtcaa gcaaggccgg tacagcaata gcacctgttg agttgatggc atccagcact
      421 attttgtatt ttttctgttt gattttttct gagaatacag attcgagtgc aagtatctgt
      481 tcaacatgct tatgaatata ggaatcatct ttgtgacaaa caccaagatt gtttacaccg
      541 acaaactcga tattaccttc gtcgatgagc tgtaagacca tttcgccgtc tttggcagag
      601 ataaattcgc ctttgctgtt cagaaattta agggcattcc agtgtgcggg attgtggctg
      661 gcagtcaata tgatgccacc accggcttgt tctagcggaa cggctatttc tacagtaggt
      721 gtagtggaca ggccaaggtc aatcacttcg atgccaagtg cttgcaaagt atttattaca
      781 agagaagaaa ccatttggcc ggatattctg ccatctcttc ctaccacaat tttccggata
      841 cctgagtttc ggatggtcca ggtgccgaaa gctgcagcac attcaacaat atcaatggga
      901 gtgaggttat ctcctttttt accgcctatg gttccgcgga tacctgaaat agattttatt
      961 aaagtcacaa tataattttt tataactttg caaaaatact taaatgtttg aatgcggagg
     1021 ttgatttatt gaaggaataa atctcatgca aaaaatgtag tttaataaga ggtaatgcaa
     1081 caaaaagagt ggttttcatc gtggtttgat acgaagtatt acacactgct gtatcgcaat
     1141 cgcaatgacg acgaagccaa agagtttgtc cgtcagttga tgaaatatta taagcctaag
     1201 aaaaatacgg agatgttgga tatgggctgt ggcagaggtc gttttgcgag attccttcac
     1261 gagtatgaaa tgaaggtaac aggaatggac atttcacctc aactcatcaa aaaggctacg
     1321 gtagcgtcac catccgaaat agagttcgtg gctcatgata tcagaaagcc atttggaaaa
     1381 ttggaatacg attttatttt ttcatttttt acaagtttcg gatattttga cacagatgaa
     1441 gagcatttgt taacccttca gaatgtatat gaggcattga agattcatgg tatttttctg
     1501 atagattatc tgaatgcaga caggatttca ctcaatttgc cacagaaaag cgaagtgacg
     1561 atcgaaaata taaatttcag cctacacaaa tacttcaaaa atggttattt tgtaaaagaa
     1621 atacaggtgg atgatcatgg caggatatac aattatgttg aaaaggtgag ggcattttct
     1681 ttacgcgact ttaaaaatct ttttagtagg ttaggtcttc agataacggc ttgctttggt
     1741 gattatgatc tgagcagttt tgatatacta cagtcaagga gactgattat agcatgtgtc
     1801 aaacggtgag gcgtaaataa atcggtagca tacttatgta cgatgttgac tttcaaattt
     1861 ttcactttgt caatgatggc ctgtcaaata ctttttttga ctgggtggca ccggtattaa
     1921 gagaaaagaa gacatggata ccattgtaca tagctttact gatatggttg atctacaaat
     1981 accagaaaaa atctgttttg attatcgggt tgattattgc ggtagccggc ataacggatt
     2041 atacttcaag tagtattatc aaaccatttg tgaaacgagt cagaccctgt aatgaccaat
     2101 tgatcgagtt gcaggtaaat gagcgcgtaa cttgtggtgc aggctacagt tttccgtctt
     2161 cgcatgctgc caatcatttt gctattgctt tttttctttc gctaaccatt tttcgaaagt
     2221 ataggctgat gcagtggctg tcatttttgt gggctgcgct gattgcattg agtcaggtgt
     2281 atgttggtgt acattatccg gcagatatat ttggaggagc agtattgggt atgcttatcg
     2341 ctatgatggt ttattacgta tcttcgtgcc tttgggaaga ataccttaat tatatagaga
     2401 catgagtgta acagaacata taatattgat tggttcgatt ttacttaccg gcgtgctgtc
     2461 tatggcgttt tatgggaaag taaagaagtt gtcaaaggtt ttacttgcat tcagcggagc
     2521 ttttttggtt tccattacct ttctacattt gtttccgatc atttaccaca gtgataacca
     2581 cagtattggc atttgggtaa ttgccggatt ttttggtcag ttgtttcttg agcagttttc
     2641 atcgggtgta gaacacggtc actcccatat acatagccat caaaactcca atgtgcttat
     2701 catgcaggtg atgtttgggc ttacgataca cagtttcgtt gaaggattgt ctttaggagg
     2761 atttgaaatg catggacacg aacatgatca tcattctcat ttgctgagtg aagatgtaaa
     2821 tccattgctg gtaggtatag tgctgcatca tgtaccggct gcttttgctc tgatgatggt
     2881 ttttgcttcc tcgctggttt ctaaatggaa gattttgctt tgccttacca tatttgccat
     2941 catgccatca cttggtgcac ttacggtcat acattttaat ttcgaccaag aagtcatcct
     3001 catgattctt gcattcgtgt caggttcatt tttgcatgta gccactacta ttcttttcga
     3061 aacagacaat acgaatcaca aaattcccta caaaacgcta ttggcagcct tatcgggtgt
     3121 cttactttca ttgcttacgg tatagcagtt attctcggta aaactcgtat gaatatttgc
     3181 ccggatagtc gatatagaat ccgtccagtc ttacctttcc tttgattttg cttatttcat
     3241 tcaatgcttg taataccgag ttaactttaa tattgtttac ctgatttatg ataaaacccg
     3301 cctccatgtt ggttttgcct atgacggttt tttttgtgat agaatgtaca taggcacccg
     3361 aaatattata gtgtgctttt tcaactcggt tgagttccct gagttcgaat ccaagttcgt
     3421 tttgcagatc aacttccggt gcggacaact gtgcggtggt atttattttg ttttgtaaaa
     3481 ccacaggagt tacaaatctt gacccattgc ggtaatattc gatgacgagc ttgtcacccg
     3541 gaccaaaagt agcgagtact tcctgtagtt gcggtacaga ttctatttgt tttttgttga
     3601 ttttggtaat tacatcacct gacctcagcc caccacgttc agcagcaccg tcagggttaa
     3661 ccctgccaat atatacaccc gacacttcag gcagtctgag cgattttgcc aggtcagatg
     3721 ttacatcttc aggcccaata cccagaatgc ctctttgaac cttgccatat tctaataggt
     3781 ctttgatcac tttttttacc acattgaccg gtatggcaaa agagtagcct tcgtactgtc
     3841 cggttttggt catgattgca gtgttgatgc ctataagatc gcctcgcgta tttaccaaag
     3901 caccaccgct gttgcccgga ttaaccacag catctgtctg aatgaaagat tcgattctat
     3961 acacatcatc caatacgtca atgcttcgac cctttgcact tacaatgccg gcagtaacag
     4021 tagagctcag gttgaaaggg tttccaacgg ctagtaccca ttcacctacc tgtacagaat
     4081 ccgaattgcc aaggcttatg gcgggtaaat ttttagcatc gatctttaat aaagctatat
     4141 ctgttgacgg atcctggcct atcagttctg ccttgtactc gcggttatca gcaagcgtca
     4201 cacggagtgt tttggcttga tcaataacat gcctattggt gatgatgtac ccttcaggag
     4261 caatgatgac ccctgagcct gaagattttc cggcaccgaa tttccatata tcaaattcgg
     4321 tttttgcgga agcctgtata ttgacgactg caggtgtagc gatagatgaa gctttaatga
     4381 aattggtcgg tgctgatgct gaaaaattgc ttcgcctaat actcggggca tgattttggc
     4441 tgaaatctgt gagactcact tgtgaaaatt cacgcggaaa gttgtttcgg ctattactaa
     4501 aactcataat catgacataa cttgtaagca tactcgtaag aatgctaaaa gctgctatgg
     4561 tgaaaatttt tctgaacatg atattattaa tttgaagtaa aacgttttat atttatcgta
     4621 aattattctg tacatctatt ttacaataaa aaaggcatct ttgtttaact tacggatgct
     4681 tctaatggct aaagtaatgt taaaatttta caaatatcaa ggtgcgggca atgattttat
     4741 catttttgat aaccgtgaag gtatttttcc actcatcaac agacaaaaaa taattacccg
     4801 actgtgcgac aggcggtttg gtataggagc agatggtgtt atgttgctcg aaaatgcagc
     4861 cggaatggac tttaatatga tttatttcaa cgctgatgga aatgaaagct cgatgtgtgg
     4921 caatggcggc agatgcctgg taagttttgc caatgacctc ggcattattc aacaaaatgc
     4981 tgcatttaat gccatagacg gagcacatga tgctgtatat ttttcacctc agaaagtgaa
     5041 tctgcgcatg aaagatgtca gtgaaataca aaaaggaaat ggtttcgctg ttctggacac
     5101 cgggtcgcct cattacgttc ggtttgtgga tgatctgagt gttgtgaatg tgtacgaaga
     5161 aggcaggcaa atacggtata gtcctgaatt tgcggaaaat ggcatcaatg taaatttcgt
     5221 acacaaaaca gaggacattc ttcacgttta tacctacgag cgcggcgtag aagacgagac
     5281 cttagcttgt ggcaccggtg tgacggcagc agctattgcc tggaatgaag tgttcgggca
     5341 caaatctgaa gatagtgtac caatcatggc aaaaggtggc gatttgacag tcagttttcg
     5401 gaaggtgggc aatacctata ccgacatctg gctttccggt cctgctgtga aggtttttga
     5461 aggggtgatt gatctaaacc atattccgca ctagaccctt tactgcctat aattattact
     5521 ccatacccat ctcaaaagct caaaaaccat agttctctga gcttttatca ctttattttg
     5581 gacattttgt atgaatcatt ttcgttcaaa ttcctaatgc attatttggg ttgaataagg
     5641 cagcatattg aaaatagcga attagctgaa atgtattttt gttgtaacaa ctctgtaaca
     5701 acgtgtcatc aactttgcgg tatgaaaagc aaatatctta atcaaacaaa atttatgaca
     5761 cacaaaaaat tctcattact aaacattttg ctcttgcatc ttatcttca
//
LOCUS       DAOQQY010000181         5771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6093_length_5771_cov_5.438943, whole genome shotgun sequence.
ACCESSION   DAOQQY010000181 DAOQQY010000000
VERSION     DAOQQY010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6093_length_5771_cov_5.438943"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="PLE23_08925"
     CDS             complement(<1..453)
                     /locus_tag="PLE23_08925"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HMX83426.1"
                     /translation="MENQPLINKPKDQTENELQALLFKYLRYWPFILLSVILSAFLAW
                     LYLRYTVPMYEVVSTILIKDDKKGGGGGLSEENVFSDLSMFSSNRNIENEIQILKSRM
                     LMQEVVNKLGLNFSYYTVGRIKTSEAYKSSPVQLNADSLTKEAYGKVFD"
     gene            complement(462..1232)
                     /locus_tag="PLE23_08930"
     CDS             complement(462..1232)
                     /locus_tag="PLE23_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HMX83427.1"
                     /translation="MNTKLLPLLSLLLLTACVSNKELTNFQSGENWDSVPKEIPQQPA
                     LVIQPDDVLSITVHSFDMAAAAPYNTDFNLTAAQGSVASIATPGGYLVGPDGYIDFPV
                     IGRIMLGGLTVEQAKNTVQKLVAKQLVDPVVMVRFLNFRITVIGEVSNPATFTIPNQK
                     INILEAIGLAGDLTPYGKRDNILVIREQNGKREFGHINLKDKSIFSSPFFYLAQNDVI
                     YIEPIKEKANTISERSTKILPWLSIAVTTLTLLATLLK"
     gene            complement(1793..2764)
                     /locus_tag="PLE23_08935"
     CDS             complement(1793..2764)
                     /locus_tag="PLE23_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HMX83428.1"
                     /translation="MPKKVHNKHDKFVRASFSNAERAAAFFESFLPQKLAAELDLQLL
                     KANQESYIQDDLSEYFSDLVFEVPTKGGNPVDLVLLFEHKSAPDKHVLIQIGHYMFSH
                     WFKSLSNKQPLKPIIPVIYYQGKQQWDDPQIRPLFGELADTISSYLPELRHIFIALNT
                     LSDQSIEQIRERLMAAAVLAQKKGINTIKLAEDLLRIFQLLPNHSEEGNFLDQLFVYI
                     AYTSEISSETMEKAIEQLPNKVRHNAKSTYAQFVAHGKEEGIQEGIQKGKIEGRIEGR
                     IEGKIEGKIEVILTGFDNGLDISFISIITHLSAVEVTEILKKHGKIN"
     gene            complement(3235..3960)
                     /locus_tag="PLE23_08940"
     CDS             complement(3235..3960)
                     /locus_tag="PLE23_08940"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83429.1"
                     /translation="MMFGLFKKQVPEKHAGHHLLTDMHSHLLPGIDDGAPDIATSISL
                     IKGLAALGYTWLITTPHIMNDMYPNTPHIINERLHDLRQALSKQNIDIQIEAAAEYYV
                     DEFFEELLQTEELICLPGNKILVEQSMLQAYPRFKDVVYELRLKGYTPIIAHPERYVY
                     MAEQKHDYQWFKDSGCLLQVNLLSLQGYYGKSERKLAEYLYREGMIDLYGTDCHHDRH
                     LARLSKFATETNAIRLSENTKIY"
     gene            complement(3988..4998)
                     /locus_tag="PLE23_08945"
     CDS             complement(3988..4998)
                     /locus_tag="PLE23_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMX83430.1"
                     /translation="MTKIKFTIIGLGHIGKRHAEMVQRHPEAEVVALVDLQDPATLNI
                     ARLGMPVFDSIDAFLASGIDTDVVNICTPNGLHTTHAIQALDNKKHVVIEKPMSLCKS
                     DAEKVLYKALNVHRQVFCVMQNRYSPPSVWIKNLIESGTLGKIYIVQLNCYWNRDNRY
                     YKPGNWHGTKDLDGGTLFTQFSHFIDIMYWLFGDITNIRTRLADHNHADLTEFEDSGF
                     VSFDFINGGMGCLNFSTSVWDKNLESSMTIIAEHGSVKIGGQYMDKVEYCHVKDYTMP
                     ELAPTNPGNDYGAYKGSAQNHNYIIDNVIDVLKNRATITTNALEGLKVVEIIERIYQA
                     AE"
     gene            complement(5184..5729)
                     /locus_tag="PLE23_08950"
     CDS             complement(5184..5729)
                     /locus_tag="PLE23_08950"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HMX83431.1"
                     /translation="MQVAQFRSISEQLPIADKQWYAIRTLLNCEKKVAGFLSEISVEH
                     YLPLQQYTRIYGRRKANVTKPLIRNYLFVKITRRDYIKILDTQFVLGFVKFGDQINAI
                     PEREIDFIRLIVGEYQDFEVEQSEIAVGEEIEIIGGLLTGLKARMTERKGRNKVLVEL
                     QSVGVGLLLDVEEKYIRRIVS"
BASE COUNT         1381 a         1230 c         1328 g         1832 t
ORIGIN      
        1 atcgaatact tttccgtaag cttccttagt gagactgtcg gcgttcaatt gtacaggtga
       61 gcttttgtat gcttcacttg tcttgatgcg gcctacggtg tagtagctga agttgaggcc
      121 aagtttgttg accacttcct gcatcagcat acgcgacttg agtatctgta tttcgttttc
      181 gatgttgcgg ttagacgaga acatcgacag gtcggagaac acgttttctt ccgagaggcc
      241 gccaccacca ccttttttgt catctttgat gagtatggtt gacactactt cgtacatagg
      301 taccgtatag cggaggtaca accaggccag gaaagccgat agaatgactg acaggagaat
      361 gaaaggccag taacgcagat atttgaacag caaggcctgc agttcgtttt cggtctggtc
      421 ttttggttta ttgatcagtg gctgattttc caaaacaatg attatttaag caatgtagct
      481 aatagggtta aagtagtgac tgcaatagac agccaaggca gtatttttgt gctccgctca
      541 ctaatggtat tcgccttttc tttgatgggt tctatgtaga tgacgtcatt ttgtgccaag
      601 tagaagaacg gagatgaaaa aatacttttg tctttaaggt ttatatgccc gaactcgcgt
      661 ttgccgttct gctcacgtat caccaatatg ttgtcgcgtt tgccataagg tgtgaggtca
      721 ccggccaggc caatggcttc gagtatgttt atcttttggt taggtatggt gaaggtagca
      781 gggttgctca cctcacctat gacagtaatc cggaaattga gaaagcgcac catcaccacc
      841 gggtccacaa gctgtttggc tacaagtttt tgtacggtat ttttcgcttg ttctacagta
      901 aggccaccca gcataatccg cccgataacc gggaagtcta tgtagccatc cggcccgaca
      961 agatatccgc ccggtgtagc aatactcgct accgagccct gtgctgctgt taggttaaag
     1021 tccgtattgt aaggtgcggc agcagccata tcgaagctgt gtaccgttat ggacaataca
     1081 tcgtccggct ggatgacgag tgccggctgc tgaggtattt cttttggtac ggagtcccag
     1141 ttctcacccg actggaagtt ggtcaactct ttattggaga cacaagctgt cagcagaagt
     1201 aacgagagca gtggcaataa ttttgtgttc attatcaact tagttggtta attgtagtaa
     1261 taattatgta tttggcattt gggcgtgccc ctgtcgggtc gggctatccg gcttacgttg
     1321 tgccgccttc tatccctcac gcattcaatt aaaaattaaa agtgaaaaat taaaaattaa
     1381 aagtgcgaag tgagtggtgt gtttgatttt ttgtacaact ttccgtttgt cccagcctct
     1441 ctttgtctga atcaggattc gcttgatttt tgacaaaaat gtgtagcaaa aagatttatg
     1501 attttccttt cattcaaaat cttcgttttg ctccctttgg ggtgagaggc aaacaaaacg
     1561 gaaattttta tacaactctc cacttgcccc ggcacagtat ccgcttaatt ctcttaactc
     1621 cttaatggtt caaaataaac cgatacaata atgtgtcaca acatctcact tttcacttgt
     1681 aattcgtaat tcgtaattcg taattgaaac attaaatccg tgcaacaaat gattctgaca
     1741 aaaagtattg atagccggat gagatagaat gcaattctgt cgggttttga tattaattaa
     1801 tcttaccgtg tttcttcaat atttctgtaa cctccacagc gcttaaatgc gtaattatgc
     1861 ttataaatga tatatccaaa ccattgtcaa agccggtcag gattacttca atcttccctt
     1921 caatcttccc ttcaatcctc ccttcaatcc tcccttcaat cttccctttc tgaatgcctt
     1981 cctgaatgcc ttcttctttg ccatgagcta caaactgggc gtaggtcgat ttggcgttat
     2041 gtctaacttt gtttggtaat tgttctatcg ctttttccat agtttcgctg gatatttctg
     2101 aagtatatgc tatataaaca aataactgat ccaaaaagtt tccttcctcc gaatggttag
     2161 gcagaagttg aaatatccgt aggaggtctt ctgctagttt tatggtgttg atccctttct
     2221 tttgtgccag taccgccgct gccatcagcc tttctctgat ttgttctatg ctctggtcag
     2281 atagtgtgtt cagcgctatg aagatgtgcc ggagttcggg taggtaactg cttatggtgt
     2341 ctgccagttc gccaaataat ggccgaattt gtgggtcatc ccattgctgc ttgccctgat
     2401 agtaaatgac cggtatgatg ggctttagtg gctgcttgtt gctgaggctt ttgaaccagt
     2461 gtgagaacat atagtgccca atctggatga gtacatgctt gtcgggtgct gacttatgct
     2521 caaacaacag cactagatct accggatttc cgcctttcgt cggtacttcg aaaacaaggt
     2581 ccgaaaaata ctcgctcaag tcgtcctgga tgtaggattc ctggtttgcc ttcagaagtt
     2641 gcaggtcgag ctctgctgcc agcttttgtg gcaggaagct ctcgaagaag gccgctgccc
     2701 gttctgcatt tgagaacgat gctcttacga atttgtcgtg cttgttgtgg actttctttg
     2761 gcatgaaagc ttagtatgat ggtcgtggtt tggtattttt agattattgc tatgttgtgg
     2821 tagtccatcc aaccgctctg gctatgctga cctcctgccg ttttttaatg gtgaatggta
     2881 aatggtacga atttcatcca aaatcttcgt tttgctccct ttggggtgag aggcagacaa
     2941 aacggaaatt tttatacaac tctccacttg ccccggtgca gtatccgctt aattctctta
     3001 acttcttaat ggttcaaaat aaaccgaaaa aaaacgtgtc acaacatctc actcttcact
     3061 tttcactctt cacttttaat ttttaatttt tagttgaatg cgcgtgaagg atagaagccg
     3121 gcacgaagta aggcggatag cctgacccga caggggcacg ccctaactat atagcatgtt
     3181 acgtaccatg tattgaacct gcacatgtat gaatatatct gtgtaccaat gtgtttagta
     3241 tatcttggtg ttttctgaca accgaatggc attggtctca gttgcgaact tgctgagtct
     3301 ggccaaatgg cggtcgtgat ggcagtcggt accgtacagg tcgatcatgc cttcgcggta
     3361 taggtattcg gcgagtttgc gttcggactt gccataataa ccctgcagtg agagcagatt
     3421 gacctgaagt aggcagcctg aatctttgaa ccattggtaa tcgtgctttt gttcggccat
     3481 ataaacgtag cgctccgggt gtgcaatgat gggtgtatag cctttgaggc gcaactcgta
     3541 cactacatct ttgaagcgtg ggtatgcctg gagcatactt tgttcgacaa gtattttgtt
     3601 gccgggcaga caaatgagtt cttcggtctg aagtaactct tcaaaaaatt catcaacata
     3661 atattcggca gcggcttcta tttggatatc aatgttttgc ttactgagtg cttgcctgag
     3721 atcatgcagc ctttcgttga tgatgtgtgg tgtgttgggg tacatgtcgt tcatgatgtg
     3781 gggcgtggtg atgagccagg tataacctag tgctgcaagc cctttgatga gcgagatgct
     3841 ggtggctatg tctggtgcac cgtcatctat acccggcagg aggtgcgagt gcatatctgt
     3901 gagcagatga tgtcctgcat gtttttcggg tacttgtttc ttgaataaac caaacatcat
     3961 tatgtgctgt acgttgtata atttgtatta ttcggccgcc tgatatatgc gttctattat
     4021 ctccactacc ttgagtcctt caagtgcatt ggtggtgatg gtggcacggt ttttcagtac
     4081 atcgatgaca ttgtcgatga tgtagttgtg gttttgggca gaacctttgt atgctccata
     4141 gtcgtttccg gggttggtag gtgccagttc aggcattgta taatctttca catgacaata
     4201 ctctaccttg tccatgtatt gaccgccgat ttttacagaa ccatgttctg ctatgatggt
     4261 catactgctc tcgaggttct tgtcccacac ggatgtagag aaattgaggc aacccatgcc
     4321 accattgatg aaatcaaaac tgacaaaacc actgtcttcg aattcggtaa ggtcggcatg
     4381 gttgtggtcg gccaggcgag ttcggatgtt ggtgatatcg ccgaacagcc agtacatgat
     4441 atcgataaaa tgtgagaact gtgtgaacag cgtaccgcca tcgaggtctt tggtaccgtg
     4501 ccagttgccg ggtttgtagt agcggttgtc gcggttccag taacagttga gctgtacgat
     4561 gtatattttg ccgagtgtac ctgactcgat gagatttttg atccacaccg aaggtggcga
     4621 ataacggttc tgcatgacac agaatacctg tcggtgaaca ttcagtgcct tgtagagtac
     4681 cttttcggca tcactcttgc acaggctcat cggcttttcg atgacaacat gctttttgtt
     4741 gtcgagcgct tggatggcgt gcgtggtatg aaggccgtta ggtgtacaga tattgactac
     4801 atcggtgtca atgcctgagg ccagaaatgc atcgatactg tcgaatacag gcataccgag
     4861 ccttgcaata ttgagtgttg cagggtcttg caggtccacc agtgctacaa cttcagcttc
     4921 aggatggcgc tgcaccattt cagcatgcct tttgccgatg tggcccaagc caatgatggt
     4981 aaacttaatt tttgtcatga ttgcttttga ttgaatacgg cttcgccgtc cttaagtaac
     5041 agtgtactta tggtaaactt cataatttcg gatgtgtctg cgtccggtag aggatttatc
     5101 atcctaatac agagcattat tttcactaac actataattc tgttcaggca atcttttttg
     5161 accgcctgaa aactcatttt cctttaacta acaatacgcc tgatgtattt ctcttctaca
     5221 tccagcaaca aaccaacacc aactgactgc agttctacca gtactttgtt ccgtcctttg
     5281 cgttctgtca ttctggcttt caagcctgtg agcagtccgc ctattatttc tatttcttcg
     5341 cctacggcta tttcggattg ttctacttca aaatcctgat attcgccgac tatcaatctg
     5401 ataaaatcta tctcccgttc ggggatggca ttgatctggt cgccaaactt aacgaatccg
     5461 agtacaaact gtgtatccag tatcttaata taatcacggc gtgtaatctt aacaaagagg
     5521 tagttccgga taagaggttt agtgacgttg gcctttcgtc tgccatatat gcgtgtatat
     5581 tgttgtaatg gcagataatg ctctacagat atttcagaaa gaaaaccggc tacttttttc
     5641 tcacagttaa gcaaggtacg tatggcgtac cactgcttgt cggcaatggg caactgttcg
     5701 cttatggatc tgaactgtgc tacctgcatg atgctttatc tttatgctgt ccttaaagtt
     5761 tccagtacgt a
//
LOCUS       DAOQQY010000182         5717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6182_length_5717_cov_6.274991, whole genome shotgun sequence.
ACCESSION   DAOQQY010000182 DAOQQY010000000
VERSION     DAOQQY010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6182_length_5717_cov_6.274991"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="PLE23_08955"
     CDS             complement(<1..389)
                     /locus_tag="PLE23_08955"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase family protein"
                     /protein_id="HMX83432.1"
                     /translation="MTNSNYSRNTNLFKSVFLAIVVIYLTALVSGFESLTFAMKPLLL
                     LPLLLLVVSSKGIVGKTLLVLALCFSWAGDTLLLLVYKSSIFFLLGLAAFMLAHILYI
                     VLFRLKAPYKAISTKWNVALLSLIGLY"
     gene            complement(473..1741)
                     /locus_tag="PLE23_08960"
     CDS             complement(473..1741)
                     /locus_tag="PLE23_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMX83433.1"
                     /translation="MEQYGKILLIAMPVFLVLVLAEKMYGYLKDYDTVRYMDMVSSLS
                     SGITNIVKDVLGLSVTILSYSWLVKHIAVFELKSNLLVFTTAFIALDFQGYWVHRLSH
                     EINIFWNKHAIHHSSEEFNLACALRQSVSSFVNLFAFFLVPAALLGVPAEVIATVAPL
                     HLFAQFWYHTQHIGRMGFLEKIIVTPSHHRVHHAINPEYIDKNHGQIFIIWDKLFGTF
                     QEERDDIRPVYGITRPAKTWNPIRINFQHLVLMLTDALRTKNLNDKLKIWFMPTGWRP
                     KDVDEQFPVPGIEDIFQFDKYNPATTFVLQLWSGVQLISVFVFCLFFFGNLKEIGSPG
                     IFYYGLYVFLAVYAYTELMDGKAMAIWWEALKTLSGFSLLYVFGSVELIQEKLWAGEY
                     VVTGWLVASFLITLYMVRYELKQSTLQPTA"
     gene            1902..3902
                     /locus_tag="PLE23_08965"
     CDS             1902..3902
                     /locus_tag="PLE23_08965"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008670 - 2,4-dienoyl-CoA reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0033543 - fatty acid beta-oxidation,
                     unsaturated, even number, reductase/isomerase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HMX83434.1"
                     /translation="MNYPNVFSPLDLGFVMLRNRIIMGSMHTGLEEKSGGFERMAVFY
                     AERARADAGLIVTGGISPNRLGRLYPFGAKLTTKHEADKHRIITDAVHTEGGKICLQI
                     LHGGRYSYHPFAVAPSALKAPISKFRPWKMWQWTIRSTIRDFGNTAFLAKKAGYDGVE
                     IMGSEGYLINEFIAPRTNKRMDEWGGSFENRIRFPLAIVREIKQRAGDDFLIIFRLSM
                     LDLVEEGSSWDEVVTLAKELEKAGVHIINTGIGWHEARIPTIAATVPNGTFSWVTQML
                     RNAVKIPLVTSNRINTIEQADTILQNGGADLISMARPFLADPEIVKKAKEGTAHLTNT
                     CIACNQACLDNIFSGKTASCLVNPFACNESLMLPSVPVKRKNIAVVGGGVAGLSFALQ
                     AAKRGHSVSVFERQDHIGGQFGLAARIPGKEVYSKTVSYFEENLKLYDCKIYTNTVFS
                     INHIDEHQIDEVVIASGVRPRKINIPGIDHPKVMDYTEAILHPEKVGRKIAIIGAGGI
                     GYDVALLLTENDQINFFDFWGIDKEYLNRGGIKKAVKAESKHTVWLMQRKEGKMGTTL
                     GKTTGWIHRLRLKLAGVKTIPGVNYTKIDDAGLHIVYKNEKQLLPADTIIICAGQESE
                     NDTLKLLTTTSIPVNMIGGAMKAGELDAEKAIREGAELGLHI"
     gene            complement(4102..4178)
                     /locus_tag="PLE23_08970"
     tRNA            complement(4102..4178)
                     /locus_tag="PLE23_08970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4142..4144),aa:Arg,seq:tcg)
     gene            complement(4238..4585)
                     /locus_tag="PLE23_08975"
     CDS             complement(4238..4585)
                     /locus_tag="PLE23_08975"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMX83435.1"
                     /translation="MAFSKSDEFSPDLQNISVFTKALSHPARLSILIELSKQKKTFCG
                     ELVSVLPLAQSTVSQHLKELVKSDLVNVTQMGTNMAYSINKRQLKVLLTQLEDNFSII
                     FKNIKKEKQSKNN"
     gene            complement(4740..>5717)
                     /locus_tag="PLE23_08980"
     CDS             complement(4740..>5717)
                     /locus_tag="PLE23_08980"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83436.1"
                     /translation="SSAGPFSLIIPNVSTADKGFYTLTLLTEKGCTDEQKIYVNIGNN
                     LTPPVLSAENAVLCKGEPLVLQATPFGGQNISYVWYKKNADGSLTVIDSTSWPTLIIS
                     ETNVQNNTEYLVKVDADGCQSTLSNVYLTVLVESGIDANDDLFKLEHNPGTKQFDVLA
                     NDITKGNIVVKFSNIPSNLTVNSIDKGLITIDFPADAQGSYNIDYTVCSELCPELCES
                     ATLSILVDKDSIIDEPSDTICDFIPNVFTPNSDGNNDAFFIPCLETYKINSFSVFNRW
                     GNAVYEKENYANDWKGTYRNNPLPAGTYYYVLTIPELKKNITGFITIIR"
BASE COUNT         1790 a         1137 c         1174 g         1616 t
ORIGIN      
        1 aaatacagac caatcaagct taacaaagcg acattccatt tggtggatat tgccttgtac
       61 ggagccttta acctgaacaa aacaatatat aatatatgtg ccaacatgaa agccgccaga
      121 ccaagcaaaa agaaaatgga gcttttataa acaagtaaca ataatgtatc gcctgcccag
      181 gaaaaacaaa gcgcaagtac aagcagagtt ttaccgacaa tgcccttcga tgacaccaca
      241 agcaacaaca aaggaagcag taataatggt ttcatagcaa aagtcaaact ttcgaagcct
      301 gaaacgagtg ctgtaagata aatgacaaca atggctaaga ataccgattt gaacagattt
      361 gtattgcggc tatagtttga atttgtcatt tttaagattt tagttgtcat tcaatttgac
      421 agagcctaat attgttttag actgtttgta atcaatcgtc atttttttca aactatgcag
      481 ttggttgtaa agtggactgt ttcaattcgt acctcaccat atacaaagta atcaaaaaag
      541 atgctacaag ccagccggtt accacatatt caccagccca taatttttcc tgaatcaatt
      601 caacactacc aaatacgtat aaaagactaa agccggacaa ggtctttagt gcctcccacc
      661 agatagccat agctttacca tccatcaact cggtgtaagc atagactgct aaaaacacat
      721 acagaccata gtaaaatata ccgggtgaac cgatttcctt tagatttcca aaaaagaaca
      781 ggcaaaacac gaacaccgat atcaattgta caccactcca caactgaagt acgaaagtgg
      841 tagcaggatt gtatttgtcg aattgaaaaa tatcctcaat accgggcacc ggaaattgtt
      901 catctacatc ttttggccgc caaccggtag gcataaacca aatttttagt ttgtcattta
      961 ggtttttagt ccttagtgca tcagttaaca tcagcaccaa atgctgaaaa ttgatacgga
     1021 ttggattcca ggtctttgcc ggacgtgtaa tgccatacac agggcgaatg tcatcccgtt
     1081 cttcctgaaa agttccgaat aatttgtccc agataataaa tatctggcca tggttcttgt
     1141 caatgtattc cgggttaatg gcatgatgaa cccggtgatg cgaaggcgta acgataattt
     1201 tttcaagaaa acccatgcgc cctatatgtt gtgtatgata ccagaattgg gcaaacagat
     1261 gcaacggagc cacagtggca atcacttctg caggaacacc caataaggct gccggcacta
     1321 aaaagaaagc aaatagattg acaaatgacg acacactctg acgcagtgca catgccaggt
     1381 taaactcctc actgctatga tgaatggcat gtttgttcca gaaaatatta atctcgtgcg
     1441 aaagcctgtg tacccagtat ccctggaagt caagtgctat aaacgctgtt gtgaagacca
     1501 acagattact ctttagttcg aaaactgcaa tatgctttac aagccaagaa taactgagga
     1561 tagtgacaga aagcccaagg acatctttga caatgttggt gatgcccgag ctcaggctag
     1621 ataccatatc catataccgg acggtatcgt agtctttcag gtatccgtac atcttttctg
     1681 ccagcacaag taccagaaat accggcatgg cgatgagcag tattttaccg tattgctcca
     1741 tatcaaacgg tttttatttc agatacaaaa aacatcaggt taattctgct gcaatttaac
     1801 aggtttttct taattttgga atacgacagc ttgtttacca ttttattaaa tagaaaataa
     1861 agataaacca gcgaaagtgc agtcttcaaa attgttctgt tatgaattat ccaaatgttt
     1921 ttagcccgct agatctgggt tttgtgatgc tacgcaatag aatcattatg ggttctatgc
     1981 ataccggtct tgaagaaaag agtggtggat ttgagcggat ggctgtattc tatgccgaac
     2041 gcgccagggc agatgcaggc ttgattgtga ctggaggaat ttcgcccaac agactcggac
     2101 ggctgtaccc gttcggggca aaacttacaa caaagcacga agcagacaag caccgaatca
     2161 taacggatgc cgtacataca gaaggtggca aaatctgcct acagatacta catggtggca
     2221 ggtacagcta tcatcccttt gcggtagcac cttcagcact taaagctccg atttcaaagt
     2281 tcagaccatg gaaaatgtgg caatggacca tacggtcaac catcagggat tttggcaaca
     2341 cagcatttct tgcaaaaaaa gcaggttatg atggtgtcga aataatgggg tctgaaggtt
     2401 atcttatcaa tgaattcata gctccgcgta caaataaacg tatggatgaa tggggtggta
     2461 gttttgagaa tagaatccgt tttccactcg ccatagtcag ggaaataaaa caacgggccg
     2521 gcgatgattt tctcatcata ttcagactgt caatgcttga tctggtggaa gaaggcagta
     2581 gctgggatga agtggtaaca ctggccaaag agctcgaaaa ggcaggtgtg cacatcatca
     2641 atacaggtat aggctggcac gaagcccgga tacctaccat cgctgctacg gtgccgaatg
     2701 gtacattcag ctgggtgacc caaatgctta ggaacgcggt gaaaatccca ttggtgacat
     2761 ctaatcgtat aaatacgata gagcaagcag atacaatatt acaaaacggt ggtgccgacc
     2821 tgatttctat ggcgcgaccg tttctggccg acccggaaat cgtaaaaaaa gccaaagaag
     2881 gtaccgcgca tctgaccaat acctgcatag catgtaacca agcttgtctg gataatatct
     2941 tcagtggtaa gacagcctca tgtctggtca atccgttcgc ttgcaatgaa agcctgatgt
     3001 tgccctctgt gccggtcaaa aggaagaata ttgcagtggt tggaggcggt gttgcgggtc
     3061 tgtcttttgc attgcaggcg gccaaacgag ggcacagcgt ttcggtattc gaacgtcagg
     3121 accatatcgg tgggcagttt ggtttggcag cccgtattcc gggtaaggaa gtttacagta
     3181 agacagtatc atattttgaa gaaaacctca aattgtacga ttgtaaaata tataccaata
     3241 cagtattttc aattaatcat attgatgagc accagataga tgaagtggtg atagcttcag
     3301 gtgtacgccc tcgaaaaatc aatattccgg gcattgatca tccaaaagtg atggattata
     3361 ctgaggcaat cttacatccg gagaaggtag gcagaaagat cgcaatcatt ggggctggtg
     3421 gtattggcta cgatgtggct ttactcctga cggaaaatga ccagattaac tttttcgatt
     3481 tttggggaat agacaaagaa tatcttaacc gtggtggtat caaaaaagca gtaaaagccg
     3541 aatcgaagca tactgtttgg ctgatgcaac gcaaggaagg taaaatgggt accacgctgg
     3601 gaaagacaac cggctggatc cacaggctaa ggctcaaact tgccggcgtt aaaaccattc
     3661 ctggagtaaa ttacacaaag atagatgatg ccggcctaca catagtgtac aaaaatgaaa
     3721 aacaattgct acctgccgat acaatcatca tctgtgcagg acaggaatca gaaaatgata
     3781 cgctcaagtt gctaactaca acaagcatac ccgtgaatat gattggagga gcaatgaaag
     3841 ccggagaact cgatgctgaa aaagcaatca gagaaggtgc cgaactcgga cttcatatct
     3901 gaaaatttaa ctcagattcc gcactaagca ctttactgcc aataattatc agctcaaaac
     3961 catttcaaaa gctcaaaaac catagttcgc tatgctttta ccactttttt atggattttt
     4021 tgtacgaatc attttcctcc aaatttctaa tgcgctattt gggtataaaa aaaggcaccc
     4081 aattattaat taagtgcctt ttggtgactc cggaaggatt cgaaccctca accctcagag
     4141 ccgaaatctg atattctatc caattgaact acggagcctt gagggcgcaa agataattat
     4201 ttcagatttc aaaaagattt ttatctcaaa aaagtgctta attatttttt gactgcttct
     4261 cctttttgat gtttttgaag ataatggaga aattgtcctc gagttgggta agcaatactt
     4321 tgagctgtct cttgttgatg gaataggcca tattagttcc catttgagtt acatttacca
     4381 gatcactttt tacaagctct tttagatgct gtgatacggt agattgtgcc agaggcaata
     4441 cacttacgag ttcaccacag aatgtttttt tttgttttga caattctata aggatactaa
     4501 gtcttgccgg gtgcgataat gcctttgtaa agacagaaat attctgcagg tctggcgaaa
     4561 attcatcact tttactaaat gccattttat taaattgtat gaatacgata aataattgta
     4621 aatatacaat taataaatga tttaatcaag tgttttctaa aaacaaaggc attttctgcg
     4681 gatatctaca gaaaatggcc ttcattttta ttggattttt cgtttcacta atatgggtat
     4741 tatctaatga tggtaataaa ccctgttata ttctttttca attcaggaat ggtcaataca
     4801 taataataag ttcctgccgg tagcggattg ttgcggtaag tgcccttcca atcattggca
     4861 taattttctt tttcataaac tgcatttccc catcggttaa atacgctgaa tgaatttatc
     4921 ttgtatgttt cgagacacgg tatgaagaat gcgtcattgt tgccatcact gtttggcgta
     4981 aacacatttg gaataaaatc gcagatggtg tcacttggct catcaataat cgaatcttta
     5041 tccacgagaa tgctcaaagt ggcagattcg caaagttcag gacacaactc actgcacact
     5101 gtataatcaa tattatagct tccttgtgca tcagcaggga agtcaatggt gataagcccc
     5161 ttgtcaatac tgttgacagt aagattggaa ggaatatttg aaaatttgac aactatgttt
     5221 ccttttgtaa tatcgtttgc taatacatca aattgttttg tacctgggtt gtgctcaagc
     5281 ttgaaaagat catcgttagc atctattccg ctttcgacca atactgttaa atatacattt
     5341 gacaatgttg actggcatcc gtcagcgtcc acttttacaa ggtactctgt attgttctgt
     5401 acatttgttt cactgattat cagtgttggc cagcttgtag aatcaataac tgtaagactt
     5461 ccatcagcat tctttttata ccatacataa ctaatgtttt ggccaccaaa tggagttgct
     5521 tgcaatacca atggctcacc tttgcacaag acagcatttt cggcagacag tacaggtggt
     5581 gtaagattat taccaatgtt tacataaatt ttttgctcat ctgtacatcc cttttcggtc
     5641 agtaatgtaa gtgtatagaa tcccttgtct gcagtactta cattcggaat aatgagacta
     5701 aatggccctg cagatga
//
LOCUS       DAOQQY010000183         5708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6196_length_5708_cov_5.023527, whole genome shotgun sequence.
ACCESSION   DAOQQY010000183 DAOQQY010000000
VERSION     DAOQQY010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6196_length_5708_cov_5.023527"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="PLE23_08985"
     CDS             <1..382
                     /locus_tag="PLE23_08985"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HMX83437.1"
                     /translation="EISFTIENAEIRSFEAPAFKEFDLLAGPMTSSMMQIINGQTSSK
                     QTYTYILAPKNPGKYYIEPAFANLKDGTVLETQPIEINVKSNPEGIKQERKQRNGGFN
                     DFFSTPIKPKATQPAKPERKTYKL"
     gene            382..2109
                     /locus_tag="PLE23_08990"
     CDS             382..2109
                     /locus_tag="PLE23_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HMX83438.1"
                     /translation="MKHRILLYLLLLLATSGFSQSGKVSFFATAEPATIQTGESFTLS
                     FTLSNTSGSDFIPPDLHDFTVINGPNRSVSVQSINGAWKQVTEYSFTLMPKRAGHFNI
                     GPATIVTKKGSLETQPVDVVVNDASGIASMPQKGLKKADGKKDFFVEVVTDKSSAYTG
                     EQVLVDFVFYTRVSVQDFRIMEEPTFGGAYAQELQYYEEPQSQVDINGTGFIRKHIKR
                     YAVFPQESGKTEIQPMTLEVNVFTDTNDPFSGFFRRETETKYFTTEKSFFKVETLPDG
                     APKNFLGAVGNWNLELSPVSTHISKNDALTLLLTVTGDGDIKNVQMPNMKLSYDFEMY
                     APKTVEENTSEKNGRLVSKKVFEIMLLPKAEGTFSIPAEYSWFDTTKKHFESKMFGPF
                     SIVVNKAIPHESTEDDQSTDSTSAPVPALKKYLAVFAGIVILGTLIWSIFFIRAKKKT
                     TQNVVIKDSNQVTIPTTFKQSVAIADPVKGLQVYLNNADQEKFYSQLQKNLTTFLIQK
                     LQIQNPNASQGEIISHLEKTGHDTVMISKIKTIFTNCEQVLYAGFDKSAMMLLMVEHL
                     EDIKNRLNT"
     gene            2181..3959
                     /locus_tag="PLE23_08995"
     CDS             2181..3959
                     /locus_tag="PLE23_08995"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83439.1"
                     /translation="MRLVLLFMSLIFIGTLSAQNIDKRKEALKEIIREKLHTARFKQN
                     FEKHAPFIIQKRTNQEIKLSNATSLDVTEAEPYIAINPTDTNNIVVSFMDFTQSLDFR
                     IYYTLDGGATWQLSSFDPRTIYTQRNPGLQIAGGGDPIMDFDKNGKLYYGWLYLGLQG
                     FTEGKFQVFWAWSDDKGATFNTAPGDNIYIAQGGLSLLTGDVTDYGDGIFDRPWFSVD
                     KTDGPNAGTLYCAGLFIPNTKTTLAGNGIIVKRKLPGVDSFEYANTPVFVNENSQFSN
                     IEVDDNGVVHVTYGAIDSSTIMHSRSLDGGLTFEPPTIVGTGKSTFQSGTVYVHAREN
                     PAPNLAREAGTNNLYLTWSSFGEKDLVQGFFAYSKDGGVTWSTPVNLSETAKDTGSHC
                     MMPVVAANTNSGVTVSWYSVTPQKIGNYYVMRSTDGGLSFKNPVLVSSKATDFSVYPT
                     GGFSPVFFGDYNKSINTDCRNLTIFSDGRDNLGPKIYISVVDYCNLTSIPELSSITDK
                     ISFRIQPNPVSSRFSLDISCKESTNTRITLIDSEGKYVKNLYNGKLEAGKTNLEFNIG
                     AQIPAGIYFVVFDADNGKFVKKLIKE"
     gene            complement(4114..5064)
                     /locus_tag="PLE23_09000"
     CDS             complement(4114..5064)
                     /locus_tag="PLE23_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HMX83440.1"
                     /translation="MRRILANDGIHPEAQHLLEEAGFEVITQKVPQSELAGVIGNFDA
                     AIVRSATKITADVLADSGKLKILGRAGVGLDNIDTEAAKQAGIEVFNTPSASSKAVAS
                     LVIAMISGIARNLHLAARTMPVKGATHFSELKKQYAGGMELEGKKLGIIGFGRIGQEL
                     GKMAVGLNMEILPYDAYFKPDTLACEFAGKKVKIQLKVLSFEDVISEADIISVHVPAQ
                     KGSALITAEEISKMKDGVILINTARGGIIDETALLAALNSGKVMAAGLDVFVNEPTPD
                     AALLAHPSVFATPHTGAETAEAQKRIGQALAGRIIDFFEM"
     gene            5223..>5708
                     /locus_tag="PLE23_09005"
     CDS             5223..>5708
                     /locus_tag="PLE23_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HMX83441.1"
                     /translation="MSASKKTKSKQSTPKNLKNRIPKILGLSFILLASFLFVAFISYL
                     FTWQADQDKVLRFSWGLLFEENVTAENFLGRFGAIVSNMFFYWGIGLASFVIVYLFAW
                     AGVHLLKDKKPKRPITVLKNSFFIILFSTIYLEFIFSSSSFPWGGAFGQGTVSWLQSF
                     LG"
BASE COUNT         1716 a         1306 c         1113 g         1573 t
ORIGIN      
        1 tgagattagt tttacgatag aaaatgctga aatccgttcg ttcgaagcac ctgcctttaa
       61 ggaatttgat ttgctggcgg gcccaatgac ttcttccatg atgcaaataa tcaacggaca
      121 gacgagttcc aaacaaacct acacctacat actggcaccc aagaatccgg ggaaatatta
      181 catcgaaccg gcatttgcca acctaaaaga tggaacagta ctcgaaaccc aacccatcga
      241 aatcaatgta aagagcaacc cggaaggtat aaagcaggaa cgaaaacaaa ggaatggagg
      301 ctttaacgat tttttttcta ctcctataaa gcccaaagcc acacaacctg ccaaacctga
      361 acgaaaaacc tataaactct gatgaaacac cgcattctgc tgtatttgct tttactgctt
      421 gctacctctg gtttcagcca gtctggtaaa gtaagttttt ttgctactgc cgaaccggct
      481 accatacaga cgggtgagag ttttacttta agctttacgc tcagcaatac cagtggctct
      541 gactttatac cgcccgacct tcacgacttt acagtcatca acggcccaaa caggtctgta
      601 tcggtacaaa gcatcaatgg agcatggaaa caagttaccg aatattcatt tacactcatg
      661 ccaaagcggg cagggcattt taacattggc ccggctacca ttgtcaccaa aaaagggagt
      721 ctcgagaccc aaccggtcga tgtagtggtc aacgatgctt caggtattgc ctccatgcca
      781 caaaaagggc tcaaaaaagc ggacggtaaa aaagatttct ttgtagaagt cgttacagat
      841 aaatcatcag catataccgg cgaacaagtg ttggtggact ttgtatttta cactcgtgta
      901 tctgtccagg atttcagaat aatggaagag ccaacatttg gtggcgccta tgcacaagaa
      961 ttacaatact atgaagagcc tcagtctcag gtagatatca acggaaccgg attcatacgc
     1021 aaacacataa aaagatatgc cgtctttccg caggaatcag gaaagacaga aatacagcca
     1081 atgacactcg aagtcaatgt ttttacagat accaatgatc cgttttcagg atttttcagg
     1141 cgcgaaacgg aaacaaaata tttcactaca gaaaaatctt tctttaaggt agagacttta
     1201 ccggatggcg ctccaaaaaa tttcctgggt gcagtcggca actggaacct cgaactatcg
     1261 cctgtgagta cacatatttc aaaaaacgat gcacttacac ttctgctcac ggttacgggc
     1321 gatggtgaca tcaagaacgt acaaatgccc aacatgaagc tctcttatga ttttgaaatg
     1381 tatgcaccaa aaactgttga agaaaacaca agtgaaaaaa acggtcgtct ggtatcaaaa
     1441 aaagtgttcg aaataatgtt gctgccaaaa gctgaaggca ctttcagtat tccggctgag
     1501 tactcgtggt tcgacacaac caagaaacac ttcgaatcaa agatgtttgg cccattttcc
     1561 attgtagtaa acaaagcaat accacatgaa agcactgaag atgaccaaag cacggacagt
     1621 acaagtgccc ccgtacctgc actcaagaag tatctggccg tatttgcagg catcgtcata
     1681 ctcggaacgc tcatctggtc aattttcttc atacgtgcaa agaaaaaaac tacccaaaat
     1741 gttgttataa aagatagcaa ccaagttacc ataccaacaa catttaaaca gtctgttgcc
     1801 atagctgacc cggtaaaagg tcttcaggtc tatttgaata atgccgatca ggaaaaattc
     1861 tacagccagt tgcagaaaaa tcttacaaca ttccttatac aaaaactaca gatacaaaat
     1921 cctaatgctt cacaaggcga aataatcagt catctcgaga aaacaggaca tgacacggta
     1981 atgatttcga agattaaaac catctttacc aactgcgaac aggttttata cgcgggtttt
     2041 gacaaatcgg ccatgatgct actgatggta gaacatctcg aagacatcaa aaatcgacta
     2101 aatacctaag ttacacgact tggcaagtca gacaggcttg aaatatcctt ttttagtctt
     2161 atgtttgcaa aaaaacgaat atgagattag tattgttatt tatgtcattg atattcatcg
     2221 gtacgctttc cgcacagaat attgataaga gaaaagaagc cctcaaagaa atcatccgtg
     2281 agaaactgca cacagcacgt tttaagcaga attttgaaaa gcatgcaccc ttcatcatcc
     2341 agaaaaggac caaccaggaa atcaagcttt cgaatgctac cagtcttgat gtcacagagg
     2401 cagaacccta cattgccatt aaccctacag acaccaacaa cattgtagta tcattcatgg
     2461 attttaccca gtcgcttgat ttcaggattt actacacact cgatggtggg gctacctggc
     2521 agctgagttc ttttgacccg cgcacaatat atactcagcg caatccaggc ttgcagatag
     2581 ccggcggtgg tgacccgatt atggattttg acaaaaatgg caagctgtac tatggttggc
     2641 tgtatctcgg attacaggga tttactgaag gaaaatttca agtattctgg gcatggtcag
     2701 atgacaaggg cgccacattc aatactgccc caggtgacaa tatctatatt gctcagggcg
     2761 gtctgtcatt gctaacagga gatgttacag attatggaga tggaatattt gaccgcccgt
     2821 ggttttcagt tgataaaaca gatggtccaa atgccggtac actatattgt gcagggcttt
     2881 tcatccctaa caccaaaact acacttgccg gtaacggtat cattgtaaaa agaaaactac
     2941 ccggcgttga ttcattcgaa tatgccaaca cacctgtttt tgtaaatgaa aattcgcagt
     3001 tttctaacat agaagtggat gataacggtg tcgttcatgt cacttatggt gccattgact
     3061 ccagcaccat catgcatagc cgttcactcg atggtggact tactttcgaa ccaccaacca
     3121 tcgtaggtac cggcaaaagc accttccagt ctggcacagt ctatgtacat gcacgtgaaa
     3181 atccagcacc caacctcgcc cgtgaagctg gtacgaacaa tttgtatctt acttggagtt
     3241 catttggcga aaaagacctg gtacagggtt tctttgctta ctctaaagac ggtggcgtca
     3301 cctggtcaac acctgtcaac ttatcagaaa cagccaaaga taccggaagc cattgtatga
     3361 tgcctgtagt agcagctaat accaattcgg gtgttacagt atcgtggtac tctgtcactc
     3421 cgcaaaaaat cggtaattat tatgtaatgc gctctactga tggtggcctt agctttaaga
     3481 atcctgtatt ggtttcttca aaagctaccg acttttcagt ttacccgact ggcggctttt
     3541 cacctgtatt ctttggtgac tacaacaagt ctatcaacac agattgcagg aatcttacca
     3601 tctttagcga cggacgtgat aatctgggcc ctaaaatcta tatctccgtt gtagattatt
     3661 gcaaccttac atccattcct gaactgtctt caataactga taaaatttcc ttcaggatac
     3721 agccaaatcc ggtcagctca cgttttagtc ttgatataag ttgcaaagaa tctaccaaca
     3781 cccgtataac attgattgac agcgaaggta aatatgtcaa aaacctgtac aatggtaagc
     3841 tcgaagcggg caaaacaaat cttgaattca acatcggagc ccaaattcca gcgggtattt
     3901 attttgtggt ttttgatgcc gacaacggca aattcgtaaa aaaactcatt aaagaataag
     3961 tagaactgag ataatattca gttatccaat tttgcatggt gcctgataat aagatcaaaa
     4021 cgcttactgt caggcactct tgttttacgc ttgttctgct tgatcccaga taattcttcc
     4081 ttaggattta tacctgcata atcacggtac aacctacatt tcaaaaaaat ctatgattcg
     4141 gcctgcaaga gcctgtccaa tcctcttttg tgcctctgcg gtttcggctc ctgtatgtgg
     4201 agtggcaaac acgcttggat gagccagcaa cgcagcatca ggtgttggct cgttgacaaa
     4261 cacatccaga cccgcagcca ttaccttgcc actattaagt gccgccaaca gagcagtctc
     4321 gtcaataatg ccacctcgag cagtatttat aagaatgaca ccatccttca ttttgcttat
     4381 ctcttcagca gtaataaggg cactgccctt ctgtgccggt acatgaaccg aaataatatc
     4441 cgcctcgctt atcacatctt caaagctcaa aaccttcagc tgaatcttta cttttttgcc
     4501 tgcaaattcg cacgcaagtg tgtcgggctt aaagtatgca tcataaggca atatctccat
     4561 attgagccct actgccattt taccgagctc ctgcccgatc ctgccaaaac caatgatacc
     4621 aagcttctta ccttcgagct ccattccgcc tgcatattgc tttttaagtt ccgaaaaatg
     4681 ggtggcgccc tttacaggca tagtccgtgc tgccaagtga agattcctcg ctatgcccga
     4741 tatcatggct attaccaaag aagctacggc cttagaagag gcagatggtg tattgaacac
     4801 ctctatacct gcctgcttag cagcttctgt atcaatattg tcaagcccaa cacctgcccg
     4861 gcccaatatt ttcaattttc cggaatcggc cagcacatct gctgttattt ttgtggcact
     4921 ccgtacgatg gccgcatcaa agttgcctat gactccggca agttcgcttt gcggtacttt
     4981 ttgagttatt acctcgaacc cggcttcttc gagcaggtgc tgagcttccg gatgaatgcc
     5041 atcgttggca aggattcttc gcatgatatt tttttatgaa tcaacaaaaa tacgcgtttc
     5101 agaaacagca aaggcaatat aatttaataa tgaatacgtt atttttgttt tatttattta
     5161 atatgaaata attttcttta tttttgcccc ttagaatagt ccacatttga tcccataatt
     5221 atatgtctgc cagcaaaaaa acaaaatcta aacaaagtac acccaaaaat cttaagaaca
     5281 ggattccgaa gattcttggg ctcagtttta tattacttgc ctcgttcctt tttgttgcat
     5341 ttatcagtta tctctttaca tggcaggccg accaagataa ggttttgaga ttttcatggg
     5401 gcttactgtt cgaagaaaat gtcacggccg aaaatttcct cggcagattc ggagctatcg
     5461 tgtctaatat gtttttttac tggggtatcg gtctggcgag tttcgtcatc gtttatctgt
     5521 ttgcctgggc tggggttcat ttattgaaag acaaaaaacc aaaacgaccc atcaccgtat
     5581 taaagaacag cttctttatc atactttttt ctaccatcta cctcgaattc atcttttcaa
     5641 gctcttcttt tccttggggc ggagcattcg gtcaaggcac agtcagttgg cttcagagct
     5701 ttcttggt
//
LOCUS       DAOQQY010000184         5704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6207_length_5704_cov_4.616038, whole genome shotgun sequence.
ACCESSION   DAOQQY010000184 DAOQQY010000000
VERSION     DAOQQY010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6207_length_5704_cov_4.616038"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(315..551)
                     /locus_tag="PLE23_09010"
     CDS             complement(315..551)
                     /locus_tag="PLE23_09010"
                     /inference="COORDINATES: protein motif:HMM:NF039355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HMX83442.1"
                     /translation="MNKFIKNNILTLTGAILGAIGGYLYWHSVGCSSGSCAITSKPLN
                     STLYGAAMGGLLFSSFEEMLKNKKNKTTGTTGVE"
     gene            complement(544..1134)
                     /locus_tag="PLE23_09015"
     CDS             complement(544..1134)
                     /locus_tag="PLE23_09015"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HMX83443.1"
                     /translation="MMKHLFFVVSLMALISCGENLSKTRGEIDKTALLAKGDSIAMIA
                     QKTLVGKLTSAISERGVPGAVAFCNLNAMPLTDSLSMSNNVGIQRLSDKNRNPANTLS
                     SIHDKEAWTKIQKMMADTGIKDKHLLMEDKNSVYYYKAIPLGMPLCLNCHGQKDKDIA
                     PDVFKIISEKYPQDKAIGYEAGQLRGMWKIKMPVNE"
     gene            complement(1152..1391)
                     /locus_tag="PLE23_09020"
     CDS             complement(1152..1391)
                     /locus_tag="PLE23_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83444.1"
                     /translation="MFNLLRNWDFMRVLRLALGLYVIVEGIRMHDTMLITLGGIFTLM
                     PVFNIGCSAGGTCTPRYRERQTFNPNEDISYKEIK"
     gene            complement(1454..2065)
                     /locus_tag="PLE23_09025"
     CDS             complement(1454..2065)
                     /locus_tag="PLE23_09025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMX83445.1"
                     /translation="MKEFWNQRFSQDEYVYGTEPNEYLKEKLSLLPGGDILLPAEGEG
                     RNAVFAAKTGWKVTAFDQSSEGKKKALLLADKHNVSIDYRVHDVENAEFASESFDVVA
                     LIYTHFPESNRRNYHRKLCSYIKKGGTLILEGFSKAHVAFQAVNPKAGGPVDVSLLYD
                     VEALMQDFDEIDIQEACQTETILHEGENHKGSASVVRITGIKK"
     gene            complement(2165..3577)
                     /locus_tag="PLE23_09030"
     CDS             complement(2165..3577)
                     /locus_tag="PLE23_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX83446.1"
                     /translation="MTVEQIYTGCLAQGAYYITSNGEAAIIDPLRETAPYLKRLEKDN
                     VKLKYIFETHFHADFVSGHIDLSRETGAKIIYGPTAKTGFEAVIAEDNQIFELGNVKI
                     KVLHTPGHTLESSCYLLIDENGKEKALFSGDTLFLGDVGRPDLAQKAANMTQDELAGL
                     LYDSLYTKILPLPDDLTVYPAHGAGSACGKNMMKETVDTLGNQKKLNYALNQPDKESF
                     IKAVTDGLLPPPAYFGMNVAMNQKGYNSFENVLSGGLTPLSPDDFEATAEESGALILD
                     TRNDKIFSKAFIPQSVNIGIDGDFAPWVGAMLADVGQPILLVTEVGREEETVTRLSRV
                     GFDKVLGYLKGGFEAWAKAGKETDQVNRITAGEFASRLNVYQDKVIDVRRESEYTAEH
                     VEESYNRPLDAINDWVKDIDPKEHFFLHCAGGYRSMIAASILQARGYRNFIEVEGGFK
                     AIAQTNIPKTDYVCQSKVLN"
     gene            complement(3693..4343)
                     /locus_tag="PLE23_09035"
     CDS             complement(3693..4343)
                     /locus_tag="PLE23_09035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMX83447.1"
                     /translation="METNVRNAMPRIGDMAPDFQAVTTTGDLKFSEYNKGSWVILFSH
                     PADFTPVCTTEMSGFAVESDFFKNHNTKLIGLSIDSIFSHIAWVNAVDEKTGVLFNFP
                     IIADLDMKVANLYGMLQPGESETATVRAVFIIDPEGKIRLIMYYPLNVGRNMDEIKRA
                     LVALQTSDEHKVAMPLNWQPGQQVIVGAPKTLDALKERKADATLDKTDWYLAKKNL"
     gene            complement(4560..5561)
                     /locus_tag="PLE23_09040"
     CDS             complement(4560..5561)
                     /locus_tag="PLE23_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HMX83448.1"
                     /translation="MKLSNIIYIMLLLAATLQGHAQKTKNMTDKNNPLLCNPQTGICE
                     MPGDKTEKKPDTQPVRSEQVLRIVYFTDPICSSCWGIEPQLRKLKLEYGKKLQIEYHM
                     GGLLPDWSYNSGGISKPSDVASHWDEVSLHYDMPIDGDVWIEDPLDSSYPPSIAFKAA
                     ELQDKEKAVVFMRALRELVFMKKKNITRWENIRVAAIEAKLDEAQLKKDYESGKAKAL
                     FEQDLELARKYGVRGFPSVFVTNAAGASEFVYGSRPYSDYQTAIEKLGLNIEKQLYAK
                     DWESLFGEYPSMTAREYSELSMTSRDKTVRLLDELTTQGKLEKTSCKNGDLWKLKQD"
BASE COUNT         1415 a         1297 c         1130 g         1862 t
ORIGIN      
        1 aatgaatgaa aagaccatga tgtctgaaga aattttaaat tcttccatgc cgatgatggg
       61 caccacggta cgttccaagc cagccattcc gcccaccagg atattaacca agataagcag
      121 tgtgaactgg taccggttat atttgagtcc taaacggggc gttgatgaaa gcataattga
      181 ttaaaaacaa agtaaaacta agtttttttt actttattta aagagcagca cattttattt
      241 gctttttggt tggaaataat ttgactgacg gtgattattg gaaggcagct tacctatact
      301 ctttggttaa gtgattattc tactcccgta gttccggttg ttttgttttt tttattcttc
      361 aacatttctt caaatgagga aaacagcaga ccgcccattg ccgcaccgta gagtgtacta
      421 ttcaatggct tcgaagtaat ggcgcaagaa cccgaactgc aacctacgga atgccagtac
      481 aggtatccgc ctattgcacc caggattgct ccggttaaag tcaagatgtt gtttttgata
      541 aacttattca tttacgggca tttttatttt ccacattcct ctcagctgtc cggcttcata
      601 acctatggct ttgtcttgcg gatatttttc tgaaattatt ttgaatacat caggcgcgat
      661 gtctttgtct ttttgaccat gacaattcag acacagcggc ataccaagag gtatagcctt
      721 ataataatat acgctgtttt tgtcctccat gagcagatgt ttgtctttga ttccggtatc
      781 ggccatcatt ttctgtattt tggtccatgc ttctttgtca tgaattgagc tcaatgtatt
      841 agcagggttc cggtttttat cgctgagcct ttggatacca acattgtttg acattgaaag
      901 gctgtccgta aggggcattg cattcagatt gcagaatgct actgcaccag gtactcctcg
      961 ctcgctgatg gcacttgtca gcttgcctac cagtgttttt tgggctatca tagcaatact
     1021 gtcaccttta gcgagcaaag cggttttgtc aatttctcct cttgtttttg acaaattctc
     1081 accacagctt atcaaagcca taagtgaaac tacgaaaaac agatgcttca tcattttaat
     1141 cgtttcgatg gttattttat ttccttgtag ctaatatctt catttgggtt aaatgtctgt
     1201 ctttctctgt atcgcggagt acaagtacca cctgcactgc acccgatgtt gaatacaggc
     1261 atcagggtaa atatcccacc gagtgtgatg agcatggtgt cgtgcattct gatcccttct
     1321 acaatcacat acagacccaa agccagtctc aaaacgcgca tgaaatccca gtttcttaat
     1381 aaattgaaca taagcctttg ttttaaatat tgatcaataa gttcaaaaat aggcaaaagt
     1441 ttgaatctgc gcactatttt ttgataccgg ttatccgtac tacggatgcg ctgcctttat
     1501 ggttttctcc ttcatgcagt attgtttcgg tctggcatgc ttcctgaatg tcaatttcat
     1561 caaaatcctg cattaacgct tccacatcgt acagcaaaga cacatcgacc gggcctcctg
     1621 ctttaggatt cactgcttga aatgccacat gtgccttgct gaaaccttca agaataagcg
     1681 tgccgccttt tttaatgtac gaacagagct ttctgtggta attgcgtctg tttgattccg
     1741 gaaaatgtgt atagatcaaa gccaccacat caaatgattc tgaagcaaat tctgcatttt
     1801 caacatcatg cactctgtag tcgatagata cattgtgttt gtctgcaagc aacaaggcct
     1861 tttttttacc ttcactactt tgatcaaatg ctgttacctt ccatcctgtt tttgctgcaa
     1921 agacagcatt gcgtccttct ccttctgctg gtaataatat gtctcctcca ggtagaaggg
     1981 ataatttttc cttcaggtat tcgttgggtt cggtgccata tacatattca tcctgactga
     2041 agcgttggtt ccagaattct ttcatcacat tttacttaga ataatcattt ttcattaaat
     2101 gaaagtcagc agctgcagat accaaagtac gctgcatgct gccgactaat atttgaagta
     2161 ttaattaatt caaaaccttt gactgacaga catagtccgt ttttggtatg ttagtctgtg
     2221 ctattgcctt aaaaccacct tccacttcta taaaatttct gtaacctctt gcctgtaaaa
     2281 tactggctgc tatcatactt cggtatccac cggcacagtg taggaaaaag tgttccttcg
     2341 ggtcgatgtc cttaacccag tcgttgatgg catcgagtgg tctgttgtac gattcttcta
     2401 cgtgttcggc agtgtattcg ctttcgcgtc gcacatctat gaccttgtct tggtaaacat
     2461 tcagccttga agcaaattcg ccggctgtga ttcggttgac ctggtcggtt tcttttcctg
     2521 ccttcgccca agcttcaaat ccgcccttca gataaccaag taccttgtca aatccgaccc
     2581 ggctgagcct ggtgacagtt tcttcttctc tgcccacttc ggttaccaat aatatcggtt
     2641 ggccaacatc tgcaagcata gcgcccaccc acggtgcaaa atctccgtca atacctatgt
     2701 tgacagattg tggtataaat gccttcgaaa atatcttgtc attcctcgtg tcaagtatca
     2761 gtgctccgct ctcttcggct gtggcttcga aatcatccgg tgaaagtggt gtaagaccac
     2821 ctgacaatac attttcaaaa ctattatatc ccttctggtt catcgctacg ttcattccga
     2881 aatatgcagg tggaggcaac aacccatcgg tgacggcttt gatgaatgat tctttgtcgg
     2941 gttggttaag tgcgtagttc agctttttct ggttgccgag cgtatctacc gtttctttca
     3001 tcatgttttt accacaagct gagccggctc catgagcagg ataaacagtc agatcatcgg
     3061 gcaatggcaa tattttggtg taaaggctat cataaagcaa tcctgcaagc tcgtcttgcg
     3121 tcatgttggc tgctttttgt gccagatcgg ggcggcctac atctccgaga aacaaggtgt
     3181 cgccactgaa cagggccttc tctttgccat tttcatcaat cagcagataa caagagcttt
     3241 caagtgtatg accgggtgtg tgcagaacct ttatcttcac atttccaagc tcaaatatct
     3301 gattgtcttc cgcaatgact gcttcaaaac cggtttttgc agttggtccg tagatgattt
     3361 ttgcaccggt ctctctgctc aggtcaatat gcccgcttac gaaatcggca tggaaatgtg
     3421 tttcgaaaat gtatttcagc tttacattgt ctttttccag cctcttcagg taaggtgcag
     3481 tctcccgaag aggatcgatg atagcagcct caccattcga tgtgatatag tatgcacctt
     3541 gtgccaaaca tcctgtataa atttgttcta ctgtcatttt tattttagtt aagagtgatg
     3601 tgatggtaac tgatacaaag ttggagcttt ttttccgggc ttacagttac ttatgttaca
     3661 catccagcta aatgatttta ttgctagtat ttttacagat tcttcttggc aaggtaccag
     3721 tcggtcttat cgagcgtagc gtcagctttt ctttccttga gggcatcaag cgtttttggt
     3781 gcgcctacta tcacttgctg gcccggttgc caattaagtg gcatggctac cttgtgctcg
     3841 tctgatgtct gaagtgctac gagtgcacgc tttatttcgt ccatatttct tcccacattc
     3901 aacgggtagt acatgatgag cctgatcttg ccttccggat caatgatgaa aaccgctctt
     3961 acagtagctg tctcgctttc acccggctga agcattccgt acaggtttgc taccttcatg
     4021 tcgaggtcag ctattatcgg gaagttaaac agtacacccg tcttttcatc tacagcattc
     4081 acccatgcta tgtgcgaaaa gatactgtca atgctcagac cgataagttt ggtattgtgg
     4141 tttttgaaaa aatcgctctc caccgcaaat ccgctcatct ctgtcgtaca tacaggtgta
     4201 aaatcggcag ggtgtgagaa tagtatcacc cagcttcctt tgttatattc tgaaaatttt
     4261 aggtcgccag tggtggttac tgcctgaaaa tctggtgcca tgtcgccaat tcttggcatt
     4321 gcattcctta cgtttgtctc cataataggt ttattattaa ttgaatgatt aaatcgtcac
     4381 aaagttaata cgccaacttg tttacactaa gatacttatg ttacaattaa gcctttattg
     4441 cttcttataa tcaatattta aatatgataa aaaatactta tgtatgaata ctgtgcttta
     4501 ccggtaactg cttaggcatg tctgagcaaa aacacaggtg attttttaag caattctgtt
     4561 tagtcttgtt tcagcttcca cagatcgccg ttcttgcacg atgttttctc caattttcct
     4621 tgggtggtga gctcgtccag caaccttaca gttttgtctc ttgatgtcat cgatagttct
     4681 gaatattcgc gtgcggtcat agacgggtat tcgccgaaca ggctctccca atctttggca
     4741 tacagctgtt tctctatgtt taaaccaagc ttttcaatag ctgtctgata gtcagaatat
     4801 ggcctcgacc catacacaaa ttcgctcgcg ccggcagcat tggtcacaaa aacagaaggg
     4861 aatcctctta caccatattt gcgggcgagt tcaagatcct gttcgaacaa agcctttgcc
     4921 ttgccgcttt cataatcttt tttcaactgt gcctcatcaa gtttggcttc tatggctgcc
     4981 acacgtatat tttcccaacg ggtaatattc tttttcttca taaaaaccaa ctcgcgcaat
     5041 gctctcatga acactacggc tttctccttg tcttgaagct ctgccgcctt aaacgcaatg
     5101 gaaggcggat atgaagaatc aagcgggtct tcaatccata catcgccgtc tataggcata
     5161 tcgtagtgca ggcttacctc atcccaatgc gaggccacat ccgacggctt gctgatgccg
     5221 ccactgttgt agctccagtc gggcaatagt cctcccatgt ggtattctat ctgcagtttt
     5281 ttgccatatt cgagctttag tttccgcagt tgaggctcta taccccagca agacgaacag
     5341 attgggtctg taaaataaac aatgcgcaac acttgttcgc tgcgaacagg ttgtgtatcc
     5401 ggtttttttt cggttttgtc tccgggcatt tcacaaatac cggtctgcgg attgcacaac
     5461 agtggattat tcttgtctgt catattcttg gttttttgtg catgcccctg caaggtagca
     5521 gcaagcaaaa gcatgatgta aatgatattg cttaatttca tccttatgtt aaatatttag
     5581 aaaattatat actacgattg atactctaac aacaccgcat cgtttttgtt cagacccggc
     5641 tacagcacat caattttcga gataaccaaa ttttccgctg ttgaaatcct ggaatgcctc
     5701 catc
//
LOCUS       DAOQQY010000185         5684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6244_length_5684_cov_5.111920, whole genome shotgun sequence.
ACCESSION   DAOQQY010000185 DAOQQY010000000
VERSION     DAOQQY010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6244_length_5684_cov_5.111920"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="PLE23_09045"
     CDS             <1..455
                     /locus_tag="PLE23_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83449.1"
                     /translation="EAGHTSTESDPRGIEADGNLDGNFPTQSGQSNITVRNITFDIRI
                     APAADSDIANVSNKMQDVIKVRRSALLNVSNAVVKGTGTVIDLIDMKDGKGNANVNST
                     ISLSNALSNSILGSEVNPPTGFPGAAVQYGNTGCAKDVFSWTKYSFKE"
     gene            608..1294
                     /locus_tag="PLE23_09050"
     CDS             608..1294
                     /locus_tag="PLE23_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX83450.1"
                     /translation="MTKILLVDDDRHISEILEFNLRNENFDVVAVFSAEEALRQNISQ
                     FDLILLDVMMGGMSGYKMAEILRKDGIHVPIIFLTAKDTENDMLTAFSVGGDDYISKP
                     FSLKEVIARVKAVLKRTRPATEDNGKQGKLSIGRLTIDFDQKEVVLNGEKISLTKTEF
                     ELLTLLAQNPGSLFSRERIINTIWHETPYITERTVDVHIVRMRKKLGAYSDAITSRTG
                     YGYRFNPDGL"
     gene            1291..2994
                     /locus_tag="PLE23_09055"
     CDS             1291..2994
                     /locus_tag="PLE23_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMX83451.1"
                     /translation="MKLSFRQRLFIYFALLFTVFATGIAVFEHSREKNFKTLILEERL
                     GIYTNIIQTAITDTTTNYESALDKVHSYLPAQLRISVIAQQGNVLYDNTVKDVSKMEN
                     HAGRVEIRTAEINGTGSDIRESATKHMPYFYYARKIQSGFIRVALPDDVQMRQFLKAD
                     NLFLYFLLGLFVISIFIIHSITSQVGASIKRLRDFALSPDRQDIVFGEDELGETGKKI
                     SENYLQLESGRKDLWQVKNKLLQHILISGEGICFFSNNKQVEFYNALFIQYLHQLIDE
                     PHSDVSAVFSDDIFADFLEVLEKSNETTFETQLKKNGKTFSFRANLFDDKSIEVILSD
                     VTKQEKTRQLKVEMTGNIAHELRTPVTSIRAYLETIIRQNLSEEQQLHFITKAYAQSL
                     TLSEMIRDMSVLAKLEEAPQTFGLETIKIEQLLTKLKEESAAELLDKHIHMEWQLPEG
                     LLLKGNTNLVNAVFRNLIENTIRYAGENISINISVYHQDKDYYYFRYYDTGCGIKDEY
                     HLNRLFERFYRVDEGRTRNTGGSGLGLSIVKNAILFHKGSISVKNRKSGGLEFLFQLH
                     K"
     gene            complement(3304..4650)
                     /locus_tag="PLE23_09060"
     CDS             complement(3304..4650)
                     /locus_tag="PLE23_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMX83452.1"
                     /translation="MKQLPPDFQNRISLQLGKEFEDFRSALQAPLPVSIRYNPKKIHA
                     GRTGEPIPWCEYGRYLPERPVFTLDPLLHAGVYYVQEASSMLIEQAFKQISRNTVGAL
                     RVLDLCAAPGGKSTHLLSMISNDSLLVSNEVIQSRVTILSENLIKWGHPNCIVTCLDP
                     KFFARLEGFFDIILTDAPCSGEGLFRKATKSINEWTSENAANCALRQKRILHDVLPAL
                     KPGGYLIYSTCTYNAAENIDQLTQISKEYPVQSIRLPHLTQFGIEELNENGIFAYQCW
                     PHKVKGEGFFMGILQKTDGITTILKAKKKLSPLSNKTIDFTKWLHCAEDLFLFESRPG
                     EISVVPKILQEGAEIILTQLPVKSIGTHFFTQKAKNLIPTHDSALSVHCYPDLPKLEL
                     TLEQALQYLRREHIDTEYPGDGWKIVTYQNQPLGWINATSGRLNNKLPLEYRIRNL"
     gene            complement(4731..>5684)
                     /locus_tag="PLE23_09065"
     CDS             complement(4731..>5684)
                     /locus_tag="PLE23_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HMX83453.1"
                     /translation="LYYEHQKHTGEFPIIGVNTFLSKKGSPTIIPQEVIRSTTEEKEY
                     FRTYYNSPYFFDAKIFKHGYELKNYELQKVYRQDERHFIRLLDNIRTGTFDWDDLEEI
                     NQRVEPDANKDEMIVLAGRNATVDRINKDSLSKIKEETYTFHANVTGNFDLRRCPADI
                     SLDLKKGARVMFIKNDPLRKYVNGTLGIIDSMTFNEIKVRILDNNLNETIVTVEKETW
                     EMHRYKLNQENQSKIDIEVIGTFSQFPVRLAWALTIHKSQGKTFDRLFLDNEQRMFEH
                     GQLYVALSRCRTMDGLHLHKPVTPRDIMIDPTVMSYYVSNF"
BASE COUNT         1664 a         1098 c         1212 g         1710 t
ORIGIN      
        1 gggaggccgg acacaccagc acagagtcag acccacgagg catagaggcc gatggtaatc
       61 ttgatggtaa ttttccgaca caatcagggc aatcgaatat tacggtacga aatataacct
      121 tcgatatcag aattgccccg gcagcagaca gcgatattgc taatgtaagc aataagatgc
      181 aggacgttat caaggtgaga aggtcggctt tgctcaatgt atcaaatgct gttgtaaaag
      241 gaaccggtac agttatagat ttgattgaca tgaaggatgg aaaaggtaat gccaacgtaa
      301 actcaaccat cagtttaagc aacgcactta gcaacagtat cttgggaagt gaagtcaacc
      361 ctccgacagg ttttcccggg gctgctgttc agtatggcaa tacaggatgt gcaaaggatg
      421 tattttcgtg gactaaatat tctttcaaag aatgaaaaat ccggtatttg cttagtgtaa
      481 tgaatgaaga agcgtatcct gcataggtcg cttctttcct ttatttataa aattataaga
      541 tggcccgaaa atgacgtcaa tatcattaag tcctttatct ttgttgaagc acactgattc
      601 tttcgaaatg acaaaaattc ttctggtaga tgatgacaga cacatttcag aaattctaga
      661 gtttaacctc cggaatgaaa actttgatgt agtagccgtt ttttctgcgg aagaagccct
      721 cagacagaat atttctcaat ttgacctcat tttgctcgat gtaatgatgg gtggtatgtc
      781 ggggtataag atggcagaaa tattgagaaa agatggaatc catgtaccga tcatttttct
      841 aacagccaaa gataccgaga acgatatgct caccgctttt tcagtaggag gcgatgatta
      901 catctccaaa ccattttctc tcaaggaagt aatagcaagg gttaaagcag tgttgaagcg
      961 aacccggcca gcaaccgaag ataacggcaa acaggggaag ctaagcattg gcaggcttac
     1021 cattgatttt gaccagaaag aagttgttct gaacggagaa aaaatttcat tgactaagac
     1081 agaatttgag ctactaacat tactcgccca gaaccccggt agcttgtttt caagagaaag
     1141 aatcatcaat accatctggc acgagacacc ttatatcacc gaacgaactg tagatgtgca
     1201 tattgtaagg atgaggaaaa aacttggggc gtatagtgat gctattacaa gtcgcacagg
     1261 ttatggttac aggtttaatc cggatggtct atgaagctta gctttcgtca gcggttgttc
     1321 atatattttg cattgttatt tacagtattt gctacaggca ttgccgtatt cgaacattca
     1381 agggaaaaaa attttaagac attaattctc gaagaaaggc ttggcattta tacaaatatc
     1441 attcaaactg ccataaccga tacaaccacc aattatgaat cagcattaga caaggttcat
     1501 tcatatctgc cagcacagct taggatttct gtcatcgcac agcaaggaaa tgtactgtac
     1561 gacaatactg taaaagatgt aagtaagatg gaaaaccatg ctgggcgagt agaaatacgt
     1621 acagcagaaa tcaatgggac gggtagtgat atcagagaat ctgctactaa gcacatgcct
     1681 tacttttatt atgctaggaa aatacagtca ggtttcatca gagttgcatt gccggatgat
     1741 gtgcagatga ggcaattctt aaaagcagac aatttgttct tatatttttt actcggactt
     1801 tttgttattt caatattcat catccacagc ataacctcac aggtaggcgc atccattaaa
     1861 cggctccgcg attttgcttt gtcgcctgac agacaagata ttgtattcgg agaagacgaa
     1921 cttggagaaa ccggaaagaa gatttcagaa aactatttac aactcgaatc ggggagaaaa
     1981 gacctttggc aggtgaagaa taagttgtta caacatattc tgatttcagg cgaaggtata
     2041 tgtttctttt ccaacaataa acaagtcgaa ttttataatg cactatttat acaatacctt
     2101 catcaattga ttgatgagcc acatagcgat gtaagtgcag tattttctga tgatattttt
     2161 gccgattttc tggaggtact tgaaaagagc aatgaaacca cctttgaaac acaattgaaa
     2221 aagaatggaa aaaccttctc gtttcgtgcc aatctgtttg atgataaaag tattgaggtt
     2281 attctgtctg atgtgaccaa gcaggaaaaa actcgccagc tgaaagtcga aatgacagga
     2341 aatatagctc atgaattacg tacaccggtt acaagcatca gggcttacct cgaaactatc
     2401 atccggcaaa atctttccga agaacaacag ctacatttta tcacaaaggc atacgcccaa
     2461 tcacttacgc tttcagagat gatacgagat atgagtgtgc ttgccaaact cgaagaagct
     2521 ccacaaacat tcggacttga aacaatcaag attgaacaat tactgacaaa gctgaaggaa
     2581 gaatcagctg ccgaactcct ggataagcat atacacatgg aatggcaatt gcccgaaggg
     2641 ctacttctca aaggcaatac aaacctggtg aatgcagtat tcaggaatct gattgaaaat
     2701 actatacggt atgccggcga aaatatttca attaacattt ctgtgtacca tcaggataag
     2761 gattactatt attttcgata ctacgatacc ggttgtggca tcaaagacga gtatcatctc
     2821 aatcggcttt ttgagagatt ctacagagtg gatgaaggcc gtaccagaaa caccggaggt
     2881 agtggccttg gcttatcaat tgtcaaaaat gcgatattgt tccacaaggg aagcatcagc
     2941 gtaaaaaacc gaaaatcggg cggactcgaa tttttatttc aactgcataa atgagatagg
     3001 taagtggtac ttttttattg gatgttttct tgacttaacc ccgtacctcg ccaaaagaca
     3061 ggtgataaac ggactttgga tttatctaat gtaacgcaat agactctttc ctgcaaatag
     3121 atattacttc aaattcattt cataagctca aaaaccatag ttcactttgc ttatatcact
     3181 ttttagggat tttttgtaca aatcattttc gtttaaattc ataatgcgtt acctgggtct
     3241 aatacaatta ttgattaagg tcttattgta gattaactaa atctacagcc gtcttttttc
     3301 cacctacaga ttcctgatcc ggtattcaag aggtaatttg ttattcagtc tgcctgatgt
     3361 ggcatttatc catccaagtg gttgattttg gtatgtgact atcttccatc cgtcgcctgg
     3421 gtattctgta tcgatatgtt ctcgcctcag atattgcaat gcctgttcaa gtgtcagttc
     3481 cagctttggc aaatcaggat agcaatgtac agacaaggca gaatcgtgag tcggaatcaa
     3541 atttttagct ttttgtgtaa aaaaatgtgt gccaatgctt tttacaggta attgtgtcag
     3601 aattatctct gcaccttctt gcaagatttt gggcactaca gaaatctcgc ccggccggct
     3661 ttcgaatagg aataaatctt cagcacaatg gagccactta gtgaagtcaa tggttttatt
     3721 tgataaggga ctgagttttt tctttgcttt gagtattgtg gttatgccat cggttttttg
     3781 gagtattccc ataaagaatc cttcaccttt tactttgtga ggccagcatt ggtatgcgaa
     3841 tatgccattt tcattcagtt cttcgatgcc aaactgagta agatgtggca atctaatact
     3901 ttgtaccggg tattctttgc ttatttgtgt aagttggtct atgttttctg ctgcattgta
     3961 ggtgcaggtc gagtagatca gatatccacc cggcttaagg gcgggcagca catcgtgcag
     4021 tattcttttt tgtctcaatg cacagttggc tgcattttca cttgtccatt catttatcga
     4081 ttttgtagcc ttgcggaata agccttcacc cgagcagggc gcatctgtca gaatgatgtc
     4141 gaaaaatcct tcgagccttg caaaaaactt tgggtcgagg caggtaacta tacagttcgg
     4201 atgtccccat ttaataagat tttcggaaag aatggtaact ctagactgaa ttacttcatt
     4261 gcttaccaaa aggctgtcat tgctaatcat agagagcaaa tgggttgatt tgcccccggg
     4321 tgctgcacac aagtcgagta cgcgcaatgc cccgactgtg tttctgctga tttgtttgaa
     4381 agcctgctct atgagcatag atgaagcctc ttgtacgtaa tacacaccgg catgcaacag
     4441 cggatcgagc gtaaacacgg gtctttcagg cagatatcgg ccgtactcgc accatggtat
     4501 gggttctcct gtgcgcccgg cgtgaatttt cttcggattg tagcggatgc tcaccggtaa
     4561 cggagcctgc agtgctgacc tgaaatcttc aaattccttt ccaagttgta aggaaatgcg
     4621 attttggaag tcaggtggaa gttgtttcat gcgataggat tattcggcgg taaaattagc
     4681 ttatttcatg caaatcacag ttttgattta tagcccgtta gatattcctt tcagaaattg
     4741 gatacataat agctcatgac tgtagggtct atcattatgt cacgcggagt caccggtttg
     4801 tgcaggtgca gaccgtccat ggtgcgacag cggctcagtg ccacatagag ttgtccatgt
     4861 tcaaacatcc tttgttcatt atcaaggaat aaacggtcga aggtctttcc ctggctcttg
     4921 tgaatagtga gtgcccaagc aagccggaca ggaaactggc tgaaagtgcc aataacttca
     4981 atatctattt tcgattggtt ctcttggtta agcttatagc gatgcatttc ccaagtttct
     5041 ttttcaaccg ttacaattgt ttcgtttaag ttgttatcaa gaatacgtac ttttatttcg
     5101 ttgaatgtca tactgtcgat gataccaagt gtaccgttta catatttgcg cagcggatcg
     5161 tttttgatga acatcaccct tgcacctttt ttcaagtcaa gtgaaatatc ggccggacac
     5221 ctacgcaggt cgaaattgcc ggttacatta gcgtgaaatg tataggtttc ttcctttatt
     5281 ttgctgagcg aatccttgtt aatgcggtca acagtagcat ttcggcctgc cagtacgatc
     5341 atttcatctt tgttggcatc aggttctaca cgctggttaa tttcttcgag atcatcccag
     5401 tcgaatgtac ctgtacggat gttgtccagc aagcggataa aatgtctttc gtcctgccgg
     5461 tacactttct gtaattcgta attttttagt tcgtagccgt gtttaaatat tttggcgtca
     5521 aaaaaatacg ggctattgta ataagtgcga aaatattctt tttcttcggt agtagagcga
     5581 attacttctt gtggaataat ggttggtgag ccctttttac taagaaaagt atttacacca
     5641 ataatcggaa actctcctgt gtgcttttga tgctcgtaat acaa
//
LOCUS       DAOQQY010000186         5678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6261_length_5678_cov_5.362440, whole genome shotgun sequence.
ACCESSION   DAOQQY010000186 DAOQQY010000000
VERSION     DAOQQY010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6261_length_5678_cov_5.362440"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            292..654
                     /locus_tag="PLE23_09070"
     CDS             292..654
                     /locus_tag="PLE23_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83454.1"
                     /translation="MGIKRKCKLCKSRFTGRSDKIFCSANCKAIYHQKLKQVTNTATS
                     TIDKILHRNRSILLELMGKTGVSKKIPKSLLDLKRFNYSYITGFHINSRQKTVHYVYD
                     FSWVIFSDMEILIKRLNS"
     gene            complement(1102..1323)
                     /locus_tag="PLE23_09075"
     CDS             complement(1102..1323)
                     /locus_tag="PLE23_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HMX83455.1"
                     /translation="MASTQLRFKTNINCSGCINAVKPHLDNTQGITRWEVDTNVADKI
                     LTLEAEDITEQQVVDIVTKAGYKIEPIQD"
     gene            complement(1415..4033)
                     /locus_tag="PLE23_09080"
     CDS             complement(1415..4033)
                     /locus_tag="PLE23_09080"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMX83456.1"
                     /translation="MKTIQQHTVPVTQLAAELDSDLKLGLSHAEALKRLQTNGPNIIQ
                     TKRKENLFHLLLTQFNNPMVYLLGFAALVSVWFEEYMDAAAIFLVIGINVVIGFWMEL
                     QAEQSMDALKGMTSVATKIIRDGVLTEIPSEEIVQGDVLFFEAGDMVTADARIIQATQ
                     LTVNESALTGESMPVEKNTAELDETTALADLTNMLFNGTFISTGNARAIATATGMHTE
                     LGKIAYLVKISKKSAIPLEKKLQQFTQRFIYISLVIVVLVFIAGMVTNGNSVELLKTA
                     IALAVAAIPEGMPVVATLALAQGMLKMARHKVIVKKLAAVETLGGTTVICTDKTGTLT
                     ENKILLDSVKLADGHSQENHKLLMHIGVLCNNARIITNGDQIREIGDPLEAGLLQYAI
                     THDCCKEDLDSLYPKISELPFSSDTKVMATLHRSSNGFLVYAKGAPEQLLDIATHILN
                     NGSAVPMSDSVRNSWIDKASSLSSSGMKVLAGAYKQSSHADTDFLSNLTFAGLYCFID
                     PIAGGVSKAIHTAHEAGIRVIMITGDHAATAANIAAQLNIIQDNERPLTGAEMPAYEH
                     LTSHDKKLWMNTKVFARVTPAQKLDLVTILQEAGHIVGMTGDGVNDAPALKKADIGIA
                     MGRRGTQVAREAADMVLTDDHFSSIVYAIRQGRTIFSNIQKFVIYLLSCNMSELFVIA
                     VVALAGFQFQLVPLQILFINLITDVLPALALGVSRGDVIIMKHKPRQPDAPLLSRAQW
                     RSVWVYATVISVCTLLPVLVLHYYPLANHPPDGKVYNNLLFFTIILSQLLHVFNMTSG
                     STPLRHSEIVRNKYVWYAIIACLMLTAALFFIPQVRLVLRITQLHTTEWIAILSAALS
                     SVFIIRLFKRTGIIHD"
     gene            complement(4090..>5678)
                     /locus_tag="PLE23_09085"
     CDS             complement(4090..>5678)
                     /locus_tag="PLE23_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HMX83457.1"
                     /translation="EKAKGNTSEAIKKLMGLQPKTVTLIMPDGSEQLTDINKVVTDHV
                     LLVKPGEKIAVDGIVTSGSSYVDESMLTGESLPVGKTEGEKVFAGTINQKGSFRFRAV
                     NVGKDTMLAHIIKMVQDAQGSKAPVQKLVDKIAGIFVPVVIVIAIITFVLWMLLGGDN
                     SLVQGLLAAVSVLVIACPCALGLATPTAIMVGVGKGAEQGILIKDAESLELAKKVNTI
                     VLDKTGTITEGKPQVNAIKWTNENPTAQQVLFSIEKQSEHPLADAVVRYYENTAPVSL
                     DTFESLTGMGAKGAYLGETYFVGNKKLLAENKIEISPELRQRAVEWGEQANTVVWFAD
                     NRHALCVIAISDKVKDTSVEAIQQLQQMGIDVYMLTGDNEATAGAIASQTGIPNFRAE
                     MLPQHKADFIRELQHNGKLVAMVGDGINDSTALATADVSIAMGKGSDIAMDVAKMTII
                     SSDLKKIPQAIRLSKQTVATIRQNLFWAFIYNIIGIPVAAGILYPINGFLLNPMIAGA
                     AMAMSSVSVVSNSLRLKWKK"
BASE COUNT         1438 a         1135 c         1440 g         1665 t
ORIGIN      
        1 gtacttacat gtgtgtaaat ttcggtggtt ttactgctgt tgtgacccaa tagttgtgcc
       61 atcgacggat attatgttgt aaataattaa ttttttcatg atacaatttt attggtcaaa
      121 cttaaggtgt aatacattca tattgtagtg attatgctta tgaaaagatt atttaccgta
      181 atattacgat tatagtgaaa attaatcgta agtttacggt aaatgtatgt ttactgctgt
      241 gtcaagtagg tttggcttca ggataaatct atttcaataa catataccac tatgggcatc
      301 aaaagaaaat gcaaactttg taaaagtcgt tttacgggta ggtcggacaa gattttttgt
      361 tctgctaatt gtaaggccat ttaccaccag aaactcaaac aggtgaccaa tactgccact
      421 tcaaccatcg acaaaatctt gcaccgtaac agatccatcc tgttagaact aatgggaaaa
      481 acaggtgttt cgaaaaagat acccaagtct ttgcttgact tgaagcgttt caactattcg
      541 tacattactg gttttcatat caatagccgg caaaagacag tacattatgt atatgatttc
      601 tcctgggtca tattctcaga tatggagatt ctgatcaaaa ggttgaatag ttgaatggat
      661 gctccgaata tcttactgtc aatgttatgg atgacaaagt aaatagatat gaagcaatag
      721 cgtggtttgt tatttattat ttatttgcaa aatcgatgtt cggatataaa gattggattt
      781 ttgtaattgg gatggcatga ctggaaatgt caatgcacgt atgtggatgg aactgtagat
      841 attcggaatt ggtagttgta atatgggtgt ggttccgata taccggtaat tgtgtgcgcg
      901 tgatggacag aagcgaacac gaagtaaagc ggatggcctg acccggcagg ggcacgccct
      961 tatgaattaa ataatcgcgc tggtatatca ttaatggaaa atgctttgtg ctacgatggc
     1021 ttagcgacag catttcttta tgaagaggaa cctgatgata cgtcttagca gtttcatgtt
     1081 gtgagatgaa attggtaagt gtcaatcctg aatgggttct attttgtagc ctgctttggt
     1141 aactatatca actacttgtt gctctgtgat gtcctcggct tccaaggtca gaattttgtc
     1201 ggctacgttg gtatctacct cccaacgggt gatgccctgg gtgttgtcga ggtgtggttt
     1261 gacagcgttg atgcagccac tacagttgat attggtcttg aatcggagtt gtgttgatgc
     1321 catgatgata ttgaatgttg gataagtaat attaattgta taacagtcga atgcgtggaa
     1381 tggttctgag aatagggaaa agtctgcaca aatgtcagtc gtgtataatg ccggtgcgtt
     1441 tgaacaagcg gatgatgaac actgaactta aagctgcgga taagatggcg atccattcgg
     1501 tggtatgcag ctgtgtaata cggagtacga gccttacctg aggaataaag aataatgcag
     1561 cggtaagcat aaggcaggcg atgatggcat accagacgta tttattgcgc acaatctcgg
     1621 aatggcggag gggagtgctg cccgaagtca tgttgaaaac atgaagtagc tgactgagta
     1681 tgatggtgaa gaacagcagg ttgttataca ctttgccatc gggtgggtgg ttggctagtg
     1741 gatagtaatg taaaaccagc accgggagga gtgtacatac agagatgact gtggcataga
     1801 cccatacaga ccgccactgt gcacggctga gcaatggggc atcgggttgt cttggcttgt
     1861 gtttcataat aatgacatca ccacggctga caccaagggc gagtgcaggc aacacgtcgg
     1921 taataaggtt gatgaaaagt atctggagtg gtacgagctg aaactggaaa ccggccagtg
     1981 ccaccactgc aataacgaag agttcgctca tgttgcagga gagcaagtag atgacgaact
     2041 tctgtatatt gctgaatatt gtgcgccctt gacgtatggc ataaacgata gagctgaagt
     2101 ggtcatcggt gagtaccata tcggctgctt cgcgggctac ctgtgtgcca cggcggccca
     2161 tggcaatacc tatatcggcc tttttgaggg caggagcatc gttgactcca tcgcctgtca
     2221 taccgactat atgtccggct tcctggagta tggtaacgag atcgagtttt tgggccggag
     2281 tgaccctggc gaaaaccttg gtgttcatcc acagcttttt gtcgtgtgat gtgagatgtt
     2341 cgtaagcagg catttcggcg cctgtaagtg ggcgttcgtt atcctgaata atgttcagtt
     2401 gggctgctat gttagctgct gtagccgcgt ggtcgccggt gatcatgatg actctgatgc
     2461 ctgcctcatg tgcagtgtgg atggcttttg atacgccgcc ggctattggg tcgatgaagc
     2521 aatagagacc ggcaaaggta agattgctaa gaaagtctgt atctgcatga cttgattgct
     2581 tataagcgcc tgcaagtact ttcatgccgg aagaagacaa tgatgaggct ttatcaatcc
     2641 aactgttacg tacagaatcg gacattggaa ccgcgctacc attgttcaag atatgtgtag
     2701 cgatgtcgag gagttgttcg ggtgcaccct tggcatagac caggaatccg tttgatgagc
     2761 ggtgaagagt ggccatgact ttggtatctg acgaaaaagg cagttcgctg attttcggat
     2821 agagtgagtc gaggtcttcc ttacaacaat cgtgcgtgat ggcgtattgg agcaagccgg
     2881 cttcaagagg atctcctatt tccctgattt ggtcaccgtt ggtgataatg cgcgcgttgt
     2941 tgcacaaaac gcctatgtgc atgagcagtt tatggttttc ttgagaatgc ccatcggcga
     3001 gcttgacaga atcgagtagt attttgttct cggtaagtgt gccagtcttg tcggtgcaga
     3061 tgactgtggt accaccaagg gtttctactg cagccagctt tttaacgata actttgtggc
     3121 gtgccatctt gagcataccc tgagcaagag cgagcgtggc cactacaggc attccttcgg
     3181 gtatagcagc tacagcgagc gcaatagcgg tcttcagaag ttctacggag ttgccgtttg
     3241 taaccatacc ggcgataaaa accagaacaa caataacgag cgaaatatag ataaaccgtt
     3301 gagtgaattg ctgcaatttt ttttcgagtg gaatggccga tttttttgaa atttttacca
     3361 ggtaagcgat tttacctaat tcggtgtgca tacctgtagc tgtggcgatt gcccgggcat
     3421 tgcctgtact gatgaacgta ccattaaaca acatgttggt aaggtctgcc aaagcagtag
     3481 tttcgtccaa ttcggcagtg tttttttcta cgggcataga ttcgccggtg agtgctgatt
     3541 cgttgacggt aagctgcgtt gcttgtatga ttcgggcatc ggcagtaacc atatcgccgg
     3601 cttcgaaaaa aagtacatcg ccctgtacta tctcttctga gggaatttcg gtaagtacac
     3661 cgtcccttat aatctttgtt gctactgacg tcattccttt caatgcatcc attgactgtt
     3721 cggcctgcaa ctccatccaa aagccgatga ctacgttaat accaatcacc aggaaaatag
     3781 cggcagcatc catatattct tcaaaccaaa cagaaactaa ggctgcgaag ccaagaagat
     3841 ataccatcgg gttattgaac tgtgtaagca acagatggaa gagattttct ttcctttttg
     3901 tctggatgat attcggtcca ttggtctgca gtctttttaa agcttcagca tgtgataaac
     3961 ccagcttcag atcgctgtct aattcagcag cgagttgggt gacaggaacg gtatgttgct
     4021 gaattgtttt cattgttagt aatggtcaaa gttatgaaaa gcaacgacca atacaaagta
     4081 cctgcaggct tattttttcc atttgaggcg gagactgttg gatacgacgc taacgctgct
     4141 catggccata gctgcaccgg ctatcatcgg gttgagcaaa aatccgttga tagggtaaag
     4201 gataccggct gccacaggta tgcctatgat gttgtagatg aatgcccaga agaggttttg
     4261 cctgatggtg gctacagtct gttttgaaag acgtatggcc tgtgggattt tctttaggtc
     4321 ggatgaaatg atggtcatct tggcgacatc catggcgata tcgcttcctt tgcccatggc
     4381 tatgctcaca tctgcagtgg ccaaagcggt actgtcattg atgccgtcac ctaccattgc
     4441 aacaagcttg ccattatgtt gcagttccct gataaagtct gccttatgct gtggaagcat
     4501 ttctgcccgg aaattgggga ttcctgtctg agatgcaatg gcgcctgcgg tggcctcgtt
     4561 gtcacctgtg agcatatata catcaatacc catttgctgc aactgctgga tggcttctac
     4621 ggaagtatcc ttgaccttat cggaaatggc aataacacaa agtgcatggc ggttgtcagc
     4681 aaaccatacc actgtgttgg cctgctcgcc ccattctaca gcccgctgtc ggagttcggg
     4741 agatatttcg attttatttt ctgcaaggag ctttttattt ccgacgaagt aagtttcgcc
     4801 cagataggct ccctttgcac ccattccggt gaggctttca aatgtatcta atgataccgg
     4861 ggctgtattt tcataatatc ggactacggc atctgccagc ggatgttcgg actgtttttc
     4921 gatggagaac aatacttgtt gtgctgtcgg attttcattt gtccatttta ttgcgttcac
     4981 ttggggtttg ccttcagtaa tggtacccgt tttgtcgagg actattgtgt ttactttttt
     5041 ggcgagttcg aggctttcag catctttaat gaggataccc tgttcggcac ctttgccaac
     5101 accgaccatg atggcagtag gtgtggcgag gccaagagcg cacgggcaag cgatgaccag
     5161 aacagagact gcagccaaca atccttgtac cagtgaatta tctcctccca ggagcatcca
     5221 aagtacaaag gtaatgatag caatgacgat aacgacaggg acaaaaatac ctgctatttt
     5281 atctacgagt ttttggacag gtgccttgct tccctgggcg tcctgcacca ttttgatgat
     5341 gtgggccagc atagtatcct tgcctacgtt gacagcacgg aagcgaaaac tacctttctg
     5401 gttgatagta cctgcaaaaa ccttttcgcc ttctgttttg ccaaccggca gagattcgcc
     5461 ggtgagcatg ctttcgtcca cgtaggatga tcccgaagtg acgattccgt caacggctat
     5521 tttttcgccc ggttttacga ggagtacatg atctgtgacg actttgttga tatcggtgag
     5581 ttgttctgag ccgtccggca tgatgagtgt aaccgttttc ggttgcaggc ccatgagttt
     5641 tttaatggcc tctgatgtgt tgcctttggc tttttctt
//
LOCUS       DAOQQY010000187         5663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6285_length_5663_cov_5.803495, whole genome shotgun sequence.
ACCESSION   DAOQQY010000187 DAOQQY010000000
VERSION     DAOQQY010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5663
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6285_length_5663_cov_5.803495"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLE23_09090"
     CDS             complement(<1..445)
                     /locus_tag="PLE23_09090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMX83458.1"
                     /translation="MLSKACEYAIRASIIIASESQQKNRPGLTEIAEKINSPVAFTAK
                     ILQKLVKNKILMSIKGPGGGFEADKNALGTIRLIDIVLAIDGISPFSSCSLGLNECSE
                     HNPCPVHHKYKPIKEKLIEMFDKTTLEELVNAYKAGKTFLKLPDMS"
     gene            835..2190
                     /locus_tag="PLE23_09095"
     CDS             835..2190
                     /locus_tag="PLE23_09095"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMX83459.1"
                     /translation="MNTFIKKSNSWALLVLLFGVIVSSCRKDNDDDLGKNNLVKNTTN
                     TELISSWNREIGKIDYAYGEYRPCPIATVLAYSNFAAYETAVPGMPDYRSLKDVYSGL
                     NLPKYDASKEVNWPLAINAAYGYMYKLYFPKEPALIANQEELMKSKYSTGVSTTVVNN
                     SIEWGQSVAKAVYAYSQSDLVTFDGYLNTTPAYTVKNDPGTWVPTPPDFSPGKFPQWG
                     KGRTLAINEADKQIPAPLAYSTDKNSQYYNQALECYSRVVNGTAEDKWIAEFWSDDLA
                     QLTFSPPVRWLEIANQVYQVSTCNLQKALVTNTKICFGLNDAAIACWDQKFKWQVERP
                     ITFIHKYIDPNFKTILTTDGLTPNFPTYPSGHGTFGSAAAEVLTYEFGTDFGMTDRCH
                     QGRTEFNGTPRSFENFYAMSEENADSRIPLGVHFRMDAKEALKLGYRIGRKVNQLPWG
                     Q"
     gene            2445..4373
                     /locus_tag="PLE23_09100"
     CDS             2445..4373
                     /locus_tag="PLE23_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX83460.1"
                     /translation="MSKKNFSQKPIAPKSGKAAGNTSIAGNKTDRYALILALVVSFLS
                     FLPSLQNEFVNWDDDVNVLENKNIERLDASHIKAIFTDDVIGNYNPLPILTFAIEKHF
                     VEYSPKLYHIDNLILHLLCVLFAFLFVRQLGLSSWASGLVALLFGIHPMRVESVAWIT
                     ERKDVLFTVFYFWALWLYARYLKSGQSNKKLYFLIFPLFILSLLSKIQAVSLPLSMLA
                     VDYLMKRPLKLNLILEKIPHFLLSALFGIIGIYMLGQAKSLDDVTNYSIFGRLAIGGY
                     TFFVYVLKWIFPYKMSPLYAYPDKLSWEIYAAATPTLGLLILTWWLYKKDKREYTFAM
                     LFFIVNVMFMLQILGAGQGFLADRFTYVPYLGLFFGTGYFYDQIRTNQKYKSMVNGFF
                     GLYLLIFIIMTVRQTGIWKNGETLWTHVQKYETQTPLPYSNRAMYYRNNKMYDKALAD
                     FEHALKIKPQGTTYNSMGKLFFDSGDAKKALQYYDKAIEMKPEVGEFYINRGAALATI
                     GNYQKALSDLNTGVSKEPDNLNGYLNRSLLNFTMGNYEAAIQDHNEYLKHKPDKFEMY
                     YERGICKAALKQYESAINDFTTAINAQRRDIFFLERGKAYFMVGKRELAQQDIRTAME
                     LGMKVPPEILQQVGIQNK"
     gene            4395..>5663
                     /locus_tag="PLE23_09105"
     CDS             4395..>5663
                     /locus_tag="PLE23_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMX83461.1"
                     /translation="MKKIVLLLIAGCIWLTTQAQSPSHVPGDILVKVSGAQSIKRIVN
                     DLSALGQKPSQLRAIRQLSVNAGIWLLHFDPKNISEAEMLRSVKAHRDVLEAQYNHYL
                     EQRATTPADADFGQQWQWVNDGTGGGTPDADVDAELAWDITTGGLTPLGDTIVVCVVD
                     DGTTLNHPDLKQNIWVNHGEIPDDGIDNDGNGYIDDYYGWNINSEDDNVGDGNHGTSV
                     SGMIGASGNNGIGVTGINWNVKIMSVRYGSLIESNVIESYDYPLTMRKMYNSSNGTKG
                     AYVVATNSSWGIDNAKSSDFPLWCSFYDTLGVHGILSCASTTNNNANVDIVGDMPTTC
                     TSDYLISVGRTTNKDAVEGGTGIVHVDLGAPGSNIYTTSKNSYKFTTGTSFSSPLVAG
                     IIALMYSAPCADIISLSKTFPGAAALLMKKY"
BASE COUNT         1728 a         1147 c         1131 g         1657 t
ORIGIN      
        1 aactcatgtc gggcaattta agaaaagtct taccggcctt ataagcattg acaagctctt
       61 caagtgtagt tttatcgaac atttcgatta atttttcctt gataggcttg tatttgtggt
      121 gtactgggca aggattatgt tctgagcatt cgttgaggcc aaggctgcaa ctactaaatg
      181 gcgaaatacc atctatggca agcacgatgt ctataaggcg tattgttcca agcgcatttt
      241 tgtctgcttc aaagccacca ccgggccctt tgatggacat cagaatcttg tttttgacaa
      301 gtttctgcag aattttggca gtaaaagcta caggtgaatt tatcttctca gcaatctcag
      361 tcaagccggg tctgttcttc tgttgtgact ctgaggctat tatgatactt gcccgtattg
      421 cgtattcgca tgctttggat aacatttgtc aattttaatt agcacagaat ttgtagtttg
      481 tattacaaaa gtaaaataat cgggcgacaa ttatcaataa aaataataca acataacaaa
      541 cacgatgaag cttttgatga tgattattgg ttttatcaat tgaaataaaa aaaaagtcaa
      601 aaaaatatat atgcgaataa tttgtaactt actagtatct cacataactt tgtgtcgtga
      661 acaggccttg atgagaaaac cggtacaggc tgttacttcg acacaatcgt ttaaacccaa
      721 aaccttttaa tataaaacac cgatttcagg catctggata tgtattctct tgcctgtata
      781 ggataaactt ttattaaata tatttaatta atcaaaccaa atttttattg tatcatgaac
      841 acctttatca aaaaatctaa ctcttgggcg cttttagtgt tgcttttcgg agtcatagtt
      901 tcttcttgtc gtaaagacaa tgacgatgat cttggaaaaa acaatcttgt taaaaacacc
      961 accaacaccg agcttatcag tagctggaat cgtgaaatag gcaaaattga ctatgcttac
     1021 ggtgaatatc gtccatgtcc gattgcaaca gttttggctt atagcaattt tgctgcttac
     1081 gaaacggcag ttccgggcat gcctgactat cgttctctaa aagatgttta ctctggactt
     1141 aatcttccaa aatatgatgc ttctaaggaa gtaaactggc cattggctat caatgctgct
     1201 tatggttata tgtacaaact atatttcccg aaagagcctg ctttaatcgc taatcaggaa
     1261 gaacttatga agtcaaaata ctctaccgga gtatctacta cagtagtaaa taactcaatt
     1321 gaatggggcc agtctgttgc taaagctgta tatgcttatt ctcagtctga ccttgtaaca
     1381 tttgacggat accttaacac tacaccggcc tatactgtaa agaatgatcc tggtacatgg
     1441 gtgcctactc ctccggattt ctctcctggt aaattcccac agtggggtaa aggaagaaca
     1501 ttggctatca atgaagctga taagcaaata cctgcaccgc ttgcttatag cacagacaaa
     1561 aactctcaat attacaatca ggcacttgag tgctactcaa gagtagtaaa cggtactgct
     1621 gaagataagt ggattgctga attctggagt gatgaccttg ctcagttgac tttcagccct
     1681 cctgttcgtt ggttagaaat cgctaaccag gtgtatcagg tttctacttg caaccttcag
     1741 aaagcattgg ttacaaacac caagatttgt tttggtttga atgatgctgc tattgcttgc
     1801 tgggatcaga aatttaaatg gcaggtagaa agaccaatca cttttatcca taagtacatc
     1861 gaccctaact tcaagacaat ccttactacg gatggtctta cacctaactt ccctacttat
     1921 ccttctggcc atggcacttt tggtagtgct gctgctgaag tgcttacgta cgaattcggt
     1981 actgacttcg gtatgacaga cagatgccac caaggtagaa cagaatttaa cggaacgcct
     2041 agatcattcg aaaacttcta cgctatgtct gaagaaaacg cagattcaag aattcctctt
     2101 ggtgtacact tcagaatgga tgctaaagaa gctcttaaac tcggataccg tatcggtaga
     2161 aaagttaacc agcttccttg gggccaataa tagcaccact taaagattgg tgaaaaaata
     2221 tggcaggtcg gttttcggtc tgccatattt ttttacatac aaccactcca acttctttcc
     2281 aattcattca ttagaaaaaa agcagaattg atttaaatcg gctcttcata tcagaaatta
     2341 aacaattgca gataactttc atggaataac tacgtttaga acaagaatgt aactatattt
     2401 gaaaatacat ttgattcctt gttaaagacc gtacattcat tgccatgagc aaaaaaaact
     2461 tttcacaaaa accaatcgca cctaaaagtg gcaaagctgc aggtaacacg tccattgcag
     2521 gaaataaaac agaccgctat gctttgattt tagccttggt ggttagtttt ctaagcttcc
     2581 taccatcact tcaaaacgaa tttgttaact gggacgacga tgtcaatgtt ctggaaaaca
     2641 agaatattga aagattggat gcctctcaca taaaggcaat cttcacggat gacgtgatag
     2701 gcaactacaa ccctcttccg attctcacat ttgccattga gaagcatttt gtagaatatt
     2761 ctccaaaact ttatcacatc gacaatctga tattgcacct gctttgtgtc ttatttgcat
     2821 ttctttttgt caggcaattg ggtctttctt catgggcatc cgggctggtc gcactgttgt
     2881 tcggcattca tcctatgcgg gtagaatcgg tggcgtggat tactgaacga aaagacgttt
     2941 tatttacggt attttacttc tgggccttgt ggctgtatgc cagataccta aagtcgggtc
     3001 agtccaataa aaagttgtac tttttgattt ttcctctgtt catattgtct cttttatcca
     3061 agatacaggc agtgagtttg cctctgagta tgctcgctgt agattatttg atgaaaaggc
     3121 cgctaaaact gaatttgatc ctcgaaaaaa taccacattt cctgttatct gcactctttg
     3181 gtattattgg tatttatatg ctggggcagg caaaatccct tgatgatgtt accaactatt
     3241 ctatctttgg caggctggct attggtggtt atacgttctt tgtttatgtg ttgaaatgga
     3301 tattccccta caaaatgtca cctctttatg cctatccgga caagctttcc tgggaaatat
     3361 atgcggccgc aactcctacg ctcggactct taatacttac gtggtggctt tataaaaaag
     3421 acaagcgaga atatactttt gccatgttgt tctttattgt taatgtgatg tttatgcttc
     3481 aaatactcgg tgccggccag ggttttctcg ctgacaggtt tacttatgta ccttacttgg
     3541 gtttattctt tggtaccgga tatttttatg accagatacg taccaaccag aaatacaaaa
     3601 gcatggtcaa tggttttttt ggattgtatc ttcttatttt catcattatg acagtcaggc
     3661 aaaccggtat atggaaaaat ggcgaaacat tgtggacgca tgtacaaaaa tacgaaaccc
     3721 agacaccgct accatattca aaccgtgcta tgtactaccg caataacaag atgtatgaca
     3781 aggcactggc agatttcgaa catgctctca aaattaaacc ccaaggcacg acatataatt
     3841 cgatgggaaa attatttttt gattcgggcg atgcaaagaa agcactacag tattatgata
     3901 aagctataga aatgaagccg gaagttggtg aattttatat aaaccgaggg gctgcacttg
     3961 ccacaatagg caactatcaa aaagcactaa gcgacctcaa cacaggcgta tcaaaagagc
     4021 ctgacaatct gaatggctat ctcaaccgat ccttgctaaa ctttacgatg ggcaattatg
     4081 aggctgccat acaagaccat aacgaatacc tgaaacacaa gcctgacaaa tttgaaatgt
     4141 attatgagag aggcatctgc aaagctgctt tgaaacaata cgagagtgca ataaacgatt
     4201 ttacgaccgc cattaacgcc caacgccggg atattttctt cctcgaacgt ggcaaagcct
     4261 atttcatggt cggcaaaaga gaacttgcac aacaagacat aagaacagca atggaattgg
     4321 gtatgaaagt tcctccggaa atacttcaac aagttggcat tcaaaataaa taaatataaa
     4381 aattcggcat ccttatgaaa aaaattgttt tactgcttat tgccggctgt atatggctga
     4441 caacacaggc acagtcccct tcgcatgtac cgggtgacat ccttgtaaag gtatcaggcg
     4501 ctcaatctat taaaagaata gtaaatgatt tgtcagcact cgggcaaaag ccatctcaac
     4561 tgagagccat aagacaactt tccgtaaacg ctggcatttg gctcctgcat tttgatccaa
     4621 aaaacatttc agaagctgag atgctaaggt ctgtaaaggc acatcgcgat gtactggaag
     4681 cacagtacaa tcattatctc gaacaaagag ctaccacacc tgctgacgca gactttggtc
     4741 agcaatggca atgggtcaat gatggaaccg gaggaggtac accagatgcg gatgtcgatg
     4801 cggagctcgc ttgggacatt accaccggtg gtttgacacc acttggagac accatagtgg
     4861 tatgtgttgt ggatgacggc accaccctta accatcccga tctcaaacag aatatttggg
     4921 taaaccatgg tgaaatacct gatgatggta ttgataacga cggaaatgga tacatagatg
     4981 actactacgg ttggaacatc aattcagaag atgataatgt aggtgatggc aaccacggaa
     5041 catcagtatc tggtatgatt ggggccagcg gcaacaatgg cattggcgtt accggtatca
     5101 actggaatgt taaaataatg agcgttcggt acggaagcct gatcgaatct aatgtaattg
     5161 aaagttatga ttatcctttg accatgagaa aaatgtataa cagcagcaat ggcactaagg
     5221 gagcatacgt ggtagctacc aactcaagct ggggtattga caatgcaaaa tcatccgact
     5281 ttccgttgtg gtgcagtttc tatgatacac tgggcgtaca tggcatactc agctgcgcct
     5341 ctactaccaa caataatgca aatgtagata ttgttggcga tatgcctacg acctgtacaa
     5401 gcgactacct gatatcagta ggcaggacta ccaacaaaga tgctgtcgaa ggtggtaccg
     5461 gaatcgttca tgttgatttg ggagctcctg gatcaaacat atatactacc tccaaaaaca
     5521 gttataaatt cactacaggt acttcatttt catcaccatt agtagccggt atcattgcac
     5581 tgatgtattc tgctccatgc gccgacatca tttctttaag caaaacattc ccgggagcag
     5641 cagcccttct catgaaaaaa tat
//
LOCUS       DAOQQY010000188         5649 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6309_length_5649_cov_4.885055, whole genome shotgun sequence.
ACCESSION   DAOQQY010000188 DAOQQY010000000
VERSION     DAOQQY010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6309_length_5649_cov_4.885055"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2483
                     /locus_tag="PLE23_09110"
     CDS             <1..2483
                     /locus_tag="PLE23_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX83462.1"
                     /translation="EFRAKQNLPIRLKANETAQQGQNIAVIALPSGNEGDDDDYEVSS
                     LLAASRDVFNSIAGFNFSSAGFDLRGYESEYQSVYINNVPVNDPESGNSIYSTWGGLN
                     EVMRTDRFLNGLSAFSNGFGNIGGTAIIDASAATQRKQKKVSYMFSNRSFQHRLMATV
                     STGTMKNGWAFSASGSHRWANEAYVPGTFLDSWAYYLGAEKKFRNGDQFSVTVFGSPT
                     RRGKAAPSVQEMNDLAGTNYYNPNWGYQNGEKRNARVANTHLPTAIVGYNKKFNAKAQ
                     WNNSLLFQTGREGNSALDWFDAPDPRPDYYRRLPSYAENPDVAAQIAALLKSDEKARQ
                     INWQELYNINDASYGTVENANGISGNNVSGKISKYLISERRTDQTVLSLNSTFSYELS
                     SVAKLSAGVLARSFKGKNFQTALDLLGGDFIVDNDKYAERDFPNDSLALQNDLDVLNK
                     IVKEGEQYGYNYNTHIRSAQPWAQLDITLRKFDIFAAANVGFTNIWREGLVRNGRFPD
                     NSKGNSEKQKFTTYGIKAGTTYKLNGRNYLYANAGYMTQAPLVRDIFQSPRTRNTTIP
                     NLTTEKTLSAEAGYILRAPVLKARVSAYYTQFRDLARTTSFYHDELRTFVNFNLNGIG
                     RTHIGTEIGLDYKISPSFSVQAAANLGDYYYDTRAYADISQDNNNTLVADDRLVYQKD
                     FFVANTPQTAAGLNLRYAGPRSWFATLSASYFDKMYIDFNSDRRTNIAVSGLDDASET
                     YLKIIEQERLDPAYTIDASIGKNWRVKKTFITLNISVNNLLNNTSFKTGGFEQLRFDY
                     ADKDINKFPPKYFYLYGLTYGVNLGVSF"
     gene            2507..4528
                     /locus_tag="PLE23_09115"
     CDS             2507..4528
                     /locus_tag="PLE23_09115"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HMX83463.1"
                     /translation="MIKSIYYIFLSLIMTGLVASCVKHEFDKPPVSTEDPNLTANTTI
                     AELKALHSAGKFDVITDDRIIVGIVIADDESGNYYKTIVIQDQTGGIELKINATDQYV
                     KYPVGRRLFIKCKGLTLGDYNGVVQLGGGVFDNNGSPSLGGIEQALTDTYIFPGQYKQ
                     YVTPKKLKITELTQADVSTLVELEDVEFASGESGKTYADVVGKTTQNRTVEDCNKNVV
                     IMRTSGYANFAGDLLPTGKGTLTAVYSIFGSTKQVYIRDTRDVVFTGTRCGGGSGTVT
                     PNTTIAQLKALHTSAFNVINGDVIIEGVVTADDQSGNFYKEINIQDATGGLGVKINVT
                     NIYQSYPVGKKIAINCKGLTLGTDGDTKLGGNTYQSNGKDLLGGLDAAQMASNIVING
                     TGTPTPKPVTINSLGEADFSNFVILKDVQFVSGDVGQPYADAAGKMTLNRIVEDCDGN
                     TITVRTSGYADFASSLAPGGKGTLVGIYENFGNTKQLFIRSAADASMSGSRCSTTPTP
                     GLEELDEDFESQQNEVDISLQGWTNIAVAGTRIWRGKVFQGNAYAQATAFKSSDATNT
                     TWLVTPGMDLSTPKTLSFESAKQVWVHDGLSVWISYDFNGGNAASATWTEISCTLAGK
                     NDPDNTFIQSGIIDLPVNTSKAYIGFKYTGDKTTNTTSYRIDNVKIKKK"
     gene            4532..5248
                     /locus_tag="PLE23_09120"
     CDS             4532..5248
                     /locus_tag="PLE23_09120"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMX83464.1"
                     /translation="MGSKDIFRFQQFEVKHDKVGLKVGTDGVLLGAWADVAGAQDILD
                     IGTGSGLIALMLAQRHPSANIHAIDIMIAASEQAIENFNVSPWAENLRCFHISAQDFA
                     EATAQRYDLIVSNPPYFSDGLLSGDKGRASARHAISLPRDELVSSVKTLLRPSGRFCV
                     ILPYLEGLRFKEIAQTAGLFCNKTVGVKPDIYSSINRWLMEFSVKASRYQYSEIFLKN
                     ETGGRSTVFNSLTSEFYLNV"
     gene            complement(5284..5547)
                     /locus_tag="PLE23_09125"
     CDS             complement(5284..5547)
                     /locus_tag="PLE23_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMX83465.1"
                     /translation="MNITFNELRNIKHKLPHGSVKKIADHLNIDEQTVRNYFGAHKVD
                     NGGIVDQHIQPGPEGGIVFLEDTTILNMALEILKEHEAEANEN"
BASE COUNT         1717 a         1131 c         1267 g         1534 t
ORIGIN      
        1 aagaatttcg tgcaaagcag aatttgccga tcaggcttaa agcaaatgag acagctcaac
       61 aaggccagaa tattgcagtc attgccctgc caagtggtaa tgaaggtgat gatgatgact
      121 acgaagtaag cagcttgctt gcagcttcga gggatgtatt caattccatt gcaggattca
      181 atttttcatc tgccggcttt gacctccgcg gttacgagag cgaataccag tctgtttata
      241 tcaataatgt accggtcaac gatccggagt ctggcaacag tatttactca acatggggcg
      301 gcctcaatga agtgatgcgt accgacaggt ttctgaatgg cctgagtgcc tttagtaacg
      361 gattcggcaa cattggaggt acagccatta ttgatgcttc agcagctacc caacgcaagc
      421 aaaagaaagt tagctacatg ttctctaatc gttcgttcca gcatcgtttg atggccaccg
      481 tcagcaccgg tacaatgaaa aacggctggg cattcagtgc atcaggttcg cataggtggg
      541 caaacgaggc atacgtgccg ggtacatttc ttgattcatg ggcttattat ctgggtgctg
      601 agaaaaaatt ccgcaacggc gaccagttta gtgttactgt gtttgggtca cccactcgca
      661 gaggtaaggc agctcctagc gttcaggaaa tgaacgacct ggcaggtacc aattattata
      721 acccaaactg gggctaccaa aacggagaaa aacgaaatgc cagagtagcc aatacacatt
      781 tgcctacggc catcgttggg tacaacaaaa aatttaatgc caaagctcag tggaataatt
      841 cactcttgtt tcagacaggt cgtgaaggca attctgccct tgactggttt gacgctccgg
      901 atccacgccc cgactattac cgccgattgc cgagttatgc cgaaaaccct gacgtagccg
      961 cacagattgc agcactactc aagtcagacg aaaaagcacg ccagattaat tggcaagaat
     1021 tatacaatat caatgatgcc tcttacggta cagttgagaa tgcaaatggt atatcaggca
     1081 ataacgtgtc aggcaaaatt tcaaaatacc ttatcagtga acgccgcaca gatcagacag
     1141 ttttgagcct gaacagcaca ttcagttatg aactcagttc agttgcaaaa ttgtcagccg
     1201 gtgtgttggc acgtagcttc aaaggtaaaa attttcagac tgcacttgac ctgttgggtg
     1261 gcgactttat tgtcgataat gacaaatatg cagaacgcga tttcccgaat gattcactgg
     1321 cattacaaaa tgacctggac gtactaaaca aaatcgtgaa ggaaggcgaa caatacggat
     1381 ataattacaa tacacatatc agaagcgctc agccttgggc acagcttgat attacgcttc
     1441 gcaaatttga tatttttgca gctgccaatg taggttttac aaatatctgg cgagaaggac
     1501 ttgtgcgcaa cggtcgtttt ccggataatt caaaaggcaa ttcggagaaa caaaaattta
     1561 ccacttacgg tatcaaggca ggtacaacat acaagcttaa tggtcgtaat tatctttatg
     1621 ccaatgcagg atatatgacc caggcaccgt tggttcgtga cattttccag tcgccacgta
     1681 ccagaaatac caccattcct aatctgacaa cagaaaagac actatcagct gaagcaggct
     1741 atatattgcg agctccggtg ctaaaggcaa gggtatcagc atattataca caatttcgcg
     1801 accttgcacg aactacatca ttttatcatg atgagctgcg tacatttgtc aactttaacc
     1861 tgaacggtat cggcaggacg cacattggta ccgagatagg actcgattac aagatttcgc
     1921 cttcattttc agtacaagct gcagctaatt taggagacta ttattatgac acccgggctt
     1981 atgcagatat ttctcaggac aataataata ccttggtagc tgatgaccgt cttgtgtatc
     2041 aaaaagattt ctttgtagcc aatacaccgc agacagcagc aggcctcaac cttcgttatg
     2101 cagggccacg cagttggttt gccactctga gtgctagtta ttttgataaa atgtatatag
     2161 actttaactc ggacagacgt acgaatatag ctgtcagtgg actcgatgat gcctcagaaa
     2221 catatctgaa gataatagaa caagaaaggc ttgaccctgc atacacaatt gatgcctcaa
     2281 tcggtaagaa ttggcgggtg aaaaaaacat ttatcacact taacatttcg gtcaataatc
     2341 tgttgaataa tacgtcattt aagactggcg gattcgaaca gctcagattt gactatgcag
     2401 ataaagacat taacaaattt cctccaaagt atttttacct ttacgggctt acgtatggag
     2461 taaatctagg tgtatcattt taattaacca ggaataaaac aaaattatga taaagtcaat
     2521 ttactacatt tttctgagcc tcatcatgac cgggctggtt gcttcttgtg tcaagcatga
     2581 gtttgacaaa ccgccagtga gtacagaaga ccccaacttg actgccaata ctactattgc
     2641 cgaactgaag gcattacatt cagcaggaaa atttgacgta ataaccgatg accggattat
     2701 agtaggtatc gttattgctg atgatgagtc gggcaattat tacaaaacca tcgtcataca
     2761 agatcaaacc ggaggtatag aattgaagat caacgccacc gatcagtatg tcaaatatcc
     2821 ggtaggccga agactattta taaaatgtaa gggactcaca ctcggtgatt acaatggagt
     2881 agtgcagctt ggaggtggag tttttgacaa caacggtagc ccttcacttg gtggtatcga
     2941 gcaggcactt acagatactt atattttccc gggccagtat aaacaatacg ttacacctaa
     3001 gaagctcaaa attaccgaac ttactcaggc agatgtgagt acgcttgtag agttagaaga
     3061 tgtcgagttt gcttcaggcg aatcagggaa aacctatgca gatgtagtag gcaagacaac
     3121 gcaaaacaga accgttgagg actgtaacaa gaacgtggta atcatgcgca caagtggata
     3181 cgccaatttt gcaggtgact tgctgcctac cggcaaagga acacttacag cagtatatag
     3241 catttttggt tctacaaagc aggtatatat aagagataca cgtgatgttg tattcacagg
     3301 aacacgttgt ggcggtggct ccggtaccgt aacaccgaat actaccatag cacaactaaa
     3361 ggcactccac acttcagcat ttaatgtcat caacggcgat gtcatcatag aaggtgtagt
     3421 aacagcggat gatcagtccg gcaattttta taaagagata aatattcagg atgcaacagg
     3481 tggtcttggt gtaaaaataa atgttacgaa tatttaccag tcgtatcctg ttggtaaaaa
     3541 aatagccatc aattgtaagg gattgacttt aggtacagat ggtgatacca agctgggagg
     3601 caatacttat caatcaaatg gaaaggactt gcttggtggt cttgatgcag ctcaaatggc
     3661 atccaacatt gtcataaacg gtacaggtac acctacgccc aaacctgtaa ctatcaatag
     3721 tctgggtgaa gccgacttta gtaattttgt gattctaaaa gatgtacaat tcgtgtcagg
     3781 tgacgtaggg cagccctatg ccgatgcagc cggcaaaatg acactcaaca gaatagttga
     3841 agactgtgac gggaatacaa tcacagtacg tacaagcgga tatgccgatt ttgcatcttc
     3901 gttggctcca ggtggcaaag ggacgctggt aggtatttat gaaaattttg gtaataccaa
     3961 acaattgttc atccgatctg cagctgatgc aagtatgagt ggttcaaggt gttccacaac
     4021 gcctactccg ggccttgaag agttggatga agactttgaa agccagcaaa atgaagtgga
     4081 tatcagcttg caaggttgga caaatattgc agtagcaggc actagaatct ggcgaggaaa
     4141 ggttttccag gggaatgcct atgctcaggc tactgccttc aaatcgtctg atgctactaa
     4201 tacaacctgg cttgtgacac ctggaatgga tctttcaaca cccaaaacac tttcattcga
     4261 atcagccaaa caggtgtggg ttcatgatgg tctgagtgtt tggatttctt atgatttcaa
     4321 cggtggcaat gcggcatcag ctacttggac agaaataagc tgtacattag caggaaagaa
     4381 tgaccctgat aatacattta ttcagtcagg tattatagac ctgccggtca atacttcaaa
     4441 agcatatatt ggctttaaat ataccggtga caagacaacc aataccactt catacagaat
     4501 cgataatgtg aagattaaaa agaagtaggg cttgggttca aaggatattt tcaggtttca
     4561 acagttcgaa gtaaaacacg ataaagtagg tctgaaggtt ggtacagatg gtgtgttact
     4621 cggtgcttgg gcagacgttg caggagcaca agacatcctt gatataggta caggtagtgg
     4681 cctgatagct ctcatgcttg ctcaacgaca cccgagtgca aatatccatg ctatcgacat
     4741 catgatagcc gcgagcgaac aggctataga aaattttaat gtttcacctt gggcagagaa
     4801 tctgagatgt tttcatattt ctgcccaaga ctttgcagaa gccactgcac agcgttacga
     4861 tctgattgtg tcaaatccgc catatttttc tgatggatta ttatccggtg ataagggtag
     4921 ggcatctgcc agacatgcca tatcattgcc acgggatgag cttgtcagca gtgtaaagac
     4981 gttactgagg ccaagtggtc ggttttgtgt tattttgcca tatcttgaag gattgcgttt
     5041 taaggaaata gcacagacgg caggcttgtt ttgtaacaag acagtaggag taaaacctga
     5101 tatctatagt agtatcaatc gttggttgat ggagttttca gtcaaagcaa gccggtatca
     5161 gtacagtgaa atattcctta agaatgaaac cggtggcagg tcaacagtat tcaacagctt
     5221 gacttccgaa ttctacctaa atgtataaaa aaaacggcgg atattgattc caccgttttt
     5281 ttattagttt tcgttagctt ctgcttcgtg ttctttcaga atttccagag ccatattaag
     5341 gatggttgta tcttcaagga atacgattcc gccttcaggg ccaggctgaa tgtgttggtc
     5401 aacaattcca ccattgtcaa ctttgtgtgc gccaaaataa tttcggacgg tttgttcatc
     5461 aatgtttaaa tggtctgcta tcttttttac gctaccgtgt ggtagtttgt gtttgatgtt
     5521 cctcaactca ttgaaagtaa tattcataaa cctttagttt tagaaagtta aataatagga
     5581 tgaatcaatg tctaaatata gatgttattg ttgtgaaata aaaatttttg ccctgatttt
     5641 ttttggatt
//
LOCUS       DAOQQY010000189         5640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6324_length_5640_cov_6.087735, whole genome shotgun sequence.
ACCESSION   DAOQQY010000189 DAOQQY010000000
VERSION     DAOQQY010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6324_length_5640_cov_6.087735"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            65..556
                     /locus_tag="PLE23_09130"
     CDS             65..556
                     /locus_tag="PLE23_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83466.1"
                     /translation="MHLKNYNEKKYQDCRKRMLAQTKKEGLPQIVCSDISSSAVHIAK
                     LNARAAGVYDFITFEVCDFRDTTIIPDQAGVVVLNPEYGERLGEVEELEGVYTEIGDF
                     FKKHCTGYTGYVFSGNLDLLKNIGLKPKRKIPFMSAKIECKLMEYELFSGSKKIKEQS
                     VQD"
     gene            complement(714..1754)
                     /gene="asnA"
                     /locus_tag="PLE23_09135"
     CDS             complement(714..1754)
                     /gene="asnA"
                     /locus_tag="PLE23_09135"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HMX83467.1"
                     /translation="MNSGLIIPTGYEPLMDVKQTEKAIKLVKDTFQQNLSSELKLRRI
                     TAPLFVMKGTGINDDLSGTERAVSFPVKHMGDAHAELVHSLAKWKRMMLADLEIPHSY
                     GIYTDMNAIRADEDLDNIHSLYVDQWDWERHISDSERNIAFLKQIVRKIFEAMKRTEY
                     LIHEAFPNIFPTLPDEITFIHTEELLKRYPTLSPSQREYAITKEHGAVFLIGIGHRLS
                     DGKKHDNRAPDYDDWITESEIGMNGLNGDLLVWNDILERSFELSSMGIRVNRDSLLKQ
                     LEIENCTKRTELYWHQRLLNNEMPLSIGGGIGQSRLCMYYLKKAHIGEVQASVWDTEM
                     IEKCKAANIFLL"
     gene            complement(1850..>2046)
                     /locus_tag="PLE23_09140"
     CDS             complement(1850..>2046)
                     /locus_tag="PLE23_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83468.1"
                     /translation="GKDSKSECKPGCTKDCCKGKDSKSECKPGCTKDCCKGKDSKSEC
                     KPGCTKDCCKGKDSKKKSKK"
     assembly_gap    2047..2146
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2147..3818)
                     /locus_tag="PLE23_09145"
     CDS             complement(<2147..3818)
                     /locus_tag="PLE23_09145"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMX83469.1"
                     /translation="MKNLIKFLLLILISNGMNAQSSEHPENPNKSLAYKWVDIVLEVT
                     ANDVDRDGALPTIQSRSTGIIFTAMYDAWAAYDEKAVGTMYGGTLRRPAVERTQKNKE
                     TAISYAIFNVLMDLHYRDSTYIRDQMKALGYQINNTSLDRTTPEGIGNLVAKAIIEYR
                     HHDGANQLGDEIGGTNGKRYSDYTFYKPVNTWDHVIDPDRWHPLPFVDGKGKTFFVDF
                     VTPQWYRVKPFGLESSAQFRAPEPPRYGSAQLKKEVDEVMDLNANLDHQRKTTIEFMR
                     DGPRSTGQAGHWLRFAQLVSIRDKNQLDTDVKLFFTVAVTAMDAFIACWDTKRFYDTA
                     RPWTQVRLYYKGQKIKGWGGPDKGTLLIPAEEWHPYSPANFVSPPFPAYISGHSTVSG
                     ACAKVLELFTGSDHFGVTESRICGALTETPGDPVSLPLPTFSATAELAGISRVLGGYH
                     IQTDNIEGLKTGRSVGEWDYKVTQGYINGTKEVNSEAFFNPESAPAIGKSYAPIIMAS
                     ASTCPPGCTKDCCKGKDSKSECKPGCTKDCCKGKDSKSECKPGCTKDCC"
     gene            4257..>5640
                     /locus_tag="PLE23_09150"
     CDS             4257..>5640
                     /locus_tag="PLE23_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83470.1"
                     /translation="MQHQDKCDESETLKLAAMIGLALLAFLLLLSGIFYKLRMSFLDG
                     PFMIFQIIEKDSPDIMVQRYGSIITQVWPWLAQKCSLPMNIIMLVYSLSFNMFYFLTG
                     AFLYKIRQYRWVIILAFYQIAFCTDAFFWPNNEIHQGIAWLAATAGLWFFIEHSGVKY
                     MGFRMLLAILLTISLFTHPLMLPVVIFLVILFVLEGVFKLNKREHQFILLFIFLVMVM
                     KFYLSTNNWYDGAKFSILASQSPNKWLQVFNSHAMHSFVQDLFSRHWVAVFIFAVTLW
                     VLIIQRKFPIALFMTIFTLIYLLLVAMAFPDFKRFYIESQWMPIAIFIITPLVFNFHV
                     FENKQRIVFFSLMVCIAWLMLMPGAVSMFQARYTRQMEILNRMETKNISKLILTDVPD
                     QIKEHIILPWALPVESIILSSASNTVGRRSFLFEQPERATKGTSIFLSAFENLDISTL
                     DRRYFWLNNTE"
BASE COUNT         1518 a         1075 c         1124 g         1823 t
ORIGIN      
        1 aactatatag atcaggtgca ttattgcaac acttattaag aacatagcca aaaattatgc
       61 tttcatgcac ttgaaaaatt acaatgaaaa gaaatatcag gattgtcgga agaggatgct
      121 tgcacaaact aaaaaggaag gattgccaca gattgtgtgt tctgacatca gctcaagtgc
      181 ggtacatatt gccaaactga acgcaagagc agccggagtg tatgatttta ttacattcga
      241 agtttgtgat tttcgggata ctaccatcat tcctgaccaa gccggagtgg ttgtcctcaa
      301 tccggaatac ggcgaacgct taggtgaagt cgaagaactc gaaggtgtat atacagaaat
      361 tggcgacttc tttaagaaac attgcacagg ctatacgggc tatgtgtttt ccggaaatct
      421 cgatttgttg aaaaacattg gactcaaacc caaaaggaaa attccgttca tgtcggcaaa
      481 aatagaatgt aaactcatgg aatatgaatt gttttcgggt agtaaaaaaa taaaagaaca
      541 gtcggtacaa gattgaatga atatatgaac ctgttcaacc atgcataatt aaactgtaag
      601 gtaattctta aacccggata acgcattcgg aatgcaaacg aaaatgattc gtaaaaaaaa
      661 tccatgaaaa agtgataaaa gcatagtgaa ctatgttttt tgggctattc gggttaaagt
      721 agaaagatat ttgcagcctt gcacttttca atcatttctg tgtcccaaac agatgcttgt
      781 acttcgccta tgtgcgcttt tttaagataa tacatacaca atcttgattg cccaatacca
      841 ccacctatag ataagggcat ttcgttattc aacaaacgct gatgccagta aagctctgtt
      901 cgtttggtgc aattttctat ttctagctgc ttaagcaagg agtctctgtt cactctgata
      961 cccattgaag aaagctcaaa cgaacgctcc aggatatcat tccataccaa caaatcaccg
     1021 ttcagaccgt tcattccaat ttcagattcg gtgatccagt cgtcataatc cggtgcccgg
     1081 ttatcgtgtt ttttaccgtc cgatagtcgg tgcccgatac caatgaggaa tacagcacca
     1141 tgctctttgg tgatggcata ctctcgttgc gacgggctga gtgtaggata tcgtttcaga
     1201 agttcttcgg tatggatgaa tgtgatttcg tcaggtaatg tgggaaaaat attggggaat
     1261 gcctcatgta tcagatattc ggtgcgcttc atggcttcga agattttcct gactatctgc
     1321 ttgaggaatg ctatatttcg ctcagaatct gagatatgcc gttcccagtc ccactggtca
     1381 acataaagtg aatgaatgtt gtcaaggtct tcatcggcac gtatagcatt catatctgta
     1441 tatattccat aactatgtgg aatctccagg tcagcaagca tcattctttt ccactttgcc
     1501 agcgaatgta ccaattctgc atgtgcatca cccatgtgtt tgacaggaaa acttactgcg
     1561 cgttcagtac cgctcaggtc gtcattgatg ccggtacctt tcattacaaa caacggtgct
     1621 gtgattcttc taagttttaa ttcagacgac aggttttgtt gaaatgtatc ttttaccagt
     1681 ttaatggcct tttcggtttg ctttacatcc atgagtggct cgtagcctgt tggtatgatc
     1741 aatccagagt tcatttagtt tatttattgg gttattccaa tatacggaat aaacaaagca
     1801 cagtgttcag agaatactgt gctttattta cattatttta gatacaaact tatttcttgc
     1861 tttttttctt cgaatctttt cctttgcagc aatctttggt acaacctggt ttgcattcag
     1921 atttcgaatc ttttcctttg cagcaatctt tggtacaacc tggtttgcat tcagatttcg
     1981 aatcttttcc tttgcaacaa tctttagtac aacctggttt gcattcagat ttcgaatctt
     2041 ttccttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgca gcaatcttta
     2161 gtacaacctg gtttgcattc agatttcgaa tcttttcctt tgcagcaatc tttagtacaa
     2221 cctggtttgc attcagattt cgaatctttt cctttacagc aatctttggt acaacctgga
     2281 gggcaagtag aggcagaagc cattataatg ggtgcatagg actttccaat cgcaggtgcg
     2341 gattcaggat tgaagaatgc ttcgctattc acttctttgg taccgttgat gtagccttgt
     2401 gtcactttat aatcccattc gcccacacta cgtcctgtct tcagaccttc gatgttatct
     2461 gtctgtatgt gatagcctcc caatacacgg cttatgcctg ccaattcggc tgttgctgaa
     2521 aatgtaggta gtggcaatga aaccggatcg ccgggtgttt cggttaaggc accgcaaata
     2581 cggctttctg ttactccaaa atggtcactt cctgtaaaaa gttctagcac cttggcacat
     2641 gcgccactca cggtactgtg accggaaatg taagcaggga aaggaggtga aacaaaattg
     2701 gcaggtgaat aaggatgcca ttcttctgca ggaatgagca gtgtaccttt gtcgggtcct
     2761 ccccaacctt taattttctg acctttgtag tagagtctta cctgtgtcca cggccttgct
     2821 gtgtcataaa aacgtttagt gtcccagcag gcaataaacg catccattgc agtgactgcc
     2881 accgtgaaga acaattttac atcagtgtcc aactggtttt tgtctcttat tgatacgagt
     2941 tgcgcaaacc ttagccaatg acccgcttgc ccggttgatc ttggaccgtc tcgcataaac
     3001 tctatggttg tctttcgctg gtgatccaga ttggcattca aatccatcac ttcatctact
     3061 tctttcttga gttgtgctga gccatagcga ggtggttcag gtgctctgaa ttgtgctgaa
     3121 ctttcaaggc cgaagggttt tacacggtac cattgcggag ttacaaaatc aacaaagaat
     3181 gtttttcctt taccatcaac aaatggcaat ggatgccaac ggtcaggatc gatcacatgg
     3241 tcccaagtgt taactggttt ataaaatgta tagtcgctat accgttttcc attagtgcca
     3301 ccgatttcat ctccgagctg attggcacca tcgtggtgtc tgtattcgat gatggctttt
     3361 gctaccaagt tgccgattcc ttcaggggta gttctgtcca gacttgtatt attgatctgg
     3421 taccccaatg ccttcatttg gtcgcgtatg tatgtagagt cgcggtaatg aaggtccatc
     3481 aagacattaa atatggcata gcttatggca gtttccttgt ttttctgcgt tctttctaca
     3541 gcaggtcttc tgagtgttcc accatacatg gtacctactg ctttttcgtc gtaagctgcc
     3601 catgcatcat acatggctgt aaatatgata ccagtagagc gtgattggat agtaggcaat
     3661 gctccatctc ggtcaacatc attggcagtg acttctaaca cgatatcgac ccatttatag
     3721 gctaaggatt tgttcggatt ttccgggtgt tcagaagatt gggcattcat tccgttgctg
     3781 atgagtatca gcaataggaa ttttattaag ttcttcatag taatagttta ttgtatcttt
     3841 atttttacaa tatgaaataa ttaaaaaaat tttaatatat ataataatta acatataaaa
     3901 accatgccaa ctaagccgtt tttagcattc gaaaaatgat aaatttgtgt ttgtttaagc
     3961 cgggggaagt tgttgtaatc ggttgatgta cagtgctatt attaagtcaa cgaagtcata
     4021 aagctgaatg tttcatttga atattaaaat ttcattagaa ccgttaaact aattttaata
     4081 tatcgatgct tcaagaaata tagcccatga atgaacaaaa tgattcttga cttttaaagg
     4141 atattctgcc tcggttgtgc atggccgatt ttttatcggt caattatttt aggatgatca
     4201 gttgatagtt ttgacatgtt tcttacattt gatttattaa tgataattga ttgacaatgc
     4261 agcatcaaga caaatgtgac gaatcagaaa ccctgaaact ggcagcaatg atagggttgg
     4321 cactgcttgc atttttgctg ttgctgtcag gtatttttta caaactgcgg atgagttttc
     4381 tggacggtcc tttcatgatt tttcagataa tagaaaaaga tagtcctgat ataatggtgc
     4441 agcggtatgg ttcgatcatt acccaagtat ggccttggtt agcacagaaa tgtagtttgc
     4501 cgatgaatat catcatgctt gtttattcct tgagtttcaa tatgttttat tttttgacag
     4561 gcgctttctt gtacaaaatc aggcaatacc gctgggtgat aatattggct ttttaccaga
     4621 tagctttttg cacagatgct tttttttggc ccaataatga aatacatcaa ggaatagcgt
     4681 ggttggcagc tacagccgga ctttggtttt ttattgagca tagcggtgtc aaatacatgg
     4741 gcttcagaat gttgctggct atactgctta ccatcagttt gttcacgcac ccgcttatgt
     4801 tgccggttgt cattttctta gtcatcctct ttgtattaga aggtgtattt aagttaaaca
     4861 aacgcgaaca tcaattcata cttctgttca tttttctcgt tatggtcatg aaattttatc
     4921 tcagcaccaa caattggtat gacggagcaa agtttagcat actcgccagt cagtcgccca
     4981 ataaatggtt gcaggtgttt aatagccatg ccatgcactc attcgttcag gacttatttt
     5041 caaggcattg ggttgctgta tttatttttg cagtgacctt atgggtgtta attattcaac
     5101 ggaaatttcc gatagctctg ttcatgacaa tttttacatt aatatatctc ttgcttgtag
     5161 caatggcttt tccggatttt aaaagatttt atattgagag ccaatggatg cccattgcaa
     5221 tttttatcat aactccgctt gtgtttaatt ttcatgtatt tgagaataag cagcggattg
     5281 tatttttttc attgatggta tgtatagctt ggttgatgtt gatgccgggt gctgtaagta
     5341 tgtttcaggc cagatatacc cgtcaaatgg aaattcttaa cagaatggaa acgaagaata
     5401 tttcaaaact gatacttacc gatgtacctg atcaaattaa agaacacatt atcctgccat
     5461 gggcactacc tgtagaatcc atcattttat catctgcatc aaacactgtt ggaagacgaa
     5521 gttttctgtt tgaacaaccg gaaagagcca caaaaggtac cagcatattt ctctctgcat
     5581 tcgaaaatct tgatatttca acacttgaca gacggtattt ctggctgaat aatactgagg
//
LOCUS       DAOQQY010000190         5612 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6376_length_5612_cov_6.301422, whole genome shotgun sequence.
ACCESSION   DAOQQY010000190 DAOQQY010000000
VERSION     DAOQQY010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6376_length_5612_cov_6.301422"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            698..1651
                     /locus_tag="PLE23_09155"
     CDS             698..1651
                     /locus_tag="PLE23_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMX83471.1"
                     /translation="MKPPYEISIEILKYISQISEKIGEINAKYLIKTNPTLRKQNQIK
                     TIHSTLSIEGNTLTEEQITAIIENKRVVGPEKDITEVINALGVYKNINKLKPHNEKDF
                     LKAHKLLLKNLITNSGKYRKQSVGIVKGSKVEHVAPPFENVPYLMNGLFEYLKDKSEL
                     TLIKSCVFHYEMEFIHPFLDGNGRMGRLWQTLILMQDYPIFEFLPFETLIANNQEAYY
                     KALSQSEKEGKSTKFIEYMLKIIDNSLSNLLNNSNKRLSGNERITIFLEQNSSEFTRK
                     DYMNFFKEISSATASRDLKNAVTNRIIDKFGDKKTTKYRKY"
     gene            2248..2796
                     /locus_tag="PLE23_09160"
     CDS             2248..2796
                     /locus_tag="PLE23_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83472.1"
                     /translation="MAKINPIIKDKIELLEKKELQKLVLKAASTSKQFHDYLLINYID
                     KEYGEQDLFDAAKSDLYMLYRKSYKGYSKELQLANMFAACNKRINEFGKVCRNKALEM
                     ELILDVLEIPFSNSSNSFTTCFTKYNYQVYLLLKKAITILKTKLHEDYHIQYAPRLNE
                     YLTVLHRTSSHLDYIYLLPKSI"
     gene            3042..3230
                     /locus_tag="PLE23_09165"
     CDS             3042..3230
                     /locus_tag="PLE23_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83473.1"
                     /translation="MNLKKALDDVPEDSILTIDTSECDFIHYDMLEIISEFDNKARDR
                     NITLDLKGIEKVNVTAIH"
     gene            complement(3610..4047)
                     /locus_tag="PLE23_09170"
     CDS             complement(3610..4047)
                     /locus_tag="PLE23_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83474.1"
                     /translation="MRSIIFALSLFASGILLAQESNTWKTLGKVTYKKKFDELLGFKV
                     DVPVFGKEVKALEGKIVELQGYIIPVEGYKSHKEFVFSAYPYSNCFFCGGAGPETVME
                     VKSIDPVKYSVNPIKIRGRLHLNDRDINRLMYFLTDARLVEWK"
     gene            complement(4099..5391)
                     /locus_tag="PLE23_09175"
     CDS             complement(4099..5391)
                     /locus_tag="PLE23_09175"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX83475.1"
                     /translation="MMYWFRLAWRNLADRPLSTLLTVLLLALGISISAFLLITERQLN
                     DNFSRNLAGVDLILGAKGSPLQTVLCNLYHIDSPTGNVEVNEVKAFLHPAHPLIRARV
                     PLLLGDSYKTHRLVGTTYEFLDFYQTGIATGRKWQKPFEAVAGSKAAKALSLKPGSSF
                     KSSHGLLPDVDEHDHLITITGILQPTGSIADQLILTSPQTVWYSHPHQSETHEDVEHI
                     HSDEANHHHPEHLKPDSTIDTSATQLISHPDRSVTSVLVRLKNHNFQALNLARNINEN
                     TAIQASNPAIELNRLYDTMGVGEQILRYLVLILLAVGGAAIFIALLQALKDRKYELAI
                     MRVMGGSPTQLAGLVLAESLLMCVLGLVIGLLIAHTGLWFFGTYAADKYKYSFDAWQW
                     TMQETRLCGIVLILGCLAAVFPAVSAYKTELSQTLTDS"
     gene            complement(5388..>5612)
                     /locus_tag="PLE23_09180"
     CDS             complement(5388..>5612)
                     /locus_tag="PLE23_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX83476.1"
                     /translation="STGERQRLSIARALVNSPAVLLADEPTSSLDDLRSSRVIELLKQ
                     LCVEQGSSLVIISHDTRLKSHFASQTIIHS"
BASE COUNT         1818 a          999 c         1239 g         1556 t
ORIGIN      
        1 ctgtcacgtc ttttccggtt tgtagcatca gcctcacggt ggtgggtatg gctacgcggg
       61 gcatgggtgt aggcagttgt aattgttttt cttgctgttg ttagcacggt gagtattccg
      121 gagtaaaggg ggccactgat tccggagcaa agggtgccac tttgggatgg ataaaaaatg
      181 agaaaaatcc ggagcaaagg gtgccacttt gggatggata aaaaatgaga aaaatccagg
      241 gcaaagggag ccacaaattt tcgatattta tcagtgatgt tttggaacta ttttggagaa
      301 aaaaaaccaa aatggcaaac cgactaatat caatgagtaa aggtatttta acccagaaaa
      361 tcctatagag catcaagtct aatgaatgtc attagagaat tagttgagtt tactggaaac
      421 agtcgctcag tttgcccgga attttcactt cgccaagtgt tgaattgttg cgtttgagaa
      481 atcgagaaat gattgtttaa tttttagtaa acacattcaa tcagcaatct gtcatatcat
      541 ctgaaaatat tcacgtacag ttagctgatt ttttggaaga cgcacaagtt gaaaaggacg
      601 aaggtgcaat atttttaaaa atttcaactt taattcagaa caaagatata cctttgtgat
      661 agtatcaaat gatagtatca catgaaagta tcacattatg aagccaccat acgaaatatc
      721 aatagaaatc ctaaaataca ttagtcaaat ttctgaaaaa atcggagaaa ttaacgctaa
      781 atatttgatt aagactaatc caaccttaag aaaacaaaat caaattaaaa cgattcattc
      841 tactttaagt attgagggaa acacattaac agaagaacaa ataactgcaa ttattgagaa
      901 taaacgggtt gtaggtccag aaaaagatat aactgaagtt ataaacgctt taggagttta
      961 taaaaacatc aacaaactaa aacctcacaa tgaaaaagac tttttaaagg ctcataaatt
     1021 gcttttaaaa aatctgatta ctaattcagg taaatacaga aaacaaagtg taggtattgt
     1081 aaaaggttct aaagtggaac atgttgcgcc accatttgaa aatgttcctt atttaatgaa
     1141 tggtttattt gaatacttaa aagacaaatc agaactcact ttgattaaaa gttgtgtttt
     1201 tcattatgaa atggaattta ttcatccttt tttagatgga aatggccgta tgggaagatt
     1261 atggcagact ttaatattga tgcaagatta cccaattttc gaatttttac ctttcgaaac
     1321 cttaattgca aataatcagg aagcatatta caaggctctt tctcaatccg aaaaagaggg
     1381 aaaatctacg aagttcattg aatatatgtt gaaaataatt gacaattcgc tatcaaattt
     1441 attaaataat tccaacaaac gtctttcagg aaacgaaaga ataaccatat ttctagaaca
     1501 aaactcatct gaatttacaa gaaaggatta tatgaacttc tttaaagaaa tatcatcagc
     1561 tacagccagt agagatctaa aaaatgcggt taccaacaga ataattgata aatttggaga
     1621 taaaaagact acaaaatatc gaaaatatta atattgagtt caatggccag tttggcgtca
     1681 ggtggggcgt atttcacgtt tgttattttt tagaatattt aaccttgtcg ctcaatatct
     1741 ttgctcttat acaaaagcac cacaaggcgg tttggaaaat acgcctgtac gtcttaaccg
     1801 atagtccagt tctttagcct tagtcattgt ctgagtcctc tgtatttacc cgtctcgttg
     1861 gggtacagct cgttgcttac ttcctctaaa gcctatcatc tccgataaag ttgtgttacc
     1921 atttcaaaga actaattcta tctttgtacc ctgtggtaac gcaggacaca ccgcagtttg
     1981 caacaggcag aagccctacg tttaagtatc atcaaaaaag tatttgaagg taagctcgtt
     2041 ccgtaagacc ccaatgatga acctgctgaa aagttgttgg agagaataag tgcagcaaaa
     2101 gaaaaagaag ccccatcggg gcgaaaggtt aatagaaaaa ataaaataat ttggcaataa
     2161 ataatgtaaa aaatatatta aatagagaat ttgggagcaa acattataat aaaaattggg
     2221 aaaactcaat gataaatcaa atattttatg gcaaaaataa accctattat taaagacaaa
     2281 attgaattgt tagaaaaaaa ggagttgcaa aagttggttt tgaaagcagc atctacaagt
     2341 aaacaatttc acgattattt attgattaat tacattgata aagaatatgg tgagcaggat
     2401 ttatttgacg cggcaaagag cgatttatac atgctttaca gaaagagtta caaaggatat
     2461 tctaaagaat tgcaattggc aaatatgttt gctgcatgca ataaacgaat taatgaattt
     2521 ggcaaagtat gcaggaataa agcccttgaa atggaattga ttttggatgt tctagaaatt
     2581 ccattttcca actcttccaa ctcgtttaca acatgcttta caaagtataa ctatcaggtt
     2641 tatcttttac taaaaaaggc aataacgatt ttaaaaacaa agcttcacga agattatcac
     2701 attcagtatg caccccgatt aaatgaatat ttaactgttc tgcaccgaac atcctcacat
     2761 ttggattata tatacttatt gccaaaatct atttaaataa tatgagcaac ggaaatacat
     2821 cagcaatact aaataaggtt tggagtttct gtaattcact tagaaatatt ggtgtgggtt
     2881 atggcgacta tttggagcaa cctacctaca ctagataaaa cacaaaaaca ctttttaaaa
     2941 cacgaaacct ttttcggaaa tgaaattgtg ccaaacacaa gaagaatgtg ttagatgaat
     3001 ttgtttcttc acactaatgg tgattagagt atctcaataa agtgaacctg aaaaaggcat
     3061 tagatgatgt tcctgaagac agtatattga caattgacac aagtgagtgt gatttcattc
     3121 actacgacat gctggaaatc atcagcgagt ttgacaacaa agcaagagac agaaatatta
     3181 cgttggacct taaaggaatt gagaaagtaa atgttacagc aatccattga cgacagaagc
     3241 aaggcagcgc ataatatcaa ccggattgaa aaagcccgac cacaaacgag agatacctta
     3301 ataaaccaaa gccccggatc ataaacgaac ggggcttttg caatccggtg tatagcgaca
     3361 tcgggcttgg acgttttctc ttaggtggat gtttgagtgc ttagatggtt tttagtctct
     3421 gtgttcaata ttcaaactta tcggaatgca tgagggatag aagccggcac gaagtaaggc
     3481 ggatagcccg acccgatagg ggcatgccca catcatgaag aaagacggca taatgttgct
     3541 acatagaaag cagtcgtgtt gacatgtgtg atgaggctga attagtaagg agcattaccg
     3601 aatgactggc tatttccatt cgaccagcct ggcatcggta aggaagtaca taaggcggtt
     3661 gatgtcccgg tcattgaggt ggagtctgcc gcggattttg attgggttta cgctgtattt
     3721 gaccggatcg atacttttaa cttccatgac ggtctcgggg cctgcaccgc cacagaagaa
     3781 acaattggag taaggatagg cagaaaaaac gaattcttta tgacttttgt aaccttctac
     3841 cggaatgatg tagccttgaa gttcgacaat cttgccttcg agcgctttca cttccttgcc
     3901 aaaaaccggc acatcgactt tgaagccgag tagctcgtcg aattttttct tgtatgttac
     3961 ttttcccagc gttttccaag tattcgattc ctgagcaagc aggatgcctg acgcaaaaag
     4021 actcagagca aatatgatag accgcatgat tttgaccaat ttgactacaa agaaagccga
     4081 aaaatgccaa taatgccctt aagaatcggt taaagtttgg gatagttcgg ttttgtaagc
     4141 agaaactgcc ggaaaaacag ccgctaagca tcctaaaatg aggactatgc cacaaagccg
     4201 ggtttcttgc attgtccatt gccaggcgtc aaaactgtac ttatacttgt cggctgcgta
     4261 agtaccgaaa aaccagagac cggtatgagc aatgagcagg ccgatgacaa gcccgagcac
     4321 acacatgagc aaagattcgg caaggacaag accggcaagc tgtgtagggc tgccacccat
     4381 tacccgcatg atggcaagtt cgtattttcg gtctttgagt gcctgtaaaa gtgctatgaa
     4441 aatagctgca ccacctactg ccaacaatat gagcaccaga taacgcagta tttgttcgcc
     4501 aacacccatg gtatcgtaaa gtctgttcag ttctatggcc ggattactgg cttgtatggc
     4561 agtgttttcg ttgatgttgc gtgcgaggtt gagtgcctga aagttatggt tcttaagcct
     4621 taccaaaact gaagtgaccg acctgtcggg gtggctgatg agttgggtgg cactggtatc
     4681 aatggtgcta tccggtttga ggtgttcggg atgatgatgg ttggcttcgt ctgaatgaat
     4741 atgctctaca tcttcgtgcg tctcactctg atgcggatgg ctgtaccaga ccgtctgagg
     4801 tgaggtaagg atgagttggt cggcgatgct gcctgtaggc tgcaggatgc cggtgatggt
     4861 tatgagatga tcgtgttcgt caacatcggg cagaaggccg tgcgagcttt tgaatgaaga
     4921 gccgggctta agagataggg cttttgcggc tttgctccct gctactgcct cgaaaggttt
     4981 ttgccatttg cgacctgtgg ctatacccgt ttggtagaaa tcaaggaatt cgtaggtggt
     5041 accgactagg cggtgtgtct tgtagctgtc gccaagtagc agaggtacgc gggcacgtat
     5101 aagcggatga gccggatgga gaaatgcctt gacctcattt acctcaacgt tgccggtagg
     5161 agaatcgatg tggtagagat tgcacagtac ggtctgtagc ggactacctt tggcaccgag
     5221 tataagatcg acgcctgcga ggttgcgaga gaaattatcg ttgagttgtc gttcggtaat
     5281 aagcaaaaat gcgctgatgg aaataccgag tgccaagagc aggactgtga gtagcgtaga
     5341 aagtggcctg tcggcgagat tgcgccaagc cagtctgaac caatacatca tgagtggata
     5401 attgtttggc tggcgaaatg agattttaaa cgtgtatcgt gactgataat gacgagggag
     5461 cttccttgtt cgacgcagag ttgcttgagc agctcaataa cccggctact gcgcaaatca
     5521 tcaagcgaag aagttggttc gtctgcgagt agcactgcgg gactgttgac caatgctcta
     5581 gcaatggaca ggcgctgacg ttcgccggtg ct
//
LOCUS       DAOQQY010000191         5578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6442_length_5578_cov_6.492124, whole genome shotgun sequence.
ACCESSION   DAOQQY010000191 DAOQQY010000000
VERSION     DAOQQY010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6442_length_5578_cov_6.492124"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLE23_09185"
     CDS             <1..327
                     /locus_tag="PLE23_09185"
                     /inference="COORDINATES: protein motif:HMM:NF017669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HMX83477.1"
                     /translation="RMDILTDAEISDICRYSLIAEKYNPTLNRTSAREILLGKLESAA
                     KPPAREQDTEPNTPQPDSRPTKPKDDWFGKVLKDPITRRIGNTVAREITRGILDVLGL
                     GGRRRR"
     gene            complement(342..875)
                     /locus_tag="PLE23_09190"
     CDS             complement(342..875)
                     /locus_tag="PLE23_09190"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HMX83478.1"
                     /translation="MITRIFIFLVAGLNLSSVAEAQSAVKWMSLEEAVEKSKTEKRKI
                     FIDIYTDWCGWCKVMEKETFQNPCIANYLNRNYYPVKFNAEQKKDIVFKGTVYSYVKT
                     GNGGYHSLAAAIMRGKLSYPTSVFLDENLNLIQPLDGYLNIREFEKVMVYFAQDRYRT
                     TPWPKYERTFESPVNCK"
     gene            complement(971..2017)
                     /gene="ribD"
                     /locus_tag="PLE23_09195"
     CDS             complement(971..2017)
                     /gene="ribD"
                     /locus_tag="PLE23_09195"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HMX83479.1"
                     /translation="MKKPYYFLKRCSDLSKYAIGNVSPNPMVGALIVHNQSIIGEGFH
                     KHFGSAHAEVNAIDSVNITDKSLIPESDLYISLEPCNFFGKTPPCTELILKHNIKNIF
                     VSNLDRTSGIDGQSLLYLQKNQVSVRLLQCEIPQYNPVRFRNVYAAHKRPYIVIKYAS
                     SLDGFIGRSNERIKLSNSLSDRWVHLLRSYCDGILIGKNTALSDQPQLTNRLVPGKSP
                     VKILIDTRLDVPPENGFYNSPGKKLVYNFIKDEQNGEIHYIKLTDNDDFLSQLLTDLY
                     HKNIGILLIEGGSYTISKFLEKELFDEIYEIRTRKVLKTGIPKPKNITDLKQEFQFRD
                     DIVLRKYVQRDRLC"
     gene            2084..2638
                     /locus_tag="PLE23_09200"
     CDS             2084..2638
                     /locus_tag="PLE23_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83480.1"
                     /translation="MLYKINLKNAEDEILLDDQVYEFLTTDPYLVKIDFINNLRKHSS
                     GCAVFQRTWKEGYRDYRNETIYLHKLIAEKFLAKTKTKEKKLVGALNGNKLDCRLENL
                     AYRSRAVASRMRVTKSREGYTGVYRENKRFRAVISMNKKTIHLGMYDTAEEAAMAYNK
                     KSMELFGEDGKQNKIKVNTKQGKD"
     gene            2842..3729
                     /gene="accD"
                     /locus_tag="PLE23_09205"
     CDS             2842..3729
                     /gene="accD"
                     /locus_tag="PLE23_09205"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HMX83481.1"
                     /translation="MGWFKRLKEGITTNTKNKKEVPDGLWNICPKCKTTLSVKQLEEN
                     FFKCTNCNYHFRIGSHDYYDLIFDNSKYTELFADIISVDILHFVDMKSYSDRLEESVK
                     KTNLLDSMTVAYGKVDKKPLVIAAMDFNFIGGSMGSVMGEKIARGVDYAIEQKCPFMI
                     ISKSGGARMMESAFSLMQMAKTTAKLTQLSTAGLPYFSLMTDPTTGGVTASFAMIGDV
                     HLAEPEALIGFAGPRVIRETIKKDLPEGFQTAEFLLEKGFIDLIVDRQSLKATLSDLL
                     NYFVQKETTTQHPVAEPST"
     gene            complement(3695..5296)
                     /gene="bshC"
                     /locus_tag="PLE23_09210"
     CDS             complement(3695..5296)
                     /gene="bshC"
                     /locus_tag="PLE23_09210"
                     /inference="COORDINATES: protein motif:HMM:NF021586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HMX83482.1"
                     /translation="MKVVKTSYDNITAISKFDLQFLKSDQVLKKYYAYTSDLDGIKQA
                     ILNKKSNYKFRKQLSEILKRQHANTEDSRVLEAIHTLKHDNCFCVTAAHQPCLFGGPA
                     YFIYKIASTIHLANTLTHHFPEYKFVPVFYLGSEDHDFEEINHLNLFNNRIVWQKENH
                     SGASGRLTTEGLTDVVQTILNLFENQTEKREILNILLNTSLHEQTYNLVIKHFIYQLF
                     GEYGLVVVDPDDVELKRLMIPAFKDELLNQTSSKLLQTTISQLESDKVKVQARGREIN
                     LFYLRDNLRERIVMENGLFRALNTEYCFTASEMESELQNHPERFSPNVILRPLFQESI
                     LPNVAFVGGGGEIAYWTELKPIFEYHTVQYPVLIRRNSVAIIDITTLNRLTKAGIEVS
                     DLQYPFEKIEQDYLRKNSEMLDFSEYEKSINTILEQMYDMVQIYDQNSTGRLMADKNM
                     MVNKITEWEKKLNKQIILKHETKLNQMKSAYEKIFPGQNLQERTDSCIWLIAHFGTEI
                     ISFLVHNLNPLDRQFMYLVQQPDVE"
     gene            5348..>5578
                     /locus_tag="PLE23_09215"
     CDS             5348..>5578
                     /locus_tag="PLE23_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMX83483.1"
                     /translation="MEYIALHEFLLNPSDALLLDVRSPAEYEAGHIPGAVNLPLFTND
                     ERAQVGTEYKQRSPEKALLLGLDIIGPKMSEII"
BASE COUNT         1752 a         1023 c         1042 g         1761 t
ORIGIN      
        1 aggatggata tacttacaga tgcagaaata tcagacattt gcagatattc acttattgct
       61 gaaaaataca acccgacact taaccggaca agtgccagag aaatacttct tggaaaactc
      121 gaatcagccg ccaaacctcc tgccagagaa caagatactg agcctaacac acctcagcct
      181 gacagcaggc ctacaaagcc taaagatgac tggtttggga aagtattgaa agatccaatt
      241 acaagaagaa ttggaaatac cgtagctcgc gagataacaa gaggaatact ggatgtcctc
      301 ggtctgggag gcagaaggcg cagataaaat atctaaccaa tttatttaca gttaaccggc
      361 gactcgaaag ttctttcgta cttaggccat ggtgtagtac ggtatctatc ctgagcgaag
      421 taaaccatta ctttttcaaa ctctctaata ttgagatagc catcaagtgg ttggataaga
      481 ttaagatttt catctaggaa tacagacgta gggtaactta gcttccccct cataatagct
      541 gctgccagcg aatggtagcc tccattaccg gtttttacat agctgtaaac agtacctttg
      601 aatacaatat ctttcttttg ttcagcattg aatttgacag ggtagtagtt gcggtttaga
      661 taattggcaa tacaaggatt ttggaaggtt tctttttcca ttaccttaca ccagccacac
      721 cagtcggtat agatatcaat aaatattttc cttttttcag tttttgattt ctcaacagct
      781 tcttcaagac tcatccattt cactgcagat tgtgcttctg caacactact caggtttaat
      841 ccggctacaa gaaatatgaa aatacgtgtt atcattaatt ataaatcttt gaatacgtta
      901 tacaaagtta ccatgctttc aacctaataa acaaagattt gtggtcgcaa ggcatgactt
      961 taaatttaat ttaacacaat cggtcacgtt gaacatattt tcttaacaca atatcatctc
     1021 taaactggaa ctcctgtttt aaatcagtta tgtttttcgg ttttggaatt cctgttttaa
     1081 gaacttttct ggtgcggatt tcatagattt cgtcaaacaa ttctttttcc aggaattttg
     1141 agatggtata actcccacct tcaatgagta agataccgat attcttgtgg tataaatctg
     1201 ttagcagttg ggataagaaa tcatcattat cagtcaattt tatataatgt atttcgccat
     1261 tttgttcatc tttgatgaaa ttatatacca gttttttccc gggtgaattg taaaagccat
     1321 tttccggagg aacatctagt cgtgtgtcaa ttaatatttt taccggagac tttccgggta
     1381 ccaaacgatt agttaattga ggctggtcgg aaagggctgt attcttgcct atcaaaatac
     1441 catcacaata tgaacgcagc aaatgcaccc atctgtcgga cagagaatta ctcagcttaa
     1501 ttctctcatt gcttctaccg ataaagccat cgaggctgga agcatattta ataacgatat
     1561 aaggtctctt gtgcgccgca tatacatttc gaaagcgaac agggttgtac tgtgggattt
     1621 cgcattgcaa cagccttaca cttacttgat ttttctgaag atataacagt gattgtccgt
     1681 ctatacctga tgttctatca agatttgaaa caaaaatatt tttaatattg tgttttaata
     1741 taagttctgt acaaggtgga gtctttccaa aaaaattgca tggctctaat gagatgtata
     1801 aatcagattc aggtatcaaa cttttgtctg taatatttac agaatctatg gcatttacct
     1861 cggcatgagc cgacccaaag tgtttgtgga agccttcgcc tataatgctt tggttatgaa
     1921 cgattaaagc tcctaccatt ggatttggtg aaacattgcc aatcgcatac tttgacaaat
     1981 ctgaacatct ttttaaaaaa tagtaaggtt ttttcatttc aattatgatg tcaaatatat
     2041 aattttgtaa aacactatta ataaccccac taaattaaaa aaagtgctgt acaaaattaa
     2101 tctaaaaaat gcagaagatg aaatacttct ggatgatcag gtttacgaat tccttactac
     2161 tgatccttat ttggtaaaaa tcgatttcat caacaatctt cgtaagcatt caagcggctg
     2221 tgcagtattt cagcgcactt ggaaagaagg ctaccgagat tatagaaacg aaaccattta
     2281 cctacacaaa ctaattgctg aaaaatttct tgccaagacc aaaactaaag aaaaaaagtt
     2341 ggtaggtgca ttgaatggta acaaacttga ttgcagactt gaaaatcttg cttacagaag
     2401 tagggcagta gccagcagga tgagagtcac taaaagtcga gaaggttaca ccggtgtata
     2461 tcgtgagaac aaaaggttcc gtgcagtcat ttcgatgaac aagaaaacca tccaccttgg
     2521 catgtatgac acagctgaag aggctgctat ggcttacaat aagaagtcaa tggagctttt
     2581 tggtgaagat ggtaagcaaa acaaaattaa agttaacacc aagcaaggaa aagactagca
     2641 gtccttggtt tttaaaaaag tttgaaatct acgtgttgtt tcgaaatgtg cgcatgttgg
     2701 gtttcactaa tcagtgcgcg caatagtcaa caaattgatt cttcgttgag attcttttaa
     2761 aaagataaaa catagccagt taattaattt ttggcatttt ttaatacctt tgacaattgt
     2821 taatattgca actgttagat tatgggatgg tttaaaagat taaaggaggg gattactact
     2881 aacaccaaga acaaaaaaga ggttcctgat ggtttatgga atatatgccc caagtgcaaa
     2941 acaacacttt cagtaaagca acttgaggaa aatttcttca aatgcaccaa ttgcaattat
     3001 cacttccgta taggttctca tgattattac gatttgatat tcgacaattc gaaatacaca
     3061 gagttatttg cagatatcat ttccgttgat atacttcatt ttgttgatat gaagagttat
     3121 tctgataggc tcgaagagtc tgtcaaaaaa actaatctgc ttgactccat gactgttgct
     3181 tacggtaaag tggacaaaaa gccgttggtc attgcagcaa tggattttaa ctttatcggt
     3241 ggttcgatgg gctcggtgat gggagaaaaa atagccagag gagtggatta tgctattgaa
     3301 caaaaatgcc cattcatgat aatttccaaa tcgggtggtg ccagaatgat ggaatcagca
     3361 ttttccctaa tgcaaatggc aaaaactact gccaagttaa cacaattgtc aactgccggg
     3421 ctaccatatt tttcattaat gacagacccg actacaggcg gagttacagc atcatttgca
     3481 atgataggtg atgtacacct ggctgagcca gaagcactca ttggttttgc agggcctcgt
     3541 gttattcgtg aaaccattaa aaaagatttg ccggaaggct tccagacagc cgaatttctc
     3601 cttgaaaaag ggttcattga cttaatcgtt gacagacaaa gtctaaaagc aacattgtca
     3661 gacctactca attattttgt acaaaaagaa acaactactc aacatccggt tgctgaacca
     3721 agtacataaa ctgtctgtca agtggattaa ggttatgaac aagaaatgaa ataatttcag
     3781 taccgaaatg cgcgattaac caaatacacg aatcggttct ttcttgtaaa ttttgaccgg
     3841 gaaaaatttt ctcgtaagca gatttcattt gattcagttt ggtttcatgt ttcaaaatta
     3901 tttgcttatt gagttttttc tcccattcgg ttattttatt aaccatcatg tttttatcag
     3961 ccattaatcg gcctgttgag ttctgatcgt agatttgcac catgtcatac atttgttcta
     4021 agatagtgtt gatgcttttt tcgtattctg aaaaatccag catctccgaa ttttttcgca
     4081 aataatcctg ctcgattttc tcaaacgggt attgtaggtc actgacttct atcccagcct
     4141 ttgtaagacg attgagcgta gttatatcaa taatggcaac actattccga cgaatcaata
     4201 caggatattg tacagtatga tattcaaata ttggcttaag ttctgtccaa tatgctattt
     4261 ctccgcctcc gccaacaaag gcaacgttag gtaagatact ttcctggaaa aggggccgaa
     4321 ggatgacatt ggggctaaac ctttcaggat gattctgcaa ttcgctctcc atttcactgg
     4381 cagtaaaaca atattcagtg ttcagtgccc gaaaaagccc attttccata acgattcgct
     4441 cacgcaaatt atctctaaga taaaacagat tgatttcacg tcctcttgcc tgaaccttta
     4501 ctttatcact ttccaactgt gatatagtag tctgcaataa ttttgatgaa gtttggttta
     4561 gtaattcatc tttaaatgca ggaatcatca aacgtttgag ctctacatca tctgggtcaa
     4621 cgactacaag tccatattct ccgaatagct ggtaaataaa atgttttatc accaagttgt
     4681 atgtctgctc atgaagtgat gtgttgagca gtatatttaa gatttcgcgt ttttcagttt
     4741 ggttttcgaa aaggttcaga atggtttgta ctacatcagt caagccttct gttgtaagtc
     4801 tgccacttgc tcctgaatgg ttttcctttt gccagacaat acgattattg aatagattaa
     4861 gatgattaat ttcttcaaaa tcatggtctt cacttccgag ataaaatacg ggtacaaatt
     4921 tgtactccgg gaaatgatgc gtaagcgtat tggcaagatg aatggtagaa gctatcttat
     4981 aaataaagta ggcgggacct ccaaacagac aaggctgatg tgctgctgtt acacaaaaac
     5041 agttgtcatg cttcagcgtg tgaatagctt ccaagacccg actatcttct gtatttgcat
     5101 gttgcctttt tagaatttca cttaattgtt tgcggaattt ataattcgat tttttgttta
     5161 atatagcttg tttaatacca tctaaatcag aagtatatgc ataatatttt ttaagtactt
     5221 ggtcagattt caaaaactgc agatcaaact ttgaaatggc agtaatatta tcatacgatg
     5281 ttttgacaac cttcattggt caattatttt ataactttat ggctcgaaat taacaaaaat
     5341 acaaagcttg gagtatattg cactacatga gttcctttta aatccctcag atgccctgct
     5401 acttgatgtg aggtcacctg ctgagtatga agccggccac atacctggtg ctgtaaatct
     5461 tcctttgttc accaatgacg aaagggcgca ggtcggaact gaatacaaac aaaggtcgcc
     5521 tgaaaaagca cttttattgg gtctggatat cattgggcca aaaatgtctg agattatc
//
LOCUS       DAOQQY010000192         5556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6485_length_5556_cov_5.799127, whole genome shotgun sequence.
ACCESSION   DAOQQY010000192 DAOQQY010000000
VERSION     DAOQQY010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6485_length_5556_cov_5.799127"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLE23_09220"
     CDS             <1..346
                     /locus_tag="PLE23_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMX83484.1"
                     /translation="NVFLWPSRSFVRKIFELQFTFMIEMLWPKERVMEVYLNVIEMGP
                     MTFGAEAAAKRYFRKSASKLSLEESALLIAILPSPLKWSATKPGPYVIKRQRWIIRQY
                     RMIGGTQYLKDL"
     gene            complement(373..1632)
                     /gene="metK"
                     /locus_tag="PLE23_09225"
     CDS             complement(373..1632)
                     /gene="metK"
                     /locus_tag="PLE23_09225"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMX83485.1"
                     /translation="MPYLFTSESVSEGHPDKVADQISDALIDNFLAYDPESKVACETL
                     VTTGQVILAGEVKSKAYLDVQEIAREVIRRIGYTKSEYMFESHSCGVLSAIHEQSPDI
                     NQGVERKKPEEQGAGDQGMMFGYASRETDNYMPLALELSHFLLLELAVIRREGKFMTY
                     LRPDAKSQVTIEYDDDNKPIRIDAIVVSTQHDDFDKEKTMLKKIEQDIKEILVPRVIK
                     KLPKRVQTLFDNKIKYHINPTGKFVIGGPHGDTGLTGRKIIVDTYGGKGAHGGGAFSG
                     KDPSKVDRSAAYAVRHIAKHMVAAGICDEALVQVAYAIGVAEPVGLFVNTYGTAKINM
                     TDGQIASVIKAIPEFNLRPYSIESRFKLRTPIYFESAAYGHMGRTPKEVVKVFNKGRD
                     NELKVKVKLFPWEELDQLPAMKKAFGL"
     gene            complement(1780..2388)
                     /locus_tag="PLE23_09230"
     CDS             complement(1780..2388)
                     /locus_tag="PLE23_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMX83486.1"
                     /translation="MKIFCVGRNYAAHAQELNNDLPDNPLIFMKPSTAILHKDRDFYI
                     PDFNNNIHYEAELVLKIAKNGKSIEPKFASKYISAITVGLDFTARDLQDQLKKNGHPW
                     EIAKAFDGSAVIGEWQDVRHYQPANTSFSLDINGVKVQQGNTTMMLFSFDKIISYISR
                     FFTLQTGDLIFTGTPAGVGAIKCGDVLEGFLENEKVLECKIK"
     gene            complement(2412..3416)
                     /gene="trpS"
                     /locus_tag="PLE23_09235"
     CDS             complement(2412..3416)
                     /gene="trpS"
                     /locus_tag="PLE23_09235"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMX83487.1"
                     /translation="MQRILSGIQPTGDIHLGNYFGAIRNWKDIQDDYICFFSVVDYHS
                     MTMPYKASSLRENTWKMVFQLLACEIKPEKLFIQSLVPEHTELCWILSCVCSFGELSR
                     MTQFKDKMDQLKTQDKDSLASAGLFMYPALQAADILIYRADKVPVGKDQEQHLELARN
                     IAERFNYQFGKEYFKSPEPLFTEIPKLLSPADPEKKMSKSLGEKHYINIFGDEAFIRK
                     QIKSAVTDTGEPVEGKMSPGVGNLFTLLKACDNMTAHDSLMSDFTTGQLKYSILKETV
                     ADTLIGFLSPFKQRYTEILADKPKYKKLIQESSAEIRKEAQQTLKDVRELTGLMNLKY
                     "
     gene            complement(3426..3809)
                     /locus_tag="PLE23_09240"
     CDS             complement(3426..3809)
                     /locus_tag="PLE23_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HMX83488.1"
                     /translation="MKKIVLTSNAPAPIGPYSQATIHNSVLYVSGQIAIDPATGELVN
                     SDIEAETHMVLKNLGAILTEAGIGFEHVLKCSVFVKDMNMFGRINAVYAQYFTGEQPP
                     ARELVEVANLPRFVNVEISAIAAIS"
     gene            complement(4120..>5556)
                     /locus_tag="PLE23_09245"
     CDS             complement(4120..>5556)
                     /locus_tag="PLE23_09245"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMX83489.1"
                     /translation="RNNFAKAIEYLNRAESELLSLPQLTIKDKYFLSLVFSGLGTVYD
                     KTSENKKATEVYGKVIALCEESGITTRLAWHYLNLGNCYLNDTQIDKAAMYFRKAITC
                     KDDISRQARAAASANLGFCSSELGQNEEALSYFEKAEVLYSDFIPPDTGNISVIMNGR
                     ALIYAKLGDTHKAIAYFTQATEYAKQDKNFKQLAQICKDISAFYKEQNDYKTAYEYLM
                     LYDDFNKKATEELNSRLLIENEVKYEAEKKEKEAELLRLQATELQLKALRAQMNPHFM
                     YNALNSIQHYITSYEISSASTYLAKFAKLMRQSLDYSEMQVISLEKEVEFLQDYLYIN
                     KKLRFQDKLEYEILVDAEIEDDVVGMPAMIVQPYVENAIEHGLRLKNDGKVMVEFLQH
                     DDDTIKCVISDNGIGRRQAFELRAKGAYGHKHKSRGTDITEKRLSLLKSKDGASGGVT
                     IIDLHDPVTGEAVGTRVEILIPIQDVMH"
BASE COUNT         1508 a         1139 c         1152 g         1757 t
ORIGIN      
        1 aaatgtgttc ctctggcctt ccaggtcttt tgttagaaaa attttcgaac ttcagtttac
       61 ctttatgata gagatgctgt ggccaaagga gcgtgtaatg gaagtatatc tgaatgtcat
      121 tgagatggga cctatgactt tcggtgctga agcggcagca aaaagatatt ttcgaaaatc
      181 ggcatctaaa ctctcactgg aagaatctgc cctgctcata gccatactac caagtccgct
      241 gaagtggagt gcaacaaaac caggcccata cgttataaaa agacagagat ggatcattcg
      301 gcaatacagg atgattggcg gaactcagta tctcaaggat ctatgaaata cctgctaaaa
      361 taatgcgttc aattacaaac caaacgcttt tttcatagcc ggcagctgat ctagttcttc
      421 ccaaggaaag agttttacct taactttcaa ctcattgtca cgtcctttat tgaagacttt
      481 cactacttcc ttaggtgtgc gacccatgtg tccgtaggct gcagattcaa aataaatagg
      541 tgtacgcagc ttaaaacggg attcgatgct gtaaggtctt aggttaaatt caggtatagc
      601 ttttatgact gaggctattt gaccatctgt catatttatt ttggctgtac cgtatgtatt
      661 cacgaacagt ccgacaggtt ctgccacacc aatggcataa gctacttgta caagtgcttc
      721 atcacaaata ccggcagcta ccatgtgttt ggctatatga cgaactgcat aggcagcact
      781 tctatctact ttgctagggt cttttcctga gaatgctccc ccaccgtgtg cgcctttgcc
      841 accataagta tcaacgataa ttttcctacc tgtaaggcct gtatctccgt gtggaccacc
      901 aataacgaat ttcccggtag gattgatgtg gtatttgatt ttattgtcaa acaatgtctg
      961 aacgcgttta ggcaactttt tgatgacgcg tggtacaaga atttccttta tgtcttgctc
     1021 tatcttttta agcatggttt tttccttgtc gaaatcatca tgctgagttg atacaacaat
     1081 agcgtctatc ctaatcggtt tgttatcgtc atcgtactcg atcgtaactt gcgacttggc
     1141 atcagggcgt aggtaggtca tgaatttgcc ttcacgtctg atgactgcta gctcgagtaa
     1201 gaggaaatgt gataactcaa gagcaagtgg catataatta tcagtctctc tggaagcgta
     1261 accaaacatc atgccctggt caccggctcc ctgttcttca ggttttttcc gctctacgcc
     1321 ctggttaata tcaggcgact gctcgtgtat ggcagacaag actccacaag agtgcgattc
     1381 gaacatatat tccgatttgg tatatccaat tctgcggatg acctcgcgtg ctatttcctg
     1441 tacatcaaga taagcttttg attttacttc acctgcaagt attacctgac cggtagtaac
     1501 caatgtttca catgctactt ttgattcagg gtcgtatgca aggaaattat caatcagcgc
     1561 atcagaaatc tggtcggcta ccttatccgg gtgcccttca gaaactgact cagaagtaaa
     1621 caggtacggc ataatatttg gttttttgac tttttttact caatctaatc acaaaagtaa
     1681 ggtattattc gctcttacaa aaaaatattt tgtttgagta caaagcgtca agcaggtaaa
     1741 ttcagatatt tcgataaata aaccacagcc ttttcaacat tatttgattt tacattcaag
     1801 taccttctca ttttcaagaa atccttcgag cacatcgcca cacttaatgg cacctacgcc
     1861 ggctggtgta cctgtaaaga taaggtcgcc ggtctgtaat gtaaaaaaac gtgagatata
     1921 gctaataatc ttatcaaaag aaaagagcat catcgtagta ttgccttgct gcactttgac
     1981 accgttgata tccagactaa agctagtatt tgccggttgg taatgccgga catcctgcca
     2041 ttcgccgatg actgccgatc cgtcaaatgc tttggctatc tcccatgggt ggccgttctt
     2101 tttgagttga tcctgcaaat cgcgtgcagt aaaatcaagt ccgacagtaa tggcacttat
     2161 gtattttgaa gcgaattttg gttcaattga tttaccattc ttggctattt tcaatacaag
     2221 ttcagcttcg taatggatat tattgttaaa gtcaggaatg taaaagtcgc ggtctttgtg
     2281 gagaatagct gtagaaggct tcataaagat aagcggattg tcaggcaggt cattgttgag
     2341 ttcctgagcg tgtgcagcat aatttcttcc gacgcaaaaa attttcataa atcggtatat
     2401 tttacagaat atcagtattt taggttcatt aagcctgtga gttcgcgtac atctttcaat
     2461 gtttgttgtg cttcttttct tatttctgcc gaagattcct gaatcaattt tttgtatttg
     2521 ggtttatcgg ccaatatttc ggtatagcgt tgtttgaatg gagacaagaa cccgatgagt
     2581 gtatcggcca cagtttcttt caggatgctg tacttgagtt gacctgttgt gaagtcactc
     2641 ataaggctgt cgtgtgcggt catattgtca caagctttga gcaatgtaaa cagattgccc
     2701 acacccgggc tcattttgcc ttcgaccggc tcacctgtgt ctgtcactgc agattttatc
     2761 tgtttacgaa tgaatgcttc gtcaccaaag atgttgatat aatgcttttc gccaaggctt
     2821 ttgctcattt tcttttccgg atcagccggt gaaagcagct tgggaatttc cgtaaaaagc
     2881 ggttcaggag acttaaaata ttctttcccg aactggtaat tgaagcgttc ggcaatgttt
     2941 ctggcgagtt cgaggtgttg ttcctgatct tttcctaccg gcaccttgtc tgccctgtaa
     3001 atcagtatat ctgctgcctg cagagccgga tacataaata atcctgccga ggcaaggctg
     3061 tctttatcct gcgtcttcaa ctgatccatt ttatctttaa actgtgtcat tcttgacaat
     3121 tcgccgaagc tacaaacaca acttagtatc caacagagtt cagtgtgttc tggtactagt
     3181 gactgtataa agagtttttc gggttttatt tcgcatgcca ggagctgaaa caccattttc
     3241 caggtatttt cacgaagtga tgaagccttg taaggcatgg tcatagaatg gtaatctaca
     3301 acactgaaga agcagatata atcatcctga atgtctttcc agttgcgaat agcaccaaaa
     3361 taattaccca gatggatgtc accagtaggc tgaatgccag agagaattct ttgcatagcg
     3421 agattttaag aaattgcggc aatggccgaa atttcaacat taacaaaacg aggaaggttg
     3481 gccacttcta ccagttcgcg tgccggtggt tgttcgcctg taaaatactg agcatacaca
     3541 gcatttatcc taccaaacat attcatatcc tttacaaata cgctacattt caatacatgt
     3601 tcgaatccta tacctgcttc tgtgagaatg gcaccgagat ttttcaacac catgtgtgtt
     3661 tctgcttcaa tatcggagtt gacgagttcg ccggtggcag ggtcaatggc tatctgcccc
     3721 gaaacgtaca ataccgaatt atgaatcgta gcctgcgaat aagggccgat gggagcaggg
     3781 gcatttgatg tcaacactat ttttttcatc ttttgtgtta ttaaaatttc ctaaaatatt
     3841 tactcctgac aaaattggtg ccttttatag aattagccaa aagaacggtg caatatttcg
     3901 tgaataatca ttaaaacttc agctaacggg aaaaatttta caaaaagaaa aaagcctgca
     3961 ctacatttgg gaaaggaggg caggcttcaa caaaacaaac atactatgag aaaaccgatg
     4021 taaagataag ctcctgtaca gcggctgaat caggcaaatg atggaatggt aagataaatg
     4081 cagaaaacgg tcaggtttat ttcagcaacg gtaaatgtat taatgcatca catcctgtat
     4141 tggtatcaat atctctacac gggtaccgac agcttcaccg gtaacaggat catgcagatc
     4201 aataattgta acaccaccgg atgctccatc ttttgatttg agcagtgaaa gtcttttctc
     4261 tgttatatcc gtacctcttg atttatgctt gtgaccgtat gcacctttag cccttagctc
     4321 gaatgcttgc ctgcgtccga ttccattatc agatatgaca cattttatgg tatcatcatc
     4381 atgctggaga aattcaacca tcacttttcc gtcatttttc aggcgcagac cgtgttcgat
     4441 agcattttca acataaggtt ggacaatcat ggctggcata ccgaccacat cgtcttctat
     4501 ttcggcatct actaatattt cgtattcgag tttgtcctga aacctcagtt ttttatttat
     4561 gtaaagataa tcctgaagga attcgacttc cttttccaga gaaatgacct gcatctcaga
     4621 ataatcgagt gactgtctca tgagtttggc gaattttgcc agatatgttg aagcagaaga
     4681 aatttcataa gacgtgatat aatgctgaat ggagttaagt gcattgtaca taaaatgcgg
     4741 gttcatctga gcgcgcaatg cctttaactg aagttcggta gcttgtaagc gaagtaattc
     4801 agcttctttc tccttctttt cagcttcgta ttttacctcg ttttctatga gtagtcggct
     4861 gttgagttct tcagtagctt ttttgttgaa atcatcatac agcatgaggt attcataggc
     4921 agttttgtag tcattctgct ctttgtaaaa tgctgaaata tctttacaga tttgggcgag
     4981 ttgcttaaaa tttttatctt gcttggcata ttcagtagcc tgtgtgaaat aggcaatggc
     5041 cttatgggta tcaccgagtt ttgcataaat aagtgcacgc ccattcatta ttacagaaat
     5101 gttgcctgtg tccggaggaa taaaatcact gtagagtact tcggcttttt cgaaatacga
     5161 gagtgcttct tcattctgtc caagttctga tgaacagaaa ccgaggttgg cagaagctgc
     5221 tgctctggct tgtcggctga tatcgtcctt gcaggtaatg gcttttcgaa agtacatggc
     5281 ggctttgtct atctgcgtat cgtttagata gcaattgccg aggttgagat aatgccatgc
     5341 cagacgagtg gttatgccac tttcttcaca caatgctatt actttgccat agacttcagt
     5401 agcttttttg ttttcggagg ttttatcata taccgtaccc aggccactaa acacaaggct
     5461 cagaaaatac ttgtccttga tagtgagttg tggtagcgac agaagttcag attctgcacg
     5521 gttaaggtat tctattgctt tggcaaaatt atttct
//
LOCUS       DAOQQY010000193         5500 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6582_length_5500_cov_6.100092, whole genome shotgun sequence.
ACCESSION   DAOQQY010000193 DAOQQY010000000
VERSION     DAOQQY010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6582_length_5500_cov_6.100092"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="PLE23_09250"
     CDS             complement(<1..961)
                     /locus_tag="PLE23_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HMX83490.1"
                     /translation="MTFDNFTVKAQEAIFEAQNLTSSLGQQTVDTPHLLGGILKAEES
                     VAEFLLSKCGVNIPVFKNQLEQLIRTYPKVEGGSKEYLSNDANKALSKAKSLLTEMGD
                     EYISVELLLLAIAAGNDKGSKLLKESGVSFDLLKKAISELRKGRKVTDQHSENTYNSL
                     NKYAINLTDRAEEGKLDPIVGRDDEIRRVLHILSRRKKNNPILIGDAGVGKTAIVEGI
                     AWRIVNKDVPENLRDKKIYTLDMAALVAGAKYKGEFEERLKAVINEVTSSEGKVILFI
                     DEIHTLIGAGGGNGAMDAANILKPALARGEIHTIGATTLDEYQK"
     gene            complement(1066..2784)
                     /locus_tag="PLE23_09255"
     CDS             complement(1066..2784)
                     /locus_tag="PLE23_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMX83491.1"
                     /translation="MNINAQPDLTTPWEKNRNITATYHECIDYYQLLDRQFDELKCEP
                     LYMTDSGFPLHQVVIDADRDFDPDASRKKGKTILFINNGIHPGEPEGIDATMMLARNL
                     LTDKDKHAWLKNTVVVIIPVYNIDGCLNRGPYSRVNQNGPEAYGFRGNDKNYDLNRDF
                     IKCDSQNARSFNRIFSYWNPDVMIDNHTSNGADYQHFITLITTQEDKLGPILGEFTRK
                     TVNPALYSGMNARNWHMVPYVNAWGPPDENGIDEFLETPRFSSGYAALHHCISFMPET
                     HMLKPFPDRVKSTYDLMEVMLDFLYRNGKDVQKARNEQISTFTNQQHVPITWKQDKSK
                     FEMIEFKGYEAVRKLSKVTGGERLFYDRTKPFTKMIPYYNKFETEKTVTKPFAYIIPQ
                     AYREVVDRLIMNKVHVEQLAENSRLMVENYRINSFETSPRAYEGHYLHSNTQVESFVK
                     EVQFRSGDYLVYTDQPAARYVIETLEPEATDSFFNWNFFDGILGQKEYFSDYIFEDLA
                     EQFLKENPEIARQLEQEKVKDEKLRNDPEAILDWVYRKSPWYEPTHQLYPVRRLMSPT
                     EISVKR"
     gene            2914..3750
                     /locus_tag="PLE23_09260"
     CDS             2914..3750
                     /locus_tag="PLE23_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMX83492.1"
                     /translation="MMNTVIVPAQISQEKEIENTINRFFKAKQKGDMALLDEMTVAEA
                     TFTTITNENRPNEVMDKTQLMNASIMIASNLPGTEEKLTNLNTRTDQYLATTTADYSY
                     FLNGKFTHCGQYTINLTKKSGKWRIMSSYETRHRDQCNNPTEEEINWLMDNWHLSAAK
                     ADAKTFFGSMAENGIYLGTDATERWTRDEMKVWAAKYFERGSAWDFKPIERKVYFSND
                     KRMAYFNETLNTWMGVCRGSGTLTKTTEGWKILQYHLAVTVPNDLIDGFIKLVDAPGR
                     KK"
     gene            3768..5177
                     /gene="rlmD"
                     /locus_tag="PLE23_09265"
     CDS             3768..5177
                     /gene="rlmD"
                     /locus_tag="PLE23_09265"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMX83493.1"
                     /translation="MKKRRREKNYTNILITGLADKGFGVGRDEAGEVVFVEKAVPGDV
                     VNVYSPRKKKNVRFGLVSEYVKLSPHRVRPFCEHFGTCGGCKYQHLDYKIQLQEKEKT
                     VQQALQRIGKVAPESFEPILAAEHTSHYRNKLEFAYSSLRWLTREEMDKGATSADNVL
                     GFHIAGVFDKILPIHECHLQVEPSNAIRNALRDIGEEQNLEFYDAKNHTGFLRNVVIR
                     ITTTGNIMVIVAFGKDDQKKVRNYLSEVKRQFPEITSLYYCINTKLNDYYADLDMVLY
                     EGEPVITEMLGNVKFNIGPKSFFQTNTHQAKQMFDIVKEYCGLTGRENVYDLYTGLGS
                     IALYIADSCRQITGIEEIAAAIDDAKINAALNNITNATFYAGDVKDILDDSFSQKHGK
                     PDVVITDPPRVGMHADVVSTLLKLEAPKIVYVSCNPATQARDIQLLSEKYRVTRVKPV
                     DMFPQTYHIESVALLELVK"
     gene            complement(5247..>5500)
                     /locus_tag="PLE23_09270"
     CDS             complement(5247..>5500)
                     /locus_tag="PLE23_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83494.1"
                     /translation="LQVYPNPSAGDIVTEITLPDYGLAQATELRVFDASGKEVYSWRY
                     PAYSYLHVIPAGRLSAGIYTASLMYKGSPVKFVKFSVVR"
BASE COUNT         1543 a         1219 c         1184 g         1554 t
ORIGIN      
        1 atttctgata ctcgtcaaga gttgttgcac cgatggtatg gatttcgcct cgtgcaagtg
       61 caggtttcaa tatgttggcg gcatccattg caccattgcc gccgcctgca cctatcagcg
      121 tatgtatctc gtcaatgaag agtatgacct ttccttctga gctggtcact tcattgatga
      181 ctgccttgag cctttcttcg aattcgccct tgtacttggc acccgctacc aatgcagcca
      241 tatcaagcgt ataaatcttt ttgtcgcgca ggttctcggg cacgtctttg tttacaattc
      301 gccaggcgat tccttctact atggcagttt tacctacacc agcatcacct atcagaatgg
      361 ggttgttctt tttccttctt gataaaatgt gcagtactcg tcttatttca tcatcgcgac
      421 caacgatggg atcgagtttg ccttcctcag cgcggtcggt caggttgatg gcgtatttgt
      481 tgagcgaatt gtaggtattc tctgagtgct ggtctgttac tttacgccct ttcctgagtt
      541 ctgatattgc ctttttgagt aagtcgaaac taacaccact ttcctttagc agtttacttc
      601 ctttatcgtt acctgctgca atggcaagta acagtaattc tactgaaata tactcgtcac
      661 ccatctctgt aagcagtgat tttgctttgc taagggcctt gttggcatca tttgacaaat
      721 attccttgct accaccttcc actttcggat aagtccgtat caactgttcg agctggtttt
      781 tgaaaaccgg aatgttgaca ccacacttgc taagtaaaaa ttcagcaact gactcctccg
      841 ccttcagaat gcctcccaac aaatgtggcg tatcaactgt ctgctggcca agagagctgg
      901 tcagattctg agcctcaaat atggcttcct gtgcctttac tgtaaaatta tcgaatgtca
      961 tatcgttgtt attttgtaga tactctctca aatcttaatc caattcaaaa aacctgcaat
     1021 tttgtcatat attaatgggt tatatatgtc atattgtcat aagatttatc tttttaccga
     1081 aatttctgtc ggactcatca gcctcctcac cggatagagc tggtgggtgg gttcgtacca
     1141 cgggcttttt cggtacaccc agtcgaggat tgcctccgga tcgttcctca atttttcatc
     1201 cttcaccttc tcttgctcca gttgtcgggc tatttccgga ttttccttca aaaactgctc
     1261 ggcaaggtct tcaaatatat agtcggagaa atattctttc tgtccaagga taccatcaaa
     1321 gaaattccag ttaaaaaatg aatctgtggc ttcgggttcg agtgtttcaa taacatacct
     1381 tgccgccggc tgatcggtat ataccaaata atcgcccgac ctgaactgta cttcctttac
     1441 aaagctttct acttgggtat tactatgcag ataatgccct tcgtatgcac gtggtgaggt
     1501 ttcgaagcta ttaattctgt aattttccac cattagacga ctgttttcag ccagttgttc
     1561 tacatgtact ttgttcatga ttaatcggtc cacaacttct ctgtaagcct gcggaatgat
     1621 gtatgcaaat ggtttggtca ccgttttttc ggtttcgaat ttgttgtaat aaggaatcat
     1681 cttggtaaaa ggcttcgtgc gatcatagaa gagacgttcg ccaccggtca ctttgctaag
     1741 tttgcgtact gcttcataac ctttaaattc tatcatttca aacttcgatt tgtcttgctt
     1801 ccaggtgatg ggtacatgtt gttggtttgt aaaagtggaa atttgctcgt ttcttgcttt
     1861 ttgaacatct tttccgtttc tgtacaggaa gtcgagcatt acttccataa ggtcgtaggt
     1921 gctttttacc ctgtcgggaa atggcttgag catgtgtgtt tcgggcataa aggaaataca
     1981 gtggtgcaat gcagcgtagc ccgaagagaa tcgtggcgtt tcaagaaact catcgatgcc
     2041 gttttcatca ggtggccccc aggcatttac ataagggacc atgtgccagt ttcttgcatt
     2101 cattcctgaa tacagtgccg ggttaacggt ttttcgggta aattcaccaa gtatggggcc
     2161 taatttgtct tcctgcgtgg taatgagtgt tataaagtgc tggtaatctg caccattaga
     2221 agtatgattg tcaatcatca catcggggtt ccagtagctg aatatccggt taaaggatcg
     2281 tgcattttgc gaatcgcatt taatgaaatc ccggttaaga tcgtagttct tgtcatttcc
     2341 tctaaaacca taagcttcgg gtccgttttg gtttactctc gaatatggcc cacggttaag
     2401 gcagccgtca atgttgtaca ccggaataat taccactacc gtattcttca accatgcgtg
     2461 tttatcttta tcggtgagca gattgcgtgc cagcatcata gtagcatcta tgccttccgg
     2521 ttcgccggga tgaatgccat tgttgatgaa tagtatggtt ttgccttttt ttcgacttgc
     2581 atccggatca aagtcgcggt ccgcatctat caccacctga tgaagcggaa atccggaatc
     2641 tgtcatataa agtggctcac atttcagctc atcaaactgc ctgtcgagta gctgataata
     2701 gtcgatacat tcgtggtatg tggctgtaat attgcgattt ttctcccaag gagtagtgag
     2761 gtcgggttga gcattaatat tcattgtgat aatattgatt gaacaaataa gaaaaaagaa
     2821 cttcatttga gtaattttga gctttaaatg tacaacctta tgtcgtttta taaaaacatt
     2881 tttcgaatac acacagcctt tctgttatac attatgatga atacagttat tgttccggct
     2941 cagatttcgc aagaaaaaga aatagaaaat accatcaatc gtttctttaa ggcaaaacaa
     3001 aagggcgata tggccttgct tgacgagatg acagtagcag aagccacatt cacgaccatc
     3061 accaacgaaa accgccccaa tgaggtaatg gacaaaactc agttaatgaa cgcttccata
     3121 atgattgctt ccaatctacc cggcaccgaa gaaaaactta ctaacctcaa cacccggacc
     3181 gatcagtacc tggcaactac cactgcagat tatagctatt ttttgaatgg aaaattcacg
     3241 cattgcggac agtacaccat taatctgact aagaagtcgg gcaagtggcg catcatgtca
     3301 agctatgaaa caaggcacag agaccagtgt aacaacccaa ccgaagaaga aatcaattgg
     3361 ctgatggaca actggcattt atcagctgcc aaggctgatg ctaagacatt ttttggttcg
     3421 atggccgaaa acggtattta cctcggtaca gatgctacag aacggtggac tagagacgaa
     3481 atgaaagtat gggccgccaa atatttcgag cgaggatcgg cctgggactt caaacccatt
     3541 gagcgtaagg tttatttttc aaatgataag cgaatggcgt acttcaatga aacactaaac
     3601 acatggatgg gtgtatgtcg tggttcgggc acacttacaa aaacgactga aggctggaaa
     3661 atactacaat atcatcttgc tgtcaccgta cccaatgatc tcatagatgg tttcattaaa
     3721 ctggtggatg caccaggtag aaaaaaataa acgattaaca ttcacagatg aagaaaagaa
     3781 gaagagaaaa gaattatacc aacattctca tcacaggact ggcagacaaa ggattcggcg
     3841 ttggacgaga tgaagcagga gaagtggtat ttgtagagaa agcagtgccg ggtgatgttg
     3901 taaatgtcta ttcgccaaga aagaaaaaga acgtcagatt cgggctcgta tctgaatacg
     3961 tgaagttgtc cccgcatcgg gttcggcctt tttgtgaaca tttcggcact tgtggaggat
     4021 gcaagtatca gcatctcgac tacaaaatac agcttcagga aaaagaaaaa accgtacaac
     4081 aggcattgca acgcatagga aaagttgccc ctgaaagctt cgaacctata cttgctgccg
     4141 agcatacaag tcattaccgc aacaaactcg aatttgccta ttcgtcacta agatggctta
     4201 cgagagaaga gatggataag ggtgcaacat cagcggacaa tgtgcttggt tttcacatag
     4261 caggtgtttt tgataaaatt ttacccattc atgaatgcca tctacaggtc gaaccatcca
     4321 acgccattcg gaatgcactg agagacatcg gcgaggagca gaatctggaa ttttacgatg
     4381 caaaaaacca taccgggttt ctgcgcaatg ttgtcatacg cattaccacc accggcaaca
     4441 tcatggttat cgtagctttt ggcaaagacg accaaaagaa agtcaggaac tatctgagcg
     4501 aagtaaagag gcaattccct gaaatcacca gcctgtatta ctgtatcaac accaaactca
     4561 acgattatta tgccgacctc gacatggtat tgtatgaagg tgaaccggtg ataacagaga
     4621 tgctgggcaa tgtgaaattc aacattggcc caaagagttt cttccaaacc aacacccatc
     4681 aggcaaagca gatgttcgac attgtgaagg aatattgcgg cctcaccgga cgagaaaacg
     4741 tgtacgatct gtacaccggc ctgggaagta ttgccctgta catagcagat agctgcaggc
     4801 agataaccgg aatcgaagaa atagctgccg ccatagatga tgcaaaaatc aacgcagccc
     4861 tcaacaacat aacgaatgcc acattctacg ccggcgatgt caaagatatt ctggacgact
     4921 cattcagtca aaaacacgga aagccggatg ttgtgatcac tgacccgcct agagtgggca
     4981 tgcatgccga tgttgtaagt accctgctca agctcgaagc accaaaaatt gtttatgtca
     5041 gttgcaatcc ggcaacacaa gccagagaca tccaattgct ctcagaaaaa tatagagtga
     5101 cacgggtaaa accggtcgat atgtttcctc agacttacca catagagagc gtagccttac
     5161 tggagctggt gaagtaaggc attcttacat gcacaacaaa agcgtaggca gaataattac
     5221 tgaaagtggt tcaaaataga ttttccctac cgcaccacac taaacttaac aaactttact
     5281 gggctgccct tgtacatcag cgaagcagta tagatacctg cactcagacg acctgcaggt
     5341 atcacatgca gatagctgta cgccggatac cgccagctat atacctcttt gccggatgca
     5401 tcaaacaccc tcagctcggt agcttgtgca agaccgtagt caggcagtgt aatctctgtg
     5461 actatatccc ctgctgaagg gttgggatat acctgcagac
//
LOCUS       DAOQQY010000194         5443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6688_length_5443_cov_5.395880, whole genome shotgun sequence.
ACCESSION   DAOQQY010000194 DAOQQY010000000
VERSION     DAOQQY010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5443
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6688_length_5443_cov_5.395880"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1162
                     /locus_tag="PLE23_09275"
     CDS             <1..1162
                     /locus_tag="PLE23_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83495.1"
                     /translation="FYYGGMQDNGTSGGNAAGINSWERLFGGDGFQPRFNPVDPNNYY
                     FETQNAGIYGTEDGQSFYDLINEDNGVPDVERRHWDAQYILSHFDPDVLYYGGEKMYR
                     SHDRGHTWTAISDNLTGDLKYLKGVHIISSLDESPLDSNILYAGTGNGYLWCTKDGGV
                     SWDTVQQNGLPERYLTSVHASPTLKNTVYVTFTGYKFNELIPHVFRSDDNGQTWTDIS
                     GDLPGIGINDILIIENEKDKVLYVATDGGVYGTVNGGLNWERLGTNMPVVPVLDIDVD
                     PVNKKLIAGTYARSIMTYPIGSITAVSENISEAEILTVFPNPIRSELFWNLTNGAGQY
                     DAVIRDTKGMIVRKMEGQAKQGNMNIDVLVPGIYVFSLESSTRKYIKKIVKH"
     gene            1184..2458
                     /gene="nhaD"
                     /locus_tag="PLE23_09280"
     CDS             1184..2458
                     /gene="nhaD"
                     /locus_tag="PLE23_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HMX83496.1"
                     /translation="MELILVATFLIGYALIAFEHSIKIDKAVPAILTGVICWVIYIFS
                     SNDTHLVNEQLLEHLGEVSGILFFLMGAMTIVELIDLHDGFRVITDRIKTKDKRRLLI
                     LFSVIAFFLSAILDNLTTAIVMGSMLGKILDDRNDRLYFASIIIIAANAGGAWSPIGD
                     VTTTMLWLGGQITTFGIIHKVFIPSVVSLLVPLLLMLRMVKGKVKPVESNGESSISVQ
                     DRNTIFYSGIGLLLFVPVFKTVTHLPPFMGMMFSVGIVWIISEYLHSKKEVEQKESIS
                     IFRALERMDMPSILFFFGILLAVSALQSFGTLNHLATSMSEHIPDERILVTIIGLMSS
                     IFDNVPLVAAVQGMYTLSQFPTDHFFWEYLAYCAGTGGSILVIGSAAGVAMMGIEKIS
                     FFWYLKKISMMALLGFLAGAGVSVLLSFYFHM"
     gene            2600..3412
                     /locus_tag="PLE23_09285"
     CDS             2600..3412
                     /locus_tag="PLE23_09285"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HMX83497.1"
                     /translation="MTLVLFLAGLFVVFVLNTDDLIKNFKENLELIVEIKTDVPRDEA
                     RNLLKKLEQGECVKLGSASFITREEGLSFLKNQLGHDVNVPIIENPLFDVIKFNIKAE
                     CIREDKMKALIAEIKANQQVNEVYFPIDLVSGISENIRRGSQLFLILSLIFGIIAVII
                     IHNTVKLALYANRFSIKTMEMVGASSATIKRPFLSTAVGIGLWSGLYASILLIGLLMF
                     LAQKFPDFIAFNNTYRLLWIVGGLILAGIIISFIITWFVINRYIGRKIESLY"
     gene            3424..3735
                     /locus_tag="PLE23_09290"
     CDS             3424..3735
                     /locus_tag="PLE23_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HMX83498.1"
                     /translation="MSKSNPKKVVISNTPRAKVETQGSRKSSSFFGSNEELIFNKENF
                     KWTGISLLLIFIGMLLMTGGSMPNPDTWDPNIIYSARRTVLAPIFILGGLVLQIYAIF
                     K"
     gene            3763..4569
                     /locus_tag="PLE23_09295"
     CDS             3763..4569
                     /locus_tag="PLE23_09295"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMX83499.1"
                     /translation="MTFIQAIIIAIIEGLTEFLPVSSTGHMVIVSSIMGIAADNFVKL
                     YEIVIQLAAILAVVVYYWKKFFDFTKIRFYLLLILATIPSLVVGYFLKSHVEKMLESP
                     LVIACILLLGGVVLLFIDKFFTQNTIQTDTKMTTGNAVFIGLFQTLSILLPGLSRSAA
                     TIIGGMQQGLTRQFAAEFSFFLAVPTMCAATVKSLYDVYKNTPEVLNTDNLDILLVGN
                     VVSFVVAFMAIKYFIEYLTKHGFRIFGIYRIILGALILILHFAGVKLFVH"
     gene            4569..5309
                     /gene="truB"
                     /locus_tag="PLE23_09300"
     CDS             4569..5309
                     /gene="truB"
                     /locus_tag="PLE23_09300"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HMX83500.1"
                     /translation="MAHSTTPILEEFLESQVLLIDKPLDWTSFDVVNRLRIALCRKLK
                     VKKLKVGHAGTLDPKATGLLIICTGKATKRITEFQDQAKTYTGQIFLGATRPSYDLET
                     EVDAQYPTDHISDEMIQYTTQSFVGIQQQMPPVYSAIKQDGKPVYKKAHKGEGDTVVM
                     QPREVIIYDFNTSSDALPLVSFEVKCSKGTYIRSLAFDFGKKIGTGAYLASLRRTASG
                     EFTVDDAWQLDDLIRYIDRYEPSVVVRT"
BASE COUNT         1572 a         1005 c         1266 g         1600 t
ORIGIN      
        1 tttttattat ggcggtatgc aggacaatgg aacttcggga ggtaatgctg ccgggattaa
       61 tagctgggag aggctgttcg gtggtgacgg gtttcagccg aggttcaatc cggttgatcc
      121 gaacaattat tacttcgaga cacagaatgc cggcatctac ggaacagaag acggacagag
      181 cttctatgac ctgataaatg aagacaatgg tgtgccggat gttgaacgcc gccattggga
      241 tgcacagtat attttaagtc atttcgatcc ggatgtatta tattatggtg gtgaaaaaat
      301 gtacaggagc catgacagag gacatacctg gacagccata tcagacaacc tgaccggtga
      361 tctgaaatat ctgaaaggtg tacacataat ttcgtcattg gatgagtctc ctttggattc
      421 gaatattctg tacgcaggaa caggtaacgg atacctgtgg tgtaccaagg atggcggtgt
      481 tagctgggat actgtacagc agaacggtct tccggagcgg tatctgactt cggtgcatgc
      541 ttcaccgaca ttaaagaata cggtatatgt cacctttaca ggatacaagt ttaatgaact
      601 cataccgcat gtgtttcgct ctgatgacaa tgggcagacc tggactgata taagcggcga
      661 cttgcccggt ataggcatca acgacatatt gattattgaa aatgaaaaag acaaggtact
      721 gtatgtagct acggacggag gagtatatgg tacagttaac ggaggattga actgggagcg
      781 actcggtacc aatatgcctg tagtacccgt acttgacatt gatgtggatc cggtaaacaa
      841 gaaactcata gcaggaacct atgccagatc aatcatgact tacccaatag gcagcatcac
      901 cgctgtaagt gaaaatatat cggaagcaga aattcttacc gttttcccga acccaatcag
      961 atcagaattg ttttggaatc tgaccaatgg tgccggccaa tacgacgctg tgatacgaga
     1021 tacaaaaggg atgatagtga gaaaaatgga gggccaggct aaacagggta atatgaatat
     1081 tgatgtattg gtgcccggta tttatgtttt ttcgttagaa tcaagcacac gaaagtatat
     1141 aaaaaagata gtaaagcatt aaacaacaat aaggaggcat gatatggagt taattctggt
     1201 agcaacattt ctcattggct atgcacttat agcattcgaa cacagtataa agattgataa
     1261 ggcagttccg gccatcctta ccggtgtaat ctgctgggtg atatacatct tttcgagcaa
     1321 tgatacccat cttgtaaacg aacagcttct tgagcatctg ggcgaagtat cgggtatatt
     1381 gttttttctg atgggagcca tgaccatagt agagttgata gaccttcatg acggattcag
     1441 ggtcatcaca gacagaataa agaccaagga taaaaggagg ttgcttatat tattttcggt
     1501 gattgcattt ttcctgtcag ctatacttga caaccttacc acggcaatcg taatgggttc
     1561 gatgcttggg aagatacttg atgaccgaaa tgacagactt tattttgcaa gtatcattat
     1621 cattgcagcc aatgcgggtg gtgcatggag cccgataggt gatgttacta ccaccatgct
     1681 atggttgggc gggcagatta ctaccttcgg aatcatacac aaagtattta taccgagtgt
     1741 ggtatcgttg ttagtgcccc ttctgctgat gcttaggatg gtaaagggaa aggtaaaacc
     1801 agtagaaagt aatggtgagt cgagcatatc agtacaggac agaaatacta ttttttattc
     1861 aggcatagga cttttgctgt ttgttccggt atttaagacg gtcacacatc tgccaccgtt
     1921 tatgggtatg atgttcagtg taggtatagt atggatcatc agtgagtatc ttcattcgaa
     1981 aaaagaagta gagcaaaagg aaagcatatc catcttccgt gcacttgagc gaatggacat
     2041 gccgagcata ctttttttct ttggtatatt actggcagta tcggcacttc agtcttttgg
     2101 gacactgaac catctggcaa ccagtatgag tgagcatatt cctgacgagc gcatattggt
     2161 aactatcatt gggcttatgt cgtctatctt tgataacgtg cctctggtag ctgccgtaca
     2221 aggtatgtac actttgagtc agtttccgac cgatcatttc ttttgggagt atctggctta
     2281 ctgcgccggt acgggtggga gcattttagt cataggatca gcagccggag tggcgatgat
     2341 gggaattgaa aaaatttcat tcttctggta tctgaagaaa ataagtatga tggcattgct
     2401 cggattcttg gccggggcag gtgtttcagt tttgttaagt ttttattttc acatgtaaag
     2461 gctggtgcaa tactgaaact accgataaaa tacatgagaa ttcatacctt atgcttatgt
     2521 ttgcagattg attatggcag gcaaacgaca atatacagaa accagtcaca aaccgggacg
     2581 cttatattct acgataagca tgacccttgt gctcttttta gcaggtctgt ttgtggtgtt
     2641 tgtgctcaat acggatgatc tgatcaaaaa cttcaaggaa aatcttgagc tgatagttga
     2701 aatcaaaacc gatgtaccac gtgatgaagc ccgaaacctt ctaaaaaagc tggagcaggg
     2761 cgaatgcgta aagctcggta gtgcatcttt tattacacgc gaagaaggct tgtcgttttt
     2821 gaagaatcaa cttggtcatg atgtgaatgt gccgataatc gagaatccct tgtttgatgt
     2881 aataaaattc aatattaaag ccgaatgcat cagggaagat aaaatgaaag ccctcatagc
     2941 tgaaattaag gcaaatcagc aagtaaatga agtatatttt ccaattgatc tggtttcggg
     3001 aatatcagag aacatccgaa gaggttcgca acttttcctg attctgagtc ttatatttgg
     3061 tattatagcg gttattatca ttcacaacac agtaaagttg gctttgtatg ctaaccgttt
     3121 tagtatcaaa acgatggaga tggtgggtgc gagtagtgct actatcaaac gcccattctt
     3181 gtctactgcg gtaggcatcg gtctgtggtc agggttgtac gcatcaatct tgttaattgg
     3241 tttgctcatg ttcctggcac agaagtttcc ggattttata gcattcaaca acacataccg
     3301 actgctttgg atagtgggag ggctcatcct agcaggaatt attatttcat tcatcataac
     3361 atggtttgtg attaatcgtt atattggcag aaaaattgaa tcattgtatt aattgaatat
     3421 aatatgagta agagtaatcc aaaaaaagta gtaataagta atactccaag agcaaaagtt
     3481 gaaacacagg gtagccgaaa atcgtcatca tttttcgggt cgaatgaaga gttgattttt
     3541 aataaagaaa acttcaaatg gacaggtatc agcctgttac tgatattcat agggatgctt
     3601 ctgatgactg gtggctccat gccaaaccca gatacttggg acccaaacat catttattct
     3661 gcgagaagga cagtactggc acccattttt atactcggcg ggttggtatt gcagatctat
     3721 gccatattta agtaaaatac gtttgtacac gaagcatatt ttatgacatt tatccaggca
     3781 atcattattg ctataataga aggtttgacg gagtttttgc ctgtgtcatc taccggacac
     3841 atggtcatag tatcatccat catgggtata gctgctgata actttgtcaa actctatgaa
     3901 atagtaatcc aattggcggc tatacttgca gtggttgtat attattggaa gaaatttttt
     3961 gatttcacca aaattcgttt ttacctgctt ctgatactgg caacgattcc ttcacttgtg
     4021 gtgggatatt ttttaaagag ccatgttgaa aaaatgcttg agagcccgct tgtgatagcg
     4081 tgcatacttt tacttggtgg cgtcgtattg ttatttattg ataaattttt tactcaaaat
     4141 accattcaga ctgataccaa aatgacaacc ggcaatgcag tttttattgg tttgtttcag
     4201 acattatcca ttttgttgcc aggccttagc aggagtgcag ccaccatcat aggcggtatg
     4261 cagcaagggt tgacaagaca gtttgcagct gagttttcgt tttttctggc tgtgcctacc
     4321 atgtgtgcag ctaccgtcaa gagtctgtat gatgtttata aaaatacacc tgaagtgcta
     4381 aacacggata accttgacat attgcttgtc gggaatgtgg tatcttttgt agtagccttc
     4441 atggccatta aatattttat tgaatatttg acgaagcacg gcttccggat tttcggtatt
     4501 taccgcatca ttctgggtgc acttatactt atccttcatt ttgcaggcgt aaaattattc
     4561 gtacattgat ggcacacagt actactccaa ttcttgaaga atttctggaa tcgcaggtac
     4621 tcctgatcga taaacccctt gactggacat catttgatgt ggtaaacagg ctaaggatag
     4681 cattgtgccg taagctgaag gttaagaagc ttaaagtagg tcatgcgggc acacttgatc
     4741 caaaggctac cgggttgctg atcatctgta ccggcaaagc taccaaacgc atcaccgaat
     4801 ttcaggatca ggcaaagact tatacaggtc agatattctt gggtgcgacc cggccttcgt
     4861 atgatttgga aacagaggtt gatgcacaat accctacaga tcacatttcg gatgaaatga
     4921 tccaatacac aactcaatca tttgtcggca tacaacaaca gatgccacct gtatattcgg
     4981 ccatcaagca agacggcaaa cctgtatata aaaaagcaca taaaggagaa ggtgataccg
     5041 tagtgatgca accacgcgaa gtaattattt acgatttcaa cacatcttct gatgctttac
     5101 cacttgtgtc atttgaagtg aaatgtagta aaggcaccta tattcgttca cttgcctttg
     5161 attttggtaa aaaaatcggt accggtgcat atttggcgag cctgagacgg acagccagtg
     5221 gagaatttac agtggatgat gcatggcaac tcgacgatct tattcgttac attgaccggt
     5281 acgagccttc ggttgttgtc cgaacttaag tcttacgttg ctttttcttc gcagccggga
     5341 ataaaatatt attaagaatt agcctataac cgggcgaatt ggggtgcagg tcaaggtctg
     5401 taggggggtc gttaaccctg tgctggtaat cttccgggtc gtg
//
LOCUS       DAOQQY010000195         5411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6761_length_5411_cov_4.245332, whole genome shotgun sequence.
ACCESSION   DAOQQY010000195 DAOQQY010000000
VERSION     DAOQQY010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6761_length_5411_cov_4.245332"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLE23_09305"
     CDS             <1..1115
                     /locus_tag="PLE23_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HMX83501.1"
                     /translation="KDAHPHNIDSTDLQFAFAKSSNTGISNLVVEHYGDNQAGKFIEK
                     LKQFGLKDKTGIEIPGEVPPTIKEAYSSVDLWSGTTLPWMSIGYELAITPLQLLTFYN
                     AIANDGKMMKPYLVSEIREYNKDRVKKSFEPQVLKKHIASRKSVRVIRELLEAVVDEG
                     GTAMNLRSSDYKIAGKTGTSQMNYQKINNTSNVGHRSSFVGYFPADKPMYSMVVVVSE
                     PKQNGYYGAYVAGPVFKNIADRIFSRMYDEHIAYNEVKPDKEELKKNKPGLVTAGFQS
                     DVSYLLDKMDVDFEEDVNTDWVKTSVEADKITLADKKIDPNKVPNVIGMGLRDATYLL
                     ERMGLKVEVVGCGKVKRQSISPGTLVRRQVVKLFLG"
     gene            1127..2602
                     /locus_tag="PLE23_09310"
     CDS             1127..2602
                     /locus_tag="PLE23_09310"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMX83502.1"
                     /translation="MQLLGRLLENISPFSIRGNTDCRIENICIDSRKVSKSSLFIAIK
                     GTLTDGHKFIESAIRQGAVAVVCESLPDTLVADVCYIVTDNSHKASGLLAAAFYGFPS
                     DSFKVIGITGTNGKTTVATLLHQLFSGLGYKCGLLSTVNNIIGSEVKASTHTTPEPIS
                     LQNLFAEMVDAGCDYCFMEVSSHAVDQYRIEGVKFYGAVFTNLTHDHLDYHKTFENYL
                     KAKKKFFDNLDKHSFALVNADDKNGRVMVQNTRASIKTYGLRTMADFKGKVIQNSVEG
                     LHLEINNVQMHSSLIGEFNAYNLLAVYGAAVLCGMDEYEAMLGLSSLKPAEGRFEIVK
                     DVRTRRTGIVDYSHTPDALEKILQTIKDIRPKTARILLVVGCGGDRDKTKRPVMAHIG
                     VVNSDVVILTSDNPRSEDPEVILDEMWKGIDTEYQPRAVRVTDRKQAIKMACLMSDND
                     DIIVVAGKGHEKYQEIKGEKFPFDDLEILKQEMNENIKIKK"
     gene            2713..3966
                     /gene="mraY"
                     /locus_tag="PLE23_09315"
     CDS             2713..3966
                     /gene="mraY"
                     /locus_tag="PLE23_09315"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMX83503.1"
                     /translation="MLYLIAKYLQTLYHIPGLRLFQYITFRAGLAIVLSLIISIFFGK
                     RIVNWLRRLQIGESVRDLGLTGQKEKEGTPTMGGVIIILAIMVPCLLLCDLTNVYILL
                     MLFATTWMAIIGFVDDYIKVFRKKKDGLDGKFKIAGQIGLGLIVALTMLFNEEVTVRM
                     EKEKAENLGYHIVKRTVVEDHRNQGTFKDMVYVKTTLTNVPFMKGNQFEYGDLLPSSM
                     REKWVWLLFTLIVIFIITAVSNAANLTDGLDGLATGISAIIGGTLGVLAYVSSNTIFA
                     DYLNIFYLPATSELVIFCACFIGACIGFLWYNAYPATVFMGDTGSLTLGGIIAALAII
                     LRKELLIPVLCGVFFVETLSVMLQVGYFKYTKKVSGEGKRIFLMSPLHHHYQKKGYHE
                     AKIVTRFWVVAVMLAVLTIITLKLR"
     gene            3994..5310
                     /gene="murD"
                     /locus_tag="PLE23_09320"
     CDS             3994..5310
                     /gene="murD"
                     /locus_tag="PLE23_09320"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMX83504.1"
                     /translation="MKIVILGGGESGVGSAVLAHSLGHDVFLSDGGMLRDDYKHTLES
                     LGIPFEEGGHQFENLADATLIVKSPGIADNAAIVKKLNELEIPTISEIEFGYRHTDAK
                     IIAITGSNGKTTTTSLVYHILNQAGFNVALGGNIGKSFAALVAEGGYDYYVLEISSFQ
                     LDGIQDFRPDIAILLNITPDHLDRYDYKFENYIASKFRITENQTNKDLMIYWAEDPVI
                     ASNSQFFNNITKKKGIKGPFVKDGIFSIGDFACQADNISIKGPHNEINAACAVTAALD
                     LGITDEVIANALATFVNMPHRLEYVATIGGVSFINDSKATNVDAAWYGLSAVNNPVIW
                     IVGGTDKGNDYTPLTEQVKNKVKAIVCMGADNSKIIDFFSNIVETIKDTHSAEEAVDI
                     AFSLANQGDTVLLSPACASFDLFNNYEHRGETFKKAVKNLIIKIES"
BASE COUNT         1713 a          944 c         1225 g         1529 t
ORIGIN      
        1 tgaaggatgc acatccgcac aatatagatt caactgatct acagtttgct tttgccaaat
       61 cttccaatac aggtatatca aacctcgttg ttgaacatta tggtgataat caggccggaa
      121 aatttataga aaaactgaag caattcggat taaaagataa aacaggtatt gaaataccgg
      181 gtgaagtacc acctaccatc aaagaagcat acagctctgt tgatctctgg agtggaacca
      241 ctttgccctg gatgtcaata ggttacgaac tagcaattac acctttacaa cttcttacat
      301 tttacaatgc catcgctaac gatggtaaaa tgatgaaacc ctaccttgta tctgagatca
      361 gagaatacaa caaagatcgt gtgaaaaagt cttttgagcc tcaggtatta aaaaaacaca
      421 tcgcctccag aaagtctgtc agagtaatca gggagctact ggaggcagtt gttgatgaag
      481 gaggtacagc aatgaatctt cggtcaagtg attataagat agccggcaaa accggtacct
      541 cgcaaatgaa ctatcagaaa atcaacaata catccaatgt tgggcataga tctagtttcg
      601 taggttattt tcctgccgat aaacccatgt attcgatggt agttgttgtg tcagaaccaa
      661 agcaaaacgg gtattatggt gcttatgtag ccggccctgt gtttaaaaat atagccgaca
      721 gaatattcag cagaatgtac gacgagcaca tagcatacaa cgaagtaaaa cccgataagg
      781 aagaattgaa aaagaataaa cctgggttgg tcacagccgg atttcagtcg gatgtttcat
      841 atctgctaga taagatggat gttgattttg aagaagatgt taatactgat tgggtcaaaa
      901 cttcagtcga agcagataag attacgcttg ccgataagaa aattgacccg aataaagtac
      961 ctaatgtaat tggtatgggg cttcgtgatg ctacctatct ccttgaaaga atgggtctta
     1021 aggtagaagt agtagggtgt ggaaaggtga agagacagag tatatctcca ggcacgcttg
     1081 ttcgcaggca ggtggtcaaa ttgttcttag gttaaattgc tgtaaagtgc agttattagg
     1141 cagattactc gaaaatatta gccctttttc aatcagaggt aatactgatt gcaggattga
     1201 aaatatttgt atagacagcc gaaaggtgtc aaaaagttct ttgtttattg ctataaaagg
     1261 tacactcacc gatggccata agtttattga gtcagctata cgtcaagggg cagtggcagt
     1321 tgtttgcgaa tccttaccgg atacattggt agcagatgtg tgttatatcg taactgataa
     1381 ttcgcataaa gcctccggtt tgcttgcagc cgctttctat ggatttcctt ctgattcttt
     1441 caaagtaatc ggcattaccg gtaccaacgg aaagacaacc gtagcaacac tgcttcatca
     1501 attattttct ggtttgggtt acaaatgcgg gttgctttct acagtgaata atataatagg
     1561 aagtgaagta aaagcttcga ctcatactac acccgaacct ataagccttc aaaacctgtt
     1621 cgctgagatg gttgatgcag ggtgcgatta ctgctttatg gaagtgagtt cgcatgctgt
     1681 tgatcagtac cgtattgagg gagtaaaatt ctatggagcg gtatttacta atcttacgca
     1741 cgaccatcta gattatcaca aaacattcga aaactatcta aaggcaaaga aaaagttctt
     1801 cgataatctg gataagcatt catttgccct tgtcaatgca gatgataaaa acggaagagt
     1861 gatggttcag aatactcgtg cttcgatcaa aacttacgga ttgcgtacaa tggctgattt
     1921 caaaggaaag gtcatacaga attcggttga gggtttgcac cttgagatca acaatgttca
     1981 gatgcatagt agtcttatcg gtgaatttaa tgcttacaat cttcttgccg tatatggtgc
     2041 tgctgtcttg tgtggaatgg atgagtacga ggcgatgctg ggattaagca gtttgaagcc
     2101 tgccgaaggt agattcgaaa tagtgaaaga tgtacgtacc cggcggacag gaatagttga
     2161 ttattcgcac acaccagatg cgttggagaa aattttgcaa accattaaag atatcagacc
     2221 caaaacagcc agaatacttc tcgtagtggg gtgtggtgga gaccgcgaca agactaaacg
     2281 accagtgatg gcacatatag gtgtagtgaa tagtgatgtg gttatactta ccagtgacaa
     2341 cccaaggagc gaagaccctg aagtgatact tgatgaaatg tggaaaggca tagacaccga
     2401 atatcaacca agagctgtga gagtaacaga ccgtaaacaa gcaataaaaa tggcttgcct
     2461 aatgtccgac aatgacgata tcatcgttgt agcaggcaaa gggcatgaaa aatatcagga
     2521 gataaaagga gaaaagtttc cttttgatga tcttgaaatt ttaaaacaag aaatgaatga
     2581 aaacattaaa attaagaaat aaagacttta tcattctcag atcaattgta tgcagctgcc
     2641 ggtggactta gtcctattaa gtccattgga gctgattttg agaacagaat gaaagattta
     2701 agataaaccg atatgttata cctgattgcc aaatatttac aaaccctgta ccacatccct
     2761 ggattgaggc tgtttcagta catcaccttc agggctggtt tggcaatcgt attatcactt
     2821 atcatctcta ttttttttgg taaaagaatt gtcaactggc tgcggaggtt gcagataggt
     2881 gagtcggtcc gagacctagg tcttaccggt caaaaagaaa aggaaggtac acctaccatg
     2941 ggtggtgtta ttattatcct tgccattatg gttccatgcc tattgctatg tgaccttact
     3001 aatgtatata tactgctgat gctttttgct actacatgga tggccattat tggttttgta
     3061 gatgattata taaaggtatt caggaaaaag aaagatggtc ttgacggtaa attcaagata
     3121 gccggacaga taggcctggg cctcattgta gcacttacca tgctgttcaa cgaagaggtg
     3181 accgtaagaa tggaaaaaga gaaagccgaa aatctgggat atcacatagt gaagagaacc
     3241 gttgtagaag atcatcgcaa ccaaggcacc ttcaaagata tggtttatgt aaaaactaca
     3301 ctgaccaatg tgccattcat gaaggggaac cagtttgaat acggagatct gttgccttca
     3361 tctatgcgcg agaaatgggt ttggttgtta tttaccctaa tagtgatatt cattattact
     3421 gcggtgtcaa atgctgctaa tcttacagac gggctcgatg ggcttgctac cggtatatct
     3481 gctattattg gcggtacgtt gggtgtgctg gcttacgttt cgtctaatac tatttttgca
     3541 gactatctga atatatttta cctgccggcc acaagtgagc ttgttatatt ttgtgcctgt
     3601 ttcattggag catgtatcgg gtttttgtgg tacaatgctt atccggctac ggtgtttatg
     3661 ggtgataccg gaagcctcac gcttggtggt atcattgcag cacttgccat catattgcgc
     3721 aaggaattac tgatacccgt attgtgtgga gtatttttcg tagagacatt gtctgtaatg
     3781 ttgcaggtag gttattttaa atatacaaaa aaagttagtg gtgaaggaaa acggattttc
     3841 ctcatgtcgc cattacacca tcattatcaa aagaaaggat accatgaagc aaagattgta
     3901 acacgcttct gggtggtggc cgtaatgcta gcagtgctca ccatcataac attaaaattg
     3961 agatagaata aacattgcat aaagatctga ataatgaaaa ttgtgatact tggtggcggt
     4021 gaaagtggtg tagggagtgc tgttttggca cattcactag gtcatgatgt ttttttatct
     4081 gatggaggaa tgctcagaga tgattataag catactctcg aatcacttgg tatccctttt
     4141 gaagaaggtg gacaccagtt tgaaaacctc gctgatgcca cactaatagt gaaaagcccg
     4201 ggtatagcag ataatgcagc aatagtcaaa aagttgaacg agcttgagat tccgactatt
     4261 tctgagatag aatttggcta ccgacatacg gatgccaaga taattgccat cacaggtagc
     4321 aacggtaaga ccaccactac ttcgcttgtt tatcatatat tgaatcaggc aggttttaat
     4381 gttgctcttg ggggcaatat cgggaagagc tttgccgcat tagtagctga aggtggctac
     4441 gattattatg tgttggagat cagtagtttt caattagatg gtattcagga ttttagaccg
     4501 gatattgcta tactattgaa cataacacct gaccatcttg acagatatga ttacaaattc
     4561 gaaaattata tcgcatcaaa gttcaggatt actgaaaacc aaaccaataa ggatttgatg
     4621 atatattggg cggaggatcc ggtcattgct tcaaattctc aatttttcaa caacatcacg
     4681 aaaaaaaagg gtattaaagg accttttgta aaagatggga ttttcagcat tggagatttt
     4741 gcatgccaag cggataatat ttcaataaaa ggcccacata atgaaataaa tgcagcctgt
     4801 gcagtaactg cagcacttga cctagggatc acagatgagg tgatagcaaa cgcattggct
     4861 actttcgtaa atatgccaca ccgacttgaa tatgttgcaa caataggagg tgtaagtttt
     4921 atcaacgaca gtaaagccac caatgtagat gcggcttggt atggactcag tgcagttaat
     4981 aatcctgtaa tctggatagt cggagggaca gataaaggca atgattacac acctttgacc
     5041 gagcaggtaa aaaataaagt aaaagccatt gtctgtatgg gagcagacaa cagcaaaatc
     5101 atagattttt tcagcaatat tgttgaaact atcaaagaca cacactcagc agaagaagca
     5161 gttgatatag ctttctcgct ggccaatcag ggcgatacag tattgctttc accggcatgc
     5221 gcaagctttg acctttttaa taattacgaa catcgcggag aaacctttaa gaaagcagtg
     5281 aaaaatttaa ttattaaaat tgaaagttga taaagtatgg tagccagagc attaaaaagc
     5341 atttataacg gcgaaagagg tgaccgcatg atttggttta taatcatcgc actcggtatg
     5401 gttagtttgt t
//
LOCUS       DAOQQY010000196         5375 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6849_length_5375_cov_5.330075, whole genome shotgun sequence.
ACCESSION   DAOQQY010000196 DAOQQY010000000
VERSION     DAOQQY010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5375
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6849_length_5375_cov_5.330075"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLE23_09325"
     CDS             <1..736
                     /locus_tag="PLE23_09325"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83505.1"
                     /translation="NPNTIIGNGPEDREYVGDMNFITTDRVLTEGSTINVDFNTEKFN
                     DILAYQFGMDYNKEYLELTGTDAGVLPGITKSSFGLNFDTDGIITSAWYNATAQNLSS
                     SDKAFRLSFKVKKGGKKLSQVLKLDNTKIQTVGYDKEYSPVNLGLKFKSDAVTNETYS
                     FELFQNQPNPFKRTTVISFTLPEAESGQIEISDLKGKTIKLIEKNFEKGLNREEVTLD
                     NIPSGVLFYKVKTETLSAVKKMVIIE"
     gene            998..>5375
                     /locus_tag="PLE23_09330"
     CDS             998..>5375
                     /locus_tag="PLE23_09330"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMX83506.1"
                     /translation="MLRKRTLSTAHRTRILISSMILGIFVWFVSGVSAQNATIQIPDI
                     SAQCGDTITVPVQVGVKDFIGYTGIQFSLTWNHDALDYIGLTGQYGPLDLKNSNFKLP
                     PAFPNKDTLAFVWWLSVTPLDIEPNTGLFSIKYVVKGAGPINSLINIINKPTPIFIYG
                     EVGGNPFTLIPNVNTDPGSFKINDQNIPILVCPDDVTVVAPAGSGKAIANNIYPKQAS
                     DNCHVAFLGYSFSGSTTGFGQGDPSGTVFNAGSTLVNYTINDPSNNFNYCQFEVFVVD
                     TASSILEVKLQNIQTDCSKDKVLVNVTADNFNDIAGLTFGINWDENIFQLDTIINLNT
                     TLSLVPLGKVNSSFGPTPKDNDTLTFFMPVNPGGITLPTGDTLYTLVFNKIGAATCSK
                     INFISTPTVPIKAFAAGTPPFEVPIDTANSQICFNDTIPPTIVCPADTLVKVNQGQFS
                     AIVGGLTPTTGDNCGIKELYYTLSGSTSGSGTGDVSNFTFFYGQTEVNYTVQDFSGNT
                     ASCNYKIVVADSVLRLLPTSTIAICDSGKVAINVTASNFKDILSLQCAMAWNPSILSF
                     DTVGNFDPNLQLNFGSNFGPANITDTLNFAWIADTNGLSLPDGTTLFTVYFDINANAG
                     SQTKFEFVSSSTTPIEAYTTKGQIGVLAFDALINIFDFKPPTLECPSNINIKIPQGTT
                     FITINDIAPINVSDNCGIKSVKYETTGSTTVNGADDASGNVFNLGSTTVTYIVEDFGG
                     NTETCSFVINVVPDTFLLYLDTINAQCTNGVVTVDVKVKNFNNITSLQFGMTWDKLLL
                     ALDTITNLNPALSLIGQGLPGTNFGPPVMTDSLTFAWYNNSGVTLPDGSVLFTLTLTP
                     KQIKILNTFVNFINYPTTPIEVSATNGGIPFLIPYKIINGVVNIVDSIPPVLTCPPNI
                     TVFTDLGKCSATAVWSTVTAVDNCSALVNLTSNYDPGAEFKLGIDTVIYTGTDEFGNV
                     GTCSFEIKVIDGEKPVINCPGNQTYDNDPGECGSTIALPQTIDATDNCSSTLTYKVNQ
                     PDIKFYSVGGHNILYTVTDEAGNSATCNFILIIKDLELPTIVDCPIDTVVEPAVNINN
                     MCFAEVSWSEPLVDDNCPNTQVKSSVTSGSLFPAGTTIVQYIVNDASGNTATCSFSVT
                     VQSFASGFNNCPSDIVVPVQQDTCGAFVSWVPPFFVDACGDTLNVYSNYSPGTFFPPG
                     TTQVVYITAANSIVLDTCYFNVTVEDEDPQVINCPGDIIISSLPDTCGNFVDWIKPFV
                     LNSCGDSLTLTSNYTPGAFLPVGVYSVVYTAPNLPGCAFNITVTDAIKPEHGLCPPNQ
                     MIEVPDTICGAIVTWSEPEFTDNCDNDLFIISSHNSGDYFPTGKTIVTYIAIDDFGNV
                     DTCSFELFIRETIKPVLFNCPSDVTLESLPNACGNFHNWIVPTATDNCDTNVVITSNF
                     SPGEFFPVGTTVVIYTATDNSGN"
BASE COUNT         1654 a         1062 c         1049 g         1610 t
ORIGIN      
        1 aaacccgaat acaatcatag gcaatggacc tgaagacaga gagtatgtag gtgacatgaa
       61 ttttattact actgaccgtg tacttacaga aggttctacc atcaatgttg atttcaacac
      121 tgaaaaattc aacgacatcc ttgcatacca atttggaatg gactacaaca aggaatatct
      181 cgaattgacc ggaactgatg caggtgtatt acctggtatc acgaaatcat cattcggatt
      241 gaattttgat acagacggta ttatcacttc agcatggtat aatgctactg ctcaaaactt
      301 aagtagctct gataaggcat tcagactaag cttcaaagtg aagaaaggcg gcaagaagct
      361 tagccaggtt ctgaagcttg acaatactaa gattcagaca gtaggttatg acaaagaata
      421 cagccctgtt aaccttggat tgaaattcaa gtcggacgct gttacaaacg aaacatacag
      481 tttcgaattg ttccagaacc agccgaatcc gttcaagcgt actacagtga tttctttcac
      541 attgcctgag gcagaaagtg gacaaatcga gatttcagat ttgaaaggaa aaactatcaa
      601 attaattgag aagaattttg aaaaaggttt gaaccgtgaa gaagtaacat tggataatat
      661 tccatcaggt gtactcttct acaaagtaaa aacagagaca ctttcagcag ttaagaaaat
      721 ggtcattatt gaataatgaa tgaataagcc aacaagtcgg gagtaatcta gctcctgact
      781 tgtctggttt aagtatgaaa atactgagta aaaccacaaa gaatcctaaa ataccgtaac
      841 aactgaccca ccctattaca gagtccctga attagttttc cgattaattt ttaagcaaaa
      901 tcatcgctta ataaattgct atatctgaaa acattacaaa taatcagcaa ttataattat
      961 ctaaaatagt gaacattata tattcaaaga acgcgtaatg ttaagaaaaa gaacactttc
     1021 aacagcccat agaaccagaa tcctgatatc ctcgatgata ttaggcatat ttgtttggtt
     1081 cgtgtccggt gtctcagctc aaaatgcaac cattcagata cctgatattt ccgcccaatg
     1141 tggtgacacc attacggtac cagttcaggt tggggtcaag gattttattg gttataccgg
     1201 tattcaattt tcattgacct ggaatcatga tgctttggat tacattggtc ttaccgggca
     1261 gtatggtcca cttgacttaa aaaactctaa cttcaagctg ccaccggctt tcccaaacaa
     1321 agacacgctg gcctttgttt ggtggttaag tgttacacca ttggacatag aacctaacac
     1381 cggattgttt tcgataaaat atgttgtcaa aggtgccggt ccaatcaact ccttaatcaa
     1441 cattatcaac aagccgacgc caatatttat atatggtgaa gttggtggca acccatttac
     1501 tttgattcct aatgtgaata cagatccggg ctctttcaaa ataaatgatc agaacattcc
     1561 aattttggtt tgccctgatg atgtaactgt tgtagcaccg gcaggaagtg gtaaagccat
     1621 cgctaacaac atttacccaa aacaggcatc cgataactgt catgttgctt ttcttggata
     1681 ttcattcagt ggctctacaa ccggatttgg acagggagac ccaagtggta ctgtattcaa
     1741 tgcaggtagc acattggtaa attatacaat caatgaccca agcaataatt tcaattattg
     1801 ccagtttgaa gtgttcgtag tggacactgc ctcttcaata ctcgaagtaa aattgcaaaa
     1861 catacaaacc gattgttcga aagacaaggt actggtaaat gtaacagcag ataacttcaa
     1921 tgatattgcc ggcctcactt ttggaatcaa ttgggatgaa aacatttttc aacttgatac
     1981 catcattaat ctgaatacta cactttctct tgtaccgctt ggtaaggtta attcaagttt
     2041 tggccctact ccgaaggaca atgatacttt aacatttttt atgcctgtga atccgggtgg
     2101 tatcacattg cctacaggag atacacttta tacacttgtt ttcaataaaa ttggagctgc
     2161 cacttgttca aaaattaact ttattagcac accaaccgtt ccaataaaag catttgcagc
     2221 aggtacaccg ccttttgagg taccgattga tactgccaat agtcagatat gcttcaatga
     2281 caccattcct ccaactattg tttgcccggc tgataccctc gttaaagtaa accagggtca
     2341 attctcagct attgtaggtg gacttacacc tacaacagga gacaactgtg gtataaaaga
     2401 gctatattat acactgagtg gttctacatc cggctcagga accggagatg taagtaattt
     2461 tacatttttc tatggccaga ctgaagtaaa ttatacagtt caggattttt caggaaatac
     2521 tgcgagttgc aattataaga ttgtagtagc ggattcggta ttgcgtttat tgccgacatc
     2581 tactatagct atttgtgata gtggcaaagt ggccataaat gtaactgcaa gtaatttcaa
     2641 agatatttta tcactgcaat gtgctatggc gtggaatcca tccatattaa gctttgacac
     2701 cgttggaaat tttgatccga acctacaatt gaatttcggc agcaattttg gtcctgccaa
     2761 tattaccgat acacttaatt ttgcctggat agcagatacc aacgggttga gccttccgga
     2821 tggcacaaca ttgtttacgg tttattttga tataaatgcc aatgctggct cgcagactaa
     2881 attcgaattt gtgtcttcct ccacaacacc tatagaggca tacactacaa aagggcagat
     2941 tggcgtattg gcttttgatg cactgattaa tatatttgac tttaagccac ctaccctcga
     3001 gtgtccttcg aatataaata ttaagattcc gcagggtacc acatttatta ctattaatga
     3061 cattgctccg ataaatgtat cagacaattg tggtataaag agtgttaaat atgaaacaac
     3121 aggtagtaca acagtcaatg gtgctgatga tgcaagtggt aatgtcttta atcttggcag
     3181 cactacagtg acctatatag ttgaagattt tggaggaaat accgaaacat gctcatttgt
     3241 catcaatgtt gttcctgaca cattcctcct ttacctggat accattaatg cccagtgtac
     3301 gaatggtgta gtcacagtag atgtaaaagt aaagaatttt aacaacatta caagtctgca
     3361 gtttggaatg acttgggata aattgttgct tgcattagat accatcacca atttgaatcc
     3421 tgcattgagt ctgataggtc agggtctgcc cggaacaaat tttggacctc cggtcatgac
     3481 tgattcactt acatttgctt ggtacaataa tagtggtgta acccttcctg atggctccgt
     3541 gctatttaca ctgacactga caccaaaaca aataaagata ctgaatactt ttgtcaactt
     3601 tatcaattac ccaacgaccc cgattgaagt atctgctacc aatggtggca taccattcct
     3661 cattccttac aagataatca atggtgttgt aaatattgtt gacagcatac ctcctgtgct
     3721 tacgtgcccg cctaatatta ctgtatttac ggatctgggc aaatgttcag ctacagccgt
     3781 atggtcaaca gtaacagcag ttgataattg ttctgcatta gtgaacctca cttcaaacta
     3841 cgatccgggt gctgagttca aactcggtat agatactgtt atttacaccg gaacggatga
     3901 attcggaaat gtgggcacat gtagttttga gataaaagtg atagatggtg aaaagcctgt
     3961 gatcaactgt cctggcaacc agacttatga caatgacccg ggagaatgcg gttctacaat
     4021 tgcattgcct cagacgattg acgctacaga taattgttca tcaacactga cttacaaagt
     4081 caatcaacct gatattaagt tttactcggt tggtggtcat aacattttat acactgtgac
     4141 agatgaagcc ggcaattcgg cgacatgtaa ttttattctg attatcaagg atcttgagtt
     4201 accaaccatt gtagattgtc cgattgatac cgtcgtagag ccggcagtta acatcaacaa
     4261 tatgtgtttt gctgaagtaa gttggagtga acctttggta gatgataact gtccgaacac
     4321 acaggttaaa agcagtgtga catccggaag tctcttcccg gctggtacta cgattgtaca
     4381 atatatcgta aatgatgcat caggtaatac tgctacatgt agctttagtg ttactgtaca
     4441 atcatttgca tcaggtttca acaattgtcc atcagatatt gtagtacctg tacagcagga
     4501 cacatgcggg gcctttgtaa gctgggtgcc accattcttt gttgatgcat gtggtgacac
     4561 cttaaatgta tattcaaact acagtcccgg tacatttttc ccaccgggta ctacacaagt
     4621 tgtttatata acagctgcaa actctatagt gcttgataca tgctacttta acgtaacggt
     4681 agaggacgaa gacccacagg tcatcaattg tccgggtgat atcattatat cttccttacc
     4741 tgatacatgt ggcaattttg ttgactggat taagccattc gtgctgaata gctgtggcga
     4801 cagtttgaca cttacttcta attatactcc gggtgcgttt ttaccggtgg gtgtttattc
     4861 ggttgtttat acagcaccca atctaccggg atgtgcattt aacattaccg ttactgatgc
     4921 cataaagcct gaacacggac tttgtccacc aaatcagatg atagaagtac ctgatacaat
     4981 atgtggtgct attgtcacgt ggtcagaacc agagttcaca gataattgtg ataatgattt
     5041 gtttatcatt agcagccaca attcaggaga ctacttccct accggaaaga ccattgtcac
     5101 ttatatagca atagatgatt ttggaaatgt tgacacttgt agtttcgaat tatttataag
     5161 ggagactatc aaaccggtat tatttaattg cccttcagat gtaacattgg aatcgctgcc
     5221 aaatgcttgt ggaaatttcc ataactggat agtacctact gctacggaca attgcgacac
     5281 gaatgtagtt atcacatcta atttttcacc aggtgaattt ttccctgtcg gtacaacagt
     5341 tgttatatac acagcgactg acaattcagg taatg
//
LOCUS       DAOQQY010000197         5369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6864_length_5369_cov_4.541212, whole genome shotgun sequence.
ACCESSION   DAOQQY010000197 DAOQQY010000000
VERSION     DAOQQY010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6864_length_5369_cov_4.541212"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /gene="pruA"
                     /locus_tag="PLE23_09335"
     CDS             <1..1247
                     /gene="pruA"
                     /locus_tag="PLE23_09335"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HMX83507.1"
                     /translation="QSKNVFQAEIDCVAELCDFFRFNAQYMQEIYHQQPESPRGQWNQ
                     LEHRPLEGFVFALTPFNFTSIAGNLPSAPAMLGNVVVWKPANTQVLSAMVIMEVLEAA
                     GLPPGVINLVFVDGSVIGDVVFSHPQFAGLHFTGSTGVFQNIWETIGKNIRIYKSYPR
                     IVGETGGKDFVIAHPSARAKEVATALSRGAFEFQGQKCSAASRAYIPKSLWPEVSDML
                     KADLADFKMGSPEDFRNFINAVIDEKAFDKITTHIANAKKDAQCRIIAGGNFDKSKGY
                     FIEPTVIQTSDPRYVTMCEELFGPVLTVYVYDDNEFEKTLDILDETSEYALTGAIFGQ
                     DRYAVEMACRKLRHSAGNFYINDKPTGAVVGQQPFGGARASGTNDKAGSILNLLRWVS
                     PRTIKENFVPPVDYKYPFLQEE"
     gene            complement(1356..2861)
                     /locus_tag="PLE23_09340"
     CDS             complement(1356..2861)
                     /locus_tag="PLE23_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX83508.1"
                     /translation="MKNILKLSVVLLFTFLIQHSSIAQHLGAPNALHGKVLLMDHFTL
                     IDADSTPYKGYTYGLELGYSRAITNWLNFGIPVKFGLIQLPDDPRKKLFSSIDFVLRL
                     QAFKPNNLLTPFLFAGGGVVNEDLTTTAIQFPLGAGLNIRLTPREYLTVSAEYRRSLA
                     DNRDNLQFGLGALLMLGKSDPKAMDKDGDGVPDNEDLCPDKPGSFVTRGCPDKDGDGI
                     ADKDDKCPDVAGTPEFMGCPEPKDTDGDGIIDSEDQCPDLAGPVATKGCPDRDGDGIA
                     DKDDKCPDVAGTRENNGCPTTIDSDGDGVPDEADKCPTLPGLAKYNGCPDSDGDGIPD
                     QEDDCPTLPGLVQYKGCPDSDGDGVHDKIDKCPYAAGPASNNGCPEIKEEVKSALKKA
                     TQAVRFKTGTAELEITSYKTLNEVAKILNENPVYSMEIAGHTDNVGKDEKNLKLSEAR
                     AKACYDYLLAQGIDAGRLVYKGYGKTLPVATNSTAEGRALNRRTEFNLFLK"
     gene            complement(2962..4530)
                     /locus_tag="PLE23_09345"
     CDS             complement(2962..4530)
                     /locus_tag="PLE23_09345"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMX83509.1"
                     /translation="MRYWILCFFAFAACGLRAQNFYDINAIREVKIQFEDNDWDKKLV
                     SLRNTKKKSRLTGNITIDGKKYEQVGVRYKGNSSYNATRKEEKAKLPLNVESDYKLKK
                     QDFGDKIETLKLSNMFRDPSYIREVLAYWLANQLVVSPSANFAKVTVNKTYLGVYTST
                     ESIDSRFLKNKFGDKNGIFFKCDPDWDSPTLKHCPKGDKASLMWQGPDSACYNGTYEL
                     KNKSGYKELLNLISILDKNPSNASEVLDVDKTLWMHAFNHVIVNLDSYTGSLSHNYYL
                     YRDSSNVFVPLLWDLNLAFGGFRLDGKKNLTDIELFEYPLLSHSEDPSRPLISKLLSI
                     PLYKKIYLAHCRYIYENFLVNDKLEKEAERLSSFIEPDVKKDVNNLYSFNDFKQNLDK
                     KVSIKNVPIVGVTELIRARKAYLQNFYSGIPKLKIDSVFYKQDSTGYNIYVKTEETAK
                     PFLFYKNEQHTRYNSVQMQEDKNLSDTLGGKCFVFHLPASEKTYYYVVLESEKVAQVF
                     PARASNEPLKIPEK"
     gene            complement(4536..>5369)
                     /locus_tag="PLE23_09350"
     CDS             complement(4536..>5369)
                     /locus_tag="PLE23_09350"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMX83510.1"
                     /translation="SSYSGKECQNYYLYKNNFGKFVPVIWDLNLAFGSYKNTKVGTSD
                     LTQKELQELDPLLHIDDIYKPLISRLLSIKENRMVYLAHIRQLFEEIVISDYLEKRAE
                     ELQKLIRKAYLDDPYKQYSVMEFDKSLTTTIGSKTKIPGIMELMQKRLKYLRKHDLLK
                     NLPSEVTNVTYRARQLYSNEQIQTFTITAETNNFPKKLVIYYRPDAKSDYQSATMYDD
                     GTNSDETAGDGKYTVVIDPKSMYNSMEYYIQTENTGAVGFYPNNYVLKPAVVTLAELN
                     K"
BASE COUNT         1547 a         1078 c         1022 g         1722 t
ORIGIN      
        1 gccaatcaaa gaatgtgttc caggctgaaa ttgattgtgt tgctgagtta tgcgactttt
       61 tcaggttcaa tgctcagtat atgcaagaga tttaccacca acaacctgaa agcccgcgcg
      121 gtcagtggaa tcaacttgaa catcgccctc ttgaaggttt cgtgttcgct ttgaccccat
      181 tcaattttac atctatagct ggcaatctgc caagtgcacc ggccatgctt ggtaatgtag
      241 tagtctggaa acctgccaat acacaggtgc tctctgcaat ggtcatcatg gaggtacttg
      301 aagctgcagg tttgccaccg ggggttatta atctggtatt tgtagatggt agtgtcattg
      361 gtgatgtagt atttagccat cctcagtttg caggcctaca ctttacaggt agtacaggtg
      421 tttttcagaa catctgggag acaataggca aaaatatccg tatctataaa tcatacccaa
      481 ggatagtagg cgaaacaggt ggtaaagact tcgtaatagc ccacccttca gcacgtgcaa
      541 aagaagtggc aacggcactg tccagaggcg cttttgagtt tcagggtcaa aagtgttcgg
      601 cagcatcgag agcttacatt cctaaaagct tatggcctga agtgagcgac atgctcaaag
      661 cagatcttgc tgattttaaa atgggatctc ctgaagattt tagaaatttt atcaatgctg
      721 tcattgacga aaaggctttt gacaaaatta caacccacat tgcaaatgct aagaaagacg
      781 cgcagtgccg aataatagca ggtggcaatt ttgataaatc aaaaggttac ttcattgagc
      841 cgactgttat tcagacttca gacccaaggt atgtgacaat gtgcgaagaa ttgttcgggc
      901 ctgttttgac cgtttatgta tatgatgaca atgaattcga aaaaacgctt gatattttag
      961 acgaaacatc tgaatatgct ttgacaggtg ccatcttcgg acaagacaga tatgctgtag
     1021 aaatggcatg cagaaaactc agacactctg cgggtaattt ctatataaat gataaaccaa
     1081 ccggagcagt cgtaggtcaa cagccttttg gtggtgcaag agcatccggc acaaatgaca
     1141 aggcaggttc tattcttaac cttttgagat gggtaagtcc taggacaatc aaggaaaatt
     1201 ttgttccacc agttgattac aaatatcctt ttttacagga ggaataattc aatttcctcc
     1261 tatttcagct tacaataggc caaacccacc ctctttaaca aaaaaaatag cctgcaaata
     1321 aagcaggcta tttttctatg gttcaaataa taccgttatt taaggaacag attaaactcc
     1381 gttcttctgt taagtgcacg accttcggca gtactattgg tagctacagg caatgtttta
     1441 ccataacctt tatataccag ccttccggcg tctatacctt gcgccagtag ataatcgtag
     1501 catgctttcg ctcttgcctc agaaagtttc aaattctttt catcctttcc tacgttatct
     1561 gtgtggcctg ctatttccat actgtatact ggattttcgt tcaatatttt tgctacttca
     1621 tttaatgttt tgtatgaagt aatttcaagt tcagccgtac ctgttttgaa tcttacagct
     1681 tgggtagcct ttttgagagc agacttcact tcttctttaa tctcagggca tccattgtta
     1741 gaagcaggac ctgcagcata cggacatttg tcaattttgt catggacacc atcaccgtca
     1801 gaatcaggac atcctttgta ttggacaagt ccaggtaaag taggacaatc atcttcttgg
     1861 tcaggaatgc catcaccatc tgaatccgga catccattat actttgcaag acccggcaat
     1921 gtaggacatt tatctgcttc atccggcaca ccatcaccgt ccgaatctat tgtagtcggg
     1981 catccattgt tctctcttgt gccggctaca tcagggcact tatcgtcttt atcagctata
     2041 ccatcaccgt ctctgtcagg gcaacctttg gtggctaccg gtccggccaa atcaggacat
     2101 tggtcttcac tatctattat accatctccg tctgtatctt taggttccgg gcaacccata
     2161 aattccggtg tgccggctac atcagggcat ttatcatcct tatcagctat accgtcacca
     2221 tctttatccg ggcagccacg tgtcacaaaa gagccaggtt tgtcaggaca caaatcttca
     2281 ttgtctggta caccgtctcc atctttgtcc atggcttttg gatcactctt accaagcatg
     2341 agtaaggctc caagcccaaa ctgcagatta tcgcggttat cagccaatga acgacgatac
     2401 tcagcgctta ctgttaaata ctctctaggt gtaagacgta tattaagtcc ggcacccaaa
     2461 ggaaattgga tagcagtagt agtaaggtct tcattaacga cgccaccgcc tgcaaacaaa
     2521 aaaggagtca gaagattgtt cggcttgaat gcctgcaacc tcagaacaaa atcaatcgaa
     2581 gaaaatagct tctttctagg atcgtcgggt agttggataa gaccgaattt tacagggatt
     2641 ccaaaattga gccaattggt tatggcacga ctatagccca attctagccc ataggtatat
     2701 cctttataag gtgtactatc agcgtctatt aaagtaaaat gatccatgag caatactttg
     2761 ccatgtaatg cattcggtgc tccaagatgt tgagcaatgg aagaatgctg gatcaaaaat
     2821 gtaaaaagaa gaactacact gagttttaag atattcttca tggaatacgg tttttgttgg
     2881 taaacagatt taataaggtg aaaaacaatt aataatatac taacaaatat attaccaaac
     2941 tttacaatat aatgtaaaaa atcacttttc aggtattttc aaaggctcgt ttgaggcccg
     3001 tgcagggaat acttgtgcta ctttttctga ttcaaggact acgtagtaat atgttttctc
     3061 ggatgcaggt aagtgaaaaa caaaacattt tccacccaag gtgtcagata aatttttgtc
     3121 ttcctgcatt tgtacggaat tgtaacgagt atgttgctca tttttataaa aaagaaatgg
     3181 cttagctgtt tcttccgttt ttacatatat attgtaacct gtagaatcct gtttataaaa
     3241 aactgaatca atttttaatt tgggtattcc ggaataaaaa ttctgcaaat atgcttttct
     3301 agctctaatc agttctgtta cacctactat gggtacattc ttaatgctta cctttttatc
     3361 aagattttgc ttaaagtcat taaatgaata tagattattg acatctttct ttacatccgg
     3421 ttctataaat gaactcaatc tttcggcttc tttttcgagc ttatcgttaa caagaaaatt
     3481 ttcgtagatg tacctacaat gcgccaaata aattttttta tataatggaa tgctaagcag
     3541 ctttgatatc agcggtctag agggatcttc gctgtgcgat agcaatggat attcaaaaag
     3601 ttcaatatca gtcaaatttt tctttccgtc aagcctaaac cctccgaatg cgagattgag
     3661 atcccacaac aatggcacaa aaacatttga tgaatcgcga tacaaataat agttatggct
     3721 cagactgcct gtataactgt caaggttgac tatcacgtga ttgaatgcat gcatccacaa
     3781 tgttttatca acatctagta cttcactagc gtttgaaggg tttttatcaa ggatagatat
     3841 taggttgagt agttctttgt aaccagattt attctttaat tcgtaagttc cgttataaca
     3901 agctgagtct ggtccttgcc acattaagga tgctttatca cccttcgggc aatgtttcaa
     3961 ggtaggtgaa tcccaatcag gatcacattt aaaaaatata ccgtttttgt caccaaattt
     4021 atttttaaga aaacgactat cgattgattc ggtgctagta tatactccaa gataagtttt
     4081 gttgacagta actttggcaa aattggctga aggtgatacc accagttggt tagcaagcca
     4141 ataagctaag acttcgcgga tataactggg gtcgcgaaac atgtttgaca atttcaatgt
     4201 ttcaatttta tcaccaaaat cttgtttttt tagcttataa tcggactcta catttagtgg
     4261 aagcttggct ttctcttcct ttcgtgttgc gttgtaagag ctattgccct tatatcttac
     4321 acctacctgt tcgtactttt taccgtcaat ggtgatatta cctgttagtc tgcttttctt
     4381 cttagtatta cggagactta caagtttctt atcccagtca ttgtcttcga actgaatctt
     4441 tacttctctg atggcattaa tgtcatagaa gttttgagct cgtagtccgc aagctgcaaa
     4501 ggcaaagaaa caaagtatcc aataacgcat tatccttatt tattcaactc agccaaggta
     4561 acaactgctg gctttaaaac ataattattc ggataaaatc caacagctcc tgtgttttcg
     4621 gtctgaatgt agtactccat actgttatac atacttttcg gatctattac tacagtatat
     4681 ttgccgtcac ctgctgtttc atcactatta gttccgtcat catacattgt ggctgattgg
     4741 tagtcagatt tagcatccgg tctgtagtaa ataacaagtt ttttaggaaa attattggtt
     4801 tcggctgtaa ttgtaaatgt ttgtatttgt tcatttgagt aaagctgacg tgctctgtaa
     4861 gtaacatttg taacttcaga aggcagattt tttagcaagt catgtttgcg caaatatttt
     4921 aatctttttt gcatcaactc catgatacct ggtattttgg ttttacttcc aatggttgta
     4981 gtaaggctct tatcaaattc catgaccgaa tattgcttgt atggatcatc cagatatgct
     5041 ttgcgaatca atttttggag ttcctctgcc ctcttttcaa gataatctga tataacaatt
     5101 tcttcaaata gctgacgaat atgagccaga tataccatac ggttttcttt aatactgagc
     5161 agacggctta tgagaggctt atagatatcg tcgatatgca gtagcggatc caactcctga
     5221 agctcttttt gtgttagatc tgaggtgcct accttagtat ttttataact gccgaaagcc
     5281 aggttcaaat cccagataac aggaacaaat tttccaaaat tatttttgta caaatagtag
     5341 ttctgacatt ccttacccga ataactgct
//
LOCUS       DAOQQY010000198         5359 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6886_length_5359_cov_5.314857, whole genome shotgun sequence.
ACCESSION   DAOQQY010000198 DAOQQY010000000
VERSION     DAOQQY010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6886_length_5359_cov_5.314857"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="PLE23_09355"
     CDS             complement(<1..985)
                     /locus_tag="PLE23_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMX83511.1"
                     /translation="MSEQWPKLSKQDWLTLVQKELKDKDYASIVQRISPNISIEPYYT
                     ADETSGRMPPLIVSNTWESAEDFHFTGHNAADTNAAILEALNGGLQSLSIHCDTVPDP
                     DALKLVLNGVSLGSVSVFFSFPQGTDALSVRTLLENVCHTGGISHDLLRGGILSNLHD
                     AEMIAGFPLIKPLPINTGDHQCTQTPEIVLARLLFEADSIWEKSNPSDHHALVRQFHF
                     TLHIGNDFLTELSKLRAMYLLWANYQLARGIERPSEPYIHAVFCRDAYTEDENKNMIK
                     ASAMAMAAVAGGAARITVTPTGTEQSSFQRRIARNVLNLLDLEAGMRLVPDP"
     gene            1223..2380
                     /locus_tag="PLE23_09360"
     CDS             1223..2380
                     /locus_tag="PLE23_09360"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HMX83512.1"
                     /translation="MHYYSLGRLPHKRHTQFRKENGELYHEELFSTEGFSDKYSLLYY
                     CHPPTQIIQVGHPYSVAPETIHDKQLKHRCLNGFQVRPDVDFLKSRKQVLVNSDCIIS
                     LAAPLNGSADYFFKNADADEVYFVHEGSGTLKTMYGNLKFGYGDYLVIPRGTTYQFEF
                     DTHDNRLLIVESFSAIDTPNRYRNEFGQLLEHSPFCERDIRKPSDLETHDIKGDFLVY
                     IKKQDMMYPYHYKFHPFGVVGWDGYVYPYAFSIHDFEPITGRVHQPPPVHQTFQARGF
                     VICSFVPRLYDYHPLAIPAPYNHSNIDSDEVLYYVDGDFMSRKHVEKGMITLHPGGIP
                     HGPHPGTVEKSIGKKETLELAVMIDTFKPLLMTKCAEKIESPDYYLSWVEE"
     gene            2414..3538
                     /gene="hppD"
                     /locus_tag="PLE23_09365"
     CDS             2414..3538
                     /gene="hppD"
                     /locus_tag="PLE23_09365"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HMX83513.1"
                     /translation="MDTLVQSKPNTSQDTFPILGTDYIEFYVGNAKQSAIYYQATFGY
                     KWVAYMGPETGVKDKVSYVLQQDKIRLVLTSSLFPDTEISRHVAEHGDGIKVLALWVD
                     DAEWSFNTAVERGATIAGELKIEEDEFGVLKTASIHTYGETIHTFVERKNYHGAFRPG
                     YEKRESSFPVDPVGLMYIDHCVGNVELGGMNKWVKFYQDVMGFNLLITFDDNDISTEY
                     TALMSKVVSNGNGYIKFPINEPAKGLKKSQIEEYLDFYRAAGVQHIAVATNNIIDTVT
                     KLRARGIDFLYVPDTYYDDVFERVGKIEENIEDLKRLNILIDRDDDGYLLQLFTKPVQ
                     DRPTVFYEIIQRKGATSFGKGNFKALFEAIEREQELRGTL"
     gene            3614..5071
                     /locus_tag="PLE23_09370"
     CDS             3614..5071
                     /locus_tag="PLE23_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83514.1"
                     /translation="MKLLLSVIVSILAVCSVYAQPGTYPESLKQSASCSMSAVYMMNE
                     SVHGLLIVQRIFENDNQTLNKYVDLESQKINFYGNEDLPKNVFDDPDHWFYAVSPIDW
                     YSNIQKRCGSSASSGLSATAGSKLRETRLLLDDINKMRFTIDDELRKRDLSKVDEINA
                     VYKTLEQAAQQCDQLLALRNQLQAALPANTSRLKPDQLELVNNLNAVYKASEQLILAV
                     RDDKEKIVKKQLSELQTAYKNFQMKRIAMTRTAHSTGANVELNFENIAKKTETLIQEA
                     KAYLGDSPFDSKYAAYGRNYFYYNVKMLSLFNKYGKGIVYEMNQLFLLSGISYHGFLE
                     LPHYVKIIYPEKRLEVERSVVKTETVEELPETLRSRRVVNKQRLIVADGPECVLEISD
                     NQEVDGDIISINYNGKWIIENYPLQKEAVKLPILLNTEGKNYLLLHAENQGTIPPNTI
                     LIRYKYEGRFKRVILNSTDKESEMIEIKPNIVKKG"
BASE COUNT         1581 a         1139 c         1261 g         1378 t
ORIGIN      
        1 ccgggtcggg tacgagacgc atgccggctt caaggtcgag caggttgaga acgttgcggg
       61 ctatacgtcg ctggaatgaa ctttgctctg tacctgtggg cgttacagtg atgcgggctg
      121 caccacctgc tacggctgcc attgccatag ccgaggcttt gatcatgttc ttgttctcgt
      181 cttcggtgta agcatctcta cagaatacag catgaatgta gggctctgaa ggacgttcaa
      241 taccccgggc tagctgataa ttagcccaca gcagatacat ggctcgcagt tttgacaatt
      301 cggtcaggaa atcgttacct atgtgtaagg taaaatggaa ttgccggaca agtgcatggt
      361 ggtcagaagg atttgatttt tcccagatgc tgtctgcttc gaacaaaagc ctggcgagga
      421 cgatttcggg tgtttgggtg cattggtgat caccggtatt tatcggcagg ggttttataa
      481 gtggaaatcc ggcaatcatt tcagcatcgt gtaggttgct gagtatgccg cctcggagca
      541 gatcgtgaga aatgccgccc gtatggcaaa cattttcgag aagcgtcctt accgacagag
      601 catcggtgcc ttgcgggaaa ctaaaaaata ccgaaactga gccaaggctg acaccattca
      661 atacaagctt taaagcatcc gggtcaggaa cggtgtcaca atggatggac agtgattgca
      721 gtccgccatt gagtgcctcg agtattgctg cgttggtatc agctgcattg tggccggtaa
      781 aatgaaaatc ttctgccgac tcccaggtat tgctgacaat gagtggtggc atacgaccgc
      841 tggtttcgtc ggctgtataa tacggttcga tgctgatgtt agggcttatg cgctgcacaa
      901 tagaggcata atccttgtct ttcagttcct tttgtactaa ggtgagccag tcttgtttgg
      961 agagcttagg ccattgttca ctcattgata atcattttac aggctgctaa attaagcttt
     1021 ttgacacaag acgccaaata ataaaatttt ctggcttgaa tttggctaaa aacatgtagt
     1081 gaagtggtaa ttatcatgaa tttggcaggt gttttttacc atatagaaag cttcatcagg
     1141 tggaaagtcg attttaacaa agcgaaataa agcataattc gcaaataagc attaattttg
     1201 gagcaccaac aatatttgca acatgcatta ttattcatta ggcaggctgc cacacaagcg
     1261 tcacacacag ttcaggaaag agaacggtga gttgtaccac gaagaactct tctctacaga
     1321 aggcttcagt gataaatatt cgcttctgta ctactgtcat ccacctacac agattatcca
     1381 ggtggggcat ccttattctg ttgctcctga gaccatacac gacaagcagc tcaagcatag
     1441 gtgtctgaat ggttttcagg tacggccgga tgtagatttc cttaagagta ggaagcaggt
     1501 gttggtcaat tcggattgta tcatcagcct ggcagcgcca cttaacggca gcgcagacta
     1561 ttttttcaaa aatgcagatg ccgacgaggt ttatttcgta cacgaaggtt ctggtacgct
     1621 taagactatg tacggcaacc tgaagttcgg ctatggcgat tatctggtaa taccacgtgg
     1681 tacaacctat cagttcgagt tcgacacaca cgataaccgc ctgcttatag tagaatcatt
     1741 ttctgccata gatacaccca accgttacag gaatgagttt ggccagttgt tggaacattc
     1801 gcctttctgt gagagagata tacgcaaacc atccgatctg gagacacatg acataaaagg
     1861 tgattttctg gtctatatca agaagcagga tatgatgtac ccataccatt ataagtttca
     1921 cccttttggg gtggtcggct gggacggtta tgtttatcct tatgcctttt ctatacatga
     1981 ctttgagccg ataaccggac gagtacatca gccaccaccg gtacatcaga cttttcaggc
     2041 acgcgggttt gtaatctgtt cgtttgtgcc gaggctgtac gattatcatc cgctggctat
     2101 accagcacct tataaccata gcaacattga cagtgatgaa gtgttgtact atgtagatgg
     2161 tgactttatg agccgcaagc atgtagagaa aggaatgata acactacacc cgggaggcat
     2221 accacacggt ccgcatccgg gtacggtaga gaaaagcatc ggcaagaaag agaccctcga
     2281 actggcagtg atgatagata ctttcaaacc cttgctgatg accaaatgtg cagaaaaaat
     2341 cgaatcaccc gattactacc tgagttgggt agaagaataa tcttttttaa ttaaaaaaaa
     2401 caaaataata ataatggata cactcgttca atcgaaaccc aacaccagcc aagatacatt
     2461 tccgatcctt ggcacagatt atatcgaatt ttatgtaggc aatgccaagc agtcggccat
     2521 atactaccaa gcaactttcg gatataaatg ggtcgcctac atggggcctg aaactggcgt
     2581 aaaagacaag gtgagttatg tattacagca agataagatt aggcttgttc ttacatccag
     2641 cctgtttccg gatactgaga taagccgcca tgtagcagaa cacggtgacg gtatcaaagt
     2701 attggcgctg tgggtggacg atgccgagtg gtctttcaat actgcagtgg agagaggagc
     2761 cactatagca ggagaactta agatcgaaga agacgaattc ggtgtactaa aaactgcatc
     2821 cattcatacc tatggcgaga ccatccacac attcgtagaa agaaaaaact atcatggtgc
     2881 ttttagaccg ggatatgaaa aacgcgaaag tagctttccg gttgatccgg ttggtctgat
     2941 gtacattgac cactgcgtag gcaatgtaga gttgggcggc atgaacaaat gggtcaagtt
     3001 ttatcaggat gtgatgggct tcaatctgct catcaccttt gatgacaacg atatttcaac
     3061 agaatacacc gccctgatga gcaaagtagt ctctaatggc aatgggtata tcaaattccc
     3121 gatcaacgaa cctgccaaag gacttaagaa atcgcagatc gaagaatatc tcgactttta
     3181 ccgagctgcc ggtgtacagc atatagcagt agctaccaac aacatcatcg atacagtcac
     3241 caagctgaga gccaggggca ttgacttcct gtatgtaccg gacacttact acgacgatgt
     3301 gtttgaaaga gtgggcaaaa tagaagagaa tatcgaagac ctgaagcgac tgaatatact
     3361 catagaccgc gatgatgacg ggtacctgct tcaattgttc accaagccgg ttcaggaccg
     3421 acctacggta ttctacgaaa ttatccagag aaaaggagct acttcattcg gtaaaggcaa
     3481 cttcaaggca ttgtttgaag cgattgaacg cgagcaggag ctgagaggta cactctgatc
     3541 ggaatttctt aataagccgt taaagcacaa caccgttgca accaatggca gcttattgac
     3601 gttagtgcag tgtatgaaac tgttactgag cgttattgtc agcatattgg ctgtgtgtag
     3661 tgtttatgca caacccggta cctatcccga atcacttaag cagtccgctt cgtgcagtat
     3721 gtcggctgtg tatatgatga acgagtcggt acacggtctg ctcattgtac aacgcatctt
     3781 cgaaaacgac aaccagaccc tgaacaaata cgtcgatctg gagagtcaga agattaattt
     3841 ttatggcaat gaagatttgc ccaaaaacgt atttgacgac cccgaccact ggttttatgc
     3901 cgtgtcgccc attgattggt atagtaatat ccagaaaaga tgtggatcgt ctgcctcatc
     3961 cggattgtca gcaacggcag gtagtaagct ccgtgagaca agactgctgt tggatgatat
     4021 caacaaaatg cggtttacca tagacgacga actccgaaag cgcgacctgt ctaaggtgga
     4081 cgagataaac gctgtgtata agacactgga acaagcagct caacaatgtg atcagttgct
     4141 cgctctcaga aatcaacttc aggcagcact gccagccaat accagccggt taaaaccgga
     4201 tcaactggag ttagtcaata atctgaacgc agtttataaa gcctcagaac aattgattct
     4261 tgccgttcgg gatgataaag agaaaatagt caaaaagcaa ctgtcagaac tgcagactgc
     4321 ttataagaac ttccagatga aacggatcgc catgacgcgg acagcacata gcaccggtgc
     4381 aaatgtggaa ttgaattttg aaaatattgc caaaaaaaca gaaacgctga tacaggaagc
     4441 aaaggcatat ttgggcgatt cgccatttga ttcaaaatac gctgcttatg gaagaaatta
     4501 cttctactac aatgtaaaga tgttgtcact tttcaataaa tacggcaaag gaattgtcta
     4561 tgaaatgaac cagctcttcc tgttatcagg catttcatat catggctttc ttgaactgcc
     4621 acattatgtc aagatcattt atccggagaa gcgcctcgaa gttgaaaggt cagtcgtcaa
     4681 aacagagaca gtcgaagaac tgccagagac gctccgaagc aggcgcgttg taaacaagca
     4741 gaggctgatc gtggcagacg gaccggagtg tgttctcgag atcagcgaca accaggaagt
     4801 ggacggcgac atcatatcca tcaactacaa tggcaagtgg atcatcgaaa actatccctt
     4861 gcagaaagag gctgtgaagc tgcccatttt gttgaataca gaagggaaaa actacctact
     4921 cctgcacgcc gaaaaccagg gtaccatccc gcctaacacc atcctcatcc gttataagta
     4981 cgaagggaga ttcaaacgag tcatactcaa ttctaccgac aaagaaagtg agatgataga
     5041 aatcaagccg aatattgtga agaaagggta gaatgcaggc acacaactaa tatttttcca
     5101 cattttcgta tagttaagtg aaacaagtgg ggcgctttgt gatagaatat ttatggagcc
     5161 tgtggctttt tgctctcttt ccatgaagct taccgaaaaa aatgcccgtt catccttcag
     5221 gataagaggc aaaaacgggc atttttatca gctaagcctc atttttccat aaatccactt
     5281 cgaggaaaat ttgaattctg ctccatattg attttctaag cttgcggata ttagcttttg
     5341 ttgtttgggc gtatccctg
//
LOCUS       DAOQQY010000199         5355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6899_length_5355_cov_4.716792, whole genome shotgun sequence.
ACCESSION   DAOQQY010000199 DAOQQY010000000
VERSION     DAOQQY010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6899_length_5355_cov_4.716792"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1078
                     /gene="murB"
                     /locus_tag="PLE23_09375"
     CDS             68..1078
                     /gene="murB"
                     /locus_tag="PLE23_09375"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMX83515.1"
                     /translation="MQVKHQFSLKELNTFGIGVFATAYCAAETEADVLEAIGQTSLPV
                     FVLGGGSNILFTEDIQAFVLHNKITGIEIVEESDSDIIIKAGGGEVWHSFVLWCIDKD
                     YGGIENLSLIPGTVGAAPVQNIGAYGVELKDVFHSLEAIDLLTGNTLTFSKEACKFGY
                     RDSIFKHEWKGRLIISRVYFQLTKKNHKLHSSYAALKSELDKQHITDPGILDISRAVI
                     AVRQSKLPDPKTLGNAGSFFKNPEISRELFLKIQEKYPSIPHYPAAEGLVKIPAGWLI
                     EQRGWKGRRKGNCGSYEQQALVLVNYNDATGKEILQLATEIIEDVYDHFGVKLSPEVN
                     IM"
     gene            1092..2996
                     /locus_tag="PLE23_09380"
     CDS             1092..2996
                     /locus_tag="PLE23_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HMX83516.1"
                     /translation="MNNSLEILKKYWHYDSFRPLQQEIVDCILAGKDAIALLPTGGGK
                     SVCFQVPALMMEGVCIVVSPLIALMKDQVDQLKRRNIKAEAVYSGMHFRDIERIMDNV
                     ILGDTKLLYISPERLVSVKAMDKIMQMKVSFIAVDEAHCVSQWGHDFRPTYLHIKSLR
                     ELFADVPILAVTATATRDVVKDIQIQLALRLPQIFRSGFSRENLSYSVIREEGKLHRL
                     LQLLNNVPGTALIYVRNRKLTKETAIFLQQKGISADHYHAGLEAEERFRKQEDWIKNK
                     IRVMVCTNAFGMGIDKPDVRLVVHLDVPDTLEAYFQEAGRAGRDGKKSWAIILYNESD
                     VKALERSFELSFPEIEVIKRIYQALGSYLQLATGAGAGVSFDFDLLVFSKNFGFEPAT
                     VSSTLKILERSGVLVVTESVFNPPRLVFNTDRNELYQFQLKNPLYEKLITTILRLHQG
                     VFRQPVVLDEHRLCFMMNIKVVQLHEMLNYLKMIGFIEYFSRKDTPVITFLTDRVEAK
                     YLSIDLQKYTFLKERAAAKLHSVLKYLQRPVCRDVQLLEYFDEKPVGECGICDICVMR
                     KRKTESERVEAELLGTIKRKLQENRMNVDELIASFSIKYKSKVLEILQYLVNEKQVRI
                     KDDEMIEFIG"
     gene            2993..4087
                     /locus_tag="PLE23_09385"
     CDS             2993..4087
                     /locus_tag="PLE23_09385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX83517.1"
                     /translation="MKKIIFCVTNDLVTDQRMMRICSTLAEDGYEVELVGRLRKNSTT
                     VVAKSSKQTRLKCWFDAGKLFYVEYNIRLFFYLIFRRFDILGAVDLDTILPVYLVSKL
                     RFKPATFDAHEYFEEVPEVSDRAFVKKIWTIVGNWCVPGMKLCYTVGPALADIFTKKY
                     KRPFHVIRNMPIQKDIASVQLPLIAQPYLLYQGALNEGRGLEPMIEAMNNLPEYRLII
                     AGAGYLKEELILAVKKYGLQKRVIFTGNLDPDQLDGFTAGAYVGLNLLENKGKSYYYS
                     LANKFFDYVQFGIPVLIMDFPEYAALNKEYEVAVLIDNLRPETIIRQLQLLHSDLSLH
                     TRLRMNCLKAKKQWIWEGEATRLKELYHMI"
     gene            4195..4292
                     /gene="ffs"
                     /locus_tag="PLE23_09390"
     ncRNA           4195..4292
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLE23_09390"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4320..4976
                     /gene="fsa"
                     /locus_tag="PLE23_09395"
     CDS             4320..4976
                     /gene="fsa"
                     /locus_tag="PLE23_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HMX83518.1"
                     /translation="MKFFIDTANLDQILEAKSFGILDGVTTNPSLMAKEGIKGADNIM
                     AHYRKICEIVDGDVSAEVIATEYKAMIKEGVALSEIAPNIVVKVPMTVDGVKAIRYFT
                     ENGIRTNCTLVFSAGQAILAAKAGATYVSPFIGRIDDMNWDGMMLIQQIAEIYAIQQY
                     DTEILAASIRSSKQIVEAAESGADVVTCPLDSIKGLFYHPLTDIGLAKFLADHKKVNG
                     "
     gene            complement(4986..>5355)
                     /locus_tag="PLE23_09400"
     CDS             complement(4986..>5355)
                     /locus_tag="PLE23_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83519.1"
                     /translation="LKMGHMVNKYDPRLFSIFRPSVQPYMISWIKYDPAREISNLKLP
                     ALIIQGTKDLQVGIVDAKLLSQAKPNARYVLIQNMNHVLKDITSDDRNDNYKSYNDPV
                     LPLDKTLVKEIQKFIKKYGK"
BASE COUNT         1665 a          970 c         1229 g         1491 t
ORIGIN      
        1 gtttatttac ctgttagcaa aggattctgg ggattgaaaa tagtggatgt taaaggattg
       61 cgtgccaatg caggttaaac atcaattttc acttaaggag ctgaatactt ttggtatcgg
      121 ggtttttgca accgcttatt gcgcagctga aacggaggca gatgtactgg aggctattgg
      181 gcagacttcg ttacctgtgt ttgtgctggg tggtggaagc aatatcttat ttaccgagga
      241 cattcaagcc tttgtattac acaacaaaat aacaggtata gaaattgtag aagaatctga
      301 ttcagacatt atcataaagg caggtggcgg agaggtttgg cattcgtttg ttttatggtg
      361 tatagataaa gattacggag gcattgaaaa tctctctctc attcccggta cagtaggtgc
      421 cgctcctgtg cagaatattg gtgcgtatgg tgtagagctc aaagatgtgt ttcattcact
      481 agaggcaatc gatttgctca caggtaatac actcactttt tcaaaggaag cgtgcaaatt
      541 cggttatcgc gacagcattt ttaaacacga atggaaaggc agacttatca tttcaagagt
      601 gtattttcaa ttgactaaaa agaatcacaa actgcactca tcttatgcag ctctcaagtc
      661 agaactcgat aaacagcata ttactgatcc gggtatattg gatatcagca gagcggtgat
      721 agcagtaagg caaagtaaat tacctgatcc aaaaacgctt ggtaatgcag gttcgttttt
      781 caaaaatccg gaaatatctc gtgagttatt tttaaaaata caggaaaagt atccttctat
      841 cccacactat ccggcagctg aaggcttggt gaaaatacct gccggttggc taatagaaca
      901 acgtggatgg aaaggcaggc gaaaaggcaa ctgcggtagt tatgaacaac aagcacttgt
      961 gttggttaat tataatgatg ctaccggtaa ggaaatattg caactcgcca ccgagataat
     1021 cgaggacgtg tacgatcatt ttggtgtgaa gctcagtccg gaagtaaaca ttatgtaggg
     1081 caatcaacgg catgaacaat tcgctcgaaa tccttaaaaa atactggcat tacgattctt
     1141 tcaggccttt gcagcaagaa atagtggact gtatcttagc cggtaaagat gctattgcac
     1201 tgctacctac cggtggtgga aaatcggtct gctttcaggt gcccgccttg atgatggaag
     1261 gcgtgtgtat agtagtgtcg cctctgatag cattgatgaa agaccaggta gatcaactga
     1321 agcgtcgaaa tatcaaggca gaagctgttt attcaggtat gcacttcagg gatatagaaa
     1381 gaataatgga caacgtaata ctgggtgata caaaactctt gtacatttct cctgaacgcc
     1441 tcgtatctgt aaaggcaatg gacaaaatca tgcagatgaa ggtaagtttc atagcagtag
     1501 atgaggcgca ctgtgtatcg cagtgggggc atgatttcag gcctacctat ctgcatatca
     1561 agagtttaag agaattattt gcagatgtgc ctatacttgc cgttacagca actgctacac
     1621 gcgatgtagt aaaagacata cagatacagt tagcattaag attgccacag atatttagat
     1681 cgggtttttc aagagagaat ttgtcatatt cggtcatacg ggaggaaggc aaactacacc
     1741 gattactcca attgttgaat aatgtaccgg gtacagcact tatatatgtg cgcaacagaa
     1801 agcttaccaa agaaacagct attttcttac agcaaaaagg tatttcagcc gatcattatc
     1861 atgcaggcct tgaagctgaa gaaaggtttc gcaaacaaga agactggatt aaaaacaaga
     1921 taagggtcat ggtctgtacc aatgcctttg gtatgggcat cgacaagccc gatgtaaggc
     1981 ttgtggtcca tctggatgtg cccgatacac ttgaggcata ttttcaggaa gcaggtaggg
     2041 cagggcgtga tggtaaaaag tcatgggcaa tcattttata caatgaaagc gatgtaaaag
     2101 cacttgaaag aagcttcgaa ctgtcatttc cggagattga agtgatcaag agaatatatc
     2161 aggcattggg tagttaccta cagttggcaa caggtgccgg agcaggcgtt tcgtttgatt
     2221 ttgatctatt ggtttttagt aaaaattttg gtttcgaacc tgccacagta tcatcaacct
     2281 taaaaatcct cgaacgttca ggagtgctgg ttgtgacaga gtcagtattt aacccaccca
     2341 gactggtatt caataccgat cgcaacgaat tgtatcagtt tcagctaaag aatccattgt
     2401 atgaaaagct gataaccact attctaagat tgcaccaagg tgtgttcaga cagccggtag
     2461 tacttgatga acacaggttg tgttttatga tgaatataaa ggtagttcaa ctacacgaaa
     2521 tgctcaatta tctgaaaatg attgggttta tagaatattt ttcgcgaaaa gacacaccgg
     2581 tcatcacctt cctcaccgat agggtggaag caaagtatct aagcatagac ctgcagaaat
     2641 acacattctt gaaagagcgg gcagctgcca agttacatag tgtactcaaa tatttgcaac
     2701 gaccggtatg tcgtgatgta cagcttcttg aatattttga tgaaaaaccg gtcggagaat
     2761 gtggtatctg tgatatttgt gtaatgcgaa aacgcaagac agaaagcgaa agagtcgaag
     2821 cagaattgct tggtaccatc aagcgtaaac tccaggagaa tagaatgaat gttgatgagc
     2881 taatcgcttc attttcaata aaatataaat caaaagtgct tgaaatatta cagtatctag
     2941 tcaatgaaaa gcaagtcagg attaaggatg acgaaatgat agaattcatc ggatgaaaaa
     3001 gataatattt tgtgtgacca acgacctagt caccgaccaa cggatgatgc gcatctgcag
     3061 tacacttgcc gaagacgggt acgaggtgga gcttgtaggc cgccttcgga aaaattcgac
     3121 tactgtagta gcaaaatcat ccaagcagac caggctgaaa tgctggttcg atgcaggcaa
     3181 attattttac gtagaatata acatccgttt attcttttat ttgattttca gacgttttga
     3241 tatacttgga gcagttgatc tggacacaat attgccggtg tatttggtat cgaagctacg
     3301 gttcaagccg gctacatttg atgcacatga gtatttcgaa gaagttccgg aggtcagcga
     3361 cagagctttt gttaagaaga tatggacaat agtaggtaac tggtgtgtac cagggatgaa
     3421 attatgctac acagtggggc cggcattggc tgacattttt accaaaaaat acaaacgacc
     3481 attccacgtt ataaggaata tgcctattca aaaagatatt gcatcagtac agttgccgct
     3541 tattgcacag ccatatttgc tttatcaggg agctttgaat gaaggtcgcg gactcgagcc
     3601 aatgatagaa gccatgaaca atctgcccga atacagactc atcatagccg gagccggata
     3661 ccttaaagaa gaactgattt tggcagttaa gaagtatggt ttacagaagc gcgtaatttt
     3721 tacaggcaat ctggaccccg atcagcttga tgggttcaca gcaggagctt atgttgggtt
     3781 aaatttgctt gaaaacaaag gaaaaagcta ctattactcg cttgcaaaca agtttttcga
     3841 ctatgtacag tttggtatcc cggtgttgat tatggatttt ccggaatacg ctgcgctcaa
     3901 taaggagtat gaagtggctg tgctgataga taatcttaga ccggaaacaa tcatcaggca
     3961 actacagctt cttcattctg atctgtcgtt acacacaagg ttaaggatga attgcctgaa
     4021 ggcaaaaaaa caatggatat gggaaggaga agcaacaaga ttgaaagaac tataccacat
     4081 gatttagaag taataaacag acaatcagct gtttgtgttt tgtgaaattc aaatatcacg
     4141 aaaaagaatt caaatatata tttatttaat gaaaaaaata attacctttg cacccggtga
     4201 gggtcatgtg accagctcct gccgaacccc cccagggcag aaatgcagca agggtaggtg
     4261 gttgagcggt acgacgtgat tcctcacctt cttagtttct atactcaaaa catcttctta
     4321 tgaaattttt tattgacaca gccaacctag atcagatact agaagccaaa tcatttggta
     4381 ttcttgatgg tgtaactacc aatccgtctc tgatggcaaa agaaggaata aagggtgccg
     4441 acaacatcat ggcacattac cgcaaaatct gtgaaatagt agatggagat gtaagtgcag
     4501 aagtgatagc tacagaatat aaagcaatga tcaaagaagg tgtggcattg tccgagatag
     4561 cacctaatat tgtagtaaaa gtaccaatga ctgtggatgg agtgaaggcc atccgttact
     4621 ttacggaaaa cggtatccgt acaaactgta ccttggtgtt ttcggctggt caggctatcc
     4681 ttgcagccaa agcaggtgcc acctatgttt cgcctttcat tggtcgtatt gatgatatga
     4741 actgggacgg tatgatgctt atccaacaga tagcagaaat atatgccatc cagcaatatg
     4801 ataccgaaat actggcagct tctatccgtt cgtcgaaaca gattgtcgaa gctgcagaaa
     4861 gtggtgctga tgtagtcact tgtccgctag attctataaa aggattattc tatcatccgt
     4921 tgacagacat cgggttagcc aaatttctgg cagaccacaa gaaagtgaat ggatagtaac
     4981 ggaacttatt ttccgtattt tttaatgaat ttttgaattt ccttaaccag tgttttgtct
     5041 aaaggtaaaa cagggtcatt ataggattta taattatcgt ttctgtcatc gcttgtaatg
     5101 tcttttagta catggttcat attctgaatc aatacataac gtgcatttgg tttggcctga
     5161 gacagtagtt tggcatccac aatccccacc tggaggtctt ttgtaccctg tatgataagt
     5221 gcaggaagtt tcaagttgct tatttctcgg gccggatcgt attttatcca cgaaatcata
     5281 taaggctgca ctgatgggcg gaatatagaa aacagcctcg ggtcatattt atttaccatg
     5341 tgtcccattt tgagg
//
LOCUS       DAOQQY010000200         5345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6913_length_5345_cov_5.453875, whole genome shotgun sequence.
ACCESSION   DAOQQY010000200 DAOQQY010000000
VERSION     DAOQQY010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6913_length_5345_cov_5.453875"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLE23_09405"
     CDS             complement(<1..238)
                     /locus_tag="PLE23_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMX83520.1"
                     /translation="MMVSRKYDCINCKTRCFSILDACDLETLKVISTYKHSRSINKGE
                     RLFTEGENVEGVYFIKKGFLKIEKILAGDFLKILA"
     gene            complement(354..1220)
                     /locus_tag="PLE23_09410"
     CDS             complement(354..1220)
                     /locus_tag="PLE23_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83521.1"
                     /translation="MKLKNNLFIGLIFIQISNLFAQTHDWWANNVGWDQKTHYKYYID
                     IKPARMGPNAFYYASGFTGILDTQYTLEVKANSYFTKGEITLNPYLSVNIPFGKFASL
                     QVSGVPVEYFNTSHELKTERKIFWIGYYDKWAAGDLNVLFTGKIKSKPAMAIQVGLKT
                     ATGGRLDLARFTDSPQYFFNYAISFGNKNTMVHAVSGFTAWQTFDGDHPQDDAYIFDF
                     SLDQHLGKAWYLKPELYGFIAYLKHGDRPIIGKVSLFRKMEHGRFMIEFVKGIVDFPY
                     YGINLGISRSWR"
     gene            complement(1217..1741)
                     /locus_tag="PLE23_09415"
     CDS             complement(1217..1741)
                     /locus_tag="PLE23_09415"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMX83522.1"
                     /translation="MAKLTYPKTGDFPFYYEDYIKLVTEEDLMTAIKNDTAMVLDFLS
                     SNKQADWDFRYAEGKWTPREILLHIIDTERIMSYRALRASRGDRTHLPGFDQTGYIEN
                     SQAAKRSLSSLVAEFKSVRNASISLFKNMTKKEAERVGYANAQQITAKSIGFVIVGHA
                     RHHIQVLREKYSFA"
     gene            complement(1749..2423)
                     /gene="trmD"
                     /locus_tag="PLE23_09420"
     CDS             complement(1749..2423)
                     /gene="trmD"
                     /locus_tag="PLE23_09420"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMX83523.1"
                     /translation="MRFDIITVLPELLHGPLHHSIMQRAVDKGLVSFHIHNLRDYGLG
                     RTKQLDDYQYGGGAGMVMMCEPLAACIESLTNVRTYDHIIYVTPDGEQLTQGLVNRLS
                     LQGNLIIICGHYKGIDERIREKYVTLEVSIGDYVLSGGELAAAVLVDSVTRVIPGVLN
                     DETSALFDSFQDGLLAPPVYTRPADFNGMKVPDILLSGNDKLIDEWRTEQSYERTRKR
                     RPDLLK"
     gene            complement(2432..3019)
                     /locus_tag="PLE23_09425"
     CDS             complement(2432..3019)
                     /locus_tag="PLE23_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMX83524.1"
                     /translation="MDSVSYSLGVLVGQNLKNQGFKNLDASSITAGLKDAIEGKQLAI
                     SSDQAGKIVNDYLQKAAQAEAGPAIEEGKKFLAENGKKPGVVTLPSGLQYMVIKEGTG
                     PKPKLSDKVTTHYVGTLINGEEFDSSVRRGEPATFPVSGVIKGWTEALQLMPVGSKWK
                     LFVPYDLAYGERGAGGQIKPYSTLIFEVELLEIAK"
     gene            complement(3134..4522)
                     /locus_tag="PLE23_09430"
     CDS             complement(3134..4522)
                     /locus_tag="PLE23_09430"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HMX83525.1"
                     /translation="MDVTQDYILKNKQRFLDELFELLRIPSVSADSKHKDDMVATANF
                     ICNSFLKAGADKAQVMQTDGHPVVYAEKILDPSLPTILVYGHYDVQPPDPLELWTSPP
                     FEPVIKTTDIHPEGAIFARGSCDDKGQVWMHVKAFEAMVSSSELPCNVKFMIEGEEEV
                     GSPNLGPFCRQHKDLLKADVILVSDTGIIANDTPSIDAGLRGLSYVEVEVTGANRDLH
                     SGIYGGAVANPINVLCEMIASLKDANKHITIPGFYDDLLELSSEERAALGKTPFDLEA
                     YKQDLDINDIEGEAGYTTIERTGIRPTLDVNGIWGGYIGEGAKTVLPSKAYAKISMRL
                     VPHQRPEAITALFQKYFEAIAPASVTVKVKPHHGGKPVVVPTNSLEYKAAEMAMKKTF
                     GKDPIPTRSGGSIPIIALFEEVLGIKSVLMGFGLDSDALHSPNEHFGLFNFYKGIETI
                     PYYYQYYSQLKK"
     gene            4697..>5345
                     /locus_tag="PLE23_09435"
     CDS             4697..>5345
                     /locus_tag="PLE23_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83526.1"
                     /translation="MKWKVRIFFSHFAPILLATIVAIIVFLPDVHAQQIEQNQKGDWI
                     LINKDGSTRILTPANKSDLKLLNTEPAYVRSSFLTELADKITQDLKESENKIQLNNIE
                     SERLRINRDLLEQTLKTEKEELGEAEINQTRKQINELKIIISYLKKENLQYQNDIENL
                     KSLLKISGPKQIEAYRTFCAERDKKHKPPPILNNPNIKRQVLPNAGKSIDNIPPDS"
BASE COUNT         1497 a         1150 c         1088 g         1610 t
ORIGIN      
        1 cagctagtat tttgagaaag tctcctgcca gtattttttc aatttttaga aagccttttt
       61 tgataaaata aaccccttcc acattttcac cttcagtaaa cagacgttca cctttgttga
      121 tacttctcga atgtttataa gtactgataa ccttcagggt ttccaaatcg catgcatcaa
      181 gtatcgagaa acatctcgtc ttacagttga tacaatcgta ctttctggac accatcatag
      241 gcacaccaat atttcaaaga ggaaattcct ctcgagtaaa tcaatggtaa aattaagttt
      301 taggatcggt aaacacaaat tttatctgca tttagatgcg acaaccgtaa tatttaacgc
      361 cagcttcttg aaatgccaag gtttatgccg taatatggaa aatcaactat tccttttaca
      421 aattcaatca taaacctacc atgctccatt tttctgaaca gagatacctt gcctataatt
      481 gggcggtcgc catgcttcag ataggcaatg aatccgtaga gctctggctt tagataccaa
      541 gcttttccaa gatgttggtc gagtgaaaag tcaaatatat aggcatcatc ctgcgggtga
      601 tcaccgtcaa atgtctgcca ggccgtgaat ccgcttactg catgtaccat ggtgtttttg
      661 ttgccaaatg aaatagcata attgaagaaa tactgtggcg aatcggtaaa gcgtgcgaga
      721 tcgaggcggc caccggtagc ggttttgagg cctacctgta tagccatagc tggttttgac
      781 tttatttttc cggtaaacag aacattcaga tcgccggctg cccatttgtc gtaatatccg
      841 atccagaaaa tctttctttc ggtcttcagt tcatgtgatg tgttgaaata ttcgaccggt
      901 acaccggata cttgtagcga tgcaaatttc ccgaagggta tattcactga caaataagga
      961 ttgagcgtta tttctccttt tgtgaaataa gaattggcct ttacttccaa ggtatattgt
     1021 gtatcaagaa tgcccgtaaa tccacttgcg taatagaagg cattcgggcc catccgagcc
     1081 ggtttgatat cgatgtaata tttgtaatgc gttttttggt cccagcctac attattggcc
     1141 caccaatcgt gcgtctgtgc gaacaagttg cttatctgaa tgaaaattaa gccaataaaa
     1201 agattatttt ttagtttcat gcgaaactgt atttctccct taatacctga atatgatgcc
     1261 tggcatgacc aacgatgaca aagcctattg acttggccgt tatttgttgg gcattggcat
     1321 atccgaccct ttcggcttct tttttggtca tattcttaaa aaggcttatg cttgcgttgc
     1381 gtaccgattt gaattctgct acgagtgatg acaaagatct cttggcagcc tgcgaatttt
     1441 cgatatatcc tgtttggtca aagcctggaa ggtgcgtgcg gtcgcctctt gatgcccgga
     1501 gtgccctata tgacataata cgttctgtat caatgatatg tagtaatatt tcacgaggag
     1561 tccatttgcc ttctgcatag cggaaatccc agtcggcttg cttattggaa ctcagaaagt
     1621 caagtaccat tgcagtatca tttttgatgg ctgtcatcag gtcttcctct gtcaccagct
     1681 tgatataatc ttcatagtaa aaaggaaaat cgccagtctt tggataggta agcttagcca
     1741 tattattttt attttaacag atctggtcgg cgctttctgg tacgttcgta cgactgttca
     1801 gtgcgccatt catcaataag tttgtcattg cccgaaagta gaatgtccgg tactttcatc
     1861 ccgttgaaat ctgccgggcg ggtatataca ggcggagcga gcaatccgtc ctgaaacgaa
     1921 tcaaagaggg cagaagtctc atcgttgagt acgcccggta tcactctggt aacactgtcc
     1981 acgagtactg cagctgccag ctcgccaccc gacagaacgt agtcgccaat ggacacttca
     2041 agcgttacat acttttcgcg tatgcgttcg tcaattcctt tgtagtgtcc acagatgatt
     2101 atcaggtttc cttgcagtga tagtcggtta acaagcccct gtgtgagctg ttcgccatcc
     2161 ggcgtcacgt aaatgatatg gtcgtatgta cgtacattag tcaatgactc gatgcaggct
     2221 gcgagtggtt cgcacatcat taccattccg gcaccgccgc catactgata atcatccagt
     2281 tgtttggtac gacccaagcc ataatcgcgt aggttatgta tgtggaacga caccagcccc
     2341 ttatcaacag ccctttgcat gatagagtga tgcaaagggc cgtgaagtaa ttcgggtaat
     2401 accgtgatga tatcgaatct caccgggtta attattttgc tatttcaagg agttctactt
     2461 caaagatgag tgttgagtat ggtttaatct ggccacctgc acctctttcg ccgtaagcaa
     2521 gatcataagg tacaaacagt ttccactttg agccaacagg catcagttgc aatgcttctg
     2581 tccagccttt gataacgcca ctgaccggga aggtggcagg ctcacctctt ctaactgagc
     2641 tgtcaaattc ttcaccattg atgagtgtac ctacataatg tgtagtaact ttgtcgctaa
     2701 gctttggctt tggcccggtt ccttccttga tgaccatgta ctgcaagccg cttggcaatg
     2761 ttacaacacc tggttttttt ccgttttctg ccaaaaattt ctttccttct tcaatagccg
     2821 gacctgcctc tgcttgtgca gctttctgaa ggtaatcatt tacgattttt cctgcctgat
     2881 cagatgaaat ggcaagttgt ttgccctcaa tggcatcctt aagcccagcc gtgatagacg
     2941 aagcatccag atttttgaaa ccctgatttt ttaaattttg cccgaccaag acgccgagac
     3001 tgtaacttac tgaatccatt ttaagatttg atttttgtgc aaatgccatc attgacacac
     3061 tgcataacat gattaataaa gagatttttt tcatttgtcg aaagttgtga tttgatttaa
     3121 aaacgcttgt gaattatttt ttcaattgcg aatagtattg gtaataatat ggtatggtct
     3181 caatgccttt ataaaaattg aataagccga agtgctcatt tggagaatgt agtgcatcag
     3241 aatcgaggcc gaagcccatc aagacacttt tgataccaag tacttcttca aataaagcta
     3301 ttataggtat gcttccgcca cttctggtag gtatcgggtc tttgccaaag gtctttttca
     3361 tagccatttc tgccgccttg tattcaagag agtttgtagg tactaccaca ggtttacctc
     3421 catggtgagg ttttactttt acagtaacag aagcaggagc aatggcttca aaatatttct
     3481 gaaacaaagc agtgatggct tccggccttt gatgtggcac aaggcgcata cttatcttgg
     3541 cataagcctt tgatggcaat actgtcttgg ctccttcacc gatatagcct ccccagatac
     3601 cattgacatc aagtgtgggt cttatgcctg tgcgttcgat ggtggtgtag ccggcttcgc
     3661 cttcaatatc attaatatct aaatcttgct tgtatgcttc aaggtcaaaa ggggttttgc
     3721 caagtgctgc tcgttcttca gaactgagtt cgagtaaatc atcatagaag cccggaatgg
     3781 taatgtgttt attagcatct tttaacgatg ctatcatttc gcacaatacg ttgattgggt
     3841 tggctactgc tcctccgtaa atgccgctgt gcaggtcgcg gttggcacct gttacttcta
     3901 cctcaacata agacagccct ctcaggccgg catcaatgga aggtgtgtcg ttggcaatga
     3961 tacctgtatc ggaaaccaaa atcacgtcag ccttgagcag atctttgtgt tgcctgcaga
     4021 atggaccaag attaggcgag cctacttctt cttctccttc aatcataaac ttgacgttgc
     4081 agggcaattc gctactgctg accattgctt cgaatgcctt aacgtgcatc caaacctgac
     4141 ctttgtcatc acatgagcct cttgcaaaaa tggcaccttc cgggtgtata tcggttgtct
     4201 ttataacagg ttcaaaaggt ggcgatgtcc acagctcgag cggatctggt ggctgtacgt
     4261 cataatgtcc gtacacaagg atggtaggta gagaaggatc aagtattttc tctgcatata
     4321 ccacaggatg tccgtctgtc tgcattacct gtgccttgtc agcacctgct ttgagaaatg
     4381 agttgcagat aaaattagct gtcgctacca tatcatcctt atgcttcgaa tcagcactaa
     4441 ctgaaggaat tcggagcaat tcgaacaatt catccagaaa gcgttgttta ttttttaaaa
     4501 tgtagtcttg tgtaacgtcc atttggcaaa caaaatttaa ggtacaaaga tagaaatttt
     4561 ttattcctta acggataaaa ttggaattga gtatctgtac tgtgttatag ctttcttaaa
     4621 gtacctacac attattaacc ttcacaggta cgcacaattt gatttcaaat taagaagtaa
     4681 agtgtaaatt tgcctgatga aatggaaggt tcgaatattt ttttcacatt ttgctccaat
     4741 tttacttgcg accattgttg ctataatagt atttctcccg gatgtgcatg ctcaacaaat
     4801 agaacaaaac caaaagggcg actggatatt aatcaataaa gatggctcaa ctcgcatctt
     4861 aacccctgcg aacaagtcag atttaaaact gctgaacacc gaaccagcct atgtcaggag
     4921 tagttttctg acggaattgg cagataaaat cacccaagac cttaaggaat cagaaaataa
     4981 aatccagctc aacaatattg aatcagaaag attaagaata aaccgcgatc tgcttgaaca
     5041 aacactcaaa accgaaaaag aggaacttgg agaagccgaa ataaaccaaa cccgtaagca
     5101 aatcaatgaa cttaaaatta taatttctta cctaaaaaaa gagaatttgc aatatcaaaa
     5161 tgatatagaa aacttgaagt cattgttgaa aatttcgggt ccgaaacaga ttgaagcata
     5221 caggactttt tgtgccgagc gcgacaaaaa acacaaacca ccacctatat tgaacaaccc
     5281 gaacataaaa agacaggtat taccaaatgc cggtaaatct attgacaaca taccacccga
     5341 ttcaa
//
LOCUS       DAOQQY010000201         5340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6921_length_5340_cov_5.958373, whole genome shotgun sequence.
ACCESSION   DAOQQY010000201 DAOQQY010000000
VERSION     DAOQQY010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6921_length_5340_cov_5.958373"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..620
                     /locus_tag="PLE23_09440"
     CDS             87..620
                     /locus_tag="PLE23_09440"
                     /inference="COORDINATES: protein motif:HMM:NF016153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HMX83527.1"
                     /translation="MKNIVAKSWCCRYCQVSSWLHDKKQEVMDMKHVIIVSNLVITVL
                     APLVAVYAVGLARQGQHTRHLRVQKVLFGVCMSMLVIFELLIRASGGSGSLVEHSTHY
                     GQWYFKLILVSHIVGAVLTYIIWAVMVVLASRKWKRGQLPGKFSVTHKRLGYVTMIGL
                     WYTAVTAVIVSVLAFFG"
     gene            734..1639
                     /locus_tag="PLE23_09445"
     CDS             734..1639
                     /locus_tag="PLE23_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83528.1"
                     /translation="MKNILKVFVLFVCMYSLPGEILAQGCSDAGLCTLNSFKPKEQSD
                     FGSQFKLGASLGNADMAIQTIGAYAEFDKQIGAKFGFDVKLTALSQNGNQISNFGLSD
                     LYVNTNYRIGEKSRLALGVKIPLSDGNVKENEKSLPMDYQPSLGTYDLILGATTSLIG
                     IQLAAAVQFPLTQNKNEYFSAQYPIGTSLSAFQTTNKFKRSGDIMLRVSYPINLGRKL
                     SITPSILPIYHLANDKYTDISGAERVIAGSHGLTLNGNLYFDYLLGDKSAVQLNVGAP
                     FVVRDTRPDGLTRSFIATLEYRFTF"
     gene            2040..3017
                     /locus_tag="PLE23_09450"
     CDS             2040..3017
                     /locus_tag="PLE23_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HMX83529.1"
                     /translation="MHELWTKILSYTTLRDREKRRKYKYGYSVYVLAKAQREFRILLK
                     RYVKDSILITAGIFSAAFGLKGFLLTNHFIDGGATGISLLLSIVLKMPLPLFIILVNI
                     PFIYLAYKTVGKIFAIKTSLAITGLSIVLATVTFPNVTDDNLLVAIFGGFFLGAGIGL
                     AVRGGSVIDGTEVLAIYLSRKLSTTLGDIIILINIIIFSAAAYFLSIEAAMYSMITYL
                     SASKTLDFIAEGIEEYIGVTIISKQFEKIRQMIAERMGRGLTVYNGKRGYGKSGDTLE
                     IDIIFTVVTRLELSKLNVELEKIDPDAFVIMSAIKDTKGGMIKQRPLKH"
     gene            3327..4046
                     /locus_tag="PLE23_09455"
     CDS             3327..4046
                     /locus_tag="PLE23_09455"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HMX83530.1"
                     /translation="MCGRSSLTKNEKELEQRFGATFYSDELLRYNPLPNYNVAPTHYH
                     PVITEDDTAHFQLFRWGLVPFWAKDAKIGSKMINARLESIEEKPVFSKLLQAHRCIVP
                     FDGFYEWKLYPDKHREPYRITVRDGEIFSVAGLWSKWKDPEGQTLKTFTVITHAANRL
                     MKDIHDRMPAILTQEAERLWTDSDLSAKDALQLLVPFPAEEMQAYRVSDRINKVANND
                     AGLIEKFSNVDKPGNIQDTLF"
     gene            complement(4050..5228)
                     /locus_tag="PLE23_09460"
     CDS             complement(4050..5228)
                     /locus_tag="PLE23_09460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HMX83531.1"
                     /translation="MSQYPTLVLKSGRDRSVINRHPWIFSGAVKELPKANTGNIIRVT
                     DNQHTLLGYGFFDPKSQITCRIFEFTKEERSFDHTYWTEKLTAIHTWKTALFDPKQTN
                     AYRLVHAEGDHFPGIIVDIYDDTAVVQILIKGTENLKDEIGSILLNLGFSYVYFRIKQ
                     STGKLEDVAEQSHWYSEPRSMPIEIIENGIRMLVDVKKGQKTGFFLDQRENRRIVGEL
                     SKGKKVLNTFSYTGGFSLYALAGGATLVDSVDSSAAAIELCDENVRLAFGDAPHRSFT
                     EDVFDFLKKMETDYDIIILDPPAFAKNARSVENAAKGYKNLNLQAMRKIKNGGLIFTF
                     SCSQHIDKELFRKIVFGAAADAARPVTILRQLTQPEDHPINIFHPESEYLKGLLLRVG
                     "
BASE COUNT         1494 a         1010 c         1326 g         1510 t
ORIGIN      
        1 aggtaaggga tattagccgg cacgaagtaa ggcggatagc ccgacccgac aggggtacgc
       61 ccaaataaat gaaagaacgc aacgtaatga aaaatatagt tgcgaaatct tggtgttgtc
      121 gttattgtca ggtatcttca tggctgcatg acaaaaaaca ggaagtgatg gatatgaaac
      181 atgtgataat agtgagtaat ctggtgataa cggtgttggc accgctggtg gctgtgtatg
      241 cggtggggct ggcgaggcaa gggcaacata ccaggcatct gcgtgtacaa aaagtgctgt
      301 ttggcgtgtg tatgtcgatg ttggtgatat tcgaactgct gatacgggca tcgggtgggt
      361 cgggtagcct ggtggagcat agtacacact atgggcagtg gtattttaag ctgatactgg
      421 tgtcgcacat agtaggtgcg gtgttgacgt acatcatctg ggcagtgatg gtggtgctgg
      481 ctagccgaaa gtggaagcgc gggcagctgc cggggaagtt ttctgtgaca cacaaacgac
      541 ttggttatgt aacgatgatc gggctgtggt acacggcggt cacggcagta atagtgagtg
      601 tactagcttt ttttgggtaa attattttgt tttctgttat ctgcgtgcta tttttgagga
      661 attgaaccaa gaatgcaaag gtgttagcta aggaaaatgt gttgttggac cggacattga
      721 aaatattaaa taaatgaaaa atatactgaa ggtttttgtg ttatttgttt gcatgtatag
      781 cctgcctggc gagattttgg cacagggctg cagtgatgcc ggcctttgta cgctcaacag
      841 ctttaagccc aaagagcaat cggacttcgg gagccagttt aagttgggtg cttcccttgg
      901 taatgccgat atggcaattc agaccatagg tgcatatgcg gagttcgata agcagatagg
      961 tgctaagttt ggttttgatg ttaaactaac agccttatcg caaaacggga accagatatc
     1021 gaacttcgga ttgtctgact tgtatgtgaa tactaattac cgtatcggtg aaaaatcgag
     1081 attggcgctt ggtgtaaaga taccgctttc tgacggcaat gtgaaggaaa atgaaaagtc
     1141 gctacctatg gattaccagc caagtttggg tacctacgat ctgatattag gtgctactac
     1201 aagcctgatt gggattcagc tggctgctgc ggttcaattt ccgcttacac agaacaaaaa
     1261 cgagtatttt tctgctcaat atcctatagg tacgtcactt agtgcttttc agacaaccaa
     1321 caaattcaaa cgtagtggcg atataatgtt gagagtttca tatccgataa acctgggtcg
     1381 aaaactgagt attacgccaa gcatattgcc gatttaccat cttgcgaatg acaaatatac
     1441 agacataagc ggagcagaaa gagtgattgc cggctcacat ggtcttacgc taaacggcaa
     1501 tctttatttc gactatctgt tgggagataa aagtgctgtt cagctcaatg tgggtgctcc
     1561 gttcgtggtg cgcgacaccc gaccagatgg ccttacgagg agttttattg ctacgctaga
     1621 gtataggttt acattctgag tttgccaagc tgtttgtaag ggggcgaggt agttttcaat
     1681 agtggtgtta gcttatcgtt tagcctcaat gaagagaaat cgtaaggaaa tgatgcccat
     1741 aacggcctta gttggttata ggaaggcata tttgatagga tagggtgaat gttgttgatt
     1801 gctcaaggac aaaggcaggt atttttcagg atatacagta taagtttcgc agcgtatttt
     1861 atgacgaaat tatttgtagc taatgatata tctgcgtatt ttttctttgt tatgaagttg
     1921 gtttttatta gaagttgaag ttcgggtgct tgttaagaat gaatgtgtaa acgggaaaca
     1981 aatttgaaca atcagtgatt atttacatta aaatatagaa aaaataagga gaaaagcgca
     2041 tgcatgaact ttggacgaaa atattgagct ataccacact tcgtgaccgg gagaaacgga
     2101 ggaaatataa atacggttat tcggtttatg tacttgccaa ggctcaacga gagttcagga
     2161 ttttgctcaa gcgatatgtt aaagacagca tattgataac agcaggcata ttttcagctg
     2221 catttggctt gaagggtttc ctgcttacca atcatttcat tgatggtggt gctacgggca
     2281 tatcgctatt gttgtctata gtgctgaaga tgcccttacc attgttcatt attctggtca
     2341 atattccttt tatatatctg gcttacaaaa cagttggtaa aatatttgcc attaaaacga
     2401 gtcttgccat caccgggctt tcaatcgtgc tggctaccgt cactttcccc aatgtaacag
     2461 acgacaatct acttgtggcc atcttcggtg gtttttttct gggggcaggt ataggtctgg
     2521 cagtgagggg aggcagtgtg attgacggaa cagaagtact cgccatctac ctgagccgga
     2581 agttgagtac aacgcttggt gatattatta ttctcatcaa cattattatt ttctctgctg
     2641 cagcatattt tctctcgata gaggctgcca tgtattcaat gattacatac ctgtcagcat
     2701 caaagacact tgacttcata gcagaaggta tcgaggaata catcggagtc accattatat
     2761 caaaacaatt tgaaaaaata cgccagatga tcgccgaaag gatgggaaga ggtctgacgg
     2821 tctataacgg caaacgcggg tacggaaaat cgggcgatac gcttgagata gacattatct
     2881 ttacggtggt cacaaggctt gagctaagta aactgaatgt ggagctcgaa aaaattgacc
     2941 ctgacgcatt tgtcatcatg agtgccataa aggataccaa aggtggcatg ataaagcaaa
     3001 ggccactgaa acattgatgt aaccgaaaag atgccgaaat accgggtctg ttgaagaaag
     3061 aattgcagtt ttacaaaata aaaaaaatcc tgcactaata tgagggtaag aggtgcagga
     3121 tcaatcaaca aacatactat gagaaaaccg atgtaaagat gagacgaaat acggcgtctg
     3181 gcaagtggaa atgacgtaac ggcatgaata agaatgtaac ggtcattccg gtttagaaaa
     3241 cggcagatgt ggattttatt ttgtgtctga tttacattga aacggttttg gtatgtaatt
     3301 ttacccaaca aaaagaaaat gccactatgt gtggaaggtc ttctctaacg aaaaatgaaa
     3361 aggaactcga gcagcgtttc ggcgccacct tttacagcga cgagttgctc cggtataatc
     3421 cgttaccaaa ctacaatgtt gcacctacgc attatcatcc ggtcataacg gaagacgata
     3481 cggcacactt tcaactattc aggtggggtc tggtaccttt ctgggcaaag gacgccaaaa
     3541 taggcagcaa aatgattaat gcacgactcg aatctataga ggaaaagccg gtttttagca
     3601 aattgcttca ggcgcacagg tgtatcgtac catttgatgg attttatgaa tggaaattgt
     3661 accctgacaa gcaccgtgaa ccttaccgta ttacggttcg cgatggcgaa atattttcag
     3721 ttgcagggct ttggtctaag tggaaagacc cggaaggaca aacgctcaaa acctttacag
     3781 tcataacgca tgctgccaac cgtctcatga aggatataca cgaccgtatg ccggccattc
     3841 tcacccaaga agccgaaaga ctttggacag actctgacct gtcagccaaa gatgcacttc
     3901 agttactggt accttttccg gccgaagaaa tgcaggctta tcgcgtgtcg gaccgtataa
     3961 acaaggtagc caataacgat gccgggctga ttgagaaatt ttcaaacgtt gataaaccgg
     4021 gcaacataca ggacacactg ttctgaacac tacccaactc taagcagcag ccctttgagg
     4081 tattcgcttt cgggatgaaa tatattgata gggtggtctt caggttgggt cagttgtctg
     4141 agtatggtaa caggcctggc agcatcggca gcagctccga acacgatttt cctaaacagt
     4201 tctttgtcga tgtgttgtga acaagaaaac gtaaaaatca ggcctccgtt tttgatttta
     4261 cgcattgcct gtaggttgag gtttttatag cctttggcgg cattctctac cgaacgtgca
     4321 ttcttggcaa atgccggtgg gtcgaggatg atgatatcgt agtctgtctc cattttttta
     4381 aggaaatcaa agacatcttc tgtaaaagag cggtgaggtg catcaccaaa ggcgaggcgg
     4441 acattttcat cacaaagctc tatggcagcc gccgaactat caaccgaatc aaccaatgtg
     4501 gcgccacctg ccagggcata cagactgaat cctccggtgt aactgaaggt gttgagtact
     4561 tttttgcctt tgctcagctc gcccactatc cggcgatttt cacgctgatc gaggaaaaac
     4621 ccggttttct ggcctttctt aacatcgacc aacatacgga tgccattttc aataatctct
     4681 atcggcattg atcttggttc gctataccag tgcgattgtt ctgctacatc ttcgagctta
     4741 cctgtactct gttttatcct gaaataaaca tagctaaatc caagattaag aagtattgaa
     4801 ccgatttcat ctttcaggtt ttctgttccc tttataagta tttgtactac agcggtgtcg
     4861 tcataaatgt ccactattat tcccgggaaa tgatcgcctt cggcatgtac gaggcggtag
     4921 gcgttggtct gttttggatc gaacagggcg gtcttccagg tgtggatagc tgtcagtttt
     4981 tcggtccagt atgtgtggtc gaatgagcgt tcttctttgg tgaattcaaa tatacggcag
     5041 gtgatttgac ttttagggtc aaaaaaaccg tatccgagca aggtatgttg attgtctgtt
     5101 acacggatga tgttgccggt attggctttg ggcagttctt tgactgcacc tgagaaaatc
     5161 cacggatggc ggttgataac agagcggtct ctgcccgatt tgagtaccag ggtagggtat
     5221 tgtgacatta ttttattttg aattcgatta aatctctgat aataacggat gcgcaggcac
     5281 caccgaatgc tgccggtaga taagagatgg tgccgtatgc cgattttttg taatcgagtc
//
LOCUS       DAOQQY010000202         5331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6940_length_5331_cov_5.913192, whole genome shotgun sequence.
ACCESSION   DAOQQY010000202 DAOQQY010000000
VERSION     DAOQQY010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6940_length_5331_cov_5.913192"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1707)
                     /locus_tag="PLE23_09465"
     CDS             complement(<1..1707)
                     /locus_tag="PLE23_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83532.1"
                     /translation="MNNNKKILIYLSVAVGAMLALLADYHITNKISRSNFKTEIKTRL
                     DTSHKLAGEFAALYNQHTTEEFIRQYYHRIENLSSNHIQSLVFQNDSLIFWTSPPEIL
                     IDSSILHSTHNRFIFRNENVYEILKQQLSDIKYAVSVIKILPYNDPQLLVSTDSIAPK
                     DIRLETSDHSEKVFVQSKYVNHSKIHQYLRLSLFLICFITLLTLIKTVSSGLLKHFPE
                     WVVSVFILSGVFGLRLLFSMIDVSSYFGDISIFSKTLNSAYLGSSVGDFLLNALLFLW
                     VVIYWFNTFKAVSYEHLQHKARLILGTSIFFAIVLGIIMISGIIKSTILESDIYFDFE
                     NIFNLSIESLTLMLGLMIVFIALFLYSQRLMFTINLLELSSIQRIFCIFSSLLLLFVF
                     LQVVPVVDINAKFFLFILMYLLLLDFYSDTRTTDFTWLMIWLIIFSGFSAALLYTYNQ
                     SKEEVQLQQYSKILANPRDKYLEEQCRLLNIESERTKLYDLHSSLQSKLYISKFYNIR
                     DSFDANNISIWYPTQVNQLKWGILHNNGNTVYEYFHEGSKNYIYLETKDFMDFGTFSG
                     LEG"
     gene            1806..2184
                     /gene="rnpB"
                     /locus_tag="PLE23_09470"
     ncRNA           1806..2184
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLE23_09470"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2245..3447)
                     /gene="porV"
                     /locus_tag="PLE23_09475"
     CDS             complement(2245..3447)
                     /gene="porV"
                     /locus_tag="PLE23_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HMX83533.1"
                     /translation="MKKVVLLCTIAASMLLITTSLSAQTTPPDGCPLHINGKCINPLI
                     TAVPFLRIIPDARTAALGDAGIGASADPNAMHINSSKLVFADSKFAMAGSYSPWLRSL
                     GLTDVFLAYLSGYYKLDQLQTLGVGMRYFSLGNISFTDESGNPIRDGKPREFELSLGY
                     SRLLAENFSAGINGKFIYSNLAAGQVVESTDIFAGTAFAADLSLTYRKPIKMGETPTV
                     LTLGAAFTNIGSKITYTKSINKDFLPANMGIGAGWEFNLDEHNSLTLLADFNKLLVPT
                     PTTTDADGNGRPDYRDKSPIAGILGSFSDAPGGFSEEMREFSVSSGIEYWYDKQFALR
                     AGYYYEDPTKGNRQFLTAGLGVKYNVFSLNFSYLVPTTNQRNPLDNTLRFTFLFDIAS
                     FNKSAEAE"
     gene            complement(3541..>5331)
                     /gene="porU"
                     /locus_tag="PLE23_09480"
     CDS             complement(3541..>5331)
                     /gene="porU"
                     /locus_tag="PLE23_09480"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HMX83534.1"
                     /translation="TAINKIIQYDTNPDGMRDWRNRIVFSADDEDQNMHLDDTEGIAK
                     KITVTHPVYNQNKIYLDAFQQVATPGGDRIPEANNAINNDIFKGSLVYCYLGHGGPKG
                     LAQERVVNISDINSWTNFDRLALFITATCTFTGFDDPSIYSGGEQTFNNPKGGAIALL
                     TTTRAVYAISNAALVRTVYDSLFQKTNCANPRLGDVCRSSKDNFAFSPNLSNARKFLL
                     VGDPSIQIAIPSGNVETTKINGHLTKSGVGDTLSALQKVSIEGRVLSCSGQLLDDFNG
                     KLYVTIFDKAKDYKTLAQDKGSVVTSFSLQKNVIFKGVASVEKGLFSFSFVVPKDIDY
                     KYGLGKISYYAENGTDLDAAGYYDKIVIGGTATNPISDDTPPVVNVFLNDENFVKGGI
                     TEANPVLLAKISDDLGINITGSSIGHDLTAVIDENTQGEIILNDFFESELNNPAKGTV
                     RYPLKDIAPGVHTLKVKAWDISNKSGEGTTEFTVAENGVLALAHVLNYPNPFTSSTSF
                     QFEHNFGNKNLHVRISIYSVSGSLVKTIHQDIQATGSRITGINWDGKDDYGNGLARGV
                     YIYKVKISETEGGVAGKKAESDYEKLVILK"
BASE COUNT         1578 a         1027 c         1068 g         1658 t
ORIGIN      
        1 cccttcaaga cccgaaaaag tgccgaaatc cataaaatcc tttgtctcta aataaatgta
       61 atttttactg ccttcgtgga aatattcata aacggtattg ccattattat gtaatatgcc
      121 ccatttaagc tggttgactt gtgtcggata ccatattgaa atattatttg catcaaatga
      181 atctcttatg ttgtaaaatt ttgaaatata tagcttgctc tgcaggctgg agtgcagatc
      241 gtatagtttt gtcctctcac tttcgatatt caataacctg cattgctctt caagatattt
      301 atcacgtgga ttggcaagta tttttgaata ttgttgtagt tgtacttcct ccttactttg
      361 attgtatgtg tataataatg ctgcagaaaa accagaaaaa atgattagcc atatcatgag
      421 ccacgtaaaa tctgtggttc tcgtatcact ataaaaatct aacagcaaca gatacatcaa
      481 aatgaaaaga aaaaatttgg cattgatatc gaccacagga acgacttgca aaaatacaaa
      541 caacaacaga agactgctaa aaatgcaaaa tatacgctgg atactgctta gctctagcag
      601 attgatggta aacatcagtc gttgactgta cagaaacagg gcaatgaata cgatcatcag
      661 gccaagcatt aatgtcagac tctcaatgct tagattgaat atgttttcaa aatcaaaata
      721 aatgtccgat tcaaggattg tgcttttgat gatacctgat atcataatga tacctaatac
      781 aatagcaaaa aaaatggatg tgcccaagat gagtcgtgcc ttatgctgta ggtgctcata
      841 cgaaacagct ttgaatgtat tgaaccaata aatgacgacc caaagaaaaa gaagcgcatt
      901 cagtaaaaaa tctccaactg aagatcctag ataagcagag ttaagcgttt tgctgaatat
      961 gctgatatct ccaaaatacg atgaaacatc gatcatagag aacaaaagcc tcagaccgaa
     1021 tacacctgaa agtataaaca cagatacaac ccattcagga aagtgcttca acaatcctga
     1081 tgaaactgtc tttatcaaag taagtaaggt gataaagcat atcagaaata aactaagcct
     1141 aaggtattgg tggattttac tgtggtttac atatttactt tgtacaaata ctttctctga
     1201 atgatcagaa gtttcaagcc taatgtcttt tggagctata ctgtcagttg atacaagcag
     1261 ctgaggatcg ttgtagggta atatttttat aacagaaacc gcatatttga tatccgatag
     1321 ctgttgttta aggatttcat atacattttc gttgcgaaat ataaatcggt tatgtgtact
     1381 gtgtaaaatg gaagagtcta taaggatttc aggagggcta gtccagaaaa tcagactgtc
     1441 attctgaaat acaagggact ggatatggtt tgagctaagg ttttcaatgc ggtgataata
     1501 ttgtcgtatg aattcctctg tggtgtgctg attgtataat gcggcaaatt ctcctgctaa
     1561 cttatggctg gtatcaagtc gggtctttat ttcagtctta aaattgctcc ttgatatttt
     1621 gttagtaatg tgataatcag caagtaatgc caacatagca ccaactgcaa cggacaggta
     1681 gattagaatt tttttattgt tgttcacata agattatact ttaatatgag aagcaggttc
     1741 cataatatta gtagtatttt tatatattat ttgtaaatta agtaaatgat catcaacttt
     1801 gtatgtctgc tgaccgttct gtcgcttttg taaaaaaaga ggaaagtccg gacaacgtag
     1861 agcaccacac catctaacgg atgggttcat gagcagtcat gaaacagata gtgccacaga
     1921 aacaagactt tcacacacat gtgtgataaa ggtgaaaacg tgtggtaaga gcgcacgatg
     1981 gtatccggca acggctatca tgggtaaacc ttgtgggttg caatgccatg taaaccggag
     2041 ttccgaaagg agcagggttg ctcgccctgt atgatgcttc ggagggtagg cagctagatc
     2101 ttgtctgtga aggcaaggcc agataaatga cagaaatttc agttagctga aagacagaat
     2161 ccggcttata ggtcagcaga ttttttaaaa aaagccggtg attaaatctg ccggcttttt
     2221 tgatttaata tttttcaaca atcactattc agcttctgcc gatttgttga atgacgcaat
     2281 gtcaaacagg aatgtgaatc gcagcgtatt gtcaagcggg tttctttggt tggtagtagg
     2341 tactaggtaa gaaaagttaa gcgaaaatac gttgtacttt actccgagac cggctgtcaa
     2401 aaattgacgg ttaccttttg taggatcttc gtaataatat cctgccctta gtgcaaactg
     2461 tttgtcgtac cagtattcga ttccggatga aacgctgaac tctctcattt cttcactgaa
     2521 gccacccggt gcatcactaa atgagccaag gatacctgct atcggtgact tgtcgcggta
     2581 atcggggcgg ccattaccgt ctgcatctgt agtggttggt gtcggtacaa gtagcttgtt
     2641 gaagtctgcc agtaatgtta gtgaattatg ctcatcaagg ttgaactccc atccggcacc
     2701 aatacccata ttggccggta aaaaatcttt attgatagat ttggtgtatg ttatctttga
     2761 accaatattt gtaaatgcag caccaagtgt cagtacagta ggcgtttcac ccatttttat
     2821 tggtttgcgg taggtaagtg ataaatcagc cgcaaaggct gtacctgcaa aaatgtcggt
     2881 actctctaca acctgaccgg ctgcaaggtt tgagtagata aattttccgt tgataccggc
     2941 agaaaaattc tcagctagca atcgtgagta tccgagtgac aattcaaact cacgcggttt
     3001 gccgtctctt atcgggttgc cactttcatc cgtaaatgaa atattgccca atgagaaata
     3061 acgcattcct acacctaatg tctgaagttg gtcaagtttg taatatcctg acaaatatgc
     3121 aagaaaaaca tccgttaagc ctaaagatct caaccaaggt gagtaactac ctgccattgc
     3181 aaatttactg tctgcaaata caagtttcga cgaattgatg tgcattgcat ttgggtcggc
     3241 acttgcacct ataccggcat cacctaaggc agctgtcctt gcatcaggta ttattcgtag
     3301 aaaaggtaca gctgtgataa gcggattgat acattttccg ttgatgtgca atggacatcc
     3361 gtcaggaggg gtggtttgag cagatagtga tgttgttatc aaaagcattg aagctgcaat
     3421 ggtgcagagt aaaactactt ttttcattct cattattaga tgtgtttttt gatgtgctgt
     3481 ttagacatac attttcgatg taagccgtag tacagcattt ataactatta ttaagcaaat
     3541 ttattttaaa atcaccaact tttcgtagtc actttctgct ttttttcctg caactccgcc
     3601 ttcagtttca ctaattttta ctttataaat ataaacacca cgtgcaaggc cattgccata
     3661 atcatcttta ccatcccagt ttataccggt tattctgctg ccggtagcct gtatgtcctg
     3721 atgtattgtt tttacaagtg atccacttac tgaataaata cttatacgaa catgaagatt
     3781 tttgttacca aaattatgct caaattgaaa agatgttgag ctggtaaacg gatttggata
     3841 gttcaataca tgtgcaagag caagtacgcc attctcagct actgtaaact cggtggttcc
     3901 ttctccactt ttgttactga tatcccatgc ctttactttt aatgtgtgta cgccgggtgc
     3961 aatatccttt aacggatacc tcacagtacc tttggccgga ttgttcaatt ccgattcaaa
     4021 gaagtcgttg aggatgattt cgccctgtgt gttttcgtct attactgctg ttaggtcatg
     4081 cccgatagaa ctaccggtaa tattgatgcc aaggtcatct gatattttgg ctagcaatac
     4141 cggattggcc tccgtaattc cacctttaac gaaattttca tcatttagaa aaacattgac
     4201 caccggaggt gtatcatcac taattggatt ggttgctgtg cctcctatta ctattttatc
     4261 ataatatcct gcagcatcca ggtctgtacc attctcagca taataactga tcttgcctaa
     4321 gccgtatttg tagtcaatat cttttggtac cacaaatgaa aaactgaata gccctttttc
     4381 tactgatgcc actcctttga aaatcacatt cttctgaagc gaaaagctgg ttactacact
     4441 tcctttgtct tgtgcaagtg ttttataatc tttagccttg tcgaatattg tcacatatag
     4501 tttgccattg aagtcatcaa gcaattggcc tgagcaggac agtacacgac cttcaatact
     4561 caccttttga agtgcactta gcgtatcgcc tacaccactt ttggtaagat gtccgttaat
     4621 ctttgtggtt tcgacattac ccgaaggtat agctatctgt atggaaggat cgccaaccag
     4681 aagaaacttc cttgcatttg aaagatttgg agaaaatgca aaattatcct tcgagctgcg
     4741 gcatacatca ccaagcctcg ggtttgcaca attggttttt tggaaaagtg aatcatacac
     4801 ggtacgaaca agtgccgcat tggagatggc atatacagcc ctggtagtag ttagcaacgc
     4861 aatggcacca ccttttgggt tattgaatgt ctgctctcct cctgaataaa tactaggatc
     4921 gtcaaacccg gtgaacgtac aagtagcagt aatgaacaac gcaagtctgt caaaatttgt
     4981 ccagctgttg atgtctgaaa tattgacgac tcgctcttgt gccagacctt tgggtcctcc
     5041 gtgaccaaga taacagtaca ccagactacc tttaaatata tcgttgttga tagcattatt
     5101 agcctcgggt attctgtcac cacctggtgt ggcaacctgc tggaaggcat ccaaataaat
     5161 tttgttctga ttatagacag ggtgtgtcac ggtaattttc tttgctattc cttctgtatc
     5221 atccaggtgc atattctggt cttcatcatc tgctgaaaaa acaattcggt tcctccagtc
     5281 gcgcataccg tcagggttgg tatcatactg tattattttg ttgatggcag t
//
LOCUS       DAOQQY010000203         5320 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6957_length_5320_cov_5.970750, whole genome shotgun sequence.
ACCESSION   DAOQQY010000203 DAOQQY010000000
VERSION     DAOQQY010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6957_length_5320_cov_5.970750"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(87..710)
                     /locus_tag="PLE23_09485"
     CDS             complement(87..710)
                     /locus_tag="PLE23_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HMX83535.1"
                     /translation="MATKKKYYVVWNGLKPGIYDSWDECKKQVNAVEGARYKSFPTLN
                     EAEFAFREGAPAVIRKSATGMKTIAKPKILLPSVSVDAACSGNPGLMEYRGINNQDGV
                     EIFRKGPYRDGTNNIGEFLAIVHALALLKKKNFNDVPIYSDSVTAMGWVKKKKANTKL
                     EQTQHNAELFELLARAEEWLEKNTYSNKVIKWETSVWGENPADFGRK"
     gene            849..1616
                     /locus_tag="PLE23_09490"
     CDS             849..1616
                     /locus_tag="PLE23_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMX83536.1"
                     /translation="MIIDTHSHIYSPDFDDDRDEIIQRAKNNNIKKIFLPNIDSSSIE
                     MLYNTEKLYPGMCIAMMGLHPCSVKENYLTELNIVESELSKRSFSAIGEIGLDYYWDK
                     TFINEQKEAFIRQMNLAKNFDIPIVIHSRDSTEDVISLVRQEKSPKLRGIFHCFGGSV
                     EEAQQITDLGFLLGIGGVLTYKKSGLDQTLKHIDLRHIVLETDAPYLTPVPFRGKRNE
                     PSYITFVAEKLAEAKGMSLEEVCRITTENAENLFNPY"
     gene            complement(1690..>5320)
                     /locus_tag="PLE23_09495"
     CDS             complement(1690..>5320)
                     /locus_tag="PLE23_09495"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83537.1"
                     /translation="ALVHNPSSTVVYFLSYEGSFTATNGAANGQTSVDIGVLEVGTEP
                     AGMSLSLTGTGSDYPDFAWQSPASATPGQLNTGQNLVGGTGNPGGQPTVTFTESKTAG
                     ACPNEGTITRTWTATDVCGNSFTHTQVITVIDDTPPSFIEPLPEDVTVECDNIPAVPD
                     VFATDDCGPLIGASTPVVWINEIHYDNTGADVNEFIEIAGTAGVDLSSYSLVLYNGSN
                     GQFYSQTPLTGIIPNQTSGYGAIAFTYPPDGIQNGSPDGIALVQGATVIQFLSYEGIL
                     TAANGPAMGMTSTDIGVQEPSNTAVGLSLQLTGTGNEYADFNWIGPVPQSPGLINISQ
                     TISPVIIQASFTSSFTAGACPQAGTLTRTWTATDACGNASTYTQTITVVDTKAPVFVG
                     TLPQDITIECDQPVPPAPVLTATDNCDTNVPVVLNSVTTPGSCPQESIITRTYTASDD
                     CGNIATHTYKITIDDSKAPVFNGVLPQDITLECDLPTPPAPTITATDNCAQTINVVFI
                     TSAVQGKCPQEQVITRTWTATDPCGNTASHTYTITKIDTKPPVISNVDPDLVFNCDET
                     IPVHGVVTVIDNCDPDPKVTFSEVSTQSPHIQLCEHFTYTIKRTWTATDACGNKAVKT
                     QTIKVQDVTPPQLTMVPPSDITLECDESGDHACPMPLDDCDANATIVLSIEIAPLPGY
                     CPNSYVVHRSWLAGDRCGNTVTLTQNVVVIDSEAPDIKCPADIVKVSNIPTVVTWPEA
                     KAGDYCDGYTPTVQIAGPPSGSIFQPNTKTKIVYQATDECGNVSTCSFVVTVTDIGSG
                     PTGNKVSGELVTPDGKQIKGVEMTVSEDVNQFLTADGHYEFNNIPDAANISISPKKDI
                     NPLNGVNTLDLVHITNHILGKKPLDSPFKKLAADVNDDGKITTGDLLQIRKLILHVTD
                     KFEKKDSWTFVVKDPSFVDPYQPITWTLSDNKELKGINKDEIVDFIGIKTADVTWDAK
                     TSSANGAAEVRSAQLATLHTDDKLFVKGESFTVPVSLTNNGELKAIQFSMKFDTSKLT
                     LTDVAGLNELVKHDYFGLRYSQEGYITTSFDKLPSNGNETLFALTFTAKADGELKKSL
                     RVNSDLTPAQAYTLEEDAINVSIDFGAEMTALPQGEIVLFQNEPNPFENNSVIRLYMP
                     ESAFATLSILNVLGQEVSKIEKTFDKGLNEIQLSSSSLPAPGLYHYTLRCGDTVLTKK
                     LIFIR"
BASE COUNT         1446 a         1131 c         1113 g         1630 t
ORIGIN      
        1 caaagttagt caattatagt tataattgaa agcctattgc ataattcagg tttaatataa
       61 aaggctcatt tcattcaaca aatgaatcat tttctcccga agtcggccgg attttcgccc
      121 cacacgcttg tttcccattt tatgacttta ttgctgtagg tatttttctc cagccattct
      181 tcggcacggg ccaaaagctc gaaaagttcg gcattatgct gagtctgttc gagttttgta
      241 ttggcttttt tcttttttac ccaacccatt gcggtcactg agtcggaata aattggtaca
      301 tcgttgaaat ttttcttttt cagcaaagcc aaggcatgga cgatagccag aaattcgccg
      361 atattgttgg taccatcgcg gtaaggcccc ttcctgaata tttcaactcc atcctgatta
      421 ttgattccac gatattccat caacccggga ttaccactgc atgctgcgtc cacacttacc
      481 gaaggcaata atatttttgg cttcgcaatg gttttcatgc cggttgcaga ttttcttatc
      541 actgccggcg caccttctct aaaggcgaac tcggcttcat tcaatgttgg aaaactctta
      601 tatctggcgc cttctactgc atttacttgt ttcttacact catcccaact gtcataaata
      661 cccggcttca ggccgttcca aacgacataa tatttcttct tagttgccat attgatgcga
      721 agataaataa atgaagcata ccatttctga tgtttttggt gaaataagct caaaatcatg
      781 aactaatcat actcatattt gattccataa gtatttaaag ataattttgc acttcaattt
      841 ttaataaaat gattattgac actcattctc atatttattc gcctgatttc gatgatgacc
      901 gggatgaaat cattcagaga gctaaaaata acaacatcaa gaaaatattc ttgcccaaca
      961 tcgacagcag cagtattgaa atgctgtata atactgaaaa attatatccg gggatgtgta
     1021 tcgcgatgat gggcctacac ccttgctcgg ttaaagaaaa ttacctgacc gaacttaaca
     1081 tcgtcgaatc tgaactgtcg aaaagatcat tttctgccat aggcgaaata ggtctcgatt
     1141 attactggga caaaactttc atcaatgagc aaaaggaagc cttcatcagg caaatgaacc
     1201 ttgcaaaaaa tttcgacata ccgattgtta tccatagcag agacagcaca gaagatgtca
     1261 tttctcttgt aagacaagaa aagtcgccca agttgagagg tatctttcat tgtttcggag
     1321 ggagtgtaga ggaagcacaa caaataaccg accttggctt cctcctaggt atcggtggag
     1381 tgcttactta caaaaaatca gggctagacc aaacacttaa acacattgac ttaaggcata
     1441 ttgtcctgga aaccgacgca ccttatctca cacctgtacc tttcagagga aaaagaaatg
     1501 aaccttcgta tattacattt gtagctgaaa agctagccga agcaaaggga atgagcctgg
     1561 aggaagtatg taggataaca actgaaaatg ccgaaaatct attcaaccca tattgatgta
     1621 agtataaaag taacagcccg aaaatcattt gtttttcggg ctgttttttg ctttacatta
     1681 tcatttcaat tacctgataa aaataagttt cttcgtcaac acagtgtctc cgcatctgag
     1741 tgtgtagtgg tacagacccg gtgcaggcag gcttgaagag ctgagctgaa tttcattcaa
     1801 tcccttatca aaagtctttt ctatttttga tacttcctgc ccaagaacat taagtatgct
     1861 tagtgttgca aaggcagatt ctggcatgta caatctgatg acggaattat tctcgaaagg
     1921 gtttggttcg ttttggaaca acactatttc gccctgcggt aatgcagtca tttcagcacc
     1981 aaaatctatt gatacattga tagcatcttc ttcaagagtg tatgcctgtg caggtgtaag
     2041 atcagagttg acacgaaggc tctttttgag ttcaccatct gcttttgctg taaatgtaag
     2101 cgcaaacaaa gtctcatttc cgtttgacgg aagtttgtca aaactagtcg taatatatcc
     2161 ttcctgcgaa tatcttaatc cgaaataatc atgcttcaca agttcattca gcccggctac
     2221 gtcggtaagg gttaatttgg aagtatcaaa cttcatgctg aactggattg cttttagttc
     2281 gccattattt gttaatgaaa ccggtacagt aaacgactct cctttgacaa ataatttatc
     2341 atctgtatgg agtgtggcca gctgagccga acgaacttct gcagccccgt tggcacttga
     2401 ggtttttgca tcccaagtca catcagcagt ctttattccg atgaagtcaa ctatttcgtc
     2461 cttgttgatg cctttcaatt ccttgttgtc tgataatgtc catgttatag gctggtaagg
     2521 atctacaaaa gaagggtctt tgactacaaa tgtccaggaa tctttctttt caaacttatc
     2581 agtgacgtgg agtatcagtt ttctgatttg cagaaggtca cctgtagtta ttttaccatc
     2641 gtcattgaca tctgcagcca gtttcttaaa gggtgaatct agtggcttct tgccaagaat
     2701 atgattggtg atatgtacca aatcaagtgt attcacaccg ttgagcggat tgatatcttt
     2761 ctttggtgaa atactaatat tggctgcatc cggaatgtta ttgaattcgt agtgaccatc
     2821 agcagtgagg aattggttaa catcttctga cacggtcatt tccacacctt ttatctgttt
     2881 accgtccggt gttacaagct ctccgcttac cttattgcct gtagggccag aacctatgtc
     2941 ggtgactgtt actacaaatg aacaggtact tacattacca cattcatctg ttgcctgata
     3001 gacaattttt gtttttgtat ttggctggaa aatacttccg ctcggcggtc cggcgatctg
     3061 taccgtaggt gtgtagccat cgcaatagtc tcctgcttta gcttcaggcc atgtcacaac
     3121 agttggtatg tttgacacct tcacaatatc tgcaggacat ttgatgtcag gtgcttcact
     3181 gtcgataaca actacattct gagtcagtgt tactgtattt ccgcaacggt caccagccag
     3241 ccatgaccgg tgtactacat aagagttagg gcaataacct ggcaagggcg caatttcaat
     3301 actaagcaca attgtagcat ttgcatcaca atcatccaat ggcatcggac aagcatgatc
     3361 accgctctcg tcacactcaa gtgttatgtc ggatggtggc accattgtga gctgtggagg
     3421 tgtaacatcc tgtactttaa tagtttgtgt cttaacggct ttgttaccac aagcatctgt
     3481 agctgtccat gtgcgtttga tagtataggt aaaatgttca caaagctgta tgtgtgggct
     3541 ttgagtactt acttcactaa atgttacctt cggatcagga tcacagttat caatgactgt
     3601 cactacacca tgtacaggta tagtttcgtc acaattgaat acgaggtcag ggtcaacatt
     3661 gctgataacc ggtggttttg tatctatttt ggttatcgta tatgtgtgcg aagctgtgtt
     3721 accgcacgga tctgtagccg tccatgtacg agttatcacc tgttcttgtg ggcatttgcc
     3781 ttgtacagct gaagtaatga atactacatt tatggtctga gcacaattat ccgtggcagt
     3841 aatggttggt gcaggtggtg taggcaggtc gcattcgaga gtgatatcct gtggcaaaac
     3901 accattaaac accggtgctt tgctgtcatc tatagttatt ttgtaggtgt gcgttgcaat
     3961 attaccacaa tcatcacttg ctgtataggt tcttgtgata attgattcct gcggacaaga
     4021 accaggagtt gttacagagt tgagcaccac aggtacattc gtatcgcaat tatccgttgc
     4081 agtcaagacc ggagcaggtg gaaccggctg atcgcactct attgtaatat cctgtggtaa
     4141 agttccaaca aacacgggtg cctttgtatc aacaacagta atagtctgag tatatgttga
     4201 tgcatttccg caagcatcgg ttgcagtcca tgtacgtgta agtgtacctg cctgaggaca
     4261 agctccggct gtaaaacttg atgtgaagct tgcctggatg attacagggc taatggtctg
     4321 actaatatta atcagacccg gactttgagg tacaggacca atccagttga aatcggcata
     4381 ttcattaccg gttccggtca gttgcaatga caaccctact gctgtatttg atggttcctg
     4441 aactcctata tctgttgaag tcatacccat ggcaggaccg tttgctgcgg taagtatgcc
     4501 ttcgtagctc aagaattgga ttacggtggc accttgtacc agtgctatac cgtcaggcga
     4561 tccattctga atgccatcag gtgggtatgt aaaggcgatg gcaccatatc ctgaagtctg
     4621 atttggaata atgcctgtaa gtggtgtctg gctgtaaaat tgaccatttg atccgttgta
     4681 cagtactaat gagtatgagc tcagatcgac acctgccgta ccagctattt caataaattc
     4741 gtttacatca gcaccggtat tgtcataatg tatttcattg atccaaacta caggcgtact
     4801 tgctccgatt agtggaccgc aatcatcagt agcaaacaca tccggcactg caggaatatt
     4861 gtcacactct actgttacat cttccggcaa tggttcaatg aaagaaggtg gtgtatcgtc
     4921 aatgacagta atcacttgtg tatgtgtaaa tgaattacca catacgtcgg tagctgtcca
     4981 tgttctggtt atggtacctt cgttagggca ggctcctgca gttttgcttt cggtgaatgt
     5041 tacagtaggc tgaccacccg gattacccgt tccacctacc aaattctgac cggtattgag
     5101 ctgacccggt gtcgcagatg caggtgattg ccatgcgaaa tccggataat cggatcctgt
     5161 gccagtaaga ctcaatgaca ttccggcagg ttctgttcct acctccaaaa caccaatatc
     5221 tacactcgtc tgaccatttg cagctccgtt agtagctgta aaagagcctt cgtagctcag
     5281 gaagtaaact acggtacttg aaggattatg aaccagtgca
//
LOCUS       DAOQQY010000204         5310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6979_length_5310_cov_6.107326, whole genome shotgun sequence.
ACCESSION   DAOQQY010000204 DAOQQY010000000
VERSION     DAOQQY010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6979_length_5310_cov_6.107326"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="PLE23_09500"
     CDS             <1..1494
                     /locus_tag="PLE23_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83538.1"
                     /translation="AKSDLNLYEYPLDREVIEMGDYYKEPGSPKDQISPFYAVVTPSQ
                     ALPNLSYEILSELHLRSNDEDLVRRALERVGYDPDVVGYILPGEPGDNGGSGGSEFEI
                     NSCGCHVYKEQRMPGGCIQVKDVEKDIFQGVRRVKVILKDTWFTEDETWTDDNGCFRI
                     NNKYYGKAWMWVKFKSPRVQIRGARSGFAAVWEWAFPVKDYVGQVSGPRFNDIQVKYD
                     IWSNQGSSAHIHWGAATVNNALHEFHDYAIADGINPPPPFLDIYVGRNHSYGYALMSG
                     QNMIAQAVFIVLIQTSYFAGPFGPLIAIIGASAVQSYLPDVYVGIEFNNSDRLKWLAY
                     HEIAHASHWTKAGVSFWNELVNAEVAAKGHGNAGSNNAGLISLVESWAEHIGLSYSHR
                     TYETATSYYITFIERLERTRNESPNHIPIGFYHDLIDNVPDIVNACDVQGGGCGPIND
                     QASGFTNSQMFGLLTSGTKSPLNFKDKLIQGASPATQTQVNNLFNSY"
     gene            1557..2246
                     /locus_tag="PLE23_09505"
     CDS             1557..2246
                     /locus_tag="PLE23_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83539.1"
                     /translation="MKAIITISFLTILSFSSCIKQENCPRYFQVPFDLSPINIEYHVG
                     DTLKVISKFSRHVLAYNGERNAIGRFDMKGILWKPVSVLFRIDSIEIKDNPRYSDFTS
                     NFEYIKQVDMNYNVTPQTDYGNMLWGEYQYSNDTFYLSYNVVCKKPGIYLLENSSDII
                     SGHGAQDFPGSCSKRNGFDVWVLMNGGINNHVELLKESPDPHWNTWLVQQEKDNKLFS
                     ELGGYCFKVIP"
     gene            complement(2230..2511)
                     /locus_tag="PLE23_09510"
     CDS             complement(2230..2511)
                     /locus_tag="PLE23_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83540.1"
                     /translation="MCENIPRIVKIKAINGFFSLEVEYYFFSTCPMTMYRRKIPDGRQ
                     KNTVFLTYSNQLGNLQSFEKYITDCELIMTEYLQERTSQVMTVGLRVLP"
     gene            2625..4427
                     /locus_tag="PLE23_09515"
     CDS             2625..4427
                     /locus_tag="PLE23_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMX83541.1"
                     /translation="MSDSAANQNVLYGGQFLIKDPDVENTFIPEEFNEEQLMVKQMTE
                     DFMKNTFEPVREKVEKMEDGVTVNLLHEAAALGLLGTHMPASYGGTELDTNTNTLINE
                     ILGSASSWIVSYAAHTGIGMLPILYFGTEEQKSKYLPRLISGELKAAYCLTEPGSGSD
                     ALAAKTRADLSEDGKYYILNGQKMWITNAGFADVFIVFAQIDGNKFTGFIVERNTEGI
                     TLGAEEAKMGIKGSSTRQVFFENVKVPVDNLLGEAGKGHKIAFNVLNIGRFKLAVLCM
                     GGSKEVVSMSTRYANERVQFKQPIANFGAIKYKLAEMATKLFTLESSVYRVSDQMEDL
                     KNKLQSQGESYSVAINQAAEEYAIECSVLKVFGSEVLDYVVDENVQIHGGIGFSEEFG
                     AARAYRDARINRIFEGTNEINRLLIVDMLLKRALQGKIDLVGPAWEVQKELASMPAFE
                     TLEGQFAQEHKAVKDFKKIILMVAGAAVKMQMDGKLNLEQEQELLMNVADLIIDAYAA
                     ESLLLRVEKLQVTGKAKLDMKVYEAMLSTFINDATHRMVKNGTDALCAFADGDLLKTL
                     LMGLKRYSKYPPVNVKEKRRIIADALIAHNEWCF"
     gene            4516..>5310
                     /gene="moaA"
                     /locus_tag="PLE23_09520"
     CDS             4516..>5310
                     /gene="moaA"
                     /locus_tag="PLE23_09520"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMX83542.1"
                     /translation="MPNDENNGNNILTDTFNRHHDYLRISLIDKCNLRCRYCMPSDLK
                     TTYSKTQLMSPDEILSIAQIFVNHGVKKIRLTGGEPLVRKDFGEIVEKLSTLPIKLCL
                     TTNGILLDRYFDVMKTKGLRSVNVSLDTLHKERFIHITQRDEFDKIYSNILQLVDEGF
                     QVKVNVVVMKNVNDDELCDFVELTRELPLHIRFIEFMPFDGNAWSQNKVVNYAEMLTA
                     ISEKFDFEKLEDPAHSTSKKFRVKEYKGTFAFITTMSTPFCSDCNRL"
BASE COUNT         1582 a         1009 c         1214 g         1505 t
ORIGIN      
        1 gcgaaaagcg atctgaatct gtatgaatat cctttggaca gggaagtaat tgaaatggga
       61 gattattata aggaaccagg tagccctaaa gatcaaattt cgccattcta tgcggttgta
      121 accccgtctc aggcactccc gaacctttcc tatgaaattt tatctgagct tcatttgcgg
      181 tcgaatgatg aggatctggt aagacgtgcg cttgaacgag taggctatga tccggatgtg
      241 gtcggatata ttcttcccgg agaaccagga gataatggtg gtagcggtgg ttctgaattc
      301 gaaataaatt cctgtggctg tcatgtttat aaggagcaac gcatgccggg aggctgtatt
      361 caggtcaagg atgttgaaaa ggacattttt cagggcgtga ggagggttaa ggtaattttg
      421 aaagacacct ggtttaccga agacgagact tggactgacg acaatggctg ttttcggatc
      481 aacaacaaat attatggcaa agcatggatg tgggtgaagt tcaaatcgcc gagggttcaa
      541 atccggggtg cgagatcggg ctttgcagcg gtgtgggagt gggcatttcc tgttaaggat
      601 tatgttgggc aggttagtgg tccccgattc aatgacattc aggtaaagta tgacatttgg
      661 agcaatcaag gctcctctgc gcatatacac tggggtgcgg caacggtcaa taacgccttg
      721 cacgaattcc acgattatgc gattgctgac ggcatcaatc caccgccgcc ttttttggat
      781 atctacgttg gtcggaatca tagctatggt tatgcattga tgtcggggca gaatatgatc
      841 gcacaggccg tgttcattgt cctaattcaa acgtcctatt ttgcagggcc gtttggccca
      901 ttgattgcaa ttatcggagc gtcggccgtg caatcttatc ttcccgatgt ttatgttgga
      961 atcgagttta acaactcgga tcgtttaaaa tggctggctt atcatgagat agcccacgca
     1021 tcgcattgga caaaggctgg cgtaagcttt tggaatgagt tggtgaatgc agaagtggcg
     1081 gctaagggtc acggcaacgc cggcagcaac aatgccgggc ttatttcgct tgtagaatca
     1141 tgggcagagc atatcgggtt aagttatagt catcgtactt atgagacagc cacatcttac
     1201 tacataacgt ttatagaaag attagagcgc acaagaaatg aatctccaaa ccatatacca
     1261 attggttttt accatgattt aattgataac gttccggata tcgtaaacgc ctgtgacgta
     1321 caaggcggag ggtgtggccc aataaatgac caggcaagcg gattcactaa ttcccagatg
     1381 ttcggattat tgacctccgg tacaaaatct ccccttaact ttaaggacaa actgatccag
     1441 ggggcttcac ctgctactca aacccaggtc aataatttat tcaacagtta ctaactttat
     1501 taatcaatgg caggcgtggt taatcttaca tgtctgctcc ttaattcttc ccgaatatga
     1561 aagccataat aaccatttca tttttaacaa ttttgtcatt ttcttcatgc ataaagcaag
     1621 aaaactgccc caggtatttt caagttcctt ttgatctgtc acccataaat attgaatatc
     1681 atgtcggaga taccctgaag gtaatctcta aattttcgcg tcatgtactg gcatataatg
     1741 gagaaagaaa cgcaatagga agatttgaca tgaagggaat attatggaag ccggtatcag
     1801 tccttttcag gatagattca attgaaatta aagacaatcc cagatattca gattttacat
     1861 caaattttga atacatcaaa caagttgaca tgaattataa tgtcacccca caaactgatt
     1921 atggaaatat gctttggggc gaatatcagt attcaaatga cacattttat ttatcgtata
     1981 atgtggtatg caaaaaacct ggtatttatt tacttgaaaa tagttctgat attatttctg
     2041 gtcatggcgc tcaggacttt cccggcagct gttccaaaag aaacggattt gatgtatggg
     2101 tactaatgaa cggaggaata aacaaccacg ttgaattatt aaaagaatca ccagatcctc
     2161 attggaatac atggcttgtt cagcaagaaa aggacaataa attgttttcg gagcttggag
     2221 gctattgttt taaggtaata ccctgagccc taccgtcatc acctgacttg tccgttcctg
     2281 caagtactca gtcataatca attcacaatc agttatgtat ttttcaaaac tttgtaaatt
     2341 gccaagctga tttgagtaag tcaggaaaac tgtatttttc tgtcttccat ccggaatctt
     2401 tctgcgatac atcgtcatgg gacaagtaga aaaaaaataa tattccactt ctaatgaaaa
     2461 aaatccattg attgccttaa ttttaacgat tcgcgggatg ttttcacaca cacacggctg
     2521 ttttttgcga aaattcgcta actttttaat cgcataattt gttttatatg tatggctaca
     2581 ttatctttgt agcgaaattt cgctataact ttaaaaacaa gcttatgtca gactcagcag
     2641 ctaaccagaa tgtactttat ggaggtcaat ttttgattaa agacccggat gtagaaaata
     2701 ctttcatacc cgaagaattc aatgaagaac aactgatggt caaacagatg accgaagatt
     2761 tcatgaagaa tactttcgaa ccggtacgtg agaaagtgga gaaaatggaa gatggcgtta
     2821 ccgtcaatct acttcacgaa gctgccgcac ttggattgct gggtacgcac atgccggcaa
     2881 gttatggcgg tactgaactt gataccaaca ccaacacgct catcaatgag attctcggtt
     2941 ctgcgagttc atggattgtg tcttatgctg cacatacggg gattggtatg ttacctattc
     3001 tgtatttcgg taccgaagag cagaagagta agtatctgcc aaggcttatt tcgggtgaat
     3061 tgaaagcggc ctattgtctt actgagccgg ggtccggttc ggatgcactt gctgctaaaa
     3121 ccagagcaga cctaagcgaa gacggtaagt attatattct gaatggacag aaaatgtgga
     3181 ttaccaatgc cggattcgct gatgttttta tagtttttgc acagattgac ggaaacaaat
     3241 tcaccggatt tattgtagag cgaaacacag aaggtatcac attaggcgca gaagaagcca
     3301 aaatgggtat caaaggctca tctactcgtc aggtattttt tgaaaatgta aaagtgccgg
     3361 ttgataatct ccttggcgag gcaggcaaag gtcataaaat cgcatttaat gtactgaata
     3421 tcggccgttt caagcttgca gtcttgtgta tgggtggctc taaggaggtt gtatccatga
     3481 gtaccagata tgccaatgaa agagtacagt tcaagcagcc tatcgctaat tttggcgcta
     3541 taaaatataa actcgccgaa atggcaacga agcttttcac actggaaagt agtgtgtatc
     3601 gggtgtcgga tcaaatggaa gacttgaaga ataaacttca gtcgcagggc gaatcttatt
     3661 cagttgccat taatcaggct gctgaggaat atgccatcga atgttcggtt ttgaaagttt
     3721 tcgggtcgga agttcttgac tatgtcgtag atgaaaatgt gcagatacac ggcggaatag
     3781 gattttctga agaattcggt gctgctcgtg cttaccgcga tgccagaatc aataggatat
     3841 tcgaaggtac taacgaaatc aaccgcctac tcatagtaga tatgttgctg aagcgtgcgc
     3901 tacaaggcaa gatcgacctc gtaggtcctg cctgggaggt acagaaggaa cttgcttcta
     3961 tgccggcatt cgaaacactt gaaggccagt tcgctcagga acacaaggca gtgaaggatt
     4021 tcaagaaaat aatcctgatg gtagcaggtg ctgccgtcaa gatgcagatg gatggcaaac
     4081 ttaatctcga acaagaacag gaactgctga tgaatgtagc tgacctgatc atcgatgcct
     4141 atgcagcaga atctcttttg ctaagggtag aaaaactgca ggttacaggc aaagctaagc
     4201 ttgatatgaa ggtgtatgaa gctatgttga gtacatttat caacgatgcc acacaccgca
     4261 tggtgaaaaa cggtaccgat gcgctgtgtg cctttgcaga tggcgacctg ctcaagacat
     4321 tgcttatggg actgaagcgt tattcgaagt acccaccggt aaacgtcaag gaaaaacgta
     4381 gaataatagc agatgccctt attgcgcaca atgaatggtg tttttgagaa agcttcagct
     4441 ggcaagtatt caaacattta ctgttttgtt ctgtttaaat acctaacttt ggtcaggatt
     4501 tattaaacag gtgccatgcc aaatgatgaa aacaacggca ataacatatt gaccgatact
     4561 tttaaccgtc atcatgatta tctacggatt tcactcatcg acaagtgcaa tcttcgttgc
     4621 aggtattgta tgccttccga cctgaagacg acttatagca agacgcagtt aatgtcgcct
     4681 gatgaaattc tgtccattgc gcaaatattc gtcaatcatg gtgtgaaaaa aatccgactc
     4741 acaggcggag aaccattggt gaggaaagat tttggtgaaa ttgtagaaaa gctatctaca
     4801 ctacccatca agttatgcct tacgacgaat ggtattttgc tcgacagata ttttgatgta
     4861 atgaagacaa aagggcttcg gtcggtcaat gtgagtcttg acacattgca caaagaacgt
     4921 ttcattcata tcacccaacg tgatgaattt gacaagattt attccaacat cttacagctg
     4981 gtagatgaag gatttcaggt aaaggtgaac gtggtggtta tgaaaaatgt caatgatgac
     5041 gaattgtgtg attttgtaga attaacccga gagcttcctt tgcacatcag gtttattgag
     5101 tttatgccct ttgatggaaa tgcgtggtcg cagaataaag tagtcaatta tgccgaaatg
     5161 ttgactgcca tatctgagaa atttgatttt gagaaactcg aagatcctgc acatagtact
     5221 tccaagaaat tcagggtaaa ggaatataaa ggaacatttg cattcatcac cactatgtcc
     5281 acaccttttt gtagtgactg taaccgcctg
//
LOCUS       DAOQQY010000205         5308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_6986_length_5308_cov_6.608795, whole genome shotgun sequence.
ACCESSION   DAOQQY010000205 DAOQQY010000000
VERSION     DAOQQY010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6986_length_5308_cov_6.608795"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLE23_09525"
     CDS             complement(<1..458)
                     /locus_tag="PLE23_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83543.1"
                     /translation="MSRKILIGIYTDPQGYPPTLNAITELSTCFENIDVLYRPHIDGM
                     WAYPVNVNLHPSGKLMSARAQEQSRLYKKVFFFLMFTWRLLRLGLKHKPKVFLMYDNL
                     ALFSYFLVRPFFLLQHKIWFHSHDVHDIQMVRKFSIGWFSIKSEIRNFSKL"
     gene            complement(696..1064)
                     /locus_tag="PLE23_09530"
     CDS             complement(696..1064)
                     /locus_tag="PLE23_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83544.1"
                     /translation="MFKNKFSIGDILEGTNRKFNEAYHYVVFYAGNTDDDFCGGFITT
                     SPKYQNIPMKSEHFESGHKIGYNNSYLAAHKFIKLKDWGPYTKVGRLTREGIQFMEEK
                     IGELQAVLYTDYIKKLNLNK"
     gene            complement(1085..1477)
                     /locus_tag="PLE23_09535"
     CDS             complement(1085..1477)
                     /locus_tag="PLE23_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83545.1"
                     /translation="MAALKKGDRIATDEDLYRRGYNSPQKKYLNPDGSATSRVFKLRP
                     KDEGKLSVDLAKLTTKETAVLDSSKYNLFLINNSVVESIELFTVYDPLDEPAVVSHGL
                     ILGLDPEDDTKPAFLAKKAVKVYPMWPI"
     gene            complement(1479..1958)
                     /locus_tag="PLE23_09540"
     CDS             complement(1479..1958)
                     /locus_tag="PLE23_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83546.1"
                     /translation="MTVLTNSYISDMPDKKFALLAPLGYRVSRKMFRETVATMSVIVN
                     NAGINSRIIQNIIEYKNLEDNWDFDNAKAPTDKVIQTALYISRLLQKYGQKIYHSAPG
                     PNGEILLDIRHKNKSKSFEIIIYDHKTNIVFIPEDDVLPNQEDFDEKDVIRYISWLK"
     gene            complement(1939..2673)
                     /locus_tag="PLE23_09545"
     CDS             complement(1939..2673)
                     /locus_tag="PLE23_09545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04255 family protein"
                     /protein_id="HMX83547.1"
                     /translation="MSVDVKLKNAPLQEVVFEINWQPQYDDFGNEFDLGFDLAQGKFA
                     ELIREFFPVHKRLNTMQQLPEFGQPVHQYWTDDGTWPVVHHGPGILTIHQVEQNYIWK
                     DFRKLIDRIIKQLTRSYDASISYNKLNLLYMDAFDDLEAGFLNFLEDNLQTKISNGYD
                     MPGTLDQMSLTRSYVQESNIVLTHEFFTAVNSKTSQKAILMNISAEKELQKNDEPDKV
                     IEELHDICSASFKKILNKTYYDSLNQ"
     gene            complement(2894..3940)
                     /locus_tag="PLE23_09550"
     CDS             complement(2894..3940)
                     /locus_tag="PLE23_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HMX83548.1"
                     /translation="MKGNLLESQAQALVNIVNTVGVMGKGIALQFKNLFPANYRRYVK
                     ACKNDELKPGVLLVTEDESLLSGKKIIINFPTKTHWRLPSQYSYIESGLKELIRIIQD
                     MGIRSVAVPPLGCGNGGLEWTRVKEMMERYLSNVDAEIFIYQPDMQIKEINNKDNTEL
                     TPARAMLLAVMYESVRHAELVSEFAAEKISWFLQRFGAEQEFKLDFQPHIYGPYSGKI
                     RHVLHYLNGAYITGFESKDKKPFDEIGMITDAEQTVLDYLQQPGHEKYRSIVDQTKQF
                     LDGHYAVYNLELLSSVDYIRLKKNTQNTDDIIAAFNAWNDRKKKQFTNRRFIETVSAH
                     LDKYLYDIPVQESW"
     gene            complement(3954..4187)
                     /locus_tag="PLE23_09555"
     CDS             complement(3954..4187)
                     /locus_tag="PLE23_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83549.1"
                     /translation="MTQIDNIPHILENGISHPSGTSANPDYVSIGDTSLIRADIPFTF
                     IKKFIVYNEAAKEILLDFGVEDTMVVVNSGYYF"
     gene            complement(4247..4690)
                     /locus_tag="PLE23_09560"
     CDS             complement(4247..4690)
                     /locus_tag="PLE23_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83550.1"
                     /translation="MPCKPYSNCCDFAEKANRIVACTDVKSSTKYVYENSSLDTLSKY
                     RVDGCLINDDDSRCDYLLLNCTKKISYFIELKGSDLIRPVEQIERSIDKLHPDFNEYS
                     VEARIILTRVNTTDLKSTKLIRLESKLKRLNGKLIKRTRQWTEVN"
     gene            complement(4695..>5308)
                     /locus_tag="PLE23_09565"
     CDS             complement(4695..>5308)
                     /locus_tag="PLE23_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX83551.1"
                     /translation="IIKERRLLTQYDIDYNKTKLAEDIISKILKGKYQYDKDGEKIFF
                     DTNRYVKLNFSSSGQQESLWILLLLFIIILEKQNVFIVIEEPEAHLFPEAQKEISNLI
                     ALMSNVENSQVIITTHSPYILASINNLILANKVGSENAKVADRINQNLWIRSDKVYAA
                     LVANGQAKEIIDSELNIIQQEKIDSVSSTINHEFDFLFQFETV"
BASE COUNT         1536 a         1055 c          895 g         1822 t
ORIGIN      
        1 agttttgaaa aatttcttat ttctgatttt atcgaaaacc atccaattga aaattttcta
       61 accatttgaa tatcgtgaac atcatgacta tgaaaccaga ttttatgttg taataagaaa
      121 aatggtctta ccaaaaaata tgaaaataat gcaagattgt catacattaa aaacactttt
      181 ggcttatgtt ttaaacctaa tctcagcaat ctccatgtaa acatcaaaaa gaaaaaaacc
      241 tttttataca gtctagattg ctcctgagct cttgcactca tcaattttcc ggaaggatgc
      301 aaattaacat ttacagggta agcccacata ccatctatat gtggtctgta caacacatca
      361 atgttctcaa aacaggtgct cagctcggta atggcattga gcgtaggcgg atatccctgt
      421 gggtcagtat aaatgccgat gagaattttt ctactcatat tgtaagtggt caacaaaaaa
      481 cgagaataat attataaaac cgagcaggca ccatacacta aggcgatttt ctttttcatg
      541 atatccattc tctatatttt tctcgagata acaaaaataa ggctgcgttc atttacaaaa
      601 gctttcatgc ggtataaccg gtggggtaag tgattttggg taaactaaat acgtttatac
      661 taaaagctta gatggaactt tgataaagct atgagttatt tattaagatt aagttttttg
      721 atataatccg tatataatac agcttgcaac tccccaattt tttcctccat aaattgaata
      781 ccttcccttg ttaatctgcc gacctttgta tatggtcccc aatccttcag ttttatgaac
      841 ttatgagcag caagatatga gttattataa ccaattttat gacccgactc aaaatgttcc
      901 gacttcatgg gtatattctg atatttgggc gaggtggtta taaatcctcc acaaaaatca
      961 tcatcagtgt tgcctgcata gaaaacaaca taatgataag cctcattgaa tttccggttc
     1021 gtcccttcca atatatcgcc aatcgaaaac ttatttttaa acatggtgta tcagttgatt
     1081 aattctatat aggccacatt ggatatacct tcacggcttt tttagcaaga aaagcaggtt
     1141 ttgtgtcatc ctccggatct aaacccaaaa tgaggccgtg tgatacaaca gccggctcat
     1201 ccaatggatc atacacggta aacaattcta tgctttcaac caccgaatta ttgatcagga
     1261 aaagattata ctttgaagaa tccaggacag ccgtttcttt ggttgtcagc ttcgccaaat
     1321 caacagacaa cttgccctca tccttcggtc gaagcttaaa cactctcgaa gttgccgatc
     1381 cgtccgggtt gagatacttt ttttgtggag agttatagcc ccttctgtac aaatcttcat
     1441 cagtagctat cctgtcacct tttttcaatg cagccatttt acttaagcca actgatgtat
     1501 cgtattacat ctttttcatc aaaatcctcc tgattcggca aaacatcatc ttcaggaata
     1561 aaaacgatat tggttttatg gtcgtaaatg atgatttcaa atgacttgct tttgttttta
     1621 tgtcttatgt caagcaatat ttctccattc ggacctgggg cggaatgata aattttttga
     1681 ccatattttt gaagcaatct gcttatatac aaagctgttt gaataacctt atcagttggt
     1741 gccttagcat tatcaaaatc ccagttgtct tccaggtttt tatattcaat aatgttttgg
     1801 attattctgg aattaatacc ggcattattg acaattacag acattgttgc gactgtttcc
     1861 cgaaacattt tccttgacac cctatagccc aaaggagcca gcaatgcaaa tttcttgtcc
     1921 ggcatatccg aaatatagct attggttaag actgtcatag tacgttttat ttaatatttt
     1981 tttaaaactc gctgagcaaa tatcatgcag ttcttcgatc accttgtctg gttcatcatt
     2041 tttttggagc tctttctctg ccgagatatt cattaaaatt gctttctgag atgttttgct
     2101 gttcactgcc gtaaaaaatt catgtgtcaa aacaatatta ctttcctgaa catagcttct
     2161 ggtcaatgac atctgatcaa gggtccctgg catgtcataa ccattagaaa tttttgtctg
     2221 aaggttatct tccagaaagt tcagaaaccc ggcttccaaa tcatcaaaag catccatata
     2281 taacaaatta agcttgttgt agcttatgga tgcatcataa cttcgtgtga gttgcttaat
     2341 tatccggtct ataagttttc tgaaatcttt ccagatataa ttttgctcaa cttgatgtat
     2401 cgtcagaatc cccggaccat gatgtactac cggccacgta ccatcatctg tccagtattg
     2461 atgaaccggc tggccaaatt ccggtaattg ctgcatcgtg ttgagccttt tatgaaccgg
     2521 aaaaaactcg cggatcaatt ctgcaaactt accttgagcc agatcaaagc ctaaatcgaa
     2581 ttcattccca aaatcatcat actgtggctg ccagttgatt tcaaaaacga cttcctgtaa
     2641 tggtgcattc tttagtttaa catccacgct cattcctgat tatatatgat tgtataaagg
     2701 taaatattat tcgcttgaaa tcctaataat gcttggtatc actaaaaata tctacttcag
     2761 catcaataaa aatcgaaaac cagccccggt gcgctgatct gacgagaagc gtggctggta
     2821 tggttgttac caaatttaag gtattctttg ttgggttcat aatatttctg gctcttaata
     2881 aggctttaaa atgctaccaa ctttcctgaa ccggtatatc atagagatat ttatccaagt
     2941 gggctgatac tgtttctata aacctgcggt tggtgaattg cttttttttg cggtcgttcc
     3001 aggcattgaa tgctgcaatg atgtcatcag tattttgtgt atttttcttc agccttatat
     3061 aatccacact tgacaaaagc tccagattgt aaacggcata atgaccatcg aggaactgtt
     3121 tggtttgatc gactattgag cggtattttt catgacccgg ctgttggaga taatccagca
     3181 cggtctgttc tgcatcagtt atcatgccga tttcatcgaa tggcttcttg tctttagatt
     3241 caaaacccgt gatgtaagct ccgttgagat agtgtagtac atgcctaatt tttcctgaat
     3301 aaggcccata aatatgaggc tgaaaatcca gcttaaactc ttgttctgca ccgaaacgct
     3361 gcaaaaacca gcttatcttt tcggctgcga attcagatac caactccgca tgacgcactg
     3421 actcgtacat tactgccagc aacattgccc ttgcaggagt gagttctgta ttatccttgt
     3481 tattgatttc tttaatctgc atatcaggtt ggtatataaa tatttccgca tctacatttg
     3541 agagatatcg ttccatcatt tcctttaccc ttgtccactc aaggccccca ttgccacaac
     3601 ctaatggagg aactgctaca gagcggattc ccatatcctg aatgatacgg atgagttctt
     3661 tgaggccgga ttcaatatag ctgtactgcg aaggcaggcg ccagtgtgtc ttggtcggaa
     3721 aattgatgat tattttcttt ccactcaaca gggattcgtc ttctgtaaca agtaatacac
     3781 ccggcttgag ttcatcgttt ttacatgcct tcacatatct gcgataatta gctgggaaca
     3841 ggtttttgaa ctgcaatgcg atgcctttac ccatgacacc gaccgtattg acaatgttta
     3901 ccaaagcctg tgcctggctt tccagtaaat ttcctttcac ataatgcaac atatcaaaaa
     3961 tagtatccgg aattgactac aaccatagta tcttctactc caaagtcaag caatatttct
     4021 ttagctgcct cattgtaaac gataaatttt ttgatgaatg taaatggtat atcagcccta
     4081 atcaggctcg tatcaccgat agagacataa tcagggtttg cacttgtacc ggaagggtgt
     4141 gatatcccgt tttcaagtat atgcgggata ttgtctattt gtgtcatgcg ataaattata
     4201 gtatggaaac tctttatgta aaccagacgc ttattggcac ttatgttcaa tttacttcag
     4261 tccattgtct ggttctttta attaattttc catttaatct tttcaactta ctttccagcc
     4321 tgattagctt tgtgcttttc agatccgttg tattgaccct tgttaagatg attcttgctt
     4381 ctacggaata ttcattaaaa tccgggtgta acttatcaat tgaacgttca atttgttcaa
     4441 ctggtcttat taaatctgac cctttaagtt ctataaaata tgagattttt tttgtacagt
     4501 ttaatagtaa ataatcacat ctggaatcgt catcatttat taaacatcca tcaactctat
     4561 atttagataa tgtgtccaaa gaactatttt cataaacata ctttgtactt gattttacgt
     4621 cagtacaagc aacaatcctg tttgcctttt ctgcaaaatc gcaacagttt gagtaaggtt
     4681 tgcaaggcat agttttaaac agtttcaaat tggaataaaa aatcaaattc gtggttaatt
     4741 gtacttgaaa ctgagtctat cttctcttgc tgtattatat ttaattcgct atcgattatt
     4801 tctttggctt gtccgtttgc tactaatgca gcgtacactt tatcactcct gatccataga
     4861 ttttgattta ttctgtctgc cacttttgca ttctcagaac caactttatt ggcaagaatt
     4921 aaattattta tcgacgccaa aatatacggg ctgtgcgttg taataattac ctgtgagttt
     4981 tctacattag acatcaaggc aatcaagttt gaaatttcct tttgagcttc cgggaacaag
     5041 tgagcttccg gctcttcaat cacaataaac acattttgtt tttctaaaat aatgataaaa
     5101 agcaaaagga gtatccacaa tgactcttgt tgtcctgatg atgagaaatt tagcttcaca
     5161 tatctgtttg tatcaaaaaa tattttctct ccgtctttat catattggta ttttcccttc
     5221 aagattttac taatgatatc ttccgccagt tttgttttat tgtagtcaat atcgtattga
     5281 gttaagagtc ttctctcttt aattatgt
//
LOCUS       DAOQQY010000206         5290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7031_length_5290_cov_7.221203, whole genome shotgun sequence.
ACCESSION   DAOQQY010000206 DAOQQY010000000
VERSION     DAOQQY010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7031_length_5290_cov_7.221203"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLE23_09570"
     CDS             complement(<1..383)
                     /locus_tag="PLE23_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83552.1"
                     /translation="MSTLQYQYFLIGWMAFAIIIFIVLMFITAPYGRHSKTNWGPMVG
                     NRLGWVVMEMVVLLVLWYFIFKGKNEQTSANVLIISMFTFHYFNRSFIFPLRLKTTGK
                     KMPLAIMFMGIVLNIVSGFTFGYYFG"
     gene            complement(380..1372)
                     /locus_tag="PLE23_09575"
     CDS             complement(380..1372)
                     /locus_tag="PLE23_09575"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMX83553.1"
                     /translation="MSWKVAVIGASGQIGSALCRELVRQGYEVNALVRNDCPAINGLP
                     LKVCSGNVLDKDSLIRLMDECNVVIHTAANIELGYRFSQSLYNVNVTGAKNVLEAAEE
                     CGVQKVLLFSSVHVFDQKPSERILDESRNFVGNNSVFYDQTKREAHLLGMSAAEAGQD
                     VVILCPTSVVGPPDFKYSHIGKAVYDIYTGRVPAVVKGGFDFVDLRDVVQASINAIHH
                     GRSGEAYILGGKYHTVKEFADLIYAHKELERRMTELPASVAIAALPVVKFWTRLTGKD
                     SFYDKVYIDILQDGNRYISSEKAKKELRFSPRPLSETLEDLIAWFIEQGKLQAR"
     gene            complement(1640..2137)
                     /locus_tag="PLE23_09580"
     CDS             complement(1640..2137)
                     /locus_tag="PLE23_09580"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMX83554.1"
                     /translation="MSLTKSLIAEIKHESEGTKRMIQAVPAESFEWRPHAKSMKLKTL
                     ATHIVGLADWPELIAKMDELDLAGDTLKNIDVKTNDDLVAELEQRTQKSIETLQSVND
                     EDLNEIWTLRAGDRLILQLPKAVAIRSMALNHLYHHRAQLSVYLRLLDIPIPGLYGPS
                     ADEKA"
     gene            complement(2422..>3331)
                     /locus_tag="PLE23_09585"
     CDS             complement(2422..>3331)
                     /locus_tag="PLE23_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83555.1"
                     /translation="ATDANGCEVKLCCPVVIKEGNCIIPCAMGPVTAIAGACNPLTNE
                     YSVSGTVTFTNPPSTGTLTVTSGTKSQTFTAPFASPISYTLSGLVSDGLSHTVTAVFS
                     ADPNCAGSATYTAPVACECDKTQYEICPGESYTLTAQAGLSNYQWYTISGTDTTAISG
                     ATNLTYTTTTIGTYIWKATDANGCEVKLCCPVVIKPGNCLPCVTVTAIAENCASPEGK
                     YSLTGVVTFQTPPASGDLVIKVGPHTIIVPAPVTSPYAYVIPDLPADGAVHTVTAYFT
                     GNPDCVDSKNYTAPLKRCVPLQVIKN"
     assembly_gap    3332..3431
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3432..4336)
                     /locus_tag="PLE23_09590"
     CDS             complement(<3432..4336)
                     /locus_tag="PLE23_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83556.1"
                     /translation="MKWVGILLLVLLVQFKANSQVYYPICPGESYTLTAQSGLNNYQW
                     YSISGIDTTAIPGAANMSFTTSAPGTYIWKANNNSGCIVSLCEPVLIVTGTCLLPPDT
                     VYQICPDETYTLVAQTGLSDYQWFTLSGTTETMIPGATNISYIANAPGTYLWTAKDAN
                     NCLVKLCTPVQINASTSCAMCPDTTYQICPGESYTLTALAGMASYEWYTIVGSDTLVI
                     AGETNISYITSNAGTYIWKAKDVNGCDVSLCCPVKIVPGTACMPCPDTPYEICPGESY
                     TLTAQAGLSNYQWYTISGTDTTAISG"
     gene            complement(4673..>5290)
                     /locus_tag="PLE23_09595"
     CDS             complement(4673..>5290)
                     /locus_tag="PLE23_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83557.1"
                     /translation="GFQGATSFNIPANTEIKLFSFDNGGNCLGALQLFENFSDPFSNL
                     TPPDYANSANTNPGNAITIFGAGGDDYLGNYGAGANACPAIGMTIQNPPVQNALVNQP
                     KSGNSATELNPQGGNGPYTYSNGSGEPGCVAPSGATALPGASNFSINSTTGVYTYTAP
                     PVVGKYYYCVKVCDSSTPASCQVGVYTLNVTCVPYCSNVVTVQKN"
BASE COUNT         1524 a         1149 c          990 g         1527 t
ORIGIN      
        1 ccaaaataat aaccaaatgt aaacccacta acaatattta acacaatacc catgaacatg
       61 attgccaaag gcatcttctt gcctgttgtc tttaaccgaa gtggaaaaat gaagctcctg
      121 ttgaaatagt gaaatgtgaa catactaatt atcaatacat tagcggatgt ttgttcattt
      181 tttcccttaa atataaaata ccataaaaca agcaaaacta ccatttccat aacaacccaa
      241 ccaagtcggt tacctaccat cggcccccaa ttggtcttgc tgtgcctgcc atagggcgct
      301 gtaataaaca tcaatacaat aaaaataatg atagcaaatg ccatccagcc tatcagaaaa
      361 tattgatatt gcaacgtgct catcttgctt gcaatttacc ctgctcaata aaccatgcaa
      421 tcaagtcctc cagtgtttca ctgagtggtc ttggtgaaaa ccggagctcc tttttggctt
      481 tttctgaaga aatataccgg ttaccatctt gtaaaatgtc tatgtaaacc ttgtcataaa
      541 atgaatcttt acctgtcaat cttgtccaaa attttaccac aggcaatgcc gcaattgcta
      601 cagatgccgg taactcggtc attctacgtt ccagctcttt gtgtgcatat attaggtcag
      661 caaattcttt gactgtatga tattttccac caagaatgta tgcttcacca gacctgccat
      721 gatggatagc attgatactg gcttgtacta catcacgcag atcgacaaaa tcaaagccac
      781 ctttgaccac agccggtact cggccggtgt atatatcata aactgccttc cctatgtgcg
      841 aatacttgaa atccggtggc cctactactg acgttgggca aagaattaca acatcttgcc
      901 cggcctcagc agccgacata ccgagtagat gagcttcgcg cttggtttga tcgtaaaata
      961 ctgagttgtt accgacaaaa tttcgtgatt catccaatat tcgttcagat ggtttttggt
     1021 caaacacatg tacagaactg aacagaagta ccttttgtac accgcattct tctgcagctt
     1081 cgagcacatt tttcgcaccg gttacgttca cattataaag tgactggctg aaacgataac
     1141 caagctcgat gttagctgcc gtatgaataa ccacattaca ttcatccatc aatctaatca
     1201 gtgaatcctt atcaagcaca ttaccagaac aaactttcaa agggagacca ttaattgcag
     1261 ggcagtcatt gcgtacaagt gcgtttacct cataaccctg acggacaagt tcgcggcata
     1321 atgctgaccc aatttgaccc gaggctccga ttacagctac tttccaggac attttcaatt
     1381 tatggatgag gaatttcttt tcacaaattt cagtaaatat ccgaaaaata ttcaatccta
     1441 taccgctgca tgcaaaaggt tgaatcaact cttcttggaa ataatttttc tatttccctg
     1501 ccatcagcat tatggttact taactaactt ccacccattc ctcgatgtca aatatcgtga
     1561 aaaactacaa tacaatcagg gtaactgcca caaataccag ataccctgac aatttaaaaa
     1621 accatcggtt tacttaagat tatgccttct catccgcact tggcccatac aaacccggaa
     1681 tcggtatatc aagcaggcga agataaacac ttaactgagc acggtgatgg tagaggtgat
     1741 taagcgccat acttctaata gcaacagcct tgggcaactg aagaatcaat ctgtctcctg
     1801 cccttagtgt ccatatctca ttgagatcct catcattaac cgattgaagc gtctctatgg
     1861 acttttgtgt tctttgttcc aattccgcca ccaaatcgtc atttgttttg acatcaatat
     1921 ttttcaaagt atcacctgcc agatccaatt catccatttt ggcaattagt tcaggccagt
     1981 ctgccaaacc aactatgtgt gtagcaagtg ttttcaattt catggattta gcatgtggcc
     2041 tccattcaaa actttctgca ggcacggctt gtatcatcct tttagttcct tccgattcat
     2101 gcttgatttc ggcaataaga gatttagtaa gtgacataaa ttatttttta ttatgattaa
     2161 aactaatttt tacagcattt aaagcaggtg aatttatttt cttcttaatc ctgatatatt
     2221 acacttaaag acaacaaatc catagcagtt gttgaatgct gcattaatca atgtaaagca
     2281 tcgacctctt agtgccaaac agcgaatcct taataaagtt ttatcttggt gcaaaaaaag
     2341 ccttcttcgg agagaagaag gcctgtttca ataaacttat gttttatttt ttaaattctt
     2401 atcgaagata atttttttat attaattctt gattacttgt aaaggtacac agcgtttaag
     2461 cggtgctgtg taattctttg agtcaacaca atcaggattt ccggtgaagt aagccgtaac
     2521 agtgtgcact gctccgtcag ccggcaaatc aggtatcaca taagcataag ggcttgttac
     2581 aggagccggc acaatgatag tatgtggccc tactttgata acaagatctc ctgatgcagg
     2641 tggagtctgg aatgtaacca caccggtcaa tgaatatttt ccttccggtg aagcacaatt
     2701 ttcagcaatg gctgtaacag ttacacatgg taaacagttg ccaggcttga ttacgacagg
     2761 acaacaaagc tttacttcac atccgttggc gtctgttgct ttccagatat atgtaccgat
     2821 ggtcgttgtt gtataagtaa ggtttgtagc tccgctgatg gcggttgtgt ctgttccgct
     2881 tatcgtgtac cactgatagt tgctcagtcc tgcctgcgca gtaagcgtat aactctcacc
     2941 agggcatatc tcgtattgag ttttatcaca ttcgcaagct actggtgctg tataggtcgc
     3001 actacccgca caattagggt cagcactgaa taccgcagtt acagtatggc tcaatccgtc
     3061 tgacacaagg ccgctcagtg tataactgat agggctcgca aacggtgcgg tgaatgtctg
     3121 gctcttcgta ccactcgtta cagtcaacgt tccggttgat ggcggattgg tgaatgtaac
     3181 agtgccactt acagagtatt cattcgtcaa tggattacat gcaccggcaa tcgctgttac
     3241 cgggcccatt gcacatggta tgatacagtt gccttccttg attacgacag gacaacaaag
     3301 ctttacttca catccgttgg cgtctgttgc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nccgctgatg gcggttgtgt ctgttccgct tatcgtgtac cactgatagt
     3481 tgctcaaacc tgcctgcgca gtaagcgtat aactctctcc agggcatatc tcgtacggcg
     3541 tatctgggca aggcatacaa gctgtgccag ggacaatttt cacaggacaa cacaacgaaa
     3601 catcacaacc attcacatct tttgctttcc aaatgtaagt tccggcattt gacgtgatat
     3661 atgaaatatt cgtttctccg gcaataacga gtgtatcaga tccaacaata gtgtaccatt
     3721 catagctcgc catacctgcc aaagcagtta aagtgtagct ttcgcccgga caaatctggt
     3781 aggtcgtatc aggacacatt gcacatgaag tggaagcatt aatctggaca ggtgtgcaga
     3841 gctttacaag gcaattattt gcgtcctttg cagtccaaag atatgtacct ggggcatttg
     3901 ctatgtagct aatattcgtc gcacccggta tcatagtctc cgtagtgcct gacaatgtaa
     3961 accattgata atcactcaaa ccggtttgtg ctacaagcgt ataagtctca tccggacaaa
     4021 tttgatagac agtatccgga gggagcaggc aagttcctgt tacaatcaag actggttcac
     4081 acagactgac aatgcatccg ctattattgt tggctttcca gatataagta cccggagctg
     4141 atgtagtaaa actcatgttt gctgcacccg gtattgcggt agtgtcaatc cctgaaatac
     4201 tataccactg atagttattc aggccggatt gtgcggttaa tgtatagctt tcgccagggc
     4261 aaataggata atatacctga gaattggctt taaattgaac caaaagcaca agcaggagta
     4321 tccctaccca cttcattgct acttgattta tgaatgaaat atttttcatt atatcataat
     4381 tatgataaag tgtgatcaaa cttttactaa ccaataaaac atcaattcac atcaatgatt
     4441 ttgctgttta gttttctggt attacctgat tccagtttaa attaatttta aaatctgagt
     4501 catcagacaa caggtttatg ggaatctgag aattactctg aacacaaaga actgctcatg
     4561 aatcagaata attcttcatc ctaaaacaag aagtaaccaa aagttgtcgg cacactccat
     4621 cctttattca aaaacagtcg gcttactact aagccaattg aaatgccaat tcttaatttt
     4681 tctgaacagt cactacattg ctgcagtatg gaacgcaagt aacattaagt gtataaacac
     4741 ccacctggca agatgctggt gtagagctgt cacatacttt cacgcaatag taatatttac
     4801 ctacaactgg aggtgcagta taagtataaa cacctgttgt gctgttgatt gaaaaattac
     4861 ttgcaccagg aagagctgta gcacccgaag gggcaacaca accaggctca ccactaccat
     4921 tgctgtaagt gtaaggacca ttaccaccct gagggttcaa ttcagtagcc gagtttccag
     4981 attttggttg attaaccaaa gcattctgaa ccggaggatt ttgaattgtc attccgattg
     5041 caggacatgc attagcgcca gcaccgtagt tacctaaata atcatctcca cctgcaccaa
     5101 aaatagtgat agcattacca ggattggtgt ttgctgagtt agcataatcc ggtggtgtaa
     5161 ggttactgaa tgggtctgaa aagttttcaa aaagttgaag tgcaccaagg caattaccac
     5221 cattatcaaa gctaaatagt ttgatttctg tattggctgg gatgttgaag ctggtagcac
     5281 cctggaagcc
//
LOCUS       DAOQQY010000207         5259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7095_length_5259_cov_5.831860, whole genome shotgun sequence.
ACCESSION   DAOQQY010000207 DAOQQY010000000
VERSION     DAOQQY010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5259
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7095_length_5259_cov_5.831860"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLE23_09600"
     CDS             <1..394
                     /locus_tag="PLE23_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HMX83558.1"
                     /translation="GGVSFIRAMSALDKVKGENEDQNIGVAIVKKALETPLRVIAENA
                     GLEGSVVLNRVKNSRGSHGFNARTEKYEDLKAAGVIDPMKVARVALENAASIAGMILT
                     TECVISEKKEAEAPHSHMPPGGGMGGMM"
     gene            complement(682..2925)
                     /locus_tag="PLE23_09605"
     CDS             complement(682..2925)
                     /locus_tag="PLE23_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX83559.1"
                     /translation="MKYFIGSIMLLCTVISGYSQTLKGTLQSYSGDKIDHAEIKWLGD
                     VIGTYTEEDGTYELERSKVSDKLLISAIGFYSDTVEVEAGLTQFDIKMTPIRQLTEVT
                     VSSKRQDSYVSTLQTKNVETLTAHELKNAPCCSLSESFETNATVDVVQTDAVTGTKEI
                     QMLGLKGIYTQVLLENKPDFGGIGSAYAIDFVPGTWIDAIQISKGASTVVNGYQSITG
                     QINVELIKPNKGKPLYINAYGNHEGRYEANVVLNKKYKSPWSQGLLMHASGQQNRSDH
                     DHDSFIENVLRSTYSGVYRLFYTGDIWDVQLNFTGAYDDRKSGQYAHKHILDDSEGLY
                     HINQTNKRFEVFGKAAFLGMKGEKESFGFQWHAIHHDLNNFIGYRSLLANETDLLANL
                     LYKNRIGNNNNTIEFGLNGQIDNIKQNYENMPLNYNDRTAGLHAEYAYSNKDAHSTAS
                     AFMKNFGIVAGIRLDNHSQQGWFVVPRVNVKYNFTDETILRFSAGRGLRRALPFTDYI
                     NRMANNRTWVVQANLPLEDAWNIGGNFTQSFTLKERNGQIALDAYQVLFNQQVIIDMD
                     ESAKNVYIYALDGKSQSTTIQVSGQYEIFAGMNLKAAYKYSGVKLDYLEGYRDAVFVP
                     KDRWLFTAAYKTPNQKWMFNLLANYTGKMRLPDTDGIPESVLGLNKEESNPFWRFDFQ
                     TTYFAKNFEIYSGIENFMDYRQKSPILDHHNPFGEYFDAGRVYAPLNGRMFSLGVRYW
                     LDSPQTK"
     gene            complement(2982..3368)
                     /locus_tag="PLE23_09610"
     CDS             complement(2982..3368)
                     /locus_tag="PLE23_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83560.1"
                     /translation="MLTTNVVVSIDSIYCYCFKTTTFQITGFEEEECAPESEMACTDN
                     TCCSSSIEKLPCKETKQIIVANQEDFTSNISQLQLKKSFDSFVIPRHTVILPIIDADH
                     FAYANTFNFQPPVTKHSRAFLQIFLC"
     gene            complement(3582..4019)
                     /locus_tag="PLE23_09615"
     CDS             complement(3582..4019)
                     /locus_tag="PLE23_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83561.1"
                     /translation="MKKLFVLTLIITSFVACKQERKELIAKKWVYDTDEMYKVTSKNL
                     EKLEARDKVLEGYKTAYLDRYRDVSYTLDKTGKLVWQDKGVIRNGQWGLADNDSTLIL
                     DADTVQTSFKILKLTSSQMIFQDNVVNTSPQFELSKPILKSVK"
     gene            4112..4726
                     /locus_tag="PLE23_09620"
     CDS             4112..4726
                     /locus_tag="PLE23_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HMX83562.1"
                     /translation="MKTIINFKSVVLLCLTALFFYTTSSAQTSFNPKVGLHISAIDGK
                     FKDTSSLNSRVGWQVGLDIRKGSELFFLNPGLHYIVTSVDLKKEIKTNGGIQDITGST
                     TIQSISLPINGGIYLTGKDQALMRIYIHGGILPNYIIGIKSVEKFDLNPSEFNRFQFG
                     VNGGVGIDLLFLHIGAKYEYYLNDYFKNQPGKNRTISVSLGFVF"
     gene            4791..>5259
                     /locus_tag="PLE23_09625"
     CDS             4791..>5259
                     /locus_tag="PLE23_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMX83563.1"
                     /translation="MIYNYLGKSGVQVSALSLGSWVTFGNQISDKTAAELMHKAYDHG
                     VNFFDNAEIYANGKSEIVMGNILKASGWDRSSFLVSSKVYFGDGNKKPNQTGLSRKHI
                     REACNAALKRLQAEYLDLYFCHRPDRNVPMEEIVFTMTHLIEQGKILYWGTSEW"
BASE COUNT         1543 a         1007 c         1007 g         1702 t
ORIGIN      
        1 cggtggtgta agcttcatca gagcaatgag tgcactcgat aaggtgaaag gcgaaaacga
       61 agatcagaac ataggtgtgg ctatcgttaa gaaagcactc gaaactcctc ttcgtgtcat
      121 tgcagaaaat gcgggtcttg aaggcagcgt cgtcttgaac agagttaaaa acagcagagg
      181 gtcgcacgga tttaatgcaa gaactgaaaa atacgaagac ctgaaagccg caggtgttat
      241 tgacccaatg aaagtagcca gggttgctct tgaaaatgct gcatccattg caggtatgat
      301 tcttactacc gaatgtgtca taagcgaaaa gaaagaagca gaagctccgc atagccacat
      361 gcctccaggt ggtggtatgg gcggaatgat gtaattctga aaatattatt cttaaagtat
      421 gaaggcatca gctcacaggg ctggtgcttt tttttgattg aagaaaaacc gtattctcct
      481 gagattcagc tggtgtatgt taaacccgga ttatgcatca gactctttat tgcaaataat
      541 tattacttca aatccatttc aaaacatttt cgtttaaatt cctaatgcgt tatctgggtt
      601 aataaatcag gtattcgtaa cattgcaaaa aagccctgct ttggattaag cagggcttta
      661 aattaattat gaagtcaata tttattttgt ctgtggagaa tccagccagt atctaacgcc
      721 aagactaaac atacgtccgt taagtggtgc atacactcga cctgcatcaa agtattcgcc
      781 aaaaggattg tgatgatcaa gtattggcga cttttgtctg taatccataa agttctctat
      841 acctgaatat atttcaaaat tctttgcaaa atacgtggtc tggaaatcga acctccagaa
      901 tggatttgat tcttctttat ttaagccaag aacactttca gggatgccat ctgtatcagg
      961 taatcgcatc ttgcctgtat aattagcaag taggttgaac atccatttct gattcggagt
     1021 cttgtaggct gcagtaaata accatctgtc ttttggtaca aaaactgcat ctcggtatcc
     1081 ttccaaataa tcaagcttaa cgcccgaata tttataagca gccttcaagt tcataccggc
     1141 aaatatttcg tattgacccg acacctgaat ggtggttgac tgtgattttc cgtccaaagc
     1201 gtagatgtac acattttttg cactttcatc catgtcaata atcacctgtt ggttaaacaa
     1261 tacctgatat gcatccaatg ctatctgacc gtttcgttcc ttcaatgtaa aactttgagt
     1321 gaaatttcca cctatgttcc atgcgtcttc aagtggaagg ttagcttgta cgacccatgt
     1381 cctgttgttt gccatcctgt taatataatc ggtaaaaggc aatgcacgtc tgagtcctct
     1441 gccagccgaa aaccgcagaa tggtttcatc tgtaaagtta tatttcacat tgactcttgg
     1501 tactacaaac caaccttgct gagaatgatt gtcaagtcgg atacctgcca caattccgaa
     1561 atttttcata aacgcagaag ccgtactgtg tgcatctttg ttagaataag cgtactctgc
     1621 atgcaatccg gcggtacggt cattgtagtt gagaggcata ttttcatagt tttgcttgat
     1681 gttgtcaatc tgcccgttca atccaaattc tatagtatta ttgttgttac caatacggtt
     1741 tttgtataat aaatttgcca ggaggtcggt ttcattagca aggagcgaac ggtaaccaat
     1801 aaaattattt agatcatgat gtatggcgtg ccattgaaat ccaaatgatt ctttctcacc
     1861 tttcatacca agaaaagcag ctttaccaaa aacttcaaat cttttatttg tttgattgat
     1921 gtggtacaaa ccttctgaat catcgagaat gtgtttatgt gcatactggc ctgattttct
     1981 gtcatcgtat gctccggtaa aatttaactg tacatcccat atatcacccg tatagaataa
     2041 cctgtacaca ccgctgtagg tagaacgaag tacattttct ataaaactgt catggtcgtg
     2101 gtccgatctg ttttgctgcc cggaagcatg catcaacaaa ccctgactcc aaggcgattt
     2161 atatttttta ttcagcacga catttgcttc ataccttcct tcatggttac cgtatgcatt
     2221 gatgtacaat ggttttcctt tgtttggttt aattagttca acgttaattt gtccggttat
     2281 tgattggtat ccgtttacta ctgttgaagc tccttttgaa atctgaattg cgtcaatcca
     2341 cgtacctgga acaaaatcaa tggcataggc actaccgata ccaccaaaat ccggtttgtt
     2401 ttctagcaat acctgtgtat aaatcccttt cagtccgagc atctgaattt cctttgtgcc
     2461 ggtcactgcg tctgtttgta cgacatcaac tgtggcattg gtctcaaagc tttctgaaag
     2521 gctacagcaa ggagcatttt tcagttcatg tgctgtcagc gtttcgacgt ttttggtttg
     2581 gagtgtgctc acataactat cttgtctttt tgaagagact gtcacttccg tcaattgtct
     2641 gataggcgtc atcttgatat caaactgtgt caaacctgct tcgacttcga ctgtgtcgct
     2701 ataaaatcca atagcagaaa tgagaagctt gtccgatacc tttgagcgtt ctagttcata
     2761 ggtgccgtct tcttcggtgt atgtccctat cacatcaccg agccatttta tctcagcatg
     2821 atcgatttta tcacctgaat aagattgaag agttcctttt aaggtttgcg aatatcctga
     2881 tataactgta cataacagca tgatgctgcc aataaaatat ttcataatgt taattttaaa
     2941 aatttgaaaa aatagataca aaaaattaaa acaacattat tctaacacag gaatatctgt
     3001 agaaacgccc tggagtgttt agttacgggc ggttggaaat taaaagtatt tgcataggca
     3061 aaatgatcgg catcaatgat aggtaaaatg accgtatgcc tcggaataac aaaagaatcg
     3121 aaagattttt ttagttgtaa ttgtgaaatg ttgctggtaa aatcttcctg attggctaca
     3181 attatttgct tggtttcttt gcatggaagc ttttctattg aagaactaca gcaagtattg
     3241 tccgtacatg ccatttccga ctcaggggca cattcttcct cttcaaaacc ggtaatctgg
     3301 aatgtggtag ttttgaaaca atagcaataa atagaatcaa tacttacgac cacgttggtg
     3361 gtaagcatca acagcatcaa aattgcactt aatattttct ttctcaattc cacgctacaa
     3421 aattgcgaca ttttgaatat aaatccaatc aaacggccta gtgttaagtt aattttaaca
     3481 tgttctgaac gaattttaag acaaatttat aatgaacaca actgaattag gaaattatga
     3541 attctccttt cttgaaaatt gtgacccatt gcagattcca gctattttac agactttaga
     3601 attggcttgc tcaactcaaa ctgtggactt gtgttaacaa cattatcctg aaaaatcatc
     3661 tggctagacg tcaatttaag gattttgaag gaagtttgaa cagtatctgc gtcaagtatc
     3721 agtgtgctgt cattatctgc gagaccccat tggccatttc ttatgacacc tttgtcttgc
     3781 cacacaagtt tacctgtttt atcaagcgta tatgaaacat cacgataacg gtcaaggtat
     3841 gctgtcttgt acccttctag taccttgtca cgtgcttcaa gtttttcaag atttttgctg
     3901 gtcaccttgt acatttcatc ggtatcatat acccattttt tggcaataag ttcttttctt
     3961 tcctgtttac aggccacaaa tgaggtaata atcagtgtta atacaaataa ttttttcata
     4021 aaattttctt aatatcttgt tttaaagtat gcaaaatcaa ggtctcaaac gttatttggt
     4081 taattttacg tcagctttca aatttggttt aatgaaaaca ataataaatt ttaaatccgt
     4141 agtgttactt tgtttaacgg cattgttctt ttacactact tcttcggctc agacaagctt
     4201 taatcctaaa gtcgggttgc acatttctgc tatcgacggc aagtttaaag atacctcgtc
     4261 gcttaattcg agagttggct ggcaggtagg tttggatatt cgaaaaggga gcgaattgtt
     4321 tttcctaaat cctggtctgc attacattgt cacctcggtt gaccttaaaa aagaaataaa
     4381 gacaaatggt ggcatccagg atatcactgg ttctactacc atccaatcca tcagtttgcc
     4441 cattaatggt ggaatatatc ttaccggaaa ggatcaggcc ttgatgcgta tatatattca
     4501 tggcggtatc ttacccaatt acatcattgg aatcaagtcc gttgaaaaat ttgatctcaa
     4561 cccttccgaa tttaatcgtt ttcaatttgg tgtaaacgga ggtgtaggta ttgatttgct
     4621 tttcctgcat ataggtgcca agtatgaata ctacttaaat gattatttta aaaatcagcc
     4681 gggtaagaac agaactattt ctgttagtct gggttttgta ttttaacagg tttatccctt
     4741 taaggtgatt tactctgtac gaactatttc tttcatcaaa agcgaaaatt ttgatttata
     4801 attatttagg taaatctggt gtgcaggtta gtgcactttc acttggctca tgggtaacat
     4861 ttggcaatca gataagtgac aaaacagccg cagaacttat gcataaggca tacgatcatg
     4921 gtgtcaattt ttttgataat gccgaaatat atgccaatgg taagtcggag atagtaatgg
     4981 gcaatattct aaaagcatcc ggctgggacc gttcatcatt tttggtaagc agtaaagtat
     5041 attttggtga tggcaacaaa aagccaaatc agactggtct gagtagaaaa cacataagag
     5101 aagcgtgcaa tgccgcactc aaaagacttc aggcagagta tctcgacttg tatttttgcc
     5161 ataggccaga tcgcaacgta cctatggaag aaattgtttt caccatgacg cacctcatcg
     5221 agcaaggtaa aatactttac tggggcactt cggaatggt
//
LOCUS       DAOQQY010000208         5138 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7370_length_5138_cov_6.981114, whole genome shotgun sequence.
ACCESSION   DAOQQY010000208 DAOQQY010000000
VERSION     DAOQQY010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7370_length_5138_cov_6.981114"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PLE23_09630"
     CDS             complement(<1..1190)
                     /locus_tag="PLE23_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83564.1"
                     /translation="MKKTLFVITAILVCLTGISQPTSFAPRGMGGGGAMFFPSINPYN
                     EDEYYVASDLSAMFHSTDYGKSYDQVDFQKLQVGGNSTYEFTNDPMIAYCNFNDGNDG
                     YPVKTSDGGVTWSRLMAYDVENYGGIYSMKANIYNVSQLLVSAYGDILFSNNGGASFS
                     TVKSADDNGAGLIMGGVFWDGSNIYIGTNDGLIVSNNGGNSFTVMPTTGIPANERIWS
                     FAAAKEGGITKFVCITADVADVYNGVAPWDYYDFAKGVYVMDNVSGLWQSVSTGIDFS
                     ADFIMYAAMAYNDIDRIYLGGADDNTGGPLVFKSENGGSTWAKVFNTVNNANIITGWE
                     GHGGDKTWGWSENCFGLTVAPFNSARVMFGNFSNVQLTSDGGLTWRQAYVGQADENPA
                     GQATP"
     gene            1575..2243
                     /locus_tag="PLE23_09635"
     CDS             1575..2243
                     /locus_tag="PLE23_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83565.1"
                     /translation="MKSSVKNWSLSQIIRSTGIFALLLVALFSMQSCSKEETSPLETP
                     AYTSLNSAMRKLWSDHASWTREVIVNFATNAPGLNESINRLLKNQEDIGNAIKPYYGN
                     EAGDALTTLLKEHITTAAEILTAAKAGNTTAQNDAAARWYANGDQIATFLSTANPKNW
                     PLDHMKSHMKDHLDLTLEEAVAELTGNYTASVAAYDKIYPQLLHLADLLSEGVALQFP
                     DKFK"
     gene            2661..3308
                     /locus_tag="PLE23_09640"
     CDS             2661..3308
                     /locus_tag="PLE23_09640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMX83566.1"
                     /translation="MNVLEKFTDADVISKVLNGEKAMYEIIVRRYNPYLYKIGRSYNY
                     SHDDTQDLMQDTYIDAYKSLDKYEGRSNFKTWISRIMLNNCFRKKEKSSFKKEVMEEF
                     NEHSTPLFAAAENKTATIVHSRELGTIIEQALDNIPLDYKMVFTLREMNQMSVAETSE
                     LLQISEANVKVRLNRAKEMLRQHIEKSYTATELYEFNLVYCNAMVDRVMNIIDKL"
     gene            3324..3962
                     /gene="folE"
                     /locus_tag="PLE23_09645"
     CDS             3324..3962
                     /gene="folE"
                     /locus_tag="PLE23_09645"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HMX83567.1"
                     /translation="MQKTMLNNKTSPQSVSPIKAIANQLSESEKISKIQYHFAEIMDA
                     LGLDLTDDSLKDTPKRVAKMFVKESFSGLDPKNKPSISLFENKYGYHRMLVEKDITLY
                     SNCEHHFVPIIGKVHVAYIPGDKVIGLSKINRLVQYYGKRPQVQERLTMQIAEAMKEI
                     LQHDDVAVVIEADHLCVASRGIHDTNSMTTTSCYMGQFENKALQQEFLTYIK"
     gene            3972..4640
                     /locus_tag="PLE23_09650"
     CDS             3972..4640
                     /locus_tag="PLE23_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMX83568.1"
                     /translation="MAQAVKIKSIEHLTHDVLKIVADKPEGLSYTPGQAVDVSVNKPG
                     WEEELRAFTFTSQPEDDFIEFNIKTYPSHNGVTNQLQSMQAGDELLIGDVFGDIHYKG
                     EGVFIAGGAGITPFIAIINQLEKENKIGNNKLLFANKSTKDIFNKERFERQLGSHYIN
                     VLSDEKTEAHEHGYITADIIKKYAGDNTKYFYLCGPPPMMDAVEKSLASLGITEESIV
                     KEPF"
     gene            complement(4774..>5138)
                     /locus_tag="PLE23_09655"
     CDS             complement(4774..>5138)
                     /locus_tag="PLE23_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HMX83569.1"
                     /translation="VLPIGGHGIFIDAKRFLPHLTQDQFPAQALAAELYIDSGVRTME
                     RGIVSAGRNAQTGDHYYPKLELVRLTMPRRVYTQAHMDVIAESVANVYKNRKKITGLK
                     MVYEPRYLRFFQAQFEKL"
BASE COUNT         1702 a         1165 c          929 g         1342 t
ORIGIN      
        1 ggtgttgctt gcccggctgg gttctcgtcc gcttgcccga cataagcctg cctccatgtg
       61 agtccaccgt ctgaagtcag ctgtacattg ctgaagttac cgaacatcac tctcgcagaa
      121 ttaaacggtg ctactgtcag tccgaagcag ttctcgctcc agccccatgt cttgtcgccg
      181 ccatgcccct cccagcctgt gataatgttg gcattattga ccgtattgaa gacctttgcc
      241 caggtgcttc ctccattttc tgacttaaaa accaatggcc caccagtatt gtcatctgcc
      301 cctcccaaat atatacggtc aatatcgtta tatgccatgg cagcatacat aatgaagtcg
      361 gcactaaaat ctatgcccgt tgaaacagat tgccataagc cggatacatt gtccatcaca
      421 tacactcctt tggcgaaatc ataatagtcc cacggtgcta caccattgta cacatcggct
      481 acatcagcag ttatacacac aaacttagta ataccgcctt cctttgcagc agcaaaactc
      541 caaatacgct cattggcagg aattccggtg gtgggcatca ctgtaaagga gttaccacca
      601 ttgttcgaca caataagacc atcattggta ccaatgtata tgttgcttcc gtcccagaat
      661 acgcctccca tgatcagtcc ggcaccattg tcatcagcgc ttttcaccgt actgaagcta
      721 gcacctccat tattcgagaa aagaatatcg ccataagcac ttactaataa ttgcgaaaca
      781 ttataaatat ttgccttcat ggaatagatc cctccataat tttcaacatc ataagccatg
      841 agccgactcc aggttactcc accatcggat gttttaacag gatagccgtc attgccatca
      901 ttaaaattac agtatgctat catcggatca tttgtaaatt cgtaagtaga attgccgcct
      961 acctgaagct tctgaaaatc tacctggtcg tatgacttcc cataatcggt actgtgaaac
     1021 atggccgaca aatcggaggc tacataatac tcatcttcat tgtaagggtt gatgcttgga
     1081 aaaaacatgg caccaccgcc acccatccca cgcggagcaa aggaagtagg ttggctgata
     1141 cctgtcaagc atactaaaat agccgttatg acaaacaagg tctttttcat tttttcatag
     1201 tttttggtgg gttggtcaaa tatatacaat agactctttt tccaccagat tcgttactgc
     1261 aatttttttt gcaacagaga aaattcttac ctgtaagcaa tattccgttg atagcccgga
     1321 ctcattttct cccttaacag tagctcacaa taatcattcg aaagataaat taatccaaaa
     1381 tatcgcaaaa ggcgaaaatc cttaaaataa attaactgag ctatatcatt gtatatcaat
     1441 acattagaaa ataatcattc aacaataaaa aaaaataatt caatcaaact actgattttc
     1501 tgtaacctca cacaaagaca tttactctat aagatcaaac agcttattct ttaaacattt
     1561 aaaaaatcaa caaaatgaaa tcatccgtta aaaactggtc tttgagccag atcatccgtt
     1621 caacaggaat tttcgcactt cttttagtgg ctttattcag catgcaatct tgctcaaaag
     1681 aagaaacctc accactcgaa acaccagcct acacttcact taactcagcg atgagaaaac
     1741 tatggtcaga ccatgcatcc tggacacgtg aagtaatcgt aaactttgca accaatgcac
     1801 ccggcctgaa cgaatctatc aaccgattgc tgaaaaacca ggaagacata ggtaatgcaa
     1861 tcaaaccata ctacggaaac gaagcaggtg atgcccttac taccttgtta aaagaacaca
     1921 tcaccacggc tgccgaaatc cttacagctg ccaaagcagg caatacaact gcccagaacg
     1981 atgccgctgc cagatggtat gccaacggtg accagatagc cactttcctc agcactgcca
     2041 atcctaaaaa ctggccactt gaccacatga aatcacacat gaaagaccac cttgatctaa
     2101 cgcttgaaga agcagtagcc gaacttacag gcaactacac agcaagcgta gcagcttatg
     2161 ataagatata cccacagctt ctacatctgg ccgatctgct ttctgaaggt gtagccctac
     2221 agtttcccga caaatttaaa taaataaaca ctaaaaccgc tcaccgcctt gtctgtaaaa
     2281 gtttacttgc gcagacgggg cggtatttaa gccaaaaacc ggctatgccc ccgatttccc
     2341 tctcctactt tgattttcca tcatttgcct ttctttgaag aaaatctata ttcttccgtt
     2401 atgttgtttt atatgatttt acttttttaa aaaaaatcag atattatttg taacctattt
     2461 tacattgtgt tactccaatg gtttaaatgg ttttatcaat gatgtattgt ttttccttca
     2521 attattaaac caaaaagaaa aaggttcatt ttaataagaa gagaatttaa acacgcacca
     2581 aaaaaaccaa tatttgaaaa gtaatattta tgactaaagg cctttggcaa attgttgaaa
     2641 ttatataaaa aatagaatgt atgaatgtac tggaaaaatt taccgatgca gatgtaatta
     2701 gcaaggtatt gaatggtgaa aaggcaatgt atgaaataat tgtaagacgg tacaacccct
     2761 acctgtacaa aataggaagg agttacaact acagtcatga tgatacccaa gatctcatgc
     2821 aagacactta tatagatgca tacaaaagcc tcgacaaata cgaaggccgg tccaatttca
     2881 agacgtggat tagccgtatt atgctcaata attgtttccg aaaaaaggaa aaatcaagtt
     2941 ttaaaaaaga agtcatggaa gaatttaatg aacactcgac cccattgttt gctgctgctg
     3001 aaaacaaaac agcaaccatc gtacacagca gagaactggg tactatcata gaacaagctc
     3061 ttgacaatat cccattggat tataagatgg tgtttacact cagagagatg aaccaaatga
     3121 gtgtagcaga aacatcagaa ctcctccaga ttagtgaagc caacgtaaaa gtacgactca
     3181 atcgggcgaa agaaatgctc cgacaacata tcgaaaagtc atacactgcc accgaactat
     3241 atgaattcaa cctggtgtat tgtaatgcaa tggttgaccg tgtaatgaac atcattgata
     3301 aactctaaga aaaaactaag gaaatgcaaa agacgatgct aaataataaa acttccccac
     3361 agtcagtttc gccgataaaa gcgattgcta atcaattatc agaatcagaa aaaataagca
     3421 agattcaata tcattttgcc gaaataatgg atgcactcgg cctggacctt acagacgatt
     3481 ctctgaaaga tacgccgaag agagtagcaa aaatgttcgt aaaagaatca ttcagtggcc
     3541 tcgacccaaa aaacaaaccg tctatcagtt tgtttgaaaa taagtacgga taccaccgca
     3601 tgctggtaga aaaagacata acgctttatt ccaactgtga acaccacttt gtgcccatca
     3661 tcggcaaggt tcacgtggct tacatacccg gcgataaagt gatcgggctt tcgaaaataa
     3721 accgtctggt acagtattac ggcaaacgac cacaggtaca ggaacgcctg accatgcaga
     3781 tagcagaagc aatgaaagaa atactacaac acgatgatgt agctgtcgtc attgaagctg
     3841 atcacctttg tgtagcatca aggggcattc atgacaccaa cagcatgact actacttcat
     3901 gttatatggg tcagtttgaa aacaaagcgc tacaacagga atttttaaca tacataaaat
     3961 agtacaaaat catggcacaa gcagtaaaaa taaaatcgat agagcacctg acacatgatg
     4021 ttctgaaaat agtagccgac aaacctgaag gcctgagtta cactccaggg caggcagtag
     4081 atgtctctgt caataaaccg ggatgggaag aagaactacg ggcattcacc tttacttcac
     4141 aacctgaaga tgattttatt gaattcaaca tcaagactta tccgtcacac aatggtgtta
     4201 ccaatcagct gcagtctatg caggccggcg acgaactctt gataggcgat gtttttggag
     4261 atatccacta caaaggcgaa ggtgttttta tagcaggtgg tgcaggtatt actccgttta
     4321 ttgccatcat taaccagctt gagaaggaaa acaaaatcgg aaacaacaaa cttctgtttg
     4381 ccaacaaatc aaccaaagat atttttaaca aagaacgctt cgaacgacaa cttggcagcc
     4441 attacatcaa tgtgctttct gacgaaaaaa ccgaagcaca cgaacacggt tatattacag
     4501 ccgatatcat caagaaatac gccggcgaca acacaaaata cttctacctg tgcggaccac
     4561 cgcctatgat ggatgccgta gagaaaagcc tggcaagcct gggtatcaca gaagaatcaa
     4621 tagtcaaaga accattctga cgaaaaaaca gagtaagtaa attcttacaa accttaatca
     4681 cttttataac cctaatattt tcataaaaac agaccgacat acggatcaaa ttgattcgct
     4741 gtcggtcttt tatattatgt ctgttgttat caattacaac ttttcgaact gagcctgaaa
     4801 aaacctaagg tatcttggtt cgtaaaccat cttcaatcct gtgattttct tcctgttttt
     4861 atacacattt gccacagatt ctgctatgac gtccatgtgt gcctgggtat atacacgcct
     4921 tggcatagta agccttacga gctcaagctt cggatagtaa tggtcgccgg tctgtgcatt
     4981 cctgccggct gaaacgatgc ctcgttccat agtcctcacg cctgaatcaa tatacaactc
     5041 ggcagccagt gcctgtgccg ggaactggtc ttgggtaagg tgtggcaaaa agcgttttgc
     5101 atcaatgaaa ataccgtgtc cgcctattgg cagcacaa
//
LOCUS       DAOQQY010000209         5130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7389_length_5130_cov_6.743645, whole genome shotgun sequence.
ACCESSION   DAOQQY010000209 DAOQQY010000000
VERSION     DAOQQY010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7389_length_5130_cov_6.743645"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2529)
                     /locus_tag="PLE23_09660"
     CDS             complement(<1..2529)
                     /locus_tag="PLE23_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HMX83570.1"
                     /translation="MNEANNYWHQLQALKETNSEDIQSLYAVFEDLIYKVTDAVNITF
                     STFYVRMSYVFHSYPVPGNIQWQLYRLRRLNLEKNIAEDKKEVPSDDIHKYHNATASL
                     VSVLFKEKIPSESVFTDELPKIFENNNLKFYPSLRAVVQHVDYTKKILHAVSEKLPGE
                     AITIETDAPELYPELFIPNDWLGLPYTISLIQSKVQDNGHFMPELIILEPDYLVDVTT
                     VSEISADAENIGFHLLTKKFITQAVTSSLLEGHAANLFLDNLVKNPDAGFREIFTELF
                     HTYPLVLAKYDDDKLKELYENCKLHYSTLKWVTQKEFPKYNIFSDGIFVEPSFINPVI
                     GIQGRLDILFRGDNNNQSIIELKSGKPFMENKYGLSRSHYYQTLLYDMLVKAVSRHDA
                     TTSCFILYSKLGEQGLRYAPPVKSMQNEALRVRNQIFALEKQISVNGNLENIISDLSL
                     DDKSLKGFLYSNWKYFKNVLNNADDISKTYFYACVAFISREHLTAKTGVDVSDRLTGQ
                     SAVWLETTTRKEDNFSIFKGLTIREVKLSQEEPLVILYKSELTNKLANFRAGDIVILY
                     PGESQTPARNQIFKCTIIELDGDKIVLRLRATQLNSTVFFDHKVWNIEPDMLESSFNG
                     LYRGVFDFLSSSEDKRHKILGLSPPRCSDKMYDPPPYLIDSQRKIYQKALQAEDYFLI
                     WGPPGTGKTSRLVKALTEYYYTSQTKPILIAAYTNRAADEICEAIESINIEIKNHYVR
                     VGARYSTSPAYRQNLLENKLKTCTQRTEVLKVLKDQLIVVGTLASISGKPELFDYYDF
                     ACGIVDEASQVLEPTMAGLMSKLPKTILIGDHKQLPSIVTQPEKL"
     gene            complement(2701..3045)
                     /gene="rplT"
                     /locus_tag="PLE23_09665"
     CDS             complement(2701..3045)
                     /gene="rplT"
                     /locus_tag="PLE23_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HMX83571.1"
                     /translation="MPRSVNHVASRARRKKVLKAARGYFGARSKVFTVAKNAVDKAMG
                     YMYRDRKFKKRAYRRLWIARINAAVRMHGMNYSTFISKLNKSNIDLNRKVLADLALNE
                     PQTFASIVNSVK"
     gene            complement(3127..3312)
                     /gene="rpmI"
                     /locus_tag="PLE23_09670"
     CDS             complement(3127..3312)
                     /gene="rpmI"
                     /locus_tag="PLE23_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HMX83572.1"
                     /translation="MKTNSSAKKRFSLTGSGKVKCSHAYARHLLRKRTKKAKLNLSHS
                     TTVNVADEARIKRLLCV"
     gene            complement(3401..3985)
                     /gene="infC"
                     /locus_tag="PLE23_09675"
     CDS             complement(3401..3985)
                     /gene="infC"
                     /locus_tag="PLE23_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMX83573.1"
                     /translation="MAKKIKEQDLLSQKFNINERIRAFEVRLVGDKLDAISQAAGRKV
                     ELGVYPLHQLIKWAEEMELDVVEISPNANPPVVKITDFQKFLYDKKKREKEIKANTSK
                     TVIKEIRFGPNTDEHDFEFKVRHAKKFLEEGSKVKAYVTFRGRSIVFQDRGELLLLKF
                     LKELEEVGSSEALPKLEGRKMMVMLSPKKTVKKA"
     gene            complement(4086..>5130)
                     /locus_tag="PLE23_09680"
     CDS             complement(4086..>5130)
                     /locus_tag="PLE23_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX83574.1"
                     /translation="TEDDVAEVIAMMTGIPVKRVAQSESNKLVKMADDMKKQIIGQDD
                     AIVKITKAIQRNRVGLKDPKKPIGSFIFLGPTGVGKTELAKALARYLFDSTDTLVRID
                     MSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRRKPYSVILLDEIEKAHPDVYNILL
                     QVLDDGQLTDGLGRKVDFKNTLIIMTSNIGARQLKEFGTGVGFATKARQESEEDDSKA
                     VITSALKKTFSPEFLNRIDDVIVFNSLSQPDIFKIIDISIKELLNRLSHLEYSLELDE
                     KTKEFLSEKGYDPQFGARPLHRAIQKYIEDPLAEFILGENPAPGSVFLASVDESGEKL
                     IISMKKTSKSDVK"
BASE COUNT         1365 a          976 c         1007 g         1782 t
ORIGIN      
        1 aagcttttct ggttgtgtta cgatagatgg caattgtttg tggtctccaa ttaggatggt
       61 cttgggcagt ttgctcatta acccggccat tgtaggttca agcacctgag atgcttcatc
      121 gacaattcca caagcaaaat cgtaataatc aaacagttca ggtttgccgc taatggaagc
      181 cagcgtgcca actacaatca gctggtcttt taataccttg agtacttctg tcctttgggt
      241 gcatgttttg agtttgtttt cgagtaagtt ctgacggtag gccggactgg tgctgtatct
      301 ggctccaact ctcacatagt gatttttaat ctcgatgttt atactttcaa tcgcctcaca
      361 tatttcatcg gctgcccggt ttgtatatgc ggcaataaga atcggtttgg tttggctcgt
      421 gtagtaatat tcagtcaagg ccttgacgag tcttgaagtt tttcctgtac ccggcggtcc
      481 ccaaatgaga aaataatcct ctgcttgtaa tgctttctgg tatattttac gctggctatc
      541 aatcagatag ggtggtggat cgtacatttt atcagaacaa cgaggaggtg ataacccaag
      601 aattttgtgt cttttatctt cagaactgct gaggaaatca aaaacaccac gatacagacc
      661 gttgaaactg ctttcaagca tatcaggttc tatgttccag actttatggt cgaagaaaac
      721 tgtcgagttg agttgtgtgg ctcgaagcct taatactatc ttatctccat ccagttcaat
      781 aatagtacat ttaaatattt ggttccgggc aggtgtttgc gattcacccg gatataatat
      841 tacgatgtct cctgcacgga aattggcaag tttgtttgta agttcggatt tgtagagtat
      901 taccagtggt tcttcttgac tgagttttac ttcacggatg gtaagacctt tgaatattga
      961 aaaattatct tctttacgtg tggttgtttc aagccaaaca gctgattgac cggtgagtct
     1021 gtccgaaaca tcaacacctg tttttgctgt caggtgttca cgcgatatga atgctacaca
     1081 agcatagaaa taagttttac ttatatcatc tgcattgttc aagacatttt tgaaatattt
     1141 ccagttgctg tacagaaaac cttttaaact tttatcatcc aaacttaaat cactgataat
     1201 attttcaaga tttccgttga ctgagatttg cttttctaga gcgaaaatct ggtttcggac
     1261 acgtaatgct tcgttttgca ttgattttac gggtggtgca tatctaaggc cttgttcgcc
     1321 tagttttgaa taaagtataa aacaactcgt agtggcgtcg tgcctgctga ccgccttgac
     1381 gagcatgtca tacagcaggg tctggtaata atgcgatcgg ctaagaccgt atttattttc
     1441 cataaatggt ttgccactct ttaattcaat gattgactgg ttgttattgt cacctctaaa
     1501 taatatgtcc agacgtcctt ggataccaat gaccggattg ataaaacttg gctcaacaaa
     1561 aattccatcc gagaaaatat tatatttagg aaactccttt tgtgtcaccc attttaaggt
     1621 tgaatagtgg agtttacagt tttcatacaa ctccttgagt ttgtcatcat catattttgc
     1681 aagcacaagc ggatatgtat ggaagagctc agtaaatatt tcacgaaaac cggcatcggg
     1741 atttttgaca aggttatcaa ggaaaagatt ggctgcgtgt ccttccaaca gacttgatgt
     1801 tactgcttga gttataaatt ttttggttag aagatgaaaa ccgatatttt ccgcatcagc
     1861 cgatatttcc gatacggtag tgacgtcaac gagatagtcc ggctccagaa tgataagttc
     1921 gggcataaag tgtccgttat cctgtacttt tgactgaatc agtgatatag tgtaaggtaa
     1981 gccaagccag tcgtttggta taaacaattc gggatacaac tctggagcat ctgtttcaat
     2041 tgttatggct tctcctggca atttttcaga aacagcgtgg agtatttttt ttgtataatc
     2101 gacgtgttgc acaacagctc tcagagaagg gtagaatttc aggttattat tttcaaatat
     2161 tttgggtaac tcatctgtaa aaacagattc ggaaggtatt ttttccttaa aaaggacgga
     2221 taccagactt gctgttgcat tgtggtattt atgtatatca tcagatggta cttctttctt
     2281 atcttcggca atattttttt ccagatttaa cctacggagc cggtacaatt gccactggat
     2341 attgcctggt accggatagc tatggaatac ataactcatc cgcacataga atgtagaaaa
     2401 agtaatattt acggcatccg ttactttgta aatcaagtct tcaaatacag catacaggct
     2461 ttggatatct tctgagttgg tttctttaag tgcttgtagc tggtgccagt aattgttcgc
     2521 ttcgttcata attgcttttc tgcttcggat tcatttttaa gacgtaattg tcgaaagaat
     2581 tccataaatt taggaaataa attttcttga aagtgtaatt ttttcaaatg caaatgtatc
     2641 aaaatagaaa aagccacctt atataaggtg gcttccatat ttttgttgta aaatgaacgt
     2701 ttattttaca gagtttacga tgcttgcaaa agtttgtggt tcattcaaag caaggtcagc
     2761 aagtactttt ctgttcaagt caatattact tttgttaagt tttgaaatga aagtagaata
     2821 gttcattcca tgcattctga ctgcggcgtt gatacgagct atccaaaggc gtctgtaggc
     2881 gcgttttttg aattttcggt cacggtacat gtaacccatt gccttatcaa cggcattttt
     2941 ggctacagta aagactttgc ttcttgctcc aaagtaacct ctggcagctt ttaagacttt
     3001 cttccttcta gcgcgggaag cgacgtggtt cactgaacgt ggcataaatt tgatttttta
     3061 gtgcgataca gagattcctt tatacaggaa tactttggcc tgcatcctcg ttaacaaata
     3121 aaattattaa acacaaagta gtcttttgat tcttgcttca tctgctacat taactgtagt
     3181 agaatggctc aaattcaact tagccttctt tgtgcgcttg cgtaaaaggt gacgcgcata
     3241 agcatgcgaa cactttactt ttcctgatcc agtaagagaa aatctctttt ttgcactgga
     3301 gtttgttttc attttaggca taaaattctt gctttttagt aagaggtggc aaagataaat
     3361 actttttcgg agtattacaa gtaaatatct tttaaaaata ttaagctttt tttactgttt
     3421 tcttaggtga tagcatgacc atcatcttac gaccttcgag ttttggtaac gcttcagacg
     3481 aacctacctc ttcaagttct tttagaaact tgagcaataa caactcacct ctgtcctgaa
     3541 acacaattga acgacctctg aatgtaacat aagcttttac ttttgaaccc tcttcgagaa
     3601 atttctttgc atgccttact ttaaactcaa aatcatgctc atctgtatta gggccgaatc
     3661 ggatttcctt aataaccgtc ttggatgtat tggcttttat ctctttttct cttttctttt
     3721 tgtcgtatag aaacttctgg aaatcagtaa tttttacaac aggtggattg gcattgggtg
     3781 aaatttctac aacatccaat tccatttctt ctgcccactt tattaattga tggagtgggt
     3841 acactcctaa ttctactttg cgaccggcag cctgactgat agcatctaat ttgtctccaa
     3901 ccaaacgaac ttcaaaggct ctgatccttt cgttaatgtt gaatttttga cttaataaat
     3961 cctgttcttt tattttttta gccaagtgat tgattttttg atgaaaaata attttagcaa
     4021 atgtacatat tattgcgtga agttaaaaaa ggatttgcac ttctaaatac aaatcctttt
     4081 gaaatttatt taacatctga ttttgaggtc tttttcattg aaattatcag cttttcgcca
     4141 ctttcatcta cactcgccag aaaaacgctt ccgggtgcag gattttcacc aaggataaat
     4201 tctgcgagtg ggtcttcaat atatttttgt atggctctgt gtagcggtcg ggcgccgaac
     4261 tgtgggtcat aacctttttc tgacaagaat tccttggttt tttcatctaa ctcaagtgag
     4321 tattcaagat ggctcaatct gttgagtaat tctttgatgc tgatgtctat gatcttgaaa
     4381 atatcgggtt gtgagagact gttgaatacg atgacgtcat ctattctgtt gaggaattcc
     4441 ggagagaatg tcttcttaag tgcagatgtt atgactgctt ttgaatcatc ttcttctgat
     4501 tcttggcgag ccttagtggc aaaaccgact cctgtgccga attcttttaa ttgtctggca
     4561 ccgatattgg aagtcatgat aatgagtgta ttcttgaaat ctaccttgcg gccgagcccg
     4621 tctgtcaact gaccatcatc aagtacttgt aacagaatgt tgtacacatc gggatgtgcc
     4681 ttttctattt cgtctaacag tattacagag tatggtttac ggcgtacctt ttcggtcaat
     4741 tgtccgcctt cttcgtagcc tacataaccc ggaggtgcac ctatcagacg gctgacagaa
     4801 aatttttcca tatattcact catatctata cgtactagcg tatctgttga gtcaaataag
     4861 tatctggcaa gtgccttggc gagttctgtc ttacctacac cggtagggcc aaggaaaatg
     4921 aatgaaccaa taggtttttt cggatctttg agtcctacac ggttgcgttg tatagccttt
     4981 gttattttga ctattgcatc atcttgtccg atgatttgct tcttcatgtc atctgccatt
     5041 tttacaagct tgttgctctc tgactgagcc actctcttca ccggtatgcc tgtcatcatg
     5101 gctatgacct ctgcaacgtc atcttcagta
//
LOCUS       DAOQQY010000210         5102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7457_length_5102_cov_5.118883, whole genome shotgun sequence.
ACCESSION   DAOQQY010000210 DAOQQY010000000
VERSION     DAOQQY010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5102
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7457_length_5102_cov_5.118883"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLE23_09685"
     CDS             complement(<1..209)
                     /locus_tag="PLE23_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83575.1"
                     /translation="MVPKFVVATARKIITTAYTLTSCSHIRHHEADATNRKRRDSMSG
                     MPNRKTTIGTYHDFSLRTAQLGHAP"
     gene            571..1014
                     /locus_tag="PLE23_09690"
     CDS             571..1014
                     /locus_tag="PLE23_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83576.1"
                     /translation="MKNVIYSLSAILVLFIFDSCKKEDSPKPQNLISGKWQWQQSVGG
                     FSGSAVLTPATEGYTRQFNFGKDSVTITLNDTSFEDRAAFHLTNEKSILYHTTHDFLT
                     IDYHFTIGGDSVVVLPMRYIIKDLKPEKMVLTEDVFDGFEITLAK"
     gene            complement(1085..2950)
                     /gene="mnmG"
                     /locus_tag="PLE23_09695"
     CDS             complement(1085..2950)
                     /gene="mnmG"
                     /locus_tag="PLE23_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMX83577.1"
                     /translation="MFPEYDVIVVGAGHAGCEAAVAAANMGSKVLLITMNMQTIAQMS
                     CNPAMGGVAKGQIVREVDAMGGYSGIVTDHTMIQFRMLNLSKGPAMWSPRAQSDRMAF
                     ALKWRKMLEDNHSIDFWQEMVSSIIVKNGVVKGVRTSMGIEIMSKAVVLTNGTFLNGV
                     IHIGEKRFGGGRAGEKSTTGITEQLTELGFEAGRMKTGTPARIDGRSLDYSAMEVQHG
                     DENPTGFSFTDTPRLENQIPCHITYTNEKVHEILRTGFEFSPMFAGRIQGRGPRYCPS
                     IEDKIDRFADKERHQLFIEPEGRDTCEIYVNGFSSSLPEDVQYKAMTYIPGFKNVKMF
                     RPGYAIEYDYFPPTQLQMSLETRLVKNLFFAGQINGTTGYEEAACQGLIAGVNAHLAI
                     HDLEPLVLARSEAYAGVLIDDLVTKGTEEPYRMFTSRAEYRILLRQDNADIRLTPLLH
                     KLGMHNLDERMARVEEKLKNISLIRKHFTETGVSPDLMNGYLRELGSAPLNQQVKLAS
                     VLSRPMVSMHGMMDAVPALKHYLSAFDDETLNLAEIEMKYEGYIQKEREMAEKMSRLE
                     DVRLLDSFDYHKISSLSFEAREKLSRLRPRTIGQASRISGVTPSDISVLLIHTGR"
     gene            complement(3094..4599)
                     /locus_tag="PLE23_09700"
     CDS             complement(3094..4599)
                     /locus_tag="PLE23_09700"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HMX83578.1"
                     /translation="MSILKKLAGQTAIYGLSSIVGRLINFFLTPFYVRIFTEAENGIM
                     YDLFALTAFIMIVFTYRLETAYFRYATEPAYREKSFTTALYSILSTSIVFFSAIIIFA
                     DDIAVMLRYPDHPEYIRLFGGILVLDALVEIPLARLRLEGKAMKYATIKLLNIFINFG
                     LNFFVLYFLADAADGSGFAFLKPLADIFYNPEIRVGYVFIFNLLASASTFLLLLPYYR
                     ISLKEFDKKLLWKMLVYSLPLVVVSFAGIINEMLDRQLLKYILPYSNEENRAQLGIYG
                     ANYKLAMLLSLFTQAFRYAAEPFFFANAKDKKAPKLYALIAKYFTIVNLFGFLAIVLF
                     LHYFKYYVGSEGSRFWEGLRVVPILLMANIFLGLYYNISTWFKVTDNTGYGMLISLAG
                     AAITIGLNVWWIPIFGYMGSAWATLICYFSMTVITYILGMKYYPVKYDWLGIAAYMCF
                     AVALYMIHNFVVEYFQLGHLQTSLLSIAFIGLFSGIIIKLERKGYRESDVF"
     gene            4703..>5102
                     /locus_tag="PLE23_09705"
     CDS             4703..>5102
                     /locus_tag="PLE23_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HMX83579.1"
                     /translation="MVLNTTLLKKICETPGAPGFEHRIRQLIIKEIEPLVDSWYIDNV
                     GNLIALRKGSSDKKLMVTAHMDEISFIVTHIDDDGFIRFHPLGGFDPKTLTAQRVIVH
                     GTKDLIGVMGSKPIHIMKPEERTKAPAITDY"
BASE COUNT         1451 a         1196 c         1019 g         1436 t
ORIGIN      
        1 ataggggcat gccctaattg agccgtccgt agtgaaaaat catggtacgt accaattgta
       61 gttttcctgt tcggcatacc ggacatactg tcacgtcttt tccggtttgt agcatcagcc
      121 tcatggtggc ggatatggct acacgaggta agggtgtagg cagttgtaat tatttttctt
      181 gctgttgcta ccacgaattt tggcaccatc tacggccatt aatgtagcca attttaaaaa
      241 caacgcatct tcaaaggagg ctcttacagt ttttattttt ctgtttgata gagggattta
      301 atctctagtt ttttcaaatc caatttatct acaaaggcat ccataaatcg gattcattat
      361 cggtagaaat tgaatcttct agtgcataaa aagtgatttg ggaacggtca agacctgata
      421 agccaggcat tattgtaaga ttcactctat gtcttgaaat tacggaaagt ttttgtattt
      481 ttggtaaaat ttttaaaata tttttttcac agactgacga tagttacaat tacaaaaata
      541 taactaacgg aaatctactt ttattgaatc atgaaaaatg taatttactc tttatcagca
      601 atactcgtac tttttatatt tgattcctgc aaaaaggaag attctcctaa gccgcaaaat
      661 ctgatatccg gtaaatggca gtggcagcaa agtgtgggcg gattctctgg cagtgcagtg
      721 ctgacaccgg ctacagaagg atacaccagg cagtttaatt tcgggaaaga cagcgtaact
      781 attacactca atgatacttc ctttgaagac cgggcagcgt tccacctcac taacgaaaaa
      841 agcatactgt accataccac gcacgacttc ctgaccatag attatcattt taccattgga
      901 ggtgattcgg tcgttgtact gccgatgcgt tatatcatca aggatttgaa gcctgaaaaa
      961 atggttttga cagaagatgt ctttgacggg tttgagatta ctttggctaa gtagggcgac
     1021 atatatcact catagtatga tatgattttc cataacttcc ctattgcact tagcgagtaa
     1081 tcaactacct gcctgtgtgt atcagcagca cagagatatc ggatggtgtt acaccgctga
     1141 tcctgctggc ctgcccgatg gttcgcggac gcaacctgct gagtttttcc ctcgcctcaa
     1201 acgaaagact actaatcttg tgataatcga agctgtcaag cagccttaca tcttccagcc
     1261 tcgacatctt ttctgccatc tcgcgctctt tctggatata accttcgtac ttcatctcta
     1321 tctcggcaag gtttagtgtt tcatcatcaa acgccgaaag gtaatgctta agtgccggta
     1381 cggcatccat cattccatgc atactcacca ttggtcttga cagaacggag gcgagcttca
     1441 cttgttggtt aaggggcgca gaacccagct ctctcagata tccgttcatc agatcggggc
     1501 ttacgccggt ttcagtgaaa tgctttctga ttaacgaaat attcttcaac ttttcttcta
     1561 cgcgagccat gcgttcgtca agattgtgca taccaagttt gtgcagcaac ggggtcaaac
     1621 ggatatcggc attatcctgc ctcaataaga tacggtattc tgcccttgaa gtgaacatcc
     1681 tatacggctc ttcggtacct ttggtcacca ggtcatcaat cagtacaccg gcataggctt
     1741 cagagcgtgc cagtaccaat ggttcgaggt cgtggatagc tagatgcgcg ttgacaccgg
     1801 ctatgagtcc ctggcaggca gcctcttcgt agccggtagt accattgatt tgccccgcaa
     1861 aaaacagatt cttgaccagc ctggtttcca aagacatttg aagttgcgtt ggtgggaaat
     1921 aatcgtactc aatggcgtat cccgggcgga acatctttac attcttaaaa cctggtatat
     1981 aggtcatcgc tttgtactgc acatcttccg gcagtgaaga tgagaatccg ttaacgtaaa
     2041 tctcacaggt atctctgcct tccggttcaa taaaaagctg gtgtctttcc ttgtcagcaa
     2101 atcggtcaat tttgtcttct atagaagggc agtacctcgg tccgcgaccc tgaatacgac
     2161 ctgcaaacat cggtgaaaat tcaaatcccg tccttagtat ttcgtgcact ttttcattcg
     2221 tataagtgat gtgacacggt atctggttct cgagtcgtgg tgtatctgta aaagaaaagc
     2281 cggttgggtt ttcatctccg tgctgaactt ccatagctga atagtcgagg cttctgccgt
     2341 ctatcctggc tggtgtgccg gtcttcatcc tgcctgcttc aaagcctaac tccgtaagtt
     2401 gctcggtgat tcccgttgtc gatttttcac ctgcacgacc accaccaaat ctcttctcgc
     2461 cgatgtgtat gaccccgttg aggaatgtac cgttggtgag tactacagcc ttagacatta
     2521 tctctatacc catcgaagtg cgtacaccct ttacgacacc gttttttaca ataatcgagc
     2581 ttaccatttc ttgccagaaa tcaatgctgt ggttgtcttc aagcatcttg cgccacttga
     2641 gtgcgaatgc catcctgtcg ctttgagccc gcggactcca catagcagga cccttggaga
     2701 ggtttagcat cctgaactgt atcatcgtat gatcagtgac gatgccgctg tagccaccca
     2761 tggcatctac ttcgcgtacg atctgtcctt tggctacacc acccatagca ggattgcatg
     2821 acatttgcgc tatggtctgc atattcatag ttatgagtaa tacttttgaa cccatattgg
     2881 cagctgccac agctgcttca catccggcat gacctgcgcc tacaacaatc acatcgtatt
     2941 caggaaacat tcagtgcaat ttttctgcaa atttaaggcg aatagatgaa gcaagcagat
     3001 ttttttcttc taccatcata ttttaaccaa aatacaacaa ctttcatact gcatggttca
     3061 gtattaattc attgcaaagt tgctttccac accctaaaac acatccgact ctcgataccc
     3121 ctttctctct aacttgatga taataccgga aaataaaccg atgaatgcta tactcagcag
     3181 gctcgtctgt aagtggccca gctgaaagta ttcaactaca aaattgtgaa tcatatataa
     3241 ggcaaccgca aaacacatat aagccgctat acccagccaa tcgtatttta caggataata
     3301 cttcatgcca agtatgtaag ttattacggt cattgaaaaa tagcaaatca atgtagccca
     3361 ggcactgccc atatatccga atatcggtat ccaccagacg tttagtccga tggtaatggc
     3421 agcacctgcc aacgatatca acattccgta acctgtgttg tccgtcacct taaaccatgt
     3481 agaaatgttg tagtacaaac ccagaaaaat attggccatc agcaatatcg gtaccacacg
     3541 cagcccttcc cagaaacggc ttccttcgct gcctacataa tatttgaaat aatgcaggaa
     3601 cagtacgatt gccaaaaaac cgaaaaggtt gacaatggtg aaatatttag caatcaaagc
     3661 atacaatttc ggtgctttct tgtccttcgc attcgcaaaa aagaaaggct cggctgcata
     3721 cctgaatgcc tgcgtgaaca atgatagcaa catggcaagc ttataattgg caccatatat
     3781 acccagttgt gcacggtttt cttcgtttga atacggcaaa atgtacttca atagctgtct
     3841 gtcaagcatc tcgttgatga tgcccgcaaa acttactact accaagggca gcgaatagac
     3901 aagcatcttc catagcagtt tcttgtcaaa ttccttcagc gatatcctgt aatatggcaa
     3961 tagcagtaaa aatgtacttg cagatgcgag aagattgaaa atgaatacat acccaacccg
     4021 tatttccgga ttataaaaaa tatcggccaa aggcttcaaa aacgcaaatc cgctaccatc
     4081 cgctgcatct gccaggaagt acaacacaaa gaagttgagc ccgaaattga taaagatatt
     4141 caacaacttg atcgttgcat acttcatggc ctttccttca agcctgagtc tcgccagcgg
     4201 tatctcaacc agcgcatcca gtaccaatat accaccgaat aacctgatgt actccggatg
     4261 gtcaggataa cggagcatca ccgctatgtc atcagcaaat ataattattg ccgaaaaaaa
     4321 tactatactc gtactcagta tagagtacaa tgcagtggta aaggattttt cacggtatgc
     4381 cggttctgta gcatacctga agtaagctgt ctcaagtctg taagtaaaca caatcatgat
     4441 aaatgccgtg agcgcaaaca gatcgtacat gatgccgttt tcagcttctg taaaaatccg
     4501 tacatagaat ggcgtcaaaa aaaaattgat cagcctgcct acaatgcttg acaacccata
     4561 gatggcagtc tgacctgcaa gtttctttaa tatcgacata aattcatgta aaccacacgg
     4621 taaaagtaag gctttgcttc ggataaaagc catattttcc ccacgtttgt gtaaaacttt
     4681 gtaattttcg cacaaaaact gaatggtact caatacaact ctactcaaaa aaatatgcga
     4741 aacgcccgga gcacccggtt tcgaacatag aattcggcag ctgatcatta aagaaataga
     4801 accacttgtc gatagctggt atatagataa tgtcggcaac ttaattgctt tacgcaaagg
     4861 aagttctgac aaaaaactga tggtcactgc acacatggat gaaatcagct tcatcgtcac
     4921 gcatattgat gacgacggct tcatccgttt tcatccgctg ggtggcttcg accctaagac
     4981 gctcacagca cagcgtgtca tcgttcacgg taccaaagac ctcatcgggg tcatgggttc
     5041 taagcccatc cacatcatga agcccgaaga gcgcactaag gctcctgcca ttacagatta
     5101 ct
//
LOCUS       DAOQQY010000211         5101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7460_length_5101_cov_4.860285, whole genome shotgun sequence.
ACCESSION   DAOQQY010000211 DAOQQY010000000
VERSION     DAOQQY010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7460_length_5101_cov_4.860285"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /gene="groL"
                     /locus_tag="PLE23_09710"
     CDS             complement(<1..1124)
                     /gene="groL"
                     /locus_tag="PLE23_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMX83580.1"
                     /translation="MANKEISFNTEAREKLKSGVDALANAVKVTLGPKGRNVVIQRSF
                     GAPTVTKDGVSVAKEIELEDAVQNMGAQMVKEVASKTADIAGDGTTTATVLAQAIVNT
                     GMKNVTAGANPIEIKRGIDKATKAVISQLKEQAEVIGSDFEKIKQVASISANNDEEIG
                     TLIADAMKRVSTEGVITVEEAKGTDTYVEEVMGMQFDRGYLSPYFVTNAEEMVAEYEN
                     PYILIHDKKISNMQDLVPILEKVIGAGRSLLIIAEDIESQALGVLVVNRLRAGLKVVA
                     VKAPGFGDRRKAMLEDIAILTGGTVISEEQGYKLENTELSHLGSCNRITVDKDNTTIV
                     DGKGSQDDIKSRINQIKQQIEKTTSDYDREKLQERLAKLAG"
     gene            complement(1146..1409)
                     /locus_tag="PLE23_09715"
     CDS             complement(1146..1409)
                     /locus_tag="PLE23_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMX83581.1"
                     /translation="MKPINDRVVVRPAAAEEKTKGGIIIPDTAKEKPQRGEVVAVGPG
                     KDGNLMTVQVGDIVLYGKYAGQELNFEGHEYLIMREDDILVIL"
     gene            complement(1705..3333)
                     /locus_tag="PLE23_09720"
     CDS             complement(1705..3333)
                     /locus_tag="PLE23_09720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HMX83582.1"
                     /translation="MMKKILFGLFSLIFMQSAFSQDKELPKNWFNLDPATDGYPGMST
                     EKVYNTLLQGKTGVPVIVAVLDGGVDYMHEDLKDIMWKNPKEIPGNNIDDDRNGYIDD
                     VYGWNFLGGRDGKNVGVDNLEITRIVRAKRNKFSNVDPKNLSGKDKKEYEEYLKLKDI
                     VDTQKKNAESSLKLYSEMQTNLRNAESKLKAYFGKDVITLEMLDLIKNTDSKELKDAV
                     ALMSNALKNGFTADDLQDAVEHFSKQADYQYNVDFNPRNIIGDKYANSSERYYGNADI
                     KGPESFHGTHVAGIIAANRTNNLGIQGVANNVQIMGVRCVPDGDEHDKDVANSIRYAV
                     DNGAQVINMSFGKSYKWDKKAVDDAVKYAESKDVLLVHAAGNDSKDNDKNDNFPTDKF
                     EKAGLFAKKYASNWLEIGAASYKTGENAVAGFSNYGKKNVDVFAPGVQIYSTIPESKY
                     GFAQGTSMAAPMVAGVAAVIRSYYPELTAAQVKKIIMDSSIKSNQQVKKPGTRGTMVS
                     FSELSVSGGDVNLYEAIKLASKTKGKKKAQSPRS"
     gene            complement(3444..>5101)
                     /locus_tag="PLE23_09725"
     CDS             complement(3444..>5101)
                     /locus_tag="PLE23_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83583.1"
                     /translation="NYSFTYTTNSATVPCSESSYTIEVIVEDCECPSVATKNPGNFCS
                     DDADYNLDNIKLTNEAGTWSIISQPPGSNPANIVGNMFEGTDADPGNYTILFTLNTTP
                     PPGCASSSQQSFKISESLTAGNTPQQISICANDAQLINLNASLQGASPNGIWTEISAN
                     PSKNNVQNIQNGFFDTKNQATGLYQLEYKVVPAMPCTPQSVVLSIIINENPVADAGPD
                     KLITCAEPSVSIGSNNSTTGAGISYNWSGNVTNAKSLTTQTAVPGIYQLTVTNTSTTC
                     FSTDNVTVTLANDLPLPSNLEIDSINCFGQSDAKIKFDNIQGGTSPYTVFLNNVDFGS
                     AQYFTKLPPGTYELKISDANGCTSTQTITITEPEKIYLELGSDVTVELGDSVHIEPFI
                     NIPLSNIDTIIWTPLINGPNGVCAGCFDHVLYPYYTTPLEMLVIDNKGCRVSDQMHIT
                     VDVVRQIFFPNIFSPNEDALNERFYPFADDRVVKIHEFRVFDRWGNMVFEQTNFLPND
                     YDKGWDGTRNGKHLNAGVYVYYAVVEFRDGAKKVFKGDVTLVH"
BASE COUNT         1332 a         1065 c          927 g         1777 t
ORIGIN      
        1 cctgcgagct tggcaagcct ttcctggagc ttttcgcgat catagtcaga tgtagttttt
       61 tcaatctgtt gtttgatttg gttgattctt gatttgatat catcctggct acctttaccg
      121 tcaacaatcg tagtattgtc tttatcgaca gtgattctgt tgcaagagcc aaggtgtgac
      181 aactcggtat tctcgagttt ataaccttgt tcctcagaga ttacagtacc accggtaagt
      241 atggcgatgt cttcaagcat tgcttttctt ctgtcgccaa aacccggagc ctttacagct
      301 acaaccttta agccagcccg aagcctgttc actacaagta ctcccaaagc ctggctttct
      361 atatcttcag caatgataag cagtgacctt cctgcgccga ttactttctc gagaatcgga
      421 acgaggtcct gcatgttcga gattttctta tcgtgaatga gaatataagg gttctcgtat
      481 tcagctacca tttcttcagc attggtaacg aaatacggag acaaataacc tctgtcgaac
      541 tgcataccca taacttcctc aacataagta tccgtacctt tagcttcttc aactgtgatg
      601 acaccttctg tagaaaccct tttcatggca tcagcaatga gtgtaccgat ttcttcatcg
      661 ttattagcag aaatggaggc tacctgcttg attttttcga aatcacttcc tattacttca
      721 gcctgttctt ttagttgact gatgacagct ttggttgctt tatctatacc acgtttgatt
      781 tcgatagggt ttgcgccggc tgttacgttc ttcataccgg tgtttacgat agcctgggca
      841 agtacagtag cagtagtcgt accatctccg gctatatctg ctgtttttga agcaacttct
      901 ttgaccattt gagcgcccat gttctgtaca gcatcttcca gttctatttc cttagccact
      961 gagacaccat ctttggtaac agttggggca ccaaaagatc tctggataac gacatttctt
     1021 cctttcgggc caagtgtaac ttttactgca ttagcaagtg cgtcaacacc actttttagt
     1081 ttttctcttg cttctgtatt gaagctgatc tctttgtttg ccatatttta tttgtgttta
     1141 aatatttaaa gaattaccag aatatcatct tctctcatga taagatattc atggccttcg
     1201 aaattaagct cctggccggc atatttgcca tatagaacaa tatcacccac ctgaacagtc
     1261 atcagattac cgtctttgcc ggggcctact gctaccactt ctccacgctg tggtttttct
     1321 tttgctgtgt ccggaatgat aatgcctcct ttagtttttt cttctgctgc ggcaggtcgt
     1381 accacaactc tgtcattaat tggtttcata aataatattt tagtttacag attaattatt
     1441 gtttacaaat ggtaaatact acccatgtct tatcattaaa tgtgccacac atacaaaact
     1501 gtcaaaattt ctgaacttgc ttgtatctat ggcagtattg cttgattttt ctttcaatgt
     1561 ttctgaaaaa atggcagaat tgatttttac ttgaagcatt ttttggtaaa aataaaagaa
     1621 gtagcatttt gctatcaaaa aaacaaaagc ccgatatatg ctatcgggct tttgtttttt
     1681 tgatagctta aagcatcagg ataattaaga ccttgggctc tgagctttct ttttaccttt
     1741 ggtttttgat gccagtttga tggcttcata gagattgaca tcaccacccg atacacttag
     1801 ttcgctgaat gataccattg tacctcttgt gcccggcttt tttacttgct ggttgctttt
     1861 gatactcgaa tccataatga ttttcttaac ctgtgctgct gtcaattcag gataatatga
     1921 tcgaattact gccgccacac ctgcaaccat tggggctgcc atacttgtac cttgggcaaa
     1981 accatatttg ctttcaggaa tggtagaata tatctgtaca ccaggtgcaa agacatcaac
     2041 attctttttg ccataattag aaaatccggc tactgcattt tcacctgtct tataagaggc
     2101 agcacctatt tctaaccagt tggaggcata tttcttagca aataatccgg ccttttcgaa
     2161 tttatctgtt gggaaattat catttttatc attgtcttta ctgtcattac ctgccgcatg
     2221 taccaacagt acatctttcg attctgcata tttaactgca tcatctactg cttttttatc
     2281 ccatttgtac gatttgccaa agctcatatt gattacctgt gcgccattgt caactgcata
     2341 tctgatagag ttggctacat ctttatcgtg ctcgtctcca tcaggtacac aacgtacacc
     2401 cattatctgc acattattgg ctacaccttg gatgcccaga ttgttggttc tgttagctgc
     2461 aataatacct gctacgtgtg taccgtgaaa tgactctggg cctttgatgt cggcattacc
     2521 ataatatctc tccgaagaat tggcgtactt gtcacctata atatttcttg gattaaaatc
     2581 aacattgtac tgataatcgg cctgtttgct gaaatgttcc accgcatctt gcaggtcatc
     2641 tgctgtaaat ccatttttga gtgcatttga cattaaggct actgcatctt taagttcttt
     2701 agaatcggtg tttttaatca aatcaagcat ttcaagtgta ataacatctt taccaaaata
     2761 agctttaagt ttgctttcag cattccgtaa attggtctgc atttcagaat ataatttcaa
     2821 gctgctttca gcattttttt tctgagtgtc aacgatgtct tttaacttga gatattcttc
     2881 atattctttc ttatccttac cactcaagtt tttaggatca acatttgaaa atttatttct
     2941 ttttgcacgt actattcttg ttatttccag attgtcaacg cctacatttt taccatctct
     3001 gccaccaagg aaattccatc catacacatc atcaatataa ccgtttctgt catcatcaat
     3061 attatttcca ggtatttcct ttggattttt ccacatgatg tctttcaagt cttcgtgcat
     3121 gtaatctact cctccgtcaa gaacagcaac tatcacaggt acacctgttt tgccttgcaa
     3181 taatgtgttg taaactttct ctgtactcat acccggataa ccatctgtag ccggatcaag
     3241 attaaaccaa ttcttaggta attccttgtc ctgactaaat gccgattgca taaaaataag
     3301 tgagaacaag ccgaataaaa tttttttcat catggatatt tttaataaat ttgaattaaa
     3361 acgtaaaatt aagcaatatc gtacttaatc gctccaagat ggtcgatgaa atttaagctt
     3421 ttgtctaagc gcagtacttt atcttaatgc acgagggtta catcaccttt gaataccttc
     3481 ttcgcaccgt ctctaaactc tacaactgca taataaacat acacgcctgc attcagatgt
     3541 ttgccattac gtgtaccgtc ccaacctttg tcatagtcgt ttggtaagaa gtttgtctgt
     3601 tcaaatacca tattacccca tcggtcaaat actcggaatt catgtatttt gacaactctg
     3661 tcgtctgcaa aagggtaaaa acgctcgttg agtgcatctt catttggtga aaagatgtta
     3721 gggaaaaata tctgccgtac aacatctacg gtaatatgca tctgatcgct tactctacat
     3781 cccttgttgt caattactag catttctaat ggggttgtat aatatgggta taatacatga
     3841 tcgaaacaac cggcacacac accgtttgga ccgtttatca aaggtgtcca gataatagtg
     3901 tctatattgc ttaaaggaat attgatgaat ggttcgatgt gtacactgtc acccaactca
     3961 actgtgacat ctgagccgag ttcgagatag attttctcgg gttctgtaat tgtgattgtt
     4021 tgtgttgatg tacaaccatt ggcatctgat atctttaatt cgtaggtgcc aggtggcagc
     4081 tttgtaaaat actgtgcact gccaaaatct acattattaa gaaacactgt atatgggctt
     4141 gtaccacctt gtatattatc aaattttatc ttggcatctg actgaccaaa acaattaatg
     4201 gagtcaattt caaggttgga tggcaaaggg agatcattgg ctaaagtaac tgtaacgtta
     4261 tctgttgaaa agcaagtagt gcttgtgttg gtgactgtta actggtaaat gccgggtaca
     4321 gcagtttgtg tggttaatga ttttgcattt gttacatttc ctgaccaatt gtagcttatt
     4381 cctgcaccag ttgtactgtt gttgctacct atgcttactg atggttctgc acatgttatt
     4441 agtttgtcag gcccggcatc tgctaccgga ttttcattga ttattatcga caatactact
     4501 gattgtggtg tacatggcat tgctggaacc accttgtatt caagttggta aagtccggtt
     4561 gcctgatttt tggtatcaaa aaaaccgttc tgtatgttct gtacattatt tttggatggg
     4621 ttagctgata tttccgtcca aataccattc ggtgatgctc cttgcaaact cgcattgaga
     4681 tttataagtt gtgcgtcatt ggcgcatata gaaatttgct gaggagtatt tcctgccgtc
     4741 aaactttctg aaattttaaa gctctgctgg ctagaactgg cacatcctgg aggcggtgtt
     4801 gtattcaggg taaaaagaat tgtataattg cccggatcag catcggttcc ttcaaacata
     4861 tttccgacaa tattggcagg attgctgccg ggtggctgtg aaatgatact ccaggtacca
     4921 gcttcatttg ttaattttat attgtcgaga ttataatctg catcatctga gcaaaaattg
     4981 cccgggtttt ttgtagccac acttggacat tcacagtctt ctactataac ttcaatcgta
     5041 taggaacttt cactgcatgg gacagtagca ctgttggtag tataagtaaa gctataatta
     5101 c
//
LOCUS       DAOQQY010000212         5069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7533_length_5069_cov_7.471679, whole genome shotgun sequence.
ACCESSION   DAOQQY010000212 DAOQQY010000000
VERSION     DAOQQY010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7533_length_5069_cov_7.471679"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4212
                     /locus_tag="PLE23_09730"
     CDS             <1..4212
                     /locus_tag="PLE23_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83584.1"
                     /translation="ILSIAISAQPCPCTVNAGADKNLCEPGGTTQLNGSYTGSPLVIE
                     WTPAIGLNDPNIINPEATVDQTTTYTLKIKCLNNTNLVTNANFNNGNTGFSSDYVYSA
                     TNLVPEGVYTVTNNPKSVHPGFAPCGDHTGGGQMMVINGAGTPGLNVWCQTIPVKANT
                     DYAFETWVASVVAGSPALLQFSINGQAIGPIFSANNSNCIWEQFTAVWNSGSSTSATI
                     CIVNQNTELGGNDFALDDISFKELCEITDEMTVFVHPTKQTDIEAKICQGQSYNIGGQ
                     SLQNEGQYNFSFKTWKGCDSLVTLDLSVIEVEAVIDNPEMLDCNSSEILLYGDKSSFG
                     AFYTYAWTGPPGGISSDPSQSIVTVTKPGVYTLVVTYDDGTIKCTKSATVTVTTDYTK
                     PIITAGPDGKLSCTDTTLTLSGKVTSPTSNYTILWTTPDGNIISDPDVLDPLIGSTGT
                     YIMTVTNNLNGCTASDIVLVTKDPSVPKVKISGDTLLTCNNDTIWINGFQSDQGAGFD
                     YEWSVANGGKINTTTDSLAIQLSTPGSYILTVQNLTTGCKSNFTLNIGEDKSSPTAEA
                     GQKDTLSCLIPELQLTGSSNLDISLININWTTPDGNIKSGSDLFNPIISSPGIYYLNI
                     ENKKNGCIAQDSVNIAKDDNAPSVIKLQDQTLTCVVKNVPLDATGSSTGSDFVFSWTT
                     PDGNITGGTDVLVSQCDKAGMYILNITNTKNGCVVADTLWVILDDKKPVADAGSDKTL
                     NCNNTSLQLDGTLSSSGIQYSYQWTTPNGNIINGAATVKPLIDKPGTYTFVVTDAGNG
                     CTSAADVNVSIDTVAPVITTPGDLILTCQQPQILISAINLSGSGNYSYLWSTQNGNII
                     SGENTLTITIDKPGTYTFVTINDNNGCMSSGTVKVTSNSELPEVNAGQDLIINCINTQ
                     VSAAASVIYSGGTLDIIWTSSTQPVISDGNTLNPVLGYAGTYTITVLDPVTGCSASDK
                     LVVTIDTIAPKSSIPTPELLTCTRLTSLVSLQTILPTWTYSWSTADGNITGSTTGNSV
                     TVDVTGLYSVTITDQSNGCISVLSGTVNEDKKNPVVSAGPDYELTCTIEEVTLAGNIQ
                     GGTTNKTILWLYNNQTLQNGTTLNPVVNLPGSYLLVVTDNINGCTADDSAEVTENTNY
                     PKDFAIDIIPPGCTKLGTLSVSAVIGGNEPYTYSLNGGVFQNSDVFESIKSGTYFINI
                     KDANGCEFGKQIMVPEPPELSVTLPQLVTIEYGKDEILNPQLNIPESYVESALWTPVS
                     GLSCGDCLTPFANPVNETTYSLIVTDVTGCTAQAKVKVIVLKDFKLYIPNAFSPDADG
                     INDKWMLFGDPKIVVNIKELRIFDRWGEQMYEAFNFPVNDPQYGWDGKLRGKNLKPAV
                     FVYYFEAEFVDGSRRLYKGGINLIR"
     gene            complement(4217..4543)
                     /locus_tag="PLE23_09735"
     CDS             complement(4217..4543)
                     /locus_tag="PLE23_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HMX83585.1"
                     /translation="MWTIILLVFSNMFMTFAWYGHLKDPSIPMWKAVLISWGIAFLEY
                     CFMVPANRIGYGTFNAFQLKTIQEVITLVIFIAFSVIYLKEQLRWNHIVAFVFILAAV
                     YFMFKK"
BASE COUNT         1581 a         1056 c         1043 g         1389 t
ORIGIN      
        1 atactctcaa ttgccataag tgcgcagccc tgcccatgca cagtaaatgc cggagcagat
       61 aaaaatctat gtgaacccgg cggtacaaca caactcaatg gtagctatac aggttcacct
      121 ttagttatag aatggacgcc tgctattggc ttgaatgatc caaatatcat caatccggaa
      181 gcaacagttg accaaactac cacttacacc ttgaaaatta aatgcctgaa caataccaat
      241 cttgtaacca acgctaactt caacaacggc aataccggtt tttcgagtga ttatgtgtac
      301 agcgccacaa atctggtacc cgaaggtgtt tatacagtta ccaataaccc aaaaagtgtt
      361 cacccgggtt ttgcaccttg tggtgatcat acaggtggtg gtcaaatgat ggtgataaac
      421 ggtgcaggta cacccggcct taacgtctgg tgtcagacaa taccagtaaa ggcaaatacc
      481 gattatgcct tcgaaacctg ggtggcatct gtagtagcag gctcaccggc tttgttacag
      541 ttttctatta atggacaggc cattgggcca atattcagcg ctaataattc gaattgtatc
      601 tgggaacagt ttacagcagt ctggaatagt ggaagttcta catctgctac catttgtatc
      661 gtaaaccaaa acacagaatt aggcggaaat gactttgcac ttgatgacat ttccttcaag
      721 gagttgtgcg agataacaga tgaaatgaca gttttcgtac atccaaccaa acagacagat
      781 attgaagcaa aaatttgtca gggacagtct tataacattg gcggccaaag cctgcaaaat
      841 gaaggacagt ataacttttc tttcaaaaca tggaaaggat gcgatagtct ggtaacactt
      901 gacctgagcg ttatagaagt agaagccgtt atcgataatc cggaaatgct tgactgcaat
      961 tcgtccgaaa tcctgctata tggagacaaa tcttcatttg gggcattcta tacttatgcc
     1021 tggacaggac cacctggtgg aataagttct gacccttcac aatccattgt aacggttacc
     1081 aagcctggag tctatacgct tgtagtcacc tacgatgatg gtacaattaa atgcaccaaa
     1141 tctgcaacgg taactgtaac aactgattat actaaaccta tcattacggc agggcccgat
     1201 ggtaagcttt catgtaccga caccacgctt acattaagcg gaaaggtaac gagccctaca
     1261 agcaactata ctatactgtg gacgactcct gacgggaata ttatatctga tccagatgtg
     1321 ttggatccgc tcattggcag cactggcacc tatatcatga cagtgactaa taatctgaat
     1381 ggttgcacgg caagtgacat tgttctggtg acaaaagatc cttcagtacc aaaggtaaag
     1441 ataagtggag atactttgct tacatgtaac aatgatacca tttggatcaa tggttttcag
     1501 tccgaccagg gtgcaggatt cgattacgaa tggtcagtag ccaatggtgg aaaaattaat
     1561 acaactactg actctctggc tatacagctt agcacaccgg ggagttatat tttaactgta
     1621 cagaatctga ccaccggctg caaatcaaat tttacactaa acatcggaga ggacaaatca
     1681 tcgccaacgg ctgaggcggg gcagaaagac acactttctt gtcttattcc tgagttgcag
     1741 cttaccggtt catctaactt ggatatttca ctcatcaata ttaattggac cacaccagat
     1801 ggtaatatca aatcaggtag cgatctcttt aatccgatta tttcttctcc cggtatttat
     1861 tatttgaata tagagaataa aaagaacgga tgtatagcgc aggattcggt taacatcgcc
     1921 aaagatgata atgcaccttc agtcattaaa ctgcaagacc agacacttac ttgtgtggtt
     1981 aagaatgtcc cactcgatgc tacaggttca tcaacaggca gtgattttgt attttcgtgg
     2041 actacacctg acggaaacat caccggaggt acagatgtgc tggttagcca gtgtgataaa
     2101 gcaggtatgt atatattgaa cataaccaat acaaagaacg gatgtgtagt ggcagacacc
     2161 ctatgggtaa tattggatga taaaaaacct gtagcggatg ccggttctga taaaacactc
     2221 aattgcaata acacaagtct gcaacttgat ggtacactca gttcatcggg catccagtac
     2281 agttatcagt ggactacacc aaatggtaat attatcaatg gtgcagcgac tgttaagcca
     2341 ctaatagata agccgggtac ttacacattt gtagtgacag atgcaggcaa tggttgtaca
     2401 agtgcagcag atgtaaacgt aagtattgat accgttgcac ctgttattac cacacccgga
     2461 gatctgatac ttacctgcca acaaccgcag atactgatat cggccataaa tctttctggg
     2521 tcaggcaatt attcgtactt atggagtacc cagaacggaa atatcatatc aggagagaat
     2581 acgttgacaa ttaccattga caaaccaggc acatacacat ttgttaccat taatgataac
     2641 aatggttgta tgtcttccgg cacagtaaaa gttacaagca attccgaact gcctgaggta
     2701 aatgcaggtc aggatcttat aatcaactgt atcaatacgc aggtatctgc cgctgcaagc
     2761 gtcatttact cgggcggtac actcgatatc atttggacct ccagtactca gccggttatc
     2821 tcagatggca atacactcaa tcctgtttta gggtatgccg gcacctatac cattacagtc
     2881 ctggatcctg tcacaggttg cagtgcaagc gataaactgg tggtcactat tgataccatt
     2941 gctccaaaat catccatccc aacgcctgaa ttactcactt gcacaagatt aacctccttg
     3001 gtcagcctac agacaatcct tcctacgtgg acgtacagtt ggagtacagc agatggcaat
     3061 atcacaggta gtacaaccgg caattctgtt acagtggacg ttaccggatt gtattcggtt
     3121 accattaccg accagtccaa cggctgtatt tctgtgttat ccggtactgt caatgaagat
     3181 aagaaaaatc cggttgtgtc agcaggaccc gattacgagc tgacatgtac catagaggaa
     3241 gtgacacttg caggaaacat acaaggaggc acaactaata aaaccattct gtggctttat
     3301 aataaccaga cattacaaaa tggcacaaca ctcaacccgg ttgtcaattt gccgggtagc
     3361 tacctgctcg tagtaacaga caacatcaac ggctgtacag cggatgatag tgctgaagta
     3421 acagaaaata ccaactaccc gaaggatttt gccatagaca tcatcccgcc cggttgtacc
     3481 aaactgggga ctttatccgt ttcagcggtt ataggtggca atgaacctta cacctactcc
     3541 ttgaatggcg gagtattcca gaattcggac gtatttgaat ccattaaaag tggaacttat
     3601 tttatcaaca taaaagatgc caatggctgt gagtttggca agcagattat ggtgcccgag
     3661 cctcccgaac tatccgttac cttgccacag ctcgtgacca ttgagtatgg caaagatgaa
     3721 atactgaatc cacagctcaa tattcctgag tcgtatgtcg aaagtgcatt gtggacaccg
     3781 gtatccggac tttcatgcgg cgattgtctg acaccttttg ccaatcctgt caatgaaacc
     3841 acctatagcc ttatcgtcac agatgtaacc ggctgtactg cccaagcaaa ggtaaaggtc
     3901 attgtactaa aagatttcaa gctatacata ccgaatgcct tttccccaga tgctgatggc
     3961 attaatgata aatggatgtt attcggtgat ccgaagattg ttgtaaatat aaaagaattg
     4021 cgaattttcg accgctgggg cgaacaaatg tatgaagcct tcaattttcc ggtaaacgac
     4081 cctcagtatg gctgggatgg taagctgcga ggaaaaaacc tgaagcctgc tgtatttgta
     4141 tattactttg aagctgaatt cgtagatgga agcagacgat tatacaaagg tggtattaac
     4201 ctcatacgct aacggattat tttttaaaca taaaataaac tgctgcaagt ataaagacaa
     4261 atgccacaat atggttccag cgtagttgct ctttgagata aataaccgaa aatgctataa
     4321 aaataacaag agtgataact tcctgaatgg tttttaattg aaatgcgttg aaagttccat
     4381 aaccgattcg gttggcagga accatgaaac agtattcaag aaatgctatt ccccaactga
     4441 tcaataccgc cttccacata ggtatcgatg ggtctttgag gtgtccgtac catgcaaagg
     4501 tcatgaacat attcgaaaac acaagcagga ttatggtcca catagagttg aaattttagc
     4561 gggtgattca aatttttggt aaaaatagat tctgcccgtc ttatctgttt ctgttgagga
     4621 gcaatatttt ttttattaaa aatgcagccg aaatttcagc aagattttct attgaaattt
     4681 gcataaatcc ataggatgac aaaaaagcat ttattccaag ttatgtgttg aaaatctgct
     4741 gtttatattt ttattataat tttattatgt aaaacagaaa aatgatgccg gccaacattt
     4801 ctgtctgaac ctgtgtttga catttaacac agcgtttctg cacgaaccac ttgcatctta
     4861 atctttttgg tatgacattt gtgctttaat tgacattgaa atcaaagcac tttttgctaa
     4921 attttttaaa aatgaatcaa aacattatca gttacaaaag gaatagcttc ggagcaatga
     4981 catgttgttg ttgctgttgt tatatacctg agcttagtat cggataaaag aggatataca
     5041 ttatttacac atcaaaaaaa tccaccggt
//
LOCUS       DAOQQY010000213         5060 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7551_length_5060_cov_7.240360, whole genome shotgun sequence.
ACCESSION   DAOQQY010000213 DAOQQY010000000
VERSION     DAOQQY010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7551_length_5060_cov_7.240360"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..1082
                     /locus_tag="PLE23_09740"
     CDS             15..1082
                     /locus_tag="PLE23_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMX83586.1"
                     /translation="MKKNLTNVLKALGFMAIGMTILFLLYQSQNKAFQAQCVTDGISK
                     ENCSLIKKLLDDFRQTNPFWIILIVITTLLSHLARALRWNQLIESLGEKPRIINGLFS
                     VIIGFFANLGIPRIGEIVRATTMAKYENIPTEKLFGTVVVDRIVDMITFIILVGVTFF
                     LEFDTISTKLGELMATNQSKNTAAPSNLWYLLVGLCIIGVALIIFYIKRPENLMVKKV
                     KGLVTGVLEGIATISKLERPWLFLFYTVAVWFLYYIMLYLCIPAFGPTRHLGMQAALI
                     VFVFGAFGFLVPSPGGMGTYHWLIIQALTLFGIQAADGFSFANIAYFTGQIISNVGFG
                     LLALILLPIVNKNYHPNTNKS"
     gene            1079..1573
                     /gene="rfaE2"
                     /locus_tag="PLE23_09745"
     CDS             1079..1573
                     /gene="rfaE2"
                     /locus_tag="PLE23_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HMX83587.1"
                     /translation="MSFQSEYNKKIKDLLSMTRQVNVWRTKPVKIVFTNGCFDILHTG
                     HVQYLEEAKSLGDKLIIGLNSDASVKRLKGAERPVNHQEDRANVLAALGFVDGIVIFD
                     EDTPAELIEALCPDILVKGGDWPIEKIVGSNFVLNNGGQVLSIPFREGHSTSGTIEKI
                     KKLG"
     gene            1573..2673
                     /locus_tag="PLE23_09750"
     CDS             1573..2673
                     /locus_tag="PLE23_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HMX83588.1"
                     /translation="MTTIRDIIGHLESIAPPYYQESYDNSGLVVGDPATAVSGVIICL
                     DSTEEVVQEAIEKKCNLIIAHHPIIFGGLRKINGKDYVEKTIIKAIKHDIAIYAIHTN
                     LDNVLENGVNQKISEKLGLQNCRILLPKTQTLVKLTSFVPNNARDKVLNAVCSAGAGQ
                     IGNYKNCSFYTTGTGSFTPTGNANPFLGEINKPEQVEESRIEVILPEYLSQAVLNALK
                     EAHPYEEVAYYLQKLENTDTNIGSGIIGTLEHPVASDFFLEQIKEKLGVTCIRYTKLV
                     KSSFCKVAVCGGSGSFLLKHAIAQGADIFISADFKYHDFFDANNKITIADVGHFESEQ
                     HTIQLLFDIINKKFSNFAVHLTQVITNPVKYL"
     gene            2676..3443
                     /locus_tag="PLE23_09755"
     CDS             2676..3443
                     /locus_tag="PLE23_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HMX83589.1"
                     /translation="MSKNDISVSDKLKRLYELQLVDSELAELETMKGELPMEVADLED
                     EIAGLQTRLQKIDHSVDEVKSDINKHNTSIKDSEALIAKYQKQLDNVKNNREFEALTK
                     ELELQKLEVQLHEKKIRELNGGIQAKLELQQGTADKLAQKKGDLDLKKSELATIIAKT
                     EKDEEKLRKKSEKASKTIEDRLLKAYSKIKKAYHNHLAVVTVTRNACGGCFNKIPPQL
                     QLEIALRKKIIACEHCGRILVDEHILEAEVAPVNTPE"
     gene            3527..5017
                     /locus_tag="PLE23_09760"
     CDS             3527..5017
                     /locus_tag="PLE23_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83590.1"
                     /translation="MIAMTMATANIQLQAIVSTDYDFDFNEDINNAYNLVTALRLDEA
                     RNALHQLKQTQPNNLAPIFIEDYIDFLCLMLSEDKALLRNYQINFNNRLALIDKNCFK
                     HSPYYLFIKSEMTMHRAAVDFRFGNNILAFTAALKAYKLLETNRTLFPDFVPNLKNLG
                     LMQIIVGSMPPSFKGGFSFFTGISGSVVIGSQDVKRVMNEKSRQSYIFAFEAKVLYSF
                     IQLYINDEPDEAWALLRQTKVNYHAQPLLAFIHAGASYRSKRNDECIRLLTENNYGTD
                     YYPITISNLILGRAKLNRMDKDANIFLERFLKQYKGTTFRKEAHLLLHWYYTLFPDQK
                     KREQHYQAVFSDGNTNADSDNAAMQELKANHVNSQVFLRARLLYDGGYSQRALTELIL
                     AKSGIKNNPSLLLEYNYRYARILQQMNNIQDAIRLYRQVITTGRSRSEYFACNAALQI
                     AVIYEKTNQPANSEFYYNECLRINPATYKNSLHGKAKAGLLRLKQK"
BASE COUNT         1683 a         1039 c          970 g         1368 t
ORIGIN      
        1 taatctaaat tctcttgaaa aaaaacttaa ccaatgttct gaaggctctg gggtttatgg
       61 ctattggaat gacaattctg tttctcctgt accaaagcca aaataaagct tttcaagctc
      121 aatgtgttac agacggaatt tcaaaagaaa actgcagcct gattaaaaaa ctcctggacg
      181 acttccgcca gaccaaccca ttttggatta tactcattgt tatcaccaca ttactcagtc
      241 atctggcaag agctttgcgc tggaaccagc tcatcgagtc acttggcgaa aaacccagaa
      301 taatcaatgg cttgttttct gtaatcatcg gcttttttgc caatctgggt attcctcgta
      361 tcggcgaaat agtacgtgct accactatgg caaaatatga aaatatacct accgaaaaac
      421 tcttcggcac cgtggtggtg gacagaatcg ttgatatgat tactttcatc atacttgtag
      481 gcgtcacatt tttcctcgaa tttgatacca taagcaccaa attgggcgaa ctgatggcca
      541 cgaatcaaag taaaaatact gccgccccta gcaatctatg gtaccttctt gtaggattat
      601 gtataatagg tgtagcactc atcattttct acataaaaag acctgagaat ctgatggtaa
      661 aaaaagtaaa gggcttagta accggagtac tggaaggcat cgctactatt agcaaactgg
      721 aaagaccgtg gttgttcttg ttttatacgg tggcagtatg gtttttgtac tacattatgt
      781 tgtatctctg tattccggca ttcggaccta cccgacatct cggaatgcag gcagcactca
      841 ttgtgtttgt cttcggtgca ttcggatttc tggtaccaag tcctggcgga atgggcactt
      901 accactggct aatcatacag gcacttacat tatttggcat acaggctgca gacggatttt
      961 catttgccaa tatagcgtac ttcacagggc agattatatc aaacgtaggc tttgggctcc
     1021 tggcactcat attattgcct attgtaaata aaaattacca ccctaacacc aacaaatcat
     1081 gagtttccaa tcagaatata acaagaaaat caaggatctg ctcagtatga cccgacaagt
     1141 caacgtctgg agaaccaaac cagttaaaat tgtgtttacc aatggatgct ttgatatact
     1201 gcataccgga catgtacaat acctcgaaga ggcaaagtca ttgggtgaca aactcatcat
     1261 cggactaaat tcggatgcat ccgtaaaaag actgaaaggt gcagaacgac ctgtcaatca
     1321 tcaggaagac agagcgaacg tgctggcagc tcttggattt gtggacggaa tagtcatctt
     1381 tgatgaagac acaccggctg aacttattga agcattgtgc cccgatattc tcgtcaaagg
     1441 tggtgactgg ccaatagaaa agattgtagg ctctaacttt gtactcaata atggcggaca
     1501 ggtattgtcc attcctttca gagaagggca ttcgacatcc ggaacaatag aaaaaataaa
     1561 gaaactcgga taatgactac cattcgtgac ataatcggac atctcgaatc aatcgcacca
     1621 ccgtattatc aggaatcgta cgataatagt ggtctggttg taggtgaccc tgctacagcg
     1681 gtatcaggcg taatcatctg tcttgacagc accgaagaag tagtgcagga agctattgaa
     1741 aaaaaatgca accttattat tgcccatcat ccaatcattt tcggtgggct tcgtaaaata
     1801 aatggtaagg attatgtcga aaagaccatc attaaagcga ttaaacatga tattgctatt
     1861 tatgccattc acactaactt ggacaatgta ctggagaatg gtgtaaacca aaaaatttct
     1921 gaaaaacttg gccttcaaaa ctgccggata ctgcttccaa aaactcagac gcttgtaaaa
     1981 ttaaccagct tcgtgccaaa taatgcaaga gataaagttc tgaacgcggt atgttcagca
     2041 ggagcaggtc aaattggaaa ttataagaat tgcagctttt acaccactgg tacaggtagc
     2101 tttacaccga ccggtaatgc aaatcctttt cttggcgaaa tcaacaagcc agaacaggta
     2161 gaagaaagca gaatagaagt aatattaccc gaatatctta gccaagctgt gctgaatgcc
     2221 ttgaaagagg cccatccgta tgaagaagtt gcatattatc tgcaaaaatt ggaaaatact
     2281 gataccaaca ttggttcagg tatcataggc acacttgaac atcctgtagc atccgacttt
     2341 ttccttgaac agataaaaga aaaactcgga gtaacttgca tccgttatac caaactcgtg
     2401 aaatcatcgt tctgtaaggt agcagtatgt ggaggttccg ggagttttct gttgaagcat
     2461 gctattgctc agggagcaga catatttatt tctgcagatt ttaaatatca cgattttttt
     2521 gatgccaaca acaaaatcac catagcagat gttggacatt ttgaaagcga acaacatact
     2581 attcaactat tatttgacat tattaataaa aaatttagta attttgcggt tcatttgacc
     2641 caggtaatta ccaacccagt aaaatatctc tgaatatgag taaaaatgat atcagtgtat
     2701 ctgacaaact aaaaagattg tacgaactac aactcgttga ttctgaactg gctgagcttg
     2761 aaactatgaa aggagagctg cctatggaag tggctgatct cgaagatgaa atagccggcc
     2821 ttcagacaag acttcagaaa atcgaccact cggtagatga agtaaaatct gacatcaaca
     2881 aacataatac aagcatcaaa gactcggaag cactgatcgc aaaatatcag aagcagctcg
     2941 ataatgtaaa aaataatcgc gaattcgaag ctttgacaaa agaacttgag cttcaaaagc
     3001 tcgaggtgca attgcatgaa aagaaaatcc gcgaactcaa cggcggcatt caggcaaaac
     3061 ttgaactaca acaaggcaca gctgataaac ttgcccaaaa aaagggagac ctcgacctga
     3121 aaaaaagcga attggctacc atcattgcca aaactgaaaa agatgaagaa aaactcagaa
     3181 aaaaatctga aaaagcttct aaaaccattg aggacagact gcttaaggca tattcaaaaa
     3241 tcaaaaaagc ctaccacaac catcttgctg tagtaaccgt tacaagaaat gcatgtggag
     3301 gttgttttaa caagattccg ccacagctcc aacttgagat tgctctaaga aaaaagatta
     3361 ttgcatgtga acattgcggc cgcatactgg ttgatgaaca catccttgaa gctgaagtgg
     3421 cacctgtgaa tactcctgaa taatagttct taaaaataga aaagccggtt cgtcttgatc
     3481 cggctttttt gttcatcctg ttatgttcgg tattaaatca ttccttatga ttgccatgac
     3541 catggctaca gcaaacatcc aattgcaagc aattgtcagt actgattatg actttgactt
     3601 taatgaagac ataaacaatg cctacaatct ggttactgca ctaagattgg atgaagctcg
     3661 taatgctctc caccagctaa aacaaacgca accaaacaac cttgccccta tatttattga
     3721 agattatatt gactttctgt gccttatgct ttcagaagac aaagcattat tacggaatta
     3781 tcaaattaat tttaacaaca gattagcttt aatagataaa aattgtttta agcactctcc
     3841 ttattaccta ttcatcaaat ctgagatgac catgcaccgg gcagccgttg atttcagatt
     3901 tggaaacaat atccttgcat ttacagctgc attaaaggca tacaaattat tggaaacgaa
     3961 cagaactttg ttcccggatt ttgtccccaa tctcaaaaat cttggtttga tgcaaattat
     4021 tgttggctct atgcccccat ctttcaaggg tggattttct ttctttacgg gcatcagtgg
     4081 ttctgttgta atcggttctc aggatgtaaa aagagtaatg aacgaaaaaa gcagacagtc
     4141 atacatattt gcctttgagg cgaaggtgtt gtattccttc atacaattgt atatcaacga
     4201 tgaaccggat gaagcctggg cattactcag acagacaaaa gtcaattatc atgcacaacc
     4261 tttgctagcc tttatacatg caggtgcatc ctacagaagc aagcgaaatg atgaatgtat
     4321 ccggctgttg acagaaaata attatggaac tgattattac ccaatcacca tttcgaacct
     4381 catactcggc agagccaaac ttaaccgtat ggacaaggat gccaatatat ttctagagcg
     4441 ttttctcaaa caatacaaag gcactacatt tcgcaaagag gcacacctgc ttctgcattg
     4501 gtattacaca ctctttcctg atcaaaaaaa gagagaacaa cattatcagg cagttttttc
     4561 agacggcaat acgaatgctg actcagacaa tgccgccatg caggaattaa aagctaatca
     4621 tgttaacagc caggtcttcc tccgggcaag gttactatat gatggcggtt attcacagcg
     4681 tgcactgaca gagctcatac ttgcaaaatc aggcattaaa aataacccgt cgctattgct
     4741 tgagtacaac tatcgttacg caaggatact tcagcaaatg aataacatac aagatgctat
     4801 taggctctac cgacaagtca ttactaccgg caggtcacgt tcggaatatt ttgcctgtaa
     4861 tgccgcactt caaatagccg tcatctatga aaaaacaaac caacccgcca actcagaatt
     4921 ttactacaac gaatgcctgc gtataaatcc ggccacttat aaaaacagcc ttcatggtaa
     4981 ggcaaaggca ggtcttttga gattaaaaca gaaataattc tttcacaaaa ttaatttttt
     5041 atttaaataa aaatcagaat
//
LOCUS       DAOQQY010000214         5046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7583_length_5046_cov_5.812863, whole genome shotgun sequence.
ACCESSION   DAOQQY010000214 DAOQQY010000000
VERSION     DAOQQY010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7583_length_5046_cov_5.812863"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2339)
                     /locus_tag="PLE23_09765"
     CDS             complement(<1..2339)
                     /locus_tag="PLE23_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83591.1"
                     /translation="MRLKYLLGFLFFLSFQVAVFAQPCNVPPSQYNCSDVMAFDAVLC
                     NINDLDGYCTTMPDFPNPTGPSPLCNSNGGGVANNTIWFGFIAGTSSMTLQVIPANCT
                     DVGGNIGLQMGIYEEPCPPSGKEIVCTGNCVNGPYTLSSNNFVPGKTYYFWVDGCAGS
                     VCDITVDVIQGGPLMMGTIGPIKGPKKVCTGGTFTYSVDPVVGGAYYHWYLDNDPQGD
                     PTTSTEIKDITFPSAGVHKVCVDVSNFCIPESAPPAQKCIDVTVTTIVPINPKPVKIC
                     PDAEYTYAGQNYGLGDHEITFTSWQGCDSIVTLTVEPIIVPPKDLGLIYRCYGDCITV
                     EDKKGNGGVYCDNGTEEVHLESYQGCDSVVTFQLALVEIEVEIEDPDQLGCLINSTVL
                     DGSKSVNENWTNLKIKWEAFNGGKLSGPSDVLVTQTETGGKYCLTLTGTAPGGISCTD
                     SACVIVYVDPTSPQASITGDIISCYKDTVTLFGSSTAPNSDFVWTSPSGKKDTIQNLN
                     VTQPGTYTLVITAPNLCTDSETYNVVSIKANPNISSKGDTISCKNPNKTLTGTSSTPN
                     ATYKWYDSGNNVISNVNTVNVNTAGNYVFEVFDPKNGCSSTLNVPVIGDFDQPANVTA
                     SGDTFDCVKFPVKVTGNSSTTGVTYSWKGPGGFTSSLQNPDVTNAGEYELVVTGLNGC
                     KDTALAMVAADTIKPDYAIKNDTIDCFTFTGTLVASSNTPGVTYSWSGNGVTGTDPSI
                     TVNKAGIYTVVITAPNGCTRSANAEALDAKDKPVASVTVT"
     gene            complement(2464..4704)
                     /locus_tag="PLE23_09770"
     CDS             complement(2464..4704)
                     /locus_tag="PLE23_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83592.1"
                     /translation="MNNSGANGSFNNLDFSGVSPGQYVFEYTTASATAPCKDQVYTVT
                     VQVSDCNCPDITFNPAPKICQDNGTIDLINIQGNSAPGIWSIKNTPGGNNAASIQGNY
                     FISQSSDPGIYTLQYTLTNPVAGCTDSKEIQVEVVALPVATVTSTAQVCNSQGNGNFI
                     TTIDFSTLVTGGDKNGVWSDISNSGAIGSFPTLDFTGIAPGTYTFRYTTNSALNPCPE
                     VSYNVVITVKNCECPDISLTPSTDICNDIASFDLSSLLITNEAGTWSVIASPSGGKPL
                     TLNGNVLTIDHATPGSYTLQYSLSNIPPAGCPSSNTCILNILNAANAGTVLNDLSICK
                     GTKQTVSLADLLVNEDTGGKWQSDPSSAQPGTNFDATTGSLICDNLPAGTYTFDYIVS
                     GTLPCSDKTSKVNVIIHELPLVNAGQDKTINCNATSVILDPIVSTGGNIKINWTGPGI
                     SDPDILIQNVNKSGIYILEALDKNSGCKASDTLEVKMEGEPITDVIFTVKNTSCPGID
                     DGSISIDAIKGGTPDYSISLNGTNGGNSLVFPDLSSGNYILQVTDANGCSYKANITIE
                     QGEALTIDLGPDLHLLEGDKYNISYKASKSVNQIISIVWTPEVCQGCQSFEAIAAKDI
                     TYQLQIEDKYGCKANDEITIFVKKVRKVYIPNVFSPDADGINDNFIIYSDTGIKQIKS
                     LRIYNRWGGLLFDAKNFPPNDPKYGWNGKFEGKRLDNGVYVFVAEIEFTDNETAIYKG
                     DITISR"
BASE COUNT         1384 a         1044 c         1039 g         1579 t
ORIGIN      
        1 gtcactgtga ctgatgctac cggtttgtct ttagcatcca aggcctctgc attggctgat
       61 cttgtacatc cgttaggcgc agtaatgact accgtgtaaa taccggcttt gttaactgtt
      121 atacttgggt cggtacccgt aacaccattg ccactccagc tgtaggttac acccggggta
      181 ttgctacttg ccacaagtgt acctgtgaat gtaaaacaat ctatggtgtc attttttata
      241 gcataatccg gcttaatggt gtcagctgct accatagcca atgcagtatc cttacaacca
      301 ttaagtcctg taactaccaa ttcatattcg cctgcattgg ttacgtcagg gttttgaagt
      361 gatgaagtga aaccacccgg gcccttccag ctgtatgtaa caccggttgt actggaattt
      421 cctgtaactt taactgggaa tttgacacaa tcaaaagtat caccacttgc cgtgacattt
      481 gctggctggt caaaatctcc aataaccgga acgttcagtg ttgaggaaca tccgttctta
      541 ggatcgaaaa cttcaaacac ataattacct gcggtattaa cattaacagt attgacattt
      601 gaaataacat tatttcctga atcgtaccat ttatatgtag cgtttggtgt actacttgtt
      661 cctgtcaggg ttttattagg attcttacag cttattgtat caccttttga agaaatattt
      721 gggttagctt ttatagagac tacattgtag gtttcggagt ctgtacaaag attcggtgcg
      781 gtaatcacca aggtatatgt acctggttgt gttacattaa gattttggat ggtgtctttt
      841 ttacctgaag gtgaagtcca tacaaaatca gaattaggag cggtcgatga cccgaataag
      901 gtgacagtat ctttatagca tgaaatgata tctcccgtaa tggatgcctg tggggaagtc
      961 ggatcgacat agactattac gcaggcacta tcagtacagc taataccacc aggtgctgta
     1021 ccggttagtg tcagacagta ttttcctcca gtctcggtct gtgttaccag cacatcactt
     1081 ggacctgaaa gtttaccgcc attaaatgcc tcccatttta ttttgagatt agtccagttt
     1141 tcattaacgg attttgatcc atctagaact gtactgttta taaggcaacc caattggtca
     1201 ggatcctcta tttcgacttc gatttcgaca agggcaagct gaaaggtaac aacactatca
     1261 cagccctgat aggattcaag gtgaacttcc tcggtgccat tatcacaata tactcctcca
     1321 ttgccttttt tatcttctac agtaatacag tcgccatagc atcgatagat aagccccaaa
     1381 tctttagggg gcacaataat cggttctact gttagcgtaa caatactatc gcatccttgc
     1441 caggaagtaa aagtgatttc gtgatcgccc aatccataat tctggcctgc ataagtatat
     1501 tcagcatcag gacatatttt gactggtttt ggattaatgg gtacaatggt ggtaacggtt
     1561 acatctatac atttctgggc aggcggagca gattccggta tgcagaaatt cgaaacatct
     1621 acacacactt tatgtacacc ggctgagggg aaggtgatat ctttgatttc ggtacttgta
     1681 gtaggatcgc cctgtgggtc attgtcaaga taccaatggt aataagctcc acctactaca
     1741 gggtcaactg aatacgtgaa tgttccgcct gtacaaactt ttttaggtcc ttttataggg
     1801 cctattgttc ccatcatcag agggccaccc tggattacat ccacggttat gtcacatact
     1861 gatccagcgc aaccatcaac ccaaaagtaa taggtcttac ccggcacaaa attgttactg
     1921 ctaagcgtgt aagggccatt gacacaattc ccggtacaaa cgatttcttt accacttggc
     1981 ggacaaggtt cttcatagat acccatttgt aaaccgatat ttccacctac atcagtacaa
     2041 ttggcaggta ttacctgtag tgtcatactg ctagtcccgg cgatgaaacc aaaccatatc
     2101 gtgttgtttg ctacaccacc tccatttgaa ttacacagtg ggcttggacc ggtaggattc
     2161 ggaaaatcgg gcatggtagt acaataacca tccaggtcat ttatattaca caataccgca
     2221 tcaaatgcca ttacatcact acaattatac tgcgaggggg gcacattgca aggttgagca
     2281 aatacggcta cttgaaaact aagaaaaaat aaaaatccga gtaaatactt caatctcatt
     2341 aggcgttttt ttttaataga tatagttaca gcacaatacg ttgtctgtaa gcttcaaaag
     2401 taagacaatt atataagaat tgaaaatttc ttctttctca attctatttt tttagtattc
     2461 ttttcaccgt gaaatcgtaa tatctccttt atatatcgcg gtttcgttgt ctgtgaattc
     2521 tatttcggct acaaatacat aaacaccatt atcaagcctc ttaccttcaa atttgccatt
     2581 ccagccatat ttcgggtcat ttggtggaaa atttttagca tcaaaaagta aaccacccca
     2641 gcgattgtaa atccgtagcg attttatttg cttgattcct gtatctgagt aaatgatgaa
     2701 attgtcattt ataccatcgg catccggtga aaatacattt ggtatatata cttttcgaac
     2761 ttttttgaca aatatggtaa tttcatcatt agccttgcat ccatacttat cttcgatttg
     2821 taattgatat gtaatgtcct tggctgcaat agcttcaaaa ctctggcatc cctgacaaac
     2881 ttcaggtgtc caaacgattg aaatgatctg gttgacagat ttactagcct tatatgatat
     2941 attgtatttg tctccttcca gaaggtgcaa atctggtcca aggtcaatgg taagtgcttc
     3001 tccttgttca atggtgatat ttgctttgta actacagcca tttgcatctg ttacctgcaa
     3061 aatgtaattg ccggacgata aatcaggaaa aactaagcta ttacctccat tagtaccgtt
     3121 taatgatatt gaataatctg gtgtaccgcc ttttatggca tctatactta tacttccgtc
     3181 atctattccc gggcagctag tgttcttgac agtaaatatg acatcagtaa ttggttcacc
     3241 ttccattttc acttctaagg tatcagatgc tttacaaccg ctatttttgt ccaaagcttc
     3301 tagtatataa attccagact tgttcacgtt ttgtattaaa atgtcaggat cgctaatacc
     3361 gggtccggtc caattaatct taatattccc gccggtactt acaataggat ccagaatgac
     3421 actcgtagcg ttgcaattaa tggttttgtc ttgtcctgca ttcaccaatg gcagctcatg
     3481 gatgattaca ttcactttgc ttgttttgtc agaacaaggt agtgtgccac tgactatata
     3541 gtcaaatgta tatgtgcctg caggtagatt atcacaaatg agtgaacctg ttgtggcgtc
     3601 aaagttggta cccggctgag ctgaagatgg gtcagattgc cactttcctc ctgtgtcttc
     3661 gttcaccaac aaatcggcta aagaaacagt ttgttttgtc cctttgcaaa ttgataaatc
     3721 attgagtacc gtgcctgcat tggctgcatt aagtatgtta aggatacatg tattggatga
     3781 tgggcaaccc gcaggtggga tattgcttag gctatactgt aatgtgtagc tgcctggagt
     3841 tgcatggtcg attgttagta cattgccatt gagtgtcaaa ggcttgcctc cggaaggtga
     3901 tgctatgacc gaccaggtac ctgcttcatt ggtaatcaat aatgaactaa gatcaaatga
     3961 tgcaatatca ttacatatat cggtcgaagg tgttaatgaa atgtcggggc attcgcaatt
     4021 tttaactgta atgaccacat tataactcac ttcagggcaa gggtttagag cagaattggt
     4081 agtgtaacgg aatgtatatg taccgggtgc gatgcccgta aaatctagag tcgggaaact
     4141 acctatggcc ccggaattac ttatatcaga ccacacacca ttcttgtcgc cacctgttac
     4201 cagtgttgaa aaatcaatgg tggtaataaa atttccattg ccctgactat tgcagacctg
     4261 agcggtactg gtgactgtag ctaccggaag cgcaaccacc tctacctgaa tttcttttga
     4321 atccgtgcat cctgctaccg gattggtaag cgtgtactga agtgtgtata tgcccgggtc
     4381 actgctttga gagataaaat aattgccctg aatactggct gcattgtttc caccgggagt
     4441 gtttttaata gaccagattc ctggcgctga gttaccttgg atgttgatta gatcaattgt
     4501 gccgttgtcc tggcatattt taggtgcagg attaaacgta atatccggac aattgcagtc
     4561 agaaacttga accgttacgg tataaacctg atccttacaa ggtgcagttg ccgaagcggt
     4621 tgtatattca aacacatatt gccctggtga tacgcctgaa aagtcaaggt tattgaagct
     4681 gccatttgcg ccggagttat tcaaatcctt ccagttaccg gatttatcac ctgccgagat
     4741 gtagcttttc agatcaataa tgatagggta ggagccactt cctgtttggt tgcatacatt
     4801 gatggttgaa ggtaaaatgg caaaaggagg gcttacgagt gtaactgtag tagtagagga
     4861 ttcaggacaa ttgccacctg ccgggttagc cagtgtaaac tgaatggtat atgtacccgg
     4921 gtctttaccg gtgcccttga atgttgtacc taaaatagtt gccggattgg gccctccggg
     4981 tgttatattt attggctatg ttcttaataa gtgttgcaat aatgcacctg atctatatag
     5041 ttccag
//
LOCUS       DAOQQY010000215         4927 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7837_length_4927_cov_5.677956, whole genome shotgun sequence.
ACCESSION   DAOQQY010000215 DAOQQY010000000
VERSION     DAOQQY010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7837_length_4927_cov_5.677956"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="PLE23_09775"
     CDS             complement(<1..395)
                     /locus_tag="PLE23_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX83593.1"
                     /translation="MSNISLIPTEKIIERLRYENPWWVNKQIPEVYSTMARRLYFSLF
                     YPFVIEKNVKRALVLMGPRRVGKTVMLFHSIQQLLTENVNPQKVFFVGIDNPIYVHLS
                     LEDILNLCKQSLNQDNLNGCYVFFDEIQYL"
     gene            828..1475
                     /gene="pdxH"
                     /locus_tag="PLE23_09780"
     CDS             828..1475
                     /gene="pdxH"
                     /locus_tag="PLE23_09780"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HMX83594.1"
                     /translation="MTEKSIADMRVHYLSELFDMVNCSENPFEQFNTWFNQARQAQLT
                     EPNAMTLATATSSGIPSARIVLLKEFSEQGFVFFTNYNSHKGRELSENPNAALVFAWI
                     DLFRQVRIEGKVEKISPSESEKYFQSRPRGSQIGAWASPQSSVIASRSFIEEKQQQLE
                     AEYHNEAVLPLPPFWGGYLLIPQRIEFWQGRENRLHDRILFTRADAGWQRDRLAP"
     gene            complement(1524..1937)
                     /locus_tag="PLE23_09785"
     CDS             complement(1524..1937)
                     /locus_tag="PLE23_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HMX83595.1"
                     /translation="MYPIEMTRPMAEELVNAGFSSLTSPEQVEEILSKKEGTTLVVVN
                     SVCGCAAAQARPGVTLAIKSEKRPDHLITVFAGVDKEAVAQARQHMVPYPPSSPSIAL
                     FKDGELVHMLERHHIEGRSAQMIADNLVGAFEHFC"
     gene            complement(2053..3054)
                     /locus_tag="PLE23_09790"
     CDS             complement(2053..3054)
                     /locus_tag="PLE23_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMX83596.1"
                     /translation="MKAIIPVAGAGTRLRPLTYTQPKPLIPVAGKAIISFIIDDLLKE
                     GVDEFIFVIGHLGDKVKTYLQKQYPDMKMSFVVQQERRGLGHAIWLSGNLFKDESEII
                     IFLGDTILDVDLKALFSSPFSTLLVKKVQDPRTFGVVEIDEEGFATRVVEKPAIPRSN
                     LAIVGLYRIKEVTALLSALDWLIDNDHRTYNEFQLTDALEKMIEGGIRFNTMFVNNWF
                     DCGKKEILLDTNSTLLEKEDYIPVIASEPVDSIILQPVSIGKNCQISNSIIGPYVTIG
                     DNARINTSIVKDSIIGNDARLDEVMLYRSIIGNDTGVRGLKQSLNIGDNTEIDFGVH"
     gene            complement(3130..4371)
                     /locus_tag="PLE23_09795"
     CDS             complement(3130..4371)
                     /locus_tag="PLE23_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HMX83597.1"
                     /translation="MNVSIITIGDEILIGQVIDTNSAWMAGLLNEAGFAVREILSVAD
                     ENEEIKDALQRSLSKSHVVLVTGGLGPTDDDLTMEALCGYFGVEAVFHEESFERIKKM
                     FGKRNIVVTNSHRAQCHLPANATVLNNDMGTAPGMWIEHDGKVLVSMPGVPYEMKHLM
                     EERVMPRLRALYAGPPVKHLTIRTAGTGESVLEEKLRDVTTEMPKEVKLAFLPDIGQV
                     RLRYSVHGLPVEETDTILARLKTQTLDRIGEYVYAAEDISLEEWLGTVLKERNLKLVT
                     CESCTGGFLAHLITSIAGSSDYFLGSIVSYSNELKMQLLHVPSTVLEQHGAVSRETAE
                     AMAKGGLQVTGADIAVSITGIAGPGGGTTDKPVGTVWIAAASGNKVISEKFLFTKDRK
                     RNIQYSAVNAMVMLKNLIQSI"
     gene            complement(4368..>4927)
                     /locus_tag="PLE23_09800"
     CDS             complement(4368..>4927)
                     /locus_tag="PLE23_09800"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMX83598.1"
                     /translation="IIGYPVKLKVGEQFLLSMTAALAVMRFLLQYTQKKIQIKWPNDI
                     LLDNKKICGILINNSLQGMYITSSVTGMGVNINQTAFEDGLPNASSLAIAEGKEFALD
                     ELSAELYQCYEYYYQWLNTGMHELLRSEYHANLFGIGAINRFEKKDESVFSAKVKGVD
                     IDGRLMLSIGNRIEKFDIREIKLLL"
BASE COUNT         1335 a         1121 c          938 g         1533 t
ORIGIN      
        1 aggtattgta tttcgtcaaa aaaaacataa cagccattta gattgtcttg attcagagat
       61 tgtttacaca aatttaaaat gtcttctaaa cttaagtgaa cgtaaatagg gttgtctatg
      121 cctacaaaaa atactttttg cggatttaca ttttcagtca ataattgttg gatgctgtga
      181 aataacatta cagttttccc aacacgtctt ggccccataa gaaccaatgc tctttttaca
      241 tttttttcaa ttacaaatgg ataaaataag ctaaaatata gccgccttgc cattgtgctg
      301 tacacttcag gtatttgttt gttaacccac caaggatttt catatcgtaa gcgttctatt
      361 attttttctg ttggaataag acttatattg ctcataaaat catttatttt tgcaaatata
      421 gtataaataa gcagaactaa atctgtttaa ttaatgattt tttgttatta taagcgtttt
      481 agtctcttga aactcgtcta ttttatctaa gtcaagtaag tcagtactgg gatggatagt
      541 tttacaatga cgcacaatta atatgtaacg aaataaaccg tcagcttgtg aaaatggaag
      601 cattctttta ttaattaggc ttaatggaca aaaataatgc taaaaacgct caaaaggctt
      661 attatcatta gctttgaagc cactcaaaac ttacgcaata accaagcctt aatgccgtac
      721 aaatctgtct atcgcttttg atattcccga ccatttaccc gaccacctgc ttatgcctgc
      781 cattaatatt atttgtagta cttttgtgtt attaatcagt taggtctatg acagaaaagt
      841 ctattgccga tatgcgcgtg cattatttgt ccgaattatt cgatatggtt aactgctccg
      901 aaaacccgtt cgaacagttt aatacctggt ttaatcaggc gcggcaggca cagcttacgg
      961 aaccaaacgc tatgacactt gctactgcta catcatccgg cataccgagt gccagaatcg
     1021 tgctgctaaa ggaattcagc gaacaaggct ttgtgttctt tactaattat aacagccata
     1081 aaggtcgtga actttcagaa aatccaaatg cagccctagt atttgcctgg atcgacctat
     1141 tccggcaagt aagaatcgaa ggaaaagtag aaaaaatcag cccttccgaa tcagaaaaat
     1201 atttccagtc acgacctcga ggcagccaga taggtgcctg ggcttctccg caaagttcag
     1261 tcatcgcttc acgaagcttt atcgaagaaa agcaacaaca actcgaagca gaataccaca
     1321 atgaagcagt attgccatta ccgccattct ggggtggcta cttgcttatt ccgcaaagaa
     1381 tcgagttttg gcaaggtcgc gagaaccgtc tgcatgaccg tatcctgttc acacgggcag
     1441 atgcaggctg gcaacgcgac agactggcac cttaagctta aacaaagaaa cgtttgtcca
     1501 ttgaaacttt aaccggcaag ggtttagcaa aaatgctcaa atgccccaac aaggttatct
     1561 gcaatcatct gtgcagaccg accttcgata tggtgtcttt cgagcatgtg aaccagctca
     1621 ccgtccttaa ataaagctat acttggtgat gaaggtggat acggcaccat gtgctgacgt
     1681 gcctgtgcaa ccgcctcctt gtcaacacct gcaaatactg ttatcagatg atcgggtctt
     1741 ttttctgact taattgcgag tgtcacaccc ggccttgcct gtgctgctgc acatccacat
     1801 acagaattaa cgactaccag tgtagttcct tccttttttg acagaatttc ttcaacttgc
     1861 tcaggagaag taagtgaact gaaaccggca tttaccaatt cttcagccat cggtctggtc
     1921 atttctattg gatacataat cttggttgat ttaaaatatt tgttttacaa aattgaatga
     1981 atgtgaatgt accaacaagc tggttgtact tttggttcaa attaatacat ataatcttta
     2041 catgattttt tatcagtgta cgccaaagtc gatttcggtg ttgtctccta tattcagact
     2101 ttgcttaagg cctcgcacac ctgtgtcatt ccctatgata gaccgataca gcatgacttc
     2161 gtcaagccgg gcatcgttcc ctattatgga atctttcact attgaagtat tgatgcgggc
     2221 attatcccca atggttacat acgggccgat tatcgaattg ctgatctggc agtttttccc
     2281 tatgcttacc ggttgtagaa taatcgaatc aactggctca cttgctataa caggtatata
     2341 atcttctttt tcaagcaatg tagaattggt atccagcaat atttccttct ttccgcaatc
     2401 aaaccaattg ttaacgaaca tagtattgaa ccgaatgccg ccttctatca ttttttcgag
     2461 cgcatctgtc agctgaaact cattgtaggt gcggtggtca ttgtctatta gccagtcgag
     2521 tgcactcagc agggcggtta cttctttaat cctgtataag cctacaatgg caagattcga
     2581 tcgcggaata gccggtttct ctactactcg ggtagcgaac ccttcttcat ctatttctac
     2641 cacaccaaat gtgcgtggat cttgtacctt ttttaccagc aaggtcgaaa atggcgagct
     2701 gaatagtgct ttgagatcga catccaatat tgtatcaccc aggaaaatga ttatttcgct
     2761 ttcatcttta aacagattgc ccgacaacca tatagcatgg cctaggcctc tacgttcctg
     2821 ctgtacaaca aatgacattt tcatatcagg atattgtttc tgcaaataag tcttaacctt
     2881 atcgccgagg tgcccaatca cgaaaataaa ttcatcaaca ccttccttaa gcagatcgtc
     2941 tatgataaaa ctaatgatgg ccttgcctgc tactggaatc agtggcttgg gttgtgtata
     3001 agtcaggggg cggagtcgtg tgcctgctcc ggctacagga atgatagctt tcataatgct
     3061 tcatgttatt gtttacaaag gtaaaatatt tatacaactg acggccaaag tgtctgcatt
     3121 tttgaatgtt cagatgcttt gaatcagatt tttcaacatt accatagcat ttacggcact
     3181 gtattgtatg ttgcgctttc tgtcttttgt aaacaggaat ttttcagaaa tgaccttgtt
     3241 tccggaagca gctgctatcc atactgtacc taccggtttg tcggtagtac ctcctccggg
     3301 gccggctatc ccggttatac taacggctat gtctgctccg gtcacctgta gtcctccctt
     3361 tgccatggct tctgcagttt cacggcttac tgcgccgtgc tgctccaaaa cagttgatgg
     3421 aacatgtaac agttgcatct ttagttcgtt cgaatagctc acgatgctac ccagaaaata
     3481 gtcggacgaa cctgctatac tggtaatcaa atgtgcgaga aaaccacctg tacagctttc
     3541 gcatgttacg agcttcagat ttcgctcctt taatactgta ccgagccatt cttcaagcga
     3601 aatgtcttct gctgcataga catattcgcc gatgcggtca agagtttgtg tttttagtcg
     3661 ggcgagtatg gtatctgttt cttcaacagg cagcccatgt acagaatacc gtagccgcac
     3721 ttgaccaata tccggcaaga atgcaagttt tacttccttt ggcatttctg ttgtcacatc
     3781 gcgcagtttt tcctctaaaa ccgactcacc tgtacctgct gtacgtatgg taaggtgctt
     3841 caccggaggc cctgcatata atgcgcgcag acgaggcatt accctttctt ccatcaggtg
     3901 tttcatttcg tatggtacac ccggcatcga taccaatacc ttaccgtcgt gttcaatcca
     3961 cataccggga gcagtaccca tgtcattgtt caatacggtg gcattagcag gcagatgaca
     4021 ttgggcccta tgcgagttgg tgacaacaat gtttctcttg ccgaacatct tctttattct
     4081 ttcaaaactt tcttcatgaa agacagcctc cacaccaaag tatccacaca atgcttccat
     4141 ggtaaggtcg tcatccgttg ggccgaggcc tcctgtcacc agtactacat ggctcttgct
     4201 taagctcctt tgcagagcat cctttatttc ttcgttttca tctgctacag atagtatttc
     4261 ccttacagca aaacctgctt cgttcagtaa tccggccatc catgcactat tggtgtcaat
     4321 gacctggcct atcagtattt cgtcacctat ggtaatgatg cttacgttca taacagcaat
     4381 tttatttctc ttatgtcaaa tttttctatt ctgtttccaa tgctgagcat gagccgaccg
     4441 tctatatcta cgcctttcac tttagctgaa aaaacactct cgtctttttt ttcaaaacgg
     4501 tttatcgcac ctataccaaa cagattggcg tgatattcac tccgcaataa ttcgtgcatc
     4561 cccgtattca gccattgata gtagtattca taacattggt acaattctgc cgacaactca
     4621 tccagtgcga attctttacc ttccgcaatg gcaagtgaac ttgcattggg cagcccgtct
     4681 tcgaacgctg tctgattgat attaacaccc ataccggtga cacttgacgt gatgtacata
     4741 ccctgcagac tgttgttgat gaggataccg cagatcttct tgttgtcgag caaaatatcg
     4801 ttcggccatt taatctgaat tttcttttgt gtgtattgta gtaaaaaccg cattaccgca
     4861 agagcagccg tcatactcag caaaaactgt tctccaactt tgagctttac aggatagcca
     4921 attatac
//
LOCUS       DAOQQY010000216         4925 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7843_length_4925_cov_4.591992, whole genome shotgun sequence.
ACCESSION   DAOQQY010000216 DAOQQY010000000
VERSION     DAOQQY010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7843_length_4925_cov_4.591992"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="PLE23_09805"
     CDS             complement(<1..708)
                     /locus_tag="PLE23_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMX83599.1"
                     /translation="MEFIQNYINGELVPPTGGCYIDNYEPATGKVYSQIPDSDEEDVN
                     QAVDAANKAYEGWKNLTIEQRHDYLIRISELISQRSDEFALAESKDNGKPYKLAQRVD
                     IPRAVSNFKFYATAIMNWSSEANHIAEKAINYTLRQPLGAVGCISPWNLPLYLFSWKI
                     APALATGNTVVAKPSEITPYTAFLLSKICIEAGLPPGVLNIVHGLGTKAGEAIIQHKG
                     IKAISFTGGTKTGAHIAS"
     gene            complement(717..1826)
                     /locus_tag="PLE23_09810"
     CDS             complement(717..1826)
                     /locus_tag="PLE23_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HMX83600.1"
                     /translation="MSEIKKKYESVHYTDYLQLKKVLDAQHLRSPEHGEAAHDEMLFI
                     IIHQVYELWFKEVIHDLSSIMEMFRNDNVDENNIGTAVHRLNRITEILQVLVKQIDVL
                     ETITPLDFLDFRNYLFPASGFQSFQFRKVEVALGLKEKYRETYNNIPYATVFSEQESK
                     ELHDLENADSMLQLIEKWLERTPFLHRNDFLFLEAYKKAVDNMLDKERQAIDATDYLT
                     AAEKQMRMNMLGSTDTFFASVLDENKHNEMLNKGALKLSYKATLGALFINLYRDEPIL
                     QMPFQLLSKLVDIDELLTTWRYRHAQMVLRIIGRKIGTGGSSGFDYLLNTAIKHHIFF
                     DLHNISTLMIPRSELPVLPDHLKKQLSFYFTTGNK"
     gene            complement(1830..2246)
                     /locus_tag="PLE23_09815"
     CDS             complement(1830..2246)
                     /locus_tag="PLE23_09815"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020566693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMX83601.1"
                     /translation="MESKVVSGKATPRGKFPHVKIAGDFIFVSGTSSRRPDNSFEGVE
                     VDEYGTTRLDIRKQTKAVIENIRDILQSVGADLNDIVDVSSYLVNMNDFGGYNEVYGA
                     YFDFDGPTRTTVAVHQLPHPHLLIEIKVTAYKPKTK"
     gene            complement(2448..3353)
                     /gene="dapA"
                     /locus_tag="PLE23_09820"
     CDS             complement(2448..3353)
                     /gene="dapA"
                     /locus_tag="PLE23_09820"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMX83602.1"
                     /translation="MSQLFRGLGVALITPFKQDGEIDYDALERLIEHTIKGGVDYIVS
                     LGTTGEAITLNAKECRQVLDFTIDRINGRIPIVAGHFGGNDTLGLMQRIKEFDFKKID
                     GILSSSPSYNKPSQEGIYRHYCAVAEVSPRPIIIYNVPSRTASNVEAETLIRLARQCP
                     NVVAVKDASANMIQGMKTIQDKPEHFSVLSGDDPTTLPLLGVGGEGVISVIANLFPKE
                     FAGVVHNAMNGNIAEARFYNDLLQDIHPWLYKDGNPSGIKAAMHIMGMCENVLRLPLV
                     PCCEATFNGLKKELGKIADKAGTYS"
     gene            complement(3337..3891)
                     /locus_tag="PLE23_09825"
     CDS             complement(3337..3891)
                     /locus_tag="PLE23_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83603.1"
                     /translation="MKPLNQLKSFIFYKTLNTEIKRKKRTVKAINPDECQYFLILFDT
                     DDEAATQAISNYSQTLKQDGKRVVLTGFTRRPDAQTEGKNFMVVTKEDVNWYGKPVLN
                     EKTAQITNREWDCVLSLHTTPIPALDYFAATTSAATKAGFYHNNLTWLDILVDAGKGD
                     FRKSLGYLLNILKTIKSRSYEPVI"
     gene            complement(3912..>4925)
                     /locus_tag="PLE23_09830"
     CDS             complement(3912..>4925)
                     /locus_tag="PLE23_09830"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004344.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HMX83604.1"
                     /translation="KIKHTMVFLDFEKPLETLFEQLEKIKQVGEQGDLDVTPMVKELE
                     NKIAITRKEIYANLSGWQKVQLSRHPERPYTLYYISQMCKRFNELHGDRHFGDDKAIV
                     GGLANLDGQTVMILGHQKGTNTKMRQYRNFGMANPEGYRKALRLMKLAEKFGFPVITL
                     IDTPGAFPGLEAEERGQAEAIARNIYEMMVLKVPVICVVIGEGASGGAIGIGVGDRVF
                     MLENTWYSVISPESCSSILWHSWEHKETAAEALKLTAEDMFGFGLVDGIIKEPVGGAH
                     TAPEEMAKILKKQLKKELAELSELSAEERVQQRILKFSSMGRFEQLPDSKLAKLTATA
                     DKK"
BASE COUNT         1184 a         1087 c         1079 g         1575 t
ORIGIN      
        1 ggatgctatg tgagctccgg ttttggtacc tccggtaaat gaaatggcct tgatgccctt
       61 gtgctgaatg atggcttcac cggctttggt acccagaccg tgcactatat tgagcacgcc
      121 cggtggcaat cctgcttcga tgcagatttt tgacaggaga aaagccgtat aaggcgttat
      181 ttctgaaggc ttagctacga ctgtattgcc ggtagcgagg gcaggagcaa ttttccagct
      241 gaaaaggtat aagggaagat tccagggtga aatacagccg actgcaccga gtggttgccg
      301 caaggtatag ttgatggctt tttcggcaat atggtttgcc tcagagctcc agttcatgat
      361 ggctgtagca taaaatttga aattgctgac agcacgcgga atatctacac gttgtgccag
      421 tttgtaaggc tttccattgt cttttgattc ggcaagggca aattcgtctg atctttgtga
      481 aatgagttca gaaatccgta tcaggtaatc gtgcctttgt tcaatggtca agtttttcca
      541 gccttcataa gccttgttgg cggcatctac tgcctggttt acatcttcct cgtccgagtc
      601 tggaatctgc gaataaacct tgcctgtcgc cggctcataa ttgtcgatat agcacccacc
      661 tgttggtggt acaagttcgc catttatata gttttgaatg aattccatcc tagagtttac
      721 ttgttgccgg ttgtaaaata aaaactgagc tgcttcttta ggtggtcggg cagtacaggt
      781 agttctgacc tcggtatcat caaagtagag atattatgca ggtcgaagaa tatgtggtgc
      841 ttgatggccg tattcagcag ataatcgaat cctgaagagc cacctgtacc tatttttcga
      901 ccaatgatgc gcagtaccat ctgtgcatgc ctataccgcc aggtagtaag cagttcgtcg
      961 atgtccacga gcttactcag caactgaaac ggcatttgaa gaataggctc atcgcgatag
     1021 aggttgatga acagtgctcc gagtgtagct ttgtatgaca atttcagagc gcctttatta
     1081 agcatttcat tatgcttgtt ctcatcaagt accgatgcaa agaatgtatc cgtagagcca
     1141 agcatgttca ttctcatttg tttttcggct gcagtaagat aatcggttgc gtctattgcc
     1201 tgcctttctt tgtccagcat attgtccaca gctttcttgt aggcctcaag gaacaagaaa
     1261 tcgtttctgt gaaggaatgg cgttcgttcc agccattttt ctatcaattg aagcatagag
     1321 tcagcattct ccaagtcgtg aagttccttg ctttcttgtt cggaaaatac ggttgcataa
     1381 ggaatgttgt tataggtctc gcgatatttt tccttcaagc caagagctac ttctacctta
     1441 cggaactgga aactctggaa tccgcttgcc gggaaaagat aatttctgaa gtcaagaaag
     1501 tcgagaggcg tgatggtctc gagtacatct atctgtttta cgagtacctg gagtatctct
     1561 gttattctgt tcaatctgtg tacggctgta ccaatgttgt tctcatcaac attgtcgttt
     1621 ctgaacattt ccattatgct cgacaggtcg tggattactt ccttaaacca taattcatat
     1681 acttggtgta tgatgatgaa cagcatttcg tcatgggcag cctcgccatg ttcaggactg
     1741 cgcagatgtt gagcatcgag tacttttttt agctgaagat agtcggtgta atgtaccgat
     1801 tcgtattttt tctttatttc tgacatgatt tatttagttt taggtttata tgcagttact
     1861 tttatttcga tgagcagatg cgggtgaggc aattggtgta cagcgacagt ggtacgggtt
     1921 ggtccatcga aatcgaaata cgcaccatag acttcattgt agcctccgaa gtcgttcatg
     1981 tttaccaggt agctgcttac atcaacaatg tcattcaggt cagcacccac actttggagt
     2041 atgtctctta tgttttcgat gactgctttt gtctgcttgc ggatgtccag acgggtagtg
     2101 ccgtattcgt ccacttccac accttcaaag ctgttgtcgg gccttctcga actggtacct
     2161 gaaacgaaaa tgaaatcgcc ggcaattttt acgtgaggga atttgccacg aggcgtagct
     2221 tttcctgata cgactttact ctccatattt tgaatgattg gttagccaag caaatatgaa
     2281 catttttttt gacaatccgc ttaaagattg acagcataat ggcttttatg tacttttttt
     2341 tacacttatt ccattttatt ttccacaaag aaagctgcac ccaaacggtc gcttccgcca
     2401 tttttctttc ttaacgaaaa taaacaacga cagggcaggt aaaaaaatta agaatacgtg
     2461 cctgctttgt cagctatttt gccgagttct ttttttaatc cgttgaatgt ggcttcgcag
     2521 catggtacaa gcggcaggcg caatacattt tcacacattc ccatgatgtg catggctgct
     2581 ttaatgcctg aaggattgcc atctttgtaa agccatggat gtatatcctg tagcaaatcg
     2641 ttgtaaaatc tggcctcagc tatattcccg ttcatggcat tgtgtactac accagcaaac
     2701 tcttttggga aaagattggc gattacggag ataacgcctt caccgcctac accgagcaag
     2761 ggcagggtag tggggtcgtc acctgacagt accgagaaat gctccggctt gtcctgtatg
     2821 gttttcatgc cctgaatcat attggcagag gcatccttga ctgctactac attagggcat
     2881 tggcgtgcta aacgtatcag tgtctcggcc tctacatttg aggcagttcg ggaaggtaca
     2941 ttatatatga tgataggtct tggcgatact tctgccactg cacagtagtg cctgtaaatt
     3001 ccctcctgcg acggtttgtt gtaggatggc gaactcgaca ggattccgtc tattttttta
     3061 aagtcaaatt ccttaatgcg ctgcatgaga ccaagtgtat cattacctcc gaaatgtccg
     3121 gctactatcg gtatccgacc attgatacgg tctatggtga agtcaagcac ttgcctgcac
     3181 tcctttgcat tgagagtaat ggcttcgccg gtagtgccga gtgaaacgat gtagtcaacc
     3241 cctcctttga tggtatgctc tatcaatctt tccaaagcat cgtagtcgat ttctccatct
     3301 tgcttgaaag gtgtaatcag tgctacacca agtcctctaa ataactggct cataggagcg
     3361 agattttata gtttttaata tattcaataa gtatccaagc gattttcgga agtctccctt
     3421 gcccgcatca accaatatgt cgagccaagt gaggttgttg tggtaaaaac cggcttttgt
     3481 agcagcagat gttgtcgccg caaagtaatc caatgcaggt atcggtgttg tatgaaggct
     3541 gagcacacaa tcccactccc ggtttgttat ttgtgctgtt ttctcattca ataccggctt
     3601 accgtaccag tttacatcct cttttgttac caccataaag ttttttcctt cggtttgagc
     3661 atccggcctt cgggtaaaac ctgtcaaaac cactcttttg ccgtcttgtt tgagtgtctg
     3721 actataattg ctaattgcct gtgttgccgc ttcgtcatct gtatcaaaaa ggatcagaaa
     3781 atattggcat tcatccggat ttattgcttt gaccgttcgt ttctttcttt ttatttctgt
     3841 attcagtgtt ttgtagaaaa tgaacgattt taactggttt aagggcttca tggtcaatgt
     3901 ttttgtgcct tttatttttt atcagccgtt gctgttaatt ttgcaagttt gctgtcaggt
     3961 agctgttcga atcttcccat tgagctaaat ttcaggatgc gttgctgcac ccgctcttca
     4021 gctgataatt cggacaactc agccaattct ttcttcaatt gttttttaag gattttggcc
     4081 atttcttcag gtgcagtgtg tgctccacca acgggttctt tgatgatgcc gtccaccaac
     4141 ccaaagccaa acatatcttc tgccgttaat ttcagtgcct ctgcggcagt ttctttatgt
     4201 tcccaactgt gccaaagtat ggaggaacat gattccggag aaataacaga ataccaggtg
     4261 ttttcaagca taaacactct gtcaccaacg ccaatgccga tggctccacc cgaggcacct
     4321 tcgcctatga ctacacagat tacaggtacc ttcaatacca tcatttcata aatattgcgc
     4381 gcaatggctt cggcctgacc tctttcttcg gcttccaatc cgggaaaagc ccctggtgtg
     4441 tctatcagtg tgattacagg gaatccgaat ttttctgcaa gcttcatcag cctgagtgcc
     4501 ttgcggtagc cttcaggatt ggccatgccg aagttgcggt actgccgcat cttggtgttt
     4561 gtacctttct ggtgtccaag aatcatcacg gtctgtccgt caagattggc aagcccgcct
     4621 acgattgcct tatcatcacc aaaatgtctg tcgccatgca gttcattgaa ccgtttacac
     4681 atctgagaaa tataatacag tgtgtatggc ctctctgggt gtctggatag ctgaaccttc
     4741 tgccagccac tcaagttggc atatatttct ttacgtgtaa tagctatttt gttttccaac
     4801 tctttgacca taggcgtcac atcaagatca ccttgctcac ctacttgctt gatcttctct
     4861 aattgttcaa agagtgtttc aaggggcttt tcaaaatcaa gaaataccat agtatgtttt
     4921 atttt
//
LOCUS       DAOQQY010000217         4888 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7937_length_4888_cov_7.105731, whole genome shotgun sequence.
ACCESSION   DAOQQY010000217 DAOQQY010000000
VERSION     DAOQQY010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7937_length_4888_cov_7.105731"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            398..1099
                     /locus_tag="PLE23_09835"
     CDS             398..1099
                     /locus_tag="PLE23_09835"
                     /inference="COORDINATES: protein motif:HMM:NF015921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83605.1"
                     /translation="MKILVTTLGETLGDFISMTLNLGYSNGILITLLFFLIILTIQLF
                     QRKYIPIIYWLVIIGTTTLGTEISDFIDRTLHFGYALGTLLLVNCLFFTLSLWYKKYK
                     NLEVYPIADKHKEIYYWVTILFSNSLGTAFGDFLSDNIGLSYLTGAMVTGMVIIVVVL
                     LHYYSKLNHIALFWIAFIFTRPFGATLGDFLTKPHSKGGLDLGTSVASIISVIIMFIL
                     IFISSKQDKRKLEKQ"
     gene            complement(1817..2680)
                     /gene="rfbA"
                     /locus_tag="PLE23_09840"
     CDS             complement(1817..2680)
                     /gene="rfbA"
                     /locus_tag="PLE23_09840"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HMX83606.1"
                     /translation="MKGIILAGGLGTRLYPLTLAVSKQLMPVYDKPMIYYPLSTLIHS
                     GIREILIISTPHDLPKFKELLGDGSEIGCRFEYVEQHVPNGLAQAFVLGEHFIGKDSV
                     SLILGDNIFYGSGLNELLQASSNPNGGIVFAYQVADPERYGVVEFDHDFHAISIEEKP
                     TQPKSNYAVPGIYFYDNSVVEVAKNLQPSPRGEYEITDINKYYLQKGKLKVGVLGRGV
                     AWLDTGTHKSLMQAGQFIEVIEDRQGLKIGCIEEAAHQMGFIDDNQLRNLGHKYIKSG
                     YGEYLLRLLQK"
     gene            complement(2745..3122)
                     /locus_tag="PLE23_09845"
     CDS             complement(2745..3122)
                     /locus_tag="PLE23_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMX83607.1"
                     /translation="MTQILLLDHEGLMHTTLDFKLQKAGMSPVYCTPDDDLASVFNNN
                     DIAAAITDIDMDKSIVEEILDFCIKNDKHSVPLLGITNLEDEEQILYWLNRGMHDFIA
                     KPYKTSEIIIRIKKALSISEVRP"
     gene            3339..3470
                     /locus_tag="PLE23_09850"
     CDS             3339..3470
                     /locus_tag="PLE23_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83608.1"
                     /translation="MKVGTPELNACLKVPVVLNGKDTYPAKKVRILNNLELTIGASL"
     gene            complement(3553..3684)
                     /locus_tag="PLE23_09855"
     CDS             complement(3553..3684)
                     /locus_tag="PLE23_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83609.1"
                     /translation="MSNSLQDENLSQKSKVIVYFLFFLFLVIAAIVMVMKRGYQLEF"
     gene            complement(3760..>4888)
                     /locus_tag="PLE23_09860"
     CDS             complement(3760..>4888)
                     /locus_tag="PLE23_09860"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase family protein P"
                     /protein_id="HMX83610.1"
                     /translation="SRDGATLFVDEAKVPDSIKKSFTDEKISLQAYDAIEPFLKKLKK
                     PEKIWVDKSTASAWLGAAISKDLLIVDANLPKGMKAIKNKTEIKHIRQAMIQDGVSLT
                     KAFMWLEAELSKRRVTEVEVAEQLIKCRAVHKDYIGESFGAIIGYASNGAIIHYHPEQ
                     GKCASIKQEGILLIDSGGQYKCGTTDITRTLAVGKPTSEQKLHFTMVLKGNIALSRAK
                     FPKGTRGVQLDILARQFLWQHGLNYLHGTGHGVGFFLNVHEPPQGFITGLAERGTTVI
                     EPGMLTSNEPGFYVEGSHGIRIENLVLTVEDEVTPYGEFLSFETVTLAPIDRNLISSK
                     YLTPDEKAWLNNYHATVFERLSPHLTKKEAKWLKEKTKPIK"
BASE COUNT         1316 a          937 c         1074 g         1561 t
ORIGIN      
        1 aaagatgata aaccttgttg aactgatgtc tcaccgaagg tgttgcttga acatggtttt
       61 ggcgcaagtt gggcatacgg aaaaaactca tcttttgttt ttctacttgg cttcagcccc
      121 agcagggcaa tataaattaa atttagctta tgaataaaac atcggcaata ttgcatttgt
      181 cactttcagc tacgagcaga cggaattcca tttcccaacc tgcacaaagc cctgttcgtt
      241 taatgcgatt ttacttaata cccattaacc aagaaccttg aagtattata tatgatgaaa
      301 ttataatagt gttgaaactt aatgatcatt aataaaggaa ataaaacaat atgaaaatat
      361 aaacaaggta gccaaaatca ctttactctt ttggctaatg aaaattttgg taaccactct
      421 tggagaaacc ttgggtgatt ttatttcaat gacactgaat ttaggttatt caaatggaat
      481 tttgattact cttttattct ttttaataat tcttacaatt caattatttc aacgcaaata
      541 cattccgatt atttattggc ttgttataat aggcaccact acattgggga cagaaatttc
      601 tgattttatt gatagaaccc tacattttgg ttacgcatta ggaacccttc tattagtcaa
      661 ttgcttattt tttacacttt ctctttggta taagaagtat aaaaatcttg aagtatatcc
      721 aattgctgac aagcacaagg aaatctatta ttgggtaaca atactttttt caaatagttt
      781 gggcactgcc tttggtgact ttttgagcga caatattgga ttatcttact tgactggtgc
      841 aatggtaaca ggcatggtaa tcatagtggt tgtgttgcta cattattact ctaaactaaa
      901 ccacattgca ttgttctgga ttgcatttat ttttactaga ccattcggtg caacattggg
      961 agacttttta accaaaccac attcaaaagg cggtttagac cttgggacat cagtagcttc
     1021 tataatttcc gtaatcataa tgtttatact catttttata tcttctaaac aagataaaag
     1081 aaaattagaa aaacaataat ggaacaaggt cgagcaggta aaggggaatt tcaccccaag
     1141 cctctctcag aaccgcacat gcacatctcg attaatacgg ctctatatag tccagaactg
     1201 gtaaggtcga gaacactcgt agtgccagta ttcgaacaaa ttaggataac tccgagatga
     1261 taggctcttc aaatacttgc aagcattgct ccaacgtttt tttttctgca cctgcggtat
     1321 ttgttgcgga tcctttttac atgtcacttg tgcagttagc ctaaagtagt tttgtgaatg
     1381 ctgttgattg ttttcagtcg ccacattcaa attttaaacc cagataatcc attaggttat
     1441 gagatctaat atgatcggat ttctctaatc caatgaagct gggcattctc gaagatgcac
     1501 atccaatttc cttattgaaa aagttgtaag atttccttac agggaaacca aaagttttac
     1561 ctcgttatta tactcagtaa ttatctgata aacagttttt tatgtcgtta tttttctaat
     1621 ggcattcaat agagatttta cactattgaa gtttttgggt taaactactt gatgtgcatg
     1681 agggacagaa gccggcacaa agtaaggcgt atagcccgac ccgatagggg catgccctga
     1741 tgaatccgac tgatgtgtat gtggatgcta cgtgatgctt taaggcatct gtattgccgg
     1801 attgatctgg tgtgctttac ttctgaagca gtcgtaggag gtactctcca tagccgcttt
     1861 tgatgtattt gtggccgaga ttccggagtt ggttgtcgtc gatgaaaccc atctggtggg
     1921 cagcttcttc gatacagccg atcttgaggc cctggcggtc ttcgattact tctataaact
     1981 ggcctgcctg catgagcgac ttgtgtgtac cggtgtcgag ccatgcaaca cctctgccca
     2041 agacgcctac tttgagcttg cctttctgaa gatagtattt gttgatgtcg gtgatctcat
     2101 actcgcctct tggacttggt tggaggtttt tagccacttc gactacactg ttgtcgtaga
     2161 aatatatgcc aggtacggcg taattggact ttggctgagt gggtttttct tcgatactga
     2221 tagcatgaaa gtcgtggtca aactctacaa ctccgtagcg ctccgggtct gctacctgat
     2281 aggcgaaaac gatgccgcca ttggggttgg agcttgcctg gagcagctca ttgagaccgc
     2341 tgccataaaa aatattgtcg ccgagtatca acgacacact gtctttacct atgaagtgtt
     2401 cgccgagtac gaatgcctgt gcaagcccgt tgggtacgtg ttgctcaaca tactcgaacc
     2461 tgcacccgat ctccgaacca tcgccgagta gttctttgaa ttttggcagg tcgtgtggtg
     2521 tggagatgat gagtatctcg cgtatgccac tgtgtatgag tgtggacagt gggtaataga
     2581 tcatcggctt gtcgtaaacg ggcatgagct gtttgctgac agcgagtgta agtgggtaga
     2641 gtcttgtgcc gagtccgccg gcgaggatta ttcctttcat cgggtgttgc ttatgatgtt
     2701 aaaatcagat gtgtcagttg ccggcaggtg gagccggatg gctgtcacgg tctgacttcg
     2761 gaaatagaaa gtgccttttt gattctgatg atgatctcag atgttttgta tggtttggct
     2821 ataaaatcgt gcataccgcg attgagccag tacaatatct gctcttcgtc ttcaagatta
     2881 gttataccga gaagtggtac agaatgtttg tcgtttttga tacaaaaatc gagaatttct
     2941 tcgacgatgg atttatccat atcgatatcg gtgatggcag ctgctatgtc gttattgtta
     3001 aatacggagg cgaggtcgtc atcgggggta caataaaccg gcgacatacc ggctttttgg
     3061 agcttgaaat caagtgtggt gtgcatcaac ccttcgtggt ccaggagtag tatttgtgtc
     3121 atacggtatc tgaggtattt tgtaagaagc acaaaggtaa tgaaagaagt atggtcagcc
     3181 aaattttcgg aatgattcgg gttgtaggaa gtgcaaatat atttttcgtg taatgaagat
     3241 gtatattcgc ccaacacgaa aatcaatatc ttgaattggt cgattgccgg aaaaacacga
     3301 tggaaatatt ccggtagcag caaatacaat tcagccgaat gaaagttgga actcccgagt
     3361 taaatgcatg tttaaaagtt cctgtggtat tgaacggtaa agatacctac ccggctaaga
     3421 aagtacggat attaaataat cttgaactta ctatcggagc aagcttataa aaatggaaca
     3481 aactcacaat aggaaagcta ttgcaaataa taatatttac gccggaaata aaaaaggatg
     3541 aaagacggct gatcagaact cgagctgata tcctcttttc atgaccataa caatggctgc
     3601 aataaccaga aacagaaaga ataagaagta aacaataact ttagatttct ggctgagatt
     3661 ttcgtcctga aggctgttac tcatgataat tattatttat taaatcaatt gaaatacaaa
     3721 ttaaaagtaa aatttgcttt tatgaaaatc tttggctgat tatttaatgg gttttgtttt
     3781 ttctttcagc catttggctt cttttttggt caaatgtggt gataaacgct caaagactgt
     3841 tgcatgataa ttattgagcc aggctttctc atcgggtgtc aggtatttgc tgctgatgag
     3901 attcctgtct atgggtgcca atgtaacagt ttcgaagctc agaaattctc cgtatggtgt
     3961 tacttcgtct tcaacagtca gcaccaggtt ttcgattctg atgccatggc taccttcaac
     4021 ataaaaccct ggctcgttgg aggtgagcat tcctggttcg atcactgtgg taccgcgttc
     4081 tgccagccca gtgatgaaac cctgaggtgg ttcgtgtaca ttgagaaaga agcctacacc
     4141 atgtccggta ccgtgcagat agttgaggcc atgttgccac aagaactgcc gggcaaggat
     4201 atcgagctgt acacctcttg tacctttcgg gaactttgct cttgacaggg cgatgttgcc
     4261 cttgagtacc atggtgaaat ggagtttttg ctcgcttgtc ggttttccta cagcaagtgt
     4321 ccgggtaatg tcggtggtac cgcacttgta ctgacctccg ctgtcgatga gtagtatccc
     4381 ctcttgttta atgctggcac attttccttg ttcgggatgg tagtgtatga ttgccccatt
     4441 ggaagcataa ccaatgatgg ctccgaagct ctctcctata taatctttat gaacagcgcg
     4501 gcatttgatg agttgttcgg ctacttctac ttctgtcacc cggcgttttg agagttctgc
     4561 ttcgagccac ataaaggcct tggtgagcga cacaccatcc tgtatcattg cttgtctgat
     4621 gtgtttgatt tcggttttat tcttgatggc tttcatgcct tttggcaagt tggcatcaac
     4681 tataagcaga tctttgctga tggctgcacc taaccatgca cttgcagttg atttgtcaac
     4741 ccagattttc tctggttttt taagtttttt gaggaatggt tctattgcat cataagcttg
     4801 taagctgatt ttttcgtcag tgaagctttt cttaatggaa tcgggtactt ttgcttcgtc
     4861 aacaaacaac gtagctccat cacggctg
//
LOCUS       DAOQQY010000218         4886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_7943_length_4886_cov_8.201408, whole genome shotgun sequence.
ACCESSION   DAOQQY010000218 DAOQQY010000000
VERSION     DAOQQY010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7943_length_4886_cov_8.201408"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLE23_09865"
     CDS             <1..371
                     /locus_tag="PLE23_09865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83611.1"
                     /translation="FVAGQAVYFARAVLKANDYNFVYDYPDSPESCIEPRESKIVQKA
                     EKLIKIYPNPSSTGVHIESDEEIVRVFIVNSQGDISYHKQTADIRFIKYDNLPDGLYV
                     LKLITKSGKVELHKLIKQEN"
     gene            376..681
                     /locus_tag="PLE23_09870"
     CDS             376..681
                     /locus_tag="PLE23_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83612.1"
                     /translation="MKNILLICLMFCAWLSPAQSLYNNYFLSSLCCDSSLFWNEFWHT
                     PLIYKFSTGKSTYKRLNVDPPRTYLANEDGELQFVSNGCKIMDAEGNLVSNKFGLLE"
     gene            931..2013
                     /locus_tag="PLE23_09875"
     CDS             931..2013
                     /locus_tag="PLE23_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83613.1"
                     /translation="MIYLPESFSGGIRLVRHADGESWWLIVPLTTPGSFLSVHISPDG
                     LPMEQHLSVSDYMYSTFLLGEPKRPTYALKLNISGDGKKLAALDVVRGNIAMYEIDRC
                     TGHIYNPKKVYEFMVFDSTGHYFPLSFAGLVFSPNSRYLYAGMWKEELQFDTEADDIE
                     ATKYVVAKEYFYPITFDTTGIKVPEHAFMPNINFYEQQLTPDGRIIVIGANDHGTNKW
                     YAVIEDPDQGGAACNINLFGFDSHYAPLGDMCANNIPNFDLGPLPAGSCKTGTETPDI
                     TVKNFDGSLYIDIGNIPLGVKKACLKLYDILGRLLYYDCVDIIPYQDYSKEIAILDKY
                     LPPACYAAAVEFDTGGFVSKKVMVMK"
     gene            2288..2965
                     /locus_tag="PLE23_09880"
     CDS             2288..2965
                     /locus_tag="PLE23_09880"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HMX83614.1"
                     /translation="MKKYFYYILILVFMLGAQSTFAQKGTEKSDPKATALLNKLKKQY
                     NSYKTVQADFMIETEVPEKPKDTKKGQLFQAGKKYRMVLPELTSFSDAKTVWTYIKKN
                     KEIQVTNYGDKESTPFISPQELLTIYERKDHLYAISGDAVIQGKNCTEIEFKPVSSKL
                     DYFKIRLSVDKKTMQLLQAKVFYKDGVRYTLLMTKFTPNQTLDNKYFTYRASDFPGIP
                     VEDLRID"
     gene            3133..3621
                     /gene="rimP"
                     /locus_tag="PLE23_09885"
     CDS             3133..3621
                     /gene="rimP"
                     /locus_tag="PLE23_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HMX83615.1"
                     /translation="MMTIEQHILPLLESKFTEEGFEDCFLVEIVYKEAQNKLEVYIDA
                     DSGLSFDRCQKLSRYLEAFIDENNWLGEQYILEVSSPGISRPLSFIRQYKKNIGRKLE
                     ITLNDDSKYEGTLIAANDDDVITLHFITKRKEGKKNIKEEVNKEIKLEDIKKSIVKIS
                     FS"
     gene            3625..4866
                     /gene="nusA"
                     /locus_tag="PLE23_09890"
     CDS             3625..4866
                     /gene="nusA"
                     /locus_tag="PLE23_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMX83616.1"
                     /translation="MMNLAEIFSEYKDGKNIDRPTMVRVLEDFFRTLIKKKYGTDDNF
                     DVIVNTTKGDLEIWRVRQIVPDGEVTDEKSQISVSEAQGIDEAYDVGEDCYEQLTLEE
                     FGRRAILAARQALMSRIMELEKDDVYKRYVDRVGDLVTCEVNQIMKRELLVLDDATGT
                     ELLLPRTEMIKGDFYRKGDMVKCVIKSVDMRNNSPVITVSRTDNDFLSRLLEQEVPEI
                     EDGLIVIKKIVRMPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDIV
                     NFTNNLTLYIQRALTPAKISNIQINQENMTASVFLDPDQVSLAIGKGGSNIKLASKLT
                     GYELDVYRNTNESEVEDVDLDEFADEIEGWIIDSLKNIGCDTAKSVLDLSREELLRRT
                     DLEEETVDEVLAILAAEFEEE"
BASE COUNT         1658 a          902 c         1020 g         1306 t
ORIGIN      
        1 cgtttgtcgc tggtcaggcg gtttattttg cgagggcggt attaaaggca aatgattaca
       61 attttgttta tgattatcct gacagccctg aatcatgtat tgaaccaaga gaatcaaaga
      121 ttgtacagaa agctgaaaag ctcataaaga tataccctaa cccgtcaagc accggtgtgc
      181 atatagaaag tgatgaggag atagtaagag tatttatcgt caattcgcag ggagatataa
      241 gctaccacaa acaaactgct gatatcagat tcattaagta tgataatctg ccggacggat
      301 tgtatgtgct aaaactaatt accaaatcgg gaaaagtaga attacacaaa ttaatcaaac
      361 aagaaaactg aggccatgaa aaatatcttg cttatatgcc tgatgttctg tgcatggctg
      421 agccctgcac aatctctgta taataattat tttctctcaa gtctttgttg tgattccagt
      481 ttgttctgga atgaattttg gcacacgcca ctgatttata aatttagtac agggaaatca
      541 acatacaaaa ggttaaatgt agatccaccc agaacatatt tagccaatga agacggtgag
      601 cttcagtttg tatcaaacgg atgtaaaatc atggatgcag aagggaattt ggtatctaat
      661 aaattcggat tgctggaata attaaaagat agaacatgcc tgcaaaaaat aaatccggac
      721 ggcacaattt attctaccca aataggacag gcattagaaa acgcaacatc aattccaaat
      781 aagagtagta aaaatgattt tactatattt cgttcggtct ggaatcgatg ggattatgaa
      841 agcctggacc ctgagtttaa tagtgcggtc atttatagca gaatcaataa gcaaaatgaa
      901 agccagcaat acgaggtgta tgatgagggt gtgatttatc ttcctgagtc attttcagga
      961 ggtattaggt tagtcaggca cgctgatggt gagtcttggt ggctgatcgt tcctttgact
     1021 acaccgggca gctttctatc tgtgcatata tctcccgacg gcttaccgat ggaacaacac
     1081 ctgtctgtat cggattacat gtactctact tttttgttgg gtgaacctaa gagaccaacc
     1141 tatgcactca aacttaatat ctcgggtgac gggaaaaagc tggctgcctt agacgttgtt
     1201 agaggcaaca ttgctatgta cgaaatagat cgctgtaccg gccatatcta taatcctaaa
     1261 aaagtatatg agttcatggt gtttgattcc accggacatt atttcccatt atcttttgca
     1321 ggtctggtct tttcacccaa ctcgagatat ctgtacgccg gcatgtggaa agaagagctc
     1381 cagttcgata ccgaagcgga tgatattgaa gcaaccaagt atgtcgtagc taaagaatat
     1441 ttctatccaa tcacatttga caccaccggt ataaaagtac ctgagcatgc gttcatgcct
     1501 aatattaact tttatgagca gcagttgacg cccgacggca gaatcatcgt aattggtgca
     1561 aatgatcatg gcaccaataa atggtatgcg gtaatagaag accctgatca gggtggcgca
     1621 gcctgtaata tcaatttatt cggttttgat agccactatg ctcctttggg cgacatgtgt
     1681 gccaacaaca tacccaattt cgatctgggg cctctgcctg cgggtagttg taagaccggg
     1741 actgagacac cggacataac tgtcaaaaac tttgacggaa gcttatatat cgatattggc
     1801 aatattcctt taggtgtcaa aaaggcctgc ttaaagctat atgacatact cgggcgcctg
     1861 ctatactacg attgtgtgga catcatacca tatcaggact acagcaagga gatagcaata
     1921 ttggataaat acctgccacc tgcctgctat gccgccgcag tagagtttga taccggcggc
     1981 tttgtctcaa agaaagtgat ggttatgaag tgaggtatca agaagccttg gatacataca
     2041 agctttattc aacccggaat ttcacagcaa gaatgtaaac aaaaataatt attacaatgg
     2101 atggacaatg attttcaaac ttttccataa cgttactttt ttgagtgtaa cgatatttta
     2161 ggttgtgata ctaacgcgga ttctgggttt aacacacact ttaaacaata tttataaaat
     2221 tttcagcttt aacattctgt tatagttcta ttgctttaat attgcgttct attaccaaac
     2281 ccagctcatg aagaaatatt tttattacat acttattctg gtattcatgc ttggtgcaca
     2341 atcaacattc gcacagaaag gtacagaaaa atcagatccg aaagctactg cattgctcaa
     2401 caagctcaag aagcaataca acagctacaa gactgtacaa gccgacttta tgattgaaac
     2461 cgaagtaccg gaaaaaccta aggatacaaa aaaaggacaa ctttttcaag caggtaaaaa
     2521 gtacagaatg gtgttgcctg agctaacttc attcagcgat gccaaaacgg tgtggaccta
     2581 catcaaaaag aataaggaaa tacaggtcac gaactacggt gacaaggaaa gtacaccatt
     2641 tatttcacca caagaactcc ttacaatcta tgagcgtaaa gaccacttgt atgccatttc
     2701 gggtgatgcc gtcatccaag gtaagaattg tacagaaata gaattcaaac cggtttcatc
     2761 aaaactggat tatttcaaaa tccgcctttc tgtagataag aaaacgatgc aactactcca
     2821 agccaaagtg ttttacaaag atggtgtcag gtacaccctc ctcatgacaa aattcactcc
     2881 caatcagact ttagacaata aatattttac ttaccgggcc agtgattttc cgggcatacc
     2941 tgtcgaagac cttagaatag actgatgcga aatgaattac ccgagtcggg ggcaggctat
     3001 cttaaatatt ttttggctaa caatgaatat tttattaaaa taaatacaca gttagacaag
     3061 ttttcgtaac tttgtgccag taaaacattg ctaaaagtgg ggaacggtaa agttccccat
     3121 ttttattttc tgatgatgac gatagaacaa catattttac cacttttaga gagcaaattc
     3181 acagaagaag ggtttgaaga ttgctttttg gtagaaattg tatataaaga agctcaaaat
     3241 aagcttgaag tatatataga tgcagatagt gggcttagct ttgacagatg ccagaaactg
     3301 agcagatatc ttgaagcttt catagatgaa aacaactggc ttggtgaaca gtatattctt
     3361 gaagtgtcat cgccgggtat ttctcgtccg cttagtttta tcaggcaata caaaaaaaac
     3421 ataggacgaa aactggaaat aacactcaat gacgacagca aatatgaagg aacattaata
     3481 gctgcaaatg acgatgatgt tataaccctg catttcatca ctaaaagaaa agaaggtaaa
     3541 aagaatataa aagaagaagt aaataaagaa ataaaattag aagacataaa aaaatctatt
     3601 gttaaaattt cattttcata agccatgatg aatttagctg aaatcttttc ggaatataaa
     3661 gacggaaaga atattgacag accaacgatg gtcagagtat tggaagattt cttcagaact
     3721 ctgattaaga aaaaatacgg taccgatgac aacttcgatg ttattgtcaa taccaccaaa
     3781 ggtgaccttg agatctggcg tgtaagacag atagtgcctg acggagaggt gacagacgaa
     3841 aaatcacaga tctctgtatc agaagcacag ggtatcgatg aagcatacga cgttggtgaa
     3901 gattgttacg aacagctcac acttgaagaa tttggccgta gagccattct tgcagccaga
     3961 caggcactca tgtcaagaat catggagctc gaaaaagacg atgtgtacaa acgctatgtt
     4021 gaccgtgtag gcgacctcgt aacgtgtgaa gtaaaccaaa tcatgaagcg cgaactactc
     4081 gtactcgatg atgctacagg tacagaactc ctgctgccaa gaacagaaat gatcaaaggt
     4141 gatttctaca gaaaaggcga catggtgaaa tgtgttatca aaagtgtaga tatgagaaat
     4201 aactctcctg ttataacagt atcccgaaca gataatgact tcctgagcag attattggaa
     4261 caagaagtac cggaaataga agatggcctt atcgtcatta agaaaattgt gcgcatgccg
     4321 ggcgaaagag ctaaagtggc tgtcgaatcg tatgacgacc gtatagatcc ggtaggcgct
     4381 tgtgtaggta tgaaaggaag ccgcatacac ggaatagtaa gagaactgcg taacgaaaac
     4441 atcgacatcg tcaattttac taataacctc actctgtata ttcagagagc tttaacaccc
     4501 gccaaaatct caaatataca gatcaatcag gaaaacatga cagcttccgt attccttgac
     4561 ccggatcagg tatcattggc tattggcaaa ggaggctcta atatcaaact tgccagcaaa
     4621 ctcaccggat acgagctcga cgtttataga aatacgaacg aaagtgaagt cgaagacgta
     4681 gatcttgatg aattcgcaga tgaaatagaa ggctggatca tagattcact taaaaatatt
     4741 ggttgtgata cagccaaaag tgtacttgac ctgagtcgcg aagaactcct cagaagaaca
     4801 gacctcgaag aagaaactgt agatgaagta ctggctattc tggcggctga gtttgaagaa
     4861 gaatgataat aaagtataag aaatgt
//
LOCUS       DAOQQY010000219         4862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8003_length_4862_cov_4.940503, whole genome shotgun sequence.
ACCESSION   DAOQQY010000219 DAOQQY010000000
VERSION     DAOQQY010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_4862_cov_4.940503"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="PLE23_09895"
     CDS             <1..1158
                     /locus_tag="PLE23_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMX83617.1"
                     /translation="LNVFENQDLDYFINQQYAKADKRYHSNLYSETPNAALMVEAVPP
                     VFSEDSQVKNGYEILNEFFQIKFSENNKLVAFGEDVGKIGDVNQGFAGLQAIFGEDRI
                     FDTGIREWTIMGQALGLAMRGFRPIAEIQYLDYLIYGLQPLTDDLATLRYRSNGIQHA
                     PAIIRSRGHRLEGIWHAGSPIGMLIHSLRGMYIMVPRNMVQAAGFYNTMLKSDDPGLI
                     IECLNGYRLKETRPDNLADFCVPLGVPEILAEGHDITLVTYGSCVRIAEAAIQQLTQY
                     NISVELIDVQTLLPFDTGHTICQSLKKTNRIVFLDEDVPGGASSYMLQQVLEVQGGYQ
                     YLDSKPVTITAHAHRPPYGSDGDYFTKPSAEDVFDVCYNLMNEYDPGNYPV"
     gene            1189..1917
                     /locus_tag="PLE23_09900"
     CDS             1189..1917
                     /locus_tag="PLE23_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HMX83618.1"
                     /translation="MPLNLVIIPTYNEKENIEKILKAVIAQSDDFDVLIVDDNSSDGT
                     ADIVKKMQTIYPDRIILIQRAGKLGLGTAYIEGFKFGLEHNYSFVYEMDADFSHNPAD
                     LQRMYEVLKSGQADVVVGSRYVKGGSVVNWPFDRRAISYGGSLYTRLITWMPVKDPTA
                     GFIGYNAKVLAAIDLQKIQFVGYAFQIQMKFEAWSLGFVIKEIPIVFKDREEGVSKMS
                     GSIVKEAAFGVLGMKIRKLFSRFS"
     gene            2165..2623
                     /locus_tag="PLE23_09905"
     CDS             2165..2623
                     /locus_tag="PLE23_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HMX83619.1"
                     /translation="MGQLLGEFQCRMDEKGRVRLPSSLLRQLNELGGYNLVINRSVDK
                     CLMLYPKVVWDKIKVEVDKLNPYVKKNREFIRYFYRGATELSPDSAERILLPKALLEY
                     AEIDRDLVLFAYNNKIEVWSQANYDALIGEEKGDDFSDIGEDILGSLNDV"
     gene            2626..3531
                     /gene="rsmH"
                     /locus_tag="PLE23_09910"
     CDS             2626..3531
                     /gene="rsmH"
                     /locus_tag="PLE23_09910"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMX83620.1"
                     /translation="MSEKNYHVPVMLNECIEYLNIDPNGIYVDVTFGGGGHSKAILKH
                     LGADGRLYAFDQDPDAATNVVKDERLIFVDGNFRELKKMLKVHGVRQVNGILADLGVS
                     SHQLDAAKRGFSYGSDEFLDMRMQPGSSLTAADVLNKHTPDDLLKVFSHYGEVRNSRT
                     LADAVVQARSQRKFKVVPDLLAVLETVMVGDRFKYLAQVFQALRIEVNDEMGALKDLL
                     MQGGEVLAPGGRFVVMSYHSLEDRLVKNFFKAGNPEGEHDKDFYGNIKKNFKILTKKP
                     VEPTLEERRSNTRSGSARLRVAEKI"
     gene            3554..3856
                     /locus_tag="PLE23_09915"
     CDS             3554..3856
                     /locus_tag="PLE23_09915"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HMX83621.1"
                     /translation="MKFKKLLEKYKISPAQAFFHLPFLGFLTLLGVLNIANGYMSIEN
                     IEEIQSLQQEIKELRWQAIDIESKMLSEAKESQISNRVSSINLEVSKTVPKTIVIP"
     gene            3872..>4862
                     /locus_tag="PLE23_09920"
     CDS             3872..>4862
                     /locus_tag="PLE23_09920"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HMX83622.1"
                     /translation="MNVKNELLSRVYLVTLIVVLAAILILVQLSKIGLAQRDKWIKMG
                     DKFSLSMEPVDAERGNILTESGATLATSLPFYELRMDTGCPGLTDNIFKENLDSLCYN
                     LWKYVDPTVSPKAWKSKLVMARHEKNRFLLLKDDIGYEVFEKVKNFPMFRLGRNIGGV
                     IGVKKSKREFPYGNLARRTIGYHKETVQPVGIEGAFNNILRGEQGMRLMQRIPGGSKI
                     PAEDFDRIAPINGNDVVITIDETIQDIAHDELYNALKHHDAAYGCAVVMEVKTGKIRA
                     MVNLEKSNGDYGEALNYALGAAVEPGSTFKTASILALLEDGYLSSVKDSVRLFK"
BASE COUNT         1508 a          902 c         1123 g         1329 t
ORIGIN      
        1 ctgaatgtct tcgaaaacca ggatctcgat tatttcatta atcaacagta cgccaaggct
       61 gataagaggt atcatagcaa cctgtacagc gaaacaccga atgctgctct aatggtagaa
      121 gcagttcctc cggtgttttc tgaagatagt caggtgaaga atggttatga aatattgaat
      181 gaattttttc aaataaaatt ttctgaaaac aacaaactgg ttgcctttgg ggaagatgta
      241 ggcaaaatag gtgatgtgaa ccaaggtttt gccgggttgc aagccatctt tggcgaagac
      301 cgtatatttg acacaggtat ccgcgaatgg actatcatgg ggcaagctct tggtctggca
      361 atgagagggt ttagacctat agccgaaata caatatctcg actacctgat atacggactg
      421 cagccgctga ccgatgacct ggctacactc agatacagaa gcaatggcat acaacatgct
      481 ccggccatca tccgctcacg cggtcatcgt cttgaaggaa tctggcatgc aggttctcca
      541 ataggtatgc tcatacatag cctacgaggt atgtacatta tggtgcctcg caatatggtc
      601 caggcggcag gtttttataa tacaatgctg aagtcagatg acccggggct gataatagaa
      661 tgcctgaacg gatacaggct aaaagaaacc aggcccgata acttggccga tttctgtgta
      721 cctcttggtg ttcccgagat attggccgaa gggcacgaca ttacactagt cacgtacggt
      781 tcttgcgtaa gaatcgcaga agcagctatt caacagctta cacaatataa tataagtgta
      841 gagttgatag atgtacagac gctgttgccg tttgataccg gacacaccat ttgccaatcg
      901 cttaaaaaga caaacagaat agtttttctg gatgaagatg tgccgggcgg tgccagttca
      961 tatatgctgc agcaggttct cgaagtacag ggcggatacc aatatctcga ttcaaagccg
     1021 gtaactatta cagcacatgc acaccgtcca ccttatggca gtgatggtga ttattttact
     1081 aagccaagtg ctgaagatgt ttttgatgta tgctacaatt taatgaatga atatgacccg
     1141 gggaactacc ctgtgtaata gattaaaaaa caacataaaa gtccgacttt gcccttaaat
     1201 ctcgtaatca taccaacata caacgaaaag gaaaatatag aaaaaatcct taaagcagtt
     1261 attgcacagt ctgatgattt tgatgtgctt atcgtagatg ataattcttc agacggcact
     1321 gccgatattg tcaaaaaaat gcaaaccatc taccctgaca ggataatcct tatacaaagg
     1381 gcaggcaaac tcggtttagg aacagcttat atcgaaggtt ttaagtttgg cctagagcat
     1441 aattatagtt ttgtgtatga aatggatgca gatttcagtc ataatccggc tgatcttcaa
     1501 cgaatgtatg aagtacttaa gtcaggtcaa gccgatgtag ttgtgggttc aaggtatgta
     1561 aaaggaggta gtgttgtcaa ctggcctttt gaccgccggg caatatcata cggtggttcg
     1621 ctttataccc ggcttatcac atggatgcct gtcaaggacc cgactgccgg atttattggt
     1681 tataatgcca aagtattggc agccattgat ttacaaaaaa tacagtttgt gggctatgca
     1741 tttcagatac aaatgaagtt cgaagcatgg tcactcgggt ttgttatcaa agaaatacca
     1801 atcgtattca aagatcgtga agaaggtgtt tcaaaaatga gcggcagcat tgtcaaagaa
     1861 gccgcatttg gtgtacttgg aatgaaaatc cgtaagttat tctccaggtt tagttaaatt
     1921 tctgtttttt ttcgtaaaaa ttacccacat cttaccactg cacttttagt ggtgagtaat
     1981 attatttatt ttcattaata ctttgattat taaccaatta tggattattt gataaaatga
     2041 tctaatccgt agattatttt atgtttcata aaaattgaat tatttttgaa aaaaagtgta
     2101 aaaaagtggg aattgttgtg aatatgtgat aattttacac ttcaaatagt agttaagtca
     2161 aaagatgggt cagctgttag gagaatttca atgccgcatg gatgaaaaag gacgggtacg
     2221 cctgccttct tctctgttgc gtcaattgaa tgaactcggc ggatacaatc tggtgattaa
     2281 cagaagtgtt gacaagtgtc ttatgttata tccaaaagtg gtttgggata agataaaggt
     2341 agaagttgac aaactgaatc cttatgttaa gaaaaaccgt gagtttatta ggtattttta
     2401 cagaggagct actgagttga gtccggatag tgctgagcgg attttgcttc caaaggcatt
     2461 gctggaatat gctgaaatag accgtgacct ggttcttttc gcttacaaca acaagatcga
     2521 agtctggtca caggccaact acgatgcgct gataggcgaa gagaaaggtg atgacttctc
     2581 cgatattggc gaagacattc tgggttcttt aaatgatgtg taaaaatgtc tgaaaagaac
     2641 taccacgtac cggtaatgct caacgaatgc attgagtatc tcaacattga cccgaacggc
     2701 atttatgtcg atgtgacatt tggaggcggt ggtcattcaa aggctatact aaaacacctt
     2761 ggtgccgatg gtaggttgta tgcttttgat caggatccgg atgcagctac caatgtggta
     2821 aaagatgagc gtttgatctt tgttgatggc aatttcagag aattgaaaaa aatgttgaaa
     2881 gtccatggag tcagacaggt taacggaata ctcgcagatt tgggtgtttc gtcacaccag
     2941 cttgatgcgg ccaaaagagg cttctcctac ggatcggatg aatttcttga catgcggatg
     3001 cagccgggct catcattgac agcagcggat gttctgaata agcacacacc tgacgatctt
     3061 ctcaaagtat ttagtcacta tggtgaggtg cgtaattcaa gaacgctcgc cgatgcagta
     3121 gttcaggcaa ggtcgcagcg aaagtttaaa gtggtacccg acctgttagc agtacttgaa
     3181 acggtaatgg taggcgaccg atttaaatac ctcgcacaag tttttcaagc attgcgcata
     3241 gaagtaaatg atgaaatggg cgcattgaaa gatttgctta tgcaaggtgg cgaagtgctg
     3301 gcgccgggtg gtaggtttgt ggtcatgagc tatcactcac tcgaagatag gcttgtgaag
     3361 aattttttca aagcaggaaa cccggaaggc gaacacgaca aggatttcta tggcaatatt
     3421 aaaaagaact ttaaaatact gaccaaaaag cctgtggaac caacccttga agaacgaaga
     3481 agtaatacaa gatcaggaag tgcaaggctg agagtcgcag aaaaaattta aatatacaat
     3541 atataaccgt aaaatgaaat ttaagaaact actggaaaaa tacaagataa gcccggctca
     3601 ggcattcttc cacttgcctt ttctcggctt tcttacctta cttggagtgc ttaacattgc
     3661 caacggctat atgtccattg aaaatattga agagatacaa tcattgcaac aagaaataaa
     3721 agagctgagg tggcaagcca tcgacattga gtcgaaaatg ctgtccgaag caaaagaatc
     3781 acaaatatca aaccgtgttt cttccattaa cctggaagtt tcaaaaacag tcccgaaaac
     3841 aattgtaatc ccataatttt ttatacaaac catgaatgta aagaatgagt tgctgtcaag
     3901 agtatatttg gtcacactaa tcgtagtttt ggcagcgatt ctcattctcg ttcagttaag
     3961 caaaatcggc ctggcacaaa gagataaatg gataaagatg ggcgataagt ttagcctgtc
     4021 aatggagcct gtggatgccg aacgaggtaa catcctcacc gaaagtggtg ctacgctggc
     4081 cacttcactt ccgttttatg agctcaggat ggataccggc tgcccgggcc ttactgacaa
     4141 tatatttaag gaaaatcttg actcgctgtg ctacaatctt tggaaatacg tcgatccgac
     4201 tgtaagccct aaagcatgga aatccaagct ggtcatggca cgtcatgaga agaaccgatt
     4261 cctcctgctc aaggacgata ttggttatga agtttttgaa aaggttaaga attttccaat
     4321 gttcaggctt ggtcgaaata taggtggagt gataggggtc aagaaaagta aacgcgagtt
     4381 tccgtatgga aatcttgctc gccgtaccat cggttatcac aaagaaaccg ttcagcctgt
     4441 aggtattgaa ggtgcattca ataatatact tagaggcgaa caagggatgc gtctgatgca
     4501 gcggataccg ggaggatcaa agattccggc agaagatttt gaccgaatcg cacctattaa
     4561 tggcaatgat gtggttatta ccatagatga aactatacag gacatagcac acgatgagct
     4621 ttacaatgct ctcaagcacc atgatgcagc ctatggctgt gctgttgtta tggaagtaaa
     4681 gacaggcaaa ataagagcta tggtgaacct tgaaaaaagt aacggagact atggcgaggc
     4741 acttaattat gcactaggtg cagcagttga acccggatct acctttaaga ctgccagcat
     4801 acttgctttg cttgaagatg gttatttgtc cagtgtcaaa gattcagtcc gactctttaa
     4861 gg
//
LOCUS       DAOQQY010000220         4847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8041_length_4847_cov_5.945743, whole genome shotgun sequence.
ACCESSION   DAOQQY010000220 DAOQQY010000000
VERSION     DAOQQY010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8041_length_4847_cov_5.945743"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..776
                     /locus_tag="PLE23_09925"
     CDS             141..776
                     /locus_tag="PLE23_09925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMX83623.1"
                     /translation="MTKEIYIIRHGETELNKLRIIQGSGIDSSLNEKGRSQARAFYDF
                     YKAEDFQFVYTSALKRTVQSVQDFLLEGIPYRSLSDINEISWGIHEGQSPNPVLTNSY
                     LEVVKKWDAEDYDAALPQAESPRELSDRLRRFVDELLANPAEKLLVCSHGRAIRCLMT
                     ILHDEPLKMMEQYDHSNTGLFKVLYKNGRFASLTLNNTDHLHTEELRSMVW"
     gene            complement(798..1982)
                     /locus_tag="PLE23_09930"
     CDS             complement(798..1982)
                     /locus_tag="PLE23_09930"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMX83624.1"
                     /translation="MYIVTLCLLSFFSFGQKPAYFYYESKVENGDGILSFMRRYHLEE
                     FSCNFNKFYELNKIKKGALLRADIPYVLPIMVYVYNGKSIRSTLKINDLEQAKRIQAY
                     NEEMVKKKIKKKSFLTDKELWVPYSEIHCSPTDVPKSSPKELLSDEPASGTSRKYPIF
                     GPKYENVSITSNKLAGQVYYIESGHGGPDPGAMAEVSGNIISEDEYAYDIALRVARLL
                     ISQGATAYIVLRDMNDGIRDEKYLELDNDEICYGGLTIPLDQKKRLQQRAKAINNLYL
                     KHKKQGVKVQRALLIHIDSRNKSKKTDVFFYHQDDIKESIRISKNMLSVFILQYAKHR
                     KGRQYEGKLSTRNLLMLRELWPPTVFVELGNIKNYNDQKRILQADNRQLLAQWLYEGL
                     IK"
     gene            2107..2757
                     /locus_tag="PLE23_09935"
     CDS             2107..2757
                     /locus_tag="PLE23_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HMX83625.1"
                     /translation="MQAIFSLLVKEFTLEWRQKHSFGALLLYVLATIFIVFSVIKNIA
                     PDVWIALFWIIVLFASANASARSFYQESGARQLFYYQLTHSLNFIFSKFLYNFILLFL
                     IGLLTWLGFIFFSKAIVRDYGLFVLTIVLGCLSFSTIFTFISAIANKTPNGSSLMAIL
                     SFPLMIPVILTLLKLSAQACRLMQDTGYVKDIIILLSINGMLIGVMIFLFPFVWRD"
     gene            2767..3843
                     /gene="ccsA"
                     /locus_tag="PLE23_09940"
     CDS             2767..3843
                     /gene="ccsA"
                     /locus_tag="PLE23_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMX83626.1"
                     /translation="MANISNTWAKFISIGKYIGAGLIVYSLISGLLVPLKPGILAVEP
                     VTLDAGKQSVIHVTGYNTHFEEGKELSAFLKFNDGTFLESANNLVRSGKKMDIAFHIP
                     YPLPFPDRVSDAVLVVNSPEDGTMIKPAAVTVRQAVLPVATEMDGVFLKDAGKLHVHD
                     AWSFPYRGILEETIRNIFYHVPLWIAMVVLFVLSVVSSLYFLKTKQEKYDEIAVATTS
                     IGILLGILGLATGAVWAKNTWGQYWSGDIKQSMTAIALLIYFAYFILRSSLEDDVKKY
                     SLSAVYNIFAFVALIPLIFVIPRLTDSLHPGNGGNPALGGEDLDNSMRLVFYPACIGW
                     ILLSLWMSDILRRIIRLERSTQND"
     gene            3845..4060
                     /locus_tag="PLE23_09945"
     CDS             3845..4060
                     /locus_tag="PLE23_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83627.1"
                     /translation="MKNKKIISLVFFILSAIRLSAASGGEDFMSNIGKIYVVIAVIVL
                     IFIGIFAMLLHIERRVKILENQKINNE"
     gene            4053..>4847
                     /locus_tag="PLE23_09950"
     CDS             4053..>4847
                     /locus_tag="PLE23_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HMX83628.1"
                     /translation="MSSHDSFFKSVERNFDKAASHTDLHKGLLEQIKVCNAIYSMRFP
                     VKIGNEYQVIEAYRVQHSHHRLPTKGGIRYSDNVNQEEVMALAALMTYKCAIVDVPFG
                     GAKGGVKINPKNYTENQLERITRRYTTELIRKNFIGPGIDVPAPDYGTGQREMAWILD
                     TYQTFKHGELDAVACVTGKPVSQNGIKGRTEATGRGVFYGVREAMSFKDDMKKLGLTP
                     GIEGKTMVVQGLGNVGSHTALISQQEGDVKIIGVAEYEGSIYSENGI"
BASE COUNT         1393 a          938 c         1057 g         1459 t
ORIGIN      
        1 agaacacaaa gtgtgaaaac aaataaggtt ttaaaatatt taaaccgtga gttcgactca
       61 caaatattgc caacaaacaa taaaaaatat ctcggttaat ctgaattttc ctaatattgt
      121 atttcaaaaa caatcataca ttgacgaagg aaatctacat catacgacac ggcgaaacag
      181 agctcaacaa attgcgcatt attcaaggaa gcgggataga cagcagcctt aatgaaaagg
      241 gtaggtcaca ggcacgcgca ttttatgatt tttataaagc tgaagatttt cagtttgttt
      301 atacttctgc actgaagcgc accgtgcaaa gtgtccaaga ctttcttctt gaaggcattc
      361 cgtacaggtc actcagtgat attaacgaaa tatcctgggg tattcacgaa gggcagtcac
      421 ccaatccggt tttgacaaat tcataccttg aggtagtaaa aaaatgggat gctgaggact
      481 acgatgctgc attgcctcaa gctgaatcac cacgtgaatt aagtgacagg ctaagacgtt
      541 ttgtagatga attgcttgcg aatcctgctg aaaagttact tgtgtgtagc catggtcgtg
      601 ctataaggtg tctcatgacc atcctgcatg atgagccatt gaaaatgatg gaacaatacg
      661 accacagcaa cacaggattg ttcaaggtgt tatataaaaa cggtcgtttt gcatccttga
      721 cactcaacaa cacagatcat ctgcatacag aagaactcag gtcaatggta tggtaacaaa
      781 cgctgaacag taatacatca tttgattagt ccttcataaa gccattgggc gagtagttgt
      841 cggttatcgg cctgtagtat tcttttctgg tcattgtagt ttttgatgtt gccaagttcg
      901 acaaatacgg taggtggcca cagttcgcgc agcataagca ggttacgcgt agagagctta
      961 ccttcatact gacggccttt gcgatgcttc gcatattgaa ggatgaatac actcagcata
     1021 tttttcgaaa tacggatcga ttccttaata tcatcctggt ggtagaagaa tacatcggtc
     1081 ttcttgcttt tatttcgcga atctatatgt atgagcaatg cccgctgtac tttaacgcct
     1141 tgttttttat gcttcagata aaggttgttg atggctttgg cacgctgctg gagccgtttt
     1201 ttttgatcaa gtggaatggt aagcccgcca tagcagattt catcattatc tagttctaga
     1261 tatttttcat ctcgtatgcc atcattcatg tctcgtagta caatataggc agtagctcct
     1321 tgcgaaataa gcagtctggc tacacgaagt gcaatgtcgt aggcatattc atcttctgag
     1381 atgatattgc cgctcacttc agccatagcg ccggggtcag ggccaccgtg tccgctttct
     1441 atgtaatata cctgacctgc aagtttgttg gaggtgatgc ttacattttc atacttcgga
     1501 ccgaaaatcg gatactttcg tgacgtacct gaagcaggct cgtcgcttaa gagttccttg
     1561 ggtgaagatt ttggtacgtc agtcgggcta cagtgaatct cagaatacgg cacccacaat
     1621 tccttatcag ttagaaaact ttttttcttt attttcttct tgaccatttc ttcattgtag
     1681 gcctgaatcc gttttgcctg ctcaagatca tttattttca gtgtagaacg aatgcttttt
     1741 ccgttataaa catagaccat aatgggcaag acataaggaa tgtcagcccg caaaagggca
     1801 cctttcttga ttttgttcag ttcatagaat ttattgaaat tgcagctgaa ctcctcgagg
     1861 tggtacctgc gcataaaaga taggatgcca tcgccatttt ccacttttga ttcgtaataa
     1921 aaatatgcag gtttttgtcc aaaactgaag aaagaaagca ggcacaaagt aactatgtac
     1981 acaagcctgt gtatcagttt ttgattccat ctatatgtag aatgagttgt caaaatatta
     2041 atattaacgg atgatttttc cacttaatta aggaagttga ctaattttgt tgccgaagat
     2101 aggattatgc aagcaatttt ttcacttttg gtcaaagaat ttacacttga gtggcgacaa
     2161 aaacacagtt ttggtgcttt gttgctctat gtgctggcta ccattttcat cgttttttca
     2221 gtcattaaga acatcgcacc cgatgtttgg attgccttat tttggattat tgtgcttttt
     2281 gcatctgcca atgcgtctgc aaggagtttt tatcaggaaa gtggtgccag gcaattattt
     2341 tattatcagt tgacgcattc gctcaatttt attttttcaa aatttttata caattttatc
     2401 cttttgttct tgattgggtt acttacgtgg cttggtttta ttttcttcag taaggctata
     2461 gtaagagact atggtttgtt tgtactcacc atcgtgttgg ggtgcctgag tttttctacc
     2521 atttttacat ttatttcggc cattgccaat aagactccaa atggttcatc attgatggct
     2581 atcctgagtt ttccgttgat gatacctgtg atacttacat tgctaaaact cagtgcacag
     2641 gcttgcagac ttatgcagga taccggttac gttaaagata ttatcatttt attgtccatc
     2701 aacgggatgc ttataggtgt catgattttt ttgtttccgt ttgtttggcg agattaatac
     2761 aagaatatgg caaacatcag caatacttgg gcaaaattca tttctatagg caaatatatc
     2821 ggcgccgggc tgatagtata ttcactcatc tccggtctgc tcgtgccact taagccgggc
     2881 atcttggcag tagagccagt aacgcttgat gcaggcaagc agtcggtcat tcatgtcaca
     2941 ggttataata cacatttcga agaaggcaaa gaattaagcg cttttttgaa attcaacgac
     3001 ggtacattcc ttgaatcagc caataatctg gtgcgttcgg gcaaaaagat ggatattgcc
     3061 tttcacatac catatccact accatttccc gacagggtat cagacgcagt tttggtcgtc
     3121 aattcgccgg aagacggaac aatgattaag cctgcagctg taacagtgag gcaggcggtc
     3181 ttgccggttg ctaccgaaat ggacggtgtt tttctaaaag atgcaggaaa gctacatgta
     3241 catgatgctt ggtcatttcc atacagaggt attcttgaag agaccatccg gaatatattt
     3301 taccacgttc cgctctggat tgctatggta gtattgttcg tgttgtcggt cgtgtcgagc
     3361 ctgtattttt taaagacaaa acaggagaaa tacgacgaaa tagcagtggc caccacttca
     3421 atcggcatct tactcggtat tctcggactg gctaccggtg cagtctgggc taaaaacacc
     3481 tggggccaat actggagtgg cgacatcaaa caaagcatga cggccatcgc actactcatt
     3541 tattttgcat actttatcct acgctcatcg ctcgaagatg acgttaaaaa atattcgttg
     3601 agtgcagttt acaatatttt tgcatttgta gcattgatac ctttgatatt tgtcattcca
     3661 aggcttacag acagtctgca tcccggcaac ggaggtaatc ctgctttggg tggagaagac
     3721 cttgacaact caatgaggct ggtcttttat ccggcatgca tcggttggat attgttatca
     3781 ttatggatga gcgatatatt acgaagaata atacggcttg aacgcagtac acaaaacgat
     3841 taatatgaaa aacaagaaga taatatcctt agtatttttt atactttcag caataagact
     3901 ttcggctgca tcaggtggtg aagattttat gagcaatatt ggtaaaatat atgtagtcat
     3961 tgcagtaatc gtattgatct tcatcggtat atttgcaatg cttttgcata tagagcgaag
     4021 agtgaaaatt ttagaaaatc aaaaaattaa taatgagtag tcacgattca tttttcaaaa
     4081 gtgtggaacg caactttgat aaggctgcat cacacaccga ccttcataaa ggtctgctgg
     4141 aacaaataaa ggtttgcaat gctatttact cgatgaggtt cccggtgaaa atcggtaacg
     4201 agtatcaggt aatagaggct taccgggtgc agcatagtca tcaccgattg ccgaccaagg
     4261 gcggtatccg ctacagcgac aatgttaacc aagaggaagt aatggctctg gcagctctga
     4321 tgacctacaa atgcgccata gtagatgtac cattcggtgg tgcaaaaggt ggtgtaaaaa
     4381 ttaatccaaa aaattacacc gaaaaccagc tcgaaagaat taccagaagg tacacgaccg
     4441 aattgatacg taagaatttc ataggcccgg gtatcgatgt gcctgctcct gactatggta
     4501 caggacaaag ggagatggca tggatacttg acacctacca gacatttaag catggcgaac
     4561 ttgacgccgt agcatgtgtc accggtaagc cggtaagcca gaatggtatc aaaggaagaa
     4621 cagaagctac aggtcggggt gtgttttatg gtgtgagaga agctatgagt ttcaaagacg
     4681 atatgaaaaa actcgggctc actcccggta tcgaaggcaa aaccatggta gtccaaggac
     4741 tcggtaatgt agggtcgcat actgcactta tctctcagca ggaaggtgat gtcaaaatca
     4801 taggcgtggc agaatacgag ggctcaatat attcggaaaa tggcatt
//
LOCUS       DAOQQY010000221         4846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8045_length_4846_cov_5.233563, whole genome shotgun sequence.
ACCESSION   DAOQQY010000221 DAOQQY010000000
VERSION     DAOQQY010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_4846_cov_5.233563"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PLE23_09955"
     CDS             complement(<1..1103)
                     /locus_tag="PLE23_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMX83629.1"
                     /translation="MHKRLLAKFQTMIRVRLLQFMYLVFKALIGIGLRLFYPNTVVHN
                     RQYLNTKGPMLLIGNHPNGMMDPINVALHSRRMVHFLAKSQLFSNWFTRWFFNTFYCI
                     PVQRSNFSTDQPIDMVRSFERCDIFLHEGGTLFVAPEGTSLNVRRILPLRTGAARIAL
                     SAESKKDFQLGLRFVVSGVTYIDAGSFWSGQFISTSAPFGIEEFEETYRKDEVTAVKL
                     VTEKIFRELKKLNIHLENDALIPDFERLLRWFNPGIEADPEQFFIKGKLLADDINRLE
                     QNNPLLIESARTHIAVVENYMHKWHLKAEQVPESIEPTSVLSFIVHLLLAVLGLPCML
                     AGLVIHFIPNFLPWLIEKIAKQHPIYIATTKFVT"
     gene            complement(1067..2134)
                     /gene="lpxK"
                     /locus_tag="PLE23_09960"
     CDS             complement(1067..2134)
                     /gene="lpxK"
                     /locus_tag="PLE23_09960"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HMX83630.1"
                     /translation="MTNNIFLKIILFPFSLIYGTVIVIRELLYKADLLRGVSFDIPVI
                     SVGNLSVGGTGKTPHIEFLIRLLSPYIQIGTLSRGYKRKTSGYRVVNEFDSSLDAGDE
                     PLQIKRKFKDVFVAVCENRVFAIPKMLITKPDLQTILLDDAFQHRQIIPGLNILLTQH
                     SRPFFKDFLLPSGSLREFPESYKRADVIIVTKCPDKLSLDDAEKFTKSLRPFSHQKVF
                     FSKFKYRRPYFWFNPSFSYNLKEDTDVLLISAIANSDYLVDFLHANVNSIEMLEFRDH
                     HYFKEEDMRAIHSLFRSMKSENKIILTTEKDAGRLELFRSFFIENQLPLVVLPVEVEF
                     CLDQQAEFEQCIKDYLLSFKR"
     gene            complement(2170..2592)
                     /locus_tag="PLE23_09965"
     CDS             complement(2170..2592)
                     /locus_tag="PLE23_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HMX83631.1"
                     /translation="MKSIITLLIIFSCSFVIAQSPVGIWKTIDDKTGEAKSYIEIYES
                     NHKYFGKINKLLKSEPTKKCEKCPGARKNKPLVGMIIVENLSPYKDYWKNGTILDPES
                     GSEYGCSIWFENNNANELKVRGKHWTGLFRTQTWYKVK"
     gene            complement(2610..2948)
                     /locus_tag="PLE23_09970"
     CDS             complement(2610..2948)
                     /locus_tag="PLE23_09970"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HMX83632.1"
                     /translation="MNKNYSYFDLVYEITRNIPSGRVTTYGAIADYLGLGSARMVGWA
                     LNQVHGSYTDVPAHRVVNRLGELSGRNHFPDPDYMANMLKQEGIEVVDNKITEFKKYF
                     WHPENLDSIL"
     gene            2981..3592
                     /locus_tag="PLE23_09975"
     CDS             2981..3592
                     /locus_tag="PLE23_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83633.1"
                     /translation="MYKCKILADKISNLTDARYFSARFADIICFDPDAISADDPVKAI
                     VQMNLMKEWLAGPQIFVRTNFRSNEELERMIREVEPDGILISCFDQPDEALFHEKKVF
                     RELIVDQDNPEYIFEITGDYDGILIDVRQTGLLADSNFIQKLKQLTRQYIIFIKNDDL
                     NEAAVFTKQNVSFANLAINGMAEHRTGFRSYDDTDDILQQLEV"
     gene            complement(3598..>4846)
                     /locus_tag="PLE23_09980"
     CDS             complement(3598..>4846)
                     /locus_tag="PLE23_09980"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83634.1"
                     /translation="GIIDVVPFQKVALTGIASSYCFMDTTITINVTPPGGKLLVDGKP
                     QTTFNPAIAGPGVHSISYNYGSGKCADSKSIVVNVGDPVTVQLAFASDSICRGESSKI
                     SALGLGGSSFGNFNYFWNNGVGFGQTQFVFPEKTTTYTVTVDDGCSDPATADVLVYVH
                     PDFVVDKVYGPEVCFGDTTFTKLSGNPVADYTFNWLTNPVFIGDTYTGPPSTYLVQVV
                     NNNTGCSQEESIDLKGYPLIQANFTIPPTKDCFSSLDASINVLDHSVGGVSGYWDFGD
                     GSNINYNPQDGNLIHLYANEGDFTIKLHIENEGGCFSEDSLKLCVINEETIYAPNAFT
                     PDGDNLNDYFKFAGLGIKSIEWKIFNRWGEQLFVGNSMDDVWDGFKNNKRVPLGIYTY
                     VAVYKSEVSNTEKTLKGLVTVVY"
BASE COUNT         1436 a          958 c         1021 g         1431 t
ORIGIN      
        1 gtaacgaatt tggtagtagc aatatatatt gggtgttgtt tggcaatctt ttcaattaac
       61 caaggcagaa aattgggaat aaaatgtata accagacctg caagcataca aggtaggccc
      121 aatactgcaa gcagtaagtg aacaatgaaa gaaagtacag aggttggttc aatactttca
      181 ggaacctgct ctgcctttaa gtgccattta tgcatataat tttctaccac tgcaatgtgt
      241 gtacgtgccg actcaatgag taagggatta ttttgttcca gtcgattgat atcgtctgcc
      301 aatagctttc ctttaatgaa aaactgttcg gggtcggctt caataccagg attaaaccac
      361 cttagtaatc tttcaaaatc aggaatcaaa gcatcatttt cgagatggat attgagtttt
      421 ttgagttccc ggaatatttt ttccgttacg agcttcacgg ctgtcacttc atccttgcgg
      481 taagtctctt cgaattcttc gatgccgaat ggtgcagatg tactaatgaa ctgccccgac
      541 caaaaactgc ccgcatctat gtatgtgaca cccgatacta cgaaacgcaa tccaagctgg
      601 aaatcttttt tagactcggc actcaaagct atacgcgcag ctccggttct gagtggaagt
      661 atccgcctta cattgaggct cgtaccttct ggtgctacaa acaaagttcc tccctcgtgc
      721 agaaatatgt cgcaacgttc gaaactcctg accatgtcaa ttggttggtc ggtcgaaaaa
      781 tttgaccttt gaacaggaat gcaatagaat gtgttaaaaa accatcgtgt aaaccagtta
      841 ctgaacaact gcgactttgc aaggaagtga accattcgcc ttgaatgtag ggcaacattt
      901 attgggtcca tcatgccatt tgggtggttg cctatcagta acatcgggcc tttggtgttt
      961 aggtattgtc ggttgtggac aacagtattg ggataaaaaa gcctcaaccc aatgcctatc
     1021 aaggctttga agacaagata catgaactga agcaaacgca cccgaatcat cgtttgaaac
     1081 ttagcaagta atcttttatg cattgttcga attcggcttg ctgatcaaga caaaattcaa
     1141 cctcgaccgg taaaaccaca agaggtagtt gattttcaat gaaaaatgac ctgaaaagct
     1201 ctagacggcc tgcatctttt tcagttgtta agatgatttt attctcagac ttcatgctcc
     1261 taaacaggga atggatagct cgcatatctt cttccttgaa gtaatgatgg tcacggaatt
     1321 cgagcatttc aatactattg acattggcat gcaggaaatc aaccaagtag tcagagttgg
     1381 caatagcaga aatcagtaac acatctgtat cttctttcag attgtatgaa aatgaaggat
     1441 tgaaccaaaa ataaggacgt cggtatttga actttgaaaa gaagaccttt tggtgagaaa
     1501 aaggccggag agatttagta aatttttctg cgtcatcaag gcttagttta tccgggcatt
     1561 tggtgacaat gataacatct gcgcgtttgt acgactccgg aaattcgcga agcgaaccag
     1621 aaggcaataa aaaatcctta aaaaaaggcc tggaatgctg cgtgagtaga atattgagtc
     1681 cgggtattat ctggcgatgt tggaaggcat catccagcaa gattgtctgc agatcgggtt
     1741 ttgttatcag cattttcgga atggcaaaaa cgcggttttc gcacactgct acgaatacat
     1801 ctttgaattt tcttttaatc tgtaatggtt cgtcgccagc atcaagagaa gaatcgaatt
     1861 cgttgacgac cctatagccg gaggtttttc gtttgtatcc acgactgagt gtaccaatct
     1921 ggatatacgg actcagcaga cggatcagga attcgatatg tggagttttg cctgtgccac
     1981 ctactgacag attgcctaca gaaataaccg gaatatcaaa actaacgcct ctcagcagat
     2041 ctgctttata caaaagttcg cgaatgacaa tgaccgtacc gtatatcagt gaaaacggaa
     2101 aaaggataat tttaagaaaa atattgtttg tcataagagc aatatgcagg cagtcacaca
     2161 cttacccggt tatttcactt tgtaccaggt ctgtgtacgg aaaagtccgg tccaatgttt
     2221 gcctctcact ttcagttcgt tggcattatt gttttcgaac cagatgctac agccatattc
     2281 gctacccgat tctgggtcga gtatggtgcc gtttttccag taatctttat atgggctgag
     2341 gttttctaca attatcattc ccactaatgg tttatttttc ctggcacccg gacatttttc
     2401 acattttttg gtaggctccg atttgaggag tttgttgatt tttccaaaat atttatggtt
     2461 tgattcgtaa atctctatgt aagatttggc ttctccggtt ttatcgtcga tggttttcca
     2521 gatacctacc ggactttgag caataacaaa gctgcaggaa aaaatgataa gtaaagtaat
     2581 gattgatttc ataaatcgaa tagtatgggt taaagtattg aatcaagatt ttcagggtgc
     2641 cagaaatatt ttttgaattc cgttatttta ttatcaacaa cttctatacc ttcttgttta
     2701 agcatgtttg ccatgtaatc tgggtcaggg aaatgatttc ggccactgag ttcgccaagc
     2761 cggttgacta cacggtgagc cggtacatca gtatagctac catgaacctg gttaagcgcc
     2821 caacctacca ttcttgcaga accaaggccg agataatcag ctattgcacc ataagtagta
     2881 accctgcctg aaggaatatt gcgggtaatt tcataaacaa ggtcgaaata gctataattt
     2941 ttgttcaaaa gtcagatttt tgtgaaaatt aatgcatttt atgtataagt gtaagattct
     3001 tgccgataaa atttctaatc tgaccgatgc aaggtatttc agtgcaaggt ttgctgacat
     3061 tatttgcttt gacccggatg ctatatcggc agatgacccg gtaaaagcta ttgtgcaaat
     3121 gaacctgatg aaagaatggc ttgcaggtcc acaaatattt gttcgtacca atttcaggag
     3181 caatgaagaa ttggaaagaa tgatacgcga ggtagaaccg gatggtattt tgatatcatg
     3241 ttttgatcag cctgacgaag ctttgtttca tgaaaaaaaa gtgttcagag aattaatagt
     3301 tgaccaagac aacccggaat acatttttga aataaccgga gattacgatg gcatattgat
     3361 tgatgtgcgc caaaccggac ttctggcaga ttcaaatttt atacaaaaac taaaacaact
     3421 gacgaggcag tacattattt ttattaaaaa tgatgactta aatgaagcag cagtatttac
     3481 caagcaaaat gtgtcttttg caaatttggc gataaacggt atggcagaac accggactgg
     3541 attcaggtct tatgatgata ccgacgacat cttacaacaa cttgaagtct gaaaatctta
     3601 atagacaacg gttacgaggc cttttagtgt tttttcagta ttgcttactt cgcttttgta
     3661 aactgcaacg taagtatata tacccaacgg tacgcgtttg ttgttcttga atccgtccca
     3721 gacatcgtcc atgctgttac ccacgaatag ttgttcgccc cacctgttaa atatcttcca
     3781 ctctatagat tttataccaa gaccggcaaa cttgaagtaa tcattgaggt tgtcaccatc
     3841 gggggtgaat gcattggggg cataaatagt ttcttcgtta atgacacaga gtttcagaga
     3901 atcttcagag aaacacccac cttcattttc gatatggagt tttattgtaa agtcaccttc
     3961 attcgcgtaa agatgaataa ggttgccatc ttgtgggtta taattgatat tacttccatc
     4021 gccaaaatcc cagtatcctg atactccacc tacactgtgg tcaaggacat tgatgcttgc
     4081 atcgagtgat gaaaaacagt cttttgttgg tgggatggta aaattggcct gaatcaaagg
     4141 ataacctttc aagtcaatac tttcttcctg gctacagccg gtattattat tgaccacctg
     4201 tacaagatat gttgaaggag ggcccgtgta tgtatcacct atgaatacag gatttgtaag
     4261 ccagttaaaa gtgtagtcgg ctaccggatt cccggacaat ttggtaaatg ttgtatcgcc
     4321 aaagcacact tcagggccat acaccttatc aacgacaaaa tccggatgta catataccaa
     4381 cacatcagct gttgcgggat ctgaacagcc atcatcaacg gtgacagtat aggtggttgt
     4441 tttttcggga aagacgaact gagtttggcc gaatcctaca ccattattcc aaaagtaatt
     4501 aaaattccca aatgagctgc ctccgagtcc tagcgccgat attttcgaac tctccccgcg
     4561 acagatagaa tctgaggcaa aagccagctg tacggtaaca ggatcaccta catttacaac
     4621 gattgatttt gagtctgcac attttccact accataattg tagcttatac tgtggacgcc
     4681 agggcctgca atggccggat taaacgtggt ctgcggctta ccatcaacta ataatttgcc
     4741 acctggaggg gttacattga tggtaatagt ggtgtccata aaacaatacg acgaggcaat
     4801 acctgtaagt gccacttttt ggaatggtac tacatcgatg atgccg
//
LOCUS       DAOQQY010000222         4815 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8142_length_4815_cov_5.955882, whole genome shotgun sequence.
ACCESSION   DAOQQY010000222 DAOQQY010000000
VERSION     DAOQQY010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4815
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8142_length_4815_cov_5.955882"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /locus_tag="PLE23_09985"
     CDS             <1..1889
                     /locus_tag="PLE23_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMX83635.1"
                     /translation="YDDFIGEVLSSLKVADTAVMVLNAKSGVEVGTEIIWEYIEKFKT
                     PAMLVINQLDHEKADYDNCVQQAIDRFGSHLLPVQYPLNQGTGFNSIVDALRMVMYVF
                     PTGGGKPEKKPIPESEMPRALEMHNKLVEAAAENEDGLMDKFFEQGTLDEADLTKGLT
                     IAMANQQIFPIFCASGAGNMGTGRIMGFINDICPSPIDRPDAILEDGNTLKCDSNDDT
                     TIFIYKTLTEPNVGMVSYFKVYSGTLKTGDELTNHNNRTVERFNQIFVSNGKIREAVP
                     ELKAGDLGVTVKLKNSHSNNTLTAKNSDKGIEPIHFPEPRIRAGVKPPSKQDMEKLVK
                     ALHQIQEEDPTLILEQNSQLKQTIMHGQGQLHMDIIKYRIEKVNGISMEFEKPKIPYR
                     ETITREANETYRHKKQSGGAGQFAEVHIRIEPYHEGMPDPAGLTVKKTEVDELPWGGK
                     LVFVWCIVGGSIDAKFSNAIKKGVMQKMENGPLTGSACTDIRVSVYDGKMHPVDSNDM
                     AFQIAGAQAFKNAFKNSHPQILEPIQNLEILCPDEVMGDIMGDLQTRRAMIVGMDSDG
                     HYQKILAKVPLAELYQYNSTLRSVSQGRAKFKMSFAEFQSVPTDIQQKLAAEHTDHDH
                     DEDI"
     gene            2002..2451
                     /locus_tag="PLE23_09990"
     CDS             2002..2451
                     /locus_tag="PLE23_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83636.1"
                     /translation="MKKILFVVALLGFFTYGASAQSCCAKKSSASKASCSSSAAATTA
                     SNDEAAIAKLVSNDPSIEKRTDDATGSTYYVKKEVCEKSGKVSYNKVEYCSKEAKFVN
                     VSPSDASGTAKASCTSKASCSKESKSGSCCAKKSASADAKPASNDMK"
     gene            2621..3640
                     /gene="galE"
                     /locus_tag="PLE23_09995"
     CDS             2621..3640
                     /gene="galE"
                     /locus_tag="PLE23_09995"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMX83637.1"
                     /translation="MAKILVTGGCGYIGSHTIVDLIENGFDPISLDNLTNSDESALEA
                     IRQITGKTIPNYKVNLIDRNALNTVFEENPDIEGIIHFAALKAVNESVIHPLLYYSNN
                     LKGLINLLEAALAYKVHHFIFSSSCSVYGDSDILPVTETTPLQKAQSPYAYTKQAGER
                     IITDLATGHTHFKTILLRYFNPAGAHPTALMGESSKNIASNLVPVITETAFGKRKNIT
                     VFGTDYDTRDGSCIRDYIHVMDIAHAHTLAMKHILSHTQATTDTEIFNLGSGNGTTVI
                     EAIKAFEKISGISLNVIFGPRREGDVVAVYADNQKARTVLGWIPKYDIDQIMQTAWNW
                     EQKRS"
     gene            complement(3963..>4815)
                     /locus_tag="PLE23_10000"
     CDS             complement(3963..>4815)
                     /locus_tag="PLE23_10000"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMX83638.1"
                     /translation="QYYVWLPILKVREEPNQDAKEVAKLKKGDQVEYLGIKTQKTETI
                     TISKKVFEDNWMKIKTDDGVQGWVFGAGINIKDVKEDLSPTPYDDCFTLIAAREINDY
                     FKCMSQVRKQVQKEQTNRCYFTANSITLSMIDGKPLKLINEVGDTKKSYNFMLYYPGI
                     EQFVVQTQTGVLEEYLLIDAHNGNKITTWGLPKVSPGKDFIVSASSNYIFQGAPNGIQ
                     ILQRDGKVIKKVWEREIESYEPYAPKWLDNNTIGVTLKPRDGAKKFGSKFALIRKESP
                     TEWKMEY"
BASE COUNT         1533 a         1031 c          922 g         1329 t
ORIGIN      
        1 ggtacgacga ctttatagga gaagtgttgt cttcactcaa agtagctgac actgcagtaa
       61 tggttctaaa tgccaaaagc ggtgtagaag taggcacaga aataatctgg gaatacattg
      121 aaaaatttaa aacgcccgcc atgttggtta ttaaccagct ggaccatgaa aaggcagact
      181 atgacaattg tgtccaacaa gctattgacc gtttcggaag tcaccttctg ccggtacaat
      241 atccactcaa tcaaggcacc ggtttcaaca gtatagtgga tgccctccgt atggtgatgt
      301 acgtgttccc gacaggtggt ggcaaacctg aaaaaaaacc aatacctgaa agcgaaatgc
      361 cacgcgcgct ggaaatgcac aacaaactcg tagaggcagc agctgaaaac gaagacggac
      421 taatggacaa atttttcgaa caaggcacac tggatgaagc agacctgacc aaaggcctga
      481 ccatcgccat ggccaaccag cagatattcc ctatattctg cgcatcgggt gcaggcaata
      541 tgggcaccgg acgcatcatg ggtttcatta atgacatttg tccctcaccc attgaccgac
      601 cggatgccat actggaagat ggcaatacac ttaaatgcga ctccaatgac gatactacca
      661 tatttatata caaaacactt accgaaccca acgtgggaat ggtctcttat tttaaggtat
      721 attctggaac actaaaaacc ggtgacgaac tcaccaacca caacaaccgc actgttgaac
      781 gatttaacca gatattcgta tctaacggaa aaattcgcga agcagtacct gagctaaaag
      841 caggtgatct gggagtgacc gtaaaattaa agaattcgca ctccaataat acactcactg
      901 ccaaaaactc agacaaaggt atcgaaccaa tacattttcc tgaaccaaga atccgtgcag
      961 gtgtcaaacc acccagcaag caagatatgg aaaaactcgt gaaggcactc caccagatac
     1021 aggaagaaga cccaacactc atacttgaac aaaacagtca gcttaagcaa accatcatgc
     1081 acggtcaggg acagctccac atggatatca tcaagtatcg tatagaaaag gtaaatggta
     1141 tatcaatgga atttgaaaaa ccaaaaattc cgtaccgcga aaccatcaca cgtgaagcaa
     1201 acgagaccta ccgccataaa aaacaatcgg gtggtgccgg acaatttgcc gaagtacaca
     1261 tccgcataga accataccat gagggcatgc ctgaccctgc aggcctgaca gttaaaaaaa
     1321 cggaagtgga cgaattgcca tggggaggca aactagtatt tgtttggtgt atcgttggcg
     1381 gctctattga tgcaaaattc tcaaatgcca ttaaaaaagg tgtcatgcaa aaaatggaga
     1441 acggcccgct aacaggttct gcatgtacag atatccgtgt atctgtatat gatggcaaaa
     1501 tgcacccggt tgactccaat gatatggcct tccagatagc aggagcacaa gccttcaaaa
     1561 atgcattcaa aaactcgcac cctcagatac ttgaaccaat acagaatctc gaaatactgt
     1621 gcccggacga agtaatgggc gatataatgg gcgacctaca gacacggcga gcaatgatag
     1681 taggtatgga tagcgatggt cattaccaga aaatactggc aaaggttcca cttgctgagc
     1741 tttatcaata taactctaca ctgcgttcag tatcacaagg tagggctaag ttcaaaatgt
     1801 catttgccga attccagtct gtaccaactg atattcagca aaaactggca gccgaacata
     1861 ccgaccacga ccatgatgaa gacatttaat gaaaataagt taaaattgtc ttaatccctt
     1921 tgctgaactt gatttaatat gcgtgaaagc ataactttgt agcgatttat gtattaatta
     1981 attaatttaa taattttaga aatgaaaaaa attctttttg ttgttgccct actaggcttt
     2041 ttcacgtacg gtgcaagtgc acagtcatgt tgtgcaaaaa aatcttctgc ttctaaagcg
     2101 tcatgctcat cttcagctgc tgctactact gcaagcaacg atgaagcagc tatcgcaaaa
     2161 ttggtgtcaa acgacccttc aattgaaaaa agaaccgatg atgctacagg ttctacttat
     2221 tatgtaaaaa aagaagtttg cgaaaaaagc ggcaaagttt cttataacaa agtagaatat
     2281 tgctcaaagg aagcaaaatt cgtaaacgtt agcccttctg atgcttcagg tactgctaaa
     2341 gcttcttgca ctagcaaagc ttcttgctct aaagaaagca aatcaggatc ttgctgcgct
     2401 aaaaaaagtg cttctgctga tgctaagcct gctagcaacg acatgaaata attcattttt
     2461 tcaagatata agaaaggtga cttactacgg tttgtcacct tttttatttt ttgatcataa
     2521 tggttttgat cactcgtacc atcgacatgc aactgaagtt cgaaaatcgg gctacccaaa
     2581 tactttaaca gcacacaatt gattaatttt gcggaaaaac atggcgaaaa tattggtaac
     2641 aggtgggtgt ggttatatcg gtagtcacac tattgtagat ttaatcgaaa atggcttcga
     2701 cccaatttcc ttagataatc tgacgaactc tgatgaatcg gcactagagg ccatcaggca
     2761 aattaccggc aaaacaatac ccaattataa agtcaatcta atcgaccgaa acgccctcaa
     2821 tacagtattt gaagaaaatc ccgacatcga aggtatcatt catttcgcag ccttaaaggc
     2881 cgttaatgaa tcggttatcc atccactgct gtattattcc aataatctta aaggtctgat
     2941 taatctgctt gaagctgctc tggcatataa ggtgcatcac ttcatttttt catcatcttg
     3001 ctctgtatat ggtgactctg atatcttacc tgtaaccgag acaactcctc tgcagaaagc
     3061 ccagtctccg tatgcctata ccaaacaggc aggcgaacgc atcattactg acttggcaac
     3121 agggcacacg catttcaaaa ccatattatt gaggtatttt aacccggcag gtgctcaccc
     3181 tactgccctt atgggagagt cttcaaaaaa tatagcaagc aaccttgtac ctgtcattac
     3241 tgaaacagca tttggaaaaa gaaagaatat aactgtattt ggtacagact atgacactcg
     3301 cgacggttcc tgtattagag attatataca tgtaatggac atagcacatg cgcatacact
     3361 tgccatgaag catattctat cacatacaca agctaccacc gatacagaaa tattcaatct
     3421 cgggtcgggt aatggcacga ccgtcattga agcaataaag gcttttgaaa aaataagtgg
     3481 catatcactc aatgttatct ttggaccacg cagagagggt gatgtagtag cagtatatgc
     3541 cgacaatcaa aaagcacgca ctgtccttgg gtggataccc aaatacgata ttgaccaaat
     3601 catgcaaaca gcctggaatt gggagcagaa aaggtcctaa gtattttacc cgactatttt
     3661 ttcaacacat tttagttaaa tttttactaa atggcatccc tgctgtatca aaatgtgcct
     3721 ttcacacgtc attggtataa gtgtactgtt ttttcatact atttcctgaa gactgttatt
     3781 agtatagcag tcttcatttt ttttaaccaa taacacgtat taggaattta tacgaaaatg
     3841 attagtacaa aaaatccata ttaaagagat ataagtatag tgaactttgg tttttaagct
     3901 tttgaaatgg atttgaagta agaattatat gccgtaatgg gcgcctaatg tgttatctgg
     3961 gcttaatact ccatcttcca ttctgtagga gactcttttc ggatgagtgc gaattttgac
     4021 ccgaactttt tagctccatc tctcggctta agtgttacac ctatggtatt gttgtcgagc
     4081 catttaggtg cataaggttc gtaactttca atttctcgtt cccaaacctt ctttattact
     4141 ttaccatccc tttgcagaat ttgaatgcca tttggtgcac cttgaaagat atagttactg
     4201 cttgcagata caataaaatc cttgcccgga gaaactttag gcaggcccca ggtagttatt
     4261 ttgttgccat tgtgtgcatc tatcagcaaa tattcttcaa gtacaccagt ctgcgtttga
     4321 acaacgaatt gctctatacc cggataatac aacatgaaat tataactctt ctttgtatca
     4381 cctacttcat ttatcaactt tagcggtttt ccatcaatca tggataaggt tatactattg
     4441 gcagtaaaat agcaccgatt tgtctgctct ttctgtactt gcttacgaac ttgcgacata
     4501 cacttgaagt aatcattgat ctcgcgtgca gctataagtg taaaacaatc atcataaggt
     4561 gtgggtgaca aatcttcttt tacatctttt atgttgatac cggcaccaaa aacccaaccc
     4621 tgtacgccat catctgtttt aatcttcatc cagttatctt caaaaacttt cttgctgatg
     4681 gttattgttt ctgttttttg agtcttgata ccgaggtatt cgacctgatc accctttttt
     4741 aactttgcaa cctcctttgc atcctgattg ggttcttcac gaactttcag gataggcaac
     4801 catacataat actgt
//
LOCUS       DAOQQY010000223         4811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8154_length_4811_cov_5.309083, whole genome shotgun sequence.
ACCESSION   DAOQQY010000223 DAOQQY010000000
VERSION     DAOQQY010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8154_length_4811_cov_5.309083"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..987)
                     /locus_tag="PLE23_10005"
     CDS             complement(169..987)
                     /locus_tag="PLE23_10005"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HMX83639.1"
                     /translation="MKVAILDMYNDKPGQHGMRCLHDILREYAPEAEVSVFNVRAKVE
                     IPDLSYDLYISSGGPGDPTEYDERWSDKYFNFIDQLEQWNKQHIDSPKYLFAICHSFQ
                     LLCAHKRIGAITARKKFSFGVTAIRKTDDGKTDDLLKHFDEVFYAADHRYFQVVDPDF
                     SKIEEADIHILAKEECNDDNSEDSLMVVRFSPHIIGTQFHPEVDVLGMKDTITKENIR
                     LEVEKVFGKGRLVRIQKDINDPQKIDKMYHTLLPQFIHLCKETKAGIPVNEPMI"
     gene            1148..1753
                     /locus_tag="PLE23_10010"
     CDS             1148..1753
                     /locus_tag="PLE23_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HMX83640.1"
                     /translation="MANFERRGAQAKKGSLRGMIVILLIIVLLGGMLLPSIKGFFGDN
                     ASAPASDNGVNNTSESIPSTSTNEQGGEPKFVKQGELKLLDANKKELKKIDIELATTE
                     AKRAQGLMYRSYMGPDQGMLFIFDAQQPQAFWMENTKIPLDIIYIDKNMKIVSIAKNT
                     VPFSRQSIPSKSPAQYVLELNAGYADAYKIKEGDYISYTTL"
     gene            1799..3820
                     /locus_tag="PLE23_10015"
     CDS             1799..3820
                     /locus_tag="PLE23_10015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMX83641.1"
                     /translation="MKKIKYLLLLLTLYLRSTNIPAQQVLLEPEMLWKLGRVSMDDIS
                     ADKKTLLYGITDMDIAANKGNRNLFIQDVNAATPKQLTTLDGSEWNARFTPDGKRVAF
                     ISKGNLWEVELDGTNAHKVSDLELSGFEFSPDGTKILYTADYKFGKTTTEKYPDLPYA
                     NARIYDDLMYRHWDHWEDEIASNVHYISYKDGQLNGEGKNIQKEPYDSPLAPHGSIDE
                     ISWSADSKYIAYTCKKKSGTEYALSTNSDIYLYELATGKTTNISEGIMGYDKSPKFSP
                     DGKYVVWLSMERDGFEADKPRLMLAEVKNPKKTRTDITKSIDREADGPQWAPDSKKIY
                     FLLNDQGTVQIAYYDLAISKIERLTSRLQNYNSILVGNGVIFAEKTTMTEPQEIVKIE
                     ADGTEKGFSNFNTTFWNTIKKPEVKKRMVKTKDGKEMLAWVIYPPDFDPSKKYPTLLY
                     CQGGPQSTVNQFFSYRWNFDLMASKGYIVIAPNRRGLPSFGREWNDEISGDYGGKAMQ
                     DLLSAIDDISKEPYVDKNRRGAVGASFGGYSIFWLAGNHEKRFKTFIAHAGMFNITSW
                     YGTTEEMFFANWDQKGPYWKKPVPKGYTKFTPHHFVDNWDTPILVIHGEKDFRVPVSE
                     GMQAFNAARLKGIPARFLVFPEENHWIVQPQNSLLWQREFFGWLDKYLK"
     gene            3851..4297
                     /locus_tag="PLE23_10020"
     CDS             3851..4297
                     /locus_tag="PLE23_10020"
                     /inference="COORDINATES: protein motif:HMM:NF019241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HMX83642.1"
                     /translation="MKNDFSSFFYQYLDQISKEINSYDHEEDIWKNVGETKNSAGNLT
                     LHILGNLNHFVGAALASNGYVRDREAEFADKDVPRAKLLEEIEHVKALIVKTLPHLNN
                     LSDSYPEGYWNDSGSIHFQLLRLLSHLAYHTGQINYHRRGFASKDV"
     gene            complement(4377..>4811)
                     /locus_tag="PLE23_10025"
     CDS             complement(4377..>4811)
                     /locus_tag="PLE23_10025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HMX83643.1"
                     /translation="GDWWTAEDAKAYESRAQKVVDQYNAYTVYDTVHVKGELTLGENI
                     ADLGGLAIAFDAFKKTAEFKEGKSIDGYTPEQRFFLGFGQIWRMKNTQKMMLNRINTD
                     PHSPEEFRIKGSVSNFTPWYEAWKITPQDKLYKPENERIKVW"
BASE COUNT         1479 a          993 c          973 g         1366 t
ORIGIN      
        1 ctatcattaa ccgttccttg aggtagccag gcataaatac cggtgtttca gcaattctaa
       61 aaccagagtc gcaaccgaaa tattccctta tgtctgaaag taagtttgaa tacttatcgt
      121 cagaaaattt tttattgaaa tcggctcgta actcaggtat catatctgtc agatcattgg
      181 ttcgttaaca ggaatgccgg ctttcgtttc tttgcaaaga tggatgaatt gtggtaagag
      241 ggtatggtac attttgtcaa tcttttgtgg atcatttata tccttctgta ttctcaccaa
      301 tcttccttta ccgaatactt tttctacctc gagtctgata ttttctttgg taatcgtgtc
      361 tttcataccg agaacatcta cttccgggtg aaattgtgta ccgatgatat gtggactgaa
      421 tctgaccacc atcaatgaat cttcgctgtt atcgtcgtta cattcttctt tggcgagaat
      481 atgtatgtca gcttcttcta tttttgaaaa atcaggatct acaacctgaa aataccggtg
      541 atcggcagca taaaacactt catcaaaatg tttgagtaag tcatctgttt tgccatcatc
      601 cgtcttccgg atagctgtga cgccaaagga aaatttcttc cttgccgtta tggcaccaat
      661 tcgcttatga gcgcacaaca attgaaagga atgacaaatc gcaaacaagt attttggact
      721 atcaatatgt tgcttgttcc attgctctaa ctgatctatg aaattgaaat atttatcgga
      781 ccatctttca tcatactcag tcggatcgcc ggggccaccg gatgaaatgt acaaatcgta
      841 tgataaatca ggaatttcaa cctttgccct gacattaaaa acagatactt ctgcctcagg
      901 tgcatactcg cgtaatatat catgcagaca cctcatacca tgctggcccg gtttgtcgtt
      961 atacatgtcc aaaatggcta ctttcactga taattctctt tgtttgttga atataaagcc
     1021 gcaaagttac ggatttctat taaaaaaaca cagatgtgga taagcattta ttgtacactt
     1081 caggtcgatt ttaaagaatt ataaatttat ttatttaatt ttgcgttcat aaaaactcat
     1141 ttatattatg gctaattttg aaagacgtgg tgctcaggca aaaaagggct cattaagagg
     1201 catgattgtc attttactga taatcgtttt actcggtgga atgctccttc cttccatcaa
     1261 aggctttttt ggagacaatg catccgcacc ggcatcagat aatggcgtaa acaatacttc
     1321 cgagtctata ccttcaacta gtacaaacga acaaggcgga gaaccaaagt ttgtcaaaca
     1381 aggtgaactc aaattgctcg acgctaataa aaaggaactc aagaagatcg atatagagtt
     1441 ggcaacaaca gaggcaaagc gagcccaggg cttaatgtat cgttcttata tgggcccgga
     1501 ccaagggatg ctttttatct tcgatgcgca acagccacaa gccttttgga tggaaaatac
     1561 aaaaattcca ctcgacatta tttacatcga taaaaatatg aaaattgtat ctattgctaa
     1621 gaatacggtt ccgttttcgc ggcaatcaat tccgtctaag tcacctgcgc aatatgtcct
     1681 ggagctgaat gccggatatg ctgatgctta caagattaag gaaggtgatt atatctccta
     1741 tactacactg taagttttac ttttgcaaag gcgcatttca actattaaac catttaaaat
     1801 gaaaaaaatc aagtatctgc ttctcttgtt aacgctgtat ctacgcagta ccaacatacc
     1861 agcccagcaa gttctgctcg aacccgaaat gctgtggaag ctaggtcggg taagtatgga
     1921 cgacatttct gccgacaaaa agaccttgct ttatggcatt acagatatgg acatcgcagc
     1981 caacaaagga aaccgaaacc tgttcattca agatgtaaat gcggctactc caaaacagct
     2041 aaccacgctt gacggcagcg aatggaatgc ccgatttacg cctgacggca aaagagtagc
     2101 atttatttcc aaaggcaatc tttgggaggt agagctagat ggcaccaatg cacacaaagt
     2161 ttcagatctt gaattgagtg gattcgaatt ttcacctgat ggcaccaaaa tactttacac
     2221 agccgactac aaattcggca aaactactac agagaaatat cctgacctgc cttatgccaa
     2281 tgcccgtata tacgacgacc ttatgtaccg ccattgggac cactgggaag atgaaatagc
     2341 ctcaaacgtc cattatattt catacaagga tggtcagctg aacggagaag gcaaaaacat
     2401 tcagaaagaa ccttatgaca gcccattggc tccgcatggt tctattgatg aaattagttg
     2461 gtcggctgac agcaaataca ttgcctatac ttgtaagaaa aaatcaggaa ccgaatatgc
     2521 attgagtacc aattcagata tataccttta cgagcttgcc accggaaaga ccaccaacat
     2581 ttctgagggc atcatgggtt atgacaagag tccaaagttt tctccagatg gcaaatatgt
     2641 agtgtggctc agtatggaac gagacggatt tgaggctgac aaaccgaggc tgatgctcgc
     2701 cgaggttaaa aacccaaaga aaacacgaac ggatattaca aaatccattg accgtgaagc
     2761 agacgggcca cagtgggcgc ctgacagcaa aaaaatatat ttcctgttaa atgaccaggg
     2821 aactgtgcag attgcttact atgatcttgc tatcagtaaa atagaaagat taaccagcag
     2881 attacaaaat tataacagta ttctggtagg aaacggagta atctttgctg aaaagacaac
     2941 gatgaccgag cctcaggaaa tagtaaaaat agaggccgat ggtactgaaa aaggcttttc
     3001 taacttcaac accacattct ggaataccat taaaaagccg gaagtaaaaa aacgcatggt
     3061 aaaaaccaag gacggcaaag aaatgctagc atgggttatt tacccaccgg attttgaccc
     3121 aagtaagaag taccctactc tgctttattg tcagggtggg cctcaaagta cagtcaacca
     3181 gtttttttct taccgatgga atttcgactt aatggcaagc aagggttata tagtgatagc
     3241 acctaacaga cgagggcttc catcgtttgg gcgtgaatgg aacgatgaga ttagtggaga
     3301 ttatggtggc aaggcaatgc aggatttgct ttcggccata gatgatattt caaaggaacc
     3361 atacgttgac aaaaacagac gtggagcggt aggtgcaagt tttggaggtt acagcatttt
     3421 ctggctggct ggcaatcatg aaaaaaggtt caagacgttc attgcacatg ccggtatgtt
     3481 caacatcacg agctggtatg gtacaacaga agaaatgttc tttgccaact gggaccaaaa
     3541 aggaccttat tggaaaaaac ctgtaccaaa gggatatacc aaattcaccc cgcatcattt
     3601 cgtagataac tgggatacgc ccatactcgt cattcacggt gaaaaagact tcagggtacc
     3661 cgtttcggaa ggtatgcagg catttaatgc agcaaggctg aaaggcatcc cggccagatt
     3721 ccttgttttt cctgaagaaa accactggat tgtacagcca cagaacagct tgctttggca
     3781 gagagaattc tttggttggc ttgataaata tcttaaataa actacgtcat ttcaactaat
     3841 cattactaac atgaaaaatg acttttccag ctttttttat cagtatcttg accagatatc
     3901 caaggaaatt aactcctacg atcatgaaga ggatatctgg aaaaacgttg gtgaaaccaa
     3961 aaattcggcc ggcaacctga cactgcatat actaggcaat ctcaaccact ttgttggtgc
     4021 tgcgcttgct tccaatggct atgtacgtga cagagaagcc gagtttgctg acaaggatgt
     4081 accaagggca aaattgctcg aagagataga gcacgtaaaa gcgcttattg taaaaacgtt
     4141 accccacctc aataacctgt ctgattcgta cccggaaggc tattggaacg actccggttc
     4201 tattcacttt cagcttcttc gtttattgag ccacctcgcc taccataccg gtcagattaa
     4261 ttatcacaga agaggattcg cgagtaaaga tgtataattt ttttagcaga aagctaatga
     4321 taaaaaaagg ctcctgaatc tattttcaga agcctttttc atttacattt cgaatattac
     4381 caaaccttta tcctttcatt ttcaggttta tacagtttat cttgtggtgt aattttccag
     4441 gcttcatacc aaggtgtaaa gttacttaca gatcctttga ttctgaattc ttccggagag
     4501 tgagggtcag tattgatgcg gtttagcatc attttctggg tatttttcat tctccatatc
     4561 tggccaaaac caaggaagaa tctttgttcg ggagtgtaac cgtcgattga ttttccttcc
     4621 ttaaattcgg ctgttttctt gaatgcatca aatgcaatgg caagtccacc caggtcggcg
     4681 atattttcac ccaaagtaag ctcacccttt acatgtactg tatcatatac ggtatatgca
     4741 ttgtattggt caactacttt ttgagcacgt gattcataag cttttgcgtc ttcggcagtc
     4801 caccagtcgc c
//
LOCUS       DAOQQY010000224         4810 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8159_length_4810_cov_5.761725, whole genome shotgun sequence.
ACCESSION   DAOQQY010000224 DAOQQY010000000
VERSION     DAOQQY010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8159_length_4810_cov_5.761725"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /locus_tag="PLE23_10030"
     CDS             <1..1035
                     /locus_tag="PLE23_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMX83644.1"
                     /translation="VEKNGSVFLAGPYLVKAAIGEDIDQETLGGATTHSEISGVTDYK
                     MPDDKTCLDTIRDLVDKIGAPDKAGFDRIKPAIPATDPKRIYGLYPATAKPYDMTELL
                     KCLVDDSKLTYYKEEYGKTIICAYARIDGWAVGIVANDRKIVKSGKGEMQIGGVIYSE
                     SADKAARFIMNCNQKKIPLVFLHDVTGFMVGSKSEHGGIIKDGAKMVNAVANSTVPKF
                     SIVVGNSYGAGNYAMCGKAYDPRLIVAWPTAKIAVMGGAQAAKTILQIQLASLKASGK
                     EADEEAAQQLYDKIKQRYDDQTTATYAASRLWVDAIIDPLETRKIISMGIEMANHAPV
                     ERFNPGVIQT"
     gene            1135..1623
                     /locus_tag="PLE23_10035"
     CDS             1135..1623
                     /locus_tag="PLE23_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83645.1"
                     /translation="MKKLFLLLIISASVQFGFAQKFELKINPVIMPFNVWQINGEFLV
                     NEDIGIEPGVIFIPEEDISLFSVLGKYYFSPKKGNDGFHIGMFGQFIVPDAYFGLGFY
                     TGYKWLSRRGVTFELGAGIGRILNPERSYGYYSETNITGTGQATVGYRFGYGKTQVEK
                     KM"
     gene            1635..1958
                     /locus_tag="PLE23_10040"
     CDS             1635..1958
                     /locus_tag="PLE23_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83646.1"
                     /translation="MNRIESQSFFDGQIQLLTGLNEFRESLWVNGFALRNQLTDEEIL
                     PFISFFDIIGITQVGDVLNITFKIYPDGLKSYTIEVNPFLKTFRYKGKEYPVSCFEEM
                     FVEKG"
     gene            complement(2075..2797)
                     /gene="bshB1"
                     /locus_tag="PLE23_10045"
     CDS             complement(2075..2797)
                     /gene="bshB1"
                     /locus_tag="PLE23_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HMX83647.1"
                     /translation="MKLDILAIGVHPDDVELSCVGTLLHYIDQGKKVGLLDLTKGELG
                     TRGNAQLRTQEAMESARLMGATVREQLDLADGFFEINKESILPIIEVIRRYRPDIVFA
                     NSNSDRHPDHGRSARLEDDACFYSGLRKIETIDKLTGQPQEAWRPKLVLHYIQDYHLV
                     PDFVFDITPYFDRKIELIKTFRSQFYDPDSAEPDSPISGKDFLEFNAARSRDFGRPAN
                     MTFAEGFQVNRLFGVKDIFDLV"
     gene            complement(2813..>4810)
                     /locus_tag="PLE23_10050"
     CDS             complement(2813..>4810)
                     /locus_tag="PLE23_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83648.1"
                     /translation="QGQPMWLRKVLAALRFIVLFLLAALLLAPVLKSKKTDIKKPVIV
                     IAQDVSESVTADLKDGDQAKYLEQVKQLASSFGKDYDVKTYSFGEKSREGLAEKFSDK
                     STDISEVLTTVTDIYNNQNLGAIILATDGINNQGSDPGYTAGLINVPVYPIALGDTIP
                     KKDLYIKKVYHHDIAYLKDKFTIQADIAARNCQGKSTHWQLSRIDGKTSQILQKGNLT
                     IDNKDFFKSVDMVLDADKPGVQHYRISLSKVDGEMTTVNNVKDFYVDIIDSRLKILIL
                     ANGPHPDISAIRQVISTNQNYVVETSLLADFKKKVTDYDFVILHQLPSRNSDVSNILQ
                     QLDAAKKPRLFVVGTQTDIYKFNKIQSLLTINGDGRNTNDAQASLVQNFGLFTLDPSV
                     SQAIEQFPPLTTMFGDYKLTGNASVLLMQKIGKIDTNYPLLMFGEFNGIKTGILAGEG
                     IWKWRLFDYLQRQNHNIIDQLIDKSIQYLSVKEDKRKFRVTMPKNIFLENEQISFDAE
                     VYNNSYELINDGDVSMTIKNENGKEFPYTFSKTGKYFNLNAGYFPPGAYSFVAKTSGI
                     GDKLIFEGKFEVQPVQLELYETTANHGLLKLLAQSTSGQMVYKDEMDKLHNLVVSSDR
                     LKPVLYQSNITQPVINLKWLFFLLLALLSAEWFMRRYYGSY"
BASE COUNT         1394 a         1033 c         1026 g         1357 t
ORIGIN      
        1 gtcgaaaaaa acggatcggt attcctggca ggcccatacc tcgtaaaagc agctatcggg
       61 gaagatattg atcaggaaac actcggtggc gccactactc acagcgaaat atcaggcgta
      121 accgattata aaatgccgga tgataaaact tgtctggaca ccatccgaga ccttgtggac
      181 aaaatcggag caccggataa ggctggtttt gacagaataa aacctgctat acccgctact
      241 gacccgaaac gtatctacgg tctctaccct gctactgcca aaccgtatga catgacagag
      301 ttgctcaaat gtctggtaga tgacagcaaa ctcacctatt acaaggaaga atacggcaaa
      361 accatcattt gcgcgtatgc aagaatagac ggctgggcag taggaatcgt cgccaacgac
      421 cgcaaaatcg taaaatccgg caaaggcgaa atgcagatag gcggtgtcat ctatagtgag
      481 tcagccgaca aggctgcacg attcatcatg aactgcaatc aaaagaagat accactggtt
      541 tttctgcatg atgtcaccgg attcatggtt ggctcaaaat cagaacatgg tggaatcata
      601 aaggatggag ccaaaatggt gaatgctgta gccaactcta ctgtaccgaa gttcagcatt
      661 gtagtaggca actcctatgg cgcgggcaac tacgccatgt gtggtaaagc ctacgaccca
      721 cgcctcattg tagcatggcc taccgcaaaa atagcggtaa tgggtggtgc acaagcagct
      781 aagactatcc tgcaaataca actcgcttca ctcaaagcca gtgggaaaga agcagatgaa
      841 gaagcagcac aacaattgta cgacaagatt aagcaaagat acgatgacca aacaacagcc
      901 acgtatgctg catccagact gtgggtagat gccatcatcg acccactcga gaccagaaaa
      961 atcatatcta tgggtataga aatggccaac cacgctccgg tcgaacggtt caatccggga
     1021 gttatacaga catagtatcg tattaataaa aaacggggtc atagcttact ttaaaggcaa
     1081 cgatttgcta aggatattcg ttttaatctt aacaaatatt aaacctatta aagaatgaaa
     1141 aaactattct tgctcctcat catttctgct tcagttcagt ttggctttgc ccaaaaattc
     1201 gaacttaaga taaaccctgt tattatgcct ttcaacgttt ggcagatcaa cggagaattt
     1261 ttggtaaatg aagatatagg tattgagccg ggtgtcattt tcatacctga agaggatata
     1321 agtttatttt cggttttggg taaatattat ttttcaccta aaaagggcaa tgacggtttt
     1381 catataggta tgttcggcca gttcatagta ccggatgcat actttggcct tggcttctat
     1441 acaggctaca aatggttgtc gcgtagagga gtcactttcg agctgggtgc cggtataggc
     1501 agaatcttaa atcctgaacg gagctatggt tactactctg aaactaatat tactggtacc
     1561 ggccaagcta ctgtcggata taggtttggg tatggaaaaa ctcaagtgga gaaaaaaatg
     1621 taacaggttt ataaatgaac cggattgaat cccaatcttt cttcgacgga caaatacagt
     1681 tgctgacagg gctgaatgaa tttagagaat cgctttgggt taatgggttt gccttgcgca
     1741 atcaattaac agatgaagaa atactaccat tcatcagttt ttttgacatc attggtatta
     1801 cacaggtcgg tgatgtactc aatattacat tcaaaattta tcctgacgga ctaaagagct
     1861 ataccataga agtaaacccg ttcttgaaaa ctttcagata caagggcaag gaatatccgg
     1921 tgagttgttt tgaggaaatg tttgtagaga aaggctaatc atcttcattc ggagagtcgg
     1981 gtacttctca gaggatgttc agccattgaa atggcagcat tttcatgtta tgcaacacca
     2041 taatccacac ccatgtactt ttccaatatt cacactacac caaatcaaaa atatccttga
     2101 cgccaaatag tcggttgacc tgaaaacctt cagcaaaggt catattggcc ggacgaccga
     2161 agtctcgtga tctggcagca ttgaactcca aaaaatcttt gccggaaatt ggcgaatcgg
     2221 gttctgctga atcagggtcg taaaactgag agcggaaggt cttaatcagc tcgatttttc
     2281 tgtcgaaata tggcgtaata tcgaatacaa aatcgggcac aagatgataa tcctgtatgt
     2341 aatgcagcac cagctttggc ctccatgctt cctgtggctg tccggtcagc ttgtcgatgg
     2401 tttctatctt cctgagcccg gagtaaaaac aggcatcatc ttcgagtctt gccgaacgcc
     2461 cgtggtcggg gtggcggtcg ctgttgctgt tggcaaacac gatatccggc ctgtaacgtc
     2521 ttataacttc aataatcggc aggatactct ctttgttgat ttcgaaaaat ccgtctgcca
     2581 gatcgagctg ttctctgact gtagcaccca tcagcctggc cgattccatt gcctcctgag
     2641 tgcgaagctg tgcattgcct cgtgtaccga gttcgccctt ggtgaggtca agcaggccta
     2701 cttttttacc ttggtcgatg taatgaagca gcgtaccgac acatgacaat tcaacatcat
     2761 ccggatgtac cccgatggcc agaatatcta atttcataac gattatttca aattaataac
     2821 ttccataata ccttctcata aaccactcgg ccgacaaaag ggcaagtagt aagaagaaca
     2881 gccatttgag gttgatgaca ggctgagtaa tgttcgattg ataaagaact ggctttaacc
     2941 tgtcggatga aacaaccaga ttatgaagtt tgtccatttc atccttatac accatctgtc
     3001 cactagttga ctgcgcaagc aatttcaaca gtccatgatt ggctgtggtt tcgtagagtt
     3061 ccagctgtac cggttgtact tcaaattttc cttcgaatat cagcttgtct cctatgccgg
     3121 aagtcttcgc tacaaaacta tatgcccccg gtggaaaata tcctgcattc aggttaaaat
     3181 attttcctgt cttgctgaat gtgtatggaa actccttacc gttttcattt tttatggtca
     3241 tagatacatc accgtcattg atgagctcat aagaattatt atagacttca gcatcgaagc
     3301 tgatctgttc gttttcaagg aaaatattct taggcattgt caccctgaat ttccttttat
     3361 cttccttcac agatagatac tgaatgctct tatcaatgag ttggtcaatg atattgtgat
     3421 tctgacgctg cagataatca aacagtcgcc acttccagat gccttcacca gccagtattc
     3481 cggtcttgat accattaaat tctccaaaca tcaatagagg ataattggtg tcgattttac
     3541 caattttctg cataagcaaa acgcttgcat tgcctgtaag cttataatca ccaaacatgg
     3601 tagtcaatgg tggaaattgt tcaatggcct ggctgacaga cggatcaagc gtaaaaagac
     3661 caaaattctg aacgagcgac gcctgtgcat cattggtatt gcgcccgtca ccgttaatgg
     3721 tcaacaatga ctgaatttta ttgaatttgt aaatgtccgt ctgcgtaccc acaacaaaca
     3781 aacgtggctt cttggcggca tcgagctgtt gcagaatatt gctgacatca ctgttccttg
     3841 atggcaactg atggagaatg acaaagtcat aatcagtaac tttcttttta aaatccgcaa
     3901 ggagtgaagt ctctaccaca taattttggt tggtagaaat cacctgacgt atggcagaaa
     3961 tatccggatg cgggccattt gccagaatga gtatctttaa cctcgagtcg atgatgtcaa
     4021 cgtaaaaatc cttcacatta ttgacggtgg tcatttctcc atctacttta ctaagactta
     4081 tcctataatg ctgaacaccg ggtttatcgg catcaagcac catatcgacc gatttgaaga
     4141 aatccttgtt gtcgattgtc agattgcctt tttgaagtat ttgggatgtt tttccgtcta
     4201 tcctacttag ttgccaatgt gtagattttc cctgacagtt cctggctgca atatcggctt
     4261 gtatggtaaa tttgtctttc agatatgcaa tatcatggtg gtaaaccttc ttaatataaa
     4321 ggtctttttt ggggatagta tcaccgagtg ctatcggata aaccggaaca ttaatgaggc
     4381 ctgctgtata gccgggatca ctaccctgat tgttgatacc gtctgtggcg agaatgatag
     4441 cacccagatt ctggttgttg tagatatccg ttacagtagt caggacttca gaaatatcag
     4501 ttgatttgtc agaaaatttt tcggcgagac cttcccttga tttttcacca aaactgtagg
     4561 tttttacatc ataatcctta ccaaacgaag atgcaagttg ctttacctgc tcgagatatt
     4621 ttgcctgatc cccgtccttt aaatcagcag ttaccgattc tgatacgtcc tgggcgatca
     4681 cgatgaccgg cttcttgata tcagtttttt ttgacttcaa caccggagca agcaacaaag
     4741 ctgcaagcaa aaacagtacg atgaaacgta gtgctgcaag tacttttcgt agccacattg
     4801 gttgtccctg
//
LOCUS       DAOQQY010000225         4808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8169_length_4808_cov_5.826846, whole genome shotgun sequence.
ACCESSION   DAOQQY010000225 DAOQQY010000000
VERSION     DAOQQY010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8169_length_4808_cov_5.826846"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /locus_tag="PLE23_10055"
     CDS             <1..2025
                     /locus_tag="PLE23_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMX83649.1"
                     /translation="EIAAEVGELLHKIRNLHPIDYEDFSQDDYCQFFKLTQHSDSHEV
                     YKKMAEYDEQDARIERQVQNIVTAKNQPADENENNDGQVEKILVETEEKSEQNSNTKN
                     EIQDQKTESSLLADLKNFKEEHLNEQATVTQPSAGESVNTTEEQLPDTIIISTEEMDK
                     SKNLERELSEKFGKILNDPPGTDATTMYGPTGILQEYRNRLIKDPDNVNLRLELVKLL
                     IEDPDNFSETADHLEHILVIDKENTEAIFLIGRLSESVGEHQLARTYFNKLAALQPDY
                     PGIFVKLAEITEAHFPEEHAELSNYYKKAFDHDTTRIDLLEKRGVLLYKSLNKAKKAK
                     KIFQKLIKWNPLHPTAGYYLADIYFEAGQLEKATETFAKAKEITSRLTTETQTPTAKP
                     STEIPVQEPVTTTPHDKKTSAEKLVTSYVCITGATSGIGKATAIKLASKGNNLILTGR
                     REDRLEDLAEEIEDMYDVQVETLVFDIRNEAETTLAIQSLGEKAENIEILINNAGLAM
                     GLAPIHEGNIQHWNTMIDTNIKGLLFMTRLIAPAMVRKNKGHIINICSTAGKEAYPNG
                     NVYCATKFAVDALTKSFRQDLYKYNIRVSQVAPGAVENTEFALVRFEGDADKAKIYND
                     FNPLKSDDIANIIDFIISQPANVNIQDVLVMATQQASAVLIDRSGRKFDQ"
     gene            complement(2516..3562)
                     /locus_tag="PLE23_10060"
     CDS             complement(2516..3562)
                     /locus_tag="PLE23_10060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HMX83650.1"
                     /translation="MKYRYLLFITILFSFKGEIFCQDPRFSQFYTTPQLINPALTGVF
                     DGEYRINVNYRNQWNSILNKSAFTSYAAGGEYRYNVADEDYVGGGLQFLKQNSGVGDL
                     SRLQTHLNASYIKQLGSNYRGPDYYLAAGLQLGFGQLSTNWNKYWFSRQYDPNSQSVN
                     TNINSGESGADRSGLFLDLGAGLLWYAVFDERTSIYVGGSLGHLNKPDIALYDDSKES
                     MYNKFTAHAGGEFLITPYFSLLPAAYFITQGPSRELVAGSNIRYSNGDWREFSIRAGG
                     FARINNRLNSFNNFESTVVNLSFEFDRWMMGLSYDINFSDLQAVTNSRGAFELSLTYI
                     ARERARYRVKCPTF"
     gene            complement(3665..>4808)
                     /locus_tag="PLE23_10065"
     CDS             complement(3665..>4808)
                     /locus_tag="PLE23_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83651.1"
                     /translation="GGSTPYTFKWNTGATSPLIAALPSGVYNCTITDANACQRNVSAS
                     ISEPEPIKLNGNVINPVCEYDSNGSISLNPAGGNAPYKYQWANGSTTSSITGLTQGNF
                     VLTVTDITGCSITSTFAVKAQNQIESEVEILAAACFEESTGAASINVTEGKAPLQYSL
                     NGQPFSSDNYFEKLPAGKYEATIKDKDGCTDKLQFEIKSGADFTIDKIQDMSVEYGKN
                     LEVTPNIALNGQVSYKWNTINVAKAVCDTCLRLDLTPQQDGYVWLLVTDENGCYKEVK
                     FFIDVIKDYKVFVPTAFSPNNDGVNDYLSIFAKDGIQVAEIKVFDQWGELVYSVDKPE
                     MNQESKGWDGSFYGRPMNTGVFVWVLKAIYPDGSEEILSGETTLIR"
BASE COUNT         1576 a          985 c          875 g         1372 t
ORIGIN      
        1 gaaatagccg ctgaagtggg cgaattgctt cacaaaatcc gaaaccttca tcctattgat
       61 tatgaagatt tctcccaaga tgattactgc cagtttttta agcttactca acattctgac
      121 agccatgaag tttataaaaa gatggcagaa tatgacgaac aggacgcccg tatcgaacgt
      181 caggtgcaaa atattgtcac tgcgaaaaac caacctgccg acgaaaatga aaataatgat
      241 gggcaggtag agaaaatatt ggtcgaaact gaagaaaagt cagaacaaaa ctccaacacc
      301 aaaaacgaaa tacaggatca aaaaacagaa agctccctac ttgccgattt gaaaaatttt
      361 aaagaagaac acttaaacga acaagctact gtaacccaac cttctgccgg agaatccgta
      421 aacactacag aagaacaatt acctgacact atcataatca gtacggaaga aatggataaa
      481 agtaaaaatc tggaacggga actttccgaa aaatttggta aaattctgaa tgatccgccc
      541 ggaactgacg ctacaactat gtacggacca actggcattt tacaagaata ccgcaataga
      601 ttaataaaag atcctgacaa tgtgaacctc cgacttgagt tggtaaaact tctaatcgaa
      661 gatcctgaca acttctcaga aactgcagat cacctggaac acatacttgt gatcgataaa
      721 gaaaatactg aggccatttt cctcattggc agactgtcag aatctgtggg cgagcatcag
      781 ctggcacgaa catattttaa taaactagca gcattgcaac ccgattatcc gggcattttt
      841 gtcaaacttg ctgagataac agaagctcat tttcctgaag aacatgctga actgagtaat
      901 tattacaaaa aagcattcga tcatgataca acacgtattg atttgctaga aaaacgagga
      961 gtgttgctct acaaatcact caacaaagct aaaaaagcaa agaagatatt ccagaaactt
     1021 ataaaatgga atccgctaca cccaacagca ggatattacc tcgccgatat atattttgag
     1081 gctggtcagt tagaaaaagc aacagaaaca tttgctaaag ctaaagaaat aacctcacgc
     1141 ctcactaccg aaacacaaac accaacagcg aaaccaagta ctgaaatacc ggtacaagag
     1201 cctgtgacaa ccacgccaca tgacaaaaaa acatcagctg aaaagcttgt tacaagctac
     1261 gtatgcatta caggtgctac gtcaggtata ggtaaggcta cagccataaa gctggcgtca
     1321 aaaggtaata atttgatact gacaggtcgt cgtgaagacc ggcttgaaga cttggctgaa
     1381 gaaattgaag atatgtacga tgtgcaagtc gaaaccctcg tattcgatat ccgcaatgaa
     1441 gcagaaacca cacttgctat ccagtctctt ggagaaaaag ccgaaaatat agaaatactc
     1501 atcaataatg caggtctggc catgggtctc gcacccatac atgaagggaa tattcaacac
     1561 tggaatacga tgatagacac caatataaag ggattgctct ttatgactag acttattgct
     1621 ccggcaatgg taagaaaaaa taaaggccat atcatcaata tttgctctac agccggcaaa
     1681 gaagcttatc ctaacggtaa tgtctattgt gctacaaagt tcgcagtaga tgcccttaca
     1741 aaatcattca gacaggattt gtacaagtat aacatccgag ttagccaggt ggcacctggg
     1801 gcagtagaaa atacagaatt tgcacttgtc agatttgaag gtgatgcaga caaggccaaa
     1861 atctacaatg attttaaccc attgaaatca gatgacattg ctaatattat tgattttata
     1921 ataagtcagc ctgccaatgt caatattcag gacgtacttg tgatggctac ccagcaggcc
     1981 agcgctgtac ttattgaccg atcgggtaga aaatttgacc aataaggcag ttgaataaga
     2041 aaattaactg tgtttgggac actttttaca gggttttgac tttttgtatt ttttacaaca
     2101 tttcttcttt ttatccttac agcattcaaa cattgtctga agaccctctg tacggctttt
     2161 agttgtcact cctgcaatga tggctattgt atatttcata attcaaaaat atgcacaaat
     2221 atacaaataa ttatttttaa ttagatttat tctaaattag aattttgtta aaaaaatgtc
     2281 gtgttcacat tccaatttga cctatatcaa taaaataaat attaaaattt tactcgaagt
     2341 gtacgatatc tagaacaagt tagttagctt tgtgctattg agaaaaccaa aacaattact
     2401 atgttgttat tatacatcgt attactgata tttttaatcg taggcatatt gaccgtttcc
     2461 agcaaacatg gccgatttat caattaaata acgctcatca agctaatttt ttatatcaga
     2521 atgttgggca tttgaccctg tatcgtgctc tttcgcgtgc tatgtatgtc aatgacaatt
     2581 cgaaagcgcc tcttgagttg gtaactgcct gtaggtcaga aaaattaata tcataactga
     2641 ggcccatcat ccatctgtca aactcgaaac tgaggtttac tactgttgat tcaaaattat
     2701 taaaactatt caaacgatta ttgatgcggg caaaacctcc ggctcgtatt gaaaactctc
     2761 tccagtcgcc attgctgtaa cgaatgttgg agccggctac cagctcgcga gaagggcctt
     2821 gtgtgatgaa ataggctgcg ggcaacaatg aaaaataagg tgttatcaga aattcgccac
     2881 cggcatgagc agtaaactta ttgtacatac tttccttgct atcgtcatac aatgcaatat
     2941 caggtttatt aagatgacct aatgaaccac ctacataaat actggttcgc tcatcgaata
     3001 ctgcatacca caacagaccg gcacctaagt caaggaaaag tccagatctg tcagcacccg
     3061 attcaccact gttaatattt gtattcacag attgtgagtt agggtcatat tgtcttgaaa
     3121 accaatattt gttccagttg gtgcttagct gtccgaatcc tagttggagt ccggcagcca
     3181 gataataatc gggtcctcga taattactac ccaactgctt aatataggac gcattgagat
     3241 gtgtctgcaa ccttgacagg tctcctaccc cagaattttg cttcagaaac tgcaatccac
     3301 caccaacata atcttcatca gctacattgt atctgtattc accaccggcc gcatagcttg
     3361 taaatgcact tttattaaga atgctgttcc attggtttct gtaattgaca ttgatgcggt
     3421 attctccatc aaatacgcca gtcaaagccg gattgatgag ttgtggtgtg gtgtaaaact
     3481 gtgaaaaacg cggatcctga caaaagattt cacctttgaa ggaaaataat atggtaatga
     3541 ataataaata tctgtatttc atagttttta tcttgatcaa atgaagacta aagataatct
     3601 tagttttgca aaaataactt tgaaaatttg aattttatta aaaattcttc aatacagata
     3661 ttccttaacg tataagtgtt gtctcgccac tcaatatttc ttctgatcca tcagggtaaa
     3721 ttgcctttaa aacccataca aacactcctg tattcatcgg tcgtccgtag aaactgccat
     3781 cccatccttt tgactcctga ttcatttcag gtttatcgac actatagacc aattcgcccc
     3841 actgatcaaa aaccttgatt tcagctactt gaatgccgtc ttttgcaaaa atgcttaggt
     3901 aatcgttaac accatcgtta ttaggagaaa atgctgttgg tacaaatact ttatagtcct
     3961 tgataacatc gataaagaat tttacttcct tataacaacc attctcatct gtgacaagca
     4021 accacacata tccgtcttgt tgaggtgtta agtcaagtct caggcaagta tcacataccg
     4081 cttttgctac gtttatggtg ttccacttat aactcacttg gccatttaat gcaatattgg
     4141 gtgtcacctc aagattctta ccgtactcta cagacatatc ctgtatttta tctatggtaa
     4201 aatcagcacc tgacttaatt tcgaactgta gcttgtcggt acaaccatct ttgtctttaa
     4261 tcgtagcttc atacttaccg gctggtaatt tttcgaagta gttatcagag ctgaatggct
     4321 gtccattcaa tgaatattgt aatggggcct tcccctcggt tacattgata gaggctgcac
     4381 cagtactttc ttcaaaacag gcagctgcta atatttcaac ttcagattca atttgattct
     4441 gtgctttcac agcaaatgtg ctggtaatac tacaaccggt aatatcagtt actgtaagca
     4501 caaagttacc ttgtgtaaga cctgttatgg aagatgtagt agagccattc gcccactgat
     4561 acttgtaagg tgcattacca cctgccggat ttaatgatat actaccattg gaatcgtatt
     4621 cacaaaccgg attgatcaca ttgccattca acttgatggg ttcgggttca gaaatagagg
     4681 cagacacatt tcgctggcat gcatttgcat ctgtaatagt acaattatat acacctgaag
     4741 gcagtgcagc tatcaatggt gaagtagctc cggtattcca cttaaaagta tatggcgttg
     4801 accccccg
//
LOCUS       DAOQQY010000226         4771 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8258_length_4771_cov_6.054283, whole genome shotgun sequence.
ACCESSION   DAOQQY010000226 DAOQQY010000000
VERSION     DAOQQY010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8258_length_4771_cov_6.054283"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="PLE23_10070"
     CDS             complement(<1..643)
                     /locus_tag="PLE23_10070"
                     /inference="COORDINATES: protein motif:HMM:NF013161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMX83652.1"
                     /translation="MKYFHSLKLTEVRKETPDAVVLTFEVPAELRKEFTFTPGQHLTL
                     KTTIDNEEVRRSYSICAAPFEDTLSVAIKKIDNGIFSTYANTKLSKGHQLDVMLPMGK
                     FSPKLNSSNNKNYMLIAAGSGITPVISIVKSILTQEPKSKVTLLFGNRNTRSVMFKEE
                     LEALKNQYLDRFNLHFIMSREKLDAPILNGRIDKHKLSLFFKYLTDPTSIDEVF"
     gene            845..2050
                     /locus_tag="PLE23_10075"
     CDS             845..2050
                     /locus_tag="PLE23_10075"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMX83653.1"
                     /translation="MDSITHIVVGATAGYMVAGRTYGKRAMIWGALGGSMPDIDVISN
                     LWTDPVDSLMAHRGITHSLLFLAFGSVLLTWLNGLFYKNDLQNGRNMRFFVASFLLVL
                     AAVTALVMNVLLLSFDGLWLLLSIPFCIWGGVKLIKIFRNYVLQPNIFVKPDFRIVWL
                     VFFVSMSTHLFLDTCTVYGTGLLEPFSNHRFALSNISVVDIFFTIPAVVIILLACVTH
                     RGIYYTKINIAWMILYFALTFFNYQYMLDVFGRALDKKDIPVKKMLISPAIMNNFLWY
                     SVAETDTAFVTCSHSLFGQASVPDPINILPKSYQLLPEAASSSQLNTLKAFSRGYYNL
                     VIDKQGDVQWNDLKFGMFSDAPKDANDYLFKFKLRHDNGVWTAQKASEMNKSLAEFWP
                     EYWKRVWGQ"
     gene            2164..2236
                     /locus_tag="PLE23_10080"
     tRNA            2164..2236
                     /locus_tag="PLE23_10080"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2197..2199,aa:Met,seq:cat)
     gene            2292..3503
                     /locus_tag="PLE23_10085"
     CDS             2292..3503
                     /locus_tag="PLE23_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HMX83654.1"
                     /translation="MKKRILQIVLLAFLPLLLHAQEFRVIGYLPQYKISFIDKICIEY
                     LTHLNIAFANPDSQGIIKTFKNDIKPAVERAHYYNVKALISLGGGHLLPEWKTAWTKY
                     LQPSNRSYFISNIMYYVRENRLDGADVDLEWKDVDKNYSGFILELRDSLHAAGKLITA
                     ALPGKHRYANISDEALHAFDFINLMAYDACGPWNPHRPGQHSSIEFASKCIAYWTEKQ
                     GMAPGKLSLGLPCYGYNFNDPSKVYGFYYRSMLERQLEYMNADQVGGMYYNGIPTIKS
                     KTQMAFEKTGGVMMWELSQDAVDSLEEYSLLKAVYETVQVNRELIKEDFSIRVYPNPH
                     THFVYIESNKAEEEAEINLFNQQGILILNKRQKLDVPVIHLETKDLKSGKYPLTVKIG
                     NTVQMHTIIKS"
     gene            complement(3508..>4771)
                     /locus_tag="PLE23_10090"
     CDS             complement(3508..>4771)
                     /locus_tag="PLE23_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HMX83655.1"
                     /translation="ECPDCQNRMKEQGLKTEEELQSYFIHRMEKFLNSKGKQIIGWDE
                     ILEGGLAPNATVMSWRGIDGAIAAAKEKHYAIMTPGTHCYLDHYQSLSLNEPIAIGGY
                     TPLMKVYDFEPIPSELTTDQQKYILGAQGNIWTEYILNEAHVEYMAFPRLCALAEVNW
                     TPRERKNEADFKKRLIHHLLRLKAMNVNFAKHVFDPEVTTSANEKAELEITLTPMKAS
                     DIIRYAINHEPTDQDPIYTKPVTLTSASTFYCRAQGEVQGSLLSVTFYKSASFGANIT
                     LKHAASDRYPGAKGAATLVDGLKGGKRYDGQNWLGFSGDDMEAVIDLGSKKSISEVAV
                     GSMDIKGSWIHPPKRLQVYTSNDGIDYKIVSDASAPLDFDGIAHSVTFKEEARYVKVI
                     AQNQGIIPDGFQGAGHKAWLFIDEIMVK"
BASE COUNT         1289 a          963 c         1019 g         1500 t
ORIGIN      
        1 taaatacttc atcaatagat gttgggtcgg tgaggtattt gaagaacaaa ctgagcttgt
       61 gcttgtcgat tcgtccgtta agaatgggag catccaattt ttcgcgtgac ataatgaagt
      121 gtagattaaa ccggtcgaga tactggtttt ttaatgcctc gagttcttct ttgaacatga
      181 cagacctcgt atttctgttg ccaaaaagga gtgttacctt actctttggt tcttgtgtca
      241 ggatactttt tacaattgaa atgaccggag taataccact acctgctgca atgagcatgt
      301 aatttttatt atttgaacta ttgagttttg gtgaaaattt gcccatcggc aacatgacat
      361 caagttgatg tcctttgctg agtttagtat tggcataggt tgaaaatatg ccgttgtcta
      421 ttttttttat tgccacactg agagtgtctt caaatggtgc tgcacagata gagtatgagc
      481 ggcgtacttc ttcgttgtca atggtggtct tcagcgtaag gtgttgtcct ggtgtgaagg
      541 taaattcttt ccgtaactct gccggtactt caaatgtcaa gacaacagca tccggtgttt
      601 cttttcttac ctcggtcaat ttcagtgaat ggaaatattt catctggaca aatatttttt
      661 ccaaagataa aaaattacca tcattggtgt acttttaaag caataaactt ttccgaattt
      721 ttctcaaata attcgaattc ctgatgatgg tctgtgcaac aattgtacca tacacagcaa
      781 acattactgt acatatttca ccattatctt tttacctctg cttatcttgt gccaaaattg
      841 ataaatggat tcaataacgc atatagtggt tggcgccaca gctggctata tggtggcagg
      901 gaggacatac ggcaagagag caatgatttg gggtgctttg ggtggctcaa tgcctgatat
      961 tgacgtaatc tcaaatctct ggacagaccc ggttgactca ctaatggcgc atcgtggtat
     1021 tacgcattca ctactttttc tggcatttgg ttcggtttta ttaacttggc tcaatggtct
     1081 gttctacaaa aatgatcttc agaacggtcg gaatatgcgt tttttcgttg caagtttcct
     1141 gcttgtattg gccgcagtta cagctctggt tatgaatgtg cttttgctga gttttgatgg
     1201 gttgtggttg ttactgagta ttcccttttg tatttggggt ggagttaaat tgataaaaat
     1261 attcagaaat tatgttctgc agcccaacat atttgtcaaa ccagatttta gaattgtatg
     1321 gctggtcttt tttgtttcga tgtccacaca tttgtttttg gatacttgta cagtttatgg
     1381 taccggattg cttgaaccat tctccaatca ccgttttgca ctaagcaata tttcggttgt
     1441 ggatattttc tttaccattc cggcagttgt tatcatactg cttgcatgcg ttacacatcg
     1501 tggtatttat tatacaaaaa tcaatatagc ctggatgata ttgtactttg ctcttacttt
     1561 ttttaattac caatatatgt tggatgtgtt cggccgcgca ttggacaaaa aagatattcc
     1621 tgtaaagaaa atgctgatat cgccagccat catgaacaat tttctttggt attctgtggc
     1681 agaaaccgac actgcatttg tcacttgcag ccattcgcta ttcggccagg cgagcgtacc
     1741 cgatccgatt aatattctgc ccaaatctta ccaattattg cctgaagcgg catcaagcag
     1801 tcaactgaat acgctcaagg cattctcccg gggatattac aacctggtta ttgacaagca
     1861 aggcgatgtc cagtggaatg atcttaagtt tggtatgttc agtgatgcgc caaaagatgc
     1921 gaatgattat ttatttaaat ttaaacttcg ccatgataat ggtgtctgga cagcccaaaa
     1981 ggcaagtgaa atgaataaat ctcttgcaga attttggccg gaatactgga agcgagtctg
     2041 gggtcaatga taaataagct cacaaatcat tgaatatcag gtgttgttgc tttatttgaa
     2101 tatattttta tgataaaatt tggatatata gtacagttgg atgtatcttt gcactcacaa
     2161 tatcgcgggg tggagcagtt ggtagctcgt cgggctcata acccgaaggt cgtaggttcg
     2221 agtcctgcct ccgctacaaa gagaaagccg gtcaaacttt tgatcggctt ttgtatttaa
     2281 gtcctggcca aatgaaaaaa cgaattttac aaattgtact attggcattt ctgccattgt
     2341 tgctgcatgc tcaggagttc agggtaatcg gttatctgcc gcaatacaag ataagtttta
     2401 ttgataaaat ctgtatagaa tacctcacac atctcaatat cgcatttgcc aacccggata
     2461 gtcagggcat catcaaaaca tttaaaaatg acataaagcc tgctgttgaa agagcgcatt
     2521 attataatgt gaaagctttg atatccttag gtggcggaca tttgttgcca gaatggaaaa
     2581 cagcatggac caagtatctc caaccttcca acagatctta tttcataagc aatatcatgt
     2641 actatgtacg agaaaaccgg ctcgacggtg cggatgttga cttagagtgg aaggatgttg
     2701 acaagaatta cagtggcttt attctggagc tacgcgattc attgcatgca gcaggcaaac
     2761 tgataactgc agctttgccc ggcaaacaca gatatgccaa tatctcggac gaagcgctac
     2821 atgcttttga ttttatcaat ctgatggcct atgacgcctg cggtccgtgg aatccgcata
     2881 ggccaggtca gcattcttca atagaatttg cctcgaaatg tattgcgtac tggactgaaa
     2941 agcaaggcat ggcaccaggg aaattatctc tcggacttcc gtgttacgga tataacttta
     3001 acgacccaag taaagtgtat ggcttctatt accgctctat gcttgaacga cagcttgagt
     3061 atatgaatgc cgaccaagtc ggtggtatgt attataacgg cataccgaca atcaaatcta
     3121 aaacccagat ggcattcgaa aaaacaggtg gcgtaatgat gtgggaactc agtcaggatg
     3181 cagttgattc tcttgaagaa tattcgctac tgaaggctgt atatgaaacc gtacaggtca
     3241 atagagaact aattaaggaa gatttcagta tccgcgttta tcccaacccg catacacatt
     3301 ttgtctatat cgaatcaaac aaagcagaag aggaagccga aatcaatctc ttcaatcaac
     3361 aaggaatact tatactcaat aaaaggcaaa aacttgatgt gccggttatt catcttgaaa
     3421 ccaaagacct aaaatcaggc aaatatccat tgaccgtaaa aatcggcaac accgtgcaaa
     3481 tgcacaccat aatcaagagc tgaagcttta ttttaccata atttcatcta tgaacaacca
     3541 agctttgtgt cctgcaccct gaaacccatc cggtattata ccctgattct gtgctattac
     3601 tttcacgtac cttgcttctt ccttaaatgt gacagaatgg gcgatgccgt cgaagtcaag
     3661 gggtgccgaa gcatctgata cgatcttata atcgatacca tcattgcttg tatatacctg
     3721 cagacgtttt ggcggatgta tccacgaacc tttgatgtcc atactgccga ctgctacttc
     3781 actgatgctc tttttactgc cgaggtcgat aactgcctcc atgtcgtcac cgctgaaccc
     3841 aagccagttt tgcccatcgt agcgtttgcc acccttcagg ccatccacta atgtagcagc
     3901 acctttagct cccggataac ggtctgaagc agcatgctta agtgttatat tggcaccgaa
     3961 tgaagcagat ttgtaaaaag taacggataa taacgatccc tgtacttcac cctgggctct
     4021 gcagtagaat gtagatgcag acgttagggt aaccggtttg gtatagatcg ggtcttggtc
     4081 agttggctca tggttgatgg catatctgat gatatccgaa gccttcatgg gcgtaagtgt
     4141 tatctcgagc tcagcttttt cgtttgcaga tgttgtcact tcggggtcaa aaacgtgttt
     4201 agcgaaattg acattcatcg ccttaagcct caacaaatgg tgaataagcc gttttttaaa
     4261 atctgcctca ttcttgcgtt cgcgtggggt ccagtttact tctgccagag cacaaagtct
     4321 cgggaaagcc atatactcga catgtgcttc attgaggatg tattcagtcc agatattgcc
     4381 ttgtgctcca agtatgtatt tttgttgatc ggtagtgagt tcggatggta ttggctcaaa
     4441 atcatatact ttcatcagtg gtgtatatcc accaatggct atgggttcgt tgagggagag
     4501 tgactgataa tgatctaagt aacaatgtgt gccaggtgtc atgatggcat aatgtttttc
     4561 tttggctgcc gctattgccc cgtcaatacc tcgccatgac ataactgtgg cattcggtgc
     4621 aagaccgcct tcaagtattt cgtcccagcc tattatctgc ttgcctttgc tgttcaggaa
     4681 tttttccatc ctgtggatga aatagctttg cagttcttct tctgtcttca gaccttgttc
     4741 cttcatcctg ttctgacagt ccggacattc t
//
LOCUS       DAOQQY010000227         4760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8285_length_4760_cov_6.123698, whole genome shotgun sequence.
ACCESSION   DAOQQY010000227 DAOQQY010000000
VERSION     DAOQQY010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8285_length_4760_cov_6.123698"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..765)
                     /locus_tag="PLE23_10095"
     CDS             complement(373..765)
                     /locus_tag="PLE23_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMX83656.1"
                     /translation="MSTHAKNPFTWVEIYVADMQRAQKFYETVLQISMNPMQTPGDFG
                     DLEMVSFPWSQDGPNITGALCKTKEMQPGTGGTLVYFACDDCSVEYSRVEAAGGKVLQ
                     EKFQIGEHGYCAIVMDTEGNTIGLYSMR"
     gene            complement(794..1279)
                     /locus_tag="PLE23_10100"
     CDS             complement(794..1279)
                     /locus_tag="PLE23_10100"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HMX83657.1"
                     /translation="MKNLKIEVKWAIIFSMVALCWMVLEKVSGLHGKYIDYHLYLTNL
                     FAIPAIIVMVKALKEKKSVFYSGNMSYKQGFTSGTILSIFIALLSPLTQWITTYIITP
                     EFFPNVIKRSVETGYYKTMAEAEAFFNYQNYAQQGMIGALVMGIITTAIAMLFLRSKN
                     I"
     gene            complement(1396..1827)
                     /locus_tag="PLE23_10105"
     CDS             complement(1396..1827)
                     /locus_tag="PLE23_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMX83658.1"
                     /translation="MLSLSCKAAIKSVIYLGSISDTARNAGIKEIASYINENEHTVGK
                     LLQKLVKGHVIHSIKGPNGGFFMTPEQKNQPIINIIQTIDGKEVFHHCGLGLSKCNAS
                     HPCPLHNDFKPIRDNFERMCYEKKINDLYENVNKGLAYLIG"
     gene            complement(1894..3192)
                     /locus_tag="PLE23_10110"
     CDS             complement(1894..3192)
                     /locus_tag="PLE23_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83659.1"
                     /translation="MFPIASTDAIKTTSYKIVLPFYIYAAVWFLIAAVMMFVRAEAFM
                     NHFFHPHLLAITHIMALGWATMVILGAAHQLVPVLIEGKLHSDRLAYATFILAALGIP
                     LLVYGFYHFEMHAPAKWGGRFVILAVLCFLINIYKSMAKSKSENVHAVFVFTAILWLF
                     ATVAFGLALVYNFTYTMFPHDYLHYLPLHAHTGIIGWFLLLVIGVGSRLIPMFMISKY
                     TNPKLLWIIYLLINGALVIYLIIFYFFNKPGITMVPSLLIMLAVVLFIKYCYHAYKER
                     LRKTVDEQIKISLLSVAMLLIPMIMLVILIGVFMTSASEKEKLSLVICYGFVIFFGWL
                     TAIILGMTFKTLPFIVWNKVYHLQSAKGRTPNPKDLFSDVIFRIMSISYLAGFVIFGS
                     GILSEKSILLQSGATLLIVAAFFYNWNVVKVITHKASIPS"
     gene            complement(3194..4000)
                     /locus_tag="PLE23_10115"
     CDS             complement(3194..4000)
                     /locus_tag="PLE23_10115"
                     /inference="COORDINATES: protein motif:HMM:NF021520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HMX83660.1"
                     /translation="MIINSNTKIAAILKENPDALEAIVSISPRFTKLRNPFLRKLMAS
                     RTSISMASKVGGCSVHDFFEKLKPLGFTVDDTADASDEVKQEAIPDFLRKVAKSDIID
                     LDVRAMLEAGNDPFNLIREKLNTLGKGQVLKIINTFEPTPLIHLMSKQGFESYTEVYD
                     KDEIFTYFYRGDEIRNFDPEIKKQNDDWDSVLLKFEGKLVTTDVRQLEMPMPMLTILE
                     LLENLPEDNALYVYHKRIPVFLLPELADRKLDYRIKEISDGEVHLLIFKP"
     gene            complement(4049..>4760)
                     /locus_tag="PLE23_10120"
     CDS             complement(4049..>4760)
                     /locus_tag="PLE23_10120"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX83661.1"
                     /translation="CLIEASPRLLGALSEKSAERVRNYLTKLDVEVLTDTSVIDYDGT
                     TVVLNNGDKLVSNCLIWAAGVKGFSFEGMPEQVQAPNGRLKVNGFNQLEGNENIFVIG
                     DAAIMSSPSLPKGHPQVAPVAIQQATLLAKNLECIVKSQPMESFEYHDKGSMATVGRN
                     KAVAEIGKIRLTGFFAWLTWMAVHLMSIIGVKNRLVVLLNWMWQYFTYDQSLRLIIKA
                     EEKKITGNTSKNTAGMPV"
BASE COUNT         1352 a         1015 c          926 g         1467 t
ORIGIN      
        1 cagggaaaag aaaataatta tttcaacaac ctgaacgcca catttactga aaggcatggc
       61 aatgagataa gtgtaaggtt tatttatttc ctcgactaca atcggctgaa gaagcagaag
      121 atcaaaggtt aaatccagat agcgcattag gttcttgact gcaaataatt atttctccaa
      181 atccttttca aaagctcaaa aatcatagtt cacaatgctt ttattacttt tttattgatt
      241 ttttacacga atcatttgcg ctcaattggc taatgcgtta tccggattaa aacagtagta
      301 gttgttgcct cttgcttaga acaataaaaa gtattttatc ataatataaa caagagtgca
      361 ggtgtgcacg catcatctca tagaatacag accgatggtg ttaccttccg tatccataac
      421 gatggcgcaa tagccgtgtt caccgatctg gaatttttcc tgtaggacct tgccaccagc
      481 cgcttctact cttgaatact ctaccgagca gtcgtcgcaa gcgaaataaa cgagtgtgcc
      541 acctgtccct ggttgcattt ctttggtctt acacaaagca cctgtgatgt tcggcccatc
      601 ctgcgaccat gggaagctta ccatttcgag gtcgccaaaa tcgcccggtg tctgcattgg
      661 gttcatactt atctgcaata cagtttcata aaatttctgt gcacgctgca tgtcggcaac
      721 gtagatttct acccaggtga aagggttttt agcatgagtt gacatggtat atttatttaa
      781 atgggttaac agttcagata ttttttgaac gaagaaaaag catagctata gctgtagtga
      841 tgatacccat taccaatgct ccaatcatac cttgttgtgc ataattctga taattgaaaa
      901 acgcttcagc ttctgccata gttttgtaat acccggtttc taccgaccgt tttataacat
      961 ttgggaaaaa ttcaggcgtg atgatgtagg tggtgatcca ctgtgtgagc ggactgagca
     1021 aagctatgaa tatagaaagg atggtaccgc tggtaaagcc ttgtttatag ctcatgttgc
     1081 cactataaaa cacgcttttt ttctctttta atgctttgac catcacaatt attgccggta
     1141 tggcaaacag attggtcaag tacagatggt aatcaatgta tttgccatgg aggccgctga
     1201 ctttctccaa taccatccag cataacgcta ccattgaaaa aatgatagcc cattttactt
     1261 caatttttag gtttttcata gtgtttgttt ttgaaaccta aatttattac ttattatcgg
     1321 aattacccaa atttatttat ctgtactcat atattacttc atcatttttc tattttttgc
     1381 ctggtcaacc aatgtttatc ctatcagata ggcaagacct ttattgacgt tttcgtagag
     1441 atcgtttatt tttttttcat aacacattct ctcaaaatta tcgcgtatgg gtttaaaatc
     1501 attgtgtaaa gggcaaggat gagaggcatt gcacttactc agcccaagac cacaatggtg
     1561 gaaaacctcc ttgccatcaa ttgtttgaat aatattgatg atgggttggt ttttttgttc
     1621 tggcgtcatg aagaatcctc cgttgggtcc tttgatgctg tgtatgacgt ggcctttaac
     1681 gagtttttga aggagcttgc ccacggtgtg ttcattttcg ttgatatacg aagcgatttc
     1741 cttgatacct gcattacggg ccgtgtccga tatcgaacca aggtagatga ccgatttgat
     1801 agctgcttta caacttagac ttaacatata tttcagattg atggttaaac aatattttgt
     1861 gccgcttact catcctgtgc ttttttgttt gtgtcaggat ggtattgaag ctttgtgtgt
     1921 tattactttc actacattcc aattataaaa aaacgctgca actatcaaaa gcgttgcgcc
     1981 tgattgtaac aaaattgatt tttcgctcaa tatgcctgac ccaaatatta cgaaacctgc
     2041 cagatatgat atactcatga ttctaaaaat cacatcactg aagaggtctt tcgggttagg
     2101 tgttctgcct ttagcagatt gaagatgata gaccttgttc caaactataa aaggcaatgt
     2161 cttgaaagtc ataccaagaa taatagctgt gagccaacca aaaaatatga caaaaccata
     2221 gcagataacc agacttagct tttctttctc agaggcagaa gtcatgaata cacctatgag
     2281 tatcaccagc ataatcatag gtatgagcaa catggctact gacaacaaag atatctttat
     2341 ctgctcatca acggtctttc tgagtctttc cttgtacgca tggtaacagt atttgatgaa
     2401 caataccacg gccaacatga tcaaaagcga tggtaccatg gtaatgcccg gcttattgaa
     2461 gaaataaaat atgatcagat aaatgacaag tgccccgttt atcagtaaat aaataatcca
     2521 caacagcttc gggttggtat atttcgatat catgaacata ggtatcaacc gcgaacctac
     2581 gcctattacc agcagcaaaa accagcctat aatgcctgta tgtgcatgta gtggcagata
     2641 gtgcaggtaa tcgtgcggaa acatcgtgta tgtaaaatta taaaccaggg ccaggccaaa
     2701 ggccaccgta gcgaaaagcc aaaggatagc cgtaaataca aagacagcat gtacgttctc
     2761 tgacttactt ttcgccatac tcttgtaaat atttatcaaa aagcacaata cggccaatat
     2821 cacaaatctt ccgccccatt ttgcaggtgc atgcatctca aagtgataaa aaccatatac
     2881 caataaggga atgccaaggg cagcaagtat aaatgtggca taagccagtc tgtcactgtg
     2941 tagtttacct tctatcagta cgggtaccag ttggtgcgca gcgcccagta tgaccattgt
     3001 agcccagcca agtgccatga tgtgggtgat ggcaagcaga tgaggatgaa aaaaatgatt
     3061 cataaaagcc tctgccctga caaacatcat aacagcagct atcaaaaacc agaccgcagc
     3121 atagatgtaa aacggtagta ctatcttata tgatgtggtc tttatggcat ctgtactcgc
     3181 tatcgggaac atattaaggc ttgaatatta aaagatgaac ttctccgtcg cttatttcct
     3241 ttattctgta gtcaagcttc ctgtctgcaa gttcgggaag caaaaaaacc ggtatcctct
     3301 tgtggtaaac atacagcgca ttgtcttcgg gcagattttc aaggagttca agaatggtca
     3361 gcataggcat aggcatttcg agttggcgaa catcagttgt tactaatttt ccttcaaatt
     3421 taagtaaaac tgaatcccag tcgtcatttt gtttttttat ttctggatca aagttccgga
     3481 tttcatcccc gcggtaaaaa tacgtaaata tctcatcctt atcatatact tcggtatatg
     3541 actcaaaacc ctgcttcgac ataaggtgga tgagtggtgt cggctcaaat gtgttgataa
     3601 tcttcagtac ctgccctttg cccagtgtat tgagcttttc acggatgagg ttgaatgggt
     3661 cattgcctgc ctcaagcatg gcgcgcacgt cgaggtcaat gatgtcagac tttgccacct
     3721 ttcgcaaaaa atccggaatt gcttcctgct ttacttcatc tgatgcgtct gctgtatcat
     3781 caactgtaaa gccaagtggt tttaattttt cgaaaaaatc atgtacgcta cagccaccta
     3841 ccttgctcgc catactgata gatgtacgcg atgccatcaa cttgcgcaaa aacgggtttc
     3901 tcagtttagt aaaccttggc gagatactca cgatggcttc cagagcatcc ggattttctt
     3961 tcagaatggc tgcaatttta gtattactgt tgataatcat taacttctgt tcgattttgt
     4021 tgaataaatt gccctttgat ttcctacatc ataccggcat tccggcagtg tttttgctgg
     4081 tgttgcccgt aatcttcttt tcttctgcct tgatgattaa tctcagcgac tggtcgtatg
     4141 taaaatattg ccacatccag ttgagcagca cgaccaacct attcttgaca ccaataatcg
     4201 acatcaagtg aacggccatc caggtaagcc aggcaaagaa tccggtaagc cttatcttgc
     4261 ctatttctgc tactgcctta ttccttccaa cggtagccat acttcccttg tcatggtact
     4321 cgaatgattc cataggctgt gacttgacta tacattccag gtttttggca agtaaggtcg
     4381 cttgctgtat ggccacaggc gccacctgag gatgtccctt cggcaatgaa ggtgatgaca
     4441 tgatggctgc atcacctatc acaaaaatat tttcgtttcc ttcaagctgg ttaaatccat
     4501 ttaccttcaa ccttccgttg ggtgcctgta cttgttcggg cataccttca aatgaaaaac
     4561 cttttacacc tgctgcccat ataagacagt ttgataccaa cttgtcgcca ttgttcagta
     4621 caacggtcgt tccgtcataa tcaattaccg atgtatccgt cagtacttct acatccagct
     4681 tggtcaggta atttctcaca cgctcagccg atttttcact caatgcgcca agcagccttg
     4741 gtgaagcttc tatcaggcat
//
LOCUS       DAOQQY010000228         4698 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8445_length_4698_cov_4.781391, whole genome shotgun sequence.
ACCESSION   DAOQQY010000228 DAOQQY010000000
VERSION     DAOQQY010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8445_length_4698_cov_4.781391"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2637
                     /locus_tag="PLE23_10125"
     CDS             <1..2637
                     /locus_tag="PLE23_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX83662.1"
                     /translation="FIGHAHVAYIPSGKVIGLSKLNRIVDYFAKRPQVQERMTVQIAN
                     ELMEVVIKAAKAPLTIRGDTIEYDASTFKVPPGSTVEDLLRRLPGIEVGQDGSIKSEG
                     RDVTKVTVDGKKFFGADPKAATKNLPAEGITKVQIFDEETEEKKLTGVSSTMPDKVMN
                     LALKDEFKKGSFGKVTASGGTENTYEFKGNINKFNDKEQFSIIGNVNTTGRNGLSWND
                     YQDFKGSGADRWDDGGTFGFGSGRRWFSISFAGDDDDDSGGDSFFSEASSGFPKNYKG
                     GANYNFDNNKNQATATYFYSRTGLYSDNYKNGKTFLPDATLNNIDHTTNDKLNGNHEV
                     NLSLDTKIDSLSSVKINLDGNFVNQNKTNKGLLSLLRQEGDEKYVTNTTFLNNSSENS
                     AFISRLNAIYRKKFRKKGRSFGISSGVSYSKNDRNATQNSTNSFFNESGAQDSVISIN
                     QLHRTLSDRKTIDANTMYTEPLSKKFFWSTFYNFNYLTEKVDRDVTDVLNGSDKKNDF
                     LSRYYNNDIMTNRLGTFLRYSWQGTNISLGIARQRLDLSGNYESGPSAGIKGTVDKVF
                     YNWLPNFNMSFDLRGNKYINTSYSVDASAPSIKRLQPIVDNSNPLYITVGNPDLKPEI
                     GHSVSIGFNKFNPANFIRLSLHSGFTYTEDQIISYQQVTSSNVTTSRFINYSGGQNLW
                     TWTSFGFPIIKNKFTVNLSYNPGYSRSFAFVNNVLNKTNSFSNNGSVRINITPDENLS
                     IYLNASLGHTDTKYNINSSQNQQTLNQNYNLEFNTKLFWGIFLNTSFSYNKFENQRYG
                     FDRDIPLLNLSLYKFVLKGNKGEVRVSLYDAFNKNEDINQYTGFNTVTQSSTTALARY
                     AMLSFSYNIRGMKANKESNFWW"
     gene            2651..3406
                     /locus_tag="PLE23_10130"
     CDS             2651..3406
                     /locus_tag="PLE23_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HMX83663.1"
                     /translation="MIMFKKFIAFIVFSVLSTGFLSAQGFEGEIVYERTYFWVNVNQH
                     LPYLSEEEKDRMKLTYGKKAGWSSKYKLWAKDNQTLYTYDEDQPVESDWAWRQDEFVI
                     FRDLNKRSTKQLIETLGRTYLIDEAATPPKWKILNEIKDVAGYICMKAETYDSIKDQR
                     IVAWFTDQIPVDGGPEMMFGLPGMILELNMNQGSVIITALSVKPMKLEKGLPLPKKWK
                     GKKIENEAFQEQIKKHITESIAAKRNPYWQIRY"
     gene            complement(3460..4242)
                     /gene="kdsA"
                     /locus_tag="PLE23_10135"
     CDS             complement(3460..4242)
                     /gene="kdsA"
                     /locus_tag="PLE23_10135"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HMX83664.1"
                     /translation="MSKFKLIAGPCAVEERDTCYAIAGHLKRMAEKYELDLIFKASYK
                     KANRTKLNSFTSIGEGQALQILEDIRKEFSVHTITDIHESTDPERIAAHVDYFQIPAF
                     LCRQTDLLINAGKYAPKGVNIKKGQFLSPEMMQFAAEKVRFGNPNTDVLLTERGTTFG
                     YEYLITDMTSFPKMSAFGTTIMDCTHSVQIPNQSSGITGGDARMIGTMAKAAIAAGAQ
                     GLFIEVHPEPSKASSDAGSILQMDKLEEILRVCVRIYDAVRQ"
     gene            complement(4247..>4698)
                     /locus_tag="PLE23_10140"
     CDS             complement(4247..>4698)
                     /locus_tag="PLE23_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HMX83665.1"
                     /translation="GQIALNIATTFSSTGSCAVYLHPSEAQHGDLGVLRPEDVLLLLS
                     NSGKTREIVELLELSKRMYPDIPAIVITGNAQSELAQNAEIVLFTGGAPEICPLGLTP
                     TTSTTVMTIIGDMLVVGLMEKIGFTKEEYAKRHHSGYLGSKSRGEIH"
BASE COUNT         1445 a          921 c         1007 g         1325 t
ORIGIN      
        1 ttcatcggac atgctcatgt cgcctatatt cctagcggca aggtgattgg cctgagcaag
       61 ctgaaccgca ttgtggacta ttttgccaag cgtccgcagg tacaggagcg catgaccgtg
      121 cagatcgcca atgagctgat ggaagtggta atcaaggcag cgaaagcacc gcttacaata
      181 cgtggtgata ccatagagta cgatgcttct accttcaagg tgcctccggg atcgactgtg
      241 gaagacttac taagacggct gccgggtatt gaggttggtc aggatggctc tatcaaatcg
      301 gaaggcaggg acgtcaccaa agtaacggtt gatggtaaga agtttttcgg ggctgaccca
      361 aaggctgcta ctaagaatct acccgctgaa ggaatcacca aagttcagat atttgatgaa
      421 gagactgaag agaaaaagct cacgggtgtt agctctacta tgccggataa agtgatgaac
      481 cttgccctta aagatgagtt caagaaagga agttttggaa aggtgactgc aagtggtggt
      541 accgaaaaca cctacgagtt caaaggcaat atcaataaat tcaatgacaa agagcagttt
      601 agcatcatag gtaatgtcaa cactacagga cgcaatggct tatcctggaa tgattaccag
      661 gactttaaag gttcgggcgc tgatagatgg gatgacggtg gcacatttgg ctttggcagt
      721 ggcagaagat ggttttctat aagttttgca ggagatgatg atgatgattc tggtggcgac
      781 agctttttta gcgaagcatc atcgggtttt ccgaaaaatt ataagggcgg tgcgaattac
      841 aatttcgata ataataagaa ccaggcgact gcaacctact tttatagccg cacaggattg
      901 tactcagata actacaagaa cggaaagaca tttctacccg atgctacact gaataatatt
      961 gaccatacca caaacgacaa actgaacggc aaccacgaag tgaatctaag cctcgacacg
     1021 aaaattgatt ctctaagttc agtaaagatt aatctcgatg gtaattttgt caatcagaat
     1081 aaaacaaata aaggactctt gtcattgctc aggcaggaag gtgatgaaaa atatgttacc
     1141 aacactacat ttcttaataa cagctccgaa aacagcgcat ttatttcaag attgaatgcc
     1201 atttacagaa aaaaattcag aaaaaaaggc agaagcttcg gtatcagttc tggtgtgagt
     1261 tatagcaaga atgacagaaa tgccacacag aattccacca atagtttctt caatgaatca
     1321 ggtgcacagg attctgttat ttccatcaat caactgcacc ggacattatc tgaccgcaag
     1381 accatagatg ctaataccat gtacaccgag ccactcagta agaaattttt ctggtcaacc
     1441 ttctataact ttaattatct aaccgaaaag gtggacagag atgtgacaga tgtactcaat
     1501 ggaagtgaca aaaagaatga tttcctttca agatattaca acaacgatat catgaccaac
     1561 aggttgggta cattcctgag atattcctgg cagggcacta atatttcact gggtattgca
     1621 cgtcagcgcc ttgacttgag tggaaattat gaatcaggcc cttcggcagg tattaaaggg
     1681 acggtcgata aagttttcta caactggttg cctaatttta atatgagttt tgaccttaga
     1741 ggtaataaat atatcaatac aagctatagt gtagatgctt ctgcaccttc catcaaaaga
     1801 cttcaaccga tagtggacaa cagcaacccg ctctacatta cagttggtaa tccggacctc
     1861 aagcctgaga ttggtcattc ggtaagtatc ggattcaata aatttaatcc tgcaaatttt
     1921 atcagattgt cactacatag cggatttaca tatacggaag accagatcat ctcataccag
     1981 caggttacga gtagcaatgt cactacatca cgttttatca attatagcgg aggacaaaat
     2041 ctttggactt ggacaagttt tggttttccg attattaaga ataaatttac ggtcaacctg
     2101 agttacaacc ctggttactc caggagtttt gcctttgtca ataatgtgct gaacaaaaca
     2161 aattccttca gcaacaatgg ctctgtgcgc atcaatatta caccagacga aaacctgagt
     2221 atttatctta acgcctcatt ggggcatacg gacaccaaat ataatattaa ttcaagtcag
     2281 aaccaacaga ccttaaatca aaattacaac ctggagttca ataccaagct attttggggc
     2341 attttcctca atacttcttt cagttataac aagtttgaaa accaaaggta tggttttgac
     2401 agagacatac ctttgttgaa cttaagtttg tataagtttg ttctcaaagg gaataaaggc
     2461 gaagtcaggg tgtcactata tgatgctttc aataaaaatg aagatataaa ccaatataca
     2521 ggctttaata ccgttacaca gtcttctact acagccttag ccaggtatgc aatgttaagc
     2581 tttagttata acataagagg tatgaaggcg aataaagaat caaatttctg gtggtaaaat
     2641 ttttttgtaa atgattatgt ttaaaaagtt tattgcgttc attgtttttt cagtattatc
     2701 aacaggattt ctttcggcac aaggttttga aggggaaata gtctatgaac gcacctattt
     2761 ttgggttaat gtcaatcaac atcttccata cctgagcgaa gaagaaaaag accgtatgaa
     2821 gctgacttat ggaaaaaagg ccgggtggtc gtctaaatac aagttgtggg caaaagataa
     2881 tcaaacgcta tatacatacg atgaagatca gccggtggag tctgactggg cgtggcgtca
     2941 ggacgaattt gtgatttttc gtgatttgaa caaaagaagc acgaagcagt tgatcgaaac
     3001 acttggcaga acctacctca tcgacgaggc tgctacacct cccaaatgga aaatactgaa
     3061 cgagataaaa gatgtggccg gatatatctg catgaaagcc gaaacctacg atagtatcaa
     3121 agatcagcgg atagtagcct ggtttactga tcagattccg gtagatggcg gacccgaaat
     3181 gatgtttggc ctaccgggta tgattctcga gctgaatatg aatcagggta gtgtcattat
     3241 cacagctctt tctgtaaagc caatgaaact tgaaaaagga ctccctttgc caaaaaaatg
     3301 gaaggggaaa aaaattgaaa atgaagcatt tcaggaacaa attaagaaac acataacaga
     3361 atcaattgct gcaaaaagga acccatattg gcagatacgg tattgatgtt gagctcgttt
     3421 ttgttatgac ggcatttagc cgattggcag atgatgtagt tactgtctca cagcatcata
     3481 tatacgcaca catacacgga gtatttcttc cagtttgtcc atttgcagga tacttccggc
     3541 atcggagctt gctttggaag gttccggatg cacctctatg aacaagcctt gtgctcctgc
     3601 tgctatggct gctttagcca tggtgccaat catacgagca tcgccacctg ttataccact
     3661 actttggtta ggtatttgta ctgagtgtgt acaatccatt atggtcgttc caaaagctga
     3721 catttttgga aaagaagtca tgtcggttat cagatattcg tagccgaatg tggtacctcg
     3781 ttctgtaagc agtacgtctg tgttcggatt gccaaaccgt actttttcag cggcgaattg
     3841 catcatttct ggcgaaagaa actgaccttt cttaatattt acacctttag gtgcatattt
     3901 gccggcattg atgagcaggt ctgtctggcg gcacaaaaat gcaggtatct gaaaatagtc
     3961 aacatgtgca gctattcgtt cagggtctgt actttcatgt atatctgtga tggtatgtac
     4021 cgaaaattct ttcctgatat cttcaagtat ttgcaatgct tgcccctctc cgatagatgt
     4081 gaaactgttg agtttggtac gatttgcttt cttgtacgaa gctttgaata tcaggtcaag
     4141 ttcatacttt tctgccattc tcttaagatg cccggctatg gcataacaag tatctcgttc
     4201 ttcaacggca cacgggccgg ctattaattt aaattttgac atatatttaa tgtatttctc
     4261 ctctgctttt tgatcctaaa tatccagaat ggtggcgttt tgcgtattct tccttcgtaa
     4321 agccaatttt ttccatcaaa cctactacca acatatcgcc aataatggtc atgacagtag
     4381 tggaagttgt aggagtgaga cctaatggac aaatctcagg tgcaccaccc gtaaacaata
     4441 ctatttctgc attttgagct agttcgcttt gggcattgcc ggtaatcacg attgcgggaa
     4501 tgtcgggata catccttttt gacaactcca gaagttcgac tatttctctg gtttttcccg
     4561 aattggataa aagtaaaagt acatcttcgg gccttagtac gccaagatca ccatgttgcg
     4621 cctcacttgg atgaagatat accgcacaac tgcccgtaga actaaatgtc gtagcaatgt
     4681 ttaaagctat ctgtccgg
//
LOCUS       DAOQQY010000229         4648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8577_length_4648_cov_6.396255, whole genome shotgun sequence.
ACCESSION   DAOQQY010000229 DAOQQY010000000
VERSION     DAOQQY010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8577_length_4648_cov_6.396255"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PLE23_10145"
     CDS             complement(<1..1316)
                     /locus_tag="PLE23_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMX83666.1"
                     /translation="MKQNFHSPTRLSEGVIARLSYFVRSFILFILLFAGLEMAAQPCV
                     MKCKPGPIQLTLDGGCSKLIDPNSLLDLPQTTCTGSKTGIAFNGGGNPIGNVITSTFL
                     GQTLNVTVLDIDSGQSCMTSISVVDTTKPILICQSDITISCNKSFAVPDLPAPIVIDN
                     CGGSVSLTNTDVIIDSLCPKPATVFRTWKAVDASGNSKTCITKITLARPKLTDVEFVF
                     ATNFIIPCSQNPEDLNFMGQPTISGNPIDFNDLCDFEAIKTDSIAPAVAPLTGTTYFR
                     TWAVSDLCLDTTITALQVFTVPDETAPTIVCPADVTFEATQFFCPNPISLPPPASITD
                     DCVALPPLTVNWAFPDMGLGPYSNLPSGVYDVEYVVSDFAQNTAACNFKVTIIDTIAP
                     VAVCKGQVVVALSNIAPGILLPSSVDDGSHDNCTIIKYELDRLDDA"
     gene            complement(1681..3051)
                     /locus_tag="PLE23_10150"
     CDS             complement(1681..3051)
                     /locus_tag="PLE23_10150"
                     /inference="COORDINATES: protein motif:HMM:NF018269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HMX83667.1"
                     /translation="MKNKAFYIILLVCILFVSCKKDNQGPVEDGDVNAGGSTTVDVSF
                     VNTFQQPAPNLTAAEIELHRQGDLAFGDIFVTAPSVVNSGLGPVFNQNSCESCHVSNG
                     KSSFPLSTDNTKGLLIRLSMPGTGAHGGPLPVPGFGGQLQTKAVYGKTAEAQLNWQEI
                     EEIKSFVDGEIYALRRFEFSYNNPYIPWPTGVLTSPRIAPAIIGLGLLEAISEADLLA
                     NADPDDKNADGISGRPNYGWNEQTQQKQIGRFGWKAAQPTLIQQSAAAYNNDMGITNP
                     IFTKESSFGQLQYDKLNDDPEIDMNTLKVAAFYTQSLGVPKRRNWADAEVRQGKTLFT
                     KIQCDACHKPKFVTGQHPEYPFLSNQTIYPYTDLLLHDMGEALADNRPDFEASGREWR
                     TPPLWGIGLTKTVGSHTNFLHDGRARNLEEAILWHGGEAEKSKEAFRKMSKTERTALI
                     RFIESL"
     gene            complement(3054..4106)
                     /locus_tag="PLE23_10155"
     CDS             complement(3054..4106)
                     /locus_tag="PLE23_10155"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HMX83668.1"
                     /translation="MIKKTPIFPLVFALLFTACKKDDIVPTTDYQEVLSDVSYNVILN
                     TYQQLELKTASLAGSLSVLKNTPTASNLEAARQAWRDARDPWEQSEGFLFGPVDQKGI
                     DPSIDSWPVNQTDLDAVLNSSNALTKAYIDGLDGTLKGFHTIEYLLFGENGNKLVGDF
                     TARQFEYLDGCSQSLLGATQALNDSWREDKDNFISYIINAGKAGNTIYPSQKSALEEL
                     MYGMITIADEVGNGKINDPFSQQNVDLEESRFSKNSKKDFADNIRSIKNIYLGSYNNK
                     SGKGMSTIIVGKKAQLDAKLISEIDAAIVAIENIQGSFTEAIFNAKQSVTDAQQKVRA
                     LQQTLESEALPIISNL"
     gene            complement(4124..>4648)
                     /locus_tag="PLE23_10160"
     CDS             complement(4124..>4648)
                     /locus_tag="PLE23_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83669.1"
                     /translation="NTTYNRPKPDLHIATPVGLGEFHFRYKNALINVSGMCMYGVLGN
                     SEALSNQNRNLSNALNVKRTPVGAAALGAYLEAGITVLGKIGLIKSLKVGDCIVFGRF
                     DYYDTMYKTQGLIFNNPRWERKTITGGLVYKIIDDIHFKVQYSVRKVGAPAPTSIHGG
                     TLENTFIGGFAFEF"
BASE COUNT         1219 a          979 c         1002 g         1448 t
ORIGIN      
        1 tcagcatcat cgagccggtc aagttcgtac ttgataatcg tacagttatc atgactgcca
       61 tcatctacag acgacggaag cagtatgccc ggagcaatat tggacaatgc gacaaccact
      121 tgccctttgc agacagctac cggagcaatt gtgtcaatta ttgttacttt gaaattgcag
      181 gctgctgtat tctgtgcaaa atcacttact acatattcta catcatacac tccggatgga
      241 agattactgt aaggcccaag ccccatgtct gggaatgccc agttgacagt aagtggaggc
      301 agcgctacac aatcatcggt aatactcgcc ggtggtggca atgatatcgg gttcgggcag
      361 aagaattggg ttgcttcaaa ggtaacatct gcggggcaga caatagttgg tgcggtttca
      421 tcaggtactg taaatacctg caacgctgta atggtagtat ctaagcataa gtcactgact
      481 gcccatgtac ggaaataggt agtgccggta agcggagcaa ctgccggagc aatactgtcg
      541 gtttttatag cctcaaaatc gcacaagtca ttaaaatcaa tgggattacc actaatggtt
      601 ggctgcccca taaaattaag gtcttccgga ttctgactac aaggtatgat gaagttggta
      661 gcaaatacaa actctacatc tgtgagcttc ggccttgcaa gtgttatttt tgtaatgcac
      721 gtcttcgaat tgccggaggc atcaacagcc ttccaggttc tgaatacagt tgcaggtttc
      781 gggcacaagc tatcaattat aacatcggtg ttggtaagtg aaacagagcc tccacagttg
      841 tcaataacta taggcgccgg caaatcaggt acagcaaatg atttgttgca gctaatggta
      901 atatcactct ggcaaatgag tatcggtttg gttgtatcaa ctactgaaat gctcgtcata
      961 caactctgcc cggaatcaat atccaagact gtcacattta gtgtctgccc gagaaaagta
     1021 gaagtgatga cattgcctat cgggttgcca cctccgttaa acgcaatgcc ggttttgcta
     1081 cctgtgcagg tggtctgtgg taagtcaagc agcgaatttg ggtcgataag cttcgaacat
     1141 cctccgtcaa gtgttaactg aatagggccg ggcttgcatt tcatcacgca tggctgagct
     1201 gccatctcaa ggccggcaaa tagtaaaatg aataaaataa agcttcttac aaagtaagat
     1261 aatcttgcta tcacaccttc agatagccgt gtcggggagt ggaaattttg tttcatcctg
     1321 cggtttttgg ttttctaata aatgttacaa aacagacaca catccggaga tgttgccatt
     1381 acggttaagg tgtttttggg ttgattgact ataatgtgga aaacaggaat ctaaataggt
     1441 gagaccggtt ttctcttctt tcagtatgtt gtaataaaat attcctgacc aggctttggt
     1501 cgtggtgtga gttttggtga gaatgttgaa atattacaag aaagaataat gccaatttta
     1561 gttaattggc atttaatcgg accagagttg tccacaaata tcagataatc agcactatat
     1621 acatgctgtt aattttttct ttctttttga gtgaaataga aagtatctat aaaaatttaa
     1681 ctacaggctt tcaatgaatc gaattaatgc ggttctttcc gttttactca ttttcctgaa
     1741 tgcttctttg cttttttcgg cctctcctcc atgccatagt atggcttctt caagattacg
     1801 cgccctgccg tcgtgcaaga aattggtatg gctgcctaca gttttggtaa gtccgatacc
     1861 ccaaagtggt ggtgtgcgcc attctcttcc actggcttca aaatcaggcc tgttatcagc
     1921 aagagcttcg cccatatcgt gtagcagcag gtcggtgtaa ggataaattg tctgattact
     1981 taggaatggg tattccggat gttgccctgt aacgaatttg ggcttgtggc aagcatcgca
     2041 ttgtattttt gtaaaaagtg ttttaccttg tcttacttcg gcatctgccc agtttcttct
     2101 cttaggcact ccaagtgatt gggtgtagaa tgcagctact ttcaacgtgt tcatgtcgat
     2161 ctccggatca tcattgagtt tgtcatattg gagctggcca aaactgcttt ccttcgtaaa
     2221 aatcgggttg gtaattccca tgtcattgtt gtatgcagca gcactttgct gtatgagcgt
     2281 aggttgagct gctttccatc caaatcttcc tatctgtttt tgctgagtct gttcattcca
     2341 gccataatta ggccttccgg atattccgtc agcgttttta tcatccgggt cagcattagc
     2401 aagcaagtct gcctcgctga tggcttccag caagcccaat cctataatgg caggagctat
     2461 ccttggtgaa gtaagcactc cggttggcca agggatgtaa ggattgttgt aagagaattc
     2521 gaaccggcgc aatgcatata tttctccatc tacaaaactt tttatttcct caatttcctg
     2581 ccagttcaat tgtgcttcag ctgtttttcc atatactgct ttggtctgta gctggccacc
     2641 aaaacccgga acaggaagcg gtcctccatg tgctcctgtt cccggcatgc ttaatcttat
     2701 taacaggcct tttgtattat ctgtgctgag tgggaatgac gatttgccat tagatacatg
     2761 gcagctttcg catgaatttt gattgaatac gggtccgaga cctgaattaa ccacactggg
     2821 tgcagtcaca aaaatatcac caaaggccag atcgccttgt cggtgcaatt caatctcagc
     2881 tgcggtaaga ttgggtgccg gttgttgaaa ggtattgaca aaagaaacgt caactgtagt
     2941 actgccacca gcgttaacat ctccatcctc caccgggccc tggttgtctt ttttgcaact
     3001 tacaaacaaa atacatacta acagaataat ataaaaggcc ttgtttttca tttttataaa
     3061 tttgaaataa ttggcaatgc ttctgattcg agtgtttgct gtagtgctct tactttttgt
     3121 tgagcatctg tcacactttg ttttgcatta aagatggctt ctgtaaaact accctggatg
     3181 ttttctatgg caacaatggc agcatctatt tcggaaatga gttttgcatc gagttgtgct
     3241 tttttaccaa caatgatggt tgacatccct ttacccgatt tgttgttgta actacccaga
     3301 tatatatttt tgatacttct tatgttgtca gcaaaatctt ttttagaatt tttactaaaa
     3361 cgactctcct ccaggtcaac gttctgctga ctgaacggat cgttaatttt accattacct
     3421 acttcatcag ctatggtaat cataccgtac attagttctt caagagcaga tttttgtgaa
     3481 gggtagattg tatttccggc tttacctgca tttattatgt atgagatgaa attgtccttg
     3541 tcttcacgcc agctgtcgtt tagtgcctgg gtggccccaa gcagactctg tgaacatcca
     3601 tcaagatatt caaactgtct tgctgtaaaa tctccaacca acttattgcc gttttcacca
     3661 aacaacagat attcaatagt atggaaacct ttcaatgttc cgtccagacc atcaatataa
     3721 gccttggtta aggcatttga gctattgagt acggcatcca ggtcagtctg gtttaccggc
     3781 cagctgtcaa tggatggatc gatacccttc tggtcaaccg gaccaaatag gaagccttct
     3841 gattgctccc aaggatctcg ggcatcacgc catgcttgtc gggctgcttc aagattggat
     3901 gcagtagggg tgttcttcaa tacacttaag ctaccggcaa ggcttgcagt cttaagctca
     3961 agctgctgat aagtgttaag gattacgtta tatgatacgt ccgaaagtac ttcttgataa
     4021 tcagtagtag ggacaatatc atctttttta catgctgtaa acaacagtgc aaaaacaagc
     4081 ggaaagattg gcgttttttt gatcatgata aaattttggt ttattaaaat tcgaatgcaa
     4141 atccacctat aaatgtattt tcaagtgtac caccgtggat gctggtagga gcaggcgcac
     4201 ccacttttct cacagaatat tgaactttga aatgtatgtc atctattatt ttatatacca
     4261 acccgcctgt tatagtttta cgctcccaac gtgggttgtt gaatatgagt ccctgggttt
     4321 tgtacatagt gtcgtagtag tcaaatcggc caaaaactat gcaatctccg acttttaggc
     4381 tttttataag accgatttta cctaacactg ttatgcctgc ttctaagtaa gcacctaagg
     4441 ctgcagctcc tacgggagtc cgttttacat ttagagcatt cgacagattg cggttttgat
     4501 tgctgagtgc ctccgaattg ccaagtaccc cgtacatgca catcccgctt acattgatta
     4561 aagcattttt atacctgaaa tgaaattcgc ccaagcctac cggagtggct atgtgcaggt
     4621 caggtttggg cctgttgtag gtagtgtt
//
LOCUS       DAOQQY010000230         4604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8705_length_4604_cov_5.709387, whole genome shotgun sequence.
ACCESSION   DAOQQY010000230 DAOQQY010000000
VERSION     DAOQQY010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8705_length_4604_cov_5.709387"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>4604
                     /locus_tag="PLE23_10165"
     CDS             <1..>4604
                     /locus_tag="PLE23_10165"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83670.1"
                     /translation="TAPAPVGEQTISFVWDDWHRSNIASTPAGSASGTAIVNNTTPSD
                     CYVVRNVTIRSVLPMSASKTTISCPADFMAGTVNISTLIMGGYNVEYKLFDNGVLKAT
                     NKSGIFNIPNTFIGPVTVVVSDGSGCSPNITININNNCMKAPACPNPNGEKINGGTIP
                     VTVCANENFTLSVDGSTSLNLPSGGTIDWYYSKTTPFNPYAGQGTYFGKGNITGTPPV
                     PPKDCATFSAGDIAIVAYQATNTDIFAFVALTDIAPGAQIGFTDNGWRNTNVFRTGEG
                     VVRWTAPASIVPAGTVITITPSTPSATLGTVVKETGSLDFAAGGDQLIAFCGTFNTST
                     GAISGIVLTGVHFNGSSWDADATSASTSALPLGLTNNQNAVALGKFSNGKITCSGGTI
                     TGSTTVVSAMINTATSWTLSNSVLAPPVSTCSYNITGLAGTTMVSDLTTSFGPDMCNM
                     LLYIKGIVRPFPGAPCSETDASTVTFELNVNCPTAVLSGGGTACAPGSVPLTIDFGNY
                     TGSPIFTINYTIDGVAQASIMTNSDPYVLNASTPGEYELTSVSAPGNCMFMVSGSAMV
                     SINPAPDVNLNGSITVCPGTIAVVPITINSGTLPFTIEYTLNGVPQAPVEIFSNVLNI
                     PIPEGTPAGPYTVVITSLTDDNQCAGTSSGSFTIIVQTIPPDSAPMSDQNYCYDNSGS
                     LNVNVNLPLLPAGLTADWYDAEVGGNLVLANSNNLVTSITPTGPYPQTFTFWREIKNT
                     MTGCVSLNRVPVNIIVNLAPDLVTPTSQSNACPATTVDLSLLSITDNNGTTGSFTYHA
                     TLADANSGMNPISSVISVSGTYYIRKTTSAGCFDVVPVVVNIITCVSCPTISTVSTSG
                     NLCANSNFTVTASGLANMDMGSNGEANFGIEFKAFTSIPADPYTGGTSLGVVPFGSLM
                     SGNTIATLNNASIGAGTYYIYAILSPASSTPGCTPSAFTQVTVNSAPSLSESYIAPSC
                     PGSADGSIDLTVTGNGGYTFNWATSGGSGLNPGSEDQSGLTAGTYNVTVTDVNGCTGS
                     LSIILPQATDNQPPVITCPADITIQCSASTLPASTGSATATDNCSVQSITYTDVTNLN
                     GCGGYTGTITRTWRAQDGSGNTATCIQTITLIDNSAPSFNPPLPADMTIQCADMIPTA
                     PVITATDNCDASVTVSFNETTTPGTCPNRMTITRTWTATDDCNNSTQYIQVLTIHDDT
                     PPVFTAPLPANITVSCESIPDTADLTATDNCSGGGGPATPVIWINEIHYDNTGADVNE
                     FVEVAGTAGIDLSDYSLVLYNGSNGQSYNTLPLSGTLPNQSNGYGAAAFVYPPNGIQN
                     GAPDGVALVHNPSSTVVYFLSYEGSFTATNGPANGQTSVDIGVLETGTEPAGMSLSLT
                     GTGSDYPDFAWQSPATSTPGTINSGQMMVSSGTPAIITYTFTESSLAGSCPNEKTITR
                     TWTATDECGNSATHQQIITVIDDTPPTFDQPLPADVTVSCDAVPLAPELTATDNCNAP
                     GGTTPVIWINEIHYDNTGADVNEFVEVAGTAGIDLSDYSL"
BASE COUNT         1262 a         1036 c         1008 g         1298 t
ORIGIN      
        1 tacagcccca gcaccggtag gcgaacagac tatcagcttt gtttgggatg actggcatcg
       61 ctccaatatt gccagtacac ccgccggtag tgcatctggt acggcaatag ttaataatac
      121 aactccttca gattgttatg ttgttcgaaa tgtaacaata cgtagcgttc tgccaatgtc
      181 ggcaagcaaa acaactataa gttgcccggc agactttatg gccggtacag ttaacatcag
      241 tacgcttata atgggtgggt ataatgttga atacaaacta tttgataatg gagttctgaa
      301 agcaaccaat aaatcgggga tttttaatat acctaatact tttataggcc cggtaacggt
      361 tgttgtatct gatggtagcg gctgttcgcc gaatattact attaatatta acaacaactg
      421 tatgaaggca cctgcatgtc ctaatccgaa tggcgaaaaa atcaatggag gtacgatacc
      481 ggtaacggtg tgtgcaaacg aaaattttac actatctgtt gatggtagca ccagtctgaa
      541 tttaccttca ggaggtacca tcgattggta ttacagcaaa actacaccgt tcaatcctta
      601 tgcagggcaa ggtacctatt ttggaaaggg caatattaca ggaacacctc ctgttcctcc
      661 aaaagattgt gctacatttt cagccggaga tattgccatc gttgcttacc aagccaccaa
      721 tacggatatt tttgcttttg tggctttaac ggatattgca ccaggagcac agattggttt
      781 tacggataat ggctggagga ataccaatgt tttccgtacg ggagaaggag tcgtgaggtg
      841 gacagcacct gcatcaattg tacctgctgg tactgtgata accatcaccc catcaacgcc
      901 ttctgctacc ttgggtacag tggttaaaga aacaggcagc ctagattttg cagcgggcgg
      961 agaccaactt attgcatttt gcggtacttt taacacttct accggtgcta tcagtggaat
     1021 tgtcttgacc ggagttcatt ttaatggcag ttcatgggat gcagatgcaa ctagtgcaag
     1081 tacatcagca cttccacttg gtcttaccaa taatcagaat gctgtagccc tgggtaaatt
     1141 cagcaatgga aaaattactt gcagtggcgg aactattacc ggtagtacaa cagttgtatc
     1201 tgcgatgatt aatacagcaa ctagctggac tttgagcaat tcagttcttg ctccccctgt
     1261 atctacctgt agctataaca ttaccggatt ggctggaaca acaatggttt ctgaccttac
     1321 cacatccttt ggccctgata tgtgcaacat gcttttgtac atcaagggga ttgttcgacc
     1381 ttttcctggt gcaccttgtt ctgaaacgga tgcatccacc gttacgttcg aattaaatgt
     1441 gaattgtccg acagcagtat tgtcgggcgg tggaacagca tgtgctcccg gctctgtccc
     1501 tttaacaatt gactttggta actacacagg tagtccaatt tttacgatta attataccat
     1561 tgatggcgta gctcaggcat ctatcatgac caattctgac ccatacgtat taaatgcttc
     1621 cactcctggc gaatatgagc tgacaagcgt gtcggcacca ggcaactgta tgtttatggt
     1681 gagtggatct gcaatggtta gtataaatcc cgcacctgat gtaaacctta acggtagtat
     1741 tacggtttgt cctggaacga tagctgttgt tcctattacc atcaacagcg gtaccttgcc
     1801 atttaccata gaatatacat tgaacggagt gcctcaggct ccggttgaaa tcttcagtaa
     1861 tgtactgaac atacccattc cggaaggaac tcctgccgga ccttataccg tggtgatcac
     1921 ttcattgacg gatgataacc aatgtgccgg aacatcttct ggttctttta ccattattgt
     1981 gcagacgatt ccaccagatt cagcacctat gagtgaccag aattattgtt atgacaacag
     2041 tgggtcgttg aatgtaaatg ttaacttgcc tctattgcct gcaggcctta cagcggactg
     2101 gtatgatgca gaagtagggg gcaaccttgt actagcaaac agcaacaatc ttgttacgtc
     2161 aataactcct acaggacctt atccgcaaac cttcactttc tggagagaaa tcaaaaatac
     2221 aatgactggg tgtgttagtc ttaatcgcgt acctgtcaat atcatcgtta acctcgctcc
     2281 ggatctggta acacctacca gccagagcaa tgcatgtcct gcaaccactg ttgatttatc
     2341 tttactttct atcacagaca ataacggtac aaccggttca tttacatatc atgctacact
     2401 ggcagatgca aattcaggaa tgaatccgat ttcgtctgtt atttcggttt ccggaacata
     2461 ttatataaga aagacgacct cagcaggatg ttttgatgta gtaccggtgg ttgtgaatat
     2521 cattacctgt gtcagttgtc caactatttc tacagtatcg acctcgggca atctctgcgc
     2581 aaattcgaat ttcacagtta ccgcaagcgg attggccaat atggatatgg gttcaaatgg
     2641 tgaggccaac ttcggaatag aatttaaagc atttacatca atacctgctg atccttatac
     2701 aggcggaaca tctttgggtg ttgtaccatt tggttcgctt atgagtggta atacaatcgc
     2761 tacacttaat aatgcgtcta taggagcggg tacatattat atatatgcta ttctttcacc
     2821 tgcttcttct acacctggtt gtacgccatc tgcctttaca caggtgacgg taaactcagc
     2881 cccttcgctt tcggaatcat atattgcgcc ttcttgcccg ggttctgctg acgggtcaat
     2941 cgatcttact gtgacaggta atggcggcta cacattcaat tgggccacat ccggagggtc
     3001 aggtctaaac ccgggctctg aagatcagtc aggacttact gctggaacgt ataatgttac
     3061 agttactgat gtcaatggat gtaccggttc gttatccatc attttaccac aggcaaccga
     3121 caaccaaccg cctgttatta cttgtcctgc agatattacc atccaatgca gtgccagtac
     3181 tttgccggca tcaacaggtt ctgctacagc aaccgacaat tgttctgttc agagcattac
     3241 ctatactgat gttacaaatt tgaatggttg tggcggttat acaggtacca tcaccagaac
     3301 atggcgggca caggatggca gcggaaatac agcaacctgc atccagacga ttacattaat
     3361 agataattcg gcaccaagtt tcaatccgcc tttgccggca gacatgacca tacagtgtgc
     3421 ggatatgatt ccaactgccc ctgtcattac agctactgat aattgtgatg cttcggtgac
     3481 tgtaagtttt aatgaaacta ctacaccggg aacttgtccg aaccggatga caataacacg
     3541 tacctggacg gccactgatg attgtaataa cagtacacag tatatccagg tgctaaccat
     3601 tcatgacgat actccaccgg tatttaccgc accacttcct gcaaatatca ccgtatcatg
     3661 tgaatcaatt ccggatactg ccgatttgac ggctacagat aattgttcgg gaggtggagg
     3721 cccggctaca ccggttatct ggataaatga gattcattat gacaatacag gtgcggatgt
     3781 caacgaattt gtagaagtag ccggtactgc aggaatcgat ctttcggatt attcattggt
     3841 attgtataat ggttcaaatg gccagagtta caatacattg ccactatccg gtaccttgcc
     3901 aaatcaatca aacggatatg gtgcagcagc atttgtatat ccaccaaacg gaattcagaa
     3961 tggtgcccct gatggtgttg cactggttca taatccttca agtaccgtag tttacttcct
     4021 gagctacgaa ggttctttta cggctactaa tggcccggcg aatggtcaga cgagtgtaga
     4081 tattggtgtt ttggagactg gaacagaacc tgccggaatg tccttgagtc ttacgggaac
     4141 aggatccgat tatccggatt tcgcatggca gtcacctgca acttcaactc cgggaacaat
     4201 taattcagga cagatgatgg taagttccgg tacaccggca ataatcacct atacatttac
     4261 agaatcaagt cttgcaggta gttgtccgaa tgagaaaaca atcacaagaa catggactgc
     4321 cacggatgag tgcggtaatt cagccaccca ccagcaaatc attactgtaa tagatgacac
     4381 accgcctaca tttgaccaac cattgccggc tgatgtcacc gtgtcttgtg atgcagttcc
     4441 acttgcccca gagcttactg caactgacaa ttgtaatgca cccggaggta ctacaccggt
     4501 tatctggatt aatgaaatac attatgacaa tacaggtgcg gatgtcaatg aatttgtaga
     4561 agtagccggt actgcaggaa tcgatctttc ggattattca ttgg
//
LOCUS       DAOQQY010000231         4543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8871_length_4543_cov_4.831551, whole genome shotgun sequence.
ACCESSION   DAOQQY010000231 DAOQQY010000000
VERSION     DAOQQY010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8871_length_4543_cov_4.831551"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLE23_10170"
     CDS             <1..924
                     /locus_tag="PLE23_10170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83671.1"
                     /translation="TPSGLAASSITSSSATMSWAAVSGANSYDLQYKTNSSGTWTTVN
                     TTSTSSTLTGLTSGTTYNTQVRAVCSSGPSAYSAQVNFTTASAGCTDNYESNNSKTNA
                     KTIATGTNINALISSSSDVDWFKFTTTSSKKNVKVNLSNLPADYDMKLYRSSTLVATS
                     QNSGTQDEQCISNNTKTATFYVYIYGYSGAYDPSNCYNLYVTVSGSNFRTNGDEEWTE
                     LTRVGDELLVWPNPAQNEINVRLPFGEEEVSGDLNIFDATGKFIYQQKLTGSKENSDY
                     RIDVSSFSNGLYFINWKTLSNNLVQKVIVTK"
     gene            complement(1093..1266)
                     /locus_tag="PLE23_10175"
     CDS             complement(1093..1266)
                     /locus_tag="PLE23_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83672.1"
                     /translation="MKKHYLFLSVVLIAMSLFNLTLDIDSRGHYFGNGIVCGLGLSML
                     VIQLKKWNQTTKV"
     gene            1534..2478
                     /locus_tag="PLE23_10180"
     CDS             1534..2478
                     /locus_tag="PLE23_10180"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMX83673.1"
                     /translation="MQVVCSSDYLFRKNSEGKEQSIYYTIYAPASTIVRATLLVLHGM
                     QEHSGRYKELGEYFAQNGFAVLTYDHLGHGNTAKNLSEHGYFQMQNPVGQLVEDTSLM
                     AEKLHTQYPNVPHFILGHSMGSFIARAFLQQHSETFTGSVIVGTGGKIPGIGIAKWCM
                     AILNRLIPHRRSTFVNNTFSRFNSIRFRKDPDYSPISWLSLNPFNRKNFEADRLNGIP
                     FSFNGYHTLVSVNKRATEHEWAHNIRRDFPMLYVSGSEDPIGNFGRGVLQTTNELIRQ
                     GFSNIAVKLYPKMRHEILNEEIRQDVFAEILQWLETKM"
     gene            2745..3191
                     /locus_tag="PLE23_10185"
     CDS             2745..3191
                     /locus_tag="PLE23_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83674.1"
                     /translation="MLSDKTGKNDVIVRVDKKVKVKTINKETIKGKITYIKDSTIIIG
                     EKSPREINIGDIKYFYVRKADGWKTATATLLALTAVESTIFLIQAIESNQKTPPNQGT
                     IDNPVTGLIGLGAITIPATYLGTYYGFFHKKKYDTRLRYALRIIKP"
     gene            complement(3261..4025)
                     /locus_tag="PLE23_10190"
     CDS             complement(3261..4025)
                     /locus_tag="PLE23_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83675.1"
                     /translation="MARKIPDENRYLKILTDTLDITFRNDDKILIESSKTLRLVIGIL
                     GMALPLFLWLSLYLFSGHCEPLDSISHYFFTKASTFFIGIISVLAIFFMIYKGYDSTD
                     FFVSSLAGLAAILLLLLPTGNIVQDLEESHDYIVTVWTYVHPQSDYRQVAHFIFAALF
                     LGSLAYMSICQFTKSDLHPGERKSEKILRNRMYRVFGSIMILALVLIFLGTKGYIMPV
                     ESYNRYHLTFWMEAVAVESFGISWLIKGGAICKDKS"
     gene            4289..>4543
                     /locus_tag="PLE23_10195"
     CDS             4289..>4543
                     /locus_tag="PLE23_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83676.1"
                     /translation="MNTINPKFLSPDRFINRHLGPSEEEAAEMLKLIGYDDVNALIHD
                     TIPDNIRRQEKMDIPTALSEFEYLTELKQIAGKNKIFKNFI"
BASE COUNT         1599 a          951 c          789 g         1204 t
ORIGIN      
        1 acaccttcag gcctcgcagc ttcttcaatt acctcaagct ctgctaccat gtcctgggca
       61 gcagtgtcag gagccaactc ctacgacctg cagtacaaga ccaattcatc aggcacctgg
      121 acaactgtca acacaacttc tacaagctcc acacttaccg ggcttacatc aggtactaca
      181 tacaacaccc aggtacgcgc tgtgtgcagc agtggcccaa gtgcatatag tgcacaggtt
      241 aattttacaa cagcctcagc aggttgtaca gataattacg agtccaataa ttcaaaaacc
      301 aatgcaaaaa ctatagccac aggtaccaat atcaatgcgc tcatctcaag ttcttcggat
      361 gttgattggt ttaagtttac cactacatct tcaaagaaaa acgtgaaagt gaatctttca
      421 aatctgcctg ctgactatga tatgaaattg tacagaagca gtacactcgt cgccacctca
      481 caaaactcag gtactcagga cgagcagtgc ataagcaaca acaccaagac agctactttc
      541 tacgtctata tctatggcta ttctggtgca tacgacccat cgaactgcta caatctgtat
      601 gttactgtca gcggatctaa tttcagaacg aatggagatg aagaatggac agaactaaca
      661 agagtaggtg acgagctact cgtctggcct aatcctgccc aaaacgaaat caatgtaaga
      721 ttgccattcg gagaagaaga agtatcaggt gatctaaaca tatttgatgc gacaggaaaa
      781 ttcatttacc agcaaaaact aaccggatcc aaagaaaatt ctgactaccg cattgatgtt
      841 tcttcattca gcaacgggct ttactttatc aactggaaga ctttatcaaa caatcttgtt
      901 caaaaagtga tcgtgacgaa ataacatttt aataattact gcataccaaa ggtcggcctg
      961 tcaagcagcc gacctttgaa tttttatcta taataaaatt tgaatgttaa tgtgggctta
     1021 ttgattgtat tcagtcatca aacccagata accacaatgg aaacaccagt aaactcagtc
     1081 aagcaaacac ctttaaactt tggtagtctg gttccatttt tttagctgaa taacaagcat
     1141 acttaaaccc aatccgcata ctattccgtt gccgaaataa tgtcctcggc tatcaatgtc
     1201 aagtgtaagg ttgaataaag acattgcgat gagcacaaca gaaagaaata aatagtgttt
     1261 tttcatttaa atagcatttg gttagaacga acctgcatca caaaactact cattttttta
     1321 caattttact cttttaaatc agaacttaac aagataactt gtataatatg tctggattct
     1381 gattgcagga ataaaagtat gattgtgaat acgaaaattt tacccaaaat cattcaataa
     1441 acaaaattag tcaaatcttt gtacgccgtt ataacattca ataaatgaac aaatctgtcc
     1501 gatattgtta ttagaatgaa aatgttcaac atcatgcaag tagtatgttc atcagattat
     1561 ctgtttagaa aaaattctga aggcaaggag cagtcaatat actatactat atatgcgcct
     1621 gccagtacaa ttgtacgcgc gacactactt gtacttcacg gaatgcagga acacagcggg
     1681 cgctacaaag aactgggaga atattttgcc caaaatggtt ttgctgtatt aacttacgat
     1741 cacctcggcc atggtaatac tgccaaaaat ctgagtgaac atgggtattt ccagatgcaa
     1801 aatcctgtag gccaattggt ggaggacaca agtttgatgg cagagaaact gcacacccaa
     1861 tacccgaacg ttccgcactt cattctcggc cattcaatgg ggtcgtttat tgcacgggca
     1921 ttcctacagc aacacagtga aacattcaca ggatcagtta ttgttggtac aggcggtaaa
     1981 attcccggaa tcgggatcgc caaatggtgc atggctatcc tcaacagact cattccacat
     2041 cgtcgaagta cattcgtcaa taatactttt agccgattca acagcattag gttcaggaaa
     2101 gatcccgatt acagccccat cagttggctt agcctcaacc ctttcaatag aaaaaatttt
     2161 gaagctgaca gactgaatgg catacctttc agttttaacg gatatcacac acttgtctcg
     2221 gtcaataaac gcgcaaccga acacgaatgg gcacacaaca tcagaaggga tttcccaatg
     2281 ttatacgtaa gcggatccga agacccaatt ggtaatttcg gcagaggagt attacagacc
     2341 actaatgagc tcataaggca aggattcagc aatatagctg taaaactcta cccaaaaatg
     2401 cgccacgaaa tactcaatga agagatcagg caagacgtat ttgcagaaat acttcaatgg
     2461 ttggaaacta agatgtaata taaaaagagt aaagagctat ctgctatgac aaagtccgct
     2521 acatattccg gaaacaattg tgatttgatt tcaaccaagc tgcagaaaat gtgtatctta
     2581 ccgaatgaaa tcagagataa tttgtaataa attggcaaat tacttaataa aaaaatacca
     2641 acaaaaaccc tggcaacaag ctgatgaatt caataaaaat aaactgcatt ctattcattt
     2701 tactgaccat gttgacacaa tcatcaattg ggcaaaatct ccttttgctt tcggataaaa
     2761 ccggaaaaaa tgacgtcata gtgcgtgtgg ataaaaaagt aaaggtaaaa accattaaca
     2821 aggaaaccat caaaggaaaa atcacctaca tcaaagatag taccatcatc atcggagaaa
     2881 aatcgcccag ggaaataaac atcggtgaca tcaaatattt ttatgttcgc aaggcagacg
     2941 gctggaaaac cgctactgcc accttgctgg cgttgactgc tgttgagtca acaattttcc
     3001 ttattcaggc gattgaaagc aaccagaaaa caccaccaaa ccaaggcacc atcgataatc
     3061 ctgtaaccgg ccttataggc ttgggtgcca tcaccattcc tgctacctat ctcggcacat
     3121 actatggatt ttttcacaag aagaaatatg acacgcggtt gagatatgca ttaaggatta
     3181 tcaaaccata ggcgttttct caacaatgaa agaatagtgg cgaatgtaat ttaacccaat
     3241 tagataataa aacccggcat tcacgatttg tctttgcaaa ttgcaccacc ttttatcaac
     3301 caggaaatac cgaaactttc gactgccaca gcttccatcc aaaatgttag atgatatctg
     3361 ttataggact cgacgggcat aatatatcct ttcgttccta aaaagatcaa aacaagtgca
     3421 agaatcataa tactaccaaa cactctatac atgcgatttc ggagtatttt ctcggatttc
     3481 cgttctccgg gatgtaaatc tgatttggtg aattgacaaa tggacatata ggccaagctt
     3541 cctaagaata aagctgcaaa aatgaaatgg gccacttgcc gataatcaga ttgtgggtgt
     3601 acgtaagtcc aaaccgtaac aatataatca tgactttctt ctaagtcctg aacaatattg
     3661 ccggtaggca acaacaataa caatattgct gctaagcccg caagacttga aacaaaaaaa
     3721 tcagtagaat cataaccttt gtaaatcatg aaaaatatag ccaaaacact gataatacca
     3781 ataaaaaatg ttgaagcctt ggtaaaaaaa taatgactaa tagaatccag cggctcacaa
     3841 tggcccgaaa aaaggtagag tgacagccag agaaataatg gtaatgccat gcccagaatg
     3901 ccaataacca aacgcaaagt tttgctactc tcaatgagta ttttgtcatc attcctgaat
     3961 gtaatgtcca gtgtgtcggt caatattttc aggtagcggt tttcatctgg aattttcctt
     4021 gccattttgg gtattattaa aatgtatggt gaaatgtatt ttacatatac aaatatatat
     4081 taatttaaaa tattatcaaa ctaatatttc atacaactta acaagtaaca aaacaattca
     4141 acaaccaaat tcagttataa tttatcacca cttaaatgat tcatcgtcac aattaactgt
     4201 accaatattc tctgtaacaa ttcgcaacaa acacattgcc atcacacata tttttactaa
     4261 atttgcagca ctaaaaaaat cagttagtat gaatacaata aaccctaaat tcctttctcc
     4321 tgacagattc atcaaccgac accttgggcc gagtgaagaa gaagctgccg aaatgctcaa
     4381 attaatcggt tatgatgatg taaacgcact gattcatgat accattcctg acaatatcag
     4441 acgacaggag aagatggata taccaacagc cttatctgag tttgaatatc tgacagaact
     4501 aaaacaaata gccggtaaaa acaaaatctt caaaaacttc ata
//
LOCUS       DAOQQY010000232         4542 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8875_length_4542_cov_5.147314, whole genome shotgun sequence.
ACCESSION   DAOQQY010000232 DAOQQY010000000
VERSION     DAOQQY010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8875_length_4542_cov_5.147314"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4260
                     /locus_tag="PLE23_10200"
     CDS             <1..4260
                     /locus_tag="PLE23_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83677.1"
                     /translation="NPAIGQGTLIGTVSIPSSDCDNASGVKFNEVMVKPANNDDDMSS
                     PTTGEWIELIGPPGTDISCYIISDGDWAITIPPGYSIPSDGLFVIGFTATAGPEVDLD
                     VAGCGCTTGNPNKVLTLDNNGEWLAMFDGTNTIDAFQYGSPSLLNTFPFGDLISGGTI
                     PGAMLIGCVQNYSPGFPSFTNYSPTTPTGQTYSRKPDFSGAWAVSCPTIGKCNTNGNS
                     FPLTLQASFSGFNCNQTIYLKALLTPTPGGCNTVISPTYSLFISCPESEFKKSLCAKE
                     TININGTIYDSSNPSGTEIIPGGSYLGCDSIIHVNLIFNPEVTATISGDFQICLGQSV
                     SIPVSFTGTPPFTFTYKLNGLPLNTVTTSSNPYILKFNPPTSGEISLSDLIDKANCEG
                     ETFGTVNVDVDAPTATLSGKKYKVCEGDTVYIPVNLFGYPDFKLSYALNGKLQNEITT
                     SQLLYNIPVVVNANTIITIKEMTDDQGCQAKISGQDTIIVTPKIKIKDLTETCYPGNT
                     FDVTIILSGGDSATWKVIGPGTLKDSIFTSSSFTGGSTYKFIIKDTTGCSIDTISNTV
                     KCNCLNAVGTMDQTTLNACVDGTVNATYNTAGQNLGPGDILIYILHENPGNSPGKIWA
                     QNATPDFGFVAGMTPGIVYYISAVIGKENPPGQIDLSDECLQTAKGTPVIFHDFPSVV
                     LSPDTSVCVGNCVDISSVISGNEPFDISYDITSNLGTNTFNSANQASNISYTYCPPTS
                     NGQGTTTFTITTIKDKYCTATFTESIKISVGTPTTSNLNKDLCYGQTIIVKGNVYSET
                     NPTGTELLPAANQFGCDSTVFVSLSFVNTVIENLNKTICEGSSITINGTIFDKNNPTG
                     SFTFPGGSVSGCDSVLNVNLFFYPQSKGKLEQTLCAGESLIINGKIYDVANPSGFENL
                     AGKALNGCDSLLEVNLIFLPPVFKLYRDTLCKGESVVVNGTKYDINKPAGLEIFKNAS
                     AAGCDSTVQVQLVFVDELTATLTGPDTICTGQEPEITIHFSQPGSYDVVISDGKGQNF
                     SFTGITDGFTLKPGILTTSTSIGFASVVALTGNCSVTLGQSLDIFVGSLPADILAMSD
                     YNGYNISCNGLADGSVQAITTGGTAPFSYLWSGGQTTNIITGVLAGTYTVTITDAGAC
                     SVEKSITLTEPQTLSAEFKANPVACNGTSGGSITIIQLSGGTGSYTYRLNGTNQPLPV
                     KDGDVIPNLAEGTYLLSIEDKNGCSINDNIIIEPTSSAGLFVDAGADVTIEANKQWGL
                     EVETNLVPFEINWNPARGLDCINCLNPVVKTDSTTTYTVVITDKNGCTATDSITVFIL
                     SPDIVYIPNVFSPDGDGINDRVTVYAGNRVKAIRIFEIFDRWGEKVFSRTDMPANEPE
                     AGWDGKHRGQKVQEGVYVYYLLIELTDGKKRLLKGDISLIQ"
BASE COUNT         1308 a         1097 c          964 g         1173 t
ORIGIN      
        1 aaccctgcca ttggacaagg caccctgata ggcacagtaa gtattccttc atctgactgt
       61 gacaacgcaa gtggtgtcaa atttaatgaa gtaatggtaa aaccagccaa taatgatgac
      121 gacatgagct cacctaccac aggagaatgg attgaactca tcgggcctcc gggcactgat
      181 atcagctgtt atattatctc tgacggtgac tgggccatta ccattccacc cggctattct
      241 ataccatccg acggactatt tgttatcggt tttacagcta ctgcaggccc tgaggtggac
      301 ctcgatgtgg caggctgcgg gtgcaccaca ggcaacccga acaaggtttt gaccctcgac
      361 aacaatggtg agtggctggc tatgtttgac ggcaccaata ccattgatgc ttttcagtat
      421 gggtcaccaa gcctgctcaa tacctttccg ttcggcgatc tgatatcggg cggaaccatt
      481 ccgggcgcta tgcttatagg ttgtgtacaa aactattcgc ccgggtttcc ttcatttacc
      541 aattattcac caactacacc taccggacag acttattcgc gcaagccgga tttctcagga
      601 gcttgggcag tgagttgtcc taccattggc aaatgtaata caaacggtaa cagcttcccg
      661 cttacactac aggccagctt ttctgggttc aactgcaatc agactattta tctgaaagct
      721 ctgcttacac ctacaccggg cgggtgcaat acggttatct ctcctactta ttctcttttc
      781 atttcatgcc ctgagtcgga gttcaaaaaa tcgctttgcg ccaaagaaac cataaatatc
      841 aatggcacta tctacgacag cagcaaccca agcggtaccg aaataatacc gggaggaagc
      901 tacctgggct gtgacagtat tattcatgtc aatctcattt tcaatcctga agtaacggcc
      961 accatcagcg gcgattttca gatatgcctc gggcaatcgg tttccatacc ggtaagtttt
     1021 acgggcacac ctccctttac atttacctac aaactcaacg gtctgccact caataccgtc
     1081 actacctctt ccaaccctta tattttgaaa ttcaatcctc ccacgtcggg tgaaataagc
     1141 ctttctgacc tgattgacaa ggcaaattgt gaaggagaaa catttggcac agtcaatgta
     1201 gatgtagatg cccctaccgc cactctgtcg ggcaaaaaat acaaggtatg tgaaggtgac
     1261 acggtttata ttccggtgaa tttgtttggt tatcctgatt tcaagctcag ctatgcactt
     1321 aatggaaaat tgcaaaacga aattaccacg agccaactcc tttataacat tccggtagtg
     1381 gtcaatgcca atacgattat taccatcaag gaaatgacgg acgatcaggg ttgtcaggca
     1441 aaaatttcag gacaggatac catcattgtc acaccaaaaa ttaaaatcaa agacctgacc
     1501 gagacctgct acccgggcaa cacttttgac gttaccatca ttctttcagg aggtgattcg
     1561 gctacatgga aggtcatcgg gcctggcaca ctcaaggact cgatttttac cagcagcagc
     1621 ttcaccggtg gctctacata caaattcatt ataaaagata ctacaggctg cagtattgat
     1681 acaataagca ataccgtcaa gtgcaattgc ctgaacgctg taggcacaat ggaccaaact
     1741 accttgaatg cctgtgttga tggaacagtt aatgccacct acaacacagc cggacaaaat
     1801 ctcgggccgg gcgatatact gatatacata ctgcatgaaa atccgggtaa ctctcccgga
     1861 aaaatatggg cacaaaatgc tactcccgat ttcggcttcg tagccggtat gacacccggt
     1921 atagtttact acatctcggc agtcatcggc aaggaaaatc ctccgggaca gatagacctg
     1981 tcggacgaat gcctacagac agctaagggc acaccggtta tctttcacga tttcccttct
     2041 gtagtgctct cgcctgatac cagcgtatgt gtcggtaact gtgttgatat atcatctgtc
     2101 atcagcggaa atgaaccatt tgacataagc tatgacatta cttccaacct cggaaccaat
     2161 acattcaatt ctgccaatca ggcttcaaat attagctaca cgtactgccc acccacgagc
     2221 aacggtcaag gcacaaccac tttcactatt accactatca aggacaaata ctgcactgcc
     2281 acctttaccg aatcaataaa aatatccgta ggtacaccca ctacatctaa cctcaacaag
     2341 gacctgtgtt atggccagac catcatcgtc aaaggaaatg tgtattctga aaccaatccg
     2401 acaggaacag aattattacc ggcggctaac cagttcggct gcgacagtac agtttttgtc
     2461 agcctaagtt tcgtaaatac tgtcattgaa aatctcaaca aaacaatctg tgaaggctcg
     2521 agcataacca tcaatggtac catttttgac aaaaacaatc ctacaggcag ttttactttt
     2581 ccgggtggta gcgtttcggg atgtgattcg gtacttaatg tgaacttgtt tttctaccca
     2641 caaagcaagg gcaaactcga acaaactctc tgcgccggtg aaagccttat cataaacgga
     2701 aaaatatacg atgtcgccaa tccttcggga tttgaaaatc tggcaggaaa ggcattgaac
     2761 ggctgcgatt cattattgga ggtcaatctg atctttctgc cgcctgtatt caaactctac
     2821 cgcgacaccc tttgtaaggg tgaaagcgtt gtggtgaacg gcaccaaata tgatataaac
     2881 aaaccagccg gtctcgaaat attcaagaat gcaagtgctg caggctgcga tagcacggtt
     2941 caggtgcagc tcgtgtttgt tgatgaacta acagctacac ttacaggccc tgacaccatc
     3001 tgtaccgggc aggagcctga gataacaatt catttcagtc aaccgggcag ctatgatgtg
     3061 gtcatcagtg atggtaaagg tcaaaatttt agttttaccg gcatcacaga cggatttacc
     3121 cttaaacctg gcatcttgac gacttctacc agtattgggt ttgcttctgt tgttgccctg
     3181 acgggaaatt gcagcgtcac cctcggacag tctcttgaca tatttgtcgg ttcacttcct
     3241 gccgacatac tggctatgag cgactacaat ggctacaaca tcagttgcaa tggcttggct
     3301 gatggctctg tacaggctat tacaacgggt ggtacggcac cattcagcta tctttggtca
     3361 ggaggccaga ctacgaacat tattaccggt gtacttgccg gaacttatac cgtcaccatt
     3421 acggatgccg gtgcttgttc tgttgaaaag tcgattacac ttacggaacc acagacatta
     3481 tcagcagaat ttaaagccaa cccggtggca tgcaacggca cttcaggagg cagcattaca
     3541 attattcaac tatccggcgg cacaggtagt tacacttatc gtttgaatgg aacaaaccag
     3601 ccattgcctg taaaggacgg agatgtgata ccgaatctgg cagaaggaac atacctgtta
     3661 agcatcgagg ataaaaatgg ctgcagcata aatgacaata tcataatcga acctaccagt
     3721 tcggcaggat tgttcgtcga tgccggagca gatgtaacga ttgaagccaa taaacagtgg
     3781 gggcttgaag tagaaacgaa cctcgtccct tttgaaataa attggaatcc ggcccgggga
     3841 cttgactgta tcaattgtct caatccggta gttaaaacgg attctaccac tacttatacg
     3901 gttgtcatta ccgacaaaaa cggctgcaca gccaccgaca gcatcacagt tttcatatta
     3961 tcgcccgaca ttgtctatat cccgaatgta ttcagccctg acggcgacgg catcaacgac
     4021 agagttactg tttacgcagg aaacagggtc aaggctatcc ggatatttga aatttttgac
     4081 cgctggggcg aaaaggtgtt cagccgaacc gacatgcctg ccaacgaacc cgaggcagga
     4141 tgggacggaa aacacagagg acaaaaagtc caggaaggtg tatatgttta ttacctgcta
     4201 atagaactaa ccgacggcaa gaaacgttta ctgaaaggtg atataagtct tattcagtaa
     4261 tttttgaaaa atatatcgaa aacaactatc agtacaaata gtatgtatct tgtattttca
     4321 tcttagctga ttttatggat ctttacaaat ttattctctc cacccggcac gattatcctg
     4381 aagattgatt tcagcataaa aaacgaagcc ctccacaggc tgcttttcgc cggtatgttt
     4441 gccagcctta tttatacttt acatccttaa cctttcccca tgtcatgtct ttgcaataat
     4501 caggaagagg ggtaagaaac atagtgtcta acttgggaat at
//
LOCUS       DAOQQY010000233         4533 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8894_length_4533_cov_4.383207, whole genome shotgun sequence.
ACCESSION   DAOQQY010000233 DAOQQY010000000
VERSION     DAOQQY010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8894_length_4533_cov_4.383207"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>4533)
                     /locus_tag="PLE23_10205"
     CDS             complement(<1..>4533)
                     /locus_tag="PLE23_10205"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMX83678.1"
                     /translation="YGEVYYGNIERSTNGGVSWASIKPSGSGNGQWITPFTLDPTTPT
                     TIYMGFAQIYKSTNQGNSWSTISPNFGLGAMSMINVAPSNSNYIYTGTSGALRRTTDG
                     GATWSNVTVPGSGTVMLAIHPLDPNTLYAVRSNYTAGAKVYKSTDGAATWTNISGSLP
                     NLPANCIVYANCTQDGLYVGMDAGVYYKDNTMADWILHNDNLPNAEIRELEIDYNEKK
                     IYAATYGRGMWKADLFMSAPLITITAGTTTGPTTCGGSDGSIVLNFTNVPNGSYLISY
                     KRNGTSASQTVTVTSNSASLTGLNAGNYTEFSITAVGCVGTLAGPVVLNDPALPVVDP
                     VASQSVCAGAPVAAVNFSGTAGATFNWTNDNVNIGLAASGSGNITGYTAPGVLTTQTG
                     NIVVTPVKAGCVGNPTSFTITIKALPVISVNSQVDPTCGNFDGSISFGFTNVPDGNYI
                     LSYTRNSIPQTSTITVLGGLATLTGLGDGMYAGFSITANSCTGTSTTTVTLSCGTAPT
                     CPSIGTLNSGVSVSCKSTNFTLTANGLANMGSTYGISFISSTTPLADPYTGGTLLGTV
                     PNSGLLGGGTQAVLNTSINTAASYYIYAIISPLPSDPGCRPSKNNTIKILDCTPVITS
                     PCSCKNNATTLTNGQFDETITVNAPAGQTWTVTAVSGLYQTSSPAPPGTPVVIPIGYT
                     LTPGAVLGDGSTNYTLLGIHVDAVGYSVTVSNGTANGSVGATCYYPNPQINALAPAYC
                     QNEPSVTLSGSATLGGPPGGAAIGTGTFTVNGTPATVFDPSVVGAGTHTVVYTFDAAD
                     GVPNASHPGCIQSVSQSVVVNPVPTANAVASATYCPGASVPASNFSGTPSGVTFSWTR
                     TAGAIGLAPLSGNGNVPSFVATNGGATPISSTFTVTPSYTNAGKTCTGTPVNYTITIN
                     PTPTVNAQTNKTYCSSTSTGGITFTGNATKYNWTNTNTAIGLASSGTATPSIPSWVPV
                     NNGGAPVTGTITVTPVYTNNGVDCFGSPITFTITIIPTPNVNAQTNKTYCSGTPTGGI
                     TFTGTATAYNWTNTNTAIGLAANGTASPTIPSWIPVNNTNFPISGTITVTPEYTLNGV
                     TCSGAPISFTITVNPGAKAVCKDFTLYLNNTGTATLTPANIDGGSTGGTLSISKTGFN
                     CADVGNNAVILTVTDICNGTSTCAAIVKVVDNIAPVINGVPADLTIECDGTIPAVPTV
                     TATDNCSAILTFAETNNKTSHTTLCSSISYDIKRIWTAVDPSGNKTVKTQVIKLVDTK
                     APTFTLVPPAFLTVECDDDADNNIDPIAVDGCDSQPTMLLDIKYNFNPNGCKNSYVAT
                     YLWTAGDKCGNTAQYIQQITVEDQEPPVIVCPPNIVLVSQTPVSASWILPKSYDYCDG
                     ILQVKQIAGPPSGSLFTPGTITTIVYQATDLCGNVSTCSFTVGVKFGGSKIGFDVSGK
                     VLSALNPAKNIHAEAVLSGGMNQFTETNNGSYLFSAIPEGTNLTVSAKQEKYPLNGVN
                     ALDLVYITNHVLGKKPL"
BASE COUNT         1234 a          991 c         1044 g         1264 t
ORIGIN      
        1 agtggttttt taccaagtac gtgattggtt atatacacaa ggtctaatgc attcactccg
       61 ttgagcggat atttttcctg ctttgctgaa acagtcaaat tggtaccctc cggtattgca
      121 ctaaacagat agcttccgtt attagtttca gtaaattggt tcattccacc gctgagtact
      181 gcttctgcat gaatgttctt tgcaggattc aatgcagaca agacctttcc gcttacgtcg
      241 aaaccaatct tcgagcctcc aaattttacg ccaactgtaa acgaacaggt actcacattg
      301 ccacacaagt cagttgcctg atagacgatg gtggtgatag ttccgggtgt aaataaactt
      361 ccgctcggtg gacccgctat ttgtttcacc tgaagaatac catcacagta atcataagat
      421 tttggtaaaa tccagctcgc acttacaggt gtctgactga cgagcactat atttggcgga
      481 catacaatga caggtggctc ctggtcttct actgttatct gctggatgta ttgggctgta
      541 ttgccacatt tgtcgccggc agtccatagg taggtggcta catacgaatt tttgcagcca
      601 ttcgggttga agttatattt gatgtcaagc agcatcgtag gttgcgagtc gcacccgtca
      661 acagcaatcg ggtcaatatt attgtctgca tcgtcgtcac attcaactgt aaggaaagcc
      721 ggtggaacca atgtaaatgt tggtgccttt gtatcaacca acttaatcac ctgtgtcttc
      781 acagtcttat tgcccgaagg gtcaacagcc gtccatatcc tcttgatgtc ataactaatg
      841 cttgagcaaa gggtagtatg gcttgtttta ttgttagttt ctgcgaatgt caatattgca
      901 gaacaattat cagtagcggt cactgtcggc acagcaggaa tagttccatc gcattcgatg
      961 gtaaggtcag caggcacacc gttgatgaca ggtgctatgt tgtccacaac ctttactata
     1021 gcagcacaag tagaagtgcc attacaaata tctgtaacag tcaggataac ggcattatta
     1081 cctacatcag cacaattgaa accagtcttg ctgatgctga gtgttcctcc tgtgctacct
     1141 ccgtctatat tggccggtgt gagagttgct gttcctgtat tattaagata taaggtaaag
     1201 tccttacata ccgccttagc accaggatta actgttattg tgaaagatat cggtgcccct
     1261 gaacacgtaa ctccgttaag tgtatattcg ggcgtaaccg tgatggttcc tgaaatcggg
     1321 aaattggtat tgttaaccgg aatccatgat ggaatggtcg gtgaagctgt accattagca
     1381 gccaggccta ttgcagtatt ggtattagtc cagttgtaag cagtagctgt acctgtaaag
     1441 gttattccac cggtaggtgt acccgaacag tatgttttat tagtctgggc attaacattt
     1501 ggtgtaggta taatggtgat tgtaaaagta atcggcgaac caaaacaatc aaccccattg
     1561 ttggtataaa ccggtgtaac agttatcgtt ccggttacag gtgccccacc attattgaca
     1621 ggcacccatg aagggatgga aggtgtagct gtaccgcttg atgccagacc gatagctgta
     1681 ttggtattag tccagttgta tttagtagca tttccggtaa atgtaatacc acctgttgat
     1741 gtgcttgagc aatatgtctt attggtttga gcattgacag ttggtgtcgg attgatggta
     1801 atggtatagt tcacaggtgt acctgtacat gttttaccgg cattggtata gcttggggta
     1861 acagtaaatg tgcttgatat aggtgttgca ccaccatttg tggctacgaa agaaggtaca
     1921 ttgccattac cactcaaagg ggcaagacca atagcacctg cagtcctagt ccagctgaat
     1981 gtaacacctg atggtgtgcc actgaaatta gatgccggaa cagaagcacc cggacaatat
     2041 gtagcactgg caacagcatt tgccgttggc acaggattga ctactacact ttggctcact
     2101 gactgaatac aacccggatg gcttgcgtta ggcactccgt ctgctgcatc gaaagtatat
     2161 acaacagtat gtgtacctgc acctacgact gacgggtcaa acacagtagc aggcgttccg
     2221 tttacagtaa atgttccagt tcctatagca gcaccacccg gagggccacc taaagttgct
     2281 gagccactta gtgtcactga cggttcattt tgacaatatg caggagcaag tgcattaatc
     2341 tgaggattcg gataataaca cgtagcacca accgatccat tcgcggtacc gttcgacact
     2401 gtcacactat aacctacagc atctacgtgg atacccaaca aagtataatt ggttgatcca
     2461 tcacccaata ctgcaccagg agtgagtgta taacctatcg gaatgacgac cggtgtaccc
     2521 ggaggtgccg gagaagatgt ttggtacaga cctgaaaccg cagttactgt ccatgtctga
     2581 ccggcaggcg cattgaccgt aatagtttcg tcaaattgac cattggtaag ggtagtagca
     2641 ttattcttgc atgaacacgg acttgtaatt accggagtac aatccagtat tttgattgta
     2701 ttattcttag aaggacgaca acccgggtca gaaggcaatg gactgattat agcataaata
     2761 taataacttg ctgctgtgtt gatactggtg ttcaatactg cctgcgtgcc tccaccgaga
     2821 agcccgctgt taggaactgt acccagcaag gtacctccag tgtaaggatc agcaagcgga
     2881 gtagttgagg aaataaaact tatgccgtat gttgagccca tgttggcaag tccattcgct
     2941 gttaaggtga aatttgtaga tttacatgaa acagaaacac ctgaattaag tgtaccaata
     3001 gaaggacaag taggagcagt gccacatgaa agcgtgacgg tagtagtaga tgtgcccgta
     3061 caactattag cagttatgct gaatcctgca tacataccat cacccaaacc tgttagggtt
     3121 gccaagccac caagtacagt aatggttgat gtttggggaa tactattgcg tgtataagag
     3181 agtatatagt tgccatccgg cacattggta aacccgaatg aaatacttcc gtcaaagtta
     3241 ccgcatgtag ggtcaacctg tgaattgacc gaaataaccg gcaaagcttt gatggtgata
     3301 gtaaatgatg ttggattacc gacacagcct gcttttacag gcgtaactac aatatttcct
     3361 gtttgggtag taagcacacc tggtgctgtg tagccagtga tattgcctga gccacttgct
     3421 gcaagaccga tattgacatt atcgtttgtc cagttaaatg ttgctcctgc tgttccggaa
     3481 aaattaacgg ctgcaaccgg tgcacctgca catacgcttt ggctcgcaac agggtcaaca
     3541 accggcaatg ccggatcatt caacacaaca ggccctgcca atgtgccaac acagcctact
     3601 gctgtgatac taaattccgt ataattcccg gcatttagcc cggtcaagct tgccgaattg
     3661 gatgtaaccg ttactgtttg agaggctgaa gtaccatttc ttttataact aattaggtat
     3721 gaaccatttg gaacgtttgt aaaattgaga acaatacttc catccgaccc accgcaagta
     3781 gtaggcccgg tagtagtacc ggcagtaatg gtaatcaaag gtgcagacat gaacaagtcg
     3841 gctttccaca ttccacgacc ataggtagca gcgtagattt ttttctcatt atagtctatt
     3901 tcaagttccc tgatttcagc attaggaaga ttgtcattgt gcaatatcca gtcggccatt
     3961 gtattgtctt tgtaatacac acctgcatcc atacctacat aaaggccatc ctgtgtacag
     4021 ttggcatata cgatacagtt cgcaggcaga ttcggtaatg aaccgcttat atttgtccag
     4081 gtggcagcgc catcggttga tttatatacc ttggcccctg ccgtataatt agatcttaca
     4141 gcgtagagtg tatttgggtc aagcggatgg atggccagca tgaccgtacc actacccggc
     4201 accgtaacat ttgaccaggt agcaccgccg tctgttgtcc ttcgcaacgc accgctggtg
     4261 ccggtataaa tatagtttga gttagaagga gcaacattaa tcatagacat tgcgcctaag
     4321 ccaaagtttg gtgaaatggt actccatgaa tttccctgat tggttgattt atatatttgg
     4381 gcaaaaccca tataaatagt ggtaggtgta gtagggtcga gggtaaaagg cgtaatccac
     4441 tggccgttac cggagccact aggcttgata cttgcccaac ttacaccacc gttggtcgat
     4501 ctttcaatat tgccataata aacttcacca tac
//
LOCUS       DAOQQY010000234         4516 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_8931_length_4516_cov_7.072629, whole genome shotgun sequence.
ACCESSION   DAOQQY010000234 DAOQQY010000000
VERSION     DAOQQY010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8931_length_4516_cov_7.072629"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2227
                     /locus_tag="PLE23_10210"
     CDS             <1..2227
                     /locus_tag="PLE23_10210"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HMX83679.1"
                     /translation="NNLAATHGYFSEAQLEAYLNNSGVVSKLQFIGKEEQIGSFVHQN
                     GNTGMTSGLIISSGDCELATGPNTSESMGTTMQGGDDTDLKQIAVNTMLYDAGGFEFD
                     LLATSDYLTCWLVFASEEYNEYTGSSFNDLFGVFVSENGSSGTNIATIPGSKLPIRIN
                     NVNQGAPGMNGDIQNVTPPLGSTAYSKYYTSNPTYLPNDTIYYGIEYDGFTKPFLIKY
                     PMETGKSYHFKFVIADAGDGVYDSGLLITNDEYQVNGIAEPIIAYTQSVCGNTVSVQN
                     HTKYGVDYLWDFGDGTTSTQAEPPPHTYAVQGNYQVKLKAKSSANLVAEMQKPVIFNP
                     EAELVNTQISHIDCNSSGKIKLEINSVASANMTYQWSDDNTVKNAQNINCSFIIEKEV
                     PEAGSYTVTVTSNNHAYTFGPYNVVYSGNKLDLATNVKPACTKQANGEIELVFTNGNP
                     TDINWSHDASLKSYIATGLPAGAYTINYTDNHGCIKTVNIEIGNKELPDQNNISVTPP
                     DCNFDKGLIYVAVPESNGIASGTLLNSQNQVVATQNSALTFIFSNIASGNYTLLYSDE
                     EGCTTTLNLYMPDKQIPQSKLLTSTITDNNKTVSAILTQQGKAPFTYTWSDGFNGSVR
                     SDLSAGKTYKVTVTDANGCTEILNLVIKPNSSDVVSGFTAFPVPADQYVMLRQCNPEQ
                     IDALTEIQVLDSSGKLYRIEKIREGEEIRLNTASLPNGLYFVHINSSNGEHILVLSVL
                     H"
     gene            complement(2291..2746)
                     /gene="bcp"
                     /locus_tag="PLE23_10215"
     CDS             complement(2291..2746)
                     /gene="bcp"
                     /locus_tag="PLE23_10215"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HMX83680.1"
                     /translation="MTHLKPGDAAPDFSGVNENNEPISLKDFAGKKLVLYFYPKDDTP
                     GCTAEACSLRDHYEKFLSEGYAILGASPDSPKRHTAFIKKYNLPFSLLSDESKQTLLD
                     YGVWGPKKFMGRSYEGVYRTTFIINENGIIDEVITEVNTKNHASQILAG"
     gene            2887..3366
                     /locus_tag="PLE23_10220"
     CDS             2887..3366
                     /locus_tag="PLE23_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83681.1"
                     /translation="MKKITTIFLSLFLLMNTSSASQLTKCNNVTRENFTQEAPFGSLI
                     TTLVNGLKPTAFSKDWKTQSASFLQKMKTQDAKDLTGSASALFELYKLLNPKTMSKEW
                     KTEKGAFLHGVSEVKSKEDLSRLLLLLVNNISPTYFTKEFKAQLESFKKTIGSLGMN"
     gene            3719..4231
                     /locus_tag="PLE23_10225"
     CDS             3719..4231
                     /locus_tag="PLE23_10225"
                     /inference="COORDINATES: protein motif:HMM:NF019614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HMX83682.1"
                     /translation="MMYAVLLGGVNIGGRTVKSDELKQSFIKAGFKNPQTVLASGNVI
                     ISSDKNTDDLKNEIEAFLLKDFNFSIQVIVVDFVTLQKIVEAYPFQEAGPEYHRYIIF
                     REKDTLLDTKMVQAETEAIVQYKNIIYWCVLKGMTLESVFSKYQAKLARKEFSTTRNI
                     NTIEKILAKA"
BASE COUNT         1450 a          915 c          892 g         1259 t
ORIGIN      
        1 caataatttg gctgccaccc acgggtattt ttcagaggcc caactagaag cttatctcaa
       61 taattcaggc gtggtaagca aacttcagtt cattggtaaa gaggagcaaa tcggttcatt
      121 cgtgcatcaa aacggtaata ccggtatgac aagtggtctg attatatctt ccggagattg
      181 cgaactggcc actggtccta atacatcaga atctatgggt acaacaatgc aaggaggcga
      241 tgataccgat ttaaagcaaa ttgcggttaa tactatgctt tatgatgccg gcggatttga
      301 gtttgattta ttggctactt ccgattacct tacatgctgg ctggtttttg cttctgaaga
      361 atataacgaa tataccggaa gttcattcaa cgatttattt ggtgtattcg taagtgaaaa
      421 tggctcttca ggtaccaata tagctaccat accgggtagt aagcttccta tcagaataaa
      481 taatgtaaac cagggcgccc caggtatgaa tggcgatatt caaaacgtaa cacctccgct
      541 tggttcaacc gcatattcca aatattatac cagcaatccg acctacctgc caaatgatac
      601 catttattat ggcatagaat atgacggctt tacaaagcct ttcctcatta aatatcctat
      661 ggagacaggt aaaagctacc actttaagtt tgtcatagct gacgcaggag atggcgtgta
      721 tgacagcgga ttgctcataa ccaatgatga gtatcaggta aatggtattg ccgaaccaat
      781 cattgcatat acacagtccg tctgtggcaa cacggtatcg gttcaaaacc atacaaagta
      841 tggcgttgat tatctatggg atttcggaga tggtactacc tctacccaag ctgaaccacc
      901 accacacact tatgctgtac agggtaatta tcaggtcaaa ctgaaagcta aaagttcagc
      961 caatttagtg gcagaaatgc agaaaccggt catttttaac ccggaagccg aattagttaa
     1021 tacccaaatc agccacatcg attgcaactc ttcaggcaag ataaagcttg aaatcaactc
     1081 tgttgcttcg gctaatatga cgtatcagtg gtcagatgat aatactgtaa agaatgcaca
     1141 aaacattaac tgcagcttta tcatcgaaaa agaagtgcct gaagccggtt catatacggt
     1201 gaccgtcact tcaaacaatc atgcttacac attcgggcca tacaacgtcg tttattcagg
     1261 taacaagtta gatctggcta caaatgtaaa acctgcctgt accaagcaag caaacggtga
     1321 aatagaactg gtcttcacca acggaaaccc tactgatatt aattggtcgc atgatgcctc
     1381 attgaaaagc tatatcgcaa ccggcctacc tgcaggtgct tataccataa actatacaga
     1441 caaccacgga tgtataaaaa ctgttaatat tgaaattggc aataaagaac tacccgacca
     1501 gaataacatc tccgttactc cgcctgattg caactttgat aaaggactca tttatgtggc
     1561 ggttcctgaa agtaatggta tagctagcgg aacattgctc aacagccaga atcaggtggt
     1621 agcaacacaa aattctgcat taacattcat tttttccaat atagcttcag gcaattatac
     1681 actgctttac agtgacgaag aaggctgtac caccacactc aatctatata tgccggataa
     1741 gcagattcct caatctaaac tgctcacttc taccattaca gataacaaca agaccgtatc
     1801 agccatcctt acccagcaag gaaaagcccc gttcacttat acctggtctg acggattcaa
     1861 tggctcggta cgctccgatt tgagtgccgg caaaacctat aaagtaaccg tcacggatgc
     1921 caacggatgt acagagatat tgaaccttgt tatcaaacca aattcatcag atgtagtcag
     1981 tggctttacg gcttttccgg ttccggcaga ccaatatgta atgctacgac agtgtaaccc
     2041 cgaacagata gatgccctaa cagaaataca ggttcttgac agctccggta aattatacag
     2101 aatagaaaaa ataagggaag gtgaagagat acgactcaat actgcaagtt tgcctaacgg
     2161 tctgtatttc gtacatatca acagtagcaa tggcgaacat atattggtat tgtctgtact
     2221 gcattaaagc aatacacata tttaccctta ttgcattggc aaataaacct tactgagtat
     2281 caaatatcta tcagccagcc agtatttgtg aggcgtggtt cttggtgttt acttccgtta
     2341 tgacctcgtc tatgatgcca ttttcattga tgatgaatgt agtgcgataa acgccttcgt
     2401 aagagcgacc catgaacttc ttcggccccc agactccata atcgaggaga gtttgcttac
     2461 tttcgtctga aagcagactg aatggcagat tatacttttt gatgaaagcg gtgtgccttt
     2521 taggcgaatc ggggctggca ccaagtatgg cataaccttc ggagagaaat ttttcgtagt
     2581 gatcgcgcag actacatgct tcggctgtac aaccgggcgt atcatctttt ggataaaaat
     2641 aaagtacgag cttctttcct gcaaaatcct taagtgaaat gggttcgttg ttttcgttga
     2701 cacctgaaaa atctggtgcg gcatcgcctg gttttaaatg ggtcatggca gctgttttat
     2761 tcattctgaa caacaggatt tgtgaaaagt tctaattttt ttccggttac agctagaaga
     2821 tattagataa tcatcagcaa aatgcttaac tttggcatca tccgtcacta attaaatatt
     2881 tgacacatga aaaagataac caccattttt ctcagccttt ttttactgat gaatacttca
     2941 tccgccagtc aattaaccaa atgtaataat gttacacgag aaaatttcac gcaggaagca
     3001 cccttcggta gcctgataac cacattggtg aatggattaa aacctacagc cttctcgaaa
     3061 gactggaaaa cgcaaagtgc ttcgtttctt caaaaaatga agacacaaga cgccaaggac
     3121 cttacaggct cagcctctgc cctattcgaa ttgtacaaac tactcaatcc gaaaaccatg
     3181 tctaaagaat ggaagactga aaaaggtgcg tttcttcatg gtgtatctga agtgaaaagc
     3241 aaggaagatc tcagcagatt acttcttctg ttggtgaaca acatttcgcc tacttatttt
     3301 accaaagaat ttaaggccca gctcgaaagt ttcaagaaaa caattggtag cttgggtatg
     3361 aattaaacct ttataacaca cttgtggctt gtgctatatt ctgagccaaa agaatgaaag
     3421 gaaatcagct acagcttggg taactaatat ggctaccaac aagtctgact ctctaaatct
     3481 ctccaagaaa tgcgctgtaa ttaccgacac gatgagattt cgaaaaccaa caggtgtctg
     3541 ttgaccatca aatactgtgt agcgaaaggt ggaagtggtt gtgcgtgtat aattggaaga
     3601 ttaaagctga gaaaaactgt taatatgtac cgattataat attttaatgg tgaaaaattt
     3661 gtttacaaac ccatttgtat ccgactattg gtatctttat aattgaatga cgaatattat
     3721 gatgtacgca gtattgttgg gcggtgtcaa tattggtggc agaacggtta agtcggatga
     3781 gttgaagcaa tcttttatca aggccggatt taaaaacccg caaaccgtac tggcaagcgg
     3841 aaatgtcatc atatctagtg acaagaatac cgacgacttg aaaaatgaaa tagaagcatt
     3901 cttgttgaaa gattttaatt tctccattca ggtcattgtg gttgatttcg tcactttaca
     3961 aaaaattgtg gaagcctatc cgtttcagga agccggcccc gagtatcatc gttatattat
     4021 tttcagagaa aaagatacat tgcttgacac aaagatggtt caagcagaaa cagaagctat
     4081 tgtacagtat aaaaacatca tttactggtg cgtattgaag ggaatgacac tagaaagcgt
     4141 attttcaaaa taccaggcca aactagcccg aaaggaattt tccacaacaa ggaatatcaa
     4201 taccattgaa aaaattctgg caaaggcata atggtttaaa acttgaaatg aagccatctg
     4261 aaagatgtag aattaccgga tgaatactta attttaccct tcggaatcag aacatattcg
     4321 gtaaagtgta tttgaaaaaa ccaacagttt ccggccaatt tgccctaaat taaataattt
     4381 ttttaaccaa cattaaatta aacccaaaat ggtagcagta aatgtgtatc ttactttcga
     4441 tggcaactgt gaggaagcct tcaattttta caaatctgtt ttcggtggtg aatttgccgt
     4501 attcacccgg ttcaac
//
LOCUS       DAOQQY010000235         4461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9097_length_4461_cov_5.884703, whole genome shotgun sequence.
ACCESSION   DAOQQY010000235 DAOQQY010000000
VERSION     DAOQQY010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9097_length_4461_cov_5.884703"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /gene="mnmE"
                     /locus_tag="PLE23_10230"
     CDS             <1..1129
                     /gene="mnmE"
                     /locus_tag="PLE23_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMX83683.1"
                     /translation="SCHGSPYIQQQIIGLFLADGVRLATPGEYTMRAFLNGRMDLSQA
                     EAVADLIAAESATGHQIAMRQMRGGFSDELSGLREELVNFASLIELELDFGEEDVEFA
                     NRSQLVELLEKIIHTIDGMIYSFELGNAIKNGVNTVIAGRPNAGKSTLLNALLNEERA
                     IVSEIAGTTRDTIEEVLILEGIRFRLIDTAGIREAQDQIEAIGVRKTFEQASKASILI
                     YVFDVTECSPEQVHEDLEKLNHPNEKTLVIANKMDLNPYAKPENYAIGSVSVDNIITA
                     SAIHKMNIPYLMSRITSLVKSGEVTAGSTIISNSRHLESLTAARESLHKAKENIQAGV
                     TGDFIAMDIRNALYHIGSITGKIDVDQDILGTIFGRFCIGK"
     gene            1348..1749
                     /locus_tag="PLE23_10235"
     CDS             1348..1749
                     /locus_tag="PLE23_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HMX83684.1"
                     /translation="MSSNIKIPKFCQYCGQAFVAQKTTTRFCSHKCASRAYKQRKREE
                     KVGSTLKEQIKSVTSANSENLQSLQSLPIKTGNFINLRDKEFLSVQEAAILLGASRWT
                     IQRMIQRDELKAGKLGRRTIIPRSEIDNLFN"
     gene            1756..2862
                     /locus_tag="PLE23_10240"
     CDS             1756..2862
                     /locus_tag="PLE23_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HMX83685.1"
                     /translation="MKITLRQRKKGNKITLYLDYYDQGKREYEHLGLYLTPEPEKGSL
                     TKAQKDENKKILELAESIRSKRHLEVQNSIYGFRDKEKLKGSIYEFFDALTEKKKASL
                     GNYGNWNAVRILIKAYNPKDVTFERLDKEWVEGFKDYLDYEAKTKTGDGISLNTKYSY
                     FNKLRAALKQALKEGLIKFNPAEQVEPFPQDDVEREFLTLEELQKLANTHCRNETLKR
                     MFIFSCLTGLRWSDIEKLKWSEIQHSDNLGYFIRFRQQKTNGAETLPIAEEARELLGE
                     QKDPKEKVFTGLKYGNWNKTYLKDWLIDAGINKHITFHCARHTYATLQLTLGTDIYTV
                     SKLLGHKNLSTTEIYAKVINEKKVEAANKIKIKL"
     gene            2865..3623
                     /locus_tag="PLE23_10245"
     CDS             2865..3623
                     /locus_tag="PLE23_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83686.1"
                     /translation="MEAPQIFLVEELELRISIEAVEYKHKFDNLWEEYKFDPEYFNRF
                     MEGPLLSEFVQTIYKKTQHLNNQEINKYLTVSLSQFKTHSPKLRFEAMKEQYWKDYWF
                     PNAEPNHYSSMSEYDQKYFGHVFKWYEKHFHLFQEATEQALQDFKKGYLGSFADFSIQ
                     AQQEPKNKLKTNLTVKEIAYLFRALYDEGIIDAKHKTDIFNFIAESFSSKQKEDVSAN
                     SIKNAFDVPDFNAVDFWQGKFTHLMQEAKKDKEK"
     gene            3737..4039
                     /locus_tag="PLE23_10250"
     CDS             3737..4039
                     /locus_tag="PLE23_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014022282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMX83687.1"
                     /translation="MMEVNQILEKLSQLELKIDEQNLLQKEVLNFNDACKYLDISASH
                     LYKLTSQKQIPHFCPQGKKLYFNRQEIDEWLMRNKQETSEDIETAAANYLIRNKRK"
     gene            4042..4362
                     /locus_tag="PLE23_10255"
     CDS             4042..4362
                     /locus_tag="PLE23_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMX83688.1"
                     /translation="MSFYRFSEEEVQEIKQRLEALTIEVRNKQRTEPDQVWYDNQEFL
                     QIMNISKRTAAYWRTEKVIAYSQVGNKIYYRLSDIMDLLEKSTIPAKFTNLNFSTDDD
                     NNDL"
BASE COUNT         1586 a          879 c          850 g         1146 t
ORIGIN      
        1 ttcatgtcat ggttcgccat atattcagca acagatcatc ggattgtttc ttgcagatgg
       61 tgtcaggctg gcaactccgg gtgagtacac catgcgtgca ttcctcaacg ggaggatgga
      121 cttatcgcag gcagaagctg tagccgacct gatagcagct gagagtgcca ccggtcatca
      181 gatagccatg agacagatgc gtggcggatt ttcggatgag ttgtccgggc tacgtgaaga
      241 actggttaat tttgccagcc ttatagagtt ggagcttgac tttggggagg aagatgtaga
      301 atttgccaat cgatctcagc tcgtcgaatt gctcgaaaaa atcattcaca caatagacgg
      361 aatgatatac tcatttgagt tgggcaacgc catcaaaaac ggtgtcaata cggtcattgc
      421 cggacggccg aatgccggta aatcgacttt gctcaatgct ttgctcaatg aagaaagagc
      481 tattgtgtct gagatagcag gtaccacccg cgacaccatc gaagaggtac tcatccttga
      541 aggcatccga tttaggctga tagatactgc cggcatacgc gaagcccagg atcagataga
      601 agccataggt gtcagaaaga catttgaaca ggcaagtaaa gcctcaatcc taatatacgt
      661 atttgacgtt acagaatgca gccctgagca agtacatgaa gaccttgaaa aactgaacca
      721 tccgaacgaa aagactcttg taatagcgaa taagatggat ctgaatccat acgctaagcc
      781 agaaaattat gcaattgggt cggtctcagt tgataacatc ataacagcat ctgccatcca
      841 caagatgaat attccttatc tgatgagccg tatcacttcc ttggttaagt caggagaagt
      901 aacagcgggt tcgacgatca ttagcaacag ccgtcatttg gagtcactga ctgcggcacg
      961 tgagagtctg cacaaggcca aagagaacat acaagcaggt gtaacaggtg atttcatagc
     1021 aatggatatc agaaatgccc tttatcatat tggcagcatt acaggaaaga tagatgttga
     1081 ccaagatata ctaggcacca tttttggccg gttttgtatc ggaaagtagt tatccttctc
     1141 ttatttgcta actacctact attgttaggt ttataggttt actttcttct cttttttgtt
     1201 gcccaattgc actttgttgc ttatatttgt tgcccagttg ttgctcaaac aacaaatttg
     1261 ttgcccaaag gcacaaattg aaaggcactg gtgaaatgat gcaccaatca gcaccaacga
     1321 gcaacatcag gcactaataa gcaccaaatg agtagtaata ttaaaattcc aaaattctgt
     1381 caatattgtg gtcaggcatt tgttgcccaa aaaactacaa ctcgcttttg tagtcacaaa
     1441 tgtgcaagta gagcatacaa acaaagaaag cgagaagaga aagtaggttc tactctaaaa
     1501 gagcaaatca aatccgttac ttctgcaaat tccgaaaacc tgcaaagtct gcaaagcctg
     1561 ccaattaaaa caggcaattt tatcaatctt agagataagg agtttttaag cgttcaagaa
     1621 gctgccattt tattaggtgc aagcagatgg acaattcaac gaatgattca aagagatgaa
     1681 ttaaaagcag gtaagttggg cagaagaacc attataccac gttccgaaat tgataatctt
     1741 tttaactgat gagcaatgaa aataacatta agacaacgca aaaaggggaa taaaataacc
     1801 ctctaccttg actactacga ccaaggcaag cgagagtatg agcatttagg tttatacctc
     1861 acacccgaac ccgaaaaagg aagtttaacc aaagcccaaa aggacgagaa caaaaaaatc
     1921 cttgaattag ctgaaagcat ccgaagtaaa agacatttag aagttcaaaa cagtatttac
     1981 ggtttccgag ataaagagaa attgaaaggc agtatctatg agttttttga tgcccttaca
     2041 gaaaagaaaa aagccagttt aggtaattat ggcaattgga atgcagtccg cattctgata
     2101 aaagcctaca accctaaaga tgtaaccttt gagcgattag ataaagaatg ggttgaaggt
     2161 tttaaagatt acctggacta tgaagccaaa accaaaacag gtgacggaat ttcactcaat
     2221 acaaaataca gctatttcaa caaattgaga gctgcactta aacaggcatt aaaggaaggc
     2281 ttaatcaaat tcaatcctgc tgagcaagta gaaccctttc cgcaggatga tgtagaacgt
     2341 gaatttttaa cccttgaaga attacagaaa ttagccaaca cccattgccg aaatgaaacg
     2401 ctaaagcgta tgttcatttt ttcttgtcta actggtttaa gatggtcaga tattgagaaa
     2461 ctaaaatggt cagaaattca gcactctgat aatttaggct acttcattcg ttttcgtcaa
     2521 caaaaaacca acggagcaga aactttaccc attgccgaag aagcaagaga attgttaggc
     2581 gaacaaaaag acccaaaaga aaaggtattt acagggctta aatatggcaa ctggaacaaa
     2641 acctatttaa aggattggtt aattgatgca ggcatcaata agcacatcac gtttcactgt
     2701 gctcgtcata cttacgctac gcttcagttg actttaggca cagacattta caccgtttca
     2761 aaattattag ggcataaaaa cctctctact accgagatat acgcaaaggt tatcaacgag
     2821 aaaaaggtag aagctgccaa caaaatcaaa atcaaattat aaggatggaa gcaccgcaaa
     2881 tatttttagt agaagaactg gaattgagaa tttcaattga agcagttgag tacaaacata
     2941 agtttgacaa cctttgggaa gagtacaaat tcgaccctga atatttcaac cgatttatgg
     3001 aaggtccttt attgagtgaa tttgttcaaa ccatttacaa aaaaactcaa cacctcaaca
     3061 atcaagaaat caataaatac cttacggtat cattaagcca gtttaaaacc catagcccaa
     3121 agctacgctt tgaagctatg aaagaacagt attggaaaga ttattggttt ccaaatgcag
     3181 agccaaatca ttattcctca atgtccgaat atgatcaaaa gtatttcggt cacgttttca
     3241 aatggtatga gaagcatttc cacctcttcc aagaagctac agaacaagcc ctacaagact
     3301 tcaaaaaagg ctatttaggt tcttttgctg acttctctat tcaagcccaa caagagccga
     3361 aaaacaagct aaaaactaat cttacagtca aagaaatagc ctatttattc agagcattat
     3421 atgatgaagg tatcattgat gccaaacaca aaaccgatat ttttaatttc attgcagaga
     3481 gtttttcatc taagcaaaaa gaagatgtta gtgccaactc tatcaagaac gcttttgatg
     3541 ttccagactt caatgcagtt gatttttggc aaggaaaatt cactcattta atgcaggaag
     3601 caaaaaaaga taaagaaaaa taaacaacat aaccaactga taataaacac aataacatag
     3661 ggttgcacaa tcctatgcaa ctaccccagc actcataaag tgccttgttt tgcttccata
     3721 atcataaaaa tattaaatga tggaagtaaa tcaaattctc gaaaagctaa gtcaattaga
     3781 actaaaaatt gacgagcaaa acctacttca aaaagaagta ctcaatttta atgatgcctg
     3841 caagtatctt gacatcagtg catcacatct ctacaagcta acctctcaaa agcaaatccc
     3901 acatttttgc ccacagggaa aaaagctcta tttcaaccgc caagaaatag acgaatggtt
     3961 aatgcgtaat aagcaggaaa cttcggaaga catcgaaacg gcagcagcta attatttaat
     4021 cagaaacaaa cgtaaataat tatgagcttt tatagatttt ccgaagaaga ggttcaagaa
     4081 atcaaacagc gtttagaagc cttgaccata gaagtacgca acaagcaaag aacagaacct
     4141 gaccaagttt ggtacgacaa tcaggagttc ctccagatta tgaacataag caagcgtacc
     4201 gcagcttatt ggcgtaccga aaaggtaatt gcctactctc aggtaggtaa taaaatctac
     4261 taccgccttt ccgacattat ggatctactc gaaaagagta ccatacctgc aaaattcact
     4321 aacctaaatt tttctactga tgacgacaat aacgacttat aagaatttca atatacctat
     4381 tgaggacgtt tcactttcct caatcattta taatatcaaa tccggcactt accacgatag
     4441 cataaatgct attcgtatgg c
//
LOCUS       DAOQQY010000236         4461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9099_length_4461_cov_5.434635, whole genome shotgun sequence.
ACCESSION   DAOQQY010000236 DAOQQY010000000
VERSION     DAOQQY010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9099_length_4461_cov_5.434635"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="PLE23_10260"
     CDS             complement(<1..291)
                     /locus_tag="PLE23_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83689.1"
                     /translation="MLRTNLIKAIMVLCTLSASYNAFAQEQNVVEKSANTAIQYSAST
                     RHMWEVGLNGGLNFLTGDISWKPGFGGGIHIRKAIDNMFSLRLDGQYRFMKGF"
     gene            complement(363..1598)
                     /locus_tag="PLE23_10265"
     CDS             complement(363..1598)
                     /locus_tag="PLE23_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HMX83690.1"
                     /translation="MSDMLEKYKNKTPEVVFEWNDDQTGARGWTVINSLRNGAAGGGT
                     RMRKGLTKDEVTSLAKVMEIKFSVSGPSIGGAKSGIDFDPADPRKDGVLKRWFKAVFP
                     LLKNYYGTGGDLNVDEIRDVIPITEEYGLWHPQEGVVNGHFGASQGNAVKMIGQLRYG
                     CVKPVEDNNYIVDGYYKYAVADLITGWGVSESVRHYYDIFRNSDLKGKTAIIQGWGNV
                     ASAAALYLAHNGVKIIGILDRDGGVINKKGFSFDDVKSMFINKSGNKLNVKDIKPFDQ
                     INAEIWSLGADIFVPGAASKIVTRQQAEQLLKGNIEVIACGANVPFEDDGVFFGETAH
                     LLDKQIALIPDFISNCGMARVFAYLMQPDVVITDQAIFQDVSETIHKALREVHISHKE
                     PVKISENALNIALKKLLAD"
     gene            1723..2814
                     /locus_tag="PLE23_10270"
     CDS             1723..2814
                     /locus_tag="PLE23_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83691.1"
                     /translation="MLENWIESPDPGISKGCETETIGHFLSENSNIFESLESIKIAII
                     SDKHPGNAHWRKAFYNLFNHFPNLHFADLGSLRKNNKECLTPVIKELLSGGIFPIIIA
                     HDAALSEAMLVAYKTFGDKFKLLVADEKIRLSKTKNKKSYLNKLNEPDVRAYMIPSII
                     GHQIHFNSPEVLGLSEDLFYDQIRLGNLINNLSDAEPIIRDTDSFLFHLSAIKSSDAP
                     AVAEPSPNGLSGFESCQLMRYAGFSDKLTSLGLFGLEHKNDLANQTAALIAQMIWYLI
                     DGFYHRLKEFPISTANMQAYIVEQKHSDYSLKFWKSKISGRWWLQVPDKEVNSKGQHN
                     LFPCTQSDYEEAINGEIPDRLFFALQKNI"
     gene            complement(2967..4274)
                     /gene="murF"
                     /locus_tag="PLE23_10275"
     CDS             complement(2967..4274)
                     /gene="murF"
                     /locus_tag="PLE23_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMX83692.1"
                     /translation="MMHTLDIYKRFLECDSLSTDTRSIVPGCMFWGLKGETFDGGEFC
                     EQAIEKGARYAVRQAEGISANPQIIHVADSLFALQELARHHRRSFGIPVMAVTGTNGK
                     TTTKELLNAVFAAHYKTHCTKGNLNNHIGVPLTLLAMKQDVDIAIIEMGANHVGEINQ
                     LCTIAEPTCGLITNIGKAHLEGFGSLEGVKRAKSELYQYLDSVGGVIFVNMDEAELVE
                     SSMPYRRKIPYQKGVVQPAENFPSWVGLESGNGFLEVSFRSTDYGRINVQTQIVGEYN
                     FNNIMTAIVVGQYFKVPDQQIKSSLENFIPANNRSQIIQRDNYKILLDAYNANPSSMT
                     QALNNFTKIHAQRKIVILGDMFELGEYSRAEHQSIADLVSASDFEASVLVGKEFAKTR
                     TDQHVLKFTEISDLKQWFDNLEKDEATILIKGSRGMKLETLLA"
BASE COUNT         1300 a          965 c          798 g         1398 t
ORIGIN      
        1 aaatcctttc atgaatctgt actgaccatc aagtctgagc gaaaacatat tatcaattgc
       61 cttgcggata tggatgccgc ctccaaagcc gggtttccat gatatatcac ctgtcagaaa
      121 attcaagcca ccatttagcc ctacctccca catgtgtcgg gtagaagcag aatactgtat
      181 ggctgtattt gccgattttt cgacaacatt ctgttcttgt gcaaaagcat tgtagcttgc
      241 actcagcgta cacagaacca ttattgcctt gattaagtta gttcttaaca tggttgtgtt
      301 ttttttaatt ggtcaaaata aaaatatcta caataatgcc tgaccgaaaa ataaataatc
      361 tcctaatcag ccaatagttt tttcaacgca atattcagtg cgttttctga gattttaacc
      421 ggttctttgt gggatatatg aacttcgcgt aatgccttgt ggattgtttc tgagacatcc
      481 tgaaaaatgg cttggtctgt gataacaaca tcgggttgca tcagataggc aaagaccctg
      541 gccattccgc aatttgatat gaagtcggga ataagtgcta tttgtttgtc taaaaggtgg
      601 gcagtttcgc caaaaaagac accatcatct tcgaaaggta cattggcacc gcaggctata
      661 acttctatat tgcctttcag taattgttct gcctgttgcc ttgtgacgat tttgctagcc
      721 gctccaggca caaaaatatc agcaccaagg ctccatattt cagcattaat ctgatcgaat
      781 ggcttaatgt ccttaacatt gagtttattg ccggatttgt tgatgaacat tgattttaca
      841 tcatcgaatg aaaacccttt cttgtttatg acaccaccat cacgatccaa tattccaatt
      901 attttgacac cattatgcgc cagatacaag gctgcagcag atgcaacatt gccccaaccc
      961 tgaatgatgg cagtcttccc ttttaggtct gagtttctaa aaatatcata ataatgtctg
     1021 acagactctg atacgcccca tccggtgatg agatcggcaa cagcatattt ataataacca
     1081 tcaacaatat agttattatc ctctacgggt ttgacacaac cataacgcag ctgaccaatc
     1141 attttcacag cattgccttg gctggcacca aaatgcccgt taactacacc ttcctgagga
     1201 tgccataggc catactcttc ggtgatgggt ataacgtccc ttatttcgtc aacatttaaa
     1261 tcacctcctg taccatagta atttttcaat aatggaaata cagctttaaa ccatcgttta
     1321 agcactccat ctttacgtgg gtcggccggg tcaaaatcaa ttcccgattt tgcaccgcca
     1381 atggaaggac ccgatacaga aaattttatc tccatgactt tagccagtga tgttacttca
     1441 tctttggtaa gcccttttcg catgcgagta ccaccacctg cagcaccatt cctgagtgag
     1501 ttaatcacag tccagcctcg tgccccagtc tggtcatcgt tccactcaaa taccacctcg
     1561 ggtgtcttgt ttttgtattt ttccaacata tcactcatat cactctatta ctttcaatcc
     1621 caaaaataaa ctatccatcg gtttttttat atcttttata tagcattttt ttgtcatgtc
     1681 caaaatcatc cctaatattg tgacttatct gtattaattt ttatgcttga aaactggata
     1741 gaaagcccgg atccgggaat atctaaagga tgtgaaactg aaaccatcgg acattttttg
     1801 tcagaaaata gtaacatttt tgaatcactc gaatctatta aaattgcaat cataagcgat
     1861 aaacatcccg gaaatgcaca ttggcgtaaa gccttctaca atttgttcaa tcatttccca
     1921 aatcttcatt ttgctgatct gggatctttg cgaaagaata ataaggaatg ccttactcct
     1981 gtaatcaaag agcttttatc aggtggtatt tttcctatca tcattgccca tgatgcagcc
     2041 ctgagtgaag caatgctggt ggcttacaaa acatttggtg acaagttcaa actcttggta
     2101 gcagatgaaa aaatccgatt atctaaaaca aaaaacaaga aaagctacct aaacaagctg
     2161 aatgaacctg acgttcgcgc ctacatgata ccaagtatca taggtcacca aattcatttc
     2221 aactcacctg aagtgctcgg cttatctgaa gatttatttt atgatcaaat caggcttggc
     2281 aaccttatta acaatctttc tgacgccgaa cctataatcc gagacacaga ttcttttcta
     2341 ttccatctta gtgccatcaa atcatcagat gcgcctgcag tagcagaacc ttcaccgaac
     2401 ggattatccg gatttgaatc atgccaactc atgcgatatg ccgggtttag tgacaaatta
     2461 acttctttgg gtttgtttgg gttagagcat aaaaatgatc tggcaaacca gactgctgcc
     2521 ctgattgctc agatgatctg gtatctgatt gatggtttct accaccgtct aaaggagttc
     2581 ccgatatcaa cggccaatat gcaagcctac atcgtcgagc agaaacattc tgattattcg
     2641 ttaaaattct ggaagagcaa aatctcagga agatggtggc tacaggtacc cgacaaagag
     2701 gtaaattcga aaggtcaaca taatcttttc ccttgcacac aatctgatta tgaagaagct
     2761 atcaatggcg aaataccgga tagattattt tttgcacttc aaaaaaacat ttaaccagat
     2821 taacgtagta ggaattcaaa caaaatgata agcctaaaaa aaccttaaaa agtaaaaaaa
     2881 gcatagtgag ctacagtttt tgaacttttg aaatggattt gaagtaataa taatttgcag
     2941 taaagatgcc aatactttat ccgggtttaa gcaagtagtg tttcgagctt catcccacga
     3001 gatcctttta taagtatggt ggcttcgtct ttctccagat tatcgaacca ttgcttcaaa
     3061 tcagatattt cagtaaactt caatacatgt tggtctgttc ttgtttttgc gaactcttta
     3121 ccgactaaaa ccgatgcttc aaaatccgat gcacttacca aatctgcaat agactggtgt
     3181 tctgccctgc tatactctcc aagttcaaac atgtcaccaa gtattactat ttttctttgt
     3241 gcatgtattt ttgtgaaatt gttgagtgcc tgtgtcatac ttgatgggtt ggcattatag
     3301 gcatcaagta gtattttgta gttatcacgt tgaattattt gagacctgtt gttggctgga
     3361 atgaagtttt cgagtgaaga tttgatctgt tggtctggta ctttaaagta ttgtccgaca
     3421 actattgcag tcatgatgtt attgaaatta tattcaccca caatctgagt ctgaacattt
     3481 atgcgcccgt agtcagtaga gcgaaaactt acttcaagaa acccatttcc ggattcaaga
     3541 cctacccatg aaggaaaatt ttcggcaggc tgtaccacac ctttttgata gggtattttc
     3601 cgtctgtatg gcatggagct ttctacaagc tcagcttcat ccatatttac aaatatcacc
     3661 ccaccaacac tatcaagata ttgataaagt tcagattttg ctctttttac accttcaagg
     3721 ctaccaaaac cttccagatg tgctttgcca atattggtaa tgaggccgca tgtaggctcc
     3781 gcaatggtgc acaattggtt tatttcaccg acatgatttg ctcccatttc aataattgca
     3841 atgtcaacat cctgcttcat ggcaagcaat gtaagtggaa caccgatatg gttattcaga
     3901 tttcctttgg tacaatgcgt tttataatgt gcagcgaata cagcattaag caattcttta
     3961 gtagtagtct tgccattggt acccgtgact gccattaccg gaataccaaa acttctccta
     4021 tggtgtcgtg ccaattcttg aagtgcaaaa agtgaatctg caacatggat aatctgtgga
     4081 tttgcagaaa tgccctctgc ctgccgtaca gcatagcgcg cacctttctc tatagcttgt
     4141 tcgcagaatt ctccaccatc aaatgtttct cctttcaggc cccaaaacat acaacccggt
     4201 actatacttc tggtatctgt tgaaagtgaa tcgcattcca aaaaccgctt gtaaatatcc
     4261 aatgtgtgca tcataatttt attaataaaa aaaagtctcc aaagataaac cttggagact
     4321 tttctgtttg agttaacaac agattattta tattttctct gtactttttg ctttttagct
     4381 ttgaacttgt tacctgcgct atcgtcgtta ccggcagggc caccggttct tgtcatggcg
     4441 catctgaaac caagcgtacg g
//
LOCUS       DAOQQY010000237         4457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9112_length_4457_cov_5.446388, whole genome shotgun sequence.
ACCESSION   DAOQQY010000237 DAOQQY010000000
VERSION     DAOQQY010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9112_length_4457_cov_5.446388"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLE23_10280"
     CDS             complement(<1..306)
                     /locus_tag="PLE23_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMX83693.1"
                     /translation="MNLSTILIENQNNVIILTINREASLNALNKSVFDDLDYFFTKGH
                     LDFPHIKGVVITGSGTKAFAAGADIKEFMDFTAEQAQALSKRGHDVFDTIEGFHVPVI
                     "
     gene            complement(371..1864)
                     /locus_tag="PLE23_10285"
     CDS             complement(371..1864)
                     /locus_tag="PLE23_10285"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMX83694.1"
                     /translation="MGIIIKQSLKYSFINYIAVLIGVISTVFIYPLDKQVYGLFRFLI
                     DTSYLLVPAVLIGTPALSVKFFPVFKGNKHKEGEFITQLYKLALIGFILVCLVIFAFR
                     KPIIDYYGQKPERQYLEYLWFIIPFLFANAVFGLSRQIVANYGKIVWPSALEGSIKIV
                     FPALFLAYYWHYIHLDWVIMGVGIFLFTIMALSLWYVMKSGAVKLKLPKTTTWGIEKR
                     EFYRYAAYFTLGSAGAIIATRIDTFMVASMIDLNRTGIYSIAALVALNIGIPINAVTA
                     ISAPIISQAISVNNWKEVEKIYKKSSITLLVAGCFLFLLVWVNLDDLFGLMPDNDEMI
                     AHKDIVLLLAVAKLFDMATGVNDSITAYSGHYKLNFYSLLLLAILNIVGNLLLIPQYG
                     LLGAAISPMIASVIFNLVKLVYIKWAFNMYPFTVKTFVLILISVAAYILLYFVPDFEN
                     HFINLCINSALLAAIFVPAIYFSKISEDVNSILDNIISKALKLLKLK"
     gene            complement(2186..>4457)
                     /gene="dnaE"
                     /locus_tag="PLE23_10290"
     CDS             complement(2186..>4457)
                     /gene="dnaE"
                     /locus_tag="PLE23_10290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMX83695.1"
                     /translation="IDMPADDRNNRQSFTGSKNGDMSLLTKLTENGAAKRYRGKVLAK
                     AVARIQKELGVIAQLGFAPYFLITWDIIRYGQSVGYNHVGRGSGANSIVAYCLYITDV
                     DPLELDLYFERFINPYRTSPPDFDIDFSWSDRDDVTDYIFKRYGKEYTALLGTYNTFK
                     GRSILREIGKVAGLPKPEIDLMVKQPLARDRHHPLAESIFKYGKLIEGFPNYLSIHAG
                     GVLISERPLNYHTALQMMPKGFPITHFDMYGAEDLGFHKYDILSQRGLGHIRDTVELV
                     RQNRNETVDIHDMPKIKEDEKVKAQLRSGQCIGCFYIESPAMRGLLSKLRCDNYTHLV
                     AASSIIRPGVAKSGMMREYIQRFHRPESFEYIHSVFAQHLGETFGIMVYQEDVMKIVH
                     HFAGLDLDESDILRRIMSGKKKTSDTFERLMTKYFENCRQRGYPDELAQEVWRQVESF
                     SGYSFCKAHSASYAAESFQSLFLKSYYPLEFMVAVINNFGGFYHTELYVHEARMQGGN
                     IHAPCVNHSRYLTTIDGNDIWLGFIHLNGMERKIAQAITNERERDGHFLSLADFTSRV
                     DIASDQLDLLIRIGAFRFTGYNKYELMWEKNAVYNPEVKVCEGIRLFREEAETYTLPV
                     LAEGPFDQAFDEIELLGFPLCSPFELVENLPRQYIAAEEMLRHAGRHVYMLGYYVTTK
                     PVTTIKRELMQFGTWVDTKGQFFDTVHFPDSLKKFPFKGRGVYYICGRIVLDFGFPNL
                     EVDRMEKLPYVKDVRY"
BASE COUNT         1351 a          947 c          924 g         1235 t
ORIGIN      
        1 tataaccggc acgtggaatc cttctattgt gtcaaataca tcatgccccc ttttagagag
       61 agcctgtgcc tgctcggcag taaaatccat aaattcctta atgtctgcgc ctgcggcgaa
      121 agctttagta cctgaaccgg taatgaccac acctttgatg tgtggaaaat cgagatggcc
      181 ttttgtaaaa aaataatcca gatcatcaaa caccgactta ttgagtgcat tgagagaagc
      241 ttcacggttg atggtcagga taatgacatt attctggttt tcgattaata tggtagaaag
      301 attcatgttg ataaaatatt tcaggcaaaa atagttaatt aaatccaatc ggaaataata
      361 ccgtgagatt ctacttcaat tttaataatt tcaaggcttt agagattatg ttatccagaa
      421 ttgaattaac atcttcagat atttttgaaa aataaatagc aggtacgaaa attgcggcaa
      481 gcaaagcaga attgatgcaa agattgataa aatggttttc aaaatcagga acaaaataaa
      541 gtaagatata agcagcaaca gatataagaa taagtacaaa ggtcttaacg gtaaacgggt
      601 acatattgaa agcccacttt atatacacaa gtttgacaag attaaagatg accgaagcta
      661 tcattggcga aatagcggca ccaagcagac cgtattgggg gatcaaaagc agattgccga
      721 cgatattaag tatagccagc aacaacagtg aataaaaatt aagcttgtag tgccccgaat
      781 aagctgtaat agaatcatta acacccgtag ccatgtcgaa aagtttggca acagcaagca
      841 gtaaaacgat gtctttatgc gcaatcattt cgtcattatc cggcatgagt ccgaacaagt
      901 catcaagatt aacccaaaca agtaaaaaca agaaacaacc ggctaccaac aaagtgatgg
      961 atgatttttt gtatattttt tcgacttctt tccagttatt gacagaaatg gcttgtgaaa
     1021 tgataggtgc agaaatagct gtaaccgcat tgataggtat gccgatattg agtgccacaa
     1081 gtgcagcgat tgaatatata ccggtgcgat tcaggtcaat catggatgcc accataaacg
     1141 tatcgatgcg ggtagcgatg atggcaccgg cactaccaag ggtaaagtaa gctgcatagc
     1201 gataaaattc tcttttctcg attccccatg tagttgtttt gggtaatttt agcttaacgg
     1261 caccggattt catcacatac cacaaagaca aagccattat ggtaaagaga aaaataccaa
     1321 cacccattat tacccagtca agatggatat agtgccagta ataagcaaga aacaaggccg
     1381 gaaaaacgat tttaatggag ccctccaaag ctgaaggcca cactatcttc ccgtagttgg
     1441 ccactatttg cctgctaagg ccaaaaacag cattggcaaa aagaaacggt ataataaacc
     1501 agagatattc aagatactgt ctttcaggtt tttgaccata ataatcaata attggctttc
     1561 ggaaagcaaa aattacaaga cataccaata taaaaccgat gagtgccaat ttataaagct
     1621 gtgtaataaa ctcgccttcc ttgtgtttat tgcctttaaa aaccggaaaa aatttaacac
     1681 taagagcagg tgtgccgatt aatacagccg gtacaagcag gtaagaagta tctatcaaaa
     1741 acctaaacaa gccatagact tgcttgtcaa gcggataaat aaacaccgta ctaataaccc
     1801 caatcagaac ggctatataa ttgatgaagc tgtacttcag actttgtttg ataatgattc
     1861 ccatttgggt actatattgg tgtcaaagat aaaaaatagt ggattgagca gaaaaattat
     1921 ttccgttaaa ggcaaattgg gctttattta caacgaaatt taatctgagt tatgcaatcg
     1981 ggcataattc ttatctgtaa tagactcatt ttgaccaagg taatacttat ttgaatttca
     2041 ccaatttggt taatcttctc atagagtata gtgccaagtc tgtcactcca atatgcatat
     2101 ttcaggttaa acaaaaaatg gcaaaacgga ataagtgcac tatacaagga agcgtctgct
     2161 tcatattcca aaaaaatatc gtccctcaat accgtacatc ttttacataa ggcagttttt
     2221 ccatccggtc cacttcgagg ttggggaatc cgaagtcaag gacaatccgg ccacagatat
     2281 aataaacgcc ccttcctttg aacggaaatt tctttaatga atcggggaaa tggactgtat
     2341 cgaaaaactg ccctttcgta tctacccatg taccaaactg catcagctcg cgtttaatgg
     2401 tcgtcacggg cttggttgtc acataatagc ccagcatata tacatgccga ccggcatggc
     2461 gtagcatttc ttcagctgct atgtattgtc ggggcagatt ttctaccagt tcaaaaggag
     2521 aacacaaggg gaagccaagt agctcaattt cgtcaaatgc ctgatcgaac ggcccttctg
     2581 ccagcaccgg aagcgtgtaa gtctcagcct cttctctgaa aagtcttatg ccctcacaca
     2641 ccttcacttc ggggttgtac acagcattct tttcccacat gagctcatac ttattataac
     2701 ccgtaaaacg gaatgcgccg atgcgtatga gcagatcgag ttggtcagag gcgatgtcca
     2761 cccttgatgt aaagtctgcc agacttagaa aatgtccgtc tctttcgcgc tcatttgtga
     2821 tggcttgagc aattttcctt tccatgccgt tcagatgtat gaagcccagc catatatcat
     2881 taccgtcaat ggtagtcaga taacggctgt ggttaacgca gggtgcatga atgttgccgc
     2941 cctgcatgcg agcctcatgt acatagagtt cagtatggta aaatccgcca aaattattga
     3001 ttactgctac cataaactca agtggatagt acgatttcag gaaaaggctc tgaaatgatt
     3061 ctgcagcata gctcgctgag tgtgctttac aaaaagaata tccgctgaag ctctctacct
     3121 gccgccatac ttcttgtgcg agctcatcgg ggtacccgcg ctgacggcag ttttcgaagt
     3181 atttggtcat gaggcgttcg aatgtatcag atgttttttt cttgccgctc attatccgtc
     3241 tgagtatatc cgattcatcc aggtcgagcc ctgcaaaatg gtgtacgatc ttcatcacgt
     3301 cttcctgata aaccattata ccgaaggtct ctcccagatg ctgcgcaaac accgagtgta
     3361 tgtactcaaa actttcgggt cggtgaaatc gttgtatata ttcacgcatc atacctgatt
     3421 ttgccacacc ggggcgtata atcgagctgg cggctaccag gtgtgtgtaa ttgtcacacc
     3481 tgagttttga gagaagacct cgcatggcag gtgattcaat ataaaagcaa ccaatacact
     3541 gacctgacct cagctgtgcc tttacttttt catcttcctt aatcttgggc atatcatgaa
     3601 tatccacagt ttcattgcgg ttttgtctta cgagttcgac agtatccctg atgtggccaa
     3661 gccctcgctg actcaggata tcatatttgt gaaatccgag atcttcggcg ccgtacatat
     3721 caaaatgtgt aatcgggaag cccttgggca tcatttgtag tgcagtgtgg taatttaacg
     3781 gtcgctcact gatgagcaca ccacccgcgt gtatagaaag gtaattgggg aatccttcaa
     3841 tcagttttcc gtatttaaaa atcgattctg ccaacggatg atgtctgtcg cgggcaagtg
     3901 gctgctttac catcaggtct atttctggtt taggcaatcc ggccacttta cctatctcgc
     3961 gcagtataga tcttcctttg aatgtattgt aagtgccaag gagtgcagtg tattccttgc
     4021 catagcgctt gaatatatag tcggtaacat catcgcggtc agaccatgaa aagtcaatgt
     4081 caaaatcggg tggtgaagtc cggtatgggt taataaaccg ttcgaaatag aggtcgagct
     4141 caagcggatc gacatcagta atatacaagc aatatgccac aatagagttg gcaccactac
     4201 cccgccctac atggttatag ccgaccgact gtccgtagcg gatgatgtcc caggtaatga
     4261 gaaaatacgg tgcaaaacca agttgggcaa tcacgcccag ttccttttgt attctcgcta
     4321 cagccttggc cagtactttg ccgcgatacc ttttggctgc tccgttctca gtgagtttgg
     4381 tcagcagact catgtcgccg tttttacttc ccgtaaaact ctgtctgtta ttcctgtcgt
     4441 cagcaggcat atctatt
//
LOCUS       DAOQQY010000238         4396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9302_length_4396_cov_6.028334, whole genome shotgun sequence.
ACCESSION   DAOQQY010000238 DAOQQY010000000
VERSION     DAOQQY010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9302_length_4396_cov_6.028334"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLE23_10295"
     CDS             complement(<1..434)
                     /locus_tag="PLE23_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83696.1"
                     /translation="MDIVVKKHFESKGHRSPVYGLAHYVSGQILSGGGEGWITSWDIA
                     DPENGRLIAQANTSVFCLNYFEPAKLILAGDRNGVLYFIENTGNVVKRYAHQGEIFAL
                     NVVGDYLYSIGKDGWLVKWDLHRMDALQSFQLSYTSLRSFVHS"
     gene            491..1123
                     /locus_tag="PLE23_10300"
     CDS             491..1123
                     /locus_tag="PLE23_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HMX83697.1"
                     /translation="MIQKAIHAHTILAWSELEEVPGCNKDDEAFVREKNIHHPLKQKQ
                     FYAGRRLLRSLVGAEAVINYDQYGGPIVANKRNAEVSLSHSANMVACAYSDIAIGIDI
                     QQADEKFERVKFRFMSSDELSESEKTNRADYVQFCWSAKEAMFKAFRKGNINFRQQLM
                     PNIPLDELDLDEFESTGHLIIGNKLIAMRLNYYKHDSYTIVSASFDASKY"
     gene            1127..1561
                     /locus_tag="PLE23_10305"
     CDS             1127..1561
                     /locus_tag="PLE23_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83698.1"
                     /translation="MKKLLFVILAFSMLISCKEKQKKITGETSGNLPAGFEAFYKKFH
                     QDSFYQIEHCVFPMEGLPDQADSSLNTAGFRWTPENWRMHKELSDTSSGMKRTWDIIS
                     DQMVNERMLNEQQGIGMERRFAKMNDEWYLIYYVGVNQYRKH"
     gene            complement(1577..2155)
                     /locus_tag="PLE23_10310"
     CDS             complement(1577..2155)
                     /locus_tag="PLE23_10310"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HMX83699.1"
                     /translation="MAANILPFDGEIFYFPDVLDADSERLYCQVIKDETPWQQQSVKI
                     FGKWIPEPRLTCFYGDAGKCYSYSGLRLEPLAWTPALLQLKNLSESYSGTLFNVALLN
                     YYRDGKDSMGWHRDNEPELGTEPIIASFSLGAARKFSLRNFADKSVKQSIILEPGSLL
                     VMRGKSQSCWEHCVPKKSKAEERINITFRRII"
     gene            2337..3389
                     /locus_tag="PLE23_10315"
     CDS             2337..3389
                     /locus_tag="PLE23_10315"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HMX83700.1"
                     /translation="MYRYFYTVVLGILLGSCSSHTTAIRALKPATITLPDHIQSIGTI
                     DRSMPEKGFSGILEGALTGEQIGQDKRGRQAAIEGFAKTLTQTPRFSIRPIATELTGS
                     KDGSTMSRPLAWTEIDKLCKMYQVDAIAALELFDSDQFFSKSSKNESYKDKEGKEHSR
                     LRYYIDRKKEVRTGFRVYDNVNKDIIDEFVTSGQNSDSGNGLTETDAQNNLRNVYDQV
                     REVGTTSGRIYAQRIAPLYETIHRSFIKKVPTNKDAHEKALNAAKAKDWTKAAEIWNT
                     MASQSTDVKTKGIAAYNMAIVCEAQGKIDLAIDWAKKSYYEHGYKKASRYLVQLKDRK
                     YDLERLEIQMKSRNNT"
     gene            3448..4206
                     /locus_tag="PLE23_10320"
     CDS             3448..4206
                     /locus_tag="PLE23_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83701.1"
                     /translation="MKYLQKLIFITSINLFCLYCIGQNTYTVQSDINSVRQKFTTLTN
                     SDTYAQIRFRNATTGQYFTPQSVIITEKSTAQIYQHIARQDSNAAERAQQTDNKILRE
                     YTIHGGTVKFRVKEHCDYIISVKDPQYSNAEISFNWLELIGNNVVIANVSPILELMLN
                     GVVTADGKYYQKTELVITYQDEQTRQLQRASFKPDGTCELLVKFGAQYLLKIKQPSNK
                     EVSFDLEIPSDIKLFRPVTLKSGLIELYLDIARK"
     gene            4193..>4396
                     /locus_tag="PLE23_10325"
     CDS             4193..>4396
                     /locus_tag="PLE23_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83702.1"
                     /translation="MPENKPVSATPAIREEANTENAEDFFLKNSGDLHITKGQTFKLS
                     NYQFDYQAHILTDKCMYELDLLTQ"
BASE COUNT         1484 a          871 c          887 g         1154 t
ORIGIN      
        1 gaatgaacaa acgaccttag cgaagtgtaa cttaactgaa agctctgtaa ggcatccatt
       61 ctgtgaagat cccattttac aagccagccg tcttttccga tagaataaag gtaatcacct
      121 actacattca aagcgaaaat ttcaccttgg tgagcatacc ttttcactac attacctgta
      181 ttttcaataa agtaaagtac accattcctg tcacctgcaa ggatgagttt ggcaggttcg
      241 aaatagttca ggcaaaatac agaagtgttt gcctgtgcga tcaagcggcc gttctcaggg
      301 tcagctatat cccagcttgt gatccagcct tcaccaccac cggacaatat ttgaccacta
      361 acataatgcg caagcccata aaccggtgaa cgatgcccct tgctttcgaa gtgtttttta
      421 acaactatgt ccatgcaaaa ttgaagattt ttgtaaaatt tggctaattt tgtgatgtaa
      481 aaatacggat ttgattcaaa aggcaattca tgcacataca atcctggcgt ggtctgaact
      541 agaagaagtt cctggatgta acaaagatga cgaggcattt gttcgtgaga aaaatattca
      601 ccatcctttg aagcaaaaac agttttatgc tggcaggaga ttgctccgtt cgcttgtagg
      661 tgctgaagct gtcatcaact acgaccagta tggcggaccg atagttgcca acaaaaggaa
      721 tgccgaagta tctttgtcgc attctgccaa tatggtagcc tgtgcttaca gtgatattgc
      781 catcggaata gatattcaac aggcagatga aaaatttgaa cgggtaaaat ttcgttttat
      841 gtcatctgat gaattgtctg aatcagaaaa aacaaaccgt gctgactatg tccagttttg
      901 ctggtcggcc aaggaggcga tgttcaaggc attccgtaag ggaaacatca atttcagaca
      961 acaattaatg ccaaatattc ctttagacga gttggatctt gacgaatttg aatctaccgg
     1021 acacttaata attggcaata agttaatagc aatgaggtta aattattata aacacgattc
     1081 gtacacgata gtatcagctt cctttgatgc atcaaaatat taattcatga aaaaactcct
     1141 atttgttatc cttgcattca gtatgcttat ttcctgtaag gaaaaacaaa aaaaaattac
     1201 aggtgaaaca tcgggaaatt tgcctgccgg atttgaagca ttttataaaa aatttcatca
     1261 agacagtttt taccagattg agcattgtgt tttcccgatg gaagggttgc cggaccaggc
     1321 agattcttct ttaaacactg cgggcttccg ttggacacct gaaaactggc gcatgcacaa
     1381 agagttatcc gacacatcat ctggtatgaa gagaacatgg gatatcatca gtgatcaaat
     1441 ggtcaatgaa aggatgctga acgaacaaca aggcattggt atggaaagac gcttcgctaa
     1501 aatgaatgat gagtggtacc ttatatatta tgtaggcgtc aaccagtacc gcaagcacta
     1561 aaagcccgag tgatattcaa attattcttc tgaaagtaat attaattcgc tcttcggcct
     1621 ttgatttttt tggtacacag tgttcccagc aagattgtga cttaccacgc atgaccagca
     1681 gactacccgg ttcaaggatt attgattgtt tgaccgattt gtcggcaaaa ttcctaagcg
     1741 agaattttcg tgcagctcca agactaaatg aggcgatgat cggttcggta ccaagttcgg
     1801 gttcgttatc gcggtgccag cccatactat ctttcccgtc tcggtaatag tttagcaatg
     1861 ccacattaaa tagcgttccg gaatatgatt ctgacagatt tttaagctgt aacagtgccg
     1921 gagtccatgc caatggttct aagcgcaagc cggaatatga ataacattta ccagcatcac
     1981 catagaaaca ggtgagcctt ggttcaggaa tccatttccc gaaaattttt accgattgtt
     2041 gttgccaggg tgtttcatcc tttattactt ggcaatacag ccgctcactg tctgcatcca
     2101 acacatcagg aaaataaaat atttcaccat caaaaggaag aatatttgct gccatcaaat
     2161 acaaaagtaa aacataatta aacaaatcaa accgtttcat gtagttgcaa ttggccaaat
     2221 gtggtcattg agttaaaaaa ctcaggcagt tgacggttta acagaaattt aaagccaaat
     2281 aagccgatgt ttaattgaca gataggctat aatgttataa tttgtggtca aaaactatgt
     2341 acaggtattt ttatactgtt gtccttggaa tattgcttgg gtcctgcagt tcgcacacca
     2401 ctgccattcg ggctctaaag cctgctacca tcaccttacc cgatcatata cagtcgatcg
     2461 gcactataga caggagtatg ccggagaaag gattcagcgg aatattggaa ggtgccctga
     2521 ccggagagca gataggccag gacaaacgag gtcggcaggc ggccattgaa ggatttgcaa
     2581 aaacactgac acaaacaccg agattcagta tcaggccgat agctacggaa ttgacaggta
     2641 gcaaagacgg ctccactatg agcaggccac tggcttggac agagattgac aaactttgta
     2701 aaatgtatca ggtagatgca atagctgcct tagagttatt cgattctgat cagttttttt
     2761 caaaatccag caaaaacgaa agctataaag acaaagaagg gaaagaacac agccggttac
     2821 gttattacat tgaccgcaaa aaggaagtgc gcacaggatt tagggtttat gacaatgtga
     2881 ataaagatat catagacgaa tttgtcactt ccgggcaaaa ttcggattca ggaaacggac
     2941 tcacagagac cgatgcacaa aataatttaa gaaatgtcta tgaccaggta cgagaagtgg
     3001 gcactacaag cggacgaatt tatgctcaaa ggattgcacc attgtacgaa accatacacc
     3061 gaagcttcat taagaaagtg ccaaccaaca aagatgcaca cgaaaaagca ctgaatgcgg
     3121 ccaaagcaaa agattggacc aaagcggcag aaatatggaa tacaatggca agccagtcaa
     3181 cagatgtaaa aaccaaagga atagctgctt acaacatggc catcgtatgc gaagctcaag
     3241 gaaaaataga cctggctatt gattgggcta agaaatcgta ctatgaacat ggctataaaa
     3301 aagcatcaag atatttggta caactcaagg acagaaaata tgatcttgaa aggctcgaaa
     3361 tacaaatgaa gtccagaaat aatacctaac aaattaaggc taaaaatcgg aacatttttt
     3421 gcgtaattcc gttaataagg ccagatcatg aaatatttac aaaaactgat tttcattaca
     3481 agcattaatt tgttctgctt gtattgtata ggccaaaata cttacacggt acaaagcgat
     3541 atcaattctg ttagacaaaa attcactaca cttacaaata gtgatactta tgcgcaaatt
     3601 cgtttcagga atgctacaac cgggcaatat tttacaccac aatctgtcat cataacagaa
     3661 aaatcaacag ctcagattta ccaacatatc gccaggcaag attccaatgc tgccgaacgg
     3721 gcacaacaga cagacaataa aatactcaga gaatacacaa tccatggtgg tacggtgaag
     3781 ttcagggtca aggaacattg cgattatatt atttcagtaa aagatccgca atattcaaat
     3841 gctgaaataa gcttcaattg gcttgaactg attggcaaca atgtagtcat tgccaatgtt
     3901 tcacctattc tggaattaat gttgaatgga gtcgttactg ctgatggcaa atattaccaa
     3961 aagacagaac ttgtaataac ttaccaagac gaacaaacca ggcagctcca gagagcaagc
     4021 ttcaagcctg acggcacatg cgaactactg gtaaaatttg gagcgcaata tttattgaaa
     4081 ataaagcaac ctagcaacaa agaagttagt ttcgatttag aaattccgtc agacatcaag
     4141 ctgttccgac ctgtcaccct taaatctggc cttatcgaat tatatcttga cattgccaga
     4201 aaataagcct gtttcggcga caccggctat cagagaagaa gcaaatactg aaaatgccga
     4261 agattttttc ttaaaaaata gtggagacct tcatatcacc aaaggacaga cattcaaact
     4321 aagtaattat cagtttgact accaggcaca tatacttaca gacaaatgta tgtatgaact
     4381 tgatctattg acgcaa
//
LOCUS       DAOQQY010000239         4391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9321_length_4391_cov_4.506458, whole genome shotgun sequence.
ACCESSION   DAOQQY010000239 DAOQQY010000000
VERSION     DAOQQY010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9321_length_4391_cov_4.506458"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="PLE23_10330"
     CDS             complement(<1..226)
                     /locus_tag="PLE23_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83703.1"
                     /translation="MPNANSFLRAIHTIYLMLFIFQTVILAFGSFVVFTEGTVLVDFG
                     IPVIIPGCLAIAGFLAGTFIFKKKTESLAIA"
     gene            complement(729..1631)
                     /locus_tag="PLE23_10335"
     CDS             complement(729..1631)
                     /locus_tag="PLE23_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HMX83704.1"
                     /translation="MWFKKVKFKEDLIFPTMEKPEENQWKEKTFEIIFETDTYYGRVF
                     NIALLALILINVGLVMMESVPEINARYHNILVILEWTITILFTIEYFLRLYIVRWPIR
                     YIFSLYGIIDLVSILPTYLGIFYPNTKFLASIRILRLLRISRIFRLTKFMRGGNLIMT
                     ALKRSLSEIGVFLSFITLVVVVIGALIYVIESGHPESPMKSIPTSIYWAVVTVTTVGF
                     GDITPVTAMGRFLASVLMLIGYGVIFVPTIIATETLRGTKFKDTFTNTQVCQNCHDDK
                     HLDGARFCKSCGYPLNRRDLRKML"
     gene            complement(1726..2661)
                     /gene="moaCB"
                     /locus_tag="PLE23_10340"
     CDS             complement(1726..2661)
                     /gene="moaCB"
                     /locus_tag="PLE23_10340"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF002947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HMX83705.1"
                     /translation="MIDITHKITTHRTAIAQATVRVSKAETIEAVQNRTVPKGDVLEA
                     SRVAALFAVKRTSDVIPDCHPLPIEYTSVSHHIEGLSIIISTEVHTIYRTGVEVEAMH
                     GASVAALTIYDMLKPIDKYIEIQNIRLLSKKGGKSQFRDEFDRSIDAAVVVCSDSIAG
                     GKKQDSAGKAIISKLEQHNIQVLDYTIIPDDVQTIQQKVTAFCDKGIRVVIITGGTGL
                     SAKDLTPEAVSPLIERHIPGIMEAARSYGQQRMPYAMLSRGVSGLRGDTLILTLPGST
                     RGAKESMDALFPGVLHLFKVTDWGKSDTSWPTHTS"
     gene            complement(2666..3109)
                     /locus_tag="PLE23_10345"
     CDS             complement(2666..3109)
                     /locus_tag="PLE23_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HMX83706.1"
                     /translation="METKKKHQVFVEGPIPSGFIAESIEKHSKKLNIGAHSIFLGQVR
                     NDRHENGEVGAIEYTTYHAMAEEVFHHIREETFAKYALTCMHIYHSLGTVQAGEISLF
                     VFTSSAHRADAISACEEIVERIKKEAPVWGKEILTDQKTKWKQNT"
     gene            complement(3128..4258)
                     /locus_tag="PLE23_10350"
     CDS             complement(3128..4258)
                     /locus_tag="PLE23_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HMX83707.1"
                     /translation="MTHIDKANRYQRQTSISGFGEAAQQKLARAKVLVAGAGGLGCPA
                     LLYLAAAGVGCIGIAEDDVVELSNLQRQTLYNIYDIGKPKLSCAIAHLSALNPDIKYI
                     AHSIRLDSSNALDIIEQYDLVIDGTDNFATRYMLNDACVLLDKPLVYGAVLRYEAQVA
                     VFNLKDPKTGIKTNYRDAFPHPPDIAAVTSCNEAGVLGVVPGITGSMQAAEAIKIITG
                     TGVPLCNKILSFNVLNNRYYEIEISPIKEKHSQMPDTSEKFRAWDYAWHCADKLSEYE
                     IPIARFDIMRANEDLFIIDVREPYEQPTVEGFPYTNIPLAQFEDRLEEIPEGKPIVLF
                     CKSGKRSMLAWQILQKNKTSVQAYSLKGGIDNWVQIHILQNK"
BASE COUNT         1000 a          916 c         1110 g         1365 t
ORIGIN      
        1 cggctatggc caggctttct gttttctttt tgaaaataaa agtacccgcc aggaatcccg
       61 ctatggccag gcagcccggt atgatgaccg gaattccaaa atcgaccaga actgtccctt
      121 ctgtgaaaac gacaaagcta ccaaaggcaa ggatgactgt ctgaaaaatg aacagcatca
      181 ggtaaattgt atgtatggcc cgaagaaagc tattagcgtt aggcatgttt gaatttgtta
      241 tggaaaatcg ctaagaaaat attatgacaa actcctgttt gattctgctt tcaagttttt
      301 gtatttgttt aatttccagt tggttgaatg cttgtaatga ttctgttcaa aattacggaa
      361 atgctgtttg atttgttact tttaaaagtg taaatgcata cgctaatata aatgaaacgg
      421 atttccaaat tcctaaagat cttcaatttt gtaaagtttt aggaaatgtt gggtgcatat
      481 tgtattcctg gttcgttgtt gatagggatg ccgctttttt ttggattgtt gcgtgagctg
      541 cctggagggc tttggtggct ttgccacgga gcgatagcga accccggaag ggagcgaccc
      601 ggagcgacag cggaggggca cgccctgaga attattggtg cgcagagtag ctttttacaa
      661 tcggtcgttt cgggaatgtt gaggtgggtg tgccttcgcg caaaattcgg ttatgtagtt
      721 cgttttagtt atagcatctt tcgtagatcg cgacggttga gtgggtagcc acagctcttg
      781 caaaaccttg ctccatcaag gtgcttgtcg tcatggcagt tctggcagac ctgggtgttg
      841 gtaaaagtgt ctttgaattt tgtgccacgt aaggtctctg tggcaatgat tgttggcacg
      901 aatatgactc catagcctat gagcatcaat acagaagcta agaatctgcc catggcggtc
      961 accggtgtga tgtcgccaaa gccaacggtg gtgacggtaa ctactgccca gtagatgctg
     1021 gtaggtatgc tcttcattgg cgactcgggg tggccacttt ctataacgta tatcagtgca
     1081 ccgatgacta ctaccacaag ggtgatgaat gacaagaata ctccaatctc ggacaaactc
     1141 cgcttgagtg ctgtcatgat gaggttgccg ccgcgcatga attttgttag cctgaatatc
     1201 ctggagattc gtaggagtct gaggatgcga atactggcca agaacttggt atttgggtaa
     1261 aaaattccca gatacgtagg cagaatgctt actaagtcga tgatgccata aaggctgaat
     1321 atgtatcgaa taggccatct gacgatgtaa agtctcagaa agtattcgat ggtgaagagt
     1381 atggttatgg tccactcaag gatgacaagt atgttgtggt agcgggcatt gatctcgggt
     1441 accgactcca tcatgaccag gcctacgttg atgagtataa gtgccaatag cgcaatattg
     1501 aaaacacgac cgtagtaggt gtcggtctcg aatatgattt cgaaggtttt ttctttccat
     1561 tgattttctt ccggtttttc catcgtaggg aagatcaggt cttccttaaa ttttaccttt
     1621 ttgaaccaca tgatcttata attatatgcc agctttgttt taaaatatga aaatgacgga
     1681 ttttcttgtc aaaataaata ttgtagcatg attgtgtatc aatagttatg acgtatgtgt
     1741 aggccaggaa gtatcgctct tgccccaatc cgttaccttg aacagatgca acacacccgg
     1801 gaatagggcg tccatgcttt ctttggcacc tcgggtagag cccggcagtg tgagtatcag
     1861 ggtatcgcca cgtagccccg aaacaccacg cgacaacatg gcataaggca tgcgttgctg
     1921 tccgtagctg cgtgctgcct ccatgatgcc cggtatgtgg cgttctatga gtgggcttac
     1981 tgcctccggt gttaggtctt tggcagacag gccggtaccg cctgtgatga tcactaccct
     2041 gatgcccttg tcgcaaaagg ctgtcacttt ttgttggatg gtctgtacat catcgggtat
     2101 gatggtgtag tcaagaactt gtatgttgtg ctgttctagt ttgctgatga tggctttgcc
     2161 ggcggagtcc tgttttttgc ctccggcaat actgtctgag cagaccacga ctgctgcgtc
     2221 aatgcttcta tcaaattcat ctctgaactg cgacttgccg cctttcttgc tcaggagccg
     2281 gatgttctga atctcgatgt atttgtcaat cggcttgagc atgtcgtaga tggtgagtgc
     2341 tgccaccgaa gcaccgtgca tggcttctac ttctacgccg gtgcggtaga tggtatgtac
     2401 ttctgtgctg atgatgatgc tgagtccttc aatatggtgt gagactgagg tgtattcgat
     2461 gggtagtggg tggcagtcgg gtatcacatc gctggtgcgt ttgacggcaa acaatgctgc
     2521 cacacggctt gcttcgagca cgtcgccctt gggtacggta cgattttgta cagcttcaat
     2581 ggtttcggcc ttgctaaccc ttacggtagc ctgggcaatg gctgtgcggt gggtggttat
     2641 tttgtgtgta atgtctatca tcgtgtcagg tgttttgttt ccatttggtt ttttggtctg
     2701 tcaatatttc tttgccccat acaggagctt cttttttgat gcgttctacg atttcttcac
     2761 atgccgagat ggcgtcggca cgatgggcag aagatgtaaa cacgaacagg cttatttcgc
     2821 ctgcttgtac agtgccgagg ctatgatata tgtgcataca ggtgagtgca tattttgcaa
     2881 atgtctcttc ccgaatgtgg tgaaatactt cttcggccat agcgtggtag gtagtatatt
     2941 cgatggcgcc gacttcgccg ttttcgtgtc ggtcattgcg tacttgccca aggaagatac
     3001 tgtgtgcacc gatattgagt tttttactgt gtttctcaat cgattcggca atgaaaccgg
     3061 acggtatcgg gccttcaaca aacacttgat gttttttctt ggtttccatt ctatcggtat
     3121 tgttttctta tttgttttgt aaaatatgta tttgtaccca gttgtctatg ccgcccttta
     3181 aactataggc ttgtacagat gttttatttt tttgtaaaat ttgccatgcc agcatacttc
     3241 tttttcctga cttacagaac agaacgattg gtttgccctc cggaatttct tccaggcggt
     3301 cttcgaattg ggcaagcggg atattcgtgt atggaaatcc ttcaactgtg ggctgttcat
     3361 acggttcgcg aacgtcgatg atgaacaaat cttcgttggc tctcattata tcaaaccggg
     3421 caatcggtat ttcatattcg cttagcttat cggcacaatg ccaggcatag tcccatgcac
     3481 ggaatttttc actcgtatcg ggcatctgcg aatgtttttc ttttattggt gaaatctcta
     3541 tttcgtaata cctgttattg agcacattga aagataggat tttattgcaa agtggcactc
     3601 ctgtgcccgt gatgatcttg atggcttcgg ctgcctgcat acttccggtg atgcccggta
     3661 ccacaccaag cacgcctgct tcattgcatg aggtgacggc agcaatatcg ggcggatgag
     3721 gaaaggcatc gcggtagttc gtcttgatgc ccgtctttgg atctttaaga ttgaacacag
     3781 ccacctgagc ttcgtagcgg agcaccgcgc cataaaccaa tggtttgtca agcagtacac
     3841 acgcatcatt gagcatgtat cttgtagcga agttatctgt tccgtctatg accaggtcgt
     3901 attgttctat gatgtccagg gcatttgagc tatcaagcct gatgctgtgg gcgatgtatt
     3961 tgatatcagg gttaagtgcc gacaagtgcg caatggcaca agataatttg ggcttgccga
     4021 tatcatatat attatatagt gtctgtcgct gcagattgct cagttctact acatcatctt
     4081 cggctatgcc tatacatccg acaccggctg ctgccaggta tagaagggca gggcagccaa
     4141 gtccaccggc tccggctact aatacctttg ccctggcgag cttttgctgg gctgcttcgc
     4201 caaaaccact gatggaagtc tggcgttggt accgatttgc tttgtcaatg tgtgtcatgt
     4261 tatatttatc cgcccgaaaa tggtggtagc agtgcaacag tatcgttttc attgagtaca
     4321 gttacgtctt gtgccatctt ttggttgacg gctagtttga agcttttgct ctttagttcg
     4381 gggtgttttt c
//
LOCUS       DAOQQY010000240         4390 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9324_length_4390_cov_4.736101, whole genome shotgun sequence.
ACCESSION   DAOQQY010000240 DAOQQY010000000
VERSION     DAOQQY010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9324_length_4390_cov_4.736101"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLE23_10355"
     CDS             <1..953
                     /locus_tag="PLE23_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83708.1"
                     /translation="SNLKSNFDLKENDFDIRLKATKWFSDNFSFALTTGNHLTTLTDS
                     IKSSLSNFYLRPAVTFSGDMFRILAGVNLVATNDEFKVLPDLEAMVSVAGNAFGVYAG
                     WYGDVQKNTYRSLMQQNPFIVQDVTLTNSAYNRIYGGIRGRIGAIQYQGEAGYKSISG
                     MPLFVNDSLRPYGFGLVYDDLKIFDINGTVILDLARGLQFTGLVKYNIYTTDKEKEPW
                     HLPNLESNLGLSLLTLNDKLKLKAEVYLADATYSKNYETLKPQKNNALLDLSFEAAYR
                     FSEYLGIFFQLNNLPNSKYKRYYNTPSYGLNILGGVTARF"
     gene            complement(970..2955)
                     /locus_tag="PLE23_10360"
     CDS             complement(970..2955)
                     /locus_tag="PLE23_10360"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HMX83709.1"
                     /translation="MEPNFDFGKLLWKPSEKFAASSNLNLYRIWLNEQYGLSLQDYDD
                     MHRWSVENIGSFWESITQYFDVMFHTPYDQILNEKNMPHTDWFQGATLNYAEHIFRNN
                     FADEPAIYYQSERLACSHICWNDLRSRVARFAAFLRHKGVRQGDRVVAFIPNIPEATI
                     AFLAANSIGAVWSSCSPDFGAESVVDRFSQITPVVMICADGYYYNGKTFPKWDVIKSV
                     AEKIPSLHTIVSVSFIGEKYPADMSTDVVSWDEALNNDAQLLSFTPVPFNHPIWVLYS
                     SGTTGIPKAITHSTGGMLLEHLKYLTFHNDVKPGEKFFWYSTTGWMMWNYTNAALLAG
                     ASIVLFEGNPAYPDVEVLWRMAQDTGLNHFGTSAPYIVNLMRKGFSPSAHFNLEQLRS
                     LSSTGSPLPPEAFDWVYHNIKEDLWLCSMSGGTDVCTAFVGGCPIKPVYQGEIQCIAL
                     GCDLQVYDESGRELQDEVGEMVIVQPMPCMPVYFWNDPDYMRYQESYFEMFPGIWRHG
                     DWVRITKSGELIIYGRSDATLNRHGIRIGTSEIYSAVNTVPEVKDSLVVNLELSGGEH
                     FMPLFITMNEGATFTEAVISNIKTALRSLYSARHVPDEIILTDDIPYTISGKKMEAPV
                     KKILMGIALEKAVKLDSMRNPDSVLFFIRLAKERQFR"
     gene            3059..>4390
                     /locus_tag="PLE23_10365"
     CDS             3059..>4390
                     /locus_tag="PLE23_10365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMX83710.1"
                     /translation="MKKILVANRGEIALRVMKTIKKMGISTVAVYSDIDRYAPHVRFA
                     DEAVCIGVAHSSQSYLRGDHIIEVALSLGVDGIHPGYGFLSENAGFARKVTEAGITFI
                     GPSPESIEIMGSKIGAKEAVKSYGIPMVPGMDRAIIDKDEAIKVATQVGYPVLIKASA
                     GGGGKGMRVVQNAEELPEQMERAMSEALSAFGDNSVFIEKYIASPRHIEIQVLADQHG
                     NVVHLFERECSIQRRHQKLVEEAPSAILTPEVRAAMGKSACDVARAAQYQGAGTVEFL
                     VDEHLNYYFLEMNTRLQVEHPVTEWICGLDLVEQQIRVARGEKLSFTQNDLKINGHAI
                     ELRICAENPAENFLPSTGTLNRFFLPDMENVRLDTGYTEGMEIPVQYDPLIAKLITWG
                     NTREAAMNKMVEAIDHFTIDGIQSTLEFGKFVMRHDDFISGHFDTNFIQKNF"
BASE COUNT         1252 a          955 c         1029 g         1154 t
ORIGIN      
        1 tttcgaacct caagtcaaat tttgacctga aggaaaacga ttttgacatc agactaaaag
       61 ctactaaatg gttttcggac aatttttcgt ttgcacttac taccggcaac cacctgacca
      121 ccctaaccga ctctatcaaa tcgtctctta gcaattttta tttaaggcct gctgtcactt
      181 tttcgggtga catgttcagg atacttgccg gagtaaacct tgtggccacc aacgacgagt
      241 ttaaggtatt gcctgacctc gaagcgatgg tcagtgttgc gggcaatgcc tttggtgtgt
      301 atgcaggatg gtatggagat gtacagaaaa atacctaccg aagcctgatg cagcaaaatc
      361 ctttcatcgt acaggatgtg acacttacca attcggctta taaccgcata tatggtggca
      421 taagaggccg aatcggtgcc atccagtacc agggagaggc aggctacaaa tcgataagtg
      481 gcatgccatt atttgtaaat gattcgctgc gtccgtacgg attcgggttg gtatatgacg
      541 acctgaagat atttgacatc aacggtaccg tgatactcga tctagcccgc gggttacagt
      601 ttacaggatt ggtgaaatac aatatttata caaccgacaa agaaaaagag ccttggcacc
      661 ttcctaacct ggaatcgaac cttgggttgt cacttcttac actcaatgac aaactcaagt
      721 taaaagctga ggtatatctt gcagatgcta cgtacagtaa gaattacgaa acacttaagc
      781 cacagaaaaa taatgcactg ctcgacctaa gttttgaagc tgcttacagg tttagcgagt
      841 atttgggtat ttttttccag cttaacaacc tgccaaacag caagtacaag cgatactaca
      901 acacaccaag ctacggtctg aacatactcg gtggggtaac cgccaggttt taggtatcat
      961 gaaaattcat tatctgaact gcctctcttt ggcaagacgg ataaagaata ggaccgaatc
     1021 gggatttcgc atagaatcga gcttaacagc tttttcaagt gcaatgccca tgagtatttt
     1081 tttgacagga gcttccattt tctttccgct gatggtgtaa ggaatgtcat ccgtaaggat
     1141 gatttcgtcc ggtacgtgac gagcagaata caatgaacgg agggcagtct taatgtttga
     1201 gatgacagct tcggtgaaag ttgcaccttc attcatggta atgaataatg gcataaaatg
     1261 ctctccaccg ctgagttcaa gatttacaac gagagagtct ttcacttccg gtactgtgtt
     1321 gacggcgctg tatatttcac ttgtgccgat gcgtatgcca tggcggttaa gggtagcatc
     1381 gctgcggcca tagatgatga gttcgccaga ttttgtgatt cttacccagt cgccatgccg
     1441 ccagataccc ggaaacattt cgaaataaga ttcttgatac ctcatgtagt cggggtcgtt
     1501 ccagaaataa accggcatac atggcattgg ttgtacgatg accatttctc ctacttcatc
     1561 ctgtagctcc ctacccgatt cgtcataaac ctgcaagtcg caaccaaggg cgatgcactg
     1621 aatctcacct tgatatactg gctttatagg gcatccgcca acgaaggctg tacacacatc
     1681 ggtgccgccc gacatactac acagccacag gtcttctttg atgttatgat agacccagtc
     1741 gaatgcttca ggtggcaatg gcgatccggt agaactaagg gaacgcagct gctcaagatt
     1801 aaaatgggca gaagggctaa aacctttgcg catcaggtta acgatatatg gtgcgctggt
     1861 accgaagtgg ttcaagccgg tgtcctgtgc catcctccac aatacttcca catccggata
     1921 agcaggattt ccttcaaaaa gcacaataga agcacctgcc agcaatgctg cattggtgta
     1981 gttccacatc atccagcctg tggtcgaata ccagaagaat ttttccccgg gctttacgtc
     2041 attgtggaaa gtaaggtact tgaggtgttc gagcagcata cctccggtag aatgtgtgat
     2101 ggccttcgga atgcctgtgg taccactgct atacagtacc cagatcgggt ggttgaaagg
     2161 caccggagta aaactgagta actgagcatc gttgttaagt gcttcatccc agctgaccac
     2221 atctgtgctc atgtcggccg gatatttttc accaataaaa gaaacactta caatggtatg
     2281 caatgaaggt attttttcag ctacactttt gatgacatcc catttgggga atgttttgcc
     2341 gttgtaataa tatccgtcgg cacaaatcat gactacggga gtgatttgcg agaagcggtc
     2401 aacgacactt tcggctccga aatcgggaga gcaactcgac cacactgcac ctatgctatt
     2461 agctgcaaga aaggcaatgg ttgcttcggg gatgtttgga ataaaggcaa caactctgtc
     2521 gccctgcctt actcccttgt ggcgaaggaa cgcggcaaag cgtgctaccc tactgcgcag
     2581 atcattccag cagatatgag aacaggcaag tctttctgat tggtagtaga tggcaggctc
     2641 gtcggcaaag ttatttcgga aaatgtgttc tgcatagtta agtgttgcac cttgaaacca
     2701 atcggtgtgc ggcatatttt tctcgttcag tatttggtca tagggtgtgt gaaacatcac
     2761 atcaaaatac tgggttatgc tctcccaaaa cgaaccgatg ttttcaaccg accaacggtg
     2821 catatcgtca taatcctgga ggctgaggcc gtactgttca ttcagccaga tgcggtacaa
     2881 gttcagattg cttgatgcag caaatttttc agaaggtttc cagagcagtt ttccgaagtc
     2941 gaaatttggt tccataattt ttcggtaaat agaattatgt tacaaactta tgaattcatc
     3001 cttagtgatt attaaaattc aggattttat ttcatttttg caagatacta ctcagaatat
     3061 gaagaaaata cttgtagcca acaggggcga gatagcattg cgagtgatga agacaatcaa
     3121 aaaaatgggt atcagcacgg ttgccgttta ttctgatatt gaccggtatg ctccgcatgt
     3181 aaggtttgcg gatgaagctg tctgtatagg tgtggcacat tcgtcgcagt catatctgag
     3241 gggcgatcat attattgaag tagcgttgtc acttggtgtt gacggcattc atccgggata
     3301 tggttttttg agtgaaaatg ccgggtttgc aagaaaagtt accgaagccg gtattacatt
     3361 catcgggcca tcacctgaat caatagagat aatgggcagt aaaatcggag ccaaggaagc
     3421 tgtaaaatca tacggcatac caatggtgcc cggaatggac cgcgccatca tagacaagga
     3481 tgaagccata aaggtagcca cacaggtagg ttatccggta ttgataaagg catcggcagg
     3541 tggtggcgga aaaggtatgc gtgtagtaca aaatgccgaa gaactccccg aacaaatgga
     3601 acgtgccatg agcgaagctc tttcagcctt tggagacaat tctgttttta tagaaaaata
     3661 catcgcttcg ccaagacaca tagaaataca ggtactggct gatcaacacg gcaatgtagt
     3721 tcatcttttt gaacgcgagt gcagcatcca gcgaaggcat cagaaactgg tagaagaagc
     3781 accttcggca atactgacac ctgaagtacg agctgctatg ggaaaatcgg cctgtgatgt
     3841 agcaagagct gctcaatatc aaggtgcagg tacggtagaa ttcctcgttg atgaacacct
     3901 caattactac ttcctcgaaa tgaacactag gctgcaggta gaacacccgg taacggaatg
     3961 gatctgcggg cttgacctcg ttgagcaaca gatacgtgtg gcacgaggcg aaaaactcag
     4021 ctttactcag aacgatctaa agataaacgg ccatgcaata gaactcagga tatgtgcaga
     4081 aaacccggct gagaatttcc ttccttcaac cggtacgctc aataggtttt ttctccctga
     4141 catggaaaat gtaaggctcg acacaggcta caccgaaggc atggaaatac ccgtacaata
     4201 cgacccatta atagccaaac tcattacgtg gggcaataca agagaagctg ctatgaacaa
     4261 gatggttgaa gctatcgacc attttaccat tgacggcatt caatctacgc ttgagtttgg
     4321 gaaatttgtc atgcgtcatg atgattttat ctcgggccac ttcgacacca attttataca
     4381 aaaaaatttc
//
LOCUS       DAOQQY010000241         4346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9475_length_4346_cov_6.393615, whole genome shotgun sequence.
ACCESSION   DAOQQY010000241 DAOQQY010000000
VERSION     DAOQQY010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9475_length_4346_cov_6.393615"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="PLE23_10370"
     CDS             complement(<1..853)
                     /locus_tag="PLE23_10370"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HMX83711.1"
                     /translation="MKLLNKLPVTMVTIMLLLTLKTIAQNSGSLVPLPVSLTEKKGNS
                     TLSGSVSIFCNEPSLFDALQLFSSQLALTSGIKSGKTANQQQADIIILKQQDYKSEQY
                     SLNISPDKIVIKAATPQGVFYAGQTLMQLCPPEIFGNKKSIMGKIELPCMEIKDYPRF
                     QYRGMHLDVCRHFFPKEFVMKYIDFIAMHKMNYFHFHLTDDQGWRIEIKKYPKLTEIG
                     SKRKGTLIGSYSDVPHRFDTIAYGGYYTQDDIRQIVDYAAKRYVTVIPEIEMPGHAMA
                     AIAAYPEL"
     gene            912..1301
                     /locus_tag="PLE23_10375"
     CDS             912..1301
                     /locus_tag="PLE23_10375"
                     /inference="COORDINATES: protein motif:HMM:NF017935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HMX83712.1"
                     /translation="MAKPGVIGFLISTFGLSCPRCRKADLFYYPVLRFKKLYKMKSEC
                     DYCGQDFEIEPGFYYGSMFISYIISSFFMLISFGIIKFAFKLDVWTTFGIVMALLVLV
                     YFYVFRISRSLWIHFFVKYNPKAVKKQ"
     gene            1460..2383
                     /locus_tag="PLE23_10380"
     CDS             1460..2383
                     /locus_tag="PLE23_10380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMX83713.1"
                     /translation="MLKTYFSFILLIFVCTFVRAQEAPMYSHFLFNKLAYNPAFAGGK
                     DVAVVQALYRQQWQNIVGAPRTANVNFHTPFAQQKCGLGMMVTQDQLGLLSNTYADLM
                     YAYRLKTSETGVLSVGLQARIEYARFNWNKAKSISPTDNNLQNENENLTKPNFGVGAY
                     YKDKNFFFGFSVPQLLESTLYRSSYEDLPSLHRFRTYYLMTGAELALSPSVDFMPGVL
                     VSYIANAPLQAEINANLVFSKTILVGASYRVGSSVDGLVGIQLTKQLMLGLAYDYSIS
                     KLRKENIGSFEAMLEYQFDYNRTGVNHLRYF"
     gene            2409..>4346
                     /locus_tag="PLE23_10385"
     CDS             2409..>4346
                     /locus_tag="PLE23_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMX83714.1"
                     /translation="MARFIYFSLSFIFFLLVTLTVNAQTADVKDQIPDIRAEKYYSIE
                     GYQKAIDIYEKEDPAKMSRETQLKVANAYRLNGNSVMAEKYYALCINESSKPQDVYNY
                     AQVLLSNGKCHESIPYFNTYMRSAGEKSMNNVISSCDELERFPVNNYVDITIAEGLNS
                     KWLDFAPVFYKNGLIFTSGRPNNLNTKNKDYWSGKPFMDVYYAEAQGNGKFETPGLLS
                     KTLSTKYHDGAATFTPDGNEMYYSSNNRSGKSKKGIKDLKIYTAKLINGSWTNSDAFP
                     FNSDDYDNCHPALSADGNILVFASDMPGGQGGMDLYVSFRKKNIWSRPVNLGKQINTP
                     GNELFPFIDARGTLYFSSDGQKGMGGLDIFSAAMEKSETIWSTPVNMGRPFNSLKDDL
                     SFIVSTSGLDGYFASNREGGMGEDDIYYWTNKPDYRPNQGLLAIKVLDTETNMPVGDA
                     RVNLFDEHSQSTGYLTNQDGLIGKDLSRRDVKFIEYKKDGYETGLKYLSDDMPSKPQY
                     TIIYMKPEKGQLISGLVTDKSTGRPLPGAEVFVSGMPNGPFKSVITAPDGTYSIKVPC
                     VGEYKLAATKQDYVKSERNLMADEIPCNVSNAVVRDFALSPYMMNDKRRELSASFLGD
                     KDAMFEAGKTFEVKDIYYDYNK"
BASE COUNT         1258 a          856 c          905 g         1327 t
ORIGIN      
        1 atagctcagg gtaagctgct attgctgcca tagcgtgacc gggcatctct atttcgggaa
       61 tcacggttac atatcttttt gctgcataat caactatctg gcgtatatca tcctgggtgt
      121 aatacccacc gtatgcgata gtatcaaacc ggtgaggaac atcgctgtaa ctgcctataa
      181 gtgttccttt gcgcttgctc cctatctctg tcagtttcgg atatttcttt atctcaatac
      241 gccatccttg atcgtcagtt aggtgaaaat gaaaataatt catcttgtgc atggcgataa
      301 agtctatgta tttcatcacg aattcctttg gaaaaaaatg cctgcaaacg tcgaggtgca
      361 tacctctgta ctgaaatctt gggtaatcct tgatctccat acaaggtaat tctatttttc
      421 ccatgattga ttttttgtta ccgaatattt caggggggca gagttgcatg agtgtttgcc
      481 cggcatagaa tacaccttgt ggtgtggctg cttttatcac tattttatcc gggcttatgt
      541 tcaggctgta ttgctcagat ttatagtctt gttgttttaa tatgatgatg tcggcctgct
      601 gctgattggc tgttttgccc gatttgattc ctgatgtgag cgctaactgg ctactgaaca
      661 gttgcaatgc atcaaataag gatggttcat tgcaaaaaat gctgacgctg ccgctcaatg
      721 ttgagtttcc ttttttttca gtcagtgaaa caggcaatgg taccaacgat ccactgtttt
      781 gagctatggt tttcaaggtc agtaacagca ttattgtaac cattgttaca ggcaatttat
      841 tcagtaattt cataactttg cagcttattt atcagcaaag ataacccaaa caattttaaa
      901 acaggatatt catggcaaaa cccggtgtta taggtttttt aatcagcact tttggactca
      961 gctgtccgcg atgcagaaaa gcggatttgt tttactatcc ggtattgagg ttcaagaaac
     1021 tatataaaat gaagtctgaa tgcgattatt gcggacaaga ttttgaaata gaaccgggct
     1081 tttattacgg ttctatgttt atttcttata tcatcagttc atttttcatg cttatcagct
     1141 ttggcattat caagtttgcg tttaaacttg atgtttggac tacattcggc atagtcatgg
     1201 cattgcttgt gcttgtttat ttctacgtgt tcagaatatc aaggtcattg tggatccatt
     1261 tttttgtaaa atacaaccca aaggcggtaa aaaagcaata atgtgctgac agacaaaagt
     1321 aacagccttt atattttttt aatttattat tattcaaatg tgtagaaatg gttcggtttt
     1381 tgataaaata gtgttataga gaattcattc gcacgaatga tacctatgtt attattagaa
     1441 aaccaatgac actattgcta tgctgaagac ttacttttcc ttcatactat tgatttttgt
     1501 ctgcactttt gtccgggcac aggaggcacc gatgtattca cattttttat ttaacaaact
     1561 ggcttacaac ccggcttttg caggtggaaa agatgtggca gttgtacagg cattgtacag
     1621 gcaacaatgg cagaacatag taggcgcacc caggactgca aatgttaatt ttcatacgcc
     1681 ttttgctcag cagaaatgtg gtctgggtat gatggtgaca caggaccagc tcggcctgct
     1741 aagcaatacc tatgccgacc ttatgtatgc atatcgtctg aaaaccagcg aaacaggtgt
     1801 actgagtgta ggtcttcagg ccagaataga atacgcacgc ttcaactgga acaaagctaa
     1861 atccattagc ccaactgata ataacctgca aaatgaaaac gaaaatttga ccaaacctaa
     1921 ctttggcgtc ggagcgtatt ataaagataa aaatttcttc tttggctttt cggttcctca
     1981 attacttgaa tcgaccttgt acagaagttc gtatgaagat ttgccttcac tacaccggtt
     2041 ccggacctat tatctgatga caggtgcaga attggctctt tcaccatcgg ttgactttat
     2101 gccgggtgta ttggtcagct atatagctaa tgccccactt caggctgaga taaatgccaa
     2161 tctggtgttt tccaaaacta tccttgttgg tgcatcctac cgtgtaggtt cttcggttga
     2221 cggactcgtt ggcatccaac ttacaaaaca gcttatgttg ggcttggctt acgattattc
     2281 gatttccaaa ctacgcaagg aaaacatagg gtcatttgaa gcaatgctcg aatatcagtt
     2341 cgattacaat cgcactggag tcaatcacct gagatatttt tagttttaac caacctgaaa
     2401 ttttacccat ggccagattt atctattttt cattgtcatt tatatttttt cttttagtta
     2461 ccttgacggt gaatgctcag acagcagatg ttaaagacca gattcctgac atcagagctg
     2521 agaaatatta cagcatcgaa ggctaccaaa aggctataga tatatatgaa aaggaagacc
     2581 ctgcaaaaat gagcagggag acacaattga aagtcgctaa tgcctacagg ctaaatggca
     2641 attctgttat ggcagaaaaa tattatgccc tctgtatcaa tgaaagttct aagccgcagg
     2701 atgtttataa ttatgcccag gttttattgt ccaacggcaa atgccatgag tcgattccgt
     2761 atttcaatac atatatgcga tcagccggcg aaaaaagcat gaataatgtc atcagttcgt
     2821 gtgatgaatt ggagcgtttt ccggtcaata attatgttga tattaccatt gctgaaggtc
     2881 tcaactccaa atggcttgat ttcgctccgg tattttacaa aaatggtctg atttttactt
     2941 cgggcaggcc aaataattta aatacaaaga acaaagacta ctggagtggc aaacctttta
     3001 tggatgtgta ctatgctgaa gcccaaggca atggtaaatt tgaaacaccg ggcttgttgt
     3061 caaaaacact cagtacaaag taccatgacg gtgccgctac atttactccc gacggcaacg
     3121 agatgtacta ttcttctaac aaccgctccg gaaagagtaa aaaaggtatc aaagacctga
     3181 agatatatac tgccaagctc ataaacggat catggaccaa cagtgatgca ttcccattta
     3241 atagtgatga ctatgataat tgtcatcctg ctctttctgc tgatggcaat atcttagttt
     3301 ttgcttctga tatgccgggt ggccaaggtg gtatggacct ctatgtgagt tttagaaaga
     3361 agaatatttg gtcaaggcct gtcaaccttg gcaaacagat aaatactccg ggcaatgaac
     3421 tgtttccatt tattgacgcc agaggtacgc tctatttttc atctgacgga caaaaaggta
     3481 tgggcgggct cgacattttt tctgctgcca tggaaaaatc tgaaaccatc tggtccacgc
     3541 cggtcaatat gggcagaccc tttaattcat tgaaagacga tctgagcttc atagtatcca
     3601 cctctggtct tgatggatat tttgcttcta accgcgaagg cggaatgggg gaggatgaca
     3661 tatattactg gacaaacaag cccgactatc gccccaatca aggtctgctg gctataaaag
     3721 tactcgacac agaaaccaat atgcctgtag gcgatgccag ggttaatctg ttcgatgagc
     3781 actcacaaag taccggctac ctgaccaatc aggatggttt aataggcaag gatctcagcc
     3841 gtagggatgt taaatttatt gaatacaaaa aggatggtta tgaaacaggc ctcaaatatc
     3901 tgtcggatga tatgccttca aaaccacaat acactataat atatatgaaa cccgaaaaag
     3961 gccagcttat ttcaggcctg gttacggata aatctacagg gcgaccactg ccgggtgctg
     4021 aagttttcgt ttcaggaatg cctaacggac cattcaagtc tgtgataact gcccccgatg
     4081 gtacatattc cataaaagta ccttgtgtgg gagaatacaa actcgctgct accaaacagg
     4141 attatgtaaa gtcagaaaga aatttgatgg cggatgaaat accctgcaat gtatccaatg
     4201 ctgtggtacg tgacttcgct ttgtctcctt atatgatgaa tgataagcga cgcgaactca
     4261 gtgcttcgtt cctcggtgac aaagatgcta tgtttgaagc cggtaaaaca ttcgaagtaa
     4321 aagacattta ttatgattat aataag
//
LOCUS       DAOQQY010000242         4341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9492_length_4341_cov_4.587028, whole genome shotgun sequence.
ACCESSION   DAOQQY010000242 DAOQQY010000000
VERSION     DAOQQY010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9492_length_4341_cov_4.587028"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="PLE23_10390"
     CDS             <1..311
                     /locus_tag="PLE23_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HMX83715.1"
                     /translation="GENYTGWTSIGCRIRGIKDGKEKTYYVWNNCSHEVAYKETGTQG
                     VSYTTGVPATIGAMMMLTGEWKGEGVFNVEQFNPDPFMALLNTEGLPWKEAHDIDLEL
                     "
     gene            315..1457
                     /gene="nspC"
                     /locus_tag="PLE23_10395"
     CDS             315..1457
                     /gene="nspC"
                     /locus_tag="PLE23_10395"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HMX83716.1"
                     /translation="MSIPYHLINPPAFVLDESALRRNLELINRIQKEAGVSFILAFKG
                     FSMWSAFPIVREYVKGATASSLHEALLCYEEMGVKAHTYAPAYIPAEFHRIMELSSHI
                     SFNSISQYERFREQVRNYPQHISCGIRVNPEWSDVETDLYNPASPTSRLGELYENFNG
                     ILPEGIEGLHFHVLCESTSFALETVLENLEKRFGNFLKQIKWLNMGGGHLVTKSSYNV
                     EHLIQVLKKFKQKYPQLDIILEPGSAFAWETGDLVTSVLDIVDHRGVKTVIIDASFTA
                     HMPDTLEMPYKPKIEGARDPHEGEKGVRIGGSSCLAGDYKGDYVFEKELKPGELIVFK
                     DMIHYTMVKTTTFNGVKHPDICIYRMDGIIEVVKMFGYLDFKNRLS"
     gene            1460..2299
                     /gene="prmC"
                     /locus_tag="PLE23_10400"
     CDS             1460..2299
                     /gene="prmC"
                     /locus_tag="PLE23_10400"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMX83717.1"
                     /translation="MTFAEAFSDSIELLAATFDRREAENIVRILGEDFFQVYSLHSQK
                     NISDDQYALLCSASEKILEGIPLQYLTNRADFMGLEFYVNENVLIPRPETEELTDWIM
                     KTFPKDLPVRILDIGTGSGCIPISLSKYAPAWKLDAIDISAPALEVAEENARQHHVKV
                     NFTQKDILTASADDFNHKYDAIVSNPPYIGFDELDKMDNHVLKSEPRIALFAGEDPLV
                     FYRKIASCAKDLLKTNGCLFFELNPLYAEEIKEICRNVGFTLVEVKKDMQGNLRMIKC
                     CNS"
     gene            complement(2302..2661)
                     /locus_tag="PLE23_10405"
     CDS             complement(2302..2661)
                     /locus_tag="PLE23_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HMX83718.1"
                     /translation="MKKNKNSDRDRIVYSTDLGSLRNFFDTTEDETDSVTPKSKQVVR
                     VTLDRKQRGGKEVTLVTGLEENDETIKELGSFLKSKCGVGGSAKNGEIIVQGDHREKV
                     IQLLKEKGYTNTKRTGG"
     gene            complement(2658..3539)
                     /locus_tag="PLE23_10410"
     CDS             complement(2658..3539)
                     /locus_tag="PLE23_10410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HMX83719.1"
                     /translation="MQKQTIACIINPKSGVRQNKNLQDEILSTLDTDRYHIEFFETKR
                     RGHATELAEKIVSEGYDILLVCGGDGTINEAVQPIVNKDILLGIIPTGSGNGLSMHTG
                     IGREHTEAIHIINRQKILKIDCIRYGDKFLINLAGIGFDGFLTKIIARKTYRGFWLYI
                     WLFIRYFWTYKPSTYYIETNTESIKGRYNMIEIANGPMYGYNFEVVPGADIADGIIDI
                     LLLKKTPIWRILLDSVKLMKNKWEQISWAKRIQCTSVSIQSDAPLKMHVDGEYVDQNV
                     TSIAFQILPKSINFIIP"
     gene            complement(3595..>4341)
                     /locus_tag="PLE23_10415"
     CDS             complement(3595..>4341)
                     /locus_tag="PLE23_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HMX83720.1"
                     /translation="VVALGAAIQADVLAGNQRDVLLLDVTPLSLGIETVGGLIDVLIP
                     RNTKIPCKAGRQYTTSIDGQVNLKIAVYQGERELVADNRKLGEFILKNIPPMPAGLPK
                     IEIIFTLNSDGILKVKAKELRSDTSQEIEIRPTYGISEEEMSLMLLDSIKNAKEDMQT
                     KALLEAVTEAQTNLLATQKFLKQNDSWLSNAEKQTIMELYNRLEEATKGNDKDIIYSI
                     MQELNDYSEPLAHKAMDYSIAQSLKGTKIE"
BASE COUNT         1186 a          866 c          938 g         1351 t
ORIGIN      
        1 tgggtgaaaa ctacaccgga tggacaagca taggctgcag aattagaggt atcaaagatg
       61 gtaaagagaa aacctactac gtgtggaata attgtagcca cgaagtagca tacaaagaaa
      121 ccggtacaca aggcgtatca tacaccactg gcgtacctgc taccatcgga gccatgatga
      181 tgctcacagg cgaatggaaa ggcgagggcg tattcaatgt cgaacagttt aatcctgatc
      241 catttatggc cctgctcaat acagaaggcc tgccatggaa ggaagctcat gacatagacc
      301 ttgagctgta aaatatgagt atcccgtacc accttatcaa ccctccggct tttgtgctgg
      361 acgaaagtgc attgcgccgc aatctggagc ttattaaccg tattcagaaa gaagcaggtg
      421 ttagttttat cctggcattt aaaggctttt cgatgtggag tgctttccct attgtacgcg
      481 aatacgtcaa aggtgctact gcaagttcgc tacatgaggc tttgttgtgt tatgaagaaa
      541 tgggcgttaa ggcacatacg tatgctccgg cctatatccc tgctgagttt caccgtatca
      601 tggagctgag cagccacatc agctttaatt caatttctca gtacgaacga ttccgcgagc
      661 aggtcaggaa ttacccacag cacatttcgt gtggcatacg tgtcaatccc gaatggtcag
      721 atgttgaaac cgacctatat aatccggcgt caccaacgag caggcttggc gaattgtacg
      781 agaacttcaa cggaatattg cctgagggaa ttgaaggact tcactttcat gtactttgtg
      841 agtccacatc tttcgcactc gaaacagtac tcgaaaatct cgagaaacgt tttggcaatt
      901 ttctgaaaca aataaaatgg ctcaacatgg gtggcggaca tcttgtaacc aagagtagct
      961 ataatgtaga gcatctgata caggtactta agaaatttaa gcaaaaatat cctcagctcg
     1021 acatcatttt ggaacccggc agcgcatttg cttgggaaac gggcgatctg gtcactagtg
     1081 tactggatat tgttgatcat cgtggcgtga aaaccgtcat catagatgca tcattcacag
     1141 ctcacatgcc agataccctt gagatgccct acaaaccgaa gatagaaggt gcccgcgacc
     1201 cacatgaagg agaaaaaggc gtacgcatcg gtgggtcaag ttgtcttgca ggtgattata
     1261 aaggcgatta tgtttttgaa aaagaattaa agccgggtga gcttatcgtg ttcaaagaca
     1321 tgatacatta tacaatggtg aagacgacta ctttcaatgg agtcaaacat ccggatatat
     1381 gcatttaccg tatggatgga atcatcgaag tagtgaagat gtttggctat ctcgatttca
     1441 agaacagact ttcgtagcca tgacttttgc tgaagcattt tctgattcta ttgagttgct
     1501 tgctgcaaca tttgacaggc gcgaggcaga gaatattgtc aggattctcg gcgaagactt
     1561 ctttcaagtg tatagtctgc acagccaaaa aaatatatct gatgaccagt acgcattact
     1621 gtgttcagct tcagagaaga ttctggaagg tatcccttta caatatctga ccaacagggc
     1681 tgactttatg gggctggagt tttatgtaaa tgaaaatgtg ctgatacctc gccctgagac
     1741 cgaagagctg actgattgga ttatgaagac ttttccaaaa gatttgcccg taaggattct
     1801 ggatattgga accgggagtg gttgtattcc gatatcactt tctaaatatg ctcctgcatg
     1861 gaagctcgat gccattgata tttctgctcc tgcactggaa gtagcggaag aaaatgcaag
     1921 gcagcaccat gtaaaggtaa attttaccca aaaagatatt ttgacagcct cagccgatga
     1981 ttttaatcac aagtatgatg caatcgtgtc gaaccctcca tatatcggct ttgatgaatt
     2041 agataaaatg gacaatcatg ttctgaagtc cgaaccgcgg atagctttgt ttgccggtga
     2101 agatccactg gttttttaca ggaaaattgc ttcttgtgcg aaagatttgc taaaaacaaa
     2161 cggttgtttg ttctttgagt taaatccact atatgcggaa gagattaaag aaatatgccg
     2221 caatgtgggt tttactttag tagaagtaaa aaaggatatg caaggtaatt tgagaatgat
     2281 aaaatgctgt aattcttaaa attaaccacc tgttctttta gtattggtat aacctttttc
     2341 tttgagtagt tgaatcactt tttcgcgatg atctccttgt acaatgattt ctccattttt
     2401 agcagagcca cctacgccac atttcgattt caggaatgag ccgagttctt tgatagtttc
     2461 gtcattctct tcaaggcctg taacaagggt tacttccttg cctcctcgct gttttcggtc
     2521 gagtgttacc cttacgacct gtttgctttt aggtgttaca gaatcggttt catcttcagt
     2581 tgtgtcaaaa aaatttctga gcgaaccaag gtcggtcgaa tatactattc tgtcgcgatc
     2641 cgaattttta tttttcttca tggaatgata aagttaatag attttggtaa tatttggaaa
     2701 gctatactgg ttacgttttg atctacatat tctccatcaa catgcatttt taagggagca
     2761 tcagactgaa ttgaaaccga tgtacattga atacgtttag cccaagaaat ctgctcccac
     2821 ttgttcttca tcaacttaac tgagtcgagc aatattcgcc agatcggagt tttcttcagt
     2881 aataggatat cgatgatgcc gtcggcaatg tcagcacccg gcactacttc aaaattatat
     2941 ccgtacattg gtccgttggc tatttctatc atgttataac gacctttgat actttcagta
     3001 ttggtctcga tgtagtatgt tgaaggcttg tatgtccaaa aataccgaat gaagagccag
     3061 atatacaacc agaatccacg atatgttttt ctggcaatta ttttcgtaag gaagccgtcg
     3121 aaccctattc ctgccagatt aatcagaaat ttgtcgccat accttataca atcaatcttt
     3181 agtatctttt gtctgttgat gatatgtatt gcttctgtat gttctctacc aatcccagtg
     3241 tgcatcgata atccattgcc agatccggta gggatgatgc ctagtagtat atctttgttg
     3301 acaatgggtt gtacggcttc atttatagtg ccgtcaccac cacacacaag tagaatgtca
     3361 tatccttcac ttactatctt ttctgcaagt tcagtagcgt gtccacgtct ttttgtttca
     3421 aaaaactcaa tgtgatatcg gtcagtatca agggtggaca atatttcatc ttgcagattt
     3481 ttgttctgcc tgacacccga ttttggattg atgatacaag ctattgtttg cttttgcaag
     3541 gcgctgattt ttatgtataa ttaagcagaa gataacgaat ggacatgtat ttgcttactc
     3601 aatttttgtt cctttaagag attgtgcaat agagtaatcc attgctttgt gtgccagcgg
     3661 ttcagaataa tcatttagtt cttgcatgat actatagatt atatctttat catttccttt
     3721 ggttgcctct tcaagtctgt tgtacagctc cattatggtt tgtttctcgg cattgctcag
     3781 ccaggagtcg ttttgtttaa gaaatttttg cgtagcaagg aggttagttt gggcctctgt
     3841 tactgcttca agtaaagctt tggtttgcat gtcttcctta gcattcttaa tagaatcgag
     3901 caacatcagg gacatttctt cttcgctgat accgtatgta ggccttattt ctatttcctg
     3961 gctcgtatcc gaacgcagtt cttttgcttt gactttgaga ataccatccg aattgagcgt
     4021 gaagattatt tctatcttag gtaagcctgc cggcattggt ggaatgtttt ttaggataaa
     4081 ttctccaagc tttctgttgt cggccaccag ttctcgctca ccttgataaa cggctatctt
     4141 gaggttgacc tgtccgtcaa tggaggttgt atattggcgt cctgctttgc atggaatttt
     4201 tgtatttcta ggaatgagta cgtcaatgag tccgccaact gtttcaatac caagtgacaa
     4261 tggtgttacg tcgagtaaca atacatctcg ctgattgccg gcgagcacat ctgcctgtat
     4321 ggctgcacca agagcgacta c
//
LOCUS       DAOQQY010000243         4313 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9568_length_4313_cov_5.705730, whole genome shotgun sequence.
ACCESSION   DAOQQY010000243 DAOQQY010000000
VERSION     DAOQQY010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9568_length_4313_cov_5.705730"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2217)
                     /locus_tag="PLE23_10420"
     CDS             complement(<1..2217)
                     /locus_tag="PLE23_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMX83721.1"
                     /translation="MNTKQEPKTGATKNIAYLMNPKNWWGPLLFILIISLAGVGMIGY
                     QTYVDAPPMSGFKDDAGKIIIDKQTIIRGQEVFHKYALMEYGSFFGDGAQRGPDFTAE
                     ALHYTGVFMSDYYINQFKQTKGIDPTVGDIKQINEQVKTELKKNNYDKADDIVKLTPS
                     QVFAIDKLKTFYQKKFSNEEKANGLLPSKYISSEAEINDLAAFFFWGGWVCVTERPGS
                     TFSYTHNWPFDPSAGNTPTSPVILWSVLGLLGFVLACGIVLYFIGQYNQLSNKFFKPA
                     TRDLFNTARVAAFNPTPSQKATYKFFAVAILLFFLQVSSGLITLNDFINWLGVIGIHV
                     KDNMPVTISRSWHLMLSLYWISICWIASSIFILPILSKKEIRGQVKLINTIFIMLLVL
                     AGGSLTGMILGPLGLLGEWWNLLGHQGWEFVDFGKLYQVLLMAIFAVWMVVVYRGLKP
                     AFIKGQPWNLPNWIMYSVVGIPVLFISGFVATPETNFVIADFWRWMVIHMWVEAFFEV
                     FITVIVSYLMVLMGLVSRQAAIRVVYFATIMFLGTGLLGISHNFYWNAKPVATMALGS
                     VFSTLQFVPLILLTVEAWRFKNTPKIAVGDVDHKNIGDFGFPTVFKFLVAVNFWNFFG
                     AGVLGIIINLPIMNYFEHGTYLTVNHAHAALMGVYGNISLAALIFASRLMIKNKNWND
                     KMVNISFWSINIGLMLMVLLDLFPAGSIQFKAVVEQGLWFGRSSEFVDSGAFHQLTW"
     gene            2693..>4313
                     /locus_tag="PLE23_10425"
     CDS             2693..>4313
                     /locus_tag="PLE23_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HMX83722.1"
                     /translation="MALRKNQNTGKEDEAKVRFSKESFRKSVRIFKFLKPYKWLITIG
                     LILLAVSSVVFMIFPAASGEFLNIATGKSEYNLTIRDLGIFLLVVLIIQSVVSYIRVI
                     IFTHVSEHAMADIRKTLYSKIISLPVTFFENNRVGELTSRTTNDVQQLQDALSIYVAE
                     FFRQIITLVIGIYFLVYKTPKLSLLMFATFPVIVIIAIVFGRYIRNFSKRRQEELAST
                     NVILDESLQSIAAVKAFTNEWFEVKRYGSSIDKVVKLSMSYGIWRGAFLAFVIAIVFG
                     VIFFILWQGAALVQNGTMNPGDLVSFIVVTGIIGGSIGGLGDLYTQILKAIGASERIL
                     EIMAMDSEIKIGQTSVKPIPVKGNIRFEHVRFAYPGRPEAEVLKDVSFEINSGEKIAL
                     VGSSGAGKSTIVQLIMRFYDINQGRILVDGKRIEEYDISGYRSNLAIVPQEIMLFGGT
                     IRENILYGRPSANEDEVREAAERSFAMEFIKKFPEGLDTIVGERGIKLSGGQRQRIAI
                     ARAILKDPAILILDEATSSLDAESEKLVQAAL"
BASE COUNT         1279 a          856 c          887 g         1291 t
ORIGIN      
        1 ccatgtaagt tggtggaatg cgccactatc aacaaattcg ctcgacctgc caaaccacaa
       61 gccttgctct actactgcct taaactgtat cgaacctgca gggaaaagat caagtaaaac
      121 catgagcata agaccgatgt tgattgacca aaatgatata tttaccatct tgtcgttcca
      181 atttttgttt tttatcatca gacgcgatgc gaatatcagg gcggccagac tgatattgcc
      241 atatacgccc atcaatgcag cgtgtgcatg gtttacagtc agataagtgc catgctcaaa
      301 ataattcatg atgggtagat tgatgatgat acccaatacc cctgcaccaa agaagttcca
      361 gaagttgaca gctaccaaaa acttgaatac tgttggaaat ccaaaatcgc ctatattttt
      421 atgatctaca tcacctactg ctatcttcgg tgtgtttttg aagcgccatg cttctaccgt
      481 taacagtatg agtggtacga actgcaaggt tgagaaaaca ctacccaatg ccatagtggc
      541 aactggtttt gcgttccaat aaaaattatg cgaaatgcct agaagacctg tacctaagaa
      601 cataatggtg gcaaaatata ccacccgtat ggcagcctgc ctgctcacca accccatcaa
      661 taccatcaga taactgacaa taaccgtaat gaatacttca aagaaagcct ctacccacat
      721 gtgtataacc atccatctcc agaagtcagc tatgacaaaa ttggtttcgg gtgtagctac
      781 aaacccggat ataaataata ccggaatgcc tactaccgag tacataatcc agtttggtag
      841 attccatggt tggcctttga tgaaggctgg ttttaggcca cggtaaacta ctaccatcca
      901 tactgcgaaa attgccatca gtagtacctg gtacaatttt ccgaaatcaa caaattccca
      961 gccttgatgg cctagtagat tccaccattc tcccaagaga cctaaaggtc ctagaatcat
     1021 gcccgtaagc gaaccgcctg ccagtaccaa caacatgatg aatatggtat tgataagctt
     1081 tacttgtccg cgtatttcct tttttgaaag aataggcaga ataaaaatgg aggatgcaat
     1141 ccaacagata gatatccagt acaatgacaa catcaggtgc cagcttctcg atatagtcac
     1201 aggcatattg tcctttacat gaatgccgat gacaccaagc cagttgataa agtcatttag
     1261 tgtaataagg ccacttgaca cttgaaggaa aaataatagg atagctaccg caaaaaactt
     1321 gtaagtagcc ttctggctcg gtgttgggtt gaatgcagca acgcgagcag tgttgaacag
     1381 gtcgcgtgta gccggcttga agaatttgtt tgataactga ttgtactgac caatgaaata
     1441 aaggacaata ccacatgcca gaacaaaacc aagtaagcca agtacactcc ataatatgac
     1501 aggagaggta ggcgtattgc ctgctgaagg gtcaaatggc cagttgtgtg tgtagctgaa
     1561 tgttgaaccc ggacgctctg taacacatac ccagccaccc cagaagaaaa atgctgcaag
     1621 atcatttatt tctgcttcgc tcgatatata tttactcggg agcaagccat ttgctttttc
     1681 ttcatttgaa aatttcttct gataaaaagt ttttaactta tcaattgcaa acacttgaga
     1741 tggtgtaagt ttgacgatat catctgcttt gtcataattg tttttcttca gttcggtttt
     1801 tacttgttcg tttatctgct ttatatcgcc tacggtaggg tcaatgccct ttgtttgttt
     1861 aaattgattg atataataat cactcatgaa tacaccagta taatgtaagg cttcagcggt
     1921 gaaatcgggc ccacgttgtg ctccgtctcc aaagaaactg ccgtattcca taagtgcata
     1981 tttgtggaat acctcctggc cacggataat tgtctgtttg tcaatgatga ttttccccgc
     2041 atcgtccttg aatcctgaca tgggtggagc atctacatag gtctgatagc caatcatgcc
     2101 tacacctgca aggctgatga tgagaatgaa gagaagcggg ccccaccagt tttttggatt
     2161 catcaggtat gcaatattct tggttgcacc tgttttgggt tcttgtttcg tgttcatgag
     2221 aatgtttttt agaggtttat tagatagtgt ttacatataa ttttacccaa gcgaactatg
     2281 cgtgaaaaca taataaggac aaaattgtct tttattatga attttgtcaa tacatataaa
     2341 taaatattat cattcttatg gtttgatagt cattgaatat ggtttacttt aagcattatt
     2401 tgaatggatt tttatgatgt tatgtaaata aaatatggat tactacttcc tttttatcag
     2461 atgtttaaat atggtttatc tattttattt gttaaattta aatattccta ttcttgaaaa
     2521 atgagcatat ttttgctatt ttctcagaat atttcaacaa aaactttaag taaattctaa
     2581 tacatgaata aagcttcaag tcttaaggtt tggttaaggt ttgcaattac catcatcaag
     2641 tattagccgg aaagtatgat atatgtgtaa atttgcagac agaaaattta ttatggcact
     2701 gcgcaaaaat caaaacacag gaaaggaaga tgaagctaaa gtcagatttt caaaagaatc
     2761 tttccgcaaa tctgtccgga ttttcaaatt cctgaaacct tacaaatggc tgattactat
     2821 aggcctaata ttgttggctg tctctagtgt tgtatttatg atatttccgg ccgcttcggg
     2881 cgaatttctc aatatcgcca ctggcaaatc ggaatacaac ctgactattc gcgacctcgg
     2941 catctttctg ctggtcgtac ttattattca aagtgtagta tcatacattc gtgtcatcat
     3001 cttcacgcac gtaagcgaac atgccatggc cgatataagg aaaacacttt attccaaaat
     3061 tatttcgctg cctgtcactt tctttgaaaa caaccgggta ggcgagctta ccagtcgaac
     3121 taccaatgat gttcagcagc tacaagatgc attaagcata tatgtcgcag agtttttcag
     3181 acagattatt actttggtca tcggcatata ttttctggta tataaaacac cgaagttgtc
     3241 gttgcttatg tttgccacct ttccggttat cgttatcata gcaatcgtat tcgggcgtta
     3301 catccgtaat ttttcaaaga gacgtcagga ggaacttgcc tctaccaacg tcattcttga
     3361 cgaatcgcta cagtcgatag cagccgttaa ggcatttaca aacgaatggt ttgaggtaaa
     3421 gcgctacggt tcttcgattg ataaggtggt caagctttct atgtcttatg gtatctggcg
     3481 tggtgctttc ctggcatttg tcatagccat cgtttttgga gtaatatttt ttattctatg
     3541 gcagggagcc gcactcgtac aaaacggcac catgaacccc ggcgatctgg tttcatttat
     3601 cgtagtgaca ggcattatcg gaggctcgat tggtgggtta ggagatcttt acacacagat
     3661 attgaaagcc ataggggcat ctgaaaggat tcttgaaata atggctatgg attctgaaat
     3721 aaaaatagga cagacatcgg taaagcccat tcctgtcaag ggcaatattc gtttcgagca
     3781 tgtgcggttt gcatacccgg gtcggcctga ggcagaagtg ttgaaagatg tttcgtttga
     3841 aatcaattct ggcgaaaaga ttgctttggt tggttcgagt ggagcaggaa aatctacgat
     3901 tgtgcaactt atcatgcggt tttatgatat caatcaggga aggatactag tagatggcaa
     3961 acgtatcgaa gaatatgata tttcgggcta tagaagcaat ctggccatcg ttcctcagga
     4021 aataatgctc tttggtggta ccatccgcga gaacatactc tatggccgtc cttcagcaaa
     4081 tgaagatgaa gtaagggagg ctgcagaacg atcttttgct atggagttta tcaagaaatt
     4141 tcccgaaggg cttgacacca tcgttggaga gcgtggtatc aaactttcag gtgggcaaag
     4201 acagcgaata gccatagcaa gggcaatact gaaggatccg gctatattga ttctcgatga
     4261 agctacctcc tcacttgatg ccgaatctga aaaacttgta caggcagcgt tag
//
LOCUS       DAOQQY010000244         4262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9739_length_4262_cov_3.995246, whole genome shotgun sequence.
ACCESSION   DAOQQY010000244 DAOQQY010000000
VERSION     DAOQQY010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9739_length_4262_cov_3.995246"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLE23_10430"
     CDS             complement(<1..776)
                     /locus_tag="PLE23_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMX83723.1"
                     /translation="MNQTAYINDVAYTIEPGETMLSFIRKHIGKSHVPTLCDAPNLDP
                     FGACRVCCVDVALTPGGNMKTMASCHTPVIANSYIYTENDRIQKLRKNIVELVLTDHP
                     LDCLTCEVNGNCELQTVAAKVGIRDVRYPEGKNHLHTPKDRSHAYMTSDLSKCINCGR
                     CVRACDEVQGELILSFAGRGFDSHIVKGFDQSFAESDCVSCGACAQACPTSAISDIFE
                     SKSINFTEKTRTVCTYCGVGCNLDVATINGKVKSIQAPYDA"
     gene            complement(825..2474)
                     /locus_tag="PLE23_10435"
     CDS             complement(825..2474)
                     /locus_tag="PLE23_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HMX83724.1"
                     /translation="MSKNLSELSGRKGLDQILFNKLGEISAKSGTPDQADMKKLAEEY
                     LMGNANIYGAASFYDFTREENKGKKIYICNGSACLCAGTQDTLKDKLEEQFKPEEIGE
                     MCCLGRCHENSAFHYNGLNYSGNAIDYLKAGTIVADKNEDSYHTGNIGTPVVNMPFED
                     LDVIKDTIAKALFSTREALIEELKISGLRGRGGAGFPIAFKLESCKNTPGDKKFVVCN
                     ADEGDPGAYSDRYIMEKKPYTLLVGMIAAGYAIGADAGVVYIRREYPESAHIIIKAVQ
                     VLEDAGLLGNNILGSGFNFMFKVVTAQGAYICGEETALLSSIEGQRAEVRTRPPFPTQ
                     LGLFNKPTVVNNVETLANILFILQKGGAAYAAIGKGKSTGTKLISLDSHFNKPGVYEA
                     DMGVPLREVIDKLGGGFKEPIKALHIGGPLGGLVPAQKIDDLTVDFESFSQNGFLLGH
                     ASVICIPQEFPLIKYLEHLFQFSAHESCGKCFPCRLGTQRGYELMHKAIHSDFKVERD
                     LFTDLLDTLERGSLCALGGGLPLPVKNALQYFDDELKQYFN"
     gene            complement(2797..3933)
                     /locus_tag="PLE23_10440"
     CDS             complement(2797..3933)
                     /locus_tag="PLE23_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HMX83725.1"
                     /translation="MQIAVIGAGTMGSGIAQVALMSGCEVILTDTSQASLDRARSGIS
                     DSFVKLREKGKIDIEQSVMYLEKLHCTTQLEAASGADLVIEAIVENLATKKEIFTQLE
                     NIVSPECILASNTSSLSITSIAGTVRHPHRVIGIHFFNPANIMKLVEIIPAIQTDTAV
                     CSKTIDIISSWKKIPVVAKDTPGFIVNRIARPYYSEAIRILEEGIADIATIDLAMTKL
                     AGFRMGPFTLMDFIGHDVNYAVTESVWTACYYEPRYTPSFTQKRLVEAGYLGKKTGRG
                     FYDYRNDAIQVQPNDDFDLLTTISERILVMLFNEAADALYHGIASRDDIEQAMTTGVN
                     YPKGLIAWAQEFGISRCIEKLDSLFDYYKDARYRCSPGLKKLLI"
     gene            complement(3947..>4262)
                     /locus_tag="PLE23_10445"
     CDS             complement(3947..>4262)
                     /locus_tag="PLE23_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMX83726.1"
                     /translation="KASALMMTGEKVPATEAERLGMLYKVFADDIFEDEVKKLAHQMA
                     QMPTKGLGLTKKALNRSLFNDLDSQLQIEDDLQFQAGNTYDYQEGVKAFIEKRKPEFK
                     GK"
BASE COUNT         1030 a          919 c          992 g         1321 t
ORIGIN      
        1 gcatcataag gagcctgtat ggatttgacc ttgccgttga tggtggctac gtcaagattg
       61 cagcctacac cacagtaggt acatactgtt cttgtctttt ctgtgaaatt tatggacttt
      121 gattcgaaaa tatctgaaat ggctgaagta gggcaggcct gggcacaagc tccgcagctc
      181 acacagtccg attctgcaaa tgattggtca aaacccttaa cgatatgcga gtcgaatcca
      241 cgaccggcaa aacttaatat gagttcgcct tgtacctcgt cgcaagcgcg aacgcacctg
      301 ccgcaattga tgcacttcga caagtcggat gtcatgtaag cgtggctacg gtctttggga
      361 gtgtgcaggt gatttttgcc ttccggatag cggacatcac gaatgccgac tttagctgct
      421 actgtttgta gttcgcagtt gccgtttact tcgcaggtga ggcagtcaag tgggtggtcg
      481 gtgagtacca gctctacgat attttttctt agcttttgta ttctgtcgtt ttctgtgtag
      541 atatatgaat tggcgataac cggtgtgtgg catgatgcca tggtcttcat gttgccaccc
      601 ggtgtgaggg ctacgtcaac gcagcagacc cggcatgcac cgaatggatc gagattggga
      661 gcatcgcaca gtgtaggaac gtggcttttg cctatatgct ttctgatgaa gctaagcatg
      721 gtttcgcccg gttctatggt gtaagccaca tcgttgatat atgccgtctg attcataata
      781 ttattggttg aagtgtttat gcaaatgtat tttgttggtt ggggtcagtt gaaatattgt
      841 tttaactcat catcaaaata ttgtagtgca ttcttgacgg gcaatggcag cccgccgcca
      901 agcgcacaaa gcgaaccacg ctcaagtgtg tcgagcaggt cggtgaacaa gtcgcgctca
      961 actttgaaat cagaatgaat ggccttgtgc atgagttcgt agcctcgttg ggtgccgagt
     1021 cggcaaggaa aacatttgcc acagctctca tgtgccgaaa actgaaacaa gtgttcaagg
     1081 tatttgataa gcgggaattc ttgtggaatg caaataacag atgcatgacc aagcagaaaa
     1141 ccgttttgtg aaaatgattc gaaatctact gtcaggtcgt caattttttg tgccggtaca
     1201 agaccaccca aggggccgcc tatatggagt gccttgatgg gttctttgaa tccgccacca
     1261 agcttgtcaa taacttctct aagaggtaca cccatatctg cttcatatac gcccggtttg
     1321 ttgaagtggc tatcgagcga aataagtttg gtacctgttg atttcccttt gccgatggca
     1381 gcgtatgcag cacccccttt ctgcaggatg aaaagaatgt tggcaagggt ttcgacattg
     1441 ttgacgacag ttggtttgtt gaataaccca agctgtgtag ggaatggagg tcgggtacgc
     1501 acttcggcac gttgtccttc gatggaagac agtagggcgg tttcttcacc gcaaatgtag
     1561 gctccctgag cagtaacgac cttaaacata aaattaaagc ccgaaccgag gatgttgttg
     1621 ccaaggagtc cggcatcttc gagcacttgt acggctttta taataatatg agccgattcg
     1681 gggtattctc tgcgaatgta aactacgcct gcatcagcac ctattgcata accggcagct
     1741 atcatgccga ccagtagggt gtaaggcttt ttctccatga tgtacctgtc ggaataagca
     1801 cccgggtcgc cttcatctgc attgcacact acgaattttt tatcacccgg tgtgttttta
     1861 catgattcga gcttgaaggc aatcgggaat cctgcaccac ctcgaccgcg aaggccggat
     1921 attttcagct cttcgatgag ggcttctctg gtagaaaaca atgctttggc aatggtgtct
     1981 tttattacat ccaggtcctc gaaaggcatg ttgactacag gcgtgccgat gtttcctgta
     2041 tggtagctgt cttcattttt gtcggccaca atggttccgg ctttcagata gtcaatggca
     2101 tttcctgaat aattgagacc gttataatga aatgcgctgt tctcatgaca cctgcccaaa
     2161 cagcacattt cgcctatttc ttcaggtttg aactgctcct ctagtttatc tttgagtgta
     2221 tcttgtgtgc cggcgcacag acaagctgaa ccgttgcaga tgtatatttt ttttcctttg
     2281 ttttcttctc tggtaaaatc gtaaaatgac gctgcaccat agatattggc attgcccatc
     2341 aggtattctt cggcaagttt tttcatgtca gcctggtcgg gtgttccact tttggccgat
     2401 atttcaccta acttattgaa aaggatttgg tcaaggcctt ttcttcctga gagctcactt
     2461 aaattttttg acattttcgt tggtttctta aacgctaaca atcatttcaa taataaaaac
     2521 gtccacccga ctcttgttta acccagataa cgcattagat tctgtactgc caataattat
     2581 tacttcaaat ccgtttcaaa agctcaaaat ccatagttca ctatgctttt atcacttttt
     2641 tatgatattt tgtactaatc attttggttt aaacgcctaa tgccttatct gggttaatcg
     2701 gatgatacag atttaagaaa taaaaataac tgctattcgg atgaacacca aatatttaac
     2761 aaaaattaat taaaaaaccg cttctttaga tttattttaa ataagaagtt tttttaggcc
     2821 gggactgcac cggtacctag catctttgta gtaatcgaac aaactgtcaa gcttttcgat
     2881 gcaacggctg atgccaaatt cttgagccca agctatcaat cctttcggat aattaacccc
     2941 tgtggtcatg gcctgttcga tatcgtctct ggatgcaata ccatggtaga gggcatcagc
     3001 tgcttcgttg aacaacatga cgagaatgcg ttcggagatg gtggtcagta ggtcgaaatc
     3061 atcatttggc tggacttgta ttgcatcgtt tcggtaatca taaaatccac gtccggtctt
     3121 tttgcccaaa tagccggctt ctacaagtct tttctgtgtg aaagatggcg tatagcgtgg
     3181 ttcgtaataa caagctgtcc agacgctttc ggtgactgcg tagttgacat cgtgccctat
     3241 gaagtccatc agggtgaatg gccccatcct gaaacctgcc agtttggtca ttgcgaggtc
     3301 tatggtggca atatcggcta tgccttcttc aagtatgcgt atggcctcgc tgtaataagg
     3361 gcgtgcaatg cggttgacga tgaagccggg tgtatctttg gctacgaccg gaattttctt
     3421 ccaggatgaa atgatgtcaa tggttttgga gcatacagct gtgtctgttt gtatggccgg
     3481 gatgatctcc acaagcttca tgatgttggc aggattgaag aaatgtatgc caatgaccct
     3541 gtgcggatgt ctgactgtac cggcaatgga tgttatcgaa agggatgatg tgttgctggc
     3601 aagaatacac tcaggcgaaa cgatgttttc cagttgtgtg aatatttctt tcttggtagc
     3661 gaggttttca acgatggcct ctattaccag atctgcaccg gaggctgctt ccaattgggt
     3721 agtacaatgc agtttttcaa ggtacatgac cgactgttcg atgtcgattt tacctttttc
     3781 gcgaagctta acaaaactgt cgcttatacc ggaacgtgcc ctgtcaagtg aagcttgact
     3841 tgtgtcggtt aggatgactt cacaacccga catgagtgct acttgtgcta ttcctgagcc
     3901 cattgtaccg gctcctatga ctgctatctg catagatttg cctattttat tttcctttaa
     3961 attctggttt tctcttttcg ataaatgctt ttacaccttc ctgataatcg tatgtgttac
     4021 cagcctgaaa ctggaggtcg tcttctatct gcagttgtga gtcaaggtca ttaaacaatg
     4081 atctgttaag cgcttttttt gtgaggccta gcccttttgt aggcatttgt gccatttggt
     4141 gtgccaattt ttttacttca tcttcaaata tgtcatctgc gaataccttg tacagcatgc
     4201 ctagccgttc tgcttcagtt gccggcactt tttctcctgt catcataagg gcagatgctt
     4261 tc
//
LOCUS       DAOQQY010000245         4245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9797_length_4245_cov_6.291885, whole genome shotgun sequence.
ACCESSION   DAOQQY010000245 DAOQQY010000000
VERSION     DAOQQY010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9797_length_4245_cov_6.291885"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PLE23_10450"
     CDS             <1..202
                     /locus_tag="PLE23_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HMX83727.1"
                     /translation="NALLDYGRPEKVELLVLVDRRFNRELPVSSDYTGTTIDALSQEY
                     VKVEWKQLHGEDRILFLSGKQQ"
     gene            206..1138
                     /locus_tag="PLE23_10455"
     CDS             206..1138
                     /locus_tag="PLE23_10455"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HMX83728.1"
                     /translation="MSANQISTRHLLGIKDINEQDIELIFNTAREFKEVINRPIKKVP
                     SLRDITIANIFFENSTRTKLSFELAEKRLSADVVNFAASSSSVSKGETLLDTVNNILS
                     MKVDMVVMRHPAPGAPAFLAKHIQANIVNAGDGTHEHPTQALLDAYSIYEALGDIAGK
                     KVAIIGDILHSRVALSNIYCLKKLGAHVKVCGPPTLIPKHIASLGVEISYNIDDTLQW
                     CDVANVLRIQLERQEIKYLPSLREYALYYGINKARLDKLSKKTVIMHPGPINRGVEIT
                     SDVADSEHSIILQQVENGVAIRMAVLYLLASRKA"
     gene            1234..2667
                     /locus_tag="PLE23_10460"
     CDS             1234..2667
                     /locus_tag="PLE23_10460"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMX83729.1"
                     /translation="MKLLYKIFIFSAFSIFASYTVNADNGYEIKVKLKNYTQDKLVLG
                     FQLGDKSYIKDSAIVDQNGWFTFKNEKNLDPGVYIVLMKPDNQYFQILVDTDDQHFSF
                     EADGKEPLTTFKAKNSKYNNIFYDYLRYIDGKRHEVEPLQKVVSGDNPESAKKKAQEG
                     LDKINDDVRKYQDNLAAKNPNTVMALLVKASQDIEVPQFEGVSDSINKVKRYFYYKDH
                     YFDNIDLSNIKLLKTNVLFQKVDYYIQKLVVQHPDTVNLAVKRVLDKMAPTEEVFKFY
                     LIHYLNFYAKSNIVGFDAVYVFIVENYYAKGMAPWTEKEQLDKITKEARSIKPTLIGK
                     TAPNIKMYKQDNTPVSLYDVKSDFVVLLFWNPDCGHCKKEMPDVVAMEKRFKDKGVTV
                     FSVCTALKDKVGECWNFIKDKEMQDFLNVVDPEVTSGYYTLYKVTQTPLTFILDKDKK
                     IVSKKISPEQLDEVLEHFIEEKHKTDK"
     gene            complement(2692..3699)
                     /gene="tsaD"
                     /locus_tag="PLE23_10465"
     CDS             complement(2692..3699)
                     /gene="tsaD"
                     /locus_tag="PLE23_10465"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMX83730.1"
                     /translation="MKLKENPVILAIESSCDDTSAAVFESGKMLSNLIATQAIHEQYG
                     GVVPEVASRAHQEHILPVVDTALKKAGKKLNDIDCVAVTAGPGLMGSLLVGVTFAKSL
                     ALCLDVPLIDVHHMEAHVLAHFIDDPQPKFPFLNLTVSGGHTQIVLVKDFFDMKVVGT
                     TIDDAAGEAFDKSGKILGLGYPAGPLIDKHAALGVAKFSFPEPQIANLDFSFSGLKTS
                     ILYFLKEQQLANKDFINENLDDICASVQSRIISILIKKLTRAVRQYKVDEIGISGGVS
                     ANSGLRKAIMETGEKLNLRVHIPKFEYCTDNAAMIAMAAYYKYKAGLFADQALVPFTR
                     S"
     gene            3801..>4245
                     /locus_tag="PLE23_10470"
     CDS             3801..>4245
                     /locus_tag="PLE23_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83731.1"
                     /translation="MPAFLLFLTISSAKIWYVVPGTSIPDPKTAAGLASSGDTIDIAY
                     SAVPYLAYETKWPQHNLLIRGSGTSRPILKAETFSVTQKAIFVIQGNDVTIENIEFTN
                     ARVPDKNGAGIRSEGVNLNVRHCTFRTNETGILAGDKPDCKILVEY"
BASE COUNT         1356 a          819 c          852 g         1218 t
ORIGIN      
        1 caatgccttg ctggattacg gaagacccga aaaagtagaa ttgcttgtat tggttgaccg
       61 gaggtttaac cgcgaattgc ctgtgtcaag tgattataca ggtactacca ttgatgcatt
      121 gtcgcaggaa tatgtgaaag tagaatggaa acaacttcac ggtgaagacc gaatattatt
      181 tttatcaggc aaacaacaat aattaatgtc agcgaaccag atcagtacca gacatttatt
      241 aggaataaag gatataaacg aacaggatat tgaactcata ttcaatactg cacgtgagtt
      301 caaagaggtg atcaatcgcc cgattaagaa ggtaccctcg ctcagagata tcacaattgc
      361 caatatattt tttgagaatt cgacacgtac aaaactttca tttgaactgg cagaaaaacg
      421 tctgtctgct gatgtagtta attttgctgc gagctcctct tctgtaagta aaggtgaaac
      481 tttgcttgat acagtcaata atatcctttc aatgaaagtg gatatggtgg tgatgcgtca
      541 tccggctccg ggtgctcctg catttttagc caaacatatt caggcgaata ttgtcaatgc
      601 aggcgatggc acacacgaac accctacgca ggcattgcta gacgcatatt ctatctatga
      661 agcattgggc gacattgcag gtaaaaaagt tgctatcatt ggcgacatcc ttcattcacg
      721 tgtggcgctg agcaatattt attgtcttaa aaaactgggt gcacatgtaa aggtgtgtgg
      781 cccgcctaca ctaataccca aacacattgc gtcacttggt gtagagatta gctacaatat
      841 agatgatacg ctgcaatggt gcgatgtagc caatgtgctt cgaatacagc tcgaacgaca
      901 ggaaatcaaa tatttgcctt cattacgaga gtatgcccta tattatggaa ttaataaagc
      961 aaggctggac aaactatcaa aaaaaaccgt tatcatgcac cctggaccta ttaaccgtgg
     1021 tgttgagata acgagcgatg tagctgattc tgaacatagt atcattctgc aacaggtaga
     1081 aaatggtgtg gctatcagga tggctgtact ctatttgctc gcatcacgca aagcctgatt
     1141 tcttgatatt aaccttactt taacataaca aaaagtggcc aaattgcgtc taatgatagt
     1201 atttttaccg tcagacttaa atattttatt acaatgaaat tgctttacaa aatattcatc
     1261 ttttctgctt tctcaatttt tgcttcatac actgtcaatg cagacaatgg atatgaaatc
     1321 aaggtcaaat taaagaatta tacccaagat aaacttgttc ttggttttca gcttggagat
     1381 aaatcttata tcaaagactc tgcgatagtt gaccaaaatg gttggtttac atttaaaaat
     1441 gaaaaaaatc tcgatccggg tgtatatatc gtactcatga agcctgataa tcagtatttt
     1501 caaatactcg ttgacacgga tgaccagcat ttttcattcg aagcagatgg caaagaacca
     1561 ctcacaacat ttaaggcaaa aaatagcaaa tacaacaaca ttttctacga ttaccttcgg
     1621 tacattgatg gaaaaagaca tgaagtagaa ccacttcaga aagttgtatc cggtgataat
     1681 ccggaatcgg ccaaaaaaaa ggctcaggaa ggtctggata aaattaatga tgatgtaaga
     1741 aagtatcaag acaatctggc agctaaaaat ccgaatacgg taatggcact attggttaaa
     1801 gccagccagg atatagaagt gccgcagttt gaaggtgttt ctgatagtat caataaggta
     1861 aaacgttatt tttactacaa agatcattac ttcgacaata ttgatttgtc aaatattaag
     1921 cttttgaaaa ccaatgtgtt gtttcagaaa gtggattact atattcaaaa actcgtagtg
     1981 cagcaccccg ataccgtaaa tcttgcagta aagcgggtat tagacaagat ggcgccgaca
     2041 gaagaggtat tcaaatttta ccttatacac tacctaaact tctacgcaaa atcaaatatt
     2101 gtcggctttg atgctgtata tgtctttatc gttgaaaatt attatgccaa aggtatggcg
     2161 ccttggacag agaaagaaca actcgataaa ataaccaagg aagcacgttc gataaagcct
     2221 actttgattg gcaagactgc tcctaatatt aaaatgtata agcaagacaa tacaccggtc
     2281 tctttgtatg acgtaaaatc agattttgta gtattgttat tctggaatcc cgattgtggc
     2341 cactgcaaaa aagagatgcc cgatgtagtt gcaatggaga agagattcaa agataaaggc
     2401 gtaacggttt tctctgtttg tactgcactt aaggacaaag tgggtgaatg ttggaatttc
     2461 ataaaagata aagaaatgca ggattttctg aatgtcgtgg atccggaggt gacgagtgga
     2521 tactatactt tgtataaggt gactcaaaca ccactgacat ttattcttga caaagacaag
     2581 aaaattgtat ccaagaaaat ttcaccggaa caactcgacg aagtactcga acatttcatc
     2641 gaagaaaaac ataagactga taaataagtc tctgtatttt tctgtatagc tttaagatct
     2701 tgtaaatggc acaagtgctt gatcggcaaa taagccggcc ttatatttat aatatgcagc
     2761 catagcaatc atagcggcat tgtctgtaca atattcaaat ttcggtatat ggactcttag
     2821 attcagtttt tctccggttt ccatgatagc ttttctcaat ccggaatttg cagacacacc
     2881 acctgaaatg ccgatttcgt caaccttgta ctgcctgaca gctcttgtga gtttttttat
     2941 taaaatagaa ataatccgtg actgaactga agcacaaatg tcatccagat tttcgttgat
     3001 gaaatccttg tttgctagct gctgttcttt cagaaaatat aaaatcgagg ttttaaggcc
     3061 tgaaaaacta aagtccaaat ttgcaatttg tggctcggga aatgaaaatt ttgcaacacc
     3121 aagtgccgca tgtttgtcaa taagcggacc ggcaggataa cctagaccca atatttttcc
     3181 cgatttgtca aaggcttctc cggctgcatc atcgatggtt gtgcctacga ccttcatatc
     3241 aaagaaatct tttaccagca ctatttgtgt gtgtccgccc gaaacggtga gattaagaaa
     3301 aggaaacttt ggctgtgggt cgtctatgaa atgtgccaaa acatgggctt ccatgtggtg
     3361 tacatcaatg agtggcacat caagacataa agcaagtgat ttggcaaagg taacgcctac
     3421 cagtagcgaa cccatgagcc ccggaccggc agtaacagca acacagtcaa tatcattgag
     3481 cttttttcct gccttcttta aagcagtatc cactaccggc aatatgtgtt cctgatgtgc
     3541 acgtgaggcc acttcgggta caacgccgcc gtattgttcg tgtatagctt gtgtggctat
     3601 cagattgctc aacatctttc ctgattcgaa tacagcagct gaggtatcat cacaagaaga
     3661 ctcaatggcc agtatgaccg gattttcttt taatttcatt acacaaattt atggcaatat
     3721 tgggttagtt ttactgaatg ttgctatatt caatttttat ttacattaaa atactacaat
     3781 gttaaaaaaa actcctgatt ttgcctgcat ttttactgtt tctgaccatt tcttctgcaa
     3841 aaatctggta tgtagtaccc ggtacttcca ttcctgatcc aaaaactgca gctggtttgg
     3901 cttcgagtgg agacaccatt gacattgcct atagtgcagt accttacctg gcttatgaaa
     3961 ccaaatggcc acaacacaat ctattgataa gaggaagcgg aacatcaagg cccatactca
     4021 aagcagagac tttttcggta actcagaaag ccatttttgt aatccagggc aatgatgtta
     4081 cgatcgaaaa tatcgaattc accaatgcac gtgtaccgga caagaacggt gccggcatca
     4141 ggtcggaagg tgttaacctc aatgtgcgcc actgtacatt ccgcaccaac gaaaccggca
     4201 tactggcagg tgacaaaccg gattgcaaaa tactggttga atact
//
LOCUS       DAOQQY010000246         4231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_9843_length_4231_cov_5.210728, whole genome shotgun sequence.
ACCESSION   DAOQQY010000246 DAOQQY010000000
VERSION     DAOQQY010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9843_length_4231_cov_5.210728"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..655)
                     /locus_tag="PLE23_10475"
     CDS             complement(83..655)
                     /locus_tag="PLE23_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83732.1"
                     /translation="MEVDGHFPYHPYSYHTSKVQIGQIDEVIIDSLPRKRMEIKGVDW
                     MYGAPEYWIEGIGSTLGFLSSGITGMGIFDICYPTLLCLEQDSVITYQDPVVKKCYQK
                     CKPGATGEVPIPEKGKIYPHPASSAISLESADIIEEYSISTISGLPIVHERCGAHKVT
                     ANVAGIQNGIYFITIMTKVGISVQKLVIAR"
     gene            complement(799..966)
                     /locus_tag="PLE23_10480"
     CDS             complement(799..966)
                     /locus_tag="PLE23_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83733.1"
                     /translation="MKKYLLFMAAILFAMSLSAQYIPLPDSNAVWSVDFVKYSIEGDS
                     TYKSKTIRKFI"
     gene            1269..2564
                     /locus_tag="PLE23_10485"
     CDS             1269..2564
                     /locus_tag="PLE23_10485"
                     /inference="COORDINATES: protein motif:HMM:NF021427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HMX83734.1"
                     /translation="MLNEIICPNCKKAFKVDEAGFAEILKQVRDHQFEEELAKLHNRA
                     EQDKQNAVKLAEANLRQSLQNQLNAKDNELNLLKASHEAQLAQKLAQKESELTQLKAK
                     IGQAELEKKLSVTQAIQHLEKERDSLQNTIKNKDLEKELLSKALQEKFAAELNAKDNI
                     IRLKDEEIELRKDMKLKLSTKMLGETLEQHCETEFNKLRATAFPNAYFEKDNDSSSGS
                     KGDFIFRETNHAGIEIVSIMFEMKNEADLTATKKRNEDFLKELDKDRNEKKCEYAVLV
                     SLLELDNELYNSGIVDVSHKYPKMYVVRPQFFIPIITLLRNAALNSVQYKHELALVRS
                     QNIDITNFEEKMNKFKEGFARNYDLASRKFKDAIDGIDKTIKELEKTKAALLSSENNL
                     RLANEKTEDLTIKKLTHGNPTMKQKFEELKGGANILGEE"
     gene            2711..4087
                     /locus_tag="PLE23_10490"
     CDS             2711..4087
                     /locus_tag="PLE23_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83735.1"
                     /translation="MSKRIFPHLAVIIFFVIAIFVDFNHHPWTSKDVLRYDRFGYHNY
                     LPALFIYNDITKYNFIDNIEKLYPSTNGEQKRYGLHQVEKTGNLCNQYPIGVAVFQAP
                     LFLVAHLWATVSGRDKSDGYSAAYQHSVVLSTLLFATLGLLILSRFLRKYFSDVVVFF
                     TILLIAFGTNFFHYATLESGMAHIYQFFLYSSILYLTYQWYRQPAFKTSALIGICTGL
                     AIITRPTDLLICIIPLSWASTMPGKWDFIRQNMKYVWLIVLFTFLFCLPQMLYWKYVT
                     GDWVYYSYNGGDYFRFDRLRIIQGLFSYRKGWFVYTPLALLAFIYMWGVRKDSAFYSY
                     KKTFWIFFIPMIYLVFSWNNWFYGWSYGCRALLGTLPLLAIPLALMVQDIATSKLLRK
                     LIAVTGVIFITFLNLFQTWQYEHYILHGALMNESTYWALFLKTEKPPDFDKNFKLQEE
                     LDWNTGGF"
BASE COUNT         1323 a          847 c          801 g         1260 t
ORIGIN      
        1 ttgagctttt aaaatggatt tgaagtaata attatttaca gtaaagagtc caatacgttt
       61 tctgggttta tgcagtatgt gtttacctcg ctatcacaag cttttgtacg cttataccaa
      121 cttttgtcat gatggtgatg aaataaatgc cgttttgtat gccggcaaca tttgccgtta
      181 ccttgtgtgc accacatctt tcgtgcacaa tgggtagtcc gctgattgtg ctaatactgt
      241 attcttcaat aatatcagcc gattctaagc ttatggcgga cgaagccgga tgtgggtata
      301 tcttgccctt ttcaggtata ggtacttcgc cggtagcacc tggtttacac ttctggtagc
      361 atttttttac tacaggatct tggtaggtga tgacactgtc ctgctccaaa catagaagtg
      421 tagggtaaca gatatcaaaa atgcccatac cagttatacc cgaacttagg aatccaagag
      481 tgctgccaat accttcaatc caatattctg gtgcaccgta catccaatct acacctttta
      541 tttccatcct tttacgtggg agggagtcta ttatcacttc atcaatctgg ccaatctgta
      601 ccttacttgt gtggtatgaa taagggtgat acggaaaatg tccgtcaact tccacaatat
      661 catcggtttc taagctgaag tcgtataaca gtttttctta aggatcattt ttgaatatta
      721 caaatacttt ttgtgatggt ttgtcctgcc tgatgagtgc aaaaaactct tttttgatag
      781 ttggataact gtaattggct atataaattt tcttatcgtc ttggatttgt aagtcgaatc
      841 accttcaata gaatacttta caaaatcgac cgaccatacg gcatttgagt ctggcaatgg
      901 aatatattgg gctgataatg acattgcaaa caatattgcg gccataaaaa gtaaatattt
      961 tttcatattg aggcgatttt gatggtgaaa tatgaataat acaaaatata ctgatgaagt
     1021 acattttgcc aatcctacaa cacaaatata aggaataatt ttatcaaaat aaaaaaaatc
     1081 aaataatatt attctaaaat cattgtaaaa taatcgtata aattttaaat tgattgttca
     1141 ataatgttat ttattctgat acaagttttt tgtttccaac cggcactttc attatctcga
     1201 tttttttgtg taaattgcaa tccgttattt gacaccatct atttatacat tatatataca
     1261 ctataatcat gctgaacgaa attatttgcc ccaattgtaa aaaagccttc aaagtggatg
     1321 aggctggctt cgcagaaata ctcaaacagg tacgcgacca tcagtttgaa gaagaattgg
     1381 caaaactcca taaccgtgcc gaacaggaca aacaaaatgc cgtaaaactc gccgaagcaa
     1441 acctgcgaca gtcactccag aaccagctca atgcaaagga caacgaactt aacttgctca
     1501 aagcttcgca cgaagcccaa ctcgcccaaa aactggctca aaaggaatcg gaacttactc
     1561 agctgaaggc taaaattgga caagccgagc tagaaaagaa gctttccgtt acacaagcca
     1621 ttcagcatct cgagaaagaa cgcgatagcc tgcaaaatac catcaaaaac aaggatctcg
     1681 aaaaagaact cctttcaaaa gcactacaag aaaaatttgc tgccgaactc aatgccaagg
     1741 ataacatcat caggcttaaa gatgaagaga tagagctgcg caaagatatg aaactcaaac
     1801 tctctactaa aatgctcggc gaaacactcg aacaacattg cgaaactgag tttaacaaac
     1861 tgcgtgccac tgcattcccg aatgcttatt tcgaaaaaga caacgactcg agctcaggta
     1921 gcaaaggcga tttcatcttc cgcgaaacaa atcatgccgg tattgaaatt gtatccatca
     1981 tgttcgagat gaaaaacgaa gccgatctca cagctacgaa gaaaagaaat gaggactttc
     2041 tgaaagaact agacaaagat cgcaatgaga aaaaatgtga gtacgctgta ctggtttcat
     2101 tacttgaact cgataacgag ctctataatt ccggtatagt ggatgtctct cacaaatatc
     2161 cgaaaatgta tgtcgtaaga ccacagtttt ttattcccat cataacattg ctccgaaatg
     2221 ccgctctcaa ctctgtacag tataagcacg agcttgccct cgttcggagt cagaatatcg
     2281 atatcactaa ttttgaggaa aaaatgaaca agttcaagga aggttttgca agaaattacg
     2341 acctggcaag cagaaagttc aaagatgcca tcgacggaat cgacaaaaca atcaaagaac
     2401 tcgaaaaaac caaagcagcc ttactctctt ctgagaacaa cctcagactc gccaatgaaa
     2461 aaaccgaaga cctcaccatt aaaaaactta cacatggcaa tccgactatg aagcaaaaat
     2521 tcgaagaact gaaaggaggc gcaaatatat taggtgaaga atgaaaatag atgaggaaat
     2581 acaggctttt gattcttttt aagtgaaatg aatcaacatt ctttgttatg ttacgaggtt
     2641 caaaagataa tcttatcttt gggccattca tttacgttat agattcaaca aacaattaat
     2701 agacttagca ttgagcaaaa ggattttccc acaccttgcg gtaatcattt tttttgtaat
     2761 agccatcttc gtggatttta atcaccatcc atggacatca aaagatgttc tacgatacga
     2821 taggtttggt tatcacaact atttaccagc tttgttcatt tacaacgata taacaaagta
     2881 caacttcatt gacaacatcg aaaaactata cccttctact aatggtgagc aaaagcgtta
     2941 tggtcttcat caggtagaaa agacgggcaa cttatgcaat cagtacccaa taggagtggc
     3001 agtttttcag gcaccattat ttttggttgc acatctgtgg gcaaccgttt caggacggga
     3061 caaatctgat ggatactccg ctgcctacca gcattcagtc gtgctttcta cactactctt
     3121 cgcaacgctt ggcttattga tactcagccg gtttttgagg aaatattttt cggacgtagt
     3181 agtgtttttt acaattctgc tgattgcttt tggtaccaat tttttccatt atgctacact
     3241 tgagagtggt atggcacaca tttaccagtt ttttctgtat tcgagcatac tgtaccttac
     3301 atatcaatgg taccggcagc ctgccttcaa gacttctgct cttattggca tttgtacagg
     3361 tctggctatc attacccggc ctaccgatct gctcatttgt attattccac ttagctgggc
     3421 atcaaccatg ccgggcaaat gggatttcat aaggcagaat atgaagtatg tctggttaat
     3481 cgtattattc accttcctgt tttgtctgcc acagatgtta tactggaaat acgtgaccgg
     3541 cgactgggtc tattattctt ataacggagg cgattatttc cgcttcgaca gactacggat
     3601 tatacagggt ctgtttagct accgcaaagg ttggtttgta tatactccgc ttgccttgct
     3661 tgcatttatc tatatgtggg gcgtgcgcaa agattctgcg ttttattcct acaaaaaaac
     3721 attctggatt ttcttcatac caatgattta ccttgttttc tcgtggaata actggtttta
     3781 cggctggagt tatggctgtc gtgcattatt aggcacgttg cctttgctgg ccataccctt
     3841 ggctctgatg gtgcaggata ttgctacaag caagttgtta aggaaattga tagccgttac
     3901 aggtgttatt ttcattacct ttctaaatct tttccagacc tggcaatatg agcattatat
     3961 actgcatggt gcgcttatga atgaatctac atactgggcc ttgttcctca agacagaaaa
     4021 gccgccggat tttgacaaaa actttaagct gcaggaagaa ctcgactgga atacgggcgg
     4081 attctaatat ccttgaagca aagtacagaa agtggcttag acagaatccg ggttaaattt
     4141 gaaacaagag tttcacagct tcatctatac cttctacagc cgttttttgg atgaaataga
     4201 caaggaatat attgaaatgg acagaaggtt t
//
LOCUS       DAOQQY010000247         4189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10010_length_4189_cov_6.136672, whole genome shotgun sequence.
ACCESSION   DAOQQY010000247 DAOQQY010000000
VERSION     DAOQQY010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10010_length_4189_cov_6.136672"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PLE23_10495"
     CDS             <1..318
                     /locus_tag="PLE23_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539494.1"
                     /note="catalyzes the thiolytic cleavage of
                     beta-ketoadipyl-CoA to succinate and acetyl-CoA; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HMX83736.1"
                     /translation="IGPVEASRLALSRAGLSLAQMDIIEINEAFAAQVLACTRSLGLE
                     DKDERINPNGGAIAIGHPLGMSGTRILHSASIELSRRNARYALVTMCIGVGQGYAVIL
                     EKA"
     gene            complement(354..533)
                     /locus_tag="PLE23_10500"
     CDS             complement(354..533)
                     /locus_tag="PLE23_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83737.1"
                     /translation="MQNGKEEGIQEGIQKGIQKGKIEVILTGFDNGANIAFLSTITHL
                     SEAEVTEILEKHGKI"
     gene            complement(692..3208)
                     /locus_tag="PLE23_10505"
     CDS             complement(692..3208)
                     /locus_tag="PLE23_10505"
                     /inference="COORDINATES: protein motif:HMM:NF036809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83738.1"
                     /translation="MKLKFITILLSFIGLISFHHFADAQETFKEIRLDDWMVSKTNRD
                     MWFPAKVPASVHTAMYEAKRIPDPFKGDIEERLQFIGQSDWTYQTTLHITEQQLKAAQ
                     ILLEFKGLDTYSDIYINNKKTFTTDNMFRTWQFDIKSFIHTGDNEIKLIFHAPLVFSE
                     KYQESLNIDFPGGPRVWARKAQYQFGWDWAPTYLTMGIWRPVYVKFAYQPYITATDIN
                     YRIEGTTADIEISADIMSFSKIHYHTEAQIAGQSQKKAIELKEGLNKVNFSFKIENVE
                     LWWPNGMGEQKMYHYQVAIGKKKVGEQVVNGKTGFRKVELITIADKAGESFYFKINNR
                     PVFAKGANYVPADAFPEQITDTRLTALLSDAKKSNFNMLRVWGGGIYESNRFYELCDS
                     LGLMIWQDFMFACAMYPGEEFFLENIRKEAEENVERIARHPCVVLWCGNNENAEGWAR
                     WGWQDKLSTEQKKYLETAYSKVFDEILPQTVRQYGKKIPYWESSPKFGRGDIRHRFEG
                     DAHYWGVWHDGEDFSKYEEKVPRFMSEFGFQSYPEMSTIRYFSGMELKQDEPSMLAHQ
                     KHPRGNQLIDQYIRRTFNPADDFESFVYLSQVIQAEGVGRGIAAQRTAMPFCMGSLYW
                     QLNDCWPAISWSSIDYFGKWKPLQYKAKELFKPVIVNTRVQNDKITTNIVSDIDSTLS
                     LDLEILVYTTDGTLVNKIPQDINITSAGSVQVFENNISEETKKFGAANLCYVSRLYNR
                     SKTIISEDCDFAVLPKDLNLSKDKLTFTVKEIPEGYLIEITSTVVNPGVFLTTDLRGT
                     FSDNYFNMLPGTKTVTFKTDEPSKNPEKAFQVMTWTKATK"
     gene            complement(3205..>4189)
                     /gene="lhgO"
                     /locus_tag="PLE23_10510"
     CDS             complement(3205..>4189)
                     /gene="lhgO"
                     /locus_tag="PLE23_10510"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HMX83739.1"
                     /translation="LLQFAAEHSIPHDICGKIIVATNKSELQALDNIYHRGLANDLKG
                     IYKMNPDEVKEREPHVKAEAAIWVPQTGIIDYKEFSARLLQLIQHQGGEIFLNNKVEK
                     IENDQSSFKITTSRAVYITSFVINCAGLYSDHIAQMTGQAQDLQIIPFRGEYFMLKPE
                     KEYLVNNLIYPVPDPNFPFLGVHFTRLIHGGREAGPNAVLAFRREGYNKSDIHFRELF
                     DILRFPGFHKIMKKYWREGLGEMYRSWSKTAFTKALQKLIPDIQKEDLVRGGSGVRAQ
                     TVDIEGNLSDDFVIFDQPGVLNVCNAPSPAATSSLSIGSTIANSYFKNKKR"
BASE COUNT         1107 a          870 c          835 g         1377 t
ORIGIN      
        1 atcgggcctg tagaagcatc caggctggca ctcagcagag cgggtttgag cttggctcag
       61 atggatatta tcgaaattaa tgaagcgttt gcagcacagg ttctggcttg taccaggagc
      121 ctcggcttgg aggataaaga tgaacgaata aatcctaacg gaggtgcaat agctattggt
      181 catccacttg gaatgtctgg tacccggata ctccactcag cttctattga actatccagg
      241 cgcaatgcca gatatgcact tgtaactatg tgtataggtg tggggcaggg ttatgcggta
      301 atattagaaa aggcctgaag aaattcatta agaagaaaaa tattaaggca cacttatatc
      361 ttaccgtgtt tctccaatat ttctgtaacc tcagcttcgc ttaaatgcgt tattgtgctc
      421 aaaaatgcaa tattggcacc attgtcaaag ccggtcagga ttacttcaat cttccctttc
      481 tgaatacctt tctgaatgcc ttcttgaatc ccttcttctt tgccattttg caaatctacc
      541 taaaatcagg tacaagattt gaaataaatg tagattaaat cttatacaaa ttggcttttc
      601 ttgatatcac ttgcgtatgc aacttggttt gattactcca caaaataaca tgggtgtata
      661 tgttcatata ttgctaatca tgattagatg attatttggt tgcttttgtc caagtcatta
      721 cttgaaaggc tttttcagga tttttagaag gctcgtcggt cttgaatgta actgttttgg
      781 ttcctggtag catgttgaaa taattatcac taaatgttcc tctgagatcg gtggtaagaa
      841 atacacccgg attaactaca gttgatgtta tttctattaa atacccttcg ggtatttcct
      901 taactgtaaa tgtcagctta tcttttgaca gattcaaatc tttgggcaac actgcgaaat
      961 cacaatcttc ggagataatt gtttttgatc ggttataaag tctgcttaca tagcacagat
     1021 tggcggcacc aaattttttt gtttcttctg aaatattatt ctcgaaaacc tgaactgaac
     1081 cggcggaagt aatgtttatg tcctgcggga ttttattaac cagtgtgcca tcagttgtat
     1141 aaaccaatat ctcaaggtca agactaagtg tactatcgat gtcagaaacg atgttggtcg
     1201 taattttatc attttgcacc cgagtattga ctatgaccgg cttgaacagt tctttggctt
     1261 tgtattgcaa tggtttccat ttaccaaaat aatcaatact gctccaggag attgctggcc
     1321 aacaatcgtt caattgccag taaaggctgc ccatacaaaa aggcattgca gtacgctgag
     1381 cggctatacc acgacctact ccttcggctt gtattacttg cgacagatag acaaatgatt
     1441 caaaatcatc tgccggattg aatgttcttc tgatgtattg gtcaataagc tggttgccac
     1501 gtggatgttt ctggtgggcc agcatacttg gctcatcttg ttttagctcc ataccggaaa
     1561 aatatcgaat ggtgctcatt tcaggatatg actggaatcc gaactccgac ataaatcttg
     1621 gtaccttttc ttcatattta ctgaaatctt caccgtcatg ccaaacaccc caatagtgtg
     1681 catcaccttc aaaccggtgt cggatgtcac ctcgcccgaa ttttggtgat gattcccagt
     1741 agggtatttt ttttccgtat tgccttacag tctgaggcag aatttcgtca aataccttgc
     1801 tgtaggctgt ttcaaggtat tttttttgct cggtagagag cttgtcttgc caaccccaac
     1861 gtgcccatcc ttcggcattt tcattgttac cgcaccaaag tacaacacaa gggtgtcggg
     1921 ctattctttc tacattttct tcagcctctt tcctaatatt ctccagaaaa aactcctctc
     1981 ccgggtacat agcacacgca aacataaagt cctgccagat catgagtcca agtgaatcac
     2041 acaattcata gaaccgatta ctttcataga tgccacctcc ccaaacacgt agcatgttga
     2101 aatttgattt ttttgcatca gacaacaaag ctgtcaatcg tgtgtctgta atttgttcag
     2161 ggaaagcatc tgcaggtaca tagttggctc cttttgcaaa gacaggtcta ttattaattt
     2221 taaaatagaa actttcacct gctttgtcag caatggttat tagttctact ttcctgaacc
     2281 ctgtttttcc attcacaact tgttcgccta cttttttctt gccaatggcc acttggtaat
     2341 ggtacatctt ctgctcgccc atgccgtttg gccaccagag ttcaacattt tctatcttaa
     2401 aactgaaatt gactttgttc aagccttctt taagttcaat cgcttttttc tggctttgtc
     2461 ccgcaatctg agcttcggta tggtaatgta tttttgagaa actcataata tcagcagaaa
     2521 tttcaatatc agcagtcgta ccttcgatgc ggtaattgat gtctgttgca gttatataag
     2581 gctgatatgc aaacttaacg tacaccggcc gccagatacc catggtcaaa tatgtcggtg
     2641 cccagtccca gccaaactga tattgcgctt tgcgcgccca gacacgcgga ccacccggaa
     2701 aatcgatgtt taatgattcc tgatatttct ctgaaaaaac cagaggggca tgaaatatta
     2761 gcttgatctc attatcgccg gtgtgtatga atgattttat gtcgaactgc catgtcctga
     2821 acatattatc cgttgtaaat gtttttttgt tattgatgta aatgtctgaa taagtatcca
     2881 atcctttaaa ttcgagaagt atttgggctg cttttagctg ttgttcagtt atgtgaagcg
     2941 tagtctggta tgtccaatcg ctttgcccta taaattgcaa tctctcttct atatctcctt
     3001 tgaatggatc gggaattcgt tttgcttcat acatagctgt atgtacagaa gcaggtacct
     3061 ttgcaggaaa ccacatgtcg cggttggttt tagaaaccat ccaatcatcc agtctaatct
     3121 ctttaaacgt ttcttgggca tctgcgaaat gatggaaact aatgagtcca ataaagctta
     3181 gcagaatggt aatgaatttt aacttcatct ttttttgttt ttaaaataac tgttggcaat
     3241 ggtactgcct attgaaagtg aagacgtagc tgccggcgat ggcgcattac agacattcag
     3301 cacaccgggt tggtcaaata ttacaaaatc gtctgaaaga ttcccttcga tatcaactgt
     3361 ttgtgctctt actcctgaac caccccggac aaggtcttct ttctgtatgt cgggaatgag
     3421 tttttgcagt gcttttgtaa atgcggtttt tgaccacgat ctgtacattt cacccaaacc
     3481 ctctctccag tattttttca tgattttatg aaaaccggga aagcgcagga tgtcaaaaag
     3541 ctccctgaaa tgaatatctg atttgttgta accttctctt ctgaatgcaa gaacggcatt
     3601 gggtccggcc tctcgtccgc catggatcaa ccttgtaaaa tgtacgccaa ggaaaggaaa
     3661 attcggatcg ggtacgggat aaatcaggtt gttaaccaga tattcttttt cgggtttaag
     3721 cataaaatat tctcccctga atggtataat ctgaaggtcc tgagcctgtc cggtcatctg
     3781 agctatgtgg tcggagtata gtccggcaca gttgatgacg aacgatgtta tgtatacggc
     3841 acgggacgtt gtaatcttaa aacttgattg atcgttttcg attttttcta ccttattatt
     3901 cagaaaaatt tcaccacctt ggtgctggat aagttgcaaa agtctggcag aaaattcttt
     3961 atagtcaata atgcctgtct gtggtaccca aatagccgct tcagccttta cgtgtggttc
     4021 gcgttccttt acttcgtcgg gattcatttt gtatatgcct ttgaggtcgt tggccagacc
     4081 tctgtggtat atattatcta atgcctggag ttctgattta ttggtagcaa ctattatttt
     4141 tccgcaaata tcatgtggta tgctatgttc tgctgcaaac tgcagcagt
//
LOCUS       DAOQQY010000248         4188 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10015_length_4188_cov_5.509799, whole genome shotgun sequence.
ACCESSION   DAOQQY010000248 DAOQQY010000000
VERSION     DAOQQY010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10015_length_4188_cov_5.509799"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="PLE23_10515"
     CDS             <1..500
                     /locus_tag="PLE23_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83740.1"
                     /translation="TADSLFTFFKVPIMWSDTSQFSADTIYVQMAKKAIDRVFLKKNS
                     LIINSPDFIFFNQIKGRDITARFLSSQIHDMFVEGNSEALYYAQDDRDAYIGVNKAVS
                     STMNLLFENKKIKDIFFLTNPESKFIPMKKANHEALKLKGFNWAPEKRPRTLEDLFMI
                     KIKAQ"
     gene            730..2193
                     /locus_tag="PLE23_10520"
     CDS             730..2193
                     /locus_tag="PLE23_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HMX83741.1"
                     /translation="MEKLFANLQPAGIWQHFLQLNAIPRASKKEQQVIEFMAAFGKSL
                     GLETVIDHIGNVIIRKPATPGMESRMPVVLQGHLDMVHQKNAGTDFDFVTQGIKMVVE
                     GDWVKAEGTTLGADNGIGVAAIMTILASNDVAHPAIDALFTIDEETGMTGALELQGGM
                     LNGNILLNLDTEEDTELTIGCAGGIDVTAKGSYIDRPTKKNVKSYRLTVSGLTGGHSG
                     MDIHLGRGNANKIMNRLLLEIIEKTGAQIYSIDGGGLRNAIPRESVAEMAVSEDVVEI
                     FEALVKAEADCIIKEYRTTDAALDISLSTTAHQPVAIDSEKALALIRAVYTCPNGIYR
                     MSPDIEHLVQSSNNIARVIVRDGEYTVMCLTRSSVDTEKIDLANAIMCAFELVGANVE
                     FSGSYPGWTPEPNSSIVKLMRELYETMYHSKPNVNACHAGLECGIIGRNYPKMEMISF
                     GPNIRGAHSPDEKVQISSVQKFWDYLLATLKNIPLMQ"
     gene            2332..2877
                     /locus_tag="PLE23_10525"
     CDS             2332..2877
                     /locus_tag="PLE23_10525"
                     /inference="COORDINATES: protein motif:HMM:NF020369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83742.1"
                     /translation="MKTIQKLTLLLALLVMCTGVFAQDAKSTKDKKDKKEMTDSKGKK
                     EKKEKKDKKEKAETKDMTKKDESAPEKVKKEKKSKKEKADTKDMTKKDATSTATEKEA
                     KQASAKEAKAGKYAAKPAHEAKKSESKAKKAAPNVKVNDPVDRSLKGPSGQTIYTGPR
                     GGKYYINSNGNKTYLEGRAEK"
     gene            complement(2962..>4188)
                     /locus_tag="PLE23_10530"
     CDS             complement(2962..>4188)
                     /locus_tag="PLE23_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83743.1"
                     /translation="ATDPQSAGGTTQMKAKKVKNVVNVFLPQSFTKPGLLGYYTEGYP
                     GYQNDFLVVKKSQVGKGIYTIEHEIGHFFSLLHPFNGWEDNDYDPSVHGNPVKILTAP
                     NFSLDPPFNAVPVEFVDQTNCEIAGDLICDTPASYNFANNYWGAKGCQPMDQSVKDYH
                     GDVVNPDEENLMDYFNGCASYHLSPTQANLVKTDLTTRFATFGPQKLNGGYVPTADLV
                     TSKVTAPNPAHQQTISNFGDIKLDWEDVPGATHYFVMLDVTSSFSFKPVSQIVTKSEW
                     TATGLAKNKKYNWLVYPYNERSTCAGPAVFEFTTGSWAVSTTEIKELDKWTLQPNPAI
                     SGMDVMINATTIDKFNASVCVMDITGKILHTQSDVHFFAGDNTVKISTDNLASGMYIV
                     QIKSLSGVSTKKLVIE"
BASE COUNT         1305 a          812 c          905 g         1166 t
ORIGIN      
        1 atacagccga ttcgctgttt actttcttca aggtaccgat aatgtggagc gatacttccc
       61 aattctcagc cgataccatc tacgtacaga tggcaaaaaa ggcgatagat agggttttct
      121 tgaagaaaaa ttcgctcatc atcaattcgc ccgattttat atttttcaat cagattaaag
      181 gtcgggatat taccgcccga tttttatcaa gtcagataca cgatatgttt gtagaaggca
      241 attcggaggc actgtattat gcccaggatg accgtgatgc atatataggc gttaataaag
      301 cagtgagttc gaccatgaat ctgttatttg agaataaaaa aataaaagac attttcttcc
      361 tgaccaatcc ggaatcgaaa ttcatcccga tgaaaaaagc caatcatgaa gcattgaaac
      421 tcaagggttt taactgggca cctgagaaaa ggccccgtac gcttgaagat ttatttatga
      481 taaaaatcaa agcacagtaa aactgaaaaa acaatacagt attcatattt tatttcttct
      541 gccaggctga ttgttcgtta aaaacttcag ttacatcaat ttttttacac gaaagatgag
      601 cgttttaacc aaaaaagtct taattttatt tgaaattcag gtttggtagc ttttattagt
      661 ttttcagccg aaatgagctt ttgttttaag tacaagaata ttgttaatta atcatgtaag
      721 ataaatatta tggagaagct atttgcaaat ttacaaccgg caggcatttg gcaacatttt
      781 ttacaactca atgccattcc gagggcttca aaaaaagaac aacaggtcat cgaattcatg
      841 gcagcattcg gcaaatcgct tggtctggaa acagtcattg atcacatagg caatgttatc
      901 atccgcaagc cggcaacacc cggtatggaa tctcgaatgc ccgttgtact tcaggggcac
      961 ctcgatatgg ttcatcagaa aaatgcaggt acagatttcg attttgtaac ccaaggcatc
     1021 aagatggtgg tagaaggtga ctgggtaaag gctgagggta caacccttgg agctgataac
     1081 ggtatcggag ttgcagccat catgactatc cttgcatcaa atgatgtcgc gcatccggca
     1141 atcgatgctc tttttacaat tgatgaagaa accggtatga ccggagcact cgaactccaa
     1201 ggaggcatgc ttaatggaaa tattctcctc aacctcgaca ccgaagaaga cactgagctg
     1261 accatcggtt gtgccggagg tatagatgtg acagcaaaag gcagctacat tgatcgtccg
     1321 accaaaaaaa atgtgaagtc ttatcggcta actgtatcag ggcttaccgg tgggcattca
     1381 ggtatggata ttcacctggg gcgtggcaat gccaataaga ttatgaacag gcttcttctt
     1441 gaaataattg aaaaaaccgg tgcacagatt tacagtatcg atggcggtgg gctacgaaat
     1501 gctattccac gcgaatcggt agctgaaatg gctgtctcgg aagatgtggt cgaaattttc
     1561 gaagcacttg tgaaggctga agctgactgt attattaaag aatatcgcac tacagacgct
     1621 gctctcgata tcagcctttc aacaacagcg catcagccgg tagcgattga ttctgaaaag
     1681 gcacttgctc tcattcgcgc tgtatatacc tgcccaaatg gtatctaccg catgagtccc
     1741 gatatcgaac accttgtgca aagttcgaac aatattgcaa gagtaatcgt cagagacggt
     1801 gaatatacag tgatgtgtct gaccagaagt tcagttgata ccgaaaagat agaccttgcc
     1861 aatgctatta tgtgtgcttt tgagcttgta ggagccaatg tagagttttc aggttcatat
     1921 ccgggatgga cacctgagcc taattcttcg attgtcaaac tcatgaggga actttatgaa
     1981 accatgtatc actccaaacc gaatgtcaat gcctgtcatg cagggctcga atgtggtatt
     2041 atcggaagga attatccaaa aatggaaatg atttcgtttg gtccgaatat tcgcggggca
     2101 cattcgcctg acgaaaaggt acagatatct tctgttcaga aattttggga ttatctgctc
     2161 gctaccttaa aaaatattcc gctaatgcaa taggcagaag tttttgtgtg ttcagaaagt
     2221 gatatctatg atttaggaag ctgagttaat attaaagttg tgttaataca tgtatgtaaa
     2281 taccggccat gtagtacatt tgctcattat taatcaacta aataattttc catgaaaaca
     2341 attcaaaaac tgacactttt acttgcattg cttgtaatgt gtaccggtgt ttttgctcag
     2401 gatgccaaaa gcacaaaaga caaaaaagac aaaaaggaaa tgaccgattc gaagggcaaa
     2461 aaggaaaaga aagaaaaaaa ggataaaaag gaaaaagctg agacaaaaga catgactaaa
     2521 aaagatgagt ctgctcctga aaaagtcaaa aaagaaaaga aaagcaaaaa ggaaaaagcg
     2581 gataccaaag acatgactaa gaaagatgca acatcaactg ccactgaaaa ggaagctaag
     2641 caagcttctg ctaaggaagc taaggcagga aagtatgcgg caaagcctgc acacgaagca
     2701 aaaaaatctg aatcgaaagc caaaaaagct gcccctaatg taaaggtcaa cgacccggtt
     2761 gacagaagtc tgaaaggccc gagtggtcag accatttata ctggtcctcg cggtggcaag
     2821 tattacatca acagtaacgg taacaaaacg tatctcgaag gtagagccga gaaataaccc
     2881 tcatcacgta gggtttttac taaaaaagcc ggacttaatt aaaagttccg gcttttttaa
     2941 ttctgatttt tatttaaatg tctattcgat aacgagtttt ttggttgaaa caccagacag
     3001 agatttgatc tgtacgatat acattcctga agccagattg tctgttgaga tttttacagt
     3061 attatcgccg gcaaagaaat gcacatcact ttgtgtgtga agtatttttc ccgtaatatc
     3121 cattacacaa acactagcgt tgaatttgtc aattgtggtg gcatttatca ttacatccat
     3181 tccgctaatc gcaggatttg gctgtagtgt ccatttgtct agttctttga tttcggtggt
     3241 tgataccgcc cacgaacctg tagtgaattc aaaaacagcc ggacctgcac aagtgcttcg
     3301 ctcgttgtac ggatatacta accagttgta tttcttgttt tttgcaagac ctgtagctgt
     3361 ccattcgctc ttggttacta tctgcgaaac aggtttgaaa gaaaaagaag aagtaacatc
     3421 aagcatgaca aaatagtgtg ttgctccggg tacgtcttcc cagtcgagct ttatgtctcc
     3481 aaaattagag atggtctgtt ggtgtgcagg gttcggtgca gttacttttg aagtgacaag
     3541 gtcagcagta ggtacatagc ctccgttcaa tttttgtggg ccaaatgtag caaaacgagt
     3601 ggtaaggtcg gtttttacca gattggcctg tgtaggtgat aaatggtacg atgcacagcc
     3661 attgaaataa tccatcagat tttcttcatc cggattaact acatcaccat gataatcttt
     3721 gactgactga tccattggct gacaaccttt ggcgccccag taattattgg caaaattata
     3781 actggctgga gtatcacata tcaggtcacc tgctatttca caatttgtct gatctacgaa
     3841 ctcgaccggt acagcgttaa aaggtggatc aagtgaaaaa ttgggtgcag tcaatatctt
     3901 taccggattg ccatgtacgg aaggatcata atcattgtcc tcccaaccgt tgaatgggtg
     3961 cagcaggctg aaaaaatgcc cgatttcgtg ttcgatggta tagatacctt ttcctacctg
     4021 acttttcttt actaccagaa agtcattttg atagccgggg taaccttctg tgtaataccc
     4081 gagcaatccc ggttttgtaa aactctgtgg caggaaaaca ttgactacat tctttacctt
     4141 tttggctttc atctgagtgg tgccacctgc cgactgtggg tcggtagc
//
LOCUS       DAOQQY010000249         4176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10055_length_4176_cov_5.428537, whole genome shotgun sequence.
ACCESSION   DAOQQY010000249 DAOQQY010000000
VERSION     DAOQQY010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10055_length_4176_cov_5.428537"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLE23_10535"
     CDS             <1..296
                     /locus_tag="PLE23_10535"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HMX83744.1"
                     /translation="TYFFINNLKLNMTQTENTELLQMVEDALDVIRPHLLVDGGNVEV
                     IELTPDGVLKLKWLGNCESCSMSLFTMKAGIEQAIIGKVPFVKTVEAINGLNQ"
     gene            300..1103
                     /gene="pyrF"
                     /locus_tag="PLE23_10540"
     CDS             300..1103
                     /gene="pyrF"
                     /locus_tag="PLE23_10540"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMX83745.1"
                     /translation="MTRSELVEQIKARKSFLCVGLDTDMSKIPAILNGSDDPAFEFNK
                     KIIDATQDFCVAYKLNTAFYEANASAGWESLKKTIEYIPASHFVIADAKRGDIGNTST
                     MYANAFFENLHADAVTVAPYMGEDSVRPFLQFNGKWTILLALTSNEGSRDFQYFEIND
                     DLPLYKKVILKSQDWGTTENLMYVVGATRIEQLTEIRHIAPEHFLLIPGVGAQGGSLK
                     EVAEKTLIKDYGILINSSREIIYASSGEDFAEKAAEKAQTLQQEMLRYF"
     gene            complement(1112..2500)
                     /locus_tag="PLE23_10545"
     CDS             complement(1112..2500)
                     /locus_tag="PLE23_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HMX83746.1"
                     /translation="MKKNLRFAVIDIETTGGRADRDRITEIGIVITDGNTVFDSFESL
                     VNPEMGIPPYITQLTGIDNEMVEDAPRFYEIAKKIVELTEDTIFVAHNVRFDYGFIQE
                     EFRRLGYSFSKKKICTVVMSRKAFPGLKSYSLGNLIRYFNIPAERRHRALDDAQATST
                     LLSYILESQSTSNEIYRLVRDLHQESLLPAELPADKINALPEVCGVYYCYDKYGEIAY
                     IGKALNIRKRIWEHFKGIDNKGNKMQNTVADISFIETRSELAAYLTESFEIKKYQPYL
                     NKASRIDTKNIGVYMRYKPEIGAYFSIEKKSDESDPDYLHSFRTIRLATKTLNNLIRE
                     FGLCPDVSMGNILSQACFDSMIDLCAGVCCGKEEKEAHQKRLHAAALRIGQKLEGSWF
                     LVDEAPSYDEMTVFYIENGIYKGYCVVSNEENLLPEAIIDQIKTVSYHTDYQIIIKKY
                     FKKKSIRKIRLS"
     gene            2531..3736
                     /locus_tag="PLE23_10550"
     CDS             2531..3736
                     /locus_tag="PLE23_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HMX83747.1"
                     /translation="MLKFVHFFLLLAIFACKSSSEAQSQSKPATVERVIQPGAYMTAE
                     YFHLLDKKNVALVINQTSTIGNSSLADTLMSAGIKVRKIFAPEHGFRGKADAGAHISN
                     ETDLQTGIPVVSLYGSKKKPTPDDLSGIDVLVFDIQDVGVRFYTYISTLHYVMEACAE
                     QNIKLIVLDRPNPLGFYIDGPVLDKKFTSFVGMHSVPVVYGMTIGEYAAMIKGENWIK
                     KSEDLDLLVVRCKDYDHTSHYDIPVAPSPNLRTTRSILLYPSLCFFEGTIVSVGRGTD
                     YPFEVFGYPGGNGDFSFTPRPSEGASDPLYNGKRCVGYSLHNISLDKLYHTKQINLQH
                     LIKMYNSYKGKDFFLANHFFDKLAGTDQLKEQILSGMQESKIRESWQPEIEKFRKIRK
                     KYLLYKDFE"
     gene            complement(3744..>4176)
                     /locus_tag="PLE23_10555"
     CDS             complement(3744..>4176)
                     /locus_tag="PLE23_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMX83748.1"
                     /translation="IRVHLAHKLNKNLELRNRFEWVFYQDASGQKSNGFMVFQDVLYK
                     PIQIPVSFTARLAYFDTKDYSSRIYAFENDLLYSFSIPPYYYKGTRWYLNVRSKIARN
                     IMLEAGIAQTILANQKTIGSGLEQINGNTKTELRAQMKLSF"
BASE COUNT         1264 a          785 c          818 g         1309 t
ORIGIN      
        1 agacgtactt ttttataaat aatttgaaat taaatatgac ccaaactgaa aatactgagt
       61 tgctgcaaat ggtagaagat gctcttgatg tcataaggcc tcacttgttg gtagatggtg
      121 gaaatgttga ggtaattgag ctgacacctg atggcgtact caaattgaaa tggcttggca
      181 attgtgaatc atgcagtatg tcactattta ctatgaaagc cggcatcgag caagccatta
      241 tcggcaaggt gccatttgta aaaactgtag aagccatcaa cggattaaac cagtaatcaa
      301 tgactagaag tgaacttgta gaacaaatca aagccagaaa atcatttttg tgtgtcgggc
      361 ttgatacaga tatgtctaaa atacctgcaa tactaaatgg tagtgatgac cctgcctttg
      421 aattcaacaa gaaaattatt gatgcaacac aagatttttg cgttgcttat aagcttaata
      481 cagccttcta tgaagcaaac gcatccgccg gttgggaaag tctaaaaaaa acaattgaat
      541 acattccggc atctcatttt gtgattgcag atgccaaacg aggcgatatt ggcaacactt
      601 ctactatgta tgctaacgca ttttttgaaa atctacatgc cgatgccgtt accgttgccc
      661 catatatggg cgaagacagt gtcagaccat ttcttcaatt caacggcaaa tggactattc
      721 tactcgcact tacatcgaat gaaggaagcc gcgattttca gtattttgaa atcaatgatg
      781 atttaccgtt atataaaaaa gtaattctta aatcccaaga ttggggtaca accgaaaacc
      841 ttatgtacgt agtaggtgct acacggattg agcaacttac tgaaatccga catatagcac
      901 ctgagcattt tttactgata ccgggtgtag gtgcacaagg tggttcactc aaagaagtag
      961 ccgaaaaaac actgattaag gactatggga tattgattaa ttcgagtcgt gaaatcattt
     1021 acgcatcatc tggtgaagat tttgccgaaa aggctgccga gaaagcgcag acactccagc
     1081 aagaaatgct tcgttacttc taggttttta cttaactaag ccttatctta cgtattgact
     1141 ttttcttgaa atacttcttg ataattatct gataatctgt atgataagat acagttttaa
     1201 tttgatcaat gatggcctcc ggcaatagat tttcttcatt tgacacaaca caataacctt
     1261 tataaatgcc attttcgata tagaagactg tcatttcatc atacgacggt gcttcatcta
     1321 ctaaaaacca acttccttcg agtttctgac ctattcgcag tgcagcagca tgtagtcttt
     1381 tttgatgtgc ttctttttct tcttttccgc aacagacacc ggcacaaaga tcaatcatac
     1441 tgtcaaaaca agcttggctt agaatattac ccattgatac atccggacat agaccgaact
     1501 ctctaatcaa attgttgagt gttttggtgg caagccgaat ggttctgaaa gaatgaagat
     1561 aatctgggtc agactcgtct gatttttttt ctattgaaaa atatgctcca atctcaggtt
     1621 tgtacctcat atagacaccg atattcttag tgtcaatccg ggaggcttta ttcagataag
     1681 gttgatattt cttgatttca aatgattcgg taaggtaagc agccagttca gaacgagttt
     1741 ctatgaatga aatatctgct actgtgttct gcattttatt acccttgttg tcaatgcctt
     1801 taaaatgttc ccagatacgc tttctgatat ttagagcttt accaatgtat gcgatttcgc
     1861 catatttgtc atagcaataa tacacaccac aaacttccgg caatgcattg attttatccg
     1921 ccggtaattc ggcaggcaat aacgattcct gatggaggtc acgtacaagc cggtaaattt
     1981 catttgatgt tgattgtgat tcaagaatgt agctcagtag tgtacttgta gcttgtgcat
     2041 catctaatgc tctgtgcctt ctttcagcag gaatattaaa ataccgaatt aaattaccaa
     2101 gggaatacga tttgagaccg ggaaatgctt tgcgactcat gactacagta caaattttct
     2161 ttttgctaaa actgtacccg agcctgcgga attcctcctg aatgaatcca taatcaaatc
     2221 ggacattgtg cgctacaaat attgtatctt ccgttaattc aacgattttt ttagctattt
     2281 cataaaatct cggggcatct tcgaccattt cattatcgat acctgttagc tgggtaatat
     2341 aaggcggtat ccccatttca ggattaacca atgattcgaa tgaatcaaag actgtatttc
     2401 cgtcagttat gacaatgcca atttctgtaa ttcgatcgcg gtcggcacgt cctccggtag
     2461 tttcgatgtc aataactgcg aatcttaaat tttttttcat ttctttgcta cgacaaatct
     2521 aaagttattt atgttgaaat ttgttcattt ttttctattg ttggcgattt ttgcctgcaa
     2581 atcttcttca gaagcacaga gtcaatcaaa acctgcaact gtagaaagag ttattcaacc
     2641 gggtgcatac atgactgccg aatattttca tttgctcgat aaaaaaaatg ttgcgcttgt
     2701 cattaatcag acttcgacaa taggaaactc aagtttggct gatactttaa tgtcagcagg
     2761 tattaaagta aggaagattt ttgctcccga acacggattc agaggaaagg cagatgccgg
     2821 tgcacatata tcaaatgaaa cggatcttca aaccgggatt cctgttgttt ctctttacgg
     2881 ttcaaagaaa aagccaacac ccgatgattt aagtggtatt gatgtgcttg tgtttgacat
     2941 acaagatgtt ggcgtaaggt tctatacata tatttctacg ctgcattatg taatggaagc
     3001 ttgtgctgag caaaacataa agctaatagt gcttgacagg cctaacccac ttggtttcta
     3061 cattgacggc cccgtattgg ataaaaaatt tacgtcattt gtcggtatgc atagtgtgcc
     3121 ggtagtatat ggcatgacaa tcggtgaata tgccgcaatg ataaaaggtg aaaactggat
     3181 taaaaaatca gaggatttag atcttcttgt tgtaagatgc aaagattatg atcatacttc
     3241 tcattacgat attcctgtgg caccgtcacc caacctgcgt actacacgga gtattttgct
     3301 ttacccgagt ctttgttttt ttgaagggac catcgtaagc gtaggccggg gtacggatta
     3361 tccttttgaa gtattcggtt atccgggcgg aaatggtgat ttttctttca caccaagacc
     3421 ctctgaaggt gcaagtgatc ctttgtataa tggcaaacgg tgtgtcggtt atagtttaca
     3481 taatatatct ttggataagc tgtatcatac caaacagata aatctgcaac atctaataaa
     3541 aatgtacaat agctataaag gcaaggattt ttttcttgcc aaccatttct tcgacaaact
     3601 tgcaggtaca gaccagttaa aagaacagat actatcagga atgcaagaat caaagattag
     3661 ggaaagttgg cagccggaaa ttgaaaaatt cagaaaaatt cgcaaaaaat atttgttgta
     3721 caaagatttt gagtaactga ctatcagaac gaaagtttca tttgtgccct gagctctgtt
     3781 tttgtatttc cattgatctg ttcgagcccc gaaccaatag ttttctggtt ggcaaggata
     3841 gtttgtgcaa tacctgcctc tagcatgata ttccttgcta ttttgcttct tacattcaga
     3901 taccagcggg tacctttgta gtaatagggc ggaatcgaaa aactatacag aaggtcattt
     3961 tcgaaagcat aaatacgact actgtaatct ttggtgtcaa aataagctaa acgtgccgta
     4021 aaactcacag gtatctggat gggtttgtac aacacatcct gaaaaaccat gaagccgtta
     4081 gatttttggc cggaagcatc ttggtaaaaa acccattcaa aacgatttcg tagttctagg
     4141 tttttgttga gtttgtgtgc caggtgaact cttatc
//
LOCUS       DAOQQY010000250         4159 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10115_length_4159_cov_5.176413, whole genome shotgun sequence.
ACCESSION   DAOQQY010000250 DAOQQY010000000
VERSION     DAOQQY010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10115_length_4159_cov_5.176413"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLE23_10560"
     CDS             complement(<1..215)
                     /locus_tag="PLE23_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMX83749.1"
                     /translation="MVDNSVSEFFRQKNIIFFKTLDSTQSYALELTSKSKPIDKTAIL
                     TYNQSAGKGQLGAKWQTQADKNLCMSI"
     gene            270..650
                     /gene="rsfS"
                     /locus_tag="PLE23_10565"
     CDS             270..650
                     /gene="rsfS"
                     /locus_tag="PLE23_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMX83750.1"
                     /translation="MTTATETTILQEEQLNDLIVDAIQDIKAKNITKFDLRHLHDRPT
                     NYFIICEGESNTQVNAIIDRVRQRVKEEKGILPHTVEGKDSARWVLADYFTTVVHVFY
                     KETRAYYDLDDLWSDAKITHYEDL"
     gene            697..2697
                     /gene="ftsH"
                     /locus_tag="PLE23_10570"
     CDS             697..2697
                     /gene="ftsH"
                     /locus_tag="PLE23_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMX83751.1"
                     /translation="MENKENNNNNNKPRKSGGFRMGSYWIYGVIFLALITINILTVSN
                     GKSKEITFNRLESLIRAGHIDKIEVINEKVGQIYLNKDSLSRYKETKYSDVVDEKLFG
                     ESQANYVMNIGPSASFDEKINAINQTLPPNIAKVDIQYVTQQNWWGPIFSWLLLPLLF
                     IGLWIMIMRRVGGGGAGPGAQIFNIGKSKASLFDANAKVDVTFKDVAGLDEAKEEVME
                     VVDFLKNPKKYTALGGKIPKGVLLVGPPGTGKTLLAKAVAGEAGVPFFSLSGSDFVEM
                     FVGVGASRVRDLFKNAREKAPCIIFIDEIDAIGRARGKGAMQGGNDERENTLNQLLVE
                     MDGFSTDKGVIIMAATNRPDVLDSALLRPGRFDRQIGIDPPDLIGREQIFKVHLKNIK
                     LSLDVKPSALAEMTPGFAGADIANVCNEAALIAARKNKQAVDMDDFNQALDRVIGGLE
                     KKNKLIHKSEKEIIAYHEAGHAICGWFLEHASPLVKVTIVPRGIGTLGYAQYLPKEEY
                     ITRTEQLLDRICMTFGGRAAESIVFGKISTGAQSDLDQITKMAYGMVAVYGMNNSVGN
                     VSFYGLQQDQFQKPYSEQTAAMIDQEVRELVAKQYERAKALLTERRHELEVIAHHLLE
                     KEVLLKSDLEELIGPRPFVDPDKDQHSGHQAEEPVADNQPVQ"
     gene            complement(2851..3603)
                     /locus_tag="PLE23_10575"
     CDS             complement(2851..3603)
                     /locus_tag="PLE23_10575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX83752.1"
                     /translation="MKLSIITSCYNSAATIRDTLESVARQDYSDIEHIIVDGGSEDDT
                     LAIVSEFTHVAKVVSEKDRGIYDAMNKGIAIATGDIIGILNSDDFYTGPGVISDVVRQ
                     MEQSGADTLYADLDYVSSTDKTKVVRSWRAGSYAVRKFYYGWMPPHPTFFVRKYIYER
                     YGTFNLSLRSAADYEIMLRFLVRQDVTTTYLNKTIVQMRAGGQSNASITNRLRANAED
                     RKAWQINKLRPYLFTTFLKPVRKIHQFFMRGK"
     gene            complement(3607..>4159)
                     /locus_tag="PLE23_10580"
     CDS             complement(3607..>4159)
                     /locus_tag="PLE23_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HMX83753.1"
                     /translation="NRMPDEQLYQAVTEQLVNGGVVGWFTGRAEFGPRALGGRSILAD
                     PRRNDVKEILNLKIKRRESFRPFAPSILKEYVDEYFEVNDVVPFMEKVFPIRPEKHAA
                     IPAVTHVDGSGRLQTVDRTVSERYYHLIDTFRQKTGVPILVNTSFNENEPIVNSPEDA
                     LECYLRTEMDMLVLENCVISRNF"
BASE COUNT         1176 a          983 c          945 g         1055 t
ORIGIN      
        1 attatactca tacagagatt cttgtctgct tgtgtctgcc attttgcccc caattgaccc
       61 ttgcctgccg actggttata cgtcaaaatg gcagttttat caattggttt gctttttgaa
      121 gtgagttcaa gtgcatacga ttgggtagaa tcgagcgttt taaagaatat aatatttttt
      181 tgcctgaaaa actctgaaac actgttatcc accatcgtta taactatatt tgtactaaga
      241 tttacaaaat tacttaaaaa catcaaaatt tgactactgc caccgaaaca actattcttc
      301 aggaagaaca attaaatgat ctgatagtgg atgctatcca ggacatcaaa gctaagaata
      361 ttacaaaatt cgatcttcga catttgcacg accggcctac caattatttc attatatgcg
      421 aaggtgaatc aaacactcag gtgaatgcta tcattgatcg ggtaaggcaa cgggtcaagg
      481 aagaaaaagg tatcctaccg cataccgtgg aaggaaaaga tagtgccagg tgggtactcg
      541 ccgattactt tactacagtt gttcatgtat tttataaaga aacacgagcc tactacgacc
      601 tcgatgactt gtggtctgat gccaaaatta ctcattacga agatctttaa tgaacgtttt
      661 caaaatataa acgttcgtat ataaccaatc tataaaatgg agaataaaga aaataacaat
      721 aataacaata aacctcgaaa atccggaggt ttcagaatgg gttcgtattg gatctatgga
      781 gtgatttttc tggcattgat aacaatcaat atcctgaccg tttcaaacgg taaatcgaaa
      841 gaaatcacct tcaaccgact agaatcactc atccgtgccg gtcacatcga caagatagaa
      901 gttatcaacg aaaaagttgg acagatatac ctcaacaaag actctctgag caggtataag
      961 gagacaaaat atagcgatgt agtagatgaa aagctcttcg gcgaatcgca ggccaactat
     1021 gtcatgaaca ttggcccttc tgcctctttt gatgaaaaaa taaatgccat caaccaaacg
     1081 ttgccaccca atattgccaa agttgacatc cagtatgtca cacagcaaaa ctggtgggga
     1141 cctatattca gctggctttt actcccattg cttttcattg gcctatggat catgattatg
     1201 cgccgtgtag gcggaggtgg tgccggaccg ggtgctcaaa tctttaacat tggtaagtct
     1261 aaggcctcac tgtttgatgc caacgccaag gtagatgtca ccttcaaaga tgtagcaggt
     1321 ctcgacgaag caaaggaaga agtaatggaa gtagtagatt tcctaaaaaa tcctaaaaaa
     1381 tatactgcac tcggtggcaa aataccaaaa ggggtactcc tcgtaggccc tccgggtacc
     1441 ggcaaaaccc tgcttgccaa agctgtagca ggtgaagcag gcgtaccttt cttttcatta
     1501 tcgggctccg actttgtcga aatgttcgtt ggtgttggtg catctcgtgt gcgcgacttg
     1561 ttcaaaaacg cccgcgaaaa agcaccctgc atcattttca ttgacgaaat cgatgccata
     1621 ggccgtgctc ggggcaaagg tgctatgcaa ggtggtaatg acgaacgcga aaacacactc
     1681 aaccaactcc tcgtagagat ggatggcttc agtaccgata aaggcgtcat cattatggct
     1741 gccaccaaca gacccgatgt actcgacagt gccttgcttc gccctggccg cttcgacaga
     1801 cagataggca tagacccacc tgacctcatc ggtcgcgaac agatatttaa agtgcacctc
     1861 aaaaacatca aactatcact cgatgtcaag ccatcggctt tggctgagat gacacccggt
     1921 ttcgcaggtg ccgacatcgc caacgtttgc aacgaagcag cactcatcgc cgcacgtaaa
     1981 aacaaacagg cagtcgatat ggacgacttt aaccaggctc tcgaccgcgt tatcggtggc
     2041 ctcgaaaaga aaaacaaact catccataag tccgaaaagg aaatcatcgc ctaccatgaa
     2101 gccgggcacg ccatctgtgg ctggtttcta gagcacgcaa gcccacttgt caaggtcacc
     2161 atcgttccac gaggcattgg tacacttggc tatgctcagt accttccaaa agaagaatac
     2221 attacccgta ccgagcaact cctcgaccgt atctgtatga ccttcggagg tcgcgcagca
     2281 gaatctatcg tattcggaaa aatatctacc ggcgcacagt ccgacctgga tcagattacc
     2341 aagatggcat acggtatggt tgccgtttat ggtatgaaca acagcgtcgg caacgtttca
     2401 ttctacggcc tgcagcaaga ccagttccaa aaaccatact cagagcagac tgctgccatg
     2461 atagaccagg aagtacgcga actcgtagcc aaacaatacg aaagagccaa agccctgctt
     2521 acagaacgac gtcacgaact tgaagtcata gcccaccacc tgcttgagaa agaagttctc
     2581 ctcaagtcag accttgaaga gctcatcggc ccgcgccctt ttgttgaccc cgataaagat
     2641 cagcactccg gccatcaggc agaagaaccg gtagccgaca atcagcctgt acaatagcct
     2701 taaccgagca ccatatacaa cacaaaaagg gtttactcat ctgcagtaaa ccctttttta
     2761 catccaataa tgcacgcagt ttgggcctac ccttgtcaag ggcagcctga tgcacttaaa
     2821 aataatctgt gaaaagtagc cgtatttgtg ttatttgcca cgcataaaaa actggtgaat
     2881 cttgcgtacc ggcttgagaa aggtcgtaaa aaggtaaggg cggagtttgt taatctgcca
     2941 tgccttgcga tcttcggcat tggcccttag tcggttagtg atagaagcat tgctctgacc
     3001 accggcgcgc atctgtacga tcgtcttatt gaggtaagtg gtcgtaacat cctgcctgac
     3061 cagaaagcgg agcattatct cgtagtcggc tgctgatcta agacttagat taaatgtacc
     3121 atagcgttca taaatatatt tcctgacaaa gaaagtaggg tgtggcggca tccagccgta
     3181 gtaaaacttc ctgactgcat agctgcctgc acgccaacta cgcactactt ttgttttgtc
     3241 ggtagaagaa acgtaatcga ggtcggcata aagtgtgtcg gcaccgctct gctccatttg
     3301 ccgtaccaca tctgatatga cacccggtcc ggtataaaaa tcatccgaat tgaggatgcc
     3361 gatgatgtcg ccggtggcta tggcaatgcc tttgttcatg gcgtcgtata taccacggtc
     3421 tttttcggac accaccttcg ccacatgagt gaattcactg acgatagcca aggtatcgtc
     3481 ttccgatccg ccgtcaacga tgatgtgctc gatatcgctg tagtcctgcc gggcaacgct
     3541 ctcaagtgta tcgcgtatgg tagcagcact gttatagcaa gaggtgatga tagagagttt
     3601 catttatcag aaattccttg atataacgca gttttcgagt accagcatat ccatttcggt
     3661 gcgaaggtag cattcgagag catcttcggg actgttgacg atgggttcgt tctcattaaa
     3721 ggatgtgttg acgaggatgg gtacgccggt tttttgtctg aacgtatcga tgaggtggta
     3781 atatctctcg gatactgtcc ggtcaacggt ttgcaggcga cctgagccgt caacgtgtgt
     3841 gactgcagga atagcggcat gcttttccgg tcggatcggg aatacttttt ccatgaaagg
     3901 cacgacatcg ttgacttcaa aatattcgtc cacgtattct ttgaggatgg acggtgcaaa
     3961 gggccggaag ctttcgcggc gtttgatttt gaggttcagg atctctttga cgtcgttgcg
     4021 ccttgggtca gcaaggatgg atctgccacc taatgctctt gggccgaact ctgccctgcc
     4081 ggtgaaccaa ccgaccacgc caccattgac gagctgttcg gtgactgcct gatacagctg
     4141 ctcgtcgggc attctgtta
//
LOCUS       DAOQQY010000251         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10127_length_4156_cov_5.028042, whole genome shotgun sequence.
ACCESSION   DAOQQY010000251 DAOQQY010000000
VERSION     DAOQQY010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10127_length_4156_cov_5.028042"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1625)
                     /locus_tag="PLE23_10585"
     CDS             complement(75..1625)
                     /locus_tag="PLE23_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83754.1"
                     /translation="MKNFIKNMALVFTFVAGAAASVWAQNDDLYYDPEKDAVSSQDVY
                     YDDVNNGNKDEASVARQNNNNSRPDGSSDNYKDYSDYDDDDYEYYYSSRIKRFHRPYS
                     GFGYFDDCYVNMYNYDPYWTGATIYIGYDSYSDYFRWRRYQRYHNWSHWGPTYTWGWG
                     YNPWGWNSWNSWGSPWTPWGSYAYSPCPPYGNIYINNVYYGNNWGHGGWYNNGWQGYY
                     DGGHYNEYTSNIHYGPRKTGGGISKLVDNPRGKTGLAIDNPRGVTNQGPTIENPGKVN
                     RNNGTTATPGQTTSRPDRTGNTGQIQSTKNDPIRGQAPQQNTKTEEQVKFNRRDNQQT
                     EEQVKYDRRDNGNTQQNTNPANPRDNQKPVRQDSPVQSPVKDNAPVQKPGRNNSPVQD
                     VKNDTEYKFNSRPSSAADRNTGEGYNNTSRPERGNINVPVERPQRQERVERPQRQERV
                     ERPQRQERVERPQRQETPAPRPSFRQESKPRLESPSPAPRQSQPSAPSRGNNSGDNNG
                     RSGNPRGR"
     gene            1861..3330
                     /gene="proS"
                     /locus_tag="PLE23_10590"
     CDS             1861..3330
                     /gene="proS"
                     /locus_tag="PLE23_10590"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMX83755.1"
                     /translation="MAKEITARSEDYSKWYNDLILKAGLADYSAVKGCMVIKPYGFAI
                     WELMRDQLDKMFKETGHVNAYFPLFVPKRLFEAEEKNAEGFAKECAVVTHYRLKTDPD
                     KPGKLMVDPEAKLEEELIVRPTSEAIIWNTYRDWIHSYRDLPLLINQWANVVRWEMRT
                     RLFLRTAEFLWQEGHTAHATATEAIEETVKMLNVYSDFVEGWMALPVVKGVKTPGERF
                     AGADDTYTIEALMQDGKALQSGTSHFLGQNFAKAFEVKFLNKENTQEYVWATSWGVST
                     RLMGALIMAHSDDEGLVLPPKLAPHQVVLVPVPFVNDELLNVMKDLEAELKQEGIRVK
                     ADSDETKRPGFKFAEYEMLGVPVRIAIGMRDLEKGMLEVARRDTKEKNLVPREGIASY
                     VKALLEEIQSNLFTRAKSFQQNNITIVNNWDEFKDVIENKGGFAYAHYDGTQETEDKI
                     KELTQATARCIPLDGEKESGVCILTGKPSAQRVLFAKAY"
     gene            3333..3890
                     /locus_tag="PLE23_10595"
     CDS             3333..3890
                     /locus_tag="PLE23_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83756.1"
                     /translation="MKRVLFFLLLSVSGLSVLEAQYLSGFSARYDDDLSEWLVYPESE
                     DFPECNIRCRWQIRQDPGEWDFRMGDTFGTIRTKWRDRYDDWEVIGDNQITRIRMVWP
                     GDPREWRIESSAGEYVIQMEYNQPGSDWIVEKDRKVIFRCRTVYQNDIRDWITEDDLP
                     EDEHFNTRVGLVFAVIISNVLAGMR"
BASE COUNT         1159 a          891 c          909 g         1197 t
ORIGIN      
        1 aacaaaatgg gtggaattca aagtttgtcc tccacccatt gcctcactat ttactcagag
       61 ttgtaactaa ttgcttacct gcctcttggg ttgcctgacc gaccattgtt gtcacccgaa
      121 ttatttcctc ttgaaggtgc actcggctgg ctttgtctag gagcaggtga cggactttcc
      181 agtctcggct tgctctcctg cctaaatgaa ggtcgcggtg caggtgtttc ctggcgctgt
      241 ggtctttcta ctctttcttg gcgctgtggt ctttctactc tttcctggcg ctgtggtctt
      301 tctactcttt cctggcgctg tggtctttct acaggtacat ttatattacc gcgttcaggc
      361 cttgaagtgt tgttataccc ttcaccggta tttctatcgg cagcactcga tggtcttgag
      421 ttaaatttgt attcagtatc attttttaca tcctgtacag ggctgttgtt tctacctggc
      481 ttctgtaccg gggcattgtc ctttaccggg ctttgtacag gactgtcctg tcttaccggt
      541 ttttggttat ccctcggatt agcagggttg gtattttgct gggtatttcc attatcgcgt
      601 ctgtcatatt ttacctgttc ttcggtttgc tgattgtcgc gacggttaaa tttcacctgc
      661 tcttcggtct tggtattctg ctgcggggcc tgacctctta tcgggtcgtt tttagtactt
      721 tgtatctgac ctgtattgcc ggtgcggtca ggtctagaag ttgtctgacc tggtgttgcg
      781 gttgtaccat tatttctgtt taccttacct gggttttcta tggtaggacc ttggttggta
      841 acacctcttg gattatcaat agccagacct gttttgcccc ttgggttgtc aacaagcttg
      901 ctgatacctc caccggtttt gcgtggacca tagtgtatat tacttgtata ttcattgtag
      961 tgtccgccgt cataataacc ttgccagcca ttgttgtacc agccaccgtg gccccagtta
     1021 ttgccataat aaacattatt tatataaata ttaccataag gcggacatgg tgaataggcg
     1081 tatgagcccc acggtgtcca aggactaccc catgagttcc agctattcca gccccatgga
     1141 ttgtatcccc aaccccaagt ataggtaggc ccccaatggc tccagttatg gtatctttgg
     1201 tatctacgcc atctgaaata gtcggaataa ctatcataac ctatataaat agttgcacct
     1261 gtccaatatg ggtcatagtt atacatattt acataacaat catcaaagta accgaagcct
     1321 gaataaggcc tgtgaaatct tttgattctt gacgaataat aatattcata atcatcatca
     1381 tcataatcgc tataatcttt gtaattatca gaacttccgt caggtctgct attattattg
     1441 ttttgtctgg caacagaagc ctcatccttg ttgccattgt taacatcatc ataataaaca
     1501 tcctgtgaag aaacagcatc cttctcagga tcgtaataaa ggtcatcatt ttgtgcccag
     1561 acagatgctg ctgcacccgc cacaaacgta aagaccaaag ccatgttttt aataaaattc
     1621 ttcataagac agagatttta gcgttatttt aatttattgc ttaaagtatt aggtcaaaaa
     1681 tattactttt ggccgattgt tttagttaaa taattaataa ggtttttttt caaatagtta
     1741 aatgttcaaa tcctcatctg ttggaaagac aattttcaag tattattagt tacatgaggt
     1801 atggtaattt aacatttttt tgactaaatc gtataagata tttcaaaaat aataaatatt
     1861 atggcaaaag agatcacagc acgaagtgaa gactacagca aatggtataa cgatctcata
     1921 ctcaaggccg gtctggccga ttattcggcg gtcaaaggtt gtatggtcat caaaccttat
     1981 ggtttcgcta tctgggaact gatgcgcgac cagctggata aaatgtttaa ggaaaccgga
     2041 cacgtaaatg cctatttccc actgtttgta cccaaaaggc tattcgaggc tgaagaaaaa
     2101 aatgctgaag gctttgcaaa agaatgtgcc gttgttacac attacagact caagaccgac
     2161 cccgataaac caggcaaact aatggtggac cctgaagcta agctcgagga agaactcatt
     2221 gtacgcccta cttctgaagc catcatctgg aacacctacc gcgactggat acacagctat
     2281 cgcgacttgc cattgctcat caaccagtgg gctaatgtag tacgatggga aatgcgtaca
     2341 cgcttgttcc tccgcacagc agaattccta tggcaggaag gacatactgc acacgctact
     2401 gccactgaag caatcgaaga aactgtaaaa atgctgaatg tatatagcga ttttgtagaa
     2461 ggctggatgg cactacctgt ggtaaaaggc gtaaaaacac ccggtgagcg ttttgccggt
     2521 gctgatgaca cctacaccat tgaagcactt atgcaggatg gcaaggcact gcagtcaggc
     2581 acatcgcatt tcctcggaca gaatttcgcc aaagccttcg aagtcaaatt cctcaataaa
     2641 gaaaatactc aggaatatgt ttgggcaaca tcctggggtg tatctacacg tctgatgggt
     2701 gccctcatca tggcgcattc tgacgacgaa gggcttgtac tgccaccaaa gctcgcaccg
     2761 catcaggtag tactcgtacc agtacctttc gtcaatgacg aattgctcaa cgtgatgaaa
     2821 gatctcgaag ctgaactgaa gcaagaaggt atccgtgtaa aagcggatag cgatgagaca
     2881 aagcgtccag gtttcaaatt cgctgaatac gaaatgctgg gtgtacctgt aagaatagcc
     2941 atcggtatgc gcgatctgga aaaaggaatg ctcgaagtag cccgccgcga cacaaaggag
     3001 aaaaacctgg taccaagaga aggcatagca tcttacgtaa aggcactttt ggaagaaatt
     3061 cagtccaacc tcttcacaag agcaaagtct tttcagcaaa ataatattac cattgtcaat
     3121 aattgggacg agtttaaaga tgttatcgag aacaaaggcg gttttgccta tgctcactat
     3181 gacggcaccc aggaaacaga agacaaaatc aaagagctca cacaggccac tgcaagatgt
     3241 attccactcg atggcgaaaa agaatccggt gtttgcatcc tgacagggaa accaagtgct
     3301 caacgggtgt tgtttgccaa agcgtattaa tcatgaaacg cgtgctgttc ttcttgctat
     3361 tgtctgtatc cggcttatcg gtgctggagg cacaatattt gtccggcttc agtgcaaggt
     3421 acgatgatga cctttccgaa tggcttgttt atccggagtc ggaagatttt ccggaatgca
     3481 acatcagatg cagatggcag atcaggcaag atccgggtga gtgggatttc aggatgggcg
     3541 acacctttgg caccatacgc accaaatggc gcgaccggta tgacgattgg gaggtgatag
     3601 gcgacaacca gataacccgg ataagaatgg tgtggcccgg tgaccctaga gaatggcgca
     3661 tagaatcttc tgcaggtgaa tatgtcatac agatggaata caaccaaccc ggaagcgact
     3721 ggattgtgga aaaagaccgg aaagtaatat tcaggtgccg tacggtttat caaaatgata
     3781 tcagagactg gataactgaa gacgacctac ccgaagacga gcatttcaat accagagttg
     3841 ggctggtatt cgctgttatc atttcgaatg tactggcagg catgcgttaa accccgatta
     3901 ttttgaaaac agaatattct acatctattt taaaaaaata ttcctgtaaa ttttgtaatt
     3961 ttaatttgta ttaaaaaaaa taaggaaata aattgcaata attaatatgc tatacttatt
     4021 tttgatgcaa ataatcaatc atgaaacgga tagttgtact gttagtcgct ttactcaatg
     4081 tagctaccat aggaaatcta tcagcccaaa gggtcggcca gaccaaaaaa acacaaaccg
     4141 gtaataaagc actagg
//
LOCUS       DAOQQY010000252         4142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10186_length_4142_cov_5.237827, whole genome shotgun sequence.
ACCESSION   DAOQQY010000252 DAOQQY010000000
VERSION     DAOQQY010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10186_length_4142_cov_5.237827"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /gene="queD"
                     /locus_tag="PLE23_10600"
     CDS             complement(<1..349)
                     /gene="queD"
                     /locus_tag="PLE23_10600"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HMX83757.1"
                     /translation="MEIFKQFSFDSAHFLPNVDENHKCRETHGHTYRLTVFIEGELDP
                     HLGWVTDFGDVKKVIDPVIKSIDHKLLNNIEGLENPTCERIAIWIWDKIKPGLPGLCR
                     IELHETLTSGVVYK"
     gene            complement(415..642)
                     /locus_tag="PLE23_10605"
     CDS             complement(415..642)
                     /locus_tag="PLE23_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HMX83758.1"
                     /translation="MVRIIQQRDKCIGCNACVEAAVYRWRLSRKDGKCTLVGGVSKKG
                     FYQVLVDDHELEENQRAAANCPVRIIQIIKV"
     gene            complement(645..1889)
                     /locus_tag="PLE23_10610"
     CDS             complement(645..1889)
                     /locus_tag="PLE23_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HMX83759.1"
                     /translation="MSNIKSGIELMAPAGSFESMMAAIKAGCNSVYFGVEQLNMRARS
                     SNNFTLEDLKEIARIGKENNVKTYLTLNTILYDHDIKLMKNIVDAVKENGITAIIAAD
                     HAVMNYAKKIGVELHISTQANISNIDTIEFYANFADVVVMARELSLMQVADITREIKR
                     RNVTGPSGKLVEVEIFAHGALCMAVSGKCYMSLHSHYASANRGACIQNCRRSYVVTDK
                     DEGIEFEVDNEYIMSAKDLSTIGFLDQILDAGVSVLKLEGRGRSADYVYTVTKCYREA
                     IDAYKDGSYSRENVANWEARLATVFNRGFWDGYYLGRKMGEWNDVYGSKATKRKIYIG
                     KGIKFFEKASVAEFKLETHSLSVGDEIMVTGPTTGYIEAKVSEIRINDQPVEKVNRGE
                     QFSIHLSEKIRPSDKLYKVVDA"
     gene            complement(2042..2920)
                     /locus_tag="PLE23_10615"
     CDS             complement(2042..2920)
                     /locus_tag="PLE23_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMX83760.1"
                     /translation="MNALFDWLKYLEHIVLFKLGEHSFTVGTLFYLVLSLWILFYLTG
                     LLKKLLINKIFSKFHMDFGVSQAIATLIRYFLVMIGAIIIIQTSGIDLSTVGLLIGGL
                     GIGIGLGLQNIVTNLISGIIILFERPIKVGDRVEVGGILGTVKKISPRSTTILTNDNV
                     AIIVPNAEFMTTHVINWSHNDDKIRFHVSVNIHFETDFNLVSNLLLEIASENQDVLDD
                     PKPDVRLEEFGDHGIRCTLLVWSSSLLHRQGKLKSNINKAIYKKFKEHGIEIPYAQRE
                     LRLKSRPGDDFQINSD"
     gene            complement(2964..3440)
                     /locus_tag="PLE23_10620"
     CDS             complement(2964..3440)
                     /locus_tag="PLE23_10620"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMX83761.1"
                     /translation="MAQKDEEFIRYVINLAADNVLKHGNGPFAAIVVKDGKIIGEGNN
                     SVTNNFDPTAHAEVVAIRNACEHLKFHQLDDCVLYTSCEPCPMCFGAIYWARPERVVY
                     AATKKDAAEVGFDDAFIYEELKVGQSERRIEFTEINIPEKLLPFVKWAEKPDKILY"
     gene            3539..>4142
                     /locus_tag="PLE23_10625"
     CDS             3539..>4142
                     /locus_tag="PLE23_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMX83762.1"
                     /translation="MENKIDIEVSKNEDSLKLEVSRIKRLMQVIEKGGGEKRLEKIRS
                     QGKMTARERIEYLLDKDREYIEIGGFAGYGMYEEGGGCPAGGVVIFLGYIKGRLCIVV
                     ANDASVKAGAWFPITGKKNLRAQEIAMENRIPIIYLVDSAGVFLPMQDEIFPDKEHFG
                     RIFRNNARMSSMGIPQIAAIMGSCVAGGAYLPIMSDEALIV"
BASE COUNT         1165 a          919 c          816 g         1242 t
ORIGIN      
        1 ccttatatac cacacccgaa gtaagtgtct cgtgcaactc tattcggcaa agaccgggaa
       61 gaccgggttt tatcttgtcc cagatccaga tggcgatgcg ttcgcaggta gggttttcta
      121 agccttcgat gttattgagc agcttatggt cgatgctttt gatgaccggg tcaataacct
      181 tttttacatc accaaaatca gttacccaac caagatgcgg gtcgagttcg ccttcgatga
      241 atactgtaag cctataggtg tgcccgtgtg tttccctgca cttatgattt tcatcaacgt
      301 ttggcaaaaa atgtgctgag tcgaatgaga actgcttgaa tatttccata aaaacctgct
      361 gttgtcgtta tttttattaa tgaatagctt gtgcaatcaa tattatctgc aaagctacac
      421 ttttattatc tgaatgatgc gtaccggaca gttggcggca gcccgctgat tttcctccaa
      481 ctcatgatca tctaccagta cctgataaaa tccctttttt gaaacgccac ctacaagtgt
      541 acactttccg tcttttctcg acaaacgcca acgatacaca gccgcttcca cacaggcatt
      601 gcaaccgata catttatccc tttgttgtat tatccttacc atactcaggc atctactact
      661 ttataaagct tatccgatgg tcggattttt tcgcttaaat gtatgctgaa ctgctcgcct
      721 cggttcactt tttcgaccgg ctgatcgttg atgcgtatct cagaaacttt agcttcaata
      781 taacccgttg tagggccagt gaccataatc tcatctccta ctgacagact gtgtgtctct
      841 agtttgaatt ctgcaaccga tgctttttcg aagaatttta ttcctttgcc aatataaatt
      901 ttcctctttg ttgctttcga accgtaaacg tcattccact cacccatctt ccgacctaga
      961 tagtatccgt cccagaaacc gcggttgaac acagtagcca gccgtgcttc ccaatttgcg
     1021 acattttccc gcgaatagct tccgtcctta taggcatcaa tggcttcgcg gtagcatttg
     1081 gtaaccgtat agacataatc ggcagaacgg cctcgtcctt ccaattttaa tacagataca
     1141 ccggcatcca gaatctgatc gaggaatccg attgtgctga gatctttggc agacataatg
     1201 tattcgttgt caacttcaaa ctctatgcct tcatccttgt cggttacaac ataactcctg
     1261 cggcaattct gtatgcacgc tccacggttg gcagaagcgt aatgcgaatg cagactcata
     1321 taacatttgc ccgaaactgc catacataaa gctccgtgtg cgaatatctc cacttctacc
     1381 aattttccag acggtcctgt gacgttccgg cgttttattt cacgtgttat atcggccacc
     1441 tgcatcaggc ttagttcgcg tgccataacg actacatcag cgaagttggc ataaaactct
     1501 atggtatcga tgttcgagat gttggcctgt gtggaaatat gaagctctac gccgatcttc
     1561 tttgcgtaat tcatcacagc gtggtcagct gctatgatgg ctgtaatgcc attttccttc
     1621 acagcatcga ctatattttt cataagcttg atgtcgtggt cgtacaagat ggtattgagt
     1681 gtcaggtatg tttttacgtt gttttccttc cctatgcggg caatttcctt cagatcttca
     1741 agagtaaaat tattgctcga cctcgcacgc atattaagct gctcaacgcc aaaatatact
     1801 gagttgcatc ctgccttaat ggcagccatc atcgactcaa acgatccggc gggtgccatc
     1861 agttctattc cggattttat gtttgacata gtttggatat tgtattttgc aatattttga
     1921 aaattcaggc tgcaaaatta agattcccaa agggatataa caaatttaac caacaggcaa
     1981 tggcttcaat tgacaataaa aagacaaaaa catcttaaat aaccacgtat ttattaaaaa
     2041 actaatcact gtttatttga aaatcatcac caggcctaga tttaagcctt agctcgcgtt
     2101 gagcataagg tatctcaatg ccatgctctt tgaatttttt gtaaattgct ttattgatgt
     2161 tgctttttag tttgccctgg cggtgaagca acgagctcga ccacacaagc aaagtgcagc
     2221 gtattccatg atcaccgaat tcttcaagcc gtacatcggg ttttgggtcg tccagcacat
     2281 cttgattttc acttgctatc tcaagcaaca aattgctcac caggttaaaa tcggtttcga
     2341 aatggatgtt gaccgaaaca tgaaatctaa ttttgtcatc gttatggctc cagttaatta
     2401 catgcgtggt catgaattcg gcattcggta caatgatggc tacattgtca ttggtaagaa
     2461 tggtagtaga tcgcggcgaa attttcttta ctgtaccgag tattcctcct acttctaccc
     2521 tatcacctac ttttattgga cgctcaaata atataatgat accactgatt aggttagtga
     2581 ctatattctg caaaccaagc ccaataccga taccaagacc accaataagc aagccgaccg
     2641 tactgaggtc aatcccggaa gtctgaatga tgataatggc acctatcatc accagaaaat
     2701 atctgatgag tgtagcaatt gcctgactta ctccaaaatc catgtggaac tttgaaaata
     2761 ttttgttaat cagcaatttt ttcaacaaac cagtcaggta gaacagtatc cacagtgaaa
     2821 gcactagata aaacaaagtt ccaacagtga aggaatgttc tccgagttta aacagcacga
     2881 tgtgctccaa atattttagc cagtcaaaca gggcattcat gatatgtgta aatatttcat
     2941 tgttattggt tagcagaaca ttatcagtaa aggattttat caggtttttc tgcccatttc
     3001 acaaaaggca aaagtttttc gggaatattt atttcagtaa attctattct cctttcgctt
     3061 tggccaactt tcagttcttc atatatgaac gcatcatcaa aacctacttc agcagcatct
     3121 tttttagtag ctgcatatac tacccgctca gggcgtgccc aatagatagc tccgaaacac
     3181 atagggcaag gctcacatga agtgtacagt acacagtcat caagctgatg aaatttcaga
     3241 tgttcacatg catttctgat agctaccact tcggcatgtg cagtagggtc gaagttattt
     3301 gttacactat tgttaccttc gccaattatt ttaccatcct ttaccacaat agctgcaaac
     3361 ggtccgttgc catgttttaa tacattgtct gcagccagat taatcacata acgaataaat
     3421 tcttcatctt tttgggccat tgttctttgc ttttgaacaa atatacttat tattgtagta
     3481 ttattataac aactcaatat tgctttttac tagaagcaga aaaatacatc cggtacacat
     3541 ggaaaacaag atagacattg aagtatctaa aaatgaagac agcctcaagc ttgaagtaag
     3601 tcgtatcaag cgcttaatgc aggtgataga aaaaggaggt ggggaaaaaa ggctggagaa
     3661 aatacgaagc cagggcaaaa tgaccgcacg tgaacgcata gaatatctgc tcgataaaga
     3721 ccgtgaatac atagaaatag gcggctttgc tggatatggg atgtatgaag aaggaggagg
     3781 ctgccctgca ggaggtgtag tcatattcct tgggtacatc aaaggcaggc tttgcatcgt
     3841 ggtagccaat gatgcctctg tgaaagccgg tgcctggttc ccgattacag gaaaaaagaa
     3901 tctacgtgca caggagattg ccatggaaaa ccgaatacca atcatttatc tcgtggatag
     3961 tgcgggtgtt ttcttaccta tgcaggatga aatattcccc gacaaggaac atttcggacg
     4021 gatatttcgc aacaatgcca gaatgtcaag tatgggcata ccacagatag cagcgatcat
     4081 gggcagctgt gttgccggag gtgcctatct gcctatcatg tctgacgagg cactcattgt
     4141 cg
//
LOCUS       DAOQQY010000253         4089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10411_length_4089_cov_7.409519, whole genome shotgun sequence.
ACCESSION   DAOQQY010000253 DAOQQY010000000
VERSION     DAOQQY010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10411_length_4089_cov_7.409519"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLE23_10630"
     CDS             complement(<1..988)
                     /locus_tag="PLE23_10630"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMX83763.1"
                     /translation="MVRLEEPWYLLGVLIAISVIPALAYFRNKTIERISRFARPELMP
                     GYTAAEARKFRTKSYWLSIALVLLSVSLANPQWSTTKSTLSNSKADIFIVLDVSRSMT
                     AEDVAPSRLDKAKQFLYRLLSDMKSNNTGLILFAGRAYLQMPLTEDAAAVSSFINGAD
                     PDMIPAQGTAIGPSIDLAQRMFSKDSQKGKMILIISDGEDHDADAVEKAKAAKKHGII
                     VNTIGVGTAEGGTMPVYNVGIKDVKKDDEGNPIITRLNKAALKEIATTGGGKYFNISE
                     MDAAISYIASFASGKDTKNLRKQEFVDFESFFPWFLTPALLILLVLFFSHYLS"
     gene            complement(995..2020)
                     /locus_tag="PLE23_10635"
     CDS             complement(995..2020)
                     /locus_tag="PLE23_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMX83764.1"
                     /translation="MMDAGNILTQCLSGSMHWANAWVLWLIPVIWILYLAIKKRQVKS
                     FRFSDLSGIKTTATTKVSLLKFLPLLQLTGFSFLLLALARPQIFLKDETIKANGIDII
                     LSLDVSSSMLSKDFEPDRLSNAKKVADNFIDQRKYDRIGLVIFSGEAFTQCPLTSDHE
                     ILRELLKNVEPGILEDGTAIGMGLATAVNRLKNSQAKSKIVILMTDGVNNAGYIKPQT
                     ALNLAKTLGIKVYTIGIGSTGQALTPVARRYDGTYVYGVANVEIDEKLLSEIAESTGG
                     RYFRATTGTELSDIYRDIDRMEKTTIDVKTMKKYSEEYRYFLLIACMIFGLAAFLKYF
                     YIKPLIN"
     gene            complement(2017..2928)
                     /locus_tag="PLE23_10640"
     CDS             complement(2017..2928)
                     /locus_tag="PLE23_10640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83765.1"
                     /translation="MRYRVYQLVVVLICFISDNLYGQMPVITAVDSASYCVGDPILIR
                     LRIDDDRKEIKSVKTDTSVKANEAIEILDYGKWNKDSLDDIWRTSLTAAVYDSGRFTL
                     PEFRLLIEKDSSTDTLYAIPAEVSVSTLPVTDTTAIAPIKNIVRTPSSWRDYLPWMIG
                     AAAFLVVLLLALYFIRKRGKLPPEKPLLMTPVAAYELALTQLNRLKEIKPWEKGKIME
                     YQVELTQIIRQYMTNRYDIRALELSSDETLRAAKRKIPSAETLSYLSNLLNVADYVKF
                     AKAVPPDEIHEHVMEMALSFIKKTRQP"
     gene            complement(2925..3794)
                     /locus_tag="PLE23_10645"
     CDS             complement(2925..3794)
                     /locus_tag="PLE23_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMX83766.1"
                     /translation="MDATELYKQIRRIELKTKSLSHEWFAGQYHSAFKGRGMAFSEVR
                     EYQYGDDVRNIDWNVTARMDRPHVKVYEEEREINLTLIVDVSGSSAFTTGFHSKRDYI
                     ASLTATLAFSAIENNDTVGVIFFSDKIESVIPYGKSHKQVMRIIKEVLSIVPESAGSD
                     IAKALEYYLQYRKQRSVCVLISDFRSGNYEKQLKVAASKHDLIGIQVFDPGEKDIPDI
                     GLVPATDPETGRFLWLDTSDPHVRNQYRTSFDIQTRRFKEIFKKYGSDYLQMDTSESY
                     ARVLTGFFQSRQV"
     gene            complement(3796..>4089)
                     /locus_tag="PLE23_10650"
     CDS             complement(3796..>4089)
                     /locus_tag="PLE23_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83767.1"
                     /translation="KHDKTIAFNYDVRKKKEIMQDDIWNPKLNMQDTLAHYFESVGKP
                     GFSYKYFNLTPQGIRFYDDLNPLYGRNDVTIPYAVVRRWVKGKSPVRHLINPE"
BASE COUNT         1026 a          930 c          876 g         1257 t
ORIGIN      
        1 ctgacaggta atggctaaaa aaaagtacca gcagaatcag caaagcaggc gtcagaaacc
       61 aggggaaaaa gctttcaaaa tcaacaaatt cttgcttcct gagatttttg gtatccttgc
      121 ctgatgcaaa agaggcaata tagctgatag cagcatccat ttcgcttata ttaaaatatt
      181 ttcctccacc ggttgtagct atttctttta gtgcagcctt gttgagcctg gtgatgattg
      241 ggtttccttc atcatctttt ttcacatcct ttatgccaac attatagacc ggcatagtgc
      301 caccttctgc tgtgcctaca ccaatggtat tgacgatgat tccgtgtttt ttggcggctt
      361 tggctttttc aacagcatct gcatcatgat cttcaccatc gctaatgatc agtatcattt
      421 tgcctttctg cgaatccttg ctgaacattc gctgagcaag atcgattgac ggaccgatgg
      481 cagtaccttg tgccggaatc atatcggggt cggcgccatt gatgaaggag ctcacagcgg
      541 cggcgtcttc agttaaaggc atttgcaggt atgcccgacc ggcaaatagt ataagccctg
      601 tattattgga cttcatgtca ctgagtagcc ggtatagaaa ctgtttggcc ttgtcaagcc
      661 tggaaggtgc tacatcttct gctgtcatac ttctggatac atccaataca ataaatatgt
      721 ctgccttcga attgctcaag gttgatttgg tggtactcca ttgcggattg gcaagcgaaa
      781 cggataagag aacaagagct atcgaaagcc agtaagactt tgttctgaat ttccttgcct
      841 ctgctgcggt ataaccgggc atcagttcgg gtcgggcaaa tctgcttatt ctttcgatgg
      901 tcttattgcg gaaataggca agggcaggaa tgaccgaaat ggctatcaaa acgcccaaaa
      961 gataccaggg ttcttcaagt cttaccatgt ttctttagtt aataaggggt ttgatataaa
     1021 aatatttcag aaatgctgcc agcccgaata tcatgcaagc aataagcaag aaatatctgt
     1081 attcttcact gtattttttc atggttttta cgtcgatggt ggtcttttcc attctatcga
     1141 tgtctcggta aatgtctgat aattcggtac cggtggttgc cctgaagtaa cgtccgccgg
     1201 ttgattctgc tatttcgctg agcaattttt cgtcaatctc tacattggcg acaccataaa
     1261 cgtaggtgcc atcatagcgg cgcgctaccg gtgtgagtgc ctgacctgtg ctgcctatac
     1321 ctatcgtata tactttaatg ccgagtgtct ttgccagatt gagtgcagtc tgtggcttga
     1381 tatagccggc attgttgaca ccgtctgtca tcagtatgac gattttactt tttgcctggc
     1441 tgttcttaag ccggttgact gcagtagcaa gacccatacc tatggcagtg ccgtcttcaa
     1501 gtatgccggg ttctacgttt ttgagcaact cccgcagtat ttcgtggtca gatgtaagcg
     1561 ggcattgggt aaatgcttca ccagaaaaaa tgaccagacc gatacgatca tacttgcgct
     1621 gatcgatgaa gttatcggct actttttttg catttgacaa acggtcgggt tcaaaatcct
     1681 tcgataacat actgctcgag acatctagtg aaagaatgat gtcgatgcca ttggccttta
     1741 tggtttcatc cttgaggaat atctgtggcc gtgccagggc aagcagcaaa aaactaaaac
     1801 cggtaagctg cagcaaggga agaaatttta acaagcttac ctttgtggta gcagttgtct
     1861 ttattcctga aagatcagag aaacggaacg attttacctg cctcttttta atggccaaat
     1921 acaaaatcca tattacaggt atcagccaca gaacccaggc attggcccaa tgcattgagc
     1981 cggataagca ttgtgtcaag atattgcctg catccatcat ggttgccttg tttttttgat
     2041 gaaacttagc gccatctcca ttacgtgttc gtgtatctcg tcaggtggta cggctttggc
     2101 aaattttaca taatcggcta cattaagcag attgctcagg tagctgagtg tctcggctga
     2161 aggtatcttc ctctttgctg ctctcagggt ttcgtcactt gacagctcca aggcacgaat
     2221 gtcataacgg ttggtcatat actggcgaat aatctgggtt aactctacct gatattccat
     2281 tattttccct ttttcccatg gctttatttc cttcagtcgg ttgagctgtg tcagtgcaag
     2341 ctcataagct gccacaggcg tcatcagaag tggtttttca ggtggtagtt ttcctctttt
     2401 tctgatgaaa tacagtgcaa gtaatagcac aaccaaaaag gctgctgcac cgatcatcca
     2461 gggcaaataa tcacgccatg acgatggtgt cctgacaata tttttaatag gagcaatggc
     2521 tgtggtatcc gtaacaggca atgtactcac gcttacttcc gccggtatag catacaatgt
     2581 atctgttgat gaatcctttt ctatcaacaa gcggaactcc ggtagtgtaa aacggcctga
     2641 atcataaaca gccgcagtta aactcgtacg ccatatatca tccaggctat ctttattcca
     2701 ttttccataa tccagtattt ctattgcttc atttgctttt acggatgtat cagttttgac
     2761 cgattttatt tctttcctgt catcgtctat ccgcagcctg atgagaatag ggtcacctac
     2821 acaatacgaa gcactgtcca ctgcagtgat aacaggcatc tgtccataca gattatcact
     2881 gatgaagcaa ataagaacga cgaccaactg atagaccctg tatctcatac ttgtctgctt
     2941 tggaaaaacc cggttaatac tcttgcgtac gattctgatg tatccatttg cagataatct
     3001 gaaccgtatt ttttaaatat ttccttaaac cgtcttgttt gtatatcgaa cgaagtccta
     3061 tactgattcc gtacgtgtgg gtcactggta tcaagccata agaatctgcc agtctccgga
     3121 tcggtagctg gcaccagccc aatgtccggt atatcttttt cgcccgggtc gaaaacctgt
     3181 atgccgataa ggtcgtgctt cgaagcggct accttcaact gtttttcata attgccgctc
     3241 ctgaaatcac tgatcagcac acataccgat cgttgtttgc ggtattgaag gtagtattcc
     3301 agtgccttag ctatatccga gcctgccgat tcaggaacaa tgcttagcac ttcctttatt
     3361 atgcgcatca cctgcttgtg gcttttgccg tagggtataa cagattcgat tttgtccgaa
     3421 aagaaaataa caccaaccgt atcattgttc tcaatagctg aaaatgccag ggtggccgta
     3481 agtgatgcaa tataatctcg cttgctgtga aagccggtgg tgaatgctga cgagccactt
     3541 acatccacta taagggtgag gtttatttcg cgctcttctt catacacctt cacatgcggg
     3601 cggtccatac gggcggttac attccagtcg atgttgcgca catcgtctcc gtattggtac
     3661 tcgcgcacct cactaaatgc cataccacgc cccttgaatg cactatggta ttggccggca
     3721 aaccactcgt gacttaggct cttagtcttt aactctattc ttcgtatctg cttatacagt
     3781 tcagtagcgt ccatgtcatt ccggattaat cagatgccgt acagggctct ttcccttgac
     3841 ccatcttctt acaacagcat agggtattgt tacatcattg cggccgtaca gcggattcaa
     3901 gtcgtcgtag aatcttattc cctgtggtgt caggttaaag tacttatagc tgaaacccgg
     3961 tttgcccacc gactcaaaat aatgtgcaag tgtatcctgc atatttagct tagggttcca
     4021 aatgtcatct tgcattattt ctttcttttt ccttacatca tagttaaaag ctattgtttt
     4081 atcgtgttt
//
LOCUS       DAOQQY010000254         4061 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10515_length_4061_cov_5.254868, whole genome shotgun sequence.
ACCESSION   DAOQQY010000254 DAOQQY010000000
VERSION     DAOQQY010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10515_length_4061_cov_5.254868"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PLE23_10655"
     CDS             <1..910
                     /locus_tag="PLE23_10655"
                     /inference="COORDINATES: protein motif:HMM:NF023828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HMX83768.1"
                     /translation="LNCGAQVTHNFCSYCGQENTVHPESVGHIISHFFQDFTHFDSKF
                     FTSMKNLFFKPGHLTKEYNLGRRKRFINPIRMYIFVSLVVFSYLFFETKKTENIRLDL
                     DNTETSVLDQNKIKFSIGGKPVTPHSWKQDIDSTIIKYSPLAGDGYFTKKTKSLLVSS
                     LSRFKAEPDAFISNFLTNFMHNLPKTLLICLPFFALLLYLLYFNNRNYFVEHIIFSIH
                     FHIFLLLLFLLTMLLLKIDFLEVPVILIFPLVVLIYLYKSLGNVYKGSKLTTIMKEMV
                     LVMLYLVILYSVQILNVLFSLSGSHI"
     gene            916..2850
                     /locus_tag="PLE23_10660"
     CDS             916..2850
                     /locus_tag="PLE23_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMX83769.1"
                     /translation="MKKILTLLSFFCFSAPWLLAQDEICKSDFSQVKCSHAHIKNLHP
                     DLRTNLNTSNYNIIYNRLYFELDPSVRYIKGQVVAHYKFTNGTNDEIIFDLTDSLTVD
                     SIFYHQTKANFTHTNDQITIQLPASVPYDQLDSVRIYYQGVPPSNGFGSFEIGTHDGT
                     PVLWTLSEPFGSKDWWPYKLSLTDKLDSMDVFITTPDFFKAGCNGKLVNTYGKKGSMT
                     YHWQHRYPIAPYLLGIAVTNYEVFSNYVPMPDGSELEVLNYVYPESLLEAKANIPKII
                     PIIQLINQKVIPYPFAKEKYGHMQFGWGGGMEHQTMTSLKNFSFTLLAHEVAHQWFGD
                     HVTCGSWEDIWLNEGFATYFEGLCQEAINPWNWYTWKYNKIANITSNPGGSVLVDDTT
                     SVNRIFSSRLTYNKGSYLVHMLRWLLGDDDFYAALRNYLNDPKHAGGFAKTPDLIDAL
                     ETQSGKDLTKFFDQWYYNQGFPVYDILLNQDGQGNNKKLNIYQTQSHPSVDFFEMPVP
                     VQFFIGGKDTILRFDNTYNGQQFDIDLPGKVDSIKFDPELWLLAQYGSVSVSSGEIKE
                     AEGKFSLFPNPGIEGFFLTSPGEIVENIKILDPTGRLVKNEPSGLKGDFISASGLSSG
                     QYFIIIQTKSGIYSQKWIKN"
     gene            2835..>4061
                     /locus_tag="PLE23_10665"
     CDS             2835..>4061
                     /locus_tag="PLE23_10665"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HMX83770.1"
                     /translation="MDKKLITKIVFIFGFGFLSAYTSAQHTNVVIGTKNSPNEPSICI
                     NPKNTKQLVAGSNIANLYTSEDGGVTWTESVLQSPHGVWGDPIITVDTAGNFYFLHLS
                     NPLNGNWIDRIVCQRRNAGGQTWSDGGYAGLNGTKAQDKHSVVVDKNTNYLHITWTEF
                     DNYGSFSPQDSSRILYSRSTDLGDTWSTPVKLNKVSGDCIDSGNTTEGAVPAIGPNGE
                     IYVCWTGPAGIVFDRSTDGGNTWLDNDIHVSDHPGGWDFEIPGISRSNGLPFMVCDLS
                     GGPHNGNIYINWADQRNGNNVTDVWLVVSEDGGNTWSAPKRVNNDGTGKQQFFSSMTI
                     DPVTGYLWFVFYDRRNHSDNQTDVCMAVSKDGGNTFKNFKISETPFLPNSGIFFGDYT
                     FVTAYNNIVRPIWTRLH"
BASE COUNT         1230 a          871 c          809 g         1151 t
ORIGIN      
        1 tcttaattgc ggtgcacagg ttactcacaa cttctgctca tactgcggac aggaaaacac
       61 tgtacatccg gagtcagtag gacatataat ttcacatttt tttcaggatt tcacgcattt
      121 cgattcgaaa ttctttacaa gtatgaagaa tctgttcttc aaacccggac accttacgaa
      181 ggaatacaac cttggaagaa gaaaaagatt catcaaccca atacgcatgt acatctttgt
      241 gagtctcgtg gtattctcct atctgttttt tgaaactaaa aaaacagaga acatccgact
      301 cgatcttgac aataccgaaa catctgtatt agaccagaat aaaatcaaat tttcaattgg
      361 aggcaaacct gtaacaccgc acagttggaa acaagatatt gactcgacca tcatcaaata
      421 ttctccctta gccggtgatg gatattttac aaaaaaaacc aaaagtcttc tggtaagttc
      481 cttgtcaaga ttcaaagcag aacctgatgc ttttattagc aattttttga ctaatttcat
      541 gcacaatttg cccaaaacgt tactgatttg tcttcctttc ttcgcattac tgctgtatct
      601 gctgtatttc aataatagga attatttcgt tgaacacatc attttcagta ttcatttcca
      661 tatattttta ctcttgttgt tcctactaac gatgttgctt ttaaagattg attttcttga
      721 agtccccgtc attcttattt ttccgttggt agtattgatt tatctgtaca aatcgctggg
      781 aaatgtatat aaaggtagca aattgacaac aatcatgaaa gaaatggtgt tggtaatgtt
      841 gtatttagta attttatatt cggttcagat attaaatgta cttttttcac tttccggtag
      901 ccatatttaa taaaaatgaa aaaaatttta actctactct cctttttctg cttcagcgct
      961 ccctggctgt tagcccaaga cgaaatatgt aaaagcgatt tttcgcaggt taaatgttca
     1021 catgctcata tcaaaaacct tcatcctgat ttgcgcacaa accttaacac aagcaactac
     1081 aatattatat acaaccgatt gtacttcgag cttgacccct ctgtaagata tatcaaaggg
     1141 caagtagtag cacattacaa attcacaaat ggaacgaatg acgaaatcat attcgacctg
     1201 actgattcgc ttactgttga ttccatattt tatcatcaga caaaggccaa ttttacccac
     1261 accaacgacc aaatcaccat tcagctacct gcttcggtac catacgacca actcgattct
     1321 gtgcgtatct attaccaagg tgtaccgcca tccaatggtt ttggttcatt cgaaataggc
     1381 acacacgatg gaactcctgt cctctggaca ctatctgaac catttgggtc gaaagactgg
     1441 tggccctaca aactctcact taccgacaaa ctcgattcta tggatgtatt catcactaca
     1501 cccgattttt tcaaggcagg ttgtaacggc aaacttgtaa atacttatgg aaagaaaggt
     1561 tcaatgacat accactggca acaccgatac ccgatagcac cctatctttt gggtattgca
     1621 gttacgaatt atgaggtctt ttctaactac gtccctatgc cggatggttc agaactcgag
     1681 gtgctcaact acgtgtatcc cgaatcactg ttagaggcaa aagccaatat tccaaagata
     1741 atcccaatca ttcaactgat caatcaaaag gtcatcccgt acccgtttgc caaggaaaaa
     1801 tatggtcaca tgcagtttgg ctggggtggc ggtatggagc atcagacgat gacctcatta
     1861 aaaaattttt catttaccct gttggcacac gaagtagcac accagtggtt tggtgaccat
     1921 gtcacatgcg gcagttggga agacatctgg ctcaatgaag gctttgctac ctactttgaa
     1981 ggcttgtgtc aggaagctat caacccatgg aattggtaca cctggaaata caacaagata
     2041 gccaatataa caagcaaccc gggtggctcg gttttggtag atgacactac gagtgtaaat
     2101 cggatattca gttcaagact tacttacaac aaaggttcct atttggtcca catgctgcgc
     2161 tggttattag gtgatgatga tttctatgct gcattgcgca attaccttaa cgatccaaaa
     2221 cacgccggag gatttgcaaa aacacccgat ttgattgatg cacttgaaac tcaaagtggg
     2281 aaagatctta caaaattttt cgatcaatgg tactacaacc agggtttccc ggtgtatgac
     2341 atattactga accaggatgg gcaaggaaat aataagaaac tgaatattta ccagacccaa
     2401 tcacatccgt ccgtagattt ttttgaaatg cctgtaccag ttcaattttt tattggcggt
     2461 aaagatacca ttctccggtt tgacaatact tacaatggtc agcagttcga tatagaccta
     2521 cccggcaagg tggattctat caaatttgac cccgaactat ggttgcttgc tcagtatggt
     2581 tcagtatcgg tttcttcagg tgaaataaag gaagctgaag gaaaattttc actattccct
     2641 aaccctggta ttgaaggatt ctttcttaca tccccgggcg agatagtcga aaatatcaag
     2701 atattggatc ctacgggccg cttagtaaag aatgagccat caggcctcaa aggagatttc
     2761 ataagcgcat caggcttgag ctcaggacaa tattttatca taatacaaac aaaatcaggc
     2821 atatattcac agaaatggat aaaaaattaa tcacaaaaat tgttttcata tttggttttg
     2881 gctttttgtc agcatacaca tcagcacaac atacaaacgt ggtcattggc accaaaaaca
     2941 gcccgaatga accgtctatc tgcatcaacc cgaaaaatac caagcaactt gtagccggta
     3001 gcaacattgc caatctatat acttcagaag atggcggtgt tacatggaca gagtctgtat
     3061 tgcaaagtcc acatggtgtg tggggcgatc cgatcataac ggttgataca gcaggcaatt
     3121 tttacttcct acacttatcg aacccactaa atggcaattg gattgacagg attgtatgtc
     3181 agcggcgcaa tgcaggtgga cagacatggt cggatggtgg ttatgccgga ctcaacggta
     3241 ccaaggcaca ggacaagcat tctgtcgttg ttgacaaaaa caccaattac ctacacatca
     3301 cctggaccga atttgacaat tacggaagct ttagtccgca agatagttcg agaatacttt
     3361 acagccggtc aacagatttg ggtgacacct ggagtactcc ggtgaagctc aacaaagtgt
     3421 cgggcgattg tatagacagt ggtaatacaa cagaaggggc tgtaccggct ataggaccca
     3481 acggtgaaat atacgtctgc tggaccggac cggccggcat tgtatttgac agatcaacag
     3541 atggtggcaa tacctggcta gacaacgaca tccatgtgtc ggatcatccg ggtggatggg
     3601 attttgagat tccgggcatt tcgaggagca atgggctgcc cttcatggtt tgtgaccttt
     3661 caggcgggcc acacaatggt aatatttata tcaactgggc agaccagcgc aatggtaata
     3721 atgtcacaga tgtctggttg gtagtctcag aagacggtgg caatacctgg tcggcaccca
     3781 aacgtgtcaa caatgatggc acaggcaagc aacagttctt tagttctatg accatcgacc
     3841 cggtgacggg ttatctttgg tttgtatttt acgatcgcag aaatcattcc gacaaccaaa
     3901 ccgatgtgtg tatggcagtt tcaaaagatg gaggcaatac attcaaaaat tttaaaatca
     3961 gcgaaacgcc ctttttgccc aattccggaa tcttctttgg tgactacaca ttcgttacag
     4021 catataacaa tatcgtaaga ccgatttgga cacggctgca c
//
LOCUS       DAOQQY010000255         4059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10525_length_4059_cov_5.787213, whole genome shotgun sequence.
ACCESSION   DAOQQY010000255 DAOQQY010000000
VERSION     DAOQQY010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10525_length_4059_cov_5.787213"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="PLE23_10670"
     CDS             <1..1233
                     /locus_tag="PLE23_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83771.1"
                     /translation="EIMGVLACLTWLVMPIFAYHYFNEGNYLAKLWLIYMYVPSDTYY
                     EVVFWGTVAMLIGLHFPFSKLKILAAKEYTETIKRNSQNYGKISKILLVIGVLSLLLG
                     PVVPAVLGQVFEITGNFIFVAAFYAYYSQDRNKFWLVSVVVAIIIGISIYGGMFGKLV
                     FISILAAMIVLGVRERKLSMFRKLTISIVGIILIIILQSVKHEYRKNTWSGYAGYTGY
                     SGSKTALFFDLIGEKLKDPFAIFQDEAQLFALTARFNQGWLISKAINYIPAKQPFVGP
                     GPLTAAIAGGFIPRFLWPDKPESGGKANMLRYAGVQLKQVSMNVSPVGEAYGSFGMPG
                     AWIFMFFYGLFFRWSFLKILEIAKTHPTLLLWLPSIYVLTVVVEGDVLTVFNAFTKSV
                     LMVWLFYVGFRKILKINL"
     gene            1289..2482
                     /locus_tag="PLE23_10675"
     CDS             1289..2482
                     /locus_tag="PLE23_10675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMX83772.1"
                     /translation="MYSGTNTKKPSLGKYIKAELTDSDIPLSASLKLRIIGLKLYIEN
                     SILRFFTRILFRPATHPKKILVFRTGSLGDSINAIPALHAIKDTFPDAQIDILTNAGL
                     KNLVGMRYLLDKNLYHEIIDYYGMPKRELFRFLKEKQYDCVIQLPQANAVFHRVLRDL
                     LVFRGISKSGIGWYTSHTGLFRKTQAKYLIFNNEHEKLLAHLRQAGFKTNQLTPILNP
                     SNADKEFVQHLLHENGLNDGKKKLCVVIGAKRPQNRWPIGYFKVVIEHFAPSVHILLI
                     GSQEDRLLAEPLLGIQHVHNFCGLLTPMQSAAMFGMSDLTLSNDTGPMHMSYAVSTPT
                     IAIFSARDLPGKWYPPVENNLVLRAKGIACEACMSEVCHNNICMQAILPEQVIEAVEH
                     FLDKV"
     gene            2639..>4059
                     /gene="asnB"
                     /locus_tag="PLE23_10680"
     CDS             2639..>4059
                     /gene="asnB"
                     /locus_tag="PLE23_10680"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMX83773.1"
                     /translation="MCGITGILSTSLPLEATARSMADAMGHRGPDASGYYIDEYVSLG
                     HRRLAIIDLSPQGNQPMYSADGRYVIVYNGELYNYKELREKLRALYPFKTNSDTEVIL
                     AAFRQWGPHCLQDFNGIFAFAIWDTRTRKLFVARDQLGVKPFYYYRTDTGFLFASELR
                     GLLASKVLRPTLSLQGLTDFLSYQSVHEPSSMISGTYQLKAGHYAWVTDKSFVQSRYY
                     KGSEVVQHHDCDHQRHKQNIKDLFMQAVNRQLVSDVPLAVFLSGGIDSSAIVAAAAQS
                     SSGTINTFSIAFEEAKYNESKYARLVADKYKTNHVECKVPATIFIDQIEDALNAMSTP
                     SGDGPNTYLISGLVKSAGIKVALSGLGGDELFAGYPGFMYYYRLQALQSKLPGWLRQG
                     FGSVFTNFPNRKLQKMAELFSKPDFDIAYVYPAIRRIYHKSELLRLNELLGHCHDTIE
                     AGLLSDSKNINQLPILSRYSLAEL"
BASE COUNT         1110 a          905 c          885 g         1159 t
ORIGIN      
        1 gagattatgg gagtgcttgc atgccttacc tggctggtga tgcccatatt tgcttaccat
       61 tattttaatg aaggaaatta tcttgccaag ctatggctga tatatatgta tgtgccgagt
      121 gatacctact acgaggtagt attctggggt acagtagcca tgttgatagg cctgcatttt
      181 ccgttcagca aactcaaaat tctcgccgcc aaagaatata cagaaacaat taagcgtaac
      241 agtcagaact atggcaaaat cagtaagatt ttactcgtta tcggtgtatt gagtttgcta
      301 cttggacccg tagtgccggc tgtactcggg caagtatttg agataacagg taactttatt
      361 ttcgtagctg ccttttatgc ctattattcc caggatcgga ataaattctg gttagtttct
      421 gtggttgtag ctattattat aggcatatct atatatggcg gtatgtttgg caaattggta
      481 ttcatctcta tactcgctgc catgattgta ctgggggttc gtgaacgcaa actaagtatg
      541 ttccgaaaac tcaccatcag catagttggt attatactta ttatcatttt gcaaagtgtc
      601 aaacacgaat acagaaaaaa tacctggagt ggctatgccg gatatacagg ctactcaggg
      661 agtaagacag cattattctt tgatttaatc ggcgaaaaat tgaaggatcc ttttgctata
      721 tttcaggatg aagcacaatt attcgcactg actgccagat ttaatcaggg ctggctgata
      781 tcaaaagcca tcaactatat accggccaaa cagccctttg taggcccggg tccgttgaca
      841 gcagctattg caggtggctt tattccgcgt ttcctctggc cagataagcc ggagtcaggc
      901 ggtaaggcca atatgttgcg ctatgcaggt gtgcaattaa agcaagtaag tatgaatgtc
      961 agtccggtag gtgaggctta cggcagcttt ggcatgcccg gggcctggat atttatgttc
     1021 ttttatgggc ttttttttcg ttggtctttt cttaaaatac tggagattgc taagactcat
     1081 ccaaccttgc tcctctggct gccttccata tatgtgttga cagtggtggt agagggtgac
     1141 gtgttgacag tcttcaatgc cttcaccaag tcggtgctga tggtgtggtt gttctatgtc
     1201 ggttttagaa aaatactgaa gatcaatctt tagttgatgt caacaagctt tgctttgtac
     1261 taatcttatt attgtcaaat aaaaaataat gtactcgggt actaatacga aaaagccatc
     1321 acttggcaag tatataaagg ctgagctgac cgattcggat attccgctgt cggcaagctt
     1381 gaagctccgt atcatcggtc tcaaactcta tatcgagaac agtattctcc ggttttttac
     1441 cagaatcttg ttccgtccgg ccacccatcc taaaaaaatc ctggttttcc gcaccggctc
     1501 tttgggcgat tctatcaatg ctattccggc tttacatgcc ataaaagata cttttcctga
     1561 tgctcaaatt gacatcctta ccaatgccgg actaaagaat ctggtaggca tgcgttacct
     1621 gttggacaaa aacctgtacc acgagatcat tgattattat ggaatgccca agcgcgaact
     1681 tttcagattt ctaaaagaaa aacaatatga ttgcgttatt caattgccac aagccaatgc
     1741 cgtttttcac agagtgctca gagatctttt ggttttccgc ggcatcagca agtcaggtat
     1801 cggctggtac acgagtcata caggcttgtt caggaaaacc caggccaaat acctgatttt
     1861 taacaacgaa cacgaaaaac tccttgctca tctcaggcag gcaggtttca aaaccaacca
     1921 attgacacca attttaaatc catcgaatgc agacaaagag tttgtgcagc acttacttca
     1981 cgaaaatggc ctgaatgacg gcaaaaagaa attgtgcgtg gtgataggag ccaagaggcc
     2041 tcaaaacagg tggcccatcg ggtattttaa agtagtcatt gagcatttcg cgcccagtgt
     2101 ccatatctta ctcatcggct cacaagaaga ccgcttattg gctgaaccat tgctcggtat
     2161 tcagcatgtc cacaactttt gcggactact tactcccatg caaagtgccg ccatgtttgg
     2221 tatgagtgac ctgacactta gcaacgatac aggccccatg cacatgtctt atgctgtcag
     2281 tacacctact atagctattt ttagtgccag agaccttccc ggcaaatggt atccgccagt
     2341 cgaaaataac ctggtattgc gtgctaaagg aatagcatgt gaggcgtgta tgagcgaggt
     2401 gtgtcataac aacatctgta tgcaggctat cttgcccgag caagtaattg aggcggttga
     2461 gcattttcta gacaaagtat aaaagagctg tttgccttaa tggtactgaa ttaaatattt
     2521 tactattttt attctgtttc ataatatttt tctttcacac cgcattgtac tataaaaatt
     2581 ttaccagtac atttgcacgc ttttttacaa acattaacat aacactttta aacaaaatat
     2641 gtgtggcata acaggtatct tgtccacctc cttaccgctt gaagccacag cgcgctccat
     2701 ggcagatgct atgggccacc gaggcccgga tgcttcaggc tactatatcg atgagtacgt
     2761 ctcacttggc catagaaggc ttgctatcat cgaccttagc ccgcagggca atcaacccat
     2821 gtacagcgct gacggtcgat atgtcatcgt ctataatggc gaactctata attacaaaga
     2881 actcagagaa aaactcagag cactatatcc attcaaaacc aactccgata cggaagtaat
     2941 actggcagca ttcaggcagt ggggccccca ttgcctccaa gactttaacg gcatttttgc
     3001 cttcgccatc tgggacacca gaacccgaaa gctattcgta gctcgcgatc agcttggtgt
     3061 caagccgttt tattattatc gtacagatac cggcttttta ttcgcttctg agttgcgcgg
     3121 tctgctggcg tctaaggtgc tgcgtcctac attgagtttg caagggctta ccgatttcct
     3181 ctcataccag tcagttcacg agccgagctc catgatctca ggtacttacc agctcaaggc
     3241 cggtcattat gcctgggtta cagacaagag tttcgtacag tcgcggtatt ataaaggtag
     3301 tgaagtagta cagcatcatg actgcgacca ccaaagacat aaacaaaaca tcaaagacct
     3361 gttcatgcag gcagtcaaca ggcagttggt gagcgatgta ccactcgctg tgtttttatc
     3421 gggcggcatc gactccagtg ctattgtagc tgctgctgca caatcttcat ccggtacaat
     3481 caataccttc tccatagcat ttgaggaggc aaaatacaat gagtcgaagt acgccaggct
     3541 ggtggccgac aagtataaga ccaatcatgt agagtgcaag gtaccggcaa ctatatttat
     3601 tgaccagata gaagatgcac tcaatgccat gagcactcca agtggcgacg gccccaatac
     3661 ttatctgata tcgggtctcg tgaagtctgc cggtatcaaa gtagcactct caggcttggg
     3721 aggtgatgag ctttttgccg gttatccggg ttttatgtat tactaccgct tgcaagcact
     3781 gcaaagcaag ctgcccggtt ggttaaggca aggttttggc tccgttttta ccaatttccc
     3841 taaccgaaaa ttgcaaaaaa tggcagagct cttctccaaa cccgattttg acatagctta
     3901 tgtttatccg gccatcagac gcatctatca caaatcagaa ttgttaagat taaatgaact
     3961 tttgggccat tgccatgaca caatcgaagc aggattgcta tcagacagca agaacatcaa
     4021 tcagctgccc atattgagca ggtattctct ggccgaact
//
LOCUS       DAOQQY010000256         4046 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10586_length_4046_cov_5.465046, whole genome shotgun sequence.
ACCESSION   DAOQQY010000256 DAOQQY010000000
VERSION     DAOQQY010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10586_length_4046_cov_5.465046"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLE23_10685"
     CDS             complement(<1..239)
                     /locus_tag="PLE23_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMX83774.1"
                     /translation="MIPFLDLHRQYLTIKQDIKAAIEDVIEQTAFSGGPFVERFENHF
                     ARFCDTSYAVGLNSGTSALHLAMEALGIGKGDEVI"
     gene            complement(246..737)
                     /locus_tag="PLE23_10690"
     CDS             complement(246..737)
                     /locus_tag="PLE23_10690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMX83775.1"
                     /translation="MEILNIDSPKQSLRNVKVGKDVKIYDFVNAYGCEIGDGSKVGTF
                     VEIQRGAYIGKNCKISSHTFICEGVHIGDGVFVGHNVSFINDKYPQAVNPDGSMQTDA
                     DWNMVETKVGNHASIGTSATILCGVEIGDAAVIGAGSVVTKNVPPKAVVAGNPAKIIK
                     YID"
     gene            complement(739..1743)
                     /locus_tag="PLE23_10695"
     CDS             complement(739..1743)
                     /locus_tag="PLE23_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMX83776.1"
                     /translation="MKTAIVGYGYWGPNLVRNFNSTEHSEVVMVFDLSEKQLERVRKA
                     YPKIHTTTRYEDIIDNPEIDAVAIATPVFTHFGLARQALLAGKHVLIEKPMTSSVAEA
                     RELMEIAGQNNKVLMVDHTYLYTDAVQKIKQLVQAGEIGKVQYIDSTRINLGLFQRDV
                     NVLWDLAPHDISICNYLMDDFPVAVQAVGAAHTANNLANIAYLTLFYQDNKIAHFNCS
                     WVSPVKIRQMLIGGDKKMILFNDLETTEKLRIYDKGYSIMPESDRDRLLIDYRIGDIT
                     IPKIPQTEALTSMAKDFIRSIQTGNAPLSSSTSGLQVVKILEAAEKSIQNKGKYIEFE
                     "
     gene            complement(1740..2456)
                     /locus_tag="PLE23_10700"
     CDS             complement(1740..2456)
                     /locus_tag="PLE23_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX83777.1"
                     /translation="MQSWSIGILCYNEAGTIKDVYLKAKSVAARLSDQYEIIVVDDCS
                     TDGSRDIIHTICDTDHAVRGIFHEKNLGIGLSIRDIYFNAKNENVVFIPGDGQFDSEE
                     LLPYASFSDKNYIAFYRKENQSYSFFRNSLSYMNKLVNSLFIGLKLRDVNWVKVYKTH
                     ILTQLDLKAQSSFIESEICAKLNRLFIKPIEVDSKYIPRVYGTSKGASWAIIRKVYIE
                     LFKIFINLRQFDPTKVNIDK"
     gene            2582..3796
                     /locus_tag="PLE23_10705"
     CDS             2582..3796
                     /locus_tag="PLE23_10705"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMX83778.1"
                     /translation="MARDFEKLLSGFVNRIEESSTLRMAQMARNLKAKGYDVINLSLG
                     EPDFDTPQHIKDAATAALNAGITKYTPVAGLLELNQAIVEKFKRDNNIEWATENIVVS
                     NGSKQSFANVCFALINPGDEVVIFSPYWVSHYEIVKMTGGVPVEVKAGIDQNYKITAQ
                     QLEKALSPRTKMLVFSSPCNPTGSVFSKEELEAFAEVLQKYPDVIIVSDEIYEYINFT
                     GNHTSIGSLPSVADRTVTLNGFAKGFAMTGWRIGYMGAPKEIAKACVKFQGQVTSGAS
                     SFGQYASSVALRSDLKPTFDMVKAFKKRRDLLISLVKDIKGMKCNKPEGAFYLFPDIS
                     AFFGKSYGKTKIKTAEELCEYLLNHAHVALVGGEGFGAPNCLRISYAASDQDIRKAMN
                     RIKEALEELKYT"
BASE COUNT         1067 a          914 c          880 g         1185 t
ORIGIN      
        1 aaaatgactt catctccctt gcctatacca agtgcttcca tggcaagatg caaggcagat
       61 gtgccactgt tgagcccgac agcatagctt gtgtcgcaaa agcgtgcgaa atggttctcg
      121 aatctttcaa caaacggccc tccggagaac gcagtctgtt cgataacgtc ttcgatggca
      181 gcttttatat cttgtttgat ggtcaggtac tgccggtgca ggtctaagaa tggtatcatg
      241 tgtgattagt ctatgtattt aatgattttg gcaggattgc cggctaccac tgctttgggt
      301 ggtacatttt tggtaacgac actaccggcg cctatgacag cggcgtcacc tatctctact
      361 ccgcacagta tggtggcact tgtaccgatg gaggcatggt tgcctacctt ggtttctacc
      421 atattccagt cggcatctgt ctgcatcgaa ccatccgggt tgaccgcctg tgggtatttg
      481 tcattgatga agctcacatt gtgacctaca aaaacaccat cgccgatgtg tacgccttcg
      541 cagatgaagg tatggctcga tattttgcag tttttgccga tgtatgcgcc tcgttgtatc
      601 tctacgaatg ttccgacctt gctgccgtca cctatttcac agccgtaagc atttacaaaa
      661 tcgtagatct ttacatcctt gcctaccttt acgtttcgga gcgattgttt gggtgagtca
      721 atattcagta tttccatatt attcgaattc tatatatttt cctttgtttt ggattgattt
      781 ttcggctgct tcgaggattt ttactacttg caggccgctg gtagaagatg acagaggagc
      841 attgccggtc tggatagagc ggataaagtc ttttgccatg gatgtcaatg cttcagtttg
      901 cggtattttg ggtatggtaa tgtcaccgat gcggtaatct atgagtaagc ggtcgcggtc
      961 actttcgggc ataatgctgt agcctttatc gtatatgcgg agcttttcgg ttgtttcgag
     1021 gtcattgaac agaatcattt ttttgtcgcc acctatgagc atttgcctta tcttcacagg
     1081 tgatacccat gagcagttga agtgggcaat cttgttgtct tgataaaaca aggtcagata
     1141 tgcaatattg gccagattgt tggccgtatg tgcagcacct actgcctgta cagctacagg
     1201 gaaatcatcc atcaggtaat tgcagataga gatgtcgtgt ggtgcgaggt cccagagtac
     1261 attcacatcg cgttgaaaca agccgagatt gatgcgcgta ctgtctatgt actgcacttt
     1321 tcctatttcg cctgcctgta ccagttgctt tattttctga acagcatctg tataaagata
     1381 agtatgatct accatcagga ctttattgtt ctgtccggct atttccatca gttctctggc
     1441 ttcggctacc gaactggtca taggtttttc gatgagcacg tgtttgccgg ccagtaatgc
     1501 ctgacgtgca agcccgaagt gggtgaaaac cggcgttgcg atggcaacag catctatctc
     1561 aggattgtca atgatatctt cgtaacgagt ggtcgtgtgt attttcggat aggctttgcg
     1621 cacacgttcg agttgttttt cgctcaggtc gaataccatt actacttctg agtgttcagt
     1681 agagttgaaa tttctcacca ggtttgggcc ccagtatccg tatcctacga tggctgtttt
     1741 catttatcaa tatttacttt agttggatcg aattggcgca ggttaatgaa aattttaaaa
     1801 agctcaatat atacctttct gatgatagcc catgaagcgc ccttactggt accataaacc
     1861 cgcggaatat atttagagtc aacttcaatt ggctttatga acagccggtt gagcttggca
     1921 catatttcac tttctataaa agaactttga gccttcagat cgagttgcgt cagaatatgt
     1981 gttttataga cctttaccca gttgacgtct ctgagcttta gccctatgaa taatgaattc
     2041 acaagcttgt tcatgtatga aagcgaattt ctgaaaaacg aatacgactg attttctttt
     2101 cggtaaaacg ctatataatt cttatcggaa aatgaagcat agggtagcaa ttcttctgaa
     2161 tcaaactggc cgtcgcccgg tatgaataca acattttcat tcttcgcatt gaaataaata
     2221 tcacgtatag acaaaccaat tcccaggttc ttttcgtgaa atattcctct tactgcatgg
     2281 tctgtatcgc atattgtgtg gatgatatcg cgcgagccgt ccgtactaca gtcgtccaca
     2341 actatgattt cgtattggtc gctcaatctg gcagctaccg attttgcttt gaggtacaca
     2401 tctttgattg tacctgcttc gttgtaacac aaaataccaa tagaccatga ctgcataaac
     2461 cggaattgac cgtaaaaata tataataacg cacgaaacga ggatttgatt tggaataaat
     2521 tgcttaagtt ttcttttttc tggcttttag catcatcttt gcataaaatt ttaatatact
     2581 tatggcacgc gattttgaaa aactcctgtc aggatttgta aatagaattg aagagtcgtc
     2641 aactctcaga atggctcaaa tggctagaaa tctcaaggca aaaggctatg atgtcatcaa
     2701 cctgagcctt ggtgagccgg atttcgatac accacaacac atcaaagatg ccgctacagc
     2761 tgccctcaat gccggtatca ccaaatatac accggtagcc ggactgctcg aactcaatca
     2821 ggcaatcgta gaaaaattca agcgcgataa taatatagag tgggctaccg aaaacatcgt
     2881 ggtatctaac ggctcgaagc agagtttcgc caatgtctgt tttgccttaa tcaaccccgg
     2941 tgacgaagtg gtcatctttt cgccttactg ggtatcgcat tacgaaatcg tcaaaatgac
     3001 aggtggtgtc ccggttgaag taaaggccgg tatcgatcaa aattacaaaa taacagccca
     3061 acaactcgaa aaagctcttt cacccagaac caaaatgctc gtattctcga gtccatgcaa
     3121 cccgaccgga tctgttttct caaaagaaga actcgaagcg tttgctgaag tattgcaaaa
     3181 ataccctgac gtcatcatcg tgtcggacga gatatacgaa tacatcaact ttaccggcaa
     3241 tcatacaagc atcggttcgc ttccttctgt agcagatcgt accgtcacgc tcaacggctt
     3301 tgccaaaggc tttgccatga ccggctggcg aataggctac atgggcgcac ctaaggaaat
     3361 agccaaggca tgtgtcaaat tccaggggca ggtcacctca ggtgcatcct ccttcgggca
     3421 gtatgcctca tcagtagcat tgcgctcaga tctaaaacca acattcgata tggtcaaagc
     3481 cttcaaaaaa cgccgagacc tcctcatcag cctggtcaaa gacattaaag ggatgaaatg
     3541 caacaaaccc gaaggcgcct tctacctctt ccccgatatc agtgcctttt tcggaaaatc
     3601 atacggcaaa acaaagatta aaaccgccga agaactctgc gaatacctgc tcaatcacgc
     3661 tcacgtcgct ctcgtaggtg gcgaaggttt cggcgctccc aattgcctgc gcatcagtta
     3721 tgccgcctcc gaccaagaca tacgcaaggc aatgaaccgc atcaaggaag cactcgaaga
     3781 actgaaatat acctaaggtt cgtcagtaga gcgcaagtac agaatgttat tcaacaggag
     3841 taaacattgc aagccaatat cgtagctaaa tctgatgaac cggcaaggat ttattcgtat
     3901 atggtatttt atatatacta acccgtcttg atttgttgat tagggcgtat ccctgtcggg
     3961 tcgggctatt cggcttactt cgtgccgcct gctatccctt acctttactt tgcatttgag
     4021 attaaattct taactttaac aaaggg
//
LOCUS       DAOQQY010000257         4044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10591_length_4044_cov_10.293307, whole genome shotgun
            sequence.
ACCESSION   DAOQQY010000257 DAOQQY010000000
VERSION     DAOQQY010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10591_length_4044_cov_10.293307"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            485..>4044
                     /locus_tag="PLE23_10710"
     CDS             485..>4044
                     /locus_tag="PLE23_10710"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX83779.1"
                     /translation="MMSGAFITKMLAKESPYNAIAEPTEDCVKAKFTIQGIRCISATK
                     FEVCVQASQAGTPNYWEFKLGNVTQTISQLGSVCISIEDPNLTGPPILSIKHIKDGPM
                     GTIFCTKSFNLLCGTNCDDLEFTTEYFFCHADFTLNYPASNAVLWDFGDGSLGITPAG
                     QNAVSHNFPTTGGDFTICQTQVIDTGQDTLDVNVCSKMISVPPCSENICDNMILEMNK
                     TCNGSEESYVEFHIKHYQNVATYRWNFGDGTPSHTSITPDAMHTYDKSGCYNICITLY
                     GGHGDLIATCCYRKCINCCSADDFDFYSVLPTGSCFNQKFVLENHCGKGSNLKHIWEF
                     EDGKVYYGENPPAEYVFSNFVNEDHEVCVRHTIICCNDTITATKCLPFTKGVFVGYAG
                     KTTKATDFASWWGHPAIRIYQMIDQYSNVPGLPLFMDGTYHHDLSHTYPSGTWNMGYS
                     AVIYVNSDAIIKYKPESNPNAPITFGLEGTVIQDAKRFHPQYGTCCRWEGIRNNGPNR
                     FEWDAAQITDAKKMLDLSTNAIYNNELAVLKIEDCRFYENIYGIYSKGFSFTVPSFSN
                     NVFDGPHHPHETCDCDVEDAIHLENISSPPVVFPNNKATDNEIMVYQNGLFASNSNVK
                     FCSFNIHDQATYALLDKDNIGNGIYYETNKGLNYSLTCDYIKVKNFKAGITQRSVGLG
                     TQSLMAIADVPYSSIWFENVTKGIDIDATNVMLNTRVVGNKIITNGGDNNLGVVVKTK
                     GVPAKKAHFVQIDNNDIYCNGGLGTKVGIGILNNNFNKQYFSISRNKVTHNYDPGVGI
                     FVSNGRNTKITNNYVDVKTSSSNGIEILDSDFSKISCNTFMGGELGMRWANSKYTLLS
                     QNYTGDNNVGWSIEGACDASKIEWNRFDYTKSYSINYYDNTTFTGKQAHRRYNTWTSQ
                     NGKEINTLEPLKNQMWCDGSQAVGTVHRPQADASGVFFDGTVYTYLLPQDSCPVLQNH
                     DNGDNLDGEGVLQNCIHRINDTLSLAGYTDVQRCNTEQSLLQSIRENPDWMLTSTVIS
                     QFAGQHINDFVGKSVTVQMMMSQVQAEVDTLQSYLRPYLVELDSLLEIRAQLIEDLNT
                     ASDSVTYAVISEQLSENDTILTQLQGYIDNLNAQNDIIISAMLGAIESLNASISVSGL
                     HEYCEKNVNQYYLKTWRGDSLSSGEKADLWDIANTCYDDGG"
BASE COUNT         1265 a          760 c          898 g         1121 t
ORIGIN      
        1 cggtatcccc gataatgcgg taattaccag tgtgtttctc aagtttaagt atgctggtgt
       61 tatcgggacc tcacctttta atacccatgg aaatttgtta gttgatgtac gtaaagggtc
      121 tttcactaat aatgtaaact tgcaattgaa cgattttaag tcaacggcta gtaaaaaaaa
      181 tatattgagt tacaatgcgt cgaaagtggg taattggtat acgcgttcat taggcccaac
      241 cttttatcgg tttattaata aaacgggtta tactcaattt cgcctgcact ttactctcga
      301 cgataacaat gattttggct tggatttact aaaaatatac agtggaaatg ccccaacagc
      361 tcgaaggccc aacagcccaa cagcccaaca gcccaacagc ccaacagccc aacagcccaa
      421 cagtaggcat tattgtggaa tttatttgtt gcgaaaatta ttaaattatt tgttaataat
      481 ttttatgatg tcaggagcat ttattacgaa aatgttggcg aaagagtcgc cctacaatgc
      541 gattgcagaa ccaacggaag attgtgtgaa ggcaaaattt acaatacaag ggataagatg
      601 tataagtgct accaaatttg aggtatgtgt acaagcttct caggcaggta cgcctaacta
      661 ttgggagttt aaacttggaa acgtaacgca gacaatttcg caattgggca gtgtctgcat
      721 aagcatagaa gaccctaatc tgaccggccc accgatacta agtataaagc atataaaaga
      781 cggaccaatg ggtactattt tttgtacaaa atctttcaat ttgctttgtg gtaccaactg
      841 tgatgacctt gaatttacta cagaatattt cttttgccat gccgatttca cattaaatta
      901 tccggcaagc aatgctgtat tatgggattt tggcgatggt agtttgggca ttacacctgc
      961 gggtcaaaat gcagtgagtc ataattttcc tacaaccgga ggcgacttta caatttgtca
     1021 gactcaggtg attgacaccg gacaagatac gcttgatgtt aatgtatgct ccaaaatgat
     1081 cagtgtacca ccatgcagtg aaaacatctg tgataatatg atattggaga tgaataagac
     1141 gtgcaatggc agtgaagaat catacgtaga atttcatata aaacactatc aaaatgtagc
     1201 gacatatagg tggaattttg gtgacggtac cccatcccac acaagtatca caccagatgc
     1261 gatgcatact tacgataaat ccggctgcta taatatttgc ataactcttt atggcggcca
     1321 cggcgatttg atagctacgt gttgctatcg caagtgtatt aattgttgta gtgcggatga
     1381 ctttgatttt tattcggtac ttccaacagg atcatgcttt aaccaaaaat ttgtactgga
     1441 gaaccattgt ggtaagggaa gcaatcttaa acacatttgg gagtttgaag acgggaaggt
     1501 atattatgga gaaaatcccc cggccgaata tgtgttttca aattttgtaa atgaagacca
     1561 tgaagtatgt gtcaggcata caataatatg ttgcaatgac actataacgg ccaccaaatg
     1621 ccttccgttt acaaagggag tatttgttgg ttatgctggt aagacgacca aggctacaga
     1681 ttttgctagt tggtggggtc acccggcgat aaggatttat caaatgatag accagtattc
     1741 gaatgttccg gggctgccgc tatttatgga tgggacgtat catcatgatc tcagccatac
     1801 ttatccttct ggaacctgga acatgggata ttctgccgtt atttatgtga attcagatgc
     1861 tataataaag tacaaacctg aatcaaatcc gaatgctccg ataacctttg gtctcgaggg
     1921 tacggtcata caggacgcta agcgattcca tccccaatat ggtacatgct gtcgctggga
     1981 gggaattcgc aacaacggac cgaatagatt cgagtgggac gctgcccaaa taacagatgc
     2041 aaaaaaaatg cttgatttat ccacaaatgc aatctataat aatgagctcg ctgtattgaa
     2101 aattgaagat tgcagatttt acgagaacat ttacggtata tactctaaag gtttctcgtt
     2161 tacggttccg agcttcagca ataatgtatt tgatgggccg caccatcctc atgaaacctg
     2221 cgattgcgac gtagaagacg ctatccacct tgaaaatatt agctcgcctc ctgtggtttt
     2281 tccgaacaac aaggcaacgg ataacgaaat catggtttac caaaacgggt tgtttgccag
     2341 taattcaaat gttaaatttt gcagtttcaa catacacgac caggcgactt atgctctatt
     2401 ggacaaagac aacataggta atggtattta ttatgaaaca aataagggct tgaattattc
     2461 attgacctgc gattacatca aggtcaagaa ttttaaggca gggattaccc agcgatctgt
     2521 tgggctcggt acccaatcgc tgatggcgat agcagatgtt ccttacagta gtatatggtt
     2581 tgagaacgtc accaagggta ttgatataga tgccaccaat gtgatgctca ataccagagt
     2641 agtgggtaac aaaatcataa cgaatggcgg agacaataac cttggagtgg tagtaaagac
     2701 aaaaggagta ccggccaaga aggctcactt cgtgcagata gataacaacg acatatactg
     2761 taacggcggt ctggggacaa aagtcggtat cggcatattg aacaacaatt ttaacaagca
     2821 atatttttcg atatcaagga acaaagtcac gcataactat gatccgggtg taggtatttt
     2881 tgtgtctaac ggcagaaata caaagataac gaataattat gtcgatgtaa agacctcatc
     2941 gtcaaatgga atagagatac tggactccga tttttccaaa atatcctgta atacgttcat
     3001 gggaggtgaa ttgggtatgc gttgggctaa ttcgaaatat acattgctgt cacagaatta
     3061 taccggagac aataatgtcg ggtggagtat tgaaggagca tgcgatgctt cgaagataga
     3121 atggaacagg tttgattata cgaagagtta tagtattaat tattacgata ataccacatt
     3181 caccggaaag caggcgcacc gcaggtacaa tacctggaca tcccagaacg gaaaggaaat
     3241 taatacttta gagccactta aaaaccagat gtggtgtgac ggaagccagg cagtagggac
     3301 tgtgcacaga ccgcaagcag acgcttcggg tgtttttttt gatggtacag tatataccta
     3361 tttattacct caagatagtt gtcctgtttt acaaaatcat gataatggag ataatctgga
     3421 tggcgagggt gttttacaaa actgcattca ccgtattaat gataccctgt ctcttgctgg
     3481 atataccgat gtacagagat gcaataccga gcagtcatta ctccagtcaa taagagagaa
     3541 tcccgactgg atgttaacat cgacggtcat cagtcagttt gccggtcagc acatcaatga
     3601 tttcgtgggc aagtccgtaa cagtacaaat gatgatgtca caggtacagg ctgaagtgga
     3661 taccctgcaa tcatacctga ggccatacct tgtggaattg gattctctac tggaaataag
     3721 agcgcagctg attgaggatt tgaatacggc atcggattct gtcacttatg cggtcatcag
     3781 cgaacaattg tctgagaatg atacaatact aacacaacta cagggctata ttgacaatct
     3841 gaatgcacag aatgatatta tcatctcagc gatgcttggt gcgattgaat cattgaatgc
     3901 cagcatatca gtttccggtc ttcacgaata ttgtgaaaag aatgtcaacc agtattacct
     3961 caagacctgg cgtggcgaca gcctgagctc tggcgaaaaa gctgatttgt gggatattgc
     4021 caatacttgt tatgatgatg gagg
//
LOCUS       DAOQQY010000258         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10632_length_4035_cov_6.173869, whole genome shotgun sequence.
ACCESSION   DAOQQY010000258 DAOQQY010000000
VERSION     DAOQQY010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10632_length_4035_cov_6.173869"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1113)
                     /locus_tag="PLE23_10715"
     CDS             complement(<1..1113)
                     /locus_tag="PLE23_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HMX83780.1"
                     /translation="MARIAINLKEGNISKEEYIIGIDLGTTNSLIACVTDGKAMCLQD
                     KNGQNTLVPSVIHFDHEGKASVGKTAKDYLIEDPENTIYSVKRLLGKSFNDIENKSHF
                     INYRIIETDPTAMVRVQIRDKFYTPVELSSEILKTLKIRAEEHFNSIITKAVITVPAY
                     FNDAQRQATRDAGKLAGLDVLRIINEPTAASLAYGLDHAEGKQTIAVYDLGGGTFDIS
                     ILSLEGGIFEVLSTNGDTFLGGDNIDQDIIAFWAGKYGWNTTEIQRNRQANQQLRLMA
                     EKAKIQLSSEASFKEEYAGLIVELNRNELHTIIQPIVETTLKYCKNALNDARLTKEKI
                     DHIVMVGGSTRSPYIKDLVSQYFGKEVFDKIDPDTVV"
     gene            1225..2043
                     /locus_tag="PLE23_10720"
     CDS             1225..2043
                     /locus_tag="PLE23_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HMX83781.1"
                     /translation="MEKVHQFYEDKSKKLFIILGAFFVTNAVIAELIGVKVFSLEKTF
                     GLQPFHLSLFGLDDLSFNLTVGVLLWPVVFVMTDIINEYFGVRGVRLLSWIAAAMIAY
                     SFIMFWIGMHLVPADFWLTSHLHHDMPPEQLNSLKSKVGDLNEAFRLIFGQSSWIIIG
                     SLVAFLVGQIVDVMSFHKIKQYTGERYIWLRATGSTLISQFIDSFVVLFIAFYIGADW
                     TLKAVIAIGIMNYIYKFIIAVVLTPVIYIIHEVIEKYLGEELATKMKNQSLHYK"
     gene            2053..2991
                     /locus_tag="PLE23_10725"
     CDS             2053..2991
                     /locus_tag="PLE23_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HMX83782.1"
                     /translation="MLRKQVTRSILMVRPSNFGFNEETAVNNAFQNNAAGKTSEQIRE
                     KAIEEFDSFVSLLRSKGVDVSVYQDEPQPIKPDAVFPNNWVSFHEDGTVITYPMFAKV
                     RRIERTDKVFDLLEPHFWILDHIHLEQSEEDNIFLEGTGSMVLDRANKIAYACISPRT
                     EKTLFEDWCNIYEYAPVSFTAIDQEGKLIYHTNVMMAMGDKFVVICLESVIDDEEKSA
                     LREIFARTEKEIIEISLEQMNAFAGNMLQVQGASGQTYLVMSKTAFGSLNASQIEKIE
                     KYTEILTADINTIETYGGGSVRCMMAEIFLPEKSLA"
     gene            3048..>4035
                     /locus_tag="PLE23_10730"
     CDS             3048..>4035
                     /locus_tag="PLE23_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HMX83783.1"
                     /translation="MKKLILIMAVCLSYSITYGQIVISEIMYNDPGTADSLEYIELTN
                     ASAQAVDLTGYSFSAGVKYTFPAITLGPGQQILVAKDSVYVLAALGKAARQWKSGDAL
                     NNSGEKITLQDAGGTGVDSVSYSDSNGWPTEADGDGYSLVLCDLLSDNNLPSSWNIST
                     EKTSAVISNKNIYGTPLAPGGCINGATVSFIYSSSTVGESDPGVILDVVKKGLGVGPQ
                     SALVLLDQNNSTAQNGKDFGFQDSLLLNFDPLNELDTVTVFFPLIDDSDLEGNETIIL
                     NLVSISLTVLPSAGTATVTIVDNDSNISKKLILTGVFDAQANGAASAKGAEVY"
BASE COUNT         1151 a          786 c          818 g         1280 t
ORIGIN      
        1 gactacagtg tctggatcta tcttatcaaa aacttctttt ccaaaatatt gacttacaag
       61 gtctttaatg tacgggcttc ttgtagaacc gcctaccatc acaatgtggt ctattttttc
      121 tttggtcagt cgtgcatcgt tgagcgcatt tttacaatac ttcaaggtgg tttcaactat
      181 cggttgtatg atggtgtgaa gttcattcct gttaagttct acaatcagac cggcatattc
      241 ttccttaaaa gatgcttccg aactcagctg tatctttgct ttttcggcca tgagcctcaa
      301 ttgttggttt gcttgtcggt tacgctgaat ctctgtagtg ttccacccat atttccctgc
      361 ccagaatgct ataatgtcct ggtcaatatt atcaccaccc aagaaggtgt caccatttgt
      421 tgacaatact tcaaatattc caccttcaag tgataagatt gaaatgtcaa aagtgccacc
      481 acccaaatca tagactgcga tggtttgctt gccttcggca tggtcgagcc cataagccag
      541 gctggctgct gttggctcat tgatgatgcg tagtacatcc aagcctgcga gttttccggc
      601 atcacgggtg gcttgtcttt gcgcatcgtt gaagtacgcc ggcactgtta tgacagcttt
      661 tgtaatgatt gaattaaaat gttcttctgc tctgattttt agtgttttaa gtatttctga
      721 agataattct accggcgtgt agaacttgtc acggatctga actcttacca tggctgtggg
      781 gtctgtctct attatccggt aattgatgaa atgcgacttg ttctcaatat cgttaaaaga
      841 tttacctaat aatcttttga cagaatatat ggtattctca gggtcttcta tcaggtaatc
      901 tttagctgtt ttccctacag atgcctttcc ttcatggtcg aaatggatta cggaaggcac
      961 aagggtgttc tggccatttt tatcctgaag gcacatagcc ttaccgtccg ttacacaggc
     1021 tatcaggctg ttggtagtac caaggtcaat gccgatgata tattcttcct ttgaaatatt
     1081 tccctcttta aggtttattg ctattcttgc catgtattcg tattgaaagc acaaagttat
     1141 gaattcagtt gcacaacatg tacttagtta aattgtaata atgaaattca aaaatcatta
     1201 catttgttca ttaattttaa aaaaatggaa aaggtacacc aattttatga ggataaatca
     1261 aagaaattat tcatcatact gggtgctttt tttgttacta atgctgtcat tgctgaatta
     1321 atcggagtta aagtattttc acttgagaag acattcggtt tacagccttt ccatctgagt
     1381 ttattcgggt tagatgatct ctcttttaac cttacagttg gtgttttgct ttggcccgta
     1441 gtatttgtaa tgacggatat tatcaatgaa tattttggag tgcgtggagt aaggttgctc
     1501 tcatggatag ctgccgcaat gattgcctat tcgtttatca tgttctggat aggaatgcac
     1561 ctggtgcctg ccgatttttg gcttacttcg catcttcatc acgatatgcc acctgagcaa
     1621 ttaaattcac ttaaatccaa ggtaggtgat ctcaacgaag cattcaggct catatttggg
     1681 caaagctcct ggattatcat cggttcattg gtagcatttc ttgtaggtca gatagtggat
     1741 gtcatgtctt ttcataaaat aaaacaatat accggtgaac gctatatatg gttgcgagca
     1801 acaggttcta ctctcatcag tcagtttatt gatagttttg tggtcctttt tattgctttt
     1861 tatattggag ccgactggac acttaaagcc gttattgcca taggcatcat gaattatatc
     1921 tacaaattca tcatagcagt agtactgaca cctgtcattt acatcattca cgaagtgata
     1981 gaaaaatatc ttggtgaaga acttgcaaca aaaatgaaaa atcaatcttt acattataaa
     2041 tagtcgtaaa tcatgttaag aaaacaagta acccgtagca ttctgatggt gaggccatcc
     2101 aacttcggat tcaatgaaga gactgctgtc aacaatgcat tccaaaataa tgcggcaggc
     2161 aaaacatcag aacaaatcag agaaaaagct atcgaagaat ttgactcgtt tgtgagcctg
     2221 cttcgaagta aaggtgtaga tgtatccgta tatcaggatg aacctcaacc tattaaacct
     2281 gatgcagtat ttccaaataa ctgggtttca tttcatgaag atggcaccgt catcacttac
     2341 ccgatgtttg ccaaggtgag aagaattgag cgtacagata aggtttttga tttgctcgaa
     2401 cctcatttct ggattcttga ccatattcat ctcgaacaat cagaggaaga taatattttc
     2461 ctggaaggaa caggttcgat ggtgcttgac cgtgcaaaca aaattgctta tgcttgtatc
     2521 agcccaagga cagaaaagac cctttttgaa gactggtgca acatatacga atatgctcct
     2581 gtatctttta cagctataga tcaggaaggc aagttaattt atcatacaaa tgttatgatg
     2641 gccatgggcg ataaatttgt agtcatttgt cttgaatcgg tgatcgacga tgaagagaaa
     2701 tcggcattac gagaaatatt cgcacgtact gaaaaagaaa ttattgaaat atcactcgaa
     2761 caaatgaacg catttgccgg taacatgcta caggtacaag gtgcatcagg ccaaacttat
     2821 ctcgttatgt caaaaacagc atttgggtca cttaacgcat ctcagattga aaaaatcgaa
     2881 aaatataccg aaatattaac cgctgacatt aatactatcg aaacctacgg cggtggaagt
     2941 gtgcgttgta tgatggcaga aattttcctt cccgaaaaat ctttagcata atatggtcaa
     3001 ggattaagac aaaatatagt atttttaccc aaattaacca cattactatg aaaaaactta
     3061 tactcattat ggctgtctgt ctgagttata gcattaccta cgggcaaata gtcatttcag
     3121 aaattatgta caatgatccg ggtactgccg attcgcttga gtacatcgaa ctaaccaatg
     3181 catcggcgca ggcagtagat cttaccggat attcattttc tgccggtgtc aaatacactt
     3241 ttccggctat tacacttggt ccgggccaac aaattctcgt tgccaaggat tctgtttatg
     3301 ttctggctgc cttaggtaag gctgcaagac agtggaagtc cggtgatgca ctcaataact
     3361 ccggtgaaaa aattacattg caggatgccg gaggtaccgg cgttgattcg gtgagttatt
     3421 ctgacagcaa tggctggcct actgaagcag atggtgatgg ttattcactc gtattgtgtg
     3481 accttttgtc tgataataat ctgccttcaa gctggaatat ttctaccgaa aagacaagcg
     3541 ccgttatcag caataaaaat atttatggaa caccactcgc tccgggcggt tgtataaacg
     3601 gtgctacagt cagctttatt tattcgtcaa gtacagtagg agagtccgac cccggggtta
     3661 tacttgatgt tgttaaaaaa gggcttggtg tcggccctca atctgcactg gttttattag
     3721 accaaaacaa ttcgacagct caaaatggta aagatttcgg ttttcaggat tcccttttac
     3781 tcaatttcga cccacttaat gaactggata cagtcaccgt gttttttcca ttgattgatg
     3841 attcagacct tgaaggtaac gaaaccatca tactcaattt ggtttctatt tcattgactg
     3901 tactgccaag tgctggtact gctactgtta ccatagtgga caacgacagc aatatttcga
     3961 aaaaacttat tctcaccggt gtattcgatg cacaggctaa tggagctgct agcgcaaaag
     4021 gagctgaagt gtatg
//
LOCUS       DAOQQY010000259         4019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10702_length_4019_cov_5.306761, whole genome shotgun sequence.
ACCESSION   DAOQQY010000259 DAOQQY010000000
VERSION     DAOQQY010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10702_length_4019_cov_5.306761"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PLE23_10735"
     CDS             complement(<1..1128)
                     /locus_tag="PLE23_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83784.1"
                     /translation="MMRKFVPHIIAFALMFFSVFLLYAPYFTSGKVLNQGDTVRAYGM
                     QGEMNKIEKETGIAPLWTQSMFSGMPTFQIKPPDRGNYTRYFLNGMMLNKSMTNVPFV
                     IFMAMFCTYFLLIVLGLDWRIAVMGSVCYGLSTFYCDIAEAGHATKMMALALMPGMFA
                     GALMVLRGKYLIGASVFALFVALQVLVNHIQITFYAFIALGILVLVKLWGSIKQGQLK
                     HALIAVGVLALAGIAGLAANTSRLWPTYEYSKETIRGKSELSSNAAKGDGLDKEYAFG
                     WSYGIGESMTLLIPNYYGGGAAQTFQGTETYSRIYNNVLNDLTQRGMSADEAKRNAEQ
                     QVSSLFYTGDQPFVGVAIYFGAIICFLFVLGAFLVGGPYKIW"
     gene            1249..2028
                     /locus_tag="PLE23_10740"
     CDS             1249..2028
                     /locus_tag="PLE23_10740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX83785.1"
                     /translation="MEPGPQPCKLTVITVVFNAAELLEPTIASVRKTDFPQVEYIVID
                     GGSVDGTLDVIQDNADKIDTWVSDRDKGLYDAMNKGLNMANGQFVWFVNAGDIISSAD
                     IIQKILAAINDDTDIIYGEVMIIDAEGKELGTRSELTTQKLPEGLNWKSFRYGMRVSH
                     QGFIVRKSIAPQYELNNLSADIEWCIRCLQKSRKNVLVPGIFVHYLKGGISKKKWKKS
                     IMDRYKILAKYYGFFPNLCAHIGIMTRAVFHKILRINKISY"
     gene            2036..2413
                     /gene="crcB"
                     /locus_tag="PLE23_10745"
     CDS             2036..2413
                     /gene="crcB"
                     /locus_tag="PLE23_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HMX83786.1"
                     /translation="MSKILLLVGGGGASGAVLRYMIWRWLGVSQVGFPKATFIVNMVG
                     SLLIGVLFALLTEEKDHTTRLFAITGFLGGFTTFSSFTWESFMLFQEGKWKIALTYIV
                     ASNIAGLVFVLLGFFIVKALKNG"
     gene            complement(2899..3093)
                     /gene="rpmF"
                     /locus_tag="PLE23_10750"
     CDS             complement(2899..3093)
                     /gene="rpmF"
                     /locus_tag="PLE23_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMX83787.1"
                     /translation="MAHPKSRISKQRKRKRRTHDGVALPQLAKCPTTGEVHQLHRAYS
                     VDGNLYYRGNILVKAQEIEE"
     gene            complement(3108..3641)
                     /locus_tag="PLE23_10755"
     CDS             complement(3108..3641)
                     /locus_tag="PLE23_10755"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HMX83788.1"
                     /translation="MKEGLVAFQIPVYGIEQGVHSYHFHCGDSFFASFEKSLIENGEF
                     EVDMSLEKKSDTIELAFSIHGNIMTNCDRCTARIQLPIQGDAELMLKYAEHEKEEDEI
                     VFISRDRDTYDVSEFLHQSIELLLPMSNTYDCENDNPRPCDDRVLEVLNEQSKKTKTK
                     EEENPLWDQLKNLNLDH"
BASE COUNT         1233 a          795 c          761 g         1230 t
ORIGIN      
        1 ccagatcttg tagggcccac ccaccagaaa tgcacccagt acaaataaaa aacatataat
       61 ggcaccaaaa tatatggcaa cacccacaaa aggttgatcg ccggtgtaaa aaagggaaga
      121 cacttgctgt tcggcattgc gcttagcttc atcggcagac ataccgcgct gtgtcaaatc
      181 attgagtacg ttgttgtaaa tcctgctgta agtctccgtt ccttgaaaag tctgggcagc
      241 accaccacca tagtaattcg gaatgagcaa ggtcattgat tcgccaatac cgtagctcca
      301 accaaaggcg tactctttgt cgagaccgtc gcctttggca gcattggatg aaagctccga
      361 tttgccccga atggtttcct ttgagtattc gtatgtaggc cacagtcttg aggtattggc
      421 tgcaaggcct gctatacctg ccaacgccag tacaccaact gctataagtg catgtttgag
      481 ctgcccttgt ttaatactgc cccataattt taccaaaacg agaataccga gtgcaataaa
      541 tgcataaaat gtaatctgga tgtggtttac caggacttgt aatgctacaa aaagagcaaa
      601 aaccgatgca ccgatgagat atttacctcg caacaccatt agtgctccgg caaacatacc
      661 cggcatgaga gcaagtgcca tcatcttagt ggcatgcccg gcctctgcaa tgtcgcaata
      721 aaaagtagaa agcccgtaac ataccgaacc catgacagca atacgccagt ctaagccaag
      781 tactatgagc agaaaataag tacagaacat agccatgaag atcacaaatg gtacgttggt
      841 catactctta ttgagcatca tcccgttcag aaaatatctt gtgtaattac ctctatcagg
      901 tggcttgatc tggaaagtcg gcattcctga aaacattgat tgtgtccaaa gtggagcaat
      961 accggtttct ttctcgattt tgttcatttc tccttgcata ccataagcac gtacagtatc
     1021 accttggtta agcacttttc ctgatgtaaa atacggtgcg tacaataaaa aaacagaaaa
     1081 aaacatcagc gcaaaggcaa tgatatgagg aacaaatttt cgcatcataa gtataataaa
     1141 ttgtattttt taattgtttt tgtcttaaaa agtttcttat cagagctcac aaagttcaaa
     1201 ggtaaaaatt tttgtgtata ttcgtgtttt aaacattttt acatatttat ggagcctggc
     1261 ccccaaccct gtaaattaac agttataacg gtagtcttta atgccgccga gctgctggaa
     1321 cccaccatag caagtgtcag aaaaactgat tttcctcagg tcgaatacat cgttatcgat
     1381 ggaggttcgg ttgatggtac ccttgatgtc atacaggata atgccgacaa aatagacact
     1441 tgggtgagtg accgtgacaa aggtctttat gatgccatga acaaaggcct gaacatggca
     1501 aatggtcagt ttgtatggtt tgtcaatgcc ggtgatatca taagctctgc ggatatcatc
     1561 caaaaaatac ttgcagccat aaacgatgat accgatatca tctacggtga agtgatgata
     1621 atagatgctg aaggaaaaga actcggcacc cgatctgaac ttacaaccca gaaactaccc
     1681 gaaggactca actggaagag ttttcgctac ggcatgcggg tctcacatca gggcttcatt
     1741 gttagaaaga gcatagcgcc acaatacgag cttaacaatc tttcggctga tatcgaatgg
     1801 tgtataagat gtctgcagaa gagtagaaaa aatgtgctcg tgccaggtat ttttgtccat
     1861 tacctgaaag gcggaatctc taagaaaaaa tggaaaaaat ctattatgga tcgatacaaa
     1921 atcttggcaa aatactacgg attctttcca aacctgtgtg cacatatagg catcatgacc
     1981 agagcggtat ttcataagat attaagaatc aataaaataa gttattgaaa tccaaatgtc
     2041 aaaaatctta ttactcgttg gaggcggagg ggcctcaggt gcagtgctca gatacatgat
     2101 ctggcgttgg cttggtgttt ctcaggtcgg attcccaaaa gctacattca tagtgaacat
     2161 ggtgggaagt ttattgattg gtgttttgtt tgccttgcta acagaagaaa aagaccacac
     2221 aacgcgattg tttgccatca ccggattttt gggaggattt accacatttt caagctttac
     2281 gtgggagagt tttatgcttt ttcaggaagg aaaatggaaa attgccctta catacatcgt
     2341 tgcctctaat attgccgggt tggtcttcgt gttattaggt ttttttatag ttaaagctct
     2401 gaaaaacggg tagctcattg actttaaaca aattgaagaa aaacttgtta tttgtagtat
     2461 tgattgaata aaaataaata ctatatttgc ttaagattcg ctctatacaa gcataacctt
     2521 tgaaaacatg aaaaagtttt tcacaaaatc taatctcaag aaattcggag tggcaggttt
     2581 tctttttttc cttgctaagg gattggtgtg gctgttgctt ggttatcttg ttttgaaata
     2641 attatctgca tcttattttt cttagctaaa tttcctttgt catttcaccc aaagtgttgt
     2701 atctcatagc agagagtaga aatatgcaat ttgggcattt tcctcagtca atggattatg
     2761 ttgtacaagt tttgctaaaa cacacatatc atcagaaaaa atcaggaaaa ttgcttgcag
     2821 cggttcatga atactaaaac acaattagcc ccacaacaca aaagttatgg agctaatcgt
     2881 tatgaaaagt gcctaagact attcttctat ctcttgtgct ttaacaagga tgttgcctct
     2941 gtagtacaga ttgccatcaa cactatatgc acggtgcaat tggtgaactt cacctgttgt
     3001 tgggcatttt gccaattgtg gtaatgctac accatcatgt gtacgacgtt tgcgttttct
     3061 ctgcttcgat attctggact tcggatgtgc cataatttta tttcagttta atgatctaag
     3121 ttcaaatttt tcaattgatc ccacaatggg ttttcttcct ctttagtttt agttttcttt
     3181 gattgctcgt tcaagacttc cagtacacgg tcatcacaag gtctcggatt gtcattttca
     3241 caatcgtaag tattggacat tggcaacagc aactcgatac tctgatgcag aaattctgaa
     3301 acatcgtaag tatctcggtc acgacttatg aatactattt cgtcttcttc cttttcatgt
     3361 tctgcatact tcagcatcaa ttctgcatct ccttgtatgg gtagttgaat ccgtgctgtg
     3421 catctgtcgc agttagtcat gatattgccg tggatggaaa acgccaattc aatggtgtct
     3481 gattttttct ccagagacat atctacttcg aactcaccat tttctatcaa tgatttttca
     3541 aatgaagcaa aaaatgaatc tccacaatga aaatgatagc tgtgaactcc ctgctcaatg
     3601 ccgtaaaccg gaatctgaaa tgcaaccaat ccttctttca tcaaagaact ttttctaaaa
     3661 cgtgcgcaaa gatactatta tttttaacag gaaacaaaac ttatatagat ttttttacaa
     3721 aagtattcag tagcatcctg caacaagtat taataccaag ataagtattt tatttcatgg
     3781 cctttctatt ttgataattt tagttactaa aacagttttg cctgttttga aatcttaaaa
     3841 tatttcggaa atgctaacac ctcagcaaat ataaatataa attacacaaa aaacaatttg
     3901 aacttgaata attttttaac aaactataat attttctaac ttagcctttt ccggatattt
     3961 ataaaattta ttctgacacc ttcagcaccc gggcgtattt ccctatattc acaactgtg
//
LOCUS       DAOQQY010000260         4014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10725_length_4014_cov_5.818641, whole genome shotgun sequence.
ACCESSION   DAOQQY010000260 DAOQQY010000000
VERSION     DAOQQY010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10725_length_4014_cov_5.818641"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLE23_10760"
     CDS             complement(<1..1101)
                     /locus_tag="PLE23_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83789.1"
                     /translation="MSTKLYTGLRFLLFVSFLLGCNILFSQTVYFSEISGDSGNNDGT
                     NDGIVELAGLPGTDLSCMVISNSEWVVVLPPGTTMPASGVFLIACSEGKNLGSNPNPV
                     PGSGISCASCDFPGLPIDFDVCNPANAAYIDWSTTGFTIDNAADDDGDQLVLFAASGN
                     VLDAVSFGCGSRFLANDNTTLATATCTYGSSAGRGGGDQQYQLGMPTWNGNGNPALLP
                     VALKPGGSCAGANYFMPAINTGIYTDLTSVMNTGGKPINNSVLQGCNSSFIKIGGSWS
                     KTDHPNPGLPNNLPADKFTITGDTIQCSPGNSVVFTLEVYNWQQVEPLAIIPSGPGAG
                     NIGSFISFDGGVTRQNWSSVTTNLATGVTTLSY"
     gene            1305..1703
                     /locus_tag="PLE23_10765"
     CDS             1305..1703
                     /locus_tag="PLE23_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83790.1"
                     /translation="MKWIQILIFALICNVGFSQNAQQAKPLTIEERAAQAVEGLHKDL
                     KLSPSQQESIKKIQIKFFKQLDKAKESFMNKKGSDRNKAVKKMQALNNQREKAISKLL
                     NDEQKEIYNKKIAIGKAVKKEVMPSPDEKR"
     gene            complement(1931..2003)
                     /locus_tag="PLE23_10770"
     tRNA            complement(1931..2003)
                     /locus_tag="PLE23_10770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1968..1970),aa:Arg,seq:tct)
     gene            2112..2792
                     /gene="ung"
                     /locus_tag="PLE23_10775"
     CDS             2112..2792
                     /gene="ung"
                     /locus_tag="PLE23_10775"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HMX83791.1"
                     /translation="MPERHILLESSWKIKVGGEFEKPYFNNIRKVLLELKSKNISTYP
                     PNSKIFNAFDTTPFDQVKVVIIGQDPYHQPGQAMGLSFSVPRGIAIPASLRNIYKEIK
                     RDLGIDEPTHGDLSYWAEQGVLLLNAILTVEGGKAASHSKIGWEYFTDNVIKTLSEDR
                     EKLVFLLWGNFAKSKKNLIDMKKHYVLEAVHPSPLAGGKFIGCGHFSKTNEILISNGI
                     EPIDWRLK"
     gene            complement(2850..3353)
                     /locus_tag="PLE23_10780"
     CDS             complement(2850..3353)
                     /locus_tag="PLE23_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83792.1"
                     /translation="MKKIFTILALAIGLMFVSQNANAQEYKNGIGIRFGWGYGLSYKH
                     FFTEKGAMELVGNFRSYGAAGYKYNYIRVTGLYLVHNKISAVDGLQWYFGGGAMFQTW
                     GGDFDLFAPGAETTNIGIVGALGLDYKFANVPISITADWLPTFILSGYGDGFGGEAGG
                     LGVRYTF"
     gene            3522..>4014
                     /locus_tag="PLE23_10785"
     CDS             3522..>4014
                     /locus_tag="PLE23_10785"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMX83793.1"
                     /translation="MKKLLFAACLLLTFGLSAQDMGTKYIGGTIGFGSTKAKADGAEA
                     STNWSVSPEFGYFVAENLAVGIRLGIDGASQGDNSSSGFGADVYARKFFSASDAFRLF
                     VGANVGYATSKNTTTVGGVEFDSKENHLSAFLDLGATYDLSEKWTLIGRLGTLGFSSV
                     SDPD"
BASE COUNT         1357 a          776 c          767 g         1114 t
ORIGIN      
        1 atagcttagc gttgtcactc cggttgcgag atttgtagta acagaagacc agttctgacg
       61 agtaacgcca ccgtcgaaag atatgaaact accgatattt ccggcacctg gaccggaagg
      121 aatgattgca agtggctcaa cttgttgcca gttatatact tcaagcgtaa aaactactga
      181 attaccgggg ctacattgga tagtgtcgcc tgtgatagta aatttatctg ctggtaaatt
      241 attaggcaaa ccggggtttg ggtggtctgt ttttgaccat gatccaccaa tttttataaa
      301 gctcgaattg caaccttgta aaactgagtt attaatgggt ttaccacctg tattcatgac
      361 cgaagtaagg tctgtataaa ttcctgtatt gatggcaggc ataaaataat ttgctcctgc
      421 acaactccca ccgggcttta gtgccactgg cagcaatgca gggttaccat tcccgttcca
      481 agtaggcatg ccaagctgat attgctggtc acctcctccc ctgcctgccg aactaccata
      541 agtacatgtt gcggtagcaa gagttgtatt atcgttggct aaaaatctag aaccacaacc
      601 aaaggagaca gcatcaagta cattgccgga ggcagcaaac aacacaagct ggtcgccgtc
      661 atcatctgca gcattgtcga tggtaaaacc agttgtactc cagtcaatat aagcagcatt
      721 agccggattg cacacatcaa agtcaatcgg taagcctgga aaatcacatg atgcacaaga
      781 gatacccgaa cccggtaccg gattcggatt ggagcccagg ttttttcctt cactacaggc
      841 tatcaaaaac actccacttg caggcatggt agtaccagga ggcaacacca caacccattc
      901 cgaattgctg ataaccatac aactcaggtc tgtacccggc aaaccggcaa gctcaacgat
      961 accatcattt gtaccgtcat tgttacctga gtcacctgat atttctgaaa aataaacagt
     1021 ttgtgaaaat aatatattgc aacctaataa gaaggaaaca aataaaagaa accttaagcc
     1081 ggtgtacaac ttagttgaca tattcttgca agattataag ttaaataatt acggcttgtg
     1141 ggaggcggtt taagaggtag gaattatccg acaaaattat gttaataatt caactaaatc
     1201 aataaaaaaa tgttaaaatg tgtttaaaag ctgaaagaat aacaatattc cttgttatac
     1261 gtacattatc tcatcaaatc ttaacaaaca aaaaattgaa aaatatgaaa tggatccaga
     1321 ttttaatttt tgcacttatc tgtaatgttg gtttcagtca aaatgcccaa caggccaaac
     1381 cactgactat agaagaacgt gcagcgcagg cggtagaggg acttcacaaa gacctcaaac
     1441 ttagtccttc tcaacaagaa agcattaaaa agattcagat aaaatttttc aaacaactcg
     1501 acaaggcaaa ggaaagtttt atgaacaaga aaggcagtga ccgcaataaa gcagtaaaga
     1561 aaatgcaggc actcaataat cagagagaaa aggcaatatc caaattgcta aatgatgaac
     1621 aaaaggaaat atacaataaa aagatagcta taggcaaagc agttaagaaa gaagtaatgc
     1681 cttctccgga cgaaaaaaga tgaacagcaa tagtggcaac taataaaaaa aggcatctca
     1741 aacaggtgcc tttttttatt attcaagcaa aaattcatta acacgcttaa tttttattat
     1801 ttttcaagtg taaaagttat taaatattta gcttataaat cagaagtttg gtcaaaatat
     1861 taacaaaccc aatacgaaat tcacacaata aaaaagcact cacaaagtat gtaagtgctt
     1921 gattgataag tgattccgac tggggtcgaa ccagtaacct actgcttaga aggcagttgc
     1981 tctatccatt gagctacgga acctttgcaa aatgcgatgc aaaagtacaa tttttttctg
     2041 taaaaaaaca aatttttcac cagattcaat tgattaaata tcttagcttc gtgaattatt
     2101 aattagcagg tatgccggaa agacacatat tgctcgaatc gagctggaaa attaaagtag
     2161 gtggagagtt tgaaaagccg tatttcaaca atatcagaaa agtgctttta gaacttaaat
     2221 caaaaaatat ttcaacatac ccacccaatt caaaaatatt taatgcgttt gatacaaccc
     2281 ccttcgatca agtaaaagtg gtaatcatcg ggcaggaccc ctatcaccaa ccaggacagg
     2341 ccatgggtct gagtttttct gttccaagag gaatagcaat accagcatcg cttagaaata
     2401 tttataaaga aataaagcgg gatttaggaa tagatgaacc cacacacgga gatttaagtt
     2461 attgggctga acaaggcgtc ctattattga atgcaatttt aacggtcgaa ggcggaaaag
     2521 ccgcatcaca ttcgaagata ggttgggaat attttacaga caatgttata aaaactttaa
     2581 gcgaggatag ggaaaaactc gtctttttgc tttggggaaa ttttgccaaa tctaagaaga
     2641 atctgattga catgaaaaag cattatgtac tcgaagcagt gcatccgtct ccgcttgccg
     2701 gtggtaaatt cattggttgc gggcattttt caaagacaaa tgaaatactt atttctaatg
     2761 ggatagaacc tattgactgg cgtttaaaat aataaaggcg cagaaaaaat tctacgcctt
     2821 tacatttccg gaaatggaaa tatcgaatgt tagaatgtgt atcttacacc aagaccacca
     2881 gcctcacctc cgaagccatc accgtagcca gaaaggatga aagttggtaa ccagtcagca
     2941 gtaatactga taggaacatt agcaaatttg tagtcaagac caagcgcacc tacgatacct
     3001 atgttcgtag tttcagctcc gggagcaaat aaatcaaaat ctccgcccca agtctggaac
     3061 atagcaccac caccaaaata ccattgaaga ccgtccacag cactaatctt gttgtgtacc
     3121 aggtatagac ctgttactct gatgtaattg tatttataac ctgcggcacc gtaacttcta
     3181 aagttaccta ccaattccat tgcacccttt tctgtgaaga aatgcttgta agaaagtcca
     3241 tagccccatc caaatctgat accgatacca ttcttgtact cctgagcatt tgcgttctgg
     3301 gaaacgaaca tcaatccaat ggctaacgcc aaaattgtaa agattttttt cataatgttg
     3361 ataattttag ttgttgcaaa tctaaaacta ttccttgatt atttcaattt aaagtgaagt
     3421 ttttatttca atttttcaga aaaaaaaaaa tactcttatt ctttgcaaat atcccattac
     3481 atattacctt tgatatatta ctcactttaa acttttttat catgaaaaaa cttttatttg
     3541 cagcatgtct gctattaaca tttggtctta gtgctcagga tatgggtacc aagtacatcg
     3601 gtggcacaat tggttttggt tcaaccaaag caaaagctga tggagcagaa gctagcacaa
     3661 attggtctgt tagtccggaa tttggttatt tcgttgctga gaatctggca gtcggtatcc
     3721 gtttgggtat cgatggtgca tctcaaggcg ataactcaag ctcaggattt ggagctgatg
     3781 tgtatgcgag aaaatttttc tcagcatctg atgctttcag actatttgta ggggctaatg
     3841 taggttatgc tacgtcaaag aacactacta ctgtaggtgg tgttgaattt gattctaaag
     3901 aaaatcacct aagtgcattc cttgatcttg gagctaccta tgatctgtct gaaaaatgga
     3961 cacttatcgg cagacttggt acactaggtt tttcaagtgt atctgaccct gata
//
LOCUS       DAOQQY010000261         3997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10814_length_3997_cov_5.161847, whole genome shotgun sequence.
ACCESSION   DAOQQY010000261 DAOQQY010000000
VERSION     DAOQQY010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10814_length_3997_cov_5.161847"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLE23_10790"
     CDS             complement(<1..533)
                     /locus_tag="PLE23_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HMX83794.1"
                     /translation="MKRIAFNGMGRIGRLTLRNLTNEPDIEIVAINDLTDSSTIAHLL
                     KYDTAHGHLPNHITGDEEYLYFDDKKIKVFAIKNPLELPWKELAIDVVIECTGVFTDF
                     EKAMMHHTAGARKVIISAPAKGADVQTIVLGVNDEEIDADDIVFSNASCTTNCLAPVI
                     KILDQQFGFVEGMMSTT"
     gene            689..763
                     /locus_tag="PLE23_10795"
     tRNA            689..763
                     /locus_tag="PLE23_10795"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:722..724,aa:Glu,seq:ttc)
     gene            complement(970..1686)
                     /locus_tag="PLE23_10800"
     CDS             complement(970..1686)
                     /locus_tag="PLE23_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83795.1"
                     /translation="MPFNLEYIKEKLTPWPILVKRKTSELLWASIFNNTIVGSTWLKD
                     QAFSPGRWAVGYPALYLLYRIYNEIQPRSILEFGLGETSKLLCQYAQSFKCVYTIVEH
                     DEKWVSFFTGRNPVDSSCIFRTDISRVPYKHGKPYVYDGLTERIKGKKFDLIVVDGPF
                     GTSKYSRTDVLDIVKNNHLEEKFIIFMDDYQRSGEKNTISDLIQILNEKGIAFEKAVY
                     KGEKHCLILCSPEYKFLCTL"
     gene            complement(1820..3037)
                     /locus_tag="PLE23_10805"
     CDS             complement(1820..3037)
                     /locus_tag="PLE23_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83796.1"
                     /translation="MKGIFTAIFTLLFAYCGLSQSFRSILFHTSSTDNGLEFIEITGT
                     PGMPLTGMHFINIIGKANSTEAGVMRTVIDLGSYSLGANGLLLIRDASTEIAPGPDPE
                     TEVAVENFKPNLFNGTNTFVIVSGFTGNEGDDIDTNDDGTADITPWSNVVTSISTKQG
                     VNSKLIYADDFGGTYIQNPYGKGFLFYYNNQWNFANMTGDIMGMSVSDGTYSGRQITP
                     GKTAAVLPVDLSLFVLRKSNKDVMIEWQTASEQNNAGFDVEKSNDGSHFESIAKINGA
                     GDSQQFLNYSYTDSKPFNGINYYRLKQTDTDGGFTYSPVKSIRISQSGSVSLYPAVAV
                     GQLTLSFSDALTDDTKLDIVDLGSGRIAHTSIIAAKTKEYNLSTTALPVGTYSVVLNG
                     GAFSESLKFVVAK"
     gene            3483..>3997
                     /locus_tag="PLE23_10810"
     CDS             3483..>3997
                     /locus_tag="PLE23_10810"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMX83797.1"
                     /translation="MKKSDPNRVVDDLVEMPDEFYEHFSIVCDPKQTPIRIDKFLMDR
                     VERVSRNKLQDAIRAGNILVDGKMVKANCKVKPGNTITLVLPTPPDDEYEVKPEYIPL
                     DIVYEDEDVMVINKPVGLVVHPGVGNKSGTLVNGLTYYFQNKGLPLKEGNTPDRAGLV
                     HRIDKDTSGLI"
BASE COUNT         1151 a          784 c          843 g         1219 t
ORIGIN      
        1 tgcgtggtgg acatcatccc ttctacaaag ccaaactgtt ggtcaagtat ctttatgacc
       61 ggagcaagac agttggtcgt acagctcgca ttcgaaaata caatgtcatc tgcatctatc
      121 tcttcgtcat tgacacctaa cacgatggtc tgcacgtctg ctcctttagc cggggcagaa
      181 atgatgacct ttcgggcacc ggccgtatga tgcatcatag ccttttcaaa atccgtaaag
      241 acaccggtac attctattac tacatctatg gcgagttctt tccagggtag ttcgagggga
      301 ttcttgattg caaatacctt tattttcttg tcatcaaaat ataggtattc ttcatcgcct
      361 gttatatggt ttggaaggtg accgtgggca gtatcatatt tgagcaggtg tgcaatggtg
      421 ctgctatctg ttaggtcgtt gatggctaca atttctatat ccggctcatt ggtgaggttt
      481 ctgagtgtaa gtctgcctat tctacccatt ccattaaatg ctatccgctt catcacattg
      541 tattagtaag aaaataaaac gcaataagta tataattagt tcaacaaagc caacgaaagt
      601 cgcatttttg aaaaatattt aatttttttt taaaaatatt tggatacaaa aaaagaatag
      661 tcatatattt gtgtcgcttt tggaaaaagg cccgttcgtc tatcggttag gactcaagat
      721 tttcattctt gcaagagggg ttcgattccc ctacgggcta ccaaagaggc tccattccgg
      781 atgcctcttt tttatttcta gtcttttctc tttgttaaaa tcctaatttt atagggcgct
      841 attgttgcat ggagtgcact tgtccatctc tgcctgaata aattgtatct tagtttccag
      901 ttagtgtaac taaaaatgtt ctttcttagg tgtttcttgg tctgatgttg tttggataat
      961 ttgaatattt taaagcgtac acaggaattt gtattccgga gagcaaagaa taagacaatg
     1021 tttttcgcct ttatacactg ccttttcaaa tgcaattcct ttttcattca atatttgtat
     1081 caggtctgaa atggtgtttt tttcacctga gcgctgatag tcatccatga aaatgatgaa
     1141 tttttcttca agatgattat tttttacaat gtcaagtaca tccgtccgtg aatatttgga
     1201 tgtgccaaaa ggaccatcca ctacaataag gtcaaacttt tttcccttta tcctctccgt
     1261 caatccatca taaacataag gcttcccatg cttgtagggc actctcgaaa tgtcggtcct
     1321 gaatatacaa gaggagtcaa caggatttct gcctgtaaaa aaagataccc atttttcatc
     1381 atgttcgact atggtatata cgcacttaaa actttgagca tactggcata aaagctttga
     1441 tgtctcgcca agaccaaact ccaaaatact gcgaggctga atctcattgt atatccggta
     1501 cagcaaataa agtgctgggt agcccacagc ccaacgcccg ggactaaatg cctggtcctt
     1561 aagccaagta gaacctacta tggtattatt aaaaatacta gcccacagta gctcactggt
     1621 ttttctcttt accaatatgg gccaaggtgt tagtttttct ttgatatact ctaaattaaa
     1681 gggcattttt cggattttgc tgctaaatta taaattattc agataatagg tgaatattga
     1741 ctgtaacaaa gccatagacc tacaaactaa attgctataa aaccggaaat accgaaatac
     1801 aaattatgcc caaacatctc tatttggcaa caacaaactt taggctctca gagaatgctc
     1861 ctccgttcag tacaacactg taggtgccga caggaagtgc agttgttgac agattgtatt
     1921 ctttggtttt ggctgcaata atcgaggtgt gcgcaatcct tcctgaacca aggtcaacga
     1981 tatcgagctt ggtgtcatct gtaagtgcat ctgaaaaact tagtgtaagc tggccaacgg
     2041 ctacggcagg atagagcgat acactacccg actgtgagat gcgaatgctc ttaacagggc
     2101 tataagtaaa gccaccatct gtatcagtct gttttagtct gtagtaattg attccgttga
     2161 atggcttgct atctgtatat gaataattaa gaaattgttg agaatcgcct gcaccgttaa
     2221 tcttcgctat gctttcgaag tggctaccgt cgtttgattt ttctacatca aatcctgcat
     2281 tgttctgctc agatgctgtt tgccattcta tcataacatc tttattggat tttctaagaa
     2341 caaacaaact taggtcaacc ggaagcactg cggcagtctt gccgggtgtt atctgtctgc
     2401 ctgaatacgt accgtctgac acactcatac ccatgatatc acctgtcata ttggcaaagt
     2461 tccactgatt gttatagtaa aacaggaagc ctttgccata agggttttga atgtaggtgc
     2521 cgccgaagtc atcagcatat atgagcttgc tgttaacacc ttgttttgtg gaaatagagg
     2581 taacgacatt tgaccatggt gtgatatctg cagtaccatc gtcattggta tcaatgtcat
     2641 ctccttcatt acctgtgaag ccggaaacta tgacgaaagt attggtgcca ttgaacaaat
     2701 tgggtttgaa gttttcaact gcaacctcag tttcaggatc cgggcctgga gcaatctctg
     2761 ttgaagcatc gcgtataagt aatagtccgt tagcacccaa tgagtaggag ccaaggtcga
     2821 taactgtacg catcacacct gcttcagtac tattcgcttt tccaataata ttgataaaat
     2881 gcatacctgt cagaggcata ccgggcgttc cggttatttc aataaattcg agtccattat
     2941 ctgtagaaga ggtatgaaac aaaatcgacc tgaaactctg actgagacca cagtatgcaa
     3001 ataataatgt aaaaatcgct gtaaaaatac ctttcatgat ataatttttg ggttacaatg
     3061 ataaatttat gaatattcgt tagcgaaaat tatagaaaat actgtaccaa atatgtgaat
     3121 atatactgtt gtgttgaaaa ataattttgt ttaaaagact tgcaactcta ggcatgtgtt
     3181 taatacggat aaggtaatag actctgttct gcagaaccaa tcatttcaca tccgacaact
     3241 ccttaccatt taccactcaa catttaccac tcaacattta ccactcaaca tttaccactt
     3301 accagctgcg tgaaggatag aaggcggcac ggagtaagcc ggatagcctg acccgacagg
     3361 ggcacgccca agcaaatatg gcagcagctg tatatgaagt actgttattt ggtggtgtta
     3421 tgtggattgc tgtatgcctt gtcagaaatt tgtcgtattt tgtgccgttt ttttaataga
     3481 atatgaagaa atctgaccca aatagagtag tggatgatct ggtggagatg ccggacgaat
     3541 tttacgagca tttcagcatc gtctgcgatc cgaaacaaac gccaatacgc atcgataagt
     3601 ttttgatgga tagggtggag agggtgtcgc gcaataagct gcaggatgca atacgtgcag
     3661 gaaacatact ggtggatggg aaaatggtga aggccaactg caaggtgaaa cccggtaata
     3721 ccataacact ggtgttgcca acaccgccgg atgacgagta tgaggtgaag ccggaataca
     3781 taccgttgga cattgtgtat gaggatgaag atgtaatggt gatcaataag ccggtgggtc
     3841 tggtggtgca tccgggagtg ggtaataaga gtgggacgtt ggtgaatggc ttgacatatt
     3901 attttcagaa taagggattg ccgctgaagg agggaaatac gcctgaccgt gcagggctgg
     3961 tacaccggat agacaaagat acgagtggtt tgatatt
//
LOCUS       DAOQQY010000262         3967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10942_length_3967_cov_4.931237, whole genome shotgun sequence.
ACCESSION   DAOQQY010000262 DAOQQY010000000
VERSION     DAOQQY010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10942_length_3967_cov_4.931237"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PLE23_10815"
     CDS             <1..327
                     /locus_tag="PLE23_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX83798.1"
                     /translation="DYQAHILTDKCMYELDLLTQILKSRPGVKISIRVYTDARGNNEY
                     NTRLTKEIAEKMVSYIAGKGINRSRLLGLGMGSEQTNQPNNMDKDHIENHYLASRKAE
                     IQILSM"
     gene            complement(335..1525)
                     /gene="sucC"
                     /locus_tag="PLE23_10820"
     CDS             complement(335..1525)
                     /gene="sucC"
                     /locus_tag="PLE23_10820"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMX83799.1"
                     /translation="MDLHEYQGKELLKSFGVRIQEGIVAHTPEEAYHAAIELKEKYNS
                     GWCVVKAQVHAGGRGKGGGVKLAKSPDEAKMHASNIIGMHLITPQTSAEGKLVRKVLI
                     AQDVYYPGETETKEFYVSVLTDRGRQCNVIIYSTEGGMNIEEVAEKTPHLVHKEMIDP
                     AYGLRAFQLNKIAFNLGLSGTAFKEMRSFISNIYNAFVSSDASLIEINPCLKSSDNLI
                     IAADTKVSLDDNGLFRHADLEAMRDTDEEDPIEVEAKEHDLNYVKLDGNVGCMVNGAG
                     LAMATMDIIKLSGGDPANFLDVGGTADANRVEKAFRIILKDPNVKAILINIFGGIVRC
                     DRVAQGVVDAYKSIGDIPVPIIVRLQGTNADEGKKIIDESGLKVHSAILLKEAADLIS
                     KVLK"
     gene            2398..2769
                     /locus_tag="PLE23_10825"
     CDS             2398..2769
                     /locus_tag="PLE23_10825"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HMX83800.1"
                     /translation="MEYNKLTDEEERVILHKGTEMPFTNEYNNHFKPGKYICRQCNAV
                     LYHSDDKFHSGCGWPSFDDTVSNAVTEVADADGRRTEIICTNCKGHLGHVFRGERFTE
                     KNTRHCVNSLSIRFIPSTHIS"
     gene            2850..3416
                     /gene="msrA"
                     /locus_tag="PLE23_10830"
     CDS             2850..3416
                     /gene="msrA"
                     /locus_tag="PLE23_10830"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMX83801.1"
                     /translation="MESNLVQNSLPQGGTESIVLGGGCFWCIEAVLKELKGVSKVVSG
                     YAGGTVRNPTYREVSAGLTGHAEVVQVSFDPKVITLEELLEVFWTLHDPTTLNRQGAD
                     VGTQYRSVIFFSNPAQKNAAEKVKKEVAAKIWDDPVITEISPLKEFYPAEDYHQNYYE
                     LNKTQPYCQIVINPKVKKLKEKFGTKLK"
     gene            complement(3426..>3967)
                     /locus_tag="PLE23_10835"
     CDS             complement(3426..>3967)
                     /locus_tag="PLE23_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol 3-phosphate dehydrogenase"
                     /protein_id="HMX83802.1"
                     /translation="FDEVIQIGQLALNSHKFHLFGSNGMLGAELAGALKNIFAIGSGI
                     LNGVGMGRNIQAMLISRGLIEMIYFGKAMGASPQDFLGTAGVGDLIATATSTQSRNYQ
                     FGTRLGKGETFDDIMQTMPELAEGVRTLKISQQLARHYKLHVPITSMLHSIVYNKLDI
                     DRAINLLMNYPFNQDVDFL"
BASE COUNT         1247 a          807 c          765 g         1148 t
ORIGIN      
        1 gactatcagg cacatatact tacagacaaa tgtatgtatg aacttgatct attgacgcaa
       61 atacttaagt caaggccagg agtcaaaatc tctatccgcg tatatacaga tgcacgtggc
      121 aacaacgagt ataatacacg actcacaaaa gagatagctg aaaaaatggt cagttatatc
      181 gcaggcaaag gtatcaaccg gtctagattg ttaggacttg gtatgggtag tgagcaaaca
      241 aaccagccaa acaacatgga taaagaccat atagaaaacc attaccttgc cagtcgcaaa
      301 gcagagatac agatactgag tatgtaagtc actattactt aagcaccttc gaaataaggt
      361 cagcggcttc tttaaggaga atagctgagt gtactttcag gcccgattca tcaatgattt
      421 ttttaccttc atcagcattc gttccttgaa gtcttacaat aatcggtacc ggtatatcgc
      481 caattgactt atatgcatcg actacgcctt gagcaacgcg atcacaacgt acgataccac
      541 caaatatatt gataagaata gctttaacgt taggatcttt gagaatgatt ctgaaagcct
      601 tctccaccct attggcatcg gctgtacctc cgacatcaag aaaattggca ggatcgccac
      661 ctgaaagttt aataatatcc attgtagcca tagcaaggcc ggcaccattg accatacagc
      721 cgacgttacc atcaagtttt acgtaattga ggtcatgctc tttggcttcc acttctatcg
      781 ggtcttcttc atccgtatca cgcattgctt ccaggtcagc atgtctgaac aagccgttgt
      841 catcaaggct tacctttgta tcagcagcaa tgatgaggtt gtctgaagat tttaggcaag
      901 ggttaatttc gatgaggctt gcatccgaac tgacaaacgc attgtatata ttagaaataa
      961 aagagcgcat ttctttaaag gcagtgccac tcaggccaag attaaaggca attttgttaa
     1021 gttgaaatgc tctaagccca taggcagggt ctatcatttc tttatgtacc aggtgtggtg
     1081 ttttctcagc tacttcttcg atgttcattc caccttcagt tgaataaata atgacattac
     1141 attgtctgcc ccggtcggtg agtacactta cgtaaaattc ctttgtttcg gtttctcccg
     1201 gataataaac atcctgagca atgagaactt tgcgtaccag ttttccttca gcggatgttt
     1261 gaggcgtaat caggtgcata ccaatgatat tggatgcgtg cattttggct tcatcggggc
     1321 ttttggccag cttgacacca ccccctttac cacgaccgcc tgcatgtacc tgtgctttaa
     1381 ccacacacca gccggagtta tatttttctt ttaattcgat tgctgcatga taagcctctt
     1441 ccggcgtatg agcgacaatt ccttcctgaa tgcgaacgcc gaaagacttc aataattctt
     1501 ttccctgata ttcgtgtaaa tccatgataa gtttagcttg atttgtattt caaaaattgc
     1561 gccccaaaag taggaaaaaa tttgattgta cccaaaggaa ttttcagctt gttggataaa
     1621 gacgttcatt ttcataaaaa aaagccgcga gaatatgaat aagcttatgt aaaacagtgt
     1681 agaatgccgg aacaaaaggc cgtaaaaaac aagaagccgc ctttaggtag aggcgacttc
     1741 ttgtgacaaa ttataatccc atgaacatat ccataatcag tgtgacatta cataccggag
     1801 tataatacta taacctcctc tttaccgata tattttaata tatatatgtg tttaacaaat
     1861 ccataacact ccggtactca atgtcaattt taggtttttg ggtttggttt tctttactta
     1921 ccacttgcat tattgcaaag gttcgttatt tgacggatga aatattaacc ctttttgttt
     1981 cagtgactaa ctttataatc acattgacaa tacaaagatg cggcactagt ggctgcttac
     2041 aaaaaacaaa aaaatcaagt tgtgaattac attatattca catacgtaat aattatttgc
     2101 ttatcttatt attatacaac tgtttatact tcattaaaat tccaaatata ttatattttg
     2161 tgaataatta aaatacattt ttttcctatt ttcgaccaat atttttatta tttttatgaa
     2221 aattcacaaa aattcctata aaaaattcat acacatacca tttgcgtagc aaaaaaactg
     2281 atgtatttga atgtttcaaa ccttatttat ggatattcaa atggcctggt ttagttggca
     2341 accggaaact tttaagacct ttagcttgtt gtgtttacag accaacaatc ggaatcaatg
     2401 gaatataaca aactaacaga tgaagaagag agggttattc tccacaaagg gactgaaatg
     2461 ccttttacca atgaatacaa taatcatttc aagccgggga aatatatatg cagacaatgt
     2521 aatgctgtac tatatcattc ggatgacaaa tttcattcag gttgtggctg gccttcgttt
     2581 gatgacacag tgtctaatgc tgtcaccgaa gtagctgatg ccgacggcag acgtacagaa
     2641 ataatatgca cgaactgcaa agggcatctg ggtcatgtat tcagaggtga aagatttacc
     2701 gaaaaaaata ccagacactg cgtaaattcg ttatcaataa gatttatacc aagtacacat
     2761 atatcatgaa cagattattt ttcattcccg cattattttt tttcacgcta ttgtcatgtg
     2821 gcaacaaacc atctgaaaac aaaaatctta tggaatcaaa cctcgtacaa aacagtttac
     2881 cgcagggtgg tacggagtct atcgtactag gtggtggatg cttttggtgt attgaagcag
     2941 tattgaagga actaaaggga gtgtcaaaag tggtgtcggg ctatgccggt ggcacggtca
     3001 ggaacccgac ttacagagaa gtaagtgcag gtctcaccgg tcatgctgaa gtagtgcagg
     3061 taagttttga cccaaaagtc atcactcttg aagaattgct tgaggtattc tggacattgc
     3121 acgacccaac aacacttaac agacagggtg cagacgtagg cacacaatac agatctgtaa
     3181 tattttttag caatccggcc caaaaaaacg cagccgaaaa ggtaaagaag gaagtagcag
     3241 caaagatctg ggatgatcct gtaatcaccg aaattagccc attgaaagaa ttttatccgg
     3301 cggaagacta ccatcagaat tattatgaac tcaacaagac ccaaccatac tgccagatag
     3361 tgattaaccc gaaggtaaaa aaactgaaag aaaagtttgg tacgaaatta aaataaccga
     3421 agtcttcaaa ggaagtcaac atcctggttg aacggatagt tcataagcaa attgatagcc
     3481 ctgtctatat ccagcttgtt atatacaata ctgtgaagca tggaggtaat gggtacatgc
     3541 agcttgtaat ggcgggctag ttgctgggat atcttcaacg tacgaacacc ttcggcgagt
     3601 tcgggcattg tttgcataat atcgtcaaac gtctctcctt ttccaagacg agtaccaaac
     3661 tgataattgc ggctttgtgt actggtagct gtagcaatta ggtctcccac acctgctgta
     3721 cccagaaaat cctgaggact tgctcccata gccttaccga agtaaatcat ttctatcaac
     3781 cctcgtgata tgagcatcgc ctggatattt cggcccatgc ctaccccatt aagaataccc
     3841 gacccgatgg caaaaatatt tttaagtgca cctgccagct cggcgcccag catcccgttt
     3901 gatccaaaga ggtgaaactt atggctattc aaagccagct gtcctatttg gatgacttca
     3961 tcaaaat
//
LOCUS       DAOQQY010000263         3958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_10989_length_3958_cov_6.647451, whole genome shotgun sequence.
ACCESSION   DAOQQY010000263 DAOQQY010000000
VERSION     DAOQQY010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10989_length_3958_cov_6.647451"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PLE23_10840"
     CDS             <1..641
                     /locus_tag="PLE23_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83803.1"
                     /translation="ILNNPNIKRQVLPNAGKFIDNILPDSRPALKDYARYVPTEDVMV
                     KPPVRPCQIEFEGEDEFMGKRRKDHAAQVLFFYTPAPLTKNYPDSEYLTCKAALSVVS
                     GGSMFLNLYFYIAVNDAGRLFGGFEKGSIINLKFLDGDNIAIINNRNDPGLYDPVSKT
                     MMFRAQCNLGAAAQDKISSKSLDKIRVTWQTGYEDYDVFDVDSLMRQAKCIQ"
     gene            646..1227
                     /locus_tag="PLE23_10845"
     CDS             646..1227
                     /locus_tag="PLE23_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-(ms[2]io[6]A)-hydroxylase"
                     /protein_id="HMX83804.1"
                     /translation="MLGLKLTTDPRWVNLAEKSLEDILTDHAYCEQKAATSAITLIQI
                     FPEKEEMVKELAPIVTEEWAHFRMVLNELEKRNLKLGRQRKDEYVNNLQKFQLKGGNR
                     KNWLTEKLLTCALIEARSCERFRLLSLELADESLRNFYHKLMISEAGHYRLFLDLAKK
                     YNDEAYVKKRLQEYLDYEAEMIATLELRGDRVH"
     gene            1280..1879
                     /locus_tag="PLE23_10850"
     CDS             1280..1879
                     /locus_tag="PLE23_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83805.1"
                     /translation="MATIILFSCGQFTDPRKEYAGDYTVRIELDKKLISDEAVQDTIN
                     SKMAELKEKLAKAETEMKDSFNLESIDTTTPEGRMEYAAKSFAKGMTGLLSPLGELGK
                     SFGEMGAGYAKGGIAMLNDLVGKMKFNVSLNANGTINTDYDSVDVLGTRNSTWNIEDN
                     KFRLISKDNKIQSFDIVSKKEDGFVLRKDENTKLIFEKK"
     gene            complement(2041..3441)
                     /locus_tag="PLE23_10855"
     CDS             complement(2041..3441)
                     /locus_tag="PLE23_10855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMX83806.1"
                     /translation="MAAVDPFLQDWDERFHALVAKNLIHHPLTPMLRLDPIMAFKIED
                     WCCNHIWVHKQPLFLWQMALSMKLFGVNEVAMRLPSVIMGTISIYFVYDLANRWIKNI
                     DISFVAALLFAVSNYQLELTSGRFPLEQNDVAFAFYVTASIWAFVRYLQSGRTFKWAI
                     FIGIFVGCAVLNKWLTGILIYGGWGLYELLSNKGKIHFKPWKLFVVSVFTSLLVFVPW
                     QLYIMHYFPAESSVMYKHNSKHIFEALEGHGGTIWFHFAEMSNIYGVSLLLFILLGAY
                     AILSDKQVDKKLSGSLIAMVLVIYSFFSFVVKTKMPAFTFPVNSIIWILIASGIITSF
                     VRVSNEQKPYILILWIVLASIITLKPWEISDKRSHDNQPRNAKIHNTQIFKKLDVAGK
                     LKNRVILNCKSFEDVELMFYKDVNAYHWFPDESTLDSLMKKGHTFAAFQSHHNQQLPD
                     YIQHNPAIKIINEALE"
BASE COUNT         1371 a          750 c          751 g         1086 t
ORIGIN      
        1 ctatattgaa caacccgaac ataaaaagac aggtattacc aaatgccggt aaatttattg
       61 acaacatact acccgattca agacccgctc tcaaagatta cgcaaggtat gtgccaactg
      121 aagatgtaat ggtaaaaccg ccggtaaggc catgccagat cgaatttgaa ggcgaagatg
      181 agtttatggg aaagcgcagg aaagaccatg ctgctcaggt attgtttttt tacactcctg
      241 cacctttgac aaaaaattac ccggacagcg aatatcttac ctgcaaggcg gctctatcag
      301 tagtgagtgg gggtagtatg tttcttaatc tgtattttta tatagcagtg aatgatgcag
      361 gaaggctgtt tggcggattc gaaaagggaa gtattatcaa cctcaaattt ctggatggag
      421 acaatatagc catcattaac aacagaaatg accccgggct ctacgatcct gtaagcaaaa
      481 cgatgatgtt cagggcgcag tgtaatcttg gtgctgctgc acaagacaaa atatcaagta
      541 aatctttaga taaaatcaga gttacctggc aaaccggcta cgaagactac gacgtgttcg
      601 atgtcgattc gttgatgcgc caagcaaaat gtattcaata gtattatgtt aggattaaaa
      661 ttgacaaccg acccgcgttg ggttaatctg gcagaaaaaa gccttgaaga tatactcaca
      721 gaccatgcat actgtgaaca aaaagctgct acttcggcca ttacactcat tcagatattt
      781 cctgaaaaag aagaaatggt aaaagaactg gcacctatag ttaccgaaga gtgggctcac
      841 ttcagaatgg tactgaacga actcgaaaaa cgcaatttga aactcggcag gcaacgcaaa
      901 gacgaatatg tcaataatct tcagaaattt cagctcaaag gtggcaaccg caaaaactgg
      961 ctgaccgaaa aactgctgac ctgcgccctg atagaagcaa gaagctgtga gcgattcagg
     1021 ttgctgtcac ttgagctggc agatgagtcg ctccgcaatt tttaccacaa actcatgata
     1081 tcagaagccg gccactatag attatttctt gatctggcaa agaaatacaa cgatgaagca
     1141 tacgttaaga aaagattgca ggaatatctg gattatgaag cagaaatgat agctacgttg
     1201 gaactaagag gcgaccgcgt gcattaatat taaaagacaa gaaatattta tgaaacatct
     1261 taaaacaacc ctcccattta tggctacaat tattctattc tcctgcggac aattcaccga
     1321 cccaagaaaa gaatatgccg gtgactatac ggtaagaata gaactcgaca aaaaattaat
     1381 atcagatgaa gccgtacagg atacgatcaa ctcaaaaatg gcagaactga aggaaaaact
     1441 agcaaaggca gagactgaga tgaaagacag cttcaacctc gaaagtatag acaccaccac
     1501 acctgaaggc agaatggagt atgcagccaa atcatttgcc aaaggtatga caggcttatt
     1561 gagcccattg ggtgagctag gtaagtcatt tggtgaaatg ggtgccggat atgcaaaagg
     1621 cggtatcgct atgttaaacg atttggttgg caagatgaaa tttaatgtat cgctcaatgc
     1681 taacggaacc atcaatacag attatgacag cgtcgatgta ctcggcacac gtaactcaac
     1741 ctggaatatt gaagacaaca aatttcgact catctctaaa gataataaga tacaaagttt
     1801 tgacattgta agcaaaaaag aagacgggtt tgtattgcga aaggacgaaa ataccaaact
     1861 gatcttcgag aaaaaataat attcagcaac acattggaca attgcggccc aacgtgttgg
     1921 aagaaattat tcgcaaaatc gcatctgcct atcaaaaaca atcaaatttt ccggaaaaaa
     1981 tcagctttat gtttgtgaag caagggctaa agtatggcaa tagctgagtt gttaacattt
     2041 ttattctaaa gcctcattaa taattttaat ggccgggtta tgttgaatgt aatcaggcag
     2101 ttgttgatta tgatgactct gaaaagcagc aaatgtatga cctttcttca ttaggctatc
     2161 cagagtagat tcatcaggaa accaatgata agcatttaca tctttgtaaa acattaattc
     2221 tacatcttca aaactcttgc agttcagaat tactctgttt ttcaattttc cggcaacatc
     2281 taatttttta aatatttggg tattgtgaat tttagcattt cttggctgat tgtcatgaga
     2341 ccttttgtca gatatttccc agggtttcaa tgttataatt gaagccaata caatccatag
     2401 tattaatata tatggttttt gctcattaga aactctaaca aagctcgtta ttattcctga
     2461 ggcaatgaga atccagatga tggaatttac cggaaatgta aatgcaggca tttttgtttt
     2521 cacaacaaag gaaaaaaatg aataaataac cagcaccatc gctattagtg aacccgaaag
     2581 ctttttatca acttgtttgt cagacagaat agcgtatgcg cccaataata taaaaagcag
     2641 tagagatact ccataaatat tgctcatttc tgcaaaatga aaccagattg tgccaccatg
     2701 tccttctaaa gcctcaaata tatgtttgct gttatgttta tacatgacgc tactttcggc
     2761 tggaaaataa tgcatgatat acaattgcca gggaacaaac accagaagac ttgtaaatac
     2821 agatacaacg aataacttcc atggtttgaa gtgaatttta cctttgttag ataacaactc
     2881 gtacagaccc cacccaccat agatcaaaat tcctgtcagc catttgttca atacagcaca
     2941 accgacaaaa ataccaatga atattgccca cttgaatgtt cttccagatt gtagatatct
     3001 aacaaaagcc cagatactcg ccgttacata aaatgcaaaa gctacatcat tttgttcaag
     3061 aggaaatctg ccggaagtaa gctcaagctg gtaattggaa actgcaaata acaatgctgc
     3121 cacaaatgaa atatcaatgt ttttaatcca tctattggct aagtcataga caaaatatat
     3181 tgaaatagtt cccatgatta ctgaagggag tctcatagca acttcattga caccaaaaag
     3241 tttcatggac aaggccatct gccaaaggaa taagggctgc ttgtgtaccc agatgtggtt
     3301 acaacaccaa tcctcaatct taaatgccat aataggatca agcctcaaca tgggcgttaa
     3361 agggtgatgt attaaatttt tagctaccag agcatgaaag cgctcgtccc agtcttgcaa
     3421 aaacgggtca actgctgcca taaataaccg gagtaaaaag ccggttatca gtagtaaaaa
     3481 aagggcactc tgcggtcttt tctgattaaa atggatataa gtactcaata ttaccaataa
     3541 gataactaaa acgagaacca catttacctg agtcgagtaa aatatcaatt tcgatgttgt
     3601 tttgtatttg actaaaaacc tgcgtaaccg ttaaaatgca aagttacgac agatgatgtc
     3661 aaatttttta agaatcaatt gtttaatcga ccatcttcga atgaattaat atagcctttt
     3721 ccaaaaaaag tttacaggtt aaaaattaaa tgctgttatc agcttacagt tgttttttag
     3781 tcttgacatt ggtttacttt tttgtctatt tttacacaat ttgtgtaaac ctattctagg
     3841 acaagacagt tggctgtgat accaatgtta cagattttct cacatctcca ttctcacatc
     3901 tcacttttaa tttttaattg aatgcgcgta ttctatggta aaaaccaagc actcacat
//
LOCUS       DAOQQY010000264         3951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11017_length_3951_cov_4.411704, whole genome shotgun sequence.
ACCESSION   DAOQQY010000264 DAOQQY010000000
VERSION     DAOQQY010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11017_length_3951_cov_4.411704"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLE23_10860"
     CDS             complement(<1..458)
                     /locus_tag="PLE23_10860"
                     /inference="COORDINATES: protein motif:HMM:NF019057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HMX83807.1"
                     /translation="MSDQNHRPDDYLPCERYLNGEYDEHGFNRFSENDKFYFSFLENG
                     NVFLRSEGYGTEAARENGIASVKTNMTEDKNYQTRLLPDGKWVLSLKAANHQEIARSC
                     AVEAEEEAKAFLPSERIKAAALAAQKAATSANEADDYMICREYEEKIGSVS"
     gene            complement(798..1391)
                     /locus_tag="PLE23_10865"
     CDS             complement(798..1391)
                     /locus_tag="PLE23_10865"
                     /inference="COORDINATES: protein motif:HMM:NF023521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3575 domain-containing protein"
                     /protein_id="HMX83808.1"
                     /translation="MKKIILGVLLLTGAYSINAQVDVSLNPISLLFSNINASIEFGVK
                     DDIGVEFSPSFEFDSYKVDDVKYTNTGLGGRVIGKYYFSPNKGLDKWNIGPYIKYGYG
                     VGKGTNLSTKETVEVTRNRFAVGFYTGYKWVSRKNVVFELGFGLGRAFVNKYESDDQS
                     VDLDDFPILNLDATGKLALGYRFGGVSEGSGRGGKRR"
     gene            complement(1672..2304)
                     /locus_tag="PLE23_10870"
     CDS             complement(1672..2304)
                     /locus_tag="PLE23_10870"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HMX83809.1"
                     /translation="MSFTLPELPYAYNALESNIDAKTMEIHHTKHHNAYVTNLNAALA
                     GTEWEGKSLKELMSMVSKLPVAVRNNGGGHYNHSLFWTVMSPDGGGEPSDRMNIAKAI
                     DRDFGSFAAFKEKFSTAAKTVFGSGWAWLCVDKNDKLFVCATPNQDNPLMDVNDFDGT
                     PILGLDVWEHAYYLNYQNRRPDYVEAFFNVINWHEVTKRYNDANEGASDF"
     gene            2562..>3951
                     /gene="fumC"
                     /locus_tag="PLE23_10875"
     CDS             2562..>3951
                     /gene="fumC"
                     /locus_tag="PLE23_10875"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HMX83810.1"
                     /translation="MQFRTEKDTMGEVQVPVDAYYGAQTQRSIDNFKIARDINKMPRE
                     IIRAFAYLKKAAALTNRDAGVLPSDKCELISKVCDEILDGKLDDYFPLVVWQTGSGTQ
                     SNMNVNEVIAYRGHVLQGGSLLDETKFLHPNDDVNKSQSSNDTFPTAMHIAAYKMLIE
                     TTIPGIEKLRDTLAAKSTAFDNIVKIGRTHFMDATPLTLGQEFSGYVAQLNHGLRAIR
                     NTLGHLSELALGGTAVGTGINTPKGYSENVATHIAQLTGLPFITANNKFEALAAHDAI
                     VESHGALKTVAVSLMKIANDIRMLSSGPRSGIGEIHIPDNEPGSSIMPGKVNPTQCEA
                     LTMISAQVMGNDVAINIGGATGHFELNVFKPMMIYNFLHSARLIGEGCVSFNDKCATG
                     IEPIAQNIKQHVDNSLMLVTALNTKIGYDNAAKIAKKAHKEHLTLKQSAIELGLLTAE
                     QYDEWVRPENMVG"
BASE COUNT         1143 a         1031 c          708 g         1069 t
ORIGIN      
        1 gatactgacc ctattttttc ttcatattca cggcagatca tataatcatc tgcttcattt
       61 gccgaagtgg cagccttttg tgctgcaagg gcagctgctt ttatacgctc tgaaggcagg
      121 aatgctttgg cttcttcttc agcttcaacg gcacaagacc tcgctatctc ctgatggttg
      181 gcagctttga ggctgagtac ccatttgccg tctggcaata gccgtgtctg gtagtttttg
      241 tcttcagtca tgttggtttt tactgaagca ataccgtttt ctctggctgc ttccgtacca
      301 tagccttcac ttctgagaaa cacatttccg ttttccagaa aagagaaata aaatttgtca
      361 ttttcggaaa atctgttgaa gccgtgctcg tcgtattctc cgttcaagta tcgttcgcat
      421 gggagataat cgtccggtct gtggttttgg tcgctcatag tttgttattt tatggttaac
      481 agaatatctc tgaatggcaa atataatcat aagaccttaa tcttattaat tatttataca
      541 atatttagaa cgcaaaactc aaataaagtc acatatttta ttattccgac atttttcttg
      601 cagctatttt gtgaaaaata atgaaaaata tccgcaaaaa taccggaatg cacattacac
      661 cgaataagac ctcacgcaca ttcactccaa cgcttctcct tttttatcat acatctttta
      721 cccaatttcc acaaaaaaaa tgctacccga atcaacggac agcattttgt tctatgtaaa
      781 attttccagt gatttattta tctgcgcttg cctcctcttc ctgagccttc tgatacacca
      841 ccaaatctgt agcccaatgc gagtttgccg gtagcatcaa ggttaagaat cgggaaatca
      901 tccagatcta ccgactggtc atcgctttca tacttattga cgaatgctct gcccagacca
      961 aaacccagct caaacactac attctttcta gacacccatt tgtagccggt atagaatcca
     1021 accgcaaaac gatttctggt tacttcgacg gtttctttgg tactcagatt agttccttta
     1081 cccacaccat aaccgtattt gatgtacgga cctatattcc acttatcgag tcccttatta
     1141 ggagaaaaat agtattttcc tatcactctt ccacccagac cggtattggt gtatttcaca
     1201 tcatctacct tatatgagtc aaattcaaat gaaggcgaaa actctactcc gatgtcatcc
     1261 ttcacaccaa actcaatgga ggcattgatg ttgctgaaca acagcgatat tgggttaagc
     1321 gatacatcta cctgcgcatt tatactgtat gctcctgtga gcaataacac tcctaaaatg
     1381 attttcttca tactttttag ttttaaaaaa tgatttaata agttaaataa taattttttt
     1441 ctaatcatga tgcttctttg cattccctta atctacaatt catgtatata actcgattac
     1501 aaatactttt gctgcttgct atcagatttt tttcgaattt tgcacttttg acgatccggt
     1561 attattgcaa gtcacatccg cgagcaaaga ttaaattatt tcttttcaaa aaaaaatgcc
     1621 acctatatca acaggtagca tttcgacgaa cattttaaaa ttctttacca attaaaaatc
     1681 tgatgcacct tcattggcat cattgtatct tttggtgact tcgtgccagt tgatgacatt
     1741 gaagaatgct tctacataat ccggccttcg gttctggtaa ttgagataat atgcgtgctc
     1801 ccacacatcg agacctaata taggtgtacc gtcaaaatca ttgacatcca tcaagggatt
     1861 gtcctgatta ggagtggcac atacgaagag cttgtcattt ttgtcaacac aaagccatgc
     1921 ccatcctgag ccaaaaaccg tcttggcagc cgtactgaat ttttccttaa atgcagcaaa
     1981 agaaccaaag tcacggtcta ttgcctttgc tatattcatt ctgtctgacg gttctccgcc
     2041 gccgtcaggt gacatcactg tccagaacag cgagtggttg taatgacccc caccattatt
     2101 acgcacagca acaggcagct ttgataccat actcatgagt tccttcaaag atttgccttc
     2161 ccattccgtt cctgccaatg cggcattgag gttggtaaca taggcattgt gatgcttagt
     2221 atggtgaatc tccattgttt ttgcatcgat attcgactcc agtgcattgt aggcatacgg
     2281 gagttcaggt aatgtaaatg acatgatttt tacagtttag gttacgaaat aatatactgt
     2341 aaacatacaa attttaaact tgttgacaag tatccaagat aaattttctt aaaccaatag
     2401 cttccttttg ccctttattc atattcacta ccattcatac catcacaccc aacaccttac
     2461 tataccttcc ctccaatact atatttttac atttcattcc acatttaaac atccaaatac
     2521 cttagctttg tgtccgaatt taccaaaaaa actatatcat catgcaattc agaacagaaa
     2581 aagatacgat gggcgaagta caggtgcccg tcgatgccta ctatggcgca cagacacagc
     2641 gcagtatcga caattttaaa attgcccggg acattaacaa aatgcctcgc gaaatcatcc
     2701 gcgccttcgc ttacctcaaa aaagccgcag cactcaccaa ccgcgatgcc ggtgtactac
     2761 catcggataa atgcgaactt atatcaaaag tatgcgatga aatactcgat ggcaaactcg
     2821 atgactactt cccgctcgta gtctggcaga ccggcagtgg cacacaaagc aacatgaacg
     2881 tcaacgaagt catcgcctac cgcggacacg ttctacaagg cggaagcctc cttgacgaaa
     2941 ccaaattcct acacccgaac gacgacgtaa acaagtcgca gtccagcaac gacaccttcc
     3001 ctactgccat gcacatcgct gcctacaaaa tgctcatcga aacaaccata ccgggcatcg
     3061 aaaagctacg cgatacactg gccgccaaga gtacagcgtt cgacaacatt gtcaagattg
     3121 gccgtacaca cttcatggac gccacaccac tcaccctcgg gcaggaattc tcgggttatg
     3181 tggcacagct caaccacggc ctgcgcgcca tccgcaatac actcgggcac ctctctgagc
     3241 tcgccctcgg tggcacagca gtcggcacag gcatcaatac accaaaagga tactcagaga
     3301 acgtcgccac ccacatcgcc caactcaccg gcctgccttt catcacagcc aacaacaaat
     3361 tcgaagccct cgcagcacac gacgccatcg tagagagcca cggcgcactc aagaccgtag
     3421 cagtcagcct catgaagata gccaacgata tacgcatgct gtcatccggc cctcgaagcg
     3481 gcatcggcga gatccacata cccgacaacg aacccggctc atccatcatg cccggcaaag
     3541 tcaacccaac ccagtgcgag gcactcacca tgatcagcgc acaggtcatg ggcaacgacg
     3601 tagccatcaa tataggtggt gccaccggcc atttcgagct caacgtcttc aaacccatga
     3661 tgatatacaa cttcctgcac agcgcccgac tgataggcga aggttgtgtc agctttaacg
     3721 acaaatgcgc cacaggtata gagccgattg cacagaacat caaacaacac gtcgataaca
     3781 gcctcatgct cgtcaccgcc ctcaacacca aaatcggcta cgacaatgct gccaaaatag
     3841 ccaaaaaagc acacaaagaa cacctgacac tcaagcagtc cgccatcgaa ctcggcctac
     3901 tcacagccga acaatacgac gagtgggtac gccccgagaa catggtaggc a
//
LOCUS       DAOQQY010000265         3943 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11054_length_3943_cov_4.626800, whole genome shotgun sequence.
ACCESSION   DAOQQY010000265 DAOQQY010000000
VERSION     DAOQQY010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11054_length_3943_cov_4.626800"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..1290)
                     /gene="rfaD"
                     /locus_tag="PLE23_10880"
     CDS             complement(301..1290)
                     /gene="rfaD"
                     /locus_tag="PLE23_10880"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HMX83811.1"
                     /translation="MSPIRKSRIIVTGAAGFIGSCMVEKLNEMGHIYDIIVVDDFYKQ
                     YKDNNLRKKFIRDWIHRDIFLGLSENLAGIVDCVIHLGARTDTTEQNVEIFNKLNVEY
                     SKALWKFCVKHQIPFIYASSAATYGNGENGYDDDESKIPALKPLNPYGDSKQQFDVWV
                     LQQKETPPFWAGFKFFNVYGPNEYHKARMASVIFHSFNQIKANGKMKLFKSHRPDFED
                     GHQSRDFIYVKDLVDVLYYFFKHRKHSGIYNLGSGKARTFLDLTKAVFKNLDMKENIE
                     FIDTPADIRDTYQYFTEANMAKIRLAGYEKEFTSLEEGVADYVKNYLALGKYY"
     gene            complement(1290..>3943)
                     /gene="gcvP"
                     /locus_tag="PLE23_10885"
     CDS             complement(1290..>3943)
                     /gene="gcvP"
                     /locus_tag="PLE23_10885"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HMX83812.1"
                     /translation="KNFIGRGYYGTITPSVISRNIFQNPGWYTQYTPYQAEIAQGRLE
                     ALLNFQTMVSDLTGLPIANASLLDEGTAAAEAMHMFYAMKAKKNKEESPDTFLVDTGV
                     LEQTYDVLLTRAEPLDIKVKKVSWKEMELDGTVFGILLQYPDKNGSVDDYRALADKAK
                     AANISVTVAADLLALTHLMPPGEWGADAVVGNTQRLGVPMGYGGPHAAYFSTHEEYKR
                     NIPGRIIGVSIDVHGNRALRMALQTREQHIKREKATSNICTAQALLAIMAGMYGVYHG
                     PKGLKAIAARIHSLTVLLGKGIESLGLNIENTHFFDTISISTSAELQSKIREAALKAE
                     INFFYGTDSITIAVDETHAAKDIRAVLDVLESATGKKATFDTSADPGNKIPETLARTS
                     EFMTHEIFHRYHTESNMMRYIKSLENKDLSLMHSMISLGSCTMKLNAASELIPVSWDE
                     FANIHPFVPQDQVAGYNQIFKELEYYLCQATGFEACSLQPNSGAQGEYAGLLTIRAYH
                     QANGGAHRNIALIPASAHGTNPASAVMAGMDVVVIPCDDKGNIIVEELKRNAELHKDN
                     LSCLMVTYPSTHGVFETAIKEICDIIHTNGGLVYMDGANMNAQVGLTSPGHINADVCH
                     LNLHKTFAIPHGGGGPGMGPICVNKKLAPYLPSHVFVKTGGEKGIHAVSATPYGSASI
                     VLISYGYIRMLGERGLTLATKLAIMNANYIKAKLQNAYEVLYTGEKGNVAHELIVDLR
                     PFKAFVSAEDVAKRLMDYGFHAPTLSFPVAGTIMIEPTESESKSEIDRFCDALLEIKK
                     EIDEIATGKADAQNNVLANAPHTQNQICADVWDYPYSREKAAFPLPYLKSGKFWATIG
                     RVNNAFGDRNLICVCPPIEDYIKEENN"
BASE COUNT          999 a          840 c          849 g         1255 t
ORIGIN      
        1 accgcgattc ttaaaaggat atattcaggt gtttaaagaa taaatatttt tggccaattc
       61 gttaaacctg tttgctaaaa acttaaccct attttcaccg ccaaactgat cgacataacc
      121 atcggtcatc aaatagatta catctccttt ctcaagttga aatgaatttg ttgaaaatgt
      181 aattcggtac aaagttcact tatgatgaac acatttgcaa tagctttagt cgcataacag
      241 gccttaaata gtactaaata tttgttaaac tttcaattgt aaaatataaa gtgctgatta
      301 tcaatagtat ttgccaagag ctaaataatt tttcacataa tctgcaacac cttcttccag
      361 actagtaaat tccttttcat agcctgccaa gcggattttt gccatattgg cttccgtaaa
      421 gtactggtac gtgtcacgga tgtctgccgg tgtatctatg aattcaatgt tttctttcat
      481 gtctaggttc ttgaatactg cctttgtaag gtcaaggaat gttcttgcct ttcctgagcc
      541 cagattgtag atacctgaat gcttcctgtg tttgaaaaaa taatacaaca catctaccag
      601 gtcttttaca taaataaagt cgcgcgattg atgtccatct tcaaaatcag gcctgtgcga
      661 cttgaacaat ttcattttac catttgcttt tatctggtta aatgaatgga atatgacgct
      721 tgccattcga gctttgtggt actcatttgg tccatataca ttgaaaaatt taaatcctgc
      781 ccaaaaaggt ggagtttctt tctgttgtaa tacccaaaca tcaaactgct gctttgagtc
      841 gccgtatgga ttgagaggtt ttaaggcagg aatttttgat tcatcatcat cgtacccgtt
      901 ttcgccattg ccatacgtag cagcactact agcatagatg aacggaatct gatgttttac
      961 acaaaatttc cataaagctt ttgagtattc tacgttaagt ttgttgaata tttcgacatt
     1021 ttgctcagta gtatccgttc tggcgccaag atggatgaca caatcgacga tacctgccag
     1081 gttttcagat aaaccaagga atatgtcacg gtgtatccag tcacggatga attttttacg
     1141 aaggttgttg tccttatatt gtttgtaaaa atcatccaca actataatgt cgtagatatg
     1201 acccatctcg ttgagttttt ctaccataca gctaccaata aaaccggctg caccagtgac
     1261 gataatcctt gattttctga ttggagacat tagttgtttt cttcttttat ataatcttct
     1321 attggagggc aaacacatat taggttgcgg tctccgaatg cattattcac tctgcctatg
     1381 gtagcccaga attttccgga tttcagataa ggaagcggaa acgcagcttt ttctcttgaa
     1441 tatggataat cccacacatc agcacatatt tgattttggg tgtgtggtgc atttgccagt
     1501 acattatttt gggcatctgc ttttcctgta gctatttcgt cgatctcttt ctttatttcg
     1561 agcaaggcat cacagaaacg atctatctca gatttgcttt cgctctctgt tggctctatc
     1621 atgatggtgc cagctaccgg gaacgaaaga gtaggagcgt ggaatccgta atccattagg
     1681 cgcttggcta catcttctgc actcacgaac gctttgaatg gtcttagatc aactatgagt
     1741 tcgtgcgcta catttccttt ttcgccggta taaagcactt cgtatgcgtt ctgcagcttt
     1801 gccttaatat aattggcatt catgatagca agtttggtgg ctaatgtaag tccgcgttcg
     1861 ccgagcattc tgatataacc ataagaaatc aatacgatgc ttgcacttcc gtaaggggta
     1921 gcagatactg catggatgcc tttttcgcct ccggttttaa caaaaacatg cgaaggcagg
     1981 taaggagcga gtttcttgtt tacacatatc gggcccatac ccggaccacc gccaccgtgt
     2041 gggatggcaa atgttttatg tagattgagg tgacagacgt cagcatttat gtgtccgggg
     2101 ctggtaaggc ctacctgagc gttcatattg gcaccatcca tatatacaag gccaccgttc
     2161 gtgtggatga tgtcacaaat ctccttgatg gccgtttcga acacgccgtg cgtagaaggg
     2221 taggtgacca tcagacaaga aagattgtcc ttatgaagtt cggcattgcg cttcaactct
     2281 tctactatta tgttcccttt atcgtcacaa ggtatgacca caacatccat acctgccata
     2341 acggcacttg ccgggttggt accatgtgca ctggcaggta tcagagcaat gtttcggtgt
     2401 gcgccaccat tggcttgatg gtaagcgcgg atggtgagca gccctgcgta ttcgccctgt
     2461 gcacctgagt ttggctgcaa agaacaagct tcgaaaccag tagcctgaca aagataatat
     2521 tccagctctt tgaatatttg attataacct gctacctgat cttgaggcac aaatggatgt
     2581 atgttcgcaa actcgtccca acttacagga atgagttcgc tggcggcatt gagtttcatg
     2641 gtacaagagc caagcgatat cattgaatgc atcagagaaa gatctttgtt ctcgaggctt
     2701 ttaatatagc gcatcatgtt agactctgta tggtaccggt ggaatatttc atgcgtcatg
     2761 aattctgatg tgcgtgcaag tgtttcagga attttgttgc ccgggtcggc agatgtgtcg
     2821 aatgttgctt tcttgcctgt tgctgattcg agtacatcga gtactgctcg gatatctttt
     2881 gcagcatgcg tttcatcaac ggctatggtt atactgtctg ttccgtagaa gaagttgatt
     2941 tcagccttaa gggcagcctc gcggattttt gattgtagtt cagctgaagt actgatgctt
     3001 attgtgtcaa agaaatgagt gttttcgatg ttgagcccga gcgattctat tcctttacca
     3061 agtaatacag taagggaatg tattctggct gcaatagcct ttaggccttt cggcccgtga
     3121 tatacgccgt acatgccggc catgatggcc agtagtgctt gggcagtaca aatattggag
     3181 gtagcttttt cgcgtttgat gtgttgctct ctggtttgta atgccatacg tagcgcccta
     3241 ttgccatgca catctattga tacaccgatt atccttcccg gaatgtttct cttgtattct
     3301 tcgtgagtag aaaaataagc agcatgaggt ccgccatagc ccattggtac acctaggcgc
     3361 tgggtattgc ctaccactgc atctgcgccc cattcgcccg gaggcatcag gtgtgtgaga
     3421 gccagcaggt ctgctgcaac agttacactt atgtttgcag cttttgcttt atctgcaagc
     3481 gccctgtaat catctacaga gccgttttta tccggatatt gaagaaggat accgaatacc
     3541 gttccatcca gctccatttc tttccacgat acttttttta cttttatatc caatggttca
     3601 gctcgtgtga gtaatacatc gtatgtctgc tcgagtacac ctgtgtcaac aaggaaagtg
     3661 tccggcgatt cttctttgtt cttttttgcc ttcatagcgt aaaacatgtg catcgcttcg
     3721 gcagcagctg ttccttcatc aagtaacgat gcattggcta tcggcaaacc tgtaaggtcg
     3781 ctcaccatgg tctggaagtt gagtaaagcc tcaagtcgtc cctgtgctat ctcagcttgg
     3841 taaggtgtgt attgagtgta ccatcccggg ttctggaaaa tatttctgga aatcaccgaa
     3901 ggtgtaatgg tgccgtaata tccgcgacct atgaagtttt tga
//
LOCUS       DAOQQY010000266         3938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11071_length_3938_cov_5.630183, whole genome shotgun sequence.
ACCESSION   DAOQQY010000266 DAOQQY010000000
VERSION     DAOQQY010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11071_length_3938_cov_5.630183"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..328)
                     /locus_tag="PLE23_10890"
     CDS             complement(41..328)
                     /locus_tag="PLE23_10890"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HMX83813.1"
                     /translation="MDLNDLFKTVMSGGVADQIGNIAGVNNPEQAESAVGSIFSSILG
                     GIQKNVATPEGAESLNKALERDHDGSILDNLSGLLGGVFKPENANATNGSL"
     gene            537..1337
                     /locus_tag="PLE23_10895"
     CDS             537..1337
                     /locus_tag="PLE23_10895"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HMX83814.1"
                     /translation="MKNLLPFFLLLMMACQQKNDSEMAENQAFAAPEEKSGIAGKPPA
                     DMTIPEQAADRKLVKEGDISMEVKDLQTTGDELKKLTATYKAYLSNESQSAYDDRKQI
                     SYIVRIPVKDYEAFCENVISLGVNIERKNLNALDVTEEYVDTEARIATKKALEKRYLE
                     LLAKAKNVNEMLSIERELATVREEIESREARLKLLNNQSSYSTLNLTIYEIVNQSPGF
                     VRSAGQALAGGWFAMIKFALFLLRIWPFVLILAVVLYFGKKRFRPLKK"
     gene            1349..2101
                     /locus_tag="PLE23_10900"
     CDS             1349..2101
                     /locus_tag="PLE23_10900"
                     /inference="COORDINATES: protein motif:HMM:NF018473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HMX83815.1"
                     /translation="MKTILWIMLTAIFSVDGCTQNKKPQETTQDGFVLLELFTSQGCS
                     SCPPADENLSKIINAAMNNGKNIYALAFHVDYWNRLGWKDPFSSEQYSARQAMYVNKM
                     KLRSAYTPQMVVNGEREFVGSDAGAAEEAIQHFLNTKYKHSIILGSAATTDGIIESFP
                     YHIEGELSGTQLYAAIVESDKITEVKKGENKDKTLRNDNVVVWLGSFDPKPSGNVLLP
                     KEFTKGKPNTDVVIFLQDTTSGKVLAAQSIRL"
     gene            2167..3507
                     /locus_tag="PLE23_10905"
     CDS             2167..3507
                     /locus_tag="PLE23_10905"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HMX83816.1"
                     /translation="MRTTTILKLAICACALLILQSCAKDKIVNSFDKTASFDSKIAHE
                     WFALQSDLIKNTSGFSGPVAARSFGYAGITLYQAVYQGMPGYNSLEGKIEGLKPNSIT
                     SVSDRTKYHWAAVANAAMAEFFRLQFKTTSVENLDRINELELYYKDIYTKLTNPDIID
                     ASVQYGRTLALEMNQYAISDGQSEAYSTNYPAYIPASGAGKWVPAGKEKTPLQPYWGS
                     TRSFLTGNIVAGKTPEPPVYSEEKSSQLYKEAYELFTIRENITTYQSDIAKFWDDATG
                     KSSGTAGHIFFIAGTVLESQRSNLAKAAEVYCRLGIALHDACIAGWKTKFQYNTLSPV
                     TFIRKQIDGDFTPVLPPPPYPEYSSAHSVLAGAGFTVLSEYLGSSYSFADYSNYKRAD
                     INGSPKNFSSFMEAAAEAGASRLYGGVNFRSSVDKGLEQGIRIGGNTSKFSLKQ"
     gene            3730..>3938
                     /locus_tag="PLE23_10910"
     CDS             3730..>3938
                     /locus_tag="PLE23_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX83817.1"
                     /translation="MKSGKHPKALPYLFFTEMWERFGYYLMIGIFVLYLTDIDKGGFG
                     MSRATAADIYGTFIAFVYLTPFIGG"
BASE COUNT         1264 a          834 c          771 g         1069 t
ORIGIN      
        1 atgtttttat atttttggta aaatttttga aatatttttt tcacagactg ccgtttgtag
       61 catttgcgtt ctcaggcttg aatacaccac ccaataatcc tgacagatta tcgagaatac
      121 tgccatcatg atctctttca agtgctttgt tcaaagattc tgctccttca ggagtagcaa
      181 cgtttttttg aattccgccc aatattgatg aaaaaatact gcctactgct gactctgcct
      241 gttcaggatt attcactcct gcgatattac caatctgatc ggctacaccg cctgacatta
      301 ctgttttaaa aaggtcgtta agatccatgt tctaaatatt tatttaatgg ttataaaatt
      361 acaaaggtaa caaaagtctg tctcttttgg ttccgtcttt ttgaaagtga atattgtatt
      421 taatataaat ttaaatttta ctcgaagaca ttgacacttt attaacaata caggtacagt
      481 agcaaaccaa ctgcttgtct tatcttgtca tcatgtatgt ataaataact cctacaatga
      541 aaaatttatt gccttttttc ctcttattga tgatggcttg tcaacaaaaa aacgattctg
      601 agatggctga aaatcaagcc ttcgctgcac ctgaagaaaa atcaggaatt gctggcaaac
      661 cacctgccga tatgaccata ccagaacaag ccgctgaccg taaactcgtg aaggaaggcg
      721 atatatctat ggaagtaaag gacctgcaga ctaccggtga cgagctaaaa aaacttactg
      781 caacatacaa ggcttacctc tcaaacgaat cgcaatcggc ttatgatgac cgaaagcaaa
      841 taagctatat agtacgtatt cctgttaagg attatgaggc attttgtgaa aatgtaatca
      901 gcctcggtgt caatattgag cgcaaaaacc tgaacgccct ggatgtaaca gaagaatatg
      961 tggacacaga agcccgcatt gctaccaaaa aagcactcga aaaaagatac ctcgaactgc
     1021 tggcaaaagc aaaaaacgtg aatgaaatgc tcagtataga acgcgaactg gctactgtac
     1081 gcgaagaaat tgaatcgcga gaagccaggc tcaaattact caataaccag tcttcttatt
     1141 ctacgctcaa ccttactatc tacgaaatcg tcaatcagtc gcccggcttt gttcgttcgg
     1201 caggacaagc tttggccggc ggttggtttg caatgataaa atttgccctt ttcctgctta
     1261 ggatatggcc atttgtactt attttggcag tcgttttgta ttttggcaaa aaacggttca
     1321 gaccacttaa gaaataatca ttcgaccaat gaaaacaata ctctggataa tgctgacagc
     1381 catcttttcg gttgacggat gcacgcagaa taaaaaacca caagaaacaa cccaagacgg
     1441 atttgtactg ctcgaactat ttacatcaca aggttgttct tcgtgcccgc ctgctgacga
     1501 aaatctttcc aaaatcataa atgctgcaat gaataatggt aaaaacatct atgccctagc
     1561 atttcacgtc gattattgga atcgtcttgg ctggaaagac ccgttcagtt ctgaacaata
     1621 ctctgcacga caagctatgt atgtcaacaa aatgaagctg cgttctgcat acacaccgca
     1681 gatggtggtc aatggcgaac gcgaattcgt gggttcagac gcaggagctg ccgaagaagc
     1741 cattcaacat tttcttaaca cgaaatataa acactctatc atacttggct cagcagccac
     1801 cacagacggt atcattgaat cttttcctta ccacattgaa ggtgagctca gtggcaccca
     1861 actctatgca gctattgtag agagcgacaa aattactgaa gtgaagaaag gcgaaaacaa
     1921 agacaaaacc ctcagaaacg acaacgtagt agtatggtta ggctcatttg acccaaaacc
     1981 atccggcaat gtcctcctgc ctaaagaatt tacaaagggc aaaccgaata ccgatgttgt
     2041 catttttctt caagacacca catcaggcaa agtattggct gcacagagca tcagattgta
     2101 atatttgatt tttttggtat agattttgtc ctataaggat aacgtcctat acatatcaaa
     2161 tttacaatga gaacaacaac aatcctaaaa ctggcaatct gtgcctgtgc acttctgatt
     2221 ctacaaagtt gcgccaaaga caaaatagtc aattcctttg ataaaacagc ttcctttgac
     2281 agtaaaatag cacacgaatg gtttgcgttg caatctgacc tcatcaagaa tacaagtgga
     2341 ttctcggggc cggtggcagc tcgttcattc ggctatgccg gaataacact ctaccaggca
     2401 gtgtaccagg gtatgcccgg gtataattcg ctggaaggga agattgaagg attaaaacca
     2461 aacagtatca cttctgtatc tgaccgtacc aaataccatt gggcagcggt ggccaatgct
     2521 gcaatggcgg agtttttcag actccagttc aagacaacat cagttgagaa ccttgaccga
     2581 attaatgaac ttgaactgta ttataaagac atttatacaa aattaaccaa ccctgacatt
     2641 attgatgcaa gtgtacaata cggcagaaca ttggcacttg agatgaacca gtatgccata
     2701 tccgatggac agagtgaagc ctacagcacc aattatcctg cttatatacc ggcaagtggt
     2761 gcaggcaaat gggtgccggc tggcaaggaa aaaacacctt tgcaaccata ctggggaagt
     2821 acgcgttctt ttctcaccgg caatattgtt gcaggcaaga cccctgaacc acctgtatat
     2881 tcagaagaaa aatcaagtca attgtataaa gaagcctacg aactcttcac aattcgtgaa
     2941 aatataacca cctaccaatc ggatattgca aaattctggg acgatgctac cggcaagagt
     3001 agcggtacag ccgggcacat cttttttata gcaggcacag tacttgaatc acaacgcagc
     3061 aatcttgcca aagccgcaga ggtatattgc agactcggta ttgcactgca tgatgcctgt
     3121 attgctggtt ggaaaaccaa atttcagtac aatacactga gcccggttac atttattaga
     3181 aagcagatag atggagattt tacacctgta ctaccaccgc cgccttaccc cgaatactca
     3241 agtgcacatt ctgtactggc aggtgcaggg tttacagttt tgtcagaata tcttggtagt
     3301 agttactcat ttgctgacta tagtaactat aagcgagctg atataaatgg cagcccaaag
     3361 aatttcagta gttttatgga agcagcagct gaggcaggag catcaagact gtatggtgga
     3421 gttaattttc gctcgtctgt tgacaaaggg cttgaacagg gtattagaat aggaggaaat
     3481 acaagtaaat tttcattaaa acaataaaaa acaccaaaca ccaaccaaat acaggaaatc
     3541 aagtgccggt atcgggagta atcaagggat aaccaaaaaa aacattgcta gaaatacatt
     3601 cccggcaatg atatgcagta tctgcataca tgccacactc atgaaccgga ctgattttca
     3661 aattatttta cctgattcat gtatatttta taaaaaaacc ttttctttga aaattaatca
     3721 aggcatttta tgaaatcagg aaaacatcca aaggcattac cgtatttatt ctttaccgaa
     3781 atgtgggaac gcttcggcta ctacctcatg ataggtatat tcgtcttgta ccttacagat
     3841 attgacaaag gaggcttcgg gatgagccgt gccacagcag ccgacatata cgggaccttc
     3901 attgcattcg tttatttaac accattcatt ggtggttt
//
LOCUS       DAOQQY010000267         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11078_length_3937_cov_5.469603, whole genome shotgun sequence.
ACCESSION   DAOQQY010000267 DAOQQY010000000
VERSION     DAOQQY010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11078_length_3937_cov_5.469603"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(278..742)
                     /locus_tag="PLE23_10915"
     CDS             complement(278..742)
                     /locus_tag="PLE23_10915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMX83818.1"
                     /translation="MKKLLYTLGLLILVTACSKEDMLTPEQQLAKDTEIIEEYIKANQ
                     LTAQKTASGLYYVITKEGTGDHPTINSKIKIKYKGYLVNKVVFDKTVGDEVLNDQLSK
                     FIAGWQEGIPLFKKGGTGTLLIPSYLGYGSQSIGPIPKNSVLIFDIELVDFI"
     gene            complement(851..>3937)
                     /locus_tag="PLE23_10920"
     CDS             complement(851..>3937)
                     /locus_tag="PLE23_10920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83819.1"
                     /translation="ITQPADIIAAGILTDSISCKGLSDGKASATVQGGTMPYSFLWSN
                     GSTDSIATNVPLGPAQVTITDQNGCQKTTSVTMLEPEAISATTSTVNPKCFNGSDGTI
                     SVTPNGGTAPYTYLWDNNAGNQITQIATALAEGTYHVTITDKNGCTQTSQASISAPTA
                     VTLSVTAKNATCFGVSDGSATAIADGGTTPYTYIWDDPLAQTGFQATNLAAGTYHVTV
                     TDGAGCIATAEVVISSPPELNATVTVTPTLCFGSSDGTASVNTFGGKAPYTYIWSDNG
                     AKVATRNDLAAGTYSVTITDANGCTAVKDNIVISSPNTADIQLTGTDVLCFGGKSGTA
                     TATANGGNGGWTYLWNDPLNQDLPTASFLTAGAYSVTATDISGCTITSSITIGEPAEL
                     TAAPTTKDVTCFGGSDGSISINASGGTGNYSYTWNPGNVSGPSPTGLAAGNYTVTVSD
                     QNNCQKIFDITINQPEAIDIEFQVGNVSCSGVFNGVATAIVNGGTAPYTYKWSDPQGQ
                     TTQTVTGLAAGTYTVTVTDAGGCSQTAIFDISQPAAIIVDLIAIDVTCYGGSNGSVGS
                     TAYGGTTPYTFIWSNGSNINVANNLTAGIYSLTVTDAMGCSVTKEIEVKQPEDMVILP
                     TITGINCPGDKTGSITVSVSGGLQPYSGKWSNGDTGFTASNLGAGTYTLQLTDSKGCL
                     KTASYTISPVDTLDLNFIVTNVSCFGFADGIIEAQPDGGTPGYKYSWSTGASSQIVTG
                     LAPGTYSLTITDSKSCTYSESIPVVEPDAALTAIVVGNDIDCFGEKTGEVIFTAQGGT
                     PPLYYSIDGNNFSPNSIAIGLGSGSYSCKVKDKNGCTVDAGIAVVTEPPPFSVTLGPD
                     IHIKLRRDTQLNAVVTNGIGVLNYKWIVYDTTLLSCQNCPNPKLPALTDPVLFKLVVT
                     DENGCTAYDDIVVYVDKTRYVFVPTAFTPNADNVNDVLLTHGEEDAFVEEFRIFDSWG
                     EMVYEAKEFYLGDKNVFWDGKFRNKDMNPGTFVWYMRIKFIDGDVELLKGSFDLLR"
BASE COUNT         1057 a          843 c          830 g         1207 t
ORIGIN      
        1 tttggattat attcaagttt ttaaaattaa gaatttattt gcaaataacg cataaaatac
       61 aaaatataat ttcgaataat acaaactgct ttgtagtatt tataaaatgt ctattactca
      121 tcctgcatgt tgctgaatgt tcaacagtca aaacaatgtt tagatgcgaa taagaccgta
      181 gtattgtttc gcactcatat cataatgata caacgcagtt acagctatta tttgttccgg
      241 atgtggataa gaaattgtct gttatttgta aaatttatca aatgaagtca accaattcaa
      301 tatcgaagat tagaacggag tttttcggga taggacctat actttgtgaa ccataaccca
      361 agtacgacgg aatcaaaagt gtgcctgtgc ctcccttctt gaaaagggga atgccttcct
      421 gccagcctgc aataaatttt gacaattgat cgttcaggac ttcatctcct acggttttgt
      481 caaaaacgac tttattgacc agatagcctt tgtatttaat ctttattttt gagttaatgg
      541 tagggtggtc acctgttcct tctttagtga tgacataata aagcccggag gctgtttttt
      601 gtgcagtgag ctgattagct ttaatatatt cttcgatgat ctcggtatct ttggcaagtt
      661 gttgttcggg tgtgagcata tcttctttgc tgcatgccgt taccaagatc aacaaaccga
      721 gtgtgtacaa caattttttc atatcagatt ttttacaaaa ataatcagct ttttcaataa
      781 ttgactatat gatgaaaaca aaagggaatc cgtagcgaat tcccttctat tttagttata
      841 aatttatcac ttaccgaagc aaatcaaatg atccttttaa tagctcgaca tctccgtcta
      901 taaacttgat gcgcatatac cagacaaatg tacccgggtt catatcctta tttcggaatt
      961 ttccatccca gaatacattt ttatccccga ggtaaaattc ttttgcttcg tacaccattt
     1021 cgccccaaga atcgaatatc ctgaattcct caacgaatgc atcttcttct ccatgtgtga
     1081 gcagcacatc attgacgttg tccgcgttag gtgtaaatgc cgttggtacg aaaacatacc
     1141 gtgttttgtc aacatatact acaatatcgt cataagccgt acaaccattt tcgtctgtta
     1201 ccacaagctt aaataatacc gggtctgtca gtgcaggtag ttttggattc ggacaattct
     1261 gacaactaag cagggtagta tcatacacta tccatttata attcaacact cctataccat
     1321 ttgtgactac tgcattgagt tgtgtatcgc ggcgcaactt gatatggatg tccggcccca
     1381 aagtaactga gaatggaggt ggttctgtga caacagcaat tccggcatcg actgtacatc
     1441 cgtttttgtc tttcacctta caagaatatg agcctgaacc tagcccgatg gcaatactgt
     1501 ttggtgaaaa attattgcca tcaatactgt aatagagtgg tggcgtacca ccctgagctg
     1561 tgaatattac ctcaccggtc ttctctccga agcaatcaat atcgttacct acaacaatgg
     1621 ctgtaagagc ggcatcaggc tctaccacag gtattgattc tgaataagta cacgatttgc
     1681 tgtcggtaat ggtgagagaa taagtgccgg gtgccagtcc ggtcactatc tgagagcttg
     1741 ctccggtact ccagctatat ttatatcccg gtgtgccacc gtcaggttgt gcttcaatga
     1801 tgccatcagc aaagccaaaa cagctgacgt tagtgactat gaaattaaga tcaagcgtat
     1861 caaccgggct gatggtgtaa gatgcagtct ttaggcatcc ttttgaatct gtgagttgaa
     1921 gtgtataggt accagcacca aggttcgatg ctgtaaatcc tgtgtcacca ttactccatt
     1981 tgcccgaata aggttgtaaa ccacctgaaa cacttacagt aatgctacca gttttgtcgc
     2041 cagggcaatt aatacctgtg atggtaggca gaattaccat atcctccggt tgtttgactt
     2101 caatctcttt ggtcaccgag catcccatag catctgttac ggtaagtgaa tagattcctg
     2161 cggttaggtt attggctaca ttaatgtttg acccgttgct ccagataaaa gtataaggtg
     2221 tagtacctcc ataagcagta gatccgacag agccattgct tccgccataa catgtaacat
     2281 caatagctat gaggtcaaca ataattgcgg caggttgaga aatatcaaat atcgcggtct
     2341 gcgagcaccc accggcgtct gtaactgtta cagtataggt gccggctgcc aaaccggtta
     2401 ctgtttgggt ggtttgcccc tgcggatcac tccatttgta tgtatatggg gctgtacctc
     2461 cattaacgat tgcggtagcc acaccgttga atacaccact acaacttaca tttcctactt
     2521 gaaattcgat atcgattgct tctggttggt taatggttat gtcaaatatc ttttggcaat
     2581 tgttttggtc agaaactgta actgtatagt ttccggcagc gagccctgtt ggggatggtc
     2641 cacttacgtt accagggttc catgtgtaag aataatttcc tgttccgcct gaagcgttta
     2701 ttgaaatact tccgtcacta cctccaaaac aagttacatc tttggtagta ggtgcagctg
     2761 ttaattcagc cggttcaccg atagtgatgc tactcgtgat ggtacagccg gatatgtcag
     2821 tagctgtaac tgaataggcg ccggcagtca ggaacgatgc ggtaggtaaa tcctggttga
     2881 gcggatcatt ccagagataa gtccatcctc cattaccacc atttgccgtt gcagtagcag
     2941 tacctgattt tccaccaaaa cacaatacgt cagtacctgt aagttggata tcggcagtat
     3001 tgggtgaaga tataacgata ttgtccttga cagctgtaca tccattggcg tcagtgatgg
     3061 taaccgaata agtgcctgca gcaagatcat ttcttgttgc tacttttgcg ccattatcgc
     3121 tccaaatata agtataaggg gcctttcctc caaaggtgtt gacagatgca gtaccatcgc
     3181 tggagccaaa acataaggtc ggcgtaactg taaccgtcgc gttcagctca ggaggcgaag
     3241 agatgactac ctccgcagtt gcaatacatc ctgcaccatc ggttacagta acatgataag
     3301 tgcctgcggc cagattggtt gcctggaagc ctgtttgagc caatggatca tcccaaatat
     3361 atgtataagg tgttgtacct ccgtctgcaa tagccgtagc actaccatct gaaaccccga
     3421 aacatgttgc atttttggca gtgacgctta gtgttactgc ggttggcgcc gatatgcttg
     3481 cctggcttgt ttgtgtgcaa ccgtttttgt ctgtgatggt aacatggtaa gtaccttctg
     3541 ccaatgcagt agctatttgt gttatctgat tgcctgcatt attatcccaa agataagtgt
     3601 atggtgctgt accaccattt ggggtcacgc taatagtgcc atctgagccg ttaaaacatt
     3661 taggattgac agtactggta gttgcactaa ttgcctccgg ctccaacatt gtaactgaag
     3721 ttgttttttg acaaccattc tggtcggtaa tggttacctg tgcgggtccg agtggtacat
     3781 tggtagcaat agaatccgtt gatccattgc tccacaagaa tgaatatggc attgtacctc
     3841 cctgaacagt tgcacttgct ttaccgtccg acaagccttt acaactgatg ctgtccgtca
     3901 atattcctgc tgctattatg tctgcgggtt gggttat
//
LOCUS       DAOQQY010000268         3926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11127_length_3926_cov_6.096874, whole genome shotgun sequence.
ACCESSION   DAOQQY010000268 DAOQQY010000000
VERSION     DAOQQY010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_3926_cov_6.096874"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="PLE23_10925"
     CDS             complement(<1..262)
                     /locus_tag="PLE23_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83820.1"
                     /translation="MNSIINLGRYLFAIPMAIFGAFHFMNAAAMAEMVPVPGGVVWVY
                     VAGISLIMAAVAIFLGKLDKLAAVLLAVELLLFAILIHLPNAM"
     gene            complement(393..1076)
                     /locus_tag="PLE23_10930"
     CDS             complement(393..1076)
                     /locus_tag="PLE23_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83821.1"
                     /translation="MEKVIIPAWKKWLSWLFPIRLEESSSDFNDLLVVSLSKGRLQLL
                     TENAIYSFDDLYDNFDFAFSKLRLDNINETLVLGLGLGSIPYLLEKKYGIHTNITAVE
                     IDEEIIYLAGKYSLHRLKNPTDCICTRAEIFLDVTEEKFDLICMDVFVDEQIPEVFLS
                     TNFLETIREKITPGGIFLFNHLATSTEDKEKAQLYFDEIFLSVFPAGKILKAPHNYIL
                     LQDKTREKA"
     gene            complement(1064..2251)
                     /locus_tag="PLE23_10935"
     CDS             complement(1064..2251)
                     /locus_tag="PLE23_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmU family protein"
                     /protein_id="HMX83822.1"
                     /translation="MLNIILTDTTAWNDLLPLSYTRPIALLRTGILTIAEKWQKISGA
                     DVSYLTQSHLKNKFPACLADDSIVVDGSILPNKHLLVELQNLPTDTSLIHNNQWIASR
                     LSRSLTEEFPSCTGHLAPKESVAPVLRIETLADIIRLHGTQMKLDFDLLTLGRTSEKL
                     SETNRIIGPPNNIFVEKGAKVECATFNTEAGPIYIGEDAEVMEGSIIRGPFGMGAHAV
                     VKMGAKIYPNVSIGPHCKLGGEAGNSILYAYSNKGHDGYLGDSYIGEWCNLGADTNTS
                     NLKNNYAEVKLWNYPKGRFLPTGLQFCGLIMGDHSKAGINTMFNTGTVVGVSCNIFGD
                     GYPRNFIPSFSWGGAGGLSTFVLDKATEACNAMMSRRGLQLTEADKQIFGHIYECDKA
                     WRK"
     gene            complement(2432..2683)
                     /locus_tag="PLE23_10940"
     CDS             complement(2432..2683)
                     /locus_tag="PLE23_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HMX83823.1"
                     /translation="MKKDIHPSNYRYVVFKDVSTDDAFLTRSCMNSRETTTWTDGKEY
                     PLIKLEITAHSHPFFTGKMKFVDTAGRIDKFNKKYAKFM"
     gene            3101..>3926
                     /locus_tag="PLE23_10945"
     CDS             3101..>3926
                     /locus_tag="PLE23_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83824.1"
                     /translation="MKKNVISRLLKLSVFMIPVTCAWYGKAQILIDPYYISPATKNGY
                     KLIFRDEFNGNNLDLQNWDISTSNKPDDVPCKWYLYDFMLGQVTVKDGMCLLKTEKAQ
                     QDVNTNCLVYPDCERGVSGEIKNTSWRDPYCIEPDFPGVCFQNYDFPVGSYIEIRAKT
                     TDADCNAGSAFWLTGGNQEIDVFETSGDEDDFTSGFFSSRIINGKEYTEYERYRNWWD
                     KLWGLEGKEPKIISLGFTMNRQIEVTKTLLVWEEVNQKWTEQTFTQLKEGPHIKPSD"
BASE COUNT         1066 a          795 c          873 g         1192 t
ORIGIN      
        1 ccatagcatt cggcaaatgg atgagtattg caaataacag caattctaca gccagcaata
       61 ctgctgccag cttgtcgagt ttgccaagaa aaatggcaac tgccgccatg atgagcgaaa
      121 tacctgccac atatacccag actacacctc ccggtaccgg caccatttca gccattgcag
      181 cggcattcat aaagtgaaat gcaccaaata ttgccatcgg tatcgcaaac aaatacctgc
      241 ccagattaat aatcgaattc attatcttaa tcttttattg gttcgaaaaa cgacttgtca
      301 tggctaagtt aagcagtttg ttgtaaataa tgtcgttatc ttataaaaaa atcgctcaat
      361 actgcccaaa cgagagattt ccggtactca gctcaggctt tttctcttgt tttatcctgc
      421 agcaagatat aattatgggg cgcttttaat atctttcctg ccgggaatac actcaggaat
      481 atttcatcaa aataaagttg tgccttttct ttgtcttcag tgcttgtagc cagatggtta
      541 aacagaaata tgcccccggg tgtgatcttc tcacggatgg tttcaaggaa atttgtgctg
      601 agaaacactt caggaatctg ctcatctacg aatacatcca tacagatcag gtcaaatttt
      661 tcttcggtta catccaggaa gatttcggca cgggtgcaga tgcagtctgt cgggtttttc
      721 aggcggtgca ggctgtattt tcctgccaaa taaatgattt cttcgtctat ctctactgcc
      781 gtaatgttgg tgtggatacc atattttttc tccagcagat aaggaatact tcccaggccg
      841 agccctagta cgagtgtttc attgatgtta tccaacctaa gcttgctgaa ggcaaaatca
      901 aaattatcgt acaggtcatc gaatgaataa atagcattct cggtcagaag ctgtagtctg
      961 cctttggaga gacttaccac caacaggtca ttaaaatcag acgaactctc ttcgagcctg
     1021 atagggaata accacgagag ccattttttc catgcaggta tgattacttt ctccacgcct
     1081 tatcgcattc atagatgtgt ccaaatattt gtttgtctgc ttctgtcagc tgcagtcctc
     1141 gacgcgacat catggcattg caggcttctg ttgctttatc caatacaaag gtgctaagtc
     1201 cgcctgcgcc accccaggag aatgacggga tgaaattgcg cggatagcca tcaccgaata
     1261 tattgcatga cacgcctaca accgtacctg tattgaacat cgtattgatg cctgccttcg
     1321 aatgatcgcc catgatgagt ccacagaact gaaggccggt gggtagaaaa cgccctttcg
     1381 ggtagttcca gagttttact tcggcgtagt tgttcttcag gttggaggtg ttggtgtctg
     1441 caccgagatt gcaccattcg ccgatgtaag agtcgccgag gtagccgtca tggcctttgt
     1501 tggaataagc atataggatg ctgttacctg cttctcctcc gagtttgcaa tgcggcccga
     1561 tgctcacgtt cggataaatc ttggcaccca tttttacgac cgcatgtgct cccatgccaa
     1621 acggccctcg tatgatgctg ccttccatca cttccgcatc ttcaccgatg tagatcggtc
     1681 cggcttcggt attgaatgtc gcacattcta cttttgcgcc cttctcgacg aaaatattat
     1741 tcggtgggcc tattatccgg tttgtttcag acagtttttc tgatgtccgc cccaaggtga
     1801 gcagatcaaa atcgagcttc atctgtgtac cgtgcagcct tatgatatcg gctaatgttt
     1861 cgattctcaa taccggtgct acagattctt ttggcgctaa atgcccggtg caggaaggaa
     1921 attcctctgt caaactccgg ctgagccttg atgcaatcca ctggttgttg tggatgaggc
     1981 ttgtgtcggt aggaaggttt tggagttcga caagcagatg cttatttggc aaaattgatc
     2041 catccactac gatactgtca tctgccaggc aagccggaaa tttgttctta agatgtgatt
     2101 gtgtcaaata ggaaacatct gcacctgata ttttttgcca tttttcggca atggtgagta
     2161 tgcctgtacg caagagagct atcgggcgtg tgtagctgag cggaagcagg tcattccagg
     2221 cagttgtatc ggttaatata atattaagca tgagtggtct ttgatgacca aagttagaat
     2281 gaatagtttg tatagtaaaa tatttggagt gaatgttgtg tggtggaatg tttttgtctt
     2341 tcttatggtg aaaatttgtc tgactaaaag gcaataaaaa agtcctgaag caaatcgcca
     2401 caggactttt atgtatattc tcaccaggag attacataaa tttagcgtat ttcttgttga
     2461 atttatcaat tcgacctgcg gtgtcaacga acttcatctt accggtaaag aacgggtgag
     2521 agtgagcagt aatctcaagc ttgatgagtg ggtattcctt gccgtctgtc caagtggtgg
     2581 tttctcttga gttcatacat gaacgagtga ggaacgcatc gtctgttgac acatctttga
     2641 atactacgta tctgtaatta gatggatgga tatctttttt catattattt tactttaaga
     2701 gtgtgcaaag ataaaaacta ttcttgataa tgtaaagaat tttttgtttt atttttctga
     2761 aaatcagcgg atgatgctca catcaccttt tagtaaaggt tccttgccgt cggttagttc
     2821 tattagcagg taataaacat acacaacttc ctggactttt tgtcctctgt gttttccgtc
     2881 ccatcttgcc tcgggttcgt tggtttggag aatgacgtct gttcctgtaa agattactag
     2941 atttgttcag tctaatttaa aaaaatattt agtttgtgaa caaatctatt ttttattcag
     3001 tgcaaatatt tatatttgca gaagtgttgt gtgtgtggga acatttttta gatttcaaaa
     3061 ctggcgcaat taattttttt aacaatttaa aaataacaac atgaaaaaga acgtgatcag
     3121 ccggttatta aagctttctg ttttcatgat tccggtgaca tgtgcatggt atggcaaggc
     3181 acagattctg attgatccat attatattag tcctgcgaca aaaaatggct ataagctgat
     3241 tttccgggac gaattcaacg gcaacaatct tgatttgcaa aactgggata tcagcaccag
     3301 caacaagcct gatgatgtcc cttgtaaatg gtacttatat gattttatgc tcgggcaggt
     3361 gactgttaag gatggtatgt gtctcctgaa aacagaaaaa gctcaacagg atgtcaatac
     3421 aaattgtctt gtttatccgg attgtgaaag aggcgtatcc ggggaaatca aaaacacgtc
     3481 gtggcgtgac ccctattgta ttgaacctga ttttccgggt gtatgctttc agaactatga
     3541 ttttccggtc ggttcgtata tagagattag ggcaaaaaca acggatgcgg actgtaatgc
     3601 tggcagcgcc ttttggctaa ccggcggtaa ccaggaaata gatgtctttg aaacaagcgg
     3661 ggatgaagat gattttacat cgggattctt ttcctccaga attataaacg gtaaggaata
     3721 tacggaatac gaacgctata gaaactggtg ggataagctc tggggcctgg agggcaaaga
     3781 gcctaaaatc atcagcctcg gctttaccat gaacaggcag attgaagtga ctaaaacctt
     3841 attagtttgg gaagaggtaa atcaaaaatg gactgaacaa actttcactc agctgaaaga
     3901 gggcccgcac atcaagccga gtgata
//
LOCUS       DAOQQY010000269         3921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11142_length_3921_cov_6.041904, whole genome shotgun sequence.
ACCESSION   DAOQQY010000269 DAOQQY010000000
VERSION     DAOQQY010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11142_length_3921_cov_6.041904"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..3515)
                     /locus_tag="PLE23_10950"
     CDS             complement(153..3515)
                     /locus_tag="PLE23_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HMX83825.1"
                     /translation="MSRKIFTVVAITLGMIACKNNSQPEENTVPSGPQRFELLSPEKT
                     GVTFSNHLKEDYVYNILNYEYLYNGGGVTVGDINNDGLPDIYFTATFGTNKLYLNKGN
                     MKFEDITDRAGVAANAGFKTGVTMADVNNDGYLDIYVCRTSREDDGKKDNYLYINNRD
                     LTFTNQAQEYNLAGNANTNHAVFFDYDLDGDLDMFMMNHRVGFDQATKTRLRQSDDGK
                     ITRETFAQTPFESSRLYRNDNGKYTDISASIGLNTSAFSLSATAADINMDNYPDIFVA
                     NDYIEPDFVFINNQKGGFTDKFSQILRHSSQNSMGSDVADFNNDGLPDIMTLDMISED
                     QVRYKQLMNIMQYERYNLLVQYGYGHQASRNMLQLNQGNDFFSEIGQMAGISNTDWSW
                     GCVFADFDNDTNKDIFITNGYRHDVTDNDYMVYTRDSIQKTGGVTPRRFPDVNEFLNI
                     IPEHKLPNYMFRNKGDLTFENMTNAWKTGEPSFSNGVAYADLDGDGDLDLVVNNILDP
                     AFIFENKTSQLADHTYLQIKLSGPDKNKFGLGTKAWIYNKGQMQYQEMYTNHGFMSSS
                     EPILHFGTGNEKSIERLEIRWPDGKTQVLTNVNTNQKLNLKYAEAGNQALTVLPKPAP
                     LFEDITKASKLDFVHSENEYIDFNYERLIPHYYSNLGPYIAHGDVNGDKLEDIYIGGA
                     TGNQEKQGEPGALYIQLPGGKFKRTIQPDFDKDRNCEDTDSKFLDVDSDGDLDLVVGS
                     GGNENPDGTDYYQLRIYTNDGKGNFKRNTNAIQDLKQNVGRFLVFDIDGDGDQDLLIG
                     GRVVPGAFPTIPKSFIYKNDKGVLRDATAEFCPEFSQMGMVSDIKAADLDGDKIPEIV
                     VCGEWMPITVFKKQGKVYKNAITSFGLEKTNGWWNSIVIADFDKDGDLDIVAGNLGKN
                     SRHRASEKSPIVMYAKDFDNNGSIDPVMCFTRNGNIFPYAQRDQLAKQVPMIKKKYPR
                     YAAYSKATIQEVFGDALSSAIKLETFVLNSSLFTNEKGKFTRTDLPNEAQIAPIRCII
                     IDDLNKDGNLDVLLSGNDRGFETETGVFDGCTGLILLGDGKGAFKPLPSRESGLWLTK
                     DARDMALIPSSNKSKILICGNNNGSTDVFQLK"
     gene            complement(3576..>3921)
                     /gene="pgk"
                     /locus_tag="PLE23_10955"
     CDS             complement(3576..>3921)
                     /gene="pgk"
                     /locus_tag="PLE23_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMX83826.1"
                     /translation="NMSIPEGMMGLDIGPQAISEFTRVITESKSILWNGPMGVFEMPN
                     FANGTNEIAKAVVEATGHGAFSLIGGGDSAAAINSMGLEDKVSFVSTGGGAMLELLEG
                     KELPGIREILED"
BASE COUNT          986 a          821 c          812 g         1302 t
ORIGIN      
        1 tatcaaatcc ccctttttga gcatccaaaa tgatccctaa tgaatttttt gggttaaatg
       61 aaagtgtatc tgcagagcgc aaagattgga aaaaagttca ggtgaatttt ctgccacctg
      121 aacttattta ctattgatat acttagcgag tattatttta gctgaaaaac atctgtactg
      181 ccattgttat tgccacatat tagtatttta cttttgttgg aagaagggat gagtgccata
      241 tctcttgcgt ccttcgtgag ccatagtccg gattcccttg aaggcaaggg tttgaaagct
      301 ccttttccgt cacccaaaag tataagaccg gtacaaccat cgaacacacc tgtttcggtt
      361 tcgaagcctc tgtcatttcc gcttaatagc acatcaaggt tgccgtcttt gttcaaatca
      421 tcaataatta tgcaccttat cggagctatc tgtgcttcgt taggcaggtc ggttcttgta
      481 aatttccctt tctcatttgt aaatagactg gagttgagta caaaggtttc gagtttaatg
      541 gctgacgata aggcatcacc aaatacttct tgtatggtag ctttggagta agcagcatat
      601 cttgggtatt ttttcttaat catcggtact tgttttgcca attggtctcg ttgagcataa
      661 ggaaagatgt tgccatttcg ggtaaaacac atgacagggt caatactgcc attgttatca
      721 aagtcttttg cgtacattac tatagggctc ttttcagagg cacggtgccg tgaatttttt
      781 cccagattgc cagctactat atccaggtca ccatctttgt caaaatcagc aattactata
      841 gagttccacc agccattggt tttttcaagg ccaaatgacg tgatagcatt cttataaact
      901 ttaccttgtt tcttaaatac ggttataggc atccattctc cgcacacgac tatttcgggt
      961 attttatcac cgtcaaggtc tgctgctttt atatccgaca ccatacccat ttgggagaat
     1021 tctggacaaa actctgctgt ggcatctctg agtacaccct tgtcattttt ataaatgaaa
     1081 cttttgggaa tggtagggaa tgcccccggt accactcttc cgccgatgag caaatcctgg
     1141 tctccgtcgc catctatgtc aaaaacgaga aaacgcccaa cattttgttt gaggtcttgg
     1201 atagcatttg tgttgcgttt aaaatttcct ttaccatcgt tggtataaat tcttagctgg
     1261 tagtagtctg tcccgtccgg attttcattt ccaccactgc ctactacaag gtcaaggtcg
     1321 ccgtcactat ccacatcaag gaatttgcta tctgtatctt cgcaatttct gtctttatca
     1381 aagtcgggtt gaatggttcg tttaaatttt cctcccggca gttgtatgta cagcgctccg
     1441 ggttcgcctt gtttttcctg attgccggtg gctccaccaa tataaatgtc ttcaagcttg
     1501 tcgccattga catcaccgtg tgctatataa gggccaagat tggagtaata atgtggtata
     1561 aggcgttcgt agttgaagtc aatatattca ttttcagaat gcacgaaatc caacttgctt
     1621 gcttttgtaa tgtcttcaaa taatggtgcc ggtttgggca atacagttag tgcctgattg
     1681 cctgcctcag catattttag attcagtttt tgattggtat tgacattcgt aagtacttgg
     1741 gtctttccat caggccatcg tatctccaat ctttctattg atttttcatt gcccgtaccg
     1801 aaatgcagga taggctcact gcttgacata aatccatggt tggtgtacat ttcctgatac
     1861 tgcatctgac ctttgttgta gatccatgct ttagtgccta agccaaactt gtttttgtca
     1921 ggaccgctta gtttaatctg tagataggtg tggtctgcca gttggctggt tttgttttca
     1981 aaaataaatg ccggatcgag gatgttattg acgacgaggt caagatcgcc gtcaccatca
     2041 aggtcagcat aagcaacacc attggaaaag gatggttcac cggttttcca ggcattggtc
     2101 atgttttcga atgtaaggtc acctttattt ctgaacatgt agttgggtag tttgtgttca
     2161 ggaatgatat tgagaaactc gtttacatcc gggaaacgtc ggggtgtaac gccaccggtt
     2221 ttctgaatag agtcacgtgt ataaaccata tagtcattgt cagttacatc gtgtcggtat
     2281 ccgtttgtta tgaaaatatc tttgttggtg tcgttgtcaa aatctgcaaa cacacaaccc
     2341 cagctccagt cggtattgga tatacctgcc atttgcccta tttcagaaaa gaagtcgtta
     2401 ccttgattga gctgtagcat attccgcgag gcttggtgcc cgtatccgta ttgaacaagc
     2461 aggttatacc tttcgtattg catgatgttc ataagctgtt tgtatctgac ctggtcttcg
     2521 gatatcatat caagggtcat gatgtcgggc agaccatcat tgttgaagtc ggcgacatcg
     2581 ctacccatac tgttttgtga actgtgtcgg agaatctgtg agaatttgtc tgtaaagcca
     2641 cctttttggt tattgataaa tacaaaatct ggctctatat aatcattggc cacaaaaata
     2701 tcgggatagt tatccatatt tatatcagcg gctgtagcac ttaatgaaaa tgcagatgtg
     2761 ttgaggccaa tacttgcact tatgtcagtg tatttgccat tgtcattccg atatagtctg
     2821 ctcgattcga agggagtctg agcaaaggtt tcacgtgtta tttttccatc gtcagactgg
     2881 cgaagtctcg tttttgtggc ttggtcgaat cctacccggt gattcatcat gaacatatct
     2941 aagtcaccgt ccaggtcata atcgaaaaat acggcatggt ttgtattggc atttccagcc
     3001 agattatatt cctgagcctg attggtgaat gtgaggtctc tgttgttgat gtacagataa
     3061 ttgtcctttt tgccatcatc ttcccgagat gtacggcata catatatatc gagatatccg
     3121 tcgttgttga catcggccat tgttacgcct gttttgaatc cggcattggc agccactcct
     3181 gcacgatcgg taatatcctc aaacttcatg ttgcccttgt tgaggtagag tttgttggta
     3241 ccgaacgttg ctgtgaaata gatgtcgggc aatccgtcat tgttgatgtc acccaccgtt
     3301 acgccacctc cattgtagag atattcgtaa ttcaggatat tatatacata atcttccttt
     3361 aaatgatttg aaaatgtgac accagttttt tcaggcgata acaattcaaa tctttgcgga
     3421 ccggagggaa cagtattttc ctctggttgc gaattatttt tgcaggcaat catgccaaga
     3481 gtgatggcta caacagtaaa aatttttcga gacatagtgt tttgctaatt atattagaat
     3541 cattacgtac tgcgaaaaat tctgtttcac ttccgctaat cttctaatat ttctctaata
     3601 cctggcagtt ctttaccttc gaggagttcg agcatagcac cacctccggt tgaaacaaaa
     3661 ctgactttat cttccagacc catactgtta atagcagctg cgctatcacc tccacctatt
     3721 aaagaaaatg ccccatgacc ggttgcttct actacagctt tggctatttc atttgttccg
     3781 ttagcaaagt ttggcatttc gaagacgccc attggtccgt tccagagtat tgattttgat
     3841 tctgttatca cacgtgtgaa ttctgaaatt gcttgcgggc cgatatcaag acccatcata
     3901 ccttcaggaa tggacatatt g
//
LOCUS       DAOQQY010000270         3889 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11288_length_3889_cov_6.349244, whole genome shotgun sequence.
ACCESSION   DAOQQY010000270 DAOQQY010000000
VERSION     DAOQQY010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11288_length_3889_cov_6.349244"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..439
                     /locus_tag="PLE23_10960"
     CDS             146..439
                     /locus_tag="PLE23_10960"
                     /inference="COORDINATES: protein motif:HMM:NF021431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HMX83827.1"
                     /translation="MRVIAKKILREFCEKHSSCEQQLKAWYKEADKGTWEKLNELKNE
                     YPSVSILEDNRVCFNIKGNNYRLIVKINFDYQMMWIRFIGTHAEYDKIDANKI"
     gene            441..800
                     /locus_tag="PLE23_10965"
     CDS             441..800
                     /locus_tag="PLE23_10965"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMX83828.1"
                     /translation="MMKLKPIKTESDYNQALKRLEQIFDAKPETKEGDELEILGILIE
                     NYESEFFPIDLPDPIEAIKFRMEQLNYSQNDLAKVIESKSRASEILSKKRKLTIGMIR
                     KLTDKLHIPTEVLIQAY"
     gene            complement(1071..1445)
                     /gene="folB"
                     /locus_tag="PLE23_10970"
     CDS             complement(1071..1445)
                     /gene="folB"
                     /locus_tag="PLE23_10970"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HMX83829.1"
                     /translation="MGKLAIEGMKFYAYHGHYEAENIIGNNYVVDVYFDMDLERAGKS
                     DKIGDTVNYEVVYRICAETMEFQFELIEKVGQYILDEITQMLSEKKDRLSPVKVLVRV
                     RKMHPPLDGYVESTFVEMEAVC"
     gene            1676..2896
                     /locus_tag="PLE23_10975"
     CDS             1676..2896
                     /locus_tag="PLE23_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMX83830.1"
                     /translation="MDMKTSSARGTTTSSREDRYQHHDYYLMDELLTQDHLLARDAVR
                     SWVKQEVSPIIEDYANKAECPKHLFKGLAEIGAFGPSLPAEYGCGGMDEIAYGVIMQE
                     LERGDSGIRSMASVQGSLVMYPIYKFGSEEQKRHYLPKLASGEYIGCFGLTEPDHGSN
                     PEGMISNIVDDGDAYILNGAKMWITNSPVADIAVVWAKDEQGIIRGMIVEKGMKGFTA
                     PEIHGKWSLRASITGELVFEDVRVPKKNILPNIQGLKGPLSCLSKARYGIAWGVIGAA
                     LDCYDSALRYSQERIQFGKPIGGFQLTQKKLAEMITEITKAQLLTWRLGVLANEGRAT
                     PAQISMAKRNNVNMALEIAREARQIHGGMGITNEYPVMRHMMNLETVLTYEGTHDIHL
                     LITGMDVTGFNAFK"
     gene            2984..>3889
                     /locus_tag="PLE23_10980"
     CDS             2984..>3889
                     /locus_tag="PLE23_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83831.1"
                     /translation="MTKKLIFGILLLNLMSNFGYSQQHNTTSKPAEIFLDNPSFEDYP
                     HVGIRNSSVEGWYDCGRNFFPSESPPDIHPSPDPGQPYFNVRTTPYDGKTFLGMVARE
                     NNTWESVSQRLSEPLKPGKCYNFSIYLCRSDTYTSAVSNNRFDLVPFTRRIQLRIWGG
                     NTYCGKLELLDASPLVNNTAWTRFEFQFKPKAQVSYIMLEAFYETPILFPYNGNILVD
                     KASSIVEFNCNEPIAAAVKKPSPKAPAKPAQPKPATPVVAQTQPKQAESNLSSINSKD
                     LKEGQTIRIDKLYFAADSANFSKESL"
BASE COUNT         1283 a          742 c          769 g         1095 t
ORIGIN      
        1 atgtttttgt atttttggta aaatttttga aatatttttt tcacagactg ccgttggtag
       61 aaacctcacc tcaaaaaaat acataaattc attaatttta tttcccgtta tgggaactta
      121 ttattatctt tgtaaaaaat aataattgag agtaatagca aaaaaaatat tgcgtgagtt
      181 ttgtgaaaaa cactccagtt gcgaacaaca attaaaagcg tggtataaag aggctgataa
      241 aggaacttgg gaaaaattaa atgaattaaa aaatgaatat ccaagtgtaa gtattttaga
      301 agacaacaga gtctgtttta acataaaagg aaataattat cgtctaattg tgaaaataaa
      361 tttcgattat caaatgatgt ggattcgatt tatcggaact cacgctgaat atgacaaaat
      421 agatgcaaat aaaatttaaa atgatgaaat taaaaccaat aaaaacagaa agcgattata
      481 atcaagcttt gaaaaggctt gagcaaatat tcgatgcaaa accagaaaca aaagaaggtg
      541 atgaattgga aatcctaggt atcttaattg agaattatga aagcgaattc ttcccaatag
      601 acctcccaga tccaatcgaa gcaattaaat tcagaatgga acaactaaat tattcacaaa
      661 atgatttagc caaagttatt gaatcaaaaa gtagagccag tgaaattcta agcaaaaaac
      721 gaaaattaac gattggaatg attagaaaat taactgataa attacatata ccaacagagg
      781 ttttaattca agcatattga gtaaaaaaga agggcttctg ctaacaaaca aaaaggcaag
      841 agagcgggtg aacggctgcg tatcaacgga catgcaaaat tcatcttctg tgcttcgtat
      901 caacaacatt gttagatagc cccaccttcg ccaagctgca aaagcgtttt acatcatttt
      961 taggaatgcc aaatgcattt acccgaactg catctggtca acttagcctt agcgatccaa
     1021 ttggttatcc cgaaataatc acaaacgtaa agccttattg taataactgt tcagcataca
     1081 gcctccatct ccacaaaggt actctcaaca tagccgtcca agggcggatg catcttcctt
     1141 acacgaacca atacctttac aggagataat ctgtcctttt tttcagataa catctgcgtt
     1201 atttcatcca ggatgtattg acctaccttc tctatcagct caaactggaa ttccatcgtc
     1261 tcggcacaga tacgatacac aacttcgtaa ttgaccgtat caccaatttt gtccgacttt
     1321 cctgcccttt ccagatccat gtcgaaatac acatctacca catagttgtt gccaatgata
     1381 ttttcagctt cataatgccc atggtaggca tagaatttca ttccctctat agcgagtttt
     1441 cccattttca gttgacttaa tggcacaaaa tacgtagatt cttataattt tttgtctgat
     1501 tttggttcga gacataataa tcagatgaaa tatattttga aaataccatg tattgatagt
     1561 tgtcacaaaa tttttaaaat gattttgatt gattcgatat taaatattct gattatgatt
     1621 ttttagttag caactgacag tattttttat taattttgca cacaaatttg aaaaaatgga
     1681 tatgaagaca tcatcagctc gaggaaccac aacatcttca cgcgaagacc gttatcagca
     1741 tcatgattat taccttatgg atgaactcct cacacaagat cacctcttgg caagagatgc
     1801 tgtgcgatca tgggtaaaac aagaagtgag cccgattatc gaagattatg ccaacaaggc
     1861 cgagtgtcct aaacatttat tcaaaggtct ggcagagata ggtgcctttg ggccgagcct
     1921 tcctgccgaa tatggttgtg gcggcatgga cgaaatagcg tatggtgtaa tcatgcagga
     1981 acttgaaaga ggcgactcgg gtatccgttc aatggcatcc gtacaaggtt cgctggtgat
     2041 gtatccgatt tataaatttg gatcggaaga acagaagaga cattacctgc caaagttagc
     2101 aagcggcgaa tatataggct gtttcgggct tacagagcca gatcatggat caaatccgga
     2161 aggtatgatc agcaatattg ttgatgacgg tgacgcttac atactcaacg gggccaagat
     2221 gtggataacc aatagccctg tcgctgatat tgcagtagta tgggcaaaag acgaacaagg
     2281 catcatcaga ggaatgatag ttgagaaagg gatgaaaggt ttcacagctc cggaaattca
     2341 cggcaaatgg tcgcttcgtg cttccattac cggtgagctc gtgttcgaag acgtgcgtgt
     2401 gccaaagaaa aacattcttc ctaatattca agggttgaaa ggaccgctca gttgtctgtc
     2461 aaaggcgagg tatggtattg cctggggcgt tataggtgct gcacttgatt gctacgattc
     2521 ggcattgaga tactcgcaag aacgcataca gtttggcaaa ccaatcggtg gatttcagct
     2581 tacacaaaaa aaacttgcag aaatgatcac cgagatcacc aaggcacagt tgcttacctg
     2641 gaggcttggt gtactggcca atgaaggcag ggcaacaccg gcacagatat ccatggccaa
     2701 gcgcaataac gttaatatgg cactcgaaat agcaagagag gcaagacaga tacacggagg
     2761 catgggtatc accaacgagt atcctgtaat gcgacacatg atgaatctcg agacagtgct
     2821 tacctatgaa ggtacacacg atattcattt gcttattaca ggtatggatg ttacaggctt
     2881 caatgcattt aaataattgt taaagtgctg ttgtactgaa atatccggtg cgatttctac
     2941 attacacttg tggtgcattt tatttaaaat attttgtcct tacatgacga aaaaactgat
     3001 ttttggaata ttattattga acctaatgtc caactttgga tattcgcagc aacacaatac
     3061 tacttcaaag cctgcagaaa tatttttgga taatccgtcg tttgaggatt acccacatgt
     3121 aggtatccgt aatagttctg ttgaaggttg gtatgattgc gggagaaatt tttttcctag
     3181 cgaatcgcca cctgacatcc atccttcacc tgatcccggc caaccatatt ttaatgttag
     3241 aacaacgcct tatgacggca aaacattttt ggggatggtt gcacgtgaga acaatacatg
     3301 ggaatcggtc tcacaaagat tgtctgaacc attaaaaccg ggcaagtgtt acaatttttc
     3361 aatatatctc tgccggtcgg atacatatac aagtgcagtc agcaataaca ggtttgattt
     3421 ggtgcctttt acccgccgca tccaactccg catttgggga ggaaatacct attgtggcaa
     3481 gctagagctt ctggatgctt caccactggt gaacaataca gcctggacca gatttgagtt
     3541 ccagtttaag ccaaaagcac aagtgagtta tattatgttg gaagcatttt acgaaacacc
     3601 tattttgttt ccatataacg gaaatatttt ggtggacaaa gcttcctcta ttgtagaatt
     3661 taattgcaat gaacctatag ctgcagcagt taagaagccc agtccaaaag caccggctaa
     3721 accagctcag ccaaaacctg ctactccggt agtcgcacag actcaaccta aacaagctga
     3781 atccaattta tcttccatca attcaaaaga tttaaaagag gggcagacta tcaggataga
     3841 caagttgtac tttgctgcag attctgcaaa tttcagcaaa gaatcgctt
//
LOCUS       DAOQQY010000271         3864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11399_length_3864_cov_6.281439, whole genome shotgun sequence.
ACCESSION   DAOQQY010000271 DAOQQY010000000
VERSION     DAOQQY010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11399_length_3864_cov_6.281439"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..1680
                     /locus_tag="PLE23_10985"
     CDS             310..1680
                     /locus_tag="PLE23_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMX83832.1"
                     /translation="MRFVDRKQELLRLKNTTNRPTSTLTIIYGRRRIGKSRLIKEVLT
                     TNDIYFMADQSETSRQIELFAKQISLHIKGFDKLVYPNWEAFFENLNYRVKKGTVICI
                     DEFPYIVKNSSELPSILQKIWEEKGKLNYHLILCGSAQQLMHNLVMNAAAPLYGRADE
                     IIKIKAIPAFYLQKILNTNAIDTITEYSIWGGIPRYWELRTKEDDLLSALNYHVFSSH
                     GILYNEPVHLFLDDMRDTVHSFTLLSLIASGSHRLSEIASRIEKPATHLSAPLNKLIQ
                     LGYIERETPFDENEKNSKKSLYKISDPFMDFYFNFVVPNRSLIEIGQTANVNKLILAQ
                     LHQYVSTHWERICRKAVPFLNIDNIVFKSASRWWGTPNKESEIEFDVVAESVDKKYLL
                     VGECKWNDTEINQTELTNVLLKKAVQLPFAKKKKIITALFLKTRQDLASEHVYTPQNI
                     IDAFEV"
     gene            1852..2679
                     /locus_tag="PLE23_10990"
     CDS             1852..2679
                     /locus_tag="PLE23_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX83833.1"
                     /translation="MKTNHLIIEAGRTEKNYWADLWRYRDLFYILSWRDIKVRYKQTV
                     VGVLWSVLRPLLTMIIFTFVFSNIAKLPAEGSAPYAIMVYAAMLPWQFFSNALSEASN
                     SLVGNANLISKVYFPRLIIPTASVITSFVDFLISFVILVGLMIYYMYAPSWNILLLTV
                     FLVIAFLASMGLGLYLTALNVKYRDFRYIVPFLVQFGLYISPVGFSSSVVPDQWRLLY
                     SLNPMVGVIDGFRWCILGGQSHIYWPGFLLSLGIVVLFLFLGVYQFRKMEKTFADVI"
     gene            2843..>3864
                     /locus_tag="PLE23_10995"
     CDS             2843..>3864
                     /locus_tag="PLE23_10995"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMX83834.1"
                     /translation="MTTNAIIPFVATHPGTLIRDELEVRDDITQTELATLLGVKPSFL
                     NEIIRGKRPVTADISILLEKALGIPADYWMRFQSQYEIDTARAKEKNKSKLKLIDIWN
                     TVSLYVPVKEFKKKGYLGNELSSNIAIIQDIYDVQSLEELEYKYKDKKFAFFKKSTKL
                     QVDDKNMIAWIALVEYDAGKKLTNSFELDKLSQLKKELQEIFYNNVDIIISVEEVLTQ
                     YGVKFVLVDKLEKTPIDGYSFWSENNPVIALTLRHSRIDNFAFTLLHELGHIALHLAR
                     YKEEKFLDLTEMDENETEHAANTYTQDALIPKECWEELKHNNAALTDEKIYEFSDKYR
                     IHPAIIL"
BASE COUNT         1255 a          784 c          770 g         1055 t
ORIGIN      
        1 ccattaagaa gttaagagaa ttaagcaggt acttcgcttg ggcaagcgga aagggtagca
       61 aaaaatctcc gttttgtctg ccccttccct caagggtgca aaacgaagat ttttgaatga
      121 aaattcgcac cacttaccat ttaccattca ccacttacca tttaccattc accattcacc
      181 ggtaaagacc ggcttgaggt cagcatagcc tgagcgggtg gtttgaagtc cgtactagtt
      241 caaacatcaa aaaaatcaaa ataattatgt ttataaaaca ttatctttgt aaaacataat
      301 taagtgacaa tgagatttgt tgacagaaaa caggaattgt taaggcttaa aaatacgacc
      361 aatcgcccta cttctacctt gaccatcata tacggaagga ggagaatagg gaaatcaagg
      421 ctgataaaag aagtattaac gacgaatgac atttacttca tggccgacca gtctgaaacc
      481 agcagacaaa tagagctttt tgcaaaacaa atcagtttac atatcaaggg tttcgacaaa
      541 cttgtgtatc ccaattggga agcatttttt gaaaacctga attacagagt taaaaaagga
      601 actgtcatct gtatagatga gttcccgtac attgtaaaaa actcatcaga acttccgtcc
      661 atacttcaaa aaatctggga agaaaaagga aaactaaatt accaccttat attgtgtggc
      721 tccgcgcaac aacttatgca caatttggta atgaatgccg ctgctccgct ttatggccgg
      781 gcagacgaaa tcattaaaat aaaggccatt ccggctttct accttcaaaa gattttaaac
      841 acaaatgcaa ttgacaccat aaccgaatac agtatatggg gaggtatccc gagatattgg
      901 gaattaagaa cgaaagagga tgatttacta agcgcattga attaccatgt atttagctca
      961 catggtattt tatacaatga gcctgtacat cttttcttag acgacatgag agatacagta
     1021 cattcgttta ccttgctgtc gcttattgct tcgggcagtc atcgcctgtc cgaaattgcc
     1081 tccagaattg aaaaaccggc aacccatctc tctgcccctc taaacaaact aatacaatta
     1141 ggctacatag aaagagagac accatttgat gagaacgaaa aaaatagcaa aaaaagcctg
     1201 tacaaaataa gcgacccttt tatggatttt tacttcaatt ttgtagtgcc caatcgttcc
     1261 ctgatagaaa ttggacagac ggcgaatgtc aacaaactga ttttggcaca gctgcaccaa
     1321 tatgtttcga cacattggga aagaatatgc cgaaaggcag ttccgttttt aaatattgac
     1381 aacatagtat ttaaatctgc ctcaagatgg tggggaacgc ccaataagga atccgaaatt
     1441 gaattcgatg ttgtagccga atctgttgat aaaaaatact tgttggttgg agaatgtaaa
     1501 tggaacgata cagaaattaa tcaaacagaa ctcaccaacg tactacttaa aaaagcagta
     1561 cagttaccct ttgctaaaaa gaaaaagatt atcactgcac tttttttaaa aaccagacag
     1621 gatttagctt cagagcacgt ttatacccca caaaacatta ttgatgcgtt cgaagtttaa
     1681 gagccttaaa tgatcagaaa acttacccga cacaataaaa tcccaaaggg atgacatgat
     1741 tgtagaaaaa acaatcgaag atggacaaac cctgaagggg tggcatgatg aggaactgca
     1801 aagccaccta agtcagcaaa gcaaaaacct atatcactaa aataaagaat attgaaaacc
     1861 aaccacctta tcatcgaagc cggccggaca gagaaaaact actgggccga cctctggcgt
     1921 taccgcgact tgttctacat acttagctgg cgcgacatca aagttcggta taagcagacc
     1981 gtagtcggtg tactgtggag tgtactccgc ccattgctca ctatgatcat ctttacattc
     2041 gtgttcagca atatcgccaa acttcctgcc gaaggctctg ccccttatgc tatcatggtc
     2101 tatgccgcta tgttgccttg gcagttcttc tccaacgccc ttagcgaggc cagcaatagc
     2161 ctcgtcggca acgccaacct cattagtaag gtctatttcc cgcggcttat cataccgaca
     2221 gcctcagtca tcaccagctt cgttgatttt ctcatatcct tcgttatcct tgtcggactt
     2281 atgatatatt atatgtacgc acccagctgg aacatcctcc tgcttaccgt attccttgtc
     2341 atcgctttct tggcaagcat gggccttggg ctatacctta ctgcactcaa tgtcaagtac
     2401 cgcgacttta ggtatatcgt gccgtttttg gtacagttcg gcttgtatat ctctccggtc
     2461 ggcttcagca gcagtgtcgt acccgaccaa tggcgactgc tctattcgct caacccgatg
     2521 gtaggcgtca ttgacggatt cagatggtgt attctcggcg gtcaaagcca tatatactgg
     2581 ccaggatttc tccttagtct tggtatagtg gtgctcttcc tcttccttgg cgtctatcag
     2641 tttcgcaaga tggaaaaaac ttttgctgac gttatataac aacgagtaca aataccggct
     2701 gttaaaatgt ttgcaaactt tacctggagc agcttaaagt tttttgaatt catatcttcc
     2761 ttgaaaactg aaggaacagt cacttttaca atatgtgaaa ttgtagattt aagtaaccac
     2821 tattcttaaa aaatcgaagg tgatgaccac aaatgccata ataccatttg tagcaacaca
     2881 cccgggtacg ttgatcaggg atgagttgga agtacgcgat gatattacac aaacagagtt
     2941 ggccactcta ttgggtgtta agccttcatt tttaaatgaa atcatcagag gtaaaagacc
     3001 tgttacggca gatatctcta ttctgttgga aaaagcactc ggtatcccgg ccgattattg
     3061 gatgaggttt cagtcacagt atgaaataga tactgcaaga gctaaagaga agaacaaaag
     3121 caaattaaaa ttgatagata tctggaatac tgtaagcttg tacgttcctg taaaagagtt
     3181 taagaaaaaa ggctatctgg gcaatgagct ttcttcaaat attgccatca tacaagacat
     3241 ctacgatgta cagtcgttgg aagaattgga gtataagtat aaagacaaaa aatttgcctt
     3301 cttcaagaag agtacaaaac tgcaagtgga cgataaaaat atgatagcct ggattgctct
     3361 ggtggaatat gatgccggga agaagttgac gaatagcttc gagttggaca aattgtctca
     3421 attgaaaaaa gaactgcaag aaatttttta caataatgtt gatataatca tatctgttga
     3481 agaagtcctg acacagtatg gagttaagtt cgtgttggtt gacaaattgg agaaaactcc
     3541 aatagatggt tattcgtttt ggtctgaaaa taatcctgta atcgctttga cattgcggca
     3601 tagcaggata gataattttg cctttacact cttgcacgaa ttgggtcata ttgcattgca
     3661 cttagcccgg tataaagaag aaaagttcct tgatttaacg gaaatggatg aaaatgaaac
     3721 agagcatgca gctaatactt atactcagga tgctttgatt cctaaggaat gttgggaaga
     3781 gctaaaacac aacaacgcag ctttgacgga cgaaaagatt tatgagttct cggacaaata
     3841 ccggatccat ccggcaatca tcct
//
LOCUS       DAOQQY010000272         3846 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11481_length_3846_cov_4.936428, whole genome shotgun sequence.
ACCESSION   DAOQQY010000272 DAOQQY010000000
VERSION     DAOQQY010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11481_length_3846_cov_4.936428"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2407
                     /locus_tag="PLE23_11000"
     CDS             <1..2407
                     /locus_tag="PLE23_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HMX83835.1"
                     /translation="AFVRIYAQPVPEFHPCHNAGKTYNRAKITPEEQKSILAGIERSD
                     TFDILHYNIYIDATNYSGKILKGKTKVLFKAKKDNLNKLRLDFTKLGVTKVEREGDAE
                     LNYNYTDPIIDIQLNKALDAGDIDSVTITYEGTPYQDPVWGGVYFASNYIYNLGIGLS
                     SNPPNFGRVWYPCFDNFVERATYDIKVLTNSGRKAYCVGTFIDQVKLGGDSIVRHYRM
                     NQLLPTYLTNFAAANYAEVNFTHTGQFGDVPVQLVAKPGDTTNLKKSFSNIGADVDAM
                     EKWFGKYAWERVGYVLTTVGAMEHPTNIAYPDNVIDNGNKSSETLMAHEFGHNWWGNI
                     VSPKTEFDMWIKEGNAEYCSHLFLEHVYGEDKFINAVKTNHLNVMRQAHQADGKYEPL
                     SGISFENIYGRHTYWKGADVMHNLRGYMGDSLFKVGMRSILDKYAYSAIDAIQFRDQL
                     TSSTGLDMKSFFDDQIFQPGFAGFEIDSVNYNIENNVQTAEIFVTQKLRKANHFYTNV
                     PLEITFYAKDWSKLTRKIVVSGEHSTATVELPKDFSVQYAILNEDNKLNYARMHANSK
                     LKLKAPVTMPYVEFSFKVNEIKDTALLSVQHMWIPPDPIKNNPDNAKISNSHYWIVDG
                     LLPDNFQASATLEYKTNTSNTFLDADLVSVTEDSLILVYRSSPKSDWRKYPYYTKQML
                     SPTDGKGFIKIDTLLLGEYAFANGSLPVVATHELRDEVNTLLVYPNPTGNLLNIPGQG
                     LTKGNKMIRIYDAAGKAVFDKEVYYNGNTMTINTSILATGQYVLSIFDGKRTEGKAIL
                     IKK"
     gene            2556..>3846
                     /locus_tag="PLE23_11005"
     CDS             2556..>3846
                     /locus_tag="PLE23_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMX83836.1"
                     /translation="MNRTLFCLLVVLLTINGIFAQNTLKGKIKGAADNEPLIGATVLI
                     KGTSEGANTDFDGIFELKTSRQVPFTIVVSYLGYDNVEVEVTSLAKLLDIRMEETAVQ
                     IGAVEVTGQRISDKQKESPLTVESMDLLAIKATPADNFYDGLGSMKGVDLTAASLGFK
                     IINMRGFNSTSPVRSLQIIDGVDNQAPGLNFSLGNFLGSSELDVNKVDLIVGASSAFY
                     GPNAFNGVISMETKSPFYHKGLSASLKFGERNLVETAFRYADALKNKNGKDVFGYKLN
                     FSYLRANDWEAENYNPVEGTLDKADNPGGYDAVNIYGDEFYSLNNNTSLVDYWGLTAG
                     LRRYYRKGYREADLVDYNTKNYKANLAFHIRTRPDKESASPEFIISSSFGSGTTVYQG
                     DNRFSLRNILFFQNRLEYRLKDKFFIRAYATNEDAGDS"
BASE COUNT         1227 a          787 c          809 g         1023 t
ORIGIN      
        1 tgccttcgtc agaatatatg cacagccggt accggagttt catccttgtc ataatgccgg
       61 taaaacttat aaccgagcaa aaattacacc tgaagaacaa aaatctatac tggcaggtat
      121 tgagcggagc gatacttttg atatccttca ctataatata tatatagatg ccaccaatta
      181 ttcaggaaaa attctgaaag ggaaaaccaa agtactgttt aaggcgaaaa aggataatct
      241 gaataaactc cgccttgatt ttacgaagct tggtgttaca aaagtagaac gcgaaggtga
      301 tgcagaactt aattataact ataccgaccc aattattgat atacagctta acaaagcact
      361 cgatgccggt gatatcgatt ctgtcaccat aacttatgag ggtacacctt atcaagaccc
      421 ggtatggggc ggtgtttatt ttgcaagtaa ttacatatac aacctgggta taggcctgtc
      481 ctccaatcca cccaacttcg gccgggtgtg gtacccttgt tttgataatt ttgtagaaag
      541 ggccacgtat gatatcaagg tgcttaccaa ttcgggaagg aaggcatact gtgtaggcac
      601 gttcattgac caggtcaagc tcggcggtga cagtatagtg cgtcattacc gaatgaacca
      661 gcttttgcct acttacctga ccaattttgc ggctgccaat tatgcagagg ttaattttac
      721 acataccggt cagttcggag atgtaccagt acaactggtt gctaagccgg gcgatactac
      781 caacctaaaa aaatcctttt ctaatatcgg tgctgatgta gatgcaatgg aaaaatggtt
      841 tggcaaatac gcatgggaac gtgtaggtta tgtgcttacc accgtaggtg caatggaaca
      901 ccctaccaac attgcatacc ccgacaatgt aatcgacaat ggcaataaat cgagtgaaac
      961 actaatggct cacgaattcg ggcataactg gtggggcaat attgtttctc caaaaactga
     1021 attcgatatg tggattaagg aaggaaatgc cgagtattgt tctcacttgt tcctcgaaca
     1081 cgtatatggt gaagacaaat tcatcaatgc cgttaaaacg aatcatctga atgtcatgcg
     1141 tcaggcccac caggcagatg gcaagtacga accactctcc ggcatcagtt ttgaaaacat
     1201 ttatggcaga catacttact ggaaaggagc tgatgtcatg cataacctcc gaggttacat
     1261 gggcgattca ctctttaagg taggtatgcg ttccattctc gacaaatatg cctattcggc
     1321 catagatgcc atacagttca gagatcagct gacaagctca accggactcg atatgaaatc
     1381 cttttttgac gatcagattt ttcagcccgg attcgcaggt tttgaaattg attctgtcaa
     1441 ttataacatt gaaaacaacg tacagaccgc agaaatattc gttacacaaa aacttcgaaa
     1501 agccaatcat ttctacacaa acgtaccttt agaaattaca ttttatgcaa aagattggtc
     1561 aaaactgaca agaaaaattg tagtgtcggg cgaacattca actgcaacag tcgaacttcc
     1621 aaaagacttt agtgtacagt atgccatatt gaacgaagac aacaaactta attatgcaag
     1681 aatgcatgcc aactcaaagc ttaagttaaa agctccggta acaatgcctt atgtagagtt
     1741 ttcatttaag gtaaacgaaa taaaggatac tgcacttttg tctgttcaac acatgtggat
     1801 accacccgac ccgatcaaaa ataatcctga caatgcgaaa atttccaatt cacattactg
     1861 gatagttgac ggccttttgc cggataattt tcaggcaagt gccacattgg agtataaaac
     1921 caacacaagc aataccttcc tggatgcaga cttggtatca gtgacggaag atagtctcat
     1981 cctcgtgtac cgctcatcac cgaaatctga ctggcgaaag tatccgtatt atacaaaaca
     2041 aatgttgagc cctacagatg gcaaaggttt tatcaaaata gacaccttgc tgctcggtga
     2101 atatgcattt gccaatggtt cactccctgt cgtggcaact cacgaactta gagatgaagt
     2161 aaacacgctt cttgtctatc caaacccaac gggcaatctg ctcaacattc cggggcaagg
     2221 gctaacaaaa ggcaacaaaa tgatcagaat atatgatgct gccggtaaag ctgtattcga
     2281 caaagaggta tattacaatg gaaatacgat gaccatcaat acctccatat tagcaaccgg
     2341 ccaatatgta ttgagtattt ttgatggtaa aagaacggaa ggaaaggcca tattgattaa
     2401 aaaataaaaa aatctggcgg aatgtaacat tatcatttcg ataaaaaaca gaatgcgacg
     2461 aaatatgtct taatttttat tacaatatgt ttgtaattga aaaaaaagtg atattttgcc
     2521 gagtaatttg tgtttaacta ataaaaatca attcgatgaa cagaacgctt ttctgcctct
     2581 tggtagtatt attgacgatc aatggcatat ttgctcagaa taccctgaaa gggaaaataa
     2641 aaggtgcagc ggacaacgaa ccgcttatag gcgctacggt tctcatcaaa ggtacatctg
     2701 aaggtgccaa caccgacttt gatggaatct tcgaactcaa aacatcgaga caagtaccat
     2761 ttacgattgt agtgtcttat ttgggctatg acaatgtcga ggttgaagtc acatctctag
     2821 caaaactact cgatattaga atggaagaaa cagcggtaca gataggtgcc gttgaagtaa
     2881 ccggacagcg gatatccgac aaacagaaag agtcaccact gacggtcgaa tcaatggacc
     2941 tcttggcgat aaaggctacg ccggccgata atttctacga tgggttgggc agcatgaagg
     3001 gcgttgacct cacagcggcc agtcttggtt ttaagatcat caatatgcgc gggttcaaca
     3061 gcacgagccc tgtaagatca ttgcagataa tagacggtgt agataatcag gctccgggtc
     3121 tcaacttttc acttggcaac tttttgggca gctcagaact cgatgtcaac aaggttgatt
     3181 tgatagtagg tgccagttca gccttctatg gcccgaatgc tttcaatggc gttatcagta
     3241 tggagaccaa gagcccgttc tatcacaaag gtctttctgc aagtctgaaa tttggcgaaa
     3301 gaaatctggt agagactgca tttcgttatg ccgatgcttt gaaaaacaaa aacggaaaag
     3361 acgtatttgg ctacaaactc aatttttctt atctgagagc aaacgactgg gaagcagaaa
     3421 actacaatcc tgtagaaggt acgcttgaca aggccgataa cccgggcgga tatgatgctg
     3481 tcaacattta tggtgatgag ttctattctc ttaacaacaa cacttcgctt gttgattatt
     3541 gggggcttac cgccggattg agaaggtact accgcaaagg ttaccgagag gctgatctgg
     3601 tcgattataa tacaaaaaat tacaaggcca atctggcatt ccacattcgt accagacccg
     3661 ataaagaatc tgcatcacca gaatttatca tttcatccag ttttggttca ggtaccactg
     3721 tttatcaagg cgataaccgt tttagccttc ggaatatatt gtttttccaa aaccgtcttg
     3781 aataccggtt gaaggataaa ttctttatca gagcgtatgc tactaacgaa gatgctggcg
     3841 attcgt
//
LOCUS       DAOQQY010000273         3819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11606_length_3819_cov_5.181722, whole genome shotgun sequence.
ACCESSION   DAOQQY010000273 DAOQQY010000000
VERSION     DAOQQY010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11606_length_3819_cov_5.181722"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="PLE23_11010"
     CDS             <1..838
                     /locus_tag="PLE23_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HMX83837.1"
                     /translation="GSLKMDQFKKENPERFIQVGIAEANMMGIAAGLATCGKIPYTGT
                     FANFSTGRVYDQIRQSIAYSDKNVKICASHAGVTLGEDGATHQILEDIGMMKMLPGMT
                     VINPCDYAQTKAATIAIANHEGPVYLRFGRPSWPVFTENLPFEIGKGILLNEGKDVTI
                     VATGHLVWKAVEAVKQLENEGISCDLINIHTIKPLDEDIILRSVRKTGCIISAEEHQR
                     NGGLGDSIAQLLARNLPTPQEYVAVNDQFGESGTPDQLLEKYGMGTSDVVTAVRNVLK
                     RK"
     gene            873..2033
                     /locus_tag="PLE23_11015"
     CDS             873..2033
                     /locus_tag="PLE23_11015"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HMX83838.1"
                     /translation="MKHDKMKFETKVIHAGIEPDPSTGAIMTPIFQTSTYVQEEPGKH
                     KGFEYARTQNPTRKVLETNLAALEGGADAVCFGSGLAAMDAILKLLSPGDEIIATNDL
                     YGGSYRIIVRIYQKFGIKAHFINMEDTELVEKTINDNTKLIWIETPTNPMLKIIDIEK
                     LCSIAKKHNILSCVDNTFASPYLQQPISLGADLVLHSATKYLGGHSDVVLGAVICSTK
                     ELAEQLHFIQNASGAVNGPFDCFLVLRGIKTLHVRVKQACKNARKIAKFLKKHPKVSK
                     VYFPGLKDHPGHDIAKKQMKYFGAMLSFDLKADNYESAKAVLSNTKLFSLAESLGGVE
                     SLIGHPASMTHASIPREERMKVGLTDSLIRLSVGIEDADDLIQDLAEALDKV"
     gene            complement(2076..>3819)
                     /locus_tag="PLE23_11020"
     CDS             complement(2076..>3819)
                     /locus_tag="PLE23_11020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83839.1"
                     /translation="QPFVDMMRSARTWYTKDVGNPTGGPFDTGVKGQLSFRPDGYPTH
                     IPQTIPAMDYPQAVATIWGSTSGWEPGSYTLIHEGTGELLFWGAGVNVLHPTDSTYSF
                     EIVNPIGNVFEVRIESSDINDPIRNIRIVKNDYLTTYKTQPFNPIWLEKVKMFKSVRF
                     MDWGQTNNWHLNEPWDWEHPDKYGWEERAKPGEYTWATNRGIPYELMIKLMNDYNLDG
                     WVCVPHNVDDGYIQQMAQLFHDNLEPGRKLTVEYSNEIWNWIFGQAQWLNKYGCIDKG
                     VSWPEGTVPYIQNCMDIWSEVFKDDMSRITRAVGIQTGWLDVAQRTAFNMVPGSFDVV
                     AGTYYFGLSDYADQTLDNLGATATVQDVVGLVRSTWHESKQIIKSVQTELADSLGLPL
                     AFYEGGQHLTPNPFGVEPTYAQALLDVQRDTAIYNLYNEWFDFLRTLQKGDEPLQCMN
                     FSLIAERSARYGSWGIWETMFQDTTEIPAPKLKALIENIFNGCDDNTTLTHNRPLIHA
                     SLKCYPNPTTGEFVVIMENGGEIHITDILGRPIASFNAKYGENKLDARDWPQGIYLVR
                     TSDGQLGKMVKSAY"
BASE COUNT         1088 a          897 c          825 g         1009 t
ORIGIN      
        1 gggctcactc aagatggatc agttcaaaaa ggaaaaccct gagagattca ttcaagttgg
       61 aatagcagaa gccaacatga tgggcattgc cgccggactc gctacttgtg gtaaaatacc
      121 atatacgggc acatttgcca acttctctac cggccgtgtg tatgaccaaa tcaggcaatc
      181 tatagcttac tccgacaaaa atgttaaaat atgtgcatca catgcaggtg ttacgctggg
      241 tgaagatggt gccacccacc agatactcga agacatcggt atgatgaaga tgctacccgg
      301 tatgaccgtc atcaatccgt gcgattatgc acagacaaag gcagccacca tcgccattgc
      361 aaatcatgaa ggtcccgttt accttcgatt cggtcggccc tcatggccag tttttacaga
      421 gaatcttcct ttcgaaatcg gtaaaggcat actgctcaac gaaggaaaag acgtgaccat
      481 cgtagccacc ggacatctgg tttggaaggc cgtagaagcg gtaaaacaac tggaaaacga
      541 aggaatcagc tgcgatctga tcaacatcca caccatcaaa ccactggacg aagacattat
      601 cctccgttcg gtcaggaaaa ccggatgtat tatctctgcc gaagaacatc aacgcaatgg
      661 aggtctcggt gacagtatcg cccaattgct ggcaagaaat ttgccaacac cacaagagta
      721 tgttgccgtt aatgatcagt tcggcgagag tggtacaccc gatcagttgc ttgagaaata
      781 cggtatgggc acaagcgatg tagtaactgc cgtaagaaat gttttgaaaa gaaaatagat
      841 tcagatttca ccaaaaaaat aaatcgcctt acatgaagca cgataaaatg aaattcgaaa
      901 ccaaggtaat acacgcagga atagagcccg acccatcgac cggagccatc atgacaccaa
      961 tcttccagac ttccacctat gtacaggaag aaccgggaaa gcacaagggg ttcgaatatg
     1021 cacgaacaca gaatccgacc agaaaagtgc tggaaaccaa cctcgctgct ctcgaaggcg
     1081 gtgctgatgc agtctgcttt ggaagtggtc tggcagcaat ggatgccatt ctgaaattat
     1141 tgtcgccagg tgacgaaatt atagctacca acgacctcta tggcggatcg tacaggatta
     1201 ttgtgcggat ataccagaag tttggcataa aggcccattt tatcaacatg gaagacacgg
     1261 agctggtaga gaaaaccatc aatgacaata ccaaactgat atggatagag actcctacca
     1321 acccaatgtt gaagattata gatattgaaa aactttgttc tatagctaaa aagcacaata
     1381 tactgagctg tgtggataac acatttgcat cgccatatct tcagcagccg atttcgcttg
     1441 gggctgacct ggttcttcat tctgctacca aatacctcgg gggccactct gatgtagtgc
     1501 ttggtgccgt catttgctct accaaagaac ttgcagagca gttgcatttc atccagaatg
     1561 ccagtggagc ggtcaacggg ccgttcgact gttttcttgt actcagaggt atcaaaacgc
     1621 tacacgtaag ggtaaaacaa gcctgcaaaa acgcacgtaa aattgcgaaa ttcttaaaga
     1681 aacatccaaa agtatctaag gtgtattttc caggtctcaa agatcatccg gggcatgata
     1741 tagccaaaaa acaaatgaaa tacttcggcg ccatgctctc cttcgatctg aaagccgata
     1801 actacgaaag cgctaaagct gtgctcagca atactaaatt attctcgctt gcagaatcgc
     1861 tcggtggtgt agaatcgctc atcggtcacc cggcaagcat gactcatgcc agtattcctc
     1921 gtgaagagag aatgaaagtt ggtcttacag attctctgat acgcctgagt gtaggtatcg
     1981 aggatgcaga cgaccttatt caagatctgg ccgaggcact ggataaggtg taggcaatgt
     2041 accagatgac attttaccca taataaaatc catctttaat aagctgattt taccatctta
     2101 cctaattgtc cgtctgaagt tcggacaagg tatattcctt gtggccaatc tctggcatcc
     2161 agtttgtttt ctccatattt tgcattgaaa ctggctatag gccttcccag tatatcggtt
     2221 atgtgtattt cgccaccatt ttccataata actacaaatt cgcctgttgt tgggtttgga
     2281 tagcatttta gactggcatg tattaatggg cgattatgtg ttagtgtagt gttatcatca
     2341 cagccgttaa aaatattttc aattaatgcc ttcagcttgg gggcaggtat ctcggtcgta
     2401 tcctgaaaca tggtttccca gatgccccag ctaccatagc gggcactacg ttcggctatg
     2461 agagagaaat tcatacattg aagtggctca tcgccctttt gtaatgttcg cagaaaatcg
     2521 aaccattcat tatacagatt gtaaatggct gtatcacgct gtacatctag taaagcctgt
     2581 gcatacgtag gttctacacc aaatggatta ggtgtcaggt gctgaccgcc ttcataaaac
     2641 gccaaaggta gtccgagcga atcggcaagc tcggtctgta cacttttaat aatctgcttc
     2701 gattcgtgcc aagtagaacg aaccagacct accacatcct gcactgtagc tgttgctccc
     2761 agattatcga gtgtttgatc tgcatagtca ctcaaaccga aatagtaagt accggccacc
     2821 acatcaaaac tacccggtac catattgaat gcagtacgct gtgcaacgtc aagccaaccg
     2881 gtttgtatgc ctactgcacg cgttatccga ctcatatcat ccttgaacac ttcactccag
     2941 atgtccatac aattctggat gtagggtact gttccttccg gccagcttac tcctttgtct
     3001 atacaaccgt atttgttgag ccattgtgcc tgaccgaata tccagttcca tatttcgttc
     3061 gaatattcta ctgtcagttt tcgcccgggc tctagattat catgaaaaag ttgtgccatc
     3121 tgttgtatat aaccatcatc cacattatgt ggtacgcata cccatccatc cagattgtaa
     3181 tcattcatca gcttaatcat caactcatac gggatgcccc tgttggttgc ccaggtatat
     3241 tctccgggtt tggctctttc ctcccatccg tacttatccg gatgctccca gtcccaaggt
     3301 tcattcagat gccagttatt ggtttggccc cagtccataa acctgactga tttgaacatc
     3361 ttcacctttt ctaaccaaat cgggttaaaa ggctgggttt tataggtagt aaggtaatcg
     3421 ttctttacaa tcctgatatt gcggatcgga tcgtttatgt cagaactttc gatgcgtact
     3481 tcaaaaacat ttccgatagg gttgactatc tcaaagctat aagtactgtc tgtaggatgc
     3541 aagacattaa cacctgcgcc ccagaaaagc agttcgcctg taccttcatg tatcagagtg
     3601 tagctacctg gttcccaacc acttgtacta ccccaaatag tagcgacagc ctgtggataa
     3661 tccatagctg gtatagtctg tggaatgtgt gtcgggtaac catcagggcg aaacgaaagt
     3721 tgtcctttaa caccggtatc aaatgggcct ccggtggggt tacccacatc ttttgtgtac
     3781 cacgtacggg ccgatcgcat catatcaaca aaaggttgc
//
LOCUS       DAOQQY010000274         3809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11647_length_3809_cov_5.487214, whole genome shotgun sequence.
ACCESSION   DAOQQY010000274 DAOQQY010000000
VERSION     DAOQQY010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11647_length_3809_cov_5.487214"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="PLE23_11025"
     CDS             <1..1570
                     /locus_tag="PLE23_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83840.1"
                     /translation="FPGWSIDCPYNGTYVDYNNDPITPQVKNVMSYFILNGCGTRDEF
                     TDCQYDKVAMIYDIYRKYQLSHAYEINYVNTVNFENTAQPVPNAVFEWKFADEPKKWR
                     STSDINGNMQGILYNPNVRITSYKLGSIVEQPQFNYDPWKLIPSYAEWIEKLSSYDLY
                     TLNNHIQKVKLLKNGYKKIAADLDKNHLIEPNDTKLLSNLLVGKIKKLDAYHSPYVFV
                     PEFIPLNYKTGFDANPFNMQINGQQVNNAEYIDPDFEFQISNGLAAGAGFDGVKISKV
                     SGENTAGNEELGGINPGLVNPMIQLYSDQLYKIDFRLQNSVSLSAYQLKFQIDTSIAS
                     IINYSHPTGTSLGDSLNTYFEGNQLITVWYNSTADSTNVSVTQNLLSLIILPNQNAST
                     SQILNIINARNATALYDAGGQLVENNLVMQATIFNPFQKETIIKNTESQSFIYPNPTS
                     GTVNMKFELQEAQQVEYYVFDTGGKLQIEQRLKLEAGKHDLAIDQVSNLGAGLYVVML
                     KTSGSDHSQIFIKH"
     gene            1583..>3809
                     /locus_tag="PLE23_11030"
     CDS             1583..>3809
                     /locus_tag="PLE23_11030"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HMX83841.1"
                     /translation="MKHIIRFVITAYILGFAGAVMVDAQNKIIYIKQDAKGKNDGSSW
                     EDAFSNFDSLFTNLEDGLEVWISKGEYTGGGKIEWKLSGYNVSIFGGFDGTETSIFDR
                     DIKKNETKINGYKQLNTLKFQNGRYLLDGLIIQNGKAKGLNIGPDLYDCEYWGPAVFS
                     CRGGGIHVYSDSVDKPCNLTIKNCIIRKNSALFGGGLYIDSTPGGVCGLLMDSCEVRE
                     NYAEEQGGGIYIIGGKAKQLPFIISNTIIESNASSNTGGILYASKDANGEFELRNCDL
                     SKNLANVIGGAAIGGHDNKRSKILNCRFMNNKGTNYNGGGLGAMLISTADVRNCVFNG
                     NIGWSGNFRARRTNFTNCVFAKNKSYYPEDNLFEIFEWGNPPPDERSNFYNCSFYDET
                     KGAKHLFPFIGQNVNFNNCAFEVTDTNLIFFKTVVDTVTFNYCSFNRKGLSDKFTAKP
                     VSADLLQINNCLWDTPAGFRDTISNDFRLSSCSPLINKGSNEYIAYLDSTDLSGDARV
                     QEDQIDIGAYETTGVQISYTTQANLCAVDSTGMITVTATGGLPVYGLEYNGVQHDTLI
                     LNNIQSGEQIIRFYDGTQCSIDIVAKFGPEHISFDTLIVDAGSKTSKDGAIKLSNIQG
                     GNPPLLFSWSPVISASDSIGGLEPGKYTLTVTDSAGCTEAFSFYVSYPDAVDEWLLQR
                     NIKLYPNPTSTMLTLELSTGLAHTSSYTIHCYDLLGMRCYTGTLPAYSYLHTIDVSAF
                     AP"
BASE COUNT         1233 a          678 c          867 g         1031 t
ORIGIN      
        1 ttttcccggg tggagcatag attgtccata taacgggact tatgttgatt acaataatga
       61 tccgataacg ccccaggtga aaaatgtaat gagctatttt attttgaatg gatgtggtac
      121 aagagatgag tttaccgact gccagtatga taaggttgcg atgatttatg atatatacag
      181 gaaataccag cttagtcacg cctacgaaat aaactatgtt aatacagtaa attttgaaaa
      241 taccgcccag ccggttccga atgctgtttt tgaatggaag tttgccgatg agccgaagaa
      301 atggcgcagt acgagtgata ttaatggaaa tatgcaaggt attttataca acccgaatgt
      361 aaggataaca tcatataaac ttggctcaat agtggagcaa ccacagttta actatgatcc
      421 ctggaaattg ataccaagct atgcagaatg gatagaaaag ttaagctctt atgaccttta
      481 tacgctaaat aatcacatac agaaagtaaa gttgctaaaa aacggttata agaagattgc
      541 agcggatctt gacaagaacc atttgataga gccgaatgac acgaaactgc tcagcaacct
      601 gttggtaggg aagataaaga aactggatgc ttaccactct ccttatgttt ttgtgccgga
      661 gttcataccc ttaaactaca agacagggtt tgatgcgaat ccttttaata tgcaaatcaa
      721 cggtcagcag gtaaacaatg ctgaatatat tgatcctgat tttgaatttc aaatcagtaa
      781 tggtctagct gccggtgccg gttttgacgg ggtgaaaatc agcaaagtgt caggcgagaa
      841 tacagcaggc aatgaagaac tgggaggtat taaccccggt ttggttaatc cgatgatcca
      901 gttatatagt gatcagctgt ataagattga tttcaggcta caaaattctg ttagtttatc
      961 agcatatcag cttaaattcc agatagacac aagtattgca agcataataa actacagtca
     1021 cccgacgggt acatccttgg gtgattcact gaatacttat tttgagggca atcagctgat
     1081 aacggtatgg tataattcta cagctgacag cactaatgtt tcagtcacgc agaatttatt
     1141 gtctttaata attctgccaa atcagaacgc gagcaccagc caaatactca atattataaa
     1201 tgcacgaaat gcaacagcac tctatgatgc gggcggtcag ctggtagaaa ataatctagt
     1261 aatgcaggca acgatattca atccattcca aaaagagacg attattaaaa acactgaatc
     1321 acagtcattc atttatccaa acccgacatc aggcacggtt aatatgaaat ttgaattaca
     1381 ggaagctcag caagtggaat actatgtgtt tgatacgggt ggtaaattgc agatagagca
     1441 aagactgaaa cttgaagccg gaaagcatga cctggcaata gatcaggtaa gcaatcttgg
     1501 agccggtttg tatgtagtta tgctgaaaac atcaggctca gaccattcac aaatatttat
     1561 taagcattaa ataagtcgtc tcatgaaaca tattatacgg tttgtaatca cggcatatat
     1621 tctgggtttc gccggagctg tcatggttga tgctcaaaat aaaattattt acatcaaaca
     1681 agatgctaaa ggaaaaaatg atggcagtag ttgggaggat gccttcagta actttgattc
     1741 attgtttact aatcttgaag atgggcttga agtatggata tcaaaaggag aatatacggg
     1801 aggaggtaag atagaatgga aactttcagg atataatgtt tcaatttttg gaggttttga
     1861 tggaacggag acaagcatat ttgatcggga tattaaaaag aatgaaacca agataaatgg
     1921 atacaaacaa ttaaatacgc ttaaatttca gaacggcaga tatttattgg acggattgat
     1981 aatacaaaac ggaaaggcaa aagggctaaa cattggacct gatttgtatg attgcgaata
     2041 ttggggtcct gcagtattca gttgccgggg tggaggaatc catgtttata gcgattcagt
     2101 tgacaagccc tgcaacctga cgatcaaaaa ctgtattata aggaaaaact cagcattgtt
     2161 tggtggcggt ctatacattg attccacgcc tggaggtgtc tgtggtttat tgatggatag
     2221 ttgtgaggtg agggaaaact atgcagagga gcaaggaggt gggatataca taataggcgg
     2281 gaaggccaaa caattgcctt ttattataag taatacgata atagaaagta atgcgtcgtc
     2341 taataccgga ggaattttat atgcgtcaaa ggatgctaat ggagaattcg aactccgtaa
     2401 ttgtgatctg tcaaagaatt tagctaatgt cataggtggt gctgcgatag gagggcacga
     2461 taacaaacgc tccaagattc ttaactgtcg ctttatgaat aacaaaggga caaattacaa
     2521 cggtggaggt ttgggtgcta tgttaatatc aacagcagac gtaagaaatt gtgtttttaa
     2581 tggtaatata ggctggagtg gtaattttcg ggcaagaaga accaatttca caaactgtgt
     2641 atttgcaaag aataagtctt actatcctga agacaacttg tttgagatat ttgagtgggg
     2701 aaatccaccg ccagatgagc ggagcaattt ttataattgc agcttttatg atgagacaaa
     2761 gggcgcaaag caccttttcc cgtttatagg gcaaaatgtc aattttaata attgtgcctt
     2821 tgaagtaacg gatacgaatc tgatattttt caaaaccgtg gtcgatacgg taacattcaa
     2881 ttattgtagt tttaaccgta agggattgtc agataaattt accgccaaac cggtatcagc
     2941 tgacttgcta cagatcaaca actgtctgtg ggatacacct gcgggcttca gagacacaat
     3001 cagtaatgat tttaggctga gcagttgttc gccattgatt aacaaaggct caaacgagta
     3061 tatagcgtat ctggattcga cagacctgtc tggagacgcc cgtgtacagg aagaccagat
     3121 agacataggc gcttatgaaa ctaccggagt acaaataagt tacacgacac aggccaatct
     3181 ttgtgcagtt gattctaccg gaatgataac agtgacagca acaggcggct tgccggtcta
     3241 tggtcttgag tataacggtg tacaacatga tacactgatc ttgaataata tacaaagcgg
     3301 agaacaaatt atccgattct atgacggcac acaatgcagt attgacattg tggcaaagtt
     3361 cggacctgaa cacattagtt tcgacacact gatagtagat gctggcagca agacgagcaa
     3421 agacggagcc ataaagctga gcaacataca aggaggcaac ccaccgttgc tattcagttg
     3481 gtctccggtg atatctgcat cagattcgat aggcggcctt gaacctggta aatacacctt
     3541 gactgttaca gacagtgcag gctgtactga agccttcagt ttttatgttt catatcctga
     3601 cgctgttgat gaatggttgc tccaaaggaa tataaagcta tatccgaatc caaccagcac
     3661 tatgctgacc ttggagttga gtacagggct tgcgcataca tcctcctaca ccatccactg
     3721 ctacgacctg ctgggcatgc gctgttatac gggcacgcta ccggcgtatt cgtacctgca
     3781 cacgatagat gtatcggcct tcgccccgg
//
LOCUS       DAOQQY010000275         3796 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11713_length_3796_cov_4.738840, whole genome shotgun sequence.
ACCESSION   DAOQQY010000275 DAOQQY010000000
VERSION     DAOQQY010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11713_length_3796_cov_4.738840"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLE23_11035"
     CDS             <1..237
                     /locus_tag="PLE23_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83842.1"
                     /translation="PRISRFVERIDMKIYDRWGVLVFETTHPEINWSGTDMQGKDLAE
                     GVYYYICTYFVNGQPTIIVKQNVLNGFIHLIKGQ"
     gene            242..838
                     /locus_tag="PLE23_11040"
     CDS             242..838
                     /locus_tag="PLE23_11040"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMX83843.1"
                     /translation="MFTGIIESTGSVEEIIREQSNVHFLIASNISAQLKVDQSVSHNG
                     VCLTVIEVGEYAHRVTAVQETLNRTSLGQWQTGGLVNLERCLQMGGRLDGHIVQGHVD
                     TVAECVYVNDKNGSHEFGFSFDERFAHLLVPKGSVCINGVSLTVNEPTLKQFHVDIIP
                     YTFENTAFRTLKAGDLVNIEFDIIGKYLARYQEILQNK"
     gene            841..1470
                     /locus_tag="PLE23_11045"
     CDS             841..1470
                     /locus_tag="PLE23_11045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83844.1"
                     /translation="MNHPAIYVIKILLTTLIVLGSAACNISIGQHLLLDRLTIGQSDG
                     KVYISCIVKAGNTCNGIVLYRSLNNVDYEVIDKILGVCGNITSPQAYDFVDKDPVKNA
                     VNYYKLELGGYGFSDVLSIHILDLSKDGLLVRPNPSNGMTTLNFKNESQGESVISLYD
                     SYGRTVFTKSTFDTSVQLDVSTYAPGMYYFGVLQDKKYFKTAGRLVVIR"
     gene            1577..2395
                     /locus_tag="PLE23_11050"
     CDS             1577..2395
                     /locus_tag="PLE23_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMX83845.1"
                     /translation="MKSTPNVWLVLTLLLIACQPKPIQYNDPVPDHESFTLASKILNE
                     TRTLNVWLPPQYKSGDAKYPVVYMPDGGIVGEDFPHIANTLAELISAGTIQPVILVGI
                     QNTNRKRDLTGPTVVAKDKEWIPENGGSDAFRRFIRDELFMEVNKRYRTTDTRTIVGE
                     SLAGLFVTETFLLAPDMFNNYIAIDPSLWWNDQYLVKNAGRLLATGDMNGRKFWFAGS
                     STKDIQEATSKLADALTKVVRPGLHWKYSPEPNEKHNTIFRAVKQKAFLWMLGK"
     gene            2534..>3796
                     /locus_tag="PLE23_11055"
     CDS             2534..>3796
                     /locus_tag="PLE23_11055"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HMX83846.1"
                     /translation="MPRIALFLAVICLFGNIARAQQSSQTQQLILQLKNEVADNNQQQ
                     GEISSIGHPVLDQLARDYQVLKINIYRAGIHTGKYFYTLSFPTDTDMDRLAEIFVHTG
                     EVRYAEPDYTGTSGGQLGTVPNDTYHNRQWGLHNTGNFSLAPSKAGADIEMHNAWDIS
                     QGSSDVVVAIMDSGIKLDHPELSGRIWQNTEEIAGNGLDDDNNGYADDTQGWDFVNND
                     NTPADDLGHGTNIAGIIGANGNNSIGYAGIDWHCRLMALKGLNSQNSGQYSWWSSALY
                     YAADNGAHIINLSVGGSGFSQTLKDAVDYCLDKGVCLVVCMMNTNTETPYYPAAFPGV
                     IAVGSTNPDDTRTQPFFWDAASGSNYGSHISVIAPGNYTYGLHHLSNTNYNTYWGGTS
                     QATPHVVGVAALLLAQDPTRTPADIKNII"
BASE COUNT         1103 a          915 c          834 g          944 t
ORIGIN      
        1 ccaagaatca gccgctttgt agaaagaatc gatatgaaga tatacgaccg atggggtgta
       61 cttgtgttcg aaactaccca tccggaaatc aactggtctg gtacggatat gcagggaaaa
      121 gacctggccg aaggtgtgta ctactacatc tgtacgtatt ttgtcaatgg tcagcccaca
      181 attatcgtta aacaaaatgt gctaaacggg tttatacatc taatcaaagg acaatgacgg
      241 tatgtttaca ggcatcatag aatcaaccgg tagcgtagaa gaaataatac gtgaacaatc
      301 gaatgttcat ttccttattg cttccaatat ttcagcacaa ctgaaggttg accaaagtgt
      361 gagccataat ggagtttgcc ttactgtcat cgaagtgggt gaatatgcgc accgtgtcac
      421 tgccgtgcaa gaaaccctca accgaaccag tctgggacaa tggcagaccg gtgggctcgt
      481 caacctcgaa cgctgcctgc agatgggtgg ccgcctcgac ggacatatag tacaagggca
      541 tgtagatact gtggcagaat gtgtgtatgt gaatgataaa aacggtagcc atgagtttgg
      601 tttttccttt gatgaaagat ttgcacacct tctggtgcca aaagggtcgg tttgcatcaa
      661 tggagtttca cttacggtca acgaaccaac actgaagcag tttcacgtgg atatcattcc
      721 ctatactttc gagaatacag cttttcgtac actgaaagcc ggtgacctgg tcaatatcga
      781 attcgatatc atcggaaaat accttgcacg ttatcaggaa atcttgcaaa acaaataaag
      841 atgaaccatc cggccatata tgttataaaa attttgctga caactcttat tgtactcggt
      901 tcggccgctt gcaatataag catcgggcag catcttctac ttgaccggct caccatcggc
      961 cagtcggatg gcaaagtcta tataagctgt atagtgaagg caggcaatac atgtaacggg
     1021 atagttttgt accgctcttt gaacaatgtt gattatgaag tgattgacaa gatactcggt
     1081 gtctgtggca acatcaccag cccacaagct tatgattttg tagataaaga ccccgtgaag
     1141 aatgccgtaa attattataa actcgagctc ggcgggtacg ggttttctga tgtattgagc
     1201 atccatatcc ttgacctgag taaagacgga ctactggtgc gccccaatcc ttccaatggc
     1261 atgactaccc tgaatttcaa aaatgaatcg cagggcgaat ccgtcatcag tttgtatgat
     1321 tcgtatggcc gcactgtttt tactaaaagc acttttgaca cttcggtcca gctggatgta
     1381 tccacttatg caccgggtat gtattatttt ggagttctgc aggataaaaa atatttcaaa
     1441 accgccggcc ggcttgtagt aatccggtga tccggtggca atcatttgcg tccgttaaac
     1501 aaaaaatcaa aaaccaccgc tgagctgaat atttaactga acaaacctat tattaattga
     1561 accaaataat tattacatga aatctacgcc caatgtatgg ctcgttttga cattactact
     1621 tattgcatgc cagcccaaac caatccaata caacgacccg gtacctgacc acgagtcctt
     1681 tacccttgct tcaaagatac tgaacgaaac acgtacactc aacgtttggc tgccaccaca
     1741 gtacaagagt ggcgatgcaa agtatccggt cgtatatatg ccagatggtg gtattgtggg
     1801 tgaagatttt ccgcatatag ccaatacttt ggctgagctc atctccgcag gtacgataca
     1861 gcctgtcata ttggtgggca tacagaatac caaccgtaag cgcgacctta ccgggcctac
     1921 ggtggtagcc aaagataagg aatggatacc cgaaaacggt ggttctgatg cattcagacg
     1981 attcatccgc gacgagctat tcatggaggt aaacaagcgt taccgcacca cagatacacg
     2041 taccatcgtt ggcgaatcgc tggcaggctt gttcgtaacc gagacatttc tgcttgctcc
     2101 cgacatgttc aacaattaca tagctattga tccatcactc tggtggaatg accagtatct
     2161 tgtaaagaac gccggccgac ttcttgccac aggcgatatg aatggcagaa aattctggtt
     2221 tgcaggatct tcgactaagg atattcaaga agctacctcc aagctcgccg atgcacttac
     2281 taaagtagtg cgccccggtc tgcactggaa atattcgccc gaaccaaacg aaaaacacaa
     2341 caccatcttc agggctgtca aacaaaaggc tttcctttgg atgcttggga aataacacac
     2401 aacattcccg acaaatcttg ataaaacttt cacattttgt tgcataacac atcgaaataa
     2461 tgtaatttta gcgcagcaaa gaggtgtttc ttttttgcgt ttaggtatca ttcaagcatc
     2521 aaacattcaa gccatgccaa gaattgcatt atttcttgct gttatttgct tattcggcaa
     2581 tattgcccgt gcacagcaaa gctcacagac acaacaactc atcctccaac tcaagaacga
     2641 agtagccgac aacaaccaac agcagggcga aataagttct atcggacatc cggtacttga
     2701 tcaactggca cgtgattatc aggttttgaa gataaatatt taccgcgcag gtatccatac
     2761 cggaaaatat ttctacacac tatcgtttcc aacagataca gatatggata ggctcgccga
     2821 aatattcgta cacacaggtg aggtaaggta tgccgaaccg gattatacag gtacgtcggg
     2881 cggtcaactc ggcactgtac ccaatgatac ctaccacaac cgacagtggg gcttgcacaa
     2941 tacaggcaat ttttctcttg caccttccaa agcaggagca gatatcgaaa tgcataatgc
     3001 ttgggacata agccagggta gcagcgatgt ggtagtagcc attatggaca gtggtatcaa
     3061 attggaccac cccgaattat cgggccgtat ctggcaaaat accgaggaga tcgccggcaa
     3121 cggactcgat gacgataaca acggctatgc agacgatacc caaggctggg attttgtcaa
     3181 caacgacaac actcctgctg atgacctcgg gcacggcacc aacattgcag gcatcatcgg
     3241 tgccaacggc aataacagta taggttacgc cggcatcgac tggcattgtc ggctcatggc
     3301 tctcaaaggc ctcaatagtc agaacagcgg ccagtattca tggtggtcat cggcgttata
     3361 ttatgcagca gacaacggag cccatatcat caacctttca gtaggtggct ccggtttttc
     3421 gcagacactc aaagatgcag tcgattattg cctcgacaaa ggtgtatgcc tcgtagtctg
     3481 tatgatgaac accaataccg agacgcctta ctatccggcc gcatttcccg gcgtcatcgc
     3541 agtaggttct accaaccccg acgatacccg cactcaacct ttcttctggg atgcagccag
     3601 tggcagcaac tacggcagcc acatatcagt tatagcaccc ggcaactaca cttacggtct
     3661 gcaccacttg tccaacacca attataacac atactggggt ggcacatcac aagccacacc
     3721 acacgtagta ggcgttgctg ccttgctgct ggcacaggac cctacacgta cacctgccga
     3781 catcaagaac atcatc
//
LOCUS       DAOQQY010000276         3795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_11718_length_3795_cov_4.388503, whole genome shotgun sequence.
ACCESSION   DAOQQY010000276 DAOQQY010000000
VERSION     DAOQQY010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11718_length_3795_cov_4.388503"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3064)
                     /locus_tag="PLE23_11060"
     CDS             complement(<1..3064)
                     /locus_tag="PLE23_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMX83847.1"
                     /translation="MFNRFIHRPVLSIVISLIIALLGILAVTQLPITQYPSISPPKVN
                     ITADYPGANGELMVKAVIIPLERAINGIPGMKYMTSDAGNDGEAGIQVVFNVGTDPNI
                     ASVNVQNRVAAVINKLPPVVVREGIKITREESNMLLYINLFSNDTSLDDQFLFNFADI
                     NLLPELKRVNGVGFVDILGNREYAMRIWLKPDRMLAYNTSAEEVLKAINEQSLEASPG
                     RIGESSGKKSEAFEYVLKYPGRYTTKEEYDNIIIRADNNGQLLRVKDVADVEFGSSYY
                     DLYSRLNGKTSAAIVIKQSYGSNASTTIENVKKKMEEIKAESFPKGLNYEISYDVSTF
                     LDASIEKVIHTLVEAFILVGLVVLLFLGDWRSTLIPVIAVPVSLIGTFFFMQFFGITI
                     NLITLFALVLAIGIVVDNAIVVVEAVHAKMEESHIAPMEATEQAMQEINGAIIAITLV
                     MAAVFIPIAFMSGPVGIFYRQFSITMATSIILSGVVALTLTPALCAILLKNKHNDVPS
                     KNIFTKLLHGFNNWFDSLSGKYTNLLKRLVNRQLVTFVTLILFILGTWGIGSVLPSGF
                     IPNEDQGLFYAIILTPPGSTLERTNEVSTQLQNIASSIPDIKSVSSTAGYEILSEGTG
                     ANEGTCLVNLKNWSERKHSLKEVIRILEEKIKDIKGAQIEFFPPPAVPGYGAAGGFEL
                     RLLDKTGSGDYKKMETVNRDFVAALNKRRELSSVFSFYNASYPQYMVKIDNEKALQKG
                     VTIENATNTLSTLIGSDYETSFIKYDHQYKVMVQALPSYRALPEDLLKLYVKNDKDEM
                     VPFAAFMTIEKVYGLSEITRHNMYNSSEISGGTAQGYSSGAAIKAITEVAQNTLPKGF
                     GIEWAGISRDEVDQGNQAIYIFLICLIFVYLILAAQYESFVLPLPVILSLPTGIFGAF
                     LFLKLLGLENNIYAQIAVIMLIGLLGKNAVLIVEFAVQRHAAGKSAFKSAVEGASVRL
                     RPILMTSFAFIAGLLPLVVATGPGAVGNRTIGSTALGGMLFGTLFGIFI"
     gene            complement(3098..>3795)
                     /locus_tag="PLE23_11065"
     CDS             complement(3098..>3795)
                     /locus_tag="PLE23_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMX83848.1"
                     /translation="ISKAKLDMAKAELSEAELYLGFTKIKAPFDGCIDRLKFKKGSLI
                     DEGTLLTTLSNNRDVYAYFNVSESEYLDYKKQISQDKQGVSLILANGETHKYKGRIET
                     IEGEFDNSTGNISFRAKFPNPDNLLKHGETGKVVVTVPVNKALLIPQKSTFEIQDKIY
                     AYVMQHDNKLKAVNITVRQAIPNLYILNSGISPNDKILLDGVQNVKDGDKIQPVYVNP
                     TGVMAQLQLIKQG"
BASE COUNT          991 a          781 c          879 g         1144 t
ORIGIN      
        1 taatgaagat gccaaagaga gtaccgaaca gcataccgcc aagtgcagtg gacccgatgg
       61 ttctgttgcc aactgcaccc gggcctgtgg ctaccaccag tggcaggagc ccggcgatga
      121 aggcaaagga agtcatcaat attggtctta aacgaacaga ggcgccctct acagccgatt
      181 tgaaggccga tttgccggca gcgtgccttt gaactgcaaa ttcaacaatg agtactgcat
      241 ttttgcctaa aagcccgata agcatgatga cagctatctg tgcatatatg ttgttttcga
      301 gaccgagaag ttttaagaac aaaaatgctc cgaatatacc tgttggtaat gagaggataa
      361 caggtagagg tagtacaaag ctttcgtact gtgctgctag tatgaggtac acgaatatca
      421 gacagatgag gaatatgtat atagcctggt tgccctggtc aacttcatca cgtgatatgc
      481 ccgcccattc aataccaaag cctttgggaa gagtgttttg agctacctcg gtgatagcct
      541 tgatagcggc accactacta tatccttgag ctgtaccacc gcttatttcg gatgaattgt
      601 acatattatg tcttgttatt tcagaaagac cgtaaacttt ttctatggtc atgaatgcgg
      661 cgaacggcac catttcatcc ttatcgtttt tgacatagag cttcaggagg tcttcgggta
      721 gtgccctgta cgaaggcagt gcctgcacca ttactttgta ctggtggtcg tatttgatga
      781 agctggtttc gtaatcgcta ccgatgagtg tagagagggt attggtggcg ttttcgatag
      841 tgacaccttt ttgcagagct ttttcattat caatcttgac catgtactgc gggtagcttg
      901 cattgtaaaa actgaacaca gatgacagtt cgcgtctttt attgagtgca gcaacgaaat
      961 cacggtttac ggtttccatt ttcttgtaat ctcctgaacc ggtcttgtct agcagcctga
     1021 gctcaaaccc acctgccgct ccgtagcctg gtactgcagg aggcgggaaa aattctattt
     1081 gagcaccttt aatgtctttt attttttctt ccaaaatgcg gattacctct ttgagagagt
     1141 gttttctttc ggaccagttt tttagattga ccaagcaggt gccttcgtta gctccggtac
     1201 cttctgacaa tatttcataa ccggctgtcg atgaaaccga cttgatatcg ggtatggaag
     1261 atgcaatatt ctgtagctga gtacttactt cgttggtgcg ctcgagggta gagcccggag
     1321 gtgttagaat gatggcatag aacaaaccct ggtcttcgtt agggataaat ccggacggca
     1381 aaacagaacc tataccccat gtgccaagaa taaatagtat caaagtgacg aaagtcacaa
     1441 gttgtcggtt gaccagtctt ttgagcaggt tggtgtattt gccgcttagt gaatcgaacc
     1501 agttgttgaa cccatgtagc aatttggtaa agatgttttt ggagggtaca tcattatgtt
     1561 tgtttttgag caggatggca cataatgccg gagtaagcgt aagtgctaca acgcccgaca
     1621 gaatgatgga tgtagccatg gttatagaaa actgcctgta gaaaatgcca accggccccg
     1681 acatgaaggc aataggtata aatactgcag ccataaccaa agtaatggca ataatggcac
     1741 cgttgatctc ctgcatggcc tgttcggtgg cttccatcgg tgcaatatgc gattcttcca
     1801 tctttgcgtg tactgcttct acgacaacaa tggcattatc cactacgata ccgatggcca
     1861 gcacaagagc gaataatgta ataaggttga tggtaatacc gaaaaactgc atgaaaaaga
     1921 aagtacctat taacgaaacc ggtacagcaa taaccgggat gagtgtggaa cgccagtcgc
     1981 caaggaatag caatacgact agacctacca gaataaatgc ttcaaccaga gtatgtatga
     2041 ccttctcaat ggaagcatca agaaatgtgg atacatcata gctgatttcg tagttgagac
     2101 ctttaggaaa tgattcagcc ttaatttcct ccattttttt cttgacgttt tcgatggtag
     2161 tacttgcatt gctgccgtac gactgtttga ttacgatggc agccgaggtt ttgccattga
     2221 ggcgtgagta gaggtcgtag tacgagctac cgaattctac atctgccaca tcctttacgc
     2281 ggagcagctg tccgttattg tctgccctga tgatgatgtt atcgtactct tcctttgtag
     2341 tgtatcttcc cgggtatttc agtacatatt cgaatgcttc cgattttttt ccggaacttt
     2401 caccgattcg tcctggtgag gcttcaaggc tttgttcgtt gatggccttt agcacttctt
     2461 cagctgaggt attataggca agcatgcggt cgggttttag ccatattcgc atggcatact
     2521 cccggttgcc aaggatatct acaaacccga caccgttgac ccgtttgagc tcaggcaaaa
     2581 ggttgatatc ggcaaaattg aataaaaact gatcatcaag cgaagtgtca ttgctgaaca
     2641 agttgatata taagagcatg ttcgactctt cgcgtgttat tttgatgccc tcacgtacta
     2701 ccacaggtgg cagtttattg atgacagctg ctacccgatt ttgtacattg acagaagcaa
     2761 tgttagggtc agtaccgaca ttgaatacca cctgaatacc ggcttcaccg tcattacctg
     2821 catctgaagt catatacttc atgcctggga taccattgat agcccgctca aggggtatga
     2881 tgacggcctt aaccatgagc tcaccattgg cacccgggta gtctgcggta atattgacct
     2941 ttggcggtga gatggatgga tattgtgtga tcggtagctg ggttactgcc agaataccta
     3001 gtaacgcaat gataagtgat atcacgatgg atagcaccgg ccgatggatg aatctgttga
     3061 acataaatat tttttactaa gctgtattta gctttgatta tccttgtttg attaattgta
     3121 attgtgccat aacacctgtg ggatttacat acactggctg tattttgtcg ccatccttga
     3181 cgttctgtac tccatctaac agtatcttat cgttgggtga aatacctgaa tttaaaatat
     3241 aaaggttagg tattgcctgc ctgacggtaa tatttacggc cttcaactta ttatcatgtt
     3301 gcattacata agcgtagatt ttgtcttgga tctcaaaagt agatttttga ggaatgagca
     3361 aagctttgtt gacgggcact gttactacta ctttgcctgt ttctccatgt ttgagcagat
     3421 tgtcggggtt tggaaatttg gcacggaaag atatattgcc agtactgttg tcaaattcgc
     3481 cttcaatagt ttctatgcga cctttgtatt tgtgtgtttc accattggcc aatattaggc
     3541 ttacaccttg tttgtcttga gaaatttgtt ttttgtaatc gagatattct gattcagata
     3601 cattgaaata tgcatataca tcgcggttat tggagagtgt ggtaagcagt gtgccttcat
     3661 ctatgagact tcctttctta aatttcagtc tgtcaataca accatcaaaa ggcgccttta
     3721 ttttggtgaa tcctaggtat agctcggctt cagaaagctc agcttttgcc atgtcaagtt
     3781 tggcctttga gattg
//
LOCUS       DAOQQY010000277         3717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_12100_length_3717_cov_5.780721, whole genome shotgun sequence.
ACCESSION   DAOQQY010000277 DAOQQY010000000
VERSION     DAOQQY010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12100_length_3717_cov_5.780721"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="PLE23_11070"
     CDS             complement(<1..1364)
                     /locus_tag="PLE23_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase"
                     /protein_id="HMX83849.1"
                     /translation="MKKQLFGLFAALVVLIGFNACKPKNTMTAVSGDAAAKAYVAPGK
                     FDEYYNFVSGGFSGQMSVYGLPSGRLLRVIPVFSVDPEKGWGYSEETKPMLNTSHGFV
                     PWDDLHHVELSQTNGEINGKWVFGNANNTPRVARIDLKTFKTAEILELPNSAGNHSSP
                     FGTENTEYIVAGTRFSVPGDNDNGDVPINSYKKNFKGHISFISVDQNKGDMNLAFQIK
                     TPGINFDLARCGRAKSHGWFFFSCYNTEQANTLLEVNASQKDKDFIMAVNWKKAEEYL
                     KAGKGEKQKVRYAHNKYDEATHTSKSEIKTDVIVLDAAKLKDICYFIPCPKSPHGCDV
                     DPTGEYIVGSGKLAALLPVFSFDKIQKAIADKAFDGEYDGIPVIKYDAALYGEVKKPG
                     LGPLHTEFDGKGNAYTSFFVSSEVVKWNIKDLKVVDRQPTFYSVGHLCIPGGNTRKPS
                     PKYL"
     gene            complement(1391..1888)
                     /locus_tag="PLE23_11075"
     CDS             complement(1391..1888)
                     /locus_tag="PLE23_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMX83850.1"
                     /translation="MKKVPFFVLILFLFAACGGSDTETTEAEGTAGSEETTAEAPKSM
                     VGDVSQYDPHRGEGKFTSVDVGDKLDAAMATEGEKVQGVKCASCHKLSDERLVGPGWK
                     GLTSRKKPEWIMNFITNPDAMIDKDPELQAQLEICLVRMPNQNLTDNEARSIYEFMRK
                     NDGVK"
     gene            2124..2690
                     /locus_tag="PLE23_11080"
     CDS             2124..2690
                     /locus_tag="PLE23_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83851.1"
                     /translation="MKANNIIVTFLYIIVGLMSTACNTDKNTSVPTTTPSPKMSTADS
                     LDNLVGVVRTTELINYISLPDGYFLKNTVNLADEYEGFLFENQNEFDNVIGFGKTANN
                     APIHINFDENVVVGIAGKAKAEQVRIKPQTILKRENEIVFKFSADKPQTQSFQSTPLF
                     LLYIPKSALHNIRKMQLNGKDIKLNDQE"
     gene            2861..>3717
                     /locus_tag="PLE23_11085"
     CDS             2861..>3717
                     /locus_tag="PLE23_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HMX83852.1"
                     /translation="MQFLYPAFLFGLLALAVPVIVHLFYFRKFKTIYFTNVRFLKELK
                     QETSARQKLKNLLVLLMRALAIAAMVFAFAQPFIPKGNVIKHGLKSVSIFIDNSYSMN
                     ATDKDLSLFEKAKFKAKELINGYKEEDNFQVLTNDFEGKHQRLMSKEDALAAIDELKT
                     SPAVRNISDIMKRQQQVLNTSDRSLKIAYILSDFQKNISDFSNKPDSLIEYNLIQLRA
                     ITESNVSIDSCWFEIPVQMANQVNPLVVKVHNYSDEPAENVTLTLNYDGQQKPVGNLN
                     IAANASITDT"
BASE COUNT         1125 a          770 c          668 g         1154 t
ORIGIN      
        1 atcagatact taggtgatgg cttgcgtgta ttaccacctg ggatgcaaag gtgacctaca
       61 gaatagaatg taggctgtct gtccactacc ttaaggtctt tgatattcca cttcactact
      121 tcagatgaaa cgaaaaatga agtataggca ttaccttttc catcaaactc agtatggaga
      181 ggacctagac ccggcttctt cacttcaccg tatagagcag cgtcgtattt tattaccggt
      241 atgccatcat attctccatc aaaagctttg tcggcaatgg ccttctgaat cttgtcaaaa
      301 ctgaataccg gaagcaatgc agcaagttta ccactaccta ctatgtattc acctgttggg
      361 tcaacatcac atccgtgagg tgacttaggg caaggaataa aatagcagat atctttcagc
      421 ttggcagcat ccaatactat tacatcagtc ttgatttcag attttgaagt gtgtgtagct
      481 tcatcatatt tattatgagc atatcttact ttttgctttt cgccttttcc tgctttcaga
      541 tattcttcag ctttcttcca gttgaccgcc atgatgaagt ctttgtcttt ctgagacgca
      601 tttacttcaa gaagcgtatt ggcctgctct gtattataac agctaaagaa gaaccaaccg
      661 tgtgattttg cacgtccaca acgagcaagg tcaaaattaa tgcccggagt cttaatctgg
      721 aatgcaaggt tcatgtcgcc tttattctga tcaacactga taaaactgat gtgtccttta
      781 aaattctttt tgtaagaatt gattggaaca tcaccgttat cattatcgcc cggcacgctg
      841 aatcgtgtac ctgctacgat atattcggtg ttttcagtac caaaaggtga actgtggtta
      901 ccggcactgt ttggcagttc cagtatttct gcagttttaa aagtttttag gtcgattctg
      961 gctacccttg gtgtattgtt ggcattacca aacacccact ttccgttgat ttcaccatta
     1021 gtctgtgata actctacgtg gtgaaggtcg tcccaaggca caaagccatg agaagtgttc
     1081 aacataggtt tagtttcttc actataaccc caaccctttt ccgggtcaac tgagaataca
     1141 ggaataacgc gtaacaaacg tccacttggg agcccgtaaa cactcatttg tccgctgaaa
     1201 ccaccactca caaagttgta gtattcatca aacttaccgg gtgccacata ggctttagca
     1261 gccgcatctc cacttaccgc tgtcatcgtg tttttaggct tacatgcatt aaaccctatc
     1321 aaaactacca gggcagcaaa aagaccaaat aattgctttt tcataaatat gtgattttaa
     1381 ttagttttgt ttacttaaca ccgtcgttct ttctcataaa ctcgtaaata gaccgtgctt
     1441 cattgtccgt aaggttttgg tttggcattc ttacgaggca gatttcaagt tgtgcctgaa
     1501 gttctgggtc tttgtcaatc atagcgtccg gattggtgat gaagttcata atccattcag
     1561 gttttttcct tgatgtcaaa cccttccatc ccggacctac aagcctctcg tctgaaagtt
     1621 tgtgacatga tgcacattta acaccctgta ctttttcacc ttctgttgcc atggcagcat
     1681 caagcttgtc accgacatct acacttgtaa atttaccttc acctctgtgt gggtcgtatt
     1741 gactgacatc tcctaccata ctcttaggtg cttctgctgt tgtctcttca gatccggcag
     1801 taccttctgc ttctgtggtt tcagtatcag aacctccgca agcggcaaaa aggaatagaa
     1861 tcaaaacaaa aaatggaact tttttcatag ctttaaaaaa ttttgagtta taaatacgat
     1921 acaaatttcc aataaatcag aacaatcatt ttatgacttt aatcatattg ctcacgtgat
     1981 tcttctcact tgtatgctaa agactacata atgtattgaa gaaagggcaa atataagatt
     2041 tgcaaacaat tttatcacta caattacata cgctcttgca tttatcgcta aagtaactca
     2101 gttttgtgaa aaattttatc aaaatgaaag ctaataacat cattgttacc tttttataca
     2161 ttattgttgg ccttatgtca actgcctgca atacagacaa aaacacaagt gtgccaacca
     2221 ccaccccaag ccctaagatg agtacagctg attcgcttga taatcttgta ggtgtggtac
     2281 gaacgaccga attaatcaat tatatttctt tgcccgacgg atattttctg aaaaatacag
     2341 taaatctggc tgacgaatat gaaggatttc tgtttgagaa tcaaaatgaa tttgacaatg
     2401 tgataggatt cggtaaaaca gcgaataatg cacccattca tattaatttt gatgaaaatg
     2461 ttgttgtcgg tatagccgga aaagcaaaag cagagcaggt aaggattaaa ccacagacca
     2521 ttctcaagcg tgaaaatgag atagttttta aattctccgc tgataaacct caaactcaat
     2581 cttttcaaag tacaccgctt tttttgttgt acataccgaa gtctgcctta cataatattc
     2641 gcaaaatgca actcaacgga aaagacatta agctaaatga ccaagagtaa ttaaaatccg
     2701 aaagtaaaat aatcgaagac gattaagtct atattttcca attagcattt gtgggcttaa
     2761 gcatttaaaa atttcctttt tgtgtagtga gctcatgaac aaagggcaat aatattacgt
     2821 attagtagtt tgtaataaat caaatttcag tcagagcaat atgcagtttt tatatccggc
     2881 gttcttattt ggtcttttgg cgttggcggt gccggtgatt gtccacttat tctatttcag
     2941 aaaattcaaa accatttatt ttaccaatgt tagatttctg aaagaattaa agcaggaaac
     3001 atcagcaagg caaaaactga aaaatctgct tgtgctactg atgcgggcac tcgctattgc
     3061 cgctatggtg tttgcctttg cccaaccatt tattcctaaa ggaaatgtca tcaaacacgg
     3121 acttaaatcg gtaagtatat ttattgacaa ctcatactcc atgaatgcca ccgataagga
     3181 tttgtcattg ttcgaaaaag ccaaatttaa agcaaaagaa ctcatcaacg gttataagga
     3241 agaagataat tttcaggtac tgaccaatga ttttgaaggt aagcatcaac gcctgatgtc
     3301 aaaagaagat gcactcgccg ccattgatga gttaaaaaca tcccctgccg ttcgtaatat
     3361 ttcagacata atgaaacgtc aacagcaagt gctcaatact tccgaccgta gtcttaaaat
     3421 agcttatatc ctgtctgatt ttcaaaaaaa tatttctgat ttttcaaaca aacctgattc
     3481 gctcattgaa tacaacctga tacaactcag ggctattaca gaatctaatg tttcgatcga
     3541 cagctgttgg tttgaaattc cggttcagat ggccaaccag gttaatccac ttgtagtaaa
     3601 ggtacataat tatagcgatg aacctgctga gaatgtgaca cttacgctca attatgacgg
     3661 ccaacaaaaa ccggtaggta acttgaatat tgccgccaat gcaagcatta cagatac
//
LOCUS       DAOQQY010000278         3686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_12252_length_3686_cov_5.123933, whole genome shotgun sequence.
ACCESSION   DAOQQY010000278 DAOQQY010000000
VERSION     DAOQQY010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3686
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12252_length_3686_cov_5.123933"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..157
                     /locus_tag="PLE23_11090"
     tRNA            72..157
                     /locus_tag="PLE23_11090"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:106..108,aa:Leu,seq:taa)
     gene            complement(722..1687)
                     /locus_tag="PLE23_11095"
     CDS             complement(722..1687)
                     /locus_tag="PLE23_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83853.1"
                     /translation="MKSTISLLILFFLCCMGCQEKADSPFVLPWEQVKQKGIGAHLNI
                     IVEQTDEQDITILKRMFDEVSDSFKINIRYRLKPANKIADILLADSKLDKKSKYDLVI
                     ASDEVMSELNTLGLLYANYHSSIPNFSNLNLRAKQILTKRNQHGLFLVPVTMRCPGVV
                     YDTTVHQNIPIPGVDMYPYIYKCNSAFNLSLLVAKRIGQNRMPALDSIDYLSLLKGKS
                     DRDSLTSYLKQYILPIDSIKPGMNKLVLMSPFNFDLLKSKGSLNIYENADIPCMNTCA
                     GILNSSNSVFGAMLLINNFLDENTQHINYEMSHHYFRPVVRINNI"
     gene            2036..>3686
                     /locus_tag="PLE23_11100"
     CDS             2036..>3686
                     /locus_tag="PLE23_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HMX83854.1"
                     /translation="MRTRINYWVLRASLAVLLCCGIFFSTKTNAQCGYSYQNAPFVLT
                     VDMFSSTTTINQGTLGPILGPTAGCTLYFEEPLGTLTATYTLDCTMGPGPYTITVRSD
                     DDGVIDGSTSAPVSVTVNLVDKVTPLIICPNNVVGTTDADGTGDCLLHWNTGGISSIA
                     LTNVFPVAPANPGEYSENCSHTVEFNVNGGAWIAGNDAGDYSYSPGVNTVTYRITDMS
                     GNTVSCSFTVTVTDNEAPIWNNSLINGLGSELAQDFTVPPTLRRIVLDCNHANYAADL
                     AYLLNTWLPSATDNCDPTVTIARNYLGSNPVACATRLGITNVYNRDRIQYTATDAAGN
                     VSILFTLHILTADNKGPNYANGTVPSGPAVEGLPNQYNGPKLTVKISDYYDPTDPATP
                     CGIDFTNAAPVINPTPSLVATPTDCQAINYFGWAVTPLGSSPAPSTLFLIGSNNPAQF
                     YGVGNYNLGYVSYDPCLNFASYTFELEVIDDVPPIITNCPANIANVPNEAGNCAATVS
                     WSKPVVTDNCSAILLPNKSEFQDPNGNIFPIVIGGGADDYGV"
BASE COUNT         1104 a          728 c          678 g         1176 t
ORIGIN      
        1 tacaaatata cttttacttt tgcacagcct ttacagcatg aaaatgtcat aggatatttt
       61 cttaaattat tgcccggatg gtgaaattgg tagacacgca ggacttaaaa tcctgtgact
      121 agtgatggtc gtgtgggttc aagtcccact ccgggtactt cttctacaac aataatgttc
      181 agtcagcgat gtctcagtac aatttcctga attgtgttag atgaacttag attgcgcata
      241 aggctcttta ctgcaaataa ttattacttc taatccatgg caaaagctca aatccatagt
      301 tcactatgct ttcatctctt ttttatggat tttttgtaag aatcattttc gtatatattt
      361 gtaatgcata atccgggttt aataaatcta ctacttaatg gttaaaaaaa tgccgaaaaa
      421 taacgtgtca caacatcaca cgtctcacat ctcactttta atttttaatt ttttaatttt
      481 taatttttaa ttgaatgcgc gtgaaggata gaagccggca caacgtaagg cggatagcct
      541 gacccgacag gggcacgccc tgattaaacc ccgatgacgc attatcttct ttggagcaaa
      601 taatgattcg ttccaatcca tttcaaaagc tcaaaaccaa agtttactat gtttttatct
      661 cttttttatg gattttttgt gctaatcatt ttcattaaaa tacctaatgc gaaacctggg
      721 attaaatatt attaatccgc acaaccggcc gaaaataatg gtgtgacatt tcgtagttaa
      781 tgtgttgtgt attttcgtca agaaaattat tgattaataa cattgctcca aaaacactat
      841 tcgacgaatt taaaatgccg gcacatgtat tcatgcaagg tatatccgca ttttcataaa
      901 tattgagact acccttagat tttaataaat caaaattgaa tggagacatc agtaccaact
      961 tgttcatacc gggttttata ctatcaatcg ggagtatgta ttgtttcagg tagctagtaa
     1021 gactgtctcg gtcagacttt cctttaagca gcgagaggta gtcaatacta tctaaagcag
     1081 gcattctgtt ttgccctatt cttttagcta ccagcaaact caagttaaat gcactattac
     1141 acttatatat atatggatac atatcaacgc ctggtatggg aatattttgg tgcacagtag
     1201 tatcatatac cacccccggg cacctcatag taaccggaac caaaaacaat ccgtgttggt
     1261 ttcttttggt taatatttgc ttggctcgaa gattgagatt tgaaaaattt ggaatggaag
     1321 aatggtaatt agcatacaat aggccaagtg tatttaattc ggacatgact tcatcagaag
     1381 cgatcacgag gtcatattta gattttttat cgagcttcga atctgccagg agtatgtctg
     1441 caattttatt tgcaggtttt aatctatatc taatattaat tttaaatgag tcactgactt
     1501 catcgaacat tcttttcagt atcgttatat cctgttcatc cgtttgctca acaataatat
     1561 taagatgagc tccaataccc ttttgtttaa cttgctccca cggaagaaca aagggagaat
     1621 cagccttttc ctggcaaccc atgcagcaca aaaaaaacag gatgagcagg ctgattgtag
     1681 atttcattat gcaatattag ggattcaatc gttaaaattt gaatttatta tcatgaaaaa
     1741 tgaaaaaact attgacactt aatataaaat ctctttattt tgtacattac ttttacctgc
     1801 ctctctacat aaagctttcg cattatcccc ggcgaccgtt gttttagaaa aattttaaaa
     1861 aaaaaatcat gaattgctac caatttggtc aaaattgtta cttttgccct attgattgtt
     1921 ttaatttagc aatcagtgga gttattttta agagaaaacc aaaccaaaaa tcgaacaaat
     1981 gcagctaaaa atctccccga acagttatta tttcttaata aaaccttcca ttttcatgag
     2041 aacgcggatt aattattggg tgctacgtgc atccctggca gtgctgttat gttgtggtat
     2101 cttttttagt accaagacga atgcacaatg tggatacagc tatcaaaacg ctccgtttgt
     2161 gttaactgtg gacatgttca gttcaactac caccatcaat cagggaacat taggcccgat
     2221 tttaggtcct acagcaggtt gtacattgta tttcgaagag cctttaggta ctctgacagc
     2281 tacctatacc cttgactgta caatggggcc cggcccgtac accataacgg ttcgttcaga
     2341 tgatgacgga gttattgacg gttcaacaag tgcaccagtt agtgttactg ttaatttggt
     2401 ggacaaggtt acacccctta taatttgccc gaacaatgta gtgggtacaa ccgatgctga
     2461 tggtacaggt gactgtctgc tgcattggaa taccggtggt atatcttcta ttgcacttac
     2521 caatgtgttt cctgtagctc ctgccaatcc gggagaatac agcgaaaatt gttcgcatac
     2581 cgtcgaattc aatgttaatg gcggagcatg gatagcaggt aatgacgccg gagattatag
     2641 ctattcgcct ggtgtaaata ccgtaacgta tcgtattacg gatatgtccg gcaatacagt
     2701 cagttgttca tttactgtga ctgttacaga taacgaagca ccaatctgga acaacagcct
     2761 tattaacgga ctcggttctg aacttgctca ggactttact gttcctccaa cactcagaag
     2821 gattgtactt gactgtaatc atgcaaacta tgctgccgat cttgcatatc ttttgaatac
     2881 atggttgcca agtgctacag ataactgtga cccaactgtg accattgcaa gaaattatct
     2941 tggttcaaat ccggtagctt gtgctactcg tctcggtatt acaaacgtat ataacagaga
     3001 cagaatccaa tatacagcaa cagatgctgc aggtaatgtt tctattttat ttacattaca
     3061 catattgacg gccgacaaca aaggtcctaa ttatgccaac ggtacagtac cttccggccc
     3121 ggcagtagaa ggactaccaa atcaatataa cggacctaag cttacggtaa aaatatctga
     3181 ttattacgac cctacagatc cggctacacc atgtggtatt gactttacca atgcggcacc
     3241 ggttatcaac ccgacacctt cactggtagc aacacctact gattgtcagg ctataaatta
     3301 ttttggatgg gctgtgacac cacttggaag ttcacctgct ccatcaacac ttttcttaat
     3361 tggcagtaat aatccggcac agttttatgg tgttggtaat tacaatttgg gttatgtatc
     3421 ttatgaccca tgccttaatt ttgcatcata cacttttgag ctagaagtta tagatgatgt
     3481 tccgccaatt ataacaaatt gccctgccaa tattgctaat gtaccgaatg aagcaggtaa
     3541 ttgtgctgct acagtatctt ggtcaaaacc ggtagttaca gataattgtt ctgccatttt
     3601 actccctaac aagagtgaat tccaggatcc taacggtaac attttcccaa ttgtaatagg
     3661 aggtggtgct gacgactatg gtgtat
//
LOCUS       DAOQQY010000279         3651 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_12428_length_3651_cov_5.702447, whole genome shotgun sequence.
ACCESSION   DAOQQY010000279 DAOQQY010000000
VERSION     DAOQQY010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12428_length_3651_cov_5.702447"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /gene="ruvB"
                     /locus_tag="PLE23_11105"
     CDS             complement(<1..637)
                     /gene="ruvB"
                     /locus_tag="PLE23_11105"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMX83855.1"
                     /translation="MRNQNIDPDKDRMPSEEVQIEKSLRPAALEDFNGQPKIVDNLKV
                     FIQAALARGEALDHVLLHGPPGLGKTTLSHIISNELGASLKMTSGPVLEKPGDLAGLL
                     TNLSEGDVLFIDEIHRLNTVVEEYLYSAMEDYRIDIMIDSGPNARSIQISLNPFTLIG
                     ATTRMGLLTAPMRARFGINCLLDYYNVETLEKIIYRSAALLNIPITKEGALE"
     gene            complement(651..1238)
                     /locus_tag="PLE23_11110"
     CDS             complement(651..1238)
                     /locus_tag="PLE23_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NigD-like N-terminal domain-containing protein"
                     /protein_id="HMX83856.1"
                     /translation="MIEKILLSCTFLLILSCNRTVVTNSGSQEETRATVKDMRDLDGC
                     TYFLITDDGKKLLPNDLTVIPFSLMDGQRVLFTYKKAQNVAGICMAEDMIIDILSICI
                     ADKTTKPSKLQCIDMIDPVKTEWGRRLMNNQRVNKMWKFTYQDGFAYYFESQEKNLLY
                     DCQGNLMCTDEPAKHNCIDNIAELTNGKIVWVLNH"
     gene            1340..2668
                     /gene="icd"
                     /locus_tag="PLE23_11115"
     CDS             1340..2668
                     /gene="icd"
                     /locus_tag="PLE23_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMX83857.1"
                     /translation="MSGSKITIFRGKLKVPDNPIIPFIEGDGTGRDIWRASVRVIDAA
                     VEKAYKGKKKIEWKEVLAGEKAFKQTGNWLPDETLDIIREHLVAIKGPLTTPVGGGIT
                     SLNVALRQILDLYVCLRPVQYFTGVPSPVKHPEKVDMVIFRENTEDIYAGIEFTGGSE
                     DSDKILNFIKKNYPKFFSKIRFGTSAAVKSWNKAIKSKEAATVEVGIGIKPVSRVGTE
                     RLVKAAIDYAIANNRKSITIVHKGNIMKFTEGGFRDWGYKYAETHYSNQTYTWAQWER
                     TKKDKGEEAANKEQADALASGKILIKDAIADIALQQVLTRPDDFDVIATLNLNGDYLS
                     DALAAQVGGIGIAPGANINYVTGHAIFEATHGTAPKYADQDKVNPGSVILSAEMMLRY
                     MGWTKAADLILKGLNGAISSKNVTYDFHRLMEGAKLVKCSEFGDEIIKKM"
     gene            2889..>3651
                     /locus_tag="PLE23_11120"
     CDS             2889..>3651
                     /locus_tag="PLE23_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMX83858.1"
                     /translation="MEGFEVLQAGDIKAGLKKLETALIDVVLCDVKLPDGSGIDATRI
                     IKEKYPNIEIILLTAYGNIPDGVQAMKNGAFDYITKGDDNNKIIPLLYKAVEKVTLAN
                     RVTLLEKQLEHKYSFESIVGKSKAILSSIESARKVSTSEATVLLTGETGTGKEVFAQA
                     IHHSSLRSKHNFIAVNCSAFSKELLENELFGHKAGAFTGATKDTKGIFEEANNGTVFL
                     DELGEMPLDLQVKLLRVLETGEYLKVGDSKPTKVNV"
BASE COUNT         1155 a          726 c          787 g          983 t
ORIGIN      
        1 tttcaagggc gccttctttt gttatgggta tattgagcaa tgcagcagag cggtagatga
       61 ttttttcaag tgtttcgaca ttataatagt caagtaggca gttgatacca aatcgtgccc
      121 gcataggtgc agtgagtaac cccatacggg tggtagcacc tattaaggta aacgggttga
      181 gcgatatctg aatactacgg gcattgggac cgctatctat catgatgtct atgcggtaat
      241 cttccatcgc cgaataaaga tattcctcta caactgtatt caaacggtgt atttcatcga
      301 taaacaaaac atctccttca gacagattag taagcaggcc ggcaagatca ccaggctttt
      361 cgagtactgg ccctgaagtc atcttgagtg aggcgcccag ttcattgctg ataatgtgcg
      421 acagggtggt tttgccaagc cccggcggac catgaagcaa tacatgatcc agtgcctcgc
      481 cccgtgctaa agccgcttgt atgaacactt tcagattgtc aactatcttg ggttgcccat
      541 tgaagtcttc cagggcggcc gggcggagcg acttttctat ctgtacctcc tctgatggca
      601 ttctgtcctt atccgggtca atattttgat tgcgcatatt aaaaagataa ttaatgattg
      661 agaacccaca ctattttgcc attggtgagt tcggcaatgt tgtcgatgca gttatgttta
      721 gcgggttcgt ccgtacacat taaatttccc tgacagtcat aaagcagatt tttctcctgg
      781 ctttcaaaat aatatgcaaa tccgtcttga taagtgaatt tccacatctt atttacccgc
      841 tggttgttca tcagtcttcg cccccattca gttttaacag gatcaatcat atctatacat
      901 tgtagtttag agggttttgt tgtcttatct gcaatacaga tgctcaatat atctattatc
      961 atatcttcgg ccatgcaaat acctgctaca ttttgggctt ttttgtaagt aaatagtact
     1021 cgctgtccat ccatcaagct gaatgggatg acagtaaggt cattaggtag caattttttc
     1081 ccatcatctg taatcaggaa gtaggtacag ccatccaaat cccgcatatc tttcacagtt
     1141 gctcttgttt cttcctgaga cccggaattg gtgacaacag ttcgattgca agacaaaatg
     1201 agtaaaaatg tacagctcaa taggattttt tcaatcatac aaatactgca atatgctttt
     1261 tgtattaaat ttaaatatct ttgcggccta aaattaatta aaaaaacaat ttattttaaa
     1321 atttaaataa ttaaacgata tgagtggttc aaaaattacc attttcagag gaaaactcaa
     1381 agtgccggat aatccgatta tcccttttat tgaaggtgac ggtacagggc gcgacatctg
     1441 gagagcatct gtgagagtga tagatgctgc agtagaaaaa gcctataaag gcaagaaaaa
     1501 aatcgaatgg aaagaagtac ttgccggaga aaaagcattc aaacaaacag gcaactggct
     1561 cccggacgaa acacttgaca tcatccgcga gcatcttgta gccatcaaag gtccgcttac
     1621 tactccggta ggtggcggaa tcaccagcct caacgtagca ttgcgtcaga tacttgacct
     1681 gtatgtttgc ctcagaccgg tacagtattt tacaggcgtg ccttcgccgg tgaagcaccc
     1741 cgagaaagtg gatatggtta ttttcagaga gaatacagaa gacatctatg caggtatcga
     1801 attcacaggt ggttcggaag actctgataa aattctaaat tttataaaga aaaattaccc
     1861 taaatttttc tccaaaatac gattcggtac atccgcagcc gttaagagct ggaataaggc
     1921 aatcaaatcg aaagaagctg ctactgttga agtaggtatc ggcatcaaac ccgtgtctcg
     1981 agtaggtact gaaagattgg tgaaggctgc tattgactat gctattgcca acaatcgaaa
     2041 atcaataacc attgtacaca agggcaacat catgaaattc acggaaggag ggttccgtga
     2101 ctggggttac aaatatgctg agacacacta cagcaatcag acatatacct gggcacagtg
     2161 ggagcgcacc aagaaagaca aaggtgaaga agctgccaac aaagaacaag cagatgctct
     2221 ggcttctggc aagatcctta taaaagatgc cattgcagat atagctctac aacaggtact
     2281 tacacgtccg gatgactttg atgtgatagc aacgctaaac cttaacggag attatctatc
     2341 agatgctctt gcggctcaag tgggcggtat cggtattgca ccgggtgcca acatcaatta
     2401 cgttacgggt catgccattt tcgaggctac tcatggcact gctccaaaat atgctgacca
     2461 agacaaggtg aaccctggtt cggtgatact gagtgcagag atgatgctca gatatatggg
     2521 ctggactaaa gcagctgacc ttatattgaa ggggcttaac ggtgccatta gcagcaaaaa
     2581 cgtgacttac gatttccacc gtctgatgga aggcgccaaa cttgttaaat gttcggagtt
     2641 tggtgatgaa atcatcaaaa aaatgtaaac agaactgaat attactaaaa atggcatcag
     2701 atatatatcg ggtgccattt tttttaattg acctgaaaaa cctctatttt gacagtaatg
     2761 tttggcaagg cttcaatagc tgccaaatcg tactaacttt gtacaacatt catgaataac
     2821 aaatgaacag aatactgata atagacgacg aagaaaaact acgaactttg ctttcataaa
     2881 ttataacctt ggagggcttt gaagtattac aagcaggaga cattaaagcc gggctcaaaa
     2941 aattagaaac ggcattgatt gatgttgttt tgtgtgatgt aaaattacca gatggcagcg
     3001 gtattgatgc aaccaggatt ataaaggaaa aatatccgaa cattgaaatt atcttgctga
     3061 cagcctacgg caacataccg gacggagtac aagccatgaa aaacggagca tttgattata
     3121 ttacaaaagg cgatgacaac aataaaatta ttccattact atataaagca gtggagaaag
     3181 taacccttgc caaccgtgtc acattattag aaaaacaatt agagcataaa tattcgttcg
     3241 aaagtattgt aggaaagtca aaagcaatat tatcatcgat tgagtcggcc aggaaagtat
     3301 caacatctga ggcaactgtc ttattgaccg gcgaaaccgg tacaggaaaa gaagtttttg
     3361 ctcaggccat ccaccattca agcctgcgaa gcaaacacaa ctttatagca gtcaattgtt
     3421 cagccttcag caaggaattg ctcgaaaacg aattatttgg acataaagca ggtgctttta
     3481 ccggtgcgac aaaagacaca aaaggtattt ttgaagaggc caacaacgga acagtttttc
     3541 tggacgaact gggtgagatg cccctagatc tgcaggtaaa attattgaga gtgctggaga
     3601 caggcgaata tctgaaagtt ggcgacagca aaccaaccaa agtaaatgta c
//
LOCUS       DAOQQY010000280         3642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_12470_length_3642_cov_5.309730, whole genome shotgun sequence.
ACCESSION   DAOQQY010000280 DAOQQY010000000
VERSION     DAOQQY010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12470_length_3642_cov_5.309730"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLE23_11125"
     CDS             <1..842
                     /locus_tag="PLE23_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMX83859.1"
                     /translation="SLGLNILSYTEAILISIPLGFLVGLIPAVRGLFQKPVDSFRYVP
                     LTAVTGLFIVWFGLGTNMKVHFLAFGIIIYLLPVVVQRIDEVKEVFLLTVYTLGASKW
                     QTIKSVYIPSVISRLSDDIRVLTAISWTYIIIAESQGNEGGIGALIWRTGQRQGRIDK
                     LFALVIIIIIIGFLQDRLFTYLDKTFFPYKYEGVVKNSTNKEPSAFKAIMAYFFTALF
                     WVGMIVYGFLFVNEFVPLLSDVPVLTYLFGPTVWIMHFYFILVVIYQVYTRFISVKQT
                     VTT"
     gene            839..1519
                     /locus_tag="PLE23_11130"
     CDS             839..1519
                     /locus_tag="PLE23_11130"
                     /inference="COORDINATES: protein motif:HMM:NF015057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HMX83860.1"
                     /translation="MNFIKKLEKELKPEIDIILSHPFLKRIEKGDMSFEQLQYFAIEY
                     AEYCYHFPRFLAFTAGNIPDDLTRMPIIENLWEEHGSGKYSGSHRQLYNKFLEALDID
                     PRTLDKHPKLPDTITCVQTLLDMCKNDHFVKSLGALGPGTEFFTNDEYDLIFKGLDKS
                     PYLTQRELRFWKAHISLDVEHYSEMVEALYPYIHDENNCKLIKEGALKAIRMEQLFWD
                     GLERNIPV"
     gene            1532..2335
                     /locus_tag="PLE23_11135"
     CDS             1532..2335
                     /locus_tag="PLE23_11135"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX83861.1"
                     /translation="MSAETKTSLPAIIELKDVDQSYDDGKVQIIKGLNLTIEDKPAQG
                     QFTILLGMSGCGKSTLLRYIAGLQKPTSGTVLLHGKPVDADNRASMVFQEYSSLPWMT
                     VLDNVALPLEFKGIAKKQRYEQAMDMIEMVGLKGHESKYAQYPTLSGGQLQRVAIARS
                     LLANSDVLLMDEPFGALDINTRLQMQELLTDIWSKFHSTIIFVTHDISEAVFLGDEIV
                     IMKAPPSRIVQKIDVNLPLKRTREMKRDPKFLEIVHQIEDEMVSIANMK"
     gene            2357..3466
                     /locus_tag="PLE23_11140"
     CDS             2357..3466
                     /locus_tag="PLE23_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83862.1"
                     /translation="MKRFLFLFICSAFFFIPEQIHSQSSPYRLKVGSLNIPLKLVQPP
                     STYNGRKEVYLEDAVANIGGEIQLLNGDKPIYGKKLMIYFLPTKREQGTSLTSHTIIL
                     EKGAKIPDDVVAKFKEKVSIGDNFTIQTTGEGDDLNVNSASIYVKDPTQAYRPPVYPN
                     ANNDPDIFSWQMVGNLKKPLLKVDTAVADNKKIVKMYSDRSKYDLMPISGFQTKYRYV
                     KAGESFWPDNEISETHNFSTLKINPYYTYPEYAVPQEYAVSMRWGKINATPLSQNTTV
                     ADFLESCKNPIEISTPKDFIQLQQVEMIIVPDRGETVRYIVDNITNPKVQEALSKVND
                     RTTIYLQNAMVKNAKQELLYMPITFGFVISNTPKH"
BASE COUNT         1193 a          728 c          712 g         1009 t
ORIGIN      
        1 tttcacttgg cttgaatatc ctgagttata cagaagccat ccttatatct atacctttgg
       61 gttttttggt tggtctcatc ccggctgtgc gaggattgtt ccagaagccg gttgattcat
      121 tccgctatgt gccacttacc gccgttacag gtttatttat agtatggttt ggtcttggca
      181 ccaatatgaa ggttcacttc ctagcctttg gtatcatcat ttatctcttg cctgtggtag
      241 tacagcgtat agacgaagta aaagaggtat ttttactgac tgtatataca cttggcgcct
      301 caaagtggca aaccattaaa agtgtttata taccatcggt tatctccaga ctatcggatg
      361 atatacgcgt attgacagcc atcagctgga catacatcat cattgctgaa agccagggaa
      421 acgaaggcgg tataggtgct ctcatctggc gaacaggtca acgacaaggc cgtattgata
      481 aattgttcgc acttgttatc atcatcatta tcatcggatt tttacaggat aggttattca
      541 cgtatcttga caaaacattt ttcccataca aatacgaagg tgttgtaaaa aattcaacca
      601 acaaagaacc atcagctttt aaagcgatta tggcctattt tttcactgca ttattctggg
      661 taggtatgat agtttatggc tttctattcg ttaatgagtt tgtacctctg ttatccgatg
      721 tgcctgtgct tacgtatttg tttggcccaa cagtttggat catgcacttc tactttattc
      781 tggtggtaat ataccaggtt tatacaaggt ttatttcggt taagcaaaca gtgacaacat
      841 gaattttatt aaaaaattag aaaaggaatt aaagcccgag attgacatca ttctatcaca
      901 tccattcctg aaaagaatag agaaaggtga tatgagtttt gagcagttgc agtattttgc
      961 gattgaatat gccgaatatt gttatcattt cccaagattt ctggcattta cagccggtaa
     1021 cattcctgat gaccttacca gaatgcctat cattgaaaac ctatgggaag aacacggttc
     1081 aggtaaatat tcaggctcac acaggcagtt gtataataaa ttccttgaag ccctggatat
     1141 cgatccacgt acattggaca aacatccaaa actaccggat acaatcacct gcgtacagac
     1201 tctgttggat atgtgtaaaa atgaccattt cgttaaaagt cttggagcac tggggccggg
     1261 taccgaattt tttacaaatg atgaatatga tctaatattc aaaggcctcg ataagtcgcc
     1321 ctacctgaca caacgcgagc tacggttctg gaaggcacac atttcactcg atgtcgaaca
     1381 ttacagcgaa atggtagaag cactttaccc atatattcat gatgaaaata attgtaaact
     1441 gatcaaagaa ggcgccttaa aagccatccg gatggaacag ctgttttggg atgggctcga
     1501 gaggaatatt ccggtataaa taactgacaa gatgagtgca gaaaccaaga caagcctacc
     1561 ggcgataatt gaactaaaag acgttgacca gtcttatgat gacggaaaag tacagataat
     1621 aaaaggactt aacctgacga tagaagacaa acctgcacaa gggcaattca ccattttgct
     1681 tggtatgtcc ggttgtggga aatctacttt actacgttat atagccggac tgcaaaagcc
     1741 cacaagtggt acagtattat tgcatggcaa acctgtggat gctgacaatc gggctagtat
     1801 ggtttttcag gaatactctt ctcttccatg gatgaccgta cttgataatg tagcgcttcc
     1861 gctcgaattc aagggaatag caaaaaaaca acgctatgaa caagcaatgg atatgataga
     1921 aatggtaggt cttaaaggtc atgagtcgaa atatgcccaa tacccgactt tatccggtgg
     1981 ccaattacaa cgcgtggcca tagccagaag tcttctcgcc aactccgacg tattgctgat
     2041 ggacgaacca ttcggtgcat tggatatcaa tacccgcttg cagatgcagg aattattgac
     2101 agacatttgg tcaaaatttc attctaccat aatttttgtt acacacgaca tatcagaagc
     2161 tgtgtttttg ggagatgaaa tcgtcatcat gaaggcacca ccctctcgca tcgttcagaa
     2221 aattgatgtt aatttaccgt taaagaggac gcgcgaaatg aaaagagatc caaaattcct
     2281 cgaaattgtc caccaaatcg aagatgaaat ggtttcgatc gctaacatga aatgattttt
     2341 aattaaattt tttacaatga aaagattctt atttttattc atttgctctg catttttttt
     2401 tattcctgag caaatacatt cgcagtcaag cccttacaga ctaaaagtag gcagccttaa
     2461 tatccctcta aaactcgtac aaccaccaag cacctacaac ggccgaaaag aagtatatct
     2521 cgaggatgca gtagccaaca taggtggtga aatacaactg ctcaatggcg acaaaccaat
     2581 ctacggaaag aaactgatga tttatttcct gcctacaaaa cgtgaacaag gtacaagcct
     2641 cacatcacat actattatac tagaaaaagg agccaaaata ccggatgatg tagtggcaaa
     2701 attcaaggaa aaagtatcaa taggtgacaa ttttacaatt cagacaaccg gagaaggtga
     2761 cgacctgaat gtaaacagtg catccattta tgttaaagac ccaacccagg cttacagacc
     2821 accggtgtac ccaaatgcaa ataacgaccc ggatatattc tcatggcaaa tggttggcaa
     2881 cctgaaaaaa ccattgctaa aggtggatac agctgtagct gacaataaga aaatagtgaa
     2941 aatgtatagc gacagatcaa aatatgacct gatgcctatt agcggatttc agacaaaata
     3001 ccgctatgta aaagctggtg aaagtttctg gcctgacaac gaaatttcag aaacacacaa
     3061 cttttctacc ttgaagatta acccttacta cacttacccc gaatacgctg taccacagga
     3121 atatgccgta agtatgcgtt ggggaaaaat aaatgccacc cctctcagcc aaaatactac
     3181 ggtggcggat ttcctcgaaa gttgcaagaa ccctattgaa atatcaactc caaaagattt
     3241 tatacaactc caacaggtag agatgatcat tgtacctgac cgaggagaaa cggtgcgcta
     3301 catcgtggac aatatcacaa acccgaaagt acaggaagca ctttctaaag taaacgacag
     3361 gacaaccatc tatctccaga atgctatggt caaaaatgct aaacaagaat tgctttatat
     3421 gccaatcaca ttcggttttg tcatttccaa tacaccaaag cactaagcag taaaaagtaa
     3481 aaagttaaaa gcttctttta ctgaaaaacg ccacaagaga ttgtggcgtt ttttgtttgt
     3541 gaaaaaatgg aaagcggttg tttatggaaa attatttagc ctaaaattaa tttgattata
     3601 tactccaaaa aattttttac aaaaaaagca tacatgtata gt
//
LOCUS       DAOQQY010000281         3623 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_12564_length_3623_cov_7.498879, whole genome shotgun sequence.
ACCESSION   DAOQQY010000281 DAOQQY010000000
VERSION     DAOQQY010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12564_length_3623_cov_7.498879"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..1422
                     /gene="serC"
                     /locus_tag="PLE23_11145"
     CDS             352..1422
                     /gene="serC"
                     /locus_tag="PLE23_11145"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HMX83863.1"
                     /translation="MKKHNFSAGPGILPDEVIEKAADAVRDFNGSGLSILEISHRSPA
                     FEAVMDRSEALVRQLLGIGDDYGVIFLTGGASTQFFMVPMNLLHQDQKAVYADTGVWA
                     AKAIKEAKNFGDVEIIASSKPENYNHIPKKYSIPDDAAYFHITSNNTIYGTEYHSWPE
                     CEIPLVCDMSSDIFSRPVDGNRFGLIYAGAQKNMGPAGTTLVVVKKELLGKINRTLPS
                     MLDYRIHIENGSMYNTPPVYPIFVSMLTLEWILANGGTEQMAVRNKMKADLLYDEIDS
                     NPLFTGTAAIEDRSLMNVTFLLKDAALDKYFLEEVKKSGIEGIKGHRSVGGFRASIYN
                     AMPYESVRVLVDVMHDFAISKA"
     gene            1436..2191
                     /locus_tag="PLE23_11150"
     CDS             1436..2191
                     /locus_tag="PLE23_11150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HMX83864.1"
                     /translation="MGKILENVISYPDSVSVTLKVFKEPRMSWRISLGKNGAILRVPI
                     FMTKNELETKIEWSKDWIYEQLKSKPSLKSNFVHTRFRSGDTITITGKDYILEFVESE
                     KIKRLNARLKDNIIIISTPILSDKVFIDSVASLLSKVAAKISEPYIHRRVDELNDCYF
                     QKDICKITFKYNTSNWGSCSAKSNINLSTRLLRAPLAVIDYVIVHELCHLMHMNHSQE
                     YWALVQKIMPSYKIHEKWLRTNSHLCEFKPQSK"
     gene            complement(2275..2721)
                     /gene="rplI"
                     /locus_tag="PLE23_11155"
     CDS             complement(2275..2721)
                     /gene="rplI"
                     /locus_tag="PLE23_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMX83865.1"
                     /translation="MEIILLKDLDNVGEKHEIVKVKDGYGRNFLLPQGLAIVANSINK
                     KRLADFIRQDEAKESQRVNEYKEMVSKLKGVTLRIGAKAGKSGKIFGSVTNIQLANAI
                     KEQIGLEIERKKIHVPDDVKDLGTFTARIDLHKEVSCDVSFEVVGE"
     gene            complement(2750..3016)
                     /gene="rpsR"
                     /locus_tag="PLE23_11160"
     CDS             complement(2750..3016)
                     /gene="rpsR"
                     /locus_tag="PLE23_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMX83866.1"
                     /translation="MATRDDIKFLSNPNIGAQKKKYCRFKKFGIKYIDYKDADFLIQF
                     VNEQGKILPRRITGNSLKYQRKVATAVKRARHLAFIPYVADLLK"
     gene            complement(3042..3575)
                     /gene="rpsF"
                     /locus_tag="PLE23_11165"
     CDS             complement(3042..3575)
                     /gene="rpsF"
                     /locus_tag="PLE23_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMX83867.1"
                     /translation="MRNYEVTFIVDPVLSGDEIKSTAQTYLDMLAHEGCSIVHTDEMG
                     LRQLAYPINKRSSGVYFCIEFQSEQGNIIDKLELALRRDERIIRFLTVALDKFAVKYN
                     ADQREGKIGAAKKRIKPITTLEPIAPPAPVVQEAPAEKANVEANADQVVETVAAEPAQ
                     ENVEQTVTQESTESTEQ"
BASE COUNT         1142 a          702 c          661 g         1118 t
ORIGIN      
        1 tgcccctgtc gggtcgggct atgcgcctta cttcgtgccg gcttctatcc ctcacgcata
       61 tcactgcatt aggcgagaac taaggttaac acagatagca aattagaaat atacgaaaat
      121 gattattaca aataatccgt gaaaaagtat caaaagcaaa ttgaactatg ccttttgaaa
      181 tggatttgaa cgaattatta tttgcagcaa aaaacctaat gctgtatctg tgttaaacat
      241 tcatgcataa ttataggttc tatatatgac gtaataagca taccaaaata aataattttt
      301 cattattaca gaccaaatac tcatttttgc atactgaaaa attcagcctt tatgaaaaaa
      361 cataatttta gtgccggtcc cgggatattg cccgacgaag taattgaaaa agctgcagat
      421 gcggtccgtg attttaatgg ttcaggattg tcaatactcg aaataagcca ccgtagcccg
      481 gcctttgaag ctgtaatgga taggtctgag gctttggttc gtcagcttct tggtataggt
      541 gatgattatg gcgtcatttt ccttacaggg ggagccagca cacagttttt catggtgcca
      601 atgaatctgc tccaccagga tcaaaaggcc gtatatgctg ataccggtgt ttgggctgcc
      661 aaagctataa aagaagctaa aaattttggt gatgttgaaa tcattgcctc gtccaaacca
      721 gaaaattata atcatatacc caagaaatat tccattccag atgatgctgc ttattttcat
      781 atcacaagca acaacactat atatggtacc gaatatcact catggcctga atgcgagata
      841 ccacttgtat gcgacatgtc ttcagacata ttcagccgac cagtagatgg aaacagattt
      901 ggattaatct acgcaggtgc gcaaaaaaat atgggtcctg ccggcacaac acttgtagta
      961 gttaagaaag aattacttgg caagataaat cgtactctac catccatgct cgattaccgc
     1021 attcatatag aaaatggctc gatgtataat acacctcctg tttacccaat atttgtatct
     1081 atgttgacac ttgaatggat attggcaaat ggtggtacag aacaaatggc cgtcagaaat
     1141 aaaatgaagg cagatttgtt gtatgacgaa atagactcaa acccattatt caccggtact
     1201 gcagcaattg aagaccggtc gctgatgaat gttacgtttc tcttgaaaga tgctgcactc
     1261 gataaatatt ttctcgaaga ggttaaaaaa tcaggaatag aaggtattaa aggacacagg
     1321 tcggtaggag gattcagagc gtctatctat aatgcaatgc cttatgaaag tgtcagagta
     1381 cttgttgatg taatgcatga ttttgccatt tcaaaagcct aaacgaagta aaatcttggg
     1441 aaaaatactt gaaaacgtaa tttcataccc cgattctgtt tcagttacct taaaggtatt
     1501 caaagaacca aggatgtctt ggcgtatttc tttgggtaaa aatggagcaa tcttgagggt
     1561 cccgatattt atgaccaaaa atgaactcga gacaaaaata gaatggtcta aagattggat
     1621 ttacgaacag cttaagtcaa aaccttcatt gaagtccaat tttgtacata cacggttcag
     1681 gtcaggagat actattacca ttacaggaaa agactacatt ctcgaatttg tagaaagtga
     1741 aaaaattaaa cgcctaaatg ccaggttgaa ggacaatatc attattatca gtacacctat
     1801 attgtccgat aaagtcttca tcgattctgt tgcatctttg ctcagtaagg tagcggccaa
     1861 aatatctgag ccttacattc accgccgtgt ggatgaatta aatgattgct attttcaaaa
     1921 agatatttgt aaaatcacct ttaaatacaa cacaagcaac tggggaagct gttcagccaa
     1981 atcaaatata aatctatcta ccagactttt aagagctcct cttgcagtta tcgattatgt
     2041 gatagtacac gaactctgtc atctgatgca catgaatcat tctcaggaat attgggcact
     2101 tgttcagaaa attatgccat catataagat tcatgaaaaa tggctacgta ccaacagcca
     2161 tttgtgcgaa ttcaaaccac aatccaaatg aacggaatca aatcagaaag tctggatata
     2221 aaccaaaaaa cccggacgaa ccgggttttt tagtataaaa atgataataa ttacttactc
     2281 gcctacaact tcaaaactta catcacaaga tacttccttg tgcagatcaa ttctggcagt
     2341 aaatgttccg aggtctttta catcgtcagg tacgtgaatc ttcttgcgtt cgatttcaag
     2401 acctatctgt tctttaatgg catttgcaag ctggatattg gttacgctac cgaatatttt
     2461 accgctttta cctgccttag caccgattct caaagtaaca cctttaagtt tactgaccat
     2521 ctctttatat tcattaacac gttgagattc tttagcttca tcctgacgga taaaatcagc
     2581 aagtctcttt ttattgatag aattggcaac aatggctaat ccttgaggca atagaaaatt
     2641 tcttccataa ccatctttca ccttgacgat ttcatgtttt tcgccaacgt tatctaaatc
     2701 ttttaataat atgatttcca taaataattt cgtttccgga gttaaacaat tactttaaca
     2761 agtcagccac ataaggtata aaggcaagat gtcttgctct ttttacagca gtagctacct
     2821 ttctttggta tttcaatgaa ttacctgtga ttcttcttgg caaaatttta ccttgctcat
     2881 taacaaattg aataaggaaa tcagcatctt tataatcgat gtatttaatg ccaaattttt
     2941 taaatctaca gtattttttc ttctgagctc cgatattagg attgctcagg aatttgatat
     3001 catctctagt tgccattaca aatttgatta aagattaaca attattgttc tgtagattca
     3061 gtagattctt gagtcactgt ctgttctaca ttttcctgtg ccggctctgc agctacagtt
     3121 tctactacct gatctgcatt tgcttcaaca tttgcctttt cggcaggtgc ttcctgtacc
     3181 acaggtgcag gtggtgctat gggctcaagt gtagttattg gcttgattct cttctttgca
     3241 gcgccaattt ttccttcgcg ctgatcggca ttgtacttaa cagcaaactt gtcgagagca
     3301 acagttaaga aacggatgat tcgttcatct cttcttaatg caagttctag cttgtcaatg
     3361 atgttacctt gttctgactg gaactctatg caaaaataca caccagaaga acgtttgtta
     3421 atagggtagg ccaactgacg taggcccatc tcatcagtat gtacgatact gcacccttca
     3481 tgggcaagca tatcaagata cgtctgagct gtcgatttaa tttcatcgcc cgacagaact
     3541 ggatcaacga tgaaagttac ttcataatta cgcatgaaat agttttaaat tagttaataa
     3601 aaaattgcgg ctgcaaaggt aca
//
LOCUS       DAOQQY010000282         3599 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_12701_length_3599_cov_5.758183, whole genome shotgun sequence.
ACCESSION   DAOQQY010000282 DAOQQY010000000
VERSION     DAOQQY010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12701_length_3599_cov_5.758183"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="PLE23_11170"
     CDS             complement(<1..758)
                     /locus_tag="PLE23_11170"
                     /inference="COORDINATES: protein motif:HMM:NF017853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HMX83868.1"
                     /translation="MNLLDLLKDQIGSTVVNKAAEYLGESTSSTQSALGSIMPAVLGG
                     VINQASTTDGVKGLMGLFSKGSKHTDLLGNLGNMLGQTGGFDKILNMGGPIIKMLFGN
                     KTEGVFDLISNAAGVKKSSASGLMSLVAPVVMGLLSKKVSSEGLGISGLANFLMGQAG
                     SIKSALPAGFASQLGFSNLGDFLGDNIGGAASSGSTGGFGKFAPWLLGLLGLLLALYF
                     LKNCNKTEATDAANTAVENVTESATEVVDSGKTAV"
     gene            1006..2406
                     /locus_tag="PLE23_11175"
     CDS             1006..2406
                     /locus_tag="PLE23_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HMX83869.1"
                     /translation="MINRYTDLIHQTFDFPQESFEMVDGYLRFNDINLIELVRKYGTP
                     LKLTYVPKIGQQVEKARKFFKKAIKHSDYRGKYYYCYCTKSCHFSHVLDEVLAHGAHL
                     ETSSSFDIDLVWKLYSRGKIDEKTIIINNGFKTEEYTRKISDLINKGFKNIIPVLDNK
                     SEIFYYDQFVDTDEYNVGIRVATEEEPNFEFYTSRLGIRNADVLPFYRSVLSQNKRAR
                     LKMLHFFVDTGIKDSIYYWGELKKGITLYCELKKICPTLTALNIGGGMPIPNTLGFEY
                     DYQYMISEIVQLIKDSCKEYGIEEPDIYTEFGKYTVGESGAVILSVVNAKKQNDSEIW
                     YMVDNSLMNTIPDSWGIGERFILLPINKWNNGFRRVNIGGLSCDNSDYYNAEVHENQV
                     YLPVNEDDSDEPLYIGFFHTGAYQDALSGYGGIKHCLIPSPKHIIVDVDDNGKLTDRV
                     LFPEQTAESMLSILGY"
     gene            2411..3403
                     /locus_tag="PLE23_11180"
     CDS             2411..3403
                     /locus_tag="PLE23_11180"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF005486.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HMX83870.1"
                     /translation="MIGRADFLTEVQARQRMNELSSQAVPYIFIIDFLKENSLVIPLA
                     ALHSDSIQYDFSGFTNAVQSKIENREVEFKKFPQSFEDFEKSFKLVMEEILYGNSFLL
                     NLTCQTPIEINFSLGEIFQKSKARYKLLLQGSYVCFSPETFVRIKDKHIYSYPMKGTI
                     DASIEDAENIILNDPKEKAEHYTIVDLIRNDLSMVSDKVRVTKFRYIDRLKTNFGEIL
                     QVSSEVEGTLRDGYTAHLGDIIFTLLPAGSISGAPKERTVQIIRSAETYDRGFYTGIA
                     GIFDGENLDSTVLIRFIEEQDGRKYFKSGGGITFMSDARAEYEEMISKVYVPFS"
     gene            3387..>3599
                     /locus_tag="PLE23_11185"
     CDS             3387..>3599
                     /locus_tag="PLE23_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83871.1"
                     /translation="MFRFLETIRIKDGTKQLDGYHLKRMKRTLGTVPLPFSVIEFSKI
                     LQVPKNLQSVTVKARLLYDANGYDIGF"
BASE COUNT         1163 a          689 c          763 g          984 t
ORIGIN      
        1 actgctgttt tgcctgaatc aaccacttca gtagcagact ctgtaacatt ttcaactgct
       61 gtgttagctg catcagttgc ttcagttttg ttacagttct taagaaagta aagtgcgagc
      121 aacagaccaa gcaagccaag gagccatgga gcaaatttgc caaagccacc tgtagaaccg
      181 gaagaagctg caccgcctat gttgtcacct aagaagtcac ccaggttaga gaagcctaac
      241 tggctggcaa aacctgcagg caatgcactc ttaatagagc ctgcttgacc catcaagaaa
      301 ttagcaagac ctgagatgcc aagaccttca gacgatactt tctttgacaa caagcccatt
      361 actacaggtg ctacaaggct cataaggcct gatgcagaac ttttctttac acctgctgcg
      421 tttgaaatca aatcaaatac accttcagtt ttgttgccga acaacatttt gatgatcggg
      481 ccacccatat tgaggatttt gtcaaagcct cctgtctgcc ctagcatatt gccaagattg
      541 cctaataaat ctgtgtgttt gctgcctttt gagaataggc ccatcagacc ttttacgcca
      601 tcagttgtag atgcctggtt tattacgcca ccaagtactg caggcatgat agagccaagt
      661 gcagactgag tgctggatgt tgattcgcca aggtattctg cagctttgtt aacaactgta
      721 gagccaatct gatcttttaa caagtctagt aaattcatag tcaaatgttt taaaaaaaat
      781 gattaaaaaa tggtgttaat tatgggggaa aataatttgc agaaaaataa tcttttgagc
      841 gttaaatata tcaatataca ttattgagtg cgaaggtatt ttattattaa tgttgggtac
      901 acactataga cgcagattaa caaaaaagta acaaaatatt aaaacaatcc tgcaagtaag
      961 caaagtatcc cttatttttg tttttcctta aaaaaatgca acataatgat taacagatat
     1021 accgacctta ttcatcagac ttttgatttt ccacaagaga gctttgaaat ggtggatggt
     1081 taccttcggt tcaatgatat aaacctgata gaactggtgc ggaaatatgg tacaccgctt
     1141 aaactaacct acgtgcccaa aatcggacaa caggtagaaa aggcgaggaa atttttcaag
     1201 aaagccatta agcattctga ctatcgtggc aaatattact attgctactg cacaaaaagc
     1261 tgccatttca gtcatgtact cgatgaggtt cttgcccatg gtgctcatct agagacatcg
     1321 tcttcgtttg atattgatct ggtatggaag ctatatagcc gaggcaaaat cgacgaaaag
     1381 accatcatca ttaacaatgg attcaaaacc gaggaatata cccgcaagat tagtgaccta
     1441 atcaacaaag gatttaagaa catcataccg gtgctcgaca ataaaagtga aatattttac
     1501 tacgatcagt ttgtagatac ggatgagtat aatgtaggta tcagggtagc aaccgaagaa
     1561 gagccgaact tcgagtttta taccagccga ttgggtattc gcaatgctga tgttctgccg
     1621 ttttatagaa gtgtactttc tcaaaacaaa agagcaaggt taaaaatgct acatttcttt
     1681 gtagatacag gtatcaaaga ctctatctac tactggggcg aacttaagaa aggcataacg
     1741 ctttattgtg aacttaaaaa aatatgccct acactgacag ccctaaacat cggcggtggc
     1801 atgccaatac ctaacacact cggatttgaa tacgactacc agtatatgat ttcggagatt
     1861 gttcaattaa taaaagactc gtgcaaagaa tatggtatcg aagagccgga catctatacc
     1921 gaattcggga aatatacagt tggcgaaagt ggagccgtaa tcctctctgt ggtaaatgca
     1981 aagaagcaaa atgattcgga aatctggtac atggtcgata attcgcttat gaacaccatt
     2041 cctgatagtt ggggtatcgg tgagcggttc atcttgttgc ctattaacaa atggaacaat
     2101 ggatttcgca gggtaaatat cggtgggctt agctgcgata actcagatta ttacaacgct
     2161 gaagttcatg aaaaccaggt gtacctgcct gtgaacgaag acgatagtga cgagccactc
     2221 tacatcggtt tttttcacac aggtgcttac caagatgcat taagtggcta cgggggcatc
     2281 aagcattgcc tcatcccgtc accaaagcat attatcgtag atgtggacga taatggtaag
     2341 ctgaccgacc gggttttgtt tccggagcaa acggccgaat ccatgttgtc gatactcgga
     2401 tactgatctg atgataggca gggcagattt ccttacagag gtacaggcga gacagcgtat
     2461 gaatgaattg tcgtcgcagg cagtaccata tatatttatt attgatttcc tgaaggaaaa
     2521 ttcactcgtc atcccacttg cagcgcttca ttcggacagc atccagtatg atttcagtgg
     2581 gtttaccaat gcagtacagt caaagataga gaaccgggaa gtagaattta agaaatttcc
     2641 acagagcttt gaagactttg aaaaatcttt caagcttgtg atggaagaaa tattgtacgg
     2701 aaacagtttt ttactcaatc ttacctgcca aacaccaata gaaatcaatt ttagtctggg
     2761 agaaatattt caaaagtcaa aagcaaggta caaactacta ttgcaaggca gttatgtttg
     2821 cttttcgccg gaaacattcg ttcgaatcaa ggataagcac atttattctt acccaatgaa
     2881 aggaacaata gatgcaagta tcgaggatgc ggaaaatatc atcttgaacg atcctaaaga
     2941 aaaagccgaa cattatacca tcgttgacct gatacgcaat gacctgagta tggtgtcaga
     3001 taaggtaagg gtaacaaaat ttaggtatat tgacaggctg aaaaccaatt ttggtgaaat
     3061 tcttcaggtg agttccgaag tagaaggcac actcagagat ggttatacag cacaccttgg
     3121 agatattatt tttacactgc tgcctgccgg ttccatctcc ggtgctccca aagaacgaac
     3181 agtgcagatt atccgatcgg ctgagactta tgacagaggc ttctatacag gtattgctgg
     3241 aatattcgat ggagaaaatc tggatagcac agtactaata aggttcatag aagagcagga
     3301 tggaaggaaa tattttaaaa gcggtggtgg tattactttt atgagtgatg caagggcaga
     3361 atatgaagaa atgataagta aagtatatgt tccgttttct tgaaaccata agaataaaag
     3421 acggtaccaa acagctggac gggtaccatc tcaaacggat gaaacgcacc ttgggtactg
     3481 tgccgcttcc tttttcagtg attgaattta gtaaaatact acaagtgccg aaaaacctgc
     3541 aatccgttac cgtaaaggca cgattattat atgatgcaaa tggttatgat attgggttc
//
LOCUS       DAOQQY010000283         3597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_12709_length_3597_cov_5.420384, whole genome shotgun sequence.
ACCESSION   DAOQQY010000283 DAOQQY010000000
VERSION     DAOQQY010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12709_length_3597_cov_5.420384"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="PLE23_11190"
     CDS             <1..672
                     /locus_tag="PLE23_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX83872.1"
                     /translation="TLILAFASLLFALLIGIPLGIICALNRDKMADRAIIAFSTLGVS
                     LPSYIPAIMFAVFFGYVWSSLTGLDVQGSIIELNDLGNEEIRIRNLILPVLALGIRPV
                     AMITQLSRSAFLDVMNQEYIKTAKAKGVRLVSLLYRHALPNASITIISASSGWLATLI
                     AGSYFVESIFNFKGIGDLTISSLTSFDIPVILGTLITVCIFFIIINLVVDMLYTKVDP
                     RIKVN"
     gene            691..1317
                     /gene="yihA"
                     /locus_tag="PLE23_11195"
     CDS             691..1317
                     /gene="yihA"
                     /locus_tag="PLE23_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMX83873.1"
                     /translation="MIIKEIEFIGSFPKADKMPSDLIPEFAFIGRSNVGKSSLINMLC
                     NHDKLAKVSSTPGKTQLINVFDINKRLKIIDLPGFGYAKVSQKLRESWSKMTEYYLLN
                     RENLTCIFILIDCRLEPQKIDVEFIRWCAENGLPFALVYTKADKIKMNQIMKNIQEMK
                     GILQQDFQNMPDIIITSAENKRGKEEVWNYIENVLIAFEKHIHENKIN"
     gene            1298..2998
                     /locus_tag="PLE23_11200"
     CDS             1298..2998
                     /locus_tag="PLE23_11200"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMX83874.1"
                     /translation="MKTKSINPLKSKEHLGLEKWPIAIQDKRRGELVEEIIEETLSKY
                     QAMDNESLESVINKSIYLERQRLKAEPWKVDPPNEHVFWNKSKRKLEQALRIEDKQEQ
                     KQKLLENIEKLIRRYTEEIVGNFNIETFFFARKFLTVFFKGLFDHFSFLDFFAQRRAR
                     IRCLRHFHVKGDISKIKQLFKHGTVVILPTHSSNLDSILIGYMIDQVTKLPAFSYGAG
                     LNLYNNSLAAFYMNRLGAYRVDRRKKNPIYLDTLKNMASISVEDGTNSIFFPGGTRSR
                     DGIVETRLKLGLLGSVIDAQRSSLEKGHNNKIFIVPLVVSYETVLEAGMLIEQHLKRS
                     GREQYTSKIKRNCDFFKILKFARNLIFRKNDVFFSFGEPIDVIGNVVDHNGGSMDKYG
                     NIIQVKSYFELNGKVNKDEQRESEYTKLLGKKIGEMFLRENILLPTNFLCYLVVNLLK
                     ERHDTSEIFDILRMPVKGWWVDRALLYENAQLLLQIFRQKEKEGIFHLSEHFKMTADK
                     IIEHALRFGGNYHVIKPMKEEQRNNKLIINDLPVVIFYANRLDGYELEGQIKWLKEVS
                     "
     gene            3025..3558
                     /locus_tag="PLE23_11205"
     CDS             3025..3558
                     /locus_tag="PLE23_11205"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMX83875.1"
                     /translation="MVRIVFDLEATCWLEQNKNFRQEIIEIGAYKLDEYGEVIDKFQQ
                     FVRPQVHPYLSSYCTQLTSISQKNVDRAKYFPVVQDLFLAWIDQDEYKLISWGRSDIR
                     LLKDDCELYKIDTGWLDGKHIDLKDEYKRLKQLKKRIGFQRALELEGMNFVGTQHRAI
                     YDAANLVQLYTKYIDVW"
BASE COUNT         1220 a          600 c          708 g         1069 t
ORIGIN      
        1 acgctgattc ttgcgtttgc ttccttatta tttgcattac tgatcgggat tccacttggc
       61 attatttgtg cattaaaccg agacaaaatg gccgacaggg caattattgc tttttcaacc
      121 cttggtgtga gcctaccaag ttatattccg gccataatgt tcgcagtatt tttcggatac
      181 gtttggtcta gtcttacagg tttggatgta caaggcagta taatagaatt gaatgatttg
      241 gggaacgaag agattcgtat tcgcaacctc attttaccag tattggcact tggcatcaga
      301 ccggtagcca tgattactca actatcccgc agtgcatttc tcgatgtcat gaaccaggaa
      361 tatatcaaaa ctgcaaaggc aaaaggtgtt cggttagtaa gtctattgta cagacatgca
      421 ttgcccaatg caagtattac cattatttct gcttcatcag gttggttggc gaccctaatt
      481 gccggctcat attttgtcga atcaatattc aattttaaag gtatcggtga tttgaccatt
      541 tcttcattga caagttttga cattcctgtc attttgggta cacttattac agtatgtata
      601 tttttcatta ttattaacct tgtggttgac atgttatata ccaaagttga ccctagaata
      661 aaagtgaatt aatattaaat ctacatggcc atgattatta aagaaataga atttattggg
      721 tcttttccaa aagcggataa aatgccttcc gatttgattc cggagtttgc gtttataggc
      781 aggtctaatg tcggtaaatc gtctctgata aacatgttat gcaatcatga caagctagcc
      841 aaggtatcat caacacccgg caaaactcag ctcatcaatg tatttgacat aaacaaacgc
      901 ttaaagatta ttgatttgcc tggcttcgga tatgccaaag tctctcaaaa actgcgtgag
      961 tcatggagca aaatgaccga atattattta ttgaacagag aaaatctgac ttgcattttt
     1021 atcttgattg attgcagact tgagccacaa aagattgatg ttgaatttat tagatggtgt
     1081 gcagaaaatg gtctcccatt cgcattggta tatacaaaag ccgataagat taaaatgaac
     1141 caaattatga agaatattca agaaatgaaa ggtattttgc aacaggattt tcagaacatg
     1201 cctgatataa tcattacgtc agcagagaat aaacgaggaa aagaagaagt atggaactat
     1261 atagaaaatg ttttgattgc ttttgaaaaa cacattcatg aaaacaaaat caattaatcc
     1321 gctcaaatca aaggaacacc tgggtcttga aaaatggccc atagccatcc aggataagag
     1381 aaggggagag ctggtagaag aaattattga ggaaacactt agcaaatatc aagcgatgga
     1441 caacgaatcg ctcgaatctg ttatcaataa atctatttat ctcgaaagac aaagattaaa
     1501 agccgaaccc tggaaagtcg atccacccaa tgaacatgtt ttctggaaca aatcgaaaag
     1561 aaaattggaa caggcacttc gcattgaaga taaacaagag caaaagcaaa aattattaga
     1621 gaatatagaa aaactcataa gaagatatac tgaagaaatc gttgggaact ttaatatcga
     1681 aacttttttc tttgccagaa aatttttaac agtatttttt aaaggtcttt ttgatcattt
     1741 tagttttctt gattttttcg cccaaagaag agcacgtatc agatgcctca gacattttca
     1801 tgtaaaaggt gatatctcaa aaataaaaca actgttcaaa cacggtacgg ttgtcattct
     1861 gcctacacat tcgtctaatc tcgattcgat actcattggg tatatgattg accaagtcac
     1921 caaactacct gcattttcgt atggtgccgg acttaattta tacaacaata gcttggcagc
     1981 tttttatatg aatcgacttg gcgcataccg tgtggacaga aggaaaaaga atccgattta
     2041 tcttgacaca cttaaaaaca tggcaagtat ctcggtagaa gatggtacca attctatttt
     2101 cttccctggt ggcacacggt cacgtgatgg aatagtagag acccgactta aactaggctt
     2161 gcttggtagc gtcatcgatg ctcagcgttc ttcacttgaa aaagggcaca ataacaaaat
     2221 atttatcgta ccgctggtag tttcatacga gactgttcta gaagccggaa tgttaataga
     2281 acaacacctt aaacgttctg gtcgcgaaca atatacttct aagattaaaa gaaattgtga
     2341 tttctttaag atcttgaagt ttgccagaaa tctgattttt agaaaaaatg atgttttctt
     2401 ttcatttgga gaacctattg atgtgattgg aaatgtggtt gaccacaatg gcggcagcat
     2461 ggacaaatac ggaaatatca ttcaggtcaa atcatatttt gaactaaacg gaaaggtcaa
     2521 taaagacgaa caacgcgagt cggaatacac aaaacttttg ggtaagaaaa taggagagat
     2581 gtttctaagg gaaaatattc ttttgcctac aaattttcta tgctatttag ttgtcaatct
     2641 tctaaaggaa aggcatgata caagtgaaat attcgacatt cttcgtatgc cggtaaaagg
     2701 atggtgggtg gatagagcct tgttgtatga aaatgctcaa cttttattgc aaatatttag
     2761 acaaaaggaa aaggaaggca tttttcatct ttcagaacat tttaaaatga ctgcagataa
     2821 aataattgaa catgcattac gcttcggagg taattatcat gtcattaaac caatgaagga
     2881 agaacaacgc aataataaat taattatcaa tgatttacct gttgttattt tttatgccaa
     2941 cagattagat ggttacgagt tggaaggtca gataaaatgg ttgaaagaag ttagctaaac
     3001 gaaattgttt gaaaaggagt ttaaatggta agaattgtat ttgatctgga agctacttgc
     3061 tggctggaac aaaacaaaaa tttcaggcag gaaatcattg aaataggtgc ttacaagctg
     3121 gatgaatacg gcgaagtaat tgacaaattt caacaatttg tcaggcctca ggtgcacccg
     3181 tatctatcat cgtattgtac acaacttacc agtattagcc aaaaaaatgt ggacagagca
     3241 aaatattttc ctgttgtgca ggatttattt cttgcctgga tagatcaaga tgaatataaa
     3301 ctcatcagtt ggggccgttc ggacatacgc ttactaaagg atgactgcga attgtataaa
     3361 attgacacgg gctggcttga cggaaaacat attgatctaa aagatgaata caaacgattg
     3421 aaacaattaa agaagcggat tggatttcag agagcgctcg agctggaagg aatgaatttc
     3481 gttggcacac aacaccgagc catttacgat gctgcgaact tagtccaact ttacacaaaa
     3541 tacattgacg tctggtagaa aagtgataag ccgaattttc tatttaacat aatataa
//
LOCUS       DAOQQY010000284         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13144_length_3521_cov_5.255049, whole genome shotgun sequence.
ACCESSION   DAOQQY010000284 DAOQQY010000000
VERSION     DAOQQY010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13144_length_3521_cov_5.255049"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /gene="ribB"
                     /locus_tag="PLE23_11210"
     CDS             complement(<1..1104)
                     /gene="ribB"
                     /locus_tag="PLE23_11210"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HMX83876.1"
                     /translation="MSGNQLNDIKLNTPREAIEDIKQGKVIIVVDDEDRENEGDFICA
                     AEKITPEIINFMATHGRGLICTPIDEHRATELNLNMMVSNNTAAHETAFTVSVDLLGH
                     GCTTGISAYDRATGIKAFTNPDIKAGDFARPGHIFPLIAKTGGVLRRTGHTEAAVDLA
                     KLAGFYPAGVLVEILNGDGSMARLPELLKIAKKFDLKIISIKDLVAYRMIHERIIKKE
                     LVSKLSSEYGEMELHLYHDLTTDDTHYAIKYGDWKDDDDVPVRVQSVRSFAELLKFLL
                     HPTSSDVHKAFNIIVERHKGLFLMMKQQDHFKNPQQEMEYLGNNFQTGRPKRDAALGQ
                     RDFGIGAQIIRDLGISKIELITHNMQKRIGLEGY"
     gene            complement(1076..2266)
                     /locus_tag="PLE23_11215"
     CDS             complement(1076..2266)
                     /locus_tag="PLE23_11215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX83877.1"
                     /translation="MKLLLLVILIISFTLILHTYLLYPLICFVWSKIKSIPNNIHSDI
                     NALPFVSFIMPLHNEEKVLRQKIESLINLNYPTHKIKWYFGSDASTDQTNAILSTWED
                     RQHVFYKNTRTGKPGMVNFLVEKAIANYAAGAEHILLFTDASVMLDSDCLINLVKHFK
                     NPDMGLVDANMINKGVNKNNISAAESRYVSLEVKLKHWESKINRKMIGPFGGCFALRS
                     DFFSPIPNNFLVDDFYLCMEVFRKGGHAINALDAISYESVSEEIQEEFRRKVRIGAGN
                     LQNMVYFHSFLSKPFLPISFFIYSHKVIRWFVPVLAILVFLTLTGLSMIEPNPYFLIL
                     LVLIFATAGIPLVEFLLNKLKIVVLPVKNLNYFIWMNLALLKGIINYYKGIKTNVWQP
                     TKRY"
     gene            complement(2280..3197)
                     /locus_tag="PLE23_11220"
     CDS             complement(2280..3197)
                     /locus_tag="PLE23_11220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMX83878.1"
                     /translation="MLIQILQKNEFDIIQLETLYLAPYIPAIRKHSKAQISMRAHNVE
                     FEIWQRITEHTTNPLKKWYLNHLTSKLKKYEIAQLVNYDILVPITDRDAKIFASLGFK
                     GKMQVVPIGIDKNDYVANNSSFDRELSLSFIGSLDWLPNIEGLNFFLHEIWPVLHKKF
                     PKLTFHVAGRNTPESLKSKNLPGVVFEGEVADAVEFLNKHSVMLVPILSGSGMRAKIL
                     EGMALGKVVITTTIGLEGNEARHKDHLLVADNTDDFVDCIEYCYKQKDNLAKIGQNAQ
                     SFIFEQHHNLELAKRLMNRYKSPSLIAVS"
BASE COUNT         1057 a          678 c          655 g         1131 t
ORIGIN      
        1 ataaccttcc aggccaatac gtttttgcat attatgcgtg atgagctcga tctttgaaat
       61 gcccaggtct ctaataattt gtgcaccaat tccaaaatcg cgctgaccca gagcagcatc
      121 tcttttgggc cttccagttt gaaaattatt tccaaggtat tccatttcct gttgagggtt
      181 cttgaaatga tcctgttgct tcatcatcaa aaacaaacct ttgtgccttt ctactatgat
      241 gttaaaagct ttgtgtacat cacttgaagt aggatgtagt aaaaatttca gtaattctgc
      301 gaatgaccgg acactttgga ctcttacagg tacgtcatca tcatctttcc agtctccgta
      361 ttttatagca tagtgtgtat catctgtggt tagatcgtga tacagatgta gttccatttc
      421 gccatactca ctggagagtt tagatacgag ttcttttttt atgatccgct catgaatcat
      481 cctgtaagct acaagatctt ttattgaaat aattttaagg tcaaatttct ttgctatctt
      541 caatagctct ggaagtcggg ccatgctacc gtcgccgttg agtatttcga ccaagacacc
      601 ggcagggtaa aaaccggcaa gcttggcaag gtcaacagcg gcctcggtat gtcctgttcg
      661 cctcaatacg ccaccagtct tggcaatcaa cggaaaaata tgtccggggc gggcaaaatc
      721 tcctgcttta atatcagggt tggtaaatgc tttgatgccc gttgctctgt cgtaggcaga
      781 aatgccggtg gtacatccgt gacccagcaa atcaaccgac acagtaaatg ccgtttcgtg
      841 ggcggccgta ttattactta ccatcatatt gagattaagc tctgtagccc tgtgttcatc
      901 tatcggggta catattaacc ctcgcccatg agtagccata aaattaataa tctcaggtgt
      961 tatcttttct gcagcacaaa taaaatcacc ttcattttca cggtcttcat cgtccaccac
     1021 gatgattact tttccttgct ttatatcttc aatagcttcc cgaggagtat tgagtttaat
     1081 atcgtttagt tggttgccag acattggttt ttattccttt gtaatagtta atgattcctt
     1141 tcagaagtgc aagattcatc cagatgaaat aattcagatt tttgaccggc aaaactacta
     1201 tttttaactt atttaataga aattcgacca gaggaattcc tgcagtagca aaaattaaaa
     1261 ctaataaaat caaaaaatat gggtttggtt caatcatact caatccagta agagtaagaa
     1321 atactaaaat ggccaaaaca ggtacaaacc acctgatcac tttgtgtgaa tagataaaaa
     1381 agctaattgg taaaaatggt ttggagagaa aactatggaa gtaaaccata ttttgcaagt
     1441 tgccggcacc tatacgtact tttcttctga attcttcttg tatttcttca gatacagatt
     1501 cataactgat tgcatcaagc gcatttatag catggccacc ttttctgaaa acttccatac
     1561 acaagtaaaa atcatctacc aggaaattat tcggtatggg tgaaaagaaa tcactacgca
     1621 acgcaaaaca acctccgaat ggacctatca tttttcgatt gattttgctt tcccaatgct
     1681 tcaattttac ctcaagtgaa acatatctgc tttctgctgc agaaatgtta tttttgttta
     1741 cacctttatt aatcatgtta gcatctacaa gccccatatc ggggttttta aaatgcttta
     1801 caagattaat caggcaatcg ctgtctagca tcacacttgc atctgtaaac aataatatgt
     1861 gctctgcacc ggctgcataa ttggcaatag ctttttcaac aagaaaattg accattccgg
     1921 gttttcctgt tcttgtattt ttgtaaaaaa catgctgacg gtcttcccat gtactaagta
     1981 ttgcattcgt ctggtctgta gaagcatccg atccgaaata ccactttatt ttgtgagtgg
     2041 gatagttcag atttatgagt gactcaatct tttgtcttag cactttttct tcattgtgta
     2101 atggcatgat aaatgacacg aaaggcaatg cgtttatatc agaatgaatg ttatttggta
     2161 ttgactttat tttgctccat acaaaacaaa tcaaaggata caaaagataa gtatgcaaga
     2221 taagagtaaa cgaaataata agtataacca gtaataaaag tttcatctat acatatacat
     2281 catgatactg caataaggct tgggctttta tatctgttca tcagccgttt tgcgagttcg
     2341 aggttatggt gttgttcaaa aataaatgac tgggcattct ggcctatttt tgcaagatta
     2401 tctttttgct tataacaata ttcaatacag tcaacaaaat catctgtatt atctgcaacc
     2461 agtagatgat ctttatgtct cgcctcattg ccttcaagtc ctatggttgt agtgatgact
     2521 actttgccaa gcgccatacc ttccagtatt tttgctctca taccgctgcc cgacaatatt
     2581 ggtacaagca ttacagaatg tttattcaaa aattcgactg catcagcaac ttcaccttca
     2641 aatactacac ctggcagatt ttttgatttg agtgattcgg gtgtattcct gcctgcaacg
     2701 tgaaatgtta gtttggggaa tttcttgtgc agtaccggcc atatttcatg taggaaaaaa
     2761 ttgagacctt cgatatttgg aagccagtcg agtgaaccta tgaaagataa actcaattcc
     2821 ctgtcaaacg aactgttgtt ggctacataa tcgtttttat ctattccgat cgggactacc
     2881 tgcattttgc ctttaaaacc taatgaagcg aatatcttag catctctgtc agtaatgggg
     2941 acgagtatgt catagttaac aagttgggct atttcatatt tctttaattt cgaagtaagg
     3001 tgattcaggt accatttttt gagtggatta gtggtgtgtt cggttattcg ttgccatatt
     3061 tcaaactcca cattgtgggc acgcatactt atttgagcct tagaatgttt ccttatggca
     3121 ggaatgtaag gtgccagata tagtgtctca agctgtatga tgtcaaattc gtttttctga
     3181 agtatttgta ttaacaattt ctcaaaactc cttgaaatat agcgagatat atgaaatgat
     3241 tctgatgaaa aaagattgag aaatgcaaaa caggaaatta cggaggtaaa aactttagac
     3301 tactacgagc acatagtaaa cggggagata cgacaaaccc agaaagaact gatcgaatat
     3361 gtgaataagc aatacgggct caacttaacg ccctgaatga tgtatgtgtc cataacgatt
     3421 ttaattgcca taaatcgaat atataatttt aagtgaatac gaagatgggt ggcaacaaga
     3481 aactggaccc ctacatgaaa gacgccaaga aaatgaggaa g
//
LOCUS       DAOQQY010000285         3492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13332_length_3492_cov_5.993599, whole genome shotgun sequence.
ACCESSION   DAOQQY010000285 DAOQQY010000000
VERSION     DAOQQY010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13332_length_3492_cov_5.993599"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="PLE23_11225"
     CDS             complement(<1..1164)
                     /locus_tag="PLE23_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83879.1"
                     /translation="MKLICISIILCGFNQQSFAQKYTFQKIYKRDFPTQTLNCTALKD
                     GGYAMIGIADTTGGSFVRLTKLKCDGEVEWVREFGTSSTANNVSPRVIDTRENDIVVV
                     SNTGSFNNYAMIVIRIGYDGFVKWKKRMVTVGETRGQALVETQDGGFAVAGMTKSYGT
                     DVLNNWSDVYLVKFDATGEVLWSKTYGNNKNIDEAYGIAEDAYGNLLTTGRYIVDGTF
                     YSFLLKTNAEGVAKFLKAYGAANQSTTGYDVLVTKDQKNYVITGGTTINKSSFQDYHD
                     VFVIKTDTAGNPVFSKIYYTISGTDGSDSGTSVVEESTGDFAIGVATFSFSNHTVGFV
                     PNKNAVFRISPVGNLNKAVLYNQGGSHYTKLQPAYDGGYILSAFTNLNISQGLF"
     gene            1277..2569
                     /locus_tag="PLE23_11230"
     CDS             1277..2569
                     /locus_tag="PLE23_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMX83880.1"
                     /translation="MVNRRKFFQIAGSTALAMSPVTRMLCKTDERAVRQNFNLPDYYP
                     IEESIEDEDFWYQVRMAYTVFPNLINLNNGGVSPSPKIVQEAVERYNKLTNEAPSYYM
                     WRILDQGREPLREKLALLAGCDTDEIAINRNSSEALETIIFGLRLKQGDEVVLTRQDY
                     PNMINAWKQREKRDGIVLKWIDLPLPMENDDQIVQKYSEQFSTKTKIVHITHIINWTG
                     QIIPARKIADIAHQKGIDVLVDAAHSFVHIGYKIPDLDCDYFGTSLHKWLCAPIGSGM
                     LYVKKEKISQLYPLFAAGDPESADIRKFENLGTRSFPIEQGISEAIDFQNMLGMERKE
                     KRLRYLKNYWTTKVQDLPGVKLNTSLKDNYSCAIANFSIEGKKPEEIENTLFSKYKIH
                     TVAINWENIHGVRVTPNVYTLTSDLDKLVKGIREIIKS"
     gene            complement(2579..>3492)
                     /locus_tag="PLE23_11235"
     CDS             complement(2579..>3492)
                     /locus_tag="PLE23_11235"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMX83881.1"
                     /translation="ISTQAGESVQKLLSDAAGELKINLTKDKMYFFKTMSDNFKSESK
                     LVNTSAKSEEKIKMIVTLEPTEAYFKQLKNEANKNYRSKGLYAVQVGVVKNSTPIDYA
                     KYNKIVKGAIAYTTEDDGQKSKLRIGNFATKADAQTAIRLLTQNDVKGCFIVEDKEYV
                     SVKKAEEPVAAKPEENKPAKVKPSEPKVVTKVPETKAVDVKPAQAEAKPIAVKPATVV
                     KEEPKPEDNFRVRIAAVRDPKFSDDPKLAALGIVLTEPAGEWTRVYLGYYKDLTGAKA
                     SLENAMKAGFKDAYVCTKKGNTWAKVK"
BASE COUNT         1011 a          761 c          687 g         1033 t
ORIGIN      
        1 aaagagtccc tgactgatat tgagatttgt aaaagcagac aagatatagc caccgtcata
       61 agccggctgc agtttggtgt aatgcgaacc tccctgattg tacaaaacgg ccttatttag
      121 attgcccacc gggcttatcc tgaacactgc attcttgttt ggcacaaaac cgacagtatg
      181 gtttgaaaac gaaaatgtag caacaccaat tgcaaaatcg cctgttgatt cctccaccac
      241 agaagtgccg ctatccgatc catccgtacc tgagatggtg taataaattt tcgaaaaaac
      301 aggattgcca gcggtatccg ttttgatgac aaatacatca tgataatcct gaaatgaact
      361 tttgttgatg gtagtacctc cggtaatgac gtaatttttt tggtctttag tgacgagtac
      421 atcgtaaccg gtagttgact ggttggcagc accgtaggct tttaggaatt tggcaacacc
      481 ttctgcattg gtctttaaca gaaaagaata aaatgtaccg tcaacgatat aacgaccagt
      541 agtcagcaaa ttgccataag cgtcttctgc tataccataa gcctcatcga tattcttatt
      601 gttgccgtat gttttactcc ataatacttc acctgtagca tcaaatttta ccaaatatac
      661 atccgaccag ttattgagga catcagtacc ataactttta gtcatccctg ctacagcgaa
      721 accgccgtcc tgagtctcga cgagagcctg tccgcgggtt tctccaactg tcaccatcct
      781 tttcttccat tttacaaaac catcataacc aattctgatg acaatcatcg cgtaattgtt
      841 aaaactacct gtgttggata caacgactat gtcattttcg cgtgtatcaa ttaccctggg
      901 agaaacatta ttggccgtac ttgaagtacc gaactctctt acccattcca cttcaccatc
      961 acatttcagt tttgtaagtc ttacaaacga gccaccggta gtgtcggcga tgccaatcat
     1021 agcataccca ccgtctttca gggctgtaca attgagggtt tgtgtcggga aatctctttt
     1081 gtaaattttt tgaaatgtgt atttctgtgc gaaagattgt tggttaaaac cacataaaat
     1141 gatcgatatg caaattaatt tcaagaattg tttcatagta ttacccaaaa ttcagttatc
     1201 tttatttcaa agtataaagg tataaaaaat ctgattattt cgttccattg tgaatgggca
     1261 cttaaatttt caacgtatgg ttaatagaag aaagtttttc caaattgcag gaagtactgc
     1321 acttgccatg agtccggtga ccaggatgct atgcaaaaca gatgaaaggg ctgtccggca
     1381 aaatttcaat ctgcccgact actatcctat tgaagaatca attgaagatg aagatttttg
     1441 gtaccaggta cgtatggctt acacggtttt cccaaacctt ataaacctaa acaacggtgg
     1501 tgtgagtcca tcgccaaaga ttgtacaaga agcagtagag cgctacaaca aacttaccaa
     1561 tgaggctcct tcgtattaca tgtggcgcat acttgatcag ggacgagaac cattgcgcga
     1621 aaagctggct ttgcttgccg gttgtgatac agatgaaatc gccatcaacc gcaattcgtc
     1681 tgaagcactc gaaaccatca ttttcgggct caggcttaag caaggtgatg aggtggttct
     1741 taccaggcaa gactatccca acatgattaa tgcctggaaa caacgtgaaa aacgcgatgg
     1801 aatagtcctc aaatggatcg acctgccact gcctatggaa aatgacgatc aaattgtaca
     1861 aaaatattct gaacagtttt caacaaaaac caaaatcgta cacattaccc acattattaa
     1921 ctggaccggt cagatcattc ccgcaaggaa aatagcagat attgcccacc aaaaagggat
     1981 agatgtactt gtagatgcag cgcattcgtt tgtgcacatt ggttataaga taccagactt
     2041 agattgcgac tattttggca ccagcctgca caaatggctt tgcgcaccta tcgggtcagg
     2101 aatgctctat gtaaaaaagg aaaaaatcag ccaactctat ccactttttg ctgctggcga
     2161 cccagaaagt gccgatatac gaaaatttga aaacctcggc acacgctctt tcccgataga
     2221 gcaaggcatc agcgaggcca tcgattttca gaatatgctc ggtatggagc gaaaggaaaa
     2281 acggcttcgt taccttaaaa actactggac aacaaaagta caggatttgc cgggcgtcaa
     2341 attaaacacc tcattgaagg ataactacag ttgtgccata gccaatttta gcatcgaagg
     2401 caaaaaacct gaagaaatag aaaatacgct gttcagcaaa tacaagattc atacagtggc
     2461 cattaactgg gagaatatac acggtgtacg cgtgactcct aatgtatata cgctcacctc
     2521 agacctcgac aaattggtaa aaggtataag agagattatc aaatcctaaa acaacagctt
     2581 actttacttt tgcccaagta ttacctttct tagtacaaac gtatgcatct ttaaatcctg
     2641 ctttcatagc attttcgagt gatgccttag ctccggtcag gtctttgtag taaccaagat
     2701 atactctcgt ccattcgccg gcaggttcag ttaaaacaat gccgagtgct gcaagcttag
     2761 gatcatccga gaatttcggg tcgcgtactg ctgcaatccg gaccctgaaa ttatcttccg
     2821 gtttaggttc ttctttcact actgtagcag gcttcaccgc tatgggtttt gcttctgctt
     2881 gagctggttt tacatctacc gcttttgttt ctggaacttt tgtcacaact tttggttcag
     2941 atggttttac ttttgccggt ttgttttctt cgggtttggc tgctacaggc tcctcagcct
     3001 ttttaacaga aacatactct ttgtcttcaa ctatgaaaca accctttacg tcattttggg
     3061 taagcagacg gatggcagtc tgagcgtcgg ctttggtagc gaaattaccg atgcgtagct
     3121 ttgatttttg accgtcatct tcagttgtat aggcaatggc accttttacg atcttgttat
     3181 atttagcata atcgatcgga gtcgaatttt tcacaacacc tacctgcaca gcataaagcc
     3241 cctttgaccg gtaatttttg ttggcttcat ttttcaattg cttgaaatac gcctcagttg
     3301 gttcaagcgt aactatcatt tttatctttt cttctgactt agctgaagta ttaacgagtt
     3361 tcgattccga tttaaaatta tcagacattg ttttgaagaa atacattttg tccttggtta
     3421 ggttgatttt caattcacct gctgcatcag acaagagttt ttggacagac tcgcctgcct
     3481 gagtcgatat ct
//
LOCUS       DAOQQY010000286         3484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13379_length_3484_cov_5.249344, whole genome shotgun sequence.
ACCESSION   DAOQQY010000286 DAOQQY010000000
VERSION     DAOQQY010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13379_length_3484_cov_5.249344"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="PLE23_11240"
     CDS             complement(<1..821)
                     /locus_tag="PLE23_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83882.1"
                     /translation="MKKIIVPIMGMLLLCLELSAQNVQYKVNHAGAATTYTPQQVASD
                     FGPRMLSKKDGVLKYDWHGGVDYSAGAGDSDKGYTILALEDGTISYIHKLGRIGFKYI
                     RVDGAHHYGYGHIFTSKDGVQSSGDFKLVQLLVPHSTLYAIYNTQTQQLFHTCKNASY
                     VDKPCSEFKYIEGTDTIMATNQVVTGSEIGPVGTSIDGKKADGTPDPDAAVEAHLHLY
                     NFGTVNNNTASDVADGNTLNPLQFATHTAPAYTVNVYKNSGFTQEGIGLVYPGTG"
     gene            complement(852..1937)
                     /locus_tag="PLE23_11245"
     CDS             complement(852..1937)
                     /locus_tag="PLE23_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83883.1"
                     /translation="MRHKRNYIILLLLLVVYICRAQEKPQPLPVVPWSQAKKEYITDP
                     NGGKFRIIEANNKLTARYEGIEFLDASGRVTKSFNLRTAPFAPAFLKARERNDETTLR
                     MTETWIEAKNMTLAQKKSALHPKYADNPKAINGLSAVMTSMSILADRAGENNFSGCIV
                     IKYLVENFVKGGLYNINTRVVIIDGQGKTVDIIDHIPYGINELVLSDDLRYVCYNYAD
                     MENAKEDEDSTIPTGLRLYDRKTRKNVIDKYFGETGMGQFPENPFKIGDKIVLTCNGK
                     MLGKKGYTSVFILKPEENAIFRKDEKIYYTPEGYGYGGEIRKIEKDTIIFEKKPNGVT
                     EKYDPNKDFQKMTFEEFNNLKFSVRAE"
     gene            2267..2641
                     /locus_tag="PLE23_11250"
     CDS             2267..2641
                     /locus_tag="PLE23_11250"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMX83884.1"
                     /translation="MIHQTLKEVRKEKGMTLQEVSDRTNIPVRTIERIESGETRLDME
                     RLELLAQVYDMSPTEIMALDNKGIHFSVDNSTGGNNGVAYYCVFHENEKDMYEKLLRA
                     KDELIEAQRREIELLKQMKGGQ"
     gene            complement(2712..3365)
                     /locus_tag="PLE23_11255"
     CDS             complement(2712..3365)
                     /locus_tag="PLE23_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83885.1"
                     /translation="MNIDKVKLYISKGDEAYKVLEGLNYESRVDYGGRTDAPDHYPAV
                     NSANVLTKTGEWGTGAAPGVNGIVPKAYRSQPEDDFCFTDFVTRLHKAHVPGSAAQYT
                     SCPAEAKYPDGRYKLKARVISINGDSTDSAVSPFLLDNYKPYISQVSMSVKGKQCYDA
                     GWELKYDYCLVSVKNSETQILSDRAILNKLSAVGFVLPDSIRERFDFSKSQSEATRC"
BASE COUNT          893 a          784 c          718 g         1089 t
ORIGIN      
        1 ctgccggtac cgggatacac taagccgatg ccttcctgcg taaagccgga gtttttgtat
       61 acatttactg tataggcagg cgcagtatgg gtggcaaact gtaagggatt cagcgtattg
      121 ccatctgcaa catccgatgc agtattattg ttaactgtac cgaaattgta taagtgtaaa
      181 tgtgcttcta ccgctgcatc agggtcaggt gtgccgtctg ccttttttcc atctatactt
      241 gtgccgacag gccctatttc actacccgtg actacttgat tggtagccat tattgtatct
      301 gttccctcta tgtatttaaa ctctgaacaa ggtttatcta cataacttgc atttttgcaa
      361 gtatgaaaca gttgctgtgt ttgtgtatta tagattgcat ataaagtaga atgaggtact
      421 aataactgaa ccagcttaaa atcaccggag ctttgcacgc catcctttga ggtaaatatg
      481 tgcccgtaac catagtggtg tgcaccgtcg accctgatgt atttaaagcc aatcctgccc
      541 aatttatgaa tgtatgagat agttccatct tccaatgcca gtatcgtata tcctttgtca
      601 ctgtcacctg cacccgccga ataatccact ccgccgtgcc agtcatactt aagcacaccg
      661 tcttttttag acagcatccg cggcccgaag tcagaggcta cctgctgggg tgtgtaagtc
      721 gttgctgcac cggcgtgatt gaccttatac tgcacattct gggcagataa ttcaaggcat
      781 agcaatagca ttcccattat gggcacaatt atttttttca tcttaaaata ggtttattta
      841 ggctgtttgt attattcggc tcgtactgag aatttcaggt tattaaattc ttcaaaggtc
      901 atcttttgaa aatctttatt ggggtcatat ttttctgtaa ctccgttagg ttttttttca
      961 aatatgatgg tatctttttc gatcttacga atttctcccc catatccgta tccttcaggt
     1021 gtgtaatata ttttttcatc tttcctgaat attgcatttt cctcaggctt tagaataaaa
     1081 acactggtat atcctttttt acctagcatt ttcccattac atgttaatac tattttgtct
     1141 ccgattttaa atggattttc cggaaactgg cccatgcctg tttctccaaa atacttgtct
     1201 atgacatttt tcctggtttt ccggtcatac aggcgaagcc ctgtggggat ggtactatcc
     1261 tcatcttctt tcgcattttc catatctgca taattatagc atacataccg gaggtcatca
     1321 gacagtacaa gttcattaat tccatacggt atatgatcta tgatatctac tgtcttgccc
     1381 tgaccgtcaa tgatgactac acgcgtattg atattataca gccccccttt tacaaaattt
     1441 tcaaccaggt actttatcac aatgcatccg gagaagttgt tttctcctgc acgatccgca
     1501 agaatgctca tactcgtcat caccgccgaa aggccgttaa tcgcttttgg gttatcggca
     1561 tattttgggt gcagagctga tttcttttgt gcaagagtca tgtttttagc ctctatccag
     1621 gtctcagtca ttctcaatgt cgtttcgtca ttacgttccc tggccttcag aaaggcagga
     1681 gcgaaaggag ccgttcgcag gttaaaggat ttcgtgaccc tgcctgaagc atccaagaac
     1741 tctataccct cataccgggc ggtcagcttg ttgttggctt caataatccg gaattttcct
     1801 ccattagggt ctgtgatgta ctccttcttc gcctggctcc acggaacgac aggcaggggc
     1861 tgaggtttct cctgtgcccg gcagatataa actaccagca gtaaaagcag tataatatag
     1921 tttcttttat ggcgcatgat tccgcatttt gatttaaaaa ttcttagtat atcattcata
     1981 aacagtaact ttacaataaa ggtaaagtat tctgctgata gtttttcaca tcgttggctg
     2041 gatttagctg atataaggca tgctgcaaat caacatatat catatcagca atatcctctg
     2101 attacctacc acaaatatat tacatctcaa aatttattta cctaccaatt tttggtgttt
     2161 atctaccatt ttttgtcgga ttttacggta ttttccgcca gatttggcgg attccggaaa
     2221 taattatgta tttttacatc catttaccgg ttaaaattat gctctcatga ttcaccagac
     2281 attgaaagaa gtccgaaaag aaaaagggat gacccttcag gaagtttcag acagaacaaa
     2341 tatacctgtc agaacgatag aacgcattga gtcaggagag accaggttgg atatggaacg
     2401 gctggaactg ttggcacagg tctatgacat gtctccgact gaaattatgg cactggacaa
     2461 taaagggatt cattttagtg tggataacag tacaggtggc aacaatggag tagcttacta
     2521 ttgtgtgttt catgaaaacg aaaaagacat gtacgaaaaa ctcctccggg ccaaggatga
     2581 gctgatagaa gcacagaggc gcgaaataga gctgttgaaa cagatgaagg gcggtcagta
     2641 atgaaggagg cagatttttt attacatttg ggcaaaagaa ttaaatccct tcggttagaa
     2701 aaagggctga ctcaacagcg agtagcttct gattgtgatt ttgaaaaatc aaacctttca
     2761 cgtattgagt caggtaaaac aaatcccaca gcactgagct tgttcaaaat cgcccggtca
     2821 cttaatatct gtgtctccga attttttacc gatactaaac aataatcata cttcaactcc
     2881 catcccgcat catagcattg cttccctttt acactcatac tcacctgcga tatgtatggc
     2941 ttgtagttgt ctaacaggaa gggtgacacc gcggagtccg tagaatcgcc attgatgctt
     3001 atcacccgtg ctttgagctt atacctacca tcaggatact tcgcttccgc gggacagctc
     3061 gtgtattgtg ccgcgctgcc gggtacatgg gccttgtgca gacgggttac aaaatccgtg
     3121 aaacagaaat cgtcttccgg ttgtgaacgg tatgccttgg gtacgatgcc gttcacgccc
     3181 ggagcggcac cggtgcccca ctccccggtt ttggtgagca cattagctga attcacagca
     3241 gggtaatgat caggcgcgtc tgtccggcct ccgtagtcta cccggctttc gtagttaagc
     3301 ccttctaaca ctttgtatgc ttcgtctccc ttgctgatgt acagctttac tttatctata
     3361 ttcatcaccg cgctgtagtt gccgccgccg ttttggttgg ccatacgcac acgtacgcct
     3421 gctttagtgc tgccggtacc gggatacact aagccgatgc cttcctgcgt aaagccggag
     3481 tttt
//
LOCUS       DAOQQY010000287         3468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13492_length_3468_cov_5.335775, whole genome shotgun sequence.
ACCESSION   DAOQQY010000287 DAOQQY010000000
VERSION     DAOQQY010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13492_length_3468_cov_5.335775"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLE23_11260"
     CDS             complement(<1..383)
                     /locus_tag="PLE23_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMX83886.1"
                     /translation="MSEKILYLLDGHALVYRAHYAFISRPLINSKGQNVSAVSGFMRT
                     VWDLIKNRKPTHIAAAFDLYSDTFRHKMYEPYKANRDAQPEDITYAVPKVIDILKAMN
                     IPIMAVENYEADDVIGTIACKAENEG"
     gene            complement(703..1677)
                     /locus_tag="PLE23_11265"
     CDS             complement(703..1677)
                     /locus_tag="PLE23_11265"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HMX83887.1"
                     /translation="MKLSFTIFFAFILSGLSMAQAGNDRLTEVKKLSAKEGDGWVKGA
                     GIGLDFTQLALINPRFGSGENKIGFGGLGNLFANYKRDKIYWNNAASMQFAVQQIGIS
                     SNPFLKNLDRFVFGSKIGYKFNDKWAAALDANLETQLTPTYQGNILKPTKGESIVANF
                     FAPATIKVSPGIDYLATPHLTFFFSPASLKLIYINDQKIADLGIFGTKANDNGIGFKK
                     SDLQLGANLKGIYTNKLFNDKLMVNSVLDLYSNYLRNPQNIDVNWHNDFSWNIYKGLA
                     LNLLVDVLYDDDILVQVDRNKNKIYEPGEEGKRPTIIQALLIKYNLVF"
     gene            complement(1703..2509)
                     /locus_tag="PLE23_11270"
     CDS             complement(1703..2509)
                     /locus_tag="PLE23_11270"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMX83888.1"
                     /translation="MEILHELALKFAQTIPNLTGAFLLIIAGIILSKLVSKFMQKMLK
                     ALNIDRFGEKLNDIDLVRKSGIDIKISKVVSKVTYYFLLLIFIVAATDVLNMPVISNL
                     VSDLINYIPNVIVALFILIIGLIVAENIRVVVLTVCKSLGISSAKTISSALFYFLFLT
                     VLLSALEQARIQTSFLANNLTVMIGGIVLAFALGYGIASKDIMTNFIVSLLQKHKFKK
                     GDHIKVGNHSGIVTEMDNNSVTLTTDEKQIIIPLNKLSTAEVEIFLAKKS"
     gene            complement(2522..2740)
                     /locus_tag="PLE23_11275"
     CDS             complement(2522..2740)
                     /locus_tag="PLE23_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83889.1"
                     /translation="MENHDVNEQAQPDEIKIPVNLEKYLQDNLKVFKFAGDVVNLYFT
                     GLVRTGMDMITPTTYEFDKREKETDKED"
     gene            complement(2722..3318)
                     /locus_tag="PLE23_11280"
     CDS             complement(2722..3318)
                     /locus_tag="PLE23_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMX83890.1"
                     /translation="MNQKKLKIPDAELVRLYIDTQSQEYFSMIYNRYANKVYAKCLSM
                     LKNEEWAIDASQDIFMKIFMNLVRFEEKSQFSTWVYSITFNYCIDSLRKHKKENEIFA
                     HEKELKDQPYEDDNEAEVFEMEAQKLEKVMDMIPGEDKALLIMKYEQSMSIREISEIL
                     DKTESAVKMKIKRAKEKAKILSNMIMNVLVLFLWKIMM"
BASE COUNT          938 a          638 c          703 g         1189 t
ORIGIN      
        1 ccttcgtttt cggctttaca agcgatggtt ccgatgacgt catctgcttc gtaattttct
       61 actgccatga tgggtatgtt cattgccttg aggatatcga ttacctttgg tacggcatag
      121 gtaatatctt cgggttgggc atcgcggttg gccttgtaag gttcgtacat tttgtgtctg
      181 aaagtgtcag aatacaggtc gaatgctgca gcgatgtgag ttggtttcct gtttttgatg
      241 aggtcccata cggtacgcat aaatccgctt actgcagata cgttttgtcc ttttgagttg
      301 atgagtggtc tggagataaa agcataatga gccctgtaaa cgagtgcatg gccatccaac
      361 agatacagta ttttttctga cattgccgta atttttgaaa ggtgaagtta ctgatattgt
      421 cagttatatt aagagaccaa gcaaaatttg tgcttaaaat aattcaaccc agataaagca
      481 atagcctctt tactgcaaat aatgattact tcatatccat tttaaaagct caaaaaccat
      541 agttcactat gctattataa cttttttatg gattttttgt actaatcatt ttcgtttagt
      601 catactgcgt tatctgggtt caaacagttg attcattcaa taaaaaaagc tccggattgg
      661 cagtatccgg agcttttttc atctattggc taataaacag tatcagaaca caaggttgta
      721 cttgataagc agtgcctgaa tgatagtagg gcgtttacct tcttcacccg gttcatagat
      781 tttgtttttg tttctatcca cttgtacgag tatatcatca tcatataata catcaaccag
      841 caagttaagt gcaaggcctt tgtagatatt ccagctaaag tcattatgcc agttgacgtc
      901 aatgttttgt ggatttctaa gatagttaga atacagatcg agtacagagt tgaccatcag
      961 tttgtcatta aatagtttgt ttgtatagat gcctttcaga ttggcaccga gctggaggtc
     1021 agatttttta aatccgatac cattatcatt agcttttgtg ccaaaaatac cgaggtcggc
     1081 aattttttga tcgttgatgt agatcaattt caatgaagca ggtgagaaga agaatgtgag
     1141 atgtggagta gccaggtaat cgatacccgg agataccttt atggtagccg gagcaaagaa
     1201 gttggcgacg atactttccc ctttggttgg ttttaggata ttgccctgat aagtaggtgt
     1261 aagttgtgtt tcgaggttgg cgtcgagtgc tgctgcccat ttgtcattga atttgtatcc
     1321 gatttttgat ccgaatacaa atctgtcgag gtttttgagg aatgggttgg acgagatgcc
     1381 aatctgctga acggcaaact gcattgaagc tgcattgttc cagtatattt tatctctttt
     1441 gtagttggca aacagattgc ccaagcctcc gaagcctatt ttgttttcac cggaaccaaa
     1501 acgcgggttg atgagggcca attgtgtaaa atcgagcccg ataccggcac ctttgaccca
     1561 gccgtctcct tctttggcag acaatttttt aacttctgtc aggcggtcat tgcccgcttg
     1621 tgccatggac aaacctgata agatgaacgc gaaaaagatt gtgaagctta gtttcataca
     1681 gttaagtttt taagttattt ggttatgatt tttttgctaa aaatatttca acctcagcgg
     1741 ttgacaattt gttgagcgga atgataatct gcttctcgtc ggtggtaagt gtgacagaat
     1801 tgttgtccat ctctgttact ataccggaat gattacctac cttaatgtgg tccccttttt
     1861 tgaatttgtg cttttgcagc aatgaaacta tgaaattggt cataatatct ttggatgcaa
     1921 tgccatatcc gagtgcaaat gcgagtacta taccgccaat cattacagtc agattgttag
     1981 caaggaaaga agtctggatg cgtgcctgct cgagtgcgct gagcaataca gtcaggaaaa
     2041 ggaagtaaaa tagtgctgaa gaaatagttt ttgcagaaga gatgccgagc gatttacaaa
     2101 ccgtcagaac tacgactctt atattctctg ccacgatgag gccgataata agaataaata
     2161 gtgcaacaat cacattcggt atataattga tcaggtcgct taccagattt gagatgaccg
     2221 gcatgttgag tacatccgta gcggctacta taaatataag caacaagaag tagtaggtta
     2281 ctttagagac aactttgctg atttttatgt caatacctga ttttcgtacc aggtcgatgt
     2341 cattgagttt ttccccaaag cgatcgatat tgagtgcctt gagcattttt tgcatgaatt
     2401 tggagaccaa ctttgacaga ataattcctg caatgatcaa caggaatgcc ccagtgagat
     2461 taggaatggt ttgggcgaat tttaaggcta attcgtggag aatttccata agcttgtttt
     2521 tttagtcttc tttgtctgtt tctttttctc ttttgtcgaa ttcgtaagta gtaggtgtta
     2581 tcatatccat gccggtacgt accaatccgg taaagtacag gttgacaaca tcacctgcaa
     2641 atttgaaaac cttgagattg tcttgcaggt atttttcaag attgaccggt atttttattt
     2701 catccggttg tgcttgttcg tttacatcat gattttccat aaaaataata ctaacacgtt
     2761 cataatcata ttgctcagga tttttgcttt ttctttagct ctttttattt tcattttaac
     2821 ggcactctct gttttgtcga gtatttcgga tatttctctg atagacatac tctgttcgta
     2881 tttcataata agcagagctt tgtcttcacc cggtatcata tccattacct tttccagctt
     2941 ctgtgcttcc atttcgaaaa cttctgcttc gttatcatct tcgtatggct ggtctttcaa
     3001 ttctttttcg tgagcgaata tttcgttttc ttttttatgc ttccggagtg aatcgataca
     3061 ataattgaat gtaatcgaat aaacccatgt actgaactgc gatttttctt caaacctaac
     3121 caggttcatg aatattttca taaaaatatc ctgactcgca tctattgccc attcttcatt
     3181 tttgagcata gagaggcact tggcatacac tttattggca tacctgttat atatcatgct
     3241 gaaatattcc tgactttggg tgtctatata cagccgtacc agttcagcat ccggaatttt
     3301 tagttttttt tgattcaatt aatctccggg gttacagatt attcaattgg ttcacgggtt
     3361 taagcttatg acgcaaatat ccgaaaaagt tacatcacag accaatgaaa cccataattt
     3421 cattgaatgc cttcaaaagt ataaaagctt agtacgatta attcactt
//
LOCUS       DAOQQY010000288         3459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13540_length_3459_cov_4.467685, whole genome shotgun sequence.
ACCESSION   DAOQQY010000288 DAOQQY010000000
VERSION     DAOQQY010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13540_length_3459_cov_4.467685"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLE23_11285"
     CDS             complement(<1..926)
                     /locus_tag="PLE23_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMX83891.1"
                     /translation="MKNIRNFCVIAHIDHGKSTLSDRLLEYTKTISERNMQDQALDDM
                     DLERERGITIKSHAIQMYYHRNGERYTLNMIDTPGHVDFSYEVSRSIAACEGALLLVD
                     ATQGIQAQTISNLYLAIEHNLEIIPVLNKIDMDNAMIEEVSDQIIDLIGCKKEEILHA
                     SGKTGAGVNELLEAIVERIPSPKGEEEAPLQALIFDSVFNPFRGVIAYFRIINGTLRK
                     NQKVRFFYTGKEYNADEIGILQLNQVPKDELKAGDVGYIITGIKVSKEIKVGDTITSV
                     DNPCAEGIQGFEDVKPMVFAGIYPIDNDDFED"
     gene            1159..1605
                     /locus_tag="PLE23_11290"
     CDS             1159..1605
                     /locus_tag="PLE23_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83892.1"
                     /translation="MKKLLLLFAITVLSTTAAFAQIEKIINQDFNIDQFNTVQFELSK
                     DYYLEKWAGTTILVETKVKLTGASPAILKFAIESGRYEILNIEDGDKVTLKSKLSKPP
                     LLKNKSVDIEEVVEVRIMVPEDFNTEDFSVITREKDTNSSTIRNED"
     gene            1788..3452
                     /locus_tag="PLE23_11295"
     CDS             1788..3452
                     /locus_tag="PLE23_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HMX83893.1"
                     /translation="MYSSINSQGVLEDIYQDLYRILYLCCFLKIEARILMYQQVEIKV
                     APEFVSDEAYQFSLIGDSLGMNPSAFSARLVKRSIDARRFPAHYLLRFDVFTDMVPDP
                     EPALLSEFKDVSEAPEVLIIGAGPAGYFAALDLIALGLKPVIFDRGKDAQTRRRDLRA
                     IQQFGIVNPDSNYCFGEGGAGTYSDGKLYTRSNKRGEILRPLKLLVEHGASPDILVDA
                     HPHIGSNKLPKVIANIRETILHHGGQVHFDAKLTDIILRDGEVKAVRINDKKEYKCDT
                     LILATGHSARDIFYLLHQKDILIEAKAFALGVRIEHPQGLIDKIQYKQSPRSEHLPAS
                     SYKLVTQVGEKGVFSFCMCPGGLIVPSATSPGELVMNGMSMSRRDSPFANSGTVVTVS
                     EGEVKGYDDAGPLKLLEYQKMVEQSLFDKGDGSQKAPGQRLTDFFNRKISASIQENSY
                     IPGVYSAPLHKWLPTMIYEPLRQAVKDFGRKMNGYFTEEALVVATESRTSSPVRIPRD
                     KESLMHPQVRGLFPCGEGAGYAGGIISAAMDGEAVAKAVANYKTTR"
BASE COUNT          993 a          718 c          713 g         1035 t
ORIGIN      
        1 aaatcttcaa aatcatcatt atcaataggg taaatacctg caaataccat cggtttaaca
       61 tcttcaaagc cctggatgcc ttctgcacac ggattatcga cacttgtgat ggtgtcaccc
      121 acttttattt cttttgatac tttaataccg gtgatgatat acccgacatc ccctgccttg
      181 agttcgtctt tgggtacctg attgagctgg aggatgccta tttcatctgc attgtattcc
      241 ttgccggtat agaagaaacg tactttctgg tttttacgca gtgtaccatt tattattcta
      301 aaataagcaa taacgcctct gaacggatta aatacagaat caaaaatcag agcctgcaat
      361 ggagcttcct cttcgccttt aggggaaggt attctttcta caatagcttc gagcagttca
      421 ttcactccgg caccggtctt accacttgcg tgtagtattt cttctttttt acagccaatg
      481 aggtcgataa tctggtcaga tacttcttct atcatggcat tgtccatgtc tattttgttg
      541 agtaccggta ttatttccag attgtgttcg atggcaaggt aaaggttgga aatggtctgt
      601 gcctgtatgc cctgggtggc atccacaagg agtaatgcac cttcgcaggc tgcaatagaa
      661 cgtgagactt cgtatgaaaa gtcaacgtgt ccgggtgtat ctatcatgtt caaggtatat
      721 ctctcaccat ttcggtgata atacatttga atggcatgac tcttaatggt gataccgcgt
      781 tcgcgctcca agtccatgtc gtccaatgcc tggtcctgca tattgcgttc agagatggtc
      841 ttggtatatt ccagcaaacg gtcagaaagt gtacttttgc cgtgatctat atgcgctata
      901 acacaaaaat ttctaatatt tttcataggg cgcaaatata cggctttttc agattcaaaa
      961 ccaccaggaa actattagtt tgatcataat attattgacg ccaattggaa atggtattgt
     1021 tttttgccgg aatatacagt ttcactcaat aacaattatt tcacttgatg cctcgatgtc
     1081 tgcatggtga aattataatc aaacgttaaa tctttagttt tgagtgacga attgattaca
     1141 tttgcgtttt acaagtatat gaagaagtta ctcttattat ttgcaattac agttttgagc
     1201 acaactgccg catttgctca gattgaaaaa atcatcaatc aagattttaa tatcgatcaa
     1261 ttcaatacag ttcaattcga actttcaaag gattattatc ttgaaaaatg ggcaggtaca
     1321 accatcctcg tcgaaactaa agttaagctt accggtgcat caccggctat tctcaagttt
     1381 gccatagaat ctggtcgtta cgaaatacta aatatagaag acggcgacaa agtgacattg
     1441 aaatcaaaac tcagcaaacc accacttctc aaaaacaaat cggttgatat tgaagaagtt
     1501 gttgaagtga ggattatggt accggaagat ttcaatactg aagatttttc ggttataaca
     1561 cgtgagaaag ataccaattc ttctactatc cgcaatgaag attgaagtgc tacgatagac
     1621 actcttaagc ccctgtagaa attttcactt ttgccttcgc tctcaacgtg ttaagtgagg
     1681 tattttatgt caactttgcc ggtattttca ttgcaccaaa catgctgatc tatattttaa
     1741 tataaaatct gaaggcttaa taatttattc gttcttaata aataataatg tacagctcta
     1801 ttaattcaca gggtgtactt gaagatattt accaagattt ataccgaata ctttaccttt
     1861 gttgttttct taaaatagaa gcccgtattc ttatgtacca gcaggttgaa ataaaagtcg
     1921 cacccgaatt tgtttctgat gaggcttatc agttttcgct tattggtgat tcattaggta
     1981 tgaatccgtc tgcattttcg gccaggctgg taaagcgttc gattgacgca cgtaggtttc
     2041 cggctcatta tttgcttcgt ttcgatgtat ttaccgatat ggtacctgac ccggaaccag
     2101 ctttactttc agaatttaaa gatgtaagtg aagctcctga agtgcttatc atcggggccg
     2161 ggcctgccgg ttattttgct gcccttgacc ttatagcact cggcctcaaa ccggttattt
     2221 tcgatcgagg gaaagatgcc caaacccgac gtcgcgacct cagagccatc cagcagttcg
     2281 gcattgttaa tccggattcg aactattgtt ttggtgaagg tggcgcaggt acttattctg
     2341 acggtaaact atatacacgc tccaacaagc gaggcgaaat acttcgtccg cttaagctgc
     2401 tggtagagca cggcgcatct ccggacatac ttgtagatgc acatccgcat atcggatcca
     2461 ataaactacc caaagtcata gccaacatcc gtgaaaccat ccttcatcat ggcggtcagg
     2521 tacattttga tgctaaactt acggacatta tcctccgtga tggcgaagta aaggctgtgc
     2581 gcatcaatga caaaaaggag tacaaatgtg ataccttaat ccttgcaaca ggccactcgg
     2641 cacgcgacat attttacttg cttcatcaaa aagatatttt aatagaagcc aaagcatttg
     2701 cacttggtgt acggatagaa catccacaag gcctcatcga caaaattcag tacaagcaat
     2761 caccccggag cgaacatttg cctgcttctt cctataaact agtaacgcag gtaggtgaga
     2821 aaggcgtttt ttcgttttgt atgtgtccgg gcgggttgat cgtgccctcc gctacttcac
     2881 cgggcgaact agtcatgaat ggtatgtcca tgtctcgtcg cgattcccct tttgccaaca
     2941 gtggaaccgt cgtaactgtc tcggaaggag aagtaaaagg atatgatgac gcaggcccgt
     3001 taaaacttct tgaatatcag aaaatggtcg aacagagttt gttcgataaa ggtgatggta
     3061 gccaaaaagc acccggtcaa aggcttacag attttttcaa cagaaaaatt tctgctagca
     3121 tacaggaaaa ctcctatata cccggtgttt acagtgcccc cttgcataaa tggctgccaa
     3181 cgatgattta tgaaccactc agacaggcag taaaggattt tggaagaaaa atgaatggtt
     3241 attttacaga agaagcgctt gtggtagcta ccgagagccg taccagttcg ccagtgcgca
     3301 taccacgtga taaagaaagc ctgatgcacc cacaagtgag aggcttgttt ccttgtggag
     3361 aaggtgcagg ttatgcagga ggaattattt cagcagcaat ggatggcgag gctgttgcca
     3421 aagctgttgc caattataaa acaacaagat aaaatgaaa
//
LOCUS       DAOQQY010000289         3448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13602_length_3448_cov_5.641910, whole genome shotgun sequence.
ACCESSION   DAOQQY010000289 DAOQQY010000000
VERSION     DAOQQY010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13602_length_3448_cov_5.641910"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="PLE23_11300"
     CDS             <1..547
                     /locus_tag="PLE23_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83894.1"
                     /translation="KDEINASFSKIEVFNPNKSVAWGGIYWQYFEQMDKIKTFKETPL
                     KIVKNYFIVKNTDAGEVMTPVSASNQVKLGDKVRVRIEIRVDRMMEYVHLKDMRGSGF
                     EPVNVLSQYKYKSGLGYYESTGDAATNFFISYLPKGTYVFEYDLRANNKGDFSMGIST
                     MQCMYAPEFSSHSGGERIGIK"
     gene            565..1032
                     /locus_tag="PLE23_11305"
     CDS             565..1032
                     /locus_tag="PLE23_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HMX83895.1"
                     /translation="MEIERKFLVNKSAWEIAQQGESARICQAYLANDEHKVVRIRTYG
                     NQAFITIKAHLAGMSRAEYEYPMPLQDADEMIVTLADKRIEKIRHRVNYKGKLWEVDV
                     FEGENEGLLVAEIELEYENEAFEKPEWVGEEVTDDLRYYNASLIQNPYRNWNR"
     gene            complement(1260..1649)
                     /locus_tag="PLE23_11310"
     CDS             complement(1260..1649)
                     /locus_tag="PLE23_11310"
                     /inference="COORDINATES: protein motif:HMM:NF022264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HMX83896.1"
                     /translation="MIKQAFLISTLIMVGIALSSFDMSKWRIIGQKEAAYGPDRDVLF
                     VKGNDIFSAVKIKVVDAGLNMKDMGIHFENGQIQHVPLQKNFHQGEESRVIDLPGNKR
                     RIEKITFLYDTKGVMKGKANVIVFGRR"
     gene            complement(1919..>3448)
                     /locus_tag="PLE23_11315"
     CDS             complement(1919..>3448)
                     /locus_tag="PLE23_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HMX83897.1"
                     /translation="NANEWLKAMEKADKTTNAGNEPDGSKANQEVFDRFDFALNANIG
                     KLLFNEYELNNIITDGWMTPNKLQLRKLSFVMGQSDFSASGSVNNIFNWLFKNQNLGG
                     DIVFSSHNLDMNQFMQADPKAKTTTATEPLIIPANIQMNIKTDIGKLSYTNMTLNNVS
                     GTLLVADQAVKIIGGTANTLGGKVNISGGYNSKDSSKPGFDIKLGLEKIGFTDAFNTF
                     NTIKKLAPIAQYITGAFNTELSMSGSLTKDMSPDLNTLQADGFLETLSGVIKNFKPLN
                     DVGNKLNIKEFNNFELRNTKNWITIKNGAVEVKDFDYSFKNIAMQIGGKHGLNQDMDY
                     KIKAKIPRKMLESNTVGAAAYSGIGFLSKEASKYGVNISAGEFVNVLIGIGGSMLSPK
                     LNFKILGTEGASVKNQVSETVGSAITNVKDSINRRAQQEVQKVKDKAKAEADRMADSL
                     AKVANQKADEAIRKAQEELQNKIGKEVSDKVGDKVGDKAKSEIEKAKDKLKKYDPFKK
                     K"
BASE COUNT          988 a          652 c          679 g         1129 t
ORIGIN      
        1 aaaggacgaa atcaatgctt cattcagtaa aatcgaagta tttaatccga ataagagtgt
       61 agcttggggt ggcatttact ggcagtattt cgaacagatg gacaagatta agactttcaa
      121 agaaacaccg ctaaaaatcg ttaaaaacta tttcatcgta aaaaataccg atgccggaga
      181 agtgatgaca ccggtttctg cttccaatca ggtaaaactc ggtgacaaag ttcgagtaag
      241 aatcgaaatc agagttgacc gtatgatgga gtatgttcac ctgaaagata tgcgtggaag
      301 cggattcgaa cctgtgaatg tactaagcca gtacaaatac aaatcaggcc tgggatacta
      361 cgaaagtacg ggagatgcag caaccaattt tttcattagc tatcttccga aaggaaccta
      421 tgtttttgag tatgatttgc gtgctaataa caaaggcgat ttttcgatgg gtatcagtac
      481 gatgcagtgt atgtatgctc cggaatttag ttcgcattcc ggtggtgaaa ggattggaat
      541 caaataaatg ctctaataat gggaatggag atcgaaagaa aatttctggt aaacaaatct
      601 gcatgggaga tagcccaaca gggagaaagt gccagaatat gccaagctta cttagccaat
      661 gacgaacata aagttgtgag aataaggacc tatggcaacc aagcattcat aaccattaaa
      721 gcacatttag ccggtatgtc aagagctgaa tatgaatacc cgatgccact acaggatgct
      781 gacgaaatga tagtcacact tgccgacaag agaattgaga aaattcgcca tcgagtcaat
      841 tataaaggaa aactttggga ggtagatgtg tttgaaggtg aaaatgaagg acttttggta
      901 gccgaaatcg agttggagta tgaaaacgaa gccttcgaaa aaccagaatg ggtaggagag
      961 gaagttacag atgacctcag atattacaat gcctctctca ttcaaaaccc ataccggaac
     1021 tggaatcgtt gatttattaa cccaaataag gcattaggga ttaaaacaaa aatgattcgt
     1081 acaaaaaatc cataaaaaaa gagttaaatg catattgacc tatggatttt aaacttttga
     1141 aatggatttg aagtaatatt tatttgcagt aaagaggcta ttgcgtgatt cgggattaat
     1201 agaaaagctg ccctgaaatg ttatcggggt agctttttcc ggtggtaaac aatgatgttt
     1261 tatcgccttc caaatacaat tacgttggct ttacctttca taacgccttt ggtatcataa
     1321 aggaaagtaa tcttttcgat acggcgtttg ttgcctggca gatcgatcac acgactttct
     1381 tcaccctggt gaaaattttt ctgtagtggt acatgttgta tctgaccatt ttcaaaatga
     1441 atgcccatgt ctttcatgtt aagtccggca tcaacaacct ttattttgac tgcagaaaaa
     1501 atgtcatttc ctttgacaaa taatacatcg cggtcagggc cgtatgccgc ttccttttga
     1561 ccaataattc tccactttga catatcaaat gaactaagtg cgatacccac cataatgagc
     1621 gtgctgatta agaatgcttg ctttatcata attattgatt ttattacttc attgacagga
     1681 atcatgactc agggtttaat cagtgtgcaa attttttaca ttttttttgc ctaaatattt
     1741 cttaaaaaat ttagtaatat gttttagcat gaattatagt aaagcattgg atatattagg
     1801 tgacagatga ttctagtaaa accataacca attgatatct gccttttatg aacaaatgca
     1861 aaagccaata gggtcttatt ctactggctt tcgtgtttgg taggtatgtg gaaatatctt
     1921 acttcttctt gaaaggatcg tactttttca gcttatcttt ggctttctct atttcggact
     1981 ttgctttgtc accgaccttg tcgccaactt tatcactaac ttccttcccg attttatttt
     2041 gtagttcttc ttgtgctttt ctgatagctt catcagcttt ttggttggcc acctttgcca
     2101 gcgaatcggc cattcggtct gcctctgctt tggctttatc ctttactttc tgtacttctt
     2161 gttgtgccct gcggtttatt gaatctttga cattggttat ggccgaaccc accgtttctg
     2221 aaacctgatt tttgaccgaa gctccttcag taccgagaat tttaaagttt aactttggtg
     2281 acaacatgct tccaccgatt ccaatcagta catttacaaa ttctccggct gagatattta
     2341 ctccgtattt tgaagcttcc ttactcagaa aaccaatgcc tgaatatgct gcggcaccta
     2401 ctgtattact ctcaagcatc tttcttggta ttttggcctt aattttatag tccatatctt
     2461 ggtttaggcc gtgttttcca cctatttgca tggcaatatt cttaaagcta taatcaaaat
     2521 cttttacctc aacagcaccg ttcttgattg ttatccagtt tttagtgttt ctcagttcga
     2581 agttattaaa ttccttaatg ttaagtttgt tgcctacatc attcaatggt ttgaaattct
     2641 tgataactcc actcagtgtt tcaaggaatc catctgcctg taaagtattc aaatccggac
     2701 tcatgtcttt tgtgagacta cccgacatgc tcagttctgt attgaaagca cctgtaatat
     2761 actgtgcaat gggtgcgagt ttttttatgg tgttgaaggt gttgaaagca tctgtaaatc
     2821 cgattttttc aaggccgagc ttgatgtcaa atcctggttt agatgaatct ttgctgttat
     2881 atcctccgga aatgttcacc ttaccaccaa gtgtgttggc agtaccaccg attattttta
     2941 ctgcctggtc agcaaccagc aatgtgccac tcacattgtt aagtgtcata ttggtgtagc
     3001 tgagtttgcc gatatcggtc ttgatgttca tctgaatgtt ggccggaatg ataagtggct
     3061 cagtggcagt ggttgttttt gctttcgggt cggcctgcat aaactggttc atatcaagat
     3121 tgtgtgagct gaagacgata tcaccaccga gattctgatt tttgaataac cagttgaaaa
     3181 tattatttac acttccgctc gcactaaaat cactttgacc cattacaaac gatagttttc
     3241 taagctgaag tttattgggt gtcatccatc catcagtaat aatattattg agttcatatt
     3301 cattgaacaa aagtttgcca atgttggcat ttagagcaaa atcgaacctg tcgaagactt
     3361 cctgattcgc tttgcttcca tccggttcat tacctgcgtt tgttgtcttg tctgccttct
     3421 ccattgcttt gagccattca ttagcatt
//
LOCUS       DAOQQY010000290         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13631_length_3444_cov_5.165536, whole genome shotgun sequence.
ACCESSION   DAOQQY010000290 DAOQQY010000000
VERSION     DAOQQY010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13631_length_3444_cov_5.165536"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..813)
                     /gene="murQ"
                     /locus_tag="PLE23_11320"
     CDS             complement(13..813)
                     /gene="murQ"
                     /locus_tag="PLE23_11320"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HMX83898.1"
                     /translation="MQLTTESSSEYRHLEKMPVNELLLGINDEDSKVAAAVRKSIPQI
                     HSLITALVGFMKEGGRLFYIGAGTSGRLGILDASECPPTFGVDHGLVIGIIAGGDSAI
                     RKAVEFAEDDTDLAWKMLSGYNISTKDFVIGIAASGTTPYVIGGLEKCRQQGIATGCI
                     TCNASSPVAQVSDYPIEVVVGPEFLTGSTRMKSGTAQKMVLNMISTVAMIQLGHVADN
                     KMVDMQLSNHKLINRGVKMIMEKKSISFEQAKELLLKYGSVRAVLDKQ"
     gene            complement(821..1105)
                     /gene="yajC"
                     /locus_tag="PLE23_11325"
     CDS             complement(821..1105)
                     /gene="yajC"
                     /locus_tag="PLE23_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMX83899.1"
                     /translation="MEALGQLLPFLLLFVVFYFFFIRPQSKKQKEQNMFVANLQKGDE
                     VVTMSGIIGRITKIDEQSVQLEIEGKTYLKFLKSTISKEMTDAYVKSSAA"
     gene            complement(1135..1614)
                     /locus_tag="PLE23_11330"
     CDS             complement(1135..1614)
                     /locus_tag="PLE23_11330"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMX83900.1"
                     /translation="MNKMLITIIFLTAAFFLNACKHSDADEVVEIRPSKPGSVAELIK
                     SPVTAEGIKDTSKMAKIQFEETSFDFGTIHQGQVITHFFKLKNVGKTPLIISDGHSTC
                     GCTVPEWPKTPIAPGDTASIKVVFNSEGKKDAQNKPVFIYANTLPAENKIYITGIVK"
     gene            1654..2262
                     /locus_tag="PLE23_11335"
     CDS             1654..2262
                     /locus_tag="PLE23_11335"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMX83901.1"
                     /translation="MLEKTDDQMDINDIQMIFIIGFMGSGKTTIGRLLANRLGWNFID
                     LDTLIQIREGRSIPDIFESHSEAFFRKAEYNALRQLGGLKNIVVSTGGGTPCYFDNIH
                     FMKSTGKVIWLDNDFGTLYKRIIGTQSGTRPLVSGNSKEKLKELFVTRGEYYKLADVK
                     ISCEPDDLPEAILAKIVKFFLWHTPIYTVTKIIIQVFLSELL"
     gene            2310..2825
                     /locus_tag="PLE23_11340"
     CDS             2310..2825
                     /locus_tag="PLE23_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83902.1"
                     /translation="MKKFGFIVDLSDTAQEKYEQIVDAFDRFSDELSPKHLKNTQLRI
                     ILPPGSPYIDQGRVISGPLEQQEFGESPWQTDWLYFLPEFNMKKWLNFSAMAGPGPVL
                     AKEQSAEVPMGLLIPCLSSGDSGFVEAMSECVDAIYHDEGARKIFSKTARKIFKEALQ
                     KNITIKAYKVS"
     gene            2871..>3444
                     /locus_tag="PLE23_11345"
     CDS             2871..>3444
                     /locus_tag="PLE23_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMX83903.1"
                     /translation="MIHKCIIIGSGPAGYTAAIYAARANLSPMMFTGPEPGGQLMITT
                     EVENFPGYPEGVQGPQMMEDFRQQAERFNTVIKYEQISKVDFTGPVHKIWNEAGEEFQ
                     AHSIIISTGASAKWLGIDSEKRLLNKGVSACAVCDGFFFRNLDVAVVGAGDTAAEEAS
                     YLAKLSPNVHMIVRRDEMRASKIMQDRVKNT"
BASE COUNT         1005 a          656 c          757 g         1026 t
ORIGIN      
        1 aatattagct gattattgct tgtctaatac agctctgacg ctaccgtatt ttaagagtag
       61 ttcttttgct tgctcgaagg aaattgattt tttttccata atcatcttga cgccacggtt
      121 tattaattta tggttgctca actgcatatc taccatttta ttatctgcca catgacctag
      181 ttggatcatt gcaacggttg atatcatgtt gagtaccatt ttctgagctg tacccgactt
      241 catccgggtg ctcccggtca ggaattcagg accaacaacc acctctatcg gataatcaga
      301 aacctgagca acaggtgatg aagcattaca ggtaatacag ccggtagcaa tgccttgttg
      361 gcggcatttt tcaagcccgc caatgacata tggtgtggtg ccggatgcgg caatacctat
      421 aacgaagtct tttgtgctga tgttgtagcc tgacagcatt ttccaggcaa ggtctgtgtc
      481 atcctcggca aattctactg cttttcttat ggcgctgtca cctccggcaa tgataccgat
      541 gacaagtccg tggtcaactc caaatgtagg cggacattcg gatgcatcaa gaataccgag
      601 tcggcctgaa gtacctgcac caatgtaaaa caatctgcca ccttctttca tgaagccgac
      661 caatgcggta ataaggctgt gtatttgagg gatggacttc ctgacagcag cagcaacttt
      721 agaatcttca tcatttatgc ccaagagcaa ttcatttaca ggcatttttt ccaggtgcct
      781 gtattctgat gaagattctg ttgttagctg catattagta ttaagctgct gagcttttta
      841 cataagcatc agtcatttct tttgagatgg ttgacttcaa aaatttaagg taagttttac
      901 cttctatctc tagttgtaca gattgttcgt caatctttgt gattcggcct atgatgcctg
      961 acatggtgac cacttcatct cctttttgaa ggttggcaac gaacatgttt tgttcttttt
     1021 gtttctttga ctgaggtcga atgaaaaaga aataaaatac gacgaataat agcagaaagg
     1081 gtagtaattg gcctaatgcc tccatggtag tagttttttg ttatttaaat ggttttattt
     1141 gacaattcct gtgatgtaaa ttttattttc ggccggaagt gtattagcat atataaacac
     1201 aggcttattc tgtgcatctt ttttaccttc cgaattaaag accaccttga tagatgccgt
     1261 atcgcccgga gcaattggag ttttgggcca ttccggtaca gtacacccac atgttgaatg
     1321 accatcactt ataatcaaag gagtttttcc gacatttttc agtttaaaaa aatgtgtaat
     1381 gacttgaccc tgatggatgg ttccaaaatc aaaagaagtt tcctcaaact ggatttttgc
     1441 cattttagag gtatctttaa ttccttcagc tgtgaccggg ctttttataa gctctgcgac
     1501 agagcccggc ttggaaggtc ttatttcaac aacttcatcg gcatcactat gcttgcaggc
     1561 atttaagaaa aatgcagcag tcaaaaatat aatcgtaatg agcatcttgt tcattcggca
     1621 aatgtagtaa ttttagtgta aatttaataa caaatgcttg aaaagacaga tgaccaaatg
     1681 gatataaatg acattcagat gattttcatt atcggattta tgggatcggg caaaacaacc
     1741 attggacgtt tgctagccaa caggctcggc tggaatttta ttgatctcga cactttgata
     1801 cagataagag aaggacggag catacccgat atttttgaat cgcactcgga agcgtttttc
     1861 agaaaggcag aatacaacgc ccttcgtcaa ttgggaggct tgaaaaatat agtagtgtca
     1921 accgggggag gtacaccttg ttactttgat aatattcatt tcatgaaatc aacgggcaag
     1981 gtcatttggt tggataatga tttcggtaca ttgtacaaac gcattatagg tacacaatcc
     2041 ggtacaagac ccttagtgtc cggaaattct aaagaaaaac tgaaagaatt gtttgtcacg
     2101 agaggggaat attataagtt ggcagatgta aagatatctt gtgagccaga cgatttgccc
     2161 gaagcgatct tagcaaagat agttaagttt ttcttgtggc acacaccaat ttatactgtc
     2221 acaaaaatca taatacaggt atttttgagt gaattacttt gaaacatctt tgcagtacca
     2281 taaaaataac gagtgctaaa gcctgattca tgaagaaatt tgggtttatt gttgatttgt
     2341 ctgatactgc acaggaaaag tacgagcaga tagtggatgc ttttgatagg tttagtgatg
     2401 aacttagtcc gaagcattta aaaaacaccc aattgcgtat tatccttcct ccgggatcac
     2461 cttacattga ccaaggtagg gtcatttccg gtccgctaga gcagcaggag ttcggtgagt
     2521 cgccctggca gacagactgg ctttattttc tgcctgagtt caacatgaaa aaatggctta
     2581 atttttccgc aatggcaggc ccgggacctg tactcgccaa agagcagagt gccgaagtgc
     2641 caatgggtct attaatcccg tgcctgtcaa gtggagattc aggatttgtg gaagccatgt
     2701 cagaatgtgt agatgccatt taccacgatg aaggtgccag gaagattttt tcaaaaacgg
     2761 ctaggaaaat ctttaaagaa gctctccaaa aaaatattac tattaaggca tacaaggttt
     2821 catagtattt tttttattag tatctttgcg gctcattaaa aaaatatttt atgattcata
     2881 agtgtataat tataggttca gggccggcag gctacactgc tgctatatat gcggccagag
     2941 ccaatctgag cccgatgatg tttacaggtc cggaacccgg aggtcagttg atgatcacga
     3001 cagaggtgga aaattttccg ggatatccag aaggagtaca ggggccgcag atgatggaag
     3061 atttcaggca gcaagcagaa cggttcaata cggtcatcaa atacgaacaa atttccaagg
     3121 ttgactttac aggtccggta cataagatat ggaacgaagc cggtgaagag tttcaggccc
     3181 attctatcat catttcaaca ggagcaagcg ccaaatggct cggaatcgat tcagaaaaaa
     3241 gattgttaaa caaaggagta tcggcttgtg cagtgtgcga tggctttttc ttcagaaatc
     3301 ttgatgtagc ggttgtagga gcaggagata cagctgcaga agaggcatca tatctggcaa
     3361 agctttctcc taatgtgcac atgatagtgc gaagagacga aatgcgtgct tccaaaatca
     3421 tgcaggaccg agttaaaaac acaa
//
LOCUS       DAOQQY010000291         3439 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13657_length_3439_cov_6.153073, whole genome shotgun sequence.
ACCESSION   DAOQQY010000291 DAOQQY010000000
VERSION     DAOQQY010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13657_length_3439_cov_6.153073"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2608)
                     /locus_tag="PLE23_11350"
     CDS             complement(<1..2608)
                     /locus_tag="PLE23_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83904.1"
                     /translation="MNERLKVLRLLFYSIVSLMFLPDIYATHNRAGEIIVEQTGDCND
                     LTVKATIVTYTKASSAAADRDSLTICWGDGTCQVIARTNGPKGGLNGVPQGQFIGNDT
                     KKNLYVAVHTYPGRATYVISMTDPNRNGGILNVNPPASDVVPFHIQTTYTFLNSQFQG
                     CNSTPVLLKSPIDFACVGQPFKHNPSAFDIDKDSLSYHLIVPLQGVGSPVPNYFFPHQ
                     IKPGVNNNITLNPKTGDLIWNSPQLEGEYNIAMIIVEYRQGVAIDTMVRDMQIFVQTC
                     DNKPPDIMAPSELCVLAGEKIAFDVTATDPDVPFIQKVKLSAVGGPFVVSKSPATFTV
                     AAGHVDQPNIGKFEWQTACEHISEQVYSLIFTAEDNYLSPTSGFTNLKTVLIKVVGPP
                     PLNLNADSQNDVVKLTWDDPYACDEIDDNYFFGFSVWRREGSNPFVPDSCETGLAGKG
                     YTRIAFKQKAADAGKYLYDDKNVEPGRNYCYRIVAEFAKLSAGGNPYNIVESIPSKEA
                     CIQVNRDVPLMTNVSISTTSNTAGVIEVRWSKPSATDLDTLKYPGPYRYQLLRAEGLS
                     GGSFVEIPEANFIKNQFWQANDTIFTDNNPLNTLQQAYSYKVIFYADATGNPVGTTQP
                     ASSVFHSVASSDKLNVLSWQANVPWQNYRTTIYRLNDITGLFDSIASVTDAEYTDTKL
                     VNGKEYCYKVKTSGTYGIGGIASPLYNFSQEVCGTPLDTVPPCAPQLAVSNPCAENNP
                     ELPEDLFNNLLTWNTSPQGCQNQNDAVEFKIYFKGPTDDTFELIETLPNNGVYQYSHK
                     PKTGIAGCYYVTAVDSVGNESHAGYTVCVDNCPVYILPNTFTPNNDGQNDIYHPRISR
                     FVERIDMKIYDRW"
     gene            2872..3198
                     /locus_tag="PLE23_11355"
     CDS             2872..3198
                     /locus_tag="PLE23_11355"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HMX83905.1"
                     /translation="MSWIILIAAGIFETAFAFCLGKAKLSHGTEMYLWYLGFVISLLA
                     SMLLLIKAIQSIPIGTAYAVWTGIGAVGTVLTGILYFQEPVTFWRMFFITSLIVSIIG
                     LKTFAE"
BASE COUNT          869 a          801 c          767 g         1002 t
ORIGIN      
        1 cccatcggtc gtatatcttc atatcgattc tttctacaaa gcggctgatt cttgggtggt
       61 agatgtcgtt ctggccgtcg ttgtttggtg taaatgtatt gggtagtata tacacagggc
      121 agttatcaac acaaactgtg tagcctgcat ggctttcatt cccgacagaa tctacagctg
      181 tcacataata gcatcctgca ataccagtct ttggcttatg gctgtattga tacacaccgt
      241 tgttaggtaa agtttctatc aattcaaatg tatcatccgt cgggcctttg aaatagattt
      301 tgaactcgac tgcatcgttc tgattctggc aaccttgcgg tgatgtattc caggtaagca
      361 ggttattgaa caaatcttca ggcagctccg gattattttc tgcacaagga ttacttactg
      421 caagctgagg agcacaagga ggtactgtat cgagaggcgt tccacatact tcctgagaga
      481 agttgtacag cggactcgca ataccaccta tgccgtaagt accacttgtc tttaccttgt
      541 agcaatattc cttaccgttc acgagcttgg tatcggtata ttcagcatct gtcactgagg
      601 ctatactgtc gaataggcct gtaatgtcgt tcagcctata gattgtggta cggtagtttt
      661 gccatggcac atttgcctgc cagctcagta cattcagttt atcacttgaa gcaacgctgt
      721 ggaataccga agaagccggc tgggttgttc cgacaggatt acccgtagca tcagcataga
      781 aaatcacttt gtaagaatat gcctgctgaa gtgtattgag cggattattg tctgtaaaaa
      841 tggtatcatt cgcctgccag aactggtttt ttataaaatt ggcttcgggt atttcaacaa
      901 aactgccacc tgaaagacct tctgcccgta gcagctgata ccggtacgga cccggatatt
      961 tcaaggtatc gaggtcggtg gcacttggtt tagaccacct cacttctatg acaccggcag
     1021 tgttacttgt ggtacttatg cttacattag tcatcagggg cacatcgcgg tttacctgta
     1081 tgcatgcttc cttagaggga atgctttcga cgatattgta aggattacca ccggccgata
     1141 gtttggcgaa ttctgctacg atgcggtagc agtaatttct gcccggctct acgtttttgt
     1201 catcgtataa atattttccg gcatctgcag ctttctgctt aaaggctata cgtgtataac
     1261 ctttacctgc gaggcctgtt tcgcaactat cgggcacgaa cggattgctg ccttctcgcc
     1321 tccagaccga gaacccgaag aaataattgt cgtcgatttc atcacaggca tacggatcgt
     1381 cccaggtgag ttttactacg tcattctggc tgtctgcatt caggtttaag ggtggcggcc
     1441 ctaccacctt aataagtacg gtcttgaggt tggtaaagcc tgatgtcgga gaaaggtaat
     1501 tatcttcggc agtgaatatc aggctataca cttgctcaga gatatgttca cacgcagtct
     1561 gccattcaaa tttaccaatg ttgggctgat cgacgtggcc ggcagcaacc gtaaaggtag
     1621 caggactttt gctcactacg aacggacctc cgacggcact aagttttact ttctggatga
     1681 aaggcacatc tgggtcggtg gcagtcacgt caaaagctat tttttcgcct gccaacacac
     1741 ataattctga aggtgccatt atatcgggcg gcttgttgtc gcatgtctgt acgaatatct
     1801 gcatgtcgcg taccatagta tctatggcca ccccttgccg gtattcgacg atgatcatcg
     1861 ctatgttgta ctccccttca agctgtggcg aattccagat gaggtcgccg gtcttagggt
     1921 tgagtgttat attgttattg actccgggtt ttatctgatg cggaaaaaaa tagttcggca
     1981 ccgggctacc tacaccttgc aacggaacga tcaagtgata gctgaggctg tctttgtcga
     2041 tgtcaaaagc tgaaggattg tgcttgaatg gctgccctac gcatgcaaaa tcaatgggcg
     2101 actttaacaa taccggtgta ctgttgcagc cctgaaactg cgaattgagg aacgtatagg
     2161 tcgtctgtat gtgaaatggc accacatcag atgccggcgg atttacattc agtatgccac
     2221 catttctgtt agggtcggtc atggatatca cgtaagtggc acgccccgga taagtatgaa
     2281 cggccacgta gagattcttc ttggtgtcat tgccaataaa ctggccttgt ggcacaccat
     2341 ttagtccgcc tttgggcccg ttggtacgtg ctatcacctg acaggtgcca tctccccaac
     2401 agatggtaag gctgtcgcgg tcggctgctg cgctgctcgc cttagtataa gtgacgatgg
     2461 tagcctttac cgtcaggtca ttgcagtcgc ccgtctgctc tacgatgatc tctccggcac
     2521 ggttatgcgt agcatatata tccggcaaaa acatcaggct cacgatgcta taaaacaata
     2581 acctcagaac tttcagtctt tcattcataa gctaaagata atgcctgatt tgaaatattg
     2641 ccactatttt ttttaagttt aacaaaatgt ccaaattact gttcagctca cagactttgt
     2701 attgaaccca aatagcacat taggctcttt actgccaata atgattacta caaatccatt
     2761 gcaaaagctc aaaaaccata gtttgctttt ggctttactt cgtcccggta agctttcagt
     2821 acattttatc catacaattt tccgatattc gttccaaaat aaagataaac catgtcctgg
     2881 ataatattaa tagctgccgg aatattcgaa acagcattcg ctttttgcct cggcaaagcc
     2941 aaactaagcc atggtactga gatgtacctt tggtatttgg gttttgtcat ttcattgctg
     3001 gcaagtatgc tgctactcat caaagccata caaagcatac ccataggtac agcctatgca
     3061 gtatggaccg gcataggcgc agtaggcaca gtattgaccg gtatccttta ctttcaagaa
     3121 ccagtcacat tctggcgtat gtttttcatt accagcttaa ttgtatccat cattgggctc
     3181 aaaactttcg cagaatagtg caaaaaatct actttcacca ggcaggctca ccgatctttc
     3241 cacaatacct acaacacaac aaaccggcga tgtagctttc acgccaacct atctgatgac
     3301 agtgtagtgc ctagaccacc agagtatagc ataaagttcc ttacataaca taacagaccc
     3361 cttaacaatg aaggatactg tgagtagggc gtgcccctgt cgggtcaggc catccgcttt
     3421 acttcgtgtt cgcttctgt
//
LOCUS       DAOQQY010000292         3415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13798_length_3415_cov_4.820536, whole genome shotgun sequence.
ACCESSION   DAOQQY010000292 DAOQQY010000000
VERSION     DAOQQY010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13798_length_3415_cov_4.820536"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="PLE23_11360"
     CDS             complement(<1..531)
                     /locus_tag="PLE23_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83906.1"
                     /translation="MRRIYFTLLILAVSSSLIMSCSAGGGLLHGNFTGAANQPLKLVH
                     YGQNNMPTDVASTTIGANGDWKFDVKELKPGFYRLEVGKEDQNVIPRLYTVFNGKEKS
                     IKITGNVADFPKFGVNIEGSDASTEFLSVIKPLAEGSNPDREVIIKQVVGAKDVLVSM
                     ALSNILFRGMPDFLDSY"
     gene            complement(541..2781)
                     /gene="purL"
                     /locus_tag="PLE23_11365"
     CDS             complement(541..2781)
                     /gene="purL"
                     /locus_tag="PLE23_11365"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HMX83907.1"
                     /translation="MQVNDVLPDLVTAKNLGLTEDEFEKIKQILGRHPNFTELSIYAV
                     MWSEHCSYKNSIKYLKTLPRDGKKLLVAAGEENAGLIDIGDRLACAFKIESHNHPSAL
                     EPFQGAATGVGGIHRDIFTMGARPIASLNSLRFGNIETPHMQRLIKGVVSGIGHYGNC
                     FGVPTVGGEIFFNDCYNQNILVNAMSVGIVKHGETVSATAAKPGNLVLIVGSATGKDG
                     IHGATFASADLTEDSAEDLPAVQVGDPFQEKLLLEASLEAIAANAVDGMQDMGAAGIT
                     CSTSEMSAKAGLGMKIDLSKVPTRQKNMHAWEILLSESQERMLVVCEPGKEKILFDIY
                     DKWDLECEIIGEVTEGPMLEFYEGNTLVAEVPAESLVLGGGAPVYDREYTSPEYLEKI
                     KAFKTSSIPTPDDIVQTAKKVMSSPNLVSKRWIYEQYDSMVRTNTVTTNAPSDAAVSY
                     VKGTSKAIAMTTDCNPAYVFADPFKGTMIAVSEAARNIVCSGGEPVGVTNCLNFGNPY
                     NPEVYWQFVHAIKGMGEACRRFETPVTGGNVSFYNQSQIGNQTIPVYPTPTIGMVGIL
                     EDASNHTTLDFKNTKDIIYLLGQTQNDLGSSEYIRSIYNVEYSNCPYFNIDDEIKLHD
                     VIKKLIKQKIVSSVHDVSDGGMLATLLESAFVNDKGFKIDTEDNIRIDVALFGESQSR
                     AIISMSETNEARIQELLLNNNIPFTRLGVVTDGEITVNNIDFGQITDWKKTFETKIEN
                     ILEHKS"
     gene            complement(2850..>3415)
                     /locus_tag="PLE23_11370"
     CDS             complement(2850..>3415)
                     /locus_tag="PLE23_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HMX83908.1"
                     /translation="RILLVLFIVGMTMLFLSLTAAYIYNRIVAPSMPPIKVPVLFVFN
                     TLVLLGSSLSLMRAKQSYRKDDTEAYKYNLLITIGLTVIFLLLQILAWKSLFEANISI
                     THNNLASYLYMLSALHFLHVIGGLPFMLNFYFNALKNMKEPVSVLIYFSDPFKKLKLN
                     ILSLYWHFLDILWIYLVLFLWVNQLIK"
BASE COUNT          949 a          700 c          685 g         1081 t
ORIGIN      
        1 gtaagagtcg agaaaatcag gcatacctct gaacagaata ttagataaag ccatagaaac
       61 aagtacgtct ttagcaccca caacttgttt gatgataacc tctctgtccg ggttagatcc
      121 ttctgcaagt ggttttatta cacttagaaa ttctgttgaa gcatcagagc cttcgatgtt
      181 tacaccaaac ttaggaaagt cagctacgtt acccgtgatt ttgatgcttt tttccttgcc
      241 attaaatact gtatatagac ggggaatgac attttggtct tctttgccaa cttcaagtct
      301 gtaaaaaccc ggtttgagtt ctttaacgtc aaatttccag tcgccattgg caccgatggt
      361 agtgcttgct acatcagtag gcatattgtt ctggccataa tgtactagtt tgagaggctg
      421 gttggcagct cctgtaaaat tgccatgaag taatccgcca ccggcagaac aactcataat
      481 aaggcttgaa gaaactgcga gtatcagtaa agtaaaataa attcttctca tgttttgtgt
      541 ttaagattta tgttcaagaa tattttcaat ttttgtttca aatgtttttt tccaatctgt
      601 tatctgacca aagtcaatgt tgtttactgt tatctcgcca tccgtaacaa ccccaagtct
      661 tgtaaatgga atgttgttgt tgagtaataa ttcctgaatt cttgcttcat ttgtttcact
      721 catggaaata atggctctac tctgagattc tccaaataac gctacatcaa tcctgatatt
      781 atcttccgta tcaattttga atcctttatc gttgacaaag gctgattcta gtaatgttgc
      841 cagcattcct ccatcgctta catcatgtac ggaagaaaca attttttgtt tgataagttt
      901 tttgattaca tcatggagtt ttatttcgtc atctatatta aaataagggc aattactata
      961 ctctacattg taaatagacc gtatgtattc cgaactgcct aagtcgtttt gagtctgacc
     1021 cagcagataa ataatatcct ttgtattttt gaagtcaagt gtcgtgtggt tgcttgcatc
     1081 ctctagtatg cctaccatgc caatggtagg tgtaggatat acaggtatag tctgattccc
     1141 aatctgcgac tgattgtaaa aactcacatt gccacctgta accggcgttt cgaatcgcct
     1201 gcaagcttcg cccattcctt tgatggcatg cacgaactgc cagtacactt ccgggttata
     1261 aggattgcca aaattgagac aatttgtaac tcctacgggt tcgccacctg agcacacgat
     1321 gttgcgtgct gcttcagaaa cggcaatcat tgtacctttg aacggatctg caaatacata
     1381 agccgggttg cagtcggttg tcatggcaat ggctttagat gttcctttga cataactcac
     1441 agcagcatca gatggagcat tggtagtgac cgtattggta cgtaccatcg aatcgtattg
     1501 ttcgtatatc catcttttcg aaaccagatt tggagagctc attactttct tagctgtttg
     1561 gacgatgtca tcaggtgtcg gtattgagga ggttttgaat gctttaattt tttcaaggta
     1621 ttcgggtgaa gtgtattcgc ggtcgtacac cggggcacca ccaccgagta cgagtgattc
     1681 ggccggcact tctgccacaa gggtgtttcc ttcatagaat tccagcatag gtccttcagt
     1741 cacttcgcca attatttcac attccaaatc ccacttgtca taaatgtcaa atagtatttt
     1801 ttccttgccc ggctcgcata ccacgagcat tcgttcttgg ctttcagata gtagtatttc
     1861 ccaggcatgc atgttttttt gtcgtgtcgg tacttttgag aggtctattt tcatgcctaa
     1921 acctgccttg gcactcattt ctgaagtact gcatgtaatg ccggcggcgc ccatgtcctg
     1981 cataccatcg acggcatttg cggcaatagc ttcaagtgat gcttcgagca gtaatttttc
     2041 ctgaaaaggg tcacctactt gaacagcagg tagatcttcg gcagaatctt cagttaaatc
     2101 tgctgatgca aaggtagcac catgtatgcc atctttgcct gtggcagaac cgactatcag
     2161 caccagattg cccggtttgg cagctgttgc agagacagtt tctccgtgtt tgacgatacc
     2221 tactgacatt gcatttacca gaatgttctg attgtagcaa tcattaaaaa atatttctcc
     2281 acctactgtt ggtactccaa aacaattgcc ataatgcccg ataccgctta caacaccttt
     2341 gataaggcgt tgcatgtgag gggtttcaat attgccaaaa cgcagagaat ttaaggaagc
     2401 aatcggtctg gcacccatag taaaaatatc tctgtgtatg cctccaacac ctgtggcagc
     2461 accctgaaac ggctcaaggg cacttgggtg gttgtgcgat tcgatcttga atgcacacgc
     2521 aagcctgtca cctatatcta ttaggccggc attttcttca cctgcggcta ccaatagttt
     2581 tttaccatca cgaggaagtg ttttgagata tttgattgaa ttcttgtacg aacaatgttc
     2641 tgaccacatt acagcataaa tgctgagttc tgtaaaattg gggtggcgac caaggatttg
     2701 tttaattttt tcaaattcat cttcagttag accaaggttt ttggcggtca ccagatctgg
     2761 gagtacatca ttcacttgca taagttattt ttgaaatttg ccacaaaagt acatgctttt
     2821 gacggactaa tatttatatc ggtcaggttt tatttaatta actggttgac ccaaaggaat
     2881 aatacaagat aaatccatag aatatccaaa aaatgccagt ataaactaag tatgttgagc
     2941 ttcagtttct tgaacggatc tgaaaaatat atgagtacac tcaccggttc tttcatgttt
     3001 ttcaatgcat tgaaataaaa attgagcata aatggaagtc cgccgatgac atgcagaaaa
     3061 tgcaatgcag ataacatata caagtatgag gcaagattat tgtgtgttat actgatattc
     3121 gcttcaaata atgacttcca ggctagtatt tgaagtaaca aaaatataac agtaagccca
     3181 atcgtgatga gcagattata cttgtatgct tctgtgtcat cttttctata gctttgtttt
     3241 gctctcatga gcgacagact acttccaagc aatacgagcg tattgaaaac aaacaatacc
     3301 ggtaccttta ttggaggcat cgaaggtgct acaattcggt tgtagatata ggctgcagta
     3361 agggagagaa aaagcattgt cataccgaca ataaataaga caagcagaat cctgt
//
LOCUS       DAOQQY010000293         3395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_13924_length_3395_cov_5.604491, whole genome shotgun sequence.
ACCESSION   DAOQQY010000293 DAOQQY010000000
VERSION     DAOQQY010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13924_length_3395_cov_5.604491"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..336)
                     /locus_tag="PLE23_11375"
     CDS             complement(19..336)
                     /locus_tag="PLE23_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005375828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HMX83909.1"
                     /translation="MLLTTTPNIEGRPIQKYLGIVTSEAIIGANFFKDIKATLTDIFG
                     GRSTSYEKVLMEAKDHALEELKQRAAAMGANAVVGIDLDYETVGGAGGMLMVTASGTA
                     VVI"
     gene            770..2002
                     /locus_tag="PLE23_11380"
     CDS             770..2002
                     /locus_tag="PLE23_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMX83910.1"
                     /translation="MMRFLIPFIILLLLDIYVFQVVASAARDFGRLQKMLVYIVFWLT
                     PVIAFLIMYSAGRGYFALWPKYISSMAMAFMFIVYFSKVFAAIPLIVDDVRRFFVLVA
                     GKIFPSTNFDAGRSDFISKFALLLGGLPFGLLSYGVLRNPYRYKLRRQDIYLRNLPDE
                     LDGFKIVQISDIHSGSFFMKEPVKSSVAMINTCKPDIVFFTGDLVNNKADEIDDFIDV
                     FDKIEAKYGVYSILGNHDYGDYHHWDSAEQKHENFRKMIDAHKRLGWKLLMNENEKIS
                     LGNATLGIIGVENYSMAMHFPKYGDLAKAYKGVEDADVKILLSHDPTHWDGQVIKDYK
                     DIALTLSGHTHGFQFGVEIPGWIKWSPSQFVYKRWAGLYTQHDQYLYVNRGLGFLAYP
                     GRVGILPEVTELVLKKML"
     gene            2056..2409
                     /locus_tag="PLE23_11385"
     CDS             2056..2409
                     /locus_tag="PLE23_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83911.1"
                     /translation="MNKIILLISALAMVACKTDKPTIGPQELHGSWDIIQATRDGEIF
                     DALAGGYFEFANTNIFQTNIPSLPNGVAFVVESEKIRFEGDTLKYKITNYSDTLLVLE
                     SNIRGSAFQFTCKRR"
     gene            2477..>3395
                     /locus_tag="PLE23_11390"
     CDS             2477..>3395
                     /locus_tag="PLE23_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83912.1"
                     /translation="MLWFRFTLIFGLLTGFFTSASAVEVNLAIYKFNGPALPYVEIST
                     NFIGNTLKLQKIDTIAHQATLEVTTVFMLGEKIVKADKYLLNGPLTVRPVDFLDVKRY
                     SLEPGQYRMLTHFVDMNDTTNQLSTAVEFSLFFEKAKVQFSDIQLLSDARKAEVEGPM
                     VKSGLYMVPVAGNYFPKQFATLFAYLEVYHAESTISKKFGLCSYIERADQAGGKIDGY
                     THCKTYHTSNVVPIILQKNIALLPSGNYNFVIEFRDTLGNILFDRKAFFQRSNPSADI
                     ASVKVDEANYENSFIKSLDESTVKYALRAL"
BASE COUNT          980 a          635 c          692 g         1088 t
ORIGIN      
        1 tgattcagca aaaaccgatt atattactac agctgttccg cttgccgtca ccattaacat
       61 gccaccagca ccacctaccg tttcgtaatc gaggtcgatg cctactactg cattggcacc
      121 catagcagcc gccctttgct tcaattcttc caatgcatgg tctttggctt ccatcagtac
      181 tttttcgtaa ctggtcgaac ggccaccaaa aatatcggtc agtgtggctt ttatatcttt
      241 gaagaaattg gcgcctataa tggcttcgct tgtgacgatg ccaaggtatt tttgtatcgg
      301 gcgtccttcg atgttaggtg tggtagtcag caacataatt gtatttgttt atatttttgt
      361 tttattcaaa tctcaacaaa tatattgatt ttgaatagaa atatctattc gtgttaaaaa
      421 agtttagtgt tctctccaat ttcagtagta atatttgatg tatgtaaaat attcattcat
      481 ccgaaacttt ttaagcacga cgtaattgtg cgaaatactt gtaagttgac attcaggtgt
      541 cttacagatt tgacccgaat tgcatgatgt gatttcgtga gtatgctaag tttttcattg
      601 gttagtatta agttgacatt cattcctctt tccggaaatg aggcaaattt ttcggaattt
      661 catgtgtaat ctgctgatga cggaagcaag tcggtacgga taatcgcaag taaaaatcat
      721 tggaatgcca caacaaaata cccaaaactg cattatgtac cttatttgaa tgatgcgttt
      781 cttaattcca tttattattt tactactgct agatatatat gtttttcagg tagtagcaag
      841 tgcagcgcgt gattttggga ggctacaaaa aatgttggtt tatatcgttt tttggctcac
      901 tccggtcatt gcgtttttga ttatgtattc tgccggccgc ggatattttg ctttgtggcc
      961 caagtatatt agctcaatgg caatggcatt tatgttcatt gtttattttt ccaaagtatt
     1021 tgcagctatt ccgcttattg tagatgatgt aaggagattt tttgttttgg tggccggtaa
     1081 aatattccct tcgaccaact tcgacgccgg ccgttccgat tttatttcga agtttgcatt
     1141 gttgcttggt gggctgccat ttggtttatt gtcgtatggt gttttgagaa atccatatcg
     1201 ctacaaactc agacgccaag acatttacct tcgcaatttg cctgatgaac ttgatggttt
     1261 taagattgta cagatttctg acatacattc cggaagcttt tttatgaaag agcctgtaaa
     1321 aagctctgtt gccatgatta atacatgtaa accggatatc gtattcttca cgggcgatct
     1381 ggtcaacaat aaggctgatg aaatagatga ttttattgat gtttttgata aaattgaagc
     1441 gaaatacgga gtttactcca tacttggcaa tcatgattat ggtgactacc accattggga
     1501 ttctgctgag caaaaacatg aaaattttcg gaagatgata gacgcccaca agcgactagg
     1561 ctggaaactg ctcatgaacg aaaatgaaaa aataagcttg ggtaatgcga cacttggtat
     1621 aataggtgtc gaaaactatt caatggcaat gcactttccg aaatacggcg atctggcaaa
     1681 agcctacaaa ggtgtcgaag atgcagatgt caaaatactg ctaagccatg atcctacaca
     1741 ttgggacgga caagttatca aagattataa agatatagcc cttacattat caggtcatac
     1801 tcacggattc cagtttggtg tagagatacc cggatggata aaatggagcc caagtcagtt
     1861 tgtatataag cgttgggcag gtctttacac tcagcatgac cagtatttgt acgtaaaccg
     1921 cggtctggga tttttggcgt atcccggcag ggtaggtata ttgccggagg tcacagaact
     1981 ggtgttgaaa aaaatgttat aaaaatattg aaacatgaaa ctatttttgc agaatatttg
     2041 tttcacacaa taattatgaa caaaattatc ctgttgattt ctgcacttgc aatggttgcc
     2101 tgtaaaacgg acaaaccaac tataggacca caggaattgc atggcagttg ggacatcatt
     2161 caggctactc gtgatggtga aatatttgat gccttggcag gtggatattt cgaatttgcg
     2221 aatactaata tttttcagac taatattcct tcattaccca atggtgtggc attcgttgtc
     2281 gaatccgaga aaatacgctt tgaaggcgat accttgaaat acaaaattac caattattcg
     2341 gatacattgc tcgttcttga gtcgaatatt cgtggttcag cttttcagtt tacttgtaag
     2401 agaagatagc tccaacaaaa aaacttgcat cattcgctta ttttggtgaa tttctcttta
     2461 tttttacaaa aaaaacatgc tctggtttag gtttacattg atttttggac tattgaccgg
     2521 tttttttaca tctgcgagtg cggttgaggt caatcttgct atatacaaat tcaatggccc
     2581 tgctttgcct tatgtagaaa tttcgaccaa ctttatcggc aatacactca aactacaaaa
     2641 aattgataca atagctcacc aggccacttt ggaagtaacc accgtgttta tgcttggcga
     2701 aaaaatcgta aaagctgata agtacctgct caatggccct ttaaccgtca ggcctgtaga
     2761 tttcttggat gtaaaacgtt attcgctcga acccggacag tacaggatgc ttacacattt
     2821 tgtggatatg aatgacacaa ccaaccaact gagtacagct gtcgagtttt cattattttt
     2881 tgaaaaagcc aaagtgcagt tttcagatat tcagctgctg tcagatgccc gaaaagctga
     2941 agttgaaggc ccaatggtca aaagtggtct ttatatggtg ccggtagccg gcaattattt
     3001 cccaaaacag ttcgctactt tgtttgccta ccttgaagtg tatcacgcag aatcaacgat
     3061 tagtaagaaa tttggcttgt gtagttatat cgaaagagct gaccaggcag gtgggaaaat
     3121 tgatggatac acccattgta aaacctacca cacctcgaac gtggtaccca ttatattaca
     3181 gaaaaatatt gcactgctac cttcaggtaa ttacaacttc gtgattgaat tccgtgatac
     3241 acttggcaat attttattcg accgcaaggc gtttttccaa aggagtaatc catccgccga
     3301 catcgcttcg gtcaaggtag atgaagccaa ttatgaaaat tcattcatca aaagcctgga
     3361 tgaatcgaca gtaaaatatg ctttacgtgc tttgg
//
LOCUS       DAOQQY010000294         3374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14054_length_3374_cov_6.418500, whole genome shotgun sequence.
ACCESSION   DAOQQY010000294 DAOQQY010000000
VERSION     DAOQQY010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14054_length_3374_cov_6.418500"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLE23_11395"
     CDS             complement(<1..513)
                     /locus_tag="PLE23_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMX83913.1"
                     /translation="MQNLDLVGKRILVRVDFNVPLDNHLNVTDNKRIREALPTIEYLL
                     SLDGVSVILMSHLGRPLKKLLPDGSIDVQKFSLRNIIPELSKLLGREVLFAENCIGEK
                     TEAIAKSLIAGQVLLLENTRFHKEEEKGDEEFSRQLASLGQAYINDAFGTAHRAHAST
                     CIIARYFAKNV"
     gene            complement(645..2321)
                     /locus_tag="PLE23_11400"
     CDS             complement(645..2321)
                     /locus_tag="PLE23_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMX83914.1"
                     /translation="MISWIDWSVLLGSLVFITLFGIWKTKKNSSTRAYLMGDEELNWW
                     TIGFSIMATQASAITFLSTPGQAYEDGMRFIQFYFGLPLAMIVLSVFILPQYYKLKVF
                     TAYEFLEQRFDVRVRTLTAVLFLLLRGLSASITLIAPAIILSALFGWSMAFTIFMLGS
                     FVIVYTLIGGSNAVSKTQVQQMAIILLGLFMAIILIYSQLPAGIGIKETFSIAGSAGK
                     MNIVDFKFDPKERYNIWSGLLASFFLFLSYFGTDQSQVQRYLTGKTLTESRLGLMFNG
                     LVKIPMQFTVLLTGLMVYIFFLFQQPPLHFNEVNRQNVMKTEVANEYKNLEAKFDVVF
                     NDRKTFITENIAKDNLSDETFSQKLKDYNKKIRELRLEAREKISQANPSAKVKDEDYI
                     FMYYVLNYFPVGVIGLLLAMIFSAAMSSTASEINALATTFIVDIYKRNWVKDATDEHY
                     LKMSKTVTLGWGILLMIFALWAAPLENLIQAVNILGSLFYGTILGVVVSAIFLKKVSA
                     NGVIVGAIVAETVVILGYFFSDIGYLWFNAIGCILVMFIGSAYSAIFNKK"
     gene            complement(2318..2449)
                     /locus_tag="PLE23_11405"
     CDS             complement(2318..2449)
                     /locus_tag="PLE23_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83915.1"
                     /translation="MKNNNSEKIPVFGKWPGWYLFVGIFNLLLLVVFILLTSIFNHK"
     gene            complement(2454..>3374)
                     /locus_tag="PLE23_11410"
     CDS             complement(2454..>3374)
                     /locus_tag="PLE23_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LmbE family protein"
                     /protein_id="HMX83916.1"
                     /translation="AAKDNMEGTVSVTTTNGWICSNNNQKFIIKKKGDEQVFSFTISP
                     PSAKSETDINFIATVKGQEFRNSMVEVAYDHIPTQRVLKPSYSRLVHDDIKCSPVKIA
                     YLTGAGDKIPEALKQLGIDVHFIKAEEIELEKISNYDVILTGVRAYNTLQMLANKHEI
                     MMKFVENGGTLICQYNTAFGLVTKDIGPYPFTISRGRVTDENAPMQMLDKDHRIFNMP
                     NKLTNADFEAWVQERGLYFITTDDTRYQKLLKTHDPGEQDLDGSLVTCKYGNGNFVYC
                     SLSLFRQLPVGVPGAYKLLANMLSLGVQQN"
BASE COUNT          968 a          684 c          666 g         1056 t
ORIGIN      
        1 tacatttttt gcaaaatacc tcgctatgat acaggtagat gcatgtgcac ggtgtgcagt
       61 tccgaatgcg tcattgatat atgcctgtcc gagtgaagcc aactgtcggc tgaattcttc
      121 gtctcctttt tcttcttcct tatggaatcg agtattttcg agcaacaaaa cttgtccggc
      181 aatgagtgat tttgcgatag cttcggtttt ttcgccgatg cagttttctg caaaaagtac
      241 ttcgcgtccg agtaatttgg atagttccgg tattatattt cttaatgaaa atttctgtac
      301 atcaatagat ccgtcgggga gtagtttctt caatggccgt cccaagtgcg acataaggat
      361 gacagaaaca ccatcaagag acagtaaata ctctattgtg ggcaatgcct ctcttattct
      421 tttgttgtca gtaacattca gatgattgtc gagtggtaca ttgaagtcaa cccttacaag
      481 tattcgtttt ccgactaaat ccagattttg catctccatt gtttttgacc acgaaaatag
      541 taaaaatata ggatacggaa agtcttttgt cgtaaaacaa ttcgtaaaaa tacagttgta
      601 aataccctga cagaatcaaa taggtagtta attgcttcaa ttgcttattt cttgttaaat
      661 atagctgaat aagcagagcc gataaacatt acaagaatac aaccaattgc attgaaccac
      721 agatatccga tatcactgaa gaaatagccc aggatgacta ctgtctctgc aactatggca
      781 cctacaatta cgccattggc acttactttt ttaaggaaaa tggcagaaac cactacaccc
      841 agtatcgtac cataaaacaa tgaacccaga atgttgacag cctgaatcag attttctaaa
      901 ggagctgccc aaagtgcaaa tatcatcaat aggattcccc agccgagtgt taccgttttc
      961 gacattttga ggtaatgttc gtctgttgca tcttttaccc aatttcgttt ataaatatct
     1021 acgataaagg tagtagcaag agcattgatc tcggaggctg tagatgacat cgcagccgag
     1081 aatatcatgg caagtaacag accgatgaca cctaccggaa aataattgag cacatagtac
     1141 atgaaaatgt agtcttcgtc ttttactttg gcagatggat tagcctgact tatcttttcg
     1201 cgtgcctcca ggcggagttc gcggattttt ttgttgtaat cttttagttt ctggctaaaa
     1261 gtttcgtcac ttagattgtc ttttgcaata ttttctgtaa taaatgtttt cctatcattg
     1321 aatactacat caaatttggc ttccaggttt ttgtattcat ttgcaacttc tgtcttcatt
     1381 acgttctgac gattgacttc attgaaatgt aagggtggtt gctggaacaa gaagaaaata
     1441 tataccatca aaccggtgag cagtacagta aactgcatcg gtatttttac cagaccattg
     1501 aacatcaaac caagtctgct ctcggtgagc gtttttccgg taagatagcg ttgtacttgc
     1561 gactggtcgg tgccaaagta ggatagaaac agaaaaaaac tagccaataa tcctgaccag
     1621 atattgtatc tttctttggg gtcaaatttg aagtcaacaa tgttcatttt tccggcactt
     1681 ccggctatgc tgaatgtttc tttgatgcct atgcccgcag gtaattgtga gtaaatgagt
     1741 ataatggcca tgaaaagccc aagtagtata atggccattt gttgtacctg ggttttggat
     1801 actgcattac ttcctccaat aagggtataa acaataacga aactgccaag cataaatatg
     1861 gtgaatgcca tgctccaacc aaacaaagcc gacaaaatga tggcaggagc tataagtgtg
     1921 atactggccg acaaaccgcg caataacaag aacaatacag ctgtaagtgt ccgtactcga
     1981 acatcaaaac gctgctctaa aaattcgtat gccgtgaata ccttgagttt gtagtattga
     2041 ggtagaataa acaccgataa tacgatcatg gccaacggca aaccaaaata gaactgtata
     2101 aagcgcattc cgtcttcgta agcctgccct ggtgtagata aaaatgtgat ggcactcgct
     2161 tgggtagcca ttatagaaaa accaatggtc caccagttga gttcttcatc gcccatgagg
     2221 taggcacgtg tactcgagtt ctttttagtc ttccaaattc cgaaaagtgt aataaataca
     2281 agtgaaccca ataataccga ccagtctatc cagcttatca tttatggttg aatatggatg
     2341 ttaacaaaat gaatactacg agtaacagca gattgaatat gcctacaaac aaataccagc
     2401 ccggccattt gccaaaaacc ggtatttttt ctgaattatt attcttcatt tcttcaattt
     2461 tgttgtacac ccaaagatag catattggcg agcaacttgt aggctcccgg tacgcctaca
     2521 ggcaattgtc tgaataagga taatgaacaa tatacaaagt tgccattacc gtacttgcat
     2581 gttaccaggc tcccgtcaag gtcttgctca cccgggtcat gggttttaag taatttttga
     2641 tatcgtgtat catcggtcgt tataaagtag agacctcgtt cctgcaccca ggcttcaaaa
     2701 tctgcattgg tcagcttatt tggcatgttg aaaatgcggt ggtctttatc aagcatttgc
     2761 attggtgcgt tttcatctgt tactcttcct ctggaaatag taaaaggata agggccgatg
     2821 tccttcgtta ccagtccaaa ggctgtattg tactgacaga tcagcgtacc gccattttct
     2881 acaaatttca tcattatttc gtgtttgtta gccagcatct gtaaggtgtt ataagcgcgt
     2941 acaccggtga ggatgacatc ataattgctt attttttcta attctatttc ttcagccttt
     3001 atgaaatgaa catctatgcc caattgtttc aatgcttcag gtatcttgtc accggctccg
     3061 gtcagatagg cgatttttac cgggctgcat ttaatatcat cgtgtaccaa cctcgaatat
     3121 gaaggtttca acacacgttg ggtcgggatg tggtcataag ctacttcgac cattgagttt
     3181 ctgaattcct gcccctttac tgttgctata aaattgatat cggtctcaga ttttgctgaa
     3241 ggtggactta ttgtaaaact gaacacttgt tcgtcacctt ttttcttaat gatgaacttt
     3301 tggttgttgt tgctgcatat ccagccattg gtggttgtta cacttacagt gccttccata
     3361 ttatcttttg ctgc
//
LOCUS       DAOQQY010000295         3370 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14083_length_3370_cov_5.782202, whole genome shotgun sequence.
ACCESSION   DAOQQY010000295 DAOQQY010000000
VERSION     DAOQQY010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14083_length_3370_cov_5.782202"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..534
                     /locus_tag="PLE23_11415"
     CDS             82..534
                     /locus_tag="PLE23_11415"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HMX83917.1"
                     /translation="MSRIIKTVLVMVSIVFLLSSFTITNNENYSLTVEVENLRNFKGT
                     VQITLYNKDGSIPDEHYKNYFKMEKASIINGKSQCTFSNLPKGNYAVNILHDENNNGK
                     IDKGLILPKEGIGFSNYSSIGLTNRPNFKKANFELAQNKTIKVKVIYM"
     gene            complement(1088..1894)
                     /locus_tag="PLE23_11420"
     CDS             complement(1088..1894)
                     /locus_tag="PLE23_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83918.1"
                     /translation="MLSKILIFICALIAISVLYLSWTIDTDYSTWIIAPVVIGTTALV
                     LAPQIDWWWLKRYPPKTDEQIVRMFEKHLPVFAAQPGPGKAFLLQRTELFMKAFDWTP
                     QGFETIPADVQYVLSFYAAWLTRQRQNFLLKNWEKVILYKNPFPSPAFPRQFHNSEIY
                     HEDHVMIFNIQAVMNGFLRPDLQFPVGLYELARIYCNVFGLNITTADRSITPSNIKSV
                     TGAEWQWVTSGIGLDEVDPDGLAIVCYHFFNHQLCEVLPEVHQRCRQEFG"
     gene            2080..3369
                     /locus_tag="PLE23_11425"
     CDS             2080..3369
                     /locus_tag="PLE23_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMX83919.1"
                     /translation="MFEDLVSKKKKISVTGLGYVGLPIALELARHFSVVGFDVNNDRV
                     GLMKQGIDPSNELDASAFEGVDILFTSDAADLRQAHFHIIAVPTDIDEHKVPNLNPLK
                     GASRSVGRGLKKGDYVVYESTVYPSCTEEDCLPVLEAESSLKLGTDFKLGYSPERIVP
                     GDKEKTLTNILKIVSGSDEEALEEIAKVYGHVIKAGVYKAPSIKVAEAAKVIENTQRD
                     LNISLMNELSIIFDKMGIDTGEVIKAAGTKWNFIKLYPGLVGGHCIGVDPYYLLHKAR
                     ELSVEPQVIAAGRRINDGMPAWIAKKLVQMLISKGKNPGQCKVLMMGVTFKENVSDIR
                     NSKVVNLIHELMQYSVVVHVVDPHASHNEVAHEYKLVMTENISSDYDAVVVTVGHGEY
                     RSLNEQYFKSIMKENAILMDIKGIYNFDATDFTYWKL"
BASE COUNT         1069 a          652 c          796 g          853 t
ORIGIN      
        1 aatggtttat gatgtagtag atagcgtgaa ggggaagtta aaagttaaaa attaagaatt
       61 aaaaattacg aattacgaaa gatgagtaga attattaaaa cagtgttggt aatggtctca
      121 atagtttttt tgttgagttc gtttactatt actaataacg aaaattatag tttaacagta
      181 gaagttgaaa atttgagaaa ttttaaagga actgttcaaa taacattgta caacaaagac
      241 ggttctattc ctgatgaaca ttataaaaat tatttcaaaa tggaaaaagc aagtattata
      301 aatggaaaat ctcaatgtac attttcaaat ttgccaaaag gcaattatgc agtaaacatt
      361 ttacacgatg agaacaataa cggaaagata gataaaggct tgattttacc aaaagaagga
      421 attggatttt caaattattc ttcaatcgga ttaaccaaca gaccgaattt taaaaaagca
      481 aattttgaat tagcccaaaa taagacaatt aaagtgaaag tgatttatat gtaatccaac
      541 acaccgtgta agcacatctt gcattagctt cgctgaactt gggtttcgta caaagccacg
      601 ctaaagccaa aatgcgagaa aagcttacac tacctaccca aaaaaattat ttttccttcc
      661 ctttctaaaa attttaaaac ctgcacctca cagccgacac aacagcaaaa tacagtacat
      721 acaagaatta acaacacgta agacaatacg ccagcctgta acattaagcg gattgaaaaa
      781 gcccgaccac atacgagagg taccttaaaa taccaaatcc ccggatcata aacgaaaggg
      841 gcttttgata tccggtgttc agcgacaccg ggcttggatg ttttttctta ggtggttttg
      901 tgtgtggttt tgaggttttt agtcaacaca ttcgaaattt catactaaat gaaatgcgta
      961 agggatagaa gccggcacag agtaaggcgg atagcccgac ccgtaggggt acgccctgat
     1021 gaagaaaatg taactatgta agatgctgtt aatacgctta tgtggcattc aaaactgatt
     1081 ttagccatca gccgaactct tgccgacagc gttggtgtac ctcgggcagt acctcgcaga
     1141 gctgatggtt gaagaaatgg tagcacacta tggcaagccc gtcggggtcc acctcatcga
     1201 gcccgatacc tgatgtcacc cactgccact cagcaccggt gacagacttg atattggaag
     1261 gtgtgatgct tcggtcggct gtggtgatgt tgaggccgaa cacgttgcaa tatatgcgag
     1321 ccagctcgta taaccctacc gggaactgca ggtcgggtct gagaaatccg ttcataactg
     1381 cttgtatatt gaatatcatg acgtggtctt cgtggtaaat ctcgctgttg tgaaactgtc
     1441 ttgggaaggc gggtgagggg aaaggatttt tgtataggat gaccttctcc cagtttttga
     1501 gcaggaagtt ttgtcgctga cgggtgagcc atgcagcgta gaagctgagc acgtattgta
     1561 catcggccgg aatggtctca aaaccctgtg gagtccagtc gaatgctttc atgaacaact
     1621 ctgtacgctg aagtagaaat gccttgccgg ggccgggctg ggcagcaaag acaggtaggt
     1681 gtttttcgaa catcctaacg atctgctcat ctgttttggg tgggtagcgt ttgagccacc
     1741 accagtctat ctggggtgct aacacaagag ctgtggtgcc aatgaccaca ggtgcgataa
     1801 tccatgtgga gtagtcggtg tctatcgtcc aggaaagata aagtaccgag atggcaatga
     1861 gtgcgcaaat gaaaatcagg atttttgaaa gcatataacg gcgtatgatg ggagtaaaac
     1921 taacaaataa tctacaaata aacaaaaaat cgtatccaat atgtacaatg aaatgcaacg
     1981 aggcttgaat gccaattatt ttacgtaact ttgcagctga attttgaagt gactcttgca
     2041 agattttgaa atcagaattg tgaacaaaaa ctttgttagg tgtttgaaga tttagttagt
     2101 aaaaagaaaa aaatatccgt caccggtctt gggtatgtcg ggctaccgat agcgcttgag
     2161 ctggcaaggc atttttcggt agtaggcttt gatgtcaata atgaccgggt aggccttatg
     2221 aagcaaggca ttgatccgtc gaacgagcta gatgcttcgg cttttgaagg tgttgatata
     2281 ctgttcactt cagatgctgc cgacctcagg caggcacatt tccatatcat agcggtacct
     2341 acggatattg acgaacacaa ggtgcctaac ctcaatccgc tgaaaggtgc ttcgaggagt
     2401 gtcggtaggg gacttaaaaa gggcgactat gtggtctatg agagtacggt gtacccatct
     2461 tgtactgaag aggattgtct gccggtactc gaagccgaaa gtagtttaaa gctcggtacc
     2521 gacttcaaac ttggttactc gcccgaacgc atcgtcccgg gtgacaaaga aaagacactc
     2581 actaatatac tcaaaatagt ctcaggctca gatgaagaag ccctggaaga aatagcaaaa
     2641 gtatatggcc acgtcatcaa agcaggtgta tataaggcac cgagcatcaa ggtagccgaa
     2701 gcagccaagg ttatagagaa cacacagcgc gacctcaaca tatcactaat gaacgagttg
     2761 tctatcattt ttgataaaat gggcatcgac acaggtgaag tcatcaaagc ggccggtact
     2821 aagtggaact tcatcaagct ctatccgggg ctggtaggtg gccactgcat aggtgttgac
     2881 ccatactact tgctacacaa ggcgcgggag ctgtcggtag agccacaggt gatagctgca
     2941 ggtcgccgta tcaatgacgg aatgccggcc tggatagcta agaaactcgt acagatgctc
     3001 ataagcaaag gaaaaaatcc aggacaatgc aaagtgctga tgatgggcgt aacatttaag
     3061 gagaatgtgt cggatatacg caattcgaag gtcgtcaacc tcatccatga gctgatgcag
     3121 tactccgtag tggtacatgt ggtggaccct catgcgtcac acaacgaagt ggcacatgaa
     3181 tacaaactcg tgatgaccga aaacatcagc agcgactatg atgctgtagt agtcacagtc
     3241 gggcatggcg agtaccgctc actgaatgaa caatacttca agagcatcat gaaagaaaac
     3301 gccatactaa tggatataaa aggaatctac aattttgacg ctacagattt tacgtactgg
     3361 aaactttaag
//
LOCUS       DAOQQY010000296         3275 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14743_length_3275_cov_5.681677, whole genome shotgun sequence.
ACCESSION   DAOQQY010000296 DAOQQY010000000
VERSION     DAOQQY010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14743_length_3275_cov_5.681677"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1609)
                     /locus_tag="PLE23_11430"
     CDS             complement(155..1609)
                     /locus_tag="PLE23_11430"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMX83920.1"
                     /translation="MVRLLLSVACICSCFISRAQIIEMGPDKLYKTLQSACQAVTPGD
                     TILIFSGTYNNGDYISNLKGTPNAWISIKAAPGQTVHFTGGTQAFQLSDPEYVIISGL
                     NFSGQSGNGVNIDDAGTFDTPAKYIVFENCHWYKLNASGNSDQLKMSGVENFVIRNCT
                     FNDGSADGSMVDMVGCHDGLFENNVFTNGGSNCIQTKGGSSNITIIRNRFSDGGQRAI
                     NIGGSTDPQFFRPQGANTEASDIFIYSNIFEGANAALAFVGAVNCKAINNTIINPTSW
                     AVRILQENTNAGMLLCSNNTIRNNIFVFSETGKPAFNIGSNTQPASFICSNNLWYNPD
                     NLNWSGPNTPVAEPGIILNQDPVFDDSLYHISENSPAKGNGYPTGEPLKDFFNIAFKN
                     PPSIGAAEYSIKTSTETAFHPRNVEIWPNPFTNKIYVKCYTSTSDRILILNIHGKVVA
                     EVKAGEMAEIDASQWPVGIYFIQNGHFTQKIIKQ"
     gene            complement(1655..2005)
                     /locus_tag="PLE23_11435"
     CDS             complement(1655..2005)
                     /locus_tag="PLE23_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMX83921.1"
                     /translation="MAILITDECINCGACEPECPNNAIYEGGVHWAMIDGTTLSGSYI
                     LEDGKSIEVTAKQAPISDEYYYIVPDKCTECTGFHEEPQCAAVCPVDCCVPDPDRVES
                     KEQLLARKERLHLK"
     gene            complement(2126..>3275)
                     /locus_tag="PLE23_11440"
     CDS             complement(2126..>3275)
                     /locus_tag="PLE23_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX83922.1"
                     /translation="HKLLGRINKADNLNNANQSNSSNTYYGEYQVQREFSNLKLITTA
                     GITETFSNIEAALYSNQKFSIENRAVYLQLDKKFGSRLSFSLGGRYEFNKINVPKNFL
                     RDSFNTEAKPVFRSGLNYQLGRATYLRASFGQGYRFPTIAEKFIRTSFSNFQIFPNDT
                     LRSETGWSAELGLKQGFKIDDWYGFVDVASYWTEYKDMMEFTLVAGSEGIGFRSLNIG
                     NTIIKGFDIGVAAQGNLFGLKTSLFTGYTYLDPKFVHFGAKEMNSSSNKEENILKYRF
                     KHSFKFDGETHYKKFALGASVIYNSDMKAIDNVFNAFIKDVKKFRDEHNKGFTIVDLR
                     VSYQVINSLKSSFIIGNVFNTEYSWRPGRLESMRNLTLRLDYNINKKS"
BASE COUNT          903 a          660 c          657 g         1055 t
ORIGIN      
        1 catcttaaga gttttgatgt gagcgatgac ttgagttttc atccatactt cgaacataca
       61 gtcaccggtc cggcagtcgg tattagctat acacgtggtt ttagtaaaac cgcaccgctt
      121 agtcatcgta tccaattctg ataagcgtct tcacttattg cttgattatt ttttgagtaa
      181 aatgtccgtt ttggataaaa tatattccaa caggccattg agatgcatca atttctgcca
      241 tttcacctgc ctttacttct gcaacgacct ttccgtgtat attgagtatc aatattcgat
      301 cagatgtgga agtataacac tttacataga ttttatttgt aaacggattt ggccagatct
      361 ctacattacg aggatgaaat gctgtttctg ttgaagtctt tatactatat tctgcggcac
      421 cgatggatgg tggatttttg aaagcgatgt taaaaaagtc cttaagaggt tcacctgttg
      481 gataaccatt gcctttggcg ggagaatttt cggaaatatg atacaaactg tcgtcgaaca
      541 caggatcttg gttgagaatg atgccaggtt ctgcaaccgg agtatttggg ccgctccagt
      601 taaggttgtc gggattatac caaaggttgt tcgaacaaat gaagcttgca ggttgcgtgt
      661 tactgcctat attgaatgcc ggttttccgg tttcactaaa tacaaatata ttgtttcgaa
      721 tagtattatt gctgcaaaga agcataccag cattcgtatt ttcctgcagg atacgcactg
      781 cccaagaagt cgggttgatg atggtattgt ttatagcctt acagttgaca gctccaacaa
      841 aggcaagtgc tgcattggca ccttcgaata tatttgaata aatgaagata tcggaagctt
      901 cggtgttggc accctgtggt ctgaagaatt gcggatcagt agagccaccg atgttgatgg
      961 cacgttgacc accgtcggaa aagcgattgc ggatgatggt tatattgctc gaaccacctt
     1021 tcgtctgaat acaattagaa ccaccattgg tgaaaacatt attttcaaac agaccatcat
     1081 gacaacctac catgtccacc attgagccat ctgccgagcc atcattgaac gtacaattgc
     1141 gtataacgaa gttttctact cccgacattt tgagctgatc gctgttgccg gaagcattta
     1201 gcttgtacca gtggcaattc tcaaaaacta tatattttgc aggagtatca aacgtgcccg
     1261 catcatcaat atttacaccg ttgcccgatt gtccagaaaa attgagtccg gaaataataa
     1321 cgtactccgg atctgataac tgaaaagcct gtgtgccacc tgtaaaatga acagtttgtc
     1381 ccggagcagc ttttatactg atccaggcat taggagttcc tttcagattt gaaatataat
     1441 caccattgtt gtaagtgcct gagaatataa gtatggtgtc acccggtgtt acagcctggc
     1501 aggcactttg cagtgttttg tacaatttgt ccggacccat ttctattatt tgtgcccggc
     1561 tgataaagca tgaacatata catgcaactg acaataataa tctgaccatg atacatttaa
     1621 ttatttggtc aaatataaat acttatgatg aaaattactt caagtgaagc ctttctttcc
     1681 ttgccagcaa ttgttctttc gactctacgc ggtcggggtc aggtacacaa caatcgaccg
     1741 ggcatacggc agcacattga ggttcttcgt gaaatccggt acattctgta catttgtcag
     1801 gcactatata gtaatattcg tctgatattg gtgcctgttt ggctgtgact tctatacttt
     1861 ttccatcttc aagtatataa ctgcctgata gggtagtgcc gtctatcatt gcccaatgaa
     1921 caccaccttc gtatattgca ttgttaggac attcaggctc acatgcgccg caattaatac
     1981 attcgtccgt aatcaatatt gccattcaaa tactattttt tccttaaaca acaaaaaaca
     2041 aaataggttt aattaaatcg gacacaaagg tacgatattg ctgttaaccg ggcaaattta
     2101 tctgttaatt caatctacat tggctttaac ttttcttgtt aatgttataa tcaagcctca
     2161 gagtcagatt gcgcatcgat tcaagtctgc cgggtctcca cgaatattct gtattaaaaa
     2221 cattgccgat aatgaaactc gatttgagtg aattgatgac ctggtaagat actcgtagat
     2281 ctacaatggt aaatcctttg ttgtgttcgt ctctgaattt tttcacatct ttaataaagg
     2341 cattaaatac attgtctatt gccttcatat cgctgttata tatgacagat gcgccaaggg
     2401 caaatttctt atagtgtgtt tcaccatcga acttaaatga atgtttaaat ctgtatttta
     2461 aaatgttttc ttctttatta ctggacgaat tcatctcttt cgcaccaaaa tgcacaaact
     2521 ttgggtcgag ataggtgtat ccggtgaata gactggtttt cagcccgaaa agattgccct
     2581 gtgctgccac tccaatgtcg aagcctttga taatggtatt gccaatgttg agcgatctga
     2641 atcctatacc ttcgctacct gctaccaggg tgaattccat catatctttg tattctgtcc
     2701 agtagcttgc cacgtcaaca aagccatacc agtcatcgat tttgaagcct tgcttaaggc
     2761 ctaactcggc actccaacct gtttctgacc taagcgtatc attcgggaat atctggaaat
     2821 tgctgaacga agtacggatg aatttttcag caatggtcgg aaaacggtaa ccttgtccaa
     2881 atgaagctct taggtatgta gcccgcccaa gctgatagtt aagccctgac ctgaatactg
     2941 gttttgcttc cgtgttaaat gagtcgcgca ggaaattctt tggtacattt attttgttga
     3001 attcatatcg tccaccaaga gaaaagctaa ggcgcgatcc gaattttttg tccagttgaa
     3061 gatatacagc acgattttcg atactaaatt tctggttgct gtataatgct gcttcaatat
     3121 ttgagaatgt ttcggtaata ccggcagtag ttatgagttt aagattactg aattctcttt
     3181 gtacctgata ttcgccataa tacgtattgg acgaattcga ctggtttgca ttattgaggt
     3241 tgtcagcttt gtttattctg ccaagtagtt tgtgc
//
LOCUS       DAOQQY010000297         3270 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14780_length_3270_cov_5.335614, whole genome shotgun sequence.
ACCESSION   DAOQQY010000297 DAOQQY010000000
VERSION     DAOQQY010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14780_length_3270_cov_5.335614"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PLE23_11445"
     CDS             complement(<1..348)
                     /locus_tag="PLE23_11445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMX83923.1"
                     /translation="MILTTFYILSAIAIVCALMVVLSKNPVHSVLYLVTCFFAFSGHY
                     ILMNAQFLAVVNIIVYAGAIMVLFLFVVMMLNLNKQNQLDKSNVARLASVIGAGALLL
                     IIVASVKSAELDIL"
     gene            complement(377..922)
                     /locus_tag="PLE23_11450"
     CDS             complement(377..922)
                     /locus_tag="PLE23_11450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HMX83924.1"
                     /translation="MYRSLTAKRKNVSAGQRELTFMERMYLPAILKGMKITIGHLFKK
                     KVSLNYPEVKREFSDFYRGMHVLKRDENGAENCTACGMCALACPAEAITIDAAERKPG
                     EEHLYREEKYAAKYEINMLRCIYCGLCEEACPKDAIYLTERIVPAYYKRKDFIYGKDI
                     LVEDINNRVDITKRKQPIKKA"
     gene            complement(927..1964)
                     /gene="nuoH"
                     /locus_tag="PLE23_11455"
     CDS             complement(927..1964)
                     /gene="nuoH"
                     /locus_tag="PLE23_11455"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMX83925.1"
                     /translation="MLSTYLIVKIVIILVLFLVTLGMAAYSTYGERKVAAFLQDRLGP
                     NRAGPFGLLQPLADGVKMFMKEEIIPNTSDKAIFILGPSIFMVTALLTSAVIPWGPDI
                     HIDGQAYPLQVADINIALLYLFGIVSLGVYGIMLGGWASNNKYSLLGAVRASSQMISY
                     ELAMGLSIVGILMYTGSLSLRDIVAQQQGFHWNVFYQPVGFVIFLICAFAEANRAPFD
                     LAECETELIGGFHTEYSSMKLGLFLFAEYIHMFVSSAVIACLFFGGYDFPFIDKLGLS
                     PNVFAVVGVVILFAKIIFFIFFFMWVRWTIPRFRYDQLMKLGWQILIPLGLANIFITG
                     GWLTLKNYLNW"
     gene            complement(1969..2955)
                     /locus_tag="PLE23_11460"
     CDS             complement(1969..2955)
                     /locus_tag="PLE23_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMX83926.1"
                     /translation="MAKVTIDDIMIEVPDGITILEAARMIGGHVVPPTMCYYSSLETS
                     GGYCRTCIVKVEKGSEKDPRPMPKPVASCRTTVQDGMVVRNATSPEIQKARKGIVEFL
                     LINHPLDCPVCDQAGECFLQDLSFEHGDEFSRFDFKKRTFDLIDIGDYIKLHMTRCIM
                     CYRCVKVADQLTEERLHGVLNRGDASEIGTYIQQAVDNDFSGNMIDACPVGALTDRTF
                     RFKSRVWFTKPMYAHRDCDKCCGKVTLWYQGEEVLRVTGRKNEFGEIEEFICNTCRFD
                     KKHTQDWVIEGPAKISRHSVIVQNHNELALPKDEVQILPDLPGSTSHKKIES"
     gene            complement(2975..>3270)
                     /locus_tag="PLE23_11465"
     CDS             complement(2975..>3270)
                     /locus_tag="PLE23_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HMX83927.1"
                     /translation="HHESCGQCSPCREGTRWMDQILSNILKGNGKMKDIELLWDIQGN
                     IEGKTICPLGDAAAWPVASAIRHFRKEFEEFIENPAAVKDVKHYYSVNNISEA"
BASE COUNT          940 a          715 c          612 g         1003 t
ORIGIN      
        1 aagtatgtcg agttcggcag attttacaga agctactatt attaacagaa gagcaccggc
       61 accgatgaca cttgctaacc ttgctacatt acttttgtcg agctgatttt gcttattgag
      121 gttgagcatc attactacga acagaaacaa caccattatg gcaccagcat atacaataat
      181 attcaccact gcaagaaact gcgcattcat taatatgtaa tgtccagaaa atgcaaagaa
      241 gcaagttacc agatacaata cgctatgtac cggatttttc gaaagtacca ccatgagcgc
      301 gcatacaatg gcgatagctg ataatatata aaatgtagta agtatcattg tttgcttagt
      361 ttgattaaat atgaaattaa gcttttttga tcggctgttt gcgtttggta atatctaccc
      421 ggttattgat atcttctacc aaaatatctt taccgtaaat aaaatctttt cttttataat
      481 atgcaggtac tattcgttct gtcaggtata tagcatcttt aggacaagct tcttcgcaca
      541 gaccacaata tatacaccta agcatattga tttcgtattt tgctgcgtat ttttcctctc
      601 tgtatagatg ttcttcgccc ggttttctct cagcggcatc tattgtaatt gcttcagcag
      661 ggcaggcgag agcacacata ccgcaagctg tacagttttc agctccgttt tcatctcttt
      721 taagcacatg catacctctg taaaaatcgg agaactctcg cttgacctcc ggataattga
      781 gcgatacttt ctttttgaac aagtgaccga tcgttatttt catgccttta agaatggcgg
      841 gaagatacat tcgttccatg aatgtaagtt ctcgttgtcc ggcagacaca ttttttcttt
      901 tagctgtaag tgatctgtac atgatattac cagtttaggt agttttttaa ggttaaccaa
      961 cctccggtta taaaaatatt agcaagtccc agtggtatga gtatctgcca accgagtttc
     1021 atcaactgat cgtatctgaa acgcggtata gtccatctta cccacatgaa gaagaaaata
     1081 aaaaatatga ttttggcaaa cagtattacc acaccaacta cggcaaacac attgggtgat
     1141 agtccgagtt tgtcaataaa aggaaagtcg tatccgccga aaaacaaaca agcgataaca
     1201 gctgatgaaa caaacatgtg gatgtattct gcaaacaaga ataagccgag tttcatactt
     1261 gaatattctg tgtggaaacc acctattagc tcagtttcgc attctgcaag gtcaaatggt
     1321 gctctgttgg cttcagcaaa tgcacaaatt aggaaaatta caaatccaac cggctggtaa
     1381 aacacattcc aatgaaagcc ctgctgctgt gctactatat cacgcaaact caacgaacct
     1441 gtgtacatca gaatgcctac tattgaaaga cccatagcaa gttcgtaact gatcatctga
     1501 gagcttgccc ttactgcgcc gagtagtgaa tatttgttgt tggatgccca gccacctaac
     1561 atgataccat atacaccaag agatactatc ccaaaaagat aaagcaatgc tatattaata
     1621 tctgctactt gaagcggata agcctgacca tcgatatgaa tgtccgggcc ccatgggata
     1681 acggcactgg taagcaaagc cgtaaccata aatatagacg ggccaagtat aaaaatggct
     1741 ttgtctgatg tattaggtat gatttcttcc ttcatgaaca tcttaacacc atctgccaaa
     1801 ggctgcaata agccgaaagg tcctgctcgg ttagggccaa gtctgtcttg aagaaaagcg
     1861 gcaactttac gttcaccgta tgttgaatat gctgccatac ctagtgtcac caagaacaac
     1921 acaaggataa taactatttt gactataaga taggtactca acataacttt agctttctat
     1981 ttttttatgt gaagtggatc cgggaaggtc tggtaatatc tgtacttcat ccttaggtaa
     2041 agccagttca ttgtggttct gtacgattac tgaatgacgg cttatctttg ccgggccttc
     2101 aattacccag tcttgtgtgt gttttttgtc gaaacggcaa gtgttacaga taaattcttc
     2161 tatttcacca aattcatttt ttcttcctgt tactcgcaac acttcttctc cttgatacca
     2221 aagggttact ttaccacaac acttgtcgca atcacggtgg gcatacattg gtttagtaaa
     2281 ccatactctg ctcttaaagc ggaaggttcg gtctgtaagt gctcctacgg ggcaggcatc
     2341 tatcatattc cctgaaaaat cattatcaac tgcctgttgt atgtatgtac caatttctga
     2401 agcatcgcca cggttcaaaa cgccatgtag tctttcttcg gtaagctggt cagctacttt
     2461 tacacaacgg tagcacataa tgcaccttgt catgtgaagt ttgatatagt cacctatatc
     2521 tatcagatca aaagttcttt tcttgaagtc gaaccggctg aattcatcac cgtgttcaaa
     2581 actcaggtct tgtaggaagc attcacctgc ctgatcgcat accgggcagt caagcgggtg
     2641 attgatgagc aaaaactcaa cgattccctt tctggctttt tgtatttcag gtgatgtagc
     2701 attccgcaca accataccgt cttgtacagt agtacgacaa gaagctactg gtttgggcat
     2761 tggtcttgga tctttttctg atcctttttc aacctttacg atgcatgtac gacaataacc
     2821 gccactggtc tccagcgaac tgtagtagca catagtaggt ggaacgacgt gtccgcctat
     2881 cattcgggct gcctccagaa tggttatccc atccggtact tcaatcatta tatcatctat
     2941 cgttacttta gccatgacga tttattaatt tttattaagc ctcagagatg ttgttgacac
     3001 tgtagtaatg ttttacatct tttacagcag ccggattttc aataaattct tcaaattctt
     3061 tcctaaaatg tcttatagca cttgctacag gccacgcagc tgcatcgcca agtgggcata
     3121 ttgtcttccc ctcaatgtta ccctgaatat cccaaagcaa ttcgatgtcc ttcatttttc
     3181 cgttgccttt cagtatgtta gataaaatct gatccatcca cctggtacct tcacgacatg
     3241 gcgaacactg tccacaactc tcatgatgat
//
LOCUS       DAOQQY010000298         3262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14829_length_3262_cov_6.170876, whole genome shotgun sequence.
ACCESSION   DAOQQY010000298 DAOQQY010000000
VERSION     DAOQQY010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14829_length_3262_cov_6.170876"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLE23_11470"
     CDS             <1..307
                     /locus_tag="PLE23_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HMX83928.1"
                     /translation="YEFDLRHHIIADYEVANWYTSTCPGSRFTHQLIVARAEGNRRFT
                     LTDLIYSEYNHGKRIKRTKLDNYPDIQHLLERNFLIDLSGLTGLQELVEHKLKSEIS"
     gene            473..1930
                     /locus_tag="PLE23_11475"
     CDS             473..1930
                     /locus_tag="PLE23_11475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMX83929.1"
                     /translation="MPIRQLTKQFPFWLVTLSVVFGLTLPILIQEGMFQDAVLYSAVS
                     HNLAIGFGNFWFPQYSTLNLEGIPSFHEQPPLVFGIQALFYKLLGDSMYVERFYTLLM
                     IVLHILLIRYLWKNIFPDNERYMAVGWLPVLFWILIPVGYWSFRNNMIENTVSVFTLC
                     SVIVSYKNVQSDGKNILLWLASGFFIFLATFSKGVPGFFPITFPFIYWLVSRKISFRE
                     CALYTMILTAVPLIMYGVFLLFPQSRESLSIYFYERLVRRVNSMPTADYRLEIVWRLF
                     TELIPVMLFVILSYLLSGIKMLRSIFPKQKADFLLFLLVGLSGALPLTLTMVQKGWYM
                     VPSFPFLGIAFAVLVVPVISSTIQRIDMNQWKYKLFLTVSVLLFVSMTIFTISQKGKI
                     SREQDVISDVYQIGSVVPRFSTLTVPAKMYDQYDFVLQGFLVRYFNISISPYKQYEYF
                     LKEKTMDTTIPQNYQKLDLKLSKHELYKTVGLPSQ"
     gene            complement(2215..>3262)
                     /locus_tag="PLE23_11480"
     CDS             complement(2215..>3262)
                     /locus_tag="PLE23_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83930.1"
                     /translation="SEGDFWKKLNDGQLDNSAARIYNDFYHNPYSNFNDVAQDKNLTI
                     GKVFVNEGAEGVTKGAEVIIEVTKVAAPGLGKGIELVEKGQEYAEKIETVYKDPKQFI
                     KNEVKEQIADYLGGFVDIDGAVDAGKLSEAAGAAIKILTDYTLGSDDPADWVKSAIDY
                     GLAKILDGNTADKANVAIAVKADNNKNQTGPNVVISVDPTKDDKDLEDVIDIMLPGGD
                     WVISPVNIKGYTDKIIAEVIEEIASVIPVNTDPNAPHVNGQYALSVWISPADPGPGEG
                     VTVTAKISPAKSGEDIYFSITGTDGYSSSSTNKTDNEGKATFYIPGGAGGVRDEVHIK
                     IVSTGTERVIVYTF"
BASE COUNT          855 a          691 c          660 g         1056 t
ORIGIN      
        1 ctatgagttt gacctgagac atcacatcat cgctgactat gaagtggcca actggtacac
       61 ctctacatgt ccgggttcgc gatttactca tcagctgata gtggccagag cagaaggcaa
      121 ccggcgattc acattaactg accttatata ctctgaatac aaccatggca aacggattaa
      181 aagaaccaaa ctggacaatt accctgatat ccaacatttg cttgaacgca actttctgat
      241 cgacctgagt gggttgacag gcctgcagga acttgtggag cacaaactaa agagcgaaat
      301 ctcttgattg aagaatactt gtaatctgta gaaagtatat tatggtaaaa tcggtagaat
      361 gtcagaatta atatctgatt cctgccggga aattgtattt ttgcccgaca tgaagcgcat
      421 ctgattgccc tgaaattttt tgtactgttt ttataaaata ttcaaaacga aaatgccgat
      481 tcgccaacta acgaaacaat ttccattctg gcttgtgaca ctcagtgtag tgtttggtct
      541 tacattaccg atactcatac aggaagggat gtttcaggat gcagtgctgt acagtgcagt
      601 gtcgcacaat ctggctattg gatttggcaa cttctggttt ccacaataca gtacacttaa
      661 tcttgaaggc attccatcat ttcacgaaca gccacctttg gtattcggca ttcaggcttt
      721 gttttataaa ttgctgggcg acagtatgta tgttgaaaga ttttatacct tgttgatgat
      781 cgtacttcac attctgctca tcaggtatct gtggaaaaat atctttcccg acaatgaacg
      841 ctacatggca gtgggctggc tgccggtact attctggata ctgatacctg taggctactg
      901 gtcgttcaga aacaatatga ttgaaaatac agtaagtgtc tttacgttat gttcggtgat
      961 tgtcagttat aaaaatgtac agtctgacgg gaagaatatt ctgctctggc ttgcatccgg
     1021 tttcttcatt tttctggcta cattcagcaa aggtgttcca ggatttttcc cgataacatt
     1081 tccattcatt tactggctgg taagccggaa aatcagtttc cgggaatgtg ctctttacac
     1141 tatgatactt acagctgttc cgttgattat gtatggcgtt ttcctacttt ttccgcagag
     1201 cagagagagc ctgagcatat acttttacga aaggttggta cgtagggtca atagtatgcc
     1261 tacagccgat tacaggctag aaattgtgtg gcgattgttt accgaactca ttcctgtaat
     1321 gctttttgtc atactgagct acctgttgtc aggtataaaa atgttgcgta gcattttccc
     1381 gaaacagaaa gccgattttc tgctgttttt gttagtcggc ctgtcaggtg cattgccact
     1441 cacgcttacc atggtacaga agggttggta catggtgcct tcatttccgt ttcttggtat
     1501 tgcctttgcc gttcttgtag ttccggtcat ttcatcaact attcaaagaa ttgatatgaa
     1561 ccaatggaag tacaagttat ttcttacggt ttctgtgctt ctttttgtat caatgacgat
     1621 tttcaccatt agccaaaagg gaaaaataag cagagagcaa gacgtgataa gtgatgtata
     1681 tcaaataggt agtgtagtac cccgtttttc gacgctcacc gtgcctgcta aaatgtatga
     1741 ccagtacgac tttgtacttc agggtttttt ggtcaggtat ttcaacatca gtatcagccc
     1801 ttacaagcag tacgaatatt ttctgaaaga aaagacaatg gatacgacaa ttccgcaaaa
     1861 ctaccaaaag cttgatctaa aattgagtaa acatgaactc tacaaaacag ttggtttgcc
     1921 gtctcaataa ttgttaaaag ttatttgctt tttttgcaat tgcattacga gaaacttgtt
     1981 acccgatgaa tgaaccggga tttttaaatg cccatcacac gactttgatt tttggggttt
     2041 tactcaccct tcatccgtgt cggatcttgt atatgtgaat gattttgaag caaggatgaa
     2101 aacgtttcat tattcagtag tatattaagc taaaaaatag tggcagcgat agaactttta
     2161 ccgccgccac ttttgatcga tgacggttta ttgagaaaaa tttaggtaaa cgtatcagaa
     2221 ggtgtaaaca ataactctct ctgtgccggt agatacgatt ttgatgtgta cctcatccct
     2281 tacaccgcct gctccaccgg gtatatagaa tgtcgctttg ccttcattgt ccgttttatt
     2341 ggttgaactg ctggagtaac cgtcagtgcc tgtgatgctg aagtagatgt cttcgccact
     2401 cttggccgga gatattttcg ctgttacagt cactccttct cccggtcctg ggtcagccgg
     2461 gctaatccaa actgagagtg cgtattgtcc atttacatga ggtgcatttg ggtcggtgtt
     2521 taccggaatg acagaagcaa tttcttcaat gacttcagcg attatcttgt ctgtataacc
     2581 cttgatattc accggtgaaa taacccaatc accaccaggg agcataatgt cgataacatc
     2641 ttccaagtcc ttgtcatctt tggttgggtc aactgatatc accacatttg gtccggtttg
     2701 atttttgttg ttatctgctt ttacagctat ggctacattt gctttgtctg ccgtattgcc
     2761 atccagtatc ttggccaacc catagtcgat agcgcttttt acccaatcgg caggatcatc
     2821 agaacccaat gtatagtctg tcaatatttt gattgctgca ccggcagctt cactaagttt
     2881 gcctgcatca acagcaccat caatgtcaac aaaaccacca agatagtccg ctatctgctc
     2941 ttttacctca tttttgataa actgcttagg gtctttatat actgtttcta ttttttcagc
     3001 atactcctgc cctttttcta ctagttcaat ccctttgcca aggccaggag ctgccacttt
     3061 ggttacttct attatcactt ctgccccttt ggtcacgccc tctgcacctt catttacaaa
     3121 caccttaccg attgtcagat ttttatcttg agctacatcg ttaaagtttg agtaagggtt
     3181 gtggtagaaa tcgttgtaaa tcctggctgc tgagttatct agctgcccgt cattcagctt
     3241 tttccaaaaa tcaccttcag aa
//
LOCUS       DAOQQY010000299         3242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14962_length_3242_cov_7.321619, whole genome shotgun sequence.
ACCESSION   DAOQQY010000299 DAOQQY010000000
VERSION     DAOQQY010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14962_length_3242_cov_7.321619"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="PLE23_11485"
     CDS             <1..1115
                     /locus_tag="PLE23_11485"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMX83931.1"
                     /translation="NSIRQSGIDLMAARKDSEQLMENISLTIATQYLTILLAQEAVEI
                     AKKRLETSKKQLDQTDKLISAGSLPKNERLMIQATVATDEENLLIQQNNVDLGYLDLK
                     NLLNLDPATDMTIKKPEVDQFLIDNTLLTNVEELFKKSADRNPGVAANNLKIESARIG
                     KKIASSTGLPSLDIFGSFNTNYSSSILDLLHPDLSNLKEELSNPTKVNINGMDVFIAT
                     YNKKGISYPSKPYFSQLQDNLGQSLGLSLRIPIYNAGITHYNKQRADLAIKLAELNSD
                     LTKQQLKNQIYRAVTDYKAARLRVEVAQRENEAAQGSFVNLQKKYDAGASNSLELITA
                     KNNADAAEDNLLTARYSLIFRTKVLEYYMGQPIQLN"
     gene            1130..2521
                     /locus_tag="PLE23_11490"
     CDS             1130..2521
                     /locus_tag="PLE23_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMX83932.1"
                     /translation="MAIKKNRKWLWIALASIGVLVAFAVYKAKSRPNGEKVYTDEVVK
                     REIREIVSASGKIYPKTEVKISSDVSGEIVALYVKEGDTVKAGQLLAKIDPDAYVSQV
                     DRGVASLNVSKSQIENSRKVLEAAKAQRDQVSSQVENARLVFERNKKLHNDGVISDVE
                     FQASQSSYKALEANLKSANASIAQAEENVRTSTYSMKGSEATLKEVQKSLSRTSILAP
                     TSGIISKLNVEKGERVVGTIQMTGTEMMRIANLNIMEVQAEVSENDILRVQLGNDVNI
                     EVDAYPNKKFKGKVTEISNSSTTAGLESSSASLATDKITNYIVKIIIDQSSYSDLIVP
                     GKGFPFRPGMSASVEITTNIVKDALCVPIQAVTTREIGKDTTNHTNPKADDLKEVVFV
                     PTNDTARMVEVSTGIQDEAYIQVLSGIKPGEKVVAGPYSVVSRTLKSGKKIVVVKKDE
                     INSKSKKPESTED"
     gene            2518..3129
                     /locus_tag="PLE23_11495"
     CDS             2518..3129
                     /locus_tag="PLE23_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HMX83933.1"
                     /translation="MTHKPDCLIIFVKNPVAGKVKSRIAATTGTDKALEIYLQLQEVI
                     RSTVCQLTDIQKVVYYSDEICHTDEWENDVFVKRKQKGANLGMKMYNAFAEYLRHHRK
                     VVLIGSDCPYITVNIIRNAFSALSDSGVVIGPAVDGGYYLIGMKVPHNKIFENINWST
                     EHVFAQTKERIDKLKVSLSLVEKLSDIDTEQDWLNWTATITNN"
BASE COUNT         1146 a          669 c          667 g          760 t
ORIGIN      
        1 ccaattccat taggcaatct ggtatagacc tgatggctgc ccggaaagac tccgaacaac
       61 tgatggaaaa catttcgcta accattgcta cccagtatct cacaatattg cttgctcagg
      121 aagctgtaga aattgcaaaa aaacgactcg aaacctccaa aaaacagctc gatcagactg
      181 acaaactcat aagtgcaggt agcttgccaa aaaacgaaag actcatgata caggctaccg
      241 tagccactga tgaagaaaat ttgctcatac aacaaaacaa tgtcgatctt gggtatctcg
      301 acctgaaaaa tcttctgaac cttgacccag ctacggatat gacgattaaa aaacccgaag
      361 tagatcagtt tttaattgat aataccttac tgacaaatgt cgaagaacta ttcaagaaat
      421 cagctgatag aaatcccgga gtagctgcta acaatctaaa aattgaaagt gccagaatag
      481 ggaagaaaat agcatcctcc accggattgc caagccttga tatatttggt agcttcaaca
      541 ccaactattc gagctccatt ttagaccttc tgcatcccga tttatcaaat ctgaaagaag
      601 aactatctaa ccctaccaaa gtgaacatca atggcatgga tgtattcatt gctacctaca
      661 acaaaaaagg tatatcttat ccgtccaaac catatttcag ccagctacag gacaacctcg
      721 gtcaatcact tggtctgagc ctaaggatac ctatctacaa tgcaggtatc acacactaca
      781 acaagcagcg tgcagaccta gccatcaaac tggcagaact gaacagtgac cttacgaaac
      841 agcaactcaa aaaccaaatt tacagagcag ttactgatta caaagcagcc agattgaggg
      901 tggaggtagc acaaagagaa aacgaagcag cacagggttc attcgtaaat cttcaaaaaa
      961 aatacgatgc aggtgcatcc aattcgcttg aattgataac agctaaaaat aatgcagacg
     1021 ctgccgaaga caatctgctg acagcaagat attcattaat tttcaggaca aaagtacttg
     1081 aatattatat gggtcaaccc attcaactca attaaagaaa ataggtatta tggcaattaa
     1141 aaagaataga aaatggttgt ggatagcact tgcatctatc ggtgtgctgg tggcttttgc
     1201 tgtttataaa gcaaaatctc gtccaaatgg tgaaaaagtc tataccgatg aggtggtaaa
     1261 acgtgagatt cgcgaaatag tctctgcaag cggaaaaata tatccaaaaa cagaggtgaa
     1321 aataagctca gatgtgtcgg gtgagattgt agcactctat gtcaaagaag gagatactgt
     1381 aaaagcaggt caactgttgg ctaaaattga cccagacgca tacgtatcgc aggtagatcg
     1441 gggtgtcgct tcgctcaatg tatcaaaatc gcaaatcgaa aactcaagga aagttctcga
     1501 agctgccaaa gcccaacgcg accaggtatc aagccaggta gaaaatgcac ggctggtatt
     1561 cgaaagaaat aaaaaactgc acaacgatgg tgtcatctct gatgttgaat tccaggcgag
     1621 tcagtcatcg tacaaggcac tggaggccaa cctcaaatca gccaatgcct cgattgctca
     1681 ggcagaagaa aatgttagga cttctaccta ctctatgaaa ggttcggaag caactctgaa
     1741 ggaagttcaa aaaagcctaa gccgtacttc catccttgca cctacctcag gcatcatcag
     1801 caaactcaat gtagaaaaag gtgaaagagt ggtaggtacg atacagatga caggtactga
     1861 aatgatgcga atagccaacc tcaatatcat ggaagtgcag gcagaagtaa gcgaaaacga
     1921 cattctccgt gtgcagcttg gcaatgatgt gaacatagaa gtagatgcct atccaaacaa
     1981 aaaattcaaa ggaaaagtaa ctgaaataag caattcgtcc actaccgcag ggctcgaatc
     2041 atcctcagct tctctcgcta ctgataagat taccaattat atagtgaaaa tcatcattga
     2101 tcaatcttcc tattccgacc tgatcgttcc aggtaaaggt ttcccattca ggcctggcat
     2161 gtcagcatct gtcgaaatca ctaccaatat agtaaaagac gcactttgtg taccgattca
     2221 ggctgtaact acaagggaga taggaaaaga tacaacaaac cataccaacc ctaaggcaga
     2281 cgacctgaag gaagtggtat ttgtacccac caatgacact gcaagaatgg tggaagtaag
     2341 cacaggcata caggacgaag cctatataca agtactcagt ggcataaaac ctggtgaaaa
     2401 ggtagtagcc ggaccatatt ctgtagtatc ccgtacactt aagagtggaa aaaaaatagt
     2461 cgttgtaaaa aaagatgaaa ttaattcgaa gtccaaaaaa cctgaaagta cagaagattg
     2521 acacacaaac cagattgtct gattattttt gttaaaaacc ctgtagccgg taaagttaag
     2581 tcgcgtattg cagctaccac cggtacagat aaagcattgg aaatctacct gcaactacag
     2641 gaagtgataa gatcaacagt ctgtcaattg accgatatac agaaagtcgt ttattattct
     2701 gatgaaatat gccatacgga cgaatgggaa aacgatgtgt ttgtaaaaag gaaacaaaaa
     2761 ggtgccaatt tgggaatgaa aatgtacaac gcattcgcag aatatctccg gcatcaccgc
     2821 aaagtagtac tgatagggag cgattgtccg tatatcaccg ttaacattat ccgaaatgca
     2881 ttttcagctc tttcagattc tggtgtggtg ataggtccgg ctgtggatgg tggttactat
     2941 ctcattggca tgaaagtacc tcacaataaa attttcgaaa atattaactg gagcaccgaa
     3001 catgtatttg ctcagacaaa agaacggatt gataagctga aggtaagtct gtctttggtt
     3061 gagaaattat ccgacattga tacagaacaa gactggctca actggacagc aaccatcacc
     3121 aataattgag agaaattagt gttgataata aaaaaagcac ttgccacaga caagtgcttt
     3181 taatattgtg ggcccaccag gacttgaacc tgggaccccc tgattatgag tcaggtgcta
     3241 ct
//
LOCUS       DAOQQY010000300         3240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14986_length_3240_cov_5.317111, whole genome shotgun sequence.
ACCESSION   DAOQQY010000300 DAOQQY010000000
VERSION     DAOQQY010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14986_length_3240_cov_5.317111"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="PLE23_11500"
     CDS             complement(<1..1506)
                     /locus_tag="PLE23_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HMX83934.1"
                     /translation="MSQSRFNAIDAIMHRTEFDTEYVSFPSENISSFFGSNVFNDESM
                     KSYLAEDAYLNVKATIQSGGKLSMELADIVADAMKKWAMDKGATHYAHWFQPWTGRTA
                     EKHDSFFTLEGQNAIEKFEGKELAQQEPDGSSFPGGGLRSTFEARGYTAWDPSSPAFI
                     IEIGDGKTLCIPTILVTYGGEALDFKLPLLRSLTALEEAALPVAKWFEPDATKIITTL
                     GWEQEYFLIDEALFNARPDLMMTGRTIIGKHSPRGQQLEDHYFGSIPDRVFNYMRDLE
                     TECHKLGIPVRTRHNEVAPAQYELAPMYEEANIAVDHNQLLMDVMERVAKRHKLKVLF
                     HEKPFAGVNGSGKHNNWSLATNTGKVLLAPGSNPAKNLSFLTFFINTIMAVSEHADLL
                     RASIASISNDHRLGANEAPPAIVSVFIGHGLTEVLDEIESGKASKSEVKKVMDLISKI
                     PNLIQDNTDRNRTSPFAFTGNKFEIRMAGSSANCAAPITALSTMMANQLTKF"
     gene            1660..>3240
                     /locus_tag="PLE23_11505"
     CDS             1660..>3240
                     /locus_tag="PLE23_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMX83935.1"
                     /translation="MKQIFFALFCLFLLPDSACGQTVETISENDFINAIIEDFLSDND
                     ELNVDPVMLYDQLNELKNHPININKAGFEELQQLFFIPDNILQDILTYRSEEGDFISV
                     YELQAVPTLGVDDIRKMIPFITIGEKASRFNVPIPEMLLKGQNTVFVKWETLAEEQVG
                     YSEERKISGSSYYLGDRYKLFLRYRHQYSNKLSYGFTAEKDPGEEFFQGSNKKTGFDF
                     YSFHAFLKNYSPRLKAIALGDYAISLGQGLITNYGFGYGKSSFATLIKKGGPVLLPYT
                     STLENNFFRGIAATVQLSKRFECTAFASHRNVDANLGEPDSLIDYDFGFTSLQASGFH
                     RTQAEIDDKNAIKSTDAGLNLKYNYHNLSISLNGLYTEFNKVYIPQKRLDNLFAFSGK
                     SLSNISFDYAYIYRNFYFFGETARSDNGALATVNGVLITLDRRVDLALFHRYLPVDYQ
                     SLNPRVFAEGSTGSNENGFYLGAKFYPARGWTIDAYFDVWKNPWLRFNVASPSVGREY
                     LAKIQYSIKRKFDIYIQYK"
BASE COUNT          916 a          644 c          641 g         1039 t
ORIGIN      
        1 aaactttgtt aactgattgg ccatcatcgt actgagtgcg gtgattggtg cagcacaatt
       61 agcacttgaa cctgccattc ttatttcgaa tttattacct gtaaatgcga aaggcgaagt
      121 acggtttctg tctgtattat cctgaatgag gtttggtatt tttgaaatca ggtccatcac
      181 cttctttacc tcagactttg atgctttgcc agactctatt tcgtctaaca cttctgtaag
      241 tccgtgacct atgaatactg atactattgc aggtggagct tcattggcac caagcctgtg
      301 gtcgttactg atagaagcga tggaagcacg cagtaggtcg gcatgctcac ttactgccat
      361 gattgtatta atgaaaaagg tcagaaagct aagattttta gccggattag agccaggcgc
      421 caataggact ttacctgtat tagtagctag tgaccaattg ttgtgttttc cgctaccatt
      481 cacacctgca aaaggttttt catgaaacaa aaccttgagt ttgtgccttt tggccacacg
      541 ttccatcaca tccatcaaca actgattatg gtcaacagca atattggcct cttcgtacat
      601 gggtgctagt tcgtattgtg caggtgctac ttcgttatgt cgtgtgcgaa cgggtatgcc
      661 gagtttgtgg cattctgttt cgagatcacg catatagtta aatactctat ctggtataga
      721 accaaaataa tgatcttcga gctgttgccc tctaggtgaa tgtttgccaa taatggtacg
      781 gcctgtcatc atcaggtcgg gccttgcatt aaaaagtgct tcatctatca ggaaatattc
      841 ctgttcccag cctaaggtgg tgataatttt cgtagcatcc ggctcgaacc atttggctac
      901 cggtaatgct gcttcttcaa gtgcagttaa tgaacgcagt aagggcaatt taaaatcgag
      961 tgcttcgcca ccgtaggtta caagtattgt aggtatgcat agtgtctttc catcccctat
     1021 ttcaataata aaagcaggtg atgatggatc ccaagcagta taaccacgtg cctcgaaggt
     1081 tgaacggaga ccaccgccag gaaaacttga tccgtcgggt tcttgctgag caagttcttt
     1141 cccttcaaat ttctcaatag cattctggcc ttcaagcgtg aagaatgaat cgtgcttttc
     1201 ggctgtacgg ccggtccagg gttgaaacca gtgtgcgtaa tgtgtagcac ctttatccat
     1261 tgcccatttc ttcatggcat ctgctactat atctgccagc tccatgctta actttccacc
     1321 agactggatg gttgccttta cattgagata ggcatcttca gcaaggtagc ttttcatcga
     1381 ttcgtcattg aatacattgc tgccgaagaa tgaagatatg ttttctgatg gaaatgaaac
     1441 atattcagta tcgaattcgg tacgatgcat aatggcatca atagcattaa aacgtgattg
     1501 actcatttgt ttgttgttat tttattgggt gaaatgcgct gcaaaattaa tttattctta
     1561 tgttttgact ataatttttt tatttttata attgtgaaat tgaattattt ttgctttatc
     1621 tttcatcaat atatgaataa caaatatttt tctgctttta tgaagcaaat tttttttgca
     1681 cttttttgtt tgtttttgct acctgactct gcctgtggcc agacagttga aactatttct
     1741 gaaaatgact ttataaatgc tatcattgaa gactttttat ctgacaacga tgaactgaat
     1801 gttgacccgg tgatgttgta cgaccagtta aatgaattaa aaaatcaccc gattaacatc
     1861 aacaaagccg gatttgaaga attgcagcaa ctatttttta tacctgacaa tattcttcag
     1921 gatattttga cttacaggtc agaagaaggc gattttatat cggtttatga gttacaagct
     1981 gttccgacac ttggtgtaga tgatatacgc aaaatgatac ctttcatcac cataggtgaa
     2041 aaagcatcaa gattcaatgt gccaattccg gaaatgttgc taaaaggtca aaatacagta
     2101 tttgtaaaat gggaaacttt ggcagaagaa caggtcggat attctgaaga acgaaaaatc
     2161 tccggctcct cctattattt gggtgatcgt tataaactct tccttcgtta ccgtcatcaa
     2221 tattctaata aactatctta cggttttaca gctgaaaaag atccgggtga agaattcttc
     2281 caaggttcaa acaaaaaaac cggtttcgat ttttacagct tccatgcttt tctcaagaac
     2341 tattcaccac gactaaaagc catcgcactt ggagattatg ctatatcttt gggtcaaggt
     2401 cttataacca attatggctt tggatatggg aaaagttcgt tcgctacact catcaaaaaa
     2461 ggtggccctg tactgttacc atacacatct acacttgaaa acaatttctt ccgaggaata
     2521 gcggctactg tacagctgag caaacgattc gagtgtactg cctttgcttc gcacagaaat
     2581 gttgatgcaa acctcggcga accagattca ctcattgatt atgattttgg atttacttca
     2641 ttgcaggcct ccggctttca ccgaactcaa gccgaaatcg atgataagaa tgcaataaaa
     2701 tcaactgatg ccggattaaa cctaaagtat aattaccaca atctaagcat ttcattaaat
     2761 ggactttata ctgaatttaa caaagtgtat attcctcaaa aaagattgga taatctcttt
     2821 gccttttcag gaaaatcgtt atccaacatc agctttgatt atgcttatat ataccgaaat
     2881 ttttattttt ttggagaaac ggcacgatct gacaacggag ctttagctac tgtcaatggt
     2941 gtattgatta ctctcgaccg ccgggtggat cttgctttgt ttcatcgata tctacccgtc
     3001 gattaccaat ctttgaatcc gagagtattt gccgaaggca gcaccggctc aaacgaaaat
     3061 ggattttacc ttggggcaaa attttacccg gcacgcggat ggacaataga tgcatatttt
     3121 gatgtctgga aaaatccttg gctgaggttt aatgttgcat caccttctgt ggggcgcgaa
     3181 tatctcgcca aaatacagta ttctatcaag cgcaagtttg atatttatat acaatacaag
//
LOCUS       DAOQQY010000301         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_14994_length_3239_cov_7.003769, whole genome shotgun sequence.
ACCESSION   DAOQQY010000301 DAOQQY010000000
VERSION     DAOQQY010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14994_length_3239_cov_7.003769"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /locus_tag="PLE23_11510"
     CDS             <1..1641
                     /locus_tag="PLE23_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HMX83936.1"
                     /translation="EKTGKPMYRELDLIDVWFDSGSMPYAQWHYPFENVEKFKANFPA
                     DFIAEGVDQTRGWFFTLHAIATMAFDSIAFKNVVSNGLVQDKFGRKMSKRLGNTVEPF
                     ETIAKYGADATRWYMISNAQPWDNLKFDIEGIDEVRRKFFGTMYNTYSFFALYANIDG
                     FKYSEARVPVSKRPEIDRWIISKLNSLIVEVFDFYADYEPTNAARAIQDFVDSHLSNW
                     YVRLCRRRFWKGEYTEDKITAYQTLYECMNTIAKLMAPVAPFFADWLYLNLNTVTESE
                     NYESVHLATFPEEESSAIDPTLEERMDYAQRISSLILSIRKKEKIRVRQPLLKALLPV
                     LDDRFQKQVEAVQDLIMAEVNVKSFEFVTNTAGVISKTIKANFKTLGKRLGKDMKAAA
                     DMISQFDQETIRQIEQQKYYVLNIGDTSYTLEQEDFEIGFNDIPGWQVASDRDLTVAL
                     DITLNDDLIAEGTARDLINRIQNIRKTSDLNVTDRIIIKLESNDAVNVAIDKFADYIC
                     SETLADSLTTEKEVNGERLELFEDVIIGIEVLPVLSVK"
     gene            complement(1630..2718)
                     /locus_tag="PLE23_11515"
     CDS             complement(1630..2718)
                     /locus_tag="PLE23_11515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HMX83937.1"
                     /translation="MFDVILSFITAFTLTYFAIPSIIKVAEIKNLCDEPGTRTSHTRS
                     IPTLGGIAIFAGVSFSVIFWTPFTDFGELQYILCAFVVIFLIGVKDDIIPLTPTKKFL
                     GQLFAAGVLVFKSKITIPSFFGVFGIYELPYWVTILFTTFVILVIINSFNLIDGINGL
                     SGSIGTIITFMLGTWFMLTDKVELSILAFSLAGSLVAFLKYNYSPAKIFMGDTGSMLV
                     GLISATLVIEFIEFHHVTKTGTFTFKAAPAFAFGLLILPLFDTLRVFAVRIMKGKSPF
                     HPDRNHIHHMLIDIGLTHMQATGVLILMNLLFVIIVATLQDLGNLRAIAIILTLAWIL
                     TSYLFFVYRRKMKQSTNDESLQKEHLLH"
     gene            complement(2847..>3239)
                     /locus_tag="PLE23_11520"
     CDS             complement(2847..>3239)
                     /locus_tag="PLE23_11520"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMX83938.1"
                     /translation="IIAGRIIREQIAPEFRNGNYYQGIDKAVDAIIFYTGSEFTAEPE
                     ADHSDTILLFILCFFVLLLLFAYFTRNARPVNRQIIRGDGHTWPGGTWGGWGGGSSGG
                     GWGGGSSGGGFGGFGGGSFGGGGASGSW"
BASE COUNT         1066 a          657 c          675 g          841 t
ORIGIN      
        1 gaaaaaaccg gaaagccgat gtaccgcgaa cttgacctca ttgacgtctg gtttgatagc
       61 ggttcgatgc cttatgcaca atggcattat ccatttgaga atgttgagaa gtttaaggca
      121 aattttccgg cagatttcat agcagaaggc gttgaccaga cacgcgggtg gttttttaca
      181 cttcacgcta tagccactat ggcatttgac agcatagctt ttaaaaatgt agtatccaat
      241 ggtcttgtac aagataaatt tggtagaaaa atgtcgaaac gactgggcaa tactgtcgaa
      301 ccgtttgaga ccatagccaa atatggcgca gatgctaccc gctggtatat gatatcaaat
      361 gctcagcctt gggacaatct caagttcgac attgaaggga tagatgaggt aaggaggaaa
      421 ttttttggta caatgtacaa tacctattcc ttttttgcac tgtatgccaa tattgacggc
      481 ttcaagtatt cggaagctag agttccggta tcaaaaagac cggaaataga cagatggata
      541 atctcaaaac tcaactcgct catagtagaa gtgtttgatt tttatgccga ctatgagcca
      601 accaatgcag cacgggctat acaagatttt gtggacagcc acttgagcaa ctggtatgtt
      661 cgcctatgca ggaggagatt ctggaagggg gaatacaccg aagacaagat aacggcatac
      721 cagacactgt acgagtgcat gaataccatt gccaaactga tggcaccggt agctccattt
      781 tttgccgatt ggttatacct caatctcaat accgttaccg aaagtgaaaa ttatgaatct
      841 gtgcaccttg ccacattccc cgaagaagaa agcagtgcta tagaccctac actcgaagaa
      901 cggatggatt atgcacaacg tatatcatcc ttgattttgt ccataaggaa aaaagaaaaa
      961 atacgagtaa ggcaaccatt gctcaaggca ttgctaccag tacttgatga tcggttccag
     1021 aagcaggtag aagcagtgca ggatctgata atggctgagg taaatgtaaa gtcattcgaa
     1081 tttgtaacga atactgccgg cgttatcagc aagaccatca aagctaactt caagacattg
     1141 ggcaaaaggc ttggcaaaga tatgaaggct gctgctgaca tgatttcgca gtttgatcag
     1201 gagacaattc gtcagatcga acaacaaaaa tactacgtct tgaatattgg tgatacaagc
     1261 tatacgctcg aacaggaaga tttcgagatt gggtttaatg atataccagg ctggcaagta
     1321 gccagtgatc gcgacctgac tgtagcgctt gacataacac ttaatgacga tctcatagct
     1381 gaaggcacgg cacgtgacct tatcaatcgt attcagaaca ttcgtaagac cagcgatttg
     1441 aacgttactg accgcattat tatcaagctt gagtcaaatg atgcagtaaa tgtggcaatt
     1501 gataaatttg ctgattatat atgtagtgaa accctggctg acagccttac aactgaaaaa
     1561 gaggtaaatg gtgaaagact ggagttgttc gaagatgtaa taatcggtat tgaagtattg
     1621 ccggtattat cagtgaagta aatgttcttt ttgtagggat tcgtcattcg tggattgttt
     1681 catttttctt ctatacacaa agaagaggta tgatgtcaat atccatgcga gtgtgagtat
     1741 aatagctatg gctctaaggt tgccaaggtc ttgtaatgta gctacgatga tcacgaataa
     1801 taaattcatt aggatcaata cacccgttgc ctgcatgtga gtaagcccaa tgtctatcaa
     1861 catgtgatga atgtggttgc ggtctggatg aaatggtgat ttgcccttca taattctcac
     1921 agcaaacaca cgcaaggtat cgaacaaagg taatataagt agcccaaatg caaaggctgg
     1981 cgctgctttg aaagtaaacg tacctgtttt ggttacatga tggaattcaa taaattcaat
     2041 caccagtgta gcagatatca acccgaccaa catagaacca gtatcaccca taaatatttt
     2101 tgccggcgaa taattatact tcagaaaggc caccagcgaa ccggcaagac taaatgcgag
     2161 aattgacaat tcaactttgt cggtcagcat aaaccatgta cctaacatga atgtgatgat
     2221 cgtgcctatg cttccggaca agccattaat accatctatg aggttaaaag aattgatgat
     2281 gacaaggatg acgaaagtag taaacaaaat agtcacccaa taaggtagct cataaatacc
     2341 aaaaacacca aaaaacgatg gaatggttat ttttgattta aaaaccagta ctccggctgc
     2401 aaaaagctgc cctaaaaatt tcttggttgg cgttagagga ataatgtcat ccttgacacc
     2461 tatgaggaat atgacaacaa aagcacacaa tatgtactgg agttcgccaa aatcagtaaa
     2521 tggtgtccaa aaaataacgg aaaaagatac tccggcaaat atagcaatac caccaagtgt
     2581 gggaatgctt ctggtgtgag aagtgcgtgt accgggctcg tcacacagat tctttatttc
     2641 ggctactttg atgattgacg gaatcgcaaa gtatgtcaga gtaaaggccg taataaaact
     2701 taatattacg tcaaacatat ttcaagaatt tctggcacaa agttacaatt ttaaagtatt
     2761 gaaagtatcg ttctttccta acaattcaca aacaatcatt cattatgact tgtaaaacaa
     2821 tataaggtaa caaaaataaa attctgttac caactgccac tggcaccacc accaccaaaa
     2881 ctaccaccac caaagccacc aaaacccccg cccgaagagc ctccacccca gccgccgccg
     2941 gaagagcctc caccccagcc accccatgta cctcctggcc acgtatgccc gtcacctctt
     3001 atgatttgtc tgtttaccgg ccgggcattt ctggtaaaat atgcaaataa caataacaga
     3061 acaaaaaagc aaagtataaa taataggatt gtatcagaat gatcagcttc cggttctgca
     3121 gtgaattctg acccagtgta aaaaataatc gcatcaactg ccttatctat accttggtaa
     3181 tagttaccat tacgaaactc aggagctatc tgttcacgga tgattctacc tgctatgat
//
LOCUS       DAOQQY010000302         3231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_15057_length_3231_cov_5.750630, whole genome shotgun sequence.
ACCESSION   DAOQQY010000302 DAOQQY010000000
VERSION     DAOQQY010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15057_length_3231_cov_5.750630"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PLE23_11525"
     CDS             <1..351
                     /locus_tag="PLE23_11525"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMX83939.1"
                     /translation="VFTRFNEMPSVEGQPAMSEADGNLVMHVMLPISAETSLMASDTG
                     SAWSSNFKQGNNCSLSINTTSKDEADHLFNGLSAGGQITMPLEQTFWGAYFGMFTDKF
                     GVHWMVNYDAPQDK"
     gene            complement(417..884)
                     /locus_tag="PLE23_11530"
     CDS             complement(417..884)
                     /locus_tag="PLE23_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMX83940.1"
                     /translation="MQANGSTVAEILANIPDERKGAFTKLHETILDNLPEGFEPGISY
                     GGLGYVVPHSVYPAGYHCKPSEPLPFAGIASQKNSINFYHMGIYADARLYDWFVSEYP
                     KYSKQKLDMGKSCIRFKKPDDIPFELIGELMQKMTSKDWIALYEENYLKKSKK"
     gene            complement(1006..1839)
                     /locus_tag="PLE23_11535"
     CDS             complement(1006..1839)
                     /locus_tag="PLE23_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HMX83941.1"
                     /translation="MKNNSEIKEMVKQKYSEIALQDMAINASSCCGSGGCSTEVYNIM
                     TDEYSHLEGYNPDADLKLGCGLPTEFAKIKPGDTVVDLGSGAGNDCFVARHETGDTGK
                     VIGIDFTEAMIDKARANAEKLQFNNVEFRLGDIEQMPVTSGIADVVVSNCVMNLVPDK
                     PKAFGEIFRILKPGGHFSISDIVLDGELPEKLRKAAEMYAGCVSGAIQQSDYLQILKD
                     AGFSNISVQKEKPIIVPNDILKNYLSEDEILAYHNSNTSIRSITVYGEKKQTCCGPEC
                     C"
     gene            complement(1878..2204)
                     /locus_tag="PLE23_11540"
     CDS             complement(1878..2204)
                     /locus_tag="PLE23_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003002386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMX83942.1"
                     /translation="MGITKTELFTEEQNRMAQIAKALGHPARIAIIEHILKVNQCICG
                     DLVGELPLAQPTISQHLKELKLAGLIKGNIEGNSVCYYIDEQTFILLREYFFNIVNKV
                     SNNNCC"
     gene            2621..>3231
                     /locus_tag="PLE23_11545"
     CDS             2621..>3231
                     /locus_tag="PLE23_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83943.1"
                     /translation="MKALKLLIHSNIYISLAAVALTLATQIQLDMELQWHPYLFLIFF
                     ATLFEYNLHRLITILTNRDALKSEKHSWVSGNLNLFYLLMLFSVLGFLIAVFNAKLRV
                     LLTLAPIAGLTLFYSTPISKGVKGLFRLRQIPFLKIFLIAFVWSSVTILLPVMYSDIH
                     YNAWHIILMITERFLFIFAITIPFDIRDMEVDRLSGLQTIPLQ"
BASE COUNT          910 a          665 c          612 g         1044 t
ORIGIN      
        1 gtattcaccc ggttcaacga aatgccttct gtagaaggcc agcctgctat gagtgaggca
       61 gacggcaatc tggtcatgca cgtgatgttg ccaataagtg ccgaaaccag cctgatggca
      121 agcgataccg gaagtgcctg gtcgtctaac ttcaagcaag gcaacaattg ttcgctctct
      181 attaatacta catccaaaga tgaggcggac catctgttca acggattatc ggccggcgga
      241 caaataacca tgccattgga gcaaactttc tggggagcat atttcggaat gtttacagac
      301 aagttcggtg tacactggat ggtaaactat gatgcgccac aagataaata aatacggaag
      361 aagagaaaaa ctgaacaggt aacattcaga ctcgtttgtc acctgttcag atcttattat
      421 ttttttgact tcttcaaata attctcttcg tataaggcta tccagtcttt ggaagtcatt
      481 ttctgcatca gctcaccgat cagctcaaaa ggtatatcat ccggtttttt gaaacggata
      541 caacttttgc ccatatccag cttttgctta ctatacttcg gatattctga tacaaaccaa
      601 tcgtacagtc gagcatcggc atagatgccc atgtggtaaa aattgatgga attcttttga
      661 gatgctatac cggcaaacgg tagtggttcg gatggtttgc agtggtatcc ggccggataa
      721 accgaatgtg gcacaacata accaagccca ccgtaactga tgcccggctc aaaaccttca
      781 ggcagattgt ccaatatggt ttcgtgcaat ttcgtaaatg ctccttttct ttcgtcagga
      841 atgttagcca gtatttcggc tactgttgat ccattggctt gcataaatca aatgttttat
      901 tttctaaacg tgaatctagc cgatttcatt cgactgggtt taaaaaagat tgaaacaaaa
      961 aatattcaaa attttttcaa agtgaatcac agcgaataaa aatgactaac aacattccgg
     1021 accacagcaa gtttgcttct tttcaccata cacagtgatg ctacgtatgc ttgtgttgct
     1081 gttgtggtag gctaatatct cgtcttcact tagataattc ttcagtatat cattgggcac
     1141 aatgatgggt ttttcttttt gtacagaaat atttgaaaag cctgcatcct ttagtatttg
     1201 tagatagtct gactgttgga tcgcccctga aacacaacct gcatacatct cagcagcctt
     1261 gcggagtttt tctggtagtt cgccatccag tacgatatca gaaatactga aatgtccgcc
     1321 cggtttgagt attcggaaaa tttcaccaaa ggccttaggc ttgtcgggta cgaggttcat
     1381 cacacaatta cttaccacta catcggcaat acctgaggtg acaggcattt gctcaatgtc
     1441 tcctaatctg aactcaacgt tgttgaactg cagtttttcg gcattggccc ttgctttgtc
     1501 tatcatagct tctgtaaaat ctatgcctat taccttgcct gtatcaccgg tttcatggcg
     1561 ggccacaaaa cagtcgttgc ctgcaccgct gcccagatca actacggtat cgcccggctt
     1621 aattttagca aattcggtcg ggagtccaca cccaagcttc aggtcagcat ccggattgta
     1681 tccttcgagg tgcgagtatt cgtccgtcat gatgttatag acctcagtac tgcaccctcc
     1741 actaccacag caagaagaag cattgattgc catatcctga agagcaattt cgctgtattt
     1801 ttgcttaacc atttctttga tttctgaatt attcttcatg ttttttttgg ttaaaaaatt
     1861 taaataaaaa aatatgctta acaacaattg ttgttggaaa ctttattgac gatgttaaaa
     1921 aaatattctc gaagcaaaat gaaagtttgt tcgtctatat aataacaaac agaatttcct
     1981 tcaatattgc ctttgataag cccggccagc tttagttctt ttaaatgttg tgaaatagtg
     2041 ggttgcgcca gcggcaattc accgacaaga tcaccacaaa tgcattggtt gaccttcagg
     2101 atgtgttcga tgatggctat ccttgccggg tgccccaatg cttttgctat ctgcgccatc
     2161 ctgttttgtt cttctgtaaa taattctgtc ttcgttatac ccattgtaat atttttatat
     2221 tgcaatatta cgataatatg aaattgatgt ccaaattatt tttaaacttt aacttaattt
     2281 atttttcaaa aatattggca catctgtatg aatatatgaa attgcgcgca ttcgatgcaa
     2341 tgattactta caatgctgtg ggttggcaag gtgaaatatt gtgtcattac aaagtggttt
     2401 caagatttca tacttatgga aattttactg tggcttgtcc gaaaaattta actaatcttt
     2461 gaaaaataaa ctgattgcat tgacttaatt gatgtaaaaa gctacatttg ttatggtttt
     2521 ctgtatctaa gctcagacag accctggaaa gcagattcga aatgtttagt tgaaattttt
     2581 ttggtaaaaa tcgtttctct cattcagtca aaaaggatcg ttgaaagcat taaaactatt
     2641 aatacactcc aatatctata tttcacttgc tgcggtagcg cttaccttgg ctacacaaat
     2701 acagcttgat atggagcttc agtggcatcc atatttattt cttatatttt ttgcaacact
     2761 gttcgaatat aacctccaca gactcatcac aatactcacc aaccgtgatg cgctgaaatc
     2821 ggaaaaacac agctgggtgt ccggcaatct gaacttgttt tatctgctga tgttgttctc
     2881 tgtacttggt tttttgattg ctgtattcaa tgccaagcta cgtgtattgc ttacacttgc
     2941 tccaatagcc gggcttacgt tgttttactc cacacccatt tccaaaggtg taaaaggcct
     3001 attcaggttg agacagatac catttctaaa aatcttcctc atcgcttttg tatggagtag
     3061 tgtcacgatc ttgcttcctg ttatgtactc agatatacac tataatgctt ggcacatcat
     3121 actgatgata accgaaagat ttttgtttat ttttgctatt acgattccat ttgatatcag
     3181 agatatggaa gttgaccgac tttccggact acagacaata ccattacaaa t
//
LOCUS       DAOQQY010000303         3169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_15568_length_3169_cov_4.930315, whole genome shotgun sequence.
ACCESSION   DAOQQY010000303 DAOQQY010000000
VERSION     DAOQQY010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15568_length_3169_cov_4.930315"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLE23_11550"
     CDS             <1..408
                     /locus_tag="PLE23_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMX83944.1"
                     /translation="RAAAQNIVPTTTGAAEAVAKVWPSVQGKLFGKALRVPVITGSLT
                     DLTCILSRKVTEAEINEAFRKAAEGSMKGIVQYNEVPLVSSDIIGNKHSSIFDAPLTK
                     VTGNLVSVVAWYDNEAGYSARLSDLVMIIARRF"
     gene            complement(463..1254)
                     /gene="mazG"
                     /locus_tag="PLE23_11555"
     CDS             complement(463..1254)
                     /gene="mazG"
                     /locus_tag="PLE23_11555"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HMX83945.1"
                     /translation="MHSESTQAFERLLGIMDDLREKCPWDRKQTMQSLRNLTIEEVFE
                     LADAISEGDVNGVKEELGDVMLHLVFYGKIAAENYDFTLAESIHAVCEKLISRHPHIY
                     ADVVVQGEEDVKRNWEKLKLKEGKKSLLSGVPKSLPAMIKAYRMQEKTAQVGFEWENP
                     EQVWAKVQEELTELRTEIFSNEKNQDKIEEELGDVFFALINYARFIGVDPENALQKVN
                     SKFKSRFEYIEDHAPRPLSEMTLEEMDLLWNQAKGKNHNSEALKY"
     gene            complement(1260..1850)
                     /gene="ribA"
                     /locus_tag="PLE23_11560"
     CDS             complement(1260..1850)
                     /gene="ribA"
                     /locus_tag="PLE23_11560"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HMX83946.1"
                     /translation="MKRQVETKVPTTWGNYKVIAYADSEDEQNPTMAFVHEDINIHEP
                     VMVRLHSECLTGDVFHSLKCDCGPQLEKSMQMIRENKGVLIYLRQEGRNIGLINKLKA
                     YQLQDTGMDTFEANVALGFDYDQRKYDLAVEILEDLGISRVRLITNNPEKIEAIDNSA
                     TIELVERVKIDIPHNRFNTKYLQAKKETMGHLINTK"
     gene            complement(1907..>3169)
                     /locus_tag="PLE23_11565"
     CDS             complement(1907..>3169)
                     /locus_tag="PLE23_11565"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83947.1"
                     /translation="NRYKNYANADGGFNYWPGGNYSNNWATIYAGHFMVEAKNAGYNV
                     PNNLLESWIKSQRKMASVWSAKQYESGLYEANSTLVQAYRLYVLALGRSADKGAMNRL
                     REVARLDRTSIALLAAAYALDGKKEIARTLINMKTGSIPTYDYAGYTYGSELRDESIL
                     LETYQLCGMSEQADKLAVDISKYLNKHGTRYSTQTLAFSLSALGKYIGRNTKAGRELH
                     FAYSLDGKKEITVKTKDAIMLIELNTDEAATGKLKLRNLGSAPLYSSVTLTGKPAMGK
                     ELEDARNLEMTMRFTDLNGQQLDPQKLTRGKEFIVSIQVRNPGSLSWDYTNLALTQVF
                     PSGWEINNPRMSDVNFARSSGGIEYQDFRDDRVLSYFGLGRNREMNVQIQLTAAYPGR
                     YYLPGTQCEAMYDGEVYSHKKGMWVTVL"
BASE COUNT          805 a          711 c          652 g         1001 t
ORIGIN      
        1 agagctgccg cacagaacat cgtacctact accaccggtg cagcagaagc ggtggctaaa
       61 gtgtggccaa gcgtgcaggg aaaactattc ggcaaagctc tgcgtgtacc tgtcatcacg
      121 ggttcactca ccgatctcac ttgtatcctt tccagaaaag tcacagaagc cgaaataaac
      181 gaagcatttc gcaaggcagc cgaaggctct atgaagggta ttgtgcaata taatgaagtg
      241 cctttagtga gtagtgatat aataggcaat aaacattcaa gcatattcga tgctccactg
      301 accaaagtca ccggcaatct tgtatcagtt gtagcgtggt acgataatga agcaggctat
      361 tcggcacgcc tgtctgatct ggttatgatt attgcaagaa ggttttaaca caggaaatat
      421 cagccaaatc aggctaaaag ctactttgaa gcaagatgca gatcagtatt tcaacgcttc
      481 agagttgtgg ttctttccct ttgcctgatt ccatagtaga tccatttctt caagagtcat
      541 ttcactgaga ggccttggtg catgatcttc aatatattcg aaccgtgatt tgaatttgct
      601 atttaccttt tgtaaagcat tttcggggtc aacacctatg aaacgtgcat aatttattag
      661 tgcaaagaat acatcaccta attcttcttc tattttgtcc tgattctttt cattgctgaa
      721 tatttctgta cgaagttcgg taagttcttc ttgtactttt gcccatactt gttcaggatt
      781 ttcccattcg aagcccacct gtgctgtttt ctcttgcata cgatatgctt taatcattgc
      841 cggaagtgat tttggcacac cagagagaag cgatttctta ccttccttta gtttgagttt
      901 ttcccagttg cgttttacgt cttcctcccc ttgtaccaca acatcagcat agatgtgcgg
      961 gtgacgagaa atgagttttt cgcatactgc atggatggat tcggccaagg taaaatcata
     1021 gttttcggcg gcaattttgc cataaaatac aaggtggagc atcacgtcgc ccaattcttc
     1081 ttttacacca ttgacatcgc cttcagagat ggcatctgcc agttcgaaca cctcttctat
     1141 ggtaagattg cggagagatt gcatggtctg ctttctatcc caagggcatt tttctctaag
     1201 gtcatccatt atgccaagga gacgctcgaa tgcttgtgtg gattcgctat gcatcgatac
     1261 tattttgtat tgataaggtg gcccattgtt tctttttttg cttggagata ctttgtatta
     1321 aacctgttgt gtggaatgtc aatttttaca cgttcgacga gttcgatggt agctgagtta
     1381 tcgattgcct caatcttttc aggattattc gtaatcagtc gaacccggct tataccgaga
     1441 tcttcgagga tttcgacagc aagatcatat tttcgctggt cgtaatcaaa accaagtgct
     1501 acatttgctt caaatgtatc cattcctgta tcctgtagct ggtaagcttt aagcttgttg
     1561 atgagcccaa tatttcgacc ctcctggcgc aagtaaataa gtacaccctt gttttcacgg
     1621 atcatttgca tcgatttttc gagctgaggc ccgcagtcac atttaagtga atgaaaaacg
     1681 tcaccggtaa ggcattctga atgaagccga accattacag gctcgtggat gtttatatct
     1741 tcatgtacaa atgccattgt cggattttgt tcatcttccg agtctgcata agcgatcacc
     1801 ttataattgc cccaggtagt aggcactttt gtttctactt gtcttttcac tattttcttt
     1861 caatattcta acagaacaac aaaacttgct tgagggttca aaattactac aaaactgtca
     1921 cccacatacc ttttttatgc gaataaactt ccccatcgta catggcctcg cattgtgtac
     1981 ccggaagata atacctgcca ggatatgctg ctgtgagttg aatctgaacg ttcatctctc
     2041 tgtttctgcc caggccgaaa taactcaaga ccctgtcatc acgaaaatcc tgatactcaa
     2101 taccacctga agagcgtgca aaattcacat cactcattct cggattgttg atttcccatc
     2161 ccgatgggaa cacctgagta agggccagat tggtataatc ccagcttagc gaacccgggt
     2221 tgcgtacttg gatactgact ataaattcct ttcctctggt aagtttttgc ggatcgagtt
     2281 gctggccatt taaatcagta aagcgcatcg tcatttcaag attgcgagcg tcttcgagtt
     2341 ccttacccat tgctggtttg cctgtaagtg ttacacttga ataaagtgga gcagaaccaa
     2401 ggtttcttaa tttgagtttt cctgttgcag cctcatctgt attcagttcg atgagcatga
     2461 tagcgtcctt tgtttttaca gtaatttcct tttttccatc gaggctatat gcaaaatgta
     2521 attccctgcc ggccttagta tttctgccta tatatttgcc caaagcactc aaagagaaag
     2581 cgagtgtttg ggtactgtat cttgtgccgt gtttgttcag gtatttactg atgtcaacag
     2641 cgagtttgtc tgcctgttcg gacattccgc agagctggta agtttcgagc aagatagact
     2701 catccctcaa ttctgaacca tacgtatagc cggcgtaatc gtaggtaggg attgatcctg
     2761 tcttcatgtt aataagtgtt cgtgctattt cctttttacc atcaagcgcg taagctgcag
     2821 ctaacaatgc aatactggtg cggtcaagtc tggctacttc ccgaagtcgg ttcatcgcac
     2881 ctttatcggc cgagcgacca agcgccagaa catataatct gtatgcctgc acaagcgtag
     2941 aatttgcctc gtacagtccg gattcgtatt gctttgcact ccaaacagaa gccatttttc
     3001 tttgagactt gatccacgat tcgagcaggt tgtttggaac attgtatccg gcattttttg
     3061 cctcaaccat gaaatgcccg gcatagatgg tagcccaatt attggaataa ttgccacccg
     3121 gccaataatt aaaacctcca tcagcgtttg catagttctt ataccgatt
//
LOCUS       DAOQQY010000304         3166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_15583_length_3166_cov_5.667631, whole genome shotgun sequence.
ACCESSION   DAOQQY010000304 DAOQQY010000000
VERSION     DAOQQY010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15583_length_3166_cov_5.667631"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="PLE23_11570"
     CDS             <1..405
                     /locus_tag="PLE23_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HMX83948.1"
                     /translation="VRMDQQAANAKEVAAFLSTHPKVERVYYIGHLDSNDAEFEVFNK
                     QCVSPGAMVSFDIKGGEAEAFAFLNNLKLIKLAVSLGSTESLAEHPDTMTHAGVDPGH
                     KLEMNITGKMVRLSIGVENFNDIIWDIKQALS"
     gene            complement(474..>3166)
                     /locus_tag="PLE23_11575"
     CDS             complement(474..>3166)
                     /locus_tag="PLE23_11575"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX83949.1"
                     /translation="PVASATVTSPITCDSVSVTLTGISSLNLPTITYSWSGPGGFSAN
                     TKYTKATVTGQYTVTVTNTQNGCTDSADIDVLQNTTAPNVTAKGDTTDCISGQATLTG
                     GSSTPNAVYQWFNATGVPLCPTANCDVAGAGIFTLVVTDPVNGCTSSQSVESVKDQDT
                     PDITLDKDDDLDCNITTVNLTATSSVLGLTYAWTGPGVPGGSVTNISTSTPGIFTVKV
                     TNPKNQCTNSASIQVDQDIVKPVISAKTDTINCYNNKTATINGTSDVTNNITVSWVDP
                     SSNAVSSNLNFTTQTAQTYTLIVTNNSNGCSNTLNLKVPENTTVPNISAQGDVINCYE
                     PIAECVGNSSTNNVKYAWSGPGNYSANTSNADLITTAGDYTLVVTDIKNGCTSTMTVI
                     VVQDNANPDVNATGGTLTCTNNSQIQLTASTSATPVTWVWSGPNNFYSTQQNPTVGDA
                     GTYTVVVTDTGNGCTQTTTANVTSDENAPDLSVNDAVIDCINTSQTLNAVSNTPGVTY
                     AWSGPGINTTTPSVTVTQAGNYVCVVTAPNGCTTSKTSTVTLNSDLPNALAQVSGELN
                     CTTKLVNVTTTGSSIGNQFSYSWTGPGGFTGNQTNFSVATPGVYSLVITNTSNGCTKT
                     TDIEVPINNEMPTGLTTEQGNPKCFGNKDGFIIVNGVTGGTAPFTYSINGGTFNTASE
                     FQFLGDGTYKISIQDAAGCEYDTLLTIKQPALFTVEAGKDTIIGWGNSITLGIDNISD
                     LGRLKTLEWTPALDSFCANCPNPTVQLFNAQLFTVTATDTSGCVATDKKLVFVKKERL
                     VYIPNTFSPNGDGLNDFFGIQTGPGVEEIKKFEIFDRWGNKIFEKENFLPGPNSDTVN
                     GWNGQYRGKVVNSGVFVYWAMIKFLDGEEILYKGDVTVQR"
BASE COUNT          858 a          642 c          710 g          956 t
ORIGIN      
        1 gtgagaatgg atcaacaggc tgccaatgca aaggaagtag cagcatttct aagtacacat
       61 cctaaagtag agcgcgttta ttatataggt catctggaca gcaacgatgc tgagtttgaa
      121 gtatttaaca agcaatgtgt ctcaccgggt gcaatggttt cgtttgatat taagggtggc
      181 gaagcagaag catttgcttt tctgaataat ctaaaactca taaaacttgc agtaagtctt
      241 ggcagtacag agtcacttgc agaacatcct gacaccatga cacatgccgg ggttgacccg
      301 gggcataagc ttgaaatgaa cataacagga aaaatggttc gattgtcaat tggggtagaa
      361 aacttcaatg acatcatctg ggacattaag caggccttat cctgatcacc acataaagga
      421 aaagcccgca agaattactt acgggctttt cttttgaata ttgtaacagg attttatctt
      481 tggacagtta catcaccttt atacaggatt tcttcaccat ccaggaattt aatcattgcc
      541 cagtaaacaa atacgccact gtttaccacc tttccgcgat attgaccatt ccatccgttg
      601 actgtatcac tgttagggcc aggtaggaag ttttcctttt caaatatctt attaccccaa
      661 cggtcaaaga tctcgaattt cttgatctct tcaactccgg gtcctgtctg gatgccgaag
      721 aagtcattga ggccatcacc gtttggtgaa aaagtattag ggatgtaaac cagcctttct
      781 tttttaacaa atacaagttt tttatcagta gccacacagc cagaagtatc agtagccgta
      841 acagtaaaca actgggcatt aaataactgt actgttgggt tcggacaatt tgcacagaaa
      901 gaatccagag ccggtgtcca ttcaagtgtt ttgagcctgc ctaggtcact gatattgtca
      961 atacccaaag tgattgaatt gccccatcca atgatggtat ccttgcccgc ttcaactgtg
     1021 aacaatgcag gttgtttgat agtaagcaat gtgtcgtatt cgcagcccgc tgcatcttga
     1081 atagaaatct tgtaagtacc gtcaccaagg aattgaaact ccgaagcagt attaaatgtg
     1141 ccaccattga tagaataagt aaatggagca gtaccgccgg tcacaccgtt tacaatgata
     1201 aatccgtctt tatttccaaa acatttagga ttcccctgct cggtagtaag acctgtaggc
     1261 atttcgttat tgattggaac ttctatgtca gttgtttttg tacagccatt actggtattg
     1321 gtgatgacaa gtgagtaaac gcccggtgtt gctacagaga aatttgtttg gttgccggta
     1381 aaacctcccg gaccggtcca gctgtagcta aactggtttc cgatgcttga gcctgttgtt
     1441 gtaacattga caagttttgt tgtacagttc agttcgccac ttacctgagc gagagcattc
     1501 ggcaaatcag aattaagtgt tacggtagaa gtctttgatg tagtacagcc gtttggtgca
     1561 gttactacac atacataatt acctgcttgt gtaaccgtaa cagacggcgt ggtagtatta
     1621 atgccaggcc cactccaagc gtacgtgaca cctggtgtat ttgacactgc attaagagtt
     1681 tgagaagtat ttatacagtc tattacagca tcgtttactg acaagtccgg tgcattctca
     1741 tcacttgtta catttgcagt agttgtctgg gtgcaaccat ttccggtatc cgtaactact
     1801 acagtgtaag tacctgcatc acctactgta ggattttgct gggtagagta gaagttgtta
     1861 ggaccactcc aaacccacgt gacaggcgta gctgaagtag aagctgtaag ttgtatctgc
     1921 gaattatttg tacaagtaag ggtgccacct gttgcattaa catcagggtt ggcattatcc
     1981 tgtactacaa ttacagtcat agtacttgta caaccattct ttatatcagt cacaacaagt
     2041 gtataatcac ctgcagtggt aatgaggtct gcatttgaag tattggcaga atagttaccc
     2101 gggccactcc atgcatattt tacattattg gttgatgagt tgccaacaca ttcagcgatt
     2161 ggttcatagc aattgattac atcaccttgt gccgaaatgt ttggaacggt agtgttttca
     2221 ggtactttaa gattaagagt gtttgaacag ccattactgt tgttggtcac tatcagcgta
     2281 tatgtctgtg cagtttgtgt tgtaaaatta aggttactgc ttactgcatt tgaagatggg
     2341 tcaacccatg atacagtaat attattagta acatccgaag tgccgtttat ggtagctgtt
     2401 ttgttattgt aacaatttat tgtgtcggtt ttagcagaaa tgacaggttt cactatatcc
     2461 tgatcgacct gaatgcttgc cgagttggta cattggtttt tcggattggt caccttaacg
     2521 gtgaatatgc cgggagtgct agtagagata tttgtaactg acccaccagg tacaccagga
     2581 ccagtccatg cgtatgtgag ccccagtaca cttgaagtag ctgtcagatt tacggttgtt
     2641 atgttacagt ccagatcatc atctttatct aaagtaatgt caggtgtatc ctggtctttg
     2701 acagattcga cactttgaga gctagtacaa ccattgacag ggtctgtgac gacaagtgta
     2761 aaaatgccag caccggctac atcacaattg gcagtagggc agagcggcac acctgtggca
     2821 ttgaaccact gatatactgc atttggtgta ctggatccac ctgttaaggt cgcctgacca
     2881 gagatacagt cagtcgtgtc acctttagct gttacgttcg gtgcagttgt attttgtagt
     2941 acatcaatat ccgcactgtc tgtacatccg ttttgggtat tggtcacggt cactgtatat
     3001 tggcctgtaa cagtggcctt tgtgtatttt gtattggcag aaaatccacc cggaccgctc
     3061 catgaatagg tgatggtcgg cagattaaga ctcgatatgc ctgtaagtgt tactgagacg
     3121 ctatcacagg tgatcgggct tgtcactgtg gctgatgcta ctggtt
//
LOCUS       DAOQQY010000305         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_15638_length_3158_cov_7.202063, whole genome shotgun sequence.
ACCESSION   DAOQQY010000305 DAOQQY010000000
VERSION     DAOQQY010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15638_length_3158_cov_7.202063"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /locus_tag="PLE23_11580"
     CDS             <1..1504
                     /locus_tag="PLE23_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX83950.1"
                     /translation="LIAAAELSHRYIADRKLPDKAIDLIDEAAAKLRLELDSVPDEVD
                     ELDRKIRQLEIEREAIKKEKDQEKLGKLNEQIANLKEDYNVIQTRWKNEKEILDSIQA
                     SKTAIEEYENQAQKAVRDSNFELVATLRYGKIKEEEKKLAELEEKLKELSPEKRLTNP
                     EVTADDIAEVVSKWTGIPVSKMLQSEKEKLLHLEDEIGKRLIGQKEAVQAVSDAIRRN
                     RAGLSDMKRPIGSFIFLGPTGVGKTELAKALAEVLFDDESAITRIDMSEFQESHAVSR
                     LIGAPPGYVGYDEGGQLTEAVRRRPYSIVLLDEIEKAHPDTFNILLQVLDDGRLTDNK
                     GRVADFKNTIIIMTSNLGSDIILENFEDLEAAGENKRFEIVEATKAEVFDLLKQRLRP
                     EFLNRIDEQIMFQPLSKKEIREILGLLMKGLNKMLDNQGLALELTDATKDYLAALGYE
                     PQYGARPMKRVLQRELINELSKELLSGVYSKGDTIIADTDHKKILFRKKV"
     gene            1741..2739
                     /locus_tag="PLE23_11585"
     CDS             1741..2739
                     /locus_tag="PLE23_11585"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX83951.1"
                     /translation="MKNILTVLIVLLFSANVMFSQSEKKVVVEHFTNSNCSICANRNP
                     GFFNNLKNFPDVIHISFHPSSPYPSCLLSQQANPGNDARTNYYGIYGGTPRLVIQGVV
                     ISANASYSSPDLFAPYINKTAPVEIEIEQYKYGQDSIKAVVTIAATQNNQLSSAKLFV
                     AVAEDTVFYKGTNKETMHFNVFRQAMSDIEGTTVNVPSVAGQSVSYTFTVPTKAFWNM
                     SRMFTIAILQDASTKAVIQAESESPEEDETTATQAPKPLAGINIYPNPVSGFLTVELA
                     GSEESELSVFDSTGKLVRRKTFRQSTSIDTQDMAKGIYMAEIKNMRGKTVKKIVVE"
BASE COUNT         1001 a          639 c          698 g          820 t
ORIGIN      
        1 gctgatagcc gcagctgagc tctcgcaccg ttatattgcc gatcgcaagt tgccggataa
       61 agctatagac ctcattgatg aagccgccgc caagctcagg cttgaactcg acagcgtacc
      121 tgatgaagtg gacgaacttg acaggaagat caggcagctc gagatagaac gcgaagccat
      181 caaaaaagaa aaagaccagg aaaagctcgg taagctcaac gaacagatag ccaacctgaa
      241 agaagactac aacgttatac agacacgctg gaaaaacgaa aaggaaatcc tcgattcgat
      301 tcaggcatcc aagacagcca ttgaagagta cgaaaatcag gcacaaaaag cagtgcgtga
      361 cagcaatttt gaacttgtag caacccttcg ttatgggaaa atcaaagagg aagagaaaaa
      421 attggccgaa ctcgaggaaa agctaaagga actttcacca gagaaacgcc tgaccaatcc
      481 ggaagtaacg gctgatgata tagccgaggt tgtatcaaaa tggacaggta tcccggtgtc
      541 gaaaatgcta cagagcgaga aagaaaaact gttgcacctt gaagacgaaa taggcaagag
      601 gctcatcggt caaaaagaag ccgtacaagc tgtgtctgat gccatacgaa ggaatcgtgc
      661 cggtttgtct gacatgaagc ggcctatcgg ttcgtttatt ttccttggac ctacgggcgt
      721 aggtaaaaca gaacttgcca aggcacttgc agaagtgtta tttgacgatg agagcgccat
      781 cactcgtatt gatatgagcg aatttcagga gtcgcatgct gtttcgcgtc tcataggtgc
      841 acctccgggt tatgttggtt atgacgaagg agggcagttg actgaagctg taagacgaag
      901 accctattct atcgtgctgc ttgatgaaat agaaaaagcg cacccggaca cattcaatat
      961 cttgttgcag gtgctggatg acggaaggct taccgacaac aaaggcagag tagccgattt
     1021 taagaatacc atcatcatca tgacatcaaa cctcggttcg gacatcattc tcgaaaattt
     1081 cgaagacctg gaagccgctg gtgaaaacaa acgattcgag atagtagagg caaccaaagc
     1141 cgaagtattc gatctgctca aacaaagact gcgaccagag ttcctcaaca ggatcgatga
     1201 gcaaattatg ttccagccac tgagcaaaaa ggaaataaga gaaatactcg gattactgat
     1261 gaaaggcttg aacaaaatgc tggataacca agggcttgct ctagagctta cggatgcaac
     1321 caaggattat ctcgccgcac ttggttacga gccgcaatat ggtgcccgac ctatgaaacg
     1381 agtacttcaa cgtgaactta tcaacgaatt gtcaaaagaa ctgttgagtg gagtctatag
     1441 caaaggcgat accatcattg ccgataccga ccacaagaag atattgttca gaaagaaggt
     1501 ataaatgaat tgataataat acaaatgccc cggtctgtat agtgcaggcc ggtttttttc
     1561 tgtttggtaa aatttgccag aaaactcgtg cacccttcat ctcccattgc cggatagaat
     1621 tgattttcct tcagtttgat agtatattgt agtagaaaaa cttgatactg aatgaaaata
     1681 ccggtaattt tgtttttcaa tatatgcgga ttatttaaat ataacaaaca ctttagtctt
     1741 atgaaaaata tccttactgt cctgattgta ttattatttt ctgcaaatgt catgttttcg
     1801 cagtcagaaa agaaagtggt ggtcgaacat ttcaccaact ccaactgcag catttgcgct
     1861 aaccgcaatc cgggattttt caataacctt aagaattttc cagacgttat ccatatttca
     1921 tttcatccaa gttcgcctta tccgagttgt ttactcagcc agcaggcaaa tccgggcaat
     1981 gatgctcgta ccaattatta cggcatttat ggaggtacac ctcgtcttgt catccaaggt
     2041 gtcgttatat cggccaatgc cagctacagt tcgcccgact tgtttgcacc atatatcaac
     2101 aagactgctc ctgttgaaat agaaatagag cagtacaagt acggacaaga ttccattaag
     2161 gcagtagtga ccatcgctgc tacacaaaac aatcagttat cttcagcaaa actgtttgtc
     2221 gcagtggcgg aagacaccgt tttttataaa ggtaccaaca aggagacgat gcacttcaat
     2281 gtgtttaggc aggctatgtc agatattgag ggcaccaccg tgaatgttcc ttccgttgct
     2341 ggccaatcag ttagctacac ctttacggtg ccgacaaaag cattctggaa catgagtcgc
     2401 atgtttacca tcgcaatact tcaggatgca tcaaccaagg ccgtcattca ggcagaatcc
     2461 gaatctcctg aggaggatga gacaactgct acacaagcac caaagccgtt ggctggcatc
     2521 aatatttatc cgaatccggt tagtggtttc cttactgtag agttagccgg tagtgaggag
     2581 tcagaattat cggtttttga ttctacaggc aaacttgtaa gaagaaagac tttccgtcaa
     2641 tcgacaagca tagacacaca agatatggcg aagggcatct atatggctga aataaaaaat
     2701 atgagaggca aaacggtgaa gaagatagtg gtggaataga attgcaataa tattgaatca
     2761 agattctgct ttaggaattt agtcgaaaat gattcgtact aaaaattcca tgaaaaagag
     2821 ataaaagtat agtgaactat ggttttgagc ctttgtattg gatttgctgc aaagagtcta
     2881 atgcgttatc tgggttgaaa agttgttttt gtgtaaaatt ttctttggcc tgatttaaat
     2941 cattgttttg gtttgaacga gtagctgata tttgcagcat gttaatctgg aaaaacatta
     3001 tccgtcagcc tgactgaacg ctgtctttca ttttctttta catggagata ggcatttccc
     3061 gatttattat ctcatcattt tcatttcact cattaaacat tttttatgaa aaccatcata
     3121 gaaccattca ggataaaatc tgtagaaccc atccgttt
//
LOCUS       DAOQQY010000306         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_15770_length_3143_cov_4.321567, whole genome shotgun sequence.
ACCESSION   DAOQQY010000306 DAOQQY010000000
VERSION     DAOQQY010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15770_length_3143_cov_4.321567"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="PLE23_11590"
     CDS             complement(<1..777)
                     /locus_tag="PLE23_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83952.1"
                     /translation="MSRLKLSILSFFLFSTGIINAQVLSNDFLNIGVGARALGMSGAV
                     VASTNDVTSLYWNPASLTGMTSPLSVGAMHAELFAGIAKYDFIGVTKQLNAEKNSAGG
                     IALIRYGIDNIPNTLNLVNPDGSINYDNVYLFSASDYSFILSYAQQIKKGSPFSIGGN
                     LKIINRHVGQFATAWGFGLDAALLYQKNKWTFGATVRDLTTTFNAWSFNFTDKEKATL
                     ESTGNTIPENSTEITKPTIIIGAAKSFNLGKDYTLTPEINA"
     gene            1244..2962
                     /gene="ggt"
                     /locus_tag="PLE23_11595"
     CDS             1244..2962
                     /gene="ggt"
                     /locus_tag="PLE23_11595"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HMX83953.1"
                     /translation="MKSRILLSGFVVALLFLVACSPKSLPKDTYRIQKQVAGNKGIVV
                     CAHPLASAVGADILSQGGNAIDAAVAVQLALAVVYPGAGNIGGGGFLVYREVGGEATT
                     LDFREKAPVAAHRDMYLDKDGNPVMNLSTIGHLAVGVPGTIDGIFEMFEKYSKLKNFS
                     LLIQPAIDLASKGFRITEREADQLNEHRAQFLKVNHVYIPFVKETKWRKGDLLVQTEL
                     ASTLERIKENGRGEFYEGQTAKLIVKCMEQNKGIITEEDLKNYKSVWRQAVKGIYKSY
                     KIITMPPPSSGGILLLQMLKMIEYAQISKLSFHSKEHVHLVTEAERRAYADRAAYFGD
                     SDFTKTNLKHLLDYSYIMSRMKSYCLDQATPSSSIAAGVMPLPHEREETTHISIIDID
                     GNAVSLTTTLNGSYGSMAVVPGGGFLLNNEMDDFSIKPGVPNIYGLVGGEANAIQPGK
                     RMLSSMTPIIVLKDDKLYAIIGSPGGSTIITTVLQIIMNLVDFELNPYDAVQAPRFHS
                     QWLPDKIFLESSCLDYSTRSALKEMGHSIEERSKIGRVEAIVKYKGKYVGVADERGDD
                     AAEAAR"
BASE COUNT          984 a          594 c          662 g          903 t
ORIGIN      
        1 ggcatttatt tcaggtgtca gggtgtaatc ttttccaaga ttaaaacttt ttgctgcacc
       61 tatgatgatg gttggtttgg taatttcagt agaattttca ggaatggtat tcccggtcga
      121 ttcgagagtt gccttttctt tgtcagtaaa attaaaagac caagcgttga atgtagtagt
      181 caggtctctg actgttgcgc cgaatgtcca tttgtttttt tggtataaca gggcggcatc
      241 aaggccaaag ccccaggcag tagcaaactg gcctacatgc cggttaatga tttttagatt
      301 accgcctatt gaaaacggac tgcctttctt tatctgttgt gcataagata gaatgaatga
      361 ataatcggat gctgaaaaca gatacacatt gtcataattg atactgccat caggatttac
      421 cagattcagt gtattgggta tattgtcaat accatagcgg atgagggcaa taccaccggc
      481 tgaatttttc tctgcattca attgtttggt gacacctatg aagtcatatt ttgctattcc
      541 tgcaaaaagt tcggcgtgca tagcacctac agaaagtgga ctggtcatac cggtcaagga
      601 tgccggattc cagtagagtg atgttacatc gttggtggaa gccactactg cacctgacat
      661 accaagtgcc cttgctccta caccaatatt gaggaaatca ttactcaaca cctgcgcatt
      721 gattatgccg gtcgaaaata aaaagaagga aagtatgctg agttttagcc gactcatatt
      781 gtcgcaaata attttgttaa ataaatctta ataaagaaac gctaaagtac aaatcaaatt
      841 gcagaagatg caaaaaaaag tgccatctga acgtacagac ggcacaaaac ataaccatga
      901 aaataactag gcaaacgagt ttatttgcaa aagcaaaaat atcttaacaa attacagctt
      961 tgtaaaaagg cagtatttga ttatattatg tatgtttacg gagataatcc tttaattaac
     1021 gacttgtatg gttaaaacgt aaacgattta tgatcacaaa aaacggtttt gagacccaac
     1081 cttatagaaa aggttaatcg ttcacaggat tataattgtt gttatttgat atgttgaaag
     1141 ccgaagcttt acgtttcatt gtatatttta tataaaacca aataccatac caaactggaa
     1201 agtgtaccgc aataattttt taatttttta attttttttt gatatgaaat ccagaattct
     1261 tctgtccggg tttgtggttg ccttgttatt tctcgtggct tgcagtccta aatctttgcc
     1321 aaaggataca tatcgcattc agaaacaagt agccggaaat aaaggaatcg tggtatgtgc
     1381 acatccgctt gcatcagcag taggtgcaga catccttagt caaggcggca atgccattga
     1441 cgcagccgtt gctgttcagt tggctcttgc tgtagtctat ccgggagcag gtaacattgg
     1501 cggaggcgga tttttggtgt acagagaggt aggaggcgaa gctacaacac ttgattttcg
     1561 tgaaaaagct ccggtagctg cccatcgtga tatgtacctc gacaaagatg gcaatccggt
     1621 aatgaatctt tctacaatcg gtcacttggc ggttggtgtg ccgggtacca ttgacgggat
     1681 attcgaaatg tttgaaaaat attccaagct taaaaatttt tcattgctga tacagcctgc
     1741 tattgatctg gcatccaagg gttttaggat aacagaacgc gaggctgatc aactaaatga
     1801 gcaccgagca cagtttttga aggtaaacca tgtttacata ccctttgtaa aggaaactaa
     1861 atggagaaaa ggcgacttgt tggtacaaac agagcttgcc tctacacttg aacggataaa
     1921 ggaaaacggc agaggagaat tttacgaagg tcaaaccgca aagctgattg taaaatgtat
     1981 ggaacaaaac aaaggaatca taacagagga agacctgaaa aattacaagt ctgtgtggcg
     2041 tcaagctgta aaaggtatat ataaatcata caaaatcatt accatgccgc ctccttcgag
     2101 tggtggtatc ctcctgctcc aaatgcttaa aatgatagaa tatgctcaaa tatcaaagct
     2161 tagctttcac tcgaaagaac atgttcatct ggtgactgaa gcagaaagac gtgcctatgc
     2221 cgaccgggct gcctacttcg gagactccga ttttacaaaa acaaacctaa aacatctcct
     2281 cgattattcc tatataatga gcagaatgaa atcatactgt ttagatcaag caacaccttc
     2341 atcatctatt gctgcaggtg ttatgccttt accacacgag cgtgaagaaa caacacacat
     2401 cagcatcatt gacatagacg gtaatgctgt ttcccttact acaactctca acggttctta
     2461 tggttcgatg gctgtagtgc ctggtggcgg atttttactc aacaacgaaa tggacgattt
     2521 cagcatcaaa cccggcgtac ctaatattta tggtcttgtg ggtggtgaag caaatgccat
     2581 tcaacccgga aaaagaatgc tcagctccat gacacctatt atagtgctca aggatgacaa
     2641 attgtatgcc atcattggtt cgccgggcgg ttctacaatt attacaactg tattgcagat
     2701 cattatgaac ctcgtagatt tcgaattgaa tccgtacgat gccgtacagg caccgaggtt
     2761 ccattcgcaa tggctccctg ataaaatttt tcttgaaagt agttgcctgg attacagtac
     2821 aagatcagca ttgaaggaga tggggcacag tattgaagaa agatcgaaga ttggtagggt
     2881 agaagcgata gtaaaatata aaggcaaata tgtgggtgtg gcagacgaac gcggcgatga
     2941 tgcagccgaa gcagccagat aatgcttcat tcgtccgatt ttttctgcta tgtgtcttaa
     3001 ttttttcaat ctccaaaaga agatgaaact tatttattcc gaataaggtt atctttgtac
     3061 cattaattca caaattcaaa tttcacaaat gaaaaaaatc ttcattgctt tactaagcat
     3121 gtttgtaata gcacagacag gca
//
LOCUS       DAOQQY010000307         3135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_15831_length_3135_cov_5.701299, whole genome shotgun sequence.
ACCESSION   DAOQQY010000307 DAOQQY010000000
VERSION     DAOQQY010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15831_length_3135_cov_5.701299"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLE23_11600"
     CDS             complement(<1..465)
                     /locus_tag="PLE23_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83954.1"
                     /translation="MRILLFFCLLVFSVSAFGQRKKIEIKTDGERYGRSLGLNFYGDT
                     ANRVPLKFLSFKELQAFRNYEFDTIPERCDWRNNSVMIRINQKSKKIPYINSLGDLGE
                     DPEDTGVRISMYTDIFNSCAFVYTNMTLLGYEYSKRIASNVKIFDAAGNLIRE"
     gene            complement(462..986)
                     /locus_tag="PLE23_11605"
     CDS             complement(462..986)
                     /locus_tag="PLE23_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83955.1"
                     /translation="MGINSLPNGLVPADISKKVGIGVAQPQGDCMLTVESGLKVGIYS
                     DYFGSGVGILSKVYNPQTKAFSVSLANNGSSCETFRVYGNGKVECKEVTIRNYGWCDY
                     VFEPDYKLMSLAELKSYVMTNKHLPEVPTEADINANGMDVFETQKLLLKKVEELTLYM
                     ISLDEQITALKKKE"
     gene            1376..2032
                     /locus_tag="PLE23_11610"
     CDS             1376..2032
                     /locus_tag="PLE23_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83956.1"
                     /translation="MKKYSLLVSLLLSGIIIFGFQNCKKEHLPDPQICFQEDVLPLMV
                     SSCTQSGCHNPYDLKKGYDFSNYEGIVKAVKKGDYRASKLYKSIISESADRIPQKPYP
                     ALTDEQVKTVALWIEQGALNNLSCHSACDTTMARYSAEVKPVLNKYCSGYHGNSPYQG
                     GIKLSGYNDVVQYAQNGRLLGSIKFEPTFSPMPKNSNQIPQCKIDIIEKWIKEGAQNN
                     "
     gene            2054..2932
                     /locus_tag="PLE23_11615"
     CDS             2054..2932
                     /locus_tag="PLE23_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HMX83957.1"
                     /translation="MKQLILIPVIIFISNLSLLSQDNGSLLDALGSTDETIYIDNTFK
                     SPRVINSHSVEMLGKGVLDFRILHRFGPLKDGLYEMFGLDHATMRMSFDYGVTNHLTL
                     GVGRSTRNKEFDIMAKYRILQQSTGAREMPVSVIWASGAMINTLKDPFAGDLKPNFSR
                     RLSFYHQLIVGRKFSDAFSLQLSPILVHRNDTQSCLDPNLLFGLELGTRYKLTNRFAL
                     VLDYTHTFNRFPNEEGANPLSIGVDIETGGHVFQLHFSNALGMNERSTTTDNNQSWLK
                     GNFMFGFNLSRFFQLN"
BASE COUNT          951 a          714 c          586 g          884 t
ORIGIN      
        1 ctcccgtatc agattccccg ccgcatcaaa tatcttaaca ttgctggcta tgcgtttaga
       61 gtattcgtac cctaacaaag tcatattggt ataaacaaat gcacatgaat taaaaatatc
      121 agtatacata gaaatcctta ctcccgtatc ctcggggtct tctcctaaat cacccaagga
      181 gtttatgtag gggattttct ttgacttttg gtttatcctt atcattacag aattattccg
      241 ccagtcacac ctttcaggta tcgtgtcaaa ttcataattc cggaatgcct gcagctcttt
      301 aaaagaaaga aacttaagcg gtacccggtt agctgtatct ccataaaagt taaggcctaa
      361 gcttctgcca tatctttccc cgtctgtctt tatttctatc ttcttccttt gtccaaaggc
      421 agaaaccgaa aaaaccaaca aacaaaaaaa cagtaatatt ctcattcttt tttctttagt
      481 gctgtaattt gttcatcgag gctgatcatg tacagggtca gctcttcgac ttttttgagg
      541 aggagctttt gggtctcgaa aacatccatg ccattggcgt tgatgtcagc ttcggtcggc
      601 acttccggca ggtgcttgtt ggtcataaca tacgacttaa gctcagccag cgacatgagc
      661 ttatagtcag gctcgaatac ataatcgcac caaccatagt tacgtatagt aacttcttta
      721 cattccacct tgccgttgcc atataccctg aaggtctcac atgaggaacc attattagca
      781 agtgatactg aaaaagcttt agtctgggga ttataaactt tgctgaggat tccgactccg
      841 ctaccaaaat agtcagaata aatgcctacc ttaaggcctg actctactgt caacatacaa
      901 tcaccctgcg gttgtgcgac accgatacct acctttttgg atatatctgc aggcactaaa
      961 ccgttgggca gtgaattaat acccactatt ttccaaaagc acgtagtggg ttgggctacc
     1021 aatgataatg taaaaaagca attaataaaa agtaaacaat aaagccgggt tgttcgttgc
     1081 gatttcataa attgatacat tttttacaaa gatagtgttt tattttaaat tacaaaaaaa
     1141 tatttctttt ttgagtatta attttttata ataatgtttt tccgtatata ataatttgta
     1201 aacatgtctt ccaaacgcac aaaacagacc gaaatggttt caacctctta atgttttatc
     1261 atattttttt aaaagaatta ttccctgaca aaaaaatcaa aatccttggt taatgataaa
     1321 aaattaaaca gaaagtttta ttcgttttaa ctttgccatt attttattaa atatcatgaa
     1381 aaaatattca ttattagtat cacttttgct ctcaggaatc ataatcttcg ggtttcagaa
     1441 ttgcaaaaag gaacaccttc ccgacccgca gatatgcttc caggaggatg tgctgccctt
     1501 gatggtaagt agttgcacgc agagcggttg ccacaatccg tacgacttga aaaaaggcta
     1561 tgactttagt aattatgagg gaattgtgaa ggccgtaaaa aagggagatt accgggcaag
     1621 taaactatac aaatccataa taagtgaatc ggctgatcga atacctcaga agccatatcc
     1681 ggccctgaca gacgagcagg taaagaccgt cgccctctgg atcgaacaag gagcacttaa
     1741 caacctgagc tgccattctg cttgtgatac tacgatggcc aggtactcag ctgaagtaaa
     1801 gcccgtactc aacaaatatt gcagtggtta tcatggtaac agtccgtatc aggggggcat
     1861 taagctttcc ggctacaacg atgttgtaca atacgcacag aacggaaggt tgctgggttc
     1921 aataaaattc gaaccaacat tctctccgat gccaaaaaac agcaaccaaa taccacagtg
     1981 caaaatcgat attattgaaa aatggattaa ggaaggtgca caaaacaatt aaacaaacca
     2041 attatcaaac aaaatgaaac agctaatact aattccggtt ataattttta tttcgaacct
     2101 tagtttatta tcacaagata acggcagcct gctggatgct cttggctcca ctgacgaaac
     2161 catctacatc gacaacacct tcaaatcgcc aagagtcatc aacagccaca gcgtagagat
     2221 gctcggcaaa ggcgtactcg acttccgtat tctccacagg tttggccccc ttaaggacgg
     2281 tctgtacgaa atgttcggcc ttgaccatgc taccatgcgt atgagttttg actacggtgt
     2341 cacgaaccac ctcacattag gtgtaggtag aagcacacgc aacaaagagt ttgacataat
     2401 ggccaaatac agaatcctac aacagtccac cggagcacgc gagatgcctg tgagtgtcat
     2461 ctgggccagc ggagccatga tcaatacact caaagacccg tttgccggtg accttaaacc
     2521 gaacttttca aggaggcttt cattttatca tcaactcatc gtcggtcgga aattcagcga
     2581 cgctttctcg ctccaactgt caccgatact cgtccatagg aatgacaccc agtcttgtct
     2641 cgacccaaat ttattgttcg gcctcgaact cggtacacgc tacaaactca ccaacaggtt
     2701 tgcacttgtg cttgattata cccatacatt caatagattc ccaaatgaag aaggcgctaa
     2761 cccactttcc atcggtgtcg atatcgaaac cggcgggcac gtcttccaac tccatttttc
     2821 gaatgcactg ggcatgaacg aacgttcaac taccaccgac aacaaccaaa gctggcttaa
     2881 aggcaacttc atgttcggat tcaatctgtc aagatttttc cagttgaatt aatgccgcat
     2941 caacatcata aaaattggct ctgcgaagat agcagagcca ttttttttta aactccgggc
     3001 aaccggtatc agcagtacga gcttttgaaa aatacctctt agcatagatt ttttcattag
     3061 ggcgtacccc tgtcgggtcg ggctatccgc cttacttcgt gccggcttct atcccttacg
     3121 cagatggtaa gtggt
//
LOCUS       DAOQQY010000308         3134 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_15841_length_3134_cov_5.551478, whole genome shotgun sequence.
ACCESSION   DAOQQY010000308 DAOQQY010000000
VERSION     DAOQQY010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15841_length_3134_cov_5.551478"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..1579
                     /locus_tag="PLE23_11620"
     CDS             131..1579
                     /locus_tag="PLE23_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMX83958.1"
                     /translation="MRILLTGANGYIGMRLLPVLVEAGHQVTCVVRDKNRFRPSEDLL
                     EKVEIIEFDFLNPEFALRKFSKKEFDAAYYFIHSLGDTSTTLKEYEVRCAGTFVLVAS
                     LTKVKQIIYLSGISNDSELSKHLNARKSVKDVLINSDIPYTIFEAGVIVGSGSISFEI
                     IRDLTEKLPLMIVPKWLNSKCQPIAIRNVINYLHLCLLNEKTYNRTFEIGGPDVLTYK
                     QMLLEYAKVRGYKRYIVTMPVIFPGLSAHWINASTSANFTIAKQLVQSMKNDVVCKDF
                     SIRDIIPQQLYTYEEAIKMAFARIEQNMVVSSWTDSASSSLTNLDVNQHIEVPTNGCY
                     KDKKWIKIDCTKTEEVAARFFGIGGEHGWYYADTLWRIRGVLDKFMGGVGISRGRRSK
                     TDIEAGDTIDFWRVILADKKHHRLLLYAEMKLPGEAWLEFSIVKIKNQCILKQTATFR
                     PTGIMGRNYWYSMLPFHYFIFRNMLKRIAGVK"
     gene            1688..2422
                     /locus_tag="PLE23_11625"
     CDS             1688..2422
                     /locus_tag="PLE23_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMX83959.1"
                     /translation="MTPDILFYLLLFMVALLYASVGHGGASGYLALMAFYALSPDTMR
                     PTALVLNIFVSGTSFFQYYRGGHFRWRLFWPFALASIPAAFLGGMVSIDAGLYKKILG
                     VLLLFAIARLTGVGIFRENETEKPQNLFLSLVVGLVIGLFSGMIGIGGGIILSPVILL
                     LHWADMKKTAAVSALFILVNSVAGISGMFTQGIQFNTAMATMVMVALAGGLTGSYMGA
                     RKIGNRMLRNILAPVLLLAAVKLLLT"
     gene            2442..3041
                     /locus_tag="PLE23_11630"
     CDS             2442..3041
                     /locus_tag="PLE23_11630"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HMX83960.1"
                     /translation="MYSKYPNISAYVLAGGKSSRMGADKGLVLFREKPMVQYVLDVLD
                     ANFTKTTIISPNQDYEQFGYEVIADIIPDIGPAGGIYTALKHTSTKKIFIISCDMPFI
                     TSKVVSFLVKNSRSAQICLSLSDDYIQPLPGVYSTECAEKWEELVKQGNYKLRDLVTH
                     FRLKTIETKNMFGISDVLFMNINDKKQLEHALNLYIDEN"
BASE COUNT          959 a          648 c          667 g          860 t
ORIGIN      
        1 atatattggc caattgtttt atttttggca agataagaag attgagaaaa gcaacagtta
       61 tacttcatgt tgaagctgtc ctcaagctta tgtcctttaa ttactaacac aaaactaaaa
      121 ccaacccaac atgcgcattt tactaacagg tgccaacgga tatataggaa tgagactctt
      181 accggtattg gtggaggcag ggcatcaagt cacttgtgtg gtcagggaca aaaaccgatt
      241 cagaccttca gaagatttac tcgaaaaggt tgaaatcatc gaatttgact ttctgaatcc
      301 tgaatttgca ctccggaaat tcagcaaaaa agagtttgac gccgcctact attttatcca
      361 ctcacttggc gacacatcga ctacactgaa ggaatacgaa gtaagatgtg ccggcacatt
      421 tgtactcgtg gcatcgctca ccaaggtaaa gcaaatcatc tacctgagcg gtataagcaa
      481 tgacagcgaa ctgtcaaaac atttgaatgc ccgcaaaagc gtaaaagatg tattgatcaa
      541 ttcggatata ccttacacca tttttgaagc aggcgtcatt gttggctcgg gaagtatatc
      601 attcgaaata atacgtgacc tgaccgaaaa actgcctcta atgatagtgc ccaaatggtt
      661 gaactcaaaa tgccagccca tcgccatcag gaatgttatc aactaccttc atctatgttt
      721 attgaatgaa aaaacataca accgtacttt cgaaattggc ggaccggatg tattaaccta
      781 caaacagatg ctgctcgaat atgccaaagt acgtggatat aaacgttaca tagttaccat
      841 gccagtcatt tttccgggtc tttcagcgca ttggatcaat gcttcgacat ctgccaattt
      901 tacaatagca aagcagcttg tacagagtat gaaaaacgat gtagtgtgca aagatttctc
      961 cattcgcgat attatacccc agcaattata cacatacgaa gaggctataa agatggcttt
     1021 tgcaagaata gagcaaaaca tggtagtatc gagctggaca gactcggcct cgagcagcct
     1081 tactaatctt gacgtaaacc agcacataga agtacctacc aacggatgtt ataaggataa
     1141 aaaatggata aagatagact gcaccaagac agaggaggtg gcagcacgtt tttttggcat
     1201 cggtggagaa cacggctggt attatgccga cacactctgg cgcatacgtg gcgtacttga
     1261 caaatttatg ggaggtgtgg gcataagcag aggccggcga agcaaaacag atattgaagc
     1321 cggtgatacc atcgacttct ggcgcgtaat attggccgat aaaaaacacc atcgcttgct
     1381 cttgtacgcc gaaatgaagc tacccggaga agcctggctc gagttttcga ttgtgaaaat
     1441 caaaaaccag tgcatactaa aacaaaccgc tactttccgc ccgacaggta tcatgggtag
     1501 aaattattgg tattcgatgc tgccgtttca ttattttata ttccgaaata tgctaaaacg
     1561 gatagcaggt gtaaaataag ctacatatat atatgtatgt atgttgagct tgatttcagg
     1621 caggctgata agtaataatg cttatatttg taacgaaagc ttattaactg aaataaaaaa
     1681 tcataatttg acacctgata tcctattcta cctattgttg ttcatggtag ccctactgta
     1741 tgcttccgta gggcatggtg gtgccagcgg atatcttgca cttatggcat tttatgcttt
     1801 atcgccagac accatgcggc ctacagccct tgttctcaac atttttgtgt caggtacatc
     1861 attttttcaa tattaccgtg gcggacattt caggtggcga cttttttggc cttttgcact
     1921 cgcttccatt ccggctgctt tcctgggtgg catggtcagt attgatgcag gtctttacaa
     1981 gaaaatactc ggtgtgcttt tgttgtttgc tattgcaagg ctcaccggtg tgggaatatt
     2041 ccgggaaaat gagactgaaa aaccacaaaa tctcttcttg tcactggtag tcggactggt
     2101 catcggatta ttttctggca tgattggtat tggtggcggt attatcctta gtccggtgat
     2161 cttgttactg cattgggccg acatgaaaaa gactgctgca gtatcagcac tatttatttt
     2221 ggtcaattcc gtagcaggca taagtggaat gtttacccaa ggaatacaat tcaatacagc
     2281 aatggctact atggttatgg tagctctagc aggcggactc acaggttcgt acatgggcgc
     2341 ccgaaaaata ggcaacagaa tgctcagaaa catactggcc cctgttttat tattggctgc
     2401 agtgaaatta ttgctaactt agccctgtaa tacattcaga catgtacagc aagtacccga
     2461 acattagtgc ctacgtcctc gccggcggaa aaagcagccg catgggcgcc gataaaggtc
     2521 ttgttctatt tcgcgaaaaa cctatggtac aatatgtact ggatgtgttg gatgcaaatt
     2581 tcaccaaaac aaccattatc agccctaacc aggattatga acagtttggg tatgaagtga
     2641 ttgccgacat cattccggat atcgggcctg caggtggtat ttacacagct ctgaagcata
     2701 caagtacgaa aaaaattttc atcatcagct gcgatatgcc tttcattaca agtaaggtag
     2761 tcagtttttt agtgaaaaat tcgcggagcg cccaaatatg cttaagcttg tctgatgatt
     2821 acatccaacc ccttccaggt gtgtatagta ctgaatgtgc cgaaaaatgg gaagaactcg
     2881 taaaacaggg aaactacaaa ctccgcgatc tggtgacgca tttcagactc aaaaccattg
     2941 aaacaaaaaa catgtttggc attagtgatg ttctctttat gaatatcaat gacaaaaaac
     3001 aactcgaaca cgcccttaat ctatatattg atgaaaatta aattactcgc atttggacaa
     3061 atagccgaaa taaccggaga acaaaccgaa ctcgcagtag atgtatgtga tactaccgaa
     3121 ctgctgcaac tact
//
LOCUS       DAOQQY010000309         3080 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_16301_length_3080_cov_6.174545, whole genome shotgun sequence.
ACCESSION   DAOQQY010000309 DAOQQY010000000
VERSION     DAOQQY010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16301_length_3080_cov_6.174545"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="PLE23_11635"
     CDS             complement(<1..513)
                     /locus_tag="PLE23_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMX83961.1"
                     /translation="MDIKLKRDLCFFDIESTGLNVIKDRILQLAIIKLKADGSPEEEL
                     CLLINPGFPISEEAMAIHGITPKDLINKPTFEQVAQRIFDFIGDSDLAGYNCHRFDVP
                     MLMEEFARVGCEFDIDRRKVIDVQRIFYKMEPRTLKAALKFYCDQDIEDAHDALADVR
                     ATIEVFKGQLD"
     gene            complement(541..1383)
                     /locus_tag="PLE23_11640"
     CDS             complement(541..1383)
                     /locus_tag="PLE23_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMX83962.1"
                     /translation="MMDFLLFMDFTSVDAWMALLTLTFLEIVLGIDNIIFISIAANKL
                     PEKEQPRARFIGLGLALVFRIIMLLGLSFLIGLKEPFWSFHSDYISVALNGQAVILFL
                     GGIFLLYKSVAEIHHKLEGDPEDDKVKIKGAGSKLMNVIVQIAMIDMVFSIDSILTAV
                     GLTKHVSIMIAAVIISIAIMLVFAGPVGRFINKHPTIQMLGLAFLLLIGFMLITESAH
                     LSHFKLGDEEVGSIPKGYLYFAIAFSVMVEFLNSKLRKKPVPPVDLRSINEEAQNEGL
                     LDKM"
     gene            complement(1411..2085)
                     /locus_tag="PLE23_11645"
     CDS             complement(1411..2085)
                     /locus_tag="PLE23_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83963.1"
                     /translation="MKKILFFTLLLIYSYIGIAQECHDYFPFKAGTEWEMTNYDAKNK
                     VLTVSEYKIESIHSNVAEIHLTSKDAKGKQLADMRLKWYCDEEKVNFEMKDMFPQMDM
                     MDNANVEMKFSGDNLSYPKNMKAGDLLPDAESKIEMMMNGMRVMSMVVKVTDRKVEGT
                     EQLSTPAGSFECLKLSETAEVKSIMSAKTKSVIWLSKNRGVVKTDSYNSKGKLEAYQL
                     MTKFKS"
     gene            complement(2567..2818)
                     /gene="rpmA"
                     /locus_tag="PLE23_11650"
                     /pseudo
     CDS             complement(2567..2818)
                     /gene="rpmA"
                     /locus_tag="PLE23_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
     gene            complement(2850..>3080)
                     /gene="rplU"
                     /locus_tag="PLE23_11655"
     CDS             complement(2850..>3080)
                     /gene="rplU"
                     /locus_tag="PLE23_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMX83964.1"
                     /translation="KEGDKVSFDQVLLVSSGSDVTLGKPSVAGAKVNATVLKHAKGDK
                     VIVFKKKRRKGYRVKNGHRQCFTQIKIDSITR"
BASE COUNT          867 a          683 c          556 g          974 t
ORIGIN      
        1 atccaactgt cctttaaata cttcaatggt ggcccgcaca tcagccagcg cgtcgtgagc
       61 gtcttcaata tcctgatcac aataaaactt aagcgctgct ttcagggtgc ggggctccat
      121 tttataaaaa atgcgctgta catcaatcac ctttcttctg tctatatcaa attcacaacc
      181 tacgcgtgca aactcttcca tcagcatagg cacatcaaat ctatgacaat tgtagcctgc
      241 caggtcactg tcaccaatga aatcaaatat gcgctgtgca acttgttcga aagtaggttt
      301 attgataaga tctttaggtg ttatgccgtg gatggccatt gcttcttccg aaatagggaa
      361 acccggattg attaacagac acaattcttc ctcaggcgaa ccatcggctt taagcttgat
      421 gatagcgagt tgtaaaatcc tgtcctttat gacattgagg ccggtcgatt caatatcaaa
      481 gaagcaaagg tcgcgtttta gtttaatgtc cactgtgcaa gaaaattttc ggaataaata
      541 ttacatttta tcaagaaggc cttcgttttg tgcctcttca ttaatagacc gtagatcgac
      601 cgggggaacc ggcttcttac gcaattttga attgagaaat tccaccatga cgctgaaggc
      661 tatggcaaaa tacagatagc cttttggtat tgagcctact tcttcgtctc ctaatttgaa
      721 atggctgagg tgtgcacttt ctgtaatcag cataaaacca atcagtaaca gaaatgcaag
      781 accgagcatt tgtatagtag gatgtttgtt tataaatctg ccaaccggac cggcaaaaac
      841 gagcatgatg gcaatagaaa taatcactgc tgctatcatt attgatacat gctttgtaag
      901 gcctactgct gtaagtattg aatcaatgga gaagaccata tctatcatgg caatctgaac
      961 gattacattc ataagctttg atccggcacc tttgattttt accttatcat cttcagggtc
     1021 gccttcgagt ttgtggtgaa tttcagccac actcttgtat aaaaggaaaa ttccgcccag
     1081 gaataaaata acagcctggc cattgagagc tacagagata taatctgaat gaaatgacca
     1141 gaatggctcc ttaagtccta ttagaaaaga aaggcccaac aacatgatga tcctgaatac
     1201 cagtgccaac cccaaaccaa taaaccttgc acgtggttgt tctttctccg gcaatttatt
     1261 agctgcgatg gagataaata ttatgttgtc aatgcccagt actatctcca gaaatgtaag
     1321 cgtgagtaat gccatccagg catcaacact tgtaaaatcc ataaaaagca gaaaatccat
     1381 catataaagt gtttgtctta atgatatatt ttatgattta aatttagtca taagctgata
     1441 agcttcgagc ttgcctttac tgttatacga gtctgttttg accacacctc tatttttgct
     1501 aagccaaatg acagattttg tcttggccga catgatagac ttcacttcag cagtttcgct
     1561 tagtttcagg cattcaaacg aacctgcagg tgttgataat tgctcggtgc cttctacctt
     1621 tcggtcggta acttttacaa ccatactcat cacccgcata ccattcatca tcatttctat
     1681 ctttgattct gcatccggca acaggtctcc tgccttcata ttttttggat aagacagatt
     1741 atcgcctgaa aatttcattt ctacatttgc attatccatc atatccatct gagggaacat
     1801 atccttcatc tcgaaattga ccttttcttc atcacagtac catttgagcc tcatatccgc
     1861 caattgtttg cctttagcgt ccttagatgt aaggtgtatt tcagctacat tcgaatgaat
     1921 actttcaatc ttatattcac tcacggtcag aactttgttt tttgcatcat aatttgtcat
     1981 ttcccattca gtacctgcct taaatggaaa ataatcatgg cactcttgag caatgccaat
     2041 atatgaatat atcaacagta aagtaaagaa cagaatcttc ttcatgacag ttaatttatt
     2101 gagagcaaac ctaataaaat tctgacaata aaaaaaggca gttcttttca tgaaccacct
     2161 tttttgaata tttttataca aatgtgaaat attattccac tccgcctttc agttgatctt
     2221 gaagagccag tagttcgtcc catttgcctt cgtgagctag caatgcctgc ttcgcccaag
     2281 tgcccgggtt atgactcttg tacctagggc ctgctgcttc gagaatggct tcgagttttg
     2341 tcaggtctgc atttgcaaga tctgagaaag tatgaatacc tgcttcctga agcaatactt
     2401 cgatcttagg gccgatgcct tccaccattt tcagattatc gcctgattca gctgattttg
     2461 ctgctttagc cggaactgtt tctggttttg aatcttctac cactggagca gcggctgcag
     2521 gttttgcttt tttgacaggt gcaatggttt cttttacctc ttcaaactta ggcattacag
     2581 aaacgaaggt tctgcccaat tttcttttag taaatgtcac aacaccttca gccagagcaa
     2641 aaagagtatg gtctctaccg atgcctacac ctgcaccggg atgaaatctc gtacctcttt
     2701 gacgaataat gatattaccg gggatggcgt gttgtccgcc aaaaagttta acaccaagtc
     2761 gtttactttt actatccctt ccgttatctg aactaccaac acctttctta tgtgccatga
     2821 ttctttaaat ttttgagtgt tcgaaaaaat tatctggtta ttgaatcaat cttaatctga
     2881 gtaaaacact gacggtgtcc gttctttacc cggtaccctt tacgtctttt cttcttaaaa
     2941 acgattactt tatcaccttt ggcatgcttc aaaaccgtag catttacttt tgcgcctgct
     3001 acagatggct taccaagtgt gacatcactg ccagatgaca ccaggagaac ctgatcaaag
     3061 gaaaccttgt ctccttcttt
//
LOCUS       DAOQQY010000310         3071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_16390_length_3071_cov_5.569960, whole genome shotgun sequence.
ACCESSION   DAOQQY010000310 DAOQQY010000000
VERSION     DAOQQY010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16390_length_3071_cov_5.569960"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PLE23_11660"
     CDS             complement(<1..818)
                     /locus_tag="PLE23_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HMX83965.1"
                     /translation="MRQLKITKSITNRESQSLEKYLQEIGKVDLLTPEEEVDLAKKIK
                     DGDQIALERLTKANLRFVVSVAKQYQNQGLSLSDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKVGSLNKINKAFSELEQAHEREPSAEELAE
                     LLEIPTEEVETTLGVAARHVSMDAPFVEGEDNSLLDVLENNTSPGTDTALEYLESLRR
                     EIERSLGTLTDRQADVIKLYFGIGIEHPMSLEDIGDKFGLTRERVRQIKDKAIN"
     gene            1221..1601
                     /locus_tag="PLE23_11665"
     CDS             1221..1601
                     /locus_tag="PLE23_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HMX83966.1"
                     /translation="MKRVQLKLDFILKASPTIVYNFLTTPACLVRWFCDRVDIEGEIY
                     TFEWNKSREWAELIDDIEEEYLRFRFDSAENDDEYLEFRITTSPITDETILEIKDWCD
                     ENELADQKLYWENLIVRMQREMGA"
     gene            complement(1746..>3071)
                     /locus_tag="PLE23_11670"
     CDS             complement(1746..>3071)
                     /locus_tag="PLE23_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX83967.1"
                     /translation="PDADNSFILSGLGYGSGPGESTWYHNYSTTMWSGSLQLVMSINQ
                     FRFIKPVRTINPYVFAGAGLGSYKVKTDAKDANGNIYNFTTVPSESDLDGTYETELAI
                     QQKDLGKTLINPMGEAGVGIAFRITPRFNIGIEHKMGVLLGKRGDYVDGAVFRSSVDQ
                     TNNKDLIHSTYIRLNFNIGNKEKMSEPLWWVNPLDFVLKDIAELKERPKLDLTDTDKD
                     GVLDMFDQEKETPAGAPVDVRGMALDSDGDKIPDYKDKEPYSAPNYQVDADGRAMVPK
                     NSVSPEEVNKLIEDKLKEFNITKGGTLTDWFLPAIHFDLDKYTIKNSEAEKLRQVAVV
                     MRNNTSLKVMVTGHTDKTAGASYNKMLSYNRSKAAIDYLVNKYGIARNRFVLTYSGKE
                     DTLIDTQAANYMNRRVEFRVAKPGDVEMPKPSGPNAGSGKFEGSRDAGY"
BASE COUNT          845 a          604 c          596 g         1026 t
ORIGIN      
        1 ttattgatag ccttgtcttt tatctggcgg actctttcac gtgtcagtcc aaatttatca
       61 cctatatctt ctagtgacat tgggtgttcg atgccgatgc cgaaatacaa tttgattaca
      121 tcagcttgtc tgtctgtcag cgtacctaat gatcgttcga tttctcttct aagggattca
      181 aggtattcga gggcagtatc tgtaccaggt gatgtattgt tttccaaaac atcgagaagc
      241 gaattgtctt caccttcaac aaaaggtgcg tccattgaca cgtgccttgc agctacaccc
      301 agtgttgttt ctacttcttc tgtcggtatt tcgagcaatt cggctagttc ttctgcagat
      361 ggttctcgtt cgtgtgcttg ttcgagttcg gagaatgctt tgttgatttt gttaagagag
      421 cctactttgt tgagtggaag tctcacaata cggctttgtt cggccagtgc ttgtaaaatg
      481 gattgacgaa tccaccaaac ggcataagag atgaatttga atcctcgtgt ttcgtcaaat
      541 ctttgagctg cctttatcag accgaggtta ccttcattta tgaggtcact aagggaaagc
      601 ccctggtttt gatattgttt agccaccgaa accacgaatc ttaagttggc ctttgtcaat
      661 cgttcgagtg caatctggtc tccgtctttg atttttttgg cgaggtccac ttcttcttcc
      721 ggagtaagca aatccacttt gccgatttcc tggaggtatt tctctagcga ctggctttcg
      781 cggttagtga ttgattttgt aattttcagt tgtctcatgc aatagttttt ttatgcttca
      841 ctaatgaatt cagatcggca caatggccgt ctgggagatt tttctgatat attgggattg
      901 gaacgataat ttgctggtat attatttata cggaaatatt gaaaaaagtt gccatttgaa
      961 gatattattt tcacctgcct gcaaaattaa agcaaaattc aattgttgtc aatagtagcc
     1021 tttgtatttg aaaattatac ataattacat tcgttaatat aatatttctg actggctcaa
     1081 aaccattgtg ttatagattt attaaaaaaa attattaaaa aaataactga aataaatttt
     1141 tattttttgc ctgaggtatt acattgcgaa caaaacagac taagatttat ttatttatat
     1201 tagctaacaa ccgctatatc atgaaaagag ttcaacttaa actggatttt attttgaaag
     1261 cttcacctac gattgtgtat aattttctta ccacacctgc ttgtttggta aggtggtttt
     1321 gtgaccgtgt ggatattgaa ggtgaaatct atactttcga atggaataaa agcagagaat
     1381 gggccgagtt aattgacgat atcgaagaag aatatctcag attccgattt gacagtgccg
     1441 aaaatgacga cgaatatctt gagttcagga ttacaacctc acctatcaca gacgaaacta
     1501 ttcttgaaat aaaggattgg tgtgatgaaa atgaacttgc cgatcagaaa ctgtactggg
     1561 agaacctgat tgtgagaatg caacgtgaga tgggcgcata attttttcta tcatacttgg
     1621 ataacgtact ttttgactga acaagcaaaa aagaaagtag aagaataaaa aaagcaattg
     1681 aataaaaaaa ctgccgtcaa caaagtgtca gcggcagttt tttatttaaa tttattcaca
     1741 ttatactaat agccggcatc cctactacct tcaaattttc ctgatccggc atttggtcca
     1801 cttggttttg gcatttcaac atcaccgggt ttggctactc tgaattctac ccttctgttc
     1861 atgtaattag cagcttgcgt atcgatgagt gtatcttctt tgcctgaata ggtaagtaca
     1921 aatctgttcc ttgcaatacc atatttgtta accagataat caatggcagc ttttgatcgg
     1981 ttatatgaca acattttatt gtaggatgct ccagctgtct tgtctgtatg accggtcacc
     2041 atcaccttca gagatgtatt atttctcatt actactgcca cctgacggag tttttcggcc
     2101 tccgaatttt ttatagtata tttgtcaagg tcaaaatgaa tagcaggcag gaaccagtct
     2161 gtaagtgtac caccctttgt gatattgaat tccttaagtt tgtcttcgat gagcttattg
     2221 acctcttccg gagatacaga attttttggt accatcgccc taccatcagc atctacctga
     2281 tagttaggtg cggaatacgg ttctttgtcc ttgtaatcag gtatcttatc accatcactg
     2341 tctagtgcca tgccacgtac atctacaggt gcgcctgccg gtgtttcctt ttcctgatca
     2401 aacatatcaa gaactccgtc tttatctgta tcggtaagat caagttttgg cctttccttt
     2461 agttcggcaa tatctttcag tacaaaatca agcggattga cccaccaaag tggttcagac
     2521 atcttctctt tgttgccaat attgaaattt agcctgatat atgttgaatg tattaagtct
     2581 ttgttgttgg tttggtcaac cgaggatctg aatacggctc catctacata atcaccacgt
     2641 ttaccaagca gaactcccat tttgtgctca ataccgatat tgaagcgtgg cgttatcctg
     2701 aaggctatac ccactccggc ttcacccatc gggtttatca gggttttgcc caaatctttt
     2761 tgctgaatgg caagttctgt ctcgtaagta ccatcaaggt cactttcaga aggaacggta
     2821 gtaaaattat atatattgcc attagcatct tttgcatctg tttttacttt ataagaccca
     2881 agaccagctc ctgcaaacac ataagggttg attgttcgca caggcttaat aaacctgaac
     2941 tgattaatag acataaccag ttgcaaactt cctgaccaca tcgtagtaga ataattatgg
     3001 taccaggttg actctcccgg gccactgcca tagccaagtc cggacaaaat aaaagaatta
     3061 tcagcatcgg g
//
LOCUS       DAOQQY010000311         3038 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_16670_length_3038_cov_5.886691, whole genome shotgun sequence.
ACCESSION   DAOQQY010000311 DAOQQY010000000
VERSION     DAOQQY010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16670_length_3038_cov_5.886691"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLE23_11675"
     CDS             <1..217
                     /locus_tag="PLE23_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMX83968.1"
                     /translation="EDPKFLTVTPVFLAANWMERETYDFYGAIFEGHPDLRRILNMDE
                     MHYFPMRKEYPLQDATRTDKPDKYFGR"
     gene            174..1415
                     /locus_tag="PLE23_11680"
     CDS             174..1415
                     /locus_tag="PLE23_11680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMX83969.1"
                     /translation="MQQELTNQTNISAGNLPADQKEKLQVQDYNTLNLGPTHPATHGI
                     FQNVLKIDGEKILDAEPTIGYIHRAFEKIAENRAFYQVTVLTDRLNYCSSPINNMGWH
                     MTVEKLLGVKTTKRVDYMRVIIMELSRIADHLICNSVLGVDTGALTGFTYVFQLREKI
                     YDIFEEVCGARLTTNIGRIGGMERDFTSKFWSLLKEFMDEFPKVFKEFDLLFTRNRIF
                     IDRTKNVGGISAERALSYGFTGPNLRAAGVDYDVRVMNPYSSYEDFEFDVPVGDTGDC
                     YDRYLVRNEELRQSYNIIKQAMENMPEGRHYVDVPEFYLPPKQDVYTKMEPLIWHFKI
                     IMGEWPVPVGEVYHPVEGANGELGFYLVSDGGRQAYRLHFRRPCFIYYQAYPELIKGG
                     MLSDAIVTMSSLNVIAGELDA"
     gene            1428..1958
                     /gene="nuoE"
                     /locus_tag="PLE23_11685"
     CDS             1428..1958
                     /gene="nuoE"
                     /locus_tag="PLE23_11685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMX83970.1"
                     /translation="MSKITFSEETLKTVREIIARYPEGKQKSALIPLLHIAQSEFDGW
                     LSPEVMDYIADILHIKPIEVYEVASFYTMFNLKPVGKCLIEVCQTSSCWLMGANEIVE
                     HIYKRLGIKPGETTADGMFTLKTVECLGSCGTAPMLMIGADYHENLTFEKVDNLIEKC
                     RQSGKRSSYLVDNMLG"
     gene            1979..>3038
                     /locus_tag="PLE23_11690"
     CDS             1979..>3038
                     /locus_tag="PLE23_11690"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762797.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HMX83971.1"
                     /translation="MGKKLLLKNIDIPGISSYEVYRREGGYKSVEKVLAEKNPEAVVE
                     EVKKSGLRGRGGAGFPTGMKWSFLAKPEGVPRYLVCNADESEPGTFKDRYLMEHIPHL
                     LVEGMITSSYALGANKSFIYIRGEYFYVLRILEKAIKEAYDNGWLGKNIKGSGFDHDL
                     YVQIGAGAYICGEETALLESLEGNRGNPRIKPPFPAVAGLYGCPTVVNNVETIAAVVP
                     IINDGGEAYAAIGVERSTGTKLISASGHIKKPGVYEIELGLPVEEFIFSDEYCGGIIN
                     GRKLKAVVAGGSSVPVLPAELILKTAAGTPRIMTYESLADGGFATGTMLGSGGFIVMD
                     ETASVLRNLHTFARFYHHE"
BASE COUNT          936 a          561 c          696 g          845 t
ORIGIN      
        1 cgaagatcca aaatttctta ctgtgacacc ggtattctta gccgctaact ggatggaacg
       61 tgaaacatac gatttttatg gtgccatatt tgaaggtcat cctgatcttc gtagaatttt
      121 gaatatggat gagatgcatt atttcccaat gagaaaagaa taccctttac aagatgcaac
      181 aagaactgac aaaccagaca aatatttcgg caggtaattt gcctgctgac caaaaagaaa
      241 agcttcaggt tcaggattat aatacgctga accttggtcc gacgcatccg gctacacatg
      301 gtattttcca gaatgtgttg aaaatagacg gagaaaaaat tcttgatgca gaaccaacta
      361 taggttacat ccaccgtgct tttgaaaaaa tagctgaaaa cagagcattc tatcaggtaa
      421 cggtacttac agaccgactc aattattgct cctctcctat caataacatg ggttggcaca
      481 tgacagtaga aaaactgctc ggtgtaaaaa ccaccaaacg tgtagattac atgcgggtca
      541 tcattatgga actttcccga atagctgacc acctgatttg caactcggtt cttggtgttg
      601 atacgggtgc gcttaccggc tttacttatg tattccagct gcgagaaaag atctacgata
      661 tttttgaaga agtctgcggg gcaaggctta ctactaatat tggtcgaatt ggtggaatgg
      721 aacgtgattt tacgtcaaaa ttctggtcac tccttaaaga atttatggat gaatttccaa
      781 aggtttttaa agagtttgac ttacttttta ccagaaacag aatattcatt gaccgcacaa
      841 aaaatgttgg cggtataagt gcagaacgcg ctttgagcta cggctttact ggcccaaatc
      901 tgcgagcagc aggtgtggat tatgatgttc gggtcatgaa cccttattca tcgtatgaag
      961 attttgaatt tgatgttcct gttggcgata caggtgactg ctatgataga tatctggtca
     1021 gaaatgaaga attaagacag agctacaata ttattaaaca ggcaatggaa aatatgccgg
     1081 aaggcagaca ttatgttgat gtgccggaat tttatttgcc gccaaaacaa gatgtttaca
     1141 caaaaatgga gccacttatc tggcatttta agataatcat gggcgaatgg cctgtgccgg
     1201 tcggcgaagt ttatcatcct gtagaaggag ccaatggcga actcgggttt tatctggtaa
     1261 gcgatggtgg acgtcaggca taccgattac atttcagaag accttgtttt atttattatc
     1321 aggcctaccc cgaactcatc aaaggaggaa tgttgtcaga tgccattgtg accatgagta
     1381 gccttaatgt gatagcagga gaattggacg cttaaagaaa aatgaacatg agtaaaatta
     1441 cattttcaga agagacgcta aaaactgtca gggaaatcat cgcaagatac cccgaaggta
     1501 aacaaaaatc tgctttgatt ccactattac acattgctca gtcggagttt gatggctggc
     1561 ttagtcctga agtaatggat tatattgctg atattttgca catcaaacca atcgaagtct
     1621 atgaagttgc atcgttttat actatgttta accttaagcc tgtcggcaaa tgtttgattg
     1681 aagtatgcca aacgagttct tgctggctta tgggtgccaa cgaaatagta gaacacatct
     1741 ataaaagact aggtataaaa cccggcgaaa ccactgcaga cggaatgttc accttgaaaa
     1801 cagtagaatg cctgggttct tgtggcaccg cacctatgct aatgatcgga gccgactacc
     1861 atgaaaacct aacattcgaa aaagtggata atctgattga gaaatgccga caatctggca
     1921 aaagatctag ttatcttgtt gataatatgc tcggataacg ctttaatatt attgtgaaat
     1981 gggaaaaaag cttttattaa aaaatattga tattccgggc attagtagct atgaggtata
     2041 tcgccgtgaa ggcggataca agtcagtaga aaaggttctt gccgaaaaaa acccggaagc
     2101 tgtagtggaa gaagtcaaga aatcaggtct tcgaggtcga ggtggtgcag gtttccctac
     2161 agggatgaaa tggagttttc tagctaaacc cgaaggtgtg ccccgctacc ttgtgtgcaa
     2221 tgcagatgag tctgagccgg gtactttcaa agacaggtac ttgatggagc acattccaca
     2281 tcttttggtc gaagggatga ttacttcaag ttatgcttta ggagccaata aatcattcat
     2341 ctacatccgt ggagaatatt tttatgtgct aagaatactt gaaaaagcta tcaaggaagc
     2401 ttatgataat ggctggttgg gtaaaaatat aaaaggaagt ggttttgacc atgatcttta
     2461 tgtccaaata ggtgcaggtg cttacatctg tggtgaggaa accgctttgc tcgaaagtct
     2521 tgaaggcaat cgtggaaatc ctcgtatcaa gcccccattt ccggcagtag ctggtctgta
     2581 cggttgccca acagtagtaa ataatgtaga aaccatagca gctgttgtac cgattatcaa
     2641 tgacggtggt gaggcttatg ctgccatcgg agttgaacga tctaccggca ccaaacttat
     2701 ttctgccagt gggcatatca aaaagcctgg cgtatatgaa atagagcttg gtttacctgt
     2761 agaagaattt attttttcgg atgaatattg tggaggcatc atcaacggac gaaaactgaa
     2821 agcagtagta gcaggcggtt cttcagtacc tgtattgcct gcagaattaa tcctcaaaac
     2881 agcagccggc acaccaagga tcatgactta tgaatcactt gcagacggtg ggtttgctac
     2941 cggaacaatg cttggttcgg gaggatttat tgtaatggat gaaaccgcaa gcgtacttag
     3001 gaatttacac acatttgcac gtttttatca tcatgaga
//
LOCUS       DAOQQY010000312         3016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_16867_length_3016_cov_6.470111, whole genome shotgun sequence.
ACCESSION   DAOQQY010000312 DAOQQY010000000
VERSION     DAOQQY010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16867_length_3016_cov_6.470111"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(427..1092)
                     /locus_tag="PLE23_11695"
     CDS             complement(427..1092)
                     /locus_tag="PLE23_11695"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HMX83972.1"
                     /translation="MVNREDNYRHKGLRKKLVEALLHKGIKDKAVLDAIGKLPRHFFL
                     DPSLDYQAYEDKALPIKADQTISQPYTVAFMTELLKIQKHDKVLELGTGSGYQAAVLS
                     LCGANVYTVERQETLFRESRKLLLSMNFNNIKFFYRDGTKGLPEFAPYDKIIVTAACN
                     EILQALLDQLAPGGMMVIPVGDDSQQMLTVEKSADGKIEKKIHGDFRFVPFLPGTASS
                     RER"
     gene            1256..2653
                     /locus_tag="PLE23_11700"
     CDS             1256..2653
                     /locus_tag="PLE23_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HMX83973.1"
                     /translation="MQTIRFGTDGWRAIIADDYTVANVLRVTDATARWLKNKGASQVV
                     IGHDCRFGGELFLQNAAAMFASYGIKSIVAKGFVSTPMVSLGVVKTKSDLGIVITASH
                     NPPSYNGYKLKSSFGGPSVPADIAGVEELIPDHEVRPSTTFEEAIAQGLISYIDLEKM
                     YIAHVEANFELDTIRSSGLQMAYDAMYGAGQNVIRHLFPDALLLHCEHNPSFMGQAPE
                     PIHRNLGELSNAIKSSGKFAFGLANDGDADRIGMYDDEGNFVDSHHLLLLLLLYLHKY
                     KGMTGEVIVTFSVTDKMKKMAELFGLPFTTKKIGFKYIAEFMLDHDVLVGGEESGGLA
                     VKGHIPERDGIWIGLTIMEFMAKTGKSIRELIREVYDLVGAFSTDRYDLHITEEQKQS
                     VIKACKENLYKTFGKYQVSRVEDLDGYKYYLDDERWVMIRPSGTEPVLRVYSQAPTHE
                     EAIEILEQTKKTILA"
     gene            complement(2640..>3016)
                     /locus_tag="PLE23_11705"
     CDS             complement(2640..>3016)
                     /locus_tag="PLE23_11705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMX83974.1"
                     /translation="VFVVEQNCPYLDADGKDENAYHFMLWKNDDLVGYFRLLPKGLSY
                     SDYVSIGRVVTAPLYRSQNLGRELMRQALIWCSILFPGEKIKISAQSHLENFYGSFGF
                     EPTGEYYLEDDIPHSAMVFMPR"
BASE COUNT          882 a          657 c          635 g          842 t
ORIGIN      
        1 atgtactgat gtactgatgt actgatgtac tgatgtactg atgtactgat gtactgatgt
       61 tttcatttta ttactcattt aaaagtgatt aaacattctg caagatacaa aagttttcat
      121 acccatgcaa gttttttaaa tatttttgaa aattattttc ggcattaata tcatacaagg
      181 tcatacaaca atataaaaaa gaataatatt ctgtttacga acaatctgtc ttcttctctc
      241 ttttctaccc caaacattaa cactgccttt gcctgtcatt tagctgagtg ccttgtatgt
      301 aaatggcaga attccagagt tgccgtccaa tcaaaaccta tcttacggaa gaaattatca
      361 atatcttatc caaaaatatc agaaaactaa aatcctcacc ggacatacat aaggcgaaag
      421 tgccattcac ctttcccggc tgcttgctgt accgggcaga aaaggaacga aacggaaatc
      481 gccatggatt ttcttttcta ttttaccgtc agcggatttc tctacggtga gcatttgctg
      541 tgaatcatct ccaacaggta taaccatcat gcctccggga gcgagctggt cgagtaatgc
      601 ttgcagtatt tcattacatg ctgctgttac gattatcttg tcgtaagggg caaattccgg
      661 aaggcctttt gtaccgtcgc gataaaagaa tttgatattg ttgaaattca tgctgagcag
      721 aagtttcctc gattcgcgaa acaaggtttc ctgtctttcg actgtataaa catttgcacc
      781 gcaaagggac aatactgctg cctgatatcc gctaccagta cccaattcga gtactttatc
      841 atgcttctga atctttaaca attcggtcat aaacgctacg gtgtaaggct gagagatggt
      901 ctggtcggcc ttgatgggca atgccttgtc ttcataagcc tgataatcta atgatgggtc
      961 gagaaaaaaa tgtctcggca gcttcccgat agcgtcaagt acggctttat ctttaatacc
     1021 tttgtgtaaa agtgcctcta ccagcttctt ccgtagccct ttgtgtcggt aattgtcctc
     1081 tctgttcacc ataattgctc aaaatattac acaaagctat gtttttgcta caacttaaca
     1141 gacaataaat gcgtactatt aagcatcaaa tagcattctt acagtatttc agtattacag
     1201 aatgcttgtt ttgttacaaa aaaatttaaa ctttgcactc tcaaaactta tacttatgca
     1261 gactatccgg ttcggaacag acggctggcg tgccattatc gctgacgatt atacagtggc
     1321 gaatgtactc cgagtaacag atgcaactgc ccgctggctc aaaaacaaag gtgcatcgca
     1381 agtagtcatc ggacacgact gcagattcgg cggagagctt tttctgcaga atgctgctgc
     1441 catgttcgcc tcctatggca tcaaatctat tgtcgctaaa ggttttgtgt ccacgcctat
     1501 ggtttcgctt ggtgtagtca agacaaaatc cgaccttggc atcgtcataa cggccagtca
     1561 caatccgcct tcatataacg gatataagtt aaaatcatcc tttggcggtc cgtcagtacc
     1621 ggctgatata gcaggtgttg aagaactgat acctgatcac gaggtaaggc cttctacaac
     1681 attcgaagaa gcaattgcac aaggtctcat cagttatatt gatcttgaaa aaatgtacat
     1741 agcgcacgtt gaggcaaatt tcgaacttga tacaatacgt tcttccggct tacaaatggc
     1801 ctacgacgcc atgtacggtg caggacagaa tgtcatcagg catttgttcc cggatgcctt
     1861 attgctccat tgtgagcaca atccgtcttt tatggggcag gcaccggagc cgattcacag
     1921 aaacctcgga gagctgagca acgccatcaa atcatcggga aaattcgctt tcggccttgc
     1981 caatgacggt gacgctgacc gtataggtat gtacgatgat gaaggaaatt ttgttgacag
     2041 ccatcacctg ctgctgttgc ttctgcttta tctacacaaa tacaaaggca tgaccggaga
     2101 agtcatcgtt actttctccg tcaccgacaa gatgaaaaaa atggcagaat tgttcggtct
     2161 gccttttacg accaaaaaaa tcggtttcaa atatattgca gaattcatgc ttgaccacga
     2221 tgtcctcgta ggtggcgaag aaagcggcgg attggcagta aaaggccaca ttcccgaacg
     2281 cgacggcatc tggattggtc ttactataat ggaattcatg gcaaaaaccg gaaaatcaat
     2341 cagggaactg attcgggaag tgtatgatct ggtcggagca ttctccacag acagatacga
     2401 cctgcacatt actgaagaac agaagcagtc ggtcatcaag gcgtgtaaag aaaatcttta
     2461 taagaccttc ggcaaatatc aggttagcag agttgaagac ctagacggct acaagtatta
     2521 tctcgacgac gaacgctggg tgatgatcag accgagcggt acagagccgg tattgcgggt
     2581 atattcgcag gcacctacac atgaagaggc gatagaaata ctcgagcaga ccaagaaaac
     2641 tatcttggca taaaaaccat agccgaatgc ggaatatcat cttccagata atattcaccg
     2701 gtaggttcaa agccgaatga cccatagaaa ttttcgaggt ggctctgggc agaaattttg
     2761 attttttcgc ctggaaacaa tatcgagcac caaataaggg cttggcgcat caattcacgt
     2821 ccgagattct gcgatctgta cagcggtgct gttactaccc gacctatgga aacatagtct
     2881 gagtaagaaa gtccttttgg gagtagccga aaatagccta caagatcatc atttttccat
     2941 aacataaaat gataggcatt ttcgtccttg ccgtcagcat caaggtaagg gcagttttgt
     3001 tctacgacaa acactt
//
LOCUS       DAOQQY010000313         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_16930_length_3010_cov_6.216920, whole genome shotgun sequence.
ACCESSION   DAOQQY010000313 DAOQQY010000000
VERSION     DAOQQY010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16930_length_3010_cov_6.216920"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLE23_11710"
     CDS             complement(<1..679)
                     /locus_tag="PLE23_11710"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HMX83975.1"
                     /translation="MKRHFLLINFLLSGIFFLTAQTKYTVSGYVRDSLTGETLIGVNI
                     YQQDQPANGTATNEYGFYSLTLSKAPLNLTISYIGYDKQTIRINSPTNQRLNISLYEG
                     LQISEVVVTAEQKDKNVNSTEMSTITLPVETIKKLPALFGEIDIIKSLQLLPGVQFGG
                     EGNSGFYIRGGGADQNLLLLDEANVYNGGHMLGFFSIFNSDAIKGTTLYKGGMPASYG
                     GRLSSVVD"
     gene            complement(751..1521)
                     /locus_tag="PLE23_11715"
     CDS             complement(751..1521)
                     /locus_tag="PLE23_11715"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMX83976.1"
                     /translation="MLWELSLSSSDAKSYVFGTMHAGSDEAYSFYNLAARYITNCTCF
                     ANEADLNEMNRGNAHLYLEGDQTLKTLIPEKKYIKIARIIKKSSGLELEALDRFKPII
                     IQNLMMESELMNNSRPPLDWQLKMLADQLGLRQTGVETVEDQIRIMNAIPLKIQLKTL
                     LKSIKRLKESKRGFKKLIRHYKMADLQKMSEQTKKSLGSLRKLMLYDRNVKMADWIFT
                     QSSIENTFVAIGAAHLGGNKGVLNLLRKKGFLLKAIHE"
     gene            complement(1596..2069)
                     /locus_tag="PLE23_11720"
     CDS             complement(1596..2069)
                     /locus_tag="PLE23_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HMX83977.1"
                     /translation="MSEKLDKTDLKILQILQENSKITNLELSKRIGLSPAPTLERVKK
                     LEQSGVIESYHANVNSQAIGLNVKTFALVSLAWHKENALNNFLSKIRAIREITECYII
                     TGEADFLIKIVTKDIPNYEKLLFKTLSQIEEIERLKTLMTLSTVKELKTLPFKYD"
     gene            complement(2457..>3010)
                     /locus_tag="PLE23_11725"
     CDS             complement(2457..>3010)
                     /locus_tag="PLE23_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HMX83978.1"
                     /translation="LKDNMAALELECDILVPAALENQITMENAHKIKAKIVAEAANGP
                     TTSEAADVLTKNGIMVIPDMYINAGGVTVSYFEWLKNLSHMRFGRMEKRFNSNTYDNL
                     LKTIEKLTGKTVGDKERMVLTRGAEEIDLVRSGLEETMVTSYHQIRETFQRKRKIEDL
                     RTAAFVTALQKISSDYENLGVFP"
BASE COUNT          816 a          578 c          581 g         1035 t
ORIGIN      
        1 tgtcaaccac cgaagaaagt ctgccgccgt agctggccgg cataccacct ttatacagag
       61 tagtcccttt tatggcatcc gaattgaaaa tcgaaaaaaa acctagcatg tgaccgccat
      121 tatagacgtt tgcttcatca agcagcaaaa ggttttggtc tgcccctccg cctcttatgt
      181 aaaatccgct gttgccttcg ccaccaaact gtacacccgg taagagttgc agcgatttga
      241 tgatatcgat ttcgccaaag agtgcgggca gtttcttgat tgtttctacc ggtagtgtaa
      301 tggtactcat ttcggtactg tttacatttt tatccttttg ctcagctgta acgaccactt
      361 cacttatctg caagccttca tatagtgaaa tgttcagacg ttggttggtg ggtgaattaa
      421 tccggatagt ttgtttatcg tagccaatat atgagatggt aagattcaag ggcgctttgc
      481 taagtgtaag tgaatagaac ccgtactcgt tggtagctgt gccatttgca ggctgatctt
      541 gctgataaat gttgacaccg ataagtgttt cgccggttaa cgaatctctc acatagccac
      601 tgacagtgta tttcgtctga gctgtcaaga aaaagattcc agaaagtaaa aagttgatga
      661 gtagaaaatg ccgcttcatt cagttctata tttttctaca aggttaaatg tgaattggtt
      721 caacggtcga atagtatgat aacgttctta ttattcatgg attgccttaa gtaagaatcc
      781 ttttttcctg agcaggttca gtacaccttt attcccaccg aggtgggccg cacctatggc
      841 tacaaatgta ttttctatcg atgattgtgt aaatatccag tctgccattt ttacatttcg
      901 gtcgtacagc atcagcttac gaaggctgcc aagcgatttt ttagtttgtt cgctcatttt
      961 ttgcaaatct gccatcttgt aatgtctgat aagtttttta aaacctcttt tcgattcttt
     1021 caatcttttg attgatttta agagtgtttt taactgtatc tttaacggaa tggcattcat
     1081 gattcgtatc tgatcctcga ctgtctctac acctgtttgt ctaaggccaa gctggtcggc
     1141 gagcattttc aactgccagt cgagtggagg tctagaattg ttcattagtt ccgattccat
     1201 catgaggttt tgaataataa taggtttaaa tctgtcgagc gcttcgagtt caagtccact
     1261 gcttttcttt atgatccggg ctatcttaat gtattttttc tccggaatga gtgttttcaa
     1321 tgtctggtct ccttcaaggt acaagtgagc atttccccgg ttcatttcat tgaggtcagc
     1381 ttcatttgca aaacaagtac agtttgtgat atacctggct gccagattat aaaatgaata
     1441 agcttcatcc gaaccagcgt gcatggtacc gaaaacataa gacttagcat cagatgatga
     1501 caagcttaac tcccacaaca aagaaccatt taatttggcc ataagtaaaa aagccgcata
     1561 ctcagatgcg gccttaggat ttatatttgt ttgaattaat cgtatttaaa aggcagtgtt
     1621 ttcagttctt tcaccgttga taatgtcatc aatgttttta gtctttcaat ctcttctatc
     1681 tgtgacaatg ttttgaacaa aagcttttcg tagttcggga tgtcctttgt tactattttg
     1741 ataaggaaat cagcttcacc tgtgatgatg tagcattctg tgatttctct gattgctcta
     1801 attttgctaa gaaagttgtt gagcgcattt tctttgtgcc atgcaagaga cacaagtgcg
     1861 aatgttttga cattaaggcc aatagcctga gaattgacgt ttgcgtggta actctcaatt
     1921 acacctgact gctcgagttt ttttactctt tcgagtgttg gtgccggtga taaaccaatt
     1981 ctttttgata attcgaggtt tgtaatttta ctattttctt gaaggatttg caaaattttt
     2041 aagtcggtct tatctaattt ttctgacatt ttgtgatgtt tatgatttag tttaattatt
     2101 atgtgaattt cgatgtaaaa ttaagcaatt gaaaaataaa atacaagatt tttgccaaaa
     2161 ttttaacaat tattcttaaa aacaaacaat ttagacttaa tataaaaaga atatttggtg
     2221 aagatatatc ataactgaca atacagaaca atccgaaaca taaattgttt gtaaacattt
     2281 caatttttta ccaaatattt tgcacaacta tcttaaaaca cccgattacg ggaaatgttt
     2341 tcctgaataa taagaaaatg agtatatcga ttacataaca tattcggcac aaaaaaagca
     2401 gtacccttat tcaggatact gctaatcatt cactaacaaa ataaaactta tctctattat
     2461 gggaacacgc cgagattctc gtaatcgctg ctgatttttt gcaaagctgt aacgaatgca
     2521 gcagttctca ggtcttctat ctttcgttta cgctggaatg tctcacggat ttggtggtat
     2581 gaagtcacca tagtttcttc aaggcctgag cgtacaaggt cgatttcttc agcaccgcgt
     2641 gtcagtacca ttctttcctt gtcacctaca gttttcccgg taagtttttc gatcgtttta
     2701 agcaagttgt cataggtatt gctattaaat cgtttttcca ttcggccgaa gcgcatgtgc
     2761 gaaagattct tgagccactc gaagtacgaa actgtaacac caccggcatt gatatacata
     2821 tccggaatga ccatgatacc atttttggta agcacatctg ctgcttcgga tgtagttggg
     2881 ccattggctg cctctgctac tatctttgct tttatcttat gtgcattttc catggttatc
     2941 tggttctcga gtgctgccgg aacgagtatg tcgcattcga gttcgagagc agccatgtta
     3001 tcttttagtg
//
LOCUS       DAOQQY010000314         2985 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_17174_length_2985_cov_5.739249, whole genome shotgun sequence.
ACCESSION   DAOQQY010000314 DAOQQY010000000
VERSION     DAOQQY010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17174_length_2985_cov_5.739249"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PLE23_11730"
     CDS             complement(<1..607)
                     /locus_tag="PLE23_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX83979.1"
                     /translation="METGITKSKVILEIKNLNKSFGSNTVLKNFNLTLHEGENIAVLG
                     KSGSGKSVLIKCIIGLLMPEKGEIWAFGDKNIPSLGLEELDIIRSKIGFLFQSNALYD
                     SMTVRQNILFPLRRHWIEMDKDHEDLLVHETLQNVGLAHTAEMMPSELSGGMRKRIAL
                     ARTLVLKPKIMLYDEPTTGLDPITSREISRLIVELQEKYKTS"
     gene            complement(620..1393)
                     /locus_tag="PLE23_11735"
     CDS             complement(620..1393)
                     /locus_tag="PLE23_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX83980.1"
                     /translation="MIQLPNRWGEIVEETGKLSQFAGNFFVQLFRPRFEGKEFLRQCY
                     TIGYLSLGLVGLTGFIMGLVLTLQVRPIMLQYGVEAKIPAMVSLSIFREIGPVITALI
                     FSGKIGSSIGAELGSMKVTEQIDAMEVSGTNPYKFLVVTRVLACTLMLPVLVLLADLI
                     GLYGSFIGINIHGSTSFALFFKQVFEAMSFGDVLPAFFKTFVFGYAVGIIGCFKGFNS
                     TKGTEGVGKSANSAVVLSALAVFIIDLIAVQVTDLFNFT"
     gene            complement(1539..1904)
                     /locus_tag="PLE23_11740"
     CDS             complement(1539..1904)
                     /locus_tag="PLE23_11740"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HMX83981.1"
                     /translation="MLCTITSKAEVADPIWDAVSDAFRNGNIRSVSGYFGKMVDLTIV
                     NTENTETKDQAISSLETFLSKNKPTGFTQVHQGTSRGKESYYCIGELNTQNGKFRVYV
                     YFKTVSTLTQIQEVRIDKV"
     gene            complement(2013..>2985)
                     /locus_tag="PLE23_11745"
     CDS             complement(2013..>2985)
                     /locus_tag="PLE23_11745"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMX83982.1"
                     /translation="AAQELCLKNGITSFHDAGVSVKDFANYVRYAEQGQMTVRMYLML
                     LDSIKNIESIEDRLPMKGHYNGFLTVRAIKQFIDGALGSHGAWLLKPYTDKPALTGQN
                     VLDLQSLSDFAEYALNHHLQLCVHAIGDRGNREVLNIYESAIMSQRDHDNLRWRIEHA
                     QHVSPEDLGRFGKLGIIASMQAVHCTSDAPYVTKRLGEERARTESYMWRSLIDSGAHL
                     ANGTDTPVEKENPFDCIYSAVTRKRKDTGLEFYPEQKMTREEALLSYTLWNAYAAFEE
                     DLKGSIKVGKLADLIVTDRDLLKCPESKIPDTKVVMTILNGKIVYQQ"
BASE COUNT          859 a          656 c          545 g          925 t
ORIGIN      
        1 atgatgtttt gtatttttcc tgcaattcta caattagtct acttatttca cgtgaagtaa
       61 tggggtcgag cccggttgta ggttcatcat agagcatgat ttttggcttc agtacgagtg
      121 tgcgggctaa tgcaattcgc ttgcgcatcc cacctgacag ttccgatggc atcatctctg
      181 ctgtatgagc cagccctaca ttctgaagtg tctcatgtac aagtaaatct tcatgatctt
      241 tatccatttc aatccaatgc ctcctgagtg gaaagagtat attttgtctg actgtcatag
      301 aatcgtacaa cgcattgctt tggaaaagga aaccgatctt tgatctgata atatcgagtt
      361 cttccaggcc aagggatggg atattcttgt caccaaatgc ccatatttca cctttttcgg
      421 gcattagtaa acctataata cattttataa gaacggattt ccctgaaccc gatttgccca
      481 aaacagctat gttttcacct tcatggagtg taagattgaa atttttcaaa acagtatttg
      541 agccaaaact cttattcaga tttttgattt ctagtattac tttcgacttg gtaatgcctg
      601 tttccaattt caactgaatt tatgtaaaat tgaataagtc agtaacttgt actgcaatta
      661 ggtcaatgat aaatacagca agcgcactca aaaccactgc cgaattggct gatttgccta
      721 caccttcggt acctttggtc gaattgaacc ctttaaaaca accaataata cctactgcgt
      781 atccaaatac aaatgttttg aagaatgcgg gtaaaacatc accgaatgac attgcctcaa
      841 atacctgttt gaagaatagg gcaaaacttg tacttccatg tatatttata ccaataaagg
      901 aaccatataa accgataaga tcggctaata ataccagcac aggtaacatt aaggtacatg
      961 ccaatactct ggtcactacc agaaatttgt aaggattagt acccgaaact tccattgcat
     1021 caatctgttc agtcactttc atgctaccca gttcggctcc aatactcgaa ccaattttgc
     1081 ctgaaaaaat caaggcggtt attacaggac caatctcgcg gaaaatggat aaggaaacca
     1141 tagccggaat ctttgcttcg acaccgtatt gaagcattat cggtcgtact tgcagggtta
     1201 gtaccaaacc catgataaac ccggttagtc ctaccaaacc caacgataaa tatccgatag
     1261 tataacattg acgcagaaat tctttgcctt caaatcgcgg tctgaacaac tgtacaaaaa
     1321 aattgcctgc aaattggctg agcttgccag tttcttcaac gatctctccc catctgtttg
     1381 gtaactgtat caaaccttat attttgtaac gtataaagtt actcacaaat cacgtactat
     1441 ccaaggattg aacctaatta aaaaaaacta cttgaaggcg gataataatc ggcaaccaat
     1501 ttgtgtgtga accggcacct ctcagacaca aaacatgctt agaccttgtc tattctcact
     1561 tcttgtatct gggtcaatgt tgaaacggtc ttaaaataca cataaaccct gaatttcccg
     1621 ttttgagtgt taagttctcc tatacaataa taagattcct tacctcttga tgtaccctga
     1681 tgtacttgag taaatcctgt aggtttgttt ttactgagaa atgtctctaa tgaagaaata
     1741 gcctgatctt tcgtttctgt attttcagta ttaacaatgg tcagatccac cattttaccg
     1801 aaataaccgg atacgcttcg aatgttccca ttgcgaaagg catctgacac tgcatcccaa
     1861 ataggatcgg ctacttcagc tttcgaggtg atagtacaaa gcataagaat catgaataaa
     1921 aacctactca tcattgtggt aaataacttt atatattctg acgcaatact aagctgaaaa
     1981 gttcactttt taaaaacatt aacatttgat tattattgct ggtaaacaat ctttccattg
     2041 agaatggtca tcactacttt tgtatcagga atcttggatt ccgggcattt gagcaaatct
     2101 ctgtcagtaa ctatcaggtc tgccagtttt cctactttga tggaaccctt cagatcttct
     2161 tcaaatgcgg cataggcatt ccacaaagtg tagctcagca gggcctcctc acgggtcatt
     2221 ttttgttctg ggtagaattc gagccctgta tcctttcttt ttcgggtaac tgccgaatat
     2281 atacaatcaa atggattctc cttttctacg ggtgtatcgg taccgtttgc caaatgagcg
     2341 ccactgtcta tgagcgaacg ccacatgtat gactctgtcc gcgcacgttc ttcaccaagc
     2401 cttttggtaa cataaggtgc atctgatgta cagtggacag cttgcattga cgcaataatc
     2461 ccaagcttac cgaaacgccc taaatcctcg gggctgacat gctgagcgtg ttctatacgc
     2521 caccgtagat tgtcatggtc tcgctgactc ataatggccg actcgtatat attcaacact
     2581 tctctgttac ctcggtcgcc aatggcgtgt acacataatt gtaaatgatg gttaagcgca
     2641 tattcggcaa aatcagataa agactgcaag tcaagaacat tctgccctgt aagagccggt
     2701 ttgtcggtat atggtttgag tagccatgca ccgtgtgagc cgagtgctcc gtctatgaat
     2761 tgcttgatag cacgtactgt cagaaatccg ttgtaatgcc ctttcatcgg tagtctgtct
     2821 tcgatgcttt ctatgttttt gattgaatca agtaacatca gatacatccg tactgtcatc
     2881 tgtccttgtt ctgcatacct tacataattg gcaaaatctt ttactgacac tccggcatca
     2941 tgaaaacttg ttattccgtt tttaagacat agttcctgag ctgct
//
LOCUS       DAOQQY010000315         2949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_17500_length_2949_cov_4.825847, whole genome shotgun sequence.
ACCESSION   DAOQQY010000315 DAOQQY010000000
VERSION     DAOQQY010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17500_length_2949_cov_4.825847"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLE23_11750"
     CDS             complement(<1..945)
                     /locus_tag="PLE23_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HMX83983.1"
                     /translation="MFKYLERIKSVVKGKLEVLGLFSWMRTTSFPGAFGVTLYDLYLF
                     VRKESRQFDISVRANSMSFNFFLSLFPLLLVMFTIIPFLPLNDHFFGTLRAAIMEVMP
                     GKTGKSLYSFIKDLATKPQLNLLSVSSILVIYFASNGVMAMINGFEKSYPTTFEEWPG
                     WYKRLRAIGLTFLLAIILLASIILIILGNVILKWFYSLTGIIPSSFQSGSFHLLRWLV
                     IIALYYTGISSIYRFAVSARKRFAFFTPGATLATVLSLLTSYLFSFYIDNFSSYNKLY
                     GSIGTVIALMLWIQFNAFILIIGFELNASIAITRDMKIM"
     gene            complement(938..1351)
                     /locus_tag="PLE23_11755"
     CDS             complement(938..1351)
                     /locus_tag="PLE23_11755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMX83984.1"
                     /translation="MFVHETQIRVRYSETDRMNYVYYGNYPQYFEVGRVEAMRSIGIS
                     YKDLEENHGVMMPVKSMSIKYLRPVFYDELITVKTIVSALPERSINFHVEIFNEKGEI
                     TTGGTVSLAFVNIETKESIPCPEILIKQLAKYFDV"
     gene            complement(1355..2626)
                     /locus_tag="PLE23_11760"
     CDS             complement(1355..2626)
                     /locus_tag="PLE23_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HMX83985.1"
                     /translation="MSKSSKVSVSSEVDRLMKVIVHRPDEGIARITPKRAGELLFDDI
                     VHLPNMQDEHDVFTDVLKYFLGEDNVLETGQLLYEALVADEETKFEIIEMIINYEELP
                     ESYIHLLKKLPNDQLADALITGHLVSEDLILFDPIPNFIFTRDIAVVVNDHVIITKAA
                     KMARQRENFLTRFIFWSHPIFRKLREEGKIINLNHVDEFPPSRKGETVSIEGGDVMVI
                     NKDYILIGCSERTTEYSIHSLRNKLFEKGLVNNVVQINIPADRAYMHIDTIFTQINHD
                     HIVAFKPIIEDGLGSYVDVYRKDGSTVTYDSVKEFVEAEINPDMKFIWSGNGESPYQE
                     REQWTDGCNLVALKPGVAITYDRNPVTEEAFKEFGYTIMAAKDFLDLVKRENPAPDSF
                     NNLIITIPSNELSRARGGTHCMTCPVHREKS"
BASE COUNT          923 a          609 c          522 g          895 t
ORIGIN      
        1 cattatcttc atgtcgcgcg ttatggctat gcttgcattg agctcaaaac caatgatgag
       61 aataaatgca ttaaactgta tccaaagcat taaggcgata accgtaccaa tagagccgta
      121 aagtttattg taagagctaa aattatcaat ataaaacgag aaaaggtatg atgtgagcaa
      181 cgacaatact gtagctaatg tagcgcccgg tgtaaaaaat gcaaaccgct tacgtgccga
      241 tactgcaaac ctgtaaatgg acgaaatgcc cgtataatat agtgcgatga tgacaagcca
      301 cctcaacaaa tgaaacgagc ccgactgaaa cgaactcggg ataataccgg taagtgaata
      361 aaaccacttg agtatcacat tgcctaaaat aattaaaatg atggaagcaa gcaaaatgat
      421 agccagcaag aatgttaagc caatcgccct tagtcgtttg taccatccgg gccattcttc
      481 aaaagttgtc gggtatgatt tttcaaagcc atttatcatc gccatcacac cattggatgc
      541 aaaataaatc accagtatag atgataccga aagcaagttg agctgtggct tagtggccaa
      601 gtctttgatg aacgaataaa gagattttcc tgttttacca ggcattactt ccataatagc
      661 agcgcgaagt gttccaaaaa aatgatcgtt caaaggaagg aacggaataa tcgtaaacat
      721 taccaacaac aaagggaaaa gcgataaaaa gaagttaaat gacatcgaat ttgcccgtac
      781 actgatgtca aattgtcgcg actccttcct tacaaacaaa taaagatcat ataaagtaac
      841 accaaaagct ccgggaaatg atgtggtacg catccacgaa aacaaaccaa gaacttcgag
      901 ttttcctttg acaacactct ttattcgctc caaatattta aacatcaaaa tactttgcca
      961 attgtttgat caatatttca ggacaaggga tcgactcttt ggtttcaata ttcacgaatg
     1021 caaggctcac tgtaccaccg gtcgtaatct ctcctttttc attaaatatc tctacatgga
     1081 aattgatgct tctttccggt aaagccgaca ctattgtttt gacagtaata agttcgtcat
     1141 aaaatacagg tctgaggtat ttgatactca tagacttaac cggcatcatc acaccatggt
     1201 tctcttcaag atctttgtac gaaataccaa tggaacgcat agcttctacc ctacccactt
     1261 caaagtactg tggataattg ccatagtaaa cataattcat ccggtcagtt tcgctatacc
     1321 tgactcttat ttgggtctcg tgtacaaaca tgaattaact cttttctctg tgtacagggc
     1381 atgtcataca gtgggtaccg cctctggcac gtgacaattc gtttgaaggt atggtaatga
     1441 taagattatt gaatgaatcc ggtgccggat tttctctttt tactaagtca agaaaatctt
     1501 tagctgccat aatagtataa ccaaattctt taaatgcctc ctcggtaacc ggattcctat
     1561 cataggtgat ggctacaccg ggcttgagtg ctacaagatt gcagccgtct gtccattgtt
     1621 cgcgttcttg atatggcgac tcaccatttc ctgaccagat gaacttcata tcaggattta
     1681 tttcagcttc aacaaattct tttaccgaat cgtaggtgac tgtactacca tcttttctat
     1741 atacatctac ataactaccc aacccgtctt cgataatggg tttgaaagcc acaatatggt
     1801 catgattaat ctgagtaaag atcgtatcaa tgtgcatata agctctgtca gcaggaatgt
     1861 tgatctggac tacattgttc acgagccctt tttcaaaaag tttattgcgt aggctgtgga
     1921 tagaatactc tgtggtacgt tcactgcagc ctatgagtat ataatcctta ttgataacca
     1981 ttacatcgcc cccctcgatt gatacagttt ctcctttacg agatggcgga aattcatcaa
     2041 catggttcag attaattatt ttgccttctt cgcgaagttt tcgaaaaata ggatgcgacc
     2101 agaaaataaa gcgggtgagg aaattttccc tttgccttgc cattttagca gcctttgtga
     2161 tgataacgtg gtcattcact acgacagcaa tatctcttgt aaaaatgaag ttaggtatcg
     2221 ggtcaaaaag tatcagatct tcgctcacaa ggtgtccggt gattaaggca tctgccaact
     2281 ggtcattcgg caattttttt agcagatgta tgtaagattc aggcaactct tcgtagttaa
     2341 taatcatttc gatgatttcg aattttgttt cttcatcggc tactaatgct tcgtataaca
     2401 actgcccggt ttcaagcaca ttgtcctctc cgagaaaata tttcaaaaca tccgtaaaca
     2461 catcgtgctc gtcctgcata ttgggcaaat ggacaatatc gtcaaaaagc agttcgcctg
     2521 ccctcttggg tgttatgcgg gcaatacctt catccggcct gtgtacaatc actttcatca
     2581 acctgtccac ctccgacgaa acagatactt tcgaactctt actcatatat ttacttcttt
     2641 ttttaacaaa actttatcta tcatcttata attgaacaac ttgtccctta tatcaattcc
     2701 ttctatttaa ctcatctcac cacatggctt tagctgcata tatttcaacc gaaaaatgat
     2761 gatgcaaatt tcgtgatttt gtttgttatt tcaaaaatcc acgacttgaa gaagcaattt
     2821 tataaaaaat aatttgaatt tactaaattt acggctgttt taatacaatt tgacgattga
     2881 aaatacatat ttgtcgaatt gcaggaactt gtttgaataa aaattaatta tttactgtgt
     2941 acaaattat
//
LOCUS       DAOQQY010000316         2945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_17538_length_2945_cov_4.929066, whole genome shotgun sequence.
ACCESSION   DAOQQY010000316 DAOQQY010000000
VERSION     DAOQQY010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17538_length_2945_cov_4.929066"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..807)
                     /locus_tag="PLE23_11765"
     CDS             complement(112..807)
                     /locus_tag="PLE23_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83986.1"
                     /translation="MTKNLSSVFSILLLLTIFSPHSFAQSKRKIACQLAHDLKQGTLI
                     VVLKTDAKKLAYLEKFSKQGSDREMARYAKMLRRTLAERDTTWKYTTLGFSTLYQFSA
                     ATFLMDTSLGTFLKNPADCTFYDAALKPITHIPQGRLFVCKYGHTFGDQSTNLQNAWY
                     VMDSHLQRLPKPFPEMVRFSVFRPAFTLRLPPDFMQQNGISKTTKHHKPLRYAMKLNA
                     DLIKYYTKKGDCE"
     gene            941..1948
                     /locus_tag="PLE23_11770"
     CDS             941..1948
                     /locus_tag="PLE23_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMX83987.1"
                     /translation="MVEQFIHTDIERLNGEILVRSQFIDKCLSEISGQIVGQHYLLER
                     LMIALLSDGHILLEGNPGLAKTLAIKSLAAAFHVSFQRLQFTPDLLPGDITGTLIYNP
                     GKSTFEVKKGPIFANFILADEINRAPAKVQSALLEAMAERQVTLGENTYRLPVPFLVM
                     ATQNPIEQEGTYPLPEAQVDRFMMKVKISYPQFEEERKIIRSHTGTVPAPEVKAVVSL
                     EELNDARRLVKQIYLDEKIEKYILDIVFATRTPDVYRLKDIRSMISFGASPRASINLA
                     LAAKAHAFIHHRAYVTPDDVKAVAADILRHRIGITFEAEAENITSDEIIHKILGVIQV
                     P"
     gene            1949..>2945
                     /locus_tag="PLE23_11775"
     CDS             1949..>2945
                     /locus_tag="PLE23_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83988.1"
                     /translation="MKRILYIALLVWLGNICNATGQAASKDELEALFDTPDKIQWTRN
                     YKGLINNIESVEISLASDGRNCRGYVLFPKSQTTIELRGILSGSRIELRELNDEGLIT
                     AYLKGSLTPGKIEADWENYNHTIGCKYVLYSESEAAKLSPASQKKWIKWYSGEVAHEK
                     LRIILFQKEPEKLTGFCFNDTGTRMYNVNGMLNPDKSIFLNFLLDETEVARFDGKVKS
                     GAIIKGTTTGIGVTSEPLQLELENELNLEETSYQNFRTAIEILTPKLRKGNFEKYILE
                     KIEPYIQEVNSEIVENLSESHVPQPSERLSQRAYIYPVIRFVSKEIISGHILYTNT"
BASE COUNT          868 a          578 c          771 g          728 t
ORIGIN      
        1 cttttcactt ataactttta atttttaatt tttaattttt aattgaatgc gtgtaaggga
       61 tagaagccgg cacgaagtaa ggcggatagc ccgacccgac aggggcacac cctactcaca
      121 gtctcctttc ttggtataat actttatgag gtcggcattg agcttcatgg cgtagcgaag
      181 aggtttgtgg tgtttggtag tcttactgat accgttttgc tgcataaagt cgggtgggag
      241 cctgagggtg aaagccggtc tgaaaacaga gaagcgcacc atttcgggaa atggttttgg
      301 gaggcgctgc aagtgagaat ccatcacgta ccaggcattc tgaagattgg ttgactggtc
      361 gccgaaggta tggccgtact tgcagacgaa cagccggccc tgtggtatgt gggtaatggg
      421 cttgagagct gcgtcgtaga aggtgcagtc ggcagggttt ttgagaaatg tgccaagcga
      481 agtatccatc aggaaggtgg cggctgaaaa ctggtataat gtactgaaac ccagtgtggt
      541 atatttccaa gtagtgtcgc gttcggcaag tgtgcgacgg agcattttgg cgtagcgtgc
      601 catctcgcgg tcagagcctt gtttggaaaa tttttcgagg taggcaagtt ttttggcgtc
      661 ggtcttcagt acgactatca gggtaccttg tttgaggtcg tgtgcgagct ggcaggcgat
      721 tttccttttt gactgtgcaa aactatgtgg agagaatatt gttaacaaga gaaggattga
      781 aaagacggaa gagagatttt ttgtcattgg ttcgaacttt aatattttat taaggtaaaa
      841 ttatatgcaa cattcagaac tttgtgcgtt aatcataaac attttctgaa gtaggtggat
      901 aaaagtttat ttttacatac caataacgag ctaaacagag atggtagaac aattcataca
      961 cacagacatt gagcggctaa acggggagat actcgtgagg agccagttta tagacaagtg
     1021 tttgtcggag atatcggggc aaatagtagg gcagcattat cttcttgagc ggttgatgat
     1081 agccttgctg tcggacggac acatcttgtt ggagggaaat ccggggcttg ccaagacgct
     1141 tgccatcaaa tcgttggcgg cggcatttca tgtgagtttt cagcggctac agtttacgcc
     1201 ggacctattg ccgggtgata taacaggtac actgatatac aatccgggga aatcgacatt
     1261 tgaggtaaag aagggtccga tatttgcaaa tttcatactg gcagatgaga taaaccgtgc
     1321 tccggcgaag gtacagtcgg cattgttgga ggctatggcc gagcgtcagg tgacattagg
     1381 agaaaacact taccgcctgc cggtgccgtt tctggtgatg gccacccaaa atccgataga
     1441 acaggaaggt acgtatccgc taccggaggc acaggtggac cgtttcatga tgaaagtaaa
     1501 gataagttac ccacagtttg aggaggaacg caaaatcatc cgttcacata cgggtacggt
     1561 gcccgcacct gaagtgaagg ctgtagtgag cctcgaagag ctcaatgatg cgcgccgtct
     1621 ggtgaagcaa atataccttg acgaaaagat agagaaatac atattggaca tagtcttcgc
     1681 tactcgcaca ccagatgtgt acagactgaa agacatacgt tctatgatta gttttggcgc
     1741 ttcgccaagg gcaagtatca accttgcact ggctgctaag gctcatgcgt ttattcatca
     1801 tcgggcttat gtaacacccg atgacgtgaa ggcagtggct gccgatatcc tgcgacaccg
     1861 catcggcatc acattcgaag cagaagccga aaacataaca agtgacgaaa tcatccataa
     1921 aatactgggc gtaatacagg taccttaaat gaaaagaata ctctatatag cattacttgt
     1981 atggctggga aatatatgca acgctaccgg gcaagccgcc tcgaaagacg aactcgaagc
     2041 attgtttgac actcccgata aaatacagtg gacaagaaac tacaaagggc tgataaacaa
     2101 catcgaatct gtagaaatat cactggcgag tgacggaaga aactgcaggg gatatgtatt
     2161 gttccccaag tcgcagacca ctatcgagct ccgtggcata ctgagcggca gccggataga
     2221 actgagagag ctaaacgatg aaggcctgat aacggcttat ctgaagggat cgctcacacc
     2281 aggcaagata gaagctgatt gggagaatta taaccatact ataggctgta agtatgtact
     2341 ttactctgag agtgaagcag caaaactgag tccggcatca cagaaaaaat ggataaaatg
     2401 gtattcgggc gaagtagccc atgaaaagct acggattata ctgttccaaa aagaacccga
     2461 aaagctgaca gggttctgct tcaatgacac aggtacaagg atgtacaatg tcaatgggat
     2521 gctgaacccc gataagagta tttttttgaa ttttctgctg gatgaaacag aagttgcaag
     2581 gtttgacggt aaggtcaaga gtggtgccat catcaaaggt actaccaccg gtatcggtgt
     2641 cacaagtgaa ccattgcaac tggagctcga aaatgaacta aacctggagg agacatcgta
     2701 ccagaatttc cgaactgcca tcgaaatctt aacgccaaag ctcagaaagg ggaatttcga
     2761 aaaatacatt cttgagaaga ttgagccgta tatacaggag gtaaactccg aaatcgtaga
     2821 gaatctgtct gaaagtcatg taccgcagcc atctgagcgt ctatcgcagc gtgcttatat
     2881 atatccggtg ataaggtttg taagcaagga gatcatcagc ggacatatac tatataccaa
     2941 cacct
//
LOCUS       DAOQQY010000317         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_17555_length_2944_cov_4.435099, whole genome shotgun sequence.
ACCESSION   DAOQQY010000317 DAOQQY010000000
VERSION     DAOQQY010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17555_length_2944_cov_4.435099"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PLE23_11780"
     CDS             complement(<1..884)
                     /locus_tag="PLE23_11780"
                     /inference="COORDINATES: protein motif:HMM:NF015286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase beta chain"
                     /protein_id="HMX83989.1"
                     /translation="MESISIFEMIKIGIGPSSSHTMGPWKAAQMFVDELGDDLEKLVT
                     IYVHLYGSLAKTGKGHGTDVAVLIGLTGADYTQIDTREVMPLFEKIREEKKMLVKGKY
                     EIPFIYDDHLIFDYQHSLPYHPNGMTFIATLENGVQISRSYYSVGGGFVATEYSNSVV
                     ENAIRTPYPCHVAASIPEYCKQLSLRVSDLVYVNEEAWRPRDEINAGIKKIWKEIKDC
                     IFRGVYTEGILPGGLEVKRRAPDINRKLLGPHVKPKTAEAWIELVKACQPSFNNINKW
                     VSCFALAVNEENAAFGRI"
     gene            complement(958..1680)
                     /locus_tag="PLE23_11785"
     CDS             complement(958..1680)
                     /locus_tag="PLE23_11785"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HMX83990.1"
                     /translation="MVLSGQKEVKLVSYETTVERRNAGKVPKVQKAISALTPDENLFV
                     LLRKVRKSIADKEGKLHYHVFGDATLKQMAAIVPLTKEELLRVTGVGEYKAQKYGDFF
                     LDVISDFVKGNPELKNQSAQTFHATPEPEKKAMKPLVGKEQKNQILDLFRQKYTIEQI
                     AAEFARSKETILKYLLEFYRNNPESLDVYQLVTHEQVELVKAAKNALDQTDRLKPYYD
                     YFNGQLDYDVIRVSLAVLQMNQ"
     gene            complement(1696..2883)
                     /locus_tag="PLE23_11790"
                     /pseudo
     CDS             complement(1696..2883)
                     /locus_tag="PLE23_11790"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
BASE COUNT          746 a          661 c          535 g         1002 t
ORIGIN      
        1 atgattcttc cgaatgcagc attttcttcg ttgacggcaa gtgcaaagca ggatacccac
       61 ttattgatat tgttaaaact cggctggcat gcctttacta gctcgatcca ggcttcggca
      121 gttttgggtt ttacgtgcgg tccaagcaat tttcggttta tatccggtgc tcgccttttc
      181 acttctaggc caccgggtag aatgccttct gtataaactc ctctgaaaat acaatctttt
      241 atttctttcc aaattttttt tatacctgca ttaatttcat ctcgaggacg ccatgcctcc
      301 tcatttacat aaaccaagtc agacacacgc aagctgagtt gtttgcagta ttctggtatt
      361 gaagcagcta catggcacgg gtaaggtgtc cgtatagcat tttctaccac cgaattggaa
      421 tattcggtag caacaaaacc accgccaact gaataatagc ttctgctaat ctgcactccg
      481 ttttcaagcg tagcaataaa agtcattcca ttcgggtggt atggcaatga atgttggtaa
      541 tcgaatatca aatgatcatc gtaaatgaat ggaatttcat atttcccttt gacaagcatt
      601 tttttctcct cccgaatttt ttcgaataat ggcatcacct cacgagtatc aatttgggta
      661 taatctgccc ctgtaagccc aatcaataca gctacatctg tgccgtgtcc tttacccgtt
      721 ttggctaatg aaccataaag atgcacatat atggttacaa gtttttcaag gtcatcaccc
      781 aattcatcta cgaacatctg ggcagctttc catggaccca tagtatggga agaggaaggt
      841 ccaattccga tttttatcat ttcaaatata ctgatggatt ccattattct gatttttaat
      901 tgtaatgaaa ttgagattcc aaaggtacaa aaaatgagga aactttttcc ttacagctta
      961 ttgattcatc tgtaacacag caagcgaaac ccgaataaca tcataatcaa gctgtccgtt
     1021 aaaataatcg taatacggtt taagccgatc agtctggtcg agtgcgtttt ttgcagcttt
     1081 cacaagttca acttgttcat gtgttactag ttgataaaca tctaaacttt caggattgtt
     1141 acggtaaaat tcgagcaaat atttcagtat ggtttccttt gagcgtgcga actcagctgc
     1201 tatttgttca atcgtatatt tttgtctgaa caaatctaat atctggttct tttgctcttt
     1261 tcctaccaat ggcttcattg cttttttctc aggttcaggt gtcgcatgaa atgtttgggc
     1321 actctgattc ttcaattcag gatttccttt aacaaaatct gatattacat ccaaaaagaa
     1381 atcgccatac ttctgtgcct tgtactctcc aacacccgtc acccggagca actcttcttt
     1441 cgttaaaggc acaatggctg ccatctgttt gagtgttgca tctccaaaca catggtaatg
     1501 cagctttcct tctttatctg caatgctttt tcttactttc ctcagcaata caaacaagtt
     1561 ttcatcaggt gttagggcag atattgcttt ttgaactttt ggcaccttgc cagcattgcg
     1621 cctttcaact gtagtctcgt aactgaccaa ttttacttcc ttttgcccgg ataagaccat
     1681 ccgtgaagct tcattgagtt tcagcttact gtggtctttg taatcaatct caatgattcc
     1741 ttgctgaatg aattgctgga tatacatagc ccaattggaa gcagtaattt gccttcctgc
     1801 gccgaaggtt tataatgtgt catagccatt ttctttaatc gacggcgtaa atgtgccttt
     1861 cagaatgtca atggtggtag tcatgccagt ctgttcgttg gtacgggtaa tggccgacaa
     1921 agccatttgg gcagggattg tgccattttc gtactttggc ggattcagac atacgtcaca
     1981 atttccgcaa tctgctgctg tctgctcact aaaatatgca agaagtactt ttctccggca
     2041 tatttgcgac tcggcatatt cctgaattcg ctttaattta tcttcctgaa ttttacggta
     2101 ttcttcattc tcaatctgat tgatgaaatg catctgagta gccacatctc cgtatccata
     2161 gaacaaaatt gtctctgagg gtagcccgtc tctgcctgcc cgacctatct cctgataata
     2221 tccttcgagg tttttaggca aattgtaatg cattacaaat ctcacatccg gcttgtcgat
     2281 gcccattcca aatgctatgg tcgctacaat aatttttaac ttaccttcta taaatgccgc
     2341 ttgcacccta ctcctttcca acggatcaag accggcatga taacatgctg cgtcgtaccc
     2401 ttccgagacc aacttttctg tcaatttctc ggtttgtttt ctgcttgagc aatatattat
     2461 accactttga tctgcatgtt tcccaatcat cttgagtatt tgattgtacc tgttcgttgc
     2521 cggtactacc gctagtgaaa gattgggtct gtcaaatgaa ctgatgaaca cttgttcatc
     2581 ccacataccg agcaattcgg ctatatcact tcgtaccgct ctatctgctg tagctgtaag
     2641 tgcaattatc ggtatttccg ggtatgtttc tctaatcctt ttcaattgcg catattccgg
     2701 cctgaaatca tgcccccatt gagatgtgca atgcgcctca tcaatcgcta tcagtttgat
     2761 gttataatat ttcaactgcc caaagatgct tgagcgtagc aatttttcag gtgaaatata
     2821 gagaatttta agttgattgc ttgttatctt ttggttaatc atcctttctt ctatatctga
     2881 caaggacgag tttaggtatt ctgcttctat tccgttcgac tttaatgcgt caacctggtc
     2941 tttc
//
LOCUS       DAOQQY010000318         2912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_17871_length_2912_cov_7.112706, whole genome shotgun sequence.
ACCESSION   DAOQQY010000318 DAOQQY010000000
VERSION     DAOQQY010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17871_length_2912_cov_7.112706"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="era"
                     /locus_tag="PLE23_11795"
     CDS             complement(<1..886)
                     /gene="era"
                     /locus_tag="PLE23_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HMX83991.1"
                     /translation="MELTETKEGFKSGFVNIIGKPNVGKSTLINALIGERMSIISPKP
                     QTTRHRILGILTTDNYQMVFSDTPGFIEKPAYKMHKAMNKFVHSSFEDADVFLFVTSP
                     DDRYGEEDPLIDLMNSVSEPVILVINKIDLSNKEELELLIEKWKTQIPVKEVVLISAL
                     NNFNINALLEHIEVFLPDGPMYFPADQLTDRLERFFVTEIIREKVFLQYRDEIPYTTE
                     VVIDDYKETTTKDGQPLARIRAQLLVERESQKAIILGKNGGGIKKLATTSRIDIENFI
                     QCKVFLEISVKVAPKWRNS"
     gene            complement(909..1493)
                     /locus_tag="PLE23_11800"
     CDS             complement(909..1493)
                     /locus_tag="PLE23_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83992.1"
                     /translation="MHDIEPYFAWRDDYAAEEDEHSPFYGREYDEFHYHNKIYNYYIH
                     PQWDEFGSLTLYTKILYADYDEGFAILEFIGEWNDCLYNDIKFLKEEVIDVLFKHGIN
                     RFIFICENVLNFHGSDDCYYEAIYEDISEEMGWMAFVNTLPHVEEEMVNTHIQYYAYL
                     GANLSDVNWRRYKPKQILQLIEQYLNTHRNRLQY"
     gene            1497..2111
                     /locus_tag="PLE23_11805"
     CDS             1497..2111
                     /locus_tag="PLE23_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMX83993.1"
                     /translation="MFINLIAIAYYFLKNLYHITKINRLFELMENIRRFVFTGPESTG
                     KTSLSIGIADYFGFALVKEYARQYLENLPRAYTINDVFYIATQQQLSEQAALAKSDSP
                     VVCDTDLTVIYVWLLYKYGICSKEMLDEISKSIPDTVYFLCYPDIEWEEDPLRENPDN
                     LLEIFEIYENLLKELGARFFVIQSDFDTRFQTCADVILEITHKM"
     gene            2336..>2912
                     /locus_tag="PLE23_11810"
     CDS             2336..>2912
                     /locus_tag="PLE23_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX83994.1"
                     /translation="MKFSIISLLLLAGNMVFGQAIISQRLEKIINESPESYQNVSLLL
                     SDRFDIVSLDNQLNAQRADQKTRVYQVITNLQEKAAHTQVDIIQFLKNSESVRPESIK
                     PLWITNIIFCTVKSSYIRTIATHPGISVIDLDAELMMVESKSEVAPPMPFAPNGAEPG
                     LKAIKADKLWALGYTGYGRKAYVADTGIDPMH"
BASE COUNT          805 a          558 c          625 g          924 t
ORIGIN      
        1 cgctgttgcg ccatttcgga gccactttta cggaaatttc caagaagact ttgcattgta
       61 taaaattttc gatatcgatg cgcgaggtag tggccagttt tttgattccg ccaccgtttt
      121 tgcccagtat gatggccttt tgtgattctc tttctactag cagctgcgct ctgatgcgtg
      181 ccagtggttg gccgtctttg gtggtagttt ctttgtaatc gtctatgact acctcagtcg
      241 tataaggaat ctcatcacgg tattgaagga aaactttctc tcgtattatc tcggtcacaa
      301 aaaatcgctc gagtcggtct gtcaactgat cggccggaaa atacatgggg ccatcgggca
      361 ggaacacttc aatgtgttca agcagggcat ttatgttgaa attgtttaat gcagaaatga
      421 gtacgacttc tttaacgggt atttgcgttt tccatttctc tatgagcagt tcgagttctt
      481 ccttgttgct gaggtcgatt ttgttaatga caagaatcac cggctccgaa acgctgttca
      541 tcaggtcgat aagtgggtct tcttcgccat agcggtcatc gggcgaagtc acaaacagga
      601 atacatcggc atcttcaaag ctggagtgca caaatttgtt catggctttg tgcattttgt
      661 atgccggttt ttcgatgaag ccgggcgtgt cactgaatac catctggtag ttgtctgtag
      721 taagtatgcc cagtatgcgg tgtcgggtgg tctgtggttt gggcgaaatg atggacatgc
      781 gttcgccgat gagtgcgttt atcagggttg atttgccaac gtttggcttg ccgatgatgt
      841 tgacaaaacc cgatttgaat ccttcttttg tttcggtgag ttccaaggtt taagtttaaa
      901 tttgttgttt aatattgcag tcggtttctg tgcgtattta ggtattgttc tattaattgt
      961 agtatttgct ttggcttgta gcgtctccag ttaacatcac tcaggttggc accaaggtaa
     1021 gcataatact gtatgtgtgt attgaccatc tcttcttcta cgtgtggcaa tgtgttgacg
     1081 aatgccatcc aacccatttc ttcagaaata tcttcatata ttgcttcata ataacaatcg
     1141 tctgacccgt ggaagttaag cacattttca caaataaaaa taaacctgtt gatgccgtgc
     1201 ttgaataata catctattac ttcttctttc agaaacttta tatcgttgta tagacaatca
     1261 ttccattcgc ctataaattc gagtatagcg aagccttcgt cgtagtcggc gtagagtatc
     1321 ttggtataaa gcgtgagcga accgaattcg tcccactgcg ggtgaatgta gtagttatat
     1381 attttattgt gataatgaaa ttcgtcatat tctctgccat aaaaaggcga atgctcgtct
     1441 tcttcagccg cataatcatc tctccaggca aaatatggtt ctatatcgtg catccgatgt
     1501 tcattaattt aattgcgata gcttattatt ttttaaaaaa tctctaccac attacaaaaa
     1561 taaaccgttt atttgagctg atggagaata tcagacgatt tgtttttaca ggacctgaaa
     1621 gtacgggcaa aactagcctg agtatcggca ttgcggatta tttcggcttt gcattggtaa
     1681 aggaatatgc taggcaatat ctcgaaaatc tgccacgagc ttataccatc aacgacgtat
     1741 tctacattgc gacacagcaa caactgtcgg aacaggcggc tcttgcaaaa agtgattcgc
     1801 ctgttgtatg tgataccgac cttactgtta tatatgtttg gttgttatat aaatatggca
     1861 tttgctcaaa agaaatgctt gatgaaataa gcaaaagcat acccgatacg gtctatttcc
     1921 tgtgttatcc tgatatagaa tgggaagaag acccgttgcg cgaaaatccc gacaatctgc
     1981 tcgaaatatt tgaaatatac gaaaatctgc tgaaagaatt gggagcaaga ttctttgtta
     2041 ttcaatcgga ttttgataca aggtttcaga cttgtgctga tgtaattcta gaaattactc
     2101 ataagatgta atgaaatttt gtattggcta ttgatgcttg aatataggcg caggtattat
     2161 cattaaaaat gttaatattg ggtacaagct caccgtattg ccagtttatg cacgaaaaat
     2221 tgggtgtatg aatctaattt gacgtaaatt gtgacgaatt cgcagatggt gatttgttta
     2281 ggataaatga ataccggttg atgaatcatt ttatgatcaa aaaacgcttt taaagatgaa
     2341 attttcaata atcagtttgt tgttgttagc cggcaatatg gtattcggtc aggctattat
     2401 ctctcagcgc cttgaaaaaa ttataaacga gtcaccggag tcatatcaaa atgtaagcct
     2461 tttattgtcc gacaggtttg acatcgtttc gcttgacaat cagctgaatg ctcaaagggc
     2521 agatcagaaa acccgggttt atcaggttat caccaatttg caggagaaag ccgcccacac
     2581 ccaagtggat atcatccaat ttcttaaaaa ttcagaatct gttagaccgg aatccatcaa
     2641 accactatgg attaccaaca ttattttttg tacagtaaaa tcttcgtata tcagaaccat
     2701 tgccactcac ccgggcataa gtgtgattga ccttgatgcc gaactcatga tggtagaatc
     2761 aaaatcagaa gttgcaccac cgatgccttt cgctccaaat ggcgctgaac ccggccttaa
     2821 agcaatcaag gcagacaaac tctgggcact cggttataca ggatatgggc gcaaggcata
     2881 cgtagcagat acaggtatag atccgatgca tc
//
LOCUS       DAOQQY010000319         2907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_17914_length_2907_cov_12.502454, whole genome shotgun
            sequence.
ACCESSION   DAOQQY010000319 DAOQQY010000000
VERSION     DAOQQY010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17914_length_2907_cov_12.502454"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2815)
                     /locus_tag="PLE23_11815"
     CDS             complement(<1..2815)
                     /locus_tag="PLE23_11815"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMX83995.1"
                     /translation="MLFLFNFAKTPFVYSQCGTLSVECSIEYPNALCVGTTVGIDEFS
                     DLINPSTSNQVYFVTGSFALNKSVSFTACTFVLDDDAQLIIGPAQNIQSVGYILFDVC
                     QIKACTKLWRNVLIKASWNMIFSSSKMSGSYEGIKLINGGNYLGRNNIFEDNYCSFTI
                     DQNTGVSKSTVAIYTSEFRSSGANLLPGVQSTSTRPHRAIYIRYDVPGFFVNNSYFHD
                     LQNGILLNNANMLGGISPNNVFKDIQPVDYPFDYAYLGNAISHIGSTKNVLLKMYGKY
                     NASNGQSGSYSVGPVNTPFTPNTIPLTFNQNFINCHRAIRIQDAKGDIQNNDMISCNY
                     GVVITLTGSGKQSEIKNNKIQDVKYVGIDFIGNKSGFASNVQSNKILFWPSGYTRIGI
                     RHLGVGPVPAQPVKIQYNTITIGTGNTNIYTIPVYGIDISGVAGTIVEQNNIKMDHIN
                     FNYIGIYLHRRSDNCIIENNWIEANDQSLPNIYVAQRVGILADESEQNEIICNRFTKL
                     GRCLNFLWECYETKVKSNIFVSGVQGLVYGDGFVEAVTGEQENMGNRWKNAFSKEQAI
                     YYNYASGLWFGQSNYIVHSNDFPVDIEYWPNSIEPNLPPPNEWFSEKTSLPAPPGGCM
                     TGLVPPGDGPLPELINDLITDNTSQWPNDAYFRRNMRWHTYNYLEGHPGLLIANTTAQ
                     DFLADEALTATRKLWNIRKMMAQASFIPESTQNVIDSLDNEKVGKAEQIINLLESRDT
                     LSLTQDFLDSLEILIDQYEMVISAGNVISEQIDSVQQNSYSQIANLLNALSVIDIMDV
                     NQKYTMGIELKRYINAEYRPDTTERNMLVYLASLCHRMEGPGVLEARNLLTVFGIEGV
                     MQDSTNCYTPIEPRERRSNQQASSSSGFTIYPNPGIASVSITLQNETDMENTVELRNS
                     VGELIMLQRTSAPKISLDVSGVPA"
BASE COUNT          824 a          653 c          542 g          888 t
ORIGIN      
        1 ccgctggcac acctgataca tctaagctaa tcttaggtgc agatgtgcgt tgtagcatga
       61 ttagctctcc gacagaattt cgcaactcaa ctgtattttc catatctgtc tcattttgca
      121 gcgtaatgct taccgatgca ataccagggt ttggatagat cgtaaacccg ctactgctcg
      181 atgcttgctg attagaccta cgttcacgcg gttctattgg tgtatagcag tttgtactat
      241 cctgcattac cccttctatg ccaaacactg tcaacaggtt ccttgcttca agtacccccg
      301 gtccttccat acggtgacac agcgaggcaa gatataccag catattccgc tcggtggtgt
      361 ccggcctgta ttctgcattt atatatcgtt tcagctctat acccatggta tatttctgat
      421 taacatccat gatatcaata acagacaggg cattaagcag attagcaatc tggctgtatg
      481 aattttgttg tactgaatcg atctgttcag atataacatt tccggcgctt ataaccattt
      541 catactgatc tatcaaaatc tccaaactat ctaaaaagtc ctgcgttaaa cttaaagtat
      601 cccgcgattc aagcaggttt ataatttgtt cagccttccc gaccttctca ttatctaatg
      661 aatctattac attctgtgtc gattcaggga taaaagatgc ctgggccatc atttttctaa
      721 tattccacag ttttcgagta gccgtcaggg cttcatctgc caaaaagtcc tgtgctgtcg
      781 tattggcaat caacaagccc ggatgtccct caagatagtt atacgtatgc catcgcatat
      841 tccgcctgaa ataagcatcg ttcggccatt gtgatgtatt atcggtaatc aggtcattga
      901 ttaactccgg aagtggacca tcaccaggtg gaaccagtcc ggtcatacat ccacctggag
      961 gggccggcaa ggatgttttt tcactaaacc attcatttgg tggaggtaaa ttcggctcaa
     1021 tgctgttcgg ccagtattct atatctacag gaaagtcgtt gctatgcaca atgtagtttg
     1081 attgaccaaa ccatagtcca ctcgcataat tataataaat ggcctgttct ttggaaaaag
     1141 cattcttcca gcggttcccc atattttcct gttcaccggt gactgcttct acaaatccat
     1201 ccccatacac caagccctgc actcctgata caaaaatatt cgacttgacc tttgtctcgt
     1261 agcactccca cagaaaattc agacaacgcc ccagctttgt aaacctgttg cagattatct
     1321 cattttgctc cgactcatct gctaatatac ccactcgctg ggctacatat atattcggca
     1381 gagattggtc attcgcttct atccagttat tctctatgat acagttgtca gagcgccgat
     1441 gtagatagat accaatataa ttaaaattaa tgtggtccat cttaatattg ttttgctcca
     1501 caatggtgcc tgctacacca gaaatatcta taccataaac aggtatggtg tatatattgg
     1561 tattgccggt accgatggtt attgtattat actgtatctt cacaggttgt gcaggcacag
     1621 gccccactcc caaatgcctg ataccgatac gcgtatagcc acttggccaa aacaatatct
     1681 tgtttgattg aacattagat gcgaatccac ttttattgcc tataaagtca ataccaacat
     1741 atttgacgtc ctgtatcttg ttattcttta tttccgattg cttgccgctt cctgtcaatg
     1801 ttatgactac tccgtaatta caagatatca tatcgttgtt ctgtatatca cctttcgcat
     1861 cctgaattct gatagcacga tggcagttta taaaattctg gttaaaagta agtgggatgg
     1921 tattcggtgt aaagggtgta ttcaccggac caaccgagta gctgccggac tggccattgg
     1981 atgcattata cttcccatac atctttaata atacattctt ggtacttcca atatgactta
     2041 ttgcatttcc caaataagca tagtcgaacg gataatcaac tggttgaata tctttgaaca
     2101 cattattcgg ggatatacct ccaagcatat tcgcattgtt gagcagaatg ccattctgca
     2161 aatcatgaaa ataactattg ttgacaaaaa atcccggcac gtcatatctg atataaatag
     2221 cacggtgagg ccgagtactc gtactctgca ctcccggaag cagattagca ccggatgacc
     2281 tgaactcgga tgtgtaaatc gcaacagtag atttgctcac acctgtgttc tgatcaatcg
     2341 taaacgagca ataattgtct tcaaaaatat tattacggcc taaatagttg cctccgttaa
     2401 ttaatttgat accttcataa gaaccagaca tcttggaact actgaatatc atgttccaac
     2461 tagcttttat caatacattc cgccaaagct ttgtacaggc tttaatttga catacatcaa
     2521 acaaaatata ccccactgat tggatatttt gtgctggacc aataattagc tgtgcgtcat
     2581 catcgagtac aaaggtgcag gctgtaaaac ttacactctt gtttaaagca aaacttcccg
     2641 taacaaaata gacttgatta ctggttgaag ggtttattaa atctgaaaac tcgtcaatac
     2701 ctacggttgt accaacacac aaagcattgg gatattcaat ggagcattca acagacagcg
     2761 taccacattg tgagtaaaca aaaggagttt tggcaaaatt aaacaaaaat aacattaaca
     2821 gtatttttcc atgccatttg aaaaaatcat actgtcgagg tgtcgaggtg tcgaggtgtc
     2881 gaggtgtcga ggtgtcgagg tgtcgag
//
LOCUS       DAOQQY010000320         2901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_17983_length_2901_cov_6.102249, whole genome shotgun sequence.
ACCESSION   DAOQQY010000320 DAOQQY010000000
VERSION     DAOQQY010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17983_length_2901_cov_6.102249"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1708)
                     /gene="aspS"
                     /locus_tag="PLE23_11820"
     CDS             complement(<1..1708)
                     /gene="aspS"
                     /locus_tag="PLE23_11820"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMX83996.1"
                     /translation="MYRTHNCGELRISDVGKEIILSGWVATSRDLGGLTFIDLRDRYG
                     ITQLTFNMDENAGLCQEARKLGREYVIKVSGTVRERSSKNANRPTGDIEIHVSRLELL
                     NASKVPPFTIEEETDGGDDLRMRYRYLDLRRPNVQQNLFFRSKLAIECRKFLAQQDFV
                     EVETPVLIKSTPEGARDFVVPSRMNKGQFYALPQSPQTFKQLLMVSGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVTQEDILNTFEGLTRHIFKSTLDVDLGEFERMKYDD
                     AMRLYGSDKPDLRFGMPFCELNDLVKDKGFQIFDAAELIIGICATGAAGYSRKQIDEL
                     TEWVKRPQIGAKGLVYIRCEEDGSYKSSVDKFYSQEDLAEVAGRCSAKAGDMILVLSG
                     DIDKVRKQLGELRLEMGSRLGLRNAKTFKPLWVVDFPLLEWDEDTSRFYAMHHPFTSP
                     KKEDITKMESGEHEALKALRADAYDLVINGVEIGGGSIRIHDRALQALNFKLLGFTPE
                     KAEEQFGFLMGAFEYGAPPHGGIAFGFDRLCAVMNGVNNIRDFIAFPKNNQGKDMMID
                     APS"
     gene            1833..>2901
                     /locus_tag="PLE23_11825"
     CDS             1833..>2901
                     /locus_tag="PLE23_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMX83997.1"
                     /translation="MRKMLLFFTLFFSLQSYTLVCQEGSEDQDAFFIKNLYTTALGQD
                     LSYDWLAGLTSQCAGRLAGSAQSEKAIRYTKAILDTLGLSKTWLQPCIVPVWERGDKE
                     QVKIMTSSRVTNRELNALALGFSGASPVQGITAEVVELHSIEETEKLGRKGLEGKIAF
                     FNRPFDRTMLHTFRGYGSAVDQRVYGPAKAAEYGAVAALVRSMTPNIDDVPHTGVTVF
                     KDSSKTIPAIAIATKDADLLSDLLQSGPVRVSVKTNCRTLPDKGSYNVIGEIKGSKYP
                     DEVIVVGGHLDSWDICQGAHDDGAGCVQSMEVLQLLLKTGYQPQRTIRCVLFMNEESG
                     SGGAKAYAEEAKKSNEKQYAAI"
BASE COUNT          749 a          701 c          624 g          827 t
ORIGIN      
        1 cagatggtgc atctatcatc atgtctttgc cctgattgtt cttaggaaat gcaatgaaat
       61 cgcgtatgtt gttgacgccg ttcatgacag cacaaagcct gtcgaatcca aaggcaatac
      121 caccatgcgg cggcgctcca tactcgaatg cacccatcaa gaaaccaaat tgttcttcag
      181 ccttctcagg tgtaaagcca aggagtttaa agttaagtgc ctgcaaggcc cgatcatgta
      241 ttcttattga gcctcctcct atctctacac catttatgac aaggtcataa gcatctgctc
      301 tcaaggcttt cagtgcttcg tgttcgccgc tttccatttt ggttatgtct tctttcttcg
      361 gtgaggtgaa cggatggtgc atggcataaa atcggctggt atcttcgtcc cattcgagca
      421 atggaaaatc gacaacccat aatggtttga aagtctttgc attccgcaac ccaagtctcg
      481 aacccatctc cagacgaagt tctccaagct gttttcttac cttgtcaatg tcaccggaga
      541 gtaccagtat catatcaccg gctttggccg aacatctgcc tgctacttct gccagatctt
      601 cctgcgaata aaacttatca acagatgatt tgtagctgcc atcttcttca cacctaatat
      661 ataccaatcc ttttgctcct atctgtggcc tcttgaccca ttctgtcagt tcgtctatct
      721 gtttgcggct gtagccggct gcacctgttg cacaaatacc aataatcaac tccgccgcat
      781 cgaatatctg gaaacccttg tcttttacaa gatcattcaa ttcacaaaat ggcattccga
      841 acctcagatc cggcttgtca cttccgtaca gacgcatagc atcatcgtat ttcattcttt
      901 cgaattcgcc caggtcaaca tcaagggttg atttaaaaat atgtctggta aggccttcga
      961 aagtattaag tatgtcttcc tgtgtaacaa aagacatttc gcagtctatc tgagtaaact
     1021 ccggctgacg gtcggctcgc aggtcttcat ctctgaaaca tttcactatc tgaaaatacc
     1081 ggtcaaatcc ggataccata agtagttgct tgaatgtctg tggcgattgt ggcaatgcat
     1141 agaactgtcc tttgttcatc ctgcttggca ccacaaagtc gcgtgcgcct tcaggtgtac
     1201 ttttgatgag gacaggtgtt tctacttcta caaaatcctg ctgcgcaaga aactttcggc
     1261 attctatggc aagttttgat ctgaaaaaca ggttttgctg cacatttggc cttctaaggt
     1321 cgaggtagcg gtatcgcatt cgcaggtcgt caccaccgtc tgtttcttct tcgatggtaa
     1381 agggaggtac ctttgacgca ttgagcagtt caagacggct tacgtgtatt tcgatgtcac
     1441 ctgttggcct gttggcattt ttactgctgc gttcgcgtac cgttccactc actttgatga
     1501 catattcgcg gccgagtttt cttgcctctt gacacagtcc tgcattttca tccatattga
     1561 atgtcagctg tgttatccca taacggtctc tcaggtctat gaaagtaagc ccaccaaggt
     1621 cgcggcttgt agcaacccag ccactcagaa taatttcttt tcccacatct gaaattctca
     1681 gttctccaca attatgcgta cggtacatat ctttttattt gttattttta atgaaatatg
     1741 gttgcaaaga taaacattga tttacatgaa aatctattct tattctttgc ttggttttta
     1801 aaatgcttaa cttggtgtca aattttaata aaatgagaaa aatgctcctc ttttttactt
     1861 tgtttttcag cctgcaatct tatacgttag tttgtcagga agggtctgaa gatcaggatg
     1921 ccttttttat taaaaattta tataccactg ctctcgggca agacctatca tacgactggc
     1981 ttgcaggtct tacctcgcag tgtgccggcc gcctggcagg ctcagcccag tcagagaaag
     2041 ccatacgtta caccaaggcc atacttgata cgctcggcct cagcaagacc tggttacagc
     2101 catgcattgt tccggtatgg gagcgtggcg ataaagaaca ggtcaaaatc atgacttcaa
     2161 gccgggtaac aaacagagaa ctgaatgcac ttgcactcgg tttttcgggt gcttctccgg
     2221 tacaaggtat cacggcagag gtggtagaac tacacagcat cgaagagaca gaaaaacttg
     2281 gccgcaaagg actggaaggt aaaattgcat tcttcaaccg accatttgac aggacgatgc
     2341 ttcacacctt ccgcgggtat ggctctgcag tggatcagcg tgtgtacggc cctgcgaagg
     2401 cagccgaata cggagctgta gctgcactcg tccggtctat gacaccgaat atcgatgatg
     2461 tacctcatac cggcgtaaca gtattcaaag acagtagcaa aaccatcccg gccattgcca
     2521 tcgccaccaa agatgccgat ttgctatccg atttgctaca atcaggaccc gttagagtgt
     2581 ctgtaaagac caattgccga acattgcccg acaagggctc ttataatgta atcggtgaga
     2641 taaaaggctc aaaatacccg gatgaagtga tagtagtagg cggccacctt gactcttggg
     2701 acatctgcca gggtgcgcac gatgacggtg ccggatgtgt ccagtcgatg gaggtactgc
     2761 agttattact caaaactggc tatcagcctc agcgtaccat acgttgtgta ttatttatga
     2821 atgaagaaag cggttcaggt ggcgctaaag cctacgcaga ggaagcaaag aaaagtaacg
     2881 aaaaacaata cgccgccata g
//
LOCUS       DAOQQY010000321         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18060_length_2893_cov_6.633897, whole genome shotgun sequence.
ACCESSION   DAOQQY010000321 DAOQQY010000000
VERSION     DAOQQY010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18060_length_2893_cov_6.633897"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PLE23_11830"
     CDS             <1..1030
                     /locus_tag="PLE23_11830"
                     /inference="COORDINATES: protein motif:HMM:NF020260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HMX83998.1"
                     /translation="NHAILSGYDKLLPEDTYPFYTIFLDIDPSNIDINVHPTKQEIKF
                     EDEKLVYNYLFVAVKHALGKHNITPLLDFEQEAAFNTYQSGMSNKKPGHDSYSNGYNS
                     QKNTDNNLKNWELLYSQQDNQTDHEPSNGSFTIGTEWHHTDAQEKLHINQEVSRKAPY
                     QIHNTYIINQIKSGFIVIDQQSASEQILYEKFLHTLNENQGLAIQKLLFPHTIHLSPA
                     DFAIASRILHSLNQLGFDIQEFGKNSLVVHGMPAEIENSENPEKLLEEFIGQIKEGFE
                     LKTNLSENLAKVLARQMSVKRGKPLNEETMKELIDSLFACKIPYQSPFGRKCFVTFEL
                     NDFEKLFA"
     gene            1069..1653
                     /locus_tag="PLE23_11835"
     CDS             1069..1653
                     /locus_tag="PLE23_11835"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015692542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMX83999.1"
                     /translation="MIRLTEVVKQILIINVLVFLAQMTLGNGSFVLMYPADEAFRPYQ
                     ILTHMFMHGGFTHILFNMFSLAMFGPPVEARIGPKKFLFYYLFCGVGAAILQLFVIYL
                     EFGFTPVQYGMLGASGAIFGILAAFGWYFPNVQMQMIFPPVAMRAKYMVILFGVLELV
                     MGVGGYAQGIAHFAHIGGAVFGLLLILYWRKFDR"
     gene            1726..2613
                     /locus_tag="PLE23_11840"
     CDS             1726..2613
                     /locus_tag="PLE23_11840"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMX84000.1"
                     /translation="MLSKLVIVNVAAFLLIGLVWFIGMITHAQAIQSAYQEIVNQLGV
                     TESFMQNLRKPWTYVSYMFMHSGIFHLGWNMILFVWFGRIIGDFLGDRRILPIYLMGG
                     LSGVIFLMLVAAITNLAVLNNVLMIGASASVMAFAMAAATLSPDYSYRLLFFGDIRLK
                     YIVLALMIIDVVYMSVGNNVAGHLSHFGGAIFGWAYIVLLRRGTDLTEPLQKFFKIIT
                     DKATNIEARGHSRIKKRVLQGEEYQAVNRSQQKSHDEHQLDFEERLDNILEKIKKKGY
                     DNLEHEEKEFLNKASNKQD"
     gene            2637..>2893
                     /locus_tag="PLE23_11845"
     CDS             2637..>2893
                     /locus_tag="PLE23_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84001.1"
                     /translation="MNIALAVITLLSYLAPNINPEKFWPAAMFGLIFPVLVILNLLFI
                     LFWVFNKNMLVLISLATVVIGFNSVKGVINFGSKNQRTTGK"
BASE COUNT          902 a          534 c          575 g          882 t
ORIGIN      
        1 caaccatgct atactttcag gttatgacaa attattgcca gaagatactt atccttttta
       61 tactatcttt cttgacattg acccctccaa tatagatatc aatgtacacc cgaccaagca
      121 ggaaataaaa tttgaagatg aaaaactcgt gtataattac cttttcgtag ctgtcaaaca
      181 cgctttaggc aagcataaca ttacaccact ccttgatttt gagcaagaag ccgccttcaa
      241 tacttatcaa tcaggtatgt caaacaaaaa accgggtcat gattcatatt ctaatggata
      301 taattcacaa aaaaatactg acaacaacct caaaaactgg gagcttctat attctcaaca
      361 agataatcag acagaccatg agccatcaaa tggctctttc acgataggca ccgaatggca
      421 tcatacggat gctcaggaaa aactgcacat caaccaggaa gtaagcagaa aagccccata
      481 ccagatacat aatacttaca tcattaacca gataaagtca ggctttattg tcattgacca
      541 gcagtcagct tcagaacaga tcttgtatga aaaattttta catacattaa atgaaaatca
      601 ggggttggca atacaaaaac tgttatttcc acacactatt cacctgtcgc cggctgattt
      661 tgctatagca tccaggattc ttcacagtct taaccaactt ggattcgata ttcaggagtt
      721 tggtaagaac agcctggtag tacacggtat gccggcagaa attgagaatt cggaaaatcc
      781 cgagaaatta ctggaagaat ttatcggtca gataaaagaa ggatttgagc ttaaaaccaa
      841 cctgagcgaa aatttggcaa aagtgctcgc cagacaaatg tcggtaaaaa gaggaaaacc
      901 actcaatgaa gaaaccatga aagagttgat tgacagcttg tttgcatgta aaatacctta
      961 ccagagtcca tttggcagaa aatgttttgt tacctttgag ctgaatgatt tcgaaaaact
     1021 atttgcctga attatagaac actattaaac aattaagcta agtatcacat gatcagatta
     1081 acagaagtag tcaaacaaat actcattatt aatgtacttg tgtttcttgc acagatgaca
     1141 cttggcaacg gctcatttgt actgatgtat ccggcagatg aggcattcag accataccag
     1201 atacttacac acatgtttat gcatggtggt tttacacaca tattgttcaa tatgttttcg
     1261 cttgcaatgt tcggtccgcc cgtagaggcc cggatcggac caaagaagtt tttgttttat
     1321 tatttgtttt gtggcgtagg tgcagccatt ctgcaattgt ttgttattta tctcgagttc
     1381 ggttttacgc ctgtacaata cggaatgtta ggagcatcgg gagctatttt tggcatactt
     1441 gctgcctttg gctggtattt ccctaatgta cagatgcaga tgatttttcc acctgtggca
     1501 atgcgagcca aatacatggt aatattgttt ggtgtgctcg agctggtgat gggtgttggt
     1561 ggctatgcac aaggtattgc acactttgca catattggtg gcgcggtttt tggtttgctt
     1621 ttgatattat attggcgtaa atttgaccgg taaataaatt ttactatttt aataaatatg
     1681 tactcatcca tcatatctga tatcaggcgt gcttttcata gcaatatgtt aagtaaactc
     1741 gtaatagtca atgtagcagc atttctgttg atagggcttg tttggtttat cggaatgata
     1801 actcatgcac aagccataca aagcgcatat caggaaatag taaaccaact tggtgtaaca
     1861 gaaagtttta tgcagaatct gcgcaaacca tggacatacg tttcctatat gtttatgcac
     1921 agtggtatat ttcacttagg ttggaacatg atattattcg tctggtttgg tcggataatt
     1981 ggtgattttc ttggtgacag gcgaatattg cccatttatc tcatgggtgg actttcggga
     2041 gtcatatttt tgatgttggt agcagccatt accaacttag ctgtactaaa taatgtttta
     2101 atgataggag cttctgcttc tgttatggca tttgctatgg cggccgctac tctgtcaccc
     2161 gactatagtt acaggcttct tttttttggt gacatcaggt taaaatatat cgttctcgcc
     2221 ttaatgatta ttgatgttgt ttatatgtct gttggcaata atgttgcggg acacttgtca
     2281 cattttggcg gtgccatatt cggttgggca tatattgtgt tactgcgaag aggtaccgac
     2341 cttactgaac cgttacaaaa attcttcaaa ataataactg ataaagctac caatatagaa
     2401 gcacgcggac attcgagaat aaagaaacga gttttgcaag gcgaggaata ccaggctgtg
     2461 aatagaagcc agcaaaaatc gcacgatgaa catcaacttg attttgaaga aagactagac
     2521 aacattttag aaaaaatcaa aaagaaagga tacgataacc tcgaacatga ggagaaagaa
     2581 tttttaaata aggcaagcaa caaacaagat taactttgat caaaattgtc aaatgggtga
     2641 acatcgcttt ggcagttatc acactgctta gctatctggc ccccaacatc aacccagaaa
     2701 aattttggcc ggcagcaatg tttggtttga tctttccggt tttagttatt ctgaatttgc
     2761 tcttcatcct cttttgggta tttaacaaaa atatgctggt actcatttca ttagccaccg
     2821 tggtcattgg ctttaatagt gtaaaaggtg taataaactt tggcagcaaa aatcagcgta
     2881 ctacaggtaa gag
//
LOCUS       DAOQQY010000322         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18128_length_2886_cov_5.862239, whole genome shotgun sequence.
ACCESSION   DAOQQY010000322 DAOQQY010000000
VERSION     DAOQQY010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18128_length_2886_cov_5.862239"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLE23_11850"
     CDS             complement(<1..587)
                     /locus_tag="PLE23_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX84002.1"
                     /translation="MQNIILKADKISKSFHDPVSVKILHEVSFEIRRSEFVSIIGKSG
                     CGKSTLLYILSTMDTDYTGNLEIDGEKMATKREKELAHIRNTKIGFVFQFHYLLNEFT
                     VLKNVMLPALKLNTLTSEEIAENALQKLNILGIKDLASKFPNQLSGGQKQRVAIARAM
                     INEPSIIMGDEPTGNLDKNNSRIVFETFKQLATEFG"
     gene            complement(589..1848)
                     /locus_tag="PLE23_11855"
     CDS             complement(589..1848)
                     /locus_tag="PLE23_11855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX84003.1"
                     /translation="MRFKLLTEIAMTMLKARLKQTLVAAVGVTFGITMFVTLLSFMNG
                     LNNLLDGLIVNRTPHIRLFNEIEPKQNQPIKLIEQYKDDYHFISSVKSSNSRLEIYNS
                     EKIIEALGHDKRVKGVSRKASTQVFFNDGAIDISGILNGIDVDKEISYYKFEDYVTSG
                     KPSDLKNVPNSIILGKPLAEKLLANIGDVIQLTTPQGEKFRMKVMGYYQSGYNDFDKT
                     QSFVSLSNLQKIIGKSSNYVTDIQIKLHDIKDATYMAGEYHEKFEVKAEDIQTANAQF
                     ETGTKIRTLISYAVGITLLIVAGFGIYNILNMMIYEKMDTIAILKATGFSGADVKTIF
                     MLISLSIGFFGCIAGLFLGNMLCRLISSIPFNTAALPTVKTYPVSFEPAYYIIAFIFA
                     IVTTFFAGWFPSRKASKVDPVIIIRGK"
     gene            complement(1848..>2886)
                     /locus_tag="PLE23_11860"
     CDS             complement(1848..>2886)
                     /locus_tag="PLE23_11860"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMX84004.1"
                     /translation="IACKNKQESTRPEYRNITQSVYASGIIKSREQYQVFTTISGIIK
                     KVFITEGEYVQKGQMLFQIKNEAAEISKENTGIVADFNKIKNNQDKLAELQSSIDLAR
                     QMRENDSLLFVRQKNLWASGIGSKAELERKELNAKNSKTNHTNALLKYSELKKQLRFA
                     EKQSIKQYEISASQAGDLYIRSLLGGNVLSVFKKTGELATTQQPLAIISDTAHYYLEL
                     QLDEYDIASTEVGMKVFVTMDSYRGDVFEAKLTKIYPVMNDRTKTFTAEAEFVKAPPK
                     IYPNLTVEANILIKEKKNALLIPRSYLLDEKYVLLENGKKAEIKTGIKNYEKVEIISG
                     VNQNDVILKPK"
BASE COUNT          730 a          564 c          569 g         1023 t
ORIGIN      
        1 ccgaattctg tggcgagctg cttgaatgtt tcgaatacta tgcggctgtt gttcttgtcg
       61 aggttgcccg taggttcgtc acccataatg atgctcggtt cattgatcat ggcacgagct
      121 atggctacac gctgtttctg gccgcctgat agctggtttg ggaattttga agcgaggtct
      181 tttataccga gaatgttgag tttttgaaga gcattttccg cgatttcttc agatgtgagt
      241 gtattcagtt ttaaagccgg caacattaca ttttttaata cagtgaattc gtttagcaaa
      301 taatgaaact gaaatacgaa gccgattttc gtattacgaa tgtgagcaag ttctttttct
      361 cgtttggtgg ccattttttc accatctatt tcaagattgc ctgtataatc cgtgtccata
      421 gtagagagaa tgtacagcaa ggtcgatttg ccgcaacccg acttgccgat gatagacaca
      481 aactccgacc ggcgtatttc aaagcttact tcatgtagaa tttttaccga taccggatca
      541 tgaaagcttt tgcttatttt atcagccttg agtataatgt tctgcatatt atttccctct
      601 gatgataatg accggatcaa ccttgcttgc ctttctggag ggaaaccatc ctgcaaaaaa
      661 tgtagtgacg atggcaaaaa tgaaagcaat gatataataa gcaggctcga agctcactgg
      721 ataagtctta acggtaggca aggcagcagt attgaaggga atgctgctga taagccggca
      781 gagcatattg ccaagaaaca gcccggcaat gcatccaaag aaacctatac taagtgaaat
      841 gagcataaat attgtcttga catctgcacc tgaaaatccg gtagccttca atattgcaat
      901 ggtatccatt ttttcataaa tcatcatatt gaggatgttg tatatgccaa atcctgctac
      961 aatgagtaag gtaataccta cggcataaga gatcagcgtg cgtattttgg tgccagtttc
     1021 gaactgtgca ttggctgtct ggatatcttc ggctttcact tcaaattttt catgatattc
     1081 acctgccata taagttgcgt ctttgatatc gtgaagtttg atctgtatat cggtcacata
     1141 attactactt tttcctatga ttttttgaag gtttgacagt gaaacaaagc tttgtgtttt
     1201 gtcgaaatca ttgtagcctg actggtaata tcccattact ttcattctaa atttttcacc
     1261 ttgcggagtg gtcaattgga tgacatcgcc gatgttggca agcagctttt ctgccaatgg
     1321 ttttccaaga atgatgctgt tgggtacgtt cttcagatca gacggtttgc cggaggtgac
     1381 ataatcttca aatttgtaat aagatatttc tttatctaca tcaataccgt tcagtatgcc
     1441 actgatatct atggctccgt cattaaagaa aacctgtgta gatgcctttc gtgatacacc
     1501 tttgaccctt ttatcatgac cgagtgcttc tattattttt tcagaattat aaatctcaag
     1561 cctgctgttg cttgatttga cggatgaaat gaaatgataa tcatccttgt attgttcgat
     1621 taattttatt ggttggtttt gtttgggttc gatttcattg aataacctaa tgtgtggtgt
     1681 tctgttgact ataagcccat cgagcaggtt gttgaggccg ttcataaaac tcaacaaggt
     1741 aacaaacatg gttatgccaa aagtgacacc tacagctgct accaaggttt gttttaacct
     1801 tgctttaagc atggtcattg ctatctcggt caataactta aatctcatca ctttggtttg
     1861 agtatgacat cgttttgatt tactccactg attatttcta ccttttcgta gttttttata
     1921 cctgttttta tttctgcttt tttaccattt tcaagcaata cgtacttctc atcaagcaaa
     1981 taactccttg gtatcaataa ggcatttttc ttttctttta tcaggatgtt tgcttctacg
     2041 gtcaagttag ggtaaatctt aggtggagct tttacgaatt ctgcctctgc ggtgaaggtc
     2101 ttggtacggt cattcatcac cggatagatc ttggtcaatt ttgcttcaaa aacatcccca
     2161 cggtaactat ccatcgttac gaatactttc attccgactt ctgtggatgc aatatcgtat
     2221 tcatcaagct gcagttcgag ataataatgt gctgtatcgc ttatgatagc aagtggctgc
     2281 tgggttgtcg cgagctcacc ggtttttttg aacacactca atacattacc gcctaacaga
     2341 ctcctgatgt acaagtcacc ggcttgcgag gcacttattt cgtattgttt gattgattgt
     2401 ttttctgcga aacgcaactg ttttttgagt tcggaatatt tcaacagcgc attggtatgg
     2461 tttgttttgg aatttttcgc atttagttcc tttcgttcaa gttcggcctt gcttcctatt
     2521 cccgatgccc acagattctt ttgtctcaca aacaaaagtg aatcattttc cctcatttgt
     2581 cttgccaaat ctatacttga ctggagttcg gccagcttat cctgattgtt ttttattttg
     2641 ttaaaatcag ccactatccc tgtattttct ttggatattt cagcggcttc gttttttatt
     2701 tgaaacaaca tttgcccttt ttgcacatat tctccttctg taatgaagac ttttttgatg
     2761 attcctgaaa tagtcgtaaa aacctgatat tgctcacggc ttttaattat tccggaggca
     2821 taaacggact gagtaatatt tcgatattcg ggtcttgtgc tttcttgttt atttttacag
     2881 gcaatg
//
LOCUS       DAOQQY010000323         2878 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18226_length_2878_cov_4.796670, whole genome shotgun sequence.
ACCESSION   DAOQQY010000323 DAOQQY010000000
VERSION     DAOQQY010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18226_length_2878_cov_4.796670"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..1333
                     /locus_tag="PLE23_11865"
     CDS             74..1333
                     /locus_tag="PLE23_11865"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX84005.1"
                     /translation="MNLKNSEFFSALQNVDFRYFLSVRFLATFAAQMTSTLLAWQVYK
                     ITRDPLALGLIGLFEVLPFISMTLFAGHYVVKYSRKLIIVISATVLLACSVALALFTS
                     SGMLTELKYGIWLAYSVNMVTGIARAFLFPAMTALRANIIRGPHMQNSIAWNVNIWYL
                     AAISGPALAGLLYDWVGVVTAYWISVAIFSVAIFSVFMIREKGLSKGQHSEDIWKSLK
                     EGINFVFERKIILSAITLDLFAVLFGGVVALLPVFAQEVLHTNATGLGILRAAPFVGS
                     LLSGIVLAARPIRKNVGVILLTCVALFGVANLLFSLSEYLWLSAVLIGLAGVFDNVSV
                     FIRHTIMQLYVPDNMRGRVSAVEGIFISSSNELGSFESGFAAKILGLIPSVVFGTSMT
                     FLSVLLANRLSPELKQLDWSNESQQIE"
     gene            1478..>2878
                     /locus_tag="PLE23_11870"
     CDS             1478..>2878
                     /locus_tag="PLE23_11870"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HMX84006.1"
                     /translation="MNAYSQTAPFDFSGMDKSVKPGDDFNMYVNGQWFKNTEIPSDQS
                     RWGSFMILREENNKRLRSIMEEAAANKTTTDINIRKIGDFFASAMDTVQIEKLGSTPV
                     RTYMQRIDIIKNIRDLVKECGYMHTLGLSPLYSFYAYQDDKNSAKVVAQFHQPRLGLP
                     EKGYYFDQDEHKKKIREAYTEYITKLFAGAGYTKERAAANASMILALETKLAGASKSA
                     IELRDPEANYHKMSSKDFGALISHISFQELQASLLIQQDTFIVGQPDYFKTLNDVILS
                     TPFNDLKEYLKFGYLSGLAPYLSKDFQKISFNFSKEFSGQKVQQERGKRMVDLINGKL
                     GEMIGQIYVQKYFPPEAKSRMLELVHNLQSAYETRINAAEWMSDVTKKKAIVKLKGML
                     LKIGYPDQWKDYAALQISRNNFVQNIINSNVFEYNEMVEKLKKPVDKTEWLMSPAMVN
                     AYYNPQTNEIAFPAGIL"
BASE COUNT          849 a          565 c          609 g          855 t
ORIGIN      
        1 gtagaaaaga acacgatata tgcttttttg caaaccgaaa tcggatattt gtattataaa
       61 gcaagccata caagtgaatt taaagaacag tgagtttttt tctgccttac agaatgttga
      121 tttcagatat ttcctgagtg tcaggtttct ggctactttt gctgcacaaa tgacatctac
      181 tttgcttgcc tggcaggtgt acaagataac acgcgatccg cttgcacttg ggcttattgg
      241 cttgtttgaa gttttgcctt tcatttcgat gaccttattt gccggtcatt atgtagttaa
      301 atattcaagg aaattaatta ttgtaatatc agccacagtg ctgcttgctt gctcagttgc
      361 acttgctctc ttcacttctt cgggtatgct cactgagctg aagtatggta tctggctggc
      421 atattcagta aatatggtca cgggtatagc ccgggctttt ctttttccgg ccatgacagc
      481 actacgtgcc aatatcattc gggggcctca catgcaaaac tctatagcct ggaatgtcaa
      541 catctggtat ctggctgcaa tatctggtcc tgcactcgcc ggcctgcttt atgactgggt
      601 gggtgtagtt actgcttact ggatttccgt tgctattttt tcagtagcca ttttttcggt
      661 ttttatgatc agagaaaaag gtttgtcaaa ggggcagcat tcagaagata tctggaaaag
      721 cctgaaggaa ggtatcaatt ttgtattcga acgcaaaata atactcagtg ccattacact
      781 tgacttgttt gcagtattgt ttggtggtgt tgttgctctg ttaccggttt ttgcacagga
      841 ggtgctccat accaatgcta ccggtctcgg aatactaagg gcagcgccat ttgtaggatc
      901 attgctatcc ggtattgtgc tggcagcccg acctatccga aagaacgtag gcgtaatctt
      961 gctcacatgt gtagcattgt ttggtgttgc caatctgctc ttcagccttt cagaatatct
     1021 ttggctctct gcagtgctga ttggtctggc aggtgtgttc gacaacgtaa gcgtatttat
     1081 taggcatacc attatgcagc tctatgtgcc ggataatatg cgtggccgtg tttctgccgt
     1141 agaaggaatt tttatttcat cttcaaacga acttgggagt tttgagtcag gttttgcagc
     1201 aaaaatatta ggccttatac cttccgttgt ctttggtaca agtatgactt tcctgtctgt
     1261 acttttagct aaccgtttat cacccgaact caagcaactc gattggtcaa atgaatcaca
     1321 acaaattgag taaagtattt caatcttaaa aatcactttg ttaatctaaa tattgtactc
     1381 aattgaataa aattgagcat ttttgcaccc gaaaaaaact gaaattctta tgatcaaaaa
     1441 attaactttt attgttgtca taatacaatt aacaattatg aacgcataca gccaaacagc
     1501 ccctttcgat ttttccggaa tggataaatc ggtaaaacca ggtgacgact tcaatatgta
     1561 cgtcaacgga caatggttca aaaatactga aatcccttct gaccagtcac gttgggggtc
     1621 gttcatgatt ttgcgtgaag agaacaacaa acgtcttcgc agcatcatgg aagaggcagc
     1681 tgccaacaaa acaacaactg acatcaatat acgaaaaatt ggcgatttct ttgcaagtgc
     1741 tatggatact gtgcagattg agaaactcgg tagtacaccc gtgagaacct acatgcagcg
     1801 gatagacatc atcaagaata ttcgagatct ggttaaggaa tgcggttata tgcacacgct
     1861 tggattgagc ccgctttatt cattctacgc ttatcaggat gacaaaaaca gtgcaaaggt
     1921 tgtagcacag tttcatcagc ctcgtcttgg tttgcctgag aaaggttact attttgacca
     1981 ggatgaacac aagaaaaaaa ttcgcgaagc atacactgaa tacatcacaa aactatttgc
     2041 gggtgcgggt tatacaaaag aacgagctgc tgccaatgcg tccatgatat tagcacttga
     2101 aaccaaactg gcaggtgcat caaaaagtgc tatcgaattg cgcgatccgg aggcgaatta
     2161 ccataaaatg agttctaagg atttcggagc acttatttcg catatcagtt ttcaagaatt
     2221 gcaagccagt ttgctcatcc agcaggatac attcattgta ggtcagcctg attacttcaa
     2281 aacgctgaat gatgtcattt tatcaacacc tttcaacgac ttgaaggaat atcttaagtt
     2341 tggttatctg tcaggtctgg ctccttatct aagcaaagat tttcagaaga tatcctttaa
     2401 cttcagcaaa gaatttagcg gacaaaaggt gcagcaggaa agaggtaaaa gaatggttga
     2461 tctgatcaat gggaaactgg gcgaaatgat aggtcagatt tacgtacaaa agtattttcc
     2521 gccggaagcg aaatcaagaa tgctcgaact tgtacacaac ctacagtcag cttacgaaac
     2581 acgtataaat gcagctgaat ggatgagtga tgtgaccaag aagaaagcca ttgtcaaact
     2641 gaaaggaatg ttgctcaaaa tcggataccc cgatcagtgg aaagattatg ctgcattaca
     2701 gatttcaaga aataattttg tacaaaatat catcaactct aatgtatttg aatacaatga
     2761 aatggtcgaa aaactcaaaa aaccggttga caaaacggaa tggttgatga gtccggccat
     2821 ggtaaatgct tattacaatc cacagaccaa cgaaatcgct ttcccggcag gtattctc
//
LOCUS       DAOQQY010000324         2874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18269_length_2874_cov_4.195105, whole genome shotgun sequence.
ACCESSION   DAOQQY010000324 DAOQQY010000000
VERSION     DAOQQY010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18269_length_2874_cov_4.195105"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2732)
                     /locus_tag="PLE23_11875"
     CDS             complement(<1..2732)
                     /locus_tag="PLE23_11875"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX84007.1"
                     /translation="MSGQKYSFSFVGATRNAYEDTDTSIIYNEAAPIDFKIRLAGEVP
                     SDLPGGLCGDYPIQSSNQIIMEEDEFSQYEWGDIFKCDFVANDNYTNIVFTATNDEEG
                     ERSLLFFDNVKLWCESQREANIGFAQSSWNVFSFAGNTTGPDDVTVSEYKWSFGDGKT
                     ASGQNVTHEYAASGEYFVTLCITDNRGCTSSVTQRILMYNEAECTCPNNQIDLGVTNE
                     STLVSQTPIGINSSYNNAGHCISIKGELVIDKSFELKNGEIIMHPGSSISIPGNSGFE
                     LKLTGSNNPSPSLNIHGCAGIMWKGIDANNNKITIRETIISDAENAILCTDGASVKSY
                     YSIFQNNWIGLRLSGNLFLVGYIDTDIKGTSFLGKGSMNIPFTENLSAYMPVKPYAGI
                     VATNLIYTNLKAVNGAFFAPFADLPNTYKDMENGILSQGSDVDVNHSDFINITKYPYN
                     IYTPSGNGIFTAPLHGYSSNISGLTILGKKPCNFMNCTNGIQSYRSNLFVEKATFVDC
                     QYGINATDPVNKYLYIKNCTMTKIANTGISVNSTYGVSGTTTSGTIAQNIISLDGSGT
                     SLYKRAIHLNLNAKYSFYVEENHINTDNFSLGHGIFEENCSNINYVKNFIDIESPLFG
                     GILSRNSNNNLYNCNSVRGISTSPSFKYWHNAYTFANNSTSVIECNGSDNTTYGLYFD
                     GPNDLTNRIITNEMGTHYVGLCYKDAGSQTGAQYYTGNTWSNYNGPSVYQAFHGGGDA
                     ASLYSTYIVPNLPQFNPNPVYPDKDWFIASGQQTKYCEPACVGSFVSPEFGRLDSLVT
                     VQAFDEDGNYPAMQYILEKMVMYKVLENPELQVSPVAQVFYNTRIHTNAGKFARIKYM
                     VEGAGMADTALWNAYADKYDEAQNLKLIIRQYEAFIADTSGNVTDSLIVLRKHY"
BASE COUNT          836 a          597 c          500 g          941 t
ORIGIN      
        1 atgtaatgct tccgcaatac aatcaggcta tcagtcacat ttccggatgt gtcagcaata
       61 aaggcctcgt attgtctgat gattaacttc aggttttgag cctcatcata tttatcagca
      121 taagcattcc acaatgcagt atctgccata ccagcacctt ccaccatata tttgatcctg
      181 gcaaattttc cggcattggt atggatgcga gtattataaa aaacttgagc caccggtgat
      241 acttgcagtt ccgggttttc aagcactttg tacatcacca ttttttctaa tatgtattgc
      301 atggctggat aattgccatc ttcatcgaaa gcttgaaccg tcacaaggct gtcaagtctt
      361 ccaaattccg ggctgacaaa actcccaaca caagccggct cgcagtattt cgtttgctgc
      421 ccggaagcta tgaaccaatc cttatcagga tatactggat tggggttaaa ctgtggcagg
      481 ttaggtacta tatatgttga atatagagaa gccgcgtcac cacctccatg aaaagcctga
      541 tagaccgacg ggccattata attggaccag gtgttgccgg tataatactg ggcccctgtt
      601 tgacttccag catccttata gcatagccct acataatgag tacccatctc gttggttatg
      661 attcggtttg taagatcgtt cggtccatca aaatacaggc cgtaggttgt attatccgat
      721 ccattacatt caataacgga agtactgtta ttggcaaacg tataggcatt atgccagtat
      781 ttaaaagacg gggaagtact tattcctctc actgaattac aattatatag gttattgttc
      841 gagttgcgtg acaagattcc accaaagagg ggtgattcta tatctataaa attcttgaca
      901 taattaatat tgctgcaatt ctcttcaaat ataccatgac ctaatgaaaa attatcggtg
      961 ttgatatgat tttcctcgac ataaaaagaa tacttggcat ttaggttaag gtggatggct
     1021 cttttatata atgatgtacc gcttccgtct aaactaatta tgttttgagc aatggttcct
     1081 gaggtggtag taccactcac cccataagta ctattaacac ttatcccggt gtttgcaatt
     1141 ttggtcattg tacaattttt aatatacaaa tatttattga ctgggtctgt tgcatttatg
     1201 ccgtattgac agtcaacgaa tgtagctttt tccacaaata gatttgaccg atacgattgg
     1261 attccattgg tacaattcat gaaattgcac ggtttttttc ctaatatggt cagaccactg
     1321 atattacttg aatatccatg tagaggggca gtaaatatgc catttccact tggtgtataa
     1381 atattataag gatattttgt aatatttatg aaatcagaat ggtttacatc aacatcactc
     1441 ccttgagaaa gtatcccgtt ttccatgtct ttataagtat tcggcaaatc cgcaaaaggt
     1501 gcaaaaaacg ccccatttac agcttttaga ttggtataaa ttaaattagt tgcaacaatg
     1561 cctgcgtaag gttttaccgg catgtatgct gataaatttt cagtgaaagg tatattcata
     1621 ctgcctttgc ctaaaaagct tgtgcccttg atatcagtgt caatataacc taccaagaat
     1681 agattcccag acaatcttaa tcctatccaa ttattctgaa aaatgctgta ataagatttt
     1741 accgacgctc catcagtaca aagaattgca ttttcagcat ccgatattat tgtttccctt
     1801 attgtaatct tattattatt agcatctata cccttccaca ttatgccggc acaaccatgt
     1861 atgttcaaac ttggcgaagg gttgttactt ccagtcaatt ttaactcgaa accgctatta
     1921 cctggtatgc ttatgcttga tcctggatgc ataattatct caccattttt caattcaaaa
     1981 ctcttgtcaa taaccagttc tccctttatc gatatacaat gtcccgcgtt attgtaagac
     2041 gaattgattc ctataggtgt ctgggaaact aaagttgatt catttgtcac gcctaagtct
     2101 atctgattat tcgggcaagt gcattccgct tcattataca ttaaaatacg ttgcgtcaca
     2161 ctacttgtgc atcctctgtt atctgtaata cataatgtaa caaaatattc cccacttgca
     2221 gcatattcat gcgtcacatt ttggccagaa gctgttttac catctccaaa actccatttg
     2281 tactcactta cagttacatc atccgggcct gttgtgtttc ctgcgaaact aaaaacattc
     2341 catgaacttt gtgcaaatcc tatattcgct tctctttgtg actcacacca taatttaacg
     2401 ttatcaaaaa ataataagct tcgttcgcct tcctcatcat tggttgcagt aaaaacaatg
     2461 ttcgtataat tatcattagc cacaaaatca catttaaaaa tatcccccca ttcgtactgg
     2521 ctaaattcat cttcttccat gataatttga ttcgaacttt gtatcgggta atcgccacat
     2581 aatcctcctg gcaaatctga cggcacttct ccggctaatc taatcttaaa gtcaatcggt
     2641 gctgcttcgt tatatataat tgaagtgtca gtgtcttcat aggcatttcg tgtagcccca
     2701 acaaatgaaa aagaatattt ttgaccgctc attatggata ccggttgata aattgtttca
     2761 gtgtagcatt catgcggacc aggttgaaaa tgatatgcat tgagacacat atagaaagat
     2821 tcgaactcag gattcgtaac cccatagcag ctggaaatgg tgtagttatc aggt
//
LOCUS       DAOQQY010000325         2867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18326_length_2867_cov_4.528805, whole genome shotgun sequence.
ACCESSION   DAOQQY010000325 DAOQQY010000000
VERSION     DAOQQY010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18326_length_2867_cov_4.528805"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            166..951
                     /locus_tag="PLE23_11880"
     CDS             166..951
                     /locus_tag="PLE23_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMX84008.1"
                     /translation="MNLSLLGKNALVCGGSRGIGKASAIELALLGANVTLLARTESAL
                     QAAVSELDCTQGQIHDYLVVDSQNEAEIREIIGNIVSKRHYNILVNNTGGPPAGAVTE
                     ALPQQFELVFRHHLVANHVISTLLIPGMKASGYGRIINIISTSVKQPLENLGVSNTVR
                     GAVANWSKTLANEVAQFGITVNNVLPGATRTERLEEIIMNNAAKSGKTPDEVAAQMQS
                     AVPMKRIGEAQEIAAAVAFLASPAASYITGINVPVDGGRTKCL"
     gene            complement(993..2231)
                     /locus_tag="PLE23_11885"
     CDS             complement(993..2231)
                     /locus_tag="PLE23_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002661201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMX84009.1"
                     /translation="MSIFELLRQAFTNVRTNLLRSVLTMLIIAFGIMALVGILTSIDS
                     TIYSLSDSFAGMGANSFEIEPKTNEVQGRRFGRRQKIADPVSVKQAQSFKERYEFPAK
                     VSITYPGSRSAQVKFETKKTDPNVEISGIDENYLDVKGYDLSAGRNFTKHEIASGNFK
                     AIITDGIVKKLFRGNPEKALEQSVIVNGHRFRVIGVLKSKGSAMNFSGDRSVLIPFEN
                     ARINFSSPNLNIPIIVKVLRPDAMQDAESAAIGLFRNIRMLKIKDENNFEIQKSDALV
                     DELKDNTTKLRMSAVVIGMITLLGAAIGLMNIMLVSVTERTREIGIIKALGAKRSNIM
                     FQFLTEAIIICQLGGMVGIILGILVGNIITLFMGGSFLIPWVWITLGVITCLIVGIAS
                     GMYPAIKASKLDPVEALRYE"
     gene            2389..>2867
                     /locus_tag="PLE23_11890"
     CDS             2389..>2867
                     /locus_tag="PLE23_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HMX84010.1"
                     /translation="MLSFIEENYIKSIYKIAEASGGPVSTNSLAEKMGIKAATVTDML
                     RKLSDKSFIHYQKYQGVTLSEKGNNRALQIIRKHRLWEMFLVQKLNFGWDEVHEVAEQ
                     LEHIQSEKLIHELDRFLDYPTIDPHGEPIPTASLEMNREPVWYLFNFRKADILTVVGV
                     "
BASE COUNT          864 a          549 c          624 g          830 t
ORIGIN      
        1 caatccattt ttttagatta aaaggttttg caatgctcat attgaactgt atttttggtt
       61 gaagtcaaaa tattcttgta agacaaattt actatttaat tttataaacc taaggtagca
      121 ggtatcaatt ctttatattt gtgtagatat ataaattaaa acgcgatgaa tctatcttta
      181 ttgggtaaaa atgcacttgt ttgcggcggt agccgtggta taggaaaagc ttcggccatt
      241 gagctggcgc tgcttggagc aaatgttacc ttactggcca ggacagaaag tgcacttcag
      301 gcggcagtgt cagaacttga ttgcacgcag ggtcagatac atgattacct ggtggttgat
      361 agccagaatg aagccgaaat aagagaaata ataggaaaca tagtcagtaa gcgtcactac
      421 aatattctag tgaacaatac gggtggtcca ccggccggtg ctgtgacgga ggcattgcca
      481 caacagtttg agctggtatt caggcaccat ttagtagcta atcatgtgat ttctacattg
      541 ttgattcccg ggatgaaagc aagtggatat ggccggatta tcaacatcat ttctacaagt
      601 gtaaagcagc cacttgaaaa tcttggagtg tcgaataccg tacggggggc agtagccaat
      661 tggtcaaaaa cccttgccaa tgaagtggca caatttggca ttacggtaaa taatgtacta
      721 ccgggagcta ctcgtacgga aaggcttgaa gagataataa tgaataatgc tgccaaatcg
      781 ggcaaaacgc ctgatgaagt agctgcacaa atgcaaagtg ctgtaccgat gaagcgtatt
      841 ggtgaagcac aagagatagc tgctgctgta gcttttctgg ccagcccggc agcatcttat
      901 atcacaggca taaatgtacc ggtagatgga ggacgcacca agtgcctgta agtaaggcca
      961 ataaaacaat ggtttcaggt agtaagaaag tgttattcgt atcgcagtgc ctcaaccggg
     1021 tcgagtttgg atgctttgat ggcaggatac atacccgaag cgataccaac tatgagacaa
     1081 gtaatgacac cgagtgttat ccatacccaa ggaatgagaa agctgcctcc catgaatagt
     1141 gtgataatat taccaaccag aatgcctaag ataataccca ccatgccacc caactgacaa
     1201 ataataatag cctcagtcaa aaactgaaac attatattcg aacgtttggc tccgagtgct
     1261 ttgatgatgc ctatttcgcg tgtgcgttct gtaacgctta cgagcatgat attcatcagg
     1321 ccaatggctg cacccagcaa tgtaatcata cctattacta cggcagacat tctgagtttg
     1381 gtggtattgt ccttcaattc atcaacaagt gcatcacttt tttgtatttc aaaattgttt
     1441 tcgtctttta tcttgagcat tcttatattc ctgaaaagcc cgatagctgc tgattcggca
     1501 tcttgcatgg cgtcaggtcg caatactttt actatgatgg gaatgttcag atttggagaa
     1561 ctgaaattga tacgggcatt ctcaaaaggt atcaaaacag agcggtcgcc actgaagttc
     1621 atggcagaac ctttgctttt gagtacacct atgaccctaa agcggtggcc gttgacaatg
     1681 acagattgct cgagtgcttt ttcggggttg cccctgaaca attttttaac gataccgtcc
     1741 gtgatgattg ctttaaaatt gccggaggcg atttcgtgtt tagtaaaatt cctgccggcc
     1801 gataaatcat atcctttgac gtcgagataa ttttcgtcaa ttccgcttat ttctacattc
     1861 gggtctgtct tttttgtttc aaattttact tgtgcacttc gcgatcccgg atatgtgatt
     1921 gaaaccttag ccgggaattc gtaacgttct ttgaaactct gtgcttgttt tacggagaca
     1981 gggtcagcta ttttttgtct tctgccaaat cgtctgcctt gtacttcatt ggtctttggt
     2041 tcgatttcga acgaattggc gcccatgccg gcaaaactgt cgctaagaga atatatagtg
     2101 ctgtcaatag atgtaaggat gcctaccagc gccataatgc caaatgctat gatgagcata
     2161 gtaagtacag acctcagcag gttggttctt acattagtaa aagcctgacg tagtaattca
     2221 aaaattgaca actttactga ttttcaacaa atttacaata tcaataaata ttacatttca
     2281 tatttcgatt aacctttgat ttaaatagat ataaaaattt ccataaaaat ttaaataaaa
     2341 tttagactaa tctaaataaa tatttatatt tgcataaaat tggaaaaaat gttgagtttc
     2401 atcgaagaga attacatcaa atcgatatat aaaattgcgg aagccagtgg tggtccggta
     2461 agtactaatt cgttggccga aaaaatggga attaaggctg caaccgttac tgatatgctg
     2521 cgaaagctaa gtgacaagtc attcattcat tatcagaaat accagggtgt caccttgtcg
     2581 gagaaaggta ataaccgtgc attgcagatc atccgcaagc acagattgtg ggaaatgttt
     2641 ttggtacaaa agcttaattt cgggtgggac gaagtgcacg aagtagcaga gcagctcgaa
     2701 catatacagt cagaaaaact gatacacgaa ctagatcgtt ttcttgatta ccctaccatt
     2761 gacccacatg gagagcccat tcctactgca tcgctggaaa tgaatcgtga accggtatgg
     2821 tatctgttca acttcagaaa agctgatata ctgacagtag tgggcgt
//
LOCUS       DAOQQY010000326         2863 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18357_length_2863_cov_5.083333, whole genome shotgun sequence.
ACCESSION   DAOQQY010000326 DAOQQY010000000
VERSION     DAOQQY010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18357_length_2863_cov_5.083333"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1807
                     /locus_tag="PLE23_11895"
     CDS             284..1807
                     /locus_tag="PLE23_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMX84011.1"
                     /translation="MSNAGFENYAFILVLAYAFVILFFVIRGAMKTKSIQDYALGSRG
                     FSPLVLGLSLAAGITSAATFIINPGFVAFFGWSAFLSMSLILPVGLYGSLIYLSKSFT
                     HYGQSVKAATLSQWIGLRFGSKAYGQLMAFFSLLLITFIVLICVGLTKVLAQALKIDE
                     FTALVGMVIFVFGYMMFGGANAMIYTNAIQAVIMLVVAIILLGSGAHFFEGGISGFWE
                     KIASIDPNLTKNFNQTSPLFRDWFEVIFCNFVIGIAIVCQPHIITRSLMLEKGASLNK
                     FLLYTIVVETIFFFVLFVGFYARIEFPDLTADGQAIKMDGIMSMYVVKNFSNFTGLII
                     IVGLISAGLSTLEGLIQSVSTTITSDLIRPMLYGDKSDDETLQRKLKNVNRIVIIVLA
                     VIAIFWSNQQLANPKLSVGILAQNGVYAFFAAAFVPVLQGIFLKNVPRIAPMAASITA
                     IIVHFSVYYGQLTPYTTGEVRNPAVAGTIAIIAACIVGFGLYFIKKNDLSLQPKTDI"
     gene            1807..2796
                     /locus_tag="PLE23_11900"
     CDS             1807..2796
                     /locus_tag="PLE23_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HMX84012.1"
                     /translation="MRNAIIIGSGSYAPEQIVPNSFFNSILGTDVDSWLREVVQISER
                     RWCQDEESVADLIEKACQAAMDDAGITAGMVDLLIIGTDTPEYISPATASVIQHRMKM
                     INAATFDVNAACAGFVTALDIGARYIRTDEDYKHVMVVGAYTMSKHLDLTDKKTVTLF
                     ADGAAAIVLAAEDKPGKGFLAGKLQADGQYCEAMGIYGGGSKYPVTHDLIDAKKHKLQ
                     FVKKIPGEVNPDVWTKLVHDICHKANIAVTDIDHYFFTQININSIFETMDRLGQPREK
                     AATIMHHFGYTGSACLPMAFDQWKKSGKLRPGDTVLFVGSGGGLAFGVCLFKL"
BASE COUNT          798 a          550 c          637 g          878 t
ORIGIN      
        1 ttacattaat gcgactaata aacaagacgg tacggtcaaa acaggaaaat tgtccattgt
       61 cagatagggt gatattgtac attgctcttt agtcatatta gaccgatttt acattttcgg
      121 tcaaccctat atatttttgt attaatatag tgataaatag tattgtacgt aaaaataatt
      181 tttatagtca acctgtaaaa ttgcattgtt gattactttg ccggagattt aagaattata
      241 ttcaccaaaa attactatta actactaacc aatcagtctg atcatgtcaa atgccggatt
      301 tgaaaactat gcgtttatat tagtattagc ttacgcattc gttatattgt ttttcgtcat
      361 tcggggtgcg atgaagacca agtccattca ggattatgcg ctcggcagca gaggtttttc
      421 acccttggta ttggggcttt cacttgccgc aggcatcact agtgctgcta cattcatcat
      481 caatccaggg ttcgtggcat tctttggctg gagcgctttt ttgagtatgt cgctgatatt
      541 gccggttggt ctgtatggtt cgctcatcta cctctctaaa agctttacgc attacggtca
      601 gtcagtaaag gcagctactt tgtcgcaatg gattggccta cgttttggaa gcaaagctta
      661 tggtcagctt atggccttct tctcattgct gttgattacg tttattgtgc ttatttgtgt
      721 cgggcttaca aaagtattgg ctcaggcgct taagattgat gaatttactg ctcttgttgg
      781 tatggtaatc ttcgtattcg gctacatgat gttcggtggc gcaaatgcga tgatttatac
      841 aaatgccata caagctgtaa tcatgttggt ggtagctatc atattgctag ggtccggtgc
      901 acattttttt gaaggaggca tttcggggtt ttgggaaaaa atcgcaagca tcgaccccaa
      961 cttgacaaag aattttaatc aaacaagtcc gttgttcaga gactggtttg aggtgatatt
     1021 ctgcaatttt gttataggta tagccatcgt atgtcagcca cacatcatta ccagatcgct
     1081 catgcttgag aagggcgctt cgctcaataa attcctgctc tacactattg ttgtggaaac
     1141 catcttcttt tttgttcttt ttgtaggctt ttatgccaga attgaatttc cggatcttac
     1201 cgccgacggg caagctataa agatggacgg tattatgtca atgtatgtag taaaaaattt
     1261 ttcaaacttt acgggcctga ttataattgt cggccttatc tcagcaggat tgtcaacgct
     1321 tgaaggtctg attcagtcag tatctactac cattacatca gacctcatca gaccgatgtt
     1381 gtatggtgat aaatcggatg atgaaacatt gcaaagaaaa ctcaaaaacg taaaccgaat
     1441 tgtcataata gttttggctg tgatagccat tttttggagc aatcaacaat tagccaatcc
     1501 gaaactcagt gtcggtatat tggctcaaaa tggtgtctat gcttttttcg cagcggcttt
     1561 cgtccctgta ttacagggaa tttttctcaa gaatgtgcct cgtatagcac ctatggcagc
     1621 ttccatcaca gcaataatcg ttcatttttc ggtttattat ggtcagctta ctccttatac
     1681 caccggcgag gtcaggaatc ctgccgttgc aggtaccatc gccattatag ctgcatgtat
     1741 tgttggcttc gggttgtatt ttattaaaaa gaatgactta tcgttacaac ccaaaactga
     1801 tatataatga ggaatgccat catcatcgga tcgggttcgt atgcgccgga acaaattgtg
     1861 ccgaattcat tttttaattc catcctgggt acagatgttg attcgtggct tcgtgaagtc
     1921 gttcagatat ctgaacgaag atggtgccaa gacgaagaaa gtgttgcaga tctcatcgaa
     1981 aaggcatgcc aggcggcaat ggatgatgcc ggcataacag ccggaatggt tgatttgctc
     2041 ataataggta cagatacacc tgagtatatt tcgccggcta cggcatctgt catacagcat
     2101 agaatgaaaa tgataaacgc agctacattt gatgttaatg ctgcctgtgc agggttcgtt
     2161 acagcactcg acatcggtgc cagatacatc cgtactgatg aagattataa acacgtaatg
     2221 gtagtgggtg catatacaat gagcaaacac cttgacctta ccgacaagaa gaccgtaacg
     2281 ctttttgctg atggtgctgc tgccatcgtt ctcgcagctg aagataaacc gggcaaagga
     2341 ttcctggcag gaaaattaca ggcagacggc cagtattgcg aagccatggg catctatggt
     2401 ggaggctcaa agtatccggt gacacatgac ctgattgatg ctaaaaaaca taagctacaa
     2461 tttgtcaaaa aaataccggg cgaagtaaat cctgatgtat ggactaaatt ggtacatgat
     2521 atttgtcata aagcaaatat agcggtgact gacatagatc attatttttt tacacaaatc
     2581 aacataaatt cgatttttga aacgatggac agactcggac aaccacgaga gaaagcagct
     2641 accatcatgc accatttcgg atataccggt tcggcgtgcc tacctatggc attcgaccaa
     2701 tggaagaaaa gtggaaagct gcgaccgggc gatacggtgc tctttgtagg ctcaggaggt
     2761 ggcttggcat tcggagtatg tttgtttaaa ttgtaatgaa tgattatgga taatatgtgg
     2821 caacaagact gggtatcgaa atgggctttg tacagccccg aaa
//
LOCUS       DAOQQY010000327         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18458_length_2855_cov_5.066429, whole genome shotgun sequence.
ACCESSION   DAOQQY010000327 DAOQQY010000000
VERSION     DAOQQY010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18458_length_2855_cov_5.066429"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="PLE23_11905"
     CDS             complement(<1..1050)
                     /locus_tag="PLE23_11905"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMX84013.1"
                     /translation="MKKFTWFFILLLWVILPAYSDAQTSGISGKITDSVTGEMLADAT
                     IRTANAGTQSDEKGEYKLNLAPGSYDVQFSYIGFVQKTIRITINEGELKNLNVSLYEN
                     ATILQTTTVTAGKYETPLAEATVSLEVIKPSLIENTGSTTIDKVLDKIPSINIIDGQV
                     NIRGGSGFSYGAGSRVLLLIDDLPALQADAGYTRWSDVPVENIEQVEVLKGAASALYG
                     SAAMNGIINIRTGYARSVPETKAAIYARTVTSPADASKKWWHTPPVEYGAQILHKQRF
                     NKLDVILGGFYSGGDQFIKDTYDRYFRINTGLRYRINNRLSIGINLLLNDGESQSYFL
                     WQNKDAGAWIGDTNTL"
     gene            1174..1995
                     /locus_tag="PLE23_11910"
     CDS             1174..1995
                     /locus_tag="PLE23_11910"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84014.1"
                     /translation="MRSVLLALSFLFSVALAHAQFGQCTPDPKYNGAAVGVYPLPYDP
                     ATNPDGGINTSACKSFPYQFNFNVVVPDTINFPGFGDIPLESLSLATTGAFSNLPAGI
                     SYSCNPPNCIFLKNTVGCVALYGTVPTSVATGDYELIINGTIGTGLGISLPFTFPNAS
                     LYPGSYILKVEPEDGKTCYKVSTSEVRSKSYYGEINPNPVSMEANLTIFSQKTTQLKL
                     EILSIDGKMMYSKKMFVQEGQNIEKIDVSNFANGVYMYRIADDIETALGKMVIQN"
     gene            complement(2062..2403)
                     /locus_tag="PLE23_11915"
     CDS             complement(2062..2403)
                     /locus_tag="PLE23_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HMX84015.1"
                     /translation="MKKEKITPADFDKIEMRTGTILTASVFTKAHKPAYILTIDFGEL
                     GILKSSAQISDLYNVDDLPGRQIIAVTNFPPKQIADIMSECLVLGVVNADNKVVLLTP
                     DRQIPNGLLIS"
     gene            2529..>2855
                     /locus_tag="PLE23_11920"
     CDS             2529..>2855
                     /locus_tag="PLE23_11920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMX84016.1"
                     /translation="MTVQEYNISVDEYADAIYRFAYKMTKSKSDADDLVQVSFEKLWR
                     NYESVEFVKVKSWLFTTIYRSFIDSKRKIKLELVADIEDGGHYEGNTSFELKEMLNKA
                     LDLLPEI"
BASE COUNT          824 a          563 c          576 g          892 t
ORIGIN      
        1 caatgtattg gtgtcaccta tccaggcgcc tgcatcctta ttttgccata ggaaatagct
       61 ttgtgactct ccgtcattga gtaaaagatt aatacctata ctgagtctgt tgttgatacg
      121 gtatctgagc ccggtattaa ttctaaaata tctgtcataa gtatctttga taaactgatc
      181 gcctcccgag tagaaaccgc ccaagattac atccagtttg ttgaaacgct gcttgtgaag
      241 tatctgagcg ccgtattcta caggtggtgt gtgccaccat tttttactgg catcagcagg
      301 gctcgtaacg gtgcgtgcat atatggctgc cttagtttcc gggacagaac gcgcatatcc
      361 ggtacgtata tttatgatgc cattcatagc agctgagcca tacaaggcag aagctgcacc
      421 ttttaatact tctacctgtt cgatattttc aaccggtacg tctgaccatc gcgtatagcc
      481 agcatcggcc tgcaaggcag gtaaatcatc aatcagcaga agcactctac taccggcacc
      541 ataagaaaag cccgatccgc cacgtatatt tacctggccg tcaatgatgt ttatgcttgg
      601 tatcttgtca agtactttgt ctattgtcgt agagcctgta ttttcaataa gggaaggttt
      661 tattacttca agcgaaactg ttgcttcagc aagtggagtt tcatatttgc ccgcagtgac
      721 agtggttgtt tgtaatattg tcgcattttc atatagacta acattgaggt ttttcaattc
      781 gccttcattg atggtaatgc ggatggtctt ttgaacaaaa cctatgtaac taaactgtac
      841 atcgtagctt cccggagcga ggttaagttt atattcacct ttttcgtcag attgtgtacc
      901 ggcattggca gtacgaatag tagcatcggc aagcatttca cctgtaacac tatccgttat
      961 tttgcccgaa atacctgaag tctgagcgtc ggaatacgcc ggcaaaatta cccatagcag
     1021 gagtatgaaa aaccatgtaa attttttcat aagtatttgt ttaatcagct gttttaaaat
     1081 atttgaaaca attttaatgc ttttttatga aactatgaca gactttaatt aaatttatgt
     1141 tcagattcaa taattaacca aaaaatatag tttatgagat cagtattact tgctttgtca
     1201 tttttgtttt cagtagctct tgcacacgca cagtttggcc aatgtacacc tgacccaaag
     1261 tataatggtg ctgcagtagg tgtttatccg cttccttatg atcctgccac gaatccggat
     1321 ggtggtatca atacaagtgc ctgcaagtca tttccttatc aattcaactt caatgttgtg
     1381 gttccggata ccattaattt tcctggcttt ggcgatattc ctcttgaaag tctgtcgctg
     1441 gctacaacag gtgccttttc taatcttccg gctggcatta gttattcctg caatccgcca
     1501 aactgtattt ttctgaaaaa tacagtaggc tgtgttgcgc tgtatggtac cgtacctacc
     1561 tcagttgcca caggtgatta cgaactcatc attaatggta ctattggtac cggcttaggt
     1621 atcagtctgc cgtttacttt cccaaatgcc tcattgtatc cgggtagcta tattcttaaa
     1681 gtagaacccg aagacggaaa aacctgctac aaggtctcta catcagaagt gaggtcgaag
     1741 agctattatg gtgaaatcaa tcctaatccg gtaagtatgg aagctaattt gaccattttt
     1801 tctcaaaaga caacacaact caaactcgaa atcctttcta ttgatggcaa gatgatgtac
     1861 tcaaagaaaa tgttcgttca ggaaggtcaa aatattgaaa aaatagatgt cagtaatttt
     1921 gcaaatggtg tctatatgta ccgaatcgct gacgacattg aaactgcact tggtaaaatg
     1981 gtcattcaga actaagattg ttcttggatg aatagcaaag atgcttccgg atatgatttc
     2041 ggaagcatct tttttatttt atcaggatat cagcaatccg tttggtatct ggcggtcggg
     2101 tgtgagtaac acgaccttgt tgtcagcatt cactacaccc aatacgaggc actcgctcat
     2161 gatatctgca atttgttttg gcgggaaatt ggtaacggct attatctgtc gcccgggaag
     2221 atcatcaaca ttgtacaaat cagatatttg ggcagaagat ttcaatatac caagttcgcc
     2281 aaagtctatt gtcagaatat aagctggctt gtgggctttg gtgaatacgg atgcagtgag
     2341 gatggtgcct gtgcgcattt ctattttatc aaaatctgcc ggagtaattt tttccttttt
     2401 cataaaattc ttgttcagaa cgccaaaata ctcagaatcc tcagaatcct gcagtatttt
     2461 taatgtgcaa tttttttttt tcaatagcct tgtaactaat tacacaatag aggcgtcata
     2521 caagcgagat gacagtacaa gaatacaata tcagtgttga tgaatatgca gatgctatct
     2581 acaggtttgc atacaaaatg acaaagtcaa aatcagatgc agatgatctt gtacaggtaa
     2641 gcttcgaaaa actctggcgc aactacgaaa gtgtagaatt tgtaaaagtt aaatcatggc
     2701 tttttacaac aatttaccgg agttttattg actcgaaaag gaaaataaaa ctcgaactgg
     2761 tagcagatat agaagatggc ggacactacg aaggcaatac cagttttgaa ctaaaagaaa
     2821 tgctaaataa agctcttgac ctgctgccgg aaatt
//
LOCUS       DAOQQY010000328         2851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18501_length_2851_cov_5.317239, whole genome shotgun sequence.
ACCESSION   DAOQQY010000328 DAOQQY010000000
VERSION     DAOQQY010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18501_length_2851_cov_5.317239"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="PLE23_11925"
     CDS             <1..1136
                     /locus_tag="PLE23_11925"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84017.1"
                     /translation="PLLTDQMIDLGTGTHPAFIDIDNDGDMDMIAGTFNSFTNLTERN
                     GHLYLYENTGTADQAVFELVDSDFLGFSAFSNLDWGFAPTVGDLDGDGDVDILTGSED
                     GILYYNENTAGVGNVPVFAATVVKYKDIDVGQAASPFIFDVDDDGLPDLLIGERNGNI
                     NFLPNQGSLLNPQFEANVDKAPNINYFGKVDTRQPGYASGYSSPVLFRINDKLHLLTG
                     SLSGGIQLYNDIENNFTGKFTTVTKQLGNINEGERTKPVLTKLIKGDYYNMVVGNMRG
                     GFGVFGTNISTKTTAVKNLLTQASIRLMPNPVEAYINIMSPDITDLRSFDASIFDMQG
                     RQWYSMSIIPNIQQIDVSALPSGMYFIHLRSLTDSRVLKFVKN"
     gene            complement(1143..2477)
                     /gene="ffh"
                     /locus_tag="PLE23_11930"
     CDS             complement(1143..2477)
                     /gene="ffh"
                     /locus_tag="PLE23_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HMX84018.1"
                     /translation="MFNNLSDKLDLAFKTIKGDSRLTEINVAESIKEIRRALVASDVN
                     YKIAKEFTDKIKDEALGTQNILKSVKPGELMVKIVMDELTELMGGQSAGINIKGAPAV
                     VLIAGLQGSGKTTFSGKLAYYLKNKKSKKPLLVACDVYRPAAIDQITVLGEQTHVTVY
                     KEPENKNPLEIAQNAVKHAKANGYDVVIVDTAGRLSIDEDMMAEIESLKNNLQPQETL
                     FVVDSMTGQDAVNTAKNFNDRINYDGVVLTKLDGDTRGGAALSIKYTVGKPIKFVSMG
                     EKMETLDVFYPDRMAQRILGMGDIVSLVEKAQSEFDEKEAKKIEAKIKKNKFDFNDFM
                     VQLNQIKKMGDLKSLMGMIPGMSKITKDLDIDNSAFSKVEAIIQSMTIEERGNPDLLN
                     MSRKNRIARGCGKSIHEVNQFIKQFEQMKKMMHTFSKGQGMRGFNPNMLGKN"
BASE COUNT          768 a          541 c          591 g          951 t
ORIGIN      
        1 aacctttgct cactgaccag atgatagact taggcacagg tacgcatcct gcctttatag
       61 atatagacaa tgatggtgat atggatatga tagccggtac cttcaattcg tttacaaacc
      121 tgacagagag aaatggtcat ttataccttt atgaaaacac agggactgca gatcaggcgg
      181 ttttcgaact tgtggacagt gattttcttg gattttcggc attcagtaat ttagattggg
      241 gttttgcacc aacagtaggt gacttggacg gtgacggcga tgttgatatt ctgaccggca
      301 gtgaagatgg tatattatat tataatgaga atacagccgg agtagggaat gtaccggttt
      361 ttgcagctac agttgtaaaa tacaaagata ttgatgtagg ccaagcggca agtccgttta
      421 tttttgatgt agatgatgat ggtctgccag atttgctcat tggtgaacga aacggaaata
      481 tcaatttttt gcccaaccag ggaagtttgc tgaatcctca gtttgaagca aatgtcgata
      541 aggcaccgaa tattaattat tttggaaagg tggacacaag gcagccaggc tatgcgagtg
      601 gatattcgtc gccggtattg tttagaatca acgataaact gcatctgttg acgggttcac
      661 tcagtggagg tattcaactt tacaatgata tagagaataa ttttaccgga aaattcacga
      721 cagtaacaaa gcaacttggc aacatcaatg aaggtgaaag gacaaaacca gtattgacca
      781 aactgataaa gggcgattat tacaatatgg ttgttggcaa catgcgtggt ggattcggag
      841 tatttggcac caatatttct accaaaacta ctgctgttaa aaatctgcta acccaggcaa
      901 gtatcagact aatgccaaat ccggtagaag cgtacatcaa tattatgtca cctgatatca
      961 ccgacctacg ttcatttgat gcttcaatat ttgatatgca aggtaggcaa tggtactcga
     1021 tgagcatcat acctaatatt caacagatag atgtgtcggc attgccatcg ggtatgtatt
     1081 ttatacatct gcgttcatta acagactcgc gggtattgaa gtttgtaaaa aattgaaaaa
     1141 aattaatttt ttcctaacat gtttgggtta aatccgcgca ttccctggcc tttgctgaag
     1201 gtgtgcatca tttttttcat ttgttcaaac tgtttgataa actggttgac ttcatggatg
     1261 cttttgccgc aacctcttgc aatacggttt tttctgctca tattgagtaa gtccggattg
     1321 ccgcgctctt caatcgtcat ggattggatg atggcttcta cctttgaaaa agctgagttg
     1381 tcaatatcca ggtctttggt tattttgctc atgcccggta tcattcccat gagtgatttg
     1441 aggtcaccca tttttttaat ctgattaagc tgaaccataa agtcgttgaa gtcaaatttg
     1501 tttttcttga ttttggcttc aattttttta gcttcttttt cgtcaaactc agattgggct
     1561 ttttctacaa gtgaaacaat gtcacccatg ccgagaatac gttgtgccat acggtcagga
     1621 tagaatacat cgagtgtttc catcttttcg cccatggaca caaattttat aggtttgcct
     1681 accgtgtatt ttatagacaa ggcagcacca ccgcgtgtgt cgccatcgag ctttgtaagc
     1741 acaacaccgt cataattgat acgatcgttg aagtttttgg cagtattcac tgcgtcttgt
     1801 ccggtcatgg agtcaacgac gaaaagtgtc tcttgtggtt gaagattgtt cttgagtgat
     1861 tcgatctctg ccatcatgtc ttcatctata gagagacggc cggcagtatc cactattact
     1921 acatcatatc catttgcttt tgcgtgtttt acagcattct gggctatttc gagtgggttc
     1981 ttgttttcgg gttctttata aactgttaca tgagtctgtt ctccaagtac ggttatctgg
     2041 tcaatagctg caggacgata aacatcgcat gctacgagta agggtttctt tgattttttg
     2101 ttttttaggt aataggccag ttttccggag aatgtcgtct ttccactacc ttgaagccct
     2161 gcgataagta ctacagcagg tgcaccttta atattgatac ctgcagattg gccacccatg
     2221 agttcagtga gttcatccat cactattttt accatgagct cgcccggctt taccgatttc
     2281 aggatgtttt gagtgccgag tgcttcatct ttgattttgt cggtaaattc tttggctatc
     2341 ttgtagttaa cgtcagatgc cacaagggca cgtctgattt cctttattga ttctgcaacg
     2401 ttgatttctg taagacggct gtcaccttta atggtcttaa acgccaggtc taatttgtcg
     2461 gaaagattat tgaacatgaa tggatatgaa ttttagcctg caaagttaat gctttttatg
     2521 aaatatttta actcatataa cgcattaggc tttttactgc gaataattat tagttcaaat
     2581 ccattgctaa agctctgaaa ccatagttca ctatactttt atctctcttt tctggattat
     2641 ttgcaataat cattttcgtt taaatcccta atgcgttacc ggggtttgat aaatctacta
     2701 cttaatggtt caaaaaatgc tgaaaaaaat gtgtcacaac atctcacttt tcacttttca
     2761 cttttcactt ttcacttttc acatctcact tttcactttt aatttttaat tgaatgcgcg
     2821 tgaaggatag aagccggcac aacgtaaggc g
//
LOCUS       DAOQQY010000329         2836 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18647_length_2836_cov_5.110752, whole genome shotgun sequence.
ACCESSION   DAOQQY010000329 DAOQQY010000000
VERSION     DAOQQY010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18647_length_2836_cov_5.110752"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..680
                     /locus_tag="PLE23_11935"
     CDS             12..680
                     /locus_tag="PLE23_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84019.1"
                     /translation="MEIFKNLKSIFIVEAGADKATEPGSTETPATTNENTNNSPTAPP
                     TVSVSEGKPEDRFIKVLYEAIKKQGKDGFDYLEFQESLESLEKLSMDEQTRYQSAFAV
                     AKTMGATPASLIEAANYYMSVLHNEEKAFEETLKSQMQLQVGDKQSEKQKLQEKIVAQ
                     NAQIAALQKEISQHQSQLDNIDKQISDASTKIETTKNNFIASYNQLVDKITQDIEKMK
                     QYLK"
     gene            687..1709
                     /locus_tag="PLE23_11940"
     CDS             687..1709
                     /locus_tag="PLE23_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84020.1"
                     /translation="MMDESFKPKSFWQRPEGITGMLVAGGIIVGIGYILYNFLPVLIN
                     LAENTLYLSGMLIALAALIYMVIDPRLRNLVYYGYKSIMRFITGLFIDLDPIGILKSY
                     VEDLEKNLYKMSQQIGKLRGQMHKLNEIIFNNRKEINDNLSLANKAKASNKNSMVILK
                     TRKAGRLQESNLRLEDLYKKMEIMYRVLSKMYENSEILKEDIADQVEVKQQEREAILA
                     SHSAMKSAMSVISGDPDKKAMFDMALEAIADDVSKKVGEMERFMDLSSNFMDSIDLNN
                     GIFEEEGLKMLEKWEKEGASLLLGGDKDDILRKSNDDNDVLDLNTPVRLPEKVNRTNQ
                     YDDLFK"
     gene            1730..>2836
                     /locus_tag="PLE23_11945"
     CDS             1730..>2836
                     /locus_tag="PLE23_11945"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMX84021.1"
                     /translation="MQTRLTTFSKILITALILGVIFFGFKYFLNNTNMGKDLKKNSEN
                     IDNSSGTSILGGGKKDDDVITVQMFTWGGYVPGLYFNEGAQASTNSRFYKDYGIKVEF
                     KLIDDFDASRNAWKSGDVDLLGNEVSAMATEMTGLGPYQPQIVLQCDWSRGGDAIVAK
                     RGINSFNDLKGKVVAVTPSTPSMTFLLWMLEAANLKLSDLKQVKEVPSAIDAATAFKS
                     GQVDAAVVWSPDDEIIVREVPGSKVLQSTRQASNIIADVYMAKKSYVDKNADKLAKFY
                     EGWMKAAAEINTSAGARQKAAKIMAQVTGISEQDAQASINNVRLTTHGDNLNFFGKNP
                     NYKGITGEGLYTKMGNIYQSMGLAPADRPNWRLMS"
BASE COUNT         1016 a          548 c          577 g          695 t
ORIGIN      
        1 attaagacct gatggaaata tttaaaaatc ttaagtctat attcattgtc gaagcaggtg
       61 ctgacaaagc tactgaaccg ggtagtacgg aaacgccagc cacaaccaat gaaaatacaa
      121 ataattctcc aactgctcct ccaacagtat ccgtatcaga aggaaaaccg gaagatagat
      181 ttataaaagt attatacgaa gcaataaaaa aacaaggaaa agatggtttc gattatctcg
      241 agtttcaaga atcactcgaa tcactcgaaa aattatcaat ggatgagcag accaggtatc
      301 aatcggcatt tgctgtcgcc aaaacgatgg gagccacacc tgcttcatta atcgaagcag
      361 ccaattatta tatgagtgtg ctccacaatg aagaaaaggc attcgaagaa acactcaagt
      421 cgcaaatgca actgcaagta ggtgacaagc aatccgaaaa acaaaagtta caagaaaaaa
      481 ttgttgccca aaatgctcaa atcgcagcct tacaaaaaga aatatcacaa caccaaagcc
      541 agctcgacaa catcgacaag caaataagtg atgcttctac aaagatagaa actactaaga
      601 ataattttat cgccagctac aatcaacttg tagataagat aacccaagat attgaaaaga
      661 tgaaacaata ccttaaataa tattgcatga tggacgaaag ttttaaaccg aaatcattct
      721 ggcaaagacc agaaggaatc acaggaatgc ttgtagcagg tgggataata gtcggcatcg
      781 gttacatttt atacaatttt ttgcctgtat taatcaatct tgctgaaaat acgctttacc
      841 tatccggaat gctaatagca cttgcggcac ttatctatat ggtcatcgat ccacgtctga
      901 ggaatcttgt gtactacgga tacaaaagta tcatgcgttt tattaccggt ttatttattg
      961 atcttgaccc aatcggcatc ctgaaatcgt atgttgaaga tctggagaaa aatctttata
     1021 aaatgtcaca acagataggc aaactccggg gccagatgca taagctcaac gaaataattt
     1081 ttaacaaccg caaagaaatc aacgataacc tgtcacttgc caataaagcg aaagcctcga
     1141 acaaaaattc gatggtgata ctcaaaaccc gtaaagcagg tcgccttcag gaatcaaatc
     1201 tccgtctgga agacttgtat aaaaaaatgg aaatcatgta tcgcgtactt agtaaaatgt
     1261 acgaaaattc ggaaatacta aaagaggata ttgcagatca ggtagaagta aaacaacaag
     1321 aacgcgaagc aattttggca tcacattctg ctatgaagtc tgccatgagt gtcatctcag
     1381 gtgaccctga caaaaaagca atgttcgata tggctcttga agctattgca gatgatgtca
     1441 gtaaaaaagt aggcgaaatg gagcgattta tggatttatc aagtaacttc atggattcaa
     1501 ttgacctcaa taacggtata tttgaagaag aaggtctcaa aatgcttgaa aaatgggaaa
     1561 aagaaggtgc atccttgttg ctcggtggtg ataaagacga cattctccga aaatctaacg
     1621 atgataatga tgtactcgac ctgaacacac ctgtcagatt gccggaaaaa gtaaaccgga
     1681 caaaccaata tgatgattta ttcaaataaa aattaaaaaa ttcagaaata tgcaaacacg
     1741 tttgaccaca ttttcaaaaa tactcattac cgccctcatt ttgggtgtaa tattctttgg
     1801 gttcaagtat tttctcaata atacaaacat gggaaaagat ctgaagaaga acagtgaaaa
     1861 catagataat tcatcgggca cttctatact gggtggcggt aaaaaggatg atgatgtcat
     1921 cacagtacaa atgtttacct ggggcggata tgttccggga ttgtacttca acgaaggtgc
     1981 acaggcttca accaacagcc gtttttacaa agattatgga ataaaagtcg aatttaaact
     2041 gatagatgac tttgacgcaa gcagaaatgc ctggaaatcg ggcgatgttg acttgttggg
     2101 caatgaggtg agtgctatgg ctaccgaaat gaccggcctt ggcccttacc agccccaaat
     2161 cgtattgcag tgcgactggt cacggggtgg cgacgctata gtagccaaac gcggtatcaa
     2221 ttcattcaat gacctgaaag gcaaagtggt agcagttaca ccatctactc cttccatgac
     2281 attcttgctt tggatgctcg aagctgccaa cctaaaactg tctgacctga aacaagtaaa
     2341 agaagtacca agtgcaatcg atgccgctac ggcatttaag agtggccagg ttgatgcagc
     2401 agtagtctgg agtccggatg atgaaatcat tgtacgtgaa gtgccgggtt ctaaagtatt
     2461 acaatcaaca cgccaagcgt cgaatatcat tgcagatgta tatatggcaa aaaagtctta
     2521 tgttgataaa aatgccgaca agcttgccaa attctatgaa ggttggatga aggctgctgc
     2581 tgaaatcaat acttcagcag gtgcaagaca aaaagcagca aaaataatgg cccaagtaac
     2641 cggtatttct gaacaggacg cacaagcatc tatcaacaat gtaagactaa ctacacatgg
     2701 cgacaacctc aatttctttg gaaaaaatcc gaactataaa ggaatcacag gtgaagggct
     2761 atatacaaaa atgggtaaca tttaccagtc aatgggatta gctcctgccg accgaccaaa
     2821 ctggcggcta atgtct
//
LOCUS       DAOQQY010000330         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18800_length_2821_cov_4.693420, whole genome shotgun sequence.
ACCESSION   DAOQQY010000330 DAOQQY010000000
VERSION     DAOQQY010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18800_length_2821_cov_4.693420"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2821)
                     /locus_tag="PLE23_11950"
     CDS             complement(<1..>2821)
                     /locus_tag="PLE23_11950"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HMX84022.1"
                     /translation="YYNSSRFLNVMVYSSNIGLIAKSGKAGEVMVIATDLNTTKPLNG
                     VDITFYDYQLQQVKTAKTNADGIAMATGFKVKPFAVVAQQGGRKGYLRLMDNEALNIS
                     QFDVVGEDVQKGVKGFLYGERGVWRPGDSLYLNFMMQSGDQTNIPDGVPVNFEFFDSR
                     GILQSSQVLSSSSDRLYPIYIATRPDAVTGNWKAVLKVGGAVFNFPVKIENIKPNRMR
                     IKLDFGKKELTSSDEPVNCILSANWLYGAPAKNLDARIDVSMWQSKEGFKKFQDFRIN
                     NIREAPFYTTFEWFKGRLNDLGNTKISQKLLPKESNATGPMVVNFKTKVFEQGGEPNV
                     DNVNVTYHPFNRYCGVSVLKNSYGAPTYNVNENVPISLASISTDGNGISQTALKVEIF
                     RVDWSWWWDEDNSNSYSYYKSEMENPIQSYSVKTNAQGKANVQFKSAEWGRYYVRVTD
                     PVSGHTSGEYFYCGYPETEDNYNAIRAASIVPVMSDKENYNVGEEVKLKLECPDKARV
                     LVSLENGSKVVKAMWYDAVKGNNEFKFKTTAEMSPNVYANVSIVQRFGQNVNDMPVRM
                     YGIIPIKVEDKNTRLNPVISMPSELKPSMRSSITINEQSGKAMSYTIDIVDEGLLDLT
                     HFATPDPWKKFFAREALGVKTWDLFDRLAGTFTESSSQILSIGGDAGKAPNPDKQRAI
                     RFKPMVVHLGPFRLEKGKKATHNFTVPNYIGSVRAMVVAIGDGAYGNAEKTIPVRSPL
                     MVLASFPRVIGVGETIKVPVNVFTTNDKVKNVSVTVSDKAGLLQNKSNAQVVSFSKAD
                     EKVVYVDLTAGDREGISKITIHAKGNGETAYQEIEVDIRNPNPVQTTLVSSVVQSGKT
                     ADINLAFNGVKGTNKATLEVSSMLPVNLSKNLQYLISYPHGCIEQITSGAFPQLYVSN
                     FTKLSPKQQSETERIVKSVLNRYKNY"
BASE COUNT          737 a          647 c          546 g          891 t
ORIGIN      
        1 atagttctta taccgattga gaaccgattt cacaatccgt tctgtttcgg actgttgttt
       61 tggcgaaagt tttgtaaagt tggacacata cagttgagga aatgcaccag aagttatctg
      121 ttcaatacaa ccatgcggat aactaatcag gtactgtaaa tttttactaa ggttgactgg
      181 cagcattgaa gaaacctcca gtgttgcttt atttgtacct ttcactccgt tgaatgcgag
      241 gttgatatca gctgtttttc cactctgtac taccgaggac acaagtgttg tctgtaccgg
      301 gttcggattg cgtatatcta cttctatttc ttggtaggca gtttcgccat ttccttttgc
      361 atgtatggta atttttgaaa ttccctccct gtccccggct gtaaggtcaa catacacgac
      421 tttttcatca gcttttgaaa agctcactac ctgtgcattt gacttattct gtaacagccc
      481 tgccttgtcc gagacagtaa cagatacgtt tttcaccttg tcgtttgtcg taaaaacatt
      541 caccggcact ttgatggtct cgccaacacc tatcaccctc ggaaatgaag ccaacaccat
      601 caaaggagat cgtaccggga ttgttttctc cgcattgcca taggcaccgt caccgattgc
      661 cacaaccatt gccctcaccg aaccgatata atttgggaca gtaaagttat gtgttgcctt
      721 tttgcctttt tcgagtcgga acggccctag gtggaccacc ataggtttga agcgtatagc
      781 acgttgttta tcaggatttg gtgccttgcc ggcatcgcca cctatgctga gtatttggct
      841 ggacgactca gtaaaagtac ctgccaagcg gtcgaacaaa tcccaagtct ttacaccaag
      901 tgcttcgcgt gcgaagaatt ttttccaagg atcgggagtg gcgaaatgtg tcaggtcaag
      961 caatccttca tctactatat caatggtgta cgacatagct tttccacttt gttcgtttat
     1021 tgttatgctt gaacgcattg aaggtttgag ttcagacggc atgctgataa cagggttgag
     1081 gcgggtattt ttatcttcca cctttatcgg aatgatacca tacatacgca ccggcatatc
     1141 atttacattc tgcccaaatc gctgtacaat acttacattg gcatacacat taggagacat
     1201 ttctgctgtg gttttgaact taaattcatt attgcctttc acggcatcat accacatagc
     1261 ttttactact ttagatccat tttcgaggct tacgagtacc cgtgccttat ccggacattc
     1321 aagttttagt ttgacctctt cgccaacatt ataattttct ttgtcgctca tgaccggaac
     1381 gatgcttgct gcacggatgg cattatagtt atcttctgtt tcaggataac cacaatagaa
     1441 atattcaccg gaggtgtgcc ccgataccgg atctgtaaca cgtacgtaat acctacccca
     1501 ttcggctgac ttaaattgca cattagcttt accctgtgca tttgttttta cagaatagct
     1561 ctgtatagga ttttccattt cagacttata atagctgtac gagttagaat tatcttcatc
     1621 ccaccaccaa gaccagtcaa ccctgaaaat ttcaactttg agggctgtct ggctgatacc
     1681 attaccatct gtactaatac tggcaagaga aatcggcaca ttttcgttta cgttatatgt
     1741 aggagcacca tagctgtttt tcaatacaga cacgccacaa tatctattga acggatggta
     1801 agtgacattg acattatcaa catttggttc gccaccttgt tcgaaaactt ttgttttaaa
     1861 attgactacc attggcccag ttgcgttcga ttctttgggc agaagttttt gtgaaatttt
     1921 cgtattccca agatcattta atctgccctt aaaccactcg aatgtagtat aaaatggagc
     1981 ctcgcggata ttattaatcc ggaaatcctg aaatttttta aacccttctt tagactgcca
     2041 catagacaca tcaatacggg catctaagtt tttagcggga gcaccataca accagttggc
     2101 agaaagtatg cagttgacag gttcgtcgct tgatgtcagt tcttttttgc cgaaatcaag
     2161 ctttattcgc attctattag gcttgatatt ttcaattttg acaggaaaat taaatactgc
     2221 tccacctact ttaagaactg ctttccagtt gcccgtaact gcatcgggcc tggtggcaat
     2281 gtatatcgga taaagtctgt ctgaggatga agatagaacc tgcgaacttt gaagtattcc
     2341 acgcgaatca aaaaattcga aattgaccgg cacaccgtcc ggaatattag tctgatcacc
     2401 tgactgcatc ataaaattga ggtaaagtga atcaccgggt cgccacactc ccctctctcc
     2461 atacaaaaag ccctttacac ccttctgtac gtcttcacct accacatcga actgacttat
     2521 attaagtgct tcgttatcca tcagtcggag gtagcccttt ctgccacctt gctgagcgac
     2581 aactgcaaat ggttttacct tgaatcctgt agccatagca ataccatcag cattggtttt
     2641 ggccgtcttt acctgctgta gttgatagtc atagaatgtt atatccactc catttaatgg
     2701 ttttgttgta ttgaggtcag tagcgataac cattacttca ccagctttac ccgatttggc
     2761 aatcagaccg atgttgcttg aatataccat aacattcaga aaccgacttg agttgtagta
     2821 c
//
LOCUS       DAOQQY010000331         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18888_length_2811_cov_5.120827, whole genome shotgun sequence.
ACCESSION   DAOQQY010000331 DAOQQY010000000
VERSION     DAOQQY010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18888_length_2811_cov_5.120827"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="PLE23_11955"
     CDS             complement(<1..1642)
                     /locus_tag="PLE23_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMX84023.1"
                     /translation="MMFKKSKRRDLVVLSRVIALASPYKSLFLIASALAIILAPLSTL
                     KPKLIQEMTDNYIVNKDLDGMKMLFFILLGVLVIEALFNYMFNFITSLLGANVVKDLR
                     LRVYKTVTSLRLKYFDITPIGNSTTRTINDIETINSIFSEGIITIIADILTLIAVLGM
                     MFYTSWKLTLFCMITLPFLMVAAYIFKEKVRISFQKVRNEISKMNSFLQERISGMKIT
                     QIFNAEEEDRAKFRQINRDYTQANLDSILYYAVFFPVVEIISAASLGLMVWYGARGML
                     HDSISIGVMVSFPIYISMLFRPIRMLADKFNTLQMGIVAADRVFLLIDETELKEQGGT
                     LKPDKLEGKVSFDRVSFAYNEGDYVLKDVSFEVQPGHSLAIVGSTGSGKTTIISLINK
                     FYEIQKGHIFIDDKDINEYDLQALRRRIAVVLQDVFLFTGSVLENISMRDPNITREQC
                     IEAAKWIGAHEFIVNLPGGYNFEVNERGTTLSMGQRQLISFARALVFNPDILILDEAT
                     SNIDTESEMVVQHAIEKLIEKRTSIIIAHRLSTIKKADN"
     gene            1878..>2811
                     /locus_tag="PLE23_11960"
     CDS             1878..>2811
                     /locus_tag="PLE23_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMX84024.1"
                     /translation="MYDQRPWLKLYPAGVPSNINPDSYPLLIDLFTETFEKYRHKKAF
                     NCMGAELTYDQVDTYSQNFAAYLHSRGLEPGDKIAIMMPNLLQYPIVLFGALRAGLVI
                     VNTNPLYTAREMKHQFIDSGAKAIVIAENFASHLQQILPETQIKTIITTTIGEMLGFF
                     KGKIVDFVVSKIKKMVPAFELPNTVTFKYALSEGAKFKVQAFNSHPDDVIMLQYTGGT
                     TGVAKGAMLTNRNLVANMVQIKSWLMPSGIGEGDIGLSPLPLYHIFAFSVNCLALFSV
                     GVVTVLVVNARDIPSVMKEFKQHKVTIMTGVNTLF"
BASE COUNT          791 a          618 c          564 g          838 t
ORIGIN      
        1 tattgtctgc ttttttgatg gttgaaagcc tgtgtgcaat gataatagaa gtgcgctttt
       61 ctatcagttt ttcgatagca tgctgtacta ccatctccga ttcggtgtca atgttggagg
      121 tagcttcgtc aagtatcagt atatccggat tgaataccag ggcgcgggca aatgatatga
      181 gctgccgctg acccatcgac aaggtggtac cacgttcgtt tacttcaaag ttatagcctc
      241 ccggcaagtt gacgataaat tcatgtgcgc caatccactt tgcagcctct atacattgct
      301 cgcgtgtgat gttggggtcg cgcatactga tgttttcgag tactgagcct gtaaacagga
      361 atacatcctg caatacaacc gctattcgtc gtctgagagc ctgcagatcg tattcgttga
      421 tgtctttatc gtcaataaaa atgtgtcctt tctgtatttc gtaaaatttg ttgataaggc
      481 tgatgatggt tgtcttgccc gatccggtac tgcctacaat ggccagcgag tgtccgggct
      541 gtacttcaaa acttacatct ttcaagacat agtcgccctc attgtacgca aagctgaccc
      601 ggtcgaagct aactttgcct tccaacttat ccggcttgag tgttccgccc tgttccttta
      661 actcggtttc atctatgagc aaaaatacac ggtcggcagc tacaataccc atctgtagtg
      721 tgttgaactt gtctgccagc atcctgattg gtctgaataa catcgatata taaataggaa
      781 atgaaaccat cacacctata gaaatcgagt catgaagcat gcctctggca ccataccata
      841 ccatcagccc cagtgatgct gctgaaatga tttcaactac cggaaaaaat acggcatagt
      901 ataaaatgga atcaaggttt gcctgtgtgt agtcacggtt tatttgtctg aattttgccc
      961 tgtcctcctc ctctgcattg aatatctggg tgattttcat tccgcttata cgctcctgaa
     1021 gaaacgaatt catcttcgaa atctcgttgc gtaccttctg gaacgaaatg cggactttct
     1081 ccttaaatat ataagctgct accattagaa aaggtagggt aatcatacaa aatagcgtga
     1141 gtttccaact ggtatagaac atcatcccca atacggcaat cagtgttagt atgtcagcaa
     1201 tgatcgttat gattccttcg gaaaatatag agttgatggt ttcgatatca tttatagtgc
     1261 gtgttgtaga attgcctata ggagtaatgt cgaaatactt caacctgagc gatgtaacag
     1321 ttttataaac ccgcagccga aggtctttta ctacatttgc ccccagcaga cttgtgataa
     1381 aattaaacat gtaattgaac aaagcttcta tcaccagaac acctaacaaa ataaagaaca
     1441 acattttcat gccatccaga tccttgttga cgatgtagtt gtccgtcatt tcctgaatga
     1501 gctttggctt cagagttgac aaaggagcca gaataatcgc aagtgctgaa gcaatcagaa
     1561 ataaactctt gtacggactc gcaagggcaa tcacccttga cagtacaacc agatcgcgcc
     1621 gtttcgattt cttgaacatc atgcaaaaag acaaaattaa tcggattttg ttctactttt
     1681 atttattaaa caatagtatt atagtatttt attaaagcat tttatgaatt aacgtgaaat
     1741 tttaccccga gaaaaggtat ttattacgct cggttttgta acatttaata tttccggtcg
     1801 atttttttcg gtttttttaa tcaatagcat taactttacc ttgaatgctt taacatcatt
     1861 ttattaattc tatagttatg tacgatcaaa gaccctggtt aaaattatat cctgccggag
     1921 tgccatccaa tatcaatccg gattcttatc ctttgctgat agaccttttt actgaaacct
     1981 tcgaaaaata cagacataag aaggctttca actgtatggg ggcagaactt acatacgacc
     2041 aggttgatac atattctcaa aattttgctg cttaccttca ttccagaggt cttgaaccgg
     2101 gcgataagat agccattatg atgcccaatc tgctccagta cccaatcgta ttgtttggtg
     2161 cactcagggc aggcctcgta attgtcaaca ccaacccact ctatactgcc cgggagatga
     2221 agcatcagtt catagacagt ggcgccaagg ccatcgttat agccgaaaat tttgcctcgc
     2281 accttcagca gatattgccc gaaacacaaa tcaaaacaat catcaccact actattggcg
     2341 aaatgctggg tttctttaag ggtaagatag tagattttgt ggtttcgaaa atcaagaaaa
     2401 tggtgcccgc cttcgagctg cccaatactg ttacatttaa atacgccctg agcgaaggag
     2461 caaaattcaa ggtacaagcc ttcaattccc atcccgatga tgtaatcatg cttcaatata
     2521 ccggaggcac caccggagta gccaaaggtg ccatgctcac caaccgcaac ttggtggcca
     2581 atatggtgca aatcaaatca tggctcatgc cttctggcat cggggagggc gatattggct
     2641 tatcaccatt gccattgtac catattttcg ctttctcagt caactgcctt gctttgttca
     2701 gtgtaggcgt agtaacggtt ttagtggtaa atgcccgcga tattccatcg gtgatgaagg
     2761 agttcaagca gcataaagtg accatcatga caggggtcaa cacgttgttc a
//
LOCUS       DAOQQY010000332         2811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_18891_length_2811_cov_4.556241, whole genome shotgun sequence.
ACCESSION   DAOQQY010000332 DAOQQY010000000
VERSION     DAOQQY010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18891_length_2811_cov_4.556241"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(120..1667)
                     /locus_tag="PLE23_11965"
     CDS             complement(120..1667)
                     /locus_tag="PLE23_11965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84025.1"
                     /translation="MKKILFILLSIWAAALSAQHTKSLVYSNPLIGGNEGVWQIVSFG
                     EGFLVVAPGICLPAVKGCTSIRNYSYENQVLWETFWDTIAPPHFKVIATSDSAIYMAS
                     WYRSPGVDEEYINKLDLQGNVLKTVQVDYEANPKGENKAIVNVSLIGDTVVICHNKWL
                     YPGPDSLFLKYYDKDLNLVKELKLTENLKDDVAYGIHVTPDSNYLISKLTGYLPYYLH
                     IMKTDRQGNVLWVHEDTLNRTLEPFQDFMVTNDKDSSYTFFSFKEESIKTPVSQFPWV
                     RDYSVIFKRSAIDNSLIWYKPLLSFHYSLIYFLSGLPRRNDGSMIGGGMADRNLAKDK
                     KPVDLVGWLFKIDPAGELKWSRFYKDDRYEYFGSYFVTATELSNGDLVAGGDLDQLSI
                     DPPGLSWVLHLDSMGCLTPGCVDTMLYTRTEEVNGKLLVKEVFFKSFPNPVHDRLSVE
                     FYNQSHRPASLLRVLSIDGKEYYRTELHNGDKSLTVNMSGYPPGMYLVQYSSGSLLLQ
                     TEKVVKE"
     gene            complement(1681..>2811)
                     /locus_tag="PLE23_11970"
     CDS             complement(1681..>2811)
                     /locus_tag="PLE23_11970"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84026.1"
                     /translation="GYDYDFELKSGNIFPYQGVPGNPAENCFSEIKAISPGVYTTNML
                     VPNKSSFFTYYVDKDIPDTTHCEYPKRKGKNNYKIELTPNNKSGCDLDYGGYVNELPP
                     PPYDLTSFIQYKNAVNNAYQALVADPQNTVLDYQYHNLSYKKQGIFEAILADAIEAND
                     TSLVHQILNEEGSLSSMHLRYGYYVRQNDYNYALRILNSLPDSTEDDYDYKYIQHVNL
                     DRLMESESPYIPSAGVVDSLQQIINKNSLNSGMADAIGYVLGLELDNTATYPLAICDN
                     GNNKNPVVPREINSAHTDAVNIYPNPVNDLLVIELGDNRERYDQVSILSVMASVISAS
                     ELKAGTVKHEINTARLANGIYIVVLSSSTTGTRTPGKIIVNH"
BASE COUNT          695 a          661 c          605 g          850 t
ORIGIN      
        1 ataaaacccc gaccgatcca ccgcaaagga gcaaaaaggc gtccgttttt ctgaaccctt
       61 gtaggaaaaa cagccttttc gctccggttc ggcttctatt gctaacattg catatctgcc
      121 tactccttca ctaccttctc ggtttgcagt agcagactgc ccgatgaata ttgaaccaga
      181 tacatgccgg gtggataacc gctcatgttg acagtaagac tcttgtctcc gttatgcagc
      241 tctgtacggt aatactcctt gccgtctatc gacaataccc tgagcaacga tgccgggcgg
      301 tgcgactgat tgtagaattc tacgctcagc cggtcatgca ccggattcgg gaatgatttg
      361 aagaacacct ctttcaccaa cagcttgccg ttcacttctt ctgtgcgggt gtacagcatc
      421 gtgtccacgc agccgggtgt cagacagccc atggagtcaa ggtgcagcac ccaactcaaa
      481 cccggcggat caatgcttag ctgatccaga tcgccgcccg ctacgagatc tccgttgctg
      541 agctccgtgg ctgtgacgaa ataactacca aaatattcgt agcggtcgtc tttataaaac
      601 cggctccact tcaactcccc ggcagggtct atcttgaaca accagcccac caaatctaca
      661 ggttttttat ctttagccag attcctatcg gccatgccac ctcctatcat gctaccgtca
      721 ttccttcttg gcaagccgct taaaaaatat atcagactat aatgaaatga taataaaggt
      781 ttataccata tcagtgaatt gtcgattgcc gaacgcttga agattaccga atagtctctg
      841 acccatggaa actgtgaaac aggtgttttg atagattctt ccttaaaact gaagaaagta
      901 taactgctat ctttatcgtt tgttaccata aaatcctgaa aaggttctaa cgttctattt
      961 aaagtatctt catgcaccca cagcacattc ccctgccggt cggttttcat gatatgaaga
     1021 taatacggta aatagcctgt cagtttggag atcaggtaat tgctgtcggg cgttacatgg
     1081 ataccgtagg caacatcatc cttcaagttt tccgtcagct tgagctcctt taccaagttc
     1141 aggtctttgt cgtaatactt cagaaacagc gaatcgggcc ccggatacag ccatttgttg
     1201 tggcagatta ccaccgtatc gcctatcagg ctcacattga cgatggcctt gttttcgcct
     1261 ttggggtttg cctcatagtc cacttgtacg gtttttagca cattgccctg caggtcgagt
     1321 ttattgatat actcttcatc cacaccgggg ctccgatacc aactggccat atatatggcc
     1381 gaatcggatg ttgctattac tttgaaatga ggcggtgcaa tcgtatccca aaaggtctcc
     1441 cacagaacct ggttttcgta ggagtagttg cgaatcgagg tacagccttt tactgccgga
     1501 agacagatgc ccggtgcaac cactaaaaat ccctcaccaa aagatacaat ctgccaaaca
     1561 ccctcattgc ccccgatcaa aggattgcta tataccaggc tcttcgtatg ttgtgccgat
     1621 aaggccgccg cccagatgct gagcaggata aatagtattt ttttcatctc tcaacagttt
     1681 ttaatggtta acgatgatct tacccggtgt acgtgtgccg gtggtagatg atgaaagcac
     1741 cacgatatat atcccattgg ccagtcgggc tgtatttatc tcatgcttta cggtcccggc
     1801 ttttagttcg cttgcactta tcaccgatgc cattaccgac aatatgctta cttggtcata
     1861 tcgctcgcgg ttgtctccaa gttctatgac tagtaggtca ttaacagggt taggatagat
     1921 gttaactgca tcagtatgtg cactgttgat ttcacgtggc actactggat ttttgttgtt
     1981 gccattatca catattgcca gtggataggt tgccgtatta tccaattcaa gacccagcac
     2041 gtagccgatg gcgtctgcca taccgctgtt caggctgttc ttgttgatta tctgctgtaa
     2101 gctatctact acaccagccg atgggatgta aggtgattca ctttccatca acctgtccag
     2161 gttaacgtgc tgaatatatt tatagtcata gtcgtcttca gtagaatcgg gtagagaatt
     2221 tagtatcctg agtgcatagt tatagtcgtt ttgcctcacg tagtatccgt accggaggtg
     2281 catgcttgat aatgagcctt cttcattcaa tatctggtgt acaaggctcg tgtcgttggc
     2341 ttctatggca tccgcaagta ttgcttcgaa gatgccctgt tttttgtaac taagattgtg
     2401 gtactgatag tcaagtactg tattttgcgg gtcagccact aatgcctgat aagcgttgtt
     2461 aaccgcattt ttgtattgta taaagctggt aaggtcatac ggcggaggtg gtagttcatt
     2521 gacgtatcct ccatagtcca agtcgcagcc tgatttatta ttgggagtta attctatttt
     2581 gtaattgttc tttccttttc gttttggata ttcgcaatgt gtggtgtccg gaatatcttt
     2641 atcgacataa tatgtgaaaa atgagctttt attcggtacc agcatgtttg tggtatatac
     2701 gccaggactt attgctttta tttcagaaaa acaattctcg gcagggttgc ccggaacgcc
     2761 ttgatatgga aatatatttc cggatttaag ttcaaaatca taatcatatc c
//
LOCUS       DAOQQY010000333         2792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_19115_length_2792_cov_6.235294, whole genome shotgun sequence.
ACCESSION   DAOQQY010000333 DAOQQY010000000
VERSION     DAOQQY010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19115_length_2792_cov_6.235294"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLE23_11975"
     CDS             complement(<1..441)
                     /locus_tag="PLE23_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HMX84027.1"
                     /translation="MTEPNVADLQTPSRPRKPEWLKVKLPVGESYRNVRNIVNENKLH
                     TICQSGNCPNMGECWGMGTATFMILGNVCTRSCSFCAVKTGRPTEYDEDEPRRVAEAI
                     RLMQVKHAVITSVNRDELKDKGADIWHKTVQLVKEFNPLTTIETL"
     gene            complement(443..1231)
                     /locus_tag="PLE23_11980"
     CDS             complement(443..1231)
                     /locus_tag="PLE23_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84028.1"
                     /translation="MAYIRLNIILLILLASSFVGAQSFSGYDTGVESKGILYNKEKAF
                     KARLQTNGFNIGWYSGKIRTYYKTNFTHYEIGYLKNNHELSQRFDYSNPFTSQSARSF
                     VFGKQNSLYVARVGFGQKYYYSDKQRHRGISIGSTYEFGGILGLKKPYYLELIRYKDN
                     GSSAYLSKEKYSEENKEVFTDLSRIFGGPSFFEHWDEVRPVPGVYGRAGMLFEWDTLE
                     GYFTGVEAGIQADAFPTTVNLMIDQPKQIIFINLYLSVEIGKRK"
     gene            1332..2630
                     /locus_tag="PLE23_11985"
     CDS             1332..2630
                     /locus_tag="PLE23_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMX84029.1"
                     /translation="MIIIYTKRCSARLLYTARHIFEHVLGVEYRVSENLNGKNDGDIV
                     LTYGFTASAGELSMYASDILFEDTGPHIDTYHKIIEKPWPYFKAPFGSFDFCEDVFAL
                     CFFSISRYEEYVLPIKDKYGRFRACDSVFSRDKVLQTPHVDTMIFEFARKIQSVFHQF
                     QFSNKKTKKLLTIDVDHAWKYRNKGFIRHLAAFGKNVLQLRLDLVAERMKVLTGILPD
                     PYFSFDQLDKLSTLVEMRYFILLGDYNKIDINIHHKSMEFRQLLSSLSDRYPVGIHPS
                     SKSSNNTLQLIKERKRLEEITKKPIYDSRQHFLLLTMPGTYHALLEAGITDDYTMGFA
                     DNIGFRAGTAFPFYWFDLSTDTETLLLVHPFVAMDVTIRDYLNLKPSEAFGKMKSMLD
                     GVKQNGGTFTLLWHNSSFTDAEGWSEWKSGLVEFFEGNQE"
BASE COUNT          809 a          541 c          572 g          870 t
ORIGIN      
        1 aagtgtttca atggtagtaa gcggattgaa ttcttttacc agttgcacag ttttgtgcca
       61 aatgtcggct cctttgtctt tgagttcgtc gcggttaaca gatgtgatga ctgcatgctt
      121 tacctgcatc agccggatgg cttctgctac gcgtctaggt tcgtcttcat catactcggt
      181 ggggcggccg gtttttacgg cgcaaaaaga gcaagaacgt gtacaaacat tgcccaatat
      241 cataaatgta gcggtaccca tgccccaaca ttctcccata ttcgggcaat tgccactctg
      301 gcagatggtg tgcagcttgt tttcgttaac tatattccgg acatttctgt atgattcgcc
      361 tacagggagc tttactttta accattccgg ttttctgggt cttgaaggtg tttgcaggtc
      421 agcaacattg ggttcggtca tattattttc tctttcctat ttctacgcta agatacaaat
      481 ttatgaaaat tatttgttta ggctgatcaa tcatcaggtt aacagtagta gggaaggcat
      541 ccgcctgtat tcctgcttcc acgcctgtaa aatatccttc aagtgtatcc cattcaaaca
      601 acataccggc cctcccatag acacccggca caggtcgtac ttcatcccaa tgttcgaaaa
      661 aactcggccc accaaaaata cggctaaggt cagtaaatac ttccttgttc tcttcgctgt
      721 atttttcttt tgataaatag gcactcgaac cattgtcttt gtagcgtata agctccaagt
      781 aatatggttt tttaagaccg agtatgcctc caaactcata ggtagagccg atggaaatac
      841 cacggtgcct ttgtttgtct gaataataat atttctgccc aaaacctact cttgctacat
      901 agagcgaatt ttgcttgcca aacacaaacg aacgtgccga ctgacttgtg aacggattgc
      961 tgtaatcgaa tcgctgactc agttcgtgat tgtttttaag ataccctatt tcataatggg
     1021 taaaattggt cttgtagtat gtgcggattt tgcccgaata ccagccaatg ttaaatccgt
     1081 tggtctgtaa ccgcgcttta aaagcttttt ccttattata tagaattcct ttagactcta
     1141 cacctgtatc ataaccacta aatgactgag caccaacaaa cgaagatgcc agcagaatta
     1201 ataggataat atttagccgg atatacgcca aagtttataa tttcgtttag aattaaaaaa
     1261 ttaatttaaa gatataaaat tattttggct tttcaaaaaa tgtagttttt ttttgaaaaa
     1321 agttttatcg aatgatcatc atctacacaa aacgctgttc tgcacgcttg ttgtacacag
     1381 caagacatat ttttgaacac gtgcttggtg tagagtatcg tgtgagcgaa aatctgaatg
     1441 ggaagaatga cggagatatt gtgctgacct atggttttac agcatccgca ggcgagttga
     1501 gtatgtatgc ttccgacatt ctttttgaag atacagggcc tcatattgat acctaccata
     1561 agattattga aaaaccatgg ccgtatttca aggcgccttt tggtagcttt gatttttgtg
     1621 aggatgtttt tgcgctgtgt tttttttcca ttagccgata cgaagaatat gttctgccga
     1681 taaaggataa atatggtcgt ttcagagctt gtgacagtgt tttttcaaga gataaagtgc
     1741 tccagacacc acatgtcgat acaatgatat ttgaatttgc cagaaagata caatcagttt
     1801 ttcatcaatt tcaattttca aataaaaaga caaaaaaact cctgaccatt gatgtagatc
     1861 atgcctggaa gtacagaaat aaaggtttta tccgccacct ggcagcgttt ggtaaaaatg
     1921 tactccaact tagattagat ctggtggccg aaaggatgaa ggtactgacc ggaatattac
     1981 ctgatccata ttttagcttt gatcaattgg acaaattatc gactttggtc gaaatgcgtt
     2041 attttatcct gcttggtgat tacaacaaaa ttgacattaa tattcaccac aaatcaatgg
     2101 agttcaggca attacttagt tcattatctg accggtatcc ggtaggaatc cacccttcat
     2161 caaaatcatc gaacaataca ttgcagctta taaaagagcg gaaaaggctt gaagagatta
     2221 caaaaaaacc gatctacgat tcgagacagc attttttatt gttgacaatg ccgggtacgt
     2281 atcatgcatt acttgaggcg ggcatcacag atgactatac aatgggcttt gcagacaata
     2341 ttggttttcg ggcaggtacg gcctttccgt tttattggtt tgacctaagc acggatacag
     2401 aaaccctgtt gttggttcat ccttttgtgg caatggatgt taccatacgc gattatttga
     2461 atcttaaacc ttcagaagca tttggtaaaa tgaaatcgat gttggacgga gtaaaacaaa
     2521 atggcggcac attcacattg ctctggcaca acagctcatt tacggatgcc gaagggtggt
     2581 ctgaatggaa aagcggatta gttgaatttt ttgagggcaa tcaggaatga tatttgccgg
     2641 aatttcagta tatttgaaaa aactattttt actatgataa atgtgcttgc acaaacgaat
     2701 tctgtggcca ataattttct ggctgaaatc agggatatcg aagtacaaag agatgcgctc
     2761 cgcttcagaa gaaacatgga gcggctcggt ga
//
LOCUS       DAOQQY010000334         2779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_19288_length_2779_cov_6.963289, whole genome shotgun sequence.
ACCESSION   DAOQQY010000334 DAOQQY010000000
VERSION     DAOQQY010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19288_length_2779_cov_6.963289"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLE23_11990"
     CDS             complement(<1..540)
                     /locus_tag="PLE23_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMX84030.1"
                     /translation="MNIIIPMAGRGTRLRPHTLTIPKPLIKIAGKPIVQRIVEDLASS
                     YRGTIDEVAFVIGDFGREVEEELLKVAESVGAKGKIFYQQKALGTAHAVYCAAESLTG
                     NCMIAFSDTLFKADFSFDENEDGYIWCMRVPDPKAYGVVNLNDAGYITEFVEKPETFV
                     SDLAVVGIYYFKDGDGLKAE"
     gene            complement(773..2449)
                     /gene="ettA"
                     /locus_tag="PLE23_11995"
     CDS             complement(773..2449)
                     /gene="ettA"
                     /locus_tag="PLE23_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HMX84031.1"
                     /translation="MSDHKIIFSMSGVSKTYPPQKQVLKNIWLSFFYGAKIGVLGLNG
                     SGKSSLLKIIAGLDQNYEGKIVFDKTFKIGYLAQEPELDNSKTVRQIVEEAVQPVVDM
                     LKRYEAINMRLAEPISDNEMMKLIEEQGEVGEQLETMNAWDLDHRLETAMDALRCPEG
                     DVEVNVLSGGERRRVALCRLLLSDPDILLLDEPTNHLDAESVEWLEQYLKNFPGTVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKARRLEQEEKTESKRRKTLERE
                     LEWVRMSPKARQSKGKARLNAYDKLANEELKDKEQKLELYIPPGPRLGDKVIEFDKLT
                     KGFGNRILIDNLSCSIPRNAIVGIIGPNGMGKTTLFRMIMGSEKPESGSIIIGDTVKP
                     SYVDQSHADLDPEKSVYEVISSGNDLLNVNGTQVNARAYISKFNFSGTDQQKKVGVLS
                     GGERNRVHLAMTLKDGGNVLLLDEPTNDIDVNTLRALEDALEDFAGCVMVISHDRWFI
                     DRLATHILAFEDNGEVVFFEGNFTEYEQAKKARLGDITPHRPRFKHLINN"
     gene            complement(2583..>2779)
                     /locus_tag="PLE23_12000"
     CDS             complement(2583..>2779)
                     /locus_tag="PLE23_12000"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMX84032.1"
                     /translation="FIINEDGAIQFVSVKKSDNPALNEAATNVIQNMNKYFSEDPWVP
                     GFHQGNRVATKMLVPVQFKL"
BASE COUNT          666 a          631 c          615 g          867 t
ORIGIN      
        1 ttctgcttta agaccatcac cgtccttgaa ataataaata cctactacag ccaggtccga
       61 cacgaaagtc tccggttttt ctacaaactc ggttatgtag ccggcatcat tcaggttgac
      121 cacaccgtag gccttagggt ctggcacacg catacaccag atgtagccgt cttcgttttc
      181 atcaaaactg aaatcggcct tgaagagtgt atcagaaaag gctatcatac agttgccggt
      241 gagtgattct gctgcacaat agacagcatg tgccgtaccg agtgcctttt gctggtaaaa
      301 tatttttcct ttagcgccga cagattctgc aacctttagt aactcttctt ctacttcacg
      361 tccaaaatca cctataacaa atgctacttc atcaatggtg ccacgatatg atgatgccag
      421 gtcttcgaca atacgttgta cgatgggttt gccggctatt tttattaatg gttttgggat
      481 ggtaagtgta tgtggtctca gtcgggtacc acgccctgcc attggtatga tgatattcat
      541 ttaatctgat ttttttaacg gtttacaaag atacaaagtt aaggcgattt catcgtccaa
      601 cgaatatttt acttacacaa aagaccaaaa agtaattcag gccttctatg gcgaactata
      661 tattcgtaaa gtggccataa tagggcatca cattttacac acaaatcaaa agcttgattc
      721 aagatgtgca aatattattc gtccggcagt aattttataa aaaagtccac aatcaattgt
      781 tgatcaggtg tttgaagcgt ggtctgtgcg gtgtaatatc gccaagtctt gcttttttcg
      841 cttgttcgta ttccgtgaag tttccttcaa aaaatactac ctcaccattg tcttcgaatg
      901 ccagtatatg tgtagccagc ctgtctataa accatcggtc gtgactgatg accatgacac
      961 aaccggcaaa atcctccagg gcgtcttcca gtgcacgtag tgtgttgaca tcgatgtcgt
     1021 tggtaggctc atcgagtagg agtacgttgc caccatcttt aagtgtcatg gcgagatgta
     1081 cacggttacg ctctccaccc gagagtacac ctactttttt ctgctggtcg gtacccgaga
     1141 aattgaattt gcttatataa gcccgagcat tcacctgtgt gccgttgaca ttcagcaggt
     1201 cgttaccact gcttattact tcatacactg atttctcagg atcgaggtcg gcgtgcgact
     1261 gatctacata gcttggtttt acggtatcgc ctatgataat gctacctgat tcaggttttt
     1321 cgctacccat aatcatcctg aatagcgtgg tttttcccat gccgttaggg ccaatgatac
     1381 ccacgatggc gttgcgtggt atgctacagc tcaagttgtc aatcaaaatc cggttgccga
     1441 atcctttggt tagtttgtca aactcgataa ccttgtcgcc aaggcgtggt ccgggtggaa
     1501 tgtacagttc gagtttttgt tccttatctt ttagttcttc attggcgagt ttgtcataag
     1561 cattcagacg agccttacct tttgactggc gagctttggg cgacatacgc acccattcga
     1621 gttcgcgttc gagtgttttt ctacgtttgc tttccgtctt ttcttcctgt tcaaggcgtc
     1681 gtgccttttg ttcgagccac gagctgtagt tgccctccca tggtatgccc tcacctcggt
     1741 cgagttcgag gatccagcct gctacgttgt ctaggaaata acggtcgtgc gtgacagcga
     1801 taaccgtacc cgggaaattc ttaaggtatt gttccagcca ttcgacgctt tcggcatcga
     1861 ggtggttggt aggctcatcg agcaatagga tatcagggtc gctcagtagc agacgacaca
     1921 gagccactct ccttctttcg cctcctgaca acacattaac ttctacgtca ccttccgggc
     1981 agcgtagagc atccatggcg gtctcgagcc ggtggtcgag gtcccacgca ttcatggttt
     2041 cgagttgttc gcctacttcg ccctgttctt cgatgagttt catcatttcg ttatcagaga
     2101 tgggttcggc aagacgcata tttattgctt catatcgttt gagcatatcc accacaggtt
     2161 gtacggcttc ttctactatt tggcggactg tcttggagtt gtcaagttcg ggctcttgag
     2221 cgaggtagcc gattttgaat gttttgtcaa atactatttt gccttcgtaa ttctggtcaa
     2281 gacctgcaat gatttttagg agcgaagatt ttccgctgcc gttgaggccg agaacaccga
     2341 tttttgcacc gtagaagaac gaaagccaga tatttttaag tacttgcttc tgtggtgggt
     2401 aagtcttgct tacaccagac atcgaaaaga tgattttatg gtctgacata ttgtatttct
     2461 ttgattgttt gaggtgcaaa tatggcacga aatccgcttg ttttcaaaag ttaagcggca
     2521 aatttattaa ttcattgccc ggtcatgggc ctatatcatt caggtatatc aatgaaaatg
     2581 tatcaaagtt taaactgtac aggcaccaac attttagtcg ccacgcgatt tccctgatgg
     2641 aatccgggca cccagggatc ttcagagaag tatttgttca tattctggat gacattcgta
     2701 gcggcttcat tcaatgccgg attgtcagat ttttttaccg atacaaattg gatggcgcca
     2761 tcttcattga tgataaact
//
LOCUS       DAOQQY010000335         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_19517_length_2756_cov_6.136616, whole genome shotgun sequence.
ACCESSION   DAOQQY010000335 DAOQQY010000000
VERSION     DAOQQY010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19517_length_2756_cov_6.136616"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLE23_12005"
     CDS             <1..431
                     /locus_tag="PLE23_12005"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84033.1"
                     /translation="GEKADLWDIANTCYDDGGKIVDQARALWLQLYDQYMMQAECVEG
                     RQADEEQSSFNKAESGSVYLYPNPASGILSIVLNNVPEQSTELQIFDLRGREMPQNTI
                     RSLGDDVITLDIKDYLEGMYIVRLKSANQTISHKLIVVRR"
     gene            505..2139
                     /locus_tag="PLE23_12010"
     CDS             505..2139
                     /locus_tag="PLE23_12010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84034.1"
                     /translation="MKYILATLSLIMIYNTCMGQTTTRIYKNEYFDNSSGNGNDVYGL
                     YSGKGVFLDNGDFVIGNQWNPVKGSSTLVPTITCLGEDGEINWSYRMTEMELALDGLS
                     VTNNNEIIIPTSLSKVNSLINEYAYIFKLDGNGNMKWSKGIKGYNFVKTIVTGDGNIV
                     ALCRELYQQGSTLIKFTPAGKLLWAYSFEQFYVNEISETGDELLMTGSFYDKENGINY
                     WGIVWLDQGGTKTDSRKFFMGTGKSLALKSVKVDKDGYTVAGGVLSIGGTPYIQDYGL
                     LVKFNKDRSILWLKKYESGPRKIGFENMINRNGNWLLNGWFYKDDKVASGFVVESDKD
                     GNISRASGLKIDTAGIALLRSGVNPSGQIYYSGILVRTLPPKEFAYTMDDLIVFQTEA
                     GWIECFEHTLPMTVSDFTAYTYTDVDIMQDTVTTLKFLDLKVGIEPDDYITGYTICET
                     TATTEVSAEAGALRLYPNPVSTTLSIDVEQAGYGTVREQTVDITDLLGRKLYEVEVGY
                     DGHAEVDTKTLPPGMYIAVLRADMHQVQAVKFVVGR"
     gene            2343..>2756
                     /locus_tag="PLE23_12015"
     CDS             2343..>2756
                     /locus_tag="PLE23_12015"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX84035.1"
                     /translation="MVKREAEQELRDLAQAFKAVAVTGPRQSGKTTLVKYVFPGKPYV
                     NLENPDIRLFATEDPRGFLSNYPQGAVLDEVQRVPHLFSFLQEILDNATTNGMFVLTG
                     SNNFLLQENISQSLAGRVAYLTLLPLSISEMKISGN"
BASE COUNT          848 a          462 c          678 g          768 t
ORIGIN      
        1 ctggcgaaaa agctgatttg tgggatattg ccaatacttg ttatgatgat ggaggcaaga
       61 tagtggacca agccagagca ctgtggttgc agttgtatga ccagtatatg atgcaggcag
      121 aatgtgttga gggtagacaa gctgatgagg aacaatccag ttttaacaag gctgagtcag
      181 gctcggttta tttgtatcct aacccggctt ctggaatact cagtattgtg ctgaataatg
      241 tgcctgagca aagcacggaa ttacagattt ttgatctcag aggccgcgag atgccacaaa
      301 atactatccg aagtttggga gatgatgtca taacgctgga tattaaagat tatcttgaag
      361 gtatgtacat agtgcggcta aagagtgcaa accagaccat cagccataag cttatagtag
      421 ttcgcagata aattcttttg taaaaaggct gagctattgg tttatcagct cagcctgatt
      481 ttctaattaa aaaaattgaa tgatatgaaa tatatactag cgacattatc attgataatg
      541 atatacaata cctgtatggg gcaaactacc acaaggattt ataaaaatga atattttgac
      601 aattcatctg gtaatggcaa tgatgtttat ggtttatatt ccggtaaagg agtttttctt
      661 gataacggtg actttgtcat tggtaaccaa tggaatcctg taaagggtag ttctacactt
      721 gtacctacaa ttacctgttt gggtgaagac ggagaaataa attggtctta cagaatgact
      781 gaaatggagc ttgctttaga cggcttaagc gtaacgaata acaatgagat tatcatccca
      841 accagcttaa gtaaggttaa tagcctaatc aatgaatacg cctatatttt taaattagac
      901 ggcaatggaa atatgaagtg gtcaaagggc ataaaagggt ataattttgt caaaacaatt
      961 gtaacaggcg acggcaatat tgtagccctt tgtcgagagc tatatcagca aggtagcacg
     1021 ctgattaaat ttacacctgc cggtaagctg ctctgggcat atagctttga acagttttat
     1081 gttaatgaga tatcggagac aggagatgag ctgttgatga caggctcttt ttacgataaa
     1141 gagaatggta ttaattattg gggtatagtg tggttagacc agggcggcac gaaaacagac
     1201 agcaggaaat tttttatggg aaccggcaaa tctcttgcat tgaagtctgt aaaagtggac
     1261 aaagatggct atacggttgc cggaggggtg ctgtctatag ggggaacgcc ctatatccag
     1321 gattatggac ttttggtgaa attcaataaa gaccgcagta ttctatggct gaagaaatat
     1381 gagagcggac cgcgaaagat agggtttgag aacatgataa atcgcaatgg gaattggtta
     1441 ttaaatgggt ggttttataa ggatgacaag gtggcatccg gctttgttgt agaatcagat
     1501 aaggacggga atataagccg ggcttctggg ctgaagatag acacagctgg aattgcactg
     1561 ttacgaagtg gagtgaaccc tagtggacag atttactatt cgggtatatt ggtacgtaca
     1621 ttgcctccta aagaatttgc atataccatg gatgatctta tagtttttca aacagaagct
     1681 ggttggatag aatgttttga gcacacactt ccaatgacgg tcagtgattt cacggcctac
     1741 acctataccg atgtagatat catgcaggat acggtaacta cgttgaagtt tttggatctt
     1801 aaagtgggca ttgagcctga tgattatatc accggctaca ccatctgtga gaccaccgcc
     1861 actaccgaag tatcggcaga ggccggagca ctcaggctgt atcccaatcc tgtctccacc
     1921 acgctcagca tagacgttga gcaggcaggc tatggtaccg tgcgtgagca gacagtcgat
     1981 atcaccgacc tattaggcag gaagctatat gaggtagaag taggctatga cggccatgcc
     2041 gaagtagata caaagacgtt accaccgggc atgtatatag cagtgctacg tgcagacatg
     2101 catcaggtgc aagcagtgaa gtttgtggtg gggcggtaat gtgggctgtt taatcagccg
     2161 aataggcgta tctaatcgct gttctatggg tgtaaaatgg agcaattagt ctaattgtgg
     2221 attggttaga tgatagtaac ccacaaaatt gattcaggat atttggcaat gaaatgtcct
     2281 aagattaaaa aaatatttat ttacaattat ggaatctgat tccataattg taaatttgtg
     2341 ccatggtcaa gagagaagca gaacaggaat tacgggattt agcacaagcg tttaaagctg
     2401 tagctgttac aggaccgaga caatcgggga aaacaacttt ggttaaatat gtttttcccg
     2461 gaaaacctta tgtgaatcta gaaaatcccg atatacggct attcgcaacg gaagacccga
     2521 gaggtttctt gtccaattat ccacagggag cagtattgga tgaagtgcaa cgggtacctc
     2581 atctattttc ttttttacag gaaattttgg acaatgccac aacaaacgga atgttcgtcc
     2641 ttacggggtc taataatttt ctgttgcagg agaatatatc tcaaagtttg gcaggaaggg
     2701 ttgcgtatct gacattactt ccattgtcca ttagcgagat gaagatttcc ggaaat
//
LOCUS       DAOQQY010000336         2721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_19932_length_2721_cov_7.310578, whole genome shotgun sequence.
ACCESSION   DAOQQY010000336 DAOQQY010000000
VERSION     DAOQQY010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19932_length_2721_cov_7.310578"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..272
                     /locus_tag="PLE23_12020"
     CDS             108..272
                     /locus_tag="PLE23_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84036.1"
                     /translation="MRADTGKQYWKYRTPNYKKFPDSTFGYIKMISDPETGYLYTGDG
                     VYMMCLKPPK"
     gene            complement(356..907)
                     /locus_tag="PLE23_12025"
     CDS             complement(356..907)
                     /locus_tag="PLE23_12025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84037.1"
                     /translation="MGGYYEEDQGSGIVYPFIFRPAGTEALACMPEEPLNTQSGLEEE
                     FGYIYDATSTDIAFVWASGTMQDDSIKTSLEPFFIKKTLCNVVATSEVSKNDAPVFSL
                     YPNPASADIAVELYYEQIPLGAVLQIEIYDLLGRKHYSMPADPYSTNLIPAGTLQPGC
                     YIASLVANAKILQTLKLVVIHIP"
     gene            complement(910..1614)
                     /locus_tag="PLE23_12030"
     CDS             complement(910..1614)
                     /locus_tag="PLE23_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84038.1"
                     /translation="MLWTNNYGKLDIYDIALTNSGEIIGVATLFDPIKGFYFKMDKDG
                     KLLWARATEDFSSFNLSSVETTEDGGMIAILAIGGFGNKNLILLKINANGGFGWAKSI
                     KNINVLHAKMIKTGTDNYTIMGNSRFGIEEPPLLSFYINFDATGNITSQRFGFGDKYF
                     YPIVFNNFVAIDDQLILTSVKYDGCDVEEFRLSAINNSDKKSLWHKKLSNLKQPYVFN
                     DNFPTRSTSQDTPTVG"
     gene            complement(1648..1986)
                     /locus_tag="PLE23_12035"
     CDS             complement(1648..1986)
                     /locus_tag="PLE23_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84039.1"
                     /translation="MKKFIALIMLCKSISAMYGQQSFFNTYHPVNDMKYLNSGGIKTC
                     KNGNLVFATTYADTKVKPIKKQMVVTRVSKNGDIKWTLVLDHLTSQNWSKILEDTEGN
                     LYLPAWYKSK"
     gene            2357..>2721
                     /locus_tag="PLE23_12040"
     CDS             2357..>2721
                     /locus_tag="PLE23_12040"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX84040.1"
                     /translation="MIVTKDVCFEYNASHRWHFPDIRIDAGGSMLISGLSGSGKTTFM
                     HILAGILKPLSGKVFVNDTDIYRLSSGALDKFRGRHIGLVFQTPHFIRSLSVAENLGI
                     AMKLAGNKVHTPRIQELLT"
BASE COUNT          768 a          575 c          524 g          854 t
ORIGIN      
        1 ttggattaga gaaatccgat catatttgat ctcatagcct aatggattat ctgggataaa
       61 tactattctt aaaatgggct taagggacaa atggtactat ctatggattg cgtgccgaca
      121 cgggcaagca gtactggaaa tacagaactc ccaactacaa aaaatttcct gactctacct
      181 tcggctacat caaaatgatc agcgaccccg aaaccggcta tctctacacc ggcgacggcg
      241 tatatatgat gtgcctgaaa ccgccgaaat gatggtgtga aggacaacaa aaccggtatt
      301 tcaccgcttt ggaaggaatg tcggttttgt ttttttagca atttccgtca gcatatcatg
      361 gaatatgtat cactaccagc tttaatgttt gtagtatctt tgcgttcgct acaaggcttg
      421 caatatagca tccgggctgt agtgttcctg caggtattaa gttggtacta tatgggtcgg
      481 ccggcattga ataatgtttc cttcccagaa gatcgtatat ctctatttgc aatactgcac
      541 ccaatggtat ctgttcatag tacagttcta ccgcaatatc agccgaagcc ggattgggat
      601 agaggctgaa caccggtgca tcattcttac ttacctccga tgtagctact acattgcaca
      661 gtgttttctt aatgaaaaat ggctcaaggc ttgttttaat tgaatcatcc tgcatagttc
      721 cggaagccca gacaaacgct atgtcggtac tagtggcatc ataaatatag ccgaactctt
      781 cttccaggcc ggactgtgta ttgagcggtt cctccggcat acaggcaagg gcttcagtgc
      841 ctgcaggtct gaaaatgaaa ggatacacta taccggatcc ttgatcttcc tcgtaatatc
      901 cacccaatat caacccaccg tcggggtgtc ctgtgatgtt gaccgggtgg ggaaattgtc
      961 attaaatacg tatggttgct ttaaattact cagttttttg tgccaaagtg attttttgtc
     1021 tgaattgttt atcgctgata atctgaactc ttctacatca caaccatcat actttaccga
     1081 tgtaagaata agctgatcgt ctatagcaac aaagttatta aaaactattg gatagaaata
     1141 tttatctcca aagccgaatc tttggctagt gatattgccg gttgcatcaa aattaatata
     1201 aaaactcaac aatgggggct cttcaatccc aaatcggcta ttgcccatta tggtataatt
     1261 gtcagtaccc gtctttatca tctttgcatg tagcacgttt atatttttaa ttgattttgc
     1321 ccagccgaat ccaccattag cattaatctt caacaaaatt aagtttttgt taccaaagcc
     1381 accaattgct aatatggcta tcatacctcc gtcttctgtc gtttcgaccg aactcagatt
     1441 gaaagaacta aaatcctcgg tagcacgtgc ccataatagt tttccatcct tgtccatctt
     1501 gaagtaaaag cctttgatag gatcaaacaa ggtagccaca ccaattattt cgcccgaatt
     1561 cgtcaacgca atatcatata tgtcgagttt tccgtaatta ttagtccaca acaactgccc
     1621 gaatgaactt atttttaaga taacgcatta tttacttttg taccatgctg gcaaatacag
     1681 gttgccttcg gtgtcttcca gtattttgct ccagttttgg gaagtgagat gatcaagtac
     1741 cagtgtccat ttaatgtcgc cgttcttgct aacccgtgtt actaccattt gcttcttgat
     1801 aggctttact ttagtgtccg cataagtggt tgcaaatacc agattgccat ttttgcatgt
     1861 tttgatgcca cccgaattca aatatttcat atcatttacc ggatgatagg tgttgaagaa
     1921 tgactgctgg ccgtacatag cagaaatact tttgcacagc atgataaggg caatgaactt
     1981 tttcatgata tactgtgctg acttgttatt caatcaaaag tactatattt acatgaatta
     2041 tgcaaattat atttacataa caatccttat gaatgttgaa tattttgttg gacatcttgt
     2101 gttgtcagaa taaaatattg tttacaacga taagtatagt agggaataat aatgaatgct
     2161 acaataaatt gccgtttgcc atattttatt ttgaaattat aattttacaa aaaaagcttt
     2221 ctgccttgct tgttggggca catttcgttt tcagaaagta ttgtaatgga ttaacccaaa
     2281 ggctgtctgc gctattgcag ttcaatatac tattttaacc tatatttgca gctcagctaa
     2341 tgaattgtag ctacagatga ttgttaccaa agatgtttgt ttcgaatata atgcatccca
     2401 cagatggcat tttcccgata tccgtattga tgccggaggt agtatgctca tcagtgggct
     2461 atcgggttcc ggaaaaacta cctttatgca cattctcgcc ggaatcctaa aacctctgtc
     2521 tggcaaggtc ttcgtaaacg atacggacat ttaccggctc tcgtcaggag cactcgacaa
     2581 gtttcgcggt cgccacatag ggctggtatt tcagacaccc catttcatcc gatcgctcag
     2641 cgttgccgaa aatctcggca ttgccatgaa gctggccggc aataaagtac acactccacg
     2701 gattcaagag ctactcacca g
//
LOCUS       DAOQQY010000337         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_19946_length_2720_cov_6.658161, whole genome shotgun sequence.
ACCESSION   DAOQQY010000337 DAOQQY010000000
VERSION     DAOQQY010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19946_length_2720_cov_6.658161"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PLE23_12045"
     CDS             <1..757
                     /locus_tag="PLE23_12045"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMX84041.1"
                     /translation="IVTLDADCHPVSDNWLQTMVSQINDVHTNATTGPVRYESDGSIW
                     QNFVALDNAGMMVVTAAGYRRGWFQMANGANMCFRKEIFNKVNGYAGNVQLASGDDMF
                     LFEKIEARFPGTIRFIHTEQAIVSTQPPTDLKQWLRQRIRWGTKNRYLKNTKLKLLLG
                     FVFLINLLLMFIPVLIIFSTDPFLWVLLSVSVCLKIGSDYRLLKRGAVFLKQNSALKY
                     YPVSAILYPLLLSISGVLSMFKSRYEWKDRQVR"
     gene            complement(828..>2720)
                     /locus_tag="PLE23_12050"
     CDS             complement(828..>2720)
                     /locus_tag="PLE23_12050"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HMX84042.1"
                     /translation="EWKEAHVARVMEMAHANVNHPSIVIWSLGNEAGPGKNFKYAYDA
                     LKAFDISRPVQYERNNDIVDFGSTMYPSIEWVRGAVKGDFDIKYPFHLVEYAHSMGNA
                     CGNLKDYWDAMESTNHLFGGAIWDWIDQSMYDYDEKTGEKFLAYGGDFGDFPNDGQFV
                     MNGLIFGDYEPKPQYYEVKKVYQDIVVKEKDVQKGEFEIFNKFYFKDLSDFDVEWIII
                     KDGVTIQSGKINAGKILPRHRQSISIPYNFQELAADAEYFINIRFLQKEGKPWAKKGY
                     AQCEEQFLIKKASGWPLVSTIAAKANHAKPELMHSKGRIQTIKGDNFTILFDTETGSI
                     HSLKYGNNQIISEGKGPRINALRAFVNNDNWVYQPWFENGLHNLHHKVIRYEFTQNTD
                     GSMNAVFHVESQAPNAAKIHGGTSSGNSRIEELTDRKFGPDDFKFTSKQVYTVYADGS
                     VVLKAEINSNKPELVLGRLGFVMNIPQRYNHYTYYGRGPHDNYPDRKASSFVGVYKST
                     VADQFVNFPKPQDMGNREDVRWLSLTDDHGDGAVFIPARDNMAASALQYSPVELILAR
                     HPYLLPKSTDTYLEIDVAVTGLGGNSCGQGGPLEQDRVKAVHTELGFMISPVAKDTEY
                     PLKVRN"
BASE COUNT          729 a          619 c          535 g          837 t
ORIGIN      
        1 aatagtaacg cttgatgccg attgtcatcc cgtttctgat aattggctgc agacaatggt
       61 ttcacaaatt aatgatgtac atacaaatgc cactacaggt ccggtcaggt acgaaagcga
      121 tggcagtata tggcagaatt ttgtggcact tgacaatgcg ggaatgatgg ttgtaacggc
      181 agccggatac cgaagaggct ggtttcagat ggccaacggt gcgaatatgt gtttcagaaa
      241 ggaaatattc aataaagtaa acggatatgc aggtaatgtt cagcttgcct ccggtgatga
      301 tatgtttttg tttgaaaaaa tcgaagcacg atttcccgga acaattcggt tcatccatac
      361 agaacaagcc attgtatcga cacaacctcc gaccgatctg aagcaatggc ttagacaacg
      421 gataagatgg ggcaccaaga accgctactt gaaaaacaca aaattaaagc tgttactcgg
      481 attcgtattc ctaataaacc ttctgcttat gtttattccg gtactcatca ttttcagtac
      541 tgacccgttt ttatgggtgt tactttctgt atcggtttgt cttaaaatcg gctccgatta
      601 tcgccttcta aagcggggtg cagtatttct taagcaaaat agtgctttaa aatattatcc
      661 ggtgagcgcc atcctgtatc cgttattgct cagtatatca ggagtattat ctatgttcaa
      721 atcccgatac gagtggaaag acagacaagt gaggtaaacg aaaccactcc cgacaaatta
      781 cctcatgtaa caaaatattg ctgacgagtt ttatattgta atactcacta attacgtacc
      841 ttcaaagggt attctgtatc cttcgccacc ggactgatca taaaaccaag ctcggtatgt
      901 actgccttta cccgatcctg ctccagtggg ccaccttgac cacaactgtt gccacccaag
      961 cctgtaacag ctacatctat ctccagatat gtatcagtgg actttggcaa caaatacgga
     1021 tgccttgcca aaatcaactc aacagggctg tactgcaagg ctgacgctgc catattgtct
     1081 ctggccggga tgaatacagc tccatcacca tgatcatctg taagtgacaa ccacctgaca
     1141 tcctcgcggt tgcccatgtc ttgaggcttc ggaaaattaa cgaactgatc ggctactgta
     1201 cttttgtaca cgcctacgaa tgaagaagcc tttctgtcag gataattgtc atgtggtcca
     1261 cgtccgtagt acgtataatg gttgtaacgt tgcggtatat tcatcacaaa accaagtctg
     1321 ccaagtacta gctcaggttt gttggagtta atctcggctt taagtacaac cgaaccgtcg
     1381 gcatacaccg tatatacttg tttgctagta aatttaaaat catccgggcc aaactttctg
     1441 tctgtcagct cttctattct gctgttacct gagctggtgc caccatgtat ttttgcagca
     1501 ttgggtgcct gtgactcaac atgaaatacg gcgttcatgc tgccgtcagt gttctgtgta
     1561 aattcataac gaattacctt atggtgaaga ttgtgcagac cattctcaaa ccatggttgg
     1621 taaacccagt tgtcgttgtt tacgaatgct cgcaatgcgt tgattctagg gccttttcct
     1681 tccgatataa tctggttgtt accatacttc aggctatgta tgcttccggt ttcggtatca
     1741 aatagtattg taaaattatc tccttttatg gtctgtattc tgccttttga atgcattaat
     1801 tccggctttg catggttagc tttggctgca attgttgaaa caagaggcca cccactagct
     1861 tttttaatca ggaactgttc ttcacactgt gcatatcctt tttttgccca tggtttgcct
     1921 tccttttgca aaaacctgat gttgatgaaa tactcggcat ctgctgccag ctcctggaaa
     1981 ttatacggga tgcttataga ttgtctgtgg cgcggcaata tttttccggc atttattttg
     2041 ccactctgta tggtcacacc atctttgatt ataatccatt caacatcaaa gtctgagagg
     2101 tctttgaaat aaaatttgtt gaaaatttcg aattcgcctt tctgcacatc tttttctttt
     2161 accacaatat cttgatatac tttctttact tcgtaatact gtggttttgg ttcatagtcg
     2221 ccgaaaatga gaccattcat cacaaactga ccgtcattcg ggaaatcgcc gaaatcacct
     2281 ccgtaagcga gaaatttttc acccgttttc tcatcataat catacattga ctgatctatc
     2341 caatcccaga tggcaccacc aaacaggtgg tttgtgcttt ccattgcgtc ccagtaatcc
     2401 tttagattgc cacaagcatt acccatcgaa tgtgcatatt ctacaagatg aaaaggatat
     2461 ttgatgtcga aatcaccctt tactgcacca cgaacccact ctatagaagg atacatcgtt
     2521 gaaccaaaat caacgatatc attgttgcgc tcgtactgta caggtcgcga tatatcaaat
     2581 gcctttagtg catcataagc atacttgaaa ttctttcccg gccctgcctc attgccgagc
     2641 gaccagatta ctatagaagg atgattaacg tttgcatgtg ccatttccat tacacgtgcc
     2701 acgtgtgctt ccttccattc
//
LOCUS       DAOQQY010000338         2715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_20026_length_2715_cov_4.734962, whole genome shotgun sequence.
ACCESSION   DAOQQY010000338 DAOQQY010000000
VERSION     DAOQQY010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20026_length_2715_cov_4.734962"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PLE23_12055"
     CDS             <1..185
                     /locus_tag="PLE23_12055"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMX84043.1"
                     /translation="PDLIGRPSGFTVTVREIEVAAGANFVVAITGEMMRMPGLPEIPS
                     AEKIDIDENGVISGLF"
     gene            241..582
                     /locus_tag="PLE23_12060"
     CDS             241..582
                     /locus_tag="PLE23_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMX84044.1"
                     /translation="MATVKFSFEDKSIEPVIVNNAEEGYSILEITEDHGVHLNHNCGG
                     VCACSTCHVYIDKGMDDLEEISDKEEDFIDRAINPKLNSRLGCQCIILDAAAVIEVTI
                     PDQSRIIGHEH"
     gene            592..837
                     /gene="iscX"
                     /locus_tag="PLE23_12065"
     CDS             592..837
                     /gene="iscX"
                     /locus_tag="PLE23_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015691496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HMX84045.1"
                     /translation="MAFEDFDNLPVNWSDHEDIAMGLYEKFGDEFGESKIYRIRFTDL
                     LEWVLSLPNFVGKREDCNEAHLELIQSQWVYEWRANQ"
     gene            853..1353
                     /locus_tag="PLE23_12070"
     CDS             853..1353
                     /locus_tag="PLE23_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HMX84046.1"
                     /translation="MPDLNIFRPITTFILDVDGVMTDNRILVTENGECLRNMNIRDGY
                     AIRRAIWKDYRFFVITAGKSDGVVKRLQDLGVEHIYTQTREKIKVYESIISEFELDEN
                     AVLYMGDDMPDLEVMQRVGCPVCPADAIQEIKAVSAYISPMDGGDGCVRDVIEKVLKL
                     NGHWID"
     gene            1355..2281
                     /locus_tag="PLE23_12075"
     CDS             1355..2281
                     /locus_tag="PLE23_12075"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HMX84047.1"
                     /translation="MKSPIGILKTFRIGNLLIIALLEFILHRSIISSALYKSGISPAL
                     SEANLGILIVFSMFLTWSGYLINDIYDYDIDRINKSGFSQVDYFGGKNGSWKAYVILS
                     LFCLLLAIYLSVTLGKWNYFWLLPLAMLTLWLYSSKLKNHFFLGNIGVSVLVCLVILL
                     VWLSENTQIGLISDISVKVRLSGIFLLYAAFAFLTTWARELVKDAQDLEGDRHNNLQT
                     FPARYGTKPTMHYLIILLSGLLTMMCLVFYINIGIFSNFQILFAIFFLILPVLLVMGL
                     AVKAWRKSDFGRMSFLLKLTMLAGIIFLLTLP"
     gene            2292..>2715
                     /locus_tag="PLE23_12080"
     CDS             2292..>2715
                     /locus_tag="PLE23_12080"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HMX84048.1"
                     /translation="MKIILASKSPRRKELLLQAGFDIEIFHKEVDESFPESMPVEDVA
                     EYLAVKKALACTEILTDDKILLSADSVVILNGKIYGKPTDAEDAVRILKELSGNEHTV
                     ITGVCLKTPSKQLSFSGISKVRFGRLSDDEIRYYITTCR"
BASE COUNT          809 a          513 c          568 g          825 t
ORIGIN      
        1 atccggatct tataggccga ccaagcggtt ttacggtgac agttagagaa attgaagtgg
       61 cagcaggtgc aaatttcgta gtagccatca ccggtgaaat gatgcgaatg cccggcttac
      121 cggaaattcc gtcagcagaa aagatcgaca ttgatgaaaa tggtgttatc tccggtttat
      181 tttaattttg aacattttta taatacagct tgttgaatat ctgaatctta acatgcaata
      241 atggcaacag taaaatttag ctttgaagat aaatcaatag aacctgtaat tgtaaataat
      301 gctgaagaag gctactcaat tctcgaaata accgaagacc acggtgttca tctcaatcat
      361 aactgtggcg gtgtctgtgc atgcagtact tgccatgttt acatagacaa aggaatggac
      421 gacttggaag aaatatctga caaagaagaa gatttcattg atcgggccat caatcctaag
      481 cttaattcaa gattaggctg ccagtgtatc atccttgatg cagctgcagt tattgaagta
      541 accatcccgg atcaaagcag aattatagga cacgaacatt aaaattaaag tatggcattt
      601 gaagattttg acaacctgcc ggtcaactgg tctgaccacg aagatatagc aatgggcctt
      661 tatgaaaaat ttggtgatga gttcggagaa tccaaaattt acagaatcag gtttactgat
      721 ctgttagaat gggtactttc tctacctaac tttgtaggta agcgtgaaga ctgtaatgaa
      781 gcccatctag aattaataca gtcgcagtgg gtttacgaat ggcgagctaa ccagtaaaaa
      841 attgatcgac acatgcctga ccttaatatt tttcgcccaa ttaccacatt tattttggat
      901 gttgatggcg tcatgaccga caataggata ctcgttaccg aaaatggtga atgcctccgc
      961 aatatgaata ttcgcgatgg atatgccatc cgtcgtgcta tctggaaaga ttacagattt
     1021 ttcgttatca cagccggtaa gtcagacggt gtagtaaaaa ggcttcagga tttgggcgtt
     1081 gaacatatct atacccagac aagagaaaag ataaaggtgt acgaatcgat tatttctgaa
     1141 ttcgaactgg atgaaaacgc tgttttgtac atgggtgacg acatgcccga cctcgaagtg
     1201 atgcaacgtg tcggatgccc ggtatgccct gcggatgcca tccaggagat aaaagctgtc
     1261 tctgcttata tttcacctat ggatggtgga gacggttgtg taagagacgt cattgaaaaa
     1321 gtgctcaaac tcaacgggca ctggatagac tgaaatgaaa tcaccgattg gtattcttaa
     1381 aaccttcagg attggcaatc tgctgatcat tgcattatta gaattcattc ttcatcggtc
     1441 aataattagt agtgctcttt ataagtcggg tatttcaccg gcattgtcag aagccaactt
     1501 gggtattctc atcgtattca gtatgtttct tacctggtca ggatatttga ttaatgatat
     1561 ttacgactac gatattgaca ggattaacaa gtcaggtttt agccaagttg attattttgg
     1621 tgggaaaaat ggctcgtgga aagcatatgt aatactttca ttattctgcc tgttacttgc
     1681 catttatctt tcagtaacgc tcggcaaatg gaattatttc tggttattac cgctggcaat
     1741 gcttactctt tggctttatt cgtcaaagct aaaaaatcac ttttttcttg gcaacatcgg
     1801 agtatctgtc cttgtatgtt tagtaatact tttagtctgg ctttcagaaa atactcagat
     1861 tggtctgata tcagacatca gtgtcaaagt ccgtcttagt ggtattttct tattgtatgc
     1921 tgcattcgca tttcttacaa catgggcacg cgaactggtc aaagatgctc aagatctgga
     1981 aggtgaccgg cataataatc ttcagacatt ccctgccaga tatggtacca aaccaaccat
     2041 gcattatctc attatccttt tgtcaggttt actaacgatg atgtgtcttg tgttttatat
     2101 caatattggc atcttttcca attttcaaat tctgtttgcc atatttttcc tcatattacc
     2161 ggtacttttg gtaatgggtc ttgcagtcaa agcctggcga aaatcagatt ttggcaggat
     2221 gagcttcttg ttaaaactta ccatgcttgc aggaataatt ttcttactca cattgcccta
     2281 accacacacc tatgaagata atacttgcct ccaaatcacc acgaagaaaa gaactcctgt
     2341 tacaagcagg ctttgacata gaaatatttc acaaagaagt agatgaatcc ttccctgaaa
     2401 gtatgcctgt tgaagatgtt gccgaatact tggctgtaaa gaaagcactc gcatgtacag
     2461 aaatattgac tgatgacaaa attctcttat cggctgacag tgttgtaatt ctgaatggga
     2521 aaatatatgg caaacctacg gatgcagaag atgctgtacg gatcctgaag gagttatccg
     2581 gcaacgaaca tactgtcata acaggtgttt gtctgaaaac tccatccaaa cagcttagtt
     2641 tttcgggaat ctctaaggta agatttggcc ggttgtctga cgatgaaatc cgttattaca
     2701 ttactacctg ccgac
//
LOCUS       DAOQQY010000339         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_20053_length_2713_cov_5.936042, whole genome shotgun sequence.
ACCESSION   DAOQQY010000339 DAOQQY010000000
VERSION     DAOQQY010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20053_length_2713_cov_5.936042"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="PLE23_12085"
     CDS             <1..436
                     /locus_tag="PLE23_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84049.1"
                     /translation="VMPYTYTHADSTASYTHYNQALAHPLGANLVEGIARVKYRILPN
                     LQAECWLQHYKQGLDSNTSNWGENILTTNTSRVSDYGNKLLQGLRTNVTRLTFRLEWK
                     FFHNMNAFGEYSYRRQTGYNEQTLHYAGAGFKINIGSKPLLL"
     gene            complement(460..1275)
                     /locus_tag="PLE23_12090"
     CDS             complement(460..1275)
                     /locus_tag="PLE23_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMX84050.1"
                     /translation="MEKYIDNLLIKAVEFAPKLAMALITLFVGFWLINRLTRLMVAVM
                     KKRELDETVVPFLESIVTVILKALVLVSVAGMFGIETTSFVTILGAAGLAVGLALQGS
                     LGHFASGILILLFKPYKVGDTIRVKEFEGVVEEIQVFNTLLRTNNNKKIIIPNGVITA
                     DPIINITGQGTLRVVADFVVAYEEDIDKVRKIILEVIRSNERIFNDPAPEVQYNTKGF
                     GLAVYECTFWCHAEEQNQVYYFVQEHVRKSFDRNGIKTPEIGFEYGSTKGKTI"
     gene            complement(1293..2168)
                     /locus_tag="PLE23_12095"
     CDS             complement(1293..2168)
                     /locus_tag="PLE23_12095"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HMX84051.1"
                     /translation="MKFISYIFIRLLVFIFSILPFRVLYLFSDFIKWVLKDLIKYRTH
                     VVEDQLRKCFPHMSDQEIENIKDENYRNLADLVVESIKGFSMSEDALKTRWKLINAEK
                     IEAFLKQGRPVIVNTAHYNNFEWGTLAFALQSHYPVKGVYKPLTNHFIENYLKRNRAK
                     TGMELLPMQNVGQKISQFKEQASIFIFISDQSPSKVEKAHWINFFGHETAFIHGVGQY
                     SSEYNIPVFYCDIQRVKRGYYEATLTELVAEPKLSSAYEITRTFAAKVEQVIVQKPAP
                     WLWTHKRWKHRRYPE"
     gene            2385..>2713
                     /locus_tag="PLE23_12100"
     CDS             2385..>2713
                     /locus_tag="PLE23_12100"
                     /inference="COORDINATES: protein motif:HMM:NF037611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84052.1"
                     /translation="MRSFFTFISFIFLANFTIAQPSATKLTSYVDPFIGTGGTGHTFP
                     GATLPHGMVQLSPDTRTEGWESCAGYYFPDREILGFSHTHLSGTGVPDLGDLLILPLT
                     GTAKIKK"
BASE COUNT          813 a          589 c          506 g          805 t
ORIGIN      
        1 ggtgatgcca tatacttata cacatgccga ttcgaccgca tcttatacac attataacca
       61 agcattggca cacccacttg gagccaacct tgtcgagggt atcgcaaggg tcaaataccg
      121 aatattgcca aatctgcagg cagaatgctg gctgcaacat tacaaacaag gtcttgacag
      181 caacacatct aactggggcg aaaacatcct caccaccaat acaagccgtg tgagcgacta
      241 cggcaataag ttattgcagg gactgcgcac caatgttacc cgcttgacat tcaggctcga
      301 atggaaattt tttcacaaca tgaatgcatt tggagaatac agttatcgaa ggcagacagg
      361 atataacgaa caaacgctcc attatgccgg tgccggattc aagattaata ttggaagtaa
      421 gccgctattg ttgtaataaa ggaattcgct tacaacagct taaatagttt ttcccttagt
      481 actaccgtat tcaaagccta tttcaggagt tttaatacca tttcggtcaa atgatttgcg
      541 tacgtgctct tgtacaaaat aatatacctg gttctgttct tcggcatgac accagaaagt
      601 acattcatac actgccagcc caaaaccctt tgtgttgtat tgcacttcag gtgccggatc
      661 gttgaatata cgttcgtttg agcggatgac ttccaggatg attttcctga ctttgtcaat
      721 atcttcttca taagcaacta cgaaatctgc cactaccctt agcgtgcctt gccccgttat
      781 gttgataata gggtcagcgg ttatcacacc atttggtata atgatttttt tattgttatt
      841 ggtgcggagc aatgtgttga atacttgtat ttcttcaacc acaccttcaa actccttcac
      901 tcgtatggta tcacctacct tatatggttt aaacaacaga atcaaaatac cactggcaaa
      961 atgcccgagg ctaccctgca atgcaagacc tacagcaaga cccgcagcac caagaatggt
     1021 aacaaatgag gtggtttcga taccaaacat gccggccaca cttaccagca ccaaagcttt
     1081 aaggatgacc gtcacaattg attctagaaa aggaaccact gtttcatcga gttcgcgttt
     1141 tttcattact gctaccatga gccgtgtaag cctgttgatg agccaaaacc caacaaacag
     1201 tgtaatgagt gccatagcaa gttttggtgc aaactcgacg gcttttatca ataaattgtc
     1261 aatatatttc tccataacga atattagcta ttttattcag gatatcgcct gtgtttccat
     1321 cttttgtgtg tccagagcca gggtgcaggt ttttggacga tgacctgttc tactttggca
     1381 gcaaatgtgc gtgtgatttc ataagctgat gatagcttag gttctgccac caactctgta
     1441 agcgtagctt cataataacc acgttttacc ctttggatgt cgcaataaaa aacaggaatg
     1501 ttgtattcac ttgaatattg gcctacacca tgaatgaatg ccgtctcatg cccaaaaaaa
     1561 ttaatccagt gagctttctc cactttggat gggctttggt cagatataaa aataaagata
     1621 cttgcctgtt ctttaaactg tgatattttt tgtcctacat tctgcattgg caacaactcc
     1681 attcctgtct ttgctctatt cctttttaaa taattttcaa taaaatgatt ggtcaatggt
     1741 ttataaaccc cttttaccgg ataatgtgac tgaagtgcaa atgctagtgt cccccattcg
     1801 aagttgttat aatgagcagt attaacaatc actggtctgc cttgtttgag aaatgcctct
     1861 attttttctg cattgattag cttccatctg gtcttcaacg catcttcact catcgaaaag
     1921 cccttgatgc tctctacaac caggtctgcc aggttgcggt aattttcatc cttgatgttc
     1981 tctatttcct gatctgacat gtgtggaaaa cattttctaa gctgatcttc tactacgtgg
     2041 gtacggtatt ttatcaggtc ttttaatacc catttgataa aatcagaaaa caaatacaat
     2101 acccggaatg gcagaataga aaagataaaa accaaaagac gaataaaaat atacgaaata
     2161 aacttcataa tgtaggaatg actgccaaaa ttagtaattt taacccagat aacgcattag
     2221 gaatttaaac gaaaatgatt cgtacaaaaa attcttaaag aagtaataga atgcatagtg
     2281 acctatgttt tttgagcttt tggaatggat ttaagtaatc attatgtgca gtaaaaaccc
     2341 taatgcgtat ctgagttaaa catgcaaaat tcaatttaag aatcatgaga agttttttca
     2401 cttttatctc ttttatattt ctggcaaatt tcaccattgc acaaccatct gctaccaagc
     2461 tcacctctta cgttgacccc tttataggta caggtggcac aggacacact ttcccgggcg
     2521 cgaccctgcc acacggaatg gttcaactga gcccggacac aagaacagaa ggatgggaaa
     2581 gttgcgccgg ttattatttc cctgaccggg aaatactcgg attcagccat actcacctga
     2641 gtggcaccgg agtaccagat ctgggagact tactcatatt gccattgaca ggtactgcca
     2701 aaatcaaaaa ata
//
LOCUS       DAOQQY010000340         2686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_20404_length_2686_cov_4.568605, whole genome shotgun sequence.
ACCESSION   DAOQQY010000340 DAOQQY010000000
VERSION     DAOQQY010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20404_length_2686_cov_4.568605"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1762
                     /gene="acs"
                     /locus_tag="PLE23_12105"
     CDS             <1..1762
                     /gene="acs"
                     /locus_tag="PLE23_12105"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMX84053.1"
                     /translation="ITPDVRWFEGGRLNITENCLDRHIADKGDKPAIIWEPNDPTGPS
                     ISISYRQLLAQVNQCANALRSMGVSKGDRICIYMPMIPQAVVAMLACARVGAVHSIVF
                     AGFSAKSLSDRITDTGCKWVICSDYNRRGAKDIAVKEVVDEALSLEPNLVEKVLVYKH
                     TNKTTCWNPELDVCWQDIVPLMPEKCAPEIMDSEDMLFILYTSGSTGKPKGAVHTIGG
                     YMVFTCFTFINTFQYKNDDIYWCTADIGWITGHSYGVYGPLLAGATTLMFEGVPNYPD
                     AGRFWQIIDKYRVTQFYTAPTAIRSLMASAWDEPDKYKLDSLKVIGSVGEPINEEAWH
                     WYDEKVGRGKVPIVDTWWQTENGGIMITPLPGITDTKPCYASYPLPGVQPVIVNQDGK
                     ILEGNDVEGLLCIRFPWPSVVRTTYGDHERCRTTYFAPFPGFYFTGDAARRDSEGRYR
                     IMGRVDDVINVSGHRLGTAEVEDAINSHPEIVESAVVGFPHPIKGQGIYAYVTALHDV
                     EDKEKLIREVLQVVTEQIGAIAKPDKIQFAPQLPKTRSGKIMRRILRKIAEGAPEQIG
                     DTSTLINPECVQDLIDGCEL"
     gene            1880..2596
                     /locus_tag="PLE23_12110"
     CDS             1880..2596
                     /locus_tag="PLE23_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMX84054.1"
                     /translation="MLKAIPHILTLSNLFFGCAAVVSLFTGNWNASVIFVFMSGVADF
                     LDGLAARALHVAGELGKQLDSLADVVSFGLVPGLIAYILILRSLGIQYEPIQALHWQA
                     MPGFLITLFSALRLGKFNIDTRQTHEFIGLNTPTNTFFFCGLLWLAGDGYLSQVDTPI
                     FWIVLSVVMSLLLIAPIPMFNFKTTTLGFKGNEIRILFLIGVVLFVIFFGKAALSLAV
                     LYYILLSFIQFLFKKNKTTI"
     gene            2593..>2686
                     /locus_tag="PLE23_12115"
     CDS             2593..>2686
                     /locus_tag="PLE23_12115"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMX84055.1"
                     /translation="MKFLAEIEIMPHKELLDPQGKTVAKNLKYIN"
BASE COUNT          721 a          565 c          658 g          742 t
ORIGIN      
        1 cataacaccg gatgtaaggt ggtttgaagg tggcaggctc aatattacgg aaaactgcct
       61 cgacagacac atagcggaca aaggcgacaa gcctgccatt atatgggaac caaatgaccc
      121 gacagggcca agcattagta taagctacag acaattactg gcacaggtaa atcagtgtgc
      181 caatgcgctg cggtcaatgg gtgttagtaa aggagaccgt atatgtatct atatgccgat
      241 gataccacag gctgttgttg ctatgttggc ttgtgcacgt gttggtgctg tacactccat
      301 tgtgtttgca ggattttctg ccaaatcact ttctgaccgt ataacggata ccggatgtaa
      361 atgggtcatc tgcagcgact acaacagacg tggtgctaaa gacatagcag tgaaagaagt
      421 agtagatgaa gccctctctc ttgaaccaaa tctggtagag aaggtgctgg tgtacaaaca
      481 taccaataag actacctgct ggaatcctga actcgatgtt tgttggcagg atatagtacc
      541 acttatgcca gaaaaatgtg cgccggaaat catggactca gaagatatgc tctttatttt
      601 atatacttca ggttctaccg gtaagccaaa aggtgcggta catacgattg gtggctatat
      661 ggtttttaca tgttttacct ttatcaatac atttcagtat aaaaatgatg acatttactg
      721 gtgtacggct gatataggct ggattacagg gcattcgtac ggtgtttatg gtccgttgct
      781 ggccggtgcc acgaccctga tgttcgaagg tgtacccaac taccctgatg ccggacgttt
      841 ctggcagata atcgataaat atcgggttac acagttttat acagcaccaa cggctatccg
      901 ttcattaatg gcttcagcat gggacgaacc tgacaaatat aaacttgaca gtctcaaagt
      961 aattggttcg gtcggcgagc caatcaatga agaggcctgg cattggtatg acgagaaagt
     1021 aggccgtggg aaagttccga ttgtggacac ctggtggcag accgaaaatg gtggaatcat
     1081 gataacacca ctacccggca ttacagacac caagccatgc tatgcgtctt atccgctacc
     1141 tggtgtacag cctgtcatcg ttaaccagga cggaaaaata ctcgagggaa atgatgtaga
     1201 aggcctgttg tgcatacgtt ttccatggcc gtctgtagta agaacaactt atggcgacca
     1261 tgagagatgc cgaacaactt attttgcgcc atttccgggc ttttatttca caggcgatgc
     1321 tgcccgacgt gattcagaag gcagataccg tatcatgggt cgtgtggacg atgttatcaa
     1381 tgtgtcaggg caccggcttg gtaccgctga ggtagaagat gccatcaaca gccatccgga
     1441 aatcgtcgag tctgcggtag tcggatttcc gcatcctatt aaagggcaag gcatttatgc
     1501 ttatgtgacg gcactgcacg atgtggaaga caaagaaaaa ctgatccggg aggtgctgca
     1561 ggtggtgaca gaacagatag gcgccatagc caagccggat aaaatccagt ttgcaccaca
     1621 actgccgaag acccgttccg gcaaaatcat gcggcgcata ctgagaaaaa tagctgaagg
     1681 agcacccgaa cagattggtg acacatcgac tttgatcaat ccggagtgtg tgcaggattt
     1741 gattgatggg tgtgaacttt gacggtttga tttgcattat tttttggtgc gccggaaagc
     1801 ttaagcaaat ttttgtagaa ataaatttct tcccgccaat cttcacggca taagcgtata
     1861 taagactatc tttgtgccga tgttgaaggc tataccacat atacttacct tatcgaatct
     1921 gtttttcgga tgcgcagctg ttgtgtctct gtttacgggc aactggaatg catcggtcat
     1981 ctttgtgttt atgagtggtg tggctgattt cctggacgga ttggctgcac gggcacttca
     2041 tgtggcagga gaacttggca aacaactcga ttcgctcgct gacgtcgtga gttttgggct
     2101 tgtaccgggg ctgattgcct atattctgat attgcgctct ttgggcatcc aatatgaacc
     2161 catacaagcg cttcactggc aggcaatgcc gggattcctc atcacgttgt tcagtgcgct
     2221 gcgccttggg aaattcaaca ttgatacaag acaaacccac gaatttattg gcctgaatac
     2281 gcctactaat acattctttt tctgcggctt gctatggctg gcaggagatg gttatctgag
     2341 ccaggtcgat acgcccatat tttggattgt gctttctgtt gtcatgtctt tgttgttgat
     2401 agctccgata cccatgttta atttcaagac tacgacactc ggatttaaag gaaatgagat
     2461 acgcattttg tttcttatcg gagttgtttt gtttgtgata ttttttggaa aagcagcctt
     2521 gtcgcttgct gtactgtact atattttact ttccttcatc caatttttat tcaagaaaaa
     2581 taaaaccaca atatgaagtt tttagccgaa atcgaaatca tgcctcacaa ggaactgctc
     2641 gatccgcagg gtaaaaccgt agccaaaaat ctgaaataca tcaaca
//
LOCUS       DAOQQY010000341         2678 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_20502_length_2678_cov_5.923751, whole genome shotgun sequence.
ACCESSION   DAOQQY010000341 DAOQQY010000000
VERSION     DAOQQY010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20502_length_2678_cov_5.923751"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(565..1731)
                     /locus_tag="PLE23_12120"
     CDS             complement(565..1731)
                     /locus_tag="PLE23_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HMX84056.1"
                     /translation="MATPTILPPSKLPNVGTTIFTTMSALANEYSAVNLSQGFPDFMC
                     SEKLINLVSKAMHDGKNQYAPMAGDPLLRQRIAHKVESLYRQSIDPDTELTITAGGTQ
                     AIFTAIGAFVHPGDEVIIIEPAYDCYKPAIELFGGHTVPYALHAPDYTIDWAVLESLI
                     TPATRMLITNNPHNPTGRVFSRHDLTRLRDIVIRHNLLLISDEVYEHLTYDGLTHESV
                     LRYPELYARTLVIFSFGKTYHNTGWKTGYCIAPPGLTTEFRKVHQFNVFSANTPIQAA
                     YSAFMADPNEYLGLPAFFQAKRDLLLHSMQGSAFSPLICQGSYFQLFDYSSLSDLPDT
                     EFCQWLVQHHQVAAIPLSPFYTVPPDDKIIRLCFAKKDETITNAAKRLRDILIM"
     gene            complement(1750..2004)
                     /locus_tag="PLE23_12125"
     CDS             complement(1750..2004)
                     /locus_tag="PLE23_12125"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMX84057.1"
                     /translation="MWTENDKQLSISVSFTDFSSAWAFMTEVALVAEKAGHHPDWHNV
                     YNRVTIRLSTHEAGNTVTQKDRDLASAIDHILSRYTHQLL"
     gene            complement(2011..>2678)
                     /locus_tag="PLE23_12130"
     CDS             complement(2011..>2678)
                     /locus_tag="PLE23_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMX84058.1"
                     /translation="ITDYYIDMGMSKDDLVKHISIGDPITRERDTIEMGDCINAKSLD
                     NRISVFILIETLKELARTELPCSLYAVFTVQEEVGLRGVSPAAHSIEPDYGINIDTTI
                     AFDTPGAQAWESITHLGQGTAIKIADGSVICDTRMVSFMKYMADKHGIPWQPEILTAG
                     GTDTAGLQRSGKGGSISGAISIPTRHIHSVIEMVHKTDVLNSIRLLKNCITEISNFNW
                     NHK"
BASE COUNT          625 a          532 c          772 g          749 t
ORIGIN      
        1 acctaatagt tctaccatct acggatacta ttttgtaaac cgataatttt ttcatgatac
       61 aatattatca gtcaaacata aggttgaata cattcataac gtgctgatta tgttgatgaa
      121 aggattattt accttaataa tacgattata gtgtaaatta atcgtaagtt tacggtaaaa
      181 gtgtgtttac tgctgtgtca agtaggcttg tcttcgagac aatctacttt ggcccaaaag
      241 ctcaaaaaaa cctagttcac tgcgcttcta ctcctttttt atcgatcttt tgtgctcatc
      301 acttttttca aaattcctat tgcgttatcc gggtattata aatctactgc ttaatggttc
      361 gaaaaatgcc gaaaaataat gtgtcacaac atctcacatt ttacacacca ttcaccactt
      421 accacttacc attcaccata gttgcgtgaa ggacagaagc gaacacgaag taaagcggat
      481 ggcctgaccc gacaggggca cgccctaata atttgaaaca cgacagttaa gtgtatggtg
      541 actgtgtagt atattcaaag cagcttacat gatgagtatg tcgcgaaggc gttttgcggc
      601 gttggtgatg gtttcgtcct ttttggcgaa gcataggcgg atgatcttgt cgtcgggcgg
      661 aacggtgtaa aatggtgaaa gtggtatggc tgcaacctgg tgatgttgta caagccattg
      721 gcagaattcg gtatcgggca ggtcgctgag gctgctgtag tcgaagagtt ggaagtatga
      781 gccctgacaa atgagcggac tgaaggcgct gccctgcatg gaatgaagca ggaggtctcg
      841 tttggcctga aagaaggccg gcagaccaag gtattcattg gggtcggcca tgaatgcgga
      901 atatgctgcc tgaatgggtg tattggcact gaaaacgttg aactggtgta ctttgcgaaa
      961 ctcggtggtg agaccgggag gtgctatgca gtagccggtt ttccagcctg tgttgtgata
     1021 ggtcttgccg aatgagaata taaccagcgt gcgtgcatac agctcgggat agcggaggac
     1081 gctctcgtgc gtgaggccgt cgtaggtgag gtgctcatac acttcgtcgg aaatgaggag
     1141 gaggttgtgc cggatgacga tatctcgtag gcgtgtgagg tcgtgccggg agaaaacccg
     1201 tccggtaggg ttgtgcggat tgttggttat gagcatccgg gtagccggtg tgatgagtga
     1261 ttcgagtact gcccagtcga tggtgtagtc gggagcgtgc agagcataag gcacggtatg
     1321 gccgccgaag agttcgatgg cgggtttgta gcagtcataa gctggctcga tgatgatgac
     1381 ttcgtcgccc gggtgtacga aagcaccgat ggcagtaaag atggcctgag taccgccagc
     1441 cgtaatggta agctctgtgt cggggtcaat gctctgccta tagaggcttt cgaccttgtg
     1501 tgcgatacgt tgtctaagca gtgggtcgcc agccataggt gcatactggt ttttgccatc
     1561 gtgcatggct ttgctgacca ggttgataag cttttcggag cacatgaagt ccggaaaacc
     1621 ctgagagagg tttacagcgc tgtattcgtt ggcaagggct gacatggtgg taaatatggt
     1681 agtgcctaca ttgggtagtt tggaaggcgg aaggatagtt ggggttgcca tagcgagtga
     1741 agtgtgtgat tagagtagct gatgcgtata tcttgacagg atgtggtcaa tagcagaggc
     1801 caggtcacgg tccttttgtg tgacggtatt gccggcttcg tgtgttgaaa ggcgaatggt
     1861 gacacggtta tacacattgt gccagtctgg gtggtgaccg gctttctcgg ccacgagtgc
     1921 tacttcggtc atgaatgccc atgcggaaga aaagtccgtg aagctgaccg aaatgctgag
     1981 ttgtttgtcg ttttctgtcc acatggtgct ttatttatgg ttccagttaa aatttgaaat
     2041 ttcggtgatg cagtttttga gcaggcgtat gctgttgagt acatcggttt tgtgtaccat
     2101 ctctataacg gaatgtatgt gccgtgtagg gatggagatg gcaccgctga tactgccgcc
     2161 cttacccgaa cgttgcagac ctgccgtgtc tgtaccgcct gcagtgagta tttcgggttg
     2221 ccatggtatg ccatgtttgt cagccatgta cttcatgaaa ctgaccatac gggtgtcgca
     2281 gatgactgaa ccgtcggcaa tcttgatagc tgtaccctgg ccaagatggg tgatagactc
     2341 ccatgcttgt gcgccaggag tgtcgaatgc gatggtggta tcgatattga taccgtagtc
     2401 gggctctata ctgtgtgctg ccggagacac accacggagg cctacttctt cttgcactgt
     2461 aaatacagca tacagggagc atggtagctc ggtacgtgcg agctctttga gtgtctctat
     2521 caatataaag accgagatgc ggttgtctag tgatttggca ttgatgcagt cgcccatttc
     2581 gatggtatcg cgttcgcgtg tgatagggtc gcctatgctg atgtgtttga ccaggtcgtc
     2641 tttgctcata cccatgtcga tgtagtaatc tgtaatgg
//
LOCUS       DAOQQY010000342         2665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_20661_length_2665_cov_4.834100, whole genome shotgun sequence.
ACCESSION   DAOQQY010000342 DAOQQY010000000
VERSION     DAOQQY010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20661_length_2665_cov_4.834100"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="PLE23_12135"
     CDS             complement(<1..792)
                     /locus_tag="PLE23_12135"
                     /inference="COORDINATES: protein motif:HMM:NF024972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84059.1"
                     /translation="MKKISLFLFLLLLLGLQANAQKNFWTYTTPEKVVIPENESQWIV
                     PKKFATLELDFNSFKKSVWNAPMEFTSSRGKLISIPMPDGSIKEFEVFQSPCMQEGLS
                     AKFPNIRSFAGYNPENKLDRIRLDISHRGVKCIITSNGKQVYIDPFARKQTKYYIAYY
                     TSDYEKDPNGVKFVCGLDDEVAKQGLTNDYSPATEEQLKTLALKQRTSTLPLNKYRIA
                     IATTGLYSQFIGTTVEDVIAELNTAVNRINEVLTNDLSVKFELVND"
     gene            complement(885..>2665)
                     /locus_tag="PLE23_12140"
     CDS             complement(885..>2665)
                     /locus_tag="PLE23_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84060.1"
                     /translation="YAETEFVNKNFSPLNPYRNAEFNRDYNYKPLNVDEWWINAGIQL
                     TKSNTFSLKYTYNSFSQSNLFKANKHDFSNRYVKKTWTSILESSYLLTSGNQEKSWFL
                     RPKYELSKTLLSTQDLTLKIFADGESNERRSLQADTLLATSFRYINMGAGVSYKPVSK
                     LNSSFNFSRRIDDLPVKNEFKMLSVADQFGLENNFKIKKNQTVQLIAQYRILNVTSVK
                     TNLKNAESYLGRFQHNIRTKDGSMSNLLSYEIGSGQEQKIDYYYQEVSPGLGQYEYND
                     YNGDGVKQLDEFEIATSVDKAKFVRLIVLSNDFVKTDNLSITENLSLGFPSAWSNAGV
                     FKKALQKLNWVSSVQFNRKLKESKISQVWNPFSAFDEVSQLALLGYFYSSVLQVNRGK
                     PGYELQFGTNGTSNKQLLTSGLEGRTTKEFFQFTRINLSPKWQFENRMSVSSIVSTAD
                     LFKSRNFILQVQKTEPAIILNSSLHTRIALKGKWEAGKTNETVNGVSSDAKQISAEVR
                     HNTSNRSSLNGKFTFTDIKFEGNQSSPAGFALLQGLNNGKNLQLNVDIDYRLTQKLFL
                     NVGYEVRKLGDSKLIHVFRTQLKADF"
BASE COUNT          691 a          506 c          543 g          925 t
ORIGIN      
        1 atcatttaca agttcgaatt taacagacag atcgttggtg agtacctcat tgatacgatt
       61 gacagctgtg tttaactccg caattacatc ttctacggta gtgccgatga attgagaata
      121 aagcccggtg gtggctatcg caatgcggta tttattcaaa ggaagggttg atgtccgctg
      181 ttttagtgcc agcgttttga gttgttcctc tgttgccggg ctataatcat tggtcaggcc
      241 ctgtttggca acttcatcat ccaatccaca cacaaacttt accccatttg ggtctttttc
      301 atagtctgaa gtataatagg ctatataata ctttgtctgt tttctcgcaa atggatcaat
      361 atacacctgc ttgccgttgg aggtaatgat acattttaca ccgcggtgcg aaatatctag
      421 gcgaatgcgg tcaagcttgt tttcagggtt gtatcctgca aaactcctta tgttagggaa
      481 ttttgctgaa agaccttcct gcatacaagg tgattggaat acttcgaatt cctttatgct
      541 gccatcaggc atcgggatgg aaatgagttt tcctcttgaa ctggtaaatt ccattggtgc
      601 attccagaca gattttttga atgaattaaa atctagctcg agtgtggcaa actttttagg
      661 tacaatccac tggctctcat tttcaggtat tactaccttt tcgggggtag tgtatgtcca
      721 gaaattcttt tgtgcattgg cttgtaggcc tagcaataat aataagaata aaaagagtga
      781 gatttttttc atttaggcgt ttttttgagt tggcaaaggt atgtaaataa cactttaact
      841 taaaaattgg ttttgttaaa aaaatgcgga atttggaaaa taacttaaaa atctgctttt
      901 aattgtgttc tgaacacatg tatcagtttc gaatcaccaa gttttctgac ttcatagccc
      961 acattcaaaa atagtttttg ggttagcctg taatctatat ctacattaag ttgcaggttt
     1021 tttccattgt taagcccctg aagcaatgca aagccggctg gcgaactctg gttgccttca
     1081 aatttgatat ccgtaaatgt aaattttcca ttcagtgaag atctgttgct tgtattatgc
     1141 ctcacttctg ccgatatctg tttggcatca ctacttactc cattgactgt ttcattggtc
     1201 ttgcctgctt cccattttcc tttaagtgct atcctggtat gcaggctcga attgaggatg
     1261 atagcaggtt ctgttttttg cacttgcaat ataaaattcc gcgacttgaa caaatcggca
     1321 gtacttacaa tgctgctgac cgacatacgg ttttcgaact gccacttagg gctgaggttt
     1381 attcttgtga attgaaaaaa ttccttcgtt gttcttcctt caagtccgga tgtaagcaat
     1441 tgtttgtttg atgtgccgtt ggtgccaaac tgtagctcat aaccaggttt gccacgattg
     1501 acctgcaata ccgagctata gaaatacccg agcaaggcaa gttggctgac ttcatcaaat
     1561 gcagaaaatg gattccagac ctgactgatt tttgattctt tgagcttacg attgaactgt
     1621 actgaactca cccagttgag tttttgaagg gcttttttga atacaccggc attcgaccac
     1681 gctgaaggga aacccagtga gagattttct gttatggata gattgtcagt ttttacaaaa
     1741 tcatttgata atactatcaa ccgtacaaat ttagctttat caaccgatgt ggcgatttcg
     1801 aattcgtcaa gctgttttac accatctccg ttgtagtcgt tgtattcgta ctgtccgagt
     1861 ccggggctta cttcctggta gtaatagtcg attttttgtt cttgaccaga cccaatttca
     1921 taactcagca gattgctcat gctgccatct ttggtgcgaa tattgtgttg gaatctacct
     1981 aaataactct ctgcattttt aaggtttgtt ttgactgacg ttacattcag tatgcggtat
     2041 tgtgcaataa gctgtacggt ctggtttttt ttaattttaa aattattctc aagaccgaac
     2101 tggtcagcaa cactcagcat cttaaactcg tttttgaccg gtaggtcatc gattcttcta
     2161 gaaaaattaa agcttgagtt cagttttgat actggtttgt aacttacacc tgctcccatg
     2221 ttgatatacc tgaaactcgt ggccagcaat gtgtcggctt gcaatgatct ccgttcgttt
     2281 gattcgccat cagcaaatat ttttaatgtg aggtcctggg tacttaatag tgtctttgac
     2341 aattcatatt tcggcctgag gaaccaggat ttttcctgat tgcccgaagt aagcaaatag
     2401 ctagattcaa gtatggaagt ccaagttttt tttacgtatc tattactaaa atcgtgtttg
     2461 ttggccttaa ataaattaga ttgtgaaaaa ctattgtacg tgtacttcag cgagaaggta
     2521 tttgattttg taagttgaat gccggcattg atccaccatt cgtccacatt tagtggcttg
     2581 tagttataat ctcggttaaa ttctgcattt ctgtacgggt taagtggcga aaaattcttg
     2641 ttcacgaatt cggtttctgc ataag
//
LOCUS       DAOQQY010000343         2662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_20697_length_2662_cov_5.454545, whole genome shotgun sequence.
ACCESSION   DAOQQY010000343 DAOQQY010000000
VERSION     DAOQQY010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20697_length_2662_cov_5.454545"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLE23_12145"
     CDS             complement(<1..246)
                     /locus_tag="PLE23_12145"
                     /inference="COORDINATES: protein motif:HMM:NF021882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMX84061.1"
                     /translation="MLYSFDDTIIAISTAPGVGAIAVIRLSGSRAIELADKYFKGKNL
                     QKAASHTLHFGQLMTKTDEILDEVVVGLFKAPNSYTRE"
     gene            695..2227
                     /gene="dnaB"
                     /locus_tag="PLE23_12150"
     CDS             695..2227
                     /gene="dnaB"
                     /locus_tag="PLE23_12150"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HMX84062.1"
                     /translation="MSNDSPFNPSGGFTKTRKSNKNTDLSELLYSKLTPQAVPFEEAV
                     LGAAMIDKDAFAVVLDILKEQSFYLNAHQHIYRAMLRLFDKLQPIDLLTVTEELRNEG
                     NLEKIGGPVYLVELTNKITSSANIEYHSRIIAQKHILRELIRTSNDVIKNAYDETIDV
                     FDLLDSAEKNLFEIAQHNMSRGSERVGSIASETLKKLESIPESATGTTGVPSGFIELD
                     KVTAGWQSSDLIIIAARPGMGKTAFTMALARNAAMDFNKPVAFFSLEMSNMQLVQRLI
                     SGEAEIEGSKLRTGRLEDSEWNLLHRTIERLADVPLFIDDTPGINIFELRAKCRRLKS
                     QHDIQMVIIDYLQLISGGTDNNKNGNREQEISGISRALKGMAKELNIPVIALSQLNRS
                     VEKSATSKRPQLSDLRESGAIEQDADIVSFIYRPEYYKITEDEEGNDTRGLAEIIIAK
                     HRNGALETVKLAFKSEFAKFADWTGNGFADVGYYNDFQSNAQSDSMRFNSRMNDDNED
                     PF"
     gene            2235..>2662
                     /locus_tag="PLE23_12155"
     CDS             2235..>2662
                     /locus_tag="PLE23_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HMX84063.1"
                     /translation="MQKKPIKSTPGFKKKAPRTNKPPQIEAFPEEMRLNRYVAQSGIC
                     SRRKAVDLVKEGKVSVNGEVILEPGFVVKPEDKVMYNGKVIKPDNNKIYILLNKPKNT
                     ITTVSDEKDRKTVMDIVGRTIKERIFPVGRLDRETTGLLL"
BASE COUNT          894 a          530 c          529 g          709 t
ORIGIN      
        1 ttctcttgta taagagttcg gtgctttaaa taatccaaca actacttcgt caagtatttc
       61 atctgtcttg gtcatcagtt ggccaaaatg caatgtgtgg gatgcagctt tctggaggtt
      121 tttacctttg aagtatttgt cagcaagttc gatggcacga gaaccgctaa gccgtatgac
      181 agcaatggca cctacacccg gagcggtaga aatggctata atcgtatcgt cgaagctata
      241 taacattatc tgtattttgg tttgtacaaa gctacaatta tttttttatt gcttgctgaa
      301 tcagatttat tcacatttat attatgagaa aatatttata tttataaaat aaaaatttaa
      361 acttaattca acaaattttc atccttttat atataatttt attcaatatg acttttataa
      421 atgtgcatac tatgcgtatt aaaaaaacgg aaaagcccta acgcaagatt atatcttacc
      481 aaataatttt cataaaaaaa caaatatttc gatttttatt aatactttaa ctgcgttaat
      541 gatgttatac taatattgag ttatcaacat gtctttagag ttactaacat tttttattgc
      601 attatcaaca ttggtgttac cggtatgtag ttttaattga ctagtttttt aaagtatatt
      661 tgtacacttt ttaacaacaa atttatcaaa cccaatgagt aacgattcac catttaatcc
      721 ttcgggtggt tttactaaga ccagaaagtc gaataagaat accgacctgt cggaattatt
      781 gtacagcaag ctcacaccac aggcagttcc tttcgaagag gcagtcctgg gtgcagcaat
      841 gattgacaaa gatgcattcg cggtggtact agacattcta aaagaacaaa gcttctatct
      901 gaatgctcac cagcacatct atagggcaat gttaagactg tttgataaac tgcagccaat
      961 agatttgcta acagttactg aagaactccg caatgaaggc aatctggaga aaatcggtgg
     1021 cccggtttat cttgtcgaac tgaccaataa gatcacttca tctgcgaaca tcgaatacca
     1081 ctccaggata attgcacaga aacatatact tcgggagctc atccgcacat cgaatgatgt
     1141 aattaaaaat gcctatgacg aaacaattga cgtgtttgat ttgcttgatt cggcagagaa
     1201 aaacctattc gaaatagcac aacacaatat gagccgtggc tcggagcgtg taggttcaat
     1261 agccagcgaa acgctaaaaa aactcgaatc gataccggag tctgcaacag gaactacagg
     1321 tgtaccgtca gggtttatag aactcgacaa ggtgaccgcc ggatggcaat cttctgacct
     1381 gatcatcata gcagcaagac ccggtatggg aaaaacggcc tttaccatgg cacttgcacg
     1441 caatgctgca atggatttta acaaaccggt tgcattcttt tctctagaaa tgtcaaatat
     1501 gcagctggta caacgtctga tatcgggtga agccgaaata gaaggaagta agttgcgtac
     1561 cggaaggctc gaggattcgg aatggaacct cttacaccgc accatagaac gactggctga
     1621 tgtgcctttg ttcattgatg atacgccggg catcaatatc tttgagctcc gtgccaaatg
     1681 ccgacgtctg aaatcgcagc acgatataca gatggtaatc atcgactacc ttcaactcat
     1741 ctccggtggt actgataaca acaaaaacgg caaccgcgaa caagaaatat ccggtatttc
     1801 aagggcatta aaaggaatgg caaaagaact gaacattccg gtaatagctt tgtcacagct
     1861 aaaccgttct gtcgaaaaaa gtgctacatc caaaagaccg cagctctcag acttgcgcga
     1921 atcgggtgcc atcgaacagg atgctgatat tgtttcattc atttatagac ccgaatacta
     1981 caaaataact gaagacgaag aaggtaacga cacacgtggg cttgcagaaa taatcattgc
     2041 aaaacacaga aacggagcgc tcgaaacagt aaagctagcg ttcaaaagtg aatttgcaaa
     2101 atttgccgac tggacaggaa atggcttcgc agatgttgga tattataatg acttccaatc
     2161 aaatgcacaa tcagactcta tgcgtttcaa ctcccgaatg aatgatgata atgaagatcc
     2221 tttttaattg aattatgcaa aagaaaccaa taaaatcaac accgggcttc aaaaagaaag
     2281 ctccaaggac taacaagcca ccacaaattg aagcattccc ggaggaaatg aggctcaatc
     2341 ggtatgtagc ccagtcgggt atttgctcca gacgcaaagc cgtggatctg gtaaaagaag
     2401 gtaaagttag tgtaaacggc gaagtcatcc tcgaacccgg attcgtagta aagccagagg
     2461 acaaggttat gtacaatggc aaggtcataa agcccgataa caacaaaatc tacattctac
     2521 tcaataagcc aaaaaatacc attacaactg tatctgacga aaaagacaga aaaactgtga
     2581 tggatatagt aggcagaacc atcaaggaaa gaatattccc ggtaggtaga cttgaccgcg
     2641 aaaccacagg cctgctgttg tt
//
LOCUS       DAOQQY010000344         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_21194_length_2624_cov_4.660958, whole genome shotgun sequence.
ACCESSION   DAOQQY010000344 DAOQQY010000000
VERSION     DAOQQY010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21194_length_2624_cov_4.660958"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLE23_12160"
     CDS             complement(<1..220)
                     /locus_tag="PLE23_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HMX84064.1"
                     /translation="MYILGINAYHADSSAAIFKDGIMIAATEEERFRRVKHWAGFPAL
                     AIEFCLKEAGITIDELDHIAIGRDPKAIF"
     gene            complement(338..1078)
                     /locus_tag="PLE23_12165"
     CDS             complement(338..1078)
                     /locus_tag="PLE23_12165"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HMX84065.1"
                     /translation="MFYLEKFVRMLIELPVLLTICFILAALLRYRNSKAAWPLTATLL
                     LLVWILSTKPGVILMTAPLVAGLHEADTGTLPVIADIVVLGGGCRRQSGHSTAEKLSD
                     PSLRRTLEGYRIWSRLPGSTLILTGIDWQRHCNTAGIAKQLISSWSADTTRIRLLNPV
                     FNTRQEARAYVSSNGTGRPVILVTSAIHMPRALRNFRRQNITVTAAPTDFPVSDTRIR
                     LSDLIPQSNAFSTSSAAWIEWIALLTGK"
     gene            complement(1091..2233)
                     /locus_tag="PLE23_12170"
     CDS             complement(1091..2233)
                     /locus_tag="PLE23_12170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX84066.1"
                     /translation="MQKLLILTDWFTPAYKAGGPVRSIENLARLLKDDFEIFILTGDR
                     DLGDRHPFEGITPDAWILSDHNHHVRYVSPGKQNRHHIKKILLDIAPDILYLNSMYSR
                     SFTLLPLSLLSSLPPDIRTILCPRGMLKDSALQYKSLKKKLFLYLMKFLHLGRRISFH
                     ATDSQEAADIRKWFGRQAKLTIVPNVPALLDYQRCSKQENQLRVLFVGRIHPVKNLKF
                     AIETLQHTNAKLHFEIIGNIEDEKYWQECSEVINQLPSNVTVKFSGALPHKSIIRALT
                     DCHLFYLPTQGENFGHAIFEALSVGRPVLISDQTPWRTLKEQNAGHDITLNNKTEFAN
                     YVGEVAAMHQAAFDLSCQAAHNLAKHYISSHHFLQKYRQLFGCTTD"
     gene            complement(2284..>2624)
                     /locus_tag="PLE23_12175"
     CDS             complement(2284..>2624)
                     /locus_tag="PLE23_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HMX84067.1"
                     /translation="GVPDQYKYPTKPKKLLVDALTPLIPDEIVHRPKMGFTFPWELWM
                     RNELISFCDARINRLVQRDLFNKEYVLSLWKGFLAGKNVNWVKVWLLVVLEHWLDRNN
                     IELVSSKLQV"
BASE COUNT          573 a          537 c          769 g          745 t
ORIGIN      
        1 aaaatatagc cttagggtca cgacctatgg caatgtggtc gagttcatca atggtgatgc
       61 cggcctcctt cagacagaac tcaatagcta atgcaggaaa tcctgcccag tgctttacac
      121 gtctaaaacg ttcttcttca gtagctgcaa tcatgatgcc atccttaaat atagcggcgg
      181 acgagtctgc gtggtatgcg tttatgccta agatgtacat tgtggtatgt ttttaatgct
      241 tggttttgat atattgttta tgtaagattt tttgggcgtg cccctgtcgg gtcgggctaa
      301 cattcaaata tctgcttatc aagtttgttg tattcatcta tttgccggta agcagggcaa
      361 tccattcgat ccaggcggcg gatgaggtgc tgaatgcgtt ggattggggt atgaggtcgc
      421 tgagcctgat gcgtgtatcg ctgaccggaa aatcggttgg ggcggcagta acggtgatgt
      481 tttggcggcg aaaattgcgc aaggctcgcg gcatgtgtat ggcggatgtg acgaggatga
      541 cggggcgtcc ggtgccgtta ctgctgacgt aagcgcgggc ttcctggcgt gtgttgaaga
      601 ccggattgag caggcgtatg cgtgtagtgt cggctgacca gctgctgatg agctgtttgg
      661 ctatgccggc ggtgttgcag tgacgctgcc agtcgatacc ggtgagtatg agtgtgctgc
      721 cgggcaagcg gctccagatg cgatagcctt cgagtgtgcg gcgcagggac ggatcggaga
      781 gtttttcggc agtcgaatga cctgattggc gcctgcagcc tccgcctaag actacgatgt
      841 cggctatgac agggagtgta cctgtgtctg cctcatgcag gccggctacg agcggtgcgg
      901 tcatgagtat aacgccgggt ttggtactca ggatccagac gagcaggagc aatgtagcag
      961 taagtggcca ggctgccttg ctgttgcggt agcggagtaa ggctgcgagt atgaagcata
     1021 tagtgagcaa caccggcaac tcgatgagca tcctgacgaa tttttcgagg tagaacatgc
     1081 gttatccggg ttagtcggta gtacagccga atagctggcg gtatttttgt aggaaatggt
     1141 gagaactgat atagtgtttg gctaggttgt gggctgcctg acagctaagg tcgaaagcgg
     1201 cttgatgcat ggcagctact tcgccaacat agtttgcaaa ttctgttttg ttgttcaagg
     1261 tgatatcgtg acctgcattt tgctccttga gtgtgcgcca gggtgtctgg tcgctgatga
     1321 gtacagggcg gcctactgag agtgcttcga agatggcgtg accgaaattc tctccttgcg
     1381 tgggcaggta gaacagatga caatccgtca gtgcccggat gatgcttttg tgtggcaatg
     1441 ctcctgaaaa cttgactgtg acgttggagg gcagctggtt gataacttcg ctacattcct
     1501 gccagtattt ttcgtcttcg atattaccga ttatttcgaa atgcagcttt gcattggtgt
     1561 gctgaagggt ttcgattgcg aatttcaggt ttttgacagg gtggatgcgc cctacgaaca
     1621 acacccgcag ttggttttct tgcttgctac agcgctggta gtccagaagt gccggcacat
     1681 ttggcactat tgtcaatttt gcttgccgac cgaaccattt tcttatatcc gctgcttcct
     1741 gcgagtcggt agcgtggaag ctgatacgcc tgccaagatg taggaatttc atcaggtaca
     1801 ggaagagctt ctttttgagt gacttgtact gcaacgcact gtctttgagc ataccgcgtg
     1861 gacataggat ggtacgtatg tcgggaggca ggctgctgag caggctgagt ggcagtaatg
     1921 taaaactacg tgaatacata gagttgaggt agagtatgtc gggtgctatg tcgagtaata
     1981 tttttttaat gtggtgtcgg ttttgtttgc cgggcgatac atatcttaca tggtgattgt
     2041 ggtctgacag aatccatgca tcgggtgtga taccttcgaa cggatggcgg tcgccgagat
     2101 cccgatctcc tgtgagtatg aagatttcga agtcgtcttt aagcagccgg gcgagatttt
     2161 cgatactgcg cacagggcct cctgccttgt atgcaggtgt aaaccagtct gtgagtatga
     2221 ggagtttttg catggggagt cagaaggatg aactttctgt cacgcttttt aatgctttaa
     2281 aaatcaaacc tgtagtttac tgctaactag ttcgatatta ttacggtcaa gccaatgttc
     2341 taacactacg agcagccata ctttaaccca gttaacattt ttgcccgcca ggaatccttt
     2401 ccacaagctt aacacgtatt ctttattgaa caaatcgcgc tgtacaagtc tgttgatacg
     2461 ggcgtcgcag aaggatatga gttcgttgcg catccagagc tcccaaggga atgtgaagcc
     2521 cattttgggt ctgtgtacaa tctcatcggg tataaggggt gtgagtgcgt caaccagtaa
     2581 ttttttgggt ttagttgggt atttgtactg atcgggtacg ccga
//
LOCUS       DAOQQY010000345         2624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_21197_length_2624_cov_4.045543, whole genome shotgun sequence.
ACCESSION   DAOQQY010000345 DAOQQY010000000
VERSION     DAOQQY010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21197_length_2624_cov_4.045543"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..2438
                     /locus_tag="PLE23_12180"
     CDS             156..2438
                     /locus_tag="PLE23_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX84068.1"
                     /translation="MKFLKYTMAVIFALTVNHLFAQTNGGVRGTILDKATGNPVAYAV
                     VQLVGTQYGATSNETGFYSINNVPVGEYVMLVKLFGYDSLSMDVSIKRGGYVNKQILL
                     SESGINLKEFEISAEREAAKTEVLVSKVTVTPKQIRALPSTGGEADIAQYLPVLPGVI
                     STGDQGGQLYIRGGAPIQNKVLIDGLTIYNPFHSIGFFSVFETEAIRSVDVYTGGFGA
                     QYGGRISAIVDIKTKDGNKKRLSGLISGSPFQSKVLLEGPISVLKESGGGSTSFILTG
                     KTSYIDQTSKQLYKRVNNGNGLPFSYTDLYGKVSFNAANGSKVNLFGFNFLDKVKYQG
                     IADLGWKATGGGASLSLIPGSASMLIGANLGYSDYAIDLKEADGAPRQSKINGFNLGL
                     DFTSFGAHDEFRYGFDINNFNTDFAFTNFKKLNIKQEQFTTELSGFFSYKYKWPKLVI
                     EPGLRVQYYASLSESSLEPRLGLKWNATDKLRFKLAGGMFSQNLLSTVSEQDIVNLFV
                     GFLSGPEEQLLTKDEKSPADSKLQKAVHLITGVEYDVNKNLTINLEPYVKKFTQLINL
                     NRNKTKFSDPNYESETGDAYGIDLSAKYDRRNFYAWVTYSLGFVNRDDGTQIYPTNFD
                     RRHNVNLVATYNFGKDKTWEFGARWNFGTGFPFTRTQGFYEFFQFQDGVSTDVNSGNG
                     DLGIIYSSKRNDGRLPTYHRLDLSLKKTIIFSKSSKLEINATATNAYDRANIFYFDRV
                     RYSRVDQLPILPSIGLIFHF"
BASE COUNT          778 a          533 c          550 g          763 t
ORIGIN      
        1 gtaaaatttt tttggaagaa atattagatt cgggtggttt ggttaaaatg tattaatctg
       61 cctcccgaat ttttattttg gaaaagctta aaaaatttac atttattcgg attagctcgt
      121 atttttgtct gttcttaaaa ttttacaaat cgcttatgaa gttcttaaaa tacacaatgg
      181 cagtgatttt tgcattaact gtcaatcact tgtttgcaca gacaaatggt ggagtacgtg
      241 gtaccattct cgataaagca acgggtaatc cggttgcata cgccgtggtt cagttggtcg
      301 gtacgcaata tggggcaacc tccaacgaga ccggatttta ctcaattaac aatgtgccgg
      361 tcggtgaata tgtgatgtta gtcaagctct ttgggtatga cagcctgtca atggatgtat
      421 caatcaaacg cggtgggtat gtcaataaac agatactcct ttctgaaagt ggtatcaacc
      481 taaaagagtt tgaaatcagt gccgaacgtg aagcagcaaa gaccgaagtg cttgtatcga
      541 aagtaacggt tacaccaaaa caaatacgcg cattaccttc taccggtggt gaagctgata
      601 tagcacagta tcttccggta ctgcccgggg ttatctctac cggcgaccaa ggtgggcagc
      661 tgtacatacg tggtggtgcc cctatacaaa acaaagtact catcgacggt cttaccattt
      721 acaatccatt ccattccata ggtttctttt cggtattcga aacagaagcc atccgttcgg
      781 tagatgttta tactggagga tttggtgcgc agtacggagg cagaatttca gctattgttg
      841 atatcaagac caaggacggc aacaaaaaac gacttagcgg tttaatatct ggtagcccat
      901 tccaaagtaa ggtgttgctc gaagggccga tttcggtact gaaagaatcg ggaggaggta
      961 gtacttcatt tattctgacg ggaaaaactt cttacattga ccaaacatcg aaacaactgt
     1021 ataaacgtgt gaacaatggt aatggtttgc ctttttctta taccgatttg tatggcaaag
     1081 tatcattcaa tgctgccaac ggaagtaagg tcaacctttt tggattcaat tttcttgaca
     1141 aggtcaaata tcaaggaatc gcagatctcg gctggaaggc aaccggtggt ggtgcgagtc
     1201 ttagcctcat acccggctct gcttcaatgc tcataggagc taacttgggt tattctgatt
     1261 atgcaattga cctgaaagaa gcagacggtg ccccaagaca aagtaaaata aatggcttta
     1321 atcttggtct tgactttact agcttcggcg cacatgacga attcaggtac ggatttgata
     1381 tcaataattt taatacagat tttgctttta ctaattttaa aaagctgaat atcaaacaag
     1441 aacagtttac gactgagctg tccggtttct tttcgtataa atacaaatgg cccaaactcg
     1501 taattgagcc aggtctgcga gtgcagtatt atgcttcatt gagcgaatca agtctcgaac
     1561 cacgtcttgg gctgaaatgg aacgccaccg ataagcttcg attcaaactt gcaggtggga
     1621 tgtttagtca gaatctgctt agcacagtgt ctgagcagga tattgtcaat cttttcgttg
     1681 gtttcctttc cgggccggaa gaacaattac ttaccaagga cgaaaaatct ccagcagata
     1741 gtaagcttca aaaggctgta cacctaataa cgggtgtcga atatgatgta aataaaaatc
     1801 tgaccatcaa cctggagcct tacgttaaga aatttaccca actcataaac ctgaacagga
     1861 ataaaaccaa attctctgac ccgaactacg aatctgaaac aggtgatgct tatggtattg
     1921 acctttctgc caagtacgat aggcgcaatt tttatgcatg ggtcacgtac tcactcgggt
     1981 tcgtaaacag agatgacggc acacagattt accctaccaa ttttgacaga agacacaatg
     2041 tcaatctcgt agcaacatac aattttggta aagataagac atgggaattc ggcgcacgtt
     2101 ggaattttgg taccggtttt ccgtttaccc gcactcaagg attttatgaa tttttccaat
     2161 tccaggatgg agtttctact gacgttaact caggtaatgg cgaccttggt atcatttaca
     2221 gttcaaaaag gaatgatggc cgcctgccta cctatcacag actcgatctt tctttgaaga
     2281 aaaccatcat attttcaaaa tcatccaagc ttgaaatcaa cgctaccgct acaaatgcct
     2341 atgatagagc gaacattttc tacttcgacc gcgttagata ttcaagagtg gatcagttac
     2401 cgatacttcc tagtattggt ctgatttttc atttttgatg accggatttt atcaacatca
     2461 taaaactgcc tcctatgaaa catctgctta cactcatcct gattgtgact ggaattctcg
     2521 tttcagcaca aaatatcctt ccggcaggtt ttgccaaagg cgagaaagaa ctgatgcctc
     2581 tttatctcga aaatcttgaa gcaaatggga taactactcc gcct
//
LOCUS       DAOQQY010000346         2618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_21278_length_2618_cov_4.717519, whole genome shotgun sequence.
ACCESSION   DAOQQY010000346 DAOQQY010000000
VERSION     DAOQQY010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21278_length_2618_cov_4.717519"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..520)
                     /locus_tag="PLE23_12185"
     CDS             complement(38..520)
                     /locus_tag="PLE23_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HMX84069.1"
                     /translation="MLSGLRSFFSAPVNLLLASILVLILSMACRHSAQTDRHTRQHHT
                     DQTAKQAIPTENKSSELKKSASRSPKIPPEAYDVAAYALAHQGETMPGYKGNTPFRNR
                     EKILPIKNAQGKKLSYKEYDIYPLQQGKRRGARRVVIDSDGNAYYTKDHYRTFQIMQK
                     "
     gene            612..1550
                     /locus_tag="PLE23_12190"
     CDS             612..1550
                     /locus_tag="PLE23_12190"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMX84070.1"
                     /translation="MKIKFLLSLFFGLGVAMLASAQTPQAFHEDTVAINGTQLYVKTV
                     GQGEPLLLINGGPGLSHDYLLPHVLPLADKHMLILYDQRASGRSSIILDSAGMRLHNF
                     VEDIEGLRKHFGIKKLNIVGHSWGGLLVSLYAARYPKRVKSVVLWDCMPLSAHFRDVM
                     LENQKSSLSDEERANQVKIARSNEFKSGDPNELKRFYRSVFKSSVFIQKKVYKLDFYF
                     NEAYRQQQYLIGFLSPDLKDYDFFPLLKKIKVPVLIFHGSYDPMPVENAEWMHEKLKK
                     SELVILQECGHFPFLEKPDELFAKMEQFYAKLNAKK"
     gene            complement(1553..>2618)
                     /locus_tag="PLE23_12195"
     CDS             complement(1553..>2618)
                     /locus_tag="PLE23_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMX84071.1"
                     /translation="YYGADFRRNPVGTGAFLFKRWIENQTLFLNRNPHYFESVNGQRL
                     PYLEGVRVDFISDRKTAFLKFMKGNIDFFSGIESSFANALLTPDGNLQKNIQDKINFI
                     KNPYLNTEFIGINYTILPDNHPLRNKYFRQALNYSINRRQMLQLLRNNIGKPAESGIL
                     PHGLPNYNDDTPGRYSYDISKAAELLTKAGYPNGKGLPQLTLHCNKDYLDICTFLSRQ
                     WQQAGIPVTIEPVESATLREMMRNGKTAFFRASWIGDYPDEENYFSIFYSKNDAPPNY
                     TRFNNKSYDQLYTQLLTLTDERQIRNTCNRMNQIILEEAPVVLLFYDETAQFVQKNIS
                     GLRSDIMNILSVKKIRKTTR"
BASE COUNT          639 a          453 c          704 g          822 t
ORIGIN      
        1 gtaatctcct tgagtgaatg gtatatgccg ctttggttca tttttgcatg atttggaagg
       61 ttctgtaatg gtcttttgtg taataggcat tgccgtcaga atcaatgact acacgtctgg
      121 cgccccgtct tttaccttgc tgtagcggat agatgtcgta ttctttgtaa ctgagttttt
      181 tgccttgtgc atttttgatg ggtagtattt tttcgcggtt tctgaatggt gtgttgcctt
      241 tgtagccggg catggtttcg ccttggtgag cgagggcgta ggcagccaca tcgtatgctt
      301 caggaggtat ttttgggcta cgagatgcag attttttaag ttctgagctt ttgttttcgg
      361 ttggtatggc ttgcttggca gtttggtcgg tgtggtgttg ccgggtatga cggtcggtct
      421 gtgcagagtg ccggcaagcc atactgagta tgagtaccag tatgcttgcg agtagtaagt
      481 taacaggagc agaaaagaat gaccggagac ctgacaacat ggattgtgtt attatttgtt
      541 taaatattgt aaaacatttt ggactgtaaa gatatctata ttatcattac attagtattc
      601 aaatgcacgg aatgaaaata aagttccttt tgtcgttgtt ttttggtctt ggagtggcaa
      661 tgttggcttc tgcacagacg cctcaagcat tccatgaaga tacagttgcc atcaatggaa
      721 cacaacttta cgtaaagacg gtggggcagg gcgagccatt gttgttgata aatggaggcc
      781 cggggcttag tcacgattat ttgttgccac atgtactacc gctggccgac aaacacatgc
      841 tcatcctgta cgaccaacgg gcatcgggca ggtcgagcat catattggac agtgcaggca
      901 tgaggctgca taattttgta gaggatatcg aaggactgcg caagcatttc ggtatcaaaa
      961 aactcaatat agtaggccat tcgtggggag gtctgctggt gagtttgtat gctgccaggt
     1021 atccgaaacg ggtgaagtcg gtcgtgttgt gggactgtat gccattgtct gcacattttc
     1081 gcgatgtgat gctcgaaaac cagaaaagta gcctgtcgga tgaagaacgt gccaatcagg
     1141 taaagatagc gcgttcgaat gaattcaaga gtggtgatcc aaacgagtta aagcgttttt
     1201 acaggtcggt gttcaagtcg tcggtgttta tacagaagaa ggtatataag ctcgattttt
     1261 actttaatga agcttaccgg caacagcagt atctgattgg ttttttgtct cctgacctga
     1321 aggactatga tttctttcct ttattgaaga aaattaaagt gccggtactt atatttcacg
     1381 gtagttatga tccgatgcct gtagagaatg ctgagtggat gcacgaaaaa ctgaaaaaat
     1441 cggagcttgt aatattgcaa gaatgtggtc attttccttt tcttgaaaaa cccgatgagt
     1501 tgtttgcgaa gatggagcaa ttctatgcaa agttgaatgc caagaagtga tgtcagcggg
     1561 ttgtttttct tatctttttg acagagagta tgttcataat gtcggaccgg aggccagata
     1621 tgtttttttg aacaaactgt gctgtctcat catagaaaag caggactacc ggagcttctt
     1681 cgagtataat ctggttcata cggttacaag tattgcgtat ttgcctctca tcggtaagtg
     1741 tgagtaactg tgtatagagt tggtcgtatg atttgttgtt aaatcgggtg tagttgggcg
     1801 gtgcgtcgtt ttttgagtaa aaaatactaa aataattttc ttcatcgggg tagtcaccta
     1861 tccatgaagc acggaagaaa gcggttttgc cgttgcgcat catttcgcgg agtgttgccg
     1921 actctaccgg ctcaattgtt accggtatgc ctgcctgctg ccactgtctg ctgagaaatg
     1981 tgcagatatc gaggtagtct ttgttgcagt gcagcgtgag ttgtggcaag ccttttccat
     2041 tcgggtagcc tgctttagtg agcagttcgg cggctttact tatatcgtag ctgtatcggc
     2101 cgggcgtgtc atcattgtag tttggcaagc catgaggaag gatgccggat tcagcaggtt
     2161 tgcctatgtt attgcgaaga agttggagca tctgacggcg attgatactg tagttgagcg
     2221 cctggcggaa atatttgttg cgcaggggat gattgtcggg cagaatggta taattaatgc
     2281 cgatgaattc ggtattcagg taagggtttt tgataaagtt tattttgtct tgaatatttt
     2341 tttgtaaatt tccgtccgga gtgagcaagg cgttggcaaa ggaagattcg atgccggaga
     2401 aaaaatcgat atttcctttc atgaatttga gaaatgctgt tttgcggtca gatatgaaat
     2461 cgacccttac accttcgaga tagggaagtc tttggccgtt taccgattcg aaataatgcg
     2521 gattgcggtt taggaacagt gtttggtttt cgatccatcg tttgaaaagg aaggcaccag
     2581 taccaaccgg attgcgtcgg aagtcggccc cataatag
//
LOCUS       DAOQQY010000347         2609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_21412_length_2609_cov_3.467894, whole genome shotgun sequence.
ACCESSION   DAOQQY010000347 DAOQQY010000000
VERSION     DAOQQY010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21412_length_2609_cov_3.467894"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="PLE23_12200"
     CDS             complement(<1..315)
                     /locus_tag="PLE23_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84072.1"
                     /translation="MKKILQSALLLVGFIYNISAQSCCPEITKSSVNTGNCIVTNGGS
                     CGMCKDATVSLSLDDGKNLPVGGQLSWYYGTSPTFNPAIGQGTLIGTVSIPSSDCDNA
                     SGV"
     gene            705..1010
                     /locus_tag="PLE23_12205"
     CDS             705..1010
                     /locus_tag="PLE23_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84073.1"
                     /translation="MYAILISLVIGLFVAMLFLNVYFRVKVLKVYKVLVQNRVEFGAK
                     HLFNTRLMEEEVVPKYPKQAEEIRTFVRHMKYSINMASVLIGLITVFGAILMYYRKG"
     gene            1074..2075
                     /locus_tag="PLE23_12210"
     CDS             1074..2075
                     /locus_tag="PLE23_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMX84074.1"
                     /translation="MVKIGVAGAGHLGKIHIKCIQSAQGLSLVGFYDTDAGVREKVAA
                     EFGIKAFDDYDEMLRHCDAVDIVVPTIFHHKIAKKAIEAGKHIFIEKPITHTLAEAEE
                     LLDIAKQKKLKIQVGHVERFNPAFLAISHLALNPMFIEAHRLAAFNPRGTDVSVVLDL
                     MIHDIDIILACVKSEVKEIRASGVSIISNTPDISNVRLEFENGAVANLTASRISMKQM
                     RKLRIFQPDAYISLDFLEKSSQIIRLYNEEPEGREGTMLSLDTPAGSRFIEIEMPESA
                     PVNAIQMELEKFCEAIVNDTQPIVSGEQGYHALKLALKVLDEIEEHTLRATKMNTNA"
     gene            2068..>2609
                     /locus_tag="PLE23_12215"
     CDS             2068..>2609
                     /locus_tag="PLE23_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84075.1"
                     /translation="MLRIYFVLLIIVGLFGCNSFDQPDPVASYVHIPSMSMKAKPGYG
                     SVQQDFPLAFVYLDGTLMGAFSIPATIPLIKAGDSEITVFAGIIKNGIKETAEAYPLV
                     DRYVKNIKLTPGKVDTIWPVFSYRDDVVPQIVEDFESSAHLFPTILNGYGIGFTANEA
                     FEGFKSGIMLMDTIKPLNEVG"
BASE COUNT          804 a          510 c          572 g          723 t
ORIGIN      
        1 gacaccactt gcgttgtcac agtcagatga aggaatactt actgtgccta tcagagtacc
       61 ttgaccaatg gcagggttaa atgttgggct ggttccgtaa taccaggaca gctgaccacc
      121 taccggtaga tttttgccat catctaacga caaacttact gttgcatctt tgcacattcc
      181 acatgagcca ccattggtga caatacaatt tccggtattt acagaacttt tggtaatttc
      241 cgggcaacat gactgtgcag atatattgta gataaaaccc acaagcaaaa gggcagactg
      301 tagaattttc ttcattggca gttaaacgtt ttttcggatt cccattattt tcagcataaa
      361 actaagtaaa tttaatttac atttcaacaa aaaaagtaaa aatacaggat gcaggaaata
      421 aatttgggag gatcagtaga atcagccaga atgaaattca ccctatgaat catctgattt
      481 tttggtatta tatcaattac agaaggctgg tatatttatg gatataaagt ataagttagc
      541 ggttggcctc tcatcagcga atgttacctt gaggtttaat tctttactgc aagcaatcat
      601 caacaatatt gatatgaata tgcaaggcgc cggaagagtg gtaaaaaacc aatttcactc
      661 aattcactaa taatgcttac ttttgggcag caaatgattg tcttatgtat gcaatactta
      721 tatctctggt catcggattg tttgtagcta tgctcttcct gaatgtgtat ttcagagtaa
      781 aagtgctgaa ggtgtataaa gtgctggtac aaaacagggt agagtttggg gcaaaacatt
      841 tgttcaatac ccgtttgatg gaagaagaag tggtgcccaa atatccgaaa caggctgaag
      901 aaattcgcac gtttgtgcgt cacatgaaat attccatcaa tatggcatcg gtgcttatag
      961 gcctcattac ggtgttcggg gcaatactga tgtattacag aaaaggatga ttcaggcatt
     1021 tcggttttga taataggcag ctatttcatt agctttgctg ttaataataa tatatggtta
     1081 agataggcgt agcaggtgcc ggacatctgg gcaaaattca cataaaatgc atccaaagtg
     1141 cacaaggatt gtcactggtg ggattttatg acacggatgc cggtgtaaga gaaaaggtag
     1201 cagccgagtt cggtataaaa gcatttgacg attatgatga aatgcttaga cattgtgatg
     1261 cagttgatat tgtggttcct actatttttc atcataaaat agcaaaaaag gccattgaag
     1321 ccggcaaaca tatttttatt gaaaaaccaa taacacatac attagctgag gcagaagaac
     1381 tgcttgacat agcaaaacaa aaaaagttga aaatacaggt tgggcatgtc gaacgattca
     1441 atcctgcttt tcttgccatc agccacctgg cactcaaccc gatgtttatt gaagcacacc
     1501 gcctggcagc cttcaacccg agaggtactg atgtgtcggt cgtactagac ttgatgatac
     1561 acgatataga cattatactt gcatgtgtga agtcggaagt aaaggaaatt cgggcttccg
     1621 gtgtttcaat catcagcaat acacccgaca taagcaacgt acggctggag tttgaaaatg
     1681 gtgccgtcgc caaccttact gccagccgaa tatcaatgaa gcagatgcgc aaactccgga
     1741 tatttcaacc tgacgcctat atcagcctcg attttcttga gaaaagttca cagataatca
     1801 ggttgtataa tgaggaacct gaaggaagag aaggtaccat gctgtcactt gacacacccg
     1861 caggctcacg atttattgaa atagaaatgc ctgaatcggc accggtaaat gccatacaga
     1921 tggagctaga aaaattttgc gaagccattg tcaatgatac acagccaatt gtatccggtg
     1981 agcagggcta ccatgcgtta aaactggcat taaaagtatt ggatgaaata gaagaacaca
     2041 cattgcgggc aacaaaaatg aatacaaatg cttagaatat attttgtttt actgattatt
     2101 gtcgggttat tcgggtgtaa cagttttgac cagcccgacc cagtggcatc ctacgtgcat
     2161 ataccttcga tgtctatgaa ggccaaaccg ggttacggtt ctgtacagca ggatttcccg
     2221 ttggcatttg tatatcttga cggcacattg atgggtgcgt tctctattcc tgcgaccatt
     2281 ccactcatca aagccggtga ttcggagatt actgtttttg caggtattat aaagaacggg
     2341 atcaaagaaa ccgccgaagc ctatccgctt gttgacaggt atgtcaagaa catcaagctc
     2401 acgcccggca aagtcgatac catctggccg gttttcagct accgcgatga tgttgtaccg
     2461 cagatagtag aagactttga atcatcggca catctttttc cgaccatact caacggctac
     2521 ggaatcggat ttacggccaa tgaagctttt gagggtttta aaagcggcat tatgctcatg
     2581 gataccatca aaccactcaa cgaggtggg
//
LOCUS       DAOQQY010000348         2580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_21778_length_2580_cov_3.507723, whole genome shotgun sequence.
ACCESSION   DAOQQY010000348 DAOQQY010000000
VERSION     DAOQQY010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21778_length_2580_cov_3.507723"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="PLE23_12220"
     CDS             complement(<1..504)
                     /locus_tag="PLE23_12220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMX84076.1"
                     /translation="MVKQLSVLSLLILLLLISCRNDKDNAIALKVFKYNQPNNVTSLD
                     PAFARSQTNIWAVDHLYNGLVQLDDSLRVVPCIAKSWHISDDGRTYEFLLRNDVRFHK
                     NKCFGEIGTRQVTAEDFEYSLRRLLDPEVQSPGSWVFKGKVDSIRPFEAINDSIFRIY
                     LTSPFKPM"
     gene            complement(515..1057)
                     /locus_tag="PLE23_12225"
     CDS             complement(515..1057)
                     /locus_tag="PLE23_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMX84077.1"
                     /translation="MISVFSASNRADNKTFPFASAYYNILRELTNDKVQLLDFRQLPS
                     EFLHDMMYDSENQSPIIKDIHNQYLASTNKWVFFIPEYNGSFPGILKLFIDACSVNNL
                     KDSFYGKKAAFVGIADGRSGNVKGMDHFTSVMNHIGTVVYPYKLPVSQISKLMDAELN
                     ITDQMTLSLFERHAKAFLEF"
     gene            1163..1858
                     /locus_tag="PLE23_12230"
     CDS             1163..1858
                     /locus_tag="PLE23_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMX84078.1"
                     /translation="MKLVHYGHSAFQILTAGKSILIDPFFSGNELSKGIMDAETIEAD
                     YILLSHAHADHSYDVERIAKRTGAKVVGVYELAEHYKALGIESHGMNIGGKFEFDFGV
                     VKMVNAVHSSSFSDGKYGGVAGGFVVWNDEVCFYFAADTALTLDMQLIPLTCPPLDFA
                     ILPVGDNYTMGYEDAIIASDFLQCDRIVGSHYDTFPVIKMDHTKAVEAFKNENKQLIL
                     LAPGKSIEINHES"
     gene            1848..>2580
                     /locus_tag="PLE23_12235"
     CDS             1848..>2580
                     /locus_tag="PLE23_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMX84079.1"
                     /translation="MNRKGKIVAIANQKGGVGKTTTSINLAASLAIMEKKVLLIDADP
                     QANATSGTGLTIHDDEVTLYECLVDGADPRLAIKQTETPNLDVLPSSIDLVGAELEMA
                     EMTKREFILKGILEQVRYNYDYVIIDCLPSLGIITVNALTAADSVLVPVQCEIFAFEG
                     LSKLKNTINMVRQYLNPALQMEGIVLSMYDKRLRLANIVMNEVKRHSSDRVFDTIIHR
                     NARIGEAPSMHQPVILYDATCKGASS"
BASE COUNT          764 a          481 c          608 g          727 t
ORIGIN      
        1 cataggtttg aagggcgatg taaggtagat gcggaaaata ctgtcattaa tagcttcaaa
       61 tggcctgata ctgtccactt tacctttgaa tacccatgaa cccggggact gcacttctgg
      121 gtcaagcagt cttcgaagac tgtattcgaa gtcctctgca gtgacttgtc gtgtgccgat
      181 ttcgccaaag catttgtttt tgtgaaaccg gacatcattg cgcaggagga attcgtatgt
      241 gcggccgtca tccgatatat gccatgattt ggcaatacaa ggaaccaccc taaggctgtc
      301 gtcaagctgt acgagtccgt tgtataaatg atctacagcc cagatgttgg tctggcttct
      361 ggcaaatgcc ggatcgagag atgtaacatt gttgggttgg ttgtatttga atactttgag
      421 tgctattgca ttgtctttat cattgcgaca tgaaatgagc aataacaaga tcaaaagtga
      481 aagtacagat aattgtttca ccatcaaaaa cgggttaaaa ttcgaggaaa gctttagcgt
      541 gtcgttcgaa gagtgagaga gtcatttggt cggtaatatt cagttcggca tccatgagct
      601 tgcttatctg cgaaaccggt aatttgtatg ggtaaacgac cgtaccaatg tggttcatga
      661 cgcttgtgaa gtgatccatg cctttcacat tacccgacct gccatcagcg atgcctacga
      721 atgcggcttt tttgccatag aatgagtctt tgagattatt gacagaacag gcatcaataa
      781 acagtttgag gataccagga aacgaaccgt tgtattcagg aatgaagaaa acccatttgt
      841 tggttgaagc cagatattga ttgtggatat ccttgatgat gggcgactgg ttttcggagt
      901 catacatcat atcgtgaagg aattcggaag gcaattgccg gaaatcaagc agttgcacct
      961 tgtcgttcgt aagttctcga agtatgttgt aataagctga agcaaatgga aatgttttgt
     1021 tgtcggctct gtttgatgct gaaaatactg aaatcattct gtctctattg tctggcttta
     1081 tgttatttaa atgtaaaagt acgtagttag atggttaata tctgcaaata gcgtaatttt
     1141 gcagactgaa aaataaattt acatgaaatt agtacattac ggacattcag cctttcagat
     1201 attgactgcc ggaaaaagca tactcatcga tccgtttttt tcgggcaatg agctttcaaa
     1261 aggtataatg gatgctgaaa caatagaagc tgactacatt ttattgtcgc atgcgcatgc
     1321 cgaccacagt tatgatgtag agagaatagc caagcgaacc ggtgctaagg tagttggtgt
     1381 ttatgagttg gctgagcatt acaaggcatt aggcatcgaa tcgcatggaa tgaatatagg
     1441 agggaagttt gaatttgatt ttggtgttgt aaaaatggtc aatgcagtac attccagtag
     1501 tttttctgac ggaaaatatg gtggagtagc aggtggtttt gtagtttgga atgacgaggt
     1561 gtgtttttac ttcgcagctg atacggcact tacgctggat atgcaactga taccgttgac
     1621 gtgtccgcca cttgattttg caatattgcc ggtaggcgac aactatacaa tgggttatga
     1681 agatgccatt atagcaagtg attttctgca atgcgaccgc attgtaggca gccattatga
     1741 tacctttccg gtaataaaaa tggatcatac aaaagccgta gaagcattta aaaatgaaaa
     1801 caagcaacta atattattag cacccggaaa atccatagaa attaatcatg aatcgtaaag
     1861 gaaaaatagt agccattgcc aaccagaagg gaggtgtagg caaaacaacg acaagcatca
     1921 atctggctgc aagccttgcc attatggaaa agaaggtttt gctcatagat gcagaccctc
     1981 aggcaaatgc tacctccggc acaggactaa ccattcatga tgatgaagta acgctgtacg
     2041 aatgtctggt agatggggca gaccctcgcc tggcaatcaa gcagaccgaa acacccaatc
     2101 tggatgtatt accttcttcc atagatcttg taggtgctga gctcgaaatg gcagaaatga
     2161 ccaaacgcga atttatactg aaaggaatac ttgaacaggt aaggtataac tatgattatg
     2221 ttatcattga ctgccttcct tcactcggta tcataactgt caatgcactt acagctgccg
     2281 attcggttct cgtaccggta cagtgcgaga tatttgcatt cgaagggctt agcaaactga
     2341 agaataccat caatatggta agacagtatc ttaaccctgc attacagatg gaaggtatcg
     2401 ttctttcgat gtatgacaag cgactgcgcc tcgctaatat tgtgatgaat gaggtaaaac
     2461 gccacagttc ggatcgtgta tttgacacca tcattcaccg caatgcaagg attggcgaag
     2521 caccaagtat gcaccagccg gtaatactgt atgatgctac atgtaaaggt gccagtagct
//
LOCUS       DAOQQY010000349         2572 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_21891_length_2572_cov_6.370679, whole genome shotgun sequence.
ACCESSION   DAOQQY010000349 DAOQQY010000000
VERSION     DAOQQY010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21891_length_2572_cov_6.370679"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="PLE23_12240"
     CDS             complement(<1..473)
                     /locus_tag="PLE23_12240"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84080.1"
                     /translation="MILWIAFISFIIFALALDLGVFNRNPHEIKTKEATAWTIVWVTI
                     ALLFSGVVYMAFQNNWVGNPTNLTPTQAVLKYITGYLIELSLSVDNIFLIALIFSSFQ
                     IAKKYQHEVLFYGVLGAIIFRAIMIVFGVALINKFSWIIYVFGAFLIFTALKMLLT"
     gene            complement(757..978)
                     /locus_tag="PLE23_12245"
     CDS             complement(757..978)
                     /locus_tag="PLE23_12245"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HMX84081.1"
                     /translation="MAERSGIEIDYCPECRGVWLDRGELDKIIERSIPETNTKSRPAE
                     QHNDRPHYSREDEYKYKNKKSLLGDLFDF"
     gene            complement(1132..1800)
                     /locus_tag="PLE23_12250"
     CDS             complement(1132..1800)
                     /locus_tag="PLE23_12250"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMX84082.1"
                     /translation="MELIFESFQHFLDPDWIMKNGGLYLVLIILFIETGVFFGFTLPG
                     DPLLFISGMVIASVEESHYPFSSEYLNLPFWMVLFMISTILGNYFGYWFGNRFRFLIQ
                     GDKETWLIKRKHIRTATEFYEKRGGLTIAIARFLPIIRTFAPIIAGTVKMDMKKFTYY
                     NILGAVLWVGSLVFLGYLLGDNPWVKANLEYIIIGIIVVVTAPVIIKLFAGSKRNTSG
                     INAQ"
     gene            complement(1985..2275)
                     /locus_tag="PLE23_12255"
     CDS             complement(1985..2275)
                     /locus_tag="PLE23_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMX84083.1"
                     /translation="MQTKEIENGILHEIADDIKAALVADDSLSQIWNKLTPIQRNEWI
                     CWVTIVKKPETRVEHIQRMTEDLKSGKRSPCCWPGCPHRRPEARKWFKNIDE"
     gene            complement(2290..>2572)
                     /locus_tag="PLE23_12260"
     CDS             complement(2290..>2572)
                     /locus_tag="PLE23_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMX84084.1"
                     /translation="LWHGHPVWFIDGNPIVGYSSQKNGIRLMFWSGADFGEGVLNVRG
                     EKFKDASVFYNSANEINTTDLQRWLKKSEEIQWDYKNLIKRKGVLERLK"
BASE COUNT          807 a          511 c          447 g          807 t
ORIGIN      
        1 gtaagtagca tctttaacgc cgtaaatatt agaaacgcac cgaaaacata aataatccag
       61 ctaaacttgt tgataagagc cactccaaac acgatcatta tagcccggaa gatgattgct
      121 ccgagaacac catagaacaa tacttcgtgc tgatattttt tggctatttg gaatgatgaa
      181 aaaatcaagg caatgagaaa aatattgtca acactgagtg aaagttcgat gagataaccc
      241 gtaatatact tcagcaccgc ctgagtaggt gtcagattgg ttgggttgcc cacccagttg
      301 ttctgaaatg ccatatacac gacgcctgaa aacaaaagag ctatcgtcac ccacactatg
      361 gtccaggctg tagcttcctt tgttttaatt tcatgtggat ttcggttgaa aacacccaga
      421 tctaatgcca gggcgaatat aatgaatgag ataaatgcaa tccaaagtat cataaatgag
      481 tttcttggct gtaaaagtaa ttaatttttt ttatttggga atagatgcag attttctgaa
      541 aagacaatta gaaatactgt atgaatggtt gtatgccaat atgttggtta gataaccaat
      601 tgaacacaaa aaagtttttg aaaataaaat aaaagaatca tccagaatat cagatgttat
      661 cgatcatgta tttcatcatc aatatcttta cgaatattca aagaaaaaaa tggggttgag
      721 tatagtcaaa acattaagca caatcacctt tcggctttaa aaatcgaaca aatctcctaa
      781 caaacttttc ttgtttttgt acttgtactc gtcctcacgc gaataatgcg gccggtcatt
      841 atgttgttca gccgggcgcg atttagtatt tgtttccggt attgaccttt caatgatttt
      901 atcaagctca cctcggtcga gccacacacc acggcattca gggcaatagt caatctctat
      961 tccacttctt tctgccatta caagtaggac acttcatatt attaatgatg tttttttgtg
     1021 tttataattc aggcccaata tagattgatt ttttgatttc agtcaatgtg atgtttaaat
     1081 tttacgataa aatgacattc acaaaatagt caagcgtgtt gataacaacg cttattgggc
     1141 atttattccg gatgtatttc gtttgcttcc ggcaaataat ttgatgatta ccggagcagt
     1201 aaccactaca atgattccta taatgatata ttcaaggtta gccttcaccc atggattatc
     1261 acctagtaaa taacctaaaa aaacaaggct accaacccat aatacagcac ccagaatatt
     1321 gtaataggtg aatttcttca tatccatttt gactgtaccg gcaatgatag gtgcaaatgt
     1381 ccggataatg ggcaggaagc gtgctattgc tatggtcaag ccacctcttt tttcataaaa
     1441 ttctgttgct gtacgtatat gttttcgttt gattagccag gtttctttat caccctgaat
     1501 taagaatctg aatctgttgc caaaccaata cccaaaataa ttgcctaata tggtagaaat
     1561 cataaacaat accatccaaa agggaagatt gagatactca gaactaaagg gatagtgcga
     1621 ttcttcgact gatgcaatga ccattccgga aataaagagc aaaggatctc caggtagggt
     1681 aaatccgaaa aaaacacctg tttcgataaa caatattatc agtacgaggt aaagtccacc
     1741 atttttcata atccagtcag ggtctaaaaa atgctgaaat gactcaaaga ttagttccat
     1801 tcaattttta ttaatgtatg aatattgcga agcataacca ttctttgctc atattgttca
     1861 gaaccaaatc ctaaattcga atttggtttc caaaaattta caagttcatg ataaacgttt
     1921 aagctaagaa aaaattggct accctattct gataaaaccg gttaagcatt acgagttgta
     1981 gatttcattc atcaatattt ttaaaccatt tccgagcttc cgggcggcgg tgcggacatc
     2041 cgggccagca acaaggcgaa cgtttacccg acttcaggtc ttcagtcatg cgttggatgt
     2101 gctccactct tgtttcgggc tttttcacga ttgttaccca acagatccat tcatttcgct
     2161 gtataggtgt aagtttgttc cagatttgac tcaaagaatc gtctgccacc aacgctgcct
     2221 ttatatcatc tgctatctca tgcagaattc cattctctat ttcttttgtt tgcattttgt
     2281 gtcggtttgt tacttcaaac gttcaagaac gccttttcgt ttaatcagat ttttataatc
     2341 ccattgtatc tcttcagatt ttttcaacca tcgttgcaaa tcagtagtat ttatttcatt
     2401 ggcagaatta tagaaaactg aggcatcctt aaatttttca ccccgtacat tcaatacacc
     2461 ctcaccaaag tcagcaccac tccagaacat tagcctgatt ccgttttttt ggctgctata
     2521 gccgacaatc ggattaccgt caatgaacca taccgggtgt ccgtgccaca at
//
LOCUS       DAOQQY010000350         2561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_22043_length_2561_cov_5.724262, whole genome shotgun sequence.
ACCESSION   DAOQQY010000350 DAOQQY010000000
VERSION     DAOQQY010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22043_length_2561_cov_5.724262"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLE23_12265"
     CDS             <1..253
                     /locus_tag="PLE23_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HMX84085.1"
                     /translation="KSAKGSCGCTVPSYKKDPVMPGESGEVEVRYDTQRVGPFSKRVT
                     LETNEDIGSRGLTIKGEVRKTVPDNPVPAVSPNVLQGNK"
     gene            complement(654..1706)
                     /locus_tag="PLE23_12270"
     CDS             complement(654..1706)
                     /locus_tag="PLE23_12270"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMX84086.1"
                     /translation="MSNEAKIGILAVVAILISIFGYKFLKGQEVFSRTEEYKVEYANV
                     DQLTKAAPVLINGFQVGAVKDIYIKPEDQRTIVVTISIKGGVKIPKNTIAAIKPSGLV
                     GGKVIELYFSSPCQGGDCAVNGDYLKGVTNGLLDAIVGKEQLDSYLTQLKDGLTGIYD
                     TLSEKASDPNAKRGLPKTINDINVIMENLKATTQDLKVLIALSSQSIPSITKNLDDIT
                     KNLKANNDKITAILNNTAKFSENLSKTDISATNTSLQTSLTSLDQTLKSTDLAVKDLK
                     KITDGINNGSGSLGLLVKDPQLYNNLNKTSRNLDLLLQDLRLNPKRYINVSVFGKKQK
                     EYTLPEADPATVPSNN"
     gene            complement(1773..>2561)
                     /locus_tag="PLE23_12275"
     CDS             complement(1773..>2561)
                     /locus_tag="PLE23_12275"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMX84087.1"
                     /translation="NLGVAKRENSAILLEYDYKANYDGFDPNAPETHIMLSMFQNAYL
                     GQSISFAQAFELEAAAHAKRKSRGVRQAGFLVLKATTMPSVLIETGFLSNENEKDFLV
                     SDEGQNKIAAAIARAFYNYKAHIEDEQPIVAGKQMNLQLNLGTNAVKKEQALVQNENS
                     KQNTSPIAAPTTITFRIQLLASQNPVDTNVNNIFKDILGIQVVKEGNMYKYYSRDFDT
                     PEAMLPEKEKMRKAGFKGAFTVAFENGVRIKLEDAKKKIDRTGK"
BASE COUNT          665 a          507 c          521 g          868 t
ORIGIN      
        1 caaatcagca aaaggaagtt gtggttgtac tgtaccgtct tacaaaaaag acccggtaat
       61 gccgggcgaa tcaggtgaag tagaagtaag atacgacact caaagagtag gtcctttcag
      121 caaaagagta acgcttgaaa ctaacgaaga catcggatca agaggtctta ctatcaaagg
      181 cgaggtaaga aaaactgttc ctgacaatcc ggtacctgca gtatcaccta acgtgctaca
      241 gggtaacaag taagaataat tgtttttctt cataaagaga gctgctctga tttttcggag
      301 tagctttttt tattagcctc aatccacttt ttgactctta ctgcaaatag gtattttgca
      361 aatccatttc aacagctcaa aaatgctttt atctgtttat taaggattat ttgcactaat
      421 tattttcgtt taatttctta atgcattatc cgggttaaaa ttatttacta catcttcccg
      481 ttaattttta tctcatccta catattcatt gcttcactgc gatgttcggg acaagactgc
      541 actttatttc tttttctaga atgatgacat gtacaaacaa gctcattagt ttttgtgcaa
      601 aaacaaaagc tcgctaaaat gtcggtcgat gaatatactt agtagcgcag gtattaatta
      661 ttggatggta ccgtggccgg gtcggcttcg ggcaatgtat attctttttg tttcttgcca
      721 aatacgctca cgttaatgta gcgtttcgga ttaagtctga gatcctgcag aagcaaatcg
      781 agattgcggg atgtcttatt caggttattg tacaattgag ggtctttgac cagcaagcca
      841 agagatccag aaccattatt gattccgtcc gtgatttttt tcaagtcttt tactgcaaga
      901 tcggtacttt taagtgtttg gtcaagagat gtaagacttg tttgcaagct ggtgttggta
      961 gctgaaatgt ctgtcttgct caagttctca gagaattttg cagtattatt cagaatggct
     1021 gttattttat cgttattagc cttaaggttc tttgttatgt cgtccagatt tttggtaatg
     1081 gatgggattg attgcgagct cagggcaatc agtactttta ggtcttgggt tgtagccttg
     1141 aggttttcca ttattacatt tatgtcgttt atggtttttg gtaggccacg ctttgcattg
     1201 ggatcagagg ctttttcact cagggtgtca tatataccgg taaggccgtc cttgagctgg
     1261 gtcaggtacg aatcaagctg ttctttaccg acgatagcat ccaggagtcc attggttact
     1321 ccttttagat aatcaccatt tacagcacag tctccacctt ggcaaggact tgaaaaatat
     1381 agttcgatga cctttccgcc tacgagtccg ctgggtttga tggctgctat cgtattcttc
     1441 ggtattttga caccaccttt gatactaatg gttaccacaa tggtgcgttg atcttcgggt
     1501 tttatataga tatccttaac cgctcctacc tgaaagccgt tgatgagcac aggagcagcc
     1561 ttggtcagct gatcgacatt ggcatattct accttatatt cttcagtacg actgaatacc
     1621 tcctgacctt tgagaaattt gtatccgaaa atcgaaatca ggattgccac cacggccaga
     1681 atccctattt ttgcttcatt tgacatataa aagattcaaa aaacacaaca attggtgaca
     1741 aagataaaat attgtaagca ttatcaatta gtttatttac ctgtccgatc aattttcttt
     1801 ttggcatctt cgagcttgat tcgcacgcca ttttcaaagg ctacggtaaa tgctccttta
     1861 aatccggctt ttcgcatctt ctccttttca ggtagcattg cttcgggtgt atcaaaatca
     1921 cgtgaataat atttatacat attaccttcc tttactactt gtatgcccaa tatgtcttta
     1981 aaaatgttgt taacattggt atcgaccgga ttctggcttg ccagcaattg aattctgaat
     2041 gtgatggttg taggtgctgc aatgggagaa gtgttctgtt tgctgttttc gttttggaca
     2101 agtgcttgct ccttctttac agcattggtt ccaaggttga gttgcagatt catttgtttg
     2161 cctgctacaa tgggttgttc atcttcgata tgagctttgt aattgtaaaa tgctctcgca
     2221 atggcagcag caatcttatt ctgaccttca tcactcacta ggaaatcctt ttcattttca
     2281 ttcgacagaa acccggtttc gattaataca cttggcatag tagtggcctt caatacaagg
     2341 aaacctgcct gccgtacacc acggcttttt ctcttagcat gagccgctgc ttcgagttcg
     2401 aatgcttggg caaatgatat actttgaccc aaatatgcat tctgaaacat agataacatt
     2461 atatgtgtct cgggggcatt cgggtcaaat ccgtcgtagt tcgctttata atcatactcc
     2521 agcaggatgg cactgttctc acgttttgct actcccaggt t
//
LOCUS       DAOQQY010000351         2555 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_22146_length_2555_cov_3.976400, whole genome shotgun sequence.
ACCESSION   DAOQQY010000351 DAOQQY010000000
VERSION     DAOQQY010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22146_length_2555_cov_3.976400"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /locus_tag="PLE23_12280"
     CDS             complement(<1..1276)
                     /locus_tag="PLE23_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84088.1"
                     /translation="MKSLKLTGFVFLLLLTTSLVNAQLTQKSTPASWRNLPQLTAMDQ
                     LPVFSPQLPNISLAIKEDETSGQDRFALPVTWGDNPLDNGIWEKAEGILIWRMKINAN
                     ESKGWIVCLNKVNIPDGGSLFIYSANTKILKGAYTSSKNNLADKLTIGPVSGSDIIIE
                     YNIPDKVEAQLPFLLDKLYFVYKENPNVPIQQQSLLGDPAYQSSWPCMINVNCPEGSQ
                     YGKEKNGVVRILIASDKGYYYCTGSLVNNTKKDRTPYILSAFHCEHGLVPYYDNYIFA
                     FGWETADCTTPVESPEYQTMTGCDLVARREESDFTLYKLKTDIPVLINAYFNGWNKSA
                     LKKPSKTATIHHPNGDVKKISLDNNPAVVWASPINWAYGATTPAGHHWRTGLSKGASM
                     PGSSGAPLFDENKMIVGQLHGGSSTCSLTTLFY"
     gene            complement(1335..>2555)
                     /locus_tag="PLE23_12285"
     CDS             complement(1335..>2555)
                     /locus_tag="PLE23_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMX84089.1"
                     /translation="SNMGITEDLHYQLMHQLNGTQKVKVLLAQALFGNPDILLLDEPT
                     NDLDPETIMWLEDFLADYRNLVIVVSHDRHFLDMVCTHVVDIDFGKIKIFSGNYSFWY
                     ESSQLALKQMLDKNAKLDKKRQEMMEFIQRFSANASKSKQATSRKKALDKLNLEEIQP
                     SSRKYPFIFFKSEREAGNEIFKCNNLGKKNLNGEWLFRNVNVQFNKGDKIGLVSKDSV
                     ATTAFYNVIAGIDQPDEGNYEYGQTITKDYLPVENADYFAVEHDLDLINWLRQYSKEK
                     DEQFIRGFLGKMLFSGEEVYKMVSVLSGGEKVRCMLSKIMLSGPNFLLVDEPTNHLDL
                     ESITAFNNALTAFPGSLFITSHDHRFMQTVANRVVEISPNGMMDKLMDYDEFLASDAV
                     KEQRRQLYGESVVV"
BASE COUNT          675 a          533 c          543 g          804 t
ORIGIN      
        1 cgtaaaaaag ggtagttaac gagcaggtag aactacctcc gtgaagctga ccgacaatca
       61 ttttgttttc gtcaaacagg ggcgctcctg atgaccctgg cattgatgct cctttgctca
      121 gccctgtgcg ccagtggtgg ccggctggtg ttgtggcacc ataagcccag tttatagggc
      181 ttgcccatac taccgcagga ttattgtcta aagaaatttt cttaacatcg ccattcgggt
      241 gatgaatggt ggcagtcttt gatggtttct tgagtgctga tttattccag ccattgaagt
      301 aagcatttat cagtaccggt atatctgttt tcaacttata aagtgtaaaa tcgctctctt
      361 cccttctggc cacaaggtcg caaccggtca ttgtttggta ttcgggtgat tctaccggtg
      421 ttgtacagtc ggccgtttcc catccgaatg caaaaatgta attgtcataa tatggcacca
      481 aaccatgttc gcaatggaat gcagataata tataaggtgt acggtctttt ttggtattgt
      541 taaccaaaga acctgtgcaa taataataac ctttgtctga agctatcaat atgcgtacta
      601 caccgttttt ttcttttcca tattggctgc cctccgggca atttacatta atcatgcaag
      661 gccaggatga ctgatatgcc ggatcaccaa gtaaggattg ttgttggatg ggcacattag
      721 gattttcttt atagacgaaa taaagtttat caagtaaaaa tggcaattgg gcttctactt
      781 tatccggtat attatattca atgatgatgt cactaccaga caccggaccg attgtaagct
      841 tatcagccag attgttcttg gatgatgtgt aggcgccttt gagtattttt gtattggccg
      901 aatatatgaa caagctgcca ccgtctggta tgttgacttt attcaggcag acaatccagc
      961 cttttgattc attggcattt atcttcatac gccatatcaa aataccttct gctttttccc
     1021 agattccatt gtccaaagga ttatcacccc atgtcaccgg caatgcaaat ctgtcttggc
     1081 cacttgtctc gtcttccttg attgcaagag aaatattagg tagctgcgga gaaaaaaccg
     1141 gcaattgatc cattgcagta agttgcggca aatttctcca cgaggcagga gttgactttt
     1201 gtgtaagctg tgcattaacc agactggtcg tcagtaataa caaaaacaca aatccggtta
     1261 attttaggct tttcattgat atgtcgggta tgttgttgaa aaaatcagtt tatatgacaa
     1321 aaaagcgtgc cattctaaac aacgactgat tcgccatata attgtctgcg ttgttctttg
     1381 actgcatccg atgccagaaa ttcatcgtaa tccataagtt tgtccatcat accatttggc
     1441 gatatctcca ctacacggtt agcgactgtt tgcataaacc ggtggtcatg agaggtaatg
     1501 aacagactac ccggaaaggc agtcagtgca ttattgaatg ctgtgattga ctcgaggtcg
     1561 aggtggtttg ttggttcgtc cacaagcaga aaattagggc ccgaaagcat gatttttgac
     1621 agcatacatc tgaccttttc accacccgac agtacactca ccatcttgta aacttcttct
     1681 cctgagaaca acattttacc caaaaatccg cgaataaatt gttcatcttt ttctttagaa
     1741 tactgtcgca accagttaat cagatcaagg tcgtgctcga ctgcaaaata gtcggcattc
     1801 tctaccggaa ggtaatcttt tgtaatggtt tggccgtatt catagttgcc ttcatccggc
     1861 tgatcgatac ccgcaattac attgtagaat gctgtagtag caacactgtc ttttgatacc
     1921 agaccaattt tatcgccttt gttaaactga acattcacat tgcgaaataa ccactcgcca
     1981 tttaggtttt tcttaccgag attgttgcat ttgaatattt cattaccggc ttcacgctcc
     2041 gatttaaaga aaataaaagg atattttcgg ctactgggct gtatttcttc aaggttgagt
     2101 ttgtcaaggg ctttctttcg gctggtagcc tgctttgatt tggatgcatt ggcggaaaat
     2161 cgctggatga attccatcat ttcctgtctt ttcttgtcga gtttggcatt tttatccagc
     2221 atctgcttga gggcaagttg actcgactcg taccagaaag agtagttacc actgaatatt
     2281 tttattttac caaaatcgat gtccacaaca tgtgtacaga ccatatctag gaaatgtctg
     2341 tcgtgcgaaa ccacgatgac tagattgcgg taatcggcaa gaaaatcttc gagccacatg
     2401 atggtctccg gatcaaggtc gttggtaggc tcatcgagta gcagaatatc agggttgcca
     2461 aaaagggctt gtgccaatag tacctttact ttttgagtac cattgagttg gtgcattaac
     2521 tggtagtgga ggtcttcggt gatgcccata ttact
//
LOCUS       DAOQQY010000352         2552 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_22192_length_2552_cov_5.975971, whole genome shotgun sequence.
ACCESSION   DAOQQY010000352 DAOQQY010000000
VERSION     DAOQQY010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22192_length_2552_cov_5.975971"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PLE23_12290"
     CDS             <1..816
                     /locus_tag="PLE23_12290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84090.1"
                     /translation="NSGNLGKIFEIKYLIENLKNLAEFNTYREHHKDIISQKEALTEA
                     ELLVHTSLTPGAKDTAMQILNTQIAVLDSMLNESQALADDMQLAFSTAQAEMQSIRES
                     LSNMNQIEQNYSIVYGIVIDHLNDTEFSLSQNETDSLYYVAEQCYGTGGRAVLDARHL
                     LRILGLEASYDDDCFIEPRGGGRQYDEEPSTSIDIIYTNPASRLVKYQIKGEYTDGGM
                     EIIIINLTGNVVGRELLTKNKGEFDISSLSSGLYMLVCRVGKEIATHKLVVQY"
     gene            842..>2552
                     /locus_tag="PLE23_12295"
     CDS             842..>2552
                     /locus_tag="PLE23_12295"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX84091.1"
                     /translation="MRFWLGLILIVYCVVSSVYSQNYDNVWVLGQRSAILPPENDTSK
                     NNFYIDFKNDSFKLVKKYELQTEIMNDGTSICDSLGDLIMFTNGCAIYDSEGQIISNG
                     DSINYLPDGGNNSWVSFCSEGEGYPRYRGTRFIPFFSLNRIFLLHTAFSDEFEVSYRY
                     LLYTEIEFKNGVWKVTSKNNLLYDQAKTGIRYDITKHANGRDWWLVAHKMGDKEFLEW
                     LITPEGINFQSNQSFQHPVPEDSLDPTFSPDGTKLVQHNLVGDSWIINFDRCTGTLSE
                     PYKLPRPKGAWYWSQLAFSPNGCFIYFADSYHLYQIDLLDTDTLSNIETISVWDELSW
                     PCDVGCYTDYNVLEPGPDGKIYGGAITMSKVVHVIERPNEKGKACMFRPRAYKTPFFI
                     HGRLPAYPYYRLGPIDGSACDTLGINNVPVAKFRYYPDSVDWHKIEFVDNSYYNPTDW
                     YWDFGDGSGNSSTEVNPVYSYAKSGVYDVCLTVSNAYGSDTYCRYVRVGTVSTTEPNA
                     ERYMKLYPNPATNSINIELGEKLSDGRAYTIHCYDLLGRRCYTGTLPAYSYLHTIDVS
                     AFAP"
BASE COUNT          785 a          434 c          579 g          754 t
ORIGIN      
        1 aattccggta atcttggaaa gatttttgag attaaatatc taattgaaaa tctaaaaaat
       61 ttagcggagt tcaatactta cagagagcat cacaaagata ttatctcaca aaaagaggct
      121 ttaactgaag cggagctatt ggttcataca tccttaacac caggtgcaaa agataccgcg
      181 atgcagattc tgaacaccca gatagcagtt ttagattcaa tgttgaacga aagtcaggct
      241 ttggcggatg atatgcagct ggctttttca actgcacagg ctgaaatgca aagtatcaga
      301 gagtcattga gcaatatgaa tcagatagaa caaaactatt cgatagtata tggtattgtg
      361 atagatcatt tgaacgatac ggaatttagt ctgtcgcaaa atgaaacaga cagtttgtac
      421 tatgtagcgg aacaatgcta cggtacagga ggaagagccg tgctggatgc ccggcattta
      481 ctgcggatat taggtttaga agcatcttat gatgatgatt gctttattga gcctcgagga
      541 ggtggaaggc agtatgatga ggaaccttcg acttcaattg atattatcta tacgaatcct
      601 gcttcaaggt tggtaaaata tcagataaaa ggagagtaca ctgatggagg aatggaaatt
      661 attattataa acctaacagg aaatgttgtc ggcagagaat tgctcacaaa gaataaaggt
      721 gaattcgata tttcaagcct ttcttccggt ttgtatatgc tagtttgtcg tgtcggaaaa
      781 gaaattgcaa cgcacaaact agtggttcaa tattaattaa gagttgttaa aattccttat
      841 tatgagattt tggttagggc ttatcttgat agtgtattgt gttgtttctt cggtttatag
      901 tcagaattat gacaatgttt gggtgttagg ccaacgctca gctattttac ctccagaaaa
      961 tgatacatca aaaaataatt tttatattga ctttaaaaat gacagcttta aattggtaaa
     1021 aaaatatgaa ttacaaacag aaataatgaa tgatggtact tccatttgtg attcattagg
     1081 tgatttgatc atgtttacca atggttgtgc aatatatgat tctgaaggac aaatcatatc
     1141 aaatggtgat agtatcaact atttaccaga cggtggtaac aattcttggg tatcattttg
     1201 tagtgaagga gagggttacc cacgatacag gggtactcgt ttcatacctt ttttctctct
     1261 gaataggatt tttttattgc atacggcatt ttcagatgaa tttgaagtta gttatagata
     1321 tttactatac acagaaatcg agtttaagaa tggagtttgg aaagtcacgt ccaagaataa
     1381 tcttttatac gatcaagcta aaacaggaat acgttatgat ataaccaagc atgcaaacgg
     1441 gcgagattgg tggttagtgg cacataaaat gggagataaa gagtttttag aatggttaat
     1501 aactccggaa ggtattaatt ttcaaagcaa tcagtcattt cagcatcctg tacctgaaga
     1561 tagtttggac ccaacctttt ctcccgatgg aacaaaactt gtccaacaca atctggtggg
     1621 cgattcttgg ataattaatt ttgatagatg cacaggaacc ttaagcgagc catataagct
     1681 tccccgtcca aaaggggcat ggtattggag tcagcttgct ttttctccga atgggtgctt
     1741 tatatatttc gcggatagct accatttgta ccagatagat cttttagata cagatacact
     1801 gagtaacata gaaaccattt cggtttggga tgaactgagc tggccttgtg atgtaggttg
     1861 ttatactgat tataatgtac ttgagcctgg ccctgatggg aaaatatacg gtggcgctat
     1921 aacaatgtca aaagttgtcc atgtcataga gcgcccgaat gaaaagggca aggcttgtat
     1981 gttcaggcct cgtgcataca agacaccctt ttttatacat ggtagattgc cggcttatcc
     2041 ttattaccga cttggcccga tagacgggag cgcctgtgat accttaggca ttaacaatgt
     2101 tcctgttgct aagttcaggt attatcctga cagtgtagat tggcataaga tagagtttgt
     2161 agataattcg tactataatc ctacagactg gtactgggat tttggtgatg gaagcggcaa
     2221 cagttcgacg gaagtaaacc ctgtatatag ttacgcaaaa agtggcgttt atgatgtatg
     2281 cctgacggtg agcaatgcct acggcagcga cacatactgc aggtatgtgc gtgtaggtac
     2341 tgtgtccacc acagagccga atgcagagag atatatgaag ctatatccga atcctgccac
     2401 gaacagcatc aatatagagc tgggtgagaa gctgtccgat ggacgtgcct acaccatcca
     2461 ctgctacgac ctgctgggca ggcgctgtta tacgggtacg ctaccggcgt attcgtacct
     2521 gcacacgata gatgtatcgg ccttcgcccc gg
//
LOCUS       DAOQQY010000353         2549 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_22244_length_2549_cov_6.449479, whole genome shotgun sequence.
ACCESSION   DAOQQY010000353 DAOQQY010000000
VERSION     DAOQQY010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22244_length_2549_cov_6.449479"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLE23_12300"
     CDS             <1..235
                     /locus_tag="PLE23_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84092.1"
                     /translation="SFTGIDPKAQSISLQMGKTKFDKKSFGIDVAENYERSDFIVLEA
                     VLFPGINLVWGGTLIMLLGLLVSAFKRYQDQKV"
     gene            263..1042
                     /locus_tag="PLE23_12305"
     CDS             263..1042
                     /locus_tag="PLE23_12305"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HMX84093.1"
                     /translation="MKVSVLGAGNVAFQLLKVFSAKGVQVVQLYNRTRSKAEILAGEY
                     AIPFTDKLEELSTDVDIYIICVSDDGIIPFASKLKAFIGNKLVAHTSGAVDTNVLEPY
                     FKNYGGLYPLQTFTINTIPDFEKIPVMVAGSTPENIELLKRLAKQISSKVAIVNDRER
                     LMYHLAAVIANNFSNYLFGQASNLLKEHRLDFSMLLPLIEETVRKIHTADPVCIQTGP
                     ARRGDLKTISAHLALLGDHPELKKIYIDISNSINSELNIKQ"
     gene            1039..1320
                     /locus_tag="PLE23_12310"
     CDS             1039..1320
                     /locus_tag="PLE23_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84094.1"
                     /translation="MRIIGYLEHPGMVVTVFKNDNKISVKFEKNLLELVFKVREDLHL
                     SDVAEVNKLFDAEFMVKIEAIFQEMAAARDNALLRYQSNVSTGSEPDIF"
     gene            2304..>2549
                     /locus_tag="PLE23_12315"
     CDS             2304..>2549
                     /locus_tag="PLE23_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84095.1"
                     /translation="MQKFLLWVALLLCNIVILNATTSTGANTKHHNDYVRPVVIKNVH
                     SGMKKLFNYRTNTGNANFGRYTAKSSVKAGNAIISNTY"
BASE COUNT          885 a          441 c          440 g          783 t
ORIGIN      
        1 gagttttaca ggtatcgacc cgaaggcaca atctatttcg ttacaaatgg gaaaaacaaa
       61 attcgacaaa aaatccttcg gaatagatgt agcagaaaat tatgaacgtt ccgatttcat
      121 tgtactcgaa gcagtcctat ttccgggaat caatctggtg tggggcggta cacttataat
      181 gctgttaggg ctgctggtaa gtgcatttaa acgttatcaa gatcagaaag tataggcgca
      241 gttatgaatc aatgaatgct atatgaaagt ttcagttttg ggtgcgggca atgtggcttt
      301 tcagctgtta aaagtatttt cagccaaagg tgtccaagta gtacaactat acaaccgtac
      361 gagatcaaag gccgaaatac tggctggtga atacgccatc ccctttaccg acaagcttga
      421 agaactttca acagacgtcg atatctacat catctgtgtt tcggatgacg gtattatacc
      481 atttgcgagc aaattgaagg cttttatagg aaataaactg gtagcacaca cttcaggtgc
      541 tgtagatacg aatgtacttg agccatactt taaaaattat ggcgggttgt acccattgca
      601 gacttttacg ataaacacca tacccgattt tgaaaaaata cccgttatgg tagccggcag
      661 tacgccggaa aatattgaat tacttaaaag gcttgctaag caaatatctt caaaggtggc
      721 aatagtcaat gatcgtgaac ggcttatgta ccacttggct gcagttattg ccaataattt
      781 tagcaattat ctttttggcc aggcatcaaa cctactcaag gaacacagac ttgacttcag
      841 tatgctcctg ccactcatag aagaaacggt cagaaaaata cacaccgctg accccgtttg
      901 tattcagacc gggcctgcac gaagaggcga tttgaaaacc ataagtgcac atcttgcctt
      961 gttaggcgat catcctgagt tgaaaaaaat ttatattgat atatccaatt caatcaattc
     1021 agaattaaac ataaagcaat gagaataatt ggttatctcg agcacccagg tatggtggta
     1081 acagtgttca aaaacgacaa taagatttct gtaaaatttg agaagaatct tctcgaactc
     1141 gtattcaaag tgcgtgagga tcttcattta tcggatgtag cagaggtcaa taaacttttc
     1201 gatgccgaat ttatggtaaa aattgaagcc atctttcaag aaatggctgc tgcgagggat
     1261 aatgcactgc tgcgatacca aagtaatgta tcaaccggaa gcgagccgga tatattttag
     1321 tatagttgaa tcatcattgt ccttgaaacc atctacatac aatcacagga tgtgtcccgt
     1381 tgttcatgat tcatttaaat ttagaaaata aaaaatcatt tccaaagcct gtattttttg
     1441 ccaaacaaaa taaagataag ctcattcagg taatctgttc acataataat tttacaacat
     1501 attgaacgta ctgaatctga actaaataat aaaaacctta aattatttaa caatattatt
     1561 caagacaaat taatcttggt gtgtgcattt acatcaatga tattgaatga tatttttcat
     1621 ctctttttaa ttgaaataat ggcaatattt ttcacccatt gaatagtcac taatatgatc
     1681 acaaaattaa aatttaagaa atgccgtaaa aaatagcatt tatattgatt aaattataat
     1741 ttatctgaac tcacatcttt ttcaaaaatt aattctcatc gttttaaaca tatttttttt
     1801 atgaaattat aattatttca ttgtattata aaattaattt aaaagaatgt taagtcaatt
     1861 tttatttttt tttgctcgtc acattaaatc tacatccagc tatcaaaaat agctatgcct
     1921 catgaatctt tgcaacagat ataaatgaaa ctcgagaggt agaactcttg aggattaagt
     1981 cctttaataa aaaacaatag atgagaaacc ttctgtacat caaagcatta tggtctgtaa
     2041 atgacgatat gtctgaacag ttgcatgctg ttcctgtctg tgacaagtac gaggttaaac
     2101 atagaatgat ttggtaaagg atattatcaa atcatttttt tattgttgaa atattaaaga
     2161 aatttttaat tttacatccg ttttcccatc acctaccagc aagaaaataa catacgaaaa
     2221 ccggaatcaa acgaaagtat taaaaacata accatcccag aaaccgcgct gatttcgaga
     2281 atatttttat taaaaccatg attatgcaaa agtttctact ttgggtagca ttattattat
     2341 gtaatatcgt tattctaaat gcaacaactt caactggggc aaacactaaa caccacaatg
     2401 actatgtaag acctgtggtg attaagaatg tgcattccgg catgaagaaa ctatttaatt
     2461 atcggacgaa taccggaaat gccaatttcg ggcgttatac agctaagtca tcagtcaaag
     2521 ctggaaatgc cattatatct aatacgtat
//
LOCUS       DAOQQY010000354         2537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_22434_length_2537_cov_5.171233, whole genome shotgun sequence.
ACCESSION   DAOQQY010000354 DAOQQY010000000
VERSION     DAOQQY010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22434_length_2537_cov_5.171233"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..892)
                     /locus_tag="PLE23_12320"
     CDS             complement(140..892)
                     /locus_tag="PLE23_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMX84096.1"
                     /translation="MNKHIYTAVLIDDEEHCIKTLKWNLTEFCPEIEIIGEANTARDG
                     YSIINQLNPDLIFLDVEMPDMSGFDMLAKFDKINFKVIFTTAYNEYAIKAIKLNAIDY
                     LLKPIDKDELKAAIVKLHTANSDVLHSKIQNLKFNLHVSPEQQRITVSTIDGVQFFDL
                     KAITCLEADSNYTYLHFNDGSKILSSRTLKEYEEVLPPDSFFRCHNSFIVNFRFIKKY
                     TKGEGGEIELVTGQKIAVSRRKKQEFIDWITM"
     gene            complement(927..>2537)
                     /locus_tag="PLE23_12325"
     CDS             complement(927..>2537)
                     /locus_tag="PLE23_12325"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMX84097.1"
                     /translation="KCKDTGQYLRNNIIRVRYAEEKENGDVYLATENNGLVRYNYING
                     RFTEFPSIRRISGVCRHFILDQGKIWAGTEDGLVYLDPVTGKVLQHLTTENGLSDNVI
                     YGVLMDDSKQLWVSTNKGICKIDQKLSKVTIYSMEDGLQDLEFNTAVAYKFTNGNLLF
                     GGINGFNIIKPVELKKINFVPTPLLVDLKVLNIPYSDSVPYPFLKNISLKFNQNFITM
                     GFQSPVFTQINRINYRYMLSNLHETWINNGSRNYVNFTQLKPGNYTFKVQAFDDNKVY
                     SGINEIYITIHAPWYYNFWFLFFSSMAIVTSSLLIISARIKALKVRKSLLHQKAVAEM
                     QSLRLQMNPHFIFNSLNSINSYIVDNKTLQASDYLSKFSRLMRLILENSRHETITLSK
                     ELEYVSLYLLIESLRFAKAFEYEIHVDESVDAENLIVPPLFIQPFLENSIWHGLMPRK
                     GTRTLNLDLTQSSDILTVVVSDNGVGREYSAKLKETKITLKNSHGMDITKNRIMYHNP
                     KNTVQIEDLYNALGEAAGTRVVISIHYI"
BASE COUNT          734 a          463 c          465 g          875 t
ORIGIN      
        1 ctctttactg taaataatta ttacttcaaa tccatttcaa aagctcaaaa accatcgttc
       61 cctgtgcttt tatcacttat tttggatttt tttgtacgaa tcatttccgt ttaaattcct
      121 aatgtgttat ctggtaaaat cacatcgtta tccagtcgat gaattcctgt ttttttcttc
      181 tcgagaccgc tattttctga cctgtgacga gttctatttc gccaccttcg ccttttgtat
      241 attttttgat aaacctaaaa ttgacgatga aagaattatg gcatctgaaa aaactatccg
      301 gaggcagaac ttcttcgtac tctttgagtg ttctggaaga aagaattttt gagccatcgt
      361 tgaagtgcaa atatgtataa ttactgtcag cttccagaca ggttattgcc ttgaggtcaa
      421 aaaactgtac gccatcgata gtacttacag taatgcgttg ttgttcgggg ctgacatgca
      481 ggttaaattt caggttttgg attttgctgt gcaatacatc actgtttgca gtgtgcagtt
      541 taacaatggc tgcttttaat tcatccttgt ctattggttt caacaggtag tcgatggcat
      601 tgagtttgat ggcctttatg gcatattcgt tgtaggctgt ggtaaaaatg actttaaagt
      661 tgattttatc gaatttggcc aacatatcga aaccactcat atcaggcatt tcaacatcta
      721 aaaatatcaa gtcgggattg agttgattga tgattgaata tccgtcacgt gctgtgttgg
      781 cctcccctat tatttctatt tcgggacaga attcggtcag gttccattta agtgttttga
      841 tacaatgctc ttcatcatcg attaatacag ctgtatatat atgtttattc attagaaatg
      901 aaaattgaat gtttataaat aattatctaa atgtagtgaa tactaatgac gactctggta
      961 cctgctgctt cgcctaaagc attatacaag tcttcgatct ggacagtatt ttttggattg
     1021 tggtacatga tgcgattttt tgtaatatcc attccatgtg aatttttaag cgttattttt
     1081 gtttccttaa gttttgctga atattctcta ccaacgccat tatcggagac cacaacagta
     1141 agtatgtctg aagattgagt caggtctagg ttcaatgttc gggtaccctt acggggcatc
     1201 aatccatgcc agatagaatt ttccagaaaa ggctgaataa acagaggtgg cactattaga
     1261 ttttctgcat ctaccgattc gtcaacatga atttcgtatt caaatgcctt tgcaaaccga
     1321 agtgattcaa tcaaaagata caagcttaca tactcaagtt ctttgctcag agttatggtt
     1381 tcgtgtcgcg aattttcaag aataagcctc ataagccttg aaaacttaga caaataatca
     1441 cttgcctgta aggttttgtt atcaacgata taactattga tactattgag cgagttaaag
     1501 ataaaatgcg gattcatttg taggcgcagt gattgcattt cggctacagc tttttggtgt
     1561 agtagtgatt tccttacttt caatgccttt attcttgccg aaataatgag aagagaagat
     1621 gtaacgatgg ccattgaact aaagaataga aaccagaaat tataatacca cggagcatgg
     1681 attgttatat atatttcatt tatgccggaa taaactttat tgtcgtcgaa ggcttgtacc
     1741 ttaaaggtat agttgccagg tttgagttgt gtaaaattga cataatttct tgaaccgttg
     1801 tttatccagg tttcgtgcag atttgacaac atatacctgt agtttattct gttgatttgc
     1861 gtaaaaaccg gactttgaaa tcccatcgtg ataaaattct ggttgaattt caaagaaata
     1921 ttcttcagaa aaggataagg tacactatca gaatatggaa tattcagcac ttttaaatca
     1981 accaataacg gggttggcac aaaattgatt ttctttaatt caaccggttt gatgatatta
     2041 aatccgttga taccaccaaa caataggttt ccgttggtaa atttgtaagc tactgcggta
     2101 ttgaattcaa gatcctgcaa gccatcctcc atcgaatata tagtaacctt tgacagcttt
     2161 tgatctattt tacaaattcc cttgttggta gatacccaca actgcttcga atcatccatc
     2221 aatacaccat agatgacgtt atctgacaat ccattttcgg tcgtgaggtg ttgtaatact
     2281 tttccggtaa caggatcaag atatactaag ccatcctcag tacctgccca tattttgccc
     2341 tgatcaagaa taaaatgccg acaaacaccc gatatcctgc ggatggaagg aaattcagta
     2401 aatcgaccat tgatgtaatt gtacctgacc agaccgttgt tttcggtagc cagatacaca
     2461 tcaccattct ctttttcttc tgcataacgc actctgatga tattattacg caggtattgt
     2521 cctgtgtctt tacattt
//
LOCUS       DAOQQY010000355         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_22695_length_2520_cov_7.556592, whole genome shotgun sequence.
ACCESSION   DAOQQY010000355 DAOQQY010000000
VERSION     DAOQQY010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22695_length_2520_cov_7.556592"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="PLE23_12330"
     CDS             complement(<1..306)
                     /locus_tag="PLE23_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84098.1"
                     /translation="MKNGKQNTNQRTSFFRLLTAFMLVAWTTTFSWSQIANAPCNLES
                     PWIQGVGPGTYTNLEAKINDNVFSLLPPCLGSVSNVGNLIDGNWTSSSTWKNGNIPNA
                     "
     gene            complement(1146..1835)
                     /locus_tag="PLE23_12335"
     CDS             complement(1146..1835)
                     /locus_tag="PLE23_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMX84099.1"
                     /translation="MAYYLILLCMDVLSTLYPNMNPDRTQKSLLVLDDSEIIYWGIVM
                     LCSRNFPDCRVYYADSGDDCIKKLREKHFDVILMDVNILFTDTSQVLRLIRLNFPLIR
                     VVIFSNEKAISMHFVDKGADGFIRKSSKSIDLISQLGKVIYGENINKALLDLDEGIPI
                     PIDRLSLLTPREYEIMNFLLKGYKTTEISAILKLQKTTVSTHKAKIFQKLNVSNTLEL
                     KAVTDSIRQIS"
     gene            1997..2338
                     /locus_tag="PLE23_12340"
     CDS             1997..2338
                     /locus_tag="PLE23_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HMX84100.1"
                     /translation="MIKVKRIYETATYSDGYRILVDRLWPRNMTKEHAAIGIWMKDLA
                     PSTELRKWFNHEMEKWPEFEQKYKAELKAKQQYLQQLRQLEKEHGTITLLYAAKDEKH
                     NNAQVIVEWLQ"
BASE COUNT          899 a          425 c          406 g          790 t
ORIGIN      
        1 agcattagga atgtttccgt tcttccatgt gcttgaagat gtccagttgc catcaataag
       61 attaccgaca ttactgaccg accctaagca aggtggaaga agtgaaaata cgttgtcgtt
      121 aatttttgcc tccagattgg tgtaggtacc aggtcctact ccctgtatcc agggtgattc
      181 gagattgcag ggtgcatttg caatctgact ccaggaaaaa gtagtcgtcc aggcgaccag
      241 catgaatgcc gtcagcagcc ggaagaaact cgtcctttga ttcgtgtttt gtttgccatt
      301 tttcatttac acaacaattt ttataattaa atttcaaaaa aaattcggtt attctcatgt
      361 ataacactta caccgtacta aagcattgag cttctgttca atgtaaaaag ttattaattt
      421 ttgttttgcc aggataggta atcaggtttt cagatattgt gacaatatct gaaaggtatt
      481 ttcatttgaa tttattaaat tgtacaagta tctattatac aatataataa acaggactta
      541 aactttttta cgggtatttt tgggatttac gcagataacg ttcatgcaaa gatacaaaaa
      601 ataaaatgag accttgttat tttttcaaaa aactatcctc atctgatact ttgtaatgga
      661 ctcaatgatt aaaaaaaagt aacattctat atcattgaga cggtcaaggg tacaaatatc
      721 cggtaaattt atgactcata aggtacttct gagataaaaa atttattttt gttcaatact
      781 tcttataaat atttcatttc atacaccacc ttatttgaca actcaacaac ccaagcgacc
      841 ttgaaatgcc aatataaaca catcggccag ccgctaaaat atagtgtgat aaataacaat
      901 cttgattaat attattacta taaaattgta tttaatctac tacatctact ataactaata
      961 acaaagatac acatttgata cacaaaataa aaatagtaca agttcgatat tttgaatcaa
     1021 taaaaatcat taatcttaaa tttattaccg ttaactattt acataattat aaattttgaa
     1081 tttaaataat aaatatgtgt ttatttcaaa atattcaatt ctgcaaacga taaaaccggc
     1141 caatattagc ttatttgtcg tatgctgtcg gtaactgctt taagttcgag tgtattgctt
     1201 acatttagtt tttgaaatat ttttgcctta tgagtagaaa cagtagtttt ttgtaatttc
     1261 aaaatagctg aaatttcggt ggttttataa cccttaagga gaaaattcat gatttcgtat
     1321 tctcttggcg taagcaatga caggcggtca attggtattg gtatgccttc atccaagtct
     1381 agcaaagcct tattgatatt ctcaccataa ataactttgc caagttggct tatcaaatca
     1441 atagattttg agcttttacg aatgaatcca tctgcacctt tatccacgaa atgcattgag
     1501 attgcctttt catttgaaaa aataacaact cgaatcaatg gaaaattgag tctaatgagc
     1561 ctaagtactt gagaagtatc tgtaaatagg atattaacat ccatcaaaat gacatcaaag
     1621 tgcttttcgc gcagcttttt aatgcaatca tcgcctgaat cggcataata cactctgcaa
     1681 tctggaaaat tgcggctaca caacataaca attccccagt agatgatttc tgaatcatca
     1741 agcacgagta aagatttctg agttctgtca ggattcatat tcggatataa agtggataaa
     1801 acatccatac ataacagaat caaataatac gccattgata gtcattttgt aaaaaagttg
     1861 acggaaaaac caaaatactc atgtttgtgc ttcaactttt agcataaaat acgattttaa
     1921 atatgaggaa atgatattaa agttcgcact ccttagagta gtaagacaat tcattttaat
     1981 aaaacaataa cctaaaatga taaaagtaaa gagaatctac gaaactgcca catactcgga
     2041 cgggtacagg attttggtgg acagactttg gccgagaaac atgacgaaag agcatgctgc
     2101 tatcggtata tggatgaaag acctagcgcc aagtactgaa ctccggaaat ggtttaacca
     2161 tgaaatggaa aaatggcctg aatttgaaca aaaatataag gcggagctta aagcaaagca
     2221 acaatatctt caacaactcc gtcagctgga aaaagaacac ggcaccataa ccttacttta
     2281 tgctgccaaa gatgaaaagc ataacaatgc acaggtaatc gttgagtggc tacaataaac
     2341 acaagtctaa acgaaaaaag tggctccata ccaaaaaatt tacaagcagg gttttaagat
     2401 taaactttca caatgcaggt caagcttcat tcaatcattt gcattctgca agaagtatat
     2461 tcgttaaaaa aaatttcagt aatatcatta gatagtataa atatattggc caattgtttt
//
LOCUS       DAOQQY010000356         2506 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_22905_length_2506_cov_5.745818, whole genome shotgun sequence.
ACCESSION   DAOQQY010000356 DAOQQY010000000
VERSION     DAOQQY010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22905_length_2506_cov_5.745818"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2449
                     /gene="polA"
                     /locus_tag="PLE23_12345"
     CDS             <1..2449
                     /gene="polA"
                     /locus_tag="PLE23_12345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMX84101.1"
                     /translation="IGTIAKQAARQGYTVFMVTPDKDYGQLVEENIKMYKPGRQGNEV
                     EILGVEEITQNWGIQRPEQVIDILGLMGDSVDNIPGLPGIGEKTASKLIQEFGSVENL
                     IQNTDKLKGKQKEIVENYAAQGMLSKQLATIELNVPIQFDEKEYEISEFDKDKLSELF
                     KELEFRTLAREILGQNETSAGTPGVQADLFGNTVATVAKEEPETKPEYTIASNNISNT
                     THHYTLCDDDALINMLVSKLSMQKSFSFDTETTGIDPNEAELVGMSFSFAAHEGYYVP
                     VPANKSDALKRIEHFRPVLENPNIEKIGQNIKYDFLIMKWHGINIRGPIFDTMIAHYV
                     CEPEMRHKLDYLAEAYLNYKMVPIEDLIGKGVKQLNMRDVDPQQVSDYAAEDADIAWQ
                     LKDEMIALMKKNATSNIYHDIEVPLIQVLAEMEYEGVKIDRQLLYDYSKVLEKQILEK
                     ESEIYKQAGSRFNIASPKQVGEILFDRMKIPYRWKKTASGQYSTDEEKLTELSYEFDF
                     VNTILEHRKLSKLKSTYVDALPNMINPKTGRIHSSFNQARTATGRLSSENPNLQNIPI
                     RDEAGRHIRKAFIPRDENHLLISADYSQIELRLIAEMAGEQAMLEAFQANQDIHAATA
                     AKVYGVPLHEVTSDQRRNAKTVNFSIIYGAGATNLSQQLKIKRAEAKELIDQYFNQYK
                     DLKAYMDNTVEFARKNGYVTTLLGRRRYLRDIDSRNSLARSNAERMAINSPVQGSAAD
                     MIKIAMINIQKALSESNFKTKMILQVHDELVFDVPVSEADKVSELIRNKMIHAIPSLK
                     VPIEVGIGRGQNWLEAH"
BASE COUNT          878 a          643 c          481 g          504 t
ORIGIN      
        1 catcggtacg attgccaagc aggcagccag acaaggatat acagtattca tggtaacacc
       61 ggataaagac tacggccagc tggtagaaga aaacatcaaa atgtacaaac cgggccgcca
      121 aggaaatgag gtagaaatcc tcggcgttga agaaataacc caaaactggg gaatacaacg
      181 accggaacag gttatagaca tcctcggcct gatgggtgac agtgttgaca acattccggg
      241 cttgccgggc ataggcgaaa aaactgcctc caaactcata caagaattcg gctctgtcga
      301 aaacctcata caaaataccg acaaactaaa gggcaaacaa aaggaaatag tagaaaacta
      361 cgccgctcaa ggtatgctgt caaaacaact ggctaccatc gaactcaacg tacccattca
      421 gtttgatgaa aaagaatatg aaatatctga atttgacaaa gataaactct ccgaactatt
      481 caaagaactt gagttcagaa cgctcgctcg cgaaatactc ggccaaaatg aaaccagtgc
      541 cggaactcct ggtgtacagg cagatctgtt cggaaatacg gtagctactg ttgcaaaaga
      601 agaacccgaa acaaaacctg aatataccat cgcctccaac aacataagca ataccacaca
      661 ccactataca ctttgtgatg acgacgcact aataaacatg ctggtaagca agctcagtat
      721 gcaaaaatct ttctctttcg atacagaaac aaccggaata gacccaaatg aagccgaact
      781 cgtgggtatg tcgttttcct ttgcagccca tgaaggctat tacgtacccg ttcctgccaa
      841 taaatcggat gccctaaaac gaattgagca cttcagaccg gtactcgaaa acccgaatat
      901 tgaaaaaata ggccagaaca tcaagtacga tttcctgatt atgaaatggc acggcatcaa
      961 catccgcggt ccgatattcg acaccatgat agcacactac gtctgcgaac ccgaaatgcg
     1021 acacaaactc gactaccttg ccgaagctta tctcaattac aaaatggtac ccatcgaaga
     1081 ccttatcggc aaaggtgtca aacaactgaa catgcgcgat gttgaccctc aacaagtgtc
     1141 tgactacgca gccgaagatg cagatatcgc ttggcaactc aaggatgaaa tgattgccct
     1201 catgaagaaa aatgctacat ccaacatcta ccacgatata gaagtgcccc tcatccaagt
     1261 actcgccgaa atggaatacg aaggtgtaaa aatagaccga cagttgctct acgattattc
     1321 gaaagtgctc gaaaaacaaa tcctggaaaa agaatctgaa atatacaaac aagccggttc
     1381 aagattcaac atagcatcgc caaaacaagt gggtgaaata ttgttcgaca gaatgaagat
     1441 accctaccgc tggaaaaaaa ctgccagcgg ccaatacagc accgatgaag aaaaactcac
     1501 cgaactctcc tacgaattcg atttcgtcaa taccattctc gaacaccgca aactcagcaa
     1561 actgaaatca acctacgtgg atgcactccc caatatgata aaccccaaaa ccggtagaat
     1621 ccacagctca ttcaatcagg ctcgtaccgc cacaggcaga ctaagctccg aaaatcccaa
     1681 cctgcaaaac atacccatcc gcgacgaagc aggcaggcac ataagaaaag cattcatacc
     1741 acgtgacgaa aaccacctgc tcatctctgc cgactactcg cagatagaac tccgcctcat
     1801 cgccgaaatg gccggcgaac aagctatgct cgaagccttc caggctaacc aggacatcca
     1861 cgcagctacc gccgctaagg tctatggcgt tccgcttcat gaagtaacat ccgaccaacg
     1921 ccgcaatgcc aaaaccgtca acttcagtat catctacggc gccggagcca ccaacctctc
     1981 tcaacaactc aaaataaaac gagcagaagc aaaagaactc attgaccaat acttcaacca
     2041 atacaaagac ctcaaagcct acatggacaa taccgtagag ttcgcccgca aaaacggata
     2101 cgtcactact ttacttggca gaagaagata cttgcgcgat atcgattcac gtaattcact
     2161 cgcccgctcc aatgccgaac gaatggctat caactctccc gttcaaggct ccgcagctga
     2221 tatgattaaa atagcaatga tcaacatcca aaaagcattg tctgaaagca atttcaaaac
     2281 aaaaatgata ctccaggttc acgacgaatt ggtcttcgat gtgccggtgt cagaagcaga
     2341 taaggtgtca gaactgattc gcaataaaat gattcatgcc ataccaagtc tgaaagtacc
     2401 cattgaagta ggtatcggcc gtggccaaaa ctggctcgaa gctcattaaa caagtactat
     2461 tttaaaaaaa attgtcgaat aaaaaagatt tatttgtttt tgttag
//
LOCUS       DAOQQY010000357         2462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_23568_length_2462_cov_5.413793, whole genome shotgun sequence.
ACCESSION   DAOQQY010000357 DAOQQY010000000
VERSION     DAOQQY010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23568_length_2462_cov_5.413793"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLE23_12350"
     CDS             complement(<1..882)
                     /locus_tag="PLE23_12350"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84102.1"
                     /translation="MNLRLSFSILVCCLMAACSSEKKVQNNEGPVTDPHSFSMPQHVK
                     AKHLNLDIAVDFESKTISGIATYDLERTSGNEVIFDTRDLTIYEVTLDDKSKTEFTLG
                     QTIAGKEYLGQKLTIKLKPDTRKVSIKYSTSPDAAALQWLSPEQTNDKTWPFLFTQGQ
                     AILTRSWIPIQDSPGIRITYNATVRVPKPLLAVMSATNPTEKNNSGVYTFVMDKPIPA
                     YLMALAVGDLAFAPLGKRTGVYAEQKFLPKCAYEFADVEKILEAAEDLYGPYQWDRYE
                     ILVLPPSFPFGGMENPKL"
     gene            complement(882..>2462)
                     /locus_tag="PLE23_12355"
     CDS             complement(882..>2462)
                     /locus_tag="PLE23_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HMX84103.1"
                     /translation="KQQLPDVIKWVKFSNASWQGNGFYYSRFPEPKGGGELSDANKYH
                     SIYYHKLGTLQSEDKKIFGNDDAPLMTHFCSVTEDERYLAVYAATGTDGFETYFKDLR
                     DPNSKFEKLFSGFDNKSTIVDNNDDFFLVHTNIDAPNYKLIQIDPKFPDKKGWATIIP
                     QSKTDVLESVTTGGGKLFAQYLKNSTNKIIFYDRQGYSPTEIKLPELGTVSGMSGGKD
                     DEDIYYTFSSFLYPPTIFKFNIPSGKAEPYFQTEFKFNPKDYVSKQVFYQGKDGKKIS
                     MFLVHKKGLKSDGQNPCYLYGYGGFNISMVPQYSPSRLLLIENGAVFAMPNIRGGGEY
                     GEDWHKAGMMDKKQTVFNDFIAAAEYLINRKYTSAEKLGIAGGSNGGLLVGACMTQRP
                     DLFAVALPAVGVMDMLRFHKFTIGWAWVPEYGSSEKPEQFAYLYKYSPYHNLKKGTAY
                     PATLVTTGDHDDRVVPAHSFKFAAQLQAFQKGNKPVLIRIEKSAGHGAGKPTTKIIEE
                     QADILSFFLYNTNAVIKY"
BASE COUNT          625 a          510 c          525 g          802 t
ORIGIN      
        1 cagtttagga ttttccattc ctccaaatgg gaatgaaggt ggtaacacaa gtatttcgta
       61 tctatcccat tgataaggtc cgtatagatc ttcagcagct tcaagtattt tctcaacatc
      121 tgcaaattcg taggcgcatt ttggcaggaa tttttgctcg gcatatactc cggtgcgctt
      181 gcctaaaggt gcaaatgcca gatcacctac agccagtgcc atcagatagg caggtattgg
      241 tttatccatt acgaaagtat agactccgct gttgttcttc tctgtcggat tggtagcact
      301 catcactgca agcaatggtt tcggaaccct cacagtggca ttatacgtaa tacgaatgcc
      361 cgggctgtcc tgtatgggta tccaactgcg tgtgagtatc gcttgtcctt gcgtgaataa
      421 aaacggccag gttttatcgt ttgtttgttc gggactgagc cattgtagtg ctgcagcatc
      481 cgggcttgta ctgtatttta tgctcacttt cctggtgtca ggttttagtt tgatggtgag
      541 tttttgaccc aggtattctt tgccggcaat cgtctgccca agtgtaaatt cagttttcga
      601 tttatcgtcg agtgttactt catagatagt aaggtctctg gtatcaaaaa tgacttcatt
      661 gccgcttgtt ctttcaagat cgtatgtagc gatgccggaa atcgttttag attcaaaatc
      721 gactgcgata tcgagattga gatgcttggc cttcacatgc tgtggcattg aaaaggaatg
      781 aggatccgtc accggaccct cattattttg tacttttttt tctgacgaac atgctgccat
      841 tagacagcac accaatattg aaaatgataa tcttaaattc atcaatattt aattaccgca
      901 tttgtgttat aaaggaaaaa agataatata tctgcctgct cttctattat tttggtcgtt
      961 ggtttgccgg cgccgtgccc tgcactcttt tctattctta taagtacggg cttattccct
     1021 ttctggaacg cctgcagctg agcagcgaat ttgaaactgt gtgccggtac aacacggtcg
     1081 tcatggtcgc cagtagtcac caaagtagca gggtaggcag tacctttctt gaggttatga
     1141 taaggcgaat atttgtacag ataagcgaat tgctcaggtt tttcacttga tccgtattct
     1201 ggtacccagg cccaaccgat ggtgaattta tgaaaacgaa gcatatccat cacacctact
     1261 gccggcaatg ctacagcaaa caaatcgggg cgttgtgtca tacatgcacc tacaagcaga
     1321 ccgccattag agccacccgc tataccgagt ttttcagcac tggtatattt tcggttaatg
     1381 agatattcag ctgctgctat gaaatcatta aagaccgtct gctttttatc catcatacca
     1441 gccttgtgcc agtcttcacc atattctcca ccacctctta tgttaggcat cgcaaacact
     1501 gctccatttt ctatcaacaa taatcttgaa gggctgtact gtggtaccat cgaaatgtta
     1561 aatccaccat aaccatatag ataacaaggg ttttggccgt cagactttaa tcctttttta
     1621 tgtaccaaaa acatggatat tttcttgccg tcttttcctt ggtaaaatac ttgttttgac
     1681 acgtaatctt tagggttgaa cttgaattcg gtttggaaat aaggctcagc tttaccactt
     1741 ggtatgttga atttaaaaat tgttggcggg taaaggaatg aagagaaagt gtagtaaatg
     1801 tcttcgtcgt ccttgccccc gctcattccg gatactgtac cgagttcagg gagttttatt
     1861 tcagtaggag agtatccttg acggtcgtaa aaaatgattt tgttggttga atttttgaga
     1921 tattgggcaa acaattttcc gccaccggtg gtcacactct caagtacatc tgtcttagat
     1981 tggggaataa tggtcgccca acctttcttg tcaggaaatt ttggatctat ctgtatcaat
     2041 ttgtaattcg gagcatcaat attggtgtga actaagaaaa agtcgtcatt gttatcaaca
     2101 atggtgctct tgttgtcaaa tccactgaat agtttttcga atttcgaatt cgggtcacga
     2161 agatctttga agtaagtctc gaacccatct gtaccggtag ctgcatatac agccaggtat
     2221 ctttcatctt cggtcactga gcaaaagtgc gtcataagtg gggcgtcatc attgccgaat
     2281 atttttttat cttcggattg tagtgtacct agtttgtggt aataaataga atgatatttg
     2341 ttggcatctg ataattctcc accacctttt ggctcaggga agcggctata gtagaatccg
     2401 tttccctgcc atgatgcatt gctaaatttt acccatttga tgacatctgg cagttgttgt
     2461 tt
//
LOCUS       DAOQQY010000358         2458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_23626_length_2458_cov_4.922597, whole genome shotgun sequence.
ACCESSION   DAOQQY010000358 DAOQQY010000000
VERSION     DAOQQY010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23626_length_2458_cov_4.922597"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..445)
                     /locus_tag="PLE23_12360"
     CDS             complement(41..445)
                     /locus_tag="PLE23_12360"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HMX84104.1"
                     /translation="MACQKKINTANIDTTGATLKNQYWKVTDIDGQKIGKGIEERELH
                     FYLNGSSDIVNGHTGCNSFSGKYRLLGNDKIEFYQLGSTRMYCPEYNGEPLWFDLTDG
                     VLVYEIKRNKLVLTNKKKMRAICETVIQEKQN"
     gene            496..975
                     /locus_tag="PLE23_12365"
     CDS             496..975
                     /locus_tag="PLE23_12365"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HMX84105.1"
                     /translation="MMQVRNSYFSLAFVFLLVVVSNKGCKINQTPQFVNINNVSISDL
                     SLSAIKINSKANFVNPNDIGLTLKNIELSLFVNGKPVGSVNQVKSTKIKARSEFAIPL
                     QINVAQANLLNAVGGLGGAFGAMFGKEFDIEYRGKIKLSKFWIPFTIPINYKTKFKM"
     gene            complement(976..2154)
                     /locus_tag="PLE23_12370"
     CDS             complement(976..2154)
                     /locus_tag="PLE23_12370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HMX84106.1"
                     /translation="MNDKILYARVQTVVGLLSGYSVEEPLSAYLKRHFVQNKKYGSKD
                     RKAIADLMYHYYRLGRMGERLPTEHRIWLAVYLFMADDGRYADIVGEKTGIVETPAEF
                     GGRLEIVERLLGKNRSGLFPLMELLSEKIDPEAFVQSLGSKPEVWIRLRRNANAVRQN
                     LEREAYKLRPSGLLENAFAVENPKGLEQSGMFQKGSFEIQDLSSQLCASYIEAGENAT
                     VWDACAGAGGKALYLADRYPDISIKCTDIRQLIVKNLEARAKRAGQDNIDAEVLDVTD
                     EHRLKNWPGDWKHIVADVPCSGSGTWRRTPEQMAVCTHEQIIKYSELQKTILRNLAAK
                     TAKGGYIYYMTCSLYSVENEDVTSWAVRELNLEITKEETVCGYEYGADTMYFCKLRKL
                     "
     gene            complement(2219..>2458)
                     /locus_tag="PLE23_12375"
     CDS             complement(2219..>2458)
                     /locus_tag="PLE23_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84107.1"
                     /translation="AVQHRVTLPGFFHIRLFFSINMSSLWDFGFLRIFKEIRTIHHSP
                     FTTHHLPFTTYQSRVRDRSRHEVRRIARPDRGTPT"
BASE COUNT          629 a          625 c          466 g          738 t
ORIGIN      
        1 taatgaaact taaggaaaaa taaaaattgt gttacaaaat ttaattctgc ttttcctgta
       61 ttactgtttc gcagattgca cgcattttct ttttattggt caacaccagc ttgtttcttt
      121 ttatttcata caccaacacg ccatctgtca ggtcaaacca cagtggctct ccgttgtact
      181 ccggacaata catccttgta gatccgagct ggtaaaattc tattttatca ttaccaagta
      241 acctgtattt accactaaaa ctgttgcagc cggtgtggcc gtttactatg tccgagctgc
      301 catttaagta aaaatgcaac tcgcgttctt ctataccttt acctatcttt tggccatcta
      361 tgtctgtcac tttccagtac tgattcttca aggtagctcc tgtagtatct atgtttgctg
      421 tatttatttt tttttgacat gccatttggc caaataacaa gataaaaagc agatatttga
      481 gcctaaattt aagtaatgat gcaagtacgg aattcatatt ttagcctggc ttttgttttt
      541 ttgttagttg tagtatccaa taagggttgc aagataaacc aaactcccca atttgtaaat
      601 attaataacg tcagtatttc ggatttgagt ctgagtgcca tcaaaattaa ttctaaagcc
      661 aatttcgtca acccgaatga cattggtctg actctcaaaa acattgagct cagccttttc
      721 gtcaatggca aaccggtagg ttctgtcaac caagtcaaat ctaccaaaat aaaggcacgc
      781 tccgaatttg ccatcccact ccagatcaat gtcgctcagg ccaacctgct caatgcggtc
      841 ggtgggctcg gtggtgcgtt cggcgctatg ttcggcaaag agtttgacat cgaataccgt
      901 ggcaaaatca agctatccaa attctggatc ccatttacca tccccatcaa ttataagacg
      961 aagttcaaga tgtaatcaaa gcttgcgtag cttgcagaag tacatcgtgt ctgcaccgta
     1021 ctcatagcca catacggttt cttcctttgt tatttctaag ttaagctctc gtactgccca
     1081 tgatgtcaca tcttcattct caacagaata tagtgagcat gtcatgtagt atatatagcc
     1141 acccttcgcg gttttggcag ccagattcct aagtatggtt ttctgcagtt cgctgtattt
     1201 tattatttgt tcgtgtgtac agacggccat ctgctcgggt gtacgcctcc acgtaccaga
     1261 gcccgaacac ggcacatccg ccactatgtg cttccagtct cccggccagt tcttcagacg
     1321 gtgttcatcg gtcacatcca gcacttcagc atcaatgttg tcttgcccgg cacgcttcgc
     1381 tctcgcttcc agattcttaa caatcaactg ccttatgtct gtacacttga tgcttatgtc
     1441 agggtaccgg tccgccagat acagcgcctt gccacctgca cctgcacagg catcccacac
     1501 ggtagcattt tctccggctt ctatgtacga agcacacaac tgcgatgaca ggtcctgtat
     1561 ctcaaaactt cccttttgga acatacccga ctgctccaag cctttcggat tttctaccgc
     1621 aaaggcattt tcaagcaatc ctgatggcct aagtttatat gcttcgcgct ccagattctg
     1681 tcttacagca ttcgcattgc gcctcagcct tatccacact tcaggtttcg aacccaggct
     1741 ttgtacaaat gcctctggat ctattttttc agacaacagc tccatcaacg gaaacaaccc
     1801 tgaccgattt ttgcccaaaa gccgttctac tatttcaagt ctgccaccaa actccgccgg
     1861 tgtctccacg atgccggtct tttctcccac tatatcggca taccgaccat catccgccat
     1921 aaaaagatat acagccagcc aaatccgatg ctcggttggt agccgttcac ccattcgtcc
     1981 cagcctatag taatgataca tcaggtcggc tattgctttt cggtctttag aaccatactt
     2041 cttattctgt acgaaatgcc gcttcaggta tgccgacaag ggttcctcaa cactataacc
     2101 actcagcaac ccgaccaccg tctgcacacg cgcgtacaat atcttgtcat tcatgccata
     2161 aaggtatgcc ctttgtacaa cagccagggt gttttcaaca tatttttaat catttttctt
     2221 atgtgggcgt gcccctgtcg ggtcgggcta tccgccttac ttcgtgccgg cttctatccc
     2281 tcacgcggct ttggtaagtg gtgaatggta agtggtgagt ggtgaatggt gaatggtgaa
     2341 tggtgcgaat ttccttaaag atacgcaaaa agccaaaatc ccatagggat gacatattaa
     2401 tagaaaaaaa caatcgaata tggaaaaacc ctggaagggt gacacgatgt tgaaccgc
//
LOCUS       DAOQQY010000359         2456 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_23652_length_2456_cov_5.892128, whole genome shotgun sequence.
ACCESSION   DAOQQY010000359 DAOQQY010000000
VERSION     DAOQQY010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23652_length_2456_cov_5.892128"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1227)
                     /locus_tag="PLE23_12380"
     CDS             complement(<1..1227)
                     /locus_tag="PLE23_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS C-terminal target domain-containing
                     protein"
                     /protein_id="HMX84108.1"
                     /translation="MCWNRILQTSLILVLYTILPSSTYAQVSAITGVSIIPTDIKGIP
                     ALQSFAIGVHEGEWLLIGGRTDGLHQRQPFAAFASEDNNTAISVINPVSGLVWTYDLF
                     TLPSALQEQLQSTNLQFCQQGKQLIITGGYGYSHSRKEHTTFDGLVVVDIPACIEAVK
                     SHKGLSKTIHYIHDSRMAVTGGQLAWLNGYYYLVGGQKFEGSYNPMGPDHGPGFVQEY
                     TNAIRKFKLIKENDSLKIKDYSEIIDTAQLHRRDYNMLPQIFPDGSKGFTVFSGVFQY
                     KADLPWLNTVNISETTYEVVPVFEQKLAQYHCAKTAMFDPIGKTNYNIFFGGISRFYI
                     DENGKLQDDHDVPFVKTISMVIRDSKGQLTEYAIGNLPDYLGASAEFIPSPGYEKIAG
                     TDILSYNPSTADSIFLG"
     gene            complement(1446..>2456)
                     /locus_tag="PLE23_12385"
     CDS             complement(1446..>2456)
                     /locus_tag="PLE23_12385"
                     /inference="COORDINATES: protein motif:HMM:NF027442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HMX84109.1"
                     /translation="IKKYLYGDLPLTPATAYQSSPQARIGFYNDAFLNDWGDQGTFEV
                     KEQCDNPLTTDDYEFLANESKYLPLSGETNGLNPCDGGLRTKGSNALFEMNDSHWSSI
                     NRDYHPAFWNNMDDSVYQEMLRSLGYCFVLIKSTVDVQQDNFNIELEIKNQGYARAFD
                     RRPVFLVLENTENGQTTPIALNTDVRTWSWGTVIGHNISPGVSGTFRLYLWLPDKDSV
                     LSKISDYSIRLANDSLWHPISGYNDLQQTIVLDKKSIVQNPNTSQSNLLYPNPVIEHL
                     SIQTTDEAVHNISITNLVGQLVLQTNIIGADTIDVADLSAGIYFIKMNARYPVVLKFV
                     KL"
BASE COUNT          707 a          510 c          507 g          732 t
ORIGIN      
        1 gcccagaaaa atactgtcag ccgtgcttgg gttataagaa aggatatcgg taccagcaat
       61 tttttcataa ccgggtgaag gaatgaactc tgctgaagca cccaaataat caggtagatt
      121 accgatggcg tattctgtca attggccttt gctatccctt ataaccatgc ttatggtctt
      181 aacgaatggt acatcatgat cgtcttgcag ttttccattt tcatctatgt aaaatctgct
      241 tatgccacca aaaaatatat tgtagttcgt cttccctatt ggatcgaaca tagccgtttt
      301 ggcacaatgg tactgtgcga gtttttgttc gaatacagga acgacctcat aagttgtttc
      361 gctaatgttg acagtgttga gccaaggcaa atctgcttta tactgaaata ctcctgaaaa
      421 aaccgtaaaa cctttagagc cgtcagggaa aatctgagga agcatattgt agtcgcgacg
      481 atggagttgt gcggtatcaa taatctctga gtagtctttt atctttaatg aatcattttc
      541 cttgataagc ttgaactttc taatggcatt tgtgtattcc tgaacgaaac ccggaccatg
      601 atcgggcccc ataggattat aactaccttc aaatttctgc ccacctacca gatagtaata
      661 cccattcaac caagctaatt gcccgcccgt aacagccatt cgtgaatcat gtatgtaatg
      721 gatggtttta ctcagccctt tgtgcgattt aacagcctcg atacatgccg gaatatccac
      781 tactaccaga ccatcaaatg tggtatgttc ttttctactg tgcgaatatc catatccgcc
      841 tgtaataata agttgtttgc cttgctggca aaactgaaga ttagtacttt gaagttgttc
      901 ttgcaatgca ctgggcaatg taaaaagatc gtaagtccag accagccctg agaccgggtt
      961 gataaccgag atggcagtat tgttatcttc agatgcaaat gctgcaaatg gctgacgctg
     1021 gtgaagccca tcggtacggc caccaataag gagccattcg ccttcgtgaa cgcctatggc
     1081 aaaggattgc aaggcaggta tgcccttgat gtcagtaggg ataatgctga cgccagtaat
     1141 agcagacacc tgagcataag tgctagaagg aagaatagtg tacaagacga gtatgagtga
     1201 tgtttgtaaa attctgttcc aacacattac cgaaccagtt ttttgtataa ataaaagaag
     1261 cacaattata caactgtgct cccaaaaatt tattttaatt taaaccgtat ttggctgaag
     1321 atatcatgat tccgaaaaaa cggaacagat gttcagtcaa tacactacaa aattaatata
     1381 atttgtgata actatggtaa ttttgaagct tttatttgaa tattttcaag ataataaatg
     1441 acttcctaca attttacaaa ttttagaacc acaggatacc tggcattcat tttaataaaa
     1501 taaatgccag ccgacagatc tgccacatct atcgtgtctg ccccaatgat gttagtctgc
     1561 aatacaagct gcccgacaag gttggtaatg cttatattat gtactgcctc atcggtagtt
     1621 tggatgctta aatgctctat taccgggttg gggtaaagca ggttgctctg gctggtatta
     1681 ggattttgga caatgctttt tttatcaaga acaatggttt gctgaagatc attatagcca
     1741 ctgattgggt gccacaagct gtcgttcgct aaccggatgc tgtagtcaga aattttgctt
     1801 aacaccgaat ccttgtcggg tagccagaga tagagcctga aagtacctga tactccgggt
     1861 gaaatgttgt gcccaatcac tgtgccccag ctccaggttc tgacatcagt attaagtgca
     1921 ataggtgttg tctggccgtt ttctgtattt tctaacacca gaaaaaccgg tcgtctgtca
     1981 aacgctcttg cgtatccttg attcttgatt tccagctcaa tgttaaaatt atcctgctgg
     2041 acatcaacag ttgatttgat caaaacaaag cagtaaccca agctgcgaag catctcctgg
     2101 taaacactgt cgtccatgtt attccagaag gcaggatgat agtcgcggtt gatacttgac
     2161 caatggctat cattcatttc aaacaaggca ttcgaaccct tggtacgaag accgccatca
     2221 cagggattca agccatttgt ttcgcctgac aagggcaggt actttgattc gtttgccaaa
     2281 aattcataat cgtctgtcgt taatgggtta tcgcattgtt cctttacttc gaaagtaccc
     2341 tgatcacccc aatcattgag aaaagcatcg ttgtaaaaac caatacgtgc ttgtgggctc
     2401 gattggtaag ccgtagccgg tgttaacgga agatcgccat acaagtattt tttgat
//
LOCUS       DAOQQY010000360         2453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_23700_length_2453_cov_5.793995, whole genome shotgun sequence.
ACCESSION   DAOQQY010000360 DAOQQY010000000
VERSION     DAOQQY010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23700_length_2453_cov_5.793995"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="PLE23_12390"
     CDS             <1..1170
                     /locus_tag="PLE23_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84110.1"
                     /translation="DMSNNNALVYSDGTNKVRVDRQALANVLRELHKEEKKIRMVLCD
                     ILLLDTVDAHWGQNPEQLDETQIEVLEGKAARINKSLRDMLDSFQQKDKILIPQMYDS
                     ENRLKIGLAYNEIKTGIAQYKFQFNNSFYRYNYITDGHRGVPIMMWDKLSSGKDTSYT
                     KSFLGLSLLGGNSHRNGGITLNRIIPHFRITQDEIVKDKDYFTASEFRKELIADHSKP
                     LIVVIGNFETRDIHTTIFEKLTGPLLLVNLYEGIRHGDNFFEWWFILLTLGVYIYIGY
                     NSINRLIFKEKHLHFPLPVRQCVRISWGKKHIAWLSNIVSFIFCFSQYILFFLKYHFR
                     EHWVHILLVFYTVFSLIFFNHYLYLLAAVFGLILLNFIIGLVRYFRAMNQVSYKK"
     gene            1314..>2453
                     /locus_tag="PLE23_12395"
     CDS             1314..>2453
                     /locus_tag="PLE23_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HMX84111.1"
                     /translation="MKNEEKKKLFSVSGRPIADNQNVKTAGKRGPMLLEDFWFLEKLA
                     HFDREVIPERRMHAKGSGAFGKFTVTNDITKYTKADIFSKVGKETPAFVRFSTVAGER
                     GAADAERDIRGFAMKFYTEEGNWDLTGNNTPVFFLRDPYRFPDLNKAVKRDPKTNLRC
                     PNHNWDFWTLLPEALHQITITMSERGIPKSYRHMHGFGSHTFSMINADNQRVWVKFHF
                     RTQQGIQNLSNEEATAIIGKDRESHQRDLFNAIEQGDFPKWTMQIQVMTEDQAKKCPF
                     NPFDLTKVWPHGDYPLIDVGVLELNRNPENYFADVEQAAFNPANVVPGIGFSPDRMLQ
                     ARLFSYGDAQRYRLGVNHYQIPVNKPQCPFHNASRDGAMRVDGNEG"
BASE COUNT          758 a          499 c          510 g          686 t
ORIGIN      
        1 gacatgtcaa ataacaatgc acttgtttat tctgatggta caaacaaagt acgggttgac
       61 agacaagcac tggcaaatgt attacgagaa ttgcacaaag aagaaaaaaa gattaggatg
      121 gtgttgtgtg atattttgct tttagacaca gtggatgcac attggggtca aaaccctgaa
      181 caactcgacg aaacccaaat cgaagttctg gaaggaaaag cagcacgtat taacaaatca
      241 ctaagggata tgctggattc ctttcagcaa aaagataaaa tattgattcc acagatgtat
      301 gattctgaga atcgattaaa aataggactt gcctacaacg aaatcaaaac gggcattgct
      361 cagtacaaat ttcagttcaa caattcattc tacagataca attacatcac tgacggtcac
      421 cgtggcgtac ctatcatgat gtgggacaaa ctgagcagtg gaaaagatac atcctatacc
      481 aaatcatttc ttggcctaag tcttttgggt ggtaacagtc acagaaatgg tggtataacg
      541 ctcaacagga ttattcctca ctttcggatt acacaagatg aaattgtcaa ggataaggat
      601 tatttcactg ccagtgagtt tagaaaagag ctcattgcgg atcattcaaa gccattgatt
      661 gtagtaattg gtaattttga aaccagagat atacatacca ctatttttga aaagctaacc
      721 gggccattgt tgttggtaaa tttatacgaa gggataagac atggtgataa tttctttgaa
      781 tggtggttta tactgttgac cttgggtgta tatatttata ttgggtataa tagcataaat
      841 cggcttatat ttaaagaaaa gcacctgcac tttcctttac ctgtacgtca gtgtgtccgt
      901 atctcgtggg ggaaaaaaca tattgcttgg ctttcaaata ttgtttcttt tattttctgt
      961 ttttctcagt atatcctctt ctttttaaaa taccatttcc gtgaacactg ggtacacata
     1021 ctcctggttt tttatactgt gttctccctt attttcttca atcattacct ttacttgttg
     1081 gcagctgtat ttggccttat tctattaaac tttatcattg gtttggtacg ctatttcaga
     1141 gccatgaatc aggtatcgta taagaaataa tagccaaaaa tcggctacat tcggttcggt
     1201 ttgtcatgct caatgcacgc atggttaagt atgattataa tttgtaaccg aaaacataaa
     1261 acatccggca aattgatgta tatttgtgat atcctacaaa taaacatcaa tatatgaaaa
     1321 acgaagaaaa aaagaaactg ttttcagtat ccggaaggcc gatagctgat aaccaaaacg
     1381 taaaaacagc cggcaaacga gggccaatgt tgctcgagga tttctggttt cttgaaaaac
     1441 ttgcccactt cgatcgcgaa gtcattcctg aaaggcgtat gcacgcaaaa ggctcaggtg
     1501 cattcggtaa atttacggtc acaaacgata ttaccaaata caccaaagct gatatcttct
     1561 caaaagttgg gaaggaaaca cctgcatttg tccgtttctc aactgtggcc ggcgaacgtg
     1621 gtgctgctga tgcagaacgc gatatccgtg ggttcgctat gaagttctat accgaagaag
     1681 gtaactggga cctcaccggt aataatacgc cggtgttttt cttacgcgat ccgtaccgtt
     1741 ttcccgatct caataaggct gtaaaacgcg acccgaaaac aaacctgcga tgccctaacc
     1801 ataactggga tttctggaca ctcctgccgg aagcactgca tcagataacc attaccatga
     1861 gtgagcgagg tattccaaaa tcttacagac acatgcacgg attcggaagc cacactttca
     1921 gtatgatcaa tgctgacaac caacgggtat gggtcaaatt tcacttcaga acacaacaag
     1981 gtatccagaa tctttcaaat gaagaggcta ctgccatcat aggtaaagat cgcgaaagcc
     2041 accaacgaga tctgttcaat gccatagagc aaggcgattt tccaaaatgg accatgcaga
     2101 tacaggtgat gaccgaagat caggcgaaaa aatgcccttt taatccgttt gaccttacta
     2161 aggtctggcc tcatggcgac tatccgctta tagatgtagg tgtacttgag ctgaacagaa
     2221 atccggagaa ctacttcgcc gatgtcgagc aagctgcctt caatcctgct aatgtagtac
     2281 cgggtatcgg cttttctcct gacagaatgt tgcaggcaag attattctct tacggcgatg
     2341 cccagcgtta ccgcctcggt gtaaaccatt accagatacc ggtcaataaa ccacaatgtc
     2401 cgttccataa tgcttcgcgt gacggggcaa tgcgtgtgga cggcaatgag ggt
//
LOCUS       DAOQQY010000361         2450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_23748_length_2450_cov_4.810021, whole genome shotgun sequence.
ACCESSION   DAOQQY010000361 DAOQQY010000000
VERSION     DAOQQY010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23748_length_2450_cov_4.810021"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PLE23_12400"
     CDS             <1..1008
                     /locus_tag="PLE23_12400"
                     /inference="COORDINATES: protein motif:HMM:NF027510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HMX84112.1"
                     /translation="ISGSGTPTVTSKITILGNGAISDVSISKIKGFHEYFNDLDVTLQ
                     SPAGTKNKLFTDQCKDFAVSFDFGLDDNASKNFTCPPTLGNVFKPLEPFSIFKGEESA
                     GEWTLIVKDLHPSGGGELSEWNLKVCTGVSLNPPYIITNDTLKLKPHTGKKIDLLHLE
                     TGDANNTPEQLSYTLVKLPEHGLVGNGNQQLFVGNKFTQQDINDAKFSYFNTEDNDID
                     DNFYFTVEDGEGGWIGIIKFNIRTDENILISTKDVAANQVLIYPNPAKDEWRISLTDN
                     NTIVYRIDVYNLQGMKVRTYNQDETTNNRLSTQNLITGTYFVNIITSTGIFTQKLVIQ
                     K"
     gene            1119..>2450
                     /locus_tag="PLE23_12405"
     CDS             1119..>2450
                     /locus_tag="PLE23_12405"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMX84113.1"
                     /translation="MKTGIFFGGPSREREISFAGGRTVYDNLNKSIFQPIPIFVDSFR
                     NLILLDWQYIYKGTIRDFCPPVALIPESDHDFQIYEESIAYQNQEMQEEIIRKLGRKI
                     EISELPALIDFAFLTLHGSFGEDGQIQGLLDSIGIPYTGSGLRACSIGMDKAFQKKLM
                     STAGFACPEVIVIQKEEWLQTDFNSFYPEIIRKIGYPVVVRPANQGSSIGVSIIQEVQ
                     GKEAVYNAIEKAFFRRSIRLSDWITLNKTEKIQYIRELIDIRDGLGFPVDAEGITILH
                     PDELLNFIENQALVNPEKTLVLNSHLSEQKVILEQFIEGREFSCIVLRKENGEVVALP
                     PTEIVKGGELFDYKSKYLPGLSRKLTPINLLESDIGSIRTECERLYRFFEFGTYARID
                     GFFTPTKQIFLNDPNTTSGMMPSSFFFHQAAEIGLNPSEFLTLIIRTSIQER"
BASE COUNT          807 a          503 c          473 g          667 t
ORIGIN      
        1 atatcgggta gtggcacacc tactgtcaca tctaaaataa ccatcctggg caatggagcc
       61 atcagcgatg tgagtatttc aaagattaaa ggttttcacg aatattttaa cgaccttgat
      121 gtgaccttac aaagccctgc cggcacgaag aacaaattat ttaccgatca gtgtaaagat
      181 tttgctgttt cttttgattt cggtctcgat gataatgctt ccaagaattt tacctgcccg
      241 ccgactttag gtaatgtatt taagccactt gaaccttttt caatatttaa gggagaagaa
      301 agtgcaggcg aatggacact tatagtcaaa gaccttcacc cgtcaggtgg tggtgagctt
      361 tcagagtgga acctcaaggt gtgtacaggt gtaagtctca atccaccata catcatcacc
      421 aatgatacgc taaagcttaa acctcacacc ggtaagaaaa tcgacctgct acaccttgaa
      481 acaggcgatg ccaacaatac acctgagcaa ttgagctata cattggtcaa actacccgaa
      541 catggcttag tgggtaacgg aaaccagcag ttgtttgtag ggaataaatt tacccaacaa
      601 gacatcaacg atgccaaatt ttcgtatttt aatactgaag ataatgacat cgacgacaat
      661 ttttacttta ccgtagaaga tggagaagga ggctggatcg gtatcatcaa attcaacatc
      721 cgaacagatg aaaacatcct cataagtaca aaagatgtcg cagcaaatca agtacttatc
      781 tatccgaatc ctgcaaaaga cgaatggcgc atttcactta cagacaataa taccatagtg
      841 tatagaatag atgtgtacaa ccttcaaggc atgaaggtgc gtacatacaa ccaggatgaa
      901 acaaccaaca acagactgag cactcaaaac ctcattacag gtacatattt tgtaaatatt
      961 ataacttcaa ccggtatttt cacacaaaaa ttagttattc aaaaatagat taccagcatt
     1021 tagcatacaa cctgcccgtt atacattaac aggcaggttt ttttatttaa tacagaaatc
     1081 ttacctttac acttattatc aaaattataa ctgctttgat gaaaacaggc atatttttcg
     1141 gagggccatc cagagaaaga gaaatatctt ttgccggagg tcgaacagta tatgacaacc
     1201 ttaataaatc tatctttcaa cctataccaa tctttgttga ttcattcaga aatctgattc
     1261 ttcttgactg gcagtatata tacaaaggca ccatacgcga cttttgtcca ccagtagcat
     1321 tgattcctga atcggaccat gattttcaga tttacgaaga aagcatagca taccagaatc
     1381 aggaaatgca ggaagaaata atacgcaagc ttggtaggaa aatagagata tccgagctac
     1441 ctgcattaat tgattttgca tttcttacac ttcatggcag ttttggtgag gacggtcaga
     1501 tccagggttt gctggacagc atcggtattc cttataccgg ctcaggtctg agagcctgct
     1561 caatcggtat ggataaggca ttccagaaaa agcttatgag tactgccggt tttgcctgtc
     1621 cggaggtgat tgtgattcaa aaagaagaat ggttacaaac tgatttcaat tcattttatc
     1681 ctgaaataat ccgaaaaatt ggttatccag tggtcgtacg acctgccaac caaggttctt
     1741 caatcggggt tagcattatt caggaagttc aaggcaaaga agccgtttac aacgcaatag
     1801 aaaaagcatt tttcagaaga agtatccggc taagcgattg gattacattg aataaaacag
     1861 aaaaaataca atacatccgt gaattaatag acatacgcga cggacttggt tttccagttg
     1921 atgcggaagg gataaccata ctccatccgg atgaattatt aaattttatt gaaaatcaag
     1981 ccttagtaaa tcctgaaaag actttagtac ttaacagcca cctaagcgaa caaaaagtaa
     2041 ttctcgagca attcatcgaa ggacgtgaat tctcctgcat cgtacttcgc aaggaaaatg
     2101 gtgaagtagt agcattacca cctactgaaa tagtcaaagg cggagaatta tttgattaca
     2161 agtcaaaata tcttcccggc ctgtcaagaa agctaacgcc gataaacctc ttggagtctg
     2221 acattggatc tataagaaca gaatgcgagc gcctgtatcg tttttttgag tttggtacgt
     2281 atgccaggat tgacggattt tttacgccga caaaacaaat ttttctcaac gacccgaata
     2341 ccacttccgg gatgatgccg tcttcttttt tcttccacca ggcagcggag ataggcctta
     2401 acccaagcga atttctcaca ctcatcatac gaacgtctat ccaggaacgc
//
LOCUS       DAOQQY010000362         2449 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_23769_length_2449_cov_4.544695, whole genome shotgun sequence.
ACCESSION   DAOQQY010000362 DAOQQY010000000
VERSION     DAOQQY010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23769_length_2449_cov_4.544695"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="PLE23_12410"
     CDS             complement(<1..1473)
                     /locus_tag="PLE23_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HMX84114.1"
                     /translation="MNRKFSPKVKQVITNSRNEAVRLGHDYIGTEHFLLGILQEKTCL
                     ALKVLDSLELDKNQLKSTIEDALPKRSNQTTAINIGNIPLNKQAERVLKITFLEAKNL
                     KSDEILPEHLILSILKNKDNEASRILHRFDVDYEIYKSELEFVKQDELNPGPAIFNET
                     PSDQDEPFEDDDEGASRMQRKSGSKSRTPVLDNFGRDVTKLAEEGKLDPIIGRENEIE
                     RVSQILSRRKKNNPILIGEPGVGKTAIVEGLALRIMQKKVSRTLFNKRIIMLDLAALV
                     AGTKYRGQFEERMKAIMNELEKSRDVILFIDEIHTIVGAGGATGSLDASNIFKPALSR
                     GELQCIGASTLDEYRQHIEKDGALDRRFQKVIVDPPSPEEAVHILKNIRPKYEEFHNV
                     TYSDDVIKSAVMLSTRYISDRFLPDKAIDIIDEVGARVHLKNIHVPKHIEELEQKIED
                     IKEQKNLAVRSQQYEKAADLRDDESKMIRQLEFAKIEWEEE"
     gene            1686..2066
                     /locus_tag="PLE23_12415"
     CDS             1686..2066
                     /locus_tag="PLE23_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMX84115.1"
                     /translation="MKFEVEKEEKYTIFRLNENNLNSIIAPMLKSEFVMLNSEGVKNL
                     IFDMSEIHFVDSSGLSAILTANRLWKDNGLFVMTGITHPNVIKLIDISRLNSVLTIIP
                     TVEESVEYVFMDEIEKELYSESDD"
     gene            2073..>2449
                     /locus_tag="PLE23_12420"
     CDS             2073..>2449
                     /locus_tag="PLE23_12420"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX84116.1"
                     /translation="MNFEVCILGCNGAVPQKGKYPSCQYVRIDTFHCLVDCGEGAQFQ
                     MVNLGVPKHRIEHIFISHLHGDHIFGLIGLITSYLLAGRTKALDIFSPQGLEEIIKIQ
                     LQANGKFEMPFELRFHVIDTTTPG"
BASE COUNT          639 a          473 c          488 g          849 t
ORIGIN      
        1 ttcctcttcc cattctattt tggcaaattc gagttggcga atcatttttg attcgtcgtc
       61 gcgcaagtct gctgcctttt cgtactgttg acttctgact gccaaattct tttgttcctt
      121 gatgtcttct attttttgct ctagctcttc aatgtgcttt ggaacatgta tattcttcaa
      181 atgcactctg gcacctactt cgtcaataat gtcaatggct ttgtcgggta aaaatcggtc
      241 gctgatatag cgtgtgctaa gcataacagc cgatttgata acatcatccg aataggtcac
      301 attgtgaaat tcttcgtatt ttggacgaat attttttaga atatgtactg cttcttccgg
      361 cgaaggcgga tctacaatta ccttttgaaa tctcctgtcg agtgcaccat ccttttctat
      421 gtgctgtcga tattcatcaa gtgtagatgc acctatacac tggagttcac cacgtgacag
      481 tgcaggtttg aaaatattgg atgcatcaag cgacccggta gctccgccag ctcctacaat
      541 ggtatgtatt tcatcaatga agagtatgac atctcttgat ttctcaagtt cgttcatgat
      601 ggccttcatt ctttcttcga attgtccgcg atattttgtg ccagctacaa gtgcggcaag
      661 gtcaagcatg ataatccgtt tgttgaacaa tgtgcgggaa acttttttct gcataatgcg
      721 caatgccaaa ccttcgacaa tggctgtttt accaacgcct ggttcgccta tgagtatcgg
      781 gttgttcttc tttctgcgac ttaatatctg cgatacgcgt tctatttcgt tttcgcggcc
      841 tatgataggg tcaagtttgc cttcttcggc cagtttggtc acatcgcgcc cgaaattgtc
      901 aagtaccggt gtacggcttt tcgagccact tttgcgttgc attctggaag caccttcatc
      961 atcatcttcg aatggttcat cttggtctga gggtgtttcg ttgaatattg cagggccggg
     1021 atttaattca tcttgtttta caaattcgag ctccgactta tagatttcat aatctacatc
     1081 aaacctgtga agtatgcgtg atgcttcgtt gtctttgttt ttcaaaatag acaggatgag
     1141 gtgttctgga agtatttcgt cacttttaag gttctttgcc tcaaggaaag ttatcttcag
     1201 tacccgttct gcctgtttgt taagtggaat attgccgatg ttgatagccg tggtctggtt
     1261 cgaacgctta ggcagtgcat cttcaatggt tgattttaac tgattcttgt cgagttcgag
     1321 tgaatcaagt actttcaggg ctaagcaggt tttttcctga agtatcccta gaagaaaatg
     1381 ctctgtacca atataatcgt gccctaagcg gacagcttca tttcggctat tagtaatgac
     1441 ttgttttacc ttcggagaaa attttctgtt cattctttta ttttatggtc gcaattttaa
     1501 gacaagtata atgctattct tattagaaaa caaaattttt ttactgttta tgaataccaa
     1561 atacatgcca tagcttaata acgtgaaata ttgtcatgtg ttcattctat ttacatacat
     1621 tttttttgat attatttaaa aaactcttta tacctttgac aattgaaccg tagtagaaat
     1681 caattatgaa gtttgaagta gaaaaagaag aaaaatacac gattttcagg cttaatgaga
     1741 acaatctgaa ctccattata gctccaatgt tgaaatctga attcgtaatg cttaattctg
     1801 aaggtgttaa aaatcttatt tttgatatgt cagaaataca tttcgttgat tcatcaggtc
     1861 tgagtgccat tttgactgcc aataggctgt ggaaagataa cggactgttc gttatgacag
     1921 gtataacaca ccctaacgtg ataaaactga tcgatatctc gcgcctcaac agcgttttga
     1981 ctatcattcc tacagtcgaa gagtcggttg agtatgtttt tatggatgaa attgaaaaag
     2041 aactttattc tgaatccgac gattaatctg ttttgaattt tgaggtttgt attcttggat
     2101 gtaatggtgc ggttccacaa aaagggaagt acccgagttg tcagtatgtc cgcatagata
     2161 cttttcactg ccttgtagat tgcggcgaag gtgcacaatt tcaaatggtt aatctaggtg
     2221 tacccaaaca ccggattgaa catattttta tttcacatct gcacggcgac catatttttg
     2281 ggctcatagg gcttattaca agttatcttc ttgcaggacg taccaaagca ctcgatattt
     2341 tttcaccgca aggtcttgaa gaaataataa agattcagct acaggcaaac ggaaaatttg
     2401 aaatgccatt cgagcttagg tttcatgtca ttgatacaac tactccagg
//
LOCUS       DAOQQY010000363         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_23876_length_2443_cov_5.270101, whole genome shotgun sequence.
ACCESSION   DAOQQY010000363 DAOQQY010000000
VERSION     DAOQQY010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23876_length_2443_cov_5.270101"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLE23_12425"
     CDS             complement(<1..375)
                     /locus_tag="PLE23_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMX84117.1"
                     /translation="MEFNKQETKEKLKKWTLRILLGYLALSFIWVLTYRWVNPPGTFL
                     MLSRKFSSENAKIRYDWVDYENISSYLKVCAVASEDQNFSDHTGVDFDAIEKAVEYNK
                     NHTKKRGASTITQQVAKNVFLWP"
     gene            466..1878
                     /locus_tag="PLE23_12430"
     CDS             466..1878
                     /locus_tag="PLE23_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HMX84118.1"
                     /translation="MRLILFLATLFFFVDEVKSTNIVDVQIDVAGYKKDTIIFGSYFG
                     GERKEIHPVIRSNDGKFHITSGKLFPEGMYYVQFLPNGDYFEFIFDSDQQFVITTTYP
                     GILDKIKCSGSAENELFFQCVSIINVNRSDADKKIKMIGLLQFRDPERAKELQRELDM
                     LNETVVQQQSYFTQRYPNSISHKYVVASSLPVPDEYNFQDTIERKKADQYVRTHYFDN
                     IDFNDGQLLRSPYPYNMIQSYLNMNLDSPDQLIAAVDHIMKFIMRANDNKKVYFAFLA
                     RLLSSDRPKILEYAHVHLLLSYVQGGKTPWISKADSTQLVSLAIQKEKLLVGKPSPDL
                     IFETKDGTKKSILANDARLTVLYFGDYNCDACQEGFSGLLEFSDYFKDKNVKVVGICT
                     NGGDEYKKCFKYAEAFHVSFDFLADPVNGRKAKEIFNLPDTPVLFLLDKDKTILAKRL
                     ALPELYKIVSKELEKDKSNE"
BASE COUNT          798 a          413 c          422 g          810 t
ORIGIN      
        1 aggccagagg aacacatttt tcgctacctg ctgcgttatg gtactggcac cccttttttt
       61 cgtgtgattt ttgttgtatt ccacagcttt ttctatggca tcaaaatcaa cacctgtgtg
      121 gtcagaaaaa ttctggtctt cacttgcaac agcacacacc tttagatatg atgagatatt
      181 ttcatagtca acccaatcat aacggatctt ggcattctcg gatgaaaact tacgggaaag
      241 cataaggaat gtaccaggcg gattgaccca acggtaggtc agaacccaga tgaatgacaa
      301 agcaagatac ccaagtagga tgcgcaacgt ccattttttt agtttctctt ttgtttcctg
      361 tttgttaaat tccatttaat tattcagcaa tgtttcttaa gattgataaa agactataat
      421 tttatgcact tttttatttt ctataaaact cagtaaaaat actatatgcg tttgatatta
      481 ttcctggcta cacttttctt ttttgttgac gaagtaaaat cgaccaacat tgtcgatgta
      541 cagattgatg tcgcaggata taaaaaagac accattatct ttggtagtta ttttggtgga
      601 gagcgtaaag aaattcaccc ggtcattagg tcaaatgacg gaaaatttca tatcacaagt
      661 gggaaattat tccccgaagg tatgtattat gtacagtttt tgcccaatgg cgattacttt
      721 gagtttatct tcgattctga tcaacaattt gttatcacta ccacatatcc aggtattctt
      781 gataagatta aatgtagcgg ctctgctgag aatgaattgt tttttcaatg tgtaagtatc
      841 ataaatgtca atagaagtga tgccgataag aagataaaaa tgattggcct tctgcaattt
      901 cgcgatcccg aaagggccaa agaactacaa agagaactcg atatgctcaa tgaaacggtg
      961 gttcagcaac aatcatattt tacacaacga tacccgaact caatatcgca caagtatgta
     1021 gtagcctcaa gtttgcccgt acccgatgag tataattttc aggataccat tgaaagaaaa
     1081 aaagctgatc aatacgtacg aactcattat ttcgataata tcgacttcaa tgatggccaa
     1141 ttgctcagat cgccttaccc ttacaatatg attcaaagtt acctcaatat gaacctggat
     1201 agccctgacc aattaattgc agctgttgat catataatga aatttatcat gcgtgccaat
     1261 gataataaaa aggtctattt tgccttcctt gccagactac tttcgtctga tagacccaaa
     1321 atactcgagt atgcacatgt tcatttattg ctcagttatg tacagggtgg taaaacacct
     1381 tggatttcaa aagcagattc tacacagctt gtttcattgg ctatccaaaa ggaaaaacta
     1441 ttagttggca aaccgtcacc cgacttgata tttgaaacca aagatggcac caaaaaaagc
     1501 attctggcca atgatgcacg tcttacagtc ctgtattttg gcgattataa ctgcgatgct
     1561 tgtcaggagg gattttcagg tctgcttgaa ttttctgact attttaagga taaaaatgta
     1621 aaagttgttg gcatctgtac caacggtggc gatgagtata aaaaatgttt caaatatgca
     1681 gaagcatttc atgttagttt cgattttctc gctgatccgg tcaatggtag gaaagcaaaa
     1741 gaaatattca atctgccgga tactcctgtg cttttcttac tggataaaga caaaactatt
     1801 ttggcaaaaa ggcttgcctt gcccgaattg tataaaatag taagcaaaga actcgaaaaa
     1861 gataagtcaa atgagtaaaa aatatttgac tttctatttt ttctaaaaat aggaggtcaa
     1921 tacaccgtaa taaagtcaca actatacaat ttttcatttg gtaaataaaa taggattctt
     1981 attaaggaat tttttatttg acagttgcaa ataaaggtct gattcaccgt attggtaata
     2041 atatagatat gtattctatt gattgatata tatatacgtt aatatatttt gtacttatta
     2101 ccttgttcaa taaattggtg cgatcatatt cgttaagtcg aagcatattg tttcctacat
     2161 ggatattagt ttcgtaaagc catttcaaag gaaatataat gtcacaattc atcaataaaa
     2221 caaactgtag gctacagatg atattgtgac atttcaattt ttttttcact tgttttgcaa
     2281 atttttcatt tttattattg ccgtgtaaat caatggtata taatttgcat tgtaaaataa
     2341 tataaaaaaa tgaatgtgtt tagatatttt tattcatgac atttttttga aaacccaaat
     2401 tattcattta aatttgagca attaaaatat gatgaaaact ccg
//
LOCUS       DAOQQY010000364         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_24049_length_2433_cov_7.456266, whole genome shotgun sequence.
ACCESSION   DAOQQY010000364 DAOQQY010000000
VERSION     DAOQQY010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24049_length_2433_cov_7.456266"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(609..1316)
                     /locus_tag="PLE23_12435"
     CDS             complement(609..1316)
                     /locus_tag="PLE23_12435"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMUG2 DNA glycosylase family protein"
                     /protein_id="HMX84119.1"
                     /translation="MTSKSPSIAAAVIAFNQNLQYYGALPAGFRVLNPFRDNPETMLV
                     MEQFYMKYYADNKQRRFIIGINPSRHGAGVTGVPFTDTKRLSAVCGIQMTSVHTHEVS
                     AVFMYNMIAMYGGAERFFGDFYINSPFPLAIVKEAKSGALLNANYYDDKELFECVKDF
                     MVETLRAHIALGLDKDEVFVLGKKNAQFISRLNQEAQLFGRLTILEHPRFIQQYKLRE
                     QSSYIDKYILAFSQAKT"
     gene            1600..1731
                     /locus_tag="PLE23_12440"
     CDS             1600..1731
                     /locus_tag="PLE23_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84120.1"
                     /translation="MPNKGQLYLILHTNAYVSAASRLKWRNKYVHTPTVNAARSGSE"
     gene            1875..>2433
                     /locus_tag="PLE23_12445"
     CDS             1875..>2433
                     /locus_tag="PLE23_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMX84121.1"
                     /translation="MRKQFVIISTLLLLAVLGLSLFLNKNWWVFFAVILVLTIMGYQD
                     MFQRQHAIRRIYPLFGRLRYVLEELRPKMYQYFIESDIDGRPINRVDRSTIYQRAKKE
                     LETIPFGTQLDVYAEGYEWMCHSVSPKAFDTLNQDPRVMFGNKDCKQPYSASIFNISA
                     MSYGSLSSQAVEAMNGGAKIGNFAHN"
BASE COUNT          685 a          523 c          514 g          711 t
ORIGIN      
        1 ttggtactta catgtgtgta aatttcggtg gttttacggc tgttgtgacc caataattct
       61 ggcatcgccg gctactattt tgtaaataat taattttttt atgatacaat attattggtc
      121 aaacttaagg tttaatacat tcataacgtg ctgattatgc tgataaaagg agtatttagc
      181 gtaatattac gattatagaa aaaattaatc gtaagtttac agtaaaagtg tgattactgc
      241 tgtgtcaagt aggcttgtct tcgagataat ctactttggc ccaaaagctc aaaaaaacct
      301 agttcactgc gcttctactc cttttttatc gatcttttgt gctcatcact tttttcaaaa
      361 ttcctattgc gttatccggg tattataaat ctactgctta atggttcgaa aaatgccgaa
      421 aaataatgtg tcacaacatc tcacatttta cacaccattc accacttacc acttaccatt
      481 caccatagtt gcgtgaagga cagaagcgaa cacgaagtaa agcggatggc ctgacccgac
      541 aggggcacgc cctaatccat ttatttatac atggtatgat aatagactat ctgtctgcat
      601 cttgtacact atgtctttgc ctgactgaat gccaggatgt atttgtcaat gtagctcgat
      661 tgttctcgca atttgtattg ttgtatgaac cgcggatgct cgagtatagt taagcggccg
      721 aagagttgtg cctcttggtt gagcctgctg atgaattggg cgtttttctt gccgagtacg
      781 aacacttcgt ctttatcaag ccctaatgcg atgtgcgcgc ggagcgtttc gaccataaaa
      841 tccttgacac actcgaataa ttccttgtcg tcgtaatagt tggcattgag cagggcaccg
      901 gatttggcct ctttgacgat cgcgagcgga aaaggcgaat tgatgtaaaa atctccgaag
      961 aaacgttctg caccgccgta catggcaatc atattgtaca tgaacacggc cgaaacctcg
     1021 tgtgtgtgca cggatgtcat ctgtatgcca cacactgctg acagccgctt ggtatcggtg
     1081 aacggcacgc ctgtgacacc ggctccgtga cggcttgggt tgatgccgat gatgaaccgc
     1141 cgctgcttgt tgtcggcata atatttcata taaaactgct ccatcaccag catcgtttcg
     1201 gggttgtccc tgaatggatt aagcactcga aaacctgccg gtaatgctcc gtagtattgc
     1261 aggttttgat tgaatgctat cacagctgct gctatcgaag ggctcttgga tgtcatattg
     1321 tgcaattgtg gtttgtggtc acaatgtacg cctgcagctt gtaacagcca aacctgtaca
     1381 tcaaaaaaac tgacaagaat ggtgtggcgt acagcaggct atctattgga taaattaaaa
     1441 aatccgtggc gtaactatga ctcaccatcc ggataaatat attagtacat acgatggcag
     1501 caatgtttta agccttagtc ctgagaatat ttcatttgtt attgaaccaa ttgggcagat
     1561 acttgttgtt gtctgcatct atcaaacgca taacaagcca tgccgaataa aggccagttg
     1621 tatttgatcc tacacacaaa cgcctacgtc agcgctgcca gccgactgaa gtggcgcaat
     1681 aaatacgtac atactccgac agtgaatgca gctcgttcag gcagcgagta aaatcatttt
     1741 cctgtcctta gtcttcacga aaacatatta attaatagta cctttgtact tgtttaatgt
     1801 gtttgcagga aaaaatttca ggaaaaacct ataaaaacat tataactcaa catcataaaa
     1861 aatcaaaatt cgacatgcga aaacaatttg taatcatctc tacattgctg ttgttggcgg
     1921 ttctcggtct gtcactcttc ctcaacaaaa actggtgggt cttttttgcc gttatactgg
     1981 tactcaccat tatgggatac caggatatgt tccagaggca gcatgctata cgcagaatat
     2041 atcctctgtt tggcaggcta aggtatgtat tggaagaatt aagaccaaag atgtaccaat
     2101 actttataga gtcggacatc gacggtcgcc ccataaaccg ggttgaccga tctaccatct
     2161 accagcgtgc caaaaaagaa ctcgagacca tcccttttgg cacacaactc gatgtatatg
     2221 ctgaaggcta cgagtggatg tgtcattcag tatcgcccaa agcattcgat accctcaatc
     2281 aagacccacg ggtgatgttt ggcaacaaag actgcaaaca accctattcg gccagtatat
     2341 tcaatatttc ggccatgagc tatggttcgc ttagctcgca ggctgtggag gcaatgaacg
     2401 gaggtgctaa gattggcaat tttgcacaca ata
//
LOCUS       DAOQQY010000365         2433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_24051_length_2433_cov_6.125315, whole genome shotgun sequence.
ACCESSION   DAOQQY010000365 DAOQQY010000000
VERSION     DAOQQY010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24051_length_2433_cov_6.125315"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1024)
                     /gene="moeB"
                     /locus_tag="PLE23_12450"
     CDS             complement(<1..1024)
                     /gene="moeB"
                     /locus_tag="PLE23_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HMX84122.1"
                     /translation="MATIVIPTPLRKFTNQQSKVLVNGSTVKESIDNLIQDFPDLKKN
                     LLEDNGNIRGYVNIFVGEDDIRDLQKEQTSVSEHSTISIIPAIAGGCGADDISFNKEE
                     FARYNRHIIIPDFGLEAQKKLKASKVLIIGSGGLGSPSLLYLVAAGVGTIGIVDFDVV
                     DDSNLHRQVLFGVEDVGKPKVEAAKKRLLSLNPHINIITYNTQLTSQNALDIIKDYDV
                     VADGTDNFPTRYLVNDASVLAGIPNIYASISQFEGQVSVFNYTDKNGVTGPNYRDLYQ
                     TPPEPGLVPNCAEGGVLGVLCGIIGTLQANEVIKVVTGVGEPLSGRFFVFDALSFETR
                     ILKISKQ"
     gene            complement(1050..1484)
                     /locus_tag="PLE23_12455"
     CDS             complement(1050..1484)
                     /locus_tag="PLE23_12455"
                     /inference="COORDINATES: protein motif:HMM:NF025819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HMX84123.1"
                     /translation="MLHISRKTEIEILADALRSFPDECCGFMFGTENGEERTISEIQI
                     VNNAKVGDKRRRFEITPFDYMKAEQYAEANNLLLLGVYHSHPNHPSVPSEHDRVVAQP
                     YFSYLIIAVRENEFVSLQSWRLNDDFQFDEEQIAREAEISIK"
     gene            complement(1489..2430)
                     /locus_tag="PLE23_12460"
     CDS             complement(1489..2430)
                     /locus_tag="PLE23_12460"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HMX84124.1"
                     /translation="MSNRVSYKQQTGSHGLKHKLDHISRFIGNTPLLPITGLHQNKDV
                     KIFAKAEWKNLSGSVKARAAFNIIKNAISSGKLTENKILLDATSGNTGIAYAAIGQKL
                     GIKVALCLPENASQERKDILHSLGAEIINTSPFGGTDEAQEKAAELAKDFPKKYFYAS
                     QYTNDNNWKAHYYGTAIEIIRELPEISHFVAGLGTTGTFVGTSRRLKEYNPAIQAISL
                     QPDVAIHGLEGWKHLETAIVPEIYDAQIADYGLEIDTFKAYDLMRKKVLGGVLSPSSA
                     ANIVGAATVADNIESGVIITILPDNADKYSEVIKKIM"
BASE COUNT          603 a          543 c          506 g          781 t
ORIGIN      
        1 tttgttttga aatttttaaa atgcgcgtct caaaactcaa ggcatcaaat acaaagaatc
       61 ttccgctcag tggctcgcca actcctgtaa ctacctttat aacttcattc gcctgtagtg
      121 tcccgatgat gccacacagt actccgagta caccaccttc ggcacagttc ggcaccaggc
      181 ccggttcagg tggcgtttgg taaagatcgc gataatttgg gccggtcaca ccgtttttgt
      241 cggtataatt aaaaaccgaa acctgacctt caaactggga aatggatgca tagatattcg
      301 ggataccggc aagcacactc gcatcattta ccagatatcg tgtcgggaaa ttgtcggttc
      361 cgtctgctac cacgtcgtag tctttgatga tatccaaggc gttttgcgat gtgagctgag
      421 tattgtaggt aatgatattg atatgcggat tcaggcttaa aagtcgtttt ttcgctgctt
      481 ctacttttgg ttttccgaca tcttctactc cgaacagcac ctgacgatga agattactat
      541 catcaaccac atcaaagtca acaataccga tagtgcccac accggccgcc acaagataaa
      601 gaagtgacgg gctgcccaag ccacctgaac caataattag tactttcgaa gctttgagct
      661 ttttctgtgc ctccagacca aaatcgggaa tgatgatatg ccggttgtat ctcgcaaatt
      721 cttccttatt gaaactgatg tcatctgcac cacagccacc ggcaatagca ggaatgatgc
      781 taatggtaga atgctcagaa acagacgttt gttctttctg aaggtcgcgg atatcgtcct
      841 caccgacgaa tatattcaca taaccgcgga tattgccatt gtcctcgagc aaattctttt
      901 taagatcagg gaaatcctga atgagattgt caatactttc tttgacagta ctgccgttta
      961 caagcacttt tgactgttgg tttgtaaatt ttctgagtgg tgtaggaatt acaattgtag
     1021 ccatgatttt ttatttaaat gttatgaaat tattttatgg aaatttccgc ctcgcgggca
     1081 atctgttctt cgtcaaactg aaaatcatcg ttcagtctcc aggattgcag tgatacgaat
     1141 tcgttttcgc gtactgctat tattaaatat gaaaaataag gctgtgcaac aacccggtcg
     1201 tgttcgcttg gcacagaagg atggttcgga tgagaatggt aaactcccaa caaaagcaga
     1261 ttattagctt cggcatattg ttctgctttc atgtagtcga atggcgtaat ttcgaaccgt
     1321 cttcgtttgt ctcctacctt tgcattgttt acaatctgaa tttcggaaat ggttctttct
     1381 tcgccattct cggttccaaa cataaaccca cagcattcat ccggaaagct tcgcaatgcg
     1441 tcagcaagga tttcaatttc tgtttttctt gaaatgtgca acatgatttt acattatttt
     1501 tttgattact tcactgtatt tgtctgcatt atcgggcaaa atcgtgatga tgacaccgct
     1561 ttctatattg tcagccactg tcgctgctcc gactatgttg gctgctgatg aggggctgag
     1621 tacaccaccg aggactttct tcctcatcag gtcatacgcc ttgaaagtgt ctatctccaa
     1681 cccataatct gcaatttgcg catcgtaaat ctccggcacg atggctgtct cgagatgttt
     1741 ccagccttca aggccatgta tggccacatc cggctgcaaa gaaatggctt gtatagccgg
     1801 attgtactct ttgagccttc ttgaagtgcc tacaaaagtg cccgttgtac caagtcctgc
     1861 cacaaaatgt gatatttcgg gcaattcacg gataatttcg atcgctgtgc cgtaataatg
     1921 tgctttccag ttgttgtcat tggtatattg gctggcataa aaatattttt tggggaaatc
     1981 ctttgcgagt tcagctgcct tttcctgtgc ctcgtctgtt ccgccaaacg gagaagtatt
     2041 aataatttcg gcaccgagac tgtgcagaat gtcttttctc tcttgtgatg cattttccgg
     2101 cagacacaat gcgactttga tacccagttt ttgtccgatg gctgcatacg cgatgccggt
     2161 attaccgctt gtggcatcca ggagaatctt gttttcagta agttttcctg atgagattgc
     2221 atttttaatg atattgaatg cggctctcgc ttttacactg ccggacaggt ttttccattc
     2281 agcttttgca aatattttta catctttatt ttggtgcaat ccggttatcg gcagcaaggg
     2341 tgtattccca ataaacctgc taatgtgatc cagtttatgt tttagtccgt gactccctgt
     2401 ttgttgttta tacgatactc tattgctcat ttt
//
LOCUS       DAOQQY010000366         2411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_24378_length_2411_cov_8.146859, whole genome shotgun sequence.
ACCESSION   DAOQQY010000366 DAOQQY010000000
VERSION     DAOQQY010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24378_length_2411_cov_8.146859"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..724
                     /locus_tag="PLE23_12465"
     CDS             80..724
                     /locus_tag="PLE23_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX84125.1"
                     /translation="MIQVASFTYNVFEENTYILFDETKECIIIDPGCNNQTERDDLKK
                     FIDTNMLKPVRLINTHCHLDHIFGNKWVYVTYGLLPEIHEGEWQVLQAAPQVSMMYGV
                     RCDVSPSPVRFISDGDIVRFGNSALKSILAPGHSPASLCFYAENEKFLIGGDVLFYES
                     IGRTDLPGGDYDTLIHSIQNRLMTLPDDIEVHSGHGPSTTIGHERRYNPFINEH"
     gene            complement(745..1083)
                     /locus_tag="PLE23_12470"
     CDS             complement(745..1083)
                     /locus_tag="PLE23_12470"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HMX84126.1"
                     /translation="MKNYIIRLVFIIIAVIIADKILKGFHISSYATAFWVALAMSVLN
                     TFVKPVLQFLAIPVTILTLGLFYLVINVVIVYIAAYLVQGFTISGFLTPLLFSIMVSV
                     AGSFANAIKE"
     gene            complement(1181..2239)
                     /gene="corA"
                     /locus_tag="PLE23_12475"
     CDS             complement(1181..2239)
                     /gene="corA"
                     /locus_tag="PLE23_12475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMX84127.1"
                     /translation="MSKKKRNKVGLSPGSIIYTGKFSNTNACIRITKYSQDHLNEIDL
                     AEFRIDALLPIPNGNLWIDVQGLDQVNLITSIGNEFGLHPILIENIVDVEQRPKFEEY
                     DNYLCLIFDTLTLNEETYSIQNLQNAIILGQDFLISFQEGHDDSFRTVRERMRNTIGK
                     TRSKGSDYLAYALVDAQVDGYYLVMDRLEEEVETLEKNIETSNETDIRQKILHLKKEL
                     IKIRRSIQPLREALGKMSRTDSAYIEASTSIFLKDVFDHLVYLMELTENLKDMLSSIY
                     DLYIAQISMRMNNVMKALTIISSIFIPLSFIASVYGMNFDNMPELRNPHGYFYVLGIM
                     FIILLGMIGYFKKRGWMD"
BASE COUNT          757 a          450 c          471 g          733 t
ORIGIN      
        1 tttgtttgaa agtgaaactt gaccggtaga atacggcttt ttttacggaa ctttgctgca
       61 atgaaccaaa acgaacgata tgatacaagt agcttctttt acgtacaatg tgtttgagga
      121 aaatacatac atactttttg atgaaacaaa agaatgtatc atcatcgacc cgggttgtaa
      181 caatcagact gaacgggacg atcttaaaaa atttatcgat actaacatgc tcaaaccggt
      241 caggttaatc aacacacatt gccatcttga ccatatattc ggcaataaat gggtgtatgt
      301 gacatacggt ttgttgccgg aaatacacga aggcgaatgg caggtacttc aagctgcacc
      361 acaagtaagc atgatgtacg gcgttagatg tgatgtttcg ccttcaccag ttcgttttat
      421 ctcagacgga gatattgtac ggtttggcaa ctcagcgtta aagtcaattc ttgcaccggg
      481 gcattcaccc gcaagccttt gtttttatgc cgaaaacgaa aaatttctca ttggcggaga
      541 tgtattgttt tatgaaagta ttggtagaac ggatcttcca ggaggcgatt atgatacatt
      601 gattcattcg atacagaaca gactcatgac gctgccggat gatatagagg tacattccgg
      661 acacggaccc agtactacca tcggacatga acgcaggtac aaccctttta ttaacgaaca
      721 ttgatgcaat aaaaaacagg aagcctattc tttaatagca ttagcaaaac tgccggctac
      781 cgataccatt atactgaaca gcaaaggtgt aagaaatccg gaaatggtga aaccttggac
      841 caaataagca gcaatgtaaa caatgaccac attaataaca aggtaaaaca aacccagtgt
      901 gagtatggtc acaggaatgg ccagaaactg aagtacaggt tttacaaaag tattaagtac
      961 tgacatagct agtgccaccc aaaaagctgt agcgtaagac gaaatatgga aacccttgag
     1021 tatcttgtca gcaatgatta ctgcaataat gatgaaaaca agacggatga tgtagttttt
     1081 catgtgatgt gccaatttaa gtaattcaaa aataatacaa aaagcaatgc ctgaactaaa
     1141 aatttgcctg attaacatca aataattttg ttcagcacaa tcaatccatc caaccgcgtt
     1201 tcttaaaata tccgatcata ccgagtagta tgataaacat aatgcccaac acgtaaaaat
     1261 agccatgcgg gtttctgagc tccggcatat tgtcaaagtt cattccatag actgaggcaa
     1321 taaaactcaa cggaataaat atagatgaga taatagtcag agccttcatt acgttattca
     1381 tgcgcatact tatctgtgca atgtatagat catagatact gctgagcata tctttgaggt
     1441 tttcggtaag ctccatcaga tagacgaggt gatcaaatac atctttcaaa aatatgctgg
     1501 tagatgcttc aatataagca ctatcggttc tgctcatttt tccaagtgct tcacgcaggg
     1561 gctgtatgct tcttcttatt ttaataagct cttttttcag atgaagtatt ttctggcgaa
     1621 tgtctgtttc gttgcttgtt tcaatatttt tttcgagggt ttcgacttct tcttccagcc
     1681 ggtccatcac gaggtaataa ccatctacct gtgcatctac gagtgcataa gcgagataat
     1741 cggagccttt tgacctggtt ttgccaatag tatttctcat gcgttctctt actgtgcgga
     1801 acgagtcatc atggccttct tgaaagctga ttaaaaaatc ctggcctaaa atgatagcat
     1861 tctggagatt ttgtatgctg taagtttctt cattgagtgt cagtgtatca aaaatgaggc
     1921 ataggtaatt gtcatattct tcaaattttg gtctttgctc cacatccaca atattttcaa
     1981 tgagaattgg gtgtaggccg aattcattgc caatagatgt aattaggttg acctggtcaa
     2041 gaccttgtac atctatccat aaattgccgt taggtattgg taacaaggca tctattctga
     2101 actcagccag gtcaatttca ttgaggtgat cctggctgta tttggttatc cggatgcagg
     2161 cattagtatt gctaaatttt cccgtataaa taatacttcc gggtgatagg cctaccttgt
     2221 ttcttttttt ctttgacact gaagagatag tttgtacaaa attattaaaa atctcattcg
     2281 gaatgtaaat aaaaaagggc aaaagaaaat atcttctgcc ctatctaatt tttatcaaat
     2341 caccaaatgg tgaattattt ttggctatgt tcttaataag tgttgcaata atgcacctga
     2401 tctatatagt t
//
LOCUS       DAOQQY010000367         2407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_24454_length_2407_cov_5.132653, whole genome shotgun sequence.
ACCESSION   DAOQQY010000367 DAOQQY010000000
VERSION     DAOQQY010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24454_length_2407_cov_5.132653"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLE23_12480"
     CDS             complement(<1..522)
                     /locus_tag="PLE23_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84128.1"
                     /translation="MKKILFLALALSGVALYSCKNQAGKTSTASGDRTSFGYEYKILT
                     PGTGTKVINTGDRVAYTMYRYLNDTIQGEPYIAEVELMPGDSVQRQRLPFVDLMYMMK
                     EGDSAQVIQKLDTIQNLPPNLKKTDIIKYVVKIRSVKDAAQVQKDKEAAMAQIAPLQA
                     KLKQTINDFNAKKL"
     gene            complement(519..1535)
                     /locus_tag="PLE23_12485"
     CDS             complement(519..1535)
                     /locus_tag="PLE23_12485"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HMX84129.1"
                     /translation="MENKAEISKLLSSPKDVVIFSHRNPDGDALGSSLALCHYLTGIH
                     HSVKIIFPSEYPIIFTWMPGSDKVLVYDLSPNECIAAIKQANLIFCLDFSSLDRIDKM
                     GEFVNQQTCPKIIIDHHLDPEPFADYIISDTSASSTCEMIFDFISDLHGKEKITEEIA
                     ACIYTGIVTDTGSFKYSTSPAVFRKVADLLEAGVNDVLLQNLIHNRLQEKYLRLLGYC
                     LYEKMEIMPEVKTGLISLSREDYIKFDIQRGDTEGIVNYLLMLENVDIAALITEQPTI
                     VKLSLRSKGDISVQEICRDHFKGGGHKNASGGAVYGTLSQTVKKFKEVIPKYFKPNLS
                     NISK"
     gene            1673..2095
                     /locus_tag="PLE23_12490"
     CDS             1673..2095
                     /locus_tag="PLE23_12490"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMX84130.1"
                     /translation="MKGNRTFTMIKPDAVKAGHIGAILAKINEGGFRIVAMKYTKLSA
                     EKAGEFYAVHSARPFYGELVEFMSSGPIVAAILEKDNAVEDFRTLIGATNPAEAQPGT
                     IRALFAKSIGENAVHGSDSDENAAIECAFHFAGTEIYK"
     gene            complement(2152..>2407)
                     /locus_tag="PLE23_12495"
     CDS             complement(2152..>2407)
                     /locus_tag="PLE23_12495"
                     /inference="COORDINATES: protein motif:HMM:NF013844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMX84131.1"
                     /translation="RSDQEPESKVDLRGMRKEEAVKVLESFIDNALIHNAHMLRILHG
                     KGDGVLKKLVWTKLKEYKDVREVQHPEEEFGGSGVTIVKL"
BASE COUNT          697 a          486 c          450 g          774 t
ORIGIN      
        1 tagctttttt gcattaaaat cgttgatagt ttgcttgagt tttgcctgta atggtgctat
       61 ctgagccata gccgcttctt tatccttctg aacctgtgca gcatctttta cagaacgtat
      121 tttcaccaca tatttaatga tgtcagtctt tttaaggttt ggtggcaaat tctggattgt
      181 atcaagtttc tgaattacct gtgcactgtc accttctttc atcatgtaca tcaagtcaac
      241 aaatggaagt ctttgtcttt gcacagaatc acccggcatc aattctactt ctgcaatgta
      301 tggttcacct tgtattgtgt cattgaggta acggtacata gtatatgcta cacgatcacc
      361 ggtattgata acttttgtgc cagtacccgg tgtaaggatt ttatactcat aaccaaaact
      421 tgttctgtca ccgctagctg ttgatgtttt gccagcctga ttcttacaag agtaaagagc
      481 tactccggac aatgccagag caagaaagag gatttttttc attttgaaat gtttgataaa
      541 ttaggtttaa aatatttagg aataacttct ttaaattttt taacagtctg gctgagtgta
      601 ccataaaccg ctccgcctga tgcatttttg tgcccgccac ctttgaagtg gtcacgacat
      661 atttcctgta cggatatatc accttttgat cgaagtgaca gctttacgat agttggctgt
      721 tcggtgatta aggcggcaat gtcaacgttt tcgagcatca aaagataatt aacgatacct
      781 tctgtatcac cacgctgtat gtcgaatttt atatagtctt ctcttgatag ggatatgagc
      841 ccggttttaa cttccggcat gatttccatt ttctcataaa ggcaataacc aagaagacgc
      901 aagtatttct cctgcaatct attatgtata aggttctgaa gcaatacatc attcactcct
      961 gcttcaagca aatctgcaac tttacgaaaa acagctggag aggttgaata cttaaatgat
     1021 cctgtatcgg tcactattcc ggtataaatg catgctgcaa tctcctcagt aattttttct
     1081 ttaccatgca gatccgaaat aaaatcaaaa atcatctcac aagtcgaact tgcagaggta
     1141 tcagaaatga tatagtcagc aaatggttcc ggatcgaggt ggtggtcaat gattatcttt
     1201 gggcaggtct gctgatttac aaattcgccc attttatcga tcctgtccag cgatgagaaa
     1261 tctagacaaa atataagatt tgcctgcttg atggctgcaa tacattcatt tggcgacaga
     1321 tcgtacacga gtactttatc ggaacccggc atccaagtaa aaatgatagg atattcggat
     1381 gggaaaatta ttttaacact atgatgtatg cctgtcaaat aatgacataa cgccagcgac
     1441 gaaccaagtg catcaccatc aggattacgg tgcgaaaaaa taactacgtc cttagggctg
     1501 cttagtaatt ttgatatttc tgctttgttt tccaacttct ttccaaaata aagtgcgaaa
     1561 ttcgtaaaat ttattcactt aaacaaaatt ttattttgct aaatatagtt tatcattgaa
     1621 atgaccttta actttgcgcc tcattttatc aaaatcaagt aattaacaat atatgaaagg
     1681 taacagaact tttaccatga ttaagcctga tgctgttaag gccgggcaca ttggtgctat
     1741 tttagcaaaa ataaatgaag gtggcttcag aatagttgcc atgaaataca ccaagctatc
     1801 ggctgaaaaa gcaggcgaat tttatgctgt tcacagtgca agaccattct acggtgagct
     1861 cgttgaattt atgtcatcag gtcctatcgt agctgccatc cttgaaaaag acaatgcagt
     1921 ggaagatttc agaacattga ttggcgcgac gaacccggcc gaagctcaac ctggtaccat
     1981 ccgtgctctg tttgctaaaa gtattggtga aaatgctgta cacggttcgg attctgacga
     2041 aaatgctgcg atagagtgtg catttcattt cgcaggaaca gaaatctata agtaagagct
     2101 tataaatcaa agaattttct gaaagccacc ttatcatttg ggtggctttt cttataattt
     2161 cacaatcgta acaccactac cgccaaactc ctcctcagga tgttgaacct cccgtacatc
     2221 tttgtattct tttaattttg tccagactaa tttcttcagc acaccatcac ccttgccatg
     2281 caatattctg agcatgtgag cattgtgaat aagagcatta tcgataaatg attcaagtac
     2341 ttttactgct tcttcctttc tcattccacg cagatcgacc tttgattcgg gctcctgatc
     2401 agatcgt
//
LOCUS       DAOQQY010000368         2356 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_25347_length_2356_cov_5.081704, whole genome shotgun sequence.
ACCESSION   DAOQQY010000368 DAOQQY010000000
VERSION     DAOQQY010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25347_length_2356_cov_5.081704"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="PLE23_12500"
     CDS             complement(<1..484)
                     /locus_tag="PLE23_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84132.1"
                     /translation="MTQIVDPIAQSLGLTVERVGDNPADTATVREIRFEQPAQQQQPQ
                     APAEPVQTPAEPAAPVAAAAEPAAPAPVDLKSIFGEEYGEVDKVKTLLGEVPTLREKI
                     ADYEKTVKAVEEDPYIKSRLSWRQEGRDDSLHDLVYYSNPEKMSPEQKVALKLQIENG
                     L"
     gene            complement(1067..1963)
                     /locus_tag="PLE23_12505"
     CDS             complement(1067..1963)
                     /locus_tag="PLE23_12505"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMX84133.1"
                     /translation="MIAGKNILVTGGCGFVAGRIAEVFGLYNNVTVMDNMRYWQDMPQ
                     VIPPRIVEMWEHDLTDPLSLDHFDIVIHGATVNIIHGMSDMRACVDTNFTQTLAFFDR
                     IPQKTPVLYLSTSSVYGNGVMIPTDEKCRVAPSNVYAMTKLLAEQYLLEHHPNCVIAR
                     LSNVYGPRQRPENAYSGVMVKLLTSALWGRPFKVYGDGSQTRDFTYVDDVVAACVALL
                     ESGKRGEVYNVGSGVETSVAQLMKYLPENVEVEITEPRSIDNISRRCLDVRKIAMDTG
                     WKPAVSLDHGVAETMLWLKGVM"
     gene            complement(1960..2139)
                     /locus_tag="PLE23_12510"
     CDS             complement(1960..2139)
                     /locus_tag="PLE23_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84134.1"
                     /translation="MTLSPMFSPKMGLIYFLLLFIGAVVLNNSFATPWIILGGTMLVI
                     GMVGVFFSIVNNYRK"
     gene            complement(2136..>2356)
                     /locus_tag="PLE23_12515"
     CDS             complement(2136..>2356)
                     /locus_tag="PLE23_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84135.1"
                     /translation="EVAPLYGWIETACDAHMKKLGMKWVMLPDYRVQHIASSPLAQEW
                     KNWMVGPGMDLGQITFEQWLREYKGAKG"
BASE COUNT          542 a          631 c          545 g          638 t
ORIGIN      
        1 tcaacccgtt ctcaatttgc agtttgagtg ccactttttg ctccggcgac atcttttccg
       61 gattgctgta ataaaccaag tcatgcagac tgtcgtcgcg gccttcttgt cgccaggaca
      121 aacgactctt gatgtacggg tcttcttcga ccgccttcac cgtcttctca taatcggcaa
      181 ttttctcccg aagcgtcggt acctctccca acagggtctt gaccttatcc acctctccgt
      241 actcttctcc aaagatcgac ttcagatcaa caggggcagg ggcggcaggc tcagcagcgg
      301 cggcaactgg tgccgccggt tctgccggag tttgtactgg ctcagcggga gcctggggtt
      361 gttgttgttg tgctggttgt tcaaagcgga tttcccttac ggttgcggta tccgccggat
      421 tatcgccaac tcgctcgacg gtcaggccga ggctctgggc aatagggtca acaatttgtg
      481 tcatagtgtt gtggtttatg cgttaagact cgccacaaga gatcccactt gctgatcggc
      541 acgaatcgct tccacaacag tctgtgttgc gttctcaatg cccacaaggg agtcaaatgt
      601 agtagtcggg tatgcgtagg cgtacacaag ctcagagaca ttaatgctca gtgcctgcgt
      661 ggtgctgtct gcgttgatct ttgtgatcgc aatgtttgtg atagagttcg acgtgttcgc
      721 cgtgttcaca ctcgcacgaa tatcagaggc ggcttgcgtt accacaacct gatatggatt
      781 actgccatca ccaaccatat acttgattgt gcaacagtta attactgacc tgttataggt
      841 catagtgcct tgtgtgaggt caaggatacg agccgacatg tacatgcagg acttcgctga
      901 tgggtaggta accccgttct tgactatttc ggaaaggact aatgatcttg ccattttatt
      961 tgatgttttt gcaaatgtat ggtcatatca tgcccgtttt tcgcttctac gaaaattttc
     1021 ttgattggta cccacaaaga tatgtagtca gcgcccgtat cccaaattac attacgccct
     1081 tgagccaaag catcgtttcg gccacaccgt gatcaagact caccgccggt ttccaccccg
     1141 tatccatagc tattttacgt acatcaaggc accgccttga gatattgtca atgctacgag
     1201 gctctgtgat ttccacctct acgttttccg gtaaatactt catgagctga gctacgctgg
     1261 tctctactcc ggagcctacg ttataaacct cgcctcgctt accggattcc aaaagcgcca
     1321 cgcaggctgc taccacatca tcaacatagg taaaatcccg ggtctggctt ccatcaccat
     1381 acaccttgaa gggtcgaccc cataacgccg aagtcaatag cttgaccata acccctgagt
     1441 atgcgttctc tgggcgttgg cgcggaccgt acacgttact taaccttgca atcacacagt
     1501 tggggtggtg ttcaagtaag tattgttcgg ccagtagctt agtcatggca tacacgttgc
     1561 ttggtgccac cctgcacttt tcgtcagtgg gtatcatcac tccgttgcca tataccgagc
     1621 ttgtgctgag gtaaagcact ggtgttttct gtggaatacg gtcaaagaac gccagggtct
     1681 gcgtgaaatt agtgtccaca cacgcccgca tgtcgctcat gccgtgaatg atatttacag
     1741 tggcgccatg aatgacaata tcaaaatgat caagactgag cggatcagtg aggtcatgct
     1801 cccacatctc cacaattcga ggcggaatca cctgcggcat gtcctgccag taccgcatgt
     1861 tatccatgac cgtgacgttg ttgtacagtc caaatacctc tgcgatacgg ccagctacaa
     1921 agccacagcc tccggtaacc agtatgtttt taccggctat catttacgat agttgttgac
     1981 aatactgaag aataccccga ccatgcctat tacaagcata gtccccccaa ggataatcca
     2041 tggtgttgcg aaactgttgt tcagcactac tgccccgata aacagcagta agaaataaat
     2101 caaacccatc ttagggctga acattggtga tagtgtcatc cttttgctcc tttatattcc
     2161 cgtaaccatt gttcaaatgt aatctgcccg aggtccatgc cagggccaac catccagttc
     2221 ttccactcct gcgccaaagg tgaggaggcg atgtgctgaa cccggtaatc cggtagcatc
     2281 acccacttca tcccaagttt cttcatgtgg gcatcacagg cggtttcaat ccatccgtac
     2341 agcggcgcta cctctg
//
LOCUS       DAOQQY010000369         2349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_25448_length_2349_cov_6.442459, whole genome shotgun sequence.
ACCESSION   DAOQQY010000369 DAOQQY010000000
VERSION     DAOQQY010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25448_length_2349_cov_6.442459"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1504)
                     /gene="cysS"
                     /locus_tag="PLE23_12520"
     CDS             complement(17..1504)
                     /gene="cysS"
                     /locus_tag="PLE23_12520"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMX84136.1"
                     /translation="MSSVLQIYNSLARQKEIFDPVNPPYVGLYVCGPTVYNDVHLGNV
                     RTFISFDMVYRYLKYLGYKVRYVRNITDAGHLTNERGEGVNRIESQSQLEKLEPMEIV
                     QKYTVGFHDVCRLFNLHRPTIEPTATGHIIEQIEMAKQLINNGYAYESNGSVYFDVNA
                     YNSHNHNYGKLSGRNTEELIAGYRDLDGQDEKRNAIDFALWKKASPEHIMRWPSPWGE
                     GFPGWHLECSVMSTKYLGTTFDIHGGGMDLKFPHHECEIAQNIGATGTEPVKYWMHTN
                     MLNFNGQKMSKSLGNSILPLELITGNHPLLDKAYSPMTVRLLFLQSHYSSELDITLKG
                     LQDAEKGLRRLLNAAQYLDGLCLSGETNTTTDDELNQLADKCKEVMDDDFNTAKLIAT
                     LYEMASRINIFHNDNKKCPVSEPTFTRFRDIFKAFLFEVLGIVNENEATSTDTLDKVM
                     NLVIDIRKTARENKDWPTSDKIRDALKDAKIILKDNKDGTSYEVE"
     gene            complement(1515..>2349)
                     /locus_tag="PLE23_12525"
     CDS             complement(1515..>2349)
                     /locus_tag="PLE23_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMX84137.1"
                     /translation="VIGFNSVKGVINFGSKNQRTTGKSITVGTYNIEGLMRLNPKSKE
                     YRKLSKDDLKEFFDKNKIPDVICFQEITTNNRKTLQSILDLPYFYQHTSLNTCIFSKY
                     PITDSGSIVFSKSYNSAVWVDIEINGKKIRVFDFHLQSNTVKNLTEKTLSSEELQARK
                     KYSSIRKIFSRIKHATDLRVNQAKQVKALIRKSPYPVLVCGDMNDTAQSYVYATISDG
                     LKDSFKESAFGFGSTYNGKLPFLRIDYIFTSEEMSVLSHKVERVIFSDHNPVFSTISL
                     N"
BASE COUNT          597 a          463 c          509 g          780 t
ORIGIN      
        1 tacctgagca ggtgaattat tctacttcgt aagaggtgcc atctttgttg tccttcagta
       61 tgatttttgc atctttcagt gcgtccctaa ttttatctga ggttggccag tctttgtttt
      121 cgcgggcagt tttgcgaatg tcgattacca gattcatcac cttgtcgagc gtatctgtac
      181 ttgtagcttc attttcatta actataccaa gcacttcaaa caagaatgct ttgaaaatat
      241 ctctgaaccg ggtaaaggtt ggttcgctaa caggacattt tttgttgtcg ttgtgaaaaa
      301 tgttgatacg tgatgccatt tcgtatagcg tagcaatgag tttggccgta ttgaagtcgt
      361 cgtccatcac ttccttacat ttgtcggcaa gctggttcag ttcatcatcc gtggttgtat
      421 ttgtttcacc agataaacag agcccgtcaa gatactgagc agcattgagc aatcggcgaa
      481 ggcctttttc ggcatcctgt agccctttta gagtaatgtc cagctcagac gaataatgcg
      541 attgcaagaa caataacctc accgtcattg gcgaatatgc tttatcaagc aatggatgat
      601 tgcctgtgat gagttcaaga ggtagtattg agttaccaag cgatttgctc attttctggc
      661 cgttgaagtt cagcatattg gtgtgcatcc agtattttac aggttcggtg cctgtagcgc
      721 ctatattctg tgctatttcg cattcgtggt gaggaaattt gaggtccata ccgccaccat
      781 gtatatcaaa ggtagtgccc aaatatttcg tactcatgac agaacattcg agatgccatc
      841 ccgggaatcc ttcgccccat ggagatggcc atcgcattat atgttcgggt gatgcttttt
      901 tccacaaagc gaagtcaata gcgtttcgtt tttcatcctg tccgtccagg tcgcggtatc
      961 cggctatcag ttcttcggta tttctgcctg aaagcttgcc gtagttatga ttatggctgt
     1021 tataggcatt aacatcaaaa taaaccgagc cattcgactc ataagcatac ccattgttaa
     1081 tcaattgttt agccatttct atttgttcga taatgtggcc ggtggcagta ggctcaatag
     1141 tagggcgatg taggttgaat aaacggcaga catcatggaa gccgacagta tatttttgca
     1201 ctatctccat tggttcgagc ttttcgagct gcgactgtga ttcgatacgg ttgacgcctt
     1261 cgcctcgttc gttggtgaga tgaccagcat ctgttatgtt gcgtacgtac cgtaccttgt
     1321 agcccaaata ttttaggtaa cggtacacca tgtcaaaact gatgaatgta cggacattgc
     1381 ccagatggac atcattatat acagttgggc cgcacacata caggccaaca taaggtggat
     1441 tgacagggtc gaatatttcc ttttgccttg ccaggctgtt atatatctga aggacagaac
     1501 tcatatttat tcaattaatt taatgaaatt gttgaaaata ccggattgtg gtcagagaaa
     1561 atgacacgtt ctactttatg ggacaaaacc gacatttctt cacttgtgaa gatataatcg
     1621 attctgagaa acggaagttt accattataa gtagagccga atccgaaggc actttcctta
     1681 aaactatctt taaggccgtc acttattgta gcatatacat aagactgtgc tgtatcgttc
     1741 atgtcaccac ataccaaaac cgggtaaggt gacttacgta ttaacgcttt tacttgtttc
     1801 gcttggttga cccttagatc tgtggcgtgt tttattcttg aaaaaatttt ccttatactg
     1861 ctgtattttt tccttgcctg cagttcttca gaagagaggg ttttctctgt gaggtttttt
     1921 acagtattgc tttgcaaatg gaagtcgaat acgcggattt tttttccatt aatttctata
     1981 tccacccaga ctgcagagtt gtatgatttg gagaatacga tactcccgct gtctgtaata
     2041 gggtattttg aaaagataca agtgttgagt gaagtatgct ggtagaaata cggcaaatca
     2101 agtatgctct gaagtgtttt tcggttattg gtggttattt cctgaaagca aatgacgtca
     2161 ggaattttat ttttatcgaa aaattctttc aggtcatcct ttgaaagctt gcggtattct
     2221 tttgattttg gattgaggcg catgaggcct tcgatgttat aggtacctac tgtaatgctc
     2281 ttacctgtag tacgttgatt tttgctgcca aagtttatta caccttttac actattaaag
     2341 ccaatgacc
//
LOCUS       DAOQQY010000370         2344 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_25540_length_2344_cov_4.551769, whole genome shotgun sequence.
ACCESSION   DAOQQY010000370 DAOQQY010000000
VERSION     DAOQQY010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25540_length_2344_cov_4.551769"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="PLE23_12530"
     CDS             complement(<1..1456)
                     /locus_tag="PLE23_12530"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84138.1"
                     /translation="MLQRNNVILMASAICLCLSWVAVKQFVKPDAYVQVKKHKGRPYE
                     DFWMKRSFPSFEPESRAYFQALDDVREAMQLRGSIPGFDKEWTTEGPGNIGARVNSIA
                     VNPVDENTILIGYSHGGIWKTTDGGQNWHPVFDEQSALAIGDLAFDPKNPNVVYAGTG
                     DVNIPGGVYTGNGVYKSTDGGESWEPSGLEETAIISKIWVDPTNSAVVFASGMGSPFV
                     KNTERGLYKSIDGGQNWQKILFVNTSTGIIDFEVNPQNPDYIYAAAYTRIRTNYESIV
                     SSKDCSVWRTIDGGKNWTQPGNGLPLGGSHSRIGLAMCPTDPSNVYAMYVSSDLDLEA
                     IYKTTDAGSTWAEVPTYDTGVPEGQMGGFGWYFGKLGIDPNDCNTLHLLGVDMQSTFD
                     GGASWVESVPPWWTYEVHADKHDIAYTASGNVLLGTDGGLYKTSDFNTWEKIENNPTS
                     QFYRVAWNPHQPDFYYGGMQDNGTSGGNAAGINSW"
     gene            1608..2270
                     /locus_tag="PLE23_12535"
     CDS             1608..2270
                     /locus_tag="PLE23_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84139.1"
                     /translation="MRVCTASILLCLLSLSGFGQISLGNPSFESKPGNAAIPDKWVTC
                     VPGSTPDILPGVWGVNKAPQDGMSYLGLITRENGSKESIGQRLSKPLEANTCYTFSVY
                     LARSETYVGYNLPLRLKIWGSKEYCGKDQLLVSTKAIKHTNWEQYEFQIYTKAKVHFL
                     LLEACLAPGVTIPYRGNILIDELSPIKPCFKAENNHIQQADFFGWATSIFSYLIDWVA
                     GN"
BASE COUNT          572 a          668 c          477 g          627 t
ORIGIN      
        1 cccagctatt aataccggca gcattacctc ccgaagttcc attgtcctgc ataccgccat
       61 aataaaaatc gggctggtgt gggttccagg ccacgcggta aaactgcgat gtcggattgt
      121 tctcaatctt ctcccaggta ttgaagtcag acgttttgta cagacctcca tctgtaccga
      181 gcaacacatt gccggaggct gtatatgcta tatcgtgctt gtcggcatgt acttcatagg
      241 tccaccaggg tggcacagac tctacccagc ttgcaccacc gtcaaaagta ctctgcatat
      301 ctacaccgag gaggtgaagc gtgttgcagt cgttcgggtc aatacccaat ttgccaaaat
      361 accagccaaa tccacccatc tgaccttcag gtacaccggt gtcgtaagtc ggcacttcag
      421 cccatgtact gcccgcatct gttgttttgt atatagcttc cagatcgagg tcactgctca
      481 catacatggc atatacattc gaagggtcag taggacacat cgcaagccca atcctcgaat
      541 ggctgccacc aagcggcaga ccattgccgg gttgtgtcca gttcttccct ccgtcaatgg
      601 ttcgccatac cgaacaatct ttactcgata cgatgctctc gtagttggtg cgtatccgtg
      661 tgtaggccgc agcatagata taatccgggt tctgagggtt gacctcaaaa tcaatgattc
      721 cggtagaagt attaacaaag agtatcttct gccagttctg accaccgtcg atacttttgt
      781 atagcccgcg ttcagtattc ttcacaaacg ggcttcccat tcccgaagca aagaccactg
      841 cactatttgt agggtccacc catatttttg atatgatggc cgtctcctcc agacccgatg
      901 gctcccagct ttctcctccg tcagtgcttt tgtacacacc attgcctgta tatacaccac
      961 ccggtatatt tacatcacct gtacccgcat atactacatt cggattctta ggatcaaaag
     1021 ccaaatcacc gattgcaaga gccgattgtt cgtcaaatac cggatgccag ttttgtccgc
     1081 catccgttgt cttccagata cctccgtgcg aataccctat cagaatcgtg ttttcatcca
     1141 ccggattcac agctatggag ttgactctgg cgcctatatt ccccgggcct tctgtcgtcc
     1201 attccttgtc aaaacccgga atgctccctc tgagctgcat agcctcccgc acatcatcca
     1261 atgcctgaaa ataagcccgt gactccggct caaacgacgg aaagcttcgc ttcatccaaa
     1321 aatcctcata aggcctgcct ttgtgtttct ttacctgcac ataggcatct ggctttacaa
     1381 attgtttgac tgccacccac gatagacaca gacagatggc gcttgccatg agtataacat
     1441 tattcctttg gagcatgaac tacttatttt tctgcaaggt aaactttttt ctttaaaaat
     1501 ttcactacct ccccgcttta agttacaagt tcactcagta ttcacaacat atacatatta
     1561 ttatttacat acatccgttt tccatcagta tgtcatacct ttgcatcatg agagtatgta
     1621 ccgcatcgat tttgctgtgc ctgttaagct tgtctggctt cggacagatc tcgcttggca
     1681 atccttcatt cgaatcgaag ccgggcaatg ctgccatacc cgacaaatgg gtcacctgcg
     1741 taccgggctc cactcccgac atattgcccg gtgtgtgggg tgtcaacaag gcaccacaag
     1801 acggcatgag ctatctcggc ctcatcactc gcgaaaacgg cagcaaagaa agcatcggcc
     1861 aacgtttgtc aaagccactc gaagccaata cctgctacac attttctgta tatctggcca
     1921 ggtcagagac ctacgtcggt tacaacctcc ctttgcgcct taagatttgg ggcagcaaag
     1981 aatactgtgg caaggaccag ctgctggtaa gcaccaaagc catcaaacat accaactggg
     2041 aacagtacga gttccagatc tacaccaagg ctaaggtaca ctttctcctg ttagaggctt
     2101 gccttgcccc cggtgtcacc ataccatacc gcggcaacat cctcatcgac gaactttcac
     2161 ctatcaaacc ctgcttcaaa gccgaaaaca atcatataca gcaggccgac ttcttcgggt
     2221 gggcaacatc catcttttcc tacctgatcg attgggttgc aggcaattaa tacatgtcat
     2281 gttttatttg ggcgtgcccc tgtcgggtca ggctatccgc cttacttcgt gccggctcct
     2341 atcc
//
LOCUS       DAOQQY010000371         2343 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_25557_length_2343_cov_5.900350, whole genome shotgun sequence.
ACCESSION   DAOQQY010000371 DAOQQY010000000
VERSION     DAOQQY010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25557_length_2343_cov_5.900350"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2343)
                     /locus_tag="PLE23_12540"
     CDS             complement(<1..>2343)
                     /locus_tag="PLE23_12540"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HMX84140.1"
                     /translation="ITTTPESCIGTKDGEIKLTVTNATGSLSYSLNGANPVSNGTFTN
                     LASGAYSIIIQDGNGCKDTIQADIKTAPPITISKTVKDVSCFGLSDGSISVEAFGGSG
                     VYTYNWIGNAATGPNATSLSAGIYTLFISDNKNCSVTDTFYVKQPTQLTSVPFIQKPS
                     CFGFTDAYIDVQSQGGVKPYQYNWSDGSGQGIKTGLGAGTYKVTITDVNGCSVVKNIL
                     VTQPAALKDTVDVIQPSCFGDANGSIEIKLGGGTSPYSFNWNEFPLNNSSKVNGLSSG
                     KYSVTASDANGCKVAKTVTLVEPAKVEAIADAEDESCPGLNDAMAEIVISSGKGPFTY
                     TWPNGVTANGNKAMNLLPGNYSVEVANAKGCKTTVNFTVNAAAPFAGFVDAPLIDCKS
                     TSGIATVVITDGVAPYTYLWNDPLAQTTSQATSLATGNYKVSITDSRNCQLVLDANVT
                     SNDPVLVDQISVNNTSCNNTTDGSAQLSFKGGTLPYTITWNNGIPANDFNPQNLASGT
                     YEYIVTDNVGCTLKGDIIINAPEPAILTAQSTDAACKFGATGKIDLTVTGGTPPYAAS
                     WSNGASGLNIDKLTAGAYTVTVTDNKNCNYTQLFSISEPAEEFTIEATQKYVSCYGQK
                     QGVAEVKMLSGSGTIATVLWNTAATVQTIKYLAPGNYSVTVTDSYSCKQSKSINVNEW
                     QAVTFDVTTTKPTCNNTTDGKIIVTNINGGAGAGDLANYQVSWNNQVPGQNFTQKNLA
                     CGQNVTIKVIDQQGCATTKDVGVPVTQAIDYIVTEKEPTCY"
BASE COUNT          614 a          502 c          486 g          741 t
ORIGIN      
        1 atagcatgta ggttcttttt cggtgactat ataatctata gcctgagtga ccggtacgcc
       61 aacatctttg gtagttgcac aaccttgctg gtcaataacc ttgatggtta cattctgtcc
      121 gcaagccaga tttttttggg taaaattctg tccaggcacc tgattattcc atgacacctg
      181 atagttggcc aaatcaccgg caccggcgcc accatttata ttggtgacaa taattttacc
      241 gtcagtagtg ttattgcatg tgggctttgt ggttgtcaca tcaaacgtga ctgcttgcca
      301 ttcatttaca ttaatgcttt tcgattgttt acaactataa gaatctgtaa ctgtcacaga
      361 ataattgccc ggtgcaagat atttaattgt ctggacagta gcggcagtat tccaaagtac
      421 ggtggctatc gtacctgatc ctgaaagcat ttttacttct gctacacctt gtttttgccc
      481 ataacatgat acgtattttt gggtggcctc aatggtaaac tcttctgccg gctcagagat
      541 actgaacagc tgagtataat tacaattttt attgtctgta acggttacgg tataagctcc
      601 agcagtaagc ttatcaatgt tgagaccgga agctccattg ctccaacttg ctgcataagg
      661 cggcgtacca cctgtgactg tcagatcaat tttaccggta gctccaaatt tacaagctgc
      721 atccgtactc tgtgctgtga gaatagctgg ttcgggcgca tttatgataa tatcgccttt
      781 caatgtacaa cctacattat cagtaacgat gtactcgtag gtacccgaag cgagattttg
      841 agggttaaaa tcattagcag gtatgccatt attccaggtg atggtatatg gtagtgtacc
      901 acctttaaaa cttaattgtg cactaccatc agttgtatta ttgcatgaag tgttgtttac
      961 tgaaatctgg tcaactaata ccgggtcatt agatgtaaca ttggcatcaa gtaccagttg
     1021 acaattgcgc gaatctgtta tacttacttt ataattaccg gttgcgagtg aagtcgcctg
     1081 gctggtagtc tgtgccagcg gatcattcca caggtatgtg tatggcgcta ccccatctgt
     1141 gattactaca gttgcaatac cacttgtgct tttgcaatca atgagcggtg catcaacgaa
     1201 gccggcaaat ggtgctgctg cattaactgt aaaattaact gtcgtcttac atcctttcgc
     1261 atttgctact tcaactgaat aattgcccgg caacaaattc atagctttat tgccattggc
     1321 tgttacacca tttggccatg tgtatgtaaa cggaccttta ccgcttgata ttacaatttc
     1381 agccattgca tcatttaagc ccggacatga ttcatcttca gcatcagcta ttgcttcaac
     1441 tttggcaggt tctaccagtg ttacagtctt tgcaacttta caaccattgg catctgaggc
     1501 agtgactgaa tatttccctg aagacaatcc gttaacctta gaactattgt tgagtggaaa
     1561 ttcattccaa ttgaatgaat atggagaagt accaccgccc agttttattt caattgagcc
     1621 attggcatca ccaaaacaac ttggctgaat cacgtcaact gtatctttta atgctgccgg
     1681 ttgtgttacc agaatgttct ttaccactga gcaaccattt acatctgtaa tggttacttt
     1741 gtaggtaccg gctccgagcc ctgtcttgat accctgcccc gatccgtctg accaattata
     1801 ttgatatggt tttacacctc cttgactctg tacatcgata taagcgtcgg taaagccaaa
     1861 gcatgaaggc ttctgaataa aaggcactga agtgagctga gtaggttgct tgacgtagaa
     1921 ggtatctgtg acagagcagt ttttgttatc agaaatgaaa agtgtataaa tacctgccga
     1981 caatgatgtt gcatttggac cggtagctgc attacctatc caattgtagg tataaacacc
     2041 acttccgccg aaagcttcaa cagaaatact gccatcgctt aacccgaagc aactcacatc
     2101 tttgacagtt ttactaatcg ttatgggtgg tgctgtcttt atatcggcct gtatggtatc
     2161 tttacaacca ttgccatcct gaatgatgat ggaataagca ccagaggcaa gattggtgaa
     2221 tgtaccgttg ctgacagggt ttgcaccatt gaggctatag ctgagcgatc cggttgcatt
     2281 tgtaaccgtt aattttattt caccgtcttt tgttccgata caagattctg gagtggttgt
     2341 tat
//
LOCUS       DAOQQY010000372         2324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_25870_length_2324_cov_5.634641, whole genome shotgun sequence.
ACCESSION   DAOQQY010000372 DAOQQY010000000
VERSION     DAOQQY010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25870_length_2324_cov_5.634641"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1381
                     /locus_tag="PLE23_12545"
     CDS             23..1381
                     /locus_tag="PLE23_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMX84141.1"
                     /translation="MKNITTNTIRFLIYVLFYSHAYAQDVKHLSLQEAINTGVANSKQ
                     LKAANAGIEAASWHIRELNNIKLPEINASGAYMRMLQPSFDLKIPLPGSNPQDTSSNM
                     GQGAKALKVNQMMYASVNASLPLFSGFRIRNGIEAAQLLKRATELDADKDKDKVILNI
                     IASYYNLYKAQSAIKLLNENLKQAQSRVKDFTNLEKNGLLARNDLMKAELQQSNVELT
                     IAEAENNLKVSQYSINLLLGLPENTVLELENAGPPSLTQLQPLEEWQKTAIASRADLL
                     ALQTRQQAASKNTESIKGEYYPSVALTGGYIAGYIPNILTLANAANIGIGLSYNISSL
                     YKTGTKVKQAKAQEQQLYWNNLQAQDGIRVQVVKAYEDYNQAMQKITLYEKAVDQANE
                     NYRITKNKFDNSLETTTNLLDADVLQLQARLNYEFAKADAVIAYNKLAETAGTIEKQS
                     LK"
     gene            1438..>2324
                     /locus_tag="PLE23_12550"
     CDS             1438..>2324
                     /locus_tag="PLE23_12550"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HMX84142.1"
                     /translation="MGNKEQKHAAEAEERKKSSRKFAIIFAILLLAGGSYGTVKYLHG
                     LKHEETDDAQVESLISPVIPRVPGYISEVRVRDNQMVHKGDTLLILDDRDYKLRLLQA
                     EAILNGAHTNVSVADAGVQVVSDNIVSSASNINTIEAQIDAAKVNLWRATQDFGRYEN
                     LIKDHSITQQQYEQALAAKQSAERQLDVLQSQKQTASRQTKAVSSQKKVQAEQVAAAN
                     SAIKLRQAEVEMAKLNLSYTVITAAVDGQISKVNLQPGQFIQAGQSLFNIVPDKEKWV
                     VANFKETQLAKMNIGQKVS"
BASE COUNT          819 a          505 c          478 g          522 t
ORIGIN      
        1 tattatctca caaacaaata aaatgaaaaa tataacgaca aacactatcc ggtttctcat
       61 ttatgtgctt ttttattcgc atgcttatgc acaggatgtc aagcatctta gtctgcagga
      121 ggctataaat acaggcgtgg ccaacagcaa acagctaaag gcagccaatg ccggtatcga
      181 agcggcctcg tggcatatac gggagctgaa taatattaaa ttgcccgaaa taaatgcatc
      241 aggtgcctat atgcggatgt tacaaccaag tttcgatcta aagattcctc taccgggcag
      301 taaccctcaa gatacgagtt cgaatatggg gcaaggtgcc aaagcattga aggtaaacca
      361 aatgatgtat gcttctgtta acgcaagcct tcctttattt tcaggattca ggatacgcaa
      421 cggtatcgaa gcggcacaat tgctgaagcg tgccactgag cttgatgcag ataaagataa
      481 ggacaaagtc atcctgaaca tcattgcctc atattataac ttgtacaaag ctcaatcggc
      541 aataaaactc ctaaacgaaa acctcaaaca agcacaaagc cgcgttaaag attttacaaa
      601 cctggaaaaa aatggcctac tggcacgcaa cgatcttatg aaagcagagc tacaacaatc
      661 gaatgtagag cttacaattg cggaagccga aaacaatctc aaggtaagcc aatacagtat
      721 caatcttctt cttggcctcc cggagaatac agttttggag ctggaaaatg ccgggccacc
      781 atccttaacc cagcttcaac ctttagaaga atggcagaaa acggccattg catccagagc
      841 agatctgctg gcattacaaa ccagacaaca agcagcctct aagaacacag aatcaatcaa
      901 aggtgaatat tatccatctg tagcactcac cggtggatat atagccggtt atatcccgaa
      961 tatactcaca ttggctaatg ctgccaatat cgggataggc ctcagctaca atatttcatc
     1021 attgtacaaa acaggaacaa aggtaaagca ggctaaagct caggagcaac aattatactg
     1081 gaataacttg caggcacagg atggcatccg tgtacaagtt gtaaaagctt atgaagatta
     1141 taatcaggca atgcaaaaaa tcacacttta cgaaaaagca gtagatcaag ccaacgaaaa
     1201 ttaccggata acgaaaaaca aatttgacaa ttcgctcgaa accactacta atctgctcga
     1261 tgccgatgtg ttgcagctac aagccagact caactacgaa tttgcaaaag cagatgctgt
     1321 catagcctac aacaagctgg cagaaacagc aggaacgatt gaaaaacaat cattaaaata
     1381 aaagaccagg acagtattta aattgaaatt acactaatat tattaaaact aacaataatg
     1441 ggaaataaag aacaaaaaca cgcagcagag gctgaagaac ggaaaaaatc gagccggaaa
     1501 tttgctatca tatttgctat tctattgctt gcaggtggca gttatggtac tgtcaaatat
     1561 ctacatggtc tgaagcatga agaaaccgac gatgcccagg tcgagtcatt gataagtccg
     1621 gtaatacctc gggtaccggg ttacattagt gaagtacgtg tccgtgataa tcaaatggta
     1681 cacaaaggcg atacactatt gatactcgat gaccgcgatt acaaactgag attactgcag
     1741 gctgaggcta tactaaatgg agcacacacc aatgtttcgg tagctgatgc aggtgtccag
     1801 gtagtatctg acaatatagt gagctcggca tctaacatca ataccattga agctcagata
     1861 gatgctgcca aagtaaatct gtggcgtgcc acccaggact tcgggcgtta cgaaaacctt
     1921 attaaggatc acagtattac acaacagcaa tacgaacagg cacttgcagc caaacaaagt
     1981 gccgaacgcc agctcgatgt actacaatcg caaaaacaga ccgcttccag acaaaccaaa
     2041 gctgtcagct ctcagaaaaa agttcaggcc gaacaggtag ccgctgccaa cagcgccatt
     2101 aaactacgac aagccgaagt agagatggct aaattaaacc ttagttatac tgtaataacc
     2161 gctgccgttg acggacagat atcgaaagtt aaccttcagc cgggtcagtt catacaggca
     2221 ggtcagtctt tgttcaatat tgtccccgac aaagagaaat gggttgtggc aaattttaaa
     2281 gaaacacaac tcgccaagat gaatatcggg cagaaagtaa gtat
//
LOCUS       DAOQQY010000373         2315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_26040_length_2315_cov_5.085841, whole genome shotgun sequence.
ACCESSION   DAOQQY010000373 DAOQQY010000000
VERSION     DAOQQY010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26040_length_2315_cov_5.085841"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1643)
                     /gene="rpoN"
                     /locus_tag="PLE23_12555"
     CDS             complement(96..1643)
                     /gene="rpoN"
                     /locus_tag="PLE23_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HMX84143.1"
                     /translation="MLKQSLSQKMLQKLSPQQIQLMKLLQIPTATLEQRIKEELEANP
                     ALDEGDEEDDFYGENSHNQDESTSKEENDDFTDTDDNEDNYSSEEYSLEEYLSEYMND
                     DDAGSYKLKGEGYHEEEEKQVPFAVENSFHEYLEAQLGLINLTDERETSIAKQIIGSI
                     DDDGYLRREPIAIIDDLMFSQNIITTEEEVMQVLKKVQTFDPPGVGARDLRECLLLQL
                     KHKVAILPSDTTALDKKVLEIAIRIIDQYFEEFSKKHYPKLQRTLGVSELELKYAIDE
                     ILKSNPKPASGYEESNSHSINYIIPDYIIVNREGNLELTLNSRNAPDLRINEHYVDML
                     KEYNINRQNQRATRKDKEAILFIKQKIDSAKWFIDAIRQRQHTMYTTMYAIMQFQAEY
                     FLTGDDTRLKPMILKDISDITGLDISTISRVANSKFVQTEFGTKRLKEFFSESLQTED
                     GSEVSTLEVKKILTDVIGGENKRKPLSDEKLKNILRNKGYNIARRTVAKYREQLNIPV
                     ARLRKEL"
     gene            1799..>2315
                     /locus_tag="PLE23_12560"
     CDS             1799..>2315
                     /locus_tag="PLE23_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HMX84144.1"
                     /translation="MAAKDHVLAIQEALKKSKIDAYIIPSSDPHQSEYVADHWKSREW
                     VSGFTGSAGLVVITQKEAGLWTDSRYFLQAETELKASGMVLHKQFIQGAPDHIEWLVN
                     TLDQGSMVAFDGKCFSIAQVDAMQKEFAKAGISIDSSIDFVAAAWKDRPLLPSGKFFE
                     LPEKYTGESRTE"
BASE COUNT          617 a          481 c          479 g          738 t
ORIGIN      
        1 ataatttcat tgaatgcctt caaaagtata aaagcttagt acgattaatt cacttttggc
       61 tcaacatgat gccgtcggag aatgtggtat tgcacttaca attcttttcg taaccttgct
      121 accggtatgt tgagctgctc gcggtatttg gctactgtgc gtcgtgcaat gttgtagcct
      181 ttgttgcgga ggatgttttt gagtttttca tcggagagtg gcttgcgttt gttttcgcca
      241 cctatcacat cggtgagtat tttctttact tcaagcgttg acacttcgct tccgtcttct
      301 gtctgtagtg attcggagaa aaattccttc aggcgtttgg taccgaactc tgtttgtaca
      361 aatttgctgt ttgccacacg cgaaatggtc gaaatatcga gacctgtgat gtctgatata
      421 tccttcagaa tcataggttt gagccttgtg tcatcgcccg tgaggaaata ttctgcctga
      481 aactgcataa tggcgtacat ggtagtgtac atggtgtgct gacgctgtct gatagcatca
      541 ataaaccact tggcagaatc aatcttttgt ttgataaaca agatagcttc cttgtctttt
      601 cgtgtagcac gttgattctg tctgttaata ttgtattcct tgagcatatc tacgtaatgc
      661 tcattgatgc gcagatcggg tgcgtttcgt gagttaagtg tcagctcaag attgccttcg
      721 cggttaacga taatataatc cggaataatg tagttgatgg aatgtgagtt agactcttcg
      781 tagccacttg caggcttcgg gttcgatttc aggatttcat caatagcata ttttagctcc
      841 agctccgaaa caccaagtgt acgctggagt tttggataat gttttttgga gaattcttca
      901 aagtactggt cgatgatacg tatggctatt tcgagtactt ttttgtcaag agctgttgta
      961 tctgagggca gaattgctac tttgtgtttt agttgcaaca aaagacattc gcgaagatct
     1021 ctggcaccaa cacccggtgg gtcaaaagtc tgaacttttt taagcacctg catcacttcc
     1081 tcttcggttg taataatgtt ttgagaaaac atcaggtcat caataatagc tataggctcg
     1141 cggcgaaggt agccatcgtc gtctatgctg cctatgattt gtttggcaat agatgtttcg
     1201 cgttcgtctg taagattaat cagacccagc tgtgcttcaa gatattcgtg aaaagaattt
     1261 tctactgcaa aaggtacttg tttttcttct tcttcatgat agccttcacc ttttagtttg
     1321 tacgagccgg catcatcatc attcatgtat tccgacaaat actcttcaag cgaatattcc
     1381 tctgatgagt aattatcttc attatcatcg gtgtcggtaa agtcatcatt ttcttcctta
     1441 gatgtagatt catcctggtt gtggctgttt tcaccataaa aatcatcctc ttcatcgcct
     1501 tcatccaagg caggattcgc ctccagttct tcctttattc tttgttctaa tgtagcagtc
     1561 ggtatctgca gcagtttcat taactgaatc tgctgaggag acaacttctg caacattttt
     1621 tgactcaaac tttgttttag cataatcctc tttctattta caaataacca tcaacaaaac
     1681 gcatttcggc accgacaggt ttcatttcat atctaatttt taataattcg tagttattgt
     1741 tagtacaata tcgcaaaact aagctaaatt taacccttca ttataaaatc aacaaaaaat
     1801 ggctgcaaaa gatcacgtcc tcgccataca agaggcatta aaaaaatcaa aaatagacgc
     1861 ttacattata ccttcaagcg acccacacca aagtgaatac gtcgcagatc attggaaatc
     1921 aagagaatgg gtttcgggct tcaccggttc agcaggcctg gtggtcatta cgcaaaaaga
     1981 agccggcctc tggacagaca gccggtattt tctacaagca gaaacagagc ttaaggcttc
     2041 gggtatggtt ttgcacaagc aatttataca aggagcaccc gaccacatcg aatggctcgt
     2101 caatacactt gatcaaggaa gtatggtagc atttgacggc aaatgtttct ccattgctca
     2161 ggtagatgcc atgcagaagg aattcgcaaa ggcaggtatc agtatcgaca gtagtataga
     2221 ctttgtagcc gcagcctgga aagaccgacc actattgccc tcaggaaaat tcttcgaact
     2281 accggaaaaa tataccggcg agtcgcgcac tgaac
//
LOCUS       DAOQQY010000374         2295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_26427_length_2295_cov_7.567411, whole genome shotgun sequence.
ACCESSION   DAOQQY010000374 DAOQQY010000000
VERSION     DAOQQY010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26427_length_2295_cov_7.567411"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /gene="nadD"
                     /locus_tag="PLE23_12565"
     CDS             <1..448
                     /gene="nadD"
                     /locus_tag="PLE23_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HMX84145.1"
                     /translation="LKDKSSLAPERDRLHMIRLSIDDNPKLRASDIEFKLPKPSYTID
                     TLTYLKEKYPKREFVLIMGSDNIETLQKWKNYERLLNDYNIYVYRRPGYDQNPFPDHS
                     GIQFYDAPQMMISASMIRQYIQEKKSVRYMVPEPALEYLESGHLYR"
     gene            573..>2295
                     /locus_tag="PLE23_12570"
     CDS             573..>2295
                     /locus_tag="PLE23_12570"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HMX84146.1"
                     /translation="MKIGEWKNFYPYRTGVSVTQSEAEVYYGNNLAIVVCDKNANIRR
                     YITRTTGLTDIDLGIIKYTPYKNTLIITYQNGTIDLYRNENDVTSLNYIKVNNNIVGK
                     KGINHIFLKNEKTALLSTDFGLLFMDIENEEFSESVFTPFPVYNTCVQNDTIYVATEG
                     GIYLIPPKINIQDFGQWQYLNANNNKPYKSTACAFYNGDLYFGLNDALVKQNEDKSFT
                     TLGTYKDNRVNYLTAEGKHLICGLFCTANGGCTGKILVVDDTNQITEHAGQSCFLRPL
                     YAVEDAANRVWIADEWDHYKYYDIDSDNCNYFTINSPNTETNFDILIANNKTYVTAGG
                     LILGQNYSFNGSGMNIYDHATKQWSVLNGDTYPELKQKEANFDFLNVVSDPVSPEKLF
                     IGSYYGGLIEYNGNGISIFNDQNSALSGGVGDALRERVAGLAYDSKGNLWMANTLAAN
                     PIVVKKKDGTWKSFPVNTNNALLQLAIDKNDNKWFIIGGQSGSIYVFNEGNDMNSTLD
                     DKWREINNSNSNLPSNLINTVSVDNRGDVWIGTAAGTVVFEYGGDIFNAAQKGTRPIV
                     EQDGFGAY"
BASE COUNT          766 a          479 c          435 g          615 t
ORIGIN      
        1 actaaaagat aaatcttcgc tggcacctga gcgtgaccgc ctgcacatga taaggctgtc
       61 tatagatgat aaccccaaac tacgtgcctc tgacattgaa ttcaaactgc ccaaaccttc
      121 ctacacaatt gacacactca cctacctaaa agagaaatac ccgaaaaggg agtttgtact
      181 cattatgggt agtgacaaca ttgaaacact ccaaaaatgg aaaaactatg aacgactgct
      241 caatgactac aatatatatg tgtatcgcag accgggctac gaccagaatc cgtttcctga
      301 tcattccggc atccaatttt atgatgcacc tcagatgatg atatcggcaa gtatgatacg
      361 ccaatacatc caggaaaaga agtctgtgag atacatggta cccgaacccg cactcgaata
      421 tttagaatcg ggacatctct accggtaaac aatgaaaacc acttattaat cgtttaatca
      481 gaaacattat ttactttatg cttcgcacat tgttacacat cgttttttta tgcatttgcg
      541 tcaataatct tgcatataat caagcttcgg atatgaagat aggtgagtgg aaaaactttt
      601 acccatacag aactggtgtg agcgtaaccc aaagtgaagc tgaagtatat tatggcaata
      661 acctagctat tgtagtttgt gataaaaatg ctaacattcg cagatatatc acaaggacaa
      721 ccggtcttac agatattgat ctgggcatta tcaaatatac accatacaaa aatacgctta
      781 tcataacata ccaaaatggc accatagacc tttataggaa tgaaaatgac gttacaagcc
      841 ttaactatat caaagtaaac aacaacattg taggtaaaaa aggtatcaat catattttct
      901 taaaaaatga aaaaaccgcc ttgctcagta cggactttgg tctgcttttt atggatattg
      961 aaaatgagga gttttcagaa agcgttttta ctccatttcc ggtctataat acatgtgtac
     1021 aaaacgatac tatatacgta gctaccgaag gaggtatata tctgattccg cctaaaataa
     1081 atatccagga ctttggtcaa tggcagtacc tcaatgccaa taacaacaaa ccatacaaat
     1141 ctacagcctg cgcattttat aacggtgact tgtatttcgg cctcaatgat gcccttgtaa
     1201 agcaaaatga agacaaaagc ttcactacac ttggtactta caaagacaac agagtgaatt
     1261 acctcacagc tgaaggcaaa cacctcattt gcggtctgtt ttgtactgcc aatggtggtt
     1321 gtactggcaa aatactagtg gtggatgata caaatcaaat aaccgaacac gctggccaga
     1381 gttgctttct ccgccccctg tatgccgtag aagatgctgc caaccgcgta tggatagcag
     1441 atgaatggga tcattacaaa tattacgata tcgacagtga caactgcaat tatttcacca
     1501 tcaactcacc gaataccgaa accaactttg acattctgat agcaaataat aagacctatg
     1561 ttactgccgg tggtctcata ttgggtcaaa attattcttt caacggtagt ggtatgaata
     1621 tctatgatca tgctacgaaa caatggagcg tactcaatgg cgacacctat cctgaactta
     1681 aacaaaaaga agcaaatttc gattttctga atgtcgtttc agatccggta agcccggaaa
     1741 aactattcat aggctcttac tatggcggac tgatagagta caatggcaac ggcatctcca
     1801 tttttaatga tcaaaattct gcccttagtg gtggtgtagg tgatgcactt cgtgaacgtg
     1861 tagccggact ggcttatgac tcaaaaggaa acctttggat ggccaataca ctagcagcta
     1921 acccgattgt agttaaaaag aaagatggca catggaaaag ctttcccgta aataccaaca
     1981 atgctctctt acaattggct attgacaaaa acgacaacaa atggttcatc attggtggtc
     2041 agtcaggtag tatttatgtg tttaatgaag gcaatgacat gaactctaca ctcgatgaca
     2101 aatggcgtga aataaacaat tctaactcca acctgccttc taaccttatc aatactgtat
     2161 cggttgataa ccgtggcgat gtgtggatag gtacagcagc cggcaccgtt gtattcgaat
     2221 atggaggaga tatcttcaat gctgcccaaa aaggtacaag gcctatcgtt gaacaagatg
     2281 gattcggtgc atacc
//
LOCUS       DAOQQY010000375         2290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_26531_length_2290_cov_17.551678, whole genome shotgun
            sequence.
ACCESSION   DAOQQY010000375 DAOQQY010000000
VERSION     DAOQQY010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26531_length_2290_cov_17.551678"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..714
                     /gene="tnpB"
                     /locus_tag="PLE23_12575"
     CDS             346..714
                     /gene="tnpB"
                     /locus_tag="PLE23_12575"
                     /inference="COORDINATES: protein motif:HMM:NF017528.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HMX84147.1"
                     /translation="MLGFGSHQRFFIYRQAIDMRKGVWSLCGLVRNDLQSNPLDGSVY
                     VFFSKSYQTVKLLVWDRDGFVVYMKRLERGRFESLSKIEGELKYSIGYQHLVMLLSGI
                     SLVGVKQKLRYNLNQTAIGA"
     gene            779..2266
                     /locus_tag="PLE23_12580"
     CDS             779..2266
                     /locus_tag="PLE23_12580"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HMX84148.1"
                     /translation="MNIEALIAKNLAQEKVIESQGLVIAKLQHQLDILLKQIYGRKSE
                     RFIPDTTNQPTLFSQVPQIEPEVQKETPREKEIVATYQRNKNHKGRRLTEGCEHLPIR
                     EEIIEIDCPEGFVKMGEVKSEKIGIDVLKLFLRVIIRTKYVNSQTGEILLAPMPEEAL
                     PKCEADETLLAHIAVSKFVDHLPEHRIQQMLKREQVAIPPSTMNTWTQKMAELFKLVA
                     MQIRKEILAGKYIQMDETTIKVLFVKKDKTHQGYMWVVVDPRTKNTYFEYREGRNRAG
                     PRLMLKDFQGKVQSDGYVVYDVIDAEFSDIDHYNCWTHARRKFIEALSDNKAVCEHTL
                     MLIQKLYEVEAGCRNDSCSLDERYERRQTYSLPILESLKEWMDQQALVVTPGCPTGKA
                     IAYCNRRWTNLIKYANTGNVEIDNNLVENAIRPLALGRKNYLFAGGHEAAVNIGYYYT
                     VFSTCKAQDVNPYDYALWYLRKINNTKITEIDTLTPSAYKKLVVN"
BASE COUNT          790 a          412 c          508 g          580 t
ORIGIN      
        1 attgtaagca tccgaccaac cacatattta taagccagat atgacacttt agctttgcct
       61 caaaaaagaa atgagcaaga gatattcaga agaggaaagg caaaggcatt tgaatgcatg
      121 cgagaagagc ggtttatcaa ttttggagta cagcaaaagg aacaatctga gtcctgggat
      181 catatccttt tggaggcgta gacagcaaag gctaagtgta agtaaatttg aagaggttgt
      241 tttgcctggt tcactcaccg gcgtgtttac aattgaatac cccactggaa taaaaattca
      301 tttggactca gccgtcgatc tctctgtaat aaaagctctt cttcattgtt agggtttgga
      361 tcacaccaaa gatttttcat ttaccgccag gccattgata tgcgcaaagg tgtctggagc
      421 ctttgtggcc tggtgcggaa tgacctgcaa agcaatcctc ttgacggatc cgtctatgtg
      481 ttcttttcaa agagctatca gacagttaaa ctattagtat gggatcgtga tggttttgta
      541 gtgtacatga agcgtcttga acggggcagg tttgaatcgc tgtcaaagat tgaaggggaa
      601 ttaaaataca gtatcgggta tcaacatctt gtaatgttat tgagcgggat atcgcttgtt
      661 ggggtaaaac aaaaattaag atacaatcta aatcagacag ctatcggagc ataaatttta
      721 ttgaaaaata tatgcacata tatatgaaat attgcatgtg tttttgtata tttataccgt
      781 gaatatcgaa gctctcatag cgaaaaattt agctcaagaa aaagtgatag aatcacaagg
      841 gcttgttata gcaaagctgc aacaccagtt agatattctg ttgaaacaaa tctatggtcg
      901 taaatctgaa aggtttattc ctgatactac aaaccagccg acattatttt cacaagtacc
      961 acagatagag cccgaagtac aaaaagaaac cccacgagag aaagaaattg ttgctacata
     1021 ccaacgcaac aaaaaccata aaggacgccg cttaacagaa ggatgtgaac atctacccat
     1081 aagagaggaa attattgaga tagactgccc agaaggcttc gtaaaaatgg gtgaagtgaa
     1141 atctgaaaaa ataggaatag atgtattgaa actgtttttg agggtaatca ttagaacaaa
     1201 atatgttaac tcacagacag gagaaatact attggcaccg atgccagaag aggctctgcc
     1261 taaatgtgaa gcggatgaaa cattattggc ccacatagca gtatccaagt tcgtagacca
     1321 tctaccagaa cataggatac aacagatgct aaagcgagaa caggtggcaa taccaccgtc
     1381 tacgatgaat acgtggacac agaaaatggc tgaactcttc aagttggtgg ccatgcaaat
     1441 acgcaaggaa atattagctg gcaaatacat acagatggat gaaacgacca ttaaagtatt
     1501 gtttgtaaaa aaagataaga ctcatcaagg atatatgtgg gttgttgtag atcctcggac
     1561 aaagaacacc tactttgaat acagggaggg tcgaaacaga gccggtcctc gacttatgct
     1621 aaaagacttc caaggaaaag tacaaagtga cggctacgta gtttatgatg ttattgatgc
     1681 agagttcagt gacatagatc actacaactg ttggacacat gcccgaagga aatttattga
     1741 ggcactctcg gataacaaag ctgtgtgtga gcatacatta atgctcatac aaaaactcta
     1801 tgaagttgaa gctgggtgtc gaaatgatag ttgtagtctt gatgaaagat acgaacgacg
     1861 tcaaacgtat agcttaccaa tattagaaag cctgaaagaa tggatggatc aacaagcttt
     1921 ggtagtcaca cccggttgcc ctacaggtaa ggcaatagcc tattgcaata ggaggtggac
     1981 aaacttgata aaatacgcca acaccggtaa tgtggagata gacaacaatc ttgtagagaa
     2041 tgcaatcaga ccacttgctt tgggtcgcaa aaactatctc tttgctggtg gtcacgaagc
     2101 agccgtaaac ataggttatt actacaccgt gtttagcacc tgcaaagcgc aagatgtgaa
     2161 tccatacgat tatgcactgt ggtacctccg aaaaataaac aacacaaaaa ttacggagat
     2221 tgacaccctc acacccagtg catacaaaaa attagtggta aattaggggt gtggttggtc
     2281 ggatgcttac
//
LOCUS       DAOQQY010000376         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_26679_length_2283_cov_5.835727, whole genome shotgun sequence.
ACCESSION   DAOQQY010000376 DAOQQY010000000
VERSION     DAOQQY010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26679_length_2283_cov_5.835727"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLE23_12585"
     CDS             complement(<1..525)
                     /locus_tag="PLE23_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84149.1"
                     /translation="MLGYRFTENIGESSSESTFDSLFKLFKELLLYTSGNIAEALEWL
                     TELDKEYNLTDDNYGIADFINELNERGYMLRQTDGNGGYIPSAKMEISIRKKAFGDLF
                     GNIKRYKPGRHTTRIAGRGDEHTSDIKPFEFGDKPENIAISESIRNAHVHFGIDDFML
                     SEDDLVLQETMYQSQ"
     gene            complement(739..1671)
                     /gene="mdh"
                     /locus_tag="PLE23_12590"
     CDS             complement(739..1671)
                     /gene="mdh"
                     /locus_tag="PLE23_12590"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMX84150.1"
                     /translation="MSKVTVVGAGNVGATCANVLAHKDFINEIVLIDIKSDMAVGKAL
                     DTWQQAPIDSYSTKLRGTEDYKDTADSDVVVITAGLPRKPGMTRDDLIATNAQIVASV
                     TENIVKYSKNAIIIIVSNPLDVMTYAALNVSGFPSSKVFGMAGILDTARYRAFLATEI
                     GVSPKDIQALLLGGHGDTMVPLPRYTTVAGIPVTDLVAKDKLDAIIERTQNGGGELVK
                     LMGTSAWYAPGAAAAQMVEAVLKDEKRVFPCCVRLDGEFGFSDVCLGVPVVLGRNGIE
                     RIIELELNNEEQTLLANSAKAVSEVIEVYKNMKL"
     gene            1963..>2283
                     /locus_tag="PLE23_12595"
     CDS             1963..>2283
                     /locus_tag="PLE23_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84151.1"
                     /translation="MKKIFITIFTLVSTVVMSTAQTSNFCGTTEFASIAARLKANKAL
                     LAKNPVQEREVKWVPMKFHIISKSDGTGAVQEHRVLDMLCGLNEFFAPLDIQFYIKDG
                     FNYFS"
BASE COUNT          681 a          485 c          425 g          692 t
ORIGIN      
        1 ctgactttgg tacatagttt cctgcagaac aagatcatct tcgctcaaca taaaatcatc
       61 gataccaaag tgaacatgtg cgttgcgtat gctttcagaa atggcaatat tttctggctt
      121 gtcgccaaat tcgaagggtt tgatatctga agtatgctca tctccccttc ctgcaatccg
      181 agtggtatgc ctacccggtt tgtaccgttt gatatttccg aataggtcgc caaatgcttt
      241 tttacggata gaaatttcca ttttcgcgct tgggatataa ccaccattgc cgtcagtctg
      301 cctcaacatg tatccgcgtt cgttcagttc atttatgaag tcggcaatac catagttgtc
      361 atctgtaaga ttatattcct tatcaagctc agtaagccat tcaagtgctt cggcaatgtt
      421 accggaagtg tatagcagta gttccttaaa taatttgaac aagctgtcaa acgtactttc
      481 ggagctgctt tcgccaatgt tttcagtaaa tctgtagcct aacatcttgt agcattattt
      541 tttttgtaaa taagcaaata acattcttgg ttgaaatagt aaaaaataga aaaccttctc
      601 atccaggcct tgaattaatc tatcaatgaa ggaatatata atcagaacgc aactgtacta
      661 cattggttca aaaaaatgac tggcaccgag atattcacat ccggtgccag tcgttatata
      721 ttatgagcaa tgattatatc agagcttcat atttttataa acttcgataa cttcagacac
      781 tgcttttgcg gaatttgcca gcagtgtttg ttcttcgttg ttcaactcga gttcgatgat
      841 gcgttcgata ccgttacggc ctagtacaac cggtaccccg aggcacacat cgctgaagcc
      901 aaattcgcca tccaacctta cacaacaagg gaataccctt ttttcatctt ttagtacggc
      961 ttcgaccatt tgagcggctg ctgcaccagg tgcgtaccaa gcagaagtac ccataagttt
     1021 tacgagttca ccacctccat tctgagttcg ttcgatgatt gcgtcaagtt tgtcttttgc
     1081 taccagatct gttacaggaa taccagcaac agtagtataa cgtggaagcg gaaccatagt
     1141 atcgccatga ccacctagca ataaagcctg aatgtcctta ggtgaaacac cgatttcggt
     1201 agcaaggaaa gctctgtaac gtgcagtatc gagtatgcct gccatgccaa agacctttga
     1261 tgaagggaac ccggaaacat tcaaagcggc ataagtcatc acgtcaagtg gattcgacac
     1321 tattattatg atagcatttt ttgaatattt tacaatattc tcggttacac tcgctacgat
     1381 ttgggcatta gtagcaataa ggtcatcacg tgtcattccc ggttttcttg ggagaccggc
     1441 agtgataact accacatccg aatcggcagt gtctttgtaa tcttctgtac ctctcagctt
     1501 ggtgctgtaa gaatcaatag gagcctgctg ccatgtatca agagccttgc ccacagccat
     1561 gtcgctttta atatctatca gtacgatttc attgataaaa tctttatgtg ctaaaacatt
     1621 tgcacaggta gcacctacgt ttccggcacc aacaacggtt actttactca taagtctgaa
     1681 atttatttgt taattatata ttgaaaagtg ccgcaaaggt acgaaatatt tcaccaacag
     1741 acatcatcat tctgaattaa agtagggttg actgaaacgc aaatcctgca ttaaaccaga
     1801 taaggcacta atctcttcaa agcaaaaaaa atcttataca accattagtt ggagtcaatg
     1861 actaaaaaaa taaggattta actaaattcg tacttgcaat tcgtaacatt tacattaatt
     1921 ttgaactaaa attcaggtat tttcaaatct caaacaatta aaatgaagaa aattttcata
     1981 acaatcttta cgcttgtttc cactgtagtc atgtcaacag cacagacttc aaatttctgt
     2041 ggtactacag aatttgcctc aatagccgca cgtctgaaag ctaataaagc attactcgcc
     2101 aaaaatccgg ttcaggaacg agaggtaaaa tgggttccga tgaagtttca catcatttct
     2161 aaatcggacg gtacaggagc tgttcaggaa caccgtgtac tcgatatgtt gtgtgggctg
     2221 aatgaatttt tcgctccgct cgatatacag ttctacataa aggatggttt taattacttc
     2281 agt
//
LOCUS       DAOQQY010000377         2262 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_27089_length_2262_cov_4.243770, whole genome shotgun sequence.
ACCESSION   DAOQQY010000377 DAOQQY010000000
VERSION     DAOQQY010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27089_length_2262_cov_4.243770"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="PLE23_12600"
     CDS             <1..715
                     /locus_tag="PLE23_12600"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX84152.1"
                     /translation="IWTGSDSLSTKGQSVEIFIDDLNKEGYYKVLVVLGNCQGKTYTD
                     SVYVSIDPAPDAIDDLISVGNSDSLYNVAFLNNDLATAGIFLEMGSVSSGSYSLNNDN
                     TLNYFPDKNFFGAATIGYKICLVDCPLACDSATIRIDVIDDKICIVPTLISPNGDDIN
                     DYLYIKCIDTGKYNDNRLVIYNEWGDQVFEAAPYQNNWKGTLEKVDGKNLPDGTYYFV
                     FFESREDKNPKYGFITILR"
     gene            736..1689
                     /locus_tag="PLE23_12605"
     CDS             736..1689
                     /locus_tag="PLE23_12605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HMX84153.1"
                     /translation="MMVKQIKIAILLLVCHLVATRVEAQQERHYTQFFVNKMTLNPAY
                     AGEDGIGSVTAIYRNQWLKFEGAPQSQLVTLNMPFSSRVGFGLGVYHSQIGLFETWEA
                     SMAYSYDLINRNNKSLRIGIQGSIRNFNIDVNNPSIITTDPSDPSVINVDGAKYYGNF
                     GMGLYLNINKFYFGVSTPRFYENVIGVNTITNLTAKEVAHYYLMTGLTIPASTNVDVQ
                     TNVLGKYVDGAPFNMDVNVNFIFFKALTLGAGYRTGGDKYGDAVEGIVQYLIGKKIGI
                     GVAYDYPLSQISGVSPGSIEGLLRLNFSNNKNNLKNPRFFF"
     gene            1722..>2262
                     /locus_tag="PLE23_12610"
     CDS             1722..>2262
                     /locus_tag="PLE23_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84154.1"
                     /translation="MKKIHIIYLLMLMSVSPMWAQTQDSIQGVVIQNENGINTSSLEF
                     SPAFHEDGIVYIVSQEQATGKKATDARLKKITMSIYEASRGADGKLTNPKPFSKELTS
                     KYHEGPVTFDRSGEKVYFTRNNLHKGKAKKSKDGFTHMRIYSADRVGGAWKNVQDLPI
                     NNDDFDCIHPSISVNGDKMY"
BASE COUNT          743 a          418 c          465 g          636 t
ORIGIN      
        1 catttggacc ggatcggaca gtttatcaac taaaggtcaa tctgttgaaa tattcataga
       61 tgatttgaac aaggaaggat attacaaagt attggtggta ctcggcaatt gccaaggtaa
      121 aacctatacc gattcagtgt atgtatccat tgatcctgct cctgatgcca ttgacgattt
      181 gatatctgta ggaaattcag attcgttgta caatgtagcg ttcctaaata atgatcttgc
      241 tactgcaggt atattccttg aaatgggcag tgtgtcatcc ggaagttatt cgctgaacaa
      301 cgacaacact ttgaattatt tccctgacaa aaatttcttt ggagcagcta ccattggtta
      361 caaaatatgt cttgttgatt gtccgttggc atgtgacagt gccaccatca gaatcgatgt
      421 gatagacgat aagatatgta tcgtaccaac actcatttca ccgaatggtg atgatataaa
      481 cgactatctg tacatcaaat gtatagacac aggcaaatac aacgataaca ggcttgtaat
      541 ctataatgaa tggggcgacc aggtattcga ggcagcacct taccaaaaca actggaaggg
      601 tacgcttgaa aaagttgatg gaaagaacct accagatgga acctactatt ttgtgttctt
      661 cgaatcaaga gaggacaaga accctaaata tggatttata accattttac gctaaaccca
      721 ataatgaaca acgtcatgat ggtaaaacaa ataaaaattg caatcttact ccttgtatgt
      781 catttggtgg caacaagagt agaagcacag caggaaaggc actatacaca gttttttgtg
      841 aacaaaatga ctctcaaccc cgcttatgca ggtgaagacg gaataggtag tgtaactgcc
      901 atatatcgaa atcaatggtt aaaatttgaa ggtgctccac aatcacaact agtgacactc
      961 aatatgccgt tctcttctcg tgtgggattc ggacttggtg tgtatcatag tcagattggg
     1021 ctttttgaga cctgggaagc ttcgatggct tattcttacg accttatcaa caggaataac
     1081 aagtctttga gaattggtat ccagggatca atcaggaatt tcaacattga tgttaacaat
     1141 ccgtcgatta ttacaaccga tccatcagac ccttcggtta taaatgtaga tggtgccaaa
     1201 tactatggaa actttggtat gggcttgtat ctgaatatca ataagtttta ttttggtgta
     1261 tcgacaccgc gattctatga gaatgtaatt ggtgtcaata ccataaccaa cctgactgcc
     1321 aaggaagtag cgcactatta tctgatgacc gggttgacta ttcctgcttc aaccaatgta
     1381 gatgtgcaga caaatgtact tggcaaatat gttgatgggg caccatttaa catggatgtc
     1441 aatgtcaact ttatcttttt caaagcccta acacttggag ccggttacag aacaggtggt
     1501 gacaaatatg gtgatgctgt tgaaggtatt gtacaatacc tgattggcaa aaagatagga
     1561 attggtgtag cgtatgatta tcctttgtcg caaatatcag gcgtatcacc gggcagtatt
     1621 gaaggattgt taaggcttaa tttttcaaat aataaaaata atcttaagaa tccaagattc
     1681 tttttctaag aaaccgataa tccaaaaaag cacacagctt catgaaaaaa atacacataa
     1741 tttatttgtt gatgcttatg tcggtatctc cgatgtgggc tcagacacag gattcaatac
     1801 agggtgtagt tattcagaat gaaaacggaa ttaacacatc cagccttgag ttcagcccgg
     1861 cattccatga agacggtatt gtatatattg tatctcagga gcaagccaca ggcaaaaaag
     1921 caactgatgc cagacttaaa aagataacga tgtctatcta tgaggcgagt agaggggctg
     1981 atggaaaact aaccaaccca aaaccattct caaaagaact gacttcaaaa tatcatgaag
     2041 gcccggttac atttgaccgc tccggtgaaa aagtatattt cacgagaaat aaccttcaca
     2101 aaggaaaagc aaaaaaatca aaagacgggt ttacgcacat gcgtatatac tctgctgatc
     2161 gtgtaggagg agcatggaag aatgtacaag atctacctat caacaatgat gactttgatt
     2221 gtattcaccc ttctatctct gtcaatggcg ataaaatgta tt
//
LOCUS       DAOQQY010000378         2252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_27275_length_2252_cov_6.280382, whole genome shotgun sequence.
ACCESSION   DAOQQY010000378 DAOQQY010000000
VERSION     DAOQQY010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27275_length_2252_cov_6.280382"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLE23_12615"
     CDS             complement(<1..273)
                     /locus_tag="PLE23_12615"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMX84155.1"
                     /translation="MAVFHEKLSRKFTFLKKENEMPVPAMTAEGIEIAPTVDFSNNKQ
                     DLAGKYGAGKAPYLRGPYASMYTQRPWTIRQYAGFSTAEDSNAFYRK"
     gene            514..1761
                     /locus_tag="PLE23_12620"
     CDS             514..1761
                     /locus_tag="PLE23_12620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMX84156.1"
                     /translation="MIRYRRFILNNGLRVVLHQDYTTPLAAINILYNVGSKDENPDKT
                     GFAHLFEHLMFGGSVNIRDFDTPLHHAGGENNAFTNGDITNFYDTVPVENLETVLWLE
                     SDRMISLAFNQKSLNVQKKVVVEEFKETCLNEPYGDAWHHLSDMNYKKHPYRWPTIGL
                     KPDHIEGATAQDVKDFFRKYYCPANAILVLAGPFEYEYALALVNKWFGDIKPGKANQR
                     NLPDETEQMKKERRVVCSHVPATSLYMTFRMPERMHPDFYAIDLLSDILGDGTSSRLY
                     DRLLKNQKLFSYIDGYITGSVEPGVFVIEGRPAEGVSVEEAEDAIWKELEILKCELVD
                     EDELLKLKRKLETSNAFSETSILNIAINLAYYEMLDEPDLINTEFDYYNAVTEQDIRK
                     VASDILQEHRCNTLVYLPEEKQR"
     gene            1825..>2252
                     /locus_tag="PLE23_12625"
     CDS             1825..>2252
                     /locus_tag="PLE23_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMX84157.1"
                     /translation="MTNHEYTNELIHESSPYLLQHAHNPVNWHAWKPETLAKAVAEDK
                     PILVSIGYSTCHWCHVMERESFEDPSVAALMNELFINIKVDREERPDIDHIYMEAVQF
                     ITGAGGWPLNCFLLPDGRPFYGGTYFPPEERYGRASWRQV"
BASE COUNT          704 a          438 c          475 g          635 t
ORIGIN      
        1 ttttctgtaa aatgcgttgc tgtcttctgc ggtagaaaaa cctgcatatt gtctgatcgt
       61 ccaagggcgt tgggtgtaca tgctggcata aggcccgcgc aagtacggag cctttccagc
      121 accatatttt ccggcaaggt cttgcttatt gttcgaaaaa tcaaccgttg gagctatttc
      181 aataccctca gcagtcattg ccggcaccgg catttcattt tctttcttta aaaaagtgaa
      241 tttcctgctt aatttttcgt gaaaaacagc catttaatta aaaattttag ataaagttac
      301 atcatttttc aggcaatagt agtggtagag tctgaaaatt aattctgaaa atatttagga
      361 gagacttatt tgaatcaatt ttttctgatg ttcagttgaa aatttgtatc tttgacaggt
      421 ggcaggcact aagacttgta gaatgtatat ttcaataaat tttaaaccat tctgtaagaa
      481 aatacattta aacatttaca aaaacgcaaa gatttgataa gataccgaag gtttattctt
      541 aacaacggcc tcagagtagt gctgcatcaa gattatacaa ctccactggc tgctatcaat
      601 atattatata atgtaggctc caaggatgaa aaccctgaca aaaccggttt tgcacacttg
      661 tttgagcatc tgatgtttgg tgggtctgtc aatatacgcg actttgatac gccactgcat
      721 catgcaggtg gcgaaaacaa cgcattcacc aatggtgata ttaccaattt ctatgataca
      781 gtaccagtag aaaatctaga gacggtttta tggcttgagt cggataggat gatttcgctc
      841 gcgtttaatc aaaaatcact caatgttcag aaaaaggttg ttgttgaaga atttaaagaa
      901 acttgcctca atgagccata cggcgatgcc tggcatcatc tttcggacat gaactataaa
      961 aaacacccgt acagatggcc aacaatcggt ctcaaaccag atcatataga aggtgcaact
     1021 gcccaagatg taaaagattt tttcagaaaa tattattgtc cggcaaatgc tatactcgta
     1081 ctggccggtc cgttcgaata tgaatatgct ttggctcttg taaataaatg gtttggcgac
     1141 attaagcctg gcaaagccaa tcagcgtaat ctgcctgacg aaacagaaca gatgaagaaa
     1201 gaaaggcgtg tagtatgctc acatgttccg gctacaagct tgtacatgac attccggatg
     1261 cctgaacgaa tgcacccaga tttttatgcc atagatctgt tgtctgatat tctgggagat
     1321 ggtacttcat ccaggttgta cgacagactg cttaaaaatc aaaaattgtt cagctacata
     1381 gatggctata ttacagggtc tgtagaacca ggtgtgtttg ttattgaagg tcggccggct
     1441 gaaggtgttt cggtagaaga ggcagaagat gccatctgga aagaattgga aatactgaaa
     1501 tgtgagctcg tagatgaaga cgaattatta aagctgaagc gaaaactcga aacctccaat
     1561 gcattctctg aaaccagtat cctgaatatt gccattaacc ttgcttacta cgagatgctc
     1621 gacgaacccg atttgattaa tactgaattc gattattata atgcagtgac cgaacaggat
     1681 atccggaaag tagctagtga tattctacaa gaacacaggt gcaatacact tgtatatttg
     1741 cctgaagaaa aacagcgtta gtaaacattt gtattacttt tataccattg taagcaagac
     1801 cggattccaa tttaacgaaa caacatgacc aatcacgaat ataccaacga gctcatacat
     1861 gagagcagcc cttatttgct ccagcatgca cacaatcctg tcaactggca tgcatggaaa
     1921 cccgaaacac ttgctaaggc agtagccgaa gacaaaccaa tacttgtgag catcggatac
     1981 tctacctgtc attggtgtca tgtgatggaa cgcgagtcat tcgaagaccc atccgtagca
     2041 gcattgatga acgaactgtt tatcaacatc aaggtggatc gggaagaacg accggatata
     2101 gaccatatat atatggaggc agtacagttt ataaccggtg cgggtggctg gcccttgaat
     2161 tgttttttat tgcccgatgg caggccattt tacggcggca cttattttcc accagaagaa
     2221 agatatggca gagcttcctg gagacaggta tt
//
LOCUS       DAOQQY010000379         2241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_27519_length_2241_cov_4.922690, whole genome shotgun sequence.
ACCESSION   DAOQQY010000379 DAOQQY010000000
VERSION     DAOQQY010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27519_length_2241_cov_4.922690"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="PLE23_12630"
     CDS             complement(<1..1166)
                     /locus_tag="PLE23_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020567378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMX84158.1"
                     /translation="MNKTRIITKSVWILAFVSMFTDMASEMLYPVMPVYLQSVGFSVM
                     LIGVLEGIAEALAGLSKAYFGRWSDVTGRRVPFVQVGYGLSAIAKPMMAILAVPLWIF
                     VARTLDRLGKGIRTGARDAILTEQSTNATRGRIFGFHRSFDTVGAVIGPLMALAYLYY
                     YPEDYRTLFLLAFIPGLLAIFASFRLKEGNTANGMQRKNTGFFSFIHYWKQSSAAYKK
                     LVIGLLIFALINSSDMFLLLKAKQSGLSDTMVIGVYILYNLVFAISAYPLGILGDRIG
                     LGRMFLLGVALFALVYLSIGFQTDVFVIVLLFLVYGIYIAATEGVSKAWISNFVGEKD
                     VATAIGTYTGFQSIATMFSSSIAGVIWYSYGSEVTFTVAGLGAVAVFVYFCLKV"
     gene            complement(1198..>2241)
                     /locus_tag="PLE23_12635"
     CDS             complement(1198..>2241)
                     /locus_tag="PLE23_12635"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX84159.1"
                     /translation="TSSRRGGMGGSDIWVTFKDANGNWKKPGPLAAPINDAGKSESPF
                     IHPDGRTLYFRSDRKPGIGGFDLYMAKMDEAGKWSVPLNLGYPINTSEDEGALIVSTD
                     GTTAYYSSDVKNSADIPNYGKNDIYRFTLPTEFRPGKVTYLKAKVIDDASGKAISANV
                     QLQRLKTGTSLTDMMVEDDGIFLVCIPAGEAYALNVSKPGYTFYSANFTMDTVTRTEP
                     FDMTISLQKITGNNVPGNKPVVLNNIFFNTGSASLRQESEYELNKLLAMLVENPSIKI
                     QINGHTDDVGNEADNLKLSTDRAKAVYDFLKAKGIDAARLSYKGFGEKMPLVENKDEK
                     SRQTNRRTEFEIK"
BASE COUNT          641 a          540 c          416 g          644 t
ORIGIN      
        1 ttcactttca aacaaaaata gacaaaaaca gccactgcac ctaaacccgc aacggtgaac
       61 gtcacttcag atccgtagct ataccaaata acccctgcaa tactactcga gaacatcgtt
      121 gcaatacttt gaaaacctgt gtacgtacct atagcggttg ctacgtcttt ttcacctaca
      181 aaattgctta tccatgcctt cgacacacct tcagtagctg caatgtagat accatacact
      241 aaaaaaagca agacaatgac aaatacatct gtctgaaacc cgatactcaa atatacaagc
      301 gcaaataatg ctacacctag caaaaacatc ctgcctaacc ctatgcggtc tcccagtatg
      361 cccaatggat aagcactgat ggcaaagacg agattgtaca aaatataaac accgatgacc
      421 atggtatcac tcaggccact ttgttttgcc ttgagcaaca gaaacatatc agaactatta
      481 atcaatgcaa atattaatag accgatgacg agctttttat aggctgcaga actttgcttc
      541 cagtaatgaa tgaatgaaaa gaagcccgta tttttacgct gcattccgtt agctgtatta
      601 ccttctttga gtcggaatga cgcaaatatc gcaagtaaac ccggaataaa agcaagcaga
      661 aagagtgttc tgtaatcttc aggataataa taaagatacg ccaaggccat caaagggcct
      721 atgacagccc ctacggtatc aaatgaacga tgaaatccaa atatacgccc tctggtggca
      781 ttcgtagatt gttctgtcag gatggcatcc cgtgcaccgg tccttatccc tttaccgagc
      841 cggtcaagtg tacgagcaac aaaaatccaa agcggcacag caagtattgc catcatgggc
      901 ttggcaatgg cactgagccc ataccctacc tgcacaaagg gtacacgtct gccggtaaca
      961 tcagaccacc ttccaaaata cgccttgcta agtccggcta aagcttcggc aatgccttca
     1021 agcacaccaa ttagcatcac cgaaaatcca acagactgca aataaaccgg catgacagga
     1081 tagagcattt cacttgccat atctgtgaac atcgacacaa atgccaaaat ccagacactc
     1141 ttcgtgataa tccgtgtttt gttcatcgta ttgccggaat gtttagtttg aacttactta
     1201 tttgatttca aattctgttc ttctgttagt ctgtcttgat ttctcatcct tgttttctac
     1261 caatggcatt ttttcgccaa atcctttata actcaacctt gccgcatcta ttccttttgc
     1321 tttaagaaaa tcataaactg ctttagctcg atctgtggat aacttcaggt tatctgcttc
     1381 attacctaca tcatctgtat gcccattgat ctgtatcttt attgacgggt tctccacaag
     1441 cattgctaac aatttgttca attcatactc cgattcttgt cgcagactgg cagagcctgt
     1501 attgaaaaat atgttgttga gtaccacagg cttattgccc ggcacattat tcccggttat
     1561 tttctgcaaa ctgatggtca tatcgaaagg ttctgtacgc gttacagtat ccattgtaaa
     1621 attggcggaa taaaaagtat agccgggttt tgatacattg agagcgtaag cttcacctgc
     1681 aggtatgcac accagaaata tcccgtcgtc ttctaccatc atatctgtga gacttgtacc
     1741 tgttttcagc cgttgcagtt gtacatttgc cgaaatagct ttgccggatg cgtcatctat
     1801 aacttttgct ttcagatatg taacttttcc cggcctgaat tctgttggca atgtaaaacg
     1861 gtaaatgtca ttcttaccgt aatttggaat atctgccgaa ttctttacat ctgatgagta
     1921 ataggctgta gtgccgtcgg tactaacaat aagcgcacct tcatcttcgc ttgtgttgat
     1981 cggatagccc agattgagag gaactgacca tttgcctgct tcatccattt ttgccatata
     2041 gaggtcgaag ccacctattc cgggtttgcg gtcgcttctg aaatacaatg tgcgtccatc
     2101 cggatgtata aatggtgatt ccgactttcc tgcatcattg ataggtgctg cgagtggacc
     2161 cggttttttc cagtttccat tagcatcctt aaatgttacc cagatatcag acccgcccat
     2221 gccaccacgt cggcttgatg t
//
LOCUS       DAOQQY010000380         2225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_27853_length_2225_cov_5.600461, whole genome shotgun sequence.
ACCESSION   DAOQQY010000380 DAOQQY010000000
VERSION     DAOQQY010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27853_length_2225_cov_5.600461"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..>2225
                     /locus_tag="PLE23_12640"
     CDS             119..>2225
                     /locus_tag="PLE23_12640"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HMX84160.1"
                     /translation="MRAQSVDCVSSGTQVDQIITDDCLLDDSERASLPIITMYVNVYF
                     IGSGGKNFTPTGANNTPNGIDWSNQMINKANELFSNLQDKNYYGYPSIVGDSRIRFEL
                     YSEEGNPNDPYGGIFFVDSLPITNFYPNKTINVLVQDDSIITDSTTLWGQQSGTKIWM
                     NNYYHYWTLNLPTFAGWNAAKLFLHEMGHYFTLCHSFYPYNVCKNIDIKVDWECNGTP
                     DNSMGCWAGKGECKLWESESHNIMSYSGQERDVLTPCQWKIMYRQATKSPLGVKECNS
                     EGDLLIISPGSYVEWLDAKIITNNVIVSSTATLRINCLTQFMSDVHIEVHRGGKLIVG
                     SEAILTNFCQGHRWGGIRVWGNATKEQPDPDSPMLSADDAGVVICKSGSKIMNANTGI
                     STIADLPWPLSKLYYGGVIKAVGASFINCRKGIEFMKYDFVNKSSIKSCKFTADANFI
                     KIAESSNKTGGVTIWDCDGIIFEANKFINLTDFGIMGIDFSCDITKGNIFDNVNIGVD
                     AKSSHEGGTQINIDGSVFTNIFSNNNNHIIVNNTTFQNGLTVLNNQFTDCGNVGISIA
                     GESQFKIQKNSFINNWFGISLSNTGVSVNNVSCNWLDNGCTGISAIGPNKSFTFFDNN
                     FSGYDYDFELKPGNIYKYQGAPGNPAENCFSKIKQIALGVFTTSMLVPNKSSFFLYYV
                     DENITDTTHCEYPKRKGKNN"
BASE COUNT          736 a          369 c          450 g          670 t
ORIGIN      
        1 tatatttgca gaagtagtcc aagtatttca ttcactaaaa attacagatt atgcaaacaa
       61 ttgtacacaa accatttgaa tatatatata tatattcata atttgttttt cagtaaactt
      121 gcgagcccag tcggttgatt gtgttagcag cggtacacaa gtcgatcaaa tcattactga
      181 tgattgcctg cttgatgatt ctgaacgggc gagtttgccc attataacaa tgtacgtaaa
      241 tgtttatttt ataggtagtg gtggaaagaa ttttacacct accggtgcta ataatacacc
      301 taatggcata gattggtcaa atcaaatgat aaataaagca aatgagttat tttcgaacct
      361 gcaagacaaa aactattatg gctacccttc catagttgga gattcaagga taaggtttga
      421 gttatatagc gaagaaggta acccaaatga cccttatggt ggtatttttt ttgttgatag
      481 cttaccgatt actaattttt atcctaacaa gacaataaac gtattggttc aagacgattc
      541 tataataaca gactcaacca ctctttgggg tcaacagagt gggacaaaaa tttggatgaa
      601 taattattat cattattgga cattaaactt acctactttt gcaggctgga atgccgccaa
      661 gctctttttg catgagatgg gtcattattt tacattatgt cattcatttt atccatataa
      721 tgtgtgtaag aacattgata ttaaagtaga ttgggaatgt aatggcacac cggataatag
      781 catgggttgt tgggctggta aaggagagtg caaactatgg gaatcagaat cacataatat
      841 tatgtcgtac agtggtcagg aacgagatgt attgacaccc tgccaatgga aaataatgta
      901 ccgtcaagca acgaagtcac cactcggtgt caaggaatgt aactctgaag gcgacctatt
      961 aatcatttcg cccggttctt atgttgaatg gttggatgcg aaaatcatca ccaacaatgt
     1021 aatcgtaagc tcgacagcaa cactcaggat caattgtctc acacagttta tgtcagatgt
     1081 gcatattgaa gtgcatcgag gagggaagct aattgtggga agcgaagcca tactgactaa
     1141 cttttgtcag ggtcacagat ggggtggcat aagggtgtgg ggcaatgcaa ccaaagaaca
     1201 acctgacccc gattcgccca tgttatcagc ggatgatgct ggggtggtta tctgcaagtc
     1261 aggctctaag attatgaatg ccaacaccgg catttctacc attgctgatt taccatggcc
     1321 actgagcaag ttatattatg gcggtgtcat taaagccgta ggtgcaagtt tcataaattg
     1381 caggaaagga atagaattca tgaagtatga ctttgtgaat aagagttcca taaaatcgtg
     1441 taaatttaca gcagacgcca atttcataaa aatagcagaa tcaagcaata aaaccggtgg
     1501 tgtaacaatc tgggactgtg atgggataat attcgaagct aacaagttta taaatttaac
     1561 tgattttggt atcatgggca ttgatttttc atgtgacatt acaaaaggaa atatattcga
     1621 taatgtaaac attggtgttg atgccaaatc aagccatgaa ggcggtacgc agatcaatat
     1681 tgatggatct gtgtttacaa atattttttc taataacaat aatcacataa tcgttaacaa
     1741 tacaacattc caaaatggtc ttaccgtgct taataaccaa tttactgact gtgggaatgt
     1801 cggaatcagc atagctggag agagtcaatt caagattcaa aagaacagct tcataaataa
     1861 ctggtttggc atttctcttt ccaatacagg agtttccgtt aataacgtat cttgtaattg
     1921 gctggataat ggctgtaccg gcatttctgc tattgggcct aataagtctt ttactttttt
     1981 tgataataat tttagtggat atgattatga ttttgaatta aaaccaggga atatatataa
     2041 atatcaaggc gcaccgggca accccgccga gaattgtttt tctaaaataa aacaaatagc
     2101 attaggtgta tttaccacaa gcatgcttgt gccgaataaa agttcttttt tcctatatta
     2161 tgttgatgaa aacataacag acactacgca ttgcgaatat ccaaaacgaa aaggaaaaaa
     2221 caatt
//
LOCUS       DAOQQY010000381         2220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_27961_length_2220_cov_5.400000, whole genome shotgun sequence.
ACCESSION   DAOQQY010000381 DAOQQY010000000
VERSION     DAOQQY010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27961_length_2220_cov_5.400000"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1800)
                     /locus_tag="PLE23_12645"
     CDS             complement(<1..1800)
                     /locus_tag="PLE23_12645"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HMX84161.1"
                     /translation="MNTSLKKGKAIRLTILALIMVLFMGVPGFCQNYDYAWLMGTKPK
                     VLPAELDTANNNFIIDFKNDSFKLIKSYETMTNMFLECNSLCDSSGTLLMYTNGCAVF
                     DATGNILPNGDSINYYVDGGNNAWVPSCGKGEKGYPLYRGTRFVPLFSHNRIFLLHTA
                     ASDEGNVFSYRYILYTEIEKKEGVWTVVNKNILLHDKPKVGVRYDITKHANGRDWWLV
                     AQNIGLHEYFVYLISPDGIKLQKTQNFPIPSAYTNTNPTFSPDGSKFFQYTPAAIKGD
                     SWLFDFDRITGTLSDPVIVHHPKSMFGQSSNAPFSPSGRFLYMCDVHHLYQVDLWDSD
                     TLTNRDTISVWDGLVWPCKVQCRTSYYTLQPGPDGKIYCGSDGASSYFFHVIERPNEK
                     GKACMFRPRAYRTPFYYHTNMPIYPYYRLGPIDGSAADTLGINNVPVAKFRYYPDSVD
                     WHKIEFVDNSYYNPTDWYWDFGDGSGNSSTEVNPIYSYAKSGVYDVCLTVSNVYGSDT
                     YCRYVRVGTVSTTEPNADRYIKLYPNPATNSINIELGEKLSGGHAYTIHCYDLLGRRC
                     YTGVLPAYSYLHTIDVSALAPGMYFIELTDDAGN"
     gene            complement(1811..>2220)
                     /locus_tag="PLE23_12650"
     CDS             complement(1811..>2220)
                     /locus_tag="PLE23_12650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84162.1"
                     /translation="TDSLYYVAEQCYGTGGRAVLDARHLLQILGLEASYDDDCYVEPR
                     SKEIQSQDKAESSVELIFNNPAAQILNYHLKGVDYKESELLIQNLTGDIVFYKKAPDQ
                     QGKIDIANIPNGFYIIIWKTGEQHFAEKLIIQQ"
BASE COUNT          662 a          489 c          393 g          676 t
ORIGIN      
        1 atttcctgca tcatctgtca gctctataaa gtacatgccc ggggcgagag ccgatacatc
       61 tatcgtgtgc aggtacgaat acgccggtag cacacccgta taacagcgcc tgcccagaag
      121 atcgtagcag tggatcgtgt aggcatgacc accggacaac ttttcaccca actctatatt
      181 gatgctgttc gtggcaggat tcggatatag cttaatatat cgatctgcat tcggctctgt
      241 ggtggacaca gtacctacac gcacatacct acagtatgtg tcgctgccat acacattgct
      301 caccgtcagg catacatcat acacaccact ttttgcatag ctgtatatag gatttacttc
      361 cgttgagctg ttaccgcttc catcaccaaa atcccagtac cagtctgtag gattatagta
      421 ggaattatct acaaactcta tcttatgcca atctacactg tcaggataat acctgaactt
      481 agcaactggt acattgttaa ttcctaaggt atcagcggca ctgccatcta tcggaccaag
      541 acggtaatac gggtagatgg gcatattagt atgataataa aaaggagtcc tataggcgcg
      601 tggcctgaac atacaggctt tgcccttttc attcgggcgc tctatgacat gaaaaaaata
      661 gctgctggca ccatcagatc cgcagtatat cttaccgtcc ggaccaggtt gcagggtata
      721 ataagatgta cggcactgca ccttacaagg ccagaccaga ccatcccata ccgatattgt
      781 atcccgatta gtcaatgtgt ctgaatccca tagatcaact tgatacaagt gatgtacatc
      841 acacatatat aaaaaccgcc cgcttggtga aaacggggca ttagaacttt gtccgaacat
      901 tgactttgga tgatgaacga taacaggatc gcttaatgta ccggtaattc tgtcaaaatc
      961 aaataaccaa gaatctcctt ttattgcagc tggagtatat tgaaaaaatt tacttccatc
     1021 cggtgaaaat gtaggattag tatttgtgta agctgaagga ataggaaaat tttgagtttt
     1081 ttgcagcttg atcccatccg gacttatcag gtaaacaaaa tactcatgca gtccaatatt
     1141 ttgagctact aaccaccaat ctcgaccatt cgcatgcttt gttatatcat agcggacacc
     1201 aacttttggt ttgtcatgta gaagaatatt cttattcact actgtccata caccttcttt
     1261 tttctcgatt tctgtatata atatgtatct gtaactaaaa acattgcctt catccgaagc
     1321 tgctgtatgc aataaaaaaa tcctgttatg agaaaaaagt ggtacaaagc gtgtgcctct
     1381 gtatagcgga tacccctttt ccccctttcc acaagatggt acccaagcat tgttccctcc
     1441 gtcaacatag taattaatag aatcgccatt gggaaggata ttgcctgtag catcaaaaac
     1501 agcacaaccg tttgtgtaca tcagaagcgt accacttgaa tcgcaaagag aattacattc
     1561 aagaaacata ttggtcatag tctcataact ttttattagt ttgaaactgt catttttaaa
     1621 gtctataata aaattattat tcgctgtatc cagttccgcc ggtaatactt tcggttttgt
     1681 ccccattaac caggcatagt cataattctg gcaaaacccg ggtacaccca taaataatac
     1741 cattattaaa gctaagattg tcaatcggat agcttttccc ttttttaatg aggtattcat
     1801 actatttatc ttattgttgg ataattaatt tttcagcaaa atgttgctca ccagttttcc
     1861 atattattat atagaaacca tttggaatat tcgcaatatc tattttgcct tgctgatccg
     1921 gagctttttt ataaaaaaca atatctcccg tcaaattctg tatcaataat tctgattctt
     1981 tgtaatcaac accttttaaa tgataattta aaatctgtgc tgccgggtta ttaaaaataa
     2041 gctctacaga agattctgcc ttatcctgtg attgaatttc tttagaccga ggctcaacat
     2101 agcaatcatc atcataagat gcttctaatc ccaatatctg aagtagatgc cgagcatcaa
     2161 gcacagctct tcctcctgta ccgtagcatt gttccgctac atagtacaaa ctgtctgttt
//
LOCUS       DAOQQY010000382         2196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_28496_length_2196_cov_5.011677, whole genome shotgun sequence.
ACCESSION   DAOQQY010000382 DAOQQY010000000
VERSION     DAOQQY010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28496_length_2196_cov_5.011677"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="PLE23_12655"
     CDS             <1..1774
                     /locus_tag="PLE23_12655"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HMX84163.1"
                     /translation="ASTKDGAALNGLKAVLEENERAKRYGFTPTELDRMKKQTETEYE
                     SAYKEQDKTMSRNLVSGIVSYFLDQTPFMNAAQEYELVKSLLPTITLEDVNACGRRFI
                     TDENRVVVVTSPEKPEIKLPTEAELLNLVNSAKGLRVEPYVDKVSDAPLMAQLPQKGS
                     VIKEEKNAELGTTTWTLSNNAVVVLKPTTFKNDEILFSATSEGGAFLYPDSEDLDASY
                     AAPIITGCGLGNFDNIQLDKYLTGKNVFVSPSIDNYREGMRGASSVADQETFFQLVNM
                     YFTQPRMDEVAFNSFKTKRISALKNFLSDPQRYFSVESNKIKTQDHPRMRFETAEEIE
                     KLNLKRMFDIYKERFSNAGDFTFFFTGSFTPESIKPFIEQYIASLPSNSGREKMKDLG
                     VRFPEGVVEKSWSKGDAPKSNVDITFHTPFEYTDRNRYVFSSLVDVLKIKLREELRED
                     KGGVYGVSVYGSTKNFPLAENTITISFNCTPGNEKNLIDATMKVLAKVKEIGANETDL
                     TKVKETQRQERIKNLEQNNFWSNTLLYAFQSKINPELILMKNYEPFINSLTADDIKSA
                     AKQYLDEKSMIKIVADPKPEVIKP"
     gene            complement(1853..>2196)
                     /locus_tag="PLE23_12660"
     CDS             complement(1853..>2196)
                     /locus_tag="PLE23_12660"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84164.1"
                     /translation="NGKLSILTALVDVNTTSVNEISNSEDQPVLSPNPATQTAYYSFK
                     LHRTEQVKVHLMNSEGKLITTLKSGSYPAGSYIEEIDTNKLQLQPGIYFIEKQLGDYT
                     KTSKLLITGSK"
BASE COUNT          702 a          411 c          488 g          595 t
ORIGIN      
        1 tgcttcaaca aaagacggtg ccgccctgaa tgggctgaag gctgtcctgg aagaaaatga
       61 aagagcaaaa cgctatggat ttacacctac tgagctcgat agaatgaaga aacagactga
      121 aacagaatat gaaagtgcat acaaggagca agacaaaacc atgtcaagga atctggtatc
      181 tggcatagtt agttattttc tcgaccagac acccttcatg aatgctgcac aagaatatga
      241 actggtaaag agtctgttgc ctaccatcac gctcgaggat gtgaacgcat gcggacgccg
      301 ttttataaca gatgagaaca gggtagtggt cgtaacatca ccggaaaagc ctgaaataaa
      361 acttccgaca gaagcagaac tgctcaacct tgtaaatagt gccaaaggcc ttagagtaga
      421 accttatgta gataaggttt cggatgcacc actcatggct caattgccac aaaagggttc
      481 tgtgatcaaa gaagaaaaaa atgctgaact tggtaccaca acctggacct tgagtaataa
      541 tgctgtcgtt gtacttaaac ctacgacatt caagaatgac gaaattttgt tctcggcaac
      601 gagtgaaggt ggcgcattcc tctatcctga ttcagaagac ctggatgcct cttatgcagc
      661 acctataatc actggttgtg gccttggcaa ttttgataat atacaattgg ataaatatct
      721 gacaggtaaa aatgtgtttg tgtcaccatc cattgacaat tatcgcgaag gtatgagagg
      781 ggcaagctcg gttgcagacc aagagacttt cttccagctt gttaatatgt attttactca
      841 gccaagaatg gatgaagtag ctttcaattc atttaagaca aagcggattt cggctttaaa
      901 gaatttcctc tctgacccac aaagatattt cagtgtagaa tctaacaaaa tcaaaacaca
      961 ggaccacccg agaatgagat ttgaaacagc cgaagagata gaaaagctca atctcaaacg
     1021 tatgttcgac atatacaaag aaaggttctc gaatgccggt gattttacat tttttttcac
     1081 aggatcattt actcctgaat ctataaagcc attcatcgag caatacatag caagccttcc
     1141 gtccaactca ggcagggaaa aaatgaagga tcttggtgtg cgttttccgg aaggtgtagt
     1201 agagaagtcc tggagtaagg gcgatgcccc gaaatcgaat gttgatatta cttttcacac
     1261 accatttgag tacactgacc gcaatcgtta tgttttttcc agccttgtgg atgtgctgaa
     1321 aataaagctc agagaagagc tgcgagagga caagggtggg gtatatggtg taagtgtata
     1381 cggatcaacc aaaaatttcc cgttggcgga aaacacgatt accatttcgt ttaactgtac
     1441 gccgggtaat gaaaaaaatc tgatagatgc taccatgaaa gttttagcca aggtaaaaga
     1501 aatcggagct aacgaaactg atctgacgaa ggtaaaagaa acacaacgtc aggaaaggat
     1561 aaagaacctt gagcagaata atttctggag caatactttg ctatatgcat tccaaagcaa
     1621 gatcaatcct gagttgatac tgatgaaaaa ctatgagcct ttcatcaata gtctgacagc
     1681 agatgatatc aaatcggcag ctaagcaata ccttgacgag aagagtatga taaaaatcgt
     1741 tgctgaccct aagccggagg tgattaagcc ttaagtaaac agatgtggta atattataaa
     1801 gatggctgct gatgtgatgt cgccagccat ctttttgtaa tagtgataaa gcttatttac
     1861 tacctgtaat aaggagttta ctggttttgg tataatcacc taattgtttt tctataaaat
     1921 agatacctgg ttgaagttgt agtttattgg tatctatctc ttctatatac gagccagccg
     1981 gatagctgcc acttttgagt gtggttatta gttttccttc actattcatc agatgaactt
     2041 ttacctgttc ggtacggtgg agtttgaagg agtaataagc tgtctgtgtt gctggattag
     2101 gtgaaagtac aggctggtct tcgctattgc ttatttcgtt aaccgaagta gtattcacat
     2161 caaccaacgc agtcaggatg ctgagcttgc cgttgt
//
LOCUS       DAOQQY010000383         2192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_28591_length_2192_cov_3.656996, whole genome shotgun sequence.
ACCESSION   DAOQQY010000383 DAOQQY010000000
VERSION     DAOQQY010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28591_length_2192_cov_3.656996"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2192)
                     /gene="dnaE"
                     /locus_tag="PLE23_12665"
     CDS             complement(<1..>2192)
                     /gene="dnaE"
                     /locus_tag="PLE23_12665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMX84165.1"
                     /translation="MAARFSDIPEALDNTLHVFDKIDTLKLKRDILLPKFPIPPGFDS
                     PTTYLRHLVYEGALHRYGELTAQVTERLDFELKIISDMGFEGYFLVVQDFIKAARKLG
                     VAVGPGRGSAAGSAVAYCLTITNIDPIRYRLLFERFLNPERISMPDIDIDFDDFGREK
                     VINYVVDKYGKNQVAQIITFGTMAAKSAIRDVARVLDLPLSDSDRIAKYLPNKPGFKF
                     KHILGKSLKEISDAFNSDDVANILKFNEISEQEKQGLETETVKRAIQLEGNIRNSGIH
                     AAGIIIAPDDITKYIPVCTAKDSDLLVTQFDGSVVESAGMLKMDFLGLKTLSIIADAI
                     TNITDRYGKDKHIDPDDIPLDDAATYELFQRGDTIGIFQFESDGMRKHLKDLKPTNIE
                     DLIAMNALFRPGPMDNIPSFINRKHGREKIEYPHEWLEDMLKPTYGIMVYQEQIMECA
                     RIMADFSLGKADSLRRAMGKKKAHEMEKNRKEFIEGAAKKNIPEEKSGEIFDIMEKFA
                     QYGFNRSHAAAYSVLAFQTAYLKAHYPAEFLASVLTHNKNNLETVNFFLKECKRLGIE
                     VLGPDINESKQDFTVNAAGQIRFGLAALKGVGEGPVAEILREREENGPFKDIFDMVER
                     LNLRNINKKCLESLVLGGGFDCFTDIDRPQYFASSGKYESFLEAIVKYGNTYKEQQQS
                     KVVSLFAEMEENFISKPEIPEAEAWPLIEKLEKEREMTGIYISGHPLD"
BASE COUNT          539 a          487 c          467 g          699 t
ORIGIN      
        1 catcgagtgg atgaccgcta atgtagattc cggtcatttc acgttctttt tccaactttt
       61 ctattaacgg ccaggcttct gcttccggta tttcgggttt ggagatgaaa ttttcctcca
      121 tttcagcaaa cagagaaacg actttgcttt gttgttgttc cttataggta ttaccgtatt
      181 tgacgattgc ctctagaaat gattcgtatt tgcccgacga tgcaaaatac tgtggccggt
      241 caatatcggt aaagcaatca aatccgccac ccaatacaag actttcgagg cattttttat
      301 tgatattgcg gagattgagc ctttctacca tgtcaaatat gtctttaaac ggaccatttt
      361 cttcacgttc tctgagaatt tctgctaccg ggccttcgcc tactcctttt aatgcagcaa
      421 gaccaaatcg gatctggcct gccgcattga ctgtaaaatc ctgcttactt tcattgatat
      481 ccggcccaag cacctctatg ccaaggcgtt tacactcttt caggaagaaa tttacggtct
      541 ctaggttgtt tttattgtgt gtaagcacag aggccaaaaa ctcagcaggg tagtgtgctt
      601 taaggtaagc tgtctgaaat gcgagtacag aataggctgc agcatgcgac ctattgaagc
      661 catactgggc aaatttttcc attatgtcga aaatttcgcc tgatttttcc tccggaatat
      721 ttttcttagc tgccccttca ataaattctt tgcggttttt ttccatttcg tgggccttct
      781 ttttacccat tgcccgcctg agcgaatctg ccttaccaag tgaaaaatcg gccattatcc
      841 ttgcacattc cattatctgc tcttgataaa ccatgattcc gtaagtgggt ttgagcatat
      901 cttcgagcca ttcgtgcgga tattctattt tttcacgtcc atgctttctg ttgataaaag
      961 aaggaatatt gtccatcggt cccggacgga aaagtgcgtt catagctatc aagtcctcaa
     1021 tgttggtcgg ttttaaatct ttgagatgct tgcgcattcc atccgattcg aactggaata
     1081 tgcctattgt gtctcctcgc tgaaataact cgtaggtggc agcatcatca agtggtatgt
     1141 catccggatc gatgtgtttg tccttcccat accggtcagt aatattagtg atagcatcgg
     1201 caatgatact gagtgttttt aacccgagga agtccatctt tagcatacct gcactctcta
     1261 ctacactgcc gtcaaactgt gtaacaagca ggtcagagtc cttggcagta cacaccggta
     1321 tatatttggt gatatcgtca ggtgcaatga tgatacctgc tgcatgaatg cccgaattgc
     1381 ggatattgcc ttcgagttga atggctcgtt ttacggtttc ggtttcaaga ccttgttttt
     1441 cttgttcact tatttcatta aatttgagaa tgttggctac atcatctgag ttgaatgcat
     1501 ctgatatttc ttttaatgat ttgcctaata tatgtttaaa tttgaagccg ggtttgtttg
     1561 gcagatattt ggctattctg tctgagtcag acaatggcag gtcgagcaca cgcgctacat
     1621 cacggatggc tgatttggca gccatagtac cgaaagtgat gatctgggct acttgatttt
     1681 tcccatattt gtcaaccaca tagttaatga ctttttcccg cccaaagtca tcaaagtcga
     1741 tgtctatatc gggcatcgat atcctttccg gattgaggaa acgctcaaag agcaatcggt
     1801 acctgatggg gtcgatgtta gtaatggtaa ggcaatatgc tactgctgac cctgctgccg
     1861 acccacgacc cggacctact gccacaccga gttttctggc tgccttgatg aaatcctgta
     1921 caaccaggaa gtaaccctca aagcccatgt cactaataat cttaagctcg aaatcgagcc
     1981 gttctgtaac ctgtgcagta agttcgccat atctgtggag tgcaccttca tataccagat
     2041 ggcgcaagta tgtggtaggg ctgtcaaaac ccggtggaat cggaaattta ggaagaagaa
     2101 tgtcgcgttt taacttcaga gtatctattt tgtcaaaaac gtggagtgta ttatcaagtg
     2161 cttcaggtat gtcgctgaac cttgctgcca tt
//
LOCUS       DAOQQY010000384         2186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_28723_length_2186_cov_7.432661, whole genome shotgun sequence.
ACCESSION   DAOQQY010000384 DAOQQY010000000
VERSION     DAOQQY010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28723_length_2186_cov_7.432661"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(192..2096)
                     /locus_tag="PLE23_12670"
     CDS             complement(192..2096)
                     /locus_tag="PLE23_12670"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HMX84166.1"
                     /translation="MKKIILLLSGLALCLSLQAQIADDQEYIKNFISNITVFTSGEIE
                     VSESIYINALGYEFKRGLVRRIPLKRKTQNGKSAKTPIEIVSIAHNGKKSEYHTKEEN
                     GYLAIYIGSADVFIDHGIHKYEIVYKTTNQIGFYNTYDEIYWNATGNEWAMPILQSNC
                     KVYLPRGAKIMQQACYTGAYGQQGYKCQMSDSGDEFVSFSSGELAPYEGLTVAVGFKK
                     AVVMPPPPPTWWETWRLRIIATLSFIVMAVYYVSTWQQYGRDPEAPVPFPQFTPPPGL
                     SLSEIGMINHENYSNRYLSATVLQWAVNGFVKITEDKDSYLFNLIQNTTYTVKKLKAP
                     DNSFKPHEARLFNSLFSGSDEFTIDGKYNASLYSAGEKFRSDTQSLYDHSEYGKGNNY
                     KYVAYGSLLLICFGLLMVFFDRDKIFIIATGVILCILTLVPFIVSSIFKSTQVIGVRL
                     YHQIAVWLLLIPTAVFIPLYIWPNILELDERVVVIFTILGIVSVLVYARLIKQPTSAK
                     LKLQSELEGFKMYLKMAENERVKLLNPPAETPELFEKYLPYAVLFKVEKVWGDRFREM
                     LSASPEHHGYTASWYSGNNFSGFTDSFSQNGFGSSFSSSSTQPSNYSSGSGGGGFSGG
                     GGGGGGGGGW"
BASE COUNT          615 a          453 c          456 g          662 t
ORIGIN      
        1 gtacaccaac cgagttgctc cggagtagtg gttgcccatt ccttacctaa gagtggtggg
       61 aaatacaatg actgagcgtg gaggaagccc gccatgaaga ataagataaa gatgagtttg
      121 gtctctttca tctgttggtt tattatttca gacaatgatt tgctgtaagg gtttaatatg
      181 tgtaaaaata tttaccaacc gccgccgccg ccgccgccac cgccgccacc ggaaaacccg
      241 ccaccaccac tgccggaact ataattagaa ggttgagtgg acgaactgct gaaggaagaa
      301 ccaaatccgt tctgtgaaaa actgtcggta aatccggaaa aattattgcc tgaataccat
      361 gaggcagtat atccgtgatg ttcaggtgaa gcagaaagca tttctctgaa acgatcgccc
      421 catacttttt ctactttaaa caatacggca tacggcagat atttttcgaa taattccggt
      481 gtttcggccg gtggattcaa tagcttgact cgttcatttt cggccatctt taggtacatt
      541 ttgaatcctt caagctcaga ctgtagtttg agtttggcac ttgtaggttg tttgataagt
      601 cttgcatata ccaatacaga aacgataccg agtatagtaa atataacgac taccctttca
      661 tccagttcaa gaatatttgg ccatatatac aaaggaataa atactgctgt aggtataagc
      721 agaagccata cagctatttg atggtaaagt cgtacaccta taacctgagt tgatttgaat
      781 atggaactta caataaaagg cacaagtgta agaatacaaa gtataacacc tgtagcaatt
      841 ataaatatct tgtcgcggtc aaaaaatacc atcagcaatc cgaaacagat caataacaat
      901 gagccgtatg ctacatattt gtagttgttg ccttttccat attccgaatg gtcatataaa
      961 gattgtgtgt cggatcggaa cttctctcct gcactatata atgaggcatt gtatttacca
     1021 tcaatagtaa attcatctga tccggaaaac aagctgttga acaatcttgc ttcgtgaggc
     1081 ttgaaactat tatccggtgc tttgagcttt ttgactgtgt aggttgtatt ttggatgaga
     1141 ttgaataaat aggaatcttt atcctcggtt attttaacaa aaccattcac tgcccattgg
     1201 agcacagttg ctgacaagta acggtttgag taattttcat gattaatcat cccgatttca
     1261 gacaggctca atccgggtgg tggtgtaaac tgtgggaaag gcaccggagc ctcagggtct
     1321 ctgccgtact gctgccaagt actcacataa tacacggcca taactataaa ggacaatgtg
     1381 gcgataatcc ttagacgcca ggtctcccac catgtaggtg ggggaggtgg catgacaaca
     1441 gcttttttaa atcctactgc gactgtcagg ccttcgtatg gtgccaactc acctgagctg
     1501 aaactgacaa attcgtcgcc gctgtcactc atctggcatt tgtacccttg ctggccatac
     1561 gcaccggtat aacacgcttg ctgcattatt tttgcgcccc tcggaagata caccttgcag
     1621 tttgactgta agatgggcat tgcccattca ttgccggttg cattccagta tatttcatca
     1681 taggtattat aaaatccgat ttggttagtg gttttataaa ctatttcgta cttgtgtatt
     1741 ccatggtcga taaacacatc cgcagagccg atgtatatgg ccaagtagcc gttttcttct
     1801 ttcgtatggt attcagactt tttgccattg tgggcaatgc ttactatttc aatgggagtt
     1861 tttgccgatt tgccattttg ggttttcctt ttcagcggaa tccgcctcac tagtccacgc
     1921 ttaaattcat atccaagcgc attgatgtag atactttcag atacttcgat ttcacctgaa
     1981 gtaaaaacag tgatatttga tataaaattt ttaatatact cctgatcgtc agctatctgt
     2041 gcctgcaaac tcaggcaaag tgctaagccg gagagtaaga gtattatttt tttcatgtca
     2101 gaatgaaatt tttggtacat ccctgtcaga tgtattttcg agttcgaaaa atgtagcctt
     2161 ctgaaactgg aactgattgg ctacca
//
LOCUS       DAOQQY010000385         2185 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_28750_length_2185_cov_6.174178, whole genome shotgun sequence.
ACCESSION   DAOQQY010000385 DAOQQY010000000
VERSION     DAOQQY010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28750_length_2185_cov_6.174178"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PLE23_12675"
     CDS             <1..501
                     /locus_tag="PLE23_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84167.1"
                     /translation="RMDINVMGTNITQAVNDKTGWAIVPFTGSSEPQPLSEEEVQELK
                     KQTELSRGILSWKDKGIKIESEGTEQLEGKDCFKLKFTDKDGKSSYKYFDKGTYYMVK
                     EIKYAEVEGQQMEIPVTYSDFRKTDSGLVFAYTMVNEMNGGPMKWESIEVNPKIDESV
                     FKIPTK"
     gene            754..1647
                     /locus_tag="PLE23_12680"
     CDS             754..1647
                     /locus_tag="PLE23_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMX84168.1"
                     /translation="MNRKDFIKAALVGAGLIVKSKAMGDIIKNDIDELTPLNPVKMNE
                     TIGFNHIPNTKSNIMENSVLHKANTRGHADHGWLNSHHTFSFANYYNPERMHFGVLRV
                     LNDDIIAAGTGFGTHPHDNMEIISIPLQGAIEHKDSMGNTSVIKSGDIQVMSAGTGIQ
                     HSEYNHSKTDAAKFLQIWVFPDKKNVTPRYDQISLNIADRKNKLQQILSPDSSDDSVW
                     IHQKAWFFLGNPEAGTTLDYRLKNHTNGVYVFVIKGNITIDGQQLSARDGYGIWSTET
                     IQIKATTDAEILVMDVPMSLS"
     gene            1816..>2185
                     /locus_tag="PLE23_12685"
     CDS             1816..>2185
                     /locus_tag="PLE23_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HMX84169.1"
                     /translation="MNIFQDTSRILISCQKMLGTALASEVKALGYETVNETPSVVEIQ
                     GTMDDCFILNLHLRTASQVLYHIRSFHAENAEELYQKARNIYWEDILLPDVYFSITGY
                     VNNETVNNSMYANVKLKDAIV"
BASE COUNT          750 a          398 c          455 g          582 t
ORIGIN      
        1 cgaatggata ttaatgtgat gggcacgaac atcactcagg ctgttaatga taaaacaggt
       61 tgggcaatcg taccttttac aggatcgtct gagccgcagc cattgtcaga agaagaagtg
      121 caggaattga aaaaacaaac tgaactgagc cgtggaatac tcagctggaa agataaggga
      181 atcaaaattg agtcagaagg aacagaacag cttgaaggca aagattgctt caaactaaaa
      241 tttacggaca aggacggaaa aagctcctat aagtatttcg acaaaggcac ttactacatg
      301 gtaaaggaaa ttaaatatgc cgaagttgaa ggacaacaaa tggagattcc ggttacttat
      361 tctgatttca gaaaaaccga ttcaggactt gtatttgctt atacaatggt caatgaaatg
      421 aacggcggcc caatgaaatg ggaatcaata gaggtgaatc ctaagataga tgaatctgta
      481 tttaagatac ctacaaaata attttacaca gccattgatc cgacaagcag tcaggaacaa
      541 cggtatgaaa taactaaatc attcaagcat acggcttctg aaattcattt cggggccgtt
      601 tttttttgga cgcctattcg gcgcaatttg ccgttatttc ttaattatct cgtattcagc
      661 ttattattaa actatcttaa gctgatttct taatgtagct tatggtcaga tggttttaaa
      721 tgcccgaact ttgcactatt gaatatatga atcatgaaca gaaaagattt tataaaagca
      781 gcattagtag gagcaggttt gattgtcaag tcaaaggcta tgggcgacat catcaaaaac
      841 gatatagacg aattgactcc tcttaatcct gttaagatga atgaaaccat agggtttaat
      901 catattccaa acactaaatc aaatatcatg gaaaacagcg ttttgcataa agcaaataca
      961 agaggtcatg cagaccacgg ctggcttaat tctcaccaca cattcagctt tgccaattat
     1021 tacaatcctg aaagaatgca ctttggggta cttagggtat tgaacgatga tattatagcg
     1081 gcaggtaccg gatttggcac ccacccgcat gataatatgg aaataatatc tattcccctg
     1141 caaggggcca tagaacacaa agacagtatg ggcaacacat ccgtaataaa aagcggtgat
     1201 atacaggtca tgagcgctgg tacaggcata caacacagcg aatataacca cagtaaaacc
     1261 gatgcggcaa agtttctgca aatatgggtt tttccggaca agaaaaatgt gacaccaaga
     1321 tacgaccaga taagcttgaa catagcagac agaaaaaata aactccaaca aatattgtcg
     1381 cccgactcaa gtgatgatag tgtctggatc caccagaaag cctggttttt cctgggtaat
     1441 ccggaggcag gcacaacact tgattataga ttaaagaacc atacaaacgg tgtttatgtc
     1501 tttgtcatca aaggcaatat aaccatcgac ggacaacaac tatcagcacg cgatggttat
     1561 ggtatctgga gtactgaaac aatccaaatc aaagccacca cagatgcaga aatactcgtt
     1621 atggacgtac cgatgagttt gtcgtaatat tattggagct taaagtttat cataatattt
     1681 caggattcag gatgtaaggc aactgccaaa cttattattg tattaagaag tatttgttgg
     1741 aggctaaaaa taatttgcat ctaagatatt gctttggtat tagttgtacc tttggtatcc
     1801 aataagatgg catatatgaa tatttttcaa gacacttccc gaatcctgat ttcttgtcag
     1861 aaaatgctgg gtactgcact ggcaagtgaa gtaaaggcct tgggttacga aacagtgaat
     1921 gaaacgcctt cggtagtaga aattcagggt acaatggacg actgtttcat actcaacctc
     1981 catttgcgca cagcgagcca ggtgttgtac cacattcgtt catttcatgc cgagaatgct
     2041 gaagagttgt accaaaaagc aagaaatatt tactgggaag atatattgct tcccgatgtt
     2101 tattttagca ttacaggcta tgtgaataat gaaactgtca acaacagcat gtatgccaat
     2161 gtaaagctga aggatgccat cgtag
//
LOCUS       DAOQQY010000386         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_28863_length_2179_cov_5.632298, whole genome shotgun sequence.
ACCESSION   DAOQQY010000386 DAOQQY010000000
VERSION     DAOQQY010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28863_length_2179_cov_5.632298"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLE23_12690"
     CDS             <1..511
                     /locus_tag="PLE23_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020569220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMX84170.1"
                     /translation="AIKFLTDAGIKRSRLKGVGYGEQQLKNGCKDGVTCTEYEHQLNR
                     RTEFRITKLKTGLNVQYLNNTPENVDAAPSMSSGSQPPVQVGIDPKEFEGANMLWVIG
                     GTFADKANAERRRSALSSKGFHEATIEAADNGTNQYVVVAKVSDAHKAVEIANELMSK
                     GFKTFIKKK"
     gene            710..>2179
                     /locus_tag="PLE23_12695"
     CDS             710..>2179
                     /locus_tag="PLE23_12695"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HMX84171.1"
                     /translation="MTDIEIAQKVQLKHISEIAAKLNLKADDLELYGKYKAKIPLHFI
                     NPENIKKNKLILMSAITPTPPGEGKTTMSIGLTQGMNRIGKKTTVVLREPSLGPIFGI
                     KGGAAGGGYSQVLPMEDINLHFTGDFAAIEKAHNLLSALVDNNIQNKKNNLGIDPRTI
                     TWKRVMDMNDRSLRKIVVGLGGTTGGVPREAGFDITAASEIMAIFCLSNDLPDLKRRL
                     GNIFVGFTYDKKPVYARDLKAEGAMAALLKDAIKPNLVQTIEGNPAIIHGGPFANIAQ
                     GTNSVIATKMGLSLSDYVVTEAGFGFDLGAEKFLNVKCQSAGLSPSAIVIVATVRALK
                     YLGGKDLKELTTPDPNALRNGLGNLEKHLENAKLYNIPAIVAINQFTTDSEEELNIIK
                     EKCASVGVQAIVANVWAKGGEGAEELARVVADAADNTTNKFTPLYSWDQPITEKIETI
                     CTKIYGADGVEYGSKAKTDMNRIKKLGLEGAPVIMAKTHK"
BASE COUNT          723 a          440 c          484 g          532 t
ORIGIN      
        1 tgctatcaag ttccttactg atgcaggtat taaacgaagc aggctcaaag gagttggata
       61 cggcgaacaa caattgaaaa atggttgtaa agatggcgta acatgtactg agtatgaaca
      121 tcagcttaac cgccgtacag agttcaggat aaccaaactg aaaacaggac ttaacgttca
      181 gtacctgaac aatacacctg aaaatgttga tgctgctcct tcaatgagtt ctggaagtca
      241 gcctcccgta caagttggca ttgaccctaa agaatttgaa ggtgcaaaca tgctatgggt
      301 aattggtggc acatttgccg ataaagccaa cgctgaaaga cgtcgttctg ctttatcatc
      361 caaaggcttt catgaagcta caatcgaagc ggctgacaat ggtacaaacc agtatgtagt
      421 ggttgccaaa gtatcggacg cgcataaagc tgttgaaatt gcaaatgaac tgatgagcaa
      481 aggattcaag acatttatta aaaagaaata attcatttca aagtttagat aagccggata
      541 atatgaattg tccggctttt ttattttatg atattccaaa ctgacgtaag gtaaatggtg
      601 taagtttata taaatttaac cgtgtaccta cattttggcc aagcagtttt gaaatccaaa
      661 caaattttca tatcattgca cggcattaat aacgataacc acatacatta tgactgacat
      721 agaaatcgca cagaaggtac agctcaaaca cattagtgaa attgctgcga aacttaatct
      781 taaagcagac gacctagagc tctacggaaa atataaagcc aaaataccac tacatttcat
      841 caaccctgaa aatataaaga agaataaact gatactgatg tcagctataa caccgacacc
      901 accgggtgaa ggtaagacta cgatgtctat cggcctgacg caagggatga acagaattgg
      961 caaaaaaact actgttgtac tacgagagcc ttcgctcggt cctatttttg gcattaaggg
     1021 tggtgctgca gggggcggtt attcgcaggt gcttccaatg gaagatatta accttcattt
     1081 tacgggtgac tttgcagcga tagaaaaggc tcacaacctg ttatcggctt tggttgacaa
     1141 taatatccag aataagaaaa acaacctcgg catagaccct cgtaccatta cctggaaacg
     1201 tgtgatggat atgaatgacc gatctttgcg caaaatagtg gtaggcttag gtggcaccac
     1261 cggcggtgta ccaagagaag caggatttga cataacggct gcttcagaaa taatggccat
     1321 tttctgtctg tcaaatgacc tgccggactt aaagagaaga ttgggaaata tttttgtcgg
     1381 attcacttat gataaaaagc cggtctatgc tcgtgacctc aaggcagaag gtgctatggc
     1441 agcattactg aaagatgcaa taaaacccaa tttggtgcag accatcgaag gtaacccggc
     1501 catcattcac ggaggcccat ttgccaacat tgctcaagga accaattcgg tcattgctac
     1561 taaaatgggt ttgtcacttt cagactatgt cgtaaccgag gcagggttcg ggtttgacct
     1621 tggagcagaa aagtttttga atgtaaaatg tcagtctgca ggtctcagcc caagtgcaat
     1681 agtgattgta gctacagtac gtgcactcaa atatctggga ggcaaagatc tgaaggagct
     1741 gacaacacct gacccaaatg cattgcgaaa tggccttggc aacctggaga aacacctcga
     1801 aaacgcaaaa ttatacaaca tacctgccat agtggccatc aaccagttta caacggacag
     1861 tgaagaagag ctcaacatca tcaaagaaaa atgtgcgtcg gtaggagtac aagccatcgt
     1921 agccaatgta tgggcaaagg gcggagaagg tgctgaagaa ctggctcgtg tagtagccga
     1981 tgctgctgat aacactacca acaaattcac tccactctat tcatgggatc agcctattac
     2041 tgaaaaaatc gaaactatct gtacaaaaat ctatggtgct gatggtgtgg aatatggttc
     2101 gaaagcaaaa accgacatga accggattaa aaaactaggc ctcgaaggtg caccagtcat
     2161 tatggccaaa acacacaag
//
LOCUS       DAOQQY010000387         2176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_28934_length_2176_cov_6.016502, whole genome shotgun sequence.
ACCESSION   DAOQQY010000387 DAOQQY010000000
VERSION     DAOQQY010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28934_length_2176_cov_6.016502"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLE23_12700"
     CDS             <1..629
                     /locus_tag="PLE23_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84172.1"
                     /translation="MFSKSWDEGATPQTRLKDWLDPLNTGKDTISGLEVPALPVCSIS
                     GKIETSKSASMKKVMVVLSGFTNDTVFTGNDGKYSFDKVVIGKNYTITPYYSTFALEG
                     VSTGDLVVINKSILGQQAIPSEYKKVAADVNLSGTISTADMNILNKMILGLQYEFPSK
                     KTWVFVTPGYKWPNQAGSAGSLHFNNIQVDQFNANFVGVKLGDVTGTN"
     gene            643..996
                     /gene="arsC"
                     /locus_tag="PLE23_12705"
     CDS             643..996
                     /gene="arsC"
                     /locus_tag="PLE23_12705"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HMX84173.1"
                     /translation="MQIFYNPRCGKCRNAKNFLEDQNISCDIFEYLSETFTEETLDEI
                     VRKSGLDVTDFIRKKEDLYKEKFAQKEYSHDEWLDILVENPSLLERPIIITEDRAWIA
                     RSDEKLQEVLQQYND"
     gene            1045..>2176
                     /locus_tag="PLE23_12710"
     CDS             1045..>2176
                     /locus_tag="PLE23_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84174.1"
                     /translation="MKRIHVVSFDVPYPPNYGGVFAVYQLLQFLKECNVNVVLHVFTY
                     NKRQISPELNVLCEKIITYKRNTGLWSQLSILPYIVRSRRHPDLRRNLIADDEPVLFE
                     GVHTCYWLKDVAKVKANVFVRAHNIEADYYRFLAKYESELTKRIFFSIEAYRLRFFEK
                     KILKLARKVFCFTQKDAEMLSHYGVRTVVSNPFFSSMRIQVNKGNGRGILIHGNLAIN
                     DTVQSIRLILQHFKDYPEIKMIIAGRNPADELINYLVGFPQVQLIINPDDAQLPELIQ
                     DAHIHLCHTNIEAGFKMRLMSLLQYGKFIVCNEKFCTEPLLKQALYIENDLAKWPALV
                     KTLLGKDFEPIDIEHRSNVVNTLNGTRGIGILIEEIFGKNTKS"
BASE COUNT          704 a          416 c          446 g          610 t
ORIGIN      
        1 gaatgttttc taaatcatgg gacgagggtg ccacaccaca gactcgtctc aaagactggc
       61 tggatccact caataccggc aaggatacca tctccggcct tgaagtgccg gcattgcctg
      121 tctgcagcat atcaggcaaa attgaaactt caaaatctgc ctctatgaag aaagtgatgg
      181 tcgtattatc cggattcaca aacgatacag tctttacagg caatgatggt aaatactcat
      241 ttgacaaagt agtcatcgga aagaattata ctattacacc atattatagt acctttgcac
      301 tggaaggcgt ttcaacagga gatctagtag ttatcaataa aagtattctt ggtcagcagg
      361 ccattccatc agaatataaa aaagtagctg cagatgtaaa tctgtccggt actatctcta
      421 cagctgatat gaacatactg aataaaatga tactcggttt acagtacgaa tttccttcca
      481 aaaaaacctg ggtttttgtg acaccgggat ataagtggcc gaatcaagcc ggcagtgcag
      541 gttccctgca cttcaataat attcaggttg accagttcaa tgccaatttt gttggcgtca
      601 aactgggcga tgtgaccgga actaattaat tatacaatag ctatgcagat attttataac
      661 ccacgatgcg gaaaatgtag aaatgctaag aactttcttg aagatcaaaa cataagctgt
      721 gacattttcg aatacctttc tgaaactttc acagaggaaa cactcgatga aatagtgcgc
      781 aaatctggcc ttgatgttac cgattttatt aggaaaaagg aagacctgta taaagaaaaa
      841 tttgcccaaa aggaatattc acacgatgag tggcttgaca tcctggtaga gaatccgtcg
      901 ttgcttgagc gaccgattat cattaccgaa gatcgtgcct ggatagccag gagcgatgaa
      961 aaactgcagg aagtgcttca acaatacaat gattaatcgc ttaattattg tacccggaat
     1021 tgtgcccaac tattatgtac tgttatgaag cgcattcatg tagtaagttt tgatgtgcct
     1081 tatccgccaa actatggtgg tgtgtttgct gtgtatcagt tgcttcaatt cctgaaagaa
     1141 tgcaacgtga atgtggtatt gcatgtgttt acatacaaca aaaggcagat aagcccggaa
     1201 ctcaatgtgc tttgtgaaaa aatcattacc tacaagcgca atacaggttt gtggtctcag
     1261 ttgtctatat tgccttatat tgtcagaagc agacgccacc cggacctccg acgaaatctc
     1321 attgccgacg atgaacctgt tctttttgaa ggtgtacata cctgctattg gctgaaggat
     1381 gttgccaaag taaaagccaa tgtctttgtc agagcacaca acatcgaggc agattactac
     1441 cggtttctag ctaaatatga atctgaactt acgaaaagaa ttttttttag catagaagca
     1501 tacagactcc ggttttttga aaagaagata ttaaaattag cccgaaaagt attctgtttt
     1561 acccaaaaag atgctgaaat gctgtcacat tatggcgtac gcacggtagt gtctaatccg
     1621 tttttttcat caatgcgcat acaggtgaat aaagggaatg gtcggggaat actcatccat
     1681 ggaaatctgg caatcaacga cactgttcaa tcaatcaggc ttattctgca acattttaaa
     1741 gattatcctg aaataaagat gatcatagca ggcagaaatc ctgcagatga attgatcaat
     1801 tatcttgtcg gatttcctca ggtacagctc ataataaatc cggatgatgc acaattaccc
     1861 gaattgattc aagacgccca tatacatctt tgtcatacca atattgaagc cggttttaag
     1921 atgcgattga tgtcattatt gcaatacgga aagtttatag tttgtaatga aaaattttgc
     1981 actgaaccat tattgaaaca agcgttgtac attgaaaatg atcttgcaaa atggccggca
     2041 cttgtaaaaa cactgctcgg caaagatttt gagccaatag atatcgagca taggtcaaat
     2101 gtggtgaata cactaaatgg cacacgtggt atcggaatac taatagaaga aattttcgga
     2161 aagaacacaa agtctg
//
LOCUS       DAOQQY010000388         2173 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_29015_length_2173_cov_5.065156, whole genome shotgun sequence.
ACCESSION   DAOQQY010000388 DAOQQY010000000
VERSION     DAOQQY010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29015_length_2173_cov_5.065156"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PLE23_12715"
     CDS             <1..1098
                     /locus_tag="PLE23_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX84175.1"
                     /translation="PNIVRATNGEINFMSDLTPKYGLENALYIQDDIKWNPRLSTNIG
                     LRFSSFSHIGPYVSSIDSTVYKDFEHIRTFYAVEPRVSAKYSVSPETSIKASLSGTTQ
                     YLHLVSNSTSTLPTDVWVPSSELVEPQRGIQYAAGVFRNFFSNTLETSIELYYKDMNH
                     QIDYGESYIASPADEVEKSFVYGIGKSIGAEFFIHKTTGRLNGWISYTLSKTRRKFDD
                     IAGGSWYPVTHDRTHDLNVVANYKINKKLELGAVFVFATGNTYTPLKNLFVTEQDLNI
                     EYGARNSARIQNYHRMDISLNYDPKPDSRKKFKSTWNFSVYNLYNRKNPAFIYNNIET
                     DVNKGTAKAQAIKVSLFPIIPSITWNFKWNQ"
     gene            1086..2033
                     /locus_tag="PLE23_12720"
     CDS             1086..2033
                     /locus_tag="PLE23_12720"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HMX84176.1"
                     /translation="MESIKMKQQSIFVLLILAITSLSSCEEEFIPEISSEPAQIVVEG
                     YIEAGEGALPPFVILTRSTPFFSEFSQDTWSKLFVHDAKVVVNDGVKDYVLNEICSDD
                     LTPEMKKNLSTLLGVDLDSSGIDFCAYLDLSFGLKGKEGGSYRLSVHADGQMLDAVTS
                     IPQRVGLDTVWFEQPPGEPSDTLRRMLCYITDPGDQTNYYRFLTKTNNQPFASGSNSV
                     VDDKLFNGIKFKVHLPRRSQRNEKFDPVSYGLYRVGDTAYVKWANIDKSHFDFWSTLE
                     FNKNNQGPFSSYTRTKTNINGGLGIWGGYYVNIYKLIVK"
BASE COUNT          678 a          469 c          436 g          590 t
ORIGIN      
        1 cctaatatcg ttcgtgccac gaatggagaa attaatttca tgtcagacct gactcctaag
       61 tacggacttg aaaatgcact gtatatacaa gatgatatta agtggaatcc gaggctgagc
      121 acgaacatcg ggcttcgctt ttcttctttt tcgcacatcg gaccttatgt ttcatccatt
      181 gattctacag tttataaaga tttcgagcac atcaggacat tctatgctgt agagcctcgt
      241 gtgtctgcca aatactcggt cagcccagaa acaagcatca aggcttcctt atccggaacg
      301 acacaatatc tgcacctggt aagcaattcg acaagcacac tgcctaccga tgtttgggta
      361 ccaagttctg agctggtaga accacagcga ggtattcaat atgctgcagg cgtgttccgc
      421 aattttttct ccaatacact tgagacctcc attgaactgt attacaaaga catgaatcac
      481 caaattgatt atggcgaaag ttacatagca tcacctgctg atgaagtcga aaaatcattt
      541 gtatatggca ttggcaaaag catcggtgcc gaatttttca tccacaaaac aacaggacgg
      601 ctcaacggat ggatttctta cacgctgagc aaaaccagga gaaaatttga cgacattgcc
      661 ggtggctcat ggtatccggt tacgcatgat agaacacatg acttgaatgt agtagccaac
      721 tacaaaatca ataaaaaact tgaactcgga gcagtatttg tttttgccac aggcaatacc
      781 tacactccgc tcaaaaacct gttcgtaacc gaacaagacc tcaatatcga atatggagcc
      841 cgcaatagtg cccgaataca gaactatcac cgtatggata tctctctgaa ttatgaccca
      901 aaacctgaca gccgtaaaaa atttaaatcg acctggaatt tttcggttta taatctttac
      961 aaccgcaaaa accctgcatt catatataac aatatcgaga ctgacgtcaa caagggtact
     1021 gctaaagccc aagcaataaa agtatctttg tttccgatta ttccttcaat cacatggaac
     1081 ttcaaatgga atcaataaaa atgaagcaac aatcaatatt cgtactactt atcctggcta
     1141 ttacttctct atcatcatgt gaagaagaat tcataccgga gattagcagt gaacctgccc
     1201 agattgtagt agaaggctac atcgaagccg gtgaaggcgc ccttcctccg ttcgtgatac
     1261 ttacccggag cacgcctttc ttttcagagt ttagtcagga tacctggagc aaacttttcg
     1321 ttcatgatgc taaggtagtg gtcaatgatg gtgtaaaaga ttatgttctg aacgaaatct
     1381 gctccgacga tctgacaccc gaaatgaaga aaaatctaag tactttgtta ggagttgatt
     1441 tggattcatc cggtattgat ttttgtgcct acctcgattt gagttttggt cttaaaggca
     1501 aagaaggagg cagctatagg ctttctgttc atgcagacgg acagatgtta gatgctgtta
     1561 caagcattcc acaaagggtt ggtctcgaca cggtttggtt tgagcagcca cccggagaac
     1621 cttcggatac acttcgcaga atgctttgct acataactga tcccggtgat cagacgaatt
     1681 attaccgttt tttgaccaaa accaacaacc aacccttcgc ttccggctca aactccgtag
     1741 tggacgacaa attgttcaac ggtatcaaat tcaaggttca tctaccacga cgcagtcagc
     1801 gaaatgaaaa atttgatccg gtcagttatg gtttgtacag ggtaggggat acggcttatg
     1861 taaaatgggc aaacatcgat aagtcacact tcgacttttg gagtacactt gaattcaata
     1921 aaaataacca ggggcctttc tcaagttata cacgtaccaa aaccaatatc aatggcggtt
     1981 taggtatttg gggtggatat tatgtaaata tttataaact aatagtaaaa taatggcaaa
     2041 taaaaatgta aaacgctact ctgacattct catcagtgtc gggcttgatg aagcaaatat
     2101 accggtagaa cttctttggc agtcaaaaga cgacccaaac gtcaaggaac ctcaggaatg
     2161 taaagctttt ctg
//
LOCUS       DAOQQY010000389         2167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_29185_length_2167_cov_4.571496, whole genome shotgun sequence.
ACCESSION   DAOQQY010000389 DAOQQY010000000
VERSION     DAOQQY010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29185_length_2167_cov_4.571496"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="PLE23_12725"
     CDS             <1..583
                     /locus_tag="PLE23_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HMX84177.1"
                     /translation="VGTQAAKMAAGLGARVTILDVNLNRLRHLADIMPANVTTIYSNE
                     YNIRQLIQDADLIVGAVLIPGAKAPHLITRDMLKLMRPGTVLVDVAVDQGGCIETCKP
                     TTHDNPTFIVDDIVHYCVANMPGAVPYTSTLALTNATLPYTLQLADKGWKKACQDNAV
                     LKKGLNIIKGDVVYKGVADAFGLPLHDVESFLN"
     gene            596..1834
                     /gene="rocD"
                     /locus_tag="PLE23_12730"
     CDS             596..1834
                     /gene="rocD"
                     /locus_tag="PLE23_12730"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HMX84178.1"
                     /translation="MSIKTAFSSSELIALEDKYGAHNYHPLPVVLSKAKGIYVWDVEG
                     KKYFDFLSAYSAINQGHCHQRIIDALIHQAQRLTLTSRAFYNDQLGIYEKYITDYFGY
                     DKVLPMNTGVEGGETAIKLCRKWAYKVKGIEENKAKIIFAAGNFWGRTLAAISSSTDP
                     TSYKDYGPYMPGFELVPYNDLEALSKALEDPNVAGFMVEPIQGEAGVVVPDANYLPDA
                     YRMCKEKNVLFIADEVQTGIARTGKILCCDHFGFKPDILILGKALSGGVLPVSAVLAN
                     DEIMMTIKPGEHGSTYGGNPLACAVAVEALAVVIDEQLAENADRMGAIFRHRMQKLIE
                     KTQLVTLVRGMGLLNAIVINDSEDSDTAWNICLKLAENGLLAKPTHGNIIRFAPPLII
                     NEEQMHQCCDIIEQTILSFS"
     gene            complement(1916..>2167)
                     /locus_tag="PLE23_12735"
     CDS             complement(1916..>2167)
                     /locus_tag="PLE23_12735"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HMX84179.1"
                     /translation="KDNRGSANMGCNNIMFGIKTKRCNKIEFTAPGATMMYCEDRMKL
                     EDEFGKLLPSVKKYKIEGHYLMLKAKNGTKMKFVAEDWD"
BASE COUNT          579 a          590 c          495 g          503 t
ORIGIN      
        1 cgtaggtaca caggctgcca aaatggctgc cgggctcggt gcccgtgtaa cgatactgga
       61 cgtcaacctc aaccgacttc gtcacctggc tgatataatg ccggccaatg tgacgaccat
      121 ctactctaat gagtacaaca ttcgccagct gatacaagat gccgacctga tagtaggagc
      181 cgtgctgata ccgggtgcca aagcacctca cctcataaca cgcgatatgc tcaaactcat
      241 gcgtccaggt acggtactgg ttgacgtggc cgttgaccag ggtggctgta tcgaaacctg
      301 caagccgacc acgcacgaca atccgacttt catcgtagat gacatcgtgc attattgtgt
      361 tgccaatatg ccgggtgctg taccttatac ttctaccctc gccctcacca atgctacgct
      421 gccatatacg ctgcagctgg ctgataaagg ttggaaaaaa gcctgccagg acaatgctgt
      481 acttaagaaa gggcttaata tcatcaaagg cgatgtggtc tataaaggtg tagcagatgc
      541 attcggactc ccactgcatg acgttgaatc atttttgaat taatatacta ctgatatgtc
      601 tataaaaaca gctttttcgt caagcgaact catagcgctc gaagataaat acggagcgca
      661 caactaccac ccgctgcctg tagtgctgtc aaaggccaaa ggaatatatg tgtgggacgt
      721 ggagggcaag aagtatttcg acttcctgtc ggcatattct gccattaacc aggggcactg
      781 ccaccagcgc atcatcgacg cactcataca ccaggctcag cggctcaccc ttacgtcaag
      841 ggcattctac aacgaccagc tcggtatcta tgaaaaatat ataaccgatt acttcggcta
      901 cgataaggtg ttgcctatga acacaggtgt agaaggaggc gaaaccgcca tcaaactctg
      961 tcgcaaatgg gcttacaagg taaaaggtat tgaagaaaac aaggctaaaa tcatcttcgc
     1021 agccggcaat ttctgggggc gcacactggc agccatctct tcgtcaaccg accctacaag
     1081 ctacaaagac tacgggccgt atatgccggg cttcgaactc gtaccgtaca acgacctcga
     1141 ggcattgtcc aaagcactcg aagacccgaa tgtggccgga ttcatggtcg agccaataca
     1201 gggcgaggcc ggtgtggtag taccagacgc caactatctg ccagatgcct accggatgtg
     1261 taaggagaaa aatgtactct tcatagccga cgaggtacag acgggtatag cgcgcaccgg
     1321 caagattctt tgctgcgacc acttcggctt caaacccgac atactcatac tcggcaaagc
     1381 gctctcaggt ggtgtactgc ctgtatctgc cgtacttgcc aacgacgaga tcatgatgac
     1441 cataaagccc ggcgaacacg gctctactta tggtggcaat ccactggctt gtgccgtagc
     1501 cgttgaagca ctcgccgtag tcatcgacga acaactcgcc gaaaatgccg accgaatggg
     1561 tgctatattc aggcatcgaa tgcagaagct catcgaaaaa acacaactcg taaccctcgt
     1621 gcgtggcatg gggctgctca acgccatcgt cattaatgac tccgaagaca gcgacaccgc
     1681 ctggaacatc tgcctcaaac tggccgaaaa cggactcctt gccaaaccaa cacacggcaa
     1741 tatcatacgg tttgcaccac cgctcatcat caacgaagag cagatgcacc agtgctgcga
     1801 catcatagaa caaacgatcc tctccttctc ttaaaggctg tgggtccatc aatacaaaac
     1861 ggagtgaatc agcatccgtg atccactccg ttttttttat cctgtttggg cagtattagt
     1921 cccaatcttc ggccacgaac ttcatctttg tgccgttttt ggctttcagc atcaggtagt
     1981 gcccctctat tttgtacttt ttgacagacg gcagcagttt gccgaattcg tcctcgagct
     2041 tcattctgtc ctcgcaatac atcatcgtag cacccggagc cgtaaactct attttgttgc
     2101 accttttagt cttaatgcca aacataatat tattacaacc catattagcc gagccacggt
     2161 tatcctt
//
LOCUS       DAOQQY010000390         2132 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_29981_length_2132_cov_5.806933, whole genome shotgun sequence.
ACCESSION   DAOQQY010000390 DAOQQY010000000
VERSION     DAOQQY010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29981_length_2132_cov_5.806933"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="PLE23_12740"
     CDS             complement(<1..1329)
                     /locus_tag="PLE23_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HMX84180.1"
                     /translation="MKKKIPLLQRDISWLRFNFRVLQEAMNPDVPLFERIKFLAIYSS
                     NLDEFYAIRVANARNLVKTGRKTIKELDFDPKDLLKQIRNIVANHHKHFSKTLNHEII
                     PQLKSHGIFLLKPEELNLTQRKFIDNYFNTELIPFAQPVLLADQLVKPFLQDSELYLA
                     ISLKNKSKKTNDIQYAIVKIPSDYLPRFISLPSKADRHDLILLDDIVRYSLKYIFPGF
                     EIIGSYSMKITRDAELYIDDEFKGNLLEKIRKSLVKRNIGPASRFVYDRSMPLNMLEY
                     FVNLFGIEPDDMIQEGKMHNNFDWFKFPDFGMKELKYKSMKQMLYPALTSGNLFEEIK
                     NKDHIIYTPYESYEPVIRFFEEAARDPDVTHIRITQYRVANESRIMHALSEAVKNGKE
                     VKVFIEVKARFDEAANLKWGEWLSAAGVKVQYSFPGLKVHSKMALVTRIEN"
     gene            complement(1521..>2132)
                     /locus_tag="PLE23_12745"
     CDS             complement(1521..>2132)
                     /locus_tag="PLE23_12745"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMX84181.1"
                     /translation="LDSYKIIGERLHKEYPDSDYDKTYKEMIAQISQAAQVQDAKEKI
                     KVGMEAPEISLPDPTGKNIKLSSLRGKVVLVDFWASWCGPCRRANPHVVSLYNKYKDK
                     GFTVYSISLDGVDERTKARISDNKALDAKMAEEKKKWLDAVAADQLAWPNHVSELKKW
                     DTSTAQEWGVDAIPRTFLIGKDGKIAAINAQGGSLEAEIQKLL"
BASE COUNT          568 a          407 c          402 g          755 t
ORIGIN      
        1 attttcaata cgtgtgacca aagccatttt gctgtgtacc ttcaaacccg gaaaactgta
       61 ctgtaccttt acaccggcag cgctgagcca ttctccccat ttcaggttgg cagcttcatc
      121 aaaccttgct tttacttcta taaatacttt tacttctttt ccgtttttga cagcctcaga
      181 gagtgcatgc attatacgac tctcgttcgc tactctgtat tgtgtgattc ttatgtgtgt
      241 aacatctggg tctcgggcag cttcttcaaa gaatctgata accggctcat acgattcgta
      301 tggagtgtat attatgtgat ctttgttttt aatttcttca aaaagattgc ctgaagtaag
      361 agcagggtag agcatttgct tcattgactt gtatttcaat tctttcattc caaaatcagg
      421 gaatttgaac cagtcgaagt tattgtgcat tttaccttcc tgaatcatgt catcgggttc
      481 aataccaaag aggtttacga agtattctaa catattcaga ggcatactcc tgtcgtaaac
      541 gaatcttgat gccgggccaa tattgcgttt taccagactt tttctgattt tctcaaggag
      601 attgccttta aattcatcat caatatataa ttcggcatct cttgttattt tcattgaata
      661 actcccaatt atttcaaaac ccggaaaaat gtactttaaa gaatatctga caatatcgtc
      721 cagcaaaatg aggtcgtgtc tgtctgcttt tgaaggtaag gaaataaaac gaggtaaata
      781 gtcagaagga atctttacaa tggcgtactg gatgtcattt gttttctttg atttgttttt
      841 caggctgatt gccaaatata attcagagtc ttgtaaaaaa ggttttacaa gttgatctgc
      901 cagtaacacc ggttgggcaa acgggatgag ttcagtattg aaataattat caataaattt
      961 tctttgtgta aggttaagct cctcgggttt taaaaggaaa ataccatgtg attttagctg
     1021 tggtatgatc tcatggttga gtgttttgct gaaatgtttg tgatggttag ctacaatgtt
     1081 cctgatttgt tttaataaat ctttaggatc aaaatcaagt tctttgattg ttttgcgtcc
     1141 ggtttttacc aaattacgtg cattggcaac tcgtatggca taaaactcat ctaggtttga
     1201 agaatagatt gccaggaatt tgattcgctc aaagagtggg acgtccggat tcatggcttc
     1261 ctggaggaca cgaaagttga atcttagcca acttatatcg cgttgaagta atggaatttt
     1321 tttcttcatt gtctataatt atgacaatat tcaatattac gtgataccat gttatctcct
     1381 gaattctatc agtaaagtta aatgaatttt aagaaaatac aaaatttgca aagcaatgat
     1441 tttgcttgta aatgctactc aaagagaaaa gcaccggttt gtagttccga tgctttatct
     1501 ttatatattt ttttacttta ttagagtagc ttctggattt ctgcctctaa gctaccacct
     1561 tgagcattaa tggccgcaat ttttccgtct ttgcctataa ggaacgttct cggaatggca
     1621 tctacacccc attcctgagc agttgaagtg tcccattttt tcaattctga tacgtggtta
     1681 ggccaagcaa gttggtcagc ggctacagca tcaagccatt ttttcttttc ttcagccatt
     1741 tttgcatcga gtgctttatt atctgatatt cttgctttgg ttctttcgtc aacaccatcc
     1801 agtgatatac tataaacggt aaaaccttta tccttgtatt tgttgtacaa gctcaccaca
     1861 tgaggatttg cccttctgca cggaccgcac caacttgccc agaaatcaac caataccacc
     1921 ttgccacgga gagaagaaag tttgatgttt tttccggtag ggtcaggaag agatatttcc
     1981 ggtgcttcca ttcctacttt tattttctct ttcgcgtctt gtacctgagc tgcctgtgag
     2041 atctgagcaa tcatctcttt gtaagtttta tcatagtcac tatccgggta ttctttgtgc
     2101 agtctttcac cgattatctt gtaagagtcg ag
//
LOCUS       DAOQQY010000391         2102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_30781_length_2102_cov_6.498779, whole genome shotgun sequence.
ACCESSION   DAOQQY010000391 DAOQQY010000000
VERSION     DAOQQY010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30781_length_2102_cov_6.498779"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="PLE23_12750"
     CDS             <1..1908
                     /locus_tag="PLE23_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMX84182.1"
                     /translation="SYNVFPESGFKIRNISFHTKDSVMLKYLPEINNTEKLIPGKRLN
                     SADYELEAIRLVRFFRNNGFASFNKTHISNLLIDTIGTSTDITIEILPPNQSTHHQQY
                     TINNITVFLDYTTANADEAPAMDTTINNVRLLSYTSPSPFKPAAIQKLIYINKAEIYN
                     QDDIDFTNKRLSSLPCFKFIKIKTEIDTEHQDKLNVIVLLTPADKYSSGIDYDLNWET
                     NTFLNQALIGTALSFNHKSKNIFKGGEVMNAAIEAGIAINPSLLNKNFINTLDLKISN
                     EFKLPKFNGFFGLYRALTRLPGKAHPIIGNKMFEDMQKLGTSSMLASYSYSRIVNQYD
                     IQALNLGYTINYQQSNLKKYQITHLGVSLLNTKLDPFFDSLISANTFLKRSLAERQLF
                     TGILFRELSFTYVKPSARWGEDWFGQINTELSGLEVWAVNSTVNLLRKSDVNWSRSLK
                     FAQYFKLESEGRYTKRYSKNLALATRLYSGIIIPFGFSSQTPYVKQFFSGGPNSLRAW
                     RIRELGPGNAQPPENVLTYYQTGNFKLEANAELRFPIFWYLKGAIFLDAGNVWDFEKS
                     TYGDQAVLKWNFYKQIALGTGVGIRADFDFFQIRFDAGLKLNDPGKWVFNPFKMRLSD
                     FNPNIAIGYPF"
     gene            complement(1910..>2102)
                     /locus_tag="PLE23_12755"
     CDS             complement(1910..>2102)
                     /locus_tag="PLE23_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HMX84183.1"
                     /translation="TNGKSVEQTERDLKALFPRATWNKLHLQIIFYGREYCPARGHDE
                     QKCMICSKYGKKEISKKVK"
BASE COUNT          636 a          508 c          374 g          584 t
ORIGIN      
        1 agttataatg tatttcctga aagtggcttt aagattcgca acattagctt tcatacaaaa
       61 gatagtgtaa tgttaaaata tctgcccgaa attaataata cagagaaatt gatacccggc
      121 aagcgactca actcagccga ttatgaactc gaagctattc ggctggtacg tttctttaga
      181 aataacggct tcgcaagttt caacaaaaca cacatctcca atctcctcat tgacaccatt
      241 ggcacaagca ccgatattac tatagaaata ttgccaccta accaatcaac acatcatcag
      301 caatacacca tcaataacat cacagtattc cttgattata ccactgctaa tgccgacgag
      361 gcacctgcga tggatacaac aatcaacaat gtccgtcttt tgtcttacac atcaccttcg
      421 ccattcaaac cggcagccat ccaaaaactc atttacatca ataaagcaga aatatacaat
      481 caggacgaca tcgattttac aaacaaaagg cttagctctc tcccttgctt caaatttata
      541 aaaataaaaa ccgaaatcga caccgaacat caggacaaat tgaacgtcat cgtactactc
      601 actccggctg ataaatattc atcaggcata gactacgacc ttaactggga aaccaatact
      661 ttcctcaatc aggctctcat cggcactgca ctcagcttca accataaaag taaaaacatc
      721 tttaaaggcg gagaggtcat gaatgctgcc attgaagcag gtattgctat caatccttct
      781 ttactcaata aaaatttcat caacacactc gaccttaaga tttccaatga attcaaattg
      841 ccaaaattta atggtttttt tggtctctac cgtgcattaa ccaggttgcc gggcaaggct
      901 catcctataa taggcaataa gatgttcgaa gatatgcaaa aactcggaac gagctcgatg
      961 ctggctagtt acagttatag tcgtatagtt aatcagtatg atatacaggc tcttaacctt
     1021 ggctatacga tcaattacca acaatcaaat ctaaagaaat atcagattac ccacctcggt
     1081 gtcagcttac tgaataccaa acttgaccca ttcttcgaca gcctaatatc cgcaaatact
     1141 ttcctcaaaa gatcacttgc cgaaagacag ttgtttaccg gaatcttgtt tcgcgaactt
     1201 tcctttacgt atgttaaacc ctcggcaagg tggggcgaag attggtttgg tcaaatcaat
     1261 actgaactat caggcctcga agtatgggca gtcaattcta ctgtcaactt actgcgcaag
     1321 tcagatgtca attggtcgcg gtccctgaag tttgcccaat acttcaaact cgaaagcgaa
     1381 ggtcgctata ccaagagata ttcaaaaaat ctggccctgg ctacacgttt gtattcgggt
     1441 attatcattc catttgggtt ttcttcgcag acaccttatg tcaaacagtt cttctctggc
     1501 ggacccaata gccttcgggc atggcgcatc cgtgagctgg gccccggcaa cgcacagcct
     1561 ccggaaaatg tccttactta ttaccagaca ggcaatttca aactcgaagc caatgccgaa
     1621 ctccgtttcc caatattctg gtatctcaaa ggagcaatat tcctggatgc cggcaacgtg
     1681 tgggactttg agaaatcaac ctacggagat caggctgtac tgaaatggaa cttttataaa
     1741 cagatagcac tgggcaccgg agtaggcatc cgtgccgact ttgatttttt ccagatcagg
     1801 ttcgatgccg ggcttaaact aaatgatccg ggaaagtggg ttttcaatcc cttcaagatg
     1861 cgtctgagtg acttcaaccc aaatattgct attggctatc ctttttagac tattttactt
     1921 ttttacttat ttccttctta ccgtatttcg agcaaatcat acatttctgc tcatcgtgcc
     1981 cccttgcagg gcaatactct cggccgtaaa aaataatctg cagatgtagc ttgttccatg
     2041 tagcgcgagg aaataatgct ttcagatccc gttctgtctg ctctaccgac tttccattgg
     2101 tc
//
LOCUS       DAOQQY010000392         2084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_31241_length_2084_cov_6.928043, whole genome shotgun sequence.
ACCESSION   DAOQQY010000392 DAOQQY010000000
VERSION     DAOQQY010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31241_length_2084_cov_6.928043"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..>2084
                     /locus_tag="PLE23_12760"
     CDS             21..>2084
                     /locus_tag="PLE23_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HMX84184.1"
                     /translation="MKSNLLLILLLCVANSVFSQQFSGEVKWGEKFRTEQSAGGKTII
                     IWPCTDCLYGDENPGLPFKSLTIPVPSFGKMSCHIISAKWEPVNIASEYTPLLQMPEN
                     YTLQSNVGGTRGKYSAFVSIPTVRKNASGSWERLLSYTLEATFTPTISVQTRNGWTTT
                     SALSTGDNYKVAVGADGIYKLDYNFFKNTLGIDPNTINPRHVKIFGNPGGMLPEASSI
                     DRPDDLIENAIYFSGEDDEKFDAGDYILFYASGANEKKYNEKTKLLNLVTNLYSAKNY
                     YFIQISGGNGKRISQSIYNANPAFWTNKFLDVKRVEDEKFNLLNSFEYAQGSGRRWFG
                     DAFSASTEKKYDSQFVFDDIILTDTVFMYAEFAARSSEFTKFTMTILGKQVNSSQISN
                     TDLGDVEAYYARYGNVNYKFLPTQSQINTSVKFIAGNNSTGWLDYIEMNAYRKLNMAS
                     SPLFFQDPNTLQYPEAGYEISNAKPNLWVWNISTPGNVVKENVTISNNTARFSRQVNG
                     LERFVSFYPDGNYNTPEYVSKIENQNLHGIEQTNMVILYEKSFEQQVMKLKSHRESKS
                     GLKISLVRIDQLYNEFSSGRQDISAIRDFSRMLYSRTPDYKYLLLFGDASFDYKNNLN
                     NQPHLNFIPVFETEESLNPVYSYPTDDFFGLLDPEEGKDLKGVIDISIGRMPVNNAEQ
                     AETAIN"
BASE COUNT          699 a          424 c          405 g          556 t
ORIGIN      
        1 tctacataat taataaaaaa atgaagtcta atctcttgct aatcctgctt ttgtgcgtag
       61 caaattcagt tttttcccag caatttagtg gtgaagttaa atggggagaa aaattcagaa
      121 ctgaacaatc tgccggagga aaaaccataa tcatttggcc gtgtacagat tgcctttatg
      181 gtgatgaaaa tccgggtctg ccttttaaaa gtctgaccat accggtaccg tcttttggta
      241 aaatgagttg tcatattatt tccgccaaat gggagccggt caatattgct tcagaataca
      301 ctccacttct ccaaatgccg gaaaattata ctttacagtc aaatgttggt ggtacaaggg
      361 gaaaatattc tgcctttgtc tccattccta cagtaagaaa aaatgcttcc ggatcatggg
      421 aacgcctgct tagttatacc ctggaggcaa catttacacc aaccatatct gtgcaaacca
      481 gaaatggatg gactacaaca tctgctttat caacaggcga taattacaaa gtagctgtcg
      541 gagcagacgg aatctacaaa ctcgactaca attttttcaa aaatacactg ggtatcgacc
      601 caaatacaat caatccacgc catgtaaaaa tattcggtaa cccaggcggc atgctgcctg
      661 aagccagcag tattgacaga cccgatgatt tgatcgaaaa tgccatttat ttttctggtg
      721 aagatgacga aaaatttgat gccggagact atattttatt ttatgcctca ggtgctaacg
      781 aaaaaaaata caacgagaaa acaaagctgc tcaacctcgt gaccaatctg tattcagcaa
      841 aaaattatta cttcatacaa ataagtggtg gcaatggtaa aagaataagc caaagcatct
      901 acaacgcaaa tcccgccttc tggactaata aatttctgga tgtaaaacgt gttgaagacg
      961 aaaaatttaa tctgctcaac agtttcgaat atgcccaggg ttctggcaga cgttggtttg
     1021 gggatgcttt tagtgcatct accgaaaaaa aatacgattc acaatttgta ttcgacgaca
     1081 tcatccttac agatacagtg ttcatgtatg ccgaatttgc tgccagaagt tcggaattta
     1141 caaaatttac aatgacaata cttggcaaac aagtcaattc ttcgcagata tcgaataccg
     1201 acttgggaga tgtagaggct tattatgcaa ggtatggaaa tgtgaactac aaattcctcc
     1261 ctacccaaag ccagataaac acttcagtca aattcatagc cggcaacaat tcgaccggtt
     1321 ggcttgatta catcgaaatg aatgcttacc gaaaactgaa catggcaagc agcccattgt
     1381 tttttcagga cccaaataca ctccaatatc cggaagcagg ctacgaaatc agcaacgcaa
     1441 agccaaatct ttgggtatgg aacatctcca caccgggcaa tgttgtaaaa gaaaatgtaa
     1501 caataagcaa taatactgca cgattttcga ggcaggtgaa tggattggaa aggttcgtgt
     1561 cattttaccc tgacggaaat tataacactc cggaatatgt gtcaaaaatt gaaaaccaga
     1621 atctacacgg aatagagcaa accaacatgg tcatattgta cgaaaaaagc ttcgaacaac
     1681 aagtcatgaa gctgaagtcg caccgtgaaa gcaaaagtgg ccttaaaata tcccttgtac
     1741 gtattgacca gttgtacaat gagttttcaa gtgggcgtca agacataagt gccatacgcg
     1801 atttctccag aatgttgtac tccagaacac cagactacaa gtatctttta ttgtttggag
     1861 atgcaagctt tgattataag aataatctca acaaccaacc tcacctcaat ttcattcctg
     1921 tgtttgaaac agaggagagc ctcaatccgg tgtattccta ccctactgat gattttttcg
     1981 ggcttcttga tcctgaagaa ggaaaggatt tgaaaggtgt tatagatatt tcaataggca
     2041 gaatgcctgt gaataatgct gaacaagcag aaactgccat caac
//
LOCUS       DAOQQY010000393         2043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_32361_length_2043_cov_4.923541, whole genome shotgun sequence.
ACCESSION   DAOQQY010000393 DAOQQY010000000
VERSION     DAOQQY010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32361_length_2043_cov_4.923541"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PLE23_12765"
     CDS             complement(<1..454)
                     /locus_tag="PLE23_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84185.1"
                     /translation="MQTYSETTQYIEKLETSLETNLDPAERIKLLSQLINSCLYTEIV
                     KTQYYLEEQKKLFVLVDNPVYYLQYLLHSAALENNLYNHNFAEAIYQRALALAESQLE
                     PVQIIEFYIDYVGTFLNQHNFAEASSYIAKTRKLLERYPSGRMEAHLLV"
     gene            complement(506..655)
                     /locus_tag="PLE23_12770"
     CDS             complement(506..655)
                     /locus_tag="PLE23_12770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HMX84186.1"
                     /translation="MKTKKQKTLAELKKDLELISKKELTQIVGGKKEKPKLTWTIKCG
                     GLIPQ"
     gene            complement(710..1471)
                     /locus_tag="PLE23_12775"
     CDS             complement(710..1471)
                     /locus_tag="PLE23_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMX84187.1"
                     /translation="MTKAVIIEDEKLGVTNLTNMLARYCPEVEVIGVAGSVTEAVDLL
                     NDPDCNPDVAFLDINLSDGLVFQVLNQLDDVNFEVIFVTAYEQFAIKACEYSAIGYIL
                     KPIDGEQLVAAVSRIKPGKLNKITERLQMFSQHYNVPNKFEKIGVAAIDGIYFVYLKD
                     IVRFEGEDNYTHIFLKNGEKITASKTIKAYEDMLAGANFFRVHKSHVINLNFIKKFVK
                     GDGGFLVMEDGKQIDVSRRRRPAFVEHMQKLQEEL"
     gene            complement(1612..>2043)
                     /locus_tag="PLE23_12780"
     CDS             complement(1612..>2043)
                     /locus_tag="PLE23_12780"
                     /inference="COORDINATES: protein motif:HMM:NF014168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMX84188.1"
                     /translation="IIKQQDKMKHWADLLGRVLLSAIFIFEAYDSIKYHHTTLEVMAA
                     YNITWRPVFLLNLSIFLLFAGGLMVLLGYRPKLGAFMLLLYWVPVTFIRHSFWNDPIS
                     IEREQSVHFMKNLAIIGGLIVVLVNGTGRFSIRKLFDTTKF"
BASE COUNT          582 a          390 c          400 g          671 t
ORIGIN      
        1 gtaccaacag atgcgcttcc atccgacctg aaggatagcg ttccagtagc tttcgagttt
       61 tggctatata agacgaagct tccgcgaaat tgtgttggtt gagaaatgta cctacataat
      121 cgatataaaa ttcaataatc tgtactggtt cgagctgtga ttcggccaaa gctaatgccc
      181 gttgatagat ggcctcggca aagttgtgat tatacagatt attttccagt gcggctgaat
      241 gaagcaaata ctggaggtaa tagacaggat tatctactag tacaaagagc tttttctgct
      301 cttcgagata atactgtgtc ttaactatct ccgtgtatag gcacgaatta atcaattggc
      361 tcagaagttt aattctctca gccgggtcga gattggtttc gagggatgtc tcaagtttct
      421 ctatgtattg tgtggtttca gagtatgttt gcatggcgaa gaagtgttat agcgtaatag
      481 ccggaaaaat atcctgcaaa tataattatt gaggaataag tcctccacac ttaatcgtcc
      541 aggttaattt tggtttttct tttttgcctc ctactatctg agtaagttcc tttttgctga
      601 tgagttcaag gtcttttttc agttcggcaa gggttttttg ctttttggtt ttcatggctg
      661 tagttttagg taaaatgtag gttttgtttt cagttggtat caatcggtat tagagttctt
      721 cctgtagttt ctgcatgtgt tctacaaatg ccggtcgtcg ccttctggat acatctattt
      781 gtttgccatc ttccattacc aaaaatccgc cgtcaccttt gacgaatttt ttaataaaat
      841 taaggttgat gacgtggctt ttatgcactc tgaaaaaatt ggcccctgcc agcatatctt
      901 cataggcttt aatggttttg gatgctgtaa ttttctcacc atttttcagg aaaatatgtg
      961 tataattgtc ttctccttca aatctgacga tgtccttcag atatacgaag tatatgccgt
     1021 cgatggctgc cacacctatt ttttcaaatt tattcggcac attgtaatgc tggctgaaca
     1081 tctgaaggcg ttctgtaatc ttattgagtt tgccgggttt gattctgctg actgctgcta
     1141 cgagttgctc accatcaatt ggctttaaga tataaccgat ggcactgtat tcacatgctt
     1201 taatggcgaa ttgttcgtag gctgttacga aaatgacttc aaaatttaca tcatcaagct
     1261 gattgagaac ctgaaaaacc aggccgtcgg aaagattaat atcaaggaat gcgacatcag
     1321 gattgcagtc cgggtcgttt agaaggtcaa ctgcttcggt aacagaacct gctacaccta
     1381 ttacctctac ttcagggcaa tatcgtgcca gcatgtttgt caggtttgtt acgcctaatt
     1441 tttcatcttc aattattaca gccttcgtca cttcacgggt attaatatgt ttgctaaata
     1501 ggtattttga aaaaacaaca agtctaaaac cttatcattc tattcgttat ggcaaaagta
     1561 agccaattat tgaaatttca acaatattaa agaaaaaaat tccgagtaaa attaaaattt
     1621 ggtcgtatca aacaatttgc ggatgctaaa ccgtcctgtt ccgttcacca gtaccactat
     1681 cagaccaccg ataatggcca ggtttttcat aaaatgaaca gactgttctc gctctatcga
     1741 tataggatca ttccaaaaag aatgcctgat gaaggttacc ggaacccaat ataatagcaa
     1801 cataaaggct ccgagttttg gtctgtaccc aagcaaaacc ataagcccac ctgcaaagag
     1861 taaaaaaatg gaaagattga gcaaaaatac cggcctccaa gtaatattgt aagcagccat
     1921 cacttcgagt gtagtgtggt gatatttgat agaatcgtaa gcttcaaaaa taaaaattgc
     1981 cgacaacaat actcttccta ataaatctgc ccagtgtttc attttatctt gttgttttat
     2041 aat
//
LOCUS       DAOQQY010000394         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_32600_length_2034_cov_4.581102, whole genome shotgun sequence.
ACCESSION   DAOQQY010000394 DAOQQY010000000
VERSION     DAOQQY010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32600_length_2034_cov_4.581102"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            33..1793
                     /locus_tag="PLE23_12785"
     CDS             33..1793
                     /locus_tag="PLE23_12785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMX84189.1"
                     /translation="MNEFEQLGLDTALVESITQMGYTTPTPIQKLVIPHVLSNPGDVV
                     ALAQTGTGKTAAFGLPLIQQLEPGLKTVQALVLSPTRELCVQIAENMQNFLPKGRKYN
                     VVAVYGGTNIMHQIKDIKRGADIIVATPGRLMDLIDRKAVDLSTIHHIVLDEADEMLN
                     MGFRDDIEHILGFTPDEKFVWLFSATFSSDIRKIASRYMNNPTELTANPSNEINQNID
                     HTYMVVEERFRFEALKRIVDFTPDIFGLIFCRTKLDAQEVAERMVKEGYVADSLHGDL
                     SQQQRDRVMAKFRNRTLKLLVATDVAARGIDVDDITHVIHYNQPDDIEYYTHRSGRTG
                     RQGKTGTSILIASPRDLGRITQLERFLKTRFRKLPVPTGIEVCEQQLLLLMEKVVNVE
                     VQEEQIAPYWGNVYAKLEHLDKEEIIKKFASVEFNRFLDYYKNAPDLNRKERERERSG
                     NFERKTPGIRTESGRRSGGKDNSETLYFNIGKKDRFDIGKMVGLLCNKGHVENRYLGK
                     IELRNDYCIVEVDSKYSHQLLEKFSTLKVNGKQIIARLSEEGIHSHNPPSKRHKAFSD
                     VKNAAKPDFKRKFKKSGGRY"
     gene            complement(1816..>2034)
                     /locus_tag="PLE23_12790"
     CDS             complement(1816..>2034)
                     /locus_tag="PLE23_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020535715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldC"
                     /protein_id="HMX84190.1"
                     /translation="SLFDSKVKDTLKIDLWTNELQVVEMDRFMFQTLRGLADTYMKAT
                     NNHKLANEMQQFVTYFGEQTEIIPKEKN"
BASE COUNT          653 a          420 c          473 g          488 t
ORIGIN      
        1 tatccacatc cgtgtagttg ctgatcgtag acatgaatga atttgaacaa ttagggctgg
       61 atactgccct tgtagagtcg ataacccaga tgggttatac cacacctacc ccaatccaga
      121 agttagtaat accgcatgtg ttgagtaatc cgggtgatgt agtagcactt gcccaaaccg
      181 gaaccggcaa aactgctgct tttggtctgc cgcttataca acaactcgag ccgggcctca
      241 agaccgtgca ggcacttgta ttgagcccaa cccgcgaact atgtgtacag atagctgaaa
      301 atatgcagaa ttttctacca aaagggagaa aatataatgt cgtagccgta tatggcggta
      361 cgaacatcat gcatcagata aaagatataa aacgaggtgc agacataata gtagcaacac
      421 caggcaggct gatggacttg atagacagaa aggccgttga cctgtcaact atacatcaca
      481 ttgtactgga tgaagcagac gaaatgctca atatgggctt cagagatgat atagaacaca
      541 tattgggatt tacaccggat gagaaatttg tctggttgtt ttctgccact ttctcttctg
      601 acatcaggaa gatagcaagc agatacatga acaatccgac ggagctaacg gctaacccat
      661 ccaatgaaat aaatcagaac atcgaccata cctacatggt agtcgaagag cggttcaggt
      721 tcgaagcgct taagcgcatc gttgatttta caccggatat attcggcctc attttctgcc
      781 ggaccaagct ggatgcacaa gaagtagccg aacgcatggt gaaggaaggt tacgtagctg
      841 acagtctgca tggtgacctt agccagcagc agcgcgaccg ggtgatggcg aagtttagaa
      901 accgaacgct taaactcttg gtagcaactg atgtagctgc tcgtgggata gacgtggacg
      961 acatcacgca cgttatccac tacaatcaac cggatgatat tgaatactac acacaccgca
     1021 gcggaagaac aggcagacaa ggtaaaacag gtacatctat actcatagca tctccacgag
     1081 atctgggccg gataactcag ctggagcgtt tcttgaagac ccgattcaga aaattgcctg
     1141 tacctacagg gatagaagta tgcgagcagc agttgctttt attgatggaa aaggtagtaa
     1201 atgtagaagt acaagaggag caaatagcac cttattgggg taatgtatat gccaaactcg
     1261 aacaccttga caaggaagag atcatcaaga aattcgcttc ggtagaattc aaccgttttc
     1321 ttgattatta caaaaatgca cctgacctca accgaaaaga aagagaaaga gaacgttcgg
     1381 gtaattttga acgaaaaaca ccgggcatcc gaaccgaatc gggacgaaga tccggaggca
     1441 aagacaacag cgagacattg tatttcaaca ttggtaaaaa agataggttc gatatcggta
     1501 aaatggtagg tttgttatgc aataagggac atgtagaaaa ccgatattta ggaaagattg
     1561 aactacgtaa cgactattgt atagtagagg ttgatagcaa atattcgcac cagctacttg
     1621 aaaaattcag tacactcaag gtaaacggca agcagataat agccagactt tcggaagaag
     1681 gcatacattc gcacaatcct ccatcaaagc ggcataaggc attcagcgat gtgaagaacg
     1741 ctgcaaaacc agacttcaag cgaaaattca agaagtcggg cggccgctat tgatgaaaga
     1801 gtacagaaat gagctttaat tcttttcctt aggtattatt tctgtttgtt cgccaaaata
     1861 agtaacgaac tgctgcatct cgttggcgag tttgtggtta ttggttgctt tcatataggt
     1921 gtctgcaagc ccgcgtagtg tctgaaacat gaacctgtcc atctccacta cttgtagttc
     1981 attggtccac aggtcgattt ttaaagtatc ctttactttc gaatcaaaca gaga
//
LOCUS       DAOQQY010000395         2027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_32783_length_2027_cov_5.486308, whole genome shotgun sequence.
ACCESSION   DAOQQY010000395 DAOQQY010000000
VERSION     DAOQQY010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32783_length_2027_cov_5.486308"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1116)
                     /locus_tag="PLE23_12795"
     CDS             complement(199..1116)
                     /locus_tag="PLE23_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020568299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMX84191.1"
                     /translation="MKKVLITGGAGFIPSSLAEHLLSTGRYHVTAIDNMLTGRQEHIL
                     QHPNYKFIKADANDYADISAIMLYNDFDYVFHYAAVVGVLRTLEHPKLVLDDIKGLQN
                     IFELSKNTDVERIFYSSSSEVYGEPVHLPQNEHVTPLNSRLPYAVLKNIGECFCRSYH
                     QEFGLNYTILRFFNTYGPRQSRDFVVSKFLDAARNNQDITVYGDGSQTRTFCYIQDNL
                     DFTLKILEDKLYINDVVNVGNDKIVTINELAESIIQLTGSKSKIVSLPPLKEGDMSRR
                     QPDIEKMKMVLNRELTPLSEGLMKVISAY"
     gene            complement(1119..>2027)
                     /locus_tag="PLE23_12800"
     CDS             complement(1119..>2027)
                     /locus_tag="PLE23_12800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX84192.1"
                     /translation="AQYINRHIPLKKFGQIVETHIGIESWEKKGNRELALSLKDFAPQ
                     LVLVFCNTKVLYGTLAFLRSVTKAKFGLLWPDTLFNLTPKVAANSKLFDFVGTYSSTS
                     VAVFEQAGFNNVFWLPLAADSFQHNKENLPDKYSSDISFIGGWRPEREQYLKLIRENF
                     QNADMLIKGPEWLKRCKIKSLRSIIDDGPAYGNAFAELLNSSRINLNIIDDTNYPAAN
                     MRFFEIPVSKGLQLSSSCPEFENEFKDGQSIEYFSDEASLLNKISKILSSSDVDRKSL
                     IDNAYNIILDRHTYNHRAKELLKYIQ"
BASE COUNT          609 a          421 c          368 g          629 t
ORIGIN      
        1 acaatctgga attctcaatg aatgggtatt cgaaatagcc tttgttaatc ctgctccgta
       61 tttgccattt cccaaaacgc caacaagcag ccaaaacatc gtgtttggtc aaagggtgct
      121 ttttgaaaaa gcgaattgta ttagataaac ccataaaata tacttatagt atttttgaca
      181 attgaagcaa aaactgaatc aatatgcgct gattactttc attaatcctt cactgagagg
      241 cgtcaactct ctgttaagta ccattttcat tttttcaata tccggctgcc ttctgctcat
      301 atcaccttct ttgagtggtg gcaggcttac tatttttgat ttagaaccgg taagttgaat
      361 aatggactct gccaactcat tgatagtaac aatcttatca ttccccacat ttaccacatc
      421 atttatataa agcttatcct ccaatatttt taatgtaaaa tcgagattat cttgaatata
      481 acaaaaggtg cgggtctgtg agccatcgcc atatactgtt atatcctggt tgtttcttgc
      541 agcatccagg aatttcgata ccacaaaatc tctgctctgt cttggcccat aggtattaaa
      601 aaaacgaagt atggtataat tcagaccaaa ttcctgatga tatgaccggc aaaaacattc
      661 cccgatattt ttcaatacag cataaggcaa gcgcgagttt aaaggcgtaa catgttcatt
      721 ttgtggcaaa tgtacgggtt cgccatatac ttctgacgat gagctataaa atatgcgctc
      781 aacatccgta ttttttgata attcgaatat gttctgcaaa cctttgatgt catcaaggac
      841 gagtttcggg tgctcgagtg tacgcaatac gccaacaact gcggcataat gaaagacata
      901 gtcaaaatca ttgtacaaca taatggcact aatgtcagcg taatcgttgg catctgcttt
      961 gatgaatttg taattcggat gttgcagaat atgttcctga cgacctgtca acatattgtc
     1021 tatggctgta acatgatatc gtccggttga taacagatgc tctgccaacg aactcggaat
     1081 aaagccggca ccaccggtaa tgagtacttt tttcattttt attgaatata ttttagaagt
     1141 tcttttgcac gatggttata tgtgtgccga tccagtatta tattgtatgc gttgtctatc
     1201 aatgatttgc gatcaacatc agaagatgaa agaatctttg aaattttgtt caaaaggctt
     1261 gcctcatcag aaaaatattc aattgattgc ccgtctttga attcattttc gaattcagga
     1321 catgagctgc tcaactgaag tcctttagaa acaggtattt caaaaaaacg catattagct
     1381 gccggataat tggtatcgtc aataatattg aggttaatgc gagaactatt cagcagttcg
     1441 gcaaaagcat tcccataagc tggcccatca tcaatgatgc ttcgcaatga tttaatcttg
     1501 caacgtttca gccattcagg accttttatc aacatgtcgg cattctgaaa attctctcgt
     1561 atcagtttca aatattgctc tctttcaggt cgccaacctc caatgaagct gatgtcgctg
     1621 ctatatttat ccggcaaatt ttctttgtta tgctgaaaac tgtcggcggc aagaggaagc
     1681 caaaacacat tattaaaacc tgcctgttcg aaaactgcaa cagatgtgct actgtaagta
     1741 cccacaaagt caaacaattt actgttcgca gctactttag gcgttagatt aaagagcgta
     1801 tccggccata acagcccaaa tttagccttg gtaacagacc gcaggaacgc caaagtgcca
     1861 tataatacct tagtattaca aaacaccagt acaagttggg gcgcaaaatc ctttagggac
     1921 agtgccagct cccgatttcc cttcttctcc caagactcaa taccaatatg ggtttctact
     1981 atctggccga acttctttaa aggtatatgc cgattaatat actgtgc
//
LOCUS       DAOQQY010000396         1977 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_34223_length_1977_cov_6.132154, whole genome shotgun sequence.
ACCESSION   DAOQQY010000396 DAOQQY010000000
VERSION     DAOQQY010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34223_length_1977_cov_6.132154"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="PLE23_12805"
     CDS             <1..466
                     /locus_tag="PLE23_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HMX84193.1"
                     /translation="TSFASVFVHPLVDDSIEVEINPADLEWDTFRASGAGGQHVNKTE
                     SAVRVRHIPSGVVCECQQERSQHMNREKALQMLKSRLYELELQKQLEAKDAIEAEKMK
                     NEWGSQIRSYVLDDRRVKDHRTGFQTGNTDAVLDGDIDGFLKAYLMHHEDKP"
     gene            638..1492
                     /locus_tag="PLE23_12810"
     CDS             638..1492
                     /locus_tag="PLE23_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84194.1"
                     /translation="MLALLACIGVLLGRGWQHVFWDPPYRTLLWDENLLKPLIQKLFG
                     ISWEQYVTNNTIDHNFQTSFKIIGGLLSIGAVFAIFINKLKRIAVVYLLFCAAWLLLL
                     SVIYWKEYFWDLPQFIELTSQWMAPVIFVALYKSFNRNINILRILRITLALTFCGHGL
                     YALGFVPLPGSWVDMVINAFSISETGAKHFLKTIGVLDILAAVLVFIPKTERFGYWYM
                     FFWGTIAALGRLVGTFYPEIGILNFLYQQIGEFVYRFPQFLFPLMALFIIKESKRQRS
                     QLNAITDH"
     gene            complement(1498..>1977)
                     /locus_tag="PLE23_12815"
     CDS             complement(1498..>1977)
                     /locus_tag="PLE23_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84195.1"
                     /translation="HYDNNETESSKNVYLSAYTEKIKQMVFRPTQKTFLPFIGDQNEI
                     FSPKHRQKYIFKLRRINIAGKEAYEFDIRRKPNVPDSDILIDKMVTWFDASDLQVIRR
                     TMTVRKKGGFFDLDVNIDVGTTKTSGLYVPAYMKYTGNWHIPFSKKERISFKTTFQY"
BASE COUNT          565 a          378 c          404 g          630 t
ORIGIN      
        1 gacttcattc gcttcggttt ttgtacatcc cttggttgat gacagtattg aagtagaaat
       61 caatccggcg gatctcgaat gggatacctt cagggcgagt ggtgcaggtg gacagcatgt
      121 taacaaaaca gaatcagctg tgcgtgtaag acatattcct tcaggtgtag tatgcgaatg
      181 tcaacaggag agatcacagc acatgaacag ggaaaaggca ttgcaaatgc ttaaatccag
      241 gttatatgaa ctagaacttc aaaagcagct agaagcaaag gatgccattg aagcagaaaa
      301 aatgaagaat gaatggggta gccaaatcag aagttatgta cttgacgacc gaagagtaaa
      361 ggatcaccgt acgggctttc agacaggaaa tacagatgca gtcttggatg gcgatatcga
      421 tggttttctc aaagcttacc tgatgcacca cgaagataaa ccttaaatga aaggcggacg
      481 atttttagat cagccgcctt ttttatttta cggctagtta ttaaacatat tcaaatagtg
      541 agtattctta tttctatctg ttaactttgt attctactaa gcagacaaaa tgatttttgc
      601 ggaagaattc aacaaccgga atcttaaaat aatattcttg ctcgctttac tggcctgcat
      661 cggggtgttg ttaggaagag gctggcaaca cgtcttttgg gatccaccct acagaacgct
      721 cttatgggac gaaaacttat tgaaaccctt gatccagaaa ttattcggta taagctggga
      781 acaatacgtt accaataaca caatagacca caatttccag acgagtttca aaatcatcgg
      841 aggtctctta agtataggtg cagtttttgc catttttatc aataaattga agcggatagc
      901 tgttgtttac ttgttgtttt gtgctgcctg gttgctgtta ttatcggtaa tctactggaa
      961 agaatatttc tgggatcttc cccaattcat tgaactgaca agccaatgga tggcacctgt
     1021 cattttcgtg gctttgtata aatcatttaa ccgtaacatt aacattcttc gaatactacg
     1081 cattacacta gctcttactt tttgtggtca tggtctgtat gcgctgggtt ttgtaccctt
     1141 gccaggcagt tgggtggata tggtcatcaa tgctttctct atttctgaaa ccggagctaa
     1201 acatttcctc aaaactattg gagtacttga catattggct gctgtacttg tgtttatacc
     1261 caaaacagaa cgttttggct actggtatat gttcttttgg ggcactattg cagctttagg
     1321 cagactcgta ggaacatttt atcctgagat aggcatatta aatttcttat atcagcagat
     1381 tggtgagttt gtttatcgct ttccgcaatt tttattccca cttatggccc ttttcatcat
     1441 caaagaatca aaacgacaaa ggtctcaact taatgcaata acagatcatt aaagctatca
     1501 gtactggaat gttgttttga acgaaatccg ctccttctta ctgaaaggaa tatgccagtt
     1561 tccagtatat ttcatatatg ccggaacgta aagtccactt gtttttgtgg tgccgacatc
     1621 tatattgaca tccaaatcaa aaaaaccgcc tttcttcctg acagtcatcg tacgtctgat
     1681 aacctggagg tcacttgcat caaaccatgt aaccatcttg tcaatcaaaa tatcggagtc
     1741 gggcacattt ggttttcgcc taatgtcaaa ttcgtatgct tctttgcctg caatgtttat
     1801 tcttcgaagc ttgaatatat atttttgcct gtgctttgga gagaaaatct cattctggtc
     1861 gccaataaaa ggaaggaatg tcttctgtgt cggtctgaac accatttgtt taatcttctc
     1921 ggtataggct gataaataca catttttact tgactcggtt tcgttgttat catagtg
//
LOCUS       DAOQQY010000397         1971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_34421_length_1971_cov_5.403445, whole genome shotgun sequence.
ACCESSION   DAOQQY010000397 DAOQQY010000000
VERSION     DAOQQY010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34421_length_1971_cov_5.403445"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PLE23_12820"
     CDS             complement(<1..360)
                     /locus_tag="PLE23_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HMX84196.1"
                     /translation="MKPISYLLILGLCFQFLACQQSKENEKKATEKYTVTNPVIKDTS
                     ILKEYTAQIQSEQNVEIRAQVKGYLEGINVDEGQHVKAGQVLFTIMPKEYEAKYQKAK
                     AEYEQAELEWKNTKSLAD"
     gene            complement(552..914)
                     /locus_tag="PLE23_12825"
     CDS             complement(552..914)
                     /locus_tag="PLE23_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84197.1"
                     /translation="MKKLITGIILIVSSFVNTSHVTACSLSFSEYFKNLQYSQQHSRL
                     LSHCVNDSNITDLNYYPEDDTNETNDKTSSCDSVIFPDNRHKYVYPLNGYSYNFVFSS
                     LRLHTSLPIYLLNEVFII"
     gene            complement(1080..1757)
                     /locus_tag="PLE23_12830"
     CDS             complement(1080..1757)
                     /locus_tag="PLE23_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84198.1"
                     /translation="MKRRIFFLLFMIPWALSAQNPKTLLNNAFKKFAKVQNYIADVHM
                     DFDLPSVSMDAVTGQVFYKSPNKFRIKSKGIAFVPKQNPYFSLNELRDTNSYVAVLQG
                     AEKIRNINCRIINVIPNNPTDMILGKFWIDAANNVILKSQITTASNGTIQLDNYFGTQ
                     KNFALPDKMVITVEMTKFKMPKALSAELNSKSSKTSSKKEKGTGKISMDFTNYKINTR
                     LNEDVFK"
     gene            complement(1758..>1971)
                     /locus_tag="PLE23_12835"
     CDS             complement(1758..>1971)
                     /locus_tag="PLE23_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84199.1"
                     /translation="LFTIIGTLDIPLFDHIKFFLFTTHMIVWRNLFDNPLDFNLIFTS
                     VGILIGHIFVFNALAFYYFKRKDIQS"
BASE COUNT          547 a          311 c          417 g          696 t
ORIGIN      
        1 gtcggcgagc gatttggtgt ttttccattc gagttcggcc tgttcgtatt ctgcctttgc
       61 tttttggtat tttgcttcat attccttagg cataatggtg aacagcacct ggcctgcctt
      121 tacgtgttgt ccttcatcta cgttgatgcc ttcgagatac ccttttactt gtgctctgat
      181 ttctacattc tgctcagatt gtatctgagc tgtatattct ttaagtatag atgtatcttt
      241 tataaccggg ttggttactg tatatttttc tgttgctttt ttttcatttt ctttactttg
      301 ttggcaagct agaaactgaa aacacaggcc gagtattaat aaatatgata taggtttcat
      361 aaaagttatt ctttgattgt tttaaaaatg aatattccgc aaagaattca atagttcaat
      421 tcagctaaat tgacagtatt gagcagatat attttgcatt aagtaataga ggcagcctac
      481 ggttttactg agcggttttt attccgggct gtaatgtcaa tgagcattga cgctatgtgt
      541 atggaaagat atcaaataat aaacacctca ttgaggaggt aaataggcaa gcttgtgtgt
      601 aatcttaaag atgagaatac aaaattatag ctgtacccat tcaaaggata gacatattta
      661 tgcctattgt ccggaaaaat aacagagtca cagcttgaag tcttgtcgtt ggtttcgttt
      721 gtgtcatctt cggggtaata attcaaatca gtaatattgg aatcattcac acaatgtgat
      781 agcagccttg aatgttgctg gctatactga agatttttaa aatattcgga aaaagaaaga
      841 ctacaagcag tcacatggct cgtattgaca aacgagctca caataagaat aatgcctgta
      901 attaactttt tcatatcggt agcaaattta ctggtattag tgttttgagc aaatttcgca
      961 aggaggatta agcatttttt aacatcatgc taaaatctta acatttatca gacgcaaata
     1021 tttcaatgtc tattacaatg ttgtacctca ttactaaagc cattttttat taattttttt
     1081 catttaaata catcttcatt aagccttgta ttaatcttgt agtttgtaaa atccatgctt
     1141 atcttgcctg ttcctttctc ttttttgctt gaggtctttg atgattttga attaagttct
     1201 gcagagagtg ctttaggcat cttgaatttg gtcatttcga ccgttataac cattttatca
     1261 ggcagagcga agtttttttg tgtgccgaaa tagttgtcga gctggatagt gccgttggat
     1321 gctgtggtta tctgcgactt taagatgaca ttgttggcgg catcgatcca gaatttgccg
     1381 agaatcatgt cggtcgggtt gttaggaatg acgttgatga tgcggcaatt gatgtttcgg
     1441 attttttcgg cgccttgtaa tacggctacg taactgttgg tgtcgcggag ctcgttgagt
     1501 gaaaaatatg gattctgctt gggtacgaaa gcaatacctt ttgatttgat gcgaaatttg
     1561 ttggggcttt tatagaaaac ctgaccggta acagcatcca tggatacgct gggcaggtcg
     1621 aagtccatgt gaacgtccgc aatatagttc tgcactttag cgaatttttt gaaagcattg
     1681 ttgagcaatg tttttgggtt ttgggcggat agtgcccatg gtatcatgaa taataggaag
     1741 aagattctgc gtttcattta gctttgaatg tcttttcttt tgaaataata aaatgccagt
     1801 gcattaaaaa cgaatatatg accaataagt atcccgactg atgtgaatat taagttaaag
     1861 tcaagcggat tgtcaaacaa attgcgccag acaatcatgt gtgtggtgaa aaggaagaat
     1921 ttgatgtggt caaataatgg tatgtcgagt gtgccgataa tcgtaaacag g
//
LOCUS       DAOQQY010000398         1957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_34840_length_1957_cov_4.380652, whole genome shotgun sequence.
ACCESSION   DAOQQY010000398 DAOQQY010000000
VERSION     DAOQQY010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34840_length_1957_cov_4.380652"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLE23_12840"
     CDS             <1..528
                     /locus_tag="PLE23_12840"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMX84200.1"
                     /translation="KVNVRIIAATNRDLKKEIESGNFREDLFYRIAVFEINLLPLRER
                     LADIEDLSYFFLKKFEESANRKISSISNEYLELLMQHQWKGNIRELRNVIERSVILCE
                     GTILTPESLPYEIQQLSKPGTKEKMLSAFELASAEKVHIQKVLNYTNGNKTKTAELLG
                     IALTTLYRKMSEYNL"
     gene            548..1186
                     /locus_tag="PLE23_12845"
     CDS             548..1186
                     /locus_tag="PLE23_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMX84201.1"
                     /translation="MAFKTIKEIFAHNQEWIAEQLKDDPEYFSKLSKQQQPKYLFIGC
                     SDSRVSTEALMYSKPGDVFIHRNIANVIPSNDLNVLSVINYAVNHIKVKYIVVCGHYG
                     CGGIKTAITATDAGILNPWMGYIRDVYRLHKKELNAIKSIAKRSDRLVELNVQEQCIN
                     LVKTRDIQQALLENRIEIHGWVFDMRTGKLIDLGFNTQKVAKEVLDIYNIVK"
     gene            1434..1721
                     /locus_tag="PLE23_12850"
     CDS             1434..1721
                     /locus_tag="PLE23_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84202.1"
                     /translation="MMNSNEKLNIYKQAQRFADVTKKLIITGNVKRAKRCLNVASNLM
                     EKGNKEIQNAISNVYVYSLSSFMELHHCAIKNFFPKNLQNEYHNQINASGL"
BASE COUNT          717 a          348 c          333 g          559 t
ORIGIN      
        1 aaagtaaatg tacgaatcat tgcagctacc aatagagatc tgaaaaaaga aatagagtct
       61 ggcaattttc gtgaagatct gttctaccgc attgccgtat tcgaaataaa ccttctaccg
      121 cttcgtgaaa gactagccga tatagaagac ctgagttatt tcttcctgaa gaaatttgaa
      181 gaatctgcaa acagaaaaat ttcctccatt tcgaacgaat atctagaatt gcttatgcag
      241 catcaatgga agggaaatat acgggaatta cgcaacgtca ttgagcgaag cgtcatactc
      301 tgtgaaggca ctatcctcac ccccgaatca ttgccttatg aaatccagca attgagtaag
      361 cccggcacaa aagaaaaaat gctgtcagcc tttgagctgg caagtgcaga aaaagtgcac
      421 atacaaaaag tgctgaacta cacaaacggc aataagacaa aaacagctga actgttaggt
      481 attgcactta ccaccttata ccgcaagatg agtgaatata atttataata acaaaaacag
      541 gtttatcatg gcattcaaaa ccatcaagga aatattcgca cacaatcaag aatggattgc
      601 agaacaattg aaggatgatc ctgaatattt ttcaaaatta tcaaaacaac aacaacccaa
      661 atatctattt ataggttgct cggacagccg tgtctctaca gaggctttga tgtattcaaa
      721 accaggtgat gtatttattc acagaaacat tgccaatgtt atacccagta atgatttgaa
      781 tgtactttca gtcattaatt atgctgtcaa tcatattaaa gttaaatata tagtggtgtg
      841 cggccattat ggttgtggtg gtataaaaac tgcaattact gctacagatg cgggtattct
      901 gaatccttgg atgggctaca tccgcgatgt gtaccgctta cacaaaaaag aactgaacgc
      961 tataaaatct atagccaaaa gatccgacag actggtcgaa ttgaatgtac aggaacaatg
     1021 tatcaatctg gtcaaaacac gagacatcca acaagcttta ttagaaaaca gaatagagat
     1081 acacggttgg gtttttgata tgcgcacagg caaactgata gatttaggct tcaatacaca
     1141 aaaggtcgca aaagaggttt tggatattta taacatagtg aaataatata aaccattaaa
     1201 gtattaagca tagttaaggt acattctcaa tttcctaaaa ttcttaatgg ttcaaaaaca
     1261 tcttccaacc ctttcaaaac gctaatattt accctttcaa aatgaaaggg ttttttgttt
     1321 tgaaaatctg taaacattta catatctatt tgatttactg cgtgttacac cttataaaaa
     1381 tactttggaa catcatttga aatataattg gcataatcaa ttataaattt atcatgatga
     1441 actcaaatga aaaactcaac atctacaagc aagcacaacg atttgctgat gttactaaaa
     1501 aattaatcat caccggcaac gtcaaacggg ctaaaagatg cctgaatgtc gcttcgaatc
     1561 tgatggaaaa aggcaacaaa gaaatacaaa atgcaatatc gaatgtgtat gtgtattctt
     1621 tatcgtcctt catggagctc catcactgtg ccataaaaaa cttcttccct aagaatttac
     1681 aaaacgaata tcataaccaa ataaacgcaa gcggactatg acagcaattc tccttatcct
     1741 tttaggcgta gccctatttt cattctttta ttatttaatc aacttatttg aaaagatatg
     1801 acagctttat ttatcatcgg aatattggtt tttgggtaca tcagttatgt attagtaaaa
     1861 ccggagaaat tttaattagt gaatagtgaa tggtgaagaa ccatagactt tctcaataaa
     1921 gtgtagacga agataagtta aaaacaaata acttatt
//
LOCUS       DAOQQY010000399         1941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_35346_length_1941_cov_6.783669, whole genome shotgun sequence.
ACCESSION   DAOQQY010000399 DAOQQY010000000
VERSION     DAOQQY010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35346_length_1941_cov_6.783669"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..692)
                     /locus_tag="PLE23_12855"
     CDS             complement(24..692)
                     /locus_tag="PLE23_12855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HMX84203.1"
                     /translation="MLNQTHLNFLQQLKQNNNRPWFNEHKAAFDKHYSEVKAFLMNIY
                     NLLQDTDMLEPIHIHRIYRNLQFSKDKTPYKTHFSLHIGRTKPLLRGGYFLNIEPGNS
                     FAAGGFWKPEAADLLRFRKEVNIDATPLRAVLSAKTAVRYFGALVGEELRTVPAGFNK
                     DSDAIDLLRKKQWLLIRRFADPEVVQKDFGNEVVKTFHALRPFFDYMSEVLTTDENGE
                     SLFG"
     gene            complement(761..>1941)
                     /locus_tag="PLE23_12860"
     CDS             complement(761..>1941)
                     /locus_tag="PLE23_12860"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMX84204.1"
                     /translation="CTEKIDSLYDFLQQKDSKAFMVLKDGKIVLEKYFGTFTQDSIWY
                     WASAGKTLTGFLTGIAQEQGYLSINDKSSKYLGSGWTSLDTAQENKISIRHQLTMTTG
                     LDDGDDPDCTLPSCLTFKAEPGTRWAYHNAPYTLMDSVIESATGKNYNQYFTQVVKSK
                     TGMTGLWVKTGYNNIFYSNTRSMARFGILMLNKGTWNKTPVLADTVYFNAMIHSSQNM
                     NKSYGYLTWLNGKQNYMLPGSQIVFNGSLMKDAPEDMFAAMGKNGQIINISPSLGLVV
                     IRTGNPPNGSSGFVPNLFNNDIWKYLNKVICKTTSTNSISTKQNNLIVTNPVQNILYL
                     NRDAVTGRFKLEIIDINGNILLTDENNTEPDISNLKPGLYYVRIISSDKFKLQPFVKI
                     "
BASE COUNT          505 a          365 c          419 g          652 t
ORIGIN      
        1 atgtttaaaa aaatttcatt aaattaacca aacaagcttt caccattttc atcagttgta
       61 agcacttccg acatatagtc gaaaaatggt cggagtgcat gaaaagtttt tacaacttca
      121 ttcccgaagt ctttctgcac aacttccgga tctgcaaaac gccgtatcaa cagccattgt
      181 ttcttgcgca aaaggtcgat tgcatccgag tctttgttaa atcctgccgg tacggttcgc
      241 agctcttcac ctactaatgc tccgaaatat cggactgcag ttttggcaga cagtactgct
      301 cttagtggag tagcgtcaat attgacttct tttctaaacc tcagtaaatc agcagcttct
      361 ggcttccaga atccaccggc tgcaaagctg ttgccgggtt cgatgttgag gaaataccca
      421 cctcgcagta atggtttggt ccggccgatg tgtaggctga aatgcgtttt gtaaggtgtc
      481 ttgtcttttg aaaactgtag attgcggtat atacggtgaa tgtgaatcgg ctcgagcata
      541 tctgtatcct gcaagagatt gtagatgttc attaaaaatg cttttacttc gctataatgt
      601 ttgtcgaatg ctgctttatg ttcattaaac cacggccggt tgttgttttg tttcagctgt
      661 tggagaaaat tgaggtgcgt ttggttaagc atgaattaat tattaatcgt aaatatacga
      721 tattattatt tatttttaaa taatgattga ataatactgc ttatattttt acaaatggtt
      781 gaagtttaaa tttgtcagat gaaattattc tcacataata caagccgggt ttcaaattac
      841 ttatatcggg ttctgtgttg ttttcgtctg tgagcaggat atttccgttt atatctatta
      901 tttcgagctt gaatcttccg gtaacagcat ctctgttaag gtagagaata ttttgtacag
      961 gattggttac gatcaaatta ttctgcttag tggatatact attggtactt gtggttttac
     1021 atattacttt gttcagatat ttccatatat cgttgttgaa caggtttggt acaaacccac
     1081 tcgagccatt gggcggattt ccggtgcgta ttaccaccag accaagggaa ggagaaatat
     1141 tgataatttg accgtttttg cccattgccg caaacatatc ttcgggtgca tctttcataa
     1201 gagagccatt gaatacaatc tgcgaaccgg gcagcatata gttctgtttg ccgtttagcc
     1261 aggtaaggta tccataagat ttgttcatgt tttgagaaga atgaatcatg gcattgaaat
     1321 agacagtgtc tgccaacaca ggtgttttat tccaggtgcc tttgttgagc ataagaatac
     1381 caaaccgtgc cattgatctt gtattgctgt agaaaatatt attgtagccg gtttttaccc
     1441 aaagcccagt catgccggtt ttacttttta ccacctgagt gaaatactga ttgtaatttt
     1501 ttcctgtagc tgattctatt acgctgtcca tcagcgtgta aggagcatta tggtaagccc
     1561 atctggtacc gggttctgcc ttgaatgtaa ggcaagaagg aagtgtacag tcggggtcgt
     1621 cgccatcatc aagaccggtg gtcatggtga gttgatgcct gatgcttatc ttgttttcct
     1681 gagctgtatc aaggctggtc cagccgcttc cgaggtattt ggatgattta tcattaatgg
     1741 acaggtaacc ttgttcttga gcaataccgg taaggaatcc tgtgagtgtt ttgcctgctg
     1801 atgcccagta ccagatgctg tcttgcgtga atgtgccgaa atatttttca agaacaatct
     1861 tgccgtcttt taataccata aaggcttttg aatccttttg ctggaggaag tcatagagcg
     1921 aatcgatctt ctcagtacac c
//
LOCUS       DAOQQY010000400         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_35898_length_1924_cov_4.446228, whole genome shotgun sequence.
ACCESSION   DAOQQY010000400 DAOQQY010000000
VERSION     DAOQQY010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35898_length_1924_cov_4.446228"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            184..1146
                     /locus_tag="PLE23_12865"
     CDS             184..1146
                     /locus_tag="PLE23_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002657165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMX84205.1"
                     /translation="MSVLVFAETHDNTFKKPALEAVFYGKKTASMLGTQCIALVLGDA
                     ANAAQLGKYGADKVLHLTDAALNSFDSQVYASVITQVAQTTGSDVVVLVHSAEGKAIT
                     GRVAVKLNAASVSGANSLPELSNGFTVAKSVYSGKAKVHYTLNSDNKVISIMGNSLPI
                     EANGNDAPVESVSVNIPAARVKTTKVDRITGEVPLPEAEVVVSGGRGLKGPENWGILE
                     DLGKVLGATMACSRPVADAHWRPHHEHVGQTGIAIRPNLYIAVGISGAIQHLAGVNNS
                     KVIVVINKDPEAPFFKAADYGVCADLFDVVPKLTEAIKKFKAQH"
     gene            1186..1773
                     /locus_tag="PLE23_12870"
     CDS             1186..1773
                     /locus_tag="PLE23_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HMX84206.1"
                     /translation="MNKVELQIVALSHSMTQSQNYAVVLGEKEGARRLPIVIGGFEAQ
                     AIAVAMEKLSPNRPLTHDLLKNAFDAFGIYLREIVISNLVDGIFYSKLVCDRDGEVIE
                     IDSRTSDAIALAVRYECPIYTYEFILEAAGVILEEPDAADRPKKKEPKKKKEDLSTLS
                     LDDLQSMLDEALGNEDYEKAAKIRDELNHRKQLGQ"
BASE COUNT          597 a          364 c          480 g          483 t
ORIGIN      
        1 ccactcgaag taatagaacc ggcagcattc actgaaaatg ttgtgaccag gcaatttgac
       61 ctgccagcac ctaagtcggc tgttaagatg atagatgcag ataacatgga tgagttggta
      121 agattgctgc atgaggaggc gaaagttata taaataaaaa attcaaataa aaaaattaag
      181 gaaatgtctg ttttagtatt tgcagaaact cacgataata catttaaaaa acctgcactc
      241 gaggcagtgt tttatggaaa aaaaacagct tcaatgcttg gtacacagtg catagcactt
      301 gtacttggcg atgctgccaa tgcggctcag ctaggaaaat acggagccga caaggtatta
      361 catttgacgg atgctgcact taatagtttt gactcgcagg tatatgcttc tgtaataact
      421 caggtggcac agactacggg cagcgatgtt gtagtattgg tacacagtgc tgaaggcaaa
      481 gcaatcacag gaagggtagc ggtcaagctg aatgctgcat cggtgtcagg cgccaacagt
      541 ctgcccgagc tgagcaatgg ttttacagtg gccaagtcag tatattcggg taaggcaaag
      601 gtgcattata cactgaactc agacaacaag gtcatcagca tcatgggcaa tagtctgcct
      661 atagaagcaa acggaaatga tgctcccgta gagtctgtat cggtcaatat accggcggct
      721 agagtaaaga cgactaaggt ggacaggata accggtgaag tgcctcttcc tgaagctgaa
      781 gtagtagtat cgggcggacg cggactcaag ggtcctgaaa actggggaat tctcgaagac
      841 ctcgggaaag tacttggtgc cacgatggca tgctcaagac cggtagcaga tgcacactgg
      901 agacctcacc acgagcacgt aggacagaca ggtattgcca tcagaccaaa tctgtatata
      961 gcagtaggta tttcgggtgc catccagcat ctggcgggcg tcaacaactc gaaggtaatc
     1021 gtagtgatca acaaagatcc ggaagcacct ttcttcaagg cagcagatta tggtgtgtgt
     1081 gccgatttgt ttgatgtggt accaaagctg acggaagcaa taaagaaatt caaggctcag
     1141 cattaaaaat cagggcgttg gacatagaat gagagaacaa agacaatgaa taaagttgaa
     1201 ttacagatag ttgcattgtc tcatagcatg acacagtctc agaactatgc tgttgtgtta
     1261 ggagagaagg aaggtgctcg aagactgccg attgtcattg gtgggtttga ggctcaggca
     1321 atagcagtag ctatggaaaa actgagtcct aacaggccat tgacgcacga cctactaaag
     1381 aatgcttttg atgcttttgg tatttacctg cgtgaaatag tgataagcaa tctcgtggac
     1441 ggcattttct attcgaaact cgtttgcgac cgcgacggtg aagtcataga aatagattcc
     1501 cgtacatcgg atgccatagc gctggcggta aggtacgagt gccctatcta tacatacgaa
     1561 tttatcctcg aagctgccgg tgttatactc gaagaacccg atgctgctga cagacccaag
     1621 aagaaagaac ccaaaaagaa aaaagaagat ttgtcaaccc tcagcctgga tgatttgcaa
     1681 agtatgctcg atgaagcatt ggggaatgaa gactacgaga aggcggccaa aatacgggat
     1741 gaattaaacc acaggaagca gttgggacag taaatatcta tccaatccaa attattgttt
     1801 tacttaagta cgttgtatgc attcctgcca atattggtag gaatttagca tttggtattt
     1861 tgttacattt ttgttaaaag tgttttattt atttatttat tgtaccttgt atttgatatt
     1921 catt
//
LOCUS       DAOQQY010000401         1908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_36459_length_1908_cov_4.022126, whole genome shotgun sequence.
ACCESSION   DAOQQY010000401 DAOQQY010000000
VERSION     DAOQQY010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36459_length_1908_cov_4.022126"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="PLE23_12875"
     CDS             <1..553
                     /locus_tag="PLE23_12875"
                     /inference="COORDINATES: protein motif:HMM:NF039190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5672 family protein"
                     /protein_id="HMX84207.1"
                     /translation="EDTYFGSIQGYNRLLLSTKFYKAFEAFDYILITQLDVWVFSNQL
                     QHWCDKGYDYIGAPWLERPPLQKKINLLPMGKWMYRKVGNGGFSLRKVGTHTYISSKM
                     KSISWLFNKNEDFFWSIVAPKIFRWFKIPALEEAVFFAFEIAPAQAYELTGHQLPFGV
                     HAWQKHQPEFWKNLIPLSENQQK"
     gene            550..1653
                     /gene="wecB"
                     /locus_tag="PLE23_12880"
     CDS             550..1653
                     /gene="wecB"
                     /locus_tag="PLE23_12880"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013765802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMX84208.1"
                     /translation="MKIIQVVGARPNFMKVAPLQRAFQKFPEVQTLIVHTGQHYDSAM
                     SDIFFRQLELPQPDYFLGVGGGSHTEQTAKIMLAFEKILTDEKPDIVLVVGDVNSTIA
                     CALVAVKMNIAVAHVEAGLRSNDRTMPEEINRILTDSISDWLFVTEPDGLRNLRNEGV
                     SEEKIHYVGNTMIDSLAYYLPKLDEIDLPEDLHEKGINPNEYIIVTMHRPSNVDTEEG
                     LKQTLSVIKLAAGYKKVVLPLHPRTKNNLQKFGLENEIFNHHDIIITEPLGYLPFIKL
                     LKDSWLVVTDSGGIQEETTYLKIPCLTFRKSTERPITVTAGTNTMISELNVALVKEKI
                     EELAAGNYKKGQIPERWDGHTAERIAKILVKPG"
BASE COUNT          626 a          389 c          389 g          504 t
ORIGIN      
        1 tgaagacact tactttgggt caatacaagg ctacaatagg cttttacttt caaccaagtt
       61 ttacaaagct tttgaagcat ttgactacat tctcatcacc caactcgacg tgtgggtatt
      121 cagcaaccag ctccagcatt ggtgcgataa aggctatgat tatataggag cgccctggct
      181 tgaaagacca ccattgcaaa agaaaataaa tctgttaccc atgggcaaat ggatgtatcg
      241 caaagtagga aacggtgggt tttcactcag gaaagttggc acgcacacat atatttcatc
      301 caaaatgaaa agcatatcat ggttattcaa taaaaatgaa gacttctttt ggagcatcgt
      361 ggcaccaaaa atattcagat ggttcaagat acctgcactt gaagaggctg tattctttgc
      421 attcgaaatt gctccggcac aagcttatga actcaccggc catcagctgc cattcggcgt
      481 acatgcctgg caaaaacacc agcccgagtt ctggaaaaat ttgatacctt tgtctgaaaa
      541 tcaacagaag tgaaaataat acaggtggta ggtgcaagac cgaatttcat gaaagtggca
      601 ccacttcaga gggctttcca aaaatttccg gaagtgcaga cacttatcgt acacacaggt
      661 caacattacg atagtgcgat gagtgatatt ttcttcagac aattagaact ccctcagccc
      721 gactatttct tgggagtagg tggtggatct cataccgaac aaacggcaaa gattatgctt
      781 gcctttgaaa aaatcctgac tgacgagaaa cctgatattg tgcttgtagt tggtgatgtt
      841 aattctacta ttgcgtgtgc acttgttgct gtaaaaatga atatcgcggt ggctcacgta
      901 gaagccggcc tacgatcgaa tgacagaaca atgcccgaag aaataaacag gattctcaca
      961 gacagtatat ccgactggct tttcgtaacc gaacccgatg gcctaaggaa tctaaggaac
     1021 gaaggtgtaa gtgaagaaaa aatacattat gtaggaaata ctatgattga ttcattggca
     1081 tattatctac caaaattgga tgaaatagac ctaccggaag atttacacga aaaaggaata
     1141 aacccaaatg aatatatcat cgtcactatg caccgcccat cgaatgttga tactgaagaa
     1201 ggcctgaagc aaactttgtc tgtcatcaag cttgctgccg gatacaagaa ggttgtattg
     1261 ccgttgcatc cgcgcacaaa gaataatctt caaaaatttg ggttggaaaa tgagattttt
     1321 aaccatcatg acatcatcat aaccgaaccg cttggttatc tgccatttat caaattgctc
     1381 aaagatagtt ggctcgtagt gacagactcc ggtggcatcc aggaagaaac gacttatctg
     1441 aaaattcctt gcctgacatt cagaaaatca acagagcgcc ctataactgt aaccgcgggt
     1501 accaacacca tgataagtga gttgaatgta gcgttggtga aggaaaaaat cgaggagtta
     1561 gcggccggaa actacaagaa aggacaaata cccgaacgct gggacggaca taccgcagaa
     1621 cggatagcaa aaatacttgt taaacccgga taggcattag gctctttgct gacttcggca
     1681 ctttttttaa aaacgagcgt attattgttt ttaaatattt attaatcaat tgtttaggag
     1741 aattagtttt aatattttgt gtgtcccagg acggaaaaca gcaataataa tgtatcacaa
     1801 catcttacga cttactactt accacttact acttaccact taccactcac catctgcgta
     1861 ttctatggta aaaaccaagc actcacatta tttttttcaa gaaagata
//
LOCUS       DAOQQY010000402         1907 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_36492_length_1907_cov_4.265659, whole genome shotgun sequence.
ACCESSION   DAOQQY010000402 DAOQQY010000000
VERSION     DAOQQY010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36492_length_1907_cov_4.265659"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLE23_12885"
     CDS             <1..524
                     /locus_tag="PLE23_12885"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMX84209.1"
                     /translation="YIGPKQAEAVKQMARAEQMFAKDSFDLALSNPGGGFPGFKDIAA
                     KYSGTNAGNVANYYAGVCLLKTGKAAEAVPYLEKFSPPGNMLPQMKASLLGDAYADQN
                     NLEKAYSQYEKAAAASNNEAVTPFVLMKMGMLAEKMGKNKEAAAAYQRIKDEYATTGI
                     GRNVDKYLYRVTE"
     gene            611..1129
                     /gene="ribH"
                     /locus_tag="PLE23_12890"
     CDS             611..1129
                     /gene="ribH"
                     /locus_tag="PLE23_12890"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMX84210.1"
                     /translation="MATKDYKENDFKPADLNYPENIKIGIVVSEWHSSITENLLNGSK
                     EVLSKFNISEDNITIIKVPGSFELTMGARILLSSMKLDAVICLGCVIKGETNHDDYIN
                     QAVATGITNLGLSSGKPVIFGLLTVLNQQQAEDRAGGKYGNKGSECALTALQMIGIRQ
                     QFSESKSKIGFS"
     gene            1126..>1907
                     /locus_tag="PLE23_12895"
     CDS             1126..>1907
                     /locus_tag="PLE23_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMX84211.1"
                     /translation="MKLSFIHAGNFKLDGGAMFGVIPKRMWEKLNPADQNNLCSWSMR
                     CLLIEEGKCKILVDTGMGDKQDDKFKSIFEPFGPQTLLGSLKEQGMNPEDITDVFLTH
                     LHFDHCGGAVSRKENGELVPTFPNATYWSSRKHWDWAMNPNAREAASFLKENFVPLLE
                     AGIVKFVEESGQEVFPGVKAEFFFGHTEEMMGLHINNDKGRFFYCADLIPSSFHIGLP
                     YIMAYDLRPLETLTEKTRLLTDAVEQGIYLLFEHDPATGCATV"
BASE COUNT          602 a          410 c          421 g          474 t
ORIGIN      
        1 tgtacatcgg cccaaaacag gctgaagcag taaagcaaat ggcgcgagca gaacaaatgt
       61 ttgctaaaga ctcattcgac ctcgctctta gtaatccggg tggtgggttt cctggtttta
      121 aggacattgc agcgaaatac agcggcacca atgccggcaa tgttgccaac tactacgcag
      181 gtgtttgctt gttaaaaacc ggaaaagcag ccgaagctgt tccatatcta gaaaaattca
      241 gccctccggg aaatatgctt cctcaaatga aagcatctct gctcggtgac gcctacgctg
      301 atcaaaacaa tctcgaaaaa gcatatagcc aatatgaaaa ggcagcggca gcatctaaca
      361 atgaagcagt aacacctttc gtccttatga aaatgggaat gctcgctgag aaaatgggca
      421 aaaacaaaga agccgcagca gcataccaac gaatcaaaga tgaatatgct acaactggta
      481 tcggtaggaa tgttgataaa tatttgtaca gagtcactga ataaacattt ttatttatca
      541 aaattttaga aaaggtaagt ggtattccgc ttaccttttt taattttaca ttacgactaa
      601 atattgaact atggccacta aggattataa agaaaatgat tttaaacccg ctgacctgaa
      661 ttatcctgaa aatatcaaaa taggtattgt cgtgagcgaa tggcattctt ccattacaga
      721 aaatctgctt aatggcagta aggaagtctt aagcaaattt aacatcagtg aagacaacat
      781 caccatcata aaagtaccag gatcgttcga attaaccatg ggtgctcgta tcttgttgag
      841 tagtatgaaa ctcgatgctg tcatctgcct tggttgtgtc atcaaaggtg aaacaaacca
      901 cgatgactat atcaatcagg cagtagctac cggcatcacc aacctggggc tttcttcggg
      961 taaacctgta atattcgggc tgctcaccgt actcaaccag caacaggcag aagaccgtgc
     1021 aggtggaaaa tacggcaaca aaggtagtga atgtgcactt actgctctac aaatgatcgg
     1081 tatccgccaa caattctctg aatcaaaaag caaaattgga ttttcatgaa actaagcttt
     1141 atacatgccg gtaactttaa gctcgatgga ggtgccatgt ttggtgtcat cccgaaaagg
     1201 atgtgggaaa aactcaatcc ggctgaccag aataatcttt gctcctggtc tatgcgttgc
     1261 cttttgatag aagaaggaaa gtgtaaaata ctcgtagata ccggtatggg cgacaagcag
     1321 gacgacaagt tcaaatcgat attcgaacct ttcgggccac agacacttct tggctcattg
     1381 aaggaacaag gcatgaatcc tgaagacatt acagatgtgt ttttgacaca cttgcacttt
     1441 gaccattgtg gcggagccgt cagcagaaaa gaaaacggcg aacttgtgcc aaccttcccg
     1501 aatgccacct actggagcag tcgaaaacac tgggactggg caatgaaccc gaatgcacgt
     1561 gaggcagcct cgttcctgaa ggagaatttt gtaccattac tcgaagcagg catagtaaaa
     1621 tttgtcgaag aatccggtca ggaagtcttc ccgggagtaa aggctgaatt ctttttcggc
     1681 catacggaag aaatgatggg acttcacatt aataatgaca aagggagatt cttttattgt
     1741 gccgacctta ttccttcatc attccatatc gggctgccct acatcatggc ttatgatctg
     1801 aggcctcttg aaaccctgac agaaaaaacc cgcttactga cggatgccgt agaacaagga
     1861 atctatctgc tgttcgagca cgacccggca accggatgtg ctaccgt
//
LOCUS       DAOQQY010000403         1892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_37016_length_1892_cov_6.353838, whole genome shotgun sequence.
ACCESSION   DAOQQY010000403 DAOQQY010000000
VERSION     DAOQQY010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1892
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37016_length_1892_cov_6.353838"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLE23_12900"
     CDS             complement(<1..532)
                     /locus_tag="PLE23_12900"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMX84212.1"
                     /translation="MAVKRRNSQLAPETVGVVGSGSFGKAIANLLSFNCNVMLFTRRA
                     ELVEQVNVHHEFAGIKISPRIVATSNMQEVAATCKVIFPIVPSNKFREMMRSLGPLLT
                     PAHILIHGTKGFDLVGVDYNDLDNNNITRENIRTMSDVILEESSVLRVGCLSGPNLSA
                     EILEGQPTASLIASHFD"
     gene            complement(706..927)
                     /locus_tag="PLE23_12905"
     CDS             complement(706..927)
                     /locus_tag="PLE23_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HMX84213.1"
                     /translation="MYWTLELASHLEDAPWPATRDELIDFAIRSGTPLEVIENLQQME
                     DDGDIYESIEDIWPDYPRRDDFFFNEDEY"
     gene            complement(1026..>1892)
                     /gene="queA"
                     /locus_tag="PLE23_12910"
     CDS             complement(1026..>1892)
                     /gene="queA"
                     /locus_tag="PLE23_12910"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002660590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HMX84214.1"
                     /translation="PARLYGKKEKTGADIEVFLLRELNHEQRLWDVLVDPARKIRVGN
                     KLYFTDDKNNDILVAEVVDNTTSRGRTIRFLYDGTDDQFNADLDYLGNTPLPKYINRD
                     ADTFDKERYQTIYAKELGAVAAPTAGLHFSQELMKRLELKGVNFAELTLHVGLGTFRT
                     IDVEDLSKHKMDAEYFRIPEKAAKYVNDAKEAGRRVCAVGTTSMRAIESSVSAEGFLK
                     AAEGWTNKFIYPPYDFSIANSMITNFHLPKSSLIIMVAAFAGYELTMEAYHEAIKEKY
                     RFYSYGDAMMII"
BASE COUNT          525 a          411 c          366 g          590 t
ORIGIN      
        1 catcaaaatg gcttgctatc aatgaggcag ttggctggcc ttcgagtatc tctgcgctca
       61 aatttgggcc tgacaaacaa cctaccctga gtacagagct ctcttcaaga ataacatcgc
      121 tcattgtgcg gatattctct ctagtaatgt tgttgttgtc aagatcatta tagtcaacac
      181 ctaccaggtc aaatccctta gtgccatgaa tgagtatatg tgccggtgtg agcagaggtc
      241 cgagcgaccg catcatttcc cggaatttat tggaaggcac gatcggaaaa atcactttac
      301 aagtagcagc cacctcctgc atattgctgg tagctactat acgcggtgaa attttgatac
      361 ctgcaaattc gtggtgcaca ttcacttgtt ctaccaactc tgctcttcgg gtaaacaaca
      421 tgacattaca attaaaggaa agcagattgg cgattgcttt accaaagcta cctgaaccta
      481 ccacacctac cgtttccggt gccaattggc tatttctcct ttttactgcc atgataaaaa
      541 tctacgattt gtaaagataa aaacttatac acattaagcc gtgattgata aacaatgttt
      601 ttgacaaagt tacacctccc aacaggctac ggctaaaaac atgacgttac tctaacaaaa
      661 aagccatcct gtaggacggc tttgattatt tttacttaaa cagtattaat attcgtcttc
      721 attaaagaag aaatcatctc ttcgtggata gtctggccat atatcttcaa ttgattcata
      781 gatatcaccg tcatcttcca tttgctgaag attctctatt acttcaagcg gtgttcctga
      841 acgtatggcg aagtcgatga gttcatctct ggtagccggc catggtgcat cttcgaggtg
      901 tgaggctagt tcaagtgtcc aatacataaa acgatattat ttaaataatt atttataatg
      961 ttattgcctg caaacgtact gaatctaaaa aaagttccga aaaaattgaa tatttattaa
     1021 caatattaaa ttatcatcat tgcatcaccg tagctgtaaa accgatactt ttcttttatc
     1081 gcttcgtgat atgcttccat tgttagttcg taccctgcaa atgcagcaac catgatgatc
     1141 agacttgatt ttggtaaatg aaaattagta atcatcgaat tggcgatact gaaatcatag
     1201 ggtggataaa taaatttgtt cgtccaacct tctgcagcct tcagaaaacc ttcagcagat
     1261 acggatgact ctatggcacg catacttgtt gtaccgacag cacacactcg gcgaccagct
     1321 tcttttgcat cgttgacata ttttgctgcc ttctcaggaa tcctgaaata ctcggcatcc
     1381 attttatgtt ttgacagatc ttctacatca atggttctga atgtacccaa gccaacgtgc
     1441 aaagtaagct ctgcgaaatt cacgcctttg agttcgagac gtttcatcag ttcttggcta
     1501 aagtggagtc cggcagtcgg tgcagctaca gcgcccaatt ctttggcgta tatggtttgg
     1561 taccgttcct tgtcaaatgt atccgcatct cggttgatgt atttaggcag tggtgtattg
     1621 ccaagataat caagatcggc attaaactga tcgtctgtac cgtcataaag aaagcgaatg
     1681 gttctgccac gacttgtagt gttgtccact acttctgcga cgagtatgtc gttgttcttg
     1741 tcgtcggtaa aatagagttt attacctact ctgatttttc ttgccgggtc aacaagtacg
     1801 tcccatagtc tttgttcgtg attaagctcg cgcaatagga atacttcgat atcagctcct
     1861 gttttttcct ttttgccata caatcgggca gg
//
LOCUS       DAOQQY010000404         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_37211_length_1886_cov_3.647187, whole genome shotgun sequence.
ACCESSION   DAOQQY010000404 DAOQQY010000000
VERSION     DAOQQY010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37211_length_1886_cov_3.647187"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="PLE23_12915"
     CDS             complement(<1..833)
                     /locus_tag="PLE23_12915"
                     /inference="COORDINATES: protein motif:HMM:NF014708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMX84215.1"
                     /translation="MNTKSVEAIIQPREPHFVGDGFSVHNFIPASYLLDMQRMNLFIL
                     LDYGSKYHFDPTEIPKGVGVHPHRGFETVTIAYKGRIEHHDSSGGGGIIGEGDVQWMT
                     AASGILHKEFHESEWSKTGGVMQMVQLWVNLPAKDKMSKPKYQALKKEAISRYILDDG
                     KGEIEVIAGEYMGMKGSASTFTPMYVMNARLKADAEAKFRLPAHYNTALLVIEGSTRV
                     NEGENVPTDNFVLMARDGEDFVINANEDAVVLILSGEPIHEPIAAHGPFVMNTRQELM
                     EA"
     gene            1080..1811
                     /locus_tag="PLE23_12920"
     CDS             1080..1811
                     /locus_tag="PLE23_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84216.1"
                     /translation="MTEMKQFVPTDEAKGQAKQLRLFAILAWVIAIAGEIFAIMKLLH
                     NDMLTWLIVAIVVILGLSIAGSLMWKKANRLDPASEKDKTRFFFQNQLGAIMAVLAFL
                     PLVIFIFTNKDLDKKTKGIAGTIGIIALLVAGITGIDFNPPSVEKYTQQINEQTQVIK
                     DLNNGLDLVYWTDQGNKFHIFEDCQHIKGRNIHSGTVEDAWKARKIGDSELCLTCRKR
                     SEKAHGLEPGSTLKEVPEAAPQEAQ"
BASE COUNT          501 a          476 c          376 g          533 t
ORIGIN      
        1 gcctccatca gttcttgtct tgtgttcatg acgaatggcc catgtgcggc tattggctca
       61 tggatgggtt caccgcttaa tattaaaaca acggcgtctt catttgcatt tatgacgaag
      121 tcttcaccat cccgtgccat cagtacaaag ttatcggttg gtacattttc accttcgtta
      181 acccttgtgc tgccttctat taccagcaat gccgtattgt agtgtgccgg caaacgaaat
      241 ttcgcttcgg cgtctgcctt cagccttgca ttcattacat acataggtgt aaaggtagat
      301 gcacttcctt tcatacccat gtattctcct gctattactt ctatttctcc tttgccatca
      361 tctagtatat agcggcttat tgcttccttt ttcagagcct ggtatttggg tttgctcatc
      421 ttatccttcg ccggtaggtt tacccacagc tgcaccatct gcataacacc tccggtctta
      481 ctccactcgc tctcgtgaaa ttccttgtgc agtatgccgc tcgcagcagt catccactgt
      541 acgtcacctt cgcctatgat gccaccgcca ccactactgt catggtgttc gatgcggcct
      601 ttgtatgcaa tggtcacagt ctcaaatcca cgatgaggat gcacacctac accttttggt
      661 atctcagtag ggtcgaagtg atatttcgac ccgtaatcta ataatatgaa cagattcatg
      721 cgctgcatat caagcaagta tgaggccggt atgaaattat gtacactgaa tccgtcacct
      781 acgaagtgcg gctcacgtgg ctggatgata gcttctacag attttgtatt catgatgttt
      841 ttttatacaa atttacgttg agttcaactg cattgcctta atgtacatta agaagtacga
      901 tatgtatcat ataaggtatt atattactgc ctgatcacaa atgttttatt gttgatggaa
      961 ttatttcaac gctttatttg ttgcttggta gcaaaccgga tatttcgaga caaaattgta
     1021 caaaatttca cgtgtttgtt taaatttgta acattattcc aattcaaaaa atcataacca
     1081 tgacggaaat gaaacagttc gtaccaacag atgaagcaaa agggcaagca aaacaactcc
     1141 ggctctttgc tatactcgcc tgggttattg ccatagccgg tgaaatattt gccatcatga
     1201 aactcctgca caacgatatg ctcacttggc tcatcgtagc cattgtggtc atcttggggc
     1261 tatcaatagc aggctcgctc atgtggaaaa aagccaaccg actcgatccg gcatccgaaa
     1321 aagacaaaac acgcttcttt tttcaaaacc aactgggcgc catcatggct gttcttgcat
     1381 ttctgccgct ggtcatcttc atattcacca acaaagacct cgacaaaaag accaaaggca
     1441 tagcaggtac catcggcatc attgcgctgc tcgtcgctgg tatcacaggc atagacttca
     1501 atccgccatc cgtagagaaa tatacacagc agatcaacga gcagacacag gtcatcaaag
     1561 acctcaacaa tggcctcgac ctcgtttact ggaccgacca aggcaacaaa tttcacatat
     1621 tcgaagattg ccagcatatt aaaggccgca acatacacag cggcacagta gaagatgcct
     1681 ggaaagcccg caaaataggt gactccgagc tctgcctcac ttgccgcaaa cgctctgaga
     1741 aggcacatgg cctcgaaccg ggttctacac tcaaagaagt acccgaagca gcaccacaag
     1801 aggcacaata acgctgtaaa atgaatgttt tgggcgtgcc cctgtcgggt caggccatcc
     1861 gctttacttc gtgttcgctt ctgtcc
//
LOCUS       DAOQQY010000405         1886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_37215_length_1886_cov_3.569088, whole genome shotgun sequence.
ACCESSION   DAOQQY010000405 DAOQQY010000000
VERSION     DAOQQY010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37215_length_1886_cov_3.569088"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..>1886)
                     /locus_tag="PLE23_12925"
     CDS             complement(211..>1886)
                     /locus_tag="PLE23_12925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HMX84217.1"
                     /translation="LLMKKYLLNGVDKKSGMQDKYLSGGRANAYNSITELLKQCSSCL
                     PPLNINSIVISGTEANITWSLPDSVSEVKLNYKGPGSTWKTIDHLNGTNYIIQNLQPC
                     TVYEFQLASYCNDTLSSYSNTLSFKTLGCCEGPFGLSVTKTDTTANISWDAVFGSDAY
                     ILFYKKETDTDFSKIQTKSSPQTISGLEPCTLYQIFVSTQCANGITTNSDTISFKTDG
                     CGACESLVYCDAFGSEYQEEWISNVSLNGTSNTSENDGYKFFIETSFNVEKNKPSTIS
                     ITPSYAGAFYSEHFRVWIDYNQDGSFDDVSELVFDPVDTNTTVTGSFVVPSTAVAGHT
                     RMRVAMSFASFGGDKPEACGEIVFGEVEDYCVFIKDTVVTPQSCDAPTGLEVPELGKT
                     NVKLNWNKVALAGKYTLEYKLASDTIWEQLPNILEPPIAIGGLIADTTYVWRVQSNCD
                     VDNSSFSVVDTFTTAKPDATTYIGADRIFSLFPNPVQGQVSIFFREAGLSNISISVRT
                     VNGTELLHTQNESVTPAEIRLDISHLVAGMYFITIQSDQGTYVQKLIKQ"
BASE COUNT          538 a          369 c          383 g          596 t
ORIGIN      
        1 atgaatgtaa atacaaggca aaatagaaga agatttttca tcagcaataa ttgtgatatc
       61 tgatatctgc tacaatttta cgaaaaaaat accgcttgaa attagatttt tcacactttt
      121 tccttggagc aaaaaaaatt aaataaaaaa cagcacctgc cattgagttg gcgagatgct
      181 gtttctggtt tctaatacaa taatgtgctt ttattgtttt atcaattttt gtacgtatgt
      241 accttggtca gattgtattg taataaaata catgcctgca accaaatgtg aaatatcaag
      301 tcttatttcg gctggggtga cactctcgtt ttgggtatgc aataattctg taccattaac
      361 ggtacggact gatatagaaa tattactcag ccctgcttct ctaaagaaaa tacttacttg
      421 gccttgtacc gggttaggga ataggctgaa aattcggtct gcaccgatgt aagtagtcgc
      481 atccggcttg gcagttgtga aagtatccac gactgagaat gatgaattat ccacatcgca
      541 attgctctgc actctccaga cataggtcgt atctgcaatg agtccgccta tagcaatagg
      601 aggttcaaga atgtttggta actgttccca aatggtgtct gaggccagct tgtattcaag
      661 cgtatatttg ccggctagtg ctactttgtt ccagttgagt ttgacgttgg ttttgccgag
      721 ttccggcact tcgagtccgg taggagcatc acaggattgt ggcgtgacta ctgtatcttt
      781 gataaaaaca caataatctt ctacttcacc aaatactatt tcaccacaag cttcaggttt
      841 atcgccgccg aagctggcaa aactcatcgc tacacgcatt ctggtatgcc ccgcaacagc
      901 agttgaaggc acaacaaagc ttcctgtaac ggtagtattt gtatcaacag ggtcgaaaac
      961 caattctgaa acatcatcaa atgatccgtc ctgattgtaa tcaatccata ctctgaagtg
     1021 ctcagaataa aatgcaccag cgtatgacgg tgtaatgctg atggtagaag gcttattttt
     1081 ttcaacatta aaggatgttt caataaaaaa tttgtaaccg tcattttctg aagtattgga
     1141 tgttccgttt aagcttacgt tgctaatcca ttcttcctga tattccgacc caaatgcatc
     1201 acagtaaaca agtgactcgc aagcaccaca tccgtcggtc ttgaagctga tggtatcact
     1261 gttggtagtt attccgttgg cgcactgggt gcttacgaag atttggtaaa gtgtacacgg
     1321 ttcgagccct gaaatggttt gtggcgagga cttggtttgt atttttgaaa aatctgtatc
     1381 tgtttctttt ttgtaaaata atatgtatgc atcgctgcca aatacagcat cccagcttat
     1441 gttcgctgta gtgtctgttt tcgttacgga taaaccgaac ggaccttcgc aacatcctaa
     1501 agttttgaaa ctaagtgtat tagaatatga actcagtgtg tcattacaat aagatgccag
     1561 ctgaaattcg taaaccgtac acggctgaag attttgaata atataatttg tgccattaag
     1621 gtgatctatt gttttccatg tagatcccgg acccttataa tttaacttaa cttctgatac
     1681 tgagtcagga agtgaccagg taatatttgc ctctgtccct gaaatgacaa tactgtttat
     1741 gttcaaaggt ggcagacacg aactgcattg tttgagcaat tctgtgatgc tgttataggc
     1801 attcgcccga cctccggata ggtatttgtc ttgcataccg ctctttttgt caacaccatt
     1861 caacaaatat tttttcatga gaaggg
//
LOCUS       DAOQQY010000406         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_37410_length_1880_cov_5.337534, whole genome shotgun sequence.
ACCESSION   DAOQQY010000406 DAOQQY010000000
VERSION     DAOQQY010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37410_length_1880_cov_5.337534"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..746)
                     /locus_tag="PLE23_12930"
     CDS             complement(141..746)
                     /locus_tag="PLE23_12930"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMX84218.1"
                     /translation="MKANTLLIFGALCLLLSLASFTKPPVGYKIGDKVADFSLKGVDG
                     KNHSLAEFKQAKGYIIVFTCNECPYAQMYEQRIIQLHNKYSKLGYPVLAINPSLVDYF
                     PEESYDEMVKRSKASKFTFHYLFDKNQGVTKLFRPSKTPHVFLLDKNRVVKYIGAIDD
                     NPQSAAAVKKKYVESAIEALNKGKNPTPAETKAIGCAIKIN"
     gene            complement(761..1294)
                     /locus_tag="PLE23_12935"
     CDS             complement(761..1294)
                     /locus_tag="PLE23_12935"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMX84219.1"
                     /translation="MKSLKFFSFLLLLSLFACTGTTEVKRDNTTVATASADAKPEILT
                     SFDQLNDRYFQVKDSLVIINFWATWCKPCVRELPDFNEAALKYKGQKVKFVFVSLDFL
                     DTMEKSIVPFIKSNPFNGEVVVLADQDVNTWAINIDNNWDGAIPVTLAIKNGKKSTKM
                     GAFQNYEELNNFIQQNI"
     gene            complement(1327..>1880)
                     /locus_tag="PLE23_12940"
     CDS             complement(1327..>1880)
                     /locus_tag="PLE23_12940"
                     /inference="COORDINATES: protein motif:HMM:NF013499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HMX84220.1"
                     /translation="EVENFTNTSLANIEGKNIPKIKFAAIVTAASFGTDKKGYQMGNF
                     VLQDYTGSFNLRLFSGDFEKFGQYFKYGNAVFVEGHWKARFKGDGQVFNLTNVSMLSD
                     VAGNMTETITIRLDTSQINAEQIVKLDKIFSRKTGKQKLRFTLHNPLENISLQMSSTE
                     RKVTIDTHFLSELDKLGYEFSLS"
BASE COUNT          528 a          349 c          369 g          634 t
ORIGIN      
        1 catgctgcaa tgataaggat tctgtagcgt ttacagaagt aatagtacgt caattttgta
       61 catcatgcaa ctgtcttcag agatttttta accaaaaacc acaagcagct atacacctta
      121 caaggcaatc agataatctg tcagttgatt ttgatggcac aaccgatggc tttggtttca
      181 gcaggtgttg gattttttcc tttgttgagt gcttcaatgg ccgattcgac atattttttc
      241 ttaacagcgg cagcactttg cggattatca tcaatagcgc ctatgtattt taccactctg
      301 tttttatcaa gtaggaatac atgtggtgtt ttcgaaggtc tgaaaagttt ggttactcct
      361 tggtttttgt caaaaagata atgaaatgta aatttgcttg cctttgagcg ttttaccatc
      421 tcgtcatagc tttcttcagg gaaataatcc accaaactcg gattaatagc aagtaccgga
      481 taaccgagtt tgctgtattt gttgtgaagt tgaatgattc tttgctcgta catctgtgca
      541 taaggacatt cattacaggt aaatacgatg atgtagcctt tcgcttgttt gaattcggcc
      601 agggagtgat ttttaccgtc aacacctttg agtgaaaaat ccgctacctt gtcaccaatt
      661 ttgtatccga caggtggttt tgtgaaggaa gcaagactta aaagcaggca caatgctcca
      721 aaaatcagta atgtattagc cttcatagta tcttggtgcg ttaaatattt tgttgaatga
      781 agttgttcaa ttcctcataa ttttggaatg cacccatttt tgtcgatttc ttaccatttt
      841 tgatggctaa ggtcaccgga atggcaccgt cccagttgtt gtcaatgtta atggcccaag
      901 tatttacatc ctggtcagca agcacgacca cttcgccgtt gaacgggttg gattttataa
      961 aaggaacaat acttttttcc attgtatcaa ggaaatcaag gctaacgaat acgaatttta
     1021 ccttttgccc cttgtacttt aatgctgctt cgttaaagtc aggaagttct cgtacacaag
     1081 gtttgcacca agtagcccaa aaattgatga tgacaagtga gtctttcacc tgaaaatatc
     1141 tgtcattcaa ctgatcaaag gatgtaagaa tttcaggttt tgcatcagca gatgccgtag
     1201 ctacggtagt attgtccctt ttgacctcag tagtacctgt acaggcaaat aggctgagta
     1261 ataagagaaa tgagaaaaat ttaagtgatt tcatagtaat aaaaggttaa aagattcaat
     1321 aaatcatcag cttagtgaaa attcgtagcc aagtttgtct agttctgaga gaaagtgtgt
     1381 atcaattgtt acttttctct cagtggacga catctgtagc gaaatattct ctagcggatt
     1441 gtgtaatgta aaacgcaatt tttgctttcc ggtttttctg gaaaagattt tatctagttt
     1501 tacaatttgt tcagcattta tctgtgaagt atcgagcctt atggtaatgg tttcggtcat
     1561 attgcccgcc acatctgata acatggacac atttgtcagg ttaaatactt gtccgtcacc
     1621 tttaaatctg gctttccagt gtccttccac aaataccgca tttccgtatt taaagtattg
     1681 cccaaatttt tcaaaatcac cgctgaataa tcgcaggttg aacgaaccgg tgtagtcctg
     1741 aagtacaaaa ttacccattt ggtacccttt tttatcggtt ccgaaactgg cagctgtaac
     1801 gatggcggca aattttattt ttggaatatt ctttccttca atattcgcaa ggcttgtgtt
     1861 ggtaaaattt tctacttcag
//
LOCUS       DAOQQY010000407         1844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_38716_length_1844_cov_6.339296, whole genome shotgun sequence.
ACCESSION   DAOQQY010000407 DAOQQY010000000
VERSION     DAOQQY010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38716_length_1844_cov_6.339296"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="PLE23_12945"
     CDS             complement(<1..236)
                     /locus_tag="PLE23_12945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84221.1"
                     /translation="MKKDSELERMLRDQQQHLAKLKEIVVETINEEKLIINNLMNPQA
                     DILTRGQRISDKVARFGGSWKFIIAFGVILFLWIV"
     gene            651..1289
                     /locus_tag="PLE23_12950"
     CDS             651..1289
                     /locus_tag="PLE23_12950"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMX84222.1"
                     /translation="MAGDFFVTTIGYFPSVEVFVLGENYSKWKVEAQEHYQKTGLRNK
                     CCIVGSQGRCTLSIPLEQGRNNGLPIREVKISNTVNWQKQHWQTIRTNYGKSPFFIHY
                     APYFEAFYQKGHQYLFDLCIESTALLIKLLKLDVSLEPTKDFIRHYEGEGILDFRFPK
                     TANSFVKIIHQPYPQVFSDRHGFTENLSAIDILFCLGTDAKKILKKSKIMYL"
     gene            1382..>1844
                     /locus_tag="PLE23_12955"
     CDS             1382..>1844
                     /locus_tag="PLE23_12955"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HMX84223.1"
                     /translation="MTRIFCVLLNFVVIFNLTGQNKPNPVIKVKYPKTKTVNQVDDYF
                     GTKVSDPYRWLEDDKAPEVAQWVDEENAVTASYFSNIPFRSKIVSRYQELFNYVKLST
                     PIKIGKYFIFSKNEGLQNQPVVYIQEGEKGEPQVLLDPNTMSKEGTEYITLM"
BASE COUNT          620 a          317 c          343 g          564 t
ORIGIN      
        1 actatccata aaaacaaaat gaccccgaat gcaataatga atttccaact acctccaaac
       61 cgtgcaactt tgtcagaaat gcgttgacca cgtgtgagta tatcggcctg tggattcatc
      121 aggttgttga tgatgagttt ttcttcattg atggtttcga caactatctc ttttagtttt
      181 gccaggtgct gttgttgatc tcttagcatt ctttcaagtt cggagtcttt tttcatatta
      241 aaatgttttg tgcaaacaaa attaagtaaa gtgccggaat aaaccgataa cactgtgcag
      301 aatattcaaa taagagaatt gtggttgtcg gtatcacaca catttttaaa taacagcaat
      361 gaaaaacttt ttttcggcaa atgaatgaaa ttttccgaaa ttctttcaat tgttaataaa
      421 aatcactaca ttgcagaatc aaataaaagt attattatta tgtcacaatc taggaaagct
      481 cagaatatac agaggaaaga tcagagagat tcgaagaaat tttttaccat actcggcatt
      541 atcgtagttg tattgatgct ggtagtgtac ttgatatatt ctaacagtta atactgagtt
      601 tacatcatca gaattaattc cagtatttct ttttaccata ataacatgat atggcaggag
      661 atttttttgt cacaacaatt ggctatttcc catctgttga agtgtttgta ttaggtgaaa
      721 attattcgaa atggaaggta gaggcacaag agcattatca gaagaccggg ttacggaaca
      781 aatgctgtat tgttggttcg caaggtaggt gcaccttgag tattccgctt gagcaaggca
      841 gaaacaatgg gttgcctata cgtgaagtaa aaatatccaa tactgtcaat tggcagaaac
      901 agcattggca aaccatccgt accaactatg gcaagtcgcc tttctttatt cattatgctc
      961 cttattttga agctttttac caaaaaggac atcaatactt gttcgaccta tgtatagaaa
     1021 gtactgcctt gctgatcaaa ctactcaaac tggatgtaag tctggagcca acaaaggatt
     1081 ttatccggca ctatgaagga gaaggtatct tggattttag gttcccaaaa acggcgaatt
     1141 catttgtaaa aataatacat caaccttatc cgcaggtatt cagtgaccgg catggcttta
     1201 cagaaaatct ctcagccatt gatatattat tttgtttagg aactgatgct aaaaaaattt
     1261 tgaaaaaaag taaaattatg tatctttgac gttattgggc tgtataggga aaagtgcaat
     1321 aggaatacgc cgcaaacaat agattttagc taatatttta tgaaccataa aacgatccgt
     1381 catgactaga attttttgcg tcttattaaa cttcgttgtc attttcaatc ttacaggtca
     1441 gaacaaacca aaccctgtga taaaagtgaa atatcctaaa acaaaaaccg taaaccaggt
     1501 tgatgattat tttggcacca aagtatcaga tccatatagg tggcttgaag acgacaaagc
     1561 tcccgaggta gcacaatggg tagatgaaga aaacgccgtt acagcttcgt atttttcaaa
     1621 cattcctttc agaagcaaga ttgtatccag atatcaggaa ttattcaatt atgtaaaact
     1681 atcgacacct atcaagattg ggaagtattt tattttttcg aaaaatgaag gccttcaaaa
     1741 ccagccggta gtttacattc aggaaggaga aaaaggcgaa cctcaggtat tacttgaccc
     1801 aaatactatg tcaaaggagg gcaccgaata catcacattg atgg
//
LOCUS       DAOQQY010000408         1818 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_39703_length_1818_cov_5.614294, whole genome shotgun sequence.
ACCESSION   DAOQQY010000408 DAOQQY010000000
VERSION     DAOQQY010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39703_length_1818_cov_5.614294"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLE23_12960"
     CDS             <1..470
                     /locus_tag="PLE23_12960"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMX84224.1"
                     /translation="YSALDYLIKPVEIEELSQAIDKAIQKRESSSNQRLELLLENLRH
                     DSKEIRKIAISSPEGLQFVRLENLLYLEATGNYTHVHTCDKKKYLSSHSLKIFEEILP
                     VHQFLRIHNSYIINLDYVVKYIRGEGGKVVLEGNVTLDISKRKKADFLKAIRM"
     gene            complement(615..1157)
                     /locus_tag="PLE23_12965"
     CDS             complement(615..1157)
                     /locus_tag="PLE23_12965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HMX84225.1"
                     /translation="MGGFENLPDPVPASQMIYNDVARFFDNSKTNFVAHKTGDAYTYN
                     YSINIPADWEKDSLTIVAVLLNNNGIVSNANDATIAQAIQNGYIVATNSPITQSDIKI
                     YPNPATGSTNIALKLTDPTPVNISIQDMLGRVQYTENHELLQGEINLPINLTLLTPGT
                     YQVLLKTESGTATSKLIIVR"
     gene            complement(1109..>1818)
                     /locus_tag="PLE23_12970"
     CDS             complement(1109..>1818)
                     /locus_tag="PLE23_12970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84226.1"
                     /translation="HLDSLDIEPGNYGEITSAITLSLPDGLDTVFMELVSINGKADFV
                     ECNNFSSNYVFGANPSPNRKVILESSASTTNGASPVSYSALQNCRTFYNGYYIPIAVH
                     FDDPMAVPAYQNSLAPYISAENIPQCMVDRDYVADITNPDGILGISLDYLSKEPDALI
                     NIGAKYGTDTSQLKVSVTLDFTKDVPENYSAYLILKENGVHKNDPGFDQANYFPTMLM
                     AQWVGSKTFLTLCLPHK"
BASE COUNT          539 a          358 c          384 g          537 t
ORIGIN      
        1 aatacagcgc ccttgattac cttataaaac ctgtggagat tgaagaactc agtcaagcaa
       61 tcgacaaggc tatacaaaaa agagaaagct cttccaacca aagactagaa ttactgcttg
      121 aaaacttaag gcacgatagc aaagaaataa ggaagatagc catttcctca ccagaaggtt
      181 tgcagtttgt gcgactggaa aatctgcttt accttgaggc caccggcaat tatacccatg
      241 tacatacctg cgacaagaaa aagtatcttt caagccatag ccttaagatt tttgaagaaa
      301 tactgcccgt acatcagttc ttgcgaatac ataattcgta cataattaat ctggactacg
      361 ttgtaaaata tatacgagga gaagggggca aggtagtatt ggaaggcaat gttacccttg
      421 atatttcaaa aagaaagaaa gcagatttct tgaaagcaat caggatgtaa ttgaacccag
      481 ctaactacgc gaagatttgt ttgtatcgca cataaaaatg tctccgacca gatacccagc
      541 cggagaccta aatcattcaa gcatccaaat ttatttatga aaataaaatt gttcgtttat
      601 aaaaccacct gcattcatct gacaatgatc agtttgctgg tagcagtgcc agactcagtt
      661 tttaacaata cctggtaagt acccggagtt aatagggtca agtttatcgg taagttaatt
      721 tctccttgca acagctcgtg gttttcggtg tattgtactc ttccaagcat atcctgaatg
      781 ctgatattca ccggggtagg gtcggttaac ttcaatgcta tgttcgtact gccggtagcc
      841 gggtttggat aaatcttgat gtcggactgg gtgattgggc tattggttgc gacgatatag
      901 ccgttctgga tggcttgtgc aatggtagcg tcattggcgt tagatacaat tccattatta
      961 ttcagtaata cggctactat tgttaaactg tccttttccc agtcagcagg gatatttatt
     1021 gaataattat aagtgtaagc gtctcccgtt ttgtgcgcca caaaatttgt tttactattg
     1081 tcaaaaaacc gtgcaacatc attatagatc atttgtgagg caggcacagg gtcaggaagg
     1141 ttttcgaacc cacccattgg gccataagca ttgttggaaa ataatttgcc tgatcaaaac
     1201 cagggtcatt tttgtgtacg ccattttctt tcagaatgag ataagcactg tagttttctg
     1261 ggacgtcttt tgtaaaatca agtgtgacag atacctttag ctggctggta tcggttccat
     1321 acttggctcc tatattgatg agggcatctg gttctttgct tagataatca agagaaatac
     1381 ctagaatacc gtccggattg gtgatgtcgg caacatagtc tctgtcaacc atacactggg
     1441 gtatattctc tgctgaaata tacggtgcca aagaattctg atatgccggt actgccatcg
     1501 ggtcatcaaa atgtaccgct atcggtatgt agtaaccatt gtaaaaagtt ctgcaatttt
     1561 gcaatgcact gtaactaaca ggcgatgcac catttgttgt actcgcactt gactctaata
     1621 tcaccttacg gttaggggat gggtttgcac cgaacacata attggatgaa aaattattgc
     1681 attctacaaa atcagctttt ccgttaatgc tcaccaattc catgaaaaca gtgtccagcc
     1741 catccggaag actgagtgta atggctgagg taatctcacc gtaatttccg ggttctatat
     1801 cgaggctgtc aagatgtt
//
LOCUS       DAOQQY010000409         1809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_40035_length_1809_cov_3.900798, whole genome shotgun sequence.
ACCESSION   DAOQQY010000409 DAOQQY010000000
VERSION     DAOQQY010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40035_length_1809_cov_3.900798"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="PLE23_12975"
     CDS             <1..267
                     /locus_tag="PLE23_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84227.1"
                     /translation="DAKIPLVYVNRKPANLPEGVAYVGSDSIDAGHFQMEYIAKKLDF
                     SLEEFEELLALPNRDHAEFGDEQKTEAVINSVRRIFRPIKRILK"
     gene            267..1319
                     /gene="wecB"
                     /locus_tag="PLE23_12980"
     CDS             267..1319
                     /gene="wecB"
                     /locus_tag="PLE23_12980"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMX84228.1"
                     /translation="MDKTKIAVVIGARPQFIKHAALEHELRKSFNVITIHTGQHYDEN
                     MSQVFFDQLGMARPDYILNIGSAGHGVQTAKMMIEIEDILLKERPAYLLVYGDTNSTL
                     AGALVAAKLQVKVIHVEAGLRSYNREMPEEINRVLCDHVSDLLFCPTHAAADNLKAEG
                     ITKNVYVVGDIMTDMQQIALDRKMVSKTAEAESYYFATIHRPYNTDHKDRLLTIIETF
                     QKLDKPVRFSVHPRTMHKLNEWHCDLKLYKNVTFLEPLSYFDSLNMQYNAAAVITDSG
                     GMQKEAYFFRKKCVTIRKETEWTETLVGNWNTLVFDNLDELQIALRLVPTNYVEDLYG
                     NGNTAVKIVETVSGYK"
     gene            1367..>1809
                     /locus_tag="PLE23_12985"
     CDS             1367..>1809
                     /locus_tag="PLE23_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84229.1"
                     /translation="MESKKQNNNSINILFLIAEFAPVNTTGNFRSLKFVKYLPDLGIN
                     PVVITLPAEEGASLFNAKLDNRLLDEIPPSVEIYRISPEVEFNKKENKLKSYIKHFLR
                     FGSDGLGKYWIKKLSPALKDIIRKHDIEAIYTTLPPFSMGMVARS"
BASE COUNT          602 a          343 c          393 g          471 t
ORIGIN      
        1 gacgccaaga ttccgttggt ctatgtgaac cgcaaacctg ccaatctgcc ggaaggcgtc
       61 gcctatgtcg gttcagactc gattgacgct ggtcacttcc agatggaata tattgccaag
      121 aaactggatt tttcattaga agagttcgaa gaattacttg ctttgcccaa cagagatcat
      181 gcagagtttg gtgacgagca aaaaacagag gctgttatta attcggtgcg aagaattttc
      241 cgaccaatta aaagaatatt aaaataatgg ataaaacaaa aatagccgtc gtcatcggag
      301 cccggccaca atttattaaa cacgctgcgc tcgagcatga gttgaggaag agttttaatg
      361 tgataaccat tcacacaggt cagcattatg acgaaaacat gagtcaggta ttctttgacc
      421 agctgggaat ggctcgtcct gactatattt tgaatattgg aagtgctggt cacggtgttc
      481 aaacagcaaa aatgatgata gaaatagaag atattttgct caaagaacga ccagcttatc
      541 ttcttgttta tggcgataca aatagtacct tggcaggtgc tttggtggct gccaaattac
      601 aagtaaaagt gatacacgta gaagcaggct tgcgcagtta caatcgtgag atgcccgaag
      661 agattaaccg ggtcttgtgt gatcatgtat ccgatctgtt gttctgccct acacatgcgg
      721 cagcagataa tctgaaagca gaaggaatta caaaaaatgt ttatgtagta ggagatataa
      781 tgactgatat gcagcagatt gccctggaca gaaaaatggt gtccaagact gccgaagcag
      841 aaagctatta tttcgccacc attcaccgac cgtacaatac cgaccacaaa gacagactat
      901 tgaccataat tgaaaccttc caaaagctag ataagccggt caggttttct gtccatccgc
      961 gaacgatgca taaactaaat gaatggcatt gcgatctgaa gctatataag aacgttacat
     1021 ttctggagcc gctttcctat tttgacagcc tgaatatgca gtacaatgct gcagccgtca
     1081 tcaccgattc gggcggaatg cagaaggagg cgtatttttt cagaaaaaaa tgtgttacaa
     1141 tccgtaaaga aacagaatgg accgaaacgc ttgttggtaa ttggaataca ctggtttttg
     1201 ataatctgga tgaattacag atcgcattga ggcttgtacc aacaaattat gtagaagacc
     1261 tatatggaaa tggtaataca gcagtgaaga tagttgaaac cgtatcagga tataaataag
     1321 aaacagggac agcagtatga gcaaactctt tacaaataca gaaccgatgg agagtaaaaa
     1381 acaaaataat aattccatca atattttatt cctgattgca gaatttgcac ctgtaaacac
     1441 aaccgggaat ttcaggtcgc tcaaatttgt aaaatatctt ccggatttag gtatcaatcc
     1501 ggtagtaatt actctgcctg ccgaagaagg tgccagtctg ttcaatgcaa aactagataa
     1561 caggctttta gatgaaatac cgccctccgt agaaatatat cggataagcc ctgaagtaga
     1621 attcaataaa aaagaaaaca agttaaagtc atacatcaag cattttttga gatttggttc
     1681 agatggtctt ggaaaatact ggatcaagaa actgtcgcca gctttaaaag acattatcag
     1741 gaagcatgat atagaggcca tatatacaac gctcccgcct tttagtatgg gcatggttgc
     1801 acgctctat
//
LOCUS       DAOQQY010000410         1798 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_40441_length_1798_cov_5.986231, whole genome shotgun sequence.
ACCESSION   DAOQQY010000410 DAOQQY010000000
VERSION     DAOQQY010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40441_length_1798_cov_5.986231"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            226..299
                     /locus_tag="PLE23_12990"
     tRNA            226..299
                     /locus_tag="PLE23_12990"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:260..262,aa:Asp,seq:gtc)
     gene            573..1424
                     /locus_tag="PLE23_12995"
     CDS             573..1424
                     /locus_tag="PLE23_12995"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMX84230.1"
                     /translation="MNEKYFTPLSGYLMFFVMLIVIAFGIYAAVALENFVITALCGLL
                     TFFMLNGFFLINPNNSRVLTLFGEYKGSAKQNGFFWVNPFMTKKVVSLRASNFESDRM
                     KVNDKRGNPIEISVILVWRVADTFKAAFEVDDYVKFVKVQTDAAVRKLAHLYPYDNAD
                     DTNELTLRGGTEEINHSLEEEIRERLNISGIEVLEARIGYLAYASEIAGAMLKRQQAE
                     AVVAARYKIVEGAVGMVEQALEEMSKRSIIDLDDDKKAAMVSNLMVVLCGDREANPVI
                     NTGTLNS"
     gene            1426..1605
                     /locus_tag="PLE23_13000"
     CDS             1426..1605
                     /locus_tag="PLE23_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84231.1"
                     /translation="MARKQIVLRIEEETLRAIEKWAADEFRSANGQLEWMLHDCLRRT
                     KRLPKKSEATKHKPE"
BASE COUNT          453 a          429 c          404 g          512 t
ORIGIN      
        1 ggtcatagcc atggcagtgg atgtctttta aattggttct aaccggcgcc cttcgaagga
       61 ggcctttggt atcatcgcgc ttcccgggga gtgcaaaggt atggtgtggg cggctgattt
      121 tgtgccgtct gtatgcccag catttttttt aaaaatttat tctgaaaaat ttgttgatcc
      181 aaaatttgtg cgcttatctt tgccccgctt tagaaatgaa gcgctggatt ggtagttcag
      241 tcggttagaa tgccgccctg tcacggcgga ggtcgcgggt tcgagtcccg tccagtccgc
      301 aaaagccctc ggagatttcc gggggctttt cttttttaac aattttttcc tccacatctc
      361 ggattattct gcatcgatca gttgctcctt gcattccgct ttttgtcatt ctgtccattt
      421 ttttctgtct ggtctcttca tttatcggtg cacgaatgcc gtatttgctg catttctgcc
      481 ataaatttta cccaaaatta aaaataatca gccttttact tgcaagtatg attcgcataa
      541 tgatatattt gtgatatcaa aacgatatta ttatgaatga aaagtatttt accccgcttt
      601 caggttacct gatgtttttt gtaatgctca tcgtcattgc ctttggcata tacgctgctg
      661 tagctttgga aaattttgtc atcacagccc tttgtggttt gctcactttc tttatgttga
      721 atggtttttt tctcatcaat ccgaataact caagagttct taccctgttc ggtgagtata
      781 aaggctctgc caaacagaat ggcttcttct gggtcaatcc ttttatgacg aaaaaggtgg
      841 tttcgctccg tgcatccaac ttcgagagcg accgcatgaa ggtaaatgac aaacgcggaa
      901 accctatcga gattagtgtg atactcgtgt ggcgcgtagc cgatacattc aaggcagctt
      961 ttgaagtgga cgattatgta aaattcgtca aagtccagac cgatgccgct gtcagaaaac
     1021 ttgctcacct gtacccttat gacaatgccg atgacacaaa cgaacttacc ctgcgtggcg
     1081 gcacagaaga gattaaccac agcctcgaag aagaaatccg cgaacgactc aacataagtg
     1141 gcatcgaagt gctcgaagcc cgcatcggtt atctggccta tgcttctgag atagcaggtg
     1201 ccatgctcaa acgtcaacag gccgaggccg tcgtagctgc acgctataaa atcgtagaag
     1261 gagccgtagg tatggtcgaa caagctcttg aagaaatgtc aaaacgttct atcattgatc
     1321 tggacgatga caagaaagca gctatggtca gcaacctgat ggtcgtactc tgcggcgacc
     1381 gcgaggccaa tccggtaatc aacactggta cgctcaattc ttaagatggc acgcaaacaa
     1441 atagtactcc ggatagaaga agaaacactt agagccatcg aaaaatgggc tgcagatgag
     1501 ttccgctccg ccaatggcca actggagtgg atgctgcatg actgcctgcg tcgaacgaag
     1561 aggctaccca aaaaatctga agctacaaaa cacaaacccg aataaatgcc tccctcaagc
     1621 atacggctct gctccacagc agggccgttg ttttttctga tgccggttag cagtaaacta
     1681 tccactccac attcataaaa gcaaacgcgt ctgttttcat cagggcgtgc ccctttcggg
     1741 ccgggctatc cgccttactt cgtgccggct tctatccctc acctttactt tgcatttg
//
LOCUS       DAOQQY010000411         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_40579_length_1795_cov_4.889080, whole genome shotgun sequence.
ACCESSION   DAOQQY010000411 DAOQQY010000000
VERSION     DAOQQY010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40579_length_1795_cov_4.889080"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PLE23_13005"
     CDS             complement(<1..311)
                     /locus_tag="PLE23_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84232.1"
                     /translation="MNKRDFLKNSVLAGTGIFATAMGTGHARSFLADDVPYTPALGGY
                     HLDPLPYAYNALEPYIDEKTMKLHHDIHHQGYVTGLNKATDAVAAAIAANDYSLIKHW
                     ER"
     gene            complement(326..>1795)
                     /locus_tag="PLE23_13010"
     CDS             complement(326..>1795)
                     /locus_tag="PLE23_13010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX84233.1"
                     /translation="DPYKAVSSGYSQYDILNKSIIQLSDEVRLNLNVQYSRSSNIPRY
                     DRLTQIKNGKLRYAEWNYGPQIRLLVTPTLVYSPANNKLFDNLNIQPYFQKSKQERIN
                     RALNSTKRLTQTEDVTIAGLNVDINKEYAGGLTHYAGVEYILNDVKSTAVALNISNNT
                     TSPEGTRYPPGTNTMQDVGIYSKWTYKLNRMTLDGGLRYQHRSLSSNVGDDTFYPVYI
                     PDFSQSNGTLSGNLGISYAVTPHNHLKAALSRGYRAANLDDLGKIFDSSPGNVIVPSG
                     NVKPETANSAEISWVHRGPKFQFYITPFFTFLKDAIVTTPGTYNGKDSIIYQDVLSRV
                     ELITNAAEAKVYGVQAGFDLAITSNWALNYNLTYTKGRYTPIADNKEVPMDHIPPIFG
                     KLGIAYENQNLFAGFDVVFNGKKKLEDYSPTGEDNLSSATPNGMPAWIIGNLYAGYNW
                     KSLGISVGVDNILDTHYRTFASGINAPGRNFKVSLGYKF"
BASE COUNT          457 a          353 c          406 g          579 t
ORIGIN      
        1 cgttcccaat gctttatgag tgaataatca ttggcagcaa tagcagccgc tacagcatct
       61 gtagctttgt tgagcccggt tacataaccc tgatggtgaa tatcatgatg gagtttcatg
      121 gttttttcat caatataagg ctcgagggca ttgtacgcat agggcagcgg atcgagatga
      181 tagccaccta atgccggggt ataaggcaca tcatcagcaa gaaaacttcg ggcatgtccg
      241 gtacccattg cggtagcaaa tatgccggtg cctgcaagga ccgaattttt cagaaaatct
      301 cttttattca taatttaaag ttttattaaa atttgtagcc gagcgatact ttgaagttac
      361 gtcccggagc attgatacct gaagcaaatg tcctgtaatg cgtgtcaaga atattatcta
      421 ctcctacact tatgccaaga cttttccagt tatatcctgc atacagattg cctattatcc
      481 aagctggcat tccgttgggt gtagcactgc ttaggttgtc ttcacctgtc gggctataat
      541 cttccagttt cttttttccg ttaaatacaa catcaaatcc ggcaaaaagg ttttgatttt
      601 cgtaagcaat tccaagtttg ccgaatattg gcggaatatg atccatcggt acttctttat
      661 tgtccgctat gggtgtataa cggcctttag tgtaggtcaa gttgtagttg agtgcccaat
      721 ttgatgtaat ggcaagatca aatccggcct gtacgccata tacctttgct tcagcagcat
      781 tggttattag ttctacacga cttagtacat cctgataaat gatagagtcc ttaccgttat
      841 aggtgcctgg tgtggtgacg atggcatctt ttaagaaggt aaagaaggga gtgatgtaaa
      901 actgaaattt tggacctctg tgtacccaag atatttcagc actgttggct gtttccggtt
      961 tcacatttcc cgaagggaca ataacattac ccggcgatga atcaaatatt ttgccaaggt
     1021 cgtccagatt ggctgctctg tatcctcttg ataatgcagc cttaaggtgg ttgtgtggtg
     1081 tcacagcata cgagatacca agattgcctg acaaggtgcc attgctctgg ctgaaatcag
     1141 gaatgtaaac aggatagaat gtgtcatctc ctacattgct tgataatgaa cggtgctggt
     1201 atcgcaatcc tccatcaagg gtcattctgt tgagtttgta tgtccatttg ctgtagatgc
     1261 ccacatcttg catcgtattg gtgcctggtg gataacgtgt accttccggg cttgtggtgt
     1321 tgttgctaat attgagtgcc acagcagtac ttttaacatc attcaggatg tattctacac
     1381 ctgcgtaatg agtaaggcca ccggcatatt ccttattaat atcaacatta agtccggcaa
     1441 tagttacatc ttcagtctgt gtgagtcgtt tggtgctgtt caatgctctg ttgatgcgtt
     1501 cttgttttga cttctgaaaa taaggctgga tgttcagatt atcgaataat ttgttgttgg
     1561 caggcgaata tacaagtgtc ggagtaacaa gtaaccttat ctgtggacca taattccatt
     1621 ctgcatatcg gagcttgccg ttctttatct gtgtaagtct gtcataacga ggaatattgc
     1681 tgcttcgcga atattggaca ttgaggttta atcttacttc gtctgatagt tgaatgatag
     1741 atttgttgag aatgtcgtat tgcgaatatc cgcttgatac tgccttgtaa ggatc
//
LOCUS       DAOQQY010000412         1794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_40641_length_1794_cov_3.872340, whole genome shotgun sequence.
ACCESSION   DAOQQY010000412 DAOQQY010000000
VERSION     DAOQQY010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40641_length_1794_cov_3.872340"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1738)
                     /locus_tag="PLE23_13015"
     CDS             complement(<1..1738)
                     /locus_tag="PLE23_13015"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMX84234.1"
                     /translation="MEDEDGSFLITGIPIGKHSLKTTYFSYKQHEIKDIDVRQGDTTF
                     LELSLEPTAEELQEGQVVAKANRETEQTLLLDRRNSLISTQHIGAQELSGKGLGDAGA
                     AVATMTGVSKQEGVKNVFVRGLGDRYNVTLLNGFQVPSEDPEYKNIALDFFESDVIKN
                     ISVDKVFRIESVSDVGGAVVDIRSKESSETELINIEVSGKLNTNVADKTILYPDGTDY
                     FGFSKINDSGKGNFQFKNKLDPRAARIPLAGNYKISAGNRWTLNGNPFSLFAVAAHSS
                     DIEYTEEIIRNMTTDGTVYQNQTGKKHASTQRQLILANAMYETDKSTSIAYNLMLIHA
                     TNQYFGEYSGRHSEIFQGAWDETGYLRRQQINDNRLMVHQLLGEWRLAEKWRLHAGVS
                     FNNIKSAEPDRRENYLGLNQDGSYSIIGGNRQKRFFSNLNGNDFNTRVLFDYHFNKNI
                     SDNQAKISFGYSNQFGQSQFESKEYNFTSIPTKLWPDKLWLDKLYNAENYENKKFYMT
                     EGFPGSYQVTKYYHSVFATASVKISGKLSGKFGYKYDFVNSGVEYDVPGRQGNDNIHK
                     GYSLPGISLKYDI"
BASE COUNT          466 a          397 c          324 g          607 t
ORIGIN      
        1 cgatatcgta cttcaagcta atgccaggca atgaatatcc tttatgaata ttgtcattac
       61 cctgtctgcc gggcacatca tactcaactc cactatttac gaaatcgtat ttgtatccaa
      121 atttgccact aagtttcccg gatatcttga cgcttgctgt tgcaaataca gagtggtagt
      181 attttgtgac ctgatagctg ccaggaaaac cctcagtcat gtaaaatttt ttgttttcat
      241 aattttcagc attataaagc ttgtctaacc atagcttatc tggccaaagt tttgtcggaa
      301 tacttgtaaa attgtattct tttgactcaa actgagattg tccgaattgg tttgaatacc
      361 caaatgatat tttggcttga ttgtcgctta tgtttttgtt aaaatggtag tcgaacaaga
      421 ccctcgtatt aaaatcattg ccattcaaat tcgaaaaaaa acgcttctgc ctgttgccac
      481 caatgatgct gtaactaccg tcctggttta gccccaggta gttttctctc cgatcaggtt
      541 ccgcactctt aatattgttg aatgatacac ctgcgtgcaa tctccacttt tccgccaatc
      601 gccactcgcc aagtagctga tgtaccatta gtctgttgtc atttatttgt tgtcgtctca
      661 gatatccggt ttcgtcccat gcaccttgaa atatttctga atgccgacct gaatattctc
      721 caaaatattg gttagtggca tgtatcagca tcagattgta agcaatactg gttgatttat
      781 ctgtttcata cattgcattt gccagtatga gctgtcgctg ggtagaagca tgtttcttac
      841 cggtctgatt ttggtaaact gtaccatctg tagtcatatt cctgatgatc tcttcagtgt
      901 attcaatatc tgaggagtgt gctgccacag caaacagtga aaaaggattg ccattcaaag
      961 tccaacggtt accggcagat attttgtaat taccggccaa cggtattctg gcagctcttg
     1021 ggtcgagctt gttcttgaat tgaaaatttc ctttcccact atcattgatt tttgaaaatc
     1081 cgaaataatc cgttccatca gggtacagga ttgttttgtc tgctacattc gtgttaagtt
     1141 ttcctgatac ttctatattg atcagctcag tttcagacga ttctttggag cgtatatcta
     1201 cgactgcacc accgacatca cttacactct ctatacgaaa aactttatcc acagaaatgt
     1261 tctttatcac atccgactca aagaaatcaa gagcgatatt cttgtattcg ggatcttcgg
     1321 aaggtacctg gaagccattg agcagcgtga cattataccg atcgcctaat cctctgacga
     1381 acacattttt gacaccttct tgctttgaca cgccggtcat agtggcaacc gctgcaccag
     1441 catcgcctaa gccttttcct gacaattcct gggctccgat atgctgagta gatatcagtg
     1501 aatttctgcg atcaagaagt aatgtctgtt ctgtttcacg atttgcttta gctactacct
     1561 gcccttcctg caattcttca gccgtgggtt cgaggctcag ctctaagaat gtagtatcac
     1621 cctgtcttac atcgatatcc tttatttcgt gttgtttata agaaaaatac gttgttttaa
     1681 ggctgtgctt gcctatcggt atgccggtga ttaaaaaact accatcttca tcttccaaaa
     1741 ttaactccca tttttgaata acaattatag ctaatttaaa tttcctatct ataa
//
LOCUS       DAOQQY010000413         1737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_43131_length_1737_cov_4.382283, whole genome shotgun sequence.
ACCESSION   DAOQQY010000413 DAOQQY010000000
VERSION     DAOQQY010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43131_length_1737_cov_4.382283"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(171..626)
                     /locus_tag="PLE23_13020"
     CDS             complement(171..626)
                     /locus_tag="PLE23_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84235.1"
                     /translation="MKHILFLFFLVAGTFSLHAQNDRFPLVANSNCNNIDGKPWRSCG
                     LATLYSTGKFLCSVKTESANPQGFSGAVLFTFYSEDGKPVLTIETPAYTVKGKASTLV
                     PFSEMLDKKLVQHIFRFEAKGIRSSNTKTIKKLLPEGEKLMKAAATAKK"
     gene            910..>1737
                     /locus_tag="PLE23_13025"
     CDS             910..>1737
                     /locus_tag="PLE23_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMX84236.1"
                     /translation="MKIEYSHMLYDQNPTLDKLQDKTHDESIQGQAYGQEEEIDTGKK
                     KFYIESYGCQMNFSDSEIVASILSGMGFVPTQQIARADLILVNTCSVRDKAEETVRKR
                     LRVFNAEKTKRPGTMIGVLGCMAERLKKKFLEEERLVDMVVGPDSYRDIPNLIATVEE
                     GDKGINVLLSREETYADIAPMRLDSNGICAFISIMRGCDNMCSFCVVPFTRGRERSRD
                     AFTIISEAQDLYNRGYREVTLLGQNVDSYKWENPETSHIVDFAQLLKMVAEVNPLLRV
                     "
BASE COUNT          527 a          311 c          405 g          494 t
ORIGIN      
        1 atggcaataa taagcaatgt aatggacata cgtatgtata gataagtgat gcaaacttac
       61 cgtacaatgg aacaaaaaca aaacatggca gatttattta ttgaatccgg gctgtgctgc
      121 ctgtcgtaaa ccatatattc aacataagtt gccggaagtg tttgtaatgt ttactttttg
      181 gcagtggcag cagctttcat cagtttttcg ccttccggaa gcagtttctt aattgttttg
      241 gtattggaag aacggatacc tttggcttcg aatctgaata tgtgctgtac gagctttttg
      301 tctaacattt cactgaatgg taccagtgtt gatgctttgc ctttgacagt ataagccggt
      361 gtttcgatgg ttaaaaccgg tttgccgtct tctgaataaa atgtaaacaa aactgcacct
      421 gaaaatcctt gtggattagc agattcggtt tttactgaac aaaggaattt gccggtggaa
      481 tatagcgttg caagtccgca tgagcgccag ggtttaccat cgatgttgtt gcaattagaa
      541 tttgccacaa gtggaaatct gtcgttttga gcatgcaatg aaaaagtacc tgctactaaa
      601 aagaaaagga ataatatatg tttcatgact atgatttttg taaaacaaaa ctacccggaa
      661 agaccaaagc aaatcaatct ttaccggctt cgacttgcaa tcaaacgtat attttcaaaa
      721 atagtgactt cgaaacaggt attttaacat aacgacaata gtacggaagt ttgccatctt
      781 cagttaagtg tagattttta atagtatggt agtatggaca aattgattat taacaaatat
      841 ttacgatatc atcgaatgac attttgtcac ctaataaatg taattttcgt atatttgcat
      901 tctgtttaaa tgaaaatcga atattcgcac atgttatacg atcaaaatcc tactttagat
      961 aaacttcagg acaagacaca cgatgaaagc atccagggtc aggcttatgg ccaggaagaa
     1021 gaaattgata cgggcaaaaa gaaattttac atagagagct acgggtgcca gatgaatttt
     1081 agcgatagcg agattgttgc ttctatccta tcaggaatgg gcttcgttcc gacacagcag
     1141 atagcaaggg ctgatttgat attggtgaat acttgttcgg tacgtgataa ggccgaagaa
     1201 acggtaagga aaaggctgag agtgttcaat gctgaaaaaa ccaagaggcc aggcaccatg
     1261 ataggtgtac ttgggtgtat ggccgaacga ctcaagaaaa aattcctgga agaagagcgg
     1321 ctggtggaca tggtggtagg ccccgattcg taccgcgata ttccgaacct gatagctact
     1381 gttgaagaag gcgacaaagg cataaacgtg ttgctctcaa gggaggagac ttatgccgac
     1441 atagcaccga tgcgtttgga ctccaacggt atctgtgcct ttatatccat catgcgaggc
     1501 tgtgataata tgtgttcgtt ctgtgtcgta ccgtttacgc gtggtcgaga gcgttcgcgt
     1561 gatgcattca ccattatctc agaggcacag gacctgtaca accgcggcta ccgtgaggtg
     1621 accctgcttg ggcagaatgt agattcgtac aaatgggaaa atcctgagac aagccatata
     1681 gttgactttg cccagctatt gaagatggtg gcagaggtga atcctttgct tcgggta
//
LOCUS       DAOQQY010000414         1715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_44081_length_1715_cov_5.740964, whole genome shotgun sequence.
ACCESSION   DAOQQY010000414 DAOQQY010000000
VERSION     DAOQQY010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44081_length_1715_cov_5.740964"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLE23_13030"
     CDS             complement(<1..465)
                     /locus_tag="PLE23_13030"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HMX84237.1"
                     /translation="MDHLTNKTIAIHQPNYLPWPGYFYKISRCDEFVFLDHVQINKRG
                     LTRRTQLPDRNHPGQTIFLTVPLRKFSDSTPINALFIDHTKNWQTQHLNKIRDYYQRF
                     PYFEAYYNLFEKWLNYSIQYEKLSELNIYLIKEICTLLGIRSRWHLSSNMPIT"
     gene            complement(453..1097)
                     /locus_tag="PLE23_13035"
     CDS             complement(453..1097)
                     /locus_tag="PLE23_13035"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HMX84238.1"
                     /translation="MNIFNPANKRILVLAPHTDDGELGCGASIVRFMEEGADVYYAAF
                     STADESVPSEFPKDQLAIEVKEATKILGIADNHLFVFQYQVRKLNYVRQEILEKLIEL
                     REDLKPDIVFLPSSADFHQDHQTVTQEGLRAFKFSTILGYELIWNNRSFSTDCFIKIK
                     EEHLHRKITALSAYRTQAKKTYMSPEFIRSLAVVRGTQIGVAFAETFEVIRWII"
     gene            complement(1072..>1715)
                     /locus_tag="PLE23_13040"
     CDS             complement(1072..>1715)
                     /locus_tag="PLE23_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HMX84239.1"
                     /translation="NTGVQNLLKAGITEHVYHTGDVGMDLLFNLGQEFEASKDLLDRM
                     GISAGNYIFATCHRAANTDNIENLTEILRAFAAAPRPVVFPVHPRTYKVMVQYNLLDA
                     IPQCKLILTDPFGFTDTQILIKNAAMVLTDSGGVIKEAYFHQVPSIIIDKQTEWLEIL
                     EEGWSVIAGPSFENISRYLHNFEIPEHHGMSLGDGKASEKIAQLIYEYLQSGQ"
BASE COUNT          439 a          339 c          396 g          541 t
ORIGIN      
        1 ggtaatcggc atattagaag acagatgcca tctagacctg ataccgagta atgtgcagat
       61 ttcctttatc aggtaaatgt tgagctcgct gagtttttcg tactgaattg aatagttgag
      121 ccatttttcg aacaaattgt aataggcttc aaagtagggg aatctttggt aatagtctct
      181 aattttgtta agatgttgag tttgccaatt ctttgtatgg tcgataaata gcgcattgat
      241 aggtgtactg tctgaaaatt ttcgaagagg aacagtcaga aaaattgttt gtccgggatg
      301 attcctgtcg ggcaattgtg tacggcgggt caatcctctt ttgttgattt gcacgtggtc
      361 aaggaacaca aactcatcac atctgctgat tttatagaaa taccccggcc agggcaagta
      421 gttgggctga tgaatggcta tagttttgtt tgtcagatga tccatcggat gacttcgaat
      481 gtttctgcaa aggctacacc tatctgtgtg cccctgacta cagcaagcga tcggataaat
      541 tcgggcgaca tataggtttt ttttgcctgc gtcctgtaag cggataaagc agttatcttg
      601 cgatgtaggt gctcttcttt gatttttata aaacaatcgg tactgaatga tctgttgttc
      661 cagatgagtt cgtaacccag aatagtactg aacttgaatg cccgaagacc ttcctgggtg
      721 acagtctgat ggtcctggtg aaaatcggcc gaagaaggta gaaatacaat atccggtttg
      781 agatcttccc ggagttcgat gagtttttcc agtatttctt gtcttacgta attcagtttt
      841 ctaacttgat attggaatac aaacagatga ttatctgcta tgcccagtat tttagtagcc
      901 tccttcactt cgattgccag ctggtctttt gggaattcag aggggacaga ctcgtcagct
      961 gtagaaaatg ctgcataata gacatcggct ccttcttcca taaacctgac tatagatgcg
     1021 ccgcagccaa gctcgccgtc atccgtatgt ggtgccaaca ccagtattct tttattggcc
     1081 ggattgaaga tattcataga tcagttgtgc gattttttct gaggcttttc cgtcaccaag
     1141 gctcatgccg tggtgttccg gaatttcaaa attatgcaaa tatcttgaaa tattttcaaa
     1201 agatggtcct gcaatcactg accagccttc ttctagtatt tcaagccatt cggtttgctt
     1261 gtcgatgatg atggaaggca cttggtgaaa atatgcctct ttgatgacac caccgctgtc
     1321 tgtgagtacc attgctgcgt tttttatcag tatttgggta tctgtaaagc caaaaggatc
     1381 tgtaagtatt agcttgcatt gtggaatagc atccaacaga ttgtactgta ccattacctt
     1441 ataagtacga ggatgcacag gaaacactac cggccggggt gctgctgcaa atgctcggag
     1501 tatctcagtg aggttttcga tattgtctgt attggcagct cggtggcaag tggcgaatat
     1561 gtaattgccg gccgaaatac ccattcggtc aagcaaatct ttacttgctt caaattcttg
     1621 acctaagttg aacaagagat ccatgccgac atcacctgtg tggtacacat gctctgtgat
     1681 accggccttt agtagattct gcacgcctgt attgg
//
LOCUS       DAOQQY010000415         1708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_44425_length_1708_cov_4.194797, whole genome shotgun sequence.
ACCESSION   DAOQQY010000415 DAOQQY010000000
VERSION     DAOQQY010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44425_length_1708_cov_4.194797"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /locus_tag="PLE23_13045"
     CDS             <1..1397
                     /locus_tag="PLE23_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84240.1"
                     /translation="SAIFTLTLAWGGNFFLNEFFFDYLPMFNKFRAVSMALGLSQFFA
                     IILGVWGLQELMNNNIPREKKLKSLYLAAGITGGVVILGLLMSSGMAFVGKNDDKVGP
                     DLAALLKQDRISILRADAMRSFLLIVVAAGLIWAYLKHKLKSVVAISAVAILAVMDIV
                     LVNKRILFDEKFENPSTELQAGPAPTEVDKQVMADKDIDFRVLDLRSGNPFTDAHTSY
                     FHKSLGGYHAAKLMRYQELIERYLSQPAEHMNILNMLNTKYILQRNDAGETAVPNPGA
                     LGNAWFVRDFKTVENADAEMDALKGLDAGKMALIRKSDENKLSGWKKEADTTATIKLA
                     SYHPDKMVYEYHSQSPQLAVFSEIYYPPAKGWKVTLDGKPFDDFFKVDYLLRGLVVPA
                     GNHKIEMNFHPDSFYKGETISKVFSGLILVLFVLGMVIYFKKEGLPDVTHMEELPEEE
                     KAKKAIPVTKAKKK"
BASE COUNT          488 a          348 c          374 g          498 t
ORIGIN      
        1 tgagtgctat ttttacgctc acgcttgcct ggggaggcaa ttttttccta aatgaatttt
       61 tcttcgacta cctgccaatg tttaataaat tccgagctgt ctctatggca cttggtttgt
      121 cccagttttt tgccatcata ctcggcgtat ggggtcttca agaattgatg aacaacaata
      181 ttccgagaga gaaaaaactc aaatcgctct acctggcagc gggtattacc ggcggtgtgg
      241 tcatactcgg cctactgatg agctcaggta tggcatttgt aggtaaaaat gatgataagg
      301 ttggcccgga tctggcagca ttgctcaagc aagacagaat atcaatactt cgtgcagatg
      361 ctatgcggtc atttttgctt atcgttgtgg cagcaggatt aatctgggct tatctgaagc
      421 ataagttaaa atcggttgtc gccatttcgg ctgttgcaat attggcggta atggatatcg
      481 tgttggtcaa taaacgtatc ttgtttgatg aaaaattcga aaacccttct actgagctcc
      541 aggccggacc tgcacctact gaagtagata agcaagtaat ggcagataag gatatcgact
      601 tcagggttct cgacctgcgc agtggcaatc cctttaccga tgcgcatact tcctatttcc
      661 ataaatcgct gggtggctat catgccgcta agcttatgcg ttaccaagaa ctaatcgaac
      721 gctatctgag ccagccggcc gaacacatga acatattgaa tatgctcaac acaaagtata
      781 ttctccaaag gaatgatgcg ggagaaactg ccgtgcctaa tccgggtgca ctcggcaatg
      841 cgtggttcgt acgtgacttc aaaacagtgg aaaatgcaga tgcagaaatg gatgcactca
      901 aaggtcttga tgccggtaaa atggccctta tccgaaaatc agacgaaaac aaactgtccg
      961 gctggaaaaa ggaagcggac actaccgcta ctatcaaact cgcgtcatac catcctgata
     1021 aaatggtgta tgagtaccat tcgcagtcac cacagttggc agtattttct gaaatatact
     1081 acccacctgc taagggttgg aaggtaacgc ttgatggaaa accgttcgat gatttcttta
     1141 aggtcgatta tctgttacgt ggtcttgtgg tacctgccgg caatcataag attgaaatga
     1201 atttccatcc tgactccttc tataaaggcg aaacaatcag taaggtattc tcaggactga
     1261 tccttgtctt gtttgtactt ggtatggtta tttattttaa gaaagaagga ttgccggacg
     1321 tgacacacat ggaggaatta cctgaagaag agaaagctaa aaaggccatt ccggttacaa
     1381 aagctaaaaa gaaataaaga aaattctttt acattgccaa aagccgatga gtattaaact
     1441 tttcggcttt ttattttgat tcaaccttat tggatttaaa cgacattgat tagtacaata
     1501 aatccataac gaagtgataa aatcatattg tacttttggt ttttaagcat ttgcaatgga
     1561 ttggaactaa tcattatttg atccaaaaaa tatcatgcgt tatccgggtt ttatcatggc
     1621 gtgcccctgt cgggtcgggc tatacgcctt acgttgtgcc ggcttctatc cctcaccttt
     1681 actttgcatt tgagattaaa ttcttaac
//
LOCUS       DAOQQY010000416         1704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_44600_length_1704_cov_6.128563, whole genome shotgun sequence.
ACCESSION   DAOQQY010000416 DAOQQY010000000
VERSION     DAOQQY010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44600_length_1704_cov_6.128563"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1704
                     /locus_tag="PLE23_13050"
     CDS             <1..>1704
                     /locus_tag="PLE23_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84241.1"
                     /translation="IYWSNATARVSESTKDTLLPVWLQNIEQASGAHKAVLQSVLAEM
                     IWNYLEGNRWLIHGRTTTEAFEKSDMATWSEKDFEMIAAELYLESVSHPEFLKIQVGK
                     YSDLLTPGQNSDTRRPFLFDVLVARAVEHFTNTRNNLHKPVNPFRLNDDALFLPANQF
                     VRYEFATHDTISPDYRIVSLLQQWLLFHETDTTKDAWLDADLARLEFVNNNSTLKNKS
                     AFYRERLSQLIEQHKDSRLIANFYARLAMSYMTSAAEWDGTSELKKWDYKTAFDLATE
                     CVNKYGKLSGTDECKNILNTLTQLRLNVTNEEIYTAGKPLLVLLNYNQSKHLYYSVIK
                     TNNEIDEVLKKMEQYGNRDSIVQYLSKLKVLESGHYDLPNRGDFRENSVEFMVPSLDF
                     GSYILLFGSNSQFTNNHSNAAYYSRFDVSNLTITTGNSSPTTDIMVTDRNTGQPLKDV
                     IIRITELKSEYVKNEWVEKSTILYDNIKTDKDGFVHSKIALKAGEYNSSILIEAYSKK
                     DYAKVRKSITNYREPGVSESFIKTNFYTDRAIYRPGQTIYFKGLVLSVMDGRSNVLAG
                     K"
BASE COUNT          563 a          331 c          351 g          459 t
ORIGIN      
        1 tgatttactg gtcaaatgcc acagcaagag tatctgagtc aacaaaagat acactactgc
       61 ctgtctggtt gcaaaacatc gagcaggcaa gcggtgcaca taaggctgta cttcagtcag
      121 tattggcaga gatgatctgg aattatctcg agggtaatcg ttggctcatc cacgggcgta
      181 ccactacgga agcatttgaa aagagcgaca tggctacctg gtcagagaaa gattttgaaa
      241 tgatagctgc agaactatat cttgagtctg taagccatcc tgaatttttg aagattcaag
      301 ttggaaaata ttctgatcta ttgacacctg gtcaaaattc ggatacacga agacctttcc
      361 tgtttgatgt acttgtggca agggcagttg aacattttac gaatacccgg aataacctac
      421 acaagcctgt taacccattc agactgaatg atgatgccct ctttttaccg gctaaccagt
      481 ttgtgcgata cgagttcgcg acacacgata caataagccc cgattatagg attgtcagcc
      541 tactccagca atggttgtta tttcacgaaa cggatacaac taaagatgca tggttggatg
      601 ctgacctggc cagactcgaa tttgtcaaca acaacagcac cttaaaaaat aaatctgctt
      661 tttacagaga gcgtctcagc caattaattg agcaacataa agattcaagg ctgatagcca
      721 atttctatgc acgtcttgca atgagttata tgacttcagc agcagaatgg gacggtacat
      781 cggaactcaa gaaatgggat tacaaaactg cattcgatct tgctacagaa tgtgtcaaca
      841 aatatggcaa attgtcaggc acggatgaat gcaagaatat cttaaatacg ctgacacaac
      901 tcagacttaa tgtcaccaac gaggagattt atactgccgg aaagccactt cttgttttac
      961 ttaattataa tcaatccaag catctgtatt acagcgtgat aaaaacgaat aatgagattg
     1021 atgaagtatt gaaaaaaatg gaacaatacg gaaaccgtga cagcattgtt cagtatttga
     1081 gtaagttaaa ggtactagag tctggtcatt atgatcttcc caaccgcggc gatttccgcg
     1141 aaaattcagt cgaatttatg gttccttcgc ttgatttcgg atcgtatatc ctgctgtttg
     1201 gttctaattc ccaattcact aacaaccatt caaatgctgc ttattactcc aggtttgatg
     1261 tgagtaatct tactatcacg accggcaatt cgtcacctac cactgatata atggtcaccg
     1321 accgtaatac cggacagcca ttgaaagatg taataataag gataacagag ctaaaaagcg
     1381 aatatgtgaa aaatgagtgg gttgagaaaa gcactatact atacgataac attaaaacgg
     1441 acaaagatgg ttttgtgcat agcaagattg cgttgaaagc aggtgaatac aacagcagta
     1501 ttctgataga agcatactca aagaaagatt atgcaaaagt tagaaaatca ataacaaact
     1561 acagagaacc aggagtttct gagtctttta tcaaaacaaa cttttatacc gacagagcta
     1621 tttatcgccc ggggcaaact atttatttca aaggtcttgt gttaagtgtc atggatggcc
     1681 gttctaatgt actagctggg aaaa
//
LOCUS       DAOQQY010000417         1671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_46236_length_1671_cov_4.735149, whole genome shotgun sequence.
ACCESSION   DAOQQY010000417 DAOQQY010000000
VERSION     DAOQQY010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46236_length_1671_cov_4.735149"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PLE23_13055"
     CDS             complement(<1..383)
                     /locus_tag="PLE23_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMX84242.1"
                     /translation="MSTINKTTENGICYIYLNRPEVYNSFNREMALELQHALDDAEEN
                     NDVRCIVISGTGKAFSAGQDLGEVTGPNPPGFNTILGEHYNPIILRIRNIEKPVIAAV
                     NGVAAGAGANIALCCDIVVAAQSAS"
     gene            complement(439..939)
                     /gene="paaJ"
                     /locus_tag="PLE23_13060"
     CDS             complement(439..939)
                     /gene="paaJ"
                     /locus_tag="PLE23_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HMX84243.1"
                     /translation="MMNTTTVTKEQIFEILDQVPDPEVPVLSTLDLGIIREVLIGDDG
                     VEIIITPTYTGCPAMNMFEVNIIAALQVNGIPNVKVTTVLSPAWTTDWLSEKGKEKLR
                     AYGIAPPVGKTTDKHVIMGEEKHVKCPQCGSADTEMISHFGSTACKSLYKCLECKEPF
                     EYFKCH"
     gene            complement(942..>1671)
                     /gene="paaC"
                     /locus_tag="PLE23_13065"
     CDS             complement(942..>1671)
                     /gene="paaC"
                     /locus_tag="PLE23_13065"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HMX84244.1"
                     /translation="TLRLADNASILGHRLGEWCGHGPVLEQDIAMTNIALDLIGQARN
                     YYQYAAKLEAAGKTEDDYAYLRDPLDYYNLLLLEQPNGNFAHSIVRQYFFDIFHYLFL
                     TALMSSKDKQLAAIAEKSIKEVTYHKKWSGDWILRLGDGTELSNEKMQTAINVLQTYV
                     GELFLMDEVDEMMVKENIGVDLAPIKEEWTRIVQEHFTEAKLKLPDATWTQKGGKQGY
                     HSEHLGYILADMQYLQRTYPGAVW"
BASE COUNT          398 a          367 c          399 g          507 t
ORIGIN      
        1 aatgaagcac tttgggcagc tactactatg tcgcaacaca gggcaatatt ggcacctgca
       61 ccggctgcca caccgttaac tgctgcgatg acgggctttt caatattcct gatacgtaaa
      121 atgatgggat tgtaatgttc gccaaggatg gtattgaatc ctggcggatt agggcctgtc
      181 acttcgccaa ggtcctgacc tgctgagaaa gctttgcctg tgcctgagat aacgatacac
      241 ctgacgtcat tgttctcttc tgcatcatcg agggcatgtt ggagttcaag cgccatttct
      301 cggttgaagc tgttatatac ttctggtctg ttgagataaa tgtagcagat gccgttttcg
      361 gtagtcttat tgatggttga catgtttttt ttgtgtaaaa ttagcaaatt tcacgtaaaa
      421 catcagcctg cataagcttc aatgacattt aaaatactcg aaaggctcct tgcattcaag
      481 acatttgtac aacgacttgc atgcagtaga gccaaagtgg gaaatcattt ctgtatctgc
      541 agaaccacat tgtgggcatt tgacatgctt ttcttcaccc atgattacgt gtttgtcggt
      601 tgttttgcca acaggcggtg cgataccgta agcgcggagt ttttccttgc ctttttcgga
      661 gagccagtcg gtggtccagg caggtgacag aacggtagtg actttcacat tagggatgcc
      721 gttgacttgt agagcggcaa tgatgttaac ctcgaacatg ttcatggcag gacagccggt
      781 gtaggtaggt gttataatga tttccacacc atcatcaccg atgagcactt cgcggatgat
      841 gcccaagtca agtgttgaca atacgggaac ctccgggtca ggtacctgat caaggatttc
      901 gaaaatctgc tcttttgtta cagtcgtcgt attcatcatt tttaccaaac tgctcctgga
      961 tacgtacgct gcaggtattg catatcggcc aggatgtaac cgagatgttc gctatggtag
     1021 ccctgttttc cgcctttttg tgtccaggtg gcatccggta gttttagttt tgcttcggtg
     1081 aaatgttctt gaacgattct ggtccattct tcttttattg gtgccagatc tactccgata
     1141 ttttctttca ccatcatttc atccacttca tccatcagga acagttcgcc cacataagtc
     1201 tggagtacat tgatggcagt ctgcattttt tcattgctca gttcggtgcc atcgccgagc
     1261 cgtagaatcc agtcaccgct ccattttttg tggtaagtca cttccttgat cgatttttcg
     1321 gcaatggctg ccagttgttt gtcttttgaa gacataagcg cagtaaggaa aaggtaatgg
     1381 aatatatcga aaaaatactg ccttacgatg ctatgggcaa aattgccgtt aggctgctca
     1441 agtaataaca ggttgtaata gtcgagcgga tcgcgcaggt aagcgtagtc atcttccgtt
     1501 ttaccggcag cttcaagttt tgctgcatat tgataataat tgcgtgcttg ccctatgagg
     1561 tcgagagcga tgttggtcat agcaatatcc tgctcaagta caggtccatg gccacaccat
     1621 tcgccaaggc ggtgacccaa gatggatgca ttgtctgcaa gccgaagtgt a
//
LOCUS       DAOQQY010000418         1592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_50679_length_1592_cov_3.043591, whole genome shotgun sequence.
ACCESSION   DAOQQY010000418 DAOQQY010000000
VERSION     DAOQQY010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50679_length_1592_cov_3.043591"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..690)
                     /locus_tag="PLE23_13070"
     CDS             complement(112..690)
                     /locus_tag="PLE23_13070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMX84245.1"
                     /translation="MSFKDTKLHENPWQILSTNTKYENNWLRVDHHDVVNPAGNPGIY
                     GTVHFKNLAVGVVVLDDENNTYLVGQYRFPLGQYSWEIPEGGAPLDQDPLEMAMKELK
                     EETGLTARNWIKLAKLHTSNSATDEAAIIYLASGIETGIAEPEETEILNVKKIPFTKY
                     VEMIEDGEITDAISVCAGLLTYRWLNKQNQKT"
     gene            711..1172
                     /gene="coaD"
                     /locus_tag="PLE23_13075"
     CDS             711..1172
                     /gene="coaD"
                     /locus_tag="PLE23_13075"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMX84246.1"
                     /translation="MKIAVFPGSFDPITVGHVDLVRRALPLFDKIIIAIGVNSQKKYL
                     FSLEQRKNWLKDVFNDLGAVEIDVYESLTAKYCEEKNARFILRGLRNAADFDYEKTIS
                     QLNHIIGNGVETIFLNSQPQYSHISSTIVREIILGKGDASPFLPAQVKITM"
     gene            1197..>1592
                     /locus_tag="PLE23_13080"
     CDS             1197..>1592
                     /locus_tag="PLE23_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013767014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMX84247.1"
                     /translation="MPNSIVKVPKAVNEPVLNYAPGSPEKFELKSTLKSFKETSFDVA
                     MTIGGKKVTTDNKVAIKPPHELSHTLGYFHKGDKTHVDMAITAALAAKADWEEMPWEH
                     RAAIFLRAADLLAGPYRARMNAATMLCQSK"
BASE COUNT          494 a          313 c          300 g          485 t
ORIGIN      
        1 aaatactggc tgattgaaga ggattgcctt cattgtcagt gaccgtaccg tataattgtg
       61 ccgatacaca ttttacaaaa aatagtataa aaaagacact caatatccac ttcatgtttt
      121 ttggttttgt ttgttaagcc atctataggt cagcaaccct gcacatacac taatggcatc
      181 agttatttcg ccatcttcta tcatctctac atatttagta aagggaatct ttttaacatt
      241 cagaatttcc gtttcttcag gttcggcaat acctgtttct atgcctgaag ctagatatat
      301 aatagcggct tcgtcggtag cagagttgga tgtatgcaat ttggcaagtt ttatccaatt
      361 ccttgcagta agtcctgttt cttctttcag ctctttcatg gccatctcca gagggtcttg
      421 gtctaatggt gcaccgcctt cgggtatctc ccatgagtat tgcccaagtg gaaacctata
      481 ttggcctact aaatatgtat tattttcatc gtccagaaca acaacaccaa cagctaagtt
      541 tttgaaatga actgtaccgt aaataccggg attaccggcc ggattaacaa catcgtgatg
      601 atcgacacgc agccagttat tctcatattt tgtgttagtt gacaatatct gccatgggtt
      661 ctcatgtaat tttgtatctt tgaaggacat aatatataat gattcattgt atgaaaatag
      721 cggtttttcc aggttcgttt gatccaatta ctgtaggtca cgttgatttg gttcgaagag
      781 ctcttccatt attcgacaaa ataatcatag caatcggagt taattcacaa aagaaatacc
      841 tttttagttt agaacaaaga aaaaactggc ttaaagatgt atttaatgat ttgggtgcgg
      901 ttgaaatcga cgtatatgaa agtcttacag ctaaatattg tgaggaaaag aatgcaagat
      961 ttatccttcg aggtctgaga aatgctgccg attttgatta cgaaaaaacc atttcacaat
     1021 taaaccatat cataggtaat ggtgttgaaa ctatatttct caacagtcag ccacagtatt
     1081 cacacattag ctcaacgata gtacgtgaaa ttatccttgg aaaaggtgat gcgagtccgt
     1141 ttttacccgc tcaagtcaaa atcacgatgt aactttgtaa aaaaatattt ttaaatatgc
     1201 caaattcaat agtaaaagtt ccaaaggctg tgaatgagcc tgtattgaac tatgcacctg
     1261 gttcgccaga aaaatttgaa cttaaatcca cactcaaatc tttcaaggaa acgagttttg
     1321 atgttgccat gaccatcggt ggtaaaaaag taacaaccga caacaaagtt gctatcaaac
     1381 ctccgcatga gctatcccac actttagggt attttcacaa aggtgataaa acacacgtag
     1441 atatggctat cacagccgca cttgcagcaa aagcggattg ggaagaaatg ccttgggagc
     1501 accgtgccgc aatatttctt agagcagccg atctgcttgc tggcccatac agagcacgta
     1561 tgaatgcagc taccatgcta tgccaatcaa ag
//
LOCUS       DAOQQY010000419         1556 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_52883_length_1556_cov_5.215190, whole genome shotgun sequence.
ACCESSION   DAOQQY010000419 DAOQQY010000000
VERSION     DAOQQY010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52883_length_1556_cov_5.215190"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..433)
                     /locus_tag="PLE23_13085"
     CDS             complement(107..433)
                     /locus_tag="PLE23_13085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84248.1"
                     /translation="MLVQEFLPVANEFELDSLDESVSEEMLFNLLSSKIAELLDAGRL
                     EYLLNLLYRHDVNENLIRQALDPASEELPHTAIATLLIERFKKKMETRLRYKTPPVSD
                     WLNFDE"
     gene            complement(437..>1556)
                     /locus_tag="PLE23_13090"
     CDS             complement(437..>1556)
                     /locus_tag="PLE23_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMX84249.1"
                     /translation="NKPESQIFAGKNLIMGGSLSEFSEYPHSQIGDVSAIMMAHMPAF
                     AKPFYAPNLEIGLMSEFEKKIELTARNALDINVTMIGGVPTWTIVLFRRILEITGKSN
                     MLEVWPNFETYIHGGVSFLPYKEQFKRFFPSDSVRYVEIYNASEGYFAIQNDFNTDDM
                     LLLLDNGIYYEFLPMEEWDTENPQTVLLEEVEAGKNYAIIISTNAGLWRYMPGDTVVF
                     TSTSPYKIKVSGRTQQFINAFGEEVMVSNTDQALAAACKEFDAEVSEYTVAPYYFEND
                     GKGRHQWLIEFEKIPVDVNAFADSLDKNLQKINSDYEAKRYLDLALTNLSIQTLPKGT
                     FHQWMKQRGKLGGQNKIPRLANNRKYVDELLHYLNHPA"
BASE COUNT          411 a          310 c          343 g          492 t
ORIGIN      
        1 aatatagccg tttgatgctg atgcatgtgc aaatatgcaa attatttatc aaacaacaga
       61 aacacttaat ccattcgttt gccgacaaga gtagcctgag acagtattat tcgtcaaaat
      121 taagccaatc acttacaggt ggcgttttgt atctaagtcg ggtttccatc ttttttttga
      181 atcgttcgat gagtagtgtt gcaattgcag tatgtggcaa ttcttcggaa gccgggtcaa
      241 gtgcttgtcg tataagattt tcgttgacat catgcctgta gagcaggttg agaagatatt
      301 caagacggcc ggcatcaaga agttcggcga ttttggaaga tagcaaatta aacagcattt
      361 cttcggagac actttcgtca agtgaatcga gttcgaattc gttagctaca ggtaaaaatt
      421 cttgaacgag catatatcag gccggatgat ttaaataatg aagtagttcg tcaacatatt
      481 ttcggttatt ggcaaggcga gggattttgt tttgacctcc gagtttgcct cgctgcttca
      541 tccattgatg gaaggtacct tttggaagag tttggatgct taagttggtc aatgccagat
      601 cgagatagcg ttttgcctcg tagtcgctat tgattttctg aaggtttttg tcaagtgaat
      661 cagcaaaagc attaacatca acaggtattt tttcaaattc tatcagccac tggtgtcggc
      721 ctttgccatc attttcaaaa taatatggag ccacagtgta ttctgatact tctgcatcaa
      781 actccttgca agcagctgcc agtgcctgat cggtattgct caccatcact tcttcgccga
      841 atgcattgat aaactgttgt gtacggccac tcactttaat cttgtatggg ctggtactgg
      901 taaacactac ggtatcgcca ggcatatagc gccacaggcc ggcattggta gagataatga
      961 tcgcataatt ttttcctgct tctacttctt cgagcaatac tgtctgtggg ttttctgtat
     1021 cccattcttc cataggcaag aattcgtagt aaatgccatt gtctagtaac agcagcatgt
     1081 catctgtatt gaaatcgttc tggatggcaa aatagccttc tgaggcattg tatatttcga
     1141 catatctgac cgaatctgaa gggaagaatc gtttgaattg ttctttataa ggcaaaaagc
     1201 tgacgccacc gtgaatgtat gtctcgaaat tgggccatac ttccagcata ttcgatttgc
     1261 cggtgatttc gaggattcgg cgaaacaata cgatggtcca ggtaggtaca ccacctatca
     1321 tggttacgtt gatatccagt gcattacgtg ctgtcagttc aatttttttc tcaaattctg
     1381 acatcagtcc gatttcgagg tttggggcgt agaacggttt ggcaaatgcc ggcatgtgtg
     1441 ccatcataat agcagaaaca tcacctatct gcgaatgtgg atactcgctg aattcagata
     1501 atgatccccc cattatcagg ttttttcctg caaaaatctg actttcgggc ttattg
//
LOCUS       DAOQQY010000420         1517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_55445_length_1517_cov_4.549932, whole genome shotgun sequence.
ACCESSION   DAOQQY010000420 DAOQQY010000000
VERSION     DAOQQY010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55445_length_1517_cov_4.549932"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLE23_13095"
     CDS             complement(<1..443)
                     /locus_tag="PLE23_13095"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMX84250.1"
                     /translation="MLRFLVLICFMVWMPFSGNAQDTLADKQVRIISKDTVQLNIPKG
                     KFQPKPTKALVYSLIIPGAGQIYNRDWWKLPLVYGALGGVGYLVQQNYKEYRRYDKAR
                     DLFLEGKPNEFSHLNPNEASLRAARDYYRSNFELSCLGLGVLYAL"
     gene            complement(453..1016)
                     /locus_tag="PLE23_13100"
     CDS             complement(453..1016)
                     /locus_tag="PLE23_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HMX84251.1"
                     /translation="MDLKQIFSVSLILFSVIDILGALPIIIKLKADGKQIEPLKVTLV
                     SGSIMIGFLFAGEGILSLLSLDVQSFALAGAIVLFLIGIEMILGIVLFKDTGMNDKPS
                     VVPLAFPLIAGAGTMTTLISIKTTFEVINIVIGIVLNLVFIFFTLRISGWLQRQLGEG
                     GTNIIRKVFGVILLAISIKLFKTNFPG"
     gene            complement(1020..>1517)
                     /locus_tag="PLE23_13105"
     CDS             complement(1020..>1517)
                     /locus_tag="PLE23_13105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMX84252.1"
                     /translation="RKDLNPLEVAFTYQRLLDECNLTHEALATRVAKDRSTVTNFLRL
                     IKLPPEIQSALKTQELSMGHARALAGIEDPVKMIFLYNQVRNKGMSVRETESLIRDYQ
                     TPKEKVRKKPAEADYDLRRVQNELSKTLETKVDIRQTAQGKGNITIHFVNTEDLNRIL
                     DILEK"
BASE COUNT          414 a          337 c          310 g          456 t
ORIGIN      
        1 tgtaatgcat acagaactcc caaaccaaga cagctgagct cgaagttaga cctgtaataa
       61 tctcttgccg ccctaagtga agcttcattc ggattgagat gtgaaaattc gtttggtttt
      121 ccttccaaaa acaaatctcg ggccttgtcg tatctccggt actccttata attttgttgg
      181 acaaggtatc caacaccacc caaagcaccg tacacgagag ggagcttcca ccagtcgcgg
      241 ttgtagatct gtcctgcccc cggtattatc agagaataaa caagtgcctt tgtaggtttt
      301 ggttggaatt ttcctttagg aatattgagt tgtacggtat ctttagatat gatacgcacc
      361 tgcttatctg caagtgtatc ctgtgcattg cccgaaaagg gcatccaaac catgaaacat
      421 ataagaacca ggaatctaag catctgtctt aattatccgg gaaaattagt tttgaacaat
      481 ttgatagaaa tagcaagcag tatcacaccg aatacctttc ggataatgtt ggtgccacct
      541 tctcccaatt gcctctgcaa ccatccactt atacgtagtg taaagaaaat aaaaactagg
      601 ttcaatacaa taccgatgac aatattgatg acttcgaaag tagtcttaat agaaataagg
      661 gtagtcattg taccggctcc tgctataagc ggaaaagcca ggggcactac cgaaggctta
      721 tcattcatcc cggtatcttt gaacaataca ataccaagta tcatttctat acctatcaaa
      781 aaaagtacaa tggcacctgc cagtgcaaat gattgtacat ccagactcaa caaactcagt
      841 attccttctc ctgcaaacaa gaatccgatc atgatgctac ctgagaccaa tgtaaccttc
      901 agtggctcga tttgtttgcc atctgctttt agcttgatga tgatgggcaa tgcgccgagt
      961 atgtctataa ctgagaacaa tatcagtgaa accgaaaaaa tctgcttcag atccatatat
     1021 tatttttcaa gaatatccag aatacggttt aggtcttcag tgtttacaaa atgtatggta
     1081 atatttccct tgccttgagc tgtctggcgt atatctacct tggtttccag cgtttttgaa
     1141 agttcgttct gaactcttct caagtcgtag tccgcttctg ctggcttttt ccgcactttc
     1201 tctttcggag tctgataatc acgtatgagt gattcggttt ctctaaccga catgcccttg
     1261 ttgcgaacct gattgtacaa gaaaatcatt ttgaccggat cttctatacc tgcgagtgcc
     1321 cgtgcgtgtc ccatactcag ctcttgtgtc ttaagtgctg actgaatttc gggtggcaat
     1381 ttgatgagtc tgaggaaatt ggtgactgtg cttctgtcct tggccacccg ggttgccagc
     1441 gcttcgtgtg tcaggttgca ttcatcgagc aggcgttggt aagtaaaagc aacttccagc
     1501 ggattgaggt cttttcg
//
LOCUS       DAOQQY010000421         1497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_56799_length_1497_cov_5.752427, whole genome shotgun sequence.
ACCESSION   DAOQQY010000421 DAOQQY010000000
VERSION     DAOQQY010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56799_length_1497_cov_5.752427"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLE23_13110"
     CDS             <1..367
                     /locus_tag="PLE23_13110"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxo-5-alpha-steroid 4-dehydrogenase"
                     /protein_id="HMX84253.1"
                     /translation="KVYENQWLTSPEFIIGTLIFVTGLIINWRSDALLIGLRKQDETG
                     YKIPYGGLFKYVSCPNLLGETIEWGGFAILTWSLPGLAFFVWTFANLIPRAIAHHRWY
                     KQHFSDYPEGRKAVIPFLW"
     gene            complement(463..>1497)
                     /locus_tag="PLE23_13115"
     CDS             complement(463..>1497)
                     /locus_tag="PLE23_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HMX84254.1"
                     /translation="IEPVFMPVYKRRRKVNPNDKDARVDFVDEVGDSWEEYVVFHHKF
                     KQWMEVNGYDTQKNYSNDEINELIEKSPYNKATSNDIDWLSKVKMQGAIQKWVDHSIS
                     VTINVPNDTTEELVNKLYMTAWEEGCKGVTVYRDGSRAGVLISNDNKKKDEKQTNDTT
                     KPAFPTKRPQILNADVIRFQNNREKWIAFIGTIDDKPYEIFTGLADDEDGILLPRWVN
                     EGYIIKNKDEFGMSRYDFQYVNQRGYKTTIEGLSYRFNPEFWNYAKLISGTLRHGMDI
                     QNVVELINSLQLDNESINTWKNGVARALKRFIPDGTDAKGKCSDCGSNNLMYQEGCLT
                     CKDCGSSKCG"
BASE COUNT          363 a          299 c          302 g          533 t
ORIGIN      
        1 gaaggtttac gaaaatcaat ggctcacaag tcctgagttt ataattggga cattgatatt
       61 tgttaccggg cttatcatca actggcggtc ggatgcttta cttataggtc ttaggaagca
      121 ggatgaaacg gggtataaga taccttatgg cggtttgttc aagtatgtca gttgtcctaa
      181 tcttttggga gaaacgatag agtggggcgg atttgcaatt ttaacctgga gtttgcccgg
      241 tcttgcattt tttgtctgga catttgccaa ccttataccg agggcgattg ctcatcatag
      301 atggtacaaa caacattttt ctgattatcc ggaaggtcga aaagctgtta ttcccttcct
      361 gtggtagcaa catcaatatg tgcatccaat attttccact aataaaaaaa ggcatttcat
      421 tactgaaaca ccttttctac gaagatttat gttaatatta atttaaccac attttgatga
      481 gccacaatcc ttgcaggtaa ggcatccttc ctgatacatt aggttatttg agccacaatc
      541 agagcatttg cccttagcat ccgtgccgtc cggaatgaag cgtttcagag ctcgtgccac
      601 accatttttc cacgtattaa tagattcgtt atcgagctgt aggctgttga tgagttcgac
      661 tacgttttgg atgtccattc cgtgtcttag tgttccagaa atgagtttgg catagttcca
      721 gaattcggga ttaaatctgt atgaaaggcc ttcgatggta gtcttgtagc ctctttggtt
      781 tacgtattgg aagtcgtacc tcgacatacc aaactcatct ttgttcttaa taatataacc
      841 ttcgttcacc caacgcggta gtagaatgcc gtcttcgtca tctgccaaac cggtgaatat
      901 ctcataaggt ttgtcatcta ttgtgcctat aaatgcgatc catttttctc tgttgttctg
      961 aaaacggatt acatcagcat tcaatatttg tggtcgcttg gtagggaaag ctggtttggt
     1021 tgtatcgttt gtttgttttt cgtccttttt cttattgtca ttagatatta gcacacctgc
     1081 ccttgagcca tcacgatata ctgttacccc tttgcaacct tcttcccagg cagtcatgta
     1141 gagtttgttt acaagttctt cggtagtatc atttggtaca ttgatggtta cagatatcga
     1201 gtggtcaacc catttttgga tggctccctg catttttact ttactgagcc agtcgatatc
     1261 atttgatgtt gctttgttgt atggtgattt ttcgattaat tcattgattt cgtcgttgga
     1321 gtaatttttt tgtgtatcat atccgttgac ctccatccat tgtttgaatt tatgatggaa
     1381 tactacatat tcttcccatg aatcgccaac ttcgtcaaca aaatcaaccc tggcgtcttt
     1441 gtcattcggg tttactttcc ttcttctttt atatacaggc ataaacaccg gttcgat
//
LOCUS       DAOQQY010000422         1489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_57407_length_1489_cov_3.723152, whole genome shotgun sequence.
ACCESSION   DAOQQY010000422 DAOQQY010000000
VERSION     DAOQQY010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57407_length_1489_cov_3.723152"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /gene="dnaK"
                     /locus_tag="PLE23_13120"
     CDS             <1..1489
                     /gene="dnaK"
                     /locus_tag="PLE23_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013768529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMX84255.1"
                     /translation="PAYFNDSQRQATKEAGEIAGLKVKRIINEPTAAALAYGLDKGGK
                     DMTIAVFDLGGGTFAISILELGDGVFEVKSTNGDTHLGGDDFDNVLIDYLADAFKAQE
                     NIDLKKDPMALQRLKDAAEKAKIELSSTQETEINLPYVTAVDGVPKHLVLKITRAKFE
                     QLVDHLVQRSLVPCAKALEDASMSKDKIDEIILVGGSTRIPKIQQAVEEFFGKKPNRS
                     VNPDEVVAIGAAIQGGVLSGDVQDVLLLDVTPLSLGIETMGGVYDVVIEANSTIPTKK
                     SKIYSTAADSQPSVEIHILQGERPMARDNRTVGRFILDGIPPAPRGVPQVEVTFDMDA
                     NGILNVSALEKGTGKKQNIRIEASTGLSKEEIERMKAEAAANAEADKTAKERVEKLNT
                     ADSLIFQTEKQLKEFGDKLPENKKADIEAALKDLKDAHTAQDIAHVERATEALNNAWH
                     AASEDLYKASQEAGAGSAGHAQNGAGQTDSTSDVTDAEFEEVTDK"
BASE COUNT          506 a          295 c          350 g          338 t
ORIGIN      
        1 gcctgcttat ttcaacgact ctcagcgcca ggctacaaaa gaagccggag aaattgccgg
       61 acttaaagta aaacgtatta tcaatgagcc tacagctgca gctttggctt acggtcttga
      121 caaaggcggt aaggatatga ccatagcagt atttgacctc ggtggcggta cattcgctat
      181 ttctatactg gagctgggcg atggcgtatt tgaagtaaaa tcaacaaatg gtgacaccca
      241 cctaggtggt gacgacttcg ataacgtact gatcgattac cttgcagatg cattcaaggc
      301 tcaggaaaac atcgacctta aaaaagaccc aatggcactt caacgattaa aagatgctgc
      361 ggaaaaggca aaaatagaat tatcttcaac acaagaaaca gaaatcaact taccttatgt
      421 gacagccgta gatggtgtgc ctaagcacct tgtactcaaa ataacaagag caaaattcga
      481 acaattggtt gatcatcttg tgcaaagatc attggtgcct tgtgccaaag ctctcgaaga
      541 tgcatcaatg agcaaggata aaatcgatga aatcatactg gtaggcggct ctactaggat
      601 tccgaaaata caacaggccg ttgaagaatt cttcggtaaa aaaccaaaca gaagtgtaaa
      661 ccctgacgaa gtggtagcga tcggtgctgc tatccagggt ggtgtactct ctggtgatgt
      721 gcaggatgta ttgttgcttg acgtaactcc gctctctctt ggtatcgaaa caatgggagg
      781 tgtatatgat gtggttattg aagcgaattc gaccatacct accaaaaagt caaaaattta
      841 ttctacagct gctgacagtc aaccttcggt agaaatacat attctgcaag gagaaagacc
      901 aatggcacga gacaaccgta cagtaggtag atttatactt gatggcattc cgccggcacc
      961 aagaggcgtt cctcaagtag aggttacctt cgatatggat gctaacggta tcctgaatgt
     1021 gtctgcactt gaaaaaggta caggtaagaa gcagaatatc cgtatcgaag cttcaacagg
     1081 attatcaaaa gaagaaattg aaagaatgaa ggccgaggct gccgcaaatg cagaagcaga
     1141 taaaactgct aaagaaagag tcgaaaaact gaatacagca gatagcctga tttttcagac
     1201 tgaaaaacaa cttaaggagt tcggtgacaa attgcctgaa aataaaaagg ctgatataga
     1261 agctgcactc aaagatttga aagatgcaca tacagctcag gatattgcac atgttgaaag
     1321 agcaactgaa gctttgaaca atgcatggca tgctgcaagt gaagacctgt acaaagcatc
     1381 acaggaagca ggtgcaggct cagcagggca tgcacagaac ggagccggcc aaaccgactc
     1441 gacaagtgat gtgacagatg ctgaatttga agaagttacc gacaaataa
//
LOCUS       DAOQQY010000423         1484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_57764_length_1484_cov_4.505248, whole genome shotgun sequence.
ACCESSION   DAOQQY010000423 DAOQQY010000000
VERSION     DAOQQY010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57764_length_1484_cov_4.505248"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PLE23_13125"
     CDS             complement(<1..416)
                     /locus_tag="PLE23_13125"
                     /inference="COORDINATES: protein motif:HMM:NF015291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HMX84256.1"
                     /translation="MKGIVNNLFKWYLSERYGMIERFMRHPVNAQDEVWDYLRTVAED
                     TVFGREYNFASIKDRRSFAQQVPLQDYESLKPYIKRMMYGEKDVLWPGAVEWYSKSSG
                     TTSDKSKFIPVSRENLKKCHIRGSWDTLTILYHNKP"
     gene            complement(504..>1484)
                     /locus_tag="PLE23_13130"
     CDS             complement(504..>1484)
                     /locus_tag="PLE23_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020536985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMX84257.1"
                     /translation="TNRNLIANMLQIRSVMMPFLKDGEEIVLSPLPLYHIFAFTVNCL
                     ALLSVGSYTVLVTNARDLPSVIKEFKKHKISLITGVNTLFNALLNHPDFGSCDFSTLK
                     ISVGGGMAVQKSVAERWQKTTGCPLSEGYGLTETSPVASVNPLDGTGRPGYIGLPVPS
                     TEMRVVLDNGSLAPVGSPGEIQIKGPQVMKGYYNRPDETAKSIKDEWFATGDVGLMEE
                     DGYFKIVDRIKDMILVSGFNVYPNEIEEVAMLNPKVAEAAAIGIADEKSGEVVKLFVV
                     KKDNSLTKEELIEHCRQNITGYKVPKEIEFRDSLPKTNVGKILRRELRDQ"
BASE COUNT          348 a          338 c          330 g          468 t
ORIGIN      
        1 tcgggcttat tgtggtacaa aatagtaagg gtgtcccatg acccgcggat gtgacatttt
       61 ttcaggtttt ctcttgatac cggaatgaac tttgacttat cggatgtggt gccgcttgac
      121 ttcgaatacc attctactgc acctggccaa aggacatctt tctcaccgta catcatcctt
      181 ttaatgtaag gtttgagtga ttcgtaatct tgcagcggaa cctgctgtgc aaaactgcgt
      241 cggtctttga tggatgcaaa attatattcc ctcccgaaaa ctgtatcttc ggctactgta
      301 cgcaggtagt cccaaacttc gtcttgtgca ttgaccggat gccgcataaa tctttcgatc
      361 atgccgtagc gttctgacag ataccattta aacaaattgt tgactattcc cttcatgaac
      421 cggatgtgtg ttttctcaat agtggcaaag ataatgcttt ttgggtgtca ggcaatttct
      481 tcggtattca ttgttttgtt gtttcattgg tctctgagct cacgtcttag gattttaccg
      541 acattggttt ttgggaggct atctctgaat tctatttctt tgggcacctt atagccggtg
      601 atattttgtc tgcaatgttc tatcagttct tctttggtca gagaattatc tttttttact
      661 acaaacagct taaccacctc gccagatttt tcgtctgcta taccaatggc ggctgcttct
      721 gctaccttag ggttcagcat agctacctcc tctatttcat ttgggtaaac attaaagccc
      781 gacaccagta tcatatcctt gatgcggtca acgattttga agtatccgtc ctcttccatc
      841 aggcctacat caccggtagc aaaccattca tctttgattg atttggctgt ctcgtcgggt
      901 ctgttgtagt agcctttcat tacctgtggt cctttgatct ggatttcgcc gggtgagccg
      961 acaggagcca ggcttccatt gtcgagtact acacgcattt cagtagaagg aaccggcaaa
     1021 cctatatacc cggggcgccc tgtaccatcc agcggattga cacttgctac aggtgaagtc
     1081 tcggtaaggc cgtacccttc tgacagtggg caacctgtcg ttttctgcca gcgttctgct
     1141 accgacttct gaactgccat gccaccacct actgagattt tgagtgtact aaagtcacag
     1201 ctgccgaaat cgggatgatt gagtaaggca ttgaacaatg tgtttacgcc tgttataagt
     1261 gagattttat gtttcttaaa ttctttgata accgaaggca ggtcgcgcgc attggtaacc
     1321 agaacagtat atgagccaac actgagcaga gcgaggcaat tgacggtgaa agcaaaaatg
     1381 tggtataatg gcagtggcga caagacgatt tcttcaccat ccttcagaaa aggcatcata
     1441 acggatctta tctgcaacat attggctatc aggttgcggt tggt
//
LOCUS       DAOQQY010000424         1482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_57914_length_1482_cov_6.868255, whole genome shotgun sequence.
ACCESSION   DAOQQY010000424 DAOQQY010000000
VERSION     DAOQQY010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57914_length_1482_cov_6.868255"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="PLE23_13135"
     CDS             complement(<1..735)
                     /locus_tag="PLE23_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMX84258.1"
                     /translation="MRIFLFSVILLLTRATAFSQPDKSLIAYFNFDDCSVNDVVGSGN
                     TASVTGNPACECGVKESGLRFNGLNDEVIVIGTISTVFQNIDFSLSFYFKPLPQTGNQ
                     VIFSKREDCNSDKGFSVNYNTASGTLTAFISESATQQILLSAKTDKDVCWQHVVISRK
                     NRNHYLYINGKLVDEKVSTQRLKIGNNAPLTIANGPCVTETRYKGDLDEIYFYNRAID
                     PSVIQKLDLKPDHIANFDTLIYLGGQV"
     gene            complement(822..1130)
                     /locus_tag="PLE23_13140"
     CDS             complement(822..1130)
                     /locus_tag="PLE23_13140"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HMX84259.1"
                     /translation="MFPNLEEQQKMMQEKLSAQEVLGRSANDAIIVMANAIPEIKSIT
                     IDRTKVNVNESEELEDHLILALNETILQAQAVQAKLSQELISEMLPPGLGSLKNLFGG
                     "
     gene            complement(1137..>1482)
                     /locus_tag="PLE23_13145"
     CDS             complement(1137..>1482)
                     /locus_tag="PLE23_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84260.1"
                     /translation="KSFSPYVSVGVSMIQSADEVQSRFPQAGQIIPEPEDKNFFLSLT
                     FGLGARYDFHEKFTLGAEGIWYPTFSDYLDGVSKNGNADKKDWILSATFYISYFFGAA
                     EPDFNFGKTRKF"
BASE COUNT          408 a          290 c          307 g          477 t
ORIGIN      
        1 aacctgccca ccgaggtata taagtgtatc gaagttggct atatgatcag gttttagatc
       61 caatttctgt attacgcttg ggtcgatggc tctgttgtag aagtaaattt catcaaggtc
      121 gcctttatat cgtgtttcag tcacacatgg tccgttggcg atggtcaaag gtgcattgtt
      181 accaatcttg aggcgttggg tagagacttt ttcgtccact agttttccat tgatatacaa
      241 atagtggtta cggttttttc tgcttatcac tacatgttgc caacatacat ctttgtcggt
      301 tttggcagat aacaatattt gctgggtcgc agattcgctg atgaaggcgg tgagcgtacc
      361 agaggcagta ttataattga cagaaaaacc tttgtcgctg ttacaatcct cgcgttttga
      421 aaaaattacc tgattgccgg tctgtggcaa tggtttgaaa taaaagctta gcgaaaagtc
      481 gatattctga aaaacagtac tgatagtgcc tataactatt acttcatcat taagtccatt
      541 aaatctcagg ccgctttcct tcacaccaca ttcgcaggca gggtttcctg taacagaagc
      601 agtattgcca ctacccacaa cgtcgtttac actacaatca tcaaaattaa aatatgcaat
      661 gagtgatttg tcgggttggg agaaagcggt agcccttgta agtagcagta ttacggaaaa
      721 taggaatatt ctcatcgaag gaatttggtt gtcatgcttt taacgcaatc ctctcagatt
      781 ttgttttaat gctttaagaa gaattgtgcc cgagagcacc attatccgcc aaacaaattc
      841 tttaatgaac caaggccggg tggaagcatt tcagaaataa gttcttgcga taatttggcc
      901 tgaactgcct gtgcctgtaa aattgtttca tttaatgcca agataaggtg gtcttccagt
      961 tcttctgatt cgtttacatt tacttttgtt ctgtcaattg ttattgattt tatttccgga
     1021 atggcgttgg ccattactat gattgcatca ttggcagacc tgcctagtac ttcttgtgca
     1081 gagagcttct cctgcatcat tttctgttgt tcttcgagat tcggaaacat agtttgttaa
     1141 aattttcttg ttttgccaaa attgaaatcc ggttcagctg caccgaaaaa gtatgatata
     1201 tagaaagtgg cacttaatat ccagtctttt ttatctgcgt taccgttttt tgaaacgcca
     1261 tcgaggtaat cgctgaatgt agggtaccaa ataccttcag cacctaaagt aaatttctca
     1321 tggaaatcat acctggcccc aagcccaaaa gtaaggctga ggaaaaaatt tttgtcttcg
     1381 ggttcgggta ttatctgtcc ggcttgcgga aatctgctct gaacctcatc agcagactga
     1441 atcatcgata caccgacaga aacataaggt gaaaaagatt tc
//
LOCUS       DAOQQY010000425         1417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_63157_length_1417_cov_5.684288, whole genome shotgun sequence.
ACCESSION   DAOQQY010000425 DAOQQY010000000
VERSION     DAOQQY010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63157_length_1417_cov_5.684288"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(258..>1417)
                     /locus_tag="PLE23_13150"
     CDS             complement(258..>1417)
                     /locus_tag="PLE23_13150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMX84261.1"
                     /translation="LIFIIPVYVLGKDFDHWLLQAVTLLVIACPCALVISTPVAIYAA
                     IGNASSRGILVKGGKYIEAMARIKAIALDKTRTITFGTPKVGDVLPMYDTSREELLAC
                     TAGAEIFSEHPLAQAIVDASRSEGFEPHSTESFKSIMGKGATAKCLVCEDETIYVGKF
                     DFIKEYHGHDTEAEKHVEQLSSQGKTSVVVSFGKGVAGIIALTDEIKPDSARALRELR
                     ELGIDLAMLTGDSEKAAKYIANQVGINEIYGGLLPENKAEKIAELQKKYQYVAMVGDG
                     INDAPALAASTAGIAMGSAGSDTAIEVANIALMNDRLSLIPFLVRLSRRTLNIIKLNT
                     SGAILIKLLFVTLAFFGYSNLVMAIAADVGVTLLVILISLNLMRFEKKQDM"
BASE COUNT          390 a          298 c          287 g          442 t
ORIGIN      
        1 caaagttagt caattatagt tataattgaa agcctattgc ataattcagg tttaaattcc
       61 ttatgggtta tttgagttta atatttaaca atattcctca aggcaaagtt ccactgcata
      121 tttacaccga tagacttatg aaaaaagtct aagtcaaatt tgctacgatc ttacaactgt
      181 gctgaagaca gattaaagtt aagtatgaaa atttagttac ccattttttt attttataag
      241 tcatgacaaa aaccaactca catgtcttgt tttttttcaa aacgcatcag attaaggctt
      301 atgagtatca ctaacaatgt aacgccaacg tcggctgcaa tggccattac cagattacta
      361 tagccaaaaa atgccagagt gacaaagagt agtttaatca ggatagcacc tgatgtgtta
      421 agttttatta tattaagtgt tcttctgctg agccttacaa ggaatgggat aagagaaaga
      481 cggtcgttca taagggcaat attagctacc tcgatagcag tgtcgctacc tgctgagccc
      541 atggcgatac ctgccgtact cgctgccaga gcgggagcat cgttgatacc gtcaccaacc
      601 attgcaacgt attgatattt tttttgcagt tctgctattt tttcggcttt gttttcgggc
      661 agcagaccgc cgtatatctc gttgatgcca acctgattag caatgtattt tgcagctttt
      721 tcgctatcgc ctgtgagcat agcgagatcg atgcccagtt cgcgcaattc tctgagtgca
      781 cgtgcactgt cgggttttat ttcgtctgtc aatgctatta tgcctgccac gcctttgcca
      841 aagctcacta ctacgcttgt tttgccttgc gaggaaagtt gttctacatg tttttcggct
      901 tctgtatcat ggccgtgata ttctttgata aaatcaaatt ttccaacata gatggtttca
      961 tcttcacata caaggcattt ggcggtagca cctttaccca taatgctttt aaaagattct
     1021 gtactgtggg gttcaaatcc ctcactacga cttgcatcta caatagcttg agcaagtggg
     1081 tgttcggaaa atatttctgc accggctgtg caagcgagca gttcttcacg gctggtgtca
     1141 tacattggca atacgtcacc tactttgggt gtaccaaatg taatggtacg ggttttgtcg
     1201 agagctatgg cttttattct agccatagcc tcaatgtatt ttccgccttt tacaagaata
     1261 ccccttgatg aagcattgcc gatagcggca tagatggcca ccggcgtaga tatgaccaat
     1321 gcacacgggc atgctattac aagcagtgtg acagcctgta gcagccaatg atcaaaatct
     1381 ttgcccaaca catacaccgg aataataaag atgagta
//
LOCUS       DAOQQY010000426         1413 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_63500_length_1413_cov_5.205449, whole genome shotgun sequence.
ACCESSION   DAOQQY010000426 DAOQQY010000000
VERSION     DAOQQY010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1413)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63500_length_1413_cov_5.205449"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="PLE23_13155"
     CDS             complement(<1..411)
                     /locus_tag="PLE23_13155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMX84262.1"
                     /translation="MTDLPVLKIKGLAKHFGHIKAVDGLNLHVSKGEIFGLLGHNGAG
                     KSTTMRMLLSLIKPTSGRIEVFGSELYSNREKILSDIGSIIEKPDFYPWLSAKTNLEL
                     FAALSRKPVSKNRISQTLEFVGLAARAGDKVKTYS"
     gene            601..>1413
                     /locus_tag="PLE23_13160"
     CDS             601..>1413
                     /locus_tag="PLE23_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002658415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMX84263.1"
                     /translation="MKIYRKSIIPLLLAVWTIAAITSLKAQLTGNLQQNSPIPFHESV
                     RTGTLSNGMKYYLLKNAKPEHRVEIRLALKAGSILEDEDQKGVAHFVEHMCFNGSKNF
                     KKNELIDYLEKTGTKFGADLNAYTSFDETVYMLELRTDDQAIFNKGLLVFEDWAGGVN
                     FDHEEIDKERGVVESEWRTRLSPEQRMQNRYFPVIYAGSQYGKRLPIGDMEIIRHAPY
                     DNFKRFYRDWYRPDLMALIMVGDFDLNAVEKQVKERFSKLKNPAKPRPRTEFD"
BASE COUNT          416 a          268 c          329 g          400 t
ORIGIN      
        1 gctataagtt tttaccttat cacctgctct ggcagcaagc ccgacaaatt cgagtgtttg
       61 ggatattctg ttttttgaaa ccggttttct gctcagtgca gcaaagagtt cgaggttggt
      121 tttggcactc aaccaggggt aaaagtccgg tttttcaatg attgaaccga tgtcggagag
      181 aattttttct ctgtttgaat acaattcact gccaaaaact tctattctgc ctgatgttgg
      241 tttaatcagt gagagcaaca tgcgcatagt ggtacttttg cctgcaccgt tatggccaag
      301 gaggccgaat atttctcctt tgctgacatg taggttgagt ccgtcaactg ctttgatgtg
      361 gccaaagtgt ttggcgagcc ctttgatttt taaaaccggt aaatcagtca tctgtgaatc
      421 atttctgtgt gtagaaatac ggaatatttt atccgggttg aacaaaaaat gtgtgcaata
      481 atatcaactt tataggataa aaaccgattt tggtagaaaa ttttggactt acaaaaataa
      541 attcggggat gtgtggttta ttatttgtat tttagggcaa taataagaaa acatttgaat
      601 atgaaaatct accgaaaaag tattattccg ttattgcttg cggtgtggac catagcagct
      661 ataacatcgc tgaaggctca gcttaccggc aatcttcagc agaattcgcc tataccattt
      721 cacgaaagtg tgcgaacagg tacattgtct aatggtatga aatactacct gctcaagaat
      781 gccaaacccg aacaccgggt agagattagg ctcgccctca aggcaggctc cattcttgaa
      841 gacgaagacc agaaaggtgt ggctcacttt gtagaacaca tgtgctttaa tggttcgaaa
      901 aatttcaaaa aaaatgaact gattgattac cttgaaaaaa ccggaaccaa atttggtgct
      961 gacctaaatg cctatacttc atttgacgaa acagtgtata tgctcgagct tcgaacagac
     1021 gaccaggcga tattcaacaa gggcttactt gtctttgaag actgggcagg tggcgtcaat
     1081 tttgatcatg aagaaataga taaagaacgt ggtgttgtgg agtcagaatg gcgcactcgt
     1141 ctaagtccgg agcagaggat gcagaacagg tacttcccgg ttatctacgc aggctcacaa
     1201 tacggcaaga ggcttcctat tggtgatatg gaaatcataa ggcatgcacc gtatgacaac
     1261 ttcaaaaggt tttaccgcga ctggtatcgt ccggacctta tggccttgat aatggttggc
     1321 gacttcgact tgaatgctgt agaaaagcag gtaaaggaga ggttctcaaa actgaagaat
     1381 ccggcgaagc ccaggccacg aacagagttt gat
//
LOCUS       DAOQQY010000427         1400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_64670_length_1400_cov_3.266914, whole genome shotgun sequence.
ACCESSION   DAOQQY010000427 DAOQQY010000000
VERSION     DAOQQY010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64670_length_1400_cov_3.266914"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLE23_13165"
     CDS             complement(<1..235)
                     /locus_tag="PLE23_13165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020538701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX84264.1"
                     /translation="MKLIIAGGGTGGHVFPAIAIANAVRALRSDTEILFVGAKDKLEM
                     EKVPAAGYKIEGLWISGFQRNLSLKNLSFPLKLA"
     gene            complement(232..>1400)
                     /locus_tag="PLE23_13170"
     CDS             complement(232..>1400)
                     /locus_tag="PLE23_13170"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HMX84265.1"
                     /translation="SSSMGLAFKNNSTAHLYLLQHFLILSVGFVIIYIVSLMNHIKFS
                     TIGVALMILAVLLLVYTLFFGSSINGASRWIRIPIINITFQTSDFAKLALVIYLARIL
                     TTKQEKIKDFKAAFVPILFPVAIVCFLIAPFNLSTAMLIFLTSMVMMFIGRVRVKFMA
                     GLAAMGVGMLGLLYILWWFFPNHVRVQTWSNRIQDFMANGDGSFQNVQAKIAIANGGF
                     FGAGVGDGGHLRFLPSAYADFIYASICEEYGVFGGAIILILFVWFFIRVVVLVAKSPK
                     TFGTLLAIGLALLITVQALTNMAVSVHLLPVTGLTLPLVSKGGTSLIFTSIALGMILS
                     VSRYVEKSTKSAIKETNKNEAVVSENDEVNVTEAVPPFDIAYVVDDTKKGTNIV"
BASE COUNT          465 a          323 c          253 g          359 t
ORIGIN      
        1 gtgcaagctt cagcggaaag cttaaatttt ttaagctcaa gtttctttga aatccgctaa
       61 tccacaagcc ttctatcttg tagccagctg cgggcacttt ttccatttcg agcttatctt
      121 ttgctccgac aaatagtatc tcagtatcag acctgagagc tctgacagca ttagctatag
      181 ctatagccgg aaagacatgg cctccggtac ctccgcctgc gataatcagt ttcatactat
      241 gttggtgcct tttttagtat catcaactac atacgcaata tcaaaaggtg gaactgcctc
      301 tgttacattc acttcatcat tttcagatac gacagcttca tttttgttcg tctctttaat
      361 agcagatttt gtagattttt caacgtatct ggatacactc aaaatcatcc ctaaagcaat
      421 acttgtaaaa atcaatgatg tgccaccttt agacaccaaa ggcaatgtaa gccctgtaac
      481 gggcaacaaa tggacggaca ccgccatatt ggtcaaagcc tgtacggtaa tgagcagtgc
      541 tagtcctatg gccaaaaggg ttccgaaagt tttcggactt tttgcaacaa gtacaacgac
      601 ccgtataaaa aaccaaacaa ataatatcaa tataatagca ccaccaaaca caccatactc
      661 ttcacaaata gatgcgtata taaagtcagc ataagccgaa ggcaggaacc gaagatgccc
      721 gccgtcaccg acaccggcac caaagaatcc accattggct attgcaattt ttgcctgtac
      781 attctggaac gaaccatcac cattcgccat aaaatcctga attctgttcg accaggtttg
      841 taccctgacg tgattcggga aaaaccacca caaaatgtat aaaagcccaa gcataccaac
      901 tcccattgct gccaggccag ccataaattt tacccttacc ctgccgataa acatcattac
      961 catggaagta aggaatatta acattgcggt agaaagatta aaaggggcaa tcaaaaaaca
     1021 aacaatggca accgggaaca agatcggaac aaatgcagcc ttaaagtctt taattttctc
     1081 ctgcttggta gtgagtatcc ttgccagata gatgacaagt gcaagtttag caaaatcaga
     1141 agtctggaaa gtaatgttga tgatcggtat cctgatccat ctcgaagcac cattgataga
     1201 cgaaccgaaa aacaaagtat aaaccaacaa caacacagcc aatatcatca aagccacacc
     1261 gattgttgaa aatttgatgt gattcatgag tgatactatg tagatgatca caaaccctac
     1321 agacagaatg agaaaatgct gcagcaagta caaatgtgct gttgaattat ttttaaatgc
     1381 aagccccata gatgagctgt
//
LOCUS       DAOQQY010000428         1396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_65009_length_1396_cov_6.466816, whole genome shotgun sequence.
ACCESSION   DAOQQY010000428 DAOQQY010000000
VERSION     DAOQQY010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65009_length_1396_cov_6.466816"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..1301
                     /locus_tag="PLE23_13175"
     CDS             75..1301
                     /locus_tag="PLE23_13175"
                     /inference="COORDINATES: protein motif:HMM:NF025341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HMX84266.1"
                     /translation="MVWRRIFFLILYFNTILISWGQVPVFRFLHNRGHVVIPFELQYN
                     FIIVKVKVNEKYTMRFIVDTGAEYSAVNQTAADLINIKKGRKVNVMGTDHRTILIAHV
                     GNGISLRMDDFLIDNYNVLIFPEEAPQFEVLNHLQIAGIIGADVLSRYLVKFDFKRQE
                     MSLMSHSKTRIPKDYSNIPLMLHKNRAFVQIPVVGNARDTTQLSLLFDTGAGLPLLLK
                     DNGKGRIPIPENLVDGKIGLGVGGYIEGTIGKISAVQLGGHTLYNVLTSFQEITNPPD
                     SVKLPTSDGILGNDIIRLFTWYIDYRLEKCYLKPNRDFKKAPQYDKSGMTVLMSGANL
                     NHYVVIGVFPGSPAAEADIRIGDQIISVNNRRGIFLSFNTIVKTLSKNEGETVHLKLK
                     REGEKLYKTIVLKNIL"
BASE COUNT          458 a          238 c          277 g          423 t
ORIGIN      
        1 tttaatctta taaatgtaag aaaataattt aataattagc tacattagag tacgataact
       61 caatttttat gttgttggtt tggcgcagaa ttttctttct catattgtat tttaatacaa
      121 ttttaataag ctgggggcag gtacctgtat ttcgtttttt gcataaccgt gggcatgtag
      181 tcattccgtt cgagctacaa tacaatttta tcattgtaaa agtgaaggtg aatgaaaaat
      241 ataccatgcg ttttatcgtg gatactggcg cagaatattc agctgtcaac caaaccgctg
      301 cagacctgat aaatataaaa aaaggcagga aagtaaatgt gatgggcaca gatcatcgaa
      361 cgatactcat cgctcacgta ggcaatggta taagcctaag gatggatgat ttcttaattg
      421 acaattataa tgtacttatt tttcctgaag aagcaccaca gtttgaagtg ttgaatcatt
      481 tgcagatagc aggcataatc ggcgctgatg tattgagccg gtatttagtg aaatttgatt
      541 tcaaaaggca ggagatgagc ttaatgtcgc attctaaaac cagaataccg aaagattatt
      601 ccaatattcc tttgatgctg cacaaaaacc gtgcttttgt acaaattcct gtcgttggca
      661 atgcacgcga tactacccaa ctgagtttat tgttcgatac tggcgcaggc cttccattgc
      721 tattgaagga taatggaaaa gggcgaatac cgataccgga aaatcttgtc gatggaaaga
      781 ttggcctggg agtgggtgga tatatcgaag gtaccattgg aaaaatttca gccgtacaat
      841 taggcgggca tacattatat aatgtattga caagctttca agaaatcacc aacccgcctg
      901 actcggttaa actcccaact tctgacggca tcttgggtaa tgatattatc cggttattca
      961 cctggtacat tgactaccgg ctcgaaaaat gctaccttaa accaaatcgt gattttaaaa
     1021 aagcacctca atacgacaaa agtggcatga ctgtactcat gtcaggagcc aacctcaatc
     1081 attatgtagt gattggagtt tttccgggta gcccggctgc agaagcagat atacgtattg
     1141 gtgatcagat tatttctgtc aataaccgta gaggaatatt cctttcgttt aatacaatag
     1201 ttaagacact cagtaaaaat gaaggcgaaa cggtacacct gaaacttaaa agagaaggag
     1261 aaaaactata caaaaccata gtattgaaaa atattctgtg aaatttatat attttacttt
     1321 gcatttttac aataaaggag taactttgca cgcttaaatt taaaaatcat aaattatgtt
     1381 tgtagttgta tctatt
//
LOCUS       DAOQQY010000429         1380 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_66477_length_1380_cov_6.569057, whole genome shotgun sequence.
ACCESSION   DAOQQY010000429 DAOQQY010000000
VERSION     DAOQQY010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66477_length_1380_cov_6.569057"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PLE23_13180"
     CDS             <1..344
                     /locus_tag="PLE23_13180"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HMX84267.1"
                     /translation="LIGLYLVILLYILNPGLGEMKIPVMVYAVVISYMLLNAVWASRA
                     MQSRPANQLILGAVSFVVSDSILAINKFHTPFDAASFLIMVTYLFAQYSIVSAWLSSS
                     LDEVVQDKHSF"
     gene            355..1302
                     /gene="queG"
                     /locus_tag="PLE23_13185"
     CDS             355..1302
                     /gene="queG"
                     /locus_tag="PLE23_13185"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HMX84268.1"
                     /translation="MHNTIQTRLIHYAHELGFQNIGFSQAGRLDTAAERLEAWLRGGH
                     AGNMSYLENNFDKRVDPTQLVPGAKTVISLSYNYYNPDKQADPDAPKISMYAFGRDYH
                     KVVKKKLKLLFEYLQQIADKPVEGRYFTDSAPVMESNWAAQAGLGWKGKHTLLITPQV
                     GSYFFLAEMIVDYDFESTHAIKDHCGTCTRCIDACPTDAISKQGYILDGSRCISYLTI
                     ELRDNIPEEYKDKLEGWMFGCDICQQVCPWNRFAKRHNEPDFEPHKDLLQMTKNDWQD
                     LREDIFNEILQRTPVSRAKYEGLKRNIRFLTIEDTTCQD"
BASE COUNT          416 a          288 c          303 g          373 t
ORIGIN      
        1 gcttgattgg tctgtatttg gtaattttgc tatatatttt gaatccgggt ttgggcgaaa
       61 tgaagattcc ggtcatggtt tatgcggttg tcattagtta tatgctgtta aatgcggtat
      121 gggcatcccg tgctatgcaa tcccggccag caaaccaact gatattgggt gctgtatcct
      181 ttgttgtatc ggacagcata cttgccatca acaaatttca cacacccttt gatgcagcgt
      241 catttttgat tatggtcacg tatctgtttg ctcaatattc gattgtatca gcttggcttt
      301 cttcttcact tgatgaagtt gtacaggaca aacattcatt ttaataaact tattttgcat
      361 aatacaatac agacacggct tatacattac gcacacgaac tcgggtttca gaacattggg
      421 ttttcacagg caggaaggct cgataccgca gcagaaaggt tggaggcgtg gctccgtggc
      481 ggtcatgcgg gcaatatgtc gtatctcgag aataattttg ataagagagt ggacccgaca
      541 caacttgtac ctggggcaaa gacagtcatt tctttaagct acaattatta caatccggat
      601 aaacaggccg acccggatgc cccaaaaata tcgatgtatg ccttcggacg cgattaccat
      661 aaagttgtaa agaaaaaatt gaagctccta ttcgaatatc tgcagcaaat agcagacaaa
      721 cccgtggaag gaaggtattt tacagactca gcacccgtaa tggaaagcaa ttgggctgcc
      781 caggccggct taggatggaa aggcaaacat accctgctga ttacgccaca ggtcggatca
      841 tattttttcc ttgccgaaat gattgttgat tatgatttcg aatcgacaca tgccatcaaa
      901 gaccattgcg gtacttgtac acgttgtata gatgcctgcc ctactgatgc catctcaaaa
      961 caaggctata tactggacgg gtcgcgttgt atttcatatc ttactattga gctacgcgac
     1021 aatatacccg aagaatacaa ggacaaactc gaaggctgga tgttcggctg cgacatttgt
     1081 cagcaagtgt gcccatggaa ccgattcgcc aaaaggcaca acgaacccga tttcgaacca
     1141 cacaaggacc ttctacaaat gacaaaaaac gactggcagg atcttagaga ggatatattc
     1201 aacgaaatac ttcagcgcac accggtgagc agggcaaaat acgaaggatt gaaaagaaat
     1261 attcgtttcc ttacaataga agataccacg tgccaagact aactgccaaa atattgcagg
     1321 tatataatca atgtcgttta gttaagcgaa aatacggata aaaatcttaa atttgtgatg
//
LOCUS       DAOQQY010000430         1355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_68869_length_1355_cov_3.863846, whole genome shotgun sequence.
ACCESSION   DAOQQY010000430 DAOQQY010000000
VERSION     DAOQQY010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68869_length_1355_cov_3.863846"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1339)
                     /locus_tag="PLE23_13190"
     CDS             complement(56..1339)
                     /locus_tag="PLE23_13190"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020571914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMX84269.1"
                     /translation="MKVFKFGGASLKDAEGIRTVGSINGLHTDTELVVVVSATGKSTN
                     LLEKVVYDYYHQTGNPQQYVDEFELTHLSIIKDLFDPEHNIFHQLNKVSSDLKSILST
                     KPNASYDQIYDSIVSAGELLSSYIVHAWLNHSGIKTAWLDARSVIKTDENFREGRILW
                     NDTLAASKSIIPPLFEESKVVVTQGFLGGTLRGQTTTLGREGSDYSAAIISFCLDAEN
                     QTIWKDVPGVLNADPRKFENAILIDKLSYREAIEMTYYGASVIHPKTIQPLQRKNIPL
                     LVKSFINPEGEGTLISADGNSIYPPVVVLEMNQILLQISTRDFSFVAEHHLSDIFKKF
                     ADVRVKVNMMQNSAISFSACITNQADKVDRLLASLRDQFKVDADDDLELLTIRHYNDS
                     ILHELKRNRIIILEEKLSDTVQMITKRVPQLKMKA"
BASE COUNT          375 a          265 c          287 g          428 t
ORIGIN      
        1 gcgacatgaa gataatggaa gagcgaaatg atttgttttg aaattagttc tacctctaag
       61 ccttcatttt tagctgcgga actcgctttg taatcatttg tactgtatct gacaactttt
      121 cctctaggat aatgatgcgg ttcctcttga gttcatgcag gattgaatcg ttataatgcc
      181 taatggtaag caactcaaga tcgtcatctg catctacttt aaactggtcg cgcaatgaag
      241 ctaacaacct gtcaacttta tcggcttgat tagtgataca tgcagagaaa ctgatagcac
      301 tgttttgcat catgtttact ttaacacgta catcggcaaa cttcttgaat atatcactca
      361 gatggtgttc agctacaaaa gaaaaatcgc gtgtggatat ctgcagtagt atctggttca
      421 tttcaagtac taccaccggt gggtatatac tgttgccatc tgcacttata agcgtacctt
      481 caccttcggg gttaataaac gatttgacca acagtggaat gttttttctc tgcaatggct
      541 gaatcgtttt tgggtgaata acggaggcac cataatatgt catttcaatg gcttcgcggt
      601 agctgagttt atcaataaga atggcatttt cgaattttcg cgggtcagca ttgagtacgc
      661 ccggcacatc tttccagatg gtctggtttt ctgcatcaag gcaaaatgaa ataatggcag
      721 cactatagtc tgagccttct cggccgagtg ttgtagtttg gccacgtagc gtgccaccta
      781 aaaatccttg tgtgacgact acttttgatt cctcaaacaa gggtggaata atacttttgg
      841 aagcagccaa tgtatcattc caaagtattc ggccttcacg gaagttttca tctgttttga
      901 ttacactgcg tgcatcgagc caggcagttt tgatgcccga gtgattaagc caagcgtgca
      961 ctatgtacga agacaataat tcgccggctg agacaatgct gtcataaatc tgatcataag
     1021 aagcattggg ctttgtagag agtatagatt ttaagtcgga tgagacctta ttgagttgat
     1081 gaaaaatatt gtgttccggg tcaaacaaat ctttgatgat gcttaggtgt gtaagctcga
     1141 attcatcaac atattgctgt gggttgccgg tttggtggta ataatcatat accacttttt
     1201 caagcagatt ggttgatttg ccggttgcag aaactacgac aaccaactct gtgtcagtat
     1261 gtaaaccatt aatagaacca acagttctta taccttcggc atcctttaat gatgcacctc
     1321 caaatttgaa tactttcata ttataacatc gttac
//
LOCUS       DAOQQY010000431         1339 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_70422_length_1339_cov_3.921340, whole genome shotgun sequence.
ACCESSION   DAOQQY010000431 DAOQQY010000000
VERSION     DAOQQY010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70422_length_1339_cov_3.921340"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLE23_13195"
     CDS             complement(<1..582)
                     /locus_tag="PLE23_13195"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMX84270.1"
                     /translation="MLHKIFTLLILAIGTVTFAQVDLEVMVLDYSYNKPVAGIEVKLV
                     NESTGFHLARTTGVNGRVVFEGIPVTDKYVVSVDETEAYYASEVNDIKLQSNTNASVT
                     INIGSKINIHLSEIVIRGSSNINIRNAEVSSVLKEKEIQSLPLEGRDITRLLYRLPNV
                     VQATGFYPEAPNVSINGANSLFTNYLIDGMDNNE"
     gene            complement(659..>1339)
                     /locus_tag="PLE23_13200"
     CDS             complement(659..>1339)
                     /locus_tag="PLE23_13200"
                     /inference="COORDINATES: protein motif:HMM:NF021365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HMX84271.1"
                     /translation="QTAILFFSRSARAECQYKILDKQLPYTHNLKLIKQNISAAYTKI
                     VDSGLPFFVFDENRQSGNSFGEKLSVAARECFGFGYKHLIIVGNDCPDLEASDMQLAA
                     VQLQSGKSVLGKDKRGGFYLVGISKDLFDAQVFEQLPWSTPCLQDAMACWLTELGKEI
                     LFLDEKSDINTTSDYDNISDSYILRFYRNINSQFSFYFKRNIQPTDFELLFGFLNIFK
                     LRGPPFFG"
BASE COUNT          394 a          265 c          263 g          417 t
ORIGIN      
        1 ttcattgtta tccataccgt caatgagata attggtgaag agcgaatttg caccattgat
       61 actcacatta ggtgcctctg gataaaaacc agtagcctga acaacattcg gcaagcgata
      121 caatagtcgt gtgatgtcgc gcccttcaag tggcaggctc tgtatttcct tttccttcaa
      181 tacagacgat acttcagcat tccggatgtt gatatttgaa gaacccctga tgacaatctc
      241 gctgagatgt atatttattt ttgatccgat gttaatcgtc accgatgcat tggtgttgct
      301 ttgaagttta atatcattga cttcagatgc ataatatgcc tctgtttcat caactgagac
      361 gacatatttg tcagtgaccg gaataccttc gaacactact cgcccattaa cgccggtagt
      421 acgtgccagg tggaagcctg tactttcatt caccagcttt acttctatgc cggctaccgg
      481 tttgttgtat gaataatcga gtaccatgac ttcgaggtca acttgtgcaa acgttactgt
      541 gccgattgcc agtatcagca atgtgaatat cttatgaagc atattgataa taatttggat
      601 aaaataattg aatgattatc tccggtagcc atttccgggg gaaggactta tgtaaatttt
      661 acccaaaaaa tggtggccca cgtagcttaa aaatgtttag gaagccaaat agaagttcaa
      721 agtcggttgg ctgaatattt cttttaaaat aaaatgaaaa ttgactgtta atatttctat
      781 aaaacctaag aatatacgaa tcagatatat tgtcgtaatc tgatgtcgtg ttgatgtctg
      841 atttttcatc aagaaaaagg atttccttgc caagttcagt gagccaacat gccattgcat
      901 cctgaagaca aggtgttgac cacggaagct gttcgaaaac ttgggcatca aagaggtctt
      961 tgcttatacc aacgaggtaa aaacctcctc ttttgtcttt acccaaaacc gatttgcctg
     1021 attgtagttg tactgctgca agctgcatgt cgctcgcttc caagtctgga cagtcattgc
     1081 ctacaatgat caaatgcttg tagccaaaac caaaacactc acgtgcagca actgataatt
     1141 tttcaccaaa actattgcct gattgcctat tttcatcaaa aacaaaaaaa ggaaggcccg
     1201 aatccactat tttcgtatag gctgccgata tgttctgttt aattaacttt agattgtgtg
     1261 tataaggtag ttgtttgtca agaattttgt attgacattc tgccctggca gagcgtgaaa
     1321 aaaataatat agccgtttg
//
LOCUS       DAOQQY010000432         1332 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_71171_length_1332_cov_2.649961, whole genome shotgun sequence.
ACCESSION   DAOQQY010000432 DAOQQY010000000
VERSION     DAOQQY010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71171_length_1332_cov_2.649961"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLE23_13205"
     CDS             complement(<1..587)
                     /locus_tag="PLE23_13205"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HMX84272.1"
                     /translation="MKDFSWLSRTEALIQKEKVELLAKQHVLVVGMGGVGSYAAEFLV
                     RAGIGSLTIVDGDNVDPTNRNRQLPAMKSTEGKLKTEVMQARLLDINPDLNLTIITKF
                     LNPEDTAQIVNNEYDYIADCIDSITPKLNLITGALKAGKRIVSSMGAGGRLDPTKIQV
                     ADISKTYNCRLAFTIRKRLKDMGVRKGFKAVFSSE"
     gene            complement(574..1230)
                     /locus_tag="PLE23_13210"
     CDS             complement(574..1230)
                     /locus_tag="PLE23_13210"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMX84273.1"
                     /translation="MYINLHTHLPTQNDIEIYNIDIRQPEIEKYPGYYSLGIHPWWIE
                     QIDVDEMLAFIEVQMTDQNFLAIGEIGLDKLADTNFDLQVKVFKKQAILAIKYAKPLI
                     IHAVKTHFDIRQILNKMKFGQPVIFHGFNQNAHIAAGLNQPGNYFSLGTALLNENSNA
                     ADFLKNADLTTIFFENDDKETDIKHIYHAASELLNVEINELCKLIENNFKTIFLHERL
                     "
BASE COUNT          327 a          267 c          289 g          449 t
ORIGIN      
        1 aactcagaag agaaaaccgc tttgaatcct ttcctgacac ccatgtcctt taaccgtttt
       61 cgtatggtaa aagcaagcct gcaattatat gtttttgaaa tatctgctac ttgtattttt
      121 gttgggtcga gtctgccacc ggcacccatc gagctgacga tgcgtttccc ggctttcaat
      181 gcaccggtta tcagattgag tttgggtgtt atgctgtcga tacagtcagc aatgtagtca
      241 tattcgttgt tgactatttg ggctgtatct tccggattca ggaatttggt gatgattgtc
      301 aggttcaaat cggggttgat gtccagcagc cttgcctgca taacctcagt cttgagtttg
      361 ccttcggttg acttcatagc aggcagttgt ctgttgcggt ttgtagggtc aacattgtca
      421 ccatctacaa tagtaagtga tccgataccg gctcttacaa gaaactcggc tgcgtaagag
      481 cctactccgc ccatgcctac cactagcacg tgttgcttag ccagcagttc tactttttct
      541 ttttgtatca atgcttctgt ccggctgagc cagctaaagt ctttcatgta aaaatatcgt
      601 tttgaaattg ttttctatca gcttgcaaag ttcgtttatt tctacattca atagttcact
      661 tgcagcatgg taaatgtgct tgatgtctgt ctctttgtca tcgttttcga aaaaaatggt
      721 ggtcaagtca gcatttttta aaaaatcagc agcattcgaa ttttcattta acaaggcagt
      781 gccaagcgag aagtaatttc caggttggtt aaggcctgct gcaatatggg cattttggtt
      841 gaatccgtga aatattacgg gttgtccgaa tttcattttg ttcaatatct gccgaatgtc
      901 aaaatgtgtt tttacggcat gaatgataag tggtttagcg tatttgattg ccagtatggc
      961 ctgttttttg aacactttca cctgtaggtc aaaattagtg tcggccaact tgtcgaggcc
     1021 tatttcgcct attgccagaa agttttgatc ggtcatctga acttcgataa aggctagcat
     1081 ttcgtccaca tctatctgct ctatccacca aggatgtatg ccaagtgaat agtagcccgg
     1141 atatttctct atttccggtt gtcggatatc gatattgtat atctcgatat cattttgtgt
     1201 cggtaaatgt gtatgcaggt tgatatacat tcagaagatt tgttttcaga ataagatacc
     1261 tgcaatggta gctgaaagat acgaagcgag tgtaccacag atgagtgcct tgaacccaag
     1321 ctttgcaata tc
//
LOCUS       DAOQQY010000433         1329 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_71417_length_1329_cov_4.124804, whole genome shotgun sequence.
ACCESSION   DAOQQY010000433 DAOQQY010000000
VERSION     DAOQQY010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71417_length_1329_cov_4.124804"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..>1329
                     /locus_tag="PLE23_13215"
     CDS             161..>1329
                     /locus_tag="PLE23_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015693632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMX84274.1"
                     /translation="MKIVKLLTAVLLTAFLTPSLSAQILNPVKWKFESKQISGDQHEL
                     IMTATIDEGWTIYSQYLESDDGPVPTTFEFDKSKSYKREGKVTEDKLNRKETHDPIFD
                     MKVVKFAKKAVFRQKVNITDYSKPITGYVDFMTCDAEKCLPPTKVDFNIKIEKPADSK
                     IGTTAATTPDTEKEEPDTTELAATNTSPGDTAVTKSFSTGPSCYAKVTEGNKLDQRIA
                     SVMESYEKPLGDCGKEAVTRDLGLLWTFLFGFLGGLIALLTPCVFPMIPLTVSFFTKG
                     SKDRKSGMRNGILYGLSIIVIYVTIGLLITAIFGATALNALSTNWIANTLFFLIFLFF
                     AFSFFGYYEITLPSWLANKSDAMADKGGHFGIFFMAFTLAIVSFSCTGPIIGSALV"
BASE COUNT          408 a          295 c          260 g          366 t
ORIGIN      
        1 atcagatggt catcaagcaa cgtataaaaa ccaatacgcc cattgaatct ctaagtggtc
       61 atatagaatt catgacttgt aataatgaag tctgctaccc gccggctgaa gtatatttta
      121 aaaccccgat taattgaccc agctaaaaaa ctataatcaa atgaagatcg tgaagttact
      181 gacggctgtt ctgctaacag catttctgac tccatcgtta agtgcacaaa ttctcaatcc
      241 tgtcaaatgg aaatttgaat caaaacaaat ctccggtgat caacacgaac tgatcatgac
      301 agctaccatt gatgaagggt ggaccatcta ttcgcagtat ctggaaagcg acgatgggcc
      361 ggtacctact acatttgagt ttgataaatc aaaatcctac aaacgcgaag gcaaggttac
      421 agaagacaag ctgaacagaa aggaaactca tgacccgatt tttgacatga aggtcgtcaa
      481 atttgccaaa aaagccgttt ttcgccaaaa ggtaaatatt actgattatt caaaaccaat
      541 aaccggttat gttgatttca tgacctgtga tgcagaaaaa tgcctgccgc caacgaaagt
      601 ggatttcaac attaaaatcg aaaaaccggc tgacagtaaa ataggtacta cagctgctac
      661 cactccagac acagaaaaag aagagcctga tactaccgaa ttggcagcta ccaatacatc
      721 accgggcgac acagccgtca cgaaaagttt ctccacagga ccgtcatgct atgcaaaagt
      781 aacagaaggc aacaaactcg accagcgcat tgcatctgtg atggaatctt atgaaaaacc
      841 attgggtgac tgtggcaaag aagctgttac acgtgacctc ggactattgt ggacattcct
      901 gttcggtttc ctgggtgggc ttattgcttt gcttacacct tgtgtcttcc cgatgattcc
      961 gcttacggta agctttttca ccaaaggcag caaagaccgg aaatccggta tgcgcaacgg
     1021 tattctttat ggcctatcca ttatagtaat atatgtgaca ataggtttgc tcattactgc
     1081 aatattcggt gccacagcat tgaatgcact ctctaccaac tggattgcca ataccttatt
     1141 ctttttaata ttcctgtttt ttgcattttc atttttcggt tattacgaaa taacactccc
     1201 atcctggctt gccaataaaa gtgacgcgat ggctgacaaa ggcgggcact ttggtatttt
     1261 cttcatggcg tttacacttg ccattgtttc attctcttgt acaggtccta tcattggttc
     1321 agcacttgt
//
LOCUS       DAOQQY010000434         1311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_73345_length_1311_cov_5.890924, whole genome shotgun sequence.
ACCESSION   DAOQQY010000434 DAOQQY010000000
VERSION     DAOQQY010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73345_length_1311_cov_5.890924"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLE23_13220"
     CDS             complement(<1..196)
                     /locus_tag="PLE23_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84275.1"
                     /translation="MKAAIVIPVYKPIPDAAEQMSLDQTAAIFKGEAIFFVGPATLDY
                     SYYQTKIAESTIIQFEDTYFG"
     gene            complement(193..>1311)
                     /locus_tag="PLE23_13225"
     CDS             complement(193..>1311)
                     /locus_tag="PLE23_13225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMX84276.1"
                     /translation="NFGNQQNAGYHSKVRAQAGALRKTGLDVDLLYFRNYECVIESEN
                     SEKKYPFSNRLSLLWFMFITYPITSGANGYQGLYIRHFLTNPLFLVMLLIYKLKKTSI
                     VLELPTFPYAFEYKEWNKNRLLYLIDRCCSMFFRFFISRIVTFSFDDKIYGIKTIRTD
                     NGVEVSKIKAAQILPDFNNELSLLGLGNPRIWHAYERIIEGMKIFYQENPSIKVKFEI
                     AGQGGELEKYRKLVDDYKLNEYVIFHGFQTGKNLDDLCERCHVAVASLGMHRINVAKG
                     EASPLKAREFAARGIPFVTAYTDKGFPVGFPYILQFPADESPIDISKIVAFYRELRNT
                     LPQYQTDLRQYAEDNLDWSAKMKTVADYFKQQTKTKNN"
BASE COUNT          370 a          270 c          252 g          419 t
ORIGIN      
        1 acccaaagta agtgtcttca aactgtatga ttgtgctttc agctatcttt gtctgataat
       61 agctgtagtc aagcgttgcc ggaccgacaa aaaaaatagc ctcaccctta aaaatcgctg
      121 ctgtttggtc aagcgacatc tgctctgcag catcaggtat gggcttgtaa acgggtatga
      181 caattgcagc tttcaattat tttttgtttt agtttgttgt ttgaaataat cagctactgt
      241 tttcatcttt gctgaccagt cgagattgtc ttcagcatat tgtctcaggt ctgtttgata
      301 ttgtggcaat gtattccgaa gctccctata aaatgctaca attttgctta tatcaatcgg
      361 tgattcatca gccggaaact gcaaaatata aggaaaacca accggaaatc ccttatctgt
      421 ataggccgta acgaatggaa tacctcgtgc cgcaaactcc ctggctttca aaggtgaagc
      481 ttcacctttg gccacgttaa tgcggtgcat acctaagctg gcaactgcta catggcagcg
      541 ttcgcacaag tcgtcaagat ttttaccggt ctgaaaaccg tgaaaaatga catattcgtt
      601 gagtttgtaa tcatctacga gtttgcggta tttttcgagt tctccgccct gtcctgcaat
      661 ttcaaatttc accttaatag aaggattttc ttggtaaaat atcttcattc cttcgataat
      721 acgttcgtat gcgtgccata ttctgggatt gccaagacca agtaagctca attcattatt
      781 gaaatccgga aggatctgcg ctgcttttat tttggacact tctacaccat tgtcggttcg
      841 tatggttttt attccatata ttttatcatc gaatgaaaaa gtaactatcc gggatataaa
      901 aaacctgaaa aacatactac aacacctgtc gatcaggtac aacagccggt ttttgttcca
      961 ttctttgtac tcgaatgcat aaggaaatgt tggtagttct agtacaatgc ttgtcttttt
     1021 aagtttgtaa ataagaagca taaccaaaaa caacggattc gtaagaaaat ggcggatata
     1081 caagccttgg taaccatttg caccggatgt tataggatat gttataaaca taaaccataa
     1141 taggctcaga cgatttgaaa atggatattt cttctccgag ttttcacttt ctatgacgca
     1201 ttcataattg cggaagtaaa gcaaatctac atccagccct gttttgcgca gtgcgcctgc
     1261 ctgtgcccgc acctttgagt gataacctgc attttgctga ttgccaaaat t
//
LOCUS       DAOQQY010000435         1246 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_80889_length_1246_cov_4.447523, whole genome shotgun sequence.
ACCESSION   DAOQQY010000435 DAOQQY010000000
VERSION     DAOQQY010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1246)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80889_length_1246_cov_4.447523"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="PLE23_13230"
     CDS             <1..971
                     /locus_tag="PLE23_13230"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HMX84277.1"
                     /translation="IDADTERGICRFRNTDGTISEVKADLFVGADGAGSALRNAFYKH
                     SASLRFSFSQQFLTAGYKELHIPPGNEGGFLLEKNALHIWPRGRFMMIGLPNLDGSFT
                     MTLFTDFDGENGFESLQAEDDVEKYFNKYFPDASALMPDLKKDFFMNPTGSLGTVKCW
                     PWQYGGKFALLGDAAHAVVPFYGQGMNCSFEDCVVLDQLIDQYGDDWPSILPKYQDAR
                     KQNTDAIADLAVENFVEMRDLVADPVYIKKRQLETRLEFNYPDYFSKYSMVTFRADLP
                     YSFAKEMGNKQNQLLMDICSTTSDISTLDTDAVMDQIRKLRTDRQE"
     gene            complement(972..>1246)
                     /locus_tag="PLE23_13235"
     CDS             complement(972..>1246)
                     /locus_tag="PLE23_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMX84278.1"
                     /translation="KVYLPKAFTPNGDGLNETFGISNPYVVNTIKTFEIYDSWGGKIF
                     ATTDVFAKWDGTFSGTPVSPGVYLYKVIYLCNNEERVQQGSVTILR"
BASE COUNT          357 a          261 c          273 g          355 t
ORIGIN      
        1 gtattgatgc cgataccgaa cgtggtattt gcagatttcg caataccgat ggtactatca
       61 gcgaggttaa agctgatctg tttgtgggtg ctgatggtgc aggaagtgca ctccgcaatg
      121 cattctacaa acattcggcc agcctgcgct ttagtttctc acaacaattc ctcacagccg
      181 gatacaaaga gttgcacatc ccaccgggta atgaaggagg atttttgctt gaaaaaaatg
      241 ctctccacat ctggccacgc ggacgcttca tgatgatagg tctgcccaat ctggacggta
      301 gctttaccat gacattgttt acagacttcg atggagagaa tggttttgaa tcattacaag
      361 cagaagacga tgttgaaaaa tattttaaca aatatttccc tgatgccagt gctctgatgc
      421 cggatttaaa aaaagatttc tttatgaacc ctaccggtag tctgggtact gtaaaatgct
      481 ggccttggca atatggagga aaatttgcat tacttggcga tgctgcacac gctgtagtac
      541 cattctacgg acagggaatg aactgttcgt ttgaagattg tgtagtgctt gaccaactca
      601 tcgatcaata tggtgatgac tggccatcta ttttgccaaa ataccaggat gcacgtaagc
      661 agaatacgga tgctattgca gacctggcag tagagaattt tgttgaaatg cgcgacctgg
      721 tagcagatcc ggtttatatt aaaaaacgcc agcttgagac acggctcgaa ttcaattatc
      781 ctgattattt ttcaaaatac agtatggtaa catttcgtgc tgacctgccc tatagttttg
      841 ctaaagaaat gggcaacaaa caaaatcaac tgcttatgga tatctgttcg actacttcgg
      901 atataagcac tctcgataca gacgctgtaa tggatcaaat aagaaaatta cgtacagacc
      961 gacaagaata actacctcag gatggttaca ctgccttgtt gtacacgctc ttcattattg
     1021 cacaagtata ttaccttata aagatagacg cccggtgaca caggtgtgcc gctgaatgtg
     1081 ccgtcccatt tggcaaacac atctgtagtg gcaaatattt tgcctcccca actgtcatat
     1141 atttcaaagg tcttgattgt atttactaca taaggattgc ttataccgaa ggtttcgttt
     1201 agcccgtctc cgttaggcgt aaaagccttg ggcagataca ccttgt
//
LOCUS       DAOQQY010000436         1240 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_81706_length_1240_cov_3.987342, whole genome shotgun sequence.
ACCESSION   DAOQQY010000436 DAOQQY010000000
VERSION     DAOQQY010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_81706_length_1240_cov_3.987342"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="PLE23_13240"
     CDS             complement(<1..1221)
                     /locus_tag="PLE23_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84279.1"
                     /translation="MCETAIANKGKTPVHEVKNEYINSPSKDFSGAFYNNMFVYGSGR
                     TDLVLPGKKLKLPGEGKAFNRLVTSTPDQNGYLKPPALFHSELQDQNNDMPVSFSTDN
                     KWVVFTKGSYYEHERPFSGTGKEHNLYIARVNDRGVWMDVKAMPFNVNGYSTTWPFLA
                     ENGQKLYFASNRPGGYGGYDIYVSQRFGDKWSEPANIGDKVNTAGEEISPFIMGDKMY
                     FSSDWYSVFGGFDICIAQKDDQKWQNPQVLLTGINSSYDDYAFVWNTDKNTGYFTSNR
                     EGGKGGDDIYRVRNIGAPYTLIVETPTGETIDLAVVDANDCGLSVMSTNTEGKVFFKT
                     LPKEKCSCTISLDGYETQKLEVTRDEVFGSQTRKVVLQPRIIEYTFQLIMKSNGEPVP
                     SIPVEISTQAGESVQ"
BASE COUNT          318 a          285 c          251 g          386 t
ORIGIN      
        1 ttggacagac tcgccagcct gagtcgatat ctcgactgga atggaaggta ccggttcgcc
       61 atttgatttc atgatgagtt gaaaggtata ttcgatgatt cttggttgga gtactacttt
      121 acgggtttgt gagccaaaaa cctcatctct ggtcacctcc agtttttgag tctcatagcc
      181 atcaagcgaa atggtacagc tacatttttc tttaggcaat gtcttaaaga aaactttccc
      241 ttcagtattg gttgacatga cactcaagcc acagtcattg gcatccacta cagccaggtc
      301 aatggtttca cctgtaggtg tttctactat cagtgtgtaa ggcgcaccaa tgtttctgac
      361 ccggtagatg tcgtcaccac ccttgccacc ttcacggttt gatgtaaaat aacctgtatt
      421 cttatcggta ttccagacaa aagcataatc atcgtagctg gagttaatgc ccgtaagcaa
      481 aacctgtgga ttttgccatt tttggtcatc tttctgtgca atgcaaatat caaatccgcc
      541 gaaaactgaa taccagtcgg atgaaaaata cattttgtct cccataataa aaggtgaaat
      601 ctcttcacct gccgtattga ctttatcacc aatattagcc ggctccgacc atttatcgcc
      661 gaagcgctgc gacacatata tgtcataacc tccgtaaccg cccggcctgt tacttgcaaa
      721 gtatagtttt tgtccgtttt ccgccaaaaa tggccatgta gtagaataac cgttcacgtt
      781 aaaaggcatt gctttgacat ccatccacac gcctctgtca ttgacgcgtg ctatgtaaag
      841 gttgtgctcc ttgcctgtac ctgagaatgg tcgttcatgt tcataataac taccctttgt
      901 aaagaccacc catttattgt ctgtgctaaa agataccggc atgtcgttat tctgatcctg
      961 caattcggaa tgaaacaaag caggtggttt tagatagccg ttttggtcag gtgtacttgt
     1021 taccaaccta ttgaatgcct ttccttcacc cggtagcttg agttttttac caggcaacac
     1081 gagatcggta cgacctgaac catacacaaa catattgttg taaaatgcac cactgaaatc
     1141 ctttgatggt gaattaatgt attcgttttt gacttcgtgc acaggtgtct ttcctttgtt
     1201 ggcgattgct gtttcgcaca ttttcaggta gtgcggagct
//
LOCUS       DAOQQY010000437         1213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_85246_length_1213_cov_7.102763, whole genome shotgun sequence.
ACCESSION   DAOQQY010000437 DAOQQY010000000
VERSION     DAOQQY010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85246_length_1213_cov_7.102763"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="PLE23_13245"
     CDS             complement(<1..784)
                     /locus_tag="PLE23_13245"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMX84280.1"
                     /translation="MKPKHILITGASGTVGREIVTQLLQNKNARLYVFDILNKKTKRF
                     YEEFKNHIHFIAGDISNETDIAKIPAGMDAAFHLAAIIPPAADDFPELAHTINTEGTR
                     LLVRHLEDTSPDCFFMYSSSVSVYGDRLDNPDIWVGDALVPSEGDLYAVTKIEAEKII
                     RASRLRWTIFRLTGIMKNHKISRLMFHMPLDTPFEISTPEDTARAFINGLEKQDELSF
                     RIFNLGGGAQSTISYREFLQRSFDIYGLGKLDFPEKAFAEKNF"
     gene            complement(895..>1213)
                     /locus_tag="PLE23_13250"
     CDS             complement(895..>1213)
                     /locus_tag="PLE23_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMX84281.1"
                     /translation="KIEEYVVGQKAYLDPNLSMEVISKKLKVPKYQLTEVLNTVIGKN
                     FFQYVNHFRVEEVKKMLSDKDNTYSIEAIGFDCGFASKSSFYTLFKNATGMTPTAYRD
                     SLA"
BASE COUNT          335 a          251 c          239 g          388 t
ORIGIN      
        1 gaaaattctt ttcggcgaag gctttttcag ggaaatcaag ttttccaagt ccgtagatat
       61 cgaacgaacg ttgtaaaaat tcgcgataac taatggtgga ttgtgctcca ccaccgaggt
      121 tgaatatcct gaagctgagc tcatcctgtt tttcaagacc attgatgaat gcccgggcag
      181 tgtcttcggg tgtagatatc tcgaatggtg tatcaagtgg catgtgaaac atcagcctgg
      241 agattttatg atttttcatg atgcctgtaa gtcggaatat tgtccaacga agcctgcttg
      301 ctcgtatgat tttttctgcc tcaattttgg tgactgcata caggtctcct tcacttggta
      361 caagcgcatc acccacccat atgtcaggat tgtccaacct gtcaccataa accgacaccg
      421 atgagctgta catgaagaaa caatcaggtg atgtatcttc aagatgcctg accaacaacc
      481 gcgtaccttc ggtattgatg gtatgtgcta gctcaggaaa atcatctgct gcgggtggaa
      541 ttattgcagc caggtggaat gccgcatcca ttccggctgg aatttttgca atatctgtct
      601 cattgctgat gtcaccagca atgaaatgaa tatgattttt aaattcttcg taaaatcttt
      661 ttgttttctt gtttagaata tcaaaaacat atagccttgc attcttattc tgcaacaact
      721 gcgtgactat ttcgcgacct actgtacctg aagcgcctgt aattaaaatg tgtttgggtt
      781 tcatttgcaa aaatttattt cttgcaaatt cgacactatt ctgagacaaa accgtccgtt
      841 tttcaatatc cggacaacca gtagccgtac gaactttaaa atccggattg tatgtcaagc
      901 aagagaatcg cggtaagccg ttggtgtcat cccggtcgca tttttaaata acgtataaaa
      961 ggaggattta ctcgcaaagc cacagtcgaa tccgattgct tcaatcgaat acgtattatc
     1021 cttgtcagac aacattttct tgacttcttc cactcgaaaa tggtttacat actgaaaaaa
     1081 attcttaccg ataacagtat tcagtacttc tgtcaattga tatttaggta cttttagttt
     1141 tttggagata acttccatac ttaagttagg gtcgagatag gctttttgac caacgacata
     1201 ttcttctatc ttt
//
LOCUS       DAOQQY010000438         1186 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_89194_length_1186_cov_3.748895, whole genome shotgun sequence.
ACCESSION   DAOQQY010000438 DAOQQY010000000
VERSION     DAOQQY010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89194_length_1186_cov_3.748895"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1186)
                     /locus_tag="PLE23_13255"
     CDS             complement(<1..>1186)
                     /locus_tag="PLE23_13255"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMX84282.1"
                     /translation="ADEARYVADLIFEQKMRHKIMHNEFAILYRTNAQSRSFEEALRK
                     LNIPYKIYGGLSFYQRKEIKDFISYLRLVVNPNDEEALKRIINYPARGIGDTSINRLI
                     VAADENQLPLAHFVDHPEIVSGLQGRAVTAIKNFSILIKSFRAMLNSKDAYEIAEYIG
                     KQTGIIEDLYKDKTVEGISRYENIQELLNGIKEYTVTDKQDISEDEVRPENDLAAYLQ
                     NISLLTDLDDEKDENKDKVKLMTIHSAKGLEFTSVFVVGLEENLFPSILSLNSREDLE
                     EERRLFYVAITRAKKFLTLTYALTRFKFGQLNYNDESRFIEEIDEKNKQVFGQKKPDT
                     AMKEKTFFDDDFQSKWYMSKQYQKKAEPVKTQAPAHLTKITKAARTQATLSSDLKNLQ
                     AG"
BASE COUNT          320 a          250 c          201 g          415 t
ORIGIN      
        1 atacccgcct gaaggttctt taaatcactg ctcagagtag cctgtgttct ggctgctttt
       61 gtaatcttag tcaggtgagc aggtgcttgc gttttcaccg gctcagcctt tttttgatat
      121 tgtttactca tgtaccactt cgattgaaaa tcatcatcga aaaatgtttt ttctttcata
      181 gcagtatcag gtttcttttg cccgaaaact tgtttgtttt tctcatcgat ctcttctata
      241 aagcgactct catcattata gttcagctgt ccgaatttaa atcgtgtgag tgcataggtc
      301 aaagtaagaa atttcttagc tctggtaatg gccacgtaaa ataggcgtcg ctcttcttcc
      361 aaatcttccc tgctatttaa tgataagata gaaggaaata aattttcttc caaccctacc
      421 acaaatacac tcgtaaattc aagacctttt gccgagtgta ttgtcatcag tttcactttg
      481 tctttgtttt catctttctc gtcatccaaa tctgtcaaca gtgaaatatt ttgtaagtac
      541 gccgccaaat cattttcagg ccttacctca tcttcactta tatcttgttt gtcggtcacg
      601 gtatattctt taataccgtt tagcaactct tgaatgtttt cataacgaga aataccttct
      661 acggttttgt ctttatataa gtcttcaata atgccggttt gtttgccgat atattctgct
      721 atttcataag catctttgct attaagcata gcccgaaaac tttttatcaa aattgaaaag
      781 tttttaattg cagtaacagc cctgccctgt aatccgctta ctatctcagg atggtcgaca
      841 aaatgggcta atggcaactg gttttcatct gccgcaacaa tcagcctgtt gatggaagta
      901 tccccaatac cgcgggccgg ataattgata atgcgtttca acgcttcttc atcattcgga
      961 ttaaccacta accgcaaata cgagataaaa tctttgatct cttttcgctg gtaaaaggaa
     1021 agaccaccgt atattttata tggaatattg agtttgcgca aggcttcttc aaaagaacga
     1081 gattgtgcat tggtacgata taaaatagca aactcattat gcatgatttt atgccgcatc
     1141 ttttgttcaa aaatcaaatc ggctacatag cgtgcttcat cagctt
//
LOCUS       DAOQQY010000439         1179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_90261_length_1179_cov_3.368327, whole genome shotgun sequence.
ACCESSION   DAOQQY010000439 DAOQQY010000000
VERSION     DAOQQY010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90261_length_1179_cov_3.368327"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PLE23_13260"
     CDS             complement(<1..269)
                     /locus_tag="PLE23_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84283.1"
                     /translation="MQAFFKWSILLFTTIWNVNFSQAQMPLKPSSGDIYNKIKSLQKV
                     GTVMYLAAHPDDENTRLITWLSNKQHLNTVYLSLTRGDGGQNLIGT"
     gene            complement(496..>1179)
                     /locus_tag="PLE23_13265"
     CDS             complement(496..>1179)
                     /locus_tag="PLE23_13265"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMX84284.1"
                     /translation="KINRGIRKQDNIVVGINQIATIVKVFGLGFWMMMLLDINIKELF
                     TSLSIIAAAIAIISKDYVANAISGMVLTWSDQLSINDYIKIEDQKGKIIDITLINLHL
                     LSDDDDLIYIPNNLAFSAKIINYTKIAVKKTSIEFELNTRNLTSIEELESKLINSLTD
                     YNKDIKPDSYNLKVYELKKNSISFKFQYILSHPDRELEKEIKKKTIRRILTILNDEKI
                     KATVNKTTV"
BASE COUNT          333 a          191 c          243 g          412 t
ORIGIN      
        1 gtacctatca ggttttgtcc gccatcgcct cgggttagcg aaagatagac cgtattgagg
       61 tgttgtttat ttgaaagcca cgtgatgagt cttgtatttt catcatcagg gtgggctgcg
      121 agatacatta ctgtgcctac tttctgcaga ctttttattt tattgtaaat atcacctgaa
      181 gatggtttta atggcatttg ggcttgagag aaattaacat tccagatagt agtgaaaagc
      241 aaaattgacc acttgaaaaa tgcttgcata tcagagaagt gtttgtgcgt ttttgttgat
      301 gaaatgtttg actacaaaca aatataaata cttgagctca taaattgttt atgtatgatg
      361 aaataaatac actacaatca tgcagtaaag gttgaataca actttgcctt gctttgtaga
      421 tatcgtaaac agttgaggtc tgaaatgaag cgatttgcaa taaacagtgg ccgtcataaa
      481 caggcaagca gtagcctaca ctgttgtttt attaacggtt gccttaattt tttcatcatt
      541 aagtattgtc agaatacgcc ggatggtttt ctttttaatc tctttttcga gctcacggtc
      601 cgggtgcgaa agaatatatt gaaatttgaa cgaaatagaa tttttcttca gttcatagac
      661 cttcaggtta tagctgtccg gctttatatc cttgttgtaa tctgtcaggc tgttgatgag
      721 tttcgattcg agttcttcaa tggaagtgag atttcgtgta ttcaattcga actctatact
      781 tgtttttttg acagcgattt ttgtataatt gatgattttt gctgaaaatg ccagattgtt
      841 tggaatataa atgaggtcgt catcatcact aagcaagtgt aggtttatca aagttatgtc
      901 aatgattttt cctttctgat cttcgatttt tatataatca ttgatgctga gctggtcaga
      961 ccaagttagt accattccgc tgatggcatt ggctacataa tcttttgata taatggcaat
     1021 ggctgccgca atgatggaaa gcgaagtgaa aagttcttta atgttaatgt caagaagcat
     1081 catcatccag aaacccagac caaagacctt gacaatggta gctatctggt tgataccgac
     1141 tacgatgtta tcctgcttgc gtatgccacg gtttatttt
//
LOCUS       DAOQQY010000440         1130 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_98159_length_1130_cov_6.205581, whole genome shotgun sequence.
ACCESSION   DAOQQY010000440 DAOQQY010000000
VERSION     DAOQQY010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98159_length_1130_cov_6.205581"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLE23_13270"
     CDS             <1..236
                     /locus_tag="PLE23_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMX84285.1"
                     /translation="IVEIGGHTNGIPDDEFCDRLSTARAKSIYDFLINVGVQPARLRY
                     KGYGKRLPLATNKTVDGRKKNQRVEIKILSLNG"
     gene            280..>1130
                     /locus_tag="PLE23_13275"
     CDS             280..>1130
                     /locus_tag="PLE23_13275"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002656150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HMX84286.1"
                     /translation="MKQAFIIDGVRTAVGSFGGSLAHTRADDMAAHVIRELVKKLPGI
                     DPGEISDVIMGCANQAGEDNRNVARMALLLAGIPYTVPGETVNRLCASGMSAVIHACR
                     AIRSDEGEAFIAGGVENMTRGPWVISKASTPFGRDAEMHDTSFGWRFINKEMQRMYGT
                     DSMGNTAENLAQKFGISRDDQDAFALWSQEKAAAARHSGRLGIEIVPIEVKKAKETIL
                     FDADEFIKPSTTAEILSRLNPAFRKAEAGGSVTAGNSSGLNDGAAALLTASEDFIKKN
                     NLKPLSR"
BASE COUNT          351 a          219 c          271 g          289 t
ORIGIN      
        1 tgatagttga aatcggaggt catacaaatg gtattcctga tgacgaattc tgtgacagac
       61 tatccactgc tcgcgctaag tctatttatg attttctgat aaatgttgga gtacaacctg
      121 cccggttgag gtataaaggc tatggaaaac gattgccatt ggcaactaac aagaccgttg
      181 atggcagaaa gaaaaaccaa cgggtcgaaa tcaaaatact tagcctgaat ggataatttt
      241 cgaaaaattc gtaccttccg ttcttgaatt attaaattta tgaaacaagc gttcattatt
      301 gatggcgtac gtactgcagt tggctctttt ggaggaagcc tggcacatac ccgtgcagac
      361 gatatggcag cacatgtaat aagagaacta gtaaagaaac ttccgggtat cgatccggga
      421 gagatttctg atgtgataat gggttgtgcc aatcaggctg gtgaagacaa tagaaatgta
      481 gcacgaatgg cactcctgct cgcaggtatt ccatatacag tacctggcga aacggtcaat
      541 cgactttgtg cttccggtat gtcggcagtt attcatgcat gtcgtgccat caggtctgat
      601 gaaggagagg cttttatagc aggtggagta gagaatatga ctcgcggccc gtgggttata
      661 tccaaagcat ctacaccatt cggcagggat gcagaaatgc acgacactag cttcggctgg
      721 aggtttatta ataaagaaat gcaacgtatg tatggcaccg acagcatggg caatactgcg
      781 gaaaatctgg cacaaaaatt cggaatctca agggatgatc aggatgcttt tgcactctgg
      841 tcacaagaaa aggcagctgc agcacgacac tccggcaggt tgggcattga aattgtaccc
      901 atagaagtta agaaagctaa agaaaccatt ctctttgatg ccgatgagtt cattaaaccg
      961 tcaactacag cagaaatact ctccagacta aacccagcat ttagaaaagc agaagcaggt
     1021 ggcagtgtca cagccggtaa ttcttcagga ttaaatgatg gagctgctgc attacttaca
     1081 gcatctgaag attttatcaa aaagaataat ttgaaacctt tgtctaggat
//
LOCUS       DAOQQY010000441         1037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_116614_length_1037_cov_5.816701, whole genome shotgun
            sequence.
ACCESSION   DAOQQY010000441 DAOQQY010000000
VERSION     DAOQQY010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116614_length_1037_cov_5.816701"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1037
                     /locus_tag="PLE23_13280"
     CDS             <1..>1037
                     /locus_tag="PLE23_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84287.1"
                     /translation="DARAFPDSYKKYGAWQPARIYFNTSYFFYKSQEEFDKADKSALA
                     NIEIGDYYPITGRSNNEIAAESRSMHKCQAFGTSGSRTEQKEYLEYLKGKKTGNKDDI
                     FEGLDLSWNRLKGGKPVNAMIENILKNYKFENPSLSIPDLVKLYQFINKNVENSRRKD
                     QKLEDLKEIIASAAGLFLEAAAVSAITTRSADVKIKTEITNRSPLQLRLNKISILPNV
                     WDTILLEKLLPPGGNMMLESNLITPSGINFTGPYWVADKPNNGLYVVNNPDLRGVPET
                     PRELKVVFSVNINGQDFEFGRNVIYKFTDPAKGEVYTPFDVMPDVYLNPVEDVVLFPD
                     NKAKNIQVKLT"
BASE COUNT          366 a          206 c          211 g          254 t
ORIGIN      
        1 cgatgcaagg gcttttccgg attcatataa aaaatatgga gcatggcagc ctgcccgaat
       61 ttattttaac acatcttatt tcttctacaa aagtcaggaa gagtttgaca aagccgacaa
      121 atctgcactt gccaatatcg agataggtga ctactaccct atcacaggca gatcaaacaa
      181 tgaaatagcc gccgaatcaa gaagcatgca caagtgtcag gctttcggca ccagtggtag
      241 ccgtaccgaa caaaaggaat acctggaata tctaaaaggt aaaaaaaccg gcaataaaga
      301 tgatattttt gaagggctcg atctgagctg gaatagacta aaaggcggca aaccggtaaa
      361 tgccatgatt gaaaacattt tgaagaatta taaatttgaa aacccatctt tatctatacc
      421 agatttggta aaattatacc aatttatcaa taagaatgta gagaattcac gaagaaaaga
      481 tcaaaaacta gaagatctca aggaaataat cgcttcagca gccggattat tccttgaggc
      541 agccgcagtg tcggccatca caaccaggtc agcagatgtg aagataaaaa cagaaattac
      601 aaacaggtct ccgcttcaac ttaggcttaa taagataagt attttgccaa atgtatggga
      661 caccatctta ttagagaagc ttttgccacc tggtggcaat atgatgcttg agtcgaactt
      721 aataacacca tccggcataa actttacagg accatattgg gtagccgaca aaccgaataa
      781 cgggctatac gtagtcaaca atccggatct gcgaggagta ccagaaacgc cgcgtgaact
      841 gaaggtcgtt tttagtgtaa atatcaatgg gcaagatttc gaattcggac gcaacgtcat
      901 ttacaaattt acagaccctg caaaaggtga agtctataca ccttttgacg taatgcctga
      961 tgtgtatctc aatccggtag aagatgttgt actttttcct gacaacaagg caaaaaacat
     1021 acaagtaaag cttactg
//
LOCUS       DAOQQY010000442         1019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Saprospiraceae bacterium isolate AMR_MDS_0406
            NODE_120871_length_1019_cov_4.544606, whole genome shotgun
            sequence.
ACCESSION   DAOQQY010000442 DAOQQY010000000
VERSION     DAOQQY010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810038
            Sequence Read Archive: SRR3501852
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Saprospiraceae bacterium (wastewater metagenome)
  ORGANISM  Saprospiraceae bacterium
            Bacteria; Bacteroidota; Saprospiria; Saprospirales; Saprospiraceae.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 08:25:44
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,658
            CDSs (total)                      :: 2,623
            Genes (coding)                    :: 2,616
            CDSs (with protein)               :: 2,616
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 2 of 7
            Pseudo Genes (multiple problems)  :: 2 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Saprospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120871_length_1019_cov_4.544606"
                     /isolate="AMR_MDS_0406"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2202734"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLE23_13285"
     CDS             complement(<1..367)
                     /locus_tag="PLE23_13285"
                     /inference="COORDINATES: protein motif:HMM:NF040248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMX84288.1"
                     /translation="MFSQLIYKLMYSIVVLLMVIITVSSIIYLAPVDPTRLSFGQMAD
                     AGTIEKKKERLGLDKPLPVQLMYYINDISPISIYRTSELSELPVTKSALSLSSTHSLV
                     LKMPYLRESFQSGRKVSELI"
     gene            complement(452..1019)
                     /gene="selD"
                     /locus_tag="PLE23_13290"
                     /pseudo
     CDS             complement(452..1019)
                     /gene="selD"
                     /locus_tag="PLE23_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
BASE COUNT          286 a          216 c          178 g          339 t
ORIGIN      
        1 ctatcagttc tgaaactttt ctgcctgatt ggaacgactc tcgaaggtaa ggcattttca
       61 aaaccaaaga atgtgtactt gacaaactaa gagccgattt tgtaaccggt aattctgaca
      121 gttctgaagt cctgtaaata gatatcggtg aaatgtcatt tatataatac attaattgta
      181 caggaagagg tttgtcgagg ccgagtcttt cttttttctt ctcaattgtt ccggcatcag
      241 ccatttgccc aaaactcaat cttgtaggat ctaccggtgc cagatatata atagatgata
      301 ccgtaatgat taccatcaac aatacaacta tactatacat caacttgtaa atcaactgac
      361 tgaacatgat atcttaaatg actgatataa gtgtttcttt tttctctgtt atataaccta
      421 ccggctttaa ctggatatca aaattctgta taaatgcctc gaattcattc tgcctttctt
      481 tttgaacagc aatcagtaag ccaccactcg tctgtgggtc acacaatatg gctttactta
      541 tgtctgtaag gccgtcaatt ttgtgaccgt atgaatcaaa atttctgttc gtgccacccg
      601 gaatacattt ttcactaaga taaaatgcga gtgcgtcctt gtcaagctga ggtacatctc
      661 gatatactac tgaagctgat acattgcttg ctacgcacat ttcactcagg tgacccagca
      721 aaccaaaccc ggttacgtct gtcattgctt ttacataatc aagttctgca agattctgcc
      781 caattttatt taattgaagc atagattctg gagcaagcct cctgtgttcc tgtttaattt
      841 ttcctttttt gactgctgtt gtaagaattc caacgcccaa tggtttggta agatatagga
      901 ggcaatcttt aatatcgcca ccatttttct tcattttttg aatgtttacc cggcctgtta
      961 cagcaagacc gaatattggt tccggactat cgatactatg cccgcctgcc aatggaatg
//
